LOCUS       DAOMFY010000001        53001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_173_length_53001_cov_12.702414, whole genome shotgun sequence.
ACCESSION   DAOMFY010000001 DAOMFY010000000
VERSION     DAOMFY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 53001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53001
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173_length_53001_cov_12.702414"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLB01_00005"
     CDS             <1..608
                     /locus_tag="PLB01_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HQQ75710.1"
                     /translation="AISGVASGGGGGGGTGGTGGVGTTAVGTGGNPVSAANTTVTGVC
                     GGQGSAGTVTVVTTHCAEWGGGGGGGSTATPTSSVGGSSLKGGGGGGTGGHHNATPAI
                     VAGAAGGASESYTGGGGGAVGTNGASPTAGSNGSGGDSTKGGSGGGGGGTTVTASTTA
                     GAGGNGGDNGGGGGGGGCGMNPGLGGAGGTGGRGALYVVTW"
     gene            761..2911
                     /locus_tag="PLB01_00010"
     CDS             761..2911
                     /locus_tag="PLB01_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75711.1"
                     /translation="MQSLGAFPQAATALIVALQLGRTVVPVQAVPYDPILTGKPREQI
                     AATAPYVRYHPRVETELARRQNLRPIVADAGGFLGGIEWGLAVLPRPQHPAEAQAVVQ
                     SMRGLPPPSTVAYDPLTSGKRQQGSPPSPPYPQVETAAAVAQAKRPIPFALAYDPLTL
                     GQQQDPAPAPLPFPQTETQYAAGAWRRLPVPQDAGQFLGGIEWGLMVLPGDRSVYGLP
                     VAQRTLPVPPAPVAAFDPTTLSRPAQAPPEAYYPRAEAALANARPSVSTDLDVLTAGR
                     RQQPPPPAAYYPQVETATVIARAALPVPFAPTYDLFASGERQESPPPPPYYPLVEVNG
                     LAVQLAKRPIPFALQYDPLTLGVQQDPEPPRLPFPQTETQYAASAWRRLPVPFAPQFD
                     PLTSGRRQTPPWLERYHPRVEAATALIPGTRTVIAPEFDPLTSGRRQGSPQPPPYYPD
                     IETRLVIRLNRQPIPFAPVYDPLTSGKRQAAPWIVPYYPAVELALAASEKAPVAPVYD
                     SLTSGKRQAPPPPPPYFPQVESQEANRRRVLPILETDILRSGRRISPAPLPPPYYPAT
                     ETALVVSLNARPIPPENFDVLTSGRRQDPPWLERYAARVETGLVVSLRLRAPVPEPPL
                     DTIISGGRQPNPQPPVYYPAIELAYANRRPLSALDVTVGVPGFIPPFGTAVDPDRGQA
                     DVLEGYGASVESSRRGITVYGGFGTSVESSRRKT"
     gene            2911..3246
                     /locus_tag="PLB01_00015"
     CDS             2911..3246
                     /locus_tag="PLB01_00015"
                     /inference="COORDINATES: protein motif:HMM:NF022117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HQQ75712.1"
                     /translation="MREHFMGQGDLLPEIYADLTDGNGNPLDLTTATAVLFYMDLETG
                     AHKIDGVPGAVVGDPTLGRVRYVWTGTDTDTSGVHRAQFRVTFPTALPASFPNDDYIY
                     VWITPKVGS"
     gene            3355..3678
                     /locus_tag="PLB01_00020"
     CDS             3355..3678
                     /locus_tag="PLB01_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75713.1"
                     /translation="MEAVEHVRDEVILRIDASDKITDQKIGRVADKVDAVEGKVTEAK
                     QIAATGIAETHIAIGNVKGQVEKVDAKVSTVQLTVAKMIGWAAGAGAVAGAVVGIVVK
                     LLSHS"
     gene            3675..4121
                     /locus_tag="PLB01_00025"
     CDS             3675..4121
                     /locus_tag="PLB01_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75714.1"
                     /translation="MRFASPRIAAEWQGADLKGVRVVPALIGIVEDAADFVRSLYAWE
                     LMLTCLLRTPEENDALYVGRGYHPGTHLDGVHVRGRGGDARIRDVDPEAVRGLHGYLN
                     ERYIYDPERPTLSVALLEDGITAGSGAHLHLQVHPRTTNRHFTQEP"
     gene            4277..5056
                     /locus_tag="PLB01_00030"
     CDS             4277..5056
                     /locus_tag="PLB01_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75715.1"
                     /translation="MKWIPGKRMQCPICLRDFTQTEPNQETHKACAGRVNRGIASNPK
                     ELSTPYRDAGYTPGKQIPKWKAEPTLKSPMRIAVLDLETFALDRGWGVLLVGVIVVFG
                     DGEPKEYRFRLDKFDTYKRDRSDDSELAASVLSVLEDCHIWYGHNSKWFDIPYLNSIA
                     LKYGMPPIERKLRDPVQLLRAKFRIGANSLEAATDFLGLEQSKMHVPAEVWRQAAFNG
                     SKAHFDILEERCVSDVHLLIELARRLEDWGGVVDYSGFYRR"
     gene            5053..5493
                     /locus_tag="PLB01_00035"
     CDS             5053..5493
                     /locus_tag="PLB01_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75716.1"
                     /translation="MNLKELAWAAGFYDGEGSTCGGVRPDRARGTRLMMSITQTDPRV
                     LKRFQAAVGVGRVNGPYDFKGNRHRQAPQWAWQCQNWRDVQVAVAKLWRFLGPVKREQ
                     IQRAVAASRIAARSEARLAVSLYAEYNRGWDECHGTVPPAEEKK"
     gene            5490..5735
                     /locus_tag="PLB01_00040"
     CDS             5490..5735
                     /locus_tag="PLB01_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75717.1"
                     /translation="MNMDKCSYPEGFEDFINARYAEPWFNEPHKFTHVRGIELARLAW
                     LAATDRERRRCVEVTWSVRCRGEHCDCYDAIRKEINQ"
     gene            6299..6613
                     /locus_tag="PLB01_00045"
     CDS             6299..6613
                     /locus_tag="PLB01_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75718.1"
                     /translation="MISQRRAEILATSFAIAGMGTPGRTSAGGVSMSGRILTREEYAD
                     AIDMVHATEKRELLERFDVGAERYGILDLSLKDWEREEHEERLDKKAYKIFDWVRKAR
                     GL"
     gene            6613..6993
                     /locus_tag="PLB01_00050"
     CDS             6613..6993
                     /locus_tag="PLB01_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75719.1"
                     /translation="MGVIPYSFTLWGAEWRVVWGWTGPQHWRGFRWNPKRLAGLCVFE
                     PLKGNRIIILNPTLKSGSAIKRWEAFAHEVLHAITYEARFRRVSHWKGRDLSHSVIDR
                     LEFPLAVFLMENARFGGENERAGS"
     gene            complement(7040..7261)
                     /locus_tag="PLB01_00055"
     CDS             complement(7040..7261)
                     /locus_tag="PLB01_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75720.1"
                     /translation="MNDQDPDIPRSLGPRVRIVFHPEGRQEPICTRHKKDVTMICPAC
                     RGERGGKTPSAARLKALAKNRKKRWPKKG"
     gene            complement(7258..7464)
                     /locus_tag="PLB01_00060"
     CDS             complement(7258..7464)
                     /locus_tag="PLB01_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75721.1"
                     /translation="MTMNTHYGCDNCSDEIQVDDDTRETLTLLIPLEGTSSAEVYRFC
                     SWSCLAEEAEARLKTERTNNGGRG"
     gene            complement(7688..7882)
                     /locus_tag="PLB01_00065"
     CDS             complement(7688..7882)
                     /locus_tag="PLB01_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75722.1"
                     /translation="MDSLVARLATAPLRSMELRKDWKFSRACARNAAHWAIAVVYQRT
                     FGRIPKGTSTEAMAELMGAR"
     gene            complement(7998..8070)
                     /locus_tag="PLB01_00070"
     tRNA            complement(7998..8070)
                     /locus_tag="PLB01_00070"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8035..8037),aa:Ala,seq:cgc)
     gene            complement(8267..9433)
                     /locus_tag="PLB01_00075"
     CDS             complement(8267..9433)
                     /locus_tag="PLB01_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQQ75723.1"
                     /translation="MAWIRSRRRNGRTFYLVRDRQGRERSCGSGPKGAKLARRLVGQL
                     EKAEWEERVGLPVSARLAASWTLEALRDRDVAGLAQGHSRRRRWTVLLEGLGSATPLD
                     RITPDAIRAYADARLAKAKPQTVNNDLSVLCSALRFAAERSRESGFQADPCREVRRLR
                     PAPRRLALAQRPEAVAALIARAWAKAREAPAHLEDAWRDNAAMIELAYETFSRISQIH
                     GLERAWVGPEFLRFPPQKGGNARHFRIVGRLKELLELIPERGPYFFPGRGGEGHRDNF
                     RRFWSKIAPAGMTPHALRHSAVTNALYAGASIPEVAARCGHKTPQMVARTYAHIFPAA
                     LEPMSGPPGKPQRKRRASGHPGATHGRTGGHPGTGRKARKPSGIRISGDPERPN"
     gene            complement(9434..9757)
                     /locus_tag="PLB01_00080"
     CDS             complement(9434..9757)
                     /locus_tag="PLB01_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75724.1"
                     /translation="MKTILGILFAVATLGCASANQTEATYALRYRVNVTSNPSSVSSC
                     RRIEGIDINSAPCATMIVGGGQTPSPMDCARYWTVREGGDTLLVGSKTGDIYLCKAPI
                     QTAAK"
     gene            10250..10387
                     /locus_tag="PLB01_00085"
     CDS             10250..10387
                     /locus_tag="PLB01_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75725.1"
                     /translation="MKPIPAKAWASTITKAQRNARKRNSTLSALVRRFVEELAREDAA
                     A"
     gene            10384..10506
                     /locus_tag="PLB01_00090"
     CDS             10384..10506
                     /locus_tag="PLB01_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75726.1"
                     /translation="MIDEELDDPCDGGRCHREESGTWLPCRKHEQHDRDDEAES"
     gene            10563..10817
                     /locus_tag="PLB01_00095"
     CDS             10563..10817
                     /locus_tag="PLB01_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HQQ75727.1"
                     /translation="MTVPEAARQTGFSRWTILRAVRDGRIRAVQLGRAIRIAKPDLDA
                     AVREWERSAVAVSVHAPTYTDAVIDRVFNRSRAASRKGTR"
     gene            10999..11121
                     /locus_tag="PLB01_00100"
     CDS             10999..11121
                     /locus_tag="PLB01_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75728.1"
                     /translation="MKASTIETVAAIGVRVLVVIGVMVLIALATLAAVKMGGFR"
     gene            11204..11413
                     /locus_tag="PLB01_00105"
     CDS             11204..11413
                     /locus_tag="PLB01_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75729.1"
                     /translation="MTIICASCKKPCEGHKHTNAGWLCRSCVLSASTVLALYIIDGVT
                     YVEKLKTPAVLAAVPVERERQAVAR"
     gene            11410..11571
                     /locus_tag="PLB01_00110"
     CDS             11410..11571
                     /locus_tag="PLB01_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75730.1"
                     /translation="MNRDEQLDAIRAWFCARQLHHVTNATITRRVTVVNTICSAISAY
                     LGAHRGGAR"
     gene            11568..12548
                     /locus_tag="PLB01_00115"
     CDS             11568..12548
                     /locus_tag="PLB01_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75731.1"
                     /translation="MSDTALVTQSDLHADALARINETVGSLRALRTFIKAEMVKGVDY
                     GEIPGTKKKVKTEDGKTVEVPAQALLLAGAQKTTMYFNARPRLDVRGTELAGGHVEYL
                     VVCDLIHRGTGNVIAQGIGSCSTMESKYRFRNASRVCPGCGQATIFKSKEPKPGKGWF
                     CWRNRGGCGLEFDGAAPEIVKQDNGKVENENIHDQRNTVLKMAKKRAQVDAAMGLGCM
                     SELFTQDVEDFDTYEIPDEGFAAGEIPLDAPPPAANEVPKAAPPAGAKRPWLEVMAEQ
                     KGRVGAEAYLKVLNDSEFDRAEQITEVADRARIYKELLAIPGVQQPERAK"
     gene            12549..13124
                     /locus_tag="PLB01_00120"
     CDS             12549..13124
                     /locus_tag="PLB01_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75732.1"
                     /translation="MELVLTPDHRYILNGRHIPGTTEPLVGLGLLDTRYFTEFARERG
                     RAIHAAIHYHLTGGVDWATVDPRFVKYVEAALRFIDDANFTPHGVEVVKASEHYGFGG
                     TLDTYGIGFGELILPDWKSGFLGEITGLQLAAYELLLGVEARRMAVQLRPDGTYKKTD
                     FTKRQDRPRFLGALDLYRTFIFKEGEERVAA"
     gene            13111..13782
                     /locus_tag="PLB01_00125"
     CDS             13111..13782
                     /locus_tag="PLB01_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75733.1"
                     /translation="MLQHEPIEVPDAGPMKQEGLAIVEQALAVHITDPESHKAAAEQL
                     VHIAGMVKRVKAAFAEPKRLAHAAHKSITALEGEALEHLEKADRLYRSAISRWLVEED
                     RRRAAEQARIEAEERAKAQDAAIAEAVALEAAGDIKGAERVLAEPVLAPVVEIPKTVT
                     PGVSQGRKSYDFRITDPAAIKRQYLIPDEVAIRKIVKAMGPDAATVIGGIEVLEERIV
                     SVRGN"
     gene            14039..14311
                     /locus_tag="PLB01_00130"
     CDS             14039..14311
                     /locus_tag="PLB01_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75734.1"
                     /translation="MRMFRFSQWRPYGHDWKSWMSWFRRSSGSYAEVRRCGSFTVPPS
                     ERHRHAPREEGHTAIYVLGEDAFDLNPVEGLEVEDVDGSTVPLGNG"
     gene            14319..14555
                     /locus_tag="PLB01_00135"
     CDS             14319..14555
                     /locus_tag="PLB01_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75735.1"
                     /translation="MVAVLLAVWFGWLAPSRRNAAVAPAPRVEATYENTCKIEKFDDE
                     AGPKWKCYQDGVKVPCPRALPKLRNCNATEDVIR"
     gene            14552..15541
                     /locus_tag="PLB01_00140"
     CDS             14552..15541
                     /locus_tag="PLB01_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQQ75736.1"
                     /translation="MSGRELFVKIVSLCSVAILFLLLFFAVAVVLIFGVGSCVGQPLP
                     EIKFTEAPYSHTGFATSGCVYSGQTCVHVQQWGAYALNVVTGGFKMVSPIDKGGQCVQ
                     APKFIVAGDCHQSGVTVAASPGAEKWVVVTGAPNDQCNPDHGQFQILRLDGASGLRWV
                     GEAFAPAVGRVNTAPSIDGDFAYAVEGNGQTFTRVDLNTLAVTSGLGSRPPWVPQPNV
                     GGYTYAITVDPGFPQVCGAPQHFIATRSGSTPPPTPPPTPTPGGNPFATYIEALARAG
                     VTAGCGGGNFCPERTMTRQEMATWLVLAINHGGPMPLLACQGTFNDVPCGSAP"
     gene            15538..15771
                     /locus_tag="PLB01_00145"
     CDS             15538..15771
                     /locus_tag="PLB01_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75737.1"
                     /translation="MKAMVSLGDNVIEFDTSEPPAEVSILGVSYRRVRAGEAERVVML
                     SEVRLLKMEKRINDLETRVRHCEEARGLVRGPR"
     gene            15768..16031
                     /locus_tag="PLB01_00150"
     CDS             15768..16031
                     /locus_tag="PLB01_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75738.1"
                     /translation="MMRICQGPRCGRQFPPNPGERGTKRRYCSRACYVRAKLWRQQGR
                     TWKVAAGDVTREILDSLGHDDREPGEGPDYTFPDFTTFDEAMA"
     gene            16028..16363
                     /locus_tag="PLB01_00155"
     CDS             16028..16363
                     /locus_tag="PLB01_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016046212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75739.1"
                     /translation="MSAAEPEQVGLFDGVEDTRLEREFRRFHFENPEVYRELVALARR
                     AKQHSRNRYGIGALFEVLRWNRMLSTTGDDFKLNNNHRAYYAREIMRREPDLSDFFET
                     RHVHGEEQA"
     gene            16474..16794
                     /locus_tag="PLB01_00160"
     CDS             16474..16794
                     /locus_tag="PLB01_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQQ75740.1"
                     /translation="MKGRTGAEHPAFKGNRPIADRNGYLRVYDPTHPWRRNKGIIQEH
                     VRIMELHIGRRIAPDEVVHHRDHNRQNNNLENLQLLKRGEHSSLHRREDSHTRKRDTN
                     GRFA"
     gene            16835..17485
                     /locus_tag="PLB01_00165"
                     /pseudo
     CDS             16835..17485
                     /locus_tag="PLB01_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            17641..18036
                     /locus_tag="PLB01_00170"
                     /pseudo
     CDS             17641..18036
                     /locus_tag="PLB01_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            18033..19205
                     /locus_tag="PLB01_00175"
     CDS             18033..19205
                     /locus_tag="PLB01_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75741.1"
                     /translation="MTGPEVARSLSSQRDGYNDGSDQTYIAAPLSHGSNPNSNAAGRR
                     REDDVNLAVVPALTSKMAKGTGGPAGDECQNLVWQGGSQQDQIIEEGGVVPTLAHSAN
                     THQGNHQPKVMNSHGVRRLTPTECERLQGLTDIENAAIIEVCLCAPRNADADAAAQCR
                     RSRTNASDADGVGSPLDASSVGLSSASCRRDPASVAPLRVRIVSGEREAEIASVSAPS
                     SGAKDAGENFSRRRVVRDDGFALSLARLTRNVALSIHPGEEASPRSGPCSTPPSNGAP
                     RVRLCGSEITRLAADADAASIIPSTPTTPTTSAPSRIEITESDLETSSLSALRAIAGF
                     IPAETWRESFSISIKSRVGWTFPGADSRRYAGLGDAVTANVAAWIGERLARLGGEA"
     gene            19202..19792
                     /locus_tag="PLB01_00180"
     CDS             19202..19792
                     /locus_tag="PLB01_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HQQ75742.1"
                     /translation="MIEPTQEGFWGHLEPPYRTIVADPPWRYASAATKADARKHYSTM
                     GLADICAMPVAALADESAHLWLWGTNALMYEAHLVCRAWGFRPLTVVTWCKAGPGVGH
                     YLRNNTEHVILASKGKPATPKEKPLSTWFQWEAGQHSAKPGAFFDLVERVSVGPFVEI
                     FARQPRLGWDHWGKGFESRIANVEPRLAEVAMEASS"
     gene            19789..20760
                     /locus_tag="PLB01_00185"
     CDS             19789..20760
                     /locus_tag="PLB01_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HQQ75743.1"
                     /translation="MSISKIEWTDRTWNPVRGCSRVSEGCRNCYAERFAARYSFDGQA
                     FHGFARFGASKREGGTITNRPEWTGRVELIPEKLSEPLRWKKPARVFVNSMSDLFHER
                     LSIDAVAAVFGIMACAPMHTFQVLTKRPARMRAFFDRIQSEPRFAMSVGLSVAPANYP
                     HALNFPAVWPLPNVWLGVSVEDQATADERIPLLLQTPAAVRFVSYEPALGPVDFDRLV
                     IPGGTMAPLYGKGLDWVIVGGESGPGARPFAIEWARSTVRACRAADVPVFVKQLGAEV
                     RIDGPSDHVHFAGAFDPEKQFFPRLRDRKGGDPAEWPSDLRVREFPR"
     gene            20757..21290
                     /locus_tag="PLB01_00190"
     CDS             20757..21290
                     /locus_tag="PLB01_00190"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HQQ75744.1"
                     /translation="MSPRSPELYPENAGATDGPSIMGRIDFVPPTTSHHAKKIVRFQV
                     GGRQITKLGDTDELKAARAIWWLVLLPFKPTEPLEGPLAVTLDLTWPWRKSDSNRTQL
                     LGRIPSEVKPDADNAAKGILDTMARLLFFKGDQQVADLHIRKWIGMRPGLEFSIRKIQ
                     FGGERGAVPASGQEVRP"
     gene            21250..21978
                     /locus_tag="PLB01_00195"
     CDS             21250..21978
                     /locus_tag="PLB01_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75745.1"
                     /translation="MERFLRPGKKYALEIVDFNRVIVRSQALREAKARGRLPFVTIPT
                     KNGAGLMEVLSHDHGLALYGAFVLLARAAADMEVRGLFARHGRPLTTPEIARLAMIPT
                     EEADAHVARLIKVGWLRFSELNSEQNSEVASEVNSEVSSDGREGKRSDNEGRESTRAE
                     RRPRDWQEVTEFWTANGFLGDPVAFWEWNDDRGWKWKSWKRAAAAWSRREGRRATPRS
                     SGPKDYEAGAAESNAEVAARGVIR"
     gene            21975..22868
                     /locus_tag="PLB01_00200"
     CDS             21975..22868
                     /locus_tag="PLB01_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ75746.1"
                     /translation="MSAAEFDPLFEIPEADPVTLHEPPTQVTPPHGRFFADLAIPPRI
                     REFLTPKPWEAGILMGPAGTGKTAEACARLVGASVAADRAVGLFVSIPELLHRLRAAV
                     GDPAAQDYELDLVLAAKHLVLDDLGAEKVTDWTRDRIYLIVNYRESRRLPTIVTTNLN
                     VAGIRAFDERIASRLLALAPVQTLTGSDRRTWRSEPLEQNAPFLTDKPAPPKPAEPYV
                     APPTPEEIKRVEKAFGRIYANMGLKGGAAEFAALVQKAEPEQRLMPRGDFNGRVRELV
                     RRATAGEKFDIRAELEATDAA"
     gene            23026..23229
                     /locus_tag="PLB01_00205"
     CDS             23026..23229
                     /locus_tag="PLB01_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75747.1"
                     /translation="MRAHRVTTDFDGESFVIRRRLTCRNCGARAMSREKITLFVISEN
                     ERAFYAGKDPESFRPTDFDSPEK"
     gene            23296..23628
                     /locus_tag="PLB01_00210"
     CDS             23296..23628
                     /locus_tag="PLB01_00210"
                     /inference="COORDINATES: protein motif:HMM:NF039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HQQ75748.1"
                     /translation="MTVRGRPFPKGASPNPGGKPKQKPITEAYRRLVESSAEALEKLD
                     KKNLTGADVVALAIFRQAAKGNALAAREMTDRLEGKVAQPVDANVNLGEAFAGIVQAL
                     AAKKGRAS"
     gene            23625..24101
                     /locus_tag="PLB01_00215"
     CDS             23625..24101
                     /locus_tag="PLB01_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75749.1"
                     /translation="MSSNAPTPPPPPVLPDDGVALAPHLVQRISFLSNRMRKDPQEIV
                     NAVLSIGFHAIDRRTMDDVVAEFSAEFTAAKSAKMPDPEKKAPDPDDQVEETAEQRFE
                     RMRRNRALARGEDPDAPPACNLGIQGCVTVHGDQAEKEPGLDERHLDLLAQKAAGK"
     gene            24098..24574
                     /locus_tag="PLB01_00220"
     CDS             24098..24574
                     /locus_tag="PLB01_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75750.1"
                     /translation="MTVLALLAAPTLAAWIFVGLLVLAGLAIWLVCRGASNPAPVEVK
                     ACRDLHCVCQVLKALARMEKRLEKVEEQLMGVNANIQELSDRIVELKAAQADSAQKVT
                     DAFARLEAAIAAGGQLSEEDKAVVAAAKDSVVAMTGAEQAQSAQADAEAPPAAPEG"
     gene            24670..24852
                     /locus_tag="PLB01_00225"
     CDS             24670..24852
                     /locus_tag="PLB01_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75751.1"
                     /translation="MKRKPKPTPEPAPPVASLAEELLAIRVRLHNLEDRVYYPGGCEL
                     HPNVPGARCEPTPEPD"
     gene            24852..25007
                     /locus_tag="PLB01_00230"
     CDS             24852..25007
                     /locus_tag="PLB01_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75752.1"
                     /translation="MNPVAWTIGLTYVPGCGGWGIRFVHDAPELPSEVVWGIRFGPFV
                     LLKTRPR"
     gene            25191..25934
                     /locus_tag="PLB01_00235"
                     /pseudo
     CDS             25191..25934
                     /locus_tag="PLB01_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            25918..27333
                     /locus_tag="PLB01_00240"
     CDS             25918..27333
                     /locus_tag="PLB01_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75753.1"
                     /translation="MSAQPDLRPLADFIEATYDDPALFITYGLKTHLRTWQAKVCEQY
                     RQQLVAGKTAIKGTLRTCHGSGKTFLVAGLLLHWMTTRYNSRGLTLAPVFSQIESALW
                     PEIEKLHHRSIMRSFGFGRMLNTRIDFGATWFAEGRSSDRPGHLEGQHSDFAAIRLID
                     EAKEVDPAVFDATDGLLNSPLSCDLWISTPSLESGKFYERDMGPDPDVIRAVITVDDL
                     INDPEMTEAERQGFARWKAERLEEWGPNSAEYQSRVMAQYAENAEGALFPSSWVERAV
                     KADWSVDERDEFGQPHPVMGGMDVAGSVDGDRTAVAIVRGPDAKRRHELLPSMKWWHE
                     QDTTKSRTRATEIAKVHGAFLRIDSIGLGKGVLDGARELGFPAQEYRASDKPMDQERF
                     TNRKAEDAWRVRTLLEQGLLKLPNIQELKADLRGMKYEIKPNGKIHVVDPPKSPDLAD
                     ALIIALGRSRVGITSASPEWL"
     gene            27662..27883
                     /locus_tag="PLB01_00245"
     CDS             27662..27883
                     /locus_tag="PLB01_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75754.1"
                     /translation="MRVVESTLRIRLELTPESPFFLEAESVIRARLLTAFNEGARVWA
                     LRTDDQLVNARPEYAPDRHLWPFQNERRA"
     gene            27880..>28130
                     /locus_tag="PLB01_00250"
     CDS             27880..>28130
                     /locus_tag="PLB01_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75755.1"
                     /translation="MSRRRTVAEPVLCACGCGEFVRRGRTYLVGHHDTPRARQLELGP
                     NSRRRDYTGEREVRMIDCFDSYAGEAMADGFALLGQDGD"
     assembly_gap    28131..28230
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            28295..28525
                     /locus_tag="PLB01_00255"
     CDS             28295..28525
                     /locus_tag="PLB01_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75756.1"
                     /translation="MNALAFYAGAMTILAVAGYLAWYGSTMQNKKLLAAILAVNADRE
                     RLAAELGATIAKHNAEKSAASRQVGEGDPAPN"
     gene            28525..30225
                     /locus_tag="PLB01_00260"
     CDS             28525..30225
                     /locus_tag="PLB01_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75757.1"
                     /translation="MAEPTPGSDKPASRVEIPAEAELNFELAARYAKKGPNPEYKAFE
                     ELVDIASFSSPWLSVQTDIWQFIEDAVEGKGGFVDGTYLIPFKREVKRRGGKIDDKFY
                     ERVAASDYDNFAKTICDGPWNYISQNRDAISRDTKDAELQAFWNDVDGNGTAMVDFLE
                     YPVRQASMYGTGWIFMDRPNMGPDATIADSSAPENRPYIYGVPTRNVVDWVFDENYEL
                     QAVAILEHDGDDDEAKDDPTKCNLRVWTKTSFILFVKGDSDHYSVDLKRSGPNTLGEI
                     PCVQLFNEYPGPGRGLGGTIMPAIARLARSHYNMRSEAREIQRKCAAFLAYPTKDLDA
                     GSKLEIGSGSALPFNADAGKPEWVTPDLASLEHYREDMKATKESAFSMSHMSAISGFM
                     ETDQTSSGFHQQAVFDDTNKQIGKRAGGVEAAEVKLARLFQRMRGIGGEQPTPADEKK
                     IKDSFSISYPREFGLRDMSAELNRLGKRLELEMGKADAEEAIFDFYQSAYPRADRKQL
                     RARAQQSAADYYDSLAQANAANVVPMTPRDRAKAIATKAVNGETFSSKPKARPDVPAA
                     "
     gene            30239..30973
                     /locus_tag="PLB01_00265"
     CDS             30239..30973
                     /locus_tag="PLB01_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75758.1"
                     /translation="MTENAAVAEPIESDATTDTPDDREGAPAKGLTEDQLKAKRYSAE
                     AASWRKKLRTAEARIKELEAGLGSGDDAGDDNGSPTGQKRSPSYIDSELASLRRQVKE
                     ANAKVEDISGKWTKAEEKARRVAHENLAGRIVAGLNSPNTAIELLLSKTKLSEDEKTI
                     VYTTKDKETGEMVEVEVTKESLKSSGLLDSIFFPAEGANGNGTGTTAPVTGRGHKLDL
                     SKIDDAAYFKEHREEILALRGKPKRA"
     gene            30989..32185
                     /locus_tag="PLB01_00270"
     CDS             30989..32185
                     /locus_tag="PLB01_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75759.1"
                     /translation="MSNALTSVTTDLSVFGNLVLEQLQPRLVMLRRITVGVPVPATYG
                     DEIQISDISVSGNAGTRAVGGAATASDLVALTRTITKTNIYKAVKLDNLEIFFSNIGL
                     MERAARQIARAVAREVDAVILRLVDTDVPYEVGQLDGSSCMDEAQKYLSLAYKQLMKN
                     GADVESLFCVLGDSEAHNFRQNSNLYKVNEAGSSDLLRRAILGSVLGFEIVESQQVVS
                     STLSTNAATTGTASVSAASLGASSISAASLGTGTIKKGTTFTISTVNNALGNSIGFVV
                     TADATITANAATLPIYPSLPVALAGTETLTFVDHSAAGSMNLAWDRDSILGYVAAPAP
                     FPEGTGVASTTVTDEQSGLSIRIAAKSNLLGSAGNAYNTELGADVMFGAKVIRPEGTV
                     KITGEV"
     gene            32190..32636
                     /locus_tag="PLB01_00275"
     CDS             32190..32636
                     /locus_tag="PLB01_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75760.1"
                     /translation="MRVYDITQKNVSLTAAVQTVIEFTAATNQTVVVLRAWISQSGST
                     TSAQQNVNLIRKTAAGTNATAPNANPVDASDTAFAGTVRGLCTTEGTLGVDLIADSFN
                     WVNGWIWLPVPEERIYVKGGAILGLRLPTAPPALTCNAGITVGELG"
     gene            32904..33239
                     /locus_tag="PLB01_00280"
     CDS             32904..33239
                     /locus_tag="PLB01_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75761.1"
                     /translation="MRGMRFTSKPRLAVEERGLRLDPNDFRSARERANLTIPQLAEKL
                     GLEEHVISGIEKGYRVLSPLQAKVLYLRIELLEQARLEAQAKAAATAPPAMRVKLPQM
                     TEREEVANG"
     gene            33232..33642
                     /locus_tag="PLB01_00285"
     CDS             33232..33642
                     /locus_tag="PLB01_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75762.1"
                     /translation="MADPLIWVRKPGQPPVTCYQSAIRFALSNGYEECSPPPAPQVVP
                     TAPTGAEGAAKKGEQLSPPVAAKPSAYARSRRPGRAKKFTEELRRLLKFYREHPGSTV
                     AQAVEALGTDPTWSELNTADLFANGYLELVEVAA"
     gene            33639..34109
                     /locus_tag="PLB01_00290"
     CDS             33639..34109
                     /locus_tag="PLB01_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75763.1"
                     /translation="MSDLTDEQILTIRESYLRDRYVRAPVTIAISNYNRSGREFFTSD
                     QIKAIDDEIAVTWREVLDDVNAEQVAVRINEGDSQDTADDSGDAMELIARYKLIEAAG
                     IEKMLEDPGFRASLIPGDDAKGAEAMKSWEARIFRNRQWVMGRAGRAAIEVERK"
     gene            34106..34363
                     /locus_tag="PLB01_00295"
     CDS             34106..34363
                     /locus_tag="PLB01_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75764.1"
                     /translation="MSNITPSRGLASHAETMVKTGRWKSDRQKAAEQRRHLGVPARCQ
                     DCRDGIHAHRGPMGCLEVVGSPDTYPPRDFVCQCMKQEEVA"
     gene            34363..34722
                     /locus_tag="PLB01_00300"
     CDS             34363..34722
                     /locus_tag="PLB01_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75765.1"
                     /translation="MGFDPNQPRDEKGEWTAGGGGSGKMETHGGGGHVHESKMKGDWK
                     KGNDSGEQTVLKSFSSRSKAMEVAKDSGLDPAGVRRGKFGFEHPGLRAPVASQAQVEH
                     MKQSNSRGLSSVAPFKK"
     gene            34723..35568
                     /locus_tag="PLB01_00305"
     CDS             34723..35568
                     /locus_tag="PLB01_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75766.1"
                     /translation="MADPKEALRYVESSEYRKEYARQLAKRLAADMDIRRVIDEGGDY
                     AFSLLDDLVGKIKESLLVDAVADARAHAKALGLEGLSDFEIRQVVAASVENVGTEAMH
                     GLEARLAELDRALTRMEDGGASLATIRASVTAEGAAKGLLSGLSSVLTSTAAGIVADI
                     ESNVLRSAAFGTDQKMREDDPDAEPPLWRWETREDDKVCEDLYENSCAPRHGDELTME
                     EWGAFGEPQSPNLICSIYAKGAFSNCRCVLEPANAAANPEPLNITEAAARGRERAQAA
                     FPEVA"
     gene            35568..36167
                     /locus_tag="PLB01_00310"
     CDS             35568..36167
                     /locus_tag="PLB01_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HQQ75767.1"
                     /translation="MADVLSQSPSGVPLRSPLQVTVEWSGEAGKALLSAARRLGRVEP
                     LLPVIGRNLVNSMRKNILENRTPEGKAWPVLKRDRGPGRNPDRRALFDSGKMYDAITY
                     RLLDAETVSAGIYDVPYAKFQNQGVQKSWTIRPRRFGSKLRFWGGDGWVYRKEVTHPG
                     IPARTFVGMREGDAPDFKLILAKHVEDAFATAAQEFNVA"
     gene            36164..36637
                     /locus_tag="PLB01_00315"
     CDS             36164..36637
                     /locus_tag="PLB01_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75768.1"
                     /translation="MSAIADIEADLVARLLDQIGALSPARGGTCRSVGDVRTATMLDT
                     PPPAVLVQYAGERAEIPAVIGPTRYRQMSRFQWDLKVIAASFTLDGEGREDQQERHGA
                     YTLLDSIFNALEGYRPPNSPHATRFFFQANSPYGLTDASVIYTSSWYCDVLRFKQ"
     gene            36811..38091
                     /locus_tag="PLB01_00320"
     CDS             36811..38091
                     /locus_tag="PLB01_00320"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HQQ75769.1"
                     /translation="MSDAQGSFEQWLVGVETTFKTPPGTPSGSKVPVINPQIKPSVNR
                     FYSKAFTGSSEPRPPVDGKIGVEGPVEVECSRESLGLFLKGVFGGTIVDSGTSAGYEH
                     ILTLGVQGSLFVEQGHTDISVYVLWRGMKVSSWTVNCDAEGLAQMTFQLMGAIQDDAT
                     TSSYFTGTITDRTGSSPLSYLDAVVKRDGVVVGGARKISFTLDRKLQKNIAIDGSSEC
                     RSITASDTPTLNGTAEFYFEDVNILNETLDGSNESSMEIFFPAEEAGCGLELHITTLK
                     LKPTGPVSQGQGIVTLPAEFDGYARGTASDIPARVSTKGFETVAVVNGSNDSLVISVS
                     GGADQTFDLGTTGNVSRSAIVTIVNATATGFLAVEKNDYIVFETTAKGAAVSIQVKAS
                     STCGTLLGLDNSLHNGQSAKSIYAVYRNLDATIS"
     gene            38170..38592
                     /locus_tag="PLB01_00325"
     CDS             38170..38592
                     /locus_tag="PLB01_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75770.1"
                     /translation="MKFFRASEGKGRWYTPFPDDFPDFKIEILRLSNEQIVTLADKWR
                     TSKPGTSSSEKYNFWRETCEQSVLNWEGAETLFKALFPDVTAFDCSKASKKVLMRSGV
                     IECDKVNRTTGEAYVERESLWQHLDHWTADQEAVELKN"
     gene            38844..39077
                     /locus_tag="PLB01_00330"
     CDS             38844..39077
                     /locus_tag="PLB01_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75771.1"
                     /translation="MAYRGMDGDPVGIPPTEVEAAARLNSLPWPGPGVVRFRIIKARW
                     FAYMVEQLKKRNAEAAARAKAPPVPEPRARRRR"
     gene            39399..43124
                     /locus_tag="PLB01_00335"
     CDS             39399..43124
                     /locus_tag="PLB01_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75772.1"
                     /translation="MAAGVGFMALSKGIHEAAEAESTIHGLEAAIRRTGAAAEEVGPR
                     IEKAIQTVFKTSAFDDEPLRRAAQLFVTITGNAEAAANAIGPIADLSAALGMDLESSA
                     RLVARATEGQVTGLARAGIIFTDTEKKMLSTADAGTRLAIVMGKINEKVGGAAAAELD
                     TYTGQVQELSRAWAEVAQTFGEIFLPAAKWVVSQLKEIADGIKSVIDAGRNNFANAPA
                     NGVAGGITNIEQIAAKGGRAGGRSIISDEDMARLDEAAKRMTAVQDAAGKIGLGQAAL
                     EAIGLARGLDQVNDQLNRLSQQQALEKMAPEIEAARQKVAAFAKDARDFDVKVQLALR
                     PPPDNEVEAAVREVEDKFDGLINGAKDKIEALKNDIATAKAQQTSLEVESRLAKMADQ
                     IDVAKVTSLNEQIELQDRIIAGATRALEKEQGIERSLERQAPAARQIALDDALAKAAA
                     KALAVYQKVEESVQKIGQEGKAALQGLVGAGGFGKTIDQAVSDAEGQLKLSFGFGPEF
                     RAGVQEAVQKMQKGIVDGAVAGAKEFDAKIRAAFDVSTARFQSAVENIGNVLGDIFTD
                     LFYTGGKNFGEIAGKMFQDAIKGGSQLLAKAIGGAAGNALGGSISRDQYASELEYQTA
                     VQNRTADTLNAAVSLTGLISQLASAGSGVNNPRQNPVGTAVGTTLAGASAGASIAGIV
                     GASTAAGGIIGAIVGLIIGGLMLALAPKVGDSYSYATMGVNKGKAFFDPTQNISDNET
                     KASLAKVQGTFDYFWSGYVDLLSKFPKMKLEGILKDMGTIGPTLGLDNPSGDIGHAAN
                     KDFWTQFNNWIEQGMPRDIAAKFVGPLQEGFVSLGLSAGKFQQIWSALDNIDPKAALE
                     ALNQLADALTTLGKTKDYFALSGGDLLARGRSDAAKSFTDQLGESDAEILRLGESLNE
                     LTGTEQIARLQQIAQLQDQRMQKEMEFIRSVNASIDSVNNSIDSRQRELDLALMKGPD
                     GKANTQAQIDYLKSYADSLLQKIGSAKSPDEVRALESESMSVLARITALGGNTDEYMK
                     WEKDQLETLRKVSVDSLTKMADEVAEQNAKFTDAIDGFIKAFQDATLTLTDLTGNGQN
                     PNNGGNIPTHTPNYSGGDEPTGNNAHGPWVFSDPNTPTHAGDFFDDYGMKPWERGYVP
                     PSQRNGDTGETNAPIAAPAVMEAFSEFTTSSRLQLEATQAQTAALNRMATAIEKGQHI
                     DLGTLTINTNGGSRMAPVVDTFAARQQIARAGARGNY"
     gene            43124..43870
                     /locus_tag="PLB01_00340"
     CDS             43124..43870
                     /locus_tag="PLB01_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75773.1"
                     /translation="MASIQRDIGTLTADQIQADPYKHYDFVSVDGGVDFGVVRYTNRE
                     RNWTGDLDGSSQTWTGGDNSGLQVERVSQSNTSILDVSSVSFLNVFDTDVAGHAVWGQ
                     REIQFGLKDREVVVYDAWWDPDDDTDAGFIDKFILFRGKIGATEHRLRSRLSLVPFRA
                     PGNIPTGRMIQDKVCSFALARLYGNTGEAAMTCNVDPGNTNGTLATFPTCPGTPAACI
                     ERDNYARFGGFPRMLKPNTTINWGNVKVPL"
     gene            43870..44505
                     /locus_tag="PLB01_00345"
     CDS             43870..44505
                     /locus_tag="PLB01_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75774.1"
                     /translation="MPATSTVLFPEGYAAVSFSEEPLPGQLAVGDGPDTGAEQVAERR
                     SRPLMRFSWRSEPLDVAGDAAYQAFVDQCGSGATNFTFYRPYSKFYTSVLVGYTDGTD
                     ALVVPFRNLNSWTSIKVDGSSDGGASVSNEFVSPTKEDKVITLTGPPAADLPITLENV
                     YGRQRFICRMLSWRRSLFQDGFTEALAFDPLSTTVPLMYAVYEFSFREKNG"
     gene            44498..48148
                     /locus_tag="PLB01_00350"
     CDS             44498..48148
                     /locus_tag="PLB01_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HQQ75775.1"
                     /translation="MADRGPVIRVEDNNETRTVDDRRLHYDEPPPWQDPTQNNPTVVD
                     DNSIVKRPIRRDMKGRTLVLNANGQIVPILYGKDQAGAKVPWGEAHGPYLYMMFVVGE
                     GPLEAIEDIGFGDAIETSFARLGLVAGTDYNVHLGDPAQAVDSIFSAIDSGWIWVFPR
                     LAYVTVKLNINTPNMDQIDVGRFRCNVKGRQVVDSRIDPTLADASRAYSDNPALCELD
                     FLTSRLFGMGAKLSEVNIDSSNEAADDCDVVTASGLTAPTGTPTDTVNSGTGTIEAGI
                     YRHAYTFVDTNKVESLLSPPSTEVTVISGLSQHITVSGLDAPGGGVFRNLYRQKKNPD
                     GTTWGPWKRCEILEDDSSTTFNDFLPTRLLGGSPPDETDIRQFRLSMVVDQAGETCAQ
                     TIDTMRQCFASQLAFNNGKFQAFVDKVRDPTTIVFGDHNIVDLPTIEGRDPSQIPAQV
                     VVNFTDAANSFAPRQAFYPAAPVIGEGDSRSDRNILSFDLPWIPTFDQAMRIAIWIWK
                     RLNRSISLSWRTLQVGALPIPGSIVTLTHARAGIPETQVIVTSCAPTADRVYWDLTGE
                     IYDPDDYDLDYHSEDDGAPPTHEPPDPPDPPTNLVLTNVPRTGGNGLPTVDVKIEWDS
                     AEMPYETSTLVRFSTDGFSTFTELGPFVTGPVFLYNPTLGIQYDFKLYTVRVSNGQLS
                     ADSLDGSIVPSFTDTSIPEVTQIQIGPNNGLTFYGPTPEYFITTSNESMPPIPAPTLA
                     AAAGGLVDDGDHLVGSTRVFSWGETKLGATALVTLGGGNNRINITNLDPSPPAGTLFK
                     KVYVTKVGPGADFFHASDDAPAGTSDSLIHADSSYVTNDLLPEECHGLVGWEIFDAFG
                     VGDPTTDPMPLFKFLPWNKGPQRYAALDLSPIVHEDPTDHAPRIDVYIRVVVVEEGGG
                     AANSTGVLFSKHPATWPPTGPPSSIPVHVTVVDGINEDLDIPIDANFVYLDDGGLTAD
                     FILGGLTVAGSTPTTRKRIVLVNNTPFRANLGHESAGSTAANRFAVPGANFYDFGPVL
                     GSTTIEYGVDESDRWTPIAINNAGQGPVTDTDGGGGTFIRNSLTEQPESDISISGLAH
                     ARQFAGNVDFSGSVVEGGTYDDLVLDVWADVVHLPACTITGIIPPDQGLAVDGVTPNK
                     FGVIVTLVNASATGVGNGIEFKHNDAGSAAANRLAGIDDLDMATGAVFRQAFTIQYDY
                     TNSFWIVVRRNF"
     gene            48148..48453
                     /locus_tag="PLB01_00355"
     CDS             48148..48453
                     /locus_tag="PLB01_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75776.1"
                     /translation="MKRLCLLPVLLLIALGCATAAQKRRNAWAQARNTYVAAKAVYDG
                     ACRVKPHTRDDCADFYSELAASYDALEAAAPAVQAPAPIQMAAVEKAEKALEARVAK"
     gene            48450..48635
                     /locus_tag="PLB01_00360"
     CDS             48450..48635
                     /locus_tag="PLB01_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75777.1"
                     /translation="MTAEVKVAGPYDPFPALAELMKLANSPAGQAWLERRLEDAGISQ
                     AELEAKAKELQPPEQPA"
     gene            48677..49123
                     /locus_tag="PLB01_00365"
     CDS             48677..49123
                     /locus_tag="PLB01_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75778.1"
                     /translation="MRRILGSLKLVAVAAAIALLLAPAFAGAAPLSPILASPTIHAQI
                     FGGAVPEGVLAVVVKALAIGVLIWKGIDGLKDLFPDWTAAHPGALRIFNAVAALLASL
                     GVCWATGMAHTWVEVGWCSIQGVLAFITAAGLHEGKSASDKARAGR"
     gene            49219..51699
                     /locus_tag="PLB01_00370"
     CDS             49219..51699
                     /locus_tag="PLB01_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75779.1"
                     /translation="MKKAPLLALVALLAASAASAGDFFASPTGLDTNDGSLAHPWTIT
                     KALCSDALGNAQQCKWTPHAGDTIWLLDGQYDGLFNGNIPGSATAQTWIRGWSRCPVY
                     GSAGHCGAIINGGILQNPSTFRFGQSYLGIRDVEITSEPVRRQTEDPGWGGNESWPRD
                     VELADGINSDATLQLTGNKIVNVIVHDTRQALSLWSQMGDKEAYGAILFYSGWKGLTR
                     GHGHNIYTQNNLGEEWIENVYAGYGFQEMVQAYGSSVASLNNFRLWRNILHSASSLGD
                     LGGRNLTLGGTADLNNAELIGNSIYCGPLFDCGTSDLYVGYNSNCVNLKINGNTVWGS
                     WLFGKGTGCTTPAEMTGNFVGPNPTAVSPAVWDASKYPNNTFAKGIKPANPIVNVYPN
                     KYEQGRAHVAIFNFPQQPTVAVDLGDVLNVGQEFEVRNLEDVFNSPILMGTWTGAPVN
                     FPAGKMSTAQPKGFLHTIDPQPTVGPGTPTPVPTEHPYPVPAPPGTGPEFNAYLVRAK
                     IPGTFVPSKTPTPGGSTPTPSATPAPSVTPTPALLWNQELPVAAAVIVAPVANNGGVL
                     STSVDGQTVIPAGTASWTFTVANATTVRVWANVWTGDAQSDSWHTRMDGGPWATFDSS
                     LDSGWPAPPVWNWVKVNARAPFENPRLFAVAAGQHTLTFGGREIGSLLRDVYVTNDPS
                     FMPPPFVPPAATATPTATMTDTPTATATRTATWTFTATSTATPTASPSPTSSPSATPS
                     PTPTSTSTRTATATATPSFTSTPTLTATATPTATKAPPTVTPTATKTATSVPPSKTPT
                     RTPTWTNQVRDQHIRALEIVVYTATPTP"
     gene            51696..52379
                     /locus_tag="PLB01_00375"
     CDS             51696..52379
                     /locus_tag="PLB01_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75780.1"
                     /translation="MSVRFDTIADYLDNQDSPALPAALAPVSFSMWFYVTSMPAGVNA
                     LVFNFFADNLDAACDIYVRESDKMVQANNGVGGGAVGHDIATIALGTWHCAQISKSSS
                     QFNVYLDGAKFGFTETARTVNPPRNAHIGGYGGGSRLNVGYVTYARTWTVALTDDELA
                     AERCSAVPVRTSGLYGAYPLATNANDASGNANHWTSHGSPVYSAFEPTLPNLAPIPGR
                     VRRAQFVYG"
     gene            52389..>53001
                     /locus_tag="PLB01_00380"
     CDS             52389..>53001
                     /locus_tag="PLB01_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75781.1"
                     /translation="MLNLVGTSAKLRVTTSAPGTIAIHCQASWMDNLNGGITPGATNT
                     ASITSATTTDIVASPATAGTTRNVKGLSIANAHATNQADVDVIHTDGTNALTIFQATL
                     APGEVVIYSDLTGWVHFNSAGTPLNSQSVAGSDAQSYTLTSQSPWTKPTSFTPKFVAV
                     WGWGAGGGGGAGASLATATIAKGGAGGGGGAYNLKIFLASELGA"
BASE COUNT         9265 a        18283 c        17458 g         7895 t
ORIGIN      
        1 gcgcgatctc gggggtcgcg agcggtggcg gcggtggcgg cgggacgggc ggcaccggcg
       61 gcgtcggcac gaccgccgtc ggcaccggag gaaatccggt ctcggccgcg aacacgacgg
      121 tcaccggagt ctgtggcggc cagggttctg ccggcaccgt gacggtcgtg accacccact
      181 gcgccgaatg gggcggtggc ggcggtggcg gatcgacggc tactcctacg tcctcggtcg
      241 ggggttcctc gctcaagggc ggcggcggcg gcgggaccgg aggccaccac aacgcgactc
      301 cggcgatcgt cgcgggagcc gcgggcggcg cgagcgaatc ctacacgggc ggcggcggcg
      361 gcgcggtggg aactaatggc gcctcgccca cggccggatc caacggctcg ggcggcgatt
      421 ccacgaaggg cggatccggt ggcggcggtg gcggcacgac cgtcacggcc tcgaccacgg
      481 cgggcgccgg aggcaacggc ggggacaacg gcgggggagg cggcggcggt ggctgcggca
      541 tgaatcccgg tctcggtggc gcgggcggta cgggtggccg cggcgccctg tatgtcgtga
      601 cgtggtgatc tgatccgtgg ctggccccct cggatggttc gaccccgaac tacgcgatac
      661 agcgcagttc gacaccgacg ccgtcggaga gggatccttt gaccgggatg gtgtagagga
      721 ctcccccccg attccgccgg cggcggtctc gaggggggtc atgcaatcgc tcggagcctt
      781 cccccaggct gcgaccgcgc tgatcgtcgc gctccaactt gggcgcacgg tcgtccccgt
      841 ccaggcggtg ccttacgacc cgatcctgac cggcaagccg cgggagcaga tcgcggcgac
      901 ggcgccatat gtccggtatc accccagggt cgagacagaa ctcgcgcgcc gccaaaatct
      961 tcgacccata gtcgcggacg ccggaggctt cctcgggggg atcgagtggg gcttggcggt
     1021 gttgccgcgg ccccaacacc ccgccgaggc ccaggcggtc gtccagtcga tgcggggcct
     1081 cccgcccccg tcgacagtcg cctacgatcc cctgacctcc ggcaagcgcc aacaaggaag
     1141 cccgccgtct ccgccgtacc cgcaggtcga aaccgccgcg gccgtcgctc aggcgaagcg
     1201 gccgatcccc ttcgcgctcg cctacgaccc tctgaccctc gggcagcagc aggatccggc
     1261 cccggcacct ctgccgttcc cgcagaccga gacccagtac gccgcaggcg cctggaggcg
     1321 tctcccggtt ccccaggatg ccggacagtt cctcggaggc atcgagtggg gcctgatggt
     1381 cctgccgggg gaccggtcag tctacggcct cccggtggcg cagagaaccc tcccggtgcc
     1441 tccggcaccc gtcgcggcgt tcgacccgac gaccctctcg aggcccgccc aggccccgcc
     1501 agaggcctac tacccccgag cggaggcggc cctcgccaac gcgcgtccga gcgtcagcac
     1561 cgacctcgat gtcctgacgg cgggccgacg gcagcagccg ccgccgcccg cggcctacta
     1621 cccccaagtc gagacggcaa cggtcatcgc tcgagccgcg cttccggtgc ctttcgctcc
     1681 gacctacgac ctgttcgcct ccggcgagcg ccaggaatcg ccaccgccgc ccccctacta
     1741 cccgctcgtc gaggtcaacg gactggccgt ccagcttgcg aagcggccga tcccgttcgc
     1801 ccttcagtac gaccccctga ccctcggcgt ccagcaggat ccggagcccc cgcggctccc
     1861 gttcccgcag accgagaccc agtacgcggc gagcgcctgg aggcgcctgc cggtcccgtt
     1921 cgcgccccag ttcgatccgc tgacctcggg aaggcgccag accccgccgt ggctcgagcg
     1981 gtatcacccc agggtcgagg ccgcgaccgc gctcatcccc ggcacccgga cggtcatcgc
     2041 gcccgagttc gaccccctca cctcgggccg gcgtcagggc tccccgcagc ccccgcccta
     2101 ctaccccgac atcgagacgc ggctcgtcat ccggctcaac cgtcagccga tccccttcgc
     2161 cccggtctac gacccgctga cctccggcaa gcggcaggcc gcgccctgga tcgtccccta
     2221 ctaccccgcg gtcgagctcg cgctcgcggc ctccgagaag gctccggttg cgccggtcta
     2281 cgacagcctg acctcgggca agcggcaggc cccgccgccg ccgcccccgt acttccccca
     2341 ggtcgagagc caggaggcca accggcgccg cgtcctgccg atcctcgaga ccgacattct
     2401 ccggtctgga cgccggatct cgccggcccc gctgccgccg ccctactacc ccgcgaccga
     2461 gacggccctg gtggtcagcc tcaacgcgcg gccgattccg cccgagaact tcgatgtcct
     2521 gacctcgggc cgacgccagg atccgccctg gctcgagcgg tacgcggcgc gcgtcgagac
     2581 cggcctggtc gtctcgctgc ggcttcgcgc gccggttccc gagcccccgc tcgacacgat
     2641 catctcgggc ggccgtcagc cgaatccgca gcccccggtc tactaccccg cgatcgagtt
     2701 ggcctacgcg aaccggcggc cgctgtccgc gctcgacgtg acggtcggcg tccccggctt
     2761 catcccgccc ttcggcaccg cggtcgatcc tgaccggggc caggccgatg tcctcgaagg
     2821 ctacggcgcg tcggtcgagt cctcgcgccg cggcatcacc gtctacggcg gattcggaac
     2881 gagcgttgaa tcctcaagga gaaagacctg atgcgcgaac acttcatggg gcagggcgat
     2941 ctcctgccgg aaatctacgc ggacctcacg gacggcaacg ggaacccgct cgacctgacc
     3001 acggccaccg cggtcctgtt ctacatggac ctcgagaccg gcgcccacaa gatcgacggc
     3061 gttcctgggg ccgtcgtggg cgacccgacc ctcggccgcg tccggtacgt ctggaccggc
     3121 accgacacgg acacctcggg cgttcaccgc gcgcagttcc gggtcacgtt cccgacggcg
     3181 ctcccggcga gtttcccaaa cgacgactac atctacgtct ggatcacccc gaaggtcggc
     3241 tcttagagcc agtacgggcc agaggcccgc aggagagtcg atgctttggc gacggcgaaa
     3301 cggaaatcgg ccagtcggcc gcgaaacggg agagttctca cgcgaagcga tgtcgtggag
     3361 gcggtcgagc acgttcggga tgaggtgatc ctccggatcg acgcctcgga caagatcacg
     3421 gaccagaaga tcgggcgggt cgcggacaag gtcgatgcgg tcgagggcaa ggtcacggag
     3481 gctaagcaga tcgccgccac cgggatcgcc gagacccaca tcgcgatcgg caacgtcaag
     3541 ggccaggtcg agaaggtcga cgcgaaagtc tcgacggtcc agctcacggt cgcgaagatg
     3601 atcgggtggg cggcgggagc cggcgcggtc gctggcgcgg tcgtcgggat cgtcgtgaag
     3661 ctgctctccc actcgtgagg ttcgcctccc cacggatcgc cgccgagtgg cagggcgcgg
     3721 acctcaaggg cgtccgcgtt gtgcctgcgc tcatcggcat agtcgaggac gcggccgact
     3781 tcgttcggtc gctctacgcc tgggagctga tgctgacctg tctcctccgc accccggagg
     3841 agaacgacgc gctctatgtc gggcgcggct atcacccagg gacgcacctg gacggcgtcc
     3901 acgttcgggg ccgcggcggt gacgcccgca tccgcgatgt cgacccggag gccgtcaggg
     3961 gcctccacgg ctacttgaac gaacgctaca tctacgaccc ggagcggccg acgctgtcgg
     4021 tggcgctcct cgaggacggg atcaccgctg gctcgggcgc gcacctgcat ctccaggttc
     4081 atccgcgcac cacgaaccgg catttcacac aggagccttg atgccccgaa agcgcgtgga
     4141 ctgtcccggt ggctgcggaa agacgatccg atgcgactcg aaactctgct tcgactgtga
     4201 gaacgtgcgg cgcggaaact cccggcacgg ggcgccaccg ccggcggcct tcgagccgat
     4261 ggctcatcct ggcgaggtga agtggatccc gggcaagcgg atgcagtgcc cgatctgcct
     4321 gcgcgacttc acgcagaccg agccgaatca ggagactcac aaggcctgcg cgggtcgcgt
     4381 caatcgcggg atcgcgagca acccgaagga actctcgaca ccgtacaggg acgccggcta
     4441 tacgccaggg aagcagatcc cgaagtggaa ggccgagccg accctcaagt cgccaatgcg
     4501 gatcgcggtc ctggacctgg agacattcgc gctcgatcgc gggtgggggg tcctcctcgt
     4561 cggcgtgatc gtcgttttcg gtgatggcga gccgaaggaa tatcggttcc ggctcgacaa
     4621 gttcgacact tacaagcgcg accgctctga tgattcggag ctcgcggcat cggtgttgtc
     4681 ggttctcgag gattgccaca tctggtatgg gcacaactcg aaatggttcg acatccccta
     4741 cctcaactcg atcgcgctca agtacgggat gccgccgatc gagaggaagc tacgcgatcc
     4801 ggttcaacta ctccgggcga agttccggat cggggcgaac tcgctcgagg cggcgaccga
     4861 cttcctgggt ctggaacaat cgaagatgca cgtcccggcc gaggtgtggc ggcaggccgc
     4921 gttcaacgga tcgaaggccc atttcgacat cctcgaggag cggtgtgttt cggacgtcca
     4981 tctgctcatt gaactcgcgc ggcgcttgga ggactggggc ggcgtggtgg attactcggg
     5041 attttacaga cgatgaacct caaggagttg gcctgggcgg cggggttcta tgacggcgag
     5101 ggctcgacct gcggtggcgt gaggccggat cgagcgcgcg ggacgcggct gatgatgtcg
     5161 atcacgcaga ccgatccgcg cgtcctcaag cggttccaag cggccgtcgg cgtggggcgg
     5221 gtgaacggcc cctacgactt caagggtaac cggcaccgcc aggcgccaca gtgggcgtgg
     5281 cagtgccaga actggcgaga cgtccaagtc gccgtcgcga agttgtggcg attcctcggg
     5341 ccggtgaagc gcgagcagat ccagcgagcg gtcgcggcct cccgaatcgc agcgcgcagc
     5401 gaggcgcgtc tcgcagtctc cctgtacgcc gaatacaacc gtggttggga tgagtgccac
     5461 ggaacagttc cgcccgccga ggagaagaag tgaacatgga caagtgtagt taccccgaag
     5521 ggttcgagga cttcatcaac gcccgctacg ccgagccgtg gttcaacgag ccgcataagt
     5581 ttacgcacgt gcgcgggatc gaactggcac ggctggcgtg gcttgccgca accgacaggg
     5641 agcgcaggcg gtgcgttgag gtgacgtgga gcgtgaggtg ccgtggtgag cattgtgact
     5701 gttacgacgc aatcagaaag gaaatcaacc aatgactacg ggagccggag acgatgggtt
     5761 ctacctcgcc gttggccgaa tcgtccactt caacgacaac gattcgacgt gtcaggcggc
     5821 aatcgtgacg ctggttcgtc cgggggattt cgagccggtc gatctgactg tgttccctaa
     5881 cggtggcgga gcgtcccatc agggcaacgt gatccacgtc gatcaacccc ggtcgtggca
     5941 ttggccgcgg ctctgccaga cgtaccggca caagaccgga agatcgtccc ccgtgcagcc
     6001 cgcgcagaag gacgcggctt gtccttacgg gatcacggac tgcggatgca cgaagccgca
     6061 tagcaacact caatggtgcc acggctgtcg gcattggtgc gtcggtgagt attgcgcgag
     6121 aatatgtccg cacccacccg gcaccacctc ccccgcgaag gcggaatccg gcaaggactc
     6181 caagacgcaa ccttctgagc ggcggtgccc cgattgcggt ggaccagaaa gcccgctacc
     6241 tgacgacctg agacgaatta gctgcatcga agagtcgccg acggtctaca ccgacgagat
     6301 gatctcccag cggcgcgccg agatcctcgc gacgagcttt gcgatcgcgg gaatggggac
     6361 tcccggtcgg acatccgccg gcggcgtcag catgagcggg aggatcctca cccgcgagga
     6421 atacgcggac gcgatcgaca tggtccacgc gaccgagaag cgcgaactcc tcgagcggtt
     6481 cgacgtgggc gcggagcgtt acgggatcct ggatctctcg ctcaaggact gggagcgcga
     6541 ggaacacgag gagcgcctgg ataaaaaggc ctacaagatc ttcgactggg ttcggaaggc
     6601 tcgcggcctc tgatgggagt gatcccctac agcttcacgc tatggggcgc cgagtggcgc
     6661 gtggtgtggg gctggaccgg cccgcagcac tggcggggct tccggtggaa cccgaagcgg
     6721 ctcgccggcc tctgcgtgtt cgagccgctc aaggggaacc ggatcatcat cctcaacccg
     6781 acgctcaagt cggggagcgc gatcaagcgg tgggaggcct tcgcgcatga ggtcctccac
     6841 gcgatcacct acgaggccag gttccggcgc gtgtcgcatt ggaaggggcg ggacctgtcg
     6901 cactcggtca ttgaccggct cgagttcccg ctggcggtgt tcctgatgga gaacgcgcga
     6961 ttcggaggag aaaacgaaag ggccgggagt tgattccggc cccctgcctt gagtggcaga
     7021 atgtttgaac ggttcgattc taccccttct tcggccaccg cttcttccgg ttcttcgcca
     7081 gcgccttgag gcgggcggcg ctcggggtct tgcctccgcg ctcgccccgg caggcgggac
     7141 agatcatcgt gacgtccttc ttgtggcgag tgcagatcgg ctcctgccga ccctccggat
     7201 ggaacacgat tcggacgcgc gggccgagcg agcgggggat gtcggggtct tgatcgttca
     7261 tccacgaccc ccgttgttcg tgcgctcggt cttgaggcgg gcctcggctt cctcggcgag
     7321 gcagctccac gaacagaatc ggtagacctc ggccgaggag gtcccttcca gcgggatcag
     7381 gagcgtcagg gtctcgcgcg tgtcgtcgtc gacttggatc tcgtccgagc agttgtcgca
     7441 gccgtagtgc gtgttcatcg tcatcgcgtc cactccccga cgatgcggtc cctcgggaac
     7501 gctgacgccg tgttccccgt catccgacgg gccgaagttg acgtggcgga ggcggacgta
     7561 gcgggcgtcg gggaggccct tcttcgcgcg gtattcgttc gcgacagggt ttcggggccg
     7621 agcaggaaga accgctcgcc gctcgcgagg acgacgatgg gcgggtcgta ggtcagggct
     7681 ttcttgttca tcgcgcaccc atgagttcag ccatcgcctc ggtcgacgtt cccttcggga
     7741 tgcgcccgaa tgtgcgctgg tagacgaccg cgatcgccca gtgggcggcg ttgcgggcgc
     7801 aggcgcgcga gaatttccag tccttccgga gttccatgct gcggagcggg gcggtcgcga
     7861 ggcgggcgac gagcgagtcc atcggtccct ccgagaaaag gaaagcggga ccgttgccgg
     7921 tcccgcccgt agcgcggaag cgcatcctcg caggttcgcc cgagagggta cgctcgctcg
     7981 ctccggaagt caaagagcgg gaccgggggg actcgaaccc ccaacctgca acctgcgagg
     8041 gtgctgcgct atccgttgcg ccacggtccc gtttgctgtt cacgggagtc atgttatcaa
     8101 aagcagcttc tgagtcaagc gaaaagggaa agaaagggtg gcccgtgggt ggccctttga
     8161 aagccgggag ccgacccctt tcagagcctc ccgtaagtct cgcatttcag ggggtttatg
     8221 gagccgatga ccggacttga accggtgacc tgcggtttac gaaaactcag ttcgggcgct
     8281 ctggatcccc cgaaatgcgg attcccgagg gttttcgggc cttcctgccg gtgcctgggt
     8341 ggcctccggt gcggccgtgg gtggccccag ggtggcccga ggcccgccgt ttccgctgcg
     8401 gttttccggg tggcccgctc atcggctcga gcgccgccgg gaagatatgg gcgtaggtgc
     8461 gcgcgaccat ctgcggcgtc ttgtggccgc accgcgccgc gacctcgggg atcgaagcgc
     8521 cagcgtagag cgcgttcgtg acggccgagt ggcgaagcgc gtggggcgtc attcccgccg
     8581 gcgcgatctt cgaccagaac cgtcggaagt tgtcgcggtg gccctcgccg ccgcgccctg
     8641 ggaagaagta ggggccgcgc tcggggatca gctcgaggag ttccttgagt cgcccgacga
     8701 tccggaagtg ccgcgcgttg ccgcccttct gcggcgggaa ccggaggaac tccgggccga
     8761 cccacgcgcg ctcgaggccg tggatctggc tgatcctcga gaaggtctcg taggcgagct
     8821 cgatcatcgc ggcgttgtcg cgccaggcgt cctcgaggtg ggccggggcc tcccgcgcct
     8881 tcgcccaggc tcgcgcgatc agggcggcga cagcctcggg ccgctgcgcc agggcgaggc
     8941 gccgcggggc tggccgtagg cggcgcacct cgcggcaggg gtcggcctgg aacccggact
     9001 cgcgcgatcg ctcggccgcg aaccgcagcg cggagcagag aaccgagagg tcattgttga
     9061 cggtctgcgg cttggccttc gcgaggcggg cgtcggcgta agcccggata gcgtcaggcg
     9121 tgatgcgatc gagcggggtc gcggagccga ggccctcgag caggacggtc caccgccggc
     9181 ggcgggagtg gccctgggcg aggccagcga catccctgtc ccgtagagcc tcgagcgtcc
     9241 atgaggccgc caggcgcgcg gagacgggca ggccgacccg ttcctcccac tcagccttct
     9301 cgagctgccc cacgagcctc cgggccagct tcgcgccttt cgggccggag ccgcaggatc
     9361 gctcgcggcc ctgccggtca cggacgaggt agaaagtccg gccgttgcgc cgccgggagc
     9421 ggatccacgc cacctacttc gcggcggtct ggatcggggc cttgcagagg tagatgtcgc
     9481 ccgtcttgga gccgacgaga agcgtgtccc cgccctcgcg cacggtccag tagcgggcgc
     9541 aatccatcgg agaaggggtc tggccgccgc cgacgatcat ggtcgcgcag ggggccgagt
     9601 tgatgtcgat cccctcaatc cgccggcacg aggaaacgga actcgggttg gaggtcacgt
     9661 tgactctgta gcgcagggca taggtggcct cggtctggtt cgctgatgca cagccgaggg
     9721 tcgcgaccgc gaacagaatt ccgagaatcg ttttcatggt ggtctccctt tttgaacgga
     9781 actccaccac tactttttcc ggtggtgggc ttttgctcat cttttcgcgg ctctcgccct
     9841 gcccccttgc ggtgccgcgt gtcggtttcg cacggttcct cctggtggcg gcgactcggc
     9901 cgcagacctg cgctcggcgc gctcgaggaa ctcctccagg gcgatgcgaa ttacggaact
     9961 gcgggcgcgg tccccgtccg ccttcgcata ggcgatgagc cgcgccgtga ggtccggcgg
    10021 caacttcacc gaaatcggtg gtgccacgat gcgtaaaggg tagtacccag taccccccga
    10081 tactagaccg cccgacttac gcctgcggca attttcatgc ctgacttgag ggtaaatccg
    10141 ggtagcgtcc ggtagcgtcc ggtcgtctcc ctttttaccg ttgactacag ggtattaccg
    10201 ggggatactc cgtagcgtgg accaggaaat gacaaagccc cgccgccgca tgaagccgat
    10261 tccggcgaag gcctgggcga gcacgatcac gaaggcacag cgcaacgcgc gcaagcgcaa
    10321 ctcgacgctg tccgctctcg tgcgccggtt cgtcgaggaa ctcgccagag aggacgccgc
    10381 cgcatgatcg acgaagaact cgacgacccc tgcgacggcg gacgctgcca ccgcgaggag
    10441 tccggaacgt ggctcccgtg ccgtaagcac gagcagcacg accgcgacga cgaggccgag
    10501 tcgtgaaggt tggaggtgcg ccatgcgcga ctgactcgcg gggcgtcgtg gtcggtgcct
    10561 tcgtgacggt tcccgaggcc gctcgccaga cgggcttctc ccgctggacg atcctccggg
    10621 ccgtgcgcga cgggcgcatt cgggcggtgc aactcggccg cgccatccgg atcgcgaagc
    10681 ccgacctcga cgccgcggtg cgcgagtggg agcgcagcgc ggtcgccgtc tccgttcacg
    10741 cccccaccta caccgacgcc gtcatcgacc gagttttcaa tcgctcgcgg gcagcgagca
    10801 gaaagggaac gcgatgagtc gctcatggag agatcgaagg agatacgagc cggagccacg
    10861 ggactcgcag ccgaccgtcg actgtgtcgg atgcgggcgc gagttttcac cctcggacga
    10921 ggagttcccc gcggtgatgc ggggcgaggc ctacatctgc gaggaatgcc tgatcgcgca
    10981 gccgaagcgg gtggcgcgat gaaggcctcc accatcgaga ccgtcgccgc catcggcgtc
    11041 cgggttctgg tcgtgatcgg agtcatggtt ctcatcgcgc ttgcgaccct cgcggccgtg
    11101 aagatggggg gcttccgatg aaacccttcg agcgcaacgt cgcggggttc atcttctcgc
    11161 tcctgttcgg cgcggccctg acgatcctcg tgggggtggc gctgtgacca tcatctgcgc
    11221 ctcctgcaag aagccctgcg agggccacaa gcacacgaac gcggggtggc tctgccgctc
    11281 ctgcgttctc tcggcctcga ccgtcctcgc cctctacatc atcgacggcg tcacctacgt
    11341 cgagaagctc aagaccccgg ccgtcctcgc agcggttccg gtcgaacgcg agcgccaagc
    11401 ggtggcgcga tgaaccggga cgagcaactc gacgcgatcc gcgcgtggtt ctgcgcgaga
    11461 caactccacc acgtcaccaa cgcgaccatt actcgtcggg tcaccgtggt caacaccatc
    11521 tgttctgcga tctcggccta tctcggcgcc caccgtggag gtgcgcgatg agcgacaccg
    11581 cgctcgtgac ccagtccgac ctccacgccg acgcgctcgc ccgaatcaat gagaccgtcg
    11641 gctcgctgcg cgccctccgc accttcatca aggcggaaat ggtcaagggc gtcgactacg
    11701 gcgagatccc cggcacaaag aagaaggtca agaccgagga cggcaagacg gtcgaagtcc
    11761 ccgcccaggc ccttctcctc gccggcgcgc aaaagaccac catgtatttc aacgcgcgtc
    11821 cccggctcga cgtgcgcggc accgagctcg ccgggggcca tgtcgaatac ctcgtcgtct
    11881 gcgacctgat ccaccgcggg accggcaacg tgatcgcgca ggggatcggc tcctgctcga
    11941 cgatggagag caagtaccgg ttccggaatg cctcgcgcgt ctgccccgga tgcggccagg
    12001 ccaccatctt caagtcgaag gagcccaagc ccggaaaggg ttggttctgt tggcgcaaca
    12061 gggggggctg cggcctcgag ttcgacggcg ccgcccccga gatcgtgaag caggacaacg
    12121 ggaaggtcga gaacgagaac attcacgacc agcgcaacac cgtcctcaag atggcgaaga
    12181 agcgcgccca ggtcgacgcc gcgatggggc tcggctgcat gagcgaactg ttcactcagg
    12241 atgtcgagga cttcgacacc tacgagatcc ccgacgaggg cttcgcggcg ggggagatcc
    12301 ccctcgacgc cccgccgccg gcggcgaatg aagttccgaa ggccgcgccg cccgccggcg
    12361 cgaagcggcc gtggctcgag gtcatggccg agcagaaggg ccgcgtcggc gccgaggcct
    12421 acctcaaggt tctcaacgac tctgagttcg accgcgcgga gcagatcacc gaggtcgccg
    12481 accgcgcccg catctacaag gagctcctcg cgatccccgg cgtccagcag ccagagaggg
    12541 cgaagtagat ggagctcgtc cttacgcccg accaccgcta catcctcaac gggcggcaca
    12601 tcccggggac gaccgagccg ctcgtcggcc tgggtctact cgacacccgc tacttcaccg
    12661 agttcgcccg cgagcgcggc cgcgcgatcc acgccgcgat ccactaccac ctgaccggag
    12721 gcgtcgactg ggcgacggtc gatccccggt tcgtgaagta cgtcgaggcc gccctacggt
    12781 tcatcgacga cgccaacttc acgccccacg gcgtcgaggt cgtgaaggcc tccgagcact
    12841 acgggttcgg gggaacgctc gacacctacg ggatcggctt cggcgagttg atcctgcccg
    12901 actggaagtc gggattcctc ggcgagatca ccggactcca actcgccgcc tacgagctcc
    12961 tgctcggcgt cgaggcgcgg cgcatggccg tccagcttcg gcccgacggc acctacaaga
    13021 aaaccgactt caccaagcgg caggacaggc cgcggttcct gggggcgctc gacctctatc
    13081 ggactttcat tttcaaagaa ggagaagaac gtgttgcagc atgaaccgat cgaagttccg
    13141 gacgcgggtc cgatgaagca ggagggcctc gcgatcgtcg agcaagccct cgcggtccac
    13201 atcaccgacc ccgaatcgca caaggccgcc gccgaacagc tcgtccacat cgcggggatg
    13261 gtcaagcggg tcaaggccgc gttcgccgaa cccaagcggc tcgcccacgc cgcccacaag
    13321 tccatcaccg cgctcgaggg cgaggcgctc gagcacctgg agaaggccga ccggctctac
    13381 cgctcggcga tctcccgctg gctcgtcgag gaggaccgcc ggcgcgcggc cgagcaggct
    13441 cgcatcgagg ccgaggaaag ggcgaaggcg caggacgccg cgatcgccga ggccgtcgcg
    13501 ctcgaggccg ccggcgacat caagggcgcc gagcgcgtcc tcgccgagcc cgtcctcgcg
    13561 cccgtcgtgg agatccccaa aacggtcacc ccgggcgtca gccagggccg aaagagctac
    13621 gacttccgca tcaccgaccc cgccgcgatc aagcgtcagt acctcatccc cgacgaggtc
    13681 gcgatccgga agatcgtgaa ggcgatgggg ccggacgcgg ccacagtgat cggcgggatc
    13741 gaagtcctcg aggagcggat cgtctcggtg cggggcaact gattcggatg caaacaccgg
    13801 ccggagagcg ggccctcgga cttcgccgcc tccggccttt ttcttcgccg cgcaccgtcg
    13861 cggccatgac agcgggggtg gagcggatgg caaagacgat cacggtgggg ccgctctgga
    13921 tcgtcccggc gatcgacatc gaccgggagg agcgcgatca ggcgctcgag cggcaagcga
    13981 aagcgatccg acggtcggcc gctcgcgccg ctgcgcgaag ggccaggcgg gtcgcgcgat
    14041 gaggatgttc cgtttcagtc aatggcgccc ctacggccac gactggaagt cgtggatgtc
    14101 gtggttccgg cgctcgagtg ggagctacgc ggaggtccgc cgctgcgggt cgttcacggt
    14161 gccgccttcg gagcgccacc gccacgcgcc gcgagaggaa ggacacaccg cgatctatgt
    14221 gctcggcgag gacgccttcg acctcaatcc ggtcgagggc ctggaggtgg aggatgtgga
    14281 tggttcgacg gttcctctcg gcaacggctg agttgctcgt ggtcgcggtt ctgctcgcgg
    14341 tgtggttcgg gtggctcgcg ccctcgcggc gcaacgcggc cgtcgccccg gctccgcggg
    14401 tcgaggcgac ctacgagaac acctgcaaga tcgagaagtt cgacgacgag gccggcccga
    14461 agtggaagtg ctaccaggac ggcgtcaagg ttccctgccc gcgcgccctt ccgaaactcc
    14521 gcaactgcaa cgcgaccgag gacgtgatcc ggtgagcggg cgcgagttgt tcgtgaagat
    14581 cgtttcgctc tgctcggttg cgatcctgtt cctgctcctg ttcttcgcgg tggccgtcgt
    14641 cctcatcttc ggggtcggct cgtgcgtggg ccagcccctt ccggagatca agttcaccga
    14701 ggccccgtac tcccacaccg gcttcgcgac gagcggctgc gtctacagcg gccagacctg
    14761 cgtccacgtt caacagtggg gcgcctacgc gctcaacgtg gtcacgggcg gattcaagat
    14821 ggtctcgccc atcgacaagg gcgggcagtg cgtccaggcc ccgaagttca tcgtcgccgg
    14881 cgactgtcat cagagcggcg tcacggtcgc cgcctctccc ggcgcggaga agtgggtcgt
    14941 cgtgacgggc gctccgaatg accagtgcaa ccccgaccac gggcagttcc agatcctacg
    15001 gctcgacggg gcctcggggc tgcggtgggt aggcgaggcc ttcgcgccgg cggtgggtcg
    15061 ggtcaacacc gcgccctcga tcgacggcga cttcgcctac gccgtcgagg gcaacgggca
    15121 gaccttcacg cgcgtcgacc tcaacaccct cgcggtgacc tcgggcctcg gctcgaggcc
    15181 gccctgggtc ccgcagccga acgtcggcgg gtacacctac gcgatcaccg tcgacccggg
    15241 cttcccgcag gtctgcgggg cgccgcagca cttcatcgcg acccggagcg gctcaacgcc
    15301 gcctcccacg ccaccgccta cgccaactcc gggcggcaac ccgttcgcga cctacatcga
    15361 ggcgctcgcg cgggccggtg tgaccgctgg ctgcggcggc ggcaacttct gccccgagcg
    15421 caccatgacg cgccaggaaa tggcgacctg gctcgtcctc gcgatcaacc acggcgggcc
    15481 gatgcccctg ctcgcgtgtc agggaacctt caatgacgtt ccctgcggga gcgcgccatg
    15541 aaggcgatgg tctcgctcgg cgacaacgtg atcgagttcg acacctcgga gccacccgcc
    15601 gaggtcagca tcctcggcgt gagctaccgg agggtccgcg ccggcgaggc cgagcgcgtc
    15661 gtgatgctct cagaggttcg ccttctcaag atggagaagc ggatcaacga cctcgagact
    15721 cgcgtccgcc actgtgagga agcgcgcggc ctcgtccggg ggccgcgatg atgcggatct
    15781 gtcagggacc ccggtgcgga cgccagttcc cgcccaaccc gggcgaacgc gggacgaagc
    15841 ggcgctactg ctcgcgggct tgctacgtcc gggcgaaact ctggcgacag cagggccgca
    15901 cctggaaggt cgccgccggc gacgtgacgc gcgagatcct cgacagcctc ggccacgacg
    15961 accgcgagcc gggggagggt ccggactaca ccttccccga cttcaccacg ttcgatgagg
    16021 cgatggcgtg agcgcggccg agccagagca ggtcggcctg ttcgacggcg tcgaggatac
    16081 gcgcctcgag cgcgagttcc gccgcttcca tttcgagaac cccgaggtct accgcgaact
    16141 cgtcgccctg gcccgacgcg cgaagcagca cagccgaaac cgctacggga tcggcgccct
    16201 gttcgaggtg ctccggtgga accggatgct ctcgacgacg ggcgacgact tcaagctcaa
    16261 caacaaccac cgcgcctact acgcgcggga aatcatgcgg cgtgagcccg acctctcgga
    16321 cttcttcgag acgcggcacg tccacggaga ggaacaggcg tgaggccacc gatcacgccc
    16381 tgtcggacct gccgcttctg cggtggatgg cgggacaaca acgccgcgat gtgtcgcgaa
    16441 tgccgcacga agaaggggct ctggtcgaag cacatgaagg gccgcaccgg ggcggaacat
    16501 ccggccttca aggggaatcg accgatcgcg gaccgtaatg gatacctgcg cgtctacgac
    16561 ccgacccacc cgtggcgcag gaacaagggg atcattcagg aacacgttcg catcatggaa
    16621 ctccacatcg ggcggcgtat cgcgcctgat gaggtggtcc accaccgcga tcacaaccgc
    16681 cagaacaaca acctcgagaa cctccagctt ttgaagcgcg gcgagcattc ctcgctccat
    16741 cggcgcgagg actcgcacac cagaaagcgg gacacgaatg gacggttcgc ctaaatgcga
    16801 tacctctcaa ccttctcagg cgtcggcgga atggatgcag gactcgcccg cgccggccac
    16861 accgtcatcg ccctctgcga gcgcgacgag tggagacgct ccatcctcgc cgcccgattc
    16921 cccggcgtcc ccatctttga cgacgttcgt ggagtcacaa aaggcgcact cgagcgacga
    16981 ctccgagagg tgggaggaga gcgaggtggc gccgacgttc tcgccgaggg acgggacggc
    17041 ggcgaccgta ttgacgctca cctcacggaa attccagccc ccgaagaagc tgacaccgcg
    17101 tcaggtcgag gaggccgtgg agttctacac ggcgggccta tcgcttggga agatagcaga
    17161 ccggatgggc gtctctcgcc aatcgatgca cgatcttctc aagcggcgaa tacctctcag
    17221 ggaccggatc gaggcgctcc cacggaagga cgcgacggcg atccggttga agcgggcggc
    17281 cacgctgcgg cgctacagga gccgagcgcg acgaatcaag gaatcggaga tcgcggccgt
    17341 gatggcgcgg gatcgggtgt gtcggatctg cggggacaag ggccgggacg tggatcatat
    17401 ccagccggtc gccctgggcg gacagacgga aatggcgaac cttcaactcc tgtgcttccc
    17461 ctgtcacaag gagaagtcac ggacggatcg tcgcctcgcg gcggcgttga ggaaggaggt
    17521 gcgccaaaag gaagtctcgg acgacagctc gatctgctcg tcggaggctt cccttgactt
    17581 tgccaggacc tctccgtcgc cggaaaacgc gccggattca agggcgagcg atcctccctc
    17641 tttttcgagt tcgcacgaat cgcaggagag cttgttcgcc ccggaggata cgttctggtc
    17701 gagaacgtcc cgggtctttt cagttcccac ggcggacggg acttcgctac cgtcctcggg
    17761 acgctggccg acctcgggtt tcatgatctc gcctggcgag ttctcgactc ccaattcttc
    17821 ggagtacccc aacgtcggcg gcgcgtgttc atccttgcgc gacgtgctcg aggccgacgt
    17881 tgcgccgaaa tacttcttga gccagagagc cgcggcgggg atcctccgac gcgccgccaa
    17941 acgcgggaga aggcgttgcc gggacacttg gaagccgcac tgagggcggt gggatgcgtt
    18001 ccaccgacct cgacgggcac ggagcctacg tcgtgacggg ccccgaggtc gcgcgctcgc
    18061 tctcgtcgca gcgcgacggc tacaacgacg gcagcgacca gacgtacatc gccgcaccgc
    18121 tctcgcacgg ctccaacccg aacagcaacg cggccgggcg caggcgcgag gacgatgtca
    18181 acctcgcggt cgtcccggcc ctcacgtcga agatggcgaa gggaaccgga ggaccggcgg
    18241 gagacgagtg tcagaacctc gtgtggcagg gcggatcaca acaggatcag atcatcgaag
    18301 aaggcggggt cgttccgaca ctcgcccatt ctgcgaacac gcatcaaggg aatcatcagc
    18361 cgaaggttat gaattcccac ggcgtccgcc gcctgacgcc gacggaatgc gagcgtctac
    18421 agggactgac ggacatcgaa aacgcggcta taatagaggt gtgcttgtgc gcccctcgaa
    18481 acgccgatgc cgatgcggcc gcccaatgtc gcagatcgcg gaccaatgcc tccgatgccg
    18541 acggcgtcgg ctcaccgctc gatgcgtcga gtgtggggct gagttccgct tcctgccgtc
    18601 gcgaccccgc gagtgttgct ccgctgcgtg tgcgtatcgt ctccggggaa agagaagcgg
    18661 agatcgccag cgtgtcggca ccgtcctccg gtgcgaagga tgcgggcgag aacttctcgc
    18721 gccgccgagt cgtgcgcgac gacggttttg ctctgtcgct tgctcggctg acgcgaaacg
    18781 tggccctctc aatccatcct ggcgaggagg catcacctcg gagcgggcca tgttcgactc
    18841 ctccgtcgaa tggcgcgccg cgcgtaaggc tgtgtggaag cgagatcacg cgacttgccg
    18901 ccgatgccga cgccgcttcg atcattccct cgacacctac cacgcccacc acgtccgccc
    18961 cttcgcggat cgagatcacc gagtcagact tggaaacctc atccttgtct gccctccgtg
    19021 ccatcgctgg attcattccc gcggaaacgt ggcgcgagag tttctcgatc tcgattaagt
    19081 cgcgcgtcgg ctggacattt cccggggccg actcgcggcg ctacgcgggg ctcggcgatg
    19141 ccgtcacggc aaacgtcgca gcctggatcg gagagcgcct cgcgcggctc ggaggggagg
    19201 cgtgatcgaa cccacgcagg aaggcttctg gggccacctc gagccgccgt accgaacgat
    19261 cgttgccgat ccgccgtggc gttacgccag cgcggcgacg aaggccgacg cgcggaagca
    19321 ctactcgacg atggggctcg cggatatctg cgcgatgccg gtcgccgcgc tcgcggacga
    19381 gtcggcgcac ctctggctgt ggggcaccaa cgcgctcatg tacgaggcgc acctcgtctg
    19441 ccgcgcctgg ggcttccgac cgctcacggt cgtcacttgg tgcaaggccg gcccgggcgt
    19501 cggccactac ctgcgaaaca acaccgagca cgtcatcctc gcgagcaagg ggaagcccgc
    19561 cacgccgaag gagaagccgc tctcgacgtg gttccaatgg gaggcggggc agcactcggc
    19621 gaagcccggt gccttcttcg acctcgtcga gcgcgtcagc gtcggcccct tcgttgagat
    19681 cttcgcgcgg cagccccgcc tcggttggga ccactgggga aaaggcttcg agtcgcgtat
    19741 cgccaacgtc gaaccgaggc tcgccgaggt ggcgatggag gcttcgtcat gagcatttcc
    19801 aagatcgagt ggacggacag gacatggaac cccgtgcgcg gctgctcccg cgtgtcggag
    19861 ggctgccgga attgctacgc cgagcggttc gcggcgcggt actccttcga tgggcaggcg
    19921 ttccacggct tcgcccgctt cggtgcgtcc aagcgcgagg gcggcacgat caccaatcgc
    19981 cccgagtgga ccggccgcgt cgagctgatc cccgagaagc tgtccgagcc gctgcgctgg
    20041 aagaagccgg cgcgcgtgtt cgtcaattcg atgtcggacc tattccacga gcggctcagc
    20101 atcgacgccg tcgccgccgt gttcgggatc atggcctgcg cgccgatgca tacgttccag
    20161 gtgctgacaa agcggccggc gcggatgcgc gcgttcttcg accgcatcca gtccgaaccg
    20221 cgtttcgcga tgagcgtcgg tctcagcgtg gcccccgcga actatcccca cgcgctcaac
    20281 ttcccggccg tctggccgct cccgaacgtc tggctcggcg tcagcgtcga ggatcaggcg
    20341 acggccgacg agcggatccc gctgctcctg caaacgcccg cagccgtgcg cttcgtgtcc
    20401 tacgagccgg cactcgggcc ggtcgatttc gatcgcctcg tcatccccgg cggcacgatg
    20461 gcgccgctct acggcaaggg cctcgactgg gtcatcgtcg gcggcgagag cgggcccggc
    20521 gcgcggccgt tcgcgatcga gtgggcgcgg tcgaccgtca gggcgtgtcg tgccgccgac
    20581 gtcccggtgt tcgtgaagca actcggagcc gaggtcagga tcgacggccc ctccgaccac
    20641 gttcatttcg cgggcgcgtt cgacccggag aaacagttct tcccgcgcct ccgtgaccgc
    20701 aagggtggcg accccgccga gtggccctcg gaccttcgcg ttcgggagtt cccgcgttga
    20761 gcccgcgctc tcccgagttg tacccggaga acgccggcgc gaccgacggc ccctcgatca
    20821 tgggccggat cgacttcgtt ccgccgacga cctcgcacca cgcgaagaag atcgtccggt
    20881 tccaggtcgg tggccgccag atcacgaaac tcggggacac ggacgagctc aaggccgcgc
    20941 gcgcgatctg gtggctcgtc ctgctcccgt tcaagcccac ggaaccgctc gagggcccgc
    21001 tcgcggtcac cctcgacctg acctggccgt ggcgcaagag cgactccaat cggacgcaac
    21061 tgctcggccg catcccgtcc gaggtcaagc ccgacgccga caacgccgcg aagggcatcc
    21121 tcgacacaat ggcgcggctt ctgttcttca agggcgacca gcaggtggca gacctgcata
    21181 tcaggaagtg gatcgggatg cgtcccggtc tcgagttttc aattcggaag attcaattcg
    21241 ggggtgagcg tggagcggtt cctgcgtccg ggcaagaagt acgcccttga gatcgtcgac
    21301 ttcaatcggg tcatcgttcg ttcccaggcg ctgagagagg cgaaagcccg agggcgcctc
    21361 cccttcgtca cgatccccac gaagaacggg gccggcctca tggaggttct ctcacacgac
    21421 cacggcctcg cgctctacgg ggcgttcgtg ctgctcgccc gtgccgcggc cgacatggag
    21481 gtccggggcc tgttcgcgcg ccacgggaga ccactcacaa cccccgagat cgcccgtctg
    21541 gcgatgatcc cgaccgagga ggccgacgcc catgtcgcac gactcatcaa ggtcggatgg
    21601 ctccgattct cggagttgaa ctcggagcag aactcggagg tcgcctctga ggtgaactct
    21661 gaggtcagct ctgatggaag ggaagggaag cgaagcgata acgaagggag agagagtacg
    21721 cgcgcggagc gccgccctcg ggactggcag gaggtgaccg aattctggac cgccaacggc
    21781 ttcctcgggg atccggtggc gttttgggag tggaacgacg accgggggtg gaagtggaaa
    21841 tcctggaagc gcgccgctgc ggcatggagc cgacgggagg gccgtagggc aacaccgcgc
    21901 tcgagcgggc cgaaggacta cgaggccggc gccgctgagt ccaacgccga ggttgccgct
    21961 cgaggggtga tccggtgagc gcggccgagt tcgacccgct gttcgagatc cctgaggccg
    22021 atccggtaac cttgcacgag cccccgaccc aggttactcc tccccacggc cggttcttcg
    22081 cggacctcgc gatcccgccg cgcatccgcg agttcctgac cccgaaaccc tgggaggccg
    22141 gtatcctgat ggggccagcc gggacgggca agaccgccga ggcttgcgcc cgcctcgtgg
    22201 gcgcctccgt ggccgccgac cgcgccgtcg gcctgttcgt ctccatcccc gagctgctcc
    22261 accgcctccg cgcggccgtg ggcgaccccg ccgcccagga ctacgagctc gacctcgtcc
    22321 tggccgcgaa acacctcgtc ctcgacgacc tcggggccga gaaggtcacc gactggaccc
    22381 gcgaccggat ctacctgatc gtgaattacc gcgagtcccg ccgcctcccg accatcgtga
    22441 ccaccaacct caacgtcgcc ggcatccggg cgttcgatga gcgcatcgcc tcgaggctgc
    22501 tcgccctggc cccggtccag accctcaccg gatccgaccg gcggacctgg cgctccgagc
    22561 cgctcgagca gaacgcgccc ttcctcaccg acaagccagc gccgcccaag cccgccgagc
    22621 cctacgtcgc cccgccaacc ccggaggaga tcaagcgggt cgagaaggcc ttcggccgca
    22681 tctacgccaa catgggcctc aagggcggcg ccgcggagtt cgccgcgctc gtccagaagg
    22741 ccgaaccaga acagcggctc atgccccgcg gcgacttcaa cggccgcgtt cgcgaactcg
    22801 ttcgccgcgc caccgccggc gagaagttcg acatccgggc ggaattggag gccaccgatg
    22861 ccgcgtagaa cccacagcca gagcgcagcg gcaggcctac ggggtgcgac gggcgactac
    22921 tcgggcgcgg gcagggtaga accggagatc gcggctctga gggcaacgtc gcgcaattca
    22981 atccccagga cgggtcgcat ctgcccctca tgccgcttct cgaggatgcg cgcccatcgc
    23041 gtcacgaccg acttcgacgg cgagtccttc gtgatccgcc gccgcctgac ctgccgcaac
    23101 tgcggcgccc gcgccatgag ccgcgaaaaa attacgctgt tcgtgatttc cgagaatgag
    23161 cgcgcattct acgcgggaaa agaccccgaa tcgttccgcc ccacggactt tgacagcccg
    23221 gaaaagtagg cgcaccttcg cgccagatgc gcgaagaact cgccgccgca cagatcagcg
    23281 cccggaggct ccggcttgac cgttcgcggt aggccgttcc cgaagggcgc ctcgcccaac
    23341 ccgggcggga agccgaagca gaagccgatc accgaggcat accggcgcct cgtagagtcc
    23401 agcgccgagg cgcttgagaa gctcgacaag aagaatctca ccggggcgga tgttgtcgcg
    23461 ctcgcgatct ttcgccaggc cgcgaagggc aacgcgctcg cggcccggga aatgaccgac
    23521 cggctcgagg gcaaggtcgc gcagccggtc gacgcgaacg tgaacctcgg cgaggccttc
    23581 gccggaatcg tccaggcgct cgccgcgaag aagggaaggg cctcatgagt tccaacgccc
    23641 ccacgcctcc gccgcctccg gtcctgccgg atgacggcgt cgcgctcgcg ccgcacctcg
    23701 tccagcggat ttccttcctg tcgaatcgga tgaggaagga tccccaggag atcgtcaacg
    23761 cggtcctctc gatcggcttc cacgcgatcg accggcgcac gatggacgac gtggtcgcgg
    23821 agttctcggc ggagttcacc gcggcaaagt ccgcgaagat gcccgacccg gagaagaagg
    23881 cgcccgaccc cgacgaccag gtcgaggaga cggccgagca gcggtttgag cggatgcgcc
    23941 gcaatcgcgc actcgcgcgc ggcgaggatc cggacgcgcc gccggcctgc aacctcggga
    24001 tccagggctg cgtcacggtc cacggcgatc aggccgagaa ggagccggga ctcgacgagc
    24061 ggcacctcga cctgctcgcg cagaaggccg cggggaagtg acggttctcg cgctgctcgc
    24121 cgcgccaacg ctcgcggcct ggatcttcgt cggcctgctc gtcttggctg gcctggcgat
    24181 ctggctcgtc tgccggggag cctcgaatcc cgctcccgtg gaggtcaagg cctgtcgcga
    24241 cctccactgc gtctgtcagg tgttgaaggc gctcgcgcgc atggaaaaac gactcgaaaa
    24301 agtggaggaa caactcatgg gagtcaacgc gaacatccag gaactcagcg accggatcgt
    24361 cgagctcaag gcggcccagg ccgacagcgc gcagaaggtc accgacgctt tcgcgcgcct
    24421 cgaggccgcg atcgccgccg gcggccagct ctccgaggag gataaggccg tcgtcgccgc
    24481 ggcgaaggat tcggtcgtcg ccatgaccgg cgccgagcag gcgcagtcgg cccaggccga
    24541 cgccgaggcc ccgccggccg cgcccgaggg atagctcacc gaagggaggg ccactcccgt
    24601 cactgccccc accgaggaac actcctctcc tcgatggttg gcgggggtgg cccgcccgct
    24661 tttctcaaga tgaagcgcaa gcccaagcca acccccgaac ccgcgccgcc ggtcgcgagc
    24721 ctcgccgagg aactcctcgc gatccgcgtc cgcctccaca acctcgagga tcgcgtctat
    24781 tacccgggcg ggtgcgagct ccacccgaac gtccccggcg cgcggtgcga gccgacaccg
    24841 gagccggact gatgaacccc gtcgcctgga cgatcggctt gacctatgtc ccgggctgcg
    24901 gcgggtgggg gatcaggttc gtccacgacg cgcccgaact gccgagcgag gtggtgtggg
    24961 ggatccggtt cggccccttc gttctgctca agacgaggcc gcggtgatga ccccggacga
    25021 gcggctcgcc agaatcgccg aaatagaggc tcggcccgcc ttccaagcgt gggtcgcgga
    25081 ggaagtcgaa ctatggggtt cggccccgtc gccgcgcgag cgacaggcgt gggcgaacgg
    25141 gttcgcggcg gggatcgtag ctgtcgaggt cgtcgatgcc gccgaggaat gttaccgtcc
    25201 tcggggacgt gtggatcctc ggcggacaca ggctgcggtg cggggactcg acgaacgcga
    25261 gcgacgtctc cgcggcgctc ggcgacgggc accccacgct catggtcacg gacccgccct
    25321 acggcgtcga ctacgacccg aactggcgca acgaagccgc gaaggccgga aagctcgcct
    25381 acgcggatcg ccgcgtaggc gaggtcgcca acgacgaccg cgaggactgg acgcccgcct
    25441 ggaagctgtt ccctggcgcc gtcggctacg tttggcacgc cggccggcac gccagcgcgg
    25501 tccaggcgag cctgctcgcg gccgacttcg agatccggaa tcaggtgata tgggcgaaat
    25561 cgaacttccc gatctcgcgc gggcattacc actggcgcca cgagccctgc tggtacgccg
    25621 tccgaaaggg caagacggcg aactggatcg gagaccggaa gcaaacgacg ctgtgggaga
    25681 tcaacctcga caagaacgtc gaaggtgggc actcgacgca gaagccggtc gagtgcatgg
    25741 cgaggccgat ccgcaaccac gacgcgcccg aggtctacga cccgttcctc gggagcggca
    25801 cgacgatctc cgccgccgag caactcggac gccggtgctt cggcctggag ctcgagccgc
    25861 ggtactgcga cgtgatcgtc gagaggtggc agacgctcac cggcggaaag gcgaagcgtg
    25921 agcgcgcagc ctgacctccg gccactcgcg gacttcatcg aggccaccta cgacgacccc
    25981 gcgctgttca tcacctacgg cctcaagacc cacctccgca cctggcaggc gaaggtctgc
    26041 gagcagtacc gccagcagct cgtcgcgggc aagaccgcga tcaaggggac cctccgcacc
    26101 tgccacggct cgggcaagac gttcctcgta gcggggctcc tgctccactg gatgacgacg
    26161 cggtacaact cccgcggcct gacgctcgcg cccgtgttct cgcagatcga gtcggccctc
    26221 tggcccgaga tcgagaagct ccaccaccgc tcgatcatgc ggtccttcgg cttcggccgg
    26281 atgctcaaca cccggatcga cttcggcgcg acctggttcg cggagggccg ctcgagcgac
    26341 cgcccaggac acctcgaggg ccagcactcg gacttcgccg ccatccgcct gatcgacgag
    26401 gcgaaggagg tcgatccagc agtgttcgac gccacggacg gcctgctcaa ctcgccgctc
    26461 tcctgcgacc tgtggatctc cacgccctcc ctcgagtcgg gcaagttcta cgagcgggac
    26521 atggggcctg accccgacgt gatccgggcc gtcatcaccg tcgacgacct catcaacgac
    26581 ccggaaatga ccgaggccga gcgccagggg ttcgcgcggt ggaaggccga gcgcctcgag
    26641 gagtgggggc cgaactcggc cgagtaccag agccgcgtca tggcccagta tgcggagaac
    26701 gccgagggag ccctgttccc ctcgtcctgg gtcgagcggg ccgtgaaggc cgactggtcg
    26761 gtggatgagc gcgatgagtt cggccaaccg caccccgtca tgggcggcat ggatgtcgcg
    26821 ggatcggtgg acggagaccg gacggcggtc gcgatcgtcc gcggcccgga cgcgaagcgc
    26881 cgccatgagt tgctcccctc gatgaagtgg tggcatgagc aggacaccac gaaatcccgc
    26941 accagggcga ccgagatcgc gaaggtccac ggcgcgttcc tgcggatcga ctcgatcggc
    27001 ctcggcaagg gcgtcctgga cggcgcccgc gagctcgggt tcccggccca ggaataccgc
    27061 gcctcggaca agccgatgga tcaggagcgg ttcaccaacc ggaaggccga ggacgcctgg
    27121 cgcgtccgga cgctgctcga gcagggcctc ctcaagctcc cgaacatcca ggaactcaag
    27181 gccgacctcc ggggcatgaa gtacgagatc aagccgaacg ggaagatcca cgtcgtggac
    27241 ccgccgaagt cgcctgacct ggccgatgcg ctgatcatcg cgctcgggcg ctcgagggtc
    27301 ggtatcacct cggcctcgcc ggagtggctt tgaggttcga gggcgtcccg gcatgggtcc
    27361 atccggcggc ggtctgcaag ggggagcgat gcccgttcca cgcgccctcg acccatgtca
    27421 tgcgcggttg gccgatgaac ctgcgcgaga cgggcctgat cgagcggctc tgcgatcacg
    27481 gcgtcggcca cccggatccg gattccgccg cgtggatgga tcggcggttc ggccacaggc
    27541 cggggacctg gtcgagtcat gggtgcgacg gctgctgcgc ggagtccgcc gacctcctcg
    27601 agcggtcgca tgacctccca cggcggtcgg ggccgcgcca cgcccaggac ggggaggggc
    27661 tgtgagggtc gtggagtcca cgctgcggat ccggctcgag ttgacccccg agtcgccgtt
    27721 cttcctcgag gcggaatccg tgatccgggc gaggctcctc acggccttca acgagggggc
    27781 gcgggtgtgg gcccttcgca ccgacgacca actcgtgaac gcccgcccgg aatacgcgcc
    27841 cgaccgccac ctctggccgt tccagaacga gaggcgcgcg tgagccgtcg ccgaaccgtc
    27901 gccgagccgg tcctctgcgc ctgcgggtgc ggggagttcg tgcgtcgcgg ccggacctac
    27961 ctcgtcggcc accatgacac gccgcgggcg cggcaactcg agctcggccc caactcccgg
    28021 cggcgggact acacgggcga gcgcgaggtt cggatgatcg actgttttga cagctacgcc
    28081 ggcgaggcga tggccgacgg cttcgccctc ctcggccagg acggagacga nnnnnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agagccggga ccagatgatc gcgcggctcc
    28261 aagccgagct cgttcgcctg cgcgagggcg gggcatgaac gccctcgcct tctacgccgg
    28321 cgccatgacc atcctcgcgg tcgctggcta tctggcctgg tacggctcca ccatgcagaa
    28381 caagaagctc ctggcggcca tcctggccgt caacgccgac cgggaacggc ttgcagccga
    28441 gctcggtgct acaattgcga agcacaacgc cgagaaatcg gcagcgtctc gccaagttgg
    28501 cgaaggggat ccggctccga actagtggcc gaaccaaccc cgggctccga caagccagct
    28561 tcccgcgtcg agatccccgc agaggcggag ctcaacttcg agctcgccgc gcggtacgcg
    28621 aagaagggtc ccaaccccga atataaggct ttcgaggaac tcgtcgacat cgcctcgttc
    28681 tcctcgccct ggctctctgt ccagactgac atctggcagt tcatcgagga cgccgtcgag
    28741 ggcaagggcg ggttcgtcga cgggacctac ctgatcccgt tcaagcggga ggtcaagcgc
    28801 cgcggcggaa agatcgacga caagttctac gagcgggtcg ccgcctccga ctacgacaac
    28861 ttcgcgaaaa ccatctgcga cggtccctgg aactacatca gccagaaccg ggacgcgatc
    28921 agccgcgaca cgaaggacgc ggagctgcag gccttttgga acgacgtcga cggcaacggg
    28981 accgcgatgg tcgacttcct cgagtatccg gtgcgccagg cgtcgatgta cggaacgggc
    29041 tggatcttca tggaccggcc caacatgggt cccgacgcca cgatcgcgga ctcgagcgcc
    29101 ccggagaacc ggccctacat ctacggcgtc cccacgcgca acgtcgtcga ctgggtgttc
    29161 gacgagaact acgagctcca ggcggtcgcg atcctcgagc acgacggcga cgacgacgag
    29221 gcgaaggacg acccgacgaa gtgcaacctc cgggtctgga ccaagacctc gttcatcctg
    29281 ttcgtgaagg gcgactcgga ccactactcg gtcgacctca agcgcagcgg ccccaatacc
    29341 ctcggggaga tcccctgcgt ccagcttttc aacgagtacc ccggccccgg ccgcggcctg
    29401 ggcgggacga tcatgcccgc gatcgcgcgg ttggcgcgct cccactacaa catgaggagc
    29461 gaggcccgcg agattcagcg taagtgcgcc gccttcctcg cctacccgac gaaggacttg
    29521 gacgccggga gcaagctcga gatcggcagc ggctccgctc tgcccttcaa cgccgacgcc
    29581 gggaagcccg agtgggtgac cccggacctg gcctcgctcg agcactaccg cgaggacatg
    29641 aaggcgacga aggagtcggc cttctccatg agccacatga gcgcgatctc gggcttcatg
    29701 gagaccgacc agacctcgag cggcttccat cagcaggcgg tattcgacga cacgaacaag
    29761 cagatcggca agcgcgccgg cggcgtcgag gcggccgagg tgaaactcgc gcggctgttc
    29821 cagcggatgc gcggcatcgg tggcgagcag ccaaccccgg ccgatgagaa gaagatcaag
    29881 gattcgttct cgatcagcta cccgcgcgag ttcggcctgc gcgacatgag cgcggaactc
    29941 aaccggctcg gcaagcgcct cgagttggag atgggcaagg ccgacgccga ggaggcgatc
    30001 ttcgacttct accagagcgc ctatcccagg gccgaccgga agcaactgcg cgcccgcgcg
    30061 caacagtccg cggccgacta ctacgacagc ctcgcccagg ccaacgcggc gaacgtcgta
    30121 cccatgaccc cgagggatcg ggcgaaggcc atcgccacga aggccgtcaa cggcgagacc
    30181 ttctcgagca agccaaaagc gcgtccggat gtcccggccg cgtgaactga gggaaccaat
    30241 gaccgagaac gcagcagtag cggaaccgat cgagtcggac gctacgaccg acacgcccga
    30301 cgatcgggaa ggggctcctg cgaaggggct caccgaggat cagctcaagg ccaagcgtta
    30361 ctccgcggag gccgcctcct ggcggaagaa gctgaggacc gccgaggctc gcatcaagga
    30421 actggaagcg ggactgggat cgggagacga cgcgggcgac gacaacggct cccctacggg
    30481 ccagaagcgc agcccgtcct acatcgactc cgaactcgcg tccctgcgcc gccaggtcaa
    30541 agaggcgaac gccaaagtcg aggacatcag tgggaagtgg acgaaggccg aggagaaggc
    30601 tcgccgcgta gcgcacgaga acctcgccgg ccggatcgtc gctggcctca actccccgaa
    30661 cacggcgatc gagctgctcc tctccaagac gaagctgtcg gaggacgaga aaaccatcgt
    30721 ctacacgacg aaagacaagg agacgggcga gatggtcgag gtcgaggtga ccaaagagtc
    30781 actcaagtcc tcgggcttgc tcgactccat ttttttccct gcggaaggcg cgaacggaaa
    30841 cgggacaggc acgacggccc ccgtcacggg acggggacac aagctcgacc tctccaagat
    30901 cgacgacgcg gcctacttca aggaacacag ggaggagatc ctcgcgcttc gcggaaagcc
    30961 gaagcgggcg tagaggctct aacagtcaat gtcgaacgcc ctcacctcag tcacgaccga
    31021 cctgtcggtg ttcgggaacc tggttctcga gcagttgcag cctcggctcg tgatgctgcg
    31081 ccggatcacc gtgggtgtcc cggtgccggc aacctacggc gacgaaatcc agatcagcga
    31141 catctcggtc tccggcaacg cgggcacccg cgcggtcggc ggcgccgcga ccgcctcgga
    31201 tctggtcgcc ctgacccgca cgatcacgaa aacgaacatc tacaaggccg tcaagctcga
    31261 caacctggag atcttcttct ccaacatcgg cctcatggag cgcgccgcgc ggcagatcgc
    31321 ccgcgccgtg gctcgcgagg tcgacgccgt catcctgcgg ctcgtcgaca ccgacgttcc
    31381 gtacgaggtg ggccagctcg acggctcgag ctgcatggac gaggcgcaga agtacctgtc
    31441 cctcgcctac aagcagctga tgaagaacgg cgcggacgtc gagtcgctgt tctgcgtcct
    31501 cggcgactcg gaggcccaca acttccggca gaactcgaac ctctacaagg tcaacgaggc
    31561 cgggagctcg gatctcctgc gtcgggccat cctcggttcg gtcctcggct tcgagatcgt
    31621 ggagtcgcag caggtcgtca gctcgacgct ctcgaccaac gcggccacca cgggcacggc
    31681 ttcggtctcg gccgcctccc tgggggcgtc ctcgatctcg gccgcctcgc tcggcaccgg
    31741 cacgatcaag aagggcacga ccttcacgat ctcgacggtg aacaacgccc tcggcaactc
    31801 gatcgggttc gtcgtgacgg cggacgcgac catcacggcc aacgccgcca cgctcccgat
    31861 ctacccgtcg ctcccggtcg ccctggcggg caccgagacc ctcaccttcg tcgaccactc
    31921 cgcggcgggg tcgatgaacc tggcgtggga tcgggactcg atcctcggct acgtcgccgc
    31981 cccggcgccg ttccccgagg gcacgggtgt cgcctccacc acggtcaccg acgaacagtc
    32041 cgggctctcc atccggatcg cggccaagtc caacctgctc gggtcggctg gcaacgccta
    32101 caacaccgag ctcggggccg acgtgatgtt cggcgcgaag gtcatccgcc ccgaaggcac
    32161 cgtgaagatc acgggagagg tctaggccaa tgcgcgtcta cgacatcacg cagaagaacg
    32221 tctccctgac ggccgcggtc cagaccgtga tcgagttcac ggccgcgacc aaccagaccg
    32281 tcgtcgtgct ccgggcctgg atcagccagt ccggctccac gacctcggcg cagcagaacg
    32341 tcaacctcat ccgcaagacg gccgccggca cgaacgccac ggctccgaac gcgaatccgg
    32401 tggacgcctc ggacacggcc ttcgccggca cggttcgcgg cctctgcacc acggagggca
    32461 cgctcggcgt ggacctcatc gcggattcct tcaactgggt caacgggtgg atctggctcc
    32521 cggttcccga ggagcggatc tacgtcaagg gcggcgccat cctgggcctg cgcctaccga
    32581 cggccccgcc ggcgctcacc tgcaacgccg gaatcacggt cggggagctc ggctagtccc
    32641 gatgctcctg tctctgtcct ctcggtcccg cctcgggtca ctcggcccgg ggcgggccct
    32701 ttttctcgcg gagggttcgt gaagcgtcgt cgcggtcgct gtgccggttg gcccgagtgg
    32761 cggcgttgta ccaaccccct gccgagggtc ggatcctggt gtgctcgatg caacttccgg
    32821 cgggtcaagt tcctgccgcc gacccagtcg gaggctcgag ccaaccgcga gcgcggcgag
    32881 cgcggagcga acaggcgggc cgcatgaggg gcatgaggtt cacctccaag ccccgtctcg
    32941 cggtcgagga gcgcggcctg cgcctcgacc cgaacgactt ccgctccgcg cgggagcggg
    33001 cgaacctgac catcccccaa ctcgccgaga agctcggcct cgaggagcac gtcatcagcg
    33061 gcatcgagaa gggctaccga gtcctctcgc cgctccaggc gaaggtcctc tatctccgga
    33121 tcgaactgct cgagcaggcc cgcctcgagg cccaggcgaa ggccgccgcg accgcgcccc
    33181 cggccatgcg ggtcaaactg ccgcagatga ccgagcggga ggaggtcgcc aatggctgac
    33241 cccctgatat gggtcaggaa gccgggacag cccccggtga cctgctacca gtccgcgatc
    33301 cgcttcgccc tctcgaatgg atacgaggag tgctcgccgc ctccggcccc tcaagtggtg
    33361 ccgacggctc ccactggggc cgagggcgct gccaaaaagg gcgagcaact ttccccgccg
    33421 gtcgcggcca agccgagcgc ctacgcgcgc tcgaggcgcc caggtagggc gaagaagttc
    33481 accgaggaac tgcgccgcct gctcaagttc taccgcgaac accccggatc caccgtcgcc
    33541 caggcggtcg aagccctcgg gaccgacccc acctggagcg agctcaacac ggccgacctg
    33601 ttcgccaatg gatacctgga actcgtggag gtggccgctt gagcgacctg accgacgaac
    33661 agatcctcac gatccgagag tcctacctgc gcgaccggta cgtccgcgcg ccggtcacca
    33721 tcgcgatctc caattacaac cgctcggggc gcgagttctt cacctcggac cagatcaaag
    33781 cgatcgacga tgagatcgcg gtgacctggc gggaagtcct cgacgacgtg aacgccgagc
    33841 aggtggccgt ccggatcaac gagggcgaca gccaggacac cgcggacgac tccggggatg
    33901 cgatggagct catcgcgcgg tacaagctga tcgaggcggc gggcatcgag aagatgctcg
    33961 aggaccccgg attccgggcc tccctgatcc ccggcgatga cgcgaagggc gccgaggcga
    34021 tgaagtcctg ggaggcgcgg atcttccgga accgtcagtg ggtcatgggg cgggccggcc
    34081 gcgcggcgat cgaggtcgag cgcaagtgag caacatcacc ccgagccgag gcctggcgag
    34141 ccatgccgag acgatggtca agaccggccg atggaagtcc gaccggcaga aggccgcaga
    34201 gcagcggcgc cacctgggcg tcccggcccg ctgtcaggac tgccgcgacg ggatccacgc
    34261 ccaccggggg ccgatggggt gtctcgaggt cgtcgggtcg ccggacacct acccgccgcg
    34321 ggacttcgtc tgtcagtgta tgaagcagga ggaggtcgcc tgatgggctt cgatccaaac
    34381 caaccgcgcg acgaaaaggg tgagtggacg gccggcggcg gtggctccgg caagatggag
    34441 acccacggcg gcggcggcca cgtgcacgag tcgaaaatga agggcgactg gaagaagggc
    34501 aacgactcgg gcgagcagac tgttctcaag tcgttctcct cgcggtcgaa ggcgatggag
    34561 gtcgcgaagg attccggcct tgaccccgct ggcgttcgtc gcgggaagtt cgggttcgag
    34621 catcccggtc tccgcgctcc ggtggcatcg caggcccagg tcgagcacat gaagcagagc
    34681 aactcccgcg gcctttcttc ggtggccccc ttcaagaagt agatggccga cccgaaggaa
    34741 gccctccgct atgtcgagtc ctccgaatac cggaaggagt acgcgcgcca actggcgaag
    34801 cggctcgccg cggacatgga cattcggcgc gtgatcgacg aaggcggcga ctacgccttc
    34861 tccctgctcg acgacctcgt cgggaagatc aaggagagcc tcctggtcga tgcggtcgcg
    34921 gacgcccgcg cccacgcgaa ggccctcggc cttgagggcc tctcggactt cgagatccgc
    34981 caggtcgtcg cggcctcggt cgagaacgtc ggcaccgagg ccatgcacgg cctcgaggcc
    35041 cgcctcgccg agctcgaccg cgccctgacc aggatggagg acggcggggc ctccctggcg
    35101 accatccggg cctccgtgac ggccgagggc gccgcgaagg gcctcctctc gggcctgtcc
    35161 tcggtcctga cctcgaccgc cgccgggatc gtggccgaca tcgagtccaa cgtcctgcgc
    35221 tcggccgcct tcggcaccga ccagaagatg cgcgaggatg accccgatgc cgagcccccg
    35281 ctctggcggt gggagacccg ggaggacgac aaggtctgcg aggacctcta cgagaactcc
    35341 tgcgccccgc gccacggcga cgaactcacg atggaggagt ggggcgcctt cggggagccg
    35401 cagagcccga acctgatctg ctcgatctac gcgaaggggg cgttctcgaa ctgccgctgc
    35461 gtcctggagc cagcgaacgc cgcggccaac cccgaaccct tgaacatcac cgaggccgcc
    35521 gctcgaggcc gcgagcgcgc tcaagcggcc ttccccgagg tcgcctgatg gccgacgttc
    35581 tcagccagag tccctcgggg gttccactcc ggtcgcccct ccaggtgacc gtcgagtggt
    35641 cgggggaggc cggcaaggcc ctcctctcgg ccgcgcggcg cctcgggcgc gtggagccgc
    35701 tcttgccggt catcgggcgc aacctcgtga actccatgcg gaagaacatc ctcgagaacc
    35761 gcaccccgga gggcaaggcc tggcccgtct tgaagcgcga ccgcggcccc ggcaggaacc
    35821 ccgaccggcg cgccctgttc gactcgggga agatgtacga cgcgattacc taccggctgc
    35881 tcgacgccga gacggtctcc gcgggcatct acgacgtgcc ctacgcgaag ttccagaacc
    35941 agggcgtaca gaagtcgtgg accatccggc cccggcgctt cgggagcaag ctgcggtttt
    36001 ggggcggcga cgggtgggtc taccgcaagg aggtcaccca tccggggatc ccggcccgaa
    36061 ccttcgtcgg gatgcgcgag ggtgacgcgc ccgacttcaa actgatcctg gcgaagcacg
    36121 tcgaggacgc cttcgccacg gccgcgcagg agttcaacgt cgcatgagcg cgatcgccga
    36181 catcgaggcc gacctcgtcg cccgcctgct cgaccagatc ggcgccctct cgccggcgcg
    36241 cgggggaacc tgccgctcgg tgggcgacgt tcggacggcc accatgctcg acaccccgcc
    36301 gccggccgtc ctggtccagt acgcgggcga gcgcgcggag atccccgcgg tgatcggccc
    36361 cacgcgctac cgccagatga gccggttcca gtgggatctc aaggtgatcg ccgcctcctt
    36421 tacgctcgac ggcgagggcc gcgaggacca gcaggagcgc cacggcgcct acaccctgct
    36481 cgactccatc ttcaacgcgc tcgagggcta ccggcccccg aactcgcccc acgcgacccg
    36541 cttcttcttc caggcgaaca gcccctacgg gctgaccgac gcgagcgtga tctacacctc
    36601 gtcctggtac tgcgacgtgt tgaggttcaa gcagtgagcg ccgccgcgcg taggggcgtg
    36661 acggctttct ctcgcgaggg ctgtgccttc gcccgcgagc ggcagagccg cgccaagtcg
    36721 aaaccgtaac tgaaaccgac aacccggatc cggttcgtcc gcgcctgtag ggggacgcga
    36781 gcgcgagccg tgttcgcgag gtaaccaaca gtgtccgacg cacagggctc ttttgagcag
    36841 tggctcgtcg gggtggagac gaccttcaag actccccccg ggacgccgtc tggctccaaa
    36901 gtccccgtca tcaacccgca gatcaagccg agcgtcaatc ggttctactc gaaagcgttc
    36961 accggctcgt ccgaaccccg tccgcctgtc gatggaaaga tcggcgtcga gggtccggtc
    37021 gaggtcgagt gttcgcgcga gtcgctcggc ctgttcctga agggcgtgtt cgggggcacc
    37081 atcgtcgaca gcggaacctc cgctggctac gagcacattc tcaccctcgg ggtccagggc
    37141 tcgcttttcg tcgagcaggg ccacaccgac atcagcgtct acgttctgtg gcgcgggatg
    37201 aaggtctcct cctggacggt caactgtgac gcggagggtc tggctcagat gaccttccag
    37261 ctcatgggcg ccatccagga cgacgccacg acctcctcgt acttcaccgg caccatcacc
    37321 gaccggacgg gctcgagccc gctctcctac ctcgacgccg tcgtgaaacg cgacggggtg
    37381 gtcgtgggcg gcgcgcgcaa gatctcgttc acgctcgatc ggaagttgca gaagaacatc
    37441 gcgatcgacg gctcgtccga atgccgcagc atcacggcga gcgacacccc gaccctcaac
    37501 gggacggccg agttctactt cgaggatgtc aacatcctca acgagacgct cgacggttcc
    37561 aacgagagct cgatggagat cttcttcccc gcggaggagg cgggctgcgg cctcgagctc
    37621 cacatcacga ccctcaagct caagcccacc ggccccgtct cgcagggcca gggcatcgtc
    37681 acgctcccgg cggaattcga cggctacgcc cgcggcacgg cctcggacat ccccgcccgc
    37741 gtgtcgacga agggtttcga gacggtcgcg gtggtcaacg gctccaacga ctccctcgtg
    37801 atctcggtca gcggcggcgc cgaccagacg ttcgacctcg ggacgacggg caacgtctcc
    37861 cgcagcgcga tcgtcaccat cgtcaacgcc acggccacgg gcttcctcgc cgtcgagaaa
    37921 aacgactaca tcgttttcga gacgaccgcg aagggcgccg cggtatccat ccaggtcaag
    37981 gcgtcctcga cgtgcgggac gctgctcggc ctcgacaact cgctccacaa cggacaatcg
    38041 gcgaagtcga tctacgccgt ttaccggaac ctggacgcga ccatctcctg attcaaccgc
    38101 ggggggtggg cgacccgccc cccgctctct ttcgcatcat ggggacgatc cccgagaagg
    38161 tttgaaaccg tgaagttctt ccgcgcctcc gagggcaagg gccgctggta caccccgttc
    38221 cccgacgact tccccgactt caagatcgag atcctccggc tctccaacga gcagatcgtg
    38281 acgctcgccg acaagtggcg cacgagcaag ccggggacga gctcgtccga gaaatacaac
    38341 ttctggcgcg agacctgcga gcagagcgtc ctcaactggg agggggccga gaccctgttc
    38401 aaggccctgt tccccgatgt cacggcgttc gattgctcga aggcctcgaa gaaggtcctc
    38461 atgcgctcgg gcgtgatcga gtgcgacaag gtcaaccgga ccacgggcga ggcctacgtc
    38521 gagcgcgagt cgctctggca gcacctcgac cactggacgg cggatcagga ggcggtcgag
    38581 ctaaaaaact agacgaggtc gcgcgctggc actacggggg ggagtcggac aagcgcggca
    38641 acctcacgag cggcgcggcc tactgcgagc agtgccgagc gaaggttccg aggatcgcgg
    38701 cgaagaagtt tccggaggga acgtgctacc tcaacaaggg caagaaaacc gagaaggagc
    38761 aggtcgagtg cgctcggccc tgcccggagc tcctgcccga gtccgagccc tactggcaac
    38821 tctggcggga cgcgctcgag ggcatggcct accgggggat ggacggggat cccgtcggca
    38881 ttcccccgac cgaggtcgag gccgccgcgc gcctcaactc cctcccgtgg cccgggcctg
    38941 gcgtcgtccg gttccggatc atcaaggcgc ggtggttcgc ctacatggtc gagcaactca
    39001 agaagcgcaa cgccgaggcg gccgcgcggg caaaggcccc gccggtgccc gagccgcgcg
    39061 cacggcgaag gcggtagaat aatggcgcgg caaccccgca gcgttcggct cgaggccggc
    39121 ttggcggtct aggggtcatc ggcaacgaga tcgaacagtc ggtagtcatc cgagtccgct
    39181 cggacggcac cgtccaggtc gccaagctcg gccaggaact cgagcggctc gccggacagg
    39241 gcggcaaggg agcccagggc ctcacctccc tctcacaggc cgcccagggc ctccgtggct
    39301 ccttcggggc cgcccaggga ggcgtcaccg gcatcctcgg agccctcgac ggcctcgcgg
    39361 gcaagtcggg cctcctgctc ggcctggggg ctggcgccgt ggccgccggg gtgggcttca
    39421 tggccctctc gaagggcatc catgaggccg cggaggccga gtccaccatc cacggcctcg
    39481 aggccgcgat ccggcgcacg ggggccgccg cggaggaagt cggccccagg atcgagaagg
    39541 ccatccagac cgtgttcaag acctcggcat tcgacgacga gccgctgcgc cgcgccgccc
    39601 agttgttcgt cacgatcacc ggcaacgccg aggccgcggc caacgcgatc ggccccatcg
    39661 cggacctctc ggccgccctg gggatggacc tggagtcctc cgcgcggctc gtcgctcgag
    39721 cgaccgaggg ccaggtcacc ggcctcgccc gcgcggggat catcttcacc gacaccgaga
    39781 agaagatgct ctcgaccgcc gacgccggga cgcgcctggc gatcgtgatg gggaagatca
    39841 acgagaaggt cggcggcgcg gccgcggccg agctcgacac ctacacgggg caggtccagg
    39901 aactctcgag ggcgtgggcc gaggtcgcgc agaccttcgg ggaaatcttc ttgccggccg
    39961 cgaagtgggt cgtctcccaa ctgaaagaaa tcgcggacgg gatcaagtcg gtcatcgacg
    40021 cgggtcggaa caacttcgcc aacgccccgg ccaacggggt cgccggcggg atcacgaaca
    40081 tcgagcagat cgcggcgaag ggcggccgcg ccggcgggcg cagcatcatc tccgacgagg
    40141 acatggcccg cctggacgag gccgcgaagc gcatgacggc cgtccaggac gccgccggga
    40201 agatcggcct cgggcaggcg gccctcgagg cgatcggact cgcgaggggc ctggatcagg
    40261 tcaacgacca gttgaaccgg ctctcgcagc agcaggccct cgagaagatg gccccggaga
    40321 tcgaggccgc gcgccagaag gtggcagcgt tcgcgaagga cgcccgggac ttcgatgtca
    40381 aggtccaact cgccctccga ccgccgccgg acaacgaagt cgaggctgcg gtgcgcgagg
    40441 tcgaggacaa gttcgacggt ctcatcaacg gggcgaagga caagatcgaa gccctgaaaa
    40501 atgacatcgc cacggcgaag gcgcagcaga cctccctcga ggtcgagtcc cgcctcgcca
    40561 agatggccga ccagatcgac gtcgcgaagg tcacctccct caacgagcag atcgaactcc
    40621 aggacaggat catcgccggg gcgactcgcg cgctcgagaa ggagcagggg atcgagcggt
    40681 cgctcgagcg ccaggccccg gccgcgcgtc agatcgccct ggatgacgcc ctggcgaagg
    40741 ccgccgcgaa ggcgctcgcc gtctaccaga aggtcgagga gtcggtccag aagatcggcc
    40801 aggagggcaa ggcggccctc cagggactcg tcggcgccgg gggcttcggc aagaccatcg
    40861 accaggccgt ctcggacgcc gaggggcaac tgaaactctc cttcggcttc ggccccgagt
    40921 tccgcgccgg cgtccaggaa gccgtccaga agatgcagaa gggcatcgtc gacggcgccg
    40981 tagccggggc gaaggagttc gacgcgaaga tccgcgccgc cttcgacgtg tcgaccgccc
    41041 gcttccagtc ggccgtcgag aacatcggca acgtcctggg cgacatcttc accgacctct
    41101 tttacacggg cgggaagaac ttcggcgaga tcgccggcaa gatgttccag gacgcgatca
    41161 agggcgggag ccaactgctc gcgaaggcga tcggcggcgc ggcgggcaac gccctgggcg
    41221 gcagcatcag ccgcgaccag tacgcgagcg agctcgagta ccagaccgcg gtccagaacc
    41281 gcaccgcgga caccttgaac gcggccgtct ccctgacggg gttgatctcg caactggcct
    41341 cggctggctc gggcgtgaac aacccgcgcc agaaccccgt cggcaccgcg gtcgggacga
    41401 ccctcgcggg cgcgtccgcg ggcgcgtcga tcgccgggat cgtaggcgcc tcgaccgccg
    41461 ccggcggcat catcggcgcg atcgtcggcc tcatcatcgg cggcctgatg ctcgccctcg
    41521 ccccgaaggt gggcgactcc tactcctacg cgacgatggg ggtcaacaag ggcaaagcct
    41581 tcttcgaccc gacgcagaac atcagcgaca acgagacgaa ggcctcgctc gcgaaggtcc
    41641 aggggacgtt cgactacttc tggtcgggct acgtcgacct gctctccaag ttcccgaaga
    41701 tgaagctcga ggggatcctc aaagacatgg ggacgatcgg gccgaccctc ggcctcgaca
    41761 acccgagcgg cgacatcggt cacgccgcga acaaggactt ctggactcaa ttcaacaact
    41821 ggatcgaaca ggggatgccg cgcgacatcg cggccaagtt cgtcggcccg ctccaggagg
    41881 gattcgtctc gctcggcctg tcggcaggga agtttcagca gatatggtcc gcgctcgaca
    41941 acatcgaccc gaaggccgcg ctcgaggcct tgaaccagtt ggccgacgcg ctgaccaccc
    42001 tcggcaagac gaaagactac ttcgcgctct ctggcggcga cctgctcgcg cggggccggt
    42061 cagacgccgc gaagtccttc accgaccagc tcggggagtc cgacgccgag atcctgcgcc
    42121 tcggggaatc cctcaacgag ctcaccggca ccgagcagat cgcgcgcctt cagcagatcg
    42181 cccaactgca agaccaacgg atgcagaagg agatggagtt catccgttcg gtcaacgcct
    42241 cgatcgactc ggtgaacaac tcgatcgact cccggcagcg cgagctcgac ctggccctga
    42301 tgaaggggcc ggacgggaag gcgaacaccc aggcgcagat cgactatctg aagtcctacg
    42361 cggacagcct gcttcagaag atcggctcgg cgaagtcccc ggacgaggtg cgggcgctcg
    42421 agtcggagtc catgagcgtc ctcgccagga tcacggcgct cggcggcaac accgacgaat
    42481 acatgaagtg ggagaaggat caactcgaga cgctgcggaa ggtctcggtc gactccctga
    42541 ccaagatggc cgacgaggtc gccgagcaga acgcgaagtt caccgacgcg atcgacggct
    42601 ttatcaaggc cttccaggac gcgaccctga ccctgaccga cctgacgggc aacgggcaga
    42661 acccgaacaa cggtggcaac atcccgaccc acaccccgaa ctactccggg ggcgatgagc
    42721 ccaccgggaa caacgctcat gggccgtggg ttttcagcga cccgaacacc ccgacccacg
    42781 ccggggactt cttcgacgac tacggcatga agccctggga gcgcggctac gtcccgccct
    42841 cgcagcgcaa cggcgacacc ggagagacca acgccccgat cgcggccccg gcagtgatgg
    42901 aggccttctc ggagttcacc acgtcctcga ggctccaact cgaggccacc caggcgcaga
    42961 ccgcggcgtt gaaccgcatg gcgacggcga tcgagaaggg ccagcacatc gacctcggga
    43021 cgctgaccat caacacgaac ggcgggtcgc ggatggcgcc ggtcgtcgac acgttcgcgg
    43081 cccgccagca gatcgcccgc gcgggagccc gagggaacta ctgatggcga gcatccagcg
    43141 cgacatcggc accctcaccg cggaccagat ccaggccgac ccgtacaagc actacgactt
    43201 cgtctccgtt gacggcgggg tggacttcgg ggtggtgcgg tacaccaacc gcgagcgcaa
    43261 ctggacgggc gacctcgacg gctcgagcca gacctggacc ggcggcgaca actcgggcct
    43321 ccaggtcgag cgcgtctccc aatccaacac ctcgatcctc gatgtctcga gcgtctcctt
    43381 cctcaacgta ttcgacaccg atgtcgccgg tcatgcggtg tggggccagc gcgagatcca
    43441 gttcggcctc aaggatcgcg aggtcgtggt ctacgatgcc tggtgggatc ccgacgacga
    43501 caccgacgcg gggttcatcg acaagttcat cctgttccgc ggcaagatcg gggcgaccga
    43561 gcaccggctt cgctcgaggc tctcgctcgt cccgttccgc gccccgggca acatcccgac
    43621 cggccggatg atccaggaca aggtctgctc cttcgcgctc gcgcgcctct acgggaacac
    43681 gggcgaggcc gcgatgacct gcaacgtgga ccccggcaac accaacggga cgctcgcgac
    43741 cttcccgacc tgccctggta cgccggccgc ctgcatcgag cgcgacaact acgcccgctt
    43801 cggagggttc ccgagaatgc tcaagcccaa cacgacgatc aactggggca acgtgaaggt
    43861 tccgctgtaa tgcccgccac gagcacggtc ctctttcccg agggctacgc cgcggtctcc
    43921 ttctccgagg agccgctccc cggccagctt gcggtcggcg acggcccgga caccggcgcg
    43981 gaacaggtcg ccgagagacg ctcgcggccg ctcatgcggt tctcctggcg ctcggagccg
    44041 ctcgacgtgg ccggggacgc ggcctatcag gccttcgtcg accagtgcgg cagcggcgcg
    44101 acgaacttca cgttctacag gccgtattcc aagttctaca cctcggtcct ggtcggctac
    44161 acggacggga ccgacgcgct cgtggttcct ttccgcaacc tcaactcgtg gacgagcatc
    44221 aaggtcgacg gctcatccga cggcggcgcg agcgtctcca acgagttcgt ctcgccgacg
    44281 aaggaggaca aggtcatcac cctgaccggc ccgccggcgg cggacctgcc gatcaccctc
    44341 gagaacgtct acggccgcca gcggttcatc tgccggatgc tctcctggcg ccggtcgctg
    44401 ttccaggacg gcttcaccga ggccctcgcg ttcgacccgc tctcgacgac agtgccgctg
    44461 atgtacgccg tctacgagtt ctccttccga gagaagaatg gctgatcgcg gccccgtcat
    44521 tcgcgtcgag gacaacaacg agacgcgcac cgtcgacgac cggaggctcc actacgacga
    44581 gcccccgccg tggcaggacc cgacgcagaa caacccgacc gtcgtagacg ataactccat
    44641 cgtgaagcgg ccgatccgcc gcgacatgaa gggtcgcacc ctcgtcttga acgccaacgg
    44701 gcagatcgtc ccgatcctct acgggaagga ccaggccggg gcgaaggtgc cctggggcga
    44761 ggctcacggc ccctacctct acatgatgtt cgtcgtcggc gaggggccgc tcgaggcgat
    44821 cgaggacatc ggcttcgggg acgcgatcga gacctccttc gcgcgcctcg gcctcgtcgc
    44881 cggcaccgac tacaacgtcc acctcgggga tccggcccag gccgtggact cgatcttctc
    44941 cgcgatcgac tcgggctgga tatgggtgtt cccgcggctc gcctacgtca cggtcaagct
    45001 caacatcaac accccgaaca tggatcagat cgacgtgggg cgcttccggt gcaacgtgaa
    45061 gggccgccag gtcgtggact cgcggatcga cccgacgctc gcggacgcct cgagggccta
    45121 ctctgacaac cccgcgctgt gcgagcttga cttcctgacc tcgcggctgt tcggcatggg
    45181 cgcgaagctc tctgaggtca acatcgactc gtccaacgag gccgcggatg actgtgacgt
    45241 ggtcacggcc tcggggctga cggcgcccac cgggacgccc accgacacgg tcaactcggg
    45301 gaccggcacc atcgaggcgg gcatctaccg ccacgcctac accttcgtcg acacgaacaa
    45361 ggtcgagtcc ctgctctcgc cgccctcgac cgaggtcacg gtcatcagcg gcctctcgca
    45421 gcacatcacc gtctcgggcc tcgacgcccc cggcggcggc gtgtttcgga acctctaccg
    45481 ccagaagaag aacccggacg gcacgacctg ggggccgtgg aagcggtgcg agatcctcga
    45541 ggacgactcc tcgaccacgt tcaacgactt cctcccgacg cgcctgctcg gaggttcgcc
    45601 gccggacgaa acggacatcc ggcagttccg gctctcgatg gtcgtggatc aggcggggga
    45661 gacctgcgcg cagaccatcg acaccatgcg ccaatgcttc gcctcccaac tggccttcaa
    45721 caacgggaag ttccaggcct tcgtcgacaa ggttcgcgac ccgacgacca tcgtgttcgg
    45781 ggaccacaac atcgtcgacc tgccgacgat cgagggccgc gacccgtcgc agatccccgc
    45841 ccaggtcgtg gtcaacttca cggacgcggc caacagcttc gcgccgcgcc aggccttcta
    45901 tcccgccgcc ccggtcattg gcgagggcga ctcgaggagc gaccggaaca tcctctcctt
    45961 cgacctgccc tggatcccga ccttcgatca ggccatgcgg atcgcgatct ggatctggaa
    46021 gcggttgaac cgctcgatct cgctgtcctg gcgcaccctc caggtcggcg ccctcccgat
    46081 ccccggctcc atcgtgacgc tcacccacgc gcgggcgggc atccccgaga cccaggtcat
    46141 cgtgacctcc tgcgcgccca cggccgaccg ggtctactgg gacctgacgg gcgagattta
    46201 cgacccggac gactatgacc tcgactacca ctccgaggac gacggggcgc cgccgaccca
    46261 cgagccaccg gatcccccgg acccgccgac gaacctcgtc ctgaccaatg tgccgcgaac
    46321 gggcggcaac ggactcccga ccgtggacgt gaagatcgaa tgggacagcg ccgagatgcc
    46381 ctacgagacc tcgacgctcg tgaggttctc gaccgacggc ttctcgacct tcacggagct
    46441 cggcccgttc gtgacggggc cggtgttcct ctacaacccg accctcggga tccagtacga
    46501 cttcaagctc tacaccgtcc gggtctccaa cgggcaactc tccgcggact cgctcgacgg
    46561 tagcatcgtc ccgtcgttca cggacacgag catccctgag gtcacgcaga tccagatcgg
    46621 gccgaacaac gggctcacct tctacgggcc gacgcccgaa tatttcatca ccacctcgaa
    46681 tgagtccatg ccgccgatcc cggcgccgac gctcgccgcg gccgctggcg gcctcgtcga
    46741 tgacggggat catctggtcg gctcgacgcg cgtcttttcc tggggcgaga cgaaactcgg
    46801 ggcgaccgcg ctcgtgacgc tcggcggcgg caacaaccga atcaacatca cgaacctcga
    46861 cccgtcgccg ccggcgggga cgctgttcaa gaaggtctac gtcacgaagg tcgggcctgg
    46921 cgcggacttc ttccacgcct ccgatgacgc gccggccggg acgagcgact cgctcattca
    46981 cgccgactcc tcttacgtca cgaacgacct actccccgag gagtgccacg gcctcgtcgg
    47041 gtgggaaatc ttcgacgctt ttggcgtggg cgaccccacg accgatccga tgccgctgtt
    47101 caagttcctg ccatggaaca aggggccgca gcggtacgcg gcgctcgacc tgtctcccat
    47161 cgtccacgag gaccccaccg atcacgcgcc gcgcatcgac gtctacatcc gggtcgtggt
    47221 cgttgaggag ggcggcgggg ccgcgaactc gaccggcgtc ctgttctcca agcatcccgc
    47281 gacctggccc ccgactggcc cgccgtcctc gatcccggtc cacgtcacgg tcgtcgatgg
    47341 aatcaatgag gacctcgaca ttccgatcga cgcgaacttc gtctacctgg acgacggcgg
    47401 cctcacggcg gacttcatcc tgggcggcct caccgtcgcg gggtcgacgc cgaccacgcg
    47461 aaagcggatc gtcctggtga acaacacgcc cttccgggcc aacctcggcc acgagagcgc
    47521 gggctcgacc gcggccaacc gcttcgccgt cccgggcgcg aacttctacg acttcggccc
    47581 cgtcctcggc tcgacgacca tcgagtacgg ggtagacgag tccgaccgct ggacgcccat
    47641 cgcgatcaac aacgcggggc aggggccggt cacggacacg gacggcggcg gcggcacctt
    47701 catccggaac agcctcacgg agcagcccga gtcggacatc tcgatctcgg gactcgccca
    47761 cgcgcggcag ttcgccggca acgtggattt cagcggctcc gtggtcgagg gcggcaccta
    47821 cgacgacctc gtcctggacg tgtgggccga cgtggtccac ctcccggcct gcaccatcac
    47881 cgggatcatc ccgcccgacc agggcctcgc cgtggacggc gtcacgccga acaagttcgg
    47941 ggtcatcgtg acgctcgtga acgcatcggc caccggggtc ggcaacggga tcgagttcaa
    48001 gcacaacgac gcgggcagcg cggccgcgaa ccggctcgcg ggcatcgacg acctcgacat
    48061 ggcgaccggc gccgtgttcc gccaggcctt caccatccaa tacgactaca cgaactcctt
    48121 ctggatcgtc gttcggagga acttctgatg aaacggctct gccttctccc ggtcctgctt
    48181 ctgatagccc tcggctgcgc tacggcggcg cagaagcggc gcaacgcctg ggcgcaggcc
    48241 cggaacacct acgtcgcggc gaaggccgtc tacgacggcg cctgccgcgt caagccgcac
    48301 acccgagacg actgtgcgga cttctactcg gagctcgccg cgagctacga cgccctcgag
    48361 gccgcggctc cggccgtcca ggccccggcg ccgatccaga tggccgccgt cgagaaggcc
    48421 gagaaggccc tcgaggccag ggtggcgaag tgaccgcgga ggtgaaggtc gccggcccct
    48481 acgacccgtt ccccgcgctg gccgagctga tgaagctcgc caactccccg gccggtcagg
    48541 catggctcga gcgacggctc gaggacgccg ggatctccca ggccgagctc gaggccaaag
    48601 ccaaagaact gcaaccgccg gagcagccgg cataactcaa ccgtccgggg aagatcccca
    48661 catcttcgga ggcttcatgc gtcggattct cggctccttg aaactcgtgg ccgtcgcggc
    48721 cgcgatcgcg ctcctgctcg cccccgcgtt cgccggggcc gcccccctct ccccgatcct
    48781 cgcctccccc acgatccacg cgcagatctt cgggggcgcg gtccccgagg gcgtcctcgc
    48841 ggtggtcgtg aaggcgctcg cgatcggcgt cctgatctgg aaggggatcg acggcctcaa
    48901 ggacctgttc cccgactgga cggccgctca ccccggcgcg ctacgaattt tcaatgcggt
    48961 cgcggctttg ctcgcctcgc tcggtgtctg ttgggcgacc ggtatggccc acacctgggt
    49021 cgaggtcgga tggtgttcca tccagggcgt tctcgccttc atcacggcgg cgggcctcca
    49081 cgagggcaag tcggcctcgg acaaggctcg agcgggccga taggttcaaa cggggcgccc
    49141 gcggccttcc ccgggccgcg ggcgttataa tcgcggggaa cagagtgggc cagaggcccg
    49201 aaggcatcgt ggctccgctt gaaaaaggcg cccctcctcg ccctcgtcgc cctgctcgcc
    49261 gcttctgccg cgtccgccgg cgacttcttc gcctcgccca ccggcctcga cacgaacgac
    49321 gggtccctgg cgcatccctg gacgatcacg aaggccctct gctcggacgc cctcggcaac
    49381 gcgcagcagt gcaagtggac gccccacgcc ggcgacacga tctggctgct cgacggccag
    49441 tacgacggcc tgttcaacgg caacatcccc ggcagcgcga cggcgcagac ttggatccgg
    49501 ggctggtctc gctgtcccgt ctacgggtcg gcgggtcact gcggcgccat catcaacggc
    49561 ggcatccttc aaaacccctc gaccttccgc ttcggccagt cctacctcgg catccgcgac
    49621 gtggagatca cctcagagcc ggttcgccgc cagaccgagg acccgggttg gggcgggaac
    49681 gagtcgtggc ccagggatgt cgagctcgcg gacgggatca actcggacgc gaccctccaa
    49741 ctgaccggaa acaagatcgt caacgtcatc gtccacgaca ctcgccaggc gctctcgctc
    49801 tggtcgcaga tgggcgacaa agaggcctat ggcgcgatcc tgttttacag cggatggaag
    49861 ggcctcacgc gcggccacgg ccacaacatc tatacgcaga acaacctcgg cgaggaatgg
    49921 atcgagaacg tgtacgcggg ctacggcttc caggaaatgg tccaggccta cggctcgagc
    49981 gtggcctccc tcaacaactt tcgcctctgg cgcaacatcc tccactccgc gtcgtcgctc
    50041 ggcgacctcg gcggtcgcaa cctcacgctc ggcgggacgg ccgacttgaa caacgcggag
    50101 ctgatcggaa actcgatcta ttgcgggccg ctgttcgatt gcggaacctc cgacctgtac
    50161 gtcggctaca actccaactg cgtcaacctc aagatcaacg ggaacacggt gtggggatcg
    50221 tggctgttcg gcaaggggac gggctgcacg accccggcgg aaatgaccgg caacttcgtc
    50281 ggcccgaatc cgaccgcggt ttcgcctgcc gtatgggatg cgagcaagta tccgaacaac
    50341 accttcgcga agggcatcaa gcccgcgaat cccatcgtga acgtctaccc aaacaagtac
    50401 gaacagggcc gcgcccacgt cgcaatcttc aatttccctc agcagccgac cgtcgcggtc
    50461 gatctcggcg acgtgttgaa cgtcgggcag gagttcgagg tccgcaacct cgaggacgta
    50521 ttcaactcgc cgatcctcat gggaacgtgg accggtgccc cggtcaactt cccggcgggg
    50581 aagatgtcga cggcgcagcc gaagggcttc ctccacacca tcgacccgca gcccacggtc
    50641 ggcccaggaa cgccgacgcc ggtcccgacc gagcatccgt accccgtccc ggcgccgccg
    50701 ggaaccggcc cggagttcaa cgcctacctc gtgcgcgcga agatccccgg aaccttcgtc
    50761 ccgagtaaga cgcccacccc cgggggatcg acgccgaccc cgtccgcgac ccccgctcca
    50821 tccgtgacgc cgaccccggc gctactctgg aatcaggagc tcccggtcgc cgcggccgtg
    50881 atcgtcgcgc cggtcgccaa caatggcggc gtcctctcga cgagcgtgga cgggcagacc
    50941 gtcatccccg ccgggacggc gtcctggacc ttcacggtcg cgaacgcaac gacggtgagg
    51001 gtgtgggcga acgtctggac gggagacgcg cagagcgact cctggcatac gcggatggac
    51061 ggtggcccat gggcgacgtt cgactcctcg ctcgactctg gctggcccgc gcccccggtg
    51121 tggaactggg tcaaggtcaa cgctcgagcg ccgttcgaga acccgcgcct gttcgcggtc
    51181 gcggcagggc agcacaccct caccttcggc gggcgcgaga tcggctcctt gcttcgcgac
    51241 gtgtacgtca cgaacgaccc gtccttcatg ccgcctccgt tcgttccgcc ggcagccacg
    51301 gctacgccga ccgcgacgat gaccgacacc ccgaccgcca cggccacgag gaccgcgaca
    51361 tggactttca cggctacatc gacagcgacc ccaaccgcaa gcccgtcccc tacgtcctca
    51421 ccgtcggcca cgccgagccc gacgccgact tcgacttcta cccgaacggc gacggcaacg
    51481 gctacgccct cttttacctc gaccccgacg ctcaccgcga cggcgacccc gaccgccacg
    51541 aaggcgccgc cgacggtgac gccaacggcg acaaagacgg cgacgagcgt ccccccgagt
    51601 aagacgccga cccggacgcc gacctggacg aatcaggtcc gcgaccagca catccgcgcc
    51661 ctcgagatcg tggtctacac ggcgaccccg accccatgag cgtccgattc gacacgatcg
    51721 ctgactacct ggataaccag gactcgccgg cgctgcctgc ggcgcttgcc cccgtgtcct
    51781 tctcgatgtg gttctatgtg acatccatgc cggcgggcgt gaatgcgctc gtgttcaact
    51841 tctttgccga caacctggat gccgcctgcg acatctacgt tcgggagtcc gacaagatgg
    51901 tccaggcgaa caacggtgtc ggcggcgggg ccgtcggcca cgacatcgcc acgatcgcgc
    51961 tcggaacgtg gcactgtgct cagatttcaa agtcctcgag ccagttcaac gtctacctcg
    52021 acggcgcaaa attcggcttc acggaaacgg ctcgcacggt caacccgccc cggaacgctc
    52081 acatcggggg atacggcggg ggctcaaggc tcaacgtggg ttatgtgacc tacgcgcgaa
    52141 cctggacggt cgcgcttacg gatgatgagc tcgccgccga acgctgctcg gcggtccccg
    52201 taaggacgtc cggactctac ggggcgtatc cgctggcgac caacgccaat gacgccagcg
    52261 ggaacgccaa tcactggacg agccacgggt cgcccgtcta ctccgcgttc gagccgacgc
    52321 tgccgaacct ggcgccaatc cccggccgcg ttcgccgggc ccagtttgtt tacggctagg
    52381 agaatcgaat gttgaacctc gtcggaacct ccgcgaaact ccgcgtcacg acctcggcgc
    52441 caggcacgat cgcgatccac tgtcaggcgt catggatgga caacctcaac ggcggcatca
    52501 cgccgggggc gacgaacacg gcgtcgatca cgagcgcgac gacgaccgac attgtcgcct
    52561 cgcccgcgac cgcggggacg acgcgcaacg tcaagggcct ctcgatcgcc aacgcccacg
    52621 cgacgaatca ggcggacgtc gatgtgatcc acaccgacgg gaccaacgcg ctcacgatct
    52681 tccaggcgac cctcgcgccg ggagaggtgg tcatctactc ggacctgacg gggtgggtcc
    52741 acttcaactc ggccgggacg ccgttgaact cgcagagcgt agcgggcagc gatgctcagt
    52801 cctacacgct gacgtcacag tcgccgtgga cgaagccgac ctctttcacg ccgaaattcg
    52861 tcgcggtgtg ggggtggggg gctggcggcg gcgggggagc cggggcctcg ctcgcgaccg
    52921 cgaccatcgc gaagggcggc gcgggcggtg ggggcggcgc gtacaacctc aagatcttcc
    52981 tcgcctcgga gctcggcgcg a
//
LOCUS       DAOMFY010000002        45026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_253_length_45026_cov_8.552801, whole genome shotgun sequence.
ACCESSION   DAOMFY010000002 DAOMFY010000000
VERSION     DAOMFY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 45026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45026
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_253_length_45026_cov_8.552801"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLB01_00385"
     CDS             <1..621
                     /locus_tag="PLB01_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQQ75782.1"
                     /translation="AMLQGMTAHYLATSTRPLGPGDTCLVHAAAGGVGLLLCTIAKKR
                     GARVIGTVSTPEKAALARAAGADEVILYTEQDFVAETKRLTGGRGVAAVFDSVGKTTF
                     EKGLDVLAPRGTMALFGQSSGAAPAIDPQILNAKGSLFLTRPSLFAYVASRDELLARA
                     ADVLGWVKDGSLPLRIDRELPLAGAADAHRALEGRGTAGKILLMPG"
     gene            complement(627..1484)
                     /locus_tag="PLB01_00390"
     CDS             complement(627..1484)
                     /locus_tag="PLB01_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQQ75783.1"
                     /translation="MTFPSASLWALAAAALALGAWLLHRYRVSRLVRRERELTRELRE
                     KTLELKEKTAELEQSNALLAETSLTDKLTGLKNPRFVEAAIGTLLAPARKPVRDPEQR
                     TECVAILLADVDGLDDVNDAFGGEGGDAVLQAVADAISASVRGGTVVARWSGDEFLVV
                     ARVRFGGEAGALAVRLRQAVASWVKAGPGEGAKPVTVSIGFSVEPLVRARPHLASFRD
                     HLALAAGALRRAKAAGGNLACGWRVNDEALEAAISLHGEPGALRVLRKPDEAETAGLI
                     SLALEHREG"
     gene            complement(1542..2525)
                     /locus_tag="PLB01_00395"
     CDS             complement(1542..2525)
                     /locus_tag="PLB01_00395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQQ75784.1"
                     /translation="MKYTTLGRTGVTVSRLALGCMSYGTPAWRPWVLTEEQAQPFFRR
                     ALEAGINFFDTADMYSLGASEEVTGRALRTYGRMEEIVLATKVHFPMTDGPNMGGLSR
                     KHVVQGCEASLRRLGVETIDLYQIHRWDGSVPIDETLAALDQLVAQGKVRYIGASSGP
                     AYKLAKALALSDARGWSRFAAMQNHYNLLYREEEREMLPLCREEGVAVIPWSPLARGL
                     LAGTRDSARAAATTARGATDDYTGRLYDSPSDPDVIAAVRKVAAARGCPPAEVALAWL
                     LSRPGVTAPIVGATKLEHLEAALKAVDTVLTDEEIAAVETPYQPHGVRGFR"
     gene            complement(2530..4596)
                     /locus_tag="PLB01_00400"
     CDS             complement(2530..4596)
                     /locus_tag="PLB01_00400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ75785.1"
                     /translation="MSRLRIQVLAAVLTALALAPAARAADSPAAPAPAAAKKAFGVDD
                     LWAVKRVGAPVLSADGARAAYAVTAYEGDEMKANADLWLVDVASGASRRLTANKASDT
                     SPAFSPDGKRLAFLSKRDGDAAAQLYVLPLDGGEADRLTEMPTAVANPRWFPDGTKIA
                     FLANVVAGAESPEETKKALEKREKNPVKAKTSENRLYRYWDHWLTDGEYPHLFVVDVA
                     TKKVTDLTPGSKRYFGLDEGGSYSIAPDGQSLVFAANSTSEPYTTLNWDLFLVPAAGG
                     EVKNLTPAREGEDVNPVFSPDGKTIAFGTQLKADGWPDYTRLALLDVATGRVALLTDG
                     WENSCAGWAFAKDGKSIVFTAEVRARTNLYAVPLAGGVPRVVWKGGTAAAPSVTPGGE
                     VVFQRHDLNHPPEIAAVKLDGTGFRMLTRVNDALADSWNLGAVEDVTFKGAAGDEVQM
                     FVVAPPGADAGKKYPLVQLIHGGPVGTFGDAFSFRWHPHAFAAPGYLVAMVNFHGSSS
                     FGQKWVESILGAHPDKPFTDVMKATDLLIAQGRVDPARMAAAGGSYGGFLVNWIAGHT
                     DRFKCLVSHAGVYDLMAQFASDATYGRHHSYGGSPFTNRDAVEKWSPNRYAAAFKTPV
                     LILHGERDYRVPIGQGLEFYGTLTAKGVPARLVYFPDENHWVLKGPNAKLWYSEVLGW
                     FGRWMK"
     gene            4671..5390
                     /locus_tag="PLB01_00405"
     CDS             4671..5390
                     /locus_tag="PLB01_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HQQ75786.1"
                     /translation="MSSVPPPGSGYPPPPPPPPQGPPPVMPSGPGLAWETQAIGPESF
                     FDTAKLFITAPDQAWARTRETGDYTRPLLFALVIAWIGAIFNAVWSTMFGAGLMKMLP
                     AQYSRFGAMGMGGGGLLANIILAPIFIVIGLFIGSAIFHVSFMIVGALAGSRSQFEGT
                     FRVVSYSSIAHIAYVIPFVGGLVALVWRIYLMMLGVQQLHKANQGKALMGILLPVVLC
                     CGCAAIGMIFAGAALFSAFNR"
     gene            5393..5770
                     /locus_tag="PLB01_00410"
     CDS             5393..5770
                     /locus_tag="PLB01_00410"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HQQ75787.1"
                     /translation="MAPGRQLAFLWGGAALACAAAVPFARDLAAGTAPCAFKAFTGVP
                     CPTCGGTRALLAIARLDPGAALAWNPLVTLGVVVFFAGGFVALVRALRGSGVPDAAPP
                     RWAGAALGLALAANWAFVVVAGR"
     gene            6052..7437
                     /locus_tag="PLB01_00415"
     CDS             6052..7437
                     /locus_tag="PLB01_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75788.1"
                     /translation="MSGGFLPAATVRSYALVGVCGIPAGAKAISLNATAVDPVGPGFL
                     TLWEKGQPVPPVSTLNFKAADRVANAAVVPLSPDGSVSVAFGVSGGDLVLDTNGYYAI
                     APGVTSLNALIGDVTLLPGANVTLTPAGNNLTISATGGPGGQLPPGASGQTLRSNGTA
                     WIPNDALTSDGVNVTVAGKLEMGISPRATAGSLDFLHGDSATGNVGLGFDSLPVGASG
                     ISNTAYGTSSLTALSSGAENTGVGSGALNSNSTGGANSAVGSAALSANTSGNFNTAMG
                     FFSLRSNTTASYNTAIGRGTLFLNTTGASNVAIGDMAMASNTSGPTNVAVGSGALQNN
                     TVGSGNVALGSAAGLNVVSGSNNVFLSHQGVAGDNGVVRIGTAGSQTATFIAGIRGTT
                     TALNDALPVVIDSAGQLGTISPTVMANVPVMVLIELQNMRRRAEAAEAGLRALEQRMA
                     KLEAAGARAER"
     gene            complement(7434..8318)
                     /gene="rfbD"
                     /locus_tag="PLB01_00420"
     CDS             complement(7434..8318)
                     /gene="rfbD"
                     /locus_tag="PLB01_00420"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HQQ75789.1"
                     /translation="MALRIVVVGAGGQLGQALVRELRKTGHEVAALTRAALDVTQRFA
                     VLDRVAKLQPEVIVNATVLGVDASEDDPKLALAVNAFAVRSLADVARSANAALVQYSS
                     DYVFDGTATQPYRETDKPNPVNVYGATKLLGEIYAQNVPRGYVIRVEALFGTPGSKGT
                     IDKMIAEAKQGRTITAFYDRKVSPVHVDDVAAATRRLVEKKATAGIYHCVNSGFATWH
                     EIALEIVRLAASPSNVIPASLERSGLRTARPLFSALSNEKLANAGIDLAPWQSALERA
                     VREAPAPAKPASGVRPRA"
     gene            8371..9348
                     /locus_tag="PLB01_00425"
     CDS             8371..9348
                     /locus_tag="PLB01_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75790.1"
                     /translation="MSACPEVAEAVAALRAAGVLTAEEGERPGRVARGELVSVRAELR
                     AAFYLGVLLLVSGVGLFLKENVDRIGPSAIAGAVGLAAGACLAWAWRKAAPPTWGEAA
                     PSHPAFDYVLLLGALLAAADLAWVEAQTRLLGPAWPLHLLVVALFYGALAYRFDSRML
                     LSLSLASFAAWRGLALSVAHASLGPGDVVRLRWEALATGALFAAVGVATASAEKKAHF
                     GDVWVNAGALLVFGGLLSGVFGSELDWGVWLVALLGVSAAVAAAAYRWKRTLPFAQAV
                     LAAYLGSMRAVFSQGLGGGGFEARFLLMAMLGGAALATIFFAHRRMRIP"
     gene            9345..10529
                     /locus_tag="PLB01_00430"
     CDS             9345..10529
                     /locus_tag="PLB01_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75791.1"
                     /translation="MKREWVAAERVEEVRAAARGWKRAGGIGEGAFEEISRRYPEPRT
                     LPAPLWRILTFVFGSFILLAFAAFFALTVRPSGSSAWVLCAVLGVGFVIAADLQARSP
                     AMALRGGVEAASFWGIVLLIGALFLLFEENLHLSEPDGPNLVLLGAVLLLAVGAFRWG
                     NAVYAGFAAGALFVLLARAPHGRLLWIAGGVALSAVAGRFTDQPSWAPSHRACAWGLV
                     VCGIAAVYAAVNFWSLDHRTIEQLGRHARSLPESWPGDGARAAAILATALVPLAVLLR
                     GVVRRRKILLDTGLVLLALSLVTLRAYVHALDVRVVLAGAGALLIGGALALNRWLSRG
                     SGGERRGFTADPLFGDETAFRAVELAPVLAAHAPAAKPADEPGFSGGGGGFGGGGAGT
                     SY"
     gene            10609..12024
                     /gene="vorB"
                     /locus_tag="PLB01_00435"
     CDS             10609..12024
                     /gene="vorB"
                     /locus_tag="PLB01_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HQQ75792.1"
                     /translation="MASPAPVRERPKPFLLEEFCKGCGRCIEACAKHCIEVGHEINPL
                     TGLVPVVLNLDHCNACGLCIDACPEPYGLRPEAKAKSGEDFELQDPAKLFGDKLSARP
                     KPVAIPPEIVPLPKTEPLVLKGNYAAAVGALLAGCRHVYGYPITPSTEGAELMAKLLP
                     RLDGVFLQAVSEVATVNMMYGCGGAGLPCMTFTSSPGFSLMLEGISYMIGSEVPGVFV
                     DVMRGGPGLGNIAPEQSDVKLACRGLGHGNTHAIVLAASTPQEMLDLSRLAFELSFKY
                     RNPVILLADGYLGQMTGKVVLPEAMVKPGLPEWAVFGDAAHRGNLISSIYLDEKDLET
                     FNVHLNEKYARIEAAEQRADLFLCDDADVLVVSIGTPARMAKGAVEALRRQGVKAGLF
                     RPVSVWPFPIDALKPLLPKARRVVVVEASAGQLEDELRLALSHAGLACPPIESVRHMG
                     GVLPQASEVVKAVQAREEVTA"
     gene            12021..13442
                     /locus_tag="PLB01_00440"
     CDS             12021..13442
                     /locus_tag="PLB01_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQQ75793.1"
                     /translation="MSVFYENFERHSGGQGLKAHATHYCPGCGHGLAHKFLAEAIEEL
                     GVQDRMVAVSPVGCSVFLYYYFDVGNTQAAHGRAPAVAIGHKLANPESIVVSYQGDGD
                     LASIGLAEIVSTAQLGIPITVIFVNNAIYGMTGGQMAPTTLMGQKSSTSPDGRSAFMG
                     QPMKMAELIAGLDGPVYVERVALYDAKQRIKAKKAITKGLRMQVEGRGFAFVEVLAEC
                     PTHLGLTPEETEHWVKESMLPVFPLGVKKDVVVEPHAKAPAPNFDGARVASLVGASAE
                     KPPRFAKGFPEHLGGRDVALKLAGAGGDGAQTAAMLIARAAISEGFDATHIPSYGPES
                     RGGTSYADVHVAEDEVLSPASPHPHILVAFNAPSLAKFAPDVVPGGTIVYDSSVIHDV
                     PKVADGVKLVGVPFTEIAVDLGKAMVKNVVALGALCAATGLFPEDTFRTALKQALKDK
                     CALIPLNEEAFAWGVKSVTTPGH"
     gene            13454..13729
                     /locus_tag="PLB01_00445"
     CDS             13454..13729
                     /locus_tag="PLB01_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75794.1"
                     /translation="MEHNTTHSLTPAEWRLVNALREVPESALRTRVNALVEGLIELAR
                     EPKCPEHMADGVPCASVHANCDQCVQVTRMLEGLEAMQLQMRGYPGA"
     gene            13726..15948
                     /locus_tag="PLB01_00450"
     CDS             13726..15948
                     /locus_tag="PLB01_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQQ75795.1"
                     /translation="MTPRALLEHEVYALLKSAGFEVPRHIFLPGVPEKAERQLEDFLE
                     GLGKEDAAGEVVLKIASPDLAHKSDVGGLSLSKKSSSSVLAAAKKIWDDVALRAPGAE
                     RLGILVVEKLVPASGTPAAEALLSYKNDAAFGPVLVFGLGGLLTEWYGSLAAGHTTAI
                     LRPGEVKQGLQAAVEKSPALKIFFEGNRARAKAPLELDAVALLLETLSKNLLFSFSPS
                     NSLGNPTLEELEVNPILLCADGRWVAADGKARFSSRRAERASRPLGKISSLLSPRSAV
                     VIGASASGPNPGRIILKNLKQSEGVAYGKLWAVHPKEKTIDGVPCVASVADLPQKVDL
                     AVVSVPAPAAPGVVAALADGKAESIILIPGGFGEAGRGDLEAALRKALADGHARPDGG
                     PVLVGGNCLGIVSKRQYNTFFLPQYKLPFHDAPGDSLVAVSQSGAYLVSLTSNLDGIV
                     FPRASISYGNQMDLTAADFLTYFADDPSVRLLVFYVEGFAPGDGERFVEAARRVAAQG
                     RRVVVFKAGQTPLGAEAAKSHTASLAGDYAVAKALLEGAGVQVAQTLDMFEDYTKVHT
                     MLAGRPAAGRRLAVLSNAGFECSAVLDKLYGLVPSALMPATLARLKACLPGIAHAANP
                     VDATPMADTPAFVEAAAAMLDDPGVDMLLVSPIPVTPALDILAPDPFGAHAENLFSKS
                     SLAQELLRLFRATQKPVVVNVDSGRLYDDFVMVLQRGGIPVFRKIDRASRALAALCRA
                     "
     gene            complement(15953..16780)
                     /locus_tag="PLB01_00455"
     CDS             complement(15953..16780)
                     /locus_tag="PLB01_00455"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HQQ75796.1"
                     /translation="MSKPYTLQELLIIAAAKEIHDHENVILGIGIPTTAGALAKALYA
                     PNATLMMESGIVDFQPLLPLNHIADAHACRGFSYGTDLFSTFTMTYRGFVDVCFLGVG
                     QIDKFGNVNTTVIGDYDKPTLRLPGSGGAPDFFSYAKRTVLTMLGGTFVNKLDYFTSP
                     GHLDGGDSREKSGLFPKGSGPSMVLTTKGVFRFDPVTKEIYLAQVHPKVTVEDVKRDV
                     PWDLKVAPDLSVTPRPTDEEIAFIRNFAPNVSMSRELMLELTLANVTRKMKTAGAIR"
     gene            complement(16773..17795)
                     /locus_tag="PLB01_00460"
     CDS             complement(16773..17795)
                     /locus_tag="PLB01_00460"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HQQ75797.1"
                     /translation="MSVKDAISRFVHDGDQLVIGNYTVGSCAELVYEVARQEKKGLAL
                     FSQSGIFDVEILIGAGCVSKLVTAYVMRAGGKKGGSAAERALKAGTLQLEDYTNFDYN
                     ARLVAGMHGMTFMPVLEGVMATDLFRKRGFMGENKFRVIECPYTGKKILTVPAANPDV
                     CIVHVQRADKFGNAQYWGALGSIAAAALASKHIIVSCEEIVDHEIVQSSPALTLIPAY
                     RVNAVVEVPWGAHPTEVLGYYNLDSAMYGVFTQADSTADGLKAWMDEWVYGCENRAAY
                     IDRYVAKFGSGALDSLKAKPYYSAPANYGAAVTSRWDRDGREIFTGLTLPEMETILKE
                     RGQLYE"
     gene            18008..18928
                     /locus_tag="PLB01_00465"
     CDS             18008..18928
                     /locus_tag="PLB01_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ75798.1"
                     /translation="MSIRFDGRVAIVTGAGGGLGRCYALELARRGAKVIVNDLGGALD
                     GTSGSAGLAQAVADEITAAGGIAVANHDSVADRSAAQRMVDEAASRFGSVDILVNNAG
                     ILRDKTFAKLELDDFQKVVDVHLMGSVNMTKAAFPLMKDKGYGRIVFTTSSSGLYGNF
                     GQTNYAAAKLGVVGFMNTLKLEAKKYGIKVNCIAPTAATRMTGGLWPPEVLNAVPPER
                     VVPAVVYLCSEGSPNGHIIEAGGSYFARVAIVEARGVVLGPNATAEDVAARYEEIADL
                     TGARTFDSGMEVATNIAKAAAEAFPAAVTS"
     gene            18989..20686
                     /locus_tag="PLB01_00470"
     CDS             18989..20686
                     /locus_tag="PLB01_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQQ75799.1"
                     /translation="MSDPSRFQNSRPLEKPYAVWRSAAGAVLVIGSLACSTAPPASPQ
                     ASAPAAPTVVANGERLVGEYVGKGGSEAVFRGIPYAAPPVGELRWKAPAPITPRPGVQ
                     QATKFGPSCVQEVEGNYFYRYIAETLGQDPKLVPLPGPSSENCLFLNVWTANLGGKDL
                     KPVMVWIHGGANAVGTAVGLQTDGANLARKGVVVVSFNYRLNLFGFLAHPALTAESGH
                     GSSGNYALLDQIAALKWVQQNAAAFGGDPGRVTVFGESAGGTNITYLLATPLARGLFH
                     RAVIESGGYAVSDFRPLGEAEALGKSFEEALGVAGSKDVLAAMRAVSPEDLRRAWLRT
                     RKLTGNAPNVDGWVFPRATAKIFDRGEQHKVPVLIGFNKDEWTTLRQYWPNPTLDDFR
                     KVIRAVWGPFADQVLKLYPATTDAEAAAAVDRYSPDWYYVGPTRFIAERTTRAGSPAY
                     VYLFSRVPQAPGGEKLGAHHAMEIPYVWDTLNAIPWMPRKPYDHELAGAMSAAFVRFA
                     ITGDPNGGKLPAWPRYKSGSEDFLEFGDTIRASSGIRPEFCDLYEKLLASWATAARP"
     gene            20714..22027
                     /locus_tag="PLB01_00475"
     CDS             20714..22027
                     /locus_tag="PLB01_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HQQ75800.1"
                     /translation="MDANTLSTLGIVVSLVFIITLALRGWHIIVLAPLAAIIAALFSN
                     LNVLETLTGPYMTGFTNYARRFYLIFLMGTLFGKFMEDSGAARSIAQAILRLIGGSAE
                     TPLRVLVAFALVPMALTYGGVSLFVVIFTVLPIARHLWQEADIPWHLFMLSFVFGAAS
                     MTMTMLPGTPQIQNLMPTKYMSSTPASAPVLGLLGAFVVFAINVLYMRYCVRKFKREG
                     EHYVPPAGAAGPAAPERGTPHAGLSLLPMAAVLIFLNVPAVKVDVIYALGIGILVTML
                     LFWRFIPNVVDTITRGAANTVVPIVNTCADVGYGMAIAATAGFRVVSAWLLTLPMHPI
                     VSLSIATNIMAAITGSASGGMGIVLETLAPKYLALGLSPHLVHRIVVMSAGCFDAMPH
                     NGAVITMLAVAGLTHRQAYKHIFVGHVLATLVALIVVIPLGILLY"
     gene            22041..22880
                     /locus_tag="PLB01_00480"
     CDS             22041..22880
                     /locus_tag="PLB01_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ75801.1"
                     /translation="MSRLKDKVCVITGATSGIGKRTAEIFAAEGAKLVIAGRREAEGR
                     AIARALGGACDFVRTDVTLEAEVEALIAFALEKHGRIDCLFNNAGGPGPVGGIEEIPV
                     AGFDAAMAVLVRSVVLGMKHAAPAMLRQKSGSIINNGSVAAHRAGYSSSLVYSAAKAA
                     VVHLTRCVAMQLGEGGVRVNSISPGFIPTGIFGKALGMAAEEAERSAETVKSGLGRVQ
                     PIPRAGLTDDVALAAVFLASDDSTFVNGHDLVVDGGMIGGRGWTQHQETLRGLKRAFG
                     LDG"
     gene            complement(22891..23871)
                     /locus_tag="PLB01_00485"
     CDS             complement(22891..23871)
                     /locus_tag="PLB01_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019470013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQQ75802.1"
                     /translation="MKTLASFLALVLLGAAEVGRAELPTVVAEDWLLVTNAAETPEGL
                     IFDRDGNLYMVLVGNGSVLRSAPPYKTVTTVYGPSKSRFATVKIHKDGRLFLCDLGEK
                     AGYRGDATGRIVAMRPDGTGMEVIVPAGRFTPDDMVFDGKGGFYFTDFKDYTDAANVA
                     NGAVYYVSPDFKTITPVLKNLASPNGVALGRNGKTLWVTETFHQQLHRLDLGADGVTM
                     NLYGHMIPYRFNGFGGPDSAMIDADDNVYVAYFGAGKVIVFNALGNLIGQVLLPGSDK
                     GAMLATTTMAILPGTDELIIGSGDFGGRGARIFKAKAFGKSWGGAYQFQK"
     gene            complement(23891..26227)
                     /locus_tag="PLB01_00490"
     CDS             complement(23891..26227)
                     /locus_tag="PLB01_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQQ75803.1"
                     /translation="MSGRRRWAVLAAVAAGVIASATLAFLFLRGSRPTAHRAAASNVI
                     LITLDTFRSDRLSCYGSRIVDTPNIDAFAAEGVRFTNAASTVPLTLPAHASIMTGLYP
                     PGHGVRENVGTVLDGRIPTLAELLGKSGWTTGGFVSAFVLDHRWGIGRGFDRYFDDFD
                     LGGLGTANVANVGLVRRDGADTVAAALNWLDKRPAGRPFFLWLHLFDPHDPYVPKEPF
                     KSRYPGRPYDAVVAYTDSLVGDFRRALDERHLLKDSLVILTSDHGEGLGDHGETFHGF
                     LVYDTTIHAALIVRPPGGVRGGRVVDRTVSHVDLLPTILDLVGLKAPENVHGRSLAPL
                     VAGRDVPWDRPVYSESLYPLLHYGWAPLRALRAGRFKLIDVPRPELYDVAQDPTEEQN
                     LHSVDPNRARELTADLGRLRHRIESGKLPAGNAPEIDQRAVARLRSLGYLAGGGGVAV
                     GKESERVRADPKDRLQLHQKIVDAQSRISHREYDAAGRLLEQVLAEDAEIVDAHQMLG
                     QIAAERRRPRDAIRHFKRALELNPMHEQSLFDLAAEYADLGRWDEALAGFRRVLELKG
                     ADARASLAIADVYVRLKRFDEAADVLDKASQSGESAPFFANKMGEVRVEQGRDDEAVP
                     QFERAVALDDGFALPHFNLGFLREKRGEGRLAAEQYARALELEPAFFRAQFNLGRLHA
                     AMGDVERARQSWEAFLRSNPGSVQGRYYFAKLLMDTGGDLVRAENLAREGIALDPGHA
                     EGPLGYYVLADLLNRRGRAAEARAAAATGRRIEEAVKR"
     gene            complement(26224..26859)
                     /locus_tag="PLB01_00495"
     CDS             complement(26224..26859)
                     /locus_tag="PLB01_00495"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ75804.1"
                     /translation="MKARQRPETTTATPARPRRRASRRDEILRRAAHLILEQGFEMIS
                     LVDVARACEISKAGLYYHFNSKQELLAAIISYAQDIHEDVIRNQLTDSAADAADDLRR
                     LIHSHAVMMTREDDAAFAILAVDEMRSLLPADRERVARRKRFYLGLIRERLERLAAAG
                     KLRNVDITAATHTLAGMVLWLPKWYRQPGRLSAAQMADEITELAMHAVLPD"
     gene            26978..29782
                     /locus_tag="PLB01_00500"
     CDS             26978..29782
                     /locus_tag="PLB01_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ75805.1"
                     /translation="MSKTAKCFILSVLLLASASGFAQITGSIHGEVIDSEGQPLPGAT
                     VKLTGDPIPGAERIATTDAKGAFRYGALPVGRYAVSAAVPGFKTQQVKDVRVSIDAVA
                     SVRIRMQLQTVSESLTVTATAPLVDVVSSGVAVNYDSQFVQSLPTRNNFTDIIAVAPT
                     VSAPNEGNPLLAAYGGNVTSQQWNIDGLNLASPEGGWLGWLINPEIVQETSLKGFGAG
                     AEYGSTAGNVYNMVTKSGTNAFHGSAGGYWTNNSLTGSNVKLDPSTLYSYRLWDPAGT
                     YTIDDYYDARATLGGPILRDKLWFFGAGQWDKINIVGPNGVAGIDGSGTTDYRYDGKL
                     SWQIADGHHVDVRGHTANQKTVPAPDMYTALSAVVTYDIDINMITGDYTGILAPSTLL
                     NLRGGYWSKNQDMASRTGSNEEWLQEAIYPGPALNKGGIFWFSGRKENYTQADAVLSQ
                     FASDFIKGSHEFKFGVQYNEGAGQRNAAKSTFRWRQPPSPGFADKYWEFRYQILPPLI
                     YGAETTTWGAFLTDSWKISNRLTLDLGVRFDDQKGKIPSYPKLDLAGNPTDVMLPSAD
                     MIHWSNFAPRIGFAWQPTKDGRSVLRGFYGRFWDGPVSSAWYYPPPGRGNSEVWFVYP
                     YQFRVSSVAAPPPDQLLAPGVKNPFSDQFAVSYDHQIGSEFAVGAQFIYKHTDDMIGW
                     QIENDGVCKQFLWDDPWTTNVKEQIPLCELTKNPTLHKGNGPGPGSLAPGDTYHMYYR
                     GAVLTFKKRYSKGWDLMASYTYSKTEGINPRPHYNGSLGQGLPVFSSDEGSDPNDWYN
                     AYHLQEGDRTHMFRVQSNVDLGWGLRASGVFNWQSGRPYQRLANVIGPTTGTVITVTA
                     DASDSLRLPSQAILDLGLQKTFNLGKGVNLDLGLQLLNALNDDAVEYYSSMVLFPGQN
                     FEPSAWVGPRRLVLRAQVTF"
     gene            29837..31030
                     /locus_tag="PLB01_00505"
     CDS             29837..31030
                     /locus_tag="PLB01_00505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQQ75806.1"
                     /translation="MPNKVVIVKAVRTPIGAIGGGLAGFQAQQIATHAIKALIGSAGI
                     DPAAVEYTCMGWVMQDPRCPNVAKTAAELAGVPPTVPGTTYQENCASGGAAIHSLCRR
                     ILLGEVSLGIAGGVESMSNVPRYLFAGRTKNQMYGDMTLVDGLFGALTDAYVGSGELM
                     GLLTERLVAKYGVTREEQDQVAYRSHHAAVDAWEKGAFADYVVPVEVPQKRGAPAVVA
                     RDEGPRPVTHEQLAAAKPYFKRDGGTITSANASTLNDGAAVVLLASEERARELGLEPL
                     AEIVAYHNVGVERELMGEGAFKVIPPLLKRAGVDLSEVDYLEINEAFAAVVAAAYHFL
                     PGLKAERTNQWGSGISLGHPVGATGARQVVDMVHQLRRRGAALGVTSRCVGGGMGSGE
                     VLRRL"
     gene            31106..31375
                     /locus_tag="PLB01_00510"
     CDS             31106..31375
                     /locus_tag="PLB01_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75807.1"
                     /translation="MSQGNQPVVAMLSPAEMELIRLLREPSTSPVRARVKTVVEGLLM
                     AAKEPFCPEKPCPQAPGECDRCVQTMRVLEGLEAMEMQIKGLPGA"
     gene            31409..33547
                     /locus_tag="PLB01_00515"
     CDS             31409..33547
                     /locus_tag="PLB01_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ75808.1"
                     /translation="MRTASLRLVLAAMTIATTNTPAQTASVPPTPPVAKKVEKVHLLH
                     GDPRPDPYFWLREKSNPQVAAYLEAENAYTDAVLKPTEPLQKALYDEMLSHVKETDLS
                     VPYRDGAFLYYYRTEKGKQYRIHCRKPAADGAEQVLLDLNALAEGKKFMSLGAFRPSD
                     DGNLLAYSTDDTGFRQYTLAVKDLKTGALLPERMLRVTSIAWAADGKTLFYSVEDETT
                     KRSHRVYRHRVGTPASADALIYEEKDERFNVGVGRTRSRAWIVMESSSHTTSEARLLK
                     ADAPEGEWTLVAPREQDHEYYVEHRGDLFYIRTNSGGRNFRLVTAPVAAPGRANWKEL
                     VPHRADVMLTRIDLFKDHLVLEERANGLPRFTVRSFATGDSHVVSFPEPVYTASSETN
                     RVWDTKVFRYGYQSFVTPQSVFDYDMDARKATLLKEWEVPGYDRTHYASARLWATAAD
                     GTKIPLSVVWRSKTAEGKARTLKDGPFPTLLYGYGSYGIPIGVSFRSDRLPLLDRGFV
                     YAIAHIRGGGEMGKAWHDAGRMKNKMNTFTDFIAAGEHLVAEKIAAKDRLAAEGGSAG
                     GLLMGAVVNMRPDLFSAVIAHVPFVDVLNTMSDATLPLTVGEYEEWGNPAKKDEYDFI
                     KTYCPYTNLRKGAYPAMLVKTSFNDSQVFYHEPAKYVARLRTLKTDANPLLLKTNMAA
                     GHGGASGRYDFLHEIALDEAFLLTRFGLAK"
     gene            33559..34032
                     /locus_tag="PLB01_00520"
     CDS             33559..34032
                     /locus_tag="PLB01_00520"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQQ75809.1"
                     /translation="MSAEDHANVPVIPPVLFGLGIGAGYLMRWLAPVRLVPLRLEREA
                     VWAGAALSLLGLAFGGWAFLTFVRARTTPHPNHPVSALVTWGPYRVSRNPMYVGLSGF
                     VAGLALVANTPWLLAVLPFVWLALRRLVIDREEAYLTRRFGEEYRAFLGRTRRWL"
     gene            complement(34043..35209)
                     /locus_tag="PLB01_00525"
     CDS             complement(34043..35209)
                     /locus_tag="PLB01_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ75810.1"
                     /translation="MLRFLPFVLKNLLRKKTRTVLTVGSILLPVFLVAFMATFLRTLN
                     LPDPARERGLYRLVTRHKVGLTTSLPYAVVNRIAALEGVRAITPIDRFAGLYRDASAG
                     NVFPRFAVDPQTFLDVFDDAKIVQGSADAWKRDRTGGLVGVALVKKYGWRVGDRVTIT
                     GTLYPVDLTFTIQGVYSLPYENSASIFFHRAYLEEAWPPFRGTVSTLWTRCTDAACAE
                     RLPKEVDAFFENSPNPTKTESENAFTLGFVSLLGNVQLLLTAMGVIIIGVVVVIAANT
                     VALNVRERVVEVAVLRTLGFSRPRIVGLVMSESLALAGAGGVLGFLAFLAAFSRLKGA
                     LMETRMAPFAAGMKIFPEVVALAGLVALAVGVFSAIVPAVMAARRPIVEGLRAS"
     gene            complement(35212..36489)
                     /locus_tag="PLB01_00530"
     CDS             complement(35212..36489)
                     /locus_tag="PLB01_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HQQ75811.1"
                     /translation="MDDRTVFARRRARFFDAMKEGVAILFATPEAPFGHDVHYRYRPD
                     PDLFYLTGFAEPGATAVLDADARTFTLFVLPRDRERETWEGRRAGPEGAVKTFGADQA
                     FPAAERERRLPDLVRKARVLHHALGLSGANDRLVAGLLARFRREARHPKRGPVTISDP
                     TDLLHAMRLLKSPEEIALLERAAAIAASAHRDAQRTARPGRYEYEIEAAVDRRFRAMG
                     ASGPAYPTIVASGENATILHYVENSRRLAAGDLVLVDAGCEFGGYASDVTRTFPASGR
                     FTRPQKALYGAVLAAQRAAIAAVRPGAPWDAPHEAARAVLLDALLHLGLLKGRREALK
                     KKNAAQRFALHHTSHWLGLDVHDRGRYRDERGRPRSLEPGMVLTVEPGLYVRPDETRV
                     PKEYLGLGIRIEDDVLVTESGARILTEDAPKAC"
     gene            36528..37430
                     /locus_tag="PLB01_00535"
     CDS             36528..37430
                     /locus_tag="PLB01_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQQ75812.1"
                     /translation="MDSRIVPALRARYPEFSGARLKRAVLEGQVTVDGAVVNDPGAFV
                     AAGAEVRWDRDRRIERRVATALKVLYEDDDAIAVLKPSGLLTHPTEAKEKDTLLARVS
                     TYVQKRHGGGARTYVSIVHRLDKETSGILVFARSRRGLIGLQAQLRAHTMDRRYRAVV
                     EGDLVGDAGTFDKDLVAEPGERRRGVVKAGEKGLKAITEWTVLERFGIATLVEARLKT
                     GRTHQIRIHFANAGHVLVGDSVYRDRKAKPFPVPFPRQALHAGHLGWKTEEGKAVSLD
                     AEPPGDFLKLLDDLRKRGKKRRSS"
     gene            complement(37576..38208)
                     /locus_tag="PLB01_00540"
     CDS             complement(37576..38208)
                     /locus_tag="PLB01_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQQ75813.1"
                     /translation="MKVPVTLLAAAVGLSLVGCATTTDIDKKIAESQQKTDKKIESVE
                     TQVEQLQEGQKAQGGKIDEQGKQIEQLGQSAKDALARAQEARVLAKGKIVFQQSFAED
                     RVRFKVNSFELTKEAKSALDEFAVKVLALDKGVYLEIQGHTDDTGGTDYNDQLGQQRA
                     EAVRRYLARQHKLPLGRMSTISYGDTLPVESNKTAVGRSKNRRVVIVVLE"
     gene            complement(38229..39083)
                     /locus_tag="PLB01_00545"
     CDS             complement(38229..39083)
                     /locus_tag="PLB01_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ75814.1"
                     /translation="MSVGYVMVPFVLTDLKGKPVADLQEKDVTLLVDGQPVLTDLFSR
                     SDDAPVSFTILLDASGSMGLVGKMEGARAAVRALVAQKLPGDDFSLYAFSSGAVREVV
                     PFTADTAKLLAAMDAVTPHGRTAFYDALARMPDRTLLGRNGSRAILLLTDGIDNASEL
                     TRDDLATLLEGVDVPVYPIGLRSPGARVTPPPGITPEAMLNLDVLGHVARLSGGILSI
                     VDEAAQLPAAIARVEKDLRTQYLVGFTPTGAGPVRYRRISLKLSGPARPVRVRAGYRG
                     TLPPIKGG"
     gene            complement(39338..39751)
                     /gene="ndk"
                     /locus_tag="PLB01_00550"
     CDS             complement(39338..39751)
                     /gene="ndk"
                     /locus_tag="PLB01_00550"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQQ75815.1"
                     /translation="MEKTFAIIKPDAVESKNAGKILSRIEAEGFRVVAFKRHNITKAE
                     AESFYGEHAAKGFFSALVAYMTSGPVYVAVLERAGAIAHWRATMGATDPGKADAGTIR
                     KEFGQSIERNAVHGSANPADAAREVTFFFPAIDLL"
     gene            39822..40274
                     /locus_tag="PLB01_00555"
     CDS             39822..40274
                     /locus_tag="PLB01_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HQQ75816.1"
                     /translation="MKNFFEVSNRRGLLDRLDGLRPDAAHLWGKMDVAQMCAHCAIAL
                     EVAAGDVPKTQAFIGKVLAPFVKKKFLHGTEPLSKNSPTDPTFVVSDSRDFAREKARL
                     VEIAKRFGEAGAAAADGRVHSFFGRLTGEEWGVLMYKHLDHHLRQFGA"
     gene            40336..40581
                     /locus_tag="PLB01_00560"
     CDS             40336..40581
                     /locus_tag="PLB01_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HQQ75817.1"
                     /translation="MATLTLKNVPEELVDQLKEEAKQNRRSLNQEALSRLESTIARRR
                     RSSEEVIADLRRLHKRLGSRPIMTDALIERAKREGRP"
     gene            40578..40985
                     /locus_tag="PLB01_00565"
     CDS             40578..40985
                     /locus_tag="PLB01_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQQ75818.1"
                     /translation="MIVVDTNLIAYAVVPGERTEAALAVLDFDSQWVAPRLWRSELRN
                     ALVTTMRAGKLSLSGALGAFEEADRLVTDTDLEEETEACLRLAEKGGISAYDAEFVVV
                     AERLGMPLVSSDRRLAKAFPGRVLSPEEFVARG"
     gene            complement(41047..41925)
                     /gene="sucD"
                     /locus_tag="PLB01_00570"
     CDS             complement(41047..41925)
                     /gene="sucD"
                     /locus_tag="PLB01_00570"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQQ75819.1"
                     /translation="MGANWISPDTKLVVQGLTGREGRFHALASRDYGTKVVAGVTPGK
                     GGTAVDGIPVFDSVAAAVKATGANASMVFVPPPACADAIMEASDAGVALIVAITEGVP
                     VNDMIKAKEYLARGTSRLIGPNCPGIIRPSAKVKIGIMPARIHKAGFVGVVSRSGTLT
                     YEAVGQTTSLGFGQSTCIGIGGDPINGTNFIDALELFRDDPETQAVIMIGEIGGSAEE
                     EAAAWIKAHLGKPVVSFIAGRTAPPGRRMGHAGAIISGGKGTAGEKVEALKAAGVHVA
                     DSPALMGETLKKALGK"
     gene            complement(41927..43093)
                     /gene="sucC"
                     /locus_tag="PLB01_00575"
     CDS             complement(41927..43093)
                     /gene="sucC"
                     /locus_tag="PLB01_00575"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQQ75820.1"
                     /translation="MKIHEYQAKEVLRGFGVATPRGRVTDSPEEAGRIAKEFGGRAVV
                     KAQIHAGGRGKGGGVKVAKSPEEAEALARKILGMQLVTHQTGPAGQKVKKVLVEEALD
                     IAQELYIGITLDRAIGKPVMMVSKQGGMEIEVVAAKDPSAIVKEPFEPSWGLQPYQAR
                     KLADALGLTGETHKKAVGFLIALAKAYVATDASLAEINPLLITGAGDVLALDAKMNFD
                     DNALFRHTAIREYRDLDEEDPLEVEASKFGLNYIKLDGNVGCMVNGAGLAMATMDIIQ
                     YAGGKPANFLDVGGGANEEQVKNAMRIILSDANVKAVLINIFGGIMRCDIIATGVVNA
                     VKEIGIKVPVVVRLEGTNVEEGKAILKSSGLAVIPADGMKDAAEKVIASLGKAA"
     gene            43150..44547
                     /locus_tag="PLB01_00580"
     CDS             43150..44547
                     /locus_tag="PLB01_00580"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ75821.1"
                     /translation="MRFALLLGLLAAAPSAFAQSSARGGRSEDVSVLPAAAAADVARQ
                     VASELKLGDFAALESRFDERMKAALPDAALRVFWADTLKRAGALRGCAEPRTSFAGEF
                     TLAFAACTFEKQKAELRLTIRPDGRLAGMFLAPESAARREWTAPDYVTPAAFSERETS
                     VAAGLVTLPATLSMPKGEGPFPGVVLVHGSGPHDRDETVGGTRVFQDLAQGLASRGIA
                     VLRYEKRTKAFAKELAGTRDMTLKDEVLDDAVAALGVLRAAPGVDAAKVFLLGHSLGG
                     LLAPRIALLDDRTAGVILLAAPSRPMWEVVRDQIEALSGPRASESQRATAPALRREAD
                     ALADLYAGRPMPPGTATIFGAPPSYWLELKTQTPALTLSGLGLPVLVLQGGRDYQVSM
                     KDFAGWQRALKDVSRAAFRSYPKLNHLFVAGEGPSAPAEYEKTGHVDRDVVEDIASFV
                     KRGTLRPVETKKPKK"
     gene            complement(44552..>45026)
                     /locus_tag="PLB01_00585"
     CDS             complement(44552..>45026)
                     /locus_tag="PLB01_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ75822.1"
                     /translation="FARRGERAARAAAAPGTLVEAAKLDMTKPWAEQGVAPGSFDAVY
                     SVNCFHVAPDLGFVLAEAKAALKPGGVVVVSECVKPGAHPPPSYVDFVFDFLVSFTDV
                     RTDPVLRPNHGFLSPAAWRASFAAAGFRGVEVLPDVDAVAARFPKFFVGALVARS"
BASE COUNT         6808 a        15391 c        15651 g         7176 t
ORIGIN      
        1 gcgatgctgc agggcatgac ggcgcactac ctcgcaacgt cgacgcgccc gctcgggccc
       61 ggcgacacgt gtctcgtcca cgccgcggcc ggcggcgtcg ggctcctcct ctgcacgatc
      121 gcgaagaagc gcggcgcccg ggtcatcggc accgtctcga cgccggagaa ggccgccctc
      181 gcccgggcgg cgggcgccga cgaggtcatc ctctacacgg agcaggattt cgtggccgag
      241 acgaagcggc tgacgggcgg ccggggcgtc gcggccgtct tcgactccgt gggaaagacg
      301 acgttcgaga agggcctcga cgtcctcgct ccgcgcggga cgatggcgct cttcggccag
      361 tcgagcgggg cggccccggc catcgacccg cagatcctca acgcgaaggg ctcgctcttc
      421 ctgacgcggc cgtcgctctt cgcgtacgtc gcctcgcggg acgagctgct cgcgcgggcc
      481 gcggacgtcc tcggatgggt gaaggacggt tcgctcccgc tccggatcga ccgggagctt
      541 ccgctcgcgg gcgccgccga cgcccaccgg gcgctcgagg ggcgcggcac ggccgggaag
      601 atcctcctga tgccgggctg acggcgtcag ccctcgcggt gctcgagcgc gagcgagatg
      661 aggcccgcgg tctcggcctc gtccggcttc cggaggacgc gcaaggctcc cggctcgccg
      721 tggagggaaa tcgccgcttc gagagcctcg tcgttcacgc gccagccgca cgcgaggttg
      781 cccccggcgg ccttcgcgcg ccggagcgcg ccggccgcga gcgcgaggtg gtcgcggaag
      841 gacgcgaggt gcggccgcgc ccgcacgagc ggctcgaccg agaacccgat cgagaccgtc
      901 acgggcttcg cgccttcgcc ggggccggcc ttcacccacg aggcgacggc ctgcctgagg
      961 cgcacggcga gggcgccggc ctcgccgccg aaccgcacgc gcgcgacgac gaggaactcg
     1021 tcgccgctcc agcgggcgac gacggtaccg ccgcgcacgg aggcggagat cgcgtcggcg
     1081 acggcctgga ggaccgcgtc gccgccctcg ccgccgaagg cgtcgttgac gtcgtcgagc
     1141 ccgtcgacgt cggcgagcag gatcgcgacg cactcggtcc gctgttcggg gtcccgcacg
     1201 ggcttgcgcg ccggcgcgag gagcgtcccg atcgccgcct cgacgaagcg cgggttcttg
     1261 aggccggtga gcttgtccgt gagggaggtc tcggcgagga gagcgttcga ctgctcgagc
     1321 tcggccgtct tctccttcag ctccagcgtt ttctccctca gctcgcgcgt gagctcgcgc
     1381 tcgcggcgga cgaggcggga gacgcgatac cgatggagca gccacgcccc cagggcgagg
     1441 gcggccgcgg cgagagccca gagagaggcg gacgggaacg tcatcgcgtg attgccggcc
     1501 gcgttattgg atcacgaacg gacgcggccc gcccgggcgc ctcagcggaa tccgcggacg
     1561 ccgtgcggct gatacggcgt ctcgactgcc gcgatctcct cgtccgtgag gaccgtgtcg
     1621 acggccttga gcgccgcctc gaggtgctcg agcttcgtgg cgcccacgat gggtgccgtc
     1681 acgccgggcc gcgagaggag ccacgcgagc gcaacctctg cgggcggaca cccccgcgcg
     1741 gccgcgacct tccggacggc cgcgatcacg tcggggtcgg acggggagtc gtagagcctc
     1801 cccgtgtagt cgtccgtggc gccgcgggcg gtcgtcgcgg ccgcccgcgc gctgtcgcgc
     1861 gtgccggcga ggaggccgcg cgcgagcggg gaccacggga tcacggcgac accctcctcg
     1921 cggcagagcg gcagcatctc gcgctcctcc tcgcggtaga ggaggttgta gtggttctgc
     1981 atggcggcga agcgggacca tccgcgggcg tccgagagcg cgagcgcctt cgcgagcttg
     2041 tacgcggggc ccgagctcgc tccgatgtag cggaccttgc cctgcgccac gagctggtcg
     2101 agcgccgcga gcgtctcgtc gatcggcacg gagccgtccc agcggtggat ctggtagagg
     2161 tcgatcgtct cgacgccgag gcgcctgagg ctcgcctcgc aaccctgcac gacgtgcttc
     2221 ctcgagaggc cgcccatgtt cgggccgtcc gtcatcggga agtggacctt cgtcgcgagg
     2281 acgatctcct ccatccgccc gtacgtccgg agcgcgcggc ccgtaacctc ctcgctcgcc
     2341 ccgagcgagt acatgtcggc ggtgtcgaag aagttgatcc cggcctcgag cgcgcggcgg
     2401 aagaacggct gggcctgctc ctccgtgagc acccacgggc gccacgcggg cgtcccgtag
     2461 gacatgcacc cgagcgcgag gcgtgagacc gtgacgcccg tacggccgag ggtcgtgtac
     2521 ttcacgggcc tacttcatcc agcggccgaa ccacccgagg acctcggagt accagagctt
     2581 tgcgttcggg cccttgagga cccagtggtt ctcgtccggg aagtagacga ggcgcgccgg
     2641 gacgcccttg gccgtgagcg tgccgtagaa ctcgaggccc tgcccgatcg ggacgcggta
     2701 gtcacgctcg ccgtgcagga tgaggacggg cgtcttgaac gccgccgcgt agcggttcgg
     2761 actccacttc tccacggcgt cgcggttcgt gaagggggag ccgccgtagg agtggtgccg
     2821 cccgtacgtc gcgtccgacg cgaactgcgc catgaggtcg tagacgcccg cgtgcgagac
     2881 gaggcacttg aagcggtccg tgtggcccgc gatccagttc acgaggaagc cgccgtacga
     2941 gccgccggcc gcggccatgc gcgccggatc cacgcggccc tgcgcgatga ggaggtcggt
     3001 cgccttcatg acgtccgtga acggcttgtc cggatgcgcg ccgagaatcg actcgaccca
     3061 cttctgcccg aagctcgacg agccgtggaa gttcaccatc gcgacgagat agcccggcgc
     3121 cgcgaacgcg tgcgggtgcc agcggaacga gaacgcgtcc ccgaacgtcc cgaccggccc
     3181 gccgtggatg agctggacga gcgggtattt cttcccggcg tcggctccgg gcggcgcgac
     3241 gacgaacatc tggacctcgt cgccggcggc gcccttgaac gtgacgtcct cgaccgcgcc
     3301 gaggttccac gagtccgcga gcgcgtcgtt cacgcgcgtg agcatccgga agcccgtccc
     3361 gtcgagcttc accgccgcga tctcgggcgg gtggttcagg tcgtgccgct ggaagacgac
     3421 ctcgccgccc ggcgtgacgg acggcgccgc ggccgttccg cccttccaga cgacgcgcgg
     3481 aacgccgccc gccaggggca ccgcgtacag gttcgtccgc gcgcgaacct ccgcggtgaa
     3541 gacgatcgac ttcccgtcct tcgcgaacgc ccagccggcg caggagttct cccagccgtc
     3601 ggtcaggagc gcgaccctgc ccgtcgcgac gtcgaggagc gcgaggcgcg tgtagtcggg
     3661 ccagccgtcg gccttgagct gcgtgccgaa cgcgatcgtc ttgccgtccg gcgagaagac
     3721 cgggttcacg tcctcgccct cgcgggcggg cgtgaggttc ttcacctcgc cgccggcggc
     3781 gggaacgagg aacaggtccc agttgagcgt cgtgtacggc tcggacgtgc tgttcgcggc
     3841 gaagacgagc gattggccgt cgggcgcgat cgagtagctt ccgccctcgt cgagaccgaa
     3901 gtagcgcttg ctgccgggcg tgaggtccgt gaccttcttc gtcgcgacgt ccacgacgaa
     3961 gaggtgcggg tactcgccgt ccgtgagcca gtggtcccag tagcggtaga gccggttctc
     4021 gctcgtcttc gccttgacgg ggttcttctc gcgcttctcg agcgccttct tcgtctcctc
     4081 cggcgactcc gcgccggcga cgacgttcgc gaggaacgcg atcttcgtgc cgtccgggaa
     4141 ccacctcggg ttcgcgacgg cggtcggcat ctccgtgagg cggtcggctt cgccgccgtc
     4201 gagcggcagg acgtagagct gggccgcggc gtcgccgtcg cgcttcgaga ggaacgcgag
     4261 gcgctttccg tccgggctga aggccggcga cgtgtcggag gccttgtttg cggtgaggcg
     4321 gcgcgaggcg cccgaggcga cgtccacgag ccagaggtcc gcgttcgctt tcatctcgtc
     4381 gccctcgtac gccgtcaccg cgtaggcggc gcgcgcgccg tcggccgaga ggacgggcgc
     4441 cccgaccctc ttgacagccc agagatcgtc gacgccgaac gccttcttcg cggccgctgg
     4501 ggccggcgcg gcgggagagt cggcggcgcg cgcggcaggt gcgagggcga gggcggtcag
     4561 gacggcggcg agaacctgga ttcgaaggcg tgacatgcgt tctcctcgac ttgacgtttc
     4621 ccgagcctag cagcgatagg atcagaccga ccaatcgaga ggaggactcc atgtcgagcg
     4681 ttccgccgcc tggatccgga tatccgcccc cgcccccgcc gccgccgcag ggcccgccgc
     4741 ccgtcatgcc ctcgggtccc ggcctcgcct gggagacgca ggcgatcggg cccgagtcgt
     4801 tcttcgacac ggccaagctc ttcatcacgg cgcccgacca ggcctgggcg cgcacgcgcg
     4861 agacgggcga ctacacgcgc ccgctgctct ttgcgctcgt catcgcgtgg atcggcgcca
     4921 tcttcaacgc cgtctggagc acgatgttcg gagccgggct gatgaagatg ctgccggccc
     4981 agtacagccg cttcggggcg atgggaatgg gcggcggcgg cctcctcgcg aacatcatcc
     5041 tcgcgccgat cttcatcgtg atcgggctct tcatcggcag cgcgatcttc cacgtgtcgt
     5101 tcatgatcgt gggggcgctc gcgggctcga ggtcgcagtt cgaggggacg ttccgcgtcg
     5161 tgtcgtactc gtcgatcgcg cacatcgcct acgtgatccc gttcgtcggc gggctcgtgg
     5221 cgctcgtctg gcggatctac ctgatgatgc tgggcgtcca gcagctccac aaggcgaacc
     5281 agggcaaggc gctgatgggg atcctgctgc cggtcgtcct gtgctgcggc tgcgccgcga
     5341 tcgggatgat cttcgccggg gcggctctct tctccgcctt caaccggtag gcgtggctcc
     5401 cggacgccag ctcgcgtttc tctggggcgg cgcggcgctc gcctgcgccg ccgccgtccc
     5461 gttcgcgcgc gacctcgccg cgggcacggc cccgtgcgcc ttcaaggcgt tcacgggcgt
     5521 tccgtgcccg acgtgcggcg ggacgcgcgc gctgctcgcg atcgcgcgcc tcgaccccgg
     5581 cgcggccctc gcgtggaacc cgctcgtgac gctcggcgtg gtggtcttct tcgccggagg
     5641 cttcgttgcg ctcgtccggg cgctacgcgg gtcgggtgtc ccggacgccg cgccgccgcg
     5701 ctgggcgggc gccgccctcg ggctcgccct cgcggcgaac tgggcgttcg tggtggttgc
     5761 gggaagatag ccttcgttcg accccggcct cgattgatcg gaaaggggtt ctccatgcga
     5821 atcgtccgcg cgtcactcgc cgcactgctg ctcctgagct tcctgtccgt tcgtgcggaa
     5881 gagccgctcc tcggaacgtg gccgtcgccg cccttctggg ctccggctcg tcaggtggag
     5941 tcaaggagcg aatcgagtcc ggcgcgaggg gccctcgcgg ctccggttcc cctcccgttt
     6001 ttctcagtcg ctccgtgtcg gctcgtggac acgcggggta atggggctcc gttgtcgggc
     6061 gggtttctgc cggccgcaac cgtccggagc tacgcgcttg tgggcgtctg tggcatcccg
     6121 gcgggcgcga aggcgatctc tctcaatgcg accgccgtcg atcccgtcgg cccgggcttt
     6181 ctaaccctct gggagaaggg gcagccggtc cctccggtgt cgacgctcaa tttcaaggcg
     6241 gcggacaggg tggcgaacgc cgccgttgtt cccctgagcc ccgacggaag cgtttccgtg
     6301 gcgttcggcg tttccggagg cgacctcgtt ctcgacacga acggctacta cgccatcgcg
     6361 ccgggcgtca cgtcgttgaa tgcgctgatc ggtgacgtca ccctcctgcc gggcgcgaac
     6421 gtcacgctga cgcccgcggg caacaacctg acgatttcgg cgacgggcgg gccgggcggc
     6481 cagcttccgc cgggagcctc ggggcagacg ctcagaagca acgggacggc gtggattccg
     6541 aacgatgctc tgacgagcga cggggtgaac gtgaccgttg cagggaaact cgagatgggc
     6601 atctccccgc gtgccacggc gggaagcctg gattttctgc acggagatag tgccaccggg
     6661 aatgtcggac tcgggttcga ctcgctgccg gtcggggcct cggggatttc caacacggct
     6721 tatggaacaa gttccctgac ggcgctgagc agcggagcgg agaacaccgg ggtgggttcc
     6781 ggggccttga attcgaactc gactggaggc gccaatagcg cggtcggatc ggcggcgctt
     6841 tccgcgaata cctcggggaa cttcaatacg gcgatgggat tcttcagtct tcgttcgaac
     6901 accacggcgt cctacaacac ggccatcggc agggggacgc ttttcctgaa cacgacgggt
     6961 gcgagcaacg tcgccatagg ggacatggcg atggcctcaa atacctcggg acccacgaac
     7021 gtcgcggtgg gctcgggcgc actccagaac aacacggtag ggtcaggcaa tgtcgcgctc
     7081 ggatctgcag ctgggttgaa cgttgtgtca ggcagcaaca acgtcttcct cagtcaccag
     7141 ggagtcgctg gggacaacgg tgttgtccgc atcgggaccg cagggagtca gacggcgacc
     7201 ttcatcgcgg gcatccgggg cacgacgact gcgcttaacg acgcgctgcc ggtggtgatc
     7261 gactccgccg gccagctcgg gacgatctcg ccgacggtca tggccaacgt ccctgtcatg
     7321 gtcctgatcg agcttcagaa catgaggcgc agggccgagg ccgctgaggc cggcctcagg
     7381 gcgctggagc aacgaatggc gaagctggag gcagcggggg cgagagccga acgctaggcc
     7441 cgcggccgga cgcccgaggc cggcttcgcc ggcgccggcg cctcgcggac cgcgcgctcg
     7501 agcgcggact gccagggcgc gaggtcgatc ccggcgttcg cgagcttctc gttcgacagc
     7561 gccgagaaga gcgggcgcgc cgtgcggagg cccgagcgct cgagcgaagc ggggatcacg
     7621 ttggacgggc tcgcggcgag gcgcacgatc tcgagcgcga tctcgtgcca cgtcgcgaac
     7681 cccgagttca cgcagtggta gatccccgcc gtcgccttct tctcgacgag ccggcgcgtc
     7741 gcggccgcca cgtcgtccac gtggacgggg gagaccttgc ggtcgtagaa cgccgtgatc
     7801 gtccggccct gcttcgcctc cgcgatcatc ttgtcgatcg ttcccttgct cccgggcgtc
     7861 ccgaagaggg cctcgacccg gatcacgtac ccgcggggaa cgttctgcgc gtagatctcg
     7921 ccgaggagct tcgtcgcgcc gtagacgttc acggggttcg gcttgtccgt ctcgcggtac
     7981 ggctgggtcg ccgtgccgtc gaacacgtag tcggacgagt actggacgag cgccgcgttc
     8041 gcggagcggg cgacgtccgc gaggctccgg accgcgaacg cgttcacggc gagcgccagt
     8101 ttcgggtcgt cctcggaagc gtcgacgccg aggacggtcg cgttcacgat gacctcgggc
     8161 tggagcttcg cgacgcggtc gaggaccgcg aaccgctgcg tcacgtcgag cgcggcgcgg
     8221 gtcagggcgg cgacttcgtg gcccgtcttt cgcagctcgc gcacgagcgc ctgtccgagc
     8281 tgccccccgg cgcccacgac gacgatcctg agtgccatac acccctactg tagcgcagga
     8341 gaacggcttc ggccgtatgc tgggccgccg atgagcgcct gccccgaggt cgccgaagcc
     8401 gtcgccgcgc tgcgcgccgc gggcgtcctg accgccgagg agggcgagcg gccggggcgg
     8461 gtggcacggg gcgagctggt gtcggtccgt gccgagctgc gtgccgcgtt ctacctcgga
     8521 gtcctgctcc tcgtctcggg cgtcggactc ttcctgaagg agaacgtcga ccggatcggc
     8581 ccatcggcga tcgccggagc tgtcggcctc gcggcgggcg cgtgtctcgc gtgggcctgg
     8641 cggaaggccg cgcctccgac gtggggcgag gccgctccct cgcatcccgc gttcgactac
     8701 gtcctcctcc tcggcgcgct cctcgcggcc gccgacctcg cgtgggtgga agcgcagacg
     8761 cgcctgctcg gccccgcgtg gccgctccat ctcctcgtcg tggcgctctt ctacggcgcc
     8821 ctcgcgtacc ggttcgactc gcggatgctc ctctcgctct cgctcgcgtc cttcgcggcg
     8881 tggcgcggcc ttgcgctcag cgtcgcgcac gcgtcgctcg gcccggggga cgtcgtgcgg
     8941 ctccggtggg aagcgctcgc gacgggcgca ctcttcgcgg ccgtcggcgt cgcgacggcc
     9001 agcgcggaga agaaggccca cttcggggac gtctgggtaa acgccggcgc cctcctcgtc
     9061 ttcggcggcc tcctgtcggg cgtcttcgga tcggagctcg actggggggt gtggctcgtc
     9121 gcgctcctcg gcgtttcggc cgccgtcgcg gcggcggcgt accgatggaa gcgcacgctg
     9181 ccgttcgcgc aggccgttct cgcggcgtac ctcgggtcga tgcgcgccgt gttctcgcag
     9241 ggtctgggag gaggaggctt cgaggcacgg ttccttctca tggcgatgct cggcggggcc
     9301 gcgctcgcga cgatcttctt cgcccaccgc cgcatgagga ttccttgaaa agggaatggg
     9361 tcgcggctga gcgcgtcgaa gaggtccgcg ccgcggcccg cggctggaaa agagcgggcg
     9421 ggatcggcga gggcgcgttc gaagaaatct cccgccgcta tcccgagccg cggacgcttc
     9481 ccgccccgct gtggcggatc ctcaccttcg tcttcggcag cttcatcctc ctcgcgttcg
     9541 ccgcgttctt cgccctcacc gtccggccga gcggatcctc cgcgtgggtc ctgtgcgcgg
     9601 tcctcggagt cggattcgtc atcgcggccg acctgcaggc gcggtcgccc gccatggctc
     9661 tccggggagg cgtcgaggca gcgagtttct ggggcatcgt cctcctgatc ggagccctct
     9721 tccttctatt cgaagaaaat cttcatctct ccgaacccga tggcccgaat ctcgtccttc
     9781 ttggcgcggt gctcctcctt gccgtgggcg ccttccgctg gggcaacgcc gtctacgcgg
     9841 gtttcgccgc aggcgccctc ttcgtcctcc tcgcgcgtgc gccacacggg cgtctgctct
     9901 ggatcgcggg cggcgtcgcc ctgagcgccg tcgccgggcg cttcaccgac cagccctcgt
     9961 gggcgccctc gcaccgcgcc tgcgcgtggg gccttgtcgt ctgcgggatc gcggcggtct
    10021 acgcggccgt gaacttctgg tcgctcgacc accggacgat cgagcaactc ggaaggcacg
    10081 cgcggtccct gccggaatcc tggccgggcg acggcgcgcg tgccgcggcc atcctcgcga
    10141 ccgccctcgt gccgctcgcg gtgcttctgc gcggcgtcgt gcggcgccgg aagatcctcc
    10201 tcgacacggg cctcgtcctc cttgccctct cgctcgtgac gctgcgcgcg tacgtccacg
    10261 ccctcgacgt gcgcgtggtc ctcgcggggg cgggagcgct cctcatcggc ggcgcgctcg
    10321 cgctgaaccg ctggctctcc cgcggctccg gcggcgagcg gcggggcttc accgcggatc
    10381 ccctgttcgg ggacgaaacg gccttccgtg ccgtcgagct cgcgcccgtc ctggccgccc
    10441 acgcgccggc cgcgaagccc gccgacgagc cgggattctc ggggggcggc ggcggtttcg
    10501 gcggaggagg cgcaggtact tcgtactgaa tgacttgaga ccctgattca tctgctctct
    10561 ggcggaaaag ttgcggaggc cgtcaaattt cgtgccggag ggtcctccat ggccagtccg
    10621 gcccccgttc gcgaacgacc gaagcccttc ctcctcgagg agttctgcaa aggctgcggc
    10681 cgttgcatcg aagcgtgcgc caagcactgc atcgaagtgg gccacgagat caacccgctc
    10741 acgggcctcg tgcccgtcgt gctgaacctc gaccattgca acgcctgcgg cctgtgcatc
    10801 gacgcgtgcc ccgagcccta cgggctccgg cccgaggcga aggcgaagag cggcgaggat
    10861 ttcgaactgc aggacccggc caagctcttc ggcgacaagc tctccgcgcg gccgaagccc
    10921 gtggcgattc cgcccgagat cgtccctctc ccgaagaccg agccgctcgt gctgaagggc
    10981 aactacgcgg ccgcggtcgg cgcgctcctc gcgggctgcc ggcacgtcta cggctacccg
    11041 atcacgccct cgacggaagg cgccgagctg atggcgaagc tgctgccgcg cctcgacggc
    11101 gtgttcctgc aggccgtcag cgaggtcgcg accgtcaaca tgatgtacgg ctgcggcggc
    11161 gcgggcctcc cgtgcatgac gttcacgtcc tcgccgggct tctcgctgat gctcgaaggc
    11221 atctcctaca tgatcgggtc cgaggtcccg ggcgtcttcg tcgacgtcat gcgcggcggt
    11281 ccgggcctcg ggaacatcgc gcccgagcag tccgacgtga agctcgcctg ccgcggcctc
    11341 ggccacggca acacgcacgc gatcgtcctc gcggcctcga cgccgcagga gatgctcgac
    11401 ctctcgcgcc tcgccttcga gctctccttc aagtaccgca accccgtgat cctcctcgcg
    11461 gacggctacc tcggccagat gaccggaaag gtcgtcctcc ccgaggcgat ggtcaagccg
    11521 ggccttcccg agtgggccgt cttcggcgac gcggcgcacc gcggcaacct gatctcgtcg
    11581 atctacctcg acgagaagga cctcgagacc ttcaacgtcc acctcaacga gaagtacgcg
    11641 cgcatcgagg ccgccgagca gcgcgccgat ctcttcctct gcgacgacgc ggacgtcctc
    11701 gtcgtctcga tcggcacgcc cgcccgcatg gcgaagggcg ccgtcgaggc gctgaggcgg
    11761 cagggcgtca aggccgggct cttccggccc gtctccgtgt ggccgttccc gatcgacgcg
    11821 ctgaagccgc tcctcccgaa ggcgcggcgc gtcgtcgtcg tcgaggcgtc ggcgggccag
    11881 ctcgaggacg agctcaggct cgccctctcg cacgccggcc tcgcgtgtcc cccgatcgag
    11941 tccgtccgcc acatgggcgg cgtcctgcct caggcctccg aggtcgtgaa ggcggtccag
    12001 gcccgcgagg aggtgaccgc gtgagcgtct tctacgagaa cttcgagcgg cactccggcg
    12061 gccagggcct caaggcgcac gcgacgcact actgcccggg ctgcggccac ggcctcgcgc
    12121 acaagttcct ggccgaggcc atcgaggagc tcggcgtcca ggaccgcatg gtcgcggtct
    12181 cgcccgtcgg ctgctccgtc tttctctatt actacttcga cgtcggcaac acgcaggccg
    12241 cccacggccg cgccccggcc gtcgcgatcg gccacaagct cgcgaacccg gagtcgatcg
    12301 tcgtgagcta ccagggcgac ggcgacctcg cgtcgatcgg cctcgcggag atcgtctcga
    12361 cggcgcagct cggcatcccg atcacggtca tcttcgtgaa caacgcgatc tacggcatga
    12421 cgggcggcca gatggcgccg acgacgctca tgggccagaa gagctcgacg agcccggacg
    12481 gccgctcggc cttcatgggc cagccgatga agatggccga gctcatcgcc gggctggacg
    12541 ggcccgtgta cgtcgagcgg gtggccctgt acgacgcgaa acagcgcatc aaggccaaga
    12601 aggcgatcac gaagggcctc cggatgcagg tcgagggccg cgggttcgcc ttcgtcgagg
    12661 tcctcgcgga gtgcccgacg cacctcgggc tgacgcccga ggagacggaa cactgggtca
    12721 aggagtccat gctcccggtc ttcccgctcg gcgtgaagaa ggacgtcgtc gtcgagccgc
    12781 acgcgaaggc gcccgccccg aacttcgacg gcgcgcgtgt cgcgagcctc gtcggcgcct
    12841 ccgcggagaa gccgccccgc ttcgcgaagg gcttccccga gcacctcggc ggcagggacg
    12901 tcgcgctcaa gctcgcgggc gcgggcggcg acggcgcaca gacggccgcg atgctcatcg
    12961 cgcgggccgc gatctccgag ggcttcgacg cgacgcacat cccgagctac ggccccgagt
    13021 cacgcggcgg cacgtcctac gcggacgtcc acgtggccga ggacgaggtc ctctccccgg
    13081 cctcgccgca cccgcacatc ctcgtcgcct tcaacgcgcc gagcctcgcg aagttcgcgc
    13141 cggacgtcgt gcccggcggg acgatcgtgt acgacagctc ggtcatccac gacgtcccga
    13201 aggtcgcgga cggcgtgaag ctcgtcggcg ttcccttcac cgagatcgcg gtcgacctcg
    13261 gcaaggcgat ggtcaagaac gtcgtcgccc tcggcgcgct gtgcgccgcg acgggcctct
    13321 tcccggagga cacgttccgg acggcgctca agcaggccct gaaggacaag tgcgcgctca
    13381 tcccgctcaa cgaagaagcg ttcgcctggg gcgtaaagtc cgtcacgacg cccggccact
    13441 gacggagggg gacatggaac acaacacgac gcacagcctg acgcctgccg aatggcgcct
    13501 cgtgaacgcg ctgcgcgaag tccccgagtc cgccctgagg acgcgcgtga acgcgctcgt
    13561 cgagggcctc atcgaactcg cccgcgagcc gaagtgcccc gagcacatgg cggacggcgt
    13621 gccctgcgcg agcgttcacg cgaactgcga ccagtgcgtc caggtgaccc gtatgctgga
    13681 aggcctcgag gcgatgcagc tccagatgcg gggatacccg ggcgcatgac gccgagggcc
    13741 ctcctcgagc acgaggtcta cgcgctcctg aagtccgcgg gcttcgaggt gccgaggcac
    13801 atcttcttgc ctggcgtgcc ggagaaagcc gaaaggcagt tggaagattt cttagaaggg
    13861 ctagggaagg aagacgctgc gggcgaagtc gtcctcaaga tcgcctcgcc ggatctcgcc
    13921 cacaagtcgg acgtcggcgg actctcactt tcgaagaagt cttcctcttc cgttctcgct
    13981 gccgcgaaga agatctggga cgacgtcgcc ctgcgtgctc ccggcgcgga gcgcctcggc
    14041 atcctcgtcg tcgagaaact cgtccccgcc tccggcactc cggccgccga agcactcctc
    14101 tcgtacaaga acgacgccgc gttcggtccg gttctcgtct tcgggctcgg aggccttctc
    14161 accgagtggt acggaagtct cgccgcgggt cacacgaccg cgatcctcag accgggggaa
    14221 gtgaagcaag gcctccaggc cgcggtcgag aagtctccgg cgctgaagat tttcttcgaa
    14281 ggtaatagag cccgagcgaa ggcgcccctc gagctcgatg cggtggcctt gctgctggaa
    14341 acactttcta agaatcttct cttctctttc tccccctcga actctctcgg caatcccact
    14401 ctcgaagaac tcgaagtgaa tcccatcttg ctgtgtgcgg acgggcgctg ggtggccgca
    14461 gacggcaagg cgcgtttctc gtcgcgacgc gcggaacgcg cgtcgcgccc ccttggcaag
    14521 atctcttctc ttctctctcc ccgcagcgcc gtcgtcatcg gcgcatccgc gtccggcccg
    14581 aaccccggcc gcatcatcct gaagaacctc aagcagtccg aaggcgtcgc gtacgggaag
    14641 ctctgggccg tccacccgaa ggagaagacg atcgacggcg ttccgtgcgt cgcgtccgtg
    14701 gcggacctcc cgcagaaggt cgacctcgcg gtcgtgtccg tgccggcgcc cgccgcgccc
    14761 ggcgtcgtcg ccgcgctcgc ggacggaaaa gccgagtcga tcatcctgat ccccgggggg
    14821 ttcggcgagg cgggccgcgg cgacctcgag gcggcgctcc gcaaggcgct cgccgacggc
    14881 cacgcgcgcc ccgacggcgg gccggtcctc gtgggcggca actgtctcgg gatcgtctcg
    14941 aaacgccagt acaacacgtt cttcctgccg cagtacaagc tgccgttcca cgacgcgccc
    15001 ggcgactcgc tcgtcgcggt gtcgcagagc ggcgcgtacc tcgtgtccct gacgagcaac
    15061 ctcgacggca tcgtcttccc gcgcgcgtcc atctcgtacg gcaaccagat ggacctcacg
    15121 gcggcggact tcctcacgta cttcgccgac gatccgagcg tccgcctcct cgtcttctac
    15181 gtcgagggct tcgcgcccgg cgacggcgag cgcttcgtgg aagcggcgcg gcgcgtcgcc
    15241 gcccaaggac gtcgggtcgt cgtcttcaag gcggggcaga cgccgctcgg cgccgaggcc
    15301 gccaagagcc acacggcctc gctcgcgggc gactacgccg tcgcgaaggc gctcctcgag
    15361 ggcgccggcg tccaggtcgc acagacgctc gacatgttcg aggactacac gaaggtccac
    15421 acgatgctcg ccggccggcc cgccgccggg cgccgcctcg cggtgctctc gaacgcgggc
    15481 ttcgagtgct cggccgtcct cgacaagctc tacggcctcg tgccttcggc cctgatgccc
    15541 gcgacgctcg cgcgcctcaa ggcgtgcctg cccgggatcg cccacgcggc gaacccggtg
    15601 gacgcgacgc cgatggccga cacgcccgcg ttcgtcgagg cggcggcggc gatgctcgac
    15661 gacccgggag tggacatgct cctcgtctcg ccgattccgg tcacgcccgc gctcgacatc
    15721 ctcgcgccgg acccgttcgg cgcgcacgcg gagaacctgt tctcgaagtc gtccctcgcg
    15781 caggagctcc tgcgcctctt ccgcgcgacg cagaagcccg tcgtcgtgaa cgtcgactcg
    15841 ggccgcctct acgacgactt cgtgatggtc ctccagcgcg gcggcatccc ggtcttccgc
    15901 aagatcgacc gcgcctcgcg cgcgctcgcg gcgctctgcc gggcctgatt cgtcagcgaa
    15961 tcgctcccgc cgtcttcatc ttcctcgtca cgttcgccag cgtcagctcc agcatgagtt
    16021 cgcggctcat ggacacgttg ggagcgaaat tgcggatgaa ggcgatctct tcgtccgtcg
    16081 gccgcggcgt cacggaaaga tcaggggcga ccttcaggtc ccagggcacg tcccgtttca
    16141 cgtcctccac ggtgaccttc gggtgaacct gcgccaggta gatctccttg gtcacggggt
    16201 cgaatcgaaa gacgcccttc gtggtcagca ccatggaagg gccggatccc ttcgggaaga
    16261 ggccggactt ctcgcggctg tccccaccgt cgagatgtcc gggcgacgtg aagtaatcca
    16321 gcttgttcac gaaggttccg cccagcatcg tgaggacggt tctcttcgcg taggagaaga
    16381 agtcgggggc gccccccgag cccggcagac gaagggtcgg cttgtcgtag tcgccgatca
    16441 cggtcgtgtt gacgttgccg aacttgtcga tctgtccgac gccgagaaag cagacgtcca
    16501 cgaagccgcg gtaggtcatc gtgaaggtgg aaaacaggtc cgtgccgtac gagaagcccc
    16561 ggcacgcgtg tgcgtcggcg atgtggttca ggggcagcag gggctgaaag tcgacgatcc
    16621 cggactccat catgagcgtc gcgttgggcg cgtagagcgc tttggccagt gcgccggcgg
    16681 tcgtcgggat cccgatgccg aggatcacgt tttcgtgatc gtggatttcc ttggcggcgg
    16741 cgatgatcaa cagctcctgc agggtgtacg gcttactcat agagctgccc cctctctttc
    16801 aggatcgtct ccatttcggg cagggtgagt cccgtgaaga tctcccggcc gtcgcggtcc
    16861 cagcgggacg tcacggccgc gccgtagttc gccggcgcgg agtagtaggg cttcgccttg
    16921 agggaatcga gcgcgccgct gccgaacttg gcgacgtacc ggtcgatgta ggccgcgcgg
    16981 ttctcacagc cgtacaccca ttcgtccatc caggccttca gcccgtcggc ggtggaatcg
    17041 gcctgcgtga acacgccgta catcgcggaa tcgaggttgt agtagccgag cacctcggtg
    17101 ggatgcgcgc cccagggaac ctccaccacg gcgttgacgc gataggcggg aatcagggtg
    17161 agggccgggc tcgactgaac gatctcgtgg tcaacgatct cttcgcagga gacgatgatg
    17221 tgcttgctcg cgagcgcggc cgccgcgatg cttcccagcg cgccccagta ttgggcgttg
    17281 ccgaacttgt cggcacgctg cacgtggacg atgcagacgt cgggattggc cgcgggcacg
    17341 gtcaggattt tcttgccggt gtacgggcac tcgatgacgc gaaacttgtt ttcgcccatg
    17401 aagccccttt tccggaagag gtcggtggcc atgacccctt cgaggaccgg catgaaggtc
    17461 atgccgtgca tgccggcgac cagcctcgcg ttgtagtcga agttcgtgta gtcctcgagc
    17521 tgcagggtcc ccgccttcag cgcccgttcc gccgcagagc cgcccttctt gccgccggcg
    17581 cgcatcacgt acgccgtgac gagtttgctc acgcagcccg cgccgatgag gatttccacg
    17641 tcgaaaatgc cggattgcga aaagagcgcc aggcccttct tctcttgccg ggccacctcg
    17701 tagaccagtt ccgcgcagga accgacggtg tagtttccga tcaccagctg gtcgccgtcg
    17761 tggacgaatc ggctgatggc gtccttgacg ctcatgacct tgctgtttgc ttccgtcaca
    17821 acatcctccc ggttcgcttc gaaggtcgag tcgaatggag cttgactcgc gattccgatt
    17881 acgggctaat atataagtgt accggtgggc cggtcaagtt atagaaaacc accggataga
    17941 cgagacgcgc gggcgccggc tcacccggtg tggaatggcc gacggcgcca gctgaaagga
    18001 aagaagagtg agcattcgat tcgatggacg agtcgcgatc gtgacggggg cgggcggcgg
    18061 cctcggaagg tgctacgcgc tggagctcgc gcggcggggc gccaaggtga tcgtgaacga
    18121 cctgggcggg gctctggacg gtacgagtgg ttccgcggga ctggctcagg cggtggcgga
    18181 cgagatcacg gcggccggcg gaatcgccgt cgcgaaccat gacagcgtgg cggaccggtc
    18241 ggcggcgcag agaatggtgg acgaggccgc gagccgcttc ggatcggtgg acattctcgt
    18301 caacaacgcc ggcattcttc gcgacaagac gttcgcgaag ctggaactcg acgacttcca
    18361 gaaggtcgtg gacgtccacc tcatgggcag cgtcaacatg accaaggcgg cgttcccgct
    18421 catgaaggac aagggctacg gacgcatcgt cttcacgacg tcctcctcgg gcctgtacgg
    18481 gaacttcggt cagacgaact acgccgccgc aaagctgggc gtcgtcggtt tcatgaacac
    18541 gctcaagctc gaagcgaaga agtacggcat caaggtcaac tgcatcgcgc cgaccgcggc
    18601 gacccggatg acgggcggcc tgtggcctcc cgaggtgctc aacgccgtgc cgcccgagcg
    18661 cgtcgtgccg gccgtcgtct atctctgcag cgagggcagc ccgaacggcc acatcatcga
    18721 ggccggcggg tcctacttcg cccgagtcgc catcgtcgag gcccgcggcg tcgtcctggg
    18781 cccgaacgcc accgctgagg acgtcgcggc gcggtacgag gagatcgccg acctcaccgg
    18841 cgcgcggacc ttcgactcgg gaatggaagt cgccacgaac atcgcgaaag ccgccgcgga
    18901 ggccttcccg gcggccgtga cgtcctagtc ttggcgtgac ctcggagccg gccgccctgc
    18961 aatgctcgtt cggaggagga gatccaacgt gtcagacccc agccgtttcc agaactcgcg
    19021 tccgttggaa aaaccgtacg ccgtttggcg gtccgccgcg ggcgcggtgc tcgtgatcgg
    19081 gtccctcgcc tgctcgaccg ctccgcccgc ttctccgcaa gcctcggcgc ctgcggcgcc
    19141 gaccgtcgtc gcgaacggcg agcgtctcgt cggcgagtac gtcggcaagg gcggctccga
    19201 ggcggtcttc cgggggattc cttacgcggc gcctcccgtc ggagagctgc gctggaaggc
    19261 gccggcgccg atcacgccgc ggccgggcgt gcagcaggcc acgaagttcg gcccgtcctg
    19321 cgtccaggaa gtcgagggca actacttcta ccggtacatc gccgagacgc tcggccagga
    19381 cccgaaactg gttccgctcc ccgggccaag cagcgagaac tgcctgttcc tcaacgtttg
    19441 gactgcgaac ctgggcggca aggacctcaa gcccgtgatg gtctggattc acggcggcgc
    19501 gaacgccgtc ggaacggccg tggggcttca gaccgacgga gcgaacctcg cgcgcaaggg
    19561 agtggtggtc gtctcgttca actaccgcct caacctgttc ggcttcctcg cccacccggc
    19621 gctgaccgcc gagtccggac acggctcgtc cggcaactac gcgctcctcg accagatcgc
    19681 cgcgctgaag tgggtgcagc agaacgcggc ggccttcggc ggggacccgg ggcgggtgac
    19741 ggtcttcggc gagtcggccg gcggcaccaa catcacctac ctcctggcaa cgccgctggc
    19801 gcgcggtctc ttccaccgcg ccgtgatcga gagtgggggc tacgcggtga gcgacttccg
    19861 cccgctcggc gaggcggagg cgttgggcaa gagcttcgag gaggcgctcg gagtggcggg
    19921 gtcgaaggac gtcctcgcgg ccatgcgcgc agtgtccccg gaggatctcc gccgcgcctg
    19981 gctccgcacc cggaagctca cgggtaacgc gcccaacgtg gacggctggg tgtttccgag
    20041 ggcgaccgcg aagatcttcg accggggcga gcagcacaag gtgcccgtcc tcatcggctt
    20101 caacaaggac gagtggacga ccctgcgcca atactggccc aaccccaccc tcgacgactt
    20161 ccgaaaggta atacgggccg tctggggccc gttcgccgat caggtgctca agctctaccc
    20221 ggcgacgacg gacgccgagg cggccgcggc ggtggaccgt tatagccccg actggtacta
    20281 cgtcggcccg acgcggttca ttgcggagcg gacgacgcgc gcgggcagcc cggcctacgt
    20341 gtacctcttc agccgcgtgc cgcaggcgcc gggaggcgag aagctcggag cccaccacgc
    20401 tatggagatt ccgtacgtgt gggacaccct caacgcgatc ccgtggatgc cgcggaaacc
    20461 ctacgaccat gagctcgccg gcgcgatgag cgcggccttc gtgcgcttcg cgatcaccgg
    20521 cgatccgaac ggaggcaaac tgcctgcgtg gcctcgctac aagagtgggt cggaggactt
    20581 cctcgagttc ggcgacacga tccgcgccag ctccggcatc cgtcccgagt tctgcgacct
    20641 ctacgagaag ctgctggcgt cgtgggcgac ggcggcccgg ccctgacggc gtcgagaccc
    20701 tgaagggacg agcgtggacg cgaacaccct gagcacgctg ggcatcgtcg tctccctagt
    20761 cttcatcatc acgctcgccc tgcgcgggtg gcacatcatc gtcctcgcgc cgctggcggc
    20821 catcatcgcc gcgctgttct cgaatctgaa cgtcctcgag accctcaccg ggccgtacat
    20881 gaccgggttc acgaactatg cccggcgctt ctatctcatc ttcctgatgg gcacgctctt
    20941 cgggaagttc atggaagaca gcggcgcggc ccgctccatc gcccaggcga tcctgaggct
    21001 catcgggggc agcgctgaga cgccgctgcg cgtcctcgtg gccttcgccc tggttcccat
    21061 ggccttgacg tacggcggcg tgagtctctt cgtcgtcatc ttcacggtgc tccccatcgc
    21121 gcggcatctc tggcaggaag cggacatccc ctggcatctc ttcatgctgt cgttcgtctt
    21181 tggcgcggcg tccatgacga tgacgatgct cccggggacg ccgcagatcc agaacctcat
    21241 gccgacgaag tacatgagct cgacgcccgc ctcggcgccg gtgctcggtc tcctcggcgc
    21301 gttcgtcgtc ttcgcgatca acgtcctcta catgaggtat tgcgtacgca aattcaaacg
    21361 ggaaggggag cactacgttc cgccggccgg cgctgcgggc ccggcggccc ccgagcgggg
    21421 gacgccgcat gccgggctga gcctgctccc gatggccgcc gtcctgatct tcctgaacgt
    21481 ccccgccgtg aaagtggacg tgatctacgc gctcggcatc ggcatcctcg tcacgatgct
    21541 cctcttctgg cgtttcattc cgaacgtcgt cgacacgatc acccggggcg ccgcgaacac
    21601 ggtcgtcccg atcgtcaaca cgtgcgccga cgtcgggtac gggatggcca tcgcggcgac
    21661 ggccggattc cgcgtcgtgt ccgcctggct cctgaccctt ccgatgcatc cgatcgtctc
    21721 gctctcgatc gccacgaaca tcatggcggc gatcacgggc tccgcctccg gcggcatggg
    21781 aatcgtcctc gagacgctgg cgccgaaata cctggcgctg ggcctcagcc cgcatctggt
    21841 ccaccggatc gtggtcatgt ccgcggggtg cttcgatgcg atgccgcaca atggcgccgt
    21901 gatcacgatg ctcgcggtcg ccgggctgac tcatcgccag gcctacaagc acatcttcgt
    21961 cggccacgtc ctcgcgacgc tcgtcgccct gatcgtcgtg attccgctgg gcatcctgct
    22021 gtactgagcg tgtgtcgacc atgagccggc tcaaggacaa ggtctgcgtc atcaccggcg
    22081 ccacgagcgg catcggcaag cgcaccgcgg agatcttcgc ggccgaggga gcgaagctgg
    22141 tgatcgcggg acggcgcgag gccgagggcc gggcgatcgc gagagccctg ggcggagcct
    22201 gcgacttcgt gagaaccgac gtcacgctcg aggcggaggt ggaagcgctg atcgcgttcg
    22261 cgctggagaa gcacgggcgc atcgactgcc tgttcaacaa cgccggcggg ccggggccgg
    22321 tcggcggcat cgaggagatt ccggtcgcgg gcttcgacgc ggcgatggcc gtgctggtgc
    22381 gctcggtcgt gctcgggatg aagcacgcgg cgcccgcgat gctgaggcag aaatcgggca
    22441 gcatcatcaa caacggctcc gtggccgcgc atcgcgcggg ctattcctcg tccctggttt
    22501 attccgcggc caaggccgcg gtcgtccacc tgacgcgctg cgtcgcgatg cagctcgggg
    22561 agggcggcgt gcgggtgaac tcgatttcgc ccggcttcat cccgaccggc attttcggga
    22621 aggcgttggg aatggcggcg gaagaggccg agaggtcggc ggagacggtc aagtccggtc
    22681 tcggcagggt gcagcccatt ccgcgggccg ggctgaccga cgacgtcgcc ctggcggccg
    22741 tgttcctcgc cagcgacgac tcgaccttcg tgaacggcca cgacctggtg gtcgacggcg
    22801 gcatgatcgg cggccggggt tggacgcagc atcaggaaac gctgcgcggc ctgaagcgcg
    22861 ccttcggcct cgacggctga gcccctccgg ctacttctgg aactggtagg cgccgcccca
    22921 ggactttccg aacgccttgg ccttgaagat ccgggcgccg cggccgccga agtctccgga
    22981 tccgatgatc agctcgtcgg tcccgggaag gatcgccatc gtggtggtcg cgagcatcgc
    23041 gcccttgtcg cttcccggta gcaggacctg gccgatcagg ttgccgagcg cgttgaagac
    23101 aatgaccttg ccggcgccga aatacgcgac gtagacgttg tcgtcggcgt cgatcatcgc
    23161 cgagtcgggc ccgccgaacc cgttgaaacg gtacggaatc atgtggccgt agaggttcat
    23221 cgtgacgccg tcggctccga ggtcgaggcg gtgcagctgc tgatggaacg tctccgtgac
    23281 ccagagcgtc ttcccattcc tgcccagcgc gaccccgttg ggggaggcca ggttcttgag
    23341 aaccggcgtg atcgtcttga agtccggaga gacgtagtac accgcgccat tcgcgacgtt
    23401 ggcggcgtcc gtgtagtcct tgaaatccgt gaaatagaag ccgcccttgc cgtcgaacac
    23461 catgtcgtcc ggcgtgaagc gcccggccgg gacgatgacc tccatcccgg tgccgtcggg
    23521 ccgcatcgcg acgatccggc cggtggcgtc tccgcgataa ccggccttct cgcccagatc
    23581 gcagaggaac aggcgcccgt ccttgtggat cttgaccgtg gcgaaccggc tcttcgacgg
    23641 cccgtacacg gtcgtcaccg tcttgtacgg aggagcggac cggagaacgc tgccgttgcc
    23701 cacgaggacc atgtagagat tgccgtcacg gtcgaagatc agaccttcgg gcgtctccgc
    23761 ggcgttcgtg acgagcagcc agtcttcggc gacgacggtc ggtagctccg cccgtccgac
    23821 ttcagcggca cccagaagga cgagagccaa gaatgatgcg agggtcttca tcgggtgcct
    23881 cctcccggag tcatcgtttc accgcctcct cgatcctgcg acccgtggcc gccgccgcgc
    23941 gcgcctctgc cgcgcggccc ctgcggttca gcaggtcggc gagaacgtag tagccgagag
    24001 gtccttccgc atgcccggga tcgagcgcga ttccttcccg ggccagattc tcggcgcgca
    24061 cgagatcgcc gcccgtgtcc atcagcagtt tggcgaagta gtagcgcccc tggacggaac
    24121 cggggttgga ccggagaaac gcttcccacg actgcctggc ccgctccacg tcacccatcg
    24181 cggcgtggag ccggccgagg ttgaactgcg cccggaagaa cgccggttcg agctcgaggg
    24241 cccgcgcgta ctgctcggcg gcgagtcgac cctcgccgcg tttctcgcga agaaagccga
    24301 ggttgaagtg ggggagggcg aagccgtcgt cgagggcgac cgcgcgttcg aactgaggca
    24361 cggcctcgtc gtcacggccc tgctcgacgc gcacttcccc catcttgttg gcgaagaaag
    24421 gcgccgattc accgctctgg gaggccttat cgagcacgtc ggcggcctcg tcgaaccgct
    24481 tgagcctgac gtagacgtcg gcgatggcga gcgaggcgcg cgcgtccgcg cccttgagct
    24541 cgaggacccg ccggaagccg gcgagcgcct cgtcccagcg gccgagatcg gcgtactcgg
    24601 cggccaggtc gaacagcgat tgttcgtgca tggggttcag ctcgagggcc cgcttgaaat
    24661 ggcggatggc gtcccgcggg cggcggcgct cggccgcgat ctggcccagc atctggtgcg
    24721 cgtcgacgat ctccgcgtcc tctgcgagga cctgctccag gagtcgtccg gcggcgtcgt
    24781 attcccggtg ggagatccgg ctctgggcat ccacgatctt ctggtggagc tgcaggcggt
    24841 ccttgggatc ggcgcggacc cgctcgcttt ccttcccgac ggcgacgcca cccccgccgg
    24901 ccaggtagcc gagtgaccgc agccgggcga cggcgcgctg gtcgatctcg ggtgcgttgc
    24961 cggcagggag cttcccgctc tcgatgcggt ggcgcagtct gccgaggtcg gcggtcaact
    25021 cccgagctcg attcggatcc acgctgtgaa ggttctgctc ctcggtggga tcctgcgcga
    25081 cgtcgtacag ctcgggccgc gggacgtcga tgagcttgaa acggccggcg cggagggcac
    25141 ggaggggggc ccatccgtag tgcaacagcg ggtacaggga ctcggagtag accggtcgat
    25201 cccagggtac gtcccggccg gcgacgagag gcgcgaggct ccgtccgtgg acgttctcgg
    25261 gcgccttcag ccccacgagg tcgaggatcg tgggcaggag gtcgacgtga ctcaccgtcc
    25321 ggtcgacaac gcgccctccg cggacgcctc cgggaggcct gacgatcagc gcggcgtgga
    25381 tcgtcgtgtc gtagacgagg aagccgtgga acgtctcgcc gtggtcgccg agcccctcgc
    25441 cgtggtcact cgtgaggatg acgagcgagt ccttgaggag gtggcgctcg tcgagggcgc
    25501 gccggaagtc gccgacgagc gagtccgtat aggccaccac ggcgtcgtac ggccgtcccg
    25561 gataacggga cttgaacggc tccttcggaa cgtacggatc gtgggggtca aagaggtgga
    25621 gccagaggaa gaaggggcgg ccggccggtc tcttgtcgag ccagttcagt gcggccgcga
    25681 cggtatcggc gccgtcgcgc cgaaccagac ccacgttcgc cacgttcgcc gtccccagcc
    25741 cgccgagatc gaaatcgtcg aagtagcggt cgaagccgcg gccgatcccc catcggtggt
    25801 cgagcacgaa cgcgctcacg aagccgcccg tggtccatcc gctcttgccg agcagttccg
    25861 cgagcgtggg gatgcgcccg tcgagaacgg tgcctacgtt ctcgcggacg ccatggccgg
    25921 gcgggtagag gcccgtcatg atcgacgcgt gcgccgggag ggtgaggggc accgtgctcg
    25981 cggcgttcgt gaagcggacg ccctcggccg cgaacgcgtc gatgttcggc gtgtcgacga
    26041 tcctcgagcc atagcacgag agacggtcgg accggaaagt gtcgagggtg atgagaatga
    26101 cgttggaggc ggccgcccgg tgcgccgtcg gcctggaacc acgcagaaag aggaaggcga
    26161 gagtggcgct cgcgatcacc cccgcggcga cggcggcgag gaccgcccag cggcgtcgtc
    26221 cgctcaatcc ggcagcacgg cgtgcatggc gagctcggtg atttcgtccg ccatctgcgc
    26281 ggccgacagt ctgcccggct gtcgatacca cttgggcagc cacaggacca tgccggccag
    26341 cgtgtgagtg gcggcggtga tgtcgacgtt gcgcaacttg ccggccgccg cgagccgctc
    26401 caggcgctcg cgaatgaggc cgaggtagaa gcgcttgcgg cgggcgactc gctcccggtc
    26461 ggccggcagc agcgaccgca tttcgtccac ggcgaggatc gcgaaagccg cgtcgtcctc
    26521 gcgggtcatc atgacggcgt gggagtggat cagacggcgc aggtcgtcgg cggcgtccgc
    26581 ggcgctgtcg gtgagctggt tccggatcac gtcctcgtgg atgtcctggg cgtagctgat
    26641 gatcgcggcg agcagctcct gcttgctgtt gaagtggtag taaagcccgg ctttgctgat
    26701 ctcgcaggcg cgtgcgacgt ccaccagcga gatcatctcg aatccctgct ccaggatcag
    26761 gtgtgcggcg cgccgcagga tctcgtcgcg ccggctcgcc cgacgccggg gtcgagcggg
    26821 ggttgccgtc gtcgtttccg gtctctgtcg agccttcaaa tcgggccttc cttcctgcct
    26881 cgatttccct tgacaagtac gcgtttgagg gtatcagata ttaaaactat accgggaggc
    26941 cggtcaactt atggagggca gggaggaggg accgttgatg tctaagacag cgaagtgctt
    27001 cattctaagt gtcctgttgc tcgcatccgc cagcggattc gctcagatta ccggatcgat
    27061 ccacggggaa gtgatcgatt cggagggcca gccgctcccg ggcgccacgg tgaagctcac
    27121 aggtgacccg atcccgggag ccgagcgcat cgctacgacg gacgcgaagg gagccttccg
    27181 atacggcgcc ctgcccgtcg gccggtatgc ggtgtctgcc gcggtcccgg gcttcaagac
    27241 gcaacaggtg aaggacgtcc gggtgtcgat cgacgccgtg gcctccgtga ggatccggat
    27301 gcagctgcaa accgtctcgg aaagcctgac cgtgaccgcc acggcaccgc tggtggacgt
    27361 tgtcagctcg ggcgtggccg tcaactacga ctcccagttc gtccagtccc ttcccacgcg
    27421 caacaacttc accgacatca tcgcggtggc tccgaccgtg agcgcgccca acgagggaaa
    27481 ccccttgctc gcggcgtacg gcggcaacgt cacgtcccag cagtggaaca tcgacggcct
    27541 caacctggcg tcccccgagg gcggctggct gggctggttg atcaacccgg agatcgttca
    27601 ggagacctcg ctcaagggct tcggggccgg ggcggagtac ggcagcacgg cgggcaacgt
    27661 ctacaacatg gtcacgaagt ccgggacgaa cgccttccac ggctcggccg gcggctactg
    27721 gacgaacaac tccctcaccg gttcgaacgt caagctcgac ccgtcgacgc tctattccta
    27781 ccgcctctgg gatcctgcgg gcacgtacac catcgacgac tactacgacg cccgtgcgac
    27841 cctcgggggg ccgattctcc gcgacaagct ctggttcttc ggcgccggcc agtgggacaa
    27901 gatcaacatc gtgggtccca acggcgtcgc ggggatcgac ggctccggca cgacggacta
    27961 ccgctacgac gggaagctct cctggcagat cgccgatggc catcacgtcg acgttcgcgg
    28021 acacacggcg aaccagaaga ccgtacccgc gccggacatg tacacggcgc tttcggccgt
    28081 cgtgacgtac gacatcgaca tcaacatgat cacgggggac tacaccggga tcctggctcc
    28141 cagcacgctc ctcaaccttc gcggcgggta ctggagcaag aaccaggaca tggcctcgcg
    28201 cacgggcagc aacgaagagt ggctccagga ggccatctac ccgggcccgg ccctcaacaa
    28261 aggcgggatc ttctggttca gcggccgcaa ggagaactac acgcaggccg acgcggtcct
    28321 gtcgcagttc gcgagcgact tcatcaaggg gagccacgag ttcaagttcg gcgtccagta
    28381 caacgagggc gccggccaga ggaacgcagc caagtccacc ttccggtgga ggcagccgcc
    28441 gagcccgggc ttcgcggaca agtactggga attccggtac cagatcctgc cgcccctcat
    28501 ctacggggcc gagaccacga cgtggggggc gttcctgacg gattcgtgga agatcagcaa
    28561 ccggctgacg ctcgacttgg gcgtgcgctt cgacgatcag aagggcaaga ttccgtccta
    28621 cccgaagctc gacctcgccg gaaacccgac ggacgtgatg ctcccgtccg cggacatgat
    28681 ccactggagt aatttcgcgc cgcgcatcgg cttcgcctgg cagccgacga aggacggaag
    28741 gtccgtgctt cgggggttct acggacgctt ctgggacggg ccggtctcgt ccgcgtggta
    28801 ttacccgccg ccgggacggg gcaactcgga agtctggttc gtttatccgt accagttccg
    28861 cgtctcgtcc gtggcggccc cgccgcccga ccagctgctc gcccccgggg tcaagaaccc
    28921 cttctcggat cagttcgcgg tgtcctacga ccaccagatc ggcagcgaat tcgccgtcgg
    28981 ggcgcagttc atctacaagc acaccgacga catgatcggc tggcagatcg aaaacgacgg
    29041 cgtgtgcaag cagttcctgt gggacgaccc ctggacgacg aacgtcaagg agcagatccc
    29101 gctctgcgag ctcaccaaga acccgacgct tcacaagggc aacggacccg ggcccgggtc
    29161 actcgcgccc ggcgacacct accacatgta ctaccggggc gccgtcctga ccttcaagaa
    29221 gcgctacagc aagggctggg acctgatggc gtcctacacc tattcgaaga cggaggggat
    29281 caaccccagg cctcactaca acggctccct gggtcaggga ttgcctgtgt tcagcagcga
    29341 cgaggggtcc gatcccaacg actggtacaa cgcctaccac ctgcaggagg gcgaccggac
    29401 ccacatgttc cgggtccagt cgaacgtcga tctcggctgg ggcctccgag cgagcggcgt
    29461 cttcaactgg cagagcggcc gaccctacca gcggctcgcc aacgtcatcg ggccgacgac
    29521 cggcacggtc atcacggtca cggcggacgc gagcgacagc ctccggctgc cgagccaggc
    29581 cattctcgac ctcggcctgc agaagacctt caatttgggc aagggcgtga acctggacct
    29641 cggcctgcag cttctgaacg cactcaacga cgacgccgtg gagtactact cgtcgatggt
    29701 gctcttcccg ggccagaact tcgagccgtc ggcctgggtc ggcccgagaa ggctggtgct
    29761 ccgggcccag gtcacgttct gagataacgg atggaggcgc ggtgcgacgc cgcgcctcct
    29821 ccctgcggag atcaacgtgc cgaacaaagt cgtcatcgtc aaagcggtgc gcacgccgat
    29881 cggtgccatc ggaggaggcc tggccggttt ccaggcccag cagattgcca cccacgccat
    29941 caaggctctg atcggctcgg ccggaatcga cccggctgca gtcgagtaca cgtgcatggg
    30001 atgggtcatg caggatccgc gctgccccaa cgtggccaag accgccgcgg aactggcagg
    30061 cgtgccgccg accgtgcccg ggaccaccta ccaggaaaac tgcgcctccg gcggcgctgc
    30121 gatccacagc ctgtgcaggc gcatcctgct cggggaggtc tcgctcggca tcgccggcgg
    30181 ggtcgagtcc atgagcaacg tgcctcgtta cctctttgcc ggccgcacga agaaccagat
    30241 gtacggcgac atgaccctgg tcgacggcct gttcggcgcc cttaccgacg cgtacgtggg
    30301 cagcggcgag ctgatgggcc tcctcaccga gcgcctggtg gcgaagtacg gcgtgacgcg
    30361 ggaagagcag gaccaggtgg cctaccgcag ccaccatgcg gccgtcgacg cgtgggagaa
    30421 gggagccttc gcggactacg tggtgcccgt cgaagtgcct cagaagcgcg gcgcaccggc
    30481 cgtcgtggcc cgggacgagg ggccgcggcc ggtgacccac gagcagctgg cggctgccaa
    30541 gccgtacttc aagcgcgacg gcggcacgat cacctcggcc aacgcctcga cactcaacga
    30601 cggagccgcg gtggtgctgc tcgccagcga ggagcgcgcc cgcgagctcg ggctcgagcc
    30661 cctcgccgag atcgtcgcgt atcacaacgt cggcgtcgag cgtgagctca tgggcgaggg
    30721 agcattcaag gtcattcccc cgctcctgaa gcgagccggc gtcgaccttt ccgaggtgga
    30781 ctacctcgag atcaacgaag cgttcgcggc agtcgtcgcc gcggcgtacc acttcctgcc
    30841 cgggctcaag gccgagcgca cgaaccagtg gggcagcggc atctccctcg ggcacccggt
    30901 gggggcgacg ggcgcgcgtc aggtcgtgga catggtccac cagctgcgtc ggaggggcgc
    30961 cgcgctcggc gtcacgagca ggtgcgtcgg cggcggcatg ggcagcggcg aggtgcttcg
    31021 gcggctgtag caaccgaccc tgattcatct gcttcgttcc gccttccggc ccggcggcta
    31081 agatctcgtc tcaaggaggc cgcccgtgag ccagggaaac cagcccgtcg tcgcgatgct
    31141 cagtcctgcc gagatggagc tgatccgcct gctccgcgag ccttccacgt ctcccgtgcg
    31201 cgcgcgcgtc aagaccgtcg tcgagggcct cctcatggcg gcgaaggagc cgttctgccc
    31261 cgaaaagccc tgtccgcagg cgccggggga gtgcgaccgc tgcgtgcaga cgatgcgcgt
    31321 cctcgagggc ctcgaggcga tggagatgca gatcaagggc ctgcccggcg cctgaggtaa
    31381 cctcgcctcc ttcggacagg aggcccccgt gcgcactgcg tccctgcgcc tcgtcctcgc
    31441 cgccatgacg atcgccacca ccaacacccc cgcccagacg gcatcggttc cgcccactcc
    31501 accagtggcc aagaaggtgg agaaggtcca cctcctgcac ggcgacccgc ggcccgaccc
    31561 gtacttctgg ctgcgggaga agtcgaaccc tcaggtcgcg gcctacctcg aggccgagaa
    31621 cgcgtacacc gacgcggtcc tcaagccgac ggagccgctc cagaaggcgc tctacgacga
    31681 gatgctctcc cacgtgaagg agacggacct gtccgtcccc taccgcgacg gcgcgttcct
    31741 ctactactac cgcacggaga aggggaagca gtaccggatc cactgccgga agcccgcggc
    31801 ggacggcgcc gagcaggtcc tcctcgacct gaacgcgctc gccgagggga agaagttcat
    31861 gtccctcggc gcgttccgcc cctcggacga cgggaacctc ctcgcgtact cgacggacga
    31921 cacgggcttc cgccagtaca cgctcgccgt gaaggacctg aagacgggcg cgctcctgcc
    31981 cgagcggatg ctgcgcgtca cgagcatcgc gtgggcggcg gacgggaaga cgctcttcta
    32041 ctcggtcgag gacgagacga cgaagcgctc gcaccgcgtc taccggcacc gggtcggaac
    32101 gcccgcgtcc gccgacgccc tgatctacga ggagaaggac gaacggttca acgtcggcgt
    32161 cggccgcacg aggagccgcg cgtggatcgt gatggagagc tccagccaca cgacctcgga
    32221 ggcccgcctg ctgaaggccg acgcgcccga gggcgagtgg acgctcgtcg ccccgcgcga
    32281 gcaggaccac gagtactacg tcgagcaccg gggcgacctc ttctacatcc ggacgaactc
    32341 cggcgggcgc aacttccggc tcgtgacggc gcccgtcgcc gcgccgggac gcgcgaactg
    32401 gaaggagctc gtcccgcacc gcgccgacgt catgctgacg cggatcgacc tcttcaagga
    32461 ccacctcgtc ctcgaggagc gggcgaacgg gctgccgcgg ttcaccgtga ggagcttcgc
    32521 gacgggcgac tcccacgtcg tgtcgttccc ggagcccgtc tacaccgcga gctccgagac
    32581 caaccgcgtc tgggacacga aggtcttccg ctacggctac cagtcgttcg tgacgccgca
    32641 gtccgtcttc gactacgaca tggacgcgcg gaaggcgacg ctcctgaagg agtgggaggt
    32701 cccgggctac gaccggacgc actatgcgtc ggcgcggctc tgggcgacgg ccgcggacgg
    32761 cacgaagatc ccgctctcgg tcgtctggcg ctcgaagacg gccgagggca aggcccgcac
    32821 gctcaaggac gggccgttcc cgacgctcct ctacggctac ggctcgtacg gcatcccgat
    32881 cggcgtctcg ttccggtccg accggctccc gctcctcgac cggggcttcg tctacgcgat
    32941 cgcgcacatc cgcggcggcg gcgagatggg gaaggcctgg cacgacgccg ggcgcatgaa
    33001 gaacaagatg aacacgttca cggacttcat cgcggccggc gagcatctcg tcgccgaaaa
    33061 gatcgccgcg aaggaccgcc tcgccgccga gggcggcagc gcgggcgggc tcctcatggg
    33121 cgccgtcgtg aacatgcggc ccgacctctt ctcggcggtc atcgcgcacg tgccgttcgt
    33181 cgacgtcctg aacacgatga gcgacgccac gctgccgctc acggtcggcg agtacgagga
    33241 gtggggcaat ccggcgaaga aggacgagta cgacttcatc aagacgtact gcccgtacac
    33301 gaacctcagg aagggcgcgt acccggcgat gctcgtcaag acgtcgttca acgacagcca
    33361 ggtcttctac cacgagcccg cgaagtacgt cgcgcgcctg cgcacgctga agacggacgc
    33421 gaacccgctc ctcctcaaga ccaacatggc cgccgggcac ggcggcgcgt cgggccggta
    33481 cgacttcctg cacgagattg cgctcgacga ggcgttcctc ctgacgcggt tcggtctggc
    33541 gaagtaggcc ggcgcgccgt gtccgccgag gaccacgcga acgtccccgt gatcccgccc
    33601 gtcctcttcg ggctcgggat cggcgcggga tacctcatgc ggtggctcgc cccggtccgg
    33661 ctcgtcccgc tgcgcctcga gcgggaggcg gtttgggcgg gagcggcgct ctcgctcctc
    33721 ggcctcgcgt tcggcggctg ggcgttcttg acgttcgtgc gcgcgaggac gacgccgcat
    33781 ccgaaccatc cggtgagcgc gctcgtgacg tgggggccgt accgcgtctc ccgcaacccg
    33841 atgtacgtcg gcctttcggg cttcgttgcg ggcctcgcgc tcgtcgcgaa cacgccgtgg
    33901 ctcctcgccg tccttccgtt cgtgtggctc gcgctccggc gcctcgtgat cgaccgcgag
    33961 gaggcctacc tgacccgcag gttcggggag gaataccgcg cgttcctcgg ccgcacgcgc
    34021 cgctggctct gaacggtccg gctcaggatg cgcgcaggcc ctccacgatg ggccgccgcg
    34081 cggccatcac ggccgggacg atcgccgaga agacgcccac ggcgagcgcg acgagacccg
    34141 cgagcgcgac gacctcgggg aagatcttca tcccggccgc gaacggagcc atccgcgtct
    34201 ccatgagcgc gcccttgagg cgggagaacg ccgcgaggaa cgcgaggaac ccgagaaccc
    34261 cgccggctcc tgcgagcgcg aggctctcgc tcatgacgag gcccacgatc cgcggccggg
    34321 agaagccgag cgtcctgagg accgcgacct cgacgacgcg ctcgcgcacg ttgagagcga
    34381 ccgtgttcgc cgcgatcacg acgacgacgc cgatgatgat gacgcccatc gcggtcagga
    34441 gaagctggac gttcccgagc agcgagacga accccagcgt gaaggcgttc tcgctctcgg
    34501 tcttcgtcgg gttcggcgag ttctcgaaga acgcgtccac ctccttcggc agccgctcgg
    34561 cgcaggccgc gtccgtgcag cgggtccaga gggtcgagac ggtgccgcgg aagggcggcc
    34621 aggcctcttc gaggtacgcg cggtggaaga agatgctcgc cgagttctcg tacgggaggc
    34681 tgtagacgcc ctggatcgtg aacgtgaggt cgacggggta gagcgtgccc gtgatcgtca
    34741 cgcggtcgcc gaccctccag ccgtacttct tcacgagggc gacgccgacg aggccacccg
    34801 tccggtcgcg tttccacgcg tccgcggagc cctgcacgat cttcgcgtcg tcgaagacgt
    34861 cgagaaaggt ctgggggtcg acggcgaagc gcggaaagac gttccccgcg ctcgcgtcgc
    34921 ggtagaggcc ggcgaagcgg tcgatcgggg tgatcgcgcg gacgccctcg agcgcggcga
    34981 tccggttcac gaccgcgtac gggagggacg tcgtgaggcc gaccttgtgc cgcgtcacga
    35041 gacggtagag gccgcgctcg cgcgcggggt cggggaggtt gagcgtcctg aggaacgtcg
    35101 ccatgaacgc gacgaggaag acgggcagca ggatcgaccc gacggtgagg acggtccgcg
    35161 tcttcttgcg cagcaggttc ttgaggacga acggcaggaa gcgaagcacg gtcagcaggc
    35221 tttcggggcg tcctccgtca ggatacgcgc cccggattcc gtcacgagga cgtcgtcctc
    35281 gatgcggatg ccgagcccga ggtactcctt cgggacgcgc gtctcgtcgg gccgcacgta
    35341 aaggcccggc tcgacggtca gcaccatgcc cggctccaga gaccgcggcc gcccgcgctc
    35401 gtcgcggtat cggccgcggt cgtgcacgtc gaggccgagc cagtgcgacg tgtggtggag
    35461 cgcgaagcgc tgtgcggcgt tcttcttctt gagcgcctcg cgccggccct tgaggaggcc
    35521 gaggtgcagc agcgcgtcga ggaggacggc tcgtgccgcc tcgtgcggcg cgtcccacgg
    35581 tgcgccggga cgcacagcgg cgatcgcggc gcgctgggcg gcgagaacgg cgccgtagag
    35641 cgccttctgg ggtcgcgtga accgtcccga agccgggaac gtccgcgtca cgtcggaggc
    35701 gtagccgccg aactcgcagc cggcgtccac gaggacgagg tcgcccgcgg cgaggcgcct
    35761 cgagttctcg acgtagtgga ggatcgtcgc gttctctccc gaggcgacga tcgtcggata
    35821 ggcggggccg ctcgcgccca tggcccggaa tcgccggtcg acggccgcct cgatctcgta
    35881 ctcgtaccgg ccgggccgcg cggtccgctg cgcgtcccgg tgcgcggagg cggcgatcgc
    35941 ggccgcccgc tcgaggagcg cgatctcctc gggcgacttc aggaggcgca tcgcgtggag
    36001 gaggtccgtc gggtcggaga tcgtcacggg gccccgtttc gggtggcgtg cctcgcggcg
    36061 gaagcgcgcg aggaggccgg cgacgagccg gtcgttcgcg cccgagaggc cgagggcgtg
    36121 gtggaggacg cgcgccttgc ggacgagatc ggggaggcgc ctttcgcgct ccgcggcggg
    36181 aaacgcctgg tccgctccga acgtcttcac ggcgccctcg ggcccggccc ggcggccctc
    36241 ccacgtttcg cgctcgcggt cccgcgggag gacgaacagc gtgaaggtcc tcgcgtccgc
    36301 gtcgaggacc gcggtcgcgc ccggttcggc gaagcccgtc aggtagaaga ggtcgggatc
    36361 ggggcggtag cggtagtgga cgtcgtgccc gaagggggct tcgggggttg cgaagaggat
    36421 cgcgacgcct tctttcatcg cgtcgaagaa gcgcgcgcgg cggcgcgcga agacggtccg
    36481 gtcgtccatg gggcgagtct aaccggcgcg gcatgcgacg atggggcatg gactcccgca
    36541 tcgtcccggc gctccgggcc cgctatcccg agttttcggg cgcccggctg aagcgcgccg
    36601 tcctggaagg gcaggtcacg gtggacggcg ccgtcgtgaa cgaccccggt gcctttgtcg
    36661 ccgccggagc ggaagtccgg tgggaccgcg accgccgcat cgagcgccgc gtcgcgacgg
    36721 ccctgaaggt tctgtacgag gacgacgacg cgatcgcggt cttgaagccg tcgggcctcc
    36781 tcacgcaccc gaccgaggcg aaggagaagg acacgctgct ggcgcgggtc tccacctacg
    36841 tccagaagag gcacggcggc ggcgcgcgga cgtacgtgtc catcgtgcac cggctcgaca
    36901 aggagacctc cggcatcctc gtcttcgcgc gcagccgtcg cggcctgatc gggctgcagg
    36961 cgcagctccg cgcgcacacg atggaccggc gataccgggc ggtcgtcgag ggcgacctcg
    37021 tcggggacgc gggcacgttc gacaaggacc tcgttgccga gccgggtgag cggcgccgag
    37081 gcgtcgtgaa ggccggcgag aagggcctga aggcgatcac ggaatggacg gtgctcgagc
    37141 gcttcgggat cgcgacgctc gtcgaggcgc gcctgaagac cggtcgcacg caccagatcc
    37201 gcattcactt cgcgaacgcg gggcacgtcc tcgtcggcga ctccgtctac cgcgaccgca
    37261 aggccaagcc gttccccgtc cccttcccgc gccaggcgct ccacgccggc cacctcgggt
    37321 ggaagaccga ggaaggcaag gcggtctcgc tggacgcgga acctcccggc gacttcctca
    37381 agctgctgga tgatttgagg aagaggggga agaagagaag atcttcttag aaggtgtggg
    37441 agcgggcgca gcgcgccgtc gcggctgtgc ggtgactcgg gcgcgcagcg caaacacacc
    37501 cttctaagaa aacagcgggc ccgaagggcc cgctgcagaa tcctcgttct tcggtcccgg
    37561 tttcccggtc ttcggctatt cgaggacgac gatcacgacg cggcggttct tggagcggcc
    37621 gacggccgtc ttgttgctct cgacgggaag ggtgtcgccg taggagatcg tcgacatgcg
    37681 gccgagcggg agcttgtgct ggcgggcgag gtagcggcgg acggcctcgg cgcgctgctg
    37741 gccgagctgg tcgttgtagt ccgtgccgcc cgtgtcgtcc gtgtggccct ggatctcgag
    37801 gtacacgccc ttgtcgagcg cgaggacctt caccgcgaac tcgtcgagag cggacttcgc
    37861 ttccttcgtc agctcgaagg aattgacctt gaagcggaca cggtcctcgg cgaacgactg
    37921 ctggaagacg atcttgccct tcgcgaggac gcgggcttcc tgggcgcgcg cgagcgcgtc
    37981 cttggcggac tggccgagct gctcgatctg cttgccctgc tcgtcgatct tgccgccctg
    38041 ggccttctgg ccttcctgca gctgctcgac ctgcgtttcg accgactcga tcttcttgtc
    38101 ggtcttctgc tgcgactcgg cgatcttttt gtcgatgtcg gtcgtcgtcg cacaaccgac
    38161 gagcgacagg ccgacggcgg ccgcgaggag agtgacggga accttcatga gtcctccttg
    38221 ttcgttgttc agccgccctt gatgggtggc agtgtgcccc gatagcccgc cctcacgcgg
    38281 acgggtcgtg cggggcccga aagtttcagc gagatgcgcc ggtatcggac cggcccggcg
    38341 cccgtgggcg tgaagcccac gagatactgc gtgcgcaggt ccttttcgac gcgcgcgatc
    38401 gcggccggca gctgggcggc ctcgtcgacg atcgagagga tcccgccgga gaggcgcgcg
    38461 acgtgcccga ggacgtccag gttcagcatc gcctcgggtg tgattccggg cggcggcgtc
    38521 acccgcgcgc cgggcgagcg caggccgatc gggtagacgg ggacgtccac gccctcgagg
    38581 agcgtcgcga gatcgtcccg ggtgagctcg gacgcgttgt cgatcccgtc ggtgagaagg
    38641 aggatcgcgc gcgatccgtt ccgcccgagg agcgtgcggt cgggcatccg ggcgagcgcg
    38701 tcgtagaacg cggtgcgccc gtgaggcgtc acggcatcca tcgccgcgag gagcttcgcc
    38761 gtgtccgcgg tgaagggcac gacctcgcgg acggcacccg aggagaaggc gtagagcgaa
    38821 aagtcgtctc ccggcagctt ctgggcgacg agcgcgcgca cggcggcgcg cgccccctcc
    38881 atctttccca cgaggcccat cgagcccgag gcgtcgagca ggatcgtgaa cgagacgggg
    38941 gcatcgtcgc tgcgggagaa gagatccgtc aggacgggct ggccgtccac gaggagcgtg
    39001 acgtccttct cctgcaggtc cgcgaccggc tttcccttca ggtccgtgag gacgaagggc
    39061 accattacgt agccgacgga caccgaactg gtcgtttttg ccggcttgga gacctgcgcg
    39121 gacaccccgg cgcccagcat caaggtgggc gcgaggagca tctggtgcag cggaagtctc
    39181 tgtatcagca tagtttcggg cgccggatct gatctttcca aggggaagtg cggaggagcg
    39241 ccggcgcctt tctacaacgc gaaaagcaaa ggggccgcca gcggcggccc cgaacatccg
    39301 gaagtgtgac gcgcgttaca tctcgcgggc ggaggtctca gaggaggtcg atggccggga
    39361 agaagaacgt cacctcgcgg gcggcgtcgg cggggttcgc cgagccgtgg acggcgttgc
    39421 gctcgatcga ctggccgaac tccttgcgga tcgtgccggc gtcggccttg cccgggtccg
    39481 tcgcgcccat cgtggcgcgc cagtgggcga tggcgcccgc gcgctcgagg acggcgacgt
    39541 agacgggccc cgacgtcatg taggcgacga gggcggagaa gaagcccttg gccgcgtgct
    39601 ctccgtaaaa cgactcggct tcggccttcg tgatgttgtg gcgtttgaag gcgacgaccc
    39661 ggaagccctc ggcttcgatg cgcgagagga tcttcccggc gttcttcgat tcgacggcgt
    39721 ccggcttgat gatcgcgaat gttttttcca tgcggccggg gagtatacgc gggagcacgg
    39781 gcgcttgcct tcgcccgcgg tccttgcata ggctcgcggc aatgaagaat ttcttcgagg
    39841 tgtcgaaccg ccgggggctc ctcgaccgcc tcgacgggct gcggccggac gcggctcacc
    39901 tctgggggaa gatggacgtc gcgcagatgt gtgcgcactg cgcgatcgcg ctggaggtcg
    39961 ccgccggcga cgtgccgaag acgcaggcct tcatcggcaa ggtgctcgcc ccgttcgtca
    40021 agaagaagtt ccttcatgga acggagcccc tctcgaagaa ctctccgaca gacccgacgt
    40081 tcgtcgtctc cgactcgcgg gacttcgcac gggagaaggc gcggctcgtc gagattgcga
    40141 agcgcttcgg cgaagccggc gccgccgcgg cggacgggcg ggtgcactcc ttcttcggcc
    40201 gcctgacggg cgaagagtgg ggcgtcctca tgtacaagca cctcgaccac cacctgaggc
    40261 agttcggggc gtagcgggcg ggcgcccact tccattgaat ccaaaacgga tccataatgg
    40321 atgcgggagg cggccatggc gacgctcacc ctgaagaacg ttcccgaaga gctcgtggat
    40381 cagctcaaag aagaagcgaa acagaaccgg cggagcctga atcaggaggc gctctcccgc
    40441 ctcgaaagca cgatcgcgcg tcggagacgc tcctcggagg aggtcatcgc ggacctccgc
    40501 cgcctccata aacggctcgg ctcccgtccg atcatgacgg acgcgctgat cgagcgagcg
    40561 aagcgggaag ggcggccgtg atcgtcgtcg atacgaacct gatcgcttac gccgtcgtgc
    40621 ctggcgaacg aacggaggcc gcgctcgcgg ttctggattt cgactcgcag tgggtcgcgc
    40681 cgcgtctttg gcggtccgaa ctccgcaacg ccctggtcac gacgatgcgc gcgggcaagc
    40741 tgagtctttc cggagccctg ggtgccttcg aggaggctga tcgtctcgtt acggataccg
    40801 acctggaaga ggagacggag gcgtgcctga ggctcgcgga gaaagggggg atctccgcct
    40861 acgacgccga gttcgtcgtc gtggcggagc gcctcggaat gccgctggtg agctcggatc
    40921 ggaggctcgc gaaggccttc ccgggccgcg tcctctcacc cgaggagttc gtggcccgcg
    40981 gctgaatccg tcaacgaaaa gggcgcccga aggcgcccct gacgattcca cttccggagc
    41041 cgagggctac ttgccgagcg ccttcttcag cgtctcgccc atgagagcgg gggagtcggc
    41101 gacgtggacg ccggccgcct tgagcgcctc gaccttttcg ccggccgtgc ccttgccgcc
    41161 cgagatgatc gcgcccgcat ggcccatgcg gcgcccggga ggcgccgtgc ggcccgcgat
    41221 gaacgacacg acgggcttgc cgaggtgcgc cttgatccac gcggcggcct cttcctcggc
    41281 gctgcccccg atctcgccga tcatgatgac ggcctgcgtc tcgggatcgt cgcggaagag
    41341 ctcgagcgcg tcgatgaagt tcgtgccgtt gatcgggtcg ccgccgatgc cgatgcaggt
    41401 cgactgcccg aagccgagcg acgtggtctg gccgacggcc tcgtacgtga gcgtccccga
    41461 gcgggagacg acgccgacga acccggcctt gtggatgcgc gccggcatga tgccgatctt
    41521 gaccttggcg gacgggcgga tgatgccggg gcagttcgga ccgatgagcc ggctcgtgcc
    41581 cctggcgagg tattccttcg ccttgatcat gtcgttgacg gggacgccct ccgtgatcgc
    41641 gacgatgagc gcaacgcccg cgtcggacgc ctccatgatc gcgtccgcgc aggccggcgg
    41701 cggcacgaag accatcgagg cgttcgcgcc cgtcgccttg acggcggcgg cgaccgagtc
    41761 gaacacgggg atgccgtcga cggccgttcc gcccttgccc ggcgtgacgc ccgcgacgac
    41821 cttcgtgccg taatcgcggc tcgcgagcgc gtggaagcgg ccctcgcggc ccgtgaggcc
    41881 ctgcacgacg agtttcgtat ccggcgagat ccagttcgca cccatgtcag gccgccttcc
    41941 cgagcgacgc gatgaccttc tcggccgcgt ccttcatgcc gtcggcgggg atgaccgcga
    42001 ggccggagct cttgaggatc gccttcccct cctcgacgtt cgtgccctcg aggcgcacga
    42061 cgacgggaac cttgatgccg atctccttga ccgcgttcac gacgccggtc gcgatgatgt
    42121 cgcaccgcat gatgccgccg aagatgttga tgaggacggc cttcacgttc gcgtcggaca
    42181 ggatgatccg catcgcgttc ttgacttgct cctcgttcgc gccgccgccg acgtccagga
    42241 agttcgcggg cttgccgccc gcgtactgga tgatgtccat cgtcgccatc gcgaggccgg
    42301 cgccgttcac catgcagccg acgttgccgt cgagcttgat gtagttgagc ccgaacttcg
    42361 aggcctcgac ctcgagagga tcctcctcgt cgaggtcgcg gtactcgcgg atcgccgtgt
    42421 ggcggaagag cgcgttgtcg tcgaagttca tcttcgcgtc gagcgcgagg acgtcgcccg
    42481 cccccgtgat gaggagcgga ttgatttcgg cgagcgacgc gtcggtcgcg acgtacgcct
    42541 tcgcgagcgc gatcaggaag ccgacggcct tcttgtgcgt ctcgcccgtg aggccgagcg
    42601 cgtccgcgag cttgcgcgcc tggtagggct ggaggcccca cgacggctcg aagggctcct
    42661 tgacgatcgc ggaggggtcc ttcgcggcga cgacctcgat ctccatgccg ccctgcttgc
    42721 tcaccatcat gacgggcttg ccgatcgcgc ggtcgagcgt gatcccgatg tagagctcct
    42781 gcgcgatgtc cagggcctcc tcgacgagga ccttcttgac cttctgcccg gccggccccg
    42841 tctggtgcgt cacgagctgc atcccgagga tcttccgggc gagggcctcg gcctcctcgg
    42901 ggctcttcgc gaccttgacg ccgccgccct tgccgcggcc gcccgcgtgg atctgcgcct
    42961 tgacgacggc gcgcccgccg aactctttcg cgatccgtcc cgcctcttcc ggcgagtccg
    43021 tcacgcggcc gcggggggta gcgacgccga acccgcggag aacttctttc gcttgatatt
    43081 cgtgaatttt catggcgacc gcggacagta tcagaaggaa tcgggagcgc tccatgagta
    43141 aactgattca tgcgcttcgc actcctcctg ggcctcctgg ccgccgcccc gtcggccttc
    43201 gcccagtcct ccgcgcgcgg cggcaggtcg gaggacgtct cggtcctccc ggcggcggcg
    43261 gcggccgacg tcgcccggca ggtcgcctcc gagctcaagc tcggggattt cgccgccctc
    43321 gagtcgcgtt tcgacgagcg catgaaagcg gctctgcccg acgcggccct gcgcgtcttc
    43381 tgggccgaca cgctgaagcg cgcgggcgcc ctgcgcgggt gcgcggagcc ccggacgtcg
    43441 ttcgcgggcg agttcacgct cgcgttcgcg gcctgcacgt tcgagaagca gaaggccgag
    43501 ctgcgcctca cgatccggcc ggacggtcgc ctcgcgggga tgttcctcgc gccggaatcc
    43561 gcggcgaggc gcgagtggac ggcgccggac tacgtgacgc ccgcggcgtt ttccgagcgc
    43621 gagacctccg tcgccgcggg actcgtgacg ctgccggcga cgctgtcgat gccgaagggc
    43681 gaggggccgt tcccgggcgt cgtcctcgtc catggctcgg ggccgcacga ccgcgacgag
    43741 accgtcggcg ggacgcgcgt cttccaggac ctcgcgcagg gcctcgcctc gcgcgggatc
    43801 gccgtcctcc ggtacgagaa gcggacgaag gcgttcgcga aggagctcgc gggcacgcgc
    43861 gacatgacgc tcaaggacga ggtcctcgac gacgcggtcg ccgcgctcgg cgtcctgcgt
    43921 gcggcgccgg gcgtcgacgc ggcgaaggtc ttcctgctcg gccacagtct cggcgggctc
    43981 ctcgctcccc ggatcgcgct cctcgacgac cggaccgccg gcgtgatcct cctcgcggcg
    44041 ccgtcgcgcc cgatgtggga agtcgtgcgg gaccagatcg aggccctctc gggcccgcgc
    44101 gcgagcgagt cgcagcgggc gacggccccg gcgctccgcc gggaggccga cgcgctcgcg
    44161 gacctctacg cgggccggcc gatgccgccg gggacggcca cgatcttcgg ggccccgccg
    44221 tcgtactggc tcgagctcaa gacccagacc ccggcgctca cgctgtccgg cctcggcctc
    44281 cccgtcctcg tcctgcaggg cgggcgcgac taccaggtga gcatgaagga cttcgcgggg
    44341 tggcagcgcg ccctcaagga cgtgtcgcgg gcggcgttcc ggtcgtaccc gaagctgaac
    44401 cacctgttcg tggcgggcga agggccgagc gcgcccgccg agtacgagaa gaccgggcac
    44461 gtggaccgcg acgtcgtgga ggacatcgcc tccttcgtga agcgcggcac gctgcgcccg
    44521 gtggagacga agaagccgaa gaagtgagcc gtcaggagcg cgcgacgagc gcgcccacga
    44581 agaacttcgg aaagcgcgcg gcgacggcgt ccacgtccgg caggacctcc acgccgcgga
    44641 atcccgcggc ggcgaaggac gcgcgccacg cggccggcga caggaagccg tggttcgggc
    44701 gcaggacggg gtccgtcctc acgtccgtga agctcacgag gaagtcgaag acgaagtcca
    44761 cgtaggacgg cggcgggtgc gcgcccggct ttacgcactc cgagacgacg acgacgccgc
    44821 ccggcttcag cgccgccttc gcctcggcga ggacgaagcc gaggtcgggc gcgacgtgga
    44881 agcagttcac ggagtagacg gcgtcgaagg agccgggggc gacgccctgc tccgcccagg
    44941 gcttcgtcat gtcgagcttt gcggcctcga cgagcgtccc gggcgcggcg gccgcgcggg
    45001 cggcgcgctc gccgcggcgc gcgaac
//
LOCUS       DAOMFY010000003        41979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_286_length_41979_cov_7.422479, whole genome shotgun sequence.
ACCESSION   DAOMFY010000003 DAOMFY010000000
VERSION     DAOMFY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 41979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41979
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_286_length_41979_cov_7.422479"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLB01_00590"
     CDS             <1..816
                     /locus_tag="PLB01_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ75823.1"
                     /translation="MLAAAGPESLTWLGHACFLVRTGGFSILTDPFLSDYASPVSGLG
                     PKRYVPPGLATEDLPDIDVVLLSHNHYDHLDGTSVEKISLKVRGERVAVVPTGLGPFF
                     ASRRYSDVRELGWGEGTLLRKSSSSVSLSCLPSIHFSGRTPFDRNETLWCSWKIVSPT
                     LSLYFAGDTGYGPVFADLGRQHGPFDVALLPIGAYEPASIMRPVHLNPEEAVAAGRDL
                     GASKLVAMHWGTIVLTDEPPFEPPARFRSAARAAGYPDERAWVMRIGETREIR"
     gene            complement(782..1414)
                     /locus_tag="PLB01_00595"
     CDS             complement(782..1414)
                     /locus_tag="PLB01_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ75824.1"
                     /translation="MDRFIADGPARAPLTLVLAHGAGAPMDSLFMNAVAAGIAKEGFR
                     VLRFEFPYMHARREGKRKPPDRGPVLEETWREAIREAAGKKGAKAVAIGGKSMGGRIA
                     SMVADEAGVAGLVCLGYPFHPPGQPDKLRVAHLKQLMTPALILQGERDAFGTREEIAR
                     YPLSKKIRISFLKDGDHSFKPRASSGRTEKQNLDEAVALVSGFLGSLRSA"
     gene            1428..2171
                     /gene="ubiE"
                     /locus_tag="PLB01_00600"
     CDS             1428..2171
                     /gene="ubiE"
                     /locus_tag="PLB01_00600"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQQ75825.1"
                     /translation="MPNKYLSYDDERGPKVRAMFSRLAARYDLLNDVMSLGLHRRWKR
                     DAVDLALAGRTGPVRVLDLCCGTGDLAFLASALAGTGSRVFGLDFTLPMLGVARERRT
                     AAAASPAFAQGDAMRLPFPAASFDAVTMGYGLRNVADPLACLKEVRRVLAPGGRVVVL
                     DFGKPSNPVARSLYGGFLMGAMPVVGWLAHGDPDTYRYIPESLERYPAQRGVRDLMER
                     AGLSSARYEDRMFGTMGLNVGEAPGRSPL"
     gene            2178..4631
                     /locus_tag="PLB01_00605"
     CDS             2178..4631
                     /locus_tag="PLB01_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQQ75826.1"
                     /translation="MPRDTLPASPEPRSGRLPGDSPAQASRLVALLVVFFVSIIAAIL
                     ATGAIGMSALSGVRAFVAAESTWSKAQKEAVYALSRYLDTDGEESHYRRFNALLAVPR
                     GDRKGRIAMSGPAPDAAAAFQGFLEGRNSPSNAEEMVSILARFRDTEHIQRAVQIWER
                     ADAVIDELEALAVVVHQDFEAGRMSAEARAAYNQRVEKLNEKAALLEDGFTAAFDQAS
                     RVFRTRLYALVAAMTLLLVGLGVAASVFVARVLKRRNSAVTASERRYRLLFERNLAGL
                     YRTTLDGKILDCNSAFARILGYPSREEVLKITALDLYSDPGDRESFVARLKQQRVLVN
                     FELCLRRRDGSPVWVLANESLMDEAGGDVVMDGSLIDITDRKRAEQQRWHQANHDALT
                     DLPNRVLFNDRLSLAILHAQRRRQSLVVMFLDLDHFKRVNDTLGHSAGDELLVKVADR
                     LRRCIRQDDTVARVGGDEFLLLLNGITREADAATMARKILGILAEPFLVQKRELFIEA
                     SIGIGLYPGDGGDAETLITNVDTAMYRAKETGRNSFQFFTRKMQEQSQERAALESGLR
                     RALPRGEFVLHYQPIYRLRDRSVVGVEALVRWRHPEKGILSPRDFIPLAEDVGLIARV
                     GEWVLRRACEQLRAWQAAGAVPMRISVNVSPRQFQQRDFAANVRRIVRESGLEPSVLD
                     LEITESIAMHDVAHTGRVLSELADLGIQISMDDFGTGHSSLNYLKHFPIRRLKIDQSF
                     VAGMTTDDKDHAIVATVVSIARNLGLAVTAEGVETEEQAALLAALACEDVQGYLFAHP
                     EPAEEVEKRLGLAAPATGA"
     gene            4699..5346
                     /locus_tag="PLB01_00610"
     CDS             4699..5346
                     /locus_tag="PLB01_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HQQ75827.1"
                     /translation="MRVDKWMTPKVITLKATDSLSAAIHLMKEKKIRRIPVVNGDGKL
                     VGIVSDRDLKDVSPSRATTLDIWELHYVLDKLKIGDIMTKKPFTVTPDTPIERAAQIM
                     MEKRVEGLPVLDAKGAMVGILTEGDVFRALVDITGVGKGRLRVDLLIPDKPGSIREVA
                     DLCRTAGGKIVSILGSYAKVPAGKRELIMRVDCPKPDVLRADLAKAYGDARVEAD"
     gene            5516..6715
                     /locus_tag="PLB01_00615"
     CDS             5516..6715
                     /locus_tag="PLB01_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ75828.1"
                     /translation="MTIRRMSSVLAVAAFAAFSASPALAQGKTFTVGVPLPLTGAEAK
                     FGEMKKQAYLMAADEINAKGGVKGTKLAFDIQDSGGKPDTASAIVEKFITINKYPIVV
                     GEYTSQCSYAVAGIAEKYKVPYLVDTGAADKITQQGWKYIFRLNSPAGLYAKGVFSFF
                     EEVAKPKTVAILFENTDFGTSTSKSMKDYAESHGMQVVLAEGYQAGAVDFKPILQKVK
                     SLRPDIVYMVSYLMDASLLMRQSKELDLNAQAFIGGAAGHTLPEFLENAGDATEFCMS
                     ASLWTPQVKYPGAATFDANFQKLYKKAPSYHAAEAYATAFVLADVLARTKSMSSDDLR
                     DALAATDMMTAFGPVKFQSWDKFTNQNKMETLVGQWQKKKFETVWPKALASAPYVYPA
                     PRWRDRK"
     gene            6743..7666
                     /locus_tag="PLB01_00620"
     CDS             6743..7666
                     /locus_tag="PLB01_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQQ75829.1"
                     /translation="MTPQVAGQVELLGQVLISGILLGGLYALIGLGMSLIMGVMKIIN
                     LAHGELMMVGMYITFWAFTLGKIDPYVSLLIVIPALFVLGAMLQKWLIAPVMEAESIL
                     PENQVLLTVGIGLVLSNLALLAFTADYRSVPVSYASKTIYWDVKMGGQTVSLSFSFAM
                     LVAFGIALVLGGALFLFLGYTDLGRMIRATSQDARAASLMGVNTKRITYITFGLGSAL
                     VGAAGALLAPVYYLFPQIGGPFTLKAFIITVLGGLGNVAGAVLGGVVLGLAESLGSVY
                     VSLGYKDAFGFIIFVLVLIFLPKGLLGKGRS"
     gene            7669..8643
                     /locus_tag="PLB01_00625"
     CDS             7669..8643
                     /locus_tag="PLB01_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQQ75830.1"
                     /translation="MSRSVLLPLAGLALLAVVPAVVSNPYVLHMLILCLLWTVLGQSW
                     NLLGGYTGQVSFGHAAFFGIGAYTTGILVKTGMSPAAAWWGLLLGGLAAAAASAVIGW
                     ICFRLRGPYFALSVLALAEVLRLVALNWKQLTNGAEGILFIPAFNSKSWYYWIVLALA
                     AASMALIRFVMHGKLGFYFLAIREDQDCAESLGINATKYKLISLLLSAFFTGVAGSFY
                     MNYMGFIDPGIVFSIHDISVMMILVTMLGGVATTWGPAVGAAIYILMSEAFRTYLKSG
                     HLVFFGILIIVIIIFFPQGIVGTLRETLQRRKRRLAAPPAGGPLKEAA"
     gene            8640..9359
                     /locus_tag="PLB01_00630"
     CDS             8640..9359
                     /locus_tag="PLB01_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ75831.1"
                     /translation="MSLLELKDVTKRFGGLQAVGHMTFTVEKGEIFGLIGPNGAGKTT
                     VFNVVNGFYAPTEGEILFKGERIDGLKPYEICRRGLARTFQVVKPLARLTVLENVMVS
                     AFARTDHRDEARAMALEATAFTEMEAWRDRTAGSLPLGMRKRLEMARALATKPELLLL
                     DENFAGLNPAEVEKTIAIVRKIHESGVTILIIEHNMRVIMSISQRLLCMCYGERIAQG
                     TPQEVANDPRVIEAYLGAAHA"
     gene            9352..10056
                     /locus_tag="PLB01_00635"
     CDS             9352..10056
                     /locus_tag="PLB01_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ75832.1"
                     /translation="MLEVKNVDVFYGDVQVLWDVSFNVQKGEIVALIGANGSGKTTSL
                     NTISSILKPKKGAVTYEGQALHTRAPYDLVGLGIAHVPEARRLFPEMTVKENLLLGAL
                     TPEAKKTRPEMLEKVYGIFPRLKEREKQAAGTMSGGEQQMCAIARGLMSKPRLLMLDE
                     PSLGLSPILVSDIFRVIQEVHDAGVTVLLVEQNVFKSLAIADRAYVLENGRIVLEGKG
                     KDLLNDAHVKEAYLGV"
     gene            complement(10031..12202)
                     /locus_tag="PLB01_00640"
     CDS             complement(10031..12202)
                     /locus_tag="PLB01_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQQ75833.1"
                     /translation="MSGARPPLVLPPTPSEASFSRRRFVRLVGGGIIVLFNADLSDLV
                     GQETRTRGYPTDFNAYLRIAADGRVTVYSGKIEMGQGVVTSLAQMAADELGVPLASVD
                     MVMGDTALCPFDNGTYGSMSTRFFGPALRAAAAEAKAVLVALASEKLQVPREALRVED
                     GAVVSTADPKVRVTFGQLANGQKIVRTLEGKAVQKSVAEFRLMGKPTKRLDARAKVTG
                     KAAYAGDIRLPGMLYAKVLRPPAHGAVMKSVDTSAAARFPGAVVVNEGGLVAVLAPDP
                     ETAEKALAAVKSDWDVPKPEFDDRTVFAHLLAVAGAGEEREKKGSLAEGEKASASLFE
                     GQYVNGYGAHAPMETHTALARLENGTMTVWNSTQTPFPNQAAIAQAIGFAKENVRVIT
                     PFVGGGFGGKSGGGLQAIEAAKLAKITGKPVQVCWSRAEEFFFDAFRPAAVVKIRSGI
                     DGAGRISLWDYQVYAAGSRGAEQFYDVPHNLMRTYGRWGSDSPKMHLFATGPWRAPGA
                     NLNVFARESHLDVMAAKAKADPLEFRLRNTSDKRMRSVLEAVAARFGWKNAAGPSGRG
                     VGIACGIDAGTYVALAAEVKVDAATGRIRVVRIACAQDMGVVINPDGATMQMEGCIMM
                     GLGYALTEELRFDGGRVLTKNFDTYEIPKFSMMPALDTVLVKNDELSPQGGGEPALVP
                     VGAAIANAVFDATGARLTEMPMTPERVKVALAGVRRPGTPP"
     gene            complement(12199..12669)
                     /locus_tag="PLB01_00645"
     CDS             complement(12199..12669)
                     /locus_tag="PLB01_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQQ75834.1"
                     /translation="MKDSVSFRLNGKPVQLDVDPERSLLWVLRTDLGLTGTKFGCGES
                     LCGACTVVIGKEAVRTCLLPVKAVRGKDVTTIEGLEKNGKLHPLQEAFADTGGLQCGF
                     CTPGMIMNAYALLLKNPKPSRPQIVAGMEDNLCRCAAHHRIVQAIEKAAAGGVS"
     gene            complement(12729..14867)
                     /locus_tag="PLB01_00650"
     CDS             complement(12729..14867)
                     /locus_tag="PLB01_00650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQQ75835.1"
                     /translation="MKRVLVFLALLAARPLGAATVTQLLGQPTDRSITLNTRADAALE
                     MNVQYGLAAGVYTATTPSVAMGADPYVGGFYVSQTVLSGLQPNTRYYYRVQTRAAGSA
                     GAFTPGTESSFQTQRAPGSSFVFCAQGDSHPEREKSMFDPALYIQTLTAVAAERPDFF
                     LTSGDDFSVDTLPTPWTQSAVTGRYTLQIPYLQIVGRSAPVFLVTGNHEQTSLWNYNL
                     PPDGTNSNQVPIWAQNARNLYYPMPAPNDPTTGTFYSGNTMTLAGISGPLRDYYAWQN
                     GDALFVVIDPYWTSPAMVDNGIGSNSGKTADRWLISHGDAQYAWLKQTLESSTAKWKF
                     VFAHHVMGTGRGGVDIADEFEWGGKSADGSYGFAVKRPTWPLPIHQLMAQNHVTIFFQ
                     GHDHLFARQEADGVTYQELPNPADYTYTAFNADAYKSGDILPNSGYVKVTVSPSSVKV
                     DYIREFLPKDESATQKSGQVAFSYTISGGSAPPPAATWILPSSARAAGQGGAFYTTDL
                     TIANTGAADASLGLKFLDHDVDGRNGAEQSFSLAAGRSVTYADVLGSVFGVSSGYGAI
                     RVASSSAALAVAGQTSTPSPGGGTFGQSVPAVGAADLVGAAPRSILGVREDAAFRTNL
                     ILANATETPLDVDVALVAETGASLGTKRYALPPLGMTQVTRVVRDLGVAADVSGARLV
                     LSTPTPGGSFAAYGSVIDAVTNDPRTLLPR"
     gene            complement(14864..16930)
                     /locus_tag="PLB01_00655"
     CDS             complement(14864..16930)
                     /locus_tag="PLB01_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQQ75836.1"
                     /translation="MAALVGLPLAADFGVPAHPVDLILGQPTGTSIVVSVVAYQDGDG
                     YVEVVPAAGGAPAATGTKALAAQQGVEFPVAGLQPDTAYSYRLLWRAGTSGAYTATPS
                     RTFRTARPAGSAFTFTLHADSHLDNNTDPALFARTMQNAAADAPDFHVDLGDTFMTDK
                     YGAAFTDAQPQYYAQRYYLGLIGASAPVFFTLGNHDGEIGYAADGTANSIAAWSARLR
                     LALFPNPSPDAFYTGNATPEPAVGLLRNYYSFAWGDAQIVVLDPFWPTREKGNGSDNW
                     NWTLGAEQYAWLKRTLESSSAKLKFVFIHHLVGGFGKDRRGGAEAAGLWEWGGANADG
                     TPGFAAHRPGWAEPLQALFVRTGVSVVFHGHDHLYVKQDLGGIVYQEVPQPGFPRADS
                     TSSAVEYGYVTGTLYGSSGHLRVRIAEGKATVDYVRSLLPADETATRHNGDVVHSYSV
                     AGKSVAPPPAATWILPSSARAPGQGGAFYSTDLALANTGAVDATYAVKFLGHDADGTA
                     GDEKTFTLAAGKGVTYADVLGSVFGVGTGYGALRVTASTTAFAVSSQTWTPAGSGGTF
                     GQSVPGFGDADLVKAGIPRSIAAVREDAAFRTNLILANAAEAPVDVDVALVSSAGAAL
                     ASKRYTLPPLGMTQVTRVVRDLGVSAETAGARLVLSTPTTNGAFAAYASVIDATTNDP
                     RTLLPR"
     gene            complement(16963..19215)
                     /locus_tag="PLB01_00660"
     CDS             complement(16963..19215)
                     /locus_tag="PLB01_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75837.1"
                     /translation="MRAALLVALLAGAAAGAPVATITAPSSVTAGQAGYAASVPAQAG
                     ASYAWTVSNGAAMVGAATESVAFSAGVSGAVALTCRVTDAGGASTTGTLSIPITPASP
                     PAFNLAQALTDGAQRTTLAFDGLGMVTGNLQAQSFFPPGKVADYWGFQYLRDNDPDDM
                     GHNTSFLTRAACDVLSILTAEQLGWLKALASTQVEPINRYAYGRYPLMQAFRRLVDGN
                     LPAGTTGLDVAAVKAASRELYVLDGQLSYDRAVVYARIYRSFTASQKAYLSAMAGKGW
                     ASWPPRTMDDVRGTTQGLTNDEVVAVMTYAGDLFSWYVGSLAGDVYFCPERHGTYFGS
                     FYMKDAPAIGHEGYSIDEQLTVTAGSALCDASKGYVTAEQAAIVSALADTQRSALYAG
                     TPSIVSARTAISTALRSLISSAEPTAGQLAAVKATVLEQSGIYGELDGANVAAYAAAF
                     ARVAATLTQAQKDRLVGLRRSFMSGRYADGTPFDFTTCATYYLYAAPADSVTLAPYVA
                     DSVRFFAAAAPAYVAAWILPSAAHAAGTGGAFYTTDLTIVNAGGVDATLALKFLGHDA
                     DGRSGTERTFALAAGKTVTYADVLGSVFGVSSGWGGVRVASTVASLAVSSQTSTPAPA
                     GGTFGQSVPACGSADLVGPGVTRSIAPVREDAAFRTNLVLANATEATLDVDVVLVSAA
                     GASLASKRYTLYPLGMTQVTRIVRDLGVAADVAGARLVLSTPTAGGALAAYASVLDAT
                     TNDPRALLPR"
     gene            complement(19212..19553)
                     /locus_tag="PLB01_00665"
     CDS             complement(19212..19553)
                     /locus_tag="PLB01_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HQQ75838.1"
                     /translation="MSPRTPSLPLRPGRVALPALALVVAAGSFLVAGHAPAQKAGGPP
                     HRPKPPLEVALDANGDGVIDADEIANASAALKKLDKNGDGRLTPDEYRPPRPAREDAP
                     PPSAEGERKSK"
     gene            complement(19639..20346)
                     /locus_tag="PLB01_00670"
     CDS             complement(19639..20346)
                     /locus_tag="PLB01_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ75839.1"
                     /translation="MSAAAPKRIAPRVLLVEDEESLVLTLTDRLVAEGYRVTARGDGE
                     SALGVAAEEPFDVVILDVMLPGVDGFAVCREMRQRGLALPILMLTARAQVVDKVVGLK
                     LGADDYLTKPFEMAELLARLEALLRRSASGSRAADVFVFGDVRADFRRAEVTRAGEVL
                     GLSSMELRLLRFFIEHRGEILSRDRLLDEVWGYEATPVSRTVDVHVASLRAKIEPNPS
                     HPVYVVTVHRLGYRFDG"
     gene            complement(20343..22046)
                     /locus_tag="PLB01_00675"
     CDS             complement(20343..22046)
                     /locus_tag="PLB01_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ75840.1"
                     /translation="MRPRKLPSLLPIALILGLLAALVALGGLQYRWIARVSEAEQEHL
                     KTVLDDAAERFCSEVDREIARVQQAFQSKDDIPDEELANDLSGRLARWRATAPAPKFV
                     RELLVVTRFGRGEIALQRFDEASGRLGDAAWEAGLESVREAFEARGKVPSLDERLPGV
                     IVPVRPSRRRGGEDERRPPPPRDPEPDAGGERRPPRQHVVVRLDREFLQKEFLLRRAE
                     REFGGPGGLAFSVSVVVAGRPDDVLFQGGPRVASREVHPEDAVRKFFTLHRPGDEPEP
                     GKWILSVRHPSGSLEAAVAGARRRNLAVSAAILALLAATLVVLVATTRRAQRLARQQM
                     EFVAGVSHELKTPLTAMRSAGQNLADGIVDSPEKVRKYGALIEREGRRLTEMVGRVLT
                     FAGIRSGVQSFRMEPVDVAALVDGVLADCRWVLEEKRVAVEKAVAGGLPGVRGDAAAL
                     RQALANLVDNALKYGGTAGWIGVGAKSVATHQGEEVVVSVADRGIGIKRSDLSRLFEP
                     FFRSADAQAAGITGSGLGLAVVRHVVEAHGGRVTVDSTPGKGSVFAIHLPADAGTGTD
                     V"
     gene            22200..23348
                     /locus_tag="PLB01_00680"
     CDS             22200..23348
                     /locus_tag="PLB01_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ75841.1"
                     /translation="MNASAEALGASSRVVEAREISALLDVILESGYRVLGPTVKDGAI
                     VYDEIASAARLPAGIGQEQTNGRYRLRKRSDGALFGYTVGPHAWKKFLFPASLRLFGV
                     TRQKKTLQVEPAAADARPDAFLGVRPCDLAAIARQDRVFLGGPYADPAYRARREKALI
                     VAVQCGEAEGTCFCSSLGTGPRAAAGFDLALTELLGDGRHQFLVEVRSDRGANLLQRI
                     DSRPARPDDLEAARAATRRAEAGMGRKLDTAGLKDLLTASNDSPRWEQIAERCLSCAN
                     CTMVCPTCFCATVEDASDLTGTSAERWRRWDSCHTLDFSYIHGGSIRRSASARYRQWM
                     THKLATWVDQFGETGCVGCGRCITWCPVGIDLTEEARVLQEARPAPAA"
     gene            23360..23836
                     /locus_tag="PLB01_00685"
     CDS             23360..23836
                     /locus_tag="PLB01_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQQ75842.1"
                     /translation="MEALDPLLAEHPFLKGLTPAHIRTVAGCARNERFDPGQFLFREG
                     EEAHQFFIVRHGKVAVEVGAPPRGAVTIQTVGDGEILGWSWLVPPYRWRFSAQAKELT
                     RVIALDGRCLRQKCEEDHDLGYELLKSFADIVGQRLDATRLQLLDVYATEPAGRHK"
     gene            23896..24708
                     /locus_tag="PLB01_00690"
     CDS             23896..24708
                     /locus_tag="PLB01_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HQQ75843.1"
                     /translation="MQPVPFVVERTQRETADTFTLELLPQGGGRFSFDPGQFNMLYVF
                     GIGEVPISISGDPAFPQRLVHTTRVVGAVTKGMGALKRGDALGVRGPFGTGWPVEEAE
                     GCDVVFVAGGIGLAPLRPALYEILAHRERFGRVVLLYGARTPDDILYRKQLHDWRGDF
                     DLDVHVTVDRATSAWRGNVGVVTTLIPRVPFDPYHTVAMVCGPETMMRFTVLELERRG
                     VPAAKVWLSLERNMKCGVGLCGHCQCGPKFICKDGPVFPCADVWDLMSRREL"
     gene            24709..25482
                     /locus_tag="PLB01_00695"
     CDS             24709..25482
                     /locus_tag="PLB01_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQQ75844.1"
                     /translation="MENAKPTLAVFKFASCDGCQLSLLDCEDELLAVAGAVSIDNFLE
                     ASRAVGKGPWDLVLVEGSITTPHDAERIHKIRRQAKFLVTIGACATAGGIQALRNFTD
                     VADYAKAVYASPQFLEVLGKSTPIRDHVFVDFELRGCPISKVQLLEVVSAFLAGRKPV
                     TPASSVCLECKRRGTPCVMVAHGTPCLGPVTQAGCDALCPSWHRGCFACFGPMESPNT
                     ASLAALWGELGVPPAALVRAFRSFNASAEAFRKESELHE"
     gene            25475..26767
                     /locus_tag="PLB01_00700"
     CDS             25475..26767
                     /locus_tag="PLB01_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQQ75845.1"
                     /translation="MSKVRTITVNALARVEGEGGLKVRVRAGKVQEVRLDIYEPPRFF
                     EAFLKGRALGEAPDLTARVCGICPVAYQMSSVHAMEAACAADPGPQVRALRRLLYCGE
                     WIESHVLHAAMLHAPDFLGYESALHMAKDHPGAVTLSLRLKKAGNEIVTAVGGREIHP
                     VNVRVGGFWRAPSPSELAPLVPKLETARGEAVELLRWASGFTFPDLDLHHELVSLRHP
                     SEYPLNEGRIVSSEGLDIAASEWEQHFEEEHVQRSNALHARRKGGGIIQTGALARYTL
                     NFDRLPAPIQAAAQEAGLGKECRNPFKSILVRCVETLWAIDEALVILRAYEPPDPPFV
                     DAVPREAVGAAATEAPRGLLFHRYRIGGDGLIKEARIVPPTAQNLRAMEADLRGFVQD
                     HLDIAEDRLTLLCEQVVRNHDPCISCATHFLKLRIERD"
     gene            26769..27215
                     /locus_tag="PLB01_00705"
     CDS             26769..27215
                     /locus_tag="PLB01_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HQQ75846.1"
                     /translation="MPLLVIGVGNDFRGDDAAGLLAARRLRACRGVRSVEHTRDGLSL
                     MEHWRPTDRVVVVDALSSGHAPGTVFRFDATEGPVPGDVGWISSHSPGVAEGIETARI
                     LGRLPASLTVYGIEAEHLDPGARVTPAVAGAVEEVALEILEDLNRP"
     gene            complement(27228..28040)
                     /gene="proC"
                     /locus_tag="PLB01_00710"
     CDS             complement(27228..28040)
                     /gene="proC"
                     /locus_tag="PLB01_00710"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQQ75847.1"
                     /translation="MLARKKIAVIGLGTMGRSIASGLLRSGKVRAQALRATARTKASA
                     EKVAKELGFACTTANTKAAADADIVLLCVKPRDVAKAVESIRGRRRRPPLVISIAAGI
                     STGFIEGHFGDATPVLRAMPNTPCLIGKGMTVLSKGSHATDADVALATEIFEPLGKVL
                     ELEEKHMDTVTGLSASGPAFIYIIIEALADGGVMRGLPRSVATTLVAQMTMGAAEMVL
                     TTGRHPAALKDDVTTPAGCTIAGILALEDGRIRSVLARGVETAAKVAGELGK"
     gene            28153..29283
                     /locus_tag="PLB01_00715"
     CDS             28153..29283
                     /locus_tag="PLB01_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ75848.1"
                     /translation="MALLALFFASVAVFADLYVTQPILPLLSHEFGVSAPTAALTVSV
                     VVLMIALVSNAYGPLADAWGRKPVMVASCAALAVPTFLCAAAPSFPVLVGLRALQGLL
                     MPGVAAVAVAYIGDAYAGAALGPAVGSYVAASALGGLTGRVASGWIASHFSWRAPFLV
                     FGAVTLVAALGMALHLPASPPGPARSVAGAFREMAGHLRDRRIAGGLVIGFTVFFGFI
                     AIFTYLPYYLTAPPFGLSTASVSGVYLVYVAGIATSWLAGRASQRFGRRAIMAAGFLV
                     AAAAVLSTLAASLPAVACGLVALCIGMFLVQGTAPAYVNAAARQAKGGAGALYTTFYY
                     LGATFGSVLPGFAWQAWGWPGVVAACLASFAAGLLAVKFLCA"
     gene            29379..30569
                     /locus_tag="PLB01_00720"
     CDS             29379..30569
                     /locus_tag="PLB01_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQQ75849.1"
                     /translation="MPICAHPAPRRLASVLATASLLALIGAAFPAHGQAMVKVNDNVN
                     FKLGFLIQPQADWTETINATGDGSNGFQQNLLIRRLRFLIGGQVAKNVFFFAETENSN
                     LGKSTQAVGSTTGTKSPGTGFNLLDAVGEWRIAKEFNVQFGELRSPVSREGLKSSPNQ
                     FMLDLSAYAFLTSSALQNNAGRDTGVMFRGWFLCDRLEYRSAIVSGLRQPGVKNSPRF
                     VNRLQYNFFDTEVYNLPSYAGVNYGNKKILAVGGAYDTQGAYRFASADMYLDFPVPVG
                     SFESTIQYQYANGGTFITTLPEQSTFQIEAGAYFKKAKIAPIARYEQKTFSQTINESK
                     NEYRYAFGLNYYPYAKSESNFNIKFWWQRVTVKCASSVSPCPASSSNFITDQVTLQMQ
                     AYYF"
     gene            30646..31860
                     /locus_tag="PLB01_00725"
     CDS             30646..31860
                     /locus_tag="PLB01_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HQQ75850.1"
                     /translation="MTPNRSSRLLAVLAAALLAVFPARSEDEIRLGAVLPVTGKESKI
                     GGAYKQATEFAVKEVNDAGGLDVGGKKMKVDLRLLDDTSEASKSAQLVEQLMAQQKVH
                     AVVGGYGSQLVQAQSVVPDRYEIPYVAGGAGATSIYGRSKWVFGTLSPVENLATTQME
                     FLSDLVKEGKLKPPLKIAIVWENTEHGKDFQKGIHDFVKAHPKEFSVALDESFEMYAP
                     DFKPLLTRVQAAKADLFMCDAHLEDYISMQRTYTQMGLYHLMITYGARGSDEAARKGL
                     GAATDWIFASGWWSDLLPYPQVKAWTEKYKAATGTTPQWYHACAYETVRAVFTAIKKA
                     GSLKPEAIRAALASLEIKDSILPGGVFKFSKTGQAVLPFVVTQNKANGKLDLVWPKDA
                     RTGPPVAPIPKG"
     gene            31874..32341
                     /locus_tag="PLB01_00730"
     CDS             31874..32341
                     /locus_tag="PLB01_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75851.1"
                     /translation="MPGGNRFAGVFDTAVGSTMRPGDPGTAKLTRDWGERLVSVRYRY
                     AADPPMRFTTIEIVVDAAPWKPSPARPVLVEVRSWESALRKRIVEAGGQWDARAGRWR
                     IRYDKVLALDLGARVQLTKKARGRTRRSKISTPEGTPTSLPKPKPPRNVGRGA"
     gene            32393..32875
                     /locus_tag="PLB01_00735"
     CDS             32393..32875
                     /locus_tag="PLB01_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75852.1"
                     /translation="MSEHHVFESALRDVAFAPNGAFRIRPTGADIVAAFVVTLFGASA
                     SLLGGVFALVFLANGWSFPLVEAQGMAFLVVAPVLFVLSAAPLVLHRARLLGAGQGVR
                     NLVFGVLLSVFSFPLLIVATNASPSLPAWLRVAGVLSAGFIGGLAAHGVSLLHRRAAA
                     "
     gene            32958..33407
                     /locus_tag="PLB01_00740"
     CDS             32958..33407
                     /locus_tag="PLB01_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75853.1"
                     /translation="MESTRLERLRAAAEAFRRALLAVPGLDLPVTLRTFPVGACGDTC
                     LLLGAYLHDLGLGPFAYVNGSRPVERAPGLLQSHAWLASTDGTVIDITAHQFSEFPEP
                     VYVGAATAWHGTFDSAQQGTADFRNYDPRTVEALGRVYAAIRSRVGV"
     gene            33994..34380
                     /locus_tag="PLB01_00745"
     CDS             33994..34380
                     /locus_tag="PLB01_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75854.1"
                     /translation="MRTLTLALALVFAAPRPLAADQTCVRTIGGRHIPAFVGLGSEGL
                     DFQKRCLVWEYSAEPKAEFIPSPETPSLGSHAQVVVIEPAPDRSSGSITLSELLSHSL
                     SLKPVASEAALSLMPAVSDAHPLTAK"
     gene            34401..34790
                     /locus_tag="PLB01_00750"
     CDS             34401..34790
                     /locus_tag="PLB01_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ75855.1"
                     /translation="MSRARISEIAPFFIVKDVAAALSFYRDALGFEVTFQEPAQDPFF
                     AIVARDGAMIMVKAVGVAPLPNCQRQRDARWDAYLAVPDPDALAAEFATRSVTFSVPL
                     EDTHDGLRGFELKDADGYVLFFGRPRI"
     gene            34860..35246
                     /locus_tag="PLB01_00755"
     CDS             34860..35246
                     /locus_tag="PLB01_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75856.1"
                     /translation="MANSKQIAGLIGPALVAMLVSEFPLVQPHLYDSQIPPVVYLSGV
                     LMFVGGLAVVRQHNAWARNWTVLVTLTGWFFLLLGLVRMFAASQYHQAASSTSSTVFM
                     ALEGILFVVAVILTYQAYRREPRKEP"
     gene            35251..35670
                     /locus_tag="PLB01_00760"
     CDS             35251..35670
                     /locus_tag="PLB01_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQQ75857.1"
                     /translation="MKKPGASREPSASDLISKRIAELGDWRGKTLGRMRRLIREADPD
                     VVEEWKWMGTPVWSHDGIICTGESYKSVVKLTFHKGASLKDPARLFNSSLDGNVRRAI
                     DIHEGDDVDAGAFKALVRQAVALNSSRPSKPPKKRKT"
     gene            35667..36329
                     /locus_tag="PLB01_00765"
     CDS             35667..36329
                     /locus_tag="PLB01_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75858.1"
                     /translation="MNLAVAFLALAAALPSPADGGVPVEQEPEHRTVFKNDYVQVFRV
                     TLAPGKATGMHVHAHDDAAVRLSAATVTSDSPGMPAGAPEPRAAGFVSARNNEPRALT
                     HRVRNVGTTLFDVLDVQILKRPAGPAAPPSSAPAAENPQMRVYRYELAPGAGSAQHVH
                     SRPYLLVAATDMDLRMTAPDGASMAHPVKAGDLHWVESAVTHTFVNEGKASGVLVEFE
                     LK"
     gene            36402..37097
                     /locus_tag="PLB01_00770"
     CDS             36402..37097
                     /locus_tag="PLB01_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HQQ75859.1"
                     /translation="MTSIRGKARMAGFLYLLLMAAPLRLLYIPKTLFVKGDAAATAAN
                     IAAHETLFRFGMVGDLFCGVVLIFLVLAFYRLFEAVDRTLAVLVVVLGGLLPATIDFV
                     NVVNDAAALILVRGADFLSVFDKPQRDALAMLFLRLHHQQVVAAETLWGLWLFPLALL
                     VLKSRFLPRFLGYWLILNGFAYLAESLTGLLLPQYEATVSNVLFPALLGEVALMLWLL
                     IRGADAGAAYSGT"
     gene            37119..37700
                     /locus_tag="PLB01_00775"
     CDS             37119..37700
                     /locus_tag="PLB01_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HQQ75860.1"
                     /translation="MGTGRLEAFSDGVLAIIITIMVLEMKVPHGASFETLSPLLPVFL
                     SYVLSFVYVGIYWNNHHHMLHTCTRVTGGVLWANLHLLFWLSLFPFATGWMGENHFAS
                     IPSALYGAVLLAAAIAYWVLQQVIIASQGEGSALKRAVGGDWKGKVSPALYVCAIAST
                     FFVPWLAQALYVAVAVLWLVPDRRIERSLARAD"
     gene            37760..38098
                     /locus_tag="PLB01_00780"
     CDS             37760..38098
                     /locus_tag="PLB01_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75861.1"
                     /translation="MPTPKTSLRFPLPPALRTRTLKLLTKLDGDEDPTRHTDDLCALV
                     LTLTESGMAHYFLKAVGDAKLGFVARQTASLGVSGAVRVMAPIVRSVLGGADAAQLRA
                     VSRHIHGLMA"
     gene            38127..38654
                     /locus_tag="PLB01_00785"
     CDS             38127..38654
                     /locus_tag="PLB01_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75862.1"
                     /translation="MTESRRLLRHFLAALAYRTQKALRGAPAGFGEFRAGVNVRTPHE
                     LVWHMTGVVGYARTMLHGGDFAPPRLATFEDEVARFHATLEALRDDLSDPLLEARISD
                     EQFLQGPLSDAMTHAGQLAMLRRLAGEPVPSENFIFAKVSADNVGPDQPEPAAPDAWW
                     RADQPPQPPGPPKKE"
     gene            38665..39015
                     /locus_tag="PLB01_00790"
     CDS             38665..39015
                     /locus_tag="PLB01_00790"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HQQ75863.1"
                     /translation="MTVFEKGFFTILGLSVVLILVAIPLALGKVPPNVVYGYRTRATL
                     ADEDVWYDANAYFGRRLIVASLCGVLAAWALYVLKPFPPDVFLPVSLVVLVAPTLVAA
                     VVTARYVRTLGQTR"
     gene            39066..40115
                     /locus_tag="PLB01_00795"
     CDS             39066..40115
                     /locus_tag="PLB01_00795"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQQ75864.1"
                     /translation="MSSNVRAYGAPAPNAPLVPLTIDRREPGATDVEIEILFCGVCHS
                     DIHQVRDEWWPGTFPMVPGHEIVGRVSRTGPGVTKFKAGDLAGVGCFVDSCLTCDPCN
                     RNLEQFCLEGAAFTYNSTEMDRKTPTQGGYSQRVVVKERFAVKVPAGLDLAAAAPLLC
                     AGITTFSPLRQWNCKPGDRVGVVGLGGLGHMAVKLAAAMGAEVTMLSTSRSKEADAKR
                     LGAHAFALTSEKGTFKKLAGRFDLIIDTISVPHDLDAHLGLLRTEGAMVVVGVPPVPA
                     SLKAMSVIMGNKKLAGSMIGGIAETQEMLDFCAAKKITADVEVIPAAKINEAYDRMLK
                     SDVRYRFVIDSASLA"
     gene            40131..41030
                     /locus_tag="PLB01_00800"
     CDS             40131..41030
                     /locus_tag="PLB01_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQQ75865.1"
                     /translation="MQDIAGVLVNTVISAALYATMAYGLAVIYGVMRIINLAHAGLMM
                     LGAYATFALFTRFGLDPFLSLLVVGPLFFLIGVVLYRLVVRRLPRSGATPSMESLLLL
                     FGVWLVLQNLAYVIWTGDTQSILTSYTMKSVPVLGVRVGVPSLFVFAGSVLSLVFLNV
                     VLKATYLGKAIRAVTQNRDAALLAGVDADRISAMAFGLGTAFAGVAGGMLSSLYAFTP
                     DFGRSFLLKAFCIIVLGGMESVTGVAAGALVLAFLENALGSWTTLPTSLQDAASFTLL
                     FVTLVAFPQGLPGLATRVRTAFK"
     gene            41027..>41979
                     /locus_tag="PLB01_00805"
     CDS             41027..>41979
                     /locus_tag="PLB01_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQQ75866.1"
                     /translation="MKRLPLAAGAALAAWTLAAPLFLERGTLNDVWNLAFAVILASAW
                     NILGGFAGQVSLGYSAFLGIGAYTTVLLSLKGVNPFWTLPLGAALAALFSVVIGLPAF
                     RLRGPYFTIATIGVGEAVRVVASSVSFTGGSSGLRMPAGSFDFTRNYEAMAALAIVTV
                     VLAALVKRSAFGHALAAIKQDIDAAESLGVDSTRFKLVAHALSAALVALAGSLYAINF
                     QYIAPGSVFDFRLSLAIVLAPIVGGVGTVAGPVLGAIVFSTLQIKLLSVPALRDSYLF
                     IYGGLLILVMLYEPKGLVGLARRLVARVRQAPAPPPEAARAG"
BASE COUNT         6205 a        15073 c        13933 g         6768 t
ORIGIN      
        1 atgctcgccg ccgcgggccc cgagagcctg acgtggctcg gccatgcgtg ctttctcgtc
       61 cggacgggcg ggttctcgat cctgacggac cccttcctgt ccgactacgc gagccccgtc
      121 tccggcctcg gcccgaagcg ctacgtgccg ccggggctcg cgaccgaaga cttgcccgac
      181 atcgacgtcg tccttctgtc gcataaccac tacgaccacc tcgacggaac gtccgtagag
      241 aagatttctt tgaaggtaag aggggaaagg gttgccgtcg tgccgacggg gctcggcccg
      301 ttcttcgcgt cgcgccgcta ctcggatgtc cgcgagcttg gatggggcga gggcaccctt
      361 ctaagaaagt cttcctcttc ggtctccctt tcctgcctcc cctccattca cttctccggc
      421 cgcacgccgt tcgaccggaa cgagacgctg tggtgctcgt ggaagatcgt ttcgccgacg
      481 ctctctctct acttcgcggg cgataccggc tacggccccg tgttcgccga tctgggccgc
      541 cagcacggcc cgttcgacgt cgcgctcctt ccgatcggcg cctacgagcc cgcgtcgatc
      601 atgcggcccg tgcacctgaa cccggaggaa gccgtcgccg cgggccgcga cctcggcgcc
      661 tcgaaactcg tcgcgatgca ctggggcacg atcgtcctca ccgacgagcc gccgttcgag
      721 cctccggcgc gtttccggtc cgcggcgcgc gccgcgggct acccggacga acgcgcgtgg
      781 gtcatgcgga tcggagagac ccgagaaatc cgctgacgag agcgaccgcc tcgtccaggt
      841 tctgcttctc cgtgcgcccg gaggaggcgc gcggcttgaa ggagtggtcg ccgtccttga
      901 ggaaggagat tcggatcttc tttgaaaggg gatagcgggc gatctcttcg cgtgttccga
      961 aagcatctct ctcgccctgg aggatcagcg ccggggtcat cagctgcttc aggtgggcga
     1021 cgcgcagctt gtccggctgc cccggcgggt ggaaggggta gccgaggcac acgaggccgg
     1081 cgacgccggc ctcgtccgcg accatgctcg cgatgcggcc gcccatcgac ttgccgccga
     1141 tcgcgacggc cttcgcgccc ttcttccccg cggcttcgcg gatcgcctcg cgccaggtct
     1201 cttcgaggac cggccctcga tccggcggct tcctctttcc ctcgcgccgc gcgtgcatgt
     1261 acgggaattc gaatctcagg acgcggaacc cctccttcgc gattcccgcc gcgacggcgt
     1321 tcatgaagag cgagtccatc ggcgcccccg cgccgtgcgc gaggacgagg gtgagcggcg
     1381 cgcgcgccgg gccgtcggcg atgaagcggt ccacctacaa tccctccgtg ccgaacaagt
     1441 atctctcgta cgacgacgag cggggcccga aggtgcgggc catgttctcg cggctcgccg
     1501 cgcgctacga cctcctgaac gacgtgatga gcctcggcct ccaccgccgc tggaagcgcg
     1561 acgccgtgga tctcgcgctc gcggggcgca cggggcccgt ccgggtcctc gacctgtgct
     1621 gcggcaccgg cgacctcgcg ttcctcgcca gcgccctcgc cgggaccggc tcgcgcgtct
     1681 tcggcctcga cttcacgctc ccgatgctcg gcgtcgcccg tgagcggcga acggccgcgg
     1741 ccgcctcgcc cgccttcgcg cagggcgacg cgatgcggct gccgttcccc gccgcgagct
     1801 tcgacgccgt cacgatgggc tacggcctga gaaacgtcgc cgacccgctc gcgtgcctga
     1861 aggaggtgcg gcgcgtcctc gcacccggcg gccgcgtcgt ggtcctcgac ttcggaaagc
     1921 cctcgaaccc ggtggcgcgg tcgctctacg gcggattcct catgggcgcc atgccggtcg
     1981 tcggctggct cgcccacgga gatccggaca cgtaccggta cattcccgag tcgctcgagc
     2041 gctatcccgc gcagcgcggt gtccgggacc tcatggaacg ggcggggctc tcgtcggccc
     2101 gctacgagga ccgcatgttc gggacgatgg gcctcaacgt cggggaggcg ccgggccgct
     2161 cccccctata atcgcgcgtg ccccgcgaca cgcttcccgc gagtccggaa ccgcgctccg
     2221 ggcggcttcc gggcgactcc cccgcgcagg cgtcgcggct cgtggcgctc ctcgtcgtct
     2281 tcttcgtctc gatcatcgcc gcgattctcg cgacgggggc gatcgggatg tcggccctct
     2341 ccggggtccg cgccttcgtc gcggcggagt ccacctggtc caaggcgcag aaggaggccg
     2401 tctacgcgct ctcgcgctac ctggacaccg acggcgagga atcccactac cggaggttca
     2461 acgctctgct cgccgtgccg cggggcgacc gcaagggccg gatcgcgatg tcaggcccgg
     2521 cgcccgacgc ggcggccgcg ttccagggat tcctcgaggg gcgcaactcc ccttccaacg
     2581 cggaggagat ggtctcgatc ctggcgcgct tccgggacac ggagcacatc cagcgcgccg
     2641 tccagatctg ggagcgcgcg gacgcggtga tcgacgagct cgaggcgctc gcggtcgtcg
     2701 tccatcagga tttcgaggcg ggccgcatgt cggcggaggc gcgcgcggcc tacaaccaga
     2761 gggtcgaaaa gctcaacgag aaggccgctc tcctcgagga cggcttcacg gccgcgttcg
     2821 accaggcgtc gcgcgtgttc cggacgcgcc tctacgccct cgtcgccgcc atgacgctcc
     2881 ttctcgtcgg gctcggcgtg gccgcgtccg tcttcgtcgc acgcgtgctc aagcggcgca
     2941 actcggcggt caccgcctcc gagcggcggt accgcctcct gttcgaacgg aacctcgcgg
     3001 gtctctaccg cacgacgctg gacgggaaga tcctcgactg caactccgcc ttcgcgcgca
     3061 tcctcggcta cccgtcccgc gaggaggtcc tcaagatcac ggcgctcgac ctctactcgg
     3121 atcccggaga ccgggagtcc ttcgtcgcac gcctcaagca gcagcgcgtc ctcgtgaact
     3181 tcgagctctg tctcaggcgc cgggacggga gccccgtctg ggtcctcgcg aacgagagcc
     3241 tcatggacga ggccggaggc gacgtcgtca tggacggcag cctcatcgac atcacggacc
     3301 gcaagcgcgc cgaacagcag cgctggcacc aggcgaatca cgacgcgctc acggaccttc
     3361 cgaaccgggt cctgttcaac gaccggctct cgctggcgat cctgcacgcg cagcggcgga
     3421 ggcagtcgct cgtcgtcatg ttcctcgacc tcgaccactt caagcgcgtg aacgacacgc
     3481 tcggccactc cgccggcgac gagctgctcg tcaaggtggc cgaccggctg cgccgctgca
     3541 tccggcagga cgacaccgtc gcccgcgtcg gcggcgacga gttcctcctc ctcctgaacg
     3601 ggatcacgcg cgaggccgac gccgccacga tggcgcgcaa gatcctcggg atcctcgccg
     3661 agccgttcct cgtgcagaag cgcgagctct tcatcgaggc gtccatcggg atcgggctgt
     3721 acccgggcga cggcggcgac gccgagaccc tcatcacgaa cgtcgacacc gcgatgtacc
     3781 gcgcgaagga gacggggcgc aacagcttcc agttcttcac gcgcaagatg caggaacagt
     3841 cccaggagcg ggccgccctc gagtcgggcc tgcggcgggc gctcccgcgc ggagagttcg
     3901 tcctgcacta tcagcccatc taccgtctgc gcgaccgctc ggtcgtcggc gtcgaggcgc
     3961 tcgtccgctg gcggcacccc gagaaaggca tcctgtcgcc gcgcgacttc atcccgctcg
     4021 ccgaggacgt cggcctcatc gcgcgcgtcg gggaatgggt tctccggagg gcgtgcgagc
     4081 agctcagggc ctggcaggcg gccggggcgg ttccgatgcg catctccgtg aacgtctcgc
     4141 cgcgccagtt ccagcagcgc gatttcgcgg ccaacgtgcg ccggatcgtc cgggagtccg
     4201 ggctcgagcc ctcggtcctc gacctcgaga tcacggagtc catcgcgatg cacgacgtcg
     4261 cgcacacggg gcgggtcctt tccgagctcg cggatctcgg gatccagatc tcgatggacg
     4321 atttcgggac gggccactcg tcgctgaact acctgaagca cttcccgatc cgccgcctca
     4381 agatcgacca gtccttcgtc gccgggatga cgacggacga caaggaccac gcgatcgtcg
     4441 cgacggtcgt gtccatcgcg cggaacctcg gtctcgcggt gacggccgaa ggcgtcgaga
     4501 cggaagagca ggcggccctg ctggcggccc tcgcgtgcga ggacgtccaa ggctacctct
     4561 tcgcgcaccc cgagcctgcc gaggaagtcg agaaacgtct cgggctcgcc gcacccgcca
     4621 ccggcgcctg attcaaacga actatcgccg gaattgcgct ccggggagcg taatagcgcg
     4681 agtctggagg gcccgcgcat gcgcgtcgac aaatggatga ccccgaaggt gatcacgctg
     4741 aaggcgacgg attcgctctc cgcggcgatc cacctgatga aggagaagaa gatccgccgc
     4801 atccccgtcg tcaacggcga cgggaagctc gtcggcatcg tctcggaccg cgacctgaag
     4861 gacgtctccc cgtcccgcgc cacgacgctc gacatctggg agctccacta cgtcctcgac
     4921 aagctgaaga tcggcgacat catgacgaag aagccgttca cggtgacgcc cgacaccccg
     4981 atcgagcgcg ccgcgcagat catgatggag aagcgcgtgg agggccttcc ggtactcgac
     5041 gcgaagggcg cgatggtcgg gatcctgacg gagggcgacg tcttccgcgc cctcgtggac
     5101 atcacgggcg tcgggaaggg ccgcctccgc gtcgacctcc tgatcccgga caagcccgga
     5161 tccatccgcg aggtcgccga cctctgccgc acggcgggcg gaaaaatcgt ctccatcctc
     5221 gggtcctacg cgaaggtccc ggcgggcaag cgcgagctca tcatgcgcgt cgactgcccg
     5281 aaaccggacg tcctcagggc cgatctcgcc aaggcctacg gcgacgcccg cgtcgaagcc
     5341 gactgattcc cggggctggg cccgttcgcc ggggccccgc ctctctccgc gccggggccc
     5401 ccgccccggc gcatcttttt gctccgatct gattcattcg aatcatcgcg ttcgagaacg
     5461 aatctcgaca taacggtgcc gcttcggtat ccgaaaaacc aagaggaggc cttacatgac
     5521 catccggcgc atgtcctcag tgctcgccgt cgcggccttt gccgcgttct cggcgtcccc
     5581 ggccctcgcg cagggcaaga ccttcacggt cggcgtgcct ctccccctca ccggcgccga
     5641 ggcgaagttc ggcgaaatga agaagcaggc ctacctgatg gcggcggacg agatcaacgc
     5701 caagggtggc gtgaagggca ccaagctcgc cttcgacatc caggactccg gcggaaagcc
     5761 cgacaccgcc tcggcgatcg tcgagaagtt catcacgatc aacaagtacc cgatcgtcgt
     5821 cggcgagtac acgagccagt gctcgtacgc cgtcgcgggc atcgccgaga agtacaaggt
     5881 cccctacctg gtcgacaccg gcgccgcgga caagatcacg cagcagggct ggaagtacat
     5941 cttccgcctg aactcgcccg cgggcctcta cgccaagggc gtcttctcct tcttcgagga
     6001 agtcgccaag cccaagacgg tcgcgatcct cttcgagaac acggacttcg ggacctcgac
     6061 ctcgaagtcc atgaaggact acgccgagtc ccacggcatg caggtcgtcc tcgccgaggg
     6121 ctaccaggcc ggcgccgtgg acttcaagcc catcctccag aaggtcaagt ccctccgccc
     6181 cgacatcgtc tacatggtgt cgtacctcat ggacgcctcg ctcctcatgc ggcagtcgaa
     6241 ggagctcgac ctcaacgccc aggccttcat cggcggcgcg gcgggccaca cgctccccga
     6301 gttcctcgag aacgccggcg acgcgaccga gttctgcatg tcggcgtcgc tctggacgcc
     6361 ccaggtgaag tacccgggcg ccgccacgtt cgacgcgaac ttccagaagc tctacaagaa
     6421 ggcgcccagc tatcacgccg ccgaggccta cgcgacggcc ttcgtcctcg ccgacgtcct
     6481 cgcccgcacg aagtccatgt ccagcgacga cctgcgcgac gcgctggccg cgacggacat
     6541 gatgacggcc ttcggccccg tgaagttcca gtcctgggac aagttcacga accagaacaa
     6601 gatggagacg ctcgtcggcc agtggcagaa gaagaagttc gagacggtgt ggccgaaggc
     6661 tctcgcgtcg gctccctacg tctaccccgc gccgcgctgg cgcgaccgca agtagcgcgg
     6721 cggcgtcggc gggagtgatg tcgtgacgcc acaggtcgcc ggtcaggtcg agctgctcgg
     6781 gcaggtcctc atctcgggga tcctgctcgg cgggctctac gccctgatcg gtctcgggat
     6841 gtctctcatc atgggggtca tgaagatcat caacctcgcg cacggcgagc tgatgatggt
     6901 cggcatgtac atcaccttct gggcgttcac gctcggaaag atcgacccgt acgtctcgct
     6961 cctcatcgtc attcccgcgc tcttcgtcct cggggcgatg cttcagaaat ggctcatcgc
     7021 cccggtcatg gaggccgagt cgatcctgcc ggagaaccag gtgctcctca cggtgggcat
     7081 cggcctcgtg ctctcgaacc tcgcgctcct cgcgttcacc gccgactacc gctccgttcc
     7141 cgtctcgtac gcgtcgaaga cgatctactg ggacgtcaag atgggcgggc agaccgtctc
     7201 gctctcgttc agcttcgcga tgctcgtcgc gttcgggatc gcgctcgtcc tcggaggcgc
     7261 gctcttcctc ttcctgggct acacggacct cggccgaatg atccgcgcga cctcgcagga
     7321 cgcccgcgcc gcgtccctca tgggcgtcaa cacgaagcgg atcacgtaca tcacgttcgg
     7381 cctcggctcc gccctcgtgg gcgccgccgg agccctcctc gccccggtct actacctctt
     7441 cccccagatc ggcggcccct tcacgctgaa ggccttcatc atcacggtcc tcggcggcct
     7501 cggaaacgtc gcgggcgccg tcctcggcgg cgtcgtcctc ggcctcgccg aatcgctcgg
     7561 ctccgtctac gtctcgctcg gctacaagga cgcgttcggc ttcatcatct tcgtcctcgt
     7621 cctgatcttc ctgcccaagg gcctcctcgg gaaaggcagg agctgacgat gagccgctcc
     7681 gtcctcctcc ctctcgccgg cctcgcgctc ctcgcggtcg tccccgccgt cgtgagcaac
     7741 ccgtacgtcc tccacatgct gatcctctgc ctgctctgga cggtcctcgg gcagagctgg
     7801 aacctcctcg ggggatacac gggacaggtc tccttcgggc acgccgcctt cttcgggatc
     7861 ggcgcgtaca cgaccgggat cctcgtgaag acggggatgt ccccggccgc cgcctggtgg
     7921 ggcctcctcc tcggcggcct cgcggccgcg gccgcctccg ccgtgatcgg ctggatctgc
     7981 ttccggctgc gcggcccgta cttcgcgctc tcggtgctcg cgctcgcgga ggtcctccgc
     8041 ctcgtcgccc tgaactggaa gcagctcacg aacggtgccg agggaatcct cttcatcccg
     8101 gccttcaact cgaagagctg gtactactgg atcgtcctcg cgctcgccgc cgcgagcatg
     8161 gcgctcatcc ggttcgtcat gcacgggaag ctcggcttct acttcctcgc gatccgagag
     8221 gaccaggact gcgccgaatc gctcgggatc aacgcgacga agtacaagct gatctcgctc
     8281 ctcctgagcg cgttcttcac gggcgtcgcg ggcagcttct acatgaacta catggggttc
     8341 atcgacccgg gcatcgtgtt ctcgatccac gacatctcgg tcatgatgat cctcgtcacg
     8401 atgctcggag gcgtcgcgac gacgtgggga cccgcggtgg gcgccgcgat ctacatcctc
     8461 atgtccgagg cgttccgcac gtacctgaag tccgggcacc tcgtcttctt cggcatcctc
     8521 atcatcgtga tcatcatctt cttcccccag ggcatcgtcg gaacgctccg ggaaaccctc
     8581 cagcgccgga aacggcgcct cgcggccccg ccggccggcg gcccgctgaa ggaggccgca
     8641 tgagcctcct cgagctgaag gacgtcacga agcgcttcgg cggcctccag gccgtcggcc
     8701 acatgacctt cacggtcgag aagggcgaga tcttcgggct catcggcccg aacggcgccg
     8761 gcaagacgac cgtcttcaac gtcgtgaacg gcttctacgc gccgaccgag ggcgagatcc
     8821 tcttcaaagg cgagcgcatc gacggcttga agccctacga gatctgccgg cgcggcctcg
     8881 cgcggacgtt ccaggtcgtc aagcccctcg cgcgcctgac cgtcctcgag aacgtcatgg
     8941 tctcggcgtt cgcgcgcacg gaccaccggg acgaggcccg cgcgatggcg ctcgaggcca
     9001 ccgcgttcac ggagatggaa gcctggcggg accgcacggc gggaagcctg ccgctcggga
     9061 tgcgcaagcg cctcgagatg gcccgcgccc tcgcgacgaa gccggagctc ctcctcctcg
     9121 acgagaattt cgcgggcctc aaccccgccg aggtcgagaa gaccatcgcg atcgtccgca
     9181 agatccacga gagcggcgtc acgatcctca tcatcgagca caacatgcgc gtcatcatgt
     9241 ccatctcgca gcgcctcctc tgcatgtgct acggcgaacg gatcgcccag ggcacgccgc
     9301 aggaagtcgc gaacgacccg agagtgatcg aggcgtacct gggagccgcg catgcttgag
     9361 gtcaagaacg tcgacgtgtt ctacggggac gtccaggtcc tctgggacgt gtccttcaac
     9421 gtccagaagg gcgagatcgt cgccttgatt ggcgcgaacg gctcgggcaa gacgacgagc
     9481 ctgaacacga tctcgtcgat cctcaagccg aagaagggcg ccgtgacgta cgaggggcag
     9541 gccctccaca cgcgcgctcc ctacgacctc gtcgggctcg gaatcgccca cgtcccggag
     9601 gctcggcggc tcttccccga gatgaccgtc aaggagaacc tcctcctcgg cgcgctgacg
     9661 cccgaggcga agaagacgcg ccccgagatg ctcgagaagg tctacgggat cttcccgcgc
     9721 ctcaaggagc gggagaagca ggcggcgggg acgatgtcgg gcggcgagca gcagatgtgc
     9781 gcgatcgccc gcggcctcat gtcgaagccg cgcctcctca tgctcgacga gccgtccctc
     9841 ggcctctccc ccatcctcgt ctcggacatc ttccgcgtca tccaggaggt ccacgacgcc
     9901 ggcgtcacgg tgctgctcgt ggagcagaac gtcttcaaga gcctcgcaat cgcggaccgc
     9961 gcctacgtgc tcgagaacgg gcgcatcgtc ctcgagggca agggaaaaga cctcctgaac
    10021 gacgcgcacg tcaaggaggc gtacctgggc gtctgacccc ggccagcgcg accttcacgc
    10081 gctccggcgt catgggcatc tcggtgaggc gcgcgcccgt cgcgtcgaaa acggcgttcg
    10141 cgatggccgc cccgaccggg acgagcgccg gctcgccgcc tccctgcggc gagagctcgt
    10201 cgttcttcac gagcaccgtg tcgagcgccg gcatcatcga gaacttcggg atctcgtacg
    10261 tgtcgaagtt cttcgtgagg acgcgcccgc cgtcgaagcg gagctcctcc gtgagcgcgt
    10321 agccgagacc catcatgatg cagccttcca tctgcatcgt ggcgccgtcc gggttgatca
    10381 cgacgcccat gtcctgggcg caggcgatcc gcacgacgcg gatccgcccg gtggcggcgt
    10441 cgaccttcac ctcggctgcg agggccacgt acgtcccggc gtcgatcccg caggcgatgc
    10501 cgacgccgcg gcccgacggc ccggcggcgt tcttccagcc gaagcgcgcg gcgacggcct
    10561 cgagcacgct ccgcatgcgc ttgtcggacg tgttccggag gcggaactcg agcgggtcgg
    10621 ccttcgcctt cgccgccatg acgtcgaggt gtgactcgcg cgcgaacacg ttcaggttcg
    10681 cccccggggc gcgccacggc cccgtcgcga agaggtgcat cttcgggctg tcgctccccc
    10741 agcgtccgta ggtgcgcatg aggttgtgcg ggacgtcgta gaactgctcg gcgcctcgcg
    10801 agcccgccgc gtagacctgg tagtcccaga gcgagatccg cccggcgccg tcgatcccgg
    10861 accggatctt cacgacggcc gccgggcgga acgcgtcgaa gaagaactcc tcggcgcggc
    10921 tccagcacac ctggaccggc ttccccgtga tcttcgcgag ctttgcggcc tcgatggcct
    10981 gcaatccgcc gccgctcttg ccgccgaagc cgccgccgac gaacggcgtg atgacgcgga
    11041 cgttctcctt cgcgaacccg atcgcctgcg cgatcgcggc ctggttcggg aacggcgtct
    11101 gcgtcgagtt ccacaccgtc atcgttccgt tctcgagccg cgcgagcgcc gtgtgcgtct
    11161 ccatcggcgc gtgcgcgccg tagccgttca cgtactgccc ctcgaagagc gaggccgagg
    11221 ccttctcgcc ttccgcgagg cttcccttct tctcccgctc ctcgcccgct cccgcgaccg
    11281 cgaggaggtg ggcgaagacg gtccggtcgt cgaactccgg cttcgggacg tcccagtcgg
    11341 acttcacggc cgcgagcgcc ttctcggccg tctccgggtc gggcgcgaga acggccacga
    11401 gcccgccctc gttcacgacg acggcgcccg ggaaccgcgc ggcggccgac gtgtcgacgc
    11461 tcttcatcac ggcgccgtgg gccggcggac gcaggacctt cgcgtacagc attccgggca
    11521 ggcggatgtc ccccgcgtag gcggccttgc cggtcacctt ggcgcgcgcg tcgaggcgct
    11581 tcgtgggctt gcccatcagc cggaactcgg cgacggactt ctggacggcc ttcccctcga
    11641 gcgtcctgac gatcttctgg ccgttcgcga gctggccgaa cgtgacgcgc accttcgggt
    11701 ccgcggtcga gaccacggcg ccgtcctcga cgcggagggc ttccctgggc acctggagct
    11761 tctcggaggc gagcgcgacg agcaccgcct tcgcctccgc cgccgccgcg cgcagcgccg
    11821 ggccgaagaa gcgggtcgac atcgagccgt acgttccgtt gtcgaacggg cagagcgccg
    11881 tgtcgcccat gaccatgtcg acggaggcga gcggcacccc gagctcgtcg gccgccatct
    11941 gggcgagcga cgtgacgacg ccctgcccca tctcgatctt cccggagtag acggtgacgc
    12001 ggccgtcggc cgcgatccgc aggtaggcgt tgaagtccgt cgggtagccg cgcgtgcgcg
    12061 tttcctgccc cacgaggtcg gacaggtcgg cgttgaagag gacgatgatc ccgccgccga
    12121 cgagacggac gaagcgacgc cggctgaagg aagcctccga cggggtcggc ggcagaacga
    12181 ggggaggccg ggcgccgctc acgacacgcc tcccgcggcc gccttctcga tcgcctggac
    12241 gatccggtgg tgcgccgcgc agcggcagag gttgtcctcc atccccgcga cgatctgggg
    12301 ccgcgagggc ttcgggttct tcaggagcag cgcgtaggcg ttcatgatca tcccgggcgt
    12361 gcagaaaccg cactgcaggc cgcccgtgtc cgcgaacgcc tcctggagcg gatggagctt
    12421 tccgttcttc tcgaggccct cgatcgtcgt gacgtccttg ccgcggacgg ccttcacggg
    12481 caggaggcac gtccgcacgg cctccttgcc gatgacgacc gtgcaggcgc cgcagaggct
    12541 ctcgccgcag ccgaacttcg tccccgtgag gccgagatcg gtgcgaagga cccagagcag
    12601 cgagcgctcg gggtcgacgt cgagctggac gggttttccg ttgaggcgga acgagacgga
    12661 gtccttcatt caagacctcc cgaagctcgg gtcgatcata gaaccgcccc gcgcggggcg
    12721 ggcgccgctc agcgaggcag gagcgtgcgc gggtcgttcg tgaccgcgtc gatcacggaa
    12781 ccgtaggcgg cgaacgagcc gccgggcgtg ggcgtcgaga ggacgagccg agcccccgac
    12841 acgtccgccg cgacgccgag gtcacggacg acgcgcgtca cctgcgtcat cccgagcggc
    12901 ggaagcgcat agcgcttcgt ccccagcgac gcacccgttt cggccacgag cgcgacgtct
    12961 acgtcgaggg gcgtctcggt cgcgttcgcg aggatgaggt tcgtgcggaa agccgcgtct
    13021 tcgcgcacgc cgaggatgga gcgcggggcg gcgcccacga gatccgccgc gccgaccgcg
    13081 ggaacgctct gcccgaacgt cccgccgccc ggcgagggcg tcgacgtctg ccccgccacg
    13141 gcgagcgcgg cggacgacga ggcgacccgg atcgcgccgt agcccgacga cacgccgaag
    13201 acggagccga ggacgtcggc gtacgtgacg gagcgccccg cggcgaggga gaacgactgc
    13261 tcggcgccgt tccggccgtc gacgtcgtga tcgaggaact tcagccccag ggaggcgtcc
    13321 gcggcgcccg tgttcgcgat cgtgaggtcc gtcgtgtaga acgcgccgcc ctgacccgcg
    13381 gcccgggcgc tcgacggcag gatccacgtc gccgcgggcg gaggggcgga ccctccggag
    13441 atcgtgtagg agaaggcgac ctgcccgctc ttctgggtcg cgctctcgtc cttcggcagg
    13501 aattcccgga tgtagtccac cttcacggac gacggcgaga cggtgacctt cacgtagccc
    13561 gagttcggca ggatgtcgcc ggacttgtag gcgtcggcgt tgaacgcggt gtaggtgtag
    13621 tccgcggggt tcgggagctc ctggtacgtg acgccgtcgg cctcctggcg ggcgaagagg
    13681 tggtcgtggc cctggaagaa gatcgtgacg tggttctggg ccatcagctg gtggatcggc
    13741 aggggccacg tcggacgctt gaccgcgaaa ccgtatgagc cgtcggcgct cttccctccc
    13801 cactcgaact cgtcggcgat gtcgacgccg cctcggcccg tccccatgac gtggtgcgcg
    13861 aagacgaact tccacttcgc cgtgctgctc tcgagggtct gcttgagcca cgcgtactgc
    13921 gcatcgccgt gcgagatgag ccagcggtct gccgtcttgc cgctgttgct cccgatcccg
    13981 ttgtccacca tcgccggaga cgtccagtac gggtcgatca cgacgaacag ggcgtcgccg
    14041 ttctgccagg cgtagtagtc gcgcagcggg ccggagatgc cggccagcgt catcgtgttg
    14101 ccggagtaga acgtgccggt cgtcgggtcg ttcggcgcgg gcatcgggta gtagaggttg
    14161 cgagcgttct gggcccagat cgggacctgg ttactgttcg tgccgtccgg cggcaggttg
    14221 tagttccaga gcgaggtctg ctcgtggttg ccggtcacga ggaacacggg agccgagcgc
    14281 ccgacgatct ggaggtacgg gatctgcagc gtgtagcgcc ccgtgaccgc gctctgcgtc
    14341 cagggcgtcg gcagcgtgtc gacgctgaag tcgtcgccgc tcgtgaggaa gaagtccggt
    14401 cgttccgcgg cgaccgccgt caatgtctgg atgtagagcg ccgggtcgaa catcgacttc
    14461 tcgcgctcgg gatgcgagtc gccctgcgcg cagaagacga acgagctgcc cggagcgcgc
    14521 tgcgtctgga acgaggactc cgtcccgggc gtgaaggcgc ccgcggagcc ggccgcgcgc
    14581 gtctggacgc ggtagtagta ccgcgtattc ggctgaaggc ccgaaaggac cgtctgggag
    14641 acgtagaagc cgccgacgta gggatctgcg cccatcgcca cggacggggt cgtcgcggtg
    14701 tagacgcccg cggcgaggcc gtactggacg ttcatttcga gggcggcgtc ggcgcgggtg
    14761 ttcagcgtga tggaccggtc cgtcggctgc cccaggagct gggtgaccgt cgcggcgccc
    14821 agcggccgcg cggcgaggag agcgaggaaa acgaggactc tcttcatcgc gggaggagcg
    14881 tcctcgggtc gttcgtcgtc gcgtcaatca cggaggcgta ggccgcgaag gcgccgttcg
    14941 tggtcggcgt cgagaggacg aggcgcgcgc ccgccgtctc ggccgaaact ccgaggtcgc
    15001 gcacgacccg cgtcacctgc gtcatgccga gcggtgggag cgtatagcgc ttcgaggcga
    15061 gcgcggcccc cgcgctcgag acgagggcga cgtccacgtc gaccggggcc tccgcggcgt
    15121 tcgcgaggat gaggttcgtc cggaacgcgg cgtcctctcg gacggccgcg atcgaccgcg
    15181 ggatccccgc tttcacgagg tcggcgtcgc cgaagcccgg aacgctctga ccaaacgtcc
    15241 ctccggagcc cgcgggcgtc cacgtctggc tcgataccgc gaaggccgtc gtcgacgccg
    15301 tcacgcgaag ggcgccgtag cccgtcccca cgccgaagac cgagccgagg acgtccgcgt
    15361 aggtgacgcc ctttccggcg gcgagggtga acgtcttctc gtctccggcg gtgccgtccg
    15421 cgtcgtgccc gaggaacttc acggcgtacg tcgcgtcgac cgcgcccgtg ttcgcgagcg
    15481 cgagatcggt cgaatagaac gcgccgccct ggcccggggc gcgggcgctg gacggcagaa
    15541 tccacgtcgc ggcgggcggg ggcgccacgg acttccccgc gacggaatac gaatgaacga
    15601 cgtcgccgtt gtggcgcgtc gccgtctcgt cagcggggag cagcgaccga acgtagtcca
    15661 cggtcgcctt cccctcggcg atccgcaccc gcaggtggcc agagctgccg tagagcgtgc
    15721 ccgtgacgta gccgtattcg accgcgctgg aagtcgagtc ggcgcgcggg aagcccggct
    15781 gcgggacctc ctggtagacg atcccgccga ggtcctgctt cacgtacagg tggtcgtggc
    15841 cgtggaagac gaccgagacg ccggtgcgga cgaagagcgc ttggagcggc tcggcccagc
    15901 ccgggcggtg cgccgcgaag cccggcgtgc cgtcggcgtt cgcgcccccc cactcccaga
    15961 ggcccgcggc ctccgccccg ccgcggcggt ccttgccgaa gccgccgacc aggtggtgga
    16021 tgaagacgaa cttgagcttc gcggagctcg actccagcgt gcgcttcagc caggcgtact
    16081 gctcggcgcc cagggtccag ttccagttgt cggagccgtt gcccttctcg cgcgtgggcc
    16141 agaaggggtc gaggacgacg atctgggcgt cgccccacgc gaaggagtag tagttccgga
    16201 gcagcccgac cgccggctcg ggcgtcgcgt tgcccgtgta gaacgcgtcc ggggaggggt
    16261 tcgggaagag cgcgaggcgc agccgggccg accaggcggc gatgctgttc gccgtgccgt
    16321 cggcggcgta accgatttcg ccgtcgtggt tgccgagcgt gaagaagacc ggagccgacg
    16381 cgccgatcag gccgaggtag taccgctggg cgtagtactg gggctgagcg tccgtgaacg
    16441 cggcgccgta cttgtcggtc atgaacgtgt cgccgaggtc cacgtggaaa tccggcgcgt
    16501 ctgcggccgc gttctgcatc gtccgggcga agagggccgg atccgtgttg ttgtcgagat
    16561 gcgaatcggc gtggagcgtg aacgtgaacg cgcttccggc gggccgcgcc gtgcggaacg
    16621 tccgggaggg agtcgccgtg tacgcgccgg acgtcccggc gcgccagagc aggcgatacg
    16681 aatacgcggt gtccggctgc aggccggcga cggggaactc gacgccctgt tgcgcggcga
    16741 gggccttcgt cccggtcgcg gccggcgccc cgcccgccgc gggaacgacc tcgacgtagc
    16801 cgtcgccgtc ctggtaagcg accacgctca cgacgatcga cgtgccggtc ggctgcccga
    16861 ggatgaggtc caccgggtgc gccgggacgc cgaagtcggc agccagcggc agcccgacga
    16921 gggcggccat ggccgcgagc gcgagacgag acgacgggcc cttcatcgcg gcaggagagc
    16981 cctcgggtcg ttcgtcgtgg cgtcaaggac ggaggcgtag gccgcgaggg cgccgcccgc
    17041 cgtcggggtc gaaaggacga gccttgcgcc ggcgacgtcc gcggccacac cgaggtcgcg
    17101 cacgatgcgc gtcacctgcg tcatgcccag aggatagagg gtgtaccgct tcgaggcgag
    17161 gctcgcgccc gcggccgaga cgagcaccac gtcgacgtcg agcgttgcct ccgtggcgtt
    17221 cgcaaggacg aggttcgtgc gaaaggcggc gtcctcgcgg acgggcgcga tcgacctcgt
    17281 aacgcccggc ccaacgaggt ccgcggagcc gcaggcgggc acgctctggc cgaacgtccc
    17341 cccggccggc gcgggcgtcg aggtctggct cgagacggcg agcgatgcga cggtcgaggc
    17401 gacgcgtacg ccgccccacc ccgagctcac gccgaagacg gagccgagga cgtccgcgta
    17461 cgtgacggtt ttccccgcgg cgagggcgaa ggtcctctcc gtgcccgagc gcccgtcggc
    17521 gtcgtgcccg aggaacttga gggcgagcgt cgcatccacg ccgcccgcgt tcacgatcgt
    17581 caggtccgtc gtgtagaagg cgcctcccgt cccggcggcg tgcgcggcgg acgggaggat
    17641 ccacgccgcc acgtaggccg gagcggccgc cgcgaagaag cggaccgagt cggcgacgta
    17701 aggcgcgagc gtaacggagt cggcgggcgc ggcgtaaagg tagtacgtgg cgcacgtcgt
    17761 gaagtcgaac ggcgtgccgt ccgcatatcg tccggacatg aacgagcgcc ggagcccgac
    17821 gagccggtcc ttctgcgctt gcgtgagcgt cgccgcgacg cgcgcgaacg ccgccgcgta
    17881 ggcggccacg ttcgccccgt ccagctcccc gtagattccc gactgctcga ggaccgtggc
    17941 cttgacggcc gcgagctgcc ccgccgtggg ttcagcggac gagatgaggc ttcggagggc
    18001 ggtcgagatg gccgtccgcg ccgagacgat gctcggcgtg ccggcgtaga gcgcgctccg
    18061 ctgagtgtcc gcgagcgcgg acacgatcgc ggcctgctcc gccgtgacat aacccttcga
    18121 cgcatcgcag agagccgatc cggccgtgac ggtcagctgc tcgtcgatgg agtagccctc
    18181 gtgtccgatc gcgggagcgt ctttcatgta gaaggatccg aagtaggtcc cgtgccgctc
    18241 ggggcagaag tagacgtcgc ccgcgagcga accgacgtac cagctgaaaa ggtcgcccgc
    18301 gtaggtcatc acggcgacaa cctcgtcgtt cgtgaggccc tgcgtcgtgc cgcggacgtc
    18361 gtccatcgtg cgcggcggcc aggacgccca tcccttgccc gccatcgccg agaggtaggc
    18421 cttctgggac gccgtgaacg agcggtagat ccgcgcgtac acgacggcac ggtcgtagct
    18481 gagctggccg tcgagcacgt agagctcgcg ggaggcggcc ttgacggccg cgacgtcgag
    18541 gcccgtggtg cccgccggga ggttcccgtc cacgaggcgt cggaaagcct gcatgagcgg
    18601 gtagcggcca tacgcgtacc ggttgatcgg ctccacctgc gtcgacgcga gcgccttgag
    18661 ccagccgagc tgctcggccg tgaggatcga gaggacgtcg cacgcggcac gcgtgaggaa
    18721 gctcgtgttg tgccccatgt cgtcggggtc gttgtcgcgc aggtactgga agccccagta
    18781 gtccgcgacc ttcccgggcg ggaagaacga ctgggcctgg aggtttcccg tgaccatccc
    18841 gaggccgtcg aaggcgagcg tggtccgctg cgcgccgtcc gtgagcgcct gggcgaggtt
    18901 gaaggcgggc ggggacgcgg gagtgatcgg aatcgaaagc gtcccggtcg tcgaggctcc
    18961 gccggcgtcc gtcacgcggc aggtcagggc gacggcgccc gagacgcccg ccgagaacgc
    19021 gacgctctcc gtcgcggcgc cgaccatggc ggcgccgttc gagacggtcc acgcgtagct
    19081 cgcgcccgcc tgcgcgggca ccgaggccgc ataaccggcc tgtccggcgg tgacggagga
    19141 cggggcggtg atcgtcgcga cgggcgcgcc ggcggcggcc cccgcgagaa gcgcgacgag
    19201 cagggcggct ctcatttcga cttcctttcg ccctccgcag acggcggggg cgcgtcctcg
    19261 cgggcggggc gcggggggcg gtactcgtcc ggcgtgagac gaccgtcgcc gttcttgtcc
    19321 agcttcttca acgccgcgga tgcgtttgcg atctcgtcgg cgtcgatgac gccgtcgccg
    19381 ttcgcgtcga gggcgacttc cagagggggc ttcggtcgat gcggcgggcc gccggccttt
    19441 tgcgcgggcg cgtgccccgc cacgaggaac gagccggccg ccacgaccag ggcgagcgcg
    19501 ggcagcgcga cgcggccggg gcggagcggg agggacggtg tgcgtgggct catcttggtc
    19561 aacctccacc ccaaacgtag acgcgcaccg gtcgcgccgc ttcacgcctc tgcaagttgt
    19621 tcgtaagaga tgtaaggcct agccgtcgaa gcggtagccc agccggtgga ccgtcacgac
    19681 gtagaccggg tgcgacgggt tcggctcgat cttggcgcgg agcgaggcga cgtggacgtc
    19741 caccgtgcgg gacacgggtg tcgcctcgta gccccagacc tcgtcgagca ggcggtcgcg
    19801 cgagaggatc tcgccgcggt gctcgatgaa gaaccgcagg agccggagct ccatgctcga
    19861 gagccccagg acctcgccgg cgcgggtgac ttcggcgcgg cggaagtccg cgcgcacgtc
    19921 gccgaagacg aagacgtccg ccgcccggct ccccgaggcg ctccgcctga ggagcgcctc
    19981 gagccgcgcg agaagctccg ccatctcgaa gggcttcgtg aggtagtcgt cggctccgag
    20041 tttcaggccc acgaccttgt ccacgacctg ggcccgggcg gtgagcatga ggatgggcag
    20101 cgcgaggccg cgctggcgca tttcccggca cacggcgaag ccgtccacgc ccgggagcat
    20161 cacgtcgagg atcactacgt cgaacggctc ctcggcggcg acccccagcg cggattcgcc
    20221 gtcgccgcgc gccgtgacgc ggtagccctc ggccacgagg cggtcggtca gggtcaggac
    20281 gaggctctcc tcgtcctcca cgagcaggac tcgcggcgcg atgcgcttcg gcgcggctgc
    20341 gctcacacgt ccgtccccgt cccggcgtct gccggcaggt ggatcgcgaa gacgctgccc
    20401 ttgccgggcg tcgaatccac cgtcacgcgg ccgccgtggg cctccacgac gtggcgcacg
    20461 acggcgaggc cgaggccgct cccggtgatc cccgcggcct gggcgtccgc gctccggaag
    20521 aacggttcga acagccggga gaggtcgctc cgcttgatcc cgatcccgcg atcggcgacc
    20581 gacacgacca cctcttcgcc ctggtgcgtc gcgacgctct tcgcgccgac gccgatccac
    20641 cccgccgttc caccgtactt cagggcgttg tcgacgaggt tcgcgagcgc ctggcggagg
    20701 gccgcggcgt cgccgcgcac accgggcagg cctcccgcca ccgccttctc gaccgcgacg
    20761 cgtttctcct cgagcaccca ccggcagtct gcaagaacgc cgtccacgag cgcggcgacg
    20821 tccacgggct ccatccggaa actctgcacg cccgaccgta tcccggcgaa cgtcaggacg
    20881 cggcccacca tctcggtgag cctccggcct tcccgctcga tgagcgcacc gtatttccgg
    20941 accttctcgg ggctgtcgac gattccgtct gcgaggttct ggccggccga gcgcatcgcg
    21001 gtcagcggcg tcttcagctc gtgcgacacg cccgcgacga actccatctg ctgacgcgcg
    21061 agccgctggg cccggcgcgt ggtcgcgacg aggacgacga gcgtcgccgc gaggagcgcg
    21121 aggatcgcgg cgctgacggc gaggtttcgg cgccgcgcgc cggccacggc ggcctccagc
    21181 gaaccggacg ggtggcgcac cgagaggatc cacttcccgg gctccggttc gtcaccgggc
    21241 cggtgcagcg tgaagaattt ccgcaccgcg tcctccggat gcacctcgcg ggacgcgacc
    21301 cgcggccctc cctggaacag cacgtcgtcc ggccgtccgg cgacgacgac cgagaccgaa
    21361 aaggcgaggc cgcccgggcc gccgaattcc cgctcggccc ggcggagaag gaattccttc
    21421 tgcaggaatt cgcggtccag gcggacgacg acgtgctgcc gcggcggccg gcgttctccg
    21481 cccgcgtcgg gttccgggtc gcggggagga ggcggcctgc gctcgtcctc gccgccccgc
    21541 cgccgggaag gtcggaccgg aacgatgacg cccggcagcc gctcgtcgag cgaaggcacc
    21601 ttcccgcgcg cctcgaacgc ctcgcgcacg gactcgagtc ccgcctccca tgccgcgtcg
    21661 ccgaggcgcc cgctcgcctc gtcgaagcgc tgcagggcga tctcccctct tccgaatcgc
    21721 gtgacgacga gaagctcgcg cacgaacttc ggcgcgggag cggttgcgcg ccagcgtgcg
    21781 aggcgaccgg agaggtcgtt cgcgagttcc tcgtccggaa tgtcgtcctt cgactggaac
    21841 gcctgctgaa cgcgtgcaat ctcgcggtcg acctcggagc agaagcgctc ggccgcgtcg
    21901 tcgagaaccg tcttcaggtg ctcctgctcg gcttcgctga cgcgcgcgat ccaccggtac
    21961 tgcagccctc cgagcgcgac gagcgcggcg agcaatccga ggatgagggc gatcggcaga
    22021 agcgaaggca gtttgcgcgg ccgcatcaga ccaagtgtag tgccaaaaac ccgcctgcaa
    22081 tcaacacttt acatctcttg caactctctt acgggccggt gatccggggc atgcactggg
    22141 ccgtccgctt cccttgacac ccattcggcg ccgccccatg tctatggagg agggtctgaa
    22201 tgaatgcctc cgcggaggcg ctcggcgcct cgtcccgcgt ggtcgaggcg cgtgagattt
    22261 ccgcgctcct cgacgtcatc ctcgaatcgg gttaccgggt cctcgggccg accgtcaagg
    22321 acggcgcgat cgtgtacgac gagatcgcgt ccgccgcccg gctcccggcc gggatcggac
    22381 aggaacagac gaacggccgc taccgcctcc gaaagcgaag cgacggagcg ctcttcggct
    22441 acaccgtcgg gccgcacgcc tggaagaagt tcctcttccc cgcttccctt cgcctcttcg
    22501 gcgtcacccg ccagaagaag acgctccagg tcgaacccgc tgcggcggac gcacggcccg
    22561 acgccttcct cggcgtgcgg ccctgcgatc tcgcggcgat cgcccgccag gaccgcgtct
    22621 tcctcggagg gccgtacgcc gatcccgcgt accgcgcccg gcgcgagaag gcgctgatcg
    22681 tcgccgtgca gtgcggcgag gccgagggca cgtgcttctg ctcctcgctc ggaacgggcc
    22741 cgcgcgccgc tgcgggattt gacctcgctc tcacggagct tctcggagac gggcggcacc
    22801 agttcctcgt cgaggtccgc tccgaccggg gcgcgaacct cctccaaagg atcgactcgc
    22861 gccccgcgag accggacgac ctggaggccg cgcgcgcggc gacccgccgc gccgaggccg
    22921 ggatgggccg caagctcgac acggcgggcc tcaaggacct tctgaccgcc agcaacgaca
    22981 gtccccgctg ggagcagatc gcggagcgat gcctctcgtg cgcgaactgc acgatggtct
    23041 gtcccacctg cttctgcgcg accgtcgagg acgcgtcgga cctcacgggc acgtccgccg
    23101 agcggtggcg gcgctgggat tcctgccaca cgctcgactt ctcgtacatc cacggcggca
    23161 gcatccggcg cagcgcgtcg gcccgctacc ggcagtggat gacgcacaag ctcgcgacgt
    23221 gggtcgacca gttcggcgag accggctgcg tcggctgcgg ccgctgcatc acgtggtgcc
    23281 ccgtggggat cgacctcacg gaggaggccc gcgttctcca ggaggcccgg cccgcgccgg
    23341 ccgcctgacg gaggaaccga tggaagctct cgaccccctg ctcgccgaac acccgttcct
    23401 caagggcctg actcccgcgc acatccggac cgtcgcgggc tgcgcgcgca acgagcgctt
    23461 cgaccccggg cagttcctgt tccgcgaagg cgaggaagcg catcagttct tcatcgtccg
    23521 gcacggaaag gtcgccgtgg aagtcggggc tccgccgcgc ggcgccgtga cgatccagac
    23581 cgtcggcgac ggcgagatcc tcggctggtc ctggctcgtg cccccgtacc gctggcgttt
    23641 cagcgcgcag gccaaggagc tcacgcgtgt catcgcgctc gacgggcgct gcctcaggca
    23701 gaagtgcgag gaggaccacg acctcggcta cgagctgctc aagagcttcg ccgacatcgt
    23761 cgggcagcgc ctcgacgcga cgcgcctgca gctcctcgac gtctatgcca ccgagccggc
    23821 cgggagacac aagtgagtgt ggtcctcgag gccccacggc ccgcggacgc ggctccggga
    23881 ccggcggcgg acccgatgca gcccgtcccg ttcgtcgtgg agcgcacgca gcgcgagacg
    23941 gccgacacgt tcaccctcga gctcctcccg cagggcgggg gccggttttc gttcgatccc
    24001 ggccagttca acatgctcta cgtcttcggg atcggcgagg tgccgatctc gatcagcggc
    24061 gacccggcct tcccgcagcg gctcgtccac acgacgcgcg tcgtgggcgc cgtcacgaag
    24121 gggatgggcg ccctgaagcg cggcgacgcg ctcggcgtgc gaggcccgtt cggcacgggc
    24181 tggccggtcg aagaggcgga gggctgcgac gtcgtcttcg tggcgggcgg catcggcctc
    24241 gccccgcttc gcccggccct ctacgagatc ctcgcccacc gcgagcgatt cgggcgcgtc
    24301 gtcctcctct acggcgcccg cacgccggac gacatcctgt accgcaagca gctccacgac
    24361 tggcgcgggg atttcgacct cgacgtccac gtgacggtag accgggcgac gagcgcgtgg
    24421 cgcgggaacg tcggcgtcgt cacgacgctc atcccccgcg tcccgttcga cccgtaccac
    24481 accgtcgcga tggtctgcgg ccccgagacg atgatgcggt tcaccgtcct cgagctcgag
    24541 cgccgcggcg tcccggccgc gaaggtgtgg ctctcactcg agcggaacat gaagtgcggc
    24601 gtcgggctct gcggccactg ccagtgcggg ccgaagttca tctgcaagga cgggccggtc
    24661 tttccctgcg ccgacgtgtg ggacctcatg tcccgccggg agctctgaat ggagaacgca
    24721 aagcccacgc tcgccgtctt caagttcgcg tcctgcgacg gctgccagct ctcgctcctc
    24781 gactgcgagg acgagctcct ggccgtggcg ggcgccgtct cgatcgacaa cttcctcgag
    24841 gcctcccgcg ccgtcggcaa ggggccgtgg gacctcgtcc tcgtcgaggg ctcgatcacg
    24901 acgccgcacg acgccgagcg catccacaag atccgccgcc aggcgaagtt cctcgtgacg
    24961 atcggcgcct gcgcgacggc gggcggaatc caggcgctcc gcaacttcac ggacgtcgcg
    25021 gactacgcga aggccgtcta cgcgtcgccc cagttcctcg aggtcctcgg caagtcgacc
    25081 ccgatccgcg accacgtctt cgtcgacttc gagctgcgcg gctgcccgat cagcaaggtc
    25141 cagctcctcg aggtcgtgag cgccttcctc gcgggccgca agcccgtgac gccggcctcc
    25201 agcgtctgcc tcgagtgcaa gcgccgcggc acgccgtgcg tcatggtcgc gcacggcacg
    25261 ccctgcctcg ggcccgtcac gcaggcgggc tgcgacgccc tttgcccgtc ctggcaccgc
    25321 ggctgcttcg cctgcttcgg gccgatggag tcgccgaaca ccgcgtcgct cgccgctctc
    25381 tggggcgagc tcggcgtccc gccggccgcg ctcgtgcgcg cgttccgttc gttcaacgcg
    25441 agcgccgagg cgttccgcaa ggagagcgag ctccatgagt aaggtccgga cgatcacggt
    25501 gaacgccctc gcccgcgtcg agggcgaggg cggactcaag gtgcgggtcc gcgcggggaa
    25561 ggtgcaggaa gtgaggctcg acatctacga gcctccgcgc ttcttcgagg cgttcctcaa
    25621 gggccgcgcg ctcggcgagg cgccggacct cacggcccgc gtctgcggaa tctgccccgt
    25681 cgcgtaccag atgagctccg tccacgcgat ggaggccgcg tgcgcggccg accccggccc
    25741 gcaggtgcgc gcgctccgcc gcctcctcta ctgcggcgag tggatcgaga gccacgtcct
    25801 ccacgccgcg atgctccacg cgcccgactt cctcgggtac gagagcgcgc tccacatggc
    25861 caaggaccac cccggggcgg tcacgctcag cctgcgcctg aagaaggcgg gaaacgagat
    25921 cgtgacggcc gtcggcgggc gcgagatcca ccccgtgaac gtgcgcgtcg gcggcttctg
    25981 gcgcgcgccg tcgccgtccg agctcgcgcc gctcgtcccg aagctcgaga cggcgcgcgg
    26041 cgaggcggtc gagctcctga gatgggcgtc gggcttcacg tttccggacc tcgacctgca
    26101 ccacgagctc gtctccctgc gccatccgtc cgagtacccg ctcaacgagg ggcgcatcgt
    26161 ctcgagcgaa gggctcgaca tcgccgcgtc cgagtgggag cagcacttcg aggaagagca
    26221 cgtccagagg tcgaacgcgc tccacgcgcg gcgcaagggg ggcgggatca tccagaccgg
    26281 tgcgctcgcg cgctacaccc tcaacttcga ccggctgccc gcgcccatcc aggccgccgc
    26341 gcaggaggcc ggcctcggga aggagtgccg gaacccgttc aagagcatcc tggtgcgctg
    26401 cgtcgagacg ctctgggcga tcgacgaggc gctcgtgatc ctgcgcgcgt acgagccgcc
    26461 cgacccgccg ttcgtcgacg ccgtcccgcg cgaagccgtg ggagcggcgg ccaccgaggc
    26521 gcctcgcggc ctgctcttcc accgctaccg catcggcggc gacggcctca tcaaggaggc
    26581 ccgcatcgtg ccgcccacgg cgcagaacct gagggcgatg gaggcggacc tgcgcggctt
    26641 cgtccaggac cacctcgaca ttgccgagga ccggctgact ctcctctgcg agcaggtcgt
    26701 ccgcaatcac gacccgtgca tctcctgcgc gacgcacttc ctgaagctca ggatcgagag
    26761 ggactgacgt gccgctcctc gtgatcggcg tcggcaacga cttccgcggc gacgacgcgg
    26821 cgggcctcct cgcggcccgc cgcctgcgcg cctgccgcgg cgtgcgctcc gtcgagcaca
    26881 cgagggacgg gctctccctc atggagcatt ggcgcccgac ggaccgcgtc gtggtcgtgg
    26941 acgcgctctc gtccggccac gctccgggga cggtcttccg cttcgacgcg acggagggtc
    27001 ccgtccccgg cgacgtgggc tggatctcgt cgcactcgcc cggtgtcgcc gagggcatcg
    27061 agacggcccg catcctcggc cgcctccccg cctcgctgac ggtctacggc atcgaggccg
    27121 agcacctcga cccgggcgcc cgcgtcacgc ccgcggtcgc gggagcggtc gaggaagtcg
    27181 ccctcgagat cctcgaggat ctgaaccggc cctgacggga cggcccgcta ctttccgagc
    27241 tccccggcca ccttcgccgc cgtctccacg ccgcgcgcga gcaccgaccg gatgcgcccg
    27301 tcctccagcg cgaggattcc ggcgatcgtg cagcccgccg gcgtcgtcac gtcgtccttc
    27361 agcgccgcgg ggtgccggcc cgtcgtcagg accatctcgg cggcgcccat cgtcatctgg
    27421 gcgacgagcg tcgtcgcgac gctgcgcggc aggccgcgca tgacgccgcc gtccgcgagc
    27481 gcctcgatga tgatgtagat gaaggcgggg ccgctcgcgg agaggcccgt gaccgtgtcc
    27541 atgtgctttt cttcgagctc gaggaccttc ccgagcggct cgaagatctc cgtcgcgagc
    27601 gcgacgtcgg catcggtggc gtggctcccc ttcgacagca ccgtcatgcc cttcccgatg
    27661 aggcacggcg tgttcggcat ggcccggagc acgggcgtcg cgtcgccgaa gtggccctcg
    27721 atgaaacccg tgctgatgcc ggcggcgatc gagatcacga gcggcggcct cctgcgccgg
    27781 ccccggatcg actcgacggc cttcgccacg tccctcggct tcacgcacag gaggacgatg
    27841 tcggcgtcgg cggcggcttt cgtgttcgcg gtcgtgcacg cgaagcccaa ctcctttgcg
    27901 accttctccg cgctggcctt cgtgcgcgcg gtcgcgcgca gcgcctgcgc cctgaccttt
    27961 cccgaacgca gcaggcccga ggcgatgctg cgacccatgg ttcccaaccc gatgacggcg
    28021 atcttcttcc gcgcgagcat gttcggactc cttcggtgat gcgcggagtt tacggcgtcg
    28081 ccgccatgcg acggaggcgt gagagtagat tccgttccca tgaacgggag cggctcatcg
    28141 tcaggccgcc agttggcgct tctcgccctc ttcttcgcct ccgtcgcggt cttcgcggat
    28201 ctctacgtga cccagccgat cctcccgctg ctctcgcacg agttcggagt ctcagctccg
    28261 acggccgcgc tcaccgtctc ggtcgtcgtc ctcatgatcg cgctcgtctc gaacgcctac
    28321 ggcccgctcg cggacgcctg gggccgcaag cccgtcatgg tcgcgagctg cgcggcgctg
    28381 gccgtcccga cgttcctctg cgcggcggcg ccctcctttc ccgtcctcgt cggcctgcgc
    28441 gccctgcagg gtctcctcat gcccggcgtc gcggcggtgg ccgtcgccta catcggcgac
    28501 gcgtacgccg gggccgccct cgggcccgcc gttggcagct acgtggccgc ctcggcgctc
    28561 ggcgggctca cggggcgcgt cgcgagcggc tggatcgcga gccatttcag ctggcgcgcg
    28621 ccgttcctcg tcttcggcgc cgtgacgctc gtcgccgcgc tcggaatggc tctccatctc
    28681 cccgcttcgc cgcccgggcc cgcccgctcg gtggcgggcg ccttccgcga gatggccggg
    28741 cacctgcggg accggcggat cgccggcggg ctcgtgatcg gctttacggt cttcttcggg
    28801 ttcatcgcga tcttcacgta cctgccgtac tacctcacgg ctcccccttt cggcctctcg
    28861 acggcgagtg tctcgggtgt ctacctcgtc tacgtcgccg ggatcgccac ctcgtggctg
    28921 gcgggccggg cgtcgcagcg gttcgggcgg agggcgatca tggccgccgg cttcctcgtg
    28981 gcggccgccg ccgtcctgtc gaccctcgcc gcctcgctgc ccgcggtcgc atgcggcctc
    29041 gtggccctct gcatcgggat gttcctggtc cagggcaccg ccccggccta cgtgaacgcc
    29101 gccgcacggc aggccaaggg cggggccggg gccctctaca cgacgtttta ttacctcggg
    29161 gcgacgttcg gctccgtcct gcccggattc gcctggcagg cgtggggctg gccgggggtc
    29221 gtcgccgcct gcctggcctc ctttgcggcc gggctgctcg cagtgaaatt tctctgcgcg
    29281 taaatgcgcc ggaccctccg gcaaatgatt gacaggtcgc cccgttggac tatcctttac
    29341 ctacgcctaa cgaccggccg aagaggagac tcgttctcat gccgatttgc gcgcaccccg
    29401 caccccggag gctcgcttcc gttctcgcca ctgcgtccct ccttgccctg atcggcgccg
    29461 ccttcccggc tcacgggcag gccatggtca aggtcaacga caacgtcaac ttcaagctcg
    29521 gattcctgat ccagccgcag gcggactgga ccgagaccat caacgcgacc ggcgacggat
    29581 cgaacgggtt ccagcagaac ctgctcatcc gccggctgcg cttcctcatc ggcggccagg
    29641 tcgcgaagaa cgtgttcttc ttcgcggaga ccgagaactc gaacctcggc aagtccacgc
    29701 aggccgtcgg ctcgacgacc ggcacgaagt cgccgggcac cgggttcaac ctcctcgacg
    29761 cggtcggcga gtggcgcatc gcgaaggagt tcaacgtcca gttcggcgag ctgcggtccc
    29821 cggtcagccg cgagggcctc aagagcagcc cgaaccagtt catgctcgac ctctcggcct
    29881 acgcgttcct gacgagctcg gctctccaga acaacgccgg ccgcgacacc ggcgtcatgt
    29941 tccgcggctg gttcctctgc gaccgcctcg agtaccgctc ggcgatcgtg tcggggctcc
    30001 gccagcccgg cgtcaagaac tcgccgcgct tcgtgaaccg gctccagtac aacttcttcg
    30061 acacggaggt ctacaacctc ccgtcctacg cgggcgtgaa ctacggcaac aagaagatcc
    30121 tcgcggtcgg cggcgcctac gacacgcagg gcgcctaccg gttcgcctcg gccgacatgt
    30181 acctcgactt ccccgttccg gtaggctcgt tcgagtcgac gatccagtac cagtacgcga
    30241 acggcggaac gttcatcacg acccttccag agcagagcac gttccagatc gaggcgggcg
    30301 cgtacttcaa gaaggccaag atcgccccga tcgcccgcta cgagcagaag acgttcagcc
    30361 agaccatcaa cgagtcgaag aacgagtacc ggtacgcatt cggcctgaac tactacccgt
    30421 acgcgaagtc cgagagcaac ttcaacatca agttctggtg gcagcgcgtc acggtgaagt
    30481 gcgcgtcttc cgtgagcccg tgcccggcca gctcgtcgaa cttcatcacc gaccaggtca
    30541 ccctccagat gcaggcctac tacttctgat ccccggcgcg cccgcgccaa ccgaccgaaa
    30601 ccccccgccc ctctcaggag gggcggttcc gttctggagg acacgatgac gccgaaccgc
    30661 agctcccgcc ttctcgccgt cctcgccgcc gcgcttctcg cggtcttccc cgcccgctcc
    30721 gaggacgaga tccgcctcgg cgccgtcctg cccgtcaccg ggaaggagtc gaagatcggc
    30781 ggcgcctaca agcaggccac cgagttcgcc gtcaaggaag tcaacgacgc cggcgggctc
    30841 gacgtcgggg gcaagaagat gaaggtcgac ctgcgcctcc tggacgacac ctccgaggcc
    30901 agcaagagcg cccagctcgt cgagcagctc atggcccagc agaaggtcca cgccgtcgtc
    30961 ggcgggtacg gctcgcagct cgtccaggcg cagagcgtcg tgccggaccg ctacgagatc
    31021 ccgtacgtcg cgggcggcgc gggtgcgacg agcatctacg ggcggagcaa gtgggtgttc
    31081 ggcacgctct cccccgtcga gaacctcgcg acgacgcaga tggagttcct ctcggacctc
    31141 gtcaaggagg gcaagctcaa gccgcccctg aagatcgcga ttgtctggga gaacaccgag
    31201 cacgggaagg acttccagaa gggcatccac gacttcgtca aggcgcaccc gaaggagttc
    31261 tcggtcgcgc tcgacgagag cttcgagatg tacgcgcccg acttcaagcc gctcctgacg
    31321 cgcgtccagg cggccaaggc cgacctcttc atgtgcgacg cccacctcga ggactacatc
    31381 tcgatgcagc gcacctacac gcagatgggc ctctaccacc tcatgatcac gtacggcgcg
    31441 cgcggctcgg acgaggccgc ccgtaagggc ctcggcgccg ccacggactg gatcttcgcg
    31501 agcggctggt ggtcggacct cctcccctac ccgcaggtca aggcgtggac ggagaagtac
    31561 aaggccgcga ccggcacgac gccgcagtgg taccacgcgt gcgcctacga gaccgtccgc
    31621 gcggtcttca cggcgatcaa gaaggccggc tccctcaagc cggaagcgat ccgcgccgcg
    31681 ctcgcctcgc tcgagatcaa ggactcgatc ctgcccggcg gcgtcttcaa gttcagcaag
    31741 accggccagg ccgtcctccc gttcgtcgtc acccagaaca aggcgaacgg caagctcgac
    31801 ctcgtctggc cgaaggacgc ccgcacgggc ccgccggtcg caccgatccc gaaggggtga
    31861 cggggggtag cgcatgccgg gcgggaaccg cttcgcgggt gtcttcgaca ccgccgtcgg
    31921 gtccacgatg cggcccggag atccgggcac ggccaagctc acgcgcgact ggggcgaacg
    31981 gctcgtctcc gtccgctacc gctacgcggc cgacccgccg atgcgattca ccaccatcga
    32041 gatcgtcgtc gacgcggcgc cctggaagcc ctcgcccgcc cggcccgtgc tcgtggaggt
    32101 ccgctcgtgg gagtcggctc tccggaagag aatcgtcgag gcgggcggcc aatgggacgc
    32161 gcgggccgga cgctggcgga ttcgatacga caaggttctg gccctcgatc tcggagcccg
    32221 ggtgcagctc acgaagaagg caaggggccg gacgcggagg tcgaaaatct ctacaccgga
    32281 agggacaccg acttccctac ctaagccgaa gccacctcga aatgtaggca ggggcgccta
    32341 gaaatgcgcc gcccggatgc cgtctaatac tagttaggcc gcagaaacct tcatgagcga
    32401 acaccacgtt ttcgagtcgg cgctacgcga tgtcgcgttc gcaccgaacg gcgcgtttcg
    32461 tatccgaccc accggcgccg acatcgtggc ggcttttgtc gtaaccctgt tcggtgcatc
    32521 ggcctctcta ctcgggggcg tcttcgctct cgtgttcctt gcaaacggct ggtcgtttcc
    32581 actggtcgaa gcgcagggca tggccttcct cgtcgtcgcg cccgttctct tcgttttgtc
    32641 ggccgctcca ctagttctgc atcgcgcgcg cctccttggt gcgggccagg gcgtgcgcaa
    32701 tctcgtgttt ggcgtcctgc tcagcgtctt ttcctttccg cttctcatcg tcgccactaa
    32761 tgcttcgccc tcgcttccag cgtggcttcg ggtcgccggc gttctttctg cgggctttat
    32821 cggcggcctg gcagctcatg gcgtctcgct gctgcatagg cgggctgcgg cctaacacct
    32881 cattgcagcg gactcgtcgt cagtcgctgg cgctccttcc ttctcgccgc tgaactcgat
    32941 atcgttagac gccgagagtg gagagcacac gtcttgagcg cctacgtgcg gcggcagaag
    33001 ccttccggcg cgcgctcttg gccgtacctg gcctcgacct gcctgtaacg ctgcgtacct
    33061 ttccggtcgg cgcgtgcggg gacacttgcc tcctacttgg cgcgtacctc cacgacctcg
    33121 gcctcggccc gttcgcctac gtaaacggct cacgcccggt agagcgcgcg cctggcttgt
    33181 tgcaatcgca cgcatggctc gcttcaaccg acggcacggt catcgacata accgcgcacc
    33241 agttttcgga gtttccggag ccggtgtacg tgggtgcggc caccgcctgg cacggcactt
    33301 tcgattcggc tcagcagggc acggctgact ttcggaatta cgacccacgt acggtagagg
    33361 cgctcgggcg cgtctatgcc gccatccgca gtcgcgtcgg cgtctaacac ctcattgcac
    33421 cggactcggc gtcagtcgct gcgctccttc cttctcgccg gtgaactcga tatcgttagg
    33481 cagcacaccc tctatgccag gcaacgaact tggccccgac tccaaagcgg aatgcccccg
    33541 tcacggcatc cagcgcccca cgttcgtctg catccacata gtcgaacaca ccgcagtttc
    33601 cttcgtgacc ccgtccggac cgcccgaccc tttcaaggaa gcatggtgcg agcgctgcga
    33661 cgcgcttgtc atggcggatg gagagtggaa cgaaaggtcc gaggcgtttg cacgcatcac
    33721 gatgatctgc gaaggctgtt acgaagagcg tcgcacggct ctccagcccg gcgcctgacc
    33781 cccacccttc cttccagccc aagtaaggcc agtcgcctca atcagtcctc acattccctc
    33841 agccgcacgg cgcccgtgcc gcctaacaac tcgttgcagc ggactcggca gttgacccga
    33901 taaagtggac acttcggcct aacagccagc gaggtgtcga tggccagaat caagggtcca
    33961 cgaagggata aactgaacac gaaggagcgg cacatgcgaa ccctcacgct ggcgctcgcc
    34021 ctcgtcttcg cggcacctcg tcctctggct gcggaccaaa cctgcgttag aaccattggt
    34081 ggccggcaca ttccagcgtt cgtcgggctc ggttcggaag ggctcgattt ccagaaacgc
    34141 tgtctcgtct gggaatactc cgccgagccc aaagctgagt tcatcccgtc tccggaaacg
    34201 ccgtccttgg gttcgcacgc gcaggttgtc gtcatcgaac ctgctcctga ccgctcgtcg
    34261 ggctcaatta ccctctctga gcttctttcg cacagcctga gcctgaagcc agttgcttcc
    34321 gaagccgcgc tctcgcttat gccagcagtt tccgacgcgc accctctcac agccaagtag
    34381 cgggcatcgg agcgccgccc atgagtcgag cccgcatctc cgagatcgca ccgttcttca
    34441 tcgtcaagga cgttgccgct gcgctttcgt tctatcgcga cgcgctcggt ttcgaggtca
    34501 cctttcagga gcccgctcaa gacccgttct tcgcgatcgt cgcccgcgac ggcgcgatga
    34561 tcatggtcaa ggccgtcggc gtcgcgccgc ttcccaactg ccaacgtcaa cgggacgctc
    34621 gatgggacgc ctacctcgcc gtgcctgatc cggacgcgct ggcggcggaa ttcgcgacgc
    34681 gaagcgtcac cttctccgtg cctctcgaag acacgcatga cggcctgagg ggcttcgagc
    34741 tcaaggacgc cgacggctac gtgctgttct tcggtcgtcc tcgaatctag acgacgctcc
    34801 ttccctctcg ccgctgaact cgatatcgtc gggcggcacg acctgccagg gaggaccgtg
    34861 tggcgaactc aaagcagatc gcgggcctga tcggcccggc tctcgtcgcc atgctggtct
    34921 ccgagtttcc cctcgtccag ccgcacctgt acgacagcca gatcccgccc gtggtctacc
    34981 tgtcgggtgt tctcatgttc gtcggcggtc ttgccgtcgt aaggcagcac aacgcatggg
    35041 cgcgcaactg gaccgtgctc gtcacgctca ccggatggtt cttcctgctt ctcggcctgg
    35101 tccgcatgtt cgccgccagc cagtaccatc aggccgcatc gagcacgagt tcgaccgtct
    35161 tcatggcgct cgagggcatc ctgttcgtcg tcgccgtcat tctcacgtat caggcctacc
    35221 gccgagaacc gcgaaaggag ccgtgagcac atgaagaagc cgggcgcgag ccgggaacca
    35281 tcggcatcgg atctcatctc gaagcgaatc gcggagctcg gggactggcg cgggaagacc
    35341 ctcggccgga tgcgcaggct catcagggag gcggacccgg acgtggtcga ggagtggaag
    35401 tggatgggca ctcccgtctg gtcgcacgac gggatcatct gcaccggcga gtcctacaag
    35461 agcgtcgtga agctcacctt tcacaagggt gcgtccctga aggacccggc ccgcctgttc
    35521 aactcgagcc tggacggaaa cgtccgccgc gcgatcgaca ttcacgaggg agacgacgtc
    35581 gacgcgggcg ccttcaaggc gctcgtccgc caggccgtcg ccctcaacag ctcccggccg
    35641 tccaaacctc cgaagaaaag gaagacatga acctcgccgt cgcctttctc gcgctcgcgg
    35701 ccgcgttacc ttctccggcg gacggcggcg tccccgtcga gcaggagccg gaacacagga
    35761 cggtcttcaa gaacgactac gtgcaggtct tccgcgtgac cctcgcgccc gggaaggcca
    35821 cggggatgca cgtccacgcg cacgacgacg cggccgtccg cctgagcgcg gcgaccgtga
    35881 cctcggacag ccccggcatg ccggcaggcg ccccggaacc cagggcagca ggattcgtgt
    35941 cggcccggaa caacgagccc agggcgctca ctcaccgggt ccggaacgtc gggaccaccc
    36001 tcttcgacgt cctcgacgtt cagatcctga agcggcccgc gggccccgca gcgccgccgt
    36061 cctcggcgcc cgcggccgag aacccgcaga tgcgggtcta ccgctatgag ctcgcgcccg
    36121 gagccggctc ggcgcagcac gtccactcgc gcccgtacct gctcgtcgcc gcgacggaca
    36181 tggacctgcg catgacggcg cccgacggcg cctccatggc gcacccggtc aaggccggcg
    36241 acctccactg ggtcgagtcc gccgtcaccc acacgttcgt gaacgaaggc aaggcgagcg
    36301 gcgtgctcgt cgaattcgag ctgaagtgaa gcgatcacgc gcgcgtcgaa gatagtcatg
    36361 cggtttccgc tcccgcccgc catacgtctc aggagtcaca catgacgtcg atccgcggga
    36421 aggcccgcat ggccgggttt ctctacctgc tgctcatggc ggcgcccctg cgcctcctct
    36481 acatcccgaa gacgctcttc gtgaaggggg acgcggccgc gacggcggcc aacatcgcgg
    36541 ctcacgaaac gctcttccgc ttcgggatgg tcggcgacct cttctgcggg gtcgtgctga
    36601 tctttctcgt cctcgcgttc taccgtctgt tcgaggcggt ggaccggacg ctcgccgtgc
    36661 tcgtggtggt cctcggcggc ctgctgcccg cgacgatcga cttcgtcaac gtggtgaacg
    36721 acgcggcggc cctcatcctc gtgcgggggg ccgactttct gtccgtgttc gacaagcccc
    36781 agcgcgacgc gctcgccatg ctgttcctgc gcctgcacca ccagcaggtc gtcgcggccg
    36841 agacgctttg ggggctgtgg ctcttcccgc ttgcgctcct cgtcctgaag tcgcgcttcc
    36901 tcccccgctt cctcggctat tggctgatcc tgaacggctt cgcctatctc gcggagagcc
    36961 tcacgggcct gttgctgccg cagtacgagg cgactgtctc aaacgtcctc ttccccgcgc
    37021 ttctcgggga ggtcgcgttg atgctttggc tgctgatcag aggcgccgac gcgggggcgg
    37081 catactccgg aacctgatcg aggagatgga ggatcgacgt gggcacaggc agactcgagg
    37141 cgttcagcga cggcgtcctc gcgatcatca tcaccattat ggttctcgag atgaaggtcc
    37201 ctcacggggc ctcgttcgag acgctttccc ctctcctccc ggtgttcctc agctacgtgc
    37261 tcagcttcgt ttacgtcggc atctactgga acaaccacca ccacatgctg cacacgtgca
    37321 cgagggtcac cggaggagtc ctctgggcga acctgcatct cctcttctgg ctttccctgt
    37381 tcccgttcgc cacgggctgg atgggcgaga accatttcgc ctcgatcccc tcggcactct
    37441 atggcgcggt cctgctggcc gcggcgatcg cgtactgggt cctgcagcag gtgatcatcg
    37501 cgtcccaggg cgagggatcc gccctcaagc gggccgtcgg cggcgactgg aaaggcaagg
    37561 tctcgccggc cctgtacgtc tgcgccatcg cctcgacctt tttcgttcca tggctcgcgc
    37621 aggcccttta cgtcgccgtc gcggtcctgt ggctcgtccc cgaccggagg atcgagcggt
    37681 cgctcgcgag agcggactga aacggagacg ggccggcccc ggcacggttc aaggcctaga
    37741 ctcgctccag gagacctcga tgcccacgcc caagacgtcc cttcgcttcc cgctcccgcc
    37801 cgccctgcgc acccgcacgc tgaagctcct gacgaagctc gacggcgacg aggatcccac
    37861 gcggcacacg gacgacctgt gcgcgctcgt cctcacgctc accgagtccg ggatggccca
    37921 ctacttcctc aaggccgtcg gcgacgcgaa gctgggcttc gtcgcgcgcc agaccgccag
    37981 tctcggcgtg tcgggggcgg tccgcgtgat ggcgccgatc gtccggtccg tcctcggcgg
    38041 cgccgacgcc gcgcagctca gggccgtgtc ccggcacata cacggcctga tggcgtgaag
    38101 gccccccgat tcgctagagt cggcgcgtga ccgagtcccg gcgtctcctc cggcacttcc
    38161 tcgcggccct cgcctaccgg acccagaagg cgctccgggg cgccccggcc ggcttcgggg
    38221 agttccgcgc gggcgtgaac gtccggacgc cgcacgagct cgtgtggcac atgacggggg
    38281 tcgtcggcta cgcgcgcacg atgcttcacg ggggcgattt cgcgccgccc cggctcgcca
    38341 cgttcgagga cgaggtcgcc cggttccacg cgacgctcga ggcgctgcgc gacgacctct
    38401 cggaccccct cctcgaggcc cggatctccg acgagcagtt cctgcagggg ccgctctcgg
    38461 acgccatgac gcacgccggc cagctcgcga tgctccggcg cctggccggc gagcccgtcc
    38521 cgtccgagaa cttcatcttc gcgaaggtgt cggccgacaa cgtcggcccc gaccagcccg
    38581 agccggccgc ccccgacgcg tggtggcggg cggaccagcc accccagccg ccgggaccgc
    38641 cgaagaagga gtagggggcg tcgaatgacc gtcttcgaga agggcttctt cacgatcctc
    38701 ggcctctcgg tcgtcctgat cctcgtggcc atcccgctcg cgctcggaaa ggtgccgccc
    38761 aacgtcgtct acgggtaccg tacgcgcgcg acgctcgccg acgaagacgt ctggtacgac
    38821 gcgaacgcgt atttcggacg ccgcctgatc gtcgcgtccc tctgcggcgt gctcgcggcg
    38881 tgggcgctct acgtcctgaa gccgttcccg ccggacgtct tcctgcccgt ctccctcgtc
    38941 gtcctcgtcg cgcccacgct cgtcgcggcc gtcgtcacgg cgcggtacgt ccgcaccctc
    39001 gggcagaccc gctagactgc ccatccggcc gccctcaagc ggcgtagaac gaacagggag
    39061 aactcatgtc ctcaaacgtc cgtgcctatg gcgcgcccgc gcccaatgcc ccgctcgtcc
    39121 ccctcacgat cgaccgtcgc gagcccggcg ccaccgacgt cgagatcgag atcctcttct
    39181 gcggcgtctg ccactccgac atccaccagg tgcgcgacga gtggtggccg ggcacctttc
    39241 ccatggtccc gggccacgag atcgtcggtc gggtgtctcg cacgggtccc ggcgtcacga
    39301 agttcaaggc cggcgatctc gcgggcgtcg gctgtttcgt cgactcgtgc ctcacgtgcg
    39361 acccctgcaa ccggaacctc gagcagttct gcctcgaggg tgcggccttc acgtacaaca
    39421 gcaccgagat ggaccgcaag acgccgaccc agggcggcta ctcgcagcgg gtcgtcgtga
    39481 aggagcgctt cgccgtcaag gtgcccgccg gtctcgacct cgccgcggcg gcgccgcttc
    39541 tctgcgccgg gatcacgacc ttctccccgc tccgccagtg gaactgcaag ccgggcgacc
    39601 gcgtcggcgt cgtcggcctc ggcggcctcg gccacatggc ggtcaagctc gccgcggcga
    39661 tgggcgccga ggtcacgatg ctcagcacgt cccgttcgaa ggaagccgac gcgaagaggc
    39721 tcggcgcgca cgcgttcgcg ctgacgagcg aaaagggtac gttcaagaaa ctggccggac
    39781 gcttcgacct catcatcgac acgatctccg tcccccacga cctcgacgcc cacctcgggt
    39841 tgctccggac cgagggggcg atggtcgtcg tcggtgtccc gcccgtgccc gcgagcctca
    39901 aggccatgtc cgtcatcatg gggaacaaga agctcgcggg ctcgatgatc ggcggcatcg
    39961 ccgagacgca ggagatgctg gatttctgcg ccgcgaagaa gatcacggcc gacgtggagg
    40021 tcatcccggc cgcgaagatc aacgaggcgt acgaccggat gctgaagagc gacgtgcgtt
    40081 accgcttcgt gatcgactcg gccagcctcg cctagtaatc tcgggacctc ttgcaggaca
    40141 tcgccggggt cctcgtcaac accgtgattt ccgcggcgct gtacgcgacg atggcgtacg
    40201 gcctcgcggt catctacggg gtcatgcgca tcatcaacct cgcgcacgcg ggcctgatga
    40261 tgctcggggc ttacgcgacg ttcgcgctct ttacgcgctt cggcctcgac ccgttcctct
    40321 cgcttctcgt cgtcgggccg ctcttcttcc tcatcggcgt cgtcctgtac cgcctcgtcg
    40381 tccgccggct gccccgttcg ggggcgacgc cctcgatgga gtcgctcctg ctgctcttcg
    40441 gcgtctggct cgtcctccag aacctcgcgt acgtcatctg gaccggcgac acgcagtcca
    40501 tcctgacgtc ctacacgatg aaatcggtgc cggtgctcgg ggtgagggtg ggcgtgccgt
    40561 cgctcttcgt cttcgcgggc agcgtcctct cgctcgtctt cctcaacgtc gtcctgaagg
    40621 cgacgtacct cggcaaggcg atccgggccg tgacgcagaa ccgcgacgcc gccctcctcg
    40681 cgggcgtgga cgccgaccgc atctccgcga tggctttcgg cctcgggacg gcgttcgccg
    40741 gcgtcgcggg cgggatgctc tcgtccctct acgccttcac gcccgatttc gggcggagct
    40801 tcctcctcaa ggccttctgc atcatcgtcc tcggcgggat ggagagcgtc acgggcgtcg
    40861 cggcgggcgc gctcgtcctc gccttcctcg agaatgccct cggctcgtgg acgacgctcc
    40921 cgacctccct gcaggacgcc gcgagcttca cgctcctctt cgtcacgctc gtcgcgttcc
    40981 cgcagggcct cccggggctc gcgacgcggg tgaggacggc gttcaagtga agcggctccc
    41041 gctcgccgcc ggcgccgccc tcgcggcctg gaccctcgcg gcgcccttgt tcctcgagcg
    41101 cggcacgctc aacgacgtct ggaacctcgc gttcgcggtc atcctcgcct cggcctggaa
    41161 catcctcggc ggcttcgcgg gccaggtctc gctcggctac tcggcgttcc tggggatcgg
    41221 cgcgtacacg acagtcctcc tgtcgctgaa gggggtcaac ccgttctgga cgctgccgct
    41281 cggcgcggcc ctcgcggcgc tgttctcggt cgtgatcggg ctccccgcgt tccgcctgcg
    41341 cgggccgtac ttcacgatcg cgacgatcgg cgtcggagag gccgtccgcg tggtggcgtc
    41401 gagcgtctcc ttcacgggcg gctcgagcgg cctcaggatg cccgcagggt ccttcgactt
    41461 cacgcgcaac tacgaggcca tggccgcgct cgcgatcgtc acggtcgttc tggcggcgct
    41521 cgtgaagagg agcgccttcg gccacgcgct cgcggcgatc aagcaggaca tcgacgccgc
    41581 cgagtcgctc ggcgtcgact ccacgcgctt caagctcgtc gctcacgcgc tgtcggccgc
    41641 gctcgtcgcg ctcgcgggct cgctctacgc catcaacttc cagtacatcg cacccggctc
    41701 cgtcttcgac ttccggctga gcctcgccat cgtcctcgcg cccatcgtgg gcggcgtcgg
    41761 aaccgtggcg ggccccgtcc tcggcgccat cgtgttctcg acgctccaga tcaagctgct
    41821 ctccgtcccc gcgctccgcg actcgtacct cttcatctac ggggggctcc tcatcctcgt
    41881 gatgctctac gagccgaagg gcctcgtcgg cctcgcgcgc cgcctcgtcg cgcgcgtgcg
    41941 gcaggcgccc gctcccccgc cggaggccgc gcgtgccgg
//
LOCUS       DAOMFY010000004        40095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_314_length_40095_cov_7.809341, whole genome shotgun sequence.
ACCESSION   DAOMFY010000004 DAOMFY010000000
VERSION     DAOMFY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 40095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40095
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_314_length_40095_cov_7.809341"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..276
                     /locus_tag="PLB01_00810"
     CDS             28..276
                     /locus_tag="PLB01_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQQ75867.1"
                     /translation="MGWIITLIIGGIIGWLASIVMKTNAQMGLIANVVVGVVGAGLGG
                     WLAGVLGIGATGSVMSWIVGIAGAAILIWILKALGVFK"
     gene            complement(337..1335)
                     /locus_tag="PLB01_00815"
     CDS             complement(337..1335)
                     /locus_tag="PLB01_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HQQ75868.1"
                     /translation="MDLRTTYLGLPLAHPLMPGASPLVDDLDTVKRLEDAGASAIVMH
                     SLFEEQITHEREGIHRAMDVHAESFAEALSYFPAPEEFHLGPDRYLEQIRKIRATVSI
                     PVIASLNGMTDSGWLSYAKLMQDAGAHALELNVYRIAADPNVPGTTIEHEVENILRSV
                     KRGLGIPVAVKLPPYFTSFANVARQLDDLGADGLVLFNRFYQPDIDVELLEPVAALQL
                     SDSSDLLLRLRWLAILSGRVRCSLAETGGVHTPIDLVKAIMAGAHAVQIVSSILRHGP
                     RQITALKQGLAEWMEKHEYESVAQMTGAVSHRTSADPSAFERANYMRVLQSFRVPV"
     gene            complement(1347..4928)
                     /gene="nifJ"
                     /locus_tag="PLB01_00820"
     CDS             complement(1347..4928)
                     /gene="nifJ"
                     /locus_tag="PLB01_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQQ75869.1"
                     /translation="MGNRRRLVCDGNTAAASVAYRLSEVVAVYPITPSSTMGELSDEW
                     MAKGLKNVWGTIPQVIEMQSEGGAAGAVHGSLQAGALTTTFTASQGLLLMIPNMYKIA
                     GELTSYAMHVSARSLATHALSIFGDHSDVMACRQTGFALLASASVQEAQDLAAIAHGA
                     TLASRIPFLHFFDGFRTSHELAKIEELTDDDLRALVDESKIDAHRARALSPDRPILRG
                     TAQNPDAFFQAREACNPFYDACAGFVVDAMAKFTELTGRAYNLFDYVGAKDAERVIVV
                     MGSGAETCDETAKWLNAKGEKVGVLKVRLYRPWSLAHFVAALPATVKTIAVLDRTKEP
                     GAPGDPLWLDVVAALAEARTTGLSKFASEPRVVAGRYGLSSKEFTPAMVASVYAEMAK
                     AAPKRQFTIGIVDDVTHTSLPWDDSIDIEPADRKKAVFFGLGSDGTVSANKNSIQIIG
                     EDAGKEAQAYFVYDSKKAGAVTTSHLRFGEAPIRAAWLVSNADLVACHQFSLLDKQDM
                     LSCVKSGGVFLLNSPYGVDEVWDQLPFEVQEQIVKKKLRFFVVDAYGLAKEIGLGGRI
                     NTIMQACFFKLSGVLPPDDALERIKKGIRKSYARRGEDVLQKNFAAVDGAAAGLKEVK
                     VPASVTTQRHMPPPVSTEAPDFVQRVSGVLLAGKGDLLPVSAFPPDGTWPTGTAKWEK
                     RNIALEIPVWDEKLCIQCNKCAMVCPHAAIRAKLYEPAALAGAPATFKSVDFKATEWQ
                     GHKYTIQVAPEDCTGCTLCMMVCPAKDKSNPKHKALDMVPQAPLREPERANYEFFLDL
                     PEADRTRLRIADVKSAQFLEPLFEYSGACAGCGETPYIKLLTQLFGDRTMIANATGCS
                     SIYGGNLPTTPYTVNRDGRGPTWSNSLFEDNAEFGLGMRLGVDHHDLTARTALKAHAA
                     EIGQQLVDEILGADQSGEAGIAAQRARVAELRKRLAASPRPEAKRLASLADYLVKKSI
                     WIVGGDGWAYDIGFGGLDHVMAVGRDVNILVLDTEVYSNTGGQASKSTPLGASAKFAT
                     AGKAVSKKDLGLMAMGYGSVYVARVAFGAKDAQTVKAFQEAESYPGTSIILAYSPCIA
                     HGYDMAFQLDQQAKAVQSGYWPLYRFDPRRTAAGEIPLVIDTGTLKGDIGAFMRNEAR
                     FRVAEERDPELYKGVVARARREAADKVLLYEQLAKKHVAEAVTRPGN"
     gene            5089..5523
                     /locus_tag="PLB01_00825"
     CDS             5089..5523
                     /locus_tag="PLB01_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75870.1"
                     /translation="MQKSHFRLWALLGGGVILVLALGGLWKVQTAPVDTTLTPAPFAL
                     PLSKTSADLQVLVAGVPLEKAIADKRLMLAGENGAAAIVVPEATVRLNEEDHARLRRV
                     PSLLALAAMAGGGLVLFLIGLLTPRIGAFKQHGLIDMHLESM"
     gene            complement(5545..5976)
                     /locus_tag="PLB01_00830"
     CDS             complement(5545..5976)
                     /locus_tag="PLB01_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75871.1"
                     /translation="MARRTEPCRKSPEERLDDLLAGHREATLRNEGAKYARRAIEQSD
                     SLPNGVKFFAWALLAADAPDEDEALAALAEAEGYLDVAREDLGRRFTRELAELRFLER
                     GIALRSDRAEFEEAVRLCDVALGLGLGTPYERRKASLLRMV"
     gene            6012..7811
                     /locus_tag="PLB01_00835"
     CDS             6012..7811
                     /locus_tag="PLB01_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ75872.1"
                     /translation="MPKKAVLALGLLLVLAAVAAYVPALKAGFVWNDDTYVTENPTLD
                     GLAGLRLIWTDTKANEQYYPMVFTSFWVEKRLWGLKPFGYHLVNVLLHAGSALLLWAL
                     LARLGLKGGAWAAALFALHPVCVESVAWVTERKNTLSLFLSLLAMLAYLAGRREKERD
                     KEKKEDFLRKGKRGRAEQRSPAAQRSPAAQPSRGARHPLLSFIGFFLFILALFAKTTA
                     VVVPAVLLVLVWWKRGEIRGKDVGPLVPWFAAGIALAAHTAWLERTVVAATGSEWSLG
                     LLGRVVLAGRTVLFYSQKFLLPIDLSFFYERWKVDASAPLQWLPTLAALALLVLAWRF
                     RERLGRGPLAFLLLFGGVLFPAMGFFNVYAMRYSWVADHFAYQAVAAASACLACGAVT
                     LLQRAPVAARRAAAAAAAGILVLLGVLTSRQARIYEGEETLWKDTLSKNPSCFSCETN
                     YGFFLVNAGRTSEAVAHFERSLKLKPDNVPALLNLGRAAEQRGSLGEAATRLRAAYAI
                     DPRDPAVLVNLATVEVKLGRAAEAIPLYEEALRLGTADAHLAHNGLGVAFMRQGRVAE
                     AAEQFREALRLRPDYDFARANLGRALAALGAAP"
     gene            complement(7815..8348)
                     /locus_tag="PLB01_00840"
     CDS             complement(7815..8348)
                     /locus_tag="PLB01_00840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQQ75873.1"
                     /translation="MKILLRSIAAAALVFAAPRPAAAEPPAVGTKAPAVSLPSQEGQT
                     VTLEQNKGKWVVLYFYPKDFTSGCTMEAHNFQRDLAKYEKANAVILGVSVDSVDSHKG
                     FCTKEGLSFKLLADTEGAATKVYDSAVVRDGKTYSARNTFLIDPAGVIRKVYLKVNPQ
                     THSDDVLADLAQLQGSK"
     gene            complement(8388..8873)
                     /locus_tag="PLB01_00845"
     CDS             complement(8388..8873)
                     /locus_tag="PLB01_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ75874.1"
                     /translation="MNTREYFVQCFKSEVPKFVRVLKAVPESGAGYRPHPRSMSAGEL
                     VWMLASELHDACEVLDKGEVSFAMGPAPATVAEAVAAYEKNAAAVKRRLAKVKDAAWK
                     KPARFLMDGKVAWKAPLGDMMWGFLFDAIHHRGQLSTYLRPMGSKVPSIYGPSADDPG
                     K"
     gene            complement(8870..9244)
                     /locus_tag="PLB01_00850"
     CDS             complement(8870..9244)
                     /locus_tag="PLB01_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQQ75875.1"
                     /translation="MKKDERVDAYLAKAAPFARPILRRLRAAVHAACPEGDETVKWGM
                     PFYTVDGGILCYMAAFKAHAGFGFWRGLDRAVLGASAKPGEGMGSLGRIERLSDLPSD
                     AKLRGWIRKAAKLRSQTQGRKR"
     gene            complement(9241..10908)
                     /locus_tag="PLB01_00855"
     CDS             complement(9241..10908)
                     /locus_tag="PLB01_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQQ75876.1"
                     /translation="MRNRLVLFAAALAGHSLFAADLVTPAQREAAGRITAEGLKADIR
                     FLSSDLLEGRGPASRGDALAEAYIQARLESLGLEPGAPGGGWIQKVPLVGIRPAYAGP
                     AAFSSPKGTATGVLGETFVAVAGDQKAASKIENAEIVFVGYGIVAPEYAWDDYKGADL
                     KGKVLLMMNNDPEGTPAEPNLFAGKTRLWYGRWDYKYLMAAKHGAAGAIVIHTTPSAG
                     YGWQVIQTSWKGELFELPDDGSPRVAMKMWATDELSKKLAALGGKDLDALRAAAETRA
                     FKPVPLGVTMSLAFTSTVQRKESGNVVGVLRGSDPKRAGEAVIYTAHHDHLGVKVAAA
                     PGGDGIYNGALDNASGVAGVLAIAQAFAELKERPARSVYFALVAGEEQGLLGSEWLAL
                     HPPVPAGRIAANINVDGIGFYGKTSDVGMIGLGKSSLDTDFTALARMQGRTVHGDEFP
                     EKGSFYRSDQFNFAKAGVPAAYVKKGTDVTGRPGLGKAMVEAYEKNDYHQPSDEFKES
                     WDFSGAVEDMRLAFWLGVRAADAAEMPRWNKGDEFEAARLKALAEAK"
     gene            complement(10949..11596)
                     /locus_tag="PLB01_00860"
     CDS             complement(10949..11596)
                     /locus_tag="PLB01_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQQ75877.1"
                     /translation="MRLIEDLRAEHVVIERTLGALRTYVEARLGGRGDPADAASFLAF
                     FRLFAGRYHHAREEDTLFPALLAHLPIRPDSGPVRSLFDQHAAMAKTLEEFAPLLARP
                     EGQGGRLLDLSTRYRRDLLAHIDAENSVLLPESEARLRRAGAPDLGGRPPDAEESAAR
                     EGAERLLALYPPSEDAGAIRGEGCVICPSYGLSCEGLEREWWTEQEWDDFRERDG"
     gene            complement(11604..11885)
                     /locus_tag="PLB01_00865"
     CDS             complement(11604..11885)
                     /locus_tag="PLB01_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HQQ75878.1"
                     /translation="MPAKKAKSVADQFKDAKARVEKLPSRPSNDQLLDLYGLYKQATE
                     GDVSGTRPGLLDLKGRAKYDAWAKRKGASKDEAMKKYVALVDALAAEMG"
     gene            11978..13180
                     /locus_tag="PLB01_00870"
     CDS             11978..13180
                     /locus_tag="PLB01_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQQ75879.1"
                     /translation="MQTLYVAAYHQSKFGKLMGLTVPEIVARAVTETAASIPADPSAF
                     DVASIGATCNFTLNEQGLLAGLVAMAPGMAAKPIEAVENACASGGEAILSVARKLLLG
                     EGETGIAVGFEKMRDAEGKMDGKLIGKVLGYFSHPDEREGKTFVFPHLFAEVMDLYMK
                     EHTVSERELAAIAVQEYANARHNPYAQMQKNDVTLEKALAIEGINRYVVDGLPLKTLD
                     CSQITDGYAGLVLATEKGLAKLGVAKKDAVRLAGFGQATDPLLKGGRDVLRPCGARTA
                     MAKAYARAGVDPRDVNVAEVHDCFTVMGAIGTEVIGKAPAGKGAAYWVDGKARPDGEC
                     GINTSGGLIAKGHPIGATGIAMVGWAAWQLLGKVPKELQVPNARAAATFNIGGPICAS
                     VCTVLTPA"
     gene            13191..13556
                     /locus_tag="PLB01_00875"
     CDS             13191..13556
                     /locus_tag="PLB01_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HQQ75880.1"
                     /translation="MAFDERLAARIRTVLRGRSDVVEKFMFGGVAFMVRGHMTCGVVG
                     STLMVRVDPEHAGAFLKEPAARPMDFTGRPMRGFLFVDAHGIATTASLRKWVGRAVAH
                     AQSRPRKLKKKPVSPRPRS"
     gene            13706..15619
                     /locus_tag="PLB01_00880"
     CDS             13706..15619
                     /locus_tag="PLB01_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HQQ75881.1"
                     /translation="MNTLWMVLIAVLVIYAAYSYARRVDRSVIQADVKRATPAKMYMD
                     GVDFMPTSRNVLYGYHFKSIAAAGPIVGPITAANIWGWGPSLLWLVIGVSLIGWASDY
                     SAIMVAVRNDGNSLSAISHKLIAPRTRRILFVFIFFYLMLLAGAFVGILAAILSARPD
                     VPFGIIMLALMGLLAGQMMYRWKMDLVLVTLIVVVATIVAMAVGPWGQRKDEKGKLVQ
                     GPVGSAIVSINASVDDLTGKQPLMQLVDPTNADPRIPPPGPDGKRPTTAAYDAATSQV
                     KTLPNYLLWMAFLFVFSYCGANMPIWRFAQPVNYIGFWIMLITIVLSAVGMIVAPLSG
                     VKDAAGNVIGAFALTAVKDLGFAAPLAGKAWQPLWPMLFVTIACGAISGWHALVGSVG
                     TARQLEYETDCLPVGAGSMLGEYSLATLSLTAVSIAGVGGGGGRFAAGVGKLIFAGTF
                     GIIPEVFGTALGFGAFVMIVLTVTQLVFRVMRVTLAEWMGDSVPLFRNMHVSSIISMA
                     LTAALVLTGTWVYLWQMFGASNQLMAALSLLVVTVWLKSEKRNPSYALYPMLFMYFTT
                     LFATCVTARNLYVTIAANPAMSGLPVAGAWAMIIVAALLLVASLVIGWDGLKAYRKYS
                     AQPPAGKPATATA"
     gene            complement(15680..16141)
                     /locus_tag="PLB01_00885"
     CDS             complement(15680..16141)
                     /locus_tag="PLB01_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HQQ75882.1"
                     /translation="MKKAVLIALLAASCSAAAAAPGDVAPARPDAVRAGNALRALDGT
                     WILRSAKFNGQPVPDRKFSSGTWTFSSGALSATNGEGTSARFTLAADPAAPDAFRIDP
                     VPPSTERAISMLFRLEGDRLTIAFFDGTSGRPVNFEPERKKMVLEFTKNGH"
     gene            complement(16155..16793)
                     /locus_tag="PLB01_00890"
     CDS             complement(16155..16793)
                     /locus_tag="PLB01_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75883.1"
                     /translation="MSQALLALVAFVLGWFALGSGANVKRGNRALKWLQGGLKALGDK
                     ATVRWIGTTAVDLGLAQAKEPFESASLVVFLAPRDLPWLWAISRARGRRDTLILRARL
                     RKHPLADVEMLDRASWSGRDALRGMKEERWSVREPSEPGGLAVFTKFDEALARADELW
                     REARTAGATVRRLAVRRNEPHLEFHVDLPADSLDSAGFFGSVRALAEIASRR"
     gene            16837..17139
                     /locus_tag="PLB01_00895"
     CDS             16837..17139
                     /locus_tag="PLB01_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75884.1"
                     /translation="MLASRPGGDVESTGWKKAAREFLYGMFLHEHVEQARDARGALES
                     LFLVVVFGDMVGLPVLPPYYALRLLPHTLGALPGWKRRILRERGLGDDHEHHLHGV"
     gene            17228..17512
                     /locus_tag="PLB01_00900"
     CDS             17228..17512
                     /locus_tag="PLB01_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75885.1"
                     /translation="MGEKRAERAGGFKGLWQGFKETIYGMSAHDMSRAALRTRASMEH
                     LFILITMGDLLGIPILPPYYSLRLLPHVVPQIATWKRRMLREKDVSDAMF"
     gene            17554..18540
                     /locus_tag="PLB01_00905"
     CDS             17554..18540
                     /locus_tag="PLB01_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HQQ75886.1"
                     /translation="MGMKEVFEQNPGRRYIMFGGKGGLGKTTFSAATAYWLASQGKKV
                     LVFSVDPQASLTDIFQKDIFGKGPVKIMENLWAQEIDADSHIKAYQDEIRKKIRDMYG
                     LAEVPEEIESYIQAASAEPAMEESAIFDAVVDVVVQKDYDYYIYDLVPLGHALYYLSM
                     AKVYDEWINKVTKLREDMREYEEMVSRLKREKETEQDEILKELVYIKGRINASSKILT
                     DKDMTAFFFVVTPEDMIIRDTEKAAKLFAQFDVPIGGYVVNRVIPHELIDQNIPDYLR
                     NRIQMQDKYIGNIKTTFGKDVLAYVPELERDVTGLPMIEKLAKIMYGDQKLG"
     gene            18552..19532
                     /locus_tag="PLB01_00910"
     CDS             18552..19532
                     /locus_tag="PLB01_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HQQ75887.1"
                     /translation="MPEIQTSMSAYIKEHAGLKYIFFGGKGGVGKTVFAAATAVHNAQ
                     LGRRTILASTNPVHSLSSLLGQNVLGKHTPVEGVPNLWAYEIETKETIERSKVEIREK
                     IQWFLKFADISTKAEDFVESATMNPAFEESAMFENMIDLMFKNEYDVYVFDTAPTANA
                     RRLLGMSKVYGLWVNKMLKSREEARTLREMLSFTKKKEQDPLMAYLISFRERMEHGRV
                     LLTDPEKTSFFFVTLPEALPIAVITRFIQWFHDFGIPVGGVLVNMLIDKKSLDAGAAD
                     FVKNRVAMQDEHMKTVWQKFDGSVRAIVPLYETEIRGVPMLKRLGDAMFA"
     gene            19550..19939
                     /locus_tag="PLB01_00915"
     CDS             19550..19939
                     /locus_tag="PLB01_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75888.1"
                     /translation="MRLGPALAGATLAISLAGCATVGRPDLSSQSCGDELARQISDVL
                     FAQGESRPISDDLANGLTGDLSTGALSPVSFFADSPSGCQYGFAIHQTSEGCVLRLYY
                     RKRPGYEVTNNITYIETRPLKGCTCSR"
     gene            19957..20535
                     /locus_tag="PLB01_00920"
     CDS             19957..20535
                     /locus_tag="PLB01_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HQQ75889.1"
                     /translation="MSLRRVLPLFVLLALAAFPAARALAATDEETIKVFRDAGESGQF
                     FDKCYGYAVFANVGKGGIGVGAAHGTGRVYEKGKVVGDVKMNQVSVGAQLGGQSYSEI
                     IFFEDKRAFDEFTSGSFEFGAEVSAVAITAGAGAKAGSSGSSAGASGGKHDAATAGGY
                     HKGIAVFTVAKGGLMYQATVSGQKFKYTPRKK"
     gene            20592..20870
                     /locus_tag="PLB01_00925"
     CDS             20592..20870
                     /locus_tag="PLB01_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75890.1"
                     /translation="MPRFLIEVPHEEAQLACARAVEVFLKSGSHYLTHADWGCKDGDH
                     RSWIVVEVDSKDAARGILPPAFRAQARIVQLNLFSMPEIDEIIRRHKP"
     gene            20956..22494
                     /locus_tag="PLB01_00930"
     CDS             20956..22494
                     /locus_tag="PLB01_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQQ75891.1"
                     /translation="MPSLHPTELTMLLDLSEALGSPLNLRASFARALEILETDLKAVF
                     GMVAFFEAETGELKVEAVTGRHDAAARKARYRLGEGISTRVVKTGRPIAVPHASQEPL
                     FRDQLGLLASRKSPKDDLSYTSVPIRIDGKSAGALAVAFVYEKNRDLGHLVKVLGIAA
                     SMLGQAARVHRLLEGERGRLMEENRQLKEKLRERYDVGNLVGTSHPMQKLFEEIAQAA
                     PANTTVLIRGESGTGKELVAHAIHYNSPRAEKPFIKVSCAALPESLIESELFGYEPGA
                     FTGAEKQKKGRFELAHGGTLFLDEVGDLPAATQVKLLRVLQEREFERLGGVKPVRVDV
                     RLIAATNIDLEEAMKEGRFREDLYYRLNVFGLFVPPLRERRSDILLLADHFVEKYAAA
                     HGKSVRRISTSAIDMLMSYHWPGNVRELENCIERAVLVCEGGVIHAHHLPPTLQTAEV
                     SNTLPGQALADAVSGFEKDLILDGLKIARGNRAKAARLLRTTERILNYKIGKLGIDAS
                     RFRP"
     gene            22836..24428
                     /locus_tag="PLB01_00935"
     CDS             22836..24428
                     /locus_tag="PLB01_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQQ75892.1"
                     /translation="MKNKKLITVLFLVGLVATSWALLAADPSGANTGGIANVPSKVAG
                     KPTVEEIGAAVGQTRVSLNFVWTLMAGFLVMFMQAGFALAETGFTRAKNAAHTMTMNF
                     MVYGIGILGFWIAGFALQMGGSGAAMGAALSVPAEMGKLIGPTIGGNVWGLFGGTGFF
                     LSGVSYDVAAFAIFLFQMVFMDTALTIPTGSMAERWKLSAFVIYGFVASALIYPIFAN
                     WAWGGGWLSTLGKYAGLGHGTVDFAGSGVVHLTGGVMAFVGAKVLGPRIGKYVKGEVR
                     AIPGHNLPMAFLGCFILAFGWFGFNAGSTLAGTDLRISVVATNTMLASASGALLAFLV
                     TWKKYGRPDPSMSANGMLAGLVAITAPCAFVNAPVAVLIGAISGVLVVYAALYIENRL
                     KIDDPVGAIAVHGVNGAWGLIALGLFADGTYGGGLNGGPEAGVAGLLYGDGRQLVAQL
                     IAIAANFVWVGLTSFVLFKVLDATVGMRVSPEQEIQGLDFNEVSAPAYAGDRPTVNTP
                     VVVSRPAAAAVAVLKPAPAGGK"
     gene            24439..24804
                     /locus_tag="PLB01_00940"
     CDS             24439..24804
                     /locus_tag="PLB01_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQQ75893.1"
                     /translation="MKLITAIIRPEKLEDVKVALFAAEVEGLTIQQVSGHGGEKAVME
                     SYRGHPLLYEFHDKVRLDIAVSEPFVQVTVKAIADAARTGQVGDGKIFVQQLDHVVRI
                     RTGEADVEALTPVTVLAKG"
     gene            24815..25987
                     /locus_tag="PLB01_00945"
     CDS             24815..25987
                     /locus_tag="PLB01_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQQ75894.1"
                     /translation="MKTPFSGGLRAALAGALFLTTGLASAADVVPPAVLPPNPPVPAP
                     TPAPAPKPWFEEISVNAFVSASYSYNFNRPDTGTNQLRVFDFDDNSFKVDVAEIVVQK
                     AVSKPGEAGFRVDFVAGSSIPRVSSSYGLLQGQDVDLQQAFVSWIAPVGSGLRLDAGK
                     FVTHLGYEVIDGYDGWNDNATRGFLFGLTLPYAHTGLKATYAFSDQLAGMLEIVNGWD
                     VARDNNTGKSIGAQVAWTPSKAVTVAGNFMTGPERANVNSDPRTTFDVVAQWKLSDLT
                     AFTVEAVYGSEKGAVKPGETASWSGVAGYARLGLSDTFAFCLRAEYISDADGARTGTA
                     QKLTEVTVTPEWKAAPHLVFRADLRVDWSNSDVFVKNDGTFKGNQATVLVNGIYVF"
     gene            complement(25994..26935)
                     /locus_tag="PLB01_00950"
     CDS             complement(25994..26935)
                     /locus_tag="PLB01_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HQQ75895.1"
                     /translation="MGGKLRVAVLGAGGVGGYFGGALARAGHPVALLARGTHLEAIRA
                     RGLEVRTPDGNFTVAVEASNDVASLGPADLAIVAVKTYSLAEVLPAARLLAKTGAAVL
                     PLLNGVEAAARLVAGGVPKECVLGGLTQISAAKVAPGVVERKSPFQNVAVGELEGGPS
                     ARGERIAEAFRDAGAQARVSLEIEADLWRKFAFIAPMAAACGLARTAIGPVREAPYGK
                     LLLERAVREVLAVARARGVALAADEEAKILAFVDGLGAGMKPSFLLDLEAGGPTELDD
                     LSGAVSRLGREVGVETPIHDTAVAALGATSRRREPPS"
     gene            complement(26958..27146)
                     /locus_tag="PLB01_00955"
     CDS             complement(26958..27146)
                     /locus_tag="PLB01_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75896.1"
                     /translation="MKRIDFAVLERVPFWLSQLVEAFGQADVRAIKVQTCDVCMEMPA
                     ARDPHSVLEEFKRRIEGR"
     gene            complement(27263..28630)
                     /locus_tag="PLB01_00960"
     CDS             complement(27263..28630)
                     /locus_tag="PLB01_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016691267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQQ75897.1"
                     /translation="MRFFEDILATVGDTPLVRLNSVTKGLKPLVLAKLESFNPGKSNK
                     DRVGIHIVEMAEKDGTLKPGGTIVEATSGNTGLGLALAAVVKGYKCICVMPDKVGQEK
                     RDLLKAHGARVVITPTSAPPGSPERYTEVAKRIAAETPGGFLANQYFNPHNRGIHYLT
                     TGPEIWRDTDGRVTCYVASLGTGGSTSGAGRYLKEQNPAVRVVGVEPVGSILGEFQRT
                     GKMVDSAPYLVEGIGQEIIPGNVEFKYIDEILTVTDRESFHLMRRLSREEGIFVGGSS
                     GTALAGALKIAERMTADDVIVVMLPDTGERYLSKFHSDGWLADHGMLDAAEMRVGDLL
                     RQKAAGDLPPLLTLKPEDPVRTALALIRKHHVSQLPVMSAAGEVVGTVVEPHLFEAVL
                     EGRATPDAAVSTAMEAPLPRLGSDEPLAAATRILTSAHALLVEEKGRPVGILTRIDVI
                     GFGSR"
     gene            complement(28636..29772)
                     /gene="moeB"
                     /locus_tag="PLB01_00965"
     CDS             complement(28636..29772)
                     /gene="moeB"
                     /locus_tag="PLB01_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HQQ75898.1"
                     /translation="MSEPFSRDEIARYSRHLLLPEVSAAGQARLRSGSVLVVGAGGLG
                     SPAALYLAAAGVGRIGLADFDAVDVSNLQRQVLYDTRDVGRPKVDVARERLSALNPHV
                     DVAVFPERLARDNVRTVFDGFDVVLDGTDNFSTRYLTNDACFLLGKPNVYGSIYRFEG
                     QVSVFQPGKGPCYRCLYPSPPEPGLVPSCAEGGVLGVLPGVVGALQATEALKLLLGIG
                     RSLAGRLLLYDALALSFREIAVPRDPACPLCGDRPSITDLVDYDALCGAGAPVLPAGA
                     EMSPADLRAWQERGEPFQLLDVREPAEAALAAIAGSVLVPLGSLPARLHEVDRSRPVV
                     VYCRSGVRSARAVALLRGAGLSRTYNLAGGILAWARDVDPGLPL"
     gene            complement(29820..30752)
                     /locus_tag="PLB01_00970"
     CDS             complement(29820..30752)
                     /locus_tag="PLB01_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75899.1"
                     /translation="MNAKVSMVAAVLGLATWSGTAPAELPGAAQKPAAPVLPAVSPEQ
                     IGVRLKALADQETKSLKGQEAGKQTARTQALRSQFQARRAVAISKAAADAAACKTTPK
                     IYEVTPDLAMPDDPVAITGCGFGESKGMVILSDGNQQLTVTSWKDNAIEASLPHLTGF
                     ADPKTVTLKVVTVEATKTAPSKAITLKPLLDLAELSPTGWVLEGCHKNFGYGIVHHTG
                     ALVSASCTDVAGTDRIRFNVALRNGWSFHSVIFTKLCGPTSGPCGGENDATPHTDVYQ
                     VGRPALPELVVDWKRTVSYYPAIRIMGPAGTPFN"
     gene            30880..31587
                     /locus_tag="PLB01_00975"
     CDS             30880..31587
                     /locus_tag="PLB01_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ75900.1"
                     /translation="MIAPFSSKTVLVAGGTGGLGRAVTLALLTEGARVSVTYRKREEL
                     VVLKAAAGAAHGRLEGYEADVTDETAARGLVEAVRARQGRLDVLVNTVGGFAGGKKLW
                     EEDASLLDRMLGLNLRSGWTLARAAVPVMLAQGRGAILNVAAQAALAPPAGLAAYAAS
                     KAAAVALFASLAAELAGSGVRVNSILPSIIDTPANRNAMPAADFSKWPKPEEIARVVL
                     FLCSDEAKLVNGAAIPV"
     gene            complement(31601..32626)
                     /locus_tag="PLB01_00980"
     CDS             complement(31601..32626)
                     /locus_tag="PLB01_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HQQ75901.1"
                     /translation="MTRRVRLPALAAAVLAAAWAVAGKPPGFADAARRKPDVTIVVTD
                     SGLGGLSVAADLAARLPASGIVKSARIVFVNAEPDPAFGYNDLRNDADKVRIFDGALS
                     AMEARWKPDLILIACNTLSVVWPRTEHAKTGGVETVEIVGLGAGLIRRELAATPDATA
                     LVFATKTTIESEAHKRLLVAQGVAPDHVVGEACPKLAGAIERGSASAETAGYVNKFVA
                     DALAKKTTKGGPLVVSLNCTHFGYVKPLWEQAFAAAGHPGVKVLDPNSLMTDVVLREG
                     GEKRFPRTAVTVEAVSKVPVDEGARANLAALLRKTSPETADALMRIRHDPALFPAAVE
                     PSAIVPR"
     gene            complement(32623..33372)
                     /locus_tag="PLB01_00985"
     CDS             complement(32623..33372)
                     /locus_tag="PLB01_00985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ75902.1"
                     /translation="MRVEQGVSGPTAADFKGLSRAETIQRLREVVDSLPLAERFRLLH
                     ELGIYHAEESLSGAGSSLAETESLRRQLPALFRRFRVTSLLDVPCGDFHWMSRLDLSG
                     VSYVGADIVPDLIESNRRRFPPPLEFRVLDVTADPLPNVDLILCRDLLIHLGTRDAAR
                     ALANVLRSGSRLLLASHYFDRKENLDIPSGDFRPINLTRAPFHLPPPAERIDEGSRLE
                     DGLYADRSMALWPLDELRRSDVPARLEELAR"
     gene            complement(33414..34364)
                     /locus_tag="PLB01_00990"
     CDS             complement(33414..34364)
                     /locus_tag="PLB01_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HQQ75903.1"
                     /translation="MADKKIIAVVGATGAQGSGLVKAILADPNGGFAARAITRDVNSE
                     KAKELAKLGAEVVAGDVDDPESLRKAFTGAYGAYCVTFFWAHFSPEKEMAHAAAMAKA
                     AKDAGVKHAIWSTLEDSRKWIPLSDTRMPTLQGKYKVPHFDAKGESNHFFTDLGVPTT
                     FLYTSFYWDNFVFFGMGPKKGPDGTLAIALPMGDKKLPGIAAEDIGACAYGIFKGGAT
                     YIGKSVGIAGEHVTGAGMAAGLSKALGREVRYAAVTPDVYRGFGFPGADDLGNMFQFN
                     RDFSDDFCAARSVEFSRKLHPGLQNFEQWLARNAKRIPLE"
     gene            complement(34385..34945)
                     /locus_tag="PLB01_00995"
     CDS             complement(34385..34945)
                     /locus_tag="PLB01_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQQ75904.1"
                     /translation="MRRSRKTAGLPGASSRPAVGTISAEIAQARPFSSLEREAAATLL
                     RTGDVLRHAVECALRPWGVSPEQYNILRILRGADANGLPTLEIAERMLARSPNITRLI
                     DKMSESGLAERRPMPDDRRVVRVVATARGRKTLAELDEAIEATLAKLAPLPPSRLKTL
                     IKLLDDVRGRLAIRTAREAAAIPQQR"
     gene            35053..36369
                     /locus_tag="PLB01_01000"
     CDS             35053..36369
                     /locus_tag="PLB01_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75905.1"
                     /translation="MTKRPVFRCKLRNAFALSVLIVLAAGSAGVSRAQAPPPAPALKP
                     VRPGKAPANPYTRKQIARGKLLVVSSGCNDCHTPWIVDPELGVPAPDWTRMLSGHPEG
                     GPDPQGEIGPADIGLIGPTFTSFKLPFGITYSMNLTPDIDTGTGSWTEKMFLDVFRKG
                     RHLGGDGRPVYPPMPWDSYRSLSDEDLRAIFAYLRSIPPLRNLPPTEKVPPPVEGAIV
                     ELNNKVIALQANPNAKLEAPKNGPPPPPPLALKPVVKGKAPKRTYSADMVRKGKLIVT
                     AGACNHCHTPWVYNPVLGAPAPDWTRMLSGHPEGAPDPQGKPGPQDIALIGPTFTSFQ
                     LAFGTVYSKNLTPDIETGSGAWTEEKFLKIFRTARHPDGGVLLPPMPWNMIRILPDSD
                     LRAVFAYLQSIPPLRNEVPMSKVPPEAQKMIEGVNAKMLLHPAGPY"
     gene            complement(36370..36867)
                     /locus_tag="PLB01_01005"
     CDS             complement(36370..36867)
                     /locus_tag="PLB01_01005"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ75906.1"
                     /translation="MASKKKSKKPAAKSSPKKAKSAKKAPAKRRAPAAKPGWKLESVA
                     PGITANDLEKSLGWYRDVVGFAVEETWTHEGKVAGAQLRAGNVVVLLGQDDWKKGRDR
                     KKGEGFRLYWMATGDIDAMAKGIVARGGTLDQPPTDQPWGMRDFSLTDPDGFKITVAK
                     VTKKR"
     gene            complement(36893..37663)
                     /locus_tag="PLB01_01010"
     CDS             complement(36893..37663)
                     /locus_tag="PLB01_01010"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ75907.1"
                     /translation="MAEVTSHAPGAFCWIELGTTDQKAAKDFYTKLFGWSFTDNDMGP
                     GMTYTIFHLNGRDAAACYTLEAAKMPGVPPHWMLYVATADADASARRAGELGGKVNAP
                     AFDVMDFGRMAVLADPTGGNFCVWQAKANRGIGVQDEPGAFCWGQLNTNETAKAEAFY
                     TALFGWRAKTGTGGGMTYTEWVLGGKPIGGMMAPPDGARVPAHWLPYFAVADCDAAAA
                     RAVSLGARTYVPPASIEGTGRFAVLADPQGAVFAIYRA"
     gene            complement(37734..38147)
                     /locus_tag="PLB01_01015"
     CDS             complement(37734..38147)
                     /locus_tag="PLB01_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQQ75908.1"
                     /translation="MKAPGPFSKWSSRAPQLQSILRIVAAALFILPGTMKLFAFPIGV
                     PPAGGTVPLLSQLGLGAVLEVVGGSLLVLGLFTRPVAFLLAGEMAVAYFQFHAPKGFW
                     PTVNGGQLAILFCFVWLYFSAAGAGPWSLDAARGK"
     gene            complement(38144..39574)
                     /locus_tag="PLB01_01020"
     CDS             complement(38144..39574)
                     /locus_tag="PLB01_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQQ75909.1"
                     /translation="MPARAAVREAVPEAREVVISRLFDAPRALVWEAWTSHAQLARWW
                     GPMGFTTPRGSVELRPGGPFTLVMRAPDGTEYPLEGFVREAEAPARLVFSALDHDEIR
                     IETVVTFEEEGGKTRLTVRQTVPSNPMMARGQKPGWSQSLERLATALVESNEAGASSS
                     PTADREIATSRVFDAPRELVWRAWTDPQHLARWYGPNGFAVTTDEMDVRAGGVWRHTL
                     HGPDGTDYPNLTRYVEVVRPERLVYEHGSAERDEDRFDVTVTFTDEGAGKTRVSTRMV
                     FATRERRDRMVNVFGALQGQRQNLDRLEAHLASLGDGPKLVLTRTLDAPRDLVWKAWT
                     EPARLSAWWGPKGFAWARGTMDLRPGGIFHYGMTAPDGKTTMWGRFVYREVVEPSRLA
                     FVVSFSDENGGVTRHPMAADWPLEILNVLTLEASGGRTTLTLEGWPLHASESERRAFE
                     GGIGSMEKGFAGTFGQLVAYLATAVA"
     gene            complement(39549..39938)
                     /locus_tag="PLB01_01025"
     CDS             complement(39549..39938)
                     /locus_tag="PLB01_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ75910.1"
                     /translation="MTPAPDALSSTFSALADPTRRAILARLASGEAGVTELAEPFDMS
                     LPAVSKHLKVLERAGLIARGREAQWRPCRLEAGRLRDVADWVEHYRRFWEESFDRLDD
                     YLRELKAQEAKNAAKKESRHARKSRRA"
BASE COUNT         6112 a        14006 c        13639 g         6338 t
ORIGIN      
        1 gactttgatc acttcggagg tggaatcatg ggttggatca tcactttgat catcggcgga
       61 atcatcggct ggctggcgag catcgtcatg aagacgaacg cccagatggg cctgatcgcc
      121 aacgtggtcg tcggcgtcgt gggggccggt ctcggcggct ggctcgccgg cgtcctcggg
      181 atcggggcca cgggcagcgt gatgagctgg atcgtcggga tcgcgggcgc cgcgatcctg
      241 atctggatcc tcaaggcgct cggcgtcttc aagtagacgg acggcgccac cgggcccgcc
      301 gcatgcgagc ccggcacctg cggagacacg cgccgatcag accgggacgc ggaagctctg
      361 caggaccctc atgtagttcg cgcgctcgaa cgccgacggg tctgcgctcg tgcggtggct
      421 gacggcgccc gtcatctgcg cgacggactc gtactcgtgc ttctccatcc actcggcgag
      481 cccctgcttg agcgccgtga tctgccgcgg gccgtgccgg aggatcgagg acacgatctg
      541 aacggcgtgg gcgcccgcca tgatcgcctt gacgaggtcg atcggcgtgt ggacgccgcc
      601 cgtctccgcg agcgagcacc gcacgcgccc ggacaggatc gcgagccagc ggagcctcag
      661 gaggaggtcc gaggagtccg agagctgaag ggccgcgacg ggctcgagga gctcgacgtc
      721 gatgtcgggc tggtagaacc ggttgaagag gacgaggccg tccgccccga ggtcgtcgag
      781 ctggcgggcc acgttcgcga acgacgtgaa gtacggcggg agcttcacgg ccacgggaat
      841 cccgaggccg cgcttgacgc tgcggaggat gttctcgacc tcgtgctcga tcgtcgtccc
      901 cggcacgttc ggatccgccg cgatgcggta gacgttcagc tcgagggcgt gcgcgccggc
      961 atcctgcatg agcttcgcgt agctcagcca gccggaatcc gtcatgccgt tgagcgaggc
     1021 gatgacgggg atcgagacgg tcgcgcggat ctttcggatt tgctcgaggt accggtccgg
     1081 cccgaggtgg aactcctccg gcgccgggaa atacgaaagg gcttccgcaa acgactcggc
     1141 gtggacgtcc atcgcgcggt ggatgccctc gcgctcgtgc gtgatctgct cctcgaacag
     1201 cgagtgcatg acgatcgccg acgcgcccgc gtcctcgagg cgcttcaccg tgtcgaggtc
     1261 gtcgacgagc ggcgaggcgc ccggcatgag cgggtgggcg agcggaaggc cgaggtaggt
     1321 cgttctcagg tccatgtgcg gcgtcctcag ttcccgggcc gggtcacggc ctcggcgacg
     1381 tgcttcttgg cgagctgctc gtagaggaga accttgtcgg cggcctcgcg gcgcgcccgc
     1441 gcgaccacgc ccttgtagag ctccgggtcg cgctcctcgg ccacgcggaa gcgcgcctcg
     1501 ttgcgcatga aggccccgat gtcgcccttc agcgtgcccg tgtcgatcac gagcgggatc
     1561 tcccccgccg ccgtgcggcg cgggtcgaag cgatagaggg gccagtagcc ggactgcacg
     1621 gccttcgcct gctggtccag ctggaaggcc atgtcgtagc cgtgcgcgat gcacgggctg
     1681 tacgcgagga tgatcgaggt cccggggtag gactcggctt cctggaatgc cttgacggtc
     1741 tgggcgtcct tcgcgccgaa cgcgacgcgc gcgacgtaga ccgacccgta gcccatcgcc
     1801 atgaggccga ggtctttctt cgagacggcc ttgccggccg tcgcgaactt cgccgaggcg
     1861 ccgaggggcg tcgacttgga cgcctgtcct cccgtgttcg agtagacctc cgtgtcgagg
     1921 acgagaatgt tcacgtcgcg cccgacggcc atcacgtggt cgaggccgcc gaacccgatg
     1981 tcgtaagccc agccgtcgcc gccgacgatc cagatgctct tcttgacgag gtagtccgcg
     2041 agggacgcga ggcgcttcgc ctcgggcctc gggcttgccg caaggcgctt gcgaagctcg
     2101 gcgacgcgcg cgcgctgcgc cgcgatgccg gcctcgcccg actggtcggc gccgaggatc
     2161 tcgtcgacga gctgctgccc gatctccgcg gcgtgggcct tgagggccgt tcgcgccgtg
     2221 aggtcgtggt ggtcgacgcc gaggcgcatc ccgaggccga actcggcgtt gtcctcgaag
     2281 agcgagttcg accacgtcgg accgcgcccg tccctgttga cggtgtacgg cgtcgtcggg
     2341 aggttcccgc cgtagatcga cgagcagccg gtcgcgttcg cgatcatcgt ccggtcgccg
     2401 aagagctgcg tcaggagctt gatgtacggc gtctcgccgc agccggcgca ggcgcccgag
     2461 tactcgaaga gcggctccag gaactgcgcg gacttcacgt ccgcgatgcg gagtcgcgtg
     2521 cggtcggctt cgggcaggtc gaggaagaac tcgtagttgg cgcgctccgg ctcgcggagc
     2581 ggggcctgcg gcaccatgtc gagcgccttg tgcttcgggt tcgacttgtc cttggccggg
     2641 cagaccatca tgcagagcgt gcagcccgtg cagtcctccg gcgcgacctg gatcgtgtac
     2701 ttgtggccct gccactctgt cgccttgaag tcgacggact tgaacgtcgc cggggcgccc
     2761 gcgagggccg ccggctcgta gagcttggcg cggatcgccg cgtgagggca gaccatcgcg
     2821 cacttgttgc actggatgca gagcttctcg tcccacaccg ggatctcgag cgcgatgttc
     2881 cgcttctccc acttcgcggt gcccgtcggc cacgtgccgt ccggcgggaa cgcgctgacc
     2941 gggaggaggt cgcccttgcc cgcgagcagg acgccagaga cgcgctggac gaagtccggg
     3001 gcttccgtcg agacgggcgg cggcatgtgc cgctgcgtcg tgaccgaggc gggcaccttc
     3061 acttccttga ggcccgcggc cgcgccgtcc acggcggcga agttcttctg gaggacgtcc
     3121 tcgccgcgcc gggcgtagct cttgcggatg cccttcttga tgcgctcgag cgcgtcgtcc
     3181 ggaggcagga cgccggagag cttgaagaag caggcctgca tgatcgtgtt gatgcggccg
     3241 cccagcccga tctccttcgc gaggccgtac gcgtcgacga cgaaaaagcg gagcttcttc
     3301 ttgacgatct gctcctggac ctcgaacggg agctggtccc agacctcgtc gaccccgtac
     3361 ggcgagttga ggaggaacac gccgccgctc ttgacgcaag acagcatgtc ctgcttgtcg
     3421 agcagcgaga actggtggca tgcgacgagg tccgcgttcg agacgagcca ggcggcccgg
     3481 atcggcgcct cgccgaagcg aaggtgcgac gtcgtgacgg cgcccgcctt cttcgagtcg
     3541 tagacgaagt aggcctgcgc ctccttgccc gcgtcctcgc cgatgatctg gatcgagttc
     3601 ttgttcgcgc tgacggtgcc gtccgagccg aggccgaaga acacggcctt cttgcggtcg
     3661 gcgggctcga tgtcgatgga gtcgtcccac gggagcgacg tgtgcgtcac gtcgtcgacg
     3721 atgccgatcg tgaactgccg cttcggcgcg gccttcgcca tctcggcgta caccgacgcg
     3781 accattgcgg gcgtgaactc cttcgaggag aggccgtacc gccctgcgac gacgcgcggc
     3841 tcggaggcga acttagagag accggtcgtg cgggcctcgg cgagcgccgc gaccacgtcg
     3901 agccagagcg ggtcgccggg ggcgccgggc tccttcgtgc ggtcgaggac ggcgatcgtc
     3961 ttgaccgtcg cgggcagcgc cgcgacgaag tgcgcgaggg accacggacg gtagaggcga
     4021 accttcagga cgcccacctt ctcgcccttc gcgttcagcc atttcgcggt ctcgtcgcag
     4081 gtttcggctc ccgagcccat cacgacgatc acgcgctcgg cgtctttggc cccgacgtag
     4141 tcgaagaggt tgtacgcgcg gcccgtgagc tcggtgaact tcgccatcgc gtcgaccacg
     4201 aagcccgcgc acgcgtcgta gaacgggttg caggcctcgc gcgcctggaa gaacgcgtcg
     4261 gggttctggg cggtgccgcg caggatcggc cggtccgggc tgagggcgcg ggcgcggtgc
     4321 gcgtcgatct tcgactcgtc gacgagggcg cgcaggtcgt cgtccgtgag ctcctcgatc
     4381 ttcgcaagct cgtgcgacgt gcggaacccg tcgaagaaat gcaggaacgg gatccgcgaa
     4441 gcgagcgtcg cgccgtgcgc gatggccgcg agatcctgcg cctcctggac ggaagccgag
     4501 gccaggaggg cgaaacccgt ctggcggcag gccatgacgt ccgagtggtc tccgaagatg
     4561 gacaacgcat gcgtcgcgag cgagcgcgcg gacacgtgca tcgcgtagga ggtgagctcc
     4621 cccgcgatct tgtacatgtt cgggatcatg aggaggaggc cctgcgaggc cgtgaacgtc
     4681 gtcgtgagcg cgccggcctg cagcgagcca tggaccgcac cggcggcgcc gccctcggac
     4741 tgcatctcga tgacctgcgg aatcgtcccc cagacgttct tcaggccctt ggccatccac
     4801 tcgtcggaga gctcgcccat cgtggaggaa ggagtgatcg gatagacggc caccacctcg
     4861 ctcaggcggt aggccacgga ggcggcggcg gtgttgccgt cacagacgag tcggcgtcga
     4921 tttcccatgc gtcggagctt agagacggag tctctccgga gacatgatgc aaacggacca
     4981 aagctccgca ttccgtcgcc ggactggggc atccggctcc cttgcgggag gcgggggcgg
     5041 gtcctagctt gttctcagga ccggctttcc ccggccccgg agggtgccat gcagaagagt
     5101 catttcaggc tctgggcgct cctgggcggc ggagtcatcc tcgtgctcgc cctgggaggc
     5161 ctctggaagg tccagacggc gcccgtcgac acgacgctga cccccgcgcc cttcgcgctc
     5221 ccgctgtcca agacctcggc cgatctccag gtcctcgtcg cgggggtccc gctcgagaag
     5281 gcgatcgcag acaagcgcct gatgctcgcc ggagagaacg gcgcggcggc gatcgtcgtg
     5341 cccgaggcga cggtccggct caacgaggag gaccacgcgc gcctccggcg cgtgccgtcc
     5401 ctgctcgctc tcgccgcgat ggcgggcggc gggctcgtcc tcttcctgat cgggctgctc
     5461 acgccccgca tcggggcctt caagcagcac ggcctgatcg acatgcacct cgagtccatg
     5521 taggccccgc cgggcccgcc ggactcagac catccggaga agggacgcct tgcgccgctc
     5581 gtagggcgtc ccgaggccga gcccgagcgc cacgtcgcag agccgaaccg cctcctcgaa
     5641 ctcggcgcgg tcgctccgca gggcgatccc gcgctcgagg aacctgagct cggcgagttc
     5701 ccgcgtgaag cgccgcccga ggtcctcgcg ggcgacgtcg agatagccct ccgcctcggc
     5761 gagcgccgcg agcgcttcgt cctcgtccgg ggcgtccgcg gcgaggagag cccacgcgaa
     5821 gaacttcacg ccgttcggaa gggagtccga ctgctcgatc gcgcggcgcg cgtacttcgc
     5881 gccctcgttc cgcagggtcg cctcgcggtg gcccgcgagg aggtcgtcca gccgctcctc
     5941 ggggctcttg cggcagggtt cggtgcgccg ggccatgacg ggccggattc tagaggcctt
     6001 atcctcccgc cgtgccgaag aaggccgtcc tcgccctcgg gctcctgctc gttctcgcgg
     6061 cggtcgcggc gtacgtcccc gcgctgaagg ccgggttcgt gtggaacgac gacacgtacg
     6121 tcacggagaa cccgacgctg gacggcctcg ccggcctccg cctcatctgg accgacacga
     6181 aggcgaacga gcagtactac ccgatggtct tcacgtcctt ctgggtcgag aagcgcctct
     6241 ggggcctcaa gcccttcggc tatcacctcg tgaacgtcct cctccacgcg gggagtgcgc
     6301 tgctcctgtg ggcgctcctc gcgcggctcg gcctcaaggg cggcgcctgg gctgcggcgc
     6361 tcttcgccct tcacccggtg tgcgtcgagt ccgtcgcgtg ggtcacggag cggaagaaca
     6421 cgctttccct cttcctctcc cttctcgcga tgctcgcgta tctcgcgggc cggagggaga
     6481 aggaaaggga caaagagaag aaggaagatt ttcttagaaa gggtaagagg gggcgggcgg
     6541 agcagcgctc gcccgcggcg cagcgttcac ccgcggcgca gccctctcgc ggcgcgcggc
     6601 atccccttct ttcattcata ggattcttcc tctttatcct cgccctcttc gccaagacga
     6661 cggccgtcgt cgttccggcc gtcctcctcg tcctcgtctg gtggaagcgc ggggagatcc
     6721 gcggcaagga cgtcgggccg ctcgttccgt ggttcgcggc cgggatcgct ctcgccgccc
     6781 acacggcatg gctcgagcgc accgtcgtgg ccgccacggg ctcggagtgg tcgctcggcc
     6841 tcctcgggcg cgtcgtcctc gccgggcgca cggttctctt ctattcgcag aaatttctgc
     6901 ttcctatcga tctctccttc ttctacgagc gctggaaggt cgacgcgagc gcccccctcc
     6961 agtggctgcc gacactcgcc gcgctcgccc tcctcgtcct cgcgtggcgt ttccgggagc
     7021 gcctaggccg tggccctctc gcgttcctgc tccttttcgg cggcgtcctc ttccccgcga
     7081 tgggcttctt caacgtctac gcgatgaggt actcctgggt cgcggaccat ttcgcctacc
     7141 aggccgtcgc ggccgcgtcc gcgtgcctcg cctgcggcgc ggtgacgctc cttcagaggg
     7201 cgccggtcgc cgcgcggcgc gcagcggctg cggccgccgc cggaatcctc gttctcctcg
     7261 gagtcctcac ctcgcgccag gcccgcatct acgaaggaga ggaaaccctc tggaaagaca
     7321 ccctttcaaa gaatccttcg tgcttctcct gcgaaacgaa ctacgggttc tttctcgtga
     7381 acgcggggcg cacgtccgag gcggtcgccc acttcgagag gtcgctgaag ctgaagcccg
     7441 acaacgtccc ggccctcctg aacctcgggc gcgccgccga gcagcgcggg agcctcggcg
     7501 aggccgccac gcgcctcagg gccgcgtacg cgatcgaccc gcgcgacccg gccgtcctcg
     7561 tcaacctcgc gacggtcgag gtcaagctcg ggcgggccgc cgaggcgatt cccctctacg
     7621 aggaagcgct gcgcctcggg accgccgacg cgcacctcgc gcacaacggg ctcggcgtcg
     7681 cgttcatgcg gcagggccgg gtggccgagg ccgcggagca gttccgcgaa gctcttcgcc
     7741 tgaggccgga ctacgacttc gcgcgcgcga acctcgggcg ggccctcgcc gccctcggcg
     7801 cggctccctg acgcttactt cgatccctgg agctgagcga ggtcggcgag cacgtcgtcg
     7861 ctgtgcgtct gcgggttgac cttcagatag accttccgga tcacgcccgc cgggtcgatc
     7921 aggaacgtgt tgcgggcgga gtacgtcttg ccgtcgcgga ccacggccga gtcgtagacc
     7981 ttcgtcgcgg cgccttccgt gtcggcgagc agcttgaagc tcagcccttc cttcgtgcag
     8041 aagcccttgt gcgagtccac gctgtcgacg ctgacgccga ggatgacggc gttcgccttc
     8101 tcgtactttg cgagatcgcg ctggaaattg tgggcctcca tcgtgcagcc cgacgtgaag
     8161 tccttcgggt agaagtagag gacgacccac ttgcccttgt tctgctcgag ggtcaccgtc
     8221 tgcccctcct ggctcggaag ggacacggcc ggggccttcg tgccgacggc cgggggctcg
     8281 gcggcggcgg ggcgcggggc ggcaaacacg agggcagcgg ccgcgatgga gcggaggaga
     8341 atcttcatgc cgaagagcct agcagaccga gtcccagcga gatcgcgtca tttgcccggg
     8401 tcgtccgccg aggggccgta gatcgacggc accttcgagc ccatcggccg cagatacgtc
     8461 gagagctgcc cgcgatggtg gatcgcgtcg aagaggaagc cccacatcat gtcgccgagc
     8521 ggggccttcc aggccacctt tccatccatc agaaagcgtg ccggcttctt ccaggccgcg
     8581 tccttcacct tcgcaaggcg acgcttcacg gctgccgcgt tcttctcgta cgcggcgacg
     8641 gcctccgcga cggtcgcggg agcggggccc atcgcaaagc tcacctcgcc cttgtcgagg
     8701 acctcgcagg cgtcgtggag ctcgctcgcg agcatccaga cgagctcgcc ggccgacatc
     8761 gacctcgggt gaggccggta gccggcgccc gactcgggca cggccttcag gacccggacg
     8821 aatttcggga cctccgactt gaagcactgg acgaagtact cgcgcgtgtt catcgtttcc
     8881 tcccttgcgt ttgtgacctg agtttcgccg ccttgcggat ccagccgcgg agcttcgcgt
     8941 ccgacggcag gtccgacagt ctctcgatcc ggccgagcga ccccatcccc tcgcccggct
     9001 tcgcggaggc gccgaggacg gcccggtcca gaccccgcca gaacccgaac cccgcgtgcg
     9061 ccttgaatgc ggccatgtag cagaggatcc ctccgtcgac cgtgtagaac ggcattcccc
     9121 acttcacggt ttcgtccccc tcggggcacg ccgcgtggac ggccgcccgg agccttcgca
     9181 ggatcggccg ggcgaacggc gcggccttcg cgaggtaggc gtcgacgcgc tcgtccttct
     9241 tcacttcgcc tccgcgagcg ccttcaggcg agccgcctcg aactcgtcgc ccttgttcca
     9301 gcgcggcatc tccgccgcgt cggcggcgcg gacgccgagc cagaacgcga ggcgcatgtc
     9361 ctcgaccgcg cccgagaagt cccagctctc cttgaactcg tccgacggct ggtggtagtc
     9421 gttcttctcg tacgcctcga ccatcgcctt gccgaggccc ggcctgccgg tcacgtcggt
     9481 gcccttcttg acgtacgcgg cgggcacgcc cgccttcgcg aagttgaact gatccgagcg
     9541 gtagaagctg cccttctccg ggaactcgtc accgtgcacg gtgcgcccct gcatcctcgc
     9601 gagcgccgtg aagtccgtgt cgagcgacga tttcccgagg ccgatcatcc cgacgtccga
     9661 cgtcttcccg tagaaaccga tcccgtccac gttgatgttt gcggcgatcc gcccggccgg
     9721 gaccgggggg tgcagcgcga gccactcgga gccgaggagc ccctgctcct cgcccgcgac
     9781 gagcgcgaag tagacgctcc gggcgggtct ctccttcaac tccgcgaacg cctgcgcgat
     9841 cgcgaggacc cccgcgacgc ccgacgcgtt gtcgagggcc ccgttgtaga tcccgtcgcc
     9901 gcccggagcg gccgcaacct tcacgccgag gtggtcgtgg tgcgccgtgt agatcacggc
     9961 ctctccggcc cgcttcggat cggagccgcg gaggacgccg accacgttgc cggactcctt
    10021 ccgctgcacg gtgctcgtga acgcgagcga catcgtgacg cccagcggca ccggcttgaa
    10081 cgcacgcgtc tccgcggcgg cccgaagcgc gtcgaggtcc tttccgccga gcgcggcgag
    10141 cttcttcgac agctcgtccg tcgcccacat cttcatcgcg acgcgcggcg agccgtcgtc
    10201 gggcagctcg aagagctcgc ccttccacga cgtctggatg acctgccagc cgtagcccgc
    10261 cgacggggtc gtgtggatga cgatcgcgcc ggccgcgccg tgcttcgccg ccatgaggta
    10321 cttgtagtcc catcgcccgt accagaggcg cgtcttcccc gcgaagaggt tcggctcggc
    10381 cggcgtcccc tccgggtcgt tgttcatcat caggaggacc ttgcctttca ggtcggctcc
    10441 cttgtagtcg tcccacgcgt actcgggcgc gacgatcccg tacccgacga agacgatctc
    10501 ggcgttctcg atcttcgagg cggccttctg gtcgcccgcc acggccacga aggtctcgcc
    10561 gagaacgccg gtggcggtcc ccttcggcga cgagaatgcc gcgggcccgg cgtacgcggg
    10621 ccggatgccg acgagcggga ccttctggat ccaccccccg ccgggcgcgc cgggctcgag
    10681 gccgaggctc tcgagccggg cctggatgta cgcctccgcg agcgcgtcgc cgcgcgacgc
    10741 gggtccccgg ccttcgagga ggtcggacga gaggaaccgg atgtcggctt tcaggccttc
    10801 cgcggtgatt ctcccggcgg cttccctctg cgccggcgtc acgaggtcgg ccgcgaagag
    10861 gctgtgcccg gcgagggcgg cggcgaagag gacgagacgg tttctcatga cggggacgct
    10921 aacaagccgc cccggccggc gcaacgccct acccgtcgcg ctccctgaag tcgtcccatt
    10981 cctgctcggt ccaccactcg cgctcgaggc cttcgcacga gagcccgtag gacgggcaga
    11041 tcacgcagcc ctcgccgcgg atcgcgcccg cgtcctcgct gggcgggtag agggcgagga
    11101 gccgctcggc cccttcccgc gcggcgctct cctcggcgtc gggcgggcgg cccccgaggt
    11161 ccggcgctcc cgcgcgccgc aggcgggcct cgctctcggg caggaggacg gagttctcgg
    11221 cgtcgatgtg ggcgaggaga tcccgccggt agcgtgtcga gaggtcgaga agacgccccc
    11281 cctgcccttc cggcctcgcg agcagcgggg cgaactcctc gagcgttttc gccatggcgg
    11341 cgtgctggtc gaagagcgag cgcaccggcc cggagtcggg gcgaatgggc aggtgcgcga
    11401 ggagcgccgg gaagagcgtg tcctcctcgc gggcgtgatg gtagcgcccg gcgaagagcc
    11461 ggaagaacgc gaggaacgag gccgcgtccg ccggatctcc ccgccccccg aggcgcgcct
    11521 cgacgtacgt gcgcagcgcg ccgagcgtcc gctcgatcac gacgtgctcg gcgcgcaggt
    11581 cctcgatcag cctcaaggag cggtcagccc atctcggcgg cgagcgcgtc cacgagcgcg
    11641 acgtacttct tcatggcctc gtccttcgag gcgcccttcc gcttcgccca cgcgtcgtac
    11701 tttgcgcgtc ccttcaggtc gagaaggccg ggccgcgtgc ccgacacgtc gccttcggtg
    11761 gcctgtttgt agaggccgta gaggtcgagg agctggtcgt tggacgggcg ggacgggagt
    11821 ttctcgaccc gcgccttggc gtccttgaac tggtcggcga cggatttcgc cttcttggcc
    11881 ggcatgcgtt gtcctccgtg cgcgcgattc tgcggcgaag cccggcgatc ctgcaaacgt
    11941 tgccgatccc ggcagagggc attagattcg caggatcatg cagactctct acgtcgccgc
    12001 ctaccaccag tcgaagttcg gaaagctcat ggggttgacg gtccccgaga tcgtcgcgcg
    12061 cgccgtgacg gagacagccg cctcgattcc cgccgacccc tcggctttcg acgtcgcctc
    12121 gatcggggcc acgtgcaatt tcaccctcaa cgagcagggc ctcctcgccg ggctcgtcgc
    12181 gatggctccc ggcatggcgg ccaagccgat cgaggcggtc gagaacgcct gcgcctcggg
    12241 cggcgaggcg atcctttcgg tcgcgcgcaa gctcctcctc ggcgaaggcg agacgggcat
    12301 cgccgtcggc ttcgagaaga tgcgcgacgc cgaggggaag atggacggaa agctcatcgg
    12361 caaggtcctc gggtacttct cgcaccccga cgagcgcgag ggaaagacgt tcgtcttccc
    12421 gcacctcttc gccgaggtga tggacctcta catgaaggag cacacggtct ccgagaggga
    12481 gctcgcggcc atcgccgtcc aggagtacgc gaacgcgcgg cacaacccgt acgcgcagat
    12541 gcagaagaac gacgtcacgc tcgagaaggc gctcgcgatc gagggcatca accgctacgt
    12601 cgtggacggc ctccccctca agacgctcga ctgctcgcag atcaccgacg gctacgctgg
    12661 gctcgtcctc gcgaccgaga agggtctcgc gaagctcggc gtggcgaaga aggacgccgt
    12721 gaggctcgcc gggttcgggc aggcgacgga cccgctcctg aagggagggc gggacgtcct
    12781 ccgcccgtgc ggcgcgagga ccgcgatggc gaaggcgtac gcgcgcgccg gcgtcgaccc
    12841 cagggacgtg aacgtcgcag aggtccacga ctgcttcacc gtgatgggcg cgatcgggac
    12901 cgaggtgatc ggcaaggcgc ccgcgggcaa gggggcggcc tactgggtcg acggcaaggc
    12961 gcggcccgac ggcgagtgcg ggatcaacac gtcgggaggc ctcatcgcga agggccatcc
    13021 catcggggcg accggcatcg ccatggtcgg ctgggccgcc tggcagctcc tcgggaaagt
    13081 ccccaaagag ctgcaggtac cgaacgcccg cgccgcggcc acgttcaaca tcggcgggcc
    13141 catctgcgcg tccgtctgca cggtgctgac gccggcgtga cggggtaaga atggcgtttg
    13201 acgagaggct ggcggcgcgc attcgcaccg ttctgagagg tcggagcgac gtcgtcgaga
    13261 agttcatgtt cgggggtgtg gccttcatgg tcagaggcca catgacctgc ggcgtcgtcg
    13321 gttccacctt gatggtgcgc gtcgacccgg agcacgccgg ggccttcctg aaagagccgg
    13381 ccgcgcgtcc gatggatttc accggacgtc cgatgcgtgg attcctcttc gtcgatgcgc
    13441 atgggattgc cacgacggcg tccctgcgga agtgggtggg gcgggcggtc gcccacgcgc
    13501 aatccaggcc caggaagctc aagaagaagc cggtgagccc tcgtcccagg agttagctgg
    13561 cgctcgcagc tccgccgggc gcaagtgact cgcttcccgt cacttcgccc ccgcatttgc
    13621 atcatggccg gcgggggcac ccggacctag gtttcggcca aatcgggggg aatctcgtcc
    13681 aaagaggccg aaaggagccc gtttgatgaa caccttgtgg atggtcctga tcgccgtcct
    13741 cgtcatctac gcggcctaca gctacgccag gcgcgtggac cgaagcgtca tccaggcgga
    13801 cgtcaagagg gcgactccgg caaagatgta catggacggg gtggacttca tgcccacgtc
    13861 ccggaacgtc ctgtacggct accacttcaa gtcgatcgcg gccgcaggac ccatcgtcgg
    13921 cccgatcacg gcggcgaaca tctggggctg ggggccgtcc ctcctctggc tcgtcatcgg
    13981 cgtgagcctg atcggctggg cgagcgacta ctccgcgatc atggtcgccg tccggaacga
    14041 cggcaacagc ctgagcgcga tctcgcacaa gctcatcgcc ccgcgcacgc gccggatcct
    14101 gttcgtcttc atcttcttct acctcatgct cctcgccggc gcgttcgtcg gcatcctcgc
    14161 ggccatcctc tcggcgcggc cggacgtccc gttcggaatc atcatgctcg cgctcatggg
    14221 ccttctcgcg ggccagatga tgtaccgctg gaagatggac ctcgtcctcg tgacgctgat
    14281 cgtcgtcgtc gcgacgatcg tcgcgatggc ggtcggcccg tggggccagc gcaaggacga
    14341 gaagggcaag ctcgtgcagg gaccggtcgg cagcgcgatc gtgtcgatca acgcctccgt
    14401 cgacgacctc acgggcaagc agccgctcat gcagctcgtg gatccgacga acgccgaccc
    14461 gcgcatcccg ccgccgggcc ccgacggcaa gcggcccacg accgccgcgt acgacgccgc
    14521 cacgtcccag gtgaagacgc ttccgaacta cctcctctgg atggcgttcc tcttcgtctt
    14581 ctcgtactgc ggcgcgaaca tgcccatctg gcgtttcgcg caacccgtca actacatcgg
    14641 cttctggatc atgctcatca cgatcgtcct ttcggccgtc ggcatgatcg tcgcgccgct
    14701 cagcggcgtg aaggacgccg ccggaaacgt gatcggcgcg ttcgcactca cggccgtcaa
    14761 ggatctcggc ttcgcggcgc cgctcgccgg caaggcctgg cagccgctgt ggcccatgct
    14821 gttcgtgacg atcgcctgcg gggccatctc gggctggcac gcgctcgtcg gctccgtcgg
    14881 caccgcccgg cagctcgagt acgagaccga ctgcctgccc gtcggcgccg gctcgatgct
    14941 cggcgagtac tccctcgcca ccctttccct cacggccgtc tccatcgcgg gagtcggcgg
    15001 cggcggcggc cggttcgcgg cgggcgtcgg caagctcatc ttcgccggga ccttcgggat
    15061 catccccgag gtcttcggca ccgcgctcgg cttcggcgcg ttcgtcatga tcgtcctcac
    15121 cgtgacccag ctcgtcttcc gcgtcatgcg cgtgacgctg gccgagtgga tgggcgacag
    15181 cgtgccgctg ttccgcaaca tgcacgtgtc gagcatcatc tcgatggcgc tcaccgcggc
    15241 gctcgtcctc acgggcacgt gggtctatct ctggcagatg ttcggcgcct cgaaccagct
    15301 catggcggcg ctctcgctcc tcgtcgtcac ggtctggctg aagtccgaga agcgcaaccc
    15361 gagctacgcg ctctacccga tgctgttcat gtacttcacg acgctcttcg cgacgtgcgt
    15421 gacggcccgg aacctgtacg tcacgatcgc cgccaacccc gcgatgagcg ggctgcccgt
    15481 cgcgggcgcc tgggcgatga tcatcgtcgc ggcgctgctc ctcgtcgcgt cgctcgtcat
    15541 cgggtgggac ggcctcaagg cgtaccgcaa gtactccgcg cagccgcccg ccggcaagcc
    15601 ggccacagca acggcctgag gccggccctt cccgcggttt tcctcgccgc cgcgacaccc
    15661 gtcgcggcgg cgtttccgtt cagtgcccgt tcttcgtgaa ctcgaggacc atcttcttcc
    15721 gctccggctc gaagttcacg ggacggccgg aggtgccgtc gaagaaggcg atggtcagcc
    15781 ggtcgccttc cagccgaaag agcatcgaga tcgctcgctc ggtcgaaggc gggaccggat
    15841 cgatgcggaa cgcgtcaggt gcagcggggt ccgccgccag cgtgaaacgg gccgacgtcc
    15901 cttctccgtt cgtggcggaa agggcgccgc tcgagaacgt ccacgtcccg ctcgagaact
    15961 tccggtcggg caccggctgg ccgttgaact tcgcgcttcg cagaatccag gttccatcga
    16021 gcgcacggag ggcatttccg gcgcgaacgg cgtccggccg ggcgggagcg acgtccccgg
    16081 gcgcggccgc ggcggcgctg cacgaggcgg cgagaagcgc gatcagaacg gctttcttca
    16141 tggtgtgcgt cctcctagcg ccgcgacgcg atctcggcga gcgcgcgaac tgagccgaag
    16201 aagcccgcgg agtcgaggga atcggcgggc agatccacgt ggaactccag gtggggctcg
    16261 tttcggcgca ctgcgaggcg gcgcaccgtc gcgccggccg tccgggcctc ccgccagagc
    16321 tcgtccgccc gcgcgagggc ctcgtcgaac ttcgtgaaga cggcgaggcc gccgggctcg
    16381 gacggttcgc gcacggacca gcgctcctcc ttcatgccgc ggagcgcgtc gcgccccgac
    16441 cagctcgcgc gatcgagcat ctcgacgtcc gcgagcgggt gcttccggag gcgggcccgg
    16501 aggatgagcg tgtcgcgccg cccgcgggcg cgggagatcg cccagagcca cggcaggtcc
    16561 cgcggcgcca ggaaaacgac gagagaggcc gactcgaagg gctcctttgc ctgcgcgagg
    16621 ccgaggtcca cggccgtcgt cccgatccag cgcacggtgg ccttgtcgcc cagcgccttg
    16681 aggccgccct gcagccactt cagcgcccgg ttgccccgct tcacgttcgc gccgctcccg
    16741 agcgcgaacc agccgaggac gaaggccacg agggccagaa gagcctgcga catctcgttc
    16801 gagcgacttc caaaagacga tagcagacgc gcccgcgtgc tagcgtcacg tcccggaggc
    16861 gacgtggagt ccacgggctg gaagaaggcg gcgcgggagt tcctctacgg gatgttcctg
    16921 cacgagcacg tcgaacaggc gcgcgacgcc cgcggcgcac tcgagagcct cttcctcgtc
    16981 gtcgtcttcg gcgacatggt cgggctgccc gtcctgccgc cgtattacgc gctccgtctc
    17041 ctgccccaca cgctcggcgc gctgccgggc tggaagcgcc ggatcctccg tgagcgcggc
    17101 ctcggggacg accacgagca ccatctccac ggggtctgac gccgtttccc gcacgatttg
    17161 atgcatctgc atcgggccgc ccgccgccgc agcccctagg ttctagggag cggacaagga
    17221 gggccggatg ggcgagaagc gggccgagcg ggccgggggc ttcaagggcc tctggcaggg
    17281 cttcaaggag acgatctacg ggatgtcggc tcacgacatg agccgcgcgg cgctccggac
    17341 gcgggcgtcc atggagcacc tcttcatcct catcacgatg ggcgaccttc tcgggatccc
    17401 gatcctcccg ccgtactaca gcctccggct ccttccccac gtggtccccc agatcgcgac
    17461 gtggaagcgc cggatgctcc gcgagaagga cgtctcggac gcgatgttct gagtccgggc
    17521 gaacgtcctc gacgcgatcg aacgggaggc accatgggca tgaaggaagt cttcgagcag
    17581 aatcccggcc gccgctacat catgttcggc ggcaagggcg gcctcggcaa gacgacgttc
    17641 tcggcggcga cggcctactg gctcgcgtcg cagggcaaga aggtcctcgt cttctcggtc
    17701 gacccgcagg cctctctgac ggacatcttc cagaaggaca tcttcgggaa gggcccggtc
    17761 aagatcatgg agaacctctg ggcgcaggag atcgacgccg acagccacat caaggcctac
    17821 caggacgaga tccgcaagaa gatccgcgac atgtacggcc tcgcggaagt gcccgaggag
    17881 atcgagagct acatccaggc cgcgagcgcc gagcccgcca tggaggagag cgcgatcttc
    17941 gacgccgtcg tggacgtcgt cgtccagaag gactacgact actacatcta cgacctcgtc
    18001 ccgctcggcc acgcgctcta ctacctctcg atggccaagg tctacgacga gtggatcaac
    18061 aaggtcacga agctgcgcga ggacatgcgc gagtacgagg agatggtcag ccgcctcaag
    18121 cgcgagaagg agaccgagca ggacgagatc ctcaaggagc tcgtctacat caaggggcgc
    18181 atcaacgcgt cctcgaagat cctcacggac aaggacatga cggcgttctt cttcgtcgtg
    18241 acgcccgagg acatgatcat ccgggacacg gagaaggccg cgaagctctt cgcccagttc
    18301 gacgtcccga tcggcggcta cgtcgtcaac cgcgtcatcc cgcacgagct gatcgaccag
    18361 aacatccccg actacctgcg caaccggatc cagatgcagg acaagtacat cgggaacatc
    18421 aagacgacgt tcggcaagga cgtcctcgcc tacgtcccgg agctcgagcg cgacgtcacg
    18481 ggcctcccga tgatcgagaa gctcgcgaag atcatgtacg gcgaccagaa gctcggctga
    18541 gaggaggcgc catgcccgaa atccagacct ccatgagcgc ctacatcaag gagcacgccg
    18601 gcctgaagta catcttcttc ggcggcaagg gcggcgtcgg caagacggtg ttcgccgcag
    18661 cgaccgcggt gcacaacgcg cagctgggcc ggcggacgat cctcgcgtcc acgaaccccg
    18721 tccacagcct ctcgagcctc ctcggccaga acgtcctcgg caagcacacg cccgtcgagg
    18781 gcgtcccgaa cctgtgggcc tacgagatcg agacgaagga gacgatcgaa cgcagcaagg
    18841 tcgagatccg cgagaagatc cagtggttcc tgaagttcgc ggacatctcc acgaaagcgg
    18901 aggacttcgt cgagtccgcg acgatgaacc ccgcgttcga ggaatcggcg atgttcgaga
    18961 acatgatcga cctcatgttc aagaacgagt acgacgtcta cgtcttcgac acggcgccca
    19021 ccgcgaacgc gcggcggctg ctcgggatgt cgaaggtgta cgggctctgg gtcaacaaga
    19081 tgctcaagag ccgcgaggaa gcgcggacgc tgcgcgagat gctgtcgttc acgaagaaga
    19141 aggagcagga cccgctcatg gcgtacctca tctccttccg cgaacggatg gagcacggcc
    19201 gggtcctcct cacggacccc gagaagacgt cgttcttctt cgtgacgctg cccgaggcgc
    19261 tcccgatcgc cgtcatcacg cgcttcatcc agtggttcca cgacttcggg attcccgtcg
    19321 gcggcgtcct cgtgaacatg ctgatcgaca agaagtccct cgacgcggga gcggcagact
    19381 tcgtgaagaa ccgcgtcgcg atgcaggacg agcacatgaa gaccgtctgg cagaagttcg
    19441 acggcagcgt ccgcgccatc gtcccgctct acgagacgga gatccgcggc gtcccgatgc
    19501 tcaaacgcct cggcgacgcg atgttcgcgt aggcaccgcg ggagcgcaga tgcgtctggg
    19561 tccggccctg gccggcgcca ccctcgcaat ctccctcgcc ggctgcgcga ccgtcggccg
    19621 gcccgacctg tcctctcaat cctgcgggga cgaactcgcc cggcagatct ccgacgtcct
    19681 cttcgcccag ggagaatcgc ggcccatctc ggacgacctc gcgaacggcc tcaccggcga
    19741 cctgagcacc ggcgcgctca gccccgtgag cttcttcgcg gactctccct cgggatgcca
    19801 gtacggcttc gcgattcacc agacatccga gggctgcgtc ctcaggctgt actaccggaa
    19861 gagaccgggt tacgaggtca cgaacaacat cacctacatc gagacgcgcc ccctcaaggg
    19921 gtgcacctgc agccgatagg aggcaacccc atgaacatgt ccctgcgtcg cgtgctccca
    19981 ctcttcgtgc ttctcgccct cgccgccttc cccgccgccc gcgctctcgc ggcgacggac
    20041 gaggagacca tcaaggtgtt cagggacgcc ggcgaaagcg gccagttctt cgacaagtgc
    20101 tacggctacg ccgtcttcgc caacgtgggc aagggcggca tcggcgtcgg ggccgcgcac
    20161 ggcaccggcc gcgtctacga aaagggcaag gtcgtgggcg acgtgaagat gaaccaggtc
    20221 tcggtcggcg cccagctcgg cgggcagtcc tacagcgaga tcatcttctt cgaggacaag
    20281 cgcgcgttcg acgagttcac gagcggcagc ttcgagttcg gcgcggaggt gtccgccgtc
    20341 gcgatcacgg ccggtgcggg cgcgaaggcg ggctcctcgg gctcaagcgc gggcgcgagc
    20401 ggcggcaagc acgacgccgc gacggccggc ggctaccaca agggaatcgc ggtcttcacg
    20461 gtggccaagg gagggctcat gtatcaggcc accgtcagcg gccagaagtt caagtacacg
    20521 ccgaggaaga agtagatacc gggttccgac ccgccggaac ttcaggcatc cttcgaggca
    20581 ggaggtcgac gatgccgagg ttcctgatcg aggttccgca cgaagaggcc cagttggcgt
    20641 gcgcccgagc cgtcgaggtc tttctcaagt ccggctccca ctacctgacg cacgccgact
    20701 ggggatgcaa ggacggggac cacaggtcgt ggattgtggt ggaggtcgac agcaaggacg
    20761 cggcccgggg cattctgccg ccggccttcc gggcccaggc acggatcgtc cagctcaacc
    20821 tgttctcgat gcccgagatc gacgaaatca tccgccggca caaaccctga cggaccggtc
    20881 tcaccggact cctgcgacgc gatatcaacc ggatcgcggc cgccccgcgg agtaggcttc
    20941 gagtccggaa cgccgatgcc gtccctccat ccgaccgagc tgaccatgct gctcgacctg
    21001 agcgaggcgc tcgggtcgcc cctgaacctg cgggcttcgt tcgcgcgggc cctcgagatc
    21061 ctcgagacgg acctgaaagc ggtcttcgga atggtcgcgt tcttcgaggc cgaaaccgga
    21121 gagctgaagg tggaggccgt cacgggccgg cacgacgcgg ccgcgcgcaa ggcgcgctac
    21181 cggctgggcg aggggatctc gacccgcgtc gtcaagacgg gaaggccgat cgcggtgccc
    21241 cacgcgagcc aggagccgct cttccgcgac cagctcggcc tcctcgcgtc gcgcaagtcg
    21301 ccgaaggacg atctctcgta cacctcggtg ccgatccgga tcgacggaaa gagcgcaggc
    21361 gccctcgccg tggcgttcgt ctacgagaag aaccgggacc tcgggcacct cgtgaaggtc
    21421 ctcgggatcg cggcctcgat gctgggccag gccgcgcgcg tgcaccgcct cctcgagggc
    21481 gagcgcggcc gtttgatgga ggagaaccgg cagctcaagg agaagctccg cgagcggtac
    21541 gacgtcggga acctcgtcgg gacgagccac ccgatgcaga agctgttcga ggagatcgcg
    21601 caggcggcgc ccgcgaacac gacggtcctg atccgcgggg aatcgggaac gggcaaggag
    21661 ctcgtcgccc acgcgatcca ctacaactcg cctcgggcgg agaagccgtt catcaaggtc
    21721 agctgcgccg cgctgccgga gagcctcatc gagtccgagc tcttcgggta cgagccgggg
    21781 gccttcacgg gcgccgagaa gcagaagaag ggccggttcg agctggcgca cggcgggacg
    21841 ctcttcctcg acgaggtggg cgacctcccc gccgccacgc aggtgaagct cctccgtgtc
    21901 ctgcaggagc gcgagttcga gaggctcggc ggcgtcaagc cggtccgcgt cgacgtccgg
    21961 ctgatcgcgg ctacgaacat cgacctcgag gaggccatga aggaaggccg gttccgcgag
    22021 gacctctact accgcctgaa cgtcttcggc ctcttcgtgc ccccgctccg cgagcgccgg
    22081 tcggacatcc tcctcctcgc ggaccacttc gtggagaagt acgcggccgc ccacgggaag
    22141 agcgtgcgaa ggatctcgac ctcggcgatc gacatgctca tgagctacca ctggcccggc
    22201 aacgtgaggg agctggagaa ctgcatcgaa cgcgccgtcc tcgtctgcga gggcggcgtc
    22261 atccacgcgc accacctccc ccccaccctc cagacggccg aggtctccaa cacgcttccg
    22321 ggccaggcgc tcgcggatgc ggtctccggg ttcgagaagg atctgatcct cgacgggctg
    22381 aagatcgccc gcgggaaccg cgccaaggcc gcccgcctcc tcaggacgac cgagaggatc
    22441 ctgaactaca agatcgggaa gctcggaatc gacgcttccc gcttccggcc ctagcaggac
    22501 tcggagaaga agaagagaat tcctacgaat atgtagggcg gatgagacca gccgacagtg
    22561 ttgtacccga gggcccttgc gtgtcggcgt tcgaggccgg gaggcgcgtc gaaggccagg
    22621 gcgcttcggc ctctctcgtg ggtgcccccc ttcagttcaa tacacattcg tagctcttct
    22681 cctcccatct gacaatcctg tcttcgattc gcctggcacc ggacgtggcg ccgacgggcc
    22741 ccaagccact gcccctcaat cactttcaaa gaatcccctc ttctcgtctc ccccggcacg
    22801 cggcctgcga aggcgcccgg caacgggagg cgacgatgaa gaacaagaaa ctgatcacgg
    22861 tccttttcct ggtcgggctg gtggcgacct cgtgggcgct cctggccgcc gacccgtcgg
    22921 gcgcgaacac gggcgggatc gcgaacgtgc cgtcgaaggt tgccggcaag ccgacggtcg
    22981 aggagatcgg cgccgccgtt ggccagacgc gcgtttccct caacttcgtc tggacgctga
    23041 tggccggctt cctcgtgatg ttcatgcagg ccggcttcgc gctcgcggag acgggcttca
    23101 cgcgcgcgaa gaacgccgcc cacacgatga cgatgaactt catggtctac ggcatcggga
    23161 ttctcggctt ctggatcgcg ggcttcgccc tccagatggg cggctcgggc gccgcgatgg
    23221 gcgcggccct ctcggttccc gcggaaatgg gcaagctcat cggcccgacg atcggcggaa
    23281 acgtgtgggg cctattcggc ggcaccggct tcttcctctc cggcgtctcc tacgacgtcg
    23341 ccgccttcgc gatctttctc ttccagatgg tcttcatgga cacggccctc acgatcccga
    23401 ccggctcgat ggccgagcgc tggaagctgt cggccttcgt catctacgga ttcgtggcgt
    23461 cggcgctcat ctatccgatc ttcgccaact gggcctgggg cggaggctgg ctctcgacgc
    23521 tcggcaagta cgccggtctc ggccacggca ccgtcgactt cgcgggctcc ggcgtcgtcc
    23581 acctcacggg cggcgtgatg gcgttcgtcg gcgcgaaggt cctcggtccc cgcatcggca
    23641 agtacgtcaa gggcgaggtc cgtgcgattc ccggccacaa cctcccgatg gcgttcctgg
    23701 gctgcttcat cctcgcgttc ggatggttcg ggttcaacgc gggctcgacg ctcgcgggca
    23761 cggacctccg gatctcggtc gtcgccacga acacgatgct cgcctcggcc tcgggagccc
    23821 tgctcgcgtt cctcgtgacc tggaaaaagt acgggcggcc cgacccgtcg atgagcgcga
    23881 acgggatgct cgcgggcctc gtcgcgatca ccgccccctg cgcgttcgtg aacgcgcccg
    23941 tggccgtcct gatcggagcg atctcgggcg tcctcgtcgt ctacgccgcg ctctacatcg
    24001 agaacagact caagatcgac gacccggtcg gcgcgatcgc cgtccacggc gtcaacggcg
    24061 cctgggggct gatcgccctc ggcctcttcg cggacgggac ctacggcggc ggcctgaacg
    24121 gcgggcccga agccggcgtg gccggccttc tctatggcga cggccggcag ctcgtcgccc
    24181 agctcatcgc gatcgccgcg aacttcgtct gggtcggact cacgagcttc gtcctcttca
    24241 aggtcctcga cgccaccgtc gggatgcgcg tctctcccga gcaggagatc cagggcctcg
    24301 acttcaacga agtctccgcg cccgcatacg cgggtgaccg tccgacggtg aacacgccgg
    24361 tcgtcgtgtc ccggccggcg gccgcggccg tcgccgtcct gaagccggcc cccgccggcg
    24421 ggaagtagag ggaacgccat gaaactgatc accgccatca tccgtcccga gaagctcgag
    24481 gacgtgaagg tcgcgctctt cgcggcagag gtcgaggggc tcacgatcca gcaggtctcc
    24541 ggccacggcg gggaaaaagc cgtcatggag agctatcgcg gccacccgct gctctacgag
    24601 ttccacgaca aggtgcggct cgacatcgcc gtctccgaac cgttcgtgca ggtcaccgtc
    24661 aaggccatcg ccgacgcggc ccggaccggg caggtgggcg acgggaagat cttcgtccag
    24721 cagctcgacc acgtcgtgag gatccggacg ggcgaagccg acgtcgaggc cctgaccccc
    24781 gtgacggtcc tggcgaaagg ctagcgagga ccccatgaaa acgccctttt cgggcggcct
    24841 tcgggccgcc ctcgcgggag ctctgtttct cacgaccgga ctcgcgtccg ccgccgacgt
    24901 cgtccctccg gcggtgcttc ccccgaatcc gcctgttccc gcgccgacgc cggctccggc
    24961 gcccaagccc tggttcgagg agatctccgt caacgccttc gtctcggcga gctactcgta
    25021 caacttcaac cggcccgaca ccggtacgaa ccagctccgc gtcttcgact tcgacgacaa
    25081 ctcgttcaag gtggacgtcg ccgaaatcgt cgttcagaag gccgtgagca agcccggaga
    25141 agccgggttc cgcgtggact tcgtggcggg ctcctcgatc ccccgcgtct cgtcgtccta
    25201 cggcctgctc cagggccagg acgtcgacct ccagcaggcg ttcgtgagct ggatcgcgcc
    25261 cgtcgggagc gggctgcgcc tcgacgccgg aaagttcgtc acgcacctcg gctacgaggt
    25321 gatcgacggg tacgacggct ggaacgacaa cgcgacccgc gggttcctct tcggattgac
    25381 actcccctac gcgcacacgg gcctgaaggc gacgtacgcg ttcagcgacc agctggccgg
    25441 gatgctcgag atcgtgaacg gctgggacgt cgcacgcgac aacaacacgg ggaagagcat
    25501 cggtgcgcaa gtcgcgtgga cgccctcgaa ggccgtcacg gtcgccggga acttcatgac
    25561 ggggcccgag cgcgccaacg tgaactcgga tccacggacg acgttcgacg tcgtggcgca
    25621 gtggaagctg agcgacctga ccgccttcac ggtcgaggcc gtgtacggca gcgagaaagg
    25681 cgccgtgaag cccggcgaga cggcctcctg gtccggggtc gccggctacg cccgcctcgg
    25741 gctcagcgac accttcgcct tctgcctgag ggccgagtac atcagcgacg cggacggcgc
    25801 ccggacgggc acggcgcaga agctcacgga ggtcaccgtg acccccgagt ggaaggcggc
    25861 gcctcacctg gtcttccggg cggacctgcg cgtcgactgg tcgaactccg acgtcttcgt
    25921 caagaacgac ggcaccttca agggaaacca ggcgacggta ctcgtcaacg ggatctacgt
    25981 cttctgatcc gctctacgac ggcggctctc ggcgtcgtga cgtggcgccg agggccgcga
    26041 ccgcggtgtc gtggatcggc gtctcgacgc cgacctcgcg gccgaggcgc gagacggcgc
    26101 cgctcaggtc gtccagctcg gtcggcccgc cggcctcgag atcgaggagg aagctcggct
    26161 tcatcccggc gccgaggccg tcgacgaacg cgaggatctt cgcctcctcg tcggccgcca
    26221 gggcgacgcc gcgcgcccgc gccaccgcga ggacctcgcg gacggcgcgc tcgaggagca
    26281 gcttcccgta gggagcctcg cgcacggggc cgatcgcggt tcgcgccagg ccgcaggcgg
    26341 ccgccatcgg cgcgatgaac gcgaacttcc gccagaggtc ggcctcgatc tccagcgaga
    26401 cgcgggcctg agcccccgcg tcccggaacg cttccgcgat ccgctcgccg cgcgcggacg
    26461 gcccgccttc gagctcaccc accgcaacgt tctggaacgg actcttccgt tcgacgacgc
    26521 ccggcgccac cttcgcggcg ctgatctgcg tgagtccccc gaggacgcat tccttcggca
    26581 cgccgcctgc gacgaggcga gccgcggcct cgactccgtt gaggagcggg aggaccgccg
    26641 ctcccgtctt cgcgagcagg cgcgccgcgg ggagcacctc ggcgagcgaa tacgtcttca
    26701 ccgccacgat cgcgaggtcg gccggaccga gcgacgcgac gtcgttcgag gcctcgaccg
    26761 ccacggtgaa gtttccgtcg ggcgtccgga cttcgagccc ccgcgcgcgg atggcctcga
    26821 gatgcgtgcc gcgcgcgaga agcgcgaccg gatggccggc ccgcgcgagc gcgcccccga
    26881 agtagccgcc cacacccccg gcgcccagaa cggcgacccg cagcttcccg cccatgcggg
    26941 cgagtctagc gccgcgatca gcgcccttcg atccttcgtt tgaactcctc gaggacagag
    27001 tgcgggtcgc gcgctgcggg catttccatg cacacgtcgc acgtctgcac cttgatcgca
    27061 cggacgtccg cctgcccgaa ggcctcgacg agctgcgaaa gccagaacgg gacgcgctcg
    27121 agcacggcga agtcgatgcg cttcacggag tggcaccgcc cgcacagaag agtcaggaca
    27181 tcccccggca cgcccggacg ctattgaatc ggaccgtcag gcggcatgaa gcgcctgcat
    27241 cagcgacgcc ccggggtcgc gttcagcgcg acccgaagcc gatgacgtcg atgcgagtga
    27301 ggattccgac cggccggcct ttttcctcga cgagaagcgc gtgcgccgac gtcaggatcc
    27361 gcgtcgccgc cgccaggggc tcgtcggagc cgaggcgcgg cagcggcgct tccatcgcgg
    27421 tcgagacggc ggcgtcgggt gtcgctcggc cctcgaggac ggcctcgaag agatgcggct
    27481 cgacgacggt gccgacgact tctcccgctg cggacatgac cggcagctgc gagacgtggt
    27541 gcttgcggat gagcgcgagg gccgtgcgca cggggtcctc gggcttcagc gtcaggagcg
    27601 gcggcaggtc gccggcggcc ttctggcgca ggaggtctcc cacgcgcatc tccgcggcgt
    27661 cgagcatccc gtggtccgcg agccagccgt cgctgtggaa cttgctcagg tagcgttcgc
    27721 ccgtgtcggg cagcatgacg acgatcacgt cgtccgccgt catccgctcg gcgatcttca
    27781 gcgcccccgc gagcgccgtg cccgaagagc cgccgacgaa gatcccctcc tcgcgcgaga
    27841 gacgccgcat gaggtggaac gactcgcggt ccgtgacggt caggatctcg tcgatgtact
    27901 tgaactcgac gttgccgggg atgatctcct ggccgatccc ctccacgagg tacggggcgg
    27961 agtcgaccat cttcccggtc cgctggaact cgccgaggat cgacccgacg ggctcgacgc
    28021 cgacgacgcg gacggccggg ttctgctcct tgaggtagcg ccccgcgccg ctcgtcgagc
    28081 cgcccgtgcc gagcgaggcg acgtagcagg tcacgcgtcc gtccgtgtcg cgccagatct
    28141 cggggcccgt cgtgaggtag tggatccccc ggttgtgcgg gttgaagtac tggttcgcga
    28201 ggaacccgcc gggggtctcg gccgcgatcc tcttcgcgac ctcggtgtag cgctccggcg
    28261 atccgggagg cgcgctcgtc ggcgtgatga cgacgcgcgc gccgtgcgcc ttgaggaggt
    28321 cgcgcttctc ctgcccgacc ttgtccggca tcacgcagat gcacttgtag cccttcacga
    28381 cggccgcgag cgcgagcccg aggcccgtgt tcccggacgt cgcctcgacg atcgtcccgc
    28441 ccggcttcag cgtcccgtcc ttctccgcca tctccacgat gtggatcccg acgcggtcct
    28501 tgttgctctt gcccggattg aaggactcga gcttcgcgag gacgagcggc ttcagcccct
    28561 tcgtgacgga gttcaggcgg acgagcggcg tgtcgcccac cgtcgcgaga atgtcttcga
    28621 aaaagcgcat cgcgttcaga gcgggaggcc cgggtcgacg tcgcgcgccc aggcgaggat
    28681 cccgccggcg agattgtacg tgcgactcag tccggccccg cgcaggagcg cgacggcccg
    28741 cgccgagcgg accccggagc ggcagtacac gacgacgggg cgcgaccggt ccacctcgtg
    28801 gaggcgggcc ggcagcgagc cgagcggcac gaggacggaa cccgcgattg cggcgagcgc
    28861 ggcctccgcg ggctcgcgca cgtcgaggag ctggaagggt tcccctctct cctgccacgc
    28921 ccgcaggtcg gccggcgaca tctccgcgcc ggcgggcagg acgggggcgc ccgcgccgca
    28981 gagggcgtcg tagtcgacga gatccgtgat cgacggccgg tcgccgcaga gcgggcacgc
    29041 gggatcgcgc ggcacggcga tctcccggaa cgagagcgca agcgcgtcgt acaggagcag
    29101 ccggcccgcg agcgaccggc cgatcccgag cagaagcttc agcgcctccg tcgcctgcag
    29161 ggcgccgacg acgcccggca ggacgcccag caccccgccc tcggcgcagc tcgggacgag
    29221 gcccggctcg ggcggcgacg ggtagaggca gcggtagcac ggccccttcc cgggctgaaa
    29281 gacgctgacc tgcccctcga accggtagat cgaaccgtag acgttcggct tgccgaggag
    29341 gaaacacgcg tcgttcgtca ggtagcgcgt cgagaaattg tcggtgccgt cgaggacgac
    29401 gtcgaacccg tcgaagacgg tccgcacgtt gtcccgcgcg aggcgctccg ggaagacggc
    29461 gacgtccacg tgcggattca gggcggacag gcgctcgcgc gcgacgtcga ccttcgggcg
    29521 gccgacgtcc cgggtgtcgt agaggacctg cctctgaagg ttcgagacgt ccacggcgtc
    29581 gaagtcggcg aggccgatcc gtccgacgcc cgccgcggcg aggtagagcg cggcgggcga
    29641 gccgaggccc ccggcgccga cgacgagaac gctccccgat cggagccgcg cctgcccggc
    29701 cgcggacacc tccggcagca ggaggtggcg cgagtagcgg gcgatctcgt cccgggagaa
    29761 gggctcggac atcgcgtgca accttacacg ctccacggcc tcccccggga cccggcggct
    29821 cagttgaagg gcgtcccggc cggacccatg attcggattg ccgggtaata ggacaccgtc
    29881 cgcttccagt ccacgacgag ttccggaagg gctggcctgc cgacctggta gacgtccgtg
    29941 tgcggggttg cgtcgttttc gcccccgcac ggtccggacg tcggcccgca gagcttggtg
    30001 aagatcaccg agtggaacga ccacccgttc cggagagcga cgttgaaccg gatccggtcc
    30061 gtccccgcca cgtccgtgca cgaggcactg acgagagcac cggtgtgatg aacgatgccg
    30121 tacccgaagt tcttgtggca tccctcgagg acccagcctg tcggcgagag ctcggccaga
    30181 tccaggagag gcttgagcgt gatcgccttc gacggcgcgg tcttcgtcgc ctcgacggtg
    30241 accaccttga gtgtcacggt cttcgggtcg gcgaaccccg tgaggtgcgg aagggacgct
    30301 tcgatcgcat tgtccttcca gctcgtgaca gtgagttgct ggttgccgtc cgagaggatc
    30361 accattccct tgctctctcc gaacccgcag cccgtgatgg ccacggggtc gtccggcatg
    30421 gcgaggtccg gagtcacctc gtagatcttc ggcgtcgtct tgcaggccgc cgcgtccgcc
    30481 gccgccttgc tgattgcgac ggcgcgccgg gcctggaact ggcttcggag cgcctgtgtt
    30541 ctcgccgttt gcttgcccgc ttcctgacct ttcaggctct tggtctcctg atccgcaagg
    30601 gccttcagtc ggaccccgat ctgttccgga gagaccgccg gcagaacggg cgccgcaggt
    30661 ttctgggccg cacccgggag ctccgccggc gccgtcccgc tccacgtggc caggcccagg
    30721 accgccgcta ccatcgagac cttcgcgttc atgacgtccc ccttcgcgtt gctccttcct
    30781 gcaagttagg ggcgtcgtca ctggcccgca aggagcatcg gtgccgacga cggggagccg
    30841 tgcgacgata ggcgccatgg cacgcgtcaa gggagcgcga tgatcgcgcc cttctcgagc
    30901 aagacggtcc tcgtggccgg ggggacgggc ggcctcggcc gcgccgtcac gctcgccctc
    30961 ctcacggagg gcgcgcgcgt gtccgtcacg tatcgcaagc gggaggagct cgtcgtcctc
    31021 aaggccgccg ccggcgccgc tcacgggcgc ctcgagggat acgaggcgga cgtgaccgac
    31081 gagacggcgg ccaggggcct cgtcgaggcg gtgcgggcgc gccagggccg cctcgacgtg
    31141 ctcgtgaaca cggtcggggg attcgcgggc ggaaagaagc tctgggagga ggacgcctcc
    31201 ctcctcgacc ggatgctggg cctcaacctc cgctccggct ggacgctcgc ccgcgccgcg
    31261 gtgcccgtca tgctcgcgca ggggcgcggg gccatcctga acgtcgcggc ccaggccgcg
    31321 ctcgccccgc ccgcgggcct cgccgcctac gccgcctcga aggcggccgc cgtggccctg
    31381 ttcgcctcgc tcgcggccga actcgccggc tcgggcgtgc gcgtgaactc gatcctgccg
    31441 tcgatcatcg acacgccggc gaaccgaaac gccatgccgg ccgcggactt ctcgaagtgg
    31501 ccgaaacccg aggaaatcgc ccgcgtcgtc ctcttcctct gcagcgacga ggcgaagctc
    31561 gtgaacgggg ccgcgatccc ggtgtgacgg gagttccgcg tcagcgcgga acgattgccg
    31621 acggctcgac cgccgccggg aagagcgccg ggtcgtggcg gatgcgcatc agggcgtcgg
    31681 ccgtctccgg cgacgtcttc cggagcagcg cggcgaggtt cgcgcgcgcg ccttcgtcga
    31741 ccggcacctt cgacacggcc tcgacggtga cggccgtccg aggaaaccgc ttctcgccgc
    31801 cttccctgag gacgacgtcc gtcatgaggg agttcggatc cagcaccttc acgcccggat
    31861 ggccggccgc cgcgaacgcc tgctcccaga gcggcttcac gtagccgaag tgcgtgcagt
    31921 tgaggctcac gacgagcggc ccgcccttcg tcgtcttctt cgccagcgcg tccgcgacga
    31981 acttgttcac gtagcccgcc gtctcggcgc tggccgagcc ccgctcgatc gcgcccgcga
    32041 gcttcgggca ggcctcaccc acgacgtggt ccggcgcgac gccctgggcg acgagaagcc
    32101 tcttgtgggc ctccgactcg atcgtcgtct tcgtcgcaaa gacgagcgcg gtcgcgtcgg
    32161 gcgtcgccgc gagctcccgg cggatgagtc cggcgccgag gcccacgatc tcgaccgtct
    32221 cgacgccgcc cgtcttcgcg tgctccgtcc gcggccagac gaccgagagc gtgttgcacg
    32281 cgatgaggat gagatccggc ttccagcgcg cctccatcgc cgagagggcg ccgtcgaaga
    32341 tgcggacctt gtcggcgtcg ttccggaggt cgttgtagcc gaatgcgggg tcgggctcgg
    32401 cgttcacgaa gacgatgcgg gcggacttca cgatcccgct cgcgggcagc cgggccgcga
    32461 ggtcggccgc gacggagagg ccgccgaggc cggagtccgt gacgacgatc gtgacgtcgg
    32521 gcttccggcg ggccgcgtcg gcgaagcccg ggggcttccc ggcgacggcc caggccgccg
    32581 cgagaacggc ggcggcgagc gccggcagcc gcacgcgacg cgtcacctcg cgagctcctc
    32641 gagacgcgcc gggacgtcgg aacgccggag ttcgtccagc ggccagagcg ccatggaccg
    32701 gtctgcgtag agcccgtcct cgaggcggct gccctcgtcg atccgctccg ccggcggcgg
    32761 aaggtggaac ggggcgcgcg tgaggttgat cgggcggaag tctccggacg ggatgtcgag
    32821 gttttccttc cggtcgaagt agtggctcgc gagaagcaga cgggatccgc tcctcaggac
    32881 gttcgcgagc gcccgggccg cgtcgcgcgt gccgaggtga atgaggaggt cccggcacag
    32941 gatcaggtcg acgttcggaa gcgggtccgc cgtcacgtcc agcacgcgga actccagcgg
    33001 cggcgggaac cgccgccggt tggactcgat caggtccggg acgatgtcgg cgccgacgta
    33061 cgagacgccg gacaggtcga gccggctcat ccagtggaag tccccgcacg gcacgtcgag
    33121 aagcgacgtg acgcggaatc gccggaacag cgccgggagt tgccggcgca gggactccgt
    33181 ctcggccagc gaggagccgg cgccggaaag gctctcctcg gcgtggtaga tgccgagttc
    33241 gtggaggagc cggaaccgtt cggccagggg gagcgagtcg acgacctcgc gcagccgctg
    33301 gatggtctcc gcgcgcgaaa gccccttgaa gtcggcggcc gtcgggccgg agacaccttg
    33361 ctccactcgc atccccctcg ccgcgagctt acttcagacc gtcccgacag ggcctactcg
    33421 agcgggatgc gcttcgcgtt gcgcgcgagc cactgctcga agttctggag ccccgggtgg
    33481 agcttccggg agaactcgac gctgcgcgcg gcgcagaagt cgtcgctgaa gtcccggttg
    33541 aactggaaca tgttgccgag gtcgtcggcg cccgggaatc cgaagccccg gtagacgtcg
    33601 ggggtgacgg cggcgtagcg cacctcgcgg cccagcgcct tcgagagacc ggccgccatg
    33661 ccggcgccgg tcacgtgctc gccggcgatc ccgacgctct tgccgatgta cgtcgcaccg
    33721 cccttgaaga tcccgtaggc gcacgcgccg atgtcctcgg cggcgatgcc gggcagcttc
    33781 ttgtctccca tcggcagcgc gatcgcgagc gtgccgtcgg gtcccttctt cgggcccatg
    33841 ccgaaaaaga cgaagttatc ccagtagaac gacgtgtaca ggaacgtcgt cgggacgccg
    33901 agatccgtga agaagtggtt ggattcgccc ttcgcgtcga agtgcgggac cttgtacttg
    33961 ccctgcagcg tcggcatgcg ggtgtccgag agcggaatcc acttgcgtga atcctcgagc
    34021 gtggaccaga tcgcgtgctt cacgcccgcg tccttcgcgg ccttcgccat ggcggccgcg
    34081 tgcgccatct ccttctcggg ggagaagtgc gcccagaaga acgtgacgca ataggcgccg
    34141 tacgcgcccg tgaacgcctt ccggaggctc tcgggatcgt ccacgtcgcc cgcgacgacc
    34201 tccgcgccga gcttcgcgag ctccttcgcc ttctccgagt tcacgtcgcg cgtgatggcc
    34261 cgggcggcga agccgccgtt cgggtccgcg aggatcgcct tgacgagtcc gctgccctgc
    34321 gcgccggtgg cgccgacgac ggcgatgatc ttcttgtctg ccatgggttg tctccttttc
    34381 gggtttatcg ctgttgtgga atcgcggcgg cttcccgcgc cgtgcggatc gcgagccgtc
    34441 cgcgcacgtc gtcgagcagc ttgatgaggg tcttcaggcg cgagggcggt aagggcgcga
    34501 gcttggccaa ggtggcctcg atcgcctcgt ccagctccgc aagggtcttt cggccccgcg
    34561 ccgtcgcgac gacgcggacg acacgccggt cgtccggcat cggccggcgt tcggccagcc
    34621 cgctctcgct catcttgtcg atgaggcgcg taatgttggg gctgcgggcc agcatccgct
    34681 cggcgatctc cagggtcggc agcccgtttg cgtccgcgcc gcgcaggatc ctgaggatgt
    34741 tgtattgctc cggcgagact ccccagggcc tgagcgcgca ctccaccgcg tgacggagga
    34801 cgtcccccgt ccggagcagc gtcgccgccg cctccctctc gaggctcgag aagggccgtg
    34861 cctgcgcgat ctcggcggag atcgtgccca cggcgggacg agaggacgcg cccgggagcc
    34921 ctgcagtctt gcgcgacctt ctcatcaccc gggaaattat tacctaggaa tgtaattgtc
    34981 aagcccgaac gccctccggg caggcgattc gttgcgatag aatctcagta gtaaccgtaa
    35041 ggagcctctc ccatgacgaa gcggcccgtg ttccggtgca agctccgcaa cgcattcgcc
    35101 ttgtccgtcc tgatcgtgct cgccgccggt tcggccggcg tttcgcgcgc ccaggctccg
    35161 ccgcccgcgc cggcgctcaa gccggtccga cccggaaagg ctcccgcgaa cccgtacacg
    35221 aggaagcaga tcgctcgcgg aaagctcctc gtcgtctcct cgggctgcaa cgactgccac
    35281 accccgtgga tcgtcgaccc ggagctggga gttccggctc cggactggac gcggatgctc
    35341 tccggccacc ccgagggcgg gccggaccct cagggcgaga tcgggccggc cgacatcggc
    35401 ctcatcggcc cgacgttcac gagcttcaag ctgccgttcg gcatcacgta ctcgatgaac
    35461 ctgacgcccg acatcgacac gggcacggga tcctggaccg agaagatgtt cctcgatgtc
    35521 ttccgcaagg gacggcacct cggaggcgac ggcaggcccg tctatccgcc catgccctgg
    35581 gattcctacc ggagcctctc agacgaggac ctgagggcga tcttcgcgta cctcaggtcc
    35641 attccgccgc tgcgaaacct gccgcccacc gagaaggtcc cgcccccggt ggaaggagcg
    35701 atcgtcgagc tcaacaacaa ggtcatcgcc ctccaggcga acccgaacgc gaagctcgag
    35761 gcgccgaaga acggcccgcc ccctcctccc ccgctcgccc tcaagccggt cgtcaagggc
    35821 aaggcgccga agcggacgta ttccgccgac atggtccgga aggggaagct gatcgtgacg
    35881 gcgggcgcgt gcaaccactg ccacacgccc tgggtctaca acccggtcct gggcgctccc
    35941 gcgcccgact ggacgcggat gctctcggga catcccgagg gcgcgcccga cccgcagggc
    36001 aaaccgggcc cgcaggacat cgcgctcatc gggccgacgt tcacgagctt ccagctcgcg
    36061 ttcggcaccg tctactcgaa gaacctcacg ccggacatcg agacggggag cggggcctgg
    36121 accgaggaga agttcctgaa gatcttccgc acggcccgcc acccggacgg cggcgtcctc
    36181 ctgcccccga tgccctggaa catgatccgc atccttccgg attcggacct tcgcgcggtc
    36241 ttcgcgtacc ttcagtcgat tccgccgctc cggaacgaag tccccatgtc gaaggtgccg
    36301 ccggaggcgc agaagatgat cgagggcgtg aacgcgaaga tgctcctgca ccccgcgggg
    36361 ccctactagt cagcgcttct tcgtcacctt cgcgacggtg atcttgaagc cgtcggggtc
    36421 ggtgagggag aagtcgcgca tgccccaggg ctggtccgtc ggcggctggt cgagcgtgcc
    36481 gccgcgcgcg acgatccctt tcgccatcgc gtcgatgtcc ccggtcgcca tccagtagag
    36541 gcggaagccc tcgcccttct tccggtcgcg gcccttcttc cagtcgtcct ggccgagcag
    36601 gacgaccacg ttgcccgcgc gaagctgggc gcccgccacc ttcccctcgt gcgtccacgt
    36661 ctcctcgacg gcgaacccca cgacgtcgcg gtaccagccg aggctcttct cgaggtcgtt
    36721 cgccgtgatg cccggcgcga cgctctccag cttccatccc ggcttcgcgg ccggcgcgcg
    36781 ccgtttcgcg ggagctttct tcgcagactt cgccttcttc ggggaggact tcgcggcggg
    36841 cttcttcgac ttcttcttcg aggccatgtc ccttctcctt ttcgggtgca tcctacgcgc
    36901 ggtagatcgc gaacaccgcg ccctgcggat cggcgaggac ggcgaatcgg cccgtgccct
    36961 cgatgctcgc cggaggaacg tacgttctcg cgccgagcga cacggcccgc gcggccgccg
    37021 cgtcgcagtc ggcgacggcg aagtacggca gccagtgtgc cggcacgcgc gcgccgtccg
    37081 ggggcgccat catcccgccg atcggcttgc cgccgagcac ccactccgtg tacgtcatgc
    37141 caccgcccgt gcccgtcttc gccctccagc cgaagagcgc cgtgtagaac gcctcggcct
    37201 tcgccgtctc gttcgtgttg agctgccccc agcagaaggc gcccggctcg tcctggacgc
    37261 cgatgcccct gttcgccttc gcctgccaga cgcagaagtt cccgcccgtc gggtccgcga
    37321 ggaccgccat gcgcccgaag tccatgacgt cgaacgccgg cgcgttgacc tttccgccga
    37381 gctcgcccgc gcggcgggcg gacgcgtcgg cgtccgcggt cgccacgtag agcatccagt
    37441 gcgggggcac gccgggcatc ttcgcggcct ccagggtgta gcaggcggct gcgtcgcgcc
    37501 cgttcagatg gaagatcgta taggtcatgc cggggcccat gtcgttgtcc gtaaaggacc
    37561 agccgaagag cttcgtgtag aagtccttcg ccgccttctg gtcggtcgtt ccaagctcga
    37621 tccagcagaa cgctcccggg gcgtgtgacg tgacctcggc catgtcccct cctgacgctg
    37681 agtctcgttc gagaatacgg ctccgagggg agtttgtttc cggcggggcg ccctcacttc
    37741 ccccgcgcgg cgtcgagact ccacggcccc gcgccggccg cggagaaata gagccagacg
    37801 aagcagaaga gaatcgccag ctggccgccg ttcacggtgg gccagaagcc cttgggcgcg
    37861 tgaaactgga aatacgccac cgccatctcg cctgcgagga ggaacgccac gggccgcgtg
    37921 aagaggccga gaaccagcag ggagcccccg acgacttcga ggacggcacc gagcccgagc
    37981 tgggagagga gcggcaccgt ccctccggcg ggcggaaccc cgatgggaaa ggcgaacagc
    38041 ttcatcgtgc ccggaaggat gaagagcgcg gcggccacga tgcgcagaat gctctgcagc
    38101 tgcggagccc gggaggacca tttcgaaaag ggtcccggcg ccttcatgcg accgccgtcg
    38161 cgaggtaggc cacgagttgc ccgaacgtcc cggcaaagcc cttctccatc gacccgatgc
    38221 cgccctcgaa ggccctgcgc tcggactcgg aggcgtggag cggccagccc tcgagcgtca
    38281 gcgtcgtcct cccccccgac gcctcgagcg tgaggacgtt caggatctcg agcggccagt
    38341 cggccgccat ggggtggcgc gtgacgccgc cgttctcgtc ggagaaggac acgacgaagg
    38401 cgaggcgcga gggctcgacg acctcccggt agacgaaccg gccccacatc gtcgtcttcc
    38461 cgtccggcgc cgtcatgccg tagtggaaga tgccgccggg ccgaaggtcc atcgtcccgc
    38521 gcgcccaggc gaagcccttc ggaccccacc acgcggagag ccgcgcgggc tccgtccacg
    38581 ccttccacac gaggtcgcgc ggcgcgtcga gcgtgcgcgt gaggacgagc ttcgggccgt
    38641 ccccgagcga ggcgaggtgc gcctcgagcc ggtccaggtt ctggcgctgg ccctggagcg
    38701 ccccgaagac gttcaccatc cggtcgcgcc gctcccgcgt cgcgaagacc atccgcgtcg
    38761 agacgcgcgt ctttccggcg ccctcgtccg tgaaggtgac cgtcacgtcg aaccggtcct
    38821 cgtcgcgctc ggccgagccg tgctcgtaga cgaggcgctc gggccggacg acctcgacgt
    38881 accgcgtgag gttcgggtaa tccgttccgt ccgggccgtg cagcgtgtgg cgccagactc
    38941 ctccggcccg cacgtccatc tcgtccgtcg tcacggcgaa gccgttcggg ccgtaccagc
    39001 gcgcgaggtg ctgcgggtcc gtccaggccc gccagacgag ctcgcgcggg gcgtcgaaca
    39061 cgcgcgacgt cgcgatttcc cggtcggccg tcgggctcga cgacgcgccc gcctcgttgg
    39121 actcgacgag cgccgtcgcg aggcgctcga gggactgcga ccagcccggc ttctggccgc
    39181 gcgccatcat cgggttgctc gggacggtct gccgaaccgt gaggcgcgtc ttcccgccct
    39241 cctcctcgaa cgtcacgacc gtctcgattc ggatctcgtc gtggtcgagc gccgaaaaca
    39301 cgaggcgcgc cggcgcctcc gcctcgcgga cgaagccctc cagcgggtac tcggttccgt
    39361 ccggcgcgcg catcacgagc gtgaacggcc caccggggcg cagctcgacg cttccacgcg
    39421 gcgtcgtgaa ccccatcggc ccccaccagc gcgcgagctg ggcgtgcgac gtccacgcct
    39481 cccagaccag cgctcgcggc gcgtcgaaga ggcgggagat gacgacctct cgcgcctcgg
    39541 gcacggcctc acgcacggcg gctcttgcgg gcatggcggg actccttttt tgcggcgttc
    39601 ttcgcctcct gcgccttcag ctcgcgcagg tagtcgtcga ggcggtcgaa gctctcttcc
    39661 cagaagcggc ggtagtgctc cacccagtcc gcgacgtcgc gcaggcggcc cgcctcgagg
    39721 cggcacgggc gccactgcgc ctcccggccc cgggcgatga ggccggcgcg ctcgagcacc
    39781 ttcaggtgct tggagacggc cggaaggctc atgtcgaagg gctcggccag ctcggtgacg
    39841 cccgcctctc ctgacgcgag gcgcgcgagg atggcgcgcc gggtggggtc ggcgagcgcg
    39901 gagaacgtgg agctgagggc gtcgggtgcg ggcgtcatat tcggtgcggc gtacttaacc
    39961 gcctggttaa gttatggcga ccgagtcccg acgtcaagaa gattcttcgt tgccggccgt
    40021 gccgcacgcc gcggcgcggg cgagaagcaa ctcgcgctcc cggcggttcg cggcgagggc
    40081 ggcggccttc tcgaa
//
LOCUS       DAOMFY010000005        39678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_320_length_39678_cov_8.863085, whole genome shotgun sequence.
ACCESSION   DAOMFY010000005 DAOMFY010000000
VERSION     DAOMFY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 39678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_39678_cov_8.863085"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..647
                     /locus_tag="PLB01_01030"
     CDS             99..647
                     /locus_tag="PLB01_01030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQQ75911.1"
                     /translation="MSSNGGTWREEWRSASSGLVGGAPAAATFSALSRTWPEGQRVEI
                     APAVRSFFLSKKSSSSSLSSLLLGALLLLDYGDTIETLYDRRPRGTVRGYAHHQRLEG
                     EELYRWGGKADVTADVNFTDVAAWAAEAGFTVAPLETQGAFLSRHVRDAAARAAREPA
                     LAFLLDPRGAGTGFKALELRKG"
     gene            complement(644..2008)
                     /locus_tag="PLB01_01035"
     CDS             complement(644..2008)
                     /locus_tag="PLB01_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ75912.1"
                     /translation="MPTRRIIVLVAAAALSLFLSKNLFSSPLPDLLDAAPSRFATSEG
                     VRVHYKSLGDPAAKTAVVFVHGFACDMTSWRAQAPAFDGKARMLFVDLPGHGKSDRPA
                     VDYTMERFAAAVEAAMRDAGVERAFLVGHSMGTPVARQFGRKYPARTLGIVAVDGALR
                     SYFKDPAQVDAFVARFSGPDYEKTMSGFFESTFTPATPDAVKADVRKMAAGFPQPVAA
                     SAMKGMFDPAIWKDDAIKAPLLVLVAKGPMWSADYFAYVKTLNPDATVVEVAGAGHFL
                     MMEKPAEVNAALVAFAAKAGAIPVRAARPGRPADVSSPEALVAAVYDVISGPAGVKRD
                     WDRFRSLFADGARLIPTGIRPAGDFGPSVYDPDGYVRRAEPVFETTGFYEKGVASRTE
                     RFGHVAQVFSTNEARHEPKAPQPFLRGINSFQLLFDGARWWVVTIFWEAESETVRIPK
                     EYLP"
     gene            complement(2098..2703)
                     /locus_tag="PLB01_01040"
     CDS             complement(2098..2703)
                     /locus_tag="PLB01_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HQQ75913.1"
                     /translation="MMTAEEDDREPLEARVARAVREDVAISLYDPRWPTSFRLEKEHL
                     LACLPGDLILRIEHFGSTAVPGLSAKPIVDMLVEVTNLQATRERIVPILESQGYDYFW
                     RPTFGDDGPPFYAWFIKREPGTGVRTHHIHMVESDFAEHWDRLLFRDYLIEHPEVARE
                     YEALKLRLAAASPHDRVAYTRGKTEFIQRVTGDAKRHARRI"
     gene            complement(2723..3385)
                     /locus_tag="PLB01_01045"
     CDS             complement(2723..3385)
                     /locus_tag="PLB01_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HQQ75914.1"
                     /translation="MYLSLGLATAIGFYHAPLVQGDLGAIARALTRSDVRFRIGVVAD
                     VLSAALVVPLAVLLHELLKPVHKVRAALMAVLLLVAMPISFVVALDYVAAQWLLSGAP
                     AAASLARDQREAFGMLLLRLHAHGVLAVEIFWGLWLVPFGLLVWKSRFIPRALGVLLV
                     VAGGAYVAHSIVSLLLDAPRNVAYERITMLARAAGEFPIMLWLLVKGADDRPRPIRRT
                     PG"
     gene            complement(3481..4374)
                     /locus_tag="PLB01_01050"
     CDS             complement(3481..4374)
                     /locus_tag="PLB01_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ75915.1"
                     /translation="MRDAALRIAFLVTIVARGVAFAASPEAGAVEAAAGIRADMDKGD
                     VDAAIARGEAAVAADRGDAETWLWLGRAYGRKAQTGSVFSRVGLAKKCQAAFEKAVAL
                     DPRSVDAREDLVTYYLQAPSIMGGSKEKARGQAAELAKLDAVRGHIARGRILAGDKDF
                     AAAETEFAQALAADPASFRGAVALGNFYVTQKRWADARALWQRRLDSNAGDTMARYQL
                     ARVASDSGTDLEKGVEHLEAYLAVPAQPGHPTWADAHWRLGLLYEKLGRADDAKAEMR
                     EALKLVPGHLQARKDLERFQK"
     gene            complement(4660..5160)
                     /locus_tag="PLB01_01055"
     CDS             complement(4660..5160)
                     /locus_tag="PLB01_01055"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ75916.1"
                     /translation="MSTIEAFVAELRHEAPLTRKMLERVPPAAFDWKPHEKSRALGEI
                     AAHLANLPGLFLAPLNADGYDRLEYAAATGTVEEILATFDHNVARALEALGDLTEEQL
                     GGPWTYRYGEKVIFEAPRIVVIRGMALNHSIHHRGQLSVYLRLLNVPVPAVYGPTADE
                     SQAYPG"
     gene            5835..6842
                     /locus_tag="PLB01_01060"
     CDS             5835..6842
                     /locus_tag="PLB01_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75917.1"
                     /translation="MRIDANGVLLRAYGYSLWFPVFLEDGVESAPVDFASVVLRTPPG
                     FAAVFAGQRIREESSPGGWVSEWRAEGLDLFAAQCVARRFVVTRVGRAFLYHDADPAS
                     HVSAQRIAALTSKLEEAYRQRYRTDVPDAQVHVMQMPRYGDISSGNVVGLNAASWAGI
                     EKDSRARRTLAHELVHPFVATGTPKADRLFALAVEGFPSYLHLPVLADLEGEASIDDL
                     LRRIETTYMDRKSGKGSGRHAQPPEKPLLEIRPDEVGLYKDGFVLGDRALLFLNWLRR
                     RMGKERFTDFTRDLFRARPLTEVRFRATVLRFLPGAEGDLDRWLETNDFPERFRLSAG
                     P"
     gene            7078..7698
                     /locus_tag="PLB01_01065"
     CDS             7078..7698
                     /locus_tag="PLB01_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75918.1"
                     /translation="MSPAKQAGAPELLQIYRDSLRPGADDAYRKVEEDAARICAELGF
                     PNPHLALESLSGPKEVWWLNSFPSEDVRKRVASDYASNRPLVDALEGIAKRKEGLVAE
                     PVDQIWRYRPDLSGGAAWDLSGARFVVATVGRDAAGTGAVFEIPDGSRVVLRKARSRA
                     EAEAFAQAAGPGARLFAIRPEWGMPAKAWVAADPEFWRANPAVRQE"
     gene            complement(7752..8588)
                     /locus_tag="PLB01_01070"
     CDS             complement(7752..8588)
                     /locus_tag="PLB01_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ75919.1"
                     /translation="MGIRHHVPVDRGQFMSIREQGHGPAVVLLHGIPGSGASWDHVAD
                     LLATRCRVLVPDLLGFAASSRSADPETLHARGQAAALQEALHGLQIERATLVGHDFGG
                     PVALSLIARLPALATGLGLLSTNTFPDTPIPFPLSATTWPLVGGLAGGILFSPASFRM
                     MLRQGVGPRSGALDAARAVGDAEQARAIATIFSTSLRSLAELYAPIEHALRSVTVPSF
                     VAWGDRDPFFGVDQGRRTAKALTGARWALLEGAGHFLPEERPTEVASLIAELVDARGG
                     SS"
     gene            8890..9339
                     /locus_tag="PLB01_01075"
     CDS             8890..9339
                     /locus_tag="PLB01_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQQ75920.1"
                     /translation="MRSRFVLALFLAAAGCASTPPRSGSASLRQEVIEVERAFARTMA
                     NRDLRAFLTYISADAVFFSGGKTLRGPQEIGAWWKRYYEESDAPFSWEPQEVEVLVSG
                     TLALSSGPVRNPKGELVGTFTSIWRREGSGTWRIVFDKGNKACPSAP"
     gene            complement(9446..9799)
                     /locus_tag="PLB01_01080"
     CDS             complement(9446..9799)
                     /locus_tag="PLB01_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75921.1"
                     /translation="MSETSDPVEASLASLARSTRSAAPRPVPFAVVRILAERRRAEFR
                     SRRLLAFVALASAAPMLIVLVAWGLRPAAASGSLLAASVLALAVVPAIDGIGRLAGTR
                     VPVATSLGHGSPWPS"
     gene            complement(9796..10284)
                     /locus_tag="PLB01_01085"
     CDS             complement(9796..10284)
                     /locus_tag="PLB01_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQQ75922.1"
                     /translation="MNLDPDLDEFYREMHPRLWAFLVRTTREAALAEEIAQESFVRLL
                     ASRGAALPSGERRAYLFKIAENLVRDSGRRRARERTTSLDEAPEPAAPDPEEPMGRRA
                     AAALAALGERERKLLWLAHVEEWNHNEIAGLLGLAAGSVRVLLYRARRHFQQHLEKEE
                     PR"
     gene            complement(10281..10775)
                     /locus_tag="PLB01_01090"
     CDS             complement(10281..10775)
                     /locus_tag="PLB01_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75923.1"
                     /translation="MAPYEETLFILAVLSFGAFVAWLAARHALERARERDRRGRFVEA
                     QIEKFGEARDFVEFARSEAGLAWLRADSGETRARRGLLVLTVVGILAIALGAGLLVNA
                     ARLAGAADPNDALSRTNAAWWGTVLVAVGAGSLAASALIARIGRAWGLVPHVGTKERE
                     AGGE"
     gene            complement(10800..12110)
                     /locus_tag="PLB01_01095"
     CDS             complement(10800..12110)
                     /locus_tag="PLB01_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQQ75924.1"
                     /translation="MKLSTALSSAAVLVFQSVCVAQGPALDQARQQAVIEKTIELVVA
                     NYVYPERARAVETALRAKAKAGTYSAKMKPDDFLDALNQDMQAAARDKHLRVLNNPRI
                     VAQLKSEAAGAKDVSPEFLRMLRENNFRLRKAESLDGNIGYFKFDNFVELRFVKDALV
                     GAMNFLHSSSALVLDLTDNGGGASETSDFLLSYFLPEGTRTGESWSRTTNQTTVSTVT
                     RSPDVKQMLETPVYVLVSERTASAAEAVAYTLQQAKRAVIVGSRTKGLANAGQHFVID
                     DRLFVMVPTILNKNAVSGTNWEGAGIAPDIAVAPEKALNSVMANALKSLADGKKDKKE
                     KQRLLFMAQDYASLLSPQAPPAGFLEACTGEYEGDQRFVMKDGSLHFLKGEIDRKMNY
                     MSDHTFTAEGRKDYRIRFPFDAGRVARCEVLWFDDTSDTYKRTK"
     gene            complement(12234..13532)
                     /locus_tag="PLB01_01100"
     CDS             complement(12234..13532)
                     /locus_tag="PLB01_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQQ75925.1"
                     /translation="MNSTLAMIILAAVATVPAGGAEPLRRRADLGFAAGRFTPGEGLM
                     VARVAPGTAAARAGLLAGDRLLGIGARTFTTGLDADAGMAALRSGERVTLRLTRGGVA
                     LALPVVLEAMPLDDMPEVEFSYEEIRNPRSGERQRVIVSRPRGTATRLPAVFFIPWLS
                     CDSVEAAPGARGGIETLLYRIASESGLVLMRVEKPGVGDSEGVCSETDLDTELAGNRA
                     AFEALKRHPWVDPARVVAMGQSFAGGLLPLVAPREETHGYLFLNSWVRTWLERLLEFE
                     RRRLEAAGLSAGDVSTRMRRLAELYGLVLEGGLTPAEASRRKPELGPVWEDAPAHQYG
                     RPIRFLQQLQATNVSAAWEKVDRPTLAVFGEADLVMSRSDHERVVALVNRNRPGAARL
                     LTVPGMDHGLSAPLPGGGEGLPEVVATTVLNWLRRVAGSP"
     gene            complement(13589..14212)
                     /locus_tag="PLB01_01105"
     CDS             complement(13589..14212)
                     /locus_tag="PLB01_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HQQ75926.1"
                     /translation="MLATPADEPILDQEWRDFLNQQDFGQVIAPGPNGQIPVVVPAHY
                     CYDGDRTIELHLHRENPVWTAIRANDRVVFAVIDAHVYIPSTWNAEDPSAADWAPPTS
                     YYAAVQAICSAVIPDEPAGVAALLRRQMRRMQPEGGYASIEPGLTPYGRMLGGIRGLR
                     LEIDEVRAKFKFGGNRSHQHRARVAERLAARGTEADLRARSHLLRRC"
     gene            complement(14790..15590)
                     /locus_tag="PLB01_01110"
     CDS             complement(14790..15590)
                     /locus_tag="PLB01_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ75927.1"
                     /translation="MRAGVNRLTGKVALVTGASRGVGKGIALELMDAGATVYVTGRSV
                     DDMQYVSERGIGIECDHRRDSQVEAAFRRIADEQGRLDLLVNNVWGGYENMLENGEFT
                     WSRPFWRQPVWRWDSMFQAGVRAHYVASQLAARVMVSQRSGLIVNISYWAASKYVGNV
                     AYGVSKAATDKMTADMSRELSDHAVAVVSLYPGLVRTERVMEAAQWLDLSNSESPRFV
                     GRAVVALASDPKITERSGTVLVAARLAQEYGFLDIDGNAPSPLSAEDV"
     gene            complement(15623..16399)
                     /locus_tag="PLB01_01115"
     CDS             complement(15623..16399)
                     /locus_tag="PLB01_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ75928.1"
                     /translation="MDKGDLDAAIKRGEEAVAADRGDSETWLWLGRAYGRKAQTASVF
                     SKVGFAKKCRSAFEKAVALDPRSVDAREDLISYYLQAPGIVGGSKEKAREQAAELAKL
                     DAARGYIANGWILADDKDHDGAEAQFSKALEADPTGFRGAVALGNFYVAQKRWGDART
                     LWQRRLDSSAADAMARYQLARIAYFSGTDLEKGVEHLRAYLAVPAPPGRPTWADAHWR
                     LGLLYEKLGRLDDAKAEMREALKLVPGHLQAQKDLGRLQK"
     gene            16970..17623
                     /locus_tag="PLB01_01120"
     CDS             16970..17623
                     /locus_tag="PLB01_01120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQQ75929.1"
                     /translation="MKLSDSRALSALELLLGTGVVIGHNVFRILPNEVPILVVLGLIS
                     FRVRSGGWAALPFRRPKSWRRLVSIAVAAAALRIVLSGFVESLASKVWPPIVAPAGSE
                     AIAGDLKTALMWLGLVWTWAAFGEEFGYRGYLLTRAADIGRGSGAAYLAGMVFVAVLF
                     GFGHFYKGPAGIVDSGFGGLVFGAAYLVSGRNLWASVLAHGFVDTYGITALYFGWAT"
     gene            complement(17664..18308)
                     /locus_tag="PLB01_01125"
     CDS             complement(17664..18308)
                     /locus_tag="PLB01_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ75930.1"
                     /translation="MIRVVLVEDQTLVRQGLRSLLELWGEVEVAGEASTGDEALRLIP
                     AVKPDVVLLDMRLPGPSGLDVLRGLAAAGFLPPTLVLTTFDDEALLLEAVRAGARGYL
                     LKDVTFDQLTDAIRRLARGETFFQPAVTRQAAASLEKSPAHTDGFETPEALTARERET
                     LRLMAGGYSNREIASAMGLSEGTVKNHVSSVLLKLGVRDRTRAVLKGLAEGLIG"
     gene            complement(18305..19456)
                     /locus_tag="PLB01_01130"
     CDS             complement(18305..19456)
                     /locus_tag="PLB01_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQQ75931.1"
                     /translation="MPDAPTSLATLRIAGLLAWGAAGLPLIRLLLRPRAGPPDPVIAL
                     GSAVLLAFGLAFFAATGGPGARRNRAAATALLAVQTAAALLFVFLVDSAAAGILLVIV
                     TAQLPFCLSPRAALLWCGTQNAVFAVLLVTKSGALNGGAVFAAFLAFQLFAFYTVSIA
                     EREARQRSELARLNTELVSAQKLLQESSRAAERLRISRDLHDALGHHLAALSLQLEVA
                     GQIADGRVREPVERARSLARLLLADVRTVVGGFREDDRVSLSDALRLLASDIPRPKIH
                     LDIAGDGLAADPAVTATLLRSVQEIVTNAVKHSGAENLWIAVTRTPYGLEVLAQDDGA
                     GAARIADGRGLSGLAERISEARGTFAVTTSPGRGFQVAIRIPCAPGGGS"
     gene            19573..20742
                     /locus_tag="PLB01_01135"
     CDS             19573..20742
                     /locus_tag="PLB01_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQQ75932.1"
                     /translation="MNRRTFLELLGAGGAAALLTAPGGGCAAKPLGALRPWEGPPGPP
                     GDGDPRLRLASWALLAPNAHNRQPWLVDLRGDGMDLFVDPRRLLPETDPASRQIMISQ
                     GTFLELLAIAATHEGLLARIELFPEGMVPAGDVGQRPVARVTLTPQRDLRKDELFPFI
                     RQRHTNRRPYADPAPTDREVADLAASAEGAGIRFRCLREAPALSRAAALMTEAMRIET
                     ATKRMHAETVAMLRFDDAETEQLRDGITLPNLGLTGFRLLVARSFVSRERAFSPDFLK
                     KTVDATRAQAFSAPAMGLLSTAGRSRADEVNVGRAFARLHLTATKLGLSLHPMSQVLE
                     IESERTRFVTEVLGSPAEEPQMLFRLGRSAPTPHSPRRDVAACLRTEGNFRRTDS"
     gene            20775..21335
                     /locus_tag="PLB01_01140"
     CDS             20775..21335
                     /locus_tag="PLB01_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75933.1"
                     /translation="MFLVWSVSGVSAILAVACVGLLRRDFRTAPRSWRWLAVSGLPLA
                     LAGLGFILRFWRPLPGPYLANESYPLGPYVNTWAVSFGFMWLAYGLAFFALAIRMPRD
                     ARSWAVLLVTWILAWLPHFLIGVGFAVAGSNRPSVGIYRAWASSWPGLFVLAGSAIAM
                     LLHFVFAACGFSLAALQIRRAEGDGR"
     gene            complement(21332..22084)
                     /locus_tag="PLB01_01145"
     CDS             complement(21332..22084)
                     /locus_tag="PLB01_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQQ75934.1"
                     /translation="MSATLRCAVVEDEPEARQNVVDYLEHISGVTVVGVASDGEAAVR
                     LVDDEKPDVLLLDVQLPEMDGFEVLRRIRHRPEVVFTTAFDDYAVSAFELGALDYLVK
                     PFGPARLEAAIERARVRMAARALDPAFAGPVERALAVTRHPLRRLFARTGGRIVPIEV
                     DDILRISASGEYSEVFTEKSSYLVVSTLSELVECLDPAVFERVHRSHIVNFRAVRHLR
                     AADDRRLLITLKDGTTVLASRAASVRLRRRVV"
     gene            complement(22081..23232)
                     /locus_tag="PLB01_01150"
     CDS             complement(22081..23232)
                     /locus_tag="PLB01_01150"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQQ75935.1"
                     /translation="MEIRDVHAAAAPRALEDPVSGGSATAVRIGWIAAYLLYLLVFAV
                     TFGLARGFSPLDALLVALVNTGPEAAGAPFVLRETARDGRHRFPTGLRLTLLGATFIA
                     WCVAGTLCGFIVWRTVGHGSWPVPTDPRNIAWKCLFSLLVFGALAGVGRARFHARRAA
                     EAAERAHRAEMLRLEARLSILRAQLNPHFILNVLHSLVGLARRDPDATSSALERLGTT
                     LRYALRVQNRRCDSVTLRDELAFTLEYLGLERLRLGERLSVRVRAQQGALDRTVPPFV
                     LQPLVENAVLHSISARARGGVVSIGIGTEGAALLVTVDDDGVPSPAAAPPRRPGSGLG
                     LKLLRDRLDAHYEGRGTLDVGPSPLGGTRARLRLEGEPAGAGTGDGEGE"
     gene            23509..23691
                     /locus_tag="PLB01_01155"
     CDS             23509..23691
                     /locus_tag="PLB01_01155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQQ75936.1"
                     /translation="MLFGLGHYDRGPVGIVDSAFAGLVLGAAYLISGRNLWASVLAHG
                     FIDTFGVFALYLGWSS"
     gene            23726..24460
                     /locus_tag="PLB01_01160"
     CDS             23726..24460
                     /locus_tag="PLB01_01160"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQQ75937.1"
                     /translation="MILRRLVLPLLFASTAPGQIPDSFSNLRFFPKDIPKARLVEEMK
                     SFTLATGLRCDGCHVGGPGLDTIDFASDEKETKRTARAMLAMVRAINDDHVGRLGRAK
                     DVKVVCATCHHGVSKPALLEKLLAATVREKGLDAAVATYRELRRTYYGRAAYDFGEAT
                     LNTAALSLIAEGKAKEAVRLVELNLEFGAREALTYNVLSDARLTAGDRPGAIAALKTA
                     VEMDPKADFLRKKLERLEKAAGRVDG"
     gene            24511..25527
                     /locus_tag="PLB01_01165"
     CDS             24511..25527
                     /locus_tag="PLB01_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HQQ75938.1"
                     /translation="MAGVWRGGQCRPPDFPRSPALHARLERTLTGHAAVVGQVAFSAD
                     GQFLASGSADRTVRLWRWREGVLARTLTNPEGVTSVAFSPDGNWLACGSYDRTVNLWR
                     LRDGMLAQTLRGHEGTVWSVAFSPDGQRLASSGEDRTVRLWRVSDGAPLRTLSGHGLN
                     VWSVAFSPDGRFLASASFDRTARVWRSDDGSLVRTLVGHSQAIVSLVFSLDSELLATG
                     GDDSSVRLWRANDGALVSTLTGCSEHVYAVAFSPDGRFLASGGRERGNLGTLWKKVMG
                     ERLSGGKGPTIRLWQVRDGVLQQALAEHSDDVRSIAFSPDGQWLASGGEDKTVILWRL
                     EAQP"
     gene            25810..26691
                     /locus_tag="PLB01_01170"
     CDS             25810..26691
                     /locus_tag="PLB01_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75939.1"
                     /translation="MRHALSFLAVALAAAPLAAAPPRTAASSNRALATAAADILRNDY
                     RADRAELFRLAQALESTAAAGPNEKYRRYWIAFAYWRRGFNGLNETPKPADLGEDFVR
                     CEAHARAALALDPAFEDARGALMGCLMGRLWAGAVPDDRKAEWIRQGTEVMQSLMKNA
                     GSNPRSLWLVGMQQAYRPAGGDTNVAVATWKKGLAGAREEALAAASRDPWVPSWGAPE
                     ILMNLANFHTYTKPDKAVARAYAEGALAMSPEWHYVRDILMPAIEKLPDPPAAAAATP
                     PPGRPSAATPAPTGGRP"
     gene            26688..27212
                     /locus_tag="PLB01_01175"
     CDS             26688..27212
                     /locus_tag="PLB01_01175"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ75940.1"
                     /translation="MNLREILAPEWRIEAATTRRVLERLPVDRLDFRPHAASRTLGEL
                     AAHVAVIPELFLARLPEEGLSIDGDAAPPSDPAGLLERFDRSSVRALEALESLSDERF
                     LEPWTYRRGDRVVFTLPRLIVARTTGLNHIVHHRGQLTVYLRILGVPVPSVYGPSADE
                     RVVGPRSVSEREPK"
     gene            27209..27787
                     /locus_tag="PLB01_01180"
     CDS             27209..27787
                     /locus_tag="PLB01_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75941.1"
                     /translation="MTLFDLIFIGVFLVTVTILLATAVAAVRGNRPRALGLLRGLGGL
                     IGVYLAVVIVVSLVSPRRLIPMKEPRCFDDWCVSVDRVDRTPAGTEVSYAVTLRLSSR
                     ARARAQRENGVSVYVLDDRGRRFEPREDPQDVPFNVLLHPGESVDATRRFVLPADVQG
                     PGLVIAHERFPGIFIIGDDQSMFHKPSVTRFP"
     gene            complement(27845..28639)
                     /locus_tag="PLB01_01185"
     CDS             complement(27845..28639)
                     /locus_tag="PLB01_01185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ75942.1"
                     /translation="MTKTFRPSDGPGSEALPGRLYTDLAPWWHLLAAPAEYAEAAAFY
                     WNVIRSACSSPPRSLLELGSGGGNTASFLKSHCTLTLVDLSPEMLGQSQSINPECEHL
                     SGDMRSIRLGRLFDVVFVQDAISYMRTEAELEQAIHSAYVHCRPGGVALFAPDYVREN
                     FRSSTSHGGHDGNGRAMRYLEWTWDPDPDDSTYLVDFAYLLRDEDGALRVESDRLQLG
                     VFRRADWLRIIGAAGFEPRAVPFEQSDVGPGSEVFLGLRPHEPKHN"
     gene            28763..29674
                     /locus_tag="PLB01_01190"
     CDS             28763..29674
                     /locus_tag="PLB01_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ75943.1"
                     /translation="MQKTFVLAAFAAWLALPASAQQDFSKVEIKAEKVAGGLFMLTGA
                     GGNIGVSAGPDGVFLVDDQYAPLTGKIKAAVAAISDKPIRFILNTHYHGDHVGGNENL
                     GKDGAVIVAHENVRKRMSTDQFLRLFNRTIPASPAGALPLVTFAESVSFHVNGEDVDA
                     VHLASAHTDGDVVVYFRKANVIHAGDNLFIGRYPICDISSGGSVEGMIGAADRLLAAG
                     DERTKYIPGHGPLGTKADVKAYRDMLAASRDAILPLVKAGKTLDEVKAARPTAALDEK
                     WGKPDVWTMVVYYSYGGKEPATPAPAK"
     gene            complement(29693..30751)
                     /locus_tag="PLB01_01195"
     CDS             complement(29693..30751)
                     /locus_tag="PLB01_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ75944.1"
                     /translation="MTAAKRIGLGVAAWIVALSLVHAAVNVNWAVLANDFLAPEKRKL
                     NVAYIPVTCHLACPVTDYISQFSESGELFIPRMFQGFPEIKEALIANKMQAAFIVAPM
                     AIALKAQGVGIRIVYLGHRYGSAVVVRKDGPVKTFPDLKGRTIAVPSRFSDERLIVFR
                     VLKKFGMKPEDLKIVEMAPPDVTGALAAGAIDAFSMGEPFPSQAEIGGYGRVLFHARE
                     HWPDYMSCVLVVREDVIAARPRTVQTLVDGIARSGLWLEDGKPNRDHAADFVGRFYYN
                     QDPKVLRWALTNPLDRVMYTPLSPRKADFDLVRDLMIETGVLDRRIEFDEYVDTRFAE
                     GAKEQTAWRYEPGSSEAR"
     gene            complement(30748..31524)
                     /locus_tag="PLB01_01200"
     CDS             complement(30748..31524)
                     /locus_tag="PLB01_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ75945.1"
                     /translation="MPTRSDLERVLLPVGTAALAVLLWALAVRLSHTNVFPSPLKVLS
                     GLQELAGRGVLWRYVGDSLRRVASGYFLALVLGIPAGLALGWHPNLASAFNPVIQMAR
                     PISPLAWMPLAILWFGVSEAAPGFLIFLGAFFPIVVAAMNGVRSVPAMYLQAGRNFGL
                     STPRLLARVVFPAALPQILVGLRIALGIAWIVVVAAEMIAVDSGLGYLIIDSRNAGKR
                     YDLVVAGMLLIGVIGLLLDTLARRLERLRTVRWGFQGEAA"
     gene            complement(31508..32266)
                     /locus_tag="PLB01_01205"
     CDS             complement(31508..32266)
                     /locus_tag="PLB01_01205"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ75946.1"
                     /translation="MTASPKVSLRNVSRSFATDGAPIDVLSSVSLDVADGEFVCILGP
                     SGCGKSTLLNIVGGFLKASAGSVLIDGEDVSGPDPRRIFVFQERGVFPWLTVSGNVGF
                     GLFALGAKERAQRVAHYVKLVGLEGFENAYPQELSGGMKQRLEVARALAVNPDVLFLD
                     EPFGALDSITRLVMRSELLRIRRAEKKTILFVTHDIDESVQLADRVVVMSTRPATIRR
                     IVDIEMPHPRDLSSARYIELRDLLLGEIGLAHQV"
     gene            complement(32263..33009)
                     /locus_tag="PLB01_01210"
     CDS             complement(32263..33009)
                     /locus_tag="PLB01_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ75947.1"
                     /translation="MKRLLPFTAAAALLGLWAVAVRFQTSGLFPDPLQVLSGLGELLQ
                     KGLLAKYVVASLFRVTWGYSLAVVTAIPLGLLLGWYRRGEQALNPILQLLRPISPLAW
                     IPISILWFGVGDVAAIFLIYIASALTLVVAAMNAVRNVPPALLRAGRNFGLSDRRLLL
                     DVVLPAALPQLIVALRISLGVAWLVVVAAEMIAVNSGLGFLIVDARNAGNRYDLVVAG
                     MVMIGLIGLALDVAIRRLETLKALRWGYGS"
     gene            complement(33013..34635)
                     /locus_tag="PLB01_01215"
     CDS             complement(33013..34635)
                     /locus_tag="PLB01_01215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQQ75948.1"
                     /translation="MQEIPSQESLAFDVVVVGGAISGASTAVLLRRRMPDVKVLVVEK
                     SEAFDWKVGESTVEISAYFLTRELKLYDHLSREQLPKQAFRYWFHNENVSCLREASET
                     GPTQLARTPSFQIDRQKLDEHVLALAAKEGATVWRPAKVVDVALNGEAGNTLVVEKAD
                     GSRQTVSCKWVVDATGRQAMLARKRGGLTPIESHPTSAIWVRYRGVLDMDGPEFLTGD
                     ASDPFHRAVLTARRLATNHFTGWGYWIWFIPLQGGETSVGLVWDTRLVQPEGATPLEK
                     LTNFLNANPLTRELLAKAEPVADDCRMYGHLPYFVDTFVGPGWTCVGDAGGFLDPFYS
                     PGLDQMAFSVYSRVGLIQKSLAGAPPEEMAKEYELHNTRYARFFRYFYEAIFRDKYHV
                     MGDYDTMTTTFLMDTALYYLAAVIPTYRWSSERLGLPPYYQDGSEIAFYPMRFYNRRL
                     VAIAKRKKALGIYGNHNAGKRPGFVGFSVRSAAWVMLAHGVLRWARAELANAWTYVVK
                     PRTVGGPVPILPPRATRAVPAPGVPESAPARN"
     gene            complement(34645..36885)
                     /locus_tag="PLB01_01220"
     CDS             complement(34645..36885)
                     /locus_tag="PLB01_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQQ75949.1"
                     /translation="MSRRRIALLAALAALGWWFYENASLPRLRATFVPWMHRLERYRD
                     ALETTRAALRAQSAHPGDAEGIAALRAWAWKDLDRVAMVSFDVDGKPEIERKKTGEKI
                     FLEGEERWELRGTATFVVDGFRADGARVERAARVTLLLPDANAPLPSPLTFKEIPSSS
                     FFSLSFSPLGGDRVETRPRFHDATLAAGLGAPRRDPPQRLTNRLIDGIWPGSGVAVLD
                     FDGDGAEDLFVGDGVRSILYRNDGHGRFTDVTVRAGLAKADGSGVPATGVAAGDVDGD
                     GFPDLAVTDAFGPTRLFRNRGDGTFEEITASSGVVTAGPTRSLAFADVNGDGSLDLFV
                     SGTGDYYGQMPDPPFDANDGRRNYLFLGDGKGHFRDASEAWGVAKPTRWSLSCLFADF
                     DGDGRPDLVVTNDFGLKNLYRNVDGRRFEDVTAKLGAEDRGYGMSAAWGDFDGDGRMD
                     LYTTGTYTQWGFLHEYPGLPVPLPGRIFRPIAISWMEKMCRGNSLLLQTAKGRFEDAT
                     ARSGAGRAGWNWSAIAADLDDDGRLDVYATNGMWGDGRVHDRELEFWWETLAYWDDYI
                     AKTKTFDRQGAGVQGVERDAYFVNRAPGPTAFEERGFLDGLDLETNGRAAVAFDANSD
                     GALDVYVRSVQAPEALFLGSRREPDAEHFLRLRLKGTRGKDNADGLGVRVSATLADGR
                     VLVRESGYASGYLSTGSPIVHLGLGRASRVERLVLRWPSGFVQDLGSVATVDRTLVVD
                     EGHGIR"
     gene            complement(36892..38517)
                     /locus_tag="PLB01_01225"
     CDS             complement(36892..38517)
                     /locus_tag="PLB01_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQQ75950.1"
                     /translation="MTDDVRTLAAEVARRRTFAIISHPDAGKTTLTEKLLLYGGAIHL
                     AGSVKTRKNQRHTTSDWMELERQRGISITTSVLQFPYQGHHVNLLDTPGHNDFSEDTY
                     RTLAAADAAVMLIDSAKGVEPQTIKLFEVCRMRRIPIVTFVNKMDRAGKDPLDLMDEI
                     ERVLGIPCHPVTWPIGSGPDFQGVYDRWGKTLLRYGRGDFSAKGERVAPVGVTSLDDP
                     GFVVSLGETAHATLRGELELLEAGHAFERATFLAGGITPVFFGSAVNDFGVEPFLQRF
                     LDLAPPPLPRASSGGLVDPLLPRFSGFVFKIQANMDPAHRDRVAFVRVVSGRFTRGME
                     ATHARTGKILKLAKPLSFLAQERTLVEEAWAGDILGLWDPGNLRIGDSLAEGAAIEYE
                     GIPRFTPEHFNRVVMLDPAKRKQLKKGLDQLSEEGAVQVFHDPDRMERDPVLGAVGVL
                     QFEVIQHRLRAEYGAEISYARLPFTLARWVEGEGFDPKRFDDPPSVASLVDLEGRPLV
                     LFQSPWHLERAQRENPKLTFVAAVQPGRAKAST"
     gene            complement(38519..>39678)
                     /locus_tag="PLB01_01230"
     CDS             complement(38519..>39678)
                     /locus_tag="PLB01_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQQ75951.1"
                     /translation="DPAKPARAAWKEIVPERRDAALQSFHVVGGKLALDYLKSASSLL
                     EIRNLDGTLLREVPLPGIGSASNLSGRDDEDEAYFSFTSFTTPTSIYKTSVKSGATSL
                     YFQVKVPADVSPYTVTQVFYPSKDGTRVSMFIVHRKDFHRDGTARAMLEGYGGFLVSF
                     TPNFSGTLFPWLERGGIYAVPNLRGGGEYGEEWHEAGMLLKKQNVFDDFAAAAAYLAR
                     EKWTSPERLVLRGGSNGGLLLGAMVAQHPGLFRVALCGVPLLDMVRYHLFGSGKTWIS
                     EYGSADDAAQFKALHAYSPYHHLRAGTKYPSVLLLSADSDDRVDPMHARKFAAALQAA
                     STGGPVLLRIEKHAGHGGADLVKAAVEERADEYAFALSETPGAIPAPSVNH"
BASE COUNT         6225 a        13494 c        13788 g         6171 t
ORIGIN      
        1 gggaagagag ggaagagagg gaagagaggg aagagaggga agagagggaa gagagggaag
       61 agagggaaga gagggaagag agagaaggga gaccggggat ttcttcgaat gggggtacgt
      121 ggagggagga gtggcggagc gcgtcgagcg gactcgtcgg gggcgcgccc gcggctgcca
      181 ccttcagcgc cctctccagg acatggcccg aagggcaacg cgtcgagatc gctcccgccg
      241 tccgctcttt cttcctttca aagaaatctt cgtcttcctc tctctcttct cttctccttg
      301 gtgctcttct cttgctcgat tacggcgaca cgatcgagac gctctacgat cggaggcccc
      361 ggggcacggt gcgcgggtac gcgcaccacc agcggctgga gggagaggag ctctaccgct
      421 ggggcgggaa ggccgacgtc acggccgacg tgaacttcac ggacgtggcg gcctgggccg
      481 ccgaagcggg attcacggtc gcgccgctgg agacgcaggg cgcgttcctt tcgcggcacg
      541 ttcgggacgc cgccgcgcgc gcggcgcggg agcctgcgct cgcgttcctg ctcgacccgc
      601 gcggcgccgg gacgggcttc aaggcgctgg agctgcggaa gggctagggc aggtattcct
      661 tcgggatcct gaccgtctcc gactccgctt cccagaagat ggtcacgacc caccagcgcg
      721 ccccgtcgaa gagaagctgg aacgagttga tgccgcggag gaacggctgc ggggccttcg
      781 gttcgtgccg cgcctcgttc gtcgagaaga cctgcgcgac gtgcccgaac cgctcggtgc
      841 ggctcgcgac ccccttctcg tagaagcccg tcgtctcgaa cacgggctcg gccctccgga
      901 cgtagccgtc cgggtcatag acggacggcc cgaagtcgcc cgccggccgg atgcccgtcg
      961 ggatcagccg cgcgccgtcc gcgaagagag agcgaaagcg gtcccagtcg cgcttcacgc
     1021 ccgcgggccc cgagatcacg tcatagacgg ccgcgacgag cgcctccggc gaggagacgt
     1081 cggcgggccg gccgggccgc gccgcgcgca ccgggatcgc ccccgccttc gccgcgaacg
     1141 cgacgagggc cgcgttcacc tcggcgggct tttccatcat caggaagtgg ccggcaccgg
     1201 cgacctcgac gaccgtcgcg tccgggttca gcgtcttcac gtaggcgaag tagtccgcgc
     1261 tccacatcgg gcccttcgcg acgaggacga gaagcggcgc cttgatcgcg tcgtccttcc
     1321 agatcgcggg gtcgaacatc cccttcatcg cgctcgcggc gacgggctgc gggaacccgg
     1381 cggccatctt ccggacgtcg gccttgaccg cgtccggcgt cgccggcgtg aacgtggact
     1441 cgaagaaccc gctcatggtt ttctcgtagt ccggtcccga gaaacgcgcg acgaacgcgt
     1501 cgacctgcgc ggggtccttg aagtacgacc tgagcgcgcc gtccacggcg acgatcccga
     1561 gcgtcctcgc cgggtacttc cgcccgaact gccgggccac gggcgtcccc atgctgtggc
     1621 cgacgaggaa cgcgcgctcg acgccggcgt cgcgcatcgc ggcctccacg gcggcggcga
     1681 agcgttccat cgtgtagtcg accgcgggcc ggtcgctctt cccgtggcct ggcaggtcca
     1741 cgaacagcat ccgcgccttg ccgtcgaacg cgggcgcctg cgcgcgccac gacgtcatgt
     1801 cgcacgcgaa gccgtggacg aagacgacgg ccgtcttcgc ggcggggtcg ccgaggctct
     1861 tgtagtggac cctgacgccc tcggacgtcg cgaagcgcga cggcgcggcg tcgaggagat
     1921 cggggagggg agaggaaaag agattctttg aaagaaagag ggagagggcg gcggccgcca
     1981 caagcacgat gatgcgtcgg gtgggcatcg tcatattcac cttccaggag aagcgaatca
     2041 aagcgtggaa cattatctgg ccgccgccgc tccggccccc tgttcgaggc gtcgctgtca
     2101 gattcggcga gcgtggcgct tggcatcccc ggtgacccgc tgaatgaact cggtcttgcc
     2161 gcgcgtgtac gccacccgat cgtgcggcga cgcggctgca agccggagct tcagggcctc
     2221 gtactccctg gccacttccg ggtgctcgat caggtagtca cggaagagga gtcgatccca
     2281 gtgctccgcg aagtcgctct cgaccatgtg gatatggtgc gtgcgcaccc cggtgccagg
     2341 ttctcgcttg atgaaccagg cgtagaaggg cggcccgtcg tctccgaacg tcggacgcca
     2401 gaagtagtcg tagccctggg actcgaggat cggaacgatc cgctccctcg tcgcctggag
     2461 gttggtcacc tccacgagca tgtccacgat cggttttgcg gatagccctg gaaccgcggt
     2521 actgccgaag tgctcgattc gcaggatcag gtcgcccgga aggcacgcta gcagatgctc
     2581 cttctccagc cggaacgagg tcggccaccg gggatcgtat agagagatcg caacgtcctc
     2641 gcggaccgct cgcgcgacgc gtgcttcgag cggctctctg tcatcttcct cggccgtcat
     2701 catcgtgttg ccactttcca attcagccgg gcgtacggcg gatcggccgc ggacgatcgt
     2761 ccgctccctt gacgaggagc cagagcatga tcggaaactc gccagcggcg cgggccagca
     2821 tcgtgatccg ctcgtaggcg acgttgcgcg gcgcgtccag cagcagcgag acgatgctgt
     2881 gcgcgacgta ggctccgccc gcgacgacga ggaggacgcc cagtgcgcgg ggaatgaaac
     2941 gcgacttcca gacgaggagg ccaaaaggca cgagccacag cccccagaag atctcgaccg
     3001 ccagcacgcc gtgcgcgtgc aggcggagca agagcatccc gaacgcctcc cgttgatcgc
     3061 gggcaagaga cgctgccgcc ggcgcgccgc tcagcagcca ttgggccgcg acgtagtcga
     3121 gcgcgacgac gaacgagatg ggcatcgcga cgaggagcag gacggccatc agcgcagccc
     3181 ggaccttgtg aaccggcttg agcagctcat ggagcagcac ggccagaggc accaccagcg
     3241 ccgccgagag gacgtcggcg accactccaa tgcggaagcg gacgtcggac ctcgtcaggg
     3301 cgcgggcgat ggcgccgagg tcgccctgga ccagaggtgc gtgatagaag ccgatggccg
     3361 tcgcgagccc gagggagagg tacacgaaac ccgccagtct cgcgagccgc ctcgtcgcct
     3421 ctggagatcg ctcttcaacg aggtccatct ggtcgctctc ccggctcgtg cgcgtcgtat
     3481 tcacttctgg aatcgctcga ggtccttccg cgcctgaagg tgtcccggga cgagcttcag
     3541 ggcctcgcgc atctcggcct ttgcgtcgtc cgcacgcccc agcttctcgt agagcaggcc
     3601 gagacgccag tgggcgtcgg cccacgtcgg gtggccgggc tgcgccggca cggcgaggta
     3661 cgcctcgaga tgctcgacgc ccttttccag gtcggttccg gagtcgctcg cgacccgggc
     3721 gagctggtag cgggccatcg tgtccccggc gttcgagtcg aggcgccgct gccagagggc
     3781 gcgggcgtcg gcccagcgct tctgcgtcac atagaagttc ccgagcgcca ccgccccgcg
     3841 aaagctggcg gggtcggccg cgagcgcctg ggcgaactca gtttcggcgg cggcgaagtc
     3901 cttgtcgccc gcgaggatcc ggcctctggc gatgtgcccg cgcacggcgt cgagcttcgc
     3961 gagctccgcc gcctgcccgc gggccttttc cttgctgcct cccatgatgc tgggggcctg
     4021 caggtagtag gtgacgaggt cctcgcgcgc gtcgacgctc cgaggatcga gcgcgaccgc
     4081 cttttcgaat gcggcctggc atttcttcgc gaggccgacc ctcgagaaga ctgaccccgt
     4141 ctgcgccttg cggccgtagg cacgcccgag ccagagccac gtctcggcgt cgccccggtc
     4201 cgccgcgacg gccgcctcgc ctctcgcgat cgcggcgtct acgtcaccct tgtccatgtc
     4261 ggcgcgaatg ccggcggctg cctcgaccgc gccggcctca ggagaggccg cgaaggcgac
     4321 cccgcgggcg acgatcgtga cgaggaaggc gattcgaagt gcggcgtctc tcattctctc
     4381 cttccgtcgg caggggaaga gggcgcctca cctgtctcac caccgaaccg caagacccac
     4441 cgaccgctac agcgtgggtc ccgacctcgc tccaagcgag cccggaaccc ttcaactgcc
     4501 gttcccggcc atccaatggc ggacgatcgc cttgagacgg cgcagacggg tgcgcgacgc
     4561 tcgcccgata tccgcggcac ctcgtcgtgg agcgcgctct cgagtcccgt ttcggagagt
     4621 cgttccgcgc ctaccgctcc gaagtgccga ggtgcggctt cagcctgggt aagcctggga
     4681 ttcgtccgcc gtcggcccgt agaccgccgg gaccggcacg ttcagcagcc gcaggtacac
     4741 ggaaagctgg ccgcgatgat ggatcgagtg attcaaggcc atcccgcgga tgacgacgat
     4801 gcggggcgcc tcgaagatca ctttttcgcc gtaccggtag gtccacggcc cgccgagctg
     4861 ctcctccgtc aaatcgccca gcgcttccag cgcgcgagcg acgttgtggt cgaacgtcgc
     4921 gaggatctcc tccacggtgc ccgtcgcggc cgcgtactcc aggcggtcgt aaccgtcggc
     4981 gttcaaaggt gcaaggaaca ggcccggcag gttggcgagg tgtgcggcga tttcaccgag
     5041 cgctcgcgac ttctcgtgcg gtttccaatc gaacgccgcg ggcggcacgc gttccagcat
     5101 tttgcgcgtc aatggcgcct cgtgacggag ttccgcgaca aaggcctcga tggtgctcat
     5161 ctccgactat ttcccttctc ttgatcgttt gcccgtctct ggccgctggc ccgcgcatat
     5221 cgagacgccc gccctcggcg gcgccatcgc caggaattct gtggccaacc gagattcaag
     5281 gccgacagat tccgtgcgaa ctgccgaacc aggtcggcga gcggcgtcgt taccctgtcc
     5341 gccggtgcag agtcgagaag ggagaacctc cgcatgagca cattcgtaca tgttccagtg
     5401 caagtgttgg agggttcgag atgtcgggta acacagccct tcggtgtctc gctctggtct
     5461 ttgccctgtg cacgtccctc gacgctgcgg ccggcgacgg cccgatgccg gccgtctcga
     5521 gctacaggat cgacgccacg ttcgttccaa cctccggaac gatggaagcg acggcggagg
     5581 ttcgattccg ggaaggagcg ccgctcccag atccgctcgt cttctatctc catggcgaac
     5641 tccaggtgtc ctccgttcgt gcagcgggct cggagatgga gttctcgggg cggccggtcc
     5701 tctacaccta cgattactcc ctcgttgcga cacgcgtcga agtggcgcga aagggagccc
     5761 cgctcagaga cggcctgacg gttcactatg cgggatcatt caacccgtcc cgggcccggt
     5821 ccgaatcgga ctacatgaga atcgacgcga acggagtgct cctccgcgcg tacgggtact
     5881 cgctgtggtt tccggtgttt ctcgaggacg gagtcgaaag cgcgcctgtg gatttcgcgt
     5941 ccgtcgtgct tcgcacgccg cccggattcg ccgcggtgtt tgcgggtcaa cggattcgcg
     6001 aagaatcgtc tccgggcggc tgggtcagcg agtggcgcgc cgaggggctc gacctcttcg
     6061 cggcacagtg tgtcgcacgg cgcttcgtcg tgacgagagt cggccgcgca ttcctgtatc
     6121 acgatgccga tccggcttcg cacgtttccg cccagcgaat cgcggcattg acgtcgaagc
     6181 tcgaagaagc ctaccggcag cggtatcgga cggatgtccc ggatgcccag gtgcacgtga
     6241 tgcagatgcc tcgatacgga gacatctcga gcggcaacgt cgtcggactc aacgccgcga
     6301 gctgggcggg catcgaaaaa gacagtcgtg cgcgacggac gctcgcacac gagctcgtgc
     6361 atccctttgt cgcgaccggc actccgaagg ccgaccggct ctttgccctc gcggtcgagg
     6421 gtttccccag ctacctgcat ctgccggtac tcgccgacct cgagggggag gcgtccatag
     6481 atgacctgct ccgccgcatc gagacgacgt acatggaccg aaagagcggc aagggctcgg
     6541 gccggcatgc ccagccaccc gagaagcctc tccttgaaat ccgtccagac gaggtggggc
     6601 tctataagga cggattcgtc cttggagacc gcgctctgct gttcctgaac tggctgcgcc
     6661 gccgcatggg gaaggagcgc tttaccgact tcacgcgcga cctttttcgc gcaaggccgc
     6721 tgactgaagt ccgcttccgc gcgaccgtcc ttcgatttct ccccggcgcc gaaggcgacc
     6781 tcgatcgatg gctcgagaca aacgatttcc cggagcgatt ccgactctca gccggtcctt
     6841 gacgaggccg ccgctcgcac cctcgaaacg gcagttcgcg ggagttccgc cgcgtcgaac
     6901 ccggtcccgg ggcgtaggtt gtctctcagg agcatttcga tggacgcaat taaccgacgc
     6961 cgcctaggca cggctgcggc cttcttcgcg gcgttcacgg ccgcgggtct cgccgtccgg
     7021 tggccgggcg gcgggtctgc gaagcgcaag gcagatcacc ctgcttcggg agcgaccgtg
     7081 tcgccggcga aacaggccgg ggcgccagag ctccttcaga tctaccggga ttcgctgcgg
     7141 cccggagcgg acgacgcgta ccggaaggtg gaggaggacg cggcgcggat ttgcgcggag
     7201 ctgggattcc cgaacccgca tctcgccctc gagtcgcttt cgggcccgaa ggaggtctgg
     7261 tggctgaact ctttcccgtc ggaggacgtg cgaaaacgcg tggcctccga ctacgcttca
     7321 aaccgcccgc tcgtggatgc actggaggga atcgcgaagc gtaaagaagg cctcgtcgcg
     7381 gagcccgtcg accagatctg gcgctaccgg ccggacctca gcggcggcgc cgcctgggat
     7441 ctgtcgggcg cgcgcttcgt cgtcgccacg gtcgggaggg atgccgccgg cacgggtgcg
     7501 gtcttcgaga tcccggatgg cagccgcgtc gtgctgcgga aggctcgttc gcgcgcggag
     7561 gccgaagcgt tcgcccaagc ggcggggccg ggggcgaggc tcttcgcgat tcgccccgag
     7621 tgggggatgc cggcgaaggc gtgggtcgcc gcggacccgg agttctggag ggcgaatccc
     7681 gccgtgcgcc aggagtgaga ggcgcgttcc cgcatgcgct cgagttcatc gcagcgcgtg
     7741 atgcgacgtc ctcacgatga gccgccgcgc gcatcgacca gctcggcgat gagcgaagcg
     7801 acctccgtcg ggcgttcttc aggaagaaaa tggcccgcgc cctcgagcag agcccagcgc
     7861 gcgccggtga gcgccttcgc ggtgcggcgg ccctgatcca cgccgaagaa cgggtcacga
     7921 tcgccccagg cgacgaacga cggcaccgtg acggaccgaa gcgcgtgctc gatgggcgcg
     7981 tagagttccg cgaggctgcg gagagaggtc gagaagatcg tcgcaatcgc tcgcgcctgc
     8041 tccgcgtcgc cgaccgcgcg cgccgcatcg agagcgccgc tgcggggacc cacgccttgc
     8101 cgcagcatca tccggaacga cgcgggcgag aacaggattc cacccgcgag cccccccacg
     8161 agcggccacg tcgtggccga cagcgggaag ggaatcggcg tgtcggggaa cgtgttggtc
     8221 gagagcagtc ccagcccggt cgcgagcgcc ggcagccggg cgatcaatga gagcgccacc
     8281 ggcccgccga agtcgtgacc gacgagcgtg gcccgctcga tctggaggcc gtggagcgcc
     8341 tcctgcagcg cggccgcctg cccgcgcgcg tgcagcgtct ccggatccgc gcttcgcgat
     8401 gacgcggcga acccgagcaa atcgggaacc agcacccggc agcgtgttgc cagcaggtct
     8461 gccacgtgat cccaggacgc ccccgatccc ggaatgccgt gcagtagaac gacagccggg
     8521 ccgtgcccct gctcacgaat gctcatgaac tggccccggt cgacggggac gtgatgccgg
     8581 atgcccatgc cgtccttacc tcccaaggtc gctgatgact ggagagaccg cgtccccgag
     8641 gacgctcgcc cgacgacctc ggggcgattc ctcacgcctt ccacgaccct ccgctcggta
     8701 ttgtgctgac gggccgggga gcccaagagg ggcaaacctg cgaactgcca atcgagctgg
     8761 ccgaacaggc cggcggcacc cgcgacggga gcggctccaa ccgagctctg cgggagaaac
     8821 ctctctcgca gttgtccgta gaggctcagc ggtgagtggg ctgaaatccc caagccagaa
     8881 gggacgatca tgaggtcacg attcgtcctc gcccttttcc tcgcggcggc aggttgcgcg
     8941 tccacgccac cccgtagcgg ctccgcttcc ctgcgccagg aggtgatcga ggtcgagcgc
     9001 gccttcgccc gtacgatggc gaatcgcgac ctcagggcct tcctgacgta catctcggct
     9061 gacgccgttt tcttctcggg cgggaagaca cttcgcgggc ctcaggaaat cggagcctgg
     9121 tggaagaggt actacgagga gtccgacgcc cctttctcat gggagccgca ggaggtggaa
     9181 gtcctcgtgt ctggcacgct ggcgctcagc agcggccccg tccggaaccc caagggagag
     9241 ctcgtcggca cgtttacgtc catttggagg cgagaagggt ccggcacgtg gcgcatcgtc
     9301 ttcgacaagg gaaacaaggc atgcccgtct gcgccctagc acctcgccct cgccgccgcc
     9361 cgcaggaact tcatgctcgt cgcgtcgatg cagcgggccg gtgtcctgat catcgcggaa
     9421 cgcttttgcg cacgacggcc ttctcctaac tcggccacgg cgatccgtgg ccgagggacg
     9481 tggccacggg caccctcgtg cccgcgagcc ggccgatccc gtcgatcgcc ggcacgaccg
     9541 ccaaggccag cacgctcgcg gcgagaagcg agccgctggc ggccgccggg cggaggcccc
     9601 aggcaacaag gacgatcagc atcggtgcgg cgctcgcaag cgcgacgaac gcgaggagac
     9661 ggcgcgaacg gaactccgcg cgccgacgct cggcgaggat ccggacgacg gcgaacggga
     9721 cgggacgcgg cgcggcggag cgcgtgctcc gggcgaggga cgcgagggaa gcttcgacgg
     9781 ggtcgctcgt ctcgctcatc ggggttcctc cttttcgaga tgctgctgga aatggcgtcg
     9841 agcccggtag aggagcacgc ggacgctgcc ggctgcgagg ccgaggagtc cggcgatctc
     9901 gttgtggttc cactcttcga cgtgggcgag ccagagaagc tttcgctccc gctcgccgag
     9961 ggcggcgagg gcggctgcgg cgcgccggcc catcggctct tccgggtccg gggccgccgg
    10021 ctctggcgcc tcgtcgagag acgtcgtcct ctcgcgagcc cggcggcggc ccgagtcccg
    10081 gaccaggttt tcggcgatct tgaacagata ggcgcggcgc tcgcccgagg ggagcgccgc
    10141 cccgcggctt gcgagcaggc ggacgaacga ctcctgggcg atctcctccg ccagggccgc
    10201 ctcgcgggtc gtccgcacga ggaaggccca gagccgcgga tgcatctcgc ggtagaactc
    10261 gtcgagatcc gggtcgaggt tcattccccg cccgcttcgc gctccttcgt accaacgtga
    10321 gggaccagcc cccaggcccg cccgatccgg gcgatcaggg ccgaggcggc aagcgacccc
    10381 gcgccgacag ctacgaggac ggttccccac caggcggcat tggtgcggga gagcgcgtcg
    10441 ttcgggtctg ccgcccccgc aaggcgcgcg gcgttgacga ggaggccggc accgagcgca
    10501 atggcaagga tcccgaccac ggtcaggacg agcaggcctc gccgcgcccg cgtctcgccg
    10561 gagtcggccc tcagccaggc gagccccgcc tcgctccggg caaactccac gaagtcccgg
    10621 gcctcgccga acttttcgat ctgggcctcg acgaaccggc cgcggcggtc ccgctcgcgg
    10681 gcccgctcga gagcgtggcg ggccgccagc cacgcgacga aagcgccgaa ggagaggacg
    10741 gccaggatga acagcgtttc ctcgtagggt gccatcggga gttctccctt ccgccttctc
    10801 tacttcgttc tcttgtacgt gtcggaggtg tcgtcgaacc acaggacttc gcaccgcgcg
    10861 acccgccctg cgtcgaacgg gaatctgatc ctgtagtcct ttcgtccctc ggcggtgaac
    10921 gtgtggtcgc tcatgtagtt cattttcctg tcgatctcgc ccttcaggaa gtggagcgaa
    10981 ccgtccttca tcacgaatct ctgatcgccc tcgtactccc cggtgcacgc ctcgaggaac
    11041 ccggccggcg gcgcctgggg agagagcagg gacgcgtaat cctgggccat gaagagcagg
    11101 cgctgcttct ccttcttgtc cttcttgccg tccgcgaggc tcttcagggc gttcgccatg
    11161 acgctgttga gagccttctc gggcgcgacc gcgatgtcgg gagcgatccc ggcgccttcc
    11221 cagttcgtcc cgctcacggc gttcttgttg aggatcgtcg gaaccatcac gaaaagccgg
    11281 tcgtcgatga cgaagtgctg gccggcgttc gccaacccct tcgtccgcga tccgacgatc
    11341 acggcacgct tcgcctgttg gagggtgtac gccaccgcct ccgcggccga cgccgtccgc
    11401 tcgctgacga gcacgtaaac aggggtttcg agcatctgct tcacgtcggg agagcgtgtc
    11461 acggtcgaga ccgtcgtctg attcgtcgtc cggctccacg attcgcccgt gcgggtgccc
    11521 tccggcagga aatagctcag caggaagtcg gacgtctccg acgctccgcc gccgttgtcc
    11581 gtcaggtcga gaacgagcgc cgaggagctg tgcaggaagt tcatcgctcc gacgagagcg
    11641 tccttgacga accgcagctc gacgaagttg tcgaacttga agtacccgat gttgccgtcg
    11701 agggactcgg ccttccggag cctgaagttg ttctcccgga gcatcctgag aaactccggg
    11761 gaaacgtcct tcgctccggc ggcctcgctc ttcagctggg cgacgatccg cgggttattg
    11821 aggaccctga ggtgcttgtc cctggcggcc gcctgcatgt cctggttcag ggcgtcgagg
    11881 aagtcgtccg gcttcatctt ggcgctatac gtgccggcct tggccttcgc ccgaagggcc
    11941 gtctcgacgg cccttgccct ctcggggtaa acgtagttgg cgacgacgag ctcgatcgtc
    12001 ttttcgatca cggcctgctg ccgggcctga tccagcgcgg gcccctgggc gacgcagacg
    12061 gactggaaga cgagaacggc ggcggacgaa agcgcggtcg agagcttcat gaatcctccg
    12121 aggcgccggg accgacgggc tccgggcctt cacggcgaga aacgccggga ggacggtttg
    12181 gttaacagcg cgcgatgtac cgacgatgcc gccggccttc gcaggcgaga acatcaaggc
    12241 gaaccagcca cccttctgag ccagttcagt actgttgtcg cgacgacttc cggaaggcct
    12301 tccccgcccc cggggagcgg tgccgacagg ccgtgatcca tcccgggtac cgtcaggagc
    12361 cgagcggcgc cgggccggtt ccggttcacg agagccacga ccctctcgtg gtcgcttcgg
    12421 ctcatcacga gatcggcttc tccgaagacc gcgagcgtgg gccgatcgac cttctcccac
    12481 gccgcgctca cgttcgtcgc ctggagctgc tggaggaaac ggatcggccg cccgtactga
    12541 tgcgccggtg catcctccca gacgggcccg agctccggct tcctccggct cgcctccgca
    12601 ggcgtcaggc cgccctccag cacgaggccg tagagctcgg cgaggcggcg catgcgggtt
    12661 gacacgtcac cggcggagag acccgcggcc tcgagccgcc gcctctcgaa ctcgaggagt
    12721 cgctcgagcc acgtccgcac ccagctgttc agaaacaggt atccgtgcgt ctcctcccgc
    12781 ggggccacga ggggcagcag gccgccggcg aagctctggc ccatcgcaac aacccgcgcc
    12841 gggtccaccc agggatgcct cttcagcgcc tcgaatgccg cccggttccc ggcgagctcc
    12901 gtgtcgaggt cggtctcgct gcacacgccc tcgctgtccc cgacgccggg tttctcgacc
    12961 ctcatgagga cgagacccga ctccgatgcg atgcggtaga ggagcgtctc gatcccgccc
    13021 cgcgctccgg gcgctgcctc gaccgaatca cagctcagcc aggggatgaa gaagaccgcc
    13081 gggagacgcg tcgcggttcc gcgcggccgc gagacgatga cgcgctggcg ttcgccggaa
    13141 cgcgggttcc ggatctcctc gtacgagaac tcgacttcgg gcatgtcgtc cagaggcatc
    13201 gcctcgagga cgacaggcag agccagcgcg acgcctccac gggtgagccg gagcgtgacg
    13261 cgctcccccg atcgaagcgc cgccatgccc gcatcggcgt ccagcccggt cgtaaacgtc
    13321 cgggcgccga tgccgaggag tcgatcgccg gcaaggagcc ccgcgcgcgc cgccgccgtt
    13381 ccgggtgcga cccgggcgac catgagcccc tcgccgggcg tgaagcggcc cgcggcgaac
    13441 ccgaggtcgg cgcgacgacg aagcggttcc gctccgcccg ccgggacggt cgcgacggcc
    13501 gccaggatga tcatcgcaag ggtcgagttc atcgagagcc tcccgtgccg gtcgctccag
    13561 gagagaaacg ccggaacgac aacgttggtt aacagcgacg gagcaagtgg ctcctggccc
    13621 gcaggtccgc ctcggtcccg cgcgccgcga gacgctcggc gactctcgcc cggtgttggt
    13681 gactccggtt gccgccgaac ttgaacttcg cgcgaacctc gtcgatctcc agtcgcaggc
    13741 cccggatgcc gccgagcatg cgtccgtacg gcgtgagccc gggctcgatc gaggcgtacc
    13801 ccccctcggg ctgcattcgg cgcatctgcc gccgaagaag cgccgcgacg ccggccggct
    13861 cgtccgggat gacggcggaa cagatcgcct ggaccgcggc gtagtagctc gtcggcggag
    13921 cccaatcggc cgccgacggg tcctcggcgt tccaggtgga gggaatgtag acgtgggcgt
    13981 cgatcaccgc gaagaccacg cgatcgttcg cccggatcgc cgtccagacc gggttctctc
    14041 ggtgcaagtg gagctcgatc gtccggtcgc cgtcgtagca gtagtgagcc gggacgacga
    14101 ccgggatctg gccgttcggg ccgggtgcga tgacctgccc gaagtcctgc tgattgagga
    14161 agtctctcca ctcctggtcg aggatcggtt cgtcggcggg tgtcgctagc acgaagggat
    14221 cctggcctct ttgctctcgc tcacgcgctc cgcccgctcg acgcggaaat cgtagcagcc
    14281 cgcctccgtg tcccgaacgt ggatgtagtc gacgtcattc cgcgcaagga gctgctcgag
    14341 ggccggcccg gcaccgcccc ccgtgacgta ctcctgcgcc cgaagcgagc ggcctcgtcg
    14401 ataggcattc agcgtgagcc cgtgcttccg gagcccgtcg gggaatcccc gtcctcgggg
    14461 tatcgaagac aatcggcctc gtgaatgaag acgggacccg gcaacggcaa ggtctcgagt
    14521 tctgcaaagg ggtcataggt gaagaggatc cgccgctcgg agccgatcgt gaacgtgcgc
    14581 aggcacaagc ggcacggccc gtgtccactc gcgacctcgg tgtgaatcgg gtgcccggcg
    14641 aacggcgatc tccggttcgc atgaacggac gcggcgacgg cctccggaat ggcgatgacc
    14701 cggtagttcg gcatgatctc ctccccacga caatctagag ttcgcgtgcc gcctggccac
    14761 ccgcttcttg cgcccaaagt cgtgaacgac tacacatctt cagccgagag cggacttgga
    14821 gcgttgccat caatgtccag gaagccgtac tcctgtgcca gcctcgccgc aaccagcacg
    14881 gtccccgatc tttcggtgat cttgggatcg gaagcgagcg ccacgaccgc gcggccgacg
    14941 aaccgcgggg actctgaatt gctcagatcc agccattgcg cggcctccat gaccctctcc
    15001 gtccgaacca ggccgggata cagcgagacc acggcaaccg cgtgatctga caattcccgg
    15061 gacatgtccg ccgtcatctt gtccgtcgcg gccttcgaaa cgccgtaggc gacgttccca
    15121 acgtatttgc tggccgccca gtacgagatg ttcacgatca ggccgctgcg ttgagagacc
    15181 atcacccgcg ccgccagctg agaggccaca tagtgagcgc gcaccccggc ctggaacatc
    15241 gagtcccatc gccacacggg ttgccgccag aaggggcgcg accacgtgaa ctcgccgttc
    15301 tcgagcatgt tctcgtagcc gccccacacg ttgttgacga gcaggtcgag acgtccctgc
    15361 tcgtcggcga ttcgacgaaa agcggcttcg acttgtgaat cgcggcggtg gtcgcactcg
    15421 atgccgatgc ccctctccga aacgtactgc atgtcgtcga cggagcgtcc ggtgacatag
    15481 acggtggcgc cggcgtccat cagctcgaga gcgataccct tcccgactcc acgactcgct
    15541 ccggtcacca gcgcgacctt gcccgtgagt ctgttcaccc cggctctcat ctggacggtt
    15601 ccccgccgct gttcccggtc cgtcacttct gaagccgtcc gaggtccttc tgcgcctgaa
    15661 ggtgtcccgg gacgagcttc agggcctcgc gcatctcggc cttggcgtcg tcaaggcgcc
    15721 ccagcttctc gtagagcagc ccgagccgcc agtgggcgtc ggcccaggtc gggcgcccgg
    15781 gcggcgcagg cacggcgagg tatgccctga gatgctcgac gcccttctcg agatcggtcc
    15841 cggagaagta cgcgatccgg gcgagttggt agcgggccat ggcgtccgcg gcgctagagt
    15901 cgaggcgccg ctgccagagg gtgcgggcgt cgccccagcg cttctgcgcc acgtagaagt
    15961 tgccgagcgc cacggccccg cgaaagccgg tgggatcggc ctcgagcgcc ttggagaact
    16021 gcgcttcggc gccgtcgtga tccttgtcgt ccgcgagaat ccagccgttg gcgatgtacc
    16081 cgcgcgcagc gtcgagcttc gcgagctcgg ctgcttgctc ccgggccttc tccttgctgc
    16141 ctcccacgat gccgggggcc tgcaggtagt agctgatgag gtcttcccgc gcgtcgacgc
    16201 tccttggatc gagcgcgacg gctttctcaa aagcggagcg gcacttcttc gcgaagccga
    16261 ccttggagaa gacggacgct gtctgcgcct tgcggccgta agcgcgcccg agccagagcc
    16321 acgtctcgga gtcgccccga tccgccgcga cggcttcttc gcccctcttg atcgcggcgt
    16381 cgaggtcgcc tttgtccatg tccgcccgaa tgccggcggc tgcccgggtc gcacccgcct
    16441 cctgagaagc cgcggatgcg gccccgcagg cgacgctcgc gacaaggacg gcgattcgga
    16501 gtgcggcttc tctcaatccc gcgacagcgt gcgtcccgga cgcaacgcgc gcgagcctgg
    16561 gtcccgtgaa ctgccgttct cggccgtcga atggcgcccg ttgtcacgtg tgtttcgcgg
    16621 cgggaacttc acggtgccgt cttcgtacct gaaagaaccg cttgccccgt gagtgcggcc
    16681 tgccctcgcc gcgaggagga atcatgacga tgaaggttgc cctgtttttc gcgacggtgc
    16741 ttctctcacc cacgaccgcg cctcgcaaca ccgcctcgac gagtacctcc acgatctccg
    16801 gagagcgctc gggggctccg cgaagagccc gtctccgaca tcgtgaacga gcggcggagc
    16861 cacgcggcgc tcaaggtgct acaccccgac acggccggtc tcgggattgg cccggaagga
    16921 tggggcgttc gccagttgcg gcccgcgcgt ccggccgggg aggcctgaca tgaaactctc
    16981 cgactcccgc gcgctgagcg ccctcgagct cctgttgggc accggggtcg tgatcgggca
    17041 caacgtgttc cggatcctgc ccaacgaagt gccgatcctc gtcgtgctgg gcctgatctc
    17101 gttccgggtg cgcagcggcg gctgggcggc gctgccgttt cgccgtccga aatcgtggcg
    17161 gcgcctcgtc tcgatcgcgg tcgcggcggc ggcgctcagg atcgtcctga gcggattcgt
    17221 ggaatcgctc gcctcgaagg tgtggcctcc gatcgtggcg cccgcgggaa gcgaagccat
    17281 agccggcgac ctcaagaccg cgctcatgtg gctcgggctc gtctggacgt gggccgcgtt
    17341 cggtgaggag ttcgggtatc gcggctatct gctcacgcgc gccgccgaca tcggaagagg
    17401 atccggggcc gcgtatctgg ccggaatggt gttcgtcgcc gtcctcttcg gcttcgggca
    17461 cttctacaag gggcccgccg gcatcgtaga ttcgggcttc gggggcctcg tgttcggagc
    17521 cgcgtacctg gtctcgggcc ggaacctgtg ggcgagcgtc ctcgcccacg gcttcgtgga
    17581 cacctacggg atcaccgcgc tgtacttcgg ctgggcgacg tgaatctcct ggctcgcccc
    17641 ggcgtctgtc gtgagcggtt tcttcatccg atgagccctt cggcgagccc tttgagcacg
    17701 gcgcgcgtgc ggtccctcac gcccagcttg aggagcacgc tcgagacgtg gttcttcacg
    17761 gttccctcgg agagccccat cgccgaggcg atctcgcgat tgctgtagcc gccggccatg
    17821 agccggagcg tctcgcgttc gcgcgcggtc agggcctccg gcgtctcgaa tccgtccgtg
    17881 tgcgccgggg atttttcgag gctcgccgct gcctggcggg tgaccgcggg ctggaagaac
    17941 gtctcgccgc gcgccagccg ccggatcgcg tccgtcagct ggtcgaaggt cacgtccttc
    18001 aggaggtagc cgcgcgcccc ggcgcgtacg gcttccagaa ggagcgcctc gtcgtcgaac
    18061 gtcgtcagga cgagcgtggg cgggagaaag cccgcggcgg cgaggccgcg gagaacgtcg
    18121 agaccgctcg gcccgggcag ccgcatgtcc agcaggacga cgtcgggctt caccgcaggg
    18181 atgagccgca gcgcctcgtc gccggtcgag gcttccccgg cgacctctac ctcgccccag
    18241 agctcgagga ggctgcgcag cccctggcgg acgagagtct ggtcctcgac gaggacgacc
    18301 cggatcatga gccgccgccc ggagcgcagg gaatgcggat tgccacctga aagcctctcc
    18361 ccggagaggt cgtgacggcg aaggtcccgc gcgcctcgga aatcctctct gcgagcccgc
    18421 tgaggccgcg gccgtccgcg atgcgggcgg cgccggcgcc gtcgtcctgc gcgagaacct
    18481 ccagcccgta cggcgtgcgg gtgacggcga tccagaggtt ctcggcgccg gagtgcttca
    18541 ccgcgttcgt gacgatctcc tgaaccgagc gcaggagcgt cgccgtgacg gccggatccg
    18601 cggcgagccc gtcccccgcg atgtcgaggt ggatcttcgg ccgcgggatg tcggacgcga
    18661 ggaggcgcag cgcgtccgag aggctcacgc ggtcgtcctc gcggaacccg ccgacgaccg
    18721 tccgcacgtc ggccagcagc agccgtgcga gcgaccgcgc ccgctcgacc ggctcgcgca
    18781 ccctgccgtc ggcgatctgt ccggcgacct cgagctgaag actcagggct gcgaggtgat
    18841 ggccgagcgc gtcgtgcaag tcccgcgaga tccgaagccg ctcggccgcg cggctgctct
    18901 cctgaagaag cttctgggcc gacacgagct ccgtgttgag ccgggcgagc tcgctgcgct
    18961 gtcgggcctc gcgctcggcg atcgacaccg tgtagaacgc gaacaactgg aaggcaagga
    19021 acgcggcgaa cacggcgccg ccgttcaggg ccccggactt cgtcacgagg aggaccgcaa
    19081 agacggcgtt ctgagtcccg caccacagca gcgcggcgcg cggcgaaagg cagaacggca
    19141 gctgagcggt cacgatcacg aggaggattc cggccgcggc cgagtcgacg aggaagacga
    19201 agagcagcgc ggccgcggtc tggacggcga gaagcgccgt tgcggccgcg cggtttcgcc
    19261 gcgctccggg gccgcccgtt gccgcgaaga aggcgaggcc gaaagcgaga aggaccgccg
    19321 agccgagggc gatgaccggg tccggtggcc cggcgcgcgg acgcagaagc aggcggatca
    19381 gcggaaggcc tgcggcgccc cacgcgagga ggccggcgat ccggagagtc gcgagagatg
    19441 tcggagcgtc tggcatgtcg ctcggcaccc agtgtagcgg cgcgaacggc cggcggcccg
    19501 tgccggaagt cacgccgacc ggcatgacga ccggcacagg gaaagccgcc tccgccgccc
    19561 cagattcacc tcatgaacag gcgtacgttt ctcgaactcc tgggcgccgg cggcgccgcg
    19621 gctctcctca cggctccggg cggcggttgc gcggccaagc cgctcggggc cctccgcccg
    19681 tgggagggac cgcccggtcc tcccggcgac ggcgacccga ggctccgcct cgcgtcgtgg
    19741 gcgctcctcg cgccgaacgc gcacaacagg cagccctggc ttgtcgacct tcggggcgac
    19801 ggcatggacc ttttcgtcga tccgcgccga ctcctgccgg aaacggaccc ggcgagccgg
    19861 cagatcatga tcagccaggg gacctttctc gagctgctcg cgatcgcggc cacacacgaa
    19921 ggccttctcg cccggatcga gttgttcccg gagggcatgg tcccggccgg agacgtgggg
    19981 cagcggccgg tcgcccgcgt cacgctcacc ccgcaacggg atcttcggaa ggacgagctc
    20041 ttcccgttca tccggcaacg ccacacgaat cgccgcccct acgcggaccc ggcgcccacc
    20101 gatcgggaag tcgcggacct ggccgcctcg gccgaaggcg cgggaatccg gttccgttgc
    20161 ctgcgtgagg ccccggcgct ctctcgtgcg gcggccctca tgacggaagc gatgcgaatc
    20221 gagacggcga cgaagcggat gcacgccgag acggtggcga tgctccgctt cgacgacgcg
    20281 gagacggaac agctccggga cggaatcacg ctgccgaacc tcggcctcac gggctttcgc
    20341 ctcctcgtcg cgcgttcctt cgtgagccgg gaaagagcat tcagccccga cttcctgaag
    20401 aagaccgtgg acgcgacgag agcgcaggcc ttcagcgcgc cggcgatggg acttctgtcg
    20461 acggccggcc gttcccgggc ggacgaggtg aacgtcggaa gggcgttcgc gcgcctccac
    20521 ctgacggcga cgaaactcgg gctctccctc caccccatga gccaggtgct cgagatcgaa
    20581 tccgagcgga cgcggttcgt caccgaagtc ctcgggtcgc cggcggaaga gccccagatg
    20641 ctgttccggc tgggccgctc cgccccgacg ccccattccc ctcggcgcga cgtcgcggcc
    20701 tgcctgcgga ccgaaggcaa ctttcgccgg acagattcgt aaggctggca tcgccggatc
    20761 ccgaaggagg ggagttgttt ctcgtctggt cggtctcagg cgtctccgcg atcctcgccg
    20821 tcgcgtgcgt cggtctcctt cgacgcgact tccggaccgc cccccggagt tggaggtggc
    20881 tcgcagtttc cggcctgccg ttggctctgg cgggcctcgg gttcattctc aggttctggc
    20941 ggcccctccc cgggccctac ctcgccaacg agtcgtaccc gctcgggccc tacgtcaata
    21001 cgtgggccgt ctcgttcggc ttcatgtggc tcgcctacgg tctggcgttc ttcgccctcg
    21061 cgatccggat gccgagggat gcgcgctcgt gggcggtcct cctcgtgacg tggatactcg
    21121 cgtggctgcc tcatttcctg atcggcgtcg gcttcgcggt ggccggctcc aaccgcccga
    21181 gcgtcggaat ctaccgggct tgggcgtcct cgtggccggg cttgttcgtg ctggccggca
    21241 gcgcgatcgc catgctcctc cactttgttt tcgcggcttg cgggttttct ctggccgcac
    21301 tccagatccg gcgggcggaa ggcgacggcc gctagacgac ccggcgccgc agccgcaccg
    21361 acgccgcgcg gctcgcgagg acggtcgtcc cgtccttgag cgtgatgagc aggcgccggt
    21421 cgtccgcggc ccggagatgc cggacggcgc ggaaattcac gatgtgggag cggtggacgc
    21481 gctcgaaaac ggccgggtcg aggcattcga ccagctcgga cagcgtcgag acgacgaggt
    21541 acgaggactt ctccgtgaaa acctcggagt actcgccgct cgcgctgatg cgcaggatgt
    21601 cgtcgacctc gatcgggacg atgcgcccgc cggtccgtgc gaaaaggcgg cgcagggggt
    21661 gccgcgtgac cgcgagcgcg cgctcgacgg ggcccgcgaa ggcggggtcg agcgcgcgcg
    21721 ccgccatccg gacgcgcgcg cgctcgatcg ccgcttcgag acgggcgggt ccgaagggtt
    21781 tgacgaggta gtcgagcgcg ccgagctcga acgccgagac ggcgtagtcg tcgaacgccg
    21841 tcgtgaacac aacctcgggg cgatgacgga tccggcgcag gacctcgaag ccgtccatct
    21901 ccgggagctg cacgtcgagg aggaggacgt cgggcttctc gtcgtccacg agccgcacgg
    21961 ccgcttcgcc gtcgctcgcg acgccgacga ccgtgacgcc cgagatatgt tcgaggtagt
    22021 cgacgacgtt ttgccgggcc tcgggctcgt cctcgacgac ggcgcagcgg agggtggcgc
    22081 tcattccccc tctccgtccc cggttccggc gccggcaggc tctccctcga gccgcagtcg
    22141 cgcgcgcgta ccgccgagag gggatgggcc gacgtcgaga gttccccggc cttcgtagtg
    22201 ggcgtcgagg cggtcgcgga ggagcttcag gccgaggccc gagcccgggc gcctcggcgg
    22261 cgcggcggcg ggggacggca ccccgtcgtc gtcgacggtg acgaggaggg cggccccctc
    22321 cgtgccgatt ccgatcgaga cgacgccgcc gcgggcgcgc gccgagatgg agtgcaggac
    22381 tgcgttctcg acgagcggct ggaggacgaa cggcggcacg gtacgatcga gggcgccttg
    22441 ctgggcgcgg acgcgcacgg agaggcgctc gccgaggcgc agccgctcga gcccgaggta
    22501 ctcgagcgtg aacgcgagct cgtcgcgcag cgtcacgctg tcgcagcgcc ggttctggac
    22561 gcggagcgcg taccggagcg tcgtcccgag gcgctcgagc gcggaggatg tggcgtccgg
    22621 gtcgcgccgg gcgaggccga cgagcgaatg aagaacgttc agaatgaagt gcggattgag
    22681 ctgggccctc aggatggaca ggcgcgcctc gagacgaagc atctcggcgc ggtgggcccg
    22741 ctcggcggcc tcggccgccc gccgcgcgtg gaagcgcgcc cgtccgacgc cggcgagcgc
    22801 gccgaagacg aggagcgaga acaggcactt ccatgcgatg ttccggggat ctgtgggaac
    22861 gggccacgag ccgtgcccga ccgttctcca gacgatgaat ccgcagaggg tcccggccac
    22921 gcaccaggcg atgaacgtgg ccccgaggag cgtgaggcgc agcccggtcg ggaaccgatg
    22981 gcgtccgtcg cgcgcggtct cccgcagtac gaacggcgct ccggcggctt ccggcccagt
    23041 gttcaccagg gcgacgagca aggcgtcgag cggactgaag ccccgcgcaa ggccgaacgt
    23101 gaccgcgaac acgaggaggt agagaaggta ggccgcgatc cagccgattc gcacggcggt
    23161 cgcgctcccg cctgaaaccg gatcttcgag cgcccggggc gccgccgctg catggacgtc
    23221 gcggatctcc acgacgcgag tctctcgcgt cgaaggctct gcctcaaggc aggatggcgc
    23281 cgttcgagga acgaaagcgc ccgttcagaa ggttccggct cgcgcgggcc gcgtctgtgc
    23341 cgcagtttcg ggaagccgcg gcgtacgtcg cggttcacga ggaggctgac cggtcatgaa
    23401 gctcgttccc gcgctcgctt tcgccgccgc cctgggctcc gccttcgtgg cgctgtcggc
    23461 ccccgtcgcc tcgggagcct cgagaacgaa gggttcgaat ttgtggcggt gctattcggc
    23521 ctcgggcact acgacagggg ccccgtggga atcgtggact cggcctttgc cgggctcgtg
    23581 ctcggcgccg cgtacttgat ctccggccgg aatctctggg cgagcgttct cgcccacggt
    23641 ttcatcgaca cgttcggagt cttcgcgctc tatctcggct ggtcgagctg aagtccagcc
    23701 attcatcatc catgaggagc ctagaatgat tctccgccgc ctcgtccttc ctcttctttt
    23761 cgcctcaacg gctccgggcc agatccccga ttccttcagc aacctgcggt tcttcccgaa
    23821 ggacatcccg aaagcgcgcc tcgtcgagga gatgaagtcc ttcacgctgg cgaccggtct
    23881 gcgctgcgac ggatgtcacg tgggcggacc cgggctcgac acgatcgact tcgcgtcgga
    23941 cgagaaggaa accaagcgga ccgcgcgcgc gatgctcgcg atggtgcggg cgatcaacga
    24001 cgaccatgtc ggacgcctcg gccgcgcaaa ggacgtgaag gtcgtctgcg ctacctgcca
    24061 tcacggcgtg tcgaaaccgg ctctcctcga gaagttgctc gccgcgaccg tgcgggaaaa
    24121 gggcctcgac gctgccgtcg cgacgtaccg cgagctgcgg cggacgtact acggcagggc
    24181 cgcgtacgat ttcggcgaag cgacgctgaa cacagccgcc ctcagcctca tcgccgaggg
    24241 caaggcgaaa gaggcagtca ggctcgtcga gctgaacctg gagttcgggg cccgcgaggc
    24301 gctcacctac aacgtcctga gcgatgcgcg actcaccgcc ggggatcgtc cgggcgccat
    24361 cgcggcgctg aagacagccg tcgagatgga cccgaaggcg gacttcctcc gaaagaagct
    24421 ggagagactc gagaaagcgg cgggccgggt ggacgggtga cgagacgcac gcgctggatt
    24481 ccctgggcgg ccgttctcgt tctggcttcc gtggccggcg tgtggcgcgg cgggcagtgc
    24541 aggccccccg actttccccg aagccccgcg ctccacgcgc gtctggaacg gacactcacg
    24601 ggccatgcgg ccgtggtcgg gcaggtcgcc ttcagcgcgg acgggcagtt ccttgccagc
    24661 ggtagcgcgg accgaaccgt gagactctgg cgttggcgcg agggcgtgct cgctcggacg
    24721 ctcacgaatc ccgagggggt cacctccgtc gccttcagcc cggacgggaa ctggctggct
    24781 tgcggaagct acgaccggac ggtcaatctc tggcggctgc gcgatggcat gctcgcgcag
    24841 accctgcggg ggcacgaagg cacggtgtgg tcggtcgcct tcagtcccga cgggcagcgg
    24901 ctcgccagca gcggagaaga caggaccgtg aggctctggc gcgtcagcga cggcgcgccc
    24961 ctgcgaaccc tttccgggca tggcctgaac gtctggtccg tcgcgttcag ccccgacgga
    25021 cgctttctgg ccagcgcgag cttcgaccgg acggccaggg tctggcgctc cgacgacgga
    25081 tccctcgtgc gcacgctcgt cggccactcg caggcaatcg tctcgctcgt cttcagtctc
    25141 gactccgagt tgcttgcgac cggcggcgac gattcgtccg tcaggctctg gcgcgcgaac
    25201 gacggtgctc tcgtgtcgac cctgacgggg tgttcggagc acgtctacgc ggtcgccttc
    25261 agccccgatg ggcgattcct ggccagcgga ggccgtgaaa gaggaaacct ggggacgctc
    25321 tggaaaaagg tcatgggcga gcgcctgagc ggcggcaagg gtccgacgat ccggctctgg
    25381 caggttcgcg atggcgtcct gcagcaggcc ctggccgagc attctgacga cgttcgctcg
    25441 atcgcgttca gtccggacgg gcaatggctg gccagcgggg gcgaggacaa gacggtcatc
    25501 ctgtggaggc tcgaggcgca accgtgatcc cgccgacttc cgcgacgagc ggccaagcgg
    25561 ctcagcgcga tccgaccatt attcgagaga gcgaggcgtt cggggcagac ctcgagtcgc
    25621 gggcgagggt cacgcgcgct ccgccgtcga gccggccgca gcccgtaccg tcacacttca
    25681 aggttgcgac ttcgccgttc gtgggtcggt tccggcgttt cgccggcgac gggctcgagg
    25741 acgatcgcgg cggtgcgaac ttcgcgttcc ccggaacgta ggaggacctg caacaggagg
    25801 tttgacgcca tgagacacgc cctctcgttc ctcgcggtcg cactcgccgc ggccccgctg
    25861 gccgccgccc ctcccaggac ggcggcctcc tctaaccgcg ctctcgccac ggcggcggcc
    25921 gacattctgc gaaacgacta ccgggccgac cgcgcggagc tcttccgtct cgctcaggcg
    25981 ctcgagagca ccgccgcggc cggcccgaac gagaagtacc ggcggtactg gattgctttc
    26041 gcatactggc gccgaggatt caacgggctc aacgagacgc cgaagcccgc ggatctcggc
    26101 gaggacttcg tccgctgcga ggcgcacgcc cgcgccgccc tcgccctcga ccccgccttc
    26161 gaggacgcgc gcggcgccct catggggtgc ctcatgggcc ggctctgggc gggcgccgtc
    26221 cccgacgacc ggaaggccga gtggatccgc cagggcacgg aggtcatgca atccctgatg
    26281 aagaacgcgg gctcgaaccc gcgcagcctc tggctcgtcg gaatgcagca ggcctaccgg
    26341 cccgcgggcg gggacacgaa cgtcgccgtt gcgacgtgga agaagggtct cgccggggcc
    26401 cgcgaggagg cccttgcggc ggcgtcgcgc gacccgtggg ttccgtcttg gggcgctccc
    26461 gagatcctca tgaacctcgc gaacttccac acctacacga agccggacaa ggccgtggcg
    26521 cgggcctacg cggagggcgc actcgcgatg tctcccgagt ggcactacgt gcgtgacatc
    26581 ctcatgccgg ccatcgagaa gctgccggac ccgccggccg ctgcggccgc gactccgccg
    26641 ccaggccggc cgagcgccgc cacgcccgcg ccgacgggtg gtcgcccgtg aacctccggg
    26701 agatcctcgc gcccgaatgg cgcatcgagg ccgcgacgac gcgccgcgtg ctcgaacgcc
    26761 tcccggtcga tcgtctcgac ttccgcccgc acgccgcctc ccgaacgctc ggcgagctgg
    26821 ccgcccatgt tgccgtcatt cccgagctct tcctcgcccg gctgcccgag gaaggcctca
    26881 gcatcgacgg cgacgccgcg cctccctcag accctgcggg cctcctcgag cgtttcgacc
    26941 ggagctccgt tcgggccctc gaggcgctcg agagcctctc ggacgagcgc ttcctcgagc
    27001 cgtggaccta ccggcgaggg gatcgggtgg tcttcacgct gccgcgactc atcgtcgcga
    27061 ggacaaccgg gctcaaccac atcgttcacc accgggggca gctcacggtg tacctgcgta
    27121 tcctcggcgt ccccgtgccc tcggtctacg gcccgtcggc ggacgagcgc gtcgtcggcc
    27181 cacgttctgt aagcgaaaga gaaccaaagt gacccttttc gacttgatct tcatcggcgt
    27241 cttcttggtc accgtcacca tccttctggc gactgcagtt gcggccgttc gagggaaccg
    27301 cccgcgtgcc cttggcctcc tgcgaggact ggggggcctc atcggcgtct atctcgcggt
    27361 cgtcatcgtc gtgtccctcg tttcgccgcg ccggttgatc cccatgaaag aacctcggtg
    27421 cttcgacgac tggtgtgtct cggtcgatcg cgtggatcga acgccggcgg gaacggaggt
    27481 ctcgtacgcc gtgacgcttc gcctctccag ccgcgcgcgg gcccgcgcac aacgcgagaa
    27541 cggcgtcagc gtctatgtgc tcgacgatcg aggccggcgc ttcgaaccac gggaagaccc
    27601 gcaggacgtc cccttcaacg tgttgctgca tcccggcgag tcggtcgacg ccacgaggcg
    27661 gttcgtgctt ccggcagatg tgcagggtcc ggggctggtg atcgcccacg agagattccc
    27721 gggcatcttc atcatcgggg acgaccagag catgttccac aagccttcgg tgacccggtt
    27781 cccgtagtcg gcgatctcct cgacgtccgc tgcgccgtcg gccgtaaccg gttgtcaggc
    27841 accctcaatt gtgcttgggc tcgtgcggcc tgaggcccag aaagacctcg gacccaggcc
    27901 cgacgtcgct ttgctcgaac gggacggctc ttggctcgaa cccggcggcg ccgatgatcc
    27961 gcagccagtc ggcgcggcgg aacaccccga gctgcagccg atccgactcg acgcggagcg
    28021 cgccgtcctc gtcgcggagc aagtaggcga agtccacgag gtaggtcgag tcgtcaggat
    28081 ctggatccca cgtccattcg agatatcgca tggctctacc gtttccgtcg tggccgccgt
    28141 ggctcgtcga gctgcggaag ttctcccgga catagtcggg agcaaagagt gcgacacctc
    28201 ccggcctgca gtggacgtat gcagagtgga tcgcttgctc cagctcggcc tccgttcgca
    28261 tgtacgagat tgcatcctgg acgaagacca cgtcgaagag acgcccgagg cgaatggaac
    28321 gcatgtcgcc cgaaagatgc tcgcactccg ggttgatcga ttggctctgg ccgagcatct
    28381 cgggtgagag gtcgacgagt gtgagcgtgc agtgactctt caagaacgag gcggtgttgc
    28441 cgccgccgct gcccaactcc agaagtgatc ggggcggtga cgagcaagca gaccggatca
    28501 cgttccagta gaaagcagct gcttcggcgt attctgcggg tgccgccagc aggtgccacc
    28561 acggagccag gtcggtgtag agcctgcctg ggagtgcctc agagccggga ccgtcagagg
    28621 gccgaaaagt cttcgtcatc tgccggaacc tacacgaaat gcccggaggg cggcgccgtt
    28681 cgacgggtcc gttcgcccgt tcgccgactc tgcccacccc gaggccctcg accgcctgaa
    28741 ggcctgacgt ctagaatcac ccatgcagaa gacattcgtt ctcgccgcgt tcgccgcgtg
    28801 gctcgccctg cccgccagcg cccagcagga cttctcgaag gtcgagatca aggccgagaa
    28861 ggtcgcaggc ggcctcttca tgctcaccgg cgccgggggc aacatcggcg tctcggccgg
    28921 gccggacggc gtcttcctcg tcgacgacca gtacgcgccg ctcaccggga agatcaaggc
    28981 cgccgtcgcg gcgatctccg acaagccgat ccgtttcatc ctgaacacgc actaccacgg
    29041 cgaccacgtg ggcgggaacg agaacctcgg gaaggacggc gccgtgatcg tcgcgcacga
    29101 gaacgtccgc aaacgcatgt cgacggacca gttcctcagg ctcttcaacc gcacgatccc
    29161 ggcctcccct gcaggcgcgc ttccgctcgt gacgttcgcc gagtccgtct cgttccacgt
    29221 caacggcgag gatgtcgacg cagtccacct cgcgtccgcg cacacggacg gagacgtcgt
    29281 cgtgtacttc cgcaaggcaa acgtgatcca tgcgggcgac aacctcttca tcgggaggta
    29341 cccgatctgc gacatctcct cgggcggatc cgtcgaggga atgatcggcg cggccgaccg
    29401 tctcctcgcc gccggcgacg agaggacgaa gtacatcccg gggcacgggc ctctcgggac
    29461 gaaggcggac gtcaaggcgt atcgcgacat gctggcggcc tcgcgcgacg cgatcctccc
    29521 gctcgtgaag gcggggaaga cgctcgacga ggtcaaggcg gccaggccca cggccgccct
    29581 cgacgagaag tggggaaagc cggacgtctg gacgatggtc gtctactact cgtacggcgg
    29641 caaggagccg gcgacgccgg cgcccgcgaa gtagcgtccc ggcgcctcgc ggtcagcgcg
    29701 cctcgctcga gccgggttcg taccgccagg cggtctgctc cttggcgccc tcggcgaagc
    29761 gggtgtcgac gtactcgtcg aactcgatcc ggcggtcgag gacgcccgtc tcgatcatga
    29821 ggtcgcggac gaggtcgaag tcggccttgc gcggcgagag cggcgtgtac atgacgcggt
    29881 cgagcgggtt cgtgagcgcc cagcgcagga ccttcgggtc ctggttgtag tagaagcgcc
    29941 cgacgaagtc cgcggcgtgg tcgcggttgg gcttgccgtc ctcgagccag aggccggagc
    30001 gcgcgatccc gtcgacgagc gtctggacgg tgcgcgggcg cgccgcgatg acgtcctcgc
    30061 ggacgacgag gacgcaggac atgtagtcgg gccagtgctc gcgggcgtgg aagagcaccc
    30121 gcccgtagcc gccgatctcg gcctgcgagg gaaacggctc gcccatcgag aacgcgtcga
    30181 tcgcgcccgc cgccagcgcg cccgtcacgt cgggcggcgc catctcgacg atcttgaggt
    30241 cctcgggctt catgccgaac ttcttcagga cgcggaagac gatgagccgc tcgtccgaga
    30301 aacggctcgg caccgcgatc gtccggccct tgaggtcggg gaacgtcttc accggcccgt
    30361 ccttgcgcac gacgaccgcg cttccgtagc ggtgcccgag gtagacgatg cggatgccga
    30421 ccccctgcgc cttcaacgcg atcgccatcg gggcgacgat gaaggcggcc tgcatcttgt
    30481 tcgcgatgag ggcctccttg atctcgggga agccctggaa catgcgggga atgaagagct
    30541 ctccactctc ggagaactgc gaaatgtagt ccgtgaccgg gcaggcgagg tggcaagtga
    30601 ccgggatgta cgccacgttc agcttgcgct tctcgggcgc gagaaagtcg ttcgcgagga
    30661 cggcccagtt cacgttgacg gcggcgtgga cgagggacag ggcgacgatc cacgccgcga
    30721 cgccgaggcc gatgcgcttc gcggcggtca cgcggcctcc ccttggaagc cccagcgcac
    30781 cgtcctgagg cgctcgagcc gccgcgcgag cgtgtcgagg aggaggccga tgacgccgat
    30841 gaggagcatg cctgcgacga cgaggtcgta gcgcttgccg gcgttgcgcg agtcgatgat
    30901 gaggtagccg aggccggagt cgacggcgat catctcggcg gcgacgacga cgatccacgc
    30961 gatgccgagc gcgatcctca ggccgacgag gatctgcggc agcgccgccg ggaagacgac
    31021 gcgggcgagg agccgcggcg tcgagaggcc gaagttccgt ccggcctgga ggtacatggc
    31081 cgggacgctg cggacgccgt tcatcgccgc gacgacgatc gggaagaacg cgccgagaaa
    31141 gatgaggaag cccggcgccg cctccgacac cccgaaccac aggatcgcga gcggcatcca
    31201 cgcgagcggg ctgatcggcc gcgccatctg gatgaccggg ttgaacgccg aggcgaggtt
    31261 cgggtgccag ccgagcgcga ggcccgccgg aatcccgagg acgagcgcga ggaagtagcc
    31321 ggaggcgacg cgccggagcg agtcgccgac gtaacgccac aggacgcccc ggcccgcgag
    31381 ctcctgaagg cccgagagca ccttcagcgg ggacgggaag acgttcgtgt ggctcagccg
    31441 gacggcgagc gcccacaaca ggacggccag cgcggccgtg ccgaccggca ggaggacgcg
    31501 ctcgaggtca gacctggtgg gcaaggccga tctccccgag gaggaggtcc ctcagctcga
    31561 tgtagcgcgc cgagctgagg tcgcgcgggt gcggcatctc gatgtcgacg atgcggcgga
    31621 tcgtggcggg gcgcgtcgac atgacgacga cccggtccgc gagctggacc gactcgtcga
    31681 tgtcgtgcgt cacgaagagg atcgtcttct tttcggcccg gcggatgcgc aggagctcgc
    31741 tgcgcatcac gaggcgcgtg atcgagtcca gcgcgccgaa cggctcgtcg aggaacagga
    31801 cgtcggggtt cacggcgagc gcgcgcgcga cctcgagccg ctgcttcatg ccgccggaga
    31861 gctcctgcgg gtacgcgttc tcgaacccct cgagtcccac gagtttcacg tagtgggcga
    31921 cgcgttgggc gcgttctttc gcgccgagcg cgaaaaggcc gaacccgacg ttgccgctca
    31981 cggtcagcca cgggaagacg ccccgctcct ggaacacgaa gatgcggcgc gggtccgggc
    32041 ccgagacgtc ctccccgtcg atgagaacgg agcccgccga tgccttcagg aagccgccga
    32101 cgatgttcag aagcgtggat ttcccgcagc ccgaggggcc gaggatgcag acgaactccc
    32161 cgtccgcgac gtcgagagat accgacgaga ggacgtcgat cggggcgccg tccgtcgcga
    32221 agctcctcga gacgttccgg aggctcacct tgggagacgc cgtcacgacc cgtaccccca
    32281 tctcagggcc ttcagggtct cgagccgccg gatcgccacg tcgagggcga ggccgatcag
    32341 gccgatcatg accatgcccg cgacgacgag gtcgtagcgg ttgcccgcgt tgcgcgcgtc
    32401 cacgatgagg aagccgagcc ccgagttgac ggcgatcatc tcggccgcca ccaccacgag
    32461 ccacgcgact ccgagggaga tcctcagcgc gacgatgagc tggggcagcg cagccggcag
    32521 gaccacgtcg aggaggaggc gccggtcgga caaaccgaag ttgcgtcccg cgcggagaag
    32581 cgcgggcggc acgttgcgca cggcgttcat cgccgccacg acgagcgtga gggcggatgc
    32641 gatgtagatg aggaagatcg ccgcgacgtc cccgactccg aaccagagga tcgagatcgg
    32701 aatccacgcg agcggcgaga tcggccggag gagctggagg atcgggttca gcgcctgctc
    32761 gccgcggcgg taccagccga ggaggaggcc gagcgggatc gccgtcacga cggcgagcga
    32821 gtagccccac gtcacgcgga agagcgacgc gacgacgtac ttcgcgagga ggcccttctg
    32881 gagcagctcg ccgagccccg agagcacctg gagcggatcg gggaagaggc ccgacgtctg
    32941 gaaccgcacg gcgacggccc agaggccgag cagcgccgcg gccgcggtga acgggaggag
    33001 tcgcttcacg gcctagttgc gggccggcgc ggactcgggg acgccgggcg cagggacggc
    33061 acgggtcgcg cgcggcggga ggatcggaac cggtccgccc acggtccggg gcttgacgac
    33121 gtacgtccag gcgttggcga gctcggcccg ggcccagcgc aggaccccgt gggcgagcat
    33181 gacccacgcg gcgctccgga ccgaaaagcc gacgaagccc ggacgcttcc cggcgttgtg
    33241 gttgccgtag atgccgagcg ccttcttcct cttcgcgatc gcgacgaggc ggcggttgta
    33301 gaagcgcatc gggtagaacg cgatctcgga gccgtcctgg tagtacggcg ggaggccgag
    33361 gcgttcggag gaccacctgt aggtcgggat gacggcggcg aggtagtaga gggccgtgtc
    33421 catcaggaac gtcgtcgtca tggtgtcgta gtcgcccatg acgtggtact tgtcgcggaa
    33481 gatcgcctcg tagaagtacc ggaagaaccg cgcgtagcgc gtgttgtgga gctcgtactc
    33541 cttcgccatc tcctcggggg gggcgcccgc gagggatttc tggatcaggc cgacgcggga
    33601 gtagacggaa aacgccatct ggtcgaggcc cggcgagtag aacgggtcga ggaagccgcc
    33661 ggcgtccccc acgcacgtcc agcccggccc gacgaacgtg tcgacgaagt aggggaggtg
    33721 gccgtacatg cggcagtcgt ccgcgacggg ttcggccttc gcgaggagct cccgcgtcag
    33781 cgggttcgcg ttcaggaagt tcgtgagctt ctcgagcggc gtcgcgccct ccggctggac
    33841 gaggcgcgtg tcccacacga ggccgacgct cgtctcgccg ccctggagcg ggatgaacca
    33901 gatccagtag ccccagcccg tgaagtggtt cgtggcgagg cggcgggccg tcaggaccgc
    33961 ccggtgaaac gggtccgagg cgtccccggt gaggaactcc gggccgtcca tgtccaggac
    34021 gccgcggtag cggacccaga tggccgaggt cgggtgggac tcgatcggcg tcagcccgcc
    34081 gcgcttccgc gcgagcatcg cctggcggcc cgtcgcgtcc acgacccact tgcaggagac
    34141 ggtctgccgc gagccgtcgg ccttctcgac gacgagcgtg ttgccggcct cgccgttcag
    34201 ggccacgtcg acgaccttcg cggggcgcca gaccgtcgcg ccctccttcg ccgcgagggc
    34261 gaggacgtgc tcgtcgagct tctggcggtc gatctggaag gacggcgtgc gcgcgagctg
    34321 cgtcggcccg gtctcggagg cctcacgcag gcacgagacg ttctcgttgt ggaaccagta
    34381 gcggaacgcc tgcttcggga gctgctcgcg cgagaggtgg tcgtagagct tcagctctcg
    34441 cgtcaggaag taggccgaga tctcgacggt ggactcgccg accttccagt cgaaggcctc
    34501 gctcttctcg acgacgagga ccttcacgtc cggcatccgg cgccggagga ggaccgccgt
    34561 cgaggcgccc gagatcgcgc cgccgacgac gacgacgtcg aacgcgaggc tttcctgaga
    34621 cgggatctct tgcacggccg gaagttaccg gattccgtgc ccctcgtcca cgacgagggt
    34681 ccggtcgacg gtcgcgacgc ttccgaggtc ctggacgaag ccggacggcc agcgcaggac
    34741 gagcctttcg accctgctcg cgcggccgag gccgaggtgg acgatcgggc tcccggtgga
    34801 gaggtacccg ctggcatagc cgctttccct cacgaggaca cggccgtcgg cgagcgtcgc
    34861 gctgacgcgg acgcccagcc cgtcggcgtt gtccttcccg cgtgtgccct tgagtcgaag
    34921 acggaggaaa tgctcggcat ccggctccct gcggctgccg aggaagagag cctccggcgc
    34981 ctgcacggac ctcacgtaga cgtcgagcgc tccgtcgctg ttcgcgtcga acgcgacggc
    35041 ggcgcggccg ttcgtctcga ggtcgaggcc gtcgaggaac ccgcgctcct cgaaagcagt
    35101 cggccccggc gctcggttga cgaagtaggc gtcccgctcg acgccctgga cgccggcgcc
    35161 ctgccggtcg aacgtcttcg tcttcgcgat gtagtcgtcc cagtacgcga gcgtctccca
    35221 ccagaactcc agctctcggt cgtggacgcg gccgtctccc cacatcccgt tcgtcgcgta
    35281 gacgtcgagt cgcccgtcgt cgtcgaggtc cgccgcgatc gcgctccagt tccagcccgc
    35341 gcggccggcg ccggagcgcg ccgtcgcgtc ctcgaagcgg cccttcgccg tctggaggag
    35401 gagcgagttg ccgcggcaca tcttctccat ccaggagatc gcgatcggcc ggaagatccg
    35461 gcccgggagc ggcacgggca ggccgggata ctcgtggagg aatccccact gggtgtacgt
    35521 gcccgtcgtg tagaggtcca tccggccgtc gccgtcgaag tcgccccacg cggcggacat
    35581 cccgtagccg cggtcctcgg cgccgagctt cgccgtcacg tcctcgaagc gcctgccgtc
    35641 cacgttgcgg tagaggttct tcaggccgaa gtcgttcgtc acgacgaggt cggggcggcc
    35701 gtcaccgtcg aaatcggcga agaggcacga gagcgaccag cgcgtgggct ttgcgacgcc
    35761 ccacgcctcc gacgcgtcac ggaaatggcc tttcccgtcg cccaggaaca gatagttccg
    35821 ccgcccgtcg ttcgcgtcga acggcggatc gggcatctgc ccgtagtagt cgcccgtgcc
    35881 ggagacgaag aggtcgagac tgccgtcgcc gttcacgtcc gcgaacgcga gggagcgcgt
    35941 gggacccgcc gtgacgactc ccgaagaggc cgtgatctcc tcgaacgtcc cgtcgccgcg
    36001 gttccggaag aggcgcgtgg gcccgaacgc gtccgtgacc gcaaggtcgg ggaagccgtc
    36061 gccgtctacg tcacccgccg cgacgccggt cgcggggacc ccggagccgt cggccttcgc
    36121 gaggccggcg cggaccgtga cgtccgtgaa ccgcccgtgt ccgtcgttgc ggtagaggat
    36181 cgagcggaca ccgtctccga cgaagaggtc ctcggcgccg tcgccgtcga agtcgaggac
    36241 ggcgacgccg gacccgggcc agatgccgtc gatgaggcgg ttcgtgagcc tctgcggcgg
    36301 gtcgcggcgc ggcgcgccga ggcccgccgc gagcgtcgcg tcgtgaaagc ggggccgcgt
    36361 ctccacgcgg tcgccgccga gaggggagaa agaaagagag aagaaagacg aagaagggat
    36421 ttctttgaag gtaagagggg aggggagcgg cgcgttcgcg tcagggagca ggagcgtcac
    36481 tctcgccgcg cgctccacgc gagcgccgtc cgcgcgaaaa ccatccacga cgaaggtcgc
    36541 cgtcccccga agctcccacc gttcctcacc ttctaagaaa atcttctctc cggtcttctt
    36601 tctctcgatt tccggcttcc catcgacgtc gaacgagacc atcgcgacgc ggtcgaggtc
    36661 cttccacgcc caggcgcgga gcgccgcgat gccttccgcg tccccgggat gcgcgctctg
    36721 cgcgcgcagc gccgcgcgcg tcgtctcgag cgcgtcgcgg tagcgctcca gacggtgcat
    36781 ccacgggacg aacgtggccc tcagccgcgg gagggaggcg ttctcgtaga accaccagcc
    36841 gagcgcggcg agtgccgcaa ggagcgcgat cctccggcgg ctcatcgcgg cctacgtgct
    36901 cgccttcgcg cgccccggct gcacggccgc gacgaacgtg agcttcgggt tctcgcgctg
    36961 cgcgcgctcg aggtgccacg ggctctggaa gaggacgagg gggcggccct cgaggtcgac
    37021 gagcgacgcg acgctcggcg ggtcgtcgaa acgcttcggg tcgaatccct cgccctcgac
    37081 ccagcgcgcg agcgtgaacg gcaggcgcgc gtacgagatc tcggccccgt actcggcgcg
    37141 cagccggtgc tggatgacct cgaactgcag gacgccgacg gcgccgagaa cggggtcgcg
    37201 ctccatgcgg tccggatcgt ggaacacctg gacggcgccc tcctcggaga gctggtcgag
    37261 ccctttcttg agctgcttgc gcttggcggg gtcgagcatg acgacgcggt tgaagtgctc
    37321 gggcgtgaac cgcgggatgc cctcgtactc gatcgccgcg ccttcggcga gggagtcgcc
    37381 gatccggagg ttgcccgggt cccagaggcc gaggatgtcg ccggcccacg cctcctcgac
    37441 gagcgtccgc tcctgcgcga ggaacgagag gggcttggcg agcttgagga tcttgcccgt
    37501 gcgcgcgtgc gtcgcctcca tcccgcgcgt gaacctcccc gagacgacgc gcacgaacgc
    37561 gacgcggtcg cggtgcgcgg ggtccatgtt cgcctggatc ttgaacacga agcccgagaa
    37621 gcgggggagg agggggtcga cgaggccgcc ggaagaagcg cgcggcagcg gtggaggagc
    37681 caggtcgagg aacctctgga gaaagggctc gacgccgaag tcgttcactg ccgaaccgaa
    37741 gaagacgggc gtaatgccgc ccgcgaggaa ggtcgcgcgt tcgaacgcgt gcccggcttc
    37801 gaggagctcg agctcgccgc gcagcgtcgc gtgggccgtc tcgccgaggg acacgacgaa
    37861 acccggatcg tccagcgacg tcacgccgac cggcgccact ctctcccctt tcgcagaaaa
    37921 atcccctctc ccgtatctca ggagcgtctt cccccagcgg tcgtacacgc cctggaagtc
    37981 cgggcccgag ccgatcggcc acgtgacggg gtggcagggg atcccgagca cgcgctcgat
    38041 ctcgtccatg aggtcgagcg ggtcctttcc ggctcggtcc atcttgttga cgaacgtgac
    38101 gatcgggatc cggcgcatcc ggcagacctc gaagagcttg atcgtctgcg gctcgacgcc
    38161 tttcgcgctg tcgatgagca tgacggccgc gtcggccgcc gccagcgtgc ggtacgtgtc
    38221 ctcggagaag tcgttgtggc ccggcgtgtc gaggaggttc acgtggtgcc cctggtaggg
    38281 gaactgcagg acgctcgtcg tgatggagat cccgcgctgc cgctcgagct ccatccagtc
    38341 gctcgtcgtg tggcgctggt tcttgcgcgt cttcacggag cccgcgaggt ggatggcgcc
    38401 gccgtagagg agcagcttct ccgtgagcgt cgtcttgccc gcgtccgggt gcgagatgat
    38461 cgcgaaggtg cgccggcgcg cgacctccgc ggcgagggtc cggacgtcgt cggtcacctc
    38521 agtgattcac cgacggcgcc gggatggcgc ccggggtctc cgagagggcg aacgcgtact
    38581 cgtcggcccg ctcctcgacg gcggccttca cgaggtcggc gccgccgtgc cccgcgtgct
    38641 tctcgatccg gaggaggacg gggccgcccg tcgaggccgc ctgcagggcg gccgcgaact
    38701 ttcgcgcgtg catcgggtcc acgcggtcgt cgctgtccgc ggacaggagc aggacggagg
    38761 ggtacttcgt cccggccctg aggtggtggt acggcgagta ggcgtggagc gccttgaact
    38821 gcgccgcgtc gtcggccgag ccgtactcgg agatccacgt cttccccgag ccgaagaggt
    38881 ggtaccgcac catgtcgagg agcggcactc cgcagagcgc gacgcggaag agcccgggat
    38941 gctgcgcgac catcgcgccg aggaggagtc cgccgttcga gccgccgcgc aggacgaggc
    39001 gctccgggga cgtccacttc tccctcgcga gatacgccgc cgcggccgcg aagtcgtcga
    39061 agacgttttg cttcttcagg agcattccgg cctcgtgcca ctcctcgccg tactcgccgc
    39121 cgccccggag gttcgggacg gcgtagatcc cgcccctctc gagccacggg aaaagcgttc
    39181 cgctgaaatt gggcgtgaag gacacgagga agccgccgta gccctcgagc atcgcgcgtg
    39241 ccgtcccgtc gcgatggaag tccttgcgat ggacgatgaa catcgagacg cgcgtgccgt
    39301 ccttcgacgg atagaaaacc tgcgtgaccg tgtagggcga cacgtccgcc ggcaccttca
    39361 cctggaagta cagcgacgtc gcgcccgact tgaccgacgt cttgtagatc gacgtcggcg
    39421 tcgtgaagga cgtgaacgag aagtacgcct cgtcctcgtc gtcccgtccc gaaaggttgc
    39481 tcgcggaccc gatgccgggc agcgggactt cgcgcagaag cgttccgtcg agatttcgga
    39541 tctcgaggag gctcgacgcg ctcttcaggt agtcgagggc gagctttccg ccgacgacgt
    39601 ggaagctctg gagtgccgcg tccctgcgct cggggacgat ttccttccac gccgcgcggg
    39661 cgggcttcgc ggggtcca
//
LOCUS       DAOMFY010000006        38946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_328_length_38946_cov_9.660178, whole genome shotgun sequence.
ACCESSION   DAOMFY010000006 DAOMFY010000000
VERSION     DAOMFY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 38946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38946
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_38946_cov_9.660178"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1073
                     /locus_tag="PLB01_01235"
     CDS             105..1073
                     /locus_tag="PLB01_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HQQ75952.1"
                     /translation="MKTKNSFECECARARGVLFGLLLALPLLLAFEGNLSAAAAAPKQ
                     KSFSSPKAAAEALISAAARFDVPALTEILGPDGADLVVSKDPVQDRNTAAAFARKALE
                     KHSVVTDPGNAGRATLVVGDEGWPSPIPIVRKGGRWLFDTKAGRQEILYRRIGRNELD
                     AIEVCRDFVRAQHAYALQKHDGSRVNQYAQKIISTPGKQDGLAWRGPDGAPAGPLGDT
                     IAEAIAAGYSKKSEPLHGYTFKVLKGQGPAAPLGEMDFVIEGAMIGGFALVAAPADYR
                     VTGVKTFIVSHTGVVYEKDLGPKTLEQFQAMERYNPDKTWRPVKEP"
     gene            complement(1122..3548)
                     /locus_tag="PLB01_01240"
     CDS             complement(1122..3548)
                     /locus_tag="PLB01_01240"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQQ75953.1"
                     /translation="MRKLSTRAGFCIALSLVCAPRLGAQPVPDRTVLPIHEPQYPHST
                     VLDVRNAPPPPPRFEVKAPAGAPNVLIVLIDDMGFGQSGAFGGPLNMPTVDRLAQSGL
                     KYNQFHTTALCSPSRAALLSGRNHHMTNTGSIMETSTAYPGNTGQRPEAVAPLAAMLR
                     YNGYTTAHFGKNHETAAWEVSPSGPTDRWPTRNGFDKFYGFMGGETNQWAPAVYDGMV
                     KVEVPKDPDYHFMTDMTNQAIRWVQSVKSLTPDKPFFIYFAPGATHAPHQVPKEWIAK
                     YKGKFDQGWDKLREETLARQIKLGVVPAGTKLAPKPAAIKDWATLSADEKKLFTRQME
                     VFAGFAEYTDTEIGRLVDTIAELGQLDNTLVFYIVGDNGASAEGGMNGMFNEMTYFAG
                     VPETVADVLKHYDELGGPMTYGHYAAGWAVAGDTPFTWTKQVASSYGGTRNGMVVHWP
                     KAIKAKGEIRSQWHHVIDVAPTILAAAGLPEPKVVNGVPQTPIEGVSMLASFNDAKAK
                     DNHLTQYFEIFGNRAIYHDGWLAGTVHRAAWETKPRATLDKDVWELYDTRTDFSLAND
                     LAAKSPAKLKEMQDLFLKEAVKYNVLPIDDRLLERSNAAIAGRPDLMAGRTSLTVYEG
                     MRGMSENVFINAKNRSHTITAEVTIPEKGANGVILCQAGRFGGWSLYLKDGRPTYTYN
                     FLGLQRFTIAATTPVPAGKATIRFEFAYDGGGLGKGGVGTILVNGKKVADGRIERTQF
                     GIFSADEGADVGEDGETPVVEDYGIPAPYRFTGRIAKVTVDVAPMKAPEKAAEGKARA
                     ETAQKRALSD"
     gene            complement(3641..4297)
                     /locus_tag="PLB01_01245"
     CDS             complement(3641..4297)
                     /locus_tag="PLB01_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75954.1"
                     /translation="MTALIVRTCAVAVAVALARSVAFAQAPAEAPVPPAAGANAWSVD
                     ASANTYFVPDANDFVSPVVKADRGALHLEARYNYEGFRSGSIWLGYNVSLGKELTLDL
                     TLMLGGIFGDTAGVAPGWRFALGYGKLELTSESEYVFDSRDSANNFFFNWSELSLSLA
                     DWVRAGLVVQRTRLYGEGRDVQRGFLVGFAWKKAYATAYLFNPDVDKPSFVLTLGAGF
                     "
     gene            complement(4294..4956)
                     /locus_tag="PLB01_01250"
     CDS             complement(4294..4956)
                     /locus_tag="PLB01_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQQ75955.1"
                     /translation="MAEDPFVSSKRLLEPVDRASEILFGLIMVLAITGSFSVAGAGRT
                     EVRTMLFAALGCNLAWGVIDGVFYLMGCLAEAANKIRVWDAMRNTSDPKEARRALAGA
                     LPAVVAAAIQPAELEAMQVRMQALPEPPDRSLLRKHDWLGALGVFLLVFLSTFPVVIP
                     FMFMKDVRLALHVSNGIAIALLFLTGYSFGRHIGRHAVLTGVAMVVFGAALVALTIAL
                     GG"
     gene            complement(5010..5549)
                     /locus_tag="PLB01_01255"
     CDS             complement(5010..5549)
                     /locus_tag="PLB01_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HQQ75956.1"
                     /translation="MKKIFAVILFAMLGAEGFSVLAQQKSLSSSLSIAAFPQVGQSAQ
                     QQSQDEAACYDWAVKNTGTDPFDLQKKAAQQQQQTAQASAQAQAAGEGAGVKGAARGA
                     AGGALIGAVAGDAGTGAAIGAVTGAVVGRGRKRQAQAQAEQVQAQGAQAQQATAAQMT
                     SFKKSFCACMEGKKYIAKY"
     gene            complement(5571..6002)
                     /locus_tag="PLB01_01260"
     CDS             complement(5571..6002)
                     /locus_tag="PLB01_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75957.1"
                     /translation="MSKKPILFCWLVAALLVAGAPDAVADNLTGTNRFLCSVVTVGRC
                     DIDGCMDDSPDGALVPQFVIIDLGAKLLSTTPASGQNRTTPIESLRREKGLVVLQGLE
                     NGRAFSFVIAEKTGSASVAIAREDLVLAVSAMCTPVPPAEK"
     gene            complement(6044..7702)
                     /locus_tag="PLB01_01265"
     CDS             complement(6044..7702)
                     /locus_tag="PLB01_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQQ75958.1"
                     /translation="MTTDRADGGTLRFDLVAGLTAAAVVLPKAMAYATVAGLQVAVGL
                     YTAFVPMAVYALLGSSRALSVSSTTTLGILAGTQLGLAVPDGDPARLVPATATLAALV
                     GAMLLLARLLKLGFVANFISTPVLTGFKAGIGLVILLDQVPKLLGVHVTKQGFFRDVV
                     GVARQVPETSLVTLAVAGATLLVLVGMERLWPHSPAPLVAVGGGIAASWFLGLKALGV
                     STVGLIPRGFPSLTLPDPALLADLAPGALGIALMSFTETIAAGRAFTGREDPPIDANR
                     ELVATGVANLGGAFLGAMPAGGGTSQTAVVRAAGGRTQKASLVTAGTALATMLLLAPL
                     LGLLPNATLAAVVIVYSVGLIQPVEFAAIRRVRTMEFRWAIAAMLGVLVFGTLKGIVV
                     AVILSMIGLAGQTAHPRVSVIVRKRGADVLRPMSPEHPDDETFEGLLILRPEGRLYFA
                     NAQNVGEQIKALVAKHRPRVLALDMSRVPDIEYSALHMLMEGEQRMTESGGIFWLAGL
                     NPGALEVVRKAGLEERLGRERMLFNARVAIERYQALQAEALRDS"
     gene            complement(7714..9741)
                     /locus_tag="PLB01_01270"
     CDS             complement(7714..9741)
                     /locus_tag="PLB01_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75959.1"
                     /translation="MVVARRIALVWLAAPLIAAPPASAFDSKGHVVVEALAYRTLVEG
                     HDGQAPRPEVLRDLINDGALDLPFCFGRGDVPPRECVDAPASNPLLAWPQPLTDRPDA
                     AFRRQFSDVGQCFHYMATLDDAQSDVLPGTAVPRALATSAVVRCNDLLDGLLRQVVVI
                     GGPATRRSSHGLYEFMHAVGDSFSGSHTERAEDGRIGYLRVWKPIEKLVKLPTERAAR
                     IPDASYHKWDDHRDKTYVVEGGDARCEKRTDQPYNVPYECLSPEGDRARRALTELLVL
                     ARELRLAQLAAPKGTDTAPERSDAWRGYKAKWFTPAHPCEGAECAEREKPDPPPGRYA
                     LLGLETIWNPTRDFVDIAARGTLIKYSEDLNPFLYALNASVGYRQYTDGGGGAGLVGL
                     GVDLVLPVGLRATLGFTPAALRVTYGGDRAGPELVTRFFRASYKLKDGLFLTIDAPME
                     VNWLKPRVEWMFGVGLIYAPPSSKLSKDRGLLLSKETLAVRQDGAWVPPPAPYGHIEG
                     RRGSWYVVSGVTVAETPANAVEGRHYGLGLLGGEVAWDRNRWGGRFEWVPALSVAVAN
                     RVTSGESKYLTGTLAASIRWYALKVLGLSLTPVRVEYGPKVRGDEEVDTSPGVHGKPG
                     STYYFQAGTRLGVMLNAGIIDILVEGPTLAWDSSPFAAKEILSFRLGIRLN"
     gene            complement(9778..10629)
                     /locus_tag="PLB01_01275"
     CDS             complement(9778..10629)
                     /locus_tag="PLB01_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75960.1"
                     /translation="MKLFRSAGILAAAAMLALAAPAAAQSYAAVKGMDERFRLDLGGF
                     FQNFETTLRLDSAALGRGTEINFEDDLGQDANKTSFRASGYWRFGRHGRFDFGVITWN
                     RSATHTIAKDIQFGDHVYRAGATIDSNLRATQVDAYYAYSFVNTGEAELGLKLGFSAL
                     INSTEFNGSGSITGPGGTVSGSRASDSRSVVAPVPAIGAYGRFTLLPGLMISAQARGL
                     PTVTISGYSGSFVDAGAYLDWYPWKNFGFGGGYSYTKITFEREQDPTIRFEYTYSGPI
                     VYVSLAF"
     gene            complement(10725..12086)
                     /locus_tag="PLB01_01280"
     CDS             complement(10725..12086)
                     /locus_tag="PLB01_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQQ75961.1"
                     /translation="MKSRFSLPRVLACAAVVALLAAGSAFATEFNLNAVTFPDGTTID
                     LAMARTNATPNAARLDAAVRYKEGQAKVEVSFKKMEPAILFGGDVSSYVVWAITRDGA
                     VENLGELIVNESNASGSGQYQTGKKSFGLIVTAEPFSLVGRPSEYVIATSGPADPKKA
                     PGSTFAFSNFRTGIVKADTNTIAGLTYKDKKPVAIVQAERAVALADRTGAFSLNPKAA
                     EEAKRALVQAQNSAAGGSKKVINDYSSRAVFLSSEALRDYGRKLEADAEAAKKAALAE
                     SQMTVAQLKEAQAKLETDKARLLADQAQLQKEKAELEGRLKNSMSSIMETRDSARGAV
                     MSLPGISFETGKAALKPSAQLTLAKLAGIAQVFPAVNMRVEGYTDNTGSAATNQKLSE
                     ARAKAVYDFLKAQGVADTRLAFQGLGPASPVADNATAEGRAKNRRVEIVAAVGEIKPV
                     DAN"
     gene            complement(12176..12724)
                     /locus_tag="PLB01_01285"
     CDS             complement(12176..12724)
                     /locus_tag="PLB01_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQQ75962.1"
                     /translation="MRTPFGTLVLAAAVVSAVGHASSITVESKADPAADFSRYKSYSW
                     TEMKEVRSEAVKKRIMSAVDAQLQRKGFVRTDGGGDLKVAVHPSLSKEYPTNSYDPGW
                     EYGWGTWAPPFGDIGTGSALGASIPIGTVMVDLVDASTKKLVWRGAARSPLDSEASAE
                     KRQELVDDAMKKLFKTFPSKKK"
     gene            complement(12737..13393)
                     /locus_tag="PLB01_01290"
     CDS             complement(12737..13393)
                     /locus_tag="PLB01_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQQ75963.1"
                     /translation="MKFSFHRLTFAALLVLAAAAIPASAQNVPGTWELTPYGGVMFGQ
                     QIFQGGSTTVDVDNAGTFGGRLAYNVTPAFGVEFGYGHTSANLNATNYKGISGGSGKI
                     GHLAQDSFEIDALWHWGSPRASGYFLFGGGLMSMNPSVDGAATSSSSNFTWSLGLGGK
                     WSITPSAAIRVEGRFRSTNLGNTTSQGVWCDYYYCYAYSTSWYSNGEITGGVTFRFGK
                     "
     gene            complement(13446..14276)
                     /locus_tag="PLB01_01295"
     CDS             complement(13446..14276)
                     /locus_tag="PLB01_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HQQ75964.1"
                     /translation="MNSRVSLGAFPVLASALFFSACSSNAPTTSVTPAPPAVQERNWG
                     YVANAETAGPSDWVSLPGNAACAAGKKQSPVDLATRAPFPVEAKDLPNLVFRYGTTKL
                     HLVNKGHTVQADVEKGSTVEIDGAAWALLQLHFHAPSEHSLDGLHYPMEMHLVHAGPD
                     GKPGLVVGVFLVQGGDNPAFTPLLSDLPKVKGARKDDSSVTVDLAKLLPENRAYLAYD
                     GSLTTPPCTEGIRWYVLQSPMGITGEQLGAFVTVPGMNTTNRPVQPLAGRKVLLDTTP
                     "
     gene            complement(14345..17515)
                     /locus_tag="PLB01_01300"
     CDS             complement(14345..17515)
                     /locus_tag="PLB01_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HQQ75965.1"
                     /translation="MKSAVRRHPVAASVFAVVLLAAAGVALAPWWIDLGPVRARIESA
                     ASSALAGAVRYERMDLAWFPRTEAVLRSATVSVPRTVHGRVRTVRVAFAFLPLLRGRF
                     VPSRILVEGADVFLSRRDGRGLWVEGVKVEGSFRSFEGGTDIVLSRLSVDSPALTASG
                     NFRWDPVTPRADVSVRGSLDGGAVRELLLAFAGDDRTIAQIFAIFRGGSLTSIAFGSV
                     ASSPSDLFALERMRIRAKVEDARVFVEGPDLDLRDVSADVALEGGVLTVERASARLGK
                     SKAEGGTVLVGLAPGDGRLRVEAQVKADLAEVAAILGRAIPDRSFREELALVEGLEGR
                     ATGRIAIGECAGDLNTGVSVSDLRFSANYRRIPWPLRVDRGEFAFDGKRVGASRLSGS
                     LGHSTFSGLAARVRLGRSAQLENALGSIEVSLEDFLEGVRKKPPADALAKSVAHLSGT
                     VRVDVRRVSGPLARLDEASVDASGTFKEVLVSSPSLPTLSIPSGRFAVGDDAVRVSDA
                     AIRTLDASLLASGVWKGWRGGERSLEAAANGTIGPDAVRWGWGRASLPAELLPAAPIA
                     LNRVRVALARGGAFSASGDLVVAGGPRLALDVAGDGKKVDIRNLTVADGDATASMALL
                     RPDAAFDVRFKGRLALSTVEKLFAEGRRHPGGIEGDFRALVASENLGRSTAEGTLTAT
                     GFEVPTPAGPVTIERADLRAAKNRFDVASSSFVLDEQRFSAAGSATLGDGAIALDMDV
                     GTGDLAWSRIEKILDRVAQGKKKESGEAARETAARAPLAIRGDLRVSLDSFSYEDLVW
                     KPVLADLKFGPDSLAARLRKAEICGISATGEARFLPGGAMAVDVRADAAGPDINVPLT
                     CFGFENTRMTGVYEASVQVRGEGPASELPRALRGPLRFKTAKGRIGKATLLTRILGVL
                     NATDVFAGKSGGRVGDAIPYDEITLNGDLGDGRASIREAALKSPSITMAATGTVGYLD
                     RSLDLMVLSHPLSTLDKVVQAVPVVNTILGRNFLAVAVKVTGSMAEPKVALTPGRDVG
                     KGLVGILERTVTLPVTVFDPPAKPIP"
     gene            complement(17570..18598)
                     /gene="glsA"
                     /locus_tag="PLB01_01305"
     CDS             complement(17570..18598)
                     /gene="glsA"
                     /locus_tag="PLB01_01305"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HQQ75966.1"
                     /translation="MSVVKKALAAAAALALAASLAPAASAQTPDDIQKAVNAAYEKYK
                     DIKEGKNADYIPALAKVDPKIYGIALVTTDGKIYTAGDLKSEVSIQSISKVFTMAKVA
                     EESGFDAIVKTIGVDATGMRFNSIVSVEFAKKALGGPEINPLVNPGAITATSMLKGKD
                     RAEIWNGLLATYSDFAGRPLTVNQDVFKSESDTNQRNQAIGYLMYAYEYIKKDPLRAT
                     DIYTEQCSVNVNAKDLAVMAATLANGGRNPLTKKQVMKTENVPGTLAVMATAGLYDDS
                     GKWFYRTGLPGKSGVGGGLIAVAPGKFGIAVISPPLDDAGNSVRGQKAIADIASALKA
                     NPLASTPR"
     gene            complement(18595..19815)
                     /locus_tag="PLB01_01310"
     CDS             complement(18595..19815)
                     /locus_tag="PLB01_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HQQ75967.1"
                     /translation="MRGTSLRTIAIAALALGALTARAQTDADKPAGPRMEIYGFAQLD
                     MGYDFKQVNPDWFDVLRVTKLPSYENQFGEDGKTYASVRQSRFGVKGWVPTSVGEIKT
                     IFEFEMFGVGVDAGQTTIRLRHAWGELGQVGAGQTWSPFMDPDVFPNQLEYWGPSGMV
                     LFRNVQVRWMPMQGENEVFLALERPGASADGGNYADRVELQNVKIRTQYPDISAHYKK
                     SGKWGHVQLAGMLRFIKWDDLNVDAVDLSGSATGWGLNLSGIINVTENDVIRSSVVYG
                     EGIQNYMNDAPIDIGVKNNFGNPTKPIEGKALPVLGIVAFLDHNWNKKFSTAIGYSYL
                     NIDNTDGQKASDFQQGHYALANLLYYPASGVMAGGEFQWGKRKNFSDGFSVDDYRIQF
                     SFKYNFGWKLGGKS"
     gene            complement(19848..20894)
                     /locus_tag="PLB01_01315"
     CDS             complement(19848..20894)
                     /locus_tag="PLB01_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ75968.1"
                     /translation="MVRLAFSLAFASGASSLRAVEAPLFHDAHFHLTNYVQEGPDVRD
                     FLKIMGTKVGRSTLFGIPLQQTWSYGNSGDFAPTYYLHTDAPLYYYSFTDAFIAMAFR
                     SLTKEQQARFDPMITGFNPADMYAADHIRRVLATFPGVFVGIGEFTIHKEFVSSKIPG
                     ETASLTNPALDRILDFGAETGLLVILHNDLDMPFARDGTPPVYLEQLKALFRRHPKTT
                     IIWAHLGLGRIVHPLKQYVAFVTAILDDPTLAHVSFDISWDEVAKYAVATPEALQNTA
                     GALNRHPDRFLFGTDALSPTTPEGYFKTWEAYTPLWAALTPEAREKVLKGNYERLFDA
                     ARVRVRVWERANVK"
     gene            complement(20946..21212)
                     /locus_tag="PLB01_01320"
     CDS             complement(20946..21212)
                     /locus_tag="PLB01_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75969.1"
                     /translation="MLRIAEERLGDLTVVRVAGRLGGAGVEELERVCAASLRPLRIDL
                     AALLQADELGIALLRSLRGSGAELTGVSPFIGLLLDGGASRAAG"
     gene            complement(21375..22076)
                     /locus_tag="PLB01_01325"
     CDS             complement(21375..22076)
                     /locus_tag="PLB01_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75970.1"
                     /translation="MKLRVASLAVFVSFAPALEGQQPAASAPTAAPAKEPRIIVYRID
                     LVPNGSMFAMNEPVLDGDTYVFRALPERTEMRLPKVKVKSVARWSTDYSKEVVWRVEL
                     AGAAGMHLARNEPVKKGKNWVAYTWKNNTLVSVPETDVVKITRLTGAEAFKAEQLALG
                     LIVLEGESMTPGFKGGNAPVNTAPASGSAAPAGNPAGPGNWTYQGQPGASDAYAPAGG
                     TVSKPGDVPMMPTPR"
     gene            complement(22118..23284)
                     /locus_tag="PLB01_01330"
     CDS             complement(22118..23284)
                     /locus_tag="PLB01_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQQ75971.1"
                     /translation="MRRGAVVALVVAAVLALAPAVFAQAPPGDDSAPAAKPSLFKDPE
                     DGAFDVGPWAATRTGILPIPVPITEPAVGYGLALGLLKIHGGGLAGTKDAPLGVTGKP
                     VPPDVSALAGAATENGTWMAAVAHVGYWGGDRWRYTGVVGRMSPQLDTYDAQGRAYGF
                     NLDGWVVFQELKRRVGRSNLFLGARYIFMDTTTRFDSGLAPPDVPAPEFGNRDSGLGA
                     VAEFDSRDNTFTPNRGVQFKASGTLYGPYLGGDHDYQRYTGDGLFFWDVHPRLVLSTR
                     LRTQAVAGDAPFYARPFVRLRGIPAMRYQGETAVSLDGEARWAVTKRWWLAVFAGAGW
                     TDAGSARVLVDESVHAGGAGFRYLVARKLGILAGLDVAKGPEQWALYVVFGSSW"
     gene            complement(23284..25185)
                     /locus_tag="PLB01_01335"
     CDS             complement(23284..25185)
                     /locus_tag="PLB01_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75972.1"
                     /translation="MSRGRTFAALLLVAVSAAPPARAWIYPEHRNIAGSAFEGLDPAR
                     KAVLEKLWGEARKGHEDRLCEKPWAGDQGNKPGCIDLAAFPALAGDHSCSADQLLKTV
                     LETKWVLDVAAVTSVLDKGLATAKNKNEARNRLTKSDLDLQRKDPEYATRAGSNNVHF
                     LLTRDTDDVKEFLLASLTKDVELNGLGTWARYHIAAMRLVYELEAGRVPEDKRSEVAL
                     TALAAEAFGVHFLEDAFASGHVAGTWGDVATRKGTHDYYNRNGFDAVTWNGKPILMFG
                     DANMKPADLARASAAVRESFNQVLDAASPGWALAADVSAIPIDWAKGVPGFDTCKTMK
                     MPAAGEIPLPVVKEAASILLDTPKPGRKPPEGALPRFRSEIGPFIGLAAGISAGWADG
                     GFESTESTNRANGTMEIGARAGLGLEDVLGEAGDGQIFLQAGVLQEIGSKDVCADPCS
                     TSGLVANLALRTPARKGIATRIRMPFWLIPGDLILAAPILGFLAPKTYESMAMVAASG
                     GVIPWQTGLSTFLGRVQFVLGREVGATFFGYSGGEDQLFVSAPGSGTALTAVSLRSIR
                     WDVPIVEVRPFRSYATTQAAAVLIQIGAAFDKPTRVTVMAPVGAPAPDLKTATSVYFR
                     LAFDWRQYF"
     gene            complement(25188..26057)
                     /locus_tag="PLB01_01340"
     CDS             complement(25188..26057)
                     /locus_tag="PLB01_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75973.1"
                     /translation="MKRTSCALALGLAVLTFSPAAAGADVAFSEFPEAADKPVVIQLA
                     GVWNSFDTQARLDVTRGGVAAIGTTLDLEQVFGVPSAQWDFRGDGSWRFSKRNYLDFG
                     YSSLNRSGTRALDADVVWNGNTYKAGVTVDGKFDNNYVYVGWHYDIFRADNVKVWAGL
                     SIAYEHFETGLNGLATITNPDGTITKSAVAADYSIGLPAPLVGLGVSGAISNHWTFDF
                     YTRAIGFSSTDIAGGILEAGLSFAWYPTKNFGAVFGADMTKITIKKYRKDNETLSAEY
                     RYTGPRVGLVFGF"
     gene            26231..27793
                     /locus_tag="PLB01_01345"
     CDS             26231..27793
                     /locus_tag="PLB01_01345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQQ75974.1"
                     /translation="MASVMTAEALEFESTLAEVTARLLAAPGDGFETELDHAIERLAL
                     FFGADRGSILLFEDDGTTNVVAAFAGPTLLTVPKGPTQLLPNWAGQARRGLGFTMHRP
                     DEIPESWTEERAFVAANGIQSNLMFPLRIGEKPIGLLSLSSLTRGRDWSASFLTRFRL
                     FGEILASAIVRARAERDLRASLAEISKLKERADAENVVLREEVRDAQGFDEIVGRSPA
                     LMQVLYMVEQVAPTGSAVLLTGETGTGKELLARAIHRRSSREGRPFVAVNCAALPGTL
                     IESELFGYERGAFTGALQRRLGRFEVATGGTIFLDEISDIPADIQGRLLRVLQEGTFE
                     RLGSSHTIRTDVRVVAATNRDLEAAVAEGRWRADLFYRLKVFPIEVPPLRHRREDIPL
                     LVWYFVTRKRTSLGRAVTRIPDKAMSAFQRYDWPGNVRELENVIERALILSPGDTLVV
                     DERTFAVPSKTRAESGIWSLEDVERGHILKVLGECGWRIAGRGNAAERLGMNRSTLRS
                     RMEKLGIERPQA"
     gene            27790..29304
                     /locus_tag="PLB01_01350"
     CDS             27790..29304
                     /locus_tag="PLB01_01350"
                     /inference="COORDINATES: protein motif:HMM:NF039990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HQQ75975.1"
                     /translation="MRRFAVLAAAFVVFSGSLASAQENGKEKEISSSERAQAAASAQE
                     ALPAQTVAPPKTPSKKSSSSSSPSSSSEIEELKAQIAKQEETIRALEERLAKLEALTN
                     ASQASAGLEERLKQLETEVKKLPEAEDVVSVGEFPGSFRIPGTDAALKLGGQVRVTAV
                     ESLDAIGTDDRFVTSSIPVEGSEAAGKGARTTLAANASRFNFDFRTPTGVGAMRAFIE
                     VGFDINSNAALRHAFGQWDHWILGQTWSTFSDPEAEPFGIDNEGLNAISLFRQPQIRY
                     TTTFRDAYTIAASIENPAPDVTNASGVNLIPDFVLRARWDPGKGKGSLPVLGQLGHVQ
                     LAVLLRQIRAESDLDPNQTASRGGLGLGLSGVLRTGWWAETDDVKFSLYGGNGIGRYI
                     TDLRAAGGQDAYFDAATGEMTVLGVTAAYLGYEHAWSNTLRSTMTMGWVWVNVLENQP
                     NDALKYTRRFSLNLAWSPITRLDLVAEFLTGTRVNKDLQSGTATQIQLGSRFRF"
     gene            29309..29728
                     /locus_tag="PLB01_01355"
     CDS             29309..29728
                     /locus_tag="PLB01_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQQ75976.1"
                     /translation="MRRLIHKTASVWTKEEGAALLLPLLVASIFVAPLLRDWFPVLAA
                     LGTAWAYAYDLLEVVRPGSFQAARSVTEGSYPPLLYYSFVTLTTVGYGDVTPLSSAAR
                     ALSNLESLIGVLFPAVLIGRLLSKQVAGRPPEPPAPG"
     gene            complement(29730..30800)
                     /gene="ydiK"
                     /locus_tag="PLB01_01360"
     CDS             complement(29730..30800)
                     /gene="ydiK"
                     /locus_tag="PLB01_01360"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HQQ75977.1"
                     /translation="MNDRRPDLPQIVFAILIICGLLALSLWIVAPFAAALVWATTIVV
                     ATWPVLLQLEKWFGGRRGPAVAVLTIALLLVVVVPFLAGLGTLLGNREAIAAGIDRFR
                     TAEVPPPPSWVAGIPLVGTQAAAAWQDLAGGAVHDLAQKGAPYARQALNWFMSTLGSI
                     GGLFFHLLMTVIVAAVLYSLGDAAADRVRRFARRLAGDRGDNVVVLAGQAIRGVALGV
                     VVTAVGQSVLAGVGLAIAGVPFAGVLTVVALVLCIAQVGPILVLLPAIIWLYGSGQTG
                     WGTFLLVWSIPVVTLDNVVRPILIKKGADLPLLLIFAGVIGGLVSFGLIGIFVGPVVL
                     AVSHTLLEAWIDAGDEPQPAAE"
     gene            complement(30831..31400)
                     /locus_tag="PLB01_01365"
     CDS             complement(30831..31400)
                     /locus_tag="PLB01_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HQQ75978.1"
                     /translation="MSGALAGGRKSLDLFGVFVVAVVTAIGGGTLRDLLLGRLPVFWV
                     REPEYLGVVAAATAATILFTRLRMPKENRLLAADALGLALFSVTGAQIAETATDAKLV
                     IVLMGAVTAAAGGMLRDVLCGDVPMILHGRELYATAAAAGAAVYVGLSAAGAGRPLAA
                     GAGLLTTLLLRLFSLRYGLGLPAYHLPED"
     gene            complement(31476..32573)
                     /locus_tag="PLB01_01370"
     CDS             complement(31476..32573)
                     /locus_tag="PLB01_01370"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HQQ75979.1"
                     /translation="MSPKRIVSLSLVLLVALLATPGASAQTAAAPAAKPALAHVVILA
                     TGGTIAGVQPKEGEPGYKSGSLSIDALIKGAPGIEKLARLDGEQIASIGSQDMNDVVW
                     GKLAKRANELLAQPDVDGIVVTHGTDTLEETGFFLGLVLKSNKPVVLVGSMRPATSQS
                     ADGPLNLYNAVAVAADPASQGRGVMAVVNDEVHTGRDVHKTHTTAVQTFVSTTRGTIG
                     KVYYGKVRYGYVPTYKHTVTSEFATAPRSIYPPRVDIVFAHEGCDGTFVKAAVAAGAK
                     GIVLAGVGDGNATADMINALSEAAKSGIVVVRTSHVGFGMVRRNVELDDDKLGFVAGM
                     DLNPQKARVLLRVALTQTNDVQKIQKYFEEY"
     gene            complement(32584..34296)
                     /gene="aspT"
                     /locus_tag="PLB01_01375"
     CDS             complement(32584..34296)
                     /gene="aspT"
                     /locus_tag="PLB01_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0070906 - aspartate:alanine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HQQ75980.1"
                     /translation="MHDIAESLRQNPELAVFLTLALGFLVGRLKIGTFKLGNVVGTLL
                     AGVVVGQIGVKVDPVVKLVFFDLFLFATGYKVGPQFFRGLKKNALTQVSLTLVLCVTS
                     LVTTVAAAKIFKYDCGTAAGLMAGAFTESTVIGTAGETINRLPISDAEKKRLLDQIPV
                     AYAVSYLVGTGFVVWFLSGLAPKLLKVNLKEEAKKLEATLSGGTGEKREEGTQSAYRQ
                     WDIRAYELPPQWAGKTIGEFERSFAPDRVFVKRVRRGKEEKDFLEGEEGIALAAGDVL
                     ALAARRPVFLSKKSSSSSSSSTFGVEVEDRQLLDWPIVVADAVVTSRDIEGKTLAELA
                     EAHGRGVGLVSLVRGGEDVPVSLHTTVDRGDLLRIAGAPEDVARTGKAIGYVERPSSE
                     TDVVFVGLGIVLGGFVGLLSVTVGGLPLSLTASGGALIMGLVFGWLRSVRPTFGRIPE
                     PALWVFDTVGLAVFIGIVGLSAGPSFVSGIKETGPSLLFVGLVVAVTPHIAALFFGKY
                     VLKMNPVLLFGACAGAGTVTAALRGIQDEAGSKLPVLGYTVPYAIGNILLTAWGPVIV
                     MLTK"
     gene            complement(34311..35717)
                     /locus_tag="PLB01_01380"
     CDS             complement(34311..35717)
                     /locus_tag="PLB01_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011980664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HQQ75981.1"
                     /translation="MSPAAKTWVWRGVTIALGAAIWFWPVPEGLTAQAWRLFAIFAAT
                     IFSVIVGAFPILTASVLAVAVSVLSGVLKPAKAYAGFANGTILLIVVAFLVARAIVKC
                     GLGQRLGHLVVSLFGKSTLGLGYSVFFLDAVIAPAFPSDTARSGVLYPLVLSLAEGSG
                     SKPDEATRKKTGAYLMFCGMATLSLSSALWLTAMAGNPLGAEIGKSLGLSVTFGSWLV
                     ASIVPTLVCLAVLPYLIYKVFPPELKATPEAPAAAKKALAAMGPLGLQEKIVAVTFVG
                     MVTLWALAGTLKVDLTAVAFLGLGIFLATGVLTLKDIGQQGDVLATFIWFAILFAISG
                     QLNTLGFMGWIGQRLAAALGGLPWLAVYVLLVLAYVLIHYLFVSQTAHVLALFGVFLG
                     VGVQLGVPVAPLGFALLISTSYLSTLTPQASSANLLVVGSGYLTQGELYKLGTITTAF
                     NVLVLLLIGTPWLLFVTR"
     gene            complement(35714..36235)
                     /locus_tag="PLB01_01385"
     CDS             complement(35714..36235)
                     /locus_tag="PLB01_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75982.1"
                     /translation="MRSRRTALLAVAGAAAYLLTSAAAAEEWRPFSATWALAGKRTSL
                     PTEGARRAAIVRATGSFVVTKGDVVGRGFFGELIGYDDGGTLLVGRAVFTDEKGEKVF
                     ATLKAQPLGTGRTAAATITGGTGRWAGLEGEFTFAWKVVVESGEDDFDAMTLNVEGRA
                     RRAPPKTPAEAQK"
     gene            complement(36232..37647)
                     /locus_tag="PLB01_01390"
     CDS             complement(36232..37647)
                     /locus_tag="PLB01_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HQQ75983.1"
                     /translation="MSKRATRLEHDLLGDLAVPADAYYGVQTARARENFHISGVELRL
                     YPDLIKAFAMVKLAAARANFDCGQFDKKILKGIEGACEEIIAGKLHDEFRLDVFQGGA
                     GTSTNMNANEVIANRALELMGKKKGDYEACDPHDHVNCSQSTNDAYPTSLHVGMALGN
                     VRLVAAMRELIAAFRKKGKEFAPILKMGRTQLQDAVPMTLGQEFNAFAQTLEGEVLAL
                     ERIQSVLCETNMGATAIGTGLNAPEGYAEKCTKHLAKITGFPITLAPDLIEATQDTQA
                     FVLYSACMKSLAIKLSKVCNDLRLLSSGPRCGLREINLPAKQPGSSIMPGKVNPVIPE
                     VVNMVCFRVIGSDLTVSMSAEAGQLQLNVFEPVIAACIFEAQTMFINAARTLRVHCVD
                     GITANPDVMKHYVEYSIGTVTALNPIIGYDKATALAAEAMKTGAGIMELIRRKKVLTE
                     KQIAEVLDPAAMTGQKRTGKK"
     gene            37915..>38946
                     /locus_tag="PLB01_01395"
     CDS             37915..>38946
                     /locus_tag="PLB01_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HQQ75984.1"
                     /translation="MKRTNNANGLLGRGWRSAIAIACALLLVPGEGILLAQEASPPAA
                     AAEADDPPLTADQLDSLVAPIALYPDDLLAQVLAASTYPLELMQLQQWMAKHKDLKDK
                     ALADAVAKENWDPSVKSMAAVPDALKRLTDDIQWTTDLGNAFLDQQKDVMDSCQRMRK
                     KAKDKGALASNEQMKVETKVVETKEVIVVQSANPEVIYVPSYNPVYVYPPPVYPYPPV
                     YYPPYTPGAAFFSFSVGVMVGAAFWGGSCCHCGWGGGNVNINVNNNFNRNTNINAGNR
                     GQGGGSWNHNPSHRGGTPYGNKASASKYGGQTRGQQASTRQQGGRGTSGAGDRAGGSR
                     GGPSAGTSDR"
BASE COUNT         6167 a        13249 c        13426 g         6104 t
ORIGIN      
        1 gcgaaggcga gcagctcccg cggcgcctcg agcatgggcg gcggcggcgg atcccgcggc
       61 ggcggcggcg gacgcggagg acgccggtga ggggagagac acagatgaag acgaagaatt
      121 ccttcgaatg tgaatgcgcg cgggcgcgcg gcgtcctctt cggcctgctc ctcgctctcc
      181 ccctgcttct tgccttcgaa ggcaatcttt cggcggcagc ggccgcgccg aagcagaaga
      241 gcttctcctc gccgaaggcc gccgcggagg cgctgatctc cgcggccgcg cggttcgacg
      301 tcccggccct caccgagatc ctcgggccgg acggcgccga cctcgtcgtc tcgaaggacc
      361 ccgtgcagga ccggaacacg gcagcggcgt tcgcgaggaa ggccctcgag aagcactccg
      421 tcgtcacgga ccccgggaac gcgggccgcg cgacgctcgt cgtcggcgac gagggttggc
      481 cgtcgccgat cccgatcgtc cggaagggcg gcaggtggct cttcgacacg aaggccggcc
      541 ggcaggagat tctctaccgc cgcatcggga ggaacgagct ggacgccatc gaggtctgcc
      601 gcgatttcgt cagggcgcag cacgcgtacg cgctccagaa gcacgacggc tcccgcgtga
      661 accagtacgc gcagaagatc atcagcaccc ccgggaagca ggacgggctc gcgtggcgcg
      721 ggcccgacgg ggcgccggcc ggacccctcg gagacacgat cgccgaggcg atcgccgcgg
      781 gctattcgaa gaagtccgag ccgcttcacg gctacacctt caaggtgctg aagggccagg
      841 gcccggcggc gccgctcggc gagatggact tcgtcatcga gggcgcgatg atcggcggct
      901 tcgccctcgt cgcggcgccg gccgactacc gcgtgaccgg cgtgaagacg ttcatcgtga
      961 gccacaccgg cgtcgtctac gagaaggacc tcgggccgaa gacgctcgag cagttccagg
     1021 cgatggagcg ctacaacccg gacaagacgt ggaggccggt caaggagccg tgaacaagtc
     1081 ctccgcgccg ccgttcggcg cggaggcacc tgccgcgggt ctcagtccga aagcgctctc
     1141 ttctgcgccg tctccgcgcg agccttgcct tccgccgcct tctccggcgc cttcatcggc
     1201 gccacgtcga cggtgacctt cgcgatcctg cccgtaaacc tgtagggcgc cggaatcccg
     1261 tagtcctcga cgaccggcgt ctccccgtcc tcgccgacgt cggcgccttc gtcggccgag
     1321 aagatcccga actgcgtccg ttcgatccgg ccatccgcga ctttcttccc gttgacgagg
     1381 atcgtcccga cgccgccctt gccgaggccg cctccgtcat aggcgaactc gaagcggatc
     1441 gtcgccttcc ccgcgggcac gggcgtcgtc gcggcgatgg tgaaccgctg cagcccgagg
     1501 aagttgtacg tgtaggtcgg ccggccgtcc ttcaggtaga ggctccagcc gccaaaccgt
     1561 ccggcctggc agaggatcac gccgttcgct cccttttccg ggatcgtcac ctcggcggtg
     1621 atcgtgtgtg agcggttctt cgcgttgatg aagacgttct cggacatgcc gcgcatgccc
     1681 tcgtagaccg tgagcgacgt gcgtcccgcc atcaggtccg gccggccggc gatcgcggcg
     1741 ttcgaccgct cgagcagacg atcgtcgatg ggcagcacgt tgtatttcac ggcctccttc
     1801 aggaagaggt cctgcatctc cttcagcttc gccgggctct tcgccgccag gtcgttggcg
     1861 aggctgaagt cggtccgcgt gtcgtagagc tcccagacgt ccttgtcgag agtcgcccgc
     1921 ggcttcgtct cccaggccgc ccggtggacc gttccggcca gccatccgtc gtggtagatc
     1981 gcgcggttgc cgaagatctc gaagtactgc gtcaggtggt tgtccttcgc cttcgcgtcg
     2041 ttgaacgaag cgagcatgct cacgccctcg atcggtgtct gggggacgcc gttcacgacc
     2101 ttcggctcgg gcaggcccgc cgccgcgagg atcgtcgggg cgacgtcgat gacgtggtgc
     2161 cactgtgaac gaatttcgcc cttggccttg atcgccttcg gccagtggac gaccatcccg
     2221 ttgcgcgttc cgccgtagct cgaggcgacc tgcttggtcc aggtgaacgg cgtatcccct
     2281 gccacagccc atcccgcggc gtagtgcccg taggtcatcg ggccgcccag ctcgtcgtag
     2341 tgcttcagga cgtccgcgac ggtctccggc acgccggcga aataggtcat ctcgttgaac
     2401 atgccgttca tcccgccctc ggcgctcgcc ccgttgtcgc ccacgatgta gaagacgagc
     2461 gtgttgtcga gctggccgag ttccgcgatc gtgtcgacga ggcgtccgat ctcggtgtcc
     2521 gtgtactcgg cgaagccggc gaagacctcc atctgacggg tgaagagctt cttctcgtcg
     2581 gccgaaagcg ttgcccagtc cttgatggcg gccggtttcg gggcgagctt cgtcccggca
     2641 ggcacgacgc cgagcttgat ctggcgggcg agcgtctcct cgcgcagctt gtcccacccc
     2701 tggtcgaact tgcccttgta cttcgcgatc cactccttcg gaacctggtg cggcgcgtgc
     2761 gtcgcgcccg gtgcgaagta gatgaagaag ggcttgtccg gggtcaggga cttgaccgac
     2821 tggacccacc ggatcgcctg gttcgtcatg tccgtcatga agtggtaatc ggggtccttc
     2881 gggacctcga ccttgaccat gccgtcgtag acggcgggcg cccactggtt cgtttcgccg
     2941 cccatgaagc cgtagaactt gtcgaagccg ttgcgcgtcg gccagcggtc cgtcggcccg
     3001 gacggactga cctcccacgc agcggtctcg tggttcttcc cgaagtgcgc cgtcgtgtag
     3061 ccgttgtaac ggagcatcgc agccagagga gcgacggcct ccgggcgctg gcccgtgttg
     3121 ccgggatacg ccgtcgaggt ctccatgatc gaccccgtgt tcgtcatgtg gtggttgcgc
     3181 ccggagagca gcgccgcccg gctcggtgag cacagggccg tcgtgtggaa ctggttgtac
     3241 ttgaggccgc tctgagcgag gcggtccacg gtcggcatgt tcaggggtcc gccgaacgcg
     3301 ccggactggc cgaaccccat gtcgtcgatc agaacgatga ggacgttcgg ggcgccggcc
     3361 ggcgccttga cctcgaagcg gggaggcggg ggcggcgcgt ttcggacgtc gagaacggtg
     3421 ctgtgcgggt attggggctc gtggatcgga agcacggtcc ggtccgggac aggctgcgcg
     3481 ccgagccgag gggcgcagac gagcgatagc gcgatgcaga atccggcccg cgtggagagc
     3541 tttcgcatgg agttcctttc ctgcggatct cgagggagca gaacggcgaa ctcaaggatg
     3601 ctctcggcgc gtcgcagcgc ctcggatgca cttgcatcag tcagaagccc gcgccgagcg
     3661 tcagcacgaa cgagggtttg tcgacgtccg ggttgaagag gtatgcggtg gcgtacgcct
     3721 tcttccaggc aaaccccacg aggaacccgc gctgcacgtc gcggccctct ccgtaaagcc
     3781 tggttcgctg caccaccagg ccggcacgca cccagtccgc cagcgagagg ctcagctcgg
     3841 accagttgaa gaagaagttg ttcgccgaat cccggctgtc gaagacgtat tcgctctcgc
     3901 tcgtgagctc gagcttcccg tacccgagtg cgaacctcca gccgggggcg acgcccgcgg
     3961 tgtctccgaa gatgccgccg agcatcagcg tcagatccag cgtcagctcc ttgccgaggc
     4021 tgacgttgta gccgagccag atcgagcccg accggaagcc ctcgtagttg taccgggcct
     4081 cgagatgcag cgcgccgcga tccgccttga cgacgggcga cacgaagtcg ttcgcgtccg
     4141 gcacgaagta ggtgttggcg gacgcatcga cggaccacgc gttcgcgccc gccgccgggg
     4201 ggacaggcgc ctccgcgggg gcctgggcga acgcgacgga cctcgccaga gccacggcga
     4261 ccgcaacggc acaggtccga acgatcaggg ccgtcatcct ccgagcgcga tcgtgagggc
     4321 cacgagggcc gccccgaaga cgaccatcgc gactcccgtc aggactgcgt gacgtccgat
     4381 gtggcgcccg aacgaatagc ccgtcaggaa cagcagggcg atggcgatcc cgttggagac
     4441 gtgcagcgcc agccggacgt ccttcatgaa catgaacgga atgaccaccg ggaacgtgga
     4501 caggaacacg aggagaaaaa cgcccaacgc acccaaccaa tcgtgctttc gcaaaagtga
     4561 gcgatcgggc ggctcgggaa gcgcctgcat ccgaacctgc atcgcctcca gctccgcggg
     4621 ctgtatggcg gcggcgacca cagcgggcag cgccccggca agggcccgtc gagcttcctt
     4681 cgggtcactc gtgttgcgca tggcgtccca gaccctgatc ttgttcgccg cctccgccag
     4741 acaccccatc aggtagaaca caccgtcgat cacgccccag gcgaggttgc acccgagggc
     4801 cgcgaagagc atggtgcgca cttcggtccg gccggcgccg gccacgctga acgagcccgt
     4861 gatggccagg accatgatca gtccgaagag gatctcggac gcgcggtcga ccggctccag
     4921 caggcgtttg gacgatacga acgggtcctc agccacggca gttccgtcgt tccccatgcc
     4981 gcgatcgtac gtcagcgctc tgcgcgaact cagtacttcg cgatgtactt cttcccttcc
     5041 atgcatgcgc agaaggactt cttgaagctg gtcatctgcg ccgccgtggc ctgctgagcc
     5101 tgcgcgccct gcgcctgcac ctgctccgcc tgagcctggg cctgcctctt tcttccgcgc
     5161 cccacgacgg ctccggtcac cgcgccgatg gcagcccccg tcccggcgtc gccggccacc
     5221 gctccgatga gcgcgccccc tgcggcgccc ctcgctgcgc ccttgacgcc agcgccctcg
     5281 ccggccgcct gggcctgcgc cgaggcctgg gcagtctgct gctgttgctg cgcggccttc
     5341 ttctgaaggt cgaacgggtc ggtccccgtg ttcttgaccg cccagtcgta acaagccgcc
     5401 tcgtcctgcg actgctgctg ggcgctctgc ccgacctgcg ggaatgcggc gatgctgagg
     5461 gaggaggaaa gagacttctg ctgcgcgagg acgctgaagc cctccgcccc gagcattgcg
     5521 aacaggatga cggcaaagat cttcttcatg gtcgtgcgct tccttccgcc ctacttctcg
     5581 gccggcggca cgggcgtgca catcgccgag acggcgagta cgagatcctc ccgggcgatc
     5641 gccaccgagg cgctgcccgt cttctcggcg atcacgaagc tgaacgcccg gccgttctcc
     5701 aggccctgaa ggacgacgag gcccttctca cgccggaggg actcgatcgg ggtcgtgcgg
     5761 ttctgcccgc tcgccggcgt cgtggagagg agcttggccc cgaggtcgat gatgacgaac
     5821 tgcggaacga gcgccccgtc gggagagtcg tccatgcagc cgtcgatgtc gcagcggccg
     5881 acggtgacga ccgagcacag gaagcggttc gttccggtca gattgtcggc gacggcgtcg
     5941 ggcgcgccgg cgacgaggag cgcggcaacg agccagcaga acaggatcgg tttcttcgac
     6001 atcttcattc ctccgttcga tgagtggtcg aactctcgca ttctcacgag tcgcgaagcg
     6061 cctccgcctg cagcgcctgg tagcgctcga tcgcgacgcg cgcgttgaag agcatgcgct
     6121 cgcggccgag ccgctcctcc agccccgcct tgcgcacgac ctcgagggcg cccgggttca
     6181 ggccggccag ccagaagatg ccgccgctct ccgtcatgcg ctgctcgcct tccatgagca
     6241 tgtgcagcgc cgagtactcg atgtcgggca cccggctcat gtccagcgcg aggacccgcg
     6301 gccggtgctt cgccacgagc gccttgatct gctcgcccac gttctgggcg ttggcgaagt
     6361 agaggcgccc ctcgggacgc aggatcaaaa ggccctcgaa cgtttcgtcg tcggggtgct
     6421 cgggcgacat cggccggagg acgtcggcgc cgcgcttgcg gacgatcacc gaaacgcgcg
     6481 ggtgggcggt ctgtcccgcg aggccgatca tcgagaggat gaccgcgacg acgatgcctt
     6541 tcagcgtgcc gaacacgagc acgccgagca tcgccgcgat cgcccaccgg aactccatcg
     6601 tccgcacccg gcggatcgcc gcgaattcca cgggctggat cagcccgacc gagtagacga
     6661 tgacgaccgc cgcgagcgtg gcgttcggca ggaggcccag caagggcgcc aggagcagca
     6721 tggtcgcgag cgccgtgccc gccgtgacga gcgaggcctt ctgcgtccgc ccgcccgccg
     6781 cgcgcacgac cgcggtctgg gacgtgccgc cgcctgccgg catcgcgccg aggaacgcgc
     6841 ccccgaggtt ggcgactccg gtcgcgacca gctcgcggtt cgcgtcgatc ggaggatcct
     6901 cccgccccgt gaaggcacgg cccgccgcga tcgtctcggt gaagctcatg agcgcgatcc
     6961 cgagcgcgcc cggggcgagg tccgcgagca gcgcgggatc cggcagcgtg agcgagggga
     7021 agccccgcgg gatcaggccg actgtcgaga cgccgagcgc cttgaggccc aagaaccacg
     7081 atgcggcgat cccgccgccg accgcgacga gcggcgcggg ggaatggggc cagaggcgct
     7141 ccatgccgac cagaacgagc aaggtggccc cggccacggc gagcgtgacg agagacgtct
     7201 cgggcacctg gcgcgcgacc ccgacgacgt cgcggaagaa gccctgcttg gtgacgtgga
     7261 cgcccaggag cttcggaacc tggtccagga ggatcacgag gccgatgccc gccttgaagc
     7321 ccgtgagcac cggcgtggag atgaagttgg cgacgaaccc gagcttcagg agcctcgcga
     7381 ggagcagcat cgcgcccacg agcgcggcca gcgtggcggt cgccgggacg agcctcgccg
     7441 gatccccgtc cggcaccgcc aggcccagct gagtgccggc caggatccca agcgtcgtcg
     7501 tgctgctgac gctgagggcg cgggaggacc cgaggagcgc gtagacggcc atcgggacga
     7561 aggcggtata gagcccgacg gcgacctgca gccccgcgac cgttgcgtac gccatggcct
     7621 tcgggaggac gaccgccgcc gccgtgaggc cggcgaccag gtcgaagcga agcgtgccgc
     7681 cgtccgcccg gtctgtcgtc atgctccctc cgatcaattc agccggatgc cgagcctgaa
     7741 gctgaggatc tccttcgccg cgaacggaga cgagtcccac gcgagtgtcg ggccctcgac
     7801 gaggatgtcg atgattccgg cgttcagcat cacgccgagg cgcgtgccgg cctggaagta
     7861 gtacgtactg cccggctttc cgtggacgcc cggcgacgtg tcgacctctt cgtccccgcg
     7921 caccttgggt ccgtactcga cacgcacggg cgtcagcgag agcccgagga ccttgagcgc
     7981 ataccagcgg atgctggcgg cgagagtacc cgtgaggtac ttcgactcgc cggacgtcac
     8041 gcggttggcg acggcgaccg acaaggccgg gacccactcg aaccggccgc cccagcggtt
     8101 gcggtcccat gcgacctcgc cgccgaggag gccgagcccg tagtggcgcc cctcgaccgc
     8161 gtttgcgggc gtctccgcga ccgtgacgcc ggagaccacg taccaggagc ccctgcggcc
     8221 ttcgatgtgc ccgtagggcg cgggcggcgg cacccacgcg ccgtcctgcc ggacggccag
     8281 cgtctccttc gacaggagga gcccgcggtc cttcgagagc ttgctggacg gcggagcgta
     8341 gatgagcccg acgccgaaca tccattcgac ccgcggcttc agccagttga cctccatcgg
     8401 cgcgtcgatc gtgaggaaca ggccgtcctt cagcttgtag ctggcgcgga agaagcgcgt
     8461 gacgagctcg gggccggccc ggtcgcctcc gtacgtgacg cgcagggctg caggcgtgaa
     8521 gcccagtgtc gcgcggaggc cgaccggcag cacgaggtcg accccgagcc cgacgaggcc
     8581 ggcgcctccg cccccgtccg tgtactgcct gtagccgacg ctcgcgttca gcgcgtaaag
     8641 gaacggattc aggtcctccg agtacttgat cagcgtgccg cgcgcggcga tgtcgacgaa
     8701 atcgcgcgtc gggttccaga tcgtctcgag cccgagcaag gcgtaacgcc cgggcggcgg
     8761 atccggcttc tcgcgctcgg cacactcggc gccctcgcag ggatgggcgg gcgtgaacca
     8821 cttcgccttg tagccgcgcc acgcgtcgga gcgctccggc gccgtgtcgg tccccttcgg
     8881 cgccgcgagc tgcgcgagac gcagctcccg cgcgaggacg aggagctcgg tgagcgcgcg
     8941 ccgcgcgcga tctccctccg gtgagaggca ctcgtacggc acgttgtacg gttggtccgt
     9001 ccgcttctcg caccgggcgt cgccgccctc gacgacgtac gtcttgtccc ggtggtcgtc
     9061 ccacttgtga tacgaggcgt ccgggatgcg cgccgcccgc tccgtgggca gcttcacgag
     9121 cttctcgatc ggcttccaga ccctcaggta gccgatgcgc ccgtcctccg cccgctcggt
     9181 gtgcgagccg gagaaggagt cgcccacggc gtgcatgaac tcgtagaggc cgtgcgagct
     9241 ccggcgcgtc gcggggcctc cgatgacgac gacttgccgg aggaggccgt cgagaaggtc
     9301 gttgcagcgg acgaccgcgc tcgtcgcgag cgcgcgcggc acggcggtgc cgggcagcac
     9361 gtccgattgg gcgtcgtcca gggtcgccat gtagtgaaag cactggccca cgtcgctgaa
     9421 ctggcgccgg aaggccgcgt ccggccggtc ggtcaggggc tgcggccagg cgaggagagg
     9481 attcgacgcg ggcgcgtcga cgcattccct cgggggcaca tcgccgcgcc cgaagcagaa
     9541 cggcaggtcg agcgcgccgt cgttgatgag gtcgcggagg acctcgggcc ggggcgcctg
     9601 gccgtcgtgc ccctcgacga gcgtgcggta cgcgagcgcc tcgacgacga cgtggccctt
     9661 gctgtcgaac gcggacgcgg gcggcgccgc aatcagcggc gccgcgagcc agacgagggc
     9721 gatgcgcctc gcgacgacca cggtgtcccg gcgctcaggt tcccgcggcg gaccgcgtca
     9781 gaacgcgagc gacacgtaga cgatcgggcc cgagtacgtg tactcgaagc ggatggtcgg
     9841 gtcctgttcg cgctcgaacg tgatcttcgt gtagctgtat cccccgccga agccgaagtt
     9901 cttccacggg taccagtcga gatacgcgcc ggcgtcgacg aagctgccgc tgtagcccga
     9961 aatcgtcacg gtcggcagtc cgcgcgcctg ggcggagatc atgaggcccg ggaggagcgt
    10021 gaaccggccg taggcgccga tcgcgggaac gggggccacg acactccggc tgtcgctcgc
    10081 ccggctcccg gagaccgtgc cgccggggcc ggtgatggac ccggatccgt tgaactcggt
    10141 cgagttgatg agcgccgaga acccgagttt cagcccgagc tcggcctcgc ccgtgttcac
    10201 gaacgagtac gcgtagtagg cgtcgacctg cgtcgccctc aggttcgagt cgatcgtcgc
    10261 cccggcacgg tagacgtggt cgccgaactg gatgtccttc gcgatcgtgt gcgtggcgga
    10321 ccggttccac gtgatgacgc cgaagtcgaa gcggccgtgc cgcccgaagc gccagtaccc
    10381 gctcgcgcgg aagctcgtct tgttcgcgtc ctggccgagg tcgtcttcga agttgatttc
    10441 ggttccccgg cccaaagccg ccgagtccaa gcgcagggtc gtttcgaagt tctggaagaa
    10501 gccgccgagg tcgaggcgga agcgctcgtc catccccttg acggccgcat acgactgcgc
    10561 ggcggccgga gccgcgaggg caagcatggc ggccgcggcc aggatgcctg cggagcggaa
    10621 gagtttcatg tgtcctccct agtccttaca gtttatgtcc caaacggaaa aaaggccgcc
    10681 cccgaagcga gggcggcccc aaaaaaacac ggctgaatcc cgattcagtt cgcgtcgacg
    10741 ggcttgatct cgccgaccgc ggcgacgatc tcgacgcggc ggttcttggc gcggccctcg
    10801 gcggtcgcgt tgtcggcgac cgggctggcc gggccgaggc cctggaaggc gagccgtgtg
    10861 tccgcgacgc cctgcgcctt caggaagtcg taaacggcct tggcgcgggc ctcggagagc
    10921 ttctggttcg tcgcggcgct gcccgtgttg tccgtgtagc cctcgacgcg catgttgacg
    10981 gccgggaaga cctgcgcgat gcccgcgagc ttcgcgagcg tgagctgggc cgacggcttc
    11041 agcgcggcct tgcccgtctc gaacgagatc ccggggaggc tcatgacggc gccgcgggcg
    11101 ctgtcgcgcg tctccatgat ggacgacatc gaattcttga ggcggccctc cagctcggcc
    11161 ttctccttct ggagctgggc ctggtcggcg agaagcctgg ccttgtcggt ttcgagcttg
    11221 gcctgcgcct ccttcagctg ggcgaccgtc atctggctct cggcgagggc ggccttcttg
    11281 gcggcctcgg cgtcggcctc gagcttgcgg ccgtagtcgc gcagcgcctc cgaggacaga
    11341 aagaccgcgc ggctggagta gtcgttgatg accttcttgc tgccgccggc cgccgagttc
    11401 tgggcctgca cgagggcgcg cttggcctcc tccgccgcct tggggttgag cgagaacgcg
    11461 ccggtgcggt ccgcgagcgc cacggcccgc tcggcctgga cgatcgcgac gggcttcttg
    11521 tccttgtacg tgaggccggc gatcgtgttc gtgtcggcct tcacgatgcc cgtgcggaag
    11581 ttgctgaagg cgaacgtgga gccgggggcc ttcttcgggt cggccgggcc ggacgtcgcg
    11641 atgacgtatt cgctcggcct gccgacgagg gagaacggct cggccgtcac gatgaggccg
    11701 aacgacttct tgcccgtctg gtactggccg gagccggacg cgttgctctc attgacgatg
    11761 agctcgccga ggttctcgac ggcgccgtcg cgcgtgatcg cccagacgac gtacgaggag
    11821 acgtcgccgc cgaacaggat cgcgggctcc atcttcttga aggagacttc gaccttcgcc
    11881 tggccttcct tgtaccggac ggccgcgtcg agcctggccg cgttcggcgt cgcgttcgtc
    11941 cgcgccatcg cgaggtcgat cgtggtgccg tcggggaacg tgacggcatt gaggttgaac
    12001 tccgtcgcga aggcggagcc ggccgcgagc agcgcgacga cggccgcgca cgcgagcacg
    12061 cgagggaggc tgaatcggga cttcatggtt ttctcctggt tgtcctgacg gttgactttc
    12121 gcgggaaaag cccgcggctg gaggcgaggc gcctcgtttc catcctacta aacagctatt
    12181 tcttcttcga cgggaacgtc ttgaacagct tcttcatcgc gtcgtcgacc agctcctgcc
    12241 gcttctcggc cgaggcctcg gagtcgagcg ggcttctcgc ggcgccgcgc cagacgagct
    12301 tcttcgtcga ggcgtcgacg aggtcgacca tcaccgtgcc gatcgggatc gacgccccga
    12361 gggccgagcc ggtgccgatg tccccgaacg gaggagccca cgtgccccag ccgtattccc
    12421 atccggggtc gtacgaattg gtcgggtatt ccttcgagag actggggtgc accgccacct
    12481 tgaggtcgcc gccgccgtcc gtgcgcacga accctttgcg ctggagctgc gcgtccaccg
    12541 cggacatgat ccgtttcttg acggcctcgc tgcggacttc cttcatctcg gtccacgaat
    12601 aggacttgta acgggagaag tccgcagccg ggtcggcctt ggattcgacg gtgatggaag
    12661 aggcatggcc gacggcgctc acgacggccg cggccagtac gagcgtgccg aacggcgtcc
    12721 tcatcggcat gcctccttac ttgccgaagc ggaaggtcac gccgcccgtg atctcgccgt
    12781 tgctgtacca gctcgtcgag taggcgtagc agtagtagta gtcacaccac acgccctggg
    12841 acgtcgtgtt cccgaggttc gtgctccgga agcgtccctc gacgcggatc gccgcgctcg
    12901 gcgtgatgct ccacttgccg ccgagcccga ggctccacgt gaagttcgag ctggaggacg
    12961 tcgcggcgcc gtcgacggac gggttcatcg acatgaggcc gccgccgaag aggaagtagc
    13021 cgctggcgcg cggcgagccc cagtgccaga gcgcgtcgat ctcgaacgag tcctgcgcga
    13081 gatggccgat cttgccggag ccgccggaga tgcccttgta gttcgtcgca ttgaggttcg
    13141 cggacgtgtg gccgtacccg aactccaccc cgaacgcggg cgtcacgttg taggccaggc
    13201 ggccgccgaa ggtgccggcg ttgtcgacgt cgaccgtcgt cgagccgccc tggaagatct
    13261 gctggccgaa catgacgccg ccgtagggcg tcagctccca cgtgccgggg acgttctgcg
    13321 ccgaggcggg aatcgccgcg gccgcgagca cgagaagcgc ggcgaaggtc aggcgatgga
    13381 acgagaactt catgtcaacc tccaagtcat gtgtggcgag cgccgccgca gtccgcggtg
    13441 cacgctcagg gagtcgtgtc gaggagaacc ttgcgccccg cgaggggctg aacgggacgg
    13501 ttcgtcgtgt tcatgccggg cacggtgacg aacgcgccga gctgctcgcc cgtgatgccc
    13561 atcggagact ggagcacgta ccaccggatc ccttccgtgc acggcggagt cgtgagcgag
    13621 ccgtcgtacg cgagataggc ccggttctcg ggaaggagct tcgcgaggtc gaccgtcacc
    13681 gacgaatcgt ccttccgcgc gcctttcacc ttcgggagat ccgagagcag cggcgtgaac
    13741 gccgggttgt ctccgccctg gacgaggaac acgccgacga cgaggccggg cttgccgtcc
    13801 ggccccgcgt ggacgaggtg catctccatc gggtagtgga ggccgtcgag cgagtgctcc
    13861 gagggcgcgt ggaagtggag ctggagaagc gcccaggccg cgccgtcgat ctcgaccgtg
    13921 ctgcccttct cgacgtcggc ctgcacggtg tggcccttgt tcacgaggtg cagcttcgtc
    13981 gtcccgtacc tgaagacgag gttcgggagg tccttcgcct cgaccgggaa cggcgcccgc
    14041 gtcgcgaggt cgacgggaga ctgctttttc cccgccgcgc acgcggcgtt gcccggcagg
    14101 ctcacccagt cggagggacc ggccgtttcg gcgttcgcca cgtagcccca gttcctctcc
    14161 tggacggccg gcggcgccgg cgtgacggac gtcgtcggcg cgttcgagct gcaggccgaa
    14221 aagaacaggg ccgacgcgag taccggaaac gccccgagcg agacacgaga attcatccac
    14281 cctcccttgg gcgttcgttg agatcttagc gcccgccagc ctctcctttc atgatgcaag
    14341 tacatcaagg gattggtttt gcgggcggat cgaagacggt gacgggcagc gtcaccgtcc
    14401 gctccaggat gccgacgagc cccttcccca cgtcccgccc gggcgtgagg gcgaccttcg
    14461 gctcggccat gctccccgtg accttgaccg ccacggcaag gaagttgcgc ccgaggatcg
    14521 tgttcaccac cgggaccgcc tggacgacct tgtcgagcgt cgacaggggg tgggacagca
    14581 ccatcaggtc gagtgaccgg tccaggtaac cgactgttcc ggtcgcggcc atcgtgatcg
    14641 aaggcgactt gagcgccgcc tcacggatcg aggcacggcc gtcgccgagg tctccgttca
    14701 gggtgatctc gtcgtatggg atcgcatccc ccacgcggcc ccccgacttg ccggcgaaga
    14761 cgtccgtcgc gttgaggacg ccgaggatcc gcgtcagcag cgtggccttc ccgatccggc
    14821 ccttcgcagt cttgaacctc agcggacccc gcagggcgcg cggcagctcc gaagccggcc
    14881 cctctccccg gacctgcacg ctcgcctcgt agaccccggt catccgggtg ttctcgaaac
    14941 cgaaacaggt cagcgggacg ttgatgtcgg ggcccgcggc gtccgccctc acgtcgaccg
    15001 ccatggcccc accgggaagg aagcgcgctt ccccggtcgc cgagatgccg cagatctcgg
    15061 ccttccgaag gcgggccgcg agggagtccg gcccgaattt caggtccgcg aggacaggct
    15121 tccagacgag atcctcgtac gaaaacgagt cgagggaaac acgaaggtcg ccacggatcg
    15181 cgagcggcgc gcgcgcggcg gtctcccggg ccgcctctcc gctttccttt ttcttcccct
    15241 gcgcgacccg gtcgaggatc ttctcgattc gcgaccacgc gaggtcgccc gttccgacgt
    15301 ccatgtcgag agcgatggcc ccgtccccga gcgtcgcgct gcccgccgcc gagaaccgct
    15361 gctcgtcgag gacgaacgag gacgaggcca cgtcgaaccg gttcttcgcc gcgcgcagat
    15421 ccgcgcgctc gatcgtcacg ggccccgcgg gcgtgggcac ttcgaaaccc gtcgccgtga
    15481 gcgtgccttc cgccgtcgac cggccgaggt tctccgaggc aacgagcgca cggaaatcgc
    15541 cctcgatgcc gccggggtgg cgccgcccct cggcgaagag cttctcgacg gtcgagaggg
    15601 cgaggcgccc cttgaatcgg acgtcgaacg ccgcgtcggg ccggaggaga gccatggaag
    15661 ccgtggcgtc gccgtcggct accgtgaggt tgcggatgtc gaccttcttc ccgtctcccg
    15721 caacgtcgag cgccagacgg ggcccgcccg cgacgacgag gtcgccggac gcggagaacg
    15781 cccctccccg cgcgagggcg acgcgaaccc tgttgagggc aatcggcgcc gccggcagga
    15841 gttccgccgg gagcgacgca cgcccccatc cccatcgcac cgcgtcgggc ccgatcgtgc
    15901 cgttcgcggc ggcctccagc gaccgctcgc cgccacgcca gcccttccac accccggagg
    15961 cgaggaggga cgcgtccagc gtccgaatgg cggcgtcgga aacgcggacg gcgtcgtcgc
    16021 cgacggcgaa gcgtcccgaa gggatcgaga gggtcggaag agaaggggaa gaaacgagaa
    16081 cttctttgaa ggtgccagag gcgtcgaccg aggcctcgtc gagacgagcg agcggcccgc
    16141 tgacccgccg gacgtccact cggaccgtac ccgaaaggtg cgcaacgctc ttcgcgagag
    16201 catccgccgg cggtttcttc cgaacacctt ctaagaaatc ttctaacgac acctcgatcg
    16261 agcccaaagc gttctcgagt tgcgcggacc ttcccaatcg cacgcgcgcc gcgaggccgg
    16321 agaacgtcga atggccgagg ctccctgaga gtctgctcgc gccgacgcgc ttgccgtcga
    16381 aagcgaattc gccgcggtcg acccgcagcg gccacgggat ccgcctgtag ttcgccgaga
    16441 accgaaggtc cgatacggac acgccggtat tcaggtcgcc ggcgcattct ccgatcgcga
    16501 tgcggcccgt cgcgcgcccc tcgaggccct ccaccagcgc gagctcctcg cggaaggacc
    16561 tgtcggggat cgcgcgcccg aggatcgcgg caacctcggc gagatccgcc ttcacctgcg
    16621 cctccacgcg caggcggccg tccccgggcg cgaggccgac gaggaccgtc ccgccttcgg
    16681 ctttcgactt cccgaggcgc gcggaggcgc gttcgacggt gaggacgccc ccctcgagcg
    16741 cgacatccgc ggagacgtcg cggaggtcga ggtccgggcc ctcgacgaag acccttgcgt
    16801 cctccacctt cgcccggatc ctcatcctct cgagcgcgaa gaggtccgag ggcgaggaag
    16861 cgaccgagcc gaaggcgatc gacgtgaggc ttccgccgcg aaagatcgcg aagatctgcg
    16921 cgatcgtccg gtcgtcgccc gcgaacgcga ggagaagctc acgaaccgcg ccgccgtcga
    16981 ggctgcctct cacgctcacg tcggcgcgcg gtgtgaccgg gtcccagcgg aagtttcccg
    17041 aggcggtcag cgcgggagag tccaccgaga ggcgggagag aacgatgtcg gttcctcctt
    17101 cgaaggaacg gaacgatccc tcgaccttca cgccttcgac ccagagtccg cggccgtccc
    17161 ggcgcgagag gaacacgtcg gcaccctcca cgaggatccg tgaggggacg aagcgtccgc
    17221 ggaggagcgg cagaaacgcg aaggcgacgc gtacggtgcg gaccctgccg tggaccgttc
    17281 gcgggacgga cacggtcgcc gaccggagga cggcttccgt gcgcgggaac cacgcgaggt
    17341 ccatccgctc gtacctgacg gcgccggcga gggccgacga cgcggcgctc tcgatccggg
    17401 cgcggaccgg cccgaggtcg atccaccacg gcgcgagcgc gacgcccgcg gcagcgagga
    17461 ggacgaccgc gaagacggaa gctgcgaccg ggtggcgtcg aacggcggac ttcatggaac
    17521 gggcgaggcc agagtgccac agggccgcgc ccgtcgtcgg atcacgggtt cagcgcggcg
    17581 tcgacgcgag cggattggcc ttgagcgcgc ttgcgatgtc ggcgatcgcc ttctggccgc
    17641 ggacgctgtt gccggcgtcg tcgagaggcg gcgagatcac cgcgatcccg aacttgccgg
    17701 gtgccacggc gatgaggccg ccgcccacgc cgctcttgcc gggaaggccc gtgcggtaga
    17761 accacttccc cgagtcgtcg tagaggcccg ccgtcgccat gacggcgagt gtccccggca
    17821 cgttctcggt cttcatgacc tgcttcttcg tgagcgggtt ccttccgccg ttcgcgagcg
    17881 tcgccgccat cacggcgagg tccttcgcgt tcacgttcac ggagcattgc tcggtgtaga
    17941 tgtccgtcgc ccgcagcggg tccttcttga tgtactcgta ggcgtacatg aggtagccga
    18001 tcgcctggtt gcgctggttc gtgtcggact cggacttgaa gacgtcctgg ttcaccgtaa
    18061 gcgggcgccc ggcgaagtcg gagtacgtcg cgaggaggcc gttccagatt tcggcgcggt
    18121 ccttgccctt gagcatgctc gtcgccgtga tcgcgccggg gttcacgagg gggttgatct
    18181 ccggcccgcc aagggccttc ttcgcgaact cgaccgagac gatcgagttg aaccgcatgc
    18241 cggtcgcgtc gacgccgatc gtcttgacga tcgcatcgaa gcccgactcc tcggcgacct
    18301 tcgccatcgt gaagaccttc gagatcgact ggatcgagac ctcggacttg aggtcgcccg
    18361 ccgtgtagat cttgccgtcc gtcgtcacga gggcgatgcc gtagatcttc gggtcgacct
    18421 tcgcgagcgc cgggatgtag tcggcgttct tgccttcctt gatgtccttg tacttttcgt
    18481 aggccgcgtt gacggccttc tggatgtcgt cgggcgtctg cgcggacgcg gcgggcgcga
    18541 ggctcgcggc gagcgccagg gcggccgccg ccgcgagggc cttcttcacg acactcacga
    18601 cttgcctccc agcttccatc cgaagttgta cttgaacgag aactggatcc tgtaatcgtc
    18661 gaccgagaag ccgtcggaga agttcttccg cttgccccac tggaactcgc cgccggccat
    18721 gacgccggaa gccgggtaat acagcaggtt cgcgagcgcg tagtggccct gctggaagtc
    18781 gctcgccttc tggccgtccg tgttgtcgat gttgaggtac gagtacccga tcgccgtcga
    18841 gaacttcttg ttccagttgt ggtcgaggaa cgccacgatc ccgaggacgg ggagcgcctt
    18901 gccctcgatc ggcttcgtcg gattgccgaa gttgttcttc acgccgatgt cgatcggcgc
    18961 gtcgttcatg tagttctgaa tgccttcgcc gtagaccacc gatgaacgga tcacgtcgtt
    19021 ttccgtcacg ttgatgatcc cggagaggtt gaggccccat cccgtggcgc tccccgagag
    19081 gtcgaccgcg tcgacgttca ggtcgtccca cttgatgaag cgcagcatgc cggcgagctg
    19141 gacgtggccc cacttgccgg acttcttgta gtgcgccgag atgtcggggt actgcgtccg
    19201 gatcttgacg ttctggagct cgacgcggtc ggcgtagttg ccgccgtcgg cgctcgctcc
    19261 cggccgctcg agcgcgagga agacctcgtt ctcgccctgc atgggcatcc agcgcacctg
    19321 gacgttgcgg aacaggacca tgccggacgg gccccagtac tcgagctggt tcgggaagac
    19381 gtccgggtcc atgaacgggc tccacgtctg gcccgccccg acctgcccga gctcgcccca
    19441 cgcatgccgc agccggatcg tcgtctggcc tgcgtccacg ccgacgccga acatttcgaa
    19501 ctcgaagatc gtcttgatct cgccgacgga cgtcgggacc cagcctttca cgccgaagcg
    19561 gctctggcgc acgctcgcgt acgtcttccc gtcctcgccg aactggttct cataggacgg
    19621 cagcttcgtc accctgagga cgtcgaacca gtcggggttc acctgcttga agtcgtagcc
    19681 catgtcgagc tgcgcgaagc cgtagatctc catccgcggc ccggcgggct tgtcggcgtc
    19741 cgtctgcgcg cgtgcggtca gcgcgccgag cgcgagcgcg gcgatcgcga tcgttcggag
    19801 cgaggttcct ctcatcaggt ttcctcctgc gggactgcgc ccgcgattca cttgacgttt
    19861 gccctttccc acacacgcac gcgaacgcgc gccgcgtcga agagacgttc gtaattgccc
    19921 ttgaggacct tctcgcgcgc ctccggcgtc agggcggccc agagcggcgt gtacgcctcc
    19981 caggtcttga aatagccctc gggagtcgtc ggcgacaacg cgtcggtgcc gaagaggaac
    20041 ctgtccgggt ggcggttcaa cgcgcccgcc gtgttctgca gggcctccgg cgtcgcgacg
    20101 gcgtacttgg caacctcgtc ccacgagatg tcgaagctca cgtgggcaag agtcgggtcg
    20161 tcgagaatcg ccgtgacgaa cgcgacgtac tgcttgagcg ggtggacgat ccggccgagg
    20221 ccgaggtgcg cccagatgat cgtcgttttc gggtggcgcc ggaagagcgc cttgagctgc
    20281 tcgaggtaga cgggcggcgt gccgtccctg gcgaacggca tgtcgaggtc gttgtgaagg
    20341 atcacgagaa ggccggtctc cgcgccgaag tcgaggatcc ggtcgagcgc ggggttcgtg
    20401 aggctcgcgg tctctcccgg gatcttcgag gacacgaact ccttgtggat cgtgaactcg
    20461 ccgatcccga cgaagacgcc cgggaacgtc gcgagaacgc gccggatgtg gtccgccgcg
    20521 tacatgtcgg ccgggttgaa ccccgtgatc atcgggtcga accgcgcctg ctgctccttc
    20581 gtgagggagc ggaacgccat cgcgatgaag gcgtccgtga acgagtagta gtagagcggc
    20641 gcgtccgtgt gaaggtagta cgtcggggcg aagtcgcccg agttgccgta cgaccacgtc
    20701 tgctggagcg ggatcccgaa gagcgtcgag cggccgacct tcgttcccat gatcttcagg
    20761 aagtcgcgga cgtccggccc ttcctggacg tagttcgtga ggtggaagtg ggcgtcgtgg
    20821 aagagcggcg cctccaccgc ccgaagagaa gatgctccag acgcgaacgc gagggagaac
    20881 gcgagccgca ccatcaatcg caacggcgac ccgccaattt tcacaggaat tccgcccatc
    20941 tcccgtcacc ctgcggcacg actcgccccg ccgtcgagca acagaccgat gaacggagac
    21001 acacccgtca gctccgctcc cgacccgcgc agcgagcgca ggagtgcgat gccgagttcg
    21061 tccgcctgga gcagcgccgc gaggtcgatg cggagcgggc ggagtgaggc ggcgcagacc
    21121 cgttccagct cttcgacgcc cgcgccgcca agacggcccg cgacgcgcac gaccgtgagg
    21181 tctccaaggc gttcctccgc gatccgcagc atcggattcg gcttcgtccg ttgcgaagcg
    21241 cgtgcccgcg gaggccaccc cggcaggcga cggcattgcc ctttgtttgc agtcgcttgg
    21301 ctgcgctcga agcaccgcgg atcggcgcgc ctcgggaagc cggacgggat cccgccagtc
    21361 gtggcgaaat gccgtcagcg cggggtcggc atcatcggaa cgtcgcccgg cttggagacc
    21421 gttccgcccg cgggcgcgta ggcgtcggac gcacccggct ggccctgata ggtccaattg
    21481 ccgggccctg ccgggttgcc cgccggcgcc gccgagccgg acgccggtgc cgtgttcacg
    21541 ggggcgttcc ctcccttgaa accgggcgtc atggactcgc cctcgaggac gatgaggccc
    21601 agcgcgagct gctcggcctt gaacgcctcg gcgccggtca ggcgcgtgat cttcacgacg
    21661 tccgtctccg gcacgctcac gagcgtgttg ttcttccagg tgtaggcgac ccagttcttc
    21721 cctttcttca ccggctcgtt ccgggcgagg tgcatgccgg ccgccccggc gagctccacc
    21781 cgccacacga cttccttgga gtagtcggtc gaccaccgcg cgaccgactt caccttcacc
    21841 ttcgggagtc gcatctccgt ccgttcgggc agggcgcgga agacgtaggt gtcgccgtcg
    21901 agcacgggct cgttcatcgc gaacatggac ccgttcggga cgaggtcgat ccggtagacg
    21961 atgatccgcg gttccttggc aggcgcagcg gtgggcgccg acgcggcggg ctgctggccc
    22021 tccagcgccg gggcgaagga gacgaagacc gcgagcgaag cgacgcgcaa cttcatgttc
    22081 gttctcctcg agtcaatgta gcaccgtgcg ggaggcctca ccacgagctt ccgaagacca
    22141 cgtagagggc ccattgctcc ggccccttcg cgacgtcgag cccggccagg atccccagct
    22201 tgcgggcgac gaggtagcgg aagccggctc cgcccgcatg gacggattcg tccacgagga
    22261 cgcgcgccga gcccgcgtcc gtccagccgg cgccggcgaa cacggcgagc caccacctct
    22321 tcgtgacggc ccaccgcgcc tcgccgtcga gcgacacggc cgtctcgccc tggtaacgca
    22381 tcgcggggat gcctcgcagc ctcacgaacg gacgtgcgta gaagggcgca tcgcccgcga
    22441 cggcctgcgt cctcagccgc gtcgaaagaa cgagccgcgg gtggacgtcc cagaagaaga
    22501 ggccgtcccc cgtgtaccgc tggtagtcgt ggtcgccgcc gaggtacggg ccgtagagag
    22561 ttccggacgc cttgaactgc acgccccggt tcggcgtgaa cgtgttgtcc cgcgagtcga
    22621 actcggcgac ggcgccgagg ccggagtccc ggttcccgaa ctcgggcgcc ggaacgtccg
    22681 gcggagcgag tcccgagtcg aagcgcgtcg tcgtgtccat gaaaatgtag cgcgcgccca
    22741 ggaagaggtt cgagcgtccg acgcgccgct tcagctcctg gaagacgacc cagccgtcga
    22801 ggttgaagcc gtaggcgcgg ccctgcgcgt cgtacgtgtc gagctgcggc gacatgcggc
    22861 cgacgacgcc cgtgtaccgc caccggtcgc caccccagta cccgacgtgc gccacggccg
    22921 ccatccacgt cccgttctcc gtcgcggcgc cggcgagcgc cgacacgtcg ggcgggaccg
    22981 gcttgcccgt gacgccgagc ggcgcgtctt tcgtcccggc aagtcccccg ccgtggatct
    23041 tcaagagccc gagcgccaga ccgtagccca ccgcgggctc ggtgatcggg acgggaatcg
    23101 gcaggatgcc ggtcctcgtc gcggcccagg ggccgacgtc gaaggctccg tcctcggggt
    23161 ccttgaacag cgagggcttc gcggccggcg cggaatcgtc cccggggggt gcctgcgcga
    23221 acaccgcggg cgcgagggcc aggaccgccg cgacgacgag cgcgacgacg gccccgcgcc
    23281 gcatcagaag tattgcctcc agtcgaaggc gaggcggaag tagaccgacg tcgccgtctt
    23341 caggtccggc gccggcgcgc cgaccggcgc catgaccgtg accctagtcg gcttgtcgaa
    23401 ggcggcgccg atctggatga ggaccgccgc ggcctgcgtg gtggcgtagc tgcggaaggg
    23461 acgcacctcc acgatcggga cgtcccagcg gatcgaccgg agcgagaccg ccgtcagcgc
    23521 ggtgcccgac ccgggcgcgg agacgaagag ctggtcctcg ccgcccgaat acccgaagaa
    23581 ggtggcgccg acctcccgcc cgaggacgaa ctgcacccgc ccgaggaacg tcgaaaggcc
    23641 cgtctgccac gggatcacgc cgccgctcgc ggcgaccatc gccatgcttt cgtacgtctt
    23701 cggcgcgagg aacccgagga tcggcgcggc gaggatgagg tcgcccggga tcagccagaa
    23761 cggcatccgg atgcgcgtcg cgatcccttt gcgcgcggga gtccgcagcg cgaggttcgc
    23821 aacgaggccc gaggtggagc acggatccgc gcagacgtcc ttggacccga tctcctgcag
    23881 gactccggcc tgcaggaaaa tctggccgtc gccggcctcg ccgagaacgt cctccaggcc
    23941 caggcctgcc cgcgccccga tctccatcgt gccgttcgcg cggttggtgg attcggtcga
    24001 ctcgaatccg ccgtccgccc atccggccga gatcccggcc gcgaggccga tgaacggccc
    24061 gatttccgac cggaagcgcg ggagcgctcc ctcgggcggc ttgcgcccgg gcttcggcgt
    24121 gtcgaggagg atcgacgccg cctccttgac gaccggtagc ggaatttcac ccgccgccgg
    24181 catcttcatg gtcttgcacg tgtcgaagcc cgggactccc ttcgcccagt cgatcgggat
    24241 cgcgctcaca tccgccgcga gcgcccagcc cggagaggcc gcgtcgagga cctggttgaa
    24301 gctctcgcga accgccgccg aggcgcgcgc gaggtccgcc ggcttcatgt tcgcgtcgcc
    24361 gaacatcagg atcggcttcc cgttccacgt gacggcgtcg aacccgttgc ggttgtagta
    24421 gtcgtgcgtc cccttgcggg tcgcgacgtc tccccacgtt cccgcgacgt gcccggacgc
    24481 gaacgcgtcc tcgaggaagt ggacgccgaa cgcctcggcg gcgagcgcgg tgagggccac
    24541 ctcggacctc ttgtcctcgg gcacgcggcc ggcctcgagc tcgtacacga gacgcatcgc
    24601 cgcaatgtgg taccgcgccc aggtgccgag gccgttcagc tcgacgtcct tcgtgaggct
    24661 cgcgaggagg aactccttca cgtcgtcggt gtcgcgcgtc aggaggaagt ggacgttgtt
    24721 cgagcccgcg cgcgtggcgt actcgggatc cttccgctgg agatcgaggt cggacttcgt
    24781 gaggcggttg cgcgcctcgt tcttgttctt cgccgtcgcg agccccttgt cgagcaccga
    24841 cgtgaccgca gccacgtcca ggacccactt cgtctcgaga acggtcttga gcagctgatc
    24901 ggccgaacag gaatggtctc cggcgagcgc ggggaaggcg gcgaggtcga tacacccggg
    24961 cttgttcccc tggtctccgg cccacggctt ctcgcaaaga cggtcttcgt ggcccttgcg
    25021 ggcctcgccc cagagcttct cgaggaccgc cttccgcgcg ggatcgaggc cctcgaacgc
    25081 gctgcccgcg atgttccggt gctccgggta gatccacgct cgcgccggcg gcgccgcgga
    25141 gacggcgacg aggaggaggg cggcgaacgt tcggccgcgg ctcacgatca gaacccgaag
    25201 accagcccga cgcgcggtcc ggtgtacctg tattcggcgg acagcgtctc gttgtccttc
    25261 cggtacttct tgatcgtgat cttcgtcatg tccgcgccga aaacggcgcc gaagttcttc
    25321 gtggggtacc acgcgaacga gagcccggct tccaggatgc cgccggcgat gtcggtcgag
    25381 ctgaaaccga tcgcccgggt gtagaagtcg aacgtccagt gattcgagat cgctcccgaa
    25441 actccgaggc cgacgagcgg cgccgggaga ccgatgctgt agtcggcggc gacggcgctc
    25501 ttcgtgatcg tcccgtccgg gttcgtgatc gtcgccaggc cgttcaggcc cgtctcgaag
    25561 tgctcgtagg cgatcgagag gccggcccag accttgacgt tgtccgcccg gaagatgtcg
    25621 tagtgccagc cgacgtacac gtagttgttg tcgaacttgc cgtcgacggt gacgcccgcc
    25681 ttgtacgtgt tgccgttcca gacgacgtct gcgtcgagcg cgcgcgtgcc agagcgattc
    25741 agggacgagt acccgaagtc gaggtagttc cgcttggaaa agcgccacga accatcgccc
    25801 ctgaaatccc actgggcgga ggggaccccg aagacctgct cgaggtcgag cgtggtcccg
    25861 atcgcggcga ccccgccccg ggtgacgtcc agccgggcct gcgtgtcgaa cgagttccaa
    25921 acgccggcga gctggatgac gacgggcttg tcggcggcct cgggaaactc ggagaacgcg
    25981 acgtcggctc cggcggccgc gggcgagaac gtcaggacgg caaggccgag tgcgagagcg
    26041 cacgaggtgc gtttcattct gttacccctc tctgtgacgg aagaatgcta agggcgtacc
    26101 cgttcgtcaa ggcgtcagtc ttgggcccaa gccactcctt gtgaatgagt tgaatgaatc
    26161 aagcgtcgat tcgacccgtt gatgcgagcg catcaacgcg aaatatcgcc actcgtggtg
    26221 gaatcccgac atggcgtcgg tcatgactgc ggaggcgctc gaattcgagt cgacgctcgc
    26281 ggaggtcacg gcgcggcttc tcgcggcacc cggggacggt ttcgagacgg agctcgacca
    26341 cgcgatcgag aggctcgccc tgttcttcgg cgccgaccgg gggagcatcc tgctcttcga
    26401 ggacgacgga acgacgaacg tcgtcgctgc gttcgccggc ccgactctcc tgaccgttcc
    26461 gaaggggccc acgcagctcc ttcccaactg ggccggccag gcccggcggg gtctgggctt
    26521 cacgatgcac cgacccgacg agatcccgga gtcctggacc gaggagcggg cgttcgtggc
    26581 ggcgaacggg atccagtcga acctcatgtt tccgctgcga atcggcgaga agccgatcgg
    26641 actgctctcc ctctcgtccc tgacgcgggg ccgcgactgg tcggcctcgt ttctgacgcg
    26701 cttccggctg ttcggcgaaa tcctcgcgag cgcgatcgtc cgcgcccggg ccgaacgcga
    26761 cctgcgcgcg agcctcgccg agatctccaa gctcaaggag cgcgccgacg ccgagaacgt
    26821 ggtgcttcgg gaggaggtcc gcgacgcgca gggcttcgac gagatcgtcg gccgcagccc
    26881 cgccctgatg caggtcctct acatggtcga gcaggtcgcg ccgacgggaa gcgccgtcct
    26941 cctgaccggc gagacgggca ccggaaagga gctcctcgcg cgcgcgatcc accggcgcag
    27001 ctcccgcgag gggcggccgt tcgtcgccgt caactgcgcg gctctcccgg gcacgctcat
    27061 cgagagcgag ctgttcggct acgagcgtgg cgcgttcacg ggtgcgctcc agcgccgcct
    27121 cgggcgcttc gaggtcgcga caggcggcac gatcttcctc gacgagatca gtgacatccc
    27181 ggccgacatc cagggacggc tcctgcgcgt ccttcaggaa gggacgttcg agcgcctcgg
    27241 ctcgtcgcac acgatccgga ccgacgtgcg ggtcgtcgcc gccacgaacc gcgacctcga
    27301 ggccgcggtc gccgagggaa ggtggcgcgc cgacctcttc taccgcctca aggtgttccc
    27361 gatcgaggtc ccgccgctgc gccatcgccg cgaagacatc ccgctcctcg tctggtactt
    27421 cgtgacgcgc aagaggacct ccctcgggcg ggccgtgacg cgcatcccgg acaaggccat
    27481 gtcggcattc cagcgctacg actggcccgg aaacgtccgc gagctcgaga acgtcatcga
    27541 gcgtgcgctc atcctgagcc ccggggacac gctcgtcgtc gacgagcgga cgttcgcggt
    27601 cccttcgaag acgcgcgccg aatccggcat ctggagcctg gaggacgtcg agcgcgggca
    27661 catcctcaag gtgctcggcg agtgcggctg gcggatcgcg ggccgcggga acgccgccga
    27721 gcgcctcggg atgaaccgca gtacgctgcg ctcgcgcatg gaaaagctcg gaatcgagcg
    27781 gccgcaggct tgagacgctt cgcggttctc gcggcggcat tcgtcgtgtt ctcggggagc
    27841 ctcgcgtcgg cgcaggagaa tgggaaagag aaagagattt cttcgagtga acgagcgcaa
    27901 gcggcggcgt ctgcgcaaga agctctcccg gcgcagacgg tcgcgccccc aaagacccct
    27961 tctaagaaat cttcctcctc ttcttctccc tcttcttctt ccgagatcga ggagctgaag
    28021 gcgcagatcg caaagcagga agagacgatc cgcgccctcg aggagcgtct cgcgaagctc
    28081 gaggccctca cgaatgcgag ccaggcctct gccgggctcg aggagcggct caagcagctc
    28141 gagacggagg tcaagaagct cccggaggcc gaggacgtcg tctcggtcgg cgaattcccc
    28201 ggctcgttcc gcatcccggg cacggacgcg gccctgaagc tcggcgggca ggtgcgcgtg
    28261 accgcggtcg agagcctcga cgccatcggg accgacgacc gcttcgtgac gtcctcgatc
    28321 cccgttgaag ggagcgaggc ggccggcaag ggcgcgcgca cgacgctcgc ggcgaacgcg
    28381 agccgtttca acttcgactt ccggacgccg acgggcgtcg gcgcgatgcg cgccttcatc
    28441 gaggtcgggt tcgacatcaa ttcgaatgcg gccctgcgcc acgcgttcgg ccagtgggac
    28501 cattggatcc tcggccagac ctggtccacc ttctcggatc ccgaggccga gccgttcggg
    28561 atcgacaacg agggcctgaa cgcgatctcg ctcttccgcc agccccagat ccggtacacg
    28621 acgaccttca gagacgccta cacgatcgcg gcctcgatcg agaacccggc gccggacgtc
    28681 acgaacgcct ccggagtgaa cctgatcccc gacttcgttc tccgcgcgcg atgggacccc
    28741 ggcaagggga agggctcgct gcccgtcctc ggccagctcg ggcacgtgca gctggcggtc
    28801 cttctccggc agatccgcgc cgagtcggac ctggacccga atcagaccgc gtcccggggc
    28861 ggtctcggcc tcggcctgag cggcgtcctc cggacgggct ggtgggccga gacggacgac
    28921 gtgaagttct ccctctacgg cggcaacggg atcggccggt acatcacgga tctccgcgcc
    28981 gcgggcggcc aggacgcgta cttcgacgcc gcgaccggcg agatgactgt tctcggtgtc
    29041 acggccgcct atctcggcta cgagcacgcg tggtcgaaca cgctccgctc cacgatgacc
    29101 atgggctggg tgtgggtgaa cgtcctcgag aaccagccga acgacgcgct caagtacacg
    29161 cggcgcttct cgctcaacct cgcgtggtcg ccgatcacgc ggctcgatct cgtcgcggag
    29221 ttcctcaccg gcacgcgcgt gaacaaggac ctccagagcg gaaccgccac gcagatccag
    29281 ctcggctcgc ggttccggtt ctgacgcgat gcggcgcctc atccacaaga cggcctcggt
    29341 ctggacgaag gaggagggcg ccgccctcct cctcccgctc ctcgtggcgt ccatcttcgt
    29401 cgcgccgctc ctgagggact ggttccccgt cctcgcggcc ctgggcacgg cgtgggccta
    29461 cgcctacgat ctcctcgagg tcgtgcggcc cggatcgttc caggcggcgc ggagcgtgac
    29521 ggaggggtcc tacccgcccc tcctgtacta cagcttcgtg acgctcacga ccgtcggcta
    29581 cggggacgtc acgccgcttt cgtccgccgc gcgcgccctc tccaacctcg agtcgctgat
    29641 cggggtcctc ttccccgccg tcctcatcgg gcggctgctc tcgaagcagg tcgccgggcg
    29701 cccgccggag ccgcccgcgc ccggctgact cattcggccg caggctgcgg ctcgtctccg
    29761 gcgtcgatcc acgcctcgag gagcgtgtgg ctcacggcga ggacgacggg gcccacgaag
    29821 atcccgatga gcccgaacga cacgaggccg ccgatgacgc ccgcgaagat caggaggagc
    29881 gggaggtccg cgcccttctt gatgaggatg ggcctcacga cgttgtcgag cgtgacgacg
    29941 gggatcgacc agacgaggag gaacgtcccc catcccgtct gcccgctgcc gtagagccag
    30001 atgatcgccg ggagaagaac gaggatcggg ccgacctgcg cgatgcacag gacgagcgcg
    30061 acgacggtga ggacgccggc gaacgggacg cccgcgatcg cgaggccgac ccctgcgagc
    30121 accgactgcc ccactgccgt cacgacgacg ccgagcgcga ccccccggat cgcctgaccc
    30181 gcgagcacga cgacgttgtc tccccggtcg cccgccagcc ggcgcgcgaa gcgccgcacg
    30241 cggtcggccg cggcgtcgcc gaggctgtag aggacggcag cgacgatcac cgtcatgaga
    30301 aggtggaaga agagcccgcc gatgctcccg agagtggaca tgaaccagtt cagggcctgg
    30361 cgcgcgtacg gcgccccctt ctgcgcgagg tcgtggacgg cgccgccggc gaggtcctgc
    30421 catgcggccg ccgcctgagt gccgacgagc ggaatcccgg cgacccagga aggcggaggc
    30481 gggacctccg cggtccggaa gcggtcgatc ccggcggcga tcgcctcgcg gttcccgagg
    30541 agagtcccga gacccgcgag gaacggcacc acgacgacga ggaggagcgc gatcgtcagg
    30601 acggccacgg ccggtccgcg tcggcccccg aaccacttct cgagctggag gagcacgggc
    30661 caggtcgcga cgacgatcgt cgtcgcccag acgagcgctg cggcgaacgg ggcgacgatc
    30721 cagagcgaga gagcgaggag cccgcagatg atcaggatcg cgaagacgat ctgcgggagg
    30781 tcgggccgac ggtcgttcat gagcgttcct ttacgcgatc gcgtcgcgcg tcaatcttcc
    30841 ggcaggtggt acgcgggcag gccaaggccg tagcgcaggg agaacagtcg gagcagaagc
    30901 gtcgtcagga gccccgcgcc cgcagccagc ggccggcccg cacctgctgc cgagagcccg
    30961 acataaaccg cggcgcccgc ggcggcggcc gtcgcgtaga gctcgcggcc gtgcaggatc
    31021 atcgggacgt ccccgcagag gacgtcgcgc aacatcccgc ccgccgccgc cgtcacggct
    31081 cccatgagga cgatgacgag cttcgcgtcc gtcgcggtct cggcgatctg cgctcccgtc
    31141 acggagaaga gagcgaggcc gagggcgtcc gccgcgagga ggcggttctc cttcggcatc
    31201 ctcaggcgcg tgaagagaat cgtcgccgcc gtggccgcgg cgacgacccc gaggtactca
    31261 ggctcccgca cccagaacac cgggagccgg ccgaggagga ggtcccggag ggtgcctccg
    31321 ccgatcgccg tgacgacggc gacgacgaag accccgaaca ggtcgaggct cttgcgcccc
    31381 ccggcgagcg caccgctcac cgcgaagacg aagacgcccg cgtagtcgag agcgcggaga
    31441 aggtcctggg agacgatgac ctagcctacg ccagctcagt attcctcgaa gtacttctgg
    31501 atcttctgga cgtcgttcgt ctgcgtgagc gcgacgcgca ggaggacgcg cgccttctgc
    31561 gggttgaggt ccatcccggc gacgaacccg agcttgtcgt cgtcgagctc gacgttgcgg
    31621 cggaccattc cgaagcccac gtgagacgtg cggacgacga cgatcccgct cttcgcagcc
    31681 tcggagagcg cgttgatcat gtcggccgtc gcgttcccgt cgccgacgcc cgcgaggacg
    31741 atccccttgg cgcccgcggc gacggcggcc ttcacgaacg tcccgtcgca cccctcgtgc
    31801 gcgaagacga tgtcgacgcg cggcgggtag atcgagcgcg gcgccgtcgc gaactcgctc
    31861 gtgaccgtgt gcttgtacgt cgggacgtat ccgtagcgga ccttgccgta gtagaccttg
    31921 ccgatcgtcc cgcgcgtcgt ggagacgaac gtctggacgg ccgtcgtgtg cgtcttgtgg
    31981 acgtcgcggc ccgtgtggac ttcgtcgttc acgacggcca tgacgccgcg gccctgcgag
    32041 gccgggtcgg ccgcgaccgc gaccgcgttg tagaggttca gcgggccgtc cgcgctctgc
    32101 gaagtcgcgg ggcgcatcga gccgacgagg acgacgggct tgttgctctt gaggacgagg
    32161 ccgaggaaga acccggtctc ctcgagcgtg tcggtcccgt gcgtcacgac gatgccgtcg
    32221 acgtcgggct gggcgaggag ctcgttcgcg cgcttggcga gcttgcccca gacgacgtcg
    32281 ttcatgtcct ggctgccgat ggacgcgatc tgctcgccgt cgaggcgggc gagcttctcg
    32341 atgccggggg cgcccttgat gagggcgtcg atcgagaggc tgccggactt gtagccgggc
    32401 tcgccttcct tgggctggac gccggcgatc gtgccgccag tcgcgaggat cacgacgtgc
    32461 gcgagagcgg gcttggcggc gggagcggcg gccgtctgcg cggaggcgcc cggtgtcgcc
    32521 agaagggcga cgaggaggac gagggagaga gagacgatgc gtttcggcga catttgagga
    32581 ctcctatttc gtgagcatga cgatgacggg tccccacgcg gtgaggagga tgttcccgat
    32641 cgcgtagggg acggtgtacc cgaggacggg gagcttgctt cccgcctcgt cctggatccc
    32701 gcgcagggcg gccgtgacgg tgccggcccc ggcgcaggcg ccgaagagga ggacggggtt
    32761 catcttgagg acgtacttcc cgaagaagag cgctgcgatg tgcggggtga cggcgacgac
    32821 gaggccgacg aagagcaggc tcggccccgt ctccttgatt cccgagacga agctgggtcc
    32881 ggccgagagg ccgacgatgc cgatgaagac ggcgaggccg acggtgtcga agacccagag
    32941 cgcgggctcg ggaatccgcc cgaacgtcgg gcggacggac cgcagccagc cgaagacgag
    33001 gcccatgatg agcgcgccgc ccgacgccgt gaggctgagc gggaggccgc cgaccgtcac
    33061 ggacaggagc ccgacgaacc cgccgaggac gatgccgagg cccacgaaca cgacgtcggt
    33121 ctcgctcgag ggccgctcga catagccgat ggctttcccc gtgcgggcaa cgtcctcggg
    33181 cgcgcccgcg atccgcagaa ggtcgccgcg atccacggtc gtatgcagcg agaccggcac
    33241 gtcctcgccg ccgcgtacga gcgagacgag gccgacgccc cggccgtggg cctccgcgag
    33301 ctcggcgagc gtcttgccct cgatgtcgcg ggaagtcacg acggcgtcgg cgacgacgat
    33361 gggccaatcg aggagctggc ggtcctcgac ctcaacgccg aaagtcgaag aggaagagga
    33421 agaggaagat ttcttagaaa ggaatacggg gcgacgcgcg gcaagagcga ggacatcgcc
    33481 cgcggcgagg gcgatcccct cttcaccttc taagaagtcc ttctcttctt tccctcttcg
    33541 taccctcttc acaaaaaccc tgtcgggcgc gaaagatctt tcgaactcgc cgatcgtttt
    33601 gccggcccac tgtgggggca gctcgtacgc tcgtatgtcc cactggcggt acgcggactg
    33661 cgtgccctcc tcgcgctttt cccccgtgcc gcccgacagc gtcgcctcga gctttttcgc
    33721 ctcctccttc aggttcacct tcaggagctt cggcgcgagg cccgaaagga accacacgac
    33781 gaagccggtc ccgacgagat agctcaccgc gtacgcgacg ggaatctggt cgaggaggcg
    33841 tttcttctcg gcgtcggaga tcggcaggcg gttgatcgtc tcgcccgcgg tcccgatcac
    33901 ggtcgactcc gtgaacgccc ccgccatgag gcccgccgcg gtgccgcagt cgtacttgaa
    33961 gatcttcgcc gccgcgaccg tcgtcacgag gctcgtgacg cacaggacga gcgtcagcga
    34021 cacctgcgtg agcgcgttct tcttgagccc ccggaagaac tgcggcccga ccttgtagcc
    34081 cgtcgcgaag aggaagaggt cgaagaagac gagcttgacg accgggtcca ccttgacgcc
    34141 gatctggccc acgacgacgc ccgcgaggag agtgccgacg acgttgccca gcttgaacgt
    34201 gccgatcttg aggcgcccca cgagaaagcc gagggcgagc gtcaggaaga ccgcgagctc
    34261 cgggttctgg cggagggact cggcgatgtc atgcatgcag gcgttctacc tcagcgcgtc
    34321 acgaagagca gccagggagt cccgatgaga aggaggacga ggacgttgaa ggccgtcgtg
    34381 atcgtcccga gcttgtagag ctctccctgc gtcaggtagc cgctgcccac gacgaggagg
    34441 tttgcgctcg aagcctgggg cgtgagcgtc gagaggtagc tcgtcgagat caggagggcg
    34501 aagccgagcg gagcgaccgg cacgccgagc tgcactccga cgccgaggaa gacgccgaag
    34561 agcgcgagga cgtgcgccgt ctggctcacg aagaggtagt gaatgaggac gtaggcgagg
    34621 acgaggagga cgtacacggc gagccacggc aggccgccga gcgccgccgc gaggcgctgc
    34681 ccgatccagc ccatgaagcc gagcgtgttg agctggccgc tgatcgcgaa gaggatcgcg
    34741 aaccagatga acgtcgcgag gacgtcgccc tgctggccga tgtccttgag cgtcaggacg
    34801 cccgtcgcga ggaagatgcc gagaccgagg aacgccacgg cggtgaggtc gaccttgagc
    34861 gttcccgcga gcgcccacag cgtgaccatg ccgacgaacg tcaccgcgac gatcttctcc
    34921 tgcaggccga gcggccccat cgccgcgagc gccttcttcg ccgccgcggg cgcctcgggc
    34981 gtcgccttca gctcgggtgg gaagaccttg tagatcaggt acggaaggac cgcgaggcag
    35041 acgagcgtcg ggacgatgga ggcgacgagc catgagccga acgtcacgct gaggccgagc
    35101 gacttgccga tctcggctcc gagagggttg cccgccatcg cggtcagcca gagcgccgac
    35161 gacaggctga gcgtcgccat cccgcagaac atcaggtagg cgccggtctt cttgcgcgtc
    35221 gcctcgtccg gcttcgagcc cgagccttcg gcgagggaga ggacgagcgg gtagaggacg
    35281 cccgagcggg ccgtgtcgct cgggaacgcg ggcgcgatga cggcgtcgag gaagaagacg
    35341 ctgtacccga gcccgagcgt cgacttcccg aagagcgaga cgacgaggtg gccgagccgc
    35401 tgcccgaggc cgcacttcac gatcgcgcgc gcgacgagga acgcgacgac gatgaggagg
    35461 atcgtcccgt tcgcgaagcc cgcataggct ttcgccggct tgagaacgcc cgaaagcacc
    35521 gacacggcga ccgcgaggac ggaggccgtc aggatcggga acgcgcccac gatgaccgag
    35581 aagatcgtcg cggcgaagat cgcgaagagg cgccaggcct gcgccgtgag gccctccggc
    35641 acgggccaga accagatggc ggcgccgagg gcgatcgtga cgccgcgcca gacccatgtc
    35701 ttcgcggcgg ggctcacttc tgcgcctccg ccggggtctt cggaggcgcc cggcgcgcgc
    35761 ggccctcgac gttcagcgtc atcgcgtcga agtcgtcttc acccgactcg acgacgacct
    35821 tccacgcgaa cgtgaactcc ccttcgaggc cggcccagcg gcccgtgccg cccgtgatcg
    35881 tcgcggcggc cgtccggccc gtgccgagag gctgggcctt cagcgtggcg aagaccttct
    35941 cgcccttctc atcggtgaaa acggccctcc cgacgagaag cgtccccccg tcgtcgtatc
    36001 cgatcagctc tccgaagaag ccccggccga ccacgtcgcc cttcgtgacc acgaacgatc
    36061 ccgtggcgcg gacgatcgcg gcgcggcgcg cgccctcggt cggaagcgac gtccgttttc
    36121 ctgcgagcgc ccacgtcgcc gaaaagggcc gccactcctc ggccgccgcg gccgaagtca
    36181 ggaggtaggc cgccgcgccc gcgacggcga ggagggcggt ccgccgggat ctcatttctt
    36241 tccggtgcgc ttctgcccgg tcatggcggc cgggtcgagg acctcggcga tctgcttttc
    36301 cgtgaggacc ttcttcctcc ggatcagctc catgatcccg gcccccgtct tcatggcctc
    36361 ggcggcgagc gccgtcgcct tgtcgtagcc gatgatcggg ttgagcgcgg tgacggtgcc
    36421 gatcgagtac tcgacgtagt gcttcatgac gtccgggttc gcggtgatcc cgtccacgca
    36481 gtggacgcgg agcgtccggg cggcgttgat gaacatcgtc tgcgcctcga agatgcacgc
    36541 ggcgatgacc ggctcgaaga cgttcagctg gagctggccg gcctcggcgg acatcgagac
    36601 cgtcaggtcg ctgccgatca cgcggaagca gaccatgttg acgacctcgg gaatgacggg
    36661 gttgaccttg cccggcatga tcgaggagcc gggctgcttg gccgggaggt tgatctcgcg
    36721 caggccgcag cgcgggcccg aggagagcaa ccgcaggtcg ttgcagacct tcgagagctt
    36781 gatcgcgagg ctcttcatgc acgccgagta caggacgaag gcctgcgtgt cctgcgtcgc
    36841 ctcgatgagg tccggggcga gcgtgatcgg gaacccggtg atcttcgcga ggtgcttcgt
    36901 gcacttctcc gcatagccct cgggggcgtt gagccccgtg ccgatcgcgg tcgcccccat
    36961 gttcgtctcg cacaggacgc tctggatccg ctcgagcgcg aggacctcgc cctcgagcgt
    37021 ctgcgcgaag gcgttgaact cctgcccgag cgtcatcggc accgcgtcct ggagctgggt
    37081 gcggcccatc ttcaggatcg gcgcgaactc cttgcccttc ttccggaagg ccgcgatgag
    37141 ctcgcgcatc gccgcgacga gccggacgtt gccgagcgcc atcccgacgt ggagcgaggt
    37201 cgggtacgcg tcgttcgtgg actgcgagca gttcacgtgg tcgtgcgggt cgcaggcctc
    37261 gtagtcgcct ttcttcttcc ccatgagctc gagggcgcgg ttcgcgatca cctcgttcgc
    37321 gttcatgttc gtcgaggtgc ccgcgccgcc ctggaacacg tcgaggcgga actcgtcgtg
    37381 gagcttgccc gcgatgatct cctcgcaggc gccctcgatc cccttcagaa tcttcttgtc
    37441 gaactgcccg cagtcgaagt tcgcgcgcgc ggccgcgagc ttgaccatcg cgaacgcctt
    37501 gatgaggtcg gggtacagcc tcagctcgac gccggagatg tggaagttct cgcgcgcgcg
    37561 ggcggtctgg acgccgtagt acgcgtcggc gggaacggcg aggtccccga gcaggtcgtg
    37621 ctcgaggcgg gttgcgcgtt tggacataga tcctcctgaa gaccgccctc gttgagagcg
    37681 aaggcggtgc cagcgcccgg gttcgacgac cttcgaagaa gtccctctgc gggctggact
    37741 tgcggatgcg ccgctcacgc gtgtctccgc cgtccatgcg ccgcttggcg ttccgtcgtc
    37801 agggatggcg gcatgtcgcc agggccgttc aagcgatgtc cgggccaatc cgcgcgctcg
    37861 ctcgacgggt atgattccgc cagccaactt tccagcagca ggagtgggcg caccatgaaa
    37921 cgaacgaaca atgcgaacgg actcttgggc cgtggctggc gaagcgccat cgcgattgcg
    37981 tgcgcgctcc ttctggtacc gggggaagga atcctcctgg cgcaggaggc gtctcccccc
    38041 gccgcagcgg ctgaggcgga cgatccgccg ctcacggccg accagctcga ctcgctcgtc
    38101 gcgccaatcg cgctctaccc ggacgatctc ctcgcgcagg tgctcgcggc gtcgacatac
    38161 ccgctcgaac tcatgcagct ccagcagtgg atggcgaagc acaaggacct caaggacaag
    38221 gccctcgccg acgccgtcgc gaaggagaac tgggatccct ccgtcaagtc catggcggcc
    38281 gtccccgacg ctctcaagcg gctcacggac gacatccagt ggacgacgga cctcggcaac
    38341 gcgttcctcg atcagcagaa ggacgtcatg gactcctgcc agcggatgcg caagaaggcg
    38401 aaggacaagg gcgcgctggc ctcgaacgag cagatgaagg tcgagacgaa ggtcgtcgag
    38461 acgaaggaag tcatcgtcgt ccagtccgcg aaccccgagg tcatctacgt gccctcgtac
    38521 aacccggtgt acgtctaccc gccgccggtc tacccgtacc cgccggtcta ctacccgccg
    38581 tacacaccgg gcgccgcgtt cttctcgttc tcggtgggcg tcatggtcgg cgccgccttc
    38641 tggggcggct cgtgctgcca ctgcggctgg ggcggcggca acgtgaacat caacgtgaac
    38701 aacaacttca accggaacac gaacatcaac gcaggcaacc gcggccaggg cggcggtagc
    38761 tggaaccaca acccgagcca ccgcggcggc acgccgtacg gcaacaaggc gagcgcgagc
    38821 aagtacggcg gccagacgcg cggccagcag gcgagcacac ggcagcaggg cgggcgcgga
    38881 acgtcgggcg cgggcgaccg tgccggcgga agccggggtg gcccgagcgc cgggaccagc
    38941 gacagg
//
LOCUS       DAOMFY010000007        38646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_332_length_38646_cov_9.241611, whole genome shotgun sequence.
ACCESSION   DAOMFY010000007 DAOMFY010000000
VERSION     DAOMFY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 38646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38646
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_332_length_38646_cov_9.241611"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLB01_01400"
     CDS             <1..782
                     /locus_tag="PLB01_01400"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQQ75985.1"
                     /translation="WVDDDEEYQRIVEFLDQVQPSLVSKVKLYRKSTPLFEEYGVESE
                     IENALKSKVWLKSGGYIVINQTEALVAIDVNTGKFVGKKRLEETVVKTNLEAVQEIVR
                     QIRLRDLGGLLVVDFIDMEEEGHRQELFTVLETELKKDRSKNRMLPVSEFGLVEITRK
                     RQRQSLERALTMPCPYCAGSGRTKSVPTIVLEIRREIAKRVEEGEAHSLMLRVHPEVY
                     RSLVGEERRLVEELEERIGMPIHVQSDEHLHHERFDIVLEA"
     gene            937..2109
                     /locus_tag="PLB01_01405"
     CDS             937..2109
                     /locus_tag="PLB01_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ75986.1"
                     /translation="MTAERLFTRKFFLMCGFTFTVFLSLFQLLPTAPFRILELGGSTT
                     AAGLFLGLLTYASALSAPLTGALADRIGRRRMLLVSSVAIAFFALAYALAPRWEVLVF
                     LVFFHGLFWSGLLSASAAYMTGLIPNERRAEGIGYWGMATVLATAFAPALGLAVYGLG
                     WTSLCASVGVLNLAMAAIAWNLEPDPPRAAPHGPFFTRDLVEWRVLAPSLALFLCSFG
                     YGGVTSFVAMWTEKNGLAPRGLFFTVFSLTVLVLRPFLGRFADRVGPRRVMFPLLGTT
                     AAAFALLAVATTRPLLALAAFLFGAGFGNVYPVFAAHVTRHVLPERRGAAFGAILAAF
                     DVGIGTGSIALGAVIGRAGFSAAYGTAAVLAALSVPAFLLLDRRAFPPGAALSSTP"
     gene            2106..2393
                     /locus_tag="PLB01_01410"
     CDS             2106..2393
                     /locus_tag="PLB01_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75987.1"
                     /translation="MNTAPRLSRFAWKEFLRGPLGTPIVLARGIRPVGRLMPIRTSAL
                     RTWRRVSALPLPASIRKALYRAVYVLPLGPGDPATLTRVVARHRAFFAARA"
     gene            complement(2423..4894)
                     /gene="lon"
                     /locus_tag="PLB01_01415"
     CDS             complement(2423..4894)
                     /gene="lon"
                     /locus_tag="PLB01_01415"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQQ75988.1"
                     /translation="MRGVEVLMTETLTLPVLPLRNAVLFPGVSTPISAGRGGTVRAIE
                     AALRGDALVFALSQREDVEKVTPEGLYTIGTIAKVSSVQRGLGGMRLLLEGQRRGIAM
                     RVTEKKGGMLEAVVVPAAEMAPADAKDPVFLALHKEARERAAELGEKMGLPEEAVAQF
                     LEQAEEPGRLADLVAGYIDIPMADRQQLLETLSVEDRLRRVLVAVQRQIGVLSAQEDI
                     QSKVKEQIGDRQREMLLREQLRAIQKELGEGEGKDAADLEELKKKLAAHPLTEEVRKE
                     VDRELARLERIGRESMESQVIRTFLENVAELPWGVRSEDKLDVNEAARILDEDHYALG
                     DVKDRILEFLAVRQMRAKAEKTEAQAAEGAENALKKDMSTGKGRILLFVGPPGVGKTS
                     IAKSIARAMGRKYVRISLGGARDEADIRGHRRTYVGAMPGRIVQGLKQAGTKNPVFLL
                     DELDKLGVSFQGDPASALLEVLDPAQNDSFTDHYLGVPFDLSEVLFIATANFIQNIPG
                     PLLDRLEVVEFSGYTEREKLAIARQYLLPRQLAENGLTAAQLVLTDAAIAEIVTSYTR
                     EAGVRQLEREIGKLARKVARRIAAGDVAAVAVEAGDVFPLLGKKRVHPEKKIVTDQVG
                     LATGMYYTPAGGDIMFIEAMLMKGKGEVVLTGQLGDVMKESARAAWTYARSHAAELGI
                     PDEAFERDLHIHVPAGAIPKDGPSAGIAMATAIVSALSRVPARHDIAMTGEITLSGRV
                     LPIGGLKEKVLGAVRAGIRTIVLPKDNEPDLADLPKEVRDAIEVHPVDGLGEALAVTL
                     RGASFRGGHFVLRDGAVPSAGTPAH"
     gene            complement(4980..6128)
                     /locus_tag="PLB01_01420"
     CDS             complement(4980..6128)
                     /locus_tag="PLB01_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQQ75989.1"
                     /translation="MPRPPRTSPAVAALHGNPYSSLLARLATHEGEVFPLHVGDTWRK
                     APAGCRPEELGPGLPGINRYTDVWGLPELRDAIATRASAQTGAPVARANVLVTGGATA
                     GLMAAVAALVAPEEEVLLLGPRWPLIEGHVRLAGAVPVEVPLFLGDLSPEAVAAAVRV
                     RVTERTAAIYVNTPSNPTGRVLPPGSVDALAHVAREGNLWLLADEVYELYAYGGAHRP
                     AYAAAPERTIAAHSLSKAWGLSGARCGWLVGPEAALAEIVKVGAHTIYSAPTASQHAA
                     LRILAGAGDAWVAEARAEYAATGREAARRLSQPAPEGGMFLFLDVAAALDERGLLGLL
                     EDVAGEGLFVAPGPSFGPYPTHVRLCYTAVPPDAALRGVDILARRLGR"
     gene            6189..6980
                     /locus_tag="PLB01_01425"
     CDS             6189..6980
                     /locus_tag="PLB01_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HQQ75990.1"
                     /translation="MGALADLPAYLRRIGLGAPPPANAEGLAALHRAHAGAIPFENLD
                     ILLGREIRLDLPSLEAKLVAARRGGYCFEQNTLFAAVLADAGFRVTPLAARVRLGGRT
                     DGPRTHMLLAVRAEGRDWLCDVGFGGGGPWEPLPLEPCGEIAQGLWRFRLVEDGGERV
                     LQNRDPSGWRDLYGFTLEPQRPADYAVANHYTSTHRDSSFTKVAVAQRTSATGALVLR
                     GDVLQTMRPGEAPAETPAPEGDALLDLLRERFGLDFPAGTRFRPL"
     gene            7044..7463
                     /locus_tag="PLB01_01430"
     CDS             7044..7463
                     /locus_tag="PLB01_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQQ75991.1"
                     /translation="MVRTDPHPPEVFELLRRKTAATRIAVVGASNVPYKYGNVIVKNL
                     AAKGYTVLPVNPREKEIAGLPAYPDLASVPGPVHIVDVVTPPAVTHGILREAARLGLP
                     AVWLQDGSYDDEALAIAAAAPYKTVYEACIMVASNFG"
     gene            7553..10615
                     /locus_tag="PLB01_01435"
     CDS             7553..10615
                     /locus_tag="PLB01_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQQ75992.1"
                     /translation="MKKKTFAAALLLSLVSRPAPALDKVRVETRVDAAFAKYGLTGRG
                     VIVALLDRGIDWKNNDFRNADGTTRIAYMLDFTDNTGAATSGYGRGTLYTRAQIDAAL
                     SGGPTIPFRDAVGHGTPTAGTPTGNGRNVAKYRGIAPEATIISVKVTSDGAPAHDGEA
                     AEAGFNDATVFPLAIDFVKAKAQELGMPAVMLLNLGSQGGPTDGTSSLARKIDATVGP
                     GIPGLVFVSGPGDEGGSANRAGGNVASGSTQSIRIHKGQAGGLLFDLWYPGADRFDVT
                     VQTPTATYGPYVSPATNADSATVTNSEFTYYQQGSSRVFYGAQNGKREIFLNISGPVG
                     DYTITLKGTAVTTGRFDATINPSGHGGSNYFLDHVAPGSIWDGATARYNICPGDYVIR
                     TSYVDIDGITRTITGNGSVGEIWPGSSIGPTFDGRLGIDVVAPGDSLFSVYNPKSYWA
                     TSRFNLIQDGLGFYGRANAVSAAAPIVTGIIALMLQANPTLDAPTVKSILQRTARGDS
                     FTGAVPNTTWGYGKVDALAALDAVNTTTLFVPIALSSSGANGSFYTTELALTNRGTTD
                     ATITYAYTAAFGGASGAATETLGAGRQKTYADTITSLRALGIPLGDAGNRGGTLQVTF
                     GGLSSPSAAAATVRTATPVADGRAGLAYAGLPSSRLLSTPVWLCGLRQNATDRSNVAV
                     QHAGGPSDGNVTLRLTVVSGDPANPGSLALPDVTLSPGGFSQISGILASNGLSFSNGF
                     VKVERVSGTAPFYAYGVINDQANSDGSFVEPVPASPASAIGGVTLPVVVETSTFSTEL
                     VLTNFSGSSRTLQCTYVAAPLPGGQVTFNLPLLPNEQQILPAFVQVLRSRGVVPGGPG
                     ATFAGALFVRDASGDLRGVSIGARTSSAGGGGRYGLFYSAVPNGAEATTVAWLYGLQQ
                     NADNRTNLALVNVGSTDASTDTFHIDLYDGATGLKAGETDATVAAKGFFQIGTILAAY
                     APGVTSGYAKVTRTSGNNPFVAYAVINDGGQPNQRSGDGAFVSAVVGP"
     gene            complement(10628..11209)
                     /locus_tag="PLB01_01440"
     CDS             complement(10628..11209)
                     /locus_tag="PLB01_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ75993.1"
                     /translation="MASDILRERGQAMEAQWARQRDEELLMKLRERAKLEEVSAALAR
                     KLRVDDPALRQRIIDLGITHDTGPALFLAPLVQIAWADGDVTERERETILGMAAARGT
                     GPGSPAHTQVLEWLKKQPSAALYDAAVEVLKVGISVLPEEEKKQRVDAYADSFRRVAE
                     ASGGGLGRVFGRANAISAEEEALIARITAALRA"
     gene            complement(11273..11349)
                     /locus_tag="PLB01_01445"
     tRNA            complement(11273..11349)
                     /locus_tag="PLB01_01445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11313..11315),aa:Arg,seq:ccg)
     gene            complement(11368..11443)
                     /locus_tag="PLB01_01450"
     tRNA            complement(11368..11443)
                     /locus_tag="PLB01_01450"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11408..11410),aa:His,seq:gtg)
     gene            11532..12704
                     /gene="pheA"
                     /locus_tag="PLB01_01455"
     CDS             11532..12704
                     /gene="pheA"
                     /locus_tag="PLB01_01455"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQQ75994.1"
                     /translation="MDALDELRRRIDETDRQLLETLALRLKFVAEVGRLKSKGVPFLR
                     DHARESALLAKVEAQAKELGLDAFRTTEIFREVIAMSVKAQEEALLDRKAAERSLGNA
                     HRVAFQGAEGAYSHIAARKYFGASATEFAGFPTFAAALAEAERGDAGYAFLPIENTTA
                     GSINETYDLIRHTDLKIVGEEILEVRHCLLALPGATLEGIRRVASHPQALAQCGRFLA
                     QLAGASAVPYIDTAEAARFVKESGDRSLAAIASEEAGALHGLAILARDLADQEENWTR
                     FVVVSAIPIVLDPRIPAKTSLVFSTPHRHGALAHCLNVLDAHGLNLTKLESRPVPRHP
                     WEYLFYVDFEGAAGEDAASRAVAALSAECPYLKVLGSYPARTTKKGRVDRLPEAPP"
     gene            12701..13843
                     /locus_tag="PLB01_01460"
     CDS             12701..13843
                     /locus_tag="PLB01_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQQ75995.1"
                     /translation="MIVLVDETTAKNAAAEKELLDALGRLPGIEVRVHKVRGAEKTVT
                     EVYLIGDTHRLAVEDVAVLPGVERVVRVSQEYRVIGRHKDDRRASGFTYNGVEFSQDN
                     LHVFAGLCAVDTPENADAMFAALASFGQTTTRMGAYKPRTNPYAFQGHGKGCLPWVFE
                     KAGLHGIRVIAMEITHESHIDEIAECLEKTGQPTGVMLQIGTRNIQNFELLKTVGRQT
                     VFPVLVKRGFGITLNESLNAAEYLASEGNSQIVFGLRGVKTNLGDPHRNLVDFAHVPV
                     VKRLTRLPVCIDPSHSVGSREKAPDGIPDLMHVTAQGVAAGANMVLVDFHPHPAHALV
                     DGPQALLLSELGWFLEDVAVARAAYEKRRTLALSHAAGAPVSAATA"
     gene            13840..14565
                     /locus_tag="PLB01_01465"
     CDS             13840..14565
                     /locus_tag="PLB01_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HQQ75996.1"
                     /translation="MTAVFREAARVRASEIGPAGTMRLPALLDLFQEAAGNSATSLGF
                     ASDTLLAEGKTWVLSRFHLRLLALPAWRDEIVVETWPSGVARVFALREFRATGTDGRL
                     LAEATSAWLLVAVPSKKPLRPPAEIAAAAAGTPPRVIGDAFEPLAGPGPDAAKGPQTR
                     IGRFDLDLNAHANNVAIFRALLEALPHADPPFDLEVEFRGEAFEGDFLEGRTDEGGGA
                     RRVALVRPADGREIARAVLTVRG"
     gene            complement(14549..17485)
                     /locus_tag="PLB01_01470"
     CDS             complement(14549..17485)
                     /locus_tag="PLB01_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQQ75997.1"
                     /translation="MTHDRTPSRLLALAFLAALPAVAQPFPQGLFGEMRWRMIGPFRG
                     GRTVAATGVPGNTNVFYMAPNNGGVWKTTDAGRTWVPIFDAQPSGSIGALAVSWSRPD
                     RVWVGSGEGLQRPDLSVGNGMYRSDDGGKSWVRLGLADGQQIAAIAADPKDADRAFVA
                     VLGHPYGANETRGIFRTRDAGKTWEKVLYRDADTGGAAVALDPKDPNVVYAALWSSRL
                     GPWENGQWQGPGTGLFKSTDGGTTWRAIGKGLPAHAQGGGRIGFGIAPSEPKRVYATV
                     DALDEEKESGGGIFVSDDAGETFVKVSGEKRLWGRGSDFAEIKVHPGSKDVVWVANTS
                     AYRSKDGGKTFEPVRGAPGGDDFHTIWFHPENPDVILLAADQGAIVSVNGGATWSSWY
                     NQPTAQLYHVSTDDRFPYWVYGGQQESGSVGIASRGDDGAITSRDWHPVAAEEYGYVA
                     PDPKDPDVVYGGKLTRYRHSTKEVQDVSPAPLRGTYRFLRTAPVIFSPADPRLLFYAG
                     NVVFRTADGGRHWDVISPDLSRERTAVPASIGVFRTKDLETMKRRGVVYTLAPAYKSA
                     DVLWAGTDDGLIHRTADGGKTWADVTPPGLTAWSKVSMLAASPHDDLTAWAAINRIRL
                     DDMKPHVLRTHDGGKTWEEVVHGLPDDPVNAVREDPVRKGLLYAATERMVCVSFDDGA
                     NWQPLRLNMPATSVRDLVVKGDDLVVGTHGRSFWILDDVTPLRQLTPAVAAARAHLFE
                     PQKATRIRRSRWTDTPVPIEEPAGENPPDGAILGYVLGVSSATPVVLEIRDAAGGVVR
                     RYASDDAPEAKVEPLVVTADWARPALTLPRSAGHHRWVWDLRYAPPPVSSREYPISAV
                     PSRTPPEPEGPLVLPGTYTAALTVDGRTLTRTLTVGMDPRVKASPGDLAAQHAAAKRL
                     AADLARLAEARKKGADAGKAESLEGKLTRLYGMVQESDDAPTPQLLAAVDEVEKDFAG
                     LFSRAR"
     gene            complement(17488..18798)
                     /locus_tag="PLB01_01475"
     CDS             complement(17488..18798)
                     /locus_tag="PLB01_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ75998.1"
                     /translation="MPDSSAPGSALAPPPPAYRWAVLLTCSLAMFGVYYVFDALYPVT
                     PLLEKAFGFTGAQIGLLDTAYNVAALLTLIAGGVLIDRIGVAASAILFGAIGAAGGIL
                     IAVLPVLVPGAPWAGMMAGRFLLGIGSELFIVAATTVIGRWFKGKEISFALAIQLLVA
                     RLGSWAADKSPDLFKPLFATWQPPLLLAAALGVVWLVFAIVYSGLERRAARVYAVSRP
                     GMTDKLVWSDLVRFDLGYWWVVGLCVAFYATIFPFRTFANLYFIEAKGLSPEAAGNLK
                     SILPLLSMIGMPLFGLLADRIGKRALLMAVGSALLVPPFFLLPFTGVSPTLLMGMLGL
                     AFALVPAVLWPAVTYLVPEARLGSAYALMTFCQQVGWAGMSWGLGLLKDASHASAERP
                     EGWTPVVVTLGVLASVGFVFSFLLWRSERGPRRHGLEAVTPPAK"
     gene            complement(18858..20042)
                     /locus_tag="PLB01_01480"
     CDS             complement(18858..20042)
                     /locus_tag="PLB01_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQQ75999.1"
                     /translation="MNSHARLVPLLAAVAIAAGLALSPPASAASGAPITVMTWNTYFG
                     FDEGPLFGAAASGDPELIAAAVSDVWAQVHAADFRVRAAAIAKKVKAARPDLFAVQEA
                     VQYVRLDGTTFEPMEVIDHLAILVEALAAEGMFYEVQSVVEDTNVMLPDAEGHVVMLL
                     DRSAILVRGEPSPGKPRLSVSNPKSGTFALLAELCIVPAADGSCEVPLVIKRGWASVD
                     VEDTGSRFRFVVAHLEDVPAPVANEEPSPLEQLQLAQAAELTLGETGPFATDLRVVLA
                     GDFNTDAATGWPTYRFLTAPAPAGPGLKDAWLALHPKDAGLTWGPNPDLLAPFPAFTQ
                     RIDFVFLKGDGFDLVSANVVGNTRGDVAPAGLWPSDHAGLVVRVTVDRPARSVERFDM
                     IP"
     gene            complement(20077..21147)
                     /locus_tag="PLB01_01485"
     CDS             complement(20077..21147)
                     /locus_tag="PLB01_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQQ76000.1"
                     /translation="MKSRPRLIAFFAAAAATLGVAAAPAASADGPKPVKVMTWNLFYG
                     FDDGPVIAAAMSGDPALIVPAATEAWRQIHATDFHRRVRDIALRVRLEAPDLIGVQEA
                     VKYTQAGSTGIVEELDHLEILVAALHDAGIPYEVVSSVDTLAIGLPVLDAAGELTMVS
                     VQDRVAVLARSRNAKVAVSNPQSGIYQVILGPEVGFPFPLPQGWASVDVEASGHRFRF
                     VTTHLEDASADPAVLPIQEAQAVELVNRHAADGLPVVFAGDFNTDGFRKWPTYKLLTG
                     PLHLTDAWKAVNHNAPGLTWGQSPDLLNPFPRLTQRIDLVLLSGRFDVTDADVVGARL
                     LDRLPAGMWPSDHAGVVVTLVP"
     gene            complement(21252..22556)
                     /locus_tag="PLB01_01490"
     CDS             complement(21252..22556)
                     /locus_tag="PLB01_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ76001.1"
                     /translation="MAHATARTGYRDLVDRLNRFPQGAPPSQLLDRILQILFTEREAS
                     LVSALPIKPFTLETAARIWKTTPAEAQNTLDALASRALLLDATHGGVTRWVLPPPMAG
                     FFEFSMMRLRGDIDQKTLAELFYGYLNVEEDFARELFARGETQLGRAFVNEDALPEDE
                     PLEVLDWERASEVARTSRDVGVGLCYCRHKMSHLGRACGAPMEICLTFGGTAAALVRH
                     GHARKSDSAEALDLLAQARGQGLVQFGENVRRQPAFICNCCGCCCEALIAQRRFGFLK
                     PVHTTGFVPEVDAERCTGCGRCVEACPVEAMGSVSANDPKLPKKRLARLDERICLGCG
                     VCVPACRTGGLRLVRREKRVIPPLDTVHRTVVMAIERGRLAEVLFDDRTLASHRILGA
                     VLGAIVKLPLVQRGLASEQVKSRYLEALIERLGPGRAGARPA"
     gene            22691..23020
                     /locus_tag="PLB01_01495"
     CDS             22691..23020
                     /locus_tag="PLB01_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76002.1"
                     /translation="MRKAPVAVALAILLAAALPLTGAGRDCSKLTEIQIQAKSDKTIY
                     TGTSPATYRVCVCDAEPVVELVADGKVVATVTGGQCGDVTGRKIEVHASDDRGVTVGY
                     TVVTPAR"
     gene            23080..24456
                     /locus_tag="PLB01_01500"
     CDS             23080..24456
                     /locus_tag="PLB01_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQQ76003.1"
                     /translation="MPFRTIIEPFRIKSVETVKETTPEYRRRAIAEAGYNVFLLPAAD
                     VLIDLLTDSGTGAMSAAQWGALIQGDESYAGSRSFYRFRDVIQDLTGFKHVIPTHQGR
                     AAERILFHTVLKKGDVVPNNNHFDTTRANIEVEGAEARDLVIAEGRVPSLIHPFKGNI
                     DLAALEKTLVEDGDRVPLVMVTVTNNSGGGQPVSLENLRGVRALCDKYRKPFFLDACR
                     FAENAYFVKRREKGQEGRTPKAIAQEMFSLADGCTMSAKKDGLANIGGFLAMNDDAWA
                     ENARNLTILTEGFPTYGGLAGYDLEAIAQGLVEVVEEHYLQYRIRSIEYLAEKLEAGG
                     VPMIRPSGGHAVYLDARALLPHIPPARYPGIALVNALYEAGGIRGVEIGTVMFGMKPD
                     GREEPGPMDLVRLAFPRRVYTQSHVDYVSEVVLAVARDAASLPGYRITSAPRVLRHFT
                     ARFEPIAP"
     gene            24453..26006
                     /locus_tag="PLB01_01505"
     CDS             24453..26006
                     /locus_tag="PLB01_01505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQQ76004.1"
                     /translation="MRAILSLAALLVAAAPLARASADVPPAAFADPGRRAKLLAALPA
                     VEKAVAAAVEKKKFPAAAFGVVVDGDLVLAKGWGAQGPSGGAVDADTVFRIASMTKSF
                     TALAILRLRDEGKLSLDDPVSKWVPELAGMPKATADSPEITIRHLLTHSAGFPEDNPW
                     GDRQLAQSDAFLAAMARAGIPFSNAPGVAFEYSNTGYALLGRVVARASKMRYRDYVDA
                     NILKPLGMTSTFWEASAVPPGRLVTGMRLEGETPVDEPLLGDGAFGAMGGLFSTVRDL
                     ARYTAFFLSAWPPRDDPDAGPVSRASRREMQQAARTTGASVKIAADGKPRLTAGGYGY
                     GLNDRATCQFRHVVSHGGGLPGYGSLMLWLPEHGVALVALANLRYAGWSGIFNDALDA
                     LAGGGGLKARVPQAAPALRAAYGAVNRLYTAWDDRAFDALAADNLVLDTPLEKRRREF
                     ADLRAKHGACVPRALEAENALRGEWTLACERGEIALKITLSPTQPPKVQLLEAKSDPA
                     RAADEPCAP"
     gene            complement(26080..26355)
                     /locus_tag="PLB01_01510"
     CDS             complement(26080..26355)
                     /locus_tag="PLB01_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76005.1"
                     /translation="MKKTTTAVAAIAALVMGSSAFATMDIMNRYKKGADGKDIKDPNA
                     SCASCHKEKMPKKETAALNELGDKVKAAKGKDGKIDWTKVPVVEAKK"
     gene            complement(26567..27718)
                     /locus_tag="PLB01_01515"
     CDS             complement(26567..27718)
                     /locus_tag="PLB01_01515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ76006.1"
                     /translation="MPERADVAIIGAGVIGTSVAFQLARLGATRVVLLEKESLPGTGS
                     TSKANGGIRAQFTTDVNVAMSLASMEILDALAGEIGQPPMYNKAGYLFLTGDAGKLAA
                     MESAAAFQRARGVAVEVLDPAAVRARAPYAEGPIAGGTFGPRDGFIDPGGLTNFFLRE
                     AARAGVRTLYGAGVRAIARAGADYRLETEAGAVEAGAVVIAAGPWTGRVAALAGLDVP
                     VEPVRRHIFVSGPCPHLPPVIPMTIDADSGVLVRREGNRVAIAWSNADEPPGFNAAFD
                     PDFFLRFAEPLEARFPSIAAAGLDMRRSWSGLYEVTPDHHAVVGAVPEMAGLFVASGF
                     SGHGVMHAPAAGRAIAELILRGRCETVDVAPLALDRFRRGALIHETMVL"
     gene            complement(27786..29543)
                     /locus_tag="PLB01_01520"
     CDS             complement(27786..29543)
                     /locus_tag="PLB01_01520"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HQQ76007.1"
                     /translation="MEKYSVRHDSKGAARLEVPYRGTRLLRHPMYTKGTAFTEEEREA
                     FGLEGLLPHAVSTMEQQERRVYANISRKADELEKFIGLASLQDRNEHLFYRTLVDHIE
                     EFLPIVYTPVVGRACQEFSHIFRRARGLWITPGHRGRIYEVLGNAPFADVRLIVVTDN
                     ERILGLGDQGAGGMGIPIGKLALYTAAAGIHPAQTLPISLDVGTDNESLLNDELYLGW
                     RFPRLRGAEYDSLVEEFVQAVKRRFPKVLLQWEDFKKVNAFRLLDRYQKSITCFNDDI
                     QGTAAVAVAGMMAGCRVTGIPVKEQRVVILGAGAAGIGIARLLRDTFSRAGVSGEALT
                     LATANLDSQGLVVDDVEIKDVHKRPFAWPAALAAKYGLGTGAPRDLLAVVKAVKPTML
                     VGTSGEPGTFTEAIVREMAKHVERPVIFPMSNPTSKTEAKPVDITKWTDGRALIATGS
                     PFDPVPHAGKKIVIGQGNNAFIFPGVGLGVLVSEAKEVTEAMFAAAAKALADEVKASD
                     LASGSLFPSTAEIRRVSAAVAVAVVKVARDSGLGKAIPDAEIPKAVAATMWNPAYLPT
                     VAVPLRSDVHAEDFVPALV"
     gene            complement(29605..30519)
                     /locus_tag="PLB01_01525"
     CDS             complement(29605..30519)
                     /locus_tag="PLB01_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76008.1"
                     /translation="MKRPMLFARIPALFLAALVLTSCAWKKDLEKAQAENKALTEEKA
                     RAQRELSAAGAATAEMQATLDEVQKGLEELRVKELKAVKTSIAVAQEGKTNTAQREEL
                     KAEIADIRKAVAANLAKLATLQKSLKASDAKVTSLERLVGELRKQLEEKEATIAALEE
                     KTKELTKTAEELRGTVKEKEETVQQKEAVIADREAQMATAYVLIAPKETLKKAGIVEK
                     KGAVLGLGGNYKQTGQFDENLFKPVDTRKVTTFDIPAAADKARVLSDHPKDSYTLTAA
                     GEKASTLTVTDAARFWKASKVLVVMLPN"
     gene            30613..31509
                     /locus_tag="PLB01_01530"
     CDS             30613..31509
                     /locus_tag="PLB01_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQQ76009.1"
                     /translation="MKRIFVGFAALWLLAASPGLAQDAEGCKDHPLFTRFPNTHIIDC
                     QSSQFDLRKFPVGQQGKGDETLKSVEVEGPVRWLGYELNEGATPPSGLQIMRNFENAA
                     KKAGGTVEGQYPGWCKAMYDPEGMPRMGNGCTSYALTLKFLRGGKETWVFLQAGENDG
                     SYVMTVSEREAMKQDVTVNELADKLTKDGFVTLYVNFDTGKSTIKPDSAKTLDDAAAA
                     LKVAATLRVEVAGHTDNVGAPEANQRLSQERAQAVMAALVGRGIKADRLTAKGYGQTT
                     PVADNRTEEGRARNRRVELVKK"
     gene            complement(31525..32271)
                     /locus_tag="PLB01_01535"
     CDS             complement(31525..32271)
                     /locus_tag="PLB01_01535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ76010.1"
                     /translation="MRDPYVPLAPRYDRMAADPGIQAIYREFRRALTTAAAAHRLRPR
                     VIVDLACGTGNTAIPWAGARGRTVIGVDVSEAMLRVARRKSRRVRWVRQDLTRLSLDV
                     TADVATCHFDALNHILDAKDVQRIFANTARLLRPGGLFLFDLTTRSWLEWLSTSEKLF
                     EAGPDVFMATNAFDRKTGIATFNQLWFVKQGRLYRKILVTVKERSFEDAEVRRMLRVS
                     GLRLETIRVIQRMKGRPVRKLYVASRRGAS"
     gene            complement(32325..32744)
                     /locus_tag="PLB01_01540"
     CDS             complement(32325..32744)
                     /locus_tag="PLB01_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQQ76011.1"
                     /translation="MTGTLEQEILAQEERLTRATREIDLDALDRIYADDILFTGLTGA
                     TCGKSSLMDEARRGAAERQAAAGGAKPFVASYAKDDVKVVAHGDTAVTSYRFVVTIQS
                     QGQEIKRGYRTTNVWMKRPPGWQVMAGHTASLDVVPG"
     gene            32845..34614
                     /locus_tag="PLB01_01545"
     CDS             32845..34614
                     /locus_tag="PLB01_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQQ76012.1"
                     /translation="MAERESYRFGDMTLDVAERRLSRGGRSVPLAPKGHDLLVHLVRN
                     AGRLVTKGELLEHVWAGSFVEEGILSVHVSALRRALGDDRRSRRYIETVSRAGYRFIA
                     PVQDDGARDGRSLAVLPAWPAGPGTSDAEQSVGLALADAVIARLGSLTRISVRPTRAV
                     RSYADLQPDPAAVGRSLGVDAVIVCKFEKTPERIRVAAELLRSRDGVRLWSGAYDELP
                     GDGTSIPDAMAEALAAHFAATPLRGGSAGVFGNWADVRRSVSRPPCRLEVYELIGRGR
                     AHLLAFSRTETPKAIAAYEAAIGLDPAFAAAHAGLAMAFCQQAELRLVPQAEAYARAK
                     DAALRALAVDDACADAQVALAAVSFLGQWDWVGAERALQRALEINPNHTEATVLYGRL
                     LDTLGRLEEGLEMKMRALERDPLSPLVHQAISMSYWHQRRYEDSIRWANKTLELDAGH
                     LVAREHLAGAYWALGDFDRHMAENVKHAESFGVAAEALEPIKQAYARGGRTGLVRWLL
                     ETQGTHLPAIQLALLHGELGDLEAAMRHLARAIDGHESCLVDLAVAPQWDRLRESPDF
                     QVCLARMGLLARDPGSTSRQNRP"
     gene            complement(34690..35400)
                     /locus_tag="PLB01_01550"
     CDS             complement(34690..35400)
                     /locus_tag="PLB01_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76013.1"
                     /translation="MKADRIGPNAFTLEFETEEELREEHRTNLSQRGLRLPVSERPAP
                     FTPVEVTLRGPSGGEAKVKGTVVAPLPDGVALHVEGDAGGLLEALLASPAPPPAAPGN
                     VSDWDRLRGMSRTEKLLHAARADRNDRLILVQDGDPQVLYALLKNPRLTVDEVVRVAK
                     SATLTYQTAELLLKTSQWAANAEVKAALVHNPKTPQVFALRILPGLPEAEIKAIARGA
                     ATSMALKQAALRRLHGGG"
     gene            35553..37100
                     /locus_tag="PLB01_01555"
                     /pseudo
     CDS             35553..37100
                     /locus_tag="PLB01_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582709.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(37138..37815)
                     /locus_tag="PLB01_01560"
     CDS             complement(37138..37815)
                     /locus_tag="PLB01_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HQQ76014.1"
                     /translation="MFETLNLAVQVVFVGEIAARLFAQAPRIHRFFLDGWNVFDFTIV
                     ALSLLPVAGPFATVARLARLLRALRVVSALPEMRLIVATMLRSIPSLANVVALLGIIL
                     YVYAVLGVHFFGGADPGHWGTLPRAGLTLFEILTLEGWVDLMDASLAATRWAWLFYVS
                     FVVLAVFVVINLFIAIVINNLETANGEQKLAAGSEREPLARRLAGMRAELEAIEAELR
                     KPVSGNG"
     gene            37879..38598
                     /locus_tag="PLB01_01565"
     CDS             37879..38598
                     /locus_tag="PLB01_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HQQ76015.1"
                     /translation="MTRSANARVAGFTFLFYIVAGITSMILNGRASGGPDPAARLQAI
                     SRHPTEMAVVVILLLFQCFSALVLAVTLHALTRETDRDLAMMGLLCRAAEGILSGAGI
                     ASTLAVVDLARTTGVGGSDLPARHLLGAHLLREDIALTATFFAVGSLLFSLLFLRGRL
                     IPAPLAWLGVAASVLLVVELPLQLAGFVHGPVTRLVWLPMLAFEVPLGVLLLVKGVRA
                     PATPATAPLGLAGSAGPPARP"
BASE COUNT         5565 a        13840 c        13357 g         5884 t
ORIGIN      
        1 tctgggtgga cgacgacgag gagtaccagc ggatcgtgga gttcctcgac caggtccagc
       61 cgtccctcgt ctcgaaggtg aagctgtacc gcaagtcgac gccgctcttc gaggagtacg
      121 gcgtcgagag cgagatcgag aacgcgctca agtcgaaggt ctggctcaag tccggcggct
      181 acatcgtgat caaccagacc gaggcgctcg tcgcgatcga cgtgaacacg ggcaagttcg
      241 tcgggaagaa gaggctcgag gaaaccgtcg tgaagacgaa cctcgaggcc gtccaggaga
      301 tcgtccggca gatccgcctc cgcgacctcg gcggcctcct cgtcgtggac ttcatcgaca
      361 tggaggaaga agggcaccgg caggagctct tcacggttct cgagaccgag ctgaagaagg
      421 accgctcgaa gaaccggatg ctgccggtgt ccgaattcgg cctcgtcgag atcacgcgca
      481 agcgccagcg gcagagcctc gaacgcgccc tcacgatgcc ctgcccctac tgcgcgggct
      541 ccggccgcac gaagagcgtg ccgacgatcg tcctcgagat ccggcgcgag atcgccaagc
      601 gcgtcgagga gggcgaggcg cactccctca tgctccgcgt ccatcccgag gtctaccggt
      661 cgctcgtcgg ggaggagcgc cggctcgtcg aggagctcga ggagcggatc ggcatgccga
      721 tccacgtcca gagcgacgag cacctccacc acgaacgttt cgacatcgtt ctggaggcct
      781 agagggctcc cgggaggccg attcgcgccg tttcggcgcg aatcggctgg cacggggcgt
      841 gcgatagcgc ggttgcgcac atccaacctc ctgccaaggc cgccccacgg gcggcctctt
      901 tttttgtccc gccggctctc cccctagact ccgcccgtga ccgccgaacg cctcttcacg
      961 cgcaagttct tcctgatgtg cgggttcacg ttcacggtct tcctgtcgct cttccagctc
     1021 ctcccgacgg cgccgttccg gatcctcgag ctcggcgggt ccacgaccgc cgccggcctc
     1081 ttcctcggcc ttctcacgta cgcctcggcg ctctcggcac cgctcacggg cgccctcgcg
     1141 gaccggatcg ggcggcgccg gatgctcctc gtctcgagcg tcgcgatcgc cttcttcgcg
     1201 ctcgcctacg cgctggcgcc gcgctgggag gtcctcgtct ttctggtttt cttccacggc
     1261 ctcttctggt cgggcctcct ctcggcgtcc gccgcgtaca tgacgggcct gatcccgaac
     1321 gagcggcgcg ccgagggaat cggctactgg ggaatggcga ccgtgctcgc gaccgcgttc
     1381 gcgccggccc tcggcctcgc cgtctacggg ctcggctgga cgtcgctctg cgcctcggtc
     1441 ggcgtcctga atctcgcgat ggccgcgatc gcgtggaacc tcgagccgga tccgccgcgc
     1501 gcagcgccgc acgggccgtt cttcacgcgg gacctcgtgg agtggcgggt cctcgccccg
     1561 tcgctcgcgc tcttcctctg cagcttcggt tacggaggcg tgacgagctt cgtcgcgatg
     1621 tggacggaga agaacggcct cgcgccgcgc ggcctcttct tcaccgtctt ctcgctcacc
     1681 gtgctcgtcc tcaggccgtt cctcgggcgg ttcgccgacc gcgtcgggcc gcggcgcgtg
     1741 atgtttcctc tcctcggcac cacggcggcg gcgttcgcgc tcctcgccgt cgcgacgacg
     1801 cggccgctcc tcgcgctcgc ggcgttcctc ttcggcgccg ggttcggcaa cgtctacccg
     1861 gtcttcgccg cccacgtgac gcggcacgtc ctcccggagc ggcgcggcgc cgcgttcggg
     1921 gcgatcctcg cggcgttcga cgtcggcatc gggacgggtt cgatcgcgct cggcgccgtc
     1981 atcgggcgcg cgggcttttc cgcggcctac gggacggccg ccgtcctcgc ggccctgtcc
     2041 gtgcccgcgt tcctcctgct ggaccggcgt gctttccccc ccggagccgc gctatcctcc
     2101 acgccatgaa taccgctccg cgcctctccc gcttcgcctg gaaggagttc ctccgcgggc
     2161 cgctcgggac gccgatcgtc ctcgcgcgcg gcatccgtcc cgtcggccgg ctgatgccga
     2221 tccgcacgtc ggcgctccgg acgtggaggc gcgtctcggc tctccccctg cccgcgagca
     2281 tccgcaaggc gctctaccgc gccgtctacg tcctgccgct cgggcccggc gatcccgcga
     2341 cgctcacgcg cgtcgtcgcg cggcaccggg cgttcttcgc cgcgcgcgcc tgaggcgcgg
     2401 ctctccgcag cgccccgccc cctcagtgcg ccggcgtccc cgcgcttggc acggcgccgt
     2461 cccggagaac gaagtgcccg ccgcggaagc tcgcgccgcg cagcgtcact gcgagcgcct
     2521 cgccgagccc gtccaccggg tgcacctcga tcgcgtcccg gacttccttc gggaggtccg
     2581 cgaggtccgg ctcgttgtcc ttcggcagga cgatcgtccg gatgcccgcg cgcacggcgc
     2641 cgaggacctt ctccttcagg ccgccgatcg ggaggacgcg gcccgagagc gtgatctcgc
     2701 cggtcatcgc gatgtcgtgg cgcgcgggca cccgtgagag cgccgacacg atggccgtcg
     2761 ccatcgcgat gcccgcggag ggcccgtcct tcgggatcgc gccggcgggc acgtggatgt
     2821 ggaggtcgcg ctcgaaggcc tcgtcgggga tcccgagctc ggccgcgtgc gagcgcgcgt
     2881 acgtccacgc ggcgcgcgcc gactccttca tgacgtcgcc gagctgcccc gtgaggacga
     2941 cctcgccctt gcccttcatg agcatcgcct cgatgaacat gatgtcgccg cccgcgggcg
     3001 tgtagtacat ccccgtcgcc agcccgacct ggtccgtgac gatctttttc tcggggtgga
     3061 cccgcttctt gcccaggagc ggaaagacgt ccccggcctc gaccgcgacc gccgcgacgt
     3121 cgcccgccgc gatccggcgc gcgaccttgc gcgcgagctt gccgatctcg cgctcgagct
     3181 ggcggacgcc cgcctcgcgc gtgtagctcg tgacgatctc ggcgatcgcg gcgtcggtga
     3241 gcaccaactg cgcggcggtc aggccgttct cggcgagctg ccggggcagg aggtactgcc
     3301 gtgcgatcgc gagcttctcg cgctccgtgt agcccgagaa ctcgacgact tcgagacggt
     3361 cgaggagcgg cccggggatg ttctgaatga aattcgcggt cgcgatgaag aggacctcgg
     3421 agaggtcgaa cgggacgccg aggtagtggt ccgtgaacga gtcgttctgg gccgggtcga
     3481 gcacctcgag aagggcgctc gcggggtcgc cctggaacga cacgccgagc ttgtcgagct
     3541 cgtcgaggag gaacaccggg ttcttcgtgc ccgcctgctt gaggccctgc acgatgcggc
     3601 ccggcatcgc gccgacgtac gtccgccggt ggccgcggat gtccgcctcg tcgcgcgcgc
     3661 cgccgagcga gatccggacg tacttccgcc ccatcgcccg cgcgatggac ttcgcgatgg
     3721 aggtcttgcc gacgccggga gggccgacga agaggaggat gcggcccttg cccgtcgaca
     3781 tgtccttctt cagcgcgttc tcggcgccct cggccgcctg ggcctcggtc ttctcggcct
     3841 tcgcgcgcat ctggcgcacg gcgaggaact ccaggatccg gtccttcacg tcgccgagcg
     3901 cgtagtggtc ctcgtcgagg atgcgcgcgg cctcgttgac gtcgagcttg tcctcgctgc
     3961 ggacgcccca gggcagctcc gccacgttct cgaggaacgt ccggatgacc tgcgactcca
     4021 tcgactcgcg tccgatgcgc tcgaggcggg cgagctcgcg gtcgacttcc ttgcgcacct
     4081 cctcggtgag cgggtgcgct gcgagcttct tcttcagctc ctcgaggtcg gcggcgtcct
     4141 tgccctcgcc ctcgccgagc tccttctgga tcgcccggag ctgctcgcgc aggagcatct
     4201 cccgctggcg gtcgccgatc tgctccttca ccttcgactg gatgtcctcc tgcgcggaaa
     4261 ggacgccgat ctgccgctgc acggcgacga gcacgcggcg caggcggtcc tcgaccgaga
     4321 gcgtctcgag aagctgctgg cggtcggcca tggggatgtc gatgtacccc gcgacgaggt
     4381 ccgcgaggcg gcccggctct tcggcctgct cgaggaactg cgcgaccgct tcttccggaa
     4441 ggcccatctt ctcgccgagc tcggcggcgc gctcgcgcgc ctccttgtgg agcgcgagga
     4501 agaccgggtc cttcgcgtcg gccggcgcca tctcggccgc gggcacgacg acggcctcga
     4561 gcatcccgcc cttcttctcc gtcacgcgca tcgcgattcc gcggcgctgc ccctcgagga
     4621 ggaggcgcat gccgccgagg ccgcgctgga ccgaactcac tttcgcgatc gtcccgatcg
     4681 tgtagaggcc ctcgggggtc accttctcga cgtcctcgcg ctgcgagagc gcgaagacga
     4741 gcgcgtcccc gcgcagcgcg gcctcgatcg cccgcaccgt gccgccgcgc ccggcggaga
     4801 tcggcgtcga cacgcccggg aagaggacgg cgttgcgaag ggggagaacg ggcagggtca
     4861 gggtttccgt catgagcacc tcaacgccgc gcattcgccg actattcccc aatgtgagtc
     4921 ggctccgcgc agatcgccag aagagaattt cttagagtgg tttaggagtc ggtccttctt
     4981 cagcggccga gccgccgcgc gaggatgtcg acgccccgca gcgcggcgtc cggcgggacg
     5041 gccgtgtagc agaggcggac gtgcgtcggg tacgggccga agctggggcc gggggcgacg
     5101 aagagaccct cccccgcgac gtcttcgaga agccccagga ggccgcgctc gtcgagcgcc
     5161 gcggccacgt cgaggaagag gaacatcccg ccctcgggag cgggctgaga gagccggcgc
     5221 gcggcctcgc gccccgtcgc cgcgtactcg gcgcgcgcct cggcgaccca ggcgtcgccc
     5281 gcacccgcga ggatgcggag cgccgcgtgc tgcgacgccg tgggcgccga gtagatcgtg
     5341 tgcgccccga ccttcacgat ctccgcgagc gcggcctccg gcccgacgag ccagccgcag
     5401 cgcgccccgg agaggcccca tgccttggag agcgagtgtg ccgcgatcgt ccgctcgggc
     5461 gccgccgcgt acgcgggccg gtgggcgccg ccgtacgcgt agagctcgta gacctcgtcc
     5521 gcgaggagcc agagattccc ctcgcgcgcg acgtgcgcga gggcgtcgac ggagccgggc
     5581 ggcaggaccc ggcccgtcgg gttgctcggc gtgttgacgt agatcgcggc cgtcctctcc
     5641 gtgacgcgta cgcgaacggc ggcggcgacg gcttcgggcg acaggtcgcc caggaagagc
     5701 ggcacctcga ccgggaccgc gcccgcgagc cggacgtgcc cctcgatgag cggccagcgc
     5761 gggccgagga ggaggacctc ttcctccggc gcgacgagcg cggcaaccgc cgccatgaga
     5821 cccgcggtcg caccgcccgt cacgaggacg tttgcgcgcg cgaccggcgc tcccgtctgc
     5881 gcggacgcgc gcgtcgcgat cgcgtcccgc agttcgggca gcccccagac gtccgtgtag
     5941 cggttgatcc cgggcaggcc gggcccgagc tcctccggcc ggcagcccgc gggggccttc
     6001 cgccacgtat ccccgacgtg gagggggaag acctccccct cgtgcgtcgc caggcgcgcg
     6061 aggaggctcg aatacgggtt cccgtggagc gcggcgacgg cgggagaggt gcggggcggg
     6121 cggggcatcg gtccatccta gcccggcgcc tgaaaccctt cgcgcccgga tccgtccttc
     6181 tctccgccat gggcgcgctc gccgacctcc cggcctacct ccgccgcatc ggtctcggcg
     6241 cgcctcctcc cgcgaacgcc gaaggcctcg ccgcgcttca ccgcgcgcac gccggcgcga
     6301 tccctttcga gaacctcgac atccttctcg ggcgcgagat ccgcctcgac ctcccgtcgc
     6361 tcgaggccaa gctcgttgcg gcgcggcgcg gcggctactg cttcgagcag aacaccctgt
     6421 ttgccgccgt cctcgccgac gccggctttc gggtgacgcc cctcgcggcg cgggtgaggc
     6481 tcggcgggcg cacggacggg ccgcgcacgc acatgctgct cgccgtgcgc gccgagggcc
     6541 gcgactggct ctgcgacgtg ggcttcggcg gcggcgggcc gtgggaaccg ctgccgctcg
     6601 agccttgcgg cgagatcgcc caggggctct ggcgcttccg gctcgtcgag gacggcggcg
     6661 aacgcgtcct ccagaaccgg gacccgtccg gctggcggga cctctacgga ttcacgctcg
     6721 aaccgcagcg gccggcggac tacgccgtcg caaaccacta cacgtcgacg catcgggatt
     6781 cgtcgtttac gaaggtcgcc gtcgcgcagc gcacgtcggc gactggcgcc ctcgtgctgc
     6841 gcggcgacgt tctccagacg atgcgccccg gcgaggcgcc ggccgagacg ccggcgccgg
     6901 agggcgacgc gctcctcgac ctcctccgcg agcgcttcgg gctcgacttt ccagcgggca
     6961 cgcggttccg gccgctctga gcgcggcgcc cgttgcggca caatcgtccc cccggggtcg
     7021 tacgcccggc ccgaggaggt cccgtggtcc gaaccgatcc ccatccgccc gaagttttcg
     7081 agctcctgcg ccggaagacg gctgcgacgc gcatcgccgt cgtcggcgcg agcaacgtcc
     7141 cctacaagta cggcaacgtg atcgtgaaga acctcgccgc caagggctac acagtcctgc
     7201 ccgtgaaccc gagggagaag gagatcgcgg gcctgcccgc gtaccccgat ctcgcgtcgg
     7261 tcccggggcc cgtgcacatc gtcgacgtcg tcacgccgcc ggcggtcacg cacggcatcc
     7321 ttcgggaggc tgcacgcctc gggcttccgg ccgtgtggct ccaggacggg agctatgacg
     7381 acgaggcgct cgccatcgca gccgcggcgc cctacaagac ggtgtacgag gcctgcatca
     7441 tggtggccag caacttcggc tgacccctcc cgcgcggggc tccggccccc tatgatcccg
     7501 tcttcacccc gtttccctcg tccatggtca cgcgcccctc ccggaggttc tcgtgaagaa
     7561 gaagacgttt gccgccgcgc tgcttctgag tctcgtttca cgccccgctc ccgcactgga
     7621 caaggttcgc gtcgagactc gagtcgacgc cgcgttcgcg aagtacggcc tcacgggccg
     7681 gggcgtgatc gtcgccctcc tcgaccgcgg gatcgactgg aagaacaacg acttccggaa
     7741 cgcggacggg acgacacgca tcgcctacat gctcgacttc acggacaaca ccggcgcggc
     7801 cacgagcggg tacggccggg ggacgctcta cacgcgcgcc cagatcgacg ccgcgctctc
     7861 cggcgggccg acgatcccgt tccgggacgc cgtcggccac ggcactccga cggccgggac
     7921 gccgaccggc aacgggcgca acgtcgcgaa gtaccgtggc atcgcgccgg aggccacgat
     7981 catctccgtg aaggtcacgt ccgacggcgc gcccgcgcac gacggcgagg cggccgaggc
     8041 cgggttcaac gacgccacgg tgtttcctct ggcgatcgac ttcgtgaaag ccaaggcgca
     8101 ggagctcggg atgccggccg tcatgcttct caacctgggg agccagggcg gtccgacgga
     8161 cggcacgagc agcctcgcgc gcaagatcga cgcgaccgtc gggcccggca ttccgggcct
     8221 cgtcttcgtc tcggggccgg gcgacgaggg cggaagcgcg aaccgcgcgg gcggcaacgt
     8281 cgcgagcggc tcgacgcagt cgatcaggat ccacaagggc caggcgggcg gcctgctctt
     8341 cgatctctgg taccccggcg ccgaccgttt cgacgtcacg gtccagaccc cgacggcgac
     8401 gtacgggccc tacgtctccc cggcgacgaa cgcggattcc gcgacggtca cgaactccga
     8461 gttcacctac taccagcaag gctcgagccg cgtcttctac ggcgcccaga acggcaagcg
     8521 cgagatcttc ctgaacatct cgggcccggt cggcgactac acgatcacgc tgaagggaac
     8581 ggccgtgacg acgggccgct tcgacgcgac gatcaacccc tccggtcacg ggggatcgaa
     8641 ctacttcctc gaccacgtcg cgcccggaag catctgggac ggcgcgacgg cacgctacaa
     8701 catctgtccc ggcgactacg tcatccggac gagctacgtg gacatcgacg ggatcacgcg
     8761 aacgatcacg ggcaacggca gcgtcggcga gatctggccc gggagcagca tcggtccgac
     8821 gttcgacggc cgtctcggca tcgacgtcgt ggctccgggc gacagcctct tcagcgtcta
     8881 caacccgaag tcgtactggg ccacctcgcg cttcaacctg atccaggacg gcctcggctt
     8941 ctacggccgc gcgaacgccg tgagcgcggc ggccccgatc gtcacgggga tcatcgccct
     9001 catgctccag gcgaacccga cgctcgacgc gccgaccgtg aagtccatcc tccagcggac
     9061 ggcccgcggc gactcgttca cgggcgcggt cccgaacacg acgtgggggt acgggaaggt
     9121 ggacgctctc gcggcgctcg acgccgtgaa tacgacgacg ctcttcgtcc cgatcgcgct
     9181 ctcctcctcg ggcgcgaacg ggtccttcta cacgacggag ctggcgctca cgaaccgcgg
     9241 gacgacggac gcgacgatca catacgccta cacggccgcg ttcggcgggg cgagcggcgc
     9301 cgcgacggag acgctcgggg cgggccggca gaagacgtac gccgacacga tcacgagcct
     9361 ccgcgccctc gggatcccgc tcggcgacgc gggaaaccgc ggcgggacgc ttcaggtcac
     9421 gttcggcggc ctctcgtccc cgtccgcggc cgcggcgacc gtccgcacgg cgacacccgt
     9481 cgccgacggg cgggccggcc tcgcgtacgc cggcttgccg tcgtcgcgcc tcctgtccac
     9541 ccccgtctgg ctctgcggcc tcaggcagaa cgcgacggac cgttcgaacg tcgcggtcca
     9601 gcacgcgggc ggcccctcgg acggcaacgt gacgctgcgc ctcacggtcg tgtcgggcga
     9661 cccggcgaac ccgggctcgc tcgcgctgcc ggacgtcacg ctgtctccgg gcggtttcag
     9721 ccagatctcc gggatcctcg catcgaacgg gctgagcttc tccaacgggt tcgtgaaggt
     9781 cgagcgcgtc tcgggcaccg cgcccttcta tgcgtacggc gtcatcaacg atcaggcgaa
     9841 ctccgacggc tcgttcgtcg agcccgtgcc cgcgagtccc gcgtcggcga tcgggggcgt
     9901 cacgctgccc gtcgtcgtcg agacgagcac gttctcgacc gagctcgttc tgacgaactt
     9961 ctcggggagt tcccggaccc tacagtgcac gtacgtcgcc gcgccgctgc cgggcgggca
    10021 ggtgacgttc aatctccctc tcctcccgaa cgagcagcag atcctcccgg ccttcgtgca
    10081 ggttctgcgc tcgcgcggcg tcgtccccgg cgggcccggc gcgacgtttg cgggagcgct
    10141 cttcgtgcgg gatgcttcgg gcgacctccg cggcgtgtcg atcggggcgc ggacgtcgtc
    10201 cgccggcggc ggcgggcgtt acgggctttt ctactccgcg gtcccgaacg gcgccgaggc
    10261 gacgaccgtc gcgtggctgt acggcctcca gcagaacgcg gacaaccgca cgaacctcgc
    10321 gctcgtgaac gtcgggtcca ccgacgcctc gacggacacg tttcacatcg acctctacga
    10381 cggcgcgacg ggcctgaagg ccggcgagac cgacgcgacg gtggccgcaa agggcttctt
    10441 ccagatcggg acgatcctcg cggcgtacgc gccgggcgtc acgagcggct acgcgaaggt
    10501 cacgcgcacg tccggcaaca acccgttcgt cgcgtacgcg gtcatcaacg acggcggcca
    10561 gccgaaccag aggagcggcg acggcgcgtt cgtgtcggcg gtggtggggc cgtgaccgcc
    10621 gcccgcctca ggcccgaagc gccgccgtga tcctcgcgat gagcgcttct tcctcggccg
    10681 agatggcgtt cgcgcgcccg aacacgcggc caagcccccc tcccgacgcc tcggccaccc
    10741 gccggaagga atcggcgtac gcgtcgaccc gctgcttttt ctcctcttcg ggaagaaccg
    10801 aaatcccgac cttgaggacc tcgaccgccg cgtcgtagag cgccgccgac ggctgcttct
    10861 tcagccattc gaggacctgg gtgtgggcgg gcgagccggg cccggttccg cgcgcggccg
    10921 ccatgccgag tatggtctcc cgctcgcgtt cggtcacgtc gccgtcggcc cacgcgatct
    10981 ggacgagcgg agccaggaac agggccgggc ccgtgtcgtg cgtgatgccg agatcgatga
    11041 tccgctgccg cagcgcggga tcgtccacgc gcagctttcg cgcgagggcg gcggagactt
    11101 cctcgagctt cgcgcgctcg cgcagcttca tgaggagctc ctcgtcccgc tgccgggccc
    11161 actgggcttc catcgcctgg ccacgctctc gaaggatgtc gcttgccatc ggccctccct
    11221 cccgaaccta ccctcaaagc ccgaatgggc gcaacgccgg cgtcgttaga attggtgcgc
    11281 ccgaggcgat tcgaacgcct ggccttcaga tccggagtct gacgctctat ccagctgagc
    11341 tacgggcgca tccggggcgg gaaaaactgg ggtggaagac gggggtcgaa cccgcgacat
    11401 tcggaaccac aatccgacgc tctgccgact gagctactcc caccgcactc gatcggaccg
    11461 cggaggatag cagatcgggc ccccgcgtga gccgccccgg ccgccgttcc cgtaacatcg
    11521 gcggttaccc catggacgcg ctcgacgaac tgagacggcg gatcgacgaa acggaccggc
    11581 agctgctgga gacgctcgcg ctgcgcctga agttcgtcgc cgaggtggga cgcctcaaga
    11641 gcaagggagt tccgttcctg cgcgaccacg cccgcgagag cgccctcctc gcgaaagtcg
    11701 aggcgcaggc gaaggaactc ggcctcgacg cgttccgcac gaccgagatc ttccgcgagg
    11761 tcatcgcgat gtccgtgaag gcccaggaag aagcccttct cgaccgcaag gccgccgagc
    11821 gctcgctcgg gaatgctcac cgcgtcgcct tccagggcgc cgagggcgcc tacagccaca
    11881 tcgcggcgcg caagtacttc ggcgcctctg cgacggagtt cgcgggattc ccgacgttcg
    11941 cggcggcgct cgccgaggcc gagcgcggcg acgccggcta cgcgttcctt ccgatcgaga
    12001 acacgaccgc gggctcgatc aacgagacgt acgacctcat ccgccacacg gacctgaaga
    12061 tcgtgggcga ggagatcctg gaggtgcgcc actgcctcct cgcgctgccc ggtgcgacgc
    12121 tcgagggcat ccgccgcgtc gcctcgcacc cgcaggccct cgcgcagtgt gggcgctttc
    12181 tcgcgcagct ggccggcgcg tctgccgttc cgtacatcga cacggccgag gccgcccggt
    12241 tcgtgaagga gtccggcgac cgctcgctcg ccgcgatcgc ttccgaggag gcgggcgctc
    12301 tccacggcct cgcgatcctc gcgcgcgacc tcgcggacca ggaggagaac tggacacggt
    12361 tcgtcgtcgt ctccgcgatc ccgatcgtgc tcgacccgcg tatcccggcc aagacgtcgc
    12421 tcgtcttctc gacgccgcac cggcacggcg cgctcgcgca ctgcctgaac gtcctcgacg
    12481 cgcacggcct gaacctcacg aaactcgagt cgcgacccgt gccgcgccac ccgtgggaat
    12541 acctcttcta cgtggacttc gagggcgccg ctggcgagga tgcggcttcg cgcgcggtcg
    12601 cggcgctgtc cgcagagtgc ccgtacctga aggtcctcgg ctcgtacccc gcacggacca
    12661 cgaagaaggg gcgggtcgac cgcctcccgg aggccccccc gtgatcgtcc tcgtcgacga
    12721 gaccaccgcg aagaacgccg ccgccgagaa ggagctcctg gacgccctcg gccggctgcc
    12781 cggcatcgag gtccgcgtcc acaaggtccg cggcgccgag aagaccgtca cggaggtcta
    12841 cctgatcggc gacacgcacc ggctcgccgt agaggacgtc gccgttctcc ccggcgtcga
    12901 gcgcgtcgtc cgcgtctcgc aggaataccg cgtcatcggg cgccacaagg acgaccggcg
    12961 cgcgagcggc ttcacgtaca acggcgtcga gttctcgcag gacaacctcc acgtcttcgc
    13021 cgggctctgc gccgtcgaca cgccggagaa cgccgacgcg atgttcgcgg cgctcgcgtc
    13081 gttcgggcag accacgacgc gcatgggcgc gtacaagccg cggacgaacc cgtacgcgtt
    13141 ccagggccac ggaaaaggct gcctcccgtg ggtcttcgag aaggcgggcc tgcacggcat
    13201 ccgcgtcatc gccatggaga tcacgcacga gagccacatc gacgagatcg ccgagtgcct
    13261 cgagaagacg ggccagccga cgggcgtcat gctccagatc ggcacgcgca acatccagaa
    13321 cttcgagctc ctgaagacgg tcggccggca gaccgtgttt cccgtcctcg tgaagcgcgg
    13381 cttcgggatc acgctcaacg agtcgctgaa cgccgccgag tacctcgcct cggagggcaa
    13441 ctcgcagatc gtcttcggcc tgcgcggcgt caagacgaac ctcggcgacc cgcaccgcaa
    13501 cctcgtggac ttcgcgcacg ttcccgtcgt caagcggctc acgcgcctcc ccgtgtgcat
    13561 cgacccgtcg cactccgtcg ggagccgcga gaaggcgccg gacgggatcc cggacctcat
    13621 gcacgtcacg gcgcagggcg tcgcggccgg cgcgaacatg gtcctcgtgg acttccaccc
    13681 gcaccccgcc cacgccctcg tggacgggcc gcaggcgctc ctcctctcgg agctcggctg
    13741 gttcctcgag gacgtggccg tcgcacgggc ggcctacgag aagcgacgca cgctcgcgct
    13801 ctcgcacgcc gcgggcgcac ccgtctccgc cgcgacggca tgaccgcggt cttccgcgag
    13861 gcggcgcgcg tccgggcgag cgaaatcggc ccggccggga cgatgcgtct gcccgcgctc
    13921 ctcgacctct tccaggaggc cgcgggaaac agcgcgacgt cgctcggctt cgcgtcggac
    13981 acgcttctcg ccgaagggaa aacgtgggtc ctctcccgtt tccacctgcg gctcctcgcg
    14041 ctgcccgcgt ggcgggacga gatcgtggtc gagacgtggc cctcgggcgt ggcgcgtgtg
    14101 ttcgcgctgc gcgagttccg cgcgacggga acggacggcc ggctcctcgc cgaggccacg
    14161 tccgcgtggc tcctcgtcgc ggtgccgtcc aagaaaccgc tccgcccgcc ggcggaaatc
    14221 gccgcggccg cggccggcac cccgccgcgc gtgatcggcg acgcgttcga gccgctcgcc
    14281 gggccggggc ccgacgccgc aaaagggccg cagacgcgca tcggccgctt cgacctcgac
    14341 ctcaacgccc acgcgaacaa cgtcgcgatc ttccgcgcgc tcctcgaggc gctgccgcac
    14401 gcggacccgc ccttcgatct cgaggtcgag ttcagagggg aagcgttcga gggggatttc
    14461 ttagaaggga gaaccgacga aggcggaggt gcgcgacgcg tagcgctcgt tcggccggcc
    14521 gacggccgtg agatcgcgcg cgccgtcctc accgtgcgcg gctgaaaagc cccgcgaagt
    14581 ccttttcgac ctcgtccacg gccgcgagga gctgcggcgt cggggcgtcg tccgactcct
    14641 ggaccatccc gtagaggcgc gtgagcttcc cctcgaggga ctcggccttc ccggcgtccg
    14701 cgcccttctt ccgcgcttcg gcgaggcgcg cgaggtccgc ggcgaggcgt ttcgcggccg
    14761 catgctgcgc cgcgaggtcc cccggggagg ccttgacgcg cgggtccatc ccgaccgtca
    14821 gcgtccgcgt gagcgtcctc ccgtccaccg tgagcgcggc ggtgtacgtg ccgggcagaa
    14881 cgagcgggcc ctccggctcc ggcggcgtgc gagagggaac cgcggagatc gggtactcgc
    14941 gcgatgagac cggcggcggc gcgtaccgga ggtcccacac ccagcggtgg tgtcccgcgc
    15001 tccggggcag cgtgagcgca ggccgcgccc agtccgccgt gacgacgagc ggctcgacct
    15061 tcgcctcggg cgcgtcgtcg ctcgcgtacc ggcgcacgac gccgccggcg gcgtcccgga
    15121 tctcgagcac gacgggggtg gcggacgaaa cgccgaggac gtagccgagg atcgcgccgt
    15181 cgggcgggtt ctcgccggcg ggctcctcga tgggcacggg cgtgtccgtc cagcggctcc
    15241 tgcggatgcg cgtcgccttc tgcggctcga agaggtgcgc ccgcgccgcg gcgacggccg
    15301 gcgtcaactg ccggagcggc gtcacgtcgt cgaggatcca gaacgagcgg ccgtgcgtcc
    15361 cgaccacgag gtcgtcgccc ttgacgacga ggtcgcgcac cgacgtcgcg ggcatgttca
    15421 gccgcagcgg ctgccagttc gcgccgtcgt cgaacgaaac gcacaccatg cgctccgtcg
    15481 ccgcgtagag cagccccttc ctcacggggt cttccctgac ggcgttgacg gggtcgtcgg
    15541 ggaggccgtg cacgacctcc tcccacgtct tcccgccgtc gtgggtccga aggacgtgcg
    15601 gcttcatgtc gtcgaggcgg atgcggttga tcgcggccca ggccgtgagg tcgtcgtgcg
    15661 gcgaggccgc gagcatggac accttgctcc acgccgtgag cccgggaggc gtgacgtcgg
    15721 cccacgtctt cccgccgtcg gcggtgcggt ggatgaggcc gtcgtccgtc cccgcccaga
    15781 gcacgtcggc gctcttgtac gccggcgcga gcgtgtagac gacgccgcgg cgcttcatcg
    15841 tctcgaggtc cttcgtccgg aagacgccga tcgaggccgg gacggccgtc ctctcgcgcg
    15901 agaggtcggg gctgatcacg tcccagtggc ggccgccgtc cgccgtccgg aacacgacgt
    15961 tccccgcgta gaagaggagg cgcgggtcgg ccggcgagaa gatcaccggc gccgtgcgca
    16021 agaagcggta cgtgccgcgc aggggcgcgg gcgagacgtc ctggacctcc ttggtcgagt
    16081 ggcggtagcg cgtgagcttg cccccgtaga cgacgtcggg gtccttcggg tccggcgcga
    16141 cgtagccgta ctcctcggcc gcgacgggat gccagtcgcg ggacgtgatc gcaccgtcgt
    16201 cgccgcggct cgcgatcccc accgagccgc tctcctgctg cccgccgtag acccagtacg
    16261 ggaagcggtc gtccgtcgac acgtggtaga gctgcgcggt cggctggttg taccagctgc
    16321 tccacgtggc gccgccgttc accgagacga tcgcgccctg gtccgccgcg agaaggatca
    16381 cgtcggggtt ttccggatgg aaccagatcg tgtggaagtc gtcgccgccc ggggcgccgc
    16441 gcaccggctc gaacgtcttt ccgccgtcct tcgaccggta cgcggacgtg ttcgcgaccc
    16501 acacgacgtc cttcgagccc gggtggacct tgatctcggc gaagtccgag ccgcggcccc
    16561 agagacgctt ctcgccgctc accttcacga acgtctcgcc cgcgtcgtcc gagacgaaga
    16621 tcccgccgcc cgactccttc tcctcgtcga gagcgtcgac ggtcgcgtag acgcgcttcg
    16681 gttccgacgg cgcgatcccg aatccgatgc ggccgccgcc ctgggcgtgc gcgggcaggc
    16741 ctttgccgat cgcccgccac gtcgtcccgc cgtccgtgga cttgaagagg cccgtgcccg
    16801 gcccctgcca ctgcccgttc tcccacggcc cgagccgcga ggaccagagc gccgcgtaca
    16861 cgacgttcgg gtccttcggg tcgagcgcga ccgccgcgcc gccggtgtcc gcgtccctgt
    16921 agaggacctt ctcccaagtc ttacccgcgt ccctcgtcct gaagatcccg cgggtctcgt
    16981 tcgcgccgta cgggtggccg aggaccgcga cgaacgcgcg gtcggcgtcc ttcgggtcgg
    17041 cggcgatcgc cgcgatctgc tggccgtccg cgagaccgag gcggacccac gatttcccgc
    17101 cgtcgtccga acggtacatg ccgttcccca cggagaggtc cgggcgctgc aggccctctc
    17161 cgctccccac ccacacgcgg tcggggcgcg accacgagac cgcgagcgcg ccgatcgagc
    17221 ccgagggctg cgcgtcgaag atcgggaccc acgtgcgccc ggcatccgtc gtcttccaga
    17281 cgccgccgtt gttcggcgcc atgtagaaga cgttcgtgtt tcccgggacg ccggtggcgg
    17341 cgaccgtgcg gccgccgcgg aagggcccga tcatccgcca gcgcatctcc ccgaacaggc
    17401 cctgggggaa gggctgcgcg acggccggga gcgcggcgag gaaggcgagc gcgagaaggc
    17461 gcgacggcgt gcggtcgtgc gtcatggtca cttcgcgggc ggcgtgaccg cctcgaggcc
    17521 gtggcggcgg ggtcccctct cgctccgcca gaggaggaac gagaagacga acccgacgga
    17581 ggcgagcacc ccgagcgtca cgacgacggg cgtccagcct tccgggcgtt cggcgctcgc
    17641 gtgcgaggcg tccttgagga ggccgaggcc ccagctcatc ccggcccagc ccacctgctg
    17701 gcagaacgtc atgagcgcgt atgccgagcc gagccgggcc tcggggacga ggtacgtgac
    17761 ggcgggccag aggaccgccg ggacgagggc gaacgcgagg ccgagcattc ccatgagaag
    17821 cgtcggggaa actccggtga agggcaacag gaagaagggc gggacgagga gcgccgagcc
    17881 caccgccatc aggagcgccc gcttcccgat ccggtcggcg agcaggccga agagcggcat
    17941 gccgatcatc gacaggagcg ggaggatcga cttcaggttc ccggcggcct cgggcgacag
    18001 gcccttggcc tcgatgaagt acaggttcgc gaacgtccgg aacgggaaga tcgtcgcgta
    18061 gaaggcgacg cagaggccga cgacccacca gtagccgagg tcgaacctca cgaggtccga
    18121 ccacacgagc ttgtccgtca tgccgggcct cgacaccgcg tagacacgcg cggcccggcg
    18181 ctcgagcccc gagtagacga tcgcgaagac gagccacacg acgccgagag cggccgcgag
    18241 gagcagcggc ggctgccagg tcgcgaagag gggcttgaag aggtcgggcg acttgtccgc
    18301 ggcccaggag ccgagacgcg cgacgaggag ctggatcgcg agcgcgaacg agatctcctt
    18361 gcccttgaac cagcgcccga tcacggtcgt cgccgcgacg atgaaaagct ccgagccgat
    18421 cccgaggagg aagcgcccgg ccatcatccc ggcccacggc gcgccgggca cgaggaccgg
    18481 aaggaccgcg atcaggatgc cccccgcggc gccgatggcg ccgaagagaa tggccgaggc
    18541 cgcgacgccg atccggtcga tcaggacgcc gcccgcgatg agcgtcagga gcgccgcgac
    18601 gttgtaggcc gtgtcgagga gtccgatctg ggctcccgtg aagccgaagg ccttttcgag
    18661 gagcggcgtc accgggtaca gcgcgtcgaa gacgtagtac accccgaaca tcgcgaggct
    18721 gcacgtgaga agaaccgccc accggtaggc gggcggcggc ggcgcgaggg cggagcccgg
    18781 ggcggaactg tccggcatcc gtgcagtcta gcctctccgc gcgggtgccg ggccgcctcc
    18841 acgcgcccgg ggaaggttca cggaatcatg tcgaatcgct cgacggagcg cgccgggcgg
    18901 tcgacggtca ccctcacgac gagaccggcg tgatcggacg gccagaggcc ggccggggcg
    18961 acgtcgccgc gtgtgttccc gacgacgttg gcgctcacga gatcgaagcc gtctcccttg
    19021 aggaacacga agtcgatccg ctgcgtgaaa gccgggaacg gggcgaggag atccgggttc
    19081 gggccccacg tcaggccggc gtccttcggg tgcagcgcga gccacgcgtc cttcagtccg
    19141 ggtccggccg gcgcgggcgc cgtcaggaac cgataggtcg gccagcccgt cgcggcgtcc
    19201 gtgttgaagt cgcccgcgag cacgaccctc aggtcggttg cgaacggccc tgtctcgccg
    19261 agcgtgagct cggccgcctg cgcgagctgg agctgctcga gaggcgaggg ctcctcgttc
    19321 gcgacgggcg ccggcacatc ctcgaggtgc gcgacgacga aacggaacct cgagccggtg
    19381 tcttcgacgt cgaccgaggc ccagccgcgc ttgatcacga gcgggacctc gcacgagccg
    19441 tccgccgcgg gcacgatgca gagctcggcg aggagggcga acgtcccgct cttcgggttc
    19501 gagacggaga ggcgcggctt tccgggagac ggctcgccgc gaacgaggat cgcgctccgg
    19561 tcgaggagca tcacgacgtg gccctccgcg tccgggagca tgacgttcgt gtcctcgacg
    19621 acggactgca cctcgtagaa catcccctcg gccgcgaggg cctcgacgag gatcgcgagg
    19681 tggtcgatca cctccatcgg ctcgaaggtc gtcccgtcga gacggacgta ctggacggcc
    19741 tcctggaccg cgaagaggtc gggcctggcg gccttcacct tcttcgcgat cgccgcggcg
    19801 cgcacgcgga agtcggccgc gtggacctga gcccacacgt cgctcaccgc ggcggcgatc
    19861 agctccgggt ctcccgacgc ggcggcaccg aagagcggcc cctcgtcgaa gccgaagtac
    19921 gtgttccagg tcatgacggt gatgggcgct ccgctggccg cggacgctgg ggggctgagt
    19981 gcgaggcccg cggcgatcgc gacggccgcg agcaggggga cgagacgcgc gtgcgagttc
    20041 atggggacct tcctttccgt ccgcgcgagt ccttgctcac gggacgaggg tcacgacgac
    20101 gccggcgtgg tcggacggcc acatgccggc cggaaggcgg tcgaggagcc tggcgccgac
    20161 gacgtcggcg tccgtcacgt cgaaccggcc cgagaggagg acgaggtcga tgcgctgcgt
    20221 gaggcgcggg aaggggttca ggaggtccgg cgactggccc cacgtgaggc cgggcgcgtt
    20281 gtggttcacg gctttccacg catccgtcag gtggagcgga ccggtcagga gcttgtacgt
    20341 gggccacttg cggaagccgt ccgtgttgaa gtcgcccgcg aagacgacgg gaaggccgtc
    20401 ggcggcgtga cggttcacga gctcgaccgc ctgcgcctcc tggatcggca gcaccgcggg
    20461 atccgcggac gcgtcctcga ggtgcgtcgt gacgaagcgg aagcgatgtc ccgaggcttc
    20521 gacgtcgacg gacgcccagc cctgcggcaa cgggaacggg aagccgactt ccggcccgag
    20581 gatgacctgg tagatgccgc tctgcgggtt cgagaccgcg accttggcgt ttctcgagcg
    20641 cgcgagaacg gcgacccgat cctggacgga gaccatcgtg agctccccgg ccgcgtcgag
    20701 gaccggcagt ccgatggcca gcgtgtcgac cgaggacacg acctcgtacg gaatgcccgc
    20761 gtcgtgcagc gccgcgacga ggatctcgag atggtcgagc tcctccacga tgcccgtcga
    20821 gccggcctgg gtgtacttga cggcttcctg gacgccgatg aggtccggcg cctcgaggcg
    20881 gacgcgcagc gcgatgtcgc ggacgcgcct gtggaagtcc gtcgcgtgga tctggcgcca
    20941 ggcctccgtg gccgccggca cgatgagcgc cggatcgccc gacatcgccg cggcgatcac
    21001 cggtccgtcg tcaaatccgt agaagaggtt ccacgtcatg accttgacgg gcttcggacc
    21061 gtccgccgag gcggccgggg cggccgcgac gcccagcgtc gccgccgcgg cagcgaagaa
    21121 agcgatcagt ctcgggcggc tcttcatggg gttcctgcct ttccgtccgt tgtgtcggac
    21181 gctccccaac gtaggcgcag cggaacccgc aatctcagcc gaatttcagg ggttttacgg
    21241 aacgcggccg gtcaggccgg gcgcgctccg gcgcggcccg gaccgagccg ctcgatgagc
    21301 gcctcgaggt agcgggactt cacctgctcc gaggcgaggc cgcgctggac gagcggcagc
    21361 ttcacgatcg cgccgaggac ggcgcccagg atgcggtggc tcgcgagcgt ccggtcgtcg
    21421 aagaggacct ccgcgagccg gccgcgctcg atcgccatga cgaccgtgcg gtggaccgtg
    21481 tcgagcggcg ggatcacgcg tttctcccgg cgcacgagac ggaggccccc cgtacggcat
    21541 gccgggacgc agacgccgca gcccaggcag atccgctcgt cgagccgcgc gaggcgcttc
    21601 ttcggaagct tcgggtcgtt cgcggagacg gagcccatcg cctccacggg gcaggcctcg
    21661 acgcacctgc cgcagcccgt gcagcgctcc gcgtcgacct ccgggacgaa gccggtcgtg
    21721 tggaccggct tgagaaaccc gaaccgccgc tgggcgatga gcgcctcgca gcagcacccg
    21781 cagcagttgc agatgaaggc gggctgccgg cgcacgttct cgccgaactg gacgaggccc
    21841 tgcccccggg cctgtgcgag caggtccagc gcctcggcgg aatccgactt gcgcgcgtgg
    21901 ccgtggcgca cgagcgccgc cgccgtgccg ccgaacgtca ggcagatctc catcggcgcg
    21961 ccgcacgccc ggccgagatg gctcatcttg tgccggcagt agcagaggcc gacccccacg
    22021 tcgcggctcg tccgcgcgac ctcgctcgcg cgctcccagt cgagaacctc caggggctcg
    22081 tcctcgggaa gcgcgtcctc gttcacgaag gcgcgcccga gctgcgtctc gccgcgcgcg
    22141 aagagctcgc gtgcgaagtc ctcttcgacg ttgaggtacc cgtagaagag ctccgcgagc
    22201 gtcttctgat cgatgtcgcc gcgcagccgc atcatcgaga actcgaagaa gcccgccatc
    22261 ggcggcggga ggacccagcg cgtcacgccg ccgtgcgtcg catcgaggag gagcgcgcgg
    22321 gacgcgagcg cgtcgagtgt gttctgcgcc tcagcgggcg tcgtcttcca gatgcgcgcg
    22381 gccgtctcga gcgtgaacgg cttgatcgga agcgccgaaa cgagactcgc ctcgcgctcc
    22441 gtgaagagga tctgcaggat ccggtcgagg agctgcgagg ggggcgcgcc ctgcgggaac
    22501 cggttcagcc ggtcgacgag gtcccggtat ccggtgcgcg ccgtcgcgtg ggccatcgac
    22561 gcctcgggag gcgggaacac ctccccctgt ctcaaacgtg tgcccgcagc cgcccgacgc
    22621 ccatgaagca gctgcttcaa tggcggcctg ccgggggccg aacgcacctt gtaagaggag
    22681 ggatctcccc atgaggaaag ctccggtcgc cgtggcgctc gcgatcctcc tcgccgcggc
    22741 cctgcccctc accggcgccg gccgtgattg cagcaagctc accgaaatcc agatccaggc
    22801 gaagtccgac aagacgatct acaccggcac ctctcccgcg acctaccgcg tctgcgtgtg
    22861 cgacgccgag cccgtcgtcg agctcgtcgc cgacggaaaa gtcgtcgcca ccgtcacggg
    22921 cgggcagtgc ggggacgtca ccggcaggaa gatcgaggtt cacgcttccg acgaccgggg
    22981 cgtcacggtg ggctacaccg tggtgacgcc ggcgcgctga accgcgcccc cttttcggtc
    23041 cggcccgacg ggccgcaggc gcgttatcct gcgggcacga tgcctttccg cacgatcatc
    23101 gagccgttcc gcatcaagtc cgtcgagacc gtcaaggaga cgacgcccga gtaccggcgc
    23161 cgcgcgatcg ccgaggccgg atacaacgtc ttcctccttc ccgccgcgga cgtcctcatc
    23221 gacctcctga ccgactcggg cacgggcgcg atgtcggccg cgcagtgggg cgcgctcatc
    23281 cagggcgacg agtcctacgc gggcagccgg tcgttctacc gcttccgcga cgtcatccag
    23341 gacctcacgg gcttcaagca cgtcatcccg acccaccagg gccgcgcggc cgagcgcatc
    23401 ctgttccaca ccgtcctcaa gaagggcgac gtcgtcccga acaacaacca cttcgacacg
    23461 acgcgcgcga acatcgaggt cgagggcgcc gaggcgcgcg acctcgtgat cgccgagggc
    23521 cgcgtcccgt cgctcatcca ccccttcaag gggaacatcg acctcgccgc tctcgagaag
    23581 acgctcgtcg aggacggcga ccgcgtgccg ctcgtcatgg tgacggtcac gaacaactcg
    23641 ggcggcggcc agcccgtctc gctcgagaac ctccggggtg tgcgggcgct ctgcgacaag
    23701 taccggaagc cgttctttct cgacgcgtgc cggttcgccg agaacgcgta cttcgtgaag
    23761 cgccgcgaga aggggcagga gggccggacg cccaaggcca tcgcgcagga gatgttctcc
    23821 ctcgcggacg gctgcacgat gtcggcgaag aaggacggcc tcgcgaacat cggcggcttc
    23881 ctcgcgatga acgacgacgc gtgggccgag aacgcgcgga acctcacgat cctcacggaa
    23941 ggcttcccga cgtacggcgg cctcgcgggc tacgacctcg aggccattgc gcaggggctc
    24001 gtggaagtgg tcgaggagca ctacctccag taccgcatcc gctccatcga gtatctcgcc
    24061 gagaagctcg aggcgggagg cgtcccgatg atccgcccct ccggcggaca cgccgtctac
    24121 ctcgacgcgc gcgctcttct cccgcacatc ccgcccgcga ggtaccccgg gatcgcgctc
    24181 gtcaacgccc tctacgaggc cggagggatc cgcggagtcg agatcgggac ggtcatgttc
    24241 gggatgaagc ccgacgggcg cgaggagccg gggccgatgg acctcgtgcg gctcgcgttc
    24301 ccgcggcgcg tctacacgca gtcgcacgtc gactacgtgt ccgaggtcgt gctcgccgtt
    24361 gcacgtgatg ccgcgagcct gcccggctat cgcatcacgt ccgccccgcg cgtccttcgg
    24421 cactttaccg cgcgcttcga gccgatcgcg ccttgagagc gatcctgtcc ctcgccgccc
    24481 tactcgtggc cgccgcaccg ctcgcgcgcg cgtccgccga cgtcccgccg gccgcgttcg
    24541 cggatcccgg acgccgggcg aagctcctcg cggcgctgcc cgcggtcgag aaggccgtcg
    24601 ccgcggccgt cgagaagaag aagtttccgg ccgccgcgtt tggcgtcgtc gtggacggcg
    24661 atctcgtcct cgcgaagggt tggggcgcgc agggcccgtc gggcggcgcc gtggatgcgg
    24721 acacggtctt ccggatcgcg tcgatgacga agagcttcac ggccctcgcg atcctccggc
    24781 tccgcgacga ggggaagctc tccctcgacg accccgtctc gaagtgggtg cccgagctcg
    24841 ccgggatgcc gaaggcgacg gcggactccc cggagatcac gatccgccac ctgctgactc
    24901 attccgcggg gtttcccgag gacaacccgt ggggcgaccg gcagctcgcg cagagcgacg
    24961 cgttcctcgc cgcgatggcg cgcgccggga ttccgttctc gaacgcgccg ggcgtcgcgt
    25021 tcgagtactc gaacacgggc tacgcgctcc tcggtcgcgt cgtggctcgc gcgtcgaaga
    25081 tgcgttaccg cgactatgtc gacgcgaaca tcctgaagcc gctcgggatg acctcgacct
    25141 tctgggaggc gtcggccgtg ccgcccggcc gcctcgtgac gggaatgcgg ctcgagggcg
    25201 agacccctgt cgacgagccg ctcctcggcg acggggcgtt cggcgcgatg ggcgggctct
    25261 tctccaccgt gcgcgacctc gcgcggtaca cggcgttctt cctttcggcg tggccgccgc
    25321 gcgacgaccc cgacgcgggc ccggtctcgc gcgcgtcgcg ccgcgagatg cagcaggccg
    25381 cgcgcaccac cggggcctcc gtgaagatcg cggccgacgg caagccgcgc ctcaccgcgg
    25441 gcgggtacgg ctacgggctc aacgaccgcg cgacctgcca gttccgccac gtcgtgtcac
    25501 acggaggcgg cctgcccggc tacgggtcgc tcatgctctg gctgcccgag cacggtgtcg
    25561 cgctcgtggc gctcgcgaac ctccggtacg ccggctggag cggcatcttc aacgacgccc
    25621 tcgacgccct cgcagggggc ggcggcctca aggcgcgcgt tccgcaggcg gctccggctc
    25681 tgagggccgc ctacggcgcc gtgaatcggc tctacaccgc ctgggacgat cgggccttcg
    25741 acgccctcgc cgcggacaac ctcgtcctgg acacgccctt ggagaaacga cgccgtgagt
    25801 tcgcggatct gagggcgaag cacggcgcat gcgtgccccg cgcactcgag gccgagaacg
    25861 cgctgcgcgg ggagtggacc ctcgcctgcg agcgcggtga gatcgccctg aagatcacgc
    25921 tctcgccgac gcaaccgccg aaggttcagc tgctcgaggc gaagtcggac ccggcgcggg
    25981 ccgccgacga gccctgcgcg ccctgacgaa agaaaaacgc ctccgggcgg aggcccggag
    26041 gcgtgaaaac ggccgcgcgg cggccggatt ggaacgacgt tacttcttgg cctcgacgac
    26101 cgggaccttc gtccagtcga tcttcccgtc cttgcccttg gcggccttga ccttgtcgcc
    26161 gagctcgttg agggcggccg tctccttctt gggcatcttc tccttgtggc agctggcgca
    26221 ggacgcgttc gggtccttga tgtccttgcc gtcggcgccc ttcttgtagc ggttcatgat
    26281 gtccatggtc gcgaaggcgg agctgcccat cacgagcgcg gcgatggcgg cgacggcggt
    26341 ggtggtcttc ttcatgttct ttctcctccg ttttgtcccg tcatcgggac tcggttcgtg
    26401 ttgtctcaag tccggtgcca gccgcgcggc gaacggactg tgttgcatcg acgggggttg
    26461 cgcggccgga cccgattgga tccgcgaatg atcgccctgc ccgggcggca gatcacacga
    26521 aatcgtgctg attcagatgc ttcattgaac cggcgaaccg gctccgtcaa aggaccatcg
    26581 tctcgtggat cagggcgccg cggcggaagc ggtcgagcgc gagcggcgcg acgtccaccg
    26641 tctcgcaccg cccccggagg atcagctcgg cgatcgcccg ccccgccgcg ggggcgtgca
    26701 tgacgccgtg gcccgagaag cccgacgcga cgaagaggcc cgccatttcc ggcacggcgc
    26761 ccacgacggc gtggtggtcc ggcgtgactt cgtagaggcc ggaccagctc ctgcgcatgt
    26821 cgagcccggc cgccgcgatg gacgggaacc gggcttcgag gggctcggcg aagcgcagga
    26881 agaagtccgg gtcgaacgcc gcattgaagc cgggcggctc gtccgcgttc gaccatgcga
    26941 tcgcaacgcg gttaccctcg cggcgcacga ggacgccgct gtccgcgtcg atcgtcatgg
    27001 gaatcacggg cggcaggtgc gggcaggggc cgctcacgaa gatgtgccgg cggacgggct
    27061 cgacggggac gtcgaggccg gcgagagccg cgacccggcc ggtccagggt cccgccgcga
    27121 tcacgacggc gcccgcctcg accgcgccgg cttcggtctc gaggcggtag tcggcgcccg
    27181 cgcgcgcaat ggccctgact cccgcgccgt aaagtgtcct cacgcccgcg cgcgcggcct
    27241 cgcgcaggaa gaagttcgtc agtccgccgg ggtcgatgaa gccgtcccgc gggccgaacg
    27301 tcccgccggc gatcgggcct tccgcgtacg gcgcccgcgc gcgcaccgcc gccgggtcga
    27361 ggacctcgac cgcgacgccg cgggcgcgct ggaacgcggc cgccgattcc atcgcggcga
    27421 gcttccccgc gtcgcccgtc aggaagaggt agcccgcctt gttgtacatc ggcggctggc
    27481 cgatctcgcc cgcgagcgcg tcgaggatct ccatcgaggc gagcgacatc gcgacgttga
    27541 cgtccgtcgt gaactgcgca cggatgccgc cgttcgcctt cgacgtcgag ccggtgccgg
    27601 gcagcgattc cttttcgaga aggacgaccc gcgtcgcccc gagccgcgcg agctgaaacg
    27661 cgacgctcgt tccgatgacc cccgcgccga tgatcgcgac gtccgctcgc tccggcatcg
    27721 tgtcgagagg ctatcaggcc ggacggggcg ccggcccgcg gtcgggccgg cgccgcgggg
    27781 gaccgctaga ccagcgcggg gacgaaatcc tccgcgtgca cgtccgagcg cagcggcacg
    27841 gccacggtcg gcaggtacgc ggggttccac atcgtggcgg cgacggcctt cgggatctcg
    27901 gcgtccggga tggccttgcc gagcccggag tcgcgcgcca ccttgacgac cgcgaccgcg
    27961 acggcggcgc tgacgcgccg gatctccgcc gtggagggga agaggctgcc ggacgcgaga
    28021 tccgacgcct tcacctcgtc ggcgagggcc ttggcggccg cggcgaacat ggcctcggtc
    28081 acctccttcg cctccgagac gaggaccccg agcccgacgc ccgggaagat gaatgcgttg
    28141 ttgccctggc cgatgacgat cttcttcccc gcgtgcggca cggggtcgaa cgggctgccg
    28201 gtcgcaatga gcgcgcggcc gtcggtccac ttcgtgatgt cgaccggctt cgcctcggtc
    28261 ttgctcgtcg ggttcgacat cgggaagatg acgggccttt cgacgtgctt ggccatctcc
    28321 cggacgatcg cctcggtgaa cgtgccgggc tcgccggacg tcccgacgag catcgtcggc
    28381 ttgacggcct tcacgacggc gaggaggtcg cgcggcgcgc cggtgccgag gccgtacttc
    28441 gcggcgaggg ccgcgggcca cgcgaacggg cgcttgtgga cgtccttgat ttcgacgtcg
    28501 tccacgacga gaccctggct gtcgaggttc gcggtcgcga gcgtgagcgc ctcgcccgag
    28561 acgccggcgc gggagaacgt gtcgcgcagg aggcgcgcga tgccgattcc cgcggcgccc
    28621 gcgccgagga tgacgacgcg ctgctccttg accgggatcc ccgtcacgcg gcagccggcc
    28681 atcatcccgg cgacggcgac ggcggccgtg ccctggatgt cgtcgttgaa gcaggtgatc
    28741 gacttctggt agcggtcgag caggcggaac gcgttgacct tcttgaagtc ctcccactgg
    28801 aggaggacct tcgggaagcg gcgcttgacg gcctgcacga actcctcgac gagcgagtcg
    28861 tactcggcgc cgcgcaggcg cgggaagcgc cagccgaggt agagctcgtc gttcaggagc
    28921 gactcgttgt ccgtcccgac gtcgaggctg atcgggagcg tctgcgccgg gtggatgccc
    28981 gcggcggccg tgtacagggc gagcttcccg atggggatgc ccatgccgcc cgcgccctgg
    29041 tcgccgaggc cgaggatgcg ctcgttgtcc gtgacgacga tcaggcggac gtcggcgaac
    29101 ggcgcgttcc cgagaacctc gtagatccgg ccgcggtggc cgggcgtgat ccacaggccg
    29161 cgcgcgcgcc ggaagatgtg gctgaactcc tggcacgccc gcccgaccac cggcgtgtag
    29221 acgatcggga ggaactcctc gatgtggtcc acgagcgtcc ggtagaacag gtgctcgttg
    29281 cggtcctgca gggacgcgag cccgatgaac ttctcgagct cgtccgcctt gcgcgagatg
    29341 ttcgcgtaga cgcggcgctc ctgctgctcc atggtgctga cggcgtgggg caggagtccc
    29401 tcgaggccga acgcttcgcg ctcttcctcc gtgaaagccg tgcccttcgt gtacatgggg
    29461 tggcggagca gccgtgtgcc gcggtagggc acctccagcc gggccgcgcc cttggaatcg
    29521 tgccgaaccg aatacttctc catcgtcgga agcctccact cacaacgtgg gcctccggcg
    29581 tgccccgggc atgaggcatt tgcttcagtt gggcagcatc acgaccagga ccttgctcgc
    29641 cttccagaag cgggcggcgt ccgtgacggt gagcgtgctc gccttctccc ccgcggccgt
    29701 gagcgtgtag ctgtccttcg ggtggtccga gaggacccgg gccttgtccg ccgcggccgg
    29761 gatgtcgaac gtcgtgacct ttcgcgtgtc gaccggcttg aacaggtttt cgtcgaactg
    29821 gcccgtctgc ttgtagttgc cgccgaggcc caggaccgcc cctttcttct cgacgatgcc
    29881 cgccttcttc agggtctctt tcggcgcgat gaggacgtag gcggtcgcca tctgcgcctc
    29941 ccggtccgcg atcacggctt ccttctgctg aacggtctct tccttctcct tgaccgttcc
    30001 ccgcagctcc tcggccgtct tcgtgagttc cttcgtcttt tcctcgaggg cggcgatggt
    30061 ggcttccttt tcctcgagct gcttccggag ctcgccgacg agcctttcga gcgacgtcac
    30121 cttcgcatcc gaggccttca gcgatttctg gagggtcgcg agcttcgcga ggttcgcggc
    30181 gacggccttg cggatgtccg cgatctcggc cttcagctcc tcgcgctgcg ccgtgttcgt
    30241 cttgccttcc tgcgcgacgg cgatcgacgt cttgaccgcc ttgagctcct tgacgcgcag
    30301 ctcctcgagg cccttctgca cctcgtcgag cgtcgcctgc atctccgcgg tggcggcgcc
    30361 ggcggcgctg agctcccgct gggcgcgcgc cttctcctcg gtcagcgcct tgttctcggc
    30421 ctgcgccttc tcgaggtctt tcttccatgc gcaactcgtc aggacgagcg cggcgaggaa
    30481 cagggcggga atgcgggcga aaagcatcgg tcgtttcatg gcgtcacctc gttgcgccca
    30541 tcgtacctcc ggggcccgcg cagcggtccg cggtgagaag attcgcacgt cacgattgag
    30601 aaggagaacg gtatgaagcg gatcttcgtg ggtttcgcgg cgctctggct cctcgcggcc
    30661 tctcccggcc tcgcgcagga cgcggagggc tgcaaggatc acccgctgtt cacgcggttc
    30721 ccgaacacgc acatcatcga ctgccagagc agccagttcg acctgcgaaa gttccccgtc
    30781 ggccagcagg gaaagggtga cgagaccctc aagtcggtcg aggtggaggg tccggtgcgc
    30841 tggctcggct acgagctcaa cgaaggggcg acgccgccca gcggtctcca gatcatgcgc
    30901 aacttcgaga acgcggcgaa gaaggccggc ggcaccgtcg aggggcagta ccccggctgg
    30961 tgcaaggcca tgtacgaccc cgagggaatg ccgcggatgg ggaacggctg cacgagctac
    31021 gccctgacgc tcaagttcct gcgcggcggc aaggagacct gggtcttcct ccaggccggc
    31081 gaaaacgacg ggtcgtacgt gatgacggtt tccgagcgcg aggcgatgaa gcaggacgtc
    31141 accgtgaacg agctcgccga caagctcacc aaggacgggt tcgtcacgct ctacgtcaac
    31201 ttcgacaccg gcaagtcgac gatcaagccc gactcggcca agacgctcga cgacgcggcg
    31261 gctgcgctca aggtggccgc gactcttcgt gtcgaggtcg ccgggcatac cgacaacgtc
    31321 ggcgcgcccg aggcgaacca gcgcctctcg caggagcgcg cgcaggccgt gatggccgcg
    31381 ctcgtggggc gcggcatcaa ggcggaccgg ctgactgcga agggctacgg gcagacgacg
    31441 cccgtcgccg acaaccgtac ggaggaagga cgcgccagga accggcgcgt ggagctcgtc
    31501 aagaagtaac ggctccgacg aacgctagga cgcgccgcgc ctggacgcga cgtagagctt
    31561 ccggaccggc cgtcccttca tccgctggat cacgcggatc gtctcgaggc gaagccccga
    31621 gacgcggagc atccgtcgaa cctccgcatc ttcgaacgag cgctccttca cggtcacgag
    31681 gatcttccgg tagagcctcc cctgcttcac gaaccagagc tggttgaagg tcgcgatgcc
    31741 cgtcttccgg tcgaacgcgt tggtggccat gaagacgtcg gggccggcct cgaacagctt
    31801 ctcgctcgtg ctcagccact cgagccagga ccgtgtggtc aggtcgaaca ggaacaggcc
    31861 gccggggcgc aggagccgag ccgtgttcgc gaagatccgc tgcacgtctt tcgcgtcgag
    31921 aatgtggttc agggcgtcga agtggcaggt cgcgacgtcg gccgtcacgt ccagcgagag
    31981 tctggtcagg tcctgcctga cccaccggac gcgtcgcgac tttctccgtg cgacgcggag
    32041 catcgcttcc gacacgtcga cgccgatgac cgtgcgcccg cgcgcgccgg cccagggaat
    32101 ggccgtgttt ccggttccgc acgcgaggtc gacgatcacg cgcgggcgca ggcgatgcgc
    32161 ggccgccgcc gtcgtcaggg ctcgccggaa ctcccggtag atcgcctgga tgccgggatc
    32221 ggcggccatc cggtcgtatc gcggcgccag cggaacatag gggtcgcgca tgcggccatt
    32281 ctgatccgaa ggccgggcgc cgatgaactc cttcgaaacc ggcgtcagcc ggggacgacg
    32341 tcgaggctgg ccgtgtgccc ggccatcacc tgccagccgg gcggcctctt catccacacg
    32401 ttggtggttc gatagccgcg cttgatctcc tgaccctggc tctggatcgt gacgacgaag
    32461 cggtagctcg tgacggcagt gtccccgtgc gcgacgacct tgacgtcgtc cttcgcgtac
    32521 gacgccacga acggcttggc cccgccggcg gccgcctggc gctcggcggc gccccgccgc
    32581 gcctcgtcca tgagactcga cttgccgcag gtggcgccgg tcaaacccgt gaacaggatg
    32641 tcgtcggcat agattcggtc gagagcgtcg agatcgatct cgcgcgtggc ccgcgtcagt
    32701 cgttcctcct gtgccaggat ttcctgctcg agcgtgccgg tcatgggtcc ctcctagatg
    32761 gtgatttcgc ctgctctcat gagtacgccc ggtgcacgcc ggcgtcccga aggttcgcct
    32821 aagattcgcc taagattgag tctcatggcc gagcgggagt cgtaccggtt cggagacatg
    32881 accctcgacg tggccgagcg ccgcctctcg agaggcgggc gaagtgtccc cctggccccg
    32941 aaggggcacg acctcctcgt tcacctggtc cgcaatgcgg ggcggctcgt cacgaagggc
    33001 gagctgctcg agcacgtgtg ggcggggtcc ttcgtggagg aggggatcct gtcggtccac
    33061 gtctccgcgc tgcgcagggc gctcggcgac gacaggcgat cgcggcggta catcgaaacg
    33121 gtttcgcgcg cggggtatcg cttcatcgca cccgtccaag acgacggagc gagggacggc
    33181 cgctcgcttg ccgttctccc ggcgtggccg gccggtcccg ggacctccga tgccgaacag
    33241 tcggtgggcc tggccctcgc ggacgccgtg atcgcccgtc tcgggagcct gacgcggatc
    33301 tcggttcgac ccacccgggc ggtccgctcc tacgcggatc tccagccgga cccggccgcg
    33361 gtcggccgct cgctgggcgt cgacgcggtg atcgtctgca agttcgagaa gacaccggag
    33421 cggatccgcg tcgccgccga gctcctccgc tctcgcgacg gcgtccgcct atggagcggc
    33481 gcgtacgacg agctcccggg cgacgggact tccattcctg atgcgatggc cgaagccctc
    33541 gcggcccatt tcgccgcaac gcccctgcgc ggcggttccg ccggcgtgtt cgggaactgg
    33601 gccgacgtcc ggcgcagcgt ttcacgccct ccgtgtcgcc tcgaggtgta cgagctgatc
    33661 ggacgcggac gggcccatct gctggccttc tcgagaaccg agacgccgaa ggccatcgcg
    33721 gcgtacgagg ccgccatcgg actggatccg gccttcgcgg ccgctcatgc gggtctcgcc
    33781 atggccttct gccagcaggc cgagcttcga ctcgtcccgc aggcggaagc gtatgcgcgc
    33841 gcgaaagacg ccgcgcttcg ggcgctcgcg gtggacgatg cgtgcgcgga cgcacaggtc
    33901 gcgctggccg cggtctcttt tctcggccaa tgggactggg tcggcgccga gcgggccctg
    33961 cagagggccc tcgagatcaa tcccaaccac accgaggcca ccgtgctgta cggacggctt
    34021 ctggacacgc tcggcaggct cgaggaaggc ctcgagatga agatgcgcgc tctcgagcgc
    34081 gatccgctct cgcctctcgt gcaccaggcg atctcgatgt cgtactggca tcaacgccga
    34141 tacgaggatt ccatccgttg ggcgaacaaa accctcgagc tcgacgcggg acacctcgtc
    34201 gcgcgcgagc atctcgccgg cgcctactgg gccctcggcg acttcgatcg gcacatggcg
    34261 gagaacgtca aacatgcgga gtccttcggt gtggcggccg aggccctcga accgatcaag
    34321 caggcctacg cccgcggcgg acgcacggga ctcgtccggt ggctcctcga gacccagggc
    34381 acccatctgc ccgcgatcca gctcgctctg ctccacggcg agttgggcga tctggaggcc
    34441 gccatgcggc atctggctcg ggccatcgac ggccatgagt cctgtctcgt ggatctcgcc
    34501 gtcgctccgc agtgggaccg cctgcgtgaa agtccggact ttcaagtctg tctggcgcgg
    34561 atgggcttgc ttgcgcggga ccccggatcg acttcgcgtc agaaccgacc gtgaccggcc
    34621 tggcctgcgc gccctcttca cggagaggac ctgagcggtg cgggacgggg ttccgggccg
    34681 accgcgtgct cagccgcctc catggagccg cctgagggcg gcctgcttca gggccatgct
    34741 cgtcgccgcg ccgcgcgcga tcgccttgat ctcggcctcg ggaaggcccg gcaggatccg
    34801 gagcgcgaag acctgtggcg tcttcgggtt gtggacgagc gcggccttca cctcggcgtt
    34861 cgccgcccat tggctcgtct tgaggagcag ctcggcggtc tgatacgtga gagtcgccga
    34921 cttcgcgacg cgcacgactt cgtccacggt gaggcgcggg ttcttcagga gcgcgtagag
    34981 gacctgcggg tcgccgtcct ggacgaggat gagccggtcg ttgcggtcgg cgcgagcggc
    35041 gtggaggagc ttctccgtcc gcgacattcc gcgcaggcgg tcccagtcgc tcacgttgcc
    35101 gggcgcagcc ggcggagggg ccggggacgc gagcagcgcc tcgaggagcc cgcccgcgtc
    35161 cccctcgacg tggagcgcca cgccgtcggg cagcggcgcg acgaccgtcc ccttcacctt
    35221 cgcctcgccg ccggacgggc cgcggagcgt cacctccacg ggcgtgaagg gcgcgggccg
    35281 ctccgaaacc ggcagcctca gcccgcgctg cgagaggttc gtccggtgct cctcgcggag
    35341 ctcctcctcg gtttcaaact ccagcgtgaa cgcgttcggc ccgatgcgat cggccttcac
    35401 cgacccagtg taggcgagga ggccacgagc ttggatgaag ggctggtaaa gtcccgcgcc
    35461 tcgtacaccg gacgaggcac gcgtcgagcg gccggggaga agtcgagtcc tgcggaagac
    35521 ggcgcgagcc gcgtcgggga agaggttggg gagtgacgga gaagaggacg gcgccaggca
    35581 gaaccgtttc gggggacagc ttcttcggga gggtcctcgg ccttgtcgag cgcaccggga
    35641 atgcgctccc gcaccctgcg acgatattcc tcgtcctctc gctgggcgtt ctcgccgcct
    35701 cggccctcgc gtcctgggcg caaatctcgg ccgtccaccc cgggaccggc aagacgatca
    35761 cccccgtcaa tctcctgacc gtcgagggac tccaccagat cctgaacaag acggtgtcca
    35821 acttcacgag cttcgccccg ctcgggaccg tgctcgtcgc cctcctcggt atcggggtcg
    35881 cagaggggag cggcctcctc ggtacgctgc tgcggctcct cgtcctctcc tcgccgcgcc
    35941 gcctcctgac cttcgtcgtc gtcttcgccg gagcgatgtc gaacgcggcg agcgaggttg
    36001 gctacgtcct cctcatcccc ctggcgggcg tgatcttcct cgccgcagga aggcatccca
    36061 ttgcggggat ggcggccgcc ttcgccggcg tctcgggcgg ctacagcgcg aacctcgtcc
    36121 tcggaacgat cgaccctctc ctggcgggcc tcacgcagga ggcggcgcac atcgtcgacc
    36181 cgacgtacgc cgtcagtccc gcggccaact actacttcct cgccgcctcc acgttcgcca
    36241 tctcgtttgc ggggacctgg gtcaccgaga agctcgtcgc cccgcgcctc ggcacttaca
    36301 ccggctccga gaagggcgaa ggcgagatgc ggcccctcac ccccaccgag cggaaggggc
    36361 tccgcaacgc cgtcggcgtc ctcgccgtcc ttgccgtcgt gattctctgg ggcatcgttc
    36421 ccgaaggggg cttcctgcgc gatctgaagt cgggaggagt cctccactcc ccgctcctga
    36481 ccgcgatcgt tcccttcatc ttcctcgggg gagccctggc cggcgtcgcc tacggcctcg
    36541 gcgccgggac gatgaagagc gacaccgacg tcatgaaggc gatgacgaga tcgatggaga
    36601 cgctcgggag ctacatggtc ctggtcttct tcgcggcgta gttcgtcgcg ttcttcaact
    36661 ggtcgcacct cggcctcatc ggtgcggtca agggagccga ggtcctgaag gcaaccggtt
    36721 tcggcggcat cccgctgatg ctcggcttcg ttctcctctc ggccggcgtc aacctcctga
    36781 tcgggagcgc ctcggccaag tgggcggtca tggccccggt cttcgtcccg atgctgatgc
    36841 tcctcggcta cacccccgag ctcacgcagg cggcctaccg cgttggcgac agcgtcacga
    36901 acgtcatctc gccgacgatg tcgtacttcg ccctcatcct cgccacagtc caacgttacg
    36961 actcgaaggc cggcctcggc accctcatcg caacgatgct gccgtactcc ctcgtcttcc
    37021 tcgtcgtttg gagcgtcctt ctcgtcgcct ggatcgccct cggcctgccc ctgggccccg
    37081 gcgccggcct gcggctctag ggcctgaatc ggaggcggcc gcgacccgct gagcggccta
    37141 gccgttcccg gacacgggct tccggagctc ggcctcgatc gcttcgagct ctgccctcat
    37201 tccggcgagg cgccgcgcga gaggctcccg ctccgaaccg gccgccagct tctgctcgcc
    37261 atttgccgtc tcgaggttgt tgatcacgat ggcgatgaag agattgatga cgacgaagac
    37321 ggcgagcacc acgaagctca cgtagaagag ccaggcccac cgggtggccg cgagtgaggc
    37381 gtccatgagg tcgacccagc cctcgagggt gaggatctcg aaaagggtca gcccggcccg
    37441 ggggagcgtc ccccagtggc caggatcggc ccccccgaag aagtgcacgc ccaggaccgc
    37501 gtacacgtaa aggatgatgc cgaggagcgc aacgacgttg gcgagcgaag ggatcgagcg
    37561 gagcatcgtg gccacgatca ggcgcatttc gggcagggcg gagacgaccc gcagcgcgcg
    37621 cagcaggcgc gcgagccggg cgaccgtggc gaagggtccc gcgacgggaa gaagcgagag
    37681 cgcgacgatc gtgaaatcga aaacattcca gccgtcgagg aaaaagcggt ggattcgcgg
    37741 agcctgagcg aagaggcgcg ccgcgatctc gccgacgaag acgacctgaa ccgccaggtt
    37801 gagggtctcg aacaaacgtg gccggggccc gtctccgtgt tccgcgcgcc gtacgatgag
    37861 cgccagggag gcagcccgat gacgcgatcc gcgaacgccc gagtcgccgg cttcacgttc
    37921 ctcttctaca tcgtggcggg tatcacctcc atgatcctga acggccgggc cagcggcgga
    37981 ccggatcccg ccgccaggct ccaggcaatc tcgcgccacc cgaccgagat ggccgtcgtc
    38041 gtcatcctcc tgctcttcca gtgcttctcg gccctggtcc tggccgtcac gctgcacgcc
    38101 ctcacgcgcg agacggaccg cgatctcgcg atgatgggcc tgctctgtcg cgcggccgag
    38161 ggcatcctca gcggcgccgg cattgccagc acgctggccg tcgtcgacct cgcgcgaacc
    38221 acgggagtcg gcggttccga tcttccggcc aggcatctgc tcggcgccca cctgcttcgc
    38281 gaggacatcg ccctcaccgc gaccttcttc gccgtgggca gcctgctctt ctcgctcctt
    38341 ttcctccgcg gccggctgat ccccgcgccc ctcgcctggc tgggagtcgc ggcgtcggtc
    38401 ctcctcgtgg tcgagcttcc gcttcagctc gcgggattcg tccacggccc cgtcacccgg
    38461 ctcgtgtggc tccccatgct tgccttcgag gtccccctcg gcgttctgct cctcgtcaag
    38521 ggtgtgaggg ccccggcgac cccggccacg gcgccgctcg gactcgcagg ttccgcaggc
    38581 cccccggcac gaccgtagaa ctggcctgcc cggggagatt cgaactcccg accctcggct
    38641 tagaag
//
LOCUS       DAOMFY010000008        38218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_336_length_38218_cov_8.228965, whole genome shotgun sequence.
ACCESSION   DAOMFY010000008 DAOMFY010000000
VERSION     DAOMFY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 38218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38218
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_336_length_38218_cov_8.228965"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="PLB01_01570"
     CDS             <1..1430
                     /locus_tag="PLB01_01570"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQQ76016.1"
                     /translation="AVARGAVAALGAAPAPENLSVPYLAVENPRRAAGLMAALLAGRP
                     ASRLVMAGVTGTSGKTTTAFLVDRVLAARHERRGLFGTIVYRGAGGDAAAVASPHTTP
                     EATELQPMLGALVADGGTAATLECSSHALVLERLAGSTFDVALFLNLSHEHLDFHAEM
                     DDYFEAKARLFALLKPEGRAVVNLGDEYGRRLTSRLVAGRTVGFFLEGEEGAEQTRPA
                     ALAGAVAVAAAVAGGAETGGLFATVLGRATLGLAGTRLEIEVKTPFTFKENLLFSINS
                     PLLGRPNAENLLAAAAAGIALGFTPDEIAASLSSVSVVPGRLERIENDRGLTVLVDYA
                     HKPAALEGVLRTVRPLAQAGGGRVHVVFGCGGDRDRAKRPVMGRIAAQLADDVVVTSD
                     NPRSEDADAIIAEVLGGVPAGADVAAVADRRNAIAEALRHARPGDVVLVAGKGHETEQ
                     VIAGVAHPFDDRAVLRALLAGEAGR"
     gene            1427..2512
                     /gene="mraY"
                     /locus_tag="PLB01_01575"
     CDS             1427..2512
                     /gene="mraY"
                     /locus_tag="PLB01_01575"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQQ76017.1"
                     /translation="MIPWLLHPFFGKVPVLGVFRYITFRAANAALMALLLAVFLGPSL
                     IRWLRSKQIGQSIREEGPKAHQAKAGTPTMGGVLINLAIVVPTLLFADLTNRYVWVAL
                     GATVLAMVIGIGDDWRKVSRKQNRGLTGRQKLVLQAVIGLAVGWAAVNAFDGRVDATH
                     VAFPFLKNVHPDLGALYVLFAAVVVVGSSNAVNLTDGLDGLAIGATLIASGTFAILAY
                     IAGNARAATYLLVPYVPGTGELAVFCAAIVGASLGFLWFNAHPAEVFMGDTGSLALGA
                     ALGTVAVLIKQEILLVLVGGLFVVEAVSVILQVGSFKLTGRRLFRMAPLHHHFELLGW
                     PESKVIIRFWIVAILFALVALGSLKLR"
     gene            2509..3915
                     /gene="murD"
                     /locus_tag="PLB01_01580"
     CDS             2509..3915
                     /gene="murD"
                     /locus_tag="PLB01_01580"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQQ76018.1"
                     /translation="MSVDVGPDLPEIRAAAVFGLARSGRAAVTALAERGVAVTGTDAK
                     DASELGELPALDGVRWELGGHPLSLLDGVDLVVVSPGIPLTLPVFDAARARAIPVVSE
                     VELASRLLPGVVVGITGTNGKSTTTALAAALLKAAGHAAIACGNYGTPWISFATKAGA
                     NGTGSAPKTWVVELSSFQLEGIRRFAPDVAVHLNLTPDHLDRYRSMDDYGAAKARIFE
                     NQREAQVAVLNADDPLVARIRPRARRFEFSRAAQPALGTWLKDDLFVADVTGKGPRVI
                     ARRSDLALPGAHNVENALAALAATLPLGVTPESAVETFRGFRGLPHRTVLVRTLGGVT
                     WWNDSKGTNVDATLKSLEGFDDARVHLILGGKDKGDDFGRLAPLAARKARTILAIGKA
                     APAIARALAPVPGLDVVLAGTLERAVEEAARRAGAGDAVLLSPACASFDQFRNFEHRG
                     DAFEELVRALPELAGKVG"
     gene            3915..5018
                     /gene="ftsW"
                     /locus_tag="PLB01_01585"
     CDS             3915..5018
                     /gene="ftsW"
                     /locus_tag="PLB01_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQQ76019.1"
                     /translation="MAKKLASDRLLFGSVLLLVGTGLLMIYSASSLQATLPSSSAPAG
                     PYFFVLKQTIAVAVGSLLAFAAMSIDYRRLDDPRLVGVILAVLAAALVAVLFRAPVNG
                     ARRWLALGFMSVQPSEFMKVGLVIALAALLSRREGEMHLPTRTLLPVLGLVVVPAGLV
                     LLEPDFGTAFTYFIVAGAMLFFAGLPFRWFLGSALLLVPTLSAWVLSAQYRRDRLTAF
                     LHPEMDPLGKGFHAIQSLIAVGTGGVTGLGLGEGKQKLFFLPYPYTDFIFAIVGEELG
                     LVGCVAVLALFGLVFARGVRAAVNAPDSFGRLLGIGLSVMIVVQALINISVVLSLVPT
                     KGIPLPFLSYGGSALWADLLAVGILLNLSQHAS"
     gene            5015..6133
                     /gene="murG"
                     /locus_tag="PLB01_01590"
     CDS             5015..6133
                     /gene="murG"
                     /locus_tag="PLB01_01590"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQQ76020.1"
                     /translation="MTSAASSPATGGFLIAGGGTGGHVFPALALAGEIRRRRPDAPIV
                     FVGTRRGLEADLVPRAGWPLEFVRAKGLVGKGARARVEGLLALPLAFADSIGILGRHA
                     PRTVVGVGGYASGPLVATAWARRIPTVIHEQNAVPGLTNRLLSKVATRVATGTDAGAA
                     RLGGTCVGNPVRGEFFEVSSLGGRETPRRMRVLVVGGSQGASILNRLVPGALARVDAA
                     GVRFAAIHQTGRGRAADVAAAYGRHAFSVSAVLPVEFVEDMAQAYAEADLLVARAGAM
                     TVAEIAASGRPAVLVPFAGATHGHQEANARALERDGAAVVLTEDESSEESLGNAIAAL
                     LSDPARLAAMGDAARRSARPDAAARLCDVVFEAEVSRI"
     gene            6130..7608
                     /gene="murC"
                     /locus_tag="PLB01_01595"
     CDS             6130..7608
                     /gene="murC"
                     /locus_tag="PLB01_01595"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQQ76021.1"
                     /translation="MNFLRVKRLHFVGIGGIGMSGLAELLKSVGLEVTGSDLSEGEQV
                     LRLRTLGIPVFLGHHAENVQGADVVVYSSAVHDSNPEVATARSMGIPVIKRAEMLAEV
                     MRVKRGIAIAGTHGKTTTTSMTGAILMEAGLDPTIIVGGRMREVGTARLGRGEYLVAE
                     ADEFDRSFLALSPMLALINNIDLEHLDTYRDLQDLQETFARFARSVPFFGAAILGLDD
                     PHVQEIRPLVSRRVVTYGLTPQADLTARDVVLERTGSRFVAFADREFTGAVSLQVAGL
                     HNIKNALGALAVARELSIPFATAARALAEFKGVIRRFEKKGERAGVVVIDDYAHHPTE
                     VAATLAAARQSYPDRRIVALFQPHLYSRTKDMAHQFGAAFLNADVLLVTPVYGSREAP
                     REGVTGALVADAATARGHRAARFIADRKGILDALEWELKDGDLLITMGAGDVLHFGED
                     YLKKEGGVLERSGKLTAPAPAAHPQPTPSKKSESENKGSDKS"
     gene            7650..8414
                     /locus_tag="PLB01_01600"
     CDS             7650..8414
                     /locus_tag="PLB01_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76022.1"
                     /translation="MRRRAGRRLLVPALTLLFLSGLALSGLVILRTHPRFAVTRVVLE
                     GVPEALRAEAEELTDGWIGRPALFVDLQGPVEELSKRSWVASASARRVVPGTIVVVVV
                     ARPPVAVAIREDRGGELWTLDRTGSFIGPYGGRALGREDDFVVLSGASDGTARARGAA
                     FLDVLKTEDPDLLARASEVAVVPEGFAVTDRVASCRLLFGPDAAAPGRAAPIWRAYLA
                     LLPELERNAFPTSDADLRFAGRIVLKAPGDTGRGKT"
     gene            8425..9672
                     /gene="ftsA"
                     /locus_tag="PLB01_01605"
     CDS             8425..9672
                     /gene="ftsA"
                     /locus_tag="PLB01_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQQ76023.1"
                     /translation="MPKTTPYLVSLDIGSTKVCALIAESDATGRIDIIGKGTAAHKGA
                     RKGAIIDVPATVDAIKRATEEAEIMAGAQIERAVVGVAGPAVKSFNSRQVVAVAAKNR
                     EISREDIQRSLDAARSVPIPSEFEILHVLPREFVVDGQDGVADPLGMVGSRLEADVHV
                     VTVPVATTQNLLNCVNRAGVEVIEMVLEPLAASEAVLTADERELGVVLADIGGGTTEI
                     AVWRHGTLAHTSVLPVGGDHFTNDLAVVLKTPVPDAERLKRKHGAALAALVNESDSID
                     VPLTGGRGVHPVKRREIAEILQPRAEELLTLLWEESVREVPAGELRAGLVLTGGGSEL
                     DGLLEVAEQVLGVAVRRGVPRGLGGLTDIVASPEWACAAGLLLYGRGAAAAARRRNRG
                     ASPGFLDKIKGSFRNLFPSSTAL"
     gene            9692..10906
                     /gene="ftsZ"
                     /locus_tag="PLB01_01610"
     CDS             9692..10906
                     /gene="ftsZ"
                     /locus_tag="PLB01_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQQ76024.1"
                     /translation="MILFDDDDAGSKRTSRTITFSEDSAPPARIKVVGVGGGGGNAVN
                     RMIQAGIKGIEFVAANTDLQVLKVNRAATKLQLGITTSRGMGAGSNPDVGRNAALEDA
                     ERITEVLAGSDMVFVTAGMGGGTGTGAAPVVARLASEAGALVVAIVSKPFAFEGRRKL
                     RYAEDGIAELREYVDTLITIPNERLYSFVDRNVTTWEAFRIADDVLRQAVQGISDLIT
                     VPGIVNLDFADVKTVMENMGMALMGTGTATGEHRAVEAAQRAISNPLLEEQSIQGARA
                     ILINVTGGEDLTLAEVNEACAIIQQAADEEANVIFGLVQDKDMKDEVKISVIATGFDA
                     PAALASRREVSLPVNPSGARGAAPERPRPGAEPAMPPTPEDQGYGVNLINMRDPREDI
                     FDIPTFLRRQMD"
     gene            10973..12880
                     /locus_tag="PLB01_01615"
     CDS             10973..12880
                     /locus_tag="PLB01_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQQ76025.1"
                     /translation="MRSRGAFTGILLTLFLARVASLVFHFVLAAPSGARVAEHPLQLL
                     PAALAGEIGTLAALGALAGVLSWLSTRAGLLLLAGGGVFVLFLSQLDLELVRWTGEHL
                     NPQWIGAYNLLAHRRLLVDVVFGDTLALGAAVLLVAVPAGIVFALARRRGPERAAPLS
                     VAFLAAVSCGALTFESALAPTRLALRRARPVLVGLVSESVAALGDAPSPREVRAGIAS
                     LHRFLNRPPRWFPDAGYPVWHPAPAEAASVAAFRARPLEKRPDVLLVVLESARGWETD
                     VRRPGVESRIPEIHRLWKERGVAFPVCIASGYPSVEGRGGILLGVAGHPSGILLYNAR
                     ELNVLSIGELLAKAGYARELVAATSPAFEKMEYWYARWFDAVRYDAADTTDEDLARRT
                     IERLESRREAPLFLTLFTIATHPPVYRPPGSDPKTPRESYLAALGYTDRTLGRIFESL
                     RRTPRGRDTVVVVVGDHSTTNPWQSIRLPRLGTPNAGENWTTLVVAAPGLPAGTVREE
                     LSSQLDVGPTLLGLLGLDVSNHFFGRDLFEKPAPAPQGVLALRLRGLAAFEGPFLLQT
                     RLDDPSFAQKWRWQDYEASPDDDNGGYHHGTLQELTAADRARIEELKTMARAWGAVLD
                     GNRVMPPGATR"
     gene            13970..14761
                     /locus_tag="PLB01_01620"
     CDS             13970..14761
                     /locus_tag="PLB01_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76026.1"
                     /translation="MPPALLFSLLLVADAGTFDRAPKEAWEVRNSYDLSDQAGRVVVN
                     VTSRSYSLGAAASGDSTFRAQTEVTFGRSDGTIFIWRETLEPLSGDARGRMTVTLESL
                     ADHCKIELDTGPATQSGTYDRIRLQVPGAALVFAEVDAGLVERQLKSADLEAFREKVP
                     PGLREMCTFLVELSDVIPAPGGILRDWVTRIAAPAGTKEDPSRARKWSRENEQSQTSK
                     HVVPVLATPPPDSIRGPGDEIKKTKVSREERRPSGAAAPAATPGP"
     gene            complement(14700..15002)
                     /locus_tag="PLB01_01625"
     CDS             complement(14700..15002)
                     /locus_tag="PLB01_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HQQ76027.1"
                     /translation="MSAPLPSRGLVRRRSRPVRMALLALGWTLLALGVLGGLLPVIQG
                     WPFGVAGLAILYIESRWVQRKVRRFRQQNPRFERTWLKARAWLRERRRRKASVPLD"
     gene            complement(14999..15817)
                     /locus_tag="PLB01_01630"
     CDS             complement(14999..15817)
                     /locus_tag="PLB01_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQQ76028.1"
                     /translation="MTDSALPLDALGPVFELCTERPLAVFDLETTGTDRLSDRIVEVA
                     VVRFRKGGEVATLEKRVNPGVKIPRESTRVHGISDSDVAGCPAFPALAPELAAFLEGC
                     DLAGYNIRQFDVPVLLREFELAKVPFSMDGRRVVDMQTIYFRREPRDLGAAVRFFAGR
                     EHAAAHAALADAVASAEVLAGQLRRYADLPRDVAALHELSRPPEGRYVDPDKRFLWRD
                     GEVVFAFGQHRGTRLADVAEKNPSYLDWIVDGNFPDEAKRIARDAQRGVIPRRD"
     gene            complement(15825..16556)
                     /locus_tag="PLB01_01635"
     CDS             complement(15825..16556)
                     /locus_tag="PLB01_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HQQ76029.1"
                     /translation="MKPLPSPRGAEAYLAIDKPAGVAVHGPHGLIGPLRREFGDNLAL
                     VHRLDKETSGVLLLARTPEALSAALEAWPTSVTKTYLAVTRGVPSPPEGVVTEKLLEH
                     RTGKPELLKRALKAAYGPARAGHLLSGRRVLAIPPVPPPGRTAAHPAGREARTAYRVV
                     QDRGTTALVELTPGTGRMHQLRVHLAHLGTPLLGDPLYDAFRTPDSPAPVLHAARLVW
                     EGPPGAPGGSWTWEAALPGARRVGP"
     gene            complement(16553..16783)
                     /locus_tag="PLB01_01640"
     CDS             complement(16553..16783)
                     /locus_tag="PLB01_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HQQ76030.1"
                     /translation="MVGIATGAAYVPQALRIWKRRSSEDVSITTYLLFLGGQLVYLLY
                     GIRFHQTPIVIGMAANIGGSLAVIGSALKFRR"
     gene            complement(16836..17558)
                     /locus_tag="PLB01_01645"
     CDS             complement(16836..17558)
                     /locus_tag="PLB01_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HQQ76031.1"
                     /translation="MPTELPPRPFDSPRPIDPSAAVAIPSFLARVYVWMAAGLLVTAG
                     AAFATLSNESLLKLVFGNRMVFFGLMIGELALVAWLSGLVGRLSSGAAGAVFLFYSAL
                     NGLTLSVVFLAYTSASVVSTFVVTAGMFGAMSVYGLVTKRALDGLGSFAFMGLIGVIL
                     ASVVNIFLRNDMLGFVVSCVGVIVFTALTAYDTRKLKMMAATVDGASEEGKRGAISGA
                     LALYLDFINLFLMLLRLFGNRR"
     gene            17616..18071
                     /locus_tag="PLB01_01650"
     CDS             17616..18071
                     /locus_tag="PLB01_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HQQ76032.1"
                     /translation="MKRTASALLVTLLLLPLSLLADAGKFGAPLGNSPKVALADLVKD
                     PAAWSGKTVKTEGVVSSVCQEKGCWMVLKSGDQSVRVRFKDYAFFVPKDSAGATAVLE
                     GVFTVKTVPEATAKHYAEETPGGKPADIKGDQKELSFLASGVELSKPAK"
     gene            18114..20348
                     /locus_tag="PLB01_01655"
     CDS             18114..20348
                     /locus_tag="PLB01_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76033.1"
                     /translation="MVEQQDSLVGKSVSHYSVVRRLGSGGMGVVYEAEDSRLGRNVAL
                     KFLPPELARDGSSLERFQREARAASALNHPGICTVYAIEEHDGQHFIVMECLEGQTLA
                     QTMAARPAFDLESVLSVGIQIADALESAHARGIVHRDVKPANVFVNARGQVKILDFGL
                     ARIEGARRGNDGAVSRLETVGDLTTPGTAMGTVSYMSPEQARGQLADARTDLFSLGTV
                     LYELATGALPFPGETSAVVFDAILNRDPLPAAQQNPALPPELSRILDKALEKDRNLRY
                     QTATDLKTDLSRLRRDMDSGRKRAAEKADSRGGGTRQVEKSIAVLYFENLSGVKEDEY
                     FRDGITEDIITELSKIKGLNIFSRPTVLAYRDKPVTPAQIGQQLNAAYVLAGSLRRAG
                     TRLRINAQLVDTKTDFPLWSERYDRQMEDVFELQDEIVRKIAEALRVTLSPQEEEALA
                     SKPTENLQAYDLYLRGKSYARRLTRQDFEFALQMFENAVALDPEFALAHAAIANVCAQ
                     THYSYGRDPVWLERARAASRRAVALKPFLPEPLVAQSWILYADGKYDEAARGVRQAIE
                     RKNDCEGAYYLLGRALFASGHYQEIADVAEKAMEAGGQDYNIYVPIQNALGALGKTEA
                     VKNVRQRSIQALENHIRSVPEDARARVLLASDYAESGRGEDAIREANLAVALRPNEAT
                     VLYNAACVFCGLKDKAQAMDAMQKAWNAGFRDSDWARRDPDLGILHGDAEFERLYPEK
                     PAGA"
     gene            complement(20362..22470)
                     /locus_tag="PLB01_01660"
     CDS             complement(20362..22470)
                     /locus_tag="PLB01_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ76034.1"
                     /translation="MKPLFAFALALAASFAPAAGAQAPRVPTPSASAQMVVSGSKTPE
                     DTLDVPGSVEVVTGEELRRTGARTLQDALQNVVGVEAGGGSDGGPRFSNLGMWGLKEF
                     DALLVTVDGVPAGGPFNPNLAQIPIEDIERIEIAKGPQGTLYGVSAFAGMVQVFTRRG
                     KNTSVTLGLGSWNDKHASLAWEKHDGNSITHVAGSMFRDNGFQDNTGSSVDRLSIGFG
                     GKTREGSWDLTLAVLRDTAYWGSPLPIAGGEFVPGFEVDRNYAVGGARMDHRVYSLTS
                     TLVRRLSAQVQVENTLGLARDEQIAVRSFIDGVDGNTATANGTSLRPLQTTFFEDARV
                     VTTAGSHRLVTGIALTSGKTTVEGTGFDLTLTTGSNPVVPSVDSLPVGDTRSAEDKRT
                     FWGFYANDTWTPWKWLALTFGARYDRTSESLSAVVNEVGGPGPEAGSDERTDGNWSGG
                     VSALLRIVDGKAKGFLNVLHLYVAAKSNFKPAAPNILEAEATTILEPERSRSGELGLK
                     SRLFGGNVSLDMSLFHMTLENVVVSTLGPDGRPALTNAGEERFQGFEGQVTWAPPSWR
                     GFSLGAGYAHHDATYVSFSFVTPDGELRVVDGKRVELVPRDLWNVRLAWAPKSGLGFW
                     GAVRHQGIRPYTRRNTAYAPAFYEWDAGVSWENDWVRFSFTGRNLGDDRHVVTESELG
                     DAQFYLAPKRRYVGEMTIRF"
     gene            22498..23982
                     /locus_tag="PLB01_01665"
     CDS             22498..23982
                     /locus_tag="PLB01_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQQ76035.1"
                     /translation="MRIGIDFGTTHTVVALVDRGNYPVVAFEGGDFVPSLVAAHESSG
                     TLRWGWDAAAVRHEPGWTILPSVKRLLSDAGPSTEVAVGRRRLPIAALLEGFFSSLHR
                     ELVARSNAGIAANDSITAAVSVPANASTAQRFLTLEAFKSGGFAVEALLNEPSAAGFE
                     YAHRNRRDGRREHLVVYDLGGGTFDASLLRMTGKTNEVVATHGVQRLGGDDFDAAILA
                     LVLEKAHAHDVSDAERALVLEECVRAKESLSPNSRRFLVDLSALEKEPLVLPVDEVWE
                     VCVPLVARTLEAVGALVEDGGGVAWADVASLYVVGGAGLLPLVPRLLKERFGEKRVKR
                     SPHPFAATAMGLAVWLDREEGYALEDRLSRHFGVFREAGGGEDVAFDTIFEKGTRLPA
                     PGEAPVVVTRRYRAAHDVGHFRFLECSRLTDGRPDGDVTPWDEAYFPFDPALRSSDLA
                     AAPVRRREDGPEVAETYALGSEGAVTVTVTDAKDGFARTFRLTR"
     gene            complement(23933..25888)
                     /locus_tag="PLB01_01670"
     CDS             complement(23933..25888)
                     /locus_tag="PLB01_01670"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQQ76036.1"
                     /translation="MKLSRTFKVSGLLAAAGLLAVTLARNETPWAGLGIVLLVFGGAV
                     FCVSATRALLRGLLWRVGSRLVVSYLLLMSAVVFAAFFVYAGLLVVAGQLGTRRVEAA
                     LAKRNAGLLSRARDLALRGPSAPAGVAPRPWMKERGVYLVKQGSEIYEVAVVPRPDGP
                     FLLTERLDSGLRRDLERETGNVIRLLGAVKVREEVVDEKKGTVRFSFDEGGRKKKSVS
                     LSQEADSGGEGETGGDTAAPPAGSGPWSGRWSAFPLFLREPVHDAETGQPLEKQPVLF
                     LVRTSLEREAQELFRDTTVSGSEHVPMSRVVLAVVGGLGIATLVVFFLASFVAGLLVY
                     RIARATRRLSTGFGEIEKGNFGHRALLKGHDQLAALVESFNHMAERLAASVAARAEKE
                     ALERELEVARDLQRRLLPPAGFSFPGVSIAVDFRPAAAIGGDFYDLSGDARTLTVVVA
                     DVSGHGLPTGIVMAASKAALSALAKTGASGARLMELLDVEVVRSTDPRTFVTLAHLAF
                     DFGTATVSFTNAGHPYPYRVRPDGTVEALVNAARPLGVALPPGWQTVTAPLLPGDLWV
                     VYSDGLVEAAAEEGGEPWGFSRLEQCLKDGAGGSAAALRDRILASQRAHTGHDETDDD
                     RTLLVLRIEETVSASGETSAQNRPSHP"
     gene            complement(25885..27531)
                     /locus_tag="PLB01_01675"
     CDS             complement(25885..27531)
                     /locus_tag="PLB01_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQQ76037.1"
                     /translation="MAVPNRPTLAAFALGAALFAAGPLAAQIPGLSPTPTPAAAEAPG
                     DPYKRETPYGAFLGFMRASARENWTAAAEYLQWPKGAKTSREEIARELKAVVDERFTG
                     DLEKLSRSPLSSVDDGLGPDYERAGTVHRGDETFDVLLVRTRPAEGPAIWLVSSQTLR
                     EIPVAFQDLSVPELDRIMPAPLRRQVGGSVRLWQILAFLLLIPAAWLLARLLVYGAAR
                     LIDRTASRRQAFADFRHGLASFRAPLALLLAIPLHAFFVDRIGLPLLGRYAYARGVRL
                     ALVFTVGWLFVQIVGFLTSRATMRLLASGQAATSSLTIGRRVLQGIVVLGTAIAALGV
                     LGVNLTATLAGLGIGGIAVAFAAQKSLENLFGGVVVLTDKILRVGDVVKVGTVQGEVE
                     DVTLYATRIRTFERTVVSIPNGMMMTSQIENLSRRDKFLFRHTLGLNFETTAAQMQAV
                     LEACRARLAADPRVEPATARVRFLKLNAYTLDVEVFAYVLAPDWAAYLAVQEELLLAL
                     MHAVEGAGSGFAFPTQTTYLASEARALEPGAPQAAPSAPR"
     gene            complement(27545..28090)
                     /locus_tag="PLB01_01680"
     CDS             complement(27545..28090)
                     /locus_tag="PLB01_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQQ76038.1"
                     /translation="MSGYCRVAPGDPLHGPYHDTEYGFPLTDDAGLLERLALEIAQAG
                     LSWATILRKREAFHRAFEGFDPEVVARYGARQRRRLLADAGIVRNRLKVDAVIHNAGV
                     VLALRETHGSFDGWLAAHHPLPKDEWVKLFRSTFRFTGGEIVNEFLMSTGWLPGAHAP
                     ACPVARKIARARPPWMRAAVR"
     gene            complement(28090..29526)
                     /locus_tag="PLB01_01685"
     CDS             complement(28090..29526)
                     /locus_tag="PLB01_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HQQ76039.1"
                     /translation="MNVRVRVQKGAVIDGFVVGDLLHTGGMATLRKVSRPGISMPLLM
                     KVPAMLEGEDPAAIVGFEMEQMILPRLSGIHVPKFVASGDFAVQPYIVMEHIEGRTLL
                     TRLDDLPLPYPEVARIGNKVAAALLDLHHQNVVHLDIKPSNIIMRESGEAALVDFGLA
                     HHDQLPDLMGEEFRVPYGTAPYMAPEQILGNRTDPRSDIFALGVLLYFFSTGVRPFGD
                     PKGARALKRRIWWDPVPPRRLKDDYPPWLQEVVLRCLEVDPLRRYPTAAYVAFDLAHP
                     DQVKLTARSEKMERDPWSARVRRRFNPEVRPTALREIAPPKADTPIVMAAVDLDGDEE
                     LLEALRVTVRRLIEIVPGARFAILNVLKQRRIGIDTTLDEAGRNKHVERLVELKHWAR
                     PLAIGETRLTFHVLEAKDPAEAILEYVEANHVDHVVIGARTDSVRRSILGSVSAEVAS
                     KADCSVTVVRPPRRPEPPASAMDGPDPF"
     gene            complement(29531..30274)
                     /locus_tag="PLB01_01690"
     CDS             complement(29531..30274)
                     /locus_tag="PLB01_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQQ76040.1"
                     /translation="MRLALMADIHANREAFEACLADAAARRAERLVLLGDYVNYGADP
                     DWVVGAIMDRVAKGLAVALRGNHDAAVAGRNPTMDSSAESALEWTRNQLGADQRAFLA
                     ALPLTVEEGDCLFVHADASAPGRWTYVTDAADAARSLKATHRRVTFCGHVHRPAVYNM
                     SETLKLITFRPVPGVAIPLLPQRRWLAVMGSVGQPRDGNPAACWALVDTARAEITYHR
                     VPYEVDEAAKKIRRAALPEMYAERLTWGQ"
     gene            complement(30315..30391)
                     /locus_tag="PLB01_01695"
     tRNA            complement(30315..30391)
                     /locus_tag="PLB01_01695"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30355..30357),aa:Glu,seq:ctc)
     gene            30497..31573
                     /gene="ychF"
                     /locus_tag="PLB01_01700"
     CDS             30497..31573
                     /gene="ychF"
                     /locus_tag="PLB01_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQQ76041.1"
                     /translation="MEFGILGLELSGKTTIFSLLTGHATAGTPHKREAHVGIAHVPDA
                     RLDKLTAIFKPKKHTPATVKFVDVPGVAKGGAQSLNLPELRQMDGLAVVVRGFESDAV
                     PHPEGSVDPARDLELLETEMLLADLSVVTNRLERVEKDIAKKKTPELQTEKDLLVRFK
                     AALDRGEPLRGFDVHVEEEKYVKGFGLLTRKPMLVILNADESDAADLAGALAKAKLDA
                     WKGKPHVAFSAVSAPIEQEIAAMSPEDQAEFLKGLGLPDRALDRLLRAAYDLLGLMSF
                     LTAGEDECRAWSIPKGTHAAKAAAAIHTDFEKHFIRAEVVPFETLVEAGGSLAHCKTH
                     GTLRVEGRDYVVQDGDVINFRVSA"
     gene            complement(31649..33025)
                     /locus_tag="PLB01_01705"
     CDS             complement(31649..33025)
                     /locus_tag="PLB01_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ76042.1"
                     /translation="MDSKLLAVIKREYLQQVRGKAFWVATFLIPTLGLGMIFIQVALT
                     KTLVAKGRIAVVDLSGRLYEPLVAEYKARPSDEEAEKPKKDEKAPTTKVDEKSVTEKM
                     NESRSQKILTQLDLFKVDATPETLPDVRKRLNKDVQSEKIKAYIVLTPRTLETGHAEW
                     RAQSVKAEVVMREQIAGLLSRAATKERLKDKGVDPKVYEAARLRVDLEPHEAKEVESG
                     ESGKNVGMNLAISATFFFLIYISIFVYGAYIMRGVLEEKNNRIVEVIVSSIRPTTLML
                     GKILGIGLVGLTQYAVWAALALAITLPGVAALAGMGEGLPHIPVATIGAFVVFFVLGY
                     FLYASLYAALSAPFNTEQEAQQFVMIPGMMLILTSTTWFFAFNQPNGRLATILSFFPF
                     TAPLMMFMRISVQTPPLWQIATSVTLLVATIVLVAWFAGRVYRVGILMYGKKPTLPEI
                     FRWALRAD"
     gene            complement(33028..33813)
                     /locus_tag="PLB01_01710"
     CDS             complement(33028..33813)
                     /locus_tag="PLB01_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ76043.1"
                     /translation="MTAAISLHGVVKRFGKFTAVNKLDLEIPRGVTFGLLGPNGAGKT
                     TTIRMIMNITKPDEGEIRVLGEVMNEKLQERIGYLPEERGLYRKMKVIDQLLFFAAIK
                     SVDKGTAMKRLSPWIDSMDLRPWLQKKTEELSKGMQQKVQFLGTIVHDPEILILDEPF
                     SGLDPINVVLVKDFLTAFKKQGKTVVFSTHVLEQAERLCERIALLYRAQKLLDGTVKD
                     IKHSYRDRVPLPPPVEPEPELENIFIKCVQESGLVAPTAEELK"
     gene            33969..34046
                     /locus_tag="PLB01_01715"
     tRNA            33969..34046
                     /locus_tag="PLB01_01715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34004..34006,aa:Arg,seq:acg)
     gene            34106..34480
                     /locus_tag="PLB01_01720"
     CDS             34106..34480
                     /locus_tag="PLB01_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76044.1"
                     /translation="MRRIAFVAATLVLFAVSTAPSLAQEARPPEGARDETAREMAVLK
                     REVDRLKTEVTAARRQIEELKKAQADVNVHVEMLLKHRHQLFLDFMRADAFFPQAKGT
                     FFVTTPSPDTLTKTSGPVLGPK"
     gene            34506..35069
                     /locus_tag="PLB01_01725"
     CDS             34506..35069
                     /locus_tag="PLB01_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76045.1"
                     /translation="MNGSLRSTLLLGSVLLLAPGCSSGPVEVKAAPDARADAACPSGA
                     GARLTGTSFARQRNGKLVPGGGRPVYLDPATRYSAAVFRAIVEHQNKKSYFSAEKESG
                     TVVPDPAMFKCRRTVQADAEGKFTFADVPPGSYFVSSYVSWIKPDGTGEWSGVWNLSP
                     VVISADGKDVNVVLSGGPETIADPPAR"
     gene            35114..35563
                     /gene="tadA"
                     /locus_tag="PLB01_01730"
     CDS             35114..35563
                     /gene="tadA"
                     /locus_tag="PLB01_01730"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HQQ76046.1"
                     /translation="MRLALMAARRAPGHADVPVGAVVVKDGAVIARAWNAREKGADPT
                     AHAEILAIRKAAKKLGSWRLSGCTLYVTLEPCAMCAGAIVLSRLPHLVYGASDPKAGF
                     VGSLGDLCRDPRLNHRPAVTAGVLADECGRILVEFFREKRRTARAAR"
     gene            35529..36287
                     /locus_tag="PLB01_01735"
     CDS             35529..36287
                     /locus_tag="PLB01_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HQQ76047.1"
                     /translation="MKNAGPPAPPAENGFGAPEAPALRCPSCGAAAREGDRACAHCGS
                     LLATRRCVACFALSPRDAEKCVRCGALLPAEALNAVPGTCPDCKAGFVARQAGVAGFS
                     ECPRCGGLFLARASFDAVVKDAATREQARALDAGPERAAPEHGFHYRPCPVCRKFMNR
                     SNFGGGSGVIVDVCGHHGAFLDRGELTRIVDFVEKGGWERVKKREILRLEEEAAALES
                     RKRVAAGLGQALDAPPRGGGLAEVLSFLGSILRS"
     assembly_gap    36295..36312
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            36375..38105
                     /locus_tag="PLB01_01740"
     CDS             36375..38105
                     /locus_tag="PLB01_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76048.1"
                     /translation="MKQVVAALSILALCLLTPALSADTPDQPPSRDLDSSDRAGDRYA
                     LSMIRAGHATGRPWPEIARLSWDNPARFAEIVLPPEGPRVLREEMPADYVRAIEAEAA
                     RDEAYALGEARLWREGWLRIARDVVPQLDSKDAPGARRIALEFLKLENAGDGLGFDWR
                     EEPDTPGNRAARARWKALLEAPRFAASGTFRHVVADVVRMRAAPSPSAPVTARLRINT
                     WVRVARTDGEYVEVVVRAGGSPARGFVERRFVAEQSRLDVDPVLETARKEAAAGRPLE
                     ALTWAERAFALDRSSEAALDLLEKTARDAGQPERAEPARRLRTGQVPITFGVCARAGM
                     DTDFFQLVVVANLSPKGELSPVSPGDGARLASEAWFVVRPSVPGARGEPFYGTPTVRP
                     RRLSYMYGEGTLLSLGEACKPGEVWATVPLREVRRSGLNAAALEGKLETFLKEGFSDD
                     VRGSVVLDAVEAPTLLDGAPAESVLVRGRVRRARTDSEPGTYPVSQWALFGAGGRMLQ
                     RVGDVSFGATDVGEVLWTAPAFGPAWRLGFVLTDSSAGTTANGDVLVVLVDPEGNVKT
                     LPVHLWDGGC"
BASE COUNT         5640 a        13272 c        13614 g         5674 t
ORIGIN      
        1 acgccgtcgc gcgcggcgcc gtcgcggcgc tgggcgcggc cccggcgccc gagaacctct
       61 ccgtgccgta cctcgcggtg gagaacccac gccgcgcggc gggcctgatg gccgcgctcc
      121 tcgcgggccg gccggcgtcc cgcctcgtga tggcgggcgt cacggggacg agcggcaaga
      181 cgacgacggc gttcctcgtg gaccgcgttc tcgccgcgcg ccacgagagg cgcggcctct
      241 tcgggacgat cgtctaccga ggcgccggcg gggatgcggc ggcggtggcc tcgccgcaca
      301 cgacgccgga agccacggag ctccagccca tgctcggcgc gctcgtggcg gacggaggca
      361 cggcggcgac gctcgagtgc tcctcccacg cgctcgtcct cgagcgcctc gcgggctcga
      421 cgttcgacgt tgcgctcttc ctgaatctct cgcacgaaca cctcgacttc cacgccgaga
      481 tggacgacta cttcgaggcg aaggcgaggc tgttcgcgct gctgaagccc gagggacgcg
      541 ccgtcgtgaa tctcggggat gagtacggaa gacgcctcac gagtcgactt gtcgcaggtc
      601 ggaccgttgg tttcttctta gaaggtgaag aaggggcgga gcagacgcgg ccggcggccc
      661 tcgcgggtgc ggtcgcggtt gctgcggccg tggcgggcgg tgccgaaacg ggcggcctct
      721 tcgcgaccgt tctcgggcgc gcgacgctcg gactcgccgg aacccgcctc gagatcgagg
      781 tgaagacccc attcaccttc aaagaaaatc ttctcttcag catcaattcc cctcttctcg
      841 gaagaccgaa cgccgagaac ctgctcgccg cggcggccgc cggcatcgcg ctgggcttca
      901 cgcccgacga gatcgcagcg tctctctcgt cggtctccgt cgtccccggc cgcctcgagc
      961 gcatcgagaa cgatcgcggg ctgacggtcc tcgtcgacta cgcgcacaag ccggccgccc
     1021 tggagggcgt gctccggacc gtccgcccgc tcgcgcaggc gggcggcggc cgcgtgcacg
     1081 tcgtcttcgg ctgcggaggc gaccgcgatc gggccaagcg ccccgtgatg ggccgcatcg
     1141 cggcgcagct cgctgacgac gtcgtcgtca cgtcggacaa cccgcgctcg gaggacgccg
     1201 acgcgatcat cgcggaagtc ctgggcggcg tccccgccgg cgccgacgtc gcggcggtcg
     1261 ccgaccgacg caacgcgatc gccgaggccc tccgccacgc gcgcccgggc gacgtcgtcc
     1321 tcgtcgcggg caagggccac gagaccgaac aggtgatcgc gggcgtcgcg cacccgttcg
     1381 acgaccgcgc cgtcctgcgc gcgctgctgg cgggggaggc gggccgatga tcccgtggct
     1441 cctccacccg ttcttcggga aggtcccggt tctcggcgtc ttccggtaca tcacgttccg
     1501 cgccgcgaac gcggcgctca tggcgctgct cctcgccgtg ttcctcgggc ccagtctcat
     1561 ccgctggctg cgttcgaagc agatcggcca gtccatccgt gaggaagggc cgaaagccca
     1621 ccaggccaag gcgggtacgc cgacgatggg cggcgtcctc atcaacctcg cgatcgtcgt
     1681 cccgacgctt ctcttcgcgg atctgacgaa ccgctacgtc tgggtcgcgc tcggcgcgac
     1741 cgtcctcgcg atggtgatcg gcatcggcga cgactggcga aaggtgtcgc ggaaacagaa
     1801 tcgcgggctc acgggccgcc agaagctcgt cctgcaggcg gtgatcggcc tcgcggtcgg
     1861 ctgggcggcc gtgaacgcct ttgacggacg cgtcgatgcg acgcacgtcg cgttcccgtt
     1921 cctcaagaac gtccacccgg acctcggcgc cctctacgtg ctctttgccg cggtcgtcgt
     1981 cgtcgggtcc tcgaacgccg tgaacctcac ggacggcctc gacggactcg cgatcggcgc
     2041 gacgctgatc gcgtcgggca cgttcgcgat cctcgcgtac atcgcgggca acgcgcgggc
     2101 ggcgacctac ctcctcgttc cgtacgtgcc cggcacgggg gaactcgcgg tcttctgcgc
     2161 ggcgatcgtc ggcgcctcgc tgggctttct ctggttcaac gcgcatccgg ccgaggtctt
     2221 catgggcgac acgggctccc tcgcgctcgg cgccgcgctc gggaccgtcg ccgttctcat
     2281 caagcaggag atcctcctcg tcctcgtcgg cggcctcttc gtcgtggagg cggtgtccgt
     2341 catcctgcag gtgggctcct tcaagctcac gggccggcgc ctcttccgga tggcgccgct
     2401 ccaccaccac ttcgagctgc tcggctggcc ggagtcgaag gtcatcatcc gtttctggat
     2461 cgtcgcgatc ctcttcgcgc tcgtggcgct cgggtcgctg aagctgcgat gagcgtcgac
     2521 gtcggccccg acctgcccga gatccgcgct gcggcggtct tcggcctggc gcgctcgggc
     2581 cgggccgccg tgacggcgct cgccgaacgc ggcgtcgcgg tcaccggcac ggacgcgaag
     2641 gacgcgtccg agctgggcga gctgcccgcg ctcgacggcg tgcgctggga gctcggcggc
     2701 caccctcttt cgctcctcga cggcgtagac ctcgtcgtcg tctcgcccgg gatcccgctc
     2761 acgctgccgg tcttcgacgc cgcccgggcg cgggcgattc ccgtggtctc cgaggtcgag
     2821 ctcgcctcgc gactcctgcc gggcgtcgtc gtcgggatca cgggcacgaa cggaaagtcc
     2881 acgacgaccg cgctcgcggc ggccctcctc aaggccgcgg gccacgcggc gatcgcgtgc
     2941 ggaaactacg ggaccccctg gatctcgttc gcgacgaagg cgggcgcgaa cggcaccggg
     3001 tccgcgccga agacgtgggt cgtcgagctt tcgtccttcc agctcgaggg gatccggcgc
     3061 ttcgcacccg acgtggccgt ccacctgaac ctcacgccgg accacctcga ccggtaccgt
     3121 tcgatggacg actatggagc cgcgaaggca cggatcttcg agaaccagcg cgaggcgcag
     3181 gtcgccgtcc tgaacgccga cgacccgctc gtggcgcgga tccggccgcg cgcccggcgc
     3241 ttcgagttct cgagggccgc gcagccggcg ctcgggacgt ggctcaagga cgacctcttc
     3301 gtcgcggacg tcacgggcaa gggcccgcgc gtcatcgcga ggcgctcgga cctcgccctc
     3361 ccgggcgcgc acaacgtcga gaacgcgctc gcggcgctcg cggcgacgct tccgctgggc
     3421 gtcacgcccg agtcggcggt cgagacgttc cgcggtttcc gcggccttcc gcaccgcacc
     3481 gtgctcgtgc ggacgctcgg cggcgtcacg tggtggaacg actcgaaggg cacgaacgtc
     3541 gacgcgacgc tcaagagcct cgaggggttc gacgacgcgc gcgtgcacct catcctgggc
     3601 ggcaaggaca agggcgacga cttcggccgg cttgcgccgc tcgcggcgag aaaagcccgg
     3661 acgattctcg cgatcggcaa ggcggctccc gcgatcgcga gagcgctcgc gcccgtgccg
     3721 ggcctggacg tcgtcctcgc cggcacgctc gagagggccg tggaggaagc cgcgcgccgc
     3781 gcgggcgccg gcgacgccgt gcttctctcg cccgcgtgcg cgtccttcga ccagttccgc
     3841 aacttcgagc accgcggaga cgcgttcgag gaactcgtcc gcgcgctgcc cgagctcgcg
     3901 gggaaggtgg gctgatggcc aagaagctgg cctccgaccg cctcctgttc ggctcggtcc
     3961 tcctcctcgt ggggacgggc ctcctcatga tctacagcgc gtcgtcgctg caggccactc
     4021 tgccgtcctc gtccgctccg gccggcccgt acttctttgt cctcaagcag acgatcgccg
     4081 tcgcggtcgg gtcgcttctc gcgttcgcgg cgatgtccat cgactaccgg cgcctcgacg
     4141 acccgcgtct cgtcggcgtc atcctcgcgg ttctcgcggc tgccctcgtc gcggtgctct
     4201 tccgcgcgcc cgtgaacgga gcgcgccggt ggctcgcgct cggattcatg tccgtgcagc
     4261 cgtcggagtt catgaaggtg ggcctcgtga tcgcgctcgc ggccctcctc tcgcgccgcg
     4321 agggcgagat gcacctgccg acgcggaccc tgcttcccgt gctcggcctc gtcgtcgtgc
     4381 ccgcgggcct cgtcctcctc gagccggact tcgggacggc cttcacgtac ttcatcgtcg
     4441 cgggcgcgat gctgttcttc gcgggtctcc cgttccggtg gttcctcggc tcggcgctcc
     4501 tcctcgtccc gacgctctcg gcatgggtcc tgtccgccca gtaccgccgc gaccgcctca
     4561 ccgcgttcct ccacccggag atggacccgc tcggcaaggg cttccacgcg atccagtccc
     4621 tcatcgcggt cgggacaggc ggcgtcacgg gcctcggact cggcgagggc aagcagaagc
     4681 tgttcttcct gccgtacccg tacacggatt tcatcttcgc gatcgtcggc gaggagctcg
     4741 gtctcgtcgg ctgcgtcgcc gtgctcgcgc tcttcggcct cgtcttcgcc cgcggcgtgc
     4801 gcgccgcggt gaacgcaccc gactcgttcg ggcgcctcct cgggatcggc ctgtccgtga
     4861 tgatcgtcgt ccaggccctc atcaacatct ccgtcgtcct ctcgctcgtg cccaccaagg
     4921 ggatcccgct gccgttcctc tcttacggcg ggtcggcgct ctgggccgac ctcctcgcgg
     4981 tcgggatcct cctcaacctc tcccagcacg cctcatgacg agcgccgcga gctcaccggc
     5041 cacgggcggc ttcctgatcg cgggcggcgg gaccggcggc cacgtctttc ccgcgctcgc
     5101 cctcgcgggc gagatccgcc ggcggcggcc cgacgcgccg atcgtcttcg tcgggacgcg
     5161 gcgcggcctc gaggccgacc tcgtcccgcg cgcgggatgg ccgctggagt tcgtgagggc
     5221 gaagggcctc gtcggcaagg gcgcgagggc gcgggtcgag ggtctcctcg cgctcccgct
     5281 cgcgttcgcc gactcgatcg ggatcctcgg ccgccacgcg ccgcggacgg tcgtcggcgt
     5341 cggcggctac gcgtccgggc ctctcgtcgc caccgcgtgg gcgcggcgca tcccgaccgt
     5401 catccacgag cagaacgcgg tcccgggact cacgaaccgg ctcctctcga aggtcgcgac
     5461 gcgcgtcgcg acggggacgg acgcgggcgc ggcgcgcctc ggcggcacat gcgtcgggaa
     5521 ccccgtgaga ggggagttct tcgaggtttc ctcgctcggg gggcgcgaga cgccgcgccg
     5581 gatgcgcgtg ctcgtggtgg ggggcagcca gggcgcctcg atcctgaacc gcctcgtccc
     5641 gggcgcgctc gcgcgcgtgg acgcagccgg agtgcgattc gcggcgatcc accagaccgg
     5701 aagaggccgc gcggccgacg tcgccgctgc gtacggaagg cacgcgttct ccgtctcggc
     5761 cgtcttgccc gtcgaattcg tggaggacat ggcgcaggcg tatgccgagg cggacctcct
     5821 cgtcgcgcgt gcgggagcga tgacggtcgc cgagatcgcg gcctccggcc gtccggccgt
     5881 cctcgtcccg ttcgcgggtg cgacgcacgg ccaccaggaa gcgaacgcgc gcgcgctcga
     5941 gcgcgacggc gcggccgtcg tcctgacgga agacgagtcc tccgaggaga gcctcggaaa
     6001 cgcgatcgcc gcactcctct cggatcccgc gcggctcgcg gcgatgggcg acgcggcgcg
     6061 ccgctcggcg cggccggacg cagcggcgcg gctgtgcgat gtcgttttcg aagcggaagt
     6121 gagccggata tgaatttcct tcgcgtgaag cgcctgcatt tcgtcgggat cggcggcatc
     6181 ggcatgtccg gcctcgccga gctcctcaag agcgtgggcc tcgaagtgac gggctcggac
     6241 ctctccgagg gcgagcaggt gctccggctc cggacgctcg ggatcccggt cttcctcggc
     6301 caccacgcgg agaacgtgca gggcgccgac gtcgtcgtct actcgagcgc cgtccacgac
     6361 tcgaaccccg aggtcgcgac cgcacgcagc atgggcatcc ccgtcatcaa gcgggccgag
     6421 atgctcgccg aggtcatgcg cgtcaagcgc gggatcgcga tcgccggaac gcacggcaag
     6481 acgacgacga cctcgatgac cggcgcgatc ctcatggaag ccggcctcga tccgacgatc
     6541 atcgtcggcg gacggatgcg cgaagtcggg acggcgcgcc tcggtcgcgg cgagtatctc
     6601 gtcgccgagg cggacgagtt cgaccgctct ttcctcgcgc tctcgccgat gctcgcactc
     6661 atcaacaaca tcgacctcga gcacctcgac acctaccgcg acctgcagga cctgcaggag
     6721 acgttcgcgc gcttcgcgcg cagcgtcccg ttcttcggag ccgcgatcct cggcctcgac
     6781 gacccgcacg tgcaggaaat ccgcccgctc gtctcgcggc gcgtcgtcac gtacgggctg
     6841 acgccccagg cggacctcac ggcgcgcgac gtcgtcctcg agcgcacggg ctcgcgtttc
     6901 gtcgcgtttg cggaccgcga gttcacgggc gccgtgtcgt tgcaggtggc gggtcttcac
     6961 aacatcaaga acgccctcgg cgcactcgcc gtcgcgcggg agctctcgat cccgttcgcg
     7021 acggccgcgc gcgctctcgc ggagttcaag ggcgtcatcc ggcgtttcga gaagaagggc
     7081 gaacgcgcag gtgtggtcgt catcgacgac tacgcgcacc acccgaccga agtcgccgcc
     7141 acgctcgccg cagcccgcca gtcctatccc gaccggcgga tcgtcgcgct cttccagccg
     7201 cacctctact cgcgcacgaa ggacatggcg caccagttcg gcgccgcgtt cctgaacgcc
     7261 gacgtcctgc tcgtgacgcc cgtctacggc tcacgcgagg cgccgcggga gggcgtcacg
     7321 ggcgcgctcg tcgcggacgc cgcgacggcg cgcggccacc gcgccgcccg cttcatcgcg
     7381 gaccgcaagg gcatcctcga cgccctcgag tgggagctca aggacggcga cctcctcatc
     7441 acgatgggcg cgggcgacgt gctgcacttt ggagaggact acctgaagaa agagggcggc
     7501 gtcctggagc ggagcggcaa gctcacggct ccggcacccg ccgcgcatcc gcaaccaaca
     7561 ccttctaaga aatccgaatc cgaaaacaag gggtccgaca agagttgagc tcgttttatc
     7621 gccccccgtc cgcccggacc gtgcgcccca tgcggcgccg ggcggggagg cgtctgctcg
     7681 tcccggcgct cacgctcctc ttcctctccg gtctcgcgct ctcggggctc gtgatccttc
     7741 gcacccatcc gcgctttgcc gtgacgcgcg tcgtcctcga gggcgtgccc gaggccctgc
     7801 gcgccgaggc cgaagagctc acggacgggt ggatcgggcg gccggcgctc ttcgtcgacc
     7861 tccagggacc ggtcgaggag ctctcgaagc gctcgtgggt cgcctccgca tccgcgaggc
     7921 gcgtggtgcc tggcacgatc gtcgtcgtcg tcgtcgcgcg gccccccgtc gcggtggcca
     7981 tccgggagga ccggggcggc gagctctgga cgctggaccg cacgggcagc ttcatcggcc
     8041 cctacggcgg gcgcgccctc ggacgcgaag acgacttcgt cgtgctctcg ggcgcgagcg
     8101 acgggaccgc ccgcgcgcgg ggcgccgcct tcctcgacgt cctcaagacg gaggaccccg
     8161 accttctcgc gcgcgcgtcc gaggtcgccg tcgtcccgga ggggttcgcc gtcacggacc
     8221 gcgtcgcgtc gtgccggctg ctcttcgggc ccgacgcggc ggcaccgggg cgcgcggcac
     8281 ccatctggcg ggcgtacctc gcgctcctcc ccgagctcga gcgcaacgca ttcccgactt
     8341 ccgacgcgga cctgcgtttc gcgggccgca tcgtcctgaa ggcgcccggc gacaccgggc
     8401 gcggaaagac ctaagggtta acggatgccg aaaacaacgc cctacctcgt cagcctcgac
     8461 atcggatcga cgaaggtgtg cgccctcatc gcggagtcgg acgcgaccgg gaggatcgac
     8521 atcatcggaa agggcaccgc cgcccacaag ggcgcacgca agggggcgat catcgacgtg
     8581 cccgccaccg tggacgcgat caaacgcgcg acggaggaag ccgagatcat ggcgggcgcg
     8641 cagatcgagc gcgcggtcgt gggcgtcgcg gggcccgccg tcaagtcgtt caacagccgg
     8701 caggtcgtcg cggttgccgc caagaaccgc gagatctcgc gcgaggacat ccagcgctcg
     8761 ctcgacgcgg cccgctccgt gccgatcccc tccgagttcg agatcctcca cgtcctcccg
     8821 cgcgagttcg tcgtcgacgg ccaggacggc gtcgcggacc cgctcggcat ggtcgggagc
     8881 cgtctcgagg cggacgtcca cgtcgtgacg gtccccgtcg cgacgacgca gaacctgctg
     8941 aattgcgtca accgggcggg cgtcgaggtc atcgagatgg tcctcgagcc gctcgcggcc
     9001 agcgaggccg tcctcacggc cgacgagcgc gagctgggcg tcgtgctcgc cgacatcggc
     9061 ggagggacga cggagatcgc cgtctggcgc cacgggacgc tcgcccatac ctccgtcctg
     9121 ccggtcgggg gcgaccattt cacgaacgac ctcgcggtcg tcctcaagac gccggtgccc
     9181 gacgccgagc gcctcaagcg gaagcacggc gccgctctcg cggccctcgt gaacgaatcc
     9241 gattcgatcg acgtgccgct caccgggggc cggggcgtcc acccggtcaa gagacgcgag
     9301 atcgccgaga tcctgcagcc gcgcgccgag gagctcctca cgctcctgtg ggaggaatcc
     9361 gtccgcgagg tgccggccgg cgagctgcgc gcgggcctcg tcctgacggg aggcggctcc
     9421 gagctcgacg gcctcctcga ggtcgccgag caggtcctcg gcgtcgcggt ccggcgcggg
     9481 gtgccgcgcg ggctcggcgg cctgacggac atcgtcgcgt cgcccgagtg ggcctgcgcc
     9541 gcggggctcc tgctctacgg gcgcggcgcc gcggccgcag cccggcggcg caaccggggc
     9601 gcgtcgcccg ggttcctcga caagatcaaa ggcagcttcc gaaacctgtt cccctcgtcc
     9661 accgctctgt gatatcgatg gaggaaagaa aatgatccta ttcgacgacg acgacgccgg
     9721 ctccaagcgc acgtcgcgga cgatcacgtt cagcgaggac tccgctcctc ccgcccgcat
     9781 caaagtcgtc ggcgtcggcg gcggcggcgg caacgccgtc aaccgcatga tccaggcggg
     9841 gatcaagggg atcgagttcg tcgcggcgaa cacggacctg caggtcctga aggtgaaccg
     9901 ggcggccacg aagctccagc tcgggatcac gacctcccgc ggcatgggcg cgggctcgaa
     9961 cccggacgtg gggcgcaacg ccgccctcga ggacgccgag aggatcacgg aggtcctcgc
    10021 gggatcggac atggtcttcg tgaccgccgg aatgggcggc gggacgggga cgggcgccgc
    10081 gcccgtcgtc gcgcggctcg cctccgaagc cggggcgctc gtggtcgcga tcgtcagcaa
    10141 gccgttcgcg ttcgaggggc ggaggaagct ccggtacgcc gaggacggca tcgccgagct
    10201 gcgcgagtac gtggacacgc tcatcacgat cccgaacgag cgcctctact cgttcgtgga
    10261 ccgcaacgtc acgacgtggg aggcgttccg catcgcggac gacgtcctgc gccaggccgt
    10321 ccaggggatc agcgacctca tcacggtgcc cggcatcgtg aacctcgact ttgcggacgt
    10381 gaagacggtc atggagaaca tgggcatggc gctcatgggc acgggcaccg cgacgggcga
    10441 gcaccgcgcc gtcgaggccg cgcagcgcgc gatctcgaac ccgctcctcg aggagcagtc
    10501 catccagggc gcccgcgcga tcctcatcaa cgtcaccggc ggcgaggacc tgacgcttgc
    10561 cgaggtcaac gaggcctgcg cgatcatcca gcaggccgcc gacgaggagg cgaacgtcat
    10621 cttcggcctc gtgcaggaca aggacatgaa ggacgaggtc aagatcagcg tgatcgcgac
    10681 cggtttcgac gcgcccgccg cactcgcctc gcgccgcgag gtctcgctcc cggtgaatcc
    10741 gtccggagcc cgcggcgccg cgccggagcg gccgcggccg ggcgccgagc ccgctatgcc
    10801 cccgactccc gaggaccagg gctacggggt gaacctcatc aacatgcggg atccgcgcga
    10861 ggacatcttc gacatcccga cgttcctgcg tcgccagatg gactgatgtc ttgcgggggg
    10921 ctcctacgcc gccccccgca cccctggcga ggcacgtttt gagacccgcg cgatgaggag
    10981 ccgaggggcc ttcaccggca tcctgctgac cctcttcctc gcgcgggtgg cgtccctcgt
    11041 cttccacttc gtcctcgccg cgccctccgg cgcgcgcgtc gccgagcacc cgctccagct
    11101 cctcccggcg gcgctcgcgg gcgagatcgg gaccctcgcg gcgctcggcg ctctcgcggg
    11161 cgtcctgtcg tggctctcca cccgggcggg gcttctgctc ctcgccggcg gcggcgtctt
    11221 cgtcctcttc ctctcgcagc tcgacctcga gctcgtgcgc tggacgggcg agcacctcaa
    11281 cccgcagtgg atcggcgcgt acaacctgct cgcgcaccgg cgtctcctcg tggacgtcgt
    11341 cttcggcgac acactcgcgc tcggcgcggc ggttctcctc gtcgcagttc cggccggcat
    11401 cgtcttcgcg ctcgcgagaa ggcggggacc ggagcgcgcc gcgcccctgt ccgtggcgtt
    11461 cctcgcggcc gtctcttgcg gcgcgctcac gttcgagagc gccctcgcgc ccacgaggct
    11521 cgccctgcgg cgcgcccggc cggtcctcgt cgggctcgtc tccgagtccg tcgcggcgct
    11581 cggggacgcc ccctcgccgc gggaggtccg cgcggggatc gcgtcgctgc accgcttcct
    11641 gaaccggccg ccgcggtggt ttcccgacgc gggctacccg gtctggcacc ccgcgccggc
    11701 cgaggcggcg tccgtggccg cgttccgcgc gcggccgctc gagaagaggc cggacgtcct
    11761 cctcgtggtc ctcgaatccg cgcgcggctg ggagacggac gtgcggcggc ccggggtgga
    11821 atcgcgcatc ccggagattc accggctctg gaaggagcgc ggcgtcgcgt tcccggtctg
    11881 catcgcctcg ggctatccct cggtggaggg gcgcggcggc atcctgctcg gggtcgcggg
    11941 ccacccgtcc ggcatccttc tctacaacgc gcgcgagctc aacgtccttt cgatcggcga
    12001 gctactcgcg aaagccggtt acgcgcgcga gctcgtggcg gcgacgagcc ccgcgttcga
    12061 gaagatggag tactggtacg cgcgctggtt cgacgccgtg cgctacgacg cggcggacac
    12121 gacggacgaa gacctggcgc ggcggacgat cgagcgcctc gaatcccggc gcgaggcgcc
    12181 gctcttcctc acgctcttca cgatcgcgac gcacccgccc gtctatcggc cgcccggctc
    12241 ggacccgaag acgccgcgcg agtcgtatct cgcggcgctt ggctacacgg accgcacgct
    12301 cggccggatc ttcgagagcc tgcggcggac gccgcggggg cgggacacgg tcgtcgtcgt
    12361 cgtcggcgat cactccacga cgaacccgtg gcagtcgatc cggctgccgc gcctcgggac
    12421 gccgaacgcg ggcgagaact ggacgacgct cgtcgtcgcg gcgccgggcc tgccggcggg
    12481 cacggttcgc gaggagctct cctcgcagct cgacgtcggg ccgacgctcc tcggcctcct
    12541 cggcctcgac gtctcgaacc acttcttcgg ccgcgacctc ttcgagaagc ccgccccggc
    12601 gcctcagggc gtcctcgcgc tgcgcctgcg cggcctcgcg gcgttcgagg gcccgtttct
    12661 cctgcagacg cggctcgacg acccgtcgtt cgcccagaag tggcgctggc aggactacga
    12721 ggcgtcgccg gacgacgaca acggcggata ccaccacggc acgctgcagg agctgaccgc
    12781 ggccgaccgc gcgcggatcg aagagctcaa gacgatggcg cgggcgtggg gcgccgtcct
    12841 cgacggcaac cgcgtcatgc cgccgggggc gacgcgctga ggcgctctcg aacgattcac
    12901 caaagcccaa gagagcaccc cgaaactcgc agcgggaatg tgggcgctaa ctcgttcggg
    12961 tagtctgcag gagcatccgc cttagcgccg ttggtgccac gccgaactct cgccgaacga
    13021 atcgagctaa ccccggcggg ctgaacccaa cgcgtgccgc gacgaccttc agagggatgt
    13081 tctgctgagt aaggttgaca atcgctgaag cccgccgcgc gattcgaacg tctttcatgg
    13141 cgagaccgta tgcgcgccag cgcctttgaa gagtgcgggt cgacagtccg gccctctttg
    13201 ccgaggcctt cagcgtccca cattccagat agaggtccag ccagttcatt ggtgacccat
    13261 gtggtaacac gccacggtcg ttcgccatgc ccgcaactcc acgccgatgg cctgaaatga
    13321 taacaaccgg cccttattga cctgccggcg gacgggtagt ttcgtggaca agctgtgatt
    13381 cccgttcgcg tgggtagcaa ctctgaccgg cccgggaaac tttcccctga agtattgtcg
    13441 aagccgtgtc gtgtcaaccg ggctctttcg attcaggagg cgtgaacaat gcgcaagccc
    13501 cttcacgtag cgatgctcgt cggccttgca gtgtttctct cgatggcctc cgcgccgggg
    13561 cttcgtgcag aagccattgg cggcgggtgg gagtgcacgt tgtacgagaa cacactattc
    13621 aggaacggcg tagccgtcct cacgtggtac acgtatgagg actgtacgcc cgtgtccggt
    13681 acggacgtcg gcacgatcgg gagcggcgga ggaggatcag ccgatccctt ctccgactgc
    13741 aaggatacgt gtgacaagcc gtgtcgcgac tggaacaaca gctgccagcg gactgacgtt
    13801 tgcaacgggc ttgtgtcgtg caagtcgtgc tgctacatgt gggctgaggc ggtgagaaac
    13861 tcgcgaggat gtaacgtacg ctgtaaggct gaaacggaga gcgccaaggg cgcttgccag
    13921 ggagattgcg gcaaccactt caataactga cgtcgtcgag gaggcatcaa tgccgcccgc
    13981 acttttgttt agtctcctcc tggtggccga cgcgggaacg ttcgaccgcg cgccgaagga
    14041 ggcgtgggaa gttcgtaaca gctatgacct ctcggatcaa gccggacggg tcgtggtcaa
    14101 cgtgacttcg cgaagctact cgctgggcgc ggctgccagc ggtgattcga cattccgcgc
    14161 tcagacggag gtgacgttcg gccggtcgga cgggacaatt ttcatctgga gagagacgct
    14221 cgaaccactg agcggggacg cccgcggaag gatgacggtc acattggagt cactcgccga
    14281 tcattgcaag atcgagctcg acacgggtcc cgcgacacag tccggaacct acgacagaat
    14341 ccgccttcaa gtccctggtg cggccttggt gttcgccgag gtcgacgccg ggctcgtcga
    14401 gcgccagttg aagagcgccg acctcgaagc gttcagggag aaggtcccgc ccggcttaag
    14461 ggagatgtgt acttttctcg tcgagctgag tgacgtcatc ccggccccgg gcggcatcct
    14521 gagggactgg gtgacacgaa tcgccgcgcc ggcaggtaca aaggaggatc cgtcgcgggc
    14581 aaggaaatgg tcgcgcgaga atgaacagtc tcagacttcg aagcacgtgg tgccggttct
    14641 ggctacacct ccccctgact ccatccgggg tcccggcgac gagatcaaga agacgaaggt
    14701 cagtcgagag gaacggaggc cttccggcgc cgccgctccc gcagccacgc ccgggccttg
    14761 agccacgtcc gctcgaacct cgggttctgc tgtcgaaagc ggcggacctt gcgctggacc
    14821 cagcggctct cgatgtagag gatcgccaac cccgcgacgc cgaacggcca gccctggatg
    14881 acgggcaaga ggccgccgag gacgccgagg gcgagaagcg tccagccgag cgcgagaagc
    14941 gccatgcgca cgggccggct gcggcgccgc acgaggccgc gggaggggag gggagcgctc
    15001 aatcccgtcg cgggatgacg ccgcgttggg cgtctcgcgc gatgcgcttg gcctcgtcgg
    15061 ggaagttccc gtcgacgatc cagtcgaggt aggacgggtt tttttcggcg acgtccgcga
    15121 gccgcgtccc gcggtgctgg ccgaaggcga acacgacttc gccgtcgcgc cacaggaagc
    15181 gcttgtcggg gtcgacgtac cggccctcgg gcggccgcga gagttcgtgg agcgccgcga
    15241 cgtcgcgcgg gaggtccgca tagcgccgga gctgcccggc gaggacctcg gccgaggcga
    15301 cggcgtcggc gagcgcggcg tgggccgcgg catgctcgcg cccggcgaag aagcgcacgg
    15361 cggcgccgag gtcgcgcggc tcgcgccgga agtagatcgt ctgcatgtcg acgacgcgcc
    15421 tcccgtccat cgagaacggg accttcgcga gctcgaactc gcgcaggagc accgggacgt
    15481 cgaactgtcg gatgttgtag cccgcgaggt cgcagccctc gagaaacgcc gcgagctccg
    15541 gggcgagcgc ggggaacgcg gggcagcccg ccacgtccga gtcggagatg ccgtgcacgc
    15601 gcgtggactc gcgcgggatc ttcacgccgg gattcacgcg cttctcgagc gtggcgacct
    15661 cgccgccctt gcggaatcgg acgaccgcca cctccacgat gcggtccgag aggcgatccg
    15721 tgcccgtcgt ctcgaggtcg aacaccgcga gggggcgctc ggtgcacagc tcgaagacgg
    15781 ggccgagagc gtcgaggggg agagcggagt cggtcacgcc gggatcatgg cccaactcgc
    15841 ctcgcaccgg gcagggcggc ctcccacgtc cacgaaccac ccggcgcgcc gggcggacct
    15901 tcccagacga ggcgcgcggc gtgcaggacg ggtgcgggcg aatcgggcgt ccggaaggcg
    15961 tcgtagagcg ggtcgccgag gagcggcgtg ccgagatggg ccaggtggac gcgcagctgg
    16021 tgcatccggc cggtcccggg cgtcagctcg acgagcgccg tcgtcccacg gtcctgaacg
    16081 acgcggtagg cggtcctcgc ctcgcgtcct gccgggtggg cggcggtccg accgggcggc
    16141 gggacgggtg ggatggcgag cacgcgacgg cccgagagca ggtgccccgc gcgcgcgggc
    16201 ccgtaggccg ccttgagcgc ccgcttcagg agctcgggct tgcccgtgcg gtgctcaagg
    16261 agcttttccg tcacgacgcc ttcgggcggc gacggcacgc cgcgcgtgac ggcgagatac
    16321 gtcttcgtga cggacgtcgg ccacgcctcc agcgcggcgg agagcgcctc gggcgtcctc
    16381 gcgaggagga ggacaccgga cgtctccttg tcgaggcggt ggacgagggc gaggttgtcg
    16441 ccgaactccc tgcggagcgg cccgatgagg ccgtgcggcc cgtggaccgc gacgcccgcc
    16501 ggcttgtcga tcgcgaggta ggcctccgcg ccgcgcggcg acgggagtgg cttcaccgcc
    16561 ggaacttcag ggccgacccg atgacggcga gactcccccc gatgttcgcg gccatgccga
    16621 tcacgatcgg tgtctggtgg aaccggatcc cgtacaggag gtagacgagc tggccgccga
    16681 ggaagaggag atacgtcgtg atcgagacat cttccgaact ccttcgcttc cagatccgga
    16741 gggcctgtgg cacgtaggcc gcgccggtgg cgatgccgac caccgacacg gcccaggcga
    16801 ggaaggcgtc gaaccccgtg ggcgcgtcga taggcctatc tccggttccc gaagaggcgg
    16861 agcagcatca ggaagaggtt gatgaagtcg aggtagaggg cgagggcgcc cgagatcgcg
    16921 ccgcgcttgc cctcctccga cgcgccgtcc accgtcgccg ccatcatctt gagcttccgc
    16981 gtgtcgtacg ccgtgagcgc ggtgaagacg atgacgccga cgcaggagac gacgaacccg
    17041 agcatgtcgt tccggaggaa gatgttcacg acggacgcga ggatgacgcc gatgaggccc
    17101 atgaacgcga aggagccgag gccgtcgagc gcgcgcttcg tgacgaggcc gtagacgctc
    17161 atcgcaccga acatcccggc cgtgacgacg aacgtcgaga cgaccgaagc ggacgtgtac
    17221 gcgaggaaga cgaccgagag cgtgagcccg ttcaacgccg agtagaagag gaacacggcg
    17281 cccgccgcgc cggacgagag gcggccgacg agcccggaga gccacgcgac gagcgcgagc
    17341 tcgccgatca tgaggccgaa gaacaccatg cggttcccga agacgagctt gaggaggctc
    17401 tcgttcgaca gcgtcgcgaa cgcggcgccc gcggtgacga ggagccccgc ggccatccag
    17461 acgtagacgc gggcgaggaa cgacgggatc gcgaccgcag ccgagggatc gatcggccgg
    17521 ggggaatcga aggggcgcgg cggaagctcg gtgggcatct tgagatctcc tctcgaacgc
    17581 ccatcctaac gcgcgggcgt taagattcat tccctatgaa acgcaccgca tccgcactcc
    17641 tcgtcaccct ccttctcctc cctctttccc ttctcgccga cgccggcaag ttcggcgcgc
    17701 cgctcgggaa ctcgccgaag gtcgcgctcg ccgacctcgt gaaggatccc gccgcgtggt
    17761 ccgggaagac cgtgaagacc gagggcgtcg tgtcctccgt ctgccaggaa aagggctgct
    17821 ggatggtcct gaaatccggc gaccagtccg tccgcgtgcg gttcaaggac tacgcgttct
    17881 tcgtgccgaa ggactccgcc ggcgccacgg cggtcctcga gggcgtcttc accgtgaaga
    17941 cggtcccgga ggccaccgcg aagcactacg ccgaggagac gccgggcggc aagccggccg
    18001 acatcaaggg cgaccagaag gagctctcct tcctcgcctc cggcgtcgag ctctcgaagc
    18061 cggcgaagta accccgctcg tttcggattt ctctgggtta atctcgccca cacgtggtcg
    18121 agcagcaaga ttccctggtc gggaagagtg tgtcccacta ctccgtggtc cgccgcctcg
    18181 ggagcggcgg gatgggcgtc gtgtacgagg ccgaagactc ccgtctcggc cgcaacgtcg
    18241 ccctgaagtt ccttccgccc gagctcgcgc gcgacggctc gtccctcgag cgcttccagc
    18301 gcgaggcgcg cgccgcttcg gccctgaacc acccgggcat ctgcaccgtc tacgcgatcg
    18361 aggagcacga cggccagcac ttcatcgtga tggagtgcct cgagggacag acgctcgcgc
    18421 agacgatggc tgcgcggccg gcattcgacc tcgagagcgt gctttcggtc ggcatccaga
    18481 tcgccgacgc cctggagtcc gctcacgccc gaggcatcgt gcaccgcgac gtcaagccgg
    18541 cgaacgtctt cgtgaatgcc cgcgggcagg tgaagattct cgacttcggc ctcgcgcgga
    18601 tcgagggcgc tcgccgcggg aacgacggcg ccgtctctcg cctcgagacg gtcggcgacc
    18661 tcacgacgcc cgggaccgcg atgggcacgg tgtcgtacat gtcccccgag caggcgcgcg
    18721 gccagctcgc ggacgcgcgc acggacctgt tctcgctcgg gacggtcctc tacgagctcg
    18781 cgacgggcgc gctgccgttt ccgggggaga cgtcggcggt cgtcttcgac gcgatcctga
    18841 accgcgaccc gctgccggcc gcgcagcaga accccgcgct gccccccgag ctctcccgca
    18901 tcctcgacaa ggcgctcgag aaggaccgca acctccgcta ccagaccgcg acggacctca
    18961 agacggatct ctcgcgcctg aggcgcgaca tggactcggg aaggaagcgc gcggccgaga
    19021 aggccgactc gcgcggcggc ggcacgcggc aggtcgagaa gtccatcgcc gtcctctact
    19081 tcgagaacct gtcgggcgtg aaggaggacg agtacttccg cgacgggatc acggaagaca
    19141 tcatcacgga gctctcgaag atcaagggcc tgaacatctt ctcgcgcccc acggtcctcg
    19201 cgtaccgcga caagcccgtg acgcccgcgc agatcggcca gcagctcaac gcggcgtacg
    19261 tgctcgcggg aagcctgcgc cgggccggca cgcgcctgag gatcaacgcg cagctcgtcg
    19321 acacgaagac ggactttccg ctctggtccg agcggtacga ccgccagatg gaggacgtct
    19381 tcgagctgca ggacgagatc gtccggaaga tcgcagaggc gctccgggtc acgctctcac
    19441 cgcaggaaga ggaagccctt gcgagcaagc cgacggagaa cctgcaggcg tacgacctgt
    19501 acctccgcgg gaagagctac gcgaggcgcc tcacgcggca ggacttcgag ttcgcgctcc
    19561 agatgttcga gaacgcggtc gctctcgacc cggagttcgc gctcgcccac gcggcgatcg
    19621 cgaacgtctg cgcgcagacg cactacagct acggccgtga ccccgtctgg ctcgagcgcg
    19681 cccgcgccgc gtcgcgccgg gccgtggccc tcaagccgtt cctccccgag ccgctcgtcg
    19741 cccagtcgtg gatcctctac gcggacggca agtacgacga ggccgcgcgc ggtgtgcgcc
    19801 aggccatcga gcggaagaac gactgcgagg gggcgtacta cctcctcggc cgcgcgctgt
    19861 tcgcatccgg ccactaccag gagatcgccg acgtggccga gaaggcgatg gaggcgggcg
    19921 ggcaggacta caacatctac gttccgatcc agaacgcgct cggcgcgctc ggcaagaccg
    19981 aggccgtgaa gaacgtccgc cagcggtcga tccaggcgct cgagaaccac atccggtccg
    20041 tgccggagga cgcccgggcg cgcgtcctcc tcgcgagcga ctacgccgag tccggccgcg
    20101 gtgaggacgc cattcgggag gcgaacctcg cggtcgcgct gcgcccgaac gaggcgacgg
    20161 tcctctacaa cgcggcgtgc gtgttctgcg ggctgaagga caaggcgcag gcgatggacg
    20221 cgatgcagaa ggcctggaac gcgggtttcc gggactcgga ctgggcgcgc cgggacccgg
    20281 acctcgggat cctgcacggc gacgcggagt tcgagcgcct ctaccccgag aagcccgccg
    20341 gcgcctgaaa cgcgccccgg ctcagaacct gatcgtcatc tccccgacat agcgcctctt
    20401 cggcgcgagg tagaactgcg cgtcgccgag ctcgctctcg gtcacgacgt ggcggtcgtc
    20461 gccgaggttg cgccccgtga aggagaaccg cacccagtcg ttttcccacg agaccccggc
    20521 gtcccactcg tagaacgcgg gcgcgtacgc cgtgttgcgg cgcgtgtacg ggcggatgcc
    20581 ctggtggcgc accgcgcccc agaagccgag gcccgacttc ggcgcccacg cgaggcggac
    20641 gttccagagg tcgcgcggca cgagctcgac gcgcttgccg tccacgacgc gcagctcgcc
    20701 gtccggcgtc acgaaggaga agctcacgta cgtcgcgtcg tggtgtgcgt agccggcgcc
    20761 gagcgagaag cccctccacg agggcggggc ccacgtgacc tggccctcga agccctggaa
    20821 gcgctcctcg ccggcgttcg tgagggcggg ccggccgtcg ggcccgagcg tcgagacgac
    20881 gacgttctcg agcgtcatgt gaaacagaga catgtcgagc gacacgtttc cgccgaagag
    20941 gcgggacttg aggcccagtt cgcccgagcg cgagcgctcc ggctcgagga tcgtcgtagc
    21001 ctcggcctcg aggatgttcg gcgcggcggg cttgaagttg ctcttggccg cgacgtacag
    21061 gtggaggacg ttcaggaagc ccttcgcttt gccgtcgacg atccggagaa gcgccgagac
    21121 gccgcccgac cagttgccgt cggtgcgctc gtccgagccc gcctcggggc cgggccctcc
    21181 gacctcgttg acgacggccg agagcgactc ggacgtccgg tcgtaccgtg cgccgaacgt
    21241 cagcgcgagc cacttccacg gcgtccacgt gtcgttcgca tagaagcccc agaacgtccg
    21301 cttgtcctcc gcggagcggg tgtcgcccac cgggagcgag tcgacggacg gtacgacggg
    21361 gttcgagccg gtcgtgagcg tgaggtcgaa gcccgtcccc tcgacggtgg tcttgccgga
    21421 cgtgagcgcg atgcccgtga cgaggcggtg cgagccggcc gtcgtcacga cgcgcgcgtc
    21481 ctcgaagaag gtcgtctgga gggggcgcag cgacgttcca ttggcggtcg ccgtgttccc
    21541 gtcgacgccg tcgatgaacg accgcacggc gatctgttcg tcgcgcgcga ggccgagcgt
    21601 gttctccacc tgcacctggg cggagagcct tcgaacgagc gtcgacgtca gggagtagac
    21661 gcggtggtcc atgcgggcgc cgccgaccgc gtagttccgg tcgacctcga agccgggcac
    21721 gaactcgccg cccgcgatgg ggagcggcga gccccagtag gccgtgtccc gaaggacggc
    21781 gagcgtgagg tcccacgagc cctcgcgcgt ctttcccccg aaccctatcg agagccggtc
    21841 cacggacgat ccggtgttgt cctggaagcc gttgtcacgg aacatcgagc cggcgacgtg
    21901 cgtgatggag ttcccgtcgt gcttctccca ggcgagggac gcgtgcttgt cgttccagga
    21961 gccgagaccg agggtcacgc tcgtgttctt cccgcgccgc gtgaagacct gcaccatgcc
    22021 cgcgaacgcg gagacgccgt agagcgtgcc ctggggcccc ttggcgatct cgatgcgctc
    22081 gatgtcctcg atcgggatct gcgcgaggtt cgggttgaag ggcccgcccg cggggacgcc
    22141 gtctacggtc acgagaagcg cgtcgaactc cttcaatccc cacatcccga ggttggaaaa
    22201 gcgcgggccg ccgtccgagc cgccgccggc ctcgacgccc acgacgttct ggagggcgtc
    22261 ctgcagcgtg cgcgcgccgg tccggcgcag ctcctcgccc gtcacgacct cgacggagcc
    22321 cggcacgtcc agcgtgtcct cgggggtctt ggatcccgag acgaccatct gggcggacgc
    22381 tgacggggtc ggcacccggg gagcctgggc cccggcggcg ggggcaaaag acgcggcgag
    22441 ggcgagagcg aaggcgaaga gaggtttcac cgggtgatgt taactaaact ccagcccatg
    22501 aggatcggga tcgacttcgg gacgacgcac accgtcgtcg ccctcgtgga tcgcgggaac
    22561 tacccggtgg tcgcgttcga ggggggcgac ttcgtgccgt cgctcgtcgc cgcgcacgaa
    22621 tcgtccggta cgctccgctg gggatgggac gcggccgcgg tgaggcacga gcccggctgg
    22681 acgatccttc cctccgtcaa gcggctcctc tccgacgcgg gcccgtccac ggaagtcgcg
    22741 gtggggcgga ggcgcctccc gatcgccgcc ctcctcgagg gcttcttctc gtcgctccac
    22801 cgggagctcg tcgcgcgctc gaacgcgggc atcgccgcga acgactcaat cacggcggcg
    22861 gtgtcggtcc cggcgaacgc gtcgacggcg cagcgattcc tgacgctcga ggcgttcaag
    22921 agcggcggtt tcgccgtgga ggcgctcctg aacgagccgt cggcggcggg cttcgagtac
    22981 gcgcatcgca accggcgcga cggacggcgc gagcacctcg tcgtgtacga cctcggcggc
    23041 gggacgttcg acgcgtcgct cttgcgcatg accgggaaga cgaacgaggt cgtggcgacg
    23101 cacggcgttc agcgcctggg cggggacgat ttcgacgcgg cgatcctcgc gctcgtcctc
    23161 gagaaggctc acgcgcacga cgtctccgac gccgagcggg cgctcgtcct cgaggaatgc
    23221 gtgagagcca aggagtccct ctccccgaac tcgcgccggt tcctcgtgga cctctccgcg
    23281 ctggagaaag agcctctcgt ccttcccgtc gacgaggtct gggaagtctg cgtgccgctc
    23341 gtcgcgcgca ccctcgaggc cgtcggcgcc ctcgtggagg atgggggcgg cgtcgcgtgg
    23401 gcggacgtcg cgtcgctcta cgtcgtcggc ggagcgggcc tcctgccgct cgtgccgcgc
    23461 ctcctgaagg agcgcttcgg cgagaagcgc gtcaagcgct cgccccaccc gttcgcggcg
    23521 acggccatgg gcctcgcggt ctggctggac cgcgaggaag ggtacgcgct cgaggaccgg
    23581 ctctcgcggc acttcggcgt cttccgcgag gccggggggg gcgaggacgt cgcgttcgac
    23641 acgatcttcg agaaggggac gcgtctgccc gcgcccggcg aggcgccggt cgtcgtgacc
    23701 cgccggtacc gcgcggcgca cgacgtgggc cacttccggt tcctcgagtg ctcgcgcctc
    23761 acggacgggc ggccggacgg cgacgtgacg ccgtgggacg aggcgtactt cccgttcgat
    23821 ccggcgctgc gttcgtcgga tctcgccgcg gctccggtgc ggcggcgcga ggacggtccc
    23881 gaggtcgccg agacctatgc actcgggagc gagggcgccg tcaccgtgac cgtcacggat
    23941 gcgaaggacg gttttgcgcg gacgtttcgc ctgacgcgct gaccgtctct tcgatcctca
    24001 ggacgaggag cgtgcggtcg tcgtcggtct cgtcgtgccc ggtgtgcgcg cgctgcgacg
    24061 cgaggatgcg gtcgcggagg gcggcggccg agccgcccgc gccgtccttc aggcactgct
    24121 cgaggcggga gaagccccac ggctccccgc cctcttccgc cgctgcctcg acgaggccgt
    24181 ccgagtagac gacccagagg tccccgggca ggagcggcgc cgtcacggtc tgccagccgg
    24241 gcggcagcgc gacgccgagc gggcgggccg cgttcacgag cgcctccacg gtcccgtcgg
    24301 gccgcacgcg gtacgggtac gggtggccgg cgttcgtgaa cgacaccgtc gcggtgccga
    24361 agtcgaaggc gaggtgggcg agcgtcacga acgtccgcgg gtccgtcgag cggacgacct
    24421 cgacgtcgag gagctccatg agcctcgccc cggaggcgcc ggtcttcgcg agcgccgaga
    24481 gcgccgcttt cgaggcggcc atcacgatgc ccgtcggaag gccgtggccg gagacgtccg
    24541 ccacgacgac cgtcagcgtc ctcgcgtcgc ccgacaggtc gtagaagtcg ccgccgatgg
    24601 cggccgcggg ccggaagtcg acggcgatcg agacgccggg gaaagagaag cccgccggcg
    24661 ggaggaggcg ccgctgcagg tcgcgcgcga cctcgagctc gcgctcgagc gcttccttct
    24721 cggcccgcgc cgcgacgctc gccgcgaggc gttcggccat gtgattgaag ctttcgacga
    24781 gcgccgcgag ctggtcgtgc cccttgagca gggcgcggtg gccgaagttg cccttctcga
    24841 tctcgccgaa tcccgtggac agcctccgcg tcgcgcgcgc gatccggtag acgaggaggc
    24901 cggccacgaa ggacgcgaga aagaagacga cgagcgtcgc gatccctagg ccgcccacga
    24961 ccgcgaggac gacgcggctc atcgggacgt gttccgaccc cgagaccgtc gtgtcacgga
    25021 agagctcctg cgcctcgcgc tccagggagg tgcggacgag gaagaggacc ggctgcttct
    25081 cgaggggctg gccggtttcc gcgtcgtgga cgggctcgcg caggaacagg gggaacgcgc
    25141 tccagcggcc gctccacggt cccgacccgg ccgggggcgc ggccgtgtcg ccgccggtct
    25201 cgccctcgcc tcccgaatcg gcctcctgcg agaggctgac gctcttcttc ttccttccgc
    25261 cctcgtcgaa ggagaaccgc accgttccct tcttctcgtc gacgacctct tcgcggacct
    25321 tcacggcccc gaggaggcgg atcacgttgc cggtctcccg ctcgaggtcg cggcggagcc
    25381 cggaatcgag ccgctccgtc aggaggaacg gcccgtccgg ccggggcacg acggcgacct
    25441 cgtagatctc ggacccctgc ttgacgaggt acacgccgcg ctctttcatc caggggcgcg
    25501 gagcgacgcc ggcgggcgcg gacggtccgc ggagcgcgag atcccgcgcg cggctgagga
    25561 ggcccgcgtt ccttttcgcg agcgcggcct cgacgcggcg cgtcccgagc tggcccgcga
    25621 cgacgaggag gccggcgtag acgaagaacg ccgcaaagac gacggcggac atgaggagca
    25681 ggtacgagac gacgagccgg ctcccgacgc gccacaggag gccgcgcaga agtgcgcgcg
    25741 tcgcgctcac gcagaagacg gcgccgccga agacgaggag gacgatgccg aggcccgccc
    25801 agggcgtctc gttgcgggcc agcgtgacgg cgagaagccc ggcagcggcg agaaggccgc
    25861 tgaccttgaa cgtccgcgag agcttcatcg tggtgcgctc ggggccgcct gcggcgcgcc
    25921 cggctcgagc gcccgggcct ccgacgcgag ataggtcgtc tgcgtcggga acgcgaagcc
    25981 gctgcccgct ccctcgacgg cgtgcatgag cgcgaggagc agctcctcct gcacggcgag
    26041 gtatgccgcc cagtccggcg cgaggacgta ggcgaagacc tcgacgtcga gcgtgtaggc
    26101 gttcagcttc aggaagcgca cgcgcgccgt cgcgggctcg acgcgggggt cggcggcgag
    26161 gcgcgcgcgg caggcctcga ggacggcctg catctgggcc gcggtggtct cgaagttgag
    26221 gccgagcgtg tgccggaaca ggaacttgtc gcggcgggag aggttctcga tctgcgaggt
    26281 catcatcatc ccgttcggga tcgagacgac ggtcctctcg aacgtgcgga tcctcgtcgc
    26341 gtagagcgtc acgtcctcga cttcgccctg gacggtgccg accttcacga cgtcgccgac
    26401 gcgcaggatc ttgtccgtga ggacgacgac gcctccgaac aggttctcga ggctcttctg
    26461 ggccgcgaag gcgaccgcga tgccgccgat cccgaggccc gcgagcgtcg cggtgaggtt
    26521 cacgccgagg acgccgagcg ccgcgatggc ggtgccgagg acgacgattc cctgcaggac
    26581 gcggcggccg atcgtgaggg aggacgtcgc ggcctgcccg gacgcgagga gccgcatcgt
    26641 cgcccgcgac gtcaggaagc cgacgatctg gacgaagagc cagccgacgg tgaagacgag
    26701 cgcgaggcgc acgccgcggg cataggcgta ccggccgagg agcgggagtc cgatccggtc
    26761 gacgaagaag gcgtggaggg ggatcgcgag aaggagagcg agcggcgcgc ggaaggacgc
    26821 gaggccgtgc cggaagtccg cgaaggcctg gcggcgactc gccgtccggt cgatgaggcg
    26881 agccgcgccg tacacgagga gccgcgcgag gagccaggcg gcggggatca gcaggaggaa
    26941 cgcgaggatc tgccagagtc ggaccgagcc tccgacctgg cggcggagcg gcgccggcat
    27001 gatccggtcc agctcgggga ccgagaggtc ctggaaggcc actgggatct cgcggagcgt
    27061 ctgcgacgag acgagccaga tcgcggggcc ctcggccggc cgcgtccgga cgaggagcac
    27121 gtcgaaggtc tcgtccccgc gatggaccgt accggcacgc tcgtagtcgg gcccgagccc
    27181 gtcgtccacg ctgctgagcg gggagcggct gagcttctcg aggtcgcccg tgaagcgctc
    27241 gtcgacgacg gctttcagct cccgcgcgat ctcctcgcgc gacgtcttcg cgcctttcgg
    27301 ccactggaga tactcggccg ccgccgtcca gttctcccgg gcggaagccc gcatgaatcc
    27361 gagaaacgcg ccgtacggcg tctcgcgctt gtaggggtcg ccgggcgcct cggccgcggc
    27421 cggcgtcggc gtcggggaga gcccgggaat ctgcgccgcg agcgggccgg cggcgaagag
    27481 cgcggcgccc agggcgaaag cggcgagagt tggtcggttc ggaactgcca tgcggagggc
    27541 cagcctaccg caccgcggcg cgcatccatg gcggccgcgc cctcgcgatc tttctcgcca
    27601 cggggcaggc cggcgcgtgc gcgccgggga gccagccggt cgacatgagg aactcgttca
    27661 cgatctctcc gccggtgaaa cggaaggtgg agcggaagag tttcacccat tcgtccttcg
    27721 gaaggggatg gtgcgcggcg agccagccgt cgaacgaccc gtgcgtctcg cgcagggcga
    27781 ggacgacgcc cgcgttgtgg atcacggcgt cgaccttgag acggttgcgg acgattcccg
    27841 cgtccgcgag gaggcgccgc cgttgccggg cgccgtagcg cgcgacgacc tcgggatcga
    27901 agccctcgaa cgcgcggtgg aaggcctcgc gcttcctgag gatcgtcgcc cacgagaggc
    27961 cggcctgggc gatctcgagc gcgaggcgtt cgaggaggcc cgcgtcgtcc gtgagcggga
    28021 agccgtactc ggtgtcgtgg tacggaccgt ggagcggatc gccgggagcg acgcggcagt
    28081 agccggacat cagaacgggt ccgggccgtc catcgcgctc gcgggcggct ccggccgccg
    28141 cggcgggcgg acgaccgtga cggagcagtc ggccttagag gcgacctcgg cggagacgct
    28201 cccgagaatc gagcgcctca ccgaatcggt ccgcgcgccg atcacgacgt ggtcgacgtg
    28261 gttcgcctcg acgtactcga ggatcgcctc ggcggggtcc ttcgcctcga ggacgtgaaa
    28321 cgtgaggcgc gtctcgccga tcgcgagggg ccgggcccag tgtttgagct cgacgaggcg
    28381 ctccacgtgc ttgttgcggc ccgcttcgtc cagcgtcgtg tcgatcccga tgcggcgctg
    28441 cttgaggacg ttcaggatcg cgaagcgcgc gcccgggacg atctcgatga ggcgccggac
    28501 cgtcacgcgc agcgcttcga ggagctcctc gtcgccgtcg aggtcgacgg ccgccatcac
    28561 gatgggcgtg tcggccttcg gaggcgcgat ctcgcggagc gccgtgggac gcacctccgg
    28621 gttgaagcgg cggcggaccc gggcgctcca cgggtcgcgc tccatcttct cggagcgcgc
    28681 ggtgagcttg acctggtcgg gatgggcgag gtcgaacgcg acgtacgcgg ccgtcgggta
    28741 gcggcgcagc gggtcgacct cgaggcagcg caggacgacc tcctggagcc acggcggata
    28801 gtcgtccttg aggcgccgcg gcggcacggg gtcccaccag atccgcctct tcagggcgcg
    28861 cgcgcccttc gggtctccga acgggcggac gcccgtcgag aagaagtaga gcaggacgcc
    28921 cagcgcgaag atgtcgctcc tcgggtcggt gcggttgccg aggatctgct cgggcgccat
    28981 gtagggcgcc gtgccgtacg gcacgcggaa ctcctcgccc atgaggtccg gcagctggtc
    29041 gtgatgggcg aggccgaagt ccacgagcgc ggcctcgccg ctctcgcgca tgatgatgtt
    29101 cgagggcttg atgtcgaggt ggacgacgtt ctggtggtgg aggtcgagca gggcggcggc
    29161 gaccttgttc ccgatccgcg cgacctcggg gtagggcagc ggaaggtcgt cgagccgcgt
    29221 gaggagcgtg cgcccctcga tgtgctccat gacgatgtag ggctgcacgg cgaagtcgcc
    29281 cgacgcgacg aacttcggca cgtggatgcc ggacaggcgc ggaaggatca tctgctccat
    29341 ctcgaagccc acgatcgcag ccgggtcctc gccttcgagc atcgcgggga ccttcatgag
    29401 gagcggcatc gagatcccgg ggcgcgagac cttccggagc gtcgccatcc cgcccgtgtg
    29461 gaggaggtcc ccgacgacga agccgtcgat cacggcgccc ttctggacgc ggacgcggac
    29521 gttcatgcgc tcactggccc cacgtcagtc gctcggcgta catctcgggg agcgcggcgc
    29581 gccggatctt cttcgcggcc tcgtcgacct cgtacgggac gcggtggtac gtgatctcgg
    29641 cgcgcgccgt gtccaccagc gcccagcacg cggccgggtt gccgtcccgc ggctgcccga
    29701 cggagcccat cacggcgagc cagcggcgct ggggaaggag cgggatcgcg acgcccggca
    29761 cgggccggaa cgtgatgagc ttgagcgtct cggacatgtt gtagacggcc ggccggtgca
    29821 cgtggccgca gaacgtgacg cgccggtgcg tcgccttgag acttcgcgcc gcgtccgccg
    29881 cgtccgtcac gtacgtccag cgccccgggg cggaagcgtc cgcgtggacg aacaggcagt
    29941 caccctcctc gaccgtgagc ggaagcgcgg cgaggaacgc gcgctggtcg gctccgagct
    30001 ggttgcgcgt ccactcgagg gcggactcgg cggaggagtc catcgtcggg ttcctcccgg
    30061 ccaccgcggc gtcgtggttg ccccggagcg cgacggcgag gcccttcgcg acgcggtcca
    30121 tgatcgcccc gacgacccag tcgggatccg ccccgtagtt cacatagtcg ccgaggagga
    30181 cgagccgctc ggcgcggcgc gcggcggcgt cggcgagaca ggcctcgaac gcttcccggt
    30241 tggcgtggat gtccgccatg agcgcgagcc gcatggcagg agcgtatcgc gaccgggggc
    30301 gcgggggaga ggggtggtac cgcgtacggg atttgaaccc gtgatctccg ccttgagagg
    30361 gcggcgtcct gaaccgctag acgaacgcgg cacgttttcc caccacaaag aggcgtcccc
    30421 acgtgcgcgg ggacgcggaa tctagcacgg ccggcggcgc cgccacaaat cgcggaggga
    30481 ggaggagaat ccccccatgg aattcggaat cctcggcctc gagctctccg gcaagacgac
    30541 catcttctcg ctcctcacgg gccacgcgac ggcgggaacc ccccacaagc gcgaggccca
    30601 cgtcgggatc gcccacgtcc cggacgcgcg gctggacaag ctcacggcga tcttcaagcc
    30661 gaagaagcac acccccgcga ccgtgaagtt cgtggacgtc cccggggtcg cgaagggcgg
    30721 cgcccagagc ctgaacctcc ccgagctgcg ccagatggac ggcctcgcgg tcgtcgtgcg
    30781 cgggttcgag tccgacgcgg tcccccaccc ggagggctcc gtcgacccgg cgcgcgacct
    30841 cgagctcctc gagacggaga tgctcctcgc ggacctctcc gtcgtcacga accgcctcga
    30901 gcgcgtcgag aaggacatcg cgaagaagaa gacgcccgag ctccagacgg agaaggacct
    30961 cctcgtgcgc ttcaaggccg ccctcgaccg gggcgagccc ctgcgcggct tcgacgtgca
    31021 cgtcgaggag gagaagtacg tcaagggctt cggcctcctg acgcgcaagc ccatgctcgt
    31081 gatcctgaac gcggacgaga gcgacgcggc cgacctcgcg ggcgcgctcg cgaaggcgaa
    31141 gctcgacgcg tggaagggca agccgcacgt cgcgttcagc gccgtctcgg cgccgatcga
    31201 gcaggagatc gccgcgatgt cgccggagga ccaggccgag ttcctcaagg gcctcggcct
    31261 cccggaccgc gcgctcgacc ggctgctgcg cgccgcgtac gacctcctcg gcctcatgtc
    31321 gttcctcacg gcgggcgagg acgagtgccg ggcgtggtcg atcccgaagg ggacgcacgc
    31381 cgcgaaggcc gcggctgcga tccacaccga cttcgagaag cacttcatcc gcgccgaggt
    31441 cgtgccgttc gagacgctcg tcgaggccgg aggctcgctc gcccactgca agacgcacgg
    31501 cacgctgcgc gtcgaaggcc gcgactacgt cgtccaggac ggcgacgtca tcaacttccg
    31561 ggtttcggcg tagatccggg agggcgcagg cggcgcagcc gccgagcgga gggtgtggga
    31621 acccgcgtga cgggttccca cttgagagtc agtccgcccg cagcgcccag cggaagatct
    31681 cgggcagcgt cggcttcttg ccgtacatga ggatcccgac gcggtagacg cgccccgcga
    31741 accaggcgac gaggacgatc gtcgcgacga ggagcgtgac ggacgtcgcg atctgccaga
    31801 gcggcggcgt ctggacggag atccgcatga acatcatgag cggcgccgtg aacgggaaga
    31861 acgagaggat cgtcgcgagg cggccgttcg gctggttgaa cgcgaagaac cacgtcgtgg
    31921 acgtgaggat gagcatcatg ccggggatca tcacgaactg ctgcgcctcc tgctccgtgt
    31981 tgaacggcgc cgagagcgcg gcgtagagcg acgcgtagag gaaatagccg agcacgaaga
    32041 agacgacgaa cgcgccgatc gtcgcgaccg ggatgtgggg gaggccctcg cccatgcccg
    32101 cgagcgcggc gacgccgggc agcgtgatcg cgagcgcgag cgcggcccag accgcgtact
    32161 gcgtgaggcc cacgaggccg atcccgagga tcttcccgag catgagcgtc gtcggccgga
    32221 tggacgagac gatgacctcg acgatgcggt tgttcttctc ctcgaggacg ccgcgcatga
    32281 tgtacgcgcc gtacacgaag atcgagatgt agatgaggaa gaagaacgtc gccgagatcg
    32341 cgaggttcat tcccacgttc ttgccgctct cgccgctctc gacttccttg gcctcgtgcg
    32401 gctcgaggtc gacgcgcagg cgcgcggcct cgtagacctt cgggtctacg cccttgtcct
    32461 tgaggcgttc cttcgtcgcg gcgcgcgaga ggaggccggc gatctgttcg cgcatgacga
    32521 cctcggcctt cacgctctgg gcccgccact cggcatggcc ggtttcgagc gtccggggcg
    32581 tcaggacgat gtaggccttg atcttctcgg actggacgtc cttgttgagc ctcttgcgga
    32641 cgtccggaag cgtctcgggc gtcgcgtcca ccttgaagag gtcgagctgc gtcaggatct
    32701 tctggctgcg gctctcgttc atcttctcgg tgacggactt ctcgtcgacc ttcgtcgtcg
    32761 gcgccttctc gtccttcttc ggcttctcgg cctcctcgtc ggacggacgg gccttgtact
    32821 cggcgacgag cggctcgtag agccggcccg agaggtccac gacggcgatg cgccccttcg
    32881 cgacgagcgt cttggtcagg gcgacctgga tgaagatcat gccgaggccg agcgtcggga
    32941 tcaggaacgt cgcgacccag aacgccttcc cgcgcacctg ctggaggtat tcgcgcttga
    33001 tgacggcgag cagcttggaa tccatggcta cttcagctcc tcggcggtcg gcgccacgag
    33061 gccggactcc tggacgcact tgatgaagat gttctcgagc tcgggctcgg gctcgacggg
    33121 cggcggcagc ggcacgcggt cccggtagga gtgcttgatg tccttgaccg tgccgtccag
    33181 gagtttctgc gcgcggtaga ggagtgcgat gcgctcgcag aggcgctcgg cctgctcgag
    33241 gacgtgcgtc gagaagacca ccgtcttgcc ctgcttcttg aaggcggtca ggaagtcctt
    33301 cacgaggacg acgttgatcg ggtcgaggcc gctgaacggc tcgtcgagga tcaggatctc
    33361 ggggtcgtgg acgatcgtgc cgaggaactg gaccttctgc tgcatgccct tgctgagctc
    33421 ctcggtcttc ttctggagcc aggggcggag gtccatcgag tcgatccacg gcgagaggcg
    33481 cttcatcgcg gtccccttgt cgacgctctt gatcgccgcg aagaacagga gctggtcgat
    33541 gaccttcatc ttccggtaga ggccgcgctc ctcggggaga tagccgatcc gctcctggag
    33601 cttctcgttc atgacctcgc cgaggacccg gatctctccc tcgtccggct tcgtgatgtt
    33661 catgatcatg cggatcgtcg tcgtcttccc ggcgccgttc gggccgagga gcccgaacgt
    33721 gacgccccgg ggaatctcga ggtcgagctt gttcacggcc gtgaacttgc cgaagcgctt
    33781 cacgacaccg tggagagaga tcgcggcagt catggattca cttcctcctg cgggagccgg
    33841 gaagatatac gggcgggcgg ggacgaagtt taggtcggcg ggggccaccg ccgctaaacg
    33901 ccctttccgg cagggacttg cgccgcgccc cggactccgc tatggtccgc tgcccgcggt
    33961 cctcgcgtgc gcccatagct caactggatc agagcgtttg actacggatc aaaaggttgg
    34021 gggttcgagt cctcctgggc gcaccactta gatccgggcc cgtcctgcct cctataattc
    34081 cttcacacgc aagaaggaga cggtcatgag gcgaatcgct ttcgtcgcgg cgacgctggt
    34141 cctgttcgcg gtttctacgg ctccttccct tgcgcaggaa gcgcgaccgc ccgagggggc
    34201 acgggacgaa accgcgcgcg agatggccgt tctgaaacgc gaggtggacc ggctgaagac
    34261 agaggtgact gcggcgagga ggcagatcga ggagctgaag aaggcccagg ccgacgtcaa
    34321 cgtccacgtc gagatgcttc tcaagcaccg ccatcagctc ttcctcgact tcatgcgcgc
    34381 ggacgccttc ttcccgcagg cgaaggggac cttcttcgtc acgaccccgt cgcccgacac
    34441 cctgacgaag acgagcggcc cggtcctggg cccgaaatga cccgagatcg gcgtgaggtg
    34501 accccgtgaa cggatcgctg cgttcgaccc tcctgctcgg ttccgtcctg cttctcgcgc
    34561 ccggctgctc gtccgggccg gtcgaggtca aggccgctcc ggacgcccgc gccgacgcgg
    34621 cgtgcccttc gggcgccggc gcgcggttga ccgggacctc gttcgcccgg cagaggaacg
    34681 ggaagctggt cccgggcggg ggccggccgg tctacctcga cccggcgacc cgctattccg
    34741 ccgcggtctt ccgggcgatc gtcgagcacc agaacaagaa gagctatttc tcggccgaga
    34801 aggagagcgg gacggtcgtg cccgaccccg ccatgttcaa gtgccgccgg accgtccagg
    34861 cggacgccga gggcaagttc acgtttgcgg acgtgccgcc cgggtcgtac ttcgtctcgt
    34921 cctacgtgtc gtggatcaag cccgacggaa cgggtgagtg gtccggcgtc tggaatctca
    34981 gccccgtcgt catctccgcc gacgggaagg acgtgaacgt ggtcctgagc ggcgggccgg
    35041 agacgatcgc agacccgccc gcgcgctgat cccgtaagct cgtgccttga atcacgcgga
    35101 cgacgatctg ttcatgcggc tggcgctcat ggcggcgcgc cgcgcgcccg gccacgcgga
    35161 cgtgcccgtc ggcgcggtcg tcgtgaagga cggagccgtg atcgcccgcg cgtggaacgc
    35221 gcgtgaaaaa ggagccgacc cgaccgcgca cgccgagatc ctcgcgatcc ggaaggcggc
    35281 gaaaaagctc ggctcgtggc ggctttccgg ttgcacgctc tacgtcacgc tcgagccgtg
    35341 cgcgatgtgc gccggggcga tcgtcctctc gcggcttccg cacctcgtgt acggcgcctc
    35401 cgacccgaag gcggggttcg tcggctcgct cggagacctc tgccgcgacc cgcgcctcaa
    35461 ccaccggccg gccgtgacgg cgggggtcct cgcagacgaa tgcgggagaa tcctcgtgga
    35521 gttcttccgt gaaaaacgcc ggaccgcccg cgccgcccgc tgagaacgga ttcggagccc
    35581 ccgaggcgcc cgcgctgcgt tgcccgtcct gcggagccgc ggcccgcgaa ggagaccgcg
    35641 cgtgcgcgca ctgcgggtcg ctcctcgcga cccggcgctg cgtcgcgtgc ttcgcgctct
    35701 ccccgcgcga cgccgagaag tgcgtgcgct gcggcgccct cctgcccgcc gaggcgctga
    35761 acgccgttcc ggggacctgt ccggactgca aggcgggctt cgtcgcgcgg caggcgggag
    35821 tggcgggctt ctccgagtgc ccgcgctgcg gcgggctctt cctggcgcgg gcgtcgttcg
    35881 acgcggtcgt gaaggacgcc gcgacgcgcg aacaggcgcg cgccctcgac gcgggccccg
    35941 agcgtgccgc cccggaacac ggcttccact accggccgtg ccccgtctgc cggaagttca
    36001 tgaaccgctc gaacttcggc ggcggctcgg gggtgatcgt ggacgtctgc gggcaccacg
    36061 gcgccttcct cgaccgtggc gagctgaccc ggatcgtgga cttcgtcgag aagggcggct
    36121 gggagagggt gaagaagagg gagatccttc gactggagga agaggccgcc gcgctcgaga
    36181 gccgcaagcg tgtggccgcg ggcctcgggc aggccctcga cgccccgccg cgcggcggcg
    36241 ggctggcgga ggtgctctcg ttcctcgggt cgatccttcg gtcgtagtcc tccgnnnnnn
    36301 nnnnnnnnnn nngcgcgggc cgacgccggc gggggggccc gagtcttgcg atgtgacgtg
    36361 acaaagggga gcccatgaag caggtcgtcg ccgctctctc gatcctcgcc ctctgcctct
    36421 tgacgcccgc gctttcggcg gacacgccgg accagccgcc ttcgcgtgat ctggattcct
    36481 cggatcgcgc gggagaccgg tacgcgctct cgatgattcg ggcgggacac gcgaccggcc
    36541 gtccctggcc cgagatcgca cgcctctcct gggacaaccc ggcgcgcttc gcggagatcg
    36601 tccttccgcc ggaagggccg cgggtcctcc gcgaggagat gcctgccgac tacgttcgcg
    36661 cgatcgaagc ggaagcggcg cgcgacgaag cctacgcgct tggcgaggcc cggctctggc
    36721 gcgagggttg gctgcgcatc gcgagagacg tcgtgccgca gctcgacagc aaggacgcgc
    36781 cgggcgcgag gcggatcgca ctggagttcc tgaagctcga gaacgcgggc gacgggctcg
    36841 ggttcgactg gcgggaggag cccgacacac cgggcaaccg cgcggcgcgc gcacgctgga
    36901 aggcgttgct cgaagcgccc cggttcgccg ccagcggcac cttcaggcac gtcgtcgcgg
    36961 acgtcgtgag gatgcgggcc gcgccttctc cgtccgcccc cgtcacggcg cgcctccgga
    37021 tcaacacgtg ggtacgcgtc gcccggaccg acggcgagta cgtcgaggtc gtcgtccggg
    37081 cgggcgggag ccccgcgaga ggattcgtcg agcggcggtt cgtcgcggaa cagtcgaggc
    37141 tggacgtgga tcccgtgctc gagaccgctc gaaaggaggc cgcggcggga cgtcccctcg
    37201 aggccctgac gtgggccgag cgcgcgtttg ccctcgaccg ctcgagcgag gctgcgctgg
    37261 acctgctcga gaagacggcg cgcgacgcgg gtcaacccga aagggccgag cccgcgcggc
    37321 gcctccggac gggacaggtc ccgatcacgt tcggcgtgtg tgcccgggcg gggatggaca
    37381 cggacttctt ccagctcgtc gtcgtcgcga atctgtcccc gaagggcgag ctctcgccgg
    37441 tgtccccggg cgatggcgcg cgcctcgcct cggaggcctg gttcgtcgtc cggccctccg
    37501 ttccgggcgc tcgcggcgag ccgttctacg ggacgcccac cgtccggccg cgccggcttt
    37561 cctacatgta cggcgaggga accctcctct cgctgggaga ggcatgcaag ccgggcgagg
    37621 tctgggcgac cgttcccttg agggaggtgc gccggtcggg actcaacgcg gcggccctcg
    37681 aggggaagct cgagaccttc ctcaaggaag gattcagcga tgacgtccgg gggagcgtcg
    37741 ttctcgacgc ggtcgaggcg cccaccctgc tcgacggagc cccggccgaa agcgtcctcg
    37801 tccggggccg ggtgcgccgg gcacgcacgg acagcgagcc gggaacctat ccggtttccc
    37861 agtgggcgct cttcggcgcg ggcggccgga tgctgcagcg cgtgggcgac gtgtccttcg
    37921 gcgcgacgga cgtcggcgag gttctctgga cggcgcccgc gttcggcccc gcgtggcgcc
    37981 tcggattcgt cctcacggac tccagcgccg gaacgacggc gaacggcgac gttctcgtcg
    38041 tcctcgtcga cccggaaggc aacgtgaaga cgcttcccgt gcacctctgg gacggcggct
    38101 gctgaacgcg gtccgcttga cgccccggcc cgttcgggta acctccgcgg tctgccgcgc
    38161 tgcggacagg tgtccgagtg gtttaaggag cacggttgga agccgtgtgt agctgaaa
//
LOCUS       DAOMFY010000009        37678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_347_length_37678_cov_7.190974, whole genome shotgun sequence.
ACCESSION   DAOMFY010000009 DAOMFY010000000
VERSION     DAOMFY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 37678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_347_length_37678_cov_7.190974"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="PLB01_01745"
     CDS             complement(<1..1708)
                     /locus_tag="PLB01_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQQ76049.1"
                     /translation="MSDREPTRAPASPPAAAWHARHVDDVAAALGTHVELGLPPSRLA
                     ERPPDDAVHTEGRGPWRIFGNQLKGGVILVLIAATAVMLALGHVGDALAIGASVVFSV
                     VFGFVTDFRAERALEALRNLSAPTARVVRGGLEREIPAEDLRPGDLLVLMGGQIVAAD
                     GRVAVANDLQADESALTGESVPVAKSPEPVEAAAPLPERTSMAWAGTTVVSGSGRILV
                     TGIGGGTELGRIGKLVADQGRQETPLEKQAEQLGRRLALLAIGLSALVTVLGFLRARP
                     FWLMLETGLILAIAAIPEGLPAVTTIALAAGVRRMAKADSLVRRLSSVETLGCASVIC
                     TDKTGTLTENVMRATRVVLPGRSLDVTGERYDPAGEFLEAGRPAPPGDDLTRLLEIAA
                     VCNDATLESHEGWHVHGTSTEGALLALAAKGGVDGSRFERLSAVAFSSERKRMSVLAR
                     DGEGRIWSFVKGSPESLATRAARILSGGAEVPLDAAGRERLLASAHALGASGHRVLAF
                     ACRALGADDDAEAGEEGLTWVGLVGFIDPPRANVRGAIEELRLAGIRTVMVTGDQKGP
                     AMA"
     gene            1862..2143
                     /locus_tag="PLB01_01750"
     CDS             1862..2143
                     /locus_tag="PLB01_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQQ76050.1"
                     /translation="MPASAAAAVSTLADLVPGQSGVLTLLDLPEGDAQRLMELGFLPG
                     SVIVAARSAPGGDPRVYRVEGTEFALRRDTARHIHVEGSSIGRAAGEGR"
     gene            2140..4041
                     /locus_tag="PLB01_01755"
     CDS             2140..4041
                     /locus_tag="PLB01_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HQQ76051.1"
                     /translation="MSAASQCHGCPVAAAPIAVHGHPHPRLVGLVGPPNSGKSTLFNR
                     LTGLRQKVANYPGVTVEERRGRARLDGDTEVVLIDLPGVYSLTPRSEDERVTRDVLAG
                     EMAGQQKPDAILLILDSTNLKRHLVLAAPVLALGLPTLVVLNMADELEARGGSVDPAA
                     LAGQLGAPVALVSAAKGEGIETVYDFLNGAVGVPRAMDLPTLADIPKCRQWAVRVGDQ
                     AGYAAPAPPVWTRRLDAVFLHPVAGPLIFLAVVVAVFQTIFSAARPLMDLVDAAVRAS
                     GAWVGALLPASAMRSLLVEGVWGGVGSVLVFLPQILLLFLFIGLLEDSGYLARAALIA
                     DRTMRKAGLQGKSFLPLLSAYACAVPAVLASRTIENKRDRIATILIAPFMTCSARLPV
                     YTLIIAAFIPERALLGPFLGTRAAAMLGLYVLGFLAAFGTARLLKSSVLKSDGAPFLL
                     EMPPYRRPTLRQIALRMFDRSKIFLRRAGTVILGVAIVLWVLAHLPLSAGQMPEIGNS
                     VAGTLGRALEPLVRPLGFDWRIAIGLVTSLAAREVIVGTLGTLYGMDAHSGTAGLQAA
                     LRHDLTPGGAVALLVFFAFAMQCLSTVAVVRRETGGWRWPAIQFAYMSLLAYSGAFVA
                     NRVVTALLG"
     gene            4077..4580
                     /locus_tag="PLB01_01760"
     CDS             4077..4580
                     /locus_tag="PLB01_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQQ76052.1"
                     /translation="MNRKKFFDAAAHWDVETVSAGLREDRGLARVVDERGRTALHRCA
                     RTNGEKDGRDVAASLRVAKALLKAGANPDAVHDIPNEGGSFKATPLWYAVAWGRNPRL
                     VQLLLDDGASPRGCLWACVWANDVEMAKTLVKAGAPLNEASGGETPLAYATRLRRPAF
                     YRILGGR"
     gene            complement(4594..4809)
                     /locus_tag="PLB01_01765"
     CDS             complement(4594..4809)
                     /locus_tag="PLB01_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76053.1"
                     /translation="MSPAQKPSPTLPESGSSLNPEGHHTCTRCPVCATEACAVLEELG
                     MFCETMCAASQAQAAKEVEPRNSFAAA"
     gene            complement(4867..5970)
                     /locus_tag="PLB01_01770"
     CDS             complement(4867..5970)
                     /locus_tag="PLB01_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HQQ76054.1"
                     /translation="MSDPDAPREVRREDRLDEGALAEFIARAFPGDFGAAGGGARTVS
                     VSQFGAGHSNLTYLVTFGGREAVLRRPPHGASVKSAHDMRREFDVLSAVSPLYSKAPK
                     ALAFCDDPSVIGAPFFLMERVQGLVLRSSEPPPGVHLPPPRMRALSEAFVDSLAELHA
                     LDVSTGPLAALGKPEGYAARQVSGWTERWIRARTEDVPALDAAAAWLAGRVPEAARSA
                     LVHNDFKYDNLVLDPADPRRILAVLDWEMATLGDPVLDVGTTLGYWIDPDDAPEVRAL
                     PFGPTLIPGNLSREEVAARWKERTGRPDADVLFAYVFGLFKIAVIAQQIYRRFAEGHT
                     TDPRFAALLGGVRILGRQSARALDRQRIGRLGV"
     gene            complement(5967..7172)
                     /locus_tag="PLB01_01775"
     CDS             complement(5967..7172)
                     /locus_tag="PLB01_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQQ76055.1"
                     /translation="MDFSAPENVKQLRQLVRAFVEKEVVPLEPKFLNEGWPAVAPALE
                     AVRRRARGTGLFAAHLPSAWGGAGLSLVEFAHMSEELGRSPLGPYAFNVQAPDVGNME
                     LLLGHGTDEQKEHWLRPLVAGEIRSCFTMTEPEFAGSNPVWMGTTAKRDGGDWVIRGH
                     KWFASSAEGAAFAVCMAVTDPDAAPHRRASLILVPTDTAGFSILRNLSVMGHRGGGWA
                     SHAEVGYEGVRVPLANLLGSEGDGFALAQERLGPGRIHHAMRWIGVMERALEIMCRHA
                     VAREIAPGTPLGTRQLVEAAVAESRADIHATRLVVLHAAWLLEKDGPKAAREEISLVK
                     FTAARALQRVLDRAVQTLGGLGMTDDTPLAFWYAHERAARIYDGPDEVHLSFVGRHTL
                     RRFGLPHRG"
     gene            7243..8670
                     /locus_tag="PLB01_01780"
     CDS             7243..8670
                     /locus_tag="PLB01_01780"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQQ76056.1"
                     /translation="MSVLPKIIQGGMGAGVSNWTLANAVSRLGQLGVVSGTALDVILA
                     RRLQDGDPGGHMRRAIAAFPFPEMAGRVLRKYFVDGGRPEGAPYRAIPMYARTGSARE
                     LVELCIVANFVEVWLAREGHDGPVGINYLEKIQLPVLPSIYGAMLAGVAAVLMGAGIP
                     IRVPGILDHFVLHEAAEYPLAITGSIEGDDTAMRFSPADWIEGAAPALKRPLFLPIIA
                     SNTLALTLQRKANGRVDGFIIEGPTAGGHNAPPRGKPALSATGEPVYGERDVVDLAKM
                     REMGLPFWLAGGRGTAEGLQDALVGGAAGIQVGTAFAYCDESGLDAATKARVLALAAS
                     GGAHVFTDPVASPTGFPFKVVQIEGTLSSEEEYLKRTRICDLGYLREPYRREDGSTGY
                     RCASEPVAQYVAKGGGEEYAQGRKCLCNALMADIGIGQTRGGVVEKTLVTSGDDVADI
                     ARFLTSGSTSYSAADVVRRLLETAS"
     gene            complement(8675..9739)
                     /locus_tag="PLB01_01785"
     CDS             complement(8675..9739)
                     /locus_tag="PLB01_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HQQ76057.1"
                     /translation="MARLFEPLRLRGTTFRNRIFVSPMCMYSAVDGVPNDWHLVHLGS
                     RAVGGAALVIAEATSVSAEGRISPGDTGLWNGAQVDAFRRIAGFVSAQGAVPALQLAH
                     AGRKASTHVPWRGSGPLAAGEGAWTTLAPSAIPFDAGVPAPREMTSADMDRVTDEFAG
                     AARRAVDAGFGVLEVHAAHGYLLHEFLSPLSNHRKDGFGGTLENRMRFPLRIAGAVRA
                     AFPADRPVFVRISATDWVEGGWDLAQSIVFARRLKELGLDLVDCSSGGLVPNAKVAVG
                     PGYQVPFAEAIRRDASIPTGAVGMITEPKQAEEILQNGRADAIVMARAFLRDPYWPLH
                     AAKELGAKVEWPVPYGRAKV"
     gene            complement(9745..12876)
                     /locus_tag="PLB01_01790"
     CDS             complement(9745..12876)
                     /locus_tag="PLB01_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQQ76058.1"
                     /translation="MNISELFIRRPVATTLVMLAILFFGIVGYRLLPVSDLPNVDFPT
                     IQVTAALPGASPETMASAIATPLEKQFTTIAGLDNMTSTSILGVTQITLQFSLSRSLD
                     AAAQDVQAAIGAASRQLPPGMPTPPTYQKVNPADQPVLYIALTTPTLPLSVLNEYAET
                     MMAQRISTVSGVAQVLIFGSQKYAVRVQLDPRALAYRGIGIDEASAAVASANVNIPTG
                     TLWGPNTAYTVQATGQLTEAAQYGPLIIAYRNGAPVRVRDVGRAVDGVENDKTASWYV
                     DKTGPNRSIVLAVQKQPGENTVEVVSNIRRLLPQFQSALPGSAKLNILFDRSQSIRES
                     VADVKFTLWLTLALVVLVIFLFLRNLSATVIPSLALPLSIAGTFAAMYVLGYSMDNLS
                     LMALTLSVGFVVDDAIVVLENIVRHLEMGEKPFEAALNGTKEIGFTILSMTISLAAVF
                     IPLLFMGGIIGRLFREFAVTIAVAILVSGFVSLTLTPMLCARYLRAHSKEERHGRLYQ
                     ASERVFDSALAFYQRGLTWALAHQRATMAFSLVILALTGWLFVHIPKGFLPSDDTGQI
                     FAPTEAAQGISFESMIEHQKAVAAIMAEDPGVDGFMSSAGNRGNDVRGGNTGTMFIRL
                     KPRRERPPVDEIIQRLRPKLAQVPGIRVYPQVLPPIRLGGALTRAQYQFTLQASDTKD
                     LYASAPALEEKLRGVRGLQDLNSDLQLRNPTAFLTIDRDRASALGVTPESLENALYSA
                     YGSRQISTIYAPNNEYQVIMELLPEFQRDPSAFQLLYVRSKKGELVPLSAITHLERTV
                     GPLAVNHAGQLPAVTLSFNLAPGVSLGEAVTEINKVARATLPASVQTSFQGTAQAFEA
                     SQKGLGLLLLVAILVIYTVLGILYEDFLHPITILSALPFAGFGALLTLVIFRTELSIY
                     AFVGVILLVGLVKKNGIMMVDFAVEARRRGKGAEEAIYEACVVRFRPIMMTTMAALMG
                     TLPIALGFGAGAESRRPLGLAVVGGLLFSQMITLYVTPVFYVTMDRLDGWIKRRRHAS
                     SPGAVDAPAPAGG"
     gene            complement(12873..13958)
                     /locus_tag="PLB01_01795"
     CDS             complement(12873..13958)
                     /locus_tag="PLB01_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ76059.1"
                     /translation="MRRIVPAVLLAAALGGCTRAARTPPAPPSLPVTATTVEKMDVPL
                     EVTAVGHVDAYSNVAVRTRVGGEVTKVAFREGQDVKAGDLLFELDQRPYRAALAEAEA
                     NLERDRARAREADENLKRYAELVKKDYVTKEQFSQATANADALRATVKGDEAALETAR
                     LNVAYCTIKAPISGRTGGLLVHPGNLVKANDDRALVVINQVEPIYVTFSVPEAALADV
                     KKRAASGTLKVSALPNSPGAVASEGDLTFIDNTVDPTTGTINLRATFPNHERALWPGQ
                     FANVSLVLTTDAGATVVPNTAVQPGQAGSLAWVVKPDESVEPRPVTVRRVWKQWTVVE
                     KGLAPGERVVTDGQLRLSPGAKVTIKK"
     gene            complement(13955..15397)
                     /locus_tag="PLB01_01800"
     CDS             complement(13955..15397)
                     /locus_tag="PLB01_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQQ76060.1"
                     /translation="MPRSLSTSAAMLTAALCGACASAPDTPKAPEARWVAPAEAIPPA
                     PKPPPPPPEASPERLAKRKISLYEAIDVALANSPVTRQSWFLTQAAAADVGSRRSAYY
                     PVVEADLSGTRQKQSALGGSVTTLSTTYAPSVSLNWLLLDLGGRAADVEETKRRLVAA
                     NFVHDAAMNDLILGVAQAYYLYEGARALLVAQEASLKQARENYAAAEERHRAGVATIA
                     DVLQARTVVSQQKLAYDQVSGQIQTFRGQLATTLGVPVDLPVEAADLPEDVHEDASVA
                     SVDRLIAEAEKSRPELAAVRARAQAAHARVTSARSAGLPTLSASAFAGRNYYDFFGES
                     VPYNTNYSLGILLRVPLFTGFKTVYDTRSAEAAARAADAGAETYAQQVIFQVWNAYFG
                     VQTSAQQVRAAKDLLASARESAEVARGRYREGVGSILDLLTAESALASALAQDVRSRA
                     GFLLAVAQLAHDAGTADTALIPVPERKEAP"
     gene            15459..17642
                     /locus_tag="PLB01_01805"
     CDS             15459..17642
                     /locus_tag="PLB01_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQQ76061.1"
                     /translation="MRRLLLVLAAPFFLAGCGATGPMPTLEDYSQGVSGTVRGIGEYF
                     FEGEESSRLAPAGLGNGEGAGALVDLSRQWRRTCASRCASGLRIVDLQKKSSSALTVR
                     LEVRLEGWRDPERVYALAFFELDFGRVGGGESWTLRASRAVNGPAPIQRGIPHLFEEA
                     AARGLGVANESLDPVEATNLCIPATHHHPGILLVDVDGDGAVDVIVPGRRPRLFLNDG
                     LGRFRDATAGSGLDRLPEMEASGGVAADIDGDGSPDLFLTNHVSPCRMLKNVGGGKFR
                     DVTSEWGLAGLSAPYTSAVFFDADRDGRVDLFVACYGDARSTGPSYRGLNGVGDRFFR
                     NVDRDGHPFFVDETAASGLGDTGWGFAASACDVDDDGDDDLYVANDFGVNSFFENRST
                     PGRPRFVNIAKTAGVEDEGYGMGCTWGDYDGDGRWDLHVTDYWTPFQWILRDARWPMP
                     AMPGAFLARPWMAKIMTRRTRGDGLFRNLGGMRFERTSERSGVADGGWAWGTEFVDLD
                     GKGREDLLVVNGMFRATTGKDDEVGFWNAMGREGVNFHDGVWGGIDFGVNGMASHTPK
                     KLFWNRGDGTFEERSWVEGFDTLEDTRGLAYADLDGDGAPEVVVSCFRGPLLVYANRW
                     GADGGGRVALRLSTPAGFNRDALGAVVRLYAGGRVQLREVRAGSSLMSQSSHDLLFGL
                     GAAGKAERIEIRWPDGRRDTLTDVPAGSLVTLAEGREEKREFLRR"
     gene            17725..20973
                     /locus_tag="PLB01_01810"
     CDS             17725..20973
                     /locus_tag="PLB01_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQQ76062.1"
                     /translation="MKDLFERRTAQSALLVCCALTASSLSFAETPPLRFSSEQASKIL
                     SLRNVGLAQLEEDKAKDARATFAKLAELVPGEALPLANGAVAALREKDLAGAEALLAK
                     AGDRADVWAIRAALENERNRPAAMRSALEKAAALDARDLESRWRYARSVDSDPAAGDA
                     EKVKRRVYLSEIAQRSPANLPARLKLLLLEVEAGDAAAAKKTLGELEPLVSDGDARAR
                     QFLSEARDALGRGDLKTAGVKVRVLENVQRVTPRYQQSLGEVFTQVVGLPVERFSSKV
                     EEGLRASAGPSIPVSLRAPVASEENLERLLRKVDLANSGSPGAYTVPAPFTAAAFFDY
                     DLDGDLDVYLFGASKSDQLLRNNLDGTFTDVTAKTGDAAIQSKKAVVSDVDRDGDLDL
                     VTIDVKGELVLRVNLRQGVFRTVPLRVFGATDLAVEDVNGDGIPDILVGTAKGLRLLI
                     GKGDGSFLEGADNGLGGGAAPAAMRAVALGDFDNDGFPDLIAASSDGLRAWRNQGGGK
                     FAEWAVFSSPSKKSSLPISPDITNADSLLAYDGDRDGDLDLLVSAGGKSSVLVNDGGN
                     ANGWLTVVLEGLATGSGKVNRGGVGSLVEVKAGNLVVTRPAGLWPVHVGLGVRTKADV
                     VRATWTNGVPQNLFDQKAKTVVKEVQQLKGSCPFVYALDGRTGVWSFVSDALGRAPIG
                     LLYDGIHLAGADTREWLLVPEGMLAPTPDGRLLLDYTEELWEAAYLDEVSLMAVDHPR
                     GTSVVPNERMIPGTTEKKLFTVVAPRPVRSAWSDASGRSEDVTERLARADKVYVDPGP
                     ETTYQGVRREHELVLDLGPVAAGSRVVLFLSGWIFYTDTSINVSLSQRKDMVHFPPVL
                     EVPDGKGGWKVAMESFGFPAGKTKTMPVDLTGLIDPSDPRVRIRTTMAIFWDEAYVTV
                     DDPAVAVVTTALPPAKATLSERGFSRRFRETPDGPEVFDHGDTSAAPAWEDVPGRITR
                     LGDVTELLSKTDDRWVVFKGGDAIRIEYDATKLPPLPAGWKRDFVVVSDGWDKDFDKN
                     TVTGQSYGPWPFHAMSAYPYPDAERHPDPKFLEETLTREVGPERFRRGLGER"
     gene            20970..22760
                     /locus_tag="PLB01_01815"
     CDS             20970..22760
                     /locus_tag="PLB01_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQQ76063.1"
                     /translation="MKEDLLRKVNARGAPAAAAVLAALLIAPPLSLSFAEESSSSSSS
                     SSSSLPRHPIRFTDVTKKSGVAFAYDTDLRRGRNLATMGAGVAMGDFNGDSFPDLFFT
                     GSASNGKKPDAGPCGALYRNRGDGTFEDVTARSGIRSCGWRMGAYWVDLDGEGRLDLV
                     VTGVGRVEIWKNLGNGTFRDVAAERGLVGPGYSVGLAAGDVNGDGRVDLYVVGYLDTD
                     YAKETSFGQFEVRVPEDYEGQEAKLNVQGADGRFGERAKEAGVLNTGGRGLSAVFFDY
                     DGDGRPDLYVANDRTTNVLYRNRGDGTFEDVTVEAGAGRRDHKEARAGMGVAIGDLDG
                     KGRPDIFVTNFAGEPKTLYRNVEGALFDDATEASGVERPSLPYVQWGTDIVDLDDDGR
                     PDLVMVSGHLIPKMIMSLARLFGNSKSLGPYTHGDRSYKQAPVLYRNAGGGRMEDATP
                     SSGDLGLLRLSARGLAAGDVDGDGRVDLVIAAISGGTHVLRNATAAPGNALDVLPVAG
                     GDRRTVLGTKIVVTAGGVRQVQEFILRPSYASGAWLPLHFGLGAAETATVEVIPPGAT
                     EPAAKFENVAAGRLYVLRDGALTERRAFRR"
     gene            22757..23311
                     /locus_tag="PLB01_01820"
     CDS             22757..23311
                     /locus_tag="PLB01_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQQ76064.1"
                     /translation="MSAPRVFAFHKPRGVVCSTVAEGGARPVTDLLPPEFRAWFAVGR
                     LDKESEGLLLFSDDAVLAQRLMDPGGVAKVYEVVVEGFPDDEALAPMRAGGTEFDGRT
                     LRPVEVTRVGKAPRGGTRFRVVLHEGVNREIRRLFRAAGHKVRRLRRVAVGPVALGEM
                     RPGEGREISSSERERLQAALAAAR"
     gene            23379..24422
                     /locus_tag="PLB01_01825"
     CDS             23379..24422
                     /locus_tag="PLB01_01825"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQQ76065.1"
                     /translation="MKRTTRVLLALVPALLVFVPPAAAQMQIKTPDASPAATVSQAIG
                     MADFTVSYHRPAVNKREVWGKLVPYGQVWRAGANENTTLTATAPFTFGGKAVPAGTYG
                     VHVLPTADEWTFILSSQSKAWGSFSYDQKEDVVRAAVKPAAGDFTERLAWSFDEPTSD
                     GATLTVRWEKVRASVPIAIDAKAVALANIRDQLRGLPRFGWQGWNGAAAWCLRNKTNL
                     DEAMTWADRSVSMTPTYQNLRTKAGLLELKGDTAGAAEARKKAQALATEADINNQGYQ
                     LLGEGKIDDAIAVFRKNAADHPSSWNVWDSLAEGLENKGDKAGAVENYTKALSMAPED
                     QKKRITDTLARLK"
     gene            complement(24415..25089)
                     /locus_tag="PLB01_01830"
     CDS             complement(24415..25089)
                     /locus_tag="PLB01_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ76066.1"
                     /translation="MRSAIVAGASGLVGERVLRRLLAGGTHDRVIAFVRGPVNITHRK
                     LEQRTVDYERMGRMSAFPRAEDVFCCLGTTIRKAGSPEAFHKVDVGYVARLAEVSVRA
                     GAAQFLLVSSVGASAQSGNFYLRCKGEAEDMVRTLPYRGVQIFRPSFLIGHRRERRAG
                     EAAGIALVRLVSVAMLGPAARYRPIRADDVAKAMVAVARDSPPGIHVYETPAMQALAE
                     DDAAFI"
     gene            25123..26688
                     /locus_tag="PLB01_01835"
     CDS             25123..26688
                     /locus_tag="PLB01_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HQQ76067.1"
                     /translation="MQTVETILTLLGLTTLLAFLAQKAKVPYPIFLVIGGMGLAFVPG
                     LPAVSLDPQLVFLFFLPPILYAAGYFTSWRDFRANLFSISLLAVGLVVATTAAVAAVV
                     VALVPGVPWPAALVLGAIVSPPDAVAATAIAQRVRLPRQIVTILEGESLVNDATGLVL
                     LKFASAAIVTGHLAVLPGLAEFCVMVVGGVAVGLAFGWLFAQTQRYLDDTILATTASL
                     LVSFVTYIAAEHVHVSGVLATVAAGVVQGRVVPRISSSKMRIESTSVWQTFIFLLNAL
                     VFVLIGLQLPAVLDTLDWPWPVVVDGIFATFGTMVVVRLAWMFPGAYLPRYFFPKFRA
                     AHPYPPWQAVVIVGWSGMRGVVSLAAALSLPLVLADGRPFPARSLIIFITFAVILGTL
                     VFQGLTLAPLVRWLRVEEDRSGEKEERRARLALAEAALDHLDGVMDERNLQAHQVHGV
                     RQEYAQRVRAMKGLEGEGPAHAPLELSKELRRTAIEAQRKQLLKLRDEEVIGDDVLHR
                     LQHELDLEDVRMR"
     gene            complement(26695..28437)
                     /locus_tag="PLB01_01840"
     CDS             complement(26695..28437)
                     /locus_tag="PLB01_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQQ76068.1"
                     /translation="MTIQRHFPVLLTLALAACGKPAPKPVSPTPAPEPTKVASSIRFE
                     DVTKASGVSFVHVNGAAGKKWMPETMGSGVAAFDADGDGRVDLLFVNGRWWPGDPRAA
                     QQPTLSFWKNVTEPGSAMKFEDRTKDAGLAISLYGMGVAVGDVNDDGTPDLLITGLDD
                     IRLLVNDGKGRFRDATKGSGLAGPGWGTSAAFADFDGDGVLDLFVGQYVNWTPKTDMH
                     CSLDGKTKSYCTPERYNGVSSRLYKGLGAGKFREITKEAGLVNPLGKALGVAVRDFDG
                     DGRPDLVVANDTSPNNLCRNVETVNGIPHFKDMAIEAGVAVGEDGRARGAMGVAFGDT
                     KNDGGVTLVVGNFSNEMWSVWSAGPKGDFFVDESVQSGIGRTSLLPLTFGVAFADFDL
                     DGVLDLVGVNGHVETSVQDVQPTVTYRQAPLLFRGTGAGRFADVTSQAGPAFAVPMVG
                     RGLAVADLDGDGKPDVVITENGGPAHVYRNVSEGAGKAVRLVLATNGRNRGAVGARVT
                     CTMGGKKRIDEVSGGDGYLSVSERTITIGLGAAAKLDAVDVRWPDGTTASAKDLAPGL
                     YKWVEGGAPEPAKR"
     gene            28494..31199
                     /locus_tag="PLB01_01845"
     CDS             28494..31199
                     /locus_tag="PLB01_01845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQQ76069.1"
                     /translation="MRTARPLPFKEILLLSLSFVFATAAAAGDAPAPPKLRLPAGVAP
                     VRYAAELWLDPAKETFRGKMEIRISLKGESDTLWLNGTELTIESASAVAAGGKDAIAA
                     EGSAVGADYVRIHFQRNLPPGEYDLNLAYTGRVEGKDTQGIFRQKDGADWYAFSHFEA
                     IYARRAFPCFDEPSFKTPWQLTIHAPKGAIAVANTPVVAERSDGDGARAFVFAPTKPL
                     PSYLVAFGVGPFDVVPAGTAGRGKTAIRMIVPKGRAADARWAVESTGPILEVLENYFG
                     IPYPYEKLDHLVIPQTVGFGAMENAGLVTYSSSLLLAKPSDETIRFRRAYASVCAHET
                     AHQWFGDYVTTAWWDDIWLNEAFATWMASKIVDTWKPEWSWAVQRASSRTGAMDQDSL
                     VTARRIRQPIAGNDDIVSAFDGITYQKGAAVIAMFEGWVGEEGFRKGIQHYLKTHAWG
                     AATSDDFVAAIAEATKNPAVVPAFRSFLDQPGVPLVTAELVCAGGAPRLILSQKRFLP
                     TGSTGSKEETWRVPVCARTGDPGAPTTCTLLTEPAGTLALPGACPSRVLANAGSGYYR
                     VLYKGSLLGKVLTDGGKHLTASERIATLSDVAALARSGDVPMATALALVPAFASDPDR
                     PVVESVQRIAAAPRDLLVTDGMRPRYRRFVSDVFGARAKSLGWTPRKGEDEDTQLLRA
                     SLVPFVANEGDEPALVAEAGLLAKAWLKDRSAVDSLLASEVLDVAARHGDVELFQAYL
                     AEAKKAQERRERARLLGALGLFRDPSVVPAALKLTLSPDFDARETIGILREAASSRET
                     RPEAWAFLKANFDRIAARLPRESPARFPMLAAGFSDAEKRADVEAFFKDKAPKYMGGP
                     RYLAQSLEQIQLRGAFKAAQQESVDGFLMHYEPRPTIDLKPQSGM"
     gene            31201..31575
                     /locus_tag="PLB01_01850"
     CDS             31201..31575
                     /locus_tag="PLB01_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76070.1"
                     /translation="MPTGTRRRAPTWESEALGVLAGEFPFSNRAVTEAKIRGRLKRKR
                     LGGYDAARVAVLRRFKDALQREIAKGEASRFYQGPHGRYVDVRDFDVARLRKEMSRRH
                     PRVPKEEVSWFVPFAVFLYHVL"
     gene            31616..32134
                     /locus_tag="PLB01_01855"
     CDS             31616..32134
                     /locus_tag="PLB01_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQQ76071.1"
                     /translation="MKASVFVGTSVDGFLARLDGSFDFLGDGGEPHGYEEFMATVDAL
                     VIGRNTFDVVQKFPDWPYGTKPVFVLSRRALPPVPPGAVVERMAGEPVEIAALLSARG
                     ISHAYVDGGITVQRFLRAGLVQRLVVTRVPVLIGTGIPLFGAVPRDILLKHVATRSYP
                     SGLVQSEYEVVV"
     gene            complement(32157..33047)
                     /locus_tag="PLB01_01860"
     CDS             complement(32157..33047)
                     /locus_tag="PLB01_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ76072.1"
                     /translation="MKIHTLDLEFFRGETIAAYLVESGGRLALIETGPDSTWPHLVAA
                     LARHGARPEDVKDVLVTHIHLDHAGAAWRLARAGANVWVHPRGAPHMADPSKLLASAK
                     RIYKEQMDTLWGTLEAIPAERIKVTSDGLDVPVGDTRIRVLETPGHAIHHNAYLLDGN
                     VFTGDVGGVAIGDGPNLPPTPPPDIDLPTWARSIDRIKAARPDAFYPTHFGRHGDVAR
                     RLDEMMEELHAWAGFIRARLDEGKDEPAIVPIFEAWLTDRLLATGVGAGGLEAYKTAL
                     PFAMNVTGLVRYWKTLGSAA"
     gene            complement(33047..35845)
                     /locus_tag="PLB01_01865"
     CDS             complement(33047..35845)
                     /locus_tag="PLB01_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ76073.1"
                     /translation="MNTRNEGAVRAKGVLHPWQKRLYTILLVPLGLMAANSIYLVAFT
                     KYSSFFMAMLLMHLVLGLLIAIPFLVFAFTHGARMLANIRNRNAKIAGITIVCLSLLC
                     IGTGVTMALKGATLTNRPIYLAHVFAIPAALVAFILHRRAAVHKLHFRMLAQWGGAVA
                     AFLLVMAGLHKMETPPQRIVNKNGDTQFFLSSAETFDQGLLDGKRLSANAYCQDCHPD
                     SFKRWEKSAHRFSSFNNPFYRRSVELMADRVGREKTKWCSGCHDPVVLFTGQMGKATM
                     ATFSYDQFEGQQGLTCMSCHSIAEVKDLRGNGGYVIEESKQYPFAFSDNKMLKEVNKL
                     LIRMEPSLHRQTFMKPFMRTPEFCSTCHKVGLLPPLNDYRWMRGQNHYDSWYDSGVSG
                     NAVRSFYDPPKTKACRDCHLPLMPSKEFGNVAGMLHDHLFPAANTALPALRGDKETIE
                     DIRKKVLVGSLSIDVFALKRGSEIIPLGPSLPSLHPGETVDVEVVVRTRTLGHPYTNG
                     TSDSNETWVEFKAANGPRSVMASGTLDANGRLDPAADKIAQLAIAHDGSFMDRRQPQD
                     IHVPLYNNFIPPGAARTVHYRFTVPKDAAGSLTLAAALNYRKFSRDYSIFVGGPNAPE
                     LPVTRISADSVTLPVAPAPAATLANDVKRGNPDWPEKQWIRWNDYGIGLLLQGDLKGA
                     SAAFKKVAELAGDKPDGPLNLARCKVQEGDLPAAKAALAEAEKRRPGWGKTQYFRSLV
                     SKEEGRLDDALADLDRVLAKFPKDRVVLNQKARVLYLAGRYTDALPPIDAVLAIDGED
                     LAAHYNAMLCLKALGRTEEAAAEEKWYRFFKDDESARALLAEYRIAHPLDNRESLPVH
                     VHDDAVPPPAQPPTWLAEIGPKGYAYKGYAPKGEMVLKDDRPQGAPRPFARPEPPAKQ
                     AGERKKGPGVPVAAAIAP"
     gene            complement(35857..36405)
                     /locus_tag="PLB01_01870"
     CDS             complement(35857..36405)
                     /locus_tag="PLB01_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76074.1"
                     /translation="MTSHDCLCDAFTRDWITSTADGVRPPTWRPLSEHPDARFGPDLE
                     VRRRADLVLAWCTACGRRWYLSWDVKDDCYHPIPLGSEMLDVLNLDIPLPRLAAFLLG
                     PAYGTYSWALEGFVDRFLWEGAYDAADAEARLREALATAGLKGAVRSSLERRLTSVLE
                     REKRRAWAARPRRRRPENDSQR"
     gene            complement(36402..37013)
                     /locus_tag="PLB01_01875"
     CDS             complement(36402..37013)
                     /locus_tag="PLB01_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76075.1"
                     /translation="MRTRPAAFALLAALVATGCASSDWAKTQSVPGGKLYIPKLYAPL
                     LPGSIYPDKKVPLPAKGRPALVVACPAKGDCRKDVILDQAAHRGFVVLVGREPKDDLL
                     RTRVEADAERIGWLLVRPDEDFLRRWTRAGAQGAATAVIGPPPFPSYPSKHLLFAALH
                     SAEPPEARDGMVLKLYSPNEKGLLPNEAFRDAVEWLAGELDSK"
     gene            complement(37010..>37678)
                     /locus_tag="PLB01_01880"
     CDS             complement(37010..>37678)
                     /locus_tag="PLB01_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQQ76076.1"
                     /translation="TVTALAKKEGPRAPSPAEEARGTAWGRVLHQLLEAAMRTPGAPL
                     APLAQNLLREEEVAPDLLGEVLRVAESVTASDLWARALKAPRRYVEVPFEMVVPSKDL
                     GLEAGPSETLLKGAMDLVFEEDGVWRIVDWKSDVVGDGLAALVAHYAPQVAHYRRAWE
                     ALTKKPANAGLFFMDTGELVWLGMEGNEREEKISLKVNTTDREAGARQDGSAPRQRSL
                     FEES"
BASE COUNT         5580 a        12829 c        13529 g         5740 t
ORIGIN      
        1 cggccatcgc cggccccttc tggtcgccgg tcaccatgac cgtccggatg ccggcgaggc
       61 gaagctcctc gatcgcgccc cggacgttcg cgcgcggcgg gtcgatgaag ccgacgagcc
      121 ccacccacgt gagcccttcc tcgccggcct cggcgtcgtc gtcggcgccg agggcccggc
      181 acgcgaacgc gaggacgcgg tggccggacg cgccgagggc gtgagcggag gcgagcaggc
      241 gctcgcggcc ggccgcgtcg agcggcacct ccgcgccgcc ggagaggatg cgagcggcgc
      301 gcgtggcgag gctctccggc gaacccttga cgaaggacca gatccggcct tcaccgtcgc
      361 gcgccaggac ggacatgcgc tttcgctcgg acgagaacgc gaccgcgctc aggcgctcga
      421 agcgcgatcc gtcgacgccc cccttggccg cgagagcgag gagcgcgccc tccgtggaag
      481 tgccgtggac gtgccagccc tcgtgggact cgagcgtcgc gtcgttgcag acggcagcga
      541 tctcgagtag acgcgtgagg tcgtctcccg gcggcgcggg cctgccggct tcgaggaatt
      601 ctccggccgg atcgtagcgc tcgccggtga cgtcgaggct gcgcccgggg agcacgacgc
      661 gcgtcgcgcg catgacgttc tccgtgagcg tgcccgtctt gtccgtgcag atcacggacg
      721 cgcagcccag cgtctcgacc gatgagagcc gcctcacgag gctgtccgcc ttcgccatcc
      781 gccggacgcc ggcggccagc gcgatcgtcg tgacggcggg gaggccctcg gggatggcgg
      841 cgatcgcgag gatgagcccg gtctcgagca tgagccagaa ggggcgggcg cgcaggaagc
      901 cgaggaccgt cacgagggcc gagagcccga tggcgaggag cgccagcctc cggccgagct
      961 gctcggcctg cttttcgagg ggagtctcct ggcgcccctg gtccgcgacg agctttccga
     1021 tgcgcccgag ctccgtgccg ccgccgatcc cggtcaccaa aatacggccc gaaccgctca
     1081 cgaccgtggt gccggcccac gccatcgacg tccgctcggg cagaggggcg gcggcctcca
     1141 ccggttccgg cgacttcgcg acggggacgc tctcgccggt gagcgcggac tcgtcggcct
     1201 gaaggtcgtt ggcgaccgcg acgcgtccgt ccgcggcgac gatctggccg cccatgagca
     1261 cgaggagatc gcccggccgg aggtcctcgg ccgggatctc ccgctcgagg ccgccgcgca
     1321 cgacgcgcgc ggtcggagcg gagaggttcc gtagcgcctc gagggcgcgc tcggcgcgga
     1381 agtctgtgac gaacccgaag acgaccgaga agaccacgga agccccgatc gcgagcgcgt
     1441 cgccgacgtg gccgagcgcg agcatcaccg cggtcgccgc gatcaggacg agaatgacgc
     1501 cgcccttgag ctggttgcca aagatgcgcc aggggccgcg accctccgta tggaccgcat
     1561 cgtcgggcgg gcgctcggcg aggcggctgg gcggcaggcc gagctcgacg tgcgttccga
     1621 gagcggcggc gacgtcgtcc acgtgccgcg cgtgccaggc ggcggccgga ggagacgcag
     1681 gcgctcgagt cggctcgcga tcgctcatag gcgggcgccg aatccggacg ccctccgtcg
     1741 agcgtactcc cggggcgaga ggtgccgccg aatgagacat ttgcatcatg gatgcagtct
     1801 caagaaaggt ggagcataat ggcgcaggat gagacttagt ctcaaccaga aagcgcatcc
     1861 gatgcccgct tcagccgccg ccgccgtctc gaccctggcc gacctcgtgc cggggcagag
     1921 cggcgtcctg accctcctcg accttcccga aggggacgcc cagcgtctca tggagctcgg
     1981 cttcctcccc gggagcgtga tcgtggccgc ccggagcgcc ccgggcgggg acccccgcgt
     2041 ctaccgcgtc gaggggacgg agttcgcgct gcgccgggac acggcacggc acattcacgt
     2101 cgagggctcg tccatcggcc gcgcggctgg ggaaggccgg tgagcgccgc atcacagtgc
     2161 cacgggtgcc cggtcgcggc ggcgcccatt gccgtccacg gccaccccca cccgcgcctc
     2221 gtgggcctcg tcggtccccc gaactcgggc aagtcgacgc tcttcaaccg gcttaccggc
     2281 ttgaggcaga aagtcgcgaa ctacccgggc gtcacggtcg aggaacggcg cggccgcgcg
     2341 cgcctcgacg gcgacaccga ggtcgttctc atcgatctgc ccggcgtcta cagcctgacc
     2401 ccgcggtcgg aggacgagcg cgtcacccgc gacgtcctcg cgggcgagat ggcgggacag
     2461 cagaagcccg acgcgattct cctcattctc gattcgacga acctcaagcg gcacctcgtc
     2521 ctcgcggcgc ctgtcctcgc actgggcctt ccgacgctcg tcgtcctgaa catggcggac
     2581 gagctcgaag cacgcggagg ctccgtggac cccgcggctc tggccggaca gctcggcgcg
     2641 cccgtcgcgc tcgtgagcgc ggccaagggc gaggggatcg agacggtcta cgactttctc
     2701 aacggcgccg tgggcgtccc gcgggcgatg gacctgccca cgctcgcgga catcccgaag
     2761 tgccgtcagt gggccgtccg cgtgggcgac caggccgggt acgcggcgcc ggcgccgccg
     2821 gtgtggacgc gccgcctcga cgccgtcttc ctgcatcccg tcgctggtcc gctcatcttc
     2881 ctcgccgtcg tcgtcgcggt cttccagacg atcttctcgg cggcacgccc tctcatggat
     2941 ctcgtcgatg ccgccgtgcg cgcctccggc gcgtgggtcg gcgcactgct cccggcgtcg
     3001 gccatgcgta gcctcctcgt cgagggagtc tggggaggcg tcgggtcggt cctcgtgttc
     3061 ctcccgcaga tcctcctcct gttcctcttc atcggacttc tcgaggactc gggctacctc
     3121 gcgcgggcgg cgctcatcgc ggaccgcacg atgcgcaagg ccggcctgca gggaaagtcg
     3181 ttcctgccgc tcctctccgc ctacgcctgt gcggttccgg ccgttctcgc ctcccggacg
     3241 atcgagaaca agcgcgaccg gatcgcgacg atcctcattg cgccgttcat gacgtgctcg
     3301 gcgcgcctgc cggtctacac gctgatcatc gcggccttca tcccggaacg cgccctcctc
     3361 ggaccgttcc tcgggacgcg cgcggcggcg atgctcggcc tgtacgtcct cggcttcctc
     3421 gccgccttcg ggacggcgcg tctcctgaaa tcgtcggtcc tcaagagcga cggcgcgccg
     3481 ttccttctcg agatgccgcc gtaccgccgg cccacgctcc ggcagatcgc cctccggatg
     3541 ttcgaccggt cgaagatctt cctgcgccgg gcgggcaccg tgatcctcgg cgtcgcgatc
     3601 gtcctctggg tcctcgcgca cctcccgctt tcggcgggcc agatgcccga gatcggaaac
     3661 agcgtggcgg gcacgctggg ccgcgcgctc gagcctctcg tgaggccgct gggattcgac
     3721 tggcggatcg cgatcggcct cgtgacgtcg ctcgccgcgc gcgaggtcat cgtcgggacc
     3781 ctgggcactc tctacgggat ggacgcccac tccggtacgg cgggtctcca ggcggccctg
     3841 cggcacgacc tgacgccggg cggcgcggtc gcgctcctcg tcttcttcgc gttcgcgatg
     3901 cagtgcctct cgaccgtggc ggtcgtgcga cgcgagaccg gcggctggcg gtggccggcg
     3961 atccagttcg cctacatgag cctcctcgcg tactcgggcg ctttcgtcgc gaatcgggtc
     4021 gtgacggccc tcctgggctg agacgcggtc gcacaaaacg ggtatcctgc cgccgggtga
     4081 accggaagaa gttcttcgac gcagcggccc actgggacgt cgaaacggtt tcggcgggcc
     4141 tccgggagga ccggggtctc gcccgggtcg tggacgagcg cgggcgcacc gccctgcacc
     4201 gctgcgcgcg gacgaacggg gagaaggacg ggcgggacgt cgcggcctcc ttgcgcgtcg
     4261 cgaaggcgct cctgaaggcg ggcgcgaatc ccgacgccgt tcacgacatc cccaacgagg
     4321 gcggaagctt caaggccacg cctctctggt acgcggtcgc gtggggacgg aacccgaggc
     4381 tggtgcagct cctcctcgac gacggcgcgt cgccgcgggg ttgcctctgg gcctgcgtgt
     4441 gggcgaacga cgtcgaaatg gcgaagacgc tcgtgaaagc gggcgcgcct ctgaacgaag
     4501 cgtccggagg cgaaacgccc ctcgcgtacg ccacgcgcct caggaggccg gcgttctaca
     4561 ggatcctcgg cggacgttga cgtccggccc cggtcatgcc gcggcgaagc tgttgcgcgg
     4621 ctcgacctcc ttggcggcct gggcctgact cgcggcgcac atcgtctcgc agaacatccc
     4681 gagttcctcc aggacggcgc aggcctcggt cgcacagacc gggcagcgcg tgcaagtgtg
     4741 atgaccctcg gggttcaggc tcgagccgga ctccgggagg gtcgggctcg gcttttgagc
     4801 tgggctcatg ggcacctccc tgaaggacaa tatagggcga tttacgcatt gcgtcaactc
     4861 gatgcgtcac acgccgaggc gaccgatccg ctggcggtcg agggcccgcg ccgactgccg
     4921 gccgaggatc cggacgccgc cgagcaacgc ggcgaagcgc gggtccgtcg tgtgcccctc
     4981 ggcgaagcgg cggtagatct gctgggcgat gacggcgatc ttgaagaggc cgaagacgta
     5041 cgcgaagagg acgtcggcgt cgggacgccc cgtccgctcc ttccaccggg cggcgacctc
     5101 ctcgcgcgac aggttcccgg ggatcagcgt cggaccgaac ggcagcgccc ggacctcggg
     5161 cgcgtcgtcg gggtcgatcc agtagccgag cgtcgtaccc acgtcgagca ccggatcccc
     5221 gagcgtcgcc atctcccagt cgagaacggc gagtatccgc cgcggatccg cagggtcgag
     5281 gacgagattg tcgtacttga agtcgttgtg gacgagcgcg gaacgcgccg cctcgggaac
     5341 gcgcccggcg agccatgcgg cggccgcgtc gagcgcaggc acgtcctccg tcctcgcacg
     5401 gatccagcgc tccgtccagc cggagacctg ccgggcggcg tagccctccg gcttgcccag
     5461 cgcggcgagc gggcccgtgg aaacgtcgag ggcgtggagc tccgcgaggg agtcgacgaa
     5521 cgcctccgag agcgcccgca ttcgcggggg cggcaggtgg acgccggggg gcggctccga
     5581 cgaacggaga acgagaccct ggacccgctc catgagaaag aaaggagcac cgatcaccga
     5641 ggggtcgtcg cagaacgcga gggccttcgg agccttcgaa tagaggggag agacggcgga
     5701 caggacgtcg aactcgcggc gcatgtcgtg ggcggacttc acggaagcgc cgtgcggggg
     5761 gcgtcgaaga acggcctcgc gcccgccgaa cgtcacgaga tacgtcaggt tcgagtggcc
     5821 ggcgccgaac tgcgaaacgg aaaccgtcct ggcgccgcca cccgctgcac cgaaatcacc
     5881 cggaaacgcg cgtgcgatga actccgcgag ggcaccttca tcgaggcggt cttctcttcg
     5941 gacctcccgg ggcgcgtcgg ggtcgctcat ccgcggtgcg gaagcccgaa tcgcctgagc
     6001 gtgtgccgcc cgacgaagga aaggtggacc tcgtccggtc cgtcgtagat gcgcgcggcg
     6061 cgttcatggg cgtaccagaa cgcgagcggc gtgtcgtccg tcattccgag gccgcccagc
     6121 gtctgcacgg cgcggtcgag gacgcgctgg agagcgcgtg cggcggtgaa cttgacgagg
     6181 gagatctcct cgcgcgcggc cttcgggccg tccttctcca ggagccacgc ggcgtggagg
     6241 acgacgaggc gcgtcgcgtg aatgtcggcg cggctctcgg cgacggcggc ctcgacgagc
     6301 tgccgcgtcc cgagcggcgt ccccggcgcg atctcccgcg ccacggcgtg gcggcacatg
     6361 atctcgaggg cgcgctccat gacgccgatc caccgcatcg cgtggtggat ccggccgggt
     6421 ccgagccgct cctgcgcgag cgcgaagccg tctccctcgc tcccgaggag attcgcgagc
     6481 ggcacccgga cgccctcgta accgacctcc gcgtggctcg cccagccgcc gccgcggtgg
     6541 cccatcaccg agaggttccg caaaatcgaa aagcccgccg tatcggtcgg gacgaggatc
     6601 aggctcgcgc gccggtgggg cgcggcgtcg ggatccgtga cggccatgca gaccgcaaag
     6661 gcggctccct cggcggacga cgcgaaccac ttgtggccgc ggatgaccca gtcgccgccg
     6721 tcccgcttcg cggtcgtgcc catccacacg gggttcgagc ccgcgaactc gggttccgtc
     6781 atcgtgaaac agctccggat ctcgccggcg acgagcgggc gcagccagtg ttccttctgt
     6841 tcgtcggtgc catgcccgag aaggagctcc atgttcccga cgtcgggcgc ctgcacgttg
     6901 aaggcgtagg ggccgagcgg ggaacggccc agctcttcgc tcatgtgtgc gaactccacg
     6961 agggagagtc ctgcgccgcc ccacgcggac ggaaggtgcg ccgcgaagag tccggtgccg
     7021 cgcgcccgtc gccggacggc ctcgagcgcc ggcgcgactg ccggccagcc ttcgttcagg
     7081 aacttcggct cgagcggaac gacctccttt tcgacgaagg cccgcacgag ttgacgaagt
     7141 tgtttcacgt tttcgggcgc ggaaaaatcc acggaccctc cgatcggcgg cctgttccgt
     7201 tgtattctga ccccggccga cctccaggcc ggaaaggcct ttatgtcggt acttccgaag
     7261 atcatccagg gcggcatggg cgccggcgtg tcgaactgga cgctcgcaaa cgcggtgtcg
     7321 cggctcgggc agttgggcgt cgtctcgggc acggcgctcg acgtgattct cgcccggcgt
     7381 ctgcaggacg gcgatccggg cggccacatg cgccgcgcga tcgccgcgtt tccgtttccc
     7441 gagatggcgg gccgcgtcct caggaagtac ttcgtcgacg gagggaggcc cgaaggcgcc
     7501 ccctaccggg cgatcccgat gtacgcacgc acggggtcgg cccgcgagct cgtggagctt
     7561 tgcatcgtcg cgaatttcgt cgaagtctgg ctcgcgcgcg aaggccacga cgggcccgtc
     7621 gggatcaact acctcgagaa gatccagctg cccgtcctgc cgtcgatcta cggcgcgatg
     7681 ctggcgggcg tcgccgcggt cctgatgggc gcggggatcc cgatccgcgt ccccggaatc
     7741 ctcgaccatt tcgtcctcca cgaggcggcc gagtatcccc tcgcgatcac gggatcgatc
     7801 gagggcgacg acacggcgat gcgcttttcg cccgccgact ggatcgaggg cgcggccccg
     7861 gcgctgaaga ggcccctgtt cctcccgatc atcgcctcga acacgctcgc gctgacgctc
     7921 cagcgcaagg cgaacgggcg cgtggacgga ttcatcatcg agggcccgac ggcgggcggc
     7981 cacaacgcgc cgccgcgcgg caagcccgct ctctcggcga cgggcgagcc cgtgtacggg
     8041 gagcgcgacg tggtggacct cgcgaagatg cgcgagatgg ggctgccgtt ctggctggcc
     8101 ggcgggcgcg ggacggccga gggactccag gacgcgctcg tgggcggcgc cgcggggatc
     8161 caggtcggaa ccgcgttcgc gtactgcgac gagtcgggcc tcgacgccgc gacgaaagcg
     8221 cgcgtcctcg cactcgccgc gtccggtggt gcgcacgtct tcacggaccc ggtggcgtct
     8281 ccgacgggtt tccccttcaa ggtcgtgcag atcgagggca cgctgtcctc cgaggaggag
     8341 tacctgaagc gcacgcggat ctgcgacctg gggtacctgc gcgagccgta ccgccgggag
     8401 gacggctcga cgggctaccg ctgtgcgtcc gagcccgtcg cccagtacgt cgccaagggc
     8461 ggcggcgagg aatacgcgca gggccgcaag tgcctctgca acgcgctcat ggcggacatc
     8521 gggatcggcc agacgcgcgg gggagtcgtc gagaagacgc ttgtgacgtc cggcgacgac
     8581 gtcgccgaca ttgcccgttt tctgacgtcc ggctcgacgt cctacagcgc ggcggacgtc
     8641 gtccggcggc tgctcgagac ggcctcgtag gccttcagac cttcgcgcgc ccgtagggca
     8701 cgggccactc gaccttcgcg ccgagctcct tcgcggcgtg gagaggccag taggggtcgc
     8761 gcaggaaggc ccgggccatc acgatcgcgt cggcccggcc gttctgaaga atctcctcgg
     8821 cctgcttcgg ctcggtgatc atcccgacgg cgcccgtcgg aatggacgcg tcgcgacgga
     8881 tcgcctcggc gaacggaacc tggtaaccgg ggccgacggc caccttcgcg ttcggtacga
     8941 ggccgccgct cgagcagtcg acgaggtcca ggccgagctc cttcaggcgg cgcgcgaaca
     9001 cgatcgactg agcgagatcc cagccgcctt cgacccagtc cgtcgccgag atccgcacga
     9061 agacgggcct gtcggcgggg aaggccgcgc gcacggcgcc cgcgatgcgc agcgggaatc
     9121 gcatgcggtt ctcgagcgtc ccgccgaagc cgtccttgcg gtggttcgag aggggagaga
     9181 ggaactcgtg caggaggtag ccgtgggccg cgtggacttc caggacgccg aagcccgcgt
     9241 cgacggcacg tcgcgccgcg ccggcaaact cgtccgtcac gcggtccatg tcggcggacg
     9301 tcatttcgcg cggcgccgga acaccggcgt cgaagggaat cgcgctcggg gcgagggtcg
     9361 tccacgctcc ctcgccggcg gccagagggc cgctgcccct ccagggcacg tgcgtcgatg
     9421 ctttgcgtcc cgcgtgcgcg agctgaaggg ccggcacggc gccctgcgcc gagacgaatc
     9481 cggcgatgcg gcggaaggcg tccacctgcg cgccgttcca gagcccggtg tcgccggggg
     9541 agatgcgccc ttccgccgag acgctggtgg cttccgcgat cacgagcgcg gcgccgccca
     9601 ccgcgcggct gccgaggtgc acgaggtgcc agtcgttcgg gacgccgtcc acggcggaat
     9661 acatgcacat cggcgagacg aagatccggt ttcgaaacgt cgtgccgcgc aggcggaggg
     9721 gctcgaagag gcgggccacg agcgtcagcc gcccgcgggc gcaggcgcgt cgaccgcgcc
     9781 cggggaggag gcgtgccggc ggcgcttgat ccagccgtcg aggcggtcca tggtgacgta
     9841 gaagaccggc gtgacgtaga gcgtgatcat ctgggagaag aggagaccgc cgacgaccgc
     9901 caggcccagc ggccggcgcg attcggcgcc ggcgccgaac ccgagcgcga tcggcagcgt
     9961 gcccatgagg gccgccatgg tcgtcatcat gatcgggcgg aaccggacga cgcaggcctc
    10021 gtagatcgcc tcctcggcgc ccttgccgcg gcgccgcgcc tcgacggcga agtcgaccat
    10081 catgattccg ttcttcttga cgaggcccac gagcaggatg acgccgacga acgcgtagat
    10141 cgagagctcc gtccggaaga tgacgagcgt gaggagagcg ccgaagccgg cgaacgggag
    10201 ggccgacagg atcgtgatcg ggtggaggaa gtcctcgtac aggatgccga ggaccgtgta
    10261 gatcacgagg atcgcgacga ggaggaggag cccgaggccc ttctgcgacg cctcgaacgc
    10321 ctgtgccgtt ccctggaagc tcgtctggac gctcgcgggc agcgtcgcgc gcgcgacctt
    10381 gttgatctcg gtgacggcct cgccgagcga gacgccgggc gcgaggttga acgagagcgt
    10441 gaccgcgggc agctggcccg cgtggttcac ggcgagcggg cccaccgtcc gctcgaggtg
    10501 cgtgatcgcc gagagcggga cgagctcgcc cttcttcgag cgcacgtaaa ggagctggaa
    10561 cgcggagggg tcgcgctgga actccgggag gagctccatg atgacctggt actcgttgtt
    10621 cggcgcgtag atcgtggaga tctggcgcga gccgtacgcc gagtagagcg cgttctcgag
    10681 ggactcgggc gtcacgccga gggcggaggc gcggtcgcgg tcgatcgtga ggaacgcggt
    10741 gggattgcgg agctggaggt cgctgttgag gtcctgcagg ccccgcaccc cgcggagctt
    10801 ctcctcgagc gccggcgccg acgcgtagag gtccttcgtg tccgaggcct gcagcgtgaa
    10861 ctggtactgc gcgcgcgtga gcgccccgcc gagacggatc ggagggagca cctgcgggta
    10921 cacgcggatg cccggcacct gcgcgagctt cgggcgcagg cgctggatga tctcgtcgac
    10981 cggcggcctc tcgcggcgcg gtttcagccg gatgaacatc gtgcccgtgt tcccgccgcg
    11041 cacgtcgttg ccgcggttgc cggcgctcga catgaacccg tcgacgcccg gatcctcggc
    11101 catgatcgcg gcgacggctt tctggtgctc gatcatcgac tcgaacgaga tgccctgggc
    11161 cgcctcggtc ggggcgaaga tctggcccgt gtcgtccgag ggcaggaagc ccttcgggat
    11221 gtgcacgaag agccagcccg tcagggcgag gatgacgagg gagaacgcca tcgtggcgcg
    11281 ctggtgggcg agggcccagg tgaggccgcg ctggtagaaa gcgagggcgg agtcgaagac
    11341 gcgctcggag gcctggtaca ggcgcccgtg ccgctcctcc ttcgagtgcg cgcggaggta
    11401 gcgcgcgcag agcatgggcg tgagggtgag cgagacgaag cccgagacga ggatcgcgac
    11461 ggcgatcgtg accgcgaact cgcggaagag ccgcccgatg atcccgccca tgaagagcag
    11521 cgggatgaaa accgccgcga gcgagatcgt catcgagagg atcgtgaagc cgatctcctt
    11581 cgtgccgttc agggccgcct cgaacggctt ctcgcccatc tcgaggtgcc ggacgatgtt
    11641 ctcgagcacg acgatcgcgt cgtcgacgac gaagcccacc gacagcgtga gcgccatcag
    11701 cgagaggttg tccatgctgt atccgaggac gtacatcgcg gcgaacgtgc cggcgatcga
    11761 gagggggagc gcgagcgacg ggatcaccgt cgccgacagg tttcgcagga acaggaagat
    11821 gacgaggacc acgagcgcca gggtgagcca gagcgtgaac ttcacgtcgg cgacggactc
    11881 gcggatggac tgcgaccggt cgaaaaggat gttcagcttc gccgagcccg ggagcgccga
    11941 ctggaactgc gggaggagcc tccggatgtt cgagacgacc tcgaccgtgt tctcgccggg
    12001 ctgcttctgg accgcgagga cgatgctgcg gttggggccg gtcttgtcga cgtaccagga
    12061 cgccgtcttg tcgttctcga cgccgtccac ggcccggccc acgtcccgca cgcgcacggg
    12121 cgcgccgttg cggtacgcga tgatgagcgg cccgtactgc gcggcttcgg tgagctggcc
    12181 cgtcgcctgc accgtgtacg ccgtgttcgg cccccagagc gtcccggtcg ggatgttcac
    12241 gttcgcggac gccacggccg ccgaggcctc gtcgatcccg atcccgcggt acgcgagcgc
    12301 gcgggggtcg agctgcacgc gcacggcgta tttctgcgag ccgaagatca ggacctgggc
    12361 cactcccgag acggtcgaga tccgctgcgc catcatcgtc tcggcgtact cgttcaggac
    12421 cgagagcggg agcgtcggcg tcgtgagcgc gatgtagagg accggctggt ctgccgggtt
    12481 caccttctgg tacgtgggcg gcgtcggcat gcccggcggc agctgccgcg acgcggcgcc
    12541 gatggcggcc tggacgtcct gcgcggccgc gtcgaggctg cggctcaggc tgaactggag
    12601 cgtgatctgc gtgacgccga ggatcgacgt cgacgtcatg ttgtcgaggc cggcgatcgt
    12661 cgtgaactgc ttttcgagcg gcgtcgcgat ggccgaggcc atcgtctcgg ggctggcccc
    12721 gggcagcgcg gcggtcacct ggatcgtcgg gaagtcgacg ttcgggaggt ccgagacggg
    12781 caggagccgg taccccacga tcccgaagaa caggatcgcg agcatcacga gcgtcgtcgc
    12841 gacgggtctc ctgatgaaga gctcggagat attcatttct tgatggtgac cttcgcaccg
    12901 ggcgagaggc gcagctggcc gtccgtgacg acgcgctcgc cgggcgcgag gcccttctcg
    12961 acgaccgtcc actgcttcca cacgcgccgc acggtcacgg ggcgcggctc gacgctctcg
    13021 tccggcttca cgacccacgc gagcgagccc gcctgccccg gttgaacggc ggtgttgggc
    13081 acgacggtcg ccccggcgtc cgtcgtgagg acgagcgaga cgttcgcgaa ctgccccggc
    13141 cacagcgcgc gttcgtggtt cgggaacgtc gccctcagat tgatcgtgcc cgtcgtcggg
    13201 tccacggtgt tgtcgatgaa cgtcaggtcg ccctccgagg cgacggcccc cgggctgttc
    13261 gggagcgccg agaccttgag cgtcccggac gcggcgcgct tcttcacgtc cgcgagcgcc
    13321 gcctccggaa cggagaacgt cacgtagatg ggctcgacct ggttgatgac gacgagcgcg
    13381 cggtcgtcgt tcgccttcac gagattgccg gggtgcacga ggagcccgcc cgtgcggccc
    13441 gagatcggcg ccttgatcgt gcaataggcg acgttcagcc gcgccgtctc gagcgccgct
    13501 tcgtctccct tgaccgttgc gcgcagcgcg tcggcgttcg cggtcgcctg cgagaactgc
    13561 tccttcgtga cgtagtcctt cttgacgagc tcggcgtagc gcttgaggtt ctcgtccgct
    13621 tcgcgggccc gcgcccggtc ccgctcgagg ttggcctcgg cctcggcgag cgccgcccgg
    13681 tagggccgct ggtcgagctc gaagaggagg tccccggcct tcacgtcctg gccttcgcgg
    13741 aaggccacct tcgtgacctc gccgccgacg cgcgtccgca cggccacgtt cgagtacgcg
    13801 tccacgtgcc ccacggccgt cacctcgagg gggacgtcca tcttctcgac cgtcgtcgcc
    13861 gtgacgggga gggacggcgg cgcgggcggc gtgcgcgcgg cgcgcgtgca tccgccgagc
    13921 gcggcggcca ggaggactgc ggggacgatc cgtctcatgg agcttccttc cgttcgggaa
    13981 ccgggatcag ggccgtgtcg gccgtcccgg catcgtgcgc gagctgggcc acggcgagga
    14041 ggaagcccgc gcggctcctc acgtcctgcg cgagggcgct cgcgagcgcg ctctcggcgg
    14101 tgaggaggtc gaggatcgag ccgacgcctt ctcggtagcg cccgcgcgcg acttccgcgg
    14161 actccctcgc gctcgcgagg aggtccttgg cggcgcgcac ctgctgggcc gacgtctgca
    14221 ccccgaagta ggcgttccac acctggaaga tcacctgctg cgcatacgtc tcggcgccgg
    14281 catccgcggc gcgcgccgcc gcctcggcgc tccgcgtgtc gtagacggtc ttgaagccgg
    14341 tgaagagcgg aacgcgcaga aggatgccga gcgagtagtt cgtgttgtag gggacgctct
    14401 cgccgaagaa gtcgtagtag ttgcggccgg cgaaggccga cgccgagagt gtcggaaggc
    14461 cggccgaccg ggcggacgtc acgcgcgcgt gggccgcctg agcccgggcc cgcacggccg
    14521 cgagctcggg gcgactcttc tcggcctccg cgatgaggcg gtcgacggag gcgaccgagg
    14581 cgtcctcgtg cacgtcctcg ggcaaatcgg cggcttcgac cggcaggtcc acggggacgc
    14641 cgagggtcgt cgcgagctgg ccccggaacg tctggatctg tccgctcacc tggtcgtacg
    14701 cgagcttctg ctgcgacacg accgtccggg cctggaggac gtccgcgatc gtcgcgacgc
    14761 cggcgcggtg gcgctcttcg gcggccgcat agttttcgcg cgcctgcttg aggctcgcct
    14821 cctgggcgac gaggagcgcc cgggccccct cgtagaggta gtacgcctgt gcgaccccga
    14881 gaatcaggtc gttcatggcg gcgtcgtgga cgaagttcgc cgcgacgagg cgccgcttcg
    14941 tctcctcgac gtccgcggcg cggcctccga gatcgaggag gagccagttg aggctcacgg
    15001 acggcgcgta cgtcgtcgac agcgtcgtga cggagccgcc gagcgcgctc tgtttctggc
    15061 gggtcccgga gaggtcggcc tcgacgacgg ggtagtacgc cgagcggcgg ctcccgacgt
    15121 ccgccgccgc ggcctgcgtg aggaaccagc tctggcgcgt cacgggactg ttcgcgagcg
    15181 cgacgtcgat cgcctcgtac agcgagatct tcctcttcgc gagcctttcc ggcgacgcct
    15241 ccgggggcgg cggcgggggt ttcggcgcgg gcggaatcgc ctccgccggc gccacccagc
    15301 gggcctcggg agccttcggc gtgtccggcg cggacgcgca cgccccgcaa agcgccgccg
    15361 tgagcatggc ggcggaggtg ctgagggaac gcggcacgac gcgattatgc ctacgggacg
    15421 cggaagagaa ggagtttcat tctggctaag atcccggtgt gcggcggctt cttctcgtcc
    15481 tcgcggcgcc gttcttcctg gccggctgcg gcgcgaccgg gcccatgccg acgctggagg
    15541 actactccca gggagtgtcg ggaacggtcc gggggatcgg agagtatttc ttcgaaggcg
    15601 aagagagttc ccgcctcgcg ccggctggcc tcggaaacgg ggagggcgcc ggtgcactcg
    15661 tcgatctctc gcgccagtgg cgccgcacct gcgcgagtcg ctgcgcgtcg gggctccgga
    15721 tcgtcgacct tcagaagaag tcctcttcgg ccctcacggt gcgcctcgag gtccgcctcg
    15781 aaggctggag agatcccgag cgcgtctacg ctctggcgtt cttcgagctc gacttcggaa
    15841 gggtcggcgg gggcgaaagc tggacccttc gcgcctctcg ggccgtcaac ggccccgctc
    15901 cgatccagcg cgggattccg catcttttcg aggaggccgc ggcccggggc ctcggcgtcg
    15961 cgaacgaatc gctcgacccc gtcgaggcca cgaacctctg catcccggcc acccaccacc
    16021 accccgggat cctcctcgtg gacgtggacg gagacggcgc ggtggacgtg atcgtccccg
    16081 gccggcgccc gcgcctgttc ctcaacgacg ggctcggccg gttccgcgac gcgacggccg
    16141 gctcgggtct cgaccgcctc ccggagatgg aagcctccgg cggcgtggcc gccgacatcg
    16201 acggcgacgg cagccccgac ctcttcctca cgaaccacgt ctcgccctgc cggatgctga
    16261 agaacgtcgg aggcgggaag ttccgcgacg tcacgtccga gtgggggctc gcggggctgt
    16321 cggcgccgta cacgtccgcg gtgttcttcg acgcggaccg cgacggccgc gtggacctct
    16381 tcgtcgcgtg ctatggagac gcgcggtcga cggggccgtc gtatcgcggc ctcaacggcg
    16441 tcggcgaccg tttcttccgg aacgtcgacc gggacggcca tcccttcttc gtggacgaga
    16501 cggccgcgtc gggcctcggc gacacgggat ggggattcgc cgcgtccgcc tgcgacgtcg
    16561 acgacgacgg ggacgacgac ctctacgtcg cgaacgactt cggagtcaat tcgttcttcg
    16621 agaaccgctc cacgccgggc cgtccccgtt tcgtgaacat cgcgaagacc gcgggcgtcg
    16681 aggacgaagg ctacggcatg ggctgcacgt ggggcgacta cgacggcgac ggccggtggg
    16741 acctccacgt cacggattac tggacgccct tccagtggat cctgcgcgac gcgcgctggc
    16801 caatgcccgc gatgccgggc gccttcctcg cgcggccgtg gatggcgaag atcatgacgc
    16861 ggcggacgcg gggcgacggc cttttccgga atctgggcgg gatgaggttc gagaggactt
    16921 ccgagcgctc gggagtcgcc gacggaggct gggcctgggg cacggaattc gtcgacctcg
    16981 acggcaaggg ccgcgaggac ctcctcgtcg tgaacggaat gttccgcgcg acgacgggca
    17041 aggacgacga ggtcggcttc tggaacgcga tgggccggga gggcgtgaac ttccacgacg
    17101 gcgtctgggg cgggatcgac ttcggcgtga acggcatggc gtcccacacg ccgaagaagc
    17161 tgttctggaa ccggggcgac gggacgttcg aggagcggtc ctgggtcgag ggcttcgaca
    17221 cgctcgagga cacgcgcggc ctcgcctacg cggacctcga cggcgacggc gcgcccgagg
    17281 tcgtcgtctc ctgctttcgg gggccgctcc tcgtctacgc gaaccgctgg ggcgccgacg
    17341 gcggcgggcg ggtcgccctg cgcctctcga cgcccgcggg cttcaaccgc gacgcgctcg
    17401 gggcggtcgt gcggctctat gccggcggca gggttcagct gcgcgaggtg cgcgcgggtt
    17461 cgtcgctcat gtcgcaatcg tcgcacgacc tcctcttcgg cctcggagcg gccgggaagg
    17521 cggagcgcat cgagatccgc tggccggacg gccgccgcga cacgctcacg gacgtgcccg
    17581 cgggcagcct cgtgaccctg gcagagggaa gagaagagaa gagggaattt ctcagaaggt
    17641 gaagagggcg gcggggagcg tccggggcgc gcgatccatc gatgcgcgcc gcgggcgccc
    17701 gcgcaccagc cataatcacc ttcgatgaag gacctcttcg aacgccgcac cgcgcagagt
    17761 gcgctgctcg tgtgctgcgc cctgactgcc agttctcttt cattcgcaga aactccccct
    17821 cttcgctttt cctccgagca ggcgtcgaag atcctgtcgc tcaggaacgt cggcctcgcc
    17881 cagctcgagg aggacaaggc gaaggacgcg cgggcgacgt tcgcgaagct cgctgagctc
    17941 gtgccggggg aggcgctgcc gctcgcgaac ggggccgtcg cggcgcttcg cgaaaaggac
    18001 ctcgcgggcg ccgaggccct cctcgcgaag gccggggacc gtgcggacgt gtgggcgatc
    18061 cgggccgcac tcgagaacga gcgcaaccgc cccgctgcga tgcgctccgc gctcgagaag
    18121 gcggccgccc tcgacgcgcg cgacctcgag tcccgctggc gctatgcgcg cagcgtcgac
    18181 tcggatccgg ccgccggcga cgccgagaaa gtgaagcggc gcgtctacct ctcggagatc
    18241 gcccagcgct cgccggcgaa cctgccggct cggctcaagc tcctcctcct cgaggtcgag
    18301 gccggcgacg cggcggccgc gaagaagacg ctcggcgagc tcgagccgct cgtgtcggac
    18361 ggcgacgcgc gcgcccgcca gttcctctcc gaggccaggg acgccctcgg caggggcgac
    18421 ctcaagacgg cgggcgtcaa ggtgcgcgtc ctcgagaacg tccagcgcgt gacgccgcgc
    18481 taccagcagt cgctcggcga ggtattcacg caggtcgtcg ggctgcccgt cgagagattt
    18541 tcttcgaagg tcgaggaggg cttgcgcgcg tcggccgggc catcgatccc ggtgtcgctt
    18601 cgggctccgg ttgcgtccga agagaacctc gagagacttc ttagaaaggt ggatctggcg
    18661 aactcgggtt cgcccggggc gtacacggtt ccggcgccct tcacggccgc cgcgttcttc
    18721 gactacgacc tcgacggcga cctcgacgtc tatctcttcg gcgcgtcgaa gtccgaccag
    18781 ctcctgcgca acaacctcga cgggacgttc accgacgtga ccgcaaagac gggcgacgcg
    18841 gcgatccagt cgaagaaggc cgtcgtctcc gacgtggacc gtgacgggga cctcgacctc
    18901 gtgacgatcg acgtgaaggg cgagctggtc ctccgggtga acctccgcca gggcgtcttc
    18961 cggacggtcc cgctccgcgt cttcggcgcg acggaccttg ccgtcgaaga cgtgaatggc
    19021 gatgggattc cggacattct ggtcggtacg gccaaagggc ttcgccttct catcggcaag
    19081 ggtgatggga gcttcctgga aggcgcggac aacggcctcg gcgggggcgc ggccccggcc
    19141 gcgatgcggg cggtggcgct gggagatttc gacaacgatg gcttcccgga cttgatcgcg
    19201 gcctcttcgg acggcttgcg agcctggcgg aaccagggag gcgggaagtt cgccgaatgg
    19261 gcggtcttct cttcaccttc aaagaaatct tctcttccga tctctccgga catcacgaac
    19321 gcagacagcc ttctcgccta cgacggggac agggacggcg acctcgatct cctcgtctcg
    19381 gcgggaggga agtcgtccgt cctcgtcaac gacggcggaa acgcgaacgg ctggctcacg
    19441 gtggtcctcg agggcctcgc gacgggctcg ggcaaggtga accgcggcgg cgtcgggagc
    19501 ctcgtcgagg tcaaggcggg aaacctcgtc gtgacgcgcc ccgcaggcct gtggccggtc
    19561 cacgtcggcc tcggtgtgcg cacgaaggcc gacgtggtcc gggcgacgtg gacgaacggc
    19621 gtcccgcaga atctcttcga ccagaaggcg aagacggtcg tgaaggaagt ccagcagctg
    19681 aagggctcgt gcccgttcgt gtacgcgctc gacgggcgga ccggcgtctg gagcttcgtg
    19741 tccgacgcgc tcgggcgcgc gcccatcggc ctcctctacg acggcatcca tctcgcgggc
    19801 gccgacacgc gggagtggct gctcgtgccg gaggggatgc tcgcgccgac gccggacggc
    19861 aggctcctcc tcgactacac ggaggagctc tgggaagcgg cgtacctcga cgaggtgtcg
    19921 ttgatggccg tggaccaccc gcggggcacg tccgtcgtgc cgaacgagcg gatgattccc
    19981 gggacgaccg agaaaaagct gttcacggtc gtggcgccgc ggcccgtgcg gtccgcgtgg
    20041 tcggacgcgt ccggccgcag tgaagacgtg acggagcgcc tcgcgcgggc cgacaaggtc
    20101 tacgtcgatc cgggtcccga gacgacctac cagggcgtac gcagggagca cgagctcgtc
    20161 ctcgacctcg gcccggtcgc ggcgggaagc cgcgtcgtcc tcttcctgag cggctggatc
    20221 ttctacacgg acacgtcgat caacgtgtcg ctctcgcagc ggaaggacat ggtccacttt
    20281 ccgcccgtcc tcgaggtgcc ggacggcaag ggcggctgga aggtcgcgat ggagtcgttc
    20341 ggattcccgg ccgggaagac gaagacgatg ccggtcgacc tgacgggcct catcgatccc
    20401 tccgacccgc gcgtgcggat ccgcacgacg atggcgatct tctgggacga ggcgtacgtc
    20461 acggtcgacg atccggccgt tgccgtcgtc accacggcgc tgcctcctgc gaaggcgacg
    20521 ctctcggagc gcggcttctc gcggcgtttc cgcgagacgc cggacggacc cgaggtcttc
    20581 gaccacggcg acacgagcgc cgccccggcc tgggaagacg tgcccggcag gatcacgcgc
    20641 ctcggcgacg tcacggagct tctctcgaag acggacgacc ggtgggtcgt cttcaaggga
    20701 ggcgacgcga tccgcatcga gtacgacgcc acgaagctgc cgccgctgcc ggcgggttgg
    20761 aagcgggact tcgtcgtcgt ctccgacggc tgggacaagg acttcgacaa gaacacggtc
    20821 accggccaga gctacggccc gtggccgttc cacgcgatgt ccgcgtaccc gtaccccgac
    20881 gcggagcgtc accccgaccc gaagttcctc gaggaaacgc tcacgcggga agtcggacct
    20941 gaacgattcc gccgagggct tggggaacgg tgaaggaaga tttgcttaga aaggtgaatg
    21001 cccgaggcgc gcccgcggcc gcggccgttc tcgccgcgct tctgatcgcc ccgccgctct
    21061 ctctttcatt cgcagaagaa tcttcctcgt cctcttcctc ttcctcctcg tctcttcctc
    21121 gtcaccccat ccgtttcacc gacgtcacga agaagtcggg cgtcgccttc gcctacgaca
    21181 ccgacctccg gcgcgggcgg aatctcgcca cgatgggcgc gggcgtggcg atgggggact
    21241 tcaacggcga ctcgtttccg gacctcttct tcacgggctc ggcctcgaac gggaagaagc
    21301 ccgacgccgg cccgtgcggc gcgctctacc ggaaccgcgg agacggcacg ttcgaggacg
    21361 tcacggcgcg ctcgggaatc aggtcctgcg gctggaggat gggcgcgtac tgggtggacc
    21421 tcgacgggga agggcggctc gacctcgtcg tgacgggtgt cggcagggtc gagatctgga
    21481 agaacctcgg gaacgggacc ttccgcgacg tggccgccga acgcggcctc gtcggccccg
    21541 gctattcggt cggcctcgcg gccggggacg tgaacggaga cgggagggtg gatctctacg
    21601 tcgtcggcta tctcgacacg gactacgcga aggagacctc gttcgggcag ttcgaggtcc
    21661 gcgtccccga ggactacgag gggcaggaag cgaaactaaa cgtgcagggc gcggacggcc
    21721 ggttcggaga gcgtgcgaag gaggccggcg tcctcaacac gggcggccgc ggcctctcgg
    21781 ccgtgttctt cgactacgac ggcgacggga ggcccgacct ctacgtcgcg aacgaccgga
    21841 cgacgaacgt tctctatcga aaccgcggcg acggcacgtt cgaggacgtg acggtcgagg
    21901 cgggggccgg caggcgcgac cacaaggagg cgcgcgccgg catgggcgtc gcgatcgggg
    21961 acctcgacgg caagggccgg ccggacattt tcgtcacgaa cttcgcaggc gagccgaaga
    22021 cgctctaccg caacgtcgag ggcgcgctgt tcgacgacgc gaccgaggcc tcgggcgtgg
    22081 agcgtccgtc gcttccgtac gtccagtggg ggacggacat cgtcgacctc gacgacgacg
    22141 ggcgccccga cctcgtcatg gtctcgggtc acctgatccc gaagatgatc atgtcgctgg
    22201 cgcggctgtt cgggaacagc aagagcctcg gcccgtacac gcacggcgac cgcagctaca
    22261 agcaggcgcc cgtcctctac cgcaacgcgg gcggcggccg gatggaggac gcgacgccgt
    22321 cctcgggcga tctcggcctc cttcgcctct cggcgcgcgg cctcgcggcg ggagacgtcg
    22381 acggcgacgg gcgcgtggac ctcgtgatcg cggcgatcag cgggggcacg cacgtcctgc
    22441 ggaacgcgac ggcggcgccg gggaacgccc tcgacgtgct gccggtggcg ggcggggaca
    22501 ggcgcacggt gctcgggacg aagatcgtcg tgaccgccgg aggggtgcga caggtgcagg
    22561 agttcatcct gcggccctcg tacgcctcgg gagcgtggct cccgctccac ttcgggctcg
    22621 gcgctgcgga gacggcgacg gtcgaggtga tcccgcccgg ggcgaccgag cccgcggcga
    22681 agttcgagaa cgtcgccgcg ggccgcctct acgtcctgag ggacggcgcg ctcacggaac
    22741 gacgcgcctt tcggcgttga gcgccccgcg cgtcttcgcc ttccacaagc cccggggcgt
    22801 cgtgtgctcc acggttgcgg agggaggggc gaggcccgtc acggaccttc tgccgccgga
    22861 gttccgcgcc tggttcgcag tggggaggct cgacaaggaa tccgagggcc tgttgctgtt
    22921 ctccgacgac gcggtcctgg cgcagcgcct catggatccg ggcggcgttg caaaggtcta
    22981 cgaggtcgtc gtggagggct tcccggacga cgaggcgctt gctccgatgc gtgcaggcgg
    23041 gaccgagttc gacgggcgca cgctccggcc cgtcgaggtg acgcgcgtgg gcaaagcccc
    23101 gcgcgggggc acgcgcttcc gcgtggtcct gcacgaaggc gtgaaccgcg agatccgaag
    23161 gctcttccgc gcggccgggc acaaggtgcg ccgcctgagg cgcgtcgcgg tcgggcccgt
    23221 ggcgctcggg gagatgagac cgggggaggg acgagagatt tcttcgagtg aaagagagcg
    23281 gctgcaggcg gctctcgcgg cggcgcgctg aaacccggag gcctcctccc cgtattcacc
    23341 ttcaaagaaa tccaacggtg aaaacaggga ggcatcacat gaaacgcacc acccgcgtcc
    23401 ttctcgctct cgtccccgcc ctgctcgtat tcgtcccacc cgccgcggcg cagatgcaga
    23461 tcaagacgcc cgacgcgagc ccggccgcga ccgtgagcca ggccatcggc atggccgatt
    23521 tcaccgtctc gtaccaccgc cccgccgtga acaagcggga ggtctggggc aagctcgtcc
    23581 cgtacggcca ggtgtggcgc gcgggcgcga acgagaacac gacgctcacg gccacggcgc
    23641 ccttcacgtt cggcggaaag gccgtgccgg cgggcacgta cggcgtccac gtcctcccga
    23701 cggcggacga gtggacgttc atcctcagct cccagtcgaa agcgtggggg agcttcagct
    23761 acgaccagaa ggaggacgtc gtccgcgcgg cggtcaagcc ggcggccgga gacttcacgg
    23821 agcggctcgc ctggtcgttc gacgagccca cgtccgacgg ggcgacgctc acggtccgat
    23881 gggaaaaggt ccgcgcctcc gtcccgatcg ccatcgacgc gaaggccgtc gccctggcga
    23941 acatccggga ccagctccgc gggctgccgc gcttcggctg gcagggctgg aacggggccg
    24001 ccgcgtggtg cctccggaac aagaccaacc tcgacgaggc catgacgtgg gcggaccggt
    24061 ccgtctccat gacgccgacg taccagaacc tgcggaccaa agccggcctc ctcgaactca
    24121 agggagacac ggcgggcgct gccgaggcgc gcaagaaagc ccaggctctc gcgaccgagg
    24181 ccgacatcaa caaccagggg taccagctgc tcggcgaagg caagatcgac gacgcgatcg
    24241 cggtcttccg caagaacgcg gccgaccacc cctccagctg gaacgtgtgg gacagcctcg
    24301 ccgaggggct cgagaacaag ggcgacaagg ccggcgccgt cgagaactac acgaaggcgc
    24361 tgtcgatggc gccggaggac cagaagaaac ggatcacgga cacgctcgcc cgcctcaaat
    24421 aaaggcggcg tcgtcctcag cgagggcctg catcgccggt gtctcgtaca cgtggatccc
    24481 gggcggcgaa tcgcgcgcga cggccaccat ggccttcgcg acgtcgtccg cccggatcgg
    24541 ccggtagcgt gcggccggtc ccagcatcgc gaccgacacg aggcgcacaa gggcgattcc
    24601 ggcggcctcg ccggcgcgcc tttcgcgtcg gtgcccgatg aggaacgacg ggcgaaagat
    24661 ctgcacgccc ctgtacggca gcgtccggac catgtcctcc gcctcgccct tgcagcgcag
    24721 gtagaagttg ccggactgcg cgctcgcgcc gaccgagctg acgaggagga actgcgccgc
    24781 gcccgcccgg accgacacct ccgcgagccg tgcgacgtac ccgacgtcca ccttgtgaaa
    24841 agcctcgggc gagcccgcct tccggatcgt cgtcccgagg cagcagaaga cgtcctcggc
    24901 gcgcgggaac gcggacatcc gtcccatgcg ctcgtagtcg accgtgcgct gctcgagttt
    24961 cctgtgcgtg atgttgacgg ggccgcgcac gaacgcgatg acgcggtcgt gcgtcccgcc
    25021 ggcgagcagc cgccgcagga cgcgctcgcc gacgagcccg gaagctcccg ctacgatcgc
    25081 cgaacgcatg agttggacgc atcataagcg agaatccgtc tcgtgcagac cgtcgagacg
    25141 atcctgacgc ttctggggct gacgacgctc ctcgcgttcc tcgcccagaa agcaaaggtt
    25201 ccgtacccga tcttcctcgt gatcggcggg atggggctcg cgttcgtgcc cgggctgccg
    25261 gccgtctctc tcgacccgca gctcgtgttc ctgttcttcc tcccgcccat cctctacgcg
    25321 gccggctact tcacgtcgtg gcgcgacttc cgcgccaacc tcttctccat ctcgctcctc
    25381 gccgtggggc tcgtcgtggc gacgaccgcg gccgtcgcgg cggtcgtcgt cgcgctcgtc
    25441 ccgggcgtgc cgtggcccgc agcgctggtc ctcggcgcca tcgtctcgcc tccggacgcg
    25501 gtcgcggcga ccgccatcgc ccagcgtgtc cgcctcccgc gccagatcgt cacgatcctc
    25561 gaaggcgaaa gcctcgtgaa cgacgcgacg ggcctggtgc tgctgaagtt cgcgagcgcg
    25621 gcgatcgtga cggggcacct cgcagtcctg ccgggcctcg cggagttctg cgtgatggtc
    25681 gtcggggggg tcgcggtcgg cctcgcgttc ggctggctgt tcgcccagac ccagcgctac
    25741 ctcgacgaca cgatcctcgc gacgacggcc tcgctcctcg tctcgttcgt gacgtacatc
    25801 gcggccgagc acgtccacgt ctccggcgtc ctcgcgacgg tggcggcggg ggtcgtccag
    25861 gggagggtcg tgccgcggat cagctcgtcg aagatgcgca tcgagagcac gagcgtctgg
    25921 cagacgttca tcttccttct caacgcgctc gtcttcgtcc tgatcggcct ccagcttccg
    25981 gctgtcctgg acacgctgga ctggccctgg ccggtcgtcg tggacggcat cttcgcgacc
    26041 ttcggaacga tggtcgtcgt ccgcctcgcc tggatgttcc cgggcgcgta cctgccgcgc
    26101 tatttcttcc cgaagttccg cgccgctcat ccctatccgc cgtggcaggc ggtcgtgatc
    26161 gtgggctggt cggggatgcg cggtgtcgtg tcgctcgcgg cggccctttc actcccgctc
    26221 gtcctcgcgg acgggcggcc gttcccggcg cgcagcctga tcatcttcat cacgttcgcc
    26281 gtcatcctcg gcaccctcgt cttccaggga ctcacgctcg caccgctcgt gcgctggctg
    26341 cgcgtcgagg aggaccgctc gggagagaag gaagagcggc gcgcgcgcct cgcgctcgcc
    26401 gaggcggctc tcgaccacct cgacggggtg atggacgagc ggaacctgca ggcgcaccag
    26461 gtgcacggcg tgcggcagga atacgctcag cgcgtgaggg cgatgaaggg cctcgagggc
    26521 gaggggcccg cgcacgctcc gctcgagctc tcgaaggaac tgcggcgcac cgcgatcgaa
    26581 gcccagcgca aacagctcct caagctgcgc gacgaggagg tcatcggcga cgacgtcctc
    26641 caccgcctcc agcacgagct ggacctcgag gacgtgcgga tgcggtgagc gcgcctagcg
    26701 cttcgcgggc tccggcgcgc cgccctcgac ccacttgtag agcccgggcg cgaggtcctt
    26761 cgcgctcgcg gtcgtgccgt ccggccagcg gacgtccacc gcgtcgagtt tcgcggccgc
    26821 gccgagcccg atcgtgatcg tccgctcgct gacggacagg tagccgtcgc cgcccgaaac
    26881 ctcgtcgata cgcttctttc cgcccatcgt gcacgtcacg cgggcgccga cggcgcctcg
    26941 attcctgccg ttcgtcgcga gaacgaggcg cacggccttc cccgcgccct ccgagacgtt
    27001 ccggtagacg tgggcggggc cgccgttctc ggtgatcacg acgtcgggct tcccgtcgcc
    27061 gtcgaggtcc gcgaccgcga ggccgcggcc gaccatcggc acggcgaagg cggggcccgc
    27121 ctgcgacgtg acgtccgcga atcggcccgc gccggtcccg cggaagagga gcggcgcctg
    27181 gcggtacgtg acggtcggct ggacgtcctg gacggacgtc tcgacgtggc cgttcacgcc
    27241 cacgaggtcg aggacgccgt cgaggtcgaa gtccgcgaac gcgacgccga aggtcagcgg
    27301 caggagcgac gtgcgcccga tgccgctctg gacggactcg tccacgaaga agtcgccctt
    27361 cggtccggcg ctccacacgg accacatctc gttcgagaag ttgccgacga cgagcgtcac
    27421 gccgccgtcg ttcttcgtgt cgccgaacgc gacgcccatg gcgccgcgcg cgcggccgtc
    27481 ctcgccgacg gcgacgccgg cctcgatcgc catgtccttg aagtgcggga tgccgttcac
    27541 cgtctccacg ttccggcaga ggttgttcgg cgaggtgtcg ttcgcgacga cgaggtcggg
    27601 ccggccgtcg ccgtcgaagt ccctcacggc gaccccgagg gccttcccga gcgggttcac
    27661 gaggcccgct tccttcgtga tctcccggaa cttcccggcg ccaaggccct tgtagagccg
    27721 ggacgagacg ccgttgtagc gctccggcgt gcagtaggac ttcgtcttgc cgtccagcga
    27781 gcagtgcatg tccgtcttcg gcgtccagtt cacgtactgg ccgacgaaga gatcgaggac
    27841 gccgtcgccg tcgaagtccg cgaacgccgc ggacgtgccc cagcccgggc ccgcgagccc
    27901 ggaacccttc gtcgcatcgc ggaaccggcc cttgccgtcg ttgacgagca ggcggatgtc
    27961 gtcgaggccc gtgatcagga gatcgggcgt gccgtcgtcg ttcacgtcgc ccaccgcgac
    28021 gcccatgccg tagagcgaga tcgcgaggcc ggcatccttc gtccggtcct cgaacttcat
    28081 cgcgctcccg ggctccgtga cgtttttcca gaaggaaagc gtgggctgct gcgcggccct
    28141 cgggtcgccc ggccaccagc gcccgttcac gaacaggagg tccacgcggc cgtcgccgtc
    28201 cgcgtcgaac gccgcgacgc cggagcccat cgtttcgggc atccacttct tgccggcggc
    28261 tccgttcacg tgaacgaacg aaacaccgga ggccttcgtc acgtcctcga accggatgga
    28321 cgacgccacc ttcgtgggct cgggcgccgg cgtcggggag acgggcttcg gggcgggctt
    28381 gccgcacgcg gcgagagcaa gagtcaggag aacgggaaaa tgtctttgaa tggtcatggg
    28441 atcgaaatgc tatcgcatgc ggcggcctca cctttcatcg taaagtcgca cacatgcgaa
    28501 ccgcccgccc tcttcctttc aaagaaattc ttctcctttc cctttccttc gtgttcgcca
    28561 cggccgcggc cgccggcgac gctcccgcgc ctcccaagct gcgcctcccc gccggcgtcg
    28621 cgccggtgcg gtacgccgcg gagctgtggc tggacccggc gaaggagacg ttcagaggga
    28681 agatggagat tcggatttct ttgaaagggg aatccgacac gctgtggctg aacgggaccg
    28741 agctgacgat cgagtcggcc tcggccgtgg cggcgggtgg gaaagacgcc atcgcggcgg
    28801 agggatcggc cgtgggcgcg gactacgtcc gtattcactt tcaaagaaat cttccgccgg
    28861 gcgaatacga cctcaacctg gcctatacgg gccgcgtcga gggcaaggac acccagggca
    28921 tcttccgcca gaaggacggc gccgactggt acgccttcag ccacttcgag gcgatctacg
    28981 cccgccgcgc gttcccgtgc ttcgacgagc cgtcgttcaa gacgccatgg cagctgacga
    29041 tccacgcccc gaagggcgcg atcgccgtgg cgaacacgcc ggtcgtcgcc gagcggtcgg
    29101 acggggacgg agcgcgggcg ttcgtcttcg cgccgacgaa gccgctgccg agctacctcg
    29161 tcgcgttcgg cgtcggcccg ttcgacgtcg tccccgcggg gacggccggg cgcggcaaga
    29221 ccgcaatccg catgatcgtc ccgaagggcc gcgccgcgga cgcgcgctgg gcggtcgagt
    29281 cgacggggcc catcctggag gtcctcgaga actacttcgg catcccgtac ccgtacgaga
    29341 aactcgacca cctcgtgatc ccgcagaccg tgggtttcgg cgccatggag aacgcggggc
    29401 tcgtgacgta ctcctcgagc cttctcctcg cgaagccgtc ggacgagacg atccgcttcc
    29461 ggcgcgcgta cgcgagcgtc tgcgcccacg agacggccca ccagtggttc ggcgactacg
    29521 tcacgacggc gtggtgggac gacatctggc tgaacgaggc gttcgcgacg tggatggctt
    29581 ccaagatcgt ggacacatgg aagcccgagt ggagctgggc ggtgcagcgc gcgtcgtccc
    29641 ggacgggcgc gatggaccag gattcgctcg tgacggcgcg gcgcatccgg cagccgatcg
    29701 cggggaacga cgacatcgtg agcgcgttcg acggcatcac gtaccagaag ggcgcggccg
    29761 tgatcgccat gttcgagggc tgggtcggcg aagagggctt ccggaagggc atccagcact
    29821 acctgaagac ccacgcgtgg ggcgcggcga cctccgacga cttcgtcgcc gcgatcgccg
    29881 aggcgacgaa gaaccccgcc gtcgtcccgg ctttccggtc gttcctcgac cagccgggcg
    29941 tcccgctcgt gacggccgag ctcgtgtgcg ccggcggcgc gccgaggctg atcctctcgc
    30001 agaagcgctt tctcccgacg ggctcgacgg ggtcgaaaga ggagacgtgg cgggtgccgg
    30061 tctgcgcacg cacgggcgac ccgggtgcgc cgacgacctg cacgctcctg acggagccgg
    30121 cggggacgct cgcgctgccg ggcgcgtgcc cgtcgcgcgt tctcgcgaac gccggcagcg
    30181 ggtattaccg cgtcctctac aaggggagcc tgctcgggaa ggtcctcacg gacggcggca
    30241 agcatctgac cgcttcggag cgcatcgcga cactatccga cgtcgccgcc ctcgcgcgca
    30301 gcggcgacgt gccgatggcg acggcgctcg cgctcgtgcc cgcgtttgcc agcgaccccg
    30361 accggcccgt cgtcgaatcc gtccagagga tcgccgcggc gccgcgcgac ctcctcgtca
    30421 ccgacgggat gcgtccgcgc taccgccgtt tcgtgagcga cgtgttcggc gcgcgcgcga
    30481 agtcgctcgg ctggaccccc cggaagggcg aggacgagga cacgcagctc ctgcgcgcct
    30541 cgctcgtccc gtttgttgcg aacgagggcg acgagccggc gctcgtggcc gaggcgggct
    30601 tgctcgcgaa ggcgtggctc aaggaccgct cggccgtgga ttcgctcctc gcgtcggaag
    30661 tgctcgacgt ggcggcccgc cacggcgacg tcgagctctt ccaggcgtac ctcgccgagg
    30721 cgaagaaggc gcaggagcgg cgtgagcgtg cgcggctgct gggtgcgctc ggattgttcc
    30781 gcgatccctc cgtcgtgccg gcggcgctga agctcacgct ttcgcccgac ttcgacgcgc
    30841 gggagacgat cggcatcctg cgcgaggcgg cctcgagccg cgagacgcgg ccggaagcgt
    30901 gggcgttcct caaggcgaac ttcgaccgga tcgcagcgcg gctgccgcgt gagtcgcccg
    30961 cgcgcttccc gatgctcgcg gccgggttca gcgatgcgga gaagcgcgcg gacgtcgagg
    31021 cgttcttcaa ggacaaggcc ccgaagtaca tgggcggccc gcgctacctc gcccaatctc
    31081 tcgagcagat ccagctgcgc ggcgcgttca aggcggccca gcaggagagc gtcgacggct
    31141 tcctgatgca ctacgagccg cggccgacga tcgacctcaa gccgcagtcc gggatgtagt
    31201 gtgccgacgg gaacccgccg gcgagcgccg acgtgggaga gcgaggcgct cggcgtcctc
    31261 gcgggcgagt tcccgttctc gaaccgggcc gtgaccgagg cgaagatccg cggaaggctg
    31321 aagcggaaga ggctcggggg ctacgacgcc gcgcgcgtgg ccgtcctgcg gcgattcaag
    31381 gacgcccttc aaagggagat cgcgaagggc gaggcgtcgc gcttctatca ggggccccac
    31441 gggcgctacg tggacgtccg cgacttcgac gtggcgcgcc tgaggaagga aatgtcccgc
    31501 cgccatccgc gcgtccctaa ggaagaggtt tcctggttcg ttccattcgc ggttttcctg
    31561 taccacgttc tttgagggca cgcggtcatc tcacgaccgt tgcgggggat tcgacatgaa
    31621 ggcatccgtg ttcgtcggca cgagcgtcga cggctttctc gcccgcctgg acgggagctt
    31681 cgacttcctg ggggacggcg gggagccgca cggctacgag gagttcatgg cgacggtcga
    31741 cgcgctcgtc atcggccgca acacgttcga cgtcgtccag aagttcccgg actggccgta
    31801 cggaacgaag cccgtcttcg tcctcagccg gcgcgccctt ccgcccgtcc cgcccggggc
    31861 cgtcgtggag agaatggcgg gtgagccggt cgagatcgcg gccctgctct ccgcccgcgg
    31921 gatctctcac gcatacgtgg acggtggaat caccgtccag cgcttcctga gggccgggct
    31981 cgtccagcgc ctcgtcgtca cgcgcgtgcc cgtcctcatc ggtaccggaa tcccgctctt
    32041 cggcgcggtc ccgcgcgaca ttctcctgaa gcacgtcgcg acgcgttcgt atccgagcgg
    32101 cctcgtccag agcgaatacg aggtcgtggt ctgagccggc gctttcggac tcgccctcag
    32161 gcggccgagc cgagggtctt ccagtagcgg accaggccgg tgacgttcat cgcgaagggg
    32221 agcgccgtct tgtaggcctc caggcccccg gcgccaacgc ctgtcgcgag gagccgatcc
    32281 gtgagccacg cctcgaagat cggaacgatg gcgggctcgt ccttgccctc gtccaggcgg
    32341 gcgcggatga agcccgccca ggcgtgaagc tcttccatca tctcgtcgag gcgccgggcg
    32401 acatcgccgt ggcgcccgaa gtgcgtcggg tagaaggcgt cgggccgcgc ggccttgatc
    32461 cggtcgatcg agcgcgccca cgtcggcagg tcgatgtcgg gggggggagt cgggggcagg
    32521 ttcgggccgt cgccgatcgc gacgccgccc acgtcgcccg tgaagacgtt tccgtcgagg
    32581 aggtaagcgt tgtggtggat ggcgtgcccc ggcgtctcga gcacgcggat ccgggtatcg
    32641 ccgaccggca cgtcgagccc gtcggacgtg accttgatgc gctccgcggg gatcgcctcg
    32701 agcgttcccc acaacgtgtc catctgctcc ttgtagatcc gcttcgcgga ggcgaggagc
    32761 ttcgaggggt cggccatgtg cggcgcgccg cgcggatgga cccagacgtt cgcgccggcc
    32821 cgggcgaggc gccaggccgc gcccgcgtgg tcgaggtgga tgtgcgtgac gaggacgtcc
    32881 ttgacgtcct cgggcctcgc accgtgcctc gcgagggcgg cgacgaggtg cggccacgtg
    32941 gagtcggggc cggtctcgat gagggcgagg cggccgccgg actcgacgag ataggcggcg
    33001 atcgtctcgc cacggaagaa ctcgaggtcg agggtgtgga tcttcatcag ggcgcgatcg
    33061 cggccgccac gggcacgccc ggccccttct ttctctctcc cgcctgcttc gccggcggct
    33121 ccgggcgcgc gaacgggcgc ggcgcaccct gcggccggtc gtccttgagg accatctcgc
    33181 ccttcggcgc atagcccttg tacgcgtagc ccttcggccc gatctcggcc agccaggtgg
    33241 gcggctgagc gggcggcggt acggcgtcgt cgtgcacgtg gaccggaagc gattcgcggt
    33301 tgtcgagcgg gtgcgcgatg cggtattccg cgagcagagc gcgggcggac tcgtcgtcct
    33361 tgaagaagcg gtaccacttc tcctcggcgg cggcctcctc cgtgcgcccg agggccttga
    33421 ggcagagcat cgcgttgtaa tgcgcggcga gatcctcgcc gtcgatcgcg aggaccgcgt
    33481 cgatcggcgg gagcgcgtcg gtgtagcggc ccgcgaggta gagaacgcgc gccttctggt
    33541 tcaggacgac gcggtccttc gggaacttcg cgaggacgcg gtcgaggtcg gcgagtgcgt
    33601 cgtcgagccg gccctcctcc ttggagacga gcgagcggaa gtactgggtc ttgccccagc
    33661 ccggccgccg cttctcggcc tcggcgagcg cggccttcgc cgcggggagg tcgccctcct
    33721 gcaccttgca ccgcgcgagg ttcagggggc cgtccggctt gtccccggcg agctcggcga
    33781 ctttcttgaa cgcggccgag gcgcccttga ggtcgccctg caggaggagg ccgatcccgt
    33841 agtcgttcca gcggatccac tgtttctcgg gccagtccgg gtttccgcgc ttgacgtcgt
    33901 tcgcgagcgt ggccgcgggc gcgggcgcga ccggcagcgt gacggagtcg gccgaaatgc
    33961 gcgtgacggg gagctccggt gcgttcgggc cgcccacgaa gatggagtag tcgcgcgaga
    34021 acttccggta gttgagcgcg gccgcgagcg tgagggagcc tgccgcgtcc ttcggcaccg
    34081 tgaagcggta gtggacggtg cgggccgcgc ccggcgggat gaagttgttg tagaggggga
    34141 cgtggatgtc ctgcggctgg cggcggtcca tgaaggagcc gtcgtgcgca atcgcgagct
    34201 gcgcgatctt gtcggccgcg ggatcgaggc ggccgttcgc gtcgagcgtg cccgacgcca
    34261 tcacggagcg cggcccgttg gcggccttga actccaccca cgtctcgttg ctgtcggacg
    34321 tgccgttcgt gtacgggtgt ccgagcgtgc gcgtgcgcac gacgacctcg acgtccacgg
    34381 tctcgccggg atggagagac ggcagcgaag gaccaagagg gatgatctcc gagcctcgtt
    34441 tcagggcgaa gacgtcgatc gaaagtgatc cgaccaatac cttcttacga atgtcttcga
    34501 tggtctcctt gtcccctctc agggccggca gcgccgtgtt ggccgccggg aagaggtggt
    34561 cgtggagcat gccggccacg tttccgaact ccttggaggg catgagcggg agatggcagt
    34621 cgcggcacgc cttcgtcttc ggggggtcgt agaacgaccg gaccgcgttg ccggacacgc
    34681 ccgagtcgta ccagctgtcg tagtggttct gcccgcgcat ccagcggtag tcgttcagcg
    34741 gcgggaggag cccgaccttg tggcacgtcg agcagaactc cggcgtccgc atgaagggct
    34801 tcatgaacgt ctgccggtgg agggacggct ccatgcggat caggagcttg ttgacctcct
    34861 tgagcatctt gttgtccgag aacgcgaacg ggtactgctt gctctcctcg atgacgtagc
    34921 caccgttgcc ccgcaggtcc ttgacctcgg cgatcgaatg gcaggacatg cacgtgaggc
    34981 cctgctgccc ctcgaactgg tcgtacgaga acgtggccat cgtcgccttg cccatctggc
    35041 ccgtgaagag gacgacgggg tcgtggcagc cggagcacca cttcgtcttc tcgcggccga
    35101 cgcggtcggc catgagctcg acggagcggc ggtagaacgg gttgttgaag gacgagaagc
    35161 ggtgcgcgga cttctcccag cgcttgaagg aatccgggtg gcagtcctgg cagtaggcgt
    35221 tcgcggagag cctcttcccg tcgagaaggc cctggtcgaa cgtctcggcg gacgagagga
    35281 agaactgggt gtcgccgttc ttgttcacga tgcgctgcgg cggcgtctcc atcttgtgga
    35341 gtcccgccat gacgagcaga aaggccgcga cggcgccgcc ccactgcgcg agcatcctga
    35401 agtgcagctt gtggaccgcg gcgcgccggt gaaggatgaa cgcgacgagc gcggccggga
    35461 tcgcgaagac gtgcgcgagg tagatcggcc ggttcgtgag cgtcgcgccc ttgagggcca
    35521 tcgtgacgcc ggtgccgatg cacaggaggg acaggcagac gatcgtgatg cccgcgatct
    35581 tcgcgttccg gttccggatg ttcgcgagca tccgcgcgcc gtgcgtgaac gcgaagacga
    35641 ggaacgggat cgcgatgagc aggcccagga cgaggtgcat gagcagcatc gccatgaaga
    35701 acgaggagta cttcgtgaag gcgacgaggt agatcgagtt cgccgccatc agcccgagcg
    35761 gcacgagcag gatcgtgtag agccgcttct gccacgggtg aaggacgccc ttggcgcgga
    35821 ccgcgccttc gttccgggtg ttcatgggcg ggaattctag cgctgagagt cgttctcggg
    35881 tcgccggcgg cgggggcggg cggcccacgc ccgccgcttc tcgcgctcga ggacggacgt
    35941 gagccggcgc tcgagagagc tgcgcaccgc gcccttgaga cccgcggtcg cgagagcctc
    36001 tcggagccgg gcctccgcgt cggcggcgtc gtacgcgccc tcccagagaa accggtccac
    36061 gaagccctcg agcgcccagc tgtaagttcc gtaggccggg ccgaggagga aggccgcgag
    36121 gcgcgggagc gggatgtcga ggttcagcac gtccagcatc tcgctgccga gagggatcgg
    36181 gtggtagcag tcgtccttga cgtcccagga caggtaccag cgccggccgc aggccgtgca
    36241 ccacgcgagc acgaggtccg cgcggcgccg aacctcgagg tcgggcccga atcgcgcatc
    36301 cgggtgttcc gagagcgggc gccacgtcgg ggggcgcacg ccgtcggccg tgctcgtgat
    36361 ccagtcgcgc gtgaaggcgt cgcagaggca gtcgtggctc gtcatttcga gtccagctcc
    36421 ccggccagcc actcgacggc gtcgcgaaac gcctcgttcg gaagaaggcc cttttcattc
    36481 ggcgaataga gcttgaggac catcccgtct ctcgcctcgg gcggttcggc cgagtggagc
    36541 gccgcgaaga gaagatgctt agaagggtaa gaggggaagg gaggtggccc gattaccgcc
    36601 gtggcggctc cctgagcgcc ggcccgtgtc caccttctaa gaaaatcttc gtctgggcga
    36661 acgagaagcc agccgatgcg ctccgcgtcc gcctccacac gggtccgaag caaatcgtcc
    36721 ttcggctctc tcccgacgag gacgacgaac ccccgatgcg ccgcctggtc gaggatcacg
    36781 tcctttctgc agtcgccctt cgccggacac gccacgacga gcgccggacg gcccttcgcg
    36841 ggcaagggga ccttcttgtc gggatagatg gagccgggga ggagtggcgc gtagagcttc
    36901 gggatgtaga gcttcccgcc cggcacgctc tgggtcttcg cccagtcgga cgacgcgcag
    36961 ccggtggcca cgagcgcggc gagcaatgcg aaggctgcgg gacgcgtcct caagattctt
    37021 cgaataatga acgctgccgg ggcgcggagc catcctgtcg cgcgccggcc tctcggtcgg
    37081 ttgtattcac cttcaaagaa atcttctctt ctctttcgtt cccctccatt cccagccaga
    37141 cgagctctcc ggtgtccatg aagaagagcc cggcgttcgc cggcttcttc gtgagggcct
    37201 cccatgcgcg gcggtaatgc gccacctggg gcgcgtagtg ggcgaccagg gcggcgagtc
    37261 cgtcgcccac gacgtcggac ttccagtcca cgatccgcca gacgccgtct tcctcgaaga
    37321 cgaggtccat cgcgcctttc aggagcgtct cggacggtcc ggcctccagg ccgaggtcct
    37381 tcgacgggac gaccatctcg aacgggacct cgacgtaccg ccgcggcgcc ttcagcgccc
    37441 gcgcccaaag gtcggatgcc gtgacggact ccgcgacgcg gagcacctcg ccgaggaggt
    37501 cgggcgcgac ctcctcctcg cggaggagat tctgggcgag gggcgcgagc ggggcgccgg
    37561 gggtccgcat ggcggcctcg agcagctggt gcaggacacg tccccacgcg gtgccgcgcg
    37621 cttcctcggc cggcgacggg gcgcgcggcc cctccttctt cgcgagcgcg gtgacggt
//
LOCUS       DAOMFY010000010        37110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_356_length_37110_cov_7.889273, whole genome shotgun sequence.
ACCESSION   DAOMFY010000010 DAOMFY010000000
VERSION     DAOMFY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 37110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37110
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_356_length_37110_cov_7.889273"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLB01_01885"
     CDS             <1..335
                     /locus_tag="PLB01_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76077.1"
                     /translation="APSARATPGGTPAPAALAGRLKILALPYARVLAVVDAAGKAVAL
                     PKDATTPLLLRDLAPGTYRVALAGPDGSRRVERRVDVAAGAAELLSEPFETPSSLAQL
                     LGTGGSKP"
     gene            332..1777
                     /locus_tag="PLB01_01890"
     CDS             332..1777
                     /locus_tag="PLB01_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76078.1"
                     /translation="MTKPFFRGIPALAVLLAAWAPPAAAQLDSALLSKLRFNLTNPGG
                     KSLAMGGAFSAIADDATAALANPAGLGLISSIEIAVSGKRVNETLALSTARSVATGSL
                     VTPYPPVTTSSVGLDASSTGVEFAGVVVPISRRLVAALTYAENLRFEGDAGPGGYQYL
                     ELRDNRSGGTTRRDFLFGYREYGSAKLSNRLLAASLAYRVTESLRLGGGVTLNRSTFD
                     LEGDAGGPHRIVSQTYLSPTAQETRTVTLGVEGLSGTQVGFVAGVHADLLSDGRLTFG
                     ADYRWTPAADAALVIAGDVPPALASQTSRAFKFRVPSDASVGLAAHPMPGLTIAAEVQ
                     WVRYGDIFDRALPVVSYAGLAGPFPGVPVTGVLADLSPAPNVFVPRLGFEYVAGGKDV
                     RMALRAGWHREPAHGVTANLAARDGSGQPYPITDPPYSESVAAVFDGGKADDRFTGGL
                     GLTVSRALSVDFGFDVGRDSQQFAVSLFYRF"
     gene            complement(1774..2916)
                     /locus_tag="PLB01_01895"
     CDS             complement(1774..2916)
                     /locus_tag="PLB01_01895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQQ76079.1"
                     /translation="MLFTLPQVLVLFCALLAASLGVLAVLLLRWNRARVELGLYAVLA
                     FAVAFWLATPLLTPVPVPISREILGRLVRALAFLLPAIGFLFLLRILGLGARGLRLAI
                     LAPAVLGTALALALPASAGWLDSLAVASLLIVGAGVAWVLARKENRRRDTFLAFAATG
                     ILLAGGVAEIASRRLPEPSSASGVLLGTAFLAFTALLLPTAADEERRLFTRATTDALT
                     NLPNRGTFLARSRDELFRAERTRRPLALVLLELDGFAAIRKEKDSAAVDEIVAAYGQG
                     IARAIRGIDVPARWGPDSFAALLVEAEESSTRAAVDRIRAFAAKAAGVTASAGIATRR
                     PLEKTTIEDLIRRAEAALDDATRDGARDASAMETRDEPGAPGTAGA"
     gene            complement(2955..3884)
                     /locus_tag="PLB01_01900"
     CDS             complement(2955..3884)
                     /locus_tag="PLB01_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ76080.1"
                     /translation="MRVVVTGAAGFLGSHLTDRLLAEGHDVVGLDNLVTGDVRNIAHL
                     AGNRKFRFVFHDVTEYIYLDGPVDAILHFASPASPIDYLQIPIQTLKVGALGTHKALG
                     LARAKGARFLLASTSEVYGDPLVHPQPETYWGNVNPVGPRGCYDEAKRFAEAMTIAYR
                     NFHDVDTRIVRIFNTYGPRMRPNDGRIVPSLIGQALRREPLTVFGDGSQTRSFCYVSD
                     LIEGIHRLLTSGYRDPMNIGNPAEMTVLQFAETIKRLTGTPAPIVFRPLPVDDPRQRR
                     PDISLAKATLGWEPKVALEEGLGSTIRDFEARA"
     gene            complement(3884..5179)
                     /locus_tag="PLB01_01905"
     CDS             complement(3884..5179)
                     /locus_tag="PLB01_01905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQQ76081.1"
                     /translation="MNICMVGTGYVGLVTGACLSDFGMDVSCIDKDESKIALLNKGVS
                     PIYEPGLEELIHKNEKAGRLRFSTNVAEAIERSLVIFIAVGTPPKDDGSPDLSFIFQV
                     AETIAEHMNGYKVVVTKSTVPTGTGAQIEEILKKNGKYKFSVVSNPEFLREGSAIEDF
                     MHPDRVVIGSRDEEAIAIVKDVYSPLAIAGVPFVVTDVESAELIKYASNGFLALKITF
                     INEIAMMCEKMGADVKDVARGMGLDKRIAPQFLNPGPGYGGSCFPKDSSAVVDLARKV
                     GYTFELMEATLSANDRVKARMIDKVEGLCGSVDGKRVALLGIAFKPETDDIRESASLK
                     LASDLLARGADVVAYDPAAMENARREMPGLKLAEDQYACIAGADVLVLATDWNQFRKL
                     DLPRIEKTMKAKNFVDLRNLYEPKEMKKAGWNYVGLGRA"
     gene            complement(5252..6151)
                     /locus_tag="PLB01_01910"
     CDS             complement(5252..6151)
                     /locus_tag="PLB01_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQQ76082.1"
                     /translation="MRALVFDNGRAAVRDVRRPSPRKGFALVRILLSGICNTDLELKR
                     GYHGFSGIPGHEFVGIVEGPAASPWLGKRVVGEINLACGLCSWCAQGLGRHCPTRTVL
                     GIRGHAGAHAEWLTLPEANLFEVPPGVADEEAVFVEPLAAACEILDQVAVPSGTRAAV
                     LGPGKLGRLAAAVLGTAGADVALLGRTTRAKAASFDLVVEATGSPAGMPRALALVKPR
                     GTIVWKSTHHAPARFDAAPLVVNEVTVVGSRCGRFEPALEFLRMKRADVLFLLSSEFP
                     LGQAVRALREAGRPGVLKVLLRP"
     gene            complement(6160..7188)
                     /locus_tag="PLB01_01915"
     CDS             complement(6160..7188)
                     /locus_tag="PLB01_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ76083.1"
                     /translation="MSRVLVAGGAGFLGAAIARQLLGAGEDVFLLDTFDDAGEGRALK
                     EERAASVRRHPRLTVVRGDGTDADLLEGVFAEHRPAAVVNAMRLPLDSAGLGPLVEAS
                     RQAGVGVVVHLSDAALYGPRDATGLRAREDEPIEPGEDRDLLAKAAEEEAFLASGLPF
                     VILRVFAAAGPGAGPSRFPIDALEALLAGEEVFLPDDAPRDFVHPQDVVRGTLFALRK
                     RPIGEIVNLGFGLAVKPSEVVRALAVRAALECRLTVQGDAARPPRIADMEKAWQTLGF
                     SPQLGLGEIVWDTVRARLFPAEAARAYQPAEAPAPQPPVEPPKPVSRRELFDMFRRPL
                     GGARKPGS"
     gene            complement(7185..8357)
                     /locus_tag="PLB01_01920"
     CDS             complement(7185..8357)
                     /locus_tag="PLB01_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQQ76084.1"
                     /translation="MVRADIRTKTRVGVILFASFILLAGALLFIGGTKGFFVRRTSYF
                     ARFPNSQGLLPGNQVRLAGVVVGAVRDIDVPRAPGQDLTVHFDIERRYQHLVKTDSRA
                     EIKTIGLLGDKYLEVTPGSPDKPDLDPDHEIIAFRGAELDKILAGSGDLVDNVVAISK
                     SLKNILGRTEKGEGFLGEITSETKNGAELSKSLRQTLDNLNGLVVDVRQGRGLVGRLL
                     HDEKAADDVLAELKGATASLNRILATVEKGTATGEGLVPALLSDPEGKKKFFAMVDSL
                     KTTADSLASFSKDLSGGSGLLPKLVKDEAFAKEFTDDLRKLSSHLANVAAKLDSSDGT
                     AGRLIADPSVADAINDILVGINESKTLRWLVRDRQKSGIQKRYEDAQKAPVPTPAP"
     gene            complement(8380..9183)
                     /locus_tag="PLB01_01925"
     CDS             complement(8380..9183)
                     /locus_tag="PLB01_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ76085.1"
                     /translation="MRPLSETGVMRILRTGMPAGAPIIEFKGVSKAYGQKKVLDEVDL
                     EVRRGEVLVILGGSGTGKSVSLRHMNGLEQPDAGEVWVDGEEIAHLPEESLVPVRKKV
                     GMLFQSGALFDSMTVFENIAFALREHTSWPEDRISERVAEVLGFVNLGRDVERLLPSS
                     LSGGMKKRVSLARTVALKPEVLLYDEPTTGLDPVTSMTINRLIKDLNTRLGTTSVVVT
                     HDIVSALFVADRITFLKNARFAFTGTPEEAKASDVPELREFLSAGQSLA"
     gene            complement(9180..10022)
                     /locus_tag="PLB01_01930"
     CDS             complement(9180..10022)
                     /locus_tag="PLB01_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ76086.1"
                     /translation="MAQPQYDSTTQYIAVLKEPLWKMARLRRWFGNVSFHLGGVAWLV
                     RDLVRASREKWETSEILRQMDALGVQSISITNLTALFTGMVLALQTASALSQFGGKLF
                     VGRVVVLSITRELGPSLTALMVAARVGAGITAELGTMAVTEQVDALRALGASPARKLV
                     LPRVLAVTLMLPILTLLADFIGMVGGLFIAVVDLKIGASFYLSTVSQSVHVNDILHGL
                     FKTPFFGFEIGIIGCYNGLNATGGADGVGRATTVAVVIAAIVVLMSDFFLTKLFLLLP
                     LGLK"
     gene            10067..10786
                     /locus_tag="PLB01_01935"
     CDS             10067..10786
                     /locus_tag="PLB01_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQQ76087.1"
                     /translation="MPAGLRDALVTTWGWANIAVSTALFGTLAILTSWVPPRGRLYLF
                     WARHWAKSLLWLNRIPARIEIAEEAAALPQAIYMSNHESGIDILLLLLAIRQDVRFLA
                     KRELFFVPFMGWSMWLAGFVPVDRRRTDKARDVLSSLDRRLASGISILVFPEGTRSRD
                     GKVGAFKKSGFLTALKAELPIVPVGVSGARAVLGAQGMVIRRGPVAARVGRPIPTAGL
                     GVHDRAALMDRVRREILSLRG"
     gene            10845..11903
                     /locus_tag="PLB01_01940"
     CDS             10845..11903
                     /locus_tag="PLB01_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HQQ76088.1"
                     /translation="MSTATLNEQTVLDALRKVKYPGFSRDIVSFGFVKDLTIGGGNVS
                     FRLALTTDAPAAAEVLKRECETVLKSIPGVNAVTIAVTAAAPQPPVIAGAVGRKDILK
                     DTRFVIAVASGKGGVGKSTVSANLAIALKKLGYEVGLMDSDIYGPSQQMMMGINTKPF
                     VNEEEKILPIERFGVRVMSIGFLMDVDTPVIWRGPMIFKAVEQFLGDVAWGKQDFLLV
                     DLPPGTGDAQLTLTQKVALSGAVIVSTPQDVALIDARKGLAMFQKVNVPVLGIVENMS
                     YYECPKCGNRDEIFKHGGGKRTAELLGVPFLGEVPIDPRVAIQGDSGTPIMASDPDST
                     VAKAFLRLAEQVAKAVGA"
     gene            11954..12712
                     /locus_tag="PLB01_01945"
     CDS             11954..12712
                     /locus_tag="PLB01_01945"
                     /inference="COORDINATES: protein motif:HMM:NF017945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HQQ76089.1"
                     /translation="MDGNNLLESLFRWAHVVAGILWIGHLWFFNFVNSNFVPTMDGDT
                     KKKVVPELMPRALWAFRWGAAWTWITGLVLLGLVYYNTGLLFKDPTVKTWGTGGVAVL
                     VALLFFAVYDPLVKAIAKTEIHFWLGLAVATAAVFFFSRFGGFGFRGTAIHLGAMFGT
                     IMAANVWMKIWPAQRKIITATKAGEKPDPALAAMAGLRSKHNTYMSVPLVFLMLAQHA
                     TWAASYEWALPVVVLVGWGFTYWMYKKAATVKGF"
     gene            12763..14697
                     /locus_tag="PLB01_01950"
     CDS             12763..14697
                     /locus_tag="PLB01_01950"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQQ76090.1"
                     /translation="MIPSGRFLRVGFAVVGFTCALGLVSRAATNKPPLHAKKWLAITG
                     KPLAATAGAMIFQKGGNAVDAAAAMIAATSTMWDTLSWGGETQALIYNPKTGKVIGIN
                     ALGVAPTGATPEFFRSKGMAFPPEYGPLAAVTPGTPGGIMVMLAEYGTLSLADVLAPA
                     IDMADGYPVEQQHVSTALRQRDWLLKWPESKRVLLPRPGDQPPQAGEIFRQPDLAATL
                     RKLVEAERQARAAGKSRKDAILAANDRFYRGDVAREFVRGSQEVGGLHTLEDLANWKV
                     RIEEPVKTTYKGIDVYKLDFWTQGPAMLQALNILEGADLKGMGYNSARYVHALYQAMN
                     LAFADRDFWYGDDPRVPRKGLLSKEYAAARAKTIDWAKNDPEVKPGDPYPFQGGEKNP
                     FKDFLEGWKSGAPQRPRGPQVTNQDAPSAALRPHALTFEENPLSSSPSSADPFSSDSF
                     FAGTTSVEAADAEGWVVSVTPSGGWLPAVIAGKTGIGLSQRMQSFVTDPALGPLNVLA
                     PGKRPRVTLTPTLALKDGKPYLCFSVQGGDSQDQNLLQFFLNVVEFGMTVQEAAEAAN
                     VNSYQMRSSFGAHESRPGAMMIPASLPAWVRAELVKMGYKLEPAAKTSGPLNAIFLDR
                     AHGTMWGGSSDHGEDYGIGW"
     gene            14780..15748
                     /locus_tag="PLB01_01955"
     CDS             14780..15748
                     /locus_tag="PLB01_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQQ76091.1"
                     /translation="MVRTVRRKSAGFTLIELLVVIAIIAVLIALLVPAVQKVREAAAR
                     AQMIEELGTDFCQALHGYFREFGVYPATLDDPALLAFLPAVQSPEKIAADLGFKLTYQ
                     VTRGTPGDESTWDFELCAAKGTLELCTTKTCQVTGLPGTRVTPPLPLPTAVLAQAAET
                     VTPILLAHPELIARVRPYLKQADIAGFIFDELDGNHDGTLTLGEMLENPYVAPFASLL
                     KTPGTFGPQIDARIALTHADLSGDPAFLFSYDSLEALTGFYSDKRAIAHSLDAKLEAA
                     RDAEARGDAAAKAGALRAFANEVRAQTGKALAPGHALVLQTLARTL"
     gene            15814..16377
                     /locus_tag="PLB01_01960"
     CDS             15814..16377
                     /locus_tag="PLB01_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015892934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ76092.1"
                     /translation="MDFDLDSAAAVLERTPGTLRALLGGLSDGWVRGTEGPDTFNAFD
                     VVGHLIDGEETDWIPRARIILARGEDPRFVPYDRFRHRARNAGRPLASLLDEFGSLRR
                     ANMALLASWRLTAADLDLPGTHPDFGRVALRELLAAWVVHDLGHVAQVARVMAKQYGG
                     QVGPWGAFLPVLTDRPVPRSGGSAPVS"
     assembly_gap    16603..16702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16703..17260)
                     /locus_tag="PLB01_01965"
     CDS             complement(<16703..17260)
                     /locus_tag="PLB01_01965"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HQQ76093.1"
                     /translation="MRATTGLLCLCLAPQAAFAQPAPRPGVLDKAAVVAQPTPTRTPT
                     PVNIGNFVWDDLDGDGVQDAGEPGLAGVTVQLWNPAKSQMIASAVTNGSGNYIVVAPQ
                     PGSYRVRVVLPHASDTFSTKNAGGDDQKDSDVNPSGSDLGFTDVINIASNVISITNVD
                     AGIRVFRTPTPTRTPTPVNIGNFVWD"
     gene            complement(17346..19391)
                     /locus_tag="PLB01_01970"
     CDS             complement(17346..19391)
                     /locus_tag="PLB01_01970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQQ76094.1"
                     /translation="MRFRTVLVLAFSFAGHSLFAQAPSAKPKAIDPADFDASAKPCED
                     FYQFANGAWLAAHPIPADRSRYGGFDALADRNRDVVKSILEETSGKADWPKGTPQQKV
                     SDFYATGMDAAAREKAGAAPLAPSFATIAKLKSPDDLPAVLAELHLAGANAGFGFRVA
                     PDARNSTRTIGIFNQGGLGMPDRDYYLKDDAKSKDLREAYRAHVAKVLELVGDAPEAA
                     KAEADVVMDIETKLAKVSITRVENRDPQKTYNKKTLAALNAEAPGFDFAKFLAEMGAS
                     ASTEVNVRQPGFFVGFAAYARSVPPADWRTYLRWHATRAAAPFLSKAFQDEDFAFSGK
                     KLNGTPEQEPTWRRVQAATDMALGEAVGPMYVARAFSPKAKERMRVLVENMRAALKER
                     IEALPWMGAETKAAAQRKLAAFNVKIGYPDVWKDYSALTISRDVPFAENVRRARVFET
                     KRNLAKLGKPIDRTEWGMTPPTVNAYYSASLNEIVFPAGILQPPFFYEDADDAVNYGG
                     IGVVIGHEMSHGFDDSGSQYDADGNLKNWWTADDRKAYDARTALIVKQFDAYRPLPDQ
                     SINGKLTLGENIGDLGGIKIAYAAMQKAYAGKPEAKNAKIDGFTPEQRFFLSYATIWR
                     SQYRDEAMRVQLNTNPHSPGKWRALGPPSNLPEFYDAFGCAEGTPMRRAEADRPSIW"
     gene            complement(19454..>19901)
                     /locus_tag="PLB01_01975"
     CDS             complement(19454..>19901)
                     /locus_tag="PLB01_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76095.1"
                     /translation="SADGVKIKFLDFSADRSAINDAKRPHVTVTANFLVTTAEVAEEK
                     TAKFVMYFGATDGGPATEAPDTPLPGRGRMRIKRVSPNEGTCELELMGVAAGGDLKPA
                     TAETLSIDVTTKPLISLVWAGFYVMMLGGIVALLRRARDSRQAAVA"
     assembly_gap    19902..20001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20002..21635)
                     /gene="ccsA"
                     /locus_tag="PLB01_01980"
     CDS             complement(<20002..21635)
                     /gene="ccsA"
                     /locus_tag="PLB01_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQQ76096.1"
                     /translation="MGLGSPLGFAPGTVAMWAALVFGVLTALAYWRVMRMGHAAEDFL
                     RKGKSGSGSAAAVAASPALLPFEENPDLLLARRLYYAFTFSVVLASVLLMGRLLSHDF
                     RVSYVASYSGRDLPVYYLFSTFWAGQEGSFLLWLFFGSLIGLFVLRSAKEQEPPVMIV
                     YVASFIGIVAILVKQSPFRFLGQIPPDGQGLNPLLQDPWMVIHPPVMFSGFASLSVPF
                     AFAIAALWMKRWDGWVVRAMPWALFTFVTLGTAILMGGYWAYKTLGWGGYWAWDPVEN
                     TSLVPWLATVALVHGMYLQKAREKHRKINIILAILAFCCILYGTFLTRSGVLADFSVH
                     SFIDLGITGWLVAIIVVFLFGGLGLLAFRWKQIPVVSDIDAETGKEKEEPFFSRSVLF
                     ILSVTLFSASGLVILLGTSAPILTRIGGKASQVSTSFYNVTHAPIAALMALLVALVPY
                     LSWRGETLAVVAKKAFVAAAIGLMGGGVAFAAGVHSARDLAIIAFAVFGMAANAETVA
                     LFVRRKAVSTVGGYLAHVGVSIMLVGILVSGVYETKT"
     gene            complement(21637..22032)
                     /locus_tag="PLB01_01985"
     CDS             complement(21637..22032)
                     /locus_tag="PLB01_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQQ76097.1"
                     /translation="MKRAYWAGAVLILGFLALGLTTFTKSMTPYVSFSEAKTSHRTVQ
                     VMGALEKGSTKYDQDKKVLNFTLVDPETKDVIPVSYTDVKPANFEDAVSIVAIGRYEN
                     DRFTAEKLLVKCPSKYQGEEIEKQYGKKA"
     gene            complement(22029..22160)
                     /locus_tag="PLB01_01990"
     CDS             complement(22029..22160)
                     /locus_tag="PLB01_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HQQ76098.1"
                     /translation="MSTALGYVAAVNIVIWAGIFGYLVYLDRRVRAAETRSGGENKP"
     gene            complement(22157..22819)
                     /gene="ccsA"
                     /locus_tag="PLB01_01995"
     CDS             complement(22157..22819)
                     /gene="ccsA"
                     /locus_tag="PLB01_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQQ76099.1"
                     /translation="MKSLGLISKWFLGLGMAAICWGAFLYARPAQNFVGESSRIVFFH
                     VPQAWVSTVAFFVAAWYSWRYLRKRRIEDDDAASAAARLGFLFCVLATVTGSIFARVM
                     WNSYWNWDPRETSIVLLLLVYGAYLGLRGALEDPERRAALSAAYALVAVVTVPFLVFA
                     VPRMYQSLHPDTVINTEGKVKMSSDIRLIFFSSMVAFMGLFTWMWNLDRRISRIVRER
                     SA"
     gene            complement(22882..24168)
                     /locus_tag="PLB01_02000"
     CDS             complement(22882..24168)
                     /locus_tag="PLB01_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76100.1"
                     /translation="MRRGLAILAALAAWGAPAAAQSALDWGRISVFGQVIRTKNVDGT
                     TSDYNELSGSVSIHTPPRDVDGFEFSLEARGSEYPSVTNRDPRWTVYDAWVGGRAAGG
                     KVAVRLGQMYLNDLGALGGVGGLSLEARAGKIRVGVFGGLEPKGYDPGYVQDVKKGGA
                     YVAYDGERGWRDVLGFVTIRNQNLAERNVLTTTNFIPIGREFFLYQAAEYDLTGPGGA
                     GSGGLAYFFATARWSPVRSFEIQALYQHGRSVDARSITQDQIAGRPIDAKSLDGFLFD
                     SMGGRATVEFARGWRAWAGYYQDKSNRDDVAQNRLQFGLSAMNVAGTGFDVFVSDNRT
                     NRPGSNSYDAWYFSVGRSLGSKFYLTLDYSTSLSVLQITDSGGLTIVNRPSSRRYSLS
                     GIWNMSRAFSLLLTGEQLDDDTATQNRWLLGLTYRF"
     gene            complement(24168..25613)
                     /locus_tag="PLB01_02005"
     CDS             complement(24168..25613)
                     /locus_tag="PLB01_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76101.1"
                     /translation="MRNTRLFPLGMNLHEIHLVAVLAALLAWGGPAAGQSMTGWPLAG
                     VHLNTPCTTCHKNNVYKGTSRLCGTCHLTDYQKAVNPPHASAGFPTTCDSCHQFAAPA
                     WKPASFNHNTNTTFPLAGVHVTQACATCHKNNVYKGTARDCYTCHLADYQKSVTPPHV
                     TSGFPTACDTCHQFSAPAWKPASFNHSTNTTFPLAGVHATQACTACHKNNVYKGTARD
                     CYTCHQADYQKSVNPPHLAGGFVTTCDTCHQFSAPAWKPSTFNHSTNTPFPLAGVHLT
                     QACATCHTTTPYRNAQTTCSGCHLNNYPKNYNNSVNPPHLAGGFSTTCDTCHQFSAPA
                     WSPSIFNHSTMTPFPLVGVHATQTCARCHTTMPYRSAPTTCVGCHQTDYNNSKNPSHT
                     AAGFPTSCDTCHKATDATWQQGKFNHTYFPLVSNHNVPCNQCHTTINVFTVFNCIGCH
                     TKATTDSKHKGRAGYSWTSTACYSCHPNGKS"
     gene            complement(25647..29651)
                     /locus_tag="PLB01_02010"
     CDS             complement(25647..29651)
                     /locus_tag="PLB01_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ76102.1"
                     /translation="MTAATMQARKADEPAYTRRRGRGHRASRLGLGLAVSALAAFSSP
                     AGAQGLGSLVSPGKLSRPHTKLEGLDKCQSCHEPGRQVTAARCLECHKPVAERIKAKK
                     GVHREASGKDCAGCHVEHAGIDGDLLHFTPKGFDHKGEAGFALDGLHAPLECKACHKT
                     RSYLSVSPSCASCHKDVHNGTLGATCQTCHPTNVAFKDTKKGFDHSRTKYPLTGAHAK
                     APCESCHKTKGQYRIERFGACEGCHKNPHVPALGTCTTCHVTTSFKTLAEGQKFDHAK
                     TGYPLKGRHAAVACQTCHVKPTTVVRLKYARCMDCHTDVHKGLFPAEDCAACHTEAGF
                     KPAKFDHNARTKFALDGKHATTACVACHKAAGVQARVNMPVSQRIVDFRGANAACSSC
                     HADVHKGKLGTTCQTCHGTTSFKLSAFQHPRQPEFFQGAHAGIACEKCHLPEATSVVG
                     LVKGIGPPAGSRQYKGVPFACAACHKDPHLGQVGPDCAKCHGLAVKGYKADLFVHTNS
                     KFALKGKHEPVPCAKCHAKETGKFPSGNGTAVRLTGLSTECRACHKDPHLGQLSPRCE
                     TCHDAGSFKVAAFKHPKKKETEEFFVAKHATIACAECHKRETADYPAGRGTAVRYAVP
                     VACVHCHEDVHRGALGRDCASCHNAVIWRTASRAFHKTAAFTLEGKHLAVPCASCHIK
                     GVLKGTPTGCYDCHWVRRQDDRFKTALGGDCQTCHRPITWSAVTWSHAAATTFTLETP
                     HRTLDCESCHKAQVFKGTSRDCYSCHRTDYERVQNPGHVAGGFPTDCTGCHKASSPTW
                     RGANFTHATFPLAGVHVTQPCAACHRNNVFKGTPRDCFTCHRTDYQNSKNPPHAAAAF
                     PTACDSCHKFSAPNWKDAGFNHNVTTTFTLAGVHVTAPCTACHVNNVFKGTPRDCYSC
                     HKADYQNSRNPSHTAAAFATTCDTCHKYTDPAWKPASFNHNTATTFALAGVHATQTCA
                     TCHVNNVFKGTPRDCYSCHKTDFQNSKVPPHVASGFATTCDTCHKFSDPAWRPASFNH
                     NTATTFTLAGVHATQTCATCHVNNVFKGTPRDCYSCHKTDFQNSVNPGHVAAGFPTTC
                     DTCHQFSAPAWKPSTFNHSTSTTFPLAGTHVTQLCSACHKANVFKGTARDCFTCHKTE
                     YQNSANPGHVAAGFPTTCDTCHQFSAPAWKPSTFNHSTSTTFPLAGTHVTQLCSACHK
                     ANVFKGTARDCWTCHQPDYQKSVNPPHASSGFPTTCDTCHQFSAPAWKPSSFNHSTST
                     TFPLAGVHTTQLCSACHKNNVYKGTARDCYSCHVVDYQKSVNPPHLAAGFSTNCETCH
                     LFSAPAWVPSIFRH"
     gene            complement(29648..30460)
                     /locus_tag="PLB01_02015"
     CDS             complement(29648..30460)
                     /locus_tag="PLB01_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQQ76103.1"
                     /translation="MDQAAGSVPGHARVFASPTTLLASPAKAPSAAHPDPPSPPGQVL
                     VFPRKEHAREILRFVRGEMLLHWSIALPFMTCFLTGVIVKFGYNLHSDSLVREVLLFL
                     HRVGGGCLAVFPALVILHTRRDYKKHLYNIKIGFSWTIDDLKWLFLFGPSTVSKRIVL
                     PEQRKFNAAERLNFMMVMVTYPLFVATGLFLWTTGVDHFLPWLLHISMSLVAPLLMFG
                     HIYMAVVNPSTRVGLTGMISGRVDREWARHHYQSWYREHFEDDGTPKQSRPS"
     gene            complement(30653..31351)
                     /locus_tag="PLB01_02020"
     CDS             complement(30653..31351)
                     /locus_tag="PLB01_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQQ76104.1"
                     /translation="MNARSPAPLLGAALAILRKDLLAEWRTRIAVNALFLFAFAVLVL
                     VGYAVGPTRLAPEDRPTVNAVLLWIVIFFSAMTGLARVFVKEEEGGTAAALRLAAPPA
                     AVLLGKYLVNIVLLFAVTAFIVPLFLVLTSFEIACPTLFVVLLVLGGLGLAGASTFTA
                     ALVSKAAAKGALFAVLSIPLLLPPLIAAVSGTQVAARAADFSAGLDFVRVLVAYDGVV
                     TTAAFALFDAVWRE"
     gene            complement(31348..31974)
                     /locus_tag="PLB01_02025"
     CDS             complement(31348..31974)
                     /locus_tag="PLB01_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ76105.1"
                     /translation="MRLVVDGLTRSYGARRIFGPLSFEVPEGRVLGVAGSNGSGKTTL
                     LKTLSGLIRPSGGSTKIFLERESTGAGQEPRDVPWCIGWVAPDLALYGELTAEENLTF
                     FQSVHGLPASPGRARALLEGVGLSEHSHSKKSLSSLSTGQRQRVKLAYATLHDPAVLF
                     LDEPSSNLDADGVEVVARVVASQRSRGVAIIASNDPRDLALADETVSL"
     gene            31999..32661
                     /locus_tag="PLB01_02030"
     CDS             31999..32661
                     /locus_tag="PLB01_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HQQ76106.1"
                     /translation="MLQRFLGVLVFLAISLWKATLRITIVGEEHRQAIRAKGKKPLHA
                     IWHQRMVGGILAHRGEGYVTMASKSEDGEIIATFLKYWGFVAARGSSSRGGDVASAEF
                     LEALKVAPGGALTPDGPRGPARTCKRGILVLAEQGNGLVLPSASSSSRPRFLNSWDRF
                     LLPMPFSRCVVVFGPPLERAADEAEGAFLARAASAIDAATDEADRLCGVVGAPRERMK
                     RA"
     gene            32658..33641
                     /locus_tag="PLB01_02035"
     CDS             32658..33641
                     /locus_tag="PLB01_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQQ76107.1"
                     /translation="MTLPVTVADVEAARRRIAPHVHRTPVLGSSSLDARVGARLFFKC
                     ETFQKVGAFKARGAFSRLTLLSPEERRRGVVAFSSGNHAQAVALAARTLGISATIVMP
                     HDAPALKLAATRGYGAAVKVYDRARESREAIAKDIVEREGRVLVPPFDDPAVIAGQGT
                     AALELLEDVPDLDLVLAPCGGGGLLSGVAVAATALRPGIGIWGVEPEAGDDFRRSLEA
                     GHPVEIPVPATIADCLQTTRAGVHTFAVVSALAKGILTVSDLDLRVAMSVLFTRMKLV
                     VEAGGTAGFAALLAGKVPDAAGRRVGVVLSGGNVDPEQFGRLLTGLPEGSV"
     gene            complement(33689..34258)
                     /locus_tag="PLB01_02040"
     CDS             complement(33689..34258)
                     /locus_tag="PLB01_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQQ76108.1"
                     /translation="MPRRPIVLACAAAAFLAAAPGAAQSNSDPDAQWGVHGGVVAGAD
                     VDGTHPSAGLHWRPRLTGALGLEVSVGYERLSWPGAGETVEADHVPVEGSLLFFFFFS
                     RRVQPYLLAGIGYHWVNPYVGNPPAGTAYSSQNLFALHAGAGVDVRAGEKLSFWIDGR
                     WTFLDVAAVKDLGLKSDTLRVTAGVNVAF"
     gene            34313..>37110
                     /locus_tag="PLB01_02045"
     CDS             34313..>37110
                     /locus_tag="PLB01_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76109.1"
                     /translation="MAGRTWKDDLPSLILALAAQLGLVRAVDWARANTATHDLARSLV
                     ENRFYFAPQIGSLLVVFLFARLVVNALNLRGVAIGDWLRSSALLLVLGLGCLVAAHLD
                     QILALAAEILGTPALQAAAQDVAASSFSRVLGRTATLRFAIDLAGLLCCLFAIRLGKP
                     SLPDAQLARGYASAKDWVRAGDAYLKAGDVRRARRMFKKGRSWARLAALEMRDGNPAE
                     AARLYQDAGDAWLWEASRAWLAADNPAAAEETGLKALQLARTGGRWDRLAEIAEALGD
                     NGTLAEACRKLAEATTSGPVRSALWKRAGEAYRVAGRPGEAAEAFRAAQEYLLAGELF
                     VESGRTTEAVQSFEKSGALTKAALAAARAGDEKSAQELLAREAEARGDLVSAADAWRR
                     AGQPLRAAGFYERSGDFVKAGDAWKDAGRPERAAALYQKGGANVAAAEAYEATNQHEK
                     AATLYVELKHYDKAAALFRAAGRLPAAAAALQAGHQYDEAAQLFHRTGRSLEAARCAL
                     LGGHREKAWEYLTSVPRNEEGLGELFHDLARAHLAANEPKDAVHVLREFLPRGPVEAD
                     NLPLHMTLLAALRAAGDSSEGVELGRVAAFDPSVLEGSSVAPQLPFGEVEVDASMPSA
                     LIRTPAVHKSGPYAAVTRSPVTQKVQPPRLAIPGPSPTPAPAPAAAQPGPPPPFRTGS
                     GLPETPEDRYEILAELGRGGMGVVHKALDRKLDRFVAVKILPWQLRGDETAMRYFKRE
                     AKAIAALKHPNIVALYDYGDGFGSLYLAMEYLEGPNLQKLIKKDPERVRKNWRNWFTQ
                     ATRGVAAAHAKGILHRDLKPANLILDEHGTLRILDFGLALPQADAGGTSKLIGSPAFF
                     PPEVLRGEMPSPASDVYSLGAAFYTLATGRWPYVGEDVLVARLERDPDDPRPYAPFLN
                     EEEIRILMKTLARHRPER"
BASE COUNT         5628 a        12231 c        13264 g         5787 t
ORIGIN      
        1 cagccccttc ggcgcgcgca acgccgggag ggacgcccgc gccggccgct ctcgcgggcc
       61 gcttgaagat cctcgcgctg ccgtacgcgc gcgttctcgc cgtcgtggac gccgccggaa
      121 aggcggtcgc gctcccgaag gacgcgacga cgcctctcct cctgcgcgac ctcgcgcccg
      181 gcacgtaccg ggtggcgctc gccggcccgg acgggtcgcg ccgggtggag cggcgcgtcg
      241 acgtcgctgc gggagcggcc gagctcctct cggagccgtt cgagacgcct tcctcgctcg
      301 cccagcttct cgggaccgga ggttcgaagc catgacgaag ccgttcttcc gggggattcc
      361 ggccctcgcg gtccttctcg ccgcgtgggc gccgccggcg gccgcccagc tcgattcggc
      421 cctcctctcg aagctgcgct tcaacctcac gaaccccggc ggaaagtcgc tcgcgatggg
      481 cggcgccttc tccgcgatcg ccgacgacgc gacggcggcg ctcgcgaatc cggcgggcct
      541 cggcctgatc tcgtcgatcg agatcgcagt ctccggcaag cgcgtgaacg agacgctcgc
      601 cctgtcgacg gcccgttcgg tggcgacggg cagtctcgtg acgccctacc cgccggtcac
      661 gacgtcgtcc gtgggcctcg acgcgagctc gacgggtgtc gagttcgccg gcgtcgtcgt
      721 cccgatctcg cgccgcctcg tggccgcgct cacgtacgcc gaaaacctcc gtttcgaggg
      781 cgacgcggga cccggcggct atcagtacct cgagctgcgc gacaacaggt ccggggggac
      841 cacgcgccgg gactttctct tcgggtaccg cgagtacggg tcggcaaagc tctcgaaccg
      901 gctcctcgcg gcctccctgg cgtatcgcgt cacggagagc ctgcggctcg gcggcggcgt
      961 cacgctgaac cgttccacgt tcgacctcga gggcgacgcc ggcgggccgc accggatcgt
     1021 cagccagacg tacctctcgc cgaccgcgca ggagacccgg acggtcaccc tgggggtgga
     1081 gggcctgtcc ggcacgcagg tcggcttcgt cgcgggcgtc cacgcggacc tcctgtccga
     1141 cgggcggctc acgttcggcg cggactaccg atggaccccc gccgccgacg cggcgctcgt
     1201 gatcgccggc gacgtgccgc ccgcgctcgc gtcccagacg tcgcgcgcgt tcaagttccg
     1261 ggtgccgagc gacgcgtccg tcgggctggc ggcgcacccg atgccggggc tgacgatcgc
     1321 cgcggaggtc cagtgggtgc gctacggcga catcttcgac cgggcgctgc ccgtcgtttc
     1381 gtacgccggc ctcgcggggc cgttcccggg cgtgcccgtt accggcgtcc tcgcggacct
     1441 ctcgcccgcc ccgaacgtct tcgtcccgcg gctcggcttc gagtacgtcg ccggtggaaa
     1501 ggacgtccgg atggcgcttc gcgccggctg gcaccgcgag cccgcgcacg gcgtcacggc
     1561 gaacctcgcc gcgcgcgacg gctccggcca gccctacccg atcacggacc cgccctactc
     1621 cgagagcgtg gccgcggttt tcgacggcgg aaaggcggac gaccggttca cgggagggct
     1681 cggcctcacg gtgtcccgcg cgctctccgt ggacttcggg ttcgacgtgg ggcgcgactc
     1741 gcagcagttc gccgtgagcc tcttctaccg cttctaggcg cccgcggtcc ccggggcgcc
     1801 aggctcgtcg cgcgtctcca tcgcgctcgc gtcgcgcgcg ccgtcgcgcg tcgcgtcgtc
     1861 gagcgcggcc tccgcgcgcc ggatgaggtc ctcgatcgtc gtcttctcga gcggccgccg
     1921 ggtcgcgatg cccgcggacg ccgtgacgcc ggccgccttg gccgcgaaag cccggatgcg
     1981 gtcgacggcg gcgcgcgtgc tcgattcctc ggcttccacg aggagcgcgg cgaaggaatc
     2041 ggggccccat cgggccggta cgtcgatgcc gcggatcgcg cgggcgatgc cctggccgta
     2101 agccgcgacg atctcgtcga cggccgcgga atccttctcc ttgcggatcg cggcgaaacc
     2161 gtccagctcg aggaggacga gcgcgagggg tcgccgcgtg cgctcggcgc ggaagagctc
     2221 gtcgcgcgag cgggcgagga acgttccgcg gttggggagg ttcgtgaggg cgtcggtcgt
     2281 ggcgcgcgtg aagagccggc gctcctcgtc cgccgcagtg gggagaagga gggccgtgaa
     2341 ggccaggaac gccgtgccga gcaggacacc cgaggcggag gacggttccg gaagcctgcg
     2401 tgacgcgatc tcggcgacgc ctccggcgag gaggatgccc gtcgccgcga aggcgaggaa
     2461 cgtgtcccgg cgccggttct ccttccgtgc gagcacccag gcgacgccgg cgcccacgat
     2521 gaggagcgag gccaccgcga ggctgtcgag ccagcccgcg gacgcgggca gggcgagggc
     2581 gagcgccgtc ccgaggacgg ccggcgccag gatcgcgagc cgcagccccc gagcgccgag
     2641 gccgaggatc cggagcagga aaaggaaccc gatcgcgggc aggaggaacg cgagggcgcg
     2701 cacgagccgt ccgaggatct cccgcgagat ggggacgggc acgggcgtca ggagcggcgt
     2761 cgcgagccag aacgcgaccg cgaacgcgag gacggcgtag aggccgagct ccacgcgcgc
     2821 gcggttccag cggagcagga gcacggcgag gacgcccagc gacgccgcga gcagcgcgca
     2881 gaacagcacg aggacctgtg gcagcgtgaa gagcacggtt cgaggctaat tctacgcgcg
     2941 gggccgtcgc cttgtcaggc gcgcgcctcg aagtcccgga tcgtgctgcc gaggccttcc
     3001 tcgagggcga ccttcggctc ccagccgagc gtcgccttcg cgagggaaat gtcgggccgc
     3061 cgctgcctcg ggtcgtccac gggaaggggg cggaagacga tcggtgcggg cgttcccgtg
     3121 agccgcttga tcgtctcggc gaactggaga accgtcatct ccgccgggtt gccgatgttc
     3181 atcgggtcgc ggtagccgga cgtgaggagc cggtggatcc cctcgatgag gtcggagacg
     3241 taacagaacg aacgggtctg cgaaccgtcc ccgaagaccg tgagcggctc ccgacggagc
     3301 gcctggccga tgagcgacgg cacgatgcgc ccgtcgttcg gccgcatccg ggggccgtac
     3361 gtgttgaaga tccggacgat ccgcgtgtcg acgtcgtgga agttccggta cgcgatcgtc
     3421 atcgcctcgg cgaaccgctt cgcctcgtcg tagcagccgc gcgggccgac ggggttcacg
     3481 tttccccagt acgtctcggg ctgcgggtgg acgagcgggt cgccgtagac ctcggacgtc
     3541 gaggcgagga ggaagcgggc gcccttggcg cgggcgaggc cgagcgcctt gtgcgtcccg
     3601 agagcgccga ccttgagggt ctggatcggg atctggaggt agtcgatggg cgaggcgggg
     3661 gacgcgaagt ggaggatcgc gtccaccggc ccgtcgaggt agatgtactc cgtgacgtcg
     3721 tggaagacga agcggaactt ccggtttccc gcgaggtgag cgatgttccg cacgtcgccc
     3781 gtcacgaggt tgtcgaggcc gacgacgtcg tgaccctcgg cgaggaggcg gtccgtgagg
     3841 tggctgccga ggaaccccgc ggctcccgtg acgacgacgc gcatcaggcg cgcccgagcc
     3901 cgacgtagtt ccagcccgcc ttcttcatct ccttcggctc gtagaggttc cggaggtcga
     3961 cgaagttctt cgccttcatc gtcttctcga tccgggggag gtcgagcttg cgaaactggt
     4021 tccagtccgt ggcgaggacg aggacgtcgg cgcccgcgat gcaggcgtac tggtcctcgg
     4081 cgagcttcag tcccggcatc tccctgcggg cgttctccat cgccgcgggg tcgtaggcga
     4141 cgacgtcggc gccgcgggca aggaggtcgg acgcgagctt caggctcgcg ctctcgcgga
     4201 tgtcgtccgt ctcgggcttg aacgcgatgc cgaggagcgc gacgcgtttg ccgtcgacgc
     4261 tcccgcacag cccctcgacc ttgtcgatca tgcgggcctt cacgcggtcg ttcgcggaga
     4321 gcgtcgcctc catgagctcg aacgtgtagc ccaccttgcg ggcgaggtcc acgacggcgg
     4381 aggagtcctt cgggaagcac gagccgccgt agccggggcc cgggttcagg aactgcggcg
     4441 cgatgcgctt gtcgaggccc attccgcgcg cgacgtcctt gacgtcggcg cccatcttct
     4501 cgcacatcat cgcgatttcg ttgatgaacg tgatcttcag cgcgaggaag ccgttcgagg
     4561 cgtacttgat gagctcggcc gactcgacgt ccgtcacgac gaacggcacg cccgcgatcg
     4621 cgagcggcga gtagacgtcc ttcacgatcg cgatcgcttc ttcgtcgcgc gagccgatca
     4681 cgacgcggtc cgggtgcatg aagtcctcga tcgccgagcc ctcgcgcagg aactcggggt
     4741 tcgagacgac cgagaacttg tatttcccgt tcttcttgag gatctcttcg atctgcgcgc
     4801 cggttcccgt ggggaccgtg gacttcgtga cgacgacctt gtagccgttc atgtgctcgg
     4861 cgatcgtctc ggcgacctgg aagatgaacg aaaggtccgg agagccgtcg tccttgggcg
     4921 gcgtcccgac ggcgatgaag atcacgagag agcgttcgat cgcctcggcg acgttcgtcg
     4981 agaaccggag gcggcccgcc ttctcgttct tgtggatgag ctcctcgagg cccggctcgt
     5041 agatggggga gacgcccttg ttcaggagcg cgatcttgct ctcgtccttg tcgatgcagc
     5101 tcacgtccat gccgaaatcg gacaggcagg cgcccgtgac gaggcccacg tagcccgtgc
     5161 cgaccatgca gatgttcatc ggaaagaagc ctccagcggg ggaatcccgg ggagggagat
     5221 tgtacccgcc ggaggccttc gagacctggt ttcagggtct gagaagaacc ttcaggactc
     5281 ccgggcgccc agcctcgcgg agcgcgcgaa ccgcctggcc gagcgggaat tccgacgaaa
     5341 ggaggaagag gacgtccgct cttttcattc gaaggaattc gagcgccggt tcgaagcgcc
     5401 cgcagcgtga acccacgacg gtgacctcgt tcacgacgag cggcgcggcg tcgaaacggg
     5461 cgggcgcgtg atgcgtcgac ttccagacga tcgttccgcg cggtttcacg agagccaggg
     5521 cgcgcggcat ccccgcgggc gagcccgtgg cctcgacgac gaggtcgaac gacgcggcct
     5581 tcgctcgcgt cgtgcgaccg agaagagcga cgtcggcgcc cgcagtcccc agcacggccg
     5641 cagcgaggcg cccgagcttg ccgggcccga ggacagccgc gcgcgttccg gacggcaccg
     5701 cgacctggtc gaggatctcg caggcggccg cgagcggctc gacgaagacg gcctcttcgt
     5761 cggcgacgcc cggcggaacc tcgaagagat tcgcctcggg aagggtcagc cactcggcgt
     5821 gcgcgcccgc gtggccgcgg atgccgagga ccgtgcgcgt cgggcagtgg cgcccaagtc
     5881 cctgcgcgca ccatgagcac agtccgcacg cgaggttgat ctcgccgacg acgcgcttgc
     5941 cgagccacgg cgacgccgcc ggcccttcga cgatgccgac gaactcgtgg ccggggattc
     6001 ccgagaagcc gtggtatccg cgcttcagct cgagatcggt gttgcagatc ccggaaagaa
     6061 ggatccggac gagcgcgaag cccttccgcg gcgagggccg ccgcacgtcg cggacagcgg
     6121 ccctgccatt gtcgaagacg agagcgcgca tggtgcctct caggagccgg gcttgcgggc
     6181 gccgcccagc gggcggcgga acatgtcgaa gagttcgcgt cgcgagacgg gcttcggagg
     6241 ctcgacgggc ggctgcggag caggcgcctc cgccggctgg taggcgcggg ccgcctcggc
     6301 gggaaagagg cgggcgcgca ccgtgtccca gacgatttcg ccgaggccga gctgcgggga
     6361 gaagccgagc gtctgccagg ctttctccat gtcggcgatg cggggcgggc gcgccgcatc
     6421 gccctgcacg gtcagccggc actcgagagc cgcgcgcacc gcgagtgcgc gaacgacctc
     6481 ggacggcttc acggcgaggc cgaagccgag gttcacgatc tcgccgatcg ggcgctttcg
     6541 cagcgcgaag agggtgccgc gcacgacgtc ctgcgggtgc acgaagtcac gcggcgcgtc
     6601 gtccggcagg aagacctcct cgccggcgag gagagcctcg agggcgtcga tggggaagcg
     6661 cgagggcccg gcgcccggtc cggccgcggc gaagacgcgc aggatcacga agggaagccc
     6721 cgacgcgaga aacgcttcct cctcggcggc cttcgcgagg agatcgcggt cctcgcccgg
     6781 ttcgatcggc tcgtcctcgc gcgcgcggag gcccgtcgcg tcccgggggc cgtagagcgc
     6841 ggcgtccgag agatggacga cgacgccgac gccggcttgc cgggaggcct cgacgagcgg
     6901 gccgaggccc gcggagtcga gcgggaggcg catcgcgttg acgacggcgg cggggcggtg
     6961 ctcggcgaaa accccctcga ggaggtccgc gtccgttccg tcccctcgga cgaccgtcag
     7021 gcgcgggtgg cgcctcacgg aggcggcccg ttcttccttc agcgcgcggc cctcgcccgc
     7081 gtcgtcgaac gtgtcgagca ggaagacgtc ctcgcccgca ccgagaagct ggcgggcgat
     7141 ggcggcgccg agaaatcccg cgcctccggc gacgaggacg cggctcacgg cgccggggtc
     7201 gggacgggcg ccttctgcgc gtcctcgtag cgcttctgga tccccgactt ctgccggtcg
     7261 cggaccagcc agcgcagcgt cttgctctcg ttgatcccga cgaggatgtc gttgatcgcg
     7321 tccgcgacgg acgggtccgc gatgaggcgg cctgcggtcc cgtccgagga gtcgagcttc
     7381 gcggcgacgt tcgcgaggtg cgaggagagc ttcctcaggt cgtccgtgaa ctccttcgcg
     7441 aacgcctcgt ccttgacgag cttcgggagg agcccgcttc cgccggagag gtccttcgag
     7501 aaggacgcga gagagtccgc ggtcgtcttg agggagtcga ccatcgcgaa gaacttcttc
     7561 ttcccttccg ggtcggagag gagcgcgggg acgaggccct cgcccgtggc ggtgcccttc
     7621 tcgaccgtcg cgaggatgcg gttgagcgag gccgtcgcgc ccttgagttc cgcgaggacg
     7681 tcgtccgccg ccttctcgtc gtgcagaagc cgcccgacga ggccccggcc ctgacggacg
     7741 tcgacgacga ggccgttcag gttgtcgagc gtctggcgga gcgacttcga gagctccgcg
     7801 ccgttcttgg tctccgacgt gatctcgccg aggaaaccct cgcccttttc cgtgcggccg
     7861 aggatgttct tgagcgactt cgagatcgcg acgacgttgt ccacgagatc gcccgatccc
     7921 gcgaggatct tgtcgagctc ggcgccgcgg aacgcgatga tctcgtggtc gggatcgagg
     7981 tcgggcttgt cgggcgagcc gggcgtcacc tcgaggtact tgtcgccgag gaggccgatc
     8041 gtcttgatct ccgcgcggct gtccgtcttg acgaggtgct ggtaccggcg ctcgatgtcg
     8101 aaatggacgg tcaggtcctg gccgggggca cgcgggacgt cgatgtcgcg caccgcgccc
     8161 acgacgacgc cggcgaggcg aacctggttg cccggcagca gcccctggga gttggggaag
     8221 cgcgcgaaat acgacgtgcg cctcacgaag aacccctttg tcccgccgat gaacagcagc
     8281 gcccccgcga ggaggatgaa cgacgcgaag aggatgaccc cgacgcgcgt cttcgtccgg
     8341 atgtcggctc tcaccatcgg gaaggctgct ccgcgcccat catgccaaag actgcccggc
     8401 cgagaggaat tccctgagtt cgggcacgtc cgaggccttc gcctcctccg gcgtgcccgt
     8461 gaaggcgaac cgggcgttct tgaggaaggt gatgcggtcc gcgacgaaga gcgccgacac
     8521 gatgtcgtgc gtcacgacga ccgacgtcgt cccgaggcgc gtgttcaggt ccttgatgag
     8581 ccggttgatc gtcatggacg tgacggggtc gaggccggtc gtgggctcgt cgtagaggag
     8641 cacctcgggc ttgagagcga ccgtgcgcgc gagcgagacg cgcttcttca tcccgcccga
     8701 gagcgaggag ggaagcaggc gctcgacgtc ccttccgagg ttcacgaagc cgaggacctc
     8761 cgcgacgcgt tcggaaatgc ggtcttcggg ccagctcgtg tgctcgcgca gggcgaaggc
     8821 gatgttctcg aagaccgtca tcgagtcgaa gagcgcgccg ctctggaaga gcatgccgac
     8881 cttcttgcgg acggggacga gggattcctc gggcaggtgc gcgatctcct cgccgtccac
     8941 ccacacctct cccgcgtcgg gctgctcgag gccgttcatg tgccggaggg acacggactt
     9001 gccggtgccc gagcccccga ggatcacgag cacctcgccg cgcctgacct cgaggtccac
     9061 ctcgtcgagg accttcttct ggccgtaggc cttggacacg cccttgaact cgatgatcgg
     9121 cgcccccgcg ggcatccccg tccggaggat gcgcatgacg ccggtctcgg agagcggtct
     9181 cacttgaggc ccagggggag caggaggaag agcttcgtca ggaagaagtc cgacatgagg
     9241 acgacgatcg ccgcgatgac gacggcgacc gtcgtcgcgc gcccgacgcc gtcggcgccg
     9301 cccgtcgcgt tcaggccgtt gtagcagccg atgatgccga tctcgaatcc gaagaacggc
     9361 gtcttgaaga ggccgtggag gatgtcgttc acgtgcacgg actgcgagac cgtggagagg
     9421 tagaacgagg cgccgatctt caggtcgacg acggcgatga agaggccgcc gaccatcccg
     9481 atgaagtccg cgaggagggt caggatgggc agcatgagcg tcacggcgag gacgcggggg
     9541 aggacgagct ttcgcgccgg ggaggcgccg agggcgcgca gggcgtccac ctgctcggtg
     9601 acggccatcg tcccgagctc cgccgtgatg ccggcgccca cgcgcgccgc gaccatgagc
     9661 gccgtgagcg aggggccgag ctcgcgggtg atcgagagga cgacgacgcg gcccacgaag
     9721 agcttgccgc cgaactgcga gagcgcggag gccgtctgga gcgcgaggac catgcccgtg
     9781 aagagagccg tgaggttcgt gatcgagatg gactgaacgc cgagggcgtc catctggcgg
     9841 aggatctcgg acgtctccca cttctcccgt gacgcgcgca cgaggtcccg gaccagccag
     9901 gcgacgccgc cgagatggaa ggagacgttc ccgaaccagc gcctgagacg cgccatcttc
     9961 cagagcggct ccttgaggac ggcgatgtac tgggtcgtcg agtcgtactg gggctgggcc
    10021 acgggaggat tctacggggc gggccccggc tcgttacact cgcggcgtgc cggccggcct
    10081 tcgtgacgcc ctcgtgacga cctggggatg ggcgaacatc gccgtctcga cggcgctctt
    10141 cggcacgctc gccatcctga cctcgtgggt gccgccccgc ggccggctct acctcttctg
    10201 ggcgcgccac tgggcgaaga gcctcctctg gctcaaccgc attcccgccc ggatcgagat
    10261 cgccgaggaa gccgccgccc tcccgcaggc gatctacatg tcgaaccacg agtccgggat
    10321 cgacatcctg ctcctcctcc tcgcgatccg gcaggacgtg cggttcctcg ccaagcgcga
    10381 gctcttcttc gtgccgttca tgggctggtc catgtggctc gcgggcttcg tgcccgtgga
    10441 ccgccgccgg acggacaagg cccgggacgt cctttcgagc ctggaccgcc gcctcgcctc
    10501 gggcatctcg atcctcgtct tcccggaggg cacccgctcg cgcgacggga aggtgggcgc
    10561 cttcaagaag tccgggtttc tcaccgccct gaaagcggag ctcccgatcg tcccggtcgg
    10621 cgtgtccggc gcccgcgccg ttctcggcgc gcagggcatg gtcatccggc gcggcccggt
    10681 cgccgcgcgc gtcggccgcc cgatcccgac cgccgggctg ggcgtccacg accgcgccgc
    10741 gctcatggac cgcgtgcgcc gcgagatcct gtccctgcgg ggttgagccg gcgggccggc
    10801 gccccgtttg tgagatcctc gttcaccgag aggaaacctt tatcgtgtca acggcaacgc
    10861 tcaacgaaca gaccgtcctc gacgcgctcc ggaaggtcaa gtaccccggc ttttcgcgag
    10921 acatcgtctc cttcggcttc gtcaaggacc tgaccatcgg gggcggcaac gtctccttcc
    10981 ggctcgccct cacgaccgac gcgccggccg cggccgaggt cttgaagcgc gagtgcgaga
    11041 ccgtcctgaa atccatcccc ggcgtcaacg ccgtgacgat cgccgtcacg gccgccgcgc
    11101 cccagccgcc ggtcatcgcg ggcgccgtgg gccgcaagga catcctcaag gacacccgct
    11161 tcgtgatcgc cgtcgcgtcc ggaaagggcg gcgtcgggaa atccacggtc tcggcgaacc
    11221 tcgcgatcgc gctcaagaag ctcggctacg aggtcggcct gatggactcg gacatctacg
    11281 gaccctcgca gcagatgatg atgggcatca acacgaagcc gttcgtgaac gaggaggaga
    11341 agatcctccc gatcgagcgc ttcggcgtgc gcgtcatgtc gatcggcttc ctcatggacg
    11401 tcgacacgcc ggtcatctgg cgcgggccga tgatcttcaa ggcggtcgaa cagtttctcg
    11461 gcgacgtggc gtggggcaag caggatttcc tgctcgtcga ccttccgccc ggcaccggcg
    11521 acgcgcagct cacgctcacg cagaaggtcg cactctcggg cgcggtcatc gtgtcgacgc
    11581 cccaggacgt cgcgctcatc gacgcgcgca agggcctcgc catgttccag aaggtgaacg
    11641 tcccggtcct cgggatcgtc gagaacatga gctactacga gtgcccgaag tgcggcaacc
    11701 gggacgagat cttcaagcac ggcggcggga agagaacggc cgagctcctc ggggtcccgt
    11761 tcctcggcga agtcccgatc gacccgcgcg ttgcgattca gggggattcc ggtacgccga
    11821 tcatggcgtc ggatcccgac tcgacggtcg cgaaggcgtt cctgcgcctc gccgagcagg
    11881 tggcgaaggc cgtcggcgcc tgagcggtct ccggcgttaa gatcgggtct caacacgtaa
    11941 cggaggacat tcgatggacg gcaacaatct cctggaatcc ctgttccgct gggcccacgt
    12001 cgtcgcgggc atcctgtgga tcggccatct ctggttcttc aacttcgtga actcgaactt
    12061 cgtcccgacg atggacggcg acacgaagaa gaaggtcgtg cccgaactca tgccccgcgc
    12121 cctctgggcg ttccggtggg gcgccgcctg gacgtggatc acgggcctcg tactcctcgg
    12181 actcgtctac tacaacacgg ggctcctctt caaagacccg accgtgaaga cctggggcac
    12241 cggcggcgtc gccgttctcg tcgccctcct cttcttcgcg gtgtacgacc cgctcgtgaa
    12301 ggcgatcgcg aagaccgaga tccacttctg gctcggcctc gccgtcgcga cggcggccgt
    12361 gttcttcttc tcgcgcttcg gcggcttcgg tttccgcggc acggcgatcc acctcggggc
    12421 gatgttcggg acgatcatgg ccgcgaacgt ctggatgaag atctggcccg cgcagcgaaa
    12481 gatcatcacg gccacgaagg cgggcgagaa gccggacccg gcgctcgcgg cgatggcggg
    12541 cctcaggagc aaacacaaca cctatatgtc cgtgccgctc gtcttcctga tgctcgcgca
    12601 gcacgcgacg tgggccgcga gctacgagtg ggcgctgccc gtcgtcgtcc tcgtgggctg
    12661 gggcttcacg tactggatgt acaagaaggc cgcgaccgtc aagggattct gagcgcagga
    12721 taggatcgcc tccgggccgt cgtgaacggc tcggaggcgt ccatgatccc gtcaggccgg
    12781 tttctgcgcg ttggtttcgc cgtcgtcggc ttcacgtgcg ccctcggact ggtttcccgg
    12841 gccgccacga acaagccccc cctccacgcg aagaaatggc tcgcgatcac gggaaagccg
    12901 ctcgcggcga cggcgggcgc gatgatcttc cagaagggcg ggaacgccgt cgacgccgcg
    12961 gccgcgatga tcgccgcgac ctcgacgatg tgggacacgc tgtcgtgggg cggcgagacc
    13021 caggcgctca tctacaaccc gaagaccgga aaggtcatcg ggatcaacgc gctcggcgtc
    13081 gcgccgaccg gggcgacgcc cgagttcttc cggtcgaagg ggatggcgtt tccgccggag
    13141 tacggcccgc tcgccgccgt cacgcccggg actccgggcg ggatcatggt gatgctcgcc
    13201 gagtacggga cgctctcgct cgccgacgtc ctcgcgcccg cgatcgacat ggcggacggc
    13261 tatcccgtcg agcagcagca cgtctcgacc gcgctccggc agcgggactg gctgctgaag
    13321 tggcccgagt cgaagcgggt gctcctgccg cgccccggag accagccgcc acaggccggc
    13381 gagatcttcc ggcagccgga tctcgcggcg acgctgcgca agctcgtcga ggccgagagg
    13441 caggcgcgcg cggcgggaaa gagccggaag gacgcgatcc tcgcggcgaa cgaccgcttc
    13501 tacaggggcg acgtcgcccg ggagttcgta cgcggctcgc aggaggtcgg cggccttcac
    13561 accctcgagg acctcgcgaa ctggaaggtc cgcatcgagg agccggtgaa gacgacgtac
    13621 aaggggatcg acgtctacaa gctggacttc tggacgcagg gccccgcgat gctgcaggcc
    13681 ctcaacattc tcgagggcgc ggacctgaag ggcatgggct acaacagcgc gcggtacgtc
    13741 cacgcgctct atcaggcgat gaacctcgcg ttcgcggacc gcgacttctg gtacggggac
    13801 gacccgcgcg tcccgaggaa gggactcctc tccaaggaat acgccgctgc gcgcgcgaag
    13861 acgatcgact gggcgaagaa cgaccccgaa gtgaaaccgg gagaccctta tccgttccag
    13921 ggaggcgaaa agaatccgtt caaagatttc ctagaaggtt ggaagagcgg ggcgccccaa
    13981 cggccgcgcg gcccgcaggt caccaatcag gacgcgccgt cggccgcgct ccgcccgcac
    14041 gctcttacct tcgaagagaa tcccctctct tcctctccct cttcagcaga ccctttctct
    14101 tccgattcct tcttcgccgg cacgacgtcc gtcgaggcgg cggacgcgga gggctgggtc
    14161 gtctccgtca caccctcggg cggctggctg cccgccgtga tcgcggggaa gacgggcatc
    14221 gggttgtcgc agcggatgca gagcttcgtg accgaccccg cgctcgggcc gctcaacgtc
    14281 ctcgcgcccg gcaagcgccc gcgcgtcacg ctcacgccga cgctcgcgct caaggacggc
    14341 aagccgtacc tctgcttctc ggtccagggc ggggactcgc aggaccagaa cctcctgcag
    14401 ttcttcctga acgtcgtcga gttcggcatg acggtgcagg aggcggcgga ggccgcgaac
    14461 gtcaacagct accagatgcg gagttcgttc ggcgcccacg agtcgcggcc cggcgcgatg
    14521 atgatccccg cgtcccttcc ggcctgggtg cgcgccgagc tcgtgaagat gggctacaag
    14581 ctcgaaccgg ccgcgaagac gtccggcccc ctcaacgcga tcttcctcga ccgcgcgcac
    14641 ggcacgatgt ggggcggctc gagcgaccac ggtgaggact acgggatcgg gtggtgaggc
    14701 cgcggcggaa ttcctgccga gacctgatct caacggcgta agatccccat tccctcgacg
    14761 gggaaggagg tcttccatga tggtccggac ggtccgacgg aagtctgcag gcttcacgct
    14821 catcgagctc ctcgtcgtca tcgcgatcat cgccgtcctc atcgccctgc tcgtgcccgc
    14881 cgtgcagaag gtccgcgaag ccgcggcgcg ggcgcagatg atcgaggagc tgggcacgga
    14941 cttctgccag gcccttcacg gatacttccg cgagttcggc gtgtacccgg cgacgctcga
    15001 cgatcccgcg ctgctcgcgt tcctgcccgc ggtccaatcg cccgagaaga tcgccgccga
    15061 cctcggcttc aagctgacgt accaggtgac ccgcggcacg cccggcgacg agtccacgtg
    15121 ggacttcgag ctgtgcgccg cgaaggggac gctcgagctc tgcacgacca agacgtgcca
    15181 ggtgacgggg ctgcccggca cgcgcgtgac gccgccgctt cccctcccga cggccgtgct
    15241 cgcacaggcc gccgagaccg tcacgccgat tctcctcgcg cacccggaac tgatcgcccg
    15301 ggtccggccg tacctgaagc aggcggacat cgccgggttc atcttcgacg agctggacgg
    15361 gaaccacgac ggcacgctca cgctcggcga gatgctcgag aacccgtacg tcgcgccctt
    15421 cgcctctctc ttgaagactc cggggacctt cggcccgcag atcgacgcgc ggatcgcgct
    15481 cacgcacgcg gatctgtcgg gcgaccccgc attcctcttc tcgtacgact ctctcgaggc
    15541 gctcacgggc ttctacagcg acaagcgggc gatcgcgcac tccctcgacg cgaagctcga
    15601 ggccgcgcgg gacgcggagg cgcgcggaga cgcggcggcg aaggcggggg cgctccgggc
    15661 cttcgcgaac gaggtgaggg cgcagaccgg gaaggccctc gcaccgggcc acgcgctcgt
    15721 cctgcagacg ctcgcccgga cgctctgagg agccggactc gtcacccgcg gtcgcgtaga
    15781 ggacgaggac ggcacggcgc gccgggagga aacatggact tcgatctcga ttcggcggcg
    15841 gccgtcctcg agcggactcc cggcacgctc cgggcccttc tcggcggcct gtccgacgga
    15901 tgggtgcgcg gcacggaggg cccggacacc ttcaacgcct tcgacgtcgt ggggcacctc
    15961 atcgacgggg aggagacgga ctggattccg cgcgcccgga tcatcctggc ccgtggcgag
    16021 gacccccgct tcgtgccgta cgaccgcttc cgccaccgtg cgcgcaacgc cggccggccg
    16081 ctcgcgtcgc ttctcgacga attcggttcg ctgcggcggg cgaacatggc gctgctcgcg
    16141 tcctggcgtc tgacggcggc cgacctcgat ctccccggca cgcatccgga cttcggccgg
    16201 gtcgccctgc gcgagctgct cgcggcttgg gtcgtgcacg acctcgggca cgtcgcgcag
    16261 gtggcgcgcg tgatggcgaa gcagtacggc ggccaggtgg ggccgtgggg cgctttcctg
    16321 ccggtgctca ccgaccgccc ggtgccgcgg tccgggggga gcgccccggt ctcctgagtg
    16381 cggacggccg gtcacgcacc tcgtcgagag cgccggcggg ggaacgtcgg gaacctcgtc
    16441 accgtccctg attcggggtg ggagtgggag ccggcgtcgg attcggcggt gcggtcgtca
    16501 ggaggccggc cttcgggatc agtccgggat tcaggacttt cgcccgtcgg attccggcgt
    16561 cgacgttcgt gatgctgatg acgttcgagg cgatgttgat gannnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnnnn nngtcccaga cgaagttgcc gatgttgacc ggcgtgggcg
    16741 tgcgcgtggg cgtgggcgtt cggaacacgc ggattccggc gtcgacgttc gtgatgctga
    16801 tgacgttcga ggcgatgttg atgacgtcgg tgaagccgag gtcggagccg gaggggttga
    16861 cgtcgctgtc cttctggtcg tcgccgccgg cgttcttcgt gctgaaggtg tcgctggcgt
    16921 gggggaggac gacgcggacg cggtacgagc cgggctgggg cgcgacgacg atgtagttgc
    16981 ccgagccgtt cgtgacggcc gaggcgatca tctgcgactt ggcggggttc cagagctgga
    17041 cggtgacgcc cgcgaggccg ggctcgccgg cgtcctggac gccgtcgccg tcgaggtcgt
    17101 cccagacgaa gttgccgatg ttgaccggcg tgggcgtgcg cgtgggcgtg ggctgcgcca
    17161 ccacggcggc cttgtcgagg acgccgggcc ttggagccgg ctgcgcgaac gcggcctgcg
    17221 gcgcgaggca gagacagagg aggccggtgg tcgctctcat gcgttgattc gtggtctcaa
    17281 tgcattctag cgaaaggggg gaggccgccg gcctccccct cgacgctccc cgacggcgcc
    17341 cgcctctacc agatgctcgg ccggtcggcc tccgcacggc gcatgggcgt cccctccgcg
    17401 cacccgaacg cgtcgtagaa ctccggcagg ttcgacggcg gcccgagggc gcgccacttg
    17461 ccggggctgt gcgggttcgt gttgagctgc acgcgcatcg cctcgtcgcg gtactggctg
    17521 cgccagatcg tcgcgtacga caggaagaag cgctgctcgg gcgtgaagcc gtcgatcttg
    17581 gcgttcttcg cctccggctt gccggcgtag gccttctgca tggccgcgta ggcgatcttg
    17641 atcccgccga ggtcgccgat gttctcgccg agcgtcagct tgccgttgat ggactggtcc
    17701 ggcagcggcc tgtaggcgtc gaactgcttc acgatgagcg ccgtccgcgc gtcgtaggcc
    17761 ttgcggtcgt cggcggtcca ccagttcttg aggttgccgt ccgcgtcgta ctggctgccc
    17821 gagtcgtcga agccgtgcga catctcgtgc ccgatgacga cgccgatacc gccgtagttc
    17881 acggcgtcgt ccgcgtcctc gtagaagaac ggcggctgca ggattccggc ggggaagacg
    17941 atttcgttca gggacgcgct gtagtacgcg ttcaccgtgg gaggcgtcat gccccactcc
    18001 gtccggtcga tcggcttgcc gagcttcgcg aggttccgct tcgtctcgaa gacccgagcg
    18061 cggcggacgt tctcggcgaa tgggacgtcg cgggagatcg tcagggccga gtagtccttc
    18121 cagacgtccg gatagccgat cttcacgttg aaggcggcca atttcctctg ggcggcggcc
    18181 ttcgtctcgg cgcccatcca cggcagcgcc tcgatgcgct ccttgagcgc cgcgcgcatg
    18241 ttctcgacga gcacccgcat gcgctccttc gccttcgggc tgaaggcgcg cgcgacgtac
    18301 atcggcccga cggcctcgcc gagggccatg tccgtcgccg cctgcacgcg tctccacgtc
    18361 ggctcctgct ccggggtgcc gttcagcttc ttcccgctga atgcgaagtc ctcgtcctgg
    18421 aacgccttcg agaggaacgg cgcagccgcg cgtgtggcgt gccagcggag gtacgtgcgc
    18481 cagtcggccg gcgggacgga gcgcgcgtag gccgcgaagc cgacgaagaa cccgggctgg
    18541 cggacgttga cctcggtgga ggcggaggcg cccatctcgg cgaggaactt cgcgaagtcg
    18601 aagcccggag cctcggcgtt cagggcggcg agcgtcttct tgttgtaagt cttctgcggg
    18661 tcgcggttct cgacgcgcgt gatcgaaact ttcgccagct ttgtttcaat gtccatcacg
    18721 acatcggctt cggccttcgc cgcctccggc gcgtcgccca cgagctcgag caccttcgcg
    18781 acgtgcgcgc ggtaggcctc gcgcaggtcc ttcgacttgg cgtcgtcctt gaggtagtag
    18841 tcccggtcgg gcattccgag gccgccctgg ttgaagatcc cgatggtacg cgtggagttc
    18901 cgcgcgtccg gcgcgacgcg gaagccgaaa cccgcgttcg ctcccgccag gtgcagctcg
    18961 gcgaggacgg cgggcaggtc gtcgggcgac ttcagcttcg cgatcgtcgc gaaggacggc
    19021 gcgagcgggg cggcgccggc cttctcgcgc gccgcggcgt ccatgccggt cgcgtagaag
    19081 tcggagacct tctgctgcgg cgtgcccttc ggccagtcgg ccttcccgga cgtctcctcg
    19141 aggatcgact tcacgacgtc gcggttgcgg tccgcgagcg cgtcgaagcc gccgtagcgg
    19201 ctgcggtcgg ccgggatcgg gtgcgcggcg agccacgcgc cgttcgcgaa ctggtagaag
    19261 tcctcgcagg gcttcgcaga cgcgtcgaag tcggcggggt cgatcgcctt cggcttcgcg
    19321 gaaggagcct gggcgaagag ggagtgtccg gcgaatgaaa aggcgagaac aaggaccgtg
    19381 cgaaaacgca ttgcgtctcc tttgggcccg agtctagagg gagagcgggg tcaccgccct
    19441 cccgccactt gcatcaggcg acggcggcct gccgggaatc ccgggctctc cgcaggagcg
    19501 ccacgatgcc gccgagcatc atcacgtaga agccggccca gacgagggag atgaggggct
    19561 tcgtcgtgac gtcgatcgag agcgtctcgg ccgtcgccgg cttgaggtcc ccgcccgcgg
    19621 cgacgcccat gagctccagc tcgcacgtcc cctcgttcgg ggagacgcgc ttgatgcgca
    19681 tccggcccct gccggggagc ggggtgtcgg gcgcctcggt ggccgggccg ccgtcggtcg
    19741 cgccgaagta catgacgaac ttcgccgtct tctcttccgc gacctcggcg gtcgtcacga
    19801 ggaagttcgc ggtgacggtg acgtgcgggc gcttcgcgtc gttgatcgcc gagcggtccg
    19861 ccgagaagtc caggaacttg atcttgacgc cgtcggcgct cnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn ngtcttcgtc tcgtagacgc cggagacgag gatcccgacg
    20041 agcatgatcg aaacgcccac gtgtgcgaga tagccgccca cggtggaaac cgccttgcgc
    20101 cgcacgaaga gcgcgaccgt ctcggcgttc gcggccatcc cgaagaccgc gaaggcgatg
    20161 atcgcgaggt cgcgcgccga gtggacgccg gccgcgaagg ccactccgcc gcccatgagg
    20221 ccgatggcgg cggcgacgaa cgccttcttc gccacgacgg cgagagtctc cccgcgccag
    20281 gacaggtacg ggacgagcgc gacgaggagc gccatgagcg ccgcgatcgg cgcgtgcgtg
    20341 acgttgtaga agctcgtcga gacctgcgac gccttgccgc cgatgcgcgt gaggatcggc
    20401 gcggacgttc cgaggaggat gacgaggccc gaggcgctga acagcgtcac ggagaggatg
    20461 aagagcaccg agcgcgagaa gaagggttct tccttctcct tcccggtctc cgcgtcgatg
    20521 tccgagacga ccggaatctg cttccagcgg aaggcgagga ggccgagacc cccgaacagg
    20581 aagaccacga tgatggcgac gagccagccc gtgatcccga ggtcgatgaa ggagtggacc
    20641 gagaagtccg cgaggacgcc ggagcgcgtc aggaacgtgc cgtagaggat gcagcagaag
    20701 gcgaggatcg cgaggatgat gttgatcttc cggtgtttct cgcgcgcctt ctggaggtac
    20761 atcccgtgga cgagggcgac ggtcgcgagc cacggcacga gggacgtgtt ctcgacgggg
    20821 tcccacgccc agtagccgcc ccagccgagc gtcttgtagg cccagtaacc gcccatgaga
    20881 atggcggttc cgagcgtcac gaacgtgaag agcgcccagg gcatcgcgcg gacgacccag
    20941 ccgtcccagc gcttcatcca gagcgccgcg atcgcgaacg cgaacggcac gctgagggac
    21001 gcgaagccgg agaacatcac gggcgggtgg atgaccatcc acgggtcctg caggagcggg
    21061 ttcaggccct ggccgtcggg cgggatctgc ccgaggaagc ggaacggcga ctgcttcacg
    21121 aggatcgcga cgatcccgat gaaggaggcg acgtacacga tcatgaccgg cggctcctgc
    21181 tccttggccg aacgaaggac gaagaggccg atgagcgagc cgaaaaacag ccagaggagg
    21241 aacgagccct cctgcccggc ccagaacgtc gagaacaggt agtagacggg gaggtcccgg
    21301 cccgagtagg aggcgacgta cgagacgcgg aagtcgtggg acagaagccg tcccatgagg
    21361 aggaccgagg cgaggacgac cgagaatgtg aaggcgtagt acagacgccg ggcgaggaga
    21421 agatccggat tttcttcgaa tggtagtagg gcagggctcg cggcgactgc tgcggcagag
    21481 ccggagccgc tcttaccctt tctaagaaaa tcttctgcgg catggcccat gcgcatgact
    21541 cgccagtagg cgagcgcggt caggacgccg aagacgagcg ccgcccacat cgcgacggtg
    21601 cccggcgcga aaccgagggg agatcccagg cccatgtcag gccttcttcc cgtactgctt
    21661 ctcgatctcc tcgccctggt acttcgaggg gcatttcacg aggagcttct ccgccgtgaa
    21721 tcggtcgttc tcgtagcggc cgatcgcgac gatcgacacc gcgtcctcga agttcgccgg
    21781 cttgacgtcc gtgtaggaga cggggatcac gtccttcgtc tcggggtcga cgagcgtgaa
    21841 gttgaggacc ttcttgtcct ggtcgtattt cgtggagccc ttctcgaggg cgcccatgac
    21901 ctggaccgtg cggtgagagg tctttgcctc ggagaagctc acgtacggcg tcatggactt
    21961 cgtgaacgtc gtgagtccga gcgcgaggaa gccgaggatg aggacggctc cggcccaata
    22021 agcgcgtttc atggcttgtt ctccccgccg gagcgcgtct ctgcggcgcg cacccggcga
    22081 tccaggtaga cgaggtagcc gaagattccg gcccagatga cgatgttcac cgccgcgacg
    22141 tagccgagag ccgtgctcat gccgacctct cgcggacgat gcgggaaatg cggcggtcga
    22201 ggttccacat ccaggtgaag agtcccatga aagccaccat cgacgagaag aagatgaggc
    22261 ggatgtcgga cgacatcttg accttcccct cggtgttgat cacggtgtcg gggtgcagcg
    22321 actggtacat ccgcggcacg gcgaagacga ggaacggcac cgtcacgacc gcgacgagcg
    22381 cgtaggccgc cgagagcgcc gcgcgccgct cggggtcctc gagggcgccc ctcagcccca
    22441 ggtaggcgcc gtagacgagg agaaggagga cgatcgacgt ctcgcgcggg tcccagttcc
    22501 agtaggagtt ccacatgacg cgggcgaaga tcgagcccgt cacggtcgcg aggacgcaga
    22561 agagaaaccc caggcgcgca gcggccgacg cggcgtcgtc gtcctcgatc cggcgcttcc
    22621 ggaggtacct ccacgaatac caggccgcga cgaagaaggc gacggtcgaa acccaggcct
    22681 gcgggacgtg gaagaagacg attcgcgagg actcgccgac gaagttctgg gccggacgcg
    22741 cgtagaggaa cgccccccag cagatcgccg ccatcccgag gcccaggaac cacttggaga
    22801 tgagtccgag actcttcacg ggcggatcat accttccgga ccggggcgcc ccattggtgc
    22861 atttgcttca gagactcgga ctcagaaacg gtaggtcagg cccaggagcc agcggttctg
    22921 cgtcgccgtg tcgtcgtcca gttgctcccc cgtcagcagg agcgagaacg cgcgggacat
    22981 gttccagatt cccgaaaggc tgtagcgcct cgaggagggc cggttcacga tcgtgaggcc
    23041 gcccgagtcc gtgatctgca ggacggacag cgacgtggag tagtcgaggg tgaggtagaa
    23101 cttcgacccg aggctccttc ccacggagaa gtaccaggcg tcgtagctgt tgctgcccgg
    23161 gcggttcgtg cggttgtccg acacgaacac gtcgaagccg gtcccggcga cgttcatcgc
    23221 ggacagcccg aactgaaggc ggttctgcgc gacgtcgtcg cggttgctct tgtcctggta
    23281 gtagcccgcc caggcgcgcc atccgcgcgc gaactcgacg gtcgcgcggc cgcccatcga
    23341 atcgaagagg aagccgtcga ggctcttggc gtcgatcggc cgtcccgcga tctggtcctg
    23401 cgtgatcgaa cgcgcgtcga ccgagcggcc gtgctgatag agggcctgga tctcgaagga
    23461 ccggaccggg gaccaccggg ccgtcgcgaa gaagtaggcg aggccgccgc tccccgctcc
    23521 tccgggcccg gtcaggtcgt attcggccgc ctgatagagg aagaactccc ggccgatcgg
    23581 gatgaagttc gtcgtcgtga ggacgttccg ctccgcgagg ttctggttgc ggatcgtcac
    23641 gaagccgagg acgtcccgcc agccgcgctc gccgtcgtac gcgacgtagg cgccgccttt
    23701 cttgacgtcc tggacgtagc ccgggtcgta gcccttcggc tcgaggccgc cgaagacgcc
    23761 gacccggatc ttcccggccc gcgcctcgag agagagaccg cccacaccgc cgagggctcc
    23821 gagatcgttc aggtacatct gcccgaggcg gacggccacc ttgccgccgg cggcgcggcc
    23881 gccgacccac gcgtcgtaga cggtccagcg ggggtcgcgg ttcgtgacgg acgggtactc
    23941 ggagccgcgg gcttcgaggg agaactcgaa gccgtccacg tcccgcggcg gcgtgtgaat
    24001 cgagaccgac ccgctgagct cgttgtagtc gctcgtcgtc ccgtcgacgt tcttcgtgcg
    24061 gatgacctgc ccgaacacgg agatgcgtcc ccagtcgagc gccgactgtg ccgcggccgg
    24121 ggcgccccat gcggcaaggg ccgcgagaat ggcgagacct cggcgcatca gctcttcccg
    24181 ttcgggtggc acgagtagca cgccgtcgac gtccacgaat agcccgcgcg gcccttgtgc
    24241 ttggagtcgg tcgtcgcttt cgtgtggcag ccgatgcagt tgaagaccgt gaacacgttg
    24301 atggtcgtgt ggcactggtt gcagggaacg ttgtggttcg agacgagcgg gaagtaggtg
    24361 tggttgaact tgccctgctg ccacgtcgcg tcggtcgcct tgtggcacgt gtcgcacgag
    24421 gtcgggaacc cggccgcggt gtgactgggg ttcttcgaat tgttgtagtc ggtctggtgg
    24481 cagccgacgc acgtggtcgg cgcgctgcgg tacggcatcg tggtgtggca gcgcgcgcag
    24541 gtctgcgtcg cgtgaactcc cacgagcggg aacggcgtca tcgtcgaatg gttgaagatc
    24601 gaaggcgacc aggccggcgc cgagaactgg tggcaggtgt cgcaggtcgt cgagaagccg
    24661 cccgccaggt gcggcgggtt caccgagttg ttgtagttct tcgggtagtt gttcaggtgg
    24721 cagccggagc acgtcgtctg cgcgttgcgg tagggcgtcg tcgtgtggca ggtcgcgcaa
    24781 gcctgggtca ggtggacacc ggcgagcggg aagggcgtgt tcgtcgagtg gttgaacgtc
    24841 gagggtttcc aggccggcgc cgagaactgg tggcaggtgt cgcaggtcgt gacgaacccg
    24901 cccgcgaggt gaggcggatt gacggacttc tggtagtcgg cctggtggca cgtgtagcag
    24961 tcgcgggccg tgcccttgta gacgttgttc ttgtggcagg cggtgcaggc ctgggtcgcg
    25021 tggacgccgg cgagcgggaa cgtcgtgttc gtcgagtggt tgaagctcgc gggcttccag
    25081 gccggcgccg agaactggtg gcaggtgtcg cacgccgtcg ggaagcccga ggtcacgtgg
    25141 ggcggggtca cggacttctg gtagtcggcc aggtggcacg tgtagcagtc gcgggccgtg
    25201 cccttgtaga cgttgttctt gtggcaggtg gcgcaggcct gcgtcacgtg gacgccggcg
    25261 agcgggaacg tcgtgttcgt gttgtggttg aagctcgcgg gcttccaggc cggcgccgcg
    25321 aactggtggc aactgtcgca ggtcgtcgga aagcccgcgg aggcgtgcgg cgggttgacg
    25381 gccttctggt aatcggtcag gtggcatgtc ccgcacaggc gcgacgtgcc cttgtagacg
    25441 ttgttcttgt ggcaggtcgt gcagggcgtg ttcaggtgga cgccggcgag cggccatccc
    25501 gtcatggact gtccggccgc cggtcctccc caggcgagga gagcggccag gaccgcgacg
    25561 aggtggatct cgtggaggtt catgccgagc gggaacagcc tggtgtttct cacgctacac
    25621 actcccggtt gaggactcat tgcgtttcag tgtcggaaga tcgacggcac ccaggccggc
    25681 gcagaaaaca ggtggcaggt ctcgcagttc gtcgagaagc ccgcggcgag gtgaggcgga
    25741 ttgacggatt tctggtagtc gacgacgtgg caggagtagc agtcgcgcgc ggtgcccttg
    25801 tagacgttgt tcttgtggca ggcgctgcag agctgcgtcg tgtggacgcc cgcgagcggg
    25861 aacgtcgtgg acgtggagtg attgaacgag gagggcttcc aggccggcgc ggagaactgg
    25921 tggcacgtgt cgcacgtcgt cgggaaaccc gacgaggcgt gcgggggatt gaccgacttc
    25981 tggtagtcgg gctggtggca cgtccagcag tcgcgggccg tgcccttgaa gacgttggcc
    26041 ttgtggcagg cgctgcagag ctgcgtcacg tgcgtgccag cgagcgggaa ggtcgtggac
    26101 gtggagtgat tgaaggtgga aggcttccag gccggcgcgg agaactggtg gcaggtgtcg
    26161 caggtcgtcg ggaaccccgc ggcgacgtgc cccggattcg ccgagttctg gtactcggtc
    26221 ttgtggcagg tgaagcagtc gcgggccgtc cccttgaaga cgttggcctt gtggcaggcg
    26281 ctgcagagct gcgtcacgtg cgtgccagcg agcgggaagg tcgtggacgt ggagtgattg
    26341 aaggtggaag gcttccaggc cggcgcggag aactggtggc aggtgtcgca ggtcgtcggg
    26401 aaccccgcgg ccacgtggcc cggattcacg gagttctgga agtcggtctt gtggcaggag
    26461 tagcagtcgc gcggcgtgcc cttgaagacg ttgttgacgt ggcaggtcgc gcaggtctgc
    26521 gtcgcatgga cgcccgcgag ggtgaacgtc gtcgccgtgt tgtggttgaa gctcgcgggc
    26581 ctccacgccg ggtcggagaa cttgtggcac gtgtcgcagg tggtcgcgaa gccggaggcg
    26641 acgtgcggcg ggaccttcga gttctggaag tcggtcttgt ggcaggagta gcagtcgcgc
    26701 ggcgtgccct tgaagacgtt gttgacgtgg caggtcgcgc aggtctgcgt cgcatggacg
    26761 cccgcgaggg caaacgtcgt cgccgtgttg tggttgaagc tcgcgggctt ccacgccggg
    26821 tccgtgtact tgtggcacgt gtcgcaggtc gtcgcgaaag cggccgccgt gtgcgacggg
    26881 tttctggagt tctggtagtc ggccttgtgg caggagtagc agtcgcgcgg cgtgcccttg
    26941 aacacgttgt tcacgtggca ggccgtgcac ggggccgtca cgtggacgcc ggcgagggtg
    27001 aacgtcgtcg tcacgttgtg gttgaacccg gcgtccttcc agtttggagc cgagaacttg
    27061 tggcatgagt cgcaggcggt cgggaacgcc gcggcggcgt gcggcgggtt cttcgagttc
    27121 tggtaatccg tccggtggca cgtgaagcag tcgcgcggcg tgcccttgaa gacgttgttc
    27181 ctgtggcacg cggcgcaggg ctgcgtcacg tggactcccg ccagcgggaa cgtcgcgtgg
    27241 gtgaagttcg cgccccgcca cgtcggcgac gaggccttgt ggcagcccgt gcagtccgtc
    27301 gggaacccgc ctgcgacgtg gcccgggttc tggacgcgct cgtagtccgt gcgatggcag
    27361 gaataacagt cacgagacgt ccccttgaag acctgggcct tgtggcacga ctcgcagtcg
    27421 agcgtccggt ggggcgtctc gagggtgaac gtcgtggcgg ccgcgtggct ccacgtgacg
    27481 gcgctccatg tgatgggccg gtggcaggtc tggcagtcgc cgcccagcgc ggtcttgaat
    27541 cggtcgtcct gccttcggac ccagtggcag tcgtaacagc ccgtcggggt acccttcagg
    27601 acccctttga tgtggcacga ggcgcacggg accgcaaggt gcttgccctc gagcgtgaag
    27661 gcggccgtct tgtggaaggc gcgcgaggcg gtccgccaga tgacggcgtt gtggcaggaa
    27721 gcgcagtcgc gcccgagggc gccgcgatgc acgtcctcgt gacagtggac gcacgcgacc
    27781 gggaccgcgt acctcacggc ggtcccccgc ccggcggggt agtccgccgt ctccctcttg
    27841 tggcactcgg cgcacgcgat cgtcgcgtgt ttggcgacga agaactcctc ggtctccttc
    27901 ttcttcgggt gcttgaacgc cgccaccttg aaggacccgg cgtcgtgaca cgtctcgcaa
    27961 cgcggcgaga gctggccgag gtgcggatcc ttgtggcagg cccggcactc ggtcgagagc
    28021 ccggtcaggc gcacggccgt tccattgccc gaggggaact tcccggtctc cttcgcgtgg
    28081 cacttcgcgc agggaacggg ttcgtgcttg cccttgagcg cgaacttcga gttcgtgtgg
    28141 acgaagagat ccgccttgta acccttcacc gcgagcccgt ggcacttcgc gcagtccggg
    28201 ccgacctggc cgaggtgcgg gtccttgtgg caggccgcgc acgcgaacgg aacgcccttg
    28261 tactggcggg atccggccgg cggtccgatc cccttgacga ggcccacgac ggacgtcgcc
    28321 tcgggaagat ggcacttctc gcacgcgatg ccggcgtgcg caccctggaa gaactcgggc
    28381 tggcgcgggt gctggaaggc cgagagcttg aaggaagtcg tcccgtggca cgtctggcag
    28441 gtcgtcccga gctttccctt gtggacgtcc gcgtggcacg aagagcatgc cgcgttcgcg
    28501 ccccggaaat cgacgattcg ctgcgagacc ggcatgttga cgcgggcctg gacgccggcg
    28561 gccttgtggc acgcgacaca agcggtcgtt gcgtgcttcc cgtcgagcgc gaacttcgtc
    28621 cgggcgttgt ggtcgaactt cgcgggcttg aaccccgcct ccgtatggca cgccgcgcag
    28681 tcctccgccg ggaagaggcc cttgtgcacg tccgtgtggc agtccatgca gcgggcgtac
    28741 ttgaggcgga cgacggtcgt cggcttgacg tggcaggtct ggcaggcgac ggcagcgtgc
    28801 cggcccttga gcgggtagcc ggtcttcgcg tggtcgaact tctggccttc ggcgagcgtc
    28861 ttgaagctcg tcgtcacgtg gcacgtcgtg caggtcccga gcgcggggac gtgcgggttc
    28921 ttgtggcagc cctcgcaggc gccgaagcgc tcgatccggt actggccctt cgtcttgtgg
    28981 cagctctcgc acggggcctt ggcgtgcgcc ccggtcagcg ggtacttcgt cctcgaatga
    29041 tcgaagccct tcttcgtgtc cttgaaggcc acgttcgtgg ggtggcacgt ctggcaggtg
    29101 gcgccgaggg tcccgttgtg gacgtccttg tggcacgacg cgcacgacgg ggacacgctg
    29161 aggtaggagc gcgtcttgtg gcacgccttg cactcgagcg gcgcgtgcag gccgtcgaga
    29221 gcgaacccgg cctcgccctt gtggtcgaag cccttcggag tgaagtggag gagatcgccg
    29281 tcgatgccgg catgctcgac gtggcagccc gcacagtcct tgcccgacgc ctcccggtgc
    29341 acgcccttct tcgccttgat ccgttcggcg acgggcttgt ggcattcgag acaccgggcg
    29401 gcggtgacct ggcggcccgg ttcgtggcag ctctggcact tgtcgaggcc ctcgagcttt
    29461 gtgtgcggcc tggaaagctt cccgggcgag acgaggctgc cgagcccctg cgcgccggca
    29521 ggcgaggaga atgcggcgag cgcggagact gccagcccga gtccgagccg cgaggcccgg
    29581 tgacctcgcc cgcgccgtcg cgtgtacgcc ggttcgtccg ctttcctcgc ctgcatcgtt
    29641 gccgccgtca cgatggcctc gactgctttg gcgtgccgtc gtcctcgaag tgctcccgat
    29701 accagctttg gtagtggtgc ctcgcccatt cgcggtcgac acggcccgag atcattccgg
    29761 tgagaccgac ccgggtgctg gggttgacga ccgccatgta gatgtgaccg aacatcagga
    29821 gcggagccac cagggacatg ctgatgtgaa gaagccaggg caggaaatgg tctacgcccg
    29881 tggtccagag aaacaggccg gttgcgacaa acaggggata cgtgaccatc accatcatga
    29941 agttcagccg ctcggccgcg ttgaacttgc gctgctccgg aaggacgatc cgcttactga
    30001 cggtcgaagg cccgaatagg aagagccact tgagatcgtc gatcgtccag gagaacccga
    30061 tcttgatgtt gtacaggtgc ttcttgtagt cgcgccgggt gtgaaggatg accagtgccg
    30121 ggaagacggc caggcacccg cccccgaccc gatggaggaa caggaggacc tcccggacga
    30181 ggctgtctga gtggaggttg tagccgaact tcacgatcac tcccgtcagg aagcaggtca
    30241 tgaacgggag cgcgatcgac cagtggagga gcatctcccc tctgacgaag cggaggatct
    30301 cccttgcgtg ctcctttcgc ggaaagacca ggacctgccc cgggggggac ggcgggtccg
    30361 ggtgcgccgc ggagggcgcc ttcgccggcg acgcgaggag cgtcgtcggt gaggcgaaaa
    30421 ccctcgcgtg gcccgggacg ctgcccgccg cctggtccaa tcccctctac ccccttcagg
    30481 aaatgccggt ttgtcaccgg ggtgcacggg ttttccgaac aggtcccgca aaaagcgaag
    30541 ctatgccctc aagtctgact cgggctttcc tgagagtgat tccacctaac gttgagagag
    30601 agtctcaggc cgcgggtcgc cttcgaaaag gcaggaagcc ggaggtgcgg gcctattccc
    30661 gccagacggc gtcgaaaagg gcgaaggccg ccgtcgtgac gaccccgtcg taggcgacga
    30721 gcacccgcac gaagtcgagg ccggccgaga agtccgccgc ccgggcggcc acctgcgttc
    30781 cggagaccgc ggcgatgagc ggcggaagga ggagcggaat ggagaggacg gcgaagagcg
    30841 ctcccttggc cgccgccttc gagacgagcg ccgcggtgaa cgtcgacgcg cccgcgaggc
    30901 cgagtccccc gaggacgagc aggaccacga agagcgtcgg gcacgcgatc tcgaaggatg
    30961 tcaggacgag gaagagcggc acgatgaagg ccgtcaccgc gaagagcagg acgatgttca
    31021 cgaggtactt gccgaggaga accgcggccg gcggcgccgc gagccgaagg gcggcggccg
    31081 ttccgccctc ctcttccttc acgaacacgc gggcgaggcc cgtcatcgcc gagaagaaga
    31141 tgacgatcca caggagcacg gcgttcacgg tggggcggtc ctccggagcg aggcgcgtcg
    31201 ggccgaccgc gtagccgacg aggacgagga cggcgaacgc gaagaggaag agcgcgttca
    31261 ccgcgatgcg cgtccgccac tcggcgagaa ggtccttgcg gaggatggcg agagcggccc
    31321 cgagaagggg ggcgggcgag cgggcgttca caacgacacg gtctcgtcgg cgagcgcgag
    31381 gtcgcgcggg tcgttcgacg cgatgatcgc gacccctcgg gatcgctggg aggcgacgac
    31441 gcgggcgacg acttcgacgc cgtccgcgtc gaggttcgag gacggctcgt cgaggaagag
    31501 gacggcggga tcgtggagcg tcgcgtacgc gagcttgacg cgctggcgct ggcccgtgga
    31561 aagagaagag agagatttct tcgagtgtga atgttcggaa aggcctacgc cctcgaggag
    31621 cgcacgcgcg cggccaggcg atgccggaag gccgtggacg ctttggaaga acgtcaggtt
    31681 ctcctcggcc gtcagctcgc cgtagagcgc gaggtcgggc gccacccacc cgatgcacca
    31741 gggaacgtca cgcggctctt ggccggcccc tgtgctttcc ctttctaaga aaatcttcgt
    31801 cgacccaccg ctggggcgta tcaggccgga gagggtcttc agcaaagtcg tctttccgct
    31861 cccgttcgaa cccgcgacgc cgaggacgcg cccctccggg acctcgaacg acagcggtcc
    31921 gaagatcctc cgggcgccat acgagcgcgt caggccatcg acaacgagcc gcatgcgggc
    31981 ggaaggataa tccctcccgt gctgcagcga ttcctcggcg tcctcgtctt cctcgcgatc
    32041 tcgctctgga aggcgacgct gcgcatcacg atcgtcggcg aggagcaccg gcaggcgatc
    32101 cgggcgaagg gcaagaagcc gctccacgcg atctggcacc agcggatggt gggcgggatc
    32161 ctcgcgcacc gcggcgaggg ctacgtcacg atggcctcga agagcgagga cggcgagatc
    32221 atcgccacgt tcctcaagta ctggggcttc gtcgccgcgc gcggctcgtc ctcgcggggc
    32281 ggtgacgtcg cctccgccga gttcctcgag gccctgaagg tggctcccgg cggggcgctc
    32341 acgcccgacg gcccgcgcgg gcccgcgcgg acgtgcaagc gcggcatcct cgtcctcgcg
    32401 gagcagggga acggcctggt cctgccgtcg gcctcgtcct cgtcgcgccc ccgtttcctg
    32461 aactcgtggg accgcttcct cctgcccatg ccgttctcgc ggtgcgtcgt cgtcttcggg
    32521 ccgccgctcg agcgcgcggc ggacgaagcg gaaggggcgt tcctcgcgcg cgccgcctcc
    32581 gcgatcgacg ccgccaccga cgaggcggac cggctctgcg gcgtcgtggg cgcgccgcgc
    32641 gaaaggatga agagggcatg acgctccccg tcacagtcgc ggacgtcgag gccgcccggc
    32701 gccggatcgc gccccacgtc caccgcacgc cggtcctcgg ctcctcgagc ctcgacgcgc
    32761 gcgtcggcgc gcgcctcttc ttcaagtgcg agacgttcca gaaggtcggc gccttcaagg
    32821 cgcgcggcgc gttctcgcgg ctgactcttc tgtccccgga agagcggcgc cgcggcgtgg
    32881 tggccttctc ttccggcaac cacgcgcagg cggtcgcgct cgcggcgcgg acgctcggca
    32941 tctccgcgac gatcgtcatg ccgcacgacg cgcccgcgct caagctcgcc gcgacgcgcg
    33001 gctacggcgc ggcggtgaag gtgtacgacc gcgcgcggga gagccgcgag gcgatcgcga
    33061 aggacatcgt cgagcgcgag gggcgcgtcc tcgttccgcc cttcgacgac cccgccgtga
    33121 ttgcggggca gggcacggcg gccctcgagc tcctcgagga cgttccggac ctcgatctcg
    33181 tgctcgcccc ctgcggcggc ggcggactcc tctcgggcgt cgcggtcgcc gcaacggcgc
    33241 tccggccggg gatcggcatc tggggcgtcg agcccgaagc cggcgacgac ttccggcgct
    33301 cgctcgaggc gggacatccc gtcgagatcc ccgtccccgc gacgatcgcg gattgtctcc
    33361 agacgacgcg cgccggagtc cacacgttcg cggtcgtctc ggcgctcgcg aaggggatcc
    33421 tgaccgtgag cgacctcgac ctgcgcgtcg cgatgtccgt cctcttcacg cgcatgaagc
    33481 tcgtcgtcga ggcaggcgga acggccgggt tcgcggcgct tctcgccggg aaggtcccgg
    33541 acgcggcggg caggcgcgtc ggggtcgtcc tttcgggggg gaacgtggac cccgagcagt
    33601 tcgggaggct cttgacgggt ctgcccgagg gctcggtcta ggcacgccga gcggaaggtg
    33661 cgggaacccg catgacgggt tcccgccttc agaacgcgac gttcacgccc gcggtgacgc
    33721 ggagcgtgtc ggacttcagg cccaggtcct tgaccgccgc gacgtcgaga aacgtccagc
    33781 ggccgtcgat ccagaacgag agcttctcgc ccgcgcgcac gtcgacgccc gcgccggcat
    33841 ggagcgcgaa gaggttctgg gacgagtacg cggtcccggc cgggggattc ccgacgtacg
    33901 ggttcaccca gtggtacccg atcccggcga gaaggtacgg ctgcacgcgg cggctgaaga
    33961 agaagaagaa gaggagcgag ccctcgacgg gcacgtggtc cgcctcgacg gtctcgcccg
    34021 cgcccggcca gctcaaacgc tcgtatccga ccgagacttc caggccgagg gcgcccgtca
    34081 ggcgcggccg ccagtgcagg cctgcggagg ggtgcgttcc gtcgacgtcc gcccccgcca
    34141 cgacgccgcc gtgaacgccc cattgcgcgt ccgggtccga gttcgactgc gcggcaccgg
    34201 gggcggcggc gaggaacgcc gccgcggcac aggcaagaac gatcggacgg cgcggcatcc
    34261 ggtgcggact ctaccgcgcc cggaccccgg atcgctcata atcacgatac ctgtggccgg
    34321 cagaacctgg aaggacgacc tcccgtcgct gattctcgcg ctcgcggcgc agctcgggct
    34381 ggtccgcgcg gtggattggg cgcgcgcgaa cacggccacg cacgatctgg cgcggtcgct
    34441 ggtcgagaac agattctatt tcgcgccgca gatcggctcg ctcctcgtcg tcttcctctt
    34501 cgcgcggctc gtcgtgaacg ccctgaacct gcggggcgtc gccatcggcg actggctgcg
    34561 ttcctcggcc ctgctcctcg tgctcggtct cgggtgcctc gtggccgccc acctcgacca
    34621 gatcctcgcc ctcgccgcgg agatcctcgg gaccccggcg ctgcaagccg ccgcccagga
    34681 cgtcgccgcc tcgtcgttct cgcgcgtgct cgggcgcacc gcgacgctcc ggttcgcgat
    34741 cgacctcgcg ggcctcctct gctgcctctt cgcgatccgc ctcgggaaac cgtcgctccc
    34801 cgacgcccag ctcgcccgcg gatacgcgtc ggcgaaagac tgggtccggg cgggcgacgc
    34861 gtacctgaag gccggcgacg tgcgccgcgc gcgccgcatg ttcaagaagg gccggtcgtg
    34921 ggcgcgcctc gcggcgctcg agatgcgcga cggcaatccg gccgaggccg ctcgcctcta
    34981 ccaggatgcg ggagacgcct ggctctggga ggcctcgcgc gcctggctgg cggcggacaa
    35041 cccggcggcc gcggaggaga cggggctcaa ggcgctccag ctcgcgcgca cgggcggccg
    35101 ctgggaccgg ctcgcggaga tcgcggaggc ccttggagac aacggcactc tcgcggaggc
    35161 gtgccgcaag ctcgccgagg cgacgacgag cggtccggtt cgttcggcgc tctggaagcg
    35221 cgcgggcgag gcgtaccgcg tggccggacg ccccggcgag gcggccgagg cgttccgcgc
    35281 cgcgcaggaa tacctgctcg ccggagagct gttcgtggaa agcggccgca cgacggaggc
    35341 cgtccaaagc ttcgagaaga gcggtgcgct gacgaaggcc gcgctcgccg ccgctcgcgc
    35401 gggcgacgag aagtccgctc aggagcttct cgcgcgcgag gccgaggcgc gcggcgacct
    35461 cgtctccgcc gcagacgcgt ggcggcgcgc gggccagccg ctccgggccg ccggcttcta
    35521 cgagcgctcc ggggacttcg tgaaggcggg agacgcctgg aaagacgcgg gccgtccgga
    35581 acgcgcggct gcgctctacc agaagggcgg cgcgaacgtg gcggctgcag aagcctacga
    35641 agcgaccaac cagcacgaga aggcggcgac gctctacgtc gagctcaagc attacgacaa
    35701 ggccgccgcg ctgttccgcg ctgcgggccg cctccccgcg gcggccgctg cactccaggc
    35761 gggccaccag tacgacgagg ccgcacagct cttccaccgg acgggccgga gcctcgaggc
    35821 cgcgcgctgc gcgctcctcg gcgggcaccg cgagaaggcc tgggaatacc tcacgagcgt
    35881 gccgcgcaac gaggaggggc tcggggagct ctttcacgac ctcgcgcgtg cgcacctcgc
    35941 ggcaaacgag ccgaaggacg ccgttcacgt cctgcgcgaa ttcctgccgc gcgggccggt
    36001 cgaggccgac aacctgccgc tgcacatgac gctccttgcg gcgcttcgcg ccgcgggcga
    36061 ctccagcgag ggcgtcgagc tcggccgggt cgccgccttc gacccgtccg tcctcgaggg
    36121 ctcgagcgtg gcgccgcagc ttcctttcgg ggaggtcgaa gtcgacgcct cgatgccgtc
    36181 cgcgctcatc cggacgcccg ccgtgcacaa gtccgggccg tacgcggccg tcacgcgctc
    36241 gccggtcacg cagaaggtcc agccgccgcg gctggcgatc ccgggcccct cacccacgcc
    36301 ggcgcccgct ccggctgcgg cgcagcccgg gcccccgccc ccgttccgca ccggatcggg
    36361 cctccccgag acgccggagg accgctacga gattctcgcg gagctcggca ggggcggcat
    36421 gggcgtcgtc cacaaggccc tcgaccgcaa gctcgaccgt ttcgtcgccg tcaagatcct
    36481 cccgtggcag ctccggggcg acgagacggc gatgcgctac ttcaagcgcg aggccaaggc
    36541 gatcgcggcc ctgaagcacc cgaacatcgt cgccctctac gactacggcg acgggttcgg
    36601 ctcgctctat ctcgcgatgg agtacctcga ggggccgaac ctccagaagc tcatcaagaa
    36661 ggaccccgag cgcgtgcgga agaactggcg caactggttc acccaggcga cgcgaggggt
    36721 cgcggccgcc cacgcgaagg ggattctcca ccgcgatctc aagcccgcga acctcatcct
    36781 cgacgagcac ggcacgctgc gcatcctcga tttcggcctc gccctcccgc aggcggacgc
    36841 gggcggcacg tcgaagctca tcgggagccc cgcgtttttc ccgccggagg tcctgcgcgg
    36901 cgagatgccg agcccggcgt ccgacgtcta ctcgctgggc gccgcgttct acacgctcgc
    36961 gaccggccgc tggccgtacg tcggcgagga cgtcctcgtg gcgcgcctcg agcgcgaccc
    37021 cgacgacccg cggccgtacg cgccgttcct gaacgaggag gagatccgga tcctcatgaa
    37081 gacgctcgcc cggcaccgcc ccgagcgcta
//
LOCUS       DAOMFY010000011        36807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_358_length_36807_cov_8.690547, whole genome shotgun sequence.
ACCESSION   DAOMFY010000011 DAOMFY010000000
VERSION     DAOMFY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 36807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36807
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_358_length_36807_cov_8.690547"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PLB01_02050"
     CDS             complement(<1..1347)
                     /locus_tag="PLB01_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQQ76110.1"
                     /translation="MSYQSALRSLASALAAAALVSLPASAVTLPPGFGDALVANVGAP
                     TALAFTPDGRLLVTTQGGTVRIVSGGTLLPTPALSLGSAVCSNSERGLLGVAVDPQFA
                     TNHYVYLYYTFNKNAAGCPGSNATTPVERISRFTLDVPTANVISPATEVVLLDGVTNF
                     AGNHNGGQLRVGPDGYLYAGTGDGGCDYAGDSGCAGANDASRDRNILNGKIVRIATDG
                     SVPPTNPFLGAGTARCNTGPSAPGTICQETFAWGFRNAFRLAFRPSDGALTVNDVGQG
                     AWEEIDFPIVAGGDYGWPCREGAHAALTSGKCSPTPAGLLDPYFEFPHGTIPGTTTSG
                     CGSITGGAWVPAGAWPASYDGTYLFADFNCGAIARLTPGGPPSASDFATGLGSSTVVD
                     LLFGPSPTGTSLYYTTYAGGGQVRRIDYAVTLPPAATLCSLTPCRVLDTRGADGPAL"
     gene            1522..2835
                     /locus_tag="PLB01_02055"
     CDS             1522..2835
                     /locus_tag="PLB01_02055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ76111.1"
                     /translation="MAVACSYRLDLARTHTVAGIEGLAPWWTYVLKNVDSLDEGRGLV
                     SGSLYAPGQSIASWIVLAVLDGRDDPAVLRIVFGFLDGVCVVFLVLAGKELLSWGAGL
                     VAGLLYALWPAGASASVNLLHDHSPQPFFLFAGLWLVLCALRLERPALLFAGACLLGL
                     PALFRADGSVVGAALALGVAAVVWNARRAPGRALAWGALAFAGVLAPSLPWDAWTFSR
                     TGRLQHIAMGDAGAAVFMRFGHASGFAGARYNDSEVAFLGGSSEPFGPDWTDARRQRI
                     PGDFGRTLFWADPVRYARLVRSEMRSVATERAGRAAWWAPDSRNSEASRATEPSGRLE
                     AWLARLGRSRLLSSPVDTPWIYLLGVLGTVSGLIARRPTVVVPAVVGLFWMAALTVLL
                     PNARYLLMPSCFLFPLMGVLFASVARVFVQGVGSLRRAGAEGKTR"
     gene            3426..3761
                     /locus_tag="PLB01_02060"
     CDS             3426..3761
                     /locus_tag="PLB01_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76112.1"
                     /translation="MRLVSLPDGRAVTLRESTDILMRPRFAPDGASFTFVERTSSGWH
                     VRRRRVDDGSETTLFRSSRFIESAEAHPDGRHLLLTLGDDAEFHGYMSGLHVYEMDLA
                     SGRLERIGE"
     gene            3758..4360
                     /locus_tag="PLB01_02065"
     CDS             3758..4360
                     /locus_tag="PLB01_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76113.1"
                     /translation="MIVRLPAILFLAGAAACLGAPAFLEPPVDEGLRVEALGVSAWTV
                     AGDTPRSAGDAFRSPAIRVRGETLVLGLLIEGEVARGGISYRFEDEAGRLLRPDGQAG
                     PGRLTDCALAVRADGRPSVRLVLANPADGGSRWKLSRVEVRQFREFECPGCARRLEER
                     FSLGRSAWLWAWGVLRSPAPRGDASRIPECACAGVRPQAR"
     gene            complement(4348..5481)
                     /gene="rffA"
                     /locus_tag="PLB01_02070"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(4348..5481)
                     /gene="rffA"
                     /locus_tag="PLB01_02070"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HQQ76114.1"
                     /translation="MNLISFNRPCLAGNEFAYMRLAVDGMRISGDGDFTKRAHSMLEG
                     LVGARRALLTTSCTHALEMVAHLLDLAPGDEVIVPSYTFVSTANAFALRSAVPVFADV
                     RKDTLNLDEAAVEGLVSPRTRAIVAVHYAGIACEMETLLEIAARRGLTLVEDNAHGFL
                     GAYRGRPLGSLGALATLSFHETKNFTCGEGGALLLNDERFVARAEILREKGTDRGRFF
                     RGEVDKYTWVDLGSSYLPSDLLAAFLVAQLEARERIQARRRAIWEAYAEGLDAWALRT
                     GARLPFVPPGCDPAWHLFFVVMPDEDARQRMLAHLADRGVNAVFHYVPLHLSPMGRRL
                     GGRSGSCPVSEWASARLLRLPLHLGLTDEDVARVVDAVTAYRA"
     gene            complement(5478..6392)
                     /locus_tag="PLB01_02075"
     CDS             complement(5478..6392)
                     /locus_tag="PLB01_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76115.1"
                     /translation="MKWSRVGLVFRPERQRPWLHSHASNPVALALGGTRVRVFFGTRD
                     SDNRSQVAFFDMDLAEPSRAVELCAEPVVTAGPRGAFDDSGVSMGCLVEVAGALHLYY
                     LGWNLGVTVPFRNSVGLALAAGPDGPFRKASAAPVLDRSQEDPLSVSYPWVLPDAGGF
                     RMWYGSTVSWGPGPADMTHVIRHATSPDGRAWARTPVPVLVPDGSGESVVVRPCVVRD
                     VDRFRMWFCARGPGYRLGYAESEDGLAWTRRDEDAGLAPSGSGWDSSEIAYPHVFDCA
                     GARWMLHNGDGFGRTGIGLAMLASGSGA"
     gene            complement(6397..7056)
                     /locus_tag="PLB01_02080"
     CDS             complement(6397..7056)
                     /locus_tag="PLB01_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009939784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HQQ76116.1"
                     /translation="MARIVIFGTGDIARLAHFYFKRDSPHEVVGFTVDAAFRTSDTFE
                     GLPLVAFESVATAFPPSEYGMFIALAYGRMNHLRAERYTQAKAAGYTLVSYVSSRCTF
                     LSDHPVGDNCFILEDNTIQPFVKIGSNVTLWSGNHIGHDSTIEDHCFLASQIVVSGRV
                     VIKPFCFIGVNATLHNAITIAPETLIGAGAVITKDTEPKGVYVPRRAERMPKSSDEIE
                     M"
     gene            complement(7068..7685)
                     /locus_tag="PLB01_02085"
     CDS             complement(7068..7685)
                     /locus_tag="PLB01_02085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017351437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ76117.1"
                     /translation="MTDPLRAVADYYAEKLRANGPGAAGVDWNSAESQALRFRELSRL
                     LPAEGSFSVLDVGCGTGALVDFLAAEGWRFSYWGCDVSDAMLQVARRLHAGRPDVRFA
                     ARAEDAPPCDFAFASGIFNVKLDADDDAWSRHMEATVRLMARQGLRGIAFNALSVYSD
                     PGKRRADLHYADPLKWFDFCKREISTSVALLHDYPLWEFTLLVRR"
     gene            complement(7682..8617)
                     /locus_tag="PLB01_02090"
     CDS             complement(7682..8617)
                     /locus_tag="PLB01_02090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ76118.1"
                     /translation="MKLSIVATLYESAATVREFVARSRAAAAALTDDFEIILVNDGSP
                     DSSLDEALALFEHDPCLRIVDLSRNFGHHLAMMTGLAHARGDLVFLLDSDLEEDPALL
                     ADFHRELLASDADVVFGVQSRRKGGVLERVSGSLFYSIFNRVSSVKVPRNVVTARLMT
                     RRYVDALVTHRDREVFLLGLWTITGFRQVPVAIEKKDKGRTTYGVGRKAAVLVNSLTS
                     FSNRPLILIFWAGCLILGCATIAAVDLVVRRLFFGTLLQGWPSLIVSIWLLGGFTIFC
                     LGVIGIYVAKIFTEVKERPYTVVRRVYEHAPPGRP"
     gene            complement(8614..9309)
                     /locus_tag="PLB01_02095"
     CDS             complement(8614..9309)
                     /locus_tag="PLB01_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HQQ76119.1"
                     /translation="MGKTVAIVQSNYIPWKGYFDLIRLSDEFILLDDVQYTRRDWRNR
                     NLIKTRTGTQWLTIPVASRGRYHGTIREMETSDPAWAEHHLRTLRHAYARAPHFESVT
                     AFLEGVYHRATDRHLHAINRLFLEAICGYLGISTPLTSSTPADAAAQPTDRLVALCRR
                     AGASVYLSGPSARDYLDETAFSRAGIAVTYMDYAGYPEYPQIHPPFVHGVSIVDLLFH
                     TGPGAAAFLRRES"
     gene            complement(9377..10939)
                     /locus_tag="PLB01_02100"
     CDS             complement(9377..10939)
                     /locus_tag="PLB01_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQQ76120.1"
                     /translation="MSGPEGSVERMEFPSGRAIAITTTLPNGKTRPDGVYTYELRAQR
                     SVVPAAPAVLSGNETADGRPVETDAATKARPAVPPPPLSGSFWIQNGSFVAGSALEPP
                     QRRGAPSTPGNASGAGRAANAGRTALDFFVNDDQIVVGSGCFGFDCVNNEVFGLENLL
                     LKQNNNRIRFDDTSVSAGFPNNDWQIRANDDNSGGLNRFSIEDLTAARIPFTILAGAP
                     ANSIFVDASGRLGVRTGTPAQTIHAAGGDTPGLRLEQNGSSGFTPQTWDVAGNESNFF
                     VRDVTGGNTLPFRIHPGAPSSSIDIATNGNVGIGTGAAAQKLTVMNGPIFVAATAANL
                     DVFAGMGIDLGSGPAFNYGYAGSSFGRSAGFFNVRPDASAVAPNPSLRFMTANVQRMI
                     ITNTGNVGIGVANPSQPLQMASGAFVSAGGVWTNASSRSLKDDIRDLPADEALETLQG
                     LSPVTYVYKAAPSEPHVGFIAEDVPALLASPDRKGMSPMDVAAVLTKVVQQQQKTIEE
                     LKARLDELEKKK"
     gene            complement(11130..12902)
                     /locus_tag="PLB01_02105"
     CDS             complement(11130..12902)
                     /locus_tag="PLB01_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQQ76121.1"
                     /translation="MKTMSRLLAPLVSFVALAGVAPAAEAQKEAAVRTPLLADVVAGS
                     SRLDFVPRTGSGAFVVTVSGPEGSVERIDFASGRTIAITATLPDGKSRPDGIYNYELR
                     AKAPAPPAPGPDFSGSESVNGRAVEIENAVRAPEPPAQSGTFRILNGMIVAGSATESR
                     PARSTPAAGATGAGRTPSAGRTALDQVIADDLIVQGSACIGLDCVNNESFGFDTIRLK
                     ENNTRIKFDDTSASAGFAFHDWQLTANDNASGGAEKFSIEDITAATVPFTVTGSAPSN
                     ALFVDSTGRVGLRTATPVLDLHANTSNTPAMRLEQNSGGGFTAQTWDIAGNEANFFVR
                     DVTGGSRLPFRIRPGAPTSSIDIAASGNVGIGTASPTQKLTVRDGPIFVQAAADNLDV
                     FAGMGVDLSSGPAFNYGYAGSSFGRSAGFFNVRPDASAVAPNPSLRFMTANVQRMIIT
                     NTGNVGIGVANPSQPLQMASGAFVSAGGVWTNASSRSLKDDIRDLPADEALETLQGLS
                     PVTYVYKAAPAEPHVGFIAEDVPALLASPDRKGMSPMDVAAVLTKVVQQQQATIAEQN
                     RQLAEQNRLLADLSSRVRVLEDRP"
     gene            13090..14415
                     /locus_tag="PLB01_02110"
     CDS             13090..14415
                     /locus_tag="PLB01_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQQ76122.1"
                     /translation="MTAGLDSLLERIRSREAVVGVVGLGYVGLPLALLFEEAGFRVAG
                     FDVDPAKPVALRRGESYLRHLGSDRIAAAFSRGRITASDRFDGLAGCDAIVICVPTPL
                     GRHREPDLSYIRGTADEIARRLRPGQLVVLESTTYPGTTREELLPRFTAGGLVCGEDF
                     FLAFSPEREDPGNATFHTRNTPKIVGGIDGPSQSAAVALYGAAIEEVVPVASPEVAEA
                     AKILENVFRAVNIALVNELKMILDRMGIDVWDVIAAAKTKPFGFMPFYPGPGLGGHCI
                     PLDPFYLTWKAAEHGVWARFIELAGEINTAMPRYVVDRTVEALNAAGKSVLGARVLVL
                     GLSYKPDIDDDRESPSFEIIELLREKGADVSYCDPYIPRARRVRKHDLQMTSVPCTAE
                     EFARYDAVLLSTAHRQFRDPSLYAGVKLVVDTRNVVPAAAVERGKLTKA"
     gene            complement(14904..15131)
                     /locus_tag="PLB01_02115"
     CDS             complement(14904..15131)
                     /locus_tag="PLB01_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76123.1"
                     /translation="MGVATTVGVGVEGGVGVGVDAGVGVEVEAGVGEAVAVGVGVEVG
                     VDVGVGMEVTTVNATSAERLRTPCTFVQTRA"
     gene            15271..16812
                     /locus_tag="PLB01_02120"
     CDS             15271..16812
                     /locus_tag="PLB01_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76124.1"
                     /translation="MAYPAPGDDDVDFTKPFSWEVPSALSFGLHVGTSRGRSDVFDSG
                     ETPYSSVRVPRLAAGTYFGRIWANLAGIWKYQDFTFGSNGAPVATLQSPASGSVIPAG
                     RTTFLWTPVPNATAYRLVVGTTYTGADVADSGPIAGTAFDVSNVPQGAFVFARVWTQK
                     WGEWRSSDAVLSTGPSILPSLVAPVDGAANVDTGRPFEWKPSRYTTRYRLEIGTNPGA
                     NDLHDSGEIAVEKRFVGPLPVGATLYGRLSTLILGVWVPTDFVFTVGANGSPDSSVVE
                     AALWATDFVRGMADDSNNPYSETPLARLLDVEGWSNALCVDYASVLLSSLVEMNIRQP
                     ARPLNVCLKPNSYDGHTLVEFQEPVGGTWMLLDPTFDLTVTRGSDGGWATAGDVSDAT
                     RAKRFSDLTFRFLGSRGSSLARAYYIDYPLLFLIVCGQGACPSVYPSSLPYMEAVTLP
                     LPYVDVYAIRGNPDAPMSVLIDGVPAQLIGDGMDGMSRVFWAGSITSADGSPVEVFRP
                     LRFVF"
     gene            complement(16828..18399)
                     /locus_tag="PLB01_02125"
     CDS             complement(16828..18399)
                     /locus_tag="PLB01_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76125.1"
                     /translation="MVLPYHAWDAWAFGEWSRLIAEEPRRFFFPDLYALTYQRPFFYV
                     LQGWLWATFGFHEALGRMLALAFAILCAWTTKSMARSLDGGRGTWRWACLLPFAIPDF
                     LTWCAAGTTDVPAAALLAATGAILLGPVRPVPKALLVLVLSTSAALTKPTAIPSLVGL
                     ACAAAVSWPGVASPRRRVLAGVVPILSSLGIAGAYFFWLASRLGISVRSAIVGELDGY
                     YADLASRARWSALYVPSWLGPAAGPILAVALLALTISIAFRRPVHAARTAAAVALLAL
                     GWRFATAGWGGPGSREFLVSIATLVLVAAAARGTAANRPVSAHALFFLVWAAPPCFVW
                     IAYLGYDARLLSPGWPPLLLLAWIAFEPAAARIAGTRERAFALAAGAVLAAASLRHVD
                     GLDLASARCVARSVIAAPLDTGALRECFAPGFERERHAVASALSQAGRSSIISNDGRL
                     RFFFPGQVAQMYPATCGDLSGYGAFALLTDPGSRRYMAAKGIPADPSVWAACRGPRAS
                     LVFQDPAVSVFTLSP"
     gene            complement(18462..19325)
                     /locus_tag="PLB01_02130"
     CDS             complement(18462..19325)
                     /locus_tag="PLB01_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQQ76126.1"
                     /translation="MARAQATRTWVSGVGDDANPCSRTAPCKTFAGAISKTAAGGEIN
                     ALDPGGFGAVTITKAITIDGGGGQIASVLVSGTNGIVVNAGASDVVTIRNIQLEGLGT
                     GLSGISFLNGAALHVQRVAIRGFGGVGIDFRPSGNAKLFVSDSSVLHSGAGIQVHPSG
                     GASFATIVDTTLSGNASFGLRAEGNSSTVAKNVTADGNINGFIAIDFSGALPLLSIDA
                     CVASHNSNAGVAAGFGGAGSAKVLLSNTSIFENANGLSFDANGQIQSYGNNRVFGNGL
                     DGTPSGTIPHI"
     gene            19469..21487
                     /gene="asnB"
                     /locus_tag="PLB01_02135"
     CDS             19469..21487
                     /gene="asnB"
                     /locus_tag="PLB01_02135"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQQ76127.1"
                     /translation="MCGVVGWLSLSSRPVDAALLARMRDAMVHRGPDGEGLWASSDGR
                     VGLAFRRLAIVDLSATANQPMFNEDGSVAVVFNGEIYNHQKLRAELSARGHRFRTDHS
                     DTEVIVHGFEEWGPAVLDRLEGMFAIGIWDARSRLLFIARDRVGIKPLYFTWAGGGFL
                     FASEIKALLSHPDVPRDLEDLSVYHYLSFLTTPAPLTMFRGIYKLPAGHHVVVDEAGT
                     LRATRYWDALPGRSPEAAEIAAMARAEGLPRAVERVRALLDEAVEKRLMSDVPLGVFL
                     SGGVDSSALTALMAQHASGPVRTFTVGFSDHEHLNELDYARLVSRKYGTEHHEVLVDE
                     AAMRAYLPSLVYSQDEPIADWVCIPLFFVSKLLRDSGTTVVLVGEGADEQFCGYTSYM
                     AYLDMYRRYWRPFSMLPGPLRRVAASLADVAASADDRHDRYLDIIARAGRDREAFWSG
                     ATVFPESRKSRLVDRARLRTPAFPQELATSGLVPPGFGSPDTFGVVKSFFGRIDAEAP
                     GSDFLTRMIYSEFKLRLPELLLMRVDKIGMSVSIEPRVPFLDHKLVEFTMGLPMGLKV
                     GDGVSKSLLKRAVRGLVPDEIIDRPKMGFGAPMVQWLRGDFGRAVEAEFAATRFFDRF
                     PARRAAVLDMLRRHRDGRADFALYIWTFYNAVAWFDSWIDGGREARVA"
     gene            21484..23499
                     /locus_tag="PLB01_02140"
     CDS             21484..23499
                     /locus_tag="PLB01_02140"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HQQ76128.1"
                     /translation="MKPLELLRRAAGKSPGYLARRAALEARRAIGARRLRRRLEAETA
                     ELVARRAGAASLREVWEKSSARALGFTAPQRDALRSLYAGRFAPEGARLAEDFERVLA
                     HTFDLLGSGPVALGPVIDWHLDFKSGLRFPSGVASQGIDYAQLDRPSDVKVPWELSRG
                     QHLTRLGQAWVLARDERAPREFEAEVRAWIAANPPGTGVNWACTMDVALRAVSWVWAL
                     ALFEGAPFSNGFEETILLSLHQHGVWIPGHLELAEVNGNHYLSDALGMIALGVAFDAT
                     PEGRGWSDAGARIFEEEILLQTTEDGAGIEGSVPYHRLVLEIFLVGRRLLDAAGRPLS
                     REFDARLARMFEFVEAYVTPEGLSPVVGDADDGRALVLGREDVRDHRALLSTGAVLFG
                     RGSFRARATKLHEDSLWLLGPEAASAWDALPDDEPSGTCGFPDAGFFVLRSATQYVFV
                     DAGPVGFKGRGGHGHNDCLSFEWHAFGRPLLTDSGAYVYTASPEWRNRFRSTAFHNTI
                     RVDGEEINRFPSALALWSLKNDAVPFGVALERGAEEDELVSAHTGYRRLADPVIVTRT
                     FRFDRRDARLSLRDRIAGAGSHRVEFFFHAAPGGAGRLDGFDLAISFEGGPAVRLSPR
                     SRTPLAWEKREGWFSPSYGIRIPRETWIAVLSAPVPAEVDWELVAVA"
     gene            23534..25633
                     /locus_tag="PLB01_02145"
     CDS             23534..25633
                     /locus_tag="PLB01_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HQQ76129.1"
                     /translation="MLQVAVSGGEVKVLDVPAPVVRPGAVLVRTSASVISVGTESAST
                     GGGGRESLVLKAIRNPALIRRVLDRVASHGLKSTVETVRTRLSNDQPAGYSCAGTVVE
                     SGAPDLAPGDRVACAGAGWANHAALNVVPRNLVARVPDAVPLEDAAFATLAAIALQGV
                     RRAAPTLGERVVVLGLGLLGQITVQLLRASGCVALGVDVRRDRVDRALTLGLADGFAT
                     SERDFVAGILERTDGVGADAVIVTAAGGDAALLNRSFEACRRKGRVVLVGDVPIRIQR
                     DRIYKKEIDFLISTSYGPGRYDPEYEEKGLDYPLAYVRWTEGRNLEESLRLMAVGALR
                     VGPLVDATYPVERAPEAYASLAVEPRPLGVVLTYGAEAQAPARVHVSTRAAAPASGVV
                     GIGVIGFGSYFRSALLPLLKAQPGVALVSACSRNGLTVRAAAEKDGFRRGTTDVREVL
                     ADPEVRAVYIATRHDQHFAIARDAVEAGKAVFVEKPMTMTAPEGRELADLVAAKGALL
                     TVGFNRRFSPHVVRLREALAPIASPRTMLYRVNAGPLPKDHWLLDPAEGGGRLLGEGV
                     HFFDLLRFLAGSEIERVTSAPGANANDEGTVTLSFRDGSRGTLVYTGSGSAEAGKERL
                     EVFAGGSAFVLDDFHSLKSFGARKETFATKTVDKGQAAQLANFLAALRGEADLGVTAE
                     DGYWATWCAERARGATA"
     gene            25630..26853
                     /locus_tag="PLB01_02150"
     CDS             25630..26853
                     /locus_tag="PLB01_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQQ76130.1"
                     /translation="MSRRVPGPALYALSYAARLTTLGVGFLIGVVSARALGTDGRGAY
                     AELLVGVGLVVQLTNAGISTALGYAVARRPRRLSISISAACMSAVSAVVLSAVVVMLV
                     PGAFGRLGGLAIAWTGIQFLQIQQERLLYSVQRFVAANALLLGGRVLAFLIGFATWRV
                     RPMDVETFALGQAAAEGVTALVGFVLLRRMAGPWRWPRRPLVAAGARVALRAFPSLLL
                     PFLLLRSDILLLGVMRGARETGLYAVAVQLVDVLLLLPSSLASMLFPALFSIPDAAAY
                     TARVTLRGVAMLALLAGVLAAVAPFVIPRLFGPAFAPAVRPCLLLLPGAVLLGGQTLL
                     SQFFMRQGVPLFMSGFWAIGLAVNFGLNLFLIPGWGMSGAAVASSVAYTIVAALMAAR
                     FLRFWRGEAAKEAGA"
     gene            26853..28490
                     /locus_tag="PLB01_02155"
     CDS             26853..28490
                     /locus_tag="PLB01_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQQ76131.1"
                     /translation="MDAVVSVYPPAGSTGTQEELLALSMLRSLGASAALGTLRKRRYA
                     RAVFRLGEGDPPEIEAVAHVVLGVMRADSKGIERAGGAFAPLTIPGLLRAFAGLVVAG
                     VLGMAAAVANAWDVRSSRKPGGRRLGPGRTVAYLRASFGLPRVGGSVGHTSGVVNALA
                     QTGLEVRVFACAEPVGLKGRLSFTAVPLPRSATYPHELNAHRFGRRFFRACARSLSPR
                     AVRFLYQRYAINDLSGVRLARRLHRPLVLEYNGSEVWAQKNWGRALFLPGLARRIEVA
                     CLRESDLVVTVSEPLERELLDLGLPPHRVLFYPNCVDADVFDPARFDADARLRTRARF
                     GLDPGDFVITFVGTFGRWHGAEVLARAIRLLPEIVAGRRLRFLFVGDGVTAPETREVL
                     SAERVSGRAVFAGPRPQAETPEILAASDTFASPHVPNPDGTAFFGSPTKLFEYMAMAR
                     PIVASDLDQIGRILRNWQPGSVPAGASKAPLAILVEPGDPGSLAEGLQRAASLDRDEA
                     RRLGEAARAAILRSFRWEACVEAVVARLAAIDRERDA"
     gene            28487..30850
                     /locus_tag="PLB01_02160"
     CDS             28487..30850
                     /locus_tag="PLB01_02160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ76132.1"
                     /translation="MTPGGTERTRWTDPQRLLVTLLFTYLPFAMSGHLLVNHVLGGPW
                     YWYSAFLYPEGVLTVVVCVVTLCRAGERERLFDRLSGPGGLWWILGVAGAFVVWLAAS
                     AILQRAPTGFWIERLLAGWVLPMALALALLARADGVSDAWAGLRLGLAAFVPFALALY
                     LFSFGIPSSFRELVVLNRTWKMVSGVRGKVYFGELTLGGINDVAVFVAAALALAGGLW
                     LVDRRSRASRRVLWAWFVAGLVLELLCYSRGAIVSLALVAAFYAAASWRFVRLRALRV
                     PAALLTALVAVALALPGAWPYWSNQLRVGPGSTARVRLTLWRRILTVNAETATFGGQL
                     PAPPPPKPTPSVAPPPPAEPPAARAAGAGSATAAPVVEEARERIARFTSANGRQRVLG
                     FGLGNYGLLQGMTEDACTHSMFLDALAHGGLPGAVFFAAFWCLGLGLAARAVLLRPVP
                     SIGTPVPASISVAAALCVLTFAGGTVSFQFWNLGTMLNGAFVWLLVITSAATFREAQD
                     RKGQRWKAETVEQWGADPCGGVEASEFGTPEYFTAVTKERYEIYAPWLPAAAGFSRAA
                     GVRLLEVGFGLGTDLVSFSRGGASAFGVDLTPLHVFATRRRFLQQGLPVRVVRGDAER
                     LPFKAGAFDRVYSFGVLHHTPGIETALAEIHRVLTAGGGLTLGLYHRDSLYWAYQLLV
                     RGILKAGFLRHGYRRTMAGVEKSSRPDAETLVRAYSQKDVRLLLRAFRGVRLSVEHFS
                     FDQGGRIGLVLGRILRPFEDRLARKIGWYVMADARKQDGGVSAERAS"
     gene            30847..31947
                     /gene="wecB"
                     /locus_tag="PLB01_02165"
     CDS             30847..31947
                     /gene="wecB"
                     /locus_tag="PLB01_02165"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQQ76133.1"
                     /translation="MKILHVVGARPNFMKIAPVMEALSRRSGVVQRLVHTGQHYDEKM
                     AGIFFEQLGLPRPDRDLEVGSGTQAEQTAAVMVKFEKVLGEERPDVVVVVGDVNSTLA
                     ATLVAAKAGIPVAHVEAGLRSGDLSMPEEINRLVTDRLADILLTPSRDADDNLKKEGV
                     APGRIHFVGNVMIDTLLRHRARAPWDDVSGRLSLRDRGYSVLTLHRPANVDAADALAR
                     ILSRIEPVAAEYPVVFPVHPRTARRMTESGIEPPAGSVRLVEPLGYLEFLALMDHAAL
                     VLTDSGGIQEETTVLGVPCFTLRDNTERPVTITEGTNTLVGADAAGLAGAYAALRAGT
                     RRAARMPELWDGRAGERIADVLLGAVSRGSSL"
     gene            complement(32811..33266)
                     /locus_tag="PLB01_02170"
                     /pseudo
     CDS             complement(32811..33266)
                     /locus_tag="PLB01_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
     gene            33523..34743
                     /locus_tag="PLB01_02175"
     CDS             33523..34743
                     /locus_tag="PLB01_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017028501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQQ76134.1"
                     /translation="MNILFLSDNFPPERNASATRVHERACYWVREGHRVTVITCAPNF
                     PDGKVFPGYRNRGSVEDLDGIRVVRVRTFVSANRGFFLRTADFVSYMLAAVVAGLREE
                     EPDLVAATSPQFFAAVGGWILARLRRVPFVFELGDLWPASIEAVGASRSRVLLSLLES
                     VELFLYRQSAAVAALTHRFREDLIRRGIPGERIAVVRNGVDLPRYAPRPRDVSLSRAW
                     GLDGRFVVGYLGTHGMAHGLANVLAAADGLRGSDATFLFVGAGAERDALVQDAARRGL
                     DNVVFAPAQDKGEMPRVWSLCDVALVHLKDDPVFETVIPSKIFEAMGMGLPVLLVSPP
                     GEAREIVEAEGAGIWVPAGRPEALADAVNLLRTDEDLRRRLAAASLAAAPRHSRKRQA
                     DEMLEVFSRVLAEV"
     gene            complement(34748..35770)
                     /locus_tag="PLB01_02180"
     CDS             complement(34748..35770)
                     /locus_tag="PLB01_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQQ76135.1"
                     /translation="MTAAGVALGAGVAFFASALLSVLLARSRSSFWPRDVPNARSLHA
                     QPTPRTGGVAILLGVAAALAVAGATADTSFAWIGAATTLLALLSFADDRRGLPVSVRL
                     AAQALAAAALVWGAGLAAQRVWLPLAGDVALGPFAAPLSFLGILWLTNLYNFMDGMDG
                     LAGSMTAIGGAFLGVACWRGGDGGGAAVSFFLSAAAAGFLIQNRPPARIFLGDVGAIP
                     VGFLVAALALRASAAGRVDLGASCLVFAPFIVDATVTLVRRALAGERVWTPHRSHHYQ
                     RLVLSGLSHARVLRWEIALMGVSGAAALAYATGSGPVRVTILAALALLYAVAGAAVKA
                     VERARR"
     gene            complement(35767..>36807)
                     /locus_tag="PLB01_02185"
     CDS             complement(35767..>36807)
                     /locus_tag="PLB01_02185"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ76136.1"
                     /translation="LARRPERGPEWAGAEGGARRASGVHLVTGAGGFIGRALVESLAA
                     GGEPVRAMFRTPPQALPSGAESITADLSDPLALRRAVAGCDSVFHLAGKAHDLDAATR
                     SDAFRAANVDGTRSILEAALEAGTASFVFASSVKAMGEGGEECLDEEAPASPRSPYGR
                     SKLEAEGLVLEAGARSRMRATVLRLPLVYGPRMKGNLLSMLDAIESGFFPPLPGVANR
                     RSLASVGDVVQAFRLSAAAPAAAGRTYLVTDGVDYSSRGIYDAMREALGKRPVRWSVP
                     LTALRAAAAFGDAAAGATGRRVPFGRAALEKLLGSACYRNERIRRELGFRPVTTLQAA
                     LPEIVRIRRSGP"
BASE COUNT         5317 a        12356 c        13319 g         5815 t
ORIGIN      
        1 gagcgccggc ccgtccgcgc cgcgggtgtc gaggacgcgg cagggcgtca ggctgcacag
       61 agtcgcggcc ggcggcagcg tcacggcgta gtcgatgcgg cgcacctggc cgccgcccgc
      121 gtacgtcgtg tagtagagcg acgtccccgt cggcgacggc ccgaagagca ggtcgacgac
      181 ggtcgaggac ccgagcccgg tcgcgaaatc ggaagccgag ggcggccccc ccggcgtgag
      241 acgcgcgatc gcgccgcagt tgaaatcggc gaacaggtac gtcccgtcgt agctcgcggg
      301 ccacgcgccg gccggcaccc acgcgccgcc cgtgatcgac ccgcagcccg acgtcgtcgt
      361 ccccgggatc gtcccgtgcg ggaactcgaa gtacggatcg aggaggcccg cgggagtcgg
      421 gctgcacttg cccgacgtga gcgccgcgtg tgcgccctcg cggcagggcc agccgtagtc
      481 gcccccggcc acgatcggga agtcgatctc ctcccacgcg ccctggccga cgtcgttcac
      541 ggtgagcgcg ccgtcggagg gccggaacgc gagacggaac gcgttgcgga agccccacgc
      601 gaacgtctcc tggcagatcg tgcccggcgc ggaggggccc gtgttgcagc gcgcggtccc
      661 cgcgccgagg aacgggttcg tgggcggcac ggagccgtcc gtcgcaatgc ggacgatctt
      721 cccgttcagg atgttgcggt cgcgcgaagc gtcgttcgcc ccggcgcagc cgctgtcgcc
      781 cgcgtagtcg cacccgccgt cgcccgtgcc cgcgtagaga tacccgtccg gtccgacgcg
      841 cagctggccg ccgttgtggt tccccgcgaa gttcgtcacg ccgtcgagga ggacgacttc
      901 cgttgccggg ctgatgacgt tcgcggtggg gacgtcgagc gtgaagcgcg agatccgctc
      961 gacgggcgtc gtcgcgttgc tccccgggca tcccgccgcg ttcttgttga acgtgtagta
     1021 gaggtagacg tagtggttcg tcgcgaactg cgggtcgacg gccacaccga ggagaccacg
     1081 ctcgctgttc gagcacacgg ccgagccgag cgagagcgcg ggcgttggga gcagcgttcc
     1141 gcccgagacg atgcgcacgg tgccgccctg cgtcgtcacg agcaggcgtc cgtcgggtgt
     1201 gaacgcgagg gccgtcggcg ccccgacgtt ggccaccagg gcgtcgccga agccgggggg
     1261 gagggtgacc gcggaggcgg ggagggaaac gagggctgcg gcggcgagcg cagaggccag
     1321 cgagcggaga gcggattgat acgacatctc agggggaagt gttcggaggc cacgatcggg
     1381 cgacgatccg gattctggcg gcggcgagac gctttgttac tctttccggg cctcctgagg
     1441 agacacggta cggatcgtcc cgcatttcgc cccggcggcc gcctttcccg gctcctcctt
     1501 ggcacggcaa cgctgctgct ggtggcggta gcctgttcgt accggctgga cctcgcgcgc
     1561 acccacaccg tggccggcat cgaagggctc gctccgtggt ggacgtacgt tctgaagaac
     1621 gtcgattcgc tggacgaggg gagggggctc gtgagcgggt cgctctacgc tcccggccag
     1681 tcaatcgcgt cctggatcgt gctggcggtt ctggacggcc gggacgaccc ggcagtgctg
     1741 cggatcgtct tcggattcct ggacggcgtc tgcgtcgtct tcctcgtcct tgcggggaag
     1801 gagctgctct cctggggcgc cgggctcgtg gccgggcttc tctacgccct ctggccggcc
     1861 ggcgcgagcg cctccgtcaa cctcctgcac gaccactcgc cgcagccgtt cttcctcttc
     1921 gcgggcctgt ggctcgtcct gtgcgcgctg cggctcgaaa ggcccgccct tctcttcgcg
     1981 ggagcgtgcc ttcttgggtt acccgcgctc ttccgggcgg acggatcggt cgttggcgcc
     2041 gccctcgcgc tcggggtcgc ggcagtggtg tggaacgcac ggcgcgcgcc ggggcgcgcc
     2101 ctcgcctggg gcgcgctcgc gttcgccggc gtcctcgccc cgagcctgcc gtgggacgcg
     2161 tggacattca gccggacggg gcggctccag cacatcgcga tgggcgacgc gggcgcggcc
     2221 gtgttcatga ggttcggaca cgcgtcgggc tttgccgggg cgcggtataa cgattcggag
     2281 gtggcctttc tgggcggctc cagcgagccg tttggtccgg actggaccga cgcgcgccgc
     2341 cagcgcatcc ccggggactt cggccgaacg ctcttctggg cggacccggt ccgctacgcg
     2401 cgcctcgtcc ggagcgagat gcgctcggtc gcgacggaac gcgccgggcg cgccgcgtgg
     2461 tgggctccgg actcccggaa cagcgaggcg agccgcgcga cggagccgtc gggccggctg
     2521 gaggcctggc tcgcccgcct cgggcgctca cggcttctct cgtcgcccgt cgatacgccg
     2581 tggatctact tgctgggcgt tctcgggact gtgtcgggcc ttatcgcgag gcgaccgact
     2641 gtcgtcgttc cggcggtcgt cgggctcttc tggatggccg cgctgaccgt cctcctgccc
     2701 aacgcgcgct atctcctgat gccgtcctgt tttctctttc ccctcatggg agtcttattc
     2761 gcctcggtgg cccgcgtctt cgtccagggc gtcggctcat tgcgccgggc gggcgccgag
     2821 ggaaagacgc gatgacgcgg ttgcgggctg ccccggccct cttcgcggcg attgcgctcc
     2881 tggtggctgg aggcgctgtc gcacggcgag tggagcgagc gcgcggggcg ctgcgtggcg
     2941 gcacgtcgtt cgggtcgagc tacgtgccgc cgctgttcga agagatcgtc cacacgcggc
     3001 tgcgctactc cgacggaaca tacgcggtcg tcgtccggcc gcgtgccggc ggagagccta
     3061 cggttctatc cgggtcaagt cgaaccgccg cccgcgggct cgcgaaccgt cacgggttcg
     3121 tcgtatggag cgtcgacggg aaccggacga tgttccggtc cggcgcgcga ggggccgagc
     3181 cccctgcagt ggagcgatac gcgacctctc tgctcgatgg gatgggggcc gaaggccgtc
     3241 tcgcgacgct ccggtgccgc gacgagcttg aaactctcga cctcacaacg ggtcgggtgt
     3301 cgccgatacc cgcctctcgg cgcctcctgg acggtcttcg gccgcagcag cttcgattcg
     3361 cgcaatgggc gctcggactg ggcggccggg cggtcctcgt ccggccggta ggggatcgct
     3421 tcgcgatgag gctcgtgtcc ctgcccgatg gccgcgccgt cacgctgcgc gagtcgacgg
     3481 acattttgat gagaccgcga ttcgcgccgg acggcgcgag cttcacgttc gtcgaacgca
     3541 cgtcgtccgg atggcacgtg cgccgccggc gggtggacga cgggagcgag acgaccctct
     3601 tccgttcctc ccgtttcatc gagagcgccg aggcccatcc ggacggccgc cacctcctcc
     3661 tgacgctcgg agacgacgcg gagttccacg gatacatgtc cgggctccac gtctacgaaa
     3721 tggacctcgc atccgggcgg ctcgagagga tcggagaatg atcgtgcggc tgcccgcgat
     3781 cctgttcctg gcgggcgcgg cggcgtgtct cggcgcgccc gctttcctcg agccgccggt
     3841 cgatgagggg ctgagggttg aagcgcttgg agtttcggcg tggaccgtgg ccggcgatac
     3901 gccgaggtcg gccggggacg cgttccggag cccggcgatt cgcgtgcgcg gagagacgct
     3961 cgtcctgggc ctcctgatcg agggcgaagt agcgcgcggg ggaatctcgt accgcttcga
     4021 ggacgaggcc ggccgcctcc tccggcccga cgggcaggcg ggtccgggcc gcctcacgga
     4081 ctgcgcgctt gcggtccggg ctgacgggcg gccgtcggtc cggctcgtgc tcgcgaatcc
     4141 ggccgacggc ggttcacgct ggaagctctc ccgggtcgag gtgcggcagt tccgcgagtt
     4201 cgagtgcccc ggctgcgccc ggaggctcga ggagcggttc tctctcggcc ggagcgcgtg
     4261 gctctgggca tggggcgttc tacggagtcc ggctccccgc ggagacgcgt cccggatccc
     4321 cgagtgcgcc tgcgcgggcg tccggcctca ggcgcggtag gccgtcacgg catccacgac
     4381 gcgcgcgacg tcctcgtccg tgagcccgag gtgcagcggc aggcgcagga ggcgcgcgct
     4441 cgcccactcg ctcaccgggc aggacccgga gcggccccct aagcggcggc ccatcggcga
     4501 aaggtggagc gggacgtagt ggaagacggc gttgacgccg cggtccgcga ggtgggcgag
     4561 catccgctgt cgcgcgtctt cgtcgggcat gacgacgaag aaaaggtgcc acgccgggtc
     4621 gcagccggga ggcacgaacg gaagccgggc gcccgtgcgg agtgcccatg cgtcgaggcc
     4681 ttccgcgtac gcctcccaga tggcgcgccg tcgggcctgg atccgctcgc gcgcctcgag
     4741 ctgcgcgacg aggaaggccg cgagcaggtc ggacggaaga tagctcgagc cgagatcgac
     4801 ccaggtgtac ttgtccacct cgccgcggaa gaagcggcct cggtccgttc ctttctcgcg
     4861 gaggatctcg gcgcgcgcga cgaagcgttc gtcgttcaga aggagcgcgc cgccctctcc
     4921 gcacgtgaag ttcttcgtct cgtggaagct cagggtcgcg agagcgccga gcgagccgag
     4981 gggccgcccg cggtacgcgc cgaggaaccc gtgggcgttg tcctcgacga gagtgagccc
     5041 gcggcgagcg gcgatctcca ggagcgtttc catctcgcag gcgatcccgg cgtagtggac
     5101 ggccacgatc gcccgggtgc gcggcgacac gagcccctcg acggcggcct cgtcgagatt
     5161 cagcgtgtcc ttccgcacgt cggcgaagac ggggacggcg gagcgcagcg cgaacgcgtt
     5221 tgccgtcgag acgaacgtgt acgaggggac gatgacctcg tctccgggtg cgaggtcgag
     5281 aaggtgcgcc accatctcga gcgcgtgcgt gcacgacgtc gtcaggaggg cccggcgggc
     5341 gccgacgaga ccctcgagca tggagtgggc cctcttcgtg aagtcaccgt cgccggagat
     5401 ccgcattccg tcgacggcca ggcgcatgta ggcgaactcg tttcccgcga ggcacggccg
     5461 gttgaaggag atcaggttca cgcgccgctc ccgctcgcga gcatcgcgag gccgatgccg
     5521 gtccgcccga acccgtcgcc gttgtggagc atccagcgag cccccgcgca gtcgaacacg
     5581 tgggggtagg cgatctcgga ggagtcccat cctgaacccg aaggggcaag ccccgcgtcc
     5641 tcgtcgcggc gcgtccaggc gaggccgtct tccgactcgg catagccgag ccggtatccc
     5701 ggaccgcggg cgcagaacca cattcggaag cggtccacgt cgcggaccac gcaggggcgc
     5761 accacgacgc tctcgccgga cccgtccggg acgagaacgg gaaccggggt gcgcgcccac
     5821 gcgcgtccgt cgggcgacgt cgcgtgccgg atcacgtgcg tcatgtcggc tggtccgggt
     5881 ccccaactca cggtcgagcc gtaccacatc cggaagccgc ccgcgtcggg gaggacccac
     5941 ggataggaaa cggacaaggg atcctcctgt gagcggtcga ggacgggggc cgcggacgcc
     6001 ttgcggaagg ggccgtccgg cccggcggcg agcgcgaggc cgacgctatt ccggaacggg
     6061 accgtgacgc cgaggttcca gccgaggtaa tagagatgga gagcccccgc gacttccacg
     6121 aggcacccca tcgacacgcc gctgtcgtcg aacgcgccgc gcggaccggc agtcacgacc
     6181 ggttcggcgc agagttcgac agcacgcgac ggctccgcga ggtccatgtc gaagaacgcg
     6241 acctggctgc ggttgtcgga gtcccgggtt ccgaagaaca cgcggacacg cgtcccgccg
     6301 agagcgagtg cgaccgggtt cgaggcgtgc gagtggagcc acggccgctg gcgctcggga
     6361 cggaagacga ggccgacgcg gctccacttc atctggtcac atctcgattt cgtcgctgga
     6421 cttcggcatg cgttcggcgc ggcggggaac gtaaacgccc ttcggttccg tgtccttcgt
     6481 gatgacggca cccgccccga tcagcgtctc gggcgcgatc gtgatcgcgt tgtggagcgt
     6541 cgcgttcacc ccgatgaaac agaacggctt gatgacgacg cggcccgaga cgacgatctg
     6601 cgaggcgagg aagcagtgat cctcgatcgt cgagtcgtgg ccgatgtggt tgccgctcca
     6661 gagcgtcacg ttggacccga tcttcacgaa cggctgaatc gtgttgtctt cgaggatgaa
     6721 gcagttgtcc ccgacggggt ggtcgctcag gaacgtgcac cggctgctca cgtagctcac
     6781 gagcgtgtag cccgccgcct tcgcttgcgt gtagcgctcg gcgcgcagat ggttcatccg
     6841 gccatacgcg agggcgatga acatcccgta ctcggagggc gggaatgcgg ttgcgaccga
     6901 ctcgaaggcg acgaggggaa gtccctcgaa ggtgtccgac gtgcgaaacg cggcgtccac
     6961 cgtgaacccg acgacctcgt gggggctgtc gcgcttgaag tagaaatggg cgagccgggc
     7021 aatgtcgccg gttccgaaga tcacgatgcg ggccacggga acctccctca gcgcctgacg
     7081 aggagcgtga actcccagag cggatagtcg tgcaggagag cgacggacgt cgagatctcc
     7141 cgtttgcaga agtcgaacca cttcaaggga tccgcgtagt gaaggtccgc ccgccgcttc
     7201 ccgggatcgg agtagacgga aagcgcgttg aaggcgatcc cccgcaggcc ctgacgggcc
     7261 atcaaccgaa cggtggcctc catgtgccgc gaccacgcgt cgtcgtccgc gtcgagcttg
     7321 acgttgaaga tcccgctcgc gaaggcgaag tcgcacgggg gcgcgtcctc ggcccgtgcg
     7381 gcgaagcgca cgtcaggcct gccggcgtgg agccgccgcg ccacctgcag catcgcgtcc
     7441 gacacgtcgc agccccagta cgagaagcgc cagccctcgg ccgcgaggaa gtccacgagg
     7501 gcgccggtgc cgcagccgac gtcgaggacc gagaacgatc cttcggccgg gagcagtcgg
     7561 gagagctcgc ggaagcgaag cgcctgcgac tcggcggagt tccagtcgac gccggcggcg
     7621 ccggggccgt ttgcgcggag tttctccgcg tagtagtcgg cgacggcgcg gagcgggtcg
     7681 gtcacggccg tcccggaggg gcgtgctcgt acacgcgtcg gacgaccgtg tacgggcgct
     7741 ccttgacctc ggtgaagatc ttcgcgacgt agatgccgat cacgccgagg cagaagatcg
     7801 tgaagccgcc gaggagccag atcgagacga tgagggacgg ccagccctgc aggagcgtgc
     7861 cgaagaacag gcgccgcacg acgaggtcga cggccgcgat cgtggcacag ccgaggatga
     7921 ggcagccggc ccagaaaatc aggatgagag gccggttgct gaaggacgtc agcgagttga
     7981 cgaggacggc cgcctttcgg cccacgccgt acgtcgttcg ccccttgtct ttcttctcga
     8041 tcgcgacggg gacctgccgg aagcccgtga tcgtccagag gccgaggagg aagacctcgc
     8101 ggtcgcggtg ggtgacgagc gcgtccacgt agcgccgcgt catgaggcgc gccgtcacca
     8161 cgttgcgcgg caccttcacg ctcgagacgc ggttgaagat cgaataaaag aggctgccgc
     8221 tgacacgctc caggacgccc cccttgcgcc gcgactggac gccgaatacg acgtcggcgt
     8281 ccgacgcgag gagctcccgg tggaaatccg cgaggagagc cgggtcctct tcaaggtccg
     8341 agtcgaggag gaagacgaga tcgccgcgcg cgtgggcgag acccgtcatc atcgcgaggt
     8401 ggtgcccgaa gttgcgcgag aggtcgacga tccgaagaca cgggtcgtgt tcaaagagcg
     8461 cgagcgcctc gtcgagcgag gagtcggggg agccgtcgtt cacgaggatg atctcgaagt
     8521 cgtccgtgag ggccgcggcc gccgcgcggc tacgcgccac gaattccctg acggtcgcgg
     8581 cggactcgta gagcgtcgcg acgatcgaga gtttcacgat tccctccgaa gaaacgccgc
     8641 cgcgccgggg cccgtgtgaa agaggagatc cacgatgctg actccgtgca cgaagggcgg
     8701 gtggatctga ggatattcgg gataaccggc gtagtccatg tacgtgacgg cgatcccggc
     8761 ccgtgaaaaa gcggtctcgt caaggtagtc ccgcgccgag gggccggaga gatagacgct
     8821 cgccccggcg cgccggcaaa gcgccacgag ccgatcggtg ggctgggccg ccgcgtccgc
     8881 aggcgtcgag gacgtcagag gcgtcgagat ccccaggtac ccgcagatcg cctcgaggaa
     8941 caggcggttg atcgcgtgga ggtgccggtc ggtggccctg tggtagacgc cctcgaggaa
     9001 agcggtgacg ctctcgaagt ggggcgcgcg ggcgtacgcg tgacggagcg ttcgcaggtg
     9061 gtgctccgcc caggccgggt cgctcgtttc catctcgcgg atggtcccgt gatagcggcc
     9121 gcgcgacgcg acggggatcg tgagccactg cgttcccgtc cgcgtcttga tgaggttccg
     9181 gttccgccag tcccggcgcg tgtactgaac gtcgtccagg aggatgaatt cgtccgagag
     9241 ccggatgagg tcgaagtacc ccttccacgg gatgtagttc gactggacga tcgcgaccgt
     9301 cttccccacg ccggcactct agcgaaaaag ggcgccggaa gaccggcgcc cgggaaacga
     9361 ccgctgatcg tttgcgctac ttcttcttct cgagctcgtc gaggcgggct ttcagctctt
     9421 cgatggtctt ctgctgctgc tggacgacct tcgtgaggac ggcggcgacg tccatggggc
     9481 tcatgccctt gcggtcgggc gaggcgagaa gcgccgggac gtcctcggcg atgaagccga
     9541 cgtgtggctc ggagggcgcg gccttgtaga cgtacgtgac gggggagagg ccctgcaggg
     9601 tctcgagcgc ctcgtcggcc ggaaggtcgc ggatgtcgtc cttgagcgag cggctggagg
     9661 cgttcgtcca cacgccgccg gccgagacga aggcgccgct ggccatctgg aggggctggc
     9721 tggggttcgc gacgccgatg ccgacgttgc cggtgttcgt gatgatcatg cgctggacgt
     9781 ttgcggtcat gaagcgcagg gacgggttcg gggcgacggc ggaggcgtcg ggcctgacgt
     9841 tgaagaaccc cgcgctgcgg ccgaaggagg agccggcata gccgtagttg aaggcgggtc
     9901 cggacccgag gtcgatgccc atgcccgcga agacgtcgag gtttgcagcg gtggccgcga
     9961 cgaagatcgg accgttcatc acggtgagtt tctgcgccgc cgcgccagtc ccgatgccga
    10021 cgttgccgtt cgtcgcgatg tcgatgctgc tcgacggcgc gccggggtgg atgcggaacg
    10081 gcagcgtgtt cccccccgtt acatcgcgga cgaagaagtt cgattcgttg ccggcgacat
    10141 cccatgtttg aggcgtgaag ccgctcgagc cgttctgttc gaggcgcagg ccgggcgtgt
    10201 cgcccccggc ggcgtggatc gtctgcgcgg gtgttcctgt ccgcacgccg aggcggccgc
    10261 tggcgtcgac gaagatggag tttgccggtg cgccggccag gatcgtaaac ggaatcctgg
    10321 cggccgtcag gtcctcgatc gagaaccggt tcagcccgcc gctgttgtcg tcgttcgccc
    10381 gaatctgcca gtcgttgttg gggaagccgg ccgagacgct cgtatcgtcg aagcgtatcc
    10441 ggttgttgtt ctgcttcagg agcaggttct cgaggccgaa gacctcgttg ttcacgcagt
    10501 caaacccgaa acagccgctc ccgacgacga tctggtcgtc gttcacgaaa aagtcgaggg
    10561 ccgtgcggcc cgcgttcgcg gcgcggccgg ctccggaggc gttccccggc gtcgacggcg
    10621 cccctcgccg ctgcggaggc tcgagggcgc ttccggcgac gaaggagcca ttctggatcc
    10681 agaagctgcc ggacagtggg ggaggcggta ctgccggacg tgccttggtc gcggcgtcgg
    10741 tttcgaccgg gcggccgtct gccgtctcgt tgccgctcag cactgcaggc gccgctggga
    10801 ccacagaccg ttgggcgcgc agctcgtatg tataaacgcc gtcgggcctt gtcttcccgt
    10861 tcggaagggt cgtcgtgatc gcgatcgcgc ggccggatgg aaactccatc cgctcgaccg
    10921 agccctccgg accgctcacg gtcaggacga aagctccgcc aaagctgcgt ggaacgaaat
    10981 cgagcctcga ggccccggcc acgacgtcca caggtggtgg tgcggcggca gcactctcgg
    11041 cgcgcgcggt cggggcgcaa gcggcgagga gaacgagggc cagggtgcgg gttggcaagt
    11101 acaccttcat cggttactcc ttcacgtatt cagggccggt cttccagcac gcggactcgc
    11161 gaagagagat cggcgagcag gcgattttgt tcggcgagct ggcgattctg ctcggcgatg
    11221 gtcgcctgct gctgctggac gaccttcgtg aggacggcgg cgacgtccat ggggctcatg
    11281 cccttgcggt cgggcgaggc gagaagcgcc gggacgtcct cggcgatgaa tccgacgtgg
    11341 ggctcggcgg gggcagcctt gtagacgtac gtgacggggg agaggccctg gagggtctcg
    11401 agtgcctcgt cggccggaag gtcgcggatg tcgtccttga gcgagcggct ggacgcgttc
    11461 gtccagacgc cgccggcgga gacgaaggcg ccgctggcca tctgcagggg ctggctgggg
    11521 ttcgcaacgc cgatgccgac gttgccggtg ttcgtgatga tcatgcgctg gacgttggcg
    11581 gtcatgaagc gcagggacgg gttcggggcg acggcggagg cgtcgggcct gacgttgaaa
    11641 aaccccgcgc tgcggccgaa cgacgagccg gcgtagccgt agttgaacgc ggggcccgaa
    11701 gagaggtcga cgcccatgcc cgcgaagacg tcgaggttat cggcggccgc ctggacgaaa
    11761 atcgggccgt ccctgacggt gagtttctgc gtcggcgagg ccgtgccgat gccgacgttg
    11821 ccgcttgccg cgatgtcgat gctgctcgtc ggcgcgcccg ggcggatgcg gaacggcagg
    11881 cgcgacccgc ccgtgacgtc gcggacgaag aagtttgcct cgtttcccgc aatgtcccag
    11941 gtttgagccg tgaagccgcc gccggaattc tgttccagcc gcattgccgg agtgttgctg
    12001 gtgttcgcgt gcagatcgag cacgggtgtc gccgtccgga ggccgacccg gcccgtggag
    12061 tcgacgaaca gggcgttcga cggcgcgctt ccggtcacgg tgaacgggac cgtcgccgcc
    12121 gtgatgtcct cgatcgagaa cttctccgcg ccgccgcttg cgttgtcgtt ggccgtgagc
    12181 tgccaatcgt gaaaagcgaa ccccgcggag gcgctcgtgt cgtcgaactt gatgcgcgtg
    12241 ttgttttcct tgaggcggat cgtgtcgaag ccgaaggact cgttgttcac gcagtcgagc
    12301 ccgatgcacg cgctgccctg cacgatgagg tcgtctgcga tcacctggtc gagggctgtc
    12361 cgaccggcgg acggcgtccg gcccgcgccg gttgctcccg ccgcgggcgt ggaacgtgcg
    12421 ggcctcgact cggtcgcgct gcccgcgacg atcatcccgt tcaggatccg gaaggtgccc
    12481 gactgggccg ggggctccgg cgcccggacc gcgttctcga tctcgaccgc gcgcccgttc
    12541 acggactcgg acccgctgaa gtctggcccg ggcgccggcg gcgcgggggc cttcgcgcgc
    12601 agctcgtagt tgtagatccc gtctggccgg gacttcccgt cggggagcgt cgccgtgatc
    12661 gcgatcgtgc gtcccgatgc gaaatcgatc cgttcgacgg atccctccgg accgctgacg
    12721 gtcacgacga acgcgccact gcccgtgcgc ggaacgaagt cgagccggct cgagcccgca
    12781 acgacatcgg cgagaagggg cgtccggacg gccgcctcct tctgggcctc ggcggcggga
    12841 gcgacgcccg ccagggcgac gaacgacacg agaggcgcca gcaggcggct catggtcttc
    12901 atggaaggac ctccttggtt cagcgtgaga ctcagttcag agcgcggtcg aaacgacgca
    12961 gacaattgaa tcgaattagt gcgcggacta tatcgcgtgg cggccggggc gggcaacggc
    13021 ccccgcggcc acgccggggt cgcgcgcgcg gccggtttgc tatcgtcgcc gcgccgcagg
    13081 gagggtgacg tgacggcagg gctcgacagc cttctggagc ggatccggag ccgcgaagcc
    13141 gtcgtcggcg tcgtggggct cgggtacgtg ggactccccc tggcgctgct tttcgaggag
    13201 gccggattcc gcgtcgccgg gttcgacgtc gacccggcca agccggtcgc cctgaggcgc
    13261 ggcgagtcgt acctgaggca tctcggctcg gatcggatcg ccgccgcctt cagccgcggc
    13321 cggatcacgg ccagcgaccg gttcgacggg ctggccggct gcgacgcgat cgtgatctgt
    13381 gtcccgacgc cgctcggtcg ccatcgcgag cccgatcttt cgtacatacg cgggacggcg
    13441 gacgagatcg cgcgccggct gcgcccgggg cagctcgtgg ttctcgagtc gacgacgtac
    13501 cccgggacga cgcgcgagga gctcctcccc cggttcacgg ccggcggcct cgtctgcggc
    13561 gaggacttct tcctcgcgtt ctcgcccgag agggaggatc ccggcaacgc gacgttccac
    13621 acgcgcaaca cgccgaagat cgtgggcgga atcgacggcc cttcgcagag cgccgcggtg
    13681 gcgctctacg gggctgcgat cgaagaggtc gttccggtgg cgtctcccga ggtggccgag
    13741 gcggcgaaga tcctcgaaaa cgtcttccgc gccgtgaaca tcgcgctcgt caacgagctc
    13801 aagatgatcc tcgaccggat gggcatcgac gtgtgggacg tgatcgcggc ggcgaagacg
    13861 aagccgttcg ggttcatgcc gttctacccg ggcccgggcc tcgggggcca ctgcatcccg
    13921 ctggacccct tctacctcac gtggaaggcg gccgagcacg gcgtctgggc gcgtttcatc
    13981 gagctcgcgg gcgagatcaa cacggcgatg ccgcgctacg tggtggaccg gaccgtcgag
    14041 gcgctgaacg ccgcggggaa gagcgtgctc ggcgcccggg tcctcgtgct cgggctctcg
    14101 tacaagccgg acatcgacga cgaccgcgag tcgccctcgt tcgagatcat cgagctgctc
    14161 cgcgagaagg gggcggacgt ctcgtactgc gacccctaca ttccgagggc gcggcgggtc
    14221 cggaaacacg acctgcagat gacctcggta ccgtgtacgg ccgaggagtt cgctcgatac
    14281 gacgccgtcc tcctctcgac ggcgcaccgg cagttccgag acccctccct gtacgcggga
    14341 gtgaagctgg tcgtcgacac ccggaacgtt gtcccggcag cggcggttga gcgcgggaag
    14401 ctgacgaagg cctgacggtt cttggtgggt cccacccgac aataaatgag ggcaggcccg
    14461 ggccgccgcc ggcccgtccg ggctttttcg gtgagatgcc tcacggtccc cgtcacttcc
    14521 tgtaaaccgg gcgacccgga cggcgtccat tcggttggta ctgaaagtgc atcgacacga
    14581 ggggttctcg tggaagaaag gtcggacttg agaagtcgtg tcttgatcca tgcccgccgc
    14641 gcggctctgc tgggcgcgct gcttgcggct ttcgcgtcgg gtacggcgct cgcggacccg
    14701 cttgccgccg agctgtcata cccggctccc ggcgcgaaga tctcggacct gagccagccg
    14761 ttccggtgga gcacggtctc ttccgcccag tcctacgttc tcgtcatcgg cacgggctcg
    14821 ggccagtccg acgtctggaa ttccggcgag acgacgcgga ccgaaggcct cgttcccgcg
    14881 aatctgccga aagggaagct cctctacgca cgcgtctgga cgaaagtgca gggggtcctc
    14941 aggcgctcgg cggacgtcgc gttcacggtc gtgacttcca ttcccactcc cacgtccaca
    15001 ccaacctcga cgccgacgcc gacggcaacg gcctcgccga cgcctgcctc cacctcgact
    15061 ccgacgcccg cgtccacacc aactcccacg ccgccttcga cccccacgcc gactgtggtg
    15121 gcgaccccca ctgccccgac tccgactccc actccgactc ccactcccac cccgacttcc
    15181 acttcaactc caactccgac gagtccgaat caacgggccg tcgtgaacct gcctcttcag
    15241 aggccttcgt cgagggtggt cggagcgtgg atggcttatc cagcccccgg agacgacgac
    15301 gtcgacttca cgaagccctt ttcctgggaa gttccgagcg cgctctcgtt cgggctccac
    15361 gtggggacgt ctcgcggccg cagcgacgtc ttcgactccg gtgagacgcc ctacagcagc
    15421 gtccgggttc cccggctcgc cgcgggcacg tacttcggcc gcatctgggc gaacctggcg
    15481 ggcatctgga aataccagga tttcacgttc gggtcgaacg gcgctccggt cgcgacgctc
    15541 cagtcgccgg ccagcgggtc ggtgatcccg gcggggcgga cgacgttcct ctggaccccc
    15601 gttccgaacg ccaccgcgta ccgtctcgtc gtcgggacga cgtacacggg cgcggacgtg
    15661 gccgactcgg gtcccatcgc cggaacggct ttcgacgttt cgaacgttcc tcagggggcg
    15721 ttcgtcttcg cgagggtttg gacgcagaag tggggcgagt ggcgctcgtc ggatgcggtc
    15781 ctttccaccg ggccctctat cctcccgtct ctcgtcgccc ccgtcgacgg cgccgcgaac
    15841 gtggatacgg gccgcccgtt cgaatggaag cccagccgct acacgacgcg gtatcgcctc
    15901 gagatcggga cgaaccccgg cgcgaacgac ctccacgaca gcggagagat cgcggtcgag
    15961 aagcggttcg tcgggccgct tcccgtgggg gcgacgctct acgggaggct gagcacgctc
    16021 atcctgggcg tctgggtgcc gaccgacttc gtcttcacgg tcggcgcgaa cgggtcgccg
    16081 gactcctcgg tcgtcgaggc ggcgctctgg gccacggact tcgtgcgcgg catggcggac
    16141 gattccaaca acccgtactc cgagaccccg ctcgcgcggc ttctcgatgt ggagggctgg
    16201 tcgaacgcgc tttgcgtgga ctacgcctcc gtcctcctct cgagtctcgt cgagatgaac
    16261 atccggcagc ctgcgcgccc gctcaacgtg tgtctgaagc cgaactcgta cgacggccac
    16321 acgctcgtcg aattccagga gcccgtgggc ggcacgtgga tgctgctcga tcccacgttc
    16381 gacctcacgg tgacgcgtgg ttccgacggc ggttgggcga cggcaggcga cgtgtcggat
    16441 gccacgaggg ccaagcgatt ctccgacctg acgttccggt tcctcgggtc gcgggggagc
    16501 tccctggccc gcgcgtacta catcgactac ccgctcctct ttctcatcgt ttgcgggcag
    16561 ggcgcctgtc cgagcgtgta cccctcgtcc cttccttaca tggaagccgt cacgctgccc
    16621 ctgccctacg tggatgttta cgcgatccgg ggcaatccgg acgccccgat gtcggttctc
    16681 atcgacggtg tgccggccca gctcatcggc gacggaatgg acgggatgag ccgggtgttt
    16741 tgggcaggct cgatcacgtc ggcggacggt tctcccgtcg aggtctttcg tcccctgcgc
    16801 ttcgtgttct gaccggagcc tggcgcttca cggggagagc gtgaagacgg acacggccgg
    16861 atcctggaag acgagactcg cccttggtcc ccggcacgcg gcccacacgg agggatcggc
    16921 gggaattccc ttagccgcca tgtaacggcg tgagcccggg tctgtgagaa gcgcgaaggc
    16981 gccgtagccc gagaggtcgc cgcacgtcgc cggatacatc tgtgcgacct gtccgggaaa
    17041 gaagaagcgc agacgcccgt cgttcgagat gatggacgat cgtccggcct gcgagagggc
    17101 gctggcgacc gcgtgccgtt cgcgctcgaa gcccggagcg aaacactccc gaagagcgcc
    17161 ggtgtcgagc ggggccgcga ttaccgaacg cgcgacgcac cgggcggaag cgaggtccag
    17221 gccgtcgacg tgccggaggc ttgccgcggc caggaccgcg cctgcggcga gggcaaaggc
    17281 cctctccctc gtgccggcga tccttgcggc cgccggctcg aacgcgatcc aggcgaggag
    17341 caggaggggg ggccatccgg gcgaaagcag gcgtgcgtca tacccgagat aggcgatcca
    17401 cacgaagcag ggcggagccg cccacaccag gaagaacagg gcgtgtgcgc tcacggggcg
    17461 gttcgcagcc gtgccgcggg cggcggcggc aacgagcacg agggtggcga tcgagacgag
    17521 gaactcacga ctcccggggc ctccccatcc ggcggtcgca aaccgccaac cgagggcaag
    17581 cagggcgacc gcggccgcgg ttcgggcggc gtgaaccggc ctgcgaaagg cgatggaaat
    17641 cgtgagtgcc aggagggcga ccgcaaggat cgggccggcg gcgggtccca gccaggaagg
    17701 cacgtagagg gcggaccagc gggcgcgcga cgcgaggtcg gcgtagtagc cgtcgagctc
    17761 tcccacgatt gcggagcgga cggagatccc gagccgcgac gcgagccaga agaaatacgc
    17821 cccggcaatg ccaaggctcg agagaatcgg cacgactccc gcgaggacac gtcgccgggg
    17881 ggacgcgact ccgggccatg agaccgcggc ggcgcaggcg agaccgacga gcgacgggat
    17941 cgctgttggc tttgtaaggg ccgcgctggt cgagagaacg agaacgagga gcgccttcgg
    18001 gacgggacgg acaggcccga ggaggattgc gcccgtcgcc gcgagaagtg ccgcggcggg
    18061 cacgtccgtc gtgcctgcgg cgcaccacgt caggaagtcg gggatcgcga acgggagcag
    18121 gcacgcccat cgccaggtgc ctctcccgcc gtcgagcgaa cgcgccatgc tcttggtcgt
    18181 ccatgcgcac aggatggcga acgcgagcgc gagcatgcgg ccgagcgcct cgtgaaatcc
    18241 gaacgtcgcc cagagccagc cctggagcac gtagaagaag ggtcgctggt aggtgagcgc
    18301 gtagaggtcc gggaagaaga acctgcgcgg ctcttcggca atcagtcgcg accactctcc
    18361 gaatgcccag gcgtcccagg cgtgataagg gaggaccacg aggacgcccg cacagaagcc
    18421 ggcgcccgcg aagagaacag gcggtgcgaa gcggcggaag gtcagatgtg cgggatcgtc
    18481 cccgagggcg tcccgtcaag gccgtttccg aagacccggt tgttgccgta ggactggatc
    18541 tgcccgtttg cgtcgaacga caggccgttc gcgttctcga aaatcgaggt gttcgacagg
    18601 aggaccttcg cggagccggc ccctccgaat cctgcagcaa cgcccgcatt cgaattgtgt
    18661 gaggcgacgc aggcatcgat cgagaggagc ggcaacgcgc cggagaagtc gatggcgatg
    18721 aatccgttga tgttgccgtc ggcggtcacg ttcttcgcca ccgttgacga gttgccttcc
    18781 gcgcggaggc caaacgacgc gttgccgctg agcgtcgtgt cgacgatcgt cgcaaaagaa
    18841 gcgcccccgg aaggatgcac ctggatgccg gcccccgaat gcaggaccga ggagtccgag
    18901 acgaagagct tggcgttgcc ggacggccgg aagtcgatcc cgacgccgcc gaagccgcgg
    18961 atcgcgacgc gctgcacgtg gagtgccgca ccgttgagga acgagatccc cgagagcccg
    19021 gtgccgagtc cttcgagctg gatgttccgg atcgtgacga cgtccgacgc acccgcgttg
    19081 acgacgattc cgttcgtccc ggagacgagc acgctcgcga tctgcccgcc tcccccgtcg
    19141 atcgtgatgg ctttcgtgat cgtcacggcg ccgaatccgc ccggatcgag ggcattgatc
    19201 tcgccgcccg ctgccgtctt gctgatggcc cccgcaaacg tcttgcaggg ggccgtccgg
    19261 ctgcacgggt tggcgtcgtc gccgacgccg gagacccacg tgcgggtcgc ctgggcccgt
    19321 gccatgcccg ggatgagact gagaccgatt agcgccacaa agacgccgac acgccgaatc
    19381 atcatgcgcc tccattcatg taacggattc tgtccccgtt cagcatacct cggaaggtca
    19441 cctcctcctc acgttagact cgatcgcgat gtgcggcgtc gtgggatggc tctccctttc
    19501 gagccggcct gtggacgccg cgctcctcgc gcgcatgcgc gacgcgatgg tgcatcgcgg
    19561 gccggacggg gaagggctct gggcgtcgtc ggacggccgt gtcgggctcg cgttccgccg
    19621 gctcgcgatc gtcgacctct cggccaccgc caaccagccc atgttcaacg aggacgggtc
    19681 ggtggcggtc gtcttcaacg gcgagatcta caaccaccag aagctccggg cagaactgtc
    19741 cgcccgcggg caccgtttcc gcacggacca ttccgacacc gaagtcatcg tccacggttt
    19801 cgaggagtgg ggccctgccg tcctcgaccg gctcgagggt atgttcgcga tcgggatctg
    19861 ggacgcccgt tcgcgcctcc tcttcatcgc ccgggaccgc gtcggaatca agcccctcta
    19921 cttcacgtgg gccgggggcg ggttcctgtt cgcctccgag atcaaggcgc tcctctccca
    19981 cccggacgtt ccgagggacc tcgaggacct ctccgtctac cactacctga gcttcctcac
    20041 gacgccggcg cctctcacga tgttccgcgg gatctacaag ctcccggccg gacaccacgt
    20101 ggtcgtcgac gaggccggga cgctgcgcgc aacgcgctac tgggacgcat tgccggggcg
    20161 cagccccgag gcggccgaga tcgcggcgat ggcgagagcc gaggggctgc cgcgcgcggt
    20221 ggagcgcgtg cgggcgcttc tcgacgaggc cgtcgagaag cgcctgatgt cggacgtccc
    20281 gctgggcgtc ttcctgtccg ggggcgtcga ctcctcggcc ctgacggccc tcatggcgca
    20341 gcacgcgagc ggcccggtcc gcaccttcac ggtgggcttc tccgaccacg agcacctgaa
    20401 cgagctcgac tacgcgcgcc tcgtgtcgag gaagtacggc accgagcacc acgaggtcct
    20461 cgtcgacgag gcggcgatgc gcgcatacct gccgtcgctc gtctactcac aggacgagcc
    20521 gatcgcggac tgggtctgca tcccgctgtt tttcgtgtcg aagctcctcc gggacagtgg
    20581 gacgacggtc gtcctcgtcg gcgagggcgc cgacgagcag ttctgcggct acacgagcta
    20641 catggcgtac ctcgacatgt accgccgcta ctggcggccg ttctcgatgc tgcccggccc
    20701 gttacgccgc gtcgccgcgt cgctcgccga cgtcgcggcg tcggccgacg accggcacga
    20761 ccgctacctc gacatcatcg cccgcgcggg tcgcgaccgc gaggcgttct ggagcggggc
    20821 gaccgtgttc ccggagtcgc gaaagtcgcg tctcgtcgac cgcgctcgcc tccgcacccc
    20881 ggcattcccg caggagctcg cgacctccgg tctcgtgccg cccggtttcg gctcgccgga
    20941 caccttcgga gtcgtgaagt cgttcttcgg ccggatcgac gccgaggccc ccggctcgga
    21001 cttcctgacc cgcatgatct actcggagtt caagctgcgg ctccccgagc tgcttctcat
    21061 gcgggtcgac aagatcggga tgtccgtctc gatcgaaccc cgcgttccgt tcctcgacca
    21121 caagctcgtg gaattcacga tgggcctccc gatggggctg aaggtcggag acggcgtttc
    21181 caagtctctg ctgaaacgcg cggtgcgcgg gctcgtaccg gacgaaatca tcgaccgccc
    21241 gaagatggga ttcggggcgc cgatggtgca gtggctgcgg ggagacttcg ggcgggcggt
    21301 cgaggccgag ttcgccgcaa cgcgtttctt cgaccggttc ccggcccgcc gcgccgccgt
    21361 gctcgacatg ctgcgccgcc atcgcgacgg acgcgcggac ttcgcgctct acatctggac
    21421 cttctacaac gccgtcgcgt ggttcgactc ctggatcgac ggcgggcgcg aagcgcgcgt
    21481 cgcgtgaaac cgctcgagct cctgcgccgc gccgccggca agtcccccgg gtacctggcc
    21541 cggcgcgccg cactcgaggc ccgcagggcg atcggcgcga gacgcctgcg ccggaggctc
    21601 gaggcggaga cggccgagct cgtcgcgagg cgcgcgggcg ctgcgtcgct gcgcgaggtc
    21661 tgggagaaga gcagcgcgcg cgccctcggc ttcacggcgc cgcagcggga cgcgctccgc
    21721 agcctctatg ccggacggtt cgccccggaa ggcgcgcgtc tcgcagagga cttcgagcgc
    21781 gtcctcgccc acacgttcga cctcctgggg tcgggacccg tggcgctcgg gccggtgatc
    21841 gactggcacc tcgacttcaa gtcggggctg cgattcccct ccggcgtcgc gtcgcagggg
    21901 atcgattacg cgcagctcga ccgtccgagc gacgtcaagg tgccgtggga gctctcccgc
    21961 gggcagcacc tgacgcgtct cgggcaggcc tgggttctcg cgcgcgacga gcgggctccg
    22021 cgcgagttcg aggccgaggt gcgcgcctgg atcgccgcga accctcccgg caccggggtc
    22081 aactgggcct gcacgatgga cgttgccctg cgcgcggtga gctgggtctg ggcgctggcg
    22141 ctgttcgagg gcgccccgtt ctcgaacgga ttcgaggaga cgatccttct ctcactgcac
    22201 cagcacggcg tctggattcc cgggcacctg gagctcgcag aggtaaacgg aaaccactat
    22261 ctctcggacg ccctcgggat gatcgcgctc ggcgtcgcct tcgacgcgac gcctgaaggc
    22321 cgcggctgga gcgacgcggg agcacggatc ttcgaagagg agatcctcct gcagacgacg
    22381 gaggacggcg ccgggatcga aggttccgtt ccctaccacc gcctcgtgct cgagatcttc
    22441 ctcgtgggcc ggcggctcct ggacgccgcg ggtcgcccgc tctcgaggga gttcgacgca
    22501 cgtctcgcgc ggatgttcga gttcgtggaa gcgtacgtga cgccggaagg actctcgccc
    22561 gttgtgggcg acgccgacga cggacgtgcg ctcgtgctcg gtagggagga cgtccgggat
    22621 catcgcgcgc tcctctcgac cggcgcggtc ctgttcgggc gcgggtcgtt ccgcgcccgc
    22681 gcaacgaagc tccatgagga ttcgttgtgg cttctcggcc cggaagccgc ttcggcatgg
    22741 gacgcgcttc cggatgatga gccgtccggc acgtgcggct ttccggacgc gggcttcttc
    22801 gtcctccgct cggcgaccca gtacgtcttc gtcgacgccg ggccggtcgg tttcaagggg
    22861 cgcggcggac atgggcacaa tgactgcctc tccttcgagt ggcacgcgtt tggccggcct
    22921 ttgctgaccg attcgggcgc ctacgtgtac acggcttcgc cggagtggag gaaccggttc
    22981 cgctcgacgg ccttccacaa cacgatccgc gtggacggcg aggagatcaa ccggttcccg
    23041 tccgcgcttg cgctctggtc gctgaagaac gacgcggttc ccttcggcgt cgcgctcgaa
    23101 cgcggggccg aggaggacga actcgtctcc gcacacactg gctaccggag gctcgcggat
    23161 cccgtcatcg tcacgcggac gttccggttc gaccggcggg acgcccgcct ctcgctccgg
    23221 gaccggatcg caggcgccgg aagtcaccgc gtcgagttct tcttccacgc cgcgccgggt
    23281 ggggcgggcc gcctggacgg cttcgacctc gcaatctcct ttgaaggcgg cccggccgtc
    23341 cgcctgtcgc ctcgctcgcg taccccgctc gcgtgggaaa agcgggaggg ctggttctct
    23401 ccgtcctacg gaatccgtat ccctcgggag acgtggatcg ccgtcctgtc ggcgcccgtt
    23461 cccgcggagg tggactggga actcgtggcg gtggcttgaa acgtccggcg gcgtcgagac
    23521 gctaagatcg ggcgtgcttc aagtggcagt ttcgggcggc gaggtgaaag tgctggacgt
    23581 gccggccccc gtcgttcggc ctggcgccgt cctcgtcagg acgtcggcgt ccgtcatcag
    23641 cgtgggaacg gagtcggcct cgaccggcgg cggcgggcgc gagagcctcg tcctgaaggc
    23701 gatccgcaat cccgcgctga tccggagggt gctcgaccgg gtcgcttcgc acggtctcaa
    23761 gagcacggtc gagaccgtcc ggacgcgcct ttcgaacgac cagccggccg gatactcctg
    23821 cgcggggacg gtcgtcgaga gcggcgcccc ggacctcgcg ccgggcgacc gggtggcgtg
    23881 cgcgggcgcg ggctgggcca accacgcggc gctcaacgtg gtgccccgca acctcgtcgc
    23941 gcgggtgccc gacgccgtcc cgctggagga cgcggcgttc gcgacgctcg cggcgatcgc
    24001 gctgcagggc gtgaggcgcg cggcgccgac gctcggcgag cgcgtcgtcg tgctcggtct
    24061 cggtctcctc gggcagatca cggtccagct cctccgtgcg agcgggtgcg tcgcgctcgg
    24121 ggtggacgtc cggcgcgacc gcgtggaccg cgccctcacc ctcgggctcg cggacggatt
    24181 cgcgacgtcg gagcgcgact tcgtcgccgg aatcctcgag cgcacggacg gggtcggcgc
    24241 ggacgccgtc atcgtgaccg ccgccggagg agacgccgcg ctcctgaaca ggtcgttcga
    24301 ggcctgccgc cggaaggggc gtgtcgtcct cgtcggcgac gtcccgatcc gaatccagcg
    24361 ggaccggatc tacaagaaag agatcgactt cctcatctcg acgtcgtacg gccccggccg
    24421 ctacgacccc gagtacgagg agaagggcct cgactacccg ctcgcctacg tgcgctggac
    24481 cgaaggccgc aacctcgagg agtcgctgag actcatggcc gtgggcgccc tgcgcgtcgg
    24541 cccgctcgtc gacgcgacgt atcccgtcga acgggccccc gaggcctacg cgtcgctcgc
    24601 cgtcgagccg cggccgctcg gggtcgtcct gacctatggt gccgaggcgc aggcgccggc
    24661 tcgcgttcac gtctcgacgc gcgccgcggc gccggcgagc ggcgtcgttg gaatcggcgt
    24721 gatcgggttc ggaagttatt tccggtcggc gctcctgccc ctcctgaagg cgcagccggg
    24781 cgtcgcgctc gtgtcggcgt gctcgcgaaa cgggctcacc gtgcgcgccg ccgccgagaa
    24841 ggacgggttc cgccgcggga cgacggacgt ccgcgaagtc ctcgcggacc ccgaggtgcg
    24901 cgccgtctac atcgcgacgc ggcacgatca gcacttcgcg atcgcgcgcg acgccgtcga
    24961 ggcgggcaag gcggtgttcg tcgagaagcc catgacgatg acggcgcccg agggacgtga
    25021 gctcgcggac ctcgtggcgg cgaagggcgc gctcctgacg gtcgggttca accgccggtt
    25081 ctcgcctcac gtcgtgcggc ttcgggaagc cctcgcgccg atcgcgtctc cgcggacgat
    25141 gctctaccgc gtgaacgccg gacctctccc gaaggaccac tggctcctcg accctgcgga
    25201 gggcggcggg cggctcctgg gggaaggggt gcactttttc gatctcctca ggttcctcgc
    25261 cgggagcgag atcgagcgcg tcacgtccgc gcccggagcg aacgcgaacg acgaggggac
    25321 ggtcacgctc tcattccgcg acggctcgcg aggcacgctt gtctacacgg ggagcggcag
    25381 cgcggaggcc gggaaggaac gcctcgaggt ctttgcgggc ggctcggcat tcgtcctgga
    25441 cgacttccac tcgctgaagt cgttcggagc ccggaaggaa acgttcgcca cgaagaccgt
    25501 cgacaaggga caagcggccc agctcgcgaa cttcctcgcg gcccttcgcg gcgaggccga
    25561 tctcggcgtc accgcggagg acggctactg ggcgacgtgg tgcgcggaac gggcccgggg
    25621 cgcgacggcg tgagccggcg ggttcccggt cccgcgctct acgcgctctc gtacgcggcc
    25681 aggctcacga cgctcggggt cggtttcctc atcggcgtcg tgtcggcccg ggcgctgggg
    25741 acggacgggc gcggcgccta cgcggagctg ctcgtgggcg tcgggctcgt cgtccagctg
    25801 acgaacgccg ggatctcgac ggcgctcggg tatgccgtcg cgcggcgccc tcggcgattg
    25861 tcgatttcca tctcggcagc gtgcatgtcc gccgtctcgg cggtcgtgct gagcgccgtc
    25921 gttgtgatgc tcgttccggg cgcgttcggg cgcctcggcg gactcgcgat tgcctggacg
    25981 ggaatccagt tcctccagat ccagcaggaa aggcttcttt acagcgtcca acgattcgtc
    26041 gcggcgaacg cgctccttct cggcggccgc gtcctggcgt tcctgatcgg gttcgcgacc
    26101 tggcgcgtcc ggccgatgga cgtcgagacc tttgcgctcg gccaggccgc cgcggagggt
    26161 gtcacggcgc tggtgggttt cgttctcttg aggcgcatgg cgggaccctg gcgctggccg
    26221 cgccggccgc tcgtcgcggc gggcgcccgc gtggcgctcc gcgccttccc gtcccttctt
    26281 ctcccgtttc tcctccttcg ttccgacatt cttctcctcg gcgtgatgcg gggggcgcgg
    26341 gagacgggtc tctatgcggt cgcggtccag ctcgtggacg tcctccttct tctgccgtct
    26401 tcgctcgcgt cgatgctgtt cccggcgctg ttctccatcc cggacgccgc ggcgtatacg
    26461 gcgcgggtca cgctgcgtgg ggtcgcaatg ctcgcgctgc tcgcgggcgt actggcggcc
    26521 gtcgcgccgt tcgtgattcc gcggctgttc gggcccgcct ttgctccggc ggtgcggccc
    26581 tgcctcctcc tgcttccggg cgccgtgctc ctcggagggc agacgctcct ttcgcagttc
    26641 ttcatgcgcc agggagtccc gttgttcatg tcgggcttct gggcgatcgg gcttgccgtg
    26701 aacttcggtc tcaatctgtt tctgatcccg ggctggggca tgtccggggc ggcggttgcg
    26761 agctcggttg cctacacgat cgtcgcggcg ctcatggccg cgcgctttct caggttttgg
    26821 cgcggcgagg cggccaagga agcgggcgcc tgatggacgc ggtcgtctcc gtgtacccgc
    26881 cggcgggatc cacagggacg caggaggaac tcctggccct ctcgatgctg cggtctctcg
    26941 gcgcgtctgc ggcgctcgga acgcttcgga agcgccgcta tgcgcgtgcc gtgtttcgcc
    27001 tcggcgaggg ggatccgccc gagatcgagg ccgttgcgca tgtcgtcctc ggcgtgatgc
    27061 gcgcggattc gaaggggatc gagcgggccg gaggcgcgtt cgctccgctg acgatcccgg
    27121 gactgcttcg ggccttcgcg ggcctcgtgg tggccggcgt gctcgggatg gcggcggcgg
    27181 tcgcgaacgc ctgggacgtg cgctcgtcgc ggaagcccgg gggtcggcgc ctcgggccgg
    27241 gacggaccgt cgcgtatctg cgggcgagct tcggccttcc tcgggtcggc gggtcggtgg
    27301 gccacacgag cggcgtcgtg aacgcgctcg ctcaaaccgg cctcgaggtg cgcgtgttcg
    27361 cgtgcgcgga gccggtgggg ttgaagggcc gcctctcgtt caccgccgtt ccccttccgc
    27421 gctcggcgac ttatccgcac gagctgaacg cccatcgctt cgggcgccgc ttcttccgtg
    27481 cgtgtgcgcg ctcgctgtcg ccccgggccg tccggttcct ctaccagcgc tacgcgatca
    27541 acgacctgag cggcgtgcgt ctcgcgcgcc gcctgcaccg tccgctcgtt ctcgagtaca
    27601 acgggtccga ggtgtgggcc cagaagaact ggggtcgtgc ccttttcctg cccggcctcg
    27661 cgcggcgaat cgaggttgcc tgcctgcgag agtcggacct cgtggtgacg gtttcggagc
    27721 cgcttgagcg cgagcttctc gaccttgggc ttcccccgca ccgcgtgctc ttttatccaa
    27781 actgcgtcga cgcggacgtt ttcgacccgg cgcggttcga cgccgatgcc cggctccgga
    27841 cgcgcgcgcg tttcggcctc gatcccggag atttcgtcat cacgttcgtc ggcacgttcg
    27901 ggcgctggca tggcgccgaa gtgctggcgc gcgcgatccg gcttctgccc gagatcgtgg
    27961 cgggccggcg gctgcgtttc ctgttcgtgg gcgacggcgt caccgcgccc gagacgcgcg
    28021 aggtcctttc cgcggagcgc gtctcgggcc gcgcggtgtt cgcgggcccc aggcctcagg
    28081 cggagacccc ggaaatcctc gcggcgtcgg acacgtttgc gtcgccccac gttccgaatc
    28141 cggacggcac ggcgttcttc ggcagcccga cgaagctctt cgagtacatg gcgatggcgc
    28201 gtccgatcgt ggcctcggac ctcgatcaga tcggacggat tctccgaaac tggcagccgg
    28261 ggagcgtccc cgcaggcgct tcgaaggcgc cgctcgcgat cctcgtcgag cccggcgatc
    28321 cggggtcgct cgcggagggc ctacagcgcg ccgcgtcgct cgatcgggat gaggcgcgga
    28381 ggctcggcga agccgcgaga gccgcgatcc tccggtcgtt ccgctgggag gcgtgcgtcg
    28441 aggcggtcgt cgcaaggctc gccgccatcg atcgggagcg tgacgcgtga cgcccggcgg
    28501 gacggagcgt acgcggtgga cggacccgca gcggctcctc gtgaccctgc tcttcacgta
    28561 tctccctttt gcgatgtccg gccacctgct cgtcaaccac gttctgggcg gcccctggta
    28621 ctggtactcg gcgttcctct atcccgaagg cgttctgacg gtcgtcgttt gcgtcgtgac
    28681 tctttgcagg gccggggagc gcgagcgact gttcgaccgg ctctccgggc ccgggggcct
    28741 ttggtggatt cttggagtcg cgggcgcgtt cgtcgtctgg cttgccgcca gcgcgatact
    28801 tcagcgggcg ccgaccgggt tctggatcga acgcctcttg gcgggctggg tccttccgat
    28861 ggccctcgcg ctcgcactgc tcgcgcgcgc ggatggggtt tcggacgcct gggcgggctt
    28921 gcggttaggg ctcgcggcct tcgttccgtt cgccctcgcg ttgtacctct tcagcttcgg
    28981 aatcccgtcg tcgttccggg agctcgtggt cctgaaccgg acgtggaaga tggtgagcgg
    29041 ggtgcgcggc aaggtctact tcggcgagct gaccctcggc ggaatcaacg acgtcgccgt
    29101 cttcgtggcc gccgctttgg cgctcgcggg cggcctctgg ctcgtcgacc gaaggtcgcg
    29161 cgcgagccgc cgtgttctgt gggcgtggtt cgtggcgggg ctcgtcctcg agctgctgtg
    29221 ctactcgcga ggtgcgatcg tgtccctcgc cctcgtcgcg gctttctacg cggcggcctc
    29281 ctggcgattc gttcggctcc gtgcgcttcg ggtccccgca gcgctcctca ccgcgctcgt
    29341 ggcggttgcg cttgccctcc ccggcgcctg gccctactgg tccaatcagc tccgtgtcgg
    29401 cccgggctca accgctcgcg tgcgcctgac gctgtggcgc cggatcctga cggtgaacgc
    29461 cgaaacggcg acgttcggcg ggcagctccc ggcaccgccg ccgccgaaac cgactccgtc
    29521 ggtcgccccg ccgccgccag cggaaccgcc ggcggctcgt gccgcggggg cgggaagtgc
    29581 gacggccgcc ccggtcgtcg aagaggcgcg agagcgcatc gcgcggttca cgtcggcgaa
    29641 cggtcgtcag cgggtgctcg gtttcggtct cgggaactac ggcctcctgc aggggatgac
    29701 cgaggatgcg tgcacgcaca gcatgtttct cgacgcgctg gctcacggcg gtcttcccgg
    29761 ggccgttttc ttcgcggcct tctggtgcct cggcctcggc ctcgcggccc gggccgtgct
    29821 cctgcggccc gtcccttcga tcgggacgcc ggtcccggct tcgatctcgg tcgcagcggc
    29881 gctgtgtgtc ttgacgttcg cgggcgggac ggtgagtttc cagttctgga acctcgggac
    29941 gatgctgaac ggggccttcg tctggctgct cgtgatcaca tccgcagcga ccttccgcga
    30001 ggcgcaggac cggaaagggc aacgctggaa ggcggaaacg gtcgagcagt ggggtgcgga
    30061 cccatgcggc ggcgtcgagg cctcggaatt cggaaccccc gagtacttca cggccgtgac
    30121 gaaggaacgc tacgagatct acgcgccgtg gcttcccgca gccgcgggtt tctcccgggc
    30181 ggcgggcgtg cgactgctcg aggtcggctt cggcctggga accgaccttg tttccttttc
    30241 aagaggcggt gcgagcgcgt tcggcgtcga ccttacaccg ctccacgttt tcgccacgag
    30301 gcgccgcttc ctccagcagg ggcttccggt ccgcgtcgtg cgcggcgacg cggaacgcct
    30361 tccgttcaag gccggtgcct tcgacagggt ctattcgttc ggggtgcttc accacacgcc
    30421 gggaatcgaa acggcgcttg ccgagattca ccgcgtcctg acggcgggcg gcggcctgac
    30481 tctcggcctc taccaccggg acagtctcta ttgggcctat cagctgctcg tgcggggaat
    30541 cctcaaggcc ggattcctcc gccacggata tcgtcgaacg atggccggtg tggagaagtc
    30601 ctcgagaccg gacgcggaaa cgctcgtgcg cgcctattcg cagaaggacg tccgcctcct
    30661 gcttcgcgct ttccggggcg tccggctctc ggtggaacac ttctccttcg atcagggagg
    30721 gcgaatcggc ctcgtcctgg ggcgcatcct gcggccgttc gaggatcgcc ttgcgaggaa
    30781 gatcggctgg tacgtcatgg cggacgcccg gaagcaagac ggtggcgttt ccgcggagcg
    30841 cgcgtcgtga agatcctcca cgtcgtcggc gcacggccga acttcatgaa gatcgcgccc
    30901 gtcatggagg cgttgtcgcg ccgctcgggc gtcgtgcagc ggctcgtgca cacgggccag
    30961 cactacgacg agaagatggc ggggatcttc ttcgagcagc tcggcctccc gcggcccgac
    31021 cgcgacctcg aggtcgggag cggaacgcag gcggagcaga ccgccgccgt gatggtgaag
    31081 ttcgagaagg tgctcgggga agagcgcccg gacgtcgtcg tcgtcgtggg agacgtgaac
    31141 tccacgctcg cggcgaccct cgttgccgcg aaggcgggca ttcccgtcgc gcacgtcgag
    31201 gccgggctcc gctcgggcga cctctcgatg cccgaggaga tcaaccggct cgtgacggac
    31261 cgcctcgccg acatcctcct gacgccgtcg cgcgacgcgg acgacaacct gaagaaggaa
    31321 ggggtcgcgc ccgggcggat ccacttcgtg ggcaacgtca tgatcgacac gctcctgcgg
    31381 caccgcgcgc gcgcgccgtg ggacgacgtg agcgggcggc tgtccctgag ggaccgcggc
    31441 tactccgtcc tgacgctcca ccgcccggcc aacgtggacg cggcggacgc tctcgcgcgg
    31501 atcctctcga ggatcgaacc cgtcgccgcg gagtacccgg tcgtgtttcc ggtacatccc
    31561 cggacggccc ggcggatgac ggagagcggg atcgagccgc ccgccggctc ggtgcggctc
    31621 gtcgagccgc tcggctacct cgagttcctc gcgctcatgg accacgccgc gctcgtgctc
    31681 acggactccg ggggaatcca ggaagagacg acggtcctcg gcgttccctg cttcacgctc
    31741 cgcgacaata cggagcggcc ggtgacgatc acggagggga cgaacacgct ggtgggggcg
    31801 gacgccgccg gcctcgctgg tgcgtacgcc gcgttgcgcg ccggcacgcg gcgcgcagcg
    31861 aggatgccgg agctctggga cggccgcgcg ggtgagcgca tcgcggacgt gctgctcggc
    31921 gccgtgtctc gcggctcgag cctgtagaga taccagaaca cgtcgcgggg cgccgccggc
    31981 gggccgatgt agcgagtccg gtgaacgaga gtgaaggcgc agcggcgtgt ctcgagccgc
    32041 gcgccctcgt ggagatcggc ggccgcgagc acggcgaggt cgctggccct cagctgcggc
    32101 tggttggcgt agaggttctc ctcgaccatg acgtgcgtgg ccccggcgtc gagcaggaag
    32161 tcgacgaggc ggccttcgcg caggtacggc aggacccggg tgtcgaggat tccgtcgagg
    32221 ctcaggacgc ggcgctcgcc gtagtaaccg agctggtcga cttcctttgc cccgatcacc
    32281 gcgtcgggag gcaggtgatc gtggacccac gccgccatct cgcggcgggc gggaaggtcc
    32341 cacggcacga gggtgccacg caggtaggac cgggtgtcgc ggaattcccc gacgtggacg
    32401 cgaagatcgg gcacgagcgc gaggagaaag agcgccggag cggcgacgcg caggcgggcc
    32461 ggaatccagg cgaccgacgc cccggcggcc acggcaacgg cggcgaagcc cggcacgaag
    32521 taccgctggc cgtaggtcgt cggcatcacg acggtataga gcgcggtcga gaccgcgagc
    32581 caggcgccga gagcgagggc caaggggcgg gtttcccttc tgcggagcgc ggctgcaagg
    32641 cctgcaagag cgagcagacc gcaggcggcg tagggaccct cgagaaggcg aacgtattcg
    32701 aggttcacga agaaggggcc gtggcgagtc gcccactgcc gagcgaactc gatccgggcc
    32761 gtcgaggaag ggagcggaga gccgaagttc acgaggagcc aagcggcata cggggcgacg
    32821 acggcagcgg ccgtgcccag tgccgcgatg aggtttccgg gagccgactt gcggttccat
    32881 gccttccacg cgaggaccgc agccgccggc accgccagga gaatgccctc gggccggcag
    32941 agggcgccga ggccgaggag ggcgccgctg aggactgcgc gtcggggcgt tcccgcggac
    33001 tcagtcgacg aaacgtcgag gaagatcgcg aggacgagga agacgaagaa ggccgtctcc
    33061 atggcgcgaa tcgtccagat ggcgaacgcg ggtgcgagag cgagggcgag ggccgcacac
    33121 ccgcccgcaa tagggtcccg ggccaggcgg cggccgaggg cggcggcagc cacgaggccg
    33181 agcgcggcga aaagaaggct gagggtcttc gagagcagga tcaggcgcgg gggcccgccg
    33241 aagaggtgga ggatccccag gcacatcgtc catgccgggc tcgagctgcc gcgggacggt
    33301 tctccggcgt tgaagtggaa gaactcgccg cctgcgaggc ggcgcgcgaa cgtgtagtgg
    33361 atgtacgtat cgccctgcag atccggccag agggcgagac actgaagggc gagaaccacg
    33421 accgcggccg cgacgagcag acggccggca atctcgttcc cggaagacgg caggttgctc
    33481 gtgaggaaac aatatcatcg cggcacaagg cgcccgttcg tcatgaacat cctcttcctc
    33541 tccgacaatt tcccgcccga gcgcaacgcg tcggcgacac gcgtccatga gcgcgcgtgc
    33601 tactgggtgc gggagggaca ccgcgtcacg gtgatcacgt gtgcgccgaa ttttccggac
    33661 gggaaggtgt ttccgggcta ccgcaaccgg ggctccgtcg aggacctcga cgggatccgc
    33721 gtcgtgaggg tccggacgtt cgtcagcgcg aacagggggt tcttcctccg gacggccgac
    33781 ttcgtgtcgt acatgctggc cgccgtcgtg gcgggactcc gggaggaaga gcccgacctc
    33841 gtcgcggcga cgtctccgca gttcttcgcc gcggtcgggg gctggatcct cgcgcgcctg
    33901 cgccgtgtgc cgttcgtctt cgagctcggc gacctgtggc ccgcctcgat cgaggcggtc
    33961 ggcgcgtcgc gcagccgcgt gctgctttcg ttgctcgaga gcgtcgagct gttcctctac
    34021 cgccagtcgg cggcggtcgc ggcgctgact caccgctttc gcgaagatct catccggcgc
    34081 ggaatccccg gcgagcgaat cgccgtcgtc cgcaacggcg tggacctgcc ccgttacgcg
    34141 ccccgcccgc gggacgtgtc gctctcccgc gcatggggcc tcgacgggcg cttcgtcgtc
    34201 ggctacctcg ggacgcacgg aatggcccac ggactcgcga acgtgctcgc cgcggccgac
    34261 gggcttcgcg gatcggacgc gacgttcctc ttcgtcggag cgggggcgga gcgcgatgcg
    34321 ctcgtgcagg atgcggcgcg gcgcggcctc gacaacgtcg tcttcgcgcc cgcgcaggac
    34381 aagggcgaaa tgccgcgcgt ctggagtctc tgcgacgtcg ccctcgtcca cctgaaggac
    34441 gaccccgtct tcgagacggt catcccttcg aagatcttcg aggcgatggg catgggcctc
    34501 ccggtcctcc tcgtgtcgcc tcctggggag gcgcgcgaaa tcgtcgaggc ggagggagcc
    34561 ggaatctggg tcccggcggg caggcccgag gcgctggccg acgcggtgaa ccttcttcgc
    34621 accgacgaag acctccggcg ccgtctcgcg gcggcgagcc tggcggccgc accgcgtcac
    34681 agccggaagc gacaggccga cgaaatgctc gaggtgttct cccgcgttct cgcggaggtc
    34741 tgacgcccta ccggcgggcg cgctcgacgg ccttcaccgc ggcgcctgcc acggcgtaga
    34801 gaagggcgag agcggcgagg atcgtgacgc gcacggggcc cgacccggtc gcgtaggcga
    34861 gcgcggcggc gcccgagact cccatcagag cgatctccca acgcaggacg cgggcgtggg
    34921 agaggcccga caggacgagc cgctggtaat ggtgcgaccg gtggggagtc cacacacgct
    34981 cgccggcgag ggcgcggcgg acgagcgtga ccgtggcgtc gacgatgaac ggcgcgaaca
    35041 cgaggcacga ggcgccgagg tcgacgcggc cagccgcgga ggcgcgcagc gcgagggcgg
    35101 cgacgaggaa cccgacgggg atcgcgccga cgtccccgag gaagatgcgc gcgggaggac
    35161 gattctggat gagaaaaccc gccgcggccg ccgagaggaa gaacgagacg gccgccccgc
    35221 cgccgtcacc gcccctccag caggcgaccc cgaggaacgc gcccccgatg gccgtcatcg
    35281 atccggcgag cccgtccatc ccgtccatga agttgtagag attggtcagc cagaggattc
    35341 cgaggaagct cagcggcgcc gcgaacggtc cgagggcgac gtcgccggcg agcggcaacc
    35401 acacgcgttg cgcggcgagc ccggcgcccc acacgagggc agccgccgcg agcgcctgcg
    35461 cggcgaggcg cacggaaacc ggcaggccac ggcggtcgtc cgcgaaagag agcagcgcga
    35521 gcagggtggt cgcggctccg atccaggcga aggacgtgtc ggccgtcgca ccggcgacgg
    35581 cgagcgccgc ggcgacgccg aggaggatcg cgacgccccc cgtccgcggc gtgggctgcg
    35641 cgtgcagcga acgcgcgttg ggaacgtcgc gcggccagaa cgacgaccgt gaacgtgcga
    35701 gaaggacgga gagaagggcg gaggcgaaga acgcgacccc cgcgccaagt gcgacgccgg
    35761 cggcggtcac ggaccggacc ttctgatccg gacgatctcc ggaagcgcgg cctgcagggt
    35821 cgtgacgggc cggaagccga gctcccgccg gatacgctcg ttccggtagc acgccgagcc
    35881 gagcagcttt tcgagcgccg cgcggccgaa cggaacgcgc cgccccgtgg cgcccgctgc
    35941 ggcgtccccg aacgccgctg ccgcccggag agcggtcagc ggaacgctcc agcggaccgg
    36001 ccgtttcccg agggcctcgc gcatcgcgtc gtagatcccg cgcgacgaat agtcgacacc
    36061 gtcggtgacg aggtacgtgc gaccggcggc ggcgggcgcc gccgccgaca ggcggaacgc
    36121 ctgcacgacg tcaccgaccg acgcgaggct gcgccggttc gcgacacctg ggagcggagg
    36181 gaagaacccg ctctcgatcg cgtcgagcat cgagaggaga ttgcctttca tccgggggcc
    36241 gtagacgagc gggagtcgaa gaaccgtggc gcgcatccgg gaacgcgccc ccgcctcgag
    36301 gacgagcccc tcggcctcga gcttcgagcg gccgtacggg ctccggggcg aggccggcgc
    36361 ctcctcgtcg agacactcct cgcccccttc tcccatggcc ttgacgctgc tcgcgaagac
    36421 gaacgaggcc gtgccggcct ccagggctgc ctccaggatg cttcgggtcc cgtcgacgtt
    36481 ggcggcgcgg aacgcgtcgg atcgtgttgc ggcgtcgagg tcgtgtgcct tgccggcgag
    36541 atgaaagaca ctgtcgcatc cggcgacggc gcggcgaagc gcaagagggt ccgagaggtc
    36601 tgccgttatg gactccgctc cggatggcaa ggcttgaggc ggcgtgcgga acatggcgcg
    36661 gacgggttcg ccgcccgcgg cgaggctctc gacgagggcc cgcccgatga agccgccagc
    36721 tcccgtcacg aggtgcacgc ccgatgctcg ccgcgcgccg ccctcggctc cagcccattc
    36781 ggggccgcgc tcagggcgcc gcgcgag
//
LOCUS       DAOMFY010000012        34606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_396_length_34606_cov_9.040028, whole genome shotgun sequence.
ACCESSION   DAOMFY010000012 DAOMFY010000000
VERSION     DAOMFY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 34606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34606
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_396_length_34606_cov_9.040028"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PLB01_02190"
     CDS             complement(<1..1446)
                     /locus_tag="PLB01_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQQ76137.1"
                     /translation="MRTLSTRALSTPEPLVRAFARRVQQEGALDLTQGDYKNSDFAPH
                     PEVVRSAQRITRNTVHSYGPAVGRMDVRSEIADFFNRDGLRDYPASDVRFLPDEILFT
                     PGTRAGLAIVLEVLGDDGSGVVVPRPSWEYDWFIERAGKRVVELPTDAPAFLPDPAAL
                     DRILAKGGVSSVILNNPHNPTGRVYPRALVEEIVRVAVKRRVFVLYDSVYQRLDYVDS
                     FVNPAFADPEWRDWVVTMSGLSKMDMFGASTGARACWLVLSDEIRSNGVRAREILANL
                     SAWLVATPSTLAQDWALAALQSPLAALRRPSPYMRERRDFMLHAADALAPLGVERTDF
                     GGTFYAPLAFPGLVGASFDRLRNGVKERAVVKTSVDAFELLLTGGVGGIPFAAFAGPD
                     AARYGTWQRLSYGSKDVPELAVFMDRVKTRIEAAARSGSSAPAPAPGRTVEASVWESA
                     CTEGGYDALDAWASDNVAFPAAAYTTYIDELY"
     gene            complement(1487..3343)
                     /locus_tag="PLB01_02195"
     CDS             complement(1487..3343)
                     /locus_tag="PLB01_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ76138.1"
                     /translation="MVFRGLSRLSLAPGLCSLSLLAAEPAPLPAAAPIAADVVVSAEA
                     APEPSVSLGTAATVIDAAEIARSKAATVLDLLRTVPGLDVVQSGGVGGVTSLFLRGTS
                     STQTLVLLDGVALNSPYFGATDLSAISTANVERVEVVRGPFSALYGSEAIGGVVQIFT
                     RRAADAGASGHASFALGNAGGKEGLGEFGFSEGVLSGTLGFRRTLSWGDQQNESFAGT
                     SLSGALTARLGEGATAGVVVRRETGRTGIPSDGAAPTPQRSTSADTTTVEVPFSYSLS
                     KKFSLDLSVRYTRDQPEYADPADPFFTSSTTDARRAGGRVAVSGTFGAHRLAAGADYE
                     RTQVSSESNFGVALDGATSRTLALFVEDRIALAGEKLVATAGVRWDDHSAFGGAVSPR
                     VTLAWRAAPSLKLRAAAGSAFRAPSLGELYYPYSGNPNLEPERSTGWEIGAEWTILDG
                     VVGEATGFWNDIRDLIQYDAQTYSNANIGHARTRGVEVAVRTAVGERAFLRASYTVLD
                     AKDLDADAPLIRRPRNRASLTAGSGFKAGGSFSVTAIFVGARTDRDAADFTTVVESPS
                     YLRVDAAITLPPLVWSLSPWLRVTNAFDRAYAEVNGFPAPGRRFLAGIEASF"
     regulatory      complement(3451..3664)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3711..7460)
                     /locus_tag="PLB01_02200"
     CDS             complement(3711..7460)
                     /locus_tag="PLB01_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQQ76139.1"
                     /translation="MSLLLAVLAAVALWFFATFVPDRRRMAIEGWKQELSLRADLRRD
                     LIERRILGASEELTFVAAFPSVRELLKPGLSDEAAGAEAVHLGGILADYRRIHQVRSV
                     WILDPSGRIRASGEGAAPGAAGLALAADVIHSGARGVALGNEPDGVAGLLSAVPVAGD
                     AAGSGRAPILGAVLSVGDASTFLFDLLGQPLPDPSSESLLVGLHGKDLVFLSPLRFRP
                     DPPLSFRLPLATPGLAARAALTERESFQAFVDYRGVRVLAAVRRLEAAPWGLVVKVDE
                     NAALAPFRRDVLQRGFAWGALLLAFFGTAVALGRWRESSHEARLARSEARFMDLFEQA
                     NDAVFIIGQDRRVRAANRAAEETYGWSREELAGRDVADFRPAGQQEQAIERFAAAEFG
                     HRLVFESEHVRADGSRFPAEVSTRRVDGPDGPVHLSIVRDITARKAAEGRIRKLNRLL
                     RTISEVNQLLVRATDEGSLLGEVCRILVAHGGYALAWIGRADRETMRVEPVASAGDEV
                     GYLRRIEVRWDDTPEGRGPLGTAIRDGHAVIVSDVQTDPLVDVWRREMLRHGFKSLAA
                     IPIRRGGRVTKGLVVYAAEKEIIDEEESALLAELAGDLSFALDALDAREALRLSNAEL
                     RKLWRAVEQTPATVVITDLEGRIEYVNPEFTEATGYSADEAIGQNPRFLKSGLTPPEV
                     YRAIWEAVTTGRVWQGELCNRRKDGSLFWERASISPVREEGGTVTHYVAVKEDITERK
                     RAAEALAKSEAYYRSIIENSLDIIAVLDPDGVVGFASPSVADTLGYRPEELVSRSIFE
                     LIHPEDRAMVMSRLHELVFAGAAPRKAEVRFAHRDGSWCPLSVVGNRLPDETGMSGVV
                     VNARDLTERLHLEAQIRQAQKMEAVGRLAGGVAHDFNNLLTVIQGYGELLWETLEGDP
                     DNRESVGEIVKAAERAARLTRQLLAFSRRQVLETRVLDLSSIVTDVEKMLKRLLGEDI
                     EIVVVMPRVVGNVKADPGQMEQILLNLAVNARDAMPGGGRLTLALADADLDAPLAAAA
                     DLVPAGRYVVLSVTDDGCGMDHETLSHVFEPFYTTKERGKGTGLGLATVYGIVRQTGG
                     YVAVETAPGAGTTFRIYLPRCDEKTTSGVHPAVVSRLGAETVLVVEDEPAVQALAAAI
                     LRRRGYTVLVAESGSKALDLVESDPRPIHLILTDLIMPGMNGRELATRMRALRPSIKV
                     VFMSGYAADAAPHLAEIGASGFLPKPFSENALVTKVREVLDTPPA"
     gene            complement(7541..7789)
                     /locus_tag="PLB01_02205"
     CDS             complement(7541..7789)
                     /locus_tag="PLB01_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76140.1"
                     /translation="MDESEALEPFWNRVGGAAIGWCAGLLGVLDLAWGIGQRLRRLRA
                     DADARGESRFRTLFEQASDGPRRRSGLKAGASGAPPKA"
     gene            complement(8203..9693)
                     /locus_tag="PLB01_02210"
     CDS             complement(8203..9693)
                     /locus_tag="PLB01_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQQ76141.1"
                     /translation="MSQTLIAAHPADGAESADKKTFEAAMAADDARPRKKLVEGQVVN
                     GTIAGITPDVVLVSIGGKSEAIMDLKELDGEKVGDRIEAVVIKAAPDVRISRKLAIGH
                     KTKAELRAAAEAKIPVAGKVSARNKGGFDVLIGGAHGLRAFCPVSQIDLGRHDEAGLA
                     AFVGKNFDFRVIEYSEDGRRVVVSRVALLKEDNEAKAAETRAKIELGSVMTGRVRSLT
                     DFGAFVDLGGVDGLVHVTEISRRRVAHPKDVLTVGQEVTVKVTKLAGEGKRISLSMKE
                     LEADPWATLAERFAPGASFTGAVVRHADFGLFVEVEPGIDGLVHVSALPPGVSAKDPQ
                     VAVGQTVQGWVKEVDPAHRRLSLSLREVATGDPWAGIGERYPEGSVVKGEVESVAVFG
                     VFVRLEAGLTGLIPNGETGVPPGTVAKHFASGQKVEVKVIGLDTQRKRISLSASGAKA
                     EADRGDLRSYREETARREKAAAPSVSIFGASLLAALNNPKSKQKKS"
     gene            9945..11930
                     /locus_tag="PLB01_02215"
     CDS             9945..11930
                     /locus_tag="PLB01_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQQ76142.1"
                     /translation="MHLGRILDDKAPNYLKQGLGWSYHAPSAGHDGIQVALGVTFRPN
                     VDYLFPYYRDLTTCVAAGLSADEILLNGMSKKQDVASGGRHMSNHFAKPSIHIQNVSS
                     CVSNHAQHAAGLGRAIRTYGADSVVFCSQGESSTSEGYFYEAVNGASREKLPVVFVVQ
                     DNGYGISVPKSDQTANLYASDNFAGFLNVKIVHCDGKDLFDSLRALREAVAYVKSGAG
                     CALVHADCVRINSHSNSDRHELYRTPEELAAAKALDPLPRLRRTLVEGGAFTEDEIAE
                     IEASNVRLYDEAADRCRKAPDPDPASIFDFVSPEPWVPAGDGLATVARTPAAETAPEL
                     TLIQALNGTLKEMFRENPDTYIWGQDVAYKDKGGIFNVTKGMQQEFGAKRVFNGPIAE
                     DFILGTADGFSRFSDKIRVVVEGAEFADYFWPAAEQMVEMSHEYWRTNGQFVPNVTVR
                     IASGGYIGGGLYHSQNIEGWLTTLPGIRVVVPAFADDAAGLLRTAIRSRGITVYLEPK
                     YLYNAREARTHVPADFAVPFGKARLRRTGKDLTIVAFGTPVHFALDAAAALAKEAKEV
                     EVLDLRSLVPLDFDSIAASVKKTSRVLVVHEDKVHGGFGGEIAARIASELFEHLDAPV
                     DRVGSTFTPVGFNRILERAILPDATRVLAAARKTLAY"
     gene            12214..12654
                     /locus_tag="PLB01_02220"
     CDS             12214..12654
                     /locus_tag="PLB01_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76143.1"
                     /translation="MCVPVAAGRPYVWGFSMYFPDPNRVAGLSEYIAIYDGPGCTGAN
                     IGGYSLPIIGVPGIWFGGTFGLFPTPAGARSLRAGFGGLGGAINQATAYVDDVFVAPA
                     GTVPPIEPVPEDVPFLSTPGLLALGAALALAAARLLGAALPSSA"
     gene            12689..13333
                     /locus_tag="PLB01_02225"
     CDS             12689..13333
                     /locus_tag="PLB01_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76144.1"
                     /translation="MRFCLVAAAVVVLTTPARSQNLVADPSFRQGLGAWSDVYVIGAY
                     TMTWVPSPSVRPGSGSALLSFDAPARGAWSVCVPVQGGLQYDYGFSIYYPDAARTIGL
                     NEAYGVYSGPGCTGTDLGGYVLPIVPAQLNTWITRAPYRFTTPPAAQSVIITFMALGV
                     GGAKATAYVDDIYLGQAGTIPPIDPPPAIPSLATPGLLALGAALALAAARILRA"
     gene            13465..15768
                     /gene="ccsA"
                     /locus_tag="PLB01_02230"
     CDS             13465..15768
                     /gene="ccsA"
                     /locus_tag="PLB01_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQQ76145.1"
                     /translation="MLEALLKKLFSNLASLKLTVVLVLLIGVVLSAGTIVESLHGTKA
                     AKSVYEATWFYVLLAAFAFNVFAALIDRWPQTRWRIGFAITHLSMLLILLGSVVTVVA
                     KVEGQMPIDEGTSSNRVLRGERGELPPIVLPFSLHLDAFELDTYPGTRMPAQYRSRLK
                     IVSGTSAQPTVIEMNKPLSYGGFQFFQSSYHIDEGHRTTILSVSRDPGQPIVFVGYTL
                     LVLGMCVVFATRLLQAKQAERQSPARVPLTVGILALAALLSPSARAVEVPDAATVEKL
                     RALPVQHDGRTMPFDTQAREALRSVTGSSSWPGLDHVAMVAGWTADPNGWENAPIVRV
                     GGSAVAQAAGLPSGTRYASFAQLVSSQAFRAAATAAHRAEQEERKPTSLEKDLLKLEG
                     RLSTLYGYFQAQQIKPIPSPDPKGAWRPLPNNRSAAAIAGVAAQVRAAGAPAHYPDDK
                     AISRELLYNRMRPSRIAWLFLLPAAVAAGYVVAGRTKLRPLVAALLLAGFAAMTWGIA
                     LRWQIAGRVPASNMYESMLFLGWGVGLFGVVSLLLRQPLLVANAAGMGALAMMLVDLL
                     PMDPFIHPMMPVLTGTPWLAIHVPIIVVSYSVLAMATGLAHLVVGVEIFAPARRDLSR
                     RWSQLLYYYLHVGSILLIAGILTGSIWAASSWGRYWGWDPKEVWSLVAFLAYMAILHA
                     RFDEQIRDFGVAVSSIAAFWTILMTYLGVNYVLAAGLHAYGFGSSNLVSIMTVIALVE
                     IAFLLWGAKARRGRGGPAGLTLPVAGR"
     gene            15785..17320
                     /locus_tag="PLB01_02235"
     CDS             15785..17320
                     /locus_tag="PLB01_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ76146.1"
                     /translation="MASLIPSVPLPWFRVALGMNPARLRFWGVVVPIHAVAFTALYLG
                     NLKLLDAAYSEAGVAAARRQVEQLVRELPTMMPSIPGERSPHLFGHLISMHQPIRLRL
                     YSVGASLIWPPYHPADADEAARVQRLMHDNAVRDDVWLEKRDGRQWLRGVVRVNTGED
                     CRPCHAAGKTLGAASVTIDFTDELNELHAILRRRVGWLFGIWIVLVGGVAFVAQKTIR
                     RSLSRLESELDHAAAGRAPSEPAGDLPLDAASAAFHGRLRDFLRRQRAREVEMVSRLE
                     RVDQLATLGELSAGLAHEIKNPLAGIQGALEVLKHDTADDATGRLYDEMLGELKRVNT
                     ILQRLLESGRPAPLRLSRTNLERLIAETVTLMRPALSRKKVDLSAAVEDDLPEIRADA
                     AKVRQVLVNLVQNAAEAMGESGGSVRVRAGKVPDGDGVVLAVEDDGPGIPREGLDHLF
                     EPFYTTKFTGTGLGLTISKSLVEQHGGHMEFVSEPGRGTTFFVFLPSGDAADDASPLE
                     KAG"
     gene            17322..18686
                     /locus_tag="PLB01_02240"
     CDS             17322..18686
                     /locus_tag="PLB01_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ76147.1"
                     /translation="MALALIVEDESLLRWALSRQLERAGHKVHAAPDLAAAAEHITAH
                     RPDVILLDLGLPDGHGLDFFEANRERMAESKVIVMTALSQVEEAVRAMKLGAIEFLTK
                     PVDEADLVALVNRSLAVRNDQLEAQAMRRSRELRLSKQAIGESAPFKGVLDVIDQVAA
                     SDIPSILIQGESGSGKNVLARRVHSLSARQDKPILEVSCATIPENLLESELFGHEKGA
                     FTDAKGLKRGVFELAEGGTVVLDEIGELRLDMQAKLLHFLEERRFRRVGGTREIQVDI
                     RVVGLTNRDLQAMVREKTFRTDLFYRLNVFPVAVPPLRERPEDVLPLARHFLTNARSN
                     LGTRFSGFDAEAERLLVRYPWPGNVRELRNVVERALVVERGEKITARSLMLDAVPAEE
                     GASGAAVLPPGIVPLETLEREMILRAMLATGGNTTRAAEPLGISRDQLRYRLKKLGIR
                     ESGD"
     gene            18723..19133
                     /locus_tag="PLB01_02245"
     CDS             18723..19133
                     /locus_tag="PLB01_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76148.1"
                     /translation="MGTPLRIFVLDESRITALMVERVAPHGTVVAAFTSLAEAACAFS
                     AAPPDAAVVSLTGPHAPWREFRDLCASRRPLVPILYESCLYASAEEAGLEPTSRPVLF
                     LHTPAPFAEFQRAVAELLAAAIRVKSSPNLLASA"
     gene            19502..22747
                     /locus_tag="PLB01_02250"
     CDS             19502..22747
                     /locus_tag="PLB01_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ76149.1"
                     /translation="MADGRRTLGFLCVAGGILFFAAFASSSAFAAGKPAGKACIDCHA
                     PVAKQLSKRFVHAPFKDEKGCESCHKRHGVVGTLALKDEQPALCVACHGKEGEAAKAK
                     VVHAPLAKGSCTACHAPHASDAKGLLKAAGNDACFSCHKKDAFAKKFVHAPAAASCLT
                     CHNPHGGAEKHLLVKSEKDLCASCHKAGGSAKPGKHAGLNVDSVACTSCHSPHAADEK
                     SLMRAVVHAPASECTSCHVAGPGKPALAKAEPALCADCHSDKTDDLKKPVVHEPFKDT
                     CTTCHAPHASDRKALLASSQKDLCGGCHDLSKELTDKAVKSTHKPAAEGSCSSCHEPH
                     AGKAKHLVKAEGAALCATCHEKTKAWGAKKNVHTPFAGGECAACHVPHGGPNPKLLAK
                     AGGETCFACHEDQKKAFARTFAHAPVREGDCAKCHDPHGSDLAKYLVKPPDQLCATCH
                     AQTIARAPSADGKNGTPHPPFAGGECATCHDPHATSRTRLLKDVASKLCEGCHSDVVD
                     GVAKQKKAGSGSVHQPVVAGTCESCHDAHGSKQKGYLTAAPRALCLKCHGALEARLKK
                     AKAVVHKPVADGKCLDCHGGHSSASPDLLVKPVPAVCATCHDLKSADLLKKHGGFSLE
                     KTNCSSCHDPHVSQKKGLFNPVFHPPFTDGDCASCHKPAAAGAPALAPAASLNAKGAA
                     LCTPCHGDFPDRFKKAWTHAPAKDGDCVACHSPHATKEKKLVAAPLKTLCTGCHDSVA
                     KDLAAKSVHPPVKDGLCAKCHDAHASDIKKGLVKAGSDLCFGCHAAERTLAAKAKTQH
                     APFAKGECGKCHDAHASANPAQLREPVATLCRKCHDVASPKVSAAHKGIPVAGADCAS
                     CHHPHASDQPKLVRNVQHQVLGSCARCHNTAGGKPKELVAKTTDLCFRCHGAVKVASQ
                     KPNAHKALQQGCVACHTPHAADAKGLPKGNERENCLSCHAQKKKQAETARSVHPLKVE
                     NGRCSICHTGHLSEFGSLLKTAPENLCKGCHKSHAEFAHPFGPGIVDPRTGRTMTCLS
                     CHDPHGTPFQATLVDNPQRALCVQCHKSDGASLGTGHGGTAKRAPSGRPTP"
     gene            22744..23778
                     /locus_tag="PLB01_02255"
     CDS             22744..23778
                     /locus_tag="PLB01_02255"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HQQ76150.1"
                     /translation="MSGRTRPVLFAILLALVSSATRGEEEKSEQIWLRPVALFDQHLY
                     EGALAEPRGIFYDAASKEVWVADTRNGRIGVFTPEGIPLFSFGNTDKLREPIRLAVAK
                     NGEVYVLGIERGRIERFSYRGEYLGPLSLPGVPAKPVFGTIAVDADGNLWVGENETGQ
                     VLGFSPSLKPLLRLGSSGDGEGQFLAIAGIAVSKDYVVVTDHTALAVQVFDRRGNFVK
                     GWGKHEMGREHFSLPEGVALDKQNRIIVADALRHEIKLFDLEGNFLDRFGGLGSKAGN
                     VSYPSDVAMDAGGHVFVAERGNNRIQVFVETAEPPLDARSKPQADRKEPVSPAERTRV
                     SPDSPKDSNR"
     gene            23807..24961
                     /locus_tag="PLB01_02260"
     CDS             23807..24961
                     /locus_tag="PLB01_02260"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ76151.1"
                     /translation="MKKTLIAVAALVAVIAFPAIAGEWHSGENNLCTDCHTMHFSQSH
                     NWDGTTPVSTTPQPNGNWLSATGPNAFLLKAPANQLCMSCHDGQTFAPDVVGANFNAS
                     PTNGRSAGAINDVALGAPYDTWKGHTLDSTATPPGFDPTKIGASATWYDPAGGLECIS
                     CHAQHGPVTAYRNLGPYALGGAATAARPTYVIATTNDTTKDVWINIAAPYTAGSGSAA
                     TFNSYYADANVSFNRNDATVGTNKTSNRMDTFCGACHGNFHGGPADTNIGATVAALDG
                     FVRHPTSQVTIGAAGAQGFGGHSALSRYTGATTKVNVYASDRVGYTDATPGCVTCHKA
                     HGNQNPFGLVFLDRNATSVGEEGGFGADDTDTSVKTGYRNLCGQCHSQGD"
     gene            25036..26094
                     /locus_tag="PLB01_02265"
     CDS             25036..26094
                     /locus_tag="PLB01_02265"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ76152.1"
                     /translation="MRTHPGFLKLFLAALVIVAAPARAGEYHYAETLLCSDCHAMHFS
                     MQHDWDGTTPVSATAQPNGDWLGATGPNQYLLKAQESQLCLSCHDGQTWAPDVYGANT
                     GTAYVRQAGAIPTGTAPYEQWKGHTLNVQATPPGGTSNIRLQCVSCHSSHGNVNYRNL
                     DGATPIISYARGTNNTTKDVFLRSFTLGAITTNYATQNVDFNEPGPNASAMGRFCRGC
                     HTDFHGGQYDSNMFDGVIGVGGWIRHPTAGANIGALGGGHSSTATLAAHAYRVQVMSP
                     TGTWGPQGATWGAPPGDLSPSCMSCHKAHGNQNPFGLIFLKGTGPELTEEGDAAGNTG
                     PMGQRLSALCGQCHVQAS"
     gene            26173..27087
                     /locus_tag="PLB01_02270"
     CDS             26173..27087
                     /locus_tag="PLB01_02270"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ76153.1"
                     /translation="MTKRLTTARRAGLLVTVAVLLAAAASWRASGGDWHNADQLACSD
                     CHTMHNSSNGQPMRYDNDPQPAPTLLRHSSSYALCTYCHDGSNANAPDVIGPVSYVAD
                     PQAGFFPQDVVTRTANPNGHDLAALNPELPPGGTTAMTLTCVSCHAPHGNANYRNLVT
                     DPAGTSANIAVAAYQAIKANGSNPAQVYVQSNVVPKSGMSDWCGACHGNFHGRTPSQE
                     GTASPWLRHPQDQTLSTSAHVDYAYWTGAVANRVPVQNPTSNTIPSSSDQVFCLSCHK
                     AHGSQNKAALIFADGATKLSTCEQCHNK"
     gene            27084..28478
                     /locus_tag="PLB01_02275"
     CDS             27084..28478
                     /locus_tag="PLB01_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ76154.1"
                     /translation="MKRTLAAAAFVLAAIPAAAGVYTATKHGSTTTGAQRLGTEPRGD
                     CAQCHDEHASRDGAPTGGPFPYALFAADDNALCATCHAGSAANGIYTGSAAYAGSSHS
                     TGNTIWPGPTPPSRPSSDAGKCVNCHDPHGRSDVSGLIPSMTVAREESLCLPCHDGSP
                     ATKNVSAQFSKTYRHPIATAGKHNEAEAGDPTKFAASPTNNRHSECADCHNPHAAASD
                     RFAPTAPSISNRLLGVGRVRVTNGAAGTKPVYTYLGPGDPSPAGEYQICFKCHSSWTT
                     QPGTATDLGVLLNLNNPSFHPVEAQGKNRNINANSFVNGWTWDKLTYCTDCHSSDDAT
                     VRGPHGSSWQYILKLSSIASPARRATVMASTELCFACHSYATYANNGSSNTVKGYSRF
                     NPPQLSQGHTYHVGSQRYSCYNCHASHGSATRPSLIVTGRNPGITSYTQTANGGTCTP
                     TCHGSESYTVNYAR"
     gene            28486..30648
                     /locus_tag="PLB01_02280"
     CDS             28486..30648
                     /locus_tag="PLB01_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76155.1"
                     /translation="MELALALAVLASARTAPAQQDLTGRAILSYQTLDADVLRTDGFH
                     QTYDLRLERAVSEAFRFRLSFRGEGNDGSTRLDDSAARDQKYRQYQPGAEVLWTFANV
                     QIQGNYDDYRTDTSTGDLADERHQRRVVGRMTWTPEKLPALTLYGEQRALTDDMARID
                     QKENTAFEALDFTWKGLTLGQNLRYQTLDDGGADFARKSTDLQGLARYENTFFGGKAA
                     VSANVLLGASRLDERASSGQPVRVPIRVLVGRALYAHDDTPQDGRDRPLQDLGALIDG
                     DFLKPAGVSLGPDGTSYQNLAADMGRFIPLDEFRVYVRDVSGAPVKTPGMVRFDAYRS
                     DDGLTWIPVAGGSRTSFDPARSAYVVAFASTNVRYLKVVSFGTNVLDTQVTEIEVYQL
                     QTFGAGEVKRTDIRLGSGSATVSGKPTEWLTLGYYGLFNTNTQTSTTRPELTTKDSDQ
                     IASLQVDPVKNVNLLARYERRISTQTDGFGQTLDAITATAHWAPLRTIDVTAEYQRSE
                     QDLAGRTSTSNAYHGHVYLKLLKSLDLSLDAGAAREVQSSDNHPASHVNFTGVSYAQL
                     TDDLKLTVNVSYSKTSYDGGTPEEIGTGLLLPASHDTRYWTELYYRPGQQLALTARFG
                     YASSDLYSGPTQGYRIEWFPFGSGSVSIGTIYEEDIDSVSNRRFRRVQLLPRWVLNKN
                     MTLDLNYTVMSSYLGSADGSAAISLTSRNFWATFTLAF"
     gene            30680..31258
                     /locus_tag="PLB01_02285"
     CDS             30680..31258
                     /locus_tag="PLB01_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HQQ76156.1"
                     /translation="MRLVKAASAALAALTLLALAACSSGSSAKYTHPNADLGAIKRVA
                     VLPFENLTQERSASDKVQKVFLSELLATEAFDVVEPGLVVKALKAERIESIDAVGPAE
                     AKRLGETLKAQGLFTGAVVDFAEGRSGNVAAPEVTIQLRLIEVQSGVTIWTASKTKSG
                     ASASARLFGVGGQTLTEAARELMRGQLKGLVK"
     gene            31255..32415
                     /locus_tag="PLB01_02290"
     CDS             31255..32415
                     /locus_tag="PLB01_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HQQ76157.1"
                     /translation="MKRTLLAAALFSLAGLRADAAEKPRLVLLPFGNLSGVERAQGAL
                     VPTLALKLTSKGWDVVRGEAVEEFLQKERIRYVDSVTPELRKKLLSSMGAEALVSGSI
                     YSFAEGDNSVCSLSARMVKADGTTAWSGVTGLTSEETEGLLGLGRAGPLPQLAERSLD
                     TLLRKFPAPGGKAQAEPRGTPLLKTSPPTYKAKALAGTAVRRVCLLPFENFTPVREAP
                     RVLSDVLQHELVASGRFEVVEAAELRAALVAEKIRGLSGLENGKLAALGKRIGTSLFV
                     RGTVYRYGDPAAKNGASTPEIDLELALLDADAGRIVWMGENARKGRDYKGLFQLGALR
                     SVVALADQVLGEVVTAEGKATPKDVPAQIARRRKALEPSETTVRAGRVGGAP"
     gene            32444..34090
                     /locus_tag="PLB01_02295"
     CDS             32444..34090
                     /locus_tag="PLB01_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQQ76158.1"
                     /translation="MRLYLRYLLTFLLPLALLAFAVAALGAPTPKPVAVVNGEAITPT
                     DLKAEFKARHGGHEKFLLGEAEAKKFLEVVIDSKLLVQEAYRLELDQQKDIVKSANEL
                     AERKAVEHLVKVEIEQKAQPKPEEVKAAWEKNTAALYRARQIAVETKAEADVVLASLL
                     AGADFEALARQCSLAPSKQHGGNLPFLGWGSMEEGWEKAVFALQPGETAGPLKTHMGW
                     EVVRLVEMQPVTRPEFEKAKSKIEGTLKKRNLEERKKALSDFLWKKYGAKRTAADLSF
                     EGLAAAAKAKSEEPVATWNGGGKATVAEILPHLDPAVLGPLPKERAAELIDAEVRQAV
                     DAPLAALEAKERKYEELPEIADEVKRHRETLMLGALYSDYILKGVKVTDEDVKAYYEA
                     HRKDLVTPEKRRVAHIVVATEDEAKDVRKRLDAGESFSSLVKAKSTDTATVKQMGDLG
                     FITANQVPPDFKGILALKEGDVSDPLKTKFGWHVVKVTKIEASRPLTLAESRDDIRKK
                     LTDDRNREKRASWVKQIRAASKIKVNESGIQAFLKDAGID"
     gene            34101..>34606
                     /locus_tag="PLB01_02300"
     CDS             34101..>34606
                     /locus_tag="PLB01_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76159.1"
                     /translation="MRTAAVSAFLALALAAGCGKKPDLKVEVKSPVPAGSAAQAPAVA
                     DDHAGHNHAAAPAAAEDHTGHNHAAPASAAPASAAPAPPPRLATISGKVLETLAASDY
                     TYMRLKTKDGETWAAVTKTTVKKGQQVTVVNAAPMHGFESKTLNRKFDSLFFGSLGQP
                     GEKPSAPAA"
BASE COUNT         5493 a        12647 c        11555 g         4911 t
ORIGIN      
        1 gtacagctcg tcgatgtacg tggtgtacgc cgccgcgggg aacgcgacgt tgtcgctcgc
       61 ccacgcgtcg agcgcgtcgt acccgccttc cgtgcaggcg ctctcccaca cggacgcttc
      121 gaccgtccgc ccgggcgccg gcgcgggcgc gctcgagccc gagcgcgcgg cggcctcgat
      181 ccgggtcttc acgcggtcca tgaagacggc gagctcgggc acgtccttcg agccgtacga
      241 gagccgctgc cacgtcccgt agcgcgcggc gtccggcccc gcgaacgcgg cgaacgggat
      301 cccgccgacg ccgccggtca gcagcagctc gaacgcgtcc accgacgtct tgacgacggc
      361 gcgttccttc acgccgttgc ggagccggtc gaaggacgcc ccgacgaggc ccgggaacgc
      421 gagcggcgcg tagaacgtgc cgccgaagtc cgtccgctcg acgccgagcg gggcgagcgc
      481 gtcggccgcg tgcagcatga agtcgcggcg ctcgcgcatg tacggcgacg gccgccggag
      541 cgcggcgagc gggctctgga gcgccgcgag cgcccagtcc tgcgcgagcg tcgacggcgt
      601 cgcgacgagc cacgccgaga ggttcgcgag gatctcgcgg gccctgacgc cgttcgagcg
      661 gatctcgtcc gagaggacga gccagcacgc gcgcgcgccc gtcgaggcgc cgaacatgtc
      721 catcttcgac aggcccgaca tcgtcacgac ccagtcgcgc cactcggggt ccgcgaacgc
      781 cgggttcacg aacgagtcga cgtagtcgag gcgctggtag accgagtcgt acaggacgaa
      841 gacgcgccgc ttcacggcga cacgcacgat ctcctcgacg agcgcccggg ggtacacgcg
      901 gccggtcggg ttgtgcgggt tgttcaggat gaccgaggac accccgccct tcgcgaggat
      961 ccggtcgagc gccgcgggat cggggaggaa cgccggggcg tccgtgggga gctcgacgac
     1021 gcgcttgccc gcgcgctcga tgaaccagtc gtactcccag ctcggccgcg gcacgacgac
     1081 gcccgacccg tcgtccccga ggacctcgag gacgattgcg agccccgcgc gcgtcccggg
     1141 ggtgaagagg atctcgtccg ggagaaaccg gacgtcggac gccgggtagt ccctcaggcc
     1201 gtcgcggttg aagaagtccg cgatctcgct ccgcacgtcc atccggccga cggcgggccc
     1261 gtagctgtgg accgtgttgc gcgtgatgcg ctgggccgaa cggacgacct ccgggtgcgg
     1321 cgcgaagtcc gagttcttgt agtcgccctg cgtcaggtcg agggcgccct cctgctgaac
     1381 ccgccgcgca aaggcgcgga cgagcggctc gggagtcgaa agggcgcgcg tggagagggt
     1441 cctcacgcgc gccgttttag caccttcggg gaggcgcgac cggatctcag aaggacgctt
     1501 cgatccccgc gagaaacctc cggcccggtg ccggaaagcc gttcacttcc gcgtacgcgc
     1561 ggtcgaaggc gttcgtgacc ctcagccacg gcgaaagcga ccacacgagc gggggcagcg
     1621 tgatcgcggc gtccacgcgg aggtacgacg ggctctcgac gacggtcgtg aagtccgccg
     1681 cgtcgcggtc ggtgcgcgcg ccgacgaaga ttgccgtgac gctgaacgac cctccggcct
     1741 tgaagcccga ccccgccgtc agcgaggcgc ggttgcgggg ccggcggatc agcggggcgt
     1801 ccgcgtcgag gtccttcgcg tcgagaaccg tgtacgacgc gcgcaggaag gcccgttcgc
     1861 cgacggcggt gcggacggca acctcgacgc cgcgcgtccg ggcgtggccg atgttcgcgt
     1921 tggagtaggt ctgcgcgtcg tactggatga ggtcccggat gtcgttccag aagcccgtgg
     1981 cctccccgac gacgccgtcg aggatcgtcc actcggcgcc gatctcccag cccgtggagc
     2041 gctcgggctc caggttcggg ttccccgagt acgggtaata cagttcgccg agcgacggcg
     2101 cacggaacgc cgaacccgcg gcggcccgga gcttgagcga cggcgcggcg cgccacgcga
     2161 gcgtcacgcg cggcgagacg gcgccgccga acgcactgtg gtcgtcccag cgcacgccgg
     2221 ccgtggcgac gagcttctcg cccgcgagcg cgatgcggtc ctccacgaag agcgcgagcg
     2281 tgcgcgacgt cgcgccgtcg agcgccacgc cgaagttgct ctcgctcgag acctgcgtcc
     2341 gctcgtaatc ggcgcccgcc gcgaggcggt gagcgccaaa cgtgccggac acggcgacgc
     2401 ggccgcccgc gcggcgcgcg tccgtcgtcg aagacgtgaa gaacgggtcg gcggggtcgg
     2461 cgtactccgg ctggtcgcgc gtgtagcgca cggagaggtc caaagagaat ttctttgaaa
     2521 gtgaatacga gaagggaacc tctacggtcg tcgtgtcggc tgatgttgaa cgctggggcg
     2581 tcggggctgc gccgtcggag gggattcccg tcctgccggt ttcgcggcgg acgacgacgc
     2641 ccgccgtggc gccctcgccg agccgcgccg tcagcgcacc cgagagcgaa gtgcccgcga
     2701 aagattcgtt ctgctggtcg ccccaggaaa gagtccttcg aaagccgaga gtccccgaaa
     2761 ggacaccttc tgagaatccg aactctccga gtccctcctt tcctcccgca tttccgagcg
     2821 cgaagctcgc gtgtccgctc gcgcccgcat ccgccgcccg gcgcgtgaag atctgcacga
     2881 cgccgccgat cgcctccgag ccgtagagcg ccgagaacgg gccgcgcacg acctcgacgc
     2941 gttcgacgtt cgccgtcgag atcgccgaga ggtccgtcgc gccgaagtac gggctgttca
     3001 gcgccacgcc gtcgaggagg acgagcgtct gcgtggacga ggtcccgcgc aggaagagcg
     3061 acgtcacgcc gccgacgccg cccgactgca cgacgtccag cccgggcacg gtgcgcagga
     3121 ggtcgaggac ggtggcggct ttcgagcgcg cgatctcggc ggcgtcgatc acggtcgcgg
     3181 cggtgccgag cgagaccgac ggctccggcg cggcctcggc cgagacgacg acgtcggcgg
     3241 cgatcggcgc ggcggcgggc agaggcgcgg gttccgccgc gaggagagaa agagagcaca
     3301 ggccgggggc gagggagagc cgggagagac cgcggaaaac caaggaacac ctccgttccg
     3361 gtggcctcgc cctcccatcc ggaaggagcg gcgggcccgg cgcgcggagg cgggcgagcc
     3421 gcgacttccc gagccccgga gaaggcgacg ctgatgcgat gttcctcggg caggtctcct
     3481 ggctcccgga tcgtccgcgc ccggcccctt cccggcggcg cgaccggctg aaccgatctc
     3541 tcgccgccag tgggatctcg ccgggcgcgt ccccggtcac agtggcgggg gccgcgcggg
     3601 aatctcaccc gcttccctct ttcacccccc ggaggtcgag gggactcgag gacgatgtga
     3661 aagaacgcgg acgaacccta gcacgacccg gcgttccgtg cgagaccggt tcaagccggt
     3721 ggcgtgtcga ggacttcgcg gaccttcgtt accagagcgt tctcgctaaa cggcttgggg
     3781 aggaagccgg acgcgccgat ctcggcgagg tggggcgctg cgtccgcggc gtagcccgac
     3841 atgaagacga ccttgatcga gggacgaagc gcccgcatgc gagtcgccag ctcgcgcccg
     3901 ttcatcccgg gcatgatgag gtccgtcagg atgagatgga tgggcctcgg gtcgctctcg
     3961 acgaggtcga gcgccttcga cccgctttcc gcgacgagca cggtgtagcc gcgccgcctg
     4021 agaatcgccg ccgcgagagc ctggacggcg ggctcgtcct cgacgacgag aaccgtctcc
     4081 gctccaaggc gggagaccac ggcgggatgg actcccgaag tcgtcttctc gtcgcaccgc
     4141 ggcaggtaga ttcggaaggt cgtccctgct cccggggcgg tttccacggc gacatatccg
     4201 cccgtctgcc ggacgatgcc gtagaccgtc gcgagcccga ggcccgttcc ttttcctctt
     4261 tccttcgtcg tgtagaacgg ctcgaaaacg tgactgagcg tctcgtggtc cattccgcat
     4321 ccgtcgtccg tcacggagag gacgacgtag cgtcctgcgg ggacaaggtc ggccgcggcc
     4381 gcgaggggag cgtcgaggtc cgcgtctgcc agggcgagcg tcaatcggcc tcctccgggc
     4441 atcgcgtcgc gtgcgttcac ggcgaggttg agaaggatct gctccatctg ccccgggtcg
     4501 gctttcacgt ttccaaccac ccgcggcatc acgacgacga tctcgatgtc ctcgccgagc
     4561 agacgcttga gcatcttctc gacgtcggtc acgatcgagc tgaggtcgag gacgcgcgtc
     4621 tcgaggacct ggcggcggct gaacgccaga agctgccgcg tcagtcgcgc cgctcgttcc
     4681 gccgccttca cgatctcgcc gacgctctcg cgattgtcgg ggtcgccctc gagggtttcc
     4741 cacagaagct cgccgtatcc ctggatgacg gtgaggaggt tgttgaagtc gtgcgcgacg
     4801 ccgcccgcga gccggcccac cgcctccatc ttctgcgcct gacggatctg ggcctcgaga
     4861 tgcaggcgct cggtgaggtc ccgcgcattg acgaccacgc cgctcatccc ggtctcgtcg
     4921 ggaaggcgat ttcccacgac cgagagcgga caccatgagc cgtcgcggtg cgcgaatcgc
     4981 acctcggctt tccgaggggc cgccccggcg aaaacgagct cgtgcagccg cgacatgacc
     5041 atcgctcgat cttccgggtg gatgagctcg aagatggacc ggctcaccag ctcctccggc
     5101 ctgtatccga gagtgtccgc cacggacggg ctcgcgaaac ccaccactcc gtccggatcg
     5161 agcaccgcga tgatgtcgag gctgttctcg atgatcgacc ggtagtaggc ctcgctcttc
     5221 gcgagagctt ccgcggctcg cttgcgttcg gtgatgtcct ccttgaccgc gacgtagtgc
     5281 gtcacggttc cgccctcctc gcggaccggc gagatcgacg ctctctccca gaagagggag
     5341 ccgtcctttc tccggttgca cagctcaccc tgccagacgc ggcccgtggt cacggcctcc
     5401 cagatcgccc ggtagacttc gggaggcgtc aacccggact tcagaaagcg cgggttctga
     5461 ccgatggcct catctgccga atagcccgtc gcctcggtga actccgggtt cacgtactcg
     5521 atccgcccct cgaggtccgt gatcacgacc gtcgcgggtg tctgttcgac ggcccgccag
     5581 agcttgcgca gctcggcgtt actcaggcga agcgcctcgc gcgcgtccag cgcatcgaga
     5641 gcaaaggaga gatcccccgc cagctccgcg aggagcgcag actcctcttc gtcgatgatt
     5701 tccttttccg ccgcatagac gacgaggccc ttcgtgaccc ggcccccgcg gcggatcgga
     5761 atggccgcga gagacttgaa tccgtggcga agcatttcac ggcgccagac atcgacgagc
     5821 gggtcggttt gaacgtccga gacgatcacc gcgtgcccgt cgcgtatcgc ggtgccgagc
     5881 gggcctctgc cctcgggcgt gtcgtcccag cgcacctcga tcctccggag atatccgacc
     5941 tcgtcccctg cgctcgcgac aggctcgacg cgcatcgtct cccggtcggc gcgcccgatc
     6001 cacgcgagcg cgtaaccgcc gtgcgcgacg aggatccggc agacctcgcc gaggagagat
     6061 ccttcgtccg tcgcgcggac gaggagctgg ttcacctcgg aaatcgtccg gagcaggcgg
     6121 ttgagcttac ggatgcggcc ctccgccgcc tttcgcgccg tgatgtcgcg gacgatcgag
     6181 agatggacgg ggccgtcggg accgtccacg cgtcgcgtgc tcacctcggc cgggaaccgg
     6241 ctcccgtcgg cgcggacgtg ctcgctctcg aagacgagcc ggtgcccgaa ctccgcggcg
     6301 gcgaagcgtt caatcgcctg ttcctgctgg cccgcgggcc ggaagtccgc gacgtctcgc
     6361 cccgcgagct cctcgcggct ccagccgtac gtttcctcgg cggcacggtt cgccgcgcgc
     6421 accctccggt cctgcccgat gatgaagacg gcgtcgttcg cctgctcgaa gaggtccatg
     6481 aagcgcgcct cgctccgcgc gagcctcgcc tcgtgcgacg actcgcgcca gcgcccgagg
     6541 gcgacggccg tgccgaagaa cgcgaggagc agcgctcccc acgcgaaccc ccgctggagc
     6601 acgtccctgc ggaacggcgc gagcgctgcg ttttcgtcca ccttcacgac gaggccccag
     6661 ggcgcggcct cgagccgccg cacggcggcg aggacacgca cgccgcggta gtccacgaag
     6721 gcctggaacg attccctttc cgtcaacgcg gcccgcgcgg cgaggccagg cgtcgccagc
     6781 gggagcctga acgagagcgg ggggtccggc cggaaccgca gcggagagag gaagacaagg
     6841 tccttcccgt gcaggccgac gaggagcgac tcgctggacg ggtcgggcaa gggctggccc
     6901 agtaggtcga agagaaacgt cgacgcgtca cccaccgaga gaacggcgcc gaggatcggg
     6961 gcacggccgg agcccgcggc gtcgccggcg accggcaccg cgctcaggag acccgcgacg
     7021 ccgtccggct cgttcccgag cgcgacgcca cgagcgcccg aatggatcac gtccgccgcg
     7081 agcgccaggc ccgccgcgcc gggagcggcg ccttcgccgg acgcgcggat gcgccccgac
     7141 gggtcgagga tccagacgga tcgcacctgg tggatgcgcc ggtagtcggc gagaattcca
     7201 ccgaggtgca cggcctcggc gcccgcggct tcgtccgaca gccccggctt caggagctcg
     7261 cggacggacg ggaaggccgc gacgaacgtc agctcttccg acgcgccgag gattcgccgc
     7321 tcgatgaggt cgcgcctgag gtccgcgcgc agcgacagct cctgtttcca gccctcgatg
     7381 gccatccgcc tccggtccgg cacgaacgtc gcgaaaaacc agagagcgac ggccgcgaga
     7441 acggcgagga gaagcgacaa ccccagcgcc tgaacgtggg cccccaccaa tttagccaga
     7501 agccgccgcg aggcggtcac gcctcgacca tggattgagt ctacgctttg ggaggagcgc
     7561 cggaggcgcc ggccttcagt ccggaccggc gtcgagggcc gtcgctcgcc tgctcaaaca
     7621 gcgtccggaa gcgcgattcg ccgcgggcgt cggcgtcggc ccggaggcgc cggagacgct
     7681 ggccgattcc ccacgcgagg tcgaggacgc cgagcaatcc ggcgcaccag ccgatcgcgg
     7741 cgccccccac gcggttccag aacggctcga gcgcctcgct ctcgtccacc taaagggcct
     7801 ggccgcccgc gaggccctgg gacgccgcgt gccgccgcgg cacaacccgc gtccggcggg
     7861 ggcgcgggca tcccggcggc tgggcgcccc gaggcgtcga agacggcgac gaggccgtag
     7921 ccgtgcgccc ggcgaaactg ctccacgatc tccgcgaagc gggagtgctc ggaaccgccc
     7981 gaattcctca cgacgccctg tacggccggg aagctcgcga ggagggacgc gtcgacgagc
     8041 gagtccttca ggaaggcgtc aagcgattcc cggcggaagt cggccctcag cccgaggtcc
     8101 ttcttccagt cctcgatcag gcacggccgg aagccacgcg cggtatcggc gcggcggcgt
     8161 cacgtgcctc ggagctccaa ttcggcctcc gcgcccgggt tctcagctct tcttctgctt
     8221 gctcttcggg ttgttgagcg ccgcaaggag cgacgcgccg aaaatggaca cggacggcgc
     8281 cgcggccttc tcgcgcctcg cggtctcctc gcggtagctc cggagatcgc cgcggtcggc
     8341 ctcggccttc gcgccggacg ccgaaaggga gatgcgcttc ctctgggtgt cgaggccgat
     8401 gaccttgact tccaccttct ggccggacgc gaagtgcttc gcgaccgtcc cgggagggac
     8461 gcccgtctcg ccgttcggga tgaggcccgt gaggcctgcc tcgaggcgga cgaagacgcc
     8521 gaacacggcg acgctctcga cctcgccctt cacgaccgag ccctcggggt agcgctcgcc
     8581 gatgccggcc cacgggtcgc ccgtcgcgac ctcgcgcagc gagagcgaga ggcggcggtg
     8641 cgccgggtcg acttccttca cccagccctg cacggtctgc ccgacggcga cctgcgggtc
     8701 cttcgcggac acgcccggcg ggagcgccga gacgtgcacg aggccgtcga tgccgggctc
     8761 gacctcgacg aagaggccga agtcggcgtg gcggaccacg gcgcccgtga acgaggcgcc
     8821 gggagcgaag cgctcggcga gcgtcgccca cgggtccgcc tcgagctcct tcatcgagag
     8881 cgagatgcgt ttgccctcgc ccgcgagctt cgtgaccttg acggtgactt cctggcccac
     8941 cgtgaggacg tccttcgggt gcgcgacgcg gcggcgcgag atctccgtga cgtggacgag
     9001 cccgtccacg ccgccgaggt cgacgaacgc gccgaagtcg gtgagggagc ggacgcggcc
     9061 cgtcatgacg gagccgagct cgatcttcgc gcgggtctcc gcggccttcg cctcgttgtc
     9121 ttccttcagg agcgcgacgc gagagacgac gacccggcgg ccgtcctcgg agtactcgat
     9181 gacccggaag tcgaagttct ttccgacgaa cgccgcgagg cccgcctcgt cgtggcggcc
     9241 gaggtcgatc tgcgagaccg ggcagaacgc gcggaggccg tgcgccccgc cgatcaggac
     9301 gtcgaagccg cccttgttgc gcgcggagac cttgccggcc acggggatct tcgcctcggc
     9361 ggcggcccgg agctcggcct tcgtcttgtg cccgatcgcc agcttgcggc tgatcctcac
     9421 gtcgggcgcg gccttgatca cgacggcctc gatgcggtcg ccgaccttct cgccgtccag
     9481 ctccttgagg tccatgatcg cttcggactt cccgccgatc gaaacgagga cgacgtcggg
     9541 cgtgatgccg gcgatcgtgc cgttgacgac ctggccctcc accagcttct tgcggggccg
     9601 ggcgtcgtcg gcggccatgg cggcctcgaa ggtcttcttg tccgcggact cggctccgtc
     9661 ggcggggtga gcggcaatca gggtctgaga cacgagggaa ctcctacccg cacgcgcaag
     9721 ctccgagagg agtctccgga cggcgggagg cggattctaa ccgaagcggt aagatgagac
     9781 ctcttctccg gtgggccccg cccgaacgga gttatccctc gacccgagga gtgggaaaga
     9841 tggctgaacc cggtgtcctt tctgcgcccg tggcgccctc cgccgctgcc gctctcgcgg
     9901 ccgtggcgcc gacgcgcgac gagctcagga gatggtatgc gctcatgcac ctcgggcgca
     9961 tcctggacga caaggccccg aactacctga agcagggcct cggctggtcc taccacgcgc
    10021 cctcggcagg gcacgacggc atccaggtcg ctctcggcgt cacgttccgc cccaatgtcg
    10081 actacctgtt tccgtattac cgcgacctga cgacgtgcgt cgcggccggg ctcagcgcgg
    10141 acgagatcct cctgaacggc atgtcgaaga agcaggacgt cgcctccggc ggccgccaca
    10201 tgtcgaacca cttcgcgaag ccctcgatcc acatccagaa cgtctcgtcc tgcgtgtcga
    10261 accacgcgca gcacgcggcg ggcctcggcc gcgcgatccg gacgtacggc gcggactccg
    10321 tcgtcttctg ctcgcagggc gagtcctcga cgtccgaggg ctacttctac gaggccgtca
    10381 acggcgcctc gcgcgagaag ctgcccgtcg tcttcgtcgt gcaggacaac ggctacggga
    10441 tctccgtccc gaagagcgac cagacggcga acctctacgc ctccgacaac ttcgcgggct
    10501 ttttgaacgt caagatcgtc cactgcgacg gcaaggacct cttcgactcg ctgcgcgccc
    10561 tgcgcgaagc cgtcgcctac gtgaagtcgg gcgccggctg cgcgctcgtc cacgccgact
    10621 gcgtgcgcat caactcgcac tcgaactcgg accgccacga gctctaccgc accccggagg
    10681 agctggccgc cgcgaaggcc ctcgacccgc tcccccgcct gcgccggacg ctcgtcgagg
    10741 gcggcgcgtt caccgaggac gagatcgccg agatcgaggc ctcgaacgtc cgcctctacg
    10801 acgaggccgc ggaccgctgc cgcaaggcgc ccgacccgga ccccgcgtcc atcttcgact
    10861 tcgtctcgcc cgagccgtgg gtccccgcgg gcgacggcct cgcgaccgtg gcccgcacgc
    10921 cggcggcgga gaccgcgccc gagctcacgc tcatccaggc gctcaacggc acgctcaagg
    10981 agatgttccg cgagaacccg gacacgtaca tctgggggca ggacgtcgcc tacaaggaca
    11041 agggcggcat cttcaacgtc acgaagggca tgcagcagga gttcggggcg aagagggtct
    11101 tcaacggccc gatcgccgag gacttcatcc tcgggaccgc cgacggcttc tcgcgcttct
    11161 ccgacaagat tcgcgtcgtc gtcgagggcg ccgagttcgc ggactacttc tggcccgcgg
    11221 ccgagcagat ggtcgagatg tcgcacgagt actggcgcac gaacggccag ttcgtcccga
    11281 acgtcacggt gcgcatcgcc tcgggcggct acatcggcgg cggcctctac cactcgcaga
    11341 acatcgaagg ctggctcacg acgctccccg gcatccgcgt cgtcgtcccc gcgttcgcgg
    11401 acgacgcggc gggcctgctg cgcacggcga tccgctcccg cggcatcacg gtgtacctcg
    11461 agcccaagta cctctacaac gcgcgcgagg cgcggacgca cgtgcccgcg gacttcgccg
    11521 tgccgttcgg caaggcgcgc ctccgccgca ccgggaagga cctcacgatc gtcgcgttcg
    11581 ggacgcccgt ccacttcgcg ctcgacgcgg ccgccgccct cgcgaaggag gccaaggagg
    11641 tcgaggtcct cgacctgcgc tcgctcgtcc cgctcgactt cgactcgatc gccgcgagcg
    11701 tgaagaagac gtcgcgcgtc ctcgtcgtcc acgaggacaa ggtccacggc ggcttcggcg
    11761 gcgagatcgc cgcgcgcatc gcgtccgagc tcttcgagca tctcgacgcg cccgtcgacc
    11821 gcgtgggctc gacgttcacg cccgtcggct tcaaccgcat cctcgagcgc gcgatcctgc
    11881 ccgacgccac gcgcgttctc gccgcggccc ggaagacgct cgcgtactga agaacgcaag
    11941 acaagaaggg cgtgatagtt tccctcgaaa gagggattcg tcctgcgtcc tgcgcaccgc
    12001 ggcctcgcgg ccgcatcggt cctctggttc gcagcggccg ccgccgcgca gaacgtcgtc
    12061 cccgaccccg ccttcaagta cggcgtcagc gcgtggagcg cggcgggcgt cgccggcacg
    12121 ttcacgctgg cgttcgctcc ggggacgagc gtccgcccgg gttccggaag cgcgcgcctc
    12181 gaccttcagg cccccgccac cggcacgtac acggtgtgcg tcccggtcgc cgccggccgg
    12241 ccctacgtct ggggcttctc gatgtacttc cccgacccga accgcgtggc cgggctgagc
    12301 gaatacatcg cgatctacga cggcccgggc tgtaccggcg ccaacatcgg gggctactcc
    12361 ctgccgatca tcggcgtccc gggcatctgg ttcggcggca cgttcggcct cttcccaacg
    12421 ccggcgggag cgcgcagcct ccgcgccggc ttcgggggtc tcggcggcgc gatcaaccag
    12481 gcgacggcgt acgtggacga cgtgttcgtc gcgcccgccg gcaccgtccc gccgatcgag
    12541 cccgtgccgg aggacgtccc cttcctctcg acgcccggcc tcctcgcgct cggggcggcg
    12601 ctggccctgg ccgccgcgcg actcctcggg gccgccctgc cgtcctccgc gtgataggtt
    12661 tctgccgcgg ggagttccca aattgcgaat gcgtttttgt ctcgtcgccg cggccgtcgt
    12721 cgtcctcacc actccggcac gttcccagaa cctcgtcgcg gacccgtcct tccggcaggg
    12781 cctcggcgcg tggagcgacg tctacgtcat cggcgcctac acgatgacct gggtgccctc
    12841 cccgagcgtg cggcccggct cgggcagcgc gctgctctcc ttcgacgctc ccgcccgcgg
    12901 cgcgtggtcc gtctgcgttc cggtccaggg cgggcttcag tacgactacg gcttctcgat
    12961 ctactacccg gacgcggccc gcacgatcgg cctgaacgag gcgtacggcg tctacagcgg
    13021 gcccggatgc acgggcacgg acctcggcgg ctacgtgctc ccgatcgttc ccgcgcagct
    13081 gaacacgtgg atcacgcgcg ccccctatcg cttcacgact ccgccggcgg cccagagcgt
    13141 catcatcacc ttcatggcgc tgggagtcgg cggcgcgaag gcgacggcgt acgtcgacga
    13201 catctatctc gggcaggccg gcacgatccc ccccatcgat ccgccgccgg cgattccgtc
    13261 gctggcgacg cccggtctcc tcgcgctcgg agccgcgctg gccctggccg ccgcgcgcat
    13321 cctccgggcc tgacgccccc gcccgagccc gagactcgct cgcgccgccg cccgccctga
    13381 tgcggctgca tcggtgaccc gcgggactga gacccgttat cttcggggct ctcgcggcgt
    13441 cggcccgcgc gcacgacgga tccgttgctg gaggcccttt tgaagaagct cttctcgaac
    13501 ctcgcgtcgc tcaagctgac cgtcgtcctc gtgctcctga tcggcgtcgt cctgtcggcg
    13561 gggacgatcg tcgagtcgct ccacgggacg aaggctgcga agtcggtcta cgaagccacc
    13621 tggttctacg tcctcctcgc cgcgttcgcc ttcaacgtct tcgcggcgct catcgaccgc
    13681 tggccccaga cgcgctggcg catcgggttc gcgatcacgc atctctcgat gctcctgatc
    13741 ctcctcggct ccgtcgtgac cgtcgtcgcg aaggtcgagg gccagatgcc gatcgacgag
    13801 gggacgtcct cgaaccgcgt cctgcgcggc gagcggggcg agcttccgcc catcgtcctc
    13861 cccttctcgc tccacctcga cgcgttcgag ctcgacacgt accccggcac gcgcatgccc
    13921 gcgcagtacc ggagccgcct gaagatcgtc tccgggacga gcgcgcagcc caccgtcatc
    13981 gagatgaaca agccgctctc ctacggcggc ttccagttct tccagtccag ctatcacatc
    14041 gacgaggggc accgcacgac gatcctctcc gtctcgcgcg accccggcca gccgatcgtc
    14101 ttcgtgggct acacgctcct cgtcctcggg atgtgcgtgg tcttcgcgac gcggctcctc
    14161 caggcgaagc aggcggagag gcagtcgccc gcgcgcgtgc cgctcaccgt cgggattctc
    14221 gccctcgccg cgctcctctc accgtccgcg cgcgccgtcg aggtgccgga cgcggccacg
    14281 gtcgagaagc tccgcgccct ccccgtccag cacgacggcc ggacgatgcc gttcgacacg
    14341 caggcccgcg aggcgctccg gtccgtcacg ggctcttcct cgtggccagg cctcgaccac
    14401 gtcgccatgg tcgcgggctg gaccgcggac ccgaacggct gggagaacgc gcccatcgtg
    14461 agggtcggcg gaagcgccgt cgcgcaggcg gccggccttc cctccggcac gcgctacgcg
    14521 agcttcgccc agctcgtctc ctcgcaggcc tttcgggccg cggcgacggc cgcgcaccgg
    14581 gccgagcagg aggagaggaa gcccacgtcc ctcgagaagg acctcctcaa gctggagggg
    14641 cgcctctcca cgctctacgg ctacttccag gcgcagcaga tcaagccgat cccgtccccc
    14701 gacccgaagg gcgcgtggcg cccgctcccg aacaaccggt ccgccgcggc catcgcgggc
    14761 gtcgcggcgc aggtccgcgc ggccggcgcg cccgcccact accccgacga caaggcgatc
    14821 tcccgcgagc tcctctacaa ccggatgcgg ccgagccgta tcgcgtggct cttcctgctg
    14881 cccgccgcgg tcgccgcggg ctacgtcgtc gccggacgga cgaagctcag gccgctcgtc
    14941 gccgcgctcc tcctcgcggg cttcgccgcg atgacgtggg gcatcgcgct gcgctggcag
    15001 atcgcgggcc gcgtcccggc gtccaacatg tacgagtcga tgctcttcct cgggtggggc
    15061 gtcggcctct tcggcgtcgt ctcgctctta ctgcggcagc cgctcctcgt cgcgaacgcg
    15121 gccgggatgg gcgccctcgc gatgatgctc gtggaccttc tcccgatgga cccgttcatc
    15181 cacccgatga tgccggtcct gacgggcacg ccgtggctcg cgatccacgt cccgatcatc
    15241 gtcgtctcct attcggttct cgcgatggcg acgggcctcg cgcacctcgt cgtcggcgtc
    15301 gagatcttcg cgccggcgcg gcgcgacctt tcgcgccgct ggagccagct cctctactac
    15361 tacctccacg tcgggtcgat cctcctcatc gcgggtatcc tcacgggctc gatctgggcc
    15421 gcgtcgtcct ggggccgcta ctggggatgg gacccgaagg aagtctggtc gctcgtcgcg
    15481 ttcctcgcgt acatggcgat cctccacgcg cgtttcgacg agcagatccg cgacttcggc
    15541 gtcgccgtct cctccatcgc cgcgttctgg acgatcctca tgacgtacct cggcgtgaac
    15601 tacgtcctcg cggccgggct ccacgcgtac ggcttcggca gctcgaacct cgtgagcatc
    15661 atgaccgtga tcgcgctcgt cgagatcgcg ttcctcctct ggggcgcgaa ggcgaggaga
    15721 gggcgcggag gacccgccgg tctcacgctc ccggtggccg ggcgataacg gacgcgggag
    15781 gtccatggcg tcgctgatcc cgtccgtccc gctgccgtgg ttccgggtgg ccctgggcat
    15841 gaacccggcg cgcctgaggt tctggggcgt cgtcgtgccc atccacgccg tcgccttcac
    15901 ggcgctctac ctcgggaacc tcaagctcct cgacgcggcg tactccgagg cgggcgtcgc
    15961 ggccgcgcgg cgccaggtcg agcagctcgt gcgcgagctg cccacgatga tgccgagcat
    16021 cccgggcgag cggagcccgc acctcttcgg ccacctcatc tcgatgcacc agccgatccg
    16081 cctgcgcctc tacagcgtgg gagcctcgct gatctggccg ccctaccacc cggcggacgc
    16141 cgacgaggcg gcccgcgtcc agcggctcat gcacgacaac gcggtccgcg acgacgtctg
    16201 gctcgagaag agggacggcc gccagtggct gcgcggcgtc gtgcgcgtga acacgggcga
    16261 ggactgccgc ccctgtcacg ccgccgggaa gacgctcggc gccgcgtccg tcacgatcga
    16321 cttcaccgac gagctgaacg agctgcacgc gatcctgagg cggcgcgtcg gctggctgtt
    16381 cggcatctgg atcgtcctcg tcggcggcgt cgcgttcgtt gcgcagaaga cgatccgccg
    16441 gtcgctctcg cggctcgaga gcgagctcga ccacgccgcc gcgggccgcg ctccctccga
    16501 gcccgcgggg gacctgccgc tcgacgccgc ctccgcggcg ttccacggcc ggctgcgcga
    16561 cttcctccgg cgccagcgcg cccgcgaggt cgagatggtc tcgcgcctcg agcgcgtgga
    16621 ccagctcgcc accctcgggg agctatccgc gggcctcgcc cacgagatca agaacccgct
    16681 cgcggggatc cagggcgcgc tcgaggtcct caagcacgac acggcagacg atgcgacggg
    16741 ccgcctctac gacgagatgc tcggcgagct gaagcgcgtg aacacgatcc tccagcgcct
    16801 cctcgagtcg gggcggcccg cgccgctccg cctgtcgcgc acgaatctgg aacggctcat
    16861 cgccgagacc gtgacgctca tgcggcccgc gctctcccgc aagaaggtcg acctctcggc
    16921 cgcggtcgag gacgacctcc ccgagatccg cgcggacgcc gcgaaggtcc gtcaggtgct
    16981 cgtcaacctc gtccagaacg ccgccgaggc gatgggcgag tcgggcggga gcgtccgcgt
    17041 gcgcgccgga aaggtcccgg acggcgacgg cgtggtcctc gccgtggaag acgacggccc
    17101 cgggatcccg cgcgagggcc tcgaccacct cttcgagccg ttctacacga cgaagttcac
    17161 gggcacgggc ctcggcctca cgatctccaa gagcctcgtc gagcagcacg gcgggcacat
    17221 ggagttcgtc tcggagcccg gccgcggcac gacgttcttc gtcttcctcc cctccggcga
    17281 cgcggcggac gacgcctccc cgctggagaa ggcgggctga cgtggcgctc gccctgatcg
    17341 tcgaggacga gagcctcctg cgctgggcgc tgtcccggca gctcgagaga gccgggcaca
    17401 aggtccacgc ggcccccgac ctcgcggccg ccgcggagca catcacggcc caccggcccg
    17461 acgtcatcct cctcgacctc ggccttccgg acggccacgg gctggatttc ttcgaggcga
    17521 accgcgaacg gatggccgag agcaaggtca tcgtcatgac cgcgctcagc caggtcgagg
    17581 aagcggtccg tgcgatgaag ctcggcgcca tcgagttcct gacgaagccc gtggacgagg
    17641 cggacctcgt ggccctcgtg aaccgctcgc tcgcggtccg caacgaccag ctcgaggcgc
    17701 aggcgatgcg ccgctcgcgc gagctgcggc tctcgaagca ggcgatcggc gagtcggcgc
    17761 ccttcaaggg cgtgctcgac gtcatcgacc aggtcgcggc ctcggacatc ccgagcatcc
    17821 tcatccaggg cgagagcggc tccggcaaga acgtcctcgc ccgccgggtc cactcgctct
    17881 cggcccgcca ggacaagccg atcctcgagg tctcgtgcgc cacgatcccc gagaacctcc
    17941 tcgaaagcga gctgttcggc cacgagaagg gcgcgttcac ggacgccaag gggctcaagc
    18001 gcggggtctt cgagctggcc gagggcggca ccgtcgtcct cgacgagatc ggcgagctgc
    18061 gcctcgacat gcaggccaag ctcctccact tcctcgagga gcggcgcttc cgcagggtcg
    18121 gcggcacgcg cgagatccag gtcgacatcc gcgtcgtcgg cctcacgaac cgcgaccttc
    18181 aggccatggt gcgcgagaag acgtttcgca ccgacctctt ctaccgcctc aacgtcttcc
    18241 ccgtggccgt gccgccgctc cgcgagaggc ccgaggacgt cctcccgctg gcccggcact
    18301 tcctcacgaa cgcgcgctcg aacctcggca cgcgcttctc gggcttcgac gccgaggccg
    18361 agcgcctcct cgtccgctac ccgtggccgg ggaacgtccg cgagctgcgc aacgtcgtcg
    18421 agcgtgctct cgtcgtcgag aggggcgaga agatcactgc ccgttcgctc atgctcgacg
    18481 cggttcccgc ggaggaaggc gcgtccggcg ccgcggtcct cccgccgggg atcgtgcccc
    18541 tcgagacgct cgagcgcgag atgatcctcc gggcgatgct cgccacgggc ggcaacacga
    18601 cccgggcggc cgagccgctc gggatctccc gcgatcagct gcgttaccgc ctcaagaagc
    18661 tcgggatccg ggaaagcggc gactagaccg ctggaacgct ctttgcgaca cggagcgtcg
    18721 gcttgggtac tcctctgcgc attttcgtcc tcgacgaaag ccggatcacc gctctcatgg
    18781 tcgagcgcgt ggctccgcac ggcaccgtcg tcgccgcctt cacgtcgctc gcggaggcgg
    18841 cgtgcgcgtt ctcggcggcg ccgcccgacg cggccgtcgt gagcctcacg ggcccgcacg
    18901 cgccgtggcg cgaattccgc gatctctgcg cgtcccgcag gcctctggtg ccgatcctct
    18961 acgagtcctg cctctacgcg agcgccgagg aagcgggcct cgagccgaca tcccggccgg
    19021 tgctcttcct ccacacgccg gcgcctttcg ccgagttcca gcgcgccgtc gcggagctgc
    19081 tcgccgccgc gattcgtgtc aagagctcgc ccaatctcct cgcgtcggcc tgatctcaca
    19141 cagtgagaat tgttcgcgcg agcggtgggt gaaatcaccc agctgcgggg gaaatgcccc
    19201 gaaattcgaa gctgcgttca gcggcgaaag cgggcttccg gcgcgcgacg ttccctgaaa
    19261 aagactcgtc gcgctgcctc ttgctattgt gtgaggacgg cggagcgtcg gcacggaagt
    19321 attcttctca gaatgggata gcgggtcttg gcgcatcggc cgctgcggcc tcacgagagg
    19381 cctgctgctg accttgagac cggggctcag gacccgaggc gtggcgcggg gtctcgcaag
    19441 ggagcttgtt ccgtggcggc acatgctttg cgcataggag gacgaggaca ttgcatcggc
    19501 catggctgac ggtcgtcgca ctctcgggtt tctctgtgta gccggcggga tccttttctt
    19561 cgccgctttc gcctcttctt ccgctttcgc cgcgggcaag cccgccggga aggcctgcat
    19621 cgactgccac gcgcccgtcg cgaagcagct gtcgaagagg ttcgtccacg cgccgttcaa
    19681 ggacgagaag ggctgcgaga gttgccacaa gcgccacggc gtcgtgggca cgctcgcgct
    19741 gaaggacgag cagcccgcgc tctgcgtcgc ctgccacggc aaggaaggcg aggccgcgaa
    19801 ggcgaaggtc gtccacgcgc cgctcgcgaa gggctcgtgc acggcctgcc acgcgccgca
    19861 cgcgtccgac gcgaagggcc tcctcaaggc cgcgggcaac gacgcgtgct tctcctgcca
    19921 caagaaggac gccttcgcga agaagttcgt ccacgcgccc gccgcggcga gctgcctcac
    19981 gtgccacaac ccgcacggcg gcgccgagaa gcacctcctc gtcaagtccg agaaggacct
    20041 ctgcgcgtcc tgccacaagg ccggcggctc cgcgaagccg gggaagcacg cgggcctcaa
    20101 cgtcgactcc gtcgcctgca cgtcctgcca ctcaccgcac gcggccgacg agaagtcgct
    20161 catgcgcgcc gtcgtccacg cgccggcgtc ggagtgcacg tcctgccacg tcgcgggccc
    20221 cggcaagccc gccctcgcga aggccgagcc tgcgctctgc gccgactgcc actccgacaa
    20281 gaccgacgac ctcaagaagc ccgtcgtcca cgagcccttc aaggacacgt gcacgacctg
    20341 ccacgcgccg cacgcgagcg accgcaaggc gctcctcgcc tcctcgcaga aggatctctg
    20401 cggcggctgc cacgacctct cgaaggagct gacggacaag gccgtcaagt ccacgcacaa
    20461 gcccgcggcc gagggctcct gctcctcgtg ccacgagccg cacgcgggca aggcgaagca
    20521 cctcgtcaag gccgagggtg ccgcgctctg cgcgacgtgc cacgagaaga cgaaggcctg
    20581 gggcgcgaag aagaacgtcc acacgccgtt cgcgggaggc gagtgcgcgg cctgccacgt
    20641 cccgcacggc ggcccgaacc cgaagctcct cgcgaaggcc ggcggcgaga cctgcttcgc
    20701 ctgccacgag gaccagaaga aggcgttcgc gaggacgttc gcgcacgcgc ccgtccgcga
    20761 gggcgactgc gcgaagtgcc acgacccgca cggctcggac ctcgcgaagt acctcgtcaa
    20821 gccgcccgac cagctctgcg cgacctgcca cgcgcagacg atcgcgcgcg ccccgtcggc
    20881 cgacggcaag aacggcacgc cgcacccgcc gttcgcgggc ggcgagtgcg ccacctgcca
    20941 cgacccgcac gcgacgtcgc ggacgcgcct cctcaaggac gtcgcctcga agctctgcga
    21001 gggctgccac tccgacgtcg tcgacggcgt cgcgaagcag aagaaggccg gaagcggctc
    21061 cgtccaccag cccgtcgtcg cggggacgtg cgagtcctgc cacgacgcgc acggctcgaa
    21121 gcagaagggc tacctcacgg cggcgccgcg cgctttgtgc ctgaagtgtc atggtgcgct
    21181 ggaagcgcgc ctgaaaaagg ccaaggccgt cgtccacaag cccgtcgccg acggcaagtg
    21241 cctcgactgc cacggcggcc actcgtccgc ctcgcccgac ctcctcgtca agcccgtccc
    21301 ggccgtctgc gccacgtgcc acgacctgaa gtcggccgac ctcctcaaga agcacggcgg
    21361 cttctctctc gagaagacga actgctcctc ctgccacgac ccgcacgtct cccagaagaa
    21421 ggggctcttc aaccccgtct tccatcctcc gttcacggac ggggactgcg cgtcctgcca
    21481 caagcccgcg gcggccgggg ctccggcgct cgcccccgcc gcgtcgctga acgcgaaggg
    21541 ggcggcgctc tgcacgccgt gccacggaga cttccccgac cgcttcaaga aggcgtggac
    21601 gcacgcgccc gcgaaggacg gcgactgcgt cgcctgccac tcgccgcacg cgacgaagga
    21661 gaagaagctc gtcgcggcgc cgctcaagac gctctgcacg ggctgccacg acagcgtcgc
    21721 gaaggacctc gccgcgaagt ccgtccaccc gcccgtgaag gacggcctct gcgcgaagtg
    21781 ccacgacgcg cacgcgtcgg acatcaagaa gggcctcgtc aaggccggca gcgacctctg
    21841 cttcgggtgc cacgcggccg agagaaccct tgccgccaag gcgaagacgc agcacgcgcc
    21901 gttcgcgaag ggcgagtgcg ggaagtgcca cgacgcgcac gcgtccgcga accccgcgca
    21961 gctccgggag cccgtcgcga cgctctgccg gaagtgccac gacgtcgcct cgccgaaggt
    22021 ctcggcggcg cacaagggca tcccggtcgc cggcgcggac tgcgcctcct gccaccaccc
    22081 ccacgcgtcc gaccagccga agctcgtccg caacgtccag caccaggtgc tcggctcctg
    22141 cgcgcgctgc cacaacacgg ccggcgggaa gccgaaggag ctggtcgcga agacgacgga
    22201 cctctgcttc cgctgtcacg gcgccgtgaa ggtcgcctcg cagaagccga acgcgcacaa
    22261 ggcgctccag cagggctgcg tcgcgtgcca cacgccgcac gcggccgacg cgaagggcct
    22321 cccgaagggg aatgagcgcg agaactgcct ctcctgccac gcccagaaga agaagcaggc
    22381 ggagacggcg cggtccgtcc acccgctgaa ggtcgagaac ggaaggtgct cgatttgcca
    22441 cacggggcac ctctccgagt tcgggagcct gctgaagacc gctcccgaga acctctgcaa
    22501 gggctgccac aagagccacg cggagttcgc gcacccgttc gggccgggca tcgtcgaccc
    22561 gcgcacgggc cggacgatga cgtgcctctc ctgccacgac ccccatggca cgcccttcca
    22621 ggcgacgctc gtggacaacc cccagcgcgc gctctgcgtc cagtgccaca agtccgacgg
    22681 ggcttccctc ggcaccgggc acggagggac ggcgaagcgc gccccatccg gaaggcccac
    22741 accttgagcg gccggacgcg gcccgtcctc ttcgcgattc tccttgccct cgtgtcgtca
    22801 gccacgcgcg gagaagagga gaagagcgaa cagatctggc tgcgtccggt cgctcttttc
    22861 gaccagcacc tttacgaggg cgccctcgcc gagccgcgcg ggatcttcta cgacgcggcc
    22921 tcgaaggaag tctgggttgc cgacacgcgg aacggccgga tcggcgtctt cacgcccgag
    22981 ggcatcccgc tcttctcctt cggaaacacc gacaagctga gagagcccat ccgcctcgcc
    23041 gtcgcgaaga acggcgaggt gtacgtcctc ggcatcgagc gcggaaggat cgagcgcttc
    23101 agctaccggg gcgagtacct cggaccgctc tctctccccg gcgttcccgc gaagccggtc
    23161 ttcgggacta tcgcggtgga cgcggacggg aacctctggg tcggcgagaa cgagacggga
    23221 caggtcctcg gattctcgcc gtccctcaag ccgctcctgc gcctcgggtc gagcggcgac
    23281 ggagagggcc agttcctggc catcgccgga atcgccgtct cgaaggacta cgtcgtcgtc
    23341 acggaccaca cggccctcgc cgtccaggtc ttcgatcgcc gcggcaactt cgtgaagggc
    23401 tggggcaagc acgagatggg gcgcgagcac ttctcgctgc ccgagggcgt cgcgctggac
    23461 aagcagaacc ggatcatcgt cgcggacgcc ctccggcacg agatcaagct cttcgacctc
    23521 gagggcaact tcctcgaccg cttcggcggc ctcggctcga aggccggcaa cgtctcctat
    23581 ccctccgacg tcgcgatgga cgccgggggc cacgtcttcg tcgcggaaag aggcaacaac
    23641 cgaatccagg tcttcgtcga gacggccgag ccgccgctcg acgcgagatc aaaaccacag
    23701 gctgacagaa aggagcccgt ttctcccgca gaacgaacca gggtttctcc ggattcaccg
    23761 aaagattcga atcgctagct agcaagaaaa acaaatggag aaatcgatga agaagactct
    23821 cattgcggtc gccgccctcg tcgcggtgat cgccttcccc gcgatcgcgg gcgagtggca
    23881 ctcgggcgag aacaacctct gcaccgactg ccacacgatg cacttcagcc agagtcacaa
    23941 ctgggacggc acgacgcccg tctcgacgac gcctcagccg aacggcaact ggctcagcgc
    24001 cacgggcccg aacgccttcc tcctcaaggc cccggcgaac cagctctgca tgtcctgcca
    24061 cgacggccag acgttcgctc cggacgttgt cggcgcgaac ttcaacgcgt cgccgaccaa
    24121 cggccgctcc gcgggcgcca tcaacgacgt cgcgctcggc gcgccgtatg acacgtggaa
    24181 gggccacacg ctcgactcca cggcgactcc ccctgggttc gatcccacca agatcggcgc
    24241 ctccgccacc tggtatgacc cggccggcgg cctcgagtgc atcagctgcc acgcgcagca
    24301 cggcccggtc acggcctacc gcaaccttgg cccgtacgcc ctcggcggcg cggccacggc
    24361 agcccgcccg acgtacgtga tcgccacgac gaacgacacg accaaggacg tctggatcaa
    24421 catcgctgct ccgtacacgg ccggctccgg ctccgcggcg acgttcaact cctactatgc
    24481 ggacgcgaac gtctccttca accgcaacga cgcgacggtc ggaaccaaca agacgtccaa
    24541 caggatggac acgttctgtg gcgcctgcca cggcaacttc catggcggcc cggccgacac
    24601 gaacatcggc gccacggtgg cggccctcga cggcttcgtc cgtcacccga cctcgcaggt
    24661 cacgatcggc gccgcgggcg cgcagggctt cggcggccac tcggcgctct cccgttacac
    24721 gggcgcgacg acgaaggtca acgtctacgc cagcgaccgc gtcggctaca cggacgcgac
    24781 cccggggtgc gtgacgtgcc acaaggcgca cggcaaccag aacccgttcg gtctcgtgtt
    24841 cctcgaccgt aacgcgacga gcgtcggcga ggaaggcggc ttcggcgcgg acgacacgga
    24901 tacctccgtg aagaccggct accgcaacct ctgcggccag tgccacagcc agggcgacta
    24961 acttcccggc ctgagttcgg gtgggggcgg tccgatcccg cccctccccg catccgagca
    25021 gacgagggcc cacgcatgag aacacacccc gggttcctca aactgttcct ggcggcgctc
    25081 gtcatcgtcg ccgccccggc ccgagccggg gaataccact acgccgagac gctgctgtgc
    25141 tccgactgcc acgccatgca cttctcgatg cagcacgact gggacgggac gacgcccgtc
    25201 tcggcgacgg ctcagccgaa cggcgactgg ctcggcgcga ccggcccgaa ccagtacctg
    25261 ctgaaggcgc aggagagcca gctctgcctg agctgccacg acggccagac ctgggctccc
    25321 gacgtgtacg gggcgaacac cggcacggcc tacgtccgcc aggcgggcgc gatcccgacc
    25381 ggcacggcgc cctacgagca gtggaagggg cacaccctca acgtccaggc gacgcctccg
    25441 ggtgggacct cgaacatccg gctgcagtgc gtctcctgcc attcctcgca cggcaacgtc
    25501 aactaccgca acctcgacgg agcgaccccg atcatcagct acgcgagggg cacgaacaac
    25561 acgacgaagg acgtgttcct gcgctcgttc actctcggcg cgatcacgac gaactacgcc
    25621 acgcagaacg tggacttcaa cgagccgggc ccgaacgcct cggcgatggg gcgtttctgc
    25681 cgcggctgcc acaccgactt ccacgggggc cagtacgact ccaacatgtt cgacggcgtc
    25741 atcggcgtgg gcggctggat ccgccatccg accgccggcg cgaacatcgg cgccctcggc
    25801 ggcgggcatt cgagcacggc gacgctggcc gcgcatgcct accgcgttca ggtgatgagc
    25861 ccgaccggca cctgggggcc gcagggcgcc acttggggcg cccctcccgg cgatctctcc
    25921 ccctcgtgca tgagctgcca caaggcccac ggcaaccaga atccgttcgg cctcatcttt
    25981 ctcaagggca ccggcccgga actcaccgag gagggcgacg cggccggcaa cactggcccg
    26041 atgggccagc ggttgagcgc gctctgcggg cagtgccacg tccaggcttc ctgatctttc
    26101 tctgaacttc aacggagggg gccggggcgc ccggcccccc atctttcaag gttctcccga
    26161 aaggctgacg cggtgacgaa aaggctgacc accgcgcgac gtgccggcct cctcgtcacg
    26221 gtcgcggttc tcctcgcggc tgccgcctcc tggcgcgcct cgggcgggga ctggcacaac
    26281 gcggatcagc tcgcgtgctc ggactgccac acgatgcaca actcgtcgaa cggccagccc
    26341 atgcgttacg acaacgaccc gcagcccgcg ccgacgctcc tccggcactc gtcctcctac
    26401 gcgctctgca cgtactgcca cgacggctcg aacgcgaacg cgccggacgt catcggcccc
    26461 gtctcgtacg tcgcggaccc gcaggccggc ttcttcccgc aggacgtcgt gacgcgcacg
    26521 gcgaatccga acggccacga cctcgcggcc ctgaaccccg agcttccgcc gggcggcacg
    26581 acggcgatga cgctcacgtg cgtttcctgc cacgccccgc acgggaacgc gaactaccgg
    26641 aacctcgtga cggaccccgc gggcacgagc gcgaacatcg ccgtcgccgc gtaccaggcg
    26701 atcaaggcga acggctcgaa ccccgcgcag gtctacgtcc agtcgaacgt cgtccccaag
    26761 tccggcatgt cggactggtg cggcgcctgc cacggcaatt tccacggccg cacgccctcg
    26821 caggaaggca cggcgagccc gtggctccga cacccgcagg accagacgct ctcgacgagc
    26881 gcgcacgtcg actacgcgta ctggacgggg gctgtcgcga acagggtccc ggtccagaac
    26941 ccgacctcga acaccatccc gtcctcgtcc gaccaggtct tctgcctgtc ctgccacaag
    27001 gcgcacggct ctcagaacaa ggccgccctg atcttcgcgg acggcgcgac gaagctctcc
    27061 acgtgcgagc agtgccacaa caaatgaagc gcacgctcgc cgccgccgcc ttcgtcctcg
    27121 ccgcgattcc ggcggcggcg ggcgtctaca ccgccacgaa gcacgggagc acgacgacgg
    27181 gcgcccagcg cctcgggacc gagccgcgcg gcgactgcgc gcagtgccac gacgagcacg
    27241 cgagccggga cggcgccccg acgggcggcc cgttcccgta cgccctcttc gccgcggacg
    27301 acaacgcgct ctgcgcgacg tgccacgcgg gctccgccgc gaacggcatc tacacgggga
    27361 gcgcggccta cgccggctcc tcgcactcga ccggcaacac gatctggccc gggccgacgc
    27421 cgccttcccg ccccagctcg gacgccggca agtgcgtgaa ctgccacgac ccgcacggga
    27481 ggagcgacgt ctcgggcctg atcccgtcga tgaccgtcgc gcgcgaggaa tcgctctgcc
    27541 tcccctgcca cgacggctcc cccgcgacga agaacgtctc ggcgcagttc tcgaagacgt
    27601 acaggcaccc gatcgcgacc gccgggaagc acaacgaggc cgaggccggc gaccccacga
    27661 agttcgcggc gagcccgacg aacaaccgcc actccgagtg cgcggactgc cacaacccgc
    27721 acgcggctgc ctccgatcgg ttcgcgccga cggcgccgtc catctcgaac cgcctcctcg
    27781 gcgtcgggcg cgtgcgcgtg acgaacggag ccgcgggcac gaagccggtg tacacgtacc
    27841 tcggccccgg cgacccctcg cccgccggcg agtaccagat ctgcttcaag tgccactcct
    27901 cgtggacgac gcagcccggg acggccacgg acctcggcgt cctcctgaac ctcaacaacc
    27961 cgtcgttcca ccccgtcgag gcgcagggga agaaccggaa catcaacgcc aactcgttcg
    28021 tgaacggctg gacgtgggac aagctcacgt actgcacgga ctgccactcg tcggacgacg
    28081 cgacggtccg gggtccgcac gggtcgagct ggcagtacat cctgaagctc tcgtccattg
    28141 cgagcccggc ccggcgggcg accgtcatgg cctcgaccga gctgtgtttc gcgtgccatt
    28201 cgtacgcgac gtacgcgaac aacggctcgt cgaacacggt caagggctac agccggttca
    28261 acccgcccca gctgagccag gggcacacgt accacgtcgg cagccagcgc tacagctgct
    28321 acaactgcca cgcgagccac ggctcggcga cccgcccttc gctcatcgtc acgggccgga
    28381 accccgggat cacttcctac acgcagaccg cgaacggcgg aacctgcacg ccgacctgcc
    28441 acggcagcga gtcgtacacg gtgaactatg cgcggtagga ggctggtgga gctcgcgctg
    28501 gcgctggccg tcctggcgag cgcccgaaca gcgcccgccc agcaggacct cacgggccgc
    28561 gcgatcctct cgtaccagac gctcgacgcc gacgtcctca ggaccgacgg cttccaccag
    28621 acgtacgacc tgaggctcga gcgcgcggtc tcggaggcgt tccgattccg cctttcgttc
    28681 cgcggcgagg gcaacgacgg atcgacgcgc ctcgacgact cggccgcccg ggaccagaag
    28741 taccggcagt accagcccgg cgcggaggtc ctctggacgt tcgcgaacgt ccagatccag
    28801 ggcaactacg acgactaccg gacggacacg agcacgggcg acctcgccga cgagaggcac
    28861 cagcgccggg tcgtgggccg catgacgtgg acgccggaga agctgcccgc gctcacgctc
    28921 tacggcgagc agcgcgcgct cacggacgac atggcccgca tcgaccagaa ggagaacacc
    28981 gcgttcgagg cgctcgactt cacgtggaag ggcctcacgc tcggccagaa cctccggtac
    29041 cagacgctcg acgacggcgg ggcggacttc gcgaggaagt ccacggacct gcagggcctc
    29101 gcccggtacg agaacacgtt cttcggcggg aaggcggccg tctcggcgaa cgtcctcctc
    29161 ggagcctcgc gcctcgacga gcgcgcgtcc tccggccagc ccgtccgcgt cccgatccgc
    29221 gtcctcgtcg gccgggcgct ctacgcgcac gacgacacgc cgcaggacgg ccgcgacagg
    29281 ccgctccagg acctcggcgc cctgatcgac ggggacttcc tgaagcctgc cggcgtctcc
    29341 ctcgggccgg acgggacgtc gtaccagaac ctcgccgcgg acatggggcg gttcatcccg
    29401 ctggacgagt tccgcgtcta cgtgcgcgac gtctcgggcg cgcccgtgaa gacgcccggc
    29461 atggtccgtt tcgacgcgta ccggagcgac gacggcctga cgtggatccc cgtcgccggg
    29521 ggctcgcgga cgagcttcga cccggcgcgc tcggcgtacg tcgtggcctt cgcgtcgacg
    29581 aacgtgaggt acctcaaggt cgtgagcttc gggacgaacg tcctcgacac gcaggtcacg
    29641 gagatcgagg tttaccagct ccagaccttc ggcgcgggcg aggtcaagcg gacggacata
    29701 cggctcggct cgggcagcgc gaccgtgagc ggcaagccca cggagtggct gacgctcggc
    29761 tactacggcc tcttcaacac gaacacgcag acctcgacga cgcggcccga gctgacgacg
    29821 aaggactcgg accagatcgc gtcgctgcag gtggacccgg tcaagaacgt gaacctcctc
    29881 gcgcggtacg agcgccggat ctcgacgcag acggacggct tcggccagac cctggacgcg
    29941 atcacggcga cggcgcactg ggcgccgctc cggacgattg acgtcaccgc cgagtaccag
    30001 cggtcggagc aggacctcgc gggccgcacg tccacctcca acgcgtacca cggccacgtg
    30061 tacctcaagc tcctgaagtc cctcgacctc tccctcgacg ccggcgccgc gcgcgaagtc
    30121 cagtcgtcgg acaaccaccc cgcctcgcac gtgaacttca ccggcgtgag ctacgcgcag
    30181 ctgacggacg acctgaagct caccgtcaac gtctcgtact cgaagacgtc gtacgatggc
    30241 gggacgccgg aggagatcgg aaccggcctc cttctcccgg cctcgcacga cacgcggtac
    30301 tggacggagc tgtactaccg gcccggccag cagctcgcgc tgacggcccg gttcgggtac
    30361 gcctcgagcg acctctattc gggcccgacg cagggctacc ggatcgagtg gtttccgttc
    30421 gggtcgggca gcgtctcgat cgggaccatc tacgaggaag acatcgactc ggtctcgaac
    30481 cggcgcttcc ggagggtgca gctccttccg cgctgggtcc tgaacaagaa catgaccctg
    30541 gaccttaact acaccgtcat gagcagttac cttgggtccg ccgacggctc ggccgccatt
    30601 tccctgacgt cccggaactt ctgggcgacc ttcacccttg cattctgatc atggcgaaaa
    30661 aggcttgaaa aaagtgacga tgagactcgt gaaagccgcc tcggccgccc tcgcggcgct
    30721 gacccttctc gccctcgccg cgtgctcgag tggctcctct gcgaagtaca cgcacccgaa
    30781 cgccgacctc ggcgcgatca agcgcgtcgc cgtcctgccg ttcgagaacc tcacgcagga
    30841 gcggtcggca tccgacaagg tccagaaggt cttcctctcc gagctcctcg cgaccgaagc
    30901 cttcgacgtc gtcgagcccg ggctcgtcgt caaggcgcta aaggcggagc ggatcgagtc
    30961 gatcgacgcc gtggggcccg ccgaggccaa gcgcctcggg gagacgctga aggcgcaggg
    31021 gctcttcacg ggagcggtcg tggacttcgc cgaagggcgc tccgggaacg tcgccgcgcc
    31081 cgaggtcacg atccagctcc gcctcatcga ggtgcagtcg ggcgtcacga tctggacggc
    31141 gtcgaagacg aagagcggcg cgagcgcgtc ggcccgcctc ttcggcgtgg gcggccagac
    31201 gctgacggag gccgcgcgcg agctgatgcg cggccagctc aaggggctcg tgaagtgaaa
    31261 cgtacccttc tggccgcggc tttgttttcg ctggcgggcc ttcgcgcgga cgctgctgaa
    31321 aagccgcgcc tcgtccttct tcctttcgga aatctctccg gcgtcgagcg cgcccagggc
    31381 gcgctcgttc ccacgctcgc cctgaagctg acctcgaagg gctgggacgt ggtgcggggt
    31441 gaggcggtcg aagaatttct tcagaaggaa agaattcggt acgtcgactc cgtgacgccg
    31501 gagctgcgga agaagctgct gtcctcgatg ggagcggagg ccctggtctc gggatcgatc
    31561 tactccttcg cggaaggcga caacagcgtc tgctccctct ccgcgaggat ggtcaaggcg
    31621 gacggcacga cggcgtggtc gggcgtcacg ggcctgacgt ccgaagagac cgaaggcctc
    31681 ctcggcctcg gccgcgccgg gccgctcccg cagctggccg agcgcagcct cgacaccctt
    31741 ctgagaaaat tccccgcgcc gggcgggaaa gcgcaggccg agccgcgcgg cacgcccctt
    31801 ctgaaaacct cccctccgac ctacaaggcg aaagctctcg cgggcacggc cgtccgccgc
    31861 gtctgcctcc tgccgttcga gaacttcacg ccggtgcgcg aggcgccgcg cgtcctctcg
    31921 gacgtcctcc agcacgagct ggtcgcgtcc gggcgcttcg aggtcgtcga ggccgccgag
    31981 ctgcgcgccg cgctcgtcgc cgagaagatc cgcgggctct ccggtctcga gaacggcaag
    32041 ctcgcggcgc tcggaaagcg catcgggacg tcgctcttcg tgcgcggcac ggtgtaccgg
    32101 tacggagacc ccgcggcgaa gaacggcgcc tcgacgcccg agatcgacct cgagctcgcg
    32161 ctcctcgacg cggacgcggg ccgcatcgtc tggatgggcg agaacgcgcg gaagggccgc
    32221 gactacaagg gccttttcca gctcggcgcg ctccggagcg tcgtcgcgct cgccgaccag
    32281 gtgctcggcg aggtcgtcac ggccgaaggg aaggcgacgc cgaaggacgt ccctgcgcag
    32341 atcgcgcgcc gtcggaaggc gctcgagccc tcggagacga ccgttcgtgc gggtcgcgtc
    32401 ggcggggccc cctgagagaa acaaggaacg aaaggctgac gaaatgagac tctacctccg
    32461 ctacctcctc acgtttctcc tgccgctcgc gctgctggcc ttcgcggtgg ccgcgctcgg
    32521 cgcgcccacg cccaagcccg tcgccgtcgt gaacggcgag gccatcaccc ccaccgacct
    32581 caaggccgag ttcaaggcgc ggcacggcgg gcacgagaag ttcctcctcg gcgaggccga
    32641 ggcgaagaag ttcctcgagg ttgtcatcga ctcaaagctc ctcgtccagg aggcgtaccg
    32701 gctcgagctc gaccagcaga aggacatcgt gaagtccgcg aacgagctcg ccgagcggaa
    32761 ggccgtcgag cacctcgtga aggtcgagat cgagcagaag gcgcagccga agcccgagga
    32821 ggtgaaggcg gcctgggaga agaacacggc cgcgctctac cgggcgcggc agatcgccgt
    32881 ggagacgaag gccgaggccg acgtggtcct cgcgagcctc ctcgcgggcg cggacttcga
    32941 ggcgctcgcg cggcagtgct cgctcgcgcc gtcgaagcag cacggcggga acctcccctt
    33001 cctcggctgg ggctcgatgg aggagggctg ggagaaggcg gtcttcgccc tccagcccgg
    33061 cgagacggcc ggccccctga agacgcacat gggctgggag gtcgtgcggc tcgtcgagat
    33121 gcagcccgtc acgcgcccgg agttcgagaa ggcgaagtcg aagatcgagg gcacgctcaa
    33181 gaagaggaac ctcgaggaga ggaagaaggc gctctcggac ttcctctgga agaagtacgg
    33241 cgcgaagagg acggcggccg acctctcgtt cgaaggcctc gccgcggccg cgaaagcgaa
    33301 atccgaggag cccgtcgcga cctggaacgg cggcggcaag gcgaccgtcg cggagatcct
    33361 tccgcacctc gacccggccg tcctcgggcc gctcccgaag gagcgcgccg cggagctgat
    33421 cgacgcagag gtccgccagg ccgtggacgc gccgctggcc gcactcgagg cgaaggagcg
    33481 caagtacgag gagctgcccg agatcgcgga cgaggtgaag cgccacaggg agacgctcat
    33541 gctcggcgcg ctctactccg actacatcct caaaggcgtc aaggtcacgg acgaggacgt
    33601 gaaggcgtac tacgaggccc accggaagga cctcgtgacg cccgagaagc gccgcgtcgc
    33661 gcacatcgtc gtcgcgacgg aggacgaggc gaaggacgtg aggaagaggc tggacgccgg
    33721 cgagagtttc tcgagcctcg tgaaggcgaa gtccacggac accgcgacgg tgaaacagat
    33781 gggcgacctc ggcttcatca cggcgaatca ggtcccgccc gacttcaagg ggatcctcgc
    33841 gctgaaggaa ggcgacgtct ccgacccgct gaagacgaaa ttcgggtggc acgtcgtgaa
    33901 ggtcacgaag atcgaggcgt cgcgcccgct cacgctcgcg gagtcgaggg acgacatcag
    33961 gaagaagctg acggacgacc ggaaccgcga gaagcgcgcg agctgggtga agcagatccg
    34021 cgccgcctcg aagatcaagg tgaacgaatc cgggatccag gcgttcctca aggacgccgg
    34081 gatcgactga caggagaccc atgcgcaccg ccgccgtttc cgcgtttctc gccctcgcgc
    34141 tcgccgcggg ctgcgggaag aagcccgacc tcaaggtcga ggtgaaatcg cccgttccgg
    34201 ccgggtccgc cgcccaggcg cccgccgtcg cagacgatca cgccggccac aaccatgccg
    34261 ctgcgccggc cgccgccgaa gaccacacgg ggcacaacca cgccgcgccc gcttccgccg
    34321 ctcccgcttc cgccgctccc gctcccccgc cgcggctcgc gacgatctcg gggaaggtcc
    34381 tcgagacgct cgccgcgtcc gactacacgt acatgcgcct gaagacgaaa gacggcgaga
    34441 cgtgggccgc cgtgacgaag accacggtga agaaaggcca gcaggtgacc gtcgtgaacg
    34501 cggcgccgat gcacggcttc gagagcaaga cgctgaaccg gaagttcgac agcctcttct
    34561 tcggctcgct cggacagccg ggcgagaagc cctcggcgcc cgcagc
//
LOCUS       DAOMFY010000013        33096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_420_length_33096_cov_8.644018, whole genome shotgun sequence.
ACCESSION   DAOMFY010000013 DAOMFY010000000
VERSION     DAOMFY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 33096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_33096_cov_8.644018"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLB01_02305"
     CDS             <1..237
                     /locus_tag="PLB01_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HQQ76160.1"
                     /translation="PRATVLVPEAAVLTSGGVTLVAVKDEAGKARTRAVTTGAVSGGK
                     VEILSGLAGGETVLVGLTAPPADGAPVVAKENAQ"
     gene            288..3503
                     /locus_tag="PLB01_02310"
     CDS             288..3503
                     /locus_tag="PLB01_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQQ76161.1"
                     /translation="MTRRPLGASGRIAKAFLESKLTPLLVVASLLLGAFALLVTPREE
                     EPQIKVPMIDVFVALPGATAEEVERRVVSPVEKALYQIPNVDFVYSTSQPSGGMVIVR
                     FMVGTDPDQAVVRVHAKIAELAPALPPGALPPVVAPRGIDDVPVAAYTLFSKDASPMQ
                     LRQIADEVKADFTRHPRVAQVTVIGGQRRVVNVRFDRDRLASYGVSLVQAYGALSGLN
                     WRLPAGSTTSGNAEVPVEVGSLFRRAEDVGTAVIGVANGKPVWLKDVASVSDGPEEPS
                     QYAWMLTKAGDDAPAVTLAVAKKPGTNAVDLVKDLDVRLEGLRGPVVPSNVTVSKTRD
                     YGKTADEKSSELIFHVGLATFSVVLLMALFLGRREAVVVLVAVPATLALTLFASFLFG
                     YTLNRVTLFALIFAIGILVDDAIVVVENVHRHYELGWGEPRLTTVYAVDEVGNPTILA
                     TFAVVAALLPLAFVSGLMGPYMRPIPVNASAAMVFSLLVAFVVSPWLTFRLFRKFAEE
                     HLRGEEHFEAGADAKPEDRLLRFYRKLFKPLLTSAARRWTLLGGVVVLLLLSCGLFLV
                     RAAKVKMLPYDNKSELQVVLDMPEGTTLEATAAAARTLATLVKAQPEVADVQVYAGTS
                     APFNFNGLVRHYFLRSGPLVADMQVNLAHKEDRRKDSHTIAKEIRNRIAEQGRRLNAN
                     VKVTEVPPGPPVLSTMVAEIYGPDPAQRIALAREVRGIFESTPGVVDTDWLVEDASPK
                     IELSIDREKAMLSGVTPELVVKTLRIALDGMDAGLLRVDTAREAVPIVLRLDRAQRSG
                     LEGLLATTVPSQTGRLVPIRELVTVVPGKRAPFIYHKNLQPVTYVLAEMAGVAEAPVY
                     GILDMKRKVGALRGPDGKPLEIFNTALPTDSNRYALKWDGEWHVTVEVFRDMGIAFAA
                     VLVLIYVLVVGWFRSFVTPLIIMAPIPLTLIGIVPAHAAFGVFFTATSMIGFIALAGI
                     IVRNSILLVDFINLELAAGEPLEEAVVKAGAVRFRPIVLTALALVAGGGVILLDPIFQ
                     GLAVALISGVVVATALTLVVIPLLYFMYIKAAGAPPAEEEA"
     gene            3500..3781
                     /locus_tag="PLB01_02315"
     CDS             3500..3781
                     /locus_tag="PLB01_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76162.1"
                     /translation="MKMLMVLAGTECREKVEVALERAGVAGYTEVEARGFGSSGRHLG
                     SAAFPKTSTIIMSFVADEDVPKLRAALQGLEGASTRPHVVTWTLESWDA"
     gene            3792..4007
                     /locus_tag="PLB01_02320"
     CDS             3792..4007
                     /locus_tag="PLB01_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQQ76163.1"
                     /translation="MPLYEFTCEKCGATFEELVGAGLDGFGVTCPECGSEEIEKLVSR
                     FASAGASTSNGGGGGGGSCGPGSGRFT"
     gene            4066..4476
                     /locus_tag="PLB01_02325"
     CDS             4066..4476
                     /locus_tag="PLB01_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HQQ76164.1"
                     /translation="MTQPERPSVALVAETTLAFDRAVARTREALAAEGFGVLTEIDFA
                     ATMKKRLDKDVPPYLILGACHPASAFKAFSAAPEVGVLLPCNVTVSVERGKTVIRAMD
                     PASVLGLVGVPELASVGLEVRAALQRAIAHAASG"
     gene            4481..4846
                     /locus_tag="PLB01_02330"
     CDS             4481..4846
                     /locus_tag="PLB01_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ76165.1"
                     /translation="MAGPSCSAFLPATDAGCERVAVRFRALSEPTRLKIVRRLALGEA
                     CVGEIVAAVGGTQANVSKHLGVLRASDLVARTRRGPRTVYRLADPALLRMCSIVCEGV
                     ERGARERAAAVLGPATGPP"
     gene            complement(4853..5062)
                     /locus_tag="PLB01_02335"
     CDS             complement(4853..5062)
                     /locus_tag="PLB01_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76166.1"
                     /translation="MSKKSVIAVVVASLVSLGGAGALLGAPAPKAPAAGSTVPVKSVH
                     HKKKTHHPAHASKHQAAPASKTTAK"
     gene            5292..5954
                     /locus_tag="PLB01_02340"
     CDS             5292..5954
                     /locus_tag="PLB01_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76167.1"
                     /translation="MGTRVPWGIAALLGVACLGEAGYIVTHRAAASSPPGRAVTQPPQ
                     EDLDRWERRLRDEISRTGKLGDGDFDTLFGDDFFRRRFDPFAEVDRVRRKLEDGLEAS
                     ERRLFSESFRNWFSKRMDLTGITTKIADQGKDVSVTFGIPGLDEDSVRFDVNASRIRL
                     RYDARKDVKKGGGDVQTSETVEKILPLPSGADPNGFEVRKGKDEITLVFHRTKRGTQD
                     HA"
     gene            5960..6232
                     /locus_tag="PLB01_02345"
     CDS             5960..6232
                     /locus_tag="PLB01_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76168.1"
                     /translation="MSLQPEGDGETAAPELKVVFWADGVEGLDPKTKLLEKIVTLSEK
                     GEFMGAVPAMKPANGWPIGSYRVEFFIGDALSKTLSFKVVSKVGAP"
     gene            6423..8111
                     /locus_tag="PLB01_02350"
     CDS             6423..8111
                     /locus_tag="PLB01_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQQ76169.1"
                     /translation="MSLLAAAALPGRCVADPQVATAPGGSGTSEAASISAAPPPRSAG
                     APVVLDGKTLFLVTERLGPFGPQERATAAAERIQALARDRFAGDLELKLTDNETTTDV
                     FAGERVVVTVSDSDAAAAGIGRHELAAGRAQAIERAVNAARREIGLKSLVVDGLWAFL
                     ATAVLVLLFYGLKKATPAALERLESWRGTRIPAIRIQRLEFVSAARVTDGMKLVVRAL
                     RFAVSLAAVLLYIPLVLSFFPWTRGLVGSLFGYVATPLRSIWSAFLAYLPDLFFLAVI
                     VVLTRGGLKLVRLFFMGIEHGTIALKGFERDWARPTYDIVRFLIIAFAVVVAFPYIPG
                     SQSAAFKGVSLFLGVLFSLSSSSAISNIVAGAILTYTKAFRIGDRVKIADTVGDVVEK
                     SLLVTRVRTIKNVDITIPNGMVLGSHILNYSTSARDKGLILNTSVTIGYDAPWRDVHA
                     ALLAAARATDHVLETPPPFVLQTSLDDFYVSYEINVYTDEASRMAATYSDLHANIQDK
                     FNEAGIEIMSPHYSSLRDGNEVTIPPASRPKTYETPPFRFLSLQRPVTPRQSPE"
     gene            8431..9309
                     /locus_tag="PLB01_02355"
     CDS             8431..9309
                     /locus_tag="PLB01_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQQ76170.1"
                     /translation="MDEASTGTTPGGAPFNVACHLSRLGFRALLLTRVGADDAGREVV
                     AAAHACGLPRAGIQVDPRRHTARVRVRHAAGGPTFGIPARQAFDEIDALAACAAFTRF
                     RPGLLYFGTLAQRAATSRSALEALLERTTASTFLDVNLRSPWWSEETLVRSLEAATTV
                     KANEKELMILAEALRFRRAGTRGRAEALLSAFPCSRLIVTRGASGAVLFSRRGHSVRA
                     HVAPGRALPGPARDSVGAGDAFSAAFLVGLLLGWPAALTLDRANQLGAALCTIRGARP
                     ESAAFYAPFRRRWRLG"
     gene            9342..11477
                     /locus_tag="PLB01_02360"
     CDS             9342..11477
                     /locus_tag="PLB01_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQQ76171.1"
                     /translation="MLSLHGLVRGHDLELGRDADTGGQVTYVVELARALGRSPAVERV
                     DLLTRLVEDPRVSSDYARPVEELGPRARILRVPFGPRRYVRKEHLWGHLDHLVDRCLS
                     LLGESVGLPHVLHSHYADAGYVGTQLSRILGIPLVHTGHSLGRVKRQRLLDAGRREAA
                     IERQFNFRRRIAAEEETLEQASLVVASTVEEIERQWGSYENLRPRRALVIPPGIDTSR
                     FSPPAGSNAPEGAEWIDRFLREPGKPMILALCRPAPKKNLARLVEAYATDAELRERAN
                     LVLVAGNRDDLRTSEPEERRVHSELLYLIDKYDLYGKVAFPKQHRPEDVAGLYRLATG
                     RRGLFVNPALNEPFGLTLIEAAASGLPVVATRDGGPRDIVANCRNGLLFDPLDPKAIA
                     AALKDALSDGRRWRQWSRNGIKGVREHYGWPAHVERYSKAVRRVLHRERKRLRRHVTS
                     VRGATLPARLIEAGHVLVTDIDDTLLGDRESLHELLEWLRLRAPDVAFGIATGRTLPM
                     ALAILGEWGVPRPDLLVTSVGTEIHYGPSRMRDLAWTRHIRPLWRRASLADALSGLPG
                     MTPQPAMKQGEFKLSYDIDPARLWPLERLQGHLRARGLHARLIRSQDRFLDVLPVRAS
                     KGLAIRHLAYRLGLPLERFLVAGDSGNDVEMLLGDTLGVVVGNHKPELKVLEGLDRVY
                     FARASFAGGILEGIRHYGFASPVAAEVTP"
     gene            11474..13864
                     /locus_tag="PLB01_02365"
     CDS             11474..13864
                     /locus_tag="PLB01_02365"
                     /EC_number="2.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016157 - sucrose synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005985 - sucrose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose synthase"
                     /protein_id="HQQ76172.1"
                     /translation="MIEELTTFAAEHREAFLLLLERYASEGRSSLLHTDLKQGFEDVR
                     RTYEKLDLASSPLADFARAAQEAEIRDPWLFFALREALGRWRYLRVHRERLVPEEVPV
                     AEFLAFKEALVAPGSAEDGILEIDFGPFGRDFPKLTEPRSVGQGLSFLNRQLASRMFN
                     DPEEGGEKLHEFLGLHAIDGQTLLLQRRFPTTASLRTALRKAIRSVEALAEDTPWASF
                     EDVFTRMGFAPGWGDTAARTAETMGLLADLFEAPSPGALEALLARIPMISRLLVVSPH
                     GYFGQHGVLGLPDTGGQVVYILDQVRALEKEMRDRLAGQGVSVEPRILIVTRLIPDAG
                     GTTCGQRLERVAGCRSTHILRIPFRKASGEVLPHWISRFRLWPYLERFAREVEREAPA
                     ELGGRPDLVIGNYSDGGLVATLLSKRLGVTQCQVAHALEKTKYLLSALHWKELEPRYH
                     FSCQYTADLITMNAADFIITSTFQEIAGTEETVGQYESYQSFTMPGLFRVTNGIDVFD
                     PKFNIVSPGADADVYFPYSDAERRLASLAPRIEALVLGDEPVPTARGRLADKEKPIVF
                     TMARLDRVKNLTGLVEWYARSEPLRRTANLLVIGGSLDPAASVDHEERAQIERMHALF
                     NEHGLDGSVRWLGMRLERNLAGELYRWVADRRGVFVQPALFEAFGLTVVEAMASGLPT
                     FATLHGGPSEIIQNGRSGFHIDPNAGDGAAASIAEFLERCARDPGQWERISRGALQRV
                     AARYTWKSYAERVMTLSRIYGFWKFVTDLQREETKRYLQVLYRLQFLPLAAAVPQE"
     gene            13874..15094
                     /locus_tag="PLB01_02370"
     CDS             13874..15094
                     /locus_tag="PLB01_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019951974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQQ76173.1"
                     /translation="MSDFHQSGPVTTLTRLGSRPLVEIEEAVARHTRRARAALLIPCL
                     VSEMDRPALARICDEVKQSPFLDTVMISLDRADADGYKRALEYFRRLERRTVVLWNDG
                     PGVLALEEKLAASDLRLGERGKGRACWMAFGYLLAQGNIEYIALHDADVLDYDRAMLA
                     RLLYPLVDPIFNFDFCKGFYARFSDRLNGRVARLFVGPLLQSLGALLGPHPYIQFLQS
                     FRYPLSGEFAVSADLARQLRVPSDWGLEIGVLSELFRHRSPRRVCQIDIADRYDHKHQ
                     ALSADDPAAGLNRMAGDIAKHLLRTLAAADVVLSEGALKSLLAAYQRRAEDAVNSFYA
                     IAKLNGLTFVRHEEETAVATFAHALKAASEQFTVDPLGAPQIPNWARVISAVPDAADL
                     LLEAAAAEGGILGG"
     gene            complement(15133..15831)
                     /locus_tag="PLB01_02375"
     CDS             complement(15133..15831)
                     /locus_tag="PLB01_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76174.1"
                     /translation="MGKNLGSQIVLAFERLLDAFVSIVPAVMVLAVSLLAGLAVGFLL
                     RGILRALIGLRRKARKDEPSSSRQLLRAAGLRAEPERLAGEASFWTAVVVGLTVGVNA
                     LEPGAVRDVLSEGVRFLPRFLASVLLAVIGLGVGALVRRSVLLGAVNSGIPWARTGAR
                     AAYVFVVASFFAAALDHLGIGRSILVAAFSIAAGSLALTFALAFGLGGRDLAKAWLER
                     KLRAEEEDTGIRHV"
     gene            complement(15843..17114)
                     /locus_tag="PLB01_02380"
     CDS             complement(15843..17114)
                     /locus_tag="PLB01_02380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ76175.1"
                     /translation="MKELEPPLTEDVARRVRALGSVDVLVGIPSFNNERTIGHVVRAI
                     VAGLARHFPDAKAVLINSDGGSTDRTREIVERTEVGSLAEILVDAVPSSSAERIVTPY
                     HGIPGKGSAFRTIFRIAAEVDAKACCVVDSDLRSITPEWVELLLEPITRHGFEFVAPL
                     YGRHRYDGTITNTIVYPLSRALYGKRVRQPIGGDFGFSGELAARFLEFEDWEGPVAQY
                     GIDVFMTTTALAEGRRVAQSFLGAKIHDPKDPGSDLAPMMVQVVSTLFQLMERHEEAW
                     RDVGGSTPVPVFGFPYTVGLEPVPVRLDRLVEQFRQAVSDLPPIWESFLSPESLQAVR
                     LAARAPTVNLGDEAWVRIIAEAAAGFRRRTLPAGVLIGSLVPLYLGKVATFVAETEAG
                     SAEDAEARLEELALGFERGKGELVTLWGAAP"
     gene            complement(17111..17887)
                     /locus_tag="PLB01_02385"
     CDS             complement(17111..17887)
                     /locus_tag="PLB01_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQQ76176.1"
                     /translation="MTAIATDLDGTLLDADATLGRVARDALAALRRAGVPVALMTTKT
                     ESELRRWLGDTGAEFGSFENGAGIVGPGGVEIDPRALSIEALMTRLAQLGKNRGAPLI
                     PIRDLPDEALREMTGLSGDAAMRARQRQYDIPFLAPGRTPSQLRQVLAGIPDVALERG
                     GVFWHLSGRHDKGTALDRILARYRLPPPLVGLGDAEIDAPFLMRCDQVVLVPGASGVR
                     AELREVFPRAYVARHPGGEGWAEAVKRLTGGDRGETGTPS"
     gene            complement(18033..18212)
                     /locus_tag="PLB01_02390"
     CDS             complement(18033..18212)
                     /locus_tag="PLB01_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76177.1"
                     /translation="MSRAGSESVQSTSPSQSAQASTPDFATIVKEKRSPVVLIFTALA
                     ALAAAVLFFLARGGS"
     gene            complement(18273..18599)
                     /locus_tag="PLB01_02395"
     CDS             complement(18273..18599)
                     /locus_tag="PLB01_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76178.1"
                     /translation="MRNAPERLHLLVLAFALFFTCLNAPCPKYSDAADGDMSSGALAL
                     DVAAGPDGTDSDAGPDLHESTPADRTPVRTLNAGDPKPRLALDPRTPLEGRIVGFGDT
                     PPPRAA"
     gene            complement(18637..20088)
                     /locus_tag="PLB01_02400"
     CDS             complement(18637..20088)
                     /locus_tag="PLB01_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HQQ76179.1"
                     /translation="MEPEEQPPPRLADETRWYTEHAALVISTLKWAVLGAAAGLCVGF
                     GTRAFLWALKASGTWVRELTPGGVHPVWLLPLALPACVFLIRVFAPTARGHGTEAVIA
                     AIHRESGRIPWKVAPIKLLATVVTLGFGGSVGKEGPCAQIGAAIASLFADILRLNDED
                     RRRLVICGIGGGFAAVFGTPISGAVFGIEVLYLGRIEYAVLFPCLVSGIVAHLVCGVQ
                     APIPALHAGLGGLGKSELVGLSIVFGAVFGLVALLLIETMRGVERGLRRFENHPYLVA
                     AGGGVFLAAFYALVGDTYAGLGVATIENALAATGTIAVLAFLWKIVATAVTLETGGSG
                     GIVTPLFFIGATSGAALAHLLHLPSGLFAAFGFVSVVAAAANTPIAAAIMGIELLPRD
                     VGVYAALAAGTAFLLVGHRSVYASQKIGLSKSAGLDVRMDVAIGDLTRSGVQVRPGSL
                     VDRAQRAGNRIISRRPGEPPSENGSGGGSGTPP"
     gene            complement(20091..20642)
                     /gene="pal"
                     /locus_tag="PLB01_02405"
     CDS             complement(20091..20642)
                     /gene="pal"
                     /locus_tag="PLB01_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQQ76180.1"
                     /translation="MSLRLPLSLGVIAAAGFLAASCSPKPPAQVAAPAPTPTPTIVRL
                     EPTPAPTPVPPRVAEDVLPTSVDEINRRGYLKDAFFEFDKYAIEPAQRDALAADATWL
                     KAHPTVRARIEGHCDERGTAAYNMALGERRASAVREYLVGLGVDGSRLQIISYGKERP
                     FATAHDEAAWAQNRRGHFVVTAR"
     gene            20720..21208
                     /locus_tag="PLB01_02410"
     CDS             20720..21208
                     /locus_tag="PLB01_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQQ76181.1"
                     /translation="MKPTREPLEPLGKVLDFMRLIWAVDHGLQSLSKKMHASMGLTGP
                     QRVALRVLGRRPGITAGDLAGILRVHPSTLTGVLHRLEGRGLVRRTRDPEDGRRARLE
                     LTPRGRKFDVPSPGTVEAVVARVLSGIPPARIRTAAELLAAVAAALLEESAPRRGSRT
                     PK"
     gene            21354..21662
                     /locus_tag="PLB01_02415"
     CDS             21354..21662
                     /locus_tag="PLB01_02415"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ76182.1"
                     /translation="MRFRALSEPMRLKILRRLFRSEASVGEILEEVGGTQANVSKHLA
                     VLHGSALVDRRKDGTRTVYRIADPTLLRICSIVCEGVERDAREKANAILGRPKRGGRR
                     "
     gene            21782..23206
                     /locus_tag="PLB01_02420"
     CDS             21782..23206
                     /locus_tag="PLB01_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQQ76183.1"
                     /translation="MIGADAVIPGPSGDLPLRYFDFIASGRFHRDVEDELNERVLPFM
                     ANTHTEGNFTGARMTELYHAALKKIGAYVGATADDAVIPVGSGSTGAINRLIHVLGLR
                     LPDQLDERFGLSARIPPEGRPVVFRSLMEHHSNDIAWRETIGDSVYVEFDAAGRISLE
                     DLDRKLSLHANRPWKIGTFSAASNVTGILNDVHGLARVLHRHGALAFFDYAAAGPYVD
                     VDMHPAGDPEGALDAVFFSVHKFLGGPRTPGLLVAHRRLFTNRVPAEPGGGTVLYTSP
                     WDHRYLGAIESRETGGTPPIVQTIQAGLAFDLKAAVGEARTERVERDYLQRAIAAWRE
                     RDDLYILGNLGTRRLGVLSVIFRQVHHHLAARLLNDLFGIQVRAGCMCAGPYGHLLLH
                     IDEEHSKTIRHRLDEGHLGEKPGWVRISLSPTVSEGEFQALLEGVDHVARRGRELSED
                     YTQSDETGEWARRTAAAVPRRGPT"
     gene            23203..24426
                     /locus_tag="PLB01_02425"
     CDS             23203..24426
                     /locus_tag="PLB01_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HQQ76184.1"
                     /translation="MTDVRKMHPATRIIHAGQHPDPATGAVSTPIYQTSTFAFRNADE
                     GAARFQGRDPGYKYTRLGNPTVAALEECLATLEDGCGALGAATGMAAINTVYMALLEQ
                     GAHVVGTDSVYGPSRLVLENEYRRFGVASTWVDSSDPANVAAAMRPSTKMVYIETPAN
                     PTMKLTDIAACADLAHANGALLVVDNTFASPVLQQPLALGADVVVHSLTKYINGHTDV
                     VGGAIVAGSPALVDRLRKVHVAFGGTMDPHQAWLVLRGVKTLGLRMERAQANSLEVAR
                     FLEAHPGVAWVLHPGLPSHPQHALMLRQMSGPGAVFSFGVKGGLEAGKALIDSVKLAT
                     LAVSLGGVETLIEHPASMTHTGMPKREREAAGITDDLVRISVGCEDARDLIADLEQAL
                     GRAAAVALAPATSSV"
     gene            complement(24451..25365)
                     /gene="nhaR"
                     /locus_tag="PLB01_02430"
     CDS             complement(24451..25365)
                     /gene="nhaR"
                     /locus_tag="PLB01_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HQQ76185.1"
                     /translation="MYSSDVERLNYHHLQVFWTAAREGGVTRASEKLHVSQPTVTTQI
                     RALERALGQSLFTRSGRRLTLTDAGRSAYRYADEIVGLGRDLTDSMKGGSPSREVRLT
                     VGVADVLSKLIAYRIVEPALRLPEPVRLEAIEDTPERLLAELALFALDVVLADAPVGP
                     TTRVRAFSHLLGECAVSVFGAGRLARAYRRDFPRSLDGAPFLLPRQHSALRVALDDWF
                     EREGIQPRIVGEFDDSALMAAVGQTGLGLFPAPSPITREVCRQHDVRPIGAIESVRQK
                     FYAITVDRKLKHPAVIAICEAARRKLFP"
     gene            25488..25838
                     /locus_tag="PLB01_02435"
     CDS             25488..25838
                     /locus_tag="PLB01_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQQ76186.1"
                     /translation="MAINVRGYGMHVSRKIRRLVDRRVSFELSRFGGQVKAVTVNLED
                     LNGPRGGIDKRCSMQANLAPGGMVRVEKTDSELRVAIGRTAARLAHAVARALERRREA
                     PMGSLGDWREREGA"
     gene            25819..28518
                     /locus_tag="PLB01_02440"
     CDS             25819..28518
                     /locus_tag="PLB01_02440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQQ76187.1"
                     /translation="MSARAPEGPQAPWHTLSAEAVLARLATAEGGLSGEEAARRLREH
                     GPNELVASRRTSPLHLLAAQFKNVLILILLIAVGLSAFLGHTLEAVVISVILLFAALL
                     GFVQEYRAERAIEGLRRMAAPVATALRDGDEQDIAARELVPGDVVRLEAGARVPADAR
                     VLEAVNLKVEEAPLTGESQPVGKISELLAAADLAIGDRKNMVYAGTTVTYGRGSVIVV
                     ETGMRTEFGTIAGLLQGVESRCTPLQENLDRVGRVLAGAAVAVVAVILLLGILRGQPF
                     WDMVIFGIALAVAVVPEALPAVVTISLALGAQRMVRRSALVRRLPAVETLGSVSVIAS
                     DKTGTLTRGEMTARRIHVAGRTLDVSGAGYERHGAFSDAGRSIAPPPPLMMLLQAAAL
                     ASDAKLKRHGDAEGWSVKGDPTELALIVAAEKAGLHKQQLDFEFPRVAEIPFTSETKR
                     MTTVHRTLQGDVAYSKGAPEVILHGCSRLLGEDGERALGAGDRELVLGTAREMASQAL
                     RVLAVAMKSRAGLEDAEREMTFLGLVGMSDPPRPEAARAIETCERAGIKAILITGDHA
                     LTAEAVARELGMLRQGRVVTGTGLDAMSDEDLEREVGEIEVYARVSPAHKLRVVTAWQ
                     RKGHYVAMTGDGVNDAPALKKADIGIAMGITGTDVSKDAAAMTLTDDNFASIVAAVEE
                     GRGVFSNIKKYLMYLISSNVGEIGLMAGASVLGLPLPLTAVQILYVNLATDGLPALAL
                     AVDPPEADLMRRPPRDPRAGIFTRPVLTLTVLGGVWSMLVNIGLFAWALGSGRSVAEA
                     VTMTFVSLVLIQFFKAYNFRSDRNSVLERPFANKWLNLAIAWEIAALAAILYVPVLNK
                     PFGTFPLPLVDWVIVTAAAFTVSPVLEIAKWMERRGWFGSLAA"
     gene            28565..29197
                     /locus_tag="PLB01_02445"
     CDS             28565..29197
                     /locus_tag="PLB01_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HQQ76188.1"
                     /translation="MSLEAFLTSTTLVAVAEIGDKTQLLSFVLAAKLRRPYAIMGGIL
                     VATLANHALAGSVGVWLASLVAPSTLNWIVGLAFIGFGLWTLRPDTLDGDPRVLGAGA
                     FVTTTVAFFLAEMGDKTQLATVALGARFDQLAWVVLGTTLGMMIANVPAVIVGEALAH
                     RIPMKTVRWIAATLFVAAGIATLAGVGADRTQPRPPENPPERPPATSPVQ"
     gene            29194..30357
                     /locus_tag="PLB01_02450"
     CDS             29194..30357
                     /locus_tag="PLB01_02450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ76189.1"
                     /translation="MRSGRGQERPWFPDSRGLWDRVLHEHVSPVRIGLAVGVGVVVGC
                     SPFLGLQVAIAAVLAIAFRLNKLAVFMGLQVSAPPLLPFVLFAGAQLGELVRHGQLLP
                     MDLDALRAMPTREIVHILFLDLLAGGTLLGLVLGTSLGTVAARVAMRRASPDSHLSRG
                     EMAQMGARLPALSFPLRQYVRWKILFDPVYALVASRLKGVREILDLGAGLGIMAAVLA
                     HRDPERRVVIVEWDVRKVAGARALLSGLSKIEIVCADARLFPTAPVESILLLDVLHYV
                     EPFAQDAWLGRCASGLKPGGLLIIREHDASHKGAVAEGFERLAVKLGWNRGPGFHPRR
                     TIDLVHRLESLGLAVETIKDGTADGLTLFVARNSYAAERETARLSSVFSRGMN"
     gene            complement(30451..30990)
                     /locus_tag="PLB01_02455"
     CDS             complement(30451..30990)
                     /locus_tag="PLB01_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76190.1"
                     /translation="MKRNLTALALLAALAALPVLAQQGSPAGRGPGSGTAAAARIDVT
                     KPSTVIGDVVSLKGGPGLGMPTLAVKVGGKEEAYVLGSYRYLTAQKFAPAAGDAVRLT
                     LYTCVECPQGFVVAEVENLTQKTVLKLRNADGTPVFAGRMGGAGHGRGAGGACCDGNG
                     AQAGPMDGTGPNPACPKKS"
     gene            complement(31148..32431)
                     /locus_tag="PLB01_02460"
     CDS             complement(31148..32431)
                     /locus_tag="PLB01_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ76191.1"
                     /translation="MARILVADDDPSFRALLKDILEGEGHSVTEARDGEEALHFARRE
                     AFALVLTDRRMPARDGLALLKALRGELPSPPPVVVLTGFGTIPEAVEAVRLGAADYLT
                     KPLESPRALLAVVDRLLAPEPDAGEIVTGDARLREVLALVDRVAAANVPVLVTGESGT
                     GKELVARRLHTRSPRATGPFVAVNAASLPESLAESELFGHERGAFTGADRGRAGRFEE
                     ASGGTLFLDEIGELAPALQAKLLRVLEDRRVRRLGSEKDVAVDVRLVAATNRDLAREV
                     ETGGFRRDLYFRLAVVPVALPPLRERPGDVSLLARHFVARLAARHRLPEPELTPGALD
                     ALARHDWPGNVRELRNTLERAVVVRGGQPIREEDVKPSAASAAPALTLDEETREREAA
                     LAALRLSGGNRDDAARRLGISVRTLYYRLRRWGLN"
     gene            complement(32433..>33096)
                     /locus_tag="PLB01_02465"
     CDS             complement(32433..>33096)
                     /locus_tag="PLB01_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ76192.1"
                     /translation="ERERRERLEAERERLDAVALTGAGLAHRVRNPLAAIKGTAQLMV
                     DGPGQQVAERSRRIVAASARIEELVSQILRFARPVEPQPQSLDLAALAREAAERAGVA
                     VAVSGPTAVGAFADGGHAADVLDELLANARSHDPGGALEIAAGRAGGTAWVEVRDRGP
                     GLSVDPARAFDPYVTSRPGGTGLGLPTVRALARANGGDVTLEARAGGGCVARLTLPGE
                     RA"
BASE COUNT         4955 a        11853 c        11185 g         5103 t
ORIGIN      
        1 ccccgcgcga ccgtcctcgt ccccgaggcc gcggtcctca cgagcggcgg cgtcacgctc
       61 gtcgccgtca aggacgaggc cggcaaggcg cgcacgcgcg ccgtcacgac gggcgccgtc
      121 tccggcggca aggtcgagat cctctcgggc ctggcgggcg gcgagaccgt tctcgtcggc
      181 ctcacggccc cgccggccga cggcgcgccg gtcgtcgcga aggaaaacgc ccagtgagcg
      241 agcccactcc cggcgggccg accgcccgcg aaatcctcca tcgaaagatg acgcgccggc
      301 ccctcggcgc gtcgggccgg atcgcgaagg cgttcctcga gtcgaagctc acacccctcc
      361 tcgtcgtcgc gtcgctcctc ctcggggcgt tcgcgctcct cgtcacgccg cgcgaagagg
      421 agccccagat caaggtcccg atgatcgacg tcttcgtggc gctcccgggc gcgacggccg
      481 aagaggtcga gcggcgggtc gtctcgcccg tcgagaaggc gctctaccag atcccgaacg
      541 tcgacttcgt ctactcgacc tctcagccct cgggcggcat ggtgatcgtg cggttcatgg
      601 tcgggacgga tcccgaccag gccgtcgtcc gcgtgcacgc gaagatcgcc gagctcgcgc
      661 ccgcgctccc gccgggcgcg ctgccgcccg tcgtcgcccc gcgcgggatc gacgacgttc
      721 ccgtcgcggc gtacacgctc ttctcgaagg acgcctcgcc gatgcagctc cggcagatcg
      781 ccgacgaggt gaaggccgac ttcacgcgcc acccgcgcgt cgcgcaggtc accgtgatcg
      841 gcggacagcg gcgcgtcgtg aacgtgaggt tcgaccgcga ccgcctcgcg tcctacggcg
      901 tctcgctcgt ccaggcgtac ggcgccctgt ccggtctcaa ctggcgcctc ccggccggga
      961 gcacgacctc cggcaacgcc gaggtccccg tcgaggtcgg ctcgctcttc cgccgcgccg
     1021 aggacgtcgg cacggccgtg atcggcgtcg cgaacggcaa gccggtctgg ctcaaggacg
     1081 tcgcctccgt gtccgacggg cccgaggagc cgtcgcagta cgcgtggatg ctcacgaagg
     1141 cgggcgacga cgcgccggcc gtgaccctcg cagtcgcgaa gaagcccgga acgaatgccg
     1201 tcgacctcgt gaaggacctc gacgtccgtc tcgagggcct gaggggcccc gtggtcccgt
     1261 cgaacgtgac cgtttcgaag acgcgcgact acggcaagac ggccgacgag aagtcgtccg
     1321 agctgatctt ccacgtcggc ctcgcgacgt tctccgtcgt cctcctcatg gcgctcttcc
     1381 tcgggcggcg cgaggcggtc gtcgtcctcg tcgcagttcc cgcgacgctc gcactcacgc
     1441 tctttgcgtc gttcctcttc gggtacacgc tgaaccgcgt cacgctcttc gcgctcatct
     1501 tcgcgatcgg catcctcgtg gacgacgcga tcgtcgtcgt cgagaacgtg catcgccact
     1561 acgagctggg ctggggcgag ccgcgcctca cgaccgtcta tgcggtcgac gaggtcggca
     1621 acccgacgat cctcgcgacg ttcgccgtcg tcgcggcgct cctcccgctc gcgttcgtgt
     1681 cgggcctcat gggcccgtac atgcgcccga tccccgtcaa cgcctccgcc gcgatggtct
     1741 tctcgctcct cgtcgcgttc gtcgtctcgc cctggctcac cttccgcctc ttccggaagt
     1801 tcgccgagga gcacctccgg ggagaggagc acttcgaggc cggcgccgac gcaaagcccg
     1861 aggaccgcct cctccgcttc taccggaagc tgttcaagcc gctcctgacg agcgcggcgc
     1921 ggcgctggac gctcctcggc ggcgtcgtcg tcctgctcct cctctcctgc gggctcttcc
     1981 tcgtccgcgc ggctaaggtc aagatgctgc cgtacgacaa caagagcgag ctgcaggtgg
     2041 tcctcgacat gcccgagggc accacgctcg aagcgaccgc cgccgccgcg cgcacgctcg
     2101 cgacgctcgt gaaggcccag cccgaggtcg cggacgtgca ggtctacgcc ggcacgtcgg
     2161 ccccgttcaa cttcaacggc ctcgtgcgtc actatttcct gcgctcgggc ccgctcgtcg
     2221 cggacatgca ggtgaacctc gcgcacaaag aggaccgcag gaaggacagc cacacgatcg
     2281 ccaaggagat ccgcaaccgg atcgccgagc aaggccggcg gctgaacgcg aacgtgaagg
     2341 tcaccgaggt tccgcccggc ccgccggtcc tttcgacgat ggtcgccgag atctacgggc
     2401 cggacccggc gcagcggatc gccctggcgc gtgaggtccg cggcatcttc gaatccactc
     2461 ccggcgtcgt cgacaccgac tggctcgtcg aggacgcctc cccgaagatc gagctttcca
     2521 tcgaccggga gaaggcgatg ctctccggcg tgaccccgga gctcgtcgtg aagacgctcc
     2581 ggatcgcgct cgacggaatg gacgcgggcc tcctgcgcgt cgacaccgcg cgcgaggcgg
     2641 tgcccatcgt gctgcgcctc gaccgcgcgc agcgctccgg cctcgagggc ctcctcgcga
     2701 cgaccgtacc ctcgcagacg ggccgtctcg tcccgatccg cgagctcgtg accgtcgtcc
     2761 cgggcaagcg ggcgcccttc atctaccaca agaacctgca gcccgtgacg tacgttctcg
     2821 ccgagatggc cggcgtcgcg gaggcgcccg tctacggcat cctcgacatg aagaggaagg
     2881 tcggggcgct ccgcggcccg gacgggaagc cgctcgagat cttcaacacg gcgctcccga
     2941 ccgactcgaa ccgctacgcg ctcaagtggg acggcgagtg gcacgtcacg gtggaggtct
     3001 tccgggacat gggaatcgcc ttcgcggccg tcctcgtcct catctacgtc ctcgtcgtgg
     3061 ggtggttccg ttcgttcgtg acgccgctca tcatcatggc gccgatcccg ctcacgctca
     3121 tcggcatcgt gccggcgcac gccgcgttcg gcgtgttctt cacggcgacg tcgatgatcg
     3181 gattcatcgc gctcgcgggc atcatcgtga ggaactcgat ccttctcgtg gacttcatca
     3241 acctcgagct cgccgcgggc gagccgctcg aggaggccgt cgtgaaggcg ggcgccgtgc
     3301 gcttccgccc gatcgtgctg acggccctcg cgctcgtcgc gggcggaggc gtcatcctcc
     3361 tcgacccgat cttccagggc ctcgccgtcg cgctcatctc gggcgtcgtc gtcgcgaccg
     3421 ccctgaccct cgtcgtgatc ccgctcctct acttcatgta catcaaggcg gccggcgcgc
     3481 cgcccgcgga ggaagaagcg tgaagatgct catggtgctc gcgggcacgg agtgccgcga
     3541 gaaggtggaa gtggcgctcg agcgcgccgg cgtcgccggc tacacggagg tcgaggcccg
     3601 gggcttcggc tcctcgggcc gccacctcgg ctccgcggcg ttcccgaaga cgtccacgat
     3661 catcatgtcc ttcgtcgcgg acgaggacgt cccgaagctg cgtgccgctc tccagggact
     3721 ggagggagct tccacccgcc cgcacgtcgt cacctggacg ctcgagtcct gggacgcctg
     3781 aggagacgcc catgcccctc tacgagttca cctgcgagaa gtgcggcgcg acgttcgaag
     3841 agctcgtcgg cgccggcctc gacggcttcg gggtgacgtg ccccgagtgc ggcagcgagg
     3901 agatcgagaa gctcgtctcg cgcttcgcat ccgccggagc ttccacctcg aacggcggcg
     3961 gcgggggcgg cggctcgtgc gggccgggct ccggccgctt cacgtgaggg tgaccccggg
     4021 gcccgtcggg cccctccgcg ctcgacccga agcaaggaac cgcccatgac ccagcccgaa
     4081 agaccgtccg tcgccctcgt ggccgaaacg acccttgcgt tcgaccgcgc cgtcgcccgc
     4141 acgcgcgagg ctctcgcggc cgagggattc ggcgtcctca ccgagatcga cttcgccgcc
     4201 acgatgaaga agaggctcga caaggacgtt ccgccgtacc tgatcctcgg cgcgtgccat
     4261 ccggcctcgg cgttcaaggc attctcggcg gccccggagg tcggcgtcct cctgccgtgc
     4321 aacgtgaccg tgtcggtgga gcgcgggaag accgtgatcc gcgcgatgga ccccgcctcc
     4381 gtgctcggac tcgtgggcgt cccggagctc gcgtccgtcg gcctcgaggt cagggccgct
     4441 ctccagcggg cgatcgcaca tgccgccagc ggctgagttc gtggcgggcc cctcctgcag
     4501 cgcctttctc cccgcaaccg acgccggctg tgagcgggtc gcggtccgct tccgcgccct
     4561 ttcggagccg acgcggctga agatcgtccg ccggctcgcg ctcggcgaag cctgcgtggg
     4621 agagatcgtc gctgcggtcg gcgggacgca ggcgaacgtg agcaagcatc tcggcgtcct
     4681 tcgcgccagc gacctcgtgg cgcggacgag gcgggggccg cgaacggtct accggctcgc
     4741 ggaccccgcg ctcctcagga tgtgctcgat cgtgtgcgag ggcgtcgagc gcggagcacg
     4801 cgaaagggcc gccgcggtcc tcggacccgc gaccggccct ccctgacgag cgtcacttcg
     4861 ccgtcgtctt cgacgccggc gctgcctggt gcttactcgc atgcgcggga tgatgggtct
     4921 tcttcttgtg gtggacggac ttcaccggca cggtggatcc ggcggcgggc gcctttggcg
     4981 caggagcgcc caggagcgcg cccgctcctc ccagcgagac gagggatgcg acgaccacgg
     5041 cgatcacgct cttcttcgac atcttcgatc tccttcccct ggctctgggg ccggcacggg
     5101 gtgggccggc ccccggggaa gatcaatccg cgtgccaggg ggaggcgatg atattttgac
     5161 gtcaaaccat cgcacgtccc gggggcacca cgccatttct gctggtacgg ccgttgcagc
     5221 aaggttgccg tcgcgacggc cggaagcgga ccgtctggtc ccgaccatcc ggccgccgga
     5281 aggagggtcc gatgggaacc agggttccgt gggggattgc cgcgctcctt ggagtcgcgt
     5341 gtctcgggga ggcgggctac atcgtcacgc atcgcgccgc cgcttcctcg ccgcccggac
     5401 gggccgtcac gcagccgccg caggaggacc tcgaccgctg ggagcgccgc ctccgcgacg
     5461 agatctcgcg gacaggcaag ctcggcgacg gggacttcga cactctcttc ggggacgact
     5521 tcttccgccg ccgtttcgac ccctttgcgg aggttgaccg ggtccgtcgg aagctcgagg
     5581 acggtctgga agcgtccgag cgcagactct tcagcgaatc cttccggaac tggttctcga
     5641 agcggatgga cctcaccgga atcacgacga agatcgcaga ccagggcaag gacgtctccg
     5701 tgacgttcgg gatccccgga ctcgacgagg actccgtccg gttcgacgtc aatgcgagcc
     5761 gcatccgcct acgctatgac gcccgcaagg acgtgaagaa gggcggaggt gacgttcaga
     5821 cgtccgagac agtcgagaaa attcttcccc ttccgtcggg tgccgacccg aacgggttcg
     5881 aggtccgcaa gggcaaggac gagatcactc tcgtgttcca caggaccaag cgaggcacgc
     5941 aagaccatgc ctaggccact tgagcctcca gcctgagggg gacggcgaga cggccgcacc
     6001 cgagctcaag gtcgtcttct gggcggacgg cgtcgaagga ctcgacccga agacgaagct
     6061 gctcgagaag atcgtcacgc tgtccgagaa gggcgagttc atgggcgccg ttcccgcgat
     6121 gaaacccgcg aacggatggc ccatcggctc ctatcgcgtc gagttcttca tcggcgacgc
     6181 tctctccaag acgctttcgt tcaaggtcgt gagcaaagtc ggagcgccct gagtcgcgcc
     6241 ggtcgatcgt cgccagcgcg tttcggcttc gctatcggct tcggaccgcg atcgagatcc
     6301 cattccgcag cagtgacgcg cccatcaggg acgagagtct ctcgctggtg atatcttttt
     6361 ccaacatgcg cgaatgccgg aaagcgtctt ttcgatcccg gcgcctgtcc ggttttctcc
     6421 tcgtgagcct cctcgcggcc gccgcgctgc cgggccgatg cgtcgcggat ccccaggtag
     6481 cgaccgcgcc gggcggcagc ggaacatctg aagcggcatc gatctccgcc gcgcctccgc
     6541 cccgttccgc aggcgcaccg gtcgtcctcg atggaaagac gcttttcctc gtcacggaaa
     6601 ggcttggacc cttcggcccc caggagcgcg cgacggctgc tgccgagcga atccaggctc
     6661 tcgcgcgcga tcgctttgcc ggcgacctcg agttgaagct gaccgacaac gagacgacca
     6721 ccgacgtctt cgccggcgag cgtgtcgtcg ttaccgtctc cgattcggac gcggccgcgg
     6781 ccggaatcgg tcggcatgag ctggccgcgg gtcgcgccca ggcgatcgag cgtgccgtca
     6841 acgcagcccg ccgcgagatc ggactgaaga gtctcgtcgt tgacggattg tgggcgtttc
     6901 tggccacggc cgtgctcgtg cttctcttct acgggctcaa gaaagcgacg ccggcggccc
     6961 tcgagcggct cgagtcgtgg agaggaaccc ggattccggc gattcggatc cagaggctcg
     7021 agttcgtcag cgcggcccgc gtgaccgacg ggatgaagct cgtcgtgcgc gcgctgcggt
     7081 ttgccgtttc gctggcggcc gtcctcctgt acatcccgct cgttctcagc ttcttcccct
     7141 ggacgcgggg actggtgggt tcgctcttcg gttacgtcgc gacgcccctg cggtcgatct
     7201 ggagcgcctt tctggcctac ctcccggacc tcttcttcct tgccgtgatc gtcgtcttga
     7261 cgcggggggg cctcaagctc gtacgcctct tcttcatggg aatcgagcac gggaccatcg
     7321 ccctgaaggg gttcgagcgg gactgggccc gtccgaccta cgacatcgtg aggttcctga
     7381 tcattgcctt cgccgtcgtc gtggcgtttc cctacatccc gggctcgcag tccgccgcgt
     7441 tcaagggggt gtccctcttc ctcggcgtcc tgttttcgct ctcgtccagc tcggcgatct
     7501 cgaacatcgt ggccggtgcg atcctcacgt acacgaaggc gttccgcatc ggcgaccgcg
     7561 tgaagatcgc cgatacggtc ggcgacgtcg tcgagaagag cctgctcgtg acacgcgtga
     7621 ggacgatcaa gaacgtcgac atcacgatcc cgaacggcat ggtgctcggc agccacatcc
     7681 tcaactattc gacgtccgcc cgcgacaagg ggctgatcct gaacacgtcc gtcacgatcg
     7741 gctacgacgc tccgtggcgc gacgtgcacg cggcgctcct cgccgcggcc cgggcgaccg
     7801 atcacgtcct ggagactccg ccgccgttcg tcctccagac gagcctcgac gacttctacg
     7861 tctcctacga gatcaacgtc tacacggacg aggcgagccg gatggccgcg acgtactccg
     7921 acttgcacgc gaacatccag gacaagttca acgaggccgg catcgagatc atgtcccccc
     7981 actactccag cctgcgcgac ggcaacgagg tgacgattcc gccggcgtcc aggccgaaaa
     8041 cctacgagac gccgccgttc cggttcctgt ccctccagcg tccggtcaca ccgcgacagt
     8101 ccccggagta gagctcagtc cggtctgcga ggagagcggg cctcgctgaa acagagtctt
     8161 gattcactat cgtttgacgt caaacgaatt tcgcactacg atgccacttg gtcgggtcta
     8221 ccgccggaaa ggaggtcttc aagatggtgt tcaatgtccc aagaccgact ccctcaccgg
     8281 aattcaccgc ggtcttgtca cgctcgcgca atcgggtctg gccgttcttc ctcgtcgtgc
     8341 tcgccgccgc agcggctctg gtggttgcgt ttgcggcctg acaccgcgca gagcatcgga
     8401 aggcgcgtcg tcctcttcgg tgaagccctg gtggacgaag cgtcgaccgg gacgacaccg
     8461 ggcggcgcgc cgttcaacgt cgcgtgccac ctgagccgtc tcggattccg tgctctcctc
     8521 ctcacccgcg tcggcgcaga cgatgcgggc cgtgaggtcg tggccgcggc ccacgcgtgc
     8581 ggtcttccgc gagccggcat ccaggtcgac ccgcgccggc acaccgcgcg cgtccgcgtc
     8641 cggcacgccg ccggcggccc gacgttcgga attcccgcac ggcaggcgtt cgacgagatc
     8701 gacgcgcttg ccgcatgcgc ggcgttcacc cgattccggc ccggtctcct ctacttcgga
     8761 acgcttgcgc agcgcgccgc gacctcccgc tccgcgctcg aggcgctcct ggagcgaacg
     8821 acggcctcca cgtttctcga cgtgaacctc cgctcaccct ggtggagcga ggagactctc
     8881 gtgcgctcac tcgaagccgc aacgacggtc aaggcgaacg agaaggagct catgattctc
     8941 gccgaggcgc tccgattccg aagggccggg acgcgcgggc gcgccgaggc ccttctgtcc
     9001 gcgttcccgt gctcccgcct catcgtgacg cgcggcgcct cgggagcagt ccttttttcg
     9061 cggcgcgggc actccgtgag ggcccacgtc gcgccgggcc gtgcgctccc gggcccggcc
     9121 cgggactcgg tgggcgcggg cgacgcgttc tcagcggcgt tcctcgtcgg tctccttctc
     9181 ggctggccgg ccgcactgac gctcgaccgc gcgaaccagc tgggcgcggc cctctgcacg
     9241 attcggggcg cacgccccga atcggctgcc ttctatgctc ctttccgtcg ccggtggagg
     9301 ctcgggtgat cgaacgcgac gagcgcccgc tccggatcct catgctgagc ctccacgggc
     9361 tcgtgcgggg acacgacctc gagctcgggc gcgacgcgga caccgggggc caggtcacgt
     9421 acgtcgtcga gctcgcgcgc gccctcgggc ggagccccgc ggtcgagcgc gtcgacctcc
     9481 tgacccgcct cgtggaggac ccgcgcgtct cctccgacta cgcgcggccc gtcgaggaac
     9541 tcgggccccg agctcggatt ctccgggttc cgttcggccc gcgccgatac gtccggaagg
     9601 aacatctctg ggggcacctc gaccacctcg tcgaccggtg cctgtcgctg ctcggcgagt
     9661 ccgtcggcct cccccacgtc ctgcacagcc actacgcgga cgccggatat gtcgggacgc
     9721 agctgtcacg catcctcgga atcccgctcg tgcacacggg acactcgctc ggccgcgtga
     9781 agcggcagcg cctgctcgac gcgggcaggc gggaagccgc gatcgagcgg caattcaact
     9841 tccgtcgacg catcgccgcc gaggaggaga ctctcgagca ggcgtccctc gtggttgcga
     9901 gcacggtcga ggagatcgag cgccagtggg gatcgtacga gaacctgcgg ccgcggcgtg
     9961 ccctcgtgat cccgccggga atcgacacct cgcgcttctc gccgccggcc ggctcgaacg
    10021 cgccggaggg cgccgagtgg atcgaccgtt tcctccgcga gcccggcaag ccgatgattc
    10081 tcgcgctctg ccggccggcc ccgaagaaga acctcgcgcg tctcgtcgag gcgtacgcca
    10141 cggacgcgga gcttcgcgag cgcgcgaacc tcgtcctcgt cgccggaaac cgcgacgatc
    10201 tccgcacctc cgagcccgag gagcggaggg tccactcgga gctcctgtat ctgatcgaca
    10261 agtacgacct ctacgggaag gtcgcgtttc cgaagcagca tcggcccgaa gacgtggccg
    10321 gtctctaccg tcttgcgacc ggccgccggg gcttgttcgt gaaccctgcc ctgaacgagc
    10381 cgttcggcct cacgctcatc gaggcggccg cgagcggcct tccggtcgtc gcgacgcgcg
    10441 acggcggccc gcgcgacatc gtcgcgaact gtcggaacgg cctgctcttc gacccccttg
    10501 acccgaaggc gatcgccgcg gctctgaagg acgcgctgtc ggacggacgg cgctggcggc
    10561 agtggtcgcg aaacggcatc aagggcgtcc gcgagcacta cggctggccc gcgcacgtcg
    10621 agcgctactc gaaggctgtc cggcgcgttc tgcaccgcga gcgcaagcgc ctgagacggc
    10681 acgtcacctc cgtgcgtgga gcgacgctcc ccgctcgttt gatcgaggcc ggacacgtgc
    10741 tcgtgacgga catcgacgac acgctcctcg gcgaccggga gagcctgcac gagctgctcg
    10801 agtggctgcg tctgcgggcg cccgacgtgg ccttcgggat cgccaccgga agaacccttc
    10861 cgatggcgct cgcgattctc ggcgaatggg gtgtgccgcg tccggacctc ctcgtgacct
    10921 ccgtcggcac cgagattcac tacgggccgt cgcgcatgcg cgacctcgcg tggacccgcc
    10981 acatccgtcc cctctggagg agggcgagcc tggccgacgc tctctcgggt cttccgggaa
    11041 tgacacctca gcccgccatg aagcagggcg agttcaagct gagctacgac atcgacccgg
    11101 cccgcctgtg gcccctcgag cgcctccagg gccacctgcg tgcgcgcggt ctccacgcgc
    11161 gcctcatccg ctcccaggac cgctttctcg acgtcctgcc cgtgcgggcc tcgaagggtc
    11221 tcgcgatccg gcatctcgcc taccgcctcg ggctgcctct cgagcggttc ctcgtcgcgg
    11281 gcgactccgg caacgacgtc gagatgctcc tcggtgacac gctcggcgtc gtcgtcggga
    11341 accacaagcc cgagctgaag gtgctcgagg ggctcgaccg cgtctacttc gcccgggcct
    11401 cgttcgcagg cggcatcctg gaggggatcc gtcactacgg gtttgcctca cccgtcgcgg
    11461 cggaggtcac gccgtgatcg aggaactcac gacctttgcg gccgagcacc gggaggcttt
    11521 tctgctcctt ctcgagcgct acgcctcgga gggacgctcg agccttctcc acacggacct
    11581 gaagcagggt tttgaggatg tccgccggac ctacgagaag ctggacctcg cctcgtcgcc
    11641 gctcgcggat ttcgcgcggg ccgcgcagga ggccgagatc cgcgacccct ggctcttctt
    11701 cgcgctgcgc gaggccctgg ggcgctggcg ctatctccgc gtccatcggg agcggctcgt
    11761 tccggaagag gtaccggttg cggagttcct cgcgttcaag gaggcgctcg tcgccccggg
    11821 atccgccgag gacgggatcc tcgagatcga cttcgggccg ttcggccgcg acttcccgaa
    11881 actcaccgag ccgcgatccg tcggtcaggg cctctcgttc ctcaaccgcc agctcgcgag
    11941 ccggatgttc aacgatcccg aggagggcgg agagaagctc cacgaattcc tcgggctcca
    12001 cgcgatcgac ggacagaccc ttctcctcca gcggcggttc ccgacgaccg catcgctgcg
    12061 caccgcgctg cgcaaggcga tccgctcggt cgaggcgctc gcggaagaca cgccgtgggc
    12121 ctccttcgaa gacgtgttca cgcgcatggg cttcgcgccg ggctggggcg acacggccgc
    12181 ccgaacggcg gagacgatgg ggcttctcgc cgatctcttc gaggcgcctt ccccgggcgc
    12241 gctggaagcg ctcctcgccc ggatcccgat gatctcgcgc ctcctcgtcg tctcgccgca
    12301 cggctacttc ggccagcacg gcgtcctcgg ccttccggac accggcgggc aggtcgtcta
    12361 catcctcgac caggtccggg ctctcgagaa agagatgcgc gaccgcctcg ccgggcaagg
    12421 cgtctccgtg gagcctcgca tcctcatcgt gacgcgactg atcccggatg caggcgggac
    12481 gacctgcggg cagcgcctcg agcgcgtcgc ggggtgccgg tcgacgcaca tcctgcgcat
    12541 ccccttccgg aaggccagcg gagaggtgct cccgcactgg atctcgcggt tccgactgtg
    12601 gccctacctc gagcgcttcg cgcgcgaggt cgagcgcgag gctcccgcgg agctgggcgg
    12661 ccggccggac ctcgtgatcg gcaactactc cgacggaggc ctcgtcgcaa cgctgctctc
    12721 gaagaggctc ggcgtgacgc agtgccaggt cgcccacgcc ctcgagaaga cgaagtacct
    12781 cctgtcggcc ctccactgga aggagctcga gccccggtac cacttctcgt gccagtacac
    12841 cgcggatctc atcacgatga acgccgcgga cttcatcatc acgagtacgt tccaggaaat
    12901 cgccggaacc gaggagaccg tcggccagta cgagagctac cagtcgttca cgatgccggg
    12961 gctcttccgg gtcacgaacg ggatcgacgt cttcgacccg aagttcaaca tcgtgtcgcc
    13021 gggagccgat gccgacgtct acttcccgta ctcggacgcg gagcgtcggc tcgcgagcct
    13081 tgcgccgcga atcgaagcgc tcgtcctcgg cgacgagcct gtccccaccg cgcggggacg
    13141 ccttgcggac aaggagaagc ccatcgtctt cacgatggcg cgcctcgacc gcgtcaagaa
    13201 cctcacgggc ctcgtggaat ggtacgcacg ctcggagccc ctccgccgaa ccgcgaacct
    13261 cctcgtcatc ggcggcagcc tcgacccggc cgcgtcggtc gatcacgagg aacgcgcgca
    13321 gatcgaacgg atgcacgccc ttttcaacga gcacggtctc gacggatccg tccggtggct
    13381 cgggatgcgg ctcgagcgga acctcgcggg tgagctctac cgctgggtcg cggaccggcg
    13441 tggggtgttc gtgcagcccg cgctcttcga ggccttcggc ctcacggtcg tcgaggcgat
    13501 ggcgtcaggg ctcccgacgt tcgcgacgct tcacggcggc ccttcggaga tcatccagaa
    13561 cggccgctcg ggctttcaca tcgacccgaa cgccggcgac ggggcggccg cttcgatcgc
    13621 cgagttcctg gaacgctgtg cgcgggaccc cggccagtgg gagcggattt cgcgcggagc
    13681 gctccagcgc gtagccgccc gctatacgtg gaagagctac gccgagcgcg tcatgacgct
    13741 ctcgcggatc tacggattct ggaagttcgt gacggacctt cagcgcgagg agacgaaacg
    13801 atacctgcag gtcctctacc ggctgcagtt cttgcctctt gccgcggccg ttccgcagga
    13861 gtgaggtgcc gacatgagcg atttccacca gtccggtccc gtcacgacgc tcacgaggct
    13921 cgggtctcgg ccgctcgtcg aaatcgagga ggccgtggca cggcacacgc ggcgggcccg
    13981 tgccgcgctc ctgatcccgt gtctcgtctc ggagatggac cggcccgcgc tcgctcggat
    14041 ctgcgacgag gtgaagcaat cgcccttcct cgacacggtc atgatctcgc tcgaccgcgc
    14101 cgacgcggac ggatacaagc gcgccctcga gtacttccgg cgcctcgagc ggcgaaccgt
    14161 cgtcttgtgg aacgacggtc ccggcgttct cgcgctcgag gaaaagctcg cggcgagcga
    14221 cctacggctg ggcgagcgcg gcaaggggcg cgcctgctgg atggcgttcg gctacctcct
    14281 cgcgcaaggc aacatcgaat acatcgccct ccacgacgcg gacgtcctcg actacgaccg
    14341 cgcgatgctc gcgcgccttc tctacccgct cgtggacccg atcttcaact tcgacttctg
    14401 caagggcttc tacgcgcggt tctcggatcg cctgaacggg cgcgtggccc gcctcttcgt
    14461 ggggccgctg ctccagtcgc tcggcgccct cctggggccc cacccgtaca tccagttcct
    14521 ccagagcttc cgttacccgc tctccggcga attcgcggtc tctgcggacc tcgcgcggca
    14581 gctccgcgtc ccatccgact ggggcctcga gatcggcgtc ctctcggagc tcttccgcca
    14641 ccggtcgccc cggcgcgtct gccagatcga catcgccgac cgatacgacc acaagcacca
    14701 ggccctttcg gccgacgacc cggctgccgg cctgaaccgg atggcgggcg acatcgccaa
    14761 gcacctgctc cgcaccctcg ccgcggccga cgtcgttctc tcggagggag ccctgaaatc
    14821 cctcctcgcg gcctaccagc ggcgcgccga ggacgccgtg aactccttct acgcgatcgc
    14881 gaagctgaac gggctcacct tcgttcgcca cgaggaggag acggccgtcg cgacgttcgc
    14941 ccacgcgctc aaggccgcga gcgagcagtt caccgtggac ccgctcggcg cgccgcagat
    15001 cccgaactgg gcgcgcgtga tttccgccgt gcccgacgcg gccgaccttc tcctggaggc
    15061 ggccgccgcc gaagggggga tactcggggg ctgaggcggc agcgtggggg agcgggaacc
    15121 ccgggcccgc cgtcagacgt ggcggatgcc cgtgtcctcc tcctcggcac gcagctttcg
    15181 ctccagccac gccttcgcga gatcgcgccc tcccagtccg aaggcgaggg cgaacgtcag
    15241 cgcgagcgag cccgccgcaa tggagaaggc ggcgaccagg atcgaacggc cgatcccgag
    15301 gtggtcgaga gccgccgcga agaaggacgc cacgacgaac acgtacgcgg cgcgggcacc
    15361 ggtgcgtgcc cacgggattc cggagtttac ggccccgaga agcacgctgc gccgcacgag
    15421 cgcgccgact ccgagtccga tgacggcgag aagcaccgag gcgagaaaac gcggcaggaa
    15481 cctcacgccc tccgagagga cgtctcgcac ggcacccggc tcgagcgcgt tcacgcccac
    15541 ggtgagaccc acgaccacgg ccgtccagaa cgacgcttcg ccggcgaggc gttcgggctc
    15601 ggcgcgaagg ccggcggccc gaaggagctg ccgcgacgag gacggttcgt ccttccgcgc
    15661 ctttcggcgc aggccgatga gggcccgaag gatgcctcgg agcaggaagc ccacggccag
    15721 gcctgcgagg agcgacaccg cgaggaccat caccgcgggg acgatcgaga cgaacgcatc
    15781 caggaggcgt tcgaacgcga gaacgatctg actgccgaga ttctttccca tcgtcatctc
    15841 cgtcacgggg ccgcgcccca gagagtcacg agttctccct ttccacgctc gaatccgagg
    15901 gccagctcct cgagccgggc ctcggcgtcc tcggccgagc ctgcctccgt ctcggcgacg
    15961 aacgtcgcga ccttaccgag atagagcggc acgagcgagc cgatgaggac gccggccggc
    16021 agcgttcttc tccggaatcc ggccgccgcc tcggcgatga tcctcaccca cgcctcgtcc
    16081 cccaggttca cggtcggcgc acgagccgca agccgcacgg cctggaggga ctccggcgaa
    16141 aggaacgact cccagatcgg cgggaggtcg ctcacggcct ggcgaaactg ctccacgaga
    16201 cggtcgagcc ggaccggaac cggctcgagc ccgaccgtgt acgggaatcc gaagacggga
    16261 accggcgtgg agcccccgac gtctcgccag gcctcctcgt gccgctccat gagctggaag
    16321 agcgtcgaga cgacctggac catcatcggc gccaggtccg acccggggtc cttcgggtcg
    16381 tggatcttcg caccgaggaa cgactgagcg acacggcggc cctcggccag cgcggtcgtc
    16441 gtcatgaaga cgtcgatccc gtactgcgcg accggtccct cccagtcctc gaactcgaga
    16501 aagcgggccg cgagctcgcc ggagaatccg aagtcgccgc cgatcggctg gcgcacgcgt
    16561 ttgccgtaga gggcccgcga caggggatac acgatcgtgt tcgtgatcgt cccgtcgtat
    16621 cggtgtcgtc cgtagagagg agccacgaac tcgaatccat ggcgcgtgat cggctcgagc
    16681 aggagctcga cccactccgg cgtgatgctc cgaaggtcgc tgtcgacgac gcagcacgcc
    16741 ttggcgtcga cctctgcggc gatccggaag atcgtccgaa acgcgctgcc cttgccggga
    16801 atgccatggt acggcgtcac gatcctctcc gccgacgagg acgggaccgc atcgacgagg
    16861 atctcggcga ggctccccac ctcggtccgt tcgacgattt cccgcgtccg gtcggtggaa
    16921 ccgccgtcgg agttgatgag gaccgctttc gcatcgggga aatggcgcgc aagcccggcc
    16981 acgatggccc tcacgacgtg accgatcgtc cgctcgttgt tgaaggacgg gatcccgacg
    17041 aggacgtcca cggacccgag cgcacgaacc cgccgcgcga cgtcctcggt gagcgggggc
    17101 tcgagctctt tcacgatggc gttcccgttt ctccgcgatc gcctccggtc agccgcttga
    17161 cggcctctgc ccacccttcg ccgcccggat gccgcgcgac gtacgcccgt gggaaaacct
    17221 cgcggagctc cgcacgcacc cccgaggcgc caggcacgag gacgacctgg tcgcaacgca
    17281 tgaggaacgg cgcgtcgatt tctgcgtccc ccagcccgac cagcggcggc ggcaggcgat
    17341 acctcgccag gatccggtcg agcgcggttc ccttgtcgtg cctcccggac aggtgccaga
    17401 agacgccgcc ccgctcgagc gccacgtcgg ggatcccggc cagtacctgc cgcagctgcg
    17461 aaggcgtccg gccgggagcg agaaacggaa tgtcgtactg tcgctgccgc gcccgcatcg
    17521 ccgcgtcgcc cgaaaggccg gtcatctcgc gcagcgcctc gtcagggaga tctctgatcg
    17581 ggatgagcgg cgcaccacga ttctttccga gctgggcgag tcgcgtcatg agcgcctcga
    17641 tcgagagcgc ccgcgggtcg atttcgacac cccccgggcc gacgatcccg gccccgttct
    17701 caaacgagcc gaattctgca ccggtgtcgc cgagccatcg acgcagctcc gactcggtct
    17761 tggtcgtcat gagggcgact ggcacgcccg cgcgtcgaag cgcggccaga gcgtcgcggg
    17821 cgacccggcc gagcgtcgcg tcagcgtcca gcagggtccc gtcaaggtcg gtcgcgatcg
    17881 ccgtcaaggt gtccctccgg tttgttcagg tccactccac gccgtctcgc atcaatagcc
    17941 gagaatcatc ttcaacacaa gcgtgagcgc aatggcggcg ccgaggaacg tcacgacctt
    18001 cagaaggtcc tttcggtctt gaggagtgac gctcacgacc cacccctcgc gaggaagaag
    18061 agcaccgcgg cagcgagagc cgcgagcgcg gtgaagatca ggaccaccgg tgaacgcttt
    18121 tccttcacga tcgttgcgaa atcgggggtg gaggcctggg cggactggga cggactcgtg
    18181 ctctggacgg attccgaacc ggctctggac atggggactc ctttcgttcc gggattggcg
    18241 caggaggcac gcccgtgagg gcgcgaaggg agctacgccg cccggggagg aggagtgtct
    18301 ccaaacccga cgatgcgccc ctcgagcggc gtacgcggat ccagtgcgag gcgaggtttc
    18361 ggatcgccgg cgttgagcgt tcggacgggt gtgcggtcgg caggagtgct ttcgtgaaga
    18421 tccgggcccg catcggaatc ggtgccgtcc ggtcccgcgg cgacgtcgag ggcgagcgct
    18481 ccggaggaca tgtcgccgtc cgctgcgtct gaatacttcg ggcacggcgc gttcagacac
    18541 gtgaagaaga gcgcgaacgc gaggacgagc aagtggaggc gttcgggggc attcctcacg
    18601 cgaccaagcc tacaccacgc gcttcgtaac gcctcttcac ggaggcgtcc cgcttcctcc
    18661 gcctgagccg ttctcggacg gaggctcgcc cggccggcgc gaaatgatcc ggttcccggc
    18721 ccgctgggcc ctgtcgacga ggctccccgg ccggacctgg acccccgacc gcgtcaggtc
    18781 gccgatcgcg acgtccatcc gcacgtcgag ccccgcggac ttcgacagac cgatcttctg
    18841 gctcgcgtag acgctccggt gcccgacgag gagaaacgcc gttcccgccg cgagcgccgc
    18901 gtagacgccg acgtcccgcg gcagcagctc gatgcccatg atggccgctg cgatcggcgt
    18961 gttcgcggca gcggcgacga cgctgacgaa gccgaaggcg gcgaagaggc ccgagggaag
    19021 gtgcagaaga tgcgcgaggg ccgcgccgct cgtggcgccg atgaagaaaa gcggcgtcac
    19081 gatcccgccg ctcccgcccg tctcgagcgt gacggcggtc gccacgatct tccagaggaa
    19141 ggcgagcacg gcgatcgttc ccgtcgcggc gagggcgttc tcgatcgtcg cgacgccgag
    19201 ccccgcgtag gtgtcgccga cgagtgcgta aaaggccgcg aggaagacac cgcctcccgc
    19261 ggcgacgagg tacgggtggt tctcgaagcg gcgaagtccg cgttcgaccc cgcgcatcgt
    19321 ttcgatgagg agcagcgcga cgaggccgaa cacggcgccg aacacgatcg acaggccgac
    19381 gagctcggac tttccgaggc cgccgagtcc cgcgtgaagg gccggaatgg gcgcttgaac
    19441 gccgcaaacg aggtgggcga cgatgccgga cacgaggcac gggaagagca ccgcgtattc
    19501 gatccggccg aggtagagca cctcgatccc gaagacggcc cccgagatcg gcgtgccgaa
    19561 gacggccgcg aagccgccgc cgatcccgca gatcacgaga cggcgccggt cctcgtcgtt
    19621 cagccggagg atgtcggcga agaggctcgc gatcgcggcg ccgatctggg cgcaggggcc
    19681 ctccttcccg accgagccgc cgaagccgag cgtcacgacg gtcgcgagga gcttgatcgg
    19741 cgcgaccttc caggggatcc ggccggactc gcgatggatc gccgcgatga cggcttccgt
    19801 cccgtggccg cgcgccgtcg gcgcgaaaac gcggatgagg aagacacacg cgggaagggc
    19861 gagcggaagg agccaaaccg gatggacgcc gcccggcgtc agctccctca cccacgtccc
    19921 ggacgccttg agcgcccaga ggaacgcgcg cgttccgaag ccgacgcaga gacccgccgc
    19981 cgcaccgagg accgcccact tcagcgtgct gatgacgaga gcggcgtgct ccgtatacca
    20041 gcgcgtctcg tcggcgagcc tcggcggcgg ctgctcctcc ggctccacgg tcagcgtgct
    20101 gtcacgacga agtggccgcg gcggttctgg gcccaggcgg cctcgtcatg ggccgtcgcg
    20161 aacggccgct ccttgccgta ggagatgatc tggaggcgcg atccgtcgac tcccaggccc
    20221 acgagatact cccgcaccgc gctcgcgcga cgctctccga gagccatatt gtacgcggcg
    20281 gttccccgct cgtcgcagtg accctcgatc ctcgcgcgca cggtcgggtg cgccttcaac
    20341 cacgtcgcat ccgccgcgag tgcgtcccgc tgggccggct cgatggcgta cttgtcgaac
    20401 tcgaagaagg cgtctttcag atagccgcgg cggttgatct cgtccacgga cgtcggaagc
    20461 acgtcctcgg cgacacgcgg cggaactggc gtcggcgcag gcgtgggctc gagccggacg
    20521 atcgtgggcg tgggtgtcgg cgcaggcgcc gcgacctggg cgggcggctt cggggagcag
    20581 gacgccgcga ggaacccggc ggcggcgatc acgccgaggg agagaggaag acgcaatgac
    20641 atcgaaggct cctttccgga ccggagtctc gcgcactttt agtttgaggt caaatcaatg
    20701 atcgtgcgat gatctcttca tgaaacccac acgcgagccg ctggagccgc tcggcaaagt
    20761 cctcgacttc atgcgcctca tctgggccgt cgaccacggt ctccagtcgc tctcgaagaa
    20821 gatgcacgcc tcgatggggc tcacggggcc gcagcgcgtc gcgctgcgcg tcctgggccg
    20881 gcgtcccgga atcaccgcgg gcgatctcgc cggaatcctc cgggtgcacc cgagcacgct
    20941 gacgggggtc ctccaccgtc tcgagggacg cgggctcgtc cgcaggacgc gcgaccccga
    21001 ggacggccgg cgggcccggc tcgagctcac cccacgcggc cgaaagttcg acgtaccctc
    21061 ccccggcacc gtcgaggccg tcgtggcgcg agttttgtcc gggattcccc cggccaggat
    21121 ccggacggcc gcggagctcc tcgccgcggt ggcggccgcc ctgctcgaag agtcggcacc
    21181 cagacgcggt tcgcggacac cgaagtaatg cacgaccccc cgtccgcggg tccggcacgc
    21241 gcccggtctc ctccatcggc atcattgacc cgtcccgccg gggggccgat catcgccata
    21301 tggcgatgca cgctcacctg ccgctgaccg acgccggctg cgaagaggtc gcaatgcgct
    21361 tccgcgccct ctcggagccc atgcgcctca agatcctgag gcgtctcttc cggagcgagg
    21421 cgtccgtggg cgagatcctc gaggaagtcg gcggaacgca ggccaacgtc agcaagcacc
    21481 tcgccgtcct gcacggctcg gcgctcgtcg accggcgcaa ggacggcacg cgcaccgtct
    21541 accgcatcgc ggaccccacc ctcctgagaa tctgctccat cgtctgcgaa ggcgtcgagc
    21601 gcgacgcccg cgaaaaggcc aacgccatcc tcggacgccc gaagcgaggc ggccgacgct
    21661 gagctccgcc gcccacgtcc cgccccgcct cgtcgacgcc gcctcttccg agccccgcgg
    21721 ccccgtcgtc accgactctc ccgaagcgga ggctgcgttc ctcgagcgcc ttcgccgccg
    21781 cgtgatcggc gcggacgcgg tgatccccgg cccctccggc gacctccccc tccggtactt
    21841 cgacttcatc gcgtcgggcc gcttccaccg cgacgtcgag gacgagctga acgagcgcgt
    21901 cctcccgttc atggcgaaca cgcacacgga ggggaacttc accggcgccc gcatgacgga
    21961 gctgtaccac gccgccctga agaagatcgg ggcgtacgtg ggcgcgaccg cggacgacgc
    22021 cgtcatccct gtcggctcgg gctccaccgg cgcgatcaac cgcctcatcc acgtcctggg
    22081 cctccggctt cccgaccagc tcgacgagcg gttcggcctc tcggcgcgca tcccgcccga
    22141 gggccggccc gtcgtcttcc ggtcgctcat ggagcaccac tcgaacgaca tcgcctggcg
    22201 cgagacgatc ggcgactccg tttacgtgga gttcgacgcg gcggggcgca tcagcctcga
    22261 ggacctcgac cgcaagctct ccctccacgc gaaccgcccg tggaagatcg gcacgttcag
    22321 cgcggcctcg aacgtcaccg ggatcctgaa cgacgtccac ggcctcgcgc gtgtcctcca
    22381 ccggcacggc gccctcgcgt tcttcgacta cgcggccgcc gggccgtacg tggacgtcga
    22441 catgcacccg gcaggcgatc ccgagggcgc gctcgacgcg gtgttcttct ccgtacacaa
    22501 gttcctcggc gggccgcgca cgccgggcct cctcgtcgcg caccggcgcc tcttcacgaa
    22561 ccgcgtcccg gccgagccgg gcggcgggac cgtcctctac acgtcgccgt gggaccaccg
    22621 ctacctcggc gcgatcgagt cgcgcgagac cggcggcacg cctccgatcg tccagacgat
    22681 ccaggccggc ctcgcgttcg acctcaaggc ggccgtcggc gaagcgcgca cggagcgggt
    22741 cgagagggac tacctccagc gagcgatcgc ggcatggcgt gagcgcgacg acctctacat
    22801 actcggcaac ctcgggacgc ggcgcctcgg cgtgctctcc gtgatcttcc ggcaggtcca
    22861 ccaccacctc gccgcgcggc tcctgaacga ccttttcggg atccaggtcc gcgccggctg
    22921 catgtgcgcc ggcccgtacg gccacctcct cctccacatc gacgaggaac actcgaaaac
    22981 catccgccac cgcctcgacg agggacacct cggggagaaa ccggggtggg tccgcatcag
    23041 cctctcgccg acggtgagcg agggcgagtt ccaagctctc ctcgagggcg tcgaccacgt
    23101 cgcgcgccgc gggcgcgagc tctccgagga ctacacccag agcgatgaga ccggcgagtg
    23161 ggcgcggcgc acggccgccg ccgtgccaag aaggggaccg acatgaccga cgtccggaag
    23221 atgcaccctg ccacacggat catccacgcg ggccagcacc ccgatcccgc gaccggggcc
    23281 gtctcgacgc cgatctacca gacgtccacg ttcgcgttcc ggaacgccga cgagggcgcc
    23341 gcgcgctttc agggccgcga ccccggctac aagtacacgc gcctcggcaa cccgacggtc
    23401 gccgcactcg aggagtgcct cgcgacgctc gaggacggct gcggggcgct gggcgccgcg
    23461 accggcatgg ccgccatcaa cacggtctac atggccctgc tcgagcaggg ggcgcacgtc
    23521 gtcggcacgg actccgtcta cggcccgtcc cgcctcgtcc tcgagaacga gtaccgccgt
    23581 ttcggcgtcg cgtccacgtg ggtcgactcg tcggatcccg cgaacgtcgc cgccgccatg
    23641 cggccctcga cgaagatggt ctacatcgag acgccggcga acccgacgat gaagctgacc
    23701 gacatcgcgg cctgcgcgga cctcgcgcac gcgaacggcg cgctcctcgt cgtcgacaac
    23761 acgttcgcgt cgcccgtcct gcagcagccg ctcgcgctcg gggccgacgt cgtcgtccac
    23821 agcctcacga agtacatcaa cggccacacg gacgtcgtcg gcggcgcgat cgtcgcgggg
    23881 tcccccgccc tcgtcgaccg gctccgcaag gtgcacgtcg cgttcggcgg cacgatggac
    23941 ccgcaccagg cgtggctggt cctccggggc gtcaagacgc tcggcctcag gatggagcgc
    24001 gcgcaggcga actcgctcga ggtggcgcgc ttcctcgagg cccaccccgg cgtcgcgtgg
    24061 gtgctccatc cgggcctccc gagccacccg cagcacgcgc tcatgctccg gcagatgtcc
    24121 gggccgggcg ccgtcttctc gttcggcgtc aagggcgggc tcgaggcggg caaggcgctc
    24181 atcgactccg tcaagctcgc gacgctcgcc gtgagcctcg gcggcgtcga gaccctcatc
    24241 gagcacccgg cctccatgac gcacacggga atgccgaaga gagagcgcga ggccgccggc
    24301 atcaccgacg acctcgtgcg gatctccgtg ggctgcgagg acgcgcgcga cctgatcgcc
    24361 gacctcgagc aggcgctcgg gagggcggcc gccgtggcgc tcgcgcccgc gacatcttcg
    24421 gtctaggggc gacagaacct cggagcggcc tcaggggaag agcttgcgcc gggccgcctc
    24481 gcagatcgcg atcacggccg ggtgcttgag cttccggtcg accgtgatcg cgtagaactt
    24541 ctggcggacg gactcgatcg ccccgatcgg gcgcacgtca tgctggcggc acacctcgcg
    24601 cgtgatgggc gacggcgcgg gaaagaggcc gagccccgtc tgtccgaccg cggccatgag
    24661 ggcgctgtcg tcgaactcgc cgacgatgcg gggctggatt ccctcccgct cgaaccagtc
    24721 gtcaagcgcg acgcgcaggg ccgagtgctg cctcggaagc agaaagggag cgccgtccag
    24781 agagcgaggg aagtcgcgcc ggtacgcccg ggccagtcga ccggcgccga atacggaaac
    24841 tgcgcattct cccagcaggt ggctgaaggc gcggacccgc gtcgtcggcc cgacgggtgc
    24901 gtcggccagc acgacgtcga gcgcgaacag cgcgagctcg gccaggaggc gttcgggcgt
    24961 gtcctcgatc gcctcgaggc gcacgggctc gggaagcctc agggcgggtt ccacgatccg
    25021 gtaggcgatc agcttcgaca gcacgtcggc gaccccgact gtcagccgga cctcgcgtga
    25081 cggggagccg cccttcatcg agtccgtgag gtcccggccg aggccgacga tctcgtcggc
    25141 gtaacgatag gcggagcgcc ccgcgtccgt gagcgtcagc cgccggcccg aacgcgtgaa
    25201 gagactctgg cccagcgccc gttcgagcgc ccggatctgg gtcgtaacgg tcggctgcga
    25261 gacgtgcagc ttctcgctcg cccgcgtgac tccgccttcc cgggcggcag tccagaagac
    25321 ctggaggtgg tggtagttca ggcgttccac gtcgctacta tacatttaga tccttctatg
    25381 aatgctctcg aaagaatgaa tttgtacagg cgctcgctcg cgcctagctt gccgatggga
    25441 ggccgtctcc ggcgccgcag cggcgctcga ggccttggag ggacacgatg gcaatcaatg
    25501 ttcgtggcta cgggatgcac gtgagccgga agatccgcag gctcgtggat cgccgcgtgt
    25561 ccttcgagct ctcccgattc ggcgggcagg tcaaggccgt gaccgtcaac ctcgaggacc
    25621 tgaacggccc ccgcgggggc atcgacaagc ggtgctcgat gcaggcgaac ctcgccccgg
    25681 ggggcatggt gcgagtggag aagacggaca gcgagcttcg cgtcgccatc ggccgcacag
    25741 cggctcggct cgctcacgcc gtcgcgcggg cgctggagcg ccgccgcgaa gcgccgatgg
    25801 gctcgctcgg ggattggcgt gagcgcgagg gcgcctgagg gaccgcaagc cccgtggcat
    25861 acgctctcgg ccgaggccgt tctcgcacgg ctcgccacgg ccgagggggg cctgagcggc
    25921 gaggaagccg cgcgccgcct gcgcgagcac ggcccgaacg agctcgtcgc ctctcggcgg
    25981 acgtctccgc tgcacctcct cgccgcgcag ttcaagaacg tgcttatcct gatcctcctg
    26041 attgcggtcg ggctctccgc cttcctcggc cacaccctgg aggccgtcgt catctcggtg
    26101 atcctgctct tcgccgcgct gctgggcttc gtccaggagt accgggccga gcgcgccatc
    26161 gaggggctgc ggcggatggc cgcccccgtc gcgaccgcgc tgcgcgacgg cgacgagcag
    26221 gacatcgcgg cccgcgagct ggtgcccggc gacgtcgtcc ggctcgaggc cggcgccagg
    26281 gtccccgcgg acgcgcgggt cctcgaggcg gtgaatctca aggtggagga ggcgcccctg
    26341 acgggggagt cgcagccggt cggaaagatc agcgagctcc tggcggccgc cgaccttgcg
    26401 atcggcgacc ggaagaacat ggtctatgcc ggcacgaccg tcacctacgg ccgcggatcg
    26461 gtgatcgtcg tcgaaacggg aatgcggacg gagttcggga cgatcgccgg gctcctgcag
    26521 ggcgtcgaga gccggtgcac gccgctccag gagaacctcg accgcgtggg ccgcgtcctg
    26581 gcgggagccg ccgtggccgt cgtcgccgtg atcctcctcc tcggcatcct cagaggccag
    26641 ccgttctggg acatggtgat cttcgggatc gccctggccg tggcggtggt cccggaagcg
    26701 cttccggcgg tcgtgacgat ctccctggct ctcggtgcgc agcggatggt gagacgcagc
    26761 gccctggtgc gccggctgcc ggccgtggaa acgctcggga gcgtttccgt catcgcctcg
    26821 gacaagaccg gcacgctgac ccggggcgag atgaccgccc gccgaatcca cgtcgcgggg
    26881 cgcaccctgg acgtctccgg cgcgggctac gagcgtcacg gcgcgttctc cgatgcggga
    26941 cgctcgatcg cgccgccgcc gccgctgatg atgctgctgc aggccgccgc cctcgcctcc
    27001 gacgcgaagc tcaagcggca cggtgacgcc gagggctggt ccgtcaaggg cgatccgacg
    27061 gagctcgctc tgatcgtcgc ggcggagaag gccggacttc acaagcagca gctcgacttc
    27121 gagtttccgc gggtggccga gatcccgttc acgtccgaga cgaagaggat gacgaccgtg
    27181 caccggaccc ttcagggcga cgtcgcctac tcgaaggggg cgccggaggt catcctccac
    27241 ggctgcagcc ggttgctggg cgaggacggc gagcgcgccc tcggcgccgg ggacagggag
    27301 ctcgtcctcg gcaccgcgcg cgagatggcg agtcaggcgc tccgcgtcct cgccgtggcg
    27361 atgaagtctc gcgccggcct cgaggacgcc gagcgcgaga tgacgtttct cgggctggtc
    27421 ggaatgagcg acccgccccg tccggaggcc gcccgcgcga tcgagacgtg cgagcgggcc
    27481 ggcatcaaag caatcctgat caccggcgac cacgcgctca cggccgaggc ggtcgcccgc
    27541 gagctcggaa tgctccggca gggccgggtc gtgaccggga ccgggctgga cgcgatgagc
    27601 gacgaggatc tcgagcgcga ggtcggtgag atcgaggtct acgcgcgcgt ctcccccgcg
    27661 cacaagctgc gcgtcgtcac cgcctggcag cgcaagggcc actacgtggc catgacggga
    27721 gacggcgtca acgacgcccc ggcgctgaag aaggcggaca tcggcatcgc gatgggaatc
    27781 acggggaccg acgtctcgaa ggacgcggcg gcgatgacgc tgacggacga caacttcgcc
    27841 tcgatcgtag ccgccgtcga ggagggccgg ggcgtcttca gcaacatcaa gaagtacctg
    27901 atgtatctca tctcgtcgaa tgtcggcgag atcggtctca tggcgggagc ttccgtgctc
    27961 ggcctgccgc tccccctgac ggcggtccag atcctctacg tgaatctcgc cacggacggc
    28021 ctgccggctc tcgcgctcgc cgtggatcct ccggaggccg atctcatgag gcgccctccc
    28081 cgcgacccgc gggcggggat cttcacgcgc cccgtcctga cgctcacggt gctgggcggc
    28141 gtctggtcga tgctcgtcaa catcggcctc ttcgcgtggg ccctcggctc cggccgcagc
    28201 gtggccgagg ccgtgacgat gacgttcgtg tccctggtcc tgatccagtt cttcaaggcg
    28261 tacaacttcc gctccgaccg gaactccgtg ctcgagcggc cgttcgccaa caagtggctg
    28321 aacctcgcga tcgcatggga gatcgccgct ctcgcggcga tcctctacgt gcccgtcctc
    28381 aacaagccgt tcgggacgtt tccgctgccc ctcgtggact gggtcatcgt gacggccgcg
    28441 gccttcaccg tgtcgccggt cctcgagatc gcgaagtgga tggagcggcg cggctggttc
    28501 gggagcctcg ccgcgtgaat gtgcgctcga aggggtcccg cgacgcgggc cgtagtatcc
    28561 ggagatgagc ctcgaagcgt tcctgacctc gaccactctc gtggcggttg ccgagattgg
    28621 cgacaagacg cagctgctgt cgttcgtcct cgccgccaag ctccggcggc cttacgccat
    28681 catggggggc atcctcgtgg cgaccctggc caaccacgcg ctggccggaa gcgtcggcgt
    28741 ctggctggcc agcctggtcg cgccctcgac gctcaactgg atcgtcggcc tggcgttcat
    28801 cgggttcggc ctgtggacgc tccggcccga caccctcgac ggcgatcccc gggtgctcgg
    28861 agcgggcgcc ttcgtcacca cgacggtcgc attcttcctc gcggaaatgg gcgacaagac
    28921 acagctcgcg accgtggcgt tgggcgcgcg cttcgaccag ctcgcctggg tcgtcctcgg
    28981 cacgacgctg ggcatgatga tcgccaacgt tcccgcggtg atcgtgggcg aggccctggc
    29041 tcaccgaatt ccgatgaaga cggtccgctg gatcgcggca accctcttcg tcgcggccgg
    29101 gatcgcgaca ctcgcggggg tgggtgccga tcgaacccag ccgcgcccgc ctgagaaccc
    29161 gcccgaacgc cctccagcaa cctcgcccgt ccaatgagat cgggcagggg acaggaacgc
    29221 ccgtggtttc cggactctcg tgggctgtgg gaccgtgtgc ttcacgaaca tgtgtcgccg
    29281 gtgcgcattg ggctcgcggt cggggtcggc gttgtggtcg gttgcagccc gtttctcggc
    29341 cttcaggtcg cgatagcagc ggttctcgca atcgcgttcc gactcaacaa gctggcggtg
    29401 ttcatgggcc ttcaggtctc ggccccgccg ctcctgcctt tcgtactctt cgccggagcg
    29461 caactcgggg agctcgttcg gcacggccaa cttcttccaa tggacctgga cgcgctccgg
    29521 gcgatgccga caagagagat cgtccacatt ctcttcctcg acctcctggc tggaggcaca
    29581 ttgctcgggc tcgtgctcgg gacatcactc ggaacggtag ccgcgagagt tgcgatgcgc
    29641 agggcctcgc cggattccca cctctcccga ggcgagatgg cgcaaatggg agctcggctc
    29701 ccggccctgt cctttccact ccgtcagtac gtccggtgga agatcctctt cgacccggtc
    29761 tacgcgctgg tcgcttcgcg actgaagggt gtccgcgaga tcctcgatct cggagcgggg
    29821 ttggggatca tggccgccgt gctcgctcat cgggatccgg aacgacgtgt cgttatcgtc
    29881 gagtgggatg tacgaaaagt ggccggggcc agagcacttc tgtcaggact ttcgaagatc
    29941 gagatcgtct gcgccgatgc gcggctcttc ccgaccgctc cggtcgagtc gattctcctg
    30001 ctcgacgtcc ttcactacgt cgaaccgttc gctcaggacg cctggctcgg gcggtgtgcg
    30061 tctgggctga agccgggagg gcttctgatc atccgagaac atgacgcatc gcacaaaggg
    30121 gcggtggcgg aggggtttga gaggctggca gtcaagctcg gctggaaccg aggtcctggt
    30181 ttccatcctc ggaggacgat cgatctcgtc catcggctcg agagcctcgg tttggccgtg
    30241 gagacgatca aagacggtac tgcagacggt ctcactctgt tcgtcgcgcg caattcgtat
    30301 gcggcagaac gggagactgc tcgtctgtcc tccgtcttca gcaggggaat gaactaggcc
    30361 cggaatcccg gccgcagacg cgtcacgggc gccgcatttc gcggcgcccg tcacccaaac
    30421 cgggattctg gttgggtccg tctcgacgct tcaggacttc ttcgggcagg ccgggttcgg
    30481 gccggtgccg tccatcgggc ccgcctgcgc gccgttgccg tcacagcagg ccccgccggc
    30541 gcccctgccg tgtccggcgc cgcccatgcg gcccgcgaac acgggcgtgc cgtccgcgtt
    30601 ccggagcttc aggaccgttt tctgcgtgag gttctcgacc tcggcgacga cgaaaccctg
    30661 cgggcactcg acgcacgtgt agagggtgag gcgcaccgcg tctccggccg ccggcgcgaa
    30721 cttctgagcg gtgaggtagc ggtaagagcc gagcacgtag gcctcttcct tgccgccgac
    30781 cttcacggcg agcgtcggca tgccgagtcc cgggccgccc ttgagcgaga cgacgtctcc
    30841 gatcacggtg gagggcttcg tgacgtcgat ccttgcggcg gcagccgtgc ctgagccggg
    30901 gccgcgtcct gccggggaac cctgctgggc gaggacgggc agcgccgcga gagccgcgag
    30961 gagggcgagg gcggtgaggt tgcgtttcat ttcgatctcc ttccggcgaa agacaacgca
    31021 cgcctcgtgc cgactgccgc cgccgcgcac aagacgcggc cgctcaagcc tttcgaagaa
    31081 atctctcttc ccggcgtcct cggctccttg cagaagctgc agtccgcccg cagcggctgc
    31141 agtccggtca gttcaggccc cagcgcctga ggcggtaata gagcgtcctc accgagattc
    31201 cgagccgccg ggcggcgtcg tcccggttgc cgccggacag gcgcaaggcc gcgagagcgg
    31261 cctcgcgctc gcgcgtctcc tcgtcgagcg tgagcgccgg cgcggcggac gccgccgacg
    31321 gcttcacgtc ctcctcccgg atcggctggc caccccgcac cacgaccgcg cgctcgagcg
    31381 tgttgcggag ctcgcggacg ttgccgggcc agtcgtgccg cgcgagggcg tcgagcgccc
    31441 ccggcgtcag ctccggctcc gggagccggt gccgcgccgc gaggcgcgcc acgaagtgcc
    31501 gcgcgaggag cgagacgtct ccgggcctct cgcgcagagg cggcaaggcg acgggaacga
    31561 cggcgagccg gaagtagagg tcgcgccgga agccgcccgt ctcgacctcg cgcgcgaggt
    31621 cgcggttcgt cgcggccacg agacggacgt cgacggccac gtccttctcg cttccgaggc
    31681 gcctcaccct gcggtcctcg aggacgcgca ggagcttggc ctggagggcc ggcgcgagct
    31741 cgccgatctc gtcgagaaag agcgtcccgc cgctcgcctc ctcgaagcgc cccgcgcgcc
    31801 cgcggtccgc gcccgtgaac gccccgcgtt cgtggccgaa gagctcgctc tccgcaagcg
    31861 actcgggcag cgacgccgcg ttgaccgcga cgaacgggcc tgtcgcgcgg ggcgagcgcg
    31921 tgtggagccg ccgcgcgacg agctccttgc ccgtgcccga ctcccccgtc acgagcacgg
    31981 gcacgttggc cgcggcgacg cggtcgacca gcgcgaggac ctcgcgcagc cgcgcgtcgc
    32041 cggtgacgat ctcccccgca tccggttcgg gagcgaggag ccggtcgacg accgcgagaa
    32101 gcgcgcgggg agactcgagc ggtttcgtga ggtagtcggc ggcgccgagg cgcacggcct
    32161 cgaccgcctc cgggatcgtc ccaaagcccg tgaggacgac gacgggcggc ggcgacggga
    32221 gttccccgcg cagcgccttg aggagagcaa ggccatcgcg cgcgggcatc cgtcggtccg
    32281 tcaggaccag cgcgaaggcc tcgcggcgcg cgaagtggag cgcctcctcg ccgtcgcggg
    32341 cctccgtgac cgaatgcccc tctccttcga gaatgtcctt gaggagcgcg cggaaggacg
    32401 gatcgtcgtc cgcgacgaga atgcgcgcca tctcaggccc gctccccggg gagggtcagc
    32461 cgcgccacgc accctccgcc cgcgcgagcc tcgagcgtca cgtctccgcc gttcgcgcgg
    32521 gcgagcgccc tgaccgtcgg aaggccgagc ccggtgccgc cgggccggct cgtgacgtag
    32581 gggtcgaaag cgcgtgccgg atccaccgag aggccggggc cgcggtctcg cacctcgacc
    32641 caggcggtgc cgccggctct cccggcggcg atctcgaggg ctccgccggg atcgtgcgag
    32701 cgcgcgttcg ccaggagctc gtcgagcacg tcggccgcat ggcctccgtc cgcgaacgcg
    32761 cccacggccg tcgggccgga gaccgccacg gcgacccccg cgcgttccgc tgcctcgcgc
    32821 gcaagggcgg cgaggtcgag gctctggggc tgaggctcga cgggccgcgc gaacctcagg
    32881 atctgcgaaa cgagctcctc gatccgcgcg ctcgccgcga cgatccgccg cgagcgctcg
    32941 gcgacctgct ggccgggccc gtccaccatg agctgggccg tgcccttgat cgcggcgaga
    33001 gggttccgga cgcggtgcgc gagaccggcg cccgtgaggg cgaccgcatc gagccgctcg
    33061 cgctcggcct cgagccgctc gcgccgctcg cgctcg
//
LOCUS       DAOMFY010000014        31838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_441_length_31838_cov_8.604663, whole genome shotgun sequence.
ACCESSION   DAOMFY010000014 DAOMFY010000000
VERSION     DAOMFY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 31838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31838
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_31838_cov_8.604663"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLB01_02470"
     CDS             <1..253
                     /locus_tag="PLB01_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HQQ76193.1"
                     /translation="AREAGVAVEVSSAGLRKAVAEEYPSPPLLAGLVKAGVPIVFSSD
                     AHAPAEVAWGREAIEAAALAAGAREHLSFRKRERRRHPL"
     gene            complement(397..822)
                     /locus_tag="PLB01_02475"
     CDS             complement(397..822)
                     /locus_tag="PLB01_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQQ76194.1"
                     /translation="MVLLSRTIHFNAAHRLHNPGRSEEWNRATYGDSANAAGYGHNYA
                     LDVSVAGVADAATGMLVNLTDLDRILKEEVDRPLDHRNLNAEIPEFAKTVPTAENLAR
                     WIWDRVAARIAKEGWPCRLHTLRLTVTPNFSVELVEEKR"
     gene            complement(833..2167)
                     /locus_tag="PLB01_02480"
     CDS             complement(833..2167)
                     /locus_tag="PLB01_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HQQ76195.1"
                     /translation="MQSPVRRVVILGAAGRDFHDFNTVFRDKPGHQVVAFTATQIPGI
                     DSRRYPAALAGALYPDGIPIVPEDSLEHLIRTEKVDLCVFSYSDVAHTQVMHLASRTI
                     AAGADFLLLGADRTMLKSKVPVIAVTAVRTGAGKSQTTRYLSRILKELGLRAVAVRHP
                     MPYGDLAAQACQRFATYADLDREKCTIEEREEYEPHLDNGFVVYAGVDYERILRRAEA
                     EADVILWDGGNNDLPFYVPDLHICIADPHRPGHETAYHPGEANFRRANVILVNKCDTA
                     DEGNIHAVEMAAAKLNPKAHVLRANSPVVCDRPELVRGKRALVVEDGPTLTHGGMSFG
                     AGLVAAKKAGAARIVDPMPYAVGSLEATYRKYPNAAGILPAMGYGEAQIADLAETITA
                     TPCDVVVVGTPIDLTRVVRLAKPTVRVRYELEEVVRGRLAQEVAKVVPVHTV"
     gene            complement(2242..2661)
                     /locus_tag="PLB01_02485"
     CDS             complement(2242..2661)
                     /locus_tag="PLB01_02485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQQ76196.1"
                     /translation="MPPEEKTPSPVASAPKKEEKASKMIKTPSGLQYEDTVVGTGASP
                     KPGQTCVMHYTGWLWENEAKGKKFDSSVDRGQPFEFQIGLGMVIKGWDEGVSTMKVGG
                     KRTLLIPSALGYGSRGAGGVIPPNATLVFDVELLGVK"
     gene            complement(2793..3821)
                     /gene="fbp"
                     /locus_tag="PLB01_02490"
     CDS             complement(2793..3821)
                     /gene="fbp"
                     /locus_tag="PLB01_02490"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQQ76197.1"
                     /translation="MTTVGTRLNRFLYETERHYPSATGELSDLLSQLAFAGKRISRIL
                     SSAGLTEVLGTSGGVNVQGEQQQKLDALANEVFLEAFAYGQLVPTIVTEEMDLPAHLP
                     ENVESGKYIVFLDPLDGSSNLDVNGSVGSIFSVRRLSGRGEVTSEAELMSRISHEQVV
                     AGYFLYGPSTVLVYTEGRSVHGFTLDPGVGEFLLSHPEIRIPDHGPYYGANEGGIRDW
                     EEGPRRFVEEWAREKDEVSGRPYSTRYSGALVADFHRIVLKGGIYLYPATAQSPQGKI
                     RLMYEAAPLALVAEAAGGAASDGRRRILDIVPTRNHGRTPIYLGSKDDVARVDGFVRE
                     SKAVAARG"
     gene            complement(3818..4567)
                     /gene="pgl"
                     /locus_tag="PLB01_02495"
     CDS             complement(3818..4567)
                     /gene="pgl"
                     /locus_tag="PLB01_02495"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQQ76198.1"
                     /translation="MSDVRPFATADDVADAAVFLFVTLAAKAIAERGEFFCSLAGGTT
                     PLAGYRILAAPMISSKVNWERTHVFWGDERCVPEGDKGRNDEAALDALLRKVPIPSKN
                     IHRVPATEPDGAQRYESELRRVFSSSFRESPSSSEIPRFDLILLGLGQDGHTASLFPG
                     HPAVEEKTRLVVRVDGAPKPPPSRVTFTLPLINAARHVAFLVTGKDKNAALRRVLNGD
                     PSLPAARVAPVDGTLAFLSDAAALTGEEAPS"
     gene            complement(4564..6066)
                     /gene="zwf"
                     /locus_tag="PLB01_02500"
     CDS             complement(4564..6066)
                     /gene="zwf"
                     /locus_tag="PLB01_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HQQ76199.1"
                     /translation="MSAATPEPATIVIFGGSGDLSKRKLVPALFELDAQGQLSPGTCI
                     VGYARTGESDETYRAEMKQAVAEFARVKPVDEAKWRTFESRLFFFRGDLNSGKSFADL
                     KTRLEALEKERGTPGNRMFYLAIPPSMIGPVVKNLGDAQFVYPVDGGPWSRVIVEKPF
                     GRDLATARALNEELSRVFRERQIFRIDHYLGKETTQNILVFRLGNGIFEPLWNRRYVE
                     HVQITVAESIGVENRGRFYEESGVLRDIVQNHMLQLLCLVAMEPPVAFDPNAVRDEKV
                     KVLKCIQPQTPEEIIRDTVRGQYGPGAIGGQPVVGYRSEKDVNPMSLRDTYAAWKVQV
                     ENWRWAGVPFYLRAGKRLAKRVTEIAIQFKTPPISLFRQVGVVRQEPNALILRIQPDE
                     GIALKFGAKEPGPTMKVDPVTMDFRYSEFFGASQPEAYERLLMDAMHGDSTLFARRDE
                     VEAAWERVTPLLDAWRENPPLDHPNYEAGTWGPDAAVALLARDGRRWRRP"
     gene            complement(6074..8017)
                     /locus_tag="PLB01_02505"
     CDS             complement(6074..8017)
                     /locus_tag="PLB01_02505"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQQ76200.1"
                     /translation="MILGTFEAPELADAQVERLAELGAAARGDILRMTTLAASGHPGG
                     SMSSIDFELVLWGFANCDPKNPFDPKRDRIVISHGHTSPAAYAALAHLGWFDRALPLV
                     GFRRAGSPFEGHVERDVPGIEWGTGNLGQGLSAAAGFALAAKIKGDGSKVFCAMGDGE
                     QQKGQLAEARRFAVKYGLTNLVGVLDWNREQLSGSNDAILRQDIVADWEADGWGVIEV
                     DGHDVRAVYRALRAALRAEKPTLVAAHTVMSKGVPFMEKDGFKWHGAALAPEKCREAL
                     AILGVEDDLDRWVEERKKPAPDWKKALPHRPEESVVLASGGTPRTYPLDKQTDNRSAF
                     GNALADLASTAAAAGTPFAVIDCDLSVSTKTETVEAKVPSAFFQGGIAEHNAAVLLGA
                     LSVAGVHAWWGEFAMFGIAETYNQQRLNDINGANAKLAVTHAGIDVGEDGKTHHSIDT
                     FGLLNSTFGWKVFTPADPNQTDRVVRWMATHRGNHALVMGRSKIPFALKEDGTAFFAG
                     EAAFDPSRADVLRSGDGPLALVSAGNMLPHALDAWNALAQEGVRADLVSVCAWSDLAD
                     EDLAFMARHGLVVSVEDHNPKTGLGTLLQARFNDLGLPARVVKLGVTGYASSGPAKEL
                     YRRMGLDGAGIAAAVRAALAGKR"
     gene            complement(8014..9153)
                     /gene="tal"
                     /locus_tag="PLB01_02510"
     CDS             complement(8014..9153)
                     /gene="tal"
                     /locus_tag="PLB01_02510"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HQQ76201.1"
                     /translation="MSAPIAYRRRGQAVWLDSISSKMIRTGRLKSLVDAGTIYGVTSN
                     PTIFGQAIEKNEGDYNAGIERLARAGKDAFAIYDALTQQDIAEGADAFRAIHDRNPVD
                     GWISLEVLPSLARDTEKTVGEAKRLAAALGRPNVFIKVPATPEGVPAIRRLIGEGISV
                     NVTLMFNRRHYRDVALAYLGGLEDLAAKGGDLSRVHSVASFFVSRVDTLIDRRLDEKA
                     AAASGAGKAHLESLRGRAGLANSKVVYQDFRDIFGAERFTSLRGKGARLQRPLWASTG
                     TKNPAYSDLLYVENLVGPDTVNTMPEKTLDALLDHGSATPSDTVLEGVAESRTVLSEL
                     AGIGIDVEAVGEELQKDGVVLFEKSYDQLLGTIEQRRQAAAGVKA"
     gene            complement(9181..10335)
                     /gene="hflX"
                     /locus_tag="PLB01_02515"
     CDS             complement(9181..10335)
                     /gene="hflX"
                     /locus_tag="PLB01_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQQ76202.1"
                     /translation="MRKSGAEEPAEAQPPPDSNKTILVGVYGREVPRALAEDHLDELE
                     RLVDTAGGVVVARALQERRSPDPATYVGSGKLEEIAEVARAQGAGWTIFDDELTPSQS
                     RNLEKTLPTNVLDRAGVILRIFAARARSREAMTQVELASLQYQLPRLAGKSQGLAQQR
                     GGGAFRGGAGERKIELDRRRLRMRIAKLKEELGKIETQRDVRRKGMKRVATVSLVGYT
                     NAGKTTLFNRLTSSKEFAEDRLFATLDPRHARLHGVGGRAIVLADTVGFLRKLPHDLV
                     ASFKSTLAEVRDADLLVHVVDASSPAASEQKAVAEQVLEELGVEKRRVLLAYNKTDRP
                     SPEALDADGLRISAATGAGLPELREAIVARLSALGARLHVYGVHESETTS"
     gene            complement(10475..10849)
                     /locus_tag="PLB01_02520"
     CDS             complement(10475..10849)
                     /locus_tag="PLB01_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76203.1"
                     /translation="MNVGLGRGVGVAVAAGVGVAELTGVGVDVGVAVDVAIGVAVDVG
                     VGVAVDVGVGVAVDVAVAVAVDVAVAVAVDVGVAVAVGVVVPNEVGVAVGVGVGVEVA
                     GGVEVVVGVLVGVNVGPWASAW"
     gene            11002..15273
                     /locus_tag="PLB01_02525"
     CDS             11002..15273
                     /locus_tag="PLB01_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HQQ76204.1"
                     /translation="MKVLGREARRTGVLIAAVIVAAAAFRLFRLDWDARQHLHPDERF
                     LTMAETALSWPETLSTYFDEAHSTLNPRNVGFPFYAYGTFPIVAVKAVASALHRTSFD
                     EVTLVGRAVSAVADLATLALLLLLAWRVSRDRRVVLLAGFFYAFAVLPIQQAHFFTVD
                     SLATCLATAVLLSLVAVQSSPRPARFAWAGALIGLALATKITSALLVLVAAVVVAEAV
                     LRAWKRGGAPRGAPVESLLFRSTLAGIAAFSVFRLAQPDAFRGPGLLGILPSDRWLSN
                     FAEAAALVNGSRDIPPGVQWAGRVMLAWPLENLVLWGLGVPLGLAALCGTLAAAAAVQ
                     RRREGALLVPVAWVAVVLVYQGTQWVASMRYFLPAYPALCLLAAWGLVRLRDRALTTA
                     ARRAAAGAIVLTGIGTLLWATAFMRIYTRPHPRIEASRWIYANVSPGATIACEHWDDP
                     LPLRLSGPDPFPSLYTGFQLPWYDDDTPKKLTKVLDGLDHADVLVLSSDRLSGSIPRM
                     PLRWPMTTRYYEALEDGSLGFEEAARFTSRPGLLGLSVPDERAEEAFTVYDHPRVRIF
                     RKTPAYSHDRAARILDVDLGRILRMTPREASRAPNGLMLSEVAWERVRHRGTWSSLFG
                     PAAPGAFRSVILWSLLLLVLGLVGAPLTALACRSLPDGGFAFARAVGLLATSWIAWLV
                     ASLRIAPFSSGTLIVALGILGAVSAAVAVARRRELKALLAERGRLLAAEELLFWSAFV
                     AFLLIRRANPDLWHPTLGGEKPMDLAFLTAVVKSEDFPPYDPWFAGGALNYYYFGFVL
                     AGALVKLTGVAPAVAYNLAVPTFFALTVAGAFGAALALVAPARGGAGRRTAGFAILGA
                     LFVAVMGNLGEVRLYLEAARSASPSTWASPVPGLTALVRTATGLLASARAGTPLVAHP
                     DWAYWTATRLIGHAEAEPGPINEMPFFTYLFGDLHAHALALPLTLLVFGLAAAHALAE
                     AGESRLAEGARLALLALGLGALVATNTWDAPTYGLLVFVACVIGAFARGDSAASQLRG
                     AFMRAGLVAVGAAALFWPFLRSWGRPYGGFSAWTGSSTSLGAFLLIHAIFLFVIVLAF
                     ALELSQPGNREALRNFATRGSPAIAFARNATGVAAFLGGILVLLGRPVAGVLVGLLAL
                     TAVVSLCSGHDASWRLTLAGAATGFGVLLSAELIVLTGDVGRMNTVFKFSLQAWVLLG
                     LASAACAARIAAVLPGLGPAIRHAFRSAFALLLFGGLLYPLLATPARIRDRFDPAGAR
                     GLDGSSFLRNAVIDMGGARTPLDWDRQAFVWIQENVSGTPVVAEANTDPVLYGWGNRY
                     AMFTGLPSVIGWRWHEVQQRAATGAEAIDRRIADLGRFYRTEDLDEAARLARRYNVEL
                     VVVGPLERAIYGNTGLGKFAAGGRFTLVYENPGVRLYRVNPGPRS"
     gene            15270..16061
                     /locus_tag="PLB01_02530"
     CDS             15270..16061
                     /locus_tag="PLB01_02530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQQ76205.1"
                     /translation="MTGLASRLRYYASSLVTLAAGVRNPLAVLLALATGRKPFLLELA
                     NGLRFLVRGPLDVWIVKETCLDGEYDAWAPAPGACSVVFDVGAGLGDFAVHAARRWPE
                     ARVFSFEPHPETFSLLERNLALDGVTQVVAVRAALTGAGTAEAVLREGHSASERSTAT
                     GSGPGIAVPSTTLGRAFETHRIERCGFLKIDIEGAEFDVLLSADEATLGRVEALCLEW
                     HEGPGRTIEALASHLSACGFAVRLRRSPVHAHLGLLDARRAAELP"
     gene            16058..17203
                     /locus_tag="PLB01_02535"
     CDS             16058..17203
                     /locus_tag="PLB01_02535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQQ76206.1"
                     /translation="MRILSVLTYYSPHWTGLTRHAVRVAEDLARRGHEVTVLSTRHTP
                     DLPPHETQGGVRIVRVEPVSRFSRGMFAPMFPLAALRLMARTDVVQIHSPLPEAPLVA
                     FFGRLLDKPVVLTHHADVVMPAGLANRFVEHAALAVLTSAARLASAVTSYSRDYADHS
                     PFLSAFEDRLTCIAPPVRLPEPDRIAARAWRDSFAPHTRNLLGFAGRWVEEKGFDVLL
                     RAFPLVLRELPDAHLVYAGESGVVYENSFDRCRPLLEPLRDHVTFLGLLRDPALMANF
                     YALCDLFVLPSRSDMMALVQVEAMLAGTPVVASDIPGARIVVERTGFGRLAPPEDPEG
                     LARVLVETLRDRERFRPEPGAVAAVFNPQTSIDDYEALFHRVLGDAR"
     gene            17200..18051
                     /locus_tag="PLB01_02540"
     CDS             17200..18051
                     /locus_tag="PLB01_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ76207.1"
                     /translation="MTLTPGDRAALDRLLAREWDFAFARRTRLLFEDLDPRPGERILD
                     CGCGIGFHLLLLQAWRGVAPSGIDMDVGRLRKANRGGARPRVAGAALPRLPFRDGTFD
                     KVLASEVLEHLPDDRAALAELFRVLKPGGILALSVPHARFPFWWDPISRVWGALGGNP
                     IRRGPLVGIWTGHERLYLPENLAARASEAGFRVERCVEATTHAFPFSHFILYGIGKPL
                     LESGLLPRSFSEGVGRLSTSSAADVGWSSFLRGALARVDARNEVAPAAADRFVNIVLR
                     ARKPEGT"
     gene            18105..19898
                     /locus_tag="PLB01_02545"
     CDS             18105..19898
                     /locus_tag="PLB01_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76208.1"
                     /translation="MALSAAAWFGTAGNQFSGRGFFAWAASLVILWRLLDRPGPPHPP
                     AFPISRATGAGLVVILLGATALRFAALSSYPPDMTSDHVEKLLDVQRILDGDRPVFMG
                     NNGGRESLHFYGLALLAKATGQPLDFDLLKAGSGLVGLLGVLASFWLGRILAGDDGSA
                     FADGTGLLCAALVSVSWWHVMLSRLGLRIVWTPFFVCLLAGFLARALRTGRRRAYLAA
                     GLTVGFGLYAYQALRMAPVFVTLGGLAALFLARPAGGTRAMAAGNFAALVALAAATAV
                     PLARYAHDAPAVFWARTKGRILGDDAVSAADRHAALRSNLSRLGSNAARAAGMLHVSG
                     DRAWITGAPQGPPQVDRLTGVLLAAGFAGLAVRLVRRRDPGDALVLLGLPVMLLPTAL
                     ALARPSEVPSATRASGALPFALFLAAWAGAHAIRLFARRAGAAPAVSIVALGLAVSAP
                     ANAHIYFGPAMDAYRSSTFPYAEAGAVLGAFGRVHGAPGNAFMVGAPNWWDHRALAFT
                     SGFNRWDNGVLPDPLFGRLDEKVRTNAGTPLAVRPEAPLLFFVSPDDTGTLDRLRTKF
                     PEGRLRRIHTSRPGRDYLLLYVDSPLLRSGP"
     gene            19906..20847
                     /locus_tag="PLB01_02550"
     CDS             19906..20847
                     /locus_tag="PLB01_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HQQ76209.1"
                     /translation="MTRGAGPRDLPLLGERLDALRADWAERRLDSDPLEFARRAKPVE
                     QEVTAFLAASLAFGRVASIRTSIRRILDALEDRPAAFLERWDERPIPALEHFRHRWVS
                     GEDVEDFLRMVKRARQAHGSLGALFAAGDEEEEKIFLEGEEGLPASRAAFPDYTAALS
                     HFHRNLLSFSSSSTPSRGLKFLLPEPRSGSACKRSHLFLRWMVRTEGFDLGLWTGGSF
                     TPARLLLPMDTHVHRIATYLGLTRRPSADLAASREATGWLRRLNPEDPVAYDWALSRL
                     GILAECVIERTRRHCERCAVRPVCRASLIPSQGAAAA"
     gene            20844..21428
                     /locus_tag="PLB01_02555"
     CDS             20844..21428
                     /locus_tag="PLB01_02555"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQQ76210.1"
                     /translation="MNDFLDRRKALSLGIAALVSAAIAFVARDALRPPAVRAPAPAAP
                     EKTAVLPAGPLVVRDRQGAARDLAVKSGRGLILHFWATWCGPCREEMPGLVKFVKETK
                     GDPNVEFLAVSVDEDWKVVDAWLKERGIADLPLALDPKGDTARRVGTKLFPETWFVAP
                     SGRIFHYIDGPADWNDPRLRALAAEFSRASAARS"
     gene            complement(21425..22219)
                     /locus_tag="PLB01_02560"
     CDS             complement(21425..22219)
                     /locus_tag="PLB01_02560"
                     /inference="COORDINATES: protein motif:HMM:NF026037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76211.1"
                     /translation="MQASLLVAAVVFFLEGNRGGGARAEGGAAAAGSGASPSSPSKKS
                     ESAVSGAPHPEARDGVFGVVTLSGPVPKLAPRPVISDVAICGRADRPSPALVLGKGRE
                     VGNAFVWLEGATGGDLPKGPFRIDQKGCAFEPYAQVVSRGVPVTVLNSDPVLHNVHAT
                     TVKGDALFNKAIPVKGQSFTERFTTAGVVRIKCDVHAWMNAWLFVADGPFAAVTDREG
                     RFAFPGVAPGTYRLHAWHELLGEKVQSVVVPASGVARADVTLSLAD"
     gene            complement(22241..25357)
                     /locus_tag="PLB01_02565"
     CDS             complement(22241..25357)
                     /locus_tag="PLB01_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQQ76212.1"
                     /translation="MAKAPPPEPIDHHYDIAKLTKVFAIVSILALPAFAWMTWQDYGR
                     DWKSWQKKFVENDRKKTREALRAAAERIDPEAEQKYLDQKREGARELRRNRAAVAEAE
                     ERDRKAEGAWYDADQDFRFKKAEMDTARYVFETRQKEDPKSAATAKAKAAYERLRAAY
                     EESRGREQAAQKAWDGTRTALAELQKTKKDAEEALGKLDEEYDRYRAKLKTLKQDDAF
                     YFVRNAPIADMLAPSLKIQQVQLDGLSNDVNFLRSQRVDRCVTCHIAAEKRGFEDKQK
                     YPESVLRTHPRLDLFVDSNSPHPYATFGCTSCHGGRDRATDFTRAGHMPNDYAAYEKL
                     EEERHAGRDEKGMPVDDDALRELMEETQTGRWMKAYGWEVDRFNDLPMYPMKAVEAGC
                     LRCHRQDAGHPKAAKLDQGRKLIEALGCWSCHKMRGIEDLPKPGPNLARVAAKTTPEW
                     TGRWLVNPRAFRSNTKMPRFFYLENFDTPSGHKLTDTMVAGVTAYLFEKSGKAAYPAP
                     AKGDAARGKVLFENVGCQGCHISEPGAERRVMDGWRQHGPNLIGLSEKTTPGWIAAWL
                     KDPKAYNPETRMPNLRLADDEISDLVAYLWSRPSQPEFKASHAPKADPAERDALVSEY
                     LMQTRSVVDAKADLAKMSAHDKDLFVGEKIIAHYGCYACHNIPGFEKAKPIGVELSEW
                     GNKPVHLLDFGFVSVPHTRADWLAQKVGQPRSYDREKVKSFQEKLKMPQFSLSPAEIE
                     AVQSTVLGMQKDDMSDALTARATGPRGAIEAGRRIVKDYNCQGCHVLEDKGGAIREAM
                     TASKIDLAMAPPIIRGEGAKVQSEWLFAFLHGPKTGQIRPWLKVRMPTFGFTTEQINA
                     LTAYFAAQDKARYPFDGRVEALDPRSRAAGAATFASLKCAQCHPTSQDAFAQALREGK
                     TPADLAPILAAAHTRLRYDWIADWIKRPDEWMPGTRMPTNFPKVDDKSDKRISPLAPA
                     LDTPAFAGLKKDLVAIWGSEAEAKAYLGDPERVTKALRDHIWSLGNDFQTAPSAPRKK
                     APTQVASH"
     gene            complement(25359..26435)
                     /locus_tag="PLB01_02570"
     CDS             complement(25359..26435)
                     /locus_tag="PLB01_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76213.1"
                     /translation="MNAIKHAINWLASPAIAVTLSMVGLVSAMFYRPLWTKRGGLLLF
                     ATLVGGFAFSFTDPNFRIVASMPDNVPILGMLFLVGFFFWYAMYQAHLNDARLEKGLP
                     PLEKEDSDKKVFTWPDLVYVELIALIVCSIVMTVWSIVLKAPLEEPANPGAAPNPAKA
                     PWYFLGLQELLVYFDPWMAGVVLPTLIIVGLMAIPYIDTNPRGNGYFSFRERKWEILM
                     FCFGFIILWVVLIILGTFLRGPNWNFFGPYETWDPNKLEPLNNVSVSEIFWVKWLNTA
                     LPTFWLKREFPGIFLVLFYMGALPGLLSKTLFRRFYEKMGTVRYLVGVNLFLIMLAVP
                     IKMYLRWIFNLKYIVNIQEFSFNI"
     gene            complement(26439..27233)
                     /locus_tag="PLB01_02575"
     CDS             complement(26439..27233)
                     /locus_tag="PLB01_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQQ76214.1"
                     /translation="MSLEHRWRKLQRAVVETQIWKSIFRVGIPNTNRKRVLAVLGNVV
                     LHLHPVKTRKSGVKMRYTWCAGGMTFFLFLVLTFTGLLLMFYYRPTIEYAYGDMLDLR
                     EQVPFGVMREMHRWGAHAMVIAVWIHMFRVFMTGSYKPPREFNWNVGVVLLVLTLLLS
                     FTGYLLPWDQLAIWAITVGSNMARATPLAGYEGPFAAQIAVNGVNLVTAGSDARFGLL
                     GGRIVGANALLRFYVLHCVFIPLIAAFLMAVHFWRVRKDGGISGPL"
     gene            complement(27281..27832)
                     /locus_tag="PLB01_02580"
     CDS             complement(27281..27832)
                     /locus_tag="PLB01_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQQ76215.1"
                     /translation="MADTVTTGTAPAATTATEAPSPSRRGFLSWLAVAWLAFAAGVGA
                     LLTLTARFLFPNVLFEPPSSFKAGYPSDYQAGEVDERWKEKYQVWIVRNTEMLYALIT
                     VCTHLGCIPNWLAAQNKFKCPCHGSGYYRSGINFEGPTPRPLERAAISISPDDGQIFI
                     DKSRKFLYEKGQWTDPAAFIKTT"
     gene            complement(27907..28815)
                     /locus_tag="PLB01_02585"
     CDS             complement(27907..28815)
                     /locus_tag="PLB01_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQQ76216.1"
                     /translation="MTERTPPPGQPHEPFEESSSSDSVSSATPAGRLLVQFFLIPAFV
                     VGVAVFVFWLFGSLAVDHKTPVELLADVRTGGRNQRWQAAFELTKKLPALTDPAEKAA
                     FSAAAIKAFEGAGSDDPRVRRYLTLVLGRLGDPKSVPLLESALDDKDAETRLYALWAL
                     ALVGDPKSAAKVRPLLDSEDPGVRKTAAFAAGRMEDGDAVPALKRLLGDPVTDVRWNA
                     ALALATLDDASGKDVLLGMCDRATLRATPGLSAEQQEDALLNGLRGLALLKDPDAQAL
                     GKRMVEADPSLRVRREARKILESPRR"
     gene            complement(28888..29355)
                     /locus_tag="PLB01_02590"
     CDS             complement(28888..29355)
                     /locus_tag="PLB01_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ76217.1"
                     /translation="MSPGRDMPHFIHDTCIGCGVCEIKCPTHTITGGKNEKFVIHQNR
                     CIDCSVCAVYCPVSCIQDQHGEIVQKVKPKDIPKAIVDRDLCTACEFCIDICPFDCIH
                     LVADPLAPNFHYKVAEVDAAPCVSCRLCETVCDKDAIYVPNPITQAKQYLPVL"
     gene            29483..30061
                     /locus_tag="PLB01_02595"
     CDS             29483..30061
                     /locus_tag="PLB01_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76218.1"
                     /translation="MKELRSAVNALFLFAIFLLVYSAVVPGHARGSVRGPRFLRMQVE
                     DNHQPGHPVKVSLSVPYGFVSGALRFASLGKVRRELDLHFDESVDASEVRTIVQELKD
                     KPEGTDVVRTHDDATLTLRKEGGLVTMEVRKKDRDKETVTLKLPWRLVDALAEGDRDL
                     DVNGLVSQLRDARRGDLLEVTAPDAHVKIWVE"
     gene            complement(30068..30607)
                     /locus_tag="PLB01_02600"
     CDS             complement(30068..30607)
                     /locus_tag="PLB01_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76219.1"
                     /translation="MFEPAPLPPTPDLPALTVRWEGLVVAIDATTLNTFARQLIRNVH
                     EVREVLIDPEDGRLGITVRVKKGIPVAFRGELRDMRVKDGYLGFAIADLRVFKRVPIP
                     NWVVKKIVDMQPPGRAFFYPEDRIVVVDLSGVLPPELSVQVKDVVCENGEMRLVFGPS
                     QYRLDKLLADMGRDPFADE"
     gene            complement(30629..31612)
                     /locus_tag="PLB01_02605"
     CDS             complement(30629..31612)
                     /locus_tag="PLB01_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HQQ76220.1"
                     /translation="MTLKTAIRLTRPFTLLPPLLGILSGAVCAFGSVHNPDPAHRVTP
                     ALLGIVALGALCASLLNAASNVLNQITDLDVDRINKPDRPLVTGEASIASAWRLTWTL
                     YALAMVPVWWIVVPAVSGFAPRALAPLPTHQCFFLFLGGLLATLVYSVPAFGRTKRLG
                     IWANVTIAVPRGTLLKVAGWTLVASAFSAEPWVIGGLFGLFLLGASSTKDFSDAEGDR
                     AGGCETLPVKYGNRRAVAMISPFFVLPWLLLAGLAWVGDPFAPGHRLLSGNPWLLTAL
                     GLLLAAWGAYTVHLLSRNPDELATTENHPSWTHMYLMLMCAQVGFALAYVL"
     gene            complement(31609..>31838)
                     /locus_tag="PLB01_02610"
     CDS             complement(31609..>31838)
                     /locus_tag="PLB01_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76221.1"
                     /translation="AAGLLAWVGVAAFVLAPSGAAAVVLAALLGTILESLLGRAGARW
                     RVSNGHVLNFVNTLAGAAAAPSLAALLGGIS"
BASE COUNT         4472 a        11273 c        10794 g         5299 t
ORIGIN      
        1 cgcgcgggag gccggcgtcg ccgtggaggt ctcgagcgcg gggctgcgga aggccgtcgc
       61 cgaggagtac ccctctcccc cgctcctcgc gggcctcgtg aaggccggcg tcccgatcgt
      121 cttctcgtcg gacgcccacg cgcccgccga ggtggcctgg ggccgcgagg cgatcgaagc
      181 cgcggctctc gccgcgggcg cgagagaaca tttgagtttt cggaagagag agagaagaag
      241 acaccctctc tgaaattcag gtgtccaatc gacgggaaac ggtctgcaaa ttccttggaa
      301 gggtgaatgg ccttggagcc gctcgagcca ccgcgcggcg ggcgcatttg ccgcggcccc
      361 acacacacca tctaagaaat cttctcttct ccccctctat ctcttctctt caacgagctc
      421 gaccgagaag ttcggcgtca cggtgaggcg cagggtgtgg aggcggcagg gccagccttc
      481 cttcgcgatg cgcgccgcga cgcggtccca gatccagcgt gcgaggttct cggcggtcgg
      541 gaccgtcttc gcgaactccg ggatctcggc gttcaggttc cggtggtcga gcgggcggtc
      601 gacctcctcc ttgaggatcc ggtcgaggtc cgtgaggttc acgagcatcc cggtcgcggc
      661 gtcggcgacg cccgcgacgg acacgtccag ggcgtagttg tggccgtatc cggccgcgtt
      721 ggccgagtcg ccgtacgtgg cgcggttcca ctcctcgctc cggccggggt tgtggagccg
      781 gtgcgccgcg ttgaagtgaa tcgtccgcga aagaaggacc atcgaccccg gactatacgg
      841 tgtggacggg gaccaccttc gcgacctcct gggccagccg gccccggacg acctcctcga
      901 gctcgtaccg cacgcggacc gtgggcttcg cgagccggac gacgcgcgtg agatcgatcg
      961 gcgtcccgac gacgacgacg tcgcacggcg tcgcggtgat ggtctcggcg agatcggcga
     1021 tctgcgcctc tccgtagccc atcgccggca ggatccccgc cgcgttcggg tactttcggt
     1081 acgtcgcctc gagcgagccc acggcgtagg gcatcgggtc gacgatgcgg gccgcgcccg
     1141 ccttcttcgc cgccacgagg ccggcgccga agctcatgcc gccgtgagtc agcgtcggcc
     1201 cgtcctcgac aacgagggca cgcttgccgc ggacgagctc ggggcggtcg cagacgaccg
     1261 gcgaattcgc gcggaggacg tgcgccttcg ggttcagctt cgcggccgcc atctcgacgg
     1321 cgtgtatgtt gccctcgtcc gccgtgtcgc acttgttgac gaggatgacg ttcgcccgcc
     1381 tgaagttcgc ttcgcccggg tggtaggccg tctcgtgccc cggccggtgc ggatccgcga
     1441 tgcagatgtg gaggtccgga acgtagaacg gcaggtcgtt gttgcccccg tcccagagga
     1501 tcacgtcggc ctcggcctcg gcccgccgca ggatcctctc gtagtcgacg cccgcgtaga
     1561 ccacgaagcc gttgtcgagg tgcggctcgt actcctcccg ctcctcgatc gtgcatttct
     1621 cccggtcgag gtcggcgtac gtcgcgaagc gctggcaggc ctgcgccgcg aggtcgccgt
     1681 atggcatcgg gtggcgcacc gcgaccgcgc ggaggccgag ctccttgagg atgcgcgaga
     1741 ggtagcgcgt cgtctggctc ttcccggcgc cggtgcggac ggccgtgacg gcgatgacgg
     1801 gcaccttcga cttcagcatc gtccggtccg cgccgaggag caggaagtcg gcgcccgcgg
     1861 cgatcgtgcg agacgcgagg tgcatgacct gcgtgtgcgc gacgtccgag tacgagaaga
     1921 cgcagaggtc gaccttctcc gtcctgatca ggtgctcgag cgagtcctcg ggcacgatcg
     1981 ggatgccgtc gggatagagc gcgcccgcga gggccgccgg ataccggcgc gagtcgatcc
     2041 cgggaatctg cgttgccgtg aaggcgacga cctggtggcc cggtttgtcc cggaagacgg
     2101 tgttgaagtc gtggaagtcg cggccggcgg cgcccagaat gacgacacgc ctcacggggc
     2161 tctgcatgaa gaccctccgc ggggaaaccc ccgcactcca aatctagggg ccggccccgc
     2221 ggaaggcctg attccgatgc tctacttgac tccgaggagc tccacgtcga agacgagcgt
     2281 cgcgttcggc gggatgacgc ccccggcgcc gcgagagccg tagccgagcg cagacggaat
     2341 caggagggtg cgctttccgc cgaccttcat cgtggagacg ccctcgtccc agcccttgat
     2401 gaccatgccg aggccgatct ggaactcgaa cggctggccg cggtcgaccg agctgtcgaa
     2461 cttctttccc ttggcctcgt tctcccagag ccagcccgtg tagtgcatca cgcaggtctg
     2521 cccgggcttc ggcgaggcgc ctgtgccgac gacggtgtct tcgtactgga ggccggacgg
     2581 agtcttgatc atcttggacg ctttctcctc tttcttcggc gccgaagcaa cgggcgaagg
     2641 tgtcttttct tcgggtggca gagcgaacga cgccggcgcg gcgaggaagg tcacggcgca
     2701 aaccgccgct aggtttctga acgaaagttt cacgcgatct cctttcaaag aaatcttcat
     2761 ccccggttct ccttctctct gttctttcgc cttcatccgc gcgccgcaac cgccttcgac
     2821 tcgcggacga acccgtccac gcgcgcgacg tcgtccttcg acccgaggta gatcggcgtg
     2881 cgcccgtggt tccgcgtcgg gacgatgtcg aggatgcggc ggcggccgtc gctggccgcg
     2941 ccgcccgccg cctccgccac gagcgcgagg ggcgcggcct cgtacatgag gcggatcttt
     3001 ccctgcggac tctgcgcggt cgccgggtag aggtagatcc ctcccttgag gacgatccgg
     3061 tggaaatcgg cgacgagcgc acccgagtag cgcgtcgaat aagggcggcc cgagacctcg
     3121 tccttttccc gggcccattc ctcgacgaag cgccgggggc cctcttccca gtcccggatc
     3181 ccgccctcgt tcgcgccgta gtacggcccg tggtccggaa tgcggatctc ggggtgggac
     3241 aggaggaact cgccgacgcc ggggtccagc gtgaagccgt ggaccgagcg cccctccgtg
     3301 tagacgagca ccgtcgaggg gccgtagagg aagtagcccg cgacgacctg ctcgtgggag
     3361 atgcgcgaca taagctcggc ctcggacgtc acctcgccgc gcccggagag cctgcgcacc
     3421 gagaagatcg agccgacgct gccgttcacg tcgaggttcg acgagccgtc gagcgggtcg
     3481 agaaagacga tgtacttccc ggactcgacg ttctccggga ggtgggcggg caggtccatc
     3541 tcttcggtca cgatcgtcgg gacgagctgt ccgtacgcga acgcctcgag aaacacctcg
     3601 ttggcgaggg cgtcgagctt ctgctgctgc tcgccctgga cgttgacgcc gcccgacgtg
     3661 ccgaggactt ccgtcaggcc cgccgacgag aggatccggc tgatgcgctt gcccgcgaat
     3721 gcgagctggg acaggaggtc cgagagctcg ccggtggccg acgggtagtg gcgctccgtc
     3781 tcgtagagga agcggttcag tctcgtcccg accgtcgtca cgagggtgcc tcctcccccg
     3841 tcagggctgc ggcgtccgag aggaacgcga gcgtcccgtc gacgggcgcg acgcgggccg
     3901 ccggcagcga cggatcgccg ttcaggacgc gccggagcgc cgcgttcttg tcctttccgg
     3961 tcacgaggaa ggcgacgtgc cgcgccgcgt tgatgagcgg cagcgtgaac gtgacgcgcg
     4021 acggcggcgg cttcggcgcg ccgtccacgc ggacgacgag gcgcgtcttc tcctcgacgg
     4081 ccgggtgccc cgggaaaagc gacgccgtgt gcccgtcctg cccgagacca aggaggatca
     4141 ggtcgaagcg agggatttcg gaagaagagg gagattctct gaatgaggaa gagaagaccc
     4201 ggcgcaactc ggattcgtat cgctgcgccc cgtcgggttc ggtggcgggg acgcggtgga
     4261 tgttcttcga agggatgggg acctttcgca ggagcgcgtc gagtgccgcc tcgtcgttcc
     4321 ttcccttgtc gccctcgggc acgcaccgtt cgtcccccca gaagacgtgc gttctctccc
     4381 aattcacttt cgaagaaatc atcggtgcgg cgaggatgcg atagccggcc aggggagtcg
     4441 tgccccccgc gagggagcaa aagaattctc ccctctccgc gatcgccttc gcggcgagcg
     4501 tcacgaacag gaacaccgcg gcgtcggcga cgtcgtcggc ggtcgcgaac gggcgaacgt
     4561 cgctcacggg cgccgccagc gcctcccgtc ccgcgcgagc agggccacgg cggcgtcggg
     4621 gccccaggtg cccgcctcgt agttcgggtg gtcgagcggc gggttctcgc gccaggcgtc
     4681 gagaagcggc gtgacgcgct cccacgccgc ctcgacctcg tcgcgccgcg cgaacagcgt
     4741 cgagtcgccg tgcatggcgt ccatgaggag gcgctcgtag gcctcgggct gcgacgcccc
     4801 gaagaactcc gagtaccgga agtccatcgt gacggggtca accttcatgg tcgggcccgg
     4861 ctccttcgcg ccgaacttca gcgcgatgcc ttcgtccggc tggatcctga ggatgagggc
     4921 gttcggctcc tgtctcacga cgccgacctg ccggaagagc gagatcggag gcgtcttgaa
     4981 ctggatggcg atctccgtga cgcgcttggc gaggcgcttg cccgcgcgca ggtagaacgg
     5041 cacgcccgcc caccgccagt tctcgacctg taccttccac gccgcgtacg tgtctcgcag
     5101 ggacatcggg ttgacgtcct tctcggagcg gtagccgacc accggctggc cgccgatcgc
     5161 gccggggccg tactggccgc gcaccgtgtc gcggatgatc tcctccggcg tctgcggctg
     5221 gatgcacttg aggaccttga ccttctcgtc gcgcaccgcg ttcgggtcga aggccacggg
     5281 cggctccatc gccacgaggc agaggagctg gagcatgtgg ttctgcacga tgtcgcgcag
     5341 gacgcccgac tcctcgtaga agcggccgcg gttctcgacc ccaatggact cggcgactgt
     5401 gatctggacg tgttcgacgt aacgccggtt ccagagcggc tcgaagatcc cgttgccgag
     5461 acggaagacg aggatgttct gcgtcgtctc cttgccgagg tagtggtcga tacggaagat
     5521 ctgccgctcg cggaagacgc gcgagagctc ctcgttgagg gcgcgcgcgg tcgcgagatc
     5581 gcgcccgaac ggcttctcga cgatgacgcg cgaccacggg ccgccgtcga cagggtagac
     5641 gaactgcgcg tccccgaggt tcttcacgac cgggccgatc atcgacggcg ggatcgcgag
     5701 gtaaaacatc cggttgcccg gcgtcccgcg ctccttttcg agcgcctcga ggcgcgtctt
     5761 gaggtccgcg aagctcttgc cgctgttcag gtcgccgcgg aagaagaaga gccgcgactc
     5821 gaaggtcctc cacttcgcct cgtccaccgg cttgacgcgc gcgaactcgg cgacggcctg
     5881 cttcatctcg gcgcggtacg tctcgtcgga ctcccccgtc cgggcgtagc ccacgatgca
     5941 ggtcccgggc gaaagctgtc cctgggcatc cagctcgaag agcgcgggga cgagctttct
     6001 cttcgaaagg tcgcccgagc ctccgaagat gacgatcgtc gcgggctctg gagttgcggc
     6061 gctcacggcg tcctcacctt ttcccggcga gggccgccct cacggccgcc gcgattccgg
     6121 ccccgtcgag gcccatgcgc cggtagagct ccttcgcggg gcccgaggag gcgtagcccg
     6181 tgacgccgag cttcacgacg cgcgccggga ggccgaggtc gttgaagcgc gcctgcagga
     6241 gtgtcccgag acccgtcttc gggttgtggt cctcgaccga gacgacgagg ccgtgccgcg
     6301 ccatgaacgc gaggtcctcg tccgcgaggt cgctccacgc gcagaccgag acgaggtcgg
     6361 cgcgcacgcc ttcctgcgcg agggcgttcc acgcgtcgag cgcgtgcggg agcatgttgc
     6421 ccgccgagac gagcgcgagc gggccgtcgc ccgaccggag aacgtccgcg cgcgacgggt
     6481 cgaacgccgc ctcccccgcg aagaaggccg tcccgtcctc cttgagcgcg aacgggatct
     6541 tcgagcggcc catgacgaga gcgtggttcc cgcggtgcgt cgccatccag cggacgacgc
     6601 ggtccgtctg gttcgggtcg gccggcgtga agaccttcca tccgaacgtc gagttcagga
     6661 ggccgaaggt gtcgatcgag tggtgcgtct tcccgtcctc gccgacgtcg atgcccgcgt
     6721 gcgtgacggc gagcttcgcg ttcgcgccgt tgatgtcgtt caggcgctgc tggttgtagg
     6781 tctccgcgat gccgaacatc gcgaactcgc cccaccacgc gtggacgccc gcgacggaga
     6841 gcgccccgag gaggaccgcg gcgttgtgct cggcgatccc gccctggaag aacgcggacg
     6901 gcaccttcgc ctcgaccgtc tccgtcttcg tcgagaccga gaggtcgcag tcgatcacgg
     6961 cgaacggcgt ccccgcggcg gccgccgtcg acgcgaggtc ggccagggcg ttcccgaacg
     7021 cgctccggtt gtccgtctgc ttgtcgagcg ggtaggtgcg cggcgtcccg ccggacgcga
     7081 ggacgacgga ctcctcgggc cggtgcggaa gggccttctt ccagtcgggc gcgggcttct
     7141 tccgctcctc gacccagcgg tcgagatcgt cctcgacgcc gaggatcgcg agcgcctcgc
     7201 ggcacttctc cggcgcgagc gccgcgccgt gccacttgaa gccgtccttc tccatgaagg
     7261 ggacgccctt ggacatcacc gtgtgcgccg cgacgagcgt cggcttctcc gcgcggaggg
     7321 ccgcccggag cgcgcggtag acggcgcgca cgtcgtgtcc gtccacctcg atcacgcccc
     7381 agccgtcggc ctcccagtcc gcgacgatgt cctgcctgag gatcgcgtcg ttcgacccgg
     7441 acagctgctc gcggttccag tccaggacgc cgacgaggtt cgtcagcccg tacttgaccg
     7501 cgaaccggcg cgcctcggcg agctggccct tctgctgctc gccgtcgccc atcgcgcaga
     7561 agaccttcga cccgtcgccc ttgatcttcg ccgccagcgc gaacccggca gccgccgaca
     7621 ggccctgccc gaggttgccc gtgccccact cgatccccgg cacgtcgcgc tcgacgtgcc
     7681 cctcgaacgg cgacccggcg cgccggaagc cgacgagcgg gagcgcccgg tcgaaccagc
     7741 cgaggtgggc gagcgccgcg tacgcggcgg gcgaggtgtg gccgtgactg atcacgatgc
     7801 ggtcgcgctt cggatcgaac gggttcttcg ggtcgcagtt cgcgaagccc cagagcacga
     7861 gctcgaagtc gatcgacgac atcgaacctc ccgggtggcc ggacgccgcg agcgtcgtca
     7921 tgcgcaggat gtcgccgcgg gccgcggccc cgagttctgc gagccgctcg acctgtgcat
     7981 cggcgagctc gggcgcttcg aaggtgccga gaatcaagcc ttcacccccg cggcggcctg
     8041 gcgccgctgc tcgatcgtcc cgaggagctg gtcgtaagac ttctcgaaga ggacgacgcc
     8101 gtccttctgc agctcctcgc caacggcctc gacgtcgatg ccgatcccgg cgagttcgga
     8161 gaggaccgtg cggctctccg cgacgccctc gagcacggtg tcggacgggg tcgcgctgcc
     8221 gtggtcgaga agcgcgtcga gcgtcttttc cggcatcgtg ttcacggtgt cggggccgac
     8281 gaggttctcg acgtagagaa gatccgagta ggccgggttc ttcgtgccgg tcgacgccca
     8341 gagcggccgc tgcagccgcg cgcccttccc ccggagcgac gtgaaccgct cggccccgaa
     8401 gatgtcccgg aagtcctggt agacgacctt gctgttcgcg aggccggccc ggccgcgcaa
     8461 ggactcgagg tgcgccttcc cggcgccgga ggccgccgcg gccttctcgt cgagacgcct
     8521 gtcgatcaga gtgtccacgc gggacacgaa gaacgacgcc acggagtgga cgcgggagag
     8581 gtcgccgccc ttcgccgcca ggtcctcgag gccaccgagg tacgcgagcg cgacgtcccg
     8641 gtagtgcctg cggttgaaca tgagcgtcac gttcaccgag atgccctccc cgatgagccg
     8701 gcggatcgcc gggacgccct ccggcgtcgc ggggaccttg atgaagacgt tggggcggcc
     8761 cagggccgcc gcgagccgct tcgcctcgcc gaccgtcttc tccgtgtcgc gcgcgagaga
     8821 gggcaggacc tcgagcgaga tccagccgtc cacgggattg cggtcgtgga tcgcgcggaa
     8881 cgcgtcggcg ccttcggcga tgtcctgctg cgtgagcgcg tcgtagatcg cgaaggcgtc
     8941 cttccccgcg cgcgcgaggc gctcgatccc cgcgttgtaa tcgccctcgt tcttctcgat
     9001 cgcctgcccg aagatcgtcg ggttcgaggt gaccccgtag atcgtcccgg cgtcgacgag
     9061 ggacttcagc cggccggtcc ggatcatctt gctcgagatg ctgtccagcc acacggcctg
     9121 gccgcggcgg cggtacgcga tcggtgcgct catggggtct ccctcacctc acggatcgta
     9181 ttacgacgtg gtctcgctct cgtggactcc gtagacgtgg aggcgcgccc cgagagccga
     9241 caggcgcgcg acgatcgcct cgcgcagctc gggaagcccc gcgccggtcg cggccgagat
     9301 ccggagtccg tcggcgtcga gcgcctcggg cgacgggcgg tccgtcttgt tgtaggcgag
     9361 gaggacgcgg cgcttctcga cgcccagctc ctcgaggacc tgctcggcga cggccttctg
     9421 ctcggacgcg gcgggtgagg acgcgtccac gacgtggacg aggaggtccg cgtcgcggac
     9481 ctcggcgagt gtggacttga aggacgccac gaggtcgtgc ggcagcttgc ggaggaaccc
     9541 gacggtgtcc gcgagcacga tcgcacgccc gccgacgccg tgcagacggg cgtgccgcgg
     9601 gtcgagcgtc gcgaacaggc ggtcctcggc gaattccttc gaggacgtga ggcggttgaa
     9661 gagcgtcgtc ttccccgcgt tcgtgtaccc gacgagggag accgtcgcga cgcgcttcat
     9721 gcccttgcgc cggacgtcgc gctgcgtctc gatcttcccg agctcttcct tcagcttcgc
     9781 gatgcgcatc cggaggcgcc ggcggtcgag ctcgatcttc cgctcgcctg ccccgccgcg
     9841 gaacgcgccg cccccgcgtt gctgggcgag gccctgggac ttgcccgcga ggcgcggcag
     9901 ctggtactgg aggctcgcga gctcgacctg tgtcatggcc tcgcgcgagc gggcgcgcgc
     9961 cgcgaagatg cggaggatga cgcccgcgcg gtcgaggacg ttcgtcggaa gcgtcttctc
    10021 gaggttgcgc gactgcgacg gcgtgagctc gtcgtcgaag atcgtccagc cggcgccctg
    10081 cgcccgcgcg acctcggcga tctcctcaag cttgccggag cccacgtacg tcgccgggtc
    10141 cggcgacctc cgctcctgca gggcgcgggc gacgacgacg ccgcccgccg tgtccacgag
    10201 gcgctcgagc tcgtcgaggt ggtcctccgc gagggcgcgc ggcacctcgc gcccgtagac
    10261 gcccacgagg attgtcttgt tcgagtcggg cggcggctgt gcctccgcgg gctcttcagc
    10321 gcctgatttc cgcattccgt tcgatcgtag cagccgccgg cctctgttag acttggccct
    10381 ccgccgtgaa gagcattctt cgaaacctgt gcctggctct gcttctcgcc ggcttcgtcc
    10441 tgacctacgc cggggggcct tccgtgccgc ccggtcacca ggcactcgcc cagggtccga
    10501 cgttgacgcc gacgagcacc cccaccacga cttcgacgcc cccggcgact tctacgccca
    10561 cgccgacgcc aacggcaacg ccgacctcgt tcggtaccac gacgcccacg gcaactgcca
    10621 cgccgacgtc caccgcaacg gcgactgcca cgtccaccgc aacggcgact gccacgtcca
    10681 ccgcaacgcc gacccccacg tccaccgcaa cgccgacccc cacgtccacc gcaacgccga
    10741 ttgccacgtc caccgcaacg ccgacgtcga cgcccacgcc ggtcaactcg gcgacgccga
    10801 ctccggccgc gacggccaca cccacgcccc tgccgagccc cacgttcacg ccgactccca
    10861 tcgggggcgg cggttcggcc gtcgacgttc cggccctcga cccgcgcgcg ctcggcgttc
    10921 tcgccgtggc cctcggtctc gccgggctcg ccttgacgag gttcctcttc ggaaagaact
    10981 gaacccggcg gcccgaggcg catgaaggtc ctgggacgtg aggcgcggcg gacgggggtg
    11041 ctcatcgccg ccgtcatcgt cgccgcggcc gcgtttcggc tcttccggct cgactgggac
    11101 gcgcgccagc acctgcatcc ggacgagcgg ttcctcacga tggccgagac cgcgctgtcc
    11161 tggccggaga ctctctccac gtacttcgac gaagcgcact cgacgctgaa cccgcgcaac
    11221 gtcggcttcc cgttctacgc ctacgggacc tttccgatcg tggcggtcaa ggcggtcgcc
    11281 tccgccctcc accggacgag cttcgacgag gtcaccctcg tcggccgtgc cgtatccgcc
    11341 gtggcggatc tcgccactct cgccctcctc ctcctgcttg cgtggcgcgt cagccgggac
    11401 cggcgcgtcg tactcctcgc cgggttcttc tacgccttcg ccgtcctgcc gattcagcag
    11461 gcgcacttct tcacagtcga ctccctcgca acctgtctcg cgaccgctgt gcttctctct
    11521 ctcgtcgcgg ttcagtccag cccgcggccc gcccgctttg cctgggccgg cgcgctcatc
    11581 ggtctcgcgc tcgcgaccaa gatcacgagc gccctcctcg tgctcgtggc cgccgtcgtc
    11641 gtggccgagg ccgtcctccg tgcctggaag cggggcggag cccctcgcgg tgcccccgtc
    11701 gagagccttc tgttccgttc caccctcgcg ggcatcgcgg ccttcagcgt cttccgcctc
    11761 gcgcagccag atgcgttccg gggtcccggc ctgctcggca tcctccccag cgaccgttgg
    11821 ctctcgaatt tcgccgaggc agcggcgctc gtgaacggct cacgcgacat tccacccggc
    11881 gtgcagtggg ccggccgcgt gatgctggcc tggccgctcg agaacctcgt cctttggggg
    11941 ttgggcgttc ctctggggct cgcggcgctt tgcggcacac tcgcggcggc ggcggctgtg
    12001 cagcggcgcc gcgagggtgc gctcctcgtt cccgtcgcgt gggtcgccgt cgtcctcgtg
    12061 tatcagggca cacagtgggt ggcttccatg aggtacttcc ttccggcgta ccctgctctc
    12121 tgcctcctcg ccgcatgggg cctcgtgagg cttcgcgatc gggccttgac caccgccgcg
    12181 cgacgcgcag ccgccggggc catcgtcctc acggggatcg gcacgcttct ctgggcgacc
    12241 gcgttcatgc gtatctacac gcggccccac ccgcgcatcg aggcgagccg ctggatctac
    12301 gcgaacgttt cgccgggcgc caccatcgcg tgcgaacact gggacgaccc gctcccgctg
    12361 aggctgagcg ggcccgaccc gttcccgtcg ctctacaccg ggttccagct gccgtggtac
    12421 gacgacgaca ctcccaaaaa gctcacgaag gtcctcgacg gactcgacca cgccgacgtg
    12481 ctcgttctct cgagcgatcg gctttccgga tccatcccgc ggatgcccct ccgctggccc
    12541 atgacgacgc gctactacga ggcgctcgag gacggctcgc tcggcttcga agaggccgcg
    12601 cgtttcacgt cgcggccggg cctgctcggc ctttcggtgc ccgacgagcg agcggaggag
    12661 gccttcaccg tctacgacca cccccgcgtc cgcatcttcc gcaagacccc ggcttactcc
    12721 cacgaccggg ccgcccggat tctcgacgtg gacctcggcc gcatcctgcg gatgactccc
    12781 cgcgaagcct cccgggcgcc gaacggactg atgctgagcg aggtcgcctg ggaacgcgtc
    12841 cgtcaccggg ggacatggtc ttccctcttc ggaccggcag cacccggcgc tttccggtcc
    12901 gtcatcctgt ggagtctcct tctgcttgtc ctcgggctcg tcggagcgcc tctcacggct
    12961 ctcgcgtgcc ggagtcttcc ggacggtgga ttcgcgttcg cccgcgccgt gggtctcctc
    13021 gcaacttcgt ggatcgcctg gctcgtcgcg agcctccgga tcgcgccgtt ctcgagcggt
    13081 acgctcatcg tcgcgctcgg catcctcggc gccgtgtcgg cagcggtcgc cgtagccagg
    13141 cggcgcgaac tcaaggctct cctcgcggag cgcggacggc tcctcgccgc tgaggaactt
    13201 ctcttctggt ccgctttcgt cgcgttcctg ctcatccgac gcgcgaatcc cgatctctgg
    13261 catcccaccc tcggcggcga gaagccgatg gacctcgcct tcctgacggc cgtcgtgaag
    13321 tcggaggatt tcccgccgta cgacccgtgg ttcgcaggcg gcgcgctcaa ctactactac
    13381 ttcgggttcg tgctcgcggg cgcgctcgtg aagttgaccg gcgtcgcacc cgccgtcgcc
    13441 tacaacctcg ccgtcccgac attcttcgcg ctcacggtcg cgggcgcctt cggcgccgcg
    13501 ctcgcgcttg ttgcaccggc gcgcggcgga gccgggcgtc gcacggccgg tttcgcgatt
    13561 ctcggcgctc tcttcgtcgc ggtgatggga aatctcggcg aggtgcgcct ttaccttgag
    13621 gccgcgcgat cggcgagccc ttcgacgtgg gccagcccgg tccccgggct caccgccctc
    13681 gtccggacgg cgaccggact cctggcgtcg gcgcgcgcgg ggacgccgct agtcgcgcat
    13741 ccggactggg cctactggac ggcaacccgt ctcatcggac acgcggaggc cgagcccggt
    13801 ccgatcaacg aaatgccgtt cttcacgtac ctcttcgggg acctccacgc ccacgcgctc
    13861 gcccttcccc tcacgcttct cgtcttcggg ctcgcggccg cgcacgccct cgcggaggcc
    13921 ggcgagtccc ggctcgcgga gggggcgcgc ctggccctgc ttgcgctcgg ccttggggcg
    13981 ctcgtggcca cgaacacgtg ggacgcgccg acctacggac tgctcgtctt cgtcgcgtgc
    14041 gtgatcggcg cattcgcccg aggcgactcc gccgcctcgc agctccgggg ggcattcatg
    14101 cgcgcgggcc tggtcgccgt gggcgccgcg gccctgttct ggcctttcct gcggtcctgg
    14161 ggccgccctt atggcggctt ctcggcctgg acgggaagct cgacttcgct cggtgcgttc
    14221 ctgctcatcc acgccatctt cctcttcgtg atcgtcctcg ccttcgccct cgagctgtca
    14281 cagcccggga atcgcgaggc ccttcggaac ttcgcgacac gggggtcgcc cgcgatcgcc
    14341 ttcgcgagaa acgcgaccgg ggtcgccgcg tttctcggcg gaattctcgt tctcctcggc
    14401 cggcccgtcg cgggagttct tgtcggcctc ctcgccctga ccgccgtcgt ctcgctctgc
    14461 tccggacacg acgcttcgtg gcgcctcacc ctcgccggcg cggcgacggg ttttggcgtc
    14521 cttctctcgg cggagctcat cgtcctgacg ggagacgtcg gacggatgaa caccgtcttc
    14581 aagttctccc tgcaggcctg ggtgcttctc gggctcgcgt ccgccgcgtg cgcggcccgc
    14641 atcgccgccg ttcttccggg gctcggcccc gccatccgcc atgcgttccg gtccgccttc
    14701 gccctcctcc ttttcggggg tcttctctat cctcttctcg cgacaccggc gcgcatccgg
    14761 gaccgcttcg acccggctgg ggcgcgcgga ctcgacgggt cttcgttcct gcggaacgcc
    14821 gtgatcgaca tgggcggggc ccggacgccg cttgactggg accggcaggc gttcgtgtgg
    14881 attcaggaga acgtctccgg cacgcccgtc gtcgccgagg cgaacacgga tccggttctc
    14941 tacggctggg gcaatcgcta cgcgatgttc acgggcctgc cgtcggtgat cggctggcgc
    15001 tggcacgagg tccagcagcg cgcggcgaca ggggccgagg ccatcgaccg gcggattgcg
    15061 gacctcgggc ggttctaccg gaccgaggac ctcgatgagg ccgcgcggct cgcccgccgg
    15121 tacaacgtcg aactcgtcgt ggtcggcccc ctggaacgtg cgatctatgg gaacaccggt
    15181 ctcgggaagt tcgcggccgg cgggcgattc accctcgtct acgagaaccc cggagtgcgt
    15241 ttgtaccgcg tgaatcctgg acctcgctcg tgacgggcct cgcttcgcga ctccgttact
    15301 acgcgtcatc cctcgtcacg ctggccgcgg gcgtccgcaa ccctctcgca gtgctcctcg
    15361 cgctcgccac gggtcgaaag ccgttcctac tcgaactcgc gaacgggctc cgctttcttg
    15421 tccgcggacc actcgacgtg tggatcgtga aggagacgtg cctcgacggt gagtacgacg
    15481 cctgggcgcc cgcccccggc gcgtgctccg ttgtcttcga cgtgggagcc ggcctcgggg
    15541 atttcgccgt gcacgcggcg cgccggtggc cagaggcgcg cgttttttcg ttcgaacccc
    15601 atcccgagac cttctctctc ctcgagcgga acctcgcgct cgacggcgtg acgcaggtcg
    15661 ttgccgtgcg cgccgcgttg acgggcgccg gaacggcgga ggcggtcctc cgcgaggggc
    15721 actcggcttc ggagcggtcc acagcgacgg gatcaggccc gggaatcgcc gtgccgtcga
    15781 ccacgctcgg ccgcgcgttc gagacgcacc ggatcgagcg ctgcggattt ctcaagatcg
    15841 acatcgaggg cgccgaattc gacgtccttc tttccgccga cgaggccacg cttgggcggg
    15901 tcgaggccct ctgcctggag tggcacgagg gcccgggccg gacgatcgag gccctcgcga
    15961 gccatctgag cgcgtgcggt ttcgccgtgc gcctgcggcg gagccccgtg cacgcccacc
    16021 tcgggctcct cgacgcccgg cgtgccgcgg agctcccgtg aggatcctct ccgtgctgac
    16081 gtactactcg ccccactgga cgggactcac gcgccacgcg gtccgtgtcg ccgaggacct
    16141 cgcgcggcgc gggcacgagg tgaccgtcct gtcgacccga cacacccccg acctccctcc
    16201 tcacgagacg cagggcggcg tgcgcatcgt ccgcgtcgag cccgtgtccc ggttctcacg
    16261 ggggatgttc gcgccgatgt tcccgctcgc ggcgctccgc ctgatggcgc gcacggacgt
    16321 cgttcagatt cacagcccgc tccccgaggc gccgctcgtg gccttcttcg ggcggctcct
    16381 cgacaagccg gtcgtcctca cgcaccacgc agacgtcgtg atgcccgccg ggctggcgaa
    16441 caggttcgtc gaacacgcgg ccctcgcggt cctcacgtcg gcggcccgcc tcgcgtcggc
    16501 ggtcacgtcc tacagccggg actacgcgga ccactcgccc tttctctcag cgttcgagga
    16561 ccgcctgacc tgcatcgcgc cgcccgtccg gcttcccgag ccggaccgca tcgcggcgcg
    16621 ggcctggcgg gattcgttcg cgccacacac acgcaacctc ttgggattcg ccggccgctg
    16681 ggtggaggaa aagggcttcg acgtcctgct tcgcgccttc cccctcgtcc tgcgggagct
    16741 gcccgacgct cacctcgtgt acgcgggcga gtccggagtc gtctacgaga actccttcga
    16801 ccggtgccgg cccctcctcg agccgctccg cgaccacgtc acctttctcg gcctcctgcg
    16861 cgacccggca ctcatggcga acttctacgc cctctgcgac ctcttcgtcc tcccgagccg
    16921 atccgacatg atggcgctcg tgcaggtcga ggccatgctc gccggaacgc ccgtcgtcgc
    16981 gtccgatatc cccggagccc ggatcgtggt cgaaaggacc ggattcggaa ggctcgcgcc
    17041 gcccgaagac cccgaaggac tcgcgcgcgt tctcgtcgag acactgcgcg atcgggaacg
    17101 cttccgcccg gagcccgggg ctgttgccgc cgttttcaac ccgcagacgt cgatcgacga
    17161 ctacgaggcg ctctttcacc gggttctggg ggacgcccga tgacgctgac acccggcgac
    17221 cgcgccgcgc tcgacaggct cctcgcccgg gagtgggact tcgccttcgc ccgccgcacc
    17281 cgtctcctgt tcgaggacct cgatccccgg ccgggagagc gcattctcga ctgcggctgc
    17341 gggatcggct ttcacctcct gctcctccag gcctggcgcg gcgtcgctcc atcgggaatc
    17401 gacatggacg tcgggcgcct ccggaaggcg aaccgcggag gcgcccggcc gagggtcgcg
    17461 ggagcggccc tgccgcgcct tcccttccgc gacgggacct tcgacaaggt cctggcctcg
    17521 gaagtgctcg agcacctgcc agacgaccgc gccgccctcg ccgagctctt ccgcgtgctg
    17581 aagccgggcg ggatcctcgc tctttcggtg ccgcacgccc gctttccctt ctggtgggat
    17641 ccgatcagcc gcgtctgggg cgcgctcggg ggaaacccga tccgccgagg gccgctcgtc
    17701 ggcatctgga cgggccacga gcggctgtac ctcccggaga acctggccgc acgcgcgtcc
    17761 gaggcgggat tccgcgtcga acgctgcgtc gaggccacga cgcacgcctt ccccttcagc
    17821 cacttcatcc tctacggcat cggcaagcct ctcctcgagt cgggccttct gccccgttcc
    17881 ttctcagagg gcgtcggccg cctctcgaca agcagcgccg cggacgtcgg atggtcctcc
    17941 tttctgcgtg gggctctcgc gcgcgtggac gctcgaaacg aggttgctcc ggcggccgcc
    18001 gaccggttcg tcaacatcgt gctccgggcc cgcaaaccgg agggaacctg agcctgccgg
    18061 ggcccggccg cttcgttcgc gccgccggcg ctgccgcgct cgccgtggcc ctctccgctg
    18121 cggcctggtt cggcaccgcc ggcaatcagt tctcgggccg gggcttcttc gcatgggccg
    18181 cgagtcttgt gatcctgtgg cgcctgctcg accggcccgg tccgccgcat ccgccggcat
    18241 tcccgatttc gcgtgcgacc ggggcaggcc tggtggtgat cctccttggg gcgaccgcgc
    18301 tgcggttcgc cgctctctcc tcctacccgc ccgacatgac gagcgaccat gtcgagaagc
    18361 tcctcgatgt ccagcggatc ctcgacggcg atcggccggt cttcatgggc aacaacggcg
    18421 gccgcgagag cctgcacttc tacggactcg ccctcctcgc gaaggcgacg ggccaaccgc
    18481 tggacttcga tcttctgaag gcaggttcgg gactcgtggg acttctcggc gtcctcgcgt
    18541 ctttctggct cggccggatc ctcgccggcg acgacggatc cgcgtttgca gacggcacgg
    18601 gactcctctg cgccgccctg gtctcggtga gctggtggca cgtgatgctg tcgcgcctcg
    18661 gactgcgcat cgtctggact cctttcttcg tgtgcctcct cgcgggcttt ctcgcccgtg
    18721 ctctccgtac ggggcggcgc cgcgcgtacc tcgccgcggg cctcacagtc gggttcgggc
    18781 tctacgccta ccaggcactc cggatggcgc ccgtcttcgt caccctcggc gggctcgcag
    18841 cgctcttcct ggcccgtccg gcgggcggga cgcgcgcgat ggccgccggg aacttcgccg
    18901 cgctggtcgc gctcgcggcc gccacggcag tcccgcttgc acgctatgcg cacgatgcac
    18961 cggcggtctt ctgggcgcgc acgaagggac gcatccttgg agacgacgcg gtctccgccg
    19021 ccgaccggca cgcggccctc cggtcgaacc tcagtcgcct cggatcgaac gcggcgcgtg
    19081 ccgcagggat gttgcacgta agcggcgacc gggcgtggat cacgggagcg ccccagggcc
    19141 cgccgcaggt ggatcggctt acgggcgttc tcctcgcggc tggcttcgca ggcctcgcgg
    19201 tgcgtctcgt gcggcggcgg gatcccggag acgccctcgt gcttcttgga ttgcccgtca
    19261 tgctgctgcc gacggcactc gccctcgcgc ggccgagcga agtacccagc gcgacccgag
    19321 cgagcggcgc cctgcccttc gcgctcttcc tcgcggcgtg ggccggggcg cacgccatcc
    19381 ggttgttcgc gcggcgggcg ggtgccgcgc ccgccgtgtc catcgtggct ctcggtctcg
    19441 ccgtcagcgc ccccgcgaac gcccacatct acttcggacc cgccatggac gcctaccgct
    19501 cctcgacgtt cccctacgcc gaagcaggcg cggtcctcgg cgcattcggt cgcgtccacg
    19561 gcgcaccggg caacgcgttc atggtgggcg ctccaaactg gtgggatcac cgggcactcg
    19621 cgttcacgtc gggcttcaac cgctgggaca acggcgtcct gcccgacccg ctcttcgggc
    19681 ggctcgacga gaaggtccgc accaacgccg gcacgcccct cgcggtgcgc ccggaggcgc
    19741 ctctcctctt cttcgtctca ccggacgaca ccggaacact cgaccgcctg cgaacgaagt
    19801 tcccggaagg ccgccttcgc cgcatccaca cctcgcggcc cggtcgggac tacctgctcc
    19861 tgtacgtgga ttcgcccctg ctacgatcgg gcccgtgagg ccccgatgac tcgcggcgcc
    19921 ggcccacgcg accttcccct cctcggcgag cgcctcgacg cgctgcgcgc ggactgggcg
    19981 gagaggaggc tggattcgga tcccctggag ttcgcaagga gagcgaagcc cgtggagcag
    20041 gaggtcacgg cctttctcgc ggcgtccctg gcgttcgggc gcgtcgcctc catccgcacg
    20101 tccattcgaa ggattctcga cgccctcgag gaccgccccg ccgctttcct cgagcgctgg
    20161 gacgagcgcc caatccccgc gctggagcat ttccggcacc gctgggtttc gggggaggac
    20221 gttgaagatt ttcttcgaat ggtgaagagg gcgaggcagg cgcacggctc tctcggcgcg
    20281 ctgttcgcgg cgggcgacga agaggaagag aagatcttct tagaaggtga agaggggctt
    20341 cccgccagcc gcgccgcgtt tccggactac acggcggctc tctcccactt tcatagaaat
    20401 cttctgtctt tctcttcttc ttccacacct tctcgcggcc tgaagtttct gctccccgag
    20461 cccagaagcg gatctgcctg caagcgttcg cacctcttcc tccgctggat ggtccggacg
    20521 gagggcttcg acctcggcct ctggaccggc gggagtttca cgcccgcgcg cctcctgctg
    20581 ccgatggaca cgcacgtcca tcgcatcgcg acctatctcg gcctcacgcg ccgcccgtcc
    20641 gccgaccttg cggcgtcgcg agaggcgacg ggctggctca ggcgcctcaa cccggaagac
    20701 cccgtcgcgt acgactgggc gctctcgcgc ctcggcatcc tcgccgagtg cgtcatcgag
    20761 cgcacgcgcc gccactgcga gcgatgcgcc gtgaggcccg tctgccgcgc gtccctcatt
    20821 ccttcccaag gagccgccgc cgcatgaacg actttctcga ccgcaggaag gccctcagcc
    20881 tcggcatcgc cgcgctcgtc tcggctgcca tcgcgttcgt cgccagggac gcgctccgcc
    20941 cgcccgcggt ccgtgctccc gcgcctgccg cgcccgagaa aacggccgtg ctgcccgccg
    21001 ggccgctcgt cgtgagagac cgccagggcg cggcccgcga cctcgccgtg aagtccggcc
    21061 ggggcctcat cctccacttc tgggccacgt ggtgcggccc ctgccgcgag gagatgcccg
    21121 ggctcgtgaa gttcgtcaag gagacgaaag gcgatccgaa cgtcgagttc ctcgccgtct
    21181 ccgtcgacga ggattggaag gtcgtcgacg cctggctcaa ggagcgcggc atcgcggacc
    21241 tcccgctcgc gctcgatccg aagggcgaca cggcgcgccg ggtcgggacg aagctgtttc
    21301 ccgagacctg gttcgtcgcg ccgtcgggca ggatcttcca ctacatcgac ggcccggccg
    21361 actggaacga ccccaggctc cgggcgctcg cggccgagtt cagccgcgcg tcagccgcga
    21421 ggtcctagtc ggccagggag agcgtgacgt ccgccctcgc gacgccgctc gcgggcacga
    21481 cgacggactg gaccttctcc ccgagcagct cgtgccacgc gtggagacgg tacgtgcccg
    21541 gcgcgacgcc cgggaacgcg aagcggccct cccggtccgt cacggcggca aacggcccgt
    21601 ccgcgacgaa gagccacgcg ttcatccagg catggacgtc gcacttgatg cgcacgacgc
    21661 cggccgtggt gaatctctcg gtgaagctct ggcccttgac cgggatcgcc ttgttgaaga
    21721 gcgcgtcgcc cttcacggtc gtcgcgtgga cgttgtggag gaccgggtcg gaattcagga
    21781 ccgtgacggg gacgccccgg ctcacgacct gcgcgtaggg ctcgaacgcg cagcccttct
    21841 ggtcgatccg gaacggacct ttcggaaggt caccgcccgt cgccccctcg agccacacga
    21901 aggcgtttcc gacctcccgg cccttgccga ggacgagcgc gggcgacggg cggtccgccc
    21961 tgccgcagat cgcaacgtcc gagatcacgg gccgcggcgc gagcttcggg acggggccgg
    22021 agagggtgac gacgccgaag acaccgtctc ttgcctccgg atgtggggca cctgaaacgg
    22081 cgctttcgga tttcttagaa ggtgaagagg gcgatgcgcc gctgcccgcc gcagcagcgc
    22141 cgccttcggc ccgcgctcct ccgcccctat taccttctaa gaagaaaacc accgctgcaa
    22201 cgagcagtga cgcttgcacg acgcgcacgg gtcttcgctc tcagtgcgac gcgacctgcg
    22261 tcggcgcctt cttgcgcggc gccgacggcg ccgtctggaa gtcgtttccg agcgaccaga
    22321 tgtggtcgcg cagcgccttc gtgacgcgct ccgggtcgcc gaggtaggcc ttcgcctccg
    22381 cctccgaccc ccagatcgcg acgaggtcct tcttcaggcc cgcgaacgcg ggcgtgtcga
    22441 gcgcgggggc gagcggcgag atgcgcttgt ccgacttgtc gtccaccttc gggaagttcg
    22501 tcggcatccg cgtgcccggc atccactcgt cgggccgctt gatccagtcc gcgatccagt
    22561 cgtagcgcag gcgcgtgtgc gccgccgcga ggatgggcgc gaggtcggcg ggcgtcttgc
    22621 cctcccggag ggcctgcgcg aacgcgtcct gcgacgtcgg gtggcactgg gcgcacttga
    22681 gcgacgcgaa cgtcgcggcg cccgccgccc tggagcgcgg gtccaaggcc tccacgcgcc
    22741 cgtcgaacgg gtaacgcgcc ttgtcctgcg ccgcgaaata ggccgtgagc gcgttgatct
    22801 gttcggtcgt gaagccgaac gtcggcatgc ggaccttgag ccacggccgg atctggcccg
    22861 tcttcggacc gtgcaggaac gcgaaaagcc attcgctctg caccttcgcg ccctcgcccc
    22921 ggatgatggg cggcgccatc gcgaggtcga tcttgctcgc ggtcatggct tcacggatcg
    22981 cgccgccctt gtcctcgagg acgtggcagc cctggcagtt gtagtccttc acgatgcgcc
    23041 gcccggcctc gatcgcgccg cgcggcccgg tcgcgcgggc ggtgagcgcg tcgctcatgt
    23101 cgtccttctg catcccgagg acggtggact ggacggcctc gatctcggcg ggcgagagcg
    23161 agaactgcgg catcttgagc ttctcctgga agctcttgac cttctcgcgg tcgtagctcc
    23221 ggggctggcc caccttctgg gcgagccagt cggcccgcgt gtgcgggacg ctcacgaacc
    23281 cgaagtcgag gaggtggacc ggcttgttcc cccattcgga gagctcgacg ccgatcggct
    23341 tggccttctc gaaaccggga atgttgtggc acgcgtagca gccgtagtgg gcgatgatct
    23401 tctcgcccac gaagaggtcc ttgtcgtgcg ccgacatctt cgcgaggtcc gccttcgcgt
    23461 cgacgaccga gcgggtctgc atgaggtact ccgagacgag ggcgtcgcgc tcggccggat
    23521 cggccttcgg cgcgtgcgag gccttgaact ccggctgcga cggccgcgac cagaggtacg
    23581 cgacgaggtc cgagatctcg tcatcggcca ggcgcaggtt cggcatccgc gtctcggggt
    23641 tgtacgcctt cgggtccttc agccacgcgg cgatccatcc gggcgtcgtc ttctcggaaa
    23701 ggccgatcag gttcgggccg tgctggcgcc agccgtccat cacgcgccgc tccgcgccgg
    23761 gctcggaaat gtggcagccc tggcatccga cgttctcgaa gaggaccttg ccccgcgccg
    23821 cgtcgccctt cgcgggggcg gggtacgcgg ccttgccgga cttctcgaac aggtacgccg
    23881 tcacgcccgc gaccatcgtg tccgtgagct tgtggccgga cggcgtgtcg aagttctcga
    23941 ggtagaagaa ccgcggcatc ttcgtgttgc tgcggaacgc gcgcgggttc acgagccagc
    24001 ggcccgtcca ctccggcgtc gtcttcgccg cgacgcgcgc gaggttcggc cccggcttcg
    24061 gaaggtcctc gatgcccctc atcttgtggc agctccagca gccgagcgcc tcgatcagct
    24121 tgcggccctg gtcgagcttc gcagccttcg ggtggcccgc gtcctgccgg tggcagcgga
    24181 ggcatcccgc ctcgacggcc ttcatcgggt acatcggaag gtcgttgaac ctgtcgacct
    24241 cccagccgta ggccttcatc cagcggccgg tctgcgtttc ctccatcagc tcgcggaggg
    24301 cgtcgtcgtc gacgggcatc cccttctcgt cccggcccgc atgccgctcc tcctcgagct
    24361 tctcgtaggc cgcgtagtcg ttcggcatgt ggcccgcgcg cgtgaaatcc gtcgcgcggt
    24421 cgcgtccgcc gtggcaggag gtgcagccga acgtcgcgta cgggtgtgga gagttcgagt
    24481 ccacgaacag gtcgaggcgc gggtgcgtcc tcagcacgga ctcggggtac ttctgcttgt
    24541 cctcgaagcc ccgcttctcg gccgcgatgt ggcacgtcac acagcggtcg acgcgctggc
    24601 tcctcaggaa gttcacgtcg ttcgagaggc cgtcgagctg gacctgctgg atcttgagcg
    24661 acggcgcgag catgtcggcg atcggcgcgt tgcgcacgaa gtagaacgcg tcgtcctgct
    24721 tgagcgtctt gagcttcgcc cgatagcggt cgtattcctc gtccagcttc ccgagcgcct
    24781 cctcggcgtc cttcttcgtc ttctggagtt ccgcgagcgc ggtcctcgtc ccgtcccacg
    24841 ccttctgcgc cgcctgctcg cggccgcggc tctcctcgta ggccgcccgg aggcgctcgt
    24901 aggcggcctt cgccttcgcc gtggccgccg acttcgggtc ttccttctgg cgcgtctcga
    24961 agacgtagcg cgccgtgtcc atctcggcct tcttgaatcg gaagtcctgg tccgcgtcgt
    25021 accaggcgcc ctcggccttc cggtccctct cctcggcctc ggcgaccgcc gcgcggttgc
    25081 ggcgcagctc acgcgcgccc tcgcgcttct ggtcgaggta cttctgctcg gcctccgggt
    25141 cgatcctctc ggcggccgcc cggagcgcct cgcgcgtctt cttccggtcg ttctcgacga
    25201 acttcttctg ccagctcttc cagtcgcgcc cgtagtcctg ccacgtcatc cacgcgaacg
    25261 ccgggagggc gaggatcgac acgatcgcga agactttcgt cagcttcgcg atgtcgtaat
    25321 ggtggtcgat tggttcgggc ggcggagctt tggccatttc agatgttgaa cgagaattcc
    25381 tggatgttga cgatgtactt gaggttgaag atccaccgca ggtacatctt gatcggcacg
    25441 gcgagcatga tgagaaacag gttcacgccc acgagatacc ggacggttcc catcttctcg
    25501 tagaagcggc ggaacagcgt cttcgagagc agcccgggaa gggcgcccat gtagaagagg
    25561 acgaggaaga tcccgggaaa ctcgcgcttg agccagaacg tcggaagcgc cgtgttcagc
    25621 catttcaccc agaagatctc ggagacgctc acgttgttca gcggctcgag cttgttcggg
    25681 tcccacgtct cgtacggacc gaagaagttc cagttcgggc cgcgcaggaa cgtcccgagg
    25741 atgatgagga cgacccacag gatgatgaac ccgaagcaga acatgaggat ttcccacttc
    25801 cgctcgcgga aggagaagta cccgttgccg cgcgggttcg tgtcgatgta cgggatcgcc
    25861 atgagtccca cgatgatgag cgtcgggagg acgacgcccg ccatccacgg gtcgaagtag
    25921 acgagcagct cctggaggcc gaggaagtac cacggcgcct tcgcggggtt cggcgccgcg
    25981 ccggggttcg cgggctcctc gagcggcgcc ttcaggacga tcgaccagac cgtcatgacg
    26041 atcgagcaga cgatcagggc gatgagctcg acgtagacga gatccggcca tgtgaagacc
    26101 ttcttgtcgc tgtcttcctt ctcgagcggc ggcaggccct tctcgaggcg ggcgtcgttc
    26161 aggtgcgcct ggtacatcgc gtaccagaag aagaacccga cgagaaagag cattccgagg
    26221 atcgggacgt tgtcgggcat gctcgcgacg atccggaagt tcggatccgt gaagctgaac
    26281 gcgaacccgc cgacgagcgt cgcgaagagg aggaggccgc cgcgcttcgt ccagagcggc
    26341 cggtagaaca tggccgagac gagtcccacc atcgacagcg tcaccgcgat ggccggagac
    26401 gcgagccagt tgatggcgtg cttgatcgcg ttcatcgcct acagcggccc cgagatcccg
    26461 ccgtctttcc tcacgcgcca gaagtggacc gccatgagga atgccgcgat gagcgggatg
    26521 aagacgcagt gcaggacgta gaagcgcagg agggcgttcg cgccgacgat ccggccgccg
    26581 aggaggccga agcgcgcgtc gctgcccgcc gtcacgaggt tgacgccgtt gaccgcgatc
    26641 tgggccgcga acggcccctc gtagcccgcg aggggcgtgg cgcgcgccat gttcgagccc
    26701 accgtgatcg cccagatcgc gagctggtcc cacggaagga ggtagcccgt gaaggagaga
    26761 agcagcgtga ggacgaggag gacgacgccc acgttccagt tgaactcccg aggcggcttg
    26821 taggagcccg tcatgaagac gcggaacatg tggatccaga ccgcgatgac catcgcgtgg
    26881 gcgccccacc ggtgcatttc gcgcatgacg ccgaacggca cctgttcgcg caggtcgagc
    26941 atgtcgccgt aggcgtactc gatcgtgggc cggtaataga acatgaggag aagacccgtg
    27001 aacgtcagga cgaggaagag aaagaacgtc atcccgcccg cgcaccacgt gtagcgcatc
    27061 ttcacgcccg acttccgggt cttgacgggg tggaggtgga ggacgacgtt cccgagcacc
    27121 gcgaggacgc gcttgcggtt cgtgttcggg atcccgacgc ggaagatgga cttccagatc
    27181 tgcgtctcga cgacggcccg ctggagcttg cgccaccgat gctcgagact cacgccgctt
    27241 ccctcttacc ttccaagaaa atcttcttca tcttctcttt ctatgtcgtc ttgatgaacg
    27301 cggcggggtc ggtccactgg cccttctcgt agaggaactt gcgggacttg tcgatgaaga
    27361 tctggccgtc gtccggcgag atcgagatcg cggcgcgctc gagcgggcgc ggcgtcgggc
    27421 cctcgaagtt gatgcccgac cggtagtagc ccgagccgtg gcacgggcac ttgaacttgt
    27481 tctgggcggc gagccagttc gggatgcacc cgaggtgcgt gcagaccgtg atgagcgcgt
    27541 agagcatctc cgtgttccgc acgatccaga cctggtactt ctccttccag cgctcgtcga
    27601 cctcgcccgc ctggtagtcc gaggggtagc cggccttgaa ggacgacggc ggctcgaaga
    27661 gaacgttcgg gaagagaaag cgcgccgtca acgtcaggag ggccccgacg ccggccgcga
    27721 acgcgagcca cgccaccgcc agccacgaca gaaacccgcg tcgcgacggc gacggagcct
    27781 cagtcgccgt ggtcgcggcc ggagcggttc ccgtggtgac cgtgtccgcc atgccctcct
    27841 ctttccggaa acgaccgtga aagtctcaaa aataacgggc cgcgtcaaga ttcgccgcgg
    27901 accgcgttac cgccgggggg actccaggat cttgcgggcc tcccgccgga ccctgagcga
    27961 cgggtccgcc tccaccatgc gcttccccag cgcctgcgcg tcggggtcct tgaggagcgc
    28021 gaggcctcgc agcccgttga ggagcgcatc ctcctgctgc tcggcggaga ggcccggcgt
    28081 ggcgcgcagc gtggcgcggt cgcacatccc gaggaggacg tccttgccgg acgcgtcgtc
    28141 gagcgtcgcg agcgcgaggg cggcgttcca gcgcacgtcc gtgacggggt cgccgagaag
    28201 ccgcttcagc gcgggcacgg cgtcgccgtc ctccatgcgc ccggccgcaa aggcggcggt
    28261 cttccgaacg ccggggtcct cggagtcgag gaggggccgc accttcgcgg ccgatttcgg
    28321 gtcgccgacg agcgcgagcg cccagagagc gtagaggcgg gtctcggcgt ccttgtcgtc
    28381 gagcgcgctc tcgaggagcg gaaccgactt cgggtcgccg aggcggccga ggacgagcgt
    28441 gagatagcgg cgcacccgcg ggtcgtcgga ccccgccccc tcgaacgcct tgatggccgc
    28501 ggccgagaac gcggcctttt cggccgggtc cgtcagggcg gggagcttct tcgtgagctc
    28561 gaacgcggcc tgccagcgct ggttgcggcc gcccgtgcgc acgtccgcga ggagctcgac
    28621 cggggtcttg tggtcgacgg cgagcgagcc gaacagccag aagacgaaga cggcgacgcc
    28681 cacgacgaac gcggggatca ggaagaactg gacgagcagc cgccccgcgg gggtggcgga
    28741 agagacggag tcggaggaag aagattcttc gaatggttca tggggctggc caggcggcgg
    28801 agtgcgttcc gtcatggcgc tgcaagccgc ctcgccgcgg ccctcccctc ttcaccttct
    28861 aggaaaatct tctcttctct tctttcttca gagcaccggc aggtactgct tcgcctgcgt
    28921 gatcgggttc ggcacgtaga tggcgtcctt gtcgcacacg gtctcgcaga ggcggcagga
    28981 gacgcacggg gccgcgtcca cctcggcgac cttgtagtgg aagttcggcg cgagcgggtc
    29041 cgccacgaga tggatgcagt cgaacgggca gatgtcgatg cagaactcgc aggccgtaca
    29101 caggtcgcgg tccacgatcg ccttcgggat gtctttcggc ttgaccttct ggacgatctc
    29161 gccgtgctgg tcctggatgc acgacacggg gcagtagacc gcgcagaccg agcagtcgat
    29221 gcagcggttc tggtggatca cgaacttctc gttcttgccg cccgtgatcg tgtgcgtcgg
    29281 gcacttgatc tcgcaaaccc cgcagccgat gcacgtgtcg tgaatgaagt gcggcatgtc
    29341 ccgtcccggg gacaccgtag catccggggc cgggcgctcg caagacgccg tctcaacggt
    29401 gcccggggcc ggggaggcgg aaaatggccc cggccccgaa ctttccggcc cgaaggctcg
    29461 ttacacccct caggaagaac ccatgaaaga gctccgatcc gccgtcaacg ccctgttcct
    29521 cttcgcgatc ttcctcctcg tctactcggc cgtcgtcccg ggacacgccc gcgggagcgt
    29581 ccgcggaccc cgcttcctca ggatgcaggt cgaggacaac caccagcccg ggcaccccgt
    29641 caaagtgtcc ctctccgtcc cttacggctt cgtttcgggc gcgctgcggt tcgcgtccct
    29701 cggcaaggtc cgccgggagc tggacctgca cttcgacgag tccgtggacg cttccgaggt
    29761 ccggacgatc gtccaggagc tgaaggacaa gcccgagggc accgacgtcg tccgcacgca
    29821 cgacgacgcg acgctcacgc tccgcaagga gggcggcctc gtcacgatgg aggtccggaa
    29881 gaaggaccgg gacaaggaga ccgtgacgct gaagctgccg tggcgcctcg tggacgcgct
    29941 cgcggagggc gacagggacc tcgacgtcaa cggcctcgtc tcccagctgc gcgacgcgcg
    30001 gcgcggcgac ctcctcgagg tcacggcccc cgacgcccac gtcaagatct gggtggagta
    30061 aggtccccta ctcgtcggcg aaggggtcgc ggcccatgtc cgcgaggagc ttgtcgagcc
    30121 ggtactggct cggcccgaac acgagccgca tctcgccgtt ctcgcagacg acgtccttga
    30181 cctgcacgga cagctccggc ggcaggacgc cggagaggtc cacgacgacg atgcggtcct
    30241 cggggtagaa gaacgcgcgg ccgggcggct gcatgtcgac gatcttcttg acgacccagt
    30301 tcgggatcgg gacgcgcttg aagacgcgca ggtcggcgat cgcgaacccg aggtacccgt
    30361 ccttgacgcg catgtcgcgg agctcgccgc ggaaggcgac cgggatcccc ttcttgacgc
    30421 gcaccgtgat ccccaggcgg ccgtcctccg ggtcgatcag gacctcgcgg acctcgtgca
    30481 cgttgcggat cagctggcga gcgaacgtgt tgagcgtcgt cgcgtcgatc gcgacgacga
    30541 gcccttccca gcggaccgtc agggcgggca ggtcgggcgt cgggggcagg ggcgcgggct
    30601 cgaacacgcc ctcatcgtac gccaattcct acagcacgta cgcgagcgcg aaccccacct
    30661 gcgcgcacat gagcatgagg tacatgtggg tccaggacgg gtggttctcg gtcgtcgcga
    30721 gctcgtccgg gttgcggctg agcagatgca ccgtgtacgc gccccacgcc gcgaggagca
    30781 ggccgagcgc cgtcaggagc cacgggttgc cggacaggag ccggtggccg ggcgcgaacg
    30841 ggtcgcccac ccacgcgagg cccgcgagga gcagccacgg caggacgaag aacggcgaga
    30901 tcatcgcgac cgcccggcgg ttcccgtact tcaccggcag cgtctcgcag ccgcccgcgc
    30961 ggtcgccctc ggcgtcggag aagtccttcg tcgaggaggc gccgaggaga aacaggccga
    31021 agaggccgcc gatgacccac ggctcggccg agaacgccga cgcgacgagc gtccagcccg
    31081 cgaccttcag gagcgtcccg cgcgggaccg cgatcgtcac gttcgcccag atcccgagcc
    31141 gcttcgtgcg cccgaacgcg ggcacggagt agacgagcgt ggcgaggagg ccgccgagaa
    31201 agaggaagaa gcactggtgc gtcggcaggg gcgcgagggc ccgcggcgca aacccggaaa
    31261 cggcgggcac gacgatccac cacacgggaa ccatcgcgag cgcgtacagg gtccacgtca
    31321 ggcgccaggc cgaagcgatc gacgcctcgc ccgtcacgag cggtcggtcc ggcttgttga
    31381 tccggtcgac gtcgaggtcc gtgatctggt tcaggacgtt ggacgcggcg ttcaggagcg
    31441 acgcgcagag cgccccaaga gccacgatcc cgaggagggc cggcgtcacg cggtgcgccg
    31501 gatcggggtt gtggacgctg ccgaaggcgc agacggcgcc cgagaggatg ccgaggagcg
    31561 gcgggagcag cgtgaacggc cgagtcaggc ggatcgccgt cttcagggtc acgagattcc
    31621 tccgaggagc gcggcgaggg atggcgcggc cgccgcgccg gcgagcgtgt tcacgaagtt
    31681 caggacgtgt ccgttcgaca cgcgccaccg ggcgcccgcg cgcccgagca ggctctcgag
    31741 gatcgtcccg agaagcgccg cgaggacgac cgcggccgcg ccggagggcg cgaggacgaa
    31801 cgcggccacg ccgacccagg ccagcagccc cgccgccg
//
LOCUS       DAOMFY010000015        30203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_487_length_30203_cov_8.479932, whole genome shotgun sequence.
ACCESSION   DAOMFY010000015 DAOMFY010000000
VERSION     DAOMFY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 30203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30203
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_487_length_30203_cov_8.479932"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1056)
                     /locus_tag="PLB01_02615"
     CDS             complement(22..1056)
                     /locus_tag="PLB01_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HQQ76222.1"
                     /translation="MTGLASLHPAYFAMVMATGIVSVACKLLGLAPVADALLAVNVAT
                     YAALWALTAARVARYPKRVFEDLVDHQRGVGFFTIVAATSVLGVQCLLVRDFGGAAAG
                     LFVLSIALWVVLTYGVFAAFTVKETKPTLADGINGGWLVAVVATESIANLGGLLARRL
                     SGPQDALHFFTLVFWLCGGMLYIWMISLIFYRYTFFHFSPSDLMPPYWINMGAMAISA
                     LAGTTLIANAAQSALLGRMLPFLLGFTALFWATATWWIPMLVILAVWRHGYRKLEMKY
                     DPLYWGAVFPLGMYAVSTLRLAQAFDVPFLLFLPRVFLFVALAAWAATFTGLLRHLAR
                     AAGRGASAAA"
     gene            complement(1053..2621)
                     /locus_tag="PLB01_02620"
     CDS             complement(1053..2621)
                     /locus_tag="PLB01_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ76223.1"
                     /translation="MSERRTARTVLAMNTLAFAVCFAVWMMNGVLVTYLVDSKVYAFD
                     KAAMGWLIGLPVLTGSLLRLPVGILTDRFGGRPVFAAVMLAAAAAAFLVSFADGFWGF
                     ALGGLGFGVAGSSFAVGVAYTSTWFPKEKQGTALGIFGMGNTGAAFTSMAAPFMLQAL
                     TSNGAELDRWRMLPRLYAAALLAMTALFWLFTTTRAGVRTSSVPLAQRLAPLKVVRVW
                     RFGLYYLLVFGGFVALAQWLIPYYVNVYSVSVSAAGVLAAAFSLPSGLFRAVGGWLSD
                     RYGARTVMYWVLGGCSACCLLLAVPRMDVFAPGEGVMAAGAGGVEAAGPERIVAAGTA
                     YPLRTRTERAFDDRDVMVWPSWSFWHEPAVKAGDTVVKKQLLARGVTHIFFQANRSVF
                     TGLALLLAAFMGIGMAAVFKYIPTYFPSDVGVVGGLVGVLGGLGGFFFPILFGYLLAA
                     SGIWTTCWIFFFGLSIACLVWMQRVVARMMAEGLPRLSREMEEPALAREVERLAQEME
                     GIAKRLRRQAPEAG"
     gene            complement(2618..3304)
                     /gene="narI"
                     /locus_tag="PLB01_02625"
     CDS             complement(2618..3304)
                     /gene="narI"
                     /locus_tag="PLB01_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HQQ76224.1"
                     /translation="MLNVLLFAAFPYVSIVLFLVVSIQRYRRDPFTFSSLSSQFLESK
                     ELFWGSVPFHLGILTLFFGHLVGFLFPRGMTLWNMVPVRLFVLESTALAAALLTLFGL
                     AGLILRRVSTSRLQVVTSPADVLVYVLLLFQIVTGLWVAIALRWGSAWYTQTVVPYLW
                     SLARFQPDIQRMVDLKLPAQMHVVGAFVLIAAFSFTRLVHALVAPVPYLWRPNQLVIW
                     NRFRGARRLS"
     gene            complement(3297..3899)
                     /gene="narJ"
                     /locus_tag="PLB01_02630"
     CDS             complement(3297..3899)
                     /gene="narJ"
                     /locus_tag="PLB01_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HQQ76225.1"
                     /translation="MRPAAAEAVDAARTLGHVAASLAYPGPSSALRALEAAAEMDPVE
                     PDMAAAFGRLANWLSRALPGAAEERYSGLFDLSPVCTLHLGYHLFGEGYQRGALLAGL
                     TAEMRRAGVPAEGDLPDFLPAVLRLLAALPPGEDRETLVDALLLPALARMTEALKDVD
                     SPWTEILRALPALLAPLGGGAPLPPPERALDADLEADAHA"
     gene            complement(3896..5389)
                     /gene="narH"
                     /locus_tag="PLB01_02635"
     CDS             complement(3896..5389)
                     /gene="narH"
                     /locus_tag="PLB01_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HQQ76226.1"
                     /translation="MDVRVQVSMVFHLDKCIGCHTCSIACKNIWTDRKGAEYMWWNNV
                     ETKPGTGYPTAWEDQEKYKGGWEKHGDKLEVRAVGKVRGAMTVFHNPNLPTIDDYYEP
                     FTYSYQDLFDAKEGADQPIAKPVSMITGKEMEIEAGPNWDDDLGGSPVYAAHDPNLAH
                     VSPEERAALFEVEKLVFFYLPRICNHCVNPTCVAACPSGALYKRGEDGIVLLSQERCR
                     AWRFCVTACPYKKTYFNWGTGKSEKCILCYPRLETGQAPACFHSCVGRIRYFGIVLYD
                     AEKIAPTLQAADEDLVAAQRRMILDPEDPEVIAGAKKNGIGDDVIASAQKSPVYKWVV
                     DWGLALPLHAEHRTLPMLFYVPPLLPVMAANGEQGYDTRIEGGFFGAIEKYRLPLTYM
                     ASLFGAGNEGVVRYALRKQLAVRNYRRAVTVGDIPLEEAKRLLAEADTSVEEAEAIYY
                     LTSLAPADDRFVIPPMHREEAIEMLDEPLAAKGFAGMGMRQKPERGS"
     gene            complement(5402..9016)
                     /locus_tag="PLB01_02640"
     CDS             complement(5402..9016)
                     /locus_tag="PLB01_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HQQ76227.1"
                     /translation="MKWIRDLVSPKTRSWEEFYRNRWQHDKVVRSTHGVNCTGGCSWN
                     IYVKEGIVTWEMQALDYPLLDRSLPPYEPRGCQRGISYSWYLYSPIRVKYPYVRGALL
                     DLWAEARKKHQDPVDAWASLQSDPEARSRYQRARGKGGFRRATLDQAMEIIAAANVHT
                     ARTYGPDRVVGFSPIPAMSQLSYAAGSRFLQLFGGVNLSFYDWYCDLPPASPETWGEQ
                     TDVAESADWFNSKMIAVMGSNPNMTRTPDCHFLPEARHNGTKLVVLSPDFSQVSKYAD
                     QWIPLHAGQDGAFWMAVTHVILKEFHADRETPAFQSYLKQYSDCPFLVELTPTVRGHR
                     AGRLVHAKDLAAYADEENGEWKYVVLDAKTGLPKMPGGAVGHRWGEADGKWNLKLEDA
                     KTGETIDPVLSLLGRKDEVLEVEFEQYEASNVVLRGVPSVTLETKGGKKVRVTTIFDL
                     LMARYGVGRGLQGEYPRDYDDAGLTYTPAWQEKLTGIDRNTVTSFAREWASTAEKTGG
                     KCSIIIGAGVNHWYHNNLMYRSGIAALILTGCVGKNGGGLNHYVGQEKLAPVAPWSYL
                     AFARDWASPPRLQNAPSWHYMHCDQWRYEAEFTEYQPVPDEPSATTLAKGHTADLQVK
                     AVKNGWLPFFPQWNESSLEVVKKARAAGAKTEAEVVRHTVERLKKGDLKFAVEDPDAP
                     ENWPRVWFIWRGNALMASAKGHEYFLKHYLGTHTNLIAKEKAEGKVHDVAWRDAPEGK
                     LDLVVDVNFRMDTSALYSDIVLPAATWYEKDDLNSTDMHSFIHPLSAAVPPAWESRSD
                     WDLFRAIAARTSELSVRHFPEPVEDLVMTSLQHDTNAEISQVEVKDWAKGECEAVPGR
                     SMAPMKIVTRDYRRLHQRFTALGPAIRDGIGAHGVKLDVTAAYDELVATRATETVEGA
                     TLPSIRDARQAADAILHLAPETNGEVAYDGYRYLEKRTGLKLTDLAEGARDSRTTYKD
                     LQAQPRRILNSPCWSGLVTDGRAYSAFTTQVERLVPWRTLTGRQHLYLDHPGYLDFGE
                     HLPTYKPVPRMAAYGDLRESVDDGLLLNYLTPHGKWHIHSTYSENHRMLTLSRGTEPL
                     WVSDRDAEKIGIVDNDWVEIHNDHGVVCTRAIVSARIPRGICIQYHAPERTIGVPKSP
                     LRGGRRAGGHNSLTRIRLKPVLMVGGYGQFTYHFNYWGPTGVNRDTAVRVRKLSRVEL
                     "
     gene            complement(9013..9870)
                     /locus_tag="PLB01_02645"
     CDS             complement(9013..9870)
                     /locus_tag="PLB01_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQQ76228.1"
                     /translation="MRRAVLRIFPLLLLSTPLAAQAPPAAAAQSPAEDLFARKCGSCH
                     TVGKGVRVGPDLKDVAKRRARAWLDTFVKTPSALLDSDADARNLLAEFKGVRMPDLGL
                     SDSDAKTLVDLVIRCSAEPCNLAGKFVPATNATERDMALGREVFLGLSPMKGGGAPCV
                     SCHTVRGAKSLLGGGRLAKDLTNVFARLGDEGLDNALKNPAFPLMNKLFADHPIDAGE
                     AFALRAYLYKANLGGEPARDDAWSVFLAGSLGAFGVLVALNAAWGRRLRGVRRPLTHP
                     REDGKEHRP"
     gene            complement(10042..11415)
                     /locus_tag="PLB01_02650"
     CDS             complement(10042..11415)
                     /locus_tag="PLB01_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ76229.1"
                     /translation="MSEPTGRILIVEDERAQRDALVQYLSGRGHTVAGVPTGEEALER
                     LARESFSLLVTDLRLPGIDGLAVVRRAVELDEEMGVLLITAFASVESAVEALRLGAHD
                     YLLKPLILEEVARKAANLLAQRELVRENARLRRMLQEQRPGTEIVAVSAAMQDVLGWV
                     RRAAASRSTVLVSGETGTGKEVVARAIHESSASRAEPFLAINLAAMPDTLVESELFGY
                     ERGAFTGAERRREGLLRAAGKGTVFLDEIAELSVSGQAKLLRALEAREVQPLGSDRTF
                     PFEARLVAATHRRLTDLVEKGAFREDLYYRLAVLQIHIPPLRERPEDVPGLVQKLLAR
                     HAERGGIPVPFVTADAMRALCRYPWRGNVRELANVLDRALILADDGRIDAEQIPADVR
                     NAGGPDLDLTAAIDRFERGYIAMALRLSQGNREKAAEDLGISPATLYRRLERLGLKGA
                     EPGRSRG"
     gene            complement(11412..12731)
                     /locus_tag="PLB01_02655"
     CDS             complement(11412..12731)
                     /locus_tag="PLB01_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ76230.1"
                     /translation="MSAARDLRLTAGAAAATVLLLFLAFEGIEQSFVRTEEARRAVHY
                     ARGISTSLLTAAVVGLLGWRQHRTRARVLREEAAARAAESRDARALLGFIVDATPAAL
                     VVLDPQYRIVRANRVAEKVHGAGLEGRRCFEALAACATRCDECPAAASFSSGAPETCM
                     RPHTDPRTGEVLAVESHPLDLPDGTRHVLLVERVVTEQRKLQARLVHQEKMAAFGLLA
                     AGIAHDLGNPLASIEAQMQLLGGTRLEPEPATIMTAVRMEVGRLGRILRELVDFARRR
                     RDEAMLVSVQSVTEDALRLLRHDPRTRDVRVTTDFDPEAPPVLMVEDHLMQVVLNLLM
                     NAFDAMPEGGALRIEVRPAGHGVALRVHDSGCGMDRTTLGRCFEPLFTTKAPGKGTGL
                     GLSISRDILHEAGGELELHSAPGRGTTAVVSLPAARVEAASAAGAMA"
     gene            complement(12728..13810)
                     /gene="moaA"
                     /locus_tag="PLB01_02660"
     CDS             complement(12728..13810)
                     /gene="moaA"
                     /locus_tag="PLB01_02660"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQQ76231.1"
                     /translation="MESPAAPLPAAAGCSRPGERRAERLGPAPDAGLTDPIGRRITYL
                     RLSILESCNLRCTYCRPAEEAGVAALPPRMETGEIVRLAALFVSLGVRKVRLTGGEPT
                     LHSGLVPVVRALAAFAPRPPLVALTTNGVLLPRLARPLAEAGLAQVNVSLDATTREAF
                     RAMTGRDRLDAVRAGIDAALASGISRVKINAVIVGGQNEDQLLPLALLARDLPVDVRF
                     IEYMPMPGSPREPGMTLPAEEIERRLARAFALEENPRETDSGPARTFSVAGFRGRIGL
                     ISPFSVRFCSDCNRLRVTARGALRLCLLGGGEADLLGPIRAGASDDELAVLVREALRG
                     KAERHPLERASEAAPPCATPMWAVGG"
     gene            13914..15962
                     /locus_tag="PLB01_02665"
     CDS             13914..15962
                     /locus_tag="PLB01_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HQQ76232.1"
                     /translation="MSSSATGRATRTSGWAGLLSRIAAGALVYLVVSGLPLRLFGFSP
                     VLEWTVLLHTLAGFLSPVPVGVYLWRHFLEYRADALTPSKVLGYCGLGAVLLAEATGL
                     WVTYLALFSDRVPPFARTLHLVPSVALLVLLFAHLWPLRKRTPAEGVAPETFSAARRG
                     YVAALLGAPAVALLLAFPASLVYDGPKTAGSFPKDYRKPYGQDRPFAPSLARTESNGP
                     LAPEALAGSASCGVAGCHPAIYAEWAVSAHRWAALDPAFQRVQEEMGKQNGPESTRYC
                     GGCHDPISLFSGAKVVFKEKLTEDLGFQEGVSCLSCHAVSKTDVQGNANYTVKPPKRY
                     LFENAKSAPLKLASYFLIRSYPRHHTDSLEKRLFKTPEYCAACHKQFVDQEVNNFGWV
                     QLQNQYDNWRKSKWNHPKDAKTTVECRECHMPLLDGPEPASGDTADYNRTATDGKHRS
                     HRFLGANQYIPTLLKLEGGEQQVDLVKKWLKGEVEIPEIANKWAKGAVVSLDLDVPAK
                     ARRGEPLKLRVVITSNKVGHDYPTGPLDLIQSWVEVEVKDETGRIVFASGKPDEKKMI
                     PPGTFLFKAEPVDRYGNLIDRHNLWEMVGVRYRRSLFPGFSDAAEYAFGCPGTPAGPS
                     PTRDVTVPAPPRGKLDVLATLHYRKLDQYLVDFLFPGKGLSAPITDLAQVRTTVEIVD
                     "
     gene            15964..18492
                     /locus_tag="PLB01_02670"
     CDS             15964..18492
                     /locus_tag="PLB01_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQQ76233.1"
                     /translation="MARPVLKRWTHRRVLAAAGVAALGACAILWFTKKDEDAPYTPGA
                     AVEGVTDVLKKDASGAGWGVVFKDVAQSRGIDFSHFSAGPRSTQLPEDMGSGCAFGDY
                     DGDGDWDLFVVDTIGPLTLTSGDVRASRGGCRLFRNDGGTFTDVTAEAGLSDLKGTYM
                     GAAWGDYDNDGFPDLVVTSYGGIRLFHNKGDGTFEEVTKVSGVGAFKGFYTGAVWGDV
                     DGDGLLDLYVCTYVDYRFDPKDAGKASKFGQAVSPFTINPSSYPPAPNLYFHNEGKGR
                     FREMALEAGIANAEGRSLSATFADLNDDGKPDLYVANDVSEGALFVNEGSGLFSDRGH
                     EAHVADYRGAMGIAVGDANGDGAPDLFVTHWIAEANALYLNKLGGGKSRTLDFEDAAE
                     RTGLGQVSTDDIAWGTAFLDVDGDGLPDLVVANGSTFEEPADTRKLVPMPMRLFRNLG
                     EKGFVDVAPLSGPDLTAPRVGRGLAIADVDGDLREEIAVVVNGGKLVLLKAEGGPPNH
                     RIAIRCEGRKSNRSAFGTKLVLEANGGRQVREIGAGSSYLSQSAPEAIFGLGAAAKAD
                     ALTVRWPSGATATFRDLAGDRAYLIVEGEASPRALPDNRAKTLAFWDAYNAARAAFAK
                     GDVAASMAAYRKALEIDPRHEDCRYSLANLLLEKGDRAAAKAELETLLRVQPKSQRGH
                     GALGDLLADPASGPLMDFAAARKHYESAGGIYSPETGWVVRTGEVALVTGDAAGAERA
                     FRKVLVSNPRSFQALYFLGYLASRQKKNDADGARLFDEAFAALGPQASPAPGEGDVKK
                     AEKALPARGAFAGHWGYLGQGAVKKDDAWRDLDGRISRGAPKRP"
     gene            complement(18511..19170)
                     /locus_tag="PLB01_02675"
     CDS             complement(18511..19170)
                     /locus_tag="PLB01_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQQ76234.1"
                     /translation="MEPPEISSRPKPLLRGVLHEVAAFVAAVAGAILFFRASGARARA
                     GALVYVFSLVTLLSVSAAYHRRNWPEKARAIWRRLDHSAIFVLIAGTYTPLSFLLGSR
                     LGWIFLGIVWGGALLGIVMSVAWVKAPKALVAVVCVLLGWAAVPLLPALKAALGTGAV
                     VLLAAGGVVYSLGAVVYAVKRPDPFPRIFGYHEIFHALVIAAAGCHFAVVARAVVALS
                     A"
     gene            complement(19229..19930)
                     /locus_tag="PLB01_02680"
     CDS             complement(19229..19930)
                     /locus_tag="PLB01_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQQ76235.1"
                     /translation="MAQTATRTVSTLDLAGADAPARAAALLARFDALAPGETLVLAEA
                     PALDLLEALRCERRGLFEWTPAGFAAGPAGIEVLRRDAASGTSRGVFEALAWDHDRLD
                     ALDGAAFSALAADDVAGARAVFAAFARGLGRHIRFEEEILFPTLEERAGFRPEAGPTA
                     VMRAEHVEIRACLAGIAAALDGGGAGAPAIRARMLSVLGGHNLKEEQILYPLADRALG
                     ASEADELVARIQRLV"
     gene            20038..20649
                     /locus_tag="PLB01_02685"
     CDS             20038..20649
                     /locus_tag="PLB01_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HQQ76236.1"
                     /translation="MILNAARSAALVAALAVLFPGRAAAHCDSVDGPVVTDARAALEK
                     GDVTPVLKWVAPEGEKEIRAAFGRAVAVRKLGPDARELADTSFYETLVRVHRAGEGAP
                     YTGLKPAGSVEPAIAKADAALAAGSADDFARLIAAHAADGLKTRFARVVEAKKHAGES
                     VAKGREYVAAYVDYVHWVEGVVTAVHRGPAAHEGHAAAAAHVH"
     gene            complement(20656..21771)
                     /locus_tag="PLB01_02690"
     CDS             complement(20656..21771)
                     /locus_tag="PLB01_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ76237.1"
                     /translation="MQRLLALVRKEFLQLRRDTITLRMIVMVPILQTMIFGYAINYDV
                     KHLKTVVLDEDRSWDSRELVAKMTASEYFDVVKYVGSEAALRHEIDAGHASVGLVIDA
                     DFGKDRRRGASAKALLVVNASDTTTASQAMAIAGGISNQMSVRTLAARAGWKESGPPV
                     DLRVRPWYNPDLRTATFIIPGLIAILLTFTLIQFTAMAIVRERERGTLEQLQVTPVTR
                     VEIILGKIAPFVVIGVFQLTLIIVLMRYLFRVPIAGSVLELYLVGLMFIAAVLGLGML
                     LSTLAKTQMQAMQMSFFFLLPFVFLSGYVFPIAGMPVFFRYVSYLIPAKYFIEVVRGI
                     VLRGAGLTELWEPVAWLTFYTVVIIGLAVSRFQKTTA"
     gene            complement(21782..23518)
                     /locus_tag="PLB01_02695"
     CDS             complement(21782..23518)
                     /locus_tag="PLB01_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ76238.1"
                     /translation="MIRAEKLVRSFGSRPALRGIDLSIARGEMFALIGPDGAGKTTFF
                     RIVAGLLQPTSGRAVRDDVPFGLVPQRFSLYEDLTVDENLALRARLYSVPSAEAAARA
                     RDLLARVGLDRFGSRLAGALSGGMKQKLALVSALLTRPALLLLDEPTTGVDPVSRREF
                     WQVLNALHHEGLTIVVSTPYMDEAEYATRLAFLDEGRVVATGTKEEILSKVPRDRGGA
                     EAVAPTLDDAFIFFSEEKEKEKDKDFLEGKEGERSSPSKKVFSSSSSSSEILLRCDDL
                     TRKFGAFTAVDRVSLAIPRGKVFGFLGPNGSGKSTTIRMLTGLLQPTSGTATAFGGLD
                     VTRDTEAWKKRLGYMSQKFSLYLDLTVRENLAFFGSIYGLSTARQSVRIRELAARLRF
                     EPILGELTENLSTGQRQRVALAAALLHEPELLFLDEPTGGVDPKGRRVFWDLIYELAA
                     ARGMTVLVTTHYMDEAEQCDGLAFILDGRIIASGSPAELKASLDGRLLEVPIASEPFA
                     ALASVQGDPSLEDAYLFGLRLRAVAHEGAADAAARLLSRYGTPAAAAPSLEDVFVSLA
                     RRETPQARTAAA"
     gene            complement(23515..24492)
                     /locus_tag="PLB01_02700"
     CDS             complement(23515..24492)
                     /locus_tag="PLB01_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ76239.1"
                     /translation="MPRSAVLAFAALAAACRNGGDPNVAVLSGRLEAPMVDLAPKVAG
                     RVVEVKVREGDRVKAGDLVARLDLGETALAPERDAKGLESAEARFNDLKVGSRAAEIA
                     QAEADVADKKAAVELARRELERQEVLLSKKVGTQRDTDTARTTMERAVANAKMASDRL
                     QLLREGFRQYQTEQARADVSRARTVLKQSESVAREAEIRAPADGVILHRMAEPGLLLG
                     AGQPAVTMAFLDRLYVRTYIPETQLGRVRVGSAAEVSVDAFKGRTFPGKVTEISPDAE
                     FTPKPVDTSRERVNMVFAAKVDLDAGWNAPLVPGQPADVRVKTAESAPK"
     gene            complement(24510..27659)
                     /locus_tag="PLB01_02705"
     CDS             complement(24510..27659)
                     /locus_tag="PLB01_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQQ76240.1"
                     /translation="MQALARLCVRRPVFASVLIIAFIVVGGVGYTRLGVDRYPKVDFP
                     TVAVITRLPGAAPEEVETEVTDKIEESINTIAGIDDLQSVSAEGISQVFVTFILEKNA
                     DVGAQEVRDKVNRVLAELPRDIDQPIVEKLDPDATPILSVALSGKRTLRETTEYADKV
                     LRRQIESVNGVGQVTILGGRSRRINVRLDTERLRAYGLSAVDVRRALQTQNVKIPGGT
                     LKAGSNEATVRVYGRVANAKEFEEIFITQRNGLPVRVGDVARVEDSEEDAERAASKDG
                     VPTVVLSVRKQSGTNTIAVIDALKGRLAEIAPQLPAGYSTEIVRDQSMFIKAAVDTVQ
                     EHLLVGGFLAALVVLLFLGNIRSTLIAAIAIPTSIVSTYALMYAMGFTLNVLTLLALT
                     LAVGIVIDDAIVVLENIFRFIEEKKVMPFQAAIDATKEIGLAVLATTLSLVAVFLPVA
                     FMGGIVGRFMNSFGLTMAFAILVSLLVSFSLTPMLSARFLKRDEKKAAAGHGASSKSR
                     GFYAAIDRTYTAMLKWSMTHRWAIVALCLVALLTVPLLFKVVPKNFLPYDDESQFEVN
                     FRAPEGTSLEATALIGNRISKDVKTLPGVEYALLTVGADDAKTPNVASIYVKLVDAGK
                     RKESQTTVMDRVRKEILPRYADAKLRTDVSYVNAFSSGQKNAEIMYVINGPDLDKLAK
                     VSEGLMEKLRKVPGIVDVDSTLIFGKPELGVTVGRSKASDLGVSVADVAETLRVLVGG
                     EKVSAFDDGGEQYDVYLRARSEDRADEASLKRFNVPSYRLGAVPLEDVASFRRGVGPS
                     EIRRLNRRRQVTIMANMNPGFSSQAALDTLVKETKALKLDASYTYRFVGRSKEQGRSA
                     QNFLLAFALSGIFMYLILAAQFESWLHPITILLALPLTVPFALLSILIFGQSVNIFTS
                     LGILVLFGVVKKNGILQVEFANQLRERGMERDAAVTLACRERLRPILMTTMAFVAGML
                     PLLVSSGPGSGTNRAIGSVIAGGQTLALLLTLLATPVAYSLFDDAQQAAGRLVRRMRK
                     QHAPAATPAAESRPAPAAS"
     gene            complement(27670..28719)
                     /locus_tag="PLB01_02710"
     CDS             complement(27670..28719)
                     /locus_tag="PLB01_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ76241.1"
                     /translation="MNPKSAGILLSGTLLLAACSRPNAASKASAAVPPIPAVTAPVEN
                     RVLPRILEVTGALAADESADVASERDGQVATVRVERGTYVEKGAVLATLDEREAKAQL
                     DQARANLAWTSSEVERYAELRRKQVVAKAEDQRKGTDLDLAKANLALAEKGFEDCTIR
                     APFSGVVTEKKISAGAFVRRGQAICGLVRIDPLRAELAIPEVAVPFIKVGQKGRLNVQ
                     SFPDRTFDAVVRYIGPSLRSEARTLVVEAVVPNSERILKPGLFVTARVELPKNEPTLL
                     VPSAAIVTDSGVSHVFVLGKDRVSERIVALGDRYGTSVEIRSGVAAGERVVVNPDRRL
                     VDGLDVVPGGSAAAR"
     gene            complement(28725..>30203)
                     /locus_tag="PLB01_02715"
     CDS             complement(28725..>30203)
                     /locus_tag="PLB01_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQQ76242.1"
                     /translation="NNDIALERESFRIADASILRADGSYDPTFQLDARYRNHTDPANS
                     LLSGAPYGEISPSSEGVSGAAGIGALLPTGGTIAVSAYGARDLTNNFLALLSPAYSTS
                     IGIDIRQPLLQNLSIDPARRAIRIASLEKERSTASLRSTVTDTVASVERAYWTLVAAR
                     RDVLVREASMRLAAEQREDTKSRIDVGTLPESDIAQPIAEYERRRGDLYASQENARRA
                     ELLLKLLLLNDESDPLWNQTLDPVDRPETPLVRPDLATALRVAEDKRPEIAEARARVA
                     QRNVEVDYTRDRLKPQLDVVAGWARRGLAGALNPNAPSNGFLGAPVAVPDALEGSFGR
                     SLGTITESRFPDEWIGLSLTVPVFNRSAKGDVAIAKAQKSQAEILLSQQRQRVAVEVR
                     NAVLTLETAAQRIDAAKAGRSAAETQLAAEKERYNVGLSTNFFVLTRQNELATAVLTE
                     IAALTDYRKALTDYSRTAGTLLDERGIEIAGDAPALRAAEGR"
BASE COUNT         4592 a        10197 c        11046 g         4368 t
ORIGIN      
        1 agtccgcgcg cgcgcgcggc gtcaggcggc cgcactggct ccccgccccg ccgcccgcgc
       61 gaggtgccgc aggaggccgg tgaacgtggc ggcccaggcc gcgagcgcca cgaagaggaa
      121 gacgcgcgga aggaagagga ggaacggcac gtcgaacgcc tgcgcgaggc ggagcgtgct
      181 gaccgcgtac atgccgagcg ggaagacggc cccccagtag agcgggtcgt acttcatctc
      241 gagcttccgg tagccgtggc gccagacggc gaggatcacg agcatcggga tccaccacgt
      301 ggcggtcgcc cagaagagag ccgtgaagcc gaggaggaac gggagcatcc gcccgaggag
      361 ggccgactgc gcggcgttcg cgatcagcgt cgtccccgcg agggcggaga tcgccatcgc
      421 gcccatgttg atccagtagg gcggcatcag gtccgacggc gagaagtgga agaacgtgta
      481 gcggtagaag atgagcgaga tcatccagat gtagagcatt ccgccgcaga gccagaagac
      541 gagcgtgaaa aaatggagcg cgtcctgcgg gccgctcagg cggcgggcga gaaggccccc
      601 gaggttcgcg atcgactccg tcgcgacgac cgccacgagc cagccgccgt tgatcccgtc
      661 ggcgagcgtc ggcttcgtct ccttcacggt gaacgccgcg aagaccccgt acgtgaggac
      721 gacccagagg gcgatcgaga gaacgaagag gcccgccgcc gcgccgccga agtcgcgcac
      781 gaggagacac tgaacgccga ggacgctcgt ggccgccacg atcgtgaaga agccgacgcc
      841 ccgctggtga tcgacgaggt cctcgaagac gcgcttcggg tagcgggcga cgcgcgccgc
      901 ggtgagcgcc cagagggcgg cgtacgtcgc gacgttgacg gcgaggagcg cgtccgcgac
      961 cggcgcgagg ccgaggagct tgcacgccac ggacacgatc ccggtcgcca tcaccatggc
     1021 gaagtacgcg ggatggaggc tcgcgaggcc ggtcacccgg cctccggggc ctggcgccgc
     1081 agccgcttcg cgatgccttc catctcctgc gcgaggcgct cgacctcgcg cgcgagcgcg
     1141 ggctcctcca tctcgcgcga gagccgcggc aggccttcgg ccatcatccg cgccacgaca
     1201 cgctgcatcc agacgagaca cgcgatcgag aggccgaaga agaagatcca gcacgtcgtc
     1261 cagatgccgg aggccgcgag gagatacccg aagaggatcg ggaagaagaa gccgccgagg
     1321 ccgccgagga cgcccacgag gccgcccacg acgccgacgt ccgaagggaa gtacgtcggg
     1381 atgtacttga acacggccgc catcccgatc cccatgaagg ccgcgaggag gagcgcgagg
     1441 cccgtgaaca cggagcggtt ggcctggaag aagatgtgcg tgacgccgcg cgcgaggagc
     1501 tgcttcttga cgaccgtgtc gcccgccttc acggccggct cgtgccagaa gctccacgac
     1561 ggccacacca tgacgtcccg gtcgtcgaac gcgcgctccg tccgcgtccg gagcggatac
     1621 gccgttccgg ccgcgacgat cctctcgggg cccgcggcct cgacgccgcc ggcgccggcc
     1681 gccatcaccc cttcgccggg cgcgaagacg tccatgcgcg gcaccgcgag gaggaggcag
     1741 caggcgctgc agccgcccag cacccagtac atgacggtcc gcgccccgta gcggtcggag
     1801 agccagccgc ccacggcgcg gaagaggccc gagggaaggc tgaaggcggc agcgaggacg
     1861 ccggccgccg agacggagac ggagtagacg ttcacgtagt aggggatgag ccactgggcg
     1921 agcgcgacga acccgccgaa gacgaggagg tagtacaggc cgaagcgcca gacccggacg
     1981 accttcaggg gggcgagccg ctgcgcgagc ggcacggagg aggtgcgcac gcccgcgcgc
     2041 gtcgtcgtga acagccagaa gagggccgtc atcgcgagga gcgcggccgc gtagagccgg
     2101 gggagcatgc gccagcggtc gagctcggcg ccgttcgagg tgagcgcctg gagcatgaac
     2161 ggggccgcca tgctcgtgaa cgcggcgccc gtgttcccca tgccgaagat gccgagcgcc
     2221 gtgccctgct tctccttcgg gaaccacgtc gaggtgtacg cgacgcccac cgcgaaggag
     2281 ctgcccgcga cgccgaaccc gaggcccccg agcgcgaaac cccagaagcc gtccgcgaag
     2341 ctcacgagaa aggccgcggc cgccgcggcg agcatcacgg cggcgaagac cggcctgccg
     2401 ccgaagcggt ccgtcaggat cccgacggga agtcggagga gggagcccgt caggacgggg
     2461 aggccgatca gccagcccat cgcggccttg tcgaaggcgt agaccttcga gtcgacgaga
     2521 tacgtcacga ggacgccgtt catcatccag acggcgaagc acaccgcgaa cgcgagcgtg
     2581 ttcatcgcga ggactgtccg cgccgtgcgg cgttcgctca tgagagccgg cgggccccgc
     2641 ggaatcgatt ccagatcacg agctggttcg ggcgccagag atacggcacg ggcgcgacga
     2701 gcgcgtggac gaggcgggtg aagctgaagg cggcgatgag gacaaacgcc ccgacgacgt
     2761 gcatctgcgc cgggagcttc aggtcgacca tccgctggat gtcgggctgg aaccgcgcga
     2821 gagaccagag gtaggggacg accgtctgcg tgtaccaggc gctgccccag cggagcgcga
     2881 tcgcgaccca gaggcccgtc acgatctgga agagaaggag gacgtacacg aggacgtcgg
     2941 ccggcgacgt gacgacctgg aggcgcgacg tggagacgcg ccgcaggatc agccccgcga
     3001 ggccgaatag cgtgaggagc gcggccgcga gcgccgtcga ctcgaggacg aagagccgca
     3061 cgggcaccat gttccaaagc gtcatcccgc gggggaagag gaagcccacg aggtgcccga
     3121 agaagagcgt gaggatcccg aggtggaacg gcacggagcc ccagaacagc tccttcgact
     3181 cgaggaactg cgaggagagg ctcgagaagg tgaacgggtc gcgccggtag cgctggatcg
     3241 agacgacgag gaagaggacg atcgacacgt acgggaacgc cgcgaagagt aggacgttaa
     3301 gcatgggcgt cggcctccag gtccgcgtcc agggcgcgct cgggcggagg aagcggagcc
     3361 ccgccgccga gcggcgcgag gagagcgggg agcgcgcgga ggatctccgt ccacgggctg
     3421 tcgacgtcct tcagggcctc cgtcatccgc gcgagcgcgg gcaggagaag cgcgtcgacg
     3481 agcgtctcgc ggtcctcccc gggcggcagc gcggcgagga ggcgcaggac ggccggcagg
     3541 aagtccggga gatcgccctc cgccgggact ccggccctcc gcatctcggc cgtgagcccc
     3601 gcgaggagcg cgccgcgctg gtagccctcg ccgaacaggt ggtagccgag gtgcagcgtg
     3661 cagaccggac tcaggtcgaa gaggccggag tagcgctcct cggcggcgcc cgggagcgcc
     3721 cgcgagagcc agttcgcgag ccgcccgaag gccgcggcca tgtccggctc gacagggtcc
     3781 atttccgccg ccgcttcgag cgcgcggagc gccgaggacg ggcccgggta cgcgagcgac
     3841 gccgcgacgt gcccgagcgt gcgggcggcg tcgaccgcct cggccgcggc gggcctcacg
     3901 agcccctctc cggcttctgg cgcatgccca ttccggcgaa gcccttcgcc gcgagcggct
     3961 cgtcgagcat ctcgatcgcc tcctcgcgat gcatcggcgg gatcacgaag cggtcgtccg
     4021 cgggcgcgag cgacgtcagg taatagatcg cctcggcctc ctcgacgctc gtgtcggcct
     4081 cggcgaggag ccgcttcgct tcctcgagcg ggatgtctcc gaccgtgacg gcgcggcggt
     4141 agttgcggac ggcgagctgc ttgcgcagcg cgtaccgcac gaccccctcg ttcccggccc
     4201 cgaagaggct cgccatgtac gtgagcggca ggcggtactt ctcgatcgcc ccgaagaagc
     4261 cgccctcgat cctcgtgtcg tagccctgct cgccatttgc cgccatcacg ggcaggagcg
     4321 gcggcacgta gaagagcatc gggagcgtgc ggtgctcggc gtggagcggc agcgcgagac
     4381 cccagtcgac gacccacttg tagaccgggg acttctgcgc ggaggcgatc acgtcgtcgc
     4441 cgatgccgtt cttctttgcg ccggcgatca cctcggggtc ctccgggtcg aggatcatcc
     4501 ggcgctgcgc cgcaacgagg tcctcgtcgg ctgcctggag ggtcggcgcg atcttctcgg
     4561 cgtcgtagag gacgatcccg aagtagcgga tccgtccgac gcacgagtgg aagcacgcgg
     4621 gcgcctgccc ggtctcgagg cgcgggtagc agaggatgca cttctcgctc ttgcccgtgc
     4681 cccagttgaa gtacgtcttc ttgtaggggc aggccgtgac gcagaagcgc cacgcgcggc
     4741 accgctcctg gctcaggagg acgatcccgt cctcgccccg cttgtagagc gcgcccgacg
     4801 ggcaggccgc gacgcacgtc gggttgacgc agtggttgca gatccgcggg aggtagaaga
     4861 agacgagctt ctcgacctcg aagagggccg cgcgctcctc gggcgagacg tgcgcgaggt
     4921 tcgggtcgtg cgccgcgtag acgggcgagc cgccgaggtc gtcgtcccag ttcgggccgg
     4981 cctcgatctc catctccttg cccgtgatca tcgagaccgg cttcgcgatc ggctggtccg
     5041 cgccttcctt ggcgtcgaag aggtcctggt agctgtacgt gaacggctcg tagtagtcgt
     5101 cgatcgtcgg gaggttcggg ttgtggaaga ccgtcatcgc gccccgcacc ttcccgacgg
     5161 cgcggacctc gagcttgtcc ccgtgcttct cccagccgcc cttgtacttc tcctggtcct
     5221 cccacgccgt cggatagccg gtccccggct tcgtctcgac gttgttccac cacatgtact
     5281 cggcgccctt gcggtccgtc cagatgttct tgcaggcgat cgagcaggtg tggcagccga
     5341 tgcacttgtc gaggtggaag accatggaga cctgtacgcg cacgtccatc gtcttctctc
     5401 cttacagctc gacccgagac agcttgcgga cccgcacggc ggtgtcccgg ttcacgccgg
     5461 tgggacccca gtagttgaag tggtacgtga actgcccgta cccgccgacc atgaggacgg
     5521 gcttcagccg gatccgcgtc aggctgttgt ggccgccggc gcggcgcccg ccgcggagag
     5581 gcgacttcgg cacgccgatc gtccgctcgg gcgcgtggta ctggatgcag atcccgcggg
     5641 ggatgcgggc cgacacgatc gcgcgcgtgc agacgacgcc gtggtcgttg tggatctcga
     5701 cccagtcgtt gtcgacgatc ccgatcttct cggcgtcccg gtcgctgacc cagagcggct
     5761 cggtcccgcg cgacagcgtc agcatccggt ggttctccga gtaggtcgag tggatgtgcc
     5821 acttcccgtg gggcgtcagg tagttgagaa ggaggccgtc gtcgacgctc tcgcggaggt
     5881 cgccgtacgc cgccatgcgc gggaccggct tgtaggtcgg caggtgctcg ccgaagtcga
     5941 ggtagccggg gtggtcgagg tagaggtgct ggcgccccgt gagcgtccgc cacgggacga
     6001 gacgctcgac ctgcgtcgtg aaggccgagt acgcgcggcc gtccgtgacg agccccgacc
     6061 agcacgggct gttcaggatc cgccgcggct gcgcctggag atccttgtac gtcgtccgcg
     6121 agtcccgcgc cccctcggcg aggtccgtga gcttcagccc cgtccgcttc tcgaggtacc
     6181 ggtacccgtc gtacgcgacc tcgccgttcg tctcgggcgc gaggtggagg atcgcgtccg
     6241 ccgcctgccg ggcgtcgcga atggacggca gggtcgctcc ctccaccgtc tcggtggccc
     6301 gcgtcgccac gagctcgtcg tacgccgccg tgacgtcgag cttgacgccg tgggcgccga
     6361 tcccgtcgcg gatcgccggc ccgagcgccg tgaagcgctg gtggaggcgc cggtagtcgc
     6421 gcgtcacgat cttcatcggc gccatggagc ggcccggcac cgcctcgcac tcgcccttcg
     6481 cccagtcctt gacctcgacc tgcgagatct cggcgttcgt gtcgtgctgg agcgacgtca
     6541 tgacgaggtc ctcgacgggc tccgggaagt gccggaccga cagctcggac gtgcgggccg
     6601 cgatcgcgcg gaagaggtcc cagtccgagc gcgactccca cgccggcggg accgccgccg
     6661 agagcggatg gatgaacgag tgcatgtccg tggagttgag gtcgtccttc tcgtaccagg
     6721 tcgcggccgg caggacgatg tcggagtaga gcgccgaggt gtccatccgg aagttgacgt
     6781 ccacgacgag gtcgagcttt ccctcgggcg cgtcgcgcca cgcgacgtcg tgcaccttgc
     6841 cctcggcctt ctccttcgcg atcaggttcg tgtgcgtgcc gaggtaatgc ttcaggaagt
     6901 actcgtgacc cttcgcgctc gccatgagcg cgttgccccg ccagatgaac cagacgcgcg
     6961 gccagttctc gggcgcgtcc gggtcctcga cggcgaactt gagatcgccc ttcttgagcc
     7021 tttcgacggt gtggcggacg acctcggctt ccgtcttcgc ccccgcggcc cgcgccttct
     7081 tcacgacctc cagcgagctc tcgttccact gcgggaagaa ggggagccag ccgttcttga
     7141 cggccttgac ctggaggtcc gccgtgtggc ctttcgcgag cgtcgtcgcc gacggctcgt
     7201 cgggaaccgg ctggtactcc gtgaactccg cctcgtagcg ccactggtcg cagtgcatgt
     7261 agtgccacga cggggcgttc tggaggcgcg gcgggctcgc ccagtcgcgc gcgaaggcga
     7321 ggtacgacca cggggcgacg ggggcgagct tctcctgccc gacgtaatgg ttgagcccgc
     7381 cgccgttctt cccgacgcac ccggtcagga tcagcgccgc gatcccggag cgatacatca
     7441 ggttgttgtg gtaccagtgg ttgacgccgg cgccgatgat gatcgagcac ttcccgcccg
     7501 tcttctcggc ggtgctcgcc cactcccgcg cgaagctcgt gacggtgttg cggtcgatcc
     7561 cggtcagctt ctcctgccac gcgggcgtgt acgtgagccc ggcgtcgtcg tagtcccgcg
     7621 ggtactcgcc ctggaggccg cggccgacgc cgtaccgggc catcaggagg tcgaagatcg
     7681 tcgtgacgcg gaccttcttc ccgcccttcg tctcgagcgt gacggacggg accccgcgca
     7741 ggacgacgtt cgaggcctcg tactgctcga actccacctc gaggacctcg tccttgcggc
     7801 cgaggaggga gaggaccggg tcgatggtct cgcccgtttt cgcgtcctcg agcttcaggt
     7861 tccatttccc gtcggcctcg ccccagcggt ggccgacggc cccgcccggc atcttcggga
     7921 ggcccgtctt cgcgtcgagg acgacgtact tccactcacc gttctcctcg tcggcgtagg
     7981 cggcgaggtc cttggcgtgg acgagccgcc ccgcgcggtg gccgcggacc gtgggcgtca
     8041 gctcgacgag gaacgggcag tcgctgtact gcttgaggta agactggaac gcgggcgtct
     8101 cgcggtcggc gtggaactcc ttcaggatga cgtgcgtgac ggccatccag aacgcgccgt
     8161 cctgccccgc gtggagcggg atccactggt ccgcgtactt cgagacctgt gagaagtccg
     8221 gcgacaggac gacgagcttc gtcccgttgt gccgcgcctc ggggaggaaa tggcagtccg
     8281 gggtccgcgt catgttcggg ttggacccca tcaccgcgat catcttggag ttgaaccagt
     8341 cggcgctctc ggcgacgtcc gtctgctcgc cccacgtctc gggcgacgcg ggcgggaggt
     8401 cgcagtacca gtcgtagaag gagaggttca cgcctccgaa gagctggagg aaccgcgagc
     8461 cggccgcgta ggagagctgg gacatcgccg ggatcgggga gaagccgacg acgcggtcgg
     8521 gcccgtaggt ccgggccgtg tggacgttgg cggccgcgat gatctccatc gcctggtcga
     8581 gcgtggcccg ccggaagccg cccttgccgc gcgcgcgctg gtagcggctc cgcgcctcgg
     8641 ggtccgactg gagcgacgcc cacgcgtcga ccgggtcctg gtgcttcttc cgcgcctcgg
     8701 cccacaggtc cagaagcgcg ccgcggacgt acgggtactt cacgcggatg ggggagtaga
     8761 ggtaccagga gtacgagatc ccgcgctggc agccgcgcgg ctcgtacggg gggagcgagc
     8821 ggtcgaggag cgggtaatcg agcgcctgca tctcccaggt gacgatccct tccttgacgt
     8881 agatgttcca ggagcagccg cccgtgcagt tcacgccgtg cgtcgagcgg acgaccttgt
     8941 cgtgctgcca gcggttccgg tagaactcct cccacgaccg ggtcttcggg ctgacgaggt
     9001 ccctgatcca cttcacggcc gatgttcctt tccgtcctcg cgggggtgcg tgagcggccg
     9061 cctcacgccg cgcagccggc ggccccaggc cgcgttgagc gcgacgagga cgccgaacgc
     9121 cccgaggctg ccggcgagga agacgctcca tgcgtcgtcc cgcgccggct cgccgccgag
     9181 gttcgccttg tagaggtacg cccggagcgc gaacgcctcg ccggcgtcga tcgggtggtc
     9241 ggcgaagagt ttgttcatga gcgggaacgc ggggttcttg agggcgttgt cgagcccctc
     9301 atcgccgagc cgcgcgaaga cgttcgtgag gtccttcgcc aggcgcccgc cgccgagcag
     9361 gctcttcgcc ccccgcaccg tgtggcacga gacgcacggc gcgccgcccc ccttcatcgg
     9421 ggaaaggccg aggaagacct cgcggccgag ggccatgtcg cgctccgtcg cgttcgtggc
     9481 ggggacgaat ttccccgcga ggttgcaggg ctccgccgag cagcggatca cgaggtcgac
     9541 gagcgtcttc gcgtcgctgt cggagaggcc gaggtcgggc atcctcacgc ccttgaactc
     9601 ggcgaggagg tttcgtgcgt ccgcgtcgct gtcgaggagg gcgctcggcg tcttcacgaa
     9661 cgtgtcgagc catgcgcggg cgcgccgctt cgcgacgtcc ttgaggtcgg gcccgacgcg
     9721 cactcccttt ccgaccgtgt ggcaggagcc gcacttgcgc gcgaagagat cctcggccgg
     9781 ggactgcgcg gccgcggcgg gtggagcctg cgcggcgaga ggcgtcgaga ggaggaggag
     9841 aggaaagatc cggaggaccg ctcgtctcac gctgttccct tcctcgcgga ccgtcgtttt
     9901 cgccgggccg aggcctcgag gagcgcaagc ggcgtaccag ggccgaagac cccgcgggac
     9961 gcgctccgcg gccgcgggcg ctctcatttc tgagagaacc gggcccggga gccgctcagg
    10021 tttgagagtc gcggagcccg ttcagccgcg ggaacggccc ggctcggcgc ccttgaggcc
    10081 gagccgctcg aggcgccggt acagcgtcgc cggcgagatg ccgaggtctt cggcggcctt
    10141 ctcgcggttt ccctgcgaca ggcgcagcgc catcgcgatg tagccgcgct cgaagcggtc
    10201 gatcgccgcg gtgaggtcga ggtcgggtcc gcccgcgttg cgcacgtccg cggggatctg
    10261 ctcggcgtcg atccggccgt cgtccgcgag gatgagcgcg cgatccagca cgttcgcgag
    10321 ctcgcgcacg tttccccgcc acgggtaccg gcagagcgcg cgcatcgcgt cggcagtgac
    10381 gaaggggacg ggaatgccgc ccctctccgc atgccgcgcg aggagcttct ggacgaggcc
    10441 ggggacgtcc tcgggccgct ccctcagggg cgggatgtgg atctggagaa ccgcgagccg
    10501 gtagtagagg tcctcgcgga acgctccttt ttccacgaga tcggtgaggc gccggtgggt
    10561 cgccgcgacg aggcgggcct cgaaggggaa cgtgcgatcg ctcccgagcg gctggacttc
    10621 ccgcgcctcg agggcacgca gcagcttggc ctgaccggaa accgagagct ccgcgatctc
    10681 gtcgaggaag acggttccct ttcccgccgc gcgcagaagc ccctcgcgcc ggcgctcggc
    10741 gcccgtgaac gccccgcgct cgtatccgaa gagctcgctc tcgacgagcg tgtcgggcat
    10801 cgccgcgagg ttgatcgcga ggaacggctc cgcgcgcgac gcgctcgact cgtgaatggc
    10861 ccgcgcgacg acctccttgc ccgttccggt ctccccgctc acgaggaccg tcgaccgcga
    10921 ggccgcggcc ctacgcaccc agccgaggac gtcctgcatc gccgccgaaa cggcgacgat
    10981 ctcggtcccg gggcgctgct cctggagcat ccgccggaga cgcgcgttct cccgcacgag
    11041 ctcgcgctgc gcgaggaggt tcgcagcctt ccttgcgact tcctcgagga tgaggggctt
    11101 caggaggtag tcgtgcgccc cgagccgcag cgcctcgacg gccgattcga ccgacgcgaa
    11161 ggcggtgatc aggaggacgc ccatctcctc gtcgagctcg acggcgcgtc tcacgacggc
    11221 gaggccgtcg atccccggga ggcgcaggtc cgtgacgaga agggaaaagc tctcccgcgc
    11281 gagacgttcg agggcctcct cgcccgtcgg gacgccggcg acggtgtggc cgcgtccgga
    11341 gaggtactgc acgagggcgt cgcgctgggc gcgctcgtcc tcgacgatca ggatgcggcc
    11401 cgtcggctcg ctcatgccat ggctcctgcc gcgctcgccg cctcgacgcg cgcggccggc
    11461 agcgagacga cggccgtcgt gccgcggccg ggcgcgctgt gcagctcgag ctcgcccccg
    11521 gcttcgtgga ggatgtcgcg cgagatgctg agcccgagac ccgttccctt gcccggcgcc
    11581 ttcgtcgtga agagcggctc gaagcagcgc ccgagggtcg tccggtccat tccgcaccca
    11641 ctgtcgtgta cccgcagggc cacgccgtgc cccgcgggcc gcacctcgat gcggagcgct
    11701 ccgccctcgg gcatcgcgtc gaaggcgttc atgaggaggt tcaggaccac ctgcatgagg
    11761 tgatcttcca ccatgagcac gggaggggcc tcggggtcga agtcggtcgt gacgcggacg
    11821 tcccgcgtgc gcgggtcgtg ccggagaagg cgcagggcgt cctcggtcac ggactggacc
    11881 gagacgagca tcgcctcgtc gcggcggcgg cgggcgaagt ccacgagctc gcggaggatc
    11941 cgcccgagcc ggcccacttc catccgtacc gcggtcatga tcgtcgcggg ctccggctcg
    12001 aggcgcgtgc cgccgaggag ctgcatctgc gcttcgatcg aggcgagcgg gttgccgagg
    12061 tcgtgcgcga tgccggcggc caggaggccg aatgccgcca tcttctcctg gtggacgagg
    12121 cgagcctgga gcttccgctg ctcggtgacg acgcgctcga cgaggaggac gtggcgcgtc
    12181 ccgtcgggga ggtcgagcgg gtggctctcc acggcgagga cctcgcccgt ccgcgggtcg
    12241 gtgtgggggc gcatgcacgt ctccggcgct ccggacgaga agctcgcggc cgccggacat
    12301 tcgtcgcacc gggtcgcgca ggcggcgagg gcctcgaagc agcgtcgccc ctcgaggccg
    12361 gcgccgtgga ccttctccgc gacacggttg gcgcgcacga tgcggtactg cgggtcgagg
    12421 acgacgagcg cggcgggcgt cgcgtccacg atgaagccga ggagcgcccg cgcgtcgcgg
    12481 gactcggcgg cgcgcgccgc ggcctcctcg cggaggacgc gggctctcgt gcggtgctgg
    12541 cgccagccga ggaggccgac gacggccgcc gtgaggaggc tcgtcgagat gccgcgggcg
    12601 tagtgcacgg cccgccgcgc ctcctccgtc cggacgaagg attgctcgat cccctcgaac
    12661 gcgaggaaga ggaggaggac cgtcgcggcc gccgcccccg cggtgagccg gaggtcgcgg
    12721 gccgcgctca tccgccgacc gcccacatgg gggtcgcgca cgggggagcc gcctcgctcg
    12781 ccctctcgag aggatggcgc tcggccttcc cgcgcaaggc ctcgcggacg agcacggcga
    12841 gctcgtcgtc ggaggcgccc gcgcggatcg gtccgaggag gtccgcttcg cccccgccga
    12901 gaaggcagag ccggagcgct ccccgcgccg tcacacgcag ccggttgcag tcgctgcaga
    12961 agcgcaccga gaacggggag atgaggccga tccggccgcg gaagccggcg acggaaaacg
    13021 tgcgcgcggg gcccgagtcg gtttctctcg ggttctcctc gagcgcgaag gcccgcgcga
    13081 gacggcgctc gatctcctcc gccgggagcg tcatcccggg ttcccgaggg cttccgggca
    13141 tcggcatgta ctcgatgaag cgcacgtcga cgggcaggtc gcgggcgagg agggcgagcg
    13201 gcaggagctg gtcctcgttc tggccgccca cgatgacggc gttgatcttc acgcgcgaga
    13261 ttccgctcgc aagggccgcg tcgatccctg ctctcacggc atcgaggcgg tcccggccgg
    13321 tcatcgcccg aaacgcctcg cgcgtcgtcg cgtcgaggct cacgttcacc tgcgcgagcc
    13381 ccgcttccgc gaggggacgt gcgagacgcg ggaggagcac gccgttcgtc gtgagcgcga
    13441 cgagcggcgg ccgcggagcg aaggccgcga gcgcgcggac gacgggcacc aggccggagt
    13501 ggagcgtcgg ctcgcccccg gtgagtcgga cctttcgaac gccgagcgag acgaagagcg
    13561 ccgcgagacg gacgatctcc ccggtctcca tccggggcgg gagcgcggcg acccccgcct
    13621 cctccgcggg ccggcaatag gtgcagcgga ggttgcagct ctcgaggatc gagagccgga
    13681 ggtaggtgat gcgccggccg atcgggtccg tgagcccggc gtccggggcc ggtcccagcc
    13741 gttcggcccg ccgttccccc gggcgggagc agccggctgc ggcgggaagc ggcgccgcgg
    13801 gggactccat ccccaaacgg tagggtccgg aggtgcggtc tcctatgacg gccgtcatag
    13861 cgggcgcggt cgcgctcgca gatagtcgcg atcaaatcac tgaggagaac gcgatgagca
    13921 gttccgcgac gggccgggcc acacgcactt ccggctgggc cggccttctc tcacggattg
    13981 cggccggagc cctcgtctac ctcgtcgtga gcggcctgcc gctccgcctc ttcggcttct
    14041 cgccggtcct cgagtggacg gtcctcctcc acacgctggc gggcttcctg tcgcccgtgc
    14101 ccgtcggggt ctatctctgg cgccacttcc tcgagtaccg cgcggacgcg ctcacaccct
    14161 cgaaagttct cggctactgc gggctcggcg cggtcctgct cgcggaagcg acgggcttgt
    14221 gggtcaccta cctcgcgctc ttctcggacc gggtgccgcc tttcgcgcgc acgctccacc
    14281 tcgtcccgtc cgtcgcgctc ctcgtcctcc tcttcgcgca tctctggccg ctccggaaga
    14341 ggaccccggc cgagggcgtg gcccccgaaa cgttctctgc cgcgcgccgc ggctacgtcg
    14401 cggcgctgct cggcgccccg gccgtcgcgc tgcttctcgc gttcccggcg agcctcgtgt
    14461 acgacgggcc gaagacggca gggagctttc cgaaggacta ccggaagccc tacgggcagg
    14521 accggccctt cgctccgagc ctcgcgcgga cggagtcgaa cggccccctc gctcccgaag
    14581 cgctcgcggg ctcggcgtcc tgcggcgtcg cgggctgcca cccggcgatc tacgccgagt
    14641 gggcggtctc ggcccaccgc tgggccgcgc tcgacccggc gttccagcgg gttcaggaag
    14701 agatgggcaa gcagaacggg ccggagtcca cgcgctattg cggcggttgc cacgacccga
    14761 tctcgctgtt ctcgggcgcc aaggtcgtct tcaaggaaaa gctcaccgag gatctcggct
    14821 tccaggaggg cgtctcctgc ctgtcctgcc acgccgtctc gaagaccgac gtgcagggca
    14881 acgcgaacta caccgtgaag ccgccgaagc gctacctctt cgagaacgca aagagcgcgc
    14941 cgctgaagct cgcgtcgtac ttcctcatcc ggagctaccc gcgtcaccac acggactctc
    15001 tcgagaagcg gctcttcaag acgccggagt actgcgcggc ctgccataag cagttcgtcg
    15061 accaggaggt caacaacttc ggctgggtcc agctccagaa ccagtacgac aactggcgga
    15121 agagcaagtg gaaccatccg aaggacgcga agacgacggt ggagtgccgc gagtgccaca
    15181 tgcccctcct cgacgggccc gagcccgcct ccggcgacac cgcggactac aaccggaccg
    15241 cgacggacgg gaagcaccgg agccaccgct tcctcggagc gaaccagtac atccccacgc
    15301 tcctgaagct ggagggcggc gagcagcagg tcgacctcgt gaagaagtgg ctgaaaggcg
    15361 aggtcgagat cccggagatc gcgaacaagt gggcgaaggg cgccgtcgtc tcgctcgacc
    15421 tcgacgtccc cgcgaaggcg cgccggggcg agccgctgaa gctgcgcgtc gtgatcacgt
    15481 cgaacaaggt ggggcacgac tatccgacgg ggccgctcga cctgatccag tcgtgggtcg
    15541 aggtcgaggt gaaggacgag accggccgga tcgtcttcgc ctccggaaaa cccgacgaga
    15601 agaagatgat tccgcccggc acgttcctct tcaaggcaga accggtggac cggtacggaa
    15661 acctgatcga ccggcacaac ctctgggaaa tggtcggcgt gaggtaccgc cgcagccttt
    15721 ttccgggctt ctcggacgcc gccgagtacg cgttcggctg cccgggcacg ccggcgggcc
    15781 cgagcccgac gcgcgacgtg acggttccgg cgccgccgcg cggaaagctg gacgtcctcg
    15841 cgacgctgca ctaccggaag ctggaccagt acctcgtcga cttcctcttc ccgggcaagg
    15901 gcctctcggc gccgatcacc gacctcgcgc aggtgcggac gacggtcgag atcgtcgact
    15961 gacgtggccc ggcccgttct gaagcgctgg acacaccggc gcgtcctcgc ggccgccggg
    16021 gtggcggccc tcggcgcgtg cgcgatcctg tggttcacga agaaggacga ggacgcgccc
    16081 tacacgccgg gagccgccgt cgagggcgtc acggacgtcc tcaagaagga cgcctccggc
    16141 gcggggtggg gcgtcgtctt caaggacgtg gcgcagagtc gcgggatcga cttctcgcac
    16201 ttttccgcgg gccctcgctc gacgcagctc cccgaggaca tgggctcggg ttgcgcgttc
    16261 ggcgactacg acggcgacgg cgactgggac ctcttcgtcg tcgacacgat cgggccgctg
    16321 acgctgacgt ccggcgatgt gcgcgcctcc aggggaggct gccgtctctt ccggaacgac
    16381 ggcggcacgt tcacggacgt gacggcggag gcggggctct cggacctcaa gggaacttac
    16441 atgggcgccg cctggggcga ctacgacaac gacggcttcc ccgacctcgt cgtgacgtcg
    16501 tacggaggga ttcgtctctt ccacaacaag ggcgacggca cgttcgagga ggtcacgaag
    16561 gtctccggcg tcggcgcgtt caagggcttc tacacgggcg cggtgtgggg ggacgtggac
    16621 ggcgacggcc tcctcgacct ctacgtctgc acgtacgtcg actaccggtt cgacccgaag
    16681 gacgcgggca aggcctcgaa gttcgggcag gccgtgagcc ccttcacgat caacccctct
    16741 tcgtatccgc cggccccgaa cctctacttc cacaacgagg gaaaggggcg ctttcgcgag
    16801 atggccctcg aggccgggat cgcgaacgcc gaagggcgga gcctctcggc cacgttcgcc
    16861 gacctgaacg acgacggcaa gcccgaccta tacgtggcca acgacgtctc cgagggggcg
    16921 ctcttcgtga acgagggcag cggcctcttc tccgaccgtg ggcacgaggc gcacgtggcg
    16981 gattaccggg gagcgatggg gatcgcggtc ggcgacgcga acggcgacgg cgcgccggac
    17041 ctcttcgtca cgcactggat cgccgaggcg aacgccctct acctgaacaa gctcggaggc
    17101 gggaagagcc ggacgctcga cttcgaggac gctgccgaga ggaccggcct cgggcaggtc
    17161 tcgacggacg acatcgcgtg ggggacggcg ttcctcgacg tggacggcga cggactgccc
    17221 gatctcgtcg tcgcgaacgg ctccacgttc gaggagcccg ccgacacgcg gaagctcgtt
    17281 ccgatgccga tgcgcctttt ccggaacctc ggcgagaagg gcttcgtgga cgtggcgccg
    17341 ctctccggcc ctgacctgac cgcgccccgc gtcgggcggg ggctcgcgat cgccgacgtg
    17401 gacggagacc tccgggagga gatcgccgtc gtcgtgaacg gcgggaagct cgtcctcctc
    17461 aaggccgagg gagggccgcc gaaccaccgg atcgcgatcc ggtgcgaggg ccggaagtcg
    17521 aaccgttccg cgttcggcac gaagctcgtc ctcgaggcga acggcgggcg gcaggttcgc
    17581 gagatcggcg cgggctcctc gtacctgtcg cagagcgcgc cggaagcgat cttcggcctc
    17641 ggcgctgcgg cgaaggccga cgccctgacc gtccgctggc cttccggcgc cacagccacg
    17701 ttccgcgacc tcgcgggcga tcgcgcgtac ctgatcgtcg aaggggaggc gtcaccgcgc
    17761 gcgctcccgg acaaccgggc gaaaacgctc gcgttctggg acgcctacaa cgccgcgcgc
    17821 gcggcgttcg cgaagggcga cgtcgccgcg tcgatggcgg cctaccgcaa ggcgctcgag
    17881 atcgacccgc ggcacgagga ctgccggtac tcgctcgcga acctgctcct cgaaaagggc
    17941 gaccgcgcgg ccgcgaaggc ggagctcgag acgctcctca gggtccagcc gaagagccag
    18001 cggggccacg gcgcgctcgg cgacctcctc gcggaccccg cttcggggcc gctcatggat
    18061 ttcgcggctg cgcggaagca ctacgagagc gcgggcggga tctactcgcc ggagacaggc
    18121 tgggtcgtgc ggaccggcga ggtggctctc gtcacgggcg acgcggcggg cgccgagcgg
    18181 gcgttccgga aagtgctcgt ctcgaatccc cgttccttcc aggcgctgta cttcctcggc
    18241 tacctcgcct cgcggcagaa gaagaacgac gccgacgggg cgcgcctctt cgacgaggcg
    18301 ttcgccgcgc tcggtccgca ggcgtcgccg gcgccgggcg aaggcgacgt caagaaggcc
    18361 gaaaaggcgc tcccggcacg tggcgcgttc gcggggcact ggggatacct cgggcagggt
    18421 gcggtgaaga aggacgacgc ctggcgggac ctcgacgggc ggatctcgcg cggggcgccg
    18481 aagcggccct gaatcgtcgg gcagcccggt tcaggccgac aacgccacga cagcccgcgc
    18541 gacgacggcg aagtggcagc cggccgccgc gatcacgagc gcgtggaaga tctcgtggta
    18601 cccgaagatc ctcgggaacg ggtcgggccg cttgaccgcg tagacgaccg cgccgaggct
    18661 gtagacgacg ccgccggcgg cgaggagcac gacggcgccg gtcccgagcg cggccttcag
    18721 cgccggcagg agcggcacgg ccgcccagcc gaggaggacg cagacgacgg cgacgagggc
    18781 cttcggcgcc ttcacccacg cgacggacat cacgatgccg agcagggccc ctccccagac
    18841 gatgccgagg aagatccagc cgaggcggct gccgagcagg aagctcaagg gcgtgtacgt
    18901 cccggcgatc aggacgaaga tcgcggagtg atccagccgc cgccagatcg cgcgggcctt
    18961 ctccggccag ttccggcggt ggtacgccgc gctgacggac agcagcgtca cgagggagaa
    19021 cacgtagacg agcgcgcccg cgcgggcgcg cgccccggag gcgcggaaga agaggatcgc
    19081 ccccgcgacg gccgcgacga acgcggccac ctcgtggagg acgccgcgga gaaggggctt
    19141 ggggcgagac gagatctcag gaggttccat tccacggacg attctgcacc cgacgcggcg
    19201 gcgcggcgag ccgtcccccg agacgtcgtc agacgaggcg ctggatccgg gcgacgagct
    19261 cgtcggcttc cgacgctccg agggcccggt ccgcgagcgg atagaggatc tgctcttcct
    19321 tgaggttgtg gccgccgagg acggagagca tccgcgcgcg gatcgccggc gcgccggcgc
    19381 cgccgccgtc gagagccgcc gcgattcccg cgagacaggc ccggatctcg acgtgctcgg
    19441 cgcgcatgac ggccgtgggc ccggcctcgg gtcggaagcc ggcgcgctct tcgagcgtcg
    19501 ggaagaggat ctcctcctcg aagcggatgt gccgacccag gccccgcgcg aatgcggcga
    19561 acactgcgcg agctcccgcg acgtcgtccg ccgcgagcgc ggagaaggcg gccccgtcga
    19621 gggcgtccag ccggtcgtga tcccaggcca gcgcctcgaa gacgccgcgg gacgtgccgc
    19681 tcgcggcgtc gcggcggaga acctcgatcc cggcgggtcc ggcggcgaag cctgctggcg
    19741 tccactcgaa caggcccctt cgttcgcacc gcagcgcttc gaggaggtcg agcgccggcg
    19801 cttccgccag gacgagcgtc tcgccgggcg cgagggcgtc gaaacgggcg aggagcgcgg
    19861 cggcgcgcgc gggcgcgtcc gctccggcga ggtcgagggt cgatacggtc cgtgtcgcgg
    19921 tctgggccat ggcgtcctcc acggcgccga tgatttcgcg tctgagtggc gcccgacatg
    19981 acggccgtca tgtcggaccc gaatcggtga ccgagaatcc gcttcgggag gtccgccatg
    20041 atcctgaatg ccgcccggtc cgccgcgctc gtcgccgccc tcgccgtcct cttcccgggg
    20101 cgcgccgccg cacactgcga ttccgtggac ggtccggtcg tcacggacgc gcgtgccgcg
    20161 ctcgagaagg gcgacgtgac gccggtcctg aagtgggtcg cgcccgaagg cgagaaggag
    20221 atccgggcgg cgttcggccg cgccgtcgcc gtccgcaagc tcggacccga cgcgcgggag
    20281 ctcgccgaca cgtcgttcta cgagacgctc gtgagggtcc accgcgccgg ggagggagcc
    20341 ccgtacacgg gcctgaagcc cgcgggctcc gtcgagccgg cgatcgcgaa ggccgacgcc
    20401 gcgctcgccg ccggctccgc cgacgacttc gcccggctga tcgccgccca cgcggccgat
    20461 ggcctgaaga cccggttcgc gcgcgtcgtc gaggcgaaga agcacgccgg ggagagcgtc
    20521 gccaagggcc gcgagtacgt cgccgcctac gtggattacg tccactgggt cgagggcgtc
    20581 gtgacggcgg tccatcgggg ccccgccgcg cacgagggcc acgcggcggc cgcggcccac
    20641 gtccactgaa cgcgttcagg ccgtcgtctt ctggaagcgc gagacggcga ggccgatgat
    20701 cacgaccgtg tagaacgtca gccaggcgac gggctcccag agctccgtca gccccgcgcc
    20761 gcgcaggacg atgccccgga ccacctcgat gaagtacttc gccgggatga ggtacgagac
    20821 gtaccggaag aacacgggca tgccggcgat cgggaagacg tagcccgaga ggaagacgaa
    20881 cggaagcagg aagaagaacg acatctgcat cgcctgcatc tgcgtcttcg cgagcgtcga
    20941 gagcagcatc ccgaggccga ggaccgcggc gatgaacatg aggcccacga gatagagctc
    21001 gaggacgctc cccgcgatcg gcacccggaa caggtagcgc atcaggacga tgatgagcgt
    21061 gagctggaag acgccgatca cgacgaacgg cgcgatcttc ccgaggatga tctcgacgcg
    21121 cgtcacgggc gtgacctgca gctgctcgag agttccgcgc tccctctcgc ggacgatcgc
    21181 catcgccgtg aactggatga gcgtgaacgt gaggaggatc gcgatgaggc ccgggatgat
    21241 gaacgtggcc gtgcggaggt cggggttata ccagggcctc acgcgcaggt cgacgggcgg
    21301 gccggactcc ttccagccgg ccctggcggc cagcgtccgg acggacatct ggttcgagat
    21361 cccgcccgcg atcgccatcg cctggctcgc ggtcgtcgtg tccgaggcgt tcacgacgag
    21421 gagcgccttc gcggaggccc cgcggcgccg gtccttcccg aagtcggcgt cgatcacgag
    21481 gccgacggag gcgtggcccg cgtcgatctc gtggcgcagc gcggcctcgg agccgacgta
    21541 cttcaccacg tcgaaatact cgctcgccgt catcttcgcg acgagctcgc ggctgtccca
    21601 ggaccggtcc tcgtcgagga cgaccgtctt caggtgcttg acgtcgtagt tgatcgcgta
    21661 gccgaagatc atcgtctgga ggatcggcac catcacgatc atgcggagcg tgatcgtgtc
    21721 ccggcggagc tggaggaatt cctttcggac gagggcgagt aggcgctgca tcgttcctcc
    21781 gtcaggccgc cgccgtgcgc gcttgcgggg tttcgcgccg ggccagggac acgaacacgt
    21841 cctcgaggga cggcgcggcg gcggccggcg ttccgtagcg ggagaggagc cgcgcggccg
    21901 cgtcggcggc gccctcgtgc gcgacggctc tcaggcgcag gccgaagagg taggcgtcct
    21961 cgaggctcgg gtctccctgc acggaggcga gcgccgcgaa cggctcggag gcgatcggga
    22021 cctcgaggag gcggccgtcg agggacgctt tcagctcggc cggactgccc gacgcgatga
    22081 tcctgccgtc gaggatgaag gcgagcccgt cgcactgctc ggcctcgtcc atgtagtgcg
    22141 tcgtgaccag caccgtcatg ccgcgcgcgg ccgcgagctc gtagatcagg tcccagaaca
    22201 cgcgccggcc cttcgggtcg acgccgccgg tgggctcgtc gaggaagagg agctcgggct
    22261 cgtggaggag cgccgcggcg agcgcgacgc gctgcctttg cccggtcgag aggttctccg
    22321 tcagctcgcc caggatcggc tcgaagcgaa gacgggcggc gagttcccgt atgcgcacgg
    22381 attggcgagc ggtcgaaaga ccgtagatgg acccgaagaa cgcgaggttc tcccgcaccg
    22441 tgaggtcgag atagagcgag aacttctggc tcatgtagcc gaggcgcttc ttccaggcct
    22501 cggtgtcccg cgtgacgtcg aggccgccga aagccgtcgc cgtgccggac gtcggctgga
    22561 ggagcccggt gagcatccgg atcgtcgtgg acttgcccga gccgttcggg ccgaggaagc
    22621 cgaacacctt cccgcgcggg atcgcgagcg acacgcggtc gaccgccgtg aacgcgccga
    22681 acttccgggt gaggtcgtcg cagcggagga ggatctctga ggaagaagaa gacgaagaga
    22741 agactttctt agaaggtgaa gaacgctcgc cctctttacc ttctaagaaa tctttgtctt
    22801 tctctttctc tttctcttcg ctgaagaaaa tgaaggcgtc gtccagcgtc ggcgcgactg
    22861 cctccgctcc gcctcggtct ctgggcacct tcgagagaat ctcctccttc gtccccgtcg
    22921 ccacgacgcg gccctcgtcg aggaacgcga ggcgggtggc gtactcggcc tcgtccatgt
    22981 agggcgtcga gacgacgatc gtcagcccct cgtggtggag cgcgttcagg acttgccaga
    23041 actcgcggcg cgagacgggg tcgacgcccg tcgtcggttc gtcgaggagg aggagcgccg
    23101 ggcgcgtgag gagcgccgag acgagcgcga gcttctgctt catgccgccc gagagcgcgc
    23161 ccgcgaggcg cgagccgaag cggtcgagcc cgacgcgggc gaggaggtcc cgcgcgcgtg
    23221 cggcggcctc ggcggagggc acggagtaga gccgggcgcg cagcgcgagg ttctcgtcga
    23281 cggtgaggtc ctcgtacagc gagaagcgct gcgggacgag gccgaacggg acgtcgtcgc
    23341 ggacggcgcg gcccgacgtc ggctggagga gacccgcgac gatccggaag aacgtggtct
    23401 tgcccgcgcc gtcgggcccg atgagcgcga acatctcgcc gcgcgcgatg gagaggtcga
    23461 tcccgcgcag cgccgggcgg ctgccgaagc tccggacgag cttttcggcg cggatcactt
    23521 cggggcgctc tcggcggtct tcacgcgcac gtcggcgggc tggcccggga cgagcggcgc
    23581 attccagccc gcgtcgaggt ccaccttcgc ggcgaagacc atgttcacgc gctcgcgcga
    23641 cgtgtcgacc ggcttcggcg tgaactcggc gtccggagag atctccgtga ccttccccgg
    23701 gaacgtccgc cccttgaacg cgtcgacgga gacctcggcg gcgctcccga cgcgcacgcg
    23761 gccgagctgc gtctcgggga tgtacgtgcg cacgtagagg cggtcgagga acgccatcgt
    23821 cacggcaggc tgacccgctc cgaggaggag gccgggctcg gccatgcggt ggaggatgac
    23881 gccgtcggcg ggcgcgcgga tctccgcttc gcgggcgacg gactcggact gcttgagcac
    23941 ggtgcgggcc cgtgagacgt cggcgcgcgc ctgctcggtc tggtactggc ggaagccctc
    24001 gcggaggagc tgcagtcgat cgctggccat ctttgcgttc gccaccgccc gttccatggt
    24061 ggtgcgggcg gtgtccgtgt cgcgctgcgt tccgactttc ttagaaagta agacctcctg
    24121 gcgctccagc tctcgtcgtg cgagttcaac ggcggccttc ttgtcggcga cgtccgcctc
    24181 ggcctgagca atctcggccg cccggcttcc gaccttcagg tcgttgaagc gcgcttccgc
    24241 ggactcgagg cccttcgcgt cgcgctcggg cgcgagcgcc gtctcgccga ggtccagtct
    24301 cgcgacgagg tcgcccgcct tcacgcggtc gccctccctc accttcacct cgacgacgcg
    24361 gcccgcgact ttcggcgcga ggtcgaccat gggcgcctcg agccgtccgc tcaggacggc
    24421 gacgttcgga tccccgccgt tccggcaggc ggcggcgaga gcggcgaagg cgaggacggc
    24481 ggagcgaggc attcggggca tgggccctcc taggaggcgg ccggcgccgg ccggctctcg
    24541 gccgcgggcg tcgcggcagg ggcgtgctgc ttgcgcatcc ggcggacgag ccgcccggcg
    24601 gcctgctggg cgtcgtcgaa gagcgagtag gcgaccggcg tcgcaaggag cgtgaggagg
    24661 agcgcgagcg tctggccgcc cgcgatgacg gagccgatcg cgcggttcgt gccggagccc
    24721 ggaccggagg agaccaggag cgggagcatg ccggcgacga acgccatcgt cgtcatgagg
    24781 atcgggcgga ggcgctcgcg gcaggcaagc gtgacggcgg cgtcgcgctc catgccgcgc
    24841 tcgcgaagct ggttcgcgaa ctcgacctgg aggatcccgt tcttcttgac gacgccgaag
    24901 aggacgagga tcccgagcga cgtgaagatg ttcacgctct gcccgaagat gaggatcgag
    24961 aggagcgcga acggcacggt cagcggcagg gcgaggagga tcgtgatcgg atggagccag
    25021 ctctcgaact gcgccgcgag gatcaggtac atgaagatcc ccgagagcgc gaacgcgagc
    25081 aggaagttct gtgccgaacg gccctgctcc ttcgagcgcc cgacgaaccg gtacgtgtag
    25141 gacgcgtcga gcttcagcgc cttcgtctcc ttcacgaggg tgtcgagcgc ggcctgtgac
    25201 gaaaagcccg ggttcatgtt cgccatgatc gtgacctggc ggcgccggtt caggcggcgg
    25261 atctccgacg ggccgacgcc ccgccggaag gacgcgacgt cctcgagcgg gacggcgccg
    25321 agccggtagg acgggacgtt gaaccgcttg aggctcgcct cgtccgcgcg gtcctcggag
    25381 cgcgcccgga ggtagacgtc gtactgctcg cccccgtcgt cgaaggcgga gactttctcg
    25441 ccgccgacga ggacgcggag cgtctcggcc acgtccgcga cggagacgcc gaggtcggac
    25501 gccttcgagc gcccgaccgt gacgccgagc tcgggcttgc cgaagatgag cgtcgagtcg
    25561 acgtcgacga tgccgggcac cttgcgcagc ttctccatga gcccttccga gaccttcgcg
    25621 agcttgtcga ggtccgggcc gttgatgacg tacatgatct cggcgttctt ctggccgctc
    25681 gagaaggcgt tcacgtagga gacgtccgtg cggagcttcg cgtccgcgta ccgcggcagg
    25741 atctccttgc ggacgcggtc catgaccgtg gtctgggact ccttccgctt gccggcgtcc
    25801 acgagcttca cgtagatgct cgcgacgttc ggcgtcttcg cgtcgtccgc gccgaccgtg
    25861 aggagggcgt actcgacgcc ggggagcgtc ttgacgtcct tgctgatccg gttcccgatg
    25921 agcgcggtcg cctcgagcga cgtcccttcc ggcgcgcgga agttcacctc gaactgcgac
    25981 tcgtcgtcgt aggggaggaa gttcttcggg acgaccttga agaggagcgg gaccgtgagg
    26041 agagcgacga ggcagagggc gacgatggcc cagcggtggg tcatcgacca cttgagcatc
    26101 gccgtgtacg tccggtcgat ggcggcgtag aagccgcggg acttcgacga ggcaccgtgg
    26161 ccggcggccg ccttcttctc gtcgcgcttc aggaaccgcg ccgacagcat cggcgtgagg
    26221 gagaaggaga cgaggaggga gacgaggatc gcgaacgcca tcgtgaggcc gaacgagttc
    26281 atgaaccgtc cgacgatgcc gcccatgaac gcgacgggca ggaagaccgc cacgagcgag
    26341 agcgtcgtcg cgaggaccgc gaggccgatc tccttcgtcg cgtcgatcgc cgcctggaac
    26401 ggcatgacct tcttctcctc gatgaaccgg aagatgttct cgaggacgac gatcgcgtcg
    26461 tcgatgacga tgccgacggc gagcgtcagg gccaggaggg tcaggacgtt cagggtgaag
    26521 cccatcgcgt acatcagggc gtaggtcgag acgatggacg tcgggatcgc gatggccgcg
    26581 atgagcgtgg agcggatgtt cccgaggaag aggaggacga cgagggccgc gaggaagccg
    26641 ccgacgagga ggtgctcctg cacggtgtcc acggccgcct tgatgaacat cgactggtct
    26701 cgcacgatct ccgtcgaata ccccgcgggg agctgcgggg cgatctcggc gaggcgcccc
    26761 ttgagcgcgt cgatgacggc gatcgtgttc gtgccggact gcttgcgcac ggagaggacg
    26821 accgtcggga cgccgtcctt gctggcggcg cgctcggcgt cctcctcgct gtcctcgacc
    26881 cgtgcaacgt cgccgacgcg cacgggcagg ccgttgcgct gggtgatgaa gatctcctcg
    26941 aactccttcg cgttcgcgac gcgcccgtag acgcgcacgg tcgcctcgtt cgagccggcc
    27001 ttcagcgtgc cgcccgggat cttgacgttc tgcgtctgga gggcgcggcg cacgtcgacg
    27061 gccgagagcc cgtaggcccg gagacgctcc gtgtcgagcc gcacgttgat gcggcgcgag
    27121 cggccgccga ggatcgtgac ctggccgacg ccgttgacgg actcgatctg ccggcggagg
    27181 accttgtcgg cgtactccgt agtctcgcgg agcgtgcgct tgcccgagag cgcgaccgac
    27241 aggatcggcg tggcgtccgg gtcgagcttc tcgacgatgg gctggtcgat gtctcgcggc
    27301 agctcggcga ggacgcggtt gaccttgtcg cggacctcct gcgcgcccac gtcggcgttc
    27361 ttctcgagga tgaacgtcac gaagacctgg gagatgccct cggcggacac ggactgcagg
    27421 tcgtcgatgc ccgcgatcgt gttgatcgac tcttcgatct tgtccgtgac ctcggtctcg
    27481 acctcctcgg gcgcggcacc cgggaggcgc gtgatgacgg cgacggtcgg gaagtcgacc
    27541 ttcgggtagc ggtcgacgcc gaggcgcgtg taaccgacac cgccgacgac gatgaaggcg
    27601 atgatgagga cggacgcgaa gacgggccgg cggacgcaga gacgcgcgag tgcctgcatg
    27661 acggattcct cagcgggccg cggcggagcc gcccgggacg acatccagcc cgtccacgag
    27721 cctgcgatcg gggttcacga cgacgcgctc gccggcggcg acgccgctgc ggatctcgac
    27781 ggacgtgccg tagcggtcgc cgagggcgac gatcctctcg gagacgcggt ccttgccgag
    27841 gacgaagacg tgcgacacgc ccgagtccgt cacgatcgcc gccgacggga cgaggagcgt
    27901 cggttcgttc ttcgggagct cgacgcgggc cgtcacgaag aggccgggct tcaggatgcg
    27961 ctcggagttc gggacgacgg cctcgacgac gagcgtccgc gcctcgctcc gcagggacgg
    28021 accgatgtaa cggacgacgg cgtcgaacgt gcggtcgggg aacgactgga cgttcaggcg
    28081 gcccttctgg cccactttga tgaacgggac ggcgacctcg gggatcgcga gctcggcgcg
    28141 gagggggtcg atcctcacga gaccgcagat ggcctgcccg cggcggacga acgcgcccgc
    28201 ggagatcttc ttctccgtca cgacgcccga gaacggcgcg cggatcgtgc agtcctcgaa
    28261 ccccttctcc gcgagcgcga ggttcgcctt cgcgaggtcg aggtccgtgc ccttccgctg
    28321 gtcctcggcc ttcgcgacga cctgcttgcg gcggagctcc gcgtagcgct cgacttcgga
    28381 cgacgtccac gcgaggttcg cgcgggcctg gtcgagctgg gccttcgcct cgcgctcgtc
    28441 gagcgtcgcg aggacggcgc ccttctcgac gtacgtcccg cgttcgaccc gcaccgtcgc
    28501 gacctggccg tcgcgctcgg aagcgacgtc ggcggactcg tcggccgcga gcgcccccgt
    28561 gacctcgagg atgcggggca ggacccggtt ctcgaccggg gccgtcacgg ccgggatcgg
    28621 agggacggct gcggacgcct tcgaagccgc gttcgggcgc gagcaggccg cgagcaggag
    28681 cgttccggac agcagaatac cggcggactt cggattcatg aggttcagcg tccttccgcg
    28741 gccctgaggg ccggagcgtc gcccgcgatc tcgatgccgc gctcgtcgag gagcgtgccg
    28801 gcggtgcggg aatagtcggt cagggccttg cggtagtcgg tcagcgccgc gatctccgta
    28861 aggacggcgg tcgcgagctc gttctggcgg gtcaggacga agaagttcgt cgagaggccg
    28921 acgttgtagc gctctttctc ggcggcaagc tgcgtctcgg cggcggaacg tcccgccttc
    28981 gcggcgtcga tgcgctgcgc cgccgtctcg agcgtgagga ccgcgtttcg gacctcgacg
    29041 gcgacgcgct gtctctgctg cgaaaggagg atctcggcct ggctcttctg cgccttcgcg
    29101 atcgcgacgt cgcccttcgc ggagcggttg aagaccggca ccgtgaggga gaggccgatc
    29161 cactcgtcgg ggaagcgcga ctcggtgatc gtcccgagcg agcggccgaa ggagccttcc
    29221 agcgcgtccg gcacggcgac cggcgcgccg aggaagccgt tcgacggggc gttggggttc
    29281 agcgcgcccg cgagtccgcg gcgcgcccag cccgcgacga cgtccagctg cggcttgagg
    29341 cggtcccgcg tgtagtcgac ctccacgttg cgctgcgcga cgcgggccct tgcttcggcg
    29401 atctcgggcc gcttgtcctc ggcgacccgg agggcggtcg cgaggtccgg gcggacgaga
    29461 ggcgtttcgg gcctgtcgac ggggtcgagc gtctggttcc agagggggtc cgactcgtcg
    29521 ttcaggagga gaagcttgag aaggagctcg gcgcggcgcg cattttcctg cgacgcatag
    29581 aggtcgccgc ggcggcgctc gtactcggcg atcggctgcg cgatgtccga ctcgggaagc
    29641 gttccgacgt cgatccgcga cttcgtatcc tcgcgctgct cggcggcgag ccgcatgctc
    29701 gcctcccgga cgaggacgtc gcgccgggcg gcgacgagcg tccagtacgc gcgctcgacg
    29761 ctcgcgaccg tgtccgtgac cgtgctgcgg agcgacgcgg tgctgcgttc cttctcgagc
    29821 gaggcgatcc ggattgcccg gcgggccggg tcgatcgaga ggttctgcag gagaggctgc
    29881 cggatgtcga tcccgatcga cgtcgagtac gccggcgaca ggagcgcgag aaagttgttc
    29941 gtgaggtcgc gcgcgccgta ggcggaaacg gcgatggtgc cgcccgtcgg gagaagggcg
    30001 ccgattcccg cggcgcccga cacgccctcg gacgacggag agatctctcc gtacggagct
    30061 cccgagagga gcgaattcgc ggggtccgtg tggttgcgat agcgcgcgtc gagctggaac
    30121 gtcgggtcat acgagccgtc cgctctcagg atggaggcgt ccgcgatccg gaacgactcg
    30181 cgctcgaggg cgatgtcgtt gtt
//
LOCUS       DAOMFY010000016        29360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_507_length_29360_cov_7.986589, whole genome shotgun sequence.
ACCESSION   DAOMFY010000016 DAOMFY010000000
VERSION     DAOMFY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 29360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29360
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_507_length_29360_cov_7.986589"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PLB01_02720"
     CDS             complement(<1..928)
                     /locus_tag="PLB01_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ76243.1"
                     /translation="MADPESHRAIDAIWRIESPRLIATLVRVTGDVGLAEDFAQDALV
                     AALERWPEAGVPDNPGAWLTTVARNRAIDHVRRGRRVERAHGEIGRDLEAERERAVAD
                     LDAALDDDIGDDLLSLVFTSCHPVLSREARVALTLRLLGGLTTAEIARAYLVPETTIA
                     QRIVRAKRTLADARVPFEVPRGPDRDARLSSVLEVLYLIFNEGYAATAGDDWMRPQLC
                     EEALRLGRVLAELAPAEPEVHGLVALMEIQASRAKARLGSGGEPVLLLDQDRGRWDYL
                     LIGRGLAALARAEALGGRGPYALQAAIAACHAR"
     gene            complement(965..1390)
                     /locus_tag="PLB01_02725"
     CDS             complement(965..1390)
                     /locus_tag="PLB01_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQQ76244.1"
                     /translation="MRFLVMVKATKESEAGVMPSEKLLADMGKFNEELVKAGVMLAAE
                     GLQQSSKGVRVRFSGSQRTVIDGPFTETKELVAGFWLWEVKSMEEAIEWVKRCPNPHE
                     GESEIEIRKVLDVSDWGELTPELEKMKERREKFAERSKT"
     gene            1565..2536
                     /locus_tag="PLB01_02730"
     CDS             1565..2536
                     /locus_tag="PLB01_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76245.1"
                     /translation="MSTNRNVFLTALLAALAATPLPAPAESACEAVGAADFGALGFGT
                     MGAGSGRKPAYVVKLDSNAAISGLETECLWNSGPGSLTLLLGDMDANLVKGDDVRRLG
                     QKVLAAVREFHETAGKYSGRPMTIAPLPGVGDEAYSARLDAHFFEVWGRRGLRLARLK
                     ISAPPSMKRSPTTEQAGALLGRILSHGSGYTTSGAPAIQGESPWSGPGDKYVVTGSIV
                     ATGALSGTFTWSSPGEVETYPDRTEIVLATADKSAYMNLQVWNGEDRVEVRSGKLRSG
                     FLRGSGRVAKVDPLAGSGEIRVDATVKSATETITVKGALTVRPARKK"
     gene            2562..2777
                     /locus_tag="PLB01_02735"
     CDS             2562..2777
                     /locus_tag="PLB01_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76246.1"
                     /translation="MTGSGRERGGPVGLGCGLLLGLAVGAAWVVLDGFPPWTFVVPAV
                     SLAAVAYFYGDRFWEWLAEHGWKVPWF"
     gene            complement(2788..4824)
                     /locus_tag="PLB01_02740"
     CDS             complement(2788..4824)
                     /locus_tag="PLB01_02740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ76247.1"
                     /translation="MTIARRLSTLAACLLLAVALPLAADEAAAKKRFSVDDFEKIVRL
                     SSPRISPDGKSIAVVVTRADVAKNKWLSELSLVDVSTGALRPLTHDRKGVSHPRWSPS
                     GERLAFLAADGAGKDAKSQIFVLPMGGGEAQRVTNAPTGVQHFAWRPDGKAFAFAAED
                     ERPNKAEIEKGNDLFEVGSDSLYVSEAAMPVHVWLVPAEGGEAKRLTSGAWSLPAALP
                     PSPPSSPLSWSPDGKSLAFVRVETPHSGDNDAATVRVLDVESGSIRALTGKTHFEGFP
                     VFSPDGSKISYWCPRDRDPNNVNDVHVAPASGGAGVNLTKPIDRCLYASVWTPDGKAL
                     LTGGNDGTRVSLWLAPLDGPAKRLELGDVNPAWSFFVDVSAGPNGAIAFVGSLPDRPG
                     ELWWMASPSSAPKRLTDLNAEIAGRDFGKVETFTWKGPDGFDEDGVVVLPPGLAPGAK
                     VPLVLYIHGGPQAASTRAFPAPAHLFAAQGWAVFMPNYRGSDNRGNAYQRAIFNDAGD
                     GPGRDVLAGVDALVKKGFVDASRIAVSGWSYGGYMTSWLIGHDTRWKAAVAGAPVTDL
                     ADQYAFADFNVQMRYAMKGNATPFSPGGDALYRAQSPITYATRAKTPTLILSDTGDFR
                     VPYTQAFKLFRALKGAGVETSFVAIPVEGHFPGDPVRTREVFRRWVDWLAPRLK"
     gene            complement(4897..5208)
                     /locus_tag="PLB01_02745"
     CDS             complement(4897..5208)
                     /locus_tag="PLB01_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76248.1"
                     /translation="MGRLVFAFLSRGDPDGIAAREREISVALHATPCVHGIGGAWLTP
                     SKCPQCMALIEASQAAARARMRATREAQFVREVREELGRREAVQRTRPSRGTSFDRPA
                     G"
     gene            complement(5555..5731)
                     /locus_tag="PLB01_02750"
     CDS             complement(5555..5731)
                     /locus_tag="PLB01_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76249.1"
                     /translation="MKIGDEKTPCTCGHARDEHRGTISDVGGLVGSYGLECLVAGCDC
                     PRYAPDDEDEDSDA"
     gene            complement(5737..5880)
                     /locus_tag="PLB01_02755"
     CDS             complement(5737..5880)
                     /locus_tag="PLB01_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76250.1"
                     /translation="MAVGNMFVANGQSYTTYTPEHAAGYTAYRMFWSNSDGVIYRWQW
                     KGL"
     gene            6618..6848
                     /locus_tag="PLB01_02760"
     CDS             6618..6848
                     /locus_tag="PLB01_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQQ76251.1"
                     /translation="MNQPPAAPLLLDENEAERYSGISKYTLRGYRRQRRGPIVVKLGR
                     SVRYRPEDLSAFIAENVIPPGDMPKVRRRPCR"
     gene            6839..7357
                     /locus_tag="PLB01_02765"
     CDS             6839..7357
                     /locus_tag="PLB01_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76252.1"
                     /translation="MPVSVPPDHAAARVALDDGERLARILEFARTDTGALLRDADSRR
                     AARSPEERRAAAEAALRTRRQAARAVVGDRAPEWLAELVAHRGTESVFGGPDGALLKL
                     ANGIAFGEERTVALRQLADYLLACGVSAPMALTFVGAWANTFAAPVPNEQEILQAVAR
                     VVEQRQRTRRRA"
     gene            7354..8502
                     /locus_tag="PLB01_02770"
     CDS             7354..8502
                     /locus_tag="PLB01_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HQQ76253.1"
                     /translation="MRDALEVIAGSEYGQLIAPPDADAPLSVVDLLRRVYVGDRFPTG
                     LAALDERLKGGLAPGFLFVIGGEPWIGKTTLLVQIADALAADGVAVFIWAADEPPEGL
                     ALRLGQLHGEEQHELNPNYPPVLARLAGELSNGPRFLPESVETVEQAADFVERHTPDG
                     RVGALLLDSLQSQSERSGDDADSPRAALSGLMAELVEAKKRGLIVGAVSELARGAYAS
                     PDPGQRTRGLAAFAESRAIEYRSDIAMIMTSDGGGNVRLEVVKNRLGHLKGGLTLKLN
                     HARARFGAIADEVLKRQADDERQRVEQERLRADEDRVAGVLEKAERGLTKDEIRDRAR
                     MKKQAVGEALVRMEDDRPPRVRRFKPPREPKQSGPVPERFELPRGLGV"
     gene            8684..9145
                     /locus_tag="PLB01_02775"
     CDS             8684..9145
                     /locus_tag="PLB01_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76254.1"
                     /translation="MSRRCEGCRHPEAARIASLLASGESFRDVAKQVGLSASAIHRHR
                     AHAEIRSAIVRADEILKAVPVGPAHTDVREYMADARRLAEKAESERDYRGALAAVKIL
                     TDVALRLEERSSGQPGGLVTIEFDFSAMQPHRLAAPATEPPAGQSDEGSDE"
     gene            complement(9153..9845)
                     /locus_tag="PLB01_02780"
     CDS             complement(9153..9845)
                     /locus_tag="PLB01_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQQ76255.1"
                     /translation="MASKKAYAYLRVSSRGQVGGHGFGRQEEAIRAYAKAAKVEIAAV
                     YRDAHTGTEADRPAFVEMLAAILSNGVRTIVVESLDRLARDMGVQIALLGELRKRGVA
                     LIAANTGEDVTTTDDPMREAIIGMQGLFAQLDKKLLVRKLRAAREAKRNAEGRCEGRK
                     PFGSQPGEDAVLHRILTLRRGNRAHGRMSFAKIAETLNAEGIPTRTGTNWDPSVVFGI
                     VRRVSPKLTASK"
     gene            10512..10907
                     /locus_tag="PLB01_02785"
     CDS             10512..10907
                     /locus_tag="PLB01_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76256.1"
                     /translation="MIAPDTTDAQSFASNLKLLTDLLPAMHEPARRRAAWRVARGRAD
                     LAAAVAAEGYRLDGATAAPSPKAAPSTADAIAVLRHAQAVAQNAGAPPADVAAIRNAA
                     SGRIHAAALELQKRDPRLSYEAAVVEVTK"
     gene            10904..11386
                     /locus_tag="PLB01_02790"
     CDS             10904..11386
                     /locus_tag="PLB01_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76257.1"
                     /translation="MKVRKLADAHEKAARRVDELAARALTLEGFDDPADVSKLLKARA
                     EHAAACAERDALAVALEGAQRVERAEAEAQAARELEARQRRDRVAAEAVGSEITDALT
                     ALGRLLSKHEALVDALPGTERYRFWPPTYAETASKQLTAGVKLTRDIEVRLTVPVGSE
                     "
     gene            11434..12690
                     /locus_tag="PLB01_02795"
     CDS             11434..12690
                     /locus_tag="PLB01_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQQ76258.1"
                     /translation="MKPKRLPDGRWLIRYFEDGTKQGTYRQERLDARLTKAEADKAYK
                     QRLHEAASRRGKGYARQTFGELASRYVKAHGPKLSESWRSALEGMLRLHISTRPRRRA
                     DGEIVTNTAGEVVLDPVFRDRLIENIRPVDVEEYRNERREAGAKNATVNREVAVLLSI
                     LNFGARNDLIERNPIPHGRISKLPEVAKDLYFTPEEWQAFSGAFDDAKKWEAFRAKTR
                     GFGPVLMNPTKGTERRHGAGPKPEGETSHEYRERLRAAMDVFRAVLFTASRIGEVLTL
                     TWNAIDLPRNIVRIYQHKTKRTKTLTISPDLRAVLDARPRGIGQTLVFQRPGGGPWDP
                     SKLKGAFRLAKKISGVRPDLRIHDLRHTAASWMTLAGESERKVRDVLGHTDVRTTARY
                     AHLAPNDLQGAVNVIGQMARANGSTR"
     gene            complement(13074..14045)
                     /locus_tag="PLB01_02800"
     CDS             complement(13074..14045)
                     /locus_tag="PLB01_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HQQ76259.1"
                     /translation="MSGFIDRLKELDRFPNKELSVEADVLGRELIGPLPFTFRMDERK
                     LGDFDLELLLSRLEMQSDLDAIDARRKSGKPELSMASVLSTSPQRDAASSRHSIASYI
                     QMIYGSLPPNADEVLRRVHIKFLKRLHSLSGEPLEVFTTNYDPSIEKVGVLDPSLKVA
                     QGFEPNADEGVMVFSDQAFEGPEPIRLYHIHGTFHWQEDRGRIISVPVTHSRSLGKVL
                     LPGPRKAVDAALPPFKSLYARFFEKVREADRIISIGYSGRDEDVTYRLLLGMGANPDS
                     RLIVVMPGKPAFGWLTAKEILGDRFIHLPLKFEDLVDDPDFWRLVEN"
     gene            complement(14203..14279)
                     /locus_tag="PLB01_02805"
     tRNA            complement(14203..14279)
                     /locus_tag="PLB01_02805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14243..14245),aa:Pro,seq:ggg)
     gene            complement(14346..14606)
                     /locus_tag="PLB01_02810"
     CDS             complement(14346..14606)
                     /locus_tag="PLB01_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76260.1"
                     /translation="MRVAIARFNSSTFEVVHPDGKRRAFLKREDPEFVDALEAEAAYN
                     AKVAKMPFGLGRLVPPPSIEIPDARWERLRARISGSFPAVKL"
     gene            complement(14674..15387)
                     /locus_tag="PLB01_02815"
     CDS             complement(14674..15387)
                     /locus_tag="PLB01_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQQ76261.1"
                     /translation="MNELAVAAALTFAASAHCVGMCGGFAAAVSGGARRWKLVAGQLL
                     LQAGKATSYVFLGALAGALGGAIVKSAALSMGGRVVTVVAGLALLFAGLALLGLLGRG
                     EDALSRWLAPHWARVMRPLLEQRPSGFPLVVGMAMGFFPCPLVYAGVAAAAATGSPLR
                     GALTMAGVGLGTVPALLLSAAFGSMLSAVWRKNVARVAGVLLVVFAALTIARGFGVPV
                     MRVLHLGGSMPPAQEAPCH"
     gene            complement(15384..17495)
                     /locus_tag="PLB01_02820"
     CDS             complement(15384..17495)
                     /locus_tag="PLB01_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQQ76262.1"
                     /translation="MSAVGVAAASSQALPPLPLCAHCGLPVAGLFRLPSGPCYCCGGC
                     ALAHRLTGGASGHGQASGLLMAVGVGAFLAMNVMMCSFVLYTRGAETDRAAGEAWVRW
                     ALLVLATPALFLLGLPFLNRGLKRLKDRALDTDALIVIGVAAAFALSVRSVLSGGGPI
                     YLDTAMGILLFVTIGRYLEASSRARTTDALSALVRQLPSEAARVVNGIEEMVSVDALR
                     PGDVIRVRPGERIAVDGTIVEGTASVSEAEITGESVPASRGPGDAVAAGTLNQDGSLL
                     VEARRTGAETTVARLVKLVAESRAGRYTLAPLVDRLSAAFVPFVLLVATLTLAYWTRR
                     AGLGEGLMNALSVLLIACPCAIGIATPLASTAAVGRAASAGVLVRSGAVFEALARAKC
                     VFLDKTGTLTTGELSLAEVRPAAGFDRQGLLELAAALEHGSEHPVGRAICSAASAREK
                     EREEKISLKVEEFRAVAGRGVEGLVGGRRVRIGTAAFVGAPSGVADVEAPPGRTAAYV
                     SCSGTFAGILFFSDRTRREAAEAVSALRARGLSLEILSGDQPSRVTAFAADFPGLRAA
                     GGLSPEGKLDVIRSSVRAGESPVMVGDGINDAPALSVASAGVTLESGTDLAREVADVT
                     ILGGDLRKLPWTIELAHRTVRTAHLNLFWAFFYNGIGITLAVCGLLHPLFGAVAMIAS
                     SLLVVVHSQRLTRFPLPGASA"
     gene            complement(17492..17677)
                     /locus_tag="PLB01_02825"
     CDS             complement(17492..17677)
                     /locus_tag="PLB01_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76263.1"
                     /translation="MKTPKASFHPSEGRVLYALLWIVSFAAIAGLVWVWLGARKANPV
                     LLNLETGQPVATPAPRH"
     gene            complement(17677..18903)
                     /locus_tag="PLB01_02830"
     CDS             complement(17677..18903)
                     /locus_tag="PLB01_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ76264.1"
                     /translation="MALPGWTGRFAKQRRLIQLGFFVVFALLPLFDLFRFDFTISRFY
                     FFRNEIWLDEWTLIWLALMFAMWLVGAASLILGRVYCAYACPQMVFSEFANDLDALAK
                     RIARKISSNGRGAAVRAVSLALLAPVSVLFSVLFMAYFAPLPEVVRRLVRFDVGPWVG
                     LLGLLTAVLFFLDFAFLRERFCRSVCPYGLLQGILEDGRSLHVAYSEATGPCIQCNLC
                     AKVCPMEIDIRNGPYQIECTRCGNCIDACNLILGKKKRPGLLAFDFGSAALTQWDAKR
                     VLVAVSTVGFGVALVLAIVLRQDVSFRLSPVYTEQAAGPSEFAESRFLLRATNKGKAP
                     VTLAVTSEGLPKDVALDGMQDPSVPAGTEKRFTIIVRVPRAEVASSVVPFTLTVKAKD
                     RSETFEAALFVAGRKG"
     gene            complement(18906..20831)
                     /locus_tag="PLB01_02835"
     CDS             complement(18906..20831)
                     /locus_tag="PLB01_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ76265.1"
                     /translation="METAISSAYRRLRIPDAERYWVEQVKCRVGCPVFTDACGYVTAV
                     AEGRDEDAYAAARATNPFVSICGRICGAPCEAACRRGSVDAPVAIRAVKRWACELHGN
                     ETGSHEKYHRYADERMLVPKGNYAEKIAVIGSGVGGLTVAHDLARAGYPVTVFEANPV
                     PGGMLTLGVPLYRLPRELVKAEIDAILSLGVELRLNARVGAPGLTMSDLRAQGYKAFF
                     IGAGLQGSRRLKIPGADLPGVVNGLEFLKLVNLGEKIDIGPRVLVIGGGNVAFDVARS
                     ALRNDANETEDFRVTADVARTAARRAGVTEVHVACLESREEMPADAVEIEEGLHEGLH
                     LHTSLGPRAIQGNGKVSGVEFVSCLSVFDSEKRFNPKFDETMRETIEVDTVIFAIGQS
                     AEFNFLTPEDGIELTDRGLVKVDSETLQTSRPDVFAGGDVVLGPRLFIDAIASAQVAA
                     RSIHDYLRKSTTSVVLNYAWSPAAYTMGPDWEIEERRLPPSEAPRPNELPAAADRLVE
                     RNYPEHEARRQAERCLRCDVQTYFEGQHCIACNGCVDVCPDDCLHLVGLSQVASDPRL
                     LAMAGEQLGITKETLAAFEPAELDALGGVMLKDETACIRCSMCATRCPTRAFTMQRYA
                     FHRELQVEPVQNARLKY"
     gene            complement(20838..21149)
                     /locus_tag="PLB01_02840"
     CDS             complement(20838..21149)
                     /locus_tag="PLB01_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76266.1"
                     /translation="MADKPENEKIHELAGGWITERAGTPIPTFLKFTYVGFCLFGLWY
                     LYAYWAGETTHSTRGTLVQEANRIMSVPGAGWHAVIGVILLAFVASLLAFAFRSREDG
                     E"
     gene            complement(21142..21315)
                     /locus_tag="PLB01_02845"
     CDS             complement(21142..21315)
                     /locus_tag="PLB01_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein"
                     /protein_id="HQQ76267.1"
                     /translation="MTFAVWDPIFGVYALMASFVASALCLLLWAIRTGAMKDDEAPKF
                     RMLDDDDGGTKNG"
     gene            complement(21318..21884)
                     /locus_tag="PLB01_02850"
     CDS             complement(21318..21884)
                     /locus_tag="PLB01_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HQQ76268.1"
                     /translation="MSKVDKSSGLFIGAAVGFFAVGAILTTFAPPFFDASWTKPVPGL
                     KNYAVLAKSGDAEARRVVQGRAIYVREGCWYCHTQQTRTIEADTVRYGWRGVKSPVST
                     PDEFVYDAPHMFGTRRVGPDLSRVGGKYDRQWHVTHFRDPRALVPGSVMPPFPWIAND
                     AKELDALVAYVQSLGRQKDWRPAHDYEQ"
     gene            complement(21895..23295)
                     /locus_tag="PLB01_02855"
     CDS             complement(21895..23295)
                     /locus_tag="PLB01_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQQ76269.1"
                     /translation="MSSVAAAARPGSAAQGLEHEDRAAFGFILVASLFFVISGLVALI
                     VAGKLNMPTLLGNISWLQYGRMRPLHVNGMLFGWLLAADMGLSFYLIPRLCGVKLWSE
                     KLGMATLYLWVAIVLGAVVTLAGGVTQGLEYAELPLVLDVLVTIAWVMFGLNIVMTVV
                     TRKYQQMYVAIWYIVGGILWTAVVYIVGNFATMFTSGTNQANLNWFYVHNAVGLIFTP
                     LGLGIGYYFIPKASESPLYSHRLSMVGFWSLAFVYVWTGAHHMLHGPISQWLQTVAIL
                     FSVMLMIPVWSLVWNFLATMKGQWHQLKDNVPIKFLMTGTIFYLLTCFQGPMHSLRSV
                     NAIVSKTDWIVGHAHMAVLGAFSFFAIGAAYYAIPRMLKTSLHSDKLANATFWLWLIG
                     GLSFFVSLWIGGFLQGLQWNDVTIPFIDTVKFMRPYWLVRLVGGTLMFTGILIFFWNL
                     LMTWTGRRDRAAVAAA"
     gene            complement(23300..25066)
                     /locus_tag="PLB01_02860"
     CDS             complement(23300..25066)
                     /locus_tag="PLB01_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQQ76270.1"
                     /translation="MRAALAFISVVVLALHAYVFNNQFFARWQDYQKKYFKDAAAAST
                     NDAVRATLVARRPAIEQTIVRSFGSERIDRCQTCHIAADDPRFTKADQPLRTHPPVPG
                     HRFETFGCTICHDGQGRAVDAEQAHGTEEWPWPVLPKTLIQSNCVQCHTQSDWPGAPL
                     VNAGRRLFFERACYTCHTIAGLSYGSIGPELSEVGRRRRWEFVHAKIENPRWNNPTST
                     MPRQDLTNEQRMSLVAFLKAQQGAHISRAPLAKFQAAQAERPKWLPLGLIVGHDAEVA
                     FAAQAPAQRGEALLQRVGCLSCHKLGERDGRVGPELAFTSAQRDDKWMLAHFRDPKSV
                     VPGSLMPPYPLPDDIFDALSAYLLSRRPPEIPAEPEKRYAALCSRCHGEKGQGNGLIA
                     EYLDPKPRDLTKAAFMRTKTRERLVASLVNGVPGTSMAPWGRILGGEAQAGALVDYVL
                     STWSKDSRAQVPKGRVIPAANPVAYSKESVERGEAIFLNRCWGCHGKKADGHGPNAED
                     IVPRPRNLRNAPFVASVSYTRLHESIKYGVQGTAMPAAGFDFSLDDKAIGDVVNYILS
                     LTRPAPKGEPAKVAEFENTAGR"
     gene            complement(25070..26440)
                     /locus_tag="PLB01_02865"
     CDS             complement(25070..26440)
                     /locus_tag="PLB01_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQQ76271.1"
                     /translation="MKSEQGFLGTLRSLPANVWNSIFRNPLPSSDLGRSQTTFTNFFL
                     HIHPVKTHTNSLRATYTLGLGVMSFSLFVILLVTGILLMFYYVPSVEQAHNRMLDLSG
                     TVAFGVLLRNMHRWAAHGMVAIVLLHLCRVFFTGSYKKPREFNWVVGVVLLLLTLFAS
                     FTGYLLPWDQLAFWAITVGTAIAGYAPVVGEQVRFLLLGDRVVGQEALIRFYVLHVAV
                     LPLLMTVLIGVHFWRIRKDGGLSRPEGSDLPEAGEKNALALQAKLEGLASKAKLAKNK
                     TFGVMGIVRGPFPKVGNVPDNTVSSWPYLFIAELFVFAVTLLVILVLAVVLNAPLEAP
                     ADPTHPPNPSKAPWYFLGLQEMVSYSAFWGGVGIPTIFVVLLLLLPYLDRGTKGVGVW
                     FSRHRLLATTTFTTLLLVNLVLVVVGTFFRGPNWAFVSPFDKPAVSEHAPPPPAPAPS
                     TAPAGR"
     gene            complement(26451..26927)
                     /locus_tag="PLB01_02870"
     CDS             complement(26451..26927)
                     /locus_tag="PLB01_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQQ76272.1"
                     /translation="MTKPTEAKPSMTMSRRRALTITSVVSLFGANAVAVFSNLLFFKP
                     RVTYGEPSRFRIGKPEAYPPGTRLPLTRQRVTIVRTEAGMCAISNTCTHLGCVIATTE
                     IGFDCPCHGSRFDAEGTVLGGPAPRPLPWFRIALSPNGELEVDTAEAVPAGTFLKV"
     gene            complement(26924..27118)
                     /gene="ccoS"
                     /locus_tag="PLB01_02875"
     CDS             complement(26924..27118)
                     /gene="ccoS"
                     /locus_tag="PLB01_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQQ76273.1"
                     /translation="MTITVMLFAAGIFMGIAGALAWAWGVRSGQFRDLEKTKEQLFWP
                     DLAEDGGDGAGPHTEPRRRT"
     gene            complement(27264..27803)
                     /locus_tag="PLB01_02880"
     CDS             complement(27264..27803)
                     /locus_tag="PLB01_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQQ76274.1"
                     /translation="MAEVNGSGGETRYKIGDVCRIADVQPYVLRYWESEFPVLAPDKS
                     IPGPRTYSARELKLIEQIKRLLYDEGYTIAGAKKRLESESGGRAESKDAGADTNPAIV
                     PIPSAPKPPKVEKPRPPRPPKAAVPAITDASSELPPPEVTQPVDVPAAAPGAPVDDRV
                     PRAVAELKQILALLSRSEP"
     gene            complement(27866..29227)
                     /gene="der"
                     /locus_tag="PLB01_02885"
     CDS             complement(27866..29227)
                     /gene="der"
                     /locus_tag="PLB01_02885"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQQ76275.1"
                     /translation="MAAFKVVLVGRPNVGKSALFNRLAGERRALVHDQPGMTRDALAV
                     VAKTPSGRAYELVDTGGLDLDAAGGFAAWTSDRTLSTVADADLLIFVLDGVAGRLPED
                     ERIAQRLHALGKPVLVAWNKVDAKSAADSTADGYQLGFPQVVGVSALHGTGADEIFDA
                     IEAAVPPDLDPDTRTEPLPIAIVGRPNVGKSSIVNALLGRDRVMVSEIAGTTRDAIDC
                     RLSVGGREYVLVDTAGIRKKGRTTDAPEVLSVVAARKSMERARLVVIVFDAGDGITAQ
                     DAHIAGYAEEAGRGLLLVANKWDLVEKDHEKVREMKQDVVRRFVFLRNAPFLPISAKT
                     GKGIGRILPEADALAKRFATRMSTGELNRVLKKAFERELPKGRSGRELRIRYAVQVAS
                     EPPLVRLFADRAEALHFSFERFLQNRIREHWKLDGVPVKFIVKPGDEKNRDGRKNEER
                     RTS"
BASE COUNT         4813 a         9922 c        10204 g         4421 t
ORIGIN      
        1 cccgcgcgtg acacgcggcg attgcggcct gcagcgcgta cggcccgcgg ccgccgagcg
       61 cctcggcgcg cgcgagagcc gcgagcccgc gcccgatgag gaggtagtcc catcggccgc
      121 ggtcctggtc gaggagcagc acgggctcgc ccccggaccc gagccgcgcc ttcgcccggg
      181 aggcctggat ctccatgagc gcgacgaggc cgtggacttc gggctcggcc ggcgcgagct
      241 ccgcgaggac gcgtccgagc cgaagggcct cctcgcagag ctgcgggcgc atccagtcgt
      301 cgcccgcggt cgcggcgtag ccctcgttga agatcaggta caggacctcg aggaccgagg
      361 agaggcgcgc gtcgcggtcc ggaccgcgcg gcacctcgaa cggaacgcgg gcgtccgcga
      421 gcgtccgctt cgcgcgcacg atgcgttggg cgatcgtggt ctccggcacg aggtaggccc
      481 tcgcgatttc cgccgtggtg agcccgccca ggagtcgcaa cgtcagcgcg acacgcgcct
      541 cgcgggagag gacgggatgg caggacgtga agacgaggct gaggaggtcg tcgccgatgt
      601 cgtcgtccag cgccgcgtcg aggtcggcca ccgccctctc gcgctccgcc tcgaggtcgc
      661 ggccgatctc cccgtgcgcg cgctcgacgc gccgtccgcg gcggacgtgg tcgatcgccc
      721 tgttccgggc cacggtcgtg agccacgcgc ccggattgtc cggcacgccc gcctcgggcc
      781 atcgttcgag cgcggcgacg agcgcgtcct gcgcgaagtc ctccgcgagc ccgacgtcac
      841 ccgtgacgcg cacgagcgtc gcgatgagcc tcggggactc gatacgccag atcgcgtcga
      901 tcgcgcgatg ggactcggga tccgccacgg gggacgatta gagcatcgcc ccccggcggg
      961 cccgtcacgt cttcgaccgc tccgcgaact tctcccgacg ctccttcatc ttctccagct
     1021 cgggcgtgag ctcgccccag tccgagacgt cgaggacctt ccggatctcg atctccgact
     1081 cgccttcatg agggttcggg cagcgcttca cccactcgat cgcctcctcc atcgacttca
     1141 cctcccacag ccagaagccg gcgaccagct ccttcgtctc cgtgaacggc ccgtcgatca
     1201 cggtgcgctg gctccccgag aaccggacgc gcaccccctt cgagctctgc tgaagtccct
     1261 cggcggcgag catgacgccc gctttcacga gctcctcgtt gaacttcccc atgtcggcga
     1321 gcagcttctc gctcggcatc acgcccgcct cgctctcctt cgtggccttg accatcacca
     1381 agaatcgcat ttcgtcgtct cctttcggcc ggattcccgg ccctcgaccc cacgacgaac
     1441 gagccccccc gaaatcgaca cgtccgggcg agaaatgttc tcagccgcca cgagcctagc
     1501 agggccgttc ccggggcgga ggtcgagggt tttcttgctc cgggcctgcg ggtatcgttg
     1561 gccgatgagt acgaaccgaa acgttttcct gacggctctc ctcgccgcgc tcgcggccac
     1621 gccgctcccc gccccggcgg agtcggcgtg cgaagccgtc ggtgccgccg acttcggcgc
     1681 actcggcttc ggcacgatgg gcgccgggag cggccgcaag ccggcctatg tcgtcaagct
     1741 ggactcgaac gccgcgatct ccggtttgga gaccgagtgc ctgtggaatt ccggcccggg
     1801 cagtctgacg ctccttctcg gcgacatgga cgcgaacctc gtcaagggcg acgacgtccg
     1861 ccggctcggc cagaaggtgc tcgccgccgt gcgcgagttc cacgagacgg ccggaaagta
     1921 ctccggccgg cccatgacca tcgcgcccct gccgggcgtc ggcgacgagg cgtactcggc
     1981 gcggctcgac gcccacttct tcgaggtctg ggggcgcagg ggtctgcggc tcgcccgtct
     2041 caagatctcg gcaccgccct cgatgaagcg cagcccgacg accgagcagg ccggtgcgct
     2101 cctcggacga atcctctcgc atggaagcgg ctacacgacg tccggggcgc ccgcgatcca
     2161 gggcgagtcc ccttggtccg gacccggcga caaatacgtc gtgacgggct ccatcgtggc
     2221 gacgggggcg ctgagcggca ccttcacatg gtcgagcccc ggcgaggtcg agacgtaccc
     2281 cgatcggacc gagatcgttc tcgcgaccgc cgacaagtcg gcctacatga acctgcaggt
     2341 gtggaacggc gaggatcggg tcgaggtccg ctcgggaaag ctccgttctg gattcctccg
     2401 cgggtccggc cgtgtggcga aggtggaccc acttgcgggt tccggcgaga tccgcgtgga
     2461 cgcgacggtc aagtcggcga cggagacgat caccgtcaag ggcgcgctca cggtgaggcc
     2521 cgcgaggaag aagtgacgcg cctcgctccc gcggagctgc cgtgaccggc tccgggcggg
     2581 aacggggcgg gcccgtcggc ctcgggtgcg ggctgctgct cgggctcgcc gtgggcgccg
     2641 cgtgggtcgt cctcgacgga ttcccgccgt ggacgttcgt ggttccggcc gtgtccctcg
     2701 cggcggtcgc gtatttctac ggcgaccgct tctgggagtg gctcgccgag cacggctgga
     2761 aggtgccctg gttctagggc cgcaggatta cttcagccgc ggcgccagcc agtcgaccca
     2821 gcggcggaag acctcgcgcg tgcgcacggg gtctcccggg aaatggccct cgaccgggat
     2881 cgcgacgaac gacgtctcga cgccggcccc cttcagcgcc cggaacagct tgaacgcctg
     2941 cgtgtatggg acgcggaagt cgcccgtgtc cgacaggatg agcgtcggcg tcttcgcgcg
     3001 cgtcgcgtac gtgatcggcg actgcgcgcg gtagagcgcg tctccgccgg gcgagaacgg
     3061 cgtcgcgttc cccttcatcg cgtaccgcat ctggacgttg aagtcggcga acgcgtactg
     3121 gtccgcgagg tccgtcacgg gagcccccgc gaccgccgcc ttccagcgcg tgtcgtgtcc
     3181 gatgagccag ctcgtcatgt agccgccgta cgaccacccc gagaccgcga tccgcgacgc
     3241 gtcgacgaag cccttcttca cgagggcgtc cacgcccgcg aggacgtcgc ggcccgggcc
     3301 gtcgcccgcg tcgttgaaga tcgcgcgctg gtaggcgttc ccgcggttgt cgctcccgcg
     3361 gtagttcggc atgaacaccg cccagccctg cgcggcgaag agatgggccg gggccgggaa
     3421 cgcgcgcgtc gaggcggcct gcgggccccc gtggatgtag aggacgagcg ggaccttcgc
     3481 gcccggcgcg aggcccggcg gcaggacgac gacgccgtcc tcgtcgaaac cgtccggccc
     3541 cttccacgtg aacgtctcga ccttcccgaa atcgcgcccc gcgatctcgg cgttcaggtc
     3601 ggtgaggcgc ttcggagcgg acgaagggga cgccatccac cagagctccc cggggcgatc
     3661 cggcaggctg cccacgaacg cgatcgcccc gttcgggccc gccgacacgt cgacgaagaa
     3721 cgaccacgcc gggttgacgt ccccgagctc gagcctcttc gcggggccat caaggggtgc
     3781 cagccagagg gagacacgcg ttccgtcgtt gcctcccgtg agcagagcct tgccgtcggg
     3841 cgtccatacg gacgcgtaca ggcagcggtc gatcggcttc gtgaggttca cgcccgcgcc
     3901 gccggacgcg ggggcgacgt gcacgtcgtt gacgttgttc gggtcgcggt cgcgcgggca
     3961 ccagtacgag atcttcgagc cgtcaggcga gaagaccggg aagccctcga agtgcgtctt
     4021 gcccgtcagg gcgcggatcg agccggactc gacgtcgagg acccgcaccg tcgccgcgtc
     4081 gttgtcgccc gagtgcggcg tctcgacgcg gacgaacgcg agggacttgc cgtccggcga
     4141 ccacgagagc ggcgaagacg gcgggctcgg cggcagggcc gcggggaggc tccacgcgcc
     4201 ggacgtgagg cgtttcgcct cgccgccctc ggccgggacg agccagacgt gaaccggcat
     4261 ggcggcctcg gagacgtaga ggctgtcgct cccgacctcg aacaggtcgt tgcccttctc
     4321 gatctcggcc ttgttgggcc gctcgtcctc ggcggcaaag gcgaaagcct tgccgtcggg
     4381 ccgccacgcg aagtgctgga cgcccgtcgg cgcgttcgtg accctctgcg cctcgccgcc
     4441 tcccatcggg agcacgaaga tctgggactt cgcgtccttc cccgcgccgt cggccgcgag
     4501 gaacgcgagc cgttcgccgg acggagacca gcgcgggtgc gagacgccct tgcggtcgtg
     4561 tgtgaggggc cgcaacgcgc ccgtcgagac gtccacgagg ctcagctcgg acagccactt
     4621 gttcttcgcg acgtccgcgc gcgtcacgac tacggcgatc gacttgccgt cgggcgaaat
     4681 ccgcggcgac gacaggcgga cgatcttctc gaagtcgtcg accgagaagc gcttctttgc
     4741 cgccgcttcg tccgcggcga gcgggagcgc gacggcgaga agcaggcagg cggcgagcgt
     4801 ggagaggcgg cgtgcgatgg tcacggatcc cccttcgacg acggacggtg tgtcgggaat
     4861 cctacgcgcc ccgcggggga aggttcagtg cccggatcag ccggcggggc ggtcgaacga
     4921 cgtcccacgg gacgggcggg tccgctgcac ggcctcccgt cgccccagct cctcacggac
     4981 ctcccggacg aactgcgcct ctcgcgtcgc gcgcatccgc gcccgggccg cggcctgcga
     5041 cgcctcgatg agggccatgc actgagggca cttcgaaggc gtcagccagg ccccgccgat
     5101 tccgtgaacg cacggagtcg cgtgcagcgc caccgatatc tcgcgctccc gggcggcgat
     5161 cccgtcggga tctccccggc tcaggaatgc gaagacgagg cggcccatga actcgattcg
     5221 cgtgccacga acatagtccc ggcccggccg gcgagccact ccggttgatt ttctggcttg
     5281 aggaggcgag atggtcggga cggtgggatt cgaacccacg accccttgaa ccccatggaa
     5341 atccgcggcc ttttcggcct tcagcagaaa cactcgaaaa ctggcgcaag tagctctccc
     5401 cgttgagctt cgactcccct gcgctgtgac accgtgtgac gccaaaagac acgaaaagac
     5461 caacagggtg acgcgaaagt gacgcgcgat cttgtctcaa ctgagtgcgg acgcccggtg
     5521 accgagaggg acgacgcgcg aaagcgataa cgcactaggc gtcggagtcc tcgtcctcgt
     5581 cgtccggcgc gtacctcggg cagtcgcacc cggccacgag gcattcgagg ccgtacgacc
     5641 cgacgaggcc gccgacgtcg gagatcgtcc cgcggtgctc gtcgcgcgcg tggccgcacg
     5701 tgcaaggggt cttctcgtcg ccgatcttca tggcggctag aggcccttcc attgccagcg
     5761 ataaatgact ccgtcactgt tcgaccagaa catgcggtac gcggtgtacc ccgccgcgtg
     5821 ctccggcgtg taagtcgtgt agctctgtcc gttcgcgacg aacatatttc ccacggccat
     5881 gccgttcacg taggttgttg tatgtgagga cccggctacg gtgtacgtgc ggtccaagag
     5941 gtagacgagc acgcgcccac cctgtccgtc gtcgtagacc tgttgaggcg gcccccaact
     6001 cgtgacgaga ttggagacgc tgtccccgac ccacgaggcc ataacctcgt tgaccgaagc
     6061 gcacccactc gtgcagagga caagacacag gacggtcagc gccgcggctt tcctcatcgc
     6121 gccctcctat ccgggactta gatttgagac atattctatc gagtcggcgg agatgggcct
     6181 cgacggtcac tcgtcggcac cttcgcgaat acaccgaacc cggtcggacg tcgacacccc
     6241 ggcgtcgggc acgcgcggaa gccgggcgat cccttccgga cgtgaacgat ccggtggcag
     6301 tcgcggcaga agtagttgtc gaagtccggg aggttcacgc cgataggctc agaaggcgtc
     6361 gcgagcgtgc cggtcgacat gggacggcga ggatagacct cgcgccggac cgggcgacga
     6421 tcccgtttaa acgcgagatg gcgtgtcgag agacgcgaag gcagcgggaa taatctagtt
     6481 cgcccgcgct tgacggcgcc atgcgttgcc gggtaaaacg cggacgtcaa ctcccgcggc
     6541 ggggcaacgc gggattcccc atcgaacgcc ccagaagtgc gccgcgtacg gcggtggaag
     6601 tcttctggga ggtttcgatg aaccagccac ccgccgcgcc ccttctcctc gacgagaacg
     6661 aagccgagcg gtactccgga atctccaagt acacgctccg cggctatcgg cgccagcgcc
     6721 gcggtccgat cgtcgtgaag ctcggtcgaa gcgtgcgtta ccggcctgag gatctttccg
     6781 cgttcatcgc ggagaacgta atcccgcccg gtgacatgcc gaaggtcagg cggaggccgt
     6841 gccggtgagc gtcccgcctg atcacgctgc ggcgcgcgtc gctctcgacg acggcgagcg
     6901 gctcgctcgc atcctcgaat ttgcgcggac cgataccggc gcgctcttgc gcgacgctga
     6961 ctcgcggcgt gctgcgcggt caccggagga gcgccgcgcc gccgctgagg ccgcgcttcg
     7021 gactcgcagg caggcggcgc gcgcggtcgt cggcgatcgt gctcccgagt ggctcgcgga
     7081 actcgtcgcg caccgcggca cggagtccgt cttcggcgga ccggacggcg cactgctcaa
     7141 gctcgcgaac ggcatcgcct tcggcgagga gcgcacagtc gccctgcggc aactcgcgga
     7201 ctatctgctc gcctgcggcg tctcggcccc tatggcgctg acctttgtcg gagcgtgggc
     7261 gaacacgttc gccgccccgg tccctaacga acaggagatt ctccaagccg tcgcgcgcgt
     7321 cgtcgagcag cggcagcgga cccggaggcg tgcgtgaggg acgcgctgga agtaatcgca
     7381 ggctccgagt acggccagct gatcgcgccc ccggacgcgg atgcgccgct ctccgtcgtc
     7441 gacctcctcc ggcgggtcta cgtcggcgat cggttcccga cgggcctcgc ggcactcgac
     7501 gaacgcctca agggcggcct cgcgccggga ttcctattcg tcatcggcgg ggagccgtgg
     7561 atcgggaaga cgactctcct cgtgcagatc gccgacgcgc tcgccgcgga cggtgtcgcc
     7621 gtcttcatct gggccgccga cgagccgccg gagggcctcg cgctgcgtct cgggcaactt
     7681 cacggcgagg agcagcacga gctgaacccg aattacccgc ccgtcctcgc gcggctcgcg
     7741 ggggaactct caaacgggcc gcggttcctg ccggagtccg tcgagacggt cgagcaggcg
     7801 gcggactttg tcgagcgaca cacgccagac ggacgggtcg gtgccctcct gctcgactcg
     7861 cttcagtcgc agtccgagcg cagcggcgac gatgcggact ctccgcgcgc cgccctctcg
     7921 ggcctcatgg ctgagcttgt cgaggcgaaa aagcgcggcc tcatcgtcgg cgctgtgtcg
     7981 gagctggctc gcggcgccta cgcctcaccg gatcccggcc agcggacgcg cgggctcgcg
     8041 gctttcgccg aatcgagggc catcgagtac cgctccgaca tcgcgatgat catgacgtcc
     8101 gacggcggcg gcaacgtgcg ccttgaagtc gtgaaaaacc ggctcgggca cctcaagggc
     8161 ggcctaacgc tgaagctcaa ccacgcacgc gcccggttcg gggcgatcgc tgacgaggtg
     8221 ctcaagcgcc aagccgacga cgaacggcag cgggtagagc aggagcggct cagagcggac
     8281 gaggaccgcg tggccggcgt tctcgaaaag gccgagcgcg gccttacaaa ggacgagatc
     8341 cgggaccgcg cccggatgaa aaagcaggcc gtcggcgagg ccctcgtgcg tatggaggac
     8401 gaccgaccgc cgcgggtgag gcgtttcaag ccgccacgcg aaccgaaaca gtcggggcct
     8461 gttccggaac gttttgaact cccgcgaggg ctgggcgtat gaggaatttc ggcactcgtt
     8521 ccgactcgtt cccactcgtt ccgccactcg ttcccgaacg agacggcggc aactcgttcc
     8581 cgttcccctc ccttagggag ggaacgagtg ccgggaccga gtggacagaa aagaaagacc
     8641 actactcgtt cccgagaaat atgcgccctg tcggaggcgt ccgatgagcc gccggtgcga
     8701 gggatgtcgg cacccggagg cggcgcgaat cgcgtccctg ctcgcttccg gagagtcctt
     8761 tcgggacgtt gcgaaacagg tcggcctctc ggccagcgcc atccaccggc accgcgcgca
     8821 cgccgaaatt cggagcgcga tcgtccgtgc cgacgaaatt ctgaaagccg tcccggtcgg
     8881 cccggctcac accgacgtcc gcgagtacat ggccgacgcc cggcggctgg ccgagaaggc
     8941 cgagagcgag cgcgattaca ggggcgctct ggccgcggtc aagatcctga ccgacgtggc
     9001 cctgcggctt gaggagcggt cctccggcca gcccggaggg ctagtcacga tcgaattcga
     9061 cttctcggcc atgcagccgc accgcctggc tgcgcccgcc acggagccgc ctgcgggcca
     9121 gtccgatgag ggcagcgatg agtgaagcga ggctacttcg aggccgtcag cttcggcgag
     9181 acgcggcgca cgatgccaaa cacgacggac ggatcccagt tggtgcccgt gcgcgtcggg
     9241 atgccctcgg cgttcagcgt ctcggcgatc ttggcgaagc tcatccgacc gtgggcgcgg
     9301 ttgccgcgcc tgagcgtcag gatgcggtgg agaaccgcgt cctcgcccgg ctgagacccg
     9361 aacggcttgc gtccctcaca gcggccctcg gcgttgcgct tggcctcacg ggcggccctg
     9421 agtttgcgga cgaggagctt cttgtcgagt tgcgcgaaca ggccctgcat cccgatgatt
     9481 gcctcgcgca tcgggtcgtc ggtggtcgtg acgtcttcgc ccgtgttcgc ggcgatcagg
     9541 gcaacacccc gtttgcgaag ctcgccgagg agcgcgatct gcacgcccat gtcacgggcg
     9601 agccggtcga gcgactcgac cacgatcgtg cgaacgccgt tcgagaggat cgcggcgagc
     9661 atctcgacga aagcgggacg gtcggcctcg gttccggtgt gcgcgtcgcg gtaaacggca
     9721 gcgatctcga ccttggccgc tttggcgtag gcccggatcg cctcttcctg ccgaccgaac
     9781 ccgtgaccgc caacctgacc acggctggaa accctgaggt aagcgtaagc ctttttgctt
     9841 gccatgcatt gaatataaag gccgcaaccg ctcttgtcaa tggtaattct gtagaatcac
     9901 aagtaacccc ggaggccgta tgacgcctta caccaaacgc ccaccgagta accccgacct
     9961 gcatgacgct tggatcgaag ccgagcgtga tcactaccgc cgcattgaga gccgcacggc
    10021 tgtcaccctg cttgaacgcc gcttgaagcg tgcgggccaa catgtagccg ccgaggtact
    10081 ccggttcctc gccttcaccg gcggcgcggg cttgggctgc tatacggtcg gggcgctcct
    10141 cggcacggta cgcgacgcgc ttaacccgat cgtcttcggc gggaggctca acgcggaaga
    10201 gctggccgct ctcgggcggt tcaaccgggg gatcgccgag tgggcaagcg atgtgcccgc
    10261 gccccccccc ggtcgcggga aatctgtgca gctcgacagt ccgcgcgcgg cccctctcac
    10321 acccggacga agcccagaca cacgcataaa aacagaggcc ccatgaaaga ttttcggaag
    10381 ccggtttcgg ctccggcctc cattcagccc ggacgctccg gttcctcccg gcgggattca
    10441 tcgccccgcc gccccgtggc gtccgtcttt ttcaaccaca accacggcca gagggccaca
    10501 caggagaaat catgatcgct cccgacacca ccgatgccca gtcgttcgcc tcgaatctga
    10561 aactgcttac cgacctcctg cccgcgatgc acgaacccgc gcgacgtcgc gccgcgtggc
    10621 gcgtggctcg cgggagggcg gatctcgccg ccgccgtggc tgcggaaggc taccgactcg
    10681 acggagcgac ggcagcaccg tcgcccaaag ccgcaccgtc aaccgccgac gcgatcgccg
    10741 tcctccgcca tgctcaggcg gtcgctcaga acgcgggcgc tcccccggct gacgtcgcag
    10801 ccatccgaaa cgccgcgtcc gggcggatcc acgccgcggc gctcgaactc cagaagcgcg
    10861 atccgcgtct cagctacgag gccgcggttg ttgaggtgac gaaatgaagg tccgcaagct
    10921 ggccgacgcg cacgagaaag ccgcccgccg cgtggacgag ctggccgcac gcgctctcac
    10981 gctggagggg ttcgacgatc ccgccgacgt ctcgaagctg ctcaaggcgc gtgcggaaca
    11041 cgccgccgcc tgtgccgaac gggatgccct cgcggtggcg ctggagggcg ctcagcgagt
    11101 cgaacgtgcg gaggccgaag ctcaggccgc ccgagagctg gaggctcgac agaggcgcga
    11161 cagggtcgcc gccgaagcgg tcggcagcga aatcaccgac gcgctgactg cgctcggacg
    11221 gctgctctcg aagcacgaag cactcgttga cgcgctcccg ggaaccgagc gttatcggtt
    11281 ctggccgccc acctacgcgg agacggcctc gaagcagctc accgcgggag tgaagctgac
    11341 gcgcgacatt gaggtgcggc tcacggtgcc ggtggggtcg gagtagcacg cgataatgtt
    11401 cgccgacacg ctaaccggcc aaggaggacc gccatgaagc cgaagagact ccccgatggc
    11461 cgctggctca tccgctactt cgaggacggc acgaaacagg gcacgtatcg gcaggagcgg
    11521 ctcgacgcgc gactgacgaa ggcggaggcg gacaaggcct acaagcagcg gctgcacgag
    11581 gctgcgtcgc gtcgcgggaa gggctacgcg cgccagacct tcggcgagct ggccagccgt
    11641 tacgtgaagg cccacgggcc gaagctctcc gagtcgtggc ggagcgcact tgaggggatg
    11701 ctccgccttc atatctcgac gcgcccacgc agacgagccg acggtgaaat cgtcactaat
    11761 accgccggtg aggtcgtcct tgatcctgta ttccgtgacc ggctcatcga gaacatccgc
    11821 cctgtagacg tcgaggagta ccggaacgag cggcgggagg cgggcgcgaa gaacgcgacc
    11881 gtaaaccgcg aggttgccgt gctcctctcc atcctcaact tcggcgcgcg aaacgacctc
    11941 attgagcgga acccgatccc acacggtcgc atctcgaagc tgcccgaggt cgcaaaggat
    12001 ctctatttca ccccggagga gtggcaggcg ttctcgggcg cgttcgacga cgcgaagaaa
    12061 tgggaagcct tccgcgcgaa gacgcgcggc ttcggcccgg ttctcatgaa tcccacgaag
    12121 ggcacggagc gccgccacgg tgccggtccg aagcccgagg gcgagacctc gcacgagtat
    12181 cgcgagcgcc tccgcgcggc gatggacgtc tttcgcgcgg tgcttttcac cgcctccaga
    12241 atcggcgagg ttctcacgct cacgtggaac gccatcgacc tcccccgcaa tatcgtccgc
    12301 atctaccagc acaaaacgaa gaggacgaag acgctgacga tttcgcccga tctgcgcgcc
    12361 gtgctcgacg cgcgcccgcg tggcatcggt cagacgctcg ttttccagcg tcccggcggc
    12421 ggcccgtggg acccgtcgaa actcaaaggt gccttccggc tagcgaagaa gatttccggg
    12481 gtccgccccg atctgcgcat tcacgacctc cgccacacgg cggcctcgtg gatgactctc
    12541 gcgggcgagt cggagcggaa ggtccgcgac gttctcggtc acacggacgt tcggacgacg
    12601 gcgcgttacg cacacctcgc gccgaacgat ctacagggcg ccgtcaacgt aatcggccag
    12661 atggcgcggg cgaatggctc cacgcgatga gcgagtgtcc gttttgtccg gcgactatcc
    12721 ccgcggggca ggcatcgtgc ccgcgttgcc acctggcact cgggcgatgc gagtcgtgcg
    12781 gctcggatgg agcggtcgcc cttgtcgtcc tgaaggaaaa cgtgtcttac atcgttgagc
    12841 gtcgagagcg aaccttcgag gggttcgcct gccgccgttg cctgaaccga ctctttcgtg
    12901 agttcgaagg ccgcacactc gtcggcacat ggtggggtgg aatcggattc gttaagggtc
    12961 cggtcttcct cgtcctcaac gtcttcgagt atcttcccgc cttcttccgc ttcactcggc
    13021 tgcgtcgccg agtgggcccc gcccgtttcg ggctctctta gaagcgcaca gctctaattc
    13081 tctacgagac gccagaagtc gggatcatcg acaaggtctt cgaatttcag cggcaagtgg
    13141 atgaaccgat cgcccagaat ctccttagcc gtgagccaac cgaacgcggg tttaccgggc
    13201 atcacgacga tgagacgcga gtcgggattc gcccccatcc cgagaagaag gcgataggtc
    13261 acatcctcgt cgcgcccgct atagccgatt gagatgatcc ggtccgcctc gcgtaccttt
    13321 tcgaagaatc tcgcgtagag gctcttgaag ggaggaagcg cggcgtcgac agccttgcgc
    13381 ggccccggaa gcagaacctt accgagactc ctcgaatgtg tgaccgggac cgaaatgatc
    13441 cggcctcgat cctcctgcca atgaaaagta ccgtggatgt gatagagcct gatcggctca
    13501 ggtccttcga acgcctgatc cgagaacacc atgacaccct cgtcggcgtt gggctcgaaa
    13561 ccttgcgcga ccttgaggga agggtcgagg acgccgacct tctcgatgga tggatcgtag
    13621 ttggtggtaa atacctccaa cggttctccg gagagcgaat gcagtcgctt gaggaacttt
    13681 atgtgcaccc gacgaagaac ctcgtcggcg ttcggcggca gactgccata gatcatctgg
    13741 atgtagctgg cgatgctgtg ccgggatgaa gccgcgtccc gttgcggact cgtagacagt
    13801 acgctcgcca ttgagagctc aggcttcccg gacttgcgcc gggcgtcaat cgcatcgagg
    13861 tcgctctgca tttccagtcg cgagaggaga agttctaggt cgaagtcgcc gagcttccgc
    13921 tcatccattc gaaaagtgaa tggaagtggg ccaatcaact ctcgaccgag aacgtccgcc
    13981 tcaacggaga gttccttgtt cgggaaccga tctagctcct tgagtcgatc gatgaagccc
    14041 gacatagtcg ggtacgtgag gcacagagac gcccctgcgc cggtcaagac gacaaccttc
    14101 aggaccgtcc tcctcgccgc accagcgaca gggtgacgcg ggggtgacgc ggagccgcgt
    14161 gggcgtcggt catgaagtgg tcataacctc tttgtaatca gatggtcggg acggtgggat
    14221 tcgaacccac gaccccttga accccattca agtacgctac caggctgcgc cacgtcccga
    14281 ccgaggactc gcgtcgaagg gcgcgaagcc cttcgggggt agcggtttat accacggccc
    14341 cgatgtcaga gcttgacggc cgggaacgaa cccgagatgc gggcgcgcag tctctcccac
    14401 cgagcatccg ggatctcgat cgagggcggc ggcaccagcc ggccgaggcc gaacggcatc
    14461 ttcgcgacct tcgcgttgta cgcggcctcg gcctccagcg cgtcgacgaa ctccggatcc
    14521 tcgcgcttca ggaacgcccg gcgcttgccg tccgggtgga cgacttcgaa ggtcgaggag
    14581 ttgaacctgg cgatggcaac cctcactccg gcacctctaa cccgctcgat cgaagggaga
    14641 tgaagtaatt ctagacgagc ggctcacgga gcttcaatgg cacggcgctt cctgcgccgg
    14701 gggcatcgac cctcccaggt gcaggacccg catgacgggg acgccgaagc cgcgcgcgat
    14761 cgtgagcgcc gcgaagacga cgaggaggac gcccgcgacc cgcgcgacgt tcttccgcca
    14821 caccgccgag agcatcgacc cgaacgcggc cgagaggagc agcgcgggca ccgtcccgag
    14881 ccccacgccc gccatcgtga gcgcgcccct gagcgggctg ccggtcgccg ccgcggcggc
    14941 gacgcccgcg tagacgagcg ggcacgggaa gaagcccatc gccattccga cgacgagcgg
    15001 gaatcccgac gggcgctgct cgagcagcgg gcgcatcacg cgcgcccagt gcggcgcgag
    15061 ccaccgcgac agcgcgtctt cgccgcgccc gaggaggccg aggagcgcga ggcccgcgaa
    15121 caggagcgcg aggccggcga ccaccgtcac cacgcggccg cccatcgaga gcgccgcgct
    15181 cttcacgatc gcgccgccga gggcgcccgc gagcgcgccg aggaacacgt acgacgtggc
    15241 cttccccgcc tgcaggagga gctgcccggc gacgagcttc cagcggcgcg cgccgcccga
    15301 aacggccgcg gcgaacccgc cgcacattcc gacgcagtga gcggacgcgg cgaaggtgag
    15361 ggcggcggcg acggcgagct cgttcaagcg gaggcccccg gcagcgggaa ccgcgtcagt
    15421 cgctgcgagt ggacgacgac gaggaggctc gaggcgatca tcgcgactgc gccgaagagc
    15481 gggtgcagga ggccgcagac ggcgagcgtg atgccgatgc cgttgtagaa gaacgcccag
    15541 aagaggttca ggtgcgcggt gcgcacggtc cggtgcgcga gttcgatcgt ccatggcagc
    15601 tttcggaggt cgccgccgag gatcgtcacg tccgccacct cgcgcgcgag gtccgtcccg
    15661 gactccagcg tgacgccggc cgaggcgacg ctcagcgccg gcgcgtcgtt gatcccgtcg
    15721 ccgaccatca cgggcgactc ccccgcccgc accgaagagc ggatcacgtc cagctttccc
    15781 tccggcgaga ggccgccggc cgcgcggaga cccggaaagt ccgccgcgaa cgcggtgacg
    15841 cgcgagggct ggtccccgga gagtatttcg agtgaaagac cgcgcgcccg gagcgccgag
    15901 accgcttcgg cggcctctcg gcgcgtccga tcggagaaga agagaattcc tgcgaatgtg
    15961 cctgagcacg agacataggc cgcagtcctg ccgggaggcg cctcgacgtc ggccacgccg
    16021 gagggtgcgc cgacgaacgc cgcggttccg atccggacgc gtctcccgcc gaccagccct
    16081 tccacgcccc gccccgccac cgcccgaaac tcttccacct tcagcgaaat cttctcttct
    16141 ctttcttttt cccgggcgga cgcggcagag cagatggcgc gccccacggg atgttcgctg
    16201 ccgtgctcga gcgccgccgc gagctccaga agcccctggc ggtcgaagcc cgccgcggga
    16261 cgcacctcgg cgagcgaaag ctcgcccgtc gtgagcgtgc ccgtcttgtc gaggaagacg
    16321 cacttcgccc gggccagcgc ttcgaacacg gcgcccgacc gcacgaggac gcccgccgac
    16381 gcggcgcgtc cgacggccgc cgtcgaggcg agtggcgtcg cgatgccgat cgcgcacggg
    16441 caggcgatga ggaggaccga gagtgcgttc atgagcccct cgcccaagcc tgcccggcgc
    16501 gtccagtacg cgagggtgag cgtcgcgacg aggaggacga acggcacgaa cgcggccgag
    16561 aggcggtcga cgagcggcgc gagcgtgtag cggcccgcgc gcgactcggc aacgagcttc
    16621 acgaggcgcg cgaccgtcgt ctcggcgccc gtgcgccgcg cctcgacgag gagacttcca
    16681 tcctgattga gggtgcccgc agccacggcg tcgcccggtc cgcgcgaggc ggggacgctc
    16741 tcccccgtga tctcggcttc ggagacgctc gccgtgccct cgacgatcgt gccgtccacg
    16801 gcgatgcgct cgccgggccg cacccggatc acgtcgccgg ggcgcagcgc gtccacgctc
    16861 accatctcct cgatcccgtt cacgacgcgc gcggcctccg acgggagctg gcggacgagc
    16921 gccgagaggg cgtcggtcgt gcgggcgcgc gaggaggcct cgaggtaccg cccgatcgtg
    16981 acgaagagaa ggatccccat ggccgtgtcg aggtagatcg ggcccccgcc cgagagaacc
    17041 gaacggactg agagcgcgaa cgcggccgcg acgccgatca cgatgagggc atccgtgtcg
    17101 agggcccggt ccttcaggcg cttgagcccg cggttgagga acggcaggcc gaggaggaac
    17161 agggccgggg tcgcgagcac gagcagcgcc caccggaccc acgcctcgcc cgcggcgcgg
    17221 tccgtctcgg cgccgcgcgt gtacagcacg aagctgcaca tcatcacgtt catcgcgagg
    17281 aacgcgccga cgccgaccgc catcaggagg ccggacgcct gcccgtgccc cgaggcgccg
    17341 ccggtgaggc gatgcgcgag ggcgcagccc ccgcagcagt agcacgggcc gctcggcaga
    17401 cggaagaggc ccgcgaccgg aaggccgcag tgcgcgcaga ggggcagcgg cggaagcgcc
    17461 tggctggacg ccgcggcgac cccgaccgcg ctcaatgccg cggcgccggg gttgcgacgg
    17521 gctggcccgt ctcgaggttg aggaggacgg ggttcgcctt gcgcgcgccg agccagaccc
    17581 agacgaggcc ggcgatcgcg gcgaacgaga cgatccagag gagcgcgtag aggacgcggc
    17641 cctcgctcgg gtggaacgaa gccttcggcg tcttcatcag cccttcctcc ccgcgacgaa
    17701 gagcgcggct tcgaacgtct ccgagcggtc cttcgccttg acggtcagcg tgaacggaac
    17761 gacgctcgac gcgacctccg cgcgcggcac gcgcacgatg atcgtgaacc gtttctccgt
    17821 gcccgccgga acgctcgggt cctgcatccc gtcgagcgcg acgtccttcg gcaggccctc
    17881 ggacgtgacg gcgagcgtca cgggggcttt ccccttgttc gtggcacgca ggaggaaacg
    17941 ggattcggcg aactcggaag gcccggcggc ctgctccgtg tagaccgggg agaggcggaa
    18001 cgagacgtcc tgccggagca cgatcgcgag gacgagggcg acgccgaacc cgaccgtgga
    18061 caccgcgacg aggacgcgct tggcgtccca ctgggtcagc gcggcgctcc cgaagtcgaa
    18121 ggcgaggagg ccgggccgct tcttcttgcc gaggatcagg ttgcaggcgt cgatgcagtt
    18181 gccgcagcgc gtgcattcga tctggtaggg gccgttgcgg atgtcgatct ccatcgggca
    18241 gaccttcgcg cagaggttgc actggatgca cgggccggtg gcttcggagt aggccacgtg
    18301 gaggctccgc ccgtcctcga gaatgccctg caggaggccg taggggcaca ccgaccggca
    18361 gaagcgttcg cgcaggaacg cgaagtcgag gaagaagagg accgcagtga gaagacccag
    18421 gagaccaacc cacggaccga cgtcgaaacg cacgaggcgc cgaacgacct cgggcagggg
    18481 cgcgaaatac gccatgaaaa ggacagaaaa gaggaccgag acgggcgcca gcagcgcgag
    18541 cgacacggcg cgcaccgcgg cccctcttcc attcgaagaa atcttccggg caatcctctt
    18601 cgcgagcgcg tcgaggtcgt tcgcgaactc ggagaagacc atctgcgggc acgcgtacgc
    18661 gcaatagacg cgcccgagga tgagcgaggc ggcccccacg agccacatcg cgaacatgag
    18721 cgcgagccag atgagggtcc actcgtccag ccagatctcg ttccggaaga agtagaagcg
    18781 gctgatcgtg aagtcgaacc ggaagaggtc gaagagcgga aggagggcga agacgacgaa
    18841 gaaccccagc tggatgagtc tccgttgctt cgcgaaccgc cccgtccacc ccggaagcgc
    18901 catggtcagt acttcaatcg cgcgttctgg accggctcga cctggagctc ccggtggaac
    18961 gcgtagcgct gcatcgtgaa cgccctcgtc ggacagcgcg tcgcgcacat cgagcagcgg
    19021 atgcaggccg tctcgtcctt caacatgacc ccgccgaggg cgtcgagctc cgcgggctcg
    19081 aacgcggcga gcgtttcctt cgtgatgccg agctgctccc ccgccatcgc gaggagccgc
    19141 gggtcggagg cgacctgcga gagccccacg aggtggaggc agtcgtccgg gcacacgtcg
    19201 acgcagccgt tgcacgcgat gcagtgctgg ccctcgaagt acgtctggac gtcgcagcgc
    19261 aggcagcgtt cggcctgccg gcgcgcctcg tgctcggggt agttccgctc cacgagccgg
    19321 tcggccgccg ccggcagctc gttcggacgg ggggcctccg acggcggaag gcgccgctcc
    19381 tcgatttccc aatcagggcc catcgtgtat gcggcaggcg accacgcgta gttcaggacg
    19441 acgctcgtcg tcgacttccg gaggtagtcg tggatgctcc gcgcggccac ctgggccgac
    19501 gcgatcgcgt cgatgaagag gcgcgggccg aggacgacgt cgccgccggc gaagacgtcg
    19561 ggacgcgacg tctggagcgt ctcgctgtcg accttgacga ggccgcggtc cgtcagctcg
    19621 atgccgtcct cgggcgtcag gaagttgaac tccgcgctct ggccgatcgc gaagatgacc
    19681 gtgtcgacct cgatcgtctc cctcatggtc tcgtcgaact tcgggttgaa ccgcttctcg
    19741 gagtcgaaga ccgagaggca cgacacgaac tcgacgccgg agaccttgcc gtttccctgg
    19801 atcgcacgcg gcccgagcga cgtgtggagg tggaggccct cgtgcagccc ctcctcgatc
    19861 tcgacggcgt cggccggcat ctcctcccgc gattcgaggc acgcgacgtg cacctcggtg
    19921 acgccggccc ggcgcgccgc cgtgcgggcg acgtcggcgg tcacgcggaa gtcctcggtc
    19981 tcgttcgcgt cgttccggag cgcggagcgc gccacgtcga acgcgacgtt gccgccgccg
    20041 atgacgagga cgcgggggcc gatgtcgatc ttctctccga ggttcaccag cttgaggaac
    20101 tcgagcccgt tcacgacgcc gggcaggtcg gcgccgggga tcttgaggcg ccggctgccc
    20161 tgcaggccgg cgccgatgaa gaacgccttg tacccctggg ccctcagatc ggacatcgtg
    20221 aggcccggcg cgcccacgcg cgcgttcagc cgcagctcga cgccgagcga caggatcgcg
    20281 tcgatctcgg ccttcacgag ctctcggggc agccggtaga gcggcacgcc gagcgtgagc
    20341 atgccgcccg gcacgggatt ggcctcgaac acagtcacgg ggtacccggc ccgggcgagg
    20401 tcgtgggcga ccgtgaggcc gccgacgccc gagccgatca cggcgatctt ttccgcgtag
    20461 ttccccttcg ggacgagcat ccgttcgtcc gcgtagcgat ggtacttctc gtggctgccc
    20521 gtctcgttcc cgtgcagctc gcacgcccag cgcttgacgg cccggatcgc gacgggagca
    20581 tccacgcttc cgcggcggca cgccgcctcg cagggcgcgc cgcagatgcg gccgcagatc
    20641 gagacgaagg ggttcgtcgc ccgcgcggcc gcgtacgcgt cctcgtcccg gccctcggcc
    20701 accgccgtca cgtatccgca ggcgtccgtg aacacggggc agccgacgcg gcacttgacc
    20761 tgctcgaccc agtaccgctc ggcgtccggg atccggagcc gccggtacgc ggaggagatc
    20821 gccgtttcca tggagcccta ctcgccgtcc tctcgggacc ggaacgcgaa ggcgaggagg
    20881 ctcgcgacga acgcgaggag gatcacgccg atgacggcgt gccagcccgc gccgggcacc
    20941 gacatgatcc tgttggcctc ctggacgagc gtcccgcgcg tggagtgcgt cgtctctccg
    21001 gcccagtacg catagaggta ccagaggccg aagaggcaga agccgacgta ggtgaacttc
    21061 aggaaggtcg ggatgggcgt ccccgcacgc tcggtgatcc agcccccggc cagctcgtgg
    21121 atcttctcgt tctcgggctt gtcagccatt tttcgttcct ccgtcgtcgt cgtcgagcat
    21181 ccggaacttc ggggcctcgt cgtccttcat cgcgccggtc ctgatcgccc agaggagcag
    21241 gcagagcgcc gaggcgacga aggacgccat gagggcgtac acgccgaaga tcgggtccca
    21301 gacggcgaac gtcatgttca ctgctcgtag tcgtgcgccg ggcgccagtc cttctggcgg
    21361 ccgagcgact ggacgtaggc gacgagcgcg tcgagctcct tcgcgtcgtt cgcaatccac
    21421 gggaacggcg gcatgacgga gcccggcacg agggcgcgcg ggtccctgaa gtgcgtcacg
    21481 tgccattggc ggtcgtactt tccgccgacg cgcgagaggt ccggtccgac gcgtcgcgtc
    21541 ccgaacatgt gcggggcgtc gtagacgaac tcgtccggcg tcgagacggg gctcttcacg
    21601 ccccgccagc cgtagcggac cgtgtcggcc tcgatcgtgc gcgtctgctg cgtgtggcag
    21661 taccagcagc cctcgcgcac gtagatggcg cgaccctgaa cgacgcgcct tgcctcggca
    21721 tcgcccgatt tcgcgagcac ggcgtagttc ttgaggccgg ggaccggctt cgtccagctc
    21781 gcgtcgaaga agggcggcgc gaacgtcgtg aggatcgcgc cgaccgcgaa gaacccgacc
    21841 gccgctccga tgaagagccc gctcgatttg tcgaccttgc tcatcgcgtg ctccttacgc
    21901 ggcggcgacg gccgcgcggt cgcgccgtcc cgtccacgtc atgaggaggt tccagaagaa
    21961 gatcaggatg ccggtgaaca tgagcgttcc gccgacgagg cggacgagcc agtacggcct
    22021 catgaacttc accgtgtcga tgaacgggat cgtgacgtcg ttccactgca ggccctggag
    22081 gaacccgccg atccacagcg agacgaagaa cgagagcccg ccgatgagcc acagccagaa
    22141 cgtggcgttc gcgagcttgt ccgagtggag cgacgtcttg agcatccgcg ggatcgcgta
    22201 gtacgccgcg ccgatcgcga agaacgagaa cgcgccgagg acggccatgt gggcgtgccc
    22261 gacgatccag tccgtcttcg agacgatcgc gttgacgctg cgcagggagt gcatcggacc
    22321 ctggaagcag gtcaggaggt agaagatcgt ccccgtcatc aggaacttga tcggaacgtt
    22381 gtccttcagc tggtgccact ggcccttcat ggtcgcgagg aagttccaga cgaggctcca
    22441 gaccgggatc atgagcatga cggagaagag gatcgcgacc gtctgcagcc actggctgat
    22501 gggcccgtgc agcatgtggt gcgcgccggt ccacacgtag acgaaggcga gcgaccagaa
    22561 gccgaccatc gaaagccggt gggagtagag cggcgactcg gaggccttcg ggatgaagta
    22621 gtagccgatc ccgaggccca gcggcgtgaa gatgaggccc accgcgttgt gcacgtagaa
    22681 ccagttcagg ttcgcctggt tcgtgcccga cgtgaacatc gtcgcgaagt tcccgacgat
    22741 gtagacgacg gccgtccaga ggatcccgcc gacgatgtac cagatcgcga cgtacatctg
    22801 ctggtacttg cgcgtcacga cggtcatcac gatgttcagg ccgaacatca cccacgcgat
    22861 cgtcacgagg acgtcgagga cgagcgggag ctcggcgtac tcgaggccct gcgtgacgcc
    22921 gcccgcgagc gtcacgacgg cgccgaggac gatcgcgacc cagaggtaca gcgtcgccat
    22981 cccgagcttc tcgctccaga gcttgacacc gcagaggcgc gggatcaggt agaaggacag
    23041 ccccatgtcg gcggcaagga gccagccgaa gagcatcccg ttgacgtgca ggggcctcat
    23101 gcgcccgtac tgcagccacg agatgtttcc gaggagcgtc ggcatgttga gcttcccggc
    23161 gacgatcagg gccacgaggc ccgagatcac gaagaagagc gaggccacga ggatgaagcc
    23221 gaaagcggcc ctgtcctcgt gctcgagacc ctgggccgcg ctgcctggtc tggccgcggc
    23281 cgcgacgctg ctcatgcgct tacctccctg ccgtgttctc gaattcagcg accttcgccg
    23341 gctcgccctt cggggcggga cgggtcaggc tgaggatgta gttgacgacg tccccgatgg
    23401 ccttgtcgtc gagggagaag tcgaagccgg cggccggcat cgccgtcccc tggacaccgt
    23461 acttgatgct ttcgtggagc cgcgtgtagc tcacggacgc gacgaacggc gcgttccgga
    23521 ggttccgcgg gcgcgggacg atgtcctcgg cgttcgggcc gtggccgtcg gccttcttgc
    23581 cgtggcagcc ccagcatcgg ttcaggaaga tcgcctcgcc ccgctcgacg ctctccttcg
    23641 agtacgccac ggggttcgcg gccgggatca cgcggccctt cgggacctgg gccctcgagt
    23701 ccttcgacca cgtcgagagg acgtagtcga cgagcgcgcc cgcctgcgcc tccccgccga
    23761 ggatccggcc ccagggagcc atgctcgtgc ccgggacgcc gttcacgagg ctcgcgacga
    23821 ggcgctcgcg cgtcttcgtc cgcatgaacg ccgccttcgt gaggtcgcgc ggcttcgggt
    23881 cgaggtactc ggcgatcagg ccgttgccct gacccttctc gccgtggcag cgggagcaga
    23941 gcgcggcgta gcgcttctcg ggctcggcgg ggatctccgg cgggcggcgc gacaggagat
    24001 acgccgagag cgcgtcgaag atgtcgtcgg ggagcgggta cggcggcatg agagaaccgg
    24061 gcacgacgct cttggggtcg cggaagtgcg cgagcatcca cttgtcgtcg cgctgcgcgg
    24121 acgtgaacgc gagctcgggc ccgacgcgcc cgtcgcgctc cccgagcttg tggcaggaca
    24181 ggcagccgac gcgctggaga agcgcctcgc cgcgctgggc gggcgcctgg gccgcgaagg
    24241 cgacctccgc gtcgtggccg acgatgagcc ccagcggcag ccacttcggg cgctcggcct
    24301 gggcggcctg gaacttcgcg agcggcgcac gcgagatgtg ggccccctgc tgggccttca
    24361 ggaacgcgac gagcgacatg cgctgctcgt tcgtcaggtc ctgccggggc atcgtcgagg
    24421 tcgggttgtt ccagcggggg ttctcgatct tcgcgtggac gaactcccag cggcggcggc
    24481 gcccgacctc ggagagctcg gggccgattg agccgtagga gaggcccgcg atcgtgtgac
    24541 acgtgtagca ggcgcgctcg aagaagaggc ggcggcctgc gttcacgagc ggcgcacccg
    24601 gccagtcgct ctgcgtgtgg cactggacgc agttggactg gatgagcgtc ttcgggagga
    24661 cgggccacgg ccactcttcg gtgccgtgcg cctgctcggc gtcgacggcg cggccctgcc
    24721 cgtcgtggca gatcgtgcag ccgaacgtct cgaagcggtg gccggggacc ggcgggtgcg
    24781 tgcgcagcgg ctggtcggcc ttcgtgaagc gcgggtcgtc cgcggcgatg tggcaggtct
    24841 ggcagcggtc gatgcgctcg gagccgaagc tgcggacgat ggtctgctcg atcgcgggcc
    24901 ggcgtgcgac aagcgtcgcc ctgaccgcgt cgttcgtgga cgcggcggcg gcgtccttga
    24961 agtacttctt ctggtagtcc tgccagcgcg cgaagaactg gttgttgaag acgtacgcgt
    25021 ggagcgcgag gacgacgacg gagatgaacg cgagggcggc gcgcatcggt tacctccccg
    25081 ccggcgccgt ggagggcgcc ggcgcgggcg gcggcggcgc gtgctcggac acggcgggct
    25141 tgtcgaacgg gctgacgaac gcccagttcg gtccgcggaa gaacgtcccg acgacgacga
    25201 gaacgagatt cacgaggagg agcgtcgtga acgtcgtcgt ggcgaggagg cggtggcgcg
    25261 agaaccagac gccgacgccc ttcgtcccgc ggtcgaggta cgggaggagc aggaggagca
    25321 cgacgaagat cgtcgggatc ccgacgccgc cccagaacgc ggagtacgag accatctcct
    25381 ggaggccgag gaagtaccac ggggccttcg acgggttcgg cggatgcgtc gggtcggcag
    25441 gcgcttcgag cggcgcgttc aggacgaccg cgagcacgag gatgacgagg agcgtcaccg
    25501 cgaagacgaa gagctccgcg atgaagaggt agggccagga cgagacggtg ttgtcgggaa
    25561 cgttcccgac cttcgggaac ggcccccgta cgattcccat gacgccgaac gtcttgttct
    25621 tcgcgagctt ggccttggag gcgaggccct cgagcttggc ctgcagggcg agcgcgttct
    25681 tctcgcccgc ctccggcaga tccgagccct cggggcgcga gagtccgccg tccttgcgga
    25741 tcctccagaa atggacgccg atgaggaccg tcatgaggag cggcaggacc gcgacgtgaa
    25801 ggacgtagaa gcggatgagc gcctcctgcc cgacgacccg gtcgccgagg aggaggaagc
    25861 ggacctgctc gccgacgacc ggggcgtacc ccgcgatcgc ggtgccgacc gtgatcgccc
    25921 agaaggcgag ctggtcccac gggagcagat agcccgtgaa ggaggcgaag agcgtcagga
    25981 ggagcaggac gacgccgacg acccagttga actcgcgggg cttcttgtag ctgcccgtga
    26041 agaacacgcg gcagaggtgc aggaggacga tcgcgaccat cccgtgcgcc gcccaccggt
    26101 gcatgttccg gaggaggacg ccgaaggcga cggtgccgga gaggtcgagc atgcggttgt
    26161 gggcctgctc gaccgacggc acgtaataga acatgaggag gatcccggtc acgagcagga
    26221 tcacgaacag gctgaacgac atgacgccga gaccgagggt gtacgtcgcc cggagagagt
    26281 tcgtgtgcgt cttgaccggg tggatgtgca ggaagaagtt ggtgaacgtc gtctgggacc
    26341 ggcccaggtc ggacgatggg agcgggttgc ggaagatcga gttccagacg tttgcgggga
    26401 gcgaacggag ggtcccgagg aagccctgtt cggatttcat cgcgtgcgcc tcagaccttc
    26461 aggaacgttc cggcgggcac ggcctcggcc gtgtcgacct cgagctcgcc gttcggcgag
    26521 agcgcgatgc ggaaccacgg gaggggccgc ggcgccgggc cgccgaggac ggtgccctcg
    26581 gcgtcgaagc ggctgccgtg gcaggggcag tcgaagccga tctcggtcgt cgcgatgaca
    26641 cagccgaggt gcgtgcacgt gttcgagatc gcgcacatgc cggcctcggt ccggacgatc
    26701 gtgacgcgct gtcgcgtcaa cggaaggcgc gtgccgggcg gataggcctc gggcttcccg
    26761 atccggaagc gcgagggctc cccgtacgtg acgcgcggct tgaagaagag caggttcgag
    26821 aacacggcga ccgcgttcgc gccgaagagg gagacgacgc tcgtgatcgt cagcgccctc
    26881 cggcggctca tcgtcatgct cggcttggcc tcggtcggct tcgtcatgtc cgcctccgcg
    26941 gttcggtgtg cggacccgcc ccgtcgccgc cgtcttcggc gaggtcgggc cagaagagct
    27001 gttccttggt tttctcgagg tcgcggaact gtccgctccg gacgccccac gcccaggcga
    27061 gagcaccggc gatccccatg aagatcccgg ccgcaaataa cattaccgtt atggtcatca
    27121 ttgcggcttc gcactcccga gagggggaac aaccgcttgt gatcccatgg cacaagcatc
    27181 gggaggcggc cacgccgcct ccatagttca cctgcctcat ggagaggcag tcgggcggaa
    27241 gacgcccccc tactggaacc cggtcagggc tcggaccgcg aaaggagggc gaggatctgc
    27301 ttgagttcgg ccaccgcgcg gggcacccgg tcgtccaccg gggcgcctgg ggcggcggcg
    27361 gggacatcga cgggttgcgt cacttcaggg ggaggcagtt cggacgaggc gtccgtgatc
    27421 gccggcacgg ctgctttcgg aggacggggc gggcgcggct tctcgacctt cgggggcttc
    27481 ggcgctgaag gaatcgggac gatcgcggga ttcgtgtccg cgccggcatc cttcgattcg
    27541 gcccttccgc cgctttcgct ttcgaggcgc ttcttggcgc ccgcgatggt gtagccctcg
    27601 tcgtagagga ggcgcttgat ctgctcgatg agcttcagct cgcgcgcgct gtacgtgcgg
    27661 gggccgggga tcgacttgtc gggcgcgagg accgggaact cggactccca gtagcgcagc
    27721 acgtaaggct gcacgtccgc gatgcggcag acatctccga tcttgtaacg ggtttcgccg
    27781 ccggaaccgt tcacttcggc catctgcggg ccgtttttag cacggatttt cggaatttct
    27841 tagaaggtta agaggaaacc ggggattagg aagttcttct ctcctcgttc ttccgtccgt
    27901 ccctgttctt ctcgtccccc ggtttcacga tgaacttcac gggcacgccg tccagcttcc
    27961 agtgctcgcg gatgcggttc tggaggaacc gctcgaacga gaaatggagc gcctcggcac
    28021 ggtccgcgaa cagccgcacg agcggcggct cggaggcgac ctggacggcg taccggatcc
    28081 ggagctcgcg gcccgagcgt cccttcggga gctcgcgctc gaaggctttc ttcaggaccc
    28141 ggttcagctc gccggtggac atgcgcgtcg cgaagcgctt cgcgagggcg tccgcctcgg
    28201 ggaggatccg cccgatgccc ttccccgtct tggcggagat cgggaggaac ggcgcgttgc
    28261 gcaggaagac gaagcgccgg acgacgtcct gcttcatctc gcgaaccttc tcgtggtcct
    28321 tctcgacgag gtcccacttg ttcgcgacga ggaggaggcc gcggcccgcc tcctccgcgt
    28381 agcccgcgat gtgggcgtcc tgcgccgtga tgccgtcccc ggcgtcgaag acgatgacga
    28441 ccaggcgggc gcgctccatg gacttgcgcg ccgccacgac cgagagcacc tcgggggcgt
    28501 ccgtcgtgcg gcccttcttg cggatgcccg ccgtgtcgac gaggacgtac tcgcggccgc
    28561 ccacggacag ccggcagtcg atcgcatcgc gcgtcgtgcc cgcgatctcc gacaccatga
    28621 cccggtcgcg gccgagcagc gcgttcacga tggacgactt cccgacgttc gggcggccca
    28681 cgatcgcgat cgggaggggc tcggtgcggg tgtcggggtc gaggtcgggc gggacggcgg
    28741 cctcgatggc gtcgaagatc tcgtcggcgc ccgtgccgtg cagggccgag acgcccacga
    28801 cctgcgggaa gccgagctgg tagccgtccg ccgtcgagtc cgccgcggac ttcgcgtcca
    28861 ccttgttcca cgcaacgagg accggcttgc cgagggcgtg caggcgctgg gcgatgcgct
    28921 cgtcctccgg caggcgcccc gcgacgccgt cgaggacgaa gatcagaagg tcggcgtcgg
    28981 cgacggtcga cagcgtccgg tcgctcgtcc acgccgcgaa gccgcccgcg gcgtcgaggt
    29041 ccaggccgcc cgtgtcgacg agctcgtagg cccggccgct cggcgtcttc gcgacgaccg
    29101 cgagcgcatc gcgcgtcatt cccggctggt cgtggacgag cgcccgcctc tcgccggcca
    29161 gccggttgaa gagggcggac ttcccgacgt tcgggcgccc gacgaggacg accttgaagg
    29221 cggccatcag cgccgcctgc gcggggcggg cccgcggcgc gcgggccggc gctgctggcg
    29281 atggagcgct tcgacgcgcg tccggctgcc gggccccgcg ctcgagccca ccgggccgaa
    29341 atcgagcccc cgcttctcgg
//
LOCUS       DAOMFY010000017        28948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_518_length_28948_cov_7.182501, whole genome shotgun sequence.
ACCESSION   DAOMFY010000017 DAOMFY010000000
VERSION     DAOMFY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 28948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28948
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_518_length_28948_cov_7.182501"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..567)
                     /locus_tag="PLB01_02890"
     CDS             complement(37..567)
                     /locus_tag="PLB01_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ76276.1"
                     /translation="MSVEVRNLARRFTDDGPPAVADVSFTAPTGAITAVLGPSGAGKS
                     TVLRIIAGLEEPDAGRVLLSGEDVTATPVQKRGVGFVFQGYALFGHLTVRENVAFGLR
                     VRRVPRREAFARADALLARVACHGAVRFGDPLQRAEMTGLLADLDTIPFAATCPHGRP
                     LLIELTRAELQRRVLR"
     gene            complement(564..1406)
                     /gene="cysW"
                     /locus_tag="PLB01_02895"
     CDS             complement(564..1406)
                     /gene="cysW"
                     /locus_tag="PLB01_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HQQ76277.1"
                     /translation="MAASRGFRPAPTLAERPAVRRALVAAALAFLGLFVFLPLASVFA
                     QALRKGPAAYLAAIAEPNAVAAIRLTLLTAAMVVPFHLVFGLLTAWCVAKFRFRGKGL
                     LVTLVDLPFAVSPVIGGLVFVLLFGRRGLLGPFLALHDWKVIYAVPGIVLATIFVTFP
                     FVTREVLAVMEAQGNAEEEAALTLGANGWQTFFRVTLPKVRWGLLYGVVLCNARAMGE
                     FGAVSVVSGHIRGVTNTIPLHVEILYNEYDMTGAFAVASLLTFLAIVTLAVKKVEEAR
                     AASR"
     gene            complement(1437..2240)
                     /gene="cysT"
                     /locus_tag="PLB01_02900"
                     /pseudo
     CDS             complement(1437..2240)
                     /gene="cysT"
                     /locus_tag="PLB01_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
     gene            complement(2237..3361)
                     /locus_tag="PLB01_02905"
     CDS             complement(2237..3361)
                     /locus_tag="PLB01_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQQ76278.1"
                     /translation="MEPNAQSLPRRGNRPAAQRAHHPGRLLVRAAAAAALLFTFKEIS
                     LFSSPSPRSSLLAAPPATTLLNVSYDPTRELYEEINAAFRKRWEPAHGPLTINQSHGG
                     SGKQARAVIDGLEADVVTLALAGDIDAIAEKSGLLPAAWQSRLPQNSAPYTSTIVFVV
                     RKGNPKGIRDWGDLARPGVAVITPNPKTSGGARWNHLAAWGWALRQKGASEATAREFL
                     ARFYKNVPVLDAGARGSTVTFAERGIGDVLVAWENEAFLLTKDVGKTRFEIVVPSVSI
                     LAEPPVALVDRNAEKRGTRAAAQAYLEFLFTDAGQEIVAKHHYRPRSASVAARYAATF
                     PKVALFTIDEAFGGWKKAQRAHFDDGGIFDAVTAATQGAR"
     gene            3454..3855
                     /locus_tag="PLB01_02910"
     CDS             3454..3855
                     /locus_tag="PLB01_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQQ76279.1"
                     /translation="MKLTRRSEYALLALIHLARAEGEGYVAVSAIAEAQGLPAKFLEQ
                     LLMALKRAGLVKSQKGPHGGYRLGRPASKITLAEVVRLLDGALAPTESVSTYFYESTP
                     VEREKRLLKVFKEIRDLVSAKLERTTLADVA"
     gene            3899..4291
                     /locus_tag="PLB01_02915"
     CDS             3899..4291
                     /locus_tag="PLB01_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQQ76280.1"
                     /translation="MASLPFRLDPSSGVPFYRQVIDQVLLAVADGRLKPGVQLPTVRA
                     LAVDLSVNLNTIARAYREMEIRGIVETQQGTGTFVSARKPEKKTAERRKALERLVDQF
                     LTTAATQGFTHEEFAEALLERTEEAGRR"
     gene            4297..5283
                     /locus_tag="PLB01_02920"
     CDS             4297..5283
                     /locus_tag="PLB01_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HQQ76281.1"
                     /translation="MVPTIVPRVTEKIQEARAAAEARRSTPSERRQRQSEKDIQARRY
                     VSGVSLLAFVVALLAGIGVGAAMKSPLPIFGGILLGVFLLFALKVANQWERAVVLRFG
                     KFQGLKGPGLFVLIPVVDEITQLVDQRVRVTDVSAESALTKDTVPVNVDAIVFWTVWD
                     AQKSVLEVADYLDAVALSAQTALRETIGRHQLAEMITEREQLGKQLQTILDEKTNPWG
                     ITVQSVEIRDVRIPADLEDAMSRQAQAERERQARIILGTAETEISARFVEAGDAYKDH
                     PLALHLRAMNMLYEAIKEKGSMVIVPSSAVETMGLGGLSGMTALAQKNQTGE"
     gene            complement(5294..5764)
                     /gene="bfr"
                     /locus_tag="PLB01_02925"
     CDS             complement(5294..5764)
                     /gene="bfr"
                     /locus_tag="PLB01_02925"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HQQ76282.1"
                     /translation="MKGDPKVIEILNDVLCAELTAVNQYFIHGMMCGNWRYQTLAKHG
                     RDESIDEMKHAQKLIERILYLDGVPNMQKYMKINVGQTVPEQHAFDLEVERTAVARLN
                     AGLEICRASGDNGSRMLLEEILKEEEEHIDWLEAQLQQISDLGLPNYLANQAES"
     gene            complement(5768..6004)
                     /locus_tag="PLB01_02930"
     CDS             complement(5768..6004)
                     /locus_tag="PLB01_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQQ76283.1"
                     /translation="MSARPRLLPDAAVPPLMTACACTGRTFEEVERRMHRERLSAVEA
                     ERLTGCGSLCTACLPDLRAYLARISGLEPQTRME"
     gene            complement(6001..6438)
                     /locus_tag="PLB01_02935"
     CDS             complement(6001..6438)
                     /locus_tag="PLB01_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76284.1"
                     /translation="MKRWAAVLNHLACLFVGLSGVAYGIMKYFMAGADPDSRVGHPWQ
                     QPMLKVHVLTAPFLIFALGLVFSGHALKRLREGEDPGRASGAGILGLAAPLVLTGPLI
                     QVLTGDTARQWTGWIHAALGVVYVLAYAAHLLKKRPVGASGNA"
     gene            complement(6435..7085)
                     /locus_tag="PLB01_02940"
     CDS             complement(6435..7085)
                     /locus_tag="PLB01_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HQQ76285.1"
                     /translation="MRRRKEKKEEFPRKEEERGHSLARRAPTARSRPFPLTLKEISLS
                     LLFVVFLSFPVFAKSFLTQEEALRLAFPKGAVVTRKTAFLSDADRAEVARRSGGAPPP
                     GLVAFYAATLDGKDAGTAYFDTHVVRTLPETILVAVDPKGAITRVEVLSFSEPEEYLP
                     RGTWYGQFPGKTLGDELSEKRGLRTVTGATITVRVTVEAARRVLALDAFLKEKSRR"
     gene            complement(7082..8461)
                     /locus_tag="PLB01_02945"
     CDS             complement(7082..8461)
                     /locus_tag="PLB01_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76286.1"
                     /translation="MIRQHSPRGAAAAVRLSLLLSILLFSSPLLADEPPRSSDPAAEQ
                     RMKDLEQKLDVLTKEIEAMRLGEAPSAAPAPAPGAAPAPALDSASRYGLGPAASRVYA
                     RKGVSIGGYGEVLYQNFASSKQDGTPSGVTDTIDLARAVLYFGYKFDDHFVFNSEVEF
                     AHAVAGSDKEGEVEVEFAYVDWLSHSSAIRARAGLLLIPMGFINELHEPPSFLGARRP
                     DVERFILPATWRELGLGGYGEAGPLAYRLYVVNGLNSGGFTADEGIREGRQEGSLAKA
                     QDFALTGRLDFVGVNGLVAGASFFTGCSGQGRTTANGETVCGRTTVWDVHADFRWRGL
                     SLRALFAGSTIGDAATLNALNGLSGDEGVGSRQRGWYAEAGFDVLTLGPRTKLSLTPF
                     VRYEEWDTQASVPDGFSRNPENDASQWTAGVVFKPIPQVVVKLDGQWRKNAASTGVNQ
                     INVALGYEF"
     gene            complement(8458..9432)
                     /locus_tag="PLB01_02950"
     CDS             complement(8458..9432)
                     /locus_tag="PLB01_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQQ76287.1"
                     /translation="MQTLARHLGVMGTALALTVSAPTRGRALDASEAAVRAVEVAERL
                     LSTWRADTPLARLNAAAPGELHETPAALFGLLKKVFEWEEKTGGAFDPTVAPLVSAWG
                     LRSGGRIPGARELLDARRATSTSLFSFEESSSSVSRRHAAAGIDEGAWGKGWALDRAA
                     EAARAAGATAVVLDLGGQVLSSGDETSVAVAHPRDRARTVATIRLKDASASTSGNSER
                     GLAVDGRRVGHILDPRTGEPAPDFGSVTVVAPDGLTADVLSTAFFVLGPEQGLALSAR
                     LRADGVAHETLFFLEGEEGLPLRVSMSPGMRRLVSLSHSKKSSEGVVP"
     gene            complement(9523..9750)
                     /locus_tag="PLB01_02955"
     CDS             complement(9523..9750)
                     /locus_tag="PLB01_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76288.1"
                     /translation="MLYVKDSAQPAAHAGATAALIAASLVSLAGGAILAVPLHGIARF
                     AVGVGGFAGYVALFARALRRLGLPGAPILLP"
     gene            9865..11841
                     /locus_tag="PLB01_02960"
     CDS             9865..11841
                     /locus_tag="PLB01_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQQ76289.1"
                     /translation="MADPSRISHVLLGKPRDVKDPNVFHRVSLVAFLAWVGLGADGLS
                     SSAYGPDEAFRALGEHRSLAIVLVAMTAVTIGVISFAYSNLIQHFPGGGGGYLVATKL
                     LGEKAGVVSGCALLVDYVLTIAVSIASGCDQIWNFLPPHLAPYKLPAEVVVLVFLVVL
                     NLRGVKESVTILAPIFLVFIGTHAFTILYTFAIHLGALPGVFTGAARDWHATKSTMGF
                     WPLAFVLLRAYSMGGGTYTGIEAVSNGVTMLREPRVKTGKKTMLLMALSLAFTAGGIL
                     FGYLLVGARPQEGKTMNFVLFDTLFGAFRPGGIAAGTAFVVVLLVSEAALLFVAAQTG
                     FLDGPRVLGNMAVDSWMPHRFAQLSDRLVTQNGVVVMGVAAGAALLYTRGNISTLVVM
                     YSINVFLTFTLTELGMSRHWIVDRAKEPQWKGLLAIHGTGLLLCVTILAITLYEKFAE
                     GGWMTTVVTSATVTLCFLVRRHYRSVAADLRRLDDVLVPLPGPAAPSTPAPPLDRSLP
                     TAILTVDRFSGYGLHQVLSIHSLFPKYYKNFLFVSAAVVDSGNFKGSDELERLEADAR
                     ANLEKYVAWCRAHGWNAAARLATGTEAVEKVVEMCREAAREFPRSMVFSGKLVFKKEE
                     WYQRLLHNETAMAIQRRLQADGVPAIVLPVRAAV"
     gene            complement(11864..13033)
                     /locus_tag="PLB01_02965"
     CDS             complement(11864..13033)
                     /locus_tag="PLB01_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQQ76290.1"
                     /translation="MKSDRDARPDPESFLRLANRDPRRGHLKVYIGQAAGVGKTYKML
                     DDAHALRGRGVDVVVALVETHGRAETAARVGDLEVVPRRRVAYKGADLEEMDLDAILA
                     RRPEVAVVDELAHTNAPGSKNEKRWQDVEDLLAAGISVATALNVQHLEGVQDVVKSAT
                     GIEIKERVPDRIIREADAVVNVDLPGPELRDRLKEGKIYPPAQAAQALENFFREDNLR
                     ALRELALRETAENVDHGPTEAALSAATSGAFAAASPARVAVALPLDPLVARALIRRGS
                     RLAGRMNTRWYALYVRRKRDRPERLSGREHRELTENIQLAISLGATIVYREAEDVARA
                     LLDFAREEKVKVLVLGRPTRTGITGRIAPGIVSRVLEGARGLDVFVVDVDREAGA"
     gene            complement(13041..13640)
                     /gene="kdpC"
                     /locus_tag="PLB01_02970"
     CDS             complement(13041..13640)
                     /gene="kdpC"
                     /locus_tag="PLB01_02970"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HQQ76291.1"
                     /translation="MKEHLLGSIRMTIVMTALLGFAYPAAVWGVGRLALREEAAGGFL
                     RRIGADAGAPPVGSALIGQFFHSKNLFHSRPSAAGDAGYDATQSGGTNLGPTSKKLAE
                     AIGAAVVAARLENPAAAASPVPADAVTSSASGLDPHISPEYALWQVPRVARETGVPEA
                     DLGRMVAARTEGRFLGLWGAPRVNVLCLNLDLVARGAAP"
     gene            complement(13652..15688)
                     /gene="kdpB"
                     /locus_tag="PLB01_02975"
     CDS             complement(13652..15688)
                     /gene="kdpB"
                     /locus_tag="PLB01_02975"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HQQ76292.1"
                     /translation="MTNTQTTSLFDRAILRRAARDAFAKLRPARMIKNPVMFIVEVGA
                     VATTLFLAPGLAPRGLFGFQLQIVLWLWTTVLFANFAEAVAEGRGKAQADALRRTKSD
                     TVARRLAGAAEERVLSTSLRKGDRVVVETGETIPGDGTVVEGIASVNESAITGESAPV
                     IREAGGDRSAVTGGTTVLSDRIVVEITSEPGQTFLDRMIRLVEGAERQKTPNEIALDI
                     LLAGLTIVFLLATVTLHPFAAYSGGSVSVTVLIALLVCLIPTTIGGLLSAIGIAGMDR
                     VLTHNILAMSGRAVEAAGDVNVLLLDKTGTITLGNRQAVRFVPAPGASEAELAELAQL
                     SSLADETPEGRSIVVLAKEKHGLRARALRDLGATFVPFTAKTRMSGLDFENGRKVRKG
                     AADMVDSFVRGLGGAFPEQVRQDVSCIAAEGGTPLVVADGMRVLGTIYLKDVVKGGMK
                     ERFDRLRAMGIRTVMITGDNPLTAATIAREAGVDDFLAEATPEHKLALIRAEQAKGNL
                     VAMTGDGTNDAPALAQADVGMAMNTGTQAAKEAGNMVDLDSNPTKLIEVVEIGKQLLM
                     TRGALTTFSIANDVAKYFAIIPALFLATYPQLQALNVMRLATPSSAILSAVIFNALII
                     VVLIPLALKGVAYRPLGAGRILRRNLLIYGLGGVGVPFLGIKAIDLLLVALHLV"
     gene            complement(15696..17405)
                     /gene="kdpA"
                     /locus_tag="PLB01_02980"
     CDS             complement(15696..17405)
                     /gene="kdpA"
                     /locus_tag="PLB01_02980"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HQQ76293.1"
                     /translation="MPDVLLKTGVFFLLVLAAAKPLGVFMASVFEGRKTFLDPVLVPV
                     EKAVYRLAGVDASRDQGWRSYGAAMLVFNVVALVVLYALQRLQGVLPFFNPDRLGAVP
                     PALAWNTAVSFTTNTNWQAYAGETTMSHLTQMAGLAVQNFLSAATGMAIAVAFVRGIA
                     RADSRGIGNFWRDLVRATLWVLLPISLVAGVVLMSQGVIQNFQPTRSLTTLEGAIQKI
                     PGGPVASQEAIKMLGTNGGGFFNANSAHPFENPTPFTNLLQMVLIFAIPAGLTVTLGR
                     LTGKPRHGWAVFWAMGLLFAGGALVAGWAETRGNPAHHALGVSQAPGNMEGKEVRFGP
                     VDSALFATVTTDASCGAVNSMHDSFTPLGGLVPLVNIQLGEVVFGGVGAGLYGMLLFV
                     VLSVFIAGLMVGRTPEYLGKRIEAHEVKLAMLSVLILTFCILVGSAAGAATKAGLAGL
                     NNAGPHGLSEILYAYSSAAGNNGSAFAGLNANSDFYDVSLGLAMFLGRFWMIVPLLGI
                     AGSLAAKKRVAESAGTFPVDGPVFVFLLTSVVVVVGALTFFPALSLGPIVEHFLMGAG
                     KLF"
     gene            complement(17413..17502)
                     /locus_tag="PLB01_02985"
     CDS             complement(17413..17502)
                     /locus_tag="PLB01_02985"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HQQ76294.1"
                     /translation="MTFETVLGLVLAAAALAYLLYALLHPEEL"
     gene            complement(17606..18790)
                     /locus_tag="PLB01_02990"
     CDS             complement(17606..18790)
                     /locus_tag="PLB01_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQQ76295.1"
                     /translation="MAKGRNVRREDGLRKAREEALARSAASAAVLAALFFEGNMALGQ
                     TPPPQESGVAAGSTVTLHGFVDAYATWNPNRPSDRNNFEPGTGTTAEKANQFGLNLAA
                     LDVSLDAKPVGFHIVGVAGTGTDVVHAAELHVDTWRHVSQASVSYRLDAGRGVLFELG
                     IQPSHIGYEGFFSKDNWNYTRGWMGEFSPYYQTGLKVATSFDDHWSGQLWLLNGWQNI
                     EDNNDGKAIGTQIAYAKDALGLTVNTFFGPELANDDAHWRSFVDFVGTLKISSKVNIA
                     ASVDLGRQTRPQAAAASWQGASLWLQYLLRKSSSSSSSPSLALALRVEVFHDPDAAVS
                     PVSQTLFGSTLTFELRPHPQVILKLEGRYDRSTADAFGGPDGPLTKREEFLLALGAVA
                     TF"
     gene            complement(18866..19561)
                     /locus_tag="PLB01_02995"
     CDS             complement(18866..19561)
                     /locus_tag="PLB01_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ76296.1"
                     /translation="MTRTLATILVVDDDPAIRRGLAAELQAAGYATLEAADGEEGARR
                     FAEGEPDLVLTDLAMPAVDGFELIARLRKTGNTPILVLSVRGGDADKIRALDLGADDF
                     VVKPFSVPELLARVRAQLRRHSDESAEKVLEFPGLTLDRERRRVVVEGKEVRLTPTEL
                     SILEVLAAHAGKPVTIRQIIGRVWGGAPGTTPDAVRVHVGSLRKKIEPDPARPRYVVT
                     EPWVGYRFVAEPI"
     gene            complement(19558..21030)
                     /locus_tag="PLB01_03000"
     CDS             complement(19558..21030)
                     /locus_tag="PLB01_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HQQ76297.1"
                     /translation="MRRPFTATTSTGRTLGRVGLALLGVGAATIAGLELGANATTVGF
                     VYLVVVLFAAARLGFAAAVAASLAATACFNRFFLPPIGTWTIADPANWVALTVFLLAS
                     VLVSRLVANEQRRAEEAEARRTEMEALYELSVDLFAATNRVGALGDAAGRALRSIGAR
                     GGALVLAPAASPGGEVVVATGDHRFDRSDPLLESVVRSGETAEVPSADGARDAYLPLA
                     LGGRPTGVLLVRGTRADRHALASVGRLVALAVEREKFLEERTHLEALRESDALKTSLL
                     RAVSHDLRTPITALRLGLERLRRSPADTADVDAVSAETERLSRRIDNLLAMARLDAGT
                     YVPKPEPAPPADLFRAALEALPLVLAGRPVGVTVSSSAPDALADPTLAVEILTNLLEN
                     AARASRAGVPLELAAEPHPTDRSRVRFEVRDRGPGASAEVKRIAASGVFAGETGGAGL
                     GLVISASLTRALGGSLALLDRPGGGTIARLDLPAAPEATS"
     gene            complement(20993..22402)
                     /gene="lpdA"
                     /locus_tag="PLB01_03005"
     CDS             complement(20993..22402)
                     /gene="lpdA"
                     /locus_tag="PLB01_03005"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQQ76298.1"
                     /translation="MYDLVVIGSGPGGYVAAIRAAQLGLKTAIVEKDAKVAGVGLGGT
                     CLHRGCIPTKAMLKSASLFDETKNHAASFGVRTAGVELDYPGVVKFRDKVVFKGARGV
                     EYLMKKHKIDVHSGFGRLVAANRVAVTGEAGETTLETKNVLIATGSVPRDLPHLKADG
                     VSILNSDHILKSTSIPKSLLVIGSGAVGSEFASCFARYGSKTVLVEVLPRLLPVEDEE
                     VSKEIEKAFRKKGIAVHVKTGVESVTSNGDGSVTVAAKGPDGGPISWSVEKVLLAVGR
                     KPVTEGLGLEALGVAVERGYVRVDTHQRTNVPGVWAIGDVTPTIWLAHVASAEGIVAA
                     ETMAGHPTVPVNRDQVPGCTYCDPEVASIGLTEAKAKERGYAVKTGKFPFGVLAKTAM
                     EPSPEGFVKIVSDAKYDEILGVHIVGPHATELIAEGAAMLRLEATTEEMIRTIHAHPT
                     LSEALHEAAEAVHGHNIHG"
     gene            22526..24910
                     /locus_tag="PLB01_03010"
     CDS             22526..24910
                     /locus_tag="PLB01_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76299.1"
                     /translation="MIGEVFGRYRIDARLGAGGMGEVWRAEDMRLRRPVALKVLHGAP
                     TAEESARLLREARLASQLSHPNIAVIYEIDEIEREGRMVPFIAMEYVEGRTLAERIAE
                     GPLAAAEALDVASQVASALEAAHARGVVHRDVKPGNVIVDAAGRVKVLDFGLAAWRAP
                     RGFSGETWSAGPAGPTQSIPGAVVGTIAYMSPEQARGVDVDARTDVFSLGVLLWETLA
                     GRRPFEGLNAVEILDRVLHADPPPLSRASAGLPPGLESVVGRMLEKDRERRYATMRDA
                     RVDLEAVARGAARTASGAQAPGGFAPPSNAVAVVAFSNITKSPEDEWLRTGIMETVTA
                     DLKAVPGLAVIGCERICEVEKRLTGQASHGESELAARLGREVGARWVVTGGFQRLGDR
                     VRITARASEIESGEVLTTVKVDGAMGELFELQDRIVTQLAEGFHVAARPSSGAGDATH
                     VIDAYEAFSKGLVNLRAESRESVDRAILFFERAVALDPNYARAWIELGAAYDTKATYL
                     AMPELLERAVQAFERGLALEPRLPRAWRELGGTLVALGREERGFEAIRRALELDPEDG
                     SAHAALGRAYFIGKGMFPEAAASFERALQRNPQGGWYALQLAHVATLLRDFPLADRAA
                     RQAIELQERGLSGREGVLIVGAYMRLGVALALQGRYDEALEECRRELAFLRRVDHALK
                     ERVIVELHVHIGSVLVALGRAESGRAALRVALDAYEERLRMGADDPYTRYYVAAAWAL
                     LGEKERALDALERAASQRHAYTAARAVIDPDFASLRAEDRFKALTGGEPGPRAA"
     gene            24935..27427
                     /locus_tag="PLB01_03015"
     CDS             24935..27427
                     /locus_tag="PLB01_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HQQ76300.1"
                     /translation="MPPDLLRNLLFGFVLVLPASLPAAERPVPGRLPTGETVLPSGRL
                     LTPAGTQTEVAPYPFALALTPDGRRVVAACTGADNQSLHLLDASSGKVLAKEPVKKSW
                     LGLAVSPDGTRVYLAGAGGKNVLVYRLENDRFTAEPPLSLLAPGESAKRDAAPSGLAA
                     SADGKSLWAARILLNDVVRVDLASCTVTAVVPVGVHPYRPVLSPDGSLLAVANWGAAS
                     VSLLDTSSGAAVANVKTLDHPSDLAFSRDGRTLFVAQSNRNLVAAVDLASRSIVRQIS
                     VALGPDGPGTPSADALPDGSTPNALALSPDGRTLFVANADDDAVAVVDVGGDVRAART
                     RGFIPSGWYPAALALSPDGRTLWVANAKGGWSWANAAGGPDPTRKGDDKMAWKKTRTI
                     PGSVSRVDVPSARTLAALTARTYANRRPAARGASPAKASAVVPAAPGAPSPIKHVVYV
                     IRENRTYDQVLGDLPQGNGDPSLVLFGRDVTPNAHALAEEFVLLDNLYCDAEVSADGH
                     NWSMGAYATDFVEKVWPPVYGNVAFSYLFEGNDANATPTNGYLWDAAARAGLTLRNYG
                     EFVGTSAEWSPTALSLEEGAVGALKGNTCPFFPGFDTDILDNARVDVFLKEFRGFVKA
                     KEMPRLLILRLGNDHTVGTKKGGRTPRAMVAENDVALGRLVEAISHSPFWKETAIFVI
                     EDDAQNGSDHVDAHRTVGFVISPYTRRKGFVDSTMYSTVSMLRTMELILGLPPLSQHD
                     ASATPMTNAFADAPDDVPFVHRDTKIPFYEMNPDGAPMQAESNGWDFTREDAAPDLAL
                     NEAIWKSIRGADAEMPAPVNAAWVRLAKTRGD"
     gene            complement(27431..28048)
                     /locus_tag="PLB01_03020"
     CDS             complement(27431..28048)
                     /locus_tag="PLB01_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76301.1"
                     /translation="MNPIPRLAIAGLAVLFLAAPGAADVDGGPNVTRLGKTVMRWRDD
                     SVQIVVGYRHAQAHLDRKWILLDIGLTVWSGKPVEIYREDVWLVMPDGRRLPMPSQRR
                     MAEGIPDLRRMLNEAKVQRDPLNGYFPGAVREERLGFFAIPGEDIVFDKVAVNSRTLA
                     SGDVYFESPNATWAPGIYTLVVENRDVHVRLPLTLGIDVPLERLR"
     gene            28166..28612
                     /locus_tag="PLB01_03025"
     CDS             28166..28612
                     /locus_tag="PLB01_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HQQ76302.1"
                     /translation="MSTSSLVGAWKLLVIEFRSDGQVVYPVGRDAAGMCLYDASGHMS
                     LQIMRVDRPRFALNDHQNGTPEEIAAAFKGYLAYGGTYVEDADHGVVVHHVMHSLFPN
                     WIGTDLVRFFKRAGNRLIVSAPTQTVAGHLMDAMLVWELVSDVERT"
     gene            28614..>28948
                     /locus_tag="PLB01_03030"
     CDS             28614..>28948
                     /locus_tag="PLB01_03030"
                     /inference="COORDINATES: protein motif:HMM:NF020846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HQQ76303.1"
                     /translation="MQRFTLARHMSRRLTLCRRVGPCPADAPVRISVLLHAKDGRALR
                     EAVHAVSDPKGAAYGRHLTPDQVRDLVAPGYQDLDAVLSFLDDAGLRVEGVSGARTAV
                     QASGPAAAVA"
BASE COUNT         4453 a         9818 c        10449 g         4228 t
ORIGIN      
        1 gccgcggccg cgcgacctgc tcgacgtcca tcggtctcat cgcagcacgc ggcgctgcag
       61 ctcggcgcgc gtcagctcga tcagcagcgg acggccgtgc gggcacgtcg ccgcgaacgg
      121 gatcgtgtcg aggtcggcga gcagaccggt catctccgcg cgctgcaacg gatcgccgaa
      181 gcggaccgcg ccgtggcagg cgacgcgcgc gagaagcgcg tcggccctcg cgaaggcctc
      241 ccgccgcgga acgcggcgga cgcgaaggcc gaaggccacg ttctcgcgca cggtcaggtg
      301 gccgaagagg gcatagccct ggaacacgaa cccgacgccc cgcttctgga cgggcgtcgc
      361 cgtgacgtct tcgcccgaaa ggaggacgcg gcccgcgtcc ggctcctcga ggcccgcgat
      421 gatccggagg accgtcgact tgcccgcacc cgaggggccg aggacggcgg tgatcgcgcc
      481 cgtgggcgcc gtgaacgaca cgtcggcgac ggcgggaggg ccgtcgtccg tgaagcggcg
      541 ggcgaggttc cggacctcga cgctcatcgc gaggccgccc gggcttcttc gaccttcttg
      601 acggcgagcg tcacgatcgc gagaaacgtc agcagggacg cgacggcgaa ggcgcccgtc
      661 atgtcgtact cgttgtagag gatctcgacg tgcagcggga tcgtgttcgt gaccccgcgg
      721 atgtgccccg agacgaccga cacggcgccg aactctccca tcgcgcgcgc gttgcagagg
      781 acgacgccgt agaggagccc ccagcgcacc ttcgggagcg tcacgcggaa gaacgtctgc
      841 cacccgttcg cgccgagcgt gagcgcggcc tcctcctcgg cgttgccctg cgcctccatc
      901 acggcgagga cttcgcgggt cacgaacgga aacgtcacga agatcgtcgc gaggacgatg
      961 ccgggcaccg cgtagatcac cttccagtcg tggagggcga ggaacggccc gaggagcccg
     1021 cgacggccga agaggaggac gaagacgagc ccgccgatga ccggggagac cgcgaacgga
     1081 aggtcgacga gcgtgacgag gaggcccttg ccgcggaacc ggaacttcgc gacgcaccac
     1141 gcggtcagga ggccgaacac gaggtggaac ggcacgacca tcgccgccgt gaggagcgtg
     1201 agccggatcg cggcgacggc gttcggctcg gcgatcgcgg cgaggtacgc ggccgggccc
     1261 ttccgcaggg cctgggcaaa aaccgacgcg agcgggagga agacgaagag gccgaggaac
     1321 gcgagggcgg ccgcgacgag ggcccggcgg acggcgggcc gctcggcgag cgtcggcgcg
     1381 gggcggaacc cgcggctcgc cgccatcgcc gacgctccgg cgagccggcg cgtccagcgc
     1441 tggagggcgt tcgcggcgaa gacgagcgtg aacgacgcga ggaggaggac gaccgcgatc
     1501 gccgtggcgc cggcctcgtc gaactgctcc agcttcgtga cgatcaggag aggcgcgatc
     1561 tcggtcttca gcgggaggtt gcccgagatg aatacgatcg agccgtattc cccgagtgcg
     1621 cgcgcgaacg cgagcgtgaa ccccgtgagg aggggcggga ggaggttcgg gaggacgacg
     1681 cgaaggaagg cctgcagccg cgaggccccg aggacgcccg cggcctcctc gagctcgggg
     1741 tcgaggtcct cgaggacggg ctggacggcg cgcacgacga agggcaggcc gacgaacgtc
     1801 agggcgagga cgacgccggc cggcgcgaac gcgaccttca ggccgatcgg ctcgaggaac
     1861 cggccgatcc acccctggcc cgagtagagc gccgtgagcg tgaggcccgc gacggccgtc
     1921 gggagggcga acggaaggtc cacgaacgcg tcgaggagcg cgcggccgcg gaagcggtag
     1981 cgcgcgagga cccacgcgac gaggaggccg aagacggcgt tcaggagcgc ggcgaggagc
     2041 gccgcgccga agctcagccg gtaggccgcg agggcgcgcg gggccgtgac gattcgcagg
     2101 aagtccgcgc ccgtgaggct cgcggccttg agcgcgagcg cggcgagggg gaggacgacg
     2161 acgaggccca cgtagagcga ggccagggag agcgccggcg cgaagccggg gaggattccg
     2221 tgccggcgcg tcgtcgtcac cgcgcgccct gcgttgcggc cgtcacggcg tcgaagatgc
     2281 cgccgtcgtc gaagtgggct ctctgcgcct tcttccagcc gccgaacgcc tcgtcgatcg
     2341 tgaagagcgc gactttcggg aacgtcgcgg cgtagcgcgc ggcgaccgac gccgagcgcg
     2401 ggcggtagtg gtgcttcgcg acgatctcct gccccgcatc cgtgaagagg aactcgagat
     2461 aggcctgcgc cgcggcgcgc gtgccgcgct tctcggcgtt cctgtccacg agggcgaccg
     2521 gcggctcggc gaggatcgag accgacggca cgacgatctc gaaccgggtc ttcccgacgt
     2581 ccttcgtgag gaggaacgcc tcgttctccc acgcgacgag gacgtcgccg atgccgcgct
     2641 cggcaaaggt gacggtcgag ccccgtgcac cggcgtcgag gacggggacg ttcttgtaga
     2701 agcgcgcgag gaactcgcgc gccgtcgcct cgctggcgcc cttctgtcgg agcgcccagc
     2761 cccacgcggc gagatggttc cagcgcgcgc cgcccgatgt cttcgggttc ggggtgatca
     2821 cggcaacgcc cggccgcgcg aggtcgcccc agtcgcgaat tccctttgga ttgcctttgc
     2881 ggacgacgaa gacgatggtc gacgtgtacg gggcgctgtt ctgcggcagg cgggactgcc
     2941 acgcggccgg aaggaggccg ctcttctcgg cgatcgcgtc gatgtcgccc gcgagcgcga
     3001 gcgtcacgac gtccgcctcg agcccgtcga tgaccgcgcg ggcctgcttg cccgaaccgc
     3061 cgtgcgactg gttgatcgtc agcggcccgt gggcgggctc ccatctcttc cggaacgcgg
     3121 cgttgatctc ctcgtagagc tcccgcgtgg ggtcgtacga gacgttcagg agggtcgtcg
     3181 ccggaggggc cgcgagaagc gaagaccggg gagaagggga agagaagaga gagatttctt
     3241 tgaaggtgaa gagaagagca gccgccgcgg cggcgcgcac gaggaggcgt cccgggtggt
     3301 gcgccctctg ggcggcgggg cggttgccgc gacgcggcag gctctgagcg ttcggttcca
     3361 tgcccctctt accctctaag aaaatcttct ctttctttat attatttatc ccactaacta
     3421 agtcaagtac tattggcgcg agagaatgcc gccgtgaagc tcacccgccg cagcgaatac
     3481 gcgcttctcg cgctcatcca tctcgcgcgg gcggagggcg aggggtacgt cgccgtctcg
     3541 gccatcgccg aggcgcaggg gctgcccgcg aagttcctcg agcagctcct gatggctctc
     3601 aagcgcgcgg gcctcgtgaa gagccagaag gggccgcacg gcggctaccg gctcgggcgc
     3661 ccggcgtcga agatcaccct ggccgaagtc gtccggctcc tggacggggc cctggctccc
     3721 accgagtccg tcagcacgta tttctacgaa tccacccctg tggagcgcga gaagaggctc
     3781 ctcaaggtct tcaaggagat ccgggacctg gtctccgcga agctcgagcg gacgacgctc
     3841 gccgacgtcg cgtgagccgg cccttgacat tgtactagta gactagtaca ttagggacgt
     3901 ggcgagcctc ccgttccgcc tcgacccgtc gtccggcgtg ccgttctacc ggcaggtcat
     3961 cgaccaggtc cttctcgccg tcgcggacgg gcggctgaag ccgggcgtcc agctcccgac
     4021 ggtccgggcg ctcgccgtgg acctctccgt gaacctgaac acgatcgcgc gcgcctaccg
     4081 cgagatggag atccgcggga tcgtcgagac gcagcagggg accggcacgt tcgtctccgc
     4141 tcgcaagccc gagaagaaga ccgccgagcg ccggaaggcc ctggagcgcc tcgtcgacca
     4201 gttcctgacg accgcggcca cccaggggtt cacgcacgaa gaattcgccg aagccctcct
     4261 cgaacgcacc gaagaggccg gcaggaggta acgaccatgg tgcccacgat cgttccgcgg
     4321 gtgaccgaaa agatccagga ggcgcgagcc gccgccgagg cgcgtcgttc gacgccctcg
     4381 gagcgccgcc agcggcagag cgagaaggac atccaggcgc gccgttacgt gagcggcgtg
     4441 agcctgctgg cgttcgtcgt cgcgctgctc gccggcatcg gggtgggggc cgccatgaag
     4501 tcccccctcc cgatcttcgg cgggatcctc ctcggcgtct tcctgctgtt cgcgctcaag
     4561 gttgcgaacc agtgggagcg cgccgtcgtc ctgcgtttcg gaaagttcca gggcctcaag
     4621 gggccgggac tcttcgtcct gatcccggtc gtcgacgaga tcacgcagct cgtggaccag
     4681 cgcgtccgcg tgacggacgt ctcggccgag tcggctctca cgaaggacac ggttcccgtg
     4741 aacgtggacg cgatcgtctt ctggaccgtc tgggacgccc agaagtcggt cctcgaggtg
     4801 gcggactacc tcgacgccgt cgcgctctcg gctcagacgg cgcttcgcga gacgatcggc
     4861 cggcatcagc tcgccgagat gatcacggag cgcgagcagc tcggtaagca gctccagacg
     4921 atcctcgacg agaagaccaa cccctgggga atcacggtcc agtcggtcga gatccgggac
     4981 gtgaggatcc cggcggacct cgaggacgcg atgtcgcgcc aggcgcaggc ggagcgcgag
     5041 cgccaggccc gcatcatcct cggcaccgcg gagacggaga tctcggcgcg cttcgtcgag
     5101 gccggggacg cctacaagga ccacccgctc gcgctccacc tgcgcgcgat gaacatgctc
     5161 tacgaggcga tcaaggagaa gggctccatg gtcatcgtcc cgtcgagcgc cgtcgagacg
     5221 atgggcctgg gcgggctctc gggcatgacg gccctcgcgc agaagaacca gacgggagag
     5281 tgaagacggg gggctagctc tccgcctggt tcgcgaggta gttcgggagg ccgaggtccg
     5341 agatctgctg aagctgggcc tcgagccagt cgatgtgctc ctcctcctcc ttcaggatct
     5401 cctccaggag catccgcgag ccgttgtcgc ccgacgcccg gcagatctcg agccccgcgt
     5461 tcaggcgcgc gacggcggtg cgctcgacct cgaggtcgaa ggcgtgctgt tcgggcaccg
     5521 tctggccgac gttgatcttc atgtacttct gcatgttcgg gacgccgtcg aggtagagga
     5581 tccgctcgat cagcttctgc gcgtgcttca tctcgtcgat ggactcgtcg cggccgtgct
     5641 tggcgagggt ctggtaacgc cagttcccgc acatcattcc gtggatgaag tactggttga
     5701 cggccgtgag ctcggcgcac agaacgtcgt tgaggatttc gatgaccttg gggtcgcctt
     5761 tcatcgttca ctccatcctt gtttggggct cgaggccgga aatccgggcc aagtatgcgc
     5821 gaaggtcggg gagacacgcc gtgcagaggg agccgcaccc cgtgaggcgc tcggcctcga
     5881 ccgcggagag gcgctcgcgg tgcatccggc gctcgacctc ttcgaaggtg cggcccgtgc
     5941 aggcgcaggc cgtcatcagc gggggaaccg ccgcatcggg gaggaggcga ggccgcgcgc
     6001 tcatgcgttc cccgaggcgc ccaccggtcg cttcttgagg agatgagcgg cgtatgcgag
     6061 cacgtagacg acgccgagcg cggcgtggat ccagccggtc cactgccgcg cggtgtcgcc
     6121 cgtcagcacc tggatcagag ggcccgtcag gacgagcggc gccgcgaggc cgaggatgcc
     6181 cgcgccactc gcgcggcccg ggtcctctcc ctcccgcagg cgcttgagcg catgcccgct
     6241 gaagacgagg ccgagcgcga agatcaggaa gggcgccgtc aggacgtgaa ctttcagcat
     6301 cggctgctgc cacgggtgcc cgacgcgcga atccgggtcc gcgccggcca tgaaatactt
     6361 catgatcccg tacgcgacgc cggagaggcc cacgaaaagg cacgcgagat ggttcaggac
     6421 cgcggcccag cgcttcatcg gcgggatttc tccttgagaa acgcgtccag cgcaaggacg
     6481 cgccgcgccg cctcgaccgt gacgcgcacc gtgatcgtcg cgcccgtcac ggtccggagg
     6541 cccctcttct ccgagagctc gtcgccgagc gtcttcccgg ggaactgccc gtaccacgtc
     6601 ccgcgcggga ggtattcctc gggctcggag aaggagagga cctcgacgcg cgtgatcgcg
     6661 cccttcgggt ccacggccac gagaatcgtc tcggggagtg tgcggacgac gtgcgtgtcg
     6721 aagtacgcgg tgcccgcgtc ctttccgtcc agcgtcgcgg cgtaaaacgc gacgagcccg
     6781 ggaggcggcg cgccgcccga gcggcgcgcg acctcggcgc ggtccgcatc ggacaggaac
     6841 gccgtcttcc gcgtcacgac cgcgcccttc gggaacgcga ggcggagagc ttcctcctgg
     6901 gtgaggaagc tcttcgcgaa gaccgggaac gagaggaaga cgacgaagag aagagagaga
     6961 gagatttctt tgagggtaag agggaaaggg cggctgcgcg ccgtcggcgc tcgcctcgcg
     7021 aggctatggc cgcgctcctc ttcctttcta ggaaactctt ctttcttctc ttttcttctt
     7081 ctcaaaactc ataccccagc gcgacgttga tctggttgac gcccgtcgac gcggcgttct
     7141 tccgccattg tccgtcgagt ttcacgacga cctgcggaat cggcttgaag acgacgccgg
     7201 ccgtccactg gctcgcgtcg ttctccgggt tgcgggagaa cccgtccggc acggacgcct
     7261 gcgtgtccca ctcctcgtag cggacgaacg gcgtgagcga gagcttcgtg cgcggcccga
     7321 gcgtgaggac gtcgaagccg gcctcggcgt accagccgcg ctgacggctg ccgacgccct
     7381 cgtctcccga gaggccgttc agcgcattca gggtcgccgc gtcgccgatc gtcgagccgg
     7441 cgaagagggc gcgcaacgag agtccgcgcc accggaagtc ggcatggacg tcccagacgg
     7501 tcgtgcggcc gcagacggtc tcgccgttcg ccgtcgtccg gccctggccc gaacagcccg
     7561 tgaagaacga cgcgcccgca acgaggccgt tgacgcccac gaagtccagc cgaccggtga
     7621 gtgcgaagtc ctgcgccttg gcgagcgagc cttcctggcg gccctcgcgg atgccctcgt
     7681 ccgccgtgaa gcctccggag ttcaggccgt tcacgacgta gagccggtat gcgagcgggc
     7741 ctgcctcgcc gtagcctccg aggccgagct cccgccaggt cgccggcagg atgaaccgct
     7801 cgacgtcggg gcgccgggcg ccgaggaagg agggtggctc gtgcagctcg ttgatgaacc
     7861 ccatcgggat cagcaggagg cccgcccggg cgcggatggc gctcgagtgg ctgagccagt
     7921 ccacgtaggc gaactcgacc tcgacctcgc cctccttgtc ggatcccgcg acggcgtggg
     7981 cgaactccac ctcggagttg aagacgaagt ggtcgtcgaa cttgtagccg aagtagagga
     8041 cggcgcgggc gaggtcgatc gtgtccgtga cgccggacgg cgtcccatcc tgcttagacg
     8101 acgcgaagtt ctggtacagg acctcgccgt agccgccgat cgagacgcct ttccgcgcat
     8161 aaacgcggga ggcggccggg ccgaggccgt agcgcgaggc cgagtcgagg gcaggcgcgg
     8221 gagcggcgcc gggcgcaggc gccggggctg cggagggcgc ctcgccgagc cgcatcgcct
     8281 cgatctcttt cgtgaggacg tcgagcttct gctcgaggtc cttcatccgc tgctccgcgg
     8341 ccgggtcgga cgacctggga ggctcgtcgg cgagaagggg agaagagaag agaagaatgc
     8401 tgagtaagag agaaagacgg acggccgcgg cggcaccgcg cggcgagtgc tgtcgaatca
     8461 agggacaact ccttcagaag atttctttga atgagaaaga gaaacgaggc gcctcatccc
     8521 gggcgacatg gaaacccgaa gcgggagccc ctcttcacct tccaggaaaa aaagcgtttc
     8581 atgagccact ccgtccgcac gaagtcgtgc cgacagcgcc aggccctgtt ccggcccgag
     8641 gacgaagaac gccgtcgaga ggacgtcggc cgtgagaccg tccggcgcga cgacggtcac
     8701 ggagccgaag tccggggccg gctcgccggt gcgcgggtcg aggatgtggc cgacgcggcg
     8761 cccgtcgacg gcgaggccgc gctcggagtt gccggacgtc gaggccgagg cgtccttgag
     8821 ccggatcgtc gcgacggtgc gcgcccggtc gcgcggatgc gcgacggcga cggaggtttc
     8881 gtctccggac gagaggacct ggccgccgag atcgagaacg actgccgtgg cccctgcggc
     8941 tctcgccgct tcggccgccc ggtcgagagc ccagcccttg ccccaggctc cctcgtcgat
     9001 gccggccgcg gcatggcggc gggaaacgga agaagaggac tcctcgaatg aaaagaggga
     9061 cgtactcgtg gcgcggcggg cgtcgagcag ctcccgagcg ccggggatgc ggcctcccga
     9121 cctgagcccc cacgcgctca cgaggggcgc gaccgtcggg tcgaacgcgc cgcccgtctt
     9181 ctcttcccat tcaaagacct ttttcagcag cccgaagagc gcggcaggcg tctcgtgcag
     9241 ctcgcccggc gccgccgcgt tcagccgggc gaggggagtg tcggcgcgcc acgtggagag
     9301 gagcctttcg gccacctcga ccgcgcgcac cgccgcctcg ctcgcgtcga gcgcgcggcc
     9361 gcgcgtcggc gccgagaccg tcagcgcgag cgcggttccc atgacaccga ggtggcgggc
     9421 gagggtctgc atggtctccc gtggggcttt ggattctgag actaagtctc agtcgcccga
     9481 tggtgccgca cgacccgcgg tccgtcaact gatgcagctg ccctatggca ggaggatcgg
     9541 cgcgcccggg agacccagcc tccggagggc gcgcgcgaag agggcgacgt accccgcgaa
     9601 ccctccgacg ccgacggcga agcgcgcgat cccgtggagc gggacggcga ggatcgcgcc
     9661 tcccgcgagg ctcacgagcg aggccgcgat cagggcggcg gtggcgcccg cgtgcgcagc
     9721 cggctgggcg gaatccttca cgtacaacac gttgggactc gcgacccgag gaagtccgga
     9781 gaaaggtcta aagaatccct aaagaaaggc gccgcgcggg cgcggcgcgg cgcaaactgt
     9841 agggggccgc ctctgcggcc cacgatggcc gatcccagcc gaatctcgca cgtcctcctc
     9901 ggcaagcccc gcgacgtcaa ggacccgaac gtcttccatc gggtgtccct ggtggcgttc
     9961 ctcgcgtggg tgggcctcgg cgccgacggg ctctcgtcct cggcctacgg ccccgacgag
    10021 gcgttccgcg ccctcggcga gcaccgctcg ctcgcgatcg tcctcgtcgc gatgaccgcc
    10081 gtgacgatcg gcgtcatttc cttcgcgtac tccaacctca tccagcactt cccgggcggt
    10141 ggcggcggct acctcgttgc gacgaagctt ctcggcgaga aggccggcgt cgtttcgggc
    10201 tgcgcgctcc tcgtcgacta cgtcctcacg atcgccgtct cgatcgcctc ggggtgcgac
    10261 cagatctgga acttcctgcc tccgcacctc gcgccgtaca agctcccggc ggaggtcgtg
    10321 gtcctcgtct tcctcgtcgt cctgaacctg cgcggcgtca aggagtccgt cacgatcctc
    10381 gcgccgatct tcctcgtctt catcggcacg cacgcgttca cgatcctcta cacgttcgcg
    10441 atccacctcg gtgcgctgcc gggcgtcttc accggcgcgg cgcgcgactg gcacgcgacg
    10501 aagtcgacga tgggcttttg gccgctcgcg ttcgtcctcc tgcgcgccta ttcgatgggc
    10561 ggcggcacgt acacgggcat cgaggcggtc tcgaacggag tcacgatgct ccgcgagccg
    10621 cgcgtgaaga cgggcaagaa gacgatgctt ctcatggccc tgtccctcgc gttcacggcc
    10681 ggcgggatcc tcttcggcta cctcctggtc ggggcgcgcc cccaggaggg gaagacgatg
    10741 aacttcgtcc tcttcgacac gctcttcggc gcgttccggc ccggcgggat cgcggccggg
    10801 acggcgttcg tcgtcgtcct cctcgtctcg gaggcggcgc tcctcttcgt cgcggcccag
    10861 acgggctttc tcgacggacc gcgcgtcctc gggaacatgg cggtcgactc gtggatgccc
    10921 caccggttcg cgcagctctc ggaccgcctc gtgacgcaga acggcgtcgt cgtcatggga
    10981 gtcgcggcgg gcgccgcgct cctctacacg cgcgggaaca tctcgaccct cgtcgtcatg
    11041 tactcgatca acgtcttcct gacgttcacg ctcacggagc tcgggatgtc ccgccactgg
    11101 atcgtcgacc gggcgaagga accccagtgg aagggcctgc tcgcgatcca cggaaccggg
    11161 ctgctcctct gcgtgacgat tctcgcgatc accctctacg agaagttcgc ggagggcggg
    11221 tggatgacga cggtcgtgac gagcgcgacg gtcacgctgt gcttcctcgt gcggcggcac
    11281 taccggagcg tggcggccga cctcaggcgg ctcgacgacg tcctcgtccc gctgccgggc
    11341 ccggcggccc cgtcgacgcc cgcgccgccc ctcgaccgct cgctcccgac ggcgatcctc
    11401 accgtggacc gcttctcggg ctacggcctc caccaggtcc tgagcattca ctcgctcttc
    11461 ccgaagtact acaagaactt cctcttcgtc tcggcggcgg tcgtcgactc gggcaacttc
    11521 aagggcagcg acgagctcga gcggctcgaa gccgacgcgc gcgcgaacct cgaaaagtac
    11581 gtcgcgtggt gccgcgcgca cggctggaac gcggccgcgc gcctcgcgac cgggacggaa
    11641 gccgtcgaga aggtcgtcga gatgtgccgc gaggccgccc gggagttccc gcgttcgatg
    11701 gtcttcagcg ggaagctcgt cttcaagaaa gaggagtggt accagcgtct cctccacaac
    11761 gagaccgcga tggcgatcca gcgccgcctg caggccgacg gcgtgcccgc gatcgtgctc
    11821 cccgtgcgcg cggccgtctg accgcgccgc cgcggatgga ggatcaggcc ccggcctcac
    11881 gatcgacgtc gacgacgaag acgtccagcc cgcgcgcccc ctcgaggacc cgggagacga
    11941 tccccggcgc gatccggccc gtgattcccg tgcgtgtcgg ccggccgagc accagcacct
    12001 tcaccttctc ctcgcgcgcg aagtcgagca gggcgcgcgc cacgtcctcg gcctcgcgat
    12061 agacgatcgt cgccccgagg gagatcgcga gctggatgtt ctccgtcagc tcgcggtgct
    12121 cacggcccga gaggcgctcc gggcggtcgc gcttccggcg cacatagagc gcgtaccagc
    12181 gcgtgttcat ccggcccgcg aggcgcgagc cgcgccggat cagcgcgcgc gccacgagcg
    12241 ggtcgagcgg cagcgcgacg gcgacgcgtg cgggggaggc cgcggcgaac gcgcccgagg
    12301 tcgcagccga gagcgccgcc tccgtgggcc cgtggtcgac gttctcggcg gtctcgcgga
    12361 gcgcgagctc ccgaagggcc cggaggttgt cctcgcgaaa gaagttctcg agcgcctgcg
    12421 ccgcctgggc cggaggatag atcttgcctt ccttcagacg gtcgcggagc tcgggccccg
    12481 gaaggtcgac gtttacgacg gcatccgcct cgcgaatgat gcggtcgggg acgcgctcct
    12541 tgatctcgat gccggtcgcg gacttcacga cgtcctgcac gccctcgagg tgctggacgt
    12601 tcagggccgt cgcgacggag atcccggccg cgaggagatc ctccacgtcc tgccagcgct
    12661 tctcgttctt cgagccgggc gcgttcgtgt gcgcgagttc gtccacgacc gcgacctcgg
    12721 gccggcgcgc gaggatcgcg tcgaggtcca tctcctcgag gtccgcgccc ttgtacgcga
    12781 cgcgccgccg cgggacgact tcgaggtcgc cgacgcgcgc ggcggtctcg gcccggccgt
    12841 gagtctcgac gagggcgacg accacgtcca cgccccgccc acgcagcgcg tgggcgtcgt
    12901 cgagcatttt gtaggtcttg ccgacgcctg ccgcctgccc gatgtagacc ttcaggtgcc
    12961 cgcgcctcgg gtcccggttc gcgagcctca ggaaagactc gggatcgggg cgtgcgtcgc
    13021 gatcggactt cacgccggaa ttatggggcg gcgccgcgcg ccacgaggtc gaggttcaga
    13081 cagaggacgt tcacgcgcgg cgcgccccag agacccagga aacggccctc ggtgcgtgcg
    13141 gcgaccatcc gcccgaggtc ggcctccggg acgccggtct ctcgggcgac gcgggggacc
    13201 tgccacaggg cgtactcggg ggaaatgtga ggatcgaggc cggacgcgga cgacgtgacc
    13261 gcgtcggcgg gcacgggcga agccgcggcg gcgggattct cgagacgcgc ggcgacgacg
    13321 gccgctccga tcgcctcggc gagtttcttc gaggtgggcc cgaggttcgt gcccccggat
    13381 tgcgtcgcgt cgtatccggc gtcgccggct gcggagggac gggagtgaaa gagattcttc
    13441 gagtggaaga actggccgat gagcgcggag ccgacgggcg gagcgccggc gtccgctccg
    13501 atccttcgaa ggaatccccc ggcagcctct tcccggagtg cgagccgccc cacgccccag
    13561 acggcggcgg ggtatgcgaa cccgagcagc gccgtcatca cgatcgtcat ccggatcgag
    13621 ccgagaagat gctctttcat gcgcttgtgc ctcaaaccag gtgaagcgcg acgaggagga
    13681 ggtcgatcgc cttgatcccg aggaacggca cgccgacgcc tccgagcccg tagatcagga
    13741 gattgcggcg gaggatgcgg cccgcgccga gcggccggta cgccacgccc ttcagagcga
    13801 ggggaatgag cacgacgatg atgagcgcgt tgaagatgac cgcggagagg atcgcgctcg
    13861 acggcgtcgc gaggcgcatg acgttcagcg cctgcagctg agggtaggtc gcgagaaaga
    13921 gcgccgggat gatggcgaag tacttcgcga cgtcgttggc gatcgaaaac gtcgtcaacg
    13981 ctccgcgcgt catgaggagc tgcttgccga tctcgacgac ctcgatgagc ttcgtggggt
    14041 tcgagtcgag gtcgaccatg ttgccggcct ccttcgcggc ctgcgtcccc gtgttcatcg
    14101 ccatgccgac gtcggcctgc gcgagagcgg gcgcgtcgtt cgtcccgtcg cccgtcatcg
    14161 cgacgaggtt gcccttcgcc tgctcggcgc ggatgagtgc gagcttgtgc tccggcgtcg
    14221 cctcggcgag gaagtcgtcg acgcccgcct cgcgggcgat cgtcgcggcc gtcagcgggt
    14281 tgtcgcccgt gatcatgacg gtccggatgc ccatcgcgcg aagcctgtcg aagcgctcct
    14341 tcataccgcc cttgacgacg tccttgaggt agatcgtccc gaggacgcgc atgccgtccg
    14401 ccacgacgag cggcgttccg ccctcggcgg cgatgcagga cacgtcctgc ctcacctgct
    14461 ccgggaacgc tcccccgagc ccgcggacga acgagtcgac catgtccgcc gcgcccttgc
    14521 gcaccttcct gccattctcg aaatcgaggc cggacattcg cgtcttcgcc gtgaacggga
    14581 cgaacgtggc ccccagatcc cgcagcgccc gcgccctgag accgtgcttc tccttcgcga
    14641 ggacgacgat cgaacggccc tcgggagttt cgtcggcgag ggaggagagc tgcgcgagct
    14701 cggcgagctc cgcctccgac gcgccgggtg ccgggacgaa gcggacggcc tggcggttcc
    14761 cgagcgtgat cgtccccgtc ttgtcgagga gaaggacgtt cacgtcgccc gccgcctcga
    14821 ccgcccggcc cgacatcgcg aggatgttgt gcgtcaggac gcggtccatg cccgcgatgc
    14881 cgatcgcgga caggagcccg ccgatcgtcg tcgggatcag gcacacgagg agggcgatca
    14941 ggaccgtcac ggagacggag ccgcccgagt acgccgcaaa cggatgcagc gtgacggtcg
    15001 cgaggaggaa cacgatcgtc aggcccgcca gaaggatgtc gagggcgatc tcgttcggcg
    15061 tcttctggcg ctcggcgccc tcgacgagcc ggatcatccg gtcgaggaac gtctggccgg
    15121 gctcggaggt gatctcgacg acgatgcggt ccgacaggac ggtcgtcccc ccggtgacgg
    15181 ccgagcggtc gccacccgcc tcgcggatca cgggcgcgga ctcgccggtg atcgcggact
    15241 cgttcacgga tgcgatgcct tccacgacgg tcccgtcgcc ggggatcgtt tcgccggtct
    15301 cgacgacgac gcggtcgccc ttgcgaagcg acgtggagag cacgcgctcc tcggcggcgc
    15361 cggcgagacg gcgggcgacc gtgtccgact tcgtccgccg gagcgcgtcg gcctgcgcct
    15421 tgccgcggcc ctcggcgacg gcctcggcga agttcgcgaa gaggaccgtg gtccacagcc
    15481 agaggacgat ctggagctgg aagccgaaga ggcccctcgg ggcgaggccg ggggcgagga
    15541 agagcgtggt cgcgacggcg ccgacctcga cgatgaacat caccgggttc ttgatcatcc
    15601 gcgcggggcg gagcttcgcg aacgcgtccc gcgcggcccg ccggaggatc gcccggtcga
    15661 agagggaggt cgtttgcgta ttcgtcatgg cttcctcaga agagcttccc ggcgcccatc
    15721 aggaagtgct ccacgatcgg gccgagggag agggcgggaa agaacgtgag ggcgccgacg
    15781 acgacgacga cgctcgtgag gaggaagacg aacaccgggc cgtccaccgg gaaggttccg
    15841 gccgactccg cgacgcgctt cttggccgcg agggaccctg cgatgcccag cagcggcacg
    15901 atcatccaga aacggccgag gaacatcgcg agaccgagcg agacgtcgta gaaatccgag
    15961 ttcgcattca ggccggcgaa tgcgctcccg ttgtttccgg cggcgctcga gtacgcgtag
    16021 aggatctccg agaggccgtg cggccccgcg ttgttcaggc ccgcgaggcc cgctttcgtc
    16081 gcggcgccgg ccgccgaccc cacgaggatg cagaacgtca ggatgaggac ggagagcatg
    16141 gcgagcttca cctcgtgagc ctcgatgcgc ttgccgaggt actcgggcgt gcgcccgacc
    16201 atgagcccgg cgatgaagac cgagagcacc acgaagagga gcatcccgta gagccctgcg
    16261 ccgacgccgc cgaagacgac ctcgccgagc tggatgttca cgagggggac gaggccgccg
    16321 agcggcgtga aggagtcgtg catggagttc acggcgccgc acgacgcgtc ggtcgtgacg
    16381 gtcgcgaaga gggccgagtc gaccgggccg aagcggacct ccttgccttc catgttcccg
    16441 ggcgcctggc tcacgccgag ggcgtggtga gcggggttgc cgcgcgtttc cgcccagccg
    16501 gcaacgagag cgccgccggc gaagagaagc cccatcgccc agaagacggc ccacccgtgc
    16561 cgcggcttgc ccgtcagcct tccgagagtg acggtgaggc ccgccgggat cgcgaagatg
    16621 aggaccatct gcagaaggtt cgtgaacggc gtcgggttct cgaacggatg ggccgagttc
    16681 gcgttgaaga atccgcctcc gttcgtcccg agcatcttga tggcctcctg cgaggcgacc
    16741 ggcccgccgg ggatcttctg gatggcccct tccagcgtcg taagggaccg ggtcggctgg
    16801 aagttctgga tgacgccctg ggacatcagg acgactccgg cgacgaggct gatcgggagg
    16861 aggacccaca gcgtcgcccg gacgaggtct cgccagaaat tcccgatccc cctggagtcc
    16921 gcgcgggcga tgccgcgcac gaaggcaacg gcgatcgcca tgcccgtcgc cgccgagagg
    16981 aagttctgga cggcgaggcc cgccatctgc gtgagatggg acatcgtcgt ctcgcccgcg
    17041 taggcctgcc agttcgtgtt cgtcgtgaac gagacggccg tgttccacgc gagcgcgggc
    17101 gggaccgcgc cgagcctgtc ggggttgaag aagggcagga cgccctggag acgctggagc
    17161 gcgtacagga cgacgagcgc gacgacgttg aagacgagca tcgcggcgcc gtaggagcgc
    17221 cagccctggt cgcgggacgc gtcgacgccg gcgaggcggt agaccgcctt ctcgacggga
    17281 acgaggaccg ggtcgaggaa cgtcttcctg ccctcgaaga cgctcgccat gaacaccccg
    17341 aggggcttcg cggccgcgag gacgaggagg aagaagacgc cggtcttgag gaggacgtcg
    17401 ggcatgggca gctcacagct cctcgggatg aaggagcgca tagaggagat aggcgagggc
    17461 ggccgccgcg aggacgaggc cgaggaccgt ctcgaaggtc atggacgcct cacaggtcct
    17521 cgcagcgctt cgcgtaccag agcgtcgcgg cgaggagagc ggccgtgagg agcaggaaga
    17581 gggcgtcgag catgcggctg ttctcctaga acgtggcgac ggcgccgagg gcgaggagaa
    17641 actcttcgcg cttcgtgagc gggccgtccg ggcccccgaa agcgtcggcg gtggatctgt
    17701 cgtaccggcc ctcgagcttg agaatcacct gcgggtgggg gcgcagctcg aacgtgagcg
    17761 tcgagccgaa gagggtctga gagacgggag aaaccgcggc gtcggggtcg tggaagacct
    17821 cgacgcgcag ggcgagagcg agggagggag aggaagagga agacgaagat tttcttagaa
    17881 ggtattggag ccagaggctc gcgccctgcc agctggcggc tgcggcctgc gggcgagtct
    17941 gccgacccag atcgacgctc gcggcgatgt ttactttcga agaaatcttc agcgtcccga
    18001 cgaaatcgac gaacgaccgc caatgagcgt catcgttcgc cagctccggc ccgaagaacg
    18061 tgttcacggt caggccgagc gcgtccttcg cgtacgcgat ctgcgtcccg atcgccttgc
    18121 cgtcgttgtt gtcctcgatg ttctgccagc cgttcaggag ccagagctgg ccggaccagt
    18181 ggtcgtcgaa gctcgtcgcg actttgaggc cggtctggta gtacggcgag aactcgccca
    18241 tccagccccg cgtgtagttc cagttgtcct tcgagaagaa gccctcgtag ccgatgtggc
    18301 tgggctggat gccgagctcg aagaggacgc cgcgacccgc gtcgagcctg tacgacacgg
    18361 aggcctggga cacgtgtcgc caggtgtcca cgtggagctc ggcggcatgg acgacgtcgg
    18421 ttccggttcc ggcgacgccg acgatgtgga agccgacggg cttcgcgtcg agcgagacgt
    18481 ccagcgccgc gaggttcagg ccgaactggt tcgccttctc ggccgtcgtc cccgtgcccg
    18541 gctcgaagtt gttccgatcg gacggccggt tcgggttcca ggtcgcgtag gcgtcgacga
    18601 agccgtgcag cgtgaccgtc gaaccggcgg cgacgcctga ttcctgcggg ggcggtgtct
    18661 ggcccagggc catgtttcct tcgaagaaga gggcggcgag caccgcggcc gaggcggcgc
    18721 tgcgcgcgag ggcctcttcc cgcgcctttc gaaggccgtc ttctcttcgg acgttgcggc
    18781 ccttcgccac gcttggaagg tggcgcaagc gcgccaagga agtccggaac gagttctaag
    18841 aatgtcctaa agggcgttgc agtcgtcaga tcggctcggc gacgaagcgg tagccgaccc
    18901 agggctccgt gacgacgtaa cgggggcgcg cgggatccgg ctcgatcttc ttgcgcagcg
    18961 acccgacgtg gacgcgcacc gcgtctgggg tcgtgcccgg cgcgcctccc cacacccgtc
    19021 cgatgatctg gcggatcgtg acgggcttgc ccgcgtgtgc ggcgagcacc tcgaggatcg
    19081 agagctccgt cggggtgagc cgcacctcct tgccctcgac gaccacgcgg cggcgctcgc
    19141 ggtcgagggt gagccccggg aactcgagca ccttctccgc gctttcgtcg gagtggcggc
    19201 gcagctgcgc ccgcacgcgg gcgaggagct ccggcacgga gaaaggcttc acgacgaagt
    19261 cgtccgctcc gaggtcgagc gcgcggatct tgtcggcgtc gccgccgcgc acggaaagga
    19321 cgaggatggg cgtgttgccc gtttttcgga ggcgtgcgat cagctcgaag ccgtcgaccg
    19381 ccggcatcgc gaggtcggtc agaacgaggt ccggctcgcc ttcggcgaag cgacgggcgc
    19441 cttcctcccc gtcggcggcc tccagggtcg cgtaccccgc ggcctgaagc tccgcggcga
    19501 ggccgcgacg gatcgcgggg tcgtcgtcga cgacgaggat cgttgccaga gtccgcgtca
    19561 cgaggtcgcc tccggggcgg ccgggaggtc cagccgcgcg atcgtaccgc ccccggggcg
    19621 gtcgaggagc gcgaggctcc ccccgagagc gcgcgtgagc gacgccgaga tgacgaggcc
    19681 gaggccggcg ccgcccgtct cgcccgcgaa cacgccggaa gcggcgatgc gtttgacctc
    19741 ggccgatgca ccggggccgc ggtcccggac ttcgaatcgc acgcggctgc ggtcggtcgg
    19801 gtgcggctcg gcggcgagct cgagcgggac gcccgcgcgg gacgcccggg ccgcgttctc
    19861 gagaaggttc gtgaggatct cgacggcgag agtcgggtcc gcgagcgcgt cgggcgcgct
    19921 ggaagacacc gtgacgccca ccggccggcc cgcgaggacg agcggaaggg cctcgagggc
    19981 cgcgcggaag aggtcggcgg ggggcgcagg ctcgggcttc gggacgtacg tgcccgcgtc
    20041 gagccgcgcc atcgcgagca ggttgtcgat gcgccgggag aggcgctcgg tctccgccga
    20101 cacggcgtcg acgtcggccg tgtccgcggg cgaacgccgc agccgctcga ggccgaggcg
    20161 gagcgcggtg atgggcgtgc ggagatcgtg tgagacggcc cggaggaggg acgtcttgag
    20221 cgcgtcgctc tcgcgcaggg cctcgaggtg agttctctcc tcgaggaact tctcgcgctc
    20281 gacggcgagg gcgacgagcc ggccgacgga agcgagcgcg tgccggtcgg cgcgcgttcc
    20341 gcggacgagg aggacgccgg tcgggcgccc cccgagcgcg agcgggaggt acgcgtcgcg
    20401 ggcgccgtcg gcggacggca cctcggcggt ctctccgctt cggacgacgc tttcgagcag
    20461 cgggtccgag cggtcgaaac ggtggtcgcc ggtcgccacg acgacctcgc ctcccggcga
    20521 cgctgcagga gcgaggacga gagcaccgcc ccgggccccg atggacctca gggctcggcc
    20581 ggcggcgtcc ccgagggcgc cgacgcggtt cgtggccgcg aagaggtcga cggacagctc
    20641 gtagagcgct tccatttccg ttcgccgcgc ctcggcctcc tcggcgcggc gctgctcgtt
    20701 cgcgacgagc cggctcacga ggacggaggc gaggagaaag accgtcaggg cgacccagtt
    20761 cgccgggtcc gcgatcgtcc acgtcccgat cggcgggagg aagaagcggt tgaagcaggc
    20821 cgtggccgcg agcgacgcgg cgacggcggc ggcaaacccg agccgggcgg cggcgaagag
    20881 gacgacgacg aggtagacga agccgaccgt cgtcgcgttc gcgccgagct cgaggccggc
    20941 gatggtcgcc gcccccacgc cgaggagcgc gagcccgacg cggccgagag tcctacccgt
    21001 ggatgttgtg gccgtgaacg gcctccgcag cctcgtggag cgcctccgac agcgtcgggt
    21061 gcgcgtggat cgtccggatc atctcttcgg tggtcgcctc gaggcggagc atcgcggcgc
    21121 cctcggcgat gagctcggtc gcgtgcggcc cgacgatgtg gacgccgagg atctcgtcgt
    21181 acttcgcgtc ggagacgatc ttcacgaagc cttccggcga cggctccatc gccgtcttgg
    21241 cgaggacgcc gaacgggaac ttgcccgtct tgaccgcgta gccccgctcc ttcgccttcg
    21301 cctcggtgag cccaatcgat gcgacttcgg ggtcgcagta cgtgcagccg gggacctggt
    21361 cgcggttcac gggcaccgtc gggtggcccg ccatcgtctc ggcggccacg atgccctcgg
    21421 ccgacgccac gtgcgcgagc cagatcgtgg gggtgacgtc gccgatcgcc cacacccccg
    21481 gcacgttcgt gcgctggtgg gtgtcgaccc tcacgtaccc ccgctcgacc gcgacgccga
    21541 gcgcctcgag tccgaggccc tcggtgaccg gcttgcgtcc gaccgcgagg aggaccttct
    21601 cgacggacca cgagatcggc ccgccgtccg ggcctttcgc cgcgaccgtg acggagccgt
    21661 cgccgttcga cgtgacggac tcgacgccgg tcttcacgtg gacggcgatg cctttcttcc
    21721 tgaacgcctt ctcgatctcc ttcgacacct cttcgtcctc gacggggagg aggcgcggga
    21781 ggacctcgac gagaaccgtc ttcgagccgt aacgcgcgaa gcacgacgcg aactcggagc
    21841 ccacggcgcc ggagccgatc acgaggagcg acttcgggat cgacgtggac ttcaggatgt
    21901 ggtcggagtt caggatcgat acaccgtccg ctttgaggtg gggaaggtcc cgcgggacgg
    21961 agccggtcgc gatgaggacg ttcttcgtct cgagcgtcgt ctcgccggct tcgcccgtca
    22021 cggcgacgcg gttcgcagcg acgagtcgcc cgaagcccga gtgcacgtcg atcttgtgct
    22081 tcttcatgag gtattcgacg cccctcgcgc ctttgaagac gaccttgtct cggaacttca
    22141 cgacgccggg gtagtcgagc tcgacgccgg ccgtcctcac gccgaacgac gccgcgtggt
    22201 tcttcgtctc gtcgaagagc gacgcggact tcagcatggc cttggtcggg atgcagccgc
    22261 ggtggaggca cgtgcccccg aggccgacac cggcgacctt cgcgtccttc tcgacgatgg
    22321 cggtcttcag tccgagctgg gcggcgcgga tcgcggcgac gtagccgccg ggtcccgagc
    22381 cgatcacgac gaggtcgtac atctcactct ccttgatgag ggcgcacgcg gggcaggggc
    22441 ggtcgcgcgg cggggggatt ataggtggtc cgtcgagcgc gagaccgggg acgtagccgc
    22501 ctccgaacgc cctaagatgg tggcggtgat cggcgaggta ttcggacgct accggatcga
    22561 cgcgcggctc ggcgccggcg gcatggggga ggtctggcgc gcggaggaca tgcgcctcag
    22621 gcggcccgtg gccctcaagg tcctgcacgg cgcgcccacg gcggaggaat cggctcgcct
    22681 cctcagggag gcccgactcg cctcgcagct cagtcacccg aacatcgcgg tgatctacga
    22741 gatcgacgaa atcgagcgtg agggccggat ggtgcccttc attgcgatgg aatacgtcga
    22801 gggccgcacg ctggccgagc ggatcgcgga gggtcctctg gccgcggccg aggcgctgga
    22861 cgtcgcgtcc caggtggcgt ccgcgctcga ggccgcgcac gcgcgcggcg tggtccaccg
    22921 ggacgtgaag cccgggaacg tgatcgtcga cgcggcgggc cgcgtcaagg tcctcgactt
    22981 cgggctcgcc gcgtggcgcg cgccacgggg cttctcggga gagacgtgga gcgcgggacc
    23041 cgccgggccg acccagtcca tcccgggcgc ggtcgtcggg accatcgcgt acatgtcgcc
    23101 cgagcaggcg cgcggcgtcg acgtcgacgc gcgcacggac gtcttctcgc tcggcgtcct
    23161 cctctgggag actctcgcgg gccgccggcc gttcgaaggc ctgaacgccg tcgagatcct
    23221 cgaccgcgtg ctgcacgccg acccgccgcc gctttcccgc gcgtcggccg gcctcccgcc
    23281 gggtctcgaa tccgtcgtcg gccggatgct cgagaaggac cgcgagcggc gctacgcgac
    23341 gatgcgcgac gcgcgggtcg atctcgaggc cgtggcgcgc ggcgcggcgc ggacggcttc
    23401 gggcgcccag gcgccgggcg ggttcgcccc gccgtcgaac gcggtcgcgg tcgtcgcctt
    23461 ttcgaacatc acgaagagcc cggaggacga gtggcttcgg accggaatca tggagacggt
    23521 gacggcggat ctcaaggccg tcccgggact tgcggtgatc ggttgcgagc gcatctgcga
    23581 ggtggaaaaa cgcctgaccg gccaggcgtc gcacggtgag tcggagctcg cggcccgcct
    23641 gggacgcgag gtcggcgccc ggtgggtcgt gacgggcggc ttccagcgcc tcggggaccg
    23701 cgtgcggatc acggcgcgcg cgagcgagat cgagtccggc gaggtcctga cgaccgtgaa
    23761 ggtcgacggg gccatgggcg agctcttcga gctccaggac aggatcgtca cgcagctcgc
    23821 cgagggcttt cacgtcgccg cccgcccgtc ctcgggcgcg ggcgacgcga cgcacgtgat
    23881 cgacgcgtac gaggccttct ccaaggggct cgtgaacctc cgcgccgaga gccgcgaatc
    23941 ggtggaccgc gccatcctct tcttcgagcg ggccgtggcg ctcgacccga actacgcgcg
    24001 ggcctggatc gagctcggcg ccgcctacga cacgaaggcg acctacctcg ccatgcccga
    24061 gctcctcgaa cgggcggtgc aggcgttcga gcggggcctc gccctcgagc cgcggctccc
    24121 gcgggcatgg cgcgagctgg gcggcactct ggtcgctctc ggacgcgaag agcgcggctt
    24181 cgaggcgatc cgccgtgcgc tcgagctgga tcccgaggac gggagcgcgc acgccgctct
    24241 cggccgcgcg tacttcatcg ggaagggaat gttcccggag gccgcggcct ccttcgagcg
    24301 ggccctccag cggaatcccc agggcggctg gtacgcgctc cagctggcgc acgtggcgac
    24361 gctcctccgc gacttcccgc tcgccgatcg cgcggctcgg caggcgatcg agctccagga
    24421 gcgcggactc tcggggcgag agggtgttct gatcgtcggc gcctacatgc ggctcggcgt
    24481 cgctctcgcg ctgcagggtc ggtacgacga ggcgctcgaa gaatgccggc gggagctcgc
    24541 gttcctgagg cgcgtcgacc acgccctgaa ggagcgggtg atcgtcgagc tccacgtcca
    24601 catcggaagc gtgctcgtcg ccctgggccg ggcggagtcg ggccgggccg ctctccgcgt
    24661 cgcgctcgat gcttacgagg agcgcctgcg gatgggagcg gacgacccgt acacgcgcta
    24721 ctacgtggcc gcggcctggg cgctcctcgg ggagaaggag cgggcgctcg acgcgctcga
    24781 gcgcgccgcc tcgcagcgtc acgcctacac cgcggcgcga gccgtgatcg acccggactt
    24841 cgcgagcctc cgcgccgagg accggttcaa ggccctgacg gggggagaac caggaccccg
    24901 ggctgcctga cgtccgtcgc gatacgctcc cggcatgccg cctgaccttc tgagaaatct
    24961 tctcttcggc ttcgtcctcg tcctccctgc ctcgctcccg gccgccgagc ggcccgttcc
    25021 cggaaggctc cccacgggcg agacggtcct tccgagcggc cggctgctga cgccggcggg
    25081 gacgcagacc gaggtcgctc cctatccgtt cgcgctggcc ctgacgccgg acggaaggcg
    25141 ggtcgtcgcg gcgtgcaccg gggccgacaa ccagtcgctc catctcctgg atgcgtcgag
    25201 cgggaaggtc ctggccaagg agcccgtcaa gaagtcgtgg ctcggcctcg cggtgtcgcc
    25261 ggacgggacg cgcgtgtacc tcgcgggcgc gggcgggaag aacgtgctcg tctaccggct
    25321 ggagaacgac cgcttcaccg ccgagccccc cttgtccctg ctcgcacccg gcgagagtgc
    25381 caagcgcgac gcggcgccgt ccggactcgc cgcctccgcg gacgggaaga gcctctgggc
    25441 cgcgcggatc ctcctgaacg acgtcgtgag ggtcgacctc gcatcgtgca ccgtgacggc
    25501 ggtggtcccg gtcggcgtcc acccgtaccg acccgtgctg tcgccggacg ggtcgcttct
    25561 cgcggtcgcg aactggggcg cggcctccgt gtcgctcctg gacacatcct ccggtgcggc
    25621 cgtcgcgaac gtgaagacgc tcgaccaccc gtccgacctc gcgttctcac gcgacggccg
    25681 gacgctcttc gtggcccagt cgaaccggaa tctcgtcgcc gccgtggacc tcgcctcacg
    25741 cagcatcgtg cgtcagatct cggtggcact cgggccggac ggccccggca cgccgtctgc
    25801 ggatgcgctc ccggacggct cgaccccgaa cgcgctcgcc ctctctccgg acggcaggac
    25861 tcttttcgtc gcgaacgcgg acgacgacgc cgtcgccgtc gtggacgtgg gcggcgacgt
    25921 gcgcgcggcg cggacgaggg gattcatccc gagcgggtgg tacccggccg cgctcgcgct
    25981 ttcgcccgac ggcaggacgc tctgggtcgc gaacgcgaag ggcggctggt cgtgggccaa
    26041 cgccgcgggc gggccggatc ccacgcgaaa aggcgacgac aagatggcgt ggaagaagac
    26101 gcgcacgatc ccgggctccg tctcccgcgt cgacgtgccc tccgccagga cgctggccgc
    26161 gctcacggcc cggacctacg cgaaccgcag gccggcggcg cgcggggcgt ctcccgcgaa
    26221 ggcctcggcg gtggtcccgg cggcgcccgg cgctccctcc ccgatcaaac acgtcgtcta
    26281 cgtgatccgc gagaaccgga cgtacgacca ggtgctcggc gacctcccgc agggcaacgg
    26341 agacccgtcc ctcgtgctct tcgggcggga cgtgacgccg aacgcgcacg ctctcgccga
    26401 ggagttcgtc ctcctcgaca acctctactg cgacgccgag gtgtcggcgg acggccacaa
    26461 ctggtcgatg ggcgcgtacg ccacggactt cgtcgagaag gtctggccgc cggtctacgg
    26521 caacgtcgcg ttctcgtacc tcttcgaggg gaacgacgcg aacgccaccc cgacgaacgg
    26581 ctacctgtgg gacgcggccg cgcgcgccgg cctgaccctc cgcaactacg gggaattcgt
    26641 cggcacctcc gccgagtggt cgccgacggc gctgagcctc gaggaggggg cggtcggggc
    26701 cctgaaggga aacacctgtc ctttcttccc cgggttcgac accgacatcc tcgacaacgc
    26761 gcgggtggat gtattcctca aggagtttcg cggcttcgtg aaggcgaaag agatgccgcg
    26821 ccttctgatc ctgaggctcg ggaacgacca cacggtcggg acgaagaaag gcggacgtac
    26881 gccgcgcgcg atggtggccg agaacgacgt cgcgctcggc cgcctcgtgg aggcgatctc
    26941 gcactcgccc ttctggaagg agaccgcgat cttcgtgatc gaggacgacg cgcagaacgg
    27001 ctcggaccac gtcgacgcgc accggaccgt gggcttcgtg atctcgccgt acacgcgccg
    27061 gaagggcttc gtggactcga cgatgtactc cacggtctcg atgctccgga cgatggagct
    27121 gatcctcggc ctcccgccgc tgtcgcagca cgacgcctcc gccacgccga tgacgaacgc
    27181 gttcgcggac gcaccggacg acgtgccctt cgtccaccgc gacacgaaga tcccgttcta
    27241 cgagatgaac ccggacggcg cgcccatgca ggccgagtcg aacgggtggg acttcacgag
    27301 ggaggacgcg gcgcccgatc ttgcgctcaa cgaggcgatc tggaagagca ttcgcggggc
    27361 ggacgcggag atgcccgcgc ccgtgaacgc cgcgtgggtg cggctcgcga agacgagagg
    27421 ggactgacgg ctaccggagg cgctcgagcg gtacgtcgat cccgagggtg agcggcaaac
    27481 ggacgtgtac gtcccggttc tcgacgacga gcgtgtagat ccccggcgcc caggtcgcat
    27541 tcggagactc gaagtagacg tcgcccgacg cgagcgtccg cgagttcacc gcgaccttgt
    27601 cgaagacgat gtcctcgccg gggatcgcga agaaaccgag gcgctcctcc cggacggcgc
    27661 cggggaaata gccgttcagc gggtcgcgct gcaccttcgc ttcgttgagc atccggcgca
    27721 gatccgggat tccctcggcc atgcggcgct gcgacggcat cgggaggcgc ctgccgtcgg
    27781 gcatcacgag ccagacgtcc tctcgataga tctcgacggg cttcccgctc cagacggtca
    27841 gaccgatgtc gagcaggatc cacttccggt cgagatgggc ctgggcgtgg cggtatccga
    27901 cgacgatctg caccgagtcg tccctccagc gcatgaccgt cttgccgagg cgcgtgacgt
    27961 tcggcccgcc gtcgacgtcc gcggcgcccg gcgcggcgag gaagagaacg gcgaggccgg
    28021 cgattgcgag tctggggatg gggttcacgg aatcctccgg ggcccggagg ccctctcgag
    28081 acgtcccttg cacggagcat accgcccggg gaggccccct tttgcggacg gtcgccgcgg
    28141 cctcggagta cgattcccgg gccaaatgag tacgtcgtcg cttgtcgggg cgtggaagct
    28201 cctcgtcatc gaattccggt cggacgggca ggtcgtgtat cccgtgggac gcgacgcggc
    28261 gggaatgtgt ctttacgacg cctccgggca catgtctctt cagatcatgc gcgtcgaccg
    28321 gccgcgcttc gcgttgaacg accaccagaa cgggactccg gaggagatcg ccgcggcctt
    28381 caagggctac ctcgcgtacg gaggcaccta cgtcgaggat gccgaccacg gcgtcgtcgt
    28441 tcaccacgtc atgcactcgc tcttcccgaa ctggatcggc acggacctgg tccgcttctt
    28501 caagcgggcc gggaaccgcc tcatcgtgag cgcgccgacc cagaccgtcg ccgggcacct
    28561 gatggacgcg atgctcgtct gggagctcgt gtcggacgtc gaaaggacct gacgtgcagc
    28621 gcttcacgct ggcccgccac atgtcgcgcc gcctgacgct ctgccggcgc gtcgggccgt
    28681 gccccgccga cgcgcccgtg aggatcagcg tcctcctcca cgcgaaggac gggcgcgccc
    28741 tgcgcgaggc cgtgcacgcg gtctcggacc cgaagggcgc cgcgtacggc cgccacctca
    28801 cgccggacca ggtccgggac ctcgtcgcgc ccggatacca ggacctcgac gcggtcctct
    28861 cgtttctcga cgacgcggga ttgagggtcg agggcgtctc cggcgcgcgc acggccgtgc
    28921 aggcgagcgg tccggccgcg gcggtggc
//
LOCUS       DAOMFY010000018        28174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_542_length_28174_cov_6.877129, whole genome shotgun sequence.
ACCESSION   DAOMFY010000018 DAOMFY010000000
VERSION     DAOMFY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 28174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28174
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_542_length_28174_cov_6.877129"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLB01_03035"
     CDS             <1..806
                     /locus_tag="PLB01_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQQ76304.1"
                     /translation="AGTVVLRAVDVGQGDAWLLVTPRGRVLVDGGGSPDRAYEFGRLR
                     LIPRLADLGAVSLDAVVLTHPHPDHARGLLAVLDSLPVGRVLLPRAAPRNAFLDEFLA
                     VARKRRLVLERLGAGARFEAAGLAFDVLHPGDDRYPRARENNGSLVLRAKAEGRTVLL
                     TGDVESAAESDLVASGQDLRADVLKVPHHGSRTSTTPGFLSRVAPRVALVGVGRRNRF
                     GHPSSEVLARISAAGARVFRTDRDGDLALTLAAGRIFPRFPEAVARSAP"
     gene            803..1756
                     /gene="miaA"
                     /locus_tag="PLB01_03040"
     CDS             803..1756
                     /gene="miaA"
                     /locus_tag="PLB01_03040"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQQ76305.1"
                     /translation="MSGPSGVLAILGATATGKSELAVALAERLGGEVVSADAFAVYRG
                     FDVGTAKPGADLRARARHHLVDAREPVEPWSAGEFAAEARRLCEEILARGRLPILAGG
                     TGFYVRAFFGGLFEGPRRDAAVRSALEAVAARRGAGSLKRMVSVLDPEVASRLSDADA
                     SRAIRLLEILFLSGARPSRLFRERPGAAWARPSVKLLLTLPRADLHDRISERFRNRFA
                     TALPGEVQGLLAAGVPVTAPAFSAIGYRDTAALLEGAISEAEWKERILRDTRRYAKRQ
                     ETWFRREPGLVPIDATRTDLVDFAERLARPLLAHLPEGSHS"
     gene            1753..2016
                     /gene="hfq"
                     /locus_tag="PLB01_03045"
     CDS             1753..2016
                     /gene="hfq"
                     /locus_tag="PLB01_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HQQ76306.1"
                     /translation="MIGTPKPPINVQDGFFYQLRKDNTIVEVTLLSGRTRVGRIRRFD
                     KYAVVVEVDGREEMIYKHAIASVASTGANAPAFAPPAVRAPAV"
     gene            2013..3023
                     /locus_tag="PLB01_03050"
     CDS             2013..3023
                     /locus_tag="PLB01_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76307.1"
                     /translation="MTRRLAFALVAVLGSAAGCTTVPPPRDPAVVRGGEAASRAEAAA
                     AEGHLRDAVDGYREAIRLVPADARYVAREKELREAFVAKRSAEADEALKAKDTAAARR
                     AAVSILEVDPKAAAGYRALAKAAEAEGALEDAWTAAKRAHEIDPEDAALTESLAALAT
                     KTLRWAEAEALYEELGRTDPEFKEKAAAARFEFRVQNLPAPARRAALSPRLTRAQLAA
                     LLVELSPEVRDARIPPGAEVAVDVVDRPERAALVRTIGLGFFPVTRDTHLVGADAPVT
                     RSEMASHLRRLAALAGGELSCPASLAECGILPEGSRQLGGREAVAAIEAALKMARAGG
                     SR"
     gene            3020..3706
                     /locus_tag="PLB01_03055"
     CDS             3020..3706
                     /locus_tag="PLB01_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQQ76308.1"
                     /translation="MNVTELFSRVALFEGIAPEDRVALAKAATLRTYRRGETIVEQGQ
                     PGDAFYVIVKGRVAVAIVAPDGREVVLNSLGEGEHFGEMALLDDAPRSASVIAQEKSE
                     LAILSRPVFFGLLKSNFVLTRALLSAFSARLRRANATIEGLASLDVKARLARYFRELA
                     VARGRKAGGGWAVVVRPSQREIADTIGSSRETVSRTMSQMAAEQLIVPKGKAVYVRLE
                     GDGAAAPPPG"
     gene            3718..4308
                     /locus_tag="PLB01_03060"
     CDS             3718..4308
                     /locus_tag="PLB01_03060"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQQ76309.1"
                     /translation="MELRPWTLPNFLTFARLVALPFLVMAILDGREWTALVIFLTAAV
                     TDIVDGYLARRFAMGSPLGAWLDPIADKLFLLSSFVVFALPGTPTSVRIPIWLLVLTI
                     FRDVFILVICLVLFLALGIRSFPPSILGKLTTFLEISTVTAILLANVRRIDPVVATVC
                     MWLVAAFTIASGLHYSWRVLTQLPRGPHPPAAAPAA"
     gene            4305..4586
                     /locus_tag="PLB01_03065"
     CDS             4305..4586
                     /locus_tag="PLB01_03065"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQQ76310.1"
                     /translation="MSPLGRLFRRRSASPPALRLLTRPGCHLCDEMKATVGPLVARLG
                     GTIAEVDVDSDAALSERFGLEIPVLLDEEGRVIAKVRDSAERIARRLGA"
     gene            4608..6002
                     /locus_tag="PLB01_03070"
     CDS             4608..6002
                     /locus_tag="PLB01_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQQ76311.1"
                     /translation="MRFPVAALLLSGTLATPLLAERLPVQRHGVAEGLAEETVTALLK
                     DSRGYLWVGSLNGLSRFDGERFRVYGVGDGLPKARIFALAEAADGAVWVATSGGLARL
                     GASDPSTRPAFKPAGPAGGKPVEHVWADPKGRLWFGTGGEIFEGGRGDTPPRALGLAA
                     AAPGAVVRLIRAGAEGAILAGTSKGLFRATPGGKPERVPLSKESRQEDVRGLLIDHAG
                     RLWVATPGRLFVGARGWERSGAERAGPLLSNGRLDLPEAPGEWRVLDAIPGAGSSAWQ
                     RILELKDGRVVLTTTAGLVLVNGDRLEFSGRRNGLGDGILGELLEDGTGNLWIGTQST
                     GLLRLRPAGFTSFGEDDGLLDVQVADVFRGDGGALYVASRGNTTLARQEGKGFRSIRW
                     PRAAAPVLGRLIWGRSVVRDRSGAWWVAGPGGVARHPAAAFDESLARGKARTFGAAEG
                     VPGEVSALFEASAG"
     gene            6011..7666
                     /locus_tag="PLB01_03075"
     CDS             6011..7666
                     /locus_tag="PLB01_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ76312.1"
                     /translation="MTVTWAGVYDTPDSLLRFDAAAQRFAAVKPAGGLPAGAPLAFLE
                     DGKGGLWVGFGSGGAVRLTGGGADRLDPAHGAPEGFVHDFLLDGKGRLWVANGNAGLL
                     RVDDPAAATLAARPVDLASGAVPSSVLCLEGDAAGLLWVGTTRGVLRLDPATGRLAHF
                     TTADGLANNLVTSAARDASGALWFGTPEGVSRLVPGPDAASPPPRAVIAAFRVNGNPR
                     PVPELGTSALTGITLAPDENRVRVDFASPSFSPGGRVLFQTRLEGLDGQWSAPNADPT
                     VRYVGLAAGTYRLAVRAVGPDGAPGPEATAAFRVRPPLWKRPEFLAAGLALVALAAFL
                     VHRQGVKHAVAVERVRTRLATDLHDDVGSSLARISILSEVGRRDLDPSGETARLFSEI
                     GETSRGVIDALGDAIWSIDPKHDDLQSLADRLRHFATDLLEGRGIACRIEMPSGAGVV
                     DLPAEPRRHLFLLLKEAVTNAARHSGAKSVTVAFRLEDRKLSVEVADDGAGFDAPARN
                     AAETEGRGLENMRTRAVALGGTFTIAASPGGGTRVGVEGMPLP"
     gene            7663..8304
                     /locus_tag="PLB01_03080"
     CDS             7663..8304
                     /locus_tag="PLB01_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ76313.1"
                     /translation="MIRVAVVEDDSGLRESLRLLIGGTRGFAWAGGYRSAEEALQRLG
                     ATSGGLPDVVLMDIHLPGMPGSEAVRHVREAHPSVAVLMLSVYEGEDPIFASLCNGAA
                     GYLLKKTAPAKLLDAIRDVKEGGSPISPEIARKVIRAFRESVPAGRADHDLTPQEVRL
                     LKLLSDGASYQAAGDKLGISINTVRNYVRSIYEKLHVTSKSAAVSRGLKDRII"
     gene            8317..8712
                     /locus_tag="PLB01_03085"
     CDS             8317..8712
                     /locus_tag="PLB01_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76314.1"
                     /translation="MPTDPDDSPPLRSEVLVPPGEKPLGEAIRQTLQAAPRDRAALFE
                     RLAREIEAFVSATAPDHPWTCTVYIGTDGSRVFRGGVGHSLVIDADGRLWRARSYEDF
                     VTTYTISETTCSIESLTPLYGQMRQYLPR"
     gene            8759..9370
                     /locus_tag="PLB01_03090"
     CDS             8759..9370
                     /locus_tag="PLB01_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76315.1"
                     /translation="MRLRPAIVFSALMSSALVFAQAAPLREVNGDTLVSPADPVGSFV
                     FDKAFRYAGGQTIDVMKIAGAEQHFFVDAAPDRSIRRFYWIQFEHFHPDNTRTYDFSK
                     IPQKPVSIGRLAFAGDLRVKADYFTMDQRPGSDSKAAEDFLRARGFKLDGTFVTLRLF
                     HLPDDTKRSELMIIYGEALPAGASEERFKAEITAHAQANLKVP"
     gene            9415..10431
                     /locus_tag="PLB01_03095"
     CDS             9415..10431
                     /locus_tag="PLB01_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQQ76316.1"
                     /translation="MRAIVFTEYGSPDVLRLAEIERPVPADGEVLVRVHAASLNDWDH
                     GALQGADFVNRLLFGVLKPKKGRQVLGSDVAGRIEAVGRGVRRFRPGDDVFGDLSGRW
                     GGFAEFVCAPEEALALKAPGMTFEQAAAIPQAALLAVQALRDLGGIESGQRILVNGAG
                     GGVGTFALQIAALYDAEVTAVDAPEKLDMLRSMGARHVLDYTREDFTRAGERYDLILD
                     VKTTRSVLDCVRALNAGGTYVTVGGSMARLFQGLLLWPWIALTTRKKIRLLALKPNKD
                     LVFVRELFEAGKVVPVIDGHYGLADVPEAFRYFGSGRHKGKIVITVEQSDSASVAALL
                     PSRH"
     gene            10531..11403
                     /locus_tag="PLB01_03100"
     CDS             10531..11403
                     /locus_tag="PLB01_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ76317.1"
                     /translation="MKTTLGLVIDLVALSPPARAGSPEAVSGTATSPDGVPIRYDAAG
                     KGDPALVFVHCGGCNRGFWDGQMARFAAKHRVVALDLAGFGQSGRGRKDWTMDAFGQD
                     VVSVVEALGLKRVVLIGHCLGGPAVLEAARRMPGRVAGLVLVDSWVDFEGKIATEEME
                     KFLSALQADYRATTTAITSQYLFSASTPEPVKARVLETVLSVPQEIGVAAARSSMAYS
                     PLPALREIKAPVRAISSDLFPANPEGNRKYAPGYEAAIMKGAGHYLMLEQPEAFNALL
                     AEALRELTRSGGRR"
     gene            11465..11851
                     /locus_tag="PLB01_03105"
     CDS             11465..11851
                     /locus_tag="PLB01_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ76318.1"
                     /translation="MKRVTGIGGIFFKAKDAPALQAWYKRHLGIDVQEWGGTAFTWTD
                     GEGKPVAGTTIWSIGAAQGDPFAPSAATFMVNYRVEDLHGLVKVLREEGCNVLEKIDE
                     SEYGKFAWVIDPEGNKVELWQPPAGG"
     gene            11948..13108
                     /locus_tag="PLB01_03110"
     CDS             11948..13108
                     /locus_tag="PLB01_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ76319.1"
                     /translation="MNEGPDTPAGSAPARRSRLRVVLAVAVVVIVAAATARALVKTGG
                     RGGPRAEIVPVSAAAVAKKSIPLTTRAIGNVEAYATVAIRALVSGELVKIHFKQGQDV
                     AKGDLLFTIDPRPLQAAYDQAVGTLAKDKSTLANARALAARYEKLYNEGIASKEDWDQ
                     YRTAADTSAGLVAAGEAAVENAKVQLSYATIRSPISGMAGNLLVYEGNLVKANDTTPL
                     VTITQATPIYVTFAVPEGELAKVNRARAQGPVRVEAALPSDEARPVAGVLSFVDNAVD
                     STTGTIRLKATYDNPDRRLWPGQFVKVILTTGVEPDALVVPAQAIQTGQDGPYVYVVK
                     DDMTVEPRPVKPGPASEGVVAIAKGLSAGERVVTDGQLRLFPGAKVEIKTKG"
     gene            13105..16218
                     /locus_tag="PLB01_03115"
     CDS             13105..16218
                     /locus_tag="PLB01_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQQ76320.1"
                     /translation="MSITEPFIRRPVMTTLVMAGILLFGTIGFRSLPVSDLPNVDFPT
                     IQVTAGLPGASPETMAASVATPLERQFSAIAGLDSINSTSSQGTTQVTLQFALDRNID
                     AASQDVQTAIAAAARQLPTGMPSPPTLKKVNPADQPVLYLVLSSPTMPLTAVNEVAET
                     LIAQRLSTISGVAQVVVTGAQKPAVRVKLDPSQLAARGIGIDEVATSIQRQNSNLPVG
                     TLYGVHQSLTVKADGQLFDAAAYRTMIVAYRNGSPVTLGDLGKVVDGVENDRIGSWYD
                     DRQVVTLAIQRQPGSNTIAVVDAVKARLPALRAQLPAALKLDVLFDRSVPIRESVHDV
                     EFTLVLTIALVVLVIFLFLRNVRATLIPSLAVPASIVGTFAAMSLLNFSLDNLSLMAL
                     TLSVGFVVDDAIVMLENIVRRMEGGEDPLTAALKGGREIGFTILSMTISLVAVFIPVL
                     FMGGLLGRLFKEFAITISVAILVSGFVSLTLTPMLCSRFLKASHVANEGRFFKATERI
                     FDAWLALYERTLVWVLARQKETLIVSGILLLVTGILFAVIPKGFLTSDDTGQFIGYTE
                     GLPGVSFAQMGRHQKEVTDIIRSHPDVRGVSSTVGASDASPAGNSGSLLVLLKPRSER
                     KRDVDGVIEQLRPKLAAVSGIRVFLQNPPSVSIGGQVTRSPFQLTLQGSDRAQLAKYT
                     ELLETKVKTLPGLIDVTTDLQNRNPQLAVTIDRDKASALGLTASQVEDALNNAYGTRQ
                     VSTIYTSTNQYQVILELLEQYQTNPAALSLLYVRNTANTLVPLSSVARFDESAGPLAV
                     NHLGQVPSATISFNVKPGVALGDATSRVRKAADEILPLTIPATFQGTAEVFQSSVGGL
                     AFLLAIAILVIYLVLGILYESFIHPLTILSGLPSAGLGALVTLLIFGKDLNVYGFVGL
                     IMLVGIVEKNAIMMIDFAIESARTEGKGPAESIRRAAVVRFRPIMMTTMAALMGTLPI
                     ALGIGAGAESRQPLGLAVVGGLLVSQVLTLYLTPVVYIAFDSLQAKLTPEALPDLPED
                     AEPERAA"
     gene            16215..19349
                     /locus_tag="PLB01_03120"
     CDS             16215..19349
                     /locus_tag="PLB01_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQQ76321.1"
                     /translation="MSEKSLKPVQANVSALFIRRPVMTTLVMAGLFLFGAIGYVSLPV
                     ADLPKVDFPTINVGASLPGASPETMAAAVATPLERQFSGIAGIDSMNSTSSQGSTNIT
                     LQFSLDRDIDAAAQDVQTAIAATLSQLPPSMPNPPSLRKVNPAMQPILFIVLSSPTLP
                     MSEVSEWAETVLAQRISTVTGVAQVNVYGSKKYAVRVQVDPGVLASRQIGIDQVAAAI
                     SSGNSNRPVGVVYGPERALAIQTNGRLVNADAYKPLVVAWRNGAPVRLGEIANVLDSV
                     ENERQAAWFNGNRSIILAVQRQPDSNTIQVVDAVRALLPAFRMQIPAAMRMDVMFDRS
                     QGIRESVRDVQLTLVLTIVLVVFVIFLFLRNLRSTFIPSIALPTSLFGTFAVMAVLGF
                     SLDNLSLMGLTLSVGFVVDDAIVQLENIVRRMEKGESAAVASLRGSREIGFTILSMTV
                     SLVAVFIPVLFMGGLLGRLFQEFAVTISAAILVSGFVSLTLTPMLCSRLLKREEPGAA
                     RQGRVAAALESGFNRILKGYERTLHVVLEHRRITLATLVLMAVLAGFLFKLAPKGFIP
                     NEDIGMLRASVEGQQGISYQDMRDRQRAAAEIVRADPGVQSVQSSVGAGGPNSTANSG
                     SMFVTLKPRGQRDSSDAIVDRLRPKLAKVAGLRVFMQTPPAISIGGQHTRSLYQLTLQ
                     STDTEALYANAALLERKMRGIPIIRDVTSDLQIKNPQLDITIDRKKASALGITAGQIQ
                     RALALAYGSSQISTIYTATNEYEVILEVKPQDQRDASSLSSLYVRSGNGTLVPLTAIA
                     TARTTVGPLLVAHLGQLPSATISFNLGKDVALSQATAAVEKMARETLPGSITTSFQGT
                     AQVFQSSLKGMSILLVVAVLVIYLVLGVLYESFVHPLTILSGLPSAAVGAIFTLIIFG
                     KDLNVYAFFGLVMLIGIVKKNAIMMIDFALAAQRGEGMEPEKAIVTASLTRFRPILMT
                     TMAALAGTLPIALGLGRGGESRQPLGLAVIGGLVVSQVLTLYITPVVYLAFESLGARF
                     RARKTATAQEPAPA"
     gene            19571..20935
                     /locus_tag="PLB01_03125"
     CDS             19571..20935
                     /locus_tag="PLB01_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQQ76322.1"
                     /translation="MASALELARAWLDAERAYAQVPGVSAAIVHDQKVLWSGAFGLAD
                     VASGRKAEPGTIYSICSISKLFTSIGVMQLRDAGKLRLDDPVRSRLPWFTMKPGGTDG
                     TEITVEGLLTHAAGLPRETDLPYWTGPSFAFPTHEQIVERLAQQTALYPAATYIQYSN
                     LGLTLAGEIVAALSGISYADYVARNILVPLKLASTTPEMPESERGKRLASGYSALTRA
                     GRRDPVAFFQGKGVAPAMAFASTAEDLARFASWQFRLLGGGGPEVLKTATLREMHRVH
                     FVDPDFETMWGLGFEVRRSEKTTLVGHGGSCPGYRTHLMLQPDEKLAVVVLTNASGVD
                     AEGWAGLVREIVAPALKAAAKEAGKGKTPAPEMRKFAGTYSGAPWGGETFVLPWEDGL
                     AMLSLPSKNPMKDLIKMKKTGENTFRRIRKDDTLGEEIVFALGPDGKATRFTRHSNYS
                     TRME"
     gene            complement(20951..21394)
                     /locus_tag="PLB01_03130"
     CDS             complement(20951..21394)
                     /locus_tag="PLB01_03130"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HQQ76323.1"
                     /translation="MQTVAQDVALLRVTIPRSEIQKVMGSGLQEVMAAVVAQGAAVTG
                     PWFTHHLKMDPAVFDFEIGVPVSKPISPAGRVKPGTWPATRMARTVHKGGFEGLASGW
                     GRFDAWIKAEGLAPAGDLWERYLRGPESGLDPAEYRTEFNRPLAD"
     gene            complement(21407..21889)
                     /locus_tag="PLB01_03135"
                     /pseudo
     CDS             complement(21407..21889)
                     /locus_tag="PLB01_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            complement(21906..22283)
                     /locus_tag="PLB01_03140"
     CDS             complement(21906..22283)
                     /locus_tag="PLB01_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ76324.1"
                     /translation="MEPVGYSKSPAGLDATFFALADPTRRAILARLASGEASVSELAE
                     PFDMTQPAVSKHLRVLERAGLITRGRDAQRRPSRLEAKPLEEAVEWLENYRKFWERRF
                     QRLDALLDEMKAVQKPKRRRRPR"
     gene            complement(22337..22522)
                     /locus_tag="PLB01_03145"
     CDS             complement(22337..22522)
                     /locus_tag="PLB01_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HQQ76325.1"
                     /translation="MSEDFVVTCPYCGEDVEVILESDVRGSLVQDCEVCCNPWLLRVE
                     GEDEERTVSVSRADGSE"
     gene            complement(22519..23703)
                     /locus_tag="PLB01_03150"
     CDS             complement(22519..23703)
                     /locus_tag="PLB01_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQQ76326.1"
                     /translation="MAILRASALALLTVLPLHAFAGEPVTIGETFRIRSKVLNEDRTY
                     QVHLPASYPWAKDRRYPVLYVLDGRTHFRHMAASADFLAAEGEMPETIVVAVASTVRI
                     RDFTQTDWKEAWVGGGGAANFKRFLSTELIPLVERTYRTDGFRTLLGHSAGGQFVLYT
                     LTSEPALFRAYVALSPSLDWDHRLPARSLRKALESASSLSAFLYAARSDDSGQALADW
                     DELVAALGARKVPGFRWKTEAFPEERHRSIPLVGSIHALRALYDGYRMPEGLADGGIA
                     AVNRHYEAFSKTLGWPVAPTESAVNEVAYAELERGRTEEAIALFRRNVDAHPYSANAW
                     DGLKDGYAKVGRWKDAAAAADRALELARASDDPQLPEFEHQARKMRDRLNEETGPPRS
                     RE"
     gene            complement(23764..24228)
                     /locus_tag="PLB01_03155"
     CDS             complement(23764..24228)
                     /locus_tag="PLB01_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76327.1"
                     /translation="MNVRTLAGPTLAAVLALAASACFRSETPTGPSLPASNSGVVHVV
                     GQNVSPGALGLTRWATGETLGPVEVAPGGTVEMMRWNTRVQGTMFIVFVPIAEQPHFK
                     AVAQVEYNVVSVPASKADEISPVVRVSRDASGVVTATTDRPDVIEIGRVSPM"
     gene            complement(24360..24623)
                     /locus_tag="PLB01_03160"
     CDS             complement(24360..24623)
                     /locus_tag="PLB01_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76328.1"
                     /translation="MADGRPRVIAVECCVTGAFLLLAAAAVTGSPWLLVLGYAGHGFK
                     DWWQERSQFVSGTRWWPPFCAAVDFLVAAVLVVEIALGVGFHR"
     gene            complement(24767..26146)
                     /locus_tag="PLB01_03165"
     CDS             complement(24767..26146)
                     /locus_tag="PLB01_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQQ76329.1"
                     /translation="MSAQTLLIELSYFAASILFILGLRGLSHPDTARRGMFMAEFGML
                     LAVIGTLLHRDIVSYEWIIVGLLAGSTIGIAMGLWVPMTAMPQRTALSHAFGGLAAAL
                     VGVSEFLRHGEHLGTFKVTALGFEVLLGGITFTGSLVAFAKLQELVKGQPITYKGQNV
                     LNFLLFGSAVVIFIALVLNPGHTALFYAMCALALLFGVLLVLPIGAADMPVVIALLNS
                     YAGLASAATGFALGNNVLIIAGTLDGGSGFILSILMCKAMNRSITNVLFGAFGSATEA
                     AAGGEEGVMREVSLEDVATQLAYARQVVFVPGYGMATAQAQNAVRELASLLENRGVQV
                     KYAIHPVAGRMPGHMNVLLAEANVPYSQLYELEQINPEMPTTDVAVVIGANDVVNPEA
                     RDNPKSAIAGMPIIEVDKAKSVIVLKRGRGKGFSGLENPLFFKPVTGLLFGDAKTSLN
                     RLASAVGEL"
     gene            complement(26143..26448)
                     /locus_tag="PLB01_03170"
     CDS             complement(26143..26448)
                     /locus_tag="PLB01_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HQQ76330.1"
                     /translation="MNEVLIAGLYVFVLAAFLGYQVISRVPPLLHTPLMSATNAISGI
                     SLIGSLVTAGAKYNLTSTILGVIAVTAATINVVGGFIITDRMLKMFKREAKPAAEKK"
     gene            complement(26445..27605)
                     /locus_tag="PLB01_03175"
     CDS             complement(26445..27605)
                     /locus_tag="PLB01_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015198625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HQQ76331.1"
                     /translation="MKVSIPKEARADERRVALAPDTVKKLVASGLSVVVQSGAGLEAG
                     LPDADFTAAGASIEADRAALLAGADLLACVNRPEPADVARLRRGAAVIGFLRPLDEPE
                     GLRPFLDAGVTAFAMELVPRTTRAQAMDALSSMATIAGYKAVLVAAARLPKMFPLLMT
                     AAGTVPPAKVVVVGAGVAGLMAIATARRLGANVEAYDVRQAAGEEVRSLGARFLDVDL
                     GGIQTQDAGGYAVELSPEALKRGRDLVARHVKDADLVITTAQVPGRKAPLMLEEDAVR
                     GMKPGAFIVDLAAPTGGNTALTKPGEEVVVNGVTILAPLNLAASVPLHASQLYARNVA
                     AFLKLLVGEGGALTVNLNDDVVAGACAAHDGKPVNPRVAALLAPAAPTGAPR"
     gene            27710..>28174
                     /locus_tag="PLB01_03180"
     CDS             27710..>28174
                     /locus_tag="PLB01_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HQQ76332.1"
                     /translation="MSILEAAEVRSFVTSAAHPLPGGPAEYDPLLRAIGDARFVLLGE
                     ESHGTHEFARARVDVTMRLIREKGFRGVAVEADGPDAARVDRWVRGGGDEADGADALA
                     GFRRFPSWPWRNADVLDFVEWLRAHNEAIPAGRPKAGFWGLDLYSLHASMEEA"
BASE COUNT         4075 a         9908 c         9904 g         4287 t
ORIGIN      
        1 acgccgggac cgtcgtcctc cgggcggtgg acgtcgggca gggagacgcg tggctgctcg
       61 tgacgccgcg cgggcgcgtg ctcgtggacg ggggcgggag ccccgaccgg gcgtacgagt
      121 tcggccggct gcggctgatc ccacggctcg cggacctcgg cgccgtgtcg ctcgacgcgg
      181 tcgtcctcac ccacccgcac ccggaccacg cgcgcggcct gctcgccgtc ctggactccc
      241 tccccgtggg ccgcgtcctc ctcccgcgcg ccgcgccgcg aaacgcgttc ctcgacgagt
      301 tcctcgccgt cgcgcggaag cggcgcctcg tcctcgagcg gctgggcgcg ggcgcccgct
      361 tcgaggccgc aggcctcgct ttcgacgtcc tgcacccggg agacgaccgg tacccgcgcg
      421 cccgggagaa caacggatcg ctcgtcctcc gggcgaaggc cgaggggcgg acggtcctcc
      481 tgacgggcga cgtcgaatcg gccgccgaga gcgacctcgt ggcctccggg caggatctcc
      541 gggccgacgt cctgaaggtg ccgcaccacg gaagccgcac ctcgacgacg ccgggctttc
      601 tctctcgtgt cgctccgcgt gtcgctctcg tcggcgtcgg ccgccgcaac cggttcgggc
      661 atccctcgtc cgaggtgctc gcgcggatct cggcggccgg ggcgcgcgtc ttccggacgg
      721 accgcgacgg cgacctcgcc ctgacgctgg ccgcgggccg catctttccc cggttcccgg
      781 aggccgtcgc gaggtccgcg ccgtgagcgg cccgtccggc gttctcgcga tcctcggcgc
      841 gacggcgacg gggaagtccg agctcgcggt ggcgctcgcc gagcgcctcg ggggagaggt
      901 cgtctcggcg gacgcgttcg ccgtgtaccg cggcttcgac gtggggacgg ccaagcccgg
      961 cgcggacctc cgcgcgcgcg cgcggcacca tctcgtggac gcgcgggagc ccgtcgagcc
     1021 gtggagcgcg ggagagttcg ccgcggaggc gcggcgtctc tgcgaggaga tcctcgcgcg
     1081 cggccgcctg ccgatcctcg cgggaggcac cggcttctac gtccgcgcgt tcttcggcgg
     1141 tctcttcgag gggccgcggc gggacgccgc cgtccgctcc gcgctcgagg ccgtcgcggc
     1201 ccgccggggc gcggggtccc tcaagcggat ggtctccgtt ctcgaccccg aggtcgcctc
     1261 gcgcctctcg gacgccgacg cgtcgcgcgc gatccgcctc ctcgagatcc tcttcctctc
     1321 gggcgcgcgt ccgtcgcggc tcttccgcga gcgccccggc gcggcgtggg ctcggccttc
     1381 cgtgaagctg ctcctcacgt tgccgcgcgc ggacctccat gacagaatct cggagagatt
     1441 tcgaaaccgc ttcgcgaccg cgctccccgg ggaagtgcag ggcctgctcg ccgccggcgt
     1501 gccggtcacg gcgcccgcgt tctcggcaat cgggtatcgt gatacggctg cgcttctcga
     1561 gggggcgatc tccgaggcgg agtggaagga acggatcctg agagacacgc gccgctacgc
     1621 gaagagacag gagacctggt tccggcggga accgggtctc gtgccgatcg acgcgacgcg
     1681 taccgacctc gtcgatttcg cggagcgcct cgcgcgcccg cttcttgctc atcttccgga
     1741 agggagtcat tcatgatcgg gacgccgaag ccgcccatca acgtccagga cgggttcttc
     1801 taccagctcc gaaaggacaa cacgatcgtc gaggtgacgc tcctctccgg ccgcacgcgc
     1861 gtcgggcgga tccgccgttt cgacaagtac gccgtcgtcg tcgaagtcga cgggcgcgaa
     1921 gagatgatct acaagcacgc gatcgcgtcg gtggcgtcga ccggtgccaa cgctcccgcc
     1981 ttcgcgcctc cggcggtgcg ggcacccgcg gtttgacgcg ccggctcgcg ttcgccctgg
     2041 tggccgtcct cgggtcggcc gcggggtgca cgacggtacc gccccccagg gacccggccg
     2101 tcgtgcgcgg cggtgaagcc gcctcgcgcg ccgaggccgc agcggccgag ggccacctgc
     2161 gcgacgccgt ggacggctac cgggaggcga tccggctcgt cccggcggac gcgcggtacg
     2221 tcgcgcgcga aaaggaactc cgggaggcct tcgtcgcgaa gcgctcggcc gaggcggacg
     2281 aggccctgaa ggcgaaggac accgcggcgg cccggcgcgc cgccgtctcg atcctcgagg
     2341 tcgacccgaa ggcggcagcg ggctatcggg ctctcgcaaa ggctgccgag gccgagggcg
     2401 cgctcgagga cgcctggacc gcggcgaagc gggcgcacga gatcgacccg gaagacgctg
     2461 ccctgacgga gtccctcgcc gccctcgcga cgaagacgtt gcgctgggcc gaggccgagg
     2521 ccctttacga ggagctcggc cggacggacc cggaattcaa ggaaaaggcg gccgcggcgc
     2581 gcttcgagtt ccgcgtccag aacctgccgg ctccggcgcg gcgggcggcg ctctctccgc
     2641 ggctcacgcg ggcgcagctc gccgcgcttc tcgtcgagct ctcgccggag gtccgggacg
     2701 cgcgcatccc gccgggagcg gaagtcgcgg tggacgtcgt ggaccggccc gagcgcgcgg
     2761 cactcgtgag gacgatcgga ctcggcttct tccccgtcac gcgggacacg catctcgtcg
     2821 gggcggacgc gcccgtcacg cggagcgaga tggcgtctca tctcaggagg ctcgcggcgc
     2881 tcgcgggagg cgagctctcg tgcccggcgt ccctggccga gtgcggcata cttccggagg
     2941 ggtcccgtca gctcggcggg cgtgaagcgg tcgccgcgat cgaggcggcg ctgaagatgg
     3001 cccgggcagg aggatcgcga tgaacgtcac ggagctcttc tcgagagtcg ccctgttcga
     3061 agggatcgcc cccgaggacc gtgtcgcgct cgcgaaggcc gcgaccctcc gcacctaccg
     3121 ccgcggcgag acgatcgtcg agcagggtca gcccggcgac gcgttctacg tgatcgtgaa
     3181 gggccgcgtc gcggtcgcga tcgtcgcgcc ggacgggcgc gaggtcgtcc tgaactcgct
     3241 cggcgagggc gagcacttcg gcgagatggc gctcctcgac gacgccccgc gctcggcgag
     3301 cgtgatcgcg caggagaagt ccgagctcgc gatcctctcg cgcccggtgt tcttcggcct
     3361 tctgaagtcc aacttcgtcc tcacgcgcgc gctcctctcc gcgttctcgg cgcgcctgag
     3421 gcgcgcgaac gccacgatcg agggactcgc ctcgctggac gtcaaggcgc gcctcgcgcg
     3481 ctacttccgc gagctcgccg tggcgcgggg gcggaaggcc ggcggcggct gggccgtcgt
     3541 cgtgcgcccg tcccagcgcg agatcgccga cacgatcggc tcctcgcgcg agacggtctc
     3601 gcgcacgatg tcccagatgg ccgccgagca gctcatcgtc ccgaagggca aggccgtcta
     3661 cgtgcggctc gagggcgacg gcgcggcggc tccgccgccc ggctgacggg acgcgccgtg
     3721 gagcttcgcc cctggacgct gccgaacttc ctcacgttcg cgcgcctcgt cgcgctcccg
     3781 ttcctcgtca tggcgatcct cgacggccgc gagtggacgg cgctcgtcat cttcctcacg
     3841 gcggccgtca ccgacatcgt ggacgggtac ctcgcgcgcc gtttcgcgat ggggtcgccg
     3901 ctcggcgcgt ggctcgaccc gatcgccgac aagctcttcc tcctctcgtc gttcgtcgtc
     3961 ttcgccctgc cggggacgcc gacgtccgtg cgaatcccga tctggcttct cgtcctgacg
     4021 atcttccggg acgtcttcat cctcgtgatc tgcctcgtcc tgttcctggc cctcgggatc
     4081 cgttcgttcc cgccgtcgat cctcggcaag ctcacgacgt tcctcgagat ctcgaccgtg
     4141 accgcgatcc tgctcgcgaa cgtgcgccgc atcgaccccg tcgtggcgac ggtctgcatg
     4201 tggctcgtcg cggcgttcac gatcgcgtcg gggcttcact actcgtggcg cgtcctcacg
     4261 cagctgcctc gcggccctca cccgccggct gccgcgcctg ccgcgtgagc cctctcggcc
     4321 gcctcttccg gcgccgcagc gcgtcgccgc cggcgcttcg cctcctgacg cgtccgggct
     4381 gccacctctg cgacgaaatg aaggcgacgg tcgggccgct cgtcgcccgg ctcggcggca
     4441 cgatcgccga ggtggacgtc gactcggacg cggcgctctc ggagcgattc ggcctcgaga
     4501 tccccgtcct cctcgacgag gagggccgcg tcatcgccaa ggtccgcgac tccgccgagc
     4561 ggatcgcgag gcgcctcggc gcctgagcgc tcagaatgcg ggggacgatg cgattcccgg
     4621 ttgccgccct tctcctctcc ggcaccctcg cgacgccgct cctcgccgag cgtctgccgg
     4681 tccagcggca cggcgtcgcc gagggcctcg cggaggagac ggtgacggcc ctcctcaagg
     4741 actcgcgcgg ctacctctgg gtcggctcgc tgaacggcct ctcccggttc gacggcgagc
     4801 gtttccgcgt ctacggcgtc ggggacggcc tcccgaaggc gcgcatcttc gccctcgcgg
     4861 aagccgcgga cggtgcggtc tgggtcgcca cgtcgggcgg cctcgcgcgg ctcggcgcgt
     4921 ccgacccttc cacgcggccc gcgttcaagc cggcggggcc ggcgggagga aagcccgtcg
     4981 agcatgtctg ggcggatccg aaagggcgcc tgtggtttgg cacgggggga gagatcttcg
     5041 aaggtggaag aggggacacg ccgccgcgcg cgttgggcct cgccgccgcg gcgcccggcg
     5101 ccgtcgtccg cctcatccgg gcgggcgccg aaggggcgat cctggccggc acgtcgaagg
     5161 gcctctttcg cgccacgccc ggcggcaagc cggagcgtgt cccgctttcg aaggaatccc
     5221 ggcaggagga cgtgcgcggt ctgctgatcg atcatgcggg gcggctttgg gtcgcgaccc
     5281 cgggccgcct cttcgtcggc gcccgcggat gggagcgcag cggagccgag agggccggac
     5341 ccctcctgtc caacgggcgg ctcgacctcc ccgaggcacc cggggagtgg cgggtcctcg
     5401 acgcgattcc gggggcaggc tcgtcggcct ggcagcggat cctcgaactc aaggacgggc
     5461 gcgtcgtcct cacgaccacg gcgggcctcg tgctcgtgaa cggcgaccgc ctcgagttct
     5521 ccgggcggcg aaacggtctt ggggacggaa tcctcggcga gcttctcgag gacggcaccg
     5581 gcaacctctg gatcgggacg cagagcacgg gccttctgcg ccttcgcccc gcggggttca
     5641 cgtccttcgg cgaggacgac ggactcctcg atgtccaggt cgccgacgtg tttcggggcg
     5701 acggcggcgc gctctacgtc gcgtcgcgcg gcaacacgac gctcgcgcgc caggagggca
     5761 agggcttccg ttcgatccgg tggccgcgcg cggcggctcc cgtcctcggc cgcctcatct
     5821 ggggccgatc ggtcgtgcgc gaccggtccg gcgcatggtg ggtcgccggg ccgggcggcg
     5881 tcgcgcggca cccggcagcc gcgttcgacg agtcccttgc gcgcgggaag gcgcggacgt
     5941 tcggcgctgc cgagggcgtt ccgggcgagg tctccgcgct cttcgaggcc tcggctggct
     6001 gatcttcctg gtgacggtca cctgggccgg cgtctacgac acgccggact cgctcctgag
     6061 gttcgacgcg gcggcgcagc gcttcgccgc cgtgaagccg gcgggcggtc tgccggccgg
     6121 cgccccgctc gcgtttctcg aagacggaaa gggcgggctc tgggtgggat tcgggagcgg
     6181 aggggcggtc cgcctgacgg gcggcggcgc cgaccggctc gaccccgcgc acggcgcccc
     6241 ggaaggcttc gtccatgact tcctcctcga cggcaagggc cgcctctggg tcgcgaacgg
     6301 caacgcgggc ctcctgaggg tggacgatcc cgcggccgcg acgctcgcgg cccgtcccgt
     6361 ggacctcgcg agcggggcgg tgccgagcag cgtgctctgc ctcgagggcg acgcggcggg
     6421 tctcctctgg gtcgggacga cgcgcggcgt cctccgcctc gatcccgcga ccgggcggct
     6481 ggcccacttc acgacggcgg acggcctcgc gaacaacctc gtgacgtcgg cggcgcgcga
     6541 cgcatccggc gccctgtggt tcggaacgcc cgagggcgtc tcgcgcctcg tgcccggacc
     6601 cgacgcggcg tcgccccctc cacgcgcggt gatcgcggcg ttccgcgtga acggaaaccc
     6661 ccggcccgtg cccgagctcg gaacgtccgc gctcaccggg atcacgctcg ccccggacga
     6721 gaaccgcgtc cgcgtggact tcgcgtctcc gagcttctcg ccgggcggcc gcgtcctctt
     6781 ccagacgcga ctcgagggcc tggacgggca gtggagcgcg ccgaacgccg acccgacggt
     6841 ccgctacgtg ggcctcgcgg ccggcacgta ccgcctcgcc gtccgggcgg tcgggccaga
     6901 cggggcgccg ggcccggaag ccaccgcagc gttccgcgtg cggccgccgc tctggaaaag
     6961 gcccgagttc ctcgcggccg gcctcgcgct cgtcgccctc gcggcgttcc tcgtccaccg
     7021 gcagggcgtg aagcacgccg tcgcggtcga gcgcgtccgc acgcgcctcg cgacggacct
     7081 ccacgacgac gtcggctcga gcctcgcgcg catctcgatc ctctcggagg tcggccgccg
     7141 cgacctcgac ccctccggcg agacggcccg cctcttctcc gagatcggcg agacgtcgcg
     7201 cggcgtcatc gacgcgctcg gcgacgcgat ctggtccatc gaccccaagc acgacgacct
     7261 tcagagcctc gcggaccggc tccgccactt cgcgacggac cttctcgaag gacgcggcat
     7321 cgcgtgccgc atcgagatgc cgtccggcgc cggcgtcgtc gacctgccgg ccgagccgcg
     7381 gcgccacctc ttccttctcc tcaaggaggc cgtcacgaac gccgcgcgcc actcgggcgc
     7441 gaagtccgtc accgtcgcgt tccgcctcga ggaccggaag ctctccgtgg aagtcgccga
     7501 cgacggcgcc ggcttcgacg cgccggcgcg gaacgccgcc gagaccgagg ggcggggtct
     7561 cgagaacatg cggacgcgcg ccgtggccct cggaggaacg ttcacgatcg ccgcgtcccc
     7621 gggcggcggg acgcgcgtcg gcgtcgaagg gatgccgctg ccatgatccg cgtcgccgtc
     7681 gtcgaggacg actccggtct gcgcgagagc ctgcgcctcc tgatcggcgg gacgcgcggg
     7741 ttcgcgtggg ccggcgggta ccgctccgcc gaggaggctc ttcagcgcct cggcgcgacg
     7801 tcgggcgggc tgccggacgt cgtcctcatg gacatccatc tgccgggcat gccgggctcg
     7861 gaggccgtgc ggcacgtgcg cgaggcgcac ccgtcggtcg cggtcctcat gttgagcgtc
     7921 tacgagggcg aggatccgat cttcgcgtcc ctctgcaacg gcgcggccgg ctacctcctg
     7981 aagaagaccg ctcccgcgaa actcctcgac gcgatccgcg acgtgaagga gggcggttcg
     8041 ccgatctcgc ctgagatcgc gcggaaggtg atccgggctt tccgcgagtc cgtgcccgcg
     8101 gggcgcgccg accacgacct cacgccgcag gaggtgcggc tcctgaagct gctctcggac
     8161 ggcgcgagct accaggcggc gggggacaag ctcggcatca gcatcaacac cgtacggaac
     8221 tacgtgcgct ccatctacga gaagctccac gtgacgtcca agtctgcggc ggtgagccgc
     8281 gggctgaagg accggatcat ttgaaggtcg cgggacatgc cgacggaccc tgacgactcg
     8341 ccgcccctcc ggtcggaagt cctcgtcccg cccggcgaga agcctctcgg cgaggcgatt
     8401 cgtcagacgc ttcaggctgc gccccgggat cgggccgcgc ttttcgagcg gctcgcccgt
     8461 gagatcgagg ccttcgtctc ggccacggct cccgatcacc cgtggacctg caccgtctac
     8521 atcggcacgg acgggtcgcg tgtctttcgg gggggcgtcg ggcactccct cgtcatcgac
     8581 gcggatggcc gcctgtggcg cgcgcgcagc tacgaggact tcgtcacgac gtacacgatc
     8641 tcggagacga cctgctcgat cgagtcgctc acgccgctct acggacagat gaggcagtac
     8701 cttccccgct agagcgaaac ccccgcgggc gccgggcgta ctgttcgtgg aggaatccat
     8761 gcggcttcgc cctgcgatcg tgttttctgc gctgatgtcg tctgctctcg tcttcgcgca
     8821 ggccgccccg ctgcgcgagg taaacggaga cacgctggtc tccccggccg accccgtcgg
     8881 gtcattcgtc ttcgacaagg cgtttcgata cgccggcggc cagacgatcg acgtcatgaa
     8941 gatcgcgggc gccgagcagc atttcttcgt cgacgccgcg cccgaccgct ccatccgccg
     9001 cttctactgg atccagttcg agcacttcca tccggacaac acgcgcacct acgacttctc
     9061 gaagatcccg cagaagcccg tctcgatcgg gcggctcgcg ttcgcgggcg acttgagggt
     9121 taaggcggac tacttcacga tggaccagcg tcccggctcc gactcgaagg ccgccgagga
     9181 cttcctgcgc gcccgcggct tcaagctcga cggcacgttc gtcacgctgc gcctcttcca
     9241 cctcccggac gacacgaagc ggagcgaact catgatcatc tacggcgagg ccctcccggc
     9301 cggtgcgtcg gaggaacggt tcaaggcgga gatcaccgcg cacgcgcagg cgaacctgaa
     9361 ggtgccttga cgcgcccggc gagggcgtga cacggtgccg gtcatggaga ccgcttgagg
     9421 gcgatcgtct tcacggagta cggatcgccg gacgttctgc ggctcgcgga gatcgaacgc
     9481 cccgttcccg cggatggcga ggttcttgtc agagtccacg ccgcttcgct gaacgactgg
     9541 gaccatggcg ccctccaggg agcggacttc gtgaaccgcc tcctgttcgg cgtcctcaag
     9601 ccgaagaagg gaaggcaggt cctcggctcc gacgtcgcgg gacgcatcga ggccgtgggg
     9661 cggggcgtgc ggcggttccg tcccggcgac gacgtgttcg gagacctcag cggcaggtgg
     9721 ggcggattcg cggaattcgt gtgcgctccc gaggaggcgc tggcgctgaa ggcgcccggc
     9781 atgacgttcg aacaggcggc cgcgatcccc caggcggcgc tgctcgccgt gcaggccctt
     9841 cgcgatctcg ggggaatcga gtccggacag aggatcctcg tcaacggcgc cggcggcggc
     9901 gtggggacgt tcgcgctcca gatcgctgcg ctctacgacg ccgaggtgac ggccgtggac
     9961 gcccccgaga agctcgacat gctgcgctcg atgggcgcac ggcacgtcct cgactacacg
    10021 cgggaagact tcacccgggc gggggagcgc tacgacctga tcctggacgt caagacgacc
    10081 cgatccgtcc tcgactgcgt gcgggccctg aacgcaggcg ggacctacgt tacggtcggc
    10141 ggttcgatgg cccggctctt ccagggcctg ctgctctggc cctggatcgc gctcacgacg
    10201 cgcaagaaga tccgtctcct ggcgctgaag ccgaacaagg accttgtgtt cgtgcgggag
    10261 ctgttcgaag cggggaaggt cgtccccgtg atcgacgggc actacggtct cgccgacgtg
    10321 cccgaggcgt tccggtactt cggatcgggc cgccacaagg ggaagatcgt gatcacggtg
    10381 gaacagtcgg actcggcgtc agttgctgcg ctccttcctt ctcgccactg agtcttgata
    10441 tcgttagccg accagacgcg gatgtgggtg tgcgcgagcc tcgggaagtg cggcctcctg
    10501 cggtgacacg ctgaggcgaa ggaggattcg atgaagacca cgctcggcct cgtcatcgac
    10561 ctcgtcgcac tgtccccgcc ggccagggcc ggctccccgg aggccgtttc gggcaccgcc
    10621 acctcgcccg acggcgtccc catccgctat gacgcggccg gcaagggcga tccggccctg
    10681 gtgttcgtcc actgcggggg ctgcaaccgg ggcttctggg acgggcagat ggcccgcttc
    10741 gccgcgaagc accgcgtcgt cgcgctcgac ctggccggct tcggccagag cggacgcggg
    10801 cgcaaagact ggacgatgga cgctttcggc caggacgtgg tgtcggtggt cgaggcgctg
    10861 ggcttgaagc gggtggtgct catcggccac tgtcttggcg ggccggcggt cctggaggcc
    10921 gcgcgccgga tgccgggccg ggtggccggc ctggtgctgg tggacagctg ggtggacttc
    10981 gagggaaaga tcgcgaccga ggagatggag aagttcctgt cggcgctcca ggccgactac
    11041 cgggccacga caaccgccat cacgtcgcaa tacctcttct cagcgagcac gcccgagccc
    11101 gtcaaggcgc gcgttctgga aacggtgctg tcggtgccgc aggagatcgg cgtggccgcc
    11161 gcccgctcca gcatggcata cagcccgctg ccggcgctgc gcgagatcaa ggcccccgtc
    11221 cgcgccatca gctccgacct gtttcccgcc aacccggaag gcaaccgcaa gtatgccccc
    11281 ggctacgagg cggcgatcat gaagggagcg gggcattacc tcatgctcga gcagcccgag
    11341 gccttcaacg cgctcctggc ggaggccctg cgcgagctga cccggagcgg cgggcggcgg
    11401 tgaggtttcg ggtagctgtt gacctggtga atggcagagt gttgagcacc tcctcggaga
    11461 ttccatgaag cgagtcacag gcatcggcgg catcttcttc aaggccaagg acgctccggc
    11521 gctgcaggcc tggtacaagc ggcaccttgg aatcgacgtc caggaatggg gcggcacggc
    11581 tttcacctgg accgacggcg aaggcaagcc ggttgcggga acgaccatct ggtccattgg
    11641 tgcggcgcag ggcgacccgt ttgcgcccag cgccgcgacg ttcatggtga attaccgggt
    11701 ggaggatctc cacggcctgg tcaaggtcct gcgcgaagaa ggctgcaacg ttctcgagaa
    11761 gatcgacgaa tccgagtacg gcaagttcgc ctgggtcatc gacccggaag gaaacaaagt
    11821 cgagctctgg cagccgcctg ccggcgggtg atcggggcag tccgggggcg catgtccatg
    11881 ctctgagcgg acctcctcgc gaagcccaga attccgggct ggcgcttcct gcgccccggg
    11941 aggttccatg aacgagggac cggacacgcc ggccgggtcc gccccggcga ggcggagccg
    12001 cctgagggtc gtcctcgccg tcgcggtcgt cgtgatcgtc gccgcggcca cggcgcgcgc
    12061 gctcgtgaag acgggcggcc ggggcggccc gcgagccgag atcgtgcccg tctcggcggc
    12121 cgccgtcgcg aaaaagtcga ttccgctcac gacgcgggcg atcgggaacg tcgaggcgta
    12181 cgccacggtg gcgatccgcg cgctcgtctc gggcgagctc gtcaagatcc acttcaagca
    12241 gggccaggac gtggcgaagg gcgaccttct cttcacgatc gacccgcgcc cgctgcaggc
    12301 ggcttacgac caggccgtcg gcaccctcgc gaaggacaag tcgacgctcg cgaacgcgcg
    12361 cgcgctcgcg gcgcggtacg agaagctcta caacgagggg atcgcctcca aggaagactg
    12421 ggaccagtac cgcacggccg ccgacacgtc cgcggggctc gtcgcggccg gcgaggccgc
    12481 ggtcgagaac gcgaaggtcc agctctcgta cgcgacgatc cgttcgccga tcagcgggat
    12541 ggcgggcaac ctcctcgtct acgagggcaa cctcgtgaag gcgaacgaca cgacgcccct
    12601 cgtcacgatc acgcaggcga cgccgatcta cgtgacgttc gccgtgcccg agggcgagct
    12661 cgccaaggtc aaccgcgcgc gcgcccaggg cccggtgcgc gtcgaggccg cgctcccgag
    12721 cgacgaggcc cgtcccgtcg ccggggtcct ctcgttcgtg gacaacgccg tcgactccac
    12781 gacgggcacg atccgcctca aggccacgta cgacaacccc gaccgccggc tctggcccgg
    12841 gcagttcgtg aaagtgatcc tcacgacggg cgtcgagccc gacgcgctcg tcgtcccggc
    12901 ccaggcgatc cagaccggcc aggacggtcc gtacgtctac gtcgtgaagg acgacatgac
    12961 cgtcgagccg cgccccgtga agcccgggcc cgcctctgag ggcgtcgtcg cgatcgccaa
    13021 ggggctctcg gcgggcgagc gcgtcgtcac ggacggtcag ctgcgcctct tccccggcgc
    13081 gaaggtcgag atcaagacca agggatgagc atcaccgagc ccttcatccg aaggcccgtc
    13141 atgacgacgc tcgtcatggc ggggatcctg ctgttcggga cgatcggctt ccgctcgctc
    13201 ccggtgagcg acctcccgaa cgtggacttc ccgacgatcc aggtcacggc cggcctcccg
    13261 ggcgcgagcc cggagacgat ggcggcctcc gtcgcgacgc cgctcgagcg ccagttctcg
    13321 gcgatcgccg gcctcgactc gatcaactcg acgagctcgc aggggacgac gcaggtcacg
    13381 ctccagttcg cgctcgaccg caacatcgac gccgcctcgc aggacgtcca gacggccatc
    13441 gcggcggccg cgcggcagct cccgacgggg atgccgtcgc cgccgacgct caagaaggtc
    13501 aacccggccg accagccggt cctctacctc gtcctctcct cgccgacgat gccgctcacg
    13561 gcggtgaacg aggtcgccga gacgctcatc gcgcagcggc tttccacgat ctcgggcgtg
    13621 gcgcaggtcg tcgtcacggg cgcgcagaag cccgccgtgc gcgtgaagct cgacccgtcc
    13681 cagctcgcgg cccgcgggat cgggatcgac gaggtcgcga cgtccatcca gcggcagaac
    13741 tccaacctgc ccgtggggac gctctacggc gtccaccagt cgctcacggt gaaggcggac
    13801 ggccagctct tcgacgccgc ggcgtaccgg acgatgatcg tcgcctaccg caacggctcg
    13861 cccgtgacgc tcggcgacct cggcaaggtc gtggacgggg tcgagaacga ccggatcggc
    13921 agctggtacg acgacagaca ggtcgtgacc ctggcgatcc agcgacagcc gggctccaac
    13981 acgatcgccg tcgtggacgc ggtcaaggcg aggctgccgg ccctccgggc gcagctcccg
    14041 gccgcgctga agctcgacgt cctcttcgac cgctccgtgc cgatccgcga gtcggtgcac
    14101 gacgtggagt tcacgctcgt cctcacgatc gccctcgtcg tcctcgtcat cttcctcttt
    14161 ctgcggaacg tcagggcgac gctcatcccg agcctcgcgg tgccggcctc gatcgtcggg
    14221 acgttcgcgg cgatgtcgct cctcaacttc tcgctcgaca acctctcgct catggcgctc
    14281 acgctctccg tcggcttcgt cgtcgacgac gcgatcgtca tgctcgagaa catcgtccgc
    14341 cggatggaag gcggggagga cccgctgacg gccgcgctca agggcggcag ggagatcggc
    14401 ttcacgatcc tctcgatgac gatctcgctc gtcgccgtct tcatcccggt cctcttcatg
    14461 ggcgggctcc tcgggcggct cttcaaggag ttcgcgatca cgatcagcgt ggcgatcctc
    14521 gtctcggggt tcgtgtccct gacgctgacg ccgatgctct gcagccgctt tctgaaggcg
    14581 tcgcacgtcg cgaacgaggg acgtttcttc aaggcgacgg agcgcatctt cgacgcctgg
    14641 ctcgccctct acgagcggac tctcgtgtgg gtcctcgcgc gccagaagga gacgctgatc
    14701 gtctccggga tcctcctcct cgtcaccggg atcctcttcg cggtgatccc gaaggggttc
    14761 ctcacgagcg acgacacggg ccagttcatc ggctacacgg agggcctgcc gggcgtgtcg
    14821 ttcgcgcaga tgggccggca ccagaaagaa gtcacggaca tcatccgcag ccatcccgac
    14881 gtccggggcg tgtcgtcgac cgtcggggcg agcgacgcga gccctgcggg caactcgggc
    14941 agcctcctcg tcctcctcaa gccgcgctcg gagcgcaagc gggacgtcga cggggtcatc
    15001 gagcagctcc gccccaagct cgcggcggtg tccgggatcc gcgtcttcct ccagaaccct
    15061 ccgtccgtct cgatcggcgg ccaggtcacg cgcagcccgt tccagctcac gctccagggc
    15121 tcggatcgcg cccagctcgc gaagtacacg gagctgctgg agacgaaggt caagacgctg
    15181 ccgggcctca tcgacgtgac gaccgacctc cagaaccgga acccgcagct cgcggtgacg
    15241 atcgaccgcg acaaggcgtc cgcgctgggc ctcacggcgt cgcaggtcga ggacgcgctc
    15301 aacaacgcgt acgggacgcg ccaggtctcg acgatctaca cgtccacgaa ccagtaccag
    15361 gtcatcctcg agctcctcga gcagtaccag acgaaccccg cggccctgtc gcttctctac
    15421 gtgcgcaaca ccgcgaacac gctggtcccg ctctcctcgg tcgcgcggtt cgacgagagc
    15481 gccggccctc tcgcggtgaa ccacctcggg caggtcccgt cggccacgat ctcgttcaac
    15541 gtgaagcccg gcgtcgcgct gggcgacgcg acgtcgcgcg tgcgcaaggc ggcggacgag
    15601 atcctgccgc tcacgatccc ggcgacgttc cagggcacgg cggaggtgtt ccagtcgtcc
    15661 gtcgggggcc tcgcgtttct tctcgcgatc gccatcctcg tgatctacct cgtcctcgga
    15721 atcctgtacg agagcttcat tcacccgctc acgatcctgt cggggctgcc gtcggcgggc
    15781 ctcggcgcgc tcgtcacgct gctcatcttc gggaaggacc tcaacgtcta cgggttcgtc
    15841 gggctcatca tgctcgtcgg catcgtcgag aagaacgcga tcatgatgat cgacttcgcg
    15901 atcgagtcgg cccgcaccga aggaaagggc ccggccgagt cgatccggag ggcggcggtg
    15961 gtccgcttcc ggccgatcat gatgacgacg atggcggccc tcatgggcac ccttccgatc
    16021 gccctcggaa tcggcgccgg cgccgagtcg cgtcagccgc tcggcctcgc ggtcgtcggc
    16081 ggcctcctcg tctcccaggt gctgacgctc tacctcacgc ccgtcgtcta catcgcgttc
    16141 gattcgctcc aggcgaagct cacgcccgag gcgcttccgg acctgccgga ggacgcagag
    16201 ccggagagag ccgcgtgagc gagaagagtc tgaagccggt ccaggcgaac gtctccgcgc
    16261 tcttcatccg gaggcccgtc atgacgacgc tcgtcatggc ggggctgttc ctgttcggtg
    16321 cgatcggcta cgtctcgctg ccggtcgcag acctgccgaa ggtggacttc ccgacgatca
    16381 acgtgggcgc aagcctcccg ggggcgagcc ccgagacgat ggccgccgcg gtcgcgacgc
    16441 cgctcgagcg gcagttctcg gggatcgcag ggatcgactc gatgaactcc acgtcgagcc
    16501 agggctcgac gaacatcacg ctccagttct ccctcgaccg cgacatcgac gccgcggcgc
    16561 aggacgtcca gacggcgatc gcggcgacgc tctcgcagct gccgccgtcc atgccgaacc
    16621 cgccgagcct ccgcaaggtc aatccggcga tgcagccgat cctcttcatc gtgctgtcct
    16681 cgccgaccct cccgatgtcc gaggtctccg agtgggccga gaccgtcctc gcccagcgca
    16741 tctcgaccgt gacgggggtc gcgcaggtga acgtgtacgg ctcgaagaag tacgccgtgc
    16801 gcgtgcaggt ggatcccggc gtgctcgcga gccggcagat cggcatcgac caggtggccg
    16861 cggcgatctc gtcgggcaac tcgaaccgcc cggtcggggt cgtgtacggt cccgagaggg
    16921 cgctcgccat ccagacgaac ggccgtctcg tgaacgcgga cgcgtacaag ccgctcgtcg
    16981 tcgcctggcg caacggcgcg cctgtgcggc tcggcgagat cgcgaacgtg ctggacagcg
    17041 tcgagaacga gcggcaggcg gcgtggttca acggcaaccg gtcgatcatc ctcgcggtcc
    17101 agcgccagcc ggactcgaac acgatccagg tcgtggacgc cgtgagggcg ctcctcccgg
    17161 cgttccggat gcagatcccg gcggcgatgc gcatggacgt catgttcgac cggtcgcagg
    17221 gcatccggga aagcgtgcgc gacgtccaac tcacgctcgt cctcacgatc gtcctcgtcg
    17281 tcttcgtgat cttcctgttc cttcggaacc tgcgctcgac gttcatcccg tccattgcgc
    17341 tcccgacgtc cctcttcggc acgttcgcgg tcatggccgt gctcggcttc agcctcgaca
    17401 acctctccct catgggcctg acgctctcgg tcgggttcgt cgtggacgac gcgatcgtcc
    17461 agctcgagaa catcgtccgc cggatggaga agggcgagag cgccgcggtg gcgtccctgc
    17521 gcggctccag ggaaatcggc ttcacgatcc tgtcgatgac ggtctcgctc gtcgcggtct
    17581 tcatccccgt tctcttcatg ggaggtctgc tcggccggct cttccaggaa ttcgccgtca
    17641 cgatcagcgc cgcgatcctc gtgtcgggct tcgtgtcgct gaccctgacg ccgatgctct
    17701 gcagccgcct cctgaagcgc gaggagcccg gggccgcacg ccaggggcgg gtcgcggccg
    17761 ccctcgagag cggcttcaac cgcatcctga aggggtacga gcggacgctc cacgtcgtcc
    17821 tcgagcaccg gcggatcacg ctcgcgacgc tcgtcctgat ggccgtcctc gcgggcttcc
    17881 tgttcaagct cgcgccgaag gggttcatcc cgaacgagga catcgggatg ctgcgggcga
    17941 gcgtcgaggg tcagcagggg atctcgtatc aggacatgcg cgaccgccag agggccgcgg
    18001 ccgaaatcgt gcgcgccgac ccgggcgtcc agtcggtcca gtcgtccgtg ggcgccggcg
    18061 gcccgaactc gaccgcgaac tcgggctcga tgttcgtgac gctgaagccc cgcggccagc
    18121 gcgactcctc cgacgcgatc gtcgaccgcc tccgcccgaa gctcgcgaag gtggcgggcc
    18181 tgcgcgtctt catgcagacg ccgccggcga tcagcatcgg gggtcaacac acgcgcagcc
    18241 tgtaccagct cacgctccag agcaccgaca ccgaggctct ctacgcgaac gcggcgctcc
    18301 tcgagcggaa gatgcgcggg atcccgatca tccgggacgt gacgagcgac ctgcagatca
    18361 agaacccgca gctcgacatc acgatcgacc gcaagaaggc ttccgccctc gggatcacgg
    18421 cggggcagat ccagcgggca ctggcgctcg cgtacgggtc gagccagatc tcgacgatct
    18481 acacggccac gaacgaatac gaggtcatcc tcgaggtgaa gccgcaggac cagcgcgacg
    18541 cctcgtccct ttcgtcgctc tacgttcggt cgggcaacgg cacgctcgtg cccctcacgg
    18601 cgatcgcgac ggcgaggacg acggtcgggc cgcttctcgt tgcgcatctc ggccagctcc
    18661 cgtcggcgac gatctccttc aacctcggga aggacgtcgc gctcagccag gcgacggccg
    18721 ccgtcgagaa gatggcgagg gagacgcttc ccggctcgat cacgacgagc ttccagggca
    18781 cggcgcaggt cttccagtcg tcgttgaagg gcatgtcgat cctgctcgtc gtggccgtcc
    18841 tcgtgatcta cctcgtcctc ggcgtcctct acgagagctt cgtgcacccg ctcacaatcc
    18901 tgtccgggct gccctcggcc gccgtcggcg cgatcttcac gctgatcatc ttcggcaagg
    18961 acctgaacgt ctacgcgttc ttcggcctcg tgatgctcat cggaatcgtg aagaagaacg
    19021 cgatcatgat gatcgacttc gcgctcgccg cgcagcgcgg cgaggggatg gagcccgaga
    19081 aggcgatcgt cacggcgtcg ctcacgcgct tccggcccat cctcatgacg acgatggcgg
    19141 cgctcgcggg cacgctcccg atcgccctcg gcctcgggcg cgggggagag tcccgccagc
    19201 cgctcggcct cgccgtcatc ggcgggctcg tggtgtcgca ggtcctgacg ctctacatca
    19261 cgccggtcgt ctacctcgcg ttcgaatccc tcggcgcgcg cttccgcgcg cggaagacgg
    19321 cgacggcgca ggagccagca cccgcctgac ttctgctttc ggattcggat tcggaaatgc
    19381 ttcgaagggt gagtgagcga cgcgcgcccg tgagatgctc cggcgcggcc atctgctcgg
    19441 gaccactcta agaagtatct cctcggaggt gtccatgttg ctctggcgtg tcctcgctgt
    19501 atcgggtctg tgcctttctc tcactctgca tgcggtggag cccgcaccgt ccccggacga
    19561 cgacccgcgc gtggcgtcgg cactggagct cgcgcgcgcg tggctcgacg ccgagcgcgc
    19621 ctacgcgcag gtccccgggg tttcggcggc catcgtccac gaccagaagg tcctgtggag
    19681 cggggctttc ggcctggccg acgtggcgag cggccgcaag gccgagcccg gcacgatcta
    19741 cagcatctgc tcgatctcga agctcttcac gagcatcggc gtcatgcagc tgcgcgacgc
    19801 gggcaagctc cgcctcgacg atcccgtccg ctcgcgactg ccgtggttca cgatgaagcc
    19861 cggcggcacg gacgggaccg agatcacggt cgagggcctc ctcacgcacg cggccgggct
    19921 gccgcgcgag accgatctcc cgtactggac cggcccctcg ttcgcgttcc cgacgcacga
    19981 gcagatcgtc gagaggctcg cccagcagac ggcgctctac ccggcggcga cgtacatcca
    20041 gtactcgaac ctcggcctga cgctcgcggg ggagatcgtg gctgccttgt ccggcatttc
    20101 gtacgccgac tacgtcgcga ggaacatcct cgtccccctg aagctcgcgt cgacgacgcc
    20161 cgaaatgccc gagtccgagc gaggcaagcg cctcgcgagc gggtacagcg cgctgacccg
    20221 agcgggccgg cgcgatcccg tcgcgttctt ccagggcaag ggcgtcgcgc cggcgatggc
    20281 cttcgcctcg acggccgagg acctcgcgcg cttcgcgtcg tggcagttcc ggctgctcgg
    20341 cggcggaggc ccggaggtcc tgaagaccgc cacgctgcgc gagatgcacc gcgtccattt
    20401 cgtggatccc gacttcgaga cgatgtgggg gctcgggttc gaggtcagga ggagcgagaa
    20461 gacgacgctc gtcgggcacg gcggctcgtg ccccggctac cggacgcatc tcatgctgca
    20521 gccggacgaa aagctcgcgg tcgtcgtcct cacgaacgcg tcgggggtcg acgcggaggg
    20581 ctgggccggg ctcgtgcgcg agatcgtcgc gcccgcgctg aaggccgcgg caaaggaggc
    20641 cggcaagggc aagactcccg ctccggagat gaggaagttc gcggggacat actcgggtgc
    20701 accgtggggc ggcgagacgt tcgtcctccc gtgggaagac ggtctcgcga tgctgtccct
    20761 cccgtcgaag aacccgatga aggacctgat caagatgaag aagacggggg agaacacgtt
    20821 ccgccggatc cggaaggacg acacgctcgg cgaggagatc gtcttcgcgc tgggtcccga
    20881 cgggaaagcg acgcgcttca cccgccacag caactattcg acgcggatgg aatagcgggg
    20941 cgtcggctcc tcagtccgcg agcggccggt tgaattccgt gcggtactcc gccgggtcga
    21001 ggccggactc cggaccccgg aggtaacgct cccagagatc gccggcgggc gcgaggcctt
    21061 cggccttgat ccacgcgtcg aaccttcccc agccggaggc gaggccctcg aagccgccct
    21121 tgtggaccgt gcgggccatg cgcgtcgcgg gccacgtgcc gggtttcacg cggccggcgg
    21181 gggagatcgg cttcgagacc gggacgccga tctcgaagtc gaagacggcg ggatccatct
    21241 tcaggtggtg cgtgaaccac gggccggtga ccgccgcgcc ctgcgcgacg acggcggcca
    21301 tgacctcctg caggccggac cccatgacct tctggatctc gctgcgcgga atggtgacgc
    21361 gcaggagcgc gacgtcctgc gcgaccgtct gcacgacgtg cggctcgtcg agaatgttcg
    21421 agaggcggtc gtagctcacg gagacgccgc gctccattcc ggatttcaga acgccttcgc
    21481 gggcctcggc cgaggcatag agcagggtca tcgtcacctt cgtgcgtccg tccgtctcct
    21541 cgaacaccgt cgtgacgagg cactcgccgg gataccagga ctcgtcgaac cgttccgtgt
    21601 tgacgagccg ggagggccgc gcgatctcgc ggtagacgcc gcggattccc atcgtgatgc
    21661 ccttgtcctc gtttctccac tcgtagcggt acccgccgcc aaccttcagg tcgatcgcac
    21721 agacgggcat cgtccagccg tcggggccga ggagccaccg cctcacgagc tcgggcttcg
    21781 tgaaggcgtc gaagacgagc gtgcgcgggg cgtcgaacgt ccgggtcatg acgatctcgc
    21841 gctctccgcg cgccgcgacg tcgagggtac cggcgaaggg gacgggcatg ggacctcctc
    21901 gagagtcagc gaggtctgcg tctgcgtttc ggcttctgaa cggccttcat ctcgtcgaga
    21961 agggcgtcga gacgctggaa gcggcgttcc cagaacttcc ggtagttctc gagccactcc
    22021 accgcttctt cgaggggctt cgcctcgagg cggctcgggc gccgctgggc gtcgcggccg
    22081 cgggtgatga gcccggcgcg ctcgaggacg cggaggtgct tcgagacggc cggctgcgtc
    22141 atgtcgaacg gctcggccag ctcggagacc gaggcttcgc cggacgcgag gcgcgcgagg
    22201 atcgcgcgcc gcgtggggtc cgcgagcgcg aagaacgtgg cgtccaggcc ggccggagac
    22261 ttactataac cgacaggttc cataaccgat cggtaagata aagctgaaac gccggcctgt
    22321 caagggtcga atcggactac tccgagccgt ccgcccgcga gacgctcacg gtgcgctctt
    22381 cgtcctcgcc ctcgacccgc aggagccacg ggttgcagca gacctcgcag tcctggacga
    22441 ggctgccgcg gacgtcggac tcgagaatca cctcgacgtc ttctccgcag taggggcagg
    22501 tgaccacgaa atcctcgctc attcgcgcga gcggggaggt cccgtttcct cgttcaggcg
    22561 atcgcgcatc ttgcgggcct ggtgctcgaa ctcggggagc tgcgggtcgt ccgaggcgcg
    22621 ggcgagctcg agcgctcggt ccgcggccgc ggcggcgtcc ttccagcggc cgaccttcgc
    22681 gtaaccgtcc ttgagcccgt cccaggcgtt ggccgagtac gggtgtgcgt cgacgtttcg
    22741 ccggaagagc gcgatcgcct cctccgtccg gccgcgctcg agctccgcgt aggcgacctc
    22801 gttgacggcg ctctccgtgg gcgcaaccgg ccagccgagc gtcttcgaga acgcctcgta
    22861 gtgcctgttc accgcggcga tcccgccgtc cgcgagtccc tcgggcatcc ggtagccgtc
    22921 gtagagggcg cgcagcgcgt ggatgctgcc gacgagcggg atggaccggt ggcgctcctc
    22981 cggaaacgcc tcggtcttcc agcggaagcc cgggaccttg cgcgcgccga gggccgcgac
    23041 gagctcgtcc cagtccgcga gcgcctggcc cgagtcgtcc gaccgcgcgg cgtagaggaa
    23101 cgcgctcagg gacgacgcgg actcgagagc cttccggagc gagcgcgccg ggagccgatg
    23161 gtcccagtcg aggctcgggc tcagtgcgac gtaggcgcgg aagagcgcgg gctcggacgt
    23221 cagcgtgtag aggacgaact ggccccctgc cgagtgcccg aggagcgtcc gaaagccgtc
    23281 cgtccggtac gtccgttcca cgagaggaat gagctcggtc gagaggaagc gcttgaagtt
    23341 cgccgcgccg ccgccgccga cccaggcctc cttccagtcc gtctgcgtga agtctcggat
    23401 gcgcacggtg ctcgcgaccg cgacgacgat cgtctcgggc atctcgccct ctgcggcgag
    23461 gaagtccgcg gacgcagcca tgtggcggaa gtgcgtcctg ccgtcgagga cgtacaggac
    23521 cgggtagcgc cggtccttcg cccacggata gctggcagga agatggacct ggtacgtccg
    23581 atcctcgttc aacaccttcg agcggatgcg gaacgtttcg ccgatcgtga cgggctcgcc
    23641 ggcgaaggcg tgcagcggga ggacggtcag gagcgcgagc gcggacgcgc gaagaatcgc
    23701 caccgccgga cgataccaag tctcaatctg accggcgcac gcgccgaccg aggagcgccc
    23761 gcgctacatc ggcgagacgc ggccgatctc gatcacgtcg ggccggtccg tcgtcgccgt
    23821 gacgactccc gacgcgtcgc gcgagacgcg gacgacaggg gagatctcgt ccgccttcga
    23881 cgcgggaacc gagacgacgt tgtattcgac ctgcgcgacg gccttgaagt gcggctgctc
    23941 ggcgatcggg acgaaaacga tgaacatggt cccctgcacg cgggtgttcc atctcatcat
    24001 ttcgacggtg cccccgggtg ccacttcgac ggggccgagg gtttcacctg tcgcccatct
    24061 ggtgagcccg agcgcgccgg gcgaaacgtt ctggccgacg acgtggacca cgccgctgtt
    24121 cgacgcggga agcgacgggc ccgtcggcgt ttcggacctg aagcaggcgg acgcggcgag
    24181 ggcgaggacg gcggcgagcg tcggccccgc gagggttcga acgttcatgg gctggctcct
    24241 tgtgtgaggt gccacgaagg cggttgaagg tgaaccgcct cgccgttgca acggggatgc
    24301 cgcgcggatg actcgcgctc cgagccgttg ggattctcta cggctcgccg accgaatggc
    24361 taccgatgga acccgactcc gagcgcgatc tcgacgacga gaaccgcggc gacgagaaag
    24421 tccacggcgg cgcagaacgg cggccaccat cgtgttccgg aaacgaactg gctgcgctcc
    24481 tgccaccagt ccttgaagcc atggccggcg tagccgagga cgagaagcca cggcgatccg
    24541 gtgacggcgg cggcggcgag caggaggaag gcgcccgtga cgcagcattc gacggcgatg
    24601 accctgggcc gcccgtctgc caccgcgaac ccgatgtaga ccgccgcgat cagggccagc
    24661 aggagcgact ggacggtcgc ctggtcgagc caccggaggg cgagcggcga ggcggcctgg
    24721 atggcgccga cggccagtcc ccacaggaca ggggagcggc ggctcgtcag agttctccga
    24781 ccgccgacgc gagacggttg aggctcgtct tggcgtcgcc gaagaggagg cccgtgaccg
    24841 gcttgaagaa cagcgggttc tcgaggcccg agaagccctt cccgcgcccg cgcttcagga
    24901 cgatgacgga cttggccttg tcgacctcga tgatgggcat cccggcgatc gcgctcttcg
    24961 ggttgtcgcg cgcctcgggg ttcacgacgt cgttcgcccc gatgacgacc gcgacgtccg
    25021 tcgtgggcat ctccgggttg atctgctcga gctcgtagag ctgcgagtac gggacgttcg
    25081 cctccgcgag aaggacgttc atgtgccccg gcatgcggcc cgccacgggg tggatcgcgt
    25141 acttcacctg cacgccgcgg ttctcgagga ggctcgcgag ctcgcgcacg gcgttctgcg
    25201 cctgcgccgt cgccatgccg tagcccggca cgaagacgac ctgccgggcg taggcgagct
    25261 gcgtcgcgac gtcctccagc gagacctcgc gcatcacgcc ttcctcgccc cccgccgccg
    25321 cctcggtcgc gctgccgaac gcgccgaaga ggacgttcgt gatcgagcgg ttcatcgcct
    25381 tgcacatgag gatcgagagg atgaagcccg acccgccgtc cagcgtgccc gcgatgatga
    25441 ggacgttgtt cccgagcgca aagcccgtgg ccgcggacgc gaggcccgcg tacgagttca
    25501 ggagcgcgat cacgacgggc atgtccgcgg ccccgatcgg gagcacgagc agaacgccga
    25561 agaggagcgc gagcgcgcac atcgcgtaga agagagccgt gtgccccgga ttcaggacga
    25621 gcgcgatgaa gatgacgacc gcgctcccga agaggaggaa gttcaggacg ttctggccct
    25681 tgtacgtgat cggctgcccc ttcacgagct cctggagctt cgcgaacgcg acgaggctgc
    25741 ccgtgaacgt gatgccgccg agcaggacct cgaagccgag cgccgtgacc ttgaacgtgc
    25801 cgaggtgctc gccgtgccgg aggaactccg agacgccgac gagggccgcc gcgagtccgc
    25861 cgaacgcatg cgagagcgcc gtccgctgcg gcatcgccgt catcgggacc cacaggccca
    25921 tcgcgatgcc gatcgtcgag cccgcgagca ggccgacgat gatccactcg tagctgacga
    25981 tgtcgcggtg gagcagcgtg ccgatgaccg cgaggagcat gccgaactcg gccatgaaca
    26041 tgccgcgccg ggcggtgtcg ggatggctca gcccgcgcag gccgaggatg aagaggatcg
    26101 aggctgcgaa gtaggagagc tctatgagga gggtctgcgc gctcacttct tctccgccgc
    26161 cggtttcgct tcgcgcttga acatcttcag catgcggtcc gtgatgatga atccgccgac
    26221 gacgttgatc gtcgcggccg tcacggcgat gacgccgagg atcgtgctcg tgaggttgta
    26281 cttcgcgccc gccgtcacga gcgacccgat cagggagatg cccgagatcg cgttcgtcgc
    26341 cgacatgagc ggcgtgtgga ggagcggcgg gacgcgggag atgacctggt agccgaggaa
    26401 ggccgccagg acgaagacgt agaggccggc gatcaggact tcgttcatcg gggcgctccg
    26461 gtgggcgcgg ccggcgcgag gagcgcggcg acgcggggat tcacgggctt gccgtcgtgc
    26521 gcggcgcagg cgccggccac gacgtcgtcg ttcaggttca cggtgagggc gccgccctcg
    26581 cccacgagga gcttcaggaa ggccgccacg ttgcgggcgt agagctggct ggcatggagc
    26641 ggcacgctgg ccgcgagatt cagcggggcg aggatcgtga cgccgttcac gacgacttcc
    26701 tcgccgggct tcgtcagggc ggtgttgccg ccggtcggcg ccgcgaggtc gacgatgaac
    26761 gcgccgggct tcatgccgcg gacggcgtcc tcctcgagca tgaggggcgc cttgcgtccg
    26821 gggacctggg ccgtcgtgat cacgaggtcg gcgtccttca cgtgcctggc cacgaggtcc
    26881 cgcccgcgct tgagagcctc gggggaaagc tcgacggcgt agccgccggc gtcctgcgtc
    26941 tggatgccgc cgaggtcgac gtcgaggaat cgggcgccga gggagcggac ctcctcgccg
    27001 gcggcctgac gcacgtcgta ggcctcgacg ttcgcgccga ggcggcgcgc ggtcgcgatc
    27061 gccatgagtc ccgcgacgcc cgcgcccacc acgacgacct tcgccggcgg gaccgtgccg
    27121 gcggccgtca tgaggagcgg gaacatcttc gggaggcgcg cggcggccac gaggaccgcc
    27181 ttgtagccgg cgatcgtggc catcgaggac agcgcgtcca tcgcctgggc gcgcgtcgtt
    27241 cgcggcacga gctccatcgc gaaggcggtc acgccggcgt cgaggaacgg acggagcccc
    27301 tcgggctcgt ccagaggccg gaggaagccg atgacggccg cgccgcgccg gaggcgggcg
    27361 acgtccgccg gttccggacg gttgacgcag gcgaggaggt cggcgcccgc gaggagggcc
    27421 gcacggtcgg cctcgatcga ggcgccggcg gcggtgaagt cggcgtccgg gaggccggcc
    27481 tcgaggcccg ctcccgactg gacgacgacg gagagtccgg aggcgacgag cttcttgacg
    27541 gtgtcggggg ccagggcgac gcggcgctcg tcggcgcgcg cctccttcgg gatggaaacc
    27601 ttcatgcggg gccggatgat acagaagctc cgtctcggcg cctcgccgga cgccttgacg
    27661 ggcgaggccg ggcgacccac cttgaaggca gagcgcttcg ggagggccga tgtccatcct
    27721 cgaggcggcc gaggtccggt ctttcgtcac ctcggccgcg catcctcttc ccggcgggcc
    27781 cgccgaatac gaccctctcc tccgcgcgat cggggacgcc cgattcgtcc tcctcggcga
    27841 ggagtcccac ggcacgcacg agttcgcgcg cgcacgcgtg gacgtcacga tgcggctcat
    27901 ccgggagaag ggcttccggg gcgtggcggt cgaggccgac gggccggacg ccgcgcgcgt
    27961 cgaccggtgg gtccggggcg gcggggacga agccgacgga gccgatgcgc tcgcgggctt
    28021 ccggcgcttc ccgtcgtggc cgtggcgcaa cgccgacgtc ctcgacttcg tggagtggct
    28081 gcgcgcgcac aacgaagcga tcccggccgg gcgcccgaag gccggcttct ggggcctcga
    28141 cctctacagc ctccacgcgt cgatggagga ggct
//
LOCUS       DAOMFY010000019        27947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_554_length_27947_cov_7.893159, whole genome shotgun sequence.
ACCESSION   DAOMFY010000019 DAOMFY010000000
VERSION     DAOMFY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27947
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_27947_cov_7.893159"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="PLB01_03185"
     CDS             complement(<1..1733)
                     /locus_tag="PLB01_03185"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQQ76333.1"
                     /translation="MTRALRRPLAASFALLVGAAAWRAGARRPGPEDGTRLVLEHEEG
                     RAKHALSRAVAEAALARGVSGPPPASVEGSTRGFDVVAYDISFELDPGVRILAGSSTT
                     RLAGFAKSTVSVTFNLDRAYAVSSVTRDGAPVSGFTFANDLLTVPLVPPLAAEERTSI
                     GLSWSGIPPAAAALAWWDHATGRAATSVAEPFGSRQFWPCVDAPDDKAVATVTATVPP
                     GYVVASAGLGTSAVLPDGRIRSTWRLPQPISTYLVSLNVANYEVVEDTYTALDGRKMP
                     IRSYLMPENAAANVPRLSAMKDHISVLASLFGEYPFVDTKYGIVSSWFSGGMEHPTLT
                     SIGENILSNTPRDITLLLVHELAHQWWGDRVTMRTWDDIWLNEGFATYAEVLYQERAL
                     GKNPGQTLRTSYDDGLYGGRLSQAVVADPADPFKYTGAVYNKGGWFLHMLRRLVGDDA
                     FFSGLRAYGDAHAWGTATRGELRALFEERTGLDLKQFFDQWLETRFRPTFRATYRTAV
                     DASTVTLTVIQTQQHAVAHPVAGPNDKPYYVFPLTVRITYVDGTILDVPVTATGPTTT
                     LVVPNPSRKFVA"
     gene            complement(1762..3183)
                     /gene="hemG"
                     /locus_tag="PLB01_03190"
     CDS             complement(1762..3183)
                     /gene="hemG"
                     /locus_tag="PLB01_03190"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HQQ76334.1"
                     /translation="MKRVVILGGGIAGLTAAYRRKSEGETVVLEAGPAPGGSVKTVRE
                     DGFIVEGGPNTLRTSEAADRLLADLGLEPRVVKADAKAPRWIVRDGRPRAVVPGPGAL
                     FNTVFTTRGKLRLLKEPFVPGRPADLEDESVASFFARRFGPEAARYGAGPMVSGVYAG
                     DPDTLSTRSAFPRLWEAEGAAGSVIRGFLRKKKTEDPAQRGAGEAGANGAEDLLRKGK
                     KGAAAPARHRARTLTFDTGLYLLIETLQQRLVSRGACRIEVNSPAAIVEGPHPSGPKW
                     TVRLADGRSWDADVLVSTLEGPALARLLGDRLPRSGAGLAAMKTSPITVVVFAWPDGP
                     GSPKGFGALVPRGEGIRSLGVLYPSSLFSGRAPAGSILTTSFLGGALDPALAAATDAE
                     VLAVAAEEAKRLHPGLTAPERSWIFRWPAAIPQIPLFHHRTLAAVEKDLEFLPGLVLT
                     GGWRDGIAMGERIARGDALGAAL"
     gene            complement(3180..4160)
                     /gene="hemH"
                     /locus_tag="PLB01_03195"
     CDS             complement(3180..4160)
                     /gene="hemH"
                     /locus_tag="PLB01_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HQQ76335.1"
                     /translation="MSDSLGVLLVQLGGPASREELRPFLYELFVDPEILGISFSPLRR
                     FVAWLIATTRAPKSAATYERINWSPIRRWSETQARLLEAELGKGAAAPAPVVRVGMTC
                     SYPFVENALEDLRKAGATRLLVLPLYPQYSVTTTRGSFNRVTAALAKMGWNPARTNAP
                     DAWYDEPEFVAAHADLVRRAHATLPDPDPAKTVILYSAHSLPVKTVVTQKDPYPAHVE
                     GTVKAIDAALGNRFPSRLGYQSKVGPVEWLGPSTSDVLASLAKEGVTQVVVVPVAFVS
                     DHVETLYEIRLLFAGEAARLGIPHYAVAEGLNDHPAFVRALANIVTKAAL"
     gene            complement(4201..5631)
                     /gene="hemN"
                     /locus_tag="PLB01_03200"
     CDS             complement(4201..5631)
                     /gene="hemN"
                     /locus_tag="PLB01_03200"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HQQ76336.1"
                     /translation="MTAIPIRLRGETLPMTTELLDKYNVQGPRYTSYPTAPEWNEGIG
                     VKDYEEACARANARKSPVSLYAHLPFCDEQCWFCGCFMKVVPKPDRKGDDRAEIEPYL
                     ADLHREIDLLAKRIDPSRSIEQVHWGGGTPTYLTPVQADRLAQHLLETYRPAKGAEVS
                     VEVDPRVTTARHLEVLKNRGFNRVSMGVQDFDPEVQELVNRVQPFDMTKALVDASRSL
                     GYGSVNLDMIYGLPGQTRAGFEASVEQIISLRPDRVAMYSYAHVPWLKRPQRVLAAHL
                     PEGTEKFAIFVSGIERFVAAGYVYIGMDHFALPHDEIVKAQQDRTLTRNFQGYTTHAG
                     CDLYGMGVSAIGSVDDWYLQNTKDYAEYHAAMEAGRLATVKGCRVSADDVLRREVITR
                     LLCHCVIDKRDVEKTFSLKSFDETFANATAKLPDLVNDGMVEVTADEIRVTTMGRIFI
                     RNVAMLFDAYLEKKTEDSPKIFSRTL"
     gene            complement(5702..6991)
                     /locus_tag="PLB01_03205"
     CDS             complement(5702..6991)
                     /locus_tag="PLB01_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76337.1"
                     /translation="MITFLTLYLGIAAGLHTVEVQVDPAAAFVEFQLDGRTLHSVTQP
                     PWKMVVDLGRPVPHELVAIARDREGTELGRAVQLVNVPRPNSEASFTLLPGTGGSGRV
                     ARLTFASAFSARPESIELTFDGQVLDPGNLARIRLPDFRPADVHVLRAVLEFKGGSRV
                     SSELLVGGVKREAPGGPETMRGEAQTELTAVPVVFKGSPPAEYEMDGWFLANGEPVHV
                     EAVEESPGEIVVVKDENAGPWIENLRTVLYSRMSYPCPLRKGQRLRFCWPGTLKPSDT
                     APGYDVFLRSQDFSAPWDVRLLLTRVAPPEYSGPPRLGDAVGVAALSAAAGNRPRAVV
                     LVVAGGDDESQTPVAWTRAYLRALRVPLFVWAKNKKMVDPAWGKPEVLTSSSDVPGAV
                     KKVTKAVEAQRIVWLEGRHLPQSISLSPKARKVTLVE"
     gene            complement(7020..8705)
                     /locus_tag="PLB01_03210"
     CDS             complement(7020..8705)
                     /locus_tag="PLB01_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ76338.1"
                     /translation="MQRSLTFTLSAVLAFSPAVPASAQSDAPQPVRSSVEAVAADVEV
                     LVLDSKGKPVEGLAKGDFKLFVNGKETPIDWLEAPAAPSSASASAASASAPAPAASEA
                     PTGAAAARRMHSTVFVISDLQTDLRSRNAGLDALKGYVDRMPAREEAAVYLLDNGVRR
                     LQAFTSDRSTLKKALDKPARTLPRAYIFPQQGSVEWEGQSRQMLRNFSTVLDTIANRP
                     EPKTVVVMAGPISPTGFVQPIGGPAGSAALGETVLAGSPLSGVDVLSTSGDSYTARSI
                     WNFLAEAKDAESQALLARATIVAVDPTGLFSPDGRAEISSTSSPAGRPPRDTAAALRP
                     ANSVTEGDAVDSFAYRNDTFALIAQSTGGARLGFSNKPVENLIAETNLLAQRYRLGFT
                     PPDGTSARRDIRVQVARPGVVVRAAAGQRSLTPETAAHARFAALLLSADAPKGDFPIA
                     LETKAAVKDRKDDAFPFDVVVPVSGVYAEARGDTKRAKLELLIAGVDEEGRASEPMVI
                     PFSVTVDKSAGAGAFFRKDSNFTLDRRWKGRLFVGVRDTSTNLLGAVALPIGI"
     gene            8837..10015
                     /locus_tag="PLB01_03215"
     CDS             8837..10015
                     /locus_tag="PLB01_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76339.1"
                     /translation="MQILTYPVGVVVGLLPVVVELGAPPRPASLLLDGRPACTFVSDT
                     SACVVDLGAAPKVHLLELVRKGPSGNVVERAVRWVNRPGAAEAEVQTRARCVEGGSRC
                     SVRVGWAHPERLNPKKISVALDGVRAATTRNRDVSLDVAGTRGAVLTVDLVFPDGRRA
                     THASVLGGRAHGDEETAVVANIEAGACGPDGPAAAAARRRADGETVRAAERGEAEIVF
                     VAEPGAYGALASLLGSAHAVPKPAERVALDDILRKAGFAVHVVVPDVRLARGELQPRE
                     PVLDALLETLDATPLKPVRLADAVASAALHVGVPGKRRLVVLVAGEGAEDSSALSATP
                     VRNYLSEIHVPIVVWRLRPSVRPDWPQGRTIASMDDLRAAILDAKERLDCEAIVWTEP
                     "
     gene            complement(10020..11072)
                     /gene="hemE"
                     /locus_tag="PLB01_03220"
     CDS             complement(10020..11072)
                     /gene="hemE"
                     /locus_tag="PLB01_03220"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HQQ76340.1"
                     /translation="MSASTPSGPGPSRQRFVDAAFSRPVDVPPVWLMRQAGRYLPEYR
                     EVRKGLAFLDLCANVEGAVEVSMQPFRRFGMDGVILFSDILIPLPPMGIEVRLDEGGP
                     KLPSPIRGASDVARLRTFDPLVGTKFVPEIMKALKTEVAGRAAVIGFCGAPWTLATYA
                     IEGGGTKSFVEVKKMMHGDPKTLVSLLEKLADSCGDYLAAQIEAGADVVQVFDTWAGE
                     LSRADYDRFARPATERLLARLPKRGTVPVINFASGSAHLIESIAALPGDVVSVDWKLP
                     LDQARRRADGKALQGNVDPGVLLGPKEGVTAAVLAARRAAGPLGHIVNLGHGILPPTP
                     PENVGAFVAAARAPLG"
     gene            11146..12003
                     /gene="ccsA"
                     /locus_tag="PLB01_03225"
     CDS             11146..12003
                     /gene="ccsA"
                     /locus_tag="PLB01_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQQ76341.1"
                     /translation="MLDSAAAAAWSAGLGWTALTLYALASALYVLNLYRPSGWAGRSA
                     TACAVVGAVLNFAALYARALAVGTVPYRDLLGSMKLFGFFLAVLSVVLELRHHDRALG
                     PVLMPAALFFMLLALLEAPGGPRPAPNPDLKGAIFALHVTLNMLAYAAFAVSAALSML
                     YIAAGRALKSHTLSGPATRLPTLSYLERANRTSLGLGIIALGAGLALGFLWAARVWTN
                     EHPHWGLDPKIFFALGTLFFYLFVLVRARRGAPGVSTARLSLAGFVLVLLSYTAVNMF
                     VSKLHVFRS"
     gene            12000..13271
                     /gene="hemA"
                     /locus_tag="PLB01_03230"
     CDS             12000..13271
                     /gene="hemA"
                     /locus_tag="PLB01_03230"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HQQ76342.1"
                     /translation="MNLVFVGWNHRGAPLDLRERLAFTPEKAREALEGLFAERILAEG
                     AIVSTCNRAEIYGVSEREDDLDALSGYFSRFHRVDDVLLRQTALTGRGDATVRHLFRV
                     AAGLDSMVLGEAQILGQIREAHRVATAAGSLRAVTNRLFMNALECGKRVRAETGLGTR
                     PTSVAGLALTLAGRIFESLSGRRALLVGAGETAELTARLLLDEGVSEIVVTNRSFERA
                     QALAASAGGRAVPWDQRAVAAAEVDLILSATNATEPVLTTAALKASLSNAKRRGPLLV
                     LDLAVPRDVEVEVGELPDVFRYDLDALNGMADANAKARLEEVPKAEAIVEECVQRFTD
                     WWGSLLQVDVVRGLREKVEKIRLAEIEKYAGKLSGLSEKDRATVERLTETLVGHILHE
                     PTVGLKEGDASERLEKAASVKALFRLGEPPR"
     gene            13277..14257
                     /gene="hemC"
                     /locus_tag="PLB01_03235"
     CDS             13277..14257
                     /gene="hemC"
                     /locus_tag="PLB01_03235"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009716030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HQQ76343.1"
                     /translation="MTTAPPLRMGTRASALARAQSGAFAEELTRRSGRPVEVVIVRTQ
                     GDQLSSEGKAPPGGDSAGVFVRALDDALRNREIDFAVHSLKDVPTADAEGLVLAAVPA
                     RADSRDAFVVATRHAGVKRVEDLPQGAKVATSSPRRVSQLLRVRPDLVTVAMAGNVDT
                     RLQRLEEGRADALILASAGLDRLGRGAVITRRLEPDEMLGAPAQGALGIGCRADDEET
                     RISLLKMEDPIARAAAVVERDLLHFLRGGCRAPVGASASVVGGRLVLRGRVLSLDGRE
                     CLEDEMSEPYTPAFPSSPSKKSSLSSSLGSLLAEKLLARGAARLIDAARV"
     gene            14323..15021
                     /locus_tag="PLB01_03240"
     CDS             14323..15021
                     /locus_tag="PLB01_03240"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQQ76344.1"
                     /translation="MTVLHTHTVEARPEGVAEALAFDPVGATLVVSSKETVRVLLPLF
                     TRPFALMVAVGEGTAELLRKVNTGGRPRGTSGVSSTYSGAREAQIVVPATPGAAGVIH
                     LLRESSAAFPSRVLWPHGSDAASEPFEEIRSLGFSLLAPVVYVKRALSSLELDAGVVA
                     DFAAGRFGSVAVGSTAGLDVLLAAVPSPPPVRWGVLGPETAKAVAARNLPAPVVPPRA
                     RLSDLLDLLRKEPS"
     gene            15018..15989
                     /gene="hemB"
                     /locus_tag="PLB01_03245"
     CDS             15018..15989
                     /gene="hemB"
                     /locus_tag="PLB01_03245"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQQ76345.1"
                     /translation="MSFPLHRYRRLRRSESLRTMVRETSLAPSDFIAPLFVVPGKGVR
                     QGVSSMPGVDRTSAELAAKDAKALADLGVLSVILFGIPEHKDAKGTSSADPEGPVCQA
                     VKAIKAESPKTVVMTDICLCEYTDHGHCGVIKGDTVDNDPTLAILAAEAVAHAKAGAD
                     VVAPSDMMDGRVGAIRAALDKEGFADVALLSYAAKYASGFYGPFREAAESTPSFGDRR
                     AYQMDPGNVREAVKEILSDVAEGADMVMVKPALSYMDVIRAAKEATNVPLACYNVSGE
                     YAMVKAAAANGWIDGQRVTLEILTSFKRAGADLILTYHAKEASQWIG"
     gene            16130..17476
                     /gene="hemL"
                     /locus_tag="PLB01_03250"
     CDS             16130..17476
                     /gene="hemL"
                     /locus_tag="PLB01_03250"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HQQ76346.1"
                     /translation="MSEKKAGSARPVRRRLRRTTQSEALFLRAKKRMPGGVSSPVRSF
                     ASVGGTPFFVKKGKGATLVDADGNRYVDWVMSYGPLMFGHAYGPVLSAVRKALPRGTS
                     YGAPTKAEIELAERVAKAVPSMEKVRFVSSGTEAAMSAVRLARAATGRDLVLKFSGCY
                     HGHADSFLVSAGSGLATLGIPGSPGVPASLAGMTVTARYNDLDSVKALFAAHPKRIAV
                     IAVEPVAANMGVVTPEAGFLEGLRAIADMEGALLLFDEVISGFRLAKGGAQEIYGIRP
                     DLTCLGKIIGGGLPVGAYGGRADLMAQVAPDGPVYQAGTLSGNPLAMAAGKAMLDALT
                     PAVYAKLDRTARDLEHGMNAAAKKAGVADRVVLNRIGSILTPFFTKGPVRDFDDAKKA
                     DLGKFRVWYHAMLRHGVFVAPAQFEAMFVSTAHGPKEVAKTVKAHEIALKEAFAAA"
     gene            17473..18756
                     /gene="kynU"
                     /locus_tag="PLB01_03255"
     CDS             17473..18756
                     /gene="kynU"
                     /locus_tag="PLB01_03255"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HQQ76347.1"
                     /translation="MTPPDVSESLRREALERDREDPLARFRAEFVVADEGLVYLDGNS
                     LGRLPKKTEALVAKAVREEWGGRLIRGWNEGWVDAPRRVGDKLARLLGARPGEVVVSD
                     STSVNLFKLAVAALKAREARSTIVTDDLNFPSDLYVLDGAARLLGGGRRVVAVPARDG
                     TSVDPADLAAAIGPDTALVSLSHAAYRSGFLHDMRAVTEIAHRAGALVLWDVSHSAGV
                     VPIDLEAWGVDLAVGCTYKHLNGGPGAPAFLYVRRELQTSLENPVPGWFGREAPFTFA
                     PDFAPAAGVERFLVGTPPILSLAAAEAGVDLALEAGVPAARAKSLAMGRFLEDLWRAM
                     LEPKGVSLRSPSEPARRGSHLSFGHPEALAIDRALIAEMNVIPDFRPPDVIRFGFAPL
                     YTTFEELFEGVARFRGVLDEERWRRFAGEAGPVVT"
     gene            complement(18769..20442)
                     /locus_tag="PLB01_03260"
     CDS             complement(18769..20442)
                     /locus_tag="PLB01_03260"
                     /inference="COORDINATES: protein motif:HMM:NF017369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76348.1"
                     /translation="MPRARFLLVAAALAAVVAAARPVSGDDAFTRTGVIRHLVEDDFA
                     ASKSSNVYELETEEGDVLPLRFSTPPDVRTGDRVSVTGWPGRRAFEVDEIHSLGPGRS
                     ALGRTALSAWTTGPKRVLVMLLNFTDDSSFNATTVTNTQTLFFGAGSSVARYYAEASF
                     GLTTMTGDVVLVTATVAKPTTCDTGTIQSQANARALAAGYNPANYNFPVWVFPSISAC
                     GWSGLGYVGGGGSWIKGAPTGSWGLLVAAHELGHNFGVVHAHSYTCPSAAIAPSGCTR
                     SEYGDRFDTMGNSRAGHFNAQFKDTFGWLPGGSVKNHGGGTATYTIGALEAPGQSTYA
                     VRIPTSRSDRTYWIEWRGGTGFDAGQPSGVANGGLIRFAPSSVGGADLLDMNFATAGS
                     FDDAQLDVGKSFTDPELSLTITALSKTASSLTVQVDYGTPPPATSFHVLQPCRVFDTR
                     KPNGTYGGPTLYAGQVRTFPIRGQCGIPATAKSIAGNLTVTGSTAAGSVRLVPGGAPP
                     TGTATVAFRAGQTRANNVTMPLGAAGDVVVDGLPTGTAHILLDVVGWFE"
     gene            20698..22800
                     /locus_tag="PLB01_03265"
     CDS             20698..22800
                     /locus_tag="PLB01_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HQQ76349.1"
                     /translation="MSMVSRTALTLLAGLIAVPFTPVNRTATNNAPEQQAAPAAYAAT
                     AKEAYLAPEALTYIRPGFNIKIQAVTNFAAGQKPTVELYFTDDLGGPLDRLGLVTPGT
                     ISVRFIPAVWDPATRRYTDYLGYNADPAKNANPTRDNGTTGTWQELEVGHYKYTFGNA
                     FALPATMDATKPHTFAAMGVRNTTDIVGKPYYAVPKYLDFVPSTNTAAATFNAMGVAR
                     CNQCHDPLALHGGNYRDIKTCVLCHNPNNMRGNINPTEPREQFDGQIFFHALHASKNE
                     LIGEITYPQDLRFCTTCHDPAAAGGASWYTYPSVAACGSCHDDVNFTTGANHVAGPAA
                     DGTCTTCHKPEGSTDWDAGIKNAHVVPIQSKQLKGYNAKILSVANAGPGKKISVTFQL
                     TNGDGSFVDPGVYKDSTKGSISIKLGGPTTDYANVGMAANGQPFSEAAQTATYNATNG
                     QAVYTFTNAIPAAAKGTYVVSIDTRRPYTLSPAPKNGPTSINEGAPNQPFYFAVTGTT
                     VTPRRTSVALAKCNVCHARLDNLFSHGGQRIETQHCVICHAPNATDLGRRPADGSQNP
                     GESIEFARMIHRIHSGEELTQDYTIYGFGGNAANFNEVTYPGDRRNCLACHVNAAAYS
                     IPLPNTNIPVVTERDYYSPRGSTAAACTGCHDSRDVAAHAYINTAYFPNAPTVPVDSC
                     GTCHATGADAGVDKVHAR"
     gene            22814..23719
                     /locus_tag="PLB01_03270"
     CDS             22814..23719
                     /locus_tag="PLB01_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HQQ76350.1"
                     /translation="MKHLSLGVAFAALAAFAAGSAAAQAPAKPAAKVEAGPSCADCHD
                     EAKIFATNPHSRGYAAAHKGATGNAVCETCHGDGKKHMEAGGDKSLIKTLHGGRSTDT
                     CQTCHKQSAERASFWNSVHASSEAVNCLSCHSVHKSGYKQKALLKSDPNQLCATCHQD
                     VKASFTAMPYSHRLKDGVMSCVSCHDPHGRPANHAVRQTRAGEPGCVSCHAEKKGPFV
                     YPHVTGIAGSCSSCHRPHGSPYPHQLTRATVYTQCLECHSTLSTGTLGSQPPSFHNIS
                     LPRYQNCTTCHTAVHGSQRDPQLLK"
     gene            23730..25670
                     /locus_tag="PLB01_03275"
     CDS             23730..25670
                     /locus_tag="PLB01_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76351.1"
                     /translation="MRILRSAFLTAALTLVLASGVARAQAIPFDIEAGYRFLSVTGNE
                     EMYRSQINDREGFLIRSLHVGTDEKYAGFPLTDRFRLDAADLGAGGAGFLRFDAGLSG
                     VYRLRFSYRNTQYFSAIQGFANPLGATVGQQTWDRTRNQFNVDLEVLPGKVVTPIVGY
                     TSNTLSGPGYTTYHFSQDEFQLSQNLRSTDQEFRVGAAFNAGVVSGEVIQGWRKYHET
                     EDLTLAPFGGSGNNTSPVLGKNVSATSLTRSSTTDVNVPVTSAFVRGFALPNLQISGF
                     YVRSNATGDDGTKEGASGSFASFAISRFFTGYSANNTTSPNSLTWRAGGRAEWHVMSG
                     LDVSAGYTERDQTWDNQALLNELYTGTSTFTGFSQSDFQSLLSTQTNIERKDKVLDVQ
                     ASMRFLGPVGVRLGFSRIDQDLTVVEDPAEIVVPGNQGGTFSRSINRYEGALTFTMAG
                     FNIMGEASFDDANAAITRVDYLKRNRERVRLTWKTFDWIQLGGAAMWLDQKNEDSGPL
                     NTYTASSANYSVDLTLKPVKWAWLRGAYGKLEADSSIPIRVPQNFTVVNSVNTEDGEL
                     MDFGAGFKFKEFAVDGFWSRFDNTGSYLFRMYRGGARADWTASAHAGLVVEWTVDRYL
                     DLMQSTQSYRGDRLGVYLRWRP"
     gene            25703..26029
                     /locus_tag="PLB01_03280"
     CDS             25703..26029
                     /locus_tag="PLB01_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HQQ76352.1"
                     /translation="MKRITLAVFALVAAVTLVAGAAFATMDLQKEFLAKYPDSKMKCG
                     SCHTKAMPKKGDAEVNKFGADMNKVKKDGKYDFAALDKMDSDGDGVSNADELKKGTNP
                     GDPASK"
     gene            26040..27275
                     /locus_tag="PLB01_03285"
     CDS             26040..27275
                     /locus_tag="PLB01_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ76353.1"
                     /translation="MRLGVVLTAFLLSATPLRAAAPTPKAPSNDDCLGCHEDPSAKRA
                     DGSSLFVDKRVFGESVHGAAGAACVDCHTDLAKTKDFPHPEKLAKPDCSGCHAQQVED
                     YGKSWHAAARAKSPTSKAAWCADCHGSHDIKPSKDPDSRTNHFNLPNTCMRCHGDPKI
                     FPRAGGTDGTEPVSFHDSIHGKALEKSGLKVAPNCATCHGYHEIRSPKHDAKSTVARA
                     HVPSTCGSCHSKILDEYSTSIHGEQLAKGNESVPVCSDCHSAHAISAAPEVRRLTILK
                     QCGGCHDSSLKSYRDTYHGQVSELGFAAVATCADCHTAHEIHPAGDVRSSVGPAKRLA
                     TCQKCHPGAGPKFAKYDPHANPHDRGRSGILWFTALFMKYLLIGVFAFFGVHTALWFP
                     RSVKARREAERRGGNGDAR"
     gene            27272..>27947
                     /locus_tag="PLB01_03290"
     CDS             27272..>27947
                     /locus_tag="PLB01_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HQQ76354.1"
                     /translation="MKDHGTAGPAADAYVRRFDELDRRLHIFLMTSFLGLALTGLPLL
                     FSERKWAKVLSHLFGGFKAAGGLHRCFAVVMVIVFFVHLSRMMKRLFLDKDTTILWGP
                     GSMVPQPRDLSEMLGHIRWFVGLGPRPNFDRFTYWEKFDYWAVFWGMGIIGGSGFILW
                     FPKFFSRALPGDWFNVALLVHGEEALLATVFIFTIHFFNGHLRPEKFPMDTVIFTGVL
                     PLEEVKH"
BASE COUNT         4220 a         9830 c         9704 g         4193 t
ORIGIN      
        1 gcgacgaact tcctcgacgg gttcgggacg acgagcgtcg tcgtcgggcc cgtcgcggtc
       61 acgggcacgt ccaggatcgt cccgtccacg tacgtgatcc gcacggtcag cgggaacacg
      121 tagtagggct tgtcgttcgg cccggcgacg gggtgggcga cggcgtgctg ctgcgtctgt
      181 atcaccgtca gcgtcaccgt cgacgcgtcg acggccgtcc ggtacgtggc ccggaacgtc
      241 ggccggaacc gcgtctcgag ccactggtcg aagaactgct tgaggtcgag accggtgcgc
      301 tcctcgaaga gcgcgcgcaa ctcgccgcgc gtcgccgtgc cccacgcgtg cgcgtcgccg
      361 taggcgcgca gcccggagaa gaacgcgtcg tcgcccacga ggcggcgcag catgtggagg
      421 aaccacccgc ccttgttgta cacggccccg gtgtacttga acgggtcggc cggatcggcg
      481 acgacggcct gcgaaagcct gccgccgtag agcccgtcgt cgtacgacgt gcgcagcgtc
      541 tgcccggggt tcttcccgag cgcgcgttcc tggtagagca cctcggcgta cgtggcaaaa
      601 ccctcgttca gccagatgtc gtcccacgtc cgcatcgtga cgcggtcgcc ccaccactgg
      661 tgcgccagct cgtgcacgag gaggagtgtg atgtcgcgcg gcgtgttcga gaggatgttc
      721 tcgccgatgg acgtgagcgt cgggtgctcc atcccgcccg agaaccacga ggagacgatc
      781 ccgtacttcg tgtccacgaa cgggtactcg ccgaagaggc tcgcgaggac cgagatgtgg
      841 tccttcatcg cggagagccg cggcacgttc gcggccgcgt tctcgggcat gaggtacgag
      901 cggatcggca tcttccgccc gtcgagcgcc gtgtacgtgt cctcgacgac ctcgtagttc
      961 gcgacgttca gggagacgag atacgtcgag atcggctgcg ggaggcgcca cgtggaccgg
     1021 atgcgcccgt cgggcagcac ggccgacgtc ccgaggcccg cggacgcgac gacgtagccc
     1081 ggcggaacgg tcgccgtcac ggtcgcgacc gccttgtcgt ccggcgcgtc cacgcacggc
     1141 cagaactgcc gcgacccgaa cggctcggcg acgctcgtgg ccgcccggcc cgtcgcgtga
     1201 tcccaccacg cgagcgcggc ggccgcggga ggaatgcccg accacgagag gccgatcgag
     1261 gtccgctcct cggcggcgag cggcgggacg agcggcacgg tgagcaggtc gttcgcgaac
     1321 gtgaacccgg agaccggcgc gccgtcgcgt gtgacggagg agacggcgta agcgcggtcg
     1381 aggttgaacg tcacggagac ggtcgatttc gcgaagcccg cgaggcgcgt cgtggacgag
     1441 cccgcgagga tccggactcc cgggtcgagc tcgaaggaaa tgtcgtaggc cacgacgtcg
     1501 aatccgcgcg tcgagccctc gacggacgcc gggggcggcc cggaaacccc gcgcgcgagc
     1561 gccgcctcgg ctacggcgcg cgagagcgcg tgcttggcgc gtccctcctc gtgctcgagg
     1621 acgaggcgcg tcccgtcctc gggtccgggg cgccgggcgc cggccctcca ggcggcggcg
     1681 ccgacgagaa gggcgaagga ggcggcgagc ggccgccgca gcgcgcgggt cacccctcga
     1741 ttctacggcg cgaagccgcc ctcagagggc ggcgccgagc gcgtcgccgc gcgcgatgcg
     1801 ttcgcccatc gcgatgccgt cacgccagcc gcccgtgagg acgaggcccg gcaggaactc
     1861 gaggtccttc tcgaccgctg cgagcgtgcg gtgatggaag agcgggatct gcgggatcgc
     1921 ggcgggccag cggaagatcc acgagcgctc gggcgccgtc aggcccgggt ggagccgttt
     1981 cgcctcctcc gccgcgaccg cgagcacctc ggcgtcggtg gcggccgcga gcgcagggtc
     2041 cagcgcaccg ccgaggaacg acgtcgtgag gatcgagccg gcgggagcgc gcccggagaa
     2101 gagcgacgac gggtagagga cgccaagcga ccggatgccc tcgccgcgcg ggacgagcgc
     2161 gccgaagcct ttcggcgagc cgggcccgtc gggccacgca aacacgacga ccgtgatcgg
     2221 cgacgtcttc atcgcggcga gccccgcgcc cgaacgcggc agccggtcgc cgagaaggcg
     2281 cgcgagcgcc gggccctcga gcgtcgagac gagaacgtcc gcatcccacg agcggccgtc
     2341 cgcaaggcgc acggtccact tcgggccgga gggatgcggg ccttcgacga tcgcggcggg
     2401 cgaattcacc tcaatgcggc acgcgcctcg cgaaacgaga cgctgctgaa gagtttctat
     2461 gagtaggtaa agacccgtgt cgaacgtgag ggtcctcgcg cgatggcggg ccggagcagc
     2521 cgcgcccttc ttaccctttc taagaagatc ttcagcgccg tttgcccctg cttcgcccgc
     2581 gccgcgctgc gccgggtctt cggtcttctt ctttctaaga aatcctctta tcacgctccc
     2641 cgcagccccc tccgcctccc agaggcgggg gaatgcgctt cgcgtcgaca gcgtgtccgg
     2701 gtcgcccgcg tacacgcccg agaccatcgg ccccgcgccg tagcgcgcgg cctcggggcc
     2761 gaagcgccgc gcgaagaacg acgccacgct ctcgtcctcg agatcggcgg gacgcccggg
     2821 gacgaacggc tccttgagga ggcggagctt gccgcgcgtc gtgaagaccg tgttgaagag
     2881 ggcgccgggc cccggcacga cggcgcgcgg ccggccgtcg cggacgatcc agcgcggcgc
     2941 cttcgcgtcc gccttcacga cgcggggctc gaggccgagg tcggcgagca ggcggtcggc
     3001 cgcctcgctc gtccggagcg tgttcggccc gccctccacg atgaatccgt cctcgcggac
     3061 cgtcttcacc gagcccccgg gcgcggggcc cgcttcgagg acgacggttt cgccctcgct
     3121 cttccgccgg tacgccgccg tcaggccggc gatgccgccg cccaggatca cgacacgttt
     3181 catagggccg ccttcgtcac gatgttcgcg agcgcgcgga cgaacgccgg gtggtcgttg
     3241 aggccctcgg cgaccgcgta gtgcgggatg ccgagccgcg cggcctcgcc cgcgaagagg
     3301 agccggatct cgtacagcgt ctcgacgtgg tcggagacga acgccacggg gacgacgacg
     3361 acctgcgtca cgccctcctt cgcgaggctc gcgaggacgt cggaagtgga cggcccgagc
     3421 cactcgaccg ggccgacctt cgactggtag ccgaggcgcg acgggaagcg gttcccgagg
     3481 gccgcgtcga tcgccttcac cgtgccttcg acgtgggcgg ggtacgggtc cttctgcgtg
     3541 acgacggtct tcacgggcag ggagtgggcc gagtagagga tcacggtctt cgccgggtcc
     3601 gggtcgggca gcgtcgcgtg cgccctgcgg accaggtcgg cgtgcgccgc gacgaactcg
     3661 ggctcgtcgt accaggcgtc gggcgcgttc gtccgcgccg gattccagcc catcttcgcg
     3721 agggcggccg tcacgcggtt gaacgaaccg cgcgtcgtcg tgacggagta ctgcgggtag
     3781 agggggagga cgaggaggcg cgtggcgccg gccttccgga ggtcctcgag tgcgttctcg
     3841 acgaacgggt acgagcacgt catcccgacg cggacgacgg gcgcgggcgc ggccgcgccc
     3901 ttcccgagct cggcctcgag gaggcgggcc tgcgtctcgg accagcggcg gatgggcgac
     3961 cagttgatcc gttcgtacgt cgcggcggac ttcggcgcgc gcgtcgtcgc gatgagccag
     4021 gcgacaaatc ggcgaagggg agagaaggaa atgccgagga tttcaggatc tacgaagagt
     4081 tcatacagaa acgggcggag ttcctcgcgg gaggccggcc ctcccagttg cacgagcagc
     4141 acacccagac tatcgctcat agaccttcta agaaaatcaa cgtcacccgg aacccacccc
     4201 tcacagggta cgcgagaaga tcttcggcga gtcttcggtc ttcttctcga ggtaggcgtc
     4261 gaacagcatc gccacgttcc ggatgaagat ccgtcccatc gtggtcaccc ggatttcgtc
     4321 ggcggtgact tcgaccatgc cgtcgttcac gaggtccggg agctttgccg tcgcgttcgc
     4381 gaacgtctcg tcgaacgact tgagcgagaa cgtcttctcg acgtcgcgct tgtcgatcac
     4441 gcagtggcag aggaggcgtg tgatgacctc gcggcgcagg acgtcgtcgg cggagacgcg
     4501 gcagcccttc accgtcgcga gccgcccggc ctccatcgcg gcgtgatact cggcgtagtc
     4561 cttcgtgttc tgcaggtacc agtcgtccac ggagccgatc gcggagacgc ccatcccgta
     4621 gaggtcgcag ccggcgtgcg tcgtgtagcc ctggaagttc cgcgtgagcg tccggtcctg
     4681 ctgcgccttc acgatctcgt cgtgcgggag cgcgaagtgg tccatcccga tgtagacgta
     4741 cccggcggcc acgaagcgtt cgatccccga gacgaagatc gcgaacttct ccgttccttc
     4801 ggggaggtgc gccgcgagga cgcgctgggg gcgcttgagc caggggacgt gcgcgtacga
     4861 gtacatcgcg acgcggtcgg ggcgcaggga gatgatctgc tcgacgctcg cttcgaagcc
     4921 cgcgcgcgtc tgaccgggga ggccgtagat catgtcgagg ttcacggagc cgtagccgag
     4981 tgaccgcgaa gcgtcgacga gcgccttcgt catatcgaac ggctggacgc ggttcacgag
     5041 ctcctgcacc tcggggtcga agtcctggac gcccatcgag acacggttga atccgcggtt
     5101 cttcaggacc tcgaggtgcc tggccgtcgt gacgcgcggg tcgacctcga cggagacctc
     5161 cgcgcccttc gcgggccggt acgtctcgag gaggtgctgc gcgaggcggt cggcctgcac
     5221 gggcgtgagg tacgtcggcg tcccgccgcc ccagtgcacc tgctcgatcg agcgggaggg
     5281 gtcgatgcgc ttggcgagga ggtcgatctc ccggtggagg tccgcgaggt agggctcgat
     5341 ctccgcgcgg tcgtcgccct tccggtcggg cttggggacg accttcatga agcagccgca
     5401 gaaccagcac tgctcgtcgc agaacgggag gtgggcgtag agcgagacgg gcgatttccg
     5461 cgcgttcgcg cgggcgcagg cctcctcgta gtccttaacg ccgatcccct cgttccactc
     5521 gggcgcggtg gggtaggagg tgtagcgggg gccctggacg ttgtacttgt cgaggagctc
     5581 cgtcgtcatc gggagcgtct cgccgcgaag gcgaatcggg atggccgtca cgcggcggag
     5641 gttagcaccg gcgcgactgg gtctcgttgg gtcaggtgca tcaggcgcgc cgggacggcg
     5701 ttcattccac gagggttacc ttccgcgcct tcggggagag agagatcgac tgcgggaggt
     5761 gccggccctc cagccagacg atgcgctggg cctcgacggc cttcgtgact ttcttcacgg
     5821 cgcccggcac gtcggagctg ctcgtgagga cctcgggctt gccccacgcg ggatccacca
     5881 tcttcttgtt cttcgcccag acgaagagcg ggacgcgcag cgcgcgcagg tacgcgcgcg
     5941 tccatgcgac cggcgtctgg ctctcgtcgt cgccgccggc cacgacgagg acgacggccc
     6001 tcggccggtt ccccgcggcg gccgagagcg cggccacgcc gacggcgtcg ccgaggcgcg
     6061 gcggcccgct gtactccggc ggggcgactc tggtcaggag caagcgcacg tcccacggcg
     6121 cggagaagtc ctgcgagcgc agaaagacgt cgtagcccgg ggccgtgtcc gagggcttga
     6181 gggtcccggg ccagcagaag cgcaggcgct ggccctttcg caaggggcac gggtacgaca
     6241 tccgggagta gagaacggtc cggaggttct cgatccacgg tcccgcgttc tcgtccttca
     6301 cgacgacgat ctccccgggg ctctcctcga cggcctcgac gtgcacgggc tccccgttcg
     6361 cgaggaacca gccgtccatc tcgtattccg ccggcgggct gcccttgaag acgacgggca
     6421 cggccgtcag ctcggtctgg gcctccccgc gcatcgtctc gggcccgccc ggcgcctcgc
     6481 gcttcacgcc tccgacgagg agctcgctcg agacccggct cccgcccttg aactcgagga
     6541 ccgccctcag gacgtggacg tcggccggcc ggaagtcggg gaggcggatg cgggcgaggt
     6601 tgccggggtc gaggacctgg ccgtcgaacg tcagctcgat gctctcgggc cgcgcggaga
     6661 aggcgctcgc gaacgtgagg cgcgcgacgc ggccgctgcc tccggttccg ggcaggagcg
     6721 tgaacgacgc ctcggagttc gggcgcggga cgttgacgag ctggaccgcg cgccccagct
     6781 cggttccctc gcggtcccgc gcgatcgcga cgagctcgtg cggaacggga cggccgaggt
     6841 cgacgaccat cttccacgga ggctgcgtga cggaatggag ggttcgcccg tcgagctgga
     6901 actccacgaa cgcggcggcc gggtccacct gcacttcgac ggtgtgaagg ccggctgcga
     6961 tcccgaggta gagcgtcagg aaggtgatca cgcggcggcg ccgctttcgt cgccctgcgt
     7021 cagatcccga tcggcagcgc cacggccccg aggaggttcg tggacgtgtc gcgcacgccg
     7081 acgaacaggc ggcccttcca gcgtctgtcg agggtgaagt tcgagtcctt gcggaagaag
     7141 gctccggccc cggcggactt gtccaccgtg acggagaagg ggatcaccat cggctcggag
     7201 gcgcggccct cctcgtcgac gcccgcgatc aggagttcga gcttcgcgcg cttcgtgtcg
     7261 ccgcgcgcct cggcgtagac gccggagacg gggacgacga cgtcgaacgg gaacgcgtcg
     7321 tccttgcggt ccttcacggc ggccttcgtc tcgagcgcga tggggaagtc gcccttgggc
     7381 gcgtcggcgg agaggaggag cgccgcaaac cgcgcgtgtg ccgccgtctc gggcgtgagg
     7441 gaccgctggc cggccgcggc gcggacgacg acgccgggcc gcgcgacctg gacacggatg
     7501 tcgcgccggg ccgacgtgcc gtccggcggc gtgaagccga ggcggtagcg ctgcgcgagg
     7561 aggttcgtct cggcgatcag gttctccacc ggcttgttcg aaaagccgag gcgcgctccg
     7621 ccggtcgact gcgcgatgag cgcgaacgtg tcgttccggt aggcgaacga gtcgaccgcg
     7681 tcgccctcgg tgacgctgtt ggcgggccgc agggcggcgg cggtgtcccg gggcggccgg
     7741 cccgcggggc tcgacgtgga gctgatctcg gccctgccgt ccggcgagaa gaggcccgtc
     7801 gggtccacgg ccacgatcgt cgcccgggcg agaagggcct ggctctcggc gtctttcgcc
     7861 tcggcgagga agttccagat gctgcgcgcg gtgtaggagt ccccgctcgt cgaaaggacg
     7921 tccacgcccg aaagagggct cccggcgagg acggtctccc cgagcgccgc cgagccggcc
     7981 gggccgccga tcggctggac gaaaccggtc ggggagatcg ggcccgccat gacgacgacc
     8041 gtcttgggct cggggcggtt cgcgatcgtg tcgaggacgg tcgagaaatt gcggagcatc
     8101 tggcgcgact ggccttccca ttcgacgctg ccctgctggg ggaagatgta cgcccgcggg
     8161 agcgtccgcg ccggcttgtc gagcgccttc ttcagcgtgg agcggtccga cgtgaaggcc
     8221 tgcaggcggc ggacgccgtt gtcgaggaga tacacggcgg cttcctccct ggccggcatc
     8281 cggtcgacgt agcccttcaa cgcgtcgagg ccggcgttgc gcgatcgcag gtcggtctga
     8341 aggtcggaga tcacgaagac cgtgctgtgc atgcggcgcg cggcggcggc gcctgtcggc
     8401 gcttcggaag ccgcgggcgc cggcgccgag gcggaagcgg ccgaggccga agccgacgac
     8461 ggtgcggccg gcgcctcgag ccagtcgatc ggcgtctcct tgccgttcac gaacagcttg
     8521 aagtcgccct tcgcgaggcc ctcgacgggc ttgcctttcg aatcgaggac gaggacttcg
     8581 acgtcggcgg cgaccgcttc gacggaactc ctgaccggct ggggcgcgtc ggactgcgcg
     8641 gaggcgggga cggccgggga aaacgcgaga acggccgaga gtgtgaaggt cagggaacgc
     8701 tgcatggagg cctccggaca gcctcttagc acgccccgtg ccggttgtca cagggtccgg
     8761 acgtctcccg gcggggacgg aaggcgtgaa aacgaggccg aacgcgcggg cgtttgtttg
     8821 ctacgttcgg gttcgcgtgc agatcctgac ctatcccgtt ggcgtcgtcg tcgggcttct
     8881 ccccgtggtc gtggagctcg gcgcgccccc gcggcccgcg tcgctgctgc tggacggccg
     8941 gcccgcgtgc acgttcgtga gcgacacgtc cgcgtgtgtc gtcgacctcg gcgcggcccc
     9001 gaaggtccat ctcctcgagc tcgttcgcaa gggcccctcg ggcaacgtcg tcgagcgcgc
     9061 ggtgcgctgg gtgaaccggc cgggcgccgc ggaagcggag gtccagacgc gcgcgcgctg
     9121 cgtcgagggg ggcagccgct gctccgtccg ggtgggctgg gcccacccgg aacgcctcaa
     9181 tccgaagaag atctccgtgg cgctcgacgg ggtgcgcgcc gccacgaccc ggaaccggga
     9241 cgtgagcctc gacgtcgcgg ggacgcgcgg cgcggtcctg accgtcgacc tcgtgtttcc
     9301 ggacgggcgc cgcgcgacgc acgcgtcggt cctcggaggg cgcgcgcacg gggacgagga
     9361 gacggccgtc gtggcgaaca tcgaggccgg cgcctgcgga cccgacggtc ccgcggcggc
     9421 cgccgcgcgc aggcgggcgg acggagagac cgtccgcgcg gccgaacgcg gggaggccga
     9481 gatcgtcttc gtggccgagc ctggcgccta cggcgccctc gcgagtctcc tcggatcggc
     9541 gcatgcggtc ccgaaaccgg cggagcgcgt cgccctcgac gacatcctga ggaaggccgg
     9601 gttcgcggtg cacgtcgtcg ttccggacgt tcgcctcgcg cgcggggagc tgcagccgag
     9661 ggagccggtt ctcgacgcgc tcctcgagac gctcgacgcg acgccgctca agcccgtgcg
     9721 gctcgcggac gccgtcgcgt cggcggcgct gcacgtcgga gtgccgggca agcgccggct
     9781 cgtcgtcctc gtcgcggggg agggcgccga ggactcgagc gctctctcgg cgacgcccgt
     9841 ccgcaactat ctctccgaga tccacgtccc aatcgtggtc tggcgcctgc gcccgtcggt
     9901 gcggccggac tggccgcagg gccggacgat cgcatccatg gacgacctcc gcgccgcgat
     9961 cctggacgcg aaggaacgcc tcgactgcga ggcgatcgtt tggaccgagc cctgaagccc
    10021 tagccgagcg gcgcgcgtgc ggcggcgacg aacgcgccga cgttctcggg cggcgtcggc
    10081 ggcaggatgc cgtggccgag gttcacgatg tggccgagcg gccccgcggc cctgcgcgcc
    10141 gcgaggacgg cggccgtcac gccctccttc ggcccgagga ggacgccggg atcgacgttc
    10201 ccctgcagcg ccttgccgtc tgcacggcgg cgagcctgat cgagcgggag cttccagtcc
    10261 acggagacga cgtcgcccgg cagcgcggcg atcgactcga tgaggtgcgc cgagccggac
    10321 gcgaagttga tgacgggcac cgtgccccgt ttcgggagcc tcgcgaggag gcgctccgtc
    10381 gcgggacgcg cgaagcggtc gtagtcggcg cgcgagagct cgcccgccca cgtgtcgaag
    10441 acctgcacga cgtccgcgcc cgcctcgatc tgcgccgcga ggtagtcgcc gcacgagtcc
    10501 gcgagcttct cgagtagcga gacgagcgtc ttcgggtcgc cgtgcatcat cttcttcact
    10561 tcgacgaacg acttcgtgcc gccaccctcg atcgcgtacg tcgcgagcgt ccagggcgcg
    10621 ccgcagaacc cgatcacggc cgcgcggccc gcgacctcgg tcttgagcgc cttcatgatc
    10681 tcggggacga acttcgtgcc gacgagcggg tcgaacgtcc tgaggcgcgc gacgtccgac
    10741 gcgccgcgga tcgggctcgg gagcttcggc ccgccctcgt cgaggcgcac ctcgatcccc
    10801 atgggaggga gcgggatcag gatgtccgag aacaggatga cgccgtccat cccgaagcgc
    10861 cggaagggct gcatcgagac ctcgacggcg ccctcgacgt tcgcgcagag gtcgaggaac
    10921 gcgaggccct tgcgcacctc gcggtactcc gggaggtagc ggccggcctg ccgcatgagc
    10981 cacacaggcg gcacgtccac ggggcgggaa aaggcggcgt cgacgaatcg ctggcgggac
    11041 ggcccggggc ctgagggggt ggaagccgac atggggcggg aggttagcag ttgatgcaga
    11101 cgcaggggtt gtgggcctcc ggggggttcc tataatcgcg ccgtgatgtt ggattcggcc
    11161 gcagcggccg cgtggagcgc cggcctcggc tggaccgctc tcacgctcta cgccctggcg
    11221 tcggctctct acgtcctgaa cctctaccgg ccgtccggat gggcgggccg gtcggcgacg
    11281 gcgtgcgccg tggtgggcgc cgtcttgaac ttcgcggcgc tctacgcccg ggcccttgcc
    11341 gtcggaaccg tcccctatcg cgacctcctg ggctcgatga agctcttcgg attcttcctc
    11401 gccgtcctct cggtcgtcct cgagctgcgc caccacgacc gcgcgctcgg cccggtcctc
    11461 atgccggcgg cgctcttctt catgctgctc gcgctcctcg aggcgccggg cggcccccga
    11521 cccgcgccga accccgacct caagggcgcg attttcgctc tgcacgtcac gctcaacatg
    11581 ctcgcgtacg ccgcgttcgc ggtctcggcc gccctgtcga tgctctacat cgccgcgggg
    11641 cgcgcgctga agtcgcacac gctctccggg cccgcgacgc gcctcccgac gctctcgtac
    11701 ctcgagcgcg cgaaccgcac gtcgctcggc ctcgggatca tcgcgctcgg ggcgggcctc
    11761 gcgctcggct tcctctgggc cgcgcgcgtg tggacgaacg agcaccccca ctgggggctc
    11821 gacccgaaga tcttcttcgc cctcggcacg ctcttcttct acctcttcgt cctcgtgcgg
    11881 gcgcgccgcg gcgcccccgg ggtctcgacg gcgcgcctct cgcttgcggg cttcgtgctc
    11941 gtgctcctgt cgtacaccgc cgtcaacatg ttcgtctcca agctgcacgt cttccgctca
    12001 tgaacctcgt cttcgtcggc tggaaccacc gcggtgcccc tctcgacctg agagagcgac
    12061 tcgccttcac gcccgaaaag gcgcgcgagg cgctcgaggg cctcttcgcc gagcgcatcc
    12121 tcgccgaggg cgcgatcgtc tccacgtgca accgcgccga gatctacggc gtgtccgagc
    12181 gcgaggacga cctggacgcg ctctccggtt acttctcgcg cttccaccgc gtggacgacg
    12241 tgctcctgcg ccagacggcg ctcacgggcc gcggcgacgc gacggtgagg cacctcttcc
    12301 gcgtcgcggc cgggctcgac tcgatggttc tcggcgaggc gcagatcctc gggcagattc
    12361 gcgaggcgca ccgcgtcgcg acggccgcgg ggtcgctgcg cgcggtcacg aaccggctct
    12421 tcatgaacgc cctcgagtgc ggcaagcgcg tgcgcgcgga gacgggcctc ggcacgcgtc
    12481 cgacgtccgt tgcgggcctc gccctcacgc tcgcgggccg catcttcgag tccctgagcg
    12541 gccgccgcgc gctcctcgtg ggcgccggcg agacggccga gctcacggcg cggctcctcc
    12601 tcgacgaggg cgtgtccgag atcgtcgtca cgaaccgctc gttcgaaagg gcgcaggctc
    12661 tcgcggcctc cgcgggaggc cgggccgtgc cctgggacca gcgcgccgtg gcggccgccg
    12721 aggtggacct catcctctcg gccacgaacg cgacggagcc cgtcctcacg accgccgcgc
    12781 tcaaggcgtc gctctccaac gcgaagcggc gcgggccgct cctcgtcctc gacctcgccg
    12841 ttccgcgcga cgtggaggtc gaggtgggcg aactgccgga cgtcttccgc tacgacctcg
    12901 acgccctcaa cgggatggcg gacgcgaacg cgaaggcgcg cctcgaggag gttccgaagg
    12961 ccgaggccat cgtcgaggag tgcgtccagc gtttcacgga ctggtggggc agcctcctgc
    13021 aggtcgacgt cgtgcgcggc ctccgggaga aggtcgagaa gatccggctc gccgagatcg
    13081 agaaatacgc gggcaagctg tccggcctgt ccgagaagga ccgcgccacg gtcgagcggc
    13141 tcacggagac gctcgtcggg cacatcctcc acgaacccac cgtcggcctc aaggagggcg
    13201 acgcgtccga gcgcctcgag aaggccgctt ccgtgaaagc gctcttccgg ctgggcgaac
    13261 ctccgaggtg acgacgatga cgaccgcacc ccctctccgc atgggcacgc gggccagcgc
    13321 gctcgcgcgc gcccagtccg gggccttcgc cgaggagctc acgcgccgct cgggccggcc
    13381 cgtcgaggtc gtgatcgttc ggacgcaggg cgaccagctt tcgagcgagg gcaaggcccc
    13441 gcccggcggc gactcggccg gcgtcttcgt gcgcgccctg gacgatgcgc tccggaaccg
    13501 cgagatcgac ttcgccgtcc attccctcaa ggacgtcccg acggccgacg ccgagggtct
    13561 cgtcctcgcc gccgtgcccg cgcgtgcgga ctcgcgggac gcgttcgtcg tcgcgacgcg
    13621 gcacgcggga gtgaagcgcg tcgaggacct cccgcagggc gcgaaagtcg cgacgtcgtc
    13681 gccgcgccgc gtctcgcagc tcctgcgcgt ccggccggac ctcgtgaccg tcgcgatggc
    13741 gggcaacgtg gacacgcgcc tccagcgcct cgaggaaggc cgcgccgacg cgctcatcct
    13801 cgcgagcgcg ggcctcgacc gcctcgggcg cggcgccgtc atcacgcgtc gtctcgagcc
    13861 ggacgagatg ctcggcgcgc ccgcgcaggg ggcgctcggc atcggctgcc gggcggacga
    13921 cgaggagacg aggatttctc tgctgaagat ggaagacccg atcgcacgag cggctgccgt
    13981 ggtcgaacgt gatctcctgc attttcttag aggtgggtgt cgcgctccgg ttggtgccag
    14041 tgcctcggtg gtgggcggcc ggctcgtttt gcgcggccgc gttctctccc tcgacgggcg
    14101 cgaatgcctc gaagacgaga tgagcgagcc ctacacgccg gcattccctt cttcaccttc
    14161 aaagaaatct tctctttctt cttctcttgg ctctctcctc gccgagaagc tgctcgcgcg
    14221 cggcgcggcg cggctcatcg acgcggcgcg cgtgtgagca ggcggatcct cctgctgcgc
    14281 tccggctcgg ccgggccgga ggattcggct gttgcgcccg atgtgacggt cctgcacacg
    14341 cacacggtcg aggctcggcc cgagggggtc gccgaagcgc tcgcgttcga cccggtcggc
    14401 gccacgctcg tcgtctcgtc gaaagagacc gtgagagttc tgctccctct cttcactcgc
    14461 cccttcgcct tgatggttgc ggttggcgag ggcacggctg aattgcttag aaaggtgaat
    14521 acggggggga ggccccgcgg cacttccggc gtgtcatcaa cctacagtgg ggctcgtgag
    14581 gcgcagatcg tggtgccggc gacacccggc gcggcgggcg ttattcacct tctaagagaa
    14641 tcttcggcgg cgtttccttc gcgcgtcctc tggccgcatg gctcggacgc tgcctccgaa
    14701 cccttcgaag aaatccgatc tctgggtttt tcgctcttgg cccccgtcgt ctatgtcaaa
    14761 cgcgctctct cttctctcga gctggatgcc ggcgtcgtcg ccgatttcgc cgccggccgg
    14821 tttggttcgg tggcggtcgg ttcgacggcc ggcctggacg tcctgctcgc ggccgtcccg
    14881 tcgccgccgc cggtaagatg gggcgtcctc gggcccgaga cggcgaaagc cgtcgccgcg
    14941 cgtaatcttc ccgctcccgt cgtcccgccg cgtgcccggc tctccgatct tctcgatctc
    15001 ctccggaagg aaccctcatg agttttcccc tccaccgcta ccgccgcctc cgccgcagcg
    15061 agagcctccg gacgatggtc cgcgagacgt cgctcgctcc ttccgacttc atcgcgccgc
    15121 tcttcgtcgt gcccggcaag ggcgtacgcc agggcgtctc gtccatgccg ggtgtggacc
    15181 gcacgtccgc cgagctggcc gccaaggacg cgaaggcgct cgccgacctc ggcgtcctct
    15241 ccgtgatcct cttcgggatc ccggaacaca aggacgcgaa gggcacgtcg tcggccgatc
    15301 cggagggccc cgtctgccag gccgtgaagg cgatcaaggc cgagagcccg aagacggtcg
    15361 tcatgacgga catctgcctg tgcgagtaca cggaccacgg gcactgcgga gtgatcaagg
    15421 gcgacacggt cgacaacgac ccgacactcg caatcctcgc ggccgaggcc gtcgcgcacg
    15481 cgaaggcggg cgcggacgtc gtcgcgccga gcgacatgat ggacgggcgc gtgggcgcga
    15541 tccgcgccgc gctggacaag gaggggttcg cggacgtcgc gctcctgtcg tacgccgcga
    15601 agtacgcctc cggcttctac ggcccgttcc gcgaggccgc cgagtcgacg ccgtccttcg
    15661 gcgaccggcg cgcgtaccag atggacccgg gcaacgtccg cgaggcggtc aaggagatcc
    15721 tctcggacgt ggccgaaggg gccgacatgg tcatggtcaa gccggcgctc tcgtacatgg
    15781 acgtcatccg cgccgcgaag gaagccacga acgtcccgct cgcctgctac aacgtctcgg
    15841 gcgagtacgc gatggtcaag gcggccgcgg cgaacggctg gatcgacggg cagcgggtca
    15901 cgctcgagat cctgaccagc ttcaagcgcg cgggggccga cctcatcctg acgtaccacg
    15961 cgaaggaagc ctcccagtgg atcggatgag gaagaggaag aaagagaaag agaagagagg
    16021 agaaagattt tcttagaagg gtgagtgggg ccgcggccac actcgacgcc ccggcccatg
    16081 tcgggcgcgc ggcgcccggt ttcaccttca aagaaaatac agcctgtcga tgagcgagaa
    16141 gaaagcaggt tctgcgcgcc cggtccgtag gcgtcttcgc cgaacgaccc aatccgaagc
    16201 tctctttctc cgtgcgaaga agcggatgcc cggcggcgtt tcgtcgcccg tgcgctcgtt
    16261 cgcctccgtc ggcgggacgc cgttcttcgt gaagaagggc aagggggcga cgctcgtcga
    16321 cgccgacggc aaccgttacg tcgactgggt gatgtcgtac gggccgctca tgtttgggca
    16381 cgcctacggg ccggtcctct cggccgtgcg caaggcgctg ccgcgcggca cgtcctacgg
    16441 cgcgccgacg aaggccgaga tcgagctggc cgagcgcgtc gcgaaggccg tgccctcgat
    16501 ggagaaggtg cgcttcgtct cgtcggggac ggaggccgcg atgagcgccg tgcgcctcgc
    16561 gcgcgccgcg accggccgcg acctcgtcct gaagttctcg ggctgctacc acggccacgc
    16621 cgactcgttt ctcgtttcgg ccggctcggg gctggcgacg ctcggcatcc ccggttcccc
    16681 gggcgttccg gcgtcgctcg ccgggatgac ggtcacggcc cggtacaacg atctcgattc
    16741 cgtgaaggcg ctcttcgcgg cccacccgaa acgcatcgcc gtgatcgcgg tcgagcccgt
    16801 cgccgcgaac atgggcgtcg tgacgccgga ggcgggcttc ctcgaggggc tgcgcgcgat
    16861 cgcggacatg gaaggggctc tcctcctctt cgacgaggtc atcagcggct tccgcctcgc
    16921 gaagggaggc gcgcaggaga tctacgggat caggcccgac ctcacgtgcc tcggcaagat
    16981 catcggcggc ggcctgccgg tcggcgcgta cggcggccgg gcggacctca tggcgcaggt
    17041 cgcgccggac gggcccgtct accaggccgg gacgctttcc ggaaacccgc tcgcgatggc
    17101 ggccggcaag gcgatgctcg acgcgctgac gcccgcggtc tacgcgaagc tggaccggac
    17161 cgcgcgcgat ctcgagcacg ggatgaacgc cgccgcgaag aaggcgggcg tcgcggaccg
    17221 ggtcgtcctg aaccgcatcg gctcgatcct gacgccgttc ttcacgaagg ggcccgtgcg
    17281 cgacttcgac gacgcgaaga aggccgacct cggcaagttc agggtctggt accacgcgat
    17341 gctccggcat ggcgtcttcg tcgcgccggc gcagttcgag gcgatgttcg tctcgacggc
    17401 gcacggcccg aaggaggtcg cgaagaccgt caaggcgcac gagatcgcgc tgaaggaagc
    17461 gttcgccgcg gcgtgacgcc gcccgacgtc tccgagagcc tgcgccgcga ggcgctcgaa
    17521 cgggaccgcg aggacccgct cgcgcgtttc cgcgccgagt tcgtcgtcgc ggacgagggc
    17581 ctcgtctacc tggacggcaa ctccctcggc cgcctcccga agaagacgga agctctcgtc
    17641 gcgaaggccg tgcgcgagga gtggggtgga cgcctgatcc gcggctggaa cgagggctgg
    17701 gtggacgcgc cccgccgcgt cggcgacaag ctcgcgcggc tcctcggcgc gcggccgggc
    17761 gaggtcgtcg tctcggactc cacgagcgtc aacctcttca agctggcggt cgcggcgctg
    17821 aaggcgcgcg aggcccggag caccatcgtc acggacgacc tgaacttccc gtccgacctc
    17881 tacgtgctgg acggcgcggc gcggctcctc ggcggcgggc ggcgcgtcgt cgccgttccg
    17941 gcccgcgacg gcacctccgt cgatcccgcc gacctcgcgg ccgcgatcgg gccggacacc
    18001 gccctcgtct cgctctcgca cgccgcgtac cggagcgggt tcctccacga catgagagcg
    18061 gtgacggaga tcgcgcaccg ggccggcgcg ctcgtcctgt gggacgtgag ccactcggcg
    18121 ggcgtcgtcc cgatcgacct cgaggcctgg ggcgtcgacc tcgcggtggg ctgcacgtac
    18181 aagcacctga acggcggccc gggcgccccc gcgttcctct acgtgcgccg cgagctgcag
    18241 acgtcgctcg aaaaccccgt cccgggctgg ttcgggcggg aggccccgtt cacgttcgcg
    18301 ccggacttcg cgccggcggc gggggtggag cgtttcctcg tggggacgcc gccgatcctc
    18361 tcgctcgcgg ccgcggaagc cggcgtcgac ctcgcgctcg aggccggcgt ccccgcggct
    18421 cgcgcgaagt cgctggccat ggggagattc ctcgaggatc tctggagagc catgctcgag
    18481 ccgaaaggcg tctcgctgcg ttcgccgtcg gagcctgcgc ggcgtggatc gcacctgtcc
    18541 ttcggacacc ccgaggctct tgcgatcgac cgtgcgctca tcgccgaaat gaacgtgatc
    18601 ccggacttcc gcccgcctga cgtgatccgc ttcggcttcg ccccgctcta caccactttc
    18661 gaagaactct tcgagggcgt ggcccggttc cgaggcgtgc tcgatgagga acgctggagg
    18721 cgtttcgcgg gggaggcggg gcccgtcgtc acctgacggc ccggtgcgtc actcgaacca
    18781 cccgacgacg tcgaggagga tgtgcgcggt acccgtcggg aggccgtcca cgacgacgtc
    18841 cccggcggct ccgagcggca tcgtgacgtt gttcgcgcgg gtctgaccgg cccggaaggc
    18901 cacggtcgcc gtgcccgtcg gcggcgcgcc gcccggcacg aggcgaaccg accccgcggc
    18961 cgtggatccc gtgaccgtga gatttccggc gatcgacttc gcggtggccg ggatgccgca
    19021 ttgcccgcgg atcgggaacg tccggacctg gcccgcgtac agcgtcgggc cgccgtacgt
    19081 tccgttcggc tttcgcgtgt cgaagacccg gcagggctgc aggacgtgga agctcgtggc
    19141 gggcggcggg gtcccgtagt cgacctggac cgtgagcgac gacgcggtct tcgagagcgc
    19201 cgtgatcgtg agggacagct cggggtccgt gaaggacttg ccgacgtcga gctgggcgtc
    19261 gtcgaagctg ccggcggtcg cgaagttcat gtcgaggagg tccgcgccgc cgacgctgct
    19321 cggggcgaag cggatcaatc ctccgttcgc gacgccggac ggctggccgg cgtcgaagcc
    19381 cgtgccgccg cgccactcga tccagtacgt gcggtcgctg cggctcgtcg ggatccgaac
    19441 cgcgtacgtc gattgtccgg gcgcttccag cgcgccgatc gtgtacgtcg ccgtcccgcc
    19501 cccgtggttc ttcacgctgc cccccggcag ccagccgaac gtgtccttga actgcgcgtt
    19561 gaagtgcccg gcgcgcgagt tgcccatcgt gtcgaagcgg tcgccgtact cgctgcgcgt
    19621 gcagcccgac ggcgcgatcg ccgcggaggg gcacgtgtac gagtgcgcgt gcacgacccc
    19681 gaagttgtgc ccgagctcgt gcgccgcgac gagcaggccc cacgagccgg tcggcgcgcc
    19741 tttgatccac gagccgccgc ctcccacgta cccgaggccg ctccagccgc aggcgctgat
    19801 cgacgggaag acccagacgg ggaagttgta gttcgcgggg ttgtagccgg cggcgagcgc
    19861 ccgcgcgttt gcctggctct ggatcgtgcc cgtgtcgcac gtcgtgggct tcgccaccgt
    19921 cgccgtcacg aggacgacgt cccccgtcat cgtcgtgagg ccgaaggagg cctcggcgta
    19981 gtagcgcgcg accgaggagc ccgcgccgaa gaacagggtc tgcgtgttcg tgaccgtcgt
    20041 cgcgttgaag gaggagtcgt ccgtgaagtt caggagcatc acgagcaccc gcttggggcc
    20101 ggtcgtccag gcgctcaggg ccgtccgtcc gagggccgac cgacccggtc caagggaatg
    20161 tatctcgtcg acctcgaagg cgcggcgccc gggccagccc gtgaccgaga cccggtctcc
    20221 ggttcggacg tcgggcgggg tggagaaacg gaggggcagg acgtctccct cttccgtctc
    20281 cagctcatag acattgctgg acttagaggc cgcgaagtcg tcctcgacga ggtgccgaat
    20341 gacgcccgtg cgcgtgaacg catcgtcccc ggacaccggc cgagcggcgg cgacgacggc
    20401 cgcgagggcg gccgcaacca ggagaaatcg ggcgcgaggc atcggggcct ccggaggttg
    20461 gaccagcctg tgtgcattcg gtagtgccgg ggcctaaccc ggagcctcgc cgaggggcct
    20521 cggcgggagc gttttcggag accccttgtc cctccgggga atttgatgca actgaatcac
    20581 agccttgggg atgcgcactt tcataccctg cgtcacggat gcaaaaggtc tttttctacg
    20641 cgcttaccga actcacgttc gtcaacacgc cgattctttc ccggcaagga ggctgaaatg
    20701 tccatggttt cccgcacggc cctcacgttg ctggccggcc tcatcgcggt tccgttcact
    20761 ccggtgaacc gcacggccac caacaacgcc cccgaacagc aggccgctcc cgcggcctac
    20821 gcggcgaccg ccaaggaggc gtaccttgcc cccgaagcgc tcacgtacat ccgtcccggg
    20881 ttcaacatca agatccaggc ggtgacgaac ttcgcggcgg gccagaagcc gaccgtggag
    20941 ctctacttca cggacgatct cggcgggccg ctggatcgcc tcggcctcgt gacgccgggc
    21001 accatctcgg tgcggttcat cccggcggtc tgggacccgg cgacgaggcg gtacacggac
    21061 tacctcggct acaacgcgga ccccgcgaag aacgcgaacc cgacccgtga caacgggacg
    21121 acgggcacct ggcaggagct cgaggtcggc cactacaagt acacgttcgg aaacgccttc
    21181 gcgcttccgg cgacgatgga cgcgacgaag ccccacacgt tcgcggcgat gggcgtccgc
    21241 aacacgaccg acatcgtcgg gaagccctac tacgcggtcc cgaagtatct cgacttcgtc
    21301 ccgtccacga acacggcggc ggcgacgttc aacgcgatgg gcgtcgcgcg ctgcaaccag
    21361 tgccacgacc cgctcgcgct gcacggcggc aactaccgcg acatcaagac ctgcgtcctc
    21421 tgccacaacc cgaacaacat gcgggggaac atcaacccca ccgagccgcg cgagcagttc
    21481 gacggccaga tcttcttcca cgccctccac gcgtcgaaga acgagctgat cggcgagatc
    21541 acgtacccgc aggacctccg cttctgcacg acgtgccacg acccggcggc ggccggcggc
    21601 gcgagctggt acacctaccc gtccgtcgcg gcctgcggct cctgccacga cgacgtgaac
    21661 ttcacgacgg gcgccaacca cgtcgccggc cccgcggccg acggcacctg cacgacctgc
    21721 cacaagccgg agggcagcac ggactgggac gcgggcatca agaacgccca cgtcgttccg
    21781 atccagtcga agcagctcaa gggctacaac gcgaagatcc tgtcggtcgc gaatgccggc
    21841 cccggcaaga agatctccgt gaccttccag ctcacgaacg gcgacggctc gttcgtcgac
    21901 ccgggggtct acaaggattc gacgaagggc agcatcagca tcaagctcgg cggcccgacg
    21961 accgactatg cgaacgtcgg catggccgcg aacggccagc ccttcagtga agccgcccag
    22021 accgccacgt acaacgcgac gaacggccag gccgtgtaca ccttcacgaa cgccatcccg
    22081 gccgcggcga agggcacgta cgtcgtctcg atcgacaccc gccgccccta cacgctgagc
    22141 cccgccccga agaacgggcc gacctcgatc aacgagggcg ccccgaacca gcccttctac
    22201 ttcgcggtca cgggcacgac ggtcaccccg cgccggacgt ccgtcgcgct cgcgaagtgc
    22261 aacgtctgcc acgcgaggct ggacaacctc ttctcgcacg gcggccagcg catcgagacg
    22321 cagcactgcg tcatctgcca cgccccgaac gccaccgacc tcggacgccg cccggcggac
    22381 ggctcgcaga accccggcga gtccatcgag ttcgcgcgga tgatccaccg gatccactcg
    22441 ggtgaagagc tgacccagga ctacacgatc tacgggttcg gcggcaacgc ggcgaacttc
    22501 aacgaggtca cgtaccccgg cgaccgccgg aattgcctcg cctgccacgt gaacgccgcg
    22561 gcgtactcga tcccgctccc gaacaccaac atcccggtcg tcacggagcg cgactactac
    22621 tcgccgcgcg ggtcgaccgc cgccgcctgc acgggctgcc acgactcccg cgacgtcgcc
    22681 gcccacgcgt acatcaacac ggcgtacttc ccgaacgccc cgacggtccc cgtggattcc
    22741 tgcggcacgt gccacgcgac cggagcggac gccggcgtcg acaaggtcca cgcgcgctaa
    22801 agaaggaagg cctgtgaaac acctctcgct cggagttgcc ttcgccgccc tcgccgcatt
    22861 cgcggcgggg tcggcggcgg cccaggcgcc ggccaagccg gcggcgaagg tggaggccgg
    22921 gccctcgtgc gccgactgcc acgacgaggc caaaatcttc gccaccaacc cccattcccg
    22981 tggctacgcc gccgcccaca agggcgcgac gggcaacgcg gtctgcgaaa cgtgtcacgg
    23041 cgacggcaag aagcacatgg aggcgggcgg cgacaagtcg ctcatcaaga cgctccatgg
    23101 aggccgctcg accgacacgt gccagacctg ccacaagcag tccgcggagc gcgcgtcctt
    23161 ctggaacagc gtgcacgcgt cgtccgaggc ggtgaattgc ctctcctgcc actccgtcca
    23221 caagtcgggc tacaagcaga aggctctcct gaagtccgac cccaaccagc tctgcgcgac
    23281 ctgccaccag gacgtgaagg cctcgttcac ggccatgccg tattcccacc gtctcaagga
    23341 cggcgtgatg tcgtgcgtga gctgccacga tcctcacggc cggccggcga accacgccgt
    23401 ccgacagacc cgcgcgggcg agccgggctg cgtctcctgc cacgcggaga agaagggccc
    23461 gttcgtctat ccccacgtca ccggcatcgc cggcagctgc tcgtcgtgcc accgtccgca
    23521 cgggtctccg tacccgcacc agctgacgag ggccacggtc tacacccagt gtctcgagtg
    23581 ccactccacg ctctcgacgg gcacgctggg atcgcagccg ccttccttcc acaacatctc
    23641 gctgccgcgc taccagaact gcacgacgtg ccacacggcc gtccacggtt cgcagcgcga
    23701 cccgcagctc ctgaagtaag gacaggccga tgcgaattct ccgctccgcc ttcctcacgg
    23761 ccgccctcac gttggtgctc gcctccggcg ttgcccgcgc ccaggcgatc cccttcgaca
    23821 tcgaggccgg ctatcgcttc ctctccgtga ccggaaacga ggagatgtac cgctcgcaga
    23881 tcaacgaccg cgagggcttc ctcatccgtt cgctccacgt cgggaccgac gagaagtacg
    23941 cgggcttccc gctcacggac aggttccggc tcgacgccgc cgacctcggc gccggcggcg
    24001 ccggcttcct gcgcttcgac gccgggcttt ccggcgtcta ccggctcagg ttctcgtacc
    24061 gcaacacgca gtacttcagc gccatccagg gcttcgcgaa cccgctcggc gccacggtcg
    24121 gccagcagac ctgggaccgc acgcgcaacc agttcaacgt cgacctcgag gtccttccgg
    24181 gcaaggtggt cacgccgatc gtcggctaca cctcgaacac cctgtcgggc cccgggtaca
    24241 cgacgtacca cttcagccag gacgagttcc agctctcgca gaacctgcgc agcacagacc
    24301 aggagttccg ggtcggcgcc gcgttcaacg cgggcgtcgt gtccggcgag gtcatccagg
    24361 gctggcggaa gtaccacgag accgaagacc tcaccctcgc cccgttcggc ggctcgggca
    24421 acaacacgtc gcccgtgctc ggcaagaacg tctcggccac ctcgctcacg cgctcgagca
    24481 ccacggacgt caacgtgccc gtcacgtcgg cgttcgtccg cggattcgcg ctgccgaacc
    24541 tccagatctc gggcttctac gtccggtcga acgcgacggg cgacgacggc acgaaggaag
    24601 gcgcctcggg cagcttcgcc tcgttcgcga tctcccggtt cttcacgggc tactcggcca
    24661 acaacacgac ctccccgaac agcctcacgt ggcgggcggg cggccgtgcg gagtggcacg
    24721 tcatgagcgg cctcgacgtc tcggcgggtt acacggagcg ggaccagacc tgggacaacc
    24781 aggccctgct caacgagctc tataccggca cgtcgacgtt cacgggcttc tcgcagtccg
    24841 atttccagtc cctcctctcg acgcagacca acatcgagcg caaggacaag gtgctggacg
    24901 tccaggcctc catgcgcttt ctcggaccgg tcggcgtccg cctcggcttc tcccgcatcg
    24961 accaggacct gaccgtcgtc gaggatccgg cggagatcgt cgtccccggc aaccagggcg
    25021 gcacgttctc ccgcagcatc aaccgctacg agggcgccct cacgttcacg atggccggct
    25081 tcaacatcat gggcgaggcc tcgttcgacg acgcgaacgc cgcgatcacc cgcgtggact
    25141 acctcaagcg caatcgcgag cgcgtccgtc tcacctggaa gaccttcgac tggatccagc
    25201 tgggcggcgc cgcgatgtgg ctcgaccaga agaacgagga ctcgggcccg ctcaacacct
    25261 acacggcgag ctcggccaac tactcggtgg acctcaccct caagccggtc aagtgggctt
    25321 ggctgcgcgg cgcctacggc aagctcgagg ccgacagctc catcccgatc cgcgttcccc
    25381 agaacttcac ggtcgtcaac tcggtgaaca cggaagacgg cgagctgatg gacttcggcg
    25441 ccggcttcaa gttcaaggag ttcgcggtcg acgggttctg gagccgcttc gacaacacgg
    25501 gctcctacct gttccggatg taccgcggcg gcgcgcgcgc ggactggacc gcctccgcgc
    25561 acgccggtct cgtggtcgag tggaccgtcg accggtacct cgacctcatg cagtcgacgc
    25621 agagctaccg cggcgaccgg ctcggtgttt acctgaggtg gcggccctga gaggcgccga
    25681 tttcgattgg aaggaaagtg aaatgaagag aatcaccctg gccgttttcg ccctcgtggc
    25741 ggccgtcacg ctcgtcgcgg gagcggcgtt cgcgacgatg gacctccaga aggagttcct
    25801 cgcgaagtac ccggactcca agatgaagtg cggctcctgc cacacgaagg cgatgcccaa
    25861 gaagggcgat gccgaggtca acaagttcgg cgccgacatg aacaaggtca agaaggacgg
    25921 caagtacgac ttcgcggccc tggacaagat ggacagcgac ggcgacggcg tttccaacgc
    25981 cgacgagctc aagaagggga cgaaccccgg cgatcccgcc tccaagtaac atgatgcgga
    26041 tgaggctcgg agtcgttctc accgcgttcc tgctctcggc gaccccgctc agagccgcgg
    26101 ccccgacccc gaaggccccg tccaacgacg actgtctcgg ctgccacgag gacccgtccg
    26161 ccaagagagc ggacgggtcc tctcttttcg tcgacaagag ggtcttcggt gaatccgtgc
    26221 acggcgccgc cggagcggcc tgcgtcgact gccacaccga tctcgccaag acgaaggact
    26281 tcccgcaccc cgagaagctc gcgaagcccg actgctcagg ctgccacgcc cagcaggtcg
    26341 aggactacgg gaagagctgg cacgcggccg cgcgggccaa gagcccgacg agcaaggccg
    26401 cctggtgcgc cgactgccac ggatcccacg acatcaagcc ttccaaggac ccggactccc
    26461 ggacgaacca cttcaacctg ccgaatacgt gcatgcgctg ccacggcgat ccgaagatct
    26521 tcccgcgtgc gggggggacc gacggcaccg agccggtgag cttccacgac tcgatccacg
    26581 gcaaggccct ggagaaatcg gggctcaagg tcgccccgaa ctgcgcgacc tgccacgggt
    26641 accacgagat ccgcagcccg aagcacgacg cgaagagcac cgtcgcgcgg gcgcacgtgc
    26701 cgtcgacctg cggctcgtgc cactcgaaga tcctcgacga gtattcgacg tcgatccacg
    26761 gcgagcagct cgcgaagggc aacgagagcg tgcccgtctg ctccgactgc cactccgccc
    26821 acgcgatctc ggcggcgccc gaggtgcggc gcctgaccat cctgaagcag tgcgggggct
    26881 gtcacgactc gtccctcaag agctaccggg acacctatca cggccaggtc tcggagctcg
    26941 ggttcgcggc ggtcgcgacc tgcgcggact gccacacggc gcacgagatc cacccggcgg
    27001 gcgacgtccg gtcgtcggtc gggccggcga agcggctcgc gacgtgccag aagtgccatc
    27061 cgggcgccgg gccgaagttc gcaaaatacg acccccacgc gaaccctcac gacaggggcc
    27121 gcagcggaat cctctggttc acggccctgt tcatgaagta cctcctgatc ggcgtcttcg
    27181 ccttcttcgg ggtccacacg gccctgtggt tcccgcggtc cgtcaaggcg cggcgcgagg
    27241 cggagcgccg cggcggaaac ggagacgcgc gatgaaggac cacgggaccg ccggtccggc
    27301 cgccgacgcc tacgtccgtc ggttcgacga gctcgaccgc cgcctccaca tcttcctcat
    27361 gacgagcttc ctcgggctcg cgctgacggg gcttccgctc ctgttctccg agcgcaagtg
    27421 ggcgaaggtg ctctcgcatc tcttcggcgg cttcaaggcg gcgggcggcc tccaccgctg
    27481 cttcgcggtc gtcatggtca tcgtcttctt cgtccacctc tcgcggatga tgaagaggct
    27541 gttcctcgac aaggacacga cgatcctctg gggtcccggc tcgatggtcc cgcagccgcg
    27601 cgatctctcc gagatgctcg ggcacatcag gtggttcgtg ggcctcggcc cgcggccgaa
    27661 cttcgaccgc ttcacgtact gggagaagtt cgactactgg gccgtcttct ggggcatggg
    27721 gatcatcggc ggctcgggct tcatcctctg gttcccgaag ttcttctcgc gcgcgctgcc
    27781 cggcgactgg ttcaacgtcg ccctcctcgt ccacggcgaa gaggcgctgc tcgcgaccgt
    27841 gttcatcttc acgatccact tcttcaacgg gcacctccgg cccgagaagt ttccgatgga
    27901 cacggtgatc ttcacgggcg tcctgccgct cgaggaggtg aagcacg
//
LOCUS       DAOMFY010000020        27747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_560_length_27747_cov_7.458905, whole genome shotgun sequence.
ACCESSION   DAOMFY010000020 DAOMFY010000000
VERSION     DAOMFY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27747
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_27747_cov_7.458905"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLB01_03295"
     CDS             complement(<1..991)
                     /locus_tag="PLB01_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HQQ76355.1"
                     /translation="MAAGKVLAAIDVGSNSIKLLVARRDPDDPGHAFEVLREKEMVRL
                     GQQTLAEGALSEEAMEDGLDCLARYAALARAAGAESIAAVATCAVREAANGGEFVRRA
                     RRETGVRLAVISGEEEARLITRAVRADLPPSCDPILVLDIGGGSTEVVVATGEKVLLA
                     ESLDLGAVRLTDLFVSSDPLSPHDEKALRKAVRSRLARLEKPVAKARIKTAVGTSGTI
                     LAVAEIAAVLNGREGAGAGHRSLSRRDLKKVVDLLVSSTLKQKARIGGLDADRADIVT
                     AGAILLQELFRAFGIETLLASERSLRDALVLESPQPARRRPGERNVRKESVRRL"
     gene            1035..2342
                     /locus_tag="PLB01_03300"
     CDS             1035..2342
                     /locus_tag="PLB01_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HQQ76356.1"
                     /translation="MAAFPSDRPRLWGVAVSHYQVEGGDACDWTAWEEAGRTKGGPCG
                     RAAGSWERYEEDADLAADAGANAFRFSVSWSRVEPARGTYDAAALARYGRLVDRLLER
                     GLEPIVTLHHYTHPAWFHAETPWTSPASVEAFARFARRVAEALAPRVRIWVTLNEPLV
                     LLLGGFLDGQIPPGLADGRAAGLALGNLYAAHAAAAATLKDVDARAAVGLAHNMMDFV
                     PSRPRWILDRLLAAHARHLYNRSFLEAFRTGTWDLWIPPFTRLHGRLPSLKRSLDFVG
                     VNFYSRFHVECPAPTRVLTRFSYRDPRGRGLTDNGWEIVPESFGPLLAEAGALGLPVL
                     VTENGLADGDDVRRSRFLQEHVAALLDAERAGVKVAGYLHWSLVDNFEWLDGYGPKFG
                     LYALDHGTLERRPRPSVAVFREIGKSFLAQELPEKRAQKPVHI"
     gene            2415..3194
                     /locus_tag="PLB01_03305"
     CDS             2415..3194
                     /locus_tag="PLB01_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQQ76357.1"
                     /translation="MLLADFHIHSTWSDGRLAIPEIVDAFGRTGHDAIAITDHVVNED
                     SLLGKIAHSCRLSITKDNWDAYRAEIERETKRAWDEYRMVVLAGVELTRNALTGKRSA
                     HALALNLERFVSADGPVEEMLTRAREAGAITVACHPNEQSDWFANTFYIWNRRKELAE
                     LVDLWELACRWDLFPQVSRARLPYLGNSDFHDMPHLWAWKTLMQCEKTPEAVVATLRS
                     GLGLGVTRLLAPSPKAVRTPSETQPLEPCSVIPGFVQPATA"
     gene            3202..4422
                     /locus_tag="PLB01_03310"
     CDS             3202..4422
                     /locus_tag="PLB01_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ76358.1"
                     /translation="MTLTLALLVLAAAGALMTTFQTGLVLRFLGRKRPFAPGPRERAA
                     SAFRPAGSTATPRVSVLKPLCGLDDGLEQNLASFAALTGVTYEVILSAARADDPAVAV
                     ARKVIRRFPATAFRLVVGGGTGVALANPKVDRLVAASREAHGEILLVSDSNVRVHPGD
                     VARIAAAFDDPEVGCVSSLFTGSEARSFGAVVESLHLLTFVLPGAIIAEFGGVPCVVG
                     KSMALSRSALEAIGGFAAFVDVLAEDQAIGCAVRDAGLKVALSPVVVRNVTTHRPLER
                     ALARQVRWGKIRYSFSKLTYTGELLLNPFPIALLACAAAALLAPRALPPAALLAGVLL
                     LLRLFQGFALAQATHADHSAGQLLLLPVKDVLQLFTQAAPYFSKEVDWQGHRARLGPG
                     TLLLPSRHAAAAAA"
     gene            4548..6668
                     /locus_tag="PLB01_03315"
     CDS             4548..6668
                     /locus_tag="PLB01_03315"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HQQ76359.1"
                     /translation="MHLSLRRNLLFSLASVALAATLAGLSPRASADLTLRVTPPNKVQ
                     LLQNQRFDIRVEATADAGATVTSLKVTVDGIDVTSRGVSSGFAQYSNWDFRYAEFGIS
                     GNRVIAATATSSNGQTLSASSTVTVRAWDQSQIQRPSGVATNNLVGIGTFPDVEAAAG
                     RSSFARAAREVGIRDVFADADEMHLGKVSPLFHVYPNTAEARAAGLVDVPVKKAKNVI
                     LFIGDGMGAAHRTAARVMSKGYTAGKANGTLAMDRLPYNGLHMTSSLNSLITDSAPGA
                     HNYSTGNKTNNGMEGVFPDNTGPNDDNPRIENLSEFSWRMFGKVTGIVSNAFLTDATP
                     AAFIAHTADRGDGTLVANQYFDQRNTNGLKVLMGGGAYHFIPKSTTGSRRTDERNVVN
                     DFKNDGWTYVDTATALNGFAPGPNGRLFGLFNLDNMNVAFDILKQGDPSVTATFPDQP
                     LLKELTQKAIDTLKQYPNGFFLMVESAHIDKQAHRMDAERSIYEVIQLDHAVQVAMDF
                     AAANGETLVIVSADHECAGMALPAVGRPDKKGTQDYAKSYNYAKVGAGANDSAISNFT
                     DYVVGTNGYPVSPEPSRRLIVNFGAAPEHYEDWTYSKLPRNSSATEGGGFDPGIVSGG
                     KAVANPNDPKKNTAGAFIVSGVLETGASGGDAQTQAVHTMSDIPISAMGPGASQFARV
                     QDNTEAFFEIMNAMLGTFPIPASF"
     gene            6670..7149
                     /locus_tag="PLB01_03320"
     CDS             6670..7149
                     /locus_tag="PLB01_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76360.1"
                     /translation="MRRLVAAALLCAAAPLSAADALPHLTWNDFFAKVASTGATFSDH
                     VKELDGRRVVIRGYAVVDPQPPGGLYLTRFPEGKLHPDDEDTLPWDSVGVVWKKGTRL
                     PSIPKQPSIEGTLRLGNRDLGTETVILVLEDAVPHVDKAAKKAATTAARPSASGGPR"
     gene            complement(7109..7471)
                     /locus_tag="PLB01_03325"
     CDS             complement(7109..7471)
                     /locus_tag="PLB01_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQQ76361.1"
                     /translation="MSETIDLFRNAEETETYPAGHVIFREGDLGHAMYAVQDGEVDLV
                     VHGNVVETVRPGGIFGEMSLLLEGHRRSATAVARTDCRLAPVTERRFNFLVQQTPMFA
                     LQVMRIIAGRLMRMDARL"
     gene            7525..8022
                     /locus_tag="PLB01_03330"
     CDS             7525..8022
                     /locus_tag="PLB01_03330"
                     /inference="COORDINATES: protein motif:HMM:NF027443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4833 domain-containing protein"
                     /protein_id="HQQ76362.1"
                     /translation="MKALRTLLLAFLAASPARGDETCGPLFVIARSLNANVVLYEARF
                     RADGTLDAEKPLTATWRLDAKDGRRESLNFVERIRAYGFDLSPLDDRDRWQVKVRALP
                     TRPLVLHAGTGCPFVTADVGGRDAILRRVYVTATGGLLPKVASVELSGVDRETALPVT
                     ERFTP"
     gene            complement(8080..11076)
                     /locus_tag="PLB01_03335"
     CDS             complement(8080..11076)
                     /locus_tag="PLB01_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQQ76363.1"
                     /translation="MTNLSRNVVRALVVALAVVTAGGALAQSQATTGVIEGIVSDSGG
                     VPLPGVSVAIRNTATNFEKTAVTGADGRFRGLALPLGPYRVTATLKGFATLVREGFDL
                     GVGQTINLTLPLTLSQKAEAITVTAAAPVIETARTEGSVQIGTEAIRGLPNNGRNFLE
                     FTKLTPGVSIVQGPDGDELTVNGQKGIQNNVSVDGNDFNNPFFGEQRGGQRPAFTFNM
                     DAVKEVVVVSDGANAEFGRASAGFVNVVTKSGTNEMHGSVHGFYKDDNLSSAAVKADG
                     SSAEKYDFSQLQTGFTLGGPFIKDKLFFFTAFDYQKGKSTKQTDPNRIDPALVSYFAS
                     VGAPGQNAPIDRTNDARVFLGKIDWAVNEKHLATVRYNYTWSEQENGTFDVDPWATSS
                     NAIEQDHSNSVSGSLQSTLTNSVLNEFRFQWAREDRPRPYDGPQVPGGGRPFPDTGVD
                     FVKGYRFGMPFFIPVDYYDTRLQFNENLTYITGNHTIKAGLEYNRTNATQTFRGFGNG
                     RIIFGSVNGFLNFTKNSKYVECSNGTSNNTGNCPAGTSITGPVVLYLQHVPGPGLTID
                     QAGTQSIVQEEPAVFIQDKWQPLPNLTISFGLRWEAQIQPDPITPPESVFFAPFIGKT
                     SYGQEFPSDGKIPSDYAMWQPRLGITWDPTKDGKTVVRANGGIFAARVPGLNLASTRS
                     TNGSLGYDVYRDSSLTNILGPVPAYPNIIPLSSLPSTPFQPGVYVYSKDFSNPRTYSA
                     SLAIEREVMKDVALSLRYNYAHTVNIARFINRNDPLLGNGTVGPWTTGLPGGNGLGSL
                     TTVESTGHSVYNGITLGLQKRYATNWEIQAFYTLSWDKSDDDNERDPFSYRYAKVTDL
                     GPEYGYSDRDQRHRFNAYVLWKAPLDINVNGSWQYRSAQPQSITATGAIANTPQDRIN
                     PDGSVTERNLGRKDNQFNSFNLRISRVFMAGNVQIEPIFEVFNLFNSKNLRNPGSTSL
                     LFNFDGTVQSGLGDPRQAQFGVRVVF"
     gene            11293..12018
                     /locus_tag="PLB01_03340"
     CDS             11293..12018
                     /locus_tag="PLB01_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76364.1"
                     /translation="MTLTRSAALAVLGSAGACALLCGIPAPASLFAFPVALSLFQFCV
                     TPLLTNAGLYRYHSEMLKATLRNERTWEIHGGTSWDYVRLFRLSESGAVATRRLLRFY
                     LEGLLDIARLVESGVISPGTTITGTSYFFSEKSARRLGFRLEPAGPRLRAVLVLYYLD
                     LVLLYSFARGRIAFPNVLKARRAAIRAADLAARRPEIEALIARLDRPPAAPRAASRTS
                     YRLRNAQATCAKYPEGNPARPSP"
     gene            complement(11946..12713)
                     /locus_tag="PLB01_03345"
     CDS             complement(11946..12713)
                     /locus_tag="PLB01_03345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQQ76365.1"
                     /translation="MQLSPAEAWSLGAIAAAAIAFLVLERRVPYDPGQGVFRPGFFTD
                     FVWYNAVQSWALGVLIGRLIAAIDGVTGFSRLALVSGWPVLGQVGFFVVTHDLYIYAM
                     HRLQHRVPFLWRFHEAHHSVPQVDWLSGVRSHPVEILVNQTIEFAPIVLLGAAPAVAP
                     IKGAISAIWGMYIHSNLDVRMGALQRVLNGPEMHRWHHATDPEAQGRNFATKLAVWDW
                     LFGTAYLPGRKPTGYGLGRAGFPSGYFAQVAWAFRRR"
     gene            complement(12704..13630)
                     /locus_tag="PLB01_03350"
     CDS             complement(12704..13630)
                     /locus_tag="PLB01_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HQQ76366.1"
                     /translation="MEFVPRNPKREVVLRDREIGDGHGVYVVAEIGINHNGSLDIARQ
                     IIDGAARAGCDAVKFQKRTPELCVPRDQWDVERDTPWGRMTYLAYRHKVEFGWREYAE
                     IDRFCREKGIAWFASCWDEPSVDFIEQFDPPVYKAASASLTDHALLRRMKNTGKPLMI
                     STGMSTMDEIESAVASLGLTNLLVAHATSTYPCPAGELNLRMIPALRQKYPFTPVGYS
                     GHEPGLAPTVAAVAMGASFVERHVTLDRTLWGTDQAASVEIVGLERLVRDIRTVEEAL
                     GDGVKRVYDSELSSRRKLRRVFVEEAEQALCS"
     gene            complement(13655..14170)
                     /locus_tag="PLB01_03355"
     CDS             complement(13655..14170)
                     /locus_tag="PLB01_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQQ76367.1"
                     /translation="MAPDVVARASRVRLVLTDCDGVLTDGGVFYSAYGEEMVRFDRRD
                     GMGVERLRELAGIETGIVTRERSEIAARRAGKLGIHELHLGAFDKAGVLEDVARKRDL
                     TPSQVAFIGDDVNDLPALARAGLAGCPADAFEAVREACHYVTHAPGGHGAFREFAELV
                     LHAVLRETPRR"
     gene            complement(14203..15021)
                     /locus_tag="PLB01_03360"
     CDS             complement(14203..15021)
                     /locus_tag="PLB01_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ76368.1"
                     /translation="MTDRFSLAGRVAVVTGAAGLLGKRHACALASAGARLALGDVDGD
                     AADALARQIAAAHGVPALGLALDVTSRGSLLAFRDAVLGEFGRLDVLVNDAAVNDKFE
                     DPLLATELSRFESYPLTAWQRMIEVNVTGTFLACQAFGAAMAERGAGSIVNIASTYGL
                     VGPDQSLYVRPDGTRAFWKSPAYPAGKGAVLAFTRYLAAYWGPKGVRVNALSPGGVED
                     GQESWFVGNYARRTPLGRMAGPDDTAGAVLFLASDASAYMTGANLVVDGGFTAW"
     gene            complement(15018..16361)
                     /locus_tag="PLB01_03365"
     CDS             complement(15018..16361)
                     /locus_tag="PLB01_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ76369.1"
                     /translation="MPATALPLVAADPVTTESDRLWARAQGLIPAGTQTLAKGPGQHV
                     RGFAPKYLVSGKGARVWDADGSTYLDFTMGVGPLILGYAEPAVDDAIRLQLESGITFS
                     LMHPLEVEVAEAIRAAVPSVESVRFSKTGADATSAAVRLARAFTGRDKVVCCGYHGWH
                     DWYIGVTDRDRGIPQASKDLVHTIAYNDLESVADALDGDTACVILEPAVFEAPRDNFL
                     ARLKALCEANGTLLVFDEMWTGFRVALGGAQELFGVTADLVTYSKAVANGMPLSVLAG
                     RRDVMALLERDVFFFTTFGGEALSLAAAQATLHELRTRGVPAWLARQGRRVREGVNGL
                     AKLHGVSGFVRCAGLDARTIVTFDASAGNPLELKSFVQQELLRRGVLWHGFHNMSAAH
                     GEDEVSHLLAAYDEVFPLLKDAVEAGDVRARLKGDPVEPVFRRTTSFNTKPKARA"
     gene            complement(16372..17286)
                     /locus_tag="PLB01_03370"
     CDS             complement(16372..17286)
                     /locus_tag="PLB01_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQQ76370.1"
                     /translation="MSAATYETLLRDLALADPRIVVLTAENRAAIRGVPAALGERFVD
                     VGIAEQTLVGMSAGLALRGRLPVAHALATFLTMRAFEFIRTDVGIPGLPVKLVGAVPG
                     FLSEANGPTHQAIEDVALMRGIPGMKVFAPGDEQDLLLGLPHVLADPSPWYVRFNTRR
                     AAVPHAPFTPGRAEIVREGTDAAILTYGTLLTEAVGAAELLGEWGISCRLVNLRTLAP
                     IDAEAILAAAAETPLLVTLEDHFETGGLFSIVSELLVRRRVPAPVLPLALPARWFAPA
                     LLPHVLAEEGFQPIQIASRVREALARRS"
     gene            complement(17283..18134)
                     /locus_tag="PLB01_03375"
     CDS             complement(17283..18134)
                     /locus_tag="PLB01_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HQQ76371.1"
                     /translation="MNGGAEARERQALRVRERIIRLATDGGCFLGASLSCTDLLVALY
                     EDFLELGAGFEDPDRDVLLLSKGHDVPALYALFVERGWLPAARLARHLEPGSHVYWHP
                     NRNIPGVEFHSGSLGHLLSVGLGIALDARLRASRARVVVVLGDGELNEGSIWEGLLVA
                     KAHRLSNVLLVVDRNGFQANLRTEDLVPLEPLAAKFEAFGCAVRRTDGHDFGALASAL
                     EAFPYGSGGPSVLIADTVRGKGLPSIEGRLDRWFCNFTVGEVEALVEELHGADEARLV
                     TAGEVVR"
     gene            complement(18131..18943)
                     /locus_tag="PLB01_03380"
     CDS             complement(18131..18943)
                     /locus_tag="PLB01_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQQ76372.1"
                     /translation="MSGVVTVVQARTGSTRLPGKVLLPLAGAPGLLRMVERVRTARFS
                     GTVVVATTTHAEDDVIETLCRTHDLPVFRGHPTDLLDRHVRAARAFGAEHVVKIPSDC
                     PLIDPAAIDRVIGFFLADEESWDYVSNLHPATWPDGNDVEIMPLGALETAWREAVRAC
                     DREHTTPFLWDHPERFRVGNVAWEAELDLSATHRFTLDYAEDHAFLAEVYAALYPVKP
                     HFTLRDVLELLAERPALRAINARRLGTNWYARHLHELRTITPAEARSATEAA"
     gene            complement(18940..19941)
                     /locus_tag="PLB01_03385"
     CDS             complement(18940..19941)
                     /locus_tag="PLB01_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76373.1"
                     /translation="MRQTAPARKRVLLIGGSLNQTTMMHKIGERLAATCDCAYSPFYC
                     DQPLRSLQKAGLLDFTIFGGPMRRMTEEYLRSHTLPVDEGGVAADYDLVVTCSDLVLP
                     KNVRNQPLVLVQEGMTDPETFAYHLVRKANLPLWVASTSTTGLSLKYDKFCVASEGYR
                     EFFAAKGVPRAWIEVTGIPNFDDCASYLENEFPYRGHVLVATSDTRETFKRDDRRALV
                     DRALTIANGRRLVFKLHPNENHERSTREIRAWAPDALVFTSGNVNHMIANCEVLITQF
                     SSCVFVGLALGKECHSNFDIGELKKLVPLQNGRTSAANIAAVCEGLLAAPAARRLSA"
     gene            20124..20477
                     /locus_tag="PLB01_03390"
     CDS             20124..20477
                     /locus_tag="PLB01_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQQ76374.1"
                     /translation="MALNVETSGVVRVITLEGKLGTEIAQDLKTEFDAYLAGSPGPTL
                     LDMSQVRYISSYLVGVLVDMRSRLAARGYALHFAGIDARHRLVLTISGLDELFGYHAE
                     RAEGIAALAASAKPA"
     gene            20544..21998
                     /locus_tag="PLB01_03395"
     CDS             20544..21998
                     /locus_tag="PLB01_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQQ76375.1"
                     /translation="MPNPRALRRKIQELREDVSAEGDALAARWKPWVRRREFAFSALN
                     LAHYLALRRRDLRRLQEALMPWGLSSLGRCESRVLPTLDGVLASLDALCREQTPGVEH
                     PDPRAYFRGLRFLERRARRALGPAPPEHHVRVMVTLPGEAATEPGIAKALLERGASCF
                     RINCAHDGAEAWEAMGRHVRRAEQEIGRPARILMDLAGPKLRIAAVSERARLERGLRF
                     LLTREEPVPGASPVEASCAEPGVVETLRVGERVFVDDGSAVATVVSLEPRGALLEVTR
                     SKGKKLKPEKGLNFPDSDLRVPSLGSKDLADLDSILRIADLVGYSFVRSADDVALLQD
                     EMRARLPDAWRSKGIIAKIETPLAVRNLPDIIVRGAGSQPFGVMIARGDLAVELGFVR
                     IAEIQEELLWLCEAAHVPVIWATQVLEAFVKNGIPTRAEMTDAAMATRAECVMLNKGP
                     FVADAVHVLGRVLSRMEAHQFKKTPQLRALQAWR"
     gene            complement(22310..22615)
                     /locus_tag="PLB01_03400"
     CDS             complement(22310..22615)
                     /locus_tag="PLB01_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76376.1"
                     /translation="MKTKALTRLSPLEPALEAPLKELGIAVASLVPANEPDVSRDARS
                     IVRRLVPDPTKNPYLLDGVPKFVTSLLDSLNRLEPKYGAERAAIATAWNKVEGAAAS"
     gene            22721..23203
                     /locus_tag="PLB01_03405"
     CDS             22721..23203
                     /locus_tag="PLB01_03405"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HQQ76377.1"
                     /translation="MKKTPKTIGLVAHDNRKRDLIEWVNWNFTILVRHRLVCTGTTGR
                     LVEEALQAQLTAAKNPAPMPEITKLKSGPLGGDQQLGAMIAEGRVDVIVFFWDPMEPH
                     PHDVDVKALLRIAVVYNIPIACNRSTADFIISSPLLAQPYSPIVKDYSAHVDRTVQSE
                     "
     gene            complement(23157..24386)
                     /gene="coaBC"
                     /locus_tag="PLB01_03410"
     CDS             complement(23157..24386)
                     /gene="coaBC"
                     /locus_tag="PLB01_03410"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQQ76378.1"
                     /translation="MTIHIVLGVSGGISAYKSAEIVRALVEAGAEVRVLMTPAATRFI
                     TPLTLAVLSKNPVVADLWDASSGAVDHIALARWADVLAIAPATADVLAKLAHGIGDDA
                     LTTYALGHRKALVLAPAMNTWMWTHPATEENLGTLGSRGATVVEPDAGDLACGDVGPG
                     RLAPPARIARAILDAGVVSRSLEGRRVLVTAGPTREPVDPVRFLSNRSSGKMGYALAR
                     EAARRGADVVLVSGPVSLTPPPGARLVRVERTSEMREAVLAEIAEADVLLMAAAPADF
                     MVAVPAGEKIKRTAGIPNLELAPTPDVLREASGAARKGTVLVGFAAETQDLLANAARK
                     LAEKGVDLLVANDVSRPGIGFDADENEVVLLAPGTEPQRVERSSKLVVAGRILDRVAE
                     LLLTPTARSGPRAPSSP"
     gene            24520..25425
                     /gene="panB"
                     /locus_tag="PLB01_03415"
     CDS             24520..25425
                     /gene="panB"
                     /locus_tag="PLB01_03415"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQQ76379.1"
                     /translation="MSTVPASAARVTPRHILAKKARGEPVVMVTAYDHPSASAADAAG
                     VDAILVGDSLGMVVLGYDDTLRVTMDDMLHHARAVARARTAALRVGDMPFLSYQADVA
                     EAVRNAGRFLSDGGMDAVKLEGGRERAGTVRAIVEAGIPVMGHLGLTPQSIRALGGYR
                     VQGATAEAARRVLDDALRLEDAGCFAIVLEAIPDRVAALVTSRLAVSTIGIGAGAACD
                     GQVLVWHDLLGWEERIAPRFVRRYAAMAEEARVALGRYGAEVRERRFPAAEHVYPIAD
                     AEWARLQADLAARPPAARRPESVAW"
     gene            25419..26267
                     /gene="panC"
                     /locus_tag="PLB01_03420"
     CDS             25419..26267
                     /gene="panC"
                     /locus_tag="PLB01_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQQ76380.1"
                     /translation="MVIVRTPFEARRERRALGGSVGFVPTMGALHEGHLSLVRRARDE
                     NAHVAASVFVNPAQFGPREDLAAYPRALERDLGLLEREGVALVLAPEAGAMYPSGFET
                     WVAPGRLAERLEGERRPGHFRGVLTVVAKLFNAIEPDRAYFGQKDAQQAILIRRMARD
                     LDFAADIVVCPIVRERDGLAMSSRNVYLSREERRAASVLARALAAASVAWDAGERRGD
                     VLRRAALTVLATEPLANPDYVSAADPSTLQEMDGPADHLLISMAVRFGATRLLDNVVL
                     PHGTAL"
     gene            26279..27043
                     /locus_tag="PLB01_03425"
     CDS             26279..27043
                     /locus_tag="PLB01_03425"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQQ76381.1"
                     /translation="MLLAIDIGNTTITLGLYDGETPGPRWRLETVRTRLADEYGLALS
                     SLLATAGLTSADVGKAAMSSVVPPLTGTWTEVCRRTLRLEPLVVGAGVKTGVRVLYDD
                     PKAVGADRIVNAVAVKEMFGGPAIVVDFGTGTTFDALSAKGEYLGGAIAPGIGIAAEA
                     LHARAAKLPRVDLVRPPAAIGRNTEHAMQSGLLFGYVGLVEGMVARFRAELGGKAKVV
                     ATGGYAELVAKETKVLTTVAPWLTLDGLRLIAAMNR"
     gene            complement(27054..27701)
                     /locus_tag="PLB01_03430"
     CDS             complement(27054..27701)
                     /locus_tag="PLB01_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQQ76382.1"
                     /translation="MLAAFPLGLVCGFVGGFVGAIPPGPIGITCLRRSLLGEKRQAYG
                     VALGGASVDLLTCALIGFGVGWVIERVVTSPWVRGPLALFLVASGVKLLVDARGASVS
                     ATPSGAAAGAVGAGLPFLTGFLQGAANPGPFVNWTLFISFLIGHRLLDPSPAGAGGFA
                     LGVGLGVFSWFTLLIRAAHRLKDHSGPWIARSTAVAGAFLLATGLYFGWRSFAAA"
BASE COUNT         4249 a         9813 c         9495 g         4190 t
ORIGIN      
        1 cgaggcgccg gacggattcc ttccgcacgt tccgctcgcc cgggcgccgg cgggcgggct
       61 gcggcgactc gaggacgagc gcgtcgcgga gcgagcgctc ggacgcgagg agcgtctcga
      121 tcccgaacgc acggaacagc tcctggagga ggatcgcgcc ggccgtcacg atgtccgcgc
      181 ggtccgcgtc gaggccgccg atgcgcgcct tctgcttgag cgtcgaggac acgaggaggt
      241 ccacgacttt cttcaggtcg cggcgcgaga gcgaccggtg gccggcgccg gcgccctcgc
      301 ggccgttcag gacggccgcg atctcggcca ccgcgaggat cgtgcccgac gtcccgaccg
      361 cagtcttgat ccgcgctttc gcaacgggct tctcgagccg ggcgagacgc gaacggaccg
      421 ccttgcgcag cgccttctcg tcgtgcggcg aaagcgggtc cgacgagacg aagaggtcgg
      481 tcagccggac ggcgccgagg tcgaggctct cggcgaggag gaccttctcc cccgtcgcga
      541 caacgacctc cgtcgagccg cccccgatgt cgaggacgag gatagggtcg caggacggcg
      601 gcaggtccgc acgcacggcc cgcgtgatga gccgcgcctc ctcctccccc gagatcacgg
      661 cgagccggac gcccgtctcc cgccgggcgc gccggacgaa ctcgccgccg ttcgcggcct
      721 cgcggaccgc gcacgtggcc acggcggcga tggactcggc gccggccgcc cgtgcgagcg
      781 ccgcgtaccg cgcgaggcag tcgaggccgt cctccatggc ttcctcggag agcgcgccct
      841 cggcgagcgt ctgctgcccg aggcggacca tctccttctc gcgcaggacc tcgaacgcgt
      901 gccccgggtc gtccgggtcg cgccgcgcga cgaggagctt gatcgagttc gacccgacgt
      961 cgatcgctgc gagaaccttg ccggccgcca tcttcacgcg accttatcgc agcttctgta
     1021 atctcggttt gaagatggcc gcctttccgt cggaccggcc gcgcctctgg ggggtggccg
     1081 tctcgcatta ccaggtcgaa ggcggcgacg cctgcgactg gacggcgtgg gaggaagcgg
     1141 gccggacgaa gggcgggccc tgcgggcgcg cggcgggctc ctgggagcgc tacgaggagg
     1201 acgccgacct cgccgccgac gccggtgcga acgcgttccg gttctcggtc tcgtggagcc
     1261 gcgtcgagcc cgcgcgcggg acgtacgacg ccgccgcgct cgcgcggtac ggccggctcg
     1321 tggaccggct gctcgagcgc ggcctcgaac cgatcgtcac gctgcaccac tacacgcacc
     1381 cggcctggtt ccacgcggag acgccgtgga cgtctcccgc gtcggtcgag gccttcgccc
     1441 gcttcgcccg gcgcgtcgcc gaggcgctcg cgccgcgtgt gaggatctgg gtcacgctga
     1501 acgagccgct cgtcctcctc ctcggcggat tcctcgacgg gcagattcct ccgggtctcg
     1561 cggacgggcg cgcggccggg ctcgccctcg gaaacctcta cgcggcgcac gcggcggcgg
     1621 ccgcaacgct caaggacgtc gacgcgcggg ccgcggtcgg cctcgcgcac aacatgatgg
     1681 atttcgtgcc gtcgcgtccg aggtggatcc tcgaccgcct cctcgccgcc cacgcgcggc
     1741 acctctacaa caggtccttc ctcgaggcgt tccggaccgg gacgtgggac ctctggatcc
     1801 cgcccttcac gcgcctccac ggccggcttc cgtccctgaa gcgctcgctc gacttcgtcg
     1861 gcgtgaactt ctactcgcgc ttccacgtcg aatgccccgc gcccacgcgc gtcctgacgc
     1921 gcttctccta ccgcgacccc cgcggccgcg gcctcaccga caacggctgg gagatcgtcc
     1981 cggagtcctt cgggccgctt ctcgccgagg ccggcgctct cgggctgccc gtcctcgtca
     2041 ccgagaacgg cctcgcggac ggcgacgacg tccgccgctc tcggttcctc caggaacacg
     2101 tcgcggccct cctcgacgcg gagcgcgcgg gcgtgaaggt cgcggggtac ctccactggt
     2161 cgctcgtgga caacttcgag tggctggacg gctacggccc gaagttcggc ctctacgccc
     2221 tggaccacgg gacgctcgag cggcggccgc ggccctcggt ggccgttttc cgcgaaatcg
     2281 gcaagtcctt cctcgcgcag gaattgccgg aaaagcgtgc acagaaacca gtgcacattt
     2341 aacaggagcc aaacgggcga ttcactcgct ccccacttcc gggccccatc ttccgcgggc
     2401 aggagggctt cctcatgctc ctggccgact tccacatcca ctcaacatgg tcggacgggc
     2461 ggctcgccat cccggagatc gtcgacgcgt tcgggcgcac cggccacgac gccatcgcga
     2521 tcacggacca cgtcgtcaac gaagactcgc ttctcggaaa gatcgcgcac tcctgcaggc
     2581 tctcgatcac gaaggacaac tgggacgcgt accgcgccga gatcgagcgc gagacgaaga
     2641 gggcctggga cgagtaccgg atggtcgtcc tcgccggcgt cgaactgacg cgcaacgcgc
     2701 tcacgggcaa gcggtcggcc cacgccctcg ccctgaacct cgaacgcttc gtctccgccg
     2761 acgggcccgt cgaggagatg ctcacgcgtg cccgggaggc aggcgccatc accgtggcct
     2821 gccacccgaa cgagcagtcg gactggttcg cgaacacgtt ctacatctgg aaccgccgga
     2881 aggagctcgc cgagctcgtc gacctctggg agctcgcctg ccgctgggac ctcttcccgc
     2941 aggtttcccg cgcgcgcctc ccgtacctcg gcaacagcga cttccacgac atgccccacc
     3001 tctgggcctg gaagacgctc atgcagtgcg aaaagactcc ggaagccgtc gtcgccacgc
     3061 tgcgctccgg cctcggcctc ggcgtcacgc gcctcctcgc cccctccccg aaggccgtgc
     3121 gcacgccgag cgagacgcag cccctcgagc cctgctccgt cattccgggc ttcgtccagc
     3181 ccgcaaccgc atgaggacac catgacgctc acgctcgccc ttctcgtcct cgccgccgcg
     3241 ggcgcactca tgacgacgtt ccagaccggc ctcgtcctgc gcttcctcgg gagaaagcgc
     3301 cctttcgcgc cgggcccgcg ggagcgggcc gcgagcgctt tccggccggc cggttccacg
     3361 gcgacgcccc gggtctcggt tctcaagccg ctctgcgggc ttgacgacgg cctcgaacag
     3421 aacctcgcgt cgttcgcggc gctgacgggc gtcacgtacg aggtgattct ctcggccgcc
     3481 cgtgcggacg accccgccgt ggccgtggcg cgcaaggtca tccggcgctt cccggcgacc
     3541 gccttccgcc tcgtcgtcgg cggcgggacg ggcgtcgccc tcgcgaaccc caaggtcgac
     3601 cgcctcgtcg ccgcctcgcg cgaggcgcac ggcgagatcc tcctcgtctc ggactccaac
     3661 gtgcgcgtcc accccggtga cgtggcgcgg atcgccgcgg cgttcgacga ccccgaggtt
     3721 ggctgcgtct cgagcctctt cacggggtcg gaggcccgct ccttcggcgc ggtcgtggag
     3781 agcctgcacc tcctcacgtt cgtcctgccc ggggcgatca tcgccgagtt cggcggcgtg
     3841 ccgtgcgtgg tgggaaagtc gatggcgctc tcccgcagcg ccctcgaggc gatcggcggc
     3901 ttcgcggcgt tcgtcgacgt cctcgccgag gaccaggcga tcggctgcgc ggtccgcgac
     3961 gcaggtctca aggtcgcgct cagcccggtc gtcgtccgga acgtcacgac acaccggccg
     4021 ctcgagcgcg ccctcgcgcg ccaggtgcgc tggggaaaga tccgctattc gttctcgaag
     4081 ctcacgtaca ccggcgagct tctcctgaac ccgttcccga tcgcactcct cgcctgcgcg
     4141 gccgccgccc tgctcgctcc gcgagccctc ccccccgcgg ctctcctcgc aggcgtcctc
     4201 ctccttctcc gcctcttcca gggcttcgcg ctcgcgcagg cgacgcacgc ggaccactcg
     4261 gccggccagc tcctcctgct gccggtcaag gacgtcctcc agctcttcac gcaggccgcg
     4321 ccctactttt cgaaggaagt cgactggcag ggccaccgcg cgcggctggg ccccgggacg
     4381 ctcctcctgc cctcccgcca cgccgcggcc gcggccgcct gaccgggccc gggcccgtct
     4441 tcttacgaag tcttcacgca gaagacacgc ggagaagact tctgagaatg agattgcgtg
     4501 gcgtctcgca cgacacggct ctcgccgtcg aggcaaggag gatctccatg catctttccc
     4561 ttcgcagaaa tctcctcttc tccctggcgt ccgtcgcact ggccgcgacc ctcgcgggcc
     4621 tgtcgccccg cgcgtccgcg gatctcacgc tccgcgtcac gccgcctaac aaggtccagc
     4681 tcctgcagaa ccagcgcttc gacatccgcg tcgaggccac ggccgacgcc ggggcgacgg
     4741 tcacgtcgct gaaggtcacg gtcgacggca tcgacgtgac gagccggggc gtctcctcgg
     4801 ggttcgcgca gtactcgaac tgggacttcc gctacgcgga gttcgggatt tccggcaacc
     4861 gggtgatcgc ggcaacggcg acgagctcga acggccagac cctctcggcc tcgtcgacgg
     4921 tcaccgtccg cgcctgggac cagagccaga tccagcggcc gtccggcgtc gcgacgaaca
     4981 acctcgtcgg catcggaacg ttccccgacg tcgaggccgc cgccggacgg tcgtccttcg
     5041 cgcgcgccgc ccgcgaggtg ggcatccgcg acgtcttcgc cgacgccgac gagatgcacc
     5101 tcggaaaggt ctctccgctc ttccacgttt acccgaacac ggccgaggcc cgggcggccg
     5161 gcctcgtgga cgtgccggtc aagaaggcga agaacgtcat cctcttcatc ggcgacggca
     5221 tgggcgccgc gcaccgcacg gcggcgcgcg tgatgtcgaa gggttacacg gccggcaagg
     5281 caaacggcac gcttgcgatg gaccgtctgc cgtacaacgg tctccacatg acgtcctcgc
     5341 tcaactcgct catcacggac tccgcccccg gcgcccacaa ctactccacg ggcaacaaga
     5401 cgaacaacgg gatggaaggc gtattcccgg acaacacggg cccgaacgac gacaacccgc
     5461 gaatcgagaa cctctccgag ttctcctggc gcatgttcgg caaggtcacc gggatcgtgt
     5521 cgaacgcgtt cctcacggac gcgaccccgg ccgccttcat cgcgcacacg gccgaccgcg
     5581 gcgacggcac gctcgtcgcg aaccagtact tcgaccagcg caacacgaac ggcctcaagg
     5641 tcctcatggg cggcggggcc taccacttca tcccgaagtc cacgacgggc tcgcgacgca
     5701 ccgacgagcg caacgtcgtc aacgacttca agaacgacgg ctggacgtac gtcgacacgg
     5761 ccaccgcgct caacggcttc gcgcctggac cgaacggccg cctcttcgga ctcttcaacc
     5821 tcgacaacat gaacgtcgct ttcgacatcc tcaagcaggg cgatccctcg gtcacggcga
     5881 cgtttcccga ccagcctctc ctgaaagaac tgacgcagaa ggccatcgac acgctcaagc
     5941 agtacccgaa cggcttcttc ctcatggtcg agagcgccca catcgacaag caggcgcacc
     6001 gcatggacgc cgagcgttcg atctacgagg tcatccagct cgaccacgcc gtccaggtcg
     6061 cgatggactt cgccgccgcc aacggcgaga cgctcgtgat cgtctcggcc gaccacgagt
     6121 gcgccggcat ggcgcttccg gccgtcggcc gcccggacaa gaagggcacg caggattacg
     6181 caaagtccta caactacgcg aaggtcggcg ccggcgcgaa cgactcggcg atctcgaact
     6241 tcacggacta cgtggtcggg acgaacgggt atcccgtgag ccccgagcct tcccgcaggc
     6301 tcatcgtgaa cttcggcgcg gcgcccgagc actacgagga ctggacgtac agcaagctgc
     6361 cgcgcaactc ctcggccacg gaaggcggcg gcttcgaccc ggggatcgtc tcgggcggaa
     6421 aagccgtcgc gaacccgaac gaccccaaga agaacacggc cggcgcgttc atcgtctcgg
     6481 gcgtcctcga gacgggcgcg agcggcggtg acgcgcagac gcaggccgtc cacacgatgt
     6541 cggacattcc gatctcggcg atgggccccg gtgcgtcgca gttcgcgcgc gttcaggaca
     6601 acaccgaggc gttcttcgag atcatgaacg cgatgctcgg gaccttcccg atcccggcgt
     6661 cgttctgaca tgagacgact ggtcgcggcg gcgctcctgt gcgccgccgc tcccctttcg
     6721 gcagccgacg cccttcccca cctcacctgg aacgacttct tcgcgaaagt agcgtccacg
     6781 ggcgcgacgt tctcggacca cgtgaaggag ctcgacggca ggcgtgtcgt gatccggggg
     6841 tacgcggtcg tcgacccgca gccgcccggc gggctctacc tcacccggtt cccggagggc
     6901 aagctccacc cggacgacga ggacacgctg ccctgggact ccgtcggcgt cgtctggaag
     6961 aagggcacga gactcccctc gatcccgaag cagccttcga tcgagggcac gcttcgcctc
     7021 gggaaccgcg atctcggcac ggagacggtc atcctcgtcc tcgaggacgc ggttccccac
     7081 gtcgataagg cggcgaagaa ggcggcgact acagccgcgc gtccatccgc atcaggcggc
     7141 ccgcgatgat ccgcatgacc tggagcgcga acatcggcgt ctgctgaacg agaaagttga
     7201 accgccgctc cgtcaccggg gccagccggc agtcggtcct ggcgaccgcc gtggcgctgc
     7261 ggcggtgtcc ctcgagcaga agcgacatct cgccgaagat cccaccgggc cggacggtct
     7321 cgacgacgtt cccgtgcacg acgaggtcga cctcgccgtc ctgcacggcg tacatcgcgt
     7381 gcccgagatc tccctcgcga aagatcacgt gcccggccgg atacgtctcg gtttcctccg
     7441 cgttgcggaa cagatcgatc gtttcgctca tcgcccctcg tcctccgtcg tgcctacaat
     7501 gtcggagatt gccgtgagat ccgtgtgaag gcgcttcgca ccctcctgct cgcgttcctc
     7561 gccgcctccc ccgcccgcgg ggacgagacg tgcggcccgc tgttcgtgat cgcccggagc
     7621 ctcaacgcga acgtcgtcct ctacgaggcc cgtttcaggg ccgacggcac gctcgacgcg
     7681 gagaagcccc tgacggccac ctggcggctg gacgcgaagg acggccgccg cgagagcctg
     7741 aacttcgtcg agcggatccg cgcctacggc ttcgacctct cgccgctgga cgaccgcgac
     7801 cggtggcagg tgaaggtccg ggcgctgccg acccggccgc tcgtgctcca cgcgggaacg
     7861 ggctgcccgt tcgtcacggc agacgtcgga ggccgggacg cgatcctccg ccgcgtctac
     7921 gtgaccgcga cgggaggcct cctcccgaaa gttgcgtccg tcgagctctc cggcgtcgac
     7981 cgggagacgg cgctccccgt caccgagcgc ttcacgccct gagccctgaa aaatgcggcg
     8041 gagcgcggcc caccccgcgc cccgccgatg gtcggggctc tagaagacga cgcgcactcc
     8101 gaactgcgcc tgccgcgggt cgccgaggcc gctctgtacc gttccgtcga agttgaagag
     8161 cagggacgtg gacccggggt tcctcaggtt cttgctgttg aagaggttga agacctcgaa
     8221 gatgggctcg atctgcacgt tgcccgccat gaagacgcgg gagatccgga ggttgaacga
     8281 gttgaactgg ttgtccttcc ggccgaggtt gcgctccgtg acgctgccgt cggggttgat
     8341 gcggtcctgg ggcgtgttcg cgatcgcgcc cgtcgccgtg atcgactgcg gctgagccga
     8401 gcggtactgc cagctgccgt tcacgttgat gtcgagcggc gccttccaga ggacgtaggc
     8461 gttgaaccgg tggcgctggt cgcggtccga gtacccgtac tccgggccca ggtccgtgac
     8521 tttcgcgtac cggtacgaga acgggtcgcg ctcgttgtcg tcgtcggact tgtcccacga
     8581 gagggtgtag aaggcctgga tttcccagtt cgtcgcgtag cgcttctgga ggccgagcgt
     8641 gatcccgttg tagacgctgt ggcccgtcga ctccaccgtc gtgagggacc cgaggccgtt
     8701 ccccccggga agcccggtcg tccacggccc gaccgtcccg ttcccgagaa gaggatcgtt
     8761 gcggttgatg aaccgcgcga tgttgaccgt gtgcgcgtag ttgtaccgca gggagagggc
     8821 gacgtccttc atgacttcgc gctcgatcgc gaggctggcc gagtacgtgc gcgggttcga
     8881 gaagtccttg ctgtagacgt agacgccggg ctggaacgga gtcgagggaa gcgaggagag
     8941 cgggatgatg ttcggatagg ccggcaccgg gccgaggatg ttcgtgagcg agctgtcccg
     9001 gtacacgtcg tagccgaggc tgccgttcgt cgaccgcgtg gacgcgaggt tcagaccggg
     9061 gacgcgcgcc gcgaagattc cgccgttcgc gcggaccacg gtcttgccgt ccttcgtcgg
     9121 gtcccaggtg atcccgaggc gcggctgcca catcgcgtag tccgagggaa tctttccgtc
     9181 ggacggaaac tcctgcccgt acgacgtctt cccgatgaac ggcgcgaaga agaccgactc
     9241 gggcggcgtg atcgggtcgg gctggatctg cgcttcccag cgcaggccga agctgatcgt
     9301 gaggttcggg aggggctgcc acttgtcctg gatgaagacc gcgggctctt cctggacgat
     9361 gctctgagtg cccgcctggt cgatcgtgag acccgggccc ggcacgtgct ggaggtagag
     9421 gacgacgggc ccggtgatcg acgtgcccgc ggggcagttt cccgtgttgt tcgacgtgcc
     9481 gttcgagcac tcgacgtact tgctgttctt cgtgaagttc aggaacccgt tgaccgagcc
     9541 gaagatgatg cggccgttcc cgaacccgcg gaacgtctgc gtcgcgttcg tccggttgta
     9601 ctcgaggccg gccttgatcg tgtggttgcc cgtgatgtac gtcaggttct cgttgaactg
     9661 cagccgcgtg tcgtaatagt cgacggggat gaagaacggc atcccgaacc ggtagccctt
     9721 cacgaagtcg acgcccgtat ccgggaacgg ccggccgccg cccgggacct gcgggccgtc
     9781 gtacggacgg ggcctgtctt cccgcgccca ctggaaccgg aactcgttga ggaccgagtt
     9841 cgtgagcgtg gactggagcg agccgctcac cgagttcgag tggtcctgct cgatcgcgtt
     9901 cgagctcgtc gcccacgggt cgacgtcgaa cgtgccgttt tcctgctcgg accacgtgta
     9961 gttgtagcga accgtcgcga ggtgcttctc gttgacggcc cagtcgatct tcccgaggaa
    10021 gacgcgcgcg tcgttcgtcc ggtcgatcgg tgcgttctgg ccgggcgctc cgacggacgc
    10081 gaagtacgac acgagcgccg ggtcgatgcg gttcgggtcg gtctgcttcg tcgacttgcc
    10141 cttctggtag tcgaacgcgg tgaagaagaa gagcttgtcc ttgatgaagg gcccgccgag
    10201 cgtgaagccc gtctgcagct ggctgaagtc gtacttctcg gccgaagagc cgtcggcctt
    10261 gacggcggcg gacgagaggt tgtcgtcctt gtagaagccg tgcacgctgc cgtgcatctc
    10321 gttcgtgccg gacttcgtca cgacgttcac gaagcccgcc gacgcccgac cgaactcggc
    10381 gttcgcgccg tccgagacga cgacgacttc cttcacggcg tccatgttga acgtgaaggc
    10441 cggcctctgt ccgccgcgct gctcgccgaa gaacgggttg ttgaagtcgt tgccgtcgac
    10501 ggagacgttg ttctggatgc ccttctggcc gttgaccgtg agctcgtcgc cgtcggggcc
    10561 ctgcacgatc gaaacgccgg gcgtcagctt cgtgaactcg aggaagttgc ggccgttgtt
    10621 cgggaggccc ctgatcgcct cggtgccgat ctggacggag ccttccgtcc gcgccgtctc
    10681 gatgaccggc gccgcggccg tcacggtgat ggcctcggcc ttctgcgaga gcgtgagagg
    10741 gagcgtgagg ttgatcgtct ggccgacgcc gagatcgaag ccctcgcgca cgagcgtcgc
    10801 gaagcctttc aacgtcgcgg tgacgcggta cggcccgagc gggagcgcca ggccccggaa
    10861 acggccgtcc gcgcccgtga ccgcggtctt ctcgaagttc gtcgccgtgt tccggatcgc
    10921 gacggagacg cccggcagcg gaacgccccc ggagtcggac acgatgcctt cgatcacgcc
    10981 cgtcgtggcc tgtgactggg cgagagcgcc gccggccgtc acgacggcca gggcgacgac
    11041 gagggcacgg accacgttac gcgagaggtt cgtcatcaaa aaccctcctg caagagttga
    11101 ggaaaaaggt atgggtagtg gttcggtgct ttcgagacgc aacctattgg ggctcttacg
    11161 caaagtcaat gaggaaaacg tgtaaagatt cagtgaagac tcggtcgcaa ccgaagtgcg
    11221 gcgcgagcag catcacgcgc ccgagacgcg cccctcacgc ggatctgagg tgcatccggc
    11281 accttccggg cgatgacgct gacgcgctcg gccgccctcg ccgtcctcgg ttcggccgga
    11341 gcgtgcgcac tcctgtgcgg aattccggcc cccgcttccc tcttcgcctt tcccgtcgcc
    11401 ctctcgctgt tccagttctg cgtcacgccg ctcctgacga acgccggcct ctaccgctac
    11461 cactccgaga tgctgaaggc gacgctccgc aacgaacgca catgggagat ccacggcggc
    11521 acgagctggg actacgtccg gctgttccgg ctttccgaga gcggcgccgt cgcgacgcgc
    11581 cgcctgctcc gcttctacct cgagggcctg ctcgacatcg cgcggctcgt cgagtccggc
    11641 gtcatctctc ccgggaccac catcaccggc acgtcgtatt tcttcagcga gaagagcgca
    11701 cgccggctgg gcttccggct cgagccggcc ggtccgcggc tccgcgccgt gctcgtcctc
    11761 tactacctcg acctcgtgct gctctacagc ttcgcccggg gccggatcgc cttcccgaac
    11821 gtgctcaagg cccggcgcgc ggcgattcgc gccgcggacc tggccgcgcg ccggccggag
    11881 atcgaggccc tcattgcgag gctcgatcgt ccgccggcgg cgccacgcgc cgcctctcga
    11941 acctcttacc gcctccggaa cgcccaggcc acctgcgcga agtatcccga gggaaatccc
    12001 gcgcggccga gcccatagcc ggtcggcttg cggccgggca gataggccgt gccgaagagc
    12061 cagtcccaga cggccagctt cgtcgcgaag ttccgccctt gcgcctcggg atcggtcgcg
    12121 tgatgccacc ggtgcatctc cgggccgttc aggacacgct gcagcgcgcc catcctcacg
    12181 tcgaggttcg agtggatgta catgccccag atcgcgctga tcgcgccctt gatcggcgcg
    12241 acggccggcg ccgcgcccag caggacgatc ggcgcgaact cgatcgtctg gttcacgagg
    12301 atttcgaccg ggtgcgaccg aacgccgctc agccagtcga cctgcggcac cgagtgatga
    12361 gcctcgtgga acctccacag gaacggcacg cggtgttgca gccgatgcat cgcgtagatg
    12421 tagaggtcgt gcgtcacgac gaagaaccca acctgcccga ggacgggcca gccggacacg
    12481 agcgccagac gggagaagcc cgtcaccccg tcgatggccg cgatgagccg gccaatcaac
    12541 acgcccagcg cccagctctg gacggcgttg taccagacga agtccgtgaa gaacccgggg
    12601 cgaaagacgc cttgccccgg gtcgtacggg accctgcgct cgaggacgag gaaggcgatc
    12661 gccgcggccg cgatcgcccc gaggctccac gcctccgccg ggctcagctg cacagcgcct
    12721 gctcggcctc ctcgacgaac acccggcgca gcttccgacg cgacgaaagc tcgctgtcgt
    12781 aaacgcgctt gacgccgtcg ccgagcgcct cctcgaccgt ccgaatgtcg cggacgagcc
    12841 gctcgaggcc gacgatctcg acggaggccg cctggtccgt gccccagagc gtgcggtcga
    12901 gcgtcacgtg gcgctcgacg aacgaggcgc ccatcgcgac ggccgccacc gtcggcgcaa
    12961 gccccggctc gtgcccggag tacccgaccg gcgtgaacgg gtacttctgg cggagcgcgg
    13021 gaatcatgcg gagattcagc tccccggccg ggcaggggta ggtcgacgtc gcatgcgcga
    13081 cgagcaggtt cgtgaggccc agcgaggcga ccgccgactc gatctcgtcc atcgtcgaca
    13141 tgccggtgga gatcatgagc ggcttgccgg tgttcttcat cctgcgcagc agcgcgtggt
    13201 cggtcagcga ggccgacgca gccttgtaga ccggcgggtc gaactgctcg atgaagtcga
    13261 cggacggctc gtcccagcag gacgcgaacc aggcgatccc cttctcgcgg cagaagcggt
    13321 cgatctcggc atactcgcgc catccgaact ccaccttgtg ccggtacgcg aggtacgtca
    13381 tccggcccca cggcgtgtcg cgctcgacgt cccactggtc ccgcggcacg caaagctccg
    13441 gcgtgcgctt ctggaacttc acggcgtcgc aacccgcgcg ggccgcgccg tcgatgatct
    13501 gtcgcgcgat gtcgagggag ccgttgtggt tgatcccgat ctcggcgacg acgtagaccc
    13561 cgtgcccgtc gccgatctcg cgatcacgca ggacgacttc tctcttcggg ttgcgcggga
    13621 cgaactccat ggggggctcc tttcgggtgc cgcgtcagcg acgcggcgtt tcgcggagaa
    13681 cggcgtggag gacgagctcg gcgaactccc ggaaggcccc gtggccgccg ggcgcgtggg
    13741 tcacgtagtg gcaggcttcg cggacggcct cgaacgcgtc ggccgggcag cccgcgagac
    13801 cggccctcgc gagggccgga aggtcgttca cgtcgtcccc gatgaaggcc acctgggacg
    13861 gcgtcagatc ccgcttgcgg gcgacgtcct cgaggacgcc ggccttgtcg aatgccccga
    13921 ggtgcagctc gtggattccg agctttcccg cacgccgcgc cgcgatctcg gaccgctccc
    13981 gggtcacgat gccggtttcg attcccgcca gctcgcgcag gcgctcgacg cccatgccgt
    14041 cgcggcggtc gaaccggacc atctcctcgc cgtacgcgga gtagaagact ccgccgtccg
    14101 tcagaacgcc gtcgcagtcc gtgaggacga gccggacgcg cgacgctcgc gcgacgacgt
    14161 cgggcgccac ggctgcgaaa acacctcgcg aacgcgacgc ggtcaccacg cggtaaaccc
    14221 tccgtccacg acgaggttcg cgcccgtcat gtacgccgac gcgtcgctcg ccaggaagag
    14281 gacggctccg gccgtgtcgt cgggtccggc catccggccg agcggcgtcc gccgcgcgta
    14341 gttgccgacg aaccagctct cctgcccgtc ttcgacgccg cccggcgaga gggcgttcac
    14401 gcgcactcct ttcggccccc agtacgcggc gaggtagcgc gtgaaggcga gaacggcccc
    14461 tttccccgcc gggtatgcgg gcgacttcca gaacgcacgg gtcccgtccg ggcgcacgta
    14521 cagcgactgg tcggggccga cgagcccgta cgtggacgcg atgttcacga tgctgcctgc
    14581 gccccgttcg gccatcgccg ccccgaaggc ctggcacgcg aggaacgtcc ccgtgacgtt
    14641 cacctcgatc atccgctgcc acgccgtgag cggatacgac tcgaaccgcg acagctccgt
    14701 ggcgagcaac ggatcctcga acttgtcgtt cactgccgcg tcgttgacga ggacgtccag
    14761 ccggccgaac tctccgagaa cggcgtcgcg gaaggcgaga aggctccccc tgctcgtgac
    14821 gtcgagggcg agcccgagag cggggacgcc gtgcgccgcg gcgatctgcc gcgccagcgc
    14881 gtccgcggcg tcgccgtcca cgtcaccgag cgcgagcctc gcgccggccg aggccagagc
    14941 gcaggcgtgg cgctttccga gaagccccgc ggcccccgtc acgaccgcca cgcggcccgc
    15001 gagcgagaac cggtccgtca cgctcgcgcc ttcggcttcg tgttgaagga cgtcgtccgg
    15061 cggaacacgg gctcgacggg atcgcccttc aggcgcgcgc gaacgtcgcc cgcctccacg
    15121 gcatccttca gcagcggaaa gacctcgtcg tacgccgcaa ggaggtggga cacctcgtct
    15181 tccccgtgcg ccgcgctcat gttgtggaac ccgtgccaga ggactccccg ccggagcagc
    15241 tcctgctgaa cgaagctctt gagctcgagc ggattccccg ccgaggcgtc gaacgtgacg
    15301 atcgtccggg cgtcgaggcc ggcgcagcgg acgaagccgg agacgccgtg gagcttcgcg
    15361 aggccgttga cgccttcccg cacgcgccgt ccctgccgcg cgagccacgc gggcacgccg
    15421 cgcgtccgga gctcgtgcag ggtggcctgc gccgccgcga gcgagagagc ttcgccgccg
    15481 aacgtcgtga agaagaagac gtcccgctcg aggagcgcca tcacgtcgcg ccgtccggcg
    15541 aggacggaca gcggcatccc gttcgcgacc gctttcgagt acgtgacgag atccgccgtg
    15601 acgccgaaga gctcctgggc gccgccgagg gccacgcgga atcccgtcca catctcgtcg
    15661 aagacgagaa gcgttccgtt cgcttcgcaa agggccttca gacgcgcgag gaagttgtcg
    15721 cggggcgcct cgaagaccgc cggctcgagg atcacgcaag ccgtgtcgcc gtccagcgcg
    15781 tccgcaacgc tctcgaggtc gttgtaggcg atcgtatgga cgaggtcctt cgaggcctgc
    15841 gggatccccc ggtcccggtc cgtgacgccg atgtaccagt cgtgccagcc gtggtagccg
    15901 cagcagacga ccttgtcgcg gcccgtgaac gcgcgcgcga gccgcacggc cgccgacgtc
    15961 gcgtccgcgc ccgtcttgga gaaccgcacg ctctcgacgg acggaaccgc cgcgcggatc
    16021 gcctcggcga cctcgacctc gagcgggtgc atgagcgaaa acgtgatccc cgattcgagc
    16081 tgcagccgga tcgcgtcgtc caccgcgggc tccgcgtagc cgaggatgag cggtcccacc
    16141 cccatcgtga agtcgaggta ggtgcttccg tcggcgtccc acacgcgcgc gccctttccg
    16201 ctcacgaggt acttcggcgc gaagccgcgg acgtgctgtc cgggcccctt cgcgagggtc
    16261 tgagtgcccg cggggatcag gccctgcgcg cgggcccaca ggcggtccga ttccgtcgtg
    16321 acggggtcgg cggcgacgag cggaagggcg gtggcgggca cggggcctcc ttcaggaacg
    16381 gcgggccagc gcttcgcgga cccgcgaggc gatctggatg ggctggaacc cctcttcggc
    16441 gagcacgtgg ggcaggagcg ccggcgcgaa ccagcgcgcc gggagcgcga gcgggaggac
    16501 gggcgccggg acgcggcgtc gcacgaggag ctccgacacg atcgagaaca gcccgcccgt
    16561 ctcgaagtga tcctcgagcg tgacgaggag cggcgtctcg gccgcggcgg cgaggatcgc
    16621 ctccgcgtcg atcggtgcga gcgtccggag gttcacgagg cggcacgaga tgccccactc
    16681 cccgagcagc tcggcagccc cgaccgcttc cgtcagaagg gtcccgtacg tcaggatcgc
    16741 ggcgtcggtg ccctcgcgca cgatctccgc gcggccgggc gtgaacggcg cgtggggcac
    16801 ggccgcgcgg cgcgtgttga agcgcacgta ccacggcgaa gggtccgcga ggacgtgagg
    16861 cagcccgagc aggaggtcct gctcgtcccc gggggcgaac accttcatcc cgggaatgcc
    16921 gcgcatgagg gcgacgtcct cgatcgcctg atgcgtcggg ccgtttgcct cggacaggaa
    16981 accgggcacg gcgccgacga gcttgaccgg caggccgggg attccgacgt cggtccggat
    17041 gaactcgaag gcgcgcatcg tgaggaacgt cgcgagcgcg tgggcgacgg ggagccgccc
    17101 gcgcagcgcg aggcccgccg acatcccgac gagcgtctgc tcggcgatgc cgacgtccac
    17161 gaaacgttcc cccagcgccg cgggaacgcc gcggatggcc gcgcggttct cggcggtcag
    17221 gacgacgatg cgcggatcgg ccagcgcgag atcgcggaga agggtctcgt acgtcgccgc
    17281 gctcaccgga ccacctcgcc cgcggtcacg agccgggcct cgtcggcccc gtgaagctcc
    17341 tcgacgagag cctcgacctc ccccaccgtg aagttgcaga accagcggtc caagcggccc
    17401 tcgatcgacg gaaggccctt cccgcggacg gtgtcggcga tgaggacgct cgggcctccc
    17461 gagccgtacg ggaacgcctc gagcgcggaa gcgagggcgc caaagtcgtg gccgtcggtt
    17521 cggcgaacgg cgcagccgaa cgcctcgaat ttcgcggcga gcggctcgag cggcacgagg
    17581 tcctcggtcc gaaggttcgc ctggaatccg ttccggtcca cgacgaggag gacgttcgag
    17641 aggcggtggg ccttcgcgac gaggagccct tcccagatcg atccctcgtt cagctcgccg
    17701 tcgccgagga cgacgacgac acgcgcgcgc gacgccctca gccgcgcgtc gagggcgatg
    17761 ccgaggccga ccgacaaaag gtgcccgagc gagcccgagt ggaactccac gccgggaatg
    17821 ttccggttcg ggtgccagta gacgtgcgaa cccggctcga ggtgccgggc gagccgggcc
    17881 gcggggagcc agcccctctc cacgaagagc gcgtaaagcg ccgggacgtc atgccccttc
    17941 gacagcagga ggacgtcccg gtccggatcc tcgaagcccg cccccagctc gaggaagtcc
    18001 tcgtagagcg ccacgagcag gtcggtgcag gaaagcgacg ctccgaggaa gcacccgccg
    18061 tcggtcgcga gccggatgat ccgctcgcgc accctcaggg cctgtcgttc ccgggcctcc
    18121 gctccgccgt tcacgcggcc tccgtcgcgg accgcgcctc ggccggcgtg atcgtgcgca
    18181 gctcgtgaag gtggcgcgcg taccagttcg tcccgaggcg gcgggcgttg atcgcgcgga
    18241 gggcgggccg ttccgcgagc agctccagaa cgtcgcgcag ggtgaagtgc ggcttgacgg
    18301 gatagagcgc ggcgtaaacc tccgcgagga acgcgtggtc ttcggcgtag tcgagcgtga
    18361 accggtgcgt tgccgagagg tcgagctcgg cctcccacgc cacgttcccc acccggaagc
    18421 gctccgggtg gtcccagagg aacggcgtgg tgtgctcccg gtcgcacgcg cgcaccgctt
    18481 cgcgccatgc cgtctcgaga gcgccgagcg gcatgatctc gacgtcgttc ccgtccggcc
    18541 acgtcgcggg gtggaggttg ctcacgtagt cccagctctc ctcgtccgca aggaagaacc
    18601 cgatgacccg gtcgatcgcg gccgggtcga tgagcgggca gtcggacggg atcttcacaa
    18661 cgtgctcggc gccgaacgcg cgggccgcgc gcacgtggcg gtcgaggaga tcggtcggat
    18721 ggccccggaa gaccggcaag tcatgggtgc ggcagagggt ctcgatcacg tcgtcctcgg
    18781 cgtgcgtcgt cgtcgcgacc acgacggtgc ccgagaagcg ggctgtgcgg acgcgctcga
    18841 ccatccgcag caggccgggc gcgcccgcca gcggcaggag aaccttgccc ggaaggcgcg
    18901 tcgacccggt gcgcgcctgc acgacggtga cgacgccgct catgccgaga gcctccgggc
    18961 cgcgggcgcg gcgagcagcc cttcgcagac ggccgcgatg ttcgcggcgg acgtccgtcc
    19021 gttctggagg gggacgagct tcttcagctc cccgatgtcg aagttggaat ggcattcctt
    19081 gccgagggcg aggccgacga acacacagga cgagaactgc gtgatcagaa cctcgcagtt
    19141 cgcgatcatg tgattcacgt ttccggacgt gaagacgagc gcgtcggggg cccatgcgcg
    19201 gatctcgcgg gtcgagcgct cgtggttctc gttcggatgg agcttgaaga cgagccgacg
    19261 cccgttggcg atcgtcagcg cccgatcgac gagcgcccgg cgatcgtccc gcttgaacgt
    19321 ctcgcgggtg tcggacgtcg cgacgaggac gtggccgcga tacgggaatt cgttctcgag
    19381 ataggacgcg cagtcgtcga agttcggaat ccccgtgacc tcgatccacg cgcgcggaac
    19441 gcccttcgcc gcgaagaact cccgatagcc ctcggacgcc acacagaact tgtcgtactt
    19501 cagcgagagc cccgtcgtgg aggtcgatgc gacccagagg ggcaagttcg ccttccggac
    19561 gaggtggtac gcgaacgtct ccgggtccgt catcccttcc tgcacgagca cgagaggctg
    19621 attccggacg ttcttcggga ggacgaggtc cgagcacgtg acgacgaggt cgtaatccgc
    19681 ggcgacgccg ccctcgtcga cgggcagcgt gtggctgcgg aggtactcct ccgtcatccg
    19741 gcgcatcggg ccgccgaaga tcgtgaagtc cagaaggccc gccttctgga gcgaccgcag
    19801 cggctggtcg cagtagaacg gcgagtacgc gcagtcgcac gtcgccgcga gccgctcgcc
    19861 gatcttgtgc atcatggtcg tctggttcag ggacccgccg atgagcagga cccgctttcg
    19921 cgccggtgcc gtctgcctca tgggtggtga atatgcgaac gggaatcgcg agggcacgtg
    19981 aagcgcacgc gaacgccgtg tgaaacgtgg gccccgcgtc tccggcccgt tcgagaattt
    20041 cgtgttaaat cgacgtgaga agccgtcgcg caaccgcgtt catcacccat actcgcgcgt
    20101 caccggaaaa ggagagcaga ggcatggctt tgaacgtcga gacctcgggt gtcgtgcgcg
    20161 tgatcacgct cgaagggaag ctggggaccg agatcgcgca ggacctgaaa accgagttcg
    20221 acgcctacct cgcgggatcg ccgggcccga cgcttctcga catgtcacag gtgcgctaca
    20281 tcagctccta cctggtcggc gtcctcgtcg acatgcgttc gcggctcgcg gcgcgcgggt
    20341 acgcgctgca tttcgcgggg atcgacgcgc ggcaccggct ggtcctgacc atctccggcc
    20401 tcgacgagct gttcgggtac cacgcggagc gcgcggaggg aatcgcggcg cttgccgcgt
    20461 cggcgaaacc cgcctgagcc gcggacacgg atcagagtcg gatcttcatc gggcctgaac
    20521 gtcacacccg tacggtccct ctcgtgccga acccacgcgc cctccgccgg aagatccagg
    20581 aactccggga agacgtctcc gccgaggggg acgcgctggc cgcccgctgg aaaccctggg
    20641 tccggcgccg cgagttcgcg ttcagcgcgc tcaacctcgc gcactatctc gcgctgcgca
    20701 ggcgggacct gaggaggctg caggaagctc tgatgccgtg ggggctttct tccctgggcc
    20761 ggtgcgagtc gcgcgtcctt ccgacgctgg acggcgtcct cgcgagtctc gacgcgctgt
    20821 gccgcgagca gacgccgggc gtcgagcacc cggatccgcg cgcctatttc cggggcctgc
    20881 gtttcctcga gcgccgcgcc cgccgggcgc tcggccccgc tccgcccgag caccacgtgc
    20941 gcgtcatggt gaccctgccg ggcgaggcgg cgacggagcc ggggatcgca aaagcgctgc
    21001 tggagcgcgg agcttcctgc ttccggatca actgcgcgca cgatggcgcg gaggcgtggg
    21061 aggcgatggg ccggcacgtg cgccgcgccg agcaggaaat cggccggccc gctcgcatcc
    21121 tcatggacct cgcgggccca aagctgcgaa tcgccgcggt gagcgagcgg gcgcgcctcg
    21181 aacgaggcct gcggttcctt ctcacccgcg aagagccggt gcccggcgcc tcccctgtcg
    21241 aggcgagctg cgccgagccc ggcgtcgtcg aaacgctgcg agtcggcgag cgcgtcttcg
    21301 tcgacgacgg cagcgcggtc gcgacggtcg tctcgctgga gccgcgcggc gcgctcctgg
    21361 aagtcacccg gagcaaggga aagaagctca aaccggagaa ggggctcaac ttccccgact
    21421 cggacctccg ggtgccgtct ctcggctcga aggacctggc ggatctcgat tcgatcctgc
    21481 ggatcgctga cctcgtcggg tactcgttcg tccggtcggc cgacgacgtc gccctccttc
    21541 aggacgagat gcgggcccgg cttcccgacg cgtggcggtc gaagggcatc attgcgaaga
    21601 tcgagacgcc gctcgccgtc cggaacctcc cggacatcat cgttcgcggc gcaggttccc
    21661 agccgttcgg cgtcatgatc gcgcgcggcg acctcgcggt ggagctcggg ttcgtccgca
    21721 tcgccgagat ccaggaggag ctcctgtggt tatgcgaggc ggcgcacgtc cccgtcatct
    21781 gggcgacgca agtgctcgag gccttcgtga agaacggaat cccgacgcgg gccgagatga
    21841 cggacgcagc gatggccacg cgcgccgagt gcgtcatgct caacaagggc ccgtttgtcg
    21901 ccgacgcggt gcacgtcctt ggccgcgtgc tctcgcggat ggaggcccac cagttcaaga
    21961 aaaccccgca gctacgggcg ttgcaggcct ggcgctgacg ccggggcgac ctgaaccaca
    22021 gcttctgcgg tttgaaagaa atgtaaagaa aagcttgggc accttgccac ggacgaggat
    22081 cctgcccaga ttgtctttag gagtcgtctg tgcgggctcc atgctggtgt tgcgggtcgc
    22141 aaacatcgta cttcgcaggt gcctgcagta cctgcggcca ccgcgtctgg gtggatcgcg
    22201 cggttgacct cctgatcgcg gcaggagcga tcgtctctta cctgattctg cggaagctcg
    22261 agacctccat cccttgaatc agggaggctt ccacgcgccg ccagccgcgt cagctcgccg
    22321 ccgcgccttc gaccttgttc caggccgtgg cgatcgccgc ccgctccgcg ccgtacttcg
    22381 gctcgagccg gttcaaggag tcgaggaggg acgtcacgaa cttcgggacg ccgtctagaa
    22441 ggtacggatt cttcgtcgga tcgggcacga gtcgccggac gatgctgcgc gcgtcgcggc
    22501 tcacgtcggg ctcgttggcg ggaacgagcg aagcgaccgc gatcccgagt tccttcaggg
    22561 gcgcctccag ggccggttcc aaaggagaca gccgtgtgag tgccttcgtt ttcatggcac
    22621 gggtctatga cggtcaggtg aaatcggtgt gaagttcgac gcgccgcggc gcggcctgcc
    22681 gctgcacgcg gactgaagac gcctgtgatc ggatggaccc atgaagaaga cgccgaagac
    22741 catcggcctt gtcgcgcacg acaaccgcaa gcgcgacctc atcgagtggg tgaactggaa
    22801 cttcacgatc ctcgtccgcc accgcctcgt ctgcacgggg acgaccggaa ggctcgtcga
    22861 ggaagcgctg caggcgcagc tcacggccgc gaagaacccg gcgcccatgc cggaaatcac
    22921 gaagctgaag tcagggccgc tcggcggcga ccagcagctc ggcgcgatga ttgccgaggg
    22981 ccgggtcgac gtcatcgtct tcttctggga cccgatggag ccgcacccgc acgacgtcga
    23041 cgtgaaggcg ttgctccgga tcgccgtcgt ctacaacatt ccgatcgcct gcaaccgatc
    23101 cacggcggac ttcatcattt cgtctcccct gctcgcgcag ccctattcgc cgatcgtcaa
    23161 ggactactcg gcgcacgtgg accggaccgt gcagtcggag tgaggaggag ctccgccacg
    23221 cggtcgagga tccggcccgc cacgaccagc ttcgacgacc tttcgacgcg ctgaggctcg
    23281 gtgcccggcg cgagaaggac gacctcgttc tcgtcggcgt cgaagccgat tcccggccgc
    23341 gaaacgtcgt tcgcgacgag gaggtccacg cccttttcgg cgagcttcct cgccgcgttc
    23401 gcgaggaggt cctgcgtctc ggcggcgaac ccgacgagaa cggtcccctt gcgggcagcg
    23461 ccggatgcct ctcgcaggac gtcgggtgtc ggcgcgagct cgaggttcgg gatgcccgcg
    23521 gttcgcttga tcttctcccc cgcaggaacg gcgaccatga agtccgccgg ggcggcggcc
    23581 atgaggagca cgtcggcctc cgcgatctcg gcgagcacgg cctcgcgcat ctccgacgtc
    23641 cgttcgaccc ggacgagacg cgcgcccggc ggcggcgtga gcgacacggg gccgctcacg
    23701 aggacgacgt ccgcgccgcg ccgcgcggcc tcgcgcgcga gcgcgtaccc catcttcccg
    23761 gagctgcggt tcgagaggaa ccggaccggg tccaccggct cgcgggtcgg tccggccgtc
    23821 acgaggacac ggcgcccttc caacgagcgc gagaccaccc ccgcgtccag gatcgcgcga
    23881 gcgatgcggg ccgggggtgc gagccggccc gggccgacgt cgccgcaagc gaggtccccg
    23941 gcatcgggct cgacgaccgt cgcccctcgc gacccgagag tcccgaggtt ctcctcggtc
    24001 gcggggtggg tccacatcca cgtgttcatc gcgggggcga gcacgagcgc cttgcggtgc
    24061 ccgagggcgt acgtcgtgag ggcgtcgtcg ccgatcccgt gcgcgagctt cgcgaggacg
    24121 tcggccgtgg cgggcgcgat cgcgaggacg tcggcccagc gcgcgagcgc gatgtggtcc
    24181 accgctccgg aagacgcgtc ccagaggtcc gcgacgaccg gattcttcga aaggaccgcg
    24241 agcgtgaggg gggtgatgaa gcgcgtggcc gcgggtgtca tgaggacgcg gacctcggcg
    24301 ccggcttcca cgagggcgcg cacgatctcg gcggatttgt acgccgagat tcccccgctg
    24361 acgcccagaa cgatgtgaat ggtcactccg ccaaacggcg ccgattttcg cacggcgcgc
    24421 gctagaatcc cgattgcggc ccgaccggct tcggcgggca cgcggttcaa gggtctggaa
    24481 tcccgggtac gcatctcact tccccggaag gagacagcga tgagcacggt ccccgcctcc
    24541 gcggcgcgcg tcacgccccg ccacatcctc gcgaaaaagg cacgcggcga gcccgtcgtc
    24601 atggtgacgg cgtacgacca cccgtcggcg agtgcggcgg acgccgccgg cgtcgacgcg
    24661 atcctcgtcg gcgactcgct cggaatggtc gtgctcggtt acgacgacac gctgcgcgtc
    24721 acgatggacg acatgctgca ccacgcgcgc gccgtcgcgc gcgcgcggac ggccgccctc
    24781 cgcgtcggcg acatgccgtt cctctcgtac caggccgacg tcgccgaggc ggtgcgcaac
    24841 gcgggccgct tcctctccga cgggggcatg gacgccgtga agctcgaggg cggacgcgag
    24901 cgcgcgggga cggtccgcgc gatcgtcgaa gccggcatcc ccgtcatggg ccatctcggc
    24961 ctcacgcccc aatccatccg ggcgctcggc ggttaccggg tccagggcgc cacggccgag
    25021 gccgcgcggc gcgtcctcga cgacgccctc cggctggagg acgcgggctg cttcgcgatc
    25081 gtcctcgagg cgattccgga ccgggtcgcc gcgctcgtca cgagccggct cgccgtttcg
    25141 acgatcggga tcggcgcagg cgccgcctgc gacggccagg tcctcgtctg gcacgacctc
    25201 ctcggctggg aagagaggat cgcgccacgt ttcgtgaggc gatacgccgc gatggcggaa
    25261 gaggcgcgcg tggcgctcgg ccgctacgga gccgaggtgc gcgagcgccg cttcccggcg
    25321 gccgagcacg tctacccgat cgcggatgcc gagtgggcgc ggctccaggc cgacctcgcc
    25381 gcgcgtccgc cggccgcccg ccgccccgag tccgtcgcat ggtgatcgtc cggacgccct
    25441 tcgaagcccg cagggagcgg cgcgcgctcg gcggatcagt cgggttcgtg cccacgatgg
    25501 gagccctcca cgaggggcac ctctcgctcg tgcgccgcgc gcgggacgag aacgcgcacg
    25561 tcgccgccag cgtgttcgtc aacccggcgc agttcgggcc gcgggaagac ctcgcggcct
    25621 acccccgtgc actcgagcgg gatctcggcc tcctcgagcg ggagggcgtc gccctcgtcc
    25681 tcgcgccgga ggccggcgcg atgtacccgt ccggcttcga gacgtgggtc gcgccgggcc
    25741 gcctcgccga gcgcctcgaa ggcgaacggc ggcccggcca cttccgcggc gtcctcacgg
    25801 tcgtcgcgaa gctcttcaac gcgatcgagc ccgaccgcgc ctacttcggc cagaaggacg
    25861 cgcagcaggc gatcctcatc cgccggatgg cgcgggatct cgacttcgcg gcggacatcg
    25921 tcgtctgccc gatcgtccgc gagcgcgacg gcctcgccat gtccagccgg aacgtctacc
    25981 tctctcgcga ggagagacgg gcggcgagcg tgctcgcgcg ggcgctcgca gcggcctccg
    26041 tcgcctggga cgcgggcgaa cgccgtggcg acgttctccg ccgcgcggcg ctcacggtcc
    26101 tcgcgacgga gccgctcgcg aatcccgact acgtctcggc agcggatccg tccacgctcc
    26161 aggaaatgga cggtccagcc gaccaccttc tcatctccat ggccgtgcgc ttcggcgcga
    26221 cgcggctgct cgacaacgtg gtcctgcccc acggcacggc gctctgagcg gaggaacaat
    26281 gcttctcgcg atcgacatcg gcaacaccac gatcaccctt ggcctctacg acggcgaaac
    26341 gccgggcccg cgctggcgcc tcgagacggt ccggacgcgt cttgcggacg agtacggcct
    26401 cgcgctttct tcgctcctcg cgacggccgg actgacgtcc gcggacgtcg gcaaagccgc
    26461 gatgtcctcc gtcgtgccgc cgctgaccgg gacgtggacc gaggtgtgcc gccggacgct
    26521 gcgcctcgaa ccgctcgtcg tcggggccgg cgtgaagaca ggcgtgcgcg tgctctacga
    26581 cgacccgaag gccgtgggcg ccgaccggat cgtgaacgcg gttgccgtca aggagatgtt
    26641 cggcgggccc gccatcgtcg tggacttcgg tacggggacg acgttcgatg cgctttccgc
    26701 caagggcgag tacctcggag gcgcaatcgc gcccggaatc ggcatcgccg ccgaagcgct
    26761 gcacgcgcgc gcggcgaagc tcccgcgggt ggatctcgtg cgcccgccgg cggcgatcgg
    26821 ccgcaacacc gagcacgcga tgcagtcggg cctcctgttc gggtacgtgg ggctcgtcga
    26881 ggggatggtc gcgcgcttcc gggccgagct cggcgggaag gcgaaggtcg tcgccacggg
    26941 gggctatgcc gagctcgtcg cgaaggagac gaaggtcctg acgacggtcg cgccgtggct
    27001 cacgctcgac ggcctgaggc tgatcgccgc gatgaaccgc tgatcggcaa gtcctacgcg
    27061 gccgcgaacg accgccatcc gaagtagagc ccggtcgcga ggaggaacgc acccgcgacg
    27121 gccgtagacc gcgcgatcca cgggccggag tggtccttca gtcggtgcgc ggccctgatg
    27181 aggagcgtga accaggaaaa gacgccgagc ccgacgccca gcgcgaaccc gccggctccg
    27241 gccggcgacg ggtccagaag ccggtgaccg atgagaaacg agatgaacag cgtccagttc
    27301 acgaacgggc ctggattcgc cgcgccctgc aggaacccgg tcagaaacgg caggcccgct
    27361 ccgacggcgc ccgcggccgc gccggatggc gtggccgaca cggatgcgcc ccgggcgtcc
    27421 acgagaagct tgactccgga ggcgacgagg aagagagcga ggggcccgcg aacccagggg
    27481 ctcgtgacga ccctctcgat cacccacccg actccgaacc cgatgagcgc gcacgtgagg
    27541 aggtccacgc tcgcgcctcc gagcgcgacg ccgtacgcct gacgcttctc gccgagcagg
    27601 ctccggcgca ggcacgtgat gccgatgggt cccgggggaa tggcgccgac gaagccaccg
    27661 acgaacccgc acacgaggcc aagcggaaac gccgcgagca ttcaggattc tctcgagagc
    27721 tgatgaagat ttggttcagg ccgcgtg
//
LOCUS       DAOMFY010000021        27046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_584_length_27046_cov_7.829832, whole genome shotgun sequence.
ACCESSION   DAOMFY010000021 DAOMFY010000000
VERSION     DAOMFY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_584_length_27046_cov_7.829832"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="PLB01_03435"
     CDS             complement(<1..1478)
                     /locus_tag="PLB01_03435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ76383.1"
                     /translation="MGLVYLAHDRRTGLPVAIKVMSRWSADPELHARFLKENRILSSL
                     NHRNIVRCYEITESREGTPSIVMEYVDGVDFRAFEGRPFPELLPLMIQALMGLAYLRS
                     QNILHRDLSSNNIFVTLENQTRVTKILDFGVAKILQQQAADGDVKTRTGQFLGKFAFA
                     SPEHFRPSAVDWRSDVYSLGVIFHRLLTNAPPITVARKSNYYDWMVAHQKDNVFEVLA
                     PPGLPDLPEPLRAVVRRMLARDPDDRPQSYEEIIHVLDRIQRGLPPALEPDPFDLRTL
                     PPPVEGRIGSSGSGKRLSIPSAPAVSPAAWSDAAAPDAESPSVEESQQKRWPELDARE
                     PQHTERFVPPPSRAVPGIPEEEEIPSEPPPPGDSRGWGSASEAGPARTELLPPPGPAD
                     AMKTATTVTTGRPERIPEVPVWSGGAATDAPAGAAGSSSSRLLEEKTERLDDVIEKLR
                     QQALLEPLPPVKLAPSPEPPPSPAPPPAPPPSRPVSRPEPVPT"
     gene            1651..2418
                     /locus_tag="PLB01_03440"
     CDS             1651..2418
                     /locus_tag="PLB01_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76384.1"
                     /translation="MRIARGAAAALLFLFLAAAPPAAGLGRKLSLDQIVQQGKSVLVG
                     TVVCASTRWGEGRKMIWTDYEVSVQETWKGSPERVVTLSFAGGTLDGRTIAVTHVPRL
                     SVGETYVLSLNDLARAYTSPVVGAEQGLFREATDQATGKRILLDADGWAVGLDREGGL
                     MPLAFAQAAESPGTVVLRREAPQLGTHARERAAPRGIPGATYSDGNGKPLPAPSGLPT
                     LAAAASSGPATGGQPLTRDALRTAVRHVLEPQQPADR"
     gene            2432..5356
                     /locus_tag="PLB01_03445"
     CDS             2432..5356
                     /locus_tag="PLB01_03445"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQQ76385.1"
                     /translation="MKRTGFLIGTIAAAALLLPDSSALAQANSGHPGNEGCWCGFQTG
                     IVRNYVVRGTSSTAAQKDAAADMIREWNRYVSLFNVSVDSSSSLGSQNGVNEVNVFIS
                     NEESKARYGIELDANLFGVAVMFPEAGFGAFNQCKDFRPDGCSAFTETDVVVNAGFPS
                     GWTGDWYAPGNDTQGGVALVQATVLHEVGHTLGLHHVFDVASGAGYGNSLSTMNYLND
                     DVGKFVTRMDSKTIRAEYPAAARSLVDVAIFPFAYGNAKYKQVYAALSKTSVLPGESF
                     TLSNWLIQNVGSQATGALRVTFYLFPAGSRAYPQPGDLAVGNVDFAAGADVDAEQVMN
                     GTPLVVPAGAAAGDYWLGAIATVNGQEDSTYQPGKPSNNRFVVGHKPVAVRVLQGSGG
                     TTPLSADFQSAPAPAQAGQTVSFQDLSRGNPTGWLWDFGDPASGSANSSTARNPDHVY
                     QQAGTYTVRLTVSASGTASNSATRQVAVTPKPGTGTSSSALLVPIVLDLPGRFSSELT
                     LTNSGNTTATLRLRYTAAPVFGGAGSGTVSRTLGPGQQTVIPDAIGYLRQNGLAIPTG
                     SNQGGSLRVEFEGLSNSAAGYAAARTTAPISGIGRAGLSYPGVDVTKAFTEPVAIFGL
                     RQNASDRSNLALVNASTTSPVTLTVYVVRGDGSASIVFNPITLQPGQWSQYDGILTQA
                     DPALTEGWILIEPSSGNVPYLAYGVFNDNGTNDGSFVPAIPDSQVDALLGVPVIVEVG
                     SRFASELMLTNLSDKPAQAYFEFVESLSTPGGASTGVFYVDLAPLEQVLIPNVVDELR
                     SAGASIGPRGGSYAGALTVLFSAANGLTPGLAGVRTSSPSSTGPGRYGLFYAGDPFTN
                     SARDAWIYGLQQNASTRTNLAVVNAAVTNTPITIRLEIYSGTTGALVSRQTLATLQPG
                     QWRQYDSVLAQFAPGLENGYARLTVVDGDDSFFAYSVLNDGPAPNTGTSDGSYVPMVV
                     SR"
     gene            5476..5697
                     /locus_tag="PLB01_03450"
     CDS             5476..5697
                     /locus_tag="PLB01_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76386.1"
                     /translation="MAKAKAAKRKAPKGKKAAARKAAPKKRAPAKKKAAAGKPTRYCL
                     GRCATCGSPCCYNASHGGSHYCIDHAPNA"
     gene            5856..6308
                     /locus_tag="PLB01_03455"
     CDS             5856..6308
                     /locus_tag="PLB01_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ76387.1"
                     /translation="MKPAPPGWPRISSSLYYDDAAKAIDWLCEAFGFEVRLKVEGEGG
                     RIEHSELVFGDGVVMVAGAADKVGKFPFMRSPGQVGGANTQNMFAYVDDVEAHCARAR
                     GAGATVVVEPKISDYGEDYWSDKSYECVDVGGHHWWFAERLRSPKPKG"
     gene            6305..6649
                     /locus_tag="PLB01_03460"
     CDS             6305..6649
                     /locus_tag="PLB01_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ76388.1"
                     /translation="MSGIDQTLSALADPARRGAVDLLRVRPRRAGELAASLGLSAPAM
                     SRHLRVLRETGLVEEAHGAEPGDARVRVYRLKPGPFDALRAWVADVESFWTLELAAFK
                     RHAERAHQRKGQ"
     gene            6646..7182
                     /locus_tag="PLB01_03465"
     CDS             6646..7182
                     /locus_tag="PLB01_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQQ76389.1"
                     /translation="MSESPLPGDSARVSVFVAMPPEAAWDVFTRETDLWWRQGPRFRI
                     AGARRGVLGFEPGPGGRLFETWGEPPDARTHKVGRILAWEPPSLLEMEWRGVNFKPHE
                     STRVTVRFAPQRDGTLVTVQHSGWSALPADHPARHGLEGAAFARMIGLWWGDLLTSLR
                     EHTAEKEAGPGSGPPRPL"
     gene            7192..8256
                     /locus_tag="PLB01_03470"
     CDS             7192..8256
                     /locus_tag="PLB01_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQQ76390.1"
                     /translation="MSASLHRSGETPRTARFRQPAEWDRHEAVWLAWPSHEDLWEDAL
                     PGVRAAFTEFAAAIADRDVAGNPRGERLNVLVPDDENGRLAATALAGLGARYFVIPFG
                     DIWLRDTAPVFLVGPGHQRAAASFRFNGWGGKYVLPHDDAVSERVAMASGLPAYRFPW
                     ILEGGSVEVDGEGTCLTTRQCLLNRNRNSGMSPKAIEDGLKDALGVSTVLWLGDGLLN
                     DHTDGHVDTIARFTAPGEVVCMAPSGDDDPNHRVLLQIARDLGKMRDAEGRPLHVTTV
                     PSPGLVSGPDGEVMPASYVNFYIANTTVVVPAYGVPNDEAARKSIGALFPGRRAVSIP
                     ARELLEGGGAFHCISQQVPE"
     gene            8253..9104
                     /gene="aguB"
                     /locus_tag="PLB01_03475"
     CDS             8253..9104
                     /gene="aguB"
                     /locus_tag="PLB01_03475"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HQQ76391.1"
                     /translation="MTEVQNVTFAAVQCALGGSVDENVVKVEALVREAARRGATVILP
                     PELFEGPYFCREERDRFFAWARPAEGHPTIARFSALAKELGAAIPVSFFEKAGHAYYN
                     SLAMVDADGTVLGVYRKSHIPDGPGYEEKFYFRPGDTGFRAWNAKMAHLGVGICWDQW
                     FPEAARSMLLLGADVLLYPTAIGSEPENPALDTRDLWQRAMLGHAVSNVAPVIAANRV
                     GNEGGQVFYGSSFVANQRGEMLAELSREEEGVAVATIDLAQVRRDRASWGFFRDRRPD
                     LYGRLAT"
     gene            9208..12279
                     /gene="fdnG"
                     /locus_tag="PLB01_03480"
     CDS             9208..12279
                     /gene="fdnG"
                     /locus_tag="PLB01_03480"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:9781..9783,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HQQ76392.1"
                     /translation="MGLTRRTFLKAGTAGGAVALGFDLHEARAEMREFKISRTTETRS
                     TCPYCSVSCGVIIHTLGDRAKNASASVVHVEGDPDHPINRGTLCPKGITLKEDIQHAH
                     RLTKPMVRRPGSDKWEDISWDEAIAGIAKHVKTTRDKCFVTKNAKDQVVNRNPGMIVI
                     GGCTDTNEFALLQAKANASWGVVYRDTQARVUHGPTVASLAATFGRGAMTNGWVDIKN
                     ADVVFVMGGNPAENHPCGFKWAIEAKKTRNAKIVAVDPRFHRTAAVADLYSPIRAGTD
                     IAYLNGIIRYALEKNRFHEDYVKLHTNGPYVIGEKFGFNEGLFTGFDGKAYDKTQWAY
                     EADPKTKGYVVDPTLQNPRCVFQLLKKHVDRYTPEMVERICGVPKETFLKVAEMVTST
                     GNAQKVGTILYALGWTQHSTGVQIIRTAAMLQLLLGNVGRPGGGVNALRGHSNIQGAT
                     DMGGTFDILPGYLKTPAAPLQTLKEYLDTNTPTPLNKVAWASMNYWQNYPKFMVSYLK
                     AVWGAKATKENDFGYAWLPKVDGNYSWMYMFDDMYRGSSKRAGGTEPGPEGLITFGMN
                     PVATGPNSPKMVAALSKLKWLVVVENVETETASFWKAPKEYGGVDASKIATEVYLLPA
                     ANFAEKDGTFTNSGRWAQWKWKAIDPPGKAKSDQEILARLVLAVKDLYKKEGGALPEQ
                     VLNVSWAYTNPVSPDLGEVLKEVNGKAITDIPDPKDKTKILKTAGQQLDGFGQLQDDG
                     STLCGNWLYGGAYTEAGNMTQRRNNADPLGLGMFHQWAFSWPANRRVMYNRASADKDG
                     VPWDATRAGIKWNGEKWVGDVPDIKPDSKPGEFGAFIMNAEGVGRLFSAGLNDGPFPE
                     HYEAVEAPIENPLHPKVSANPVALKFSSDKDPFATPDKFPVVCTTYRLTEHFHFWTQH
                     NSRLNMVQPDFFFEIPEEFAKELGVANRGNIKVSSPRGAITAMAMVTKRLKPMKVDGK
                     NVWQIGMPIHWGFAGNPGHTGPLANFLTPSAADPNTWTPEFKAFLVKVEKA"
     gene            12290..13177
                     /gene="fdxH"
                     /locus_tag="PLB01_03485"
     CDS             12290..13177
                     /gene="fdxH"
                     /locus_tag="PLB01_03485"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HQQ76393.1"
                     /translation="MSVQNTLEVVRSSAQVWGSGSGLRSLPTVSKLVDTSTCIGCKAC
                     EVACQEWNDLKSVATVNDGDYQTLKTLDAEFWNLIRFDEHEVDGGIKWLMRKDQCMHC
                     AEPGCLEACPAPGALVQYANGIVDVNPAQCIGCGYCETGCPFDVPHFSAKTGKMGKCT
                     LCVDRVSVGLEPACIKSCPTGCLHFGTKEQMVALGNQRVAALKAAGYADAALYDPMGV
                     GGTGVVTVLAFGSHPEWYGNLPKAPRIPMAVTFWKKWLRPIGFLAIFGAIVGAFGHHL
                     YHGNKGHADPGPVGPDEKG"
     gene            13189..13878
                     /locus_tag="PLB01_03490"
     CDS             13189..13878
                     /locus_tag="PLB01_03490"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HQQ76394.1"
                     /translation="MSKVVRQIGFDEGAYVAREKEDVVVGNEIIRHRLSSRIIHWAVA
                     ATFLANIITGLPIWSPVFGWMATLVGGLSVCRIVHPWFGLAFALASLAMAVHWWSEME
                     MTKEERGWVGPKLLEYLRDATNHDKIGKYNGGQKVYFFTSKLGALGLLVSGIVMWYPT
                     SFPAWTRQIAYLLHNVSFLLFTVSLVFHVYLATVAEPGTFNSMTRGTVSKAWARIHHP
                     KWYRELMGLEK"
     gene            13883..14674
                     /locus_tag="PLB01_03495"
     CDS             13883..14674
                     /locus_tag="PLB01_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ76395.1"
                     /translation="MERKSALVTGASGGIGAAIATSLARTGAHVLLVARREEELEKVA
                     SAIRAAGGTASVHPADLTDDAAVEHLARRVTGEVGAPDILVNNAGAGQWKFIEETSAE
                     EARQMVALPYVAAFSVTRAFIPEMLRRGSGHVVNISSAASRVAWPGATAYVAARWAMR
                     GFTEALRADLRGTGIGVTLFESGVVETPYWQHNPGSRERVPGIGTFLLRTLTPEEVGE
                     AVVNGIRRKRRLVVLPFMLRVIYALHAVCPWLVQGLVVVTGHKRR"
     gene            complement(14684..15397)
                     /locus_tag="PLB01_03500"
     CDS             complement(14684..15397)
                     /locus_tag="PLB01_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76396.1"
                     /translation="MLLTLVVAGPVLAEGFRLDIEGGPVWQTRNDFAVPGTTGTRVAL
                     ADYDDGPFASGRATLTWDFSPRWSARFVAAPLAASVDFTPTAPVVFAGTTFPAGEPLN
                     AGYRFNSWRLSGYYRFPSSSPLSFRLGLTAKIRDARIDISGAKGSAEKSDTGFVPLIY
                     AGARWQLAERFALDLESDALGAPQGRAIDVSLKGDWSVSRSVSLYAGGRVLDGGADND
                     EVFSFATFWYGFVGVGIRF"
     gene            complement(15555..17483)
                     /locus_tag="PLB01_03505"
     CDS             complement(15555..17483)
                     /locus_tag="PLB01_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQQ76397.1"
                     /translation="MTRITSRGAVGAALVAFGLSAPALAQDAGEHDPAALAKAFPKAS
                     YSPYAGRDYPTRPYFGDTHLHTGFSMDAGAFGARLTPRDAYRFARGEEVTSNSGQPVK
                     LSRPLDFLVVADHSDGMGFFPQLMSGDPGLLATPQGRKWYDQIKGGQGADAALDIIVS
                     FGQGKLPKGFPLPGTPAYRNAWMETIKAAEAYNDPGRFTAFIGFEWTSNTGGNNLHRN
                     VIFRGSGAQAGLVEPYTTLRPLGSDNPEDLWKWMTMAEQKTGSDVLAIAHNGNLSNGI
                     MFPMVEAFGKKLDRTYVETRAKREPLYEMTQTKGTGEAHPFLSPNDEFAAFELWDKGN
                     LDGSVAKKNEMLEYEYARSAYKNGLKLEQQLGTNPYKFGMIGSSDAHTGLPAMEEDNF
                     FGKTAPQEPSPERLTKTFVANKKSGVTIMDWEVGASGWAAVWAKENTREAIFDAMQRR
                     ETYATTGTRMVVRFFGGFDFEPNDAYDRMPGRVGYAKGVPMGGDLSKAPAGKAPTFLV
                     GALRDPIGANLDRIQIVKGWMDAKGELHEKVYDVAWSGSRKPDPKTGKLPPVGDTVDV
                     AKATYTNTIGAPELVAVWKDPAFDPAQRAFYYGRVIEIPTPRWTAYDAVRFGTKPLPG
                     TRMTITERAYTSPIWYTP"
     gene            17748..19238
                     /gene="pap"
                     /locus_tag="PLB01_03510"
     CDS             17748..19238
                     /gene="pap"
                     /locus_tag="PLB01_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HQQ76398.1"
                     /translation="MFDTAELGHRIAKTVFDRAVPKLRADLLDAQYRLKEDGTFPVIV
                     LISGVRGAGKGETVNLLNEWMDPRHILTRAFDTPSDEERDRPPMWRYWRALPPKGKIG
                     ILFGSWYTAPIIDRVFRKTGRGDLLRSIEDINRFEKMLADEGALIVKFWFHLSKDAQR
                     KRLKALKADPGTRWRVTRRDRDFFREYDRFYRVSEETLRATSTADAPWIVVEGADAPY
                     RSLTVGRALLEAMDSRLEAPAGRGRPVPRAAPIVRPIDARDALTELDLSRTVTPKAYK
                     AGLSDWQGRLGRLARDAKFRRISVALALEGMDAAGKGSTIRRVTGALDARLYNVIPVA
                     APTDEERAQPYLWRFWRHLPRKGRVAIYDRTWYGRVLVERIEGFCSEADWMRAYAEIN
                     DFEEQLERAGTVVCKFWLQISREEQLRRFKERERMRFKRFKITPEDWRNRRKWSLYQS
                     AAADMIERTSTRSAPWTLVEANDKKFARLKVLETVCRRIELAFDGV"
     gene            complement(19249..21522)
                     /locus_tag="PLB01_03515"
     CDS             complement(19249..21522)
                     /locus_tag="PLB01_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ76399.1"
                     /translation="MTDALARWRLAAVVAAALVVASCPLALVRDSFRRPAPKSTAPAE
                     AAFVGRKVCARCHEKATKAWTGSHHDLAMTEATDATVRGDFSGAVFEGDGMKARFFRD
                     GKKFLIETAGPDGKPAVYEAAYTFGWDPLQQVLVRFPGGRMQAFSLAWDVPGKRWFFL
                     YPGRNIPPSDWLHWTRNGQNWNGMCAGCHSTNLVKGFDPKTNTFTTTWSEVDVSCEAC
                     HGPGSRHVAWADVPPMGRARVDNFGLVLKTSNVTNRELVELCAPCHSRRVELGGYDHR
                     RPELLDNHVPVLLAEGAYHPDGQILDEDFEYGSFVQSKMFRMGVRCTDCHDAHTLKLR
                     KDGNGVCLQCHSAPAYDDARHHFHKKEWKGKPSDGAVCASCHMPKSPFMVVHMRADHS
                     IRVPRPDLTRDLGVPNACSRSGCHADEPLGWVTAAFDKWYGTARKPHYGTVLAAGRKG
                     SPAAKADLLRLEADVLSPTLVRATALSLLGGYPGDDVTAAFRRALLDEEPLIRRTAVA
                     VAPIADEAERVARLAPLLADPFRAVRLEAAAQLAGTSPALLKPYQKDALDTALADYVK
                     AMEYALDFSYAGHNLGLMYERMRDAVKAEAAYRAALAVDDLSYPPKANLAMLLARQGK
                     SAEAEKLLREVLGAYPDRSEVAYALGLLVAEKGAMPEAAALLARAAAGMPGNARAAYN
                     AGLALARVGRDAEAEKMLRQAAAIDPSSYDALFALADFLLRKGKPAEAGPLADRMAAI
                     DPARPEAAQIRGLLGRR"
     gene            complement(21527..22006)
                     /locus_tag="PLB01_03520"
     CDS             complement(21527..22006)
                     /locus_tag="PLB01_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQQ76400.1"
                     /translation="MERVTERRSFLKLAWLSLGIAALAEASWIVVAFLRPRPKAPKVA
                     GSLVVAGPSDGFAPGSVTAFPAGKFYLVRLADGGFLALHRQCTHLGCSVPWDEAAGRF
                     ACPCHASVFDLRGDVLAPPAPRPLDLFAVRIENGIVKVDVSAPIRRSSFDAAQVTRS"
     gene            complement(21994..23304)
                     /locus_tag="PLB01_03525"
     CDS             complement(21994..23304)
                     /locus_tag="PLB01_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQQ76401.1"
                     /translation="MKPAESARTVVSTFVLHLRPVRVPAATLRFTHTFGLGGSSLVLL
                     SILLATGSLLMFGYAPVPGDAYASIEKLQSEVTFGAFVRAVHHWSANLLVLVVVLHLL
                     RVVFTGGFLGTRALNWLVGLFLLAAIAASSFTGYLLPWDQLAYWAVTIVTGMAAYVPL
                     AGPALAAFLRGGEEIGPATLGTYFALHTQWLPVLLFAGAGFHFWRVRKAGGVVLPPDA
                     ERDENGKPAQVLFLPELLWRELAAALVVLASVTLFSALVRAPLGPAANPGLSLNPAKA
                     PWYFLGLQELLVHLHPLFAVVVVPLLGIAALCALPFLGFPDDEPRGAWFLSETGRRTA
                     KLAAAAALGATPLLVLADALLLGKPGAPSGGAAFVLRGVLPAAALLLGAAAGFRLLRS
                     RFGASRLEAAQALFVFFGTAFAVLTVVGIWFRGAGMKLVWPWNA"
     gene            complement(23389..23922)
                     /locus_tag="PLB01_03530"
     CDS             complement(23389..23922)
                     /locus_tag="PLB01_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQQ76402.1"
                     /translation="MKLSRFATLAFVVTAVACSTLEVNTDYAPGTDFSKYKTFTLKAG
                     TAPRNPIAAERFAQSLGNALAARGLRQVPEGGDLTVFTHFSLGKDVQLNSYGYGGWGG
                     GWRYGGMGGMQTTTVQEIPTGTVVIDLVDASTKTAVWRGMAKDQISTSATPEERQQKA
                     DQVAQKLFENYPPQAKK"
     gene            24038..24745
                     /locus_tag="PLB01_03535"
     CDS             24038..24745
                     /locus_tag="PLB01_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76403.1"
                     /translation="MNKRILASTLAVLLLSAALPLTAQMEPKGSLQGLQPPKADVPQL
                     MTLQGEFVRMAYNNEGYAVLGYRLVQDEVGKPWVLLNVGMTLQEGVKNYKLKREHLTL
                     KTPDGTVVPLATQQEYMADPYLRALNQRAKVVKDSINYFPAGPGRPCAMQFFADLDKP
                     GLAYNEVDMTDSRDCMGRLYFKVPGGIQVGQYWLQVQFATSMIQVPFRVFTKEQEKDF
                     RKQWEDLKKAHDETFSM"
     gene            24750..25550
                     /locus_tag="PLB01_03540"
     CDS             24750..25550
                     /locus_tag="PLB01_03540"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HQQ76404.1"
                     /translation="MAKSLDPAAAFVARAARAAGLVAAAPLVAEPLAFASGLFRLQGR
                     LAAAVNARHVDAAFTGAPADFPRVLDLLAPLLTYAAEKGPPELAEVARARHADADATA
                     LARLSVYWSGEREAREDYLSRAFLRPYAEVLAARGVTLERPRHEGHCPACGNGAVVSV
                     RREVPESMGAARSLVCALCGTEWPFARIRCAACHEEDPAKLPSFRTEAHPAVRIEACE
                     TCKRYVKSLDLTLDARPLPEVDDLVSLAMDLWAVEEGWTRLEPGWAGI"
     gene            25535..25924
                     /gene="crcB"
                     /locus_tag="PLB01_03545"
     CDS             25535..25924
                     /gene="crcB"
                     /locus_tag="PLB01_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HQQ76405.1"
                     /translation="MGGNLKPSLLVLAFVGGGIGSACRYAMTLALQQRLPGPFPIGTF
                     SVNLAGCFAIGLVGALGLERAALSPEARTFLMVGVLGGFTTFSSFAWETLELISARDV
                     LRAVVYACGSVVLGLFGVLLGRALGRL"
     gene            complement(25921..26223)
                     /locus_tag="PLB01_03550"
     CDS             complement(25921..26223)
                     /locus_tag="PLB01_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76406.1"
                     /translation="MSSDPDFDPLAGILVSSHRVERIAARARDVAHTFSAWALSVHTA
                     QGPGRIVRIELTPETTLWRGDGVFLGWTTERLVAAWDALRAAEPETESSPDFPQLG"
     gene            complement(26234..>27046)
                     /locus_tag="PLB01_03555"
     CDS             complement(26234..>27046)
                     /locus_tag="PLB01_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQQ76407.1"
                     /translation="LLVAPSAHAEDASTAPPPPPPVFSFEETGALAAGDATGVLLAPL
                     KWDGTDWLKFGGAAAAVVATGFLLDNTLQDATQRSRTSARDDAASVVACFGLGCSFAV
                     LGGFAVVGFVGKNREAMNVAVDGLLASAIATGVVAPVSKFVVGRARPNAGEGPDHFAP
                     FSGDASFPSGHATQAFAVASVIAAHDGRLWVSVVSYGLAGSVGLARVYQNEHWASDVL
                     AGAILGTVVGNAVVRVNDAIRAGRRGSGGRVSIVVTPLLLRKGGGAALHASF"
BASE COUNT         4338 a         9445 c         9302 g         3961 t
ORIGIN      
        1 gtaggcacag gctccgggcg gctgacgggc cgcgacggag gcggcgcggg aggcggcgca
       61 ggcgaaggag gaggctccgg agagggagcc agctttacgg gaggaagcgg ttccaggaga
      121 gcctgctgcc tcagcttctc gatcacgtcg tcgagcctct cggtcttctc ttccaggagc
      181 ctcgacgaag aggagccggc ggccccggcg ggcgcgtccg tggccgcgcc tcccgaccac
      241 acgggaacct cggggatcct ctccggcctc cccgtggtca cggtcgtcgc ggtcttcatg
      301 gcgtccgcgg gaccgggcgg aggaagcagt tccgtcctcg ccggacccgc ctcggaggcc
      361 gacccccagc ccctggagtc acccggcgga ggaggttcgg acgggatctc ttcctcctcg
      421 gggattccgg gcacggcgcg agagggcgga ggaacgaagc gctcggtgtg ttgcggctcg
      481 cgcgcatcga gctccggcca cctcttctgc tgcgattcct cgacggacgg gctctccgca
      541 tccggggccg cggcgtcgga ccacgcggcg ggcgacaccg ccggcgcaga gggaatcgac
      601 aaccgcttcc cgctgcccga cgagccgatt ctcccttcga ccggcggcgg aagggtgcgg
      661 aggtcgaacg gatcgggctc gagcgcgggc ggcagaccgc gctgaatgcg gtcgagcacg
      721 tggatgatct cctcgtaact ctgcgggcgg tcgtccgggt cccgggccag catccgccgg
      781 acgacggccc ggagcggctc cggcagatcc gggaggccgg gcggcgccag gacctcgaaa
      841 acgttgtcct tctggtgcgc caccatccag tcgtagtagt tcgacttgcg ggccacggtg
      901 atcggcggcg cgttcgtcag gagccggtgg aagatgacgc cgagcgagta cacgtcgctg
      961 cgccagtcga cggccgacgg gcggaaatgc tccggcgacg cgaacgcgaa cttcccgaga
     1021 aactgccccg tcctcgtctt cacgtctccg tccgccgcct gctgctggag gatcttcgcg
     1081 acaccgaagt cgaggatctt cgtgacgcgc gtctggttct cgagcgtgac gaagatgttg
     1141 ttcgacgaga gatcgcggtg caggatgttc tgactgcgga gataggcaag gcccattagg
     1201 gcctggatca tgagcggcag gagctcgggg aacggccggc cctcgaacgc gcggaagtcg
     1261 acgccgtcga cgtactccat gacgatcgag ggggtcccct cgcgggactc cgtgatctcg
     1321 tagcaccgca cgatgttccg gtggttcagc gaggagagga tccggttctc cttgagaaac
     1381 cgcgcgtgga gctccgggtc cgccgaccac cgggacatga ccttgatcgc gaccggaagg
     1441 ccggtcctcc ggtcgtgcgc gaggtagacc agccccattc ccccttcgcc gaggacgcgg
     1501 gagatcgcgt actggtcgaa ctccggcggc gcgttcgaag tgcttgcgat gggtactctc
     1561 cgagtaccct cagtctagca gtcgcgaggg gcgacgattc cgcaggccgg cgcgccgggg
     1621 ctagaattcc gcgtacccaa ggaggttcgt atgaggatcg cccgtggcgc cgccgccgcg
     1681 cttctgttcc ttttcctcgc cgcggcgccg cctgccgccg gcctcggccg gaagctctcg
     1741 ctcgaccaga tcgtccagca ggggaaatcc gtcctcgtgg gtacggtcgt ctgcgcgtcg
     1801 acccgctggg gcgaggggcg gaagatgatc tggaccgact acgaagtttc cgtccaggag
     1861 acctggaagg gcagtcccga gcgggtcgtc acactgagtt tcgcgggcgg cacgctcgac
     1921 ggccggacga tcgccgtgac ccacgtcccc cggctctcgg tcggcgagac ctacgtcctc
     1981 tcgctgaacg acctcgcgcg ggcctacacc tcgccggtcg tgggtgccga gcaggggctt
     2041 ttccgcgaag ccaccgatca ggcgaccggg aaacggatcc ttctcgacgc ggacgggtgg
     2101 gccgtcggcc tcgaccggga gggcgggctg atgcctctcg cgttcgccca ggccgcggag
     2161 tcccccggca cggtcgtcct gcgacgggaa gctccgcagc tcggaaccca cgcacgggaa
     2221 agggccgccc cgcgcggcat tccgggagcg acgtactcgg acggaaacgg aaaaccgctt
     2281 cccgccccct cgggacttcc gacgcttgcg gcggcggcct cgagcggccc cgccaccggc
     2341 gggcagccgc tcacacgcga tgcgctgcgc acggcggtcc gccatgtcct cgagccgcag
     2401 cagccggcgg atcgctgacg tgaggcgtga cgtgaaaaga accggatttc tcatcgggac
     2461 gatcgccgcc gccgcgctcc ttctgccgga ttcctccgcc ctcgcccagg ccaactccgg
     2521 ccacccgggc aacgaggggt gctggtgcgg cttccagacg ggcatcgtgc gcaactacgt
     2581 cgtccggggc acgtccagca cggcggccca aaaggacgcc gccgccgaca tgatccggga
     2641 gtggaaccgt tacgtgagcc tgttcaacgt gtcggtcgac tcctcctcga gcctcggcag
     2701 ccagaacggc gtgaacgagg tgaacgtctt catctcgaac gaggagtcga aggcccgcta
     2761 cggcatcgag ctcgacgcca atctcttcgg cgtggcggtc atgtttcccg aggcgggctt
     2821 cggcgcgttc aaccagtgca aggacttccg ccccgacggg tgcagcgcct tcacggaaac
     2881 cgacgtcgtc gtgaacgccg gtttcccttc gggctggacg ggcgactggt atgccccggg
     2941 aaacgacaca cagggagggg tcgcgctcgt gcaggcgacc gtcctccacg aggtggggca
     3001 caccctgggc cttcaccacg tgttcgacgt cgcctcgggc gccggatacg ggaacagcct
     3061 ctcgacgatg aactacctga acgacgacgt cgggaagttc gtcacgcgga tggactccaa
     3121 gacgatccgg gccgagtacc cggccgccgc gcgatcgctc gtggacgtcg cgatcttccc
     3181 gttcgcttac ggaaacgcca aatacaagca ggtttacgcc gccctctcga agacgtccgt
     3241 gctcccgggc gagagcttca cgctgagcaa ctggctgatc cagaacgtgg ggagccaggc
     3301 aaccggcgct ctccgggtca cgttctacct gttccccgcg ggatcccggg cctaccctca
     3361 gcccggggac ctcgcggtcg gcaacgtcga cttcgccgcc ggcgcggacg tcgacgccga
     3421 gcaggtcatg aacggcacgc ctctcgtcgt accggcgggc gccgccgcag gagactactg
     3481 gctcggcgcg atcgcgacgg tcaacgggca ggaggattcg acctatcagc cgggcaagcc
     3541 cagcaacaac cggttcgtcg tcggccacaa gccggtcgcc gttcgcgttc tccagggatc
     3601 gggcggcacg acaccgctgt cggcggattt ccagtccgcg ccggcgcccg cgcaggcggg
     3661 ccagaccgtt tcgttccagg acctctcgcg cggcaacccg accgggtggc tctgggattt
     3721 cggagacccc gcttccggca gcgccaattc gtccacggcg cgaaacccgg accacgtcta
     3781 ccagcaggcc ggcacgtaca ccgtccggct gaccgtctcg gcctcgggca ccgcctcgaa
     3841 ttcggcgacg cgacaggtcg ccgtcacccc gaagcccggc acgggaactt cttcgtcggc
     3901 acttctcgtg ccgatcgtcc tcgacctgcc cgggcgcttc tcgtccgagc tcaccttgac
     3961 gaacagcgga aacacgaccg cgacgctgcg cctgcggtac accgcggcgc cggtcttcgg
     4021 cggagcgggc agcggcaccg tgtccagaac gctcggcccc ggccagcaga cggtcatccc
     4081 ggacgcgatc ggatatctgc ggcagaacgg actcgcgatt ccgacgggct cgaaccaggg
     4141 agggtccctc cgggtcgagt tcgaggggct ttcgaactcc gccgcgggct acgccgcggc
     4201 acggacgacg gcgccgatct ccggcatcgg ccgcgccggg ctttcgtatc ccggcgtcga
     4261 cgtcaccaag gcgttcacgg agcccgtcgc aatcttcggg ctgcgccaga acgcctcgga
     4321 ccgcagcaac ctcgccctcg tcaacgcgag cacgacgagc cccgtcacgc tcaccgtcta
     4381 tgtggtccgg ggcgacggca gcgcgtcgat cgtgttcaac ccgatcaccc tccagccggg
     4441 ccagtggagc cagtacgacg ggatcctgac ccaggcggac ccggccctga ccgagggctg
     4501 gatcctgatc gaaccctcgt cgggcaacgt cccctacctc gcctacggcg tcttcaacga
     4561 caacgggacc aacgacggct cgttcgttcc cgcgatcccg gacagccagg tcgacgccct
     4621 tctcggcgtg cccgtgatcg tcgaggtcgg aagccggttc gccagcgagc tgatgctcac
     4681 gaacctctcc gacaagcccg cccaggcgta cttcgaattc gtcgagtcgc tttcgactcc
     4741 gggaggcgcc tcgaccggcg tcttctacgt cgacctcgcg ccgctggagc aggtcctcat
     4801 tccgaacgtc gtcgacgagc tccgcagcgc cggcgcgagc atcggacccc ggggcggctc
     4861 ctacgccggc gcgctcacgg tcctcttctc ggccgccaac ggcctgacac ccgggctcgc
     4921 cggcgtgaga acgtccagcc cgtcgagcac cggcccgggc cgctacggcc tcttctacgc
     4981 cggcgacccg ttcacgaact cggcccgcga cgcctggatc tacggactcc agcagaacgc
     5041 gtccacgcga acgaacctcg ccgtcgtcaa cgcggcggtc acgaacaccc cgatcacgat
     5101 ccgtctcgag atctacagcg ggacgaccgg ggccctcgtc tcgcgccaga cgctggcgac
     5161 gctccagccg gggcagtggc gccagtacga cagcgtcctc gcgcagttcg ctcccggcct
     5221 ggagaacgga tacgcccggc tcaccgtcgt cgacggcgac gactccttct tcgcctacag
     5281 cgtcctgaac gacggcccgg ctccgaacac cgggacgagc gacgggagct acgttccgat
     5341 ggtggtttcg cggtgaggcc cgtccgtccc ggccgggcgc cctcgccgcg gaccttgcgg
     5401 ccgccgcaac gcgggactcg gccccccgcc ggatctgaga tacgctgtcc gtagagttcc
     5461 gataaggaga gtcgcatggc gaaagcgaaa gccgcgaaga gaaaagctcc gaaggggaag
     5521 aaggcggccg cccgcaaggc cgccccgaag aagagggcgc cggcgaagaa gaaagccgcg
     5581 gcggggaagc cgacccgcta ctgcctcggc cgctgcgcaa cctgcggctc gccgtgctgc
     5641 tacaacgcca gccacggcgg ctcgcactac tgcatcgacc acgccccgaa cgcctgacct
     5701 ccagccccgt ccggcgctcg cccgccgttg cctgagatga cgaccggccg gaaaaggccg
     5761 cttgacgtgc gggccgcgtc cgttctatag tttggtattg cattaactat tctagaaact
     5821 gataagtacc attcttcccg gcgctcggag gccccatgaa acccgcaccc cccggctggc
     5881 cccgcatctc gtcctccctc tactacgacg acgccgcgaa ggcgatcgac tggctgtgcg
     5941 aagcgttcgg cttcgaggtc cgcctcaagg tcgaaggcga gggcgggcgc atcgagcact
     6001 cggagctcgt cttcggcgac ggcgtggtca tggtggccgg cgccgcggac aaggtcggca
     6061 agttcccgtt catgcggagc ccgggacagg tcggcggcgc gaacacgcag aacatgttcg
     6121 cgtacgtcga cgacgtcgag gcgcactgcg caagggcccg cggggccggc gcgacggtcg
     6181 tcgtcgagcc gaagatctcg gactacggcg aggactactg gtcggacaag agctacgagt
     6241 gcgtggacgt cggcggccac cactggtggt tcgccgagcg gctgcgcagc ccgaagccga
     6301 agggatgagc ggaatcgacc agactctctc cgccctcgcg gaccccgcgc gccgaggcgc
     6361 ggtggacctc ctgcgcgtca gaccccggcg cgcgggcgag ctggccgcgt cgctgggcct
     6421 gagcgcgccc gcgatgagcc ggcacctgcg cgtcctgagg gagaccggtc tcgtcgagga
     6481 ggcgcacggg gcggaacccg gggacgcccg cgtccgcgtc tatcgcctca agcccgggcc
     6541 gttcgacgcg ctgcgcgcat gggtggcgga cgtcgagagc ttctggactc tggagctcgc
     6601 cgcgttcaag cggcacgccg agcgcgcgca tcagaggaag gggcagtgag cgagtctccc
     6661 cttccggggg actcggcccg cgtttccgtc ttcgtcgcca tgcctcccga agccgcatgg
     6721 gacgtcttca cgcgcgagac ggatctctgg tggcggcagg ggccgcgctt ccgcattgcg
     6781 ggggcgcggc gcggcgtcct cggcttcgag ccggggccgg gcgggcgcct cttcgagacg
     6841 tggggcgagc cgcccgatgc gcggacgcac aaggtgggcc ggatactggc gtgggagccg
     6901 ccgtcgctcc tcgagatgga gtggcgcggc gtgaacttca agccgcacga gtccacgcgc
     6961 gtgacggtcc gcttcgctcc gcagcgggac ggcacgctcg tgacggtcca gcactccggc
     7021 tggagcgcgc tgcccgcgga tcacccggcc cgccacggcc tcgaaggcgc agccttcgcc
     7081 cggatgatcg gcctgtggtg gggcgacctc ctcacgtccc tgcgcgagca caccgcggag
     7141 aaggaggccg ggcccggctc cggcccgccc cgtcctttat agtccggcgc gatgtccgcc
     7201 tcgctccacc gctccggaga gacgccccgc acggcacgct tccggcagcc cgccgaatgg
     7261 gaccgccacg aggccgtgtg gctcgcgtgg ccgagccacg aggacctgtg ggaggacgca
     7321 ctcccgggcg tccgcgccgc gttcaccgag ttcgccgctg cgatcgcaga ccgcgacgtg
     7381 gccgggaatc cccgcggcga gcgcctgaac gtcctcgtgc ccgacgacga gaacggccgc
     7441 ctcgcggcga ccgcgctcgc aggcctcgga gcgcgctact tcgtgatccc gttcggcgac
     7501 atctggctgc gcgacacggc gccggtcttc ctcgtcggac ccggccacca gcgcgccgcg
     7561 gcctcgttcc ggttcaacgg ctggggcgga aagtacgtcc tcccgcacga cgacgccgtg
     7621 tcggaacgcg tcgcgatggc ttccggcctc ccggcgtacc gcttcccgtg gatcctcgag
     7681 ggtgggtccg tggaggtgga cggtgaaggc acgtgcctga cgacgcgcca gtgcctcctg
     7741 aaccgcaacc gcaattccgg gatgtccccg aaggcgatcg aggacggcct caaggacgcg
     7801 ctcggcgtct cgacggtcct gtggctcggc gacggcctcc tgaacgacca cacggacggg
     7861 cacgtcgaca cgatcgcgcg cttcacggct cccggcgagg tcgtgtgcat ggccccctcg
     7921 ggcgacgacg acccgaacca ccgcgtcctg ctccagatcg cgcgcgacct cgggaagatg
     7981 cgcgacgccg agggccggcc gctgcacgtg acgacggtcc cgtcgcccgg cctcgtgtcc
     8041 ggcccggacg gcgaggtcat gcccgcgagc tacgtgaact tctacatcgc gaacacgacg
     8101 gtcgtggtgc cggcgtacgg cgtcccgaac gacgaggccg cccgaaaatc gatcggcgcg
     8161 ctcttccccg ggcgccgcgc cgtgtccatc cccgcccgcg agctcctcga gggcggcggc
     8221 gccttccact gcatcagcca gcaggtgccc gaatgacgga agtccagaac gtcaccttcg
     8281 ccgccgtcca gtgcgcgctc ggcggctccg tcgacgagaa cgtcgtaaag gtcgaggctc
     8341 tcgtgcgcga ggccgcccgg aggggcgcca ccgtgatcct cccgcccgag ctcttcgagg
     8401 ggccgtactt ctgccgcgag gagcgcgacc gctttttcgc gtgggcgagg cccgccgaag
     8461 gccacccgac gatcgcgcgc ttctcggccc tcgcgaagga gctcggcgcg gcgatccccg
     8521 tctcgttctt cgaaaaggcc gggcacgcgt actacaacag cctcgcgatg gtggatgcgg
     8581 acgggaccgt gctcggggtc tatcgcaaga gccacatccc cgacggcccg ggctacgagg
     8641 agaagttcta cttccgcccg ggcgacacgg gcttccgcgc gtggaacgcg aagatggcgc
     8701 acctcggcgt cggcatctgc tgggaccagt ggtttcccga agccgcgcgc tcgatgctcc
     8761 tcctgggcgc ggacgtcctc ctctacccca ccgcgatcgg ctcggagccg gagaacccgg
     8821 cgctcgacac gcgcgacctc tggcagcgcg ccatgctcgg ccatgccgtg agcaacgtgg
     8881 cgccggtgat cgccgcaaac cgcgtcggga acgagggcgg acaggtcttc tacgggtcgt
     8941 cgttcgtcgc gaaccagcgc ggcgagatgc tggccgagct ctcccgcgag gaggaaggcg
     9001 tcgccgtggc gacgatcgac ctcgcgcagg tccgccgcga ccgggcgtcc tggggcttct
     9061 tccgggaccg gcggccggac ctctacggcc ggctcgctac ttgaattcgc ccgttcggtt
     9121 tcgggagtga gatccggtct tgacacgccc cccctccggg gcatctgcta gggcgcacaa
     9181 gcagacagat tcggaggagg gaccaacatg ggcctgactc gacgaacctt cctgaaggcg
     9241 ggcacggccg gcggagccgt cgcgctcggc ttcgatctcc atgaagctcg ggcggagatg
     9301 cgcgaattca agatctcgcg cacgacggag acgcgttcga cgtgcccgta ctgctcggtc
     9361 tcgtgcggcg tcatcatcca cacgctcggc gaccgcgcga agaacgcgtc ggcctcggtc
     9421 gtccacgtcg agggcgatcc ggaccacccg atcaaccgcg gcacgctctg cccgaagggg
     9481 atcacgctca aggaggacat ccagcacgcc caccggctca cgaagccgat ggtgcggcgg
     9541 ccggggtccg acaagtggga ggacatctcc tgggacgagg cgatcgcggg catcgcgaag
     9601 cacgtcaaga cgacgcgcga caagtgcttc gtcacgaaga acgcgaaaga ccaggtcgtg
     9661 aaccgcaacc cgggaatgat cgtgatcggc gggtgcacgg acacgaacga attcgccctt
     9721 cttcaggcca aggcgaacgc gtcgtggggc gtggtctacc gggacaccca ggcacgggta
     9781 tgacacggcc ccacggtggc cagtctggcc gccacgttcg ggcgcggggc gatgacgaac
     9841 ggatgggtgg acatcaagaa cgccgacgtc gtcttcgtca tgggcggaaa ccccgccgag
     9901 aaccacccct gcggtttcaa gtgggcgatc gaggccaaga agacgcgtaa cgcgaagatc
     9961 gtcgcggtcg atccgcgctt ccatcgcacg gcggccgtcg cggacctcta ctcccccatc
    10021 cgcgccggca cggacatcgc gtacctgaac ggcatcatcc ggtacgcgct cgagaagaac
    10081 cgattccacg aggactacgt caagctgcac acgaacggcc cgtacgtcat cggcgagaaa
    10141 ttcggcttca acgaaggtct cttcacgggt ttcgacggca aggcctacga caagacgcag
    10201 tgggcctacg aggcggatcc gaagacgaag gggtacgtcg tcgacccgac cctccagaac
    10261 ccgcgttgcg tcttccaact cctgaaaaag cacgtcgacc ggtacacgcc ggagatggtc
    10321 gagcggatct gcggcgtccc gaaggagacg ttcctcaagg tcgccgagat ggtcacgtcg
    10381 acggggaacg cgcaaaaggt cgggacgatc ctctacgccc tcggatggac gcagcactcc
    10441 accggcgtcc agatcatccg gaccgcggcc atgcttcaac tactcctcgg caacgtcggg
    10501 cgacccggcg gcggcgtgaa cgcgctgcgc ggccactcga acatccaggg cgcgacggac
    10561 atggggggca cgttcgacat cctgcccggc tacctcaaga cgccggcggc ccctctccag
    10621 acgctcaagg agtacctgga cacgaacacc ccgacgcctc tgaacaaggt cgcctgggcg
    10681 tccatgaact actggcagaa ctatccgaag ttcatggttt cgtacctgaa agcggtctgg
    10741 ggggcgaagg ccacgaagga aaacgacttc ggctacgcgt ggctcccaaa ggtggacggc
    10801 aactattcgt ggatgtacat gttcgacgac atgtaccgcg gctcatccaa gcgagccggc
    10861 ggcaccgagc ccgggcccga agggctgatc acgttcggga tgaacccggt cgccaccggt
    10921 ccgaactcgc cgaagatggt cgcagccctc tcgaagctca agtggctcgt ggtcgtcgag
    10981 aacgtcgaga ccgagacggc cagcttctgg aaggccccga aggaatacgg cggcgtcgac
    11041 gcgtcgaaaa tcgcgaccga ggtctacctc ctgccggccg cgaacttcgc cgagaaggac
    11101 ggcacgttca cgaactcggg gcgttgggcg cagtggaagt ggaaggccat cgaccccccg
    11161 ggcaaggcga agagcgatca ggagatcctc gcgcgcttgg tcctcgccgt gaaggacctc
    11221 tacaagaagg agggcggcgc tctccccgag caggttctca acgtctcgtg ggcctacacg
    11281 aaccccgtca gcccggatct cggcgaagtc ctgaaggaag tcaacggcaa ggcgatcacc
    11341 gacatcccgg acccgaagga caaaacgaag atcctcaaga ccgccggcca gcagctggac
    11401 gggttcggcc agctccagga cgacggctcg acgctctgcg gcaactggct gtacggcggc
    11461 gcctacaccg aggcgggcaa catgacccag cggcgcaaca acgccgaccc gctcggcctc
    11521 ggaatgttcc accagtgggc gttctcgtgg cccgcgaacc ggcgggtcat gtacaaccgg
    11581 gcgtccgccg acaaggacgg cgtgccgtgg gacgccaccc gtgccggaat caagtggaac
    11641 ggcgagaagt gggtcggcga cgtgccggac atcaagcccg acagcaagcc cggcgagttc
    11701 ggagcgttca tcatgaacgc cgagggcgtc ggtcgcctct tctcggcggg gctcaacgac
    11761 gggccgttcc ccgagcacta cgaggcggtg gaggcgccga tcgagaatcc gcttcacccg
    11821 aaggtgagcg cgaacccggt cgccttgaag ttctcctccg acaaggaccc gttcgccaca
    11881 cccgacaagt tccccgtcgt ctgcacgacg taccggctca cggagcactt ccacttctgg
    11941 acccagcaca actcgcgcct gaacatggtc cagccggact tcttcttcga gatccccgag
    12001 gaattcgcga aggagctggg cgtcgcgaac aggggaaaca tcaaggtctc ctcgccgcgc
    12061 ggcgcgatca cggcgatggc gatggtcacg aagcgcctga aaccgatgaa ggtggacggc
    12121 aagaacgtgt ggcagatcgg gatgccgatc cactggggct tcgcgggaaa cccgggccac
    12181 acgggccccc tcgcgaactt cctgacgccc tccgcggcag acccgaacac gtggactccc
    12241 gagttcaagg ccttcctcgt gaaggtcgag aaggcttgag gaggcggcga tgagcgttca
    12301 gaacaccctc gaggtcgtcc gctcctccgc ccaggtctgg ggctccggct caggcctgag
    12361 atccctcccg accgtctcga agctcgtcga cacgtccacg tgcatcgggt gcaaggcctg
    12421 cgaggtcgcc tgtcaggagt ggaacgacct caagtccgtc gcaaccgtca acgacggcga
    12481 ctaccagacc ttgaagaccc tcgacgccga gttctggaac ctgatccgct tcgacgagca
    12541 cgaggttgac ggcggcatca agtggctcat gcgcaaggat cagtgcatgc actgcgccga
    12601 gcccggctgc ctcgaggcct gtccggctcc gggcgccctc gtccagtacg cgaacggcat
    12661 cgtggacgtg aacccggccc agtgcatcgg ctgcggctac tgcgagacgg gctgcccgtt
    12721 cgacgtcccg cacttctcgg cgaagaccgg gaagatgggc aagtgcacgc tctgcgtgga
    12781 ccgcgtgtcc gtcggcctcg agccggcgtg catcaagtcc tgcccgacgg gctgcctcca
    12841 cttcggcacc aaggagcaga tggtcgcgct gggaaaccag cgcgtcgcgg ccctcaaggc
    12901 ggcgggatac gccgatgcgg cgctctacga cccgatgggc gtcggcggga cgggcgtcgt
    12961 gacggtcctc gccttcggca gccatcccga gtggtacggc aacctcccga aggccccgcg
    13021 catcccgatg gccgtcacgt tctggaagaa gtggcttcgc ccgatcggct tcctcgcgat
    13081 cttcggggcc atcgtgggcg cgttcggcca ccatctctac cacggcaaca agggccacgc
    13141 cgacccgggt cccgtgggac ccgacgagaa gggctgaggg aggcgcgcat gagcaaggtc
    13201 gtccgtcaga tcggcttcga cgaaggcgcc tacgtcgccc gcgagaagga agacgtcgtc
    13261 gtcggcaacg agatcatccg ccaccggctc tcctcgcgca tcatccactg ggccgtcgcc
    13321 gcgacgtttc tcgccaacat catcacgggg cttccgatct ggagccccgt gttcggctgg
    13381 atggcgacgc tcgtgggcgg cctgagcgtg tgccggatcg tccacccgtg gttcggcctc
    13441 gccttcgcgc tcgcgtcgct cgccatggcc gtccactggt ggagcgagat ggagatgacg
    13501 aaggaggagc gcggatgggt cggtcccaag ctcctcgagt accttcggga cgccacgaac
    13561 cacgacaaga tcgggaaata caacggcggg cagaaggtct acttcttcac gtcgaagctc
    13621 ggcgcgctcg gtctgctcgt gtcggggatc gtgatgtggt acccgacgtc gttcccggcc
    13681 tggacccggc agatcgcgta cctcctccac aacgtcagct tcctcctctt cacggtctcc
    13741 ctcgtcttcc acgtctacct cgcgaccgtc gccgagcccg gcacgttcaa ctcgatgacg
    13801 cgcggaacgg tctcgaaggc gtgggcgcgc atccaccatc cgaagtggta ccgcgagctc
    13861 atggggctcg agaagtagtc cgatcgaaag gaagagcgct ctcgtcaccg gtgcctcggg
    13921 cgggatcggg gccgcgatcg cgacgtcgct cgcgagaacc ggcgcgcacg ttcttctcgt
    13981 ggcacgccgt gaagaagagc tcgagaaggt ggcctccgca atccgggcgg cgggcgggac
    14041 ggcctccgtc catcccgccg acctgaccga cgacgcggcc gtggagcacc tggcccggcg
    14101 cgtcaccgga gaggtcggcg cgcccgacat cctcgtcaac aacgcgggcg ccgggcagtg
    14161 gaagttcatc gaggagacga gcgccgaaga ggcccgccag atggtggcgc tgccgtacgt
    14221 ggcggcgttc tccgtgacgc gcgccttcat cccggagatg ctgaggcgcg gcagcggcca
    14281 cgtcgtcaac atcagctcgg ccgcctcgcg cgtcgcctgg ccgggggcca cggcttacgt
    14341 cgcggcgcgc tgggccatgc gcggattcac ggaggccctg cgcgcggacc ttcgcggcac
    14401 gggcatcggg gtgacgctct tcgagagcgg cgtcgtggag acgccctact ggcagcacaa
    14461 tcccggaagc cgcgagcgcg tccccggcat cgggacgttc ctgctccgga cgctcacgcc
    14521 cgaggaggtc ggcgaggccg tcgtgaacgg catccgcagg aaaaggcgcc tcgtcgtcct
    14581 gccgttcatg ctcagggtca tctacgcgct gcacgccgtc tgcccgtggc tcgtgcaggg
    14641 actcgtcgtc gtcaccggcc acaagcgccg gtgacggcgc cggtcagaac cggatcccga
    14701 ctccgacgaa gccgtaccag aacgtggcga agctgaagac ttcgtcgttg tcggcgcctc
    14761 cgtccagaac ccggcccccg gcgtagaggc tcacggaacg ggacacgctc cagtcgccct
    14821 tcagcgaaac gtcgatcgcc cttccctgcg gggcgccgag ggcgtcggac tccaggtcga
    14881 gcgcgaaccg ctcggcgagc tgccatcggg cgcccgcgta aatgagaggc acgaaaccgg
    14941 tgtcggactt ctccgccgag cctttcgctc cggagatgtc gatccgcgcg tcgcggatct
    15001 tggccgtcag gccgagccgg aacgagagcg gcgacgaaga cggaaaccgg tagtacccgg
    15061 acaggcgcca ggagttaaag cggtagcccg cgttcagcgg ctcgcccgcg gggaacgtcg
    15121 tccccgcgaa cacgacgggg gccgtgggcg tgaagtcgac actcgccgcc aggggcgcgg
    15181 cgacgaaccg ggccgaccag cggggcgaaa agtcccacgt gagcgtggcg cgccccgaag
    15241 cgaacgggcc gtcgtcgtag tcggcgaggg cgacgcgggt gcccgtcgtc cccggaacgg
    15301 cgaaatcgtt gcgggtctgc cacacaggcc ccccctcgat gtcgagccgg aagccctcgg
    15361 cgagcacggg accggcgacg acgagggtca ggagcaccag agaggccagt cgaaaaggcg
    15421 cgagcttcac catgccgaca ctacgacggc cgtggcccgg aggattccac cgggttgcgc
    15481 cggggggcga cgggtcgagg gcgtaaagaa cgtcctcgtg cggatcggaa aagccgagaa
    15541 gtagagccga caggctacgg cgtgtaccag atcggcgacg tgtaggcccg ttccgtgatc
    15601 gtcatgcgcg tgccgggaag cggcttcgtg ccgaaccgca cggcgtcgta cgccgtccag
    15661 cgcggcgtcg ggatctcgat cacgcgtccg tagtagaagg cccgctgcgc ggggtcgaag
    15721 gccggatcct tccagaccgc cacgagctcg ggggcgccga tcgtgttcgt gtacgtcgcc
    15781 ttcgcgacgt ccaccgtgtc gccgaccggc gggagcttgc ccgtcttcgg gtcgggcttg
    15841 cgcgagcccg accacgcgac gtcgtagacc ttctcgtgga gctcgccttt ggcgtccatc
    15901 cagcccttga cgatctggat ccggtcgagg ttcgcgccga tcgggtcccg gagagcgccc
    15961 acgaggaacg tcggcgcctt gcccgcgggg gccttcgaga ggtcgccgcc catgggaacg
    16021 cccttggcgt agccgacgcg cccgggcatc cggtcgtacg catcgttcgg ttcgaaatcg
    16081 aagccgccga agaaccgcac gaccatgcgc gtcccggtcg tggcgtacgt ttcgcggcgc
    16141 tgcatcgcgt cgaagatcgc ctcgcgcgtg ttctccttcg cccacacggc cgcccagccc
    16201 gacgcaccca cctcccagtc catgatggtc acgccgctct tcttgtttgc cacgaatgtc
    16261 ttcgtcaggc gctcggggct gggctcctgc ggcgcggtct tgccgaagaa gttgtcctcc
    16321 tccatcgccg gaaggcccgt gtgcgcgtcc gaactcccga tcatcccgaa cttgtacggg
    16381 ttcgtgccga gctgctgctc gagcttcagc ccgttcttgt acgccgaacg ggcgtactcg
    16441 tactcgagca tttcgttctt cttggcgacg cttccgtcga ggttgccctt gtcccagagc
    16501 tcgaaggccg cgaactcgtc gttcggcgag aggaaaggat gggcctctcc ggtccccttc
    16561 gtctgcgtca tctcgtagag cggctcgcgc ttcgcgcgcg tttccacgta cgtccggtcg
    16621 agcttcttcc cgaaggcctc gaccatcggg aacatgatcc cgttcgacag gttgccgttg
    16681 tgggcgatgg cgaggacgtc gctccccgtc ttctgctccg ccatcgtcat ccacttccag
    16741 aggtcctcgg ggttgtcgct cccgaggggc ctcagtgtcg tatacggctc gacgaggccc
    16801 gcctgcgcgc cgctcccgcg gaagatgacg ttgcggtgga ggttgttgcc gccggtgttc
    16861 gaggtccact cgaagccgat aaacgccgtg aaccggcccg gatcgttgta ggcctcggcc
    16921 gccttgatcg tctccatcca cgcgttgcgg tacgcgggcg tgcccgggag cgggaaccct
    16981 ttcggcagct tgccctggcc gaagctcacg atgatgtcga gggccgcgtc ggcgccctgg
    17041 ccgcccttga tctggtcgta ccacttgcgg ccctggggcg tggcgaggag ccccggatcg
    17101 ccgctcatga gctgcgggaa gaaccccatg ccgtccgagt ggtccgccac gacgaggaag
    17161 tccagcggac gggagagctt gacgggctgg cccgagttcg acgtgacctc ctcgccccgg
    17221 gcgaatcggt acgcgtcgcg cggcgtgagc cgcgcgccga acgcgcccgc gtccatcgag
    17281 aagcccgtgt ggaggtgcgt gtcgccgaag tacgggcgcg tcgggtagtc gcggcccgcg
    17341 tacggcgagt acgaggcctt cgggaacgcc ttcgcaaggg ccgcggggtc gtgctcgccg
    17401 gcgtcctggg cgagcgcggg cgccgacagc ccgaaagcaa ccagggcggc accaacggcg
    17461 ccgcgggacg tgattcgcgt catacgaatt ctcctgtcgt caatcatctc gtgcgtcgtg
    17521 agttcctcaa gagactggtc gttctttgag ccacgcgcgg cccaggtagt ttagagtgcc
    17581 tcgtagggat cggctcagca gtccgggctg cgcagcagtg gatcaccttc gcgcaggcca
    17641 ttcaataagt tacggtccac tgccggtcgt cgcatctgag gcatctgcat cagcgccggc
    17701 cgcccgcgag gcgcgtacga cgcgttcacg ggacgagagg tgcgaccatg ttcgacaccg
    17761 cggaactcgg ccacaggatc gcgaagaccg tcttcgaccg cgcggtgccg aagctgcggg
    17821 ccgacctgct ggacgcccag taccgcctga aggaagacgg gacgtttccc gtgatcgtcc
    17881 tgatctccgg cgtgcgcggg gcgggcaagg gcgagaccgt caacctcctc aacgagtgga
    17941 tggacccgcg ccacatcctc acgcgcgcct tcgacacgcc ctcggacgag gagcgcgatc
    18001 gtccgccgat gtggcgttac tggcgggcgc tcccgccgaa gggaaagatc ggcatcctct
    18061 tcggctcctg gtacacggcg ccgatcatcg accgcgtctt ccggaagacc ggccgcggcg
    18121 acctccttcg gtcgatcgag gacatcaacc gcttcgagaa gatgctcgcc gacgagggcg
    18181 cgctcatcgt caagttctgg tttcacctct ccaaggacgc gcagcggaag cgcctgaagg
    18241 ccttgaaggc cgatcccggg acgcgctggc gcgtgacccg ccgcgaccgc gacttcttcc
    18301 gcgaatacga ccggttctac agggtctcgg aagagacgct acgcgcgacg agcaccgcag
    18361 acgccccgtg gatcgtcgtc gagggcgccg acgctccgta ccgttccctc acggtggggc
    18421 gggcgctcct cgaggcgatg gacagccgcc tcgaggcgcc ggccggacgc gggcgcccgg
    18481 tcccgagggc ggcgccgatc gtccgcccga tcgacgcgcg cgacgcgctc accgagctcg
    18541 acctgtcgcg gaccgtcacc ccgaaggctt acaaggccgg gctctcggac tggcaggggc
    18601 gcctcggccg cctcgcgcgg gacgcgaagt tccgccggat ctccgtcgcc ctcgcgctcg
    18661 aaggcatgga cgcggcgggc aagggcagca cgatccgccg cgtcacgggc gcactggatg
    18721 cgcgcctcta caacgtgatc ccggtggccg cgcccaccga cgaggagcgc gcccagcctt
    18781 acctgtggcg cttctggcgc cacctgcccc gcaagggccg ggtcgcgatc tacgaccgca
    18841 cgtggtacgg gcgcgtcctc gtcgagcgca tcgagggctt ctgctcggag gccgactgga
    18901 tgcgggccta cgcggagatc aacgacttcg aggagcagct cgagcgcgcc ggcaccgtgg
    18961 tgtgcaagtt ctggctgcag atctcccgcg aggagcagct tcggcgcttc aaggagcgcg
    19021 agcgcatgcg cttcaagcgc ttcaagatca ccccggagga ctggcgcaac cgcaggaagt
    19081 ggagcctcta ccagtcggcc gccgccgaca tgatcgagcg cacgtcgacg cgcagcgcgc
    19141 cctggacgct cgtcgaggcg aacgacaaga agttcgcgcg cctcaaggtg ctcgagaccg
    19201 tctgccggcg catcgagctc gcgttcgacg gggtctgacc gctccgcctc agcggcggcc
    19261 gagaagcccc cggatctgag ccgcctccgg gcgggcgggg tcgatcgccg ccatccggtc
    19321 cgccagcggg cccgcctcgg cgggctttcc cttccgcagg aggaagtccg cgagggcgaa
    19381 gagcgcgtcg tagctcgacg ggtcgatcgc cgcggcctgc cgcagcatct tctcggcctc
    19441 ggcgtcgcgc cccacccgcg cgagggcgag tcccgcgttg taggcggcgc gcgcgttccc
    19501 cggcattccc gccgcggcgc gggcgagcag cgccgccgcc tccggcatcg ctcccttctc
    19561 ggccacgagg aggccgagcg cgtatgcgac ctcggagcgg tccgggtacg cgccgaggac
    19621 ctcgcgcagg agcttctcgg cctccgcgct cttcccctga cgtgcgagca gcatcgcgag
    19681 gttcgccttc ggcggatacg aaaggtcgtc caccgccagg gccgcgcggt acgccgcctc
    19741 ggctttcacg gcgtcgcgca tccgctcgta catgagcccg aggttgtgcc ccgcgtacga
    19801 gaagtcgagc gcgtactcca tcgccttcac gtagtcggcg agcgccgtgt cgagtgcatc
    19861 cttctggtag ggcttcagga gagccgggga cgtcccggcg agctgcgcgg ccgcttcgag
    19921 ccgcaccgcg cggaacggat cggcgaggag cggcgcgagc cgggcgacgc gctcggcctc
    19981 gtcggcgatc ggggccaccg ccacggcggt cctccggatc agcggttcct cgtcgaggag
    20041 cgcgcgccgg aacgccgcgg tcacgtcgtc tcctgggtat ccgcccagga gtgaaagggc
    20101 cgtggcccgc acgagcgtcg gggagagaac gtcggcttcg agccggagca gatcggcttt
    20161 cgcggccggg ctccccttcc gccccgccgc gaggaccgtt ccgtagtgcg gcttccgtgc
    20221 ggtcccgtac cacttgtcga acgccgccgt cacccacccc aggggctcgt ccgcgtggca
    20281 gcccgagcgg ctgcacgcgt tcggcacgcc gaggtcccgc gtcaggtcgg gccgcgggac
    20341 gcggatgctg tggtccgcgc gcatgtggac caccatgaac ggcgacttcg gcatgtggca
    20401 ggacgcgcag accgccccgt cgctcggctt ccccttccac tccttcttgt gaaagtggtg
    20461 gcgggcatcg tcgtaggccg gagccgagtg gcactgcagg cagacgccgt tgccgtcttt
    20521 cctgagcttc agcgtgtggg cgtcgtggca gtccgtgcat cgcacgccca tccggaacat
    20581 cttgctctgg acgaacgacc cgtactcgaa gtcttcgtcg aggatctggc cgtccgggtg
    20641 gtacgcgccc tcggcgagaa ggaccgggac gtggttgtcg aggagctccg gacggcggtg
    20701 gtcgtaaccg cccagctcca cgcgacgcga gtggcacggc gcgcacagct cgacgagctc
    20761 gcggttcgtg acgttcgagg tcttgaggac gaggccgaag ttgtcgaccc gggcgcggcc
    20821 catcggcggg acgtcggccc aggccacgtg ccgcgagccg gggccgtggc aggcctcgca
    20881 gctcacgtcg acctcggacc acgtggtcgt gaacgtgttc gtcttcggat cgaacccttt
    20941 cacgaggttc gtcgaatggc agccggcgca catgccgttc cagttctggc cgttgcgcgt
    21001 ccagtgcagc cagtcggacg gcgggatgtt ccggcccggg tagaggaaga accaccgttt
    21061 tcccggcacg tcccacgcga gggagaacgc ctgcatccgc ccgccgggaa acctgacgag
    21121 aacctgctgg agcgggtccc agccgaacgt gtacgcggcc tcgtagaccg caggtttccc
    21181 gtcgggtccc gccgtctcga tcaggaactt cttcccgtca cgaaagaagc gcgctttcat
    21241 cccgtcgccc tcgaagacgg cgccggagaa gtcgccgcgc acggtcgcgt cggtcgcctc
    21301 cgtcatcgcg agatcgtggt gcgagcccgt ccacgccttc gtggctttct cgtggcaccg
    21361 ggcgcacacc ttgcggccca cgaaggcggc ctcggcggga gccgtgctct tcggcgcggg
    21421 cctgcggaac gaatcgcgga cgagcgcgag cgggcacgag gcgaccacca gggccgcggc
    21481 gacgacggcc gccagccgcc accgggcgag cgcgtccgtc accgcgtcac gacctcgtca
    21541 cctgcgcggc gtcgaacgag gaccggcgga tgggggccga gacgtcgacc ttcacgatgc
    21601 cgttctcgat ccgcaccgcg aagaggtcga gcgggcgggg cgccggggga gcgaggacgt
    21661 cgccacgcag gtcgaacacg gacgcgtggc acgggcacgc gaagcgtccg gcggcctcgt
    21721 cccacgggac gctgcacccg aggtgcgtgc actggcggtg gagcgcgagg aagccgccgt
    21781 cggcgaggcg cacgaggtag aacttgcccg ccgggaacgc cgtcaccgag ccgggcgcga
    21841 acccgtccga gggcccggcg acgacgaggc ttcccgcgac cttcggcgcc ttcggacgcg
    21901 gccgcaggaa cgccacgacg atccacgagg cctcggcgag cgccgcgatc ccgagagaga
    21961 gccacgcgag tttcaggaag gaccgccgct ccgtcacgcg ttccacggcc acaccagctt
    22021 catgcccgct ccccggaacc agattccgac gacggtcagg acggcgaagg ccgtcccgaa
    22081 gaagacgaag agagcctgcg cggcctcgag ccgcgaggcg ccgaagcgcg accgcaggag
    22141 gcgaaagccc gcggcggccc ccaagaggag cgcggccgcc ggcaagacgc cgcgaagaac
    22201 gaacgcggcg cctcccgagg gcgcacctgg cttgccgagt aggagcgcat ccgcgaggac
    22261 gagaagcggg gtcgccccga gcgcggccgc ggccgcgagc ttcgccgtgc gccggccggt
    22321 ctccgagagg aaccatgcgc cgcgcggctc gtcgtccggg aagccgagga acggcagagc
    22381 gcacaatgcg gcgatgccga gcaacggcac gacgacgacc gcgaagagcg gatggaggtg
    22441 gacgaggagc tcctggaggc cgaggaagta ccacggcgcc ttcgcggggt tcagggagag
    22501 gccggggttc gcggcgggtc cgagcggcgc cctcacgagg gccgagaaga gcgtcacgga
    22561 ggcgaggacg acgagggcgg ccgcgagctc ccgccagagg agctccggca ggaagaggac
    22621 ctgtgcgggt ttgccgttct cgtcgcgctc cgcgtcgggc ggcaggacca cgccgccggc
    22681 cttccgcacg cgccagaagt ggaaccccgc gccggcgaaa agcagcacgg gcagccactg
    22741 ggtgtggagc gcgaagtacg tcccgagcgt cgccggcccg atctcctcac cgccgcgcag
    22801 gaacgcggcg agcgcgggcc ccgcgagcgg gacgtaggcc gccatcccgg tcacgatcgt
    22861 caccgcccag taggcgagct ggtcccaggg cagcaggtag cccgtgaagc tcgacgccgc
    22921 gatcgccgcg agcaggaaga ggccgacgag ccagttcagg gcgcgcgttc cgaggaagcc
    22981 gcccgtgaag acgacgcgca gcaggtgaag gaccacgacg aggacgagga ggttcgcgct
    23041 ccagtggtgg accgcacgga cgaatgcccc gaacgtcacc tcggactgaa gcttctcgat
    23101 cgacgcgtac gcgtcaccgg gcacgggcgc atacccgaac atgaggagcg agcccgtggc
    23161 aaggaggatc gaaaggagca cgagcgacga cccgccgagg ccgaacgtgt gcgtgaagcg
    23221 gagcgtcgcc gccggaacgc gaaccgggcg gaggtggagg acgaacgtcg agacgacggt
    23281 ccgcgcgctc tcggcgggct tcacgccttc acgatagcgc acgcgcacgg ggcaggggcg
    23341 ccagggcgcc atgccccggc cccccggccc ggaaatcgcc gcgggaaact acttcttcgc
    23401 ctgcggcggg tagttctcga agagcttctg ggcgacctgg tccgccttct gctggcgttc
    23461 ctcgggggtc gcggaggtcg aaatctggtc cttggccatg ccgcgccaga cggcggtctt
    23521 cgtgctcgcg tcgacgaggt cgatgacgac ggtccccgtg gggatctcct ggaccgtggt
    23581 ggtctgcatg ccgcccatgc cgccgtagcg ccagccgccg ccccagccgc catacccgta
    23641 cgagttgagc tggacgtcct tcccgaggct gaagtgggtg aagaccgtca ggtctccccc
    23701 ctccggtacc tggcggaggc cgcgggccgc gagggcgttc ccgagggact gagcgaagcg
    23761 ctcggccgcg atcgggttcc tgggagccgt accggccttg agcgtgaacg tcttgtactt
    23821 cgagaagtcc gtgcccggcg cgtagtccgt gttgacctca agcgtcgagc aggcgaccgc
    23881 ggtgacgacg aaagccaggg tggcaaaacg gctgagtttc atgacagttc tccttcgtct
    23941 ctcggcttca cgatacttca taaggctcgg cgctcgggcg ttctccccga ggagtgcgcc
    24001 gcgtaatatg cttcccgatt ctatgaaagg actcgctatg aacaaacgca tcctcgcttc
    24061 gacgttggcc gttcttcttc tgtcggccgc acttccgctc accgcgcaga tggagcccaa
    24121 gggttccctc cagggcctgc agcccccgaa ggcggacgtt ccccagctca tgaccctcca
    24181 gggcgagttc gtccggatgg cctacaacaa cgagggctac gccgtcctcg gttaccgctt
    24241 ggtccaggac gaggtcggga agccctgggt ccttctgaac gtcggcatga cgctgcagga
    24301 gggtgtgaag aactacaagc tgaagcgcga gcacctcacg ctcaagacgc ccgacggcac
    24361 ggtcgtcccg ctggcgacgc agcaggagta catggccgac ccctatctgc gcgccctcaa
    24421 ccagcgcgcc aaggtcgtga aggactccat caactacttc ccggccggtc cgggtcgtcc
    24481 gtgcgccatg cagttcttcg ccgacctgga caaaccgggc ctcgcctaca acgaggtcga
    24541 catgaccgac agccgcgact gcatgggccg cctctacttc aaggtccccg gcggcatcca
    24601 ggtcggccag tactggctgc aggtccagtt cgcgaccagc atgatccagg tccccttccg
    24661 cgtcttcacg aaggagcagg aaaaggactt ccgcaagcag tgggaggacc tgaagaaggc
    24721 ccacgacgag acgttctcca tgtagcctgg tggccaagtc cctcgacccg gcggcggcat
    24781 tcgtggcccg ggcggcgcgc gccgccggcc tcgtcgccgc cgcgccgctc gtcgccgagc
    24841 cgctcgcctt cgcgtccggc ctcttcaggc tgcaggggcg gctcgccgcg gccgtcaacg
    24901 cgcgccacgt ggacgccgcg ttcaccggag ctccggccga cttccccagg gtgctcgacc
    24961 tcctcgcgcc tctcctgacg tacgcggccg aaaaaggccc gcccgagctg gccgaggtcg
    25021 cgagggcacg acacgccgac gccgacgcga cggcgctcgc ccgcctctcg gtctactgga
    25081 gcggcgaacg cgaggcgcgc gaggactacc tctcgcgcgc gttcctcagg ccctacgccg
    25141 aggtgctcgc cgcgcgcggc gtgacgctcg aacgcccgcg ccacgagggc cactgccccg
    25201 cctgcgggaa cggcgcggtc gtgagcgtgc ggcgcgaggt gcccgagtcg atgggcgcgg
    25261 cgcgttccct cgtctgcgcg ctctgcggca ccgaatggcc gttcgcgcgc atccgctgcg
    25321 ccgcctgcca cgaggaggac cccgcgaaac tcccgtcctt ccggacggag gcgcatccgg
    25381 cggtccgaat cgaggcgtgc gagacgtgca agcgctacgt gaagtcgctc gacctcacgc
    25441 tcgacgcgcg gccgctgccc gaggtggacg acctcgtctc gctcgcgatg gacctctggg
    25501 ccgtcgagga gggctggacg cggctcgagc cgggatgggc gggaatctga agccttccct
    25561 gctcgtactc gcgttcgtcg gcggagggat cggctcggcc tgccgttacg cgatgacgct
    25621 cgccctgcag cagaggctgc cggggccgtt tccgatcggc acgttctcgg tgaacctcgc
    25681 ggggtgcttc gcgatcggcc tcgtcggggc gctcggcctc gagcgcgccg cgctttcgcc
    25741 cgaggcgcgc acgttcctca tggtcggcgt cctcggaggc ttcacgacgt tctcgtcgtt
    25801 cgcgtgggag actctggagc tgatctctgc ccgcgacgtc ctgcgcgccg tcgtgtacgc
    25861 atgcgggtcg gtggtcctcg gcctcttcgg cgtgttgctc ggccgtgctc tcgggcgcct
    25921 ctagccgagc tgcggaaagt ccggagagga ctccgtctcg ggctcggcgg cgcgcagcgc
    25981 gtcccacgcc gcgacgagcc gctcggtcgt ccacccgagg aagacgccgt cgccccgcca
    26041 gagcgtcgtc tccggcgtga gctcgatgcg cacgatccgg ccgggcccct gcgcggtgtg
    26101 gacggagagc gcccacgccg agaacgtgtg ggcgacgtcg cgcgcccgcg ccgcgatccg
    26161 ctcgacgcgg tgggaagaaa ccaggattcc ggcgagtggg tcgaagtcgg ggtccgagct
    26221 catcgaccgg atcctagaac gaagcgtgga gcgccgctcc gccccccttt cggaggagca
    26281 gcggcgtcac cacgatggaa acacgtccgc cggatccgcg ccgccccgcg cgaatcgcgt
    26341 cgttcaccct cacgacggcg ttcccgacga ccgtgccgag gatcgcgccc gcgaggacgt
    26401 ccgaggccca gtgctcgttc tggtagacgc gcgcgaggcc gacggacccg gcgaggccgt
    26461 acgacacgac gctcacccag agccgcccgt cgtgcgccgc gatgaccgac gcgacggcga
    26521 acgcctgcgt cgcgtgaccg gacggaaacg atgcgtcccc cgagaacggc gcgaaatggt
    26581 cgggaccctc gccggcgttc gggcgcgcgc ggccgacgac gaacttcgag acgggcgcca
    26641 cgacgccggt ggcgatcgcg ctcgcgagga gaccgtccac ggcgacgttc atcgcctcgc
    26701 ggttcttccc gacgaagccc acgacggcga agccgccgag gacggcgaag gagcacccca
    26761 gcccgaagca cgcgacgacg gaagccgcgt cgtcgcgcgc ggaggtgcgg ctgcgctgtg
    26821 tggcgtcctg gagcgtgttg tcgaggagga accccgtggc gacgaccgcg gccgcggcgc
    26881 cgccgaactt gagccagtcc gtgccgtccc atttcagcgg ggcgaggagg acgcccgtcg
    26941 catcgcccgc cgccagcgcg cccgtctcct cgaacgaaaa gaccggcggc gggggcggcg
    27001 gcgccgtgga ggcgtcctcg gcgtgcgcgg acggggcgac gaggag
//
LOCUS       DAOMFY010000022        27038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_585_length_27038_cov_6.972464, whole genome shotgun sequence.
ACCESSION   DAOMFY010000022 DAOMFY010000000
VERSION     DAOMFY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27038
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_585_length_27038_cov_6.972464"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLB01_03560"
     CDS             <1..657
                     /locus_tag="PLB01_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NucA/NucB deoxyribonuclease domain-containing
                     protein"
                     /protein_id="HQQ76408.1"
                     /translation="YARNNPLKFVDPNGLQVASTQSTGQFLMAAGRQAMVSANGQAGF
                     RLLGTVLGGMSLAKLGDLLTMGTDTGTAVESGRNLGEALSADISRGIIIGSVLGGIAG
                     ALRSAAEGSITEISVSSSRFGEAAAHIEEAQAAGQPSVLTIDRAGASARRADAMRGVA
                     RVPGLDRDEYPPAMFKEGGEGASVRTINRSDNRACGAFIGNQCSGLSNGQTVRIVPSD
                     "
     gene            670..1293
                     /locus_tag="PLB01_03565"
     CDS             670..1293
                     /locus_tag="PLB01_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HQQ76409.1"
                     /translation="MTLKSLIDLSGPSLSDALPRRTLQSLGKRENELYELLRTKNGFY
                     AFESALHVFPSGPAKKAAITLEEWNDASTWRACFDGLGDGLFFAEDIFGGQFFIRGES
                     ILTLDPETGRVESFARNLEEWASRVVDDFEVVTGHPLAHEWQQQHGRLPADSRLIPKT
                     PFVLGGAYVVENLYASEAAKGMRLRSEIARQIRDLPNGAKVRLKVVE"
     gene            1652..3235
                     /locus_tag="PLB01_03570"
     CDS             1652..3235
                     /locus_tag="PLB01_03570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ76410.1"
                     /translation="MHLKGRLAAGGVLFLAALVRAPFVLPSLPYLAYMDEGHVLHHAA
                     VLARNGGWDPGWYGYPSLTLYLIAGAFRVLAFAFRIGGGRDLLSAVQPNAAFYDFVMP
                     PELIVVGRVVVLLAGLGIVALGMALARRLAGERAGLVAGILLALCPALVQRSPIVIVD
                     TVAAFFALGALFAALRVEGPAESSARETQVRWSLAAGALSGLAAASKYPVGAVMIAVL
                     GTIALLPAPALERIKLGAAAVAAACVGAVVGMPALVLKPAAVFEALREQARLYGDPAL
                     MFHGAAPVDRSAVRDSFRSYEMGAVLPFFGVAAFLVILALGKKQRRQVGPWIAFAAVL
                     LVSVLGFPFQPFRNLLVLVPLLVVAVPVAIDSALPPGRRRGLAGAATCVALAATFIPG
                     LLLIRQVYGRGDSRVTAVGRLAETLGPQDRVLVLRELAVLPAELARLGAPVTVVPWYE
                     ARAAAETGSFTRLVFGDLDPSGYVPGAGENLSELPAFRNWAAGLPEAFRVGSARTPMP
                     PVFWRSAEELLVVARLPHR"
     gene            complement(3243..4124)
                     /locus_tag="PLB01_03575"
     CDS             complement(3243..4124)
                     /locus_tag="PLB01_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76411.1"
                     /translation="MSVYATGLGAALRNRRLAAVLWLSLLGSALVTAGPMKALFRVLD
                     EGAFREALVKGWDGWAFLSFLWNRRPEIGIALAAVSAAFVVFALLTIFLTGGVLRALI
                     AGRPRPVFRLVVAESAGLFKANLWATIRFAVAAAFWLGLLVALPVYGIHKLFKDAAPH
                     TFWPNLSFWWRYGVGAIVLLNVGLRFDLARVALARGEAVNARGAYRVAKRRLRGSRPS
                     AILLAVVWIAIGLAVQAAFTAWGLRWNPHTDGSTFGLFVFRQFGFFFLAMVRVGFWAS
                     LLAWENERRPSPSQYGT"
     gene            complement(4121..>4477)
                     /locus_tag="PLB01_03580"
     CDS             complement(4121..>4477)
                     /locus_tag="PLB01_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76412.1"
                     /translation="KRKDDKDKDDKGTWQSLVTVGRDGDIEMPAKVVLTFKDRTTVTR
                     TWDGASGWIKYRVTSSSPLVKAEVDPERRTVLDRNPWNNARYTKKYEGPSAAAKGRTY
                     ALHLVEILLSTLWGVL"
     assembly_gap    4478..4577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4578..6068)
                     /locus_tag="PLB01_03585"
     CDS             complement(<4578..6068)
                     /locus_tag="PLB01_03585"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQQ76413.1"
                     /translation="MRQLALAAAASLALALPASALEAPRKIVDYDIKVSLDPETKLVE
                     GSETLKWTNPSDGPVGELRFHLYWNAFRNDRSTFFRESGGQLRGDKADTGKGWGYTDV
                     TSMKWDGAELMKGARFESPDDGNPDDRTVLVVPLPRPVAPGETATLAIAWKAKVPKVY
                     ARAGYVRDFFFFGQWFPQIGVYEPKGRRRRAEAGWNCHQYHANSEFYADWGDWRVAIT
                     LPEKFVVGSAGALVSEKREGGKKTLVYEQKAVHNFAFTADPRTVVVEDVFDPAKDIPK
                     DELALAAKTLGRTEADLLAGFRKVAIRLYRQPEHGAFDRRHIEAQKWALAWTGLWAFP
                     YPYAQISIVDTPEDGTGATGMEYETIYTTLSYKWMARWPFDRLYIPEMVTIHEFTHGY
                     WMGLVASNEFEESWMDEGINTFTEIVMVDRKYGAFLTVPPGIGFKNEESNRAQAALST
                     NHDPILSASWKFATEGSYGRNSYPQTGTTVQQIRLLLGEETFWKAFR"
     gene            6107..7126
                     /locus_tag="PLB01_03590"
     CDS             6107..7126
                     /locus_tag="PLB01_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ76414.1"
                     /translation="MRAGDLVSLTGGTGFVGSHVADALLAAGYRVRALVRRPDDVAWL
                     KGTGVDLVKGDVRDAATLPALVEGASAVVHVAGKTSARNEAEYMAANAAGTENVAAAS
                     RRHAPGAHVVLVSSQAAGGPSVGGVPVKAGDQPRPVSAYGRSKLAGEEALRRSGVPFT
                     ILRPSGVYGPRETAIRDLFVAASKGVVPILAGGRPRIQLAYGPDVAAAVAGALERGPR
                     GETFYVAHPEVLDYGSIAETLSRLPSRKPWRLPVPSGVVRGAGHLAGAVSRFGKGPPV
                     FNAEKANEMLQEAWLCDVSDAQAALGQPFRTDFATGSRLTWDWYLERGWLVDRGDKIP
                     LRETR"
     gene            7171..8403
                     /locus_tag="PLB01_03595"
     CDS             7171..8403
                     /locus_tag="PLB01_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HQQ76415.1"
                     /translation="MSARTHGGSAASANRSIFEKCRTYTAPDDLRAAGLFTFFRMIES
                     GQDPVVMMGGREMVMLGSNNYLGLTSHPKIKEAAIAAVRKYGAGCAGSRLLNGTLDIH
                     VQLEERLAAFMKKPACVTFSTGFLVNLGVLSSLASKGDTIYLDRQDHACIFDGARLAV
                     GADIKKFKHNDPADLRRLLAIGGDRSGGRMLVIDGVFSMEGDIAPLPEYADICDDYDM
                     ALMVDDAHGVGVLGKHGRGTAEHFGIEDRTDIIMGTFSKSLATVGGFVVGEPDVIKYV
                     KHRARALMFTAAPPPASVAAVLAALDIIESEPERRDRLWENTRFMLTALRAIGFDCGE
                     SSTPVIPVVVGPDEVAFKMAKMLENEGVFVNAVVSPATPPGRALIRTSYMATHTKAHL
                     TRAVEAFHKVGRELGLVS"
     gene            complement(8432..8821)
                     /locus_tag="PLB01_03600"
     CDS             complement(8432..8821)
                     /locus_tag="PLB01_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQQ76416.1"
                     /translation="MIRRSPQPLFAPIAVALAGFLATACNEAKSPTEPVAFVAKTPTP
                     APTVVAGEPAIMAGIVSAYAGTPPVAGAIVECQGKSTTTASDGSYLLGGLVSGTWTVT
                     VRYGGDSSQDQSVVLRPGTNTVNIGLI"
     gene            complement(8814..9479)
                     /locus_tag="PLB01_03605"
     CDS             complement(8814..9479)
                     /locus_tag="PLB01_03605"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQQ76417.1"
                     /translation="MTFALLGIACTETKTPTEPTAVVAATPTPAGPAVTLSGVVTDQS
                     GAPYSPAVVYCQGVGSTEVSADPRGKYRLTGLKAGAAVVKVYAQGQQKSQDFDVVLAV
                     GDNVKDLAINIVTGEPASMSGFVRLPSGEPAPSAILSCQRKGVNIGADGSYALTGLYA
                     GRWNVSLTWNGYNDEEYETVTLRPGANTVNFVVGAPPPAAWMSGIVTSRFPSEETREQ
                     HHD"
     gene            complement(9551..9967)
                     /locus_tag="PLB01_03610"
     CDS             complement(9551..9967)
                     /locus_tag="PLB01_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76418.1"
                     /translation="MTTWGPGGPSFGTFTLTYLRAGKTTLDVYPPGQTIPTSFEVELV
                     PGTNTKNVSITVVHGQPASLSGRIIPQSSRPIAGITLYCQGRSAPVAADGSYALAGLE
                     SGTWEILIGWDVYLGEFYTNVTLGPGANTQNFNIPY"
     gene            complement(10218..12167)
                     /locus_tag="PLB01_03615"
     CDS             complement(10218..12167)
                     /locus_tag="PLB01_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76419.1"
                     /translation="MRIRDRFSAARRAPLAALAFLTARTAAAAWTALGPPIPAIEGAV
                     AVHPASGTVYVGTFGGGILKSLDGGATFAAANRGLSDLALGVTSLAMDPTDPDVVVVA
                     SAGGGIFRTSDGGRTWSPTSDVDTNVVSVAVDPLDPRVWYAGYGVGSSASLSKSTDRG
                     ATWVKSDAGIPPTTIWSIAPDPHRAGVVYAASGDSGAFKSVDGGTTWAALAVQPVVWA
                     IAVDPTRPDVVYAGVNGDGVFKSTDGGATFTRVGSPDAGVVLALAVDPTAPDRVWAGT
                     ISGGLAVTEDGGVTWRPTSVRSGNVLSLTVTADGDVWAGTGADGVLTTSGGDADVPGL
                     PARRRRYRLRRAGLDALSAINAQNAVSLSVDPRDSSRLMLSTNDGGLLDSRDGGRSWS
                     QAGRGFLSRASRRAVFDPRKPARILVGSFNGGGLYVSDDGGANWTRRVFGSPGVYVWI
                     SAVDPLTGTIWAGTRGEGLWRSVDDGAHFTRVTSLTMPQVRAIAVDAARPGRVLVGGN
                     TGIWRSTDGGASFKRSSTTFTLSLTVDPLDPDVIWAAAQTAGVSRSTDGGLTFSPRSS
                     GMTFTRMSRSGVVAVDPSNPSVVYAATEGGGVFKSRDAGASWFSVNAGLGDLNVYGLT
                     LDPKDPNVLYAAGPHGVYKTETGAE"
     gene            12300..14687
                     /locus_tag="PLB01_03620"
     CDS             12300..14687
                     /locus_tag="PLB01_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76420.1"
                     /translation="MPPLDAGSKVGPYVVVARLGTGGMGEVWRARDERLDREVALKVL
                     PSGFLEDEERRLRFEREAKLLASLSHPNVATLYAFEEIPASADSPRRHVLAMELIEGG
                     TLRRLLDAGRPPLRRALRIAAAIADGLAATHAKGIVHRDLKPENVAVTDDDIVKVLDF
                     GIAKSSPLAAAGRDDTLPEAALGTEPGSFLGTVGYMSPEQAAGESVDYRSDQFAFGTI
                     LYELATGERAWTKPTAAETLVAILREEPPRTGALPAALRRIVERCLAKDPAARFASTR
                     DLARDVRDLLEGTDAVPEVTVAPRVRSRTRLPRFAWAAALAASAVVAFFLVRGRLETK
                     PVSSIAVLPFEGGPGGETEDYVRDGLTETLIERLSRLPDVRVMARATVFRFRGSTDPV
                     KAGKALGVSAVVTGSVARRGDRILVSAEAVDARTGARLWGDRYDRPVVDLVRVEAELA
                     SDLAAGLRLRASEPERRALARPGTSSSEAYDAYLRARFASLQDTEAGYLEAIRLFRAA
                     TEKDPSFAEAHLRAADTYGEMASGGYLPPAEAWARSDEGARRALVLDPDLPDAKAALA
                     ARRAYYDWDFDGAERDFRELFDGPRPPVWHARAFALLLWARGRTEEAIERTERARTLD
                     PGNVVFTVASGDYRAQAGRLGEAVGLYRAAIDAEPEDPRAWFGLAAIFRSRGEAARAI
                     ESLRKAYAFAGEKDGAEALAGASTVADYDAVQLRVARSRLAEAEALARTRYVSPLDTA
                     RLAAMAGEKERALAGLGAALKERSPGLVFLNVDRVWDVVRADPRFSAVVRKVGLS"
     gene            14740..15381
                     /locus_tag="PLB01_03625"
     CDS             14740..15381
                     /locus_tag="PLB01_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ76421.1"
                     /translation="MATTTSSRRVPLGAVAFDPPRRVLVDRGAVVALSRLEYALLSLL
                     VERRPRAVSKDEIQEILWPDVAVSETSLTTLVKDLRAKLRQSGREGPIRTVHGYGYAL
                     EGAPGAGPAPPRLVRGRTEIPAVSPDLVLGREPGKPGSIDDASVSRRHARLTWNASGL
                     VLEDLGSKNGTYVRGERITGPVALKDGDDVRLGLVTFVFRAAPTGASTTKTVA"
     gene            15390..16529
                     /locus_tag="PLB01_03630"
     CDS             15390..16529
                     /locus_tag="PLB01_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQQ76422.1"
                     /translation="MNAIQIDRVDSSRALRDFVDLPFRIYADDKDWAAPLKSDVRWML
                     DLAKNPFWKHAKRELFLARRDGKVLGRIAAIVDDEHNRVHGEKIGFFGFFESEDDREV
                     AAALFRAAEAWVRGVLPEASLLRGPVNPSLNDEAGALVPSESEPGLPFIMMTYNPAYY
                     LDLFGAAGYAKVKDLVAILAPVDDRSFERLRRVTDIVKKREKGRAVVRQIRMDKFDED
                     LAIVKDLYNRSWEKNWGFVPMTSDEIDAMAKKLKPILYPPWVLFVELDGKPVGFHLAL
                     KDYNQVLKELGGSLFPFGWLKFLLLQKKIDRVRTLALGILPEYRRRGFDALLYFEAVK
                     EGVRLKYTAAEFSWLLEDNLDIQRPLEVFGGRIYRRYRIVERSLA"
     gene            16572..18983
                     /locus_tag="PLB01_03635"
     CDS             16572..18983
                     /locus_tag="PLB01_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76423.1"
                     /translation="MALQPGDRLGPFEVEVLLGVGGMGEVYRGRDTRLGRDVALKVIS
                     PKRVGDADLRRRFELEARAASVLNHPSIVTIYDVGETNGVSWIAMEWVEGRTLREVLA
                     DGPLPMRDVLTIGRQIAEGLCAAHERGIVHRDLKPENVMVGRDGRTRILDFGLARQTF
                     AGALSGGSSAIETLATPAAVTGEGVILGTVGYMSPEQASGLPADARSDQFALGLIVYE
                     MVAGRRAFARATAVETLTAIIKEEPPPLSTVRLDVPPALESVIAHCLAKRPEKRFEST
                     REVAAALGTDATGSAPISFSPTLAVVDAPPLERRRLPLRRAVVVIGAVALLAGGVAAY
                     RARAAKGAIRSLAILPFENASKDPEVEYLGDGLTDNLINQMSRVASLKVMARATVFRF
                     KGSLDPQDAGKKLGVGAVLTGTVLRRGDRLSISVELVEVPTGARLWGEKYDRPSSDLI
                     RTQDDIAADISDGLRLRLSGAEKSVLGRHGTENSEAYDLALKARYFVLKDTEEGYLEG
                     RRLYLLAAEKDPAFAEAWLGVSNTYGVMAVNGLLAPAEAWARSDEGARKTLDLDPGNV
                     GARCALVTRRFFFDWDWAGTEHEFRELGADPRVIRGEGLRPIGLSLWARGRADEAFVL
                     MERAMRLDPGNVAAMIATGDYLAQAGKLAEAADAYGAAMKAEPSDSRPLFGLSDVLLR
                     RRDVTGAIDALRRAYELDGEDEGVRLLASARTENDLDTAKIGVARSRLAEVEEMSRKR
                     YVSPLDVARLNAQAGEREKAFASLEAAFKERSPGLVLLRVDRAWDRIREDPRFTDLVR
                     RVGIP"
     gene            19071..19601
                     /locus_tag="PLB01_03640"
     CDS             19071..19601
                     /locus_tag="PLB01_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76424.1"
                     /translation="MRARSVAFSAVAFAGLLAGADLLACGDKFLVVGRGTRYQRPKGA
                     RAASVLFYASAESGLSAMLKAMPVDSVLKREGHRGTSVTTPEQLSAALAGGHFDVIFA
                     DAADASTVERLLAGRADAPTLLPFCDGTKSTSVVVDQKGAFCVTTPPKERSMLEAIDQ
                     AVERRDRRVAKPAIRS"
     gene            19618..20544
                     /locus_tag="PLB01_03645"
     CDS             19618..20544
                     /locus_tag="PLB01_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76425.1"
                     /translation="MRRFLPRAVLAAVVAAASPRLDAQAFTLPRGVAAFTIVWQYVDN
                     TGHRMTDGYVLDQGQSLSTGILAELDYGVTDRFSATVGIPYIFAKYTGGPPPPGIPYL
                     PVDSCNCWHSGFQDFAFTARYRFGDDPWAVTPLARYVLPSHSYNYQGEAVIGHDLQEF
                     QLGAFASLRLVTLLPDANIQVGYVYSFVEKVLGIPNDRSNTSFEIGYLFGRRFYVRAA
                     GYWQVTHGGLRLGSVTGNPFPITGEVNTPELGAEHDRLLRDNYWRVGGGVSYSAGPFD
                     VFASISKYVAGTDTHNGQAYTVGMTWYFGTPK"
     gene            complement(20545..21420)
                     /locus_tag="PLB01_03650"
     CDS             complement(20545..21420)
                     /locus_tag="PLB01_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQQ76426.1"
                     /translation="MRWKPVDALTSLFAVGLVVAGLVRYQALESPGVLLRMGLVALVP
                     AVAAWLRGRNPNPSKNLQILLDYYVIACIVVIFDGLGPLIRAVNPVDKDPALIAFDRW
                     LTGTDPTVYLERFATPFLSDVLTFFYSLYYFHPIVLGTLVLLDDRERPPAARDFSRYA
                     FTMVFVFFVSYVGYFIVPAVGPRFTVTHAGPLPRGAVARVIDDTLNKLESNKRDCFPS
                     GHTMVVTVVLLEAARRSRTTFWWFLPFAVGLVIATVFCRYHYVADVLAGLALAFVAVP
                     LGNALYRRFGDPAAG"
     gene            21457..22767
                     /locus_tag="PLB01_03655"
     CDS             21457..22767
                     /locus_tag="PLB01_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HQQ76427.1"
                     /translation="MRCAAVLFLSGALAASAAPAPPSAAGTVVVADLNTEIQAVSAAF
                     VARVLKDAEKNAAPFVVLRIDTPGGRIDSTRTITQAILASPVPVVGWVAPPGSQAASA
                     GFIILMSCDVAAMAPGTNAGAAAPVGGGGEDLPKTLAKKAGQDVSALLRSVTEPRGRP
                     VDDAVKAVSEAASYSESEALKRKLIEIVARDEKDLLAQLDGRTVKRVGKSDAVLKTAG
                     LVTATRTMTRRERILCVVANPGLAGILFLIGLVGLYSELSHPGGILPGVLGAIALLLA
                     LFAMSVLPVNAAGVALLLVGALFFFLEVKLASHGVLAVGGAAAVVFGAILLFPDQAGA
                     PRGEFAVLVAGAVATAAILAVLSFKALAMKSLPDRTGLGALVGQIVSARSPIAPVGKV
                     FADGALWEAHSSVPVAAGDLVEIVGIEGLALVVRPARKAGQEPG"
     gene            22764..23522
                     /locus_tag="PLB01_03660"
     CDS             22764..23522
                     /locus_tag="PLB01_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HQQ76428.1"
                     /translation="MNNLTFLVPIVFVAIIFISKWINILNEYERAVTFWLGRLSPQPK
                     GPGLTFIFWPFETMIRISLRTVVLDVPPQDVITRDNVSVKVNAVVYFHVMDPSKAVVK
                     VENYLYATSQLAQTTLRSILGQATLDELLSSRDKLNENLQEILDKHTDPWGIKVTMVE
                     LKAVDLPIEMQRAMAKQAEAEREKRAKIIHASGEFEASKQLSEAAEIIGREPATLQLR
                     YLQTLTEIAVEKNSTIIFPVPMDLISVFMKKDKA"
     gene            23522..24190
                     /locus_tag="PLB01_03665"
     CDS             23522..24190
                     /locus_tag="PLB01_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQQ76429.1"
                     /translation="MAGLGLEDRHEEMPERAAPVHYQISITGRQAGFFFLLLLGALGL
                     SFFFGMKTGAAAKKGPGGGAAGVIASASDIPVPTLAPAESLREAKRPDDTLGIKPEEK
                     RAAEAAPPPSPAPTAAPTAVPPTPTAAPVPTPKPAAAAKPAPKGPFYVQILATKDAAR
                     ADELAKRLKDEGFNADVAVIAGKPGWFRVHVGPFKDRAKAEALAKKIKATDKQIKNVP
                     LVVP"
     gene            24187..25062
                     /gene="lipA"
                     /locus_tag="PLB01_03670"
     CDS             24187..25062
                     /gene="lipA"
                     /locus_tag="PLB01_03670"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQQ76430.1"
                     /translation="MSRPLPVRGEPALPPWIREKKLRLDDLHAIKARMRLGKLSTVCE
                     EARCPNRTECFERKTATFLIAGDVCTRACGFCHIASGKPAPLDPAEPASLAAAARDMG
                     LAHVVVTSVDRDDLPDGGAAHWAAVVRALKEDDGARTVEVLTPDFQGVAAQIDCVADA
                     GPDVYNHNVETVPRLYDAVRPKSDFRRSVDLLARVKARHPGMTAKSGLMLGLGERDDE
                     VLDVFRALRQAGVDVVTVGQYLRPSAWNLPVAEYATPERFAALGRGGMEMGFTAVFAG
                     PFVRSSYRAGEFLRR"
     gene            complement(25136..25678)
                     /locus_tag="PLB01_03675"
     CDS             complement(25136..25678)
                     /locus_tag="PLB01_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76431.1"
                     /translation="MKRPARTLAALFPVALLAAASLVPSSLIFGAERPQTIRPVPTPA
                     LSARSTISDVQAAYDAMEKAIEREYWKLIQEARSEKSSLENQIKELEKQVETLKAQAK
                     ADAGNAAKLAQINQALESLKQKIKALKSQIKLLDQEIEKLEKQKDAEISKLRKQKATA
                     LEQAAKSRPASTPTPTPARK"
     gene            25755..>27038
                     /gene="lnt"
                     /locus_tag="PLB01_03680"
     CDS             25755..>27038
                     /gene="lnt"
                     /locus_tag="PLB01_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQQ76432.1"
                     /translation="MLPSLPSKKFPRLLFSLASGVLLALCFPNFSILPLLPVALIPLI
                     AALSGLTWRKAFLPGFVFGAAFWLVTIPWIAYTVNRFGGVGPVLAGLALAITATIYAV
                     PFGLMAALYAAARPRTGPGIVAAFASAWVAQEGFRTYIYVFGGFPWNLLANPLADVPA
                     LLGSTALGGVLFTSFLLAALNAALFVAWTRPGRTERLGWLLGAAVCALAAAAVRAPRP
                     RPSDEVLKVGVVQPNVEQDIRWDRGTAERIQTDLEDQTRALCRADRPVLVLWPESASP
                     YSWSFNALYRERVLRLCRELDVAILMSTAWSDAPWAEDAPYYNAALLVTKDGPVLPPY
                     FKQRLVPFGEYVPLAPLLRMIKPISRAVPGGFTPGSGTVLLKLGSWNLGGAVCYEVVY
                     PWIARAEARAGADVLFTLTNDAWYGRAGAQRQHWQA"
BASE COUNT         3998 a         9184 c         9412 g         4344 t
ORIGIN      
        1 tacgctcgaa ataatccact caaatttgtc gacccgaacg gattgcaagt tgcctcgacg
       61 cagtccacag gtcagttcct aatggccgct ggaaggcagg ccatggtgtc ggctaacggg
      121 caagcgggat ttcgcctcct tgggactgtg ctcggcggga tgagccttgc caagctgggc
      181 gacctcctaa ctatgggcac tgacaccgga accgccgttg aatccggaag gaaccttggg
      241 gaggccctta gcgcggacat ttctcgcggg atcataatcg gctccgtcct gggtggcata
      301 gccggcgcgc ttcgttctgc cgctgagggg tcaatcaccg agatttcggt gtcctctagc
      361 cgttttgggg aagcagccgc ccatatcgaa gaggcacagg ctgcgggtca gccgagtgtt
      421 ctgacgattg accgtgcggg cgcttcggct cgtcgcgccg acgcgatgag gggagtagca
      481 agagtgccgg ggctggatcg cgacgaatac ccacctgcaa tgtttaagga gggaggtgag
      541 ggcgccagcg tacgcaccat caaccgcagt gataaccgcg cttgcggggc gtttatcggg
      601 aatcagtgtt ctgggttgtc gaatggtcaa acggttagaa tcgtgccctc cgattgacct
      661 cggaccgata tgacgctcaa gagcctgatc gatctctccg gcccttcgct gagcgatgcg
      721 ctgccgaggc gcactcttca gtccctgggg aagcgggaga atgaacttta cgagcttctt
      781 cgaacaaaga acggtttcta cgccttcgag tccgcactgc atgtctttcc ttccggcccc
      841 gcgaagaagg cagccatcac cttggaggag tggaatgacg cttcaacttg gagggcctgc
      901 ttcgatgggc tcggggatgg cctcttcttc gctgaggata tttttggagg ccaattcttc
      961 attaggggcg agtcgatctt gaccctcgat cctgaaacgg gtcgcgtcga atcgtttgcg
     1021 cgaaacttag aggaatgggc gagccgcgtg gtcgacgact tcgaagtagt cactgggcat
     1081 ccactcgctc acgagtggca acagcagcac ggtaggcttc cggcagattc ccgcttgatc
     1141 cccaagacgc cctttgttct cggcggcgcg tacgtagtag agaacctcta cgcttccgaa
     1201 gcagcgaagg gcatgcggtt gcgttcggag attgcgcgac agatccggga tctcccaaat
     1261 ggtgcaaaag taaggctgaa agtcgtcgag taaggctatt caccgtcacg acgccccggc
     1321 gcgttcggcc gcgcggggaa gaggatccgg acgctcagcg ccgcgcgtgc ctggcgggct
     1381 cctccccggc cctcctaccg tgcacgcgcg gcgcatcccg ctccttgaat ccgtgggtcg
     1441 atgtccgata cttcgacggt gtcgaacccg gtgcttttgt tttccctcgc cttcttcttc
     1501 gcgccgaggt ggttcccgcg cagcgaacgg gtcgctgacg cgactcttga gtgtgcgaac
     1561 cctacgtacg caagcgttgc ttcgctcggc caaggcgccg ggcgagctgt ggaaggtgcg
     1621 attcgttgct tgagtgcgct aacgtcggta gatgcacttg aagggtcggc tcgcggccgg
     1681 cggcgtcctc ttcctcgcag cgctcgtccg ggcgccgttc gtccttccct cgctgccgta
     1741 cctcgcgtac atggacgaag gccacgtgct gcatcacgcc gccgtgttgg cccgaaacgg
     1801 gggctgggat cccggctggt acggctatcc gtctctcacg ctctacctga tcgccggtgc
     1861 gtttcgggtg ctggcattcg cgttccggat cggcgggggg cgggacctcc tttcggcggt
     1921 tcagccgaac gccgccttct acgatttcgt gatgccgccg gagctgatcg tcgtggggcg
     1981 cgtcgtcgtc ctgctcgcgg gtctgggaat cgtcgcgctc gggatggccc tcgcgcggcg
     2041 actcgcgggg gaaagggccg gactcgtcgc cggcattctc ctcgccctct gtccggcgct
     2101 ggttcagcgg tccccgatcg tcatcgtgga cacggtcgcc gccttcttcg cgctcggcgc
     2161 actgtttgcg gcgctccgcg tcgagggacc agcggagtcg tcggctcggg agacgcaggt
     2221 ccgttggagt ctcgcggcgg gcgctctctc gggactcgcg gccgcgtcga agtatcccgt
     2281 cggcgccgtg atgatcgcgg tcctcgggac gatcgcgctt ctccccgctc cggcgttgga
     2341 gcggatcaag ctcggtgccg cggcagttgc tgcggcctgc gtcggtgcgg ttgtgggcat
     2401 gcctgctctc gtcctgaagc cggcggccgt attcgaggcg cttcgcgaac aggcgcggct
     2461 ctacggcgac cccgctctca tgttccatgg cgcagcgccc gtggaccggt ctgccgtgcg
     2521 agattccttc cggagctacg agatgggggc agtcctgcct tttttcggcg tcgctgcctt
     2581 tctggtgatc ctggcgctgg gaaagaaaca gcgccggcag gtcggaccct ggatcgcctt
     2641 cgcggcggtc ctcctcgttt cggtcctcgg tttcccgttt cagccgtttc gcaatctcct
     2701 cgtgctcgtg ccgctgctgg tcgtcgcggt tccggtcgca atcgattcgg cgctcccgcc
     2761 gggcaggagg cgcggcctcg cgggcgccgc cacctgcgtg gccctggcgg ccaccttcat
     2821 cccgggcctt ctcctcatcc ggcaggtcta cgggaggggc gattcacgag tgacggcggt
     2881 aggccggctc gcggagacgc ttggcccgca agaccgggtc ctcgtccttc gggagctcgc
     2941 cgtcctgccc gccgaactgg cgcgactggg tgcgccggtc accgtcgttc cgtggtacga
     3001 ggccagggcg gcagccgaga ccggcagctt cacgagactc gtgttcggcg acctcgatcc
     3061 gtccggatat gtgccgggcg ccggcgaaaa cctctcggag cttccggcct tcaggaactg
     3121 ggcggccgga ctgccggagg ccttcagggt gggatcggcc cgcaccccga tgcctccggt
     3181 cttctggcga tcggccgaag agctcctggt cgtcgcgcgg ctcccgcacc gctgacgacc
     3241 gactaagttc cgtactgcga cggcgacggc cggcgctcgt tctcccatgc gaggagcgag
     3301 gcccagaagc cgacgcgcac catcgcgagg aagaagaacc cgaactggcg gaagacgaag
     3361 agcccgaacg tggagccgtc cgtgtgcggg ttccagcgca ggccccacgc cgtgaacgcc
     3421 gcctgcacgg cgaggccgat cgcgatccag acgaccgcga ggaggatcgc ggacggccgg
     3481 cttccgcgta ggcgccgctt cgcgacgcgg taggcgccgc gtgcgttcac cgcctcgccg
     3541 cgggcgaggg ccacgcgcgc gaggtcgaag cgcaggccga cgttcaggag gacgatcgcc
     3601 ccgacgccgt agcgccacca gaacgagagg ttgggccaga atgtgtgcgg cgccgcatcc
     3661 ttgaacagct tgtggattcc atacacgggc agcgcgacga ggaggccgag ccagaaggcg
     3721 gctgcaaccg cgaagcggat cgtggcccag aggttcgcct tgaagaggcc ggcggattcc
     3781 gcgacgacga gacggaacac gggcctcggc cggcccgcaa tgagcgcgcg caggacgccg
     3841 cccgtcagga agatcgtgag gagcgcgaag accacgaacg cggccgaaac ggcggcgagc
     3901 gcgattccga tctccggccg ccggttccag aggaaggaca ggaacgccca gccgtcccag
     3961 cccttcacga gcgcttcgcg gaacgcaccc tcgtcgagca ccctgaagag ggccttcatc
     4021 ggcccggccg tgacgagcgc ggacccgagg agcgagagcc agaggacggc ggcgaggcgc
     4081 cggttgcgaa gggcggcgcc gaggccggtc gcgtagacgc tcacaggaca ccccacagcg
     4141 tggagagcag gatctccacg agatgcaggg cgtaggtccg gcccttggcg gcggccgacg
     4201 gcccctcgta cttcttcgtg tagcgggcgt tgttccaggg attgcggtcg aggacggtcc
     4261 ggcgctccgg gtcgacctcg gccttcacga gcggcgacga ggacgtgacg cggtacttga
     4321 tccacccgct cgcgccgtcc caggtgcgcg tgacggtcgt cctgtccttg aacgtgagga
     4381 cgaccttcgc gggcatctcg atgtcgccgt cccgcccgac ggtgacgagg gactgccacg
     4441 tgcccttgtc gtccttgtcc ttgtcgtctt tcctcttnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnngcg gaacgccttc cagaacgtct cttctccgag gaggagccgg
     4621 atctgctgca ccgtcgtccc ggtctgcggg tacgagttcc gcccgtacga gccctcggtc
     4681 gcgaacttcc aggaggccga gaggatcggg tcgtggttcg tcgagagggc cgcctgggcg
     4741 cggttgctct cctcgttctt gaacccgatg ccggggggca cggtcaggaa cgctccgtac
     4801 ttgcggtcca ccatcacgat ctccgtgaag gtgttgatcc cctcgtccat ccagctctcc
     4861 tcgaactcgt tcgaggcgac gaggcccatc cagtagccgt gcgtgaactc gtggatcgtg
     4921 accatctccg ggatgtagag acggtcgaac ggccagcgcg ccatccactt gtaggagagc
     4981 gtcgtgtaga tggtctcgta ctccatcccg gtcgcccccg tcccgtcctc gggcgtgtcg
     5041 acgatggaaa tctgcgcgta gggatagggg aacgcccaga ggcccgtcca cgcgagcgcc
     5101 cacttctgcg cctcgatgtg gcggcggtcg aaggcgccgt gctcgggctg gcggtagagc
     5161 cggatcgcga ccttccggaa cccggcgagc aggtcggcct cggtgcggcc gagcgtcttc
     5221 gcggcgaggg cgagctcgtc cttcggaatg tccttcgcgg ggtcgaagac atcctcgacg
     5281 acgacggtgc gcgggtcggc cgtgaaggcg aagttgtgga cggccttctg ctcgtagacg
     5341 agggtcttct tcccgccctc cctcttctcg gagacgaggg cgcccgccga gccgacgacg
     5401 aacttctcgg gcagcgtgat cgcgacgcgc cagtcgcccc agtccgcgta gaactccgag
     5461 ttcgcgtggt actggtggca gttccagccg gcctcggccc ggcggcgccg tcccttcggc
     5521 tcgtagacgc cgatctgcgg gaaccactgc ccgaagaaga agaagtcacg gacgtagccg
     5581 gcgcgcgcat agaccttcgg gaccttcgcc ttccacgcga tcgcgagcgt cgcggtctcg
     5641 ccgggagcga cggggcgggg gagggggacg acgaggacgg tccggtcgtc ggggttgccg
     5701 tcgtccgggc tctcgaagcg cgcgcccttc atcagctcgg cgccgtccca cttcatggac
     5761 gtcacgtccg tgtaacccca gcccttgccg gtgtcggcct tgtcgccgcg cagctggccg
     5821 ccggactccc tgaagaacgt cgagcggtcg ttccggaacg cgttccagta gaggtgaaag
     5881 cgcagctccc cgacggggcc atcggacggg ttcgtccact tcagggtctc ggagccctcg
     5941 acgagcttcg tctcggggtc gagggacacc ttgatgtcgt agtcgacgat cttccgcggc
     6001 gcctcgagcg cggatgcggg cagggcgagc gcgagcgacg cggcggcggc cagtgcgagt
     6061 tgtctcatgg tcgcggggga ttctagccct gcgatagagt tccccgatgc gcgcgggcga
     6121 cctcgtctct ctcaccggcg gcacgggctt cgtcggaagc cacgtcgccg acgcgctcct
     6181 cgccgccggg taccgcgtgc gcgcgctcgt gcgccgcccg gacgacgtcg cgtggctgaa
     6241 gggcaccggc gtcgacctcg tgaagggcga cgtccgcgac gcggcgacgc tgccggccct
     6301 cgtcgagggc gcgtcggccg tcgtccacgt cgcggggaag acctcggcgc ggaacgaggc
     6361 cgagtacatg gccgccaatg cggcgggcac ggagaacgtc gcggccgcct cccggcggca
     6421 cgcgcccggc gcccacgtcg tcctcgtctc gtcgcaggcg gccggcggcc cgagcgtggg
     6481 cggcgtgccc gtgaaggccg gagatcagcc gcgtcccgtc tcggcctacg gccggtcgaa
     6541 gctcgcgggg gaagaggcgc tccgccgctc gggcgtcccg ttcacgatcc tccgcccgag
     6601 cggcgtctac ggcccgcggg agaccgccat ccgcgacctg ttcgtcgcgg cgtcgaaggg
     6661 cgtcgtcccg atcctggccg gcgggcggcc gcggatccag ctcgcgtacg ggcccgacgt
     6721 ggcggcagcc gtcgcgggcg ccctcgagag agggccgcgc ggggagacgt tctacgtcgc
     6781 ccatcccgag gtcctggatt acggttcgat cgccgagacc ctttccaggc tgccctcccg
     6841 gaagccctgg cggctgcccg tcccgtccgg cgtggtccgc ggagccggcc acctcgcggg
     6901 ggcggtgtcg cggttcggaa agggcccccc ggtcttcaac gcagagaagg cgaacgagat
     6961 gctgcaggag gcctggctgt gcgacgtctc cgacgcacaa gccgctctcg gacaaccatt
     7021 tagaactgat tttgcgaccg ggtcgcggct gacctgggac tggtatctcg agcggggttg
     7081 gctcgtggac cggggtgata aaattccctt gagggagacc agatgacaca gccacgttcg
     7141 gcgcggcccg gggccgcaga gggtgaaacg atgtccgcga ggacgcacgg cggatccgcc
     7201 gcgtccgcga accgttcaat cttcgaaaaa tgccggacgt acaccgctcc ggacgacctc
     7261 cgggcggcgg gcctcttcac gttcttccgc atgatcgagt ccggccagga ccccgtcgtg
     7321 atgatgggcg gccgcgagat ggtcatgctc gggtcgaaca actacctcgg cctgacctcg
     7381 caccccaaga tcaaggaagc cgcgatcgcc gccgtgcgga agtacggcgc gggctgcgcc
     7441 ggcagccgtc tcctgaacgg cacgctcgac atccacgtcc agctcgagga gcgcctcgcc
     7501 gcgttcatga agaagcccgc gtgcgtgacg ttctcgacgg ggttcctcgt gaacctcggc
     7561 gtcctgtcgt ccctcgcgag caagggcgac acgatctacc tggaccgcca ggaccatgcc
     7621 tgcatcttcg acggggcgcg cctggccgtc ggcgcggaca tcaagaagtt caagcacaac
     7681 gacccggccg acctccggcg gctcctcgcg atcggcgggg accgcagcgg cggccggatg
     7741 ctcgtcatcg acggcgtctt ctccatggag ggcgacatcg ccccgctgcc cgagtacgcc
     7801 gacatctgcg acgactacga catggccctc atggtggacg acgcgcacgg cgtcggcgtc
     7861 ctcggcaagc atggccgcgg cacggcggag cacttcggca tcgaggaccg gaccgacatc
     7921 atcatgggga cgttctcgaa gtccctcgcg acggtcggcg gattcgtcgt cggcgagccg
     7981 gacgtgatca agtacgtcaa gcaccgcgcg cgggcgctca tgttcacggc agcgccgccg
     8041 cccgcgtccg tcgcggccgt cctcgccgcg ctggacatca tcgagagcga gcccgagcgg
     8101 cgggaccggc tgtgggagaa cacgcgcttc atgctcacgg cgctccgggc gatcggcttc
     8161 gactgcggcg agtcctcgac cccggtcatc cccgtcgtcg tcggtccgga cgaggtcgcg
     8221 ttcaagatgg ccaagatgct cgagaacgag ggcgtcttcg tgaacgccgt cgtgagcccc
     8281 gcgacgcccc cggggcgcgc gctcatccgc acgtcctaca tggcgacgca cacgaaggcg
     8341 cacctcacgc gcgccgtcga ggcgttccac aaggtcggcc gcgagctcgg gctcgtttcg
     8401 taagttcgcg cctcatccgg ccctgcgtct ctcagatgag gccgatgttc acggtgttgg
     8461 tgccgggcct caggaccacc gactggtcct ggctcgagtc gcccccgtag cggacggtta
     8521 cggtccacgt gccggaaacg agcccaccga gcaagtagct gccgtcggat gccgtcgtcg
     8581 tcgacttccc ctggcactcg acgatcgcgc ccgcgacggg tggggttccc gcgtaggcgc
     8641 tgacgatgcc cgccatgatg gccggttctc ccgcgacgac cgtcggcgcg ggtgtgggcg
     8701 tcttcgccac gaaggccacg ggctccgtgg ggctcttcgc ctcgttgcag gccgttgcga
     8761 ggaagcccgc gagcgcgacg gcgatcggag caaaaagagg ttggggagaa cgtctaatca
     8821 tggtgctgct cacgggtttc ctcagaaggg aagcgtgacg tcacgatgcc cgacatccag
     8881 gctgccggag gcggagcgcc gacgacgaag ttgaccgtgt tggccccggg gcggagcgtg
     8941 acggtctcgt attcctcgtc gttgtagccg ttccaggtca ggctgacgtt ccatcgtccc
     9001 gcgtagagcc cggtcagggc gtaggagccg tccgcgccga tgttcacgcc cttcctctgg
     9061 cacgaaagga tcgcactcgg ggccggctcg cctgaaggca gccggacgaa gccgctcatc
     9121 gaagcgggct cgccggtcac gatattgatg gcgaggtcct tgacgttgtc gccgacggca
     9181 agtaccacgt cgaagtcctg ggacttctgc tgcccctgcg cgtaaacctt gacgacggct
     9241 gcccccgcct tcagcccggt gaggcggtac ttcccgcgcg ggtccgcgct cacttcggtg
     9301 cttcccaccc cctggcagta cacgacagcc ggagagtagg gagcgcccga ctgatccgtg
     9361 acgactcccg acagggtgac cgcgggtccg gccggcgtgg gcgtcgcggc gacgacggcc
     9421 gtgggctcgg tcggcgtctt cgtctcggtg caggcgatgc caaggagtgc gaaggtcaat
     9481 gggaggtagc gagcgcagaa ccggcccgtc gctgggttca tcgtgaacct ccgttcttcg
     9541 atgctcggga tcagtacgga atgttgaagt tctgcgtgtt cgcaccaggg ccgagcgtca
     9601 cgttcgtata gaactcgccc aggtagacgt cccacccgat gagaatctcc caggtccctg
     9661 actcgagtcc cgccagcgcg taggacccgt cggcagcgac gggagcggac cgtccctggc
     9721 aatagagggt gatccctgcg atcgggcggg agctctgcgg aatgatccgg cccgagaggc
     9781 tcgccggctg gccgtggacg acggtaatcg agacgttctt tgtgttcgtt cccgggacga
     9841 gctcgacctc gaaggaagtt ggaatcgtct gtcccggcgg atagacgtcg agcgtggtct
     9901 tgcctgccct gagataggtg agggtaaagg tcccgaagga ggggccgccg gggccccacg
     9961 tggtcactcg ctggccctgg catatgacga cgattccgcc ttgtggctcg cccgcctggt
    10021 ctgtgaccct tcccgagagg aagacgctgc tggaaggggc cggcgtcatc ggctcggtcg
    10081 gcgtcttcgt ctcgctgcac gcggcgccga gaaaagtcga ggcggcaacg acccatgtga
    10141 gtactttgcg tcccatcgtg agtcctttca ttcgcagagg aagaagtcct gggtgtggcg
    10201 gagtcacggc ggtctcctca ctcggcgccc gtctcggtct tgtagacgcc gtgcgggccg
    10261 gcggcgtaga ggacgttcgg gtctttcggg tccagggtca ggccgtagac gttcaggtcg
    10321 ccgagtccgg cgttcacgga aaaccagctc gctcccgcgt cgcggctctt gaagacgccg
    10381 ccgccttccg tggcggcgta gacgacggac gggttcgacg gatcgacggc gacgacgccc
    10441 gagcggctca tgcgcgtgaa ggtcatcccc gagctgcgcg gcgaaaacgt cagccctccg
    10501 tccgtgctgc gtgagacgcc ggccgtctgc gccgcggccc agatcacgtc gggatccaac
    10561 gggtcgaccg tgagggacag cgtgaacgtc gtcgacgagc gcttgaacga agcgccgccg
    10621 tccgtcgagc gccagatgcc ggtgttcccg ccgacgagga ccctgcccgg ccgcgcagca
    10681 tcgaccgcga tcgcacggac ctgcggcatc gtgagggagg tcacgcgcgt gaagtgggcg
    10741 ccgtcgtcga cgctgcgcca caggccctca ccccgcgtcc ccgcccagat tgtccccgtg
    10801 agcgggtcga cggcggagat ccagacgtac acgcccggcg agccgaaaac gcggcgcgtc
    10861 cagtttgcgc cgccgtcgtc gctcacgtag agcccgccgc cgttgaagga tccgacgagg
    10921 atgcgagcgg gtttccgcgg gtcgaagacg gcccttcgcg aggcacgact gagaaatcca
    10981 cggcccgcct gcgaccacga ccgcccgccg tcccgcgagt cgaggaggcc accgtcgttg
    11041 gtgctgagca tgagccggct cgagtcgcgg gggtcgacgg acagcgacac ggcgttctgc
    11101 gcgtttatcg ccgagagcgc gtcgaggccg gcgcggcgca ggcgataccg ccggcgccgg
    11161 gcggggaggc cggggacgtc cgcgtcccct cccgaggtcg tgaggacgcc gtcggctccc
    11221 gtaccggccc agacgtcgcc gtccgcggtg acggtcagcg agaggacgtt gcccgaccgg
    11281 acgctcgtcg gccgccaggt cacacccccg tcttcggtca cggcgagtcc gccggagatc
    11341 gtgccggccc agacgcgatc cggggcggtc gggtccacgg cgagggcgag gacgacgccg
    11401 gcatctggag agccgacacg tgtaaacgtc gcgccgccat cggtgctctt gaagacgccg
    11461 tcgccgttca cgccggcgta gacgacgtcg ggccgggtgg ggtcgaccgc aatcgcccag
    11521 acgaccggtt gaaccgccag ggcggcccac gtcgtgccgc cgtcgacgct cttgaaggct
    11581 cccgagtcgc cgcttgccgc gtagaccacg ccggcgcggt gcgggtcggg cgcgatggac
    11641 cagatggtcg tcggcgggat tccggcgtcg gacttgaccc acgtggcgcc gcggtcggtc
    11701 gacttcgaga gtgacgcgga gctgcccacg ccgtagccgg cgtaccagac acgcgggtcg
    11761 agagggtcga ccgcgacgga gacgacgttc gtgtccacgt cggaggtcgg tgaccaggtg
    11821 cggcctccat cgctcgtgcg gaagatcccg ccgcctgccg aggccacgac gacgacgtcc
    11881 gggtcggtcg ggtccatcgc gagcgacgtc acgccgagag cgaggtcgct gagtccgcgg
    11941 tttgcggccg cgaacgtggc gccgccgtcg aggctcttca aaatgccgcc tccgaacgtg
    12001 ccgacgtaga cggtgccgga ggcggggtgg accgcgaccg cgccttcgat cgcgggaatc
    12061 ggagggccga gagcggtcca cgcggccgcg gccgttcggg ccgtaaggaa agcgagggcg
    12121 gccagcgggg cccgccttgc ggctgagaat cggtcgcgaa tcctcatgcg ccggaatgtg
    12181 cggcaccgcc gggcccgagt cttgagccga ttctgagttg tttctcagga tgcggccgcg
    12241 cctccttgcg gcggccgaat gcctccataa ttgagactca cacgcacgag ggaggacgcg
    12301 tgccgccgct cgacgcgggc tcgaaggtcg gaccgtacgt cgtcgtcgcc cggctcggga
    12361 ctggcggcat gggcgaggtc tggcgcgccc gcgacgagcg gctcgaccgc gaggtcgccc
    12421 tgaaggtcct gccgtccggg ttcctcgagg acgaggagcg ccggctccgc ttcgagcgcg
    12481 aggcgaagct cctcgccagc ctgagccacc cgaacgtcgc gacgctctac gcgttcgagg
    12541 agatcccggc ctcggccgat tcgccgcggc gccacgtcct cgccatggag ctcatcgagg
    12601 gagggacgct gcgccgactt ctcgacgcag gccgcccgcc gctccggcgc gcgctccgga
    12661 tcgcggccgc gatcgcggac ggcctcgccg cgacgcacgc gaaagggatc gtccaccgcg
    12721 acctcaagcc ggagaacgtc gccgtgacgg acgacgacat cgtgaaggtc ctcgacttcg
    12781 ggatcgcgaa gtcctctccg ctcgccgcgg ccgggcgcga cgacaccctg ccggaggcgg
    12841 cgctgggaac ggagccgggg tcgttcctcg ggacggtcgg gtacatgtcg cccgaacagg
    12901 cggccgggga aagcgtcgac tatcggtccg accaattcgc gttcgggacg atcctttacg
    12961 agctcgcgac cggcgaacgg gcgtggacga agccgaccgc cgccgagacg ctcgtcgcga
    13021 tcctgcgcga ggagcctccg aggacgggtg cgttacccgc cgcgctccgg aggatcgtcg
    13081 agcgatgcct cgcgaaggat cccgcggcgc gcttcgcgtc tacgcgcgac ctcgcccgcg
    13141 acgtgcgcga cctgctcgag ggcacggacg ccgtgcccga ggtgaccgtc gcgccgcgcg
    13201 tgcgctctcg tacccggctc ccgcggttcg cgtgggcggc cgccctcgcc gcgtcggccg
    13261 tcgtcgcctt cttcctcgtg cgcgggcgcc tcgaaacgaa accggtgtcc tcgatcgccg
    13321 tgctgccctt cgagggcggc ccgggcggcg agaccgagga ttacgtgcgc gacggcctga
    13381 cagagaccct gatcgagcgg ctgtcacgtc tcccggacgt gcgcgtcatg gcgcgggcga
    13441 cggtgttccg gttcaggggc tcgacggacc cagtgaaggc cggaaaggcg ctcggtgtgt
    13501 cggcggtcgt caccgggagc gtggcgcggc ggggggatcg catcctggtg tcggccgagg
    13561 cggtcgatgc gcgcacgggt gcccgtctct ggggagaccg gtacgaccgg cccgtcgtgg
    13621 acctcgtgcg ggtggaggcg gaactcgcgt cggacctcgc agcgggtctc aggctccgcg
    13681 cgtccgagcc cgagcggcgc gcgctcgcga ggccgggcac gtcgagctcg gaggcgtacg
    13741 acgcctacct gcgcgcgagg ttcgcctcgc tccaggacac cgaggccggc tacctcgagg
    13801 cgatccgtct cttccgcgcg gcgacggaga aggacccgtc cttcgcggag gcgcatctcc
    13861 gtgcggcgga cacgtacggc gagatggcgt cgggcggcta ccttccgccg gcggaggcgt
    13921 gggcccgctc ggacgaaggc gcccgccgcg ccctcgtcct cgacccggac ctcccggacg
    13981 cgaaggcggc cctggcggcc cgccgtgcgt actacgactg ggacttcgac ggcgccgagc
    14041 gcgacttccg cgagctgttc gacggtccgc ggcctccggt ctggcacgcc cgcgcgttcg
    14101 cgctcctcct ctgggcccga gggcggaccg aggaggcgat cgagcggacg gagcgggcgc
    14161 ggacgctcga cccgggcaac gtggtcttca cggtggcttc gggcgactac cgggcgcagg
    14221 cgggacgtct cggcgaggcg gtcggtctct acagggcggc aatcgacgcc gagcccgagg
    14281 acccgcgtgc gtggttcggg ctcgcggcga tcttccggag ccgcggagag gcggcccgcg
    14341 cgatcgagag tctgcgcaag gcgtacgcgt tcgccggcga gaaggacggc gcggaggctc
    14401 tcgcgggcgc gtccaccgtg gcggactacg acgcggtgca gctgcgcgtc gcccgctcgc
    14461 gactcgccga agccgaggcg ctggcgcgca cgcggtacgt atcgccgctc gacacggccc
    14521 gactcgcggc gatggcgggg gagaaggagc gcgcgctcgc gggtctcggc gcggcgctga
    14581 aggagcgctc gccgggcctc gtcttcctga acgtcgatcg tgtgtgggac gtcgtccgcg
    14641 cagatccgcg cttctcggcg gtcgtgcgca aggtggggtt gtcgtgagct gtgggggggg
    14701 cacgggcgtc gcctccgcgg cgaggcggtg agggaggcaa tggccacgac gacctcgtcc
    14761 cggcgggtgc ccctgggagc ggtcgccttc gacccgccac gccgggtcct cgtcgaccgt
    14821 ggcgccgtcg tcgcgctctc gcgcctcgag tacgctcttc tctcacttct cgtcgagcgc
    14881 cgaccacggg ccgtttcgaa ggacgagatc caggagatcc tttggcccga cgtcgccgtg
    14941 tcggaaacga gcctcacgac actcgtgaag gacttgcggg ccaagctcag acagtcgggg
    15001 cgcgaggggc cgatccgtac ggtgcacggc tacggttatg ccctcgaggg cgcgcccggc
    15061 gccgggcccg cgccgccgcg cctggtgcgg ggccggacgg agatcccggc cgtgtccccg
    15121 gatctggtcc tcggccgaga gccggggaaa ccgggctcga tcgacgacgc gtccgtctcg
    15181 cgccgccacg cccgcctcac gtggaacgcg tccgggctcg ttctcgagga tctcggcagc
    15241 aagaatggga cgtacgtgcg cggcgagcgc atcacgggcc cggtggcgct gaaggacggc
    15301 gacgacgtgc gcctcggcct cgtgacgttc gtcttccggg cggcgccgac cggcgcgtcg
    15361 acgacaaaga ccgtggcata gttctccccg tgaacgccat tcagatcgac cgcgtcgatt
    15421 cctcgcgtgc cctgcgcgac ttcgtggacc ttccgttccg catctacgcg gacgacaagg
    15481 actgggccgc gccgctcaag tccgacgtcc ggtggatgct cgacctcgcg aagaacccgt
    15541 tctggaagca cgcgaagcgg gagttgttcc tcgcgcggcg ggacgggaag gtcctcgggc
    15601 gcatcgccgc gatcgtggac gacgagcaca accgggtcca cggcgagaag atcggattct
    15661 tcggattctt cgagagtgaa gacgatcggg aagtggcggc ggccctgttc cgcgccgccg
    15721 aagcgtgggt gcgcggcgtt cttccggagg cctcgctcct gcggggaccc gtcaatccct
    15781 cgctgaacga cgaggccggt gcgctcgtgc cctccgagag cgagccgggc ctgcctttca
    15841 tcatgatgac gtacaacccg gcgtactacc tcgacctctt cggggccgcc ggttacgcga
    15901 aggtgaagga cctcgtcgcg atcctcgcgc cggtggacga ccgttcgttc gagcgcctca
    15961 ggcgcgtgac ggacatcgtg aagaagcgcg agaagggacg ggccgtcgtc cgccagatcc
    16021 ggatggacaa gttcgacgag gacctcgcga tcgtgaagga cctctacaac cggtcgtggg
    16081 agaagaactg gggcttcgtg ccgatgacgt ccgacgagat cgacgccatg gcgaagaagc
    16141 tcaagccgat cctgtacccg ccgtgggtgc tcttcgtcga gctggacggc aaaccggtcg
    16201 gcttccacct cgccctcaag gactacaacc aggtcctcaa ggagctgggc ggctcgctct
    16261 tcccgttcgg gtggctgaag ttcctgctcc tgcagaagaa gatcgaccgc gtgcggacgc
    16321 tcgcgctcgg catcctgccc gagtaccgcc gccgcggctt cgacgcgctg ctctacttcg
    16381 aggccgtcaa agaaggcgtg cgcctcaagt acacggctgc cgagttctcg tggctgctcg
    16441 aggacaacct cgacatccag cgcccgctcg aggtgttcgg cggccggatc taccgccgct
    16501 acaggatcgt cgagcggagc ctcgcctgag cggacgcacg gtcgccgcgt aagatcacat
    16561 ccagcttttt catggcgctc cagcccggag accggctcgg tccgttcgag gtcgaggttc
    16621 tcctcggagt cggggggatg ggcgaggtct atcgcggccg cgacacgcgg ctgggtcgcg
    16681 acgtcgcgct gaaagtgatc tctccgaagc gcgtcgggga cgcggacctg agacgccgct
    16741 tcgagctgga ggctcgcgcg gcgtccgtcc tgaaccaccc gtcgatcgtc acgatctacg
    16801 acgtgggaga gacgaacggc gtttcctgga tcgcgatgga gtgggtcgag ggacggaccc
    16861 tgcgcgaggt gctcgccgac ggcccgcttc cgatgcggga cgtcctcacg atcggcaggc
    16921 agatcgcgga ggggctctgc gcggcccacg aacggggcat cgtccaccgc gacctgaagc
    16981 cggaaaacgt catggtcggg cgggacgggc gaacgcggat cctcgacttc ggcctcgcgc
    17041 gccagacgtt cgcgggagcg ctctcggggg gcagctcggc gatcgagacc ctcgcgaccc
    17101 ccgcggcggt cacgggggaa ggcgtcattc tcggaacggt gggctacatg tccccggagc
    17161 aggcctcggg gctgccggcg gatgcgcggt cggatcagtt cgccctgggg ctgatcgtgt
    17221 acgagatggt ggcgggccgc cgggccttcg cgcgtgcgac cgcggtggag acgctcacgg
    17281 ccatcatcaa ggaggagccg ccgcctcttt cgaccgttcg tctcgacgtc cctccggcgc
    17341 tggagagcgt gatcgcgcac tgcctcgcga agcgccccga gaagcgattc gagtccacgc
    17401 gggaggttgc ggcggctctc gggacggacg cgacggggag cgcccccatt tccttctcgc
    17461 ccacgctcgc cgtggtcgac gcgcctcccc tcgaacggcg ccggctgccg ctccggcgcg
    17521 cggtcgtcgt catcggcgcc gtggcgctgc ttgcgggtgg cgtggcggcc taccgcgctc
    17581 gcgccgcgaa gggcgcgatc aggtccctgg cgatccttcc gttcgaaaac gcgagcaagg
    17641 accccgaagt cgagtacctc ggggacgggc tcactgacaa cctcatcaac cagatgtccc
    17701 gcgtggcctc cctcaaggtg atggcgcgcg cgaccgtgtt ccgcttcaag ggatcgctcg
    17761 atccgcagga cgccgggaag aagctcgggg tcggcgcggt cctgacgggc accgtcctga
    17821 ggcgcgggga ccggctgtcg atctcggtgg agctcgtgga agtgcctacc ggcgcccggc
    17881 tctggggcga gaaatacgat cgcccctcct ccgatctgat ccgcacgcag gacgacatcg
    17941 cggccgacat ctcggacgga cttcggcttc ggctttcggg cgcggagaag agcgtgctcg
    18001 gccggcacgg cacggagaat tccgaggcct acgatctggc gctcaaggct cgctacttcg
    18061 tcctcaagga cacggaagag ggttacctcg aagggcgtcg cctctatctc ctggccgccg
    18121 agaaggaccc ggcgttcgcc gaggcctggc tcggggtgtc gaacacctac ggcgtgatgg
    18181 ccgtgaacgg gctgctcgcg cccgcggaag cgtgggcgcg ctcggacgag ggggcccgca
    18241 agaccctcga cctcgaccct ggaaacgtgg gggcgcgctg cgccctcgtc acccgacggt
    18301 tcttcttcga ttgggactgg gcgggcaccg agcacgagtt tcgggagctg ggtgcggacc
    18361 cccgggtgat tcgcggagaa ggacttcgcc cgatcgggct ctcgctctgg gcccgcggcc
    18421 gggcggacga ggcgttcgtt ctcatggaga gggccatgcg cctcgacccc ggaaacgtgg
    18481 ccgccatgat cgccacgggt gactacctcg cgcaggccgg gaagctcgcg gaggccgcgg
    18541 acgcctacgg ggcggccatg aaggccgagc cttccgactc acgtccgttg ttcggcctct
    18601 cggacgtcct cctgcgccgc cgcgacgtga ccggcgcgat cgacgcgttg cgcagggcgt
    18661 acgagctcga cggcgaggac gagggcgtcc ggttactcgc ttcggcccgg acggagaatg
    18721 acctcgacac cgcgaagatc ggcgttgcgc ggagccggct cgccgaagtc gaggaaatgt
    18781 cgcggaagag gtacgtcagc cctctcgacg tcgcccggct caacgcgcag gccggcgaac
    18841 gcgagaaggc cttcgcgagc ctcgaggcgg ctttcaagga gaggtccccg ggcctcgtcc
    18901 tgctcagggt ggaccgggcg tgggaccgga tccgcgagga tccgcgcttc acggatctcg
    18961 tccggcgcgt gggcattccc tgacgtgggc tcggcgcacc cgcccgcgtt tgttatgatt
    19021 tcgtaaagac gcggactcag tatcaggctt tacctgcgca ggaggtctcc atgagggctc
    19081 gaagcgtcgc attttccgcc gtggccttcg ccggcctcct ggccggagcc gacctcctcg
    19141 cctgcggcga caagttcctc gtggtcggcc gcggcacgcg ctatcagagg ccgaagggcg
    19201 cccgcgcggc gtcggtcctc ttttacgcga gtgcggagtc cggcctctcc gccatgctca
    19261 aggcgatgcc cgtcgattcc gtcctcaagc gcgaagggca tcggggaacc tcggtgacga
    19321 cgcccgagca gctctccgcc gccctggccg gcggccattt cgacgtgatt ttcgcggacg
    19381 ccgcagacgc gtcgacggtc gagcggcttc tcgcgggacg cgccgacgct cccacgctcc
    19441 tgccgttctg cgacggcacg aagagcacgt ccgttgtcgt cgaccagaag ggcgccttct
    19501 gcgtcacgac tcctccgaag gagcgctcca tgctcgaggc gatcgaccag gcggtcgagc
    19561 gtcgcgatcg aagggtcgca aagccggcga tccgctcctg acggcacgat gcaaggagtg
    19621 cgacggttcc tccctcgagc cgtgctcgcc gccgtcgtcg ccgcggcctc gccgcgcctc
    19681 gacgctcagg ctttcacgct tccccgggga gtggccgcgt tcacgatcgt ctggcagtac
    19741 gtcgacaaca ccggccaccg catgacggac ggatacgtcc tggaccaggg ccagagcctt
    19801 tcgacgggca tcctggccga gctggattac ggcgtcacgg accggttctc cgcgaccgtc
    19861 ggaatcccct acatcttcgc gaagtacacc ggcggcccgc cgccgcccgg gattccgtac
    19921 ctcccggtcg actcctgcaa ctgctggcat tccggattcc aggacttcgc cttcacggcg
    19981 cgctatcgct tcggggacga cccgtgggcg gtcacgccgc tcgcgcgcta cgtcctgccg
    20041 agtcactcct acaactacca gggcgaggcg gtcatcggcc acgatctcca ggagtttcag
    20101 ctcggcgcct tcgcgagcct gaggctcgtg actctgctgc ccgacgcgaa catccaggtc
    20161 gggtacgtgt acagcttcgt cgagaaggtg ctcgggatcc ccaacgaccg gagcaacacg
    20221 tccttcgaga tcggctatct cttcggacgc cggttctacg tccgcgcggc cggctactgg
    20281 caggtcacgc acggaggcct gcgtctcggg tccgttaccg gaaacccgtt cccgatcacg
    20341 ggcgaggtga acactccgga actgggtgcc gagcacgacc ggttgctgcg cgacaactac
    20401 tggcgggtcg gaggaggcgt ttcctattcc gcgggcccgt tcgacgtctt cgcgtcgatc
    20461 tcgaagtacg tcgccggaac ggacacgcac aacggacagg cctacaccgt cggcatgacc
    20521 tggtacttcg gcacccccaa gtagctaccc ggccgccggg tctccgaagc gcctgtagag
    20581 cgcgttcccg agcgggaccg ccacgaacgc gagcgcgagg cccgcgagga cgtccgcgac
    20641 gtagtggtac cggcagaaga ccgtcgcgat cacgaggccc accgcgaaag gcaggaacca
    20701 ccagaacgtc gtccgtgacc ttcgcgcggc ctcgaggagc accaccgtca cgaccatcgt
    20761 gtggccggac gggaagcagt cgcgcttgtt gctctcgagc ttgttcagcg tgtcgtcgat
    20821 gacgcgggcg acggcgccgc gggggagcgg gcccgcgtgc gtcacggtga agcgcgggcc
    20881 cacggccggg acgatgaagt agccgacgta gctcacgaag aagacgaaga ccatcgtgaa
    20941 cgcgtagcgc gaaaaatcgc gcgcggccgg gggacgctcc cggtcgtcga gcaggacgag
    21001 cgtgccgagg acgatgggat ggaagtagta gagggaatag aagaacgtga ggacgtccga
    21061 gaggaacggc gtcgcgaacc gctcgagata gaccgtcggg tcggtcccgg tgagccagcg
    21121 gtcgaacgcg atgagcgccg ggtccttgtc gacggggttc acggcccgga tgagcggccc
    21181 cagcccgtcg aagatcacga cgatgcaggc gatgacgtaa tagtcgagga ggatctgaag
    21241 attctttgag gggttcggat tgcggccgcg caaccatgcg gcgacggcgg ggacgagggc
    21301 gaccagcccc attctcagaa gaactcccgg gctctccagc gcctggtatc tgacgaggcc
    21361 ggccacgacg agcccgacgg cgaacagcga cgtcagggcg tcgacgggct tccagcgcac
    21421 cggcgggttc tagcacacga acctataatc cccaccgtgc gctgcgccgc ggtcctcttc
    21481 ctgtccggcg cgctggccgc ttccgcggcc ccggcgcccc cttctgccgc gggaacggtc
    21541 gtcgtcgcgg acctgaacac cgagatccag gccgtgagcg ccgccttcgt cgcgcgcgtg
    21601 ctcaaggacg ccgagaagaa cgccgccccg ttcgtcgtcc tccggatcga cacgccgggc
    21661 gggcgcatcg actcgacccg cacgatcacg caggcgatcc tcgcctcccc cgtgccggtc
    21721 gtcggctggg tggcgccgcc gggctcgcag gccgcgtcgg cgggcttcat catcctgatg
    21781 tcgtgcgacg tcgccgcgat ggccccgggc acgaacgcgg gcgcggcggc gcccgtgggc
    21841 ggagggggag aggacctccc caagacgctc gcgaagaagg ccgggcagga cgtctcggcg
    21901 ctcctgcgct ccgtgacgga gccgcgcggc cggcccgtgg acgacgccgt aaaagccgtg
    21961 agcgaggcgg cgtcttactc ggagagcgag gcgctcaaac ggaaactgat cgagatcgtc
    22021 gcgcgcgacg agaaggacct cctcgcccag ctcgacggcc ggacggtcaa gcgggtcggc
    22081 aagagcgacg ccgttctgaa gacggcgggc ctcgtgacgg ccacgcggac gatgactcgc
    22141 cgcgagcgca tcctctgcgt cgtcgcgaac ccgggcctcg ccggcatcct tttcctgatc
    22201 ggcctcgtcg ggctctattc cgagctctcc catccggggg gaatcctgcc gggcgtcctc
    22261 ggggccatcg cgctcctcct cgcgctcttc gcgatgtccg tcctgcccgt caacgcggcg
    22321 ggagtcgcgc tgctcctggt cggggcgctg ttcttcttcc tcgaggtgaa gctcgcgtcg
    22381 cacggcgtcc tcgccgtcgg cggggccgcg gcggtggtgt tcggagcgat cctgctcttc
    22441 cccgaccagg ccggcgcgcc gaggggagag ttcgcggtcc tcgtcgcggg cgccgtcgcc
    22501 accgcggcga ttctcgccgt cctctcgttc aaggccctcg cgatgaaaag cctgcccgac
    22561 cgcacggggc tcggcgccct cgtcggccag atcgtctccg cgcgcagccc gatcgcgccc
    22621 gtcggcaagg tgttcgccga cggcgcgctc tgggaagcgc attcctcggt acccgtagcc
    22681 gccggcgacc tcgtcgagat cgtcggcatc gaaggcctcg cgctcgtcgt gaggccggcc
    22741 cgcaaggcgg gccaggagcc cggatgaaca acctcacgtt cctcgtcccg atcgtcttcg
    22801 tggcgatcat cttcatctcg aagtggatca acatcctcaa cgagtacgag cgggccgtca
    22861 cgttctggct cggccgcctc tcgccgcagc ccaaggggcc cggcctcacg ttcatcttct
    22921 ggccgttcga gacgatgatc cggatctcgc tccggacggt cgtcctcgac gtgccgccgc
    22981 aggacgtcat cacgcgcgac aacgtgtccg tgaaggtgaa cgcggtcgtc tacttccacg
    23041 tcatggaccc ctcgaaggcc gtcgtgaagg tcgagaacta tctctatgcg acgtcccagc
    23101 tggcccagac gacgctccgc tcgattctcg gccaggccac gctcgacgag ctcctctcct
    23161 cccgcgacaa gctgaacgag aacctgcagg agattctcga caagcacacg gacccctggg
    23221 gcatcaaggt cacgatggtc gagttgaagg ccgtcgacct cccgatcgag atgcagcgcg
    23281 ccatggccaa gcaggcggag gccgagcgcg agaagcgcgc gaagatcatc cacgcgtcgg
    23341 gcgagttcga agccagcaag cagctgtccg aggccgccga gatcatcggc cgcgagcccg
    23401 cgacgctgca gctccggtac ctgcagacgc tcacggagat cgcggtcgag aaaaactcga
    23461 cgatcatctt cccggttccc atggacctca tcagcgtgtt catgaagaag gacaaggcct
    23521 gatggcgggg ctcggtctcg aagaccgcca cgaggagatg ccggagaggg cggcgcccgt
    23581 ccactaccag atctcgatca ccgggcggca ggccgggttc ttcttcctgc tgctcctcgg
    23641 cgcgctcggg ctctcgttct tcttcgggat gaagaccggg gcggccgcga agaagggacc
    23701 cggcggcgga gccgccgggg tgatcgcttc cgcgtccgac atccccgttc ccacgctcgc
    23761 gccggcggaa agccttcgcg aggcgaagag gcccgacgac acgctcggaa tcaagcccga
    23821 ggagaagagg gcggccgagg ccgcgccgcc tccctcgccg gcgccgaccg ccgctccgac
    23881 ggcggtgccg ccgactccga ccgccgcacc ggtcccgact ccgaagcctg cggcggcggc
    23941 gaaacccgcg ccgaaggggc cgttctacgt gcagatcctc gcgacgaagg atgccgccag
    24001 ggccgacgag ctcgccaagc ggctcaagga cgagggattc aacgccgacg tcgcggtcat
    24061 cgccggcaag cccggatggt tccgcgtcca cgtggggccg ttcaaggacc gcgcgaaggc
    24121 cgaggcgctc gcgaagaaga tcaaggccac ggacaagcag atcaagaacg tcccgctcgt
    24181 ggtgccgtga gccgtccgct tcccgtccgc ggcgagccgg cgctgccgcc gtggatccgc
    24241 gagaagaagc tgcgcctcga cgacctccac gcgatcaagg cgcggatgcg gctcgggaag
    24301 ctctcgaccg tgtgcgagga ggcgcgctgc ccgaaccgca cggagtgctt cgagcggaag
    24361 acggcgacgt tcctgatcgc gggagacgtc tgcacgcgcg cctgcgggtt ctgccacatt
    24421 gcgtcgggga aacccgcgcc gctggacccg gcggagcccg cgagcctcgc cgccgccgcg
    24481 cgggacatgg gcctcgccca cgtcgtcgtg acgtcggtgg accgggacga cctgccggac
    24541 ggaggggcgg cgcactgggc cgcggtggtt cgcgcgctca aggaagacga cggcgcgcgg
    24601 acggtcgagg tcctgactcc cgacttccag ggagtcgcgg cccagatcga ctgcgtcgcc
    24661 gacgcgggac ccgacgtcta caaccacaac gtcgaaaccg tcccgcggct ctacgacgcc
    24721 gttcgcccga agtcggattt ccgccgttcc gtcgacctcc tcgcccgcgt caaggcgcgg
    24781 catcccggga tgacggcgaa atcgggcctc atgctcggcc tcggcgagcg cgacgacgag
    24841 gttttggacg tgttccgcgc gctgcggcag gcgggcgtgg acgtcgtgac ggtggggcaa
    24901 tacctccgcc cgtccgcgtg gaatctcccg gtcgccgagt acgcgacgcc ggagcgcttc
    24961 gcggcgctgg gacgcggcgg gatggagatg gggttcaccg ccgtcttcgc cggtccgttc
    25021 gtgcgctcga gctatcgcgc gggggagttc ctcagaaggt gacgaacgtc ttggaggcgc
    25081 gccacgcgcg gcgccggccc tggatgcgcc cgccaccctt caggaagatc ttctcctact
    25141 tccttgccgg cgtcggtgtc ggcgtcgagg cggggcgcga cttcgcggcc tgttcgagcg
    25201 ccgttgcctt ctgcttgcga agcttcgaga tctcggcgtc cttctgcttt tccagtttct
    25261 cgatctcctg atcgagcagc ttgatctggc tcttgagcgc cttgatcttc tgcttcaggg
    25321 attcgagcgc ctggttgatc tgggccagct tggccgcgtt ccccgcgtcg gccttcgcct
    25381 gcgccttcag cgtttcgacc tgcttttcga gctccttgat ctggttctcg agggacgact
    25441 tctcggacct cgcttcctgg atgagcttcc agtactcgcg ctcgatggcc ttctccatcg
    25501 cgtcgtaggc cgcctgaacg tcggagatcg tggaccgcgc cgacagcgcc ggcgttggca
    25561 cgggcctgat ggtctgcggg cgttccgcgc cgaagatcag cgacgacggc acgagcgagg
    25621 ccgccgcgag gagggcgacc gggaacaaag ccgcgagggt gcgcgccggg cgcttcatgg
    25681 agccctattc tccgcccagc cgattcgaac cgctagactg gcgcccgccg taccaggaga
    25741 cgcaccaacg cctcatgctg ccctcattac cctctaagaa attcccccgt cttctcttct
    25801 ccctcgcctc gggcgtcctt ctggctcttt gcttcccgaa cttctcgatt ctgccgctcc
    25861 tccccgtcgc gttgattccg ctcattgcgg ctctctcggg cctgacctgg cgcaaggcat
    25921 ttctgcccgg gttcgtgttc ggagcggcct tctggctcgt cacgatcccg tggatcgcgt
    25981 acacggtgaa ccggttcggc ggcgtcgggc ccgtgctcgc gggactcgcg ctcgcgatca
    26041 cggccaccat ttacgccgtc ccgttcggac tcatggcggc gctctacgcg gcggcccggc
    26101 cgcgcaccgg gccgggaatc gtcgcggcgt ttgcctcggc ctgggtggcc caggagggat
    26161 tccgcacgta catctacgtc ttcggcggct tcccgtggaa cctcctcgcg aacccgctcg
    26221 cggacgtgcc ggcgctgctc ggctcgaccg ccctcggcgg cgtcctcttc acgtccttcc
    26281 tcctcgcggc cctcaacgcc gctctcttcg tcgcgtggac gcgcccgggg cgaaccgagc
    26341 gcctcggctg gctcctcggc gccgccgtgt gcgctctcgc cgcggcggcg gttcgggccc
    26401 cgcgtccccg tccctcggac gaggtcctca aggtcggagt cgtccagccg aacgtcgaac
    26461 aggacatccg ctgggaccgc ggcacggccg agcgcatcca gaccgacctc gaggaccaga
    26521 cgcgcgccct gtgccgggcc gaccggccgg tcctcgtcct ctggccggag agcgcgtcgc
    26581 cttactcgtg gtcgttcaac gccctctatc gcgagagggt cctcaggctc tgccgcgagc
    26641 tcgacgtcgc gatcctgatg agcacggcct ggtcggacgc cccgtgggcc gaggacgcgc
    26701 cgtactacaa cgcggcgctc ctcgtcacga aggacgggcc cgtcctcccg ccgtatttca
    26761 agcagcgcct cgtcccgttc ggggagtacg tgccgctcgc gccgctcctc cggatgatca
    26821 agccgatcag ccgcgcggtg cccggcggct tcacaccggg atcggggacg gtcctcctga
    26881 agctcgggag ctggaatctc ggcggcgccg tctgctacga ggtcgtgtac ccgtggatcg
    26941 cgcgggccga ggcccgcgcg ggagccgacg tcctcttcac gctcacgaac gacgcgtggt
    27001 acgggcgggc gggcgcccag cggcagcact ggcaggct
//
LOCUS       DAOMFY010000023        26746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_590_length_26746_cov_9.255404, whole genome shotgun sequence.
ACCESSION   DAOMFY010000023 DAOMFY010000000
VERSION     DAOMFY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 26746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26746
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_590_length_26746_cov_9.255404"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLB01_03685"
     CDS             <1..367
                     /locus_tag="PLB01_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76433.1"
                     /translation="TFWAYIWFCQYLLIWYSNLPEETPYYALRFQGGWTALFWLNPVV
                     SFLVPFVILLGSRAKKSPAALGQAALVVVLGRWLDAYVMVAPAVGPLPSFPAHAVAAS
                     LLVLAGMALAFDRLHLGNL"
     gene            392..1084
                     /locus_tag="PLB01_03690"
     CDS             392..1084
                     /locus_tag="PLB01_03690"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQQ76434.1"
                     /translation="MQADPNPLALALLDDETVIRRVQQGETALFEIVMRRYNQRLYRI
                     IRSILGSDTDVEDVLQEAYLKAYGHLGQFEGRSSFATWLTKIAVYEASARARKGRRFQ
                     PLEVPVGDDENRVAELRSSLPDPEQDASRHELKRLIEEAVDHLPDIFREVFVMREIEQ
                     MSQAETAECLGLKEETVKTRLHRARRQIESHLLARAGQGIRTAFSFGDSRCTRIVTNV
                     LQRLAVGRIPRA"
     gene            1167..1592
                     /locus_tag="PLB01_03695"
     CDS             1167..1592
                     /locus_tag="PLB01_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQQ76435.1"
                     /translation="MDTRLVSAFWTLRIAFGLTAFLAGLDKFLNLLTNWEQYVSPLIL
                     KVVPLSAGALMRVAGVIEIAAGIAILAGLTRLGSYVVAAWLALIALSLVTGGRYFDVA
                     VRDLVMACGSFALARLSEVREHAAAPSAARAGALSASSV"
     gene            1598..2188
                     /gene="folE"
                     /locus_tag="PLB01_03700"
     CDS             1598..2188
                     /gene="folE"
                     /locus_tag="PLB01_03700"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQQ76436.1"
                     /translation="MRRDLRLAGEPERHDEGEDAVVSLLRMIGEDPSRDGLLETPRRV
                     VQALREMTAGYGEEPSEILSKTFEESSDELIVLRGIDFHSTCEHHLLPFYGTASVGYL
                     PGLVVGISKLARLVHCFARRLQIQERLTRQIADAVAEHLDARGTAVVVKAHHLCMGCR
                     GVKQPGTEMITSAMLGTLREDAVTRGEFLRLAGVGT"
     gene            2381..3640
                     /locus_tag="PLB01_03705"
     CDS             2381..3640
                     /locus_tag="PLB01_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76437.1"
                     /translation="MRSRESADDDDRSTMASSHRMLDEGRRIFRYDTFGDEAFWGDTL
                     RLHEAIAGSANGGVGPGVSPKTALAVGLKVDSDALPPSVVQGIRSGKINLDDPATTLA
                     LLQLDAVVGVTGRFSPGKQIVSLGIQCAFCHSTVDDSLAPGIGRRLDGWPNRDLNVGA
                     IVALSPDLSAVNRLLGVDDATTRTVLASWGPGKFDAELFLDGKVFRPDGRSAATLIPA
                     AFGLTGVNLHTYTGWGSIPYWNAFVAVLEMHGKGNFFDPRLDDPVQFPVAARARSGHA
                     QVAEGEDQVTAKLPALHYYQLSIPAPTPRVGSFDAAAAGRGGVLFAGKGQCASCHTEP
                     LTTEPGWNMHTPEEIGIDDFQSSRSPDKRYRTTPLHGLFTRAKGGFYHDGRFPTLLDV
                     VNHYDSFKGLSLTPAEKRDLVEYLKSL"
     gene            3651..4820
                     /locus_tag="PLB01_03710"
     CDS             3651..4820
                     /locus_tag="PLB01_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ76438.1"
                     /translation="MPDFKYVLVGGGMTAHAAALGIRSVDASGSILLVGDEPEGPYAR
                     PPLTKGLWTGQDEASIWLPAVPGVPVRAGARVVAVDRAARRVVLDGGETIGYERLLLA
                     TGGTPRRLPFGGDDVVYYRTVADYRRVRALPPGRHVAVVGGGFIGSEIAASLSTAGTR
                     VTLVFPEEGIGARLFPRDLSLYLNDFYRQRGVEVRPGERLTGLEAHGPGFTVATDRGR
                     IQADLVVAGLGIVPNDRLAAEAGLDVDDGILVDESLRTSDPAVFAAGDVARFWSPALG
                     RRIRVEHEDNANSMGREAGRAMAGADVAYRHLPFFYSDLFDLGYEAVGVLDPRLETVA
                     DWKDPFRKGFVYTLDDGRVRGMLAWGVFGKMDEARSLIAEPGPHAPESLRGRISA"
     gene            4836..5297
                     /locus_tag="PLB01_03715"
     CDS             4836..5297
                     /locus_tag="PLB01_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQQ76439.1"
                     /translation="MSDKARIDAHVTTPEAYETLLALEDYLDRCGLERSLIELVKLRA
                     SQINGCAYCIDMHTKDARAAGESEQRLYLLDAWRESPFYSSRERAALGWTESVTRVSE
                     THVPDEVYDEARRHFSEKELVDLNWVVVAINAWNRLAIPFRTPPGIYQPAP"
     gene            complement(5332..5778)
                     /locus_tag="PLB01_03720"
     CDS             complement(5332..5778)
                     /locus_tag="PLB01_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQQ76440.1"
                     /translation="MTALPDDLPRRVLDGSPDAVLISDPTGTVRYWNAAAERVFGFRA
                     AEAVGISMDFIIPERLRGRHWAGWEATMKTGVTRYGEGRMLAVPALHKDGRQISVEFS
                     IQLLKDAGDRIQWVVAVFRDVTERYAREKALRAQLKSLEAKAAGQV"
     gene            complement(5798..6472)
                     /locus_tag="PLB01_03725"
     CDS             complement(5798..6472)
                     /locus_tag="PLB01_03725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ76441.1"
                     /translation="MSGRWEDAGVVQGFSKAAANQVLLAFVRLELARRPGLRVLDLGC
                     GAARNAVPMAAEGAAVVGTDIAWPMLAAARRRVASEGVAGRVTLARAPMDRLPLPDAS
                     VDLVVAHGIWNLARSASEMRRAIAEAGRVARPGAGLFLFTFSRATLPPDDRPVPGEPF
                     VFTRFAGEPQCFLTEAEVLAELARGGFEKDPPGPLTEYNRPPAGAIARGGPVIYEGTF
                     RAARQS"
     gene            complement(6475..7203)
                     /gene="ric"
                     /locus_tag="PLB01_03730"
     CDS             complement(6475..7203)
                     /gene="ric"
                     /locus_tag="PLB01_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HQQ76442.1"
                     /translation="MRITAETFVADIAAHHPRSIEVFERHGIDFCCGGRRPLGEACRE
                     HGAHVEAVAAEIEAAAAREVPEDRIFTDAPLATLLDHIVSRYHGALREDLPLLGRLAD
                     KVAEVHGMRHPELVELARVYRELRSELEPHLATEEDRVFPSVRLLAESGAPAENVKGA
                     LHALEEEHDRAGAALASLRALSRGFVVPDDACATYQALYEGLVRFERELHEHVHLENN
                     VLFPRVVSLEMSAAASRAPTEPVR"
     gene            7343..9649
                     /locus_tag="PLB01_03735"
     CDS             7343..9649
                     /locus_tag="PLB01_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HQQ76443.1"
                     /translation="MSEHERLSPWWRHGAILAMIFGFSILSIVTVQTYKNAPPIPDRV
                     VDESGRTIFTGADVLAGQDVFLKYGLMEHGTLWGHGAYLGPDYTAETLHRLAEVWRDT
                     LAKSRYQSAYAGLDAGRQAAIDAEVRTAARTNTWDPAAKTITFPPGAAAAFAVAKTEW
                     AAYFGGETAAPGLPARYIKNPKELDALSAYFSWATWATVASRPGKDYSYTNNFPYEPL
                     VGNSPTSATYLWSGLSLIALLGALGTILFVFGRFDFLGWKAESGALHHHDPPRALTLT
                     PSQRVVGWFLAVVAVLFLLQTLAGGALAHYRVEPGAFYGFDLASILPYTLLRTWHLQL
                     AIFWIATAWVAGGLFLAPLLGGGDPKGQKAGALVLLGALAVVVFGSLFGEFLGLRNAL
                     GRFWFWFGHQGSEYLDLGRFWQILLAAGLVLWLVLMFRALRPAMKSPTTGTLASLFLY
                     IAVAIPLFYLPALVYGPHTNPAVIDNWRFWIIHLWVEGFFELFATVLVAVMFFQMGLV
                     STKTATRVVYLDAILYLGAGIVGTGHHWYFTGQTTLNMGLAATFSAMEVVPLTLLTLD
                     AWDFIRLKDRTCGSCGVTLSAAQKWAIHFLMAVGVWNFVGAGVFGFLINLPVVSYFEV
                     GTTLTSNHGHAAMFGVFGMLALAVVVYCLRALQSEVRWARVEGFVKTGFWGLNVGLAL
                     MVVLDLFPAGVLQLWDSLANGYWHARRQAYLMTGPYHALEWVRVVADTVFLVFGVVPI
                     VIATLRSVLGGGRDDASPVTLPVAAARP"
     gene            complement(9666..10172)
                     /locus_tag="PLB01_03740"
     CDS             complement(9666..10172)
                     /locus_tag="PLB01_03740"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HQQ76444.1"
                     /translation="MKITKVATALLAAIFTAALTACASPSSPSKKISSVSSAPPVGVS
                     DASLGLEKTAVTGVPAPKPFDYVTKDPGDGKVLPRPFASAPPLIPHTVDGLLPITRAD
                     NGCVTCHAVDKAEPGGPVPIPASHYVDLRNAPTVKRAEIAGSRFVCTACHVPQASVKP
                     LVANGFTP"
     gene            complement(10158..12911)
                     /gene="napA"
                     /locus_tag="PLB01_03745"
                     /pseudo
     CDS             complement(10158..12911)
                     /gene="napA"
                     /locus_tag="PLB01_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
     gene            complement(12908..13165)
                     /locus_tag="PLB01_03750"
     CDS             complement(12908..13165)
                     /locus_tag="PLB01_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HQQ76445.1"
                     /translation="MNVSSIVVRVAPERVADGVRRLGELPGVEVRFTDADRGRIVVTQ
                     ESETTDEQEAGLRRIQALPGLLSAELVCHYFDELESEAEEA"
     gene            complement(13264..13557)
                     /locus_tag="PLB01_03755"
     CDS             complement(13264..13557)
                     /locus_tag="PLB01_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQQ76446.1"
                     /translation="MRPVKRVEIVVEALVLSRVTEIVDAAGIKGYTVIRNAGGKGGHG
                     ERFEDELVDTHRNAYLVVACDEAQLEPLLLTLRPLLSRFGGMCLVSDAWWVKH"
     gene            complement(13562..14539)
                     /locus_tag="PLB01_03760"
     CDS             complement(13562..14539)
                     /locus_tag="PLB01_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HQQ76447.1"
                     /translation="MDFSSIVQNVVSPAFLFFFLGFGAVLLKSDLEIPHPLPKFFSLY
                     LLLSIGYTGGAKLAHAGLSWSVALYIAVAMLMALAVPFYTFFILRKILSVYDAAAMAA
                     TYGSISIVTFIVGTEFLTRLSTPYGGYMVAIMSLMESPAVITGILLVRLFAPRGKDAT
                     ERPGVGHLLKESFLNGTVYLLLGSLVVGVLTGASAGKSLEPFTVGIFKGMVLFFLLDT
                     GMLAARRAKTLLVVGPKLVLFGIGAPLLNGGVGVLLAWAMGMGRGDALLFAILCASSS
                     YIVVPAAMRQAVPEANPGLFELLSLSVTFPFNISVGIPLYWAAIRLLIG"
     gene            14649..15113
                     /gene="nrfH"
                     /locus_tag="PLB01_03765"
     CDS             14649..15113
                     /gene="nrfH"
                     /locus_tag="PLB01_03765"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HQQ76448.1"
                     /translation="MPVKSRRGFAVLPVPLTLAAAAAGLALGVGGYAFWYAKGSSYMG
                     NDPATCANCHVMSAHVAGWQAAPHHNAATCNDCHTPAGPVSKYLVKASNGWHHSLAFT
                     LGGFPDVIRARPESSAVVEANCRRCHSDLVDSMTQGGDVSCIRCHASVGHQR"
     gene            15144..16802
                     /locus_tag="PLB01_03770"
     CDS             15144..16802
                     /locus_tag="PLB01_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HQQ76449.1"
                     /translation="MSAENAEPGKKKSGLLIALVAGAFALGALGAAALLVNIAEKKQE
                     AKNAYVKLAEVTENDVDPAKWGVNWPREYDGYKRTSEPSHTKYGGGTGGAEGVVPPQK
                     AARDPWLTRIFAGYLFAVDYRDRRGHAFMLFDQENTKRNVQAEGKQSGNCLHCHASIM
                     PLYRKLGKEAAPQASEAEQLQKGLEAVGALGYWDAHKQLEQTTGGKAHPVSCVDCHDP
                     KSMELRVTRPAFVAAIQKLAASKGAVGHLPSIERWRSGDRARAYDPNVDATRQEKRSY
                     VCAQCHVEYFCGKGTTIFFPWAEGLKVEDMEKQYNSIEVKGARFKDWTHAETGMEVLK
                     AQHPEFEMWSQGIHARSGVACADCHMSYRRDGAQKISEHWVRSPLLSANRSCASCHPY
                     TDEELKSRVEAIQDRHFALMSRAGQAAVAMIDAIVAVRKPYDDQNRPAAAAKAKETLE
                     KNADFAKLGKEDQDKKMAAETKANLLAAWRAVVDKDAAIKELGELQRAAQWRLDMVAA
                     ENSMGFHAPQEMARILGESIDLSRQAQVKAGEVLHGKAVKTASR"
     gene            16903..17364
                     /locus_tag="PLB01_03775"
     CDS             16903..17364
                     /locus_tag="PLB01_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQQ76450.1"
                     /translation="MRVQDVMTPHVRTISAFETAETAYASMKALNVHHLVVRDDEAAL
                     VGIVSSHDLGAAKEDGFRRNHRVADVMSTNVVTADPEMSIRQAANLMRGRSIGCLPVV
                     QPGRTPRLVGILTTSDLLEVLGHGVDRPTHPERRVLKARAPRTAPKIKRMH"
     gene            17480..18283
                     /locus_tag="PLB01_03780"
     CDS             17480..18283
                     /locus_tag="PLB01_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76451.1"
                     /translation="MNKPAVRFALALLVTLASAVWQRRSGPTYPARGRVAVGGSSVDL
                     KLTRTHGGPGDQPVRVAAPDPAFTGVVAFRRYPTSDEWTILPMAREGADLVAALPHQP
                     PAGKLEYQVRLAKGTETAVFPPRPAVTRFKGDVPTGILVPHVLCMVLAMFFSAAAGLA
                     ALAGAPTRRLALVTIGLIAVGGFILGPIVQKMAFDAYWTGVPFGWDLTDNKTLIAGLF
                     WAGAILVQRPGRPARIEIMAAAVTTLLVFAIPHSTWGSEIKWEAVKKAA"
     gene            18391..21963
                     /locus_tag="PLB01_03785"
     CDS             18391..21963
                     /locus_tag="PLB01_03785"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HQQ76452.1"
                     /translation="MKPRVRKGLLFLWLTLTWIRPAVAGVPEKVWETRVLKPAPQGYP
                     SRIVRNVRALADGSLMALGPDGAAASALRYSASGQTLSTVTLQAGRGGPGAIDAFGNF
                     VITTPFDRSAGFSVMRFDGTTGATLWAKIFVVDAPVAGFADPEAVVLDPRGDVLIAAR
                     SGTPNAFFAAVYKLDGVTGSLLWGPSLLAESSGSDPILLRTDGAGRIFLAMTQDDPVT
                     YRKLAVTQLDPSTGVIRWGPVTLGRTFTDSLVDAAVDGNGDVVVTGKYGGITATLKVG
                     GSSGALLWGPILETAAAPKGVVCDARGEVVVAIEATPSSPQTLLVGYDGSSGERRWGP
                     VLFPAAYASDAKLRVDATGNAFVSAKIPRPGLYPTDIGTAAFDVNTGARLWGPVEMGG
                     PDNSYDELGALELDLAGAVVVAGSVNSGAAMIVHAYDRATGTTLWGPRYFWGGGNGSD
                     IPSSSDTDAAGDIFVAADHSWDGDSLVTKVEGRSGRILWGPVPLGLPNAGVTKPKVHG
                     TTPGDAVVARWNGRGSSVVMLNGLNGTLRWTRSFAGVAFPLAVPGPGGDVLFAGLASG
                     TNLMIWKLAGSTGATLWGPANLGAILGIPEDGTVDANGDLVLAGEIGRSMAVVKIGGA
                     SGSLVWGPTVVTSGGNSIDHAVKVLIDASANVLVSGTNSEGPASFQTVTKLRGTTGVP
                     LWGPWTSAASGFFGGNSDMALDASGNVVVTGGGTFRLDANTGAAVWASSTVAQFVQFG
                     GDGNVVLAASSFDDSLFDWALTFQKLDSATGLPLWAPLHRPHASSLAALANVEGDPVF
                     VAATTDLEKATLLGRYSDGLTISPATSSPGAVLCGRPFSFSFHADNGTPPIQWNLLSG
                     TLPSGLTLGSDGTLSGTTSDVGEFPVIVRITDGTGRTTTREVTVRVLAAAAVAAIHVA
                     TSPACQGPDRVLSVIGSYDAYEWLPGGETTPSIIVRPVERTTYGVTVRTGSCLANGAV
                     ILEPGPMLLTPTIAAPERALAGSPGLTASATERPGSTYFWSISNGTIESGQGTSRIQF
                     TAGVSGPLSLSVSETDAAGCLHPEASRRVMVVSSAAGLLFYAVPPCRLLDTRLPSPNP
                     VTLRLDFGYGVFLAGSCGIPFAAQSVVINVTVTAPTAAGDLRIAAGRPSAPPDVSLLS
                     FQPGQTRAVSTLVSLPSDGSNLVTLWGAGSPTVHVVLDVSGYFQ"
     gene            complement(21999..23525)
                     /gene="ilvA"
                     /locus_tag="PLB01_03790"
     CDS             complement(21999..23525)
                     /gene="ilvA"
                     /locus_tag="PLB01_03790"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HQQ76453.1"
                     /translation="MKRAKTGEEYLERILSARVYDVAVETPLDAAPLLSRRTGHTVLL
                     KREDLQPVHSFKLRGAYNRMSRLSPAALRRGVIAASAGNHAQGVALAAQRLGCPATIV
                     MPVTTPRIKVDAVKSRGARVVLTGDSYDEAYAEARRIARAEKRTFVHPYDDPDVIAGQ
                     GTIGMEILRQRPAGVDAIFVAIGGGGLAAGVATYVKRISPKTRIVGVEPLDSDAMWRS
                     LRAGRRVRLARVGLFADGVAVKQVGKETFRLCRDLLDEVVRVDTDAICAAIKDVFEDT
                     RSILEPAGALAVAGLKAWAEKRKAKGKTLVAVACGANMNFDRLRFVAERAQLGERREA
                     ILAVTIPERPGSFREFCAIVGRRSITEFNYRYADATDAHVFVGVEVESPRQTAALVGA
                     LKKRRLPVLDLTENEMAKLHVRHLVGGRAPAAHDERLFRFEFPERPGALLNFLNHLRS
                     DWNISLFHYRNHGADYGRVLAGIQVPRKDDAKFRRFLSELGYRHTDETKNPAYRLFLL
                     "
     gene            complement(23542..24507)
                     /locus_tag="PLB01_03795"
     CDS             complement(23542..24507)
                     /locus_tag="PLB01_03795"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HQQ76454.1"
                     /translation="MESKWIWKDGDFVRYEDATLHFLTPGLHYGIGVFEGIRCYQTKS
                     GPAVFRLGEHIRRLRLSAKALGFDSLPFSEEELIEATKGTVAKNGFSECYIRPMIWLD
                     AGGWNLNLDGGRPAIGIAAWEWKNYLGEAALERGVRANVSSYTRHHPNVSMTKAKISG
                     NYVNSFLAKTESVRLGFDEAIMLDPAGYVAECTGANLFVVKDDRIVTTPSAPILEGIT
                     RDTLLTLAKELGFDVVEQTIARDQLYMADEVFVCGTAAEVVALREIDFRTIGSGTAGP
                     VTLALQNAYHAAVRGAHARSKEWCTPVREARRYPAPVRLVRGRRR"
     gene            complement(24521..25645)
                     /gene="leuB"
                     /locus_tag="PLB01_03800"
     CDS             complement(24521..25645)
                     /gene="leuB"
                     /locus_tag="PLB01_03800"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQQ76455.1"
                     /translation="MGKRARIAVLPGDGIGPEVTAGAVAVLKAVEARFGHTFVFSEWH
                     LGARALREKGTPLPEDTLVACRDADAVLMGAVGDPAYDLAPYDQRPEYGLLQLRKALG
                     VYANIRPARLYKALADSGPLKPEVARGLDLVVVRELTGGLYFGTPRSIDLAAGTAVNT
                     LPYTRPEIERIAEMAFRLAATRRNKVTSVDKSNVLETSRLWRAVTTEVGKRHPDVTLE
                     HQLVDSCAMNLVLFPSAFDVLVTENLFGDILSDECGALVGSLGLLPSASLGDGPGLFE
                     PVHGSAPQIAGRDLANPIGAIATAAMLLGDGLGLHEEAAAVHRAIEAALERGARTADL
                     AQKGTRALGTREMTRAIADEVAAATTQMAPGREAACGAPK"
     gene            complement(25646..>26746)
                     /locus_tag="PLB01_03805"
     CDS             complement(25646..>26746)
                     /locus_tag="PLB01_03805"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQQ76456.1"
                     /translation="VAKAVRRPTVAALARAVDADVDRALQALDGCARPRLHTFIATSD
                     LHLAHKLHMSRGQALEAAARAVARARRHVDDVEFSAEDATRSDVDYLCAVVAAVIRAG
                     ATTVNLPDTVGYSTPDEIYRFFTTIRARVPGSDRVVFSAHCHNDLGLAVANSLAAIEA
                     GCRQIECTVNGVGERAGNASLEEIVMTLRVRPDKLPFTTGIVPGQIYAASQLLTELTG
                     EAVQANKAIVGRNAFAHEAGIHQDGILKDRRTYEIMRPEDVGVPKTTMVLGKHSGRHA
                     LKARCEALGIVPARHELDDLYRRMIALADRQKVVHDRDLVSLWSGMKRVVALRPAHHG
                     GNGHGNGARPAAPELRVDESPAVEEAGYGYGV"
BASE COUNT         3943 a         9374 c         9185 g         4244 t
ORIGIN      
        1 gacgttctgg gcgtacatct ggttctgcca gtacctcctg atctggtaca gcaacctgcc
       61 cgaggagact ccgtactacg cgctccgctt ccagggcgga tggacggccc tcttctggct
      121 caaccccgtc gtcagcttcc tcgtcccttt cgtgatcctg ctcgggtcgc gggcgaagaa
      181 gtctccggcg gcgctcgggc aggccgcgct cgtcgtcgtc ctcggccggt ggctggacgc
      241 gtacgtcatg gtcgcgcccg ccgtgggtcc cctcccctct ttcccggccc acgccgtcgc
      301 ggcctcgttg ctcgtcctcg cgggaatggc tctcgccttc gaccggctcc atctcgggaa
      361 cctttagact gcctgcggac tctgtacggt catgcaggcc gacccgaacc ccctcgcgct
      421 cgccctcctc gacgacgaga ccgtcatccg ccgcgtgcag cagggggaga ccgccctctt
      481 cgagatcgtg atgcggcggt acaaccagag gctctacagg atcatccggt ccatcctcgg
      541 gtccgacacg gacgtcgagg acgtgctgca ggaggcgtac ctgaaggcgt acggccacct
      601 cggccagttc gagggccggt cgagcttcgc cacctggctg acgaagatcg ccgtctacga
      661 ggcgagcgct cgcgcccgga aaggcaggcg gttccagccg ctggaggtcc ccgtgggaga
      721 cgacgagaac agagtcgcgg agctccgctc cagccttccg gacccggagc aggacgcttc
      781 ccgccatgag ctcaagcgcc tgatcgagga ggcggtcgac caccttccag acatcttccg
      841 cgaggtcttc gtcatgcggg agatcgagca gatgagccag gccgagaccg ccgagtgtct
      901 cgggctgaag gaagagaccg tgaagacgcg cctccatcgg gcgcgccggc agatcgagag
      961 ccacctgctc gcacgcgccg ggcaggggat ccgcacggcc ttctcgttcg gagactcccg
     1021 ctgcacccgg atcgtcacga acgtcctgca acgcctcgcg gtggggcgaa tcccgcgcgc
     1081 ctgacgcccg cccgcgagtc ctgacgcgga cggatccgtc cgcttgttca atccctcaac
     1141 gcatcgaccc cctggaggag aaccgcatgg acacccgact cgtctccgct ttctggaccc
     1201 ttcgaatcgc cttcgggctc acggccttcc tggcgggtct cgacaagttt ctgaacctgc
     1261 tgacgaactg ggagcaatac gtcagcccgc tcatcctgaa ggtcgttccc ctctccgccg
     1321 gagcgctcat gcgcgtcgcg ggcgtgatcg agatcgccgc cgggatcgcg atcctcgccg
     1381 gcctcacgcg gctcggcagc tacgtcgtcg cggcctggct cgcgctcatc gcgctcagcc
     1441 tcgtgacggg cggacgttac ttcgacgtgg ccgtgcgcga cctcgtgatg gcgtgcggct
     1501 ccttcgccct cgcccgtctg agcgaggtcc gcgagcacgc ggccgctccc tcggcggccc
     1561 gcgccggcgc gctctcggcg tcctcggtct gacggcgatg agacgagacc tgcggctggc
     1621 cggcgaaccc gagagacacg acgagggcga ggacgccgtc gtttcgctcc tcagaatgat
     1681 cggagaggat ccctcgcgcg acgggctgct cgagactccc cggcgcgtcg tccaggcgct
     1741 ccgcgagatg acggccggat acggcgaaga gccctccgag atcctctcga agacgttcga
     1801 ggagtcgagc gacgagctca tcgtccttcg cggcatcgac tttcattcca cctgcgagca
     1861 ccacctcctc ccgttctacg gcacggcatc cgtcggctat ctcccgggcc tggtcgtcgg
     1921 catcagcaag ctcgcgcgtc tcgtccactg cttcgcgagg cgtctccaga tccaggaacg
     1981 cctgacgcgg cagatcgccg acgcggtcgc ggagcacctg gacgcgaggg ggaccgcggt
     2041 cgtcgtcaaa gcgcaccacc tctgcatggg ttgccgcggc gtgaagcagc cgggcaccga
     2101 gatgatcacg tccgcgatgc tcgggaccct gcgcgaagac gccgtgacgc gcggggagtt
     2161 cctccggctc gccggcgtcg gcacgtagac acggaaacgc gacgttccgc ctcgccaacc
     2221 ggcccgaaac gcctcgggca agaagggatg cgccatgacg aagaaagctt ctctcttcac
     2281 gctcctcgcg ctcgttccgc ccgccgtcgt cctcgtggcg gcgctccgca gcgaccgcgc
     2341 cgcccttgcc cagtcggccg cgccgtcgca cgacgtcgcg atgcgctcga gggaatcggc
     2401 ggacgacgac gaccgctcga cgatggcgag cagccacagg atgctggacg agggacggcg
     2461 gatcttccgc tacgacacct tcggcgacga ggcgttctgg ggcgacacgc tccggctcca
     2521 cgaagcgatc gccggctccg cgaacggagg agtcggcccg ggggtgagcc cgaagaccgc
     2581 gctcgccgtg ggcctgaagg tcgactccga cgcgctgccc ccctcggtcg tgcaggggat
     2641 ccggtccgga aagatcaacc tggacgaccc ggcgacgacg ctggcactgc tgcagctcga
     2701 cgccgtcgtc ggtgtcacgg gccgcttctc ccccgggaag cagatcgtct cgctcgggat
     2761 ccagtgcgcg ttctgccatt ccacggtcga cgattcgctc gcccccggca tcgggcgccg
     2821 cctcgacggc tggccgaacc gcgacctgaa cgtcggcgcg atcgtggccc tctccccgga
     2881 cctctcggcc gtgaaccggc tcctgggagt cgacgacgcg acgacgcgca cggtgctcgc
     2941 gagctggggt cccgggaagt tcgacgccga gctcttcctc gacgggaagg tgttccggcc
     3001 ggacggccgg tcggccgcga cgctcatccc ggccgcgttc ggcctcaccg gcgtgaacct
     3061 tcacacgtac accgggtggg gctcgatccc gtactggaac gccttcgtcg cggtcctcga
     3121 gatgcacggc aagggcaact tcttcgatcc gcgcctcgac gaccccgtgc agttccccgt
     3181 cgcggcccgc gcccgttccg gccacgcaca ggtcgccgag ggcgaggacc aggtcaccgc
     3241 gaagctcccg gccctccatt actaccagct ctcgattccg gcgccgacac cgcgggttgg
     3301 cagcttcgac gccgcggccg ccggacgcgg aggcgtgctc ttcgccggga aggggcagtg
     3361 cgcctcctgc cacacggagc cgctcacgac cgagccgggc tggaacatgc acacgcccga
     3421 ggagatcggc attgacgact tccagtccag ccgctccccc gacaagcgct accgcacgac
     3481 gcccctccac ggcctcttca cgcgcgcgaa gggcggcttc tatcacgacg ggcgctttcc
     3541 gaccctcctc gacgtcgtga atcactacga ctcgttcaag ggcctctctc tgacgccggc
     3601 cgagaagagg gacctcgtcg agtacctgaa gtcgctgtaa ggtgagccgg atgcccgact
     3661 tcaagtacgt cctggtcggc ggcgggatga cggcgcacgc cgcggcactg gggatccgct
     3721 cggtggatgc gagcggctcg atcctcctcg tcggcgacga gccggaagga ccgtacgcac
     3781 gcccgccgct cacgaagggt ctctggacgg gacaggacga ggcctcgatc tggctgcccg
     3841 cggtacccgg cgtcccggtt cgcgccggcg cgcgcgtcgt cgccgtcgac cgcgccgcgc
     3901 ggcgcgtcgt tctcgacgga ggagagacga tcggatacga gcgcctcctc ctcgcgacgg
     3961 gcggaacgcc ccgccggctg cccttcggcg gcgacgacgt cgtctattac cgcaccgtcg
     4021 cggactaccg gcgcgtccgg gcgctgccgc cgggccggca cgtggcggtc gtcggcggag
     4081 gcttcatcgg ctcggagatc gcggcctcgc tgtcgacggc cggcacccgt gtgacactgg
     4141 tctttcccga ggagggaatc ggcgcgcgcc tctttccgcg cgatctctcg ctctacctga
     4201 acgatttcta caggcagcgc ggcgtcgagg tccggccggg ggaacgcctc accgggctcg
     4261 aggcgcacgg ccccgggttc acggtagcga ccgaccgcgg ccggatccag gccgacctcg
     4321 tcgtggcggg gctcggcatc gtcccgaacg accgcctcgc ggccgaagcg gggctcgacg
     4381 tagacgacgg catcctcgtg gacgagtccc tgcgcacctc cgacccggcc gtcttcgccg
     4441 ccggcgacgt cgcgcggttc tggagccccg cgctcggccg gcgcatccgg gtcgagcacg
     4501 aggacaacgc gaattccatg gggcgggagg ccggacgcgc gatggccgga gccgacgtcg
     4561 cctaccgcca cctgcccttc ttctactcgg atctcttcga cctcggatac gaggccgtcg
     4621 gcgtcctcga cccgcgcctc gagaccgtcg ccgactggaa ggacccgttc cgcaagggtt
     4681 tcgtctacac cctcgacgac ggccgcgtgc ggggcatgct ggcctggggc gtcttcggca
     4741 agatggacga agcgaggagc ctcatcgcgg agccgggccc gcacgccccg gagtctctcc
     4801 gcgggagaat ctccgcgtag ggacgaggag gtccggtgag tgacaaggcg aggatcgacg
     4861 cacacgtcac gactcccgag gcgtacgaga cgctgctggc gctcgaggac tatctcgatc
     4921 gctgcggtct cgagcgttct ctgatcgagc tcgtcaagct gcgcgcgtcc cagatcaacg
     4981 gctgcgccta ttgcatcgac atgcacacga aagacgcccg ggccgccggc gagtcggagc
     5041 agcgtctcta cttgctcgac gcctggcggg aatccccctt ctattcgtcc cgtgagcggg
     5101 ccgcgctcgg ctggaccgag tccgtcacgc gggtctcgga aacacacgtg cccgacgagg
     5161 tgtacgacga ggcgcggcgg cacttctccg agaaggagct cgtcgacctc aactgggtgg
     5221 tcgtggcgat caacgcctgg aaccggctcg cgatcccctt ccgcaccccg cctggcattt
     5281 accagccggc cccttagggg gcggctcgtg gcgcgggcgt gctacgagaa atcaaacctg
     5341 gccagccgct ttcgcctcca gagacttcag ctgggctcga agcgccttct cccgggcgta
     5401 gcgttccgtc acgtcgcgga acaccgccac gacccactgg atccggtcgc cggcatcctt
     5461 cagcagctgg atcgagaact ccacggagat ctgacggccg tccttgtgga gcgcaggcac
     5521 ggcgagcatc ctgccttcgc cgtagcgggt gacgccggtc ttcatggtcg cctcccagcc
     5581 ggcccagtgc cggccgcgga gccgctcggg gatgatgaaa tccatggaga ttccgaccgc
     5641 ttcggcggcg cggaagccga acacccgctc cgccgcggcg ttccagtagc gcaccgtacc
     5701 cgtgggatcg ctgatgagga ccgcgtcggg cgagccgtcc aggacgcggc gaggaagatc
     5761 gtcggggagc gccgtcatca ctttgaggac tccttcatca tgactgcctc gccgcgcgga
     5821 acgtcccctc gtagatcacg gggccgccgc gcgcgatcgc gccggccggc gggcggttgt
     5881 actccgtgag cgggcccggc gggtctttct cgaacccgcc gcgcgcgagc tccgcgagga
     5941 cctccgcctc ggtcaggaag cactgcggct cgcccgcgaa ccgggtgaac acgaacggct
     6001 ccccgggcac gggccggtcg tccgggggga gggtggcgcg ggagaacgtg aagaggaaga
     6061 ggccggcgcc cggccgggcg acgcgccccg cctcggcgat cgcgcggcgc atctcggagg
     6121 ccgaccgggc gagattccag atcccgtggg cgacgacgag gtcgacgctc gcgtccggca
     6181 gcggcagccg atccatgggt gcccgcgcga gcgtgactcg cccggcgacg ccttcggatg
     6241 cgacccggcg gcgcgccgcc gcgagcatgg gccacgcgat gtcggtcccg acgacggcgg
     6301 cgccttccgc cgccatgggc accgcgttgc gggcggctcc gcagccgagg tcgaggacgc
     6361 gaagaccggg ccgccgggcg agctcgaggc ggacgaaggc gaggagaacc tggttcgcgg
     6421 cggccttcga gaagccctgg acgacgccgg cgtcctccca gcggcccgac acggtcagcg
     6481 gaccggctcg gtcggcgcgc gagacgccgc ggcgctcatt tcgaggctga cgacccgcgg
     6541 gaacaggacg ttgttctcga ggtggacgtg ctcgtgcagc tcgcgttcga agcgcacgag
     6601 cccctcgtag agcgcctgat aggtcgcaca ggcgtcgtcg gggaccacga acccgcgact
     6661 cagcgcgcgc aggctcgcga gagcggcgcc ggcgcggtcg tgctcttcct cgagggcgtg
     6721 cagggctccc ttcacgttct cggcgggcgc cccggactcc gcgagcaggc ggacggaggg
     6781 gaacacccgg tcctcctccg tcgcgaggtg cggctccagc tcggaccgca gctcgcggta
     6841 gacgcgcgcc agctcgacga gctcggggtg gcgcatcccg tggacctcgg ccaccttgtc
     6901 ggccaggcgt ccgaggagcg gaaggtcctc ccgcaaggct ccgtggtaac gggagacgat
     6961 gtgatcgaga agcgtcgcga gcggagcgtc ggtgaagatg cggtcttccg gcacctcacg
     7021 ggcggcggcg gcctcgatct cggccgcgac ggcctccacg tgggctccgt gctcgcgaca
     7081 ggcctcgccg agggggcggc ggccgccgca gcagaagtcg atcccatggc gctcgaagac
     7141 ctcgatgctg cgggggtggt gggccgcgat gtccgcgacg aacgtctctg cggtgattct
     7201 caagagatct tcctccgtcg gcgaccatca tgccgagtcc tcgagggcgg cgggatgaca
     7261 ggcgtcatat gccgtgagag tcagagcgga ggaatcgagg tcacccgttg acgttggaat
     7321 gcgtctcagt cacgatcatc tgatgagcga acacgaaaga ctttccccct ggtggcggca
     7381 cggcgccatc ctcgccatga tcttcggatt ctcgatcctg tcgatcgtga cggtgcaaac
     7441 gtacaagaac gcgccaccga tcccggaccg ggtcgtcgac gaatccggcc gaacgatctt
     7501 caccggggcc gacgtcctcg cgggccagga cgtcttcctc aagtacggcc tcatggagca
     7561 cgggacgctc tggggccacg gcgcgtacct cggtccggac tacacggccg agacgctgca
     7621 ccgcctcgcg gaagtctggc gcgacacgct cgcgaagagc cggtaccagt cggcgtacgc
     7681 cggcctcgac gcgggacgtc aagccgcgat cgacgccgag gtccggaccg cggcgaggac
     7741 gaacacatgg gacccggccg cgaagacgat cacgtttccg cccggtgcgg ctgcggcgtt
     7801 cgcggtcgcg aagacggagt gggcggcgta cttcggcggc gagaccgcgg cgcccggcct
     7861 tcccgcgcgc tacatcaaga acccaaagga actcgacgcc ctctccgcgt acttctcctg
     7921 ggcgacgtgg gcgacggtcg cgagccgtcc gggcaaggac tactcgtaca cgaacaactt
     7981 cccgtacgag ccgctcgtcg ggaactcccc cacgtccgcg acctatctct ggagcgggct
     8041 ctctctgatc gcgctcctcg gcgccctcgg cacgattctc ttcgtgttcg gccgcttcga
     8101 cttcctgggc tggaaagccg agagcggagc cttgcaccac cacgacccgc cccgcgccct
     8161 gaccctgacg cccagtcaga gggtcgtcgg ctggttcctc gcggtcgtcg cggtcctctt
     8221 cctgctccag acgctcgcgg gaggcgcgct cgcccactat cgcgtcgaac cgggcgcgtt
     8281 ctacggtttc gacctcgcgt cgatccttcc gtacacgctt ctgcgcacgt ggcacctgca
     8341 actcgcgatc ttctggatcg cgaccgcgtg ggtcgcgggc ggcctcttcc tcgcgccgct
     8401 cctcggcggc ggcgatccga aagggcagaa ggccggggct ctcgtcctcc tcggcgccct
     8461 tgcggtcgtc gtgttcggga gcctgttcgg cgagttcctc ggcctgcgca acgcgctcgg
     8521 gagattctgg ttctggttcg ggcaccaggg ctccgagtac ctcgacctcg ggcgcttctg
     8581 gcagatcctc ctcgcggccg gcctcgtcct gtggctcgtc ctcatgttcc gggcccttcg
     8641 gcccgcgatg aagagcccga cgaccggcac cctcgcgtcc ctcttcctct acatcgcggt
     8701 cgcgattccc ctcttctacc tgccggccct cgtctacggc ccgcacacga acccggccgt
     8761 gatcgacaac tggcgtttct ggatcatcca cctctgggtc gagggtttct tcgagctctt
     8821 cgcgacggtt ctcgtcgcgg tcatgttctt ccagatgggg ctcgtgagca cgaagacggc
     8881 gacgcgcgtc gtctacctcg acgcgatcct ctatctcggc gccgggatcg tcgggacggg
     8941 ccaccactgg tacttcaccg gccagacgac gctcaacatg ggcctcgccg cgacgttctc
     9001 cgcgatggaa gtcgtccctc tcacgctcct gacgctcgac gcgtgggact tcatccggct
     9061 caaggatcgg acgtgcgggt cctgcggcgt gacgctctcg gccgcgcaga aatgggcgat
     9121 ccacttcctg atggcggtcg gcgtctggaa cttcgtcggt gccggcgtct tcggcttcct
     9181 catcaacctc ccggtcgtct cgtacttcga ggtcggaacg accctgacgt cgaaccacgg
     9241 ccatgcggcg atgttcgggg tgttcgggat gctcgcgctc gccgtcgtcg tctactgcct
     9301 gcgcgccctc cagagcgagg tgcgctgggc ccgcgtggaa ggcttcgtga agacgggctt
     9361 ctgggggctg aacgtcggcc tcgccctcat ggtcgtcctc gacctcttcc cggccggggt
     9421 cctccagctg tgggactccc tcgcgaacgg ctactggcac gcccgccggc aggcctacct
     9481 gatgaccggc ccataccacg cgctcgagtg ggtgcgcgtc gtcgcggaca cggtgttcct
     9541 cgtgttcggc gtcgtcccga tcgtcatcgc gacgctgcgc tccgtcctcg ggggcgggag
     9601 ggacgacgcg tcgcccgtga cgctccccgt cgctgccgcg cgcccctagg ccttgcggcc
     9661 gtcgttcagg gcgtgaagcc gttcgcgacg agcggcttca cgctggcctg gggcacgtga
     9721 cacgccgtgc agacgaaccg gctccccgcg atctcggcgc gcttgacggt cggggcgttc
     9781 ctcaggtcga cgtagtggct cgccggaatc ggcacggggc ctcccggctc ggccttgtcc
     9841 acggcgtgac acgtcacgca gccgttgtcc gcgcgggtga tcgggagaag gccgtcgacc
     9901 gtgtgcggga tgagcggtgg cgccgacgcg aacgggcgcg gcaggacctt gccgtccccg
     9961 gggtccttcg tgacgtagtc gaacggcttc ggggccggga ctcccgtcac cgcggtcttc
    10021 tcgaggccaa ggctcgcgtc cgaaacgccg accggggggg cggaagagac cgaagagatt
    10081 ttcttagaag gtgaagaggg ggaggcgcac gccgtcagcg cggcggtgaa tatcgctgcc
    10141 agaagcgcag ttgcgacctt cgtgatcttc acggcgcact tcttgaagtc cgtctccttc
    10201 gagatcgggc acgtcgcgtc gagggtgacc ttgttgatga ggatcgactc gtcgaaccac
    10261 ggcacgtaca cgagcccgcg cggcatcctg tagcggcccg cggtctcgac ggccacctgg
    10321 attttcccgc ggcgggactc gatccacgcc acgtcgttgc gcctgaggcc gcgcgcggcc
    10381 gcgtccttct cgtgaatgta gaggagcgcg gacggcatcg ccgcgttcag ctccgggacg
    10441 cggcgggtca tcgtccccga gtgccagtgc tcgaggacgc gcccggtgca gagccacagg
    10501 tcgtacacgg cgtcggggac ctcggcgggc gccgcatacg ggcggaagaa gatcttggcc
    10561 ttgccggcga gcccgacctt ctccgtgccc gtgggcttgt cgaggtctcc cttcggcagc
    10621 gccttcatcg ccttgccgta gaagtcgaag cccgagccct tcttcgcgta cgggtcgtaa
    10681 gcctcgttga agcgccaggg cgtctccttg ccgtcgacga cgggccatct caggccccgc
    10741 acgtcgtccg cgaggtagcg gtcgaagggc gcgaggtcgt gtccgtgccc gcgcccgaac
    10801 cccgcgtact cctcgaagag cgccttctcc gggaaccacg ccgcgccgag atgcgcggcg
    10861 gtcgcgttcg cgtgcccctt cgcgacgggg tccggccagg cgaccttccg gttcgccggc
    10921 gtcgcgaaga ggacgtcgaa gagcgtcgcg tcgggggcgt agcccatctt cgcggcctcg
    10981 tcgaggacgg aagggagccg tccgtccgtg aagccctcgg tcttgaggcc cgggagcggc
    11041 tgctcgcccc agacgtcgcg caccttgaag cgcttggaga actcgaggat ctgccagacg
    11101 tcggtcttcg cgtcgcccgg cgccggcacc tgctgccgcc agagctgcgt gcggcgctcg
    11161 gagttgccgt agccgcccca cttctcgaag atcatcgcgc tcggcaggat caggtcggag
    11221 accttcgcgc tgatcccggg gtacgcgtcc gagacgacga cgaacgtgcc gggctggcgg
    11281 gcggccgcga gccagtggtt gttgttcgcg gtggactgga acgggttcgt gacctggatc
    11341 cagagccact tgagcttgcc gtcctcgagg tcccgcatca tctccgtgat gtggctcccg
    11401 accttcgggt tgatcgtctt cgccgggatc ttccagtgct cctcggcgtg ggcgcggtgc
    11461 tcggcgttgt ccacggtcat gtccgcgggg aggcggtggg agaacgtgcc gacctcgcgc
    11521 gccgtgccgc aggcggaggg ctgccccgtg agcgagaacg cccccgcgcc gggcttcgcc
    11581 tgcttgcccg tcaggagatg ggccatgtac gcctgttcgt tgacccacgt tccgcgcgtg
    11641 tgctggttga agcccatcgt ccagaacgac acgaccttcc tcttcgggtc ggcgtagacg
    11701 tcggccagcg ccttgagctt ccgcgcgtgc tcctcgagcg gctcttcgga gttgcccttc
    11761 gtgagcgcgg cgacgaagtc gagcgtgtac ggctcgaccg ccttcttgaa gtcctcgaag
    11821 gagatgagcc agtgcgctcc cgccttcgcg gcgtccttct ggggccgcgt gtgcgtgacc
    11881 gacgggtcga ggccgcggcc gatcgcctcc tccttcgtga ggacgaccgt ccgctcgcgg
    11941 tcccgcgtgt ccttctcgga cgcggtcgcg tggtcctccc ccggccgcat cccgaacccg
    12001 atgtcgacgg ccccggccgc gaagacgcag tgcttctcga cgaagtcctt gtccaccgcg
    12061 ccgcgggcga cgatctcgcg cgcgatgtag ttccagatcg cgaggtccgt gttcggcttg
    12121 aagaggatct cggtgtccgc gcccgccgac gtcggcgtcg tgtaggtcgt gaggttgagg
    12181 atccggtact cggggcgcgc gagccgcgcg tcgacgatgc gcgaccagag aatcgggtgc
    12241 atctcggcca tgttcgcgcc ccaggtcacg acggcgtcgg tcttctcgat gtcgtcgtag
    12301 cagcccgagg gctcgtcgat gccgaacgtc tgcatgaagc cggcgacggc cgacgccatg
    12361 cagtgccgcg cgttcgggtc gaggttgttg ctgcgccagc cggccttcac gagcttcgcg
    12421 gccgcgtagc cctccatgat cgtgtactga cccgagccca tcacgccgac gccgctcggg
    12481 ccgagctcgg cgtggaccct cttgaactgc gcctccatct cctggaacgc gcgttcccag
    12541 ctgacgggga agaagtcgcc gttcttgtcg aacttgccgt ccttcatcct caggagcggc
    12601 ttcgtcaggc ggtcctcgcc gtagaggatc ttggcgttcg cgtagccctt gacgcagagg
    12661 aggccgcggt tcacgggagc gtcggggtcg cccttgatgg ccacgacgcg gtctgccttg
    12721 gtcccgacga gcacgccgca gcccgtgccg caaaagcggc agacgctctt gtcccacgca
    12781 accccgtcgg cggcgggcgc gcctcctgtc gccgcgaggg cgtcttcggc gaccggaatt
    12841 cccgcggcgg cggccgcggc gagcgcggca ctgcgcttga ggatctcccg gcgtgtctgt
    12901 ttctcgctca tgcttcctcc gcttcactct cgagctcgtc gaagtagtgg cagacgagct
    12961 cggccgacag gaggccgggc agcgcctgga tgcgccggag gccggcttcc tgttcgtcgg
    13021 tcgtttccga ttcctgcgtg acgacgatgc ggccccggtc ggcgtccgtg aaacggacct
    13081 cgacgccggg caactcgccg agccgccgga cgccgtcggc caccctctcg ggcgcgacgc
    13141 gcacgacgat gctcgagaca ttcaaggcgt gcgcgatctt agtcccgcag ggagggtccc
    13201 cgtatgacgg gcgtcatgcg gtgatttgcc ggcgcttcgt ggcgcggtcc gtcgcttcac
    13261 gcctcagtgc ttcacccacc acgcgtcgct cacgaggcac atcccgccga agcgggagag
    13321 gagcgggcgg agcgtgagga gaaggggctc gagctgggcc tcgtcgcagg cgaccacgag
    13381 gtaggcgttc ctgtgggtgt cgacgagctc gtcctcgaag cgctcgccgt gccccccttt
    13441 gcccccggcg ttgcggatga cggtgtagcc cttgatcccg gcggcgtcga cgatctcggt
    13501 gacgcgggag aggacgaggg cctcgacgac gatctcgacg cgcttgacgg gtctcatttc
    13561 gtcacccgat gaggaggcgg atcgccgccc agtaaagagg aatgccgacg ctgatgttga
    13621 aggggaacgt cacggacagg gagaggagct cgaagaggcc gggattcgcc tcgggaaccg
    13681 cctggcgcat cgcggccggg acgacgatgt aggagctgga cgcgcagagg atggcgaaga
    13741 ggagggcgtc gccgcggccc atccccatcg cccaggccag gaggacgccg accccgccgt
    13801 tgaggagcgg cgccccgatg ccgaagagga cgagtttcgg gcccaccacg aggagcgtct
    13861 tggcccggcg ggccgcgagc atcccggtgt cgaggaggaa gaagaggacc atccccttga
    13921 agatcccgac ggtgaagggc tcgaggctct tcccggcgct cgccccggtg aggacgccga
    13981 cgacgaggct cccgaggagg agatagaccg tcccgttgag aaacgactcc ttcaggaggt
    14041 gccccacgcc gggtcgctcg gttgcgtcct tgccgcgtgg cgcgaagaga cgcacgagga
    14101 ggattcccgt gatgacggcc ggcgactcca tgagcgacat gatcgccacc atgtagccgc
    14161 catagggcgt ggacaggcgg gtgaggaact ccgtcccgac gatgaacgtc acgatgctga
    14221 tcgagccgta cgtcgcggcc atcgcggcgg cgtcgtagac ggaaagaatt ttccggagga
    14281 tgaagaacgt gtagaaaggc acggcgaggg ccatcagcat tgccacggcg atgtagagcg
    14341 ccacgctcca tgaaagcccc gcgtgcgcga gtttggcgcc tcccgtgtaa ccgatcgaca
    14401 agaggaggta gagcgagaag aacttcggga gagggtgcgg gatctcgagg tcggacttca
    14461 ggaggaccgc cccgaagccg aggaaaaaga agaggaaagc cggcgagacg acgttctgga
    14521 cgatggaaga gaagtccatc aggcccgaga ggatacaacc gggcctttcg ggccccaggc
    14581 ccccgaggcg gggcccgaca tgacggccgt catgtgcgga acttcggccg agaccctaca
    14641 ttgacgtcgt gcccgtgaag tcgcgccggg gctttgccgt gctcccggtc cccctcaccc
    14701 tcgccgccgc cgcggccgga cttgcgctcg gcgtcggcgg ctacgccttc tggtacgcga
    14761 aggggtcgtc gtacatgggg aacgaccccg ccacgtgcgc gaactgccac gtgatgagcg
    14821 cgcacgtcgc cggctggcag gccgccccgc accacaacgc cgcgacctgc aacgactgcc
    14881 acacgccggc cgggcccgtg tcgaagtacc tcgtgaaggc gtccaacggc tggcaccact
    14941 ccctggcctt cacgctcgga gggttcccgg acgtcatccg ggcccggccc gagagctcgg
    15001 cggtcgtcga ggccaactgc cgccgctgcc actccgacct cgtcgactcg atgacccagg
    15061 gcggcgacgt ctcctgcatt cgctgccacg cctccgtcgg ccaccagcgc tgacacgcga
    15121 caccacccga gaaggagcac ctcatgtccg cagagaacgc cgagcccgga aagaaaaaga
    15181 gtgggctcct catcgccctc gtcgcgggcg ccttcgccct cggagcgctg ggcgccgccg
    15241 ccctgctcgt caacatcgcc gagaagaagc aggaggcgaa gaacgcatac gtcaagctcg
    15301 ccgaggtgac ggagaacgac gtcgacccgg ccaagtgggg tgtgaactgg ccgcgcgaat
    15361 acgacggcta caagcgcacg tccgagccgt cccacacgaa gtacggcggg ggcaccggcg
    15421 gcgccgaagg tgtcgtgccg ccccagaagg cggcgcgcga cccgtggctg acgcgcatct
    15481 tcgcgggcta cctcttcgcg gtggactacc gcgaccggcg cggccacgcc ttcatgctct
    15541 tcgaccagga gaacacgaag cgcaacgtgc aggccgaggg caagcagtcg ggcaactgcc
    15601 tgcactgcca cgcctcgatc atgccgctct accggaagct cgggaaggaa gcggccccgc
    15661 aggcgagcga ggccgagcag ctccagaagg gtctcgaggc cgtgggcgcg ctcggctatt
    15721 gggacgccca caagcagctc gagcagacga cgggcggcaa ggcccacccc gtctcctgcg
    15781 tcgactgcca cgacccgaag tcgatggagc tgcgcgtcac gcgtccggcc ttcgtcgcag
    15841 ccatccagaa gctcgcggca tcgaagggcg ccgtcgggca cctcccgagc atcgagcgct
    15901 ggcgcagcgg cgaccgcgcc cgtgcgtacg acccgaacgt ggacgcgacc cgccaggaga
    15961 agcgctcgta cgtctgcgcc cagtgccacg tcgagtactt ctgcggcaag ggcacgacga
    16021 tcttcttccc gtgggccgaa ggcctcaagg tcgaggacat ggagaagcag tacaactcca
    16081 tcgaggtcaa gggcgcgcgc ttcaaggact ggacgcacgc ggagaccggc atggaggtcc
    16141 tcaaggcgca gcaccccgag ttcgagatgt ggagccaggg cattcacgcg cggagcggcg
    16201 tcgcctgcgc cgactgccac atgtcctacc ggcgcgacgg cgcccagaag atctccgagc
    16261 actgggtgag aagccccctc ctctcggcga accgctcctg cgcgagctgc cacccgtaca
    16321 ccgacgagga gctcaagagc cgcgtcgagg ccatccagga ccgccacttc gcgctcatgt
    16381 cgcgcgcggg ccaggcggcg gtcgccatga tcgacgccat cgtcgccgtc cggaagccgt
    16441 acgacgacca gaaccgcccc gcggccgccg cgaaggcgaa ggagacgctc gagaagaacg
    16501 ccgacttcgc caagctcggc aaggaagacc aggacaagaa gatggccgcc gagacgaagg
    16561 ccaacctcct ggccgcgtgg cgggcggtcg tggacaagga cgccgcgatc aaggagctgg
    16621 gcgagctgca gcgcgccgcg cagtggcgcc tcgacatggt ggccgccgag aactcgatgg
    16681 gcttccacgc cccgcaggag atggcccgca tcctcggcga gtcgatcgac ctgtcgcggc
    16741 aggcgcaggt gaaggccggc gaggtcctgc acggcaaggc ggtgaagacg gcatcccggt
    16801 aggcggccgc cgcctgattc atctgctcca tgcgcggacg gccgcgcgga cccaccttcc
    16861 catcggaagc aggcgcgccg agagcgccgc caaggagggt ccatgcgtgt acaggacgtg
    16921 atgaccccgc acgtgaggac gatcagcgcc ttcgagaccg cggaaaccgc ctacgccagc
    16981 atgaaggctc tgaacgtcca ccatctcgtc gtgcgtgacg acgaggccgc gcttgtcggc
    17041 atcgtgtcgt cgcacgacct cggagccgcg aaggaggacg gcttccgccg caaccaccgc
    17101 gtggccgacg tcatgtcgac gaacgtcgtc accgccgacc ctgaaatgtc catccgccag
    17161 gccgccaacc tcatgcgcgg ccgcagcatc ggatgcctgc ccgtcgtcca gcccggccgg
    17221 acgccgcgcc tcgtcggcat cctcacgacg tccgacctcc tcgaggtgct cggccacggc
    17281 gtcgaccgcc cgacgcatcc cgagcgcagg gtcctgaagg cccgcgcgcc ccgcaccgcg
    17341 cccaagatca agcggatgca ctgagggctc cggccgccag gcggccgagc gctgagaaag
    17401 cgaacacccg ccggacgggg cgccccccgt ccggctgctt cattgacgcc gggggcggcg
    17461 tcccggtaaa tcggacggcg tgaataaacc cgccgtccga ttcgccctcg ccctcctcgt
    17521 cacgctcgcc tccgccgtct ggcagcgccg ctccgggccg acgtacccgg cgcggggacg
    17581 cgtcgccgtc ggcgggtcgt ccgtcgacct gaagctcacg cgcacgcacg gcgggccggg
    17641 cgaccagccc gtgcgcgtcg ccgctccgga tcccgcgttc acgggtgtgg tcgcgttccg
    17701 ccggtatccg acgagcgacg aatggacgat ccttcccatg gcgcgcgagg gcgccgacct
    17761 cgtggccgcc ctcccccacc agccccctgc gggcaagctc gagtaccagg tgcgcctcgc
    17821 gaagggaacc gagacggccg tcttcccgcc gcgcccggcc gtcacgcgct tcaagggcga
    17881 cgtgcccacg gggatcctcg tcccgcacgt cctctgcatg gtcctcgcga tgttcttctc
    17941 cgcggccgcg ggcctcgcgg ccctcgcggg cgcgcccacc cgccgcctcg cgctcgtgac
    18001 gatcgggctc atcgccgtcg gcggcttcat cctcggaccc atcgtccaga agatggcgtt
    18061 cgacgcgtac tggacgggcg tgccgttcgg ctgggacctc accgacaaca agacgctcat
    18121 cgccggcctc ttctgggcgg gggcgatcct cgtccagcgc cccggccgcc cggcccgcat
    18181 cgagatcatg gccgccgccg tcacgacact cctcgtcttc gcgatcccgc actcgacgtg
    18241 ggggtcggag atcaagtggg aggccgtgaa gaaggcagcg tgacgcacca cattcctctt
    18301 ctgcgaacct gaagtgggtt cgcgattgca cgcgccgatc gtggcagcga gacgctaggc
    18361 tagactggat cgagccgcgg agtgacacgc ttgaagcctc gggttcggaa gggtctgctg
    18421 tttctctggc tgactctgac gtggattcgg cccgcagttg ccggagtgcc cgagaaggtc
    18481 tgggagactc gcgtcctcaa gcccgctcca cagggttatc cctcccgaat cgtccgaaac
    18541 gtgcgtgctc tcgcggacgg ctctctgatg gccctcggcc cggacggcgc cgctgctagc
    18601 gctctgcggt attccgcgtc cggccagacg ctctcgaccg tcaccctcca agctggacgc
    18661 gggggcccgg gcgcaatcga cgcctttggg aacttcgtca tcacgacgcc cttcgatcga
    18721 tcggccggct tctccgtcat gaggttcgac gggacgacgg gcgcaacgct ctgggccaaa
    18781 atcttcgtcg tcgacgctcc ggtcgcgggc ttcgccgatc cggaggcggt cgtcctggat
    18841 ccgcgtggcg acgttctgat cgccgcgcgc tcgggaacgc ccaacgcgtt ctttgccgcc
    18901 gtctacaagc tggacggagt gacgggatcc ctgctctggg ggccgagtct ccttgccgag
    18961 tcatccggct cggacccgat tctgctcaga accgacggcg cagggcgaat cttcctggcg
    19021 atgacccagg acgatcccgt gacctacagg aagctcgcgg tcacccagct cgacccgtcc
    19081 accggggtga tccgatgggg gccggtcacc ctggggagga cgttcacgga ttcgctcgtc
    19141 gacgccgccg tggatgggaa cggggacgtc gtggtaaccg gcaaatacgg cggaatcacg
    19201 gccacgttga aggtcggcgg ctcctccgga gctctcctct ggggcccgat tctcgagacg
    19261 gccgccgctc cgaagggagt cgtctgcgat gcgcgaggag aggtcgtggt cgccattgag
    19321 gcgactccgt cgagccctca aacgcttctc gtcggatacg acggttcgag tggtgaacgt
    19381 cgatgggggc ccgttctgtt tccggcggcg tacgcgtcgg acgcgaagct ccgggtggat
    19441 gcgacgggca acgccttcgt ctcggcgaag attcctcgcc cgggcctcta tccgaccgac
    19501 atcggcacgg ccgcgttcga tgtgaacacg ggggcccgcc tgtggggccc agtcgagatg
    19561 ggagggccgg acaattccta cgacgagctc ggtgcgctcg aactggattt ggccggcgcc
    19621 gtcgtcgtcg ccggaagcgt caattccggc gcggcgatga tcgtgcatgc gtacgaccgc
    19681 gcgaccggca cgaccctctg gggtccgagg tacttttggg gcggcggcaa cgggtcggac
    19741 attccgtcgt cgtcggacac cgacgcggcc ggcgacatct tcgttgcagc cgatcactcg
    19801 tgggacggcg attcgctggt cacgaaggtc gagggacggt cgggacggat cctgtggggg
    19861 cccgtccccc tcggcctgcc gaacgccggc gtaacgaagc cgaaggttca cgggaccacg
    19921 ccgggcgatg ccgtcgtggc ccgctggaac ggccgtggtt cctcagtcgt aatgctgaac
    19981 gggctcaacg gcactcttcg atggacgcgt tccttcgcag gcgttgcatt tccgctggcc
    20041 gttcccggtc caggcggcga cgtcctcttc gccggattgg cttccggcac gaacctcatg
    20101 atttggaagc tcgcgggcag taccggtgca acgctctggg gacccgcgaa tctcggcgcg
    20161 atcctcggaa ttccggagga cgggaccgtc gatgcgaacg gagatctcgt actcgcgggc
    20221 gagatcggac ggtcgatggc ggtcgtcaag atcgggggcg cgagcggctc cctcgtgtgg
    20281 gggccgaccg tcgttacgtc gggcggcaac tcgatcgacc acgccgtgaa agtcctgatc
    20341 gacgcgagcg cgaatgtcct cgtctcggga acgaattcgg aagggccggc ctcattccag
    20401 accgtgacga agctgagggg cacgacaggg gtcccgttgt ggggtccgtg gacgtctgcg
    20461 gcctccggat tcttcggagg aaactcagac atggccctgg atgcctccgg aaacgtcgtc
    20521 gtgacggggg gcgggacgtt caggctcgac gcgaacacgg gcgccgccgt gtgggcctcg
    20581 tcgacggtcg cgcagttcgt ccaattcggg ggggatggaa acgtcgtcct cgccgcttcg
    20641 tcgttcgacg attctctctt cgactgggct ctgactttcc agaagctgga ttccgcaacg
    20701 ggtcttcccc tgtgggctcc gcttcaccgg ccgcatgcct cctcgctcgc tgccctcgcg
    20761 aatgtggagg gcgaccctgt cttcgtcgcc gcgacgacgg atctcgaaaa ggccactctc
    20821 ctgggaaggt actcggacgg tctgacgatc tcgcccgcaa cctcgtcacc cggcgccgtc
    20881 ctttgcggcc gcccgttctc gttcagcttc catgccgaca acggaacacc tccgatccag
    20941 tggaacctcc tctcggggac actcccttcg ggcctcacgc tcgggtcgga cgggacgctc
    21001 tcgggcacga cctccgacgt cggggagttt ccggtgatcg tccgcatcac cgatgggacc
    21061 ggtcgaacga cgactcgcga ggtcaccgtt cgagtgctgg ccgcggcggc cgtcgcggcg
    21121 atccacgtcg cgacgagccc ggcgtgtcag gggccggacc gagtgctctc cgtcatcggg
    21181 tcctacgacg cctacgagtg gctgcccggg ggagagacga ctccgtcgat catcgtgaga
    21241 cccgttgaac gtacgaccta cggcgtgaca gtccgtaccg gttcctgcct ggccaatggc
    21301 gccgtcatcc tcgagcctgg gcccatgctg ctcactccga caattgcggc tccggagaga
    21361 gctcttgcgg gaagcccggg cctcaccgct tccgccacgg agcggccggg cagtacctac
    21421 ttctggtcga tctcgaacgg cacgatcgag tccgggcagg ggacgtctcg gatccagttc
    21481 actgcgggtg tttccgggcc gctctccctc tcggtctccg aaaccgacgc ggctggctgc
    21541 cttcaccctg aggcgtcgcg gcgggtcatg gtcgtctcct cggcggcagg gctgcttttc
    21601 tacgcggttc cgccatgtcg acttctggac acgcggcttc cgtcgccgaa tcccgtgacg
    21661 ctgcggctcg atttcggtta cggggttttc ctggcgggct cctgcgggat tccgttcgcg
    21721 gcacagtccg tggtcatcaa cgtcacggtg acggcgccga cggcagcggg cgacttgcgc
    21781 atcgcggccg gccggccgtc agctccaccg gacgtcagcc tcttgagttt ccagcccggg
    21841 cagacgcgcg ccgtgtccac cctcgtctcc ctgccttccg acggatcgaa cctcgtgacg
    21901 ctctgggggg ccgggtcccc gacggttcat gtggtgctcg acgtctccgg ctattttcag
    21961 taaccggagc caggtccggc ctaggaacca ggtccgtctc agaggaggaa cagccggtac
    22021 gccgggttct tcgtctcgtc cgtgtggcgg tagccgagct cggagaggaa gcggcggaac
    22081 ttcgcgtcgt ccttcctggg cacctggatg cccgcgagga cgcggccgta gtccgcgccg
    22141 tggtttctgt agtggaagag gctgatgttc cagtcgctcc ggaggtgatt caggaagttc
    22201 aggagcgcgc cgggccgctc ggggaactcg aaccggaaga ggcgctcgtc gtgcgccgcg
    22261 ggcgcgcgtc cgccgacgag gtgccgcacg tggagcttcg ccatctcgtt ctcggtcagg
    22321 tcgaggacgg ggaggcgccg cttcttcagc gcgcctacga gcgccgccgt ctgcctcggg
    22381 ctctcgacct cgacgcccac gaagacgtgc gcgtccgtcg cgtccgcgta gcgatagttg
    22441 aactccgtga tactgcgccg cccgacgatc gcgcagaact cgcggaacga gccgggccgc
    22501 tcggggatcg tgacggcgag gatcgcctcg cgcctctcgc cgagctgcgc ccgctcggcg
    22561 acgaagcgca ggcggtcgaa gttcatgttc gcgccgcacg cgacggcgac gagtgttttg
    22621 cccttcgcct tccgcttctc cgcccacgcc ttgagccccg cgacggcgag cgcgcccgcg
    22681 ggctcgagga tcgagcgcgt gtcctcgaag acgtccttga tcgctgcgca gatcgcgtcc
    22741 gtatcgacgc gcacgacctc gtccagcagg tcgcggcaga ggcggaacgt ctccttgccg
    22801 acctgcttca cggcgacgcc gtccgcgaag aggccgacgc gcgcgaggcg cacgcggcgg
    22861 ccagcgcgca gggaccgcca catcgcgtcc gagtccaggg gctcgacgcc gacgatccgg
    22921 gtcttcgggc tgatccgctt cacgtaagtc gcgacgcccg ccgcgaggcc gccgccgccg
    22981 atcgcgacga agatcgcgtc gacgcccgcg ggccgctgcc tcaggatctc catcccgatc
    23041 gtgccctgcc ccgcgatgac gtccggatcg tcgtacgggt gaacgaacgt gcgcttctcg
    23101 gcccgggcga tgcgccgcgc ctccgcgtac gcctcgtcgt acgagtcgcc cgtcaggacg
    23161 acgcgcgcgc cgcggctctt cacggcgtcc accttgatgc gcggcgtcgt gaccggcatg
    23221 acgatcgtcg ccggacaccc gaggcgctgc gccgcgagcg cgacgccctg cgcgtggttg
    23281 ccggccgagg ccgcgatcac gccgcggcgg agagcggcgg gcgagagcct cgacatccgg
    23341 ttgtaggcgc cgcgcagctt gaacgaatgg accggctgca gatcctcccg cttgagcagc
    23401 accgtgtggc ccgtgcggcg cgagaggagc ggggcggcgt cgagcggcgt ctccaccgcg
    23461 acgtcgtaga cgcgcgcgga gaggatccgc tcgaggtact cctctcccgt cttcgcgcgt
    23521 ttcacggacg gacctgccgc gtcagcggcg ccggccgcgg acgagccgga ccggagcggg
    23581 atagcgccgc gcctcgcgga cgggcgtgca ccactccttc gagcgcgcgt gcgccccacg
    23641 cacggcggcg tggtacgcgt tctggagcgc gagcgtgacg gggcccgcgg tccccgagcc
    23701 gatcgtccgg aagtcgatct cgcgcagggc gacgacctcg gccgccgtcc cgcaaacgaa
    23761 gacctcgtcc gccatgtaga gctggtcgcg ggcgatcgtc tgctcgacga cgtcgaatcc
    23821 gagctccttc gcgagggtca agagcgtgtc gcgcgtgatc ccctcgagga tgggggcgga
    23881 cggcgtcgtg acgatgcggt cgtccttgac gacgaagagg ttcgcccccg tgcactcggc
    23941 gacgtagccg gcggggtcga gcatgattgc ctcgtcgaac ccgaggcgca cggactcggt
    24001 cttcgcgagg aagctgttca cgtagttgcc gctgatcttc gccttcgtca tcgagacgtt
    24061 cgggtggtgg cgcgtgtacg acgacacgtt cgcgcgcacg cccctctcga gcgccgcctc
    24121 gccgaggtag ttcttccact cccacgccgc gatgccgatc gcgggccggc cgccgtcgag
    24181 gttcaggttc cagccgccgg cgtccagcca gatcatcggg cggatgtagc actcggagaa
    24241 cccgttcttc gcgacggtcc ccttcgtcgc ctcgatcagc tcctcctccg agaagggaag
    24301 gctgtcgaag ccgagcgcct tcgcggagag gcgcaggcgg cggatgtgct cgccgaggcg
    24361 gaacacggcc gggccggact tcgtctggta gcagcggatc ccctcgaaga cgccgatccc
    24421 gtagtggagg ccgggcgtga ggaaatgcag cgtcgcgtcc tcgtagcgga cgaaatcgcc
    24481 gtccttccag atccatttcg attccattcc catcacaccc tcacttcgga gcgccgcacg
    24541 ccgcttcgcg gcccggagcc atctgtgtcg tcgcggcggc gacctcgtcg gcgatcgccc
    24601 gcgtcatctc gcgcgtcccg agggcgcgcg tgcccttctg cgccaggtcc gccgtgcgcg
    24661 cgcccctctc gagcgccgcc tcgatcgccc ggtgcaccgc ggccgcttct tcgtgcagcc
    24721 cgaggccgtc gccgagaagc atcgcggccg tcgcgatcgc cccgatggga ttcgcgaggt
    24781 cgcgcccggc gatctgcggc gccgagccgt gcacgggctc gaagaggccc ggcccgtcgc
    24841 cgaggctcgc ggacggcagg aggccgagcg agcccacgag cgcgccgcac tcgtccgaca
    24901 ggatgtcccc gaagaggttc tccgtgacga ggacgtcgaa cgccgacggg aagagaacga
    24961 ggttcatcgc gcacgagtcc acgagctggt gctcgagcgt cacgtccggg tgccgcttcc
    25021 cgacttccgt cgtgacggcg cgccagagcc gcgacgtctc gaggacgttc gacttgtcga
    25081 cggacgtgac cttgttccgg cgcgtcgccg cgaggcggaa cgccatctcg gcgatgcgct
    25141 cgatctcggg acgcgtgtac gggagcgtgt tcacggccgt gcccgccgcg aggtcgatcg
    25201 agcgcggcgt gccgaagtag aggccgcccg tcagctcgcg cacgacgacg aggtcgaggc
    25261 cgcgcgcgac ctcgggcttg agcgggcccg agtccgcgag cgccttgtag agccgcgcgg
    25321 ggcggatgtt cgcgtagacg ccgagggcct tgcgcagctg gaggaggccg tactcggggc
    25381 gctggtcgta cggggcgagg tcgtaagccg ggtcgcccac ggcgcccatg aggacggcgt
    25441 cggcgtcgcg gcacgcgacg agcgtgtcct ccggcagggg cgtccccttc tcgcgcagcg
    25501 cgcgcgcgcc gagatgccac tccgagaaga cgaacgtgtg cccgaagcgg gcctcgaccg
    25561 ccttcaggac ggcgacggct cccgccgtca cctccgggcc gatgccgtcg ccgggcagga
    25621 ccgcgatgcg cgcgcgcttt cccattcaga ctccgtagcc gtagccggct tcctcgacgg
    25681 ccgggctctc gtcgacgcgg agctccgggg ccgcaggccg cgccccgttg ccgtggccgt
    25741 tgccgccgtg gtgcgccgga cgcagcgcga cgacgcgctt catcccggac cagagcgaga
    25801 cgagatcccg gtcgtgaacc actttctgac gatccgccag cgcgatcatg cgcctgtaga
    25861 ggtcgtcgag ctcgtggcgg gccgggacga tgccgagcgc ctcgcaccgc gccttcagcg
    25921 cgtgacggcc ggagtgcttg ccgaggacca tcgtcgtttt cgggacgccg acgtcctcgg
    25981 gccgcatgat ctcgtacgtc cggcggtcct tgaggatgcc gtcctggtgg atgccggcct
    26041 cgtgcgcgaa cgcgttgcgg ccgacgatcg ccttgttcgc ctggacggcc tcgcccgtga
    26101 gctccgtgag gagctggctc gccgcgtaga tctggcccgg aacgatgccc gtcgtgaacg
    26161 gcagcttgtc gggccgcacg cggagcgtca tgacgatctc ttcgagcgac gcgttgcccg
    26221 cgcgctcgcc gacgccgttc acggtgcact cgatctggcg gcagcctgcc tcgatggccg
    26281 cgaggctgtt cgcgacggcg aggccgaggt cgttgtggca gtgcgcggag aagacgacgc
    26341 ggtcggagcc ggggacgcgc gcgcgaatcg tcgtgaagaa cctgtagatc tcgtcgggcg
    26401 tcgagtagcc gacggtgtcc gggaggttca cggtcgtcgc gccggcccgg atcaccgcgg
    26461 ccacgaccgc gcagaggtag tcgacgtcgc tcctcgtcgc gtcctcggcc gagaactcca
    26521 cgtcgtcgac gtgccgccgc gcgcgggcga cggcgcgggc cgccgcctcg agggcctggc
    26581 cgcgcgacat gtggagcttg tgggcgaggt ggaggtccga cgtcgcgatg aacgtgtgga
    26641 gacgcggccg cgcgcagccg tcgagcgcct ggagcgcgcg gtccacgtcg gcgtccacgg
    26701 cccgcgcgag ggcggccacc gtcggacggc ggaccgcctt cgcgac
//
LOCUS       DAOMFY010000024        25810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_619_length_25810_cov_9.443681, whole genome shotgun sequence.
ACCESSION   DAOMFY010000024 DAOMFY010000000
VERSION     DAOMFY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25810
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_619_length_25810_cov_9.443681"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..226
                     /locus_tag="PLB01_03810"
     tRNA            152..226
                     /locus_tag="PLB01_03810"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:184..186,aa:Asn,seq:gtt)
     gene            complement(212..1855)
                     /locus_tag="PLB01_03815"
     CDS             complement(212..1855)
                     /locus_tag="PLB01_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQQ76457.1"
                     /translation="MTTNGFTPEKRCGLVVRVSTDRQAQNDEGSLVTQKQRLRERIES
                     KRKQGENWREAEIYELRAVSGSTSLNNPEMHRLRADIQARRVNVVLATDLSRISRSLP
                     DFLQFFTFATGHGAEVVCLHPDLDSTTPSGKAMTRIIMVFAELERDQVCQRNRESTFA
                     RAQRGLWNGGHIFGYDLPEGKKGTLVVNERQAEIVRLAFSIYETTGSILETSKELTAR
                     GFRTEAYTSRRDRSHASVAFGYTKTRWLLQNKAYVGVREVNKKNKTVDPETLPESQRY
                     REVKANWEPILDRETFDRAQRLLTANTLSARNVAKQVRHVYVFNGGLLYCEACKKQME
                     GRSGTSRMKRPYFYYFCKVCKLRIPAGEVEKVVLFRIRELAKKPSLLTPLVAETNRRL
                     TEELPELESRRKAAVQELGAVAADADATFRHAAALDASAASVFVREKLEKLAVQRKGL
                     ESTVAELDDAIDKIRKTAVDQEKVVTAINHFRRVYAKLQPYQRKELIRLVVARAEVSE
                     SSLRLQFHGNPASEEVFEKIKAPEGVPLRRFEPVKWLRR"
     gene            complement(1852..2259)
                     /locus_tag="PLB01_03820"
     CDS             complement(1852..2259)
                     /locus_tag="PLB01_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76458.1"
                     /translation="MSSPPKSAGVAVVGEGRHQNGQGLVPPLAEALAREVRAGADVPQ
                     SDGFGTEGTIGTAVPEGEVPLPAGPDPFLHARREAALVELLATGVVAFLKDVTSNDEL
                     PDDDGVLSEDGFACSNFLFEQAEPTVLEQEEDP"
     gene            complement(2288..5047)
                     /locus_tag="PLB01_03825"
     CDS             complement(2288..5047)
                     /locus_tag="PLB01_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HQQ76459.1"
                     /translation="MAPHHRTQLEASAIKQEVWEARGVFSVVDADAARTLGFSQAQSR
                     VPALVFPLFDPTGRASGHQLRPDAPRLRDGKPAKYESLPGRAPVLDVNPLCTARLADP
                     ANRLWITEGVKKGDALVSAGEVAMAINGVYGWRGKNGYGGVAALPDWENVPLNGRDVI
                     LVFDSDLSTNRNVATALMRLAAFLRSRGASVRTVYLPAGADGAKVGVDDYLAAGGALD
                     ALLSEPAPTEEHRELIVFENDDAEPAHPFKVTPNGIVMMKPTKNGGVVEILLTNFTAE
                     IVEDVELDDGVESTRHYRIRAVVGGRERTVSVPAHEYHSLGWIPTKLGARAIVSPGLA
                     AQDCTRAAIQSLSTDSRERTVFAHLGWRERDGELFYLHAGGAITAEGLRTDVDVEVPG
                     GLERYALPDPESVDLAASFAAVRRLLETLGAVRGWRLLSLVPAAILGEIVPTDFVAWV
                     WGRTGTFKSTAAALVSCFFGKFDRLSLPGNFTSTANATQGIAHAAKDVVFVVDDFAPA
                     ANQSLQSVQDQTVFKLIRAVGDRHGRNRMRPDRTLVGGKPPRGLVLVTAELPQPAGQS
                     TQARVFEIPWDNVGINNEALAWAQQEALLYAGVTAAFVKDVASRFAVLREELPRRVEA
                     LRSEFVASHRRVAETASKLVAAVEVFLDFAHRSGLVSEAEKASLRGAAVEAIAEAARG
                     SSEAEMERSPITLLLEGLRAAFSLGQAYLDHVVGGPPLVDREKWGRVHGEPGPQSALI
                     GWRDENRGLVYLVPSAALLAASRALGGDIALPRSVAAVGALLRAERLLHHAEPGRSTI
                     LMRIGQTRPRVWTLREDHFVPKDEPEEAQPSPAWDIASVPLPDDDLVLEVEPAKAKRK
                     KPVAKPPAAPAAAVPPTTVPPVTADGQTKTAATPAADAPPVLEVPETPTDAKSEGVPD
                     DRKER"
     gene            complement(5506..5985)
                     /locus_tag="PLB01_03830"
     CDS             complement(5506..5985)
                     /locus_tag="PLB01_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76460.1"
                     /translation="MRTEDVVQAARHALGYWDSSSDEAIANRYEEQVTRAWRNALERQ
                     FPGRLNFEVEPVSGEHRSRIDAVDFVEGVAYEFKVSGKNPHHRFFRDLWKVIALNAQD
                     GVTVRRLVFVVGKPGDRELSDAFTTRVREVAAQTLGIETEIVGVLPPHRPSPFDNDD"
     gene            6777..7421
                     /locus_tag="PLB01_03835"
     CDS             6777..7421
                     /locus_tag="PLB01_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76461.1"
                     /translation="MDKKERQLAAGLVARLCSGDFGESDVLALLIVLRRHAQGTAPFV
                     AELGDFVAHREKDRGLLLKFLQAVQASITGTGPKRDEFEVPGSAVALHQELERLLPAL
                     DLPTMPLETVECLVVLASSLLQSVAFDAAVEQPWQEWRLEFGFTRDVVGLYGVVPLPK
                     SSMLFPVLLARNRFFSSPYEGGEACWTPDRSFFVTTWCRGGIVFFDQQPTTPRR"
     gene            7502..8131
                     /locus_tag="PLB01_03840"
     CDS             7502..8131
                     /locus_tag="PLB01_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76462.1"
                     /translation="MNDSSFYEFFGHAVDADIQLALLREYSTAIHAFLKKAAEPDSDL
                     PDALQAIATDRAYLYEEVFPQVLHESFLITCVVFLERELRTIVEILRRITGTPLTFGD
                     LSGSNAERFRTFCTKVCAFDPGLSNEQWQALTGLIEVRNCLVHSSGLLEDFPKRAAVE
                     TFVRRSPDFKIEDGRLVVSEATSARAVAILSDFVETVFRAGTEKYPREA"
     gene            8186..8629
                     /locus_tag="PLB01_03845"
     CDS             8186..8629
                     /locus_tag="PLB01_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76463.1"
                     /translation="MASTTFHPRAFPTLHRYFMWSVLMKDRFEEALSEPEGRQGDGFA
                     YLATKAGTFMSYWYGGLYVVVEGWRELGLSDPVIDPLLTSPNVEALRLFRNATFHYQH
                     QWLHQKHRAFMQIEGSVSWVRQLHAAFSQYFLREMRARYRSHRDT"
     gene            8817..9119
                     /locus_tag="PLB01_03850"
     CDS             8817..9119
                     /locus_tag="PLB01_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76464.1"
                     /translation="MLRRLLVTLCAVVLMQETNLGSLIVGAECFEKCDDDIGPGHCSP
                     ACATCVCGTHAKPHAPGATRLPAPAVTTTRELPRVARATGDLHLPDILHVPKRFVA"
     gene            9261..10478
                     /locus_tag="PLB01_03855"
     CDS             9261..10478
                     /locus_tag="PLB01_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQQ76465.1"
                     /translation="MVAVLGGASALRADEPLRIETYVEIVLQSHPAAQVARAFDESAA
                     AESRVARLFPDPTLELSLGQGRSVTDSSVRATETGITLSQVIPWFPARANAIDAADFA
                     ADARRAEGSAARWRLRIDARLAFYRLLQARALVDVARATETDARSLLDLVNRRASLGE
                     TREVDRIKARVEWLKQQRELHKAEREAEAAEAALRTLAATPLSRPLVLNGELPKPADS
                     TSTLEDATPARLDRSPEMARARAEAARAVSLLSNARRSRVPDLGLSLFSLKELDRDAY
                     GASLGVVIPLWNARRGDIARAGAESMLRSADTDRIRLALLTEVETRRRDVDTSAAEAA
                     TLMNELLPSAAESLRLARLLFEEGETSLLDLLDAQRTARDARREEIRARFELAVALSE
                     LQRLLGPEETSGR"
     gene            10485..11717
                     /locus_tag="PLB01_03860"
     CDS             10485..11717
                     /locus_tag="PLB01_03860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ76466.1"
                     /translation="MNTRTLHQLTVVTLCLALALGGCSKRKGAPAGDSEKKTGEASQE
                     PAVPHQVRLTPAAIAEAGITTWKVQVVDLSHLLSLTGSVGYDENRLLQVAANVKGRVA
                     KIPVDLGARVKAGDPLLVIESVDLGRAREEFVKELSSFNVSANAYERAKKLVAANAIS
                     SGEFQTREGDYLSRKAAVASAERTLHLYGDSDDAVARLRAGAGGQTLSAADGATLTLR
                     APFAGRVIDRKVTPGALFEALQPLMTLADLGSVWVFLNAYEKDLALLHDGLPVTLRTD
                     AYPQESFHGRVDFLGSVVDPQTRSVRVRATVENRAEKLRPGLFVKAQVEVPRPQSESH
                     QIVAIPQGALQTLEGRATVFVQTEPGLFVRRLVETGHTFEGFTEVLSGVKPGDVVVTE
                     GSFVLKSEFAKASLTEKD"
     gene            11719..14808
                     /locus_tag="PLB01_03865"
     CDS             11719..14808
                     /locus_tag="PLB01_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQQ76467.1"
                     /translation="MLRRLVDFSLENRPLVLLAAILVIVGGAYALTQLPIDAVPDITN
                     VQVQVLTKAPALGPVEMEQFVTYPVEAAMNGLPKLREIRSISRYGLSAVTVVFEDGVD
                     VYFARQLVAERLVQAREAIPPNQGNPEMGPVTTGLGDVFQFTVEGDGVSAMERRTILD
                     WTIAPRLRAVPGVTEVNTWGGLPKQYQVVIDPAKLRAHGLSLKEVFEAVGRGNANAGG
                     GYIEHNREQYIVRGEGLVGSLADIERIVLKSGPEGTPVTVGAVAQVREGAMLRIGVAT
                     ADGRGETVIGLVQMLAGENALEVATRARKAVEELQPTLPKGIRVVPYYDRATFVRRVI
                     RTVETNLLEGSILVIAVLFAFLGNVRAGLIVASAIPLSMLLAFTGMVESRISANLMSL
                     GAIDFGLIVDGAVVLVENVVRRLSEPEGQSKTVRQLTAEAAHEVVRPITFGIGIIALV
                     YLPILSLEGVEGKMFKPMAWTVVFALAGSLLLTLTLTPVLASIFLKKTGHEHEPRFVG
                     KLRALYLRGLDICLARRAPVILGAILAIAIGAVVAARLGGEFLPRLDEGDLSISAIRP
                     ASVGISEVAASTGRIERVLKKFPEVITVVSRSGSPELATDVMGIEQGDVFVILKPKGE
                     WTTARTKGELVEKMEHALDEAVPGVGFSFLQPIEMRFNELIAGVRSDVGVKLFGDDLE
                     TLRQKGEEIARVVAGVRGAADVKVEQTAGLPVLRVKVDRERCARYGISVGDVLDTVEA
                     ARAGKIVGTVFEGQRRFSLAVRFDDETARTIEGLGNVPVATPGGALVPLGQLADISLE
                     TGPAQISREAVKRRIVVELNVRDRDVASFVAEAKAKLASGVKLPDGYYIRWGGQFENL
                     DRASRRLTVVVPLALALIFAMLYFTFGALKPAALIYLNVPLAATGGVFALALRGLPFS
                     IAAAVGFIALFGVAVLNGVVLMTQIRDLEARSDMGVADVLRKACGLRLRPVLMTALVA
                     SLGFVPMALSTGSGAEVQRPLATVVIGGLITSTILTLFILPTIYSLFGRRNAGPTPA"
     gene            14817..15185
                     /locus_tag="PLB01_03870"
     CDS             14817..15185
                     /locus_tag="PLB01_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQQ76468.1"
                     /translation="MDYDIINTLVAYIRPFQLDAVVDALRKLPNFPGLSVSDVRGFGH
                     HGAHPPHRGETAEVDPFTPMARLEITCRGQELSGIVEAIRQAARTGHHGDGKIFINTT
                     AWACRIRTDEEGPDAVLGGP"
     gene            15194..17110
                     /locus_tag="PLB01_03875"
     CDS             15194..17110
                     /locus_tag="PLB01_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQQ76469.1"
                     /translation="MSFLSTLVRAPEMRSVLAGGALLAAGLAIDHLGVDHGVAVAIYV
                     ASMLVAGWHWMREGWRELVHCREIGIEVLMFAAAVGAAALGLWDEAAALVFLYGLAEA
                     IEEQSYDRARRAVERLLDLVPAQATVVRDGTEILVEASRLQREDRIRVRPGESIPTDG
                     VVEEGDSAVDESSLTGESIPVEKKPGDRVFAGTLNRSGSLVFSATAAAEENSVSRLVK
                     LVEAAQKSKGRSQQVVERFTNVYSPVILGVAVLLLLLPFVIGGSFSVWARHAVVLLVA
                     AAPCALAMSTPVAFAAGMSAAGRHGILIKGGRYLEVLGRVRTVAFDKTGTLTRGRPEL
                     VVAEPFAGTTEEVLLSTAASLERFSEHPLAAAVLRAAEVKGVTNLRAASGFRALPGAG
                     VEGVVDGARVQILQPDGGGESFGGLGQRLEDLARAGITPAVVLAGGRPIGVLGFRDEP
                     RPEAPAALRHLRAAGITRLVVLSGDRPLVARSVAQALGVDDARGGLQPEHKVDAVRTL
                     SLECGPLAMVGDGINDAPALAAADVGIAMGALGSDASIEAADVALMADSLDHLDEAFL
                     IGQRVRSIVRQNVIFSIAVLAILIPVALSGLISITVTVVAHEAAELIAVANGLRVLGS
                     GRNHERDEGRRLNR"
     gene            17313..18200
                     /locus_tag="PLB01_03880"
     CDS             17313..18200
                     /locus_tag="PLB01_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76470.1"
                     /translation="MSTVKRFVPGVLLFALLGLALPAIAQTGGMQNGPFDGMSAGIVV
                     PAAVRADGKNGAFFQTDLWIRCRTVPVAATLYFHAADAASATPTVTVPVTLTQPVTYL
                     PDILSATFGVSKGAGNIRIVAAAPVSAVLRVYNNGGGGAFGLSFMGMPSTMSLQSMPM
                     MGGSFGMDDFAMYMLGLLPEPGNRVNVSVVNTNSTTVNGVVEVLDSDGLAPTGTGVTS
                     LPFSIAGYSSHQFNDVLLNVHSRFGSGDTGLQIRVRLNQGSAGMVMSYAVVNDNTTND
                     GYVVMGNMMNGGRGMGMMN"
     gene            18593..20467
                     /locus_tag="PLB01_03885"
     CDS             18593..20467
                     /locus_tag="PLB01_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQQ76471.1"
                     /translation="MPSPDRARSSAPAAWLIVLLSAQLRAADPSPPAYSATDIKTTIV
                     ENLPPPIPNAYHALGDYIFAVQPLRGNFHGDFNGDGLEDILVAPNYATFQPQLPIQIW
                     INQGNGTFVDQTSTVIEGPITVTGAATSIFVADFNGDGRPDVFFVDSGLEDKDPSAGF
                     DGGINHVLLSQPNGKLKDVTSDSLPQNVFRFNHVSSIGDVNGDGTLDIVLTVLGGRTG
                     GAGTLFLLNDGRGVFTSTSSGLPLDIAYYPSGPWWPAAPDPQLPGSNGVLDLDGDGRL
                     DLVTGTYSLNDPVSKKRSVRFFQQRPDHSFVERSRIEIPLALANIGYRGGTVADSGGA
                     GLGVAGITGADLDEDGRTDVVVLWEGASASYIQILRNEGNFVFTDKTLDWLGTYDTNV
                     VAGGLKWSASAYDLMDVNGDGHIDLVRRGAGYDAATLWNGGFAFLNDGTGHLKPLAYR
                     TRSSAAGLDDFIGALGCSYCAYTPFLFDATGDGQIDLVLLDISSGNSGRWTPEKPFRD
                     KELYLHVFSGQGSLAGLSASFSYGPSSPAAGTPVGFTDTSSGATSREWKFGDGGTSTS
                     AQPLHTYSAPGLYTVQMTARNGTQAMTASRRIAVTLGSTTRHQPVSGPPGGQPVKKRP
                     "
     gene            complement(20761..21234)
                     /locus_tag="PLB01_03890"
     CDS             complement(20761..21234)
                     /locus_tag="PLB01_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76472.1"
                     /translation="MGAPALVLAILIASGPPCTPPARPPDTMLDWLSNAPLRAALGRL
                     FARAEYGLASYESAAWIVRERGGDVVLRDWSFTGAVERAAWHGPPPDGALAIVHTHPD
                     RADPRPSSVDAALARRLSLPVYTLTRSGVWVVRADGTVLLEAPAPRACARIACLP"
     gene            complement(21304..22848)
                     /locus_tag="PLB01_03895"
     CDS             complement(21304..22848)
                     /locus_tag="PLB01_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQQ76473.1"
                     /translation="MARWEFPIPVDRASELPVYLQIVRSLAQDIRRGRLLPGDRLPGS
                     RALAHALGLNRNTVVAAYRELEAEGWTTGSEASGTFVSGTCPCEVPCSFGVLRRKVPS
                     RVGFELVPSPSVSPGPLFRPGNLDFGNGSADVRLVPVEALARAYRTALRMDGHRVLDY
                     QTRGRSALELENGAYGSRDLRQALAQMLAATRGLAVGWENVIVTRGSQMGLHLAARAL
                     LGPGDVVAVEELGSKPVREALRQAGASVVPIPVDADGIDVEAVRALVDREPAVRAVYL
                     TPHHQCPTTVTLVPGRRLQLLELARRHRIAILEGDYDHEFHYESRPVLPMASVDTAGV
                     VVYVGTLSKILAPGLRIGYVVAPRPFVDRLAILRQVSDIQGDLAGEVAVARLLAEGDV
                     ARHARRVRRLYRNRRDLLAKALKKELGDALSFAMPTGGMGIWAQAAEDLDVERWSALA
                     VEEGVSVRTAQTYAVDDQPRPFLSIGFSRLDETELVEAVRRLARARRRLVATSSFAPA
                     RAATGG"
     gene            22943..23878
                     /locus_tag="PLB01_03900"
     CDS             22943..23878
                     /locus_tag="PLB01_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQQ76474.1"
                     /translation="MKDRPGPAEGTLELDQIRAFVFAVEEGSVQGAARRLHRSQPAVS
                     RLIQSLERALGESVLDRSTRPASPTEFGREVLDEARRALDAVDRLEGRGRTDAGAKRT
                     TIRLGVSHAYTRIVASPLVSVALRASGNPALRIHTGWSIPLLKELLESRLDAALMGFP
                     GRWSPPADLRSEPVREEELVVIAPRDTKIRGITRLRDLAPHPWVLNSEGCGLRALLQR
                     EFAESGRSLDLHLELQGSLSQHIEFVAAGFGFGLVPLCALTIHPQRREVRRVPLKETL
                     ATNLWILWRPSDRAADARWAGIRAALVRSATPRAL"
     gene            complement(23897..24715)
                     /locus_tag="PLB01_03905"
     CDS             complement(23897..24715)
                     /locus_tag="PLB01_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HQQ76475.1"
                     /translation="MTEAATPNRATAFLAMHRPGAGFILPNAWDGGSARILEQAGFAA
                     IATTSAGIAFSRGAADGTMERAEMLDCIARIVSAVACPVSADLESGYGATPGHVAATV
                     AAAVELGVVGGNLEDVIQPGQLLPVEAACERLEAARSAAPSGSFVLNARTDAYMLRRP
                     DAFAETVRRAERYLAAGADCIFVPGVSASEEIGRLTAAIGAPVNVVAGLVEPVLDAAT
                     LRSLGVARISVGGTLARAALTRIEQAAREMADHGTFGFAHGAIPYAELQRRFGH"
     gene            24903..>25810
                     /locus_tag="PLB01_03910"
     CDS             24903..>25810
                     /locus_tag="PLB01_03910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ76476.1"
                     /translation="MTLEPGTRLGPYEVSSPLGAGGMGEVYRARDPRLARDVAIKVLP
                     EEFFEGEERRVRFEREARTLASLNHPGIAAIYSFEEIPSSSSSSSATRHILVMELVDG
                     EDLAQRLGRGAIPVDEALAIARQIAEGLEAAHEKGIVHRDLKPANVKLTRDGVVKILD
                     FGLAKAFEGDAASSSSSPSVTQSPTMSRPLTEAGMILGTAAYMSPEQARGRPVDKRTD
                     IWSFGVVLHEMLTGKRLFAGETVSDTLAAVLRQDLDLAALPAETPDRVRRLLARCLVR
                     DPKLRLRDIGDARLELDAGGADRPADV"
BASE COUNT         4119 a         8615 c         8639 g         4437 t
ORIGIN      
        1 ccaaggggac atttctatcg agttatcgag gggacacttt catggagcta cgacagctga
       61 atcccgaagg cttgacgcgg agaggcgcga accgtacgat gcgcggcctt cgcgcggccc
      121 gcgcgtgcgg agagcccccg ctctttcaca ttccgcagta gctcaatggt agagcactcg
      181 gctgttaacc gagatgttgt aggttcgagt cctacctgcg gagccacttc actggctcaa
      241 agcgtcggag gggtacccct tccggcgctt tgattttttc gaagacctcc tccgacgcgg
      301 ggttgccgtg gaactggaga cggaggctcg actccgagac ctctgccctc gcgaccacga
      361 gccggatcag ctccttgcgc tggtagggct gcagcttggc gtagaccctc cggaagtggt
      421 tgatcgccgt gacgaccttc tcctggtcca cggcagtctt ccggatcttg tcgatggcgt
      481 cgtcgagttc ggcgaccgtg gactcgaggc ctttccgttg caccgcgagc ttctcgagct
      541 tctccctgac gaagaccgag gccgcggagg cgtcgagtgc ggcggcgtgc cggaaggtcg
      601 cgtcggcgtc cgcggccacg gcgccgagtt cctgcaccgc ggccttcctc cgggactcca
      661 actccggcaa ctcctcggtt aggcggcggt tggtctcggc gacaagaggc gtgaggagcg
      721 agggtttctt cgccagctcc cggatccgga acagcacgac cttctccacc tcgcccgcgg
      781 ggatcctgag cttgcagacc ttgcagaagt agtagaagta gggcctcttc atccgcgacg
      841 tccccgaccg cccctccatc tgcttcttgc aggcctcgca gtagaggagc ccgccgttga
      901 acacgtacac gtgacggacc tgcttcgcga cgttccgggc cgagagcgtg ttggcggtca
      961 ggagcctctg cgcacggtcg aacgtctcgc ggtcgaggat cggctcccag ttcgccttga
     1021 cctcgcggta ccgctggctc tcggggagcg tctcgggatc gacggtcttg ttcttcttgt
     1081 tgacctcgcg cacgccgacg tacgccttgt tctggaggag ccagcgggtc ttcgtgtacc
     1141 cgaacgcaac gctcgcgtgg gacctgtcgc gccgcgacgt gtacgcctcc gtgcggaagc
     1201 cacgcgcagt cagctccttg gaggtttcga ggatggagcc ggtcgtctca tagatggaga
     1261 acgccagccg cacgatctcg gcctgccttt cgttcacgac gagggtgccc ttcttcccct
     1321 cgggcaagtc gtagccaaag atgtgtccgc cgttccagag cccccgttgc gcccgagcga
     1381 aggtggactc gcggttgcgc tggcacacct gatccctctc gagttcggcg aagaccatga
     1441 tgatcctcgt cattgccttg ccggacggcg tcgtcgagtc gaggtcagga tggaggcaga
     1501 ccacctcggc gccgtgcccg gtggcgaatg tgaagaactg caggaagtcc ggaagggagc
     1561 ggctgatccg ggacaggtcc gtcgcgagaa caacgttcac gcgcctagcc tggatgtcgg
     1621 ctcggagccg gtgcatctcc gggttgttga gcgacgtgct accagagacg gcgcgcagct
     1681 cgtagatctc ggcctctcgc cagttttcgc cctgcttgcg cttggactcg atccgctccc
     1741 tgaggcgctg cttctgggtg accagcgagc cttcgtcgtt ctgggcctgc cggtcggtgg
     1801 agacgcggac cacgagcccg cacctcttct cgggcgtgaa gccgttcgtc gtcacgggtc
     1861 ctcttcctgt tcgagaactg tgggttcggc ctgctcgaac agaaaattcg aacatgcaaa
     1921 gccgtcctcc gacaagacac cgtcgtcgtc cggtagctcg tcgttggacg tgacgtcttt
     1981 caagaacgcc acaacgcccg tcgccagcag ctcgaccagc gcggcctcgc gccgggcgtg
     2041 caagaagggg tcggggcccg cggggagggg aacctccccc tctgggaccg cggtgccaat
     2101 ggtgccttcg gtaccgaacc cgtcgctctg cggcacgtcc gcgcccgcgc gcacctcgcg
     2161 cgccaaggcc tccgcaagag gcggcaccag accttgtccg ttctggtgcc gcccctcgcc
     2221 gacgactgcc acgcctgcgc tcttgggcgg cgacgacacg tcgggcctcc gtccgtgacc
     2281 tctctcgcta gcgctccttc ctgtcgtccg gcacgccctc cgacttcgcg tcggtcggcg
     2341 tctctgggac ctccagcacc ggcggggcgt ccgcggccgg agtcgcggct gtcttcgtct
     2401 ggccgtccgc cgtcacgggc ggtacggtcg tgggcggcac cgccgccgcc ggcgcggccg
     2461 gcggtttcgc aacgggcttc ttgcgcttcg ccttcgcggg ctccacctcg agcacgaggt
     2521 cgtcgtcggg caggggaacg ctcgcgatgt cccacgcggg gctcggctga gcctcctcag
     2581 gctcgtcctt cgggacgaag tggtcctcgc gcagcgtcca gacccgaggc ctcgtctgac
     2641 cgatccgcat taggatcgtc gagcgaccgg gctcggcgtg gtgaagcagg cgttcggcgc
     2701 gaaggagagc gccgacggcc gcgacgctac ggggtagcgc gatgtcgccg ccaagggctc
     2761 gcgacgccgc gaggagggcc gccgagggaa cgaggtagac cagaccgcgg ttctcgtcgc
     2821 gccacccgat cagagccgac tggggccctg gctccccatg gacacgcccc cacttttccc
     2881 tgtcaaccaa gggggggcct ccgaccacgt ggtcgagata ggcctgcccc aagctgaagg
     2941 cggcgcgaag cccctccaag agcaacgtga tgggactccg ctccatctcg gcctcgctcg
     3001 agcccctcgc ggcctccgca atcgcctcga cggcggcgcc cctgagcgac gccttctccg
     3061 cctccgagac caagccggac ctgtgcgcga agtcgaggaa gacctccacc gccgcgacga
     3121 gcttcgacgc ggtctcggcg acccggcggt gcgacgcgac gaactccgac ctgagcgctt
     3181 cgacgcggcg cggcagctcc tcgcgaagga cggcgaagcg gctcgccacg tccttgacga
     3241 aggccgcggt gacacccgcg tagaggagcg cctcctgctg cgcccacgca agcgcttcgt
     3301 tgttgatgcc gacgttgtcc cacgggatct caaagactcg ggcctgcgtg gactggccgg
     3361 ccggctgcgg aagctccgcc gtcacgagca cgaggccgcg gggcggcttg ccgccgacaa
     3421 gggtgcggtc tgggcgcata cggttgcggc cgtgacggtc gccgacagcg cggatcagct
     3481 tgaagacggt ctggtcctgc accgactgca ggctctggtt cgcggccggc gcgaagtcgt
     3541 ctacgacgaa gacgacgtcc ttggcggcgt gcgcgatgcc ctgcgtggcg ttcgccgtgg
     3601 acgtgaagtt cccgggcagt gacagcctgt caaacttccc gaagaagcac gagacgagcg
     3661 ccgccgccgt gctcttgaac gtgccggtcc tcccccagac ccacgcgacg aagtccgtcg
     3721 gtacgatctc gccaagaatg gcagcgggca cgagagagag taggcgccag ccgcggaccg
     3781 cccccagcgt ctccaggagc ctccgtaccg ccgcgaacga ggccgcgagg tcgacggact
     3841 ccgggtcggg cagcgcgtag cgctcgaggc cgccggggac ctcgacgtcc acgtccgtgc
     3901 gcaacccctc cgccgtgatt gcgccccccg cgtggaggta gaacagttcg ccgtcgcgct
     3961 cgcgccaccc gaggtgggca aacactgtcc gctcccggct gtctgtggag agggactgga
     4021 tcgcggctcg cgtgcagtcc tgcgcggcca ggccgggcga cacgatggcc ctcgccccga
     4081 gcttcgtcgg gatccagccg agcgagtggt actcgtgcgc cggcaccgaa acggtccgct
     4141 cgcgtccacc cacgaccgcg cgaatcctgt agtgccgtgt ggactcgacg ccgtcgtcta
     4201 gctccacgtc ctcgacgatc tcggcggtga agttcgtgag aaggatctcg acgacgccgc
     4261 cgttcttggt cggcttcatc atgacgatgc cgtttggcgt gaccttaaac gggtgagcgg
     4321 gctcggcgtc gtcgttctcg aagacgatga gttcgcggtg ctcctcggtg ggcgccggct
     4381 cggaaaggag cgcgtccaac gcgccgcccg ccgccaggta gtcgtcgacg ccgaccttcg
     4441 cgccgtcggc gcctgccggc aggtacacgg tccgcacgct cgcgccgcgg ctcctgagga
     4501 aggcggcgag cctcatgagg gccgtcgcca cgttcctgtt cgttgagagg tcggagtcga
     4561 agacgaggat cacgtcccgc ccgttgagag gaacgttctc ccagtctggg agcgcggcga
     4621 cgccgccgta gccgttcttg ccgcgccagc cgtagacgcc gttgatcgcc atcgcgacct
     4681 cgcccgcgga cacgagcgcg tcgcccttct tgacgccttc ggtgatccag aggcggttcg
     4741 cggggtcggc gagcctcgcc gtgcagaggg ggttcacgtc cagcacgggg gcgcgaccgg
     4801 gcaacgactc gtacttggcg ggcttgccgt ccctgagccg cggggcgtca ggtcgaagtt
     4861 ggtgcccgga ggcgcgtccg gtcgggtcga agagcgggaa cacgagcgcg ggcacgcgcg
     4921 actgcgcctg tgagaacccg agggtcctcg cggcgtctgc gtcgacgacc gagaagacgc
     4981 cccgcgcttc ccagacctcc tgcttgatgg cggaggcttc gagttgcgtg cggtggtgcg
     5041 gcgccacttc actcggcgac gacgagggcg aacacgttga agatggggtt ggggtcgaag
     5101 gttccataca ctgctcctct tttcgaatgc ggagcagtgc ggaaactctc tgcctcccca
     5161 gagcgctacg aagtccggtc tacgaatccc cgcgccgacc ggcggctaga taatcgggga
     5221 ctgcgtggca ggcgtgttta agccccgggt ccgggaggag tgcggccccg gggtcgggcc
     5281 gcacggaaag tatccgcgcg cttctcgatg ggtgtcaaga cgttgacaag tctccttggg
     5341 cgcactacct cggtagccgc cggcgcggcg agaacgacct gacgtagcga agcagtcaag
     5401 gtataccggc accgccggca ccgctgtcac cgcggttggg agaggggagg ggaaaagttt
     5461 gggtgggaga ggaaagtggc gacgtaggcc cgggggaggg aactcctagt cgtcgttgtc
     5521 gaacggcgac ggccggtgtg gaggaagtac cccgacgatc tcggtctcga tgccgagcgt
     5581 ctgtgcggcg acctcgcgga cgcgtgtcgt aaaggcgtcg gacaactcac ggtcgccggg
     5641 cttgccgacg acgaagacga gtcttcgaac tgtcaccccg tcctgtgcgt tgagcgcaat
     5701 gaccttccac aggtcgcgga agaagcgatg atgcgggttc ttgccagaaa ctttgaactc
     5761 gtatgcgacg ccctccacga aatccaccgc gtcgatcctg ctgcggtgct ctccggacac
     5821 tggctccacc tcgaagttga ggcggccggg gaactgtctt tccagcgcgt ttcgccacgc
     5881 ccgggtcact tgctcctcgt agcggttcgc gatggcctcg tccgacgacg agtcccagta
     5941 cccgagcgcg tgacgagcgg cttgtacgac gtcctctgtt ctcatgaggt gcctcccaca
     6001 aggatggtgt cgttgtcgtc ccccttcccg gaacgttcgg cgcacgcgtc tgcagccaat
     6061 ggcgaggttg ccgcgcgagc agggagagac gtcccggccc gacgcgcccc gccccgacca
     6121 catgactcac caccggagat tgatttctgc cgggtcgaag gtgcgcttca cgccgcacgc
     6181 gattcgggtc agactggctt tatcttgtta aagcagcttt gtcacggtcg aggtacatga
     6241 ccccctgggg tccgtctctt cggaccacag cacggggggt tgctactgtc ctctgtttcc
     6301 acggcaccga aggcgacgga ggtgccttcc ctcgcgacga cgacctactt cctcagcgcc
     6361 gctccccgtg ctttgatcgg tgggagcatg gcgaccgacc aagagcagga ccgccgtcga
     6421 atcggacggt ctcttcacct gagcgacaag tggcacgagt tcgcggagta cggcgaggtc
     6481 atggccacgt gctggatccc agaagatcag atcctcgtcc acatcagacc gagctggcgg
     6541 tcgtggcaga tcgctgcggt gatcatccga gccttgactc gggtagcttc agctcggccc
     6601 aagctcctcg tcttcgtcgg cgcatacccg gactcttacc acacggggtg gctgggtcac
     6661 ctgcggtgct tggccgtcca ctgcggggta ggaacgcaca gcacacgccg gtggaaggcg
     6721 aagactggcg gtgggaccaa tgaagtccga agctcctaac taggaaggcg actgagatgg
     6781 acaagaagga gcgacagctc gcggctggac tcgtggcccg actgtgttct ggcgacttcg
     6841 gcgagtcgga cgtgctcgca ctcctcatag tcctgaggcg tcacgcacag ggaacggcgc
     6901 cgttcgtagc ggaactcggt gacttcgtgg cacaccgaga gaaggaccgt gggctcctcc
     6961 tcaagttcct tcaggcggtg caggcttcaa ttacaggtac gggtcccaag cgcgatgagt
     7021 tcgaggtacc tgggagtgca gtggccctcc atcaggagct ggagcgtctg cttccagcgc
     7081 ttgatcttcc tactatgccg ttggagacgg tggagtgcct tgtcgttctg gcgtccagtc
     7141 tgctccagtc cgtggccttc gacgctgccg tggagcagcc gtggcaggag tggcgcctcg
     7201 agttcgggtt tacgagagac gtcgtcggtc tctacggagt ggtgcctctc ccgaagtcct
     7261 caatgctctt tcccgttcta ctagcgcgca atcgcttctt ctcgtccccg tacgagggcg
     7321 gcgaggcgtg ttggacccca gaccggagct tcttcgtcac cacctggtgt cgaggtggaa
     7381 tcgtcttctt cgaccaacag ccgacgacac ctcggcggta gtagcgttgg tcccgccggt
     7441 agttccgaag gcaccgctgt cacagcggag accaaacgct agagcgtgcg aggccaacat
     7501 catgaacgac tcttccttct atgaattctt cggccacgcg gtcgacgccg acatacagct
     7561 ggcacttctg cgggagtatt cgacagcgat tcatgcgttt ctcaagaagg cggctgagcc
     7621 agactctgac cttcccgatg cacttcaagc gatcgcgacc gaccgcgcct atctatacga
     7681 agaagtgttt ccgcaggtcc ttcatgagtc ctttctaatc acctgtgtcg tcttccttga
     7741 acgggagctc cggaccattg tggagatctt gcggcgaatc accggtacgc cgctcacttt
     7801 tggtgaccta tccgggtcca acgccgagcg cttccggacg ttttgcacca aggtctgcgc
     7861 ctttgatcca ggcctttcaa atgagcagtg gcaggcgctg actgggctaa tcgaggtaag
     7921 gaactgcctc gttcactcgt ctggccttct cgaagacttc ccgaagcgcg ccgctgttga
     7981 gactttcgtt cgacggagtc ctgacttcaa gatcgaagat gggcgtctgg tggtgtccga
     8041 ggcaacctct gcgcgtgcgg tggctatcct cagcgacttc gtcgagacag tgtttcgagc
     8101 gggcaccgag aagtaccccc gtgaagccta accccgcatt gagacgttac tctcgccgct
     8161 gaagtcgaga ccgttgaggt tgcgcatggc gagtaccaca tttcatccgc gggcgttccc
     8221 gaccctgcac cgttatttca tgtggtcggt cctcatgaag gatcggtttg aggaggccct
     8281 gtcggagcct gagggccgcc agggtgacgg cttcgcctac ctcgccacta aggccggcac
     8341 cttcatgtct tactggtatg gaggcctata cgtggtcgtc gaggggtgga gggaactcgg
     8401 cctcagtgat ccggtcatag acccactcct tacctcgccc aacgtagagg cgcttcgtct
     8461 gtttcggaac gcgacgtttc actaccagca ccaatggctc caccagaagc accgagcgtt
     8521 catgcagatc gagggcagcg tttcttgggt gcggcagctt cacgcggcct tctcgcagta
     8581 cttccttcgc gaaatgcggg ccaggtacag gtcacatcgg gatacctaac ggcgacgccg
     8641 cggacggtac tgtgaacatc aaggagacga tcgactcgaa ccttgctcgt tccttcgtat
     8701 aaacctcccg cctacgggac cgggattgag taagaaaccg cgggcgtttc tgtagtccct
     8761 acgtgccact ggcaattctc atcgcgcgcc cacatgatcc ggtaaactcc accgtcgtgt
     8821 tgcgaaggct tctcgtcacg ctctgcgccg tggtcctaat gcaggagacg aatctcggct
     8881 ccctgatcgt cggcgcggag tgcttcgaga agtgcgacga cgacattggc cccggtcact
     8941 gctcgccagc ctgcgcgacg tgtgtgtgcg gaacgcacgc gaagccgcat gcgccgggcg
     9001 ccacccggct tccggctccc gcggttacga ccacccgtga gctccctcgt gtggcgcggg
     9061 cgacgggcga cttgcacctg ccggacatcc tgcacgtccc gaagcggttc gtcgcgtaga
     9121 aacggcacag aagaagaggc agtaggggac ggcggcgcag cgcgcctcgt gtttcccgcg
     9181 cccgctcctg tgcccggcgt tctcagcgaa ctctcgggag gaaatggatg tcgattcccg
     9241 gccacctgtg tcggttgttt atggttgctg ttctcggcgg ggcctccgcg ctccgtgccg
     9301 acgaaccgct tcggatcgaa acgtatgtcg agatcgtcct gcaatcgcac cccgctgcgc
     9361 aggtggcccg cgcattcgat gagagcgccg cggcagagtc gcgagtcgcg cggctgttcc
     9421 ccgacccgac gctcgagctg agtctcggcc aggggcgctc cgtgaccgat tcctcggtcc
     9481 gcgccaccga gaccggcatc acgctctcgc aggtgattcc gtggtttccg gcccgcgcaa
     9541 atgccatcga cgccgcggat ttcgctgccg acgcccgccg ggccgagggc tcagcggcgc
     9601 gctggaggct gaggatcgac gcgcgcctcg cgttctacag gctccttcag gcccgggccc
     9661 tggtcgacgt tgcgcgcgcg accgagacgg acgccagatc gctcctcgat ctcgtcaatc
     9721 gcagggcttc actcggtgag acgagagagg tcgaccggat caaggcccgc gtcgagtggc
     9781 taaagcagca acgcgagctg cacaaagctg agcgcgaagc ggaggctgcc gaagccgcac
     9841 tccggacgct ggcggcaacc cccctctccc ggccgctcgt cctaaacggt gagcttccaa
     9901 agccggccga ctcgaccagc acgctcgagg atgcaacccc ggcgcggctc gaccgcagcc
     9961 ccgagatggc ccgcgcgcgc gcggaagccg cccgggccgt ctcccttctc tcgaacgctc
    10021 gccggtcgcg cgttccggac ctcggcctct cccttttcag cctcaaggag ctggaccgcg
    10081 acgcttacgg tgcttctctc ggcgtcgtga ttcccctgtg gaacgcgcgc cgcggcgaca
    10141 tcgcgcgagc cggcgccgag tcgatgctcc gttccgccga cacggaccgc atccgcctgg
    10201 cgctcctcac cgaggtcgag acacggcggc gcgacgtgga tacgtcggcc gccgaggccg
    10261 cgaccctcat gaacgagctg cttccctcgg ccgcggagag cctgcgcctc gcgcggctcc
    10321 tcttcgagga aggggagacc tcgcttctcg accttctcga cgctcagcgg acggcacgcg
    10381 acgcgaggcg cgaggaaatc cgcgcccgct tcgagctggc cgtcgctctc tcggagctgc
    10441 agcggctcct gggccccgaa gaaacctccg ggagatagga acacatgaac acgcgaaccc
    10501 ttcatcaact cacggtcgta acgctttgtc tcgcgctcgc gctcggcggc tgctcaaagc
    10561 gtaagggggc gcctgcgggc gactccgaga agaagaccgg tgaggcgtct caggagcccg
    10621 cggtccccca ccaggtccgc ctgactcccg ccgcgatcgc cgaagcgggc atcaccacgt
    10681 ggaaggtcca ggtcgtggac ctctcgcacc tcctgtcgct gaccggcagc gtgggttacg
    10741 acgagaaccg gctcctgcag gtcgccgcga acgtcaaggg tcgtgtggcg aagatcccgg
    10801 tggacctcgg ggcccgggtg aaggcgggcg atccgctcct cgtgatcgag agcgtcgacc
    10861 tcggtcgcgc gcgcgaggag ttcgtcaagg agttgtcgag cttcaacgtg tcggccaacg
    10921 cctacgagag ggcgaagaag ctcgtcgcgg cgaacgcgat cagctcggga gagtttcaga
    10981 cccgagaggg cgactacctt tccaggaagg ccgccgtcgc gtcggccgaa cgcactctcc
    11041 acctctacgg agacagcgac gatgcggtcg cgcgccttcg ggccggcgcc ggcggccaga
    11101 cgctctcggc ggccgacggc gccacgctga cgctgcgggc acccttcgcc ggccgcgtca
    11161 tcgaccggaa ggtgacgccc ggcgccctct tcgaagcgct gcagcctctc atgaccctcg
    11221 cggatctcgg gtccgtctgg gtgttcctca acgcctacga gaaggacctc gccctcctcc
    11281 acgacggcct ccccgtcacg ctcaggacgg atgcctatcc ccaggagtcc ttccacgggc
    11341 gggtcgactt tctcgggagc gtggtcgacc cgcaaacccg gagtgtccgc gtccgcgcga
    11401 cagtcgagaa ccgcgcggag aagctgcgcc ccggcctgtt cgtcaaagct caagtcgagg
    11461 tgccccgccc gcagagcgag agccatcaaa tcgtggccat cccccaggga gcgctccaga
    11521 cgctggaggg gcgggcgact gtgttcgtcc agacggagcc cggccttttc gtccgccggc
    11581 tcgtcgagac cgggcacacg ttcgagggat tcaccgaggt gctctcgggg gtgaagccgg
    11641 gagacgtcgt cgtgacggaa gggagcttcg tcctgaagtc ggagttcgcg aaggcctcgc
    11701 tcacggagaa ggactgagat gctcaggcgt ctcgtcgact tcagcctcga gaaccggcct
    11761 ctcgtcctcc ttgccgcgat tctggtcatc gtgggtggcg cctatgcgct cacccagctt
    11821 ccgatcgacg ccgtcccgga catcacgaac gtgcaggttc aggttctgac gaaagcgcca
    11881 gccctcggcc cggtggagat ggagcagttc gtgacgtacc cggtggaggc cgcgatgaac
    11941 ggtctcccga agctccggga gatccgctcc atttcccgct acggtctctc ggcggtcacg
    12001 gtcgtcttcg aagatggcgt cgacgtctac ttcgcccgcc agctcgtggc cgagcgcctc
    12061 gtccaggcgc gcgaagcgat tccgccaaac cagggcaacc ccgagatggg tcctgtcacg
    12121 accgggctcg gcgacgtctt ccagttcacg gtcgagggtg acggcgtctc cgcgatggag
    12181 cgccggacga tcctcgactg gacgatcgcg ccccggctgc gcgccgtgcc cggcgtcacc
    12241 gaggtcaaca cctggggtgg ccttccgaag cagtaccagg tcgtcatcga tcccgccaag
    12301 cttcgggcgc acggcctctc cctgaaggaa gtgttcgaag ccgtggggcg agggaacgcg
    12361 aacgcgggcg ggggctacat cgagcacaac cgcgagcagt acatcgtccg cggcgagggc
    12421 ctcgtcggtt cgctcgccga catcgagagg atcgtcctga agtccggtcc cgaaggaacc
    12481 cccgtgaccg tcggagcggt cgcccaggtc cgcgaggggg cgatgctccg catcggcgtt
    12541 gcgaccgccg acggaagggg tgagaccgtg atcggtctcg tccagatgct cgcgggagaa
    12601 aacgccctcg aggtcgcaac gcgcgcccgc aaggccgtcg aggagcttca gccgacgctc
    12661 cccaagggga tccgggtcgt tccgtactac gaccgcgcca ccttcgtccg ccgggtgatc
    12721 cgcacggtcg agacgaacct cctcgaaggg agcatcctcg tcatcgcagt tcttttcgcg
    12781 ttcctcggga acgttcgcgc cggcctcatc gtcgcctcgg cgattccgct ctcgatgctc
    12841 ctcgccttca cgggaatggt cgagtcgcgg atctcggcga acctcatgag cctcggcgcg
    12901 atcgacttcg ggttgatcgt cgacggcgcg gtcgtcctcg tcgagaacgt cgtccggcgc
    12961 ttgtccgagc ccgaaggcca aagcaagacg gtccgtcagc tcaccgccga ggccgctcac
    13021 gaggtcgtcc gaccgatcac cttcggcatc ggaatcatcg cgctcgtcta ccttccgatc
    13081 ctcagcctcg aaggagtcga ggggaagatg ttcaaaccga tggcatggac ggtcgtgttc
    13141 gccctcgccg gctccctgct cctgacgttg actctcacgc ctgtcctcgc gtcgatcttc
    13201 ctgaagaaga caggccacga gcacgagcct cgcttcgtcg ggaagttgcg ggctctgtac
    13261 ctgcgcgggc tcgacatctg cctcgctcgt cgcgcgcccg tcatcctcgg cgcgatcctc
    13321 gccattgcga tcggcgcggt ggtggccgcg aggctcggcg gcgagtttct tccccgcctc
    13381 gacgagggtg acctctcgat cagcgccatt cgcccggctt cggtgggcat ctccgaagtc
    13441 gccgcgagca cgggccggat cgagcgtgtg ctgaagaagt tcccagaggt gatcaccgtg
    13501 gtgagccggt cgggcagccc ggagctggcc accgacgtca tggggatcga gcagggggac
    13561 gttttcgtga tcctgaagcc gaagggcgag tggacgacgg cccggacgaa gggcgagctc
    13621 gtcgagaaga tggagcacgc gctcgacgag gcggtcccgg gcgtcgggtt ctcgttcctc
    13681 cagccgatcg agatgcgctt caacgagctg atcgcgggcg tgaggtccga cgtcggcgtc
    13741 aagctcttcg gtgacgacct cgagacgctg cgccagaaag gcgaggagat cgcgcgcgtc
    13801 gtggccggcg tccgcggcgc tgccgacgtg aaggtggagc agacggcggg cttgcccgtg
    13861 ctgcgcgtca aggtcgaccg cgagcgctgc gcgcgctacg gaatctcggt gggcgacgtc
    13921 cttgacacgg tcgaggcggc gcgggcgggg aagatcgtcg ggacggtctt cgagggccag
    13981 cgccgcttct ccctcgccgt gcgcttcgac gacgagaccg cccgcacgat cgagggcctc
    14041 gggaacgtgc ctgtcgcgac gccgggcggc gctctcgtcc ccctcgggca gctcgcggac
    14101 atttcgctcg agacggggcc ggcccagatc tctcgcgagg cggtcaagag gcgcatcgtc
    14161 gtcgagctga acgtccgcga tcgcgacgtc gcgtccttcg tggcggaggc gaaagcgaag
    14221 ctcgcgagcg gcgtgaagct ccccgacggg tactacatcc gctggggagg gcagttcgag
    14281 aacctcgatc gcgcctcccg gaggctgacg gtcgtcgtgc ctctcgccct tgccctgatc
    14341 ttcgcgatgc tttacttcac cttcggggcg ctgaagccgg cggcgctcat ctacctcaac
    14401 gtcccgctgg ccgccacggg cggcgtcttc gcgctggcgc tccggggact tccgttctcg
    14461 atcgccgcgg ccgtggggtt catcgctctt tttggcgtcg ccgtgctgaa cggcgtcgtc
    14521 ctgatgacgc agatccgcga cctcgaagcg cgatcggaca tgggcgttgc cgacgtcctt
    14581 cgaaaggcct gcgggttgcg cctccgaccc gttctcatga cggccctcgt cgcctctctc
    14641 ggcttcgtgc cgatggcact ctcgaccggt tccggagccg aggtccagcg accgctcgcc
    14701 acggtcgtga tcggcggcct catcacctcg acgatcctga cgctcttcat ccttccgacc
    14761 atttacagcc tcttcggccg gcgaaacgcc gggccgaccc ccgcatgagg tgagccgtgg
    14821 attacgacat catcaatacc ctcgtggcat acatccgtcc gttccagctc gacgcggtcg
    14881 tggacgcgct gcgcaagctg ccgaactttc ccgggctcag cgtcagcgac gttcgcgggt
    14941 ttggccatca cggtgcccat ccgccgcacc gcggcgagac ggcggaggtg gacccgttca
    15001 cgcccatggc gcgcctcgag atcacgtgcc gcggccagga gctgtccggt atcgtggaag
    15061 cgattcgcca ggccgcgcgg accggccacc acggcgacgg aaagatcttc atcaacacga
    15121 ccgcgtgggc ctgtcgaatc cgcaccgacg aagagggccc ggacgcggtc ctcggcggcc
    15181 cctgagcagc tcgatgagtt ttctctccac actcgtccgc gctccggaga tgcgctctgt
    15241 cctcgccggc ggcgcgctcc tcgccgctgg cctggccatc gaccatcttg gcgtcgacca
    15301 cggcgtggcc gtggcgatct acgtcgcatc catgctggta gcgggctggc actggatgcg
    15361 cgaggggtgg cgagaactcg ttcattgcag ggagatcggc atcgaggttc tgatgttcgc
    15421 cgcagcggtg ggcgcggcgg cgctcgggct ctgggacgag gcggctgccc tcgtcttcct
    15481 gtacgggctc gcggaggcga tcgaggagca aagctacgac cgggcccggc gggcagtcga
    15541 gcgacttctc gatcttgtcc cagcgcaggc gactgttgtc cgggatggaa ccgagattct
    15601 cgtcgaggcc tcgcgcctcc agcgggagga tcgaattcgt gtccggcctg gcgaatccat
    15661 acccacggac ggcgtcgtcg aggagggtga ctcggctgtc gacgagtcga gcctgaccgg
    15721 cgagtcgatc ccggtcgaga agaagcccgg agaccgggtc ttcgccggca cgctgaaccg
    15781 gtccggaagc ctggtcttca gcgcgaccgc ggcggcggag gagaactccg tgagccgtct
    15841 cgtgaagctc gtcgaagccg cacagaagtc caagggacgc agccagcagg tcgtcgagcg
    15901 attcacgaac gtctactcgc ccgtcattct tggtgttgcc gttcttctcc tgcttcttcc
    15961 cttcgtgatc ggcgggagct tctccgtgtg ggcacggcac gccgtggtcc tcctcgtcgc
    16021 ggcggctcct tgcgcgctcg cgatgtcgac tcccgtggcg ttcgccgccg gcatgtcggc
    16081 cgcgggtcgc cacggcatcc tgatcaaggg cggccgctac ctcgaggtgc tcggacgcgt
    16141 ccggaccgtc gcgttcgaca agactgggac gttgacgcgc gggcggccgg agcttgtcgt
    16201 cgcggagccc ttcgcgggta cgacggagga ggtgctgctc tcgacggctg cctcgctcga
    16261 gaggttctcg gagcacccgc tcgccgcagc cgtcctccga gcggcagagg taaagggggt
    16321 gacgaacctc cgggctgcca gcggctttcg agcgctcccg ggcgcgggcg tcgagggcgt
    16381 cgtcgacggc gcgcgcgtgc agatccttca gccggacggg ggtggcgaat ccttcggcgg
    16441 tctcggccaa cggctcgagg acctggcccg tgcgggcatc actccggccg tcgtcctagc
    16501 gggcggccgt ccaattggag tcctcggttt tcgcgacgag ccgcgacccg aagcgcctgc
    16561 ggcgctccgc caccttcgag cggctggtat tacgcgcctt gtcgttctct cgggggaccg
    16621 gccgctcgtt gcccgaagcg tcgctcaggc gctcggcgtg gacgacgcgc gaggggggct
    16681 ccagccggag cacaaggttg atgccgtgcg gacgctttcc ttggagtgcg gcccgctcgc
    16741 gatggtgggc gatgggatca acgacgcgcc cgccctcgcg gccgccgatg tcgggatcgc
    16801 catgggggcc ctcgggagcg acgcttcgat cgaggccgcc gacgtggcgc tgatggcgga
    16861 cagcctcgat cacctcgacg aagcattcct catcggtcag cgcgtgcggt cgatcgttcg
    16921 acagaacgtc atcttctcca tcgccgtgct ggcgatcctt atcccggttg ccctctcagg
    16981 gctcatcagc atcacggtca cggtcgtagc ccacgaagct gcggagttga tagcggttgc
    17041 caacgggttg cgggtgctcg gctcgggacg aaaccacgag agggacgaag ggcgtcgcct
    17101 gaacagatga cgggatgacg tcgcggcgca acggacgcgg ttgtccgagt aaaggccgag
    17161 ttcgagcttt cataactgaa tcgtttcatc ggcggcctgt ggcgttaggg ccgtgcacct
    17221 gaaatgtcag attgacgtct aaagcagatg cctcattgag tctgagagtc gggttcagga
    17281 tccttcacgc ggaagagaga aggagaagtc ccatgtccac ggtcaagcga ttcgtccctg
    17341 gcgtcctcct gttcgccctt cttggactgg ctctcccggc gatcgcgcag accggcggta
    17401 tgcagaacgg acccttcgac gggatgtcgg ccggaattgt cgttcccgcc gctgtccggg
    17461 ccgacgggaa gaacggcgcg ttcttccaga cggatctctg gatccgctgt cggaccgtgc
    17521 cggtcgccgc cactctctac ttccacgcgg ccgacgccgc gagcgcaacg ccgaccgtga
    17581 cggtccccgt cacgctcacg cagccggtaa cgtatctgcc cgacattctc tcggcgacgt
    17641 tcggcgtctc gaagggcgcc gggaacatcc gcatcgtcgc ggcagctccg gtttctgcgg
    17701 tccttcgcgt gtacaacaac ggaggaggcg gggcgtttgg cctttcgttc atgggcatgc
    17761 cgtcgacgat gtcgctccag tcgatgccga tgatgggcgg ctctttcggg atggacgact
    17821 tcgcgatgta catgctcggc cttctcccgg aacccggaaa ccgcgtcaac gtgagcgtcg
    17881 tcaatacgaa ttcgacgacc gtcaacggcg tcgtggaagt cctcgactcg gacggattgg
    17941 ctccgaccgg cacgggcgtc acgagccttc cgttctcgat cgccggctac tcgtcgcacc
    18001 agttcaacga cgtgctcctc aacgtccact cgcgcttcgg ttccggagat accggcctgc
    18061 agatccgggt tcgcttgaat cagggttcgg cagggatggt catgtcctac gcggtcgtca
    18121 acgacaacac gacgaacgac ggttatgtcg tgatgggaaa catgatgaac ggcggccgcg
    18181 gcatggggat gatgaactag gggagacacc ggaacgcggg atggcgaaga ggcgaagctc
    18241 ctcactccgc gacggttaac acgagtcggt tcgctcgcgt tcgtccaatc tccagggccc
    18301 ggtagtcccg gaccaccgcg ctctgagcca tccacctggg gatcaaacgg gaaaacggtc
    18361 ggtttctcga acctaaaccg cttgccagat gggggcgctg acgagctcta ggcgccgcgg
    18421 ccctggcaaa cctcggtacc cctccggcga agatgagggt agtcttgtcg agctgacggc
    18481 gacatggagg cggacgatgg gctttctcgg gaagctcgtt tgcggacgca ctttccggga
    18541 acgctccagg gagacggcaa catccgagat gcgtgcggcc ggctcatcgc aattgccgtc
    18601 gccggaccga gcgcggtcca gcgctcccgc cgcctggctg attgttctgc tttcggcaca
    18661 gcttcgcgcc gctgatccct ccccaccggc atactcggcg accgacatca agacgacgat
    18721 tgtcgagaac cttccacccc caatcccgaa cgcttatcac gcgctaggcg actacatctt
    18781 cgcggtccag ccgcttcgag ggaactttca cggggacttc aacggcgacg gccttgaaga
    18841 cattctcgtg gcgcccaact acgcgacctt tcaaccacag ctgcctattc agatatggat
    18901 caatcagggc aacggcacgt tcgttgacca gacgtcgacg gtgattgagg ggccgattac
    18961 tgtcacggga gccgcgacca gcatcttcgt cgccgacttc aacggcgacg gcaggcctga
    19021 tgtgttcttc gtggattcgg gactagagga caaggatccc agcgccggct tcgacggagg
    19081 gatcaatcac gtcctcctca gtcagccgaa tgggaagctc aaggatgtca cgtccgattc
    19141 gttgccccag aatgtgtttc gcttcaatca tgtgtcgagc atcggagacg tcaatggtga
    19201 tggcaccctc gacattgtgc tcaccgtgct gggtgggaga actggtggcg ctgggactct
    19261 gttcctactc aatgacggaa ggggcgtttt cacgtcgacg tccagtggcc tgcccctcga
    19321 catcgcctat tacccatcgg gaccatggtg gcccgccgcg cccgatccac aactccctgg
    19381 atcgaacgga gtgctggacc tcgatggaga cgggcggttg gacctcgtta ccgggaccta
    19441 cagcctcaac gacccggtct caaagaagcg gagcgtccgg ttctttcagc agcggcccga
    19501 ccatagcttt gttgagcggt cgcgaattga gattccgctc gctctcgcga acatcggcta
    19561 ccgcggggga acagtcgccg actccggcgg ggcgggtctg ggtgtggcgg gaatcacggg
    19621 tgcagatctg gacgaggacg gccgcaccga cgtcgtggtg ctctgggagg gcgcctccgc
    19681 gagttacatc cagattctgc gaaatgaagg gaacttcgtg tttacggaca agacacttga
    19741 ctggctcggg acctacgata cgaacgttgt cgctgggggc ctaaagtgga gtgcgtccgc
    19801 ctacgacctg atggacgtga acggtgacgg gcacattgat ctggtgcgtc gcggggcagg
    19861 ctacgacgcc gcgacgctgt ggaatggcgg gttcgcgttt ctcaatgacg gcaccggcca
    19921 tctgaagcca ctggcttacc ggacgcgcag ctccgccgct ggcctagacg acttcattgg
    19981 agcgcttggc tgcagctact gtgcctatac gccgttcctc ttcgacgcga cgggggatgg
    20041 ccagatcgat cttgtgctgc tagacatttc ttccgggaat tcgggccgct ggacgcccga
    20101 gaagcctttc cgagataagg aactctacct tcatgtgttc tctgggcagg gcagtctcgc
    20161 ggggctatcc gcatcctttt cgtatggacc gagctcgccg gctgcgggta cgcccgtagg
    20221 gttcacagac acctcttcgg gggcgacgtc ccgagagtgg aagttcggtg atggcggcac
    20281 gtcgacttcc gcacagccct tgcacacata ctcggccccc ggcctttata cggtgcagat
    20341 gaccgcacgg aacggtacgc aagcgatgac tgcgtcgcgt cgtatcgcag tcacgctcgg
    20401 gagcacgacc cgtcatcaac cagtctcggg gccgccggga ggtcagccgg tcaagaagcg
    20461 gccttagagg ctcgagcgac ccgacggcct cacttccgac ctgttcgccc gccgtcgagc
    20521 gacttccagc acccggtagt cccgaaccac cgcactctga gccatccacc cggggagcca
    20581 ctcttctccc cccgcgaacg tccgtgagtt cgcggttcac acgcaccccg cgagatccct
    20641 cgcggggttt cgtgtctaaa gggcgttcct caatctagag cagcgcggcg agcgtcgtcg
    20701 ccacgaacag aacgccgacg agactccaga cggcgatcca acccatctgc gcgcggcggg
    20761 tcatggcagg cacgcgatgc gggcacaggc ccgcggcgca ggcgcctcga gcaggaccgt
    20821 cccgtcggcc cggacgaccc agaccccgct gcgggtcagg gtgtagacgg gcaagctgag
    20881 gcgcctcgcg agcgccgcat cgaccgaaga cggccgcggg tcggcccggt cgggatgggt
    20941 gtggacgatc gcgagagcgc cgtccggcgg cggcccgtgc caggccgcgc gctcgacggc
    21001 gcccgtgaaa ctccagtcac ggaggacgac gtccccgccg cgctcgcgca cgatccaggc
    21061 tgcgctctcg tacgaggcga ggccgtactc ggcccgtgcg aagagacggc cgagcgccgc
    21121 gcgcagggga gcgttcgaga gccagtcgag catcgtgtcg gggggccgcg ccggaggcgt
    21181 gcacggaggg ccggaggcga tgaggatcgc gaggacgagt gcgggtgcgc ccatgatggg
    21241 ccgaagatgg gctccggccc ggaccccagg gagagccggt ctttggaagg tgggtggtcc
    21301 acttcagcct cccgtggccg cccgcgcagg cgcgaaggag gaggtcgcga cgagtcggcg
    21361 ccgggcgcgc gcgagccggc gcaccgcttc gacgagctcg gtctcatcga gcctgctgaa
    21421 cccgatgctg aggaacgggc gcggttggtc gtcgacggcg taggtctgcg ccgtcctcac
    21481 cgacactcct tcctcgaccg caagcgcgct ccagcgctcg acgtcgaggt cctcggcggc
    21541 ctgcgcccag atccccatgc cacccgtcgg catcgcaaac gagagggcgt cgccgagctc
    21601 cttcttcaaa gccttcgcca gaaggtcgcg ccggttccgg tagagcctcc tcacgcgccg
    21661 cgcgtggcgc gcgacgtcgc cctccgcgag caggcgggcc acggccactt ctccggccag
    21721 gtcgccctga atgtccgaga cctgtctgag aatcgcgaga cggtcgacga aaggccgcgg
    21781 cgccacgacg tatccgatcc tcagcccggg agccaggatc ttcgagagag tgcccacgta
    21841 gacgacgaca cccgccgtat ccacactggc catcggaagg accgggcgtg actcgtagtg
    21901 gaactcgtgg tcgtagtcgc cctcgaggat cgcgatccga tgccgccgcg cgagctcgag
    21961 aagctgaagc cgccggccgg gcacgagcgt gacggtcgtc ggacactggt ggtgcggcgt
    22021 gaggtacacc gcccggacgg cgggctcgcg gtcgacgagc gcccgcacgg cctcgacgtc
    22081 gatcccatcc gcgtcgaccg gaatcggcac gacggaagct ccggcctggc gcagcgcttc
    22141 gcgaaccggc ttgcttccga gctcctcgac ggcgacgacg tcgccgggcc cgagaagggc
    22201 gcgggccgcg aggtggagcc ccatctggct cccccgcgtg acgatcacgt tctcccaacc
    22261 cacggccagg ccgcgcgtcg cggcgagcat ctgggcgaga gcctgtctga gatcccggct
    22321 cccgtacgct ccgttttcca gctcgagggc gctgcgtccg cgcgtctggt agtcgaggac
    22381 gcggtggccg tccatgcgga gcgccgttcg gtaggcgcgc gccagcgcct cgacagggac
    22441 caggcgcacg tccgcgctcc cgttgccgaa gtcgaggttg cccggccgaa agagcggccc
    22501 gggggaaacg ctcggagacg ggacgagttc gaagcccacc cgggacggga ccttgcgccg
    22561 cagaacgccg aacgagcacg gaacctcgca cggacaggtc ccggaaacga aggtcccgct
    22621 cgcctcggag ccggtcgtcc agccttctgc ctccagctcg cggtaggcgg cgacgacggt
    22681 gttgcggttg agcccgagcg cgtgcgcgag cgcccgcgag ccgggcaggc gatcaccggg
    22741 gagaagccgg ccgcgtcgga tgtcctgggc gagtgaacgg acgatctgaa ggtagaccgg
    22801 cagctcgctg gctcgatcga cggggatcgg aaactcccag cgcgccatag agggccaagc
    22861 ctcctcctcg cttccgggat cgtagggaag ggcgtagcat taccctaatc gaaagaaaga
    22921 aagctttgaa tgttccaggt gcatgaaaga ccggccgggt ccggcggagg ggactttgga
    22981 gctcgaccag attcgcgcat tcgtgttcgc cgtcgaggag ggctcggtgc agggcgccgc
    23041 gcggcggctc caccggtccc agccggcggt gtcccgcctg atccagtcgc tggagcgcgc
    23101 cctcggagag agcgtgctgg accggagcac gaggcccgcc tcgccgacgg agttcggccg
    23161 ggaggtgctc gacgaagccc gtcgggcgct cgacgcggtg gatcgcctcg agggccgcgg
    23221 ccggacggac gccggcgcga agcgaaccac gatccggctc ggcgtgtccc atgcctacac
    23281 gcggatcgtg gcgtcccctc tcgtgtccgt ggctctgagg gcctccggga atcccgctct
    23341 gcgcatccac acgggttgga gcattccgct cctcaaggag ctgctggagt cccgtctcga
    23401 cgcggcgctg atgggattcc cgggacgctg gtctccgccc gccgacctcc ggagcgagcc
    23461 ggtccgcgag gaggagctcg tcgtcatcgc gccccgggac acgaagatcc ggggcatcac
    23521 gcggctccgg gacctggctc cgcatccctg ggtcctgaac tcggagggct gcggactcag
    23581 agcgctcctt cagagggagt tcgccgagtc cggccggagc ctggacctcc acctcgagct
    23641 gcagggaagc ctctcccagc acatcgagtt cgtggccgcg gggttcggtt tcggactggt
    23701 gccgctctgc gccctgacca ttcatcctca gcgacgcgag gtccgaagag tcccgctgaa
    23761 ggagacgctc gcgacgaacc tgtggatact gtggcgcccg tctgaccgcg ccgcggatgc
    23821 gcgctgggcc gggatccgtg ccgcgctggt ccgctccgcc acgccccggg cgctctgagc
    23881 cgagaacgag ttgaattcag tggccgaagc gtcgctgcag ctcggcatac gggatggcgc
    23941 cgtgggcgaa gccgaaggtg ccgtgatcgg ccatctcccg cgcggcctgc tcgatccggg
    24001 tgagggcggc gcgggcgagg gtgccgccga cgctgatcct ggcgacgccg agcgaacgca
    24061 gggtggccgc gtccagaacc ggctcgacca acccggccac gacgttcacc ggcgcgccga
    24121 tcgcggcggt cagcctgccg atctcctctg acgcgctgac gccgggaacg aagatgcagt
    24181 cggctccggc cgcgaggtag cgctcggccc gccgcacggt ctcggcgaag gcgtccgggc
    24241 gcctgagcat gtaggcgtcg gtgcgcgcgt tcagcacgaa cgaaccggac ggcgcggcgc
    24301 tccgcgccgc ctcgagacgt tcgcaggctg cctccaccgg gagcagttgt ccgggctgga
    24361 tcacgtcctc cagattgccg ccgaccaccc cgagctcgac cgcagcggcg accgtcgcgg
    24421 caacgtgacc gggagtcgcg ccgtagccgg attcgaggtc ggcgctcacc gggcacgcga
    24481 cagccgagac gatccgcgcg atgcagtcga gcatctcggc cctctccatg gtgccgtcgg
    24541 cggcaccacg gctgaaggca atgccggcgc tggtcgtcgc gatcgcggcg aacccggcct
    24601 gttccaggat ccgtgccgag cccccgtccc aggcattcgg gaggatgaag ccggcgccgg
    24661 ggcgatgcat cgccaggaac gccgtggcgc ggttcggggt tgcggcctct gtcatgcgtc
    24721 cgattctatt catggacctc gtgtcgaaag cgttcgacga gctcctccgg gccattcaga
    24781 cggtacgcga tcgggagcgt cagtatgcgg tcgtcggtca cccgggccgc ccgcgacggg
    24841 ccgagggtag actgcccgcg ctcggatcgg atcgcgcgtc gccgacgagt gggagggggc
    24901 ggatgactct cgagcccggc accaggctcg gtccctacga ggtgtcctcg cctctcggcg
    24961 cgggagggat gggcgaggtg taccgcgccc gtgaccctcg cctcgcgaga gacgtcgcga
    25021 tcaaggtcct gcccgaggaa ttcttcgagg gcgaggaaag aagagtgcgc ttcgaaaggg
    25081 aagcccgcac gcttgcgagc ctgaaccatc ccgggatcgc cgcgatctat tccttcgaag
    25141 aaatcccctc ttcgtcttcg tcttcctccg cgacgcgcca tatcctcgtc atggagctcg
    25201 tcgacggcga ggacctcgcg cagcgcctcg gacgcggcgc catcccggtg gacgaggcgc
    25261 tcgcgatcgc caggcagatc gccgaggggc tcgaggcggc gcacgaaaag gggatcgtcc
    25321 accgcgacct gaagcccgca aacgtgaagc tcacgcgcga cggggtcgtg aagatcctcg
    25381 acttcggact cgcgaaagcg ttcgagggag acgcagcctc ctcctcgagc tcgccgtcgg
    25441 tgacgcaatc gccgacgatg tcgcgtcccc tgaccgaggc ggggatgatc ctcggcaccg
    25501 cggcgtacat gagccccgag caggcgcgcg gcagaccggt cgacaagagg accgacatct
    25561 ggtccttcgg agtcgtcctc cacgagatgc tcacgggaaa gcgcctcttc gccggggaga
    25621 cggtcagcga cacgctcgcc gcggtcctcc ggcaggacct cgatctcgcc gcgctcccgg
    25681 cggagacgcc cgaccgcgtc cgccgcctcc tggcccgctg cctcgtgcgc gacccgaaac
    25741 tgcggctccg cgacatcggc gacgcgcggc tcgagctcga cgcgggcggc gccgatcggc
    25801 cggcggacgt
//
LOCUS       DAOMFY010000025        25672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_622_length_25672_cov_7.914822, whole genome shotgun sequence.
ACCESSION   DAOMFY010000025 DAOMFY010000000
VERSION     DAOMFY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25672
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_25672_cov_7.914822"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLB01_03915"
     CDS             <1..788
                     /locus_tag="PLB01_03915"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HQQ76477.1"
                     /translation="ALPDLARAADAIAARLDAGGRWFYIGAGTSGRLGALDAAELPPT
                     FGTDPALVVALLAGGKRAMFEAVEGAEDNADAGAVDLLRAGLTARDAVVGIAASGATP
                     YVRGALREAKAKGALTVSVVCRPGAPLLGEAEIGILLDTGKEVLAESSRLKAGTAQKI
                     ALNMLSTAVMAKRGLVYEGEMVAMQPTNEKLKRRAIRIVRDIAATSEEKAEDLLRMVN
                     WHLPSALIAAKWKLDASAAVLHLSKKNSNVAAALSQPPEEGRG"
     gene            785..1723
                     /locus_tag="PLB01_03920"
     CDS             785..1723
                     /locus_tag="PLB01_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HQQ76478.1"
                     /translation="MSSGAKSSSSAPVFVDLHIHGAFGVDVLTASAIDMDRLAAGLWA
                     RGVSGFLPTLVPLPLPEMAAAVTRLSAWMRTRADGDGMGALPLGLHFEGPFVSHARCG
                     ALHRKDFLDGTDGLALGAFFEIASSAPGRGVTTLAPEIPGGIAVVKEFVRRGFVVSIG
                     HTEADVAALEAARAAGARHMTHFGNAMRPLHHREAGPIGWGLVTDGVSVDVIADGHHL
                     SREMLKLVFRAKGDGRVALISDAMPAAGLGDGSYSVWGETLEVEGGAARNAAGALAGS
                     TALLDDCVRRLAETGLPEEVAVRAASDVPRRILASG"
     gene            1766..2101
                     /locus_tag="PLB01_03925"
     CDS             1766..2101
                     /locus_tag="PLB01_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQQ76479.1"
                     /translation="MDLEIRDDGRVTILTLKGDLGIGDSETLFKRTVSRLLEEGKVHL
                     LVDCTGLRFVDSTGLGALVRALTTSQHEGGQTKLLGVGPHMRKLLEMTKLDSVFETFT
                     DLEKAVSSF"
     gene            2108..4522
                     /locus_tag="PLB01_03930"
     CDS             2108..4522
                     /locus_tag="PLB01_03930"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQQ76480.1"
                     /translation="MSSGWESPGPGGAADASALAELAACEGFAQVAAFVARRAAAAAG
                     ADAALLFAPDGAQAGFVCAGAWGEGTPKALRRAAPRDRGLLHELLRDRSAVLLGRDRF
                     TRSDDALLTTAPAGAEACLLLPVVQDRAVAAVVAFFFRRAPEPAPVLARTLPFLEAVV
                     PALAKARAAERKASGMLQAIERLTSLFDLSKAFGSTIDLAELDALITRKAADFCTCEV
                     ASLWFLQDDEVVLAATAVNPNYDVASPPASVGASVVANVLADTEVLRRNSLPEDDPIR
                     TADPAYPVHALLAVRLVEDDAPVGVLALANKRGRHPEFSDGDEELLVDLARQAVRALH
                     NARQYEAEKKVEELDALLTVSREITSTLDLDKVMNAIVNSTAALIRYDRCAIGIQDRG
                     RLRLGAVSGILELDRSRPDLKRTTEILEWVYGGGADVSIVESEDGTLETSRPETTEKF
                     RAFFAEGKLKSFYGILLKDDEGPLGVLGFESSERLEFDEETRSLLQILVNQATVAVRN
                     AQLYRQVPLAGFLQPFLEKKKKLLGVPRSRRVTWSLGAIGLLVVLFLVPWRFRLTGNA
                     RVLPARRAAVTAGVDGVVARVLKLEGDSVKAGEVVAVLENEAYAASAAAARAAYDIAE
                     ADVSRFRAAGDAAAAFEAESHRKELAARIALEDERLARTRLVSPVEGVVVTPRVQERV
                     GQNLARGAELCVVADTRTMVAEVAIPEEEAARLRPGQPAEVKLNTYPGRTFAGSVTRV
                     GAVVREEGKDRFVVAEIGIENVDGLLKTGMQGRGKVRVGWSSIATLVLRRPARWLYAK
                     LWPVLP"
     gene            4519..6705
                     /locus_tag="PLB01_03935"
     CDS             4519..6705
                     /locus_tag="PLB01_03935"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQQ76481.1"
                     /translation="MSAAPASRPGLAAERGAALLAEKFGARGERPSLKKNLVVRPQVQ
                     MGETRIMVKDPDRTKYYVFADYEWRLIEFFDGTRTRPEIVADYGVRYPFDPIDLTLVL
                     EYEEMLRKIDFIERSGAEKSLQLLAHSKEARQRAAEEKAEGFNPFFLLFHVLDPEEFL
                     KRTVKYVRWIWTPPVVIAWSIAVAWTIGVFVLHWEPIFTGTYELYAFLHKPFIDAVHF
                     FFILSFLGFFHEYGHAYAVKIYGGEVHDIGLALLYFTPAFYCDTTDALLFENKWHRMW
                     VNTAGIYVEGFVCAGATALWVASYPDTLLHELAYKTMLFTGISTIFFNVNPLVKIDGY
                     HALTSFLEMPDLREESFHYLGLVFQKHVLRLDVQVPVTTTRRRRIYWIYGPLALAYVG
                     VIMSFIGGVLNNLYHKIVPDFAVVLLVASLAFIFKKRVRLVFRTARLLYLDKKEFLMS
                     RAARPRLAALAAVLLLLLFVPFSRETLTSEGVLAPSARLRVEAPEDGRVEAVLAKESD
                     AVGAGDVLVRMSSAAATAAAAESAGDRERSAGAARASRSEGDAGSAYRDERKSESADV
                     ALTGAQARLERLAVRSPLAGLVLTPRTEDLEHRFVSKGTMLLEVGDCRELVAPLPVSE
                     RLHDDLRVGADVTAYVRQRPLEAIHGTVRRIAPATAGQPGTAAPGVESLPGEMPDRFV
                     ALAFFPNPGGTLRPGSPVRAKIAGPRRSWAARGFRILGRWMRTVFW"
     gene            complement(6776..7165)
                     /locus_tag="PLB01_03940"
     CDS             complement(6776..7165)
                     /locus_tag="PLB01_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76482.1"
                     /translation="MANEVRIKLTPEQKAKIKSATGQVMSEIRVGSLGNSPAVTSAKT
                     LSARSARETMSARSARETLSARNARETLSARNARETLSARNARETLSARNARETLSAR
                     SARETLSARSAREALSTRATRATDNDE"
     gene            complement(7351..8712)
                     /locus_tag="PLB01_03945"
     CDS             complement(7351..8712)
                     /locus_tag="PLB01_03945"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ76483.1"
                     /translation="MTGKEGRLAIVEDDRLLLDQLVFALKGTFEIATAPDAMAGLALA
                     DEPFDLFLIDLQLPPSSEADEGLRLLTALRKRRPDAAIVVMTGEAERKWALRAVELGA
                     YDFFQKPVDKAELLLVLRRALERSRILAENEALKNEVVRHRSFPRIIGTSPPMRALFS
                     KIEKVADTDVTVLLQGESGTGKELVAEAIHRGSPRSGGPFVAVNGSALPEGLAESELF
                     GHEKGAFTGAVATRPGRFELAHGGTLFLDEVSTLSPAVQAKLLRALESRSFEHVGGKR
                     TIHADIRLVAASNEDLSLLVKKGAFREDLFYRLNTVLLVLPPLRERREDIPLLVEFFS
                     RRAARRHGKAQKGFTPEALEALKRMPFPGNVRELEHLVEMLTLMVEEEAVTPAHLPAA
                     NLSAPPPGTETGEPLAAAVARFEKELVARAIARSGGVKARAAEALGLDPNQMKYLCRK
                     YGL"
     gene            complement(8709..9368)
                     /locus_tag="PLB01_03950"
     CDS             complement(8709..9368)
                     /locus_tag="PLB01_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ76484.1"
                     /translation="MDSVLLHDMRNMGFRLGLVLENLEEHFGDPDFKRSVVELLQGTR
                     DKLDTMVDRWTAHMDSILIKVEVDVNDLVREVLRLAHVRGSGGPGPLGVTVELAPLPR
                     VWGDPHYLRDALLSVVQNALEAVAPAGGRVTVRTREERRGRKRWVVVEIEDDGPGMSE
                     EFVQTRLFRPFQTTKPEGVGLGLYTSSQILLFHRGDLVVKSREGQGTLVRALLPVEAG
                     S"
     gene            complement(9424..10218)
                     /locus_tag="PLB01_03955"
     CDS             complement(9424..10218)
                     /locus_tag="PLB01_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ76485.1"
                     /translation="MRRDSLRGFWTNANGLVIALLLAAPLSAETPGSAPAPKGPLTAT
                     SNLMVEQEVSVTTRVSGVVDSIAADRGDLVKKGQALAVLDQREFVLDRQAAEETLRVS
                     RADNDRFEELFRQGLASRAELEQKRSRFELAAVELERAKLVIDRSVVRAPFDGVVADR
                     FVRAGEKVLLDEHKPLFKVMALEPLLARAYLPTAALGSVRVNDEVVVSSTEFPDVRTT
                     GRVSFVSPVVDPASGTIQVLVRVPRDAKKVLRPGLGVKLVFAPSAR"
     gene            complement(10236..11321)
                     /locus_tag="PLB01_03960"
     CDS             complement(10236..11321)
                     /locus_tag="PLB01_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HQQ76486.1"
                     /translation="MFRGLTVVGMVFVNNPGDWGHLYRPFDHAEWHGWTPTDLIFPWF
                     LFIVGVAGVFSLEKRMAQGADRGDLARHAFRRGMTIVLVGWLMALYPFVPFFERLARL
                     RIPGVLPRIGITFVLGTWILLAVWKRRSAGVAAAVTLLLALHTWILLGTGYDLTPAGN
                     VQRAVDLAVLKGHLWKNAQGWDPEGLVSTLTAIATLLTGTLTGLLLRSEATVKAKVAR
                     LVAWGTAATAAGLLWGKVLPINKNLWTASYVVFTSGAALLAIGLCVIVIDVLGGKKPF
                     APFFTFGTNPLLVFVLSGLLGKTMGLIRVSDAAGKTISLHTVLYRAGFSWIADPTLRS
                     HAFALATVLFWWAVLRVFEKKGWFWKI"
     gene            11393..13039
                     /locus_tag="PLB01_03965"
     CDS             11393..13039
                     /locus_tag="PLB01_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQQ76487.1"
                     /translation="MKIFLESEYARPQRLSSLLFLFLSSILLSLSSCTTIPASLHVTP
                     ADAPLARRVAKLAAGSGAHMGIVALHVESGRRFELNAAEPFEAASVIKIALLAEAAAR
                     DAEGTLDLTDRWKLTAKSLAAGSGMLDEFDPGLTPTNRDLLSLMIAISDNTSANRFID
                     LFGADAVNARMAKLGLADIRLVGRIPDLGKGEGKWAPLGVMTPNATAEYYRRVATRSL
                     LDPASDRVIAKLLAVQHTRDRLPRLLLDETSSKWAGKTGSYGGVRNDSGILTTKKGRF
                     VLVAFADRIPDEKGATAQATRAMGEIAAAIVNTWSETLPDVAPALEPPAVPALRPAVP
                     RMETTLAEARAATQPSSLVRVWRDTDRRFWDAWALAGGETRDACLIPMPNTWWEGFRP
                     SKIEPISALILHHTSQATDEECIALFQRPESRVSSHFLVGRDGRLYQFVSLEHRAWHA
                     GPSTLHGRLALNRTSVGVEITGDGNRYPFTPAQIETVVRLVGVLTAMFDIPAPWIAGH
                     QHIAPDRKDDPGVLFPWNDVVRRGLELSESLRKKGLPKGD"
     gene            13080..13964
                     /locus_tag="PLB01_03970"
     CDS             13080..13964
                     /locus_tag="PLB01_03970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HQQ76488.1"
                     /translation="MHGRLSRLDDAETLRGFVRDLREGIYVTDRDGRVLDANGAFLAM
                     FGVSSLDELRASRAADLVVDVARREAELALLHRDGAVRDFELEIRRAGGEVRTVIDSA
                     TCVTDPETGEVFYHGILIDITDRKLLEQRLLDQSLRDPLTGCFNRRYLNTFERRHASG
                     SHSSFSGAWGCIMVDLDYFKQYNDEFGHEAGDAVLNRVARFLMRQTRAEESAVRMGGD
                     EFLLLLDGADAAATEFAARRLQSTADRELLVPFSMGWAAREDGETLEQTIARADQALL
                     KSRGRTRSGERPRLVLPA"
     gene            complement(13961..15100)
                     /locus_tag="PLB01_03975"
     CDS             complement(13961..15100)
                     /locus_tag="PLB01_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HQQ76489.1"
                     /translation="MTQPISRRGLILSSAAAAASSALPEALFAEAPSRPSSAPVSLSY
                     RPLVLKLAHTWTIARGSSDEKKNGLLTITAEGVTGYGEAAANKRWNQSFETSEAAYAK
                     VEKAVQGLSPWDHLTWLEKAEAAAGNDHEVVAALDIALWDWKGKRLGRPVHQLLGVPS
                     SGMPVTTFSIGIDTAEVMKQKTKEAAAFPKLKIKIGLPSDEENIAAIRSVSSQALTVD
                     ANEGWATVDEARRKILWLNGVKPLYLEQPMPAALDGEMVKLRGLATYPVLGDEGVLTA
                     ADVAKKAGLFDGVVVKLAKAGGITRSWEQVVVARAVGLKTMCGCMIESSVGIAAGLAL
                     GPLFDWLDLDGNLLLAKDPFHGLRIDKGVWAMPEGPGLGVTPVQA"
     gene            15099..16976
                     /locus_tag="PLB01_03980"
     CDS             15099..16976
                     /locus_tag="PLB01_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQQ76490.1"
                     /translation="MRRLWWNPGMRFEGRPACRTAAARALAAALLLFSFEEPSLSASP
                     DATPAPAKTAVVVAAVQDLYVRPDETSSVDDQAILGDRVTVLEDAAGFAKVKTAAGEI
                     AWIPERALRRGAGADAPAHKVARVTSNFAHVYPWPSFTKQKPLLTAPVGATMAFSDFL
                     EGKEGGASDAYSWVKVSLPDGREGYVSARDVEWGAADTPPSKKSSSSSPSSWIAFGKR
                     FLGAPYTWGGTTPLGFDCSGLMTRIFSEHGVTLRRNSYEQAFQDPQLVPVAFERLRPG
                     DLLFFGTDDKIDHEAMWIGDGTVLQSTRHNVPGVQVTSFDSPYLKPLFRYARRLKKIF
                     LEGEEGPRLTQAKVRDLQRALDSIADGSGARFGIYFKDLTTGQTLSKNSSVSFHAAST
                     MKTPVLLELLRRVDNGTVRLSDELPVKNEFKSLVDGSPFSIGLEDNDGPTVAYAGKTA
                     TLEFLAREMIVRSSNLATNLLLTLLGPAEVQKFTDALGAPTVHVRRCVEDTKAFEKGM
                     NNETDAAGMGTVMEAVVRTPKLSAAAKAKAWEILVGQTFNEEIPAGLHPQSGAVVAHK
                     TGSISSVQHDAAVVRLPDGREYVLVLLANDFGANEKGRERVNAAARKMSRAVWEAMIA
                     P"
     gene            16973..17860
                     /locus_tag="PLB01_03985"
     CDS             16973..17860
                     /locus_tag="PLB01_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQQ76491.1"
                     /translation="MKRGVVPALLVIYVVWGSTYLAIRWGLETIPPFTLSCSRYLVAG
                     TALLLFVKARGAPRVALRDLGPAFLTGGLMLLCGNGGVVWAEQRIASGLAALLVAVEP
                     LFIVLLQATLPQERRRPSGRALVGVALGIAGVILLVGPVGLGGARVDLAGAAAVLFAA
                     FAWALGSLLSRHLKPSVSPLQATALQMLAGGVLLGGASGLAGEWARFSPGLVSAKSVA
                     AVAYLVVFGSLVAFTAYVWLLRAASPALVSTYAFVNPVVAVFLGWLLAAEPVSARTLV
                     AAAVIVGAVVLITLGESRR"
     gene            17917..18390
                     /locus_tag="PLB01_03990"
     CDS             17917..18390
                     /locus_tag="PLB01_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HQQ76492.1"
                     /translation="MKKTFVVFALACVALSAGAVLAADAAPKHGLATVDSAWVKAMLA
                     NDAAACAALYADDAVLVLPGSGAIKGKKAIADAYAGWLSQVKVTEAAVMDNHYRSSGK
                     VSAGWGAFKVTTVPKAGGAPTTEVGTWCAVAAQQKDGSWKYVADHASDDPAPAKK"
     gene            complement(18394..18966)
                     /locus_tag="PLB01_03995"
     CDS             complement(18394..18966)
                     /locus_tag="PLB01_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76493.1"
                     /translation="MTPSRRRSVLLAAGAFVAAAMGYAYWLSLTPATHPDHDHGILNL
                     DAGGRLVVETRDGKSRNLVGKPGHALVLHFFSTTAPGASDELAGIFLQQEALKGDRDV
                     EFVLIAHDKDFATLDAWLKEKNLVPPFPQTLVLDPSGDTTTKLNSKRPNETMFFAADG
                     KLASQARGSLTWPDGAAPHLAKARSGESIE"
     gene            complement(18963..19847)
                     /locus_tag="PLB01_04000"
     CDS             complement(18963..19847)
                     /locus_tag="PLB01_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76494.1"
                     /translation="MRLIRRLTAAGFALAMAREPAGATDVDPVELIRRVVKSQRRVET
                     AFEGATFDQREVQITWGAKGRPEKVESRLFYVFAGENGAESSRELVEVDGRPATEDEK
                     RKAAEKDEKGRRRRVEEKAEAAAKAPPRVAGDEDDPLVGSRRLSELIGRYTIRLVGEE
                     VIEGRPAYVLDFSPSPTAPSAKSLGDRALNALEGRAVIDASTFQVRSVEARLTRPLKV
                     AGGFAANVKEADVSYTGQPAGGGLWFPCAVDFRVTGKTALFFRLDTSFRATFTNLRRF
                     NVETETDVRPEADAGSRP"
     gene            19912..20817
                     /locus_tag="PLB01_04005"
     CDS             19912..20817
                     /locus_tag="PLB01_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HQQ76495.1"
                     /translation="MRVIVTGGTGFIGPKLCERLVAKGHDVIALTRDASRSRDHVHPK
                     VRVVSWAEGAAWEGFVDGAGALVNLAGETIAQRWTASAKERIAKSRLNAAARLRAAAE
                     KAAQKPGVLVNASAVGYYGPHGDEILDETAPAGNDFLARLCVDWEAAARAFEPLGTRV
                     AMLRTGVVLGAGGGALAKMLPPFKAFAGGPLGSGRQWMSWIHEADLVEMYVWAAENPA
                     VAGPVNATAPKPVTMRDFATALGKALHRPSFMPAPAAAIRLALGEMATVVLDGQRVVP
                     KKALDLGFAFRFPEVLPALQDVVGD"
     gene            20817..23075
                     /locus_tag="PLB01_04010"
     CDS             20817..23075
                     /locus_tag="PLB01_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQQ76496.1"
                     /translation="MALVGRSVPRPDGPAKVTGRARYVDDVAVPGTLVGATLRSTVAR
                     GRLRGIVRDPSFDWAGLTVVTAENVPANVVALIEEDQPVLAADDIRHAGEPLALVAGE
                     DALRVQRALQHLRADVEPLTPVLTVKDALARGAQIYGSDNVFRTVHIAKGDAAAAIAA
                     CPVQLSGTYTTPWQEHIYLEPQGVLAFWDENGGAHVLSSCQCPYYIHKGIKRTFGLTD
                     ERVDVTQAVTGGGFGGKEEYPTILALHALLLAKASGRPVKMIYDRKEDIEATTKRHPS
                     SVSITSGCDRDGTLRAVTIRIVFDGGAYVTLSPVVLSRGALHAAGAYRWEHVAIEAQA
                     VATNTPPNGAFRGFGAPQTIWAMERHMDRIARTLRLDPLLLREKNLLRVGDTTPTGQF
                     LKESVGVARCVEEAKKASRWEERRASPAPAASGMFRLPPPGAVTARKARGIGASVYLH
                     GAGFTGSGELAHRARIAVDLLADPKTGRARFRVRGGSTDIGQGTETVFRQIAAEALWV
                     SLDRVEFATPSTAAVPDSGPTVASRTVMVVGGVLEAAARSLFAQIEGERQQKGGSFDD
                     AARRLLADRGTVTVEREYEPPPGLAWDDATYRGDAYPVYGWGCAIAEVEVDLDTFETS
                     VLGLWSAHDVGKAIHPTNCKGQIEGGLLQAIGWALQENLVLREGRVVNARLTDYVIPT
                     ILDAPRFTTLLVEDPFSRGPGGGAKGLGEIPMDGGAPAVAAAVEHATGIVCDDLPLLP
                     ETLFALSKEARK"
     gene            23078..23548
                     /locus_tag="PLB01_04015"
     CDS             23078..23548
                     /locus_tag="PLB01_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQQ76497.1"
                     /translation="MRFTLNARPATVDAPPAARLLDVLRGVLGLTGTKEGCGEGECGS
                     CTVLLDGAPVNACLVLFGQVEGRAVTTVEGLSDPASLSPLQEAFVKEGGAQCGICTPG
                     FLMSAEALLAAIPQPGETDIREALAGNLCRCTGYQRIVEAVRAAAKEKTSREPG"
     gene            23535..24437
                     /locus_tag="PLB01_04020"
     CDS             23535..24437
                     /locus_tag="PLB01_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQQ76498.1"
                     /translation="MSLAELPLHAFERPASLADACATLSRRAGKGEETVLVAGGTDWV
                     VAQRLAPLSLGGELPLVLDISRVPELLGVRIAGERAAIGAAATYFELRHHPVLGRRVP
                     MLTAMARDVGALQIQARGTLGGNLATASPAADGSAALMALDADVVVASVHGERRVPIG
                     QWFTGYRRTARAADEIIARVEFTLPKTGAHQLWRKVGTRAAQSISKVALAAVGEYERG
                     RCVRVGLGMGSVAPVTVALPAARALAVSPGPLAVPDDVLDAAVRRDISPIDDVRSTAA
                     YRAHVASALVRGFFRTLRPVSNAG"
     gene            complement(24448..>25672)
                     /locus_tag="PLB01_04025"
     CDS             complement(24448..>25672)
                     /locus_tag="PLB01_04025"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQQ76499.1"
                     /translation="FQDLQFRVFLEALDVLRGAIIVVMALDHVRDYVTNIPFDPVDMS
                     QTFPALFFTRWFTHFCAPGFSFFAGTAAFLSLSRGKTPESLARFLASRGLFLIALEFF
                     VLRFVWLFNLWPVAFIATLWSLGVAMLVLAFLLKLRLGPRAIAGVGAVLVAGHNALDF
                     VDKLPLTGLAGAAWKILHAGGMIPFGTPPAGFPLPGLFNLEVGYPAIPWPGVMALGYG
                     FGALLGRPRDERRRLTFALGFALTTAFVVLRLANAYGDPRAWAPQKSAVMTFAAFLNC
                     EKYPPSLCFLLMTLGPLLLTLAVLDRAPGPAGRFLAAYGRVPMFFYVAHLPIAHTLAV
                     AVAAAQSGGLSGPLVHWAAFQSPAFIVPPPQGFGVGLPAVYALWIAVVLALYVPCVRY
                     GDFKSRSRAAWTSYI"
BASE COUNT         3798 a         9143 c         8879 g         3852 t
ORIGIN      
        1 cggcgcttcc ggacctcgcc cgcgccgccg acgcgatcgc ggcgcggctc gacgccggcg
       61 gccggtggtt ctacatcggc gccggaacca gcggtcggct cggcgccctg gatgccgccg
      121 agctcccgcc gacgttcggg acggaccccg cgctcgtcgt cgcgctcctc gcgggcggga
      181 aacgcgcgat gttcgaggcc gtcgagggcg ccgaggacaa cgccgacgcc ggcgccgtcg
      241 acctcctccg cgccgggctg acggcgaggg acgccgtcgt cggaatcgcc gcgagcggcg
      301 cgacgccgta cgtgcgcggc gcgctcaggg aagcgaaggc gaagggcgcg ctgaccgtct
      361 ccgtcgtctg ccggccggga gcgccgctcc tcggcgaggc ggagatcggc atcctcctcg
      421 acacggggaa agaggtcctc gccgagtcga gccggctgaa ggcgggaacg gcgcagaaga
      481 tcgcgctcaa catgctctcg acggccgtga tggcgaaacg cggcctcgtc tacgagggcg
      541 agatggtcgc gatgcagccg acgaacgaaa aactgaagag gcgcgccatc cgcatcgtgc
      601 gcgacatagc cgcaacgtcc gaagagaaag ccgaagatct tcttagaatg gttaattggc
      661 atttgccttc cgcactcatc gcggcgaaat ggaaactcga cgcttcggcg gccgttcttc
      721 acctttctaa gaagaattcg aacgtggccg ctgcgttgag tcagcccccc gaagaaggcc
      781 gcggatgagc tcgggagcaa agagttcttc ttcggctccc gttttcgtcg acctccacat
      841 ccacggtgcg ttcggcgtgg acgtgctcac cgcgtcggcc atcgacatgg accgtctcgc
      901 cgcggggctc tgggcccgcg gcgtctccgg cttcctgccc acgctcgtcc cgctgccgct
      961 gccggagatg gccgcggccg tgacgcgcct ctcggcgtgg atgcggacgc gcgccgacgg
     1021 cgacggcatg ggagcgctcc cgctcggcct gcacttcgag ggcccgttcg tgtctcacgc
     1081 gcggtgcggc gcgctgcacc ggaaggactt tctcgacggg accgacggcc tcgccctcgg
     1141 cgccttcttc gagatcgcct cctccgcccc ggggcgcggt gtgacgacgc tcgcgcccga
     1201 gattcccggc gggatcgcgg tcgtgaagga gttcgtccgg cgcgggttcg ttgtctcgat
     1261 cggccacacg gaggccgacg tcgcggcgct cgaggcggcc cgtgccgccg gcgcgcggca
     1321 catgacccac ttcggcaacg ccatgaggcc gctgcaccac cgcgaggcgg ggccgatcgg
     1381 atggggcctc gtgacggacg gcgtgagcgt ggacgtcatc gccgacggcc accatctctc
     1441 ccgggagatg ctgaagctcg tcttccgcgc caagggcgac gggcgcgtcg ccctgatcag
     1501 cgacgcgatg ccggccgcgg gcctcggcga cgggagctat tccgtctggg gcgaaacgct
     1561 cgaggtggag ggtggcgcgg cgcgtaatgc cgcgggcgcg ctcgcgggtt cgacggcgct
     1621 cctcgacgac tgcgtgcgcc gcctcgcgga aacgggcctc cccgaagagg tcgccgtgcg
     1681 cgcggcgtcc gacgtcccgc gccggatcct cgcgtccggc tagcattaac ccgccgtttc
     1741 tgagatcctc gcgccacaaa atcccatgga tctcgaaatc cgcgacgacg gacgcgtgac
     1801 gatcctcacg ctgaagggcg acctcggaat cggcgattcc gaaacgctct tcaagcggac
     1861 cgtgtcgcgc ctgctcgagg aggggaaggt ccacctcctc gtggactgca cgggcctgcg
     1921 cttcgtggac tcgacgggac tcggcgccct cgtccgggcc ctgacgacgt cgcagcacga
     1981 gggcgggcag acgaagcttc tcggcgtcgg gccgcacatg cgcaagctcc tcgaaatgac
     2041 gaagctcgat tccgtcttcg aaacgttcac ggatctcgag aaggcggtct ccagcttcta
     2101 gagtttcatg tccagcggat gggagagtcc aggtccgggc ggagccgccg acgcctcggc
     2161 gctggcggag ctggcggcct gcgaaggatt cgcccaggtc gccgcgttcg tggcgcggcg
     2221 agcggctgcg gccgcggggg ccgacgcggc gctgctcttc gctcccgacg gcgcccaggc
     2281 gggattcgtc tgcgcaggcg cctgggggga gggaacgccg aaggctctgc gccgggcggc
     2341 cccccgggac cggggcctgc tgcacgagct cctgcgcgac cgctcggccg tcctcctcgg
     2401 ccgtgaccgc ttcacccgct ccgacgacgc actcctcacg acggcgccgg cgggcgccga
     2461 ggcgtgcctt cttctccccg tggttcagga ccgcgccgtg gccgccgtcg tcgccttctt
     2521 cttccgccgg gcccccgagc cggcgcccgt gctcgcgagg acgctcccgt tcctcgaggc
     2581 cgtcgtcccg gcactcgcga aggctcgagc cgccgagcgc aaggcgtcgg ggatgctcca
     2641 ggccatcgag cgcctcacga gcctcttcga cctgtccaag gcgttcggct cgacgatcga
     2701 cctcgccgag ctcgacgcgc tgatcacccg gaaggccgca gatttctgca cctgcgaggt
     2761 ggcctcgctc tggttcctcc aggacgacga ggtcgtcctc gccgcgacgg ccgtcaaccc
     2821 gaactatgac gtcgcctccc ccccggcctc cgtgggcgcc tccgtcgtcg cgaacgtcct
     2881 cgcggacacg gaggtcctgc gccggaactc cctcccggag gacgacccga tccgcacggc
     2941 cgacccggcc taccccgttc acgcgcttct cgccgtgcgc ctcgtcgaag acgacgcgcc
     3001 ggtcggcgtt ctcgccctcg cgaacaagcg cgggcggcat ccggaattca gcgacggcga
     3061 cgaggagctg ctcgtcgatc tcgcgcgcca ggccgtgcgc gcgctccaca acgcacgcca
     3121 gtacgaggcc gagaagaagg tcgaggagct cgacgccctc ctgaccgtga gccgcgagat
     3181 cacgtccacg ctggacctcg acaaggtcat gaatgcgatc gtgaactcca ccgcggcgct
     3241 catccgctac gaccgatgcg cgatcggaat ccaggaccga ggtcgcctgc ggctcggcgc
     3301 ggtctccggg atcctcgagc tggaccgcag ccggcccgat ctgaagcgga ccaccgagat
     3361 cctcgaatgg gtctacggcg gcggggcgga cgtctcgatc gtcgagagcg aggacgggac
     3421 gctcgagacc tcgcggcccg agacgaccga gaagttccgc gcgttcttcg ccgagggaaa
     3481 gctgaagtcg ttctacggga tcctcctcaa ggacgacgaa ggtccgctcg gcgtcctcgg
     3541 gttcgagagc tccgagcgcc tcgaattcga cgaggagacg cgcagcctcc tccagatcct
     3601 cgtcaaccag gccaccgtgg ccgttcgcaa cgcccagctc taccggcagg tgcccctcgc
     3661 gggcttcctg cagccgttcc tggaaaagaa gaagaagctc ctcggcgtcc cgcgctcccg
     3721 ccgcgtcacg tggagccttg gagcgatcgg ccttctggtc gtgctcttcc tcgtgccgtg
     3781 gcggttccgg ctcacgggaa acgcgcgcgt gctccccgcg cggcgggccg ccgtcaccgc
     3841 gggcgtggac ggcgtcgtcg cccgtgtcct caagctggag ggcgactccg tgaaggcggg
     3901 agaggtcgtc gccgtgctcg agaacgaggc ctacgccgcc tccgccgccg cggcgcgagc
     3961 cgcttacgac atcgcggagg cggacgtgtc gcgcttccgc gcggcgggcg acgcggcggc
     4021 cgcgttcgag gccgagtctc accgcaagga gctcgcggcg cgcatcgcgc tcgaggacga
     4081 gcggctcgcc cgcacgcgcc tcgtctcgcc cgtcgagggc gtcgtcgtga cgccccgggt
     4141 ccaggagcgc gtcggccaga acctcgcgcg cggcgccgag ctctgcgtcg tggccgacac
     4201 gcggacgatg gtcgccgagg tcgcgatccc cgaggaggag gccgcgcgcc tccggcccgg
     4261 ccagccggcc gaggtcaagc tcaacaccta cccgggccgc acgtttgccg gctccgtcac
     4321 acgcgtcggc gccgtcgtac gcgaggaagg caaggaccgg ttcgtcgtgg ccgagatcgg
     4381 aatcgagaac gtggacggcc tcctgaaaac cggcatgcag gggcggggca aggtccgcgt
     4441 cgggtggagc agcattgcga ctctcgtcct gaggcgcccc gcccgctggc tctacgccaa
     4501 gctctggccg gtcctgccgt gagcgccgcg cccgcgtcgc ggcccggact ggccgccgag
     4561 aggggcgccg cgctcctggc cgagaagttc ggcgcgcggg gagaacggcc ctcactcaag
     4621 aagaacctcg tcgtgaggcc ccaggtccag atgggcgaga cccggatcat ggtcaaggat
     4681 ccggaccgga cgaagtacta cgtcttcgcc gactacgagt ggcgcctcat cgagttcttc
     4741 gacgggacgc gcacgcgccc cgagatcgtc gcggattacg gggtccgcta cccgttcgac
     4801 ccgatcgacc tcacgctcgt cctcgagtac gaggagatgc tgcgcaagat cgacttcatc
     4861 gagcggtccg gagcggagaa gagcctccag ctcctcgccc actccaagga ggcgcggcag
     4921 cgggcggcgg aggagaaggc cgagggcttc aaccccttct ttctcctctt ccatgtcctg
     4981 gatcccgagg aattcctgaa gcggacggtg aagtacgtcc ggtggatctg gacgccgccc
     5041 gtcgtgatcg cctggtcaat cgccgtggcc tggacgatcg gagtcttcgt ccttcactgg
     5101 gagcccatct tcacggggac ctacgagctg tatgcgttcc tgcacaagcc gttcatcgac
     5161 gcggttcatt tcttcttcat cctctcgttc ctcggcttct tccacgagta cgggcacgcc
     5221 tacgcggtga agatttacgg cggcgaggtt cacgacatcg ggctcgcgct gctctacttc
     5281 acgcccgcgt tctattgcga cacgacggac gcgctgctct tcgagaacaa gtggcaccgg
     5341 atgtgggtca acacggcggg gatctacgtg gagggcttcg tctgcgccgg ggcgaccgcg
     5401 ctgtgggtcg cgtcgtatcc cgacacgctt ctgcacgaac tcgcgtacaa gacgatgctc
     5461 ttcacgggaa tttcgacgat cttcttcaat gtgaacccgc tcgtgaagat cgacggctac
     5521 cacgccctca cgagcttcct cgagatgccc gacctgcgcg aggagtcctt ccactacctc
     5581 ggcctcgtct tccagaaaca cgtcctgcgc ctcgacgtgc aggtccccgt cacgacgacg
     5641 cggaggcgcc ggatctactg gatctacggg cccctggcac tggcctacgt cggcgtgatc
     5701 atgtcgttca tcggcggggt cctgaacaac ctctaccaca agatcgttcc ggacttcgcg
     5761 gtcgttctcc tcgtcgcctc gctcgcgttc atcttcaaga aacgcgttcg gctcgtcttc
     5821 aggacggcac gcctcctcta tctcgacaag aaggagttcc tgatgtctcg cgccgcccgg
     5881 ccccgcctcg cggccctcgc ggccgttctg ctccttctcc tcttcgtccc attcagccgg
     5941 gagacgctga cgagcgaggg cgtcctcgcc ccttccgcgc gcctgcgcgt cgaggccccc
     6001 gaggacgggc gcgtcgaggc ggtgctcgcg aaggagagcg acgcggtcgg cgccggagac
     6061 gtcctcgtcc gcatgtcgag cgctgccgcg acggccgcgg cggcggaatc cgccggcgac
     6121 cgcgagcgat cagcgggggc cgcgcgcgcc tcgcgctcgg agggcgacgc gggcagcgcc
     6181 tatcgggacg agcgcaagtc ggagtctgcg gacgtcgccc tgacgggcgc ccaggcccgc
     6241 ctcgagaggc tcgcggtgcg cagccctctc gccgggctcg tcctcacgcc gcgaaccgag
     6301 gacctcgagc accgattcgt gtcgaaggga acgatgcttc tcgaagtcgg cgactgccgc
     6361 gagctggtcg cgcccctccc ggtctccgag cggctccacg acgacctccg ggtcggagcc
     6421 gacgtgaccg cctacgtgcg gcagcggccg ctcgaggcga tccacgggac cgtccggcgc
     6481 atcgcgcccg cgacggccgg tcagccggga actgcggctc cgggcgtcga gtccctgccc
     6541 ggcgagatgc cggatcgctt cgtcgcgctc gcgttctttc cgaatcccgg ggggacgctg
     6601 cggccgggct ctcccgttcg cgcaaaaatc gccggtcctc gccgttcatg ggccgcccgc
     6661 ggcttccgaa tcctcggccg ctggatgagg accgtcttct ggtgacgctc tgaaaacacg
     6721 tcgaggggcg ctcccggccg ggaacgcccc tcgcgatctc tcgaagtgcg tcgccctact
     6781 cgtcgttgtc cgtggcgcgc gtggcacggg tcgacagggc ctcgcgggcg ctgcgggccg
     6841 acagcgtctc gcgggcgctg cgagccgaca gcgtctcgcg ggcgttgcga gccgacagcg
     6901 tctcgcgggc gttgcgggcc gacagcgtct cgcgggcatt gcgggccgac agcgtctcgc
     6961 gggcgttgcg ggccgacagc gtctcgcgcg cgctacgggc cgacatcgtc tcgcgcgcgc
     7021 tgcgggccga cagcgtcttc gcgctcgtca cggccgggct gttcccgagg ctcccgaccc
     7081 tgatctcgct catgacctga ccggtcgctg acttgatctt ggccttctgc tcgggggtca
     7141 gcttgattcg aacttcgttc gccatggttg cctccttctc ctgttttccc gcttgggtct
     7201 ccctcgcttt cgcaagagcg ctgccaggcg ccggagaggc acggtgagcg cagtaacgcg
     7261 gctttcgtgc tgcaatccgg tctcaatacc cggcgaggac cgtcgttccc gggatgactt
     7321 tttcaacccg ggttgacgga ttcaacctcc ctagaggccg tacttccgac agaggtactt
     7381 catctggttc gggtccaggc ccagggcttc cgcggcccgc gccttgacgc cgccgctgcg
     7441 agcgatggcc cgcgcgacga gctccttctc gaaccgcgca accgcggcgg cgagcggctc
     7501 gcccgtctcc gttccgggcg gcggcgccga gagattggcc gctggaagat gggcgggggt
     7561 gacggcctcc tcctcgacca tcaacgtcag catttcgacg aggtgctcca gctcccggac
     7621 gtttcccgga aacggcatcc gcttcagcgc ttcgagagcc tccggcgtga agcctttctg
     7681 tgccttgccg tggcgacgcg cggcccgcct cgagaagaac tcgacgagga gcggaatgtc
     7741 ctcgcgtcgc tcccgaaggg gcggaaggac gaggaggacc gtgttcagcc gatagaagag
     7801 gtcttcgcgg aaggcgccct tcttgacgag aagcgagagg tcttcgttcg aggccgcgac
     7861 gaggcggatg tccgcgtgga tcgtcctctt ccccccgacg tgttcgaagc tccggctctc
     7921 gagcgccctg aggagcttgg cctggacggc gggagaaagc gtcgacacct cgtcgaggaa
     7981 gagggtcccg ccgtgcgcca gctcgaatct tcccgggcgg gtggcgacgg ctccggtgaa
     8041 ggcgcccttc tcgtgcccga agagctcgga ttcggcgagc ccttcgggga gcgccgagcc
     8101 gttgacggcc acgaacgggc cgccgctccg cgggctgccc cggtgaatcg cttcggccac
     8161 gagctccttc cccgtgccgc tctccccctg cagcaggacc gtaacgtccg tgtccgcgac
     8221 cttctcgatc ttcgaaaaaa gggcccgcat cggaggactc gttccgatga tccgggggaa
     8281 cgaccggtgg cggacgactt cgttcttcag cgcttcgttc tcggccagaa tgcggctgcg
     8341 ctccagcgcc ctccggagga cgagaagcag ctcggccttg tcgaccggct tctggaagaa
     8401 gtcgtaggcg cccagctcga cggccctgag cgcccacttg cgctcggcct cgccggtcat
     8461 gacgacgatg gccgcgtcgg ggcgccgctt ccgaagggcc gtgaggagcc ggaggccctc
     8521 gtcggcctcg ctcgacggcg ggagctgaag gtcgatgagg aagaggtcga agggttcgtc
     8581 tgcgagggcg aggcccgcca tcgcgtccgg cgccgtcgcg atctcgaagg tccccttgag
     8641 ggcgaagacg agctggtcga gcaggaggcg gtcgtcctcg acgatcgcga ggcggccttc
     8701 cttcccggtc acgacccggc ctcgaccggc aggagcgcgc gcacgagcgt cccctggccc
     8761 tcgcggctct tcacgacgag gtcgccccgg tggaagagca ggatctggct gctcgtgtag
     8821 agaccgagcc cgacaccttc cggcttcgtc gtctggaagg gccggaagag tcgggtctgg
     8881 acgaactcct cgctcatccc gggtccgtcg tcctcgatct cgacgaccac ccagcgcttc
     8941 cggccgcggc gctcctcgcg cgtgcgcacg gtcacgcggc ctcccgccgg cgcgacggcc
     9001 tcgagcgcgt tctggacgac cgagagcagg gcgtccctca ggtagtgcgg gtctccccac
     9061 acgcgcggga gcggcgcgag ctcgaccgtc acaccgagcg gcccgggtcc cccggagccc
     9121 cgcacgtgcg ccagccgcag gacttcgcgg accaggtcgt tcacgtcgac ctcgaccttg
     9181 atgaggatcg agtccatgtg cgccgtccag cggtccacca tcgtgtcgag cttgtcgcgc
     9241 gttccctgca ggagctccac gacgctgcgc ttgaaatcgg ggtccccgaa gtgctcctcc
     9301 aggttctcga gcacgagacc caggcggaag cccatgttcc gcatgtcgtg gaggaggact
     9361 gaatccatcg tggagcgcag gcgccgcttc gaggggcggg ggcgcgccgg cactccggaa
     9421 ccctcagcgc gcggagggcg cgaagacgag cttcacgccg agcccgggcc gcaggacctt
     9481 cttcgcgtcg cgcgggaccc ggacgaggac ctggatcgtt ccgctcgccg gatcgacgac
     9541 cggactcacg aaggacacgc ggccggtggt gcggacgtcc gggaactccg tcgacgagac
     9601 gaccacctcg tcgttcaccc ggaccgaccc cagcgccgcg gtcggaaggt aggcgcgcgc
     9661 gaggagcggc tcgagggcca tgaccttgaa gagaggcttg tgctcgtcga ggaggacctt
     9721 ttcgcctgcg cggacgaacc ggtccgcgac gacgccgtcg aagggcgccc ggaccacgga
     9781 gcggtcgatc acgagcttcg ccctttccag ctccaccgcg gccagctcga atcgcgatct
     9841 cttctgttcg agctcggccc tgctcgcgag tccctgccgg aacagctcct cgaagcggtc
     9901 gttgtcggcc cggctcacgc gaagcgtttc ctcggccgcc tgccggtcca ggacgaactc
     9961 cctctggtcg aggacggcca gcgcctgccc tttcttgacg aggtcgccgc ggtcggccgc
    10021 gatgctgtcg acgactccgc tcacgcgcgt cgtgacggac acctcctgct cgaccatgag
    10081 gttcgacgtc gcggtcagag gccccttcgg cgcgggtgca gagcccggcg tctccgcgga
    10141 cagaggcgcc gccagaagga gcgcgatcac gagcccgttc gcgttcgtcc agaacccccg
    10201 gagtgaatct cggcgcatca tatccggaac gcccttcaga tcttccagaa ccagcccttc
    10261 ttctcgaaga ccctgaggac ggcccaccag aagaggaccg tggccagtgc gaaggcatgc
    10321 gagcgcagcg tcgggtccgc gatccacgaa aagcccgccc ggtagagcac cgtatggagg
    10381 ctgatcgtct ttccggctgc gtccgaaacc ctgatcaggc ccatcgtctt gccgagaagg
    10441 ccggagagga cgaagacgag gagcggattc gtgccgaacg tgaagaacgg cgcgaacggc
    10501 ttcttcccgc cgaggacgtc gatgacgatc acgcagaggc cgatcgcgag gagggcggcg
    10561 cccgacgtga agacgacgta cgacgccgtc cagaggttct tgttgatcgg aaggaccttg
    10621 ccccagagga ggcccgcggc cgtcgcggcg gtcccccagg ccacgaggcg tgcgaccttc
    10681 gccttcacgg tcgcctcgct cctcaggagg agcccggtca gcgttcccgt gagcagcgtg
    10741 gcgatcgccg tgagcgtcga gacgagccct tcggggtccc agccctgcgc gttcttccag
    10801 aggtggccct tcaggacggc gaggtcgacg gcgcgctgga cgttcccggc cggcgtgagg
    10861 tcgtagccgg tcccgaggag gatccacgtg tgaagcgcga ggagcagcgt gacggccgcg
    10921 gccactcctg ccgagcgccg cttccagacg gcgagcagga tccacgtgcc gaggacgaac
    10981 gtgatcccga tgcgcgggag gacgcccgga attcgcagcc gcgcgaggcg ctcgaagaac
    11041 ggcacgaacg gatagagcgc catgagccag ccgacgagga cgatcgtcat cccgcgccgg
    11101 aacgcgtgcc gcgcgaggtc gccgcggtcc gcaccctggg ccatgcgctt ttcgagcgag
    11161 aacacgcccg cgacgccgac gatgaagaga aaccacggaa agatgaggtc cgtcggcgtc
    11221 cacccgtgcc actcggcgtg gtcgaacggc cgatagagat gcccccagtc gcccgggttg
    11281 ttcacgaaca ccatcccgac gaccgtcagg ccgcggaaca tgtcgagggc ggcgaggcgc
    11341 tgctctttct cgatgaccgc atgttagaac gggggacgag gagaagaggg ggatgaagat
    11401 cttcttagaa agtgaatacg cacggccgca gcgtctttct tctcttctct tcctcttcct
    11461 aagcagcatt ctcttatctc tttcttcctg caccacgatc cccgcgtcgc tgcacgtgac
    11521 gcccgcggac gcgcccctcg cccgccgcgt cgcgaagctc gcggcgggca gcggggcgca
    11581 catggggatc gtcgccctgc acgtcgagag cgggcgccgc ttcgagctga acgcggccga
    11641 gcccttcgag gccgcgagcg tcatcaagat cgccctcctc gccgaggccg ccgcgcgcga
    11701 cgccgagggc acgctggacc tgacggaccg ctggaagctc accgcgaaga gcctcgcggc
    11761 gggctccggg atgctcgacg agttcgaccc gggcctcacg cccacgaacc gcgaccttct
    11821 gtctctcatg atcgcgatct ccgacaacac gtccgcgaac cgcttcatcg acctcttcgg
    11881 cgcggacgcc gtgaacgcgc gcatggcgaa gctcggcctc gcggacatcc gcctcgtcgg
    11941 ccgcatcccg gacctcggca aaggagaggg aaagtgggcg cccctcggcg tcatgacgcc
    12001 gaacgcgacc gccgagtact accgccgggt cgcgacgcgc tcgcttctcg acccggcctc
    12061 cgatcgcgtc attgcaaagc tcctcgccgt ccagcacacg cgcgaccggc tgccccgcct
    12121 cctcctcgac gagacctcct cgaaatgggc gggaaagacc gggagctacg gcggcgtgcg
    12181 caacgactcg ggaatcctga cgacgaagaa gggccggttc gtcctcgtcg cgttcgcgga
    12241 ccgcatcccg gacgagaaag gggcgacggc gcaggcgacg cgtgcgatgg gagagatcgc
    12301 ggcggcgatc gtgaacacct ggtcggagac cctcccggac gtcgcccccg cgctcgagcc
    12361 gccggccgtc cccgcgctcc gcccggccgt cccgcggatg gagacgacgc tcgccgaggc
    12421 gcgcgcggca acccagccgt cgagcctcgt ccgcgtctgg cgcgacacgg accggcgctt
    12481 ctgggacgcg tgggcgctcg ccggcggcga gacgcgcgac gcgtgcctga tcccgatgcc
    12541 gaacacgtgg tgggagggct tccggccctc gaagatcgag ccgatctcgg cgctcatcct
    12601 ccaccacacg tcgcaggcca cggacgagga gtgcatcgcg ctcttccaga ggcccgagag
    12661 tcgcgtctcg tcccacttcc tcgtcgggcg cgacggacgc ctctaccagt tcgtgtcgct
    12721 cgagcaccgg gcctggcacg cggggccctc gaccctccac gggcgcctcg cgctcaaccg
    12781 gacgtccgtc ggcgtcgaga tcacgggcga cggcaaccgg tacccgttca cgccggcgca
    12841 gatcgagacg gtcgttcgcc tcgtcggcgt cctgacggcg atgttcgaca tcccggcgcc
    12901 gtggatcgcg ggccatcagc acatcgcgcc tgaccggaag gacgatccgg gcgtcctgtt
    12961 cccgtggaac gacgtcgtcc gccgcggact cgaactgtcc gagtccctca gaaagaaggg
    13021 ccttccaaag ggggattgat ttcccccggg cggtctctta taggatgcgc ccgcccatca
    13081 tgcacggcag actttcccgg ctcgacgacg ccgagactct gcgcgggttc gtccgggatc
    13141 tccgggaggg gatctacgtc acggaccgtg acggccgagt cctcgacgcg aacggggcgt
    13201 ttctcgcgat gttcggcgtg tcctcgctcg acgagctccg ggcctcgagg gccgcggacc
    13261 tcgtcgtcga cgtcgcgcgc cgcgaggccg agctcgcgct cctccaccgc gacggcgccg
    13321 tccgcgactt cgagctggag atccgccgcg cggggggcga ggtccggacc gtcatcgact
    13381 cggccacgtg cgtcacggac ccggagaccg gcgaagtctt ctaccacggg atcctgatcg
    13441 acatcacgga ccgcaagctc ctcgagcagc gcctcctcga ccagagcctg cgcgacccgc
    13501 tcacgggctg cttcaaccgg cgctacctca acacgttcga gcgccgacac gcgagcggat
    13561 cccattcgtc cttctcgggc gcgtggggct gcatcatggt cgacctcgac tacttcaagc
    13621 agtacaacga cgagttcggg cacgaggcgg gcgacgccgt cctgaaccgc gtcgcgcggt
    13681 tcctcatgcg tcagacgcgc gccgaagagt cggccgtccg catgggcggc gacgagttcc
    13741 tcctccttct cgacggcgcg gacgcggccg cgacggagtt cgcggcccgg cggctgcagt
    13801 ccaccgcgga ccgcgagctc ctcgtcccgt tctccatggg ctgggccgcg cgggaggacg
    13861 gcgagaccct cgagcagacg atcgcgcgcg cggaccaggc tctcctcaag tcgcgcggcc
    13921 gcacgcgctc gggcgaacgg ccgcggctcg tgctccctgc ctaggcctga accggtgtga
    13981 ctccgagtcc ggggccttcc ggcatcgccc agacgccctt gtcgatcctg agcccgtgga
    14041 acgggtcctt cgcgagcagg aggttcccgt cgaggtccag ccagtcgaag agcgggccca
    14101 gggccagccc cgcggcgatc ccgacggagg actcgatcat gcagccgcac atcgtcttca
    14161 ggccgacggc tcgcgcgacg acgacctgct cccacgagcg cgtgatgccg cccgccttcg
    14221 cgagcttcac aacgacgccg tcgaagagcc ccgccttctt ggcgacgtcc gctgccgtca
    14281 ggacgccctc gtccccgagc acggggtacg tcgcgagccc ccggagcttc accatctcgc
    14341 cgtccagagc ggcgggcatc ggctgctcga gatagagcgg cttgacgccg ttcagccaga
    14401 ggatcttcct gcgggcctcg tcgaccgtcg cccagccctc gttcgcgtcg accgtgaggg
    14461 cctggctcga gacggaccgg atcgccgcga tgttctcctc gtcgctcggg aggccgatct
    14521 tgatcttgag cttcgggaac gccgccgctt ccttcgtctt ctgcttcatg acctcggccg
    14581 tgtcgatgcc gatcgagaac gtcgtcacgg gcattcccga ggacggcacg ccgaggagct
    14641 ggtggacggg gcggccgagc cgcttgccct tccagtccca cagagcgatg tcgagcgccg
    14701 cgacgacctc gtggtcgttt ccggcggcgg cctcggcctt ctcgagccat gtcaggtggt
    14761 cccacggcga cagtccctgg acggccttct cgaccttcgc atacgcggcc tcggacgtct
    14821 cgaaactctg gttccagcgc ttgttcgcgg ccgcttcccc gtaacccgtc acgccctcgg
    14881 ccgtgatcgt gaggaggccg ttcttcttct cgtccgacga gccgcgggcg atcgtccacg
    14941 tgtgcgcgag cttcaggacg agcgggcgat aggagaggga gaccggggcg gaagacgggc
    15001 gggagggagc ttccgcgaag agcgcctccg gaagggcgga agaggcggcg gcggcggccg
    15061 acgagagaat gagtccgcgg cgggaaatgg gctgcgtcat gcgccgatta tggtggaatc
    15121 ccgggatgcg tttcgaagga agacccgcct gccgcacggc cgccgctcgc gccctcgccg
    15181 cggcccttct tctcttctca ttcgaggaac cctctctctc cgcgtctccc gacgcgacgc
    15241 ccgcgcccgc gaagacggcc gtcgtcgtcg cggcggtcca ggacctctac gtgaggcccg
    15301 acgagacgtc gagcgtggac gaccaggcga ttctcggcga ccgcgtgacc gtcctcgagg
    15361 atgccgcggg attcgcgaag gtgaagacgg ccgcgggcga gatcgcgtgg attcccgagc
    15421 gcgcgctgcg ccgcggcgcc ggcgcggacg cgccggcaca caaggtcgct cgggtgacgt
    15481 cgaacttcgc gcacgtctac ccgtggccgt ctttcacgaa gcagaagccg ctcctcacgg
    15541 ccccggtcgg ggccacgatg gctttttcgg atttcttaga aggtaaagag ggcggggcca
    15601 gcgatgccta ttcctgggtg aaggtcagct tgcctgacgg gcgcgaagga tacgtatcag
    15661 cccgggacgt cgagtggggc gccgccgaca cacccccttc taagaaatct tcgtcttcat
    15721 ccccgtcttc ctggattgcg ttcggaaagc gcttcctcgg cgcgccgtac acctggggcg
    15781 ggacgacgcc gctcggtttc gactgctcgg ggctgatgac ccggatcttc tcggagcacg
    15841 gggtgacgct ccggcgcaac agctacgaac aggcgttcca ggatccgcag ctcgtccccg
    15901 tcgccttcga gaggctccgg ccgggcgacc tcctcttctt cggcacggat gacaagatcg
    15961 accacgaggc gatgtggatc ggcgacggca ccgtcctcca gtccacgcgg cacaacgtgc
    16021 cgggcgtgca ggtcacgtcc ttcgactccc cttacttgaa gcctctgttt cggtacgcca
    16081 gacgactaaa gaagattttc ttagaaggtg aagaggggcc gaggctgacc caggcgaagg
    16141 tgcgcgacct gcagcgcgcg ctcgactcga ttgcggacgg gagcggggct cgcttcggca
    16201 tctacttcaa agatctgacg accggccaga ccctttctaa gaactcttcg gtctctttcc
    16261 acgccgcatc cacgatgaag acacccgtcc tcctcgagct gctgcgccgg gtggacaacg
    16321 gaaccgttcg actctccgac gagctgcccg tgaagaacga gttcaagagc ctcgtcgacg
    16381 gctcgccgtt ctcgatcggc ctcgaggaca acgacggccc cacggtcgcc tacgccggga
    16441 agacggccac gctcgagttt ctcgcgcgcg agatgatcgt ccgctcgtcg aacctcgcga
    16501 cgaacctcct cctgacgctc ctcggccccg cggaggtcca gaagttcacg gacgcgctcg
    16561 gggcgccgac cgtccacgtc cgacgctgcg tcgaggacac caaggcgttc gagaaaggca
    16621 tgaacaacga gacggacgcg gccggaatgg gcaccgtcat ggaagccgtc gtccggacgc
    16681 caaagctttc ggcggccgcg aaggcgaagg cctgggagat cctggtcggc cagacgttca
    16741 acgaggaaat cccggcgggc ctgcaccccc agtccggcgc ggtcgtcgcg cacaagaccg
    16801 gctcgatctc gtcggtccag cacgacgccg ccgtcgtccg cctgccggac gggcgggaat
    16861 acgtcctcgt cctcctcgcg aacgacttcg gagcgaacga gaagggccgg gagcgcgtca
    16921 acgccgcggc gcggaagatg tcccgcgcgg tctgggaggc gatgatcgcc ccgtgaaacg
    16981 cggcgtcgtc cccgcgctcc tcgtcatcta cgtcgtctgg ggttccacgt acctcgcgat
    17041 ccgctggggg ctcgagacga tcccgccctt cacgctgtcg tgcagccggt acctcgtcgc
    17101 ggggaccgcc ctcctcctgt tcgtgaaggc ccgcggcgcg ccgcgcgtcg ccctgcgcga
    17161 cctcggcccg gcgttcctca cgggcgggct gatgctcctc tgcggcaacg gcggcgtcgt
    17221 ctgggccgaa cagcggatcg cgtccggcct cgcggcgctt ctcgtcgccg tcgagcctct
    17281 cttcatcgtc ctcctccagg cgacgctgcc gcaggagcgc cgccgcccgt cgggccgcgc
    17341 tctcgtgggc gtcgcgctcg ggatcgcggg cgtcatcctc ctcgtgggac cggtcggcct
    17401 cgggggcgcg cgtgtggacc tcgcgggcgc cgcggccgtc ctgttcgcgg ccttcgcgtg
    17461 ggcgctcggc tccctcctct cgcgccactt gaagccctcg gtttcgcctc tccaggcgac
    17521 cgccctccag atgctcgcgg gcggcgtcct cctcggcggc gcctcgggcc tcgcgggcga
    17581 gtgggcgcgc ttctcgccgg gcctcgtttc cgcgaaatcc gtcgcggcgg tcgcctacct
    17641 cgtcgtcttc ggctcgctcg tcgcgttcac ggcctacgtc tggctgctgc gcgcggcatc
    17701 gccggcgctc gtctcgacgt acgcgttcgt gaacccggtc gtcgccgtgt tcctgggctg
    17761 gctgctcgcg gccgagcccg tctcggcgcg gacgctcgtc gccgccgccg tgatcgtcgg
    17821 ggccgtcgtc ctgatcacgc tcggcgaatc acgccggtga tttcctgaga tagaatctcc
    17881 gtaccaaccg gaaaagaagg aaaaagagga gattcgatga agaagacctt tgtggtgttt
    17941 gccctcgcgt gtgtcgccct ctcggccggc gccgtcctcg cagccgacgc ggcgcccaag
    18001 cacgggctcg cgaccgtcga cagcgcctgg gtcaaggcga tgctcgcgaa cgacgccgcg
    18061 gcgtgcgccg ccctctacgc cgacgacgcc gtcctcgtcc tccccggctc gggggcgatc
    18121 aagggcaaaa aggcgatcgc ggacgcgtac gcgggctggc tcagccaggt caaggtgacc
    18181 gaggcggccg tcatggacaa ccactaccgc tcgtccggca aggtctcggc gggctggggc
    18241 gccttcaagg tcacgaccgt gccgaaggcg ggcggcgcgc cgacgaccga ggtcgggacg
    18301 tggtgcgccg tcgccgccca gcagaaggac ggctcctgga agtacgtcgc cgaccacgcc
    18361 tccgacgacc ccgcgccggc caagaagtag ccgctactcg atcgactcgc cgctccgcgc
    18421 cttcgcgagg tgcggggcgg cgccgtcggg ccacgtgagc gagccccgcg cctgcgacgc
    18481 gagcttgccg tccgcggcga agaacatcgt ctcgttcggg cgcttcgagt tgagcttcgt
    18541 ggtcgtgtcg ccagacgggt cgagcacgag cgtctgcgga aacggcggca cgaggttctt
    18601 ctccttcagc caggcgtcca gcgtggcgaa gtccttgtcg tgcgcgatga ggacgaactc
    18661 cacgtcccgg tcccccttca gggcctcctg ctggaggaag atgccggcga gctcgtcgga
    18721 ggcgccgggc gccgtcgtcg agaagaagtg caggacgagc gcgtggcccg gcttcccgac
    18781 gaggttgcgg ctcttgccgt cgcgcgtctc gacgacgagg cggccgcccg cgtcgaggtt
    18841 caggatcccg tggtcgtggt cggggtgcgt ggccggcgtg agcgacagcc agtaggcgta
    18901 gcccatggcg gcggcgacga atgcgccggc ggcgaggaga acggagcggc ggcgggaagg
    18961 ggtcatggtc tgctcccggc gtccgcctcc gggcggacgt ccgtttcggt ttcgacgttg
    19021 aaacgcctca ggttcgtgaa cgtcgcccgg aacgacgtgt cgagacggaa gaagagcgcg
    19081 gtcttgccgg tcacgcggaa gtcgacggcg cacgggaacc agaggccccc cccggcgggc
    19141 tggcccgtgt acgacacgtc cgcttccttc acgttggccg cgaagcctcc cgcgaccttc
    19201 aggggccggg tcaggcgggc ctcgaccgac cggacctgga acgtggaggc atcgatcacg
    19261 gcgcggccct cgagggcgtt cagcgcgcgg tcgcccaggc tcttcgcgga cggcgccgtg
    19321 gggctcggag agaagtcgag gacgtaggcg ggccgcccct cgatgacctc ttcgcccacg
    19381 agccggatcg tataacggcc gatgagctcc gaaaggcggc gcgagcccac gagcgggtcg
    19441 tcctcgtcgc cggcgacgcg cggcggagcc ttcgccgccg cctcggcctt ctcctcgacg
    19501 cggcgtctgc ggcccttctc gtccttctcg gccgctttcc tcttctcgtc ctccgtcgcc
    19561 gggcggccgt cgacctcgac gagctcccgg gagctttccg cgccgttctc gcccgcaaag
    19621 acgtagaaga ggcgcgattc gaccttctcg gggcggccct tcgcccccca cgtgatctgg
    19681 acctcgcgct gatcgaacgt cgcgccctcg aaggcggtct cgacccgccg ctggctcttc
    19741 acgacgcgcc ggatcagctc gacgggatcc acgtcggtag cgcccgcggg ctcccgggcc
    19801 atcgcgagcg cgaatccggc ggcggtcaga cggcggatca gtctcaccag ggaactatac
    19861 ggcggagccc cccggcccga tttcggtacg atcgggggac aaggagagcg gatgcgcgtc
    19921 atcgtcaccg gcgggaccgg cttcatcggg ccgaagctgt gcgagcgcct cgtcgcgaag
    19981 ggtcacgacg tgatcgccct gacccgggac gcgtcgcgct cgcgcgacca cgtgcacccg
    20041 aaggtgcgcg tcgtctcgtg ggccgagggc gcggcatggg aagggttcgt cgacggcgcc
    20101 ggcgccctcg tgaacctcgc cggcgagacc atcgcccagc gctggaccgc ctccgccaag
    20161 gagcgcatcg cgaagagccg cctgaacgcc gccgcccggc tgagagccgc tgcggagaag
    20221 gccgcgcaga agcccggagt gctcgtgaac gcttccgcgg tcggctatta cggcccgcac
    20281 ggcgacgaga tcctcgacga gaccgcaccc gccggaaacg acttcctcgc gcggctctgc
    20341 gtcgactggg aggccgcggc ccgcgccttc gagccgctgg gcacccgcgt cgcgatgctc
    20401 cggacgggcg tcgtcctcgg cgcgggcggt ggcgccctcg cgaagatgct cccgccgttc
    20461 aaggcgttcg cgggcggccc gctcggcagc ggccgccagt ggatgtcgtg gatccacgag
    20521 gccgacctcg tcgagatgta cgtctgggcc gccgagaacc ccgccgtcgc cgggcccgtc
    20581 aacgcgacgg ccccgaaacc cgtcacgatg cgcgatttcg cgaccgcgct cggcaaggcc
    20641 ctgcatcgcc ccagcttcat gccggcgcct gccgccgcga tccggctcgc gctcggcgag
    20701 atggcgacgg tcgtcctcga cggccagcgc gtcgtgccga agaaagctct cgatctcggc
    20761 ttcgcgttcc gcttcccgga agtcctcccg gcgctgcagg acgtcgtagg cgactgatgg
    20821 cgctcgtcgg ccgcagcgtc ccccgcccgg acggacccgc gaaggtcacg gggcgggcgc
    20881 gctacgtcga cgacgttgcc gtgccgggga ctctggtggg cgccacgctg cgctcgacgg
    20941 tcgcgcgcgg gcggctgcgc ggcatcgtga gagacccgtc gttcgactgg gccggcctca
    21001 cggtggtcac ggcggagaac gtcccggcga acgtcgtcgc tctcatcgag gaggaccagc
    21061 ccgtcctggc cgccgacgac atccggcacg cgggcgagcc gctcgcgctc gtggcgggcg
    21121 aggacgcgct gagggtccag cgggcgctcc agcacctcag ggccgacgtc gaaccgctga
    21181 ctcccgtcct cacggtcaag gacgcgctcg cccgcggcgc gcagatctac gggtccgaca
    21241 acgtcttccg aacggtccac atcgcgaagg gcgacgcggc ggctgcgatc gcggcctgcc
    21301 ccgtccagct ctcggggacg tacaccacgc cctggcagga gcacatctac ctcgagccgc
    21361 agggcgtcct cgcgttctgg gacgagaacg gcggtgcgca cgtcctgtcc tcgtgccagt
    21421 gcccgtacta catccacaag gggatcaagc gcacgttcgg tctgacggac gagcgcgtcg
    21481 acgtcacgca ggcggtcacg ggcggtggct tcggcggcaa ggaggagtac ccgacgatcc
    21541 tcgcgctcca cgcccttctc ctcgccaagg cgagcggccg gcccgtgaag atgatctacg
    21601 accgcaagga ggacatcgag gcgacgacga agcgccaccc ctcctccgtc tcgatcacgt
    21661 cgggctgcga ccgcgacggg acgctccgcg cggtcacgat ccgcatcgtc ttcgacggcg
    21721 gcgcgtacgt aacgctctcg cccgtcgtcc tctcgcgcgg cgcgctccac gcggcgggcg
    21781 cgtaccggtg ggagcacgtc gcgatcgaag cgcaggccgt cgccacgaac acgccgccga
    21841 acggcgcctt ccggggattc ggcgcgcccc agacgatctg ggcgatggag cggcacatgg
    21901 accggatcgc gcgcacgctg cgcctcgacc cgcttctcct gcgcgagaag aacctcctgc
    21961 gcgtcgggga cacgacgccg accggccagt tcctgaagga gtccgtcggg gtcgcccggt
    22021 gcgtcgagga ggcgaagaag gcgagccgat gggaggaacg gcgtgcgtcg cccgcgcccg
    22081 cggcctccgg catgttccgg cttccgccgc ccggcgccgt gacggcgcgc aaggcgcgag
    22141 gcatcggcgc gagcgtctac cttcacggcg ccggcttcac cggctccggc gagctcgcgc
    22201 accgtgcgcg tatcgcggtc gacctcctcg cggacccgaa aacgggccgg gcccgattcc
    22261 gcgtccgcgg cggctcgacg gacatcggac agggcacgga gacggtcttc cggcagatcg
    22321 cggccgaggc cctctgggtc tcgctggacc gcgtcgagtt cgcgacgccg tcgacggcag
    22381 ccgtgcccga ctccgggccg acggtcgcgt cccgcaccgt gatggtcgtg ggcggcgtcc
    22441 tcgaggccgc ggcccggtcg ctcttcgcgc agatcgaggg cgagcgacag cagaagggcg
    22501 gctcgttcga cgacgcggcc cgacgcctgc tcgcggaccg cgggacggtc accgtcgaac
    22561 gggagtacga gccgccgccc ggactcgcgt gggacgacgc cacgtaccgc ggcgacgcgt
    22621 accccgtgta cggctggggc tgcgcgatcg ccgaggtcga ggtggacctc gacacgttcg
    22681 agacgagcgt gctcggcctc tggagcgcgc acgacgtcgg caaggcgatc cacccgacca
    22741 actgcaaggg ccagatcgag ggcggtctcc tgcaggcgat cgggtgggcg ctccaggaaa
    22801 acctcgtcct gcgcgagggc cgcgtcgtga acgcccgact gacggactac gtgatcccga
    22861 cgatcctcga cgccccgcga ttcacgacgc tcctcgtcga ggatccgttc tcgaggggtc
    22921 ccggcggagg cgcgaaaggc ctcggcgaga tcccgatgga cggcggcgct cctgcggtcg
    22981 ccgcggccgt cgagcacgcg acggggatcg tctgcgacga ccttccgctc ctgcccgaga
    23041 cgctcttcgc gctctcgaag gaggcccgga agtgaacgtg cgcttcaccc tcaacgcgcg
    23101 gcccgcgacg gtcgacgcgc cgccggccgc gcggctcctc gacgtcctgc gcggggtgct
    23161 cggcctgacg ggcacgaagg agggctgcgg cgagggcgaa tgcgggtcgt gcaccgtcct
    23221 cctcgacggc gcccccgtca atgcgtgcct cgtcctcttc gggcaggtcg agggccgcgc
    23281 cgtgacgacc gtcgagggtc tgagcgaccc cgcgagcctc tcgcccctcc aggaggcgtt
    23341 cgtgaaggag ggcggggcgc agtgcgggat ctgcacgccc ggcttcctca tgtcggccga
    23401 agcgctcctt gccgccatcc cgcagccggg cgagacggac atccgcgagg cgctcgcggg
    23461 caacctctgc cgctgcaccg gctaccagcg catcgtcgag gccgtccgcg cggccgcaaa
    23521 ggagaagacg tcgcgtgagc ctggctgagc tgcccctcca cgccttcgag cggcccgcgt
    23581 cgctcgcgga cgcctgcgcg acgctctcgc gccgcgcagg caagggcgag gagacggtcc
    23641 tcgtcgcggg cggtacggac tgggtcgtcg cccaacggct cgcgccgctc tcgctcggag
    23701 gcgagctgcc gctcgtgctc gacatttcgc gcgtccccga gctcctcggc gtgaggatcg
    23761 cgggcgagcg cgccgcgatc ggcgccgcgg cgacgtactt cgagctgcgc caccatccgg
    23821 ttctcgggcg ccgcgtcccc atgctcaccg cgatggcgcg cgacgtcggg gccctgcaga
    23881 tccaggcgcg gggcacgctc ggcggcaacc tcgcaaccgc gtcgcccgcc gccgacggga
    23941 gcgcggcgct catggcgctc gacgcggacg tcgtcgtcgc aagcgtccac ggcgagcggc
    24001 gggtcccgat cgggcagtgg ttcacgggct accggcgcac ggcgcgcgcc gccgacgaga
    24061 tcatcgcgcg ggtcgagttc accctgccga agaccggcgc acaccagctg tggcgcaagg
    24121 tcgggacgcg cgcggcgcag tcgatctcga aggtcgcgct cgcggccgtc ggggaatacg
    24181 agcgaggccg ctgcgtgagg gtcgggctcg gcatgggctc cgtggcgccc gtgacggtcg
    24241 cgctgcccgc cgcgcgcgct ctcgcggtct cgcccggccc gctcgccgtg cccgacgacg
    24301 tcctcgacgc cgccgtgcgg cgcgacatct ccccgatcga cgacgtccgg tcaaccgcgg
    24361 cctaccgggc gcacgtcgcg tccgcgctcg tgcgcggctt cttccggacg ctgcgccccg
    24421 tctcgaacgc cggctgagcg ccccgctcta gatgtagctc gtccaggccg cgcggctgcg
    24481 gctcttgaag tccccgtagc ggacgcacgg aacgtagagc gcgagcacga cggcgatcca
    24541 aagcgcgtac acggccggca gtccgacgcc gaagccttga gggggcggga cgatgaacgc
    24601 cggcgactgg aacgccgccc agtgcacgag gggccccgag agcccgcccg actgcgcggc
    24661 cgcgacggcg accgcgagag tgtgggcgat cggaaggtgc gcgacgtaga agaacatcgg
    24721 gacccgcccg taggcggcga ggaagcggcc cgcggggccc ggcgcgcggt cgaggacggc
    24781 gagcgtcagg aggagaggcc cgagcgtcat gaggagaaag cacagggacg gcgggtactt
    24841 ctcgcagttc aggaacgccg cgaacgtcat cacggcggac ttctgcgggg cccaggcgcg
    24901 cgggtcgccg taggcgttcg cgagccggag gacgacgaat gccgtcgtca gcgcgaagcc
    24961 gagagcgaac gtgagccggc gccgttcgtc ccgcggcctc ccgaggaggg cgccgaatcc
    25021 gtacccgagc gccatgacgc ccggccacgg gatcgccgga taccccacct cgaggttgaa
    25081 cagacccggc agcgggaagc ccgccggcgg cgttccgaag ggaatcatcc cgcccgcgtg
    25141 gaggatcttc caggcggcgc ccgcgaggcc cgtgaggggc agcttgtcga cgaaatcgag
    25201 tgcgttgtgg cccgcgacga ggaccgcgcc gacgcccgcg atcgccctcg gcccgagccg
    25261 cagcttgagg aggaacgcga ggacgagcat cgcgacgccg agcgaccaga gcgtggcgat
    25321 gaaggcgacg ggccagagat tgaagagcca gacgaatcgg aggacgaaga actcgagcgc
    25381 gatgaggaag aggccgcgcg acgcgaggaa acgcgcgagg ctctccggcg tcttcccgcg
    25441 cgaaagggac aggaacgcgg cggttcccgc gaagaagctg aagccgggcg cgcagaagtg
    25501 ggtgaaccaa cgcgtgaaga agagcgccgg gaacgtctgc gacatgtcga ccgggtcgaa
    25561 cgggatgttc gtgacgtagt cccgcacgtg gtcgagcgcc atcacgacga tgatcgcgcc
    25621 ccgcaggacg tcgagcgcct caagaaagac ccgaaactga aggtcctgga ag
//
LOCUS       DAOMFY010000026        25360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_630_length_25360_cov_8.438490, whole genome shotgun sequence.
ACCESSION   DAOMFY010000026 DAOMFY010000000
VERSION     DAOMFY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25360
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_630_length_25360_cov_8.438490"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLB01_04030"
     CDS             complement(<1..434)
                     /locus_tag="PLB01_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQQ76500.1"
                     /translation="MPLYEYECAKCGERIEVIRSFSDPPLVVCPECGGALTKLLSSPA
                     FQFKGSGFYLTDYGKSGSAGDRAGEKSEEKKEKDKDKEKSSSKGEEGKTSSAPGGGEA
                     AGGGGSDSSKGKKDGGDGGAPGAATSSPTAPAPPAPSKKKSG"
     gene            complement(466..1275)
                     /locus_tag="PLB01_04035"
     CDS             complement(466..1275)
                     /locus_tag="PLB01_04035"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HQQ76501.1"
                     /translation="MEDRFQYTGDLAQEPLPEVLRTIHFYRVPGVLTVESGNVTKRVY
                     LLGGNIIFATSTDRSESLGEYLRRSALISSGELKASVGKLDLAEGQGRRHGELLVEMG
                     ILSDAQLRQIVTEQVKALLYAAFSWEKGSVTFEVGRYKTDELIRLDVPTSQAIVDGIR
                     RMPDPRRCVARLGPSWTIFERAEAPPEARDVSFTAAEVTLLSQVDGRRTLRDLITMGP
                     GDLGANAKLLYAFWVLRLITRRESQTSGIRRIQWKTPGGIPTAGGDGDGDG"
     gene            1330..2541
                     /locus_tag="PLB01_04040"
     CDS             1330..2541
                     /locus_tag="PLB01_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQQ76502.1"
                     /translation="MIAFEEALALVLEAAAPLPACEVPLPEAVSRVLAEDVRADLPVP
                     GFDTTAMDGWAVRAAEAVRAPTLLAVAGASGAGSVPGKLPPGSAWKVMTGAPMPPGSD
                     AVVPVEDAADVEGGSVRLDAVPKSGAHIRRRGEVYEAGALLLAAGRRLTPADLVLAAA
                     AGREKLRVARAPRAGVLVTGDELVPPGTQPGPGELRNTNGPLLLAALRRAGADVVSLG
                     VAPDDEAALARALRDALIAKFDVLLTTGGVSAGDYDFVGKALAAEGARVRFHKVSIRP
                     AKPVLFATKGPTLVFGLPGNPVSAAVAFDFFVRPALRAAAGLLPPLPPAVPARLTARV
                     KNKGPRLAFHPALLAWDAGRLCAEPLATRGSHDILAHAQADAFLELAPESSWEAGDSV
                     PVHRGTAESTF"
     gene            complement(2538..3125)
                     /locus_tag="PLB01_04045"
     CDS             complement(2538..3125)
                     /locus_tag="PLB01_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ76503.1"
                     /translation="MAAGSPGPVVPDRYLEALGGDDPVAAMAEAPDLLRRLVRGLTEK
                     QLATRPAPGKWSIKEIVAHLADNEVVLGGRYRFIAAMDRPPLPGFDQDAFVANLGVGK
                     AATGDLLDDFAMARAVNIGLLERVGESAWNRVGIHAERGEESLGRLVTMYAGHDRIHL
                     AQVETIRTGLFPKAGRGAKRKAAAKKASPKKAKRR"
     gene            complement(3143..3778)
                     /locus_tag="PLB01_04050"
     CDS             complement(3143..3778)
                     /locus_tag="PLB01_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76504.1"
                     /translation="MGTVSSPSNPRQPGSRAPSGATPAGGSPPLAGPGPNTTSRFRFG
                     EDKPVSMGPSGTAIVLTILAVAVGAWLYFKAPSTVGEQSLQRYMGFEANMESVVMRNC
                     PPPGCAAVYLTPTVGRKSQDALQGAIALSARLAEEGIECYIVLAGEPIADAVKRARGI
                     RRPVVFDPGGDWGHDSGIEKAPYWIAWRTGGKIRLRSSEPVSAGDVASAIR"
     gene            complement(3860..5839)
                     /locus_tag="PLB01_04055"
     CDS             complement(3860..5839)
                     /locus_tag="PLB01_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HQQ76505.1"
                     /translation="MSTNFATHESPRREGRALVWALAIVALVLLSLAAGLKAEAKEWN
                     AKATRTETFNASGNLKSLAVETVNGKVEIVAGTAFRADVDVTAWGATDGDAKKNLGDV
                     KVRFENENGNLSLYTEEPGVTVRHSGRGWNVRSRSDDQWRSETKYRITVPASMSVQVS
                     AVNGGVSVTGVAAPVELTTVNGRIVLEGGRRDAKLNTVNGAIVAGFAELPKGSVLDVR
                     AVNGSISMTLPAQAGFRLEGHTMSGEILSSFSLPTPAPEAVRENDEMRAERERIRAEQ
                     RKLRAEIRKKEKDQAKVRKDSGEEDIVIDLSELNEAMAELNREMAGLSQEISRSITVN
                     LNRAYEGAVGDGGATVRVSNLNGKILILAEGTTEAQAKRLTSPRSTHVVTVPPMPSLP
                     RIVVREHPMPEPAPAPRAVPAPPAPPAAGVAPVAPVAPIPPDPWGRSITVGDVAGDYT
                     PAIASGDVTVGKVSGRVTIASRSGQIRVREAGKGAEVSTAGGDIRVEAVTGDLKATTF
                     GGDIRAGSVSGDVRLETSGGDVVVRSAGGAVTARTGGGDVVLKKVRGPVTARTSGGSI
                     TCEITSTGGAAGELATSGGDVTVTLPANVKADVEIVVSGGGVDADSIASQFPEISVAR
                     RSGHILGEGRLNGGGPKLVIRSTSGVVTLKKGPAA"
     gene            complement(5874..6887)
                     /locus_tag="PLB01_04060"
     CDS             complement(5874..6887)
                     /locus_tag="PLB01_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQQ76506.1"
                     /translation="MECRESLKVREFVLARLAGESAGDGAAALAAHVAGCAACSAIAA
                     DMESVWARLGDDDASDLVASPAFEARTTAALAAETRRATNVRPFAPRGAESPLLKVAA
                     VLVAGVAGFFLARASGGGSLPGSTAFQPTPAPFRGGENVSLVSNRTLDASRSALDLSG
                     KPRLANVAYAPADAAGRVAVSFDVTTRYTVVGKPEDQAIANLLVSLMSGSSGSEGAKG
                     KALDLVSEGTRGGGAVSPEIVTLLRRTLETDKNPGVRKKAAEALAQMPPTPETRDALA
                     AALKNDVNPAVRILAVEGLAKAAAVLKDASSIESLRQKAADDRENGYVRSQAALALSK
                     IEI"
     gene            complement(6866..7489)
                     /locus_tag="PLB01_04065"
     CDS             complement(6866..7489)
                     /locus_tag="PLB01_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQQ76507.1"
                     /translation="MEAVAREREGCVEPDGISPDQEADLEALEQVRRGDEAGATRLFQ
                     RWSGPVLKFTGRMLGNAADAEEVTQDVFLKVIARANQYDGRASVASWLFAIAANACRD
                     RLRRSTRRPTVPLEAVAETAQPEIAVDLRLVERQRRAAVRRALSRLSEEQREVLVLAR
                     YHGLPYAEIARSLSISEGAVKTRIFRAMETLKTLFSEGETPWNAASH"
     gene            complement(7541..8323)
                     /locus_tag="PLB01_04070"
     CDS             complement(7541..8323)
                     /locus_tag="PLB01_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HQQ76508.1"
                     /translation="MRLTAPTLALLAASLPAAAVLPSRDVAKTLPLEAGESVRVETYK
                     GTVKVTTWDRSEVAVEARIEADDNCGSPKDMARWVDQTRVVIEKRHGGVSITSDYDAL
                     EEATSWFGRCTARPFVRYTIHMPKSAPLRIKDYKSDLVVKELAADLDVNTYKGHLDVV
                     GLSGGLRVETYKGEVNVGIVRLSGDVNAETYKGSIVLHLPGTAGFELSAESGRGSLNV
                     EFPAETGRGSHRRGLVARGPVNGGGPRVSLRTDKGSLALRKG"
     gene            8540..9472
                     /locus_tag="PLB01_04075"
     CDS             8540..9472
                     /locus_tag="PLB01_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQQ76509.1"
                     /translation="MHSLPVWIAFNVFVLAMLALDLGVFHRKAHSVSLKEAGIWSAVW
                     IALALAFGAGLWHFEGKDPALQYLTGYLIEKSLSVDNIFVIALIFTYFAVPAAYQHRV
                     LFWGILGALLMRGALIAAGAFLLSKFHFLVWIFGAFLIVTGIKMALHDNAELHPEKNP
                     LVRLARKIFRVTNDYEGQNFFVRRDGALWATPLFLVLLVVESMDLVFAVDSVPAIFAV
                     TSDPFLVYTSNVFAILGLRSLYFLLAGIMGRFVYLKLGLSVILVFVGLKMAFVELVKV
                     PVGISLGVIGLVLAVSVVASLVKTSPASRKGASS"
     gene            9469..10596
                     /locus_tag="PLB01_04080"
     CDS             9469..10596
                     /locus_tag="PLB01_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQQ76510.1"
                     /translation="MTSATRIATVAGIEIKIHVTFGIILVIGAVQWGIPHGAAGAAFG
                     AALMAALFFCVVLHELGHSLVARGFGLPVKEIVLLPIGGVARMEKNPEKPVHELLISV
                     AGPLVNVAIAGVLLAAVGARFGPLDAAGLAARLKGAPSVETALLWLLSVNVMLFVFNM
                     IPAFPMDGGRVLRALLWMVMGFGPATRIATLVGQVAAVGLGLWGLLSGNLFLALIAVF
                     VFLGAGQERADEQARVVLGTLRVGDAYNKHAIALAPGDTVGSVADYILTSYQPDFAVL
                     QGGALLGVVTRADVLQSLATETHDSYVTGVMKRDVLRVEADAPLDSVRRLLVEKGERV
                     AAVTRGGTYLGLVSLEDIAEALLVVAFRAAQDTRRKAVETA"
     gene            complement(10600..11907)
                     /locus_tag="PLB01_04085"
     CDS             complement(10600..11907)
                     /locus_tag="PLB01_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQQ76511.1"
                     /translation="MNDWKERFSWKISSIEDAIRTIPPGRRILIGSGAGEPQKLVEGL
                     VEHGTHLRNNEIVHLMTLGKAPYVAPGLEQRFRHMAFFIGANVRGAVQEGRADFLPVF
                     LSEIPTLISTGRVGIDVALIQVSPPDRHGYVSLGVSVDIVRAAVDTAPLVLAEVNPQM
                     PRTLGDSFLHVDRIAHLIPVDEPLLELASEAPDDVCRQIGLHAASLIPDGACLQMGIG
                     RIPDAVISALADRNDLGIHTEMMSDGAMRLIQSGVVTGRRKKLLPGKAVTSFVMGSRE
                     MYAWVHDNPGIEMRPSSFTNDPFQISRNDDMVAINSALAVDLTGQVAADSIGSRFFSG
                     IGGQVDFIRGAARSKGGRPIIALPSTAKDGTVSRITAVLAEGAGVVTSRGDIHYVVTE
                     YGVADLWGKNIRQRTMALIGIAHPDFRGELLATARQRRFIFPG"
     gene            complement(11978..12631)
                     /locus_tag="PLB01_04090"
     CDS             complement(11978..12631)
                     /locus_tag="PLB01_04090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HQQ76512.1"
                     /translation="MADMDSRAGLSYATREILDWLAGVHAPQDAALARAFDAPAAHGM
                     PAIQLGASEAKAVALLLRLAGARKVVEVGTLAGYSALAMARALPDDGHVWTIEFDPKH
                     VRVARENLAAAGLASKVTVLEGAGLDVLPTLEPFGPFDAVFVDADKRNYDGYGRWAAR
                     NVRKGGLLIGDNAFLFGRLLEDSAEAAAMRRFHEEARETFDTVCLPTLDGMLVGVRR"
     gene            complement(12651..13742)
                     /locus_tag="PLB01_04095"
     CDS             complement(12651..13742)
                     /locus_tag="PLB01_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQQ76513.1"
                     /translation="MPTSRRAFLEEVSFASLIAGFPLSAQAALTKYAEKPPEPVFPKA
                     LQPGMTVAIVTPSSPGGGLADTDWGVEIVKALGFKARVFPHVGESTRYLAGPDEHRAA
                     DINAAFADPSVDGIFCLHGGYGASRILPLLDYEAIRKNPKVLCGYSDITALLNAIHRL
                     TGLVTFHGPVGSEVQTDYTLASFRKVLMTAQPAGRVAEPPAPKGPPKEIVVDKENWIY
                     KLAPGKGRGRLVGGNISVFSTLVGTPFEPELKGRILFLEEVGEDPYRIDRWLTQFLLT
                     GKLSGLAGVALGKFSRCTPGDYKPSYNGFGQWTWQEVCKDRLGKLGIPVVANLVFGHV
                     PDKATLPLGVMAELDGDAGTLTLLEAAVR"
     gene            complement(13753..14592)
                     /locus_tag="PLB01_04100"
     CDS             complement(13753..14592)
                     /locus_tag="PLB01_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HQQ76514.1"
                     /translation="MTVFEILLPAFVASLILTGIHSYLGIHVVERGVIFVDLSLAQIA
                     AFGGTVGYLAGHEMHSTGSYVYSLVFTLLGAAIFAFTKSHGRTRIPQEAIIGIVYAVS
                     AACAVLVMSKATRETEHLKEMLVGNILSTPWRDLAKTAALYAVVGVFHYVFRERFLLI
                     SRNEAEAVRRGWNVKFWDFLFYASFGFVVTSSVEIAGVLLVFSFLIVPSVAAMLFAER
                     IGVRLAIGWTMGVVVSAVGVWISFTLDLPTGAAIVATFGAALLVLAAVRRFVPGPWRA
                     ASA"
     gene            complement(14589..15503)
                     /locus_tag="PLB01_04105"
     CDS             complement(14589..15503)
                     /locus_tag="PLB01_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HQQ76515.1"
                     /translation="MKTKLLSLALVLTLAPIAEAKRLKVVTTLSDFASLAATIGGDRV
                     QTDSLAKGYQDPHFVEPKPSFVLKLHDADVLIVAGLELEIGYLPPLLDQARNEKIRPT
                     GAGYLDASAGCEILDRPTGPVTRAMGDVHPYGNPHYWLDPKNGLVVARAIAAKLTALD
                     PAGAADYAKNLADFEVKLKAGEARWAARLAPFKGAKIVTYHNSWPNFAKAFGLDVVGF
                     LEPKPGIPPSPQHKLELVNLMNAQKVPIILMEVYFDRKDPDFIASKTGASVVMIPPSV
                     GGEPAAKDYISLFDVDVEKIAAALEKRK"
     gene            complement(15511..16608)
                     /locus_tag="PLB01_04110"
     CDS             complement(15511..16608)
                     /locus_tag="PLB01_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76516.1"
                     /translation="MKENPGRRAAVPLFLFAEFFLLSLSAASAAAQTAPAPSGNVFNP
                     NISVVGNALGFVGHNSVDNQPSLQLKESEIALQAIVDPYARADFFVSLDSEGGVGIEE
                     GFITFLTLPGDFIAKAGKFKAAFGKVNTEHPHVRQYADTPLVVLNLLGGEGGISDAGL
                     SVARLIPMPSEIFLEATAQVFRGTTENLFDAHSRNDLEPLFHLKAYKDLSESTNLELG
                     GSWTRGNGVEGPGLHTTLAGADVSFRWKPLRQGLYRSLLVRGEWMTSERETAAGTTVR
                     ADGWYALAQYQLGRRWNVGYRHDESDHADNATLRDRGDSAILTFAPSEFSTVRGQLRQ
                     VRYAQPAGPVRATELLVQLQFVIGAHGAHPF"
     gene            complement(16670..16999)
                     /locus_tag="PLB01_04115"
     CDS             complement(16670..16999)
                     /locus_tag="PLB01_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76517.1"
                     /translation="MRARLVRAAVLALLAPLALGGAFGHVDGIEALSHGTQLFAPDHP
                     NHAPFIPQPEHACAACVVANLLPPASAPVFLAPPASRLLPEALAERAASAPVLPGYSG
                     RSPPSAA"
     gene            complement(17034..19574)
                     /locus_tag="PLB01_04120"
     CDS             complement(17034..19574)
                     /locus_tag="PLB01_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQQ76518.1"
                     /translation="MVSVVLASVLAAAGWPNVALTRVTTGFASPTQVTSAGDGSGRLF
                     VVEQRGLVRIVKDGAVLPAPFLDIRDRVSCCGERGLLSIAFEPGRGPKTRFWANYTDL
                     AGNTQVSRFSFSGDAAFASTEVKILSVGQPFANHNGGQLAFGPDGYLYVGMGDGGSGG
                     DPLNNGQSLGTLLGKILRVDVLGGTVPYAIPPSNPFIGSSTARPEIWAYGLRNPWRFS
                     FDRKTGDLFIGDVGQGAWEEVDFQAAGFAGGANYGWRVTEGAHCYNPPSGCVTTGMTP
                     PVAEYSHANGCSITGGFVYRGRDFARLGGIYFYADYCSGRVWGLRRGATGWETAELLR
                     PGYAFTSFGESDDGELLAVDGSSGTLYRLVDADGPLAILTVPVVVDVSGASGARFVSE
                     LTLGNRGASDATVSVTFTASDALGSRGSGTFTTTVAAGRQLVFADALQWLRQNGLFIP
                     LGDQGGTLRLAYSGLVSGDAAFASVRTLAVLPEGRAGLAYPALAPNETLDAPVALYGL
                     REDASFRSNVGLLNAGDPATGGSVSLRVTVRSGAAGDGRSVTLPDVTLGPGQWTQLNR
                     VLAAVGYANGWVTVARVGGTDPFYAYGVVNDNVTGDGAFVAPVAAGRTATVLTLPVAV
                     HTDVFTTELCLTNAGDVPVRLSFPGSTTGEDAVLQPQEQRFVSDVLPYLGFSAQAMAL
                     RVSSKSGAAAFHASARTASRAAAGGTFGVAYSALAVGEVAFSETWLFGLKQDGLTRSN
                     LALANTNPPVTGSPPTPLPVELTLEFFNGETGLPAGSRTISVDSFSPTWIQVNAPLSP
                     LGIRSGYARVRAPAGNSLQFFAYGIVNDGAVPGAGTGDGSYLGMAGLK"
     gene            complement(19614..19952)
                     /locus_tag="PLB01_04125"
     CDS             complement(19614..19952)
                     /locus_tag="PLB01_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQQ76519.1"
                     /translation="MHVEVRKVGDVVIVDLKGRLLAGVGDEILRNVVNELLAEGWKKI
                     LLNLSGVTAIDSSGTGELVASLKICGKFDANLKLLNLHERVRKSLHLSELLPVFEVYD
                     DETAALRHFA"
     gene            20000..21304
                     /gene="lysA"
                     /locus_tag="PLB01_04130"
     CDS             20000..21304
                     /gene="lysA"
                     /locus_tag="PLB01_04130"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQQ76520.1"
                     /translation="MPATTLHEGLEALRADRASEPAALSAVSRLASEFGTPLYVYDKA
                     AITGRFRALKAAFAPHFPKLRLHYALKANGSLAIGRILRAEGAFPEVVSLGEILTALR
                     AGWSGADVLFTSSSKTPEEIAKTIELGAVLNADSRDELEQASAAAVKAGRTVRLSFRL
                     NPGVDPDTLHHINTGIPESKFGVHLDGGDALAAYARAKELPGVAIAGIHCHIGSQIAD
                     TAGYEKTALKMLAFAKELKDRLGIVLEFIDLGGGFGVPYRDGESVMSPETLAAALEPI
                     WKEGVAAAGYEPALWLEPGRSLVAPSGFLVTRVNTVKTTPLKTFVNVDAGSNTLLRPA
                     LYGAYHRARVVAAAPGAPRLLDVAGDVCETGDILAEERTLPLPKAGDHVVFLDAGAYG
                     FAMASVYNARPLPAEVLVDGDAATLIRRRGTYDGLFRDEVSD"
     gene            21316..23130
                     /locus_tag="PLB01_04135"
     CDS             21316..23130
                     /locus_tag="PLB01_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQQ76521.1"
                     /translation="MRRISPTAAVLLASLLAGCGANPFPAQTVDFGQLQPEPPRALSM
                     PLADKVGQLFVVQANGVFMSEDSEPFQTLRHHVVDNRIGGVMLSRSSVYGAAVLVQKL
                     QEMARTPLLVSADLEAGSGMRFEDTTYGPWAMAIAATGDPSLAERRGRATAEEARAIG
                     VAQVYAPVADVNVNPDNPVINVRSFGEDPSDVARYVVATVHGLQAGQVLATLKHFPGH
                     GDAATDSHRSLVTIPGDRARLESVELVPFRAGIKAGAESVMIAHVSVPSWDATPAPIL
                     AHPVKPADVIGETFEIEKGPVPATVSAPIVTGILRKELGFTGLVVTDAMRMGGITSHY
                     EPGEAAILAILAGSDQILLSSDTDAAIKAVMAAVKSGRISEARIDESVKRILDAKKRL
                     NLYDKGSPFVGKISKIVGTQAHVDLEAEIARRSMTLVREKAGALPFRRDAKILSLVVA
                     DEPTLYGPAGTLDKELKTRVPGLKSVRLDTRSTPEEARAAADAAKDADAVLLSLFVRA
                     RSGQGRFVIPDAARSVIPALLASGKPVVAVAFGSPYLLRDFPDLPTYLCAWGSQDVVQ
                     VAAAKALFGEATIDGRLPITIPGLAKRGDGISKPVLPR"
     gene            23248..25026
                     /locus_tag="PLB01_04140"
     CDS             23248..25026
                     /locus_tag="PLB01_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ76522.1"
                     /translation="MNALCSLSRAALFSLASAAMASDVDDARALFERNLAAIQKRDKA
                     AYLACYLDGKTLVRTSPEGFELGYEDFAKQAGEKWPDTLAADDLRLTPVSPGVVYGTY
                     RYRVRYGAEEHAGLSERLFLRTPRGWRIAVTSAWEAPAGTPPPPRAFTGATLVDGTGS
                     PAVANAVVLVRNGRIDCAGPASACPVPEGVAVTDAKGSWIVPGLVDAHVHFAQTGWAD
                     GRPDAFDVRAKHPYDAVQKELREHPERFFRSYVCSGVTSVFDVGGYAWSLDLAARAEN
                     DSLAPRVRAAGPLLSTIDFWLNLSGERQFMFLKDAESAKTGVAYLASRGASAVKVWYV
                     VTPDRPVEASEAAVLAAGAEAKKAGLPLIVHATGLAEAKIALRAGAKLLVHGVTDRPV
                     DDEFLALAKQAGATYNPTLVVLDGYVAVAKAAAAGAPSPFDDPNGCADPASRARLAAT
                     PEIDGASIAAKVRDRETRTAERKAVGLANLRRVRDAGIPIAMGTDAGNPGTLHGPSVH
                     AEMEAMQSAGMTPMEVLVASTRGGARAMGRDDVGTLEKGKRADFLLIGADPLLDAANL
                     RKLNAVVRGGVYRTQAELRAIVASEP"
     gene            complement(24998..>25360)
                     /locus_tag="PLB01_04145"
     CDS             complement(24998..>25360)
                     /locus_tag="PLB01_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76523.1"
                     /translation="PDAPAPRPGAVLVSAAVASGSALALVVGLAPFTSTAPGLALART
                     VVLCGATLVVAWTVRFVRFGVVSLLAYPLLAAAGVKLLIEDLRVGGPATLTAAFVFYG
                     VTLLLAPRLLRARSRRSP"
BASE COUNT         3709 a         9010 c         8797 g         3844 t
ORIGIN      
        1 tcgccgcttt tcttcttaga aggcgcgggg ggtgcaggcg cggtgggcga ggacgtcgcc
       61 gcgccgggtg cgccgccgtc acccccgtcc ttcttgccct tcgaagaatc cgaaccgccg
      121 cctcccgccg cctcgccgcc gcccggagcg ctcgaagttt tcccctcttc acctttcgaa
      181 gaactcttct ctttgtcttt gtctttctct ttcttctcct cggacttctc tccggcccga
      241 tccccggccg agcccgactt cccgtagtcg gtcaggtaga agcccgagcc cttgaactgg
      301 aacgcgggag acgagaggag cttcgtgagc gcgccgccgc actcgggaca cacgacgaga
      361 ggcgggtcgg agaagctccg gatcacctcg atcctctcgc cgcacttcgc gcactcgtac
      421 tcgtacaggg gcatcttcgg tcgtcctttc gtccgcggag cccgctcagc cgtcgccgtc
      481 gccgtcgccg ccggccgtgg ggattccgcc cggcgtcttc cactggatgc ggcggatgcc
      541 gctggtctgc gactcgcgcc gcgtgatgag gcggagcacc cagaacgcgt agaggagctt
      601 cgcgttcgcg ccgaggtctc cggggcccat cgtgatgagg tcgcgcagcg tgcgccggcc
      661 gtccacctgc gaaaggagcg tgacctcggc ggccgtgaag gaaacgtcgc gcgcctccgg
      721 cggggcctcc gccctctcga agatcgtcca cgaggggccg agccgcgcga cgcaccgccg
      781 cggatcgggc atccggcgga tgccgtcgac gatcgcctgt gacgtcggca cgtcgagccg
      841 gatgagctcg tcggtcttgt agcggccgac ctcgaacgtg accgacccct tctcccacga
      901 gaacgccgcg tagaggagtg ccttcacctg ctcggtgacg atctggcgga gctgtgcgtc
      961 cgagaggatg cccatctcga ccaggagctc gccgtggcgc cggccctgcc cttcggcgag
     1021 gtcgagcttg ccgacggagg ccttgagctc ccccgacgag atcagcgcgc tgcggcgcag
     1081 gtactcgccg agcgactcgg atcggtccgt cgacgtcgcg aagatgatgt tcccgccgag
     1141 gagatacacc cgcttcgtga cgttgcccga ctcgaccgtg agcacgcccg gcactcgata
     1201 gaagtggatc gtacggagga cctcgggcag cggctcctgc gcgagatcgc ccgtgtactg
     1261 gaaccggtct tccacggcgg acggattcta ctcacgacgc ggcggcatcc gcttgttagc
     1321 atgcctgcgg tgatcgcgtt cgaagaagcg ctcgcgctcg tgctcgaggc cgcagccccg
     1381 ctcccggcct gcgaggtccc cctgccggag gccgtctcgc gcgtcctcgc ggaggacgtc
     1441 cgcgcggatc tccccgtccc cggcttcgac acgacggcga tggacggctg ggccgtccgc
     1501 gccgccgagg cggttcgcgc gccgactctc ctcgcggtgg ccggcgcctc gggggccggt
     1561 tccgtgccgg gcaaacttcc gcccggctcg gcgtggaagg tcatgaccgg cgcgccgatg
     1621 cctccgggaa gcgacgccgt cgtccccgtc gaggatgccg ccgacgtcga ggggggctcc
     1681 gtgaggctcg acgccgttcc gaagagcggc gcgcacatcc gccgccgcgg agaggtctac
     1741 gaggccggcg cgctgctcct cgcggcgggc cggcgcctca cgcccgcaga cctcgtcctc
     1801 gccgccgccg caggacgcga gaagcttcgg gtcgcccgcg cgccgcgcgc cggggtcctc
     1861 gtgaccggtg acgagctcgt gccgccgggg acgcagcccg gaccgggcga gctgcgcaac
     1921 acgaacggcc cgctcctcct cgcggccctg cgccgcgcgg gagcggacgt cgtctccctc
     1981 ggcgtcgcgc cggacgacga ggcggctctt gcccgcgccc tgcgtgacgc gctcatcgcg
     2041 aagttcgacg tcctcctcac gaccggcgga gtctcggcag gagactacga cttcgtgggg
     2101 aaggcccttg ccgccgaggg cgcgcgcgtg cgcttccaca aggtctcgat acggcccgcg
     2161 aagcccgtcc tcttcgccac gaagggtccg acgctcgtct tcgggctgcc cggcaacccg
     2221 gtctcggcgg cggtcgcgtt cgacttcttc gtccgtcccg ccctgcgcgc ggcggccggg
     2281 ctcctgcccc cgcttccgcc ggctgttccc gcgcggctca cggcccgcgt gaagaacaag
     2341 gggccgcgcc tggccttcca tcccgccctc ctcgcgtggg atgcgggccg cctgtgcgcc
     2401 gagcctcttg cgacgcgagg ctcccacgac atcctcgcgc acgcgcaggc ggacgcattc
     2461 ctcgagctcg cgcccgagtc gtcgtgggag gcgggcgatt ccgtccccgt ccaccgaggg
     2521 acggccgaga gcaccttcta gcggcgcttc gctttcttcg gggaggcctt tttcgccgcg
     2581 gccttcctct tcgccccgcg gcccgctttc gggaagaggc ccgtgcggat cgtctcgacc
     2641 tgcgcgaggt gaatgcggtc gtgccctgcg tacatcgtca cgaggcggcc gagcgactcc
     2701 tcgccacgct cggcgtgaat gcccacgcgg ttccacgcgc tctcgcccac gcgctcgagg
     2761 aggccgatgt tcacggcccg cgccatcgcg aagtcgtcga ggagatcccc ggttgccgcc
     2821 tttccgacgc cgaggttcgc gacgaacgcg tcctggtcga agccgggcag cggagggcgg
     2881 tccatcgcgg cgatgaagcg gtagcggccg ccgaggacga cctcgttgtc ggcgaggtgc
     2941 gcgacgatct ccttgatgct ccacttgccc ggcgcgggcc tcgtcgcgag ctgcttctcg
     3001 gtgaggccgc ggacgaggcg cctcaggaga tcgggcgcct cggccatcgc cgcgacgggg
     3061 tcgtcgccgc cgagggcttc gaggtagcgg tcgggaacga cgggaccggg agatccagcg
     3121 gccatgtgag ctcctccccg cgtcagcgga tggcggaggc cacgtcgccc gcggaaaccg
     3181 gttccgaact ccgcagccgg atcttgccgc cggtgcgcca ggcgatccag tagggcgcct
     3241 tctcgatgcc cgagtcgtgg ccccagtcgc cgcccgggtc gaatacgacg ggccgccgga
     3301 tgccccgggc gcgcttgacg gcgtccgcga tcggctcgcc ggccaggacg atgtaacact
     3361 cgataccctc ttcggcgagc cgggccgaca gcgcgatcgc gccctggagc gcatcctggg
     3421 acttcctgcc gacggtcggg gtgaggtaca ccgccgcgca gcccggcggc gggcagttcc
     3481 tcatcacgac gctctccatg ttcgcctcga agcccatgta cctctgaagg ctctgctcgc
     3541 cgacggtgga gggggccttg aagtagagcc aggccccgac ggcgaccgcg aggatcgtca
     3601 ggacgatcgc cgtaccggac gggcccatgg acacgggctt gtcctcgccg aagcgaaaac
     3661 gcgacgtcgt gttcggcccc gggcccgcga ggggaggcga cccgcctgcc ggcgtcgctc
     3721 cggacggggc gcgggagccc ggctgccgcg gattggacgg tgaggagact gtgcccatga
     3781 acgggctaat tctacgcaat cccgtcgcgg gacggaatcc tcggacagag aactgcgggc
     3841 cggcccaatc ccgggccggt caggccgcgg ggcctttctt gagcgtcacg acgcccgacg
     3901 tcgaccggat cacgagcttc ggaccgcctc cgttcagacg gccttcgccc agaatgtggc
     3961 cggaacgccg cgcaaccgag atttcgggga actgggaggc gatggagtcg gcatccaccc
     4021 cgcctcccga cacgacgatt tccacgtcgg ccttgacgtt cgcgggcagc gtcaccgtca
     4081 cgtcgccgcc gctcgtcgcg agctcgcccg cggcgccgcc cgtactcgtg atctcgcacg
     4141 tgatcgagcc gccggaggtg cgcgccgtga cgggcccgcg caccttcttc aggacgacgt
     4201 cgccgccgcc cgtccgcgcg gtcacggcgc ctcccgccga gcggacgacg acgtcgccgc
     4261 cactcgtctc gagccgcacg tcgccgctca ccgagcccgc ccggatgtcg ccgccgaacg
     4321 tcgtcgcctt gaggtcgccc gtcacggctt cgacccggat gtcgccgccg gccgtcgaga
     4381 cctcggcgcc ctttccggcc tcgcggacgc ggatctggcc ggaccggctc gcgatcgtga
     4441 cgcggcccga gaccttgccc acggtgacgt cccccgaggc gatcgccggc gtgtagtcgc
     4501 ccgcgacgtc gcccacggtg atcgaacggc cccaaggatc cggcgggatc ggcgccaccg
     4561 gtgcgacggg agcgactccg gcggccggag gcgccggcgg ggccggcacg gctcgcgggg
     4621 cgggcgccgg ctccggcatc gggtgctcgc gcacgacgat ccgcggcagg ctcggcatcg
     4681 gcggaacggt gacgacgtgc gtggagcgcg gggacgtcag gcgcttggcc tgggcttccg
     4741 tcgtgccctc ggcgaggatg aggatcttcc cgttcaggtt cgagacgcgc accgtggcgc
     4801 cgccgtcccc gaccgcgccc tcgtaggccc ggttcaggtt caccgtgatg ctgcgcgaga
     4861 tctcctggct gagaccggcc atctcgcggt tgagctcggc catcgcctcg ttcagctcgg
     4921 agaggtcgat gacgatgtcc tcctcgccgg agtccttcct gaccttggcc tggtccttct
     4981 ccttcttccg gatctcggcg cggagcttgc gctgctcggc gcggatcctt tcgcgctccg
     5041 cgcgcatctc gtcgttctcg cggacggcct cgggcgccgg cgtcgggagg gagaagctcg
     5101 agaggatctc gccgctcatc gtgtggccct cgagccggaa cccggcctgg gccggcagcg
     5161 tcatcgagat cgaaccgttc accgcccgga cgtcgaggac ggaccccttc gggagttccg
     5221 cgaagcccgc gacgatcgcg ccgttcaccg tgttgagctt cgcgtcgcgc cggccgccct
     5281 cgagcacgat cctgccgttc acggtcgtga gctcgaccgg ggccgcgacg cccgtcacgg
     5341 acacgccgcc gttcacggcc gagacctgca cggacatcga ggccggcacg gtgatgcggt
     5401 acttcgtctc gctccgccac tggtcgtcgc tccggcttcg aacgttccac ccgcggcccg
     5461 agtgccggac ggtgacgccc ggctcctcgg tgtagaggga gaggttcccg ttctcgttct
     5521 cgaagcggac cttgacgtcg ccgaggttct tcttcgcgtc gccgtccgtc gcgccccagg
     5581 ccgtcacgtc gacgtcggcc cggaaggcgg ttccggccac gatctccacc ttgccgttga
     5641 ccgtctcgac ggcgagggat ttcaggttgc ccgaggcgtt gaacgtctcg gtccgtgtgg
     5701 cctttgcgtt ccactccttg gcttcggcct tcagtccggc cgcgagcgag aggaggacga
     5761 gggcgacgat cgcgagcgcc cagacgaggg cgcgaccctc gcgtcgcggg ctttcgtggg
     5821 tggcgaagtt cgtgctcatg tgaaagagac gctccaatgc cgaaaaggtg tcgtcagatc
     5881 tcgatcttcg agagcgcgag cgcggcctgg ctgcggacgt agccgttctc gcggtcgtcc
     5941 gcggccttct gcctgaggct ctcgatggag gacgcgtcct tgaggacggc cgcggccttc
     6001 gcgagccctt cgacggcgag aatccggacg gcgggattca cgtcgttctt gagcgcggcg
     6061 gcgagcgcgt cgcgggtttc gggcgtcggc ggcatctgcg cgagcgcctc ggcggccttc
     6121 ttgcggacgc ccgggttctt gtccgtctcg agcgtgcggc gaagcagggt cacgatctcg
     6181 ggcgacacgg ctcctccgcc gcgcgtcccc tcggacacga ggtcgagcgc cttccccttc
     6241 gcgccctcgg atccggagct cccggacatg agcgacacga ggagattcgc gatcgcctgg
     6301 tcttccggct tgccgacgac cgtgtagcgc gtcgtcacgt cgaaggacac ggcgacgcgt
     6361 cccgccgcgt ccgcgggcgc gtacgcgacg ttcgcgaggc gaggcttgcc cgacaggtcg
     6421 agcgcggaac gggaagcatc cagcgtccgg ttggacacga gggagacgtt ctcgccgccc
     6481 cggaacggcg cgggggtggg ctggaacgcg gtcgagcccg gaagggagcc gccgccggat
     6541 gcacgtgcga ggaagaagcc ggcgacgccc gccacgagga cggccgccac cttcaggaga
     6601 ggggactcgg cgccgcgcgg cgcgaaggga cgcacgttcg tcgctcggcg ggtctcggcg
     6661 gccagggcgg ccgtcgtccg cgcctcgaag gcggggctcg cgacgaggtc ggaagcgtcg
     6721 tcgtcgccga gacgggccca cacggattcc atgtcggcgg cgatcgccga gcacgcggcg
     6781 cagccggcga cgtgcgccgc cagcgccgcg gcgccgtccc cggcgctctc tcccgcgagg
     6841 cgggcgagga cgaattcacg gaccttcagt gactcgcggc attccatggg gtctctcctt
     6901 cggagaacag ggtcttgagc gtctccattg cccggaagat ccgggtcttg acggccccct
     6961 cgctgatcga gagggagcgg gcgatttcgg cgtacgggag cccgtggtaa cgggccagga
     7021 cgaggacctc gcgctgctct tcggacaggc gggaaagcgc gcggcgcacg gcggcgcgcc
     7081 tctgcctctc gacgaggcgc aggtccacgg cgatctcggg ctgcgccgtt tcggcgaccg
     7141 cctcgagcgg gaccgtcggc cggcgggtgc tccggcgcag gcggtcgcgg caggcgttcg
     7201 cggcgatcgc gaagagccac gaggccactg aggcccggcc gtcatactgg ttcgcacgcg
     7261 cgatgacctt gaggaagacg tcctgtgtga cctcttcggc gtcggccgcg tttccgagca
     7321 tccgcccggt gaacttcagg acggggccgg accagcgctg gaagaggcgc gtggccccgg
     7381 cttcgtctcc gcgccgaacc tgctcgagcg cctcgagatc cgcttcctga tcggggctga
     7441 tcccgtccgg ctccacgcac ccctcccgct ctctggcgac ggcttccacg cgagtatgac
     7501 gggggacggg acgaaaggtt acggggaccc ggggccggtt tcagcccttc cggagcgcca
     7561 ggcttccctt gtccgtcctg aggctcacgc gcggcccgcc gccgttgacg ggtccgcggg
     7621 cgacgagacc ccgcctgtgg ctcccgcggc cggtctcggc ggggaactcc acgttcaggc
     7681 tgcctcggcc cgactcggcc gacagctcga agcccgccgt gccgggcagg tgcaggacga
     7741 tcgagccctt gtacgtctcg gcgttcacgt cgcccgagag tcggacgatc ccgacgttca
     7801 cctcgccctt gtatgtctcg accctcaatc cgccggagag cccgacgacg tccaggtgcc
     7861 ccttgtacgt gttcacgtcg aggtcggccg ccagctcctt cacgacgagg tcggacttgt
     7921 agtccttgat ccgaagaggt gcgctcttcg gcatatggat cgtgtagcgc acgaacgggc
     7981 gcgccgtgca tctgccgaac cagctcgtcg cctcctcgag tgcgtcgtag tccgacgtga
     8041 tcgacacgcc gccgtggcgc ttctcgatca cgacccgggt ctgatccacc cagcgggcca
     8101 tgtccttggg cgagccgcag ttgtcgtccg cctcgatccg ggcctcgacg gcgacttcgg
     8161 acctgtccca cgtcgtgacc ttgacggttc ccttgtaggt ttcgacccgg acgctctccc
     8221 ccgcctcgag cggcagggtc ttggcgacgt cccgggacgg gagcacggcg gcggcgggaa
     8281 gggacgcggc gaggagggcg agggtagggg cggtcaggcg catggagggc tccttgtcca
     8341 cttagacgaa atccgtcgcc ggaagttctg cgtaagatgt ggaagtgggg agtagctacc
     8401 cgccccacgg cgggtcggaa gccgtcaaca cggggattcc cgcggggtcc cccggtttcc
     8461 gaaggcgagc cgcgatgccc gcggcctcgc cccggcgaga ccaccacggc cgtcgtccgt
     8521 ggagcgtctc ggaggtctga tgcattccct gcccgtctgg atcgccttca acgtattcgt
     8581 cctcgcgatg ctcgcgctgg acctgggcgt ctttcaccgg aaggcccact ccgtctccct
     8641 caaggaggcg ggaatctgga gcgccgtgtg gatcgcgctc gcccttgcct tcggggcggg
     8701 actctggcac ttcgagggca aggatcccgc cctccagtac ctcacgggct acctgatcga
     8761 gaagtccctc agcgtcgaca acatcttcgt gatcgcgctg attttcacgt acttcgcggt
     8821 gcccgccgcc taccagcacc gggtcctgtt ctgggggatc ctcggcgccc tcctcatgcg
     8881 cggcgccctc atcgccgcgg gcgcgtttct cctctcgaaa ttccacttcc tcgtgtggat
     8941 cttcggcgcg ttcctcatcg tgacgggcat caagatggcc ctccacgaca acgcggagct
     9001 ccaccccgag aagaacccgc tcgtgcgcct cgcgcggaag atcttccggg tcacgaacga
     9061 ctacgagggc cagaacttct tcgtccgccg ggacggagcc ctctgggcaa cgcccctatt
     9121 cctcgtcctc ctcgtcgtcg agagcatgga tctcgtcttc gcggtggact ccgtccccgc
     9181 gatcttcgcg gtcacgtcgg acccgttcct cgtctacacc tcgaacgtct tcgcgatcct
     9241 cggcctgagg tcgctctact tcctgctcgc cgggatcatg ggacgcttcg tgtacctgaa
     9301 gctcggcctc tccgtgatcc tcgtcttcgt gggcctcaag atggcgttcg tcgagctcgt
     9361 caaggttccc gtggggatct ccctcggagt catcggcctc gtcctggccg tctccgtcgt
     9421 cgcgtccctc gtcaagacga gccccgcttc ccggaagggg gcgtcctcgt gacctccgcg
     9481 acacgcatcg cgaccgtcgc cggcatcgag atcaagatcc acgtcacgtt cgggatcatc
     9541 ctcgtgatcg gagccgtcca gtgggggatc ccccatggcg ccgccggggc ggccttcggc
     9601 gcggccctca tggccgcgct cttcttctgc gtcgtcctcc acgagctcgg gcacagcctc
     9661 gtcgcccggg gcttcggcct gcccgtcaag gagatcgtcc tcctcccgat cggcggcgtg
     9721 gcacggatgg agaagaaccc ggagaagccc gtccacgagc ttctgatctc ggtcgcgggc
     9781 cctctcgtga acgtcgcgat cgcgggcgtg cttctcgccg ccgtcggcgc gaggttcggg
     9841 ccgctcgacg ccgccggcct cgcggcgcgg ctgaagggcg cgccgtccgt cgagacggcg
     9901 ctcctctggc ttctctccgt gaacgtgatg ctcttcgtct tcaacatgat cccggcgttc
     9961 ccgatggacg gcggccgcgt gctgcgcgcg ctcctctgga tggtgatggg tttcgggccg
    10021 gcgacgcgca tcgcaaccct cgtcggccag gtcgccgcgg tgggcctggg cctctggggg
    10081 ctcctctcgg gcaacctctt cctcgccctc atcgccgtct tcgtcttcct cggggccggc
    10141 caggagcgcg ccgacgagca ggcgcgcgtc gtgctcggca cgctgcgcgt gggcgacgcg
    10201 tacaacaagc acgcgatcgc gctcgcgccc ggcgacacgg tcgggagcgt cgcggactac
    10261 atcctcacga gctaccagcc cgacttcgcc gtcctgcagg gaggggccct cctcggcgtc
    10321 gtcacgcgcg cggacgttct gcaatccctt gcgacggaga cccacgactc gtacgtcacg
    10381 ggcgtcatga agcgtgacgt cttgcgcgtc gaagcggacg cgccgctcga ctccgtccgg
    10441 cggctcctcg tggagaaggg cgagcgggtc gcagccgtga cccggggggg cacgtatctc
    10501 ggcctcgtga gcctcgagga catcgccgag gcgcttctcg tcgttgcgtt ccgggccgcc
    10561 caggacaccc ggcggaaagc cgtcgagacg gcctgagcct cagcccggga agatgaagcg
    10621 ccgctggcgc gcggtggcga ggagctcgcc ccggaagtcc gggtgcgcga tcccgatgag
    10681 cgccatcgtg cgctggcgga tgttcttgcc ccagaggtcg gcgacgccgt actccgtgac
    10741 gacgtagtgg atgtcgcccc ggctcgtgac gacgcccgca ccctctgcga ggacggcggt
    10801 gatccggctg accgtgccgt ccttggccgt cgaggggagc gcgatgatcg ggcgcccgcc
    10861 cttgctgcgc gccgcgccgc ggatgaagtc cacctgaccg ccgatgcccg agaagaatcg
    10921 cgagccgatc gagtccgccg cgacctggcc cgtgaggtcc acggcgagcg ccgagttgat
    10981 cgcgaccatg tcgtcgttgc gcgagatctg gaacgggtcg ttcgtgaagc tcgaggggcg
    11041 catctcgatg ccggggttgt cgtgcaccca cgcgtacatc tcgcgcgagc ccatgacgaa
    11101 ggacgtcacg gccttgccgg ggaggagctt cttgcgccgc cccgtcacga cgccggactg
    11161 gatgaggcgc atcgcgccgt ccgacatcat ctcggtgtgg atcccgaggt cgttgcggtc
    11221 ggcgagcgcg ctgatgacgg cgtccgggat gcggccgatc cccatctgga ggcacgcgcc
    11281 gtccggaatg aggctcgccg cgtggagacc gatctggcgg cagacgtcgt ccggggcctc
    11341 ggaggcgagc tcgaggagcg gctcgtcgac cgggatgagg tgcgcgatgc ggtccacgtg
    11401 gaggaacgag tcgccgagcg tccggggcat ctgcgggttc acctcggcga ggacgagcgg
    11461 cgccgtgtcc acggccgcgc gcacgatgtc gaccgagacg ccgaggctga cgtagccgtg
    11521 ccggtccggc gggctcacct ggatgagcgc gacgtcgatg ccgacgcggc cggtcgagat
    11581 cagggtcggg atctcggaga ggaagaccgg caggaagtct gcccggccct cctgcacggc
    11641 gccgcggacg ttggcgccga tgaagaacgc catgtggcgg aagcgctgct cgagccccgg
    11701 cgccacgtac ggcgccttgc cgagcgtcat gaggtgcacg atctcgttgt tgcggaggtg
    11761 ggtgccgtgc tcgacgagtc cctcgacgag cttttgcggc tcgcccgcgc ccgagccgat
    11821 gagaatgcgg cgccccggcg ggatcgtccg gatggcgtct tcgatcgacg aaatcttcca
    11881 ggagaagcgt tctttccagt cattcatacg agatgaatgt aacgctcgcg tacaaggacg
    11941 ggtaccgatg cgtttgaatc ccccgggacc ctaagactca acgcctcacg ccgacgagca
    12001 ttccgtcgag ggtcggaagg cacaccgtat caaaggtttc ccgggcttcc tcgtggaagc
    12061 gcctcatcgc ggcggcctct gcggagtcct cgagaaggcg cccgaacagg aacgcgttgt
    12121 cgccgatcag gaggccgcct ttgcggacgt tcctcgcggc ccagcgcccg tagccgtcgt
    12181 agttccgctt gtcggcgtcg acgaagacgg cgtcgaacgg gccgaacggc tcgagcgtgg
    12241 ggagaacgtc gaggcccgcg ccctccagga ccgtgacctt cgacgcgagg ccggccgcgg
    12301 cgaggttctc gcgcgcgacg cgcacgtgct tcgggtcgaa ctcgatcgtc cacacgtggc
    12361 cgtcgtccgg cagcgcgcgc gccatcgcga gagccgagta tcccgcgagc gtcccgacct
    12421 cgacgacctt ccttgcgccc gcgagccgca ggaggagggc gacggccttc gcctccgacg
    12481 cgccgagctg gatcgcgggc atcccgtgcg cggccggcgc gtcgaacgcg cgcgcgagcg
    12541 ccgcatcctg gggcgcgtga accccagcga gccagtcgag gatctcgcgc gtcgcgtacg
    12601 agaggccggc gcgcgaatcc atgtccgcca tcaggtcccc cgcgcgccga tcaccggacc
    12661 gccgcctcca gcagcgtcag cgtgcccgcg tcgccgtcca gctcagccat gacgccgagc
    12721 gggagcgtcg ccttgtcggg cacgtgcccg aagacgaggt tcgcgacgac ggggatgccg
    12781 agcttgccga gccggtcctt gcagacctcc tgccacgtcc actggccgaa gccgttgtag
    12841 ctcggcttgt agtcgccggg cgtgcagcgc gagaactttc cgagcgcgac gccggcgagg
    12901 ccagagagct tgcccgtgag gaggaactgc gtgagccagc ggtcgatgcg gtacgggtcc
    12961 tcgcccactt cttcgagaaa gaggatgcgg cccttgagct cgggctcgaa cggcgtaccc
    13021 acgagcgtcg agaagacgct gatgttcccg cccacgagcc ggcccctccc ttttcccgga
    13081 gccagcttgt agatccagtt ctccttgtcg acgacgatct ccttcggcgg ccccttcggg
    13141 gcgggaggct ccgcgacgcg gcccgcgggc tgcgccgtca tgagcacctt gcggaacgac
    13201 gcgagcgtgt agtccgtctg cacctccgag ccgacggggc cgtggaacgt gacgaggccc
    13261 gtgaggcggt ggatcgcgtt caggagcgcc gtgatgtcgc tgtagccgca gaggaccttc
    13321 gggttcttcc ggatcgcctc gtagtcgagg agcgggagga tgcgcgacgc gccgtacccc
    13381 ccgtgaaggc agaagatccc gtcgacggac ggatccgcga acgcggcatt gatgtcggcc
    13441 gcgcggtgct cgtcgggccc cgcgaggtaa cgggtggact cgcccacgtg cgggaacacc
    13501 ctcgccttga acccgagcgc cttgacgatc tcgacgcccc agtccgtgtc cgcgaggccc
    13561 ccgccgggtg acgacggcgt gacgatcgcg accgtcatcc cgggctggag cgccttcggg
    13621 aagacgggct cgggcggctt ctccgcgtac ttcgtgagag ccgcctgcgc ggagagcggg
    13681 aacccggcga tgagagatgc gaacgagact tcttcgagga acgcgcgtct gcttgtcggc
    13741 atgcgcggga ttctaggccg aggcggccct ccagggtccc gggacgaagc ggcggacggc
    13801 cgcgagaacg aggagcgccg cgccgaacgt ggcgacgatg gctgcgccgg tcggcagatc
    13861 gagcgtgaac gagatccaga ccccgactgc ggacacgacg acgcccatcg tccacccgat
    13921 cgcgaggcgc accccgatgc gctcggcgaa gagcatcgcg gcgaccgacg gcacgatcag
    13981 gaacgagaag acgaggagca cgcccgcgat ctcgacggag ctcgtgacga cgaaaccgaa
    14041 cgacgcgtag aagaggaagt cccagaactt gacgttccag ccgcggcgga cggcctcggc
    14101 ctcgttgcgc gagatcagga ggaaccgctc gcggaacacg tagtgaaaca ccccgacgac
    14161 cgcgtacagc gcagccgtct tcgccaggtc gcgccacggc gtcgacagga tgttcccgac
    14221 gagcatttcc ttgaggtgct cggtctctcg tgtcgccttc gacatgacga ggaccgcgca
    14281 cgccgccgaa accgcgtaga cgattccgat gatcgcttcc tgcgggatgc gcgttctgcc
    14341 gtgcgatttc gtgaacgcga agatcgccgc cccgaggagc gtgaagacga gcgaatagac
    14401 gtacgagccc gtcgagtgca tttcgtgccc cgcaaggtat ccgaccgtgc ccccgaacgc
    14461 cgcgatctgg gcgagcgaaa ggtccacgaa gatgacccct cgctccacga cgtggatccc
    14521 gaggtaggag tggatccccg tgaggatcag actcgcgacg aacgcgggca gcaggatctc
    14581 gaagacggtc acttcctctt ctccagggcc gcagcgatct tctcgacgtc gacgtcgaag
    14641 agcgagatgt agtccttcgc ggcgggctct ccgccgacgg acggcggaat catgacgacg
    14701 gaagcacccg tcttcgaggc gatgaagtcc gggtccttgc ggtcgaagta gacctccatg
    14761 aggatgatcg gaaccttctg cgcgttcatg aggttgacga gctcgagctt gtgctgcggc
    14821 gacggcggga tgccgggctt cggctcgaga aagccgacga cgtcgagacc gaacgccttc
    14881 gcgaagttcg gccacgagtt gtggtacgtg acgatcttcg cgcccttgaa cggcgcgagc
    14941 cgcgcggccc atcgggcctc gccggccttg agcttcacct cgaagtccgc gaggttcttc
    15001 gcgtagtcgg ccgcgccggc cgggtcgagc gccgtcagct tcgccgcgat cgcgcgcgcg
    15061 accacgaggc cgttcttcgg gtcgagccag tagtgcgggt ttccgtacgg atggacgtcg
    15121 cccatggcgc gcgtcaccgg acccgtgggc ctgtcgagga tttcgcaccc ggcggacgcg
    15181 tcgagatagc ccgcgcccgt cggccggatc ttctcgttgc gcgcctggtc gaggagcggc
    15241 ggcaggtaac cgatctcgag ctcgaggccc gcgacgatca ggacgtccgc gtcgtggagc
    15301 ttcaggacga acgagggttt cggctccacg aaatgcggat cctggtagcc cttcgcaagg
    15361 gagtcggtct gcacgcggtc gccgccgatc gtcgcggcga gggacgcgaa gtccgacagc
    15421 gtcgtgacga ccttcagcct cttcgcctcg gcaatcggcg caagcgtgag aacgagggcc
    15481 agagagagaa gtttcgtttt catggtctcc tcagaagggg tgcgcgccgt gcgcgccgat
    15541 gacgaactgg agctgcacga ggagctccgt cgccctgacg gggccggccg gctgtgcgta
    15601 gcggacctgc cggagctggc cgcggaccgt cgagaactcc gacggcgcga acgtgaggat
    15661 cgccgagtcg ccgcggtcgc ggagcgtcgc gttgtccgcg tggtccgact cgtcgtgccg
    15721 gtaaccgacg ttccagcgcc gcccgagctg atactgggcg agggcgtacc agccgtccgc
    15781 ccgcaccgtg gtcccggccg ccgtctcgcg ctcggaggtc atccactccc cgcgcacgag
    15841 cagcgaccgg tagagtccct gtcgaagcgg cttccagcgg aacgacacgt cggctcccgc
    15901 cagcgtcgtg tggaggcccg gcccttcgac gccgtttccg cgcgtccacg agccgccgag
    15961 ctcgaggttc gtggactccg agaggtcctt gtaggccttg aggtggaaga gcggttcgag
    16021 atcgttacgc gagtgcgcgt cgaagaggtt ctccgtcgtg ccccggaaga cctgcgcggt
    16081 ggcctcgagg aagatctcgg agggcatcgg gatcagcctc gcgacggaga gaccggcgtc
    16141 gctgatcccg ccttcgccgc cgaggaggtt cagcacgacg aggggcgtgt ccgcgtactg
    16201 gcggacgtgc gggtgctccg tgttcacctt cccgaacgcg gccttgaact tgccggcctt
    16261 ggcgatgaag tcgcccggaa gcgtcaggaa ggtgatgaac ccctcctcga tcccgacgcc
    16321 gccctcgctg tcgagggaca cgaagaagtc ggcgcgcgcg tacggatcga cgatcgcctg
    16381 caaggcgatc tcggactcct tgagctggag gctcggctgg ttgtcgacgg agttgtgccc
    16441 gacgaacccg agcgcgttgc cgacgacgga gatgttcggg ttgaagacgt tgccggacgg
    16501 ggcgggcgcg gtctgcgcgg ccgccgaggc ggccgaaaga ctcaggagaa agaattctgc
    16561 gaaaaggaag aggggaacgg ccgcgcggcg gcctggattt tccttcaaag aaatctcctt
    16621 cttggagcgt gacggaagcc gaacggattc cgtgcggctc gacgacgacc tacgcggccg
    16681 aaggaggaga gcgtccggag taaccgggga gtacgggggc ggaagcggcg cgttccgcga
    16741 gcgcctcggg cagcaggcgc gacgccggcg gcgcgaggaa gacgggggcg gacgcgggcg
    16801 gcagcaggtt tgcgacgacg cacgccgcgc aggcgtgctc gggctgcggg atgaagggcg
    16861 cgtggttcgg atggtcgggc gcgaagagct gcgtgccgtg cgagagcgcc tcgatgccgt
    16921 cgacgtggcc gaacgccccg cccagcgcca gcggcgcgag cagggcgagg acggcggcgc
    16981 ggacgagacg ggctctcacc gcgggaaagt ctacgcctgc ggcccgctcc cgcctatttc
    17041 agcccggcca tcccgagata gctgccgtcg cccgtccccg cccccggcac cgcgccgtcg
    17101 ttgacgatgc cgtaggcgaa gaattgcagt gagtttcccg cgggagcgcg cacacgggcg
    17161 taacccgagc gaatccccag aggcgaaagc ggcgcgttca cctggatcca cgtcggagag
    17221 aacgagtcca cggagatcgt cctcgagccg gccgggaggc ccgtctcccc gttgaagaac
    17281 tccaaggtga gttcgacggg aagaggcgtg ggaggagaac ccgtgacggg cgggttcgtg
    17341 tttgccagcg cgagattgct gcgcgtcaac ccgtcctgct tcagcccgaa cagccaggtc
    17401 tccgagaagg cgacctctcc gacggcgagc gcggagtagg ccacgccgaa cgtcccgccg
    17461 gcggccgcgc gtgaggccgt ccgcgcgctc gcatggaacg ccgccgcacc gctcttcgaa
    17521 gaaacccgaa gcgccatcgc ctgagccgaa aagccgaggt acggaagcac gtcggagacg
    17581 aatcgctgct cctgcggctg gaggacggcg tcctccccgg tcgtggagcc ggggaaggaa
    17641 agcctcaccg gcacgtcacc ggcgttcgtc aggcagagct cggtcgtgaa gacgtccgtg
    17701 tgcaccgcga ccggaagcgt gaggacggtc gccgtacggc cggccgcgac cggcgccacg
    17761 aacgcgccgt ctccggtcac gttgtcgttc acgacgccgt acgcgtaaaa ggggtcggtc
    17821 ccgccgacgc gcgcgaccgt cacccatccg ttcgcatagc ccaccgccgc gagaacgcgg
    17881 ttcagctgcg tccattgccc gggtcccagc gtcacgtccg ggagcgtcac gctgcgcccg
    17941 tcacccgcgg cgccgctgcg cacggtcacc cgaagcgaca cggagccgcc cgtcgccgga
    18001 tccccggcgt tcaagagccc cacgttgctc cggaacgagg cgtcctcgcg cagcccgtag
    18061 agcgcgacag gcgcgtccag cgtctcgttg ggcgcgagcg ccgggtatgc gaggccggcg
    18121 cggccctccg gcagcaccgc gagagtccgc acggacgcga acgccgcgtc gccggaaacc
    18181 aggccggagt acgcgagacg cagcgtgccg ccctggtcgc cgagagggat gaagagcccg
    18241 ttctggcgga gccactgcag agcgtccgcg aagacgagct gccggcccgc ggcgaccgtc
    18301 gtcgtgaacg tgccgctccc acgcgagccg agcgcgtcgg acgccgtgaa cgtcacgctc
    18361 acggtcgcgt cggaggcccc gcggttcccc agcgtgagct cggagacgaa ccgcgcgccg
    18421 ctcgcgcccg aaacgtccac gacgaccggc accgtgagta tcgcgagcgg gccgtccgcg
    18481 tccacgagcc ggtagagcgt gccggagctg ccgtccacgg cgaggagctc gccgtcgtcg
    18541 ctctccccga agctcgtgaa cgcatacccc ggcctcagga gctccgccgt ctcccacccc
    18601 gtcgcgcctc tcctcaggcc ccagacccgc ccgctgcagt agtccgcgta gaagtagatg
    18661 ccgccgagcc gcgcgaagtc acgcccgcgg tagacgaacc cgcccgtgat cgagcagccg
    18721 ttcgcgtggg agtactccgc gaccgggggc gtcatccccg tggtgacgca tccggacggc
    18781 gggttgtagc agtgggcgcc ctcggtgacg cgccagccgt agttcgcgcc gccggcgaag
    18841 cccgcggcct ggaaatccac ctcctcccaa gcgccctgcc cgacgtcgcc gatgaagagg
    18901 tcgccggtct tgcgatcgaa ggagaaacgc cagggattcc tgagtccata agcccagatt
    18961 tcgggccgcg ccgttgaaga gcctatgaaa gggttagagg gcggaattgc atacggaacg
    19021 gtccctccca gaacgtccac tcgaagaatc ttccccagaa gggttccgag gctctgcccg
    19081 ttgttcaagg ggtcgccgcc cgaaccgccg tcccccatcc cgacgtagag atacccgtcg
    19141 ggcccgaacg cgagctgccc gccgttgtgg ttcgcgaacg gctgcccgac gctcaggatc
    19201 ttgacctcgg tcgacgcgaa cgcggcgtct cccgaaaagg agaaccgcga tacctgcgtg
    19261 ttcccggcga ggtccgtgta gttcgcccag aaccgcgtct tcggcccgcg cccgggctcg
    19321 aacgcgatcg agaggagccc gcgctcgccg cagcagctca cacggtcgcg aatgtcgagg
    19381 aacggggccg ggaggacggc gccatccttc acgatccgca cgagcccccg ctgctccacg
    19441 acgaagagcc ggccggagcc gtcgcccgcc gacgtgacct gcgtcgggct cgcgaacccg
    19501 gtcgtgaccc gtgtcagggc gacgttcggc cacccggcgg cggcgagaac ggacgcgagc
    19561 accacggaca ccatgcgccc tccccgaccg tagtggcccc gggcccgccc gtcctacgcg
    19621 aagtgacgaa gcgcggcggt ctcgtcgtcg tacacctcga agaccggcag gagctcggaa
    19681 aggtgcaggc tcttgcgcac ccgctcgtgg aggttcagga gcttcaggtt cgcgtcgaac
    19741 ttcccgcaga tcttcaggct cgcgacgagc tcgcccgtgc cggaactgtc gatcgcggtc
    19801 acgcccgaga ggttcaggag gatcttcttc cagccctcgg cgaggagctc gttcacgacg
    19861 ttccgcagga tctcgtcgcc gacaccggcg aggagccgcc ccttgaggtc gacgatcacg
    19921 acgtctccga ccttgcggac ttccacgtgc atggcgcgca tcgtaccgcg aacgcggggg
    19981 gtggcagaat cggcctccca tgcctgcgac cactctccac gagggcctgg aggctctgcg
    20041 cgccgaccgc gcgtccgagc ccgccgccct ctccgcggtc tcccgtctcg cctcggagtt
    20101 cggcactcct ctctacgtgt acgacaaggc cgcgatcacg ggccgcttcc gggccctgaa
    20161 ggccgcgttc gcgccgcact tcccgaagct gcgcctccac tacgccctca aggcgaacgg
    20221 cagcctcgcg atcggccgca tcctgcgcgc cgaaggcgcg ttccccgagg tcgtctcgct
    20281 cggcgagatc ctcaccgcgc tccgcgcggg gtggagcggg gcggacgtcc tcttcacgag
    20341 ttcctcgaag acgcccgagg agatcgcgaa gacgatcgag ctcggcgccg tcctgaacgc
    20401 ggactcgcgc gacgagctcg aacaggcgtc cgcggccgcg gtgaaggcgg gccgcacggt
    20461 gcggctctcc ttccgcctca acccgggcgt cgacccggac acgctccacc acatcaacac
    20521 gggcatcccc gagtcgaagt tcggcgtcca cctcgacggc ggcgacgcgc tcgccgcgta
    20581 cgcgcgcgcg aaggagctgc ccggcgtcgc gatcgcgggc atccactgcc acatcggctc
    20641 gcagatcgcg gacacggcgg gctacgagaa gacggccttg aagatgctcg cgttcgcgaa
    20701 ggagctgaag gaccggctcg ggatcgtcct cgagttcatc gacctcggcg gcggcttcgg
    20761 cgtcccctac cgggacggcg aatccgtcat gagccccgaa acgctcgcgg ccgcgctcga
    20821 gccgatctgg aaggaaggcg tggcggcggc gggctacgag cccgcgctgt ggctggagcc
    20881 ggggcgctcc ctcgtcgcgc cgtcgggctt cctcgtcacg cgcgtgaaca cggtcaagac
    20941 gacgccgctg aagacgttcg tcaacgtcga tgcgggctcg aacacgctcc tgcgcccggc
    21001 cctctacggc gcgtaccacc gggcgcgcgt cgtcgccgcg gccccgggcg cgccgcgcct
    21061 cctcgacgtc gcgggcgacg tctgcgagac cggcgacatc ctcgccgagg agcggacgct
    21121 gccgctcccg aaagccgggg accacgtcgt cttcctcgac gcgggcgcgt acggcttcgc
    21181 gatggcgtcc gtctacaacg cccggccgct tccggccgag gtgctcgtgg acggcgacgc
    21241 ggcgaccctg atccgccggc gcggaacgta cgacggtctt ttccgggacg aagtctccga
    21301 ctgaacgagg tgtccatgcg ccgaatctct ccgaccgccg ccgtccttct cgcgtcgctg
    21361 ctcgccgggt gcggggcgaa cccgttcccg gcgcagaccg tcgacttcgg gcagctgcag
    21421 cccgagccgc cgcgtgccct ctcgatgccg ctcgcggaca aggtcggcca gctcttcgtg
    21481 gtccaggcga acggcgtctt catgagcgag gactccgagc cgttccagac gctccggcac
    21541 cacgtcgtcg acaaccggat cggcggagtc atgctctccc gctccagcgt ctacggcgcc
    21601 gccgtcctcg tccagaaact gcaggaaatg gcccgtacgc cgctcctcgt ctcggccgat
    21661 ctcgaggccg gctcgggcat gcgcttcgag gacacgacgt acggcccgtg ggcgatggcg
    21721 atcgcggcga cgggcgaccc gtccctcgcc gagcgccgcg gccgggcgac cgccgaggag
    21781 gcccgcgcga tcggcgtcgc gcaggtctac gcgcccgtcg cggacgtgaa cgtgaacccg
    21841 gacaaccccg tcatcaacgt ccggagcttc ggcgaggacc cctccgacgt cgcgcgatac
    21901 gtcgtcgcga ccgtccacgg gctgcaggcc ggccaggtcc tcgcgacgct gaagcacttt
    21961 cccggccacg gcgatgccgc gacggattcc caccggtcgc tcgtcacgat ccccggcgac
    22021 cgggcgcgcc tcgaaagcgt cgagctcgtc ccgttccgcg ccgggatcaa ggccggcgcc
    22081 gaatccgtca tgatcgcgca cgtgtcggtc ccatcctggg acgcgacgcc ggcgccgatc
    22141 ctcgcgcatc cggtcaagcc ggcggacgtc atcggagaga cgttcgagat cgagaagggt
    22201 cccgtgccgg ctaccgtgtc ggcgccgatc gtcaccggga tcctccggaa ggaactcggc
    22261 ttcacgggcc tcgtcgtgac cgacgcgatg cggatgggcg gcatcacgtc ccactacgag
    22321 ccgggcgagg ccgcgattct cgcgatcctc gcgggctcgg accagatcct cctgtcgagc
    22381 gacaccgacg cggcgatcaa ggccgtcatg gccgccgtga agtcgggccg gatctccgag
    22441 gcgcggatcg acgagagcgt caagcgcatc ctcgacgcca agaagcgcct gaacctctac
    22501 gacaagggaa gtccgttcgt cgggaagatc tcgaagatcg tcggaaccca ggcgcacgtg
    22561 gacctcgagg ccgagatcgc gcgccgctcg atgacgctcg tgcgggagaa ggccggcgcc
    22621 ctccccttcc gccgggacgc aaagatcctc tcgctcgtcg tcgccgacga gccgacgctc
    22681 tacggacccg cgggaacgct cgacaaggag ctgaaaaccc gcgtcccggg cctgaagtcc
    22741 gtccgcctcg acacgcgctc gacgcccgag gaggcgaggg ccgccgccga cgccgcgaag
    22801 gacgccgacg ccgtgctcct ctcgctattc gtgcgcgcgc gctccgggca ggggaggttc
    22861 gtgatccccg acgcggcgag gtccgtgatc cccgcgctcc tcgcgtccgg aaagcccgtc
    22921 gtcgccgtcg cattcggcag cccgtacctc ctgcgcgact ttccggacct cccgacgtac
    22981 ctctgcgcgt ggggctcgca ggacgtcgtc caggtcgcgg cggcgaaggc gctgttcggc
    23041 gaggcgacga tcgacggccg cctgcccatc acgatcccgg ggctcgcgaa gaggggggac
    23101 gggatctcaa agccggtttt gcctcgctga gaccacggca gtttttcttt gaaggtgaag
    23161 aggcgcaccg gcctcgggag atgccgtggc ctgacccgct gcgacggccc ccctcaccct
    23221 tctaagaaaa tctccaataa tccccgcatg aatgctcttt gctcgctctc gcgcgctgcg
    23281 ttgttctctc tcgcttcggc cgcaatggcc tccgacgtcg acgacgctcg cgccctgttt
    23341 gagaggaatc tcgcggcgat ccagaagcgc gacaaggctg cgtacctcgc gtgctatctc
    23401 gacgggaaga cgctcgttcg cacgtcgccc gaggggttcg agctcggcta cgaggacttc
    23461 gcgaagcagg cgggagagaa gtggcccgac acgctcgccg ccgacgacct caggctcacg
    23521 cccgtctccc ccggcgtcgt ctacggcacg taccgctatc gcgtgcgtta cggcgccgag
    23581 gagcacgccg gcctctccga gcgcctcttc ctgaggacgc cgagggggtg gcgcatcgcc
    23641 gtgacctcgg cgtgggaagc tccagccggc acgccgccgc ctccgcgcgc attcacgggc
    23701 gcgacgctcg tggacgggac cggctccccc gcggtggcga acgccgtcgt cctcgtgcgg
    23761 aacggacgca tcgactgcgc ggggccggcc tccgcgtgcc ccgtgcccga aggcgtggcg
    23821 gtgaccgacg cgaagggctc gtggatcgtc ccgggtctcg tggacgcgca cgtccacttc
    23881 gcccagacgg gctgggccga cgggcgcccc gacgccttcg acgtgcgcgc gaagcacccg
    23941 tacgacgccg tccagaagga gctgcgcgag caccccgagc gtttcttccg ctcgtacgtc
    24001 tgctccggcg tcacctcggt cttcgacgtc ggcggctacg cgtggtccct cgacctcgcc
    24061 gcgcgggccg agaacgactc gctcgccccg cgcgtgagag cggccggccc gctcctctcc
    24121 acgatcgact tctggctgaa cctttccggc gagcggcagt tcatgttcct caaggacgcc
    24181 gagagcgcga aaacgggcgt cgcgtatctc gcctcgcgcg gggcatccgc ggtgaaggtc
    24241 tggtacgtcg tcacgcccga ccgccccgtc gaggcgtccg aggccgcggt cctcgcggcc
    24301 ggcgccgagg cgaaaaaggc cgggctgccg ctcatcgtcc acgcgaccgg cctcgcggag
    24361 gcgaagatcg ccctccgggc gggcgcgaag ctcctcgtcc acggcgtcac ggacaggccc
    24421 gtcgacgacg agttcctcgc gctcgcgaag caggccggcg cgacgtacaa ccccacgctc
    24481 gtcgtcctcg acggctacgt cgcggtcgcg aaggccgcgg ccgccggcgc cccctccccg
    24541 ttcgacgacc cgaacggctg cgccgatcct gcgtcgcgcg cgcgcctcgc ggccacgccc
    24601 gagatcgacg gcgcgtcgat cgccgcgaag gtgcgcgacc gggaaacgcg gaccgccgag
    24661 cggaaggccg tcggcctcgc gaacctcagg cgggtgcgcg acgcgggcat cccgatcgcc
    24721 atgggcacgg acgccgggaa cccgggaacg ctccacgggc cgtccgtcca cgccgagatg
    24781 gaagcgatgc agtcggccgg catgactccg atggaggtcc tcgtcgcgtc cacccgcggc
    24841 ggcgcgcgcg cgatgggacg ggacgacgtc ggcacgctcg agaagggaaa gcgcgcggac
    24901 ttccttctca tcggcgccga cccgctcctc gacgccgcga acctccgcaa gctgaacgcg
    24961 gtcgtccgcg gcggcgtcta ccgaacgcag gccgagctca gggcgatcgt cgcgagcgag
    25021 ccctgagcag ccgcggcgcg aggagcagcg tcacgccgta gaagacgaac gccgcggtca
    25081 gcgtcgcggg ccccccgacc cggagatcct cgatgaggag cttgacgccc gcggccgcga
    25141 ggagcgggta cgcgagaagc gagacgacgc cgaagcgcac gaaccgtacg gtccaggcca
    25201 ccacgagcgt ggcgccgcac agaacgacgg tgcgcgcgag cgccaggccc ggggccgtgg
    25261 acgtgaacgg cgcgagaccg accaccaggg cgagcgcgct tcccgaggcg acggccgcgc
    25321 tcacgagcac cgcccccggc cggggagccg gcgcgtcggg
//
LOCUS       DAOMFY010000027        25342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_632_length_25342_cov_6.862815, whole genome shotgun sequence.
ACCESSION   DAOMFY010000027 DAOMFY010000000
VERSION     DAOMFY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25342
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_632_length_25342_cov_6.862815"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1061)
                     /locus_tag="PLB01_04150"
     CDS             complement(117..1061)
                     /locus_tag="PLB01_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76524.1"
                     /translation="MTDAEDLRPFLARIGEAADRREMGLWLVGGAVRDLLLGRPTKDV
                     DLAVEAGAANALELASRLASLPGWSLKARHERFGTATLEAPGGVRVDLAATRREEYPA
                     PASLPVVTGTATIDEDLGRRDFTIHAMARRIGKRGLVGATLDPFGGKRDLAGKALRLL
                     HPKSLVDDPTRAYRAVKYAVRLGFTWDADFGKALKAARKADAFQALSGDRMRRGMEEI
                     FLEGEWGRAASLASGLALFGDVLPGWTGPSCPSKRSSSGVGGASASVDTNGPAEARPK
                     AEEIWRRLLEHLSGPERAAAAARLNFSRALAKAAGVPA"
     gene            complement(1058..4606)
                     /gene="dnaE"
                     /locus_tag="PLB01_04155"
     CDS             complement(1058..4606)
                     /gene="dnaE"
                     /locus_tag="PLB01_04155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQQ76525.1"
                     /translation="MSGDFVHLHLHTQYSLLDGANRVKDLIRNVKAAGMDAVAVTDHG
                     NMFGAYDLQTTALESGIKPIVGVEAYIAPGSRFDRETVKTFDGEGNNYHLTLLVETPE
                     GYKNLARLVSEAFLTGFYYKPRMDWELLEKHHDGIIALSGCLNGEVPKRLRAGDFEGA
                     KKVALKYQALFGKDRYFIEIMDHGLPEQRQILPDLLRLSQETGIPPVATNDSHYLTRD
                     DAAAQDVLLCIGTGKTLNDAKRMKFYNSEFYVKNPEEMSDVFRPYTLEAVRNTAAIAD
                     RITPSVIIDTGLKIPTYPVPVPDRNPDEYLEDLAKDGLERRLAHPAPYPAGSKPPKTR
                     AEYDERLAWELSVILKMGLSSYFLIVWDFIKYARDEGIPVGPGRGSAAGSLVAWALGI
                     TDVDPLRYDLLFERFLNPDRISMPDIDVDLCERRRGEVIEYVRRKYGRENVGQIITFN
                     SMKARAVIRDVGRVLDVPLAEVNRLCSLIPANPGKPTMLAEARRDVKELADALHDNET
                     YRRLFDLGERLEGVSRHAGVHAAGVLIAPRPLVEYLPLYRNSNDEITTQFEMKSVDRM
                     GLLKMDFLGLITLTILDDALAFVERKTGARPDLATIPLDDPEVYRLFADGRTDGIFQF
                     ESSGMRDLLRRVKPEVFDDLAALNALYRPGALDAGTVEVYIQRRRGTKFEYPLPQLEP
                     ILKETHGVLVYQEQVMLAARAVAGYSPGEADKLRKAIGKKDDALLKAEGDKFVRKAVA
                     AGTPKKKAEELWALILPFGRYGFNKSHSVVYALLAYRTAWMKVHHPVEFLAATLTASS
                     GSSDDVVKYVNTCREMKIPVLPPDVNKSELSFTPDGDAIRFGLGAVKGVGDGAVASVL
                     DARRAEGPFRSLTDFCSRVDLRLNNRRVIEALVKSGCFDTLGKTRAALLDGLDMAVDV
                     AAAAREAAASSQSLLFDVPKDAQVEDRFPDKPEMSADEKIRFEKETLGFYITGHPLTR
                     FEDEIRMFGDATCETLHERLDAPVKLVGLVSSIKKNQIKKGQNEGKMMAKAVVEDLTG
                     TVAVTVFASLLDRINGWFAPGKAVLVTGTVRSSMQIGAGATSGEHEGESSSMPIEVIA
                     REIQPLEGMKEQGAREVRIEPQNFETFDEATVIEILRKFPGSVPVSLELRRPGHFAAR
                     QKIDSRLWVRPTPEFTASMETLLGPGSVRYGYAPSA"
     gene            4640..5479
                     /gene="proB"
                     /locus_tag="PLB01_04160"
     CDS             4640..5479
                     /gene="proB"
                     /locus_tag="PLB01_04160"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQQ76526.1"
                     /translation="MDEARTRQRVTEARRIVVKAGTNVIMRDDGAVALGRLYGLIESV
                     ATLRKQGRDVLIVSSGAVGLGAQRLRLEKKPGSLALKQACAAIGQSRLMSVYEEGFEK
                     MGLSTAQVLLTEDDFADKARFSNLSATLEKLFELGVVPILNENDTVSTAELVTPDGTG
                     ARGFFGDNDRLSALVMSKVKADVLVILSDVDGLFTGNPAHDRTARLVPLVRDGDDVAR
                     YADGGGTRGRGGMASKLGAARIATRAGGIAVIANGRTPGILDRLFGGDAVGTLFVPAA
                     QRR"
     gene            5482..6831
                     /locus_tag="PLB01_04165"
     CDS             5482..6831
                     /locus_tag="PLB01_04165"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQQ76527.1"
                     /translation="MSEERKIFLEGEEGPVAIAKRARAAARSLAVLPSEKKDEILRSV
                     ADGLDAARDVILEANRADVLEARTTFKEIPSSSSSRSLLDRLVLDGEKVRAMSAGVRA
                     VAALPDPSGRILSRTLLDDGLVLEKVSCPIGVLLVVFESRPDAVPQIASLALKSGNAA
                     ILKGGRESARSTAAILDVFTGVYAEHAVPEGALQSVGGRADVDALLALDGEIDLVIPR
                     GGYDLVKHVMANTRIPVLGHAEGVCHVYVDRAADEAMATAIVLDAKLQYPAACNAAET
                     LLVHREAAAKFLVPLLTKLGDAGVELRACEGTRAFAPALALSPATEDDWRAEYGAPVL
                     AVKIVESLDEAVEHVNRYSSHHTEAIVTDDPSAAEAFLARVDAAGVFHNASTRFADGF
                     RYGFGAEVGVSTSRLHARGPVGLEGLVTYKYVLRGSGQVVATYSGKGARPFRHEPLP"
     gene            6828..7568
                     /locus_tag="PLB01_04170"
     CDS             6828..7568
                     /locus_tag="PLB01_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ76528.1"
                     /translation="MSAESVFVAGAAGAVGRVLVRLLVAGGHRVFGTTRSAERARELA
                     SAGVEPVVVDAFDADAVRRALLRAQPAALVHQLTDLPRVWDPATAPAALERNARLREV
                     GTRNLVAACAGLDVRRFVVQSIAFAYAPGPEPHAESDPLNVGAADPVAARTAGAVAFM
                     EEVALGGPFEGVVLRYGRLYGPGTWNATPPAGCAVHVDAAAHAALLALTRGAPGVYNV
                     AEPGGNVIVQRALEDLGWDPGFRAPGPG"
     gene            complement(7581..9119)
                     /gene="guaA"
                     /locus_tag="PLB01_04175"
     CDS             complement(7581..9119)
                     /gene="guaA"
                     /locus_tag="PLB01_04175"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQQ76529.1"
                     /translation="MSAASDTVVVLDFGSQYTQVIARRIREARVRSVVLPFDAKASEV
                     AKLAPKGIILSGGPSSVYDREAPQGDAGLFDLNVPVLGLCYGMMWLAQRYGGRVGSAP
                     GREYGRAHVKVSGGRLFRGLGPVETIWMSHGDHVESAPPGFSVTATTENAPVAAFEDA
                     SRNLYGIQGHPEVHHSEHGAQMLENFLYDVCGAKPSWTMAGFRDATIERLRGELTHGV
                     VLGALSGGVDSTVAAVLLREAVGERFRGVFVDTGLLRKNEGRQVLEAFRHLGLPVVGV
                     DASQRFFDGLAGVVEPERKRRIIGGLFIDVFTENAKAVDGAEWLAQGTLYPDVIESVS
                     IKGPSAVIKTHHNVGGLPEKLGFKLVEPLRELFKDEVRRLGEELGIPKEMLYRHPFPG
                     PGLAVRLPGEITPERVRILQEADAIFIEELKESGWYDRTSQAFAVLLPVKTVGVMGDG
                     RTYESVLALRAVTTEDFMTADWARLPHDLLDRVSRRVVGEVRGVNRVVYDVTSKPPGT
                     IEWE"
     gene            complement(9149..9679)
                     /locus_tag="PLB01_04180"
     CDS             complement(9149..9679)
                     /locus_tag="PLB01_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76530.1"
                     /translation="MFRTARAFLLVLPGAFLVVLSAYLVAHPGAADAFVAYPFLAESL
                     GSWVSKRPLIRFVLTSVVFFVPPYLVTGLLLFVADAGVSAAAPLWGEARKKRPGAGPP
                     PESRWGFVAVSLACAAAFGVLLHRVAHGGELPGGVNVSPLFVVIAAFGAVGVGLVAAG
                     LAALPRAVARRLGAAA"
     gene            complement(9679..10533)
                     /locus_tag="PLB01_04185"
     CDS             complement(9679..10533)
                     /locus_tag="PLB01_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQQ76531.1"
                     /translation="MSEEHGRKIFFEGEEGEGRSGFAPMGALAEASPTLLRKSSSLPL
                     FLFVVLAFLFAAAPAAATLVVFEDGRHVRVVSYEVVAGDGDGGDERVSMKLVGGGSMT
                     VSLDVVDRIVDDEYERLPAPKDGVPSAAETGASAGRSVRAFSENPSVSSPYSRQILEA
                     AKQHRVDPALIAAVIRAESNGVPTAVSRKGARGLMQLMPATARRLGVRSPFDPKENIR
                     GGTAYLAELAARYGDTRADLVLAAYNAGERAVDEHKGVPPFRETREYVRKVLAFWAGA
                     QRIAAASF"
     gene            complement(10544..11461)
                     /gene="xerD"
                     /locus_tag="PLB01_04190"
     CDS             complement(10544..11461)
                     /gene="xerD"
                     /locus_tag="PLB01_04190"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQQ76532.1"
                     /translation="MKPSDGVRGGVFLERHLPLFLDYLSAEKGLSPNTLDAYRRDLAG
                     FGAFLAERSVDAGKVSRGDVVAYLGARRAEGMSPRTLARETSALRGLYGFLAAEKVVP
                     EDPTADLTNARRWAMLPKTLSEDEVQKLLDAPDVATPRGLRDRAMFELLYACGLRVSE
                     LSALPIESLRLAEGFLLVRGKGSKERIVPVADSAGRWVERWLAEGRDTGGAGSRWVFP
                     GTKGRPVTRQTVFLALKAAAVKAGISAAAVSPHVLRHAFATHLVDHDADLRAVQLMLG
                     HADIATTEIYTHVSRSRVRKVYDRTHPRA"
     gene            complement(11463..12746)
                     /locus_tag="PLB01_04195"
     CDS             complement(11463..12746)
                     /locus_tag="PLB01_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HQQ76533.1"
                     /translation="MDRSRLVYDWNRAAGEGRPRHAPVELVDETLRDGLQSPSAVAPT
                     VAQRFELVCLMERLGVDTAVLGIPAAGPTARAEGEALARLVTEARLSLQPGFAARTLP
                     ADIEPVVEIAQRVGRVLEMTTFIGSSPIRQYVEDWDLSQMMKLTRDAVTMGVREGLLV
                     TYVTEDTTRARPEDLVALYGTAIQAGAKRICACDTVGHATPEGTGALVGFLAEFVARE
                     NPAVRIDWHGHQDRGLAVANSLSALRAGAHRVHGCGLGLGERVGNTPMDQMLVNLRLL
                     GWIDRDLSALAEYVQKVAEYVHVPIPDGYPVVGRDAFRTGTGGHAAAIVKARAKGDDW
                     LADRVYSGVPAGMFGLKQIIEVGPMSGESNVVTWMKERGLAAEPERVAAILRAAKLAN
                     HVLSDAEIRAILGSFPEAGKPGSARDASPAASVKH"
     gene            12809..13969
                     /gene="hppD"
                     /locus_tag="PLB01_04200"
     CDS             12809..13969
                     /gene="hppD"
                     /locus_tag="PLB01_04200"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HQQ76534.1"
                     /translation="MDSIGIVGYDSFHFVVTDLARSRRFYTEALGFTEVARAGREKVE
                     RGGEEASVFGAGQIRVLVSTPVRKDSRAARYLRIHPDGISSLAFRVKDVEAAWKFLDA
                     RGATFLSDIQTDETPNGAFRTFNIATPLDDVTFRFIQRDGDYAHFAPGFETVGDGKPK
                     ENPHGFATIDHVTSNAYTMLPVVNWYRDVLGFTHYWDVEFHTAEVASARPMAGSGLRS
                     IVMADPGSGVKFATNEPMRPFFRESQINRFCEDHHGAGVQHVAFLVKEIIPAVEALRA
                     KGIAFLPAPGAYYDRLPKRMAEVKISKLREPMELLRKNHILVDGADEKYMLQIFMLDA
                     MAMYGDETAGPFFYELIQREGDRGFGYGNFRALFESIESAQRSGSSALEVVG"
     gene            13966..15117
                     /locus_tag="PLB01_04205"
     CDS             13966..15117
                     /locus_tag="PLB01_04205"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQQ76535.1"
                     /translation="MIDWMRCGELPAKPHTAFRSPEGALRFEECLTRRGFDGEFSILY
                     HDGPPMTDSLIGPATPGRWDRKAPIPAEHQPLRRRLLLGEKAPEGFTPVLFNEDVAVS
                     LFRLPREAESPDGFSNGDGDDLFFFLEGEGTLETPFGDVAFRANDYLLVPRGVVARFR
                     ISSPLEGILFELRGGLHLPREFRNPVGQLRMDAPFTHRDFVRPEFKGPRSGPKRILVK
                     KDDAFVERFPVASPFDVVGWDGTVWPFAFDILKYQPRTGLIHLPPTIHSTFAGRNALV
                     CSFVPRVTDTHPDAIPCPYPHTSVDCDEIIYYVRGNFTSRKGVGPKAVSLHPAGVPHG
                     PHPGAYEGSIGTRSTDELAVMMDTFAPLRLTEQALALESPGYHDSWIPG"
     gene            15646..16014
                     /locus_tag="PLB01_04210"
     CDS             15646..16014
                     /locus_tag="PLB01_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HQQ76536.1"
                     /translation="MRSDVHQRLFTAGAEDAAQETYLSVTKALRSFRGDSSVSTWVYR
                     IAIRAALRVKAQLRAPHRPRPLLPRGPLPPPNRRNARDPRGNGPGSTSPGRSSRPSWT
                     RWASSEPETRGKRLRITSDA"
     gene            16028..17425
                     /locus_tag="PLB01_04215"
     CDS             16028..17425
                     /locus_tag="PLB01_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ76537.1"
                     /translation="MKPRLALAALALAAAIGPVAPAAEPPAPARAPIPVDYRKLPNGL
                     KVVLSPDHTSPIAVVAVYYNIGFRIEPRDRTGFAHLFEHLMFQGSKNLGKMEFIKLVQ
                     ANGGILNGSTRFDFTNYFEVVPANALKTALWAEADRMRGLAITQENLANQQGVVKNEV
                     KVNVLNRPYGGFPWLDMPQVANENWFNAHNFYGDLKDLDAATLKDAEAFFKAFYSPGN
                     AALVVTGDFETEATFKWIQEYFGPIPSAPHQELPDISEPRQEKEKRTSKQDAQAPRPA
                     LAVAYHAPKRNTPEYYALGLLEQILAGGSDSRLYDALVRRGALTGEVNSSINGLGNQF
                     NIAGPTLFTLSLFHDRDKTPDAILKVLDAEIEKLRTTPVDAATLERAQVKMRSDLYAM
                     QESTFGFGRADLLASFALFDDDPGRINGLEKEFAKVTPELIRKTAEEYLRPTNRTILA
                     IEPKPEAPKLPGPKV"
     gene            17437..18843
                     /locus_tag="PLB01_04220"
     CDS             17437..18843
                     /locus_tag="PLB01_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ76538.1"
                     /translation="MTIRKSFTLVFAAAFLALAPGLRADQPAVREKPPEPDAPRPFKI
                     PAPTRFSLPNGLKVSLVPYGTVPKALVRLTVHVGNADDPAGQTWMADLTGDLLLEGTK
                     TRSATRIAQDAASMGGEVAVQAGENLTTVGGEVLSEFAPAMVDLVADVVRNPAFPEAE
                     LARLKANLERRLSIAKSQSQQMAAEAFRAAMYPGQPYGRLFPAPDALKGYTLANAKAF
                     WAGHFGADRSALYVVGVFDAKATEAAIRKAFDGWGKAAAAAAPAPKPSAKRTLTVLDR
                     PGASQSTVILGMPVVDPSSPDYVPLVVTNALLGGSFGSRITSNIREQKGYTYSPFSQV
                     SVRRQGAYWAETADVTTNVTGASIKEIFGEIDRLRAEPPSAAELKGIQNYLAGTFVLQ
                     NSSRIGIANQLQFLALHGLPDSYLSGFVSRVHAVTPDEVTRIAKAYIRPEQTTLVVVG
                     DRKTIDDQLKPYAPPAQP"
     gene            complement(18864..19640)
                     /locus_tag="PLB01_04225"
     CDS             complement(18864..19640)
                     /locus_tag="PLB01_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQQ76539.1"
                     /translation="MSVVLTEVSERILTVTVNRPEKLNALNGEVLDALRAAFAVAKEN
                     PDVGAVILTGAGEKAFVAGADIAEFKAFTPVGARDFVRKGQATFDLIENLGKPVVAAV
                     NGFALGGGCELAMAATFRYASKNAKLGQPEVNLGLIPGYGGSQRLPRLVGKGRALELL
                     LTGDAIPADEAFRIGLVNRVVEPAELLPACRDTLKKILGKAPVALRYVLDAVNAGLDM
                     PFAAAEEHEATLFGLCAATEDMKEGVSAFLEKRPAKFAGR"
     gene            complement(19645..20145)
                     /locus_tag="PLB01_04230"
     CDS             complement(19645..20145)
                     /locus_tag="PLB01_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ76540.1"
                     /translation="MIDPMLMEFDRESSTTRKLLSRVPADKYSWKPHTKSMSLGALAG
                     HIAGLPHWIAGSLLPGEFDLATGGDGTAKEPASAEALMKTFEESAAGAKAAMAQLDDV
                     RAMGPWTLRHGTKVIMEMPRIAFVRTILLNHLIHHRGQLSVYLRLLDVPLPSIYGPSA
                     DENPFG"
     gene            complement(20155..21030)
                     /locus_tag="PLB01_04235"
     CDS             complement(20155..21030)
                     /locus_tag="PLB01_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019526167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HQQ76541.1"
                     /translation="MSEIRKVGILGAGLMGSGIAEVCARAGFTVVVREPTEELLAKGR
                     MRVTNSLSKAKQRGKLNDAEEIAIASRLTFSSSLEELSSSDLIVEAIVENLDVKREVY
                     QALDALCGPETIFCSNTSSLTITEMAAATKRPDRFAGLHFFNPVPVMKLAEVVRGLAT
                     SDATVAAVTAFARALGKEPVACRDNSGFIVNRLLVPFLLDAIRAHEQGVGSIEDIDTG
                     MKLGCAHPMGPFELLDYVGLETVYAIAEIMFAEYRETRFAPPALLKRLVLAGRFGRKT
                     GRGFFEYDEKGNRKG"
     gene            complement(21027..22214)
                     /locus_tag="PLB01_04240"
     CDS             complement(21027..22214)
                     /locus_tag="PLB01_04240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQQ76542.1"
                     /translation="MASPSIVVAEGARTPMAEYNGLFADMTATDLAVVAAQEALRRAR
                     FAPGEIGHVIVGNVLQTSSDAIYLSRHVGLRAGVPQEVPAVTVNRLCGSGFEALVQAK
                     HRILLGEAEAVLAGGTENMSQAPFVVRGARTGLRLGQGKLEDSLMSGLFDTVPGLAMA
                     QTSDNVARRHGITREEQDAFALESQKKAADAWASGRLAEEVVPVEAKKGRKTVTVSKD
                     DHMTPDTTLEGLAKLSPAFGKDGMVTGGNASGIVDGAAMMVVTTEERARAKGIVPLGR
                     LVAEAVTGCDPAFMGLGPVAASRAALAKAGKSLADMDLVEINEAFAGQILGVMKELGV
                     DRAKLNVNGGAIALGHPLGASGARLTLTLLKELRRRGQRWGLASACIGGGQGIAVVVE
                     ALS"
     gene            complement(22310..23413)
                     /locus_tag="PLB01_04245"
     CDS             complement(22310..23413)
                     /locus_tag="PLB01_04245"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQQ76543.1"
                     /translation="MSAPDSLPSLLDEGLERGFFAGASALVVSEKETLAERHAGDARV
                     EPASERRAAGPDTLWDLASLTKPLAGAALVLSLAGENALSLDDTVWRFEDRWRKSFLE
                     GVSVRRLLAHLGGIVDWFPVYSRGEGRAAYARTLGELDAAAIPGTKVIYSCPGYLFLS
                     EIVERVSGGTLDRLFEARVSAPLHLSKNLLFSPSSPEDLARTAGGERDDATERAMTAD
                     RKLKYGGFRQGVVNGQVNDGNAFRRGNGVSLNAGLFGTAEAAAAVGRAWLARDGRLLP
                     ERFFALATENATAGLGEDRGLGWQLASTPGSAGEPLGPSAYGHTGFTGASLFVAPGLG
                     RVAVLLTNRLHPDARAAEMNAFRRRFHEIAAGL"
     gene            complement(23410..24078)
                     /locus_tag="PLB01_04250"
     CDS             complement(23410..24078)
                     /locus_tag="PLB01_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76544.1"
                     /translation="MFGRRVAFLPPFFAIAVLSGCGGSDAPTAPTPAAGVSRPGVSRD
                     VYRPPVDGRVTPKQVETYLGVLDRVRLDRKAGKAPPPSDPLASESADVTVARARNVNT
                     EEYLWVKERVLEAEAAAMTVRLNASALVMLDKTLADLRARKADAADEGSRKLINEQIA
                     NFEAEAERVRRESREKEPDSVRANMKTLEPFRARLAAAHEALDRPLLPSAAKEPSKAP
                     TGKP"
     gene            24119..>25342
                     /locus_tag="PLB01_04255"
     CDS             24119..>25342
                     /locus_tag="PLB01_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76545.1"
                     /translation="MLTQVGKYLIEEKVGVGGFGTVYKGRDPFIKRAVAIKTCQSDEE
                     EIKKRFFREAEFAGNLHHRNITTIYDFGVTDDGTPYIVQEFLTGEDLDKLARKRSPLP
                     LRRKLEILVDVCEGLGYAHTAGIIHRDIKPSNVRILEDGTVKLMDFGIAKSMVSQSTL
                     TQTGITLGTASYLAPEQIRGEDLDARTDLFSLGVLAYELTTGQKPFTGDHISTVLYRI
                     MNEQPAPPSTLVPGLPRALDAILVKLLEKDRTRRHANCAEVKAELSAVLAGLSDAPEH
                     DAPAEDMDRTVATPSAGYQTGVAARPARVLDTPLSPSAPLRQTQGGAADVRLRATGGA
                     PILPPPAAEAHARESGPVLRVLLSAAVVVLAGGGGFWLYVQKKSAKPQPPASADAAAP
                     AATAAATTAPSTGSTA"
BASE COUNT         3829 a         9095 c         8564 g         3854 t
ORIGIN      
        1 gcggatctcg ggggtccggg tcggcgtcgc cggggcggtc ggcgtcggcg tgggcgacgg
       61 ctcgggcgcc gcgacgagca cgagcgcgac gtgcgcggcg aggagcagcg agaggctcat
      121 gcgggaacgc ccgccgcctt cgccagcgcg cgcgagaaat tgaggcgcgc ggcggcggcg
      181 gcgcgctcgg gccccgacag gtgctcgaga agccggcgcc agatttcttc tgctttcgga
      241 cgcgcctccg ccggcccatt cgtgtcgact gatgccgacg caccgccaac gccgcttgaa
      301 gatctcttag aagggcaaga ggggccggtc cagccgggga ggacgtcgcc gaagagtgcc
      361 agcccactcg cgagggacgc agccctcccc cattcacctt ctaagaaaat ctcttccatt
      421 cctcttctca tccgatcgcc cgagagtgct tggaatgcgt ccgctttcct ggcggccttc
      481 agcgccttgc cgaagtccgc gtcccaggtg aagcccaggc gcaccgcgta cttcaccgcg
      541 cggtacgcgc gagtcgggtc gtcgacgagc gacttcggat gcaggaggcg cagggccttg
      601 cccgcgaggt cccgctttcc gccgaaaggg tcgagcgtcg cgccgacgag gccgcgtttt
      661 ccgatgcgcc gcgccatcgc gtggatggtg aagtcccgcc gcccgaggtc ctcgtcgatc
      721 gtggccgtgc ccgtcacgac gggaaggctc gcgggcgccg ggtactcctc gcgccgcgtg
      781 gccgcgaggt ccacgcggac gccgccgggc gcctcgagtg tcgcggtccc gaaccgctcg
      841 tgccgcgcct tgagcgacca gcccggaagc gatgcgagcc gggacgcgag ctcgagcgca
      901 ttggccgcgc ctgcctcgac ggcgaggtcg acgtccttcg tcgggcgccc gagaagcagg
      961 tcgcgcaccg ccccgccgac gagccagagg cccatctcgc ggcggtccgc ggcctcgccg
     1021 atacgggcca gaaaagggcg gaggtcttcc gcgtccgtca tgccgacggc gcgtagccgt
     1081 accggacgga tcccgggccg aggagagtct ccatcgaagc ggtgaactcg ggcgtgggcc
     1141 ggacccacag acgcgaatcg atcttctgcc gcgccgcgaa atgccccggc cgccggagtt
     1201 cgagcgagac gggcaccgag ccagggaact ttctgagaat ctctatgacg gttgcttcat
     1261 caaaggtctc gaagttctga ggctcgatgc gcacttcgcg ggcgccctgc tctttcatcc
     1321 cctcgagcgg ctggatctcg cgggcgatca cctcgatcgg catcgacgag ctctcgccct
     1381 cgtgctcccc ggaggtcgcg ccggcgccga tctgcatcga ggagcggacg gtcccggtca
     1441 cgaggacggc cttgcccggc gcgaaccagc cgttgatgcg gtccagcagg ctcgcgaaga
     1501 ccgtgacggc cacggtgccc gtgaggtcct cgacgacggc cttcgccatc atcttcccct
     1561 cgttctgccc cttcttgatc tggttcttct tgatcgagga cacgaggccg acgagcttga
     1621 cgggcgcgtc gagccgctcg tggagcgtct cgcaggtcgc gtccccgaac atgcggatct
     1681 cgtcctcgaa ccgcgtgagc ggatgccccg tgatgtagaa cccgagcgtc tccttctcga
     1741 agcggatctt ctcgtccgcg ctcatctcgg gcttgtccgg gaagcggtcc tccacctgag
     1801 cgtccttcgg gacgtcgaag agaaggctct ggctcgacgc cgcggcctcg cgcgcggccg
     1861 cggccacgtc gaccgccatg tcgaggccgt cgagcagggc cgcgcgggtc ttcccgagcg
     1921 tgtcgaaaca cccggacttc acgagcgcct cgatgacgcg ccggttgttg aggcgcaggt
     1981 cgacgcgcga gcagaagtcc gtcagcgacc ggaacggccc ctcggcccgg cgcgcgtcga
     2041 gcacggacgc cacggcaccg tcgccgacgc ccttcacggc gccgagcccg aaccggatcg
     2101 cgtccccgtc gggcgtgaag gacagctccg acttgttcac gtccggcgga aggaccggga
     2161 tcttcatctc gcggcacgtg ttgacgtact tgacgacgtc gtcggagctg cccgacgagg
     2221 ccgtcagcgt cgccgcgagg aactccacgg gatggtggac cttcatccac gccgtgcggt
     2281 acgcgagcag cgcgtagacg accgagtggg acttgttgaa cccgtagcgg ccgaacggca
     2341 ggatgagcgc ccacagctcc tcggccttct tcttcggcgt gccggccgcg acggccttcc
     2401 tgacgaactt gtccccctcg gccttgagga gcgcgtcgtc cttcttgccg atcgccttcc
     2461 ggagcttgtc ggcctctccg ggcgagtacc ccgccaccgc gcgcgccgcg agcatgacct
     2521 gctcctggta gacgaggact ccgtgcgtct ccttgaggat cggctcgagc tgcggcaggg
     2581 ggtactcgaa cttcgtgccc cgtcgccgct ggatgtacac ctcgacggtc cctgcgtcga
     2641 gcgcgcccgg tcggtagagg gcgttgagag cagcgaggtc gtcgaacacc tcgggcttca
     2701 cgcgccgaag gaggtcgcgc atcccggacg actcgaactg gaagattccg tccgtccgcc
     2761 cgtccgcgaa cagccggtag acctcggggt cgtcgagcgg gatcgtcgcg aggtccggcc
     2821 gcgccccggt cttcctctcc acgaacgcga gcgcgtcgtc gaggatcgtg agcgtgatga
     2881 gcccgaggaa gtccatcttg aggagcccca tccggtccac ggacttcatc tcgaactggg
     2941 tcgtgatctc gtcgttgctg ttccggtaga gcggcaggta ctcgacgagg ggcctcggcg
     3001 cgatcaggac gccggccgcg tggacgcccg cgtggcgcga cacgccctcg aggcgctctc
     3061 cgaggtcgaa gaggcgccgg tacgtctcgt tgtcgtgcag ggcgtcggcg agctctttca
     3121 cgtcccggcg cgcctcggcg agcatcgtcg gcttgcccgg gttcgccggg atgagcgagc
     3181 agaggcggtt cacttccgcg agcggcacgt cgaggacgcg gcccacgtcg cggatcaccg
     3241 cgcgggcctt catcgagttg aaggtgatga tctgcccgac gttctcgcgc ccgtacttcc
     3301 gccggacgta ctcgatgacc tcgccgcgcc ggcgctcgca gaggtcgacg tcgatgtcgg
     3361 gcatcgagat gcggtccggg ttgaggaaac gctcgaagag gaggtcgtac cgcagcgggt
     3421 cgacgtccgt gatcccgagc gcccaggcga cgagcgaccc ggccgccgat ccgcggcccg
     3481 gaccgaccgg gatcccctcg tctctggcgt acttgatgaa gtcccagacg atgaggaaat
     3541 agctcgagag ccccatcttc aggatgacgg aaagctccca cgcgaggcgc tcgtcgtact
     3601 cggcccgcgt cttcggcggc ttcgacccgg ccgggtaagg cgccgggtgc gcgaggcgcc
     3661 tctcgaggcc gtccttcgcg aggtcctcga gatactcgtc cgggttccgg tcgggcaccg
     3721 ggaccgggta cgtcgggatc ttcagtcccg tgtcgatgat gacgctcggc gtgatccggt
     3781 ccgcgatcgc cgcggtgttc cgcacggcct cgagcgtgta gggccggaac acgtcgctca
     3841 tctcctcggg gttcttcacg tagaactccg agttgtagaa cttcatgcgc ttggcgtcgt
     3901 tgagcgtctt gcccgtgccg atgcagagca ggacgtcctg cgccgccgca tcgtcgcgcg
     3961 tgaggtagtg cgagtcgttc gtcgcgaccg gcggaatgcc cgtctcctgc gacaggcgca
     4021 gcaggtcggg cagaatctgc cgctgctcgg gcaggccgtg gtccatgatc tcgatgaagt
     4081 agcggtcctt cccgaacagg gcctggtact tcagcgcgac cttcttcgcc ccctcgaagt
     4141 cgccggcgcg gaggcgcttc gggacctcgc cgttcaggca gccggagagc gcgatgatcc
     4201 cgtcgtggtg cttctcgaga agctcccagt ccatccgcgg cttgtagtag aagccggtga
     4261 ggaacgcctc ggagacgagc cgcgcgagat tcttgtagcc ctcgggcgtc tcgacgagca
     4321 gcgtcaggtg gtagttgttc ccttcgccgt cgaacgtctt gacggtctcg cggtcgaaac
     4381 gcgagccggg cgcgatgtag gcctcaacgc cgacgatggg cttgatcccc gactcgaggg
     4441 ccgtcgtctg gaggtcgtac gccccgaaca tgttcccgtg gtccgtcacg gccacggcgt
     4501 ccattcccgc cgccttcacg ttgcggatga ggtccttgac gcggttggcg ccgtcgagga
     4561 gcgaatactg ggtgtgcagg tggaggtgaa cgaagtcgcc ggacatccgg gccggattct
     4621 agcgtcctac aatcgaggga tggacgaagc tcgaacccgg cagcgcgtca ccgaggcgcg
     4681 gcggatcgtc gtcaaggccg ggacgaacgt catcatgcgc gacgacggcg ccgtcgcgct
     4741 cgggcgcctg tacggcctca tcgagtcggt tgcgacgctc aggaagcagg ggcgcgacgt
     4801 cctgatcgtg tcctccggcg ccgtcgggct cggcgcgcag cgcctgcgcc tcgagaagaa
     4861 gcccggctcg ctggccctca agcaggcgtg cgcggccatc gggcagagcc gcctcatgtc
     4921 cgtctacgag gaaggtttcg agaagatggg cctctcgacg gcccaggtcc tcctcaccga
     4981 ggacgacttt gccgacaagg cgcggttctc gaacctgtcc gccacgctcg agaagctctt
     5041 cgagctcggc gtcgtcccga tcctcaacga gaacgacacc gtctcgaccg ccgagctcgt
     5101 caccccggac ggcacgggcg cgcgcggctt cttcggcgac aacgaccggc tcagcgccct
     5161 cgtcatgagc aaggtgaagg ccgacgtcct cgtgatcctc tcggacgtgg acggcctctt
     5221 caccggcaat cccgcgcacg accgtacggc gcgcctcgtg cccctcgtgc gtgacggcga
     5281 cgacgtggcg cggtacgcgg acggcggcgg gacgcgcggc cgcggcggca tggcctccaa
     5341 gctcggcgcc gcacgcatcg cgacgcgcgc cggcggcatc gccgtcatcg cgaacgggcg
     5401 gacgccgggg attctggatc ggctcttcgg gggggatgcg gttgggacgc tcttcgtgcc
     5461 ggcggcccag cgcaggtaga gatgtccgaa gagaggaaga tcttcttaga aggtgaggag
     5521 gggccggtgg cgatcgcgaa gcgcgcccgt gccgcggctc gcagcctcgc cgttctgccc
     5581 tcggagaaga aggacgagat cctaagaagc gtcgcggacg ggctcgatgc ggcgcgggac
     5641 gtcatcctcg aagcgaaccg ggcggacgtg ctcgaggccc gcaccacctt caaggaaatt
     5701 ccctcttcct cttcctcccg ctcccttctc gaccgcctcg tcctcgacgg ggagaaggtc
     5761 cgcgcgatgt ccgccggcgt ccgcgccgtc gccgccctcc ccgacccgtc cggccgaatc
     5821 ctttcccgga cgctcctcga cgacgggctc gtcctcgaga aggtctcgtg ccccatcggc
     5881 gtgctcctcg tggtcttcga gtcgcgcccc gacgcggttc cgcagatcgc ctccctcgcg
     5941 ctcaagtccg gcaacgcggc gatcctcaag ggcggacgcg aatccgcgcg ttcgacggcc
     6001 gcgatcctcg acgtcttcac cggcgtctac gcggagcacg ccgtgcccga gggcgcgctg
     6061 cagtccgtcg ggggccgggc ggacgtggac gccctcctcg cgctcgacgg cgagatcgac
     6121 ctcgtgatcc cgcgcggcgg ttacgacctc gtgaagcacg tcatggcgaa cacgcgcatc
     6181 ccggtcctcg ggcacgcgga aggcgtctgc cacgtctacg tggaccgcgc cgcggacgag
     6241 gcgatggcga cggcgatcgt ccttgacgcc aagctccagt acccggccgc gtgcaatgcg
     6301 gctgaaacgc tgctcgtcca ccgcgaggcc gccgcgaagt tcctcgttcc cctgctcacg
     6361 aagctcggag atgccggagt ggagcttcgc gcctgcgagg ggacccgcgc cttcgcgccg
     6421 gcgctcgccc tctcccccgc gaccgaggac gactggcgcg ccgagtacgg agcgcccgtc
     6481 ctcgcggtga agatcgtcga gtctctggac gaggccgtcg agcacgtgaa ccgctacagc
     6541 tcgcaccata cggaggcgat cgtgacggac gatccctccg ccgccgaggc gttcctcgcc
     6601 cgcgtggacg ccgccggcgt cttccacaac gcctccacgc gcttcgccga cggcttccgc
     6661 tacggcttcg gcgcggaagt cggagtctcc acgtcccgcc tccatgcccg ggggccggtc
     6721 ggcctcgagg ggctcgtcac gtacaagtac gtcctcaggg gcagcggcca ggtcgtcgcg
     6781 acgtactccg gcaagggcgc ccggcccttc cggcacgaac cgctgccgtg agcgcggagt
     6841 cggtctttgt cgccggagcc gcgggcgccg tcggccgcgt cctcgtccga ctgctcgtcg
     6901 ccggcgggca ccgcgtcttc ggcaccacgc gctcggccga gcgggcacgg gaactcgcga
     6961 gcgccggggt cgaacccgtc gtcgtggatg ccttcgacgc ggacgccgtc cgccgcgccc
     7021 tcctccgcgc tcagcccgcg gctctcgtcc accagctcac ggacctcccc cgcgtctggg
     7081 accccgctac ggcccccgcc gccctcgagc gaaacgcccg cctccgcgag gtcggcacgc
     7141 ggaacctcgt cgccgcctgc gccggcctcg acgtccgccg gttcgtggtc cagagcatcg
     7201 ccttcgccta cgcgcccggc cccgagcccc acgccgagag tgatccgttg aacgtcggcg
     7261 ccgcggatcc tgtcgccgcg cgcacggccg gagccgtcgc gttcatggaa gaggtcgctc
     7321 tcggggggcc gttcgagggc gtcgtcctgc gctacgggcg actctacgga ccgggaacgt
     7381 ggaacgcgac gccgcctgcg ggctgcgccg tccacgtgga cgccgccgcg cacgcggccc
     7441 tcctcgccct cacgcgcgga gcgccgggcg tctacaacgt cgccgaaccc ggcgggaacg
     7501 tcatcgtgca gagggcgctc gaagacctcg gctgggaccc cgggtttcgc gccccgggcc
     7561 cgggctgatt ccgcccggcc tcactcccac tcgatcgtgc cggggggctt cgacgtgacg
     7621 tcgtagacga cgcgattgac gccgcggacc tcgcccacga cgcgccgtga gacgcgatcg
     7681 aggaggtcgt gcgggaggcg cgcccagtcg gccgtcatga agtcctccgt cgtcacggcg
     7741 cgcagcgcga ggacgctctc gtaggtccgg ccgtcgccca tgacgccgac cgtcttcacg
     7801 ggcaggagca ctgcgaacgc ctgggacgtg cggtcgtacc agccggattc tttcagttcc
     7861 tcgatgaaga tcgcgtcggc ctcctgcagg atgcgcacgc gctcgggcgt gatctcgccc
     7921 gggaggcgca ccgcgagccc cggtcccggg aacgggtgcc ggtacagcat ctcctttggg
     7981 attcccagct cctcgccgag cctccggacc tcgtccttga acagctcgcg gagcggctcg
     8041 acgagcttga acccgagctt ctcgggcagg cccccgacgt tgtggtgcgt cttgatgacg
     8101 gccgacgggc ccttgatcga cacggactcg atgacgtccg ggtagagcgt gccctgggcg
     8161 agccactcgg ccccgtccac ggccttcgcg ttctccgtga agacgtcgat gaagaggccg
     8221 ccgatgatcc ggcgcttgcg ctcgggctcg acgacgccgg cgaggccgtc gaagaaccgc
     8281 tgcgatgcgt ccacgccgac gaccgggagg ccgaggtggc ggaacgcctc gaggacctgc
     8341 cggccctcgt tcttccggag gagtcccgtg tccacgaaga cgccgcggaa cctctccccg
     8401 acggcctcgc gcaggagaac ggccgcgacc gtggaatcga cgccgcccga gagcgccccg
     8461 aggacgacgc cgtgcgtcag ttccccgcgc aggcgctcga tcgtcgcgtc gcggaagccc
     8521 gccatcgtcc acgacggctt cgcgccgcag acgtcgtaga ggaagttctc gagcatctgg
     8581 gccccgtgct cggagtggtg gacctccggg tggccctgga tgccgtagag gttccgggac
     8641 gcgtcctcga acgccgcgac gggggcgttt tccgtcgtgg ccgtcacgga aaagcccggg
     8701 ggcgcggact ccacgtggtc gccgtggctc atccagatcg tctcgaccgg gccgaggccg
     8761 cggaagaggc gaccgccgga gaccttcacg tgggcgcgcc cgtattcgcg acccggcgcg
     8821 ctcccgacgc ggccgccgta gcgctgggcg agccacatca tcccgtagca gaggccgagg
     8881 accggcacgt tcaggtcgaa gagcccggcg tcgccctgcg gcgcttccct gtcgtagacg
     8941 ctggacggcc cgcccgacag gatgatcccc ttcggcgcga gcttcgcgac ttcgctggcc
     9001 ttcgcgtcga acggcaggac gaccgagcgg acgcgcgcct cgcggatccg ccgggcgatg
     9061 acctgcgtgt actgggagcc gaaatcgagg acgacgacgg tgtcgctggc ggcgctcacg
     9121 ccgcggagta tagaggcgcg cgagcccctc aggccgcagc gccgagacgc cgcgcgacgg
     9181 cccgcggaag cgcggcgagg ccggccgcga cgagcccgac tccgaccgct ccgaacgccg
     9241 cgatcaccac gaagagggga gagacgttga cgcctcccgg cagctcgccc ccgtgcgcga
     9301 cccggtgcag gaggactccg aacgcggccg cgcaggcgag gctgaccgcg acgaaacccc
     9361 accggctctc gggcgggggc ccggctcccg gccgcttttt tctcgcctct ccccacagcg
     9421 gcgccgcggc cgacacgccg gcgtccgcca cgaagagtag aaggcccgtc acgaggtacg
     9481 gcgggacgaa gaacaccacc gaggtgagga cgaaccggat cagcggccgc ttgctcaccc
     9541 aggagcccag tgattccgcg aggaacgggt acgcgacgaa cgcgtctgcg gcgcccggat
     9601 gtgcgacgag gtacgcactc aggacgacga ggaacgcccc cggcaggacg aggagaaacg
     9661 cgcgcgccgt tcggaacatc agaaggacgc cgccgcgatg cgctgggcgc cggcccagaa
     9721 cgccaggacc ttccggacgt actcgcgcgt ctcgcggaag ggcggcactc ccttgtgctc
     9781 gtccacggcg cgctcccccg cgttgtaggc cgcgagcacg aggtcggcgc gcgtgtcgcc
     9841 gtatcgcgcg gcgagctccg cgaggtaggc cgtcccgccc cggatgttct ccttcgggtc
     9901 gaacgggctc cgaacgccga ggcggcgcgc cgtcgccggc atgagctgca tgagcccgcg
     9961 cgcgcccttg cgggacacgg ccgtcggaac gccgttcgac tcggcccgga tgaccgccgc
    10021 gatgagggcg gggtcgacac gatgctgctt cgcggcttcg agaatctgcc tcgaatacgg
    10081 ggaggagacc gagggatttt ctgagaaggc gcggacggac cttcccgcgg aggcgcccgt
    10141 ctcggccgcg gaggggacgc cgtctttcgg ggccgggagg cgttcgtatt cgtcgtccac
    10201 gatccggtcg acgacgtcca gcgacacggt catcgagccg ccgccgacga gcttcatcga
    10261 gacgcgctcg tcgcccccgt cgccgtctcc cgccacgacc tcgtacgaca cgacgcgcac
    10321 gtgccgcccg tcctcgaaga cgacgagcgt ggcggcggcg ggagccgccg cgaagaggaa
    10381 tgcgaggacg acgaagagaa agaggggaag agaagaagat tttcttagaa gggtgggtga
    10441 ggcttcggca agagcgccca tcggggcgaa accggaacgg ccctccccct cttcaccttc
    10501 aaagaagatc tttcttccgt gctcttcgga catgaccctg ttgctatgcc ctggggtgcg
    10561 tccggtcgta gaccttgcgc acgcgcgagc gagaaacgtg ggtgtagatc tcggtggttg
    10621 cgatgtcggc gtggccgagc atgagctgca cggcccggag gtcggcgtcg tggtctacga
    10681 ggtgcgtcgc gaacgcgtgg cggaggacgt gcggcgaaac cgcggccgcc gagatgccgg
    10741 ccttcacggc cgcggccttg agcgcgagga agaccgtctg gcgtgtcacg ggccggccct
    10801 tcgttccggg gaacacccag cggctgcctg cgccgccggt gtcgcgcccc tccgcgagcc
    10861 agcgctcgac ccagcgcccg gccgagtcgg cgaccggcac gatgcgctcc ttgctcccct
    10921 tcccgcgcac gaggaggaag ccctcggcga ggcgcaggct ctcgatgggc agcgcggaga
    10981 gctccgagac gcgcaggccg caggcgtaga ggagctcgaa catcgcgcgg tcgcgcaatc
    11041 ccctcggcgt cgcgacgtcc ggcgcgtcga ggagcttctg gacctcgtcc tccgaaaggg
    11101 tcttcggcag catcgcccag cggcgtgcgt tcgtgaggtc ggccgtcggg tcctcgggca
    11161 cgaccttttc cgccgcgaga aagccgtaca gaccgcggag cgcggaggtt tcgcgcgcga
    11221 gcgtgcgcgg cgacatcccc tccgcgcggc gcgccccgag gtaggcgacg acgtcgccgc
    11281 gggacacctt tcccgcatcc acggatctct cggccaggaa cgctccgaac cccgcgaggt
    11341 cgcggcggta cgcgtcgagg gtgttcggcg agaggccctt ctccgccgag aggtagtcga
    11401 gaaacagcgg caggtgccgt tcgaggaaca cgcctccacg gacgccgtcg gaaggcttca
    11461 cgtcagtgtt ttacggaagc cgcggggctc gcgtcgcgcg cgctccccgg cttgccggcc
    11521 tccgggaacg atcccaggat ggcgcggatc tccgcgtcgg aaaggacgtg gttcgcgagc
    11581 ttcgcggcgc ggaggatcgc cgcgacgcgc tcgggctcgg ccgccagccc gcgctccttc
    11641 atccacgtca cgacgttgga ctcccccgac atcgggccca cctcgatgat ctgcttgagc
    11701 ccgaacatcc cggccggcac gccggagtag acgcggtcgg cgagccagtc gtcgcccttc
    11761 gcccgcgcct tcacgatcgc ggcggcgtgg ccacccgtcc cggtgcggaa cgcgtcgcgc
    11821 ccgacaaccg ggtacccgtc ggggatgggt acgtggacgt actccgcgac cttctggacg
    11881 tactccgcga gggccgacag gtcgcggtcg atccagccga ggaggcggag gttcacgagc
    11941 atctggtcca tcggcgtgtt tcccacgcgc tcgccgaggc cgagcccgca gccgtgcacg
    12001 cggtgcgctc cggcgcggag ggccgagagc gagttcgcca cggcgagccc gcggtcctgg
    12061 tggccgtgcc agtcgatcct cacggcgggg ttctcgcgcg ccacgaactc ggccaggaag
    12121 ccgacgagcg ctccggtgcc ttccggcgtc gcgtgcccca cggtgtcgca ggcgcagatc
    12181 cgcttcgcgc cggcctggat cgcggtgccg tagagggcga cgaggtcctc gggccgcgcc
    12241 cgcgtcgtgt cctcggtgac gtacgtgacg aggagaccct cgcgcacgcc catcgtcacg
    12301 gcgtcgcggg tgagcttcat catctgagag aggtcccagt cctcgacgta ctgccggatg
    12361 ggcgacgacc cgatgaaggt cgtcatctcg agcacgcggc cgacgcgctg ggcaatctcc
    12421 acgacgggtt cgatgtcggc cgggagcgtc cgagccgcga acccgggctg gagggagagc
    12481 cgcgcctcgg tcacgaggcg cgcgagcgcc tcgccctccg cgcgggccgt gggccccgcg
    12541 gccgggatgc ccaggaccgc cgtgtcgacg ccgaggcgct ccatgagaca cacgagctcg
    12601 aagcgctggg cgacggtcgg ggccaccgcg gacggcgact gaaggccgtc ccgcagggtc
    12661 tcgtcgacga gctcgacggg ggcatggcgc ggccgcccct cgccggcggc gcgattccag
    12721 tcgtaaacga ggcggctgcg gtccatcggg cctttcgttg ccgggaagct gttcccccgg
    12781 cgggtgcacc cggcttacac tggggattat ggattcgatc gggattgtcg gctacgactc
    12841 gttccacttc gtcgtcacgg accttgcccg gagccgccgc ttctacacgg aggcgctggg
    12901 gttcacggaa gtggcccgcg cgggccgcga aaaggtggag cgcgggggcg aggaagcctc
    12961 cgtcttcggc gcgggccaga tccgcgtcct cgtctcgacg cccgtgcgca aggattcgcg
    13021 cgcggcgcgc tatctgcgaa tccatccgga cggcatctcc tcgctcgcat tccgcgtgaa
    13081 ggacgtcgag gccgcctgga agttcctcga cgcgcgcggg gccacgttcc tgtcggacat
    13141 ccagacggac gagacgccga acggggcgtt ccggacgttc aacatcgcga cacccctcga
    13201 cgacgtcacc ttccgcttca tccagcggga cggggactac gctcatttcg cccccgggtt
    13261 cgagaccgtc ggcgacggca agccgaagga gaatccgcac ggtttcgcga cgatcgacca
    13321 cgtcacgtcg aacgcctaca cgatgctccc cgtcgtgaac tggtaccgcg acgtcctcgg
    13381 cttcacgcac tactgggacg tcgagttcca caccgccgag gtcgcgtcgg cgcggccgat
    13441 ggccggctcg ggcctgcgct ccatcgtgat ggcggacccg ggcagcgggg tgaaattcgc
    13501 gacgaacgag cccatgcgcc cgttcttccg ggagagccag atcaaccgct tctgcgagga
    13561 ccaccacggc gccggcgtcc agcacgtcgc gttcctcgtc aaggagatca ttccggccgt
    13621 cgaggcgctg cgtgcgaagg gcatcgcctt cctcccggcg ccgggcgcgt actacgaccg
    13681 tctgccgaag cgcatggccg aggtgaagat ctcgaagctc cgcgagccca tggagctcct
    13741 ccgcaagaac cacatcctcg tcgacggcgc cgacgagaag tacatgctcc agatcttcat
    13801 gctcgacgcg atggcgatgt acggcgacga gacggccggg ccgttcttct acgagctcat
    13861 ccagcgggaa ggggaccgcg gcttcggcta cgggaacttc cgcgcgctgt tcgagtcgat
    13921 cgagtcggcc cagcggtccg gctcgtcggc gctcgaggtc gtcggttgat cgactggatg
    13981 cggtgcgggg agctgcccgc caagccgcac accgcgttcc gctcgcccga aggggccctc
    14041 cgcttcgagg agtgcctcac gcggcggggt ttcgacggcg agttctcgat cctctaccac
    14101 gacgggccgc cgatgaccga ctccctcatc gggcccgcga cgcccgggcg ctgggaccgg
    14161 aaggcgccga tcccggccga gcaccagccg ctccggcgcc ggctccttct tggcgaaaag
    14221 gctccggagg gtttcacgcc cgtccttttc aacgaggacg tggcggtctc tctgttccgg
    14281 ctgccgcggg aggccgagtc accagacggg ttttcgaacg gcgacgggga cgatcttttt
    14341 ttcttcttag aaggtgaagg aaccctcgag actccgttcg gcgacgtcgc gtttcgggcg
    14401 aacgactacc tgctcgtacc gcgcggggtc gtcgcgcgct tccgcatttc ttcacccctc
    14461 gaaggaattc tcttcgagct ccgcggcggt ttgcatctgc ctcgcgagtt ccggaacccc
    14521 gtcggtcagc tccggatgga cgcgccgttc acgcaccgcg atttcgtgcg gcccgagttc
    14581 aaggggccgc gctccggccc gaagcgcatc ctcgtcaaga aagacgacgc cttcgtcgag
    14641 cggttccccg tcgcgtcgcc gttcgacgtc gtcggctggg acggcaccgt gtggccgttc
    14701 gcgttcgaca tcctgaagta ccagccgagg accgggctca tccacctgcc gcccacgatc
    14761 cattcgacgt tcgcggggcg caacgcgctc gtctgttcgt tcgtaccgcg ggtgacggac
    14821 acgcatcccg acgcgatccc ctgcccgtac ccgcacacgt ccgtcgactg cgacgagatc
    14881 atctactacg tccgcggcaa tttcacgagc cgcaagggcg tcggcccgaa ggccgtctcg
    14941 ctccaccccg ccggagtccc gcacggcccg catcccggcg cctacgaagg gtcgatcggc
    15001 acgcgctcga cggacgagct cgcggtcatg atggacacct tcgcgcccct ccgcctcacc
    15061 gagcaggcgc tcgccctcga gtcgcccgga taccacgaca gctggatccc gggctgaccg
    15121 agggcgggcg gaattccccg ccccgggcgt gaaggttttc ggccacacct gctccaatga
    15181 gacaggatgg gagtcgagat gagccgggaa tcctgcgaag gcttcgagat cgcgatcgag
    15241 atgcggctcc acggagccgg agaagccgaa gcacttgccc ggctggaccg gcacctcgag
    15301 ggctgcgcca gctgccggga attcgaccgg ctgggctccg agtccgaggg ccacctccgg
    15361 gcggacgccg aaggcgcctt ggccgcggcg aacctgaagc gtttgagagc gcgaggcgtg
    15421 ggcctggccc tgtcgcgctg gaacgacgtc ggagcgttct ttctgttcat cgggtttgcg
    15481 tttctcttga ggtccagcct gagggcggcc gggttggttc cgggggaagc ctcccttatg
    15541 gattggagcc tcgccttgat gtggatcgcg attccattcg ttgagattcg ccgacgccgg
    15601 agggccatcg aggaagcctt tcgctcggac gtcctgcttt tcgagatgcg ctcagacgtc
    15661 caccagcgcc tcttcacggc cggagcggag gatgccgcgc aggagacgta cctctcggtc
    15721 acgaaggctc tgaggagctt ccgcggcgac tcgagcgtct cgacctgggt ctaccgcatt
    15781 gcgattcggg cggctcttcg cgtcaaagcg cagcttcgag caccgcaccg tcctcgccct
    15841 cttctccctc gagggcctct cccaccgcca aatcgccgaa acgctcggga tccccgaggg
    15901 aacggtcccg gctccacctc gcccggaaga agctcgcggc cgagctggac gcgctgggct
    15961 tcgagcgagc ctgaaactcg cgggaagcgg cttcgtatca cctctgatgc ctgaacacgg
    16021 aggaacgatg aagccacgcc tcgcccttgc cgcactcgcc ctcgcggcgg cgatcgggcc
    16081 ggtcgccccc gccgcggagc cgcccgcccc cgcccgcgcc ccgatccccg tcgactaccg
    16141 gaagctcccg aacggcctca aggtggtcct gtcgccggac cacacctccc ccatcgcggt
    16201 cgtcgccgtc tactacaaca tcgggttccg gatcgagccg cgcgaccgga ccggtttcgc
    16261 ccacctcttc gagcacctca tgttccaggg ctcgaagaac ctcgggaaga tggagttcat
    16321 caagctcgtc caggcgaacg gcggcatcct gaacggctcg acgcgcttcg acttcacgaa
    16381 ctacttcgag gtcgtgcccg cgaacgcgct caagaccgcg ctctgggccg aggccgaccg
    16441 catgcgcggc ctcgcgatca cgcaggagaa cctcgcgaac cagcagggcg tcgtgaagaa
    16501 cgaggtcaag gtcaacgtcc tgaaccggcc gtacggcggc ttcccgtggc tggacatgcc
    16561 acaggtcgcg aacgagaact ggttcaacgc ccacaacttc tacggcgacc tcaaggacct
    16621 cgacgccgcg actctcaagg acgccgaggc gttcttcaag gctttctact cgccgggcaa
    16681 cgccgcgctc gtcgtcacgg gcgacttcga gaccgaggcg accttcaagt ggatccagga
    16741 gtacttcggc ccgatcccgt cggccccgca ccaggagctc cccgacatct ccgagccccg
    16801 gcaggagaag gagaagcgga cctccaagca ggacgcgcag gcgccgcgcc ccgcgctcgc
    16861 cgtcgcgtac cacgcaccga agcgcaacac gcccgagtac tacgccctcg gcctcctcga
    16921 gcagatcctc gcgggcggca gcgacagccg cctctacgac gctctcgtgc gcaggggcgc
    16981 gctgaccggc gaggtgaact cctcgatcaa cggactcgga aaccagttca acatcgccgg
    17041 cccgacgctc ttcacgctct cgctgttcca cgaccgcgac aagactcccg acgcgatcct
    17101 caaggtcctc gacgccgaga tcgagaagct ccggacgacg ccggtcgacg ccgcgacgct
    17161 cgagcgggcc caggtcaaga tgaggtccga cctctacgcg atgcaggaga gcacgttcgg
    17221 cttcggccgc gcggatctcc tcgcctcgtt cgcgctcttc gacgacgacc cgggacggat
    17281 caacggcctc gagaaggagt tcgcgaaggt cacgcccgag ctgatccgga agaccgcaga
    17341 ggaatacctc cgccccacga accgcacgat cctcgcgatc gagccgaagc cggaggcccc
    17401 caagctcccc ggcccgaagg tctgaggaag gacgccatga cgattcgcaa gtcattcacg
    17461 ctcgtgttcg ccgctgcgtt cctcgcgctc gcgcccggcc tgcgcgccga ccagcccgcc
    17521 gtccgcgaga agcctccaga acccgacgcg ccgcgcccgt tcaagatccc ggcgccgaca
    17581 cgcttttcgc tcccgaacgg cctgaaggtc tcgctcgtcc cttacgggac ggtcccgaag
    17641 gcgctcgtgc gcctcacggt ccacgtcggc aatgccgacg accccgcagg ccagacgtgg
    17701 atggccgacc tcacgggcga cctcctcctc gaaggcacga agacgcgcag cgcgaccagg
    17761 atcgcgcagg acgccgcctc gatgggcggc gaagtcgccg tgcaggccgg cgagaacctc
    17821 acgaccgtcg gcggcgaggt cctctctgaa ttcgccccgg ccatggtcga cctcgtcgcc
    17881 gacgtcgttc gcaatcccgc cttccccgag gcggaactcg cgcggctcaa ggcgaacctc
    17941 gagcggcggc tctcgatcgc gaagagccag tcgcagcaga tggccgccga ggcgttccgc
    18001 gccgcgatgt atccgggcca gccttacggc cgcctcttcc ccgccccgga cgcgctcaag
    18061 ggctacacgc tcgcgaacgc gaaggcgttc tgggccgggc acttcggtgc cgaccgctcg
    18121 gcgctctacg tcgtcggcgt gttcgacgcg aaggccaccg aggcggcgat ccgcaaggcg
    18181 ttcgacggct ggggcaaggc cgccgccgct gcggccccgg cgccgaagcc gtccgcgaag
    18241 cgcacgctca ccgtgctcga ccgcccgggc gcttcccagt ccaccgtcat cctcgggatg
    18301 cccgtcgtcg acccgtcaag ccccgactac gtcccgctcg tcgtgacgaa cgcgctgctc
    18361 ggcggctcgt tcggctcgcg catcacgagc aacatccgcg agcagaaggg ctacacgtac
    18421 tcgcccttca gccaggtctc cgtccggcgc cagggggcct actgggccga gaccgcggac
    18481 gtcacgacga acgtcacggg cgcctcgatc aaggagatct tcggcgagat cgaccgcctg
    18541 cgcgccgagc cgccctccgc ggccgagctc aagggaatcc agaactacct cgccgggacg
    18601 ttcgtcctcc agaactcgtc gcgcatcggc atcgcgaacc agctccagtt cctcgcgctt
    18661 cacggcctgc cggactcgta cctgtccggg ttcgtctcgc gcgtccacgc ggtcacgccc
    18721 gacgaagtga cccggatcgc gaaggcgtac atccggcccg agcaaacgac gctcgtcgtc
    18781 gtcggcgacc gcaagacgat cgacgaccag ctcaagccgt acgcgcctcc cgcgcagccc
    18841 tgaccgagca cgcccgggat tcctcagcgg ccggcgaact tcgccggccg cttctcgagg
    18901 aacgcggaga cgccttcctt catgtcctcg gtcgccgcgc agagcccgaa gagcgtcgcc
    18961 tcgtgctcct cggcggcggc gaacggcatg tcgaggcccg cgttcacggc gtcgaggacg
    19021 tagcgcaacg cgaccggcgc cttgccgagg atcttcttca gcgtgtcgcg gcaggccggc
    19081 aggagctcgg caggctcgac gaccctgttg acgaggccga tccggaacgc ctcgtcggcc
    19141 gggatcgcgt cgcccgtgag caggagctcg agcgctcggc cctttccgac gagccggggc
    19201 aggcgctgcg agccgccgta gcccgggatg agcccgaggt tcacctccgg ctgcccgagc
    19261 ttcgcgttct tcgacgcgta gcggaacgtc gcagccatcg cgagctcgca gccgcctccg
    19321 agcgcgaagc cgttcacggc ggcgaccacg ggcttgccga ggttctcgat caggtcgaac
    19381 gtcgcctgcc ccttgcgcac gaagtcgcgc gcgccgacgg gcgtgaaggc cttgaactcc
    19441 gcgatgtcgg ctcccgcgac gaatgccttc tctcccgcgc cggtcaggat gacggctccg
    19501 acgtccgggt tctccttcgc cacggcgaac gcggcgcgga gcgcatcgag gacctcgccg
    19561 ttcagggcgt tcagcttctc ggggcggttg accgtgacgg tgaggatgcg ctcggaaacc
    19621 tctgtgagga cgacgctcat ggcctcaccc gaagggattc tcgtccgcgc tgggcccgta
    19681 gatggacggc agcgggacgt cgaggagccg caggtaaacg gagagctgcc cgcggtggtg
    19741 gatgaggtgg ttcaggagga tcgtccgcac gaacgcgatg cgcggcatct ccatgatgac
    19801 tttcgtgccg tgccggaggg tccagggacc catcgcgcgc acgtcgtcga gctgcgccat
    19861 cgccgccttt gcgcctgccg cggactcctc gaacgtcttc atgagcgcct cggcgctcgc
    19921 cggctccttc gccgtgccgt cgcctcccgt ggccaggtcg aactcacccg gcagcagcga
    19981 gcccgcgatc cagtgcggca gccccgcgat gtgccccgca agggcgccga gcgacatcga
    20041 cttcgtgtgc ggcttccagg agtacttgtc tgccggcacc cgggacagga gcttgcgggt
    20101 cgtcgagctc tcgcggtcga actccatcag catcggatcg atcatgcgca tggctcagcc
    20161 ctttcgattt cccttttcgt cgtattcgaa gaatccccgc cccgtcttcc tcccgaacct
    20221 cccggcgagc acgaggcgct tgaggagcgc gggcggcgcg aagcgcgtct cacgatactc
    20281 cgcgaacatg atctcggcga tcgcgtagac cgtttcgagc ccgacgtaat ccaggagctc
    20341 gaacgggccc atcgggtggg cgcagccgag cttcatcccc gtgtcgatgt cctcgatcga
    20401 accgaccccc tgctcgtgcg cccggatcgc gtcgaggagg aaaggcacga ggagccggtt
    20461 cacgatgaag cccgaattgt cgcggcaggc gaccggctcc ttgccgagcg cacgggcgaa
    20521 cgccgtgacg gccgcgaccg tcgcgtcgga cgtcgcgagg ccgcgcacga cttcggcgag
    20581 cttcatgacc ggcaccgggt tgaagaagtg cagccccgcg aaacgatccg gcctcttcgt
    20641 cgcggccgcc atctcggtga tcgtcagcga cgacgtgttc gagcagaaga tcgtttcggg
    20701 cccgcaaagc gcgtcgaggg cctggtagac ctcgcgcttc acgtcgaggt tctcgacgat
    20761 cgcctcgacg atgagatccg aagaagagag ttcctcgagt gaggaggaga aagtcagacg
    20821 cgaggcaatc gcgatttcct ccgcatcgtt cagcttgccg cgttgctttg cctttgaaag
    20881 tgaattcgtc acccgcatgc gccccttcgc gagcagctcc tcggtgggtt cgcggacgac
    20941 gaccgtgaac cccgccctcg cgcacacctc ggcgatgccc gaccccatca gcccggcccc
    21001 caggattccg accttgcgaa tttcgctcat gacaatgcct ccacgacgac cgcgattccc
    21061 tggcccccgc cgatgcaggc cgaggcgagt ccccagcgct ggccgcggcg gcgcagctcc
    21121 ttgaggagcg tgagcgtgag gcgggcgccg ctcgccccga gcgggtgtcc gagcgcgatc
    21181 gcgcctccgt tcacgttcag cttcgcccgg tcgacgccga gctccttcat caccccgaga
    21241 atctggcccg caaacgcctc gttgatctcg acgaggtcca tgtccgcgag cgacttcccc
    21301 gctttcgcga gcgccgcgcg actcgccgcg accgggccga ggcccatgaa cgcgggatcg
    21361 caccccgtga ccgcctcggc aaccagccgg ccgaggggca cgatgccttt cgcgcgggcg
    21421 cgctcctccg tcgtcacgac catcatcgcg gcgccgtcca cgatgccgga cgcgttgccg
    21481 cccgtcacca tgccgtcctt gccgaacgcc gggctcagct tcgcgaggcc ttccagcgtc
    21541 gtgtccggcg tcatgtggtc gtccttcgag accgtcacgg tcttgcggcc cttcttcgcc
    21601 tccaccggga cgacctcctc cgcgagacgc ccggacgccc acgcgtccgc ggccttcttc
    21661 tggctctcga gggcgaacgc gtcctgctcc tcgcgcgtga tcccgtggcg gcgggcgacg
    21721 ttgtcggacg tctgggccat cgcgagaccc ggcaccgtgt cgaagaggcc gctcatgagc
    21781 gagtcctcga gcttgccctg cccgagccgc agcccggtgc gcgcgccgcg gaccacgaac
    21841 ggcgcctggc tcatgttctc cgtcccgccc gcgaggacgg cctccgcctc gccgaggagg
    21901 atccggtgct tcgcctggac gagcgcctcg aaccccgagc cgcagaggcg gttcacggtg
    21961 acggccggaa cctcctgcgg cacgccggcg cgcagcccga cgtggcgcga aaggtagatc
    22021 gcgtcggacg acgtctggag gacgttgccc acgatcacgt ggccgatctc gcccggcgca
    22081 aagcgcgcgc ggcggagcgc ttcctgcgcg gccacgacgg cgaggtccgt ggccgtcatg
    22141 tccgcgaaga ggccgttgta ctcggccatg ggcgtgcgtg cgccctcggc gacgacgatg
    22201 gaaggtgagg ccatgcggcc ctccgggcgg gggcgagtcg aacgctcgcc gttcgccggc
    22261 cgatcttatc gagtcccgcc ggatgagacc ggatcgcccc ggcgggccgt cagagtccgg
    22321 ccgcgatctc gtggaaacgg cgccggaagg cgttcatctc cgcggcgcgg gcgtccgggt
    22381 gaagacgatt cgtgaggagg accgccacgc gcccgagccc gggagcgacg aagagcgacg
    22441 cacccgtgaa gcccgtatgg ccgtaggcgg acgggcccag cggctcgccg gcgctccccg
    22501 gcgtcgacgc gagctgccag ccgaggccgc ggtcctcccc gaggcccgcg gtcgcgttct
    22561 ccgtggcgag agcgaagaaa cgctcgggca ggaggcgtcc gtcgcgcgcg agccacgcac
    22621 ggcccacggc ggccgccgcc tcggccgtcc cgaagagtcc cgcgttgagc gacactccgt
    22681 tgccccgccg gaaggcgttc ccgtcgttga cctgtccgtt cacgacgccc tgccggaacc
    22741 cgccgtactt gagcttgcgg tcggcggtca tcgcgcgctc ggtcgcgtcg tcccgctcgc
    22801 ctcctgccgt gcgcgcgaga tcttcggggg aagagggaga gaagagaaga ttctttgaaa
    22861 ggtgaagagg ggcggagact cgcgcctcga aaagcctgtc gagcgttccc ccagagactc
    22921 tttcgacgat ctctgaaagg aagagatagc ccgggcagga atagatcacc ttcgtcccgg
    22981 ggatcgccgc ggcgtccaac tcgcccagcg tccgggcgta cgcggcgcgc ccctctcccc
    23041 tcgagtaaac cggaaaccag tccacgattc cgccgagatg cgcgagcaag cgcctgacgc
    23101 tgacaccttc taagaagctc ttcctccacc gatcctcgaa tcgccacacg gtgtcgtcga
    23161 gcgagagggc gttctctccc gccaaagaca aaaccagcgc cgcccccgcg agaggcttcg
    23221 tcagcgacgc gagatcccag agcgtgtcgg gccccgccgc gcggcgctcg ctcgccggct
    23281 cgacgcgcgc gtcgcccgcg tgccgttccg cgagcgtctc cttttccgag accacgagcg
    23341 ccgaggcgcc cgcgaagaac ccgcgctcga gtccctcgtc gagaagcgag ggaaggctgt
    23401 ccggagcgct cacggctttc cggtcggggc cttggagggc tccttcgcgg cggacggcag
    23461 cagcgggcga tccagcgcct cgtgagcggc cgcgaggcgc gcgcggaacg gctcgagcgt
    23521 cttcatgttg gcccggaccg aatccggctc cttttcgcgg gactctcgcc gcacgcgctc
    23581 ggcctccgcc tcgaagttcg cgatctgctc gttgatgagc ttccgagagc cttcgtccgc
    23641 cgcgtccgcc ttgcgcgccc tcaggtcggc gagggtcttg tcgagcatca cgagtgcgga
    23701 ggcgttgagg cgcaccgtca tcgcggcggc ctccgcctcg agcacgcgct ccttcaccca
    23761 gaggtactct tccgtgttga cgttccgggc gcgtgccacg gtgacgtccg ccgactcgga
    23821 cgcgagcgga tcggacggcg gcggcgcctt ccccgccttc cggtcgagcc gcacgcggtc
    23881 gagcacgccg aggtacgtct cgacctgctt cggagtcacc cggccgtcca cgggcgggcg
    23941 gtagacgtcc ctcgagacgc cggggcgcga gaccccggct gcgggcgtcg gggccgtcgg
    24001 cgcgtccgat ccgccgcagc cggagaggac cgcaatcgcg aaaaacggcg gcagaaacgc
    24061 gactcggcgc ccgaacacgc ccgaatgata gccgcgtggt acgatttttt tcttcaacat
    24121 gctgacccag gtcgggaaat acctgatcga ggagaaggtc ggggtgggcg gtttcggaac
    24181 cgtctacaag gggcgggatc cgttcatcaa gcgcgcggtc gcgatcaaga cctgccagag
    24241 cgacgaagag gagatcaaga agcgcttctt ccgcgaggcg gagttcgccg ggaacctgca
    24301 ccaccggaac atcacgacga tctacgactt cggcgtcacc gacgacggta cgccgtacat
    24361 cgtccaggag ttcctgaccg gagaggacct cgacaagctc gcgcgcaagc gcagtccgct
    24421 gcccctccgg cgcaagctcg agatcctcgt ggacgtctgc gaggggctcg ggtacgccca
    24481 cacggccggg atcatccacc gcgacatcaa gccgtcgaac gtccgcatcc tcgaggacgg
    24541 gacggtcaag ctcatggact tcgggatcgc gaagtccatg gtctcgcagt ccacgctcac
    24601 gcagaccggc atcacgctcg ggaccgcgtc gtacctcgcc ccggagcaga tccgcggcga
    24661 ggatctcgac gcccgcacgg acctcttcag cctcggcgtc ctcgcgtacg agctcaccac
    24721 ggggcagaag cccttcacgg gcgaccacat ctcgacggtg ctctatcgga tcatgaacga
    24781 gcagcccgcc ccgccctcga ccctcgttcc cgggctgccg agggccctcg atgcgatcct
    24841 cgtgaagctc ctcgagaagg atcgcacccg ccgccacgcg aactgcgccg aggtgaaggc
    24901 cgagctctcc gcggttctcg cgggcctctc ggatgctcct gagcacgatg cgcccgccga
    24961 ggacatggac cggacggtcg cgacgccgtc cgccggatac cagaccggtg tggccgcgag
    25021 gccggcgcgc gtgctcgaca ccccgctttc cccctcggcg cccctccgcc agacgcaggg
    25081 cggggccgcc gacgtccggc tgcgcgctac cggcggcgcc ccgatccttc ccccacccgc
    25141 cgccgaagcg cacgcgcggg agagcggccc ggtgctgcgc gtcctgctct cggccgcggt
    25201 cgtcgttctc gcgggaggcg gaggcttctg gctctacgtc cagaagaagt cggcgaagcc
    25261 gcagccgccc gcttccgccg acgccgcggc tccggccgct accgcggccg cgacgaccgc
    25321 cccgtccacc ggatccacgg cg
//
LOCUS       DAOMFY010000028        24227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_669_length_24227_cov_7.273622, whole genome shotgun sequence.
ACCESSION   DAOMFY010000028 DAOMFY010000000
VERSION     DAOMFY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24227
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_669_length_24227_cov_7.273622"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="PLB01_04260"
     CDS             complement(<1..1912)
                     /locus_tag="PLB01_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQQ76546.1"
                     /translation="MNRVPNGRKGRRVLAAAALASLALFGAVRADAERVVDDRTFRVL
                     DGPLGPDVRITTPPVTLDDGSSIEFEVSQLQVFAPGAQIVVHGRFGDTLAPAPTDRWF
                     TGRVVGDPSSVVVLARGLTLRGFVVTGGRVSVLGPERHPYGDGPPGRTFVRTLDPETD
                     VPDAMRTFTCGTDTLPVPPETKPSPSLNRHALSSVMYYAGIAVETDYELYAKFNSTVN
                     LTKYVGDLFAFVSAVYQRDVLVTLQVNYLSIWTTAADPWNATSGTSAALDEFLAYWNA
                     NRTAVPRSTAHMLSGKGLGGGIAYLSVLCNGSYGYGVSASLSGVAPTNISYTYWDFMV
                     VAHELGHNFSSPHTHCYTPPVDQCYAGQSGCYSGTTSVPPEKGTIMSYCHLLSGGYSN
                     IKMFLGVPGETSQAVTAQIRTYVEGRPSCFGTVAGPVVTGIVPASGPAAGGTAVTISG
                     TGFVSGATVMIGGVAATGVAFVNATTLNATTGAHADGVVDVTVKNPSNQGYTATSIYT
                     YGTGIPPTPTPTPTATRTPTPTATRTPTPTITPTLTPTATATPTQTPTFQPGTPTPTP
                     TLTPTPTRTFTATATATRTSTRTPTPTIPPTPPPPTPTLTPTQTPTPTRTPTPGATTF
                     FTLAPCRLVDTRG"
     gene            complement(1954..2820)
                     /locus_tag="PLB01_04265"
     CDS             complement(1954..2820)
                     /locus_tag="PLB01_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76547.1"
                     /translation="MILVALGVLSGLASAQSPQPAPTAADPARILKARNAMRPLGREA
                     RLGPWRLLTDAAMGELTGMDAVAERLASTYTPRYGLAAAPAPGQVVVIYSSDDRYRRF
                     VESDGAPSFAARGHAGAGLAVFALDITPVRSRARLVHELTHLLSSAAVGTPLPPWLDE
                     GLAEDLAWCRVDAEGRLQPDTFDSREIPDAWIQSARAGKSLPLAALLAPDTRFFANPG
                     TRRDATATSAMLVRWCLGDAVRAAAFRAFLKSVAKGGAGDAAALAAALGTGAPEMQKD
                     FLAWGRQFPSGP"
     gene            3085..3678
                     /locus_tag="PLB01_04270"
     CDS             3085..3678
                     /locus_tag="PLB01_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ76548.1"
                     /translation="MQPAIAGGGSLSEAAVLRAVLSDELPASDDVASEAIGTTPGEWD
                     WNALNNVLRPKLVSLAMRKYRFSREEAEDAVQTVYMRVLARDPRVKDPIGYVKIAFLN
                     TCLNIATSRERRTEQMPEDMDVADPSCEKVTARLEAVRMVNKAIRSAGPRCKKIVRLY
                     FGQEYSLAEMTKATGYSQKTVWKRIWGCLDKMRKVLA"
     gene            3675..5111
                     /locus_tag="PLB01_04275"
     CDS             3675..5111
                     /locus_tag="PLB01_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76549.1"
                     /translation="MSAEFNALAACPEHWQLFELITEGSTELNAYVKQHVETGCPVCN
                     GRLEDIQTIERARVMKDEFDAFFDELPSDDAPADEREKFLSQFKRKNRPADRLGRLSF
                     DGKQIAAEIVKVAGEQGIEAAKSRIDAIEADPSRGYALLYAAQLAMPKVSSDPAMYVE
                     LAKAIAAASKSLPFLKDKGPAQPVCREQVMGEAQLLESNALNFLGLPAKAREAARNAR
                     KSLVNAGEDSFALALADYYEGSAANFEGDNKSAWKLLRSSLDEFRLYGQENWCGKAEA
                     SLGTLLVTRNRNQSALYFFEGALTALHPEQDAVAYGITLANRAYCLLQLGQLEAARAT
                     YAKALGFARRLDLRTLVHGIRYGLASIEIKNGQILKALNSFSKIAEDAHEEGLEAHVL
                     SAKLRAAECLGRLGKREEMLTRVRALRREIPEFSVEFDAALRELFAQADEADISSELV
                     AHVVEYLEARERGVQKGYKPFRLVVNGN"
     gene            5300..5431
                     /locus_tag="PLB01_04280"
     CDS             5300..5431
                     /locus_tag="PLB01_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76550.1"
                     /translation="MDYMFFEFLKFFFGGDSPEDASAGVSPIGAGVSPIGAGVSPIG"
     gene            complement(5524..5895)
                     /locus_tag="PLB01_04285"
     CDS             complement(5524..5895)
                     /locus_tag="PLB01_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76551.1"
                     /translation="MRTTGADHQAMLEPADPVVLLPPDVDGSSHRLRAASVLPVLDQR
                     KTSHLVIWEAVEPEGPMGFEPDQRVFVCAYEAAGGAPFMTAEISPGVEAEELTSPHDS
                     GAFAIGLEKDSGEWSIRRLGL"
     gene            complement(5904..6227)
                     /locus_tag="PLB01_04290"
     CDS             complement(5904..6227)
                     /locus_tag="PLB01_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76552.1"
                     /translation="MKSLVSMTSKVLGIAIAITILGAGPANGEYFQGCMRCGPTGCVN
                     VADQRFGGEGCQSYYGMNAGNFGIVCNESPSICYRDSISITVTGSSLDNNINCDIWSW
                     LMGWC"
     gene            complement(6224..8605)
                     /locus_tag="PLB01_04295"
     CDS             complement(6224..8605)
                     /locus_tag="PLB01_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76553.1"
                     /translation="MRPALLLTTFALGRALSAEEVVLDVVNGYTKTLPRQVRYVTGDA
                     QPQKAASKAADLQGGKVTLVVEGKPSILTVTADGFEPFKLDLRRRPSSMGLRPLASIR
                     PTLPSAAGAILGPGSSVRVFISNSHDPRPREVAPVTVVDQGVSFQAEAGTWRGALVVD
                     GRHVAPFNFSAAPGESRTVDTLLPFGLKTRLLAPVKEDGSPAEGVVARWHFATAVSDT
                     QTAQGNRAAAGAEPGLTRLCEELLDMGTKRSPSNAVLRVDAPFGGLLKASLSLESPGL
                     RRLSVGPQDIAANDTGQAARLVMRALPTMRVTVPKLRPGDDSPLTATISATAPSNDGA
                     TAGAKAKANASVVPGSSFSIRALADTDHRIEVRDGQGRMQGLARIARSDRDWPQDVIN
                     RTLRREERTISGTVLWGDAPLRGARVVALYLNEGPSVKPGEPSLDNLSLPFDKVFEVG
                     DETSADGRFSLTVFVPGVYSVMAYHPELKYAKGGPPVDLSEVNRGETEIRIRDAYIVL
                     EVRDDESGEPVKGALAVIRNKPKGGETAGKGTYRFKSGVDGQIRVADLLAGQLDIQIT
                     ATGYRSERVDAAPASTTPIEPRRVNLQRAPSCSIRFLDEAGGPLAGAQAYAVGAEASQ
                     TIFPSYQDLGLTDDSGFLSMDSPSGGFPHLLALATGKKLKFVPSFFTVGSCAQADVFF
                     EGRRASILPQVRSSEGKALAGVQIAFATGGVFLPWNLAAMIGSLDAAMPARFMTTGSA
                     GEIQLAAALEDGQWQAFAFKVDGGQGVELQKRIGSFELPLSRSTTIVVTGGKK"
     gene            8676..9341
                     /locus_tag="PLB01_04300"
     CDS             8676..9341
                     /locus_tag="PLB01_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ76554.1"
                     /translation="MTPARLCSTWTRRLVMPRFGIPPSAVEIAGLTPNGNGNFAPLER
                     HCAEAQSSRVSPAAGRPSSGFEVAEFLVLDRKLVRKLVALARSRFGIDETDAEDLVQE
                     TALEIARSPALIASPEDYAFQVFHNRCSRFLERRVRRNAMFVQPRDWDATPAADEKLD
                     EKAIVRAGFARISATCRGLLTSYYVEGASLKETAARSGHSPKQVWKRLNACLKKLKET
                     IGA"
     gene            9287..11575
                     /locus_tag="PLB01_04305"
     CDS             9287..11575
                     /locus_tag="PLB01_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76555.1"
                     /translation="MEAAERLPEEIEGDDRSMSATHPDDTTLLRSIHGDLPDRSQAAL
                     QKHLARCRPCRDQRDGTKRLHERLAGAGDSLAFHVGDLFAARPVPPQAEAPRGAALKA
                     MATALGRLDEAKSVLKAAIEEDGSRAGLGLMLDDAAARLAAVHLLEEAATGDPSEKLA
                     AFAAAVARRIEEAAPEGEEEVDAVVSRAQLAAVAGLVTGNWRLFCGRPKEAAGSFAAA
                     WKALAGFDAPEHLVAWVEAGESLRRSYEGRPVEGRLLAERALETFERYGLARGALRAR
                     HARAVALYTASQFREAHREFRAALGSKVATQLDRARAVSGAAFCLAARGRFHEAAKEY
                     SRVRRRLKGEGAQAEQYLLQGEMKVALGTAGRLHQRGDGLAAFALPEVGGAFHARVIA
                     AEIVKLAGEQGIEAAKARIQAIEADPSRGYALLYAAQLAMPKVSSDPAMYVELAKAIA
                     AASKSLPFLKDKGPAQPVCREQVVGEAQLLESNALNFQGMPSEARAIARAARESFVQA
                     GEDSFALALVDYYEGSAASFEMDERDAWTLLRSAHSEFQLFGQENWIGRAEAALGTLL
                     VRRGKNAAAIRFLDGALTNLHPEQDGSAYAQLLVNRGYALVQLGNLDAAKATYAKALG
                     FARRLDLKVAVFIVRYGLASIELKNGQIFKAVHSFSRIAEDAQAEGLLQRVLSAQLRV
                     AECLGRLGKREEMLTRIRALPREIPGVSVEGDPAIRELFAQADEADVSSELVAHVVEY
                     LEARDRGVQKGYKPFRLVGNAH"
     gene            complement(11583..11870)
                     /locus_tag="PLB01_04310"
     CDS             complement(11583..11870)
                     /locus_tag="PLB01_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76556.1"
                     /translation="MSPESVLGTITGYCLSIFGAGAFVRTARATGSPDDLRAIRRRLI
                     LIGFLTFAAAALFLRQPTLQDWAQFGFLFSAIANSAFLLAMFLRLRVLSRV"
     gene            complement(11875..12258)
                     /locus_tag="PLB01_04315"
     CDS             complement(11875..12258)
                     /locus_tag="PLB01_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HQQ76557.1"
                     /translation="MPLVRISLREGKAPEYRRAVADSVHDAMVATIGIPKDDRFQVIT
                     EHGEGGLIYDPGYLGIKRSDDVVFIQITLSTGRTVEIKKALYARIAENLSKSPGLRKE
                     DVFVNLVEVVKENWSFGNGDAQYSG"
     gene            complement(12311..12736)
                     /locus_tag="PLB01_04320"
     CDS             complement(12311..12736)
                     /locus_tag="PLB01_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQQ76558.1"
                     /translation="MTASLKFSMVPGTYAVCRLPASAAVPAWAGKAFVSITRTAEELS
                     IVCEERRLPEDVFGIEFQIERGWVLLKLHGPFPLDAIGVLSSVAKPLAEAAISLFALS
                     TFDTDYILVKRIHAKQAIAALTLAGHTLVEPTSGAFRRS"
     gene            complement(12786..13472)
                     /gene="phoU"
                     /locus_tag="PLB01_04325"
     CDS             complement(12786..13472)
                     /gene="phoU"
                     /locus_tag="PLB01_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQQ76559.1"
                     /translation="MKEHFSERLETLRRQLIAMGAEAERQIASAIEALVTGDAAKARA
                     VIEGDKVVDQMEIKIDEGAVELLATQQPVAGDLRLLVSALKINADLERIGDHAVNIAE
                     AAERMSKSRPFKPWIDIPYMAEIARGMLQDSLDAFVRRDAALALAVCKRDDLLDEKNK
                     SIIRELLTYMAENPTLITACIEIIGISRNLERVGDLATNIGEETIYIAQGDVIKHHMG
                     QRPSEPAPDA"
     gene            complement(13469..15295)
                     /locus_tag="PLB01_04330"
     CDS             complement(13469..15295)
                     /locus_tag="PLB01_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ76560.1"
                     /translation="MSRFFRRAALPALAGLVLGVLFALVLGRVLDGAVRERGRSELAL
                     KLDRLVPEFEADLKSRTAPIERVRLAARQAGARLTLVEGDGSVLADSEVAPEQIGAVE
                     NHGSRPEIVAARRDLVGFGERRSATVLEPFVYVARRVGEEANPLGYIRLAISQNDLDS
                     LEAPFRRTLIQISVLAGLLFGLVITFIRQRHARELERVRAGVAEAAAGRQPAPPEGGS
                     EETQEVYVALARFARLVSAERAGSEKARLLARTVFEQVPVGLLVVDRSLALLDANPAA
                     LALFHVPAGAPRAALVDLVRHPDALHLFEAGVAASSAEAAPSSAVLRFGGELTPEQIL
                     EITVRSVPHRDAHGEPAAIGVVRDVTEREQMETMRRRFVSDVSHELRTPIAAIRAAVE
                     TLAGEDELPADIARLLGILERQSSEMEALVSDLTDLSQIESGAVTLQPERISVRALLT
                     SVVRDLQSAAEARNVTVAVDTPERLAVSGDRRRLAQVFRNLVDNAVKFSPAGGRVDVL
                     AEGGAPGPDGLAPCVVHVVDRGIGIPRTEQARIFQRFYRVDPSRAKSVPGTGLGLAIV
                     KHILILHGGSIRVESEPGKGSRFTVSLPATTPREAPAPEETK"
     gene            complement(15292..15990)
                     /locus_tag="PLB01_04335"
     CDS             complement(15292..15990)
                     /locus_tag="PLB01_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ76561.1"
                     /translation="MKPAPGPARHRIAVVEDDDDLAFTLTYNLKKDGRYEVERYAGGL
                     VAMDELLVRPPDLVVLDLNLPDVDGLTICRELKKHEETRNIPILMLTARAEERDKLLG
                     FELGADDYVTKPFSMKELLARIAAHLRRSASPESPDDVFEDGRLKIDRARHTVDVRGK
                     QVHLTKKEFDLLWLLIRSGGRVVSRETILSRLWDYAADIETRTVDVHIRSLRKKIGED
                     VIETVVGVGYKFSK"
     gene            complement(15987..16814)
                     /gene="pstB"
                     /locus_tag="PLB01_04340"
     CDS             complement(15987..16814)
                     /gene="pstB"
                     /locus_tag="PLB01_04340"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQQ76562.1"
                     /translation="MTSSAPGHEAQAPIVSAPDRSVEGRPDVITCRNVNLFYGEKQAL
                     FDITIGCKKNAVTAFIGPSGCGKSTLLRCLNRMNDLIDGVKITGTIAIHGEDIYKRGT
                     DVTAIRKRFGMVFQKSNPFPKTIFENVVYGLRIQGENRRSVLEETAERSLRNAALWDE
                     VKDRLDDSALGLSGGQMQRICIARAIAVEPEILLMDEPCSALDPIATSKVEELIFELK
                     ERYTIVIVTHNMQQAARVSDETGFFMLGKLIEFDKTETIFTKPRQKTTEDYITGRFG"
     gene            complement(16841..18460)
                     /gene="pstA"
                     /locus_tag="PLB01_04345"
     CDS             complement(16841..18460)
                     /gene="pstA"
                     /locus_tag="PLB01_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQQ76563.1"
                     /translation="MSARDFFFKKGEPMIWLAGSGLAVSLLLIFGLLTLVAVRGLGFF
                     WPQPLVLIETADGKRVLGEILTREPVPEAKDGGQRIRVKIANRDLNGLDFRWIEEKEI
                     ARRETPPNALLLERQEYGNFHGFLKELRRGDEAIGGADAAGLARLEAEQARIRPAFEA
                     LRQTEKKEVGAINAALERDRLAVRRAEMKKDPDLEKLHAASAARQAELKRRYEDLRER
                     LEAQRRELSKVVATFVDANGKSKVVPVRDLVRIVAPNGASVWTKAGVYLSRLGEFVLG
                     EPRESNTEGGVFPAIFGTVLMVVLMTAVSVPFGVVAALYLREYAKQGRFVRLVRIAVN
                     NLAGVPSIVFGIFGLGFFVYGIGGRIDQLFFAEALPTPTFGTGGIIWCALTLAILTVP
                     VVVVATEEALAAVPAGMRHASLALGATKWQTTWRVVLPAAAPGILTGLILAMARGTGE
                     VAPLMIVGVVKLAPTLPLDGVFPYLHLERKIMHLGFHLYDVGFQSPNVEAAIPMVYTT
                     TILLLAIVVALNLTAIVIRNSLRKKLKTSTF"
     gene            complement(18460..20658)
                     /locus_tag="PLB01_04350"
     CDS             complement(18460..20658)
                     /locus_tag="PLB01_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ76564.1"
                     /translation="MSNRTAASRRFWTRASDRIATAAISGGGLLMIGSLVLIFILIVS
                     ETLPLFAPPKSALLRTVPVSGAAPLAATADEYQEAFALLFPDGTVRALDASGALVKEL
                     PVPGLAGAAVTAAARSIKGELALGLANGTAVVATPAWETAFEGEKRRRSVSLSALPPL
                     ALDPAGRALTRIAFVARDGSALVAATASPGTLFVGVASSEAADAFARDLSASAAGEEI
                     TALSFLDGGAVLCAGTASGKVLTFPLEDPKSPGAPVSTAVHAGSPSPITALETLLGGQ
                     TLVAGDAKGRVTGLLRVRTDSAAEARTLNAVKEFEPQGAAITAIAGSPRGKTFVTGDA
                     GGGLVARFGTNTRTLVKTRASGPVAAIGVAPKADGYVAVTPKGVEDFSLEAPHPEISI
                     GALFGKVLYEGYDKPEYVWQSSGGTDDFEPKLSLVPLVFGTLKGTFYAMLFAVPIALA
                     AALYTSVFATPRVKRFVKPIVEIMAALPSVVLGFLAGLWLAPLLQKATLSALLMVPMI
                     PLFAVLGVLAFQMLPFDVRQRLHPGQELLLVLPLAVLATGFAAVAGPWVERIVFAGDF
                     KAWLLASASLKYDARSSLVIAFAMGFAVIPIIFTISEDALSSVPEHLKAASLALGASP
                     WQTAVRVILPTASAGIFSALMVGFGRAVGETMIVLMATGNTPVLEWNIFNGMRTLAAN
                     IAVEIPEAPQGGSLYRVLFLAALLLFLVTFLVNTLAEVLRQRLRKKYTVI"
     gene            complement(20722..21693)
                     /locus_tag="PLB01_04355"
     CDS             complement(20722..21693)
                     /locus_tag="PLB01_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQQ76565.1"
                     /translation="MNTLKKAAAGALALALSATVLAETVKVDAAIPAYKRASGVSGNL
                     NSIGSDTLNNLMTYWAEGFKKAYPNVNIQIEGKGSSTAPPALTEGTAQLGPMSREMTG
                     AEKDKFEAKYGYKPTQIRVALDGIAIYVNKDNPIKQLTFKQIDGIFSSTRKRGGKDIK
                     TWGDLGLTGEWAAKPISIFGRNSASGTYGYFKEHALAKGDFKNSVKEQPGSSSVVQGV
                     AEDKYAIGYSGVGYTTSGVKTVPLSEGDAGPFYGESYENVLSRKYPLSRYLYVYTNKA
                     PGKALDPLVKEFAGFVLSKEGQEIVVKDGFFPLTSAVVAEELAKLAK"
     gene            21938..23320
                     /locus_tag="PLB01_04360"
     CDS             21938..23320
                     /locus_tag="PLB01_04360"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQQ76566.1"
                     /translation="MNRWINRFAALALVGAVLLVAAAPAAAQGLWYKEVEKDGRIYVF
                     NTSAGFQSWEKSGEIGNKAVTMIGNGPKGETVVAENETAIDMYNFKHDRPGYNRPVPK
                     PAVSAPPTVLRWGTEGELKFGLLLQGWYITDDSPYATNNATSSTLGNFIGSNTFRLRR
                     SEIKFNGKVNKDWAFEVMLDPAKAIVSSGAVTNNNTTDAKILQDLAVTYTGFKGWELS
                     LGQKKITLTEEGVRSSSELDFPERSQIVRAVSDRRETGFFVKGDVAEPVTLWGSISNG
                     TASNTLDDSNDTISYCGRVDLKFVKGLNFGGSGCKSAGETAAHTTRSRVGGHARFNGG
                     AALPLVAEAEYYYAQDGQAAAPGVLRRSGWYAQALYTVMDRFQLGGRYDIITTNLDAQ
                     DGTNGVTQKDSQIKTWTFGIHWLPLGHDKYKNVSLKLDYFIVQQDNRLVNGVLSDSYN
                     QFLLAAQVAF"
     gene            23425..24201
                     /locus_tag="PLB01_04365"
     CDS             23425..24201
                     /locus_tag="PLB01_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HQQ76567.1"
                     /translation="MGSLHSFSAVAFVENLSLRDAAAAFPGSALTPHDLRVPSAGGGD
                     VFVYPFGAVVFRDVPAAARDAFLETLRKAFPALTGEVVREEFGVLEEAGSVTGFHEGR
                     LFVDALTPERSSVVSLTVAQSAAMEYYEGIVEALARRTQRIVDRLQARGTVSFWLRPL
                     HRFVGEAVSTRTEVLSVLHLFDRPDATWEDPALDRIYADLRAEFDLLDRFDALERKLR
                     SVQEALELVLDVARDRRLTTLELIIVLLIVVEVVQAFLKR"
BASE COUNT         3912 a         7826 c         8591 g         3898 t
ORIGIN      
        1 cgccacgcgt gtccacgagc cggcagggcg cgagggtgaa gaacgtcgtg gcccccgggg
       61 tgggcgtacg cgtgggcgtg ggcgtctgcg tcggggtcag cgtgggagtc ggcgggggcg
      121 gtgtcggcgg aatcgtgggc gtcggagtcc gcgtcgacgt gcgcgtcgcc gtcgccgtgg
      181 ccgtgaacgt gcgggtgggc gtcggcgtca acgtcggcgt gggcgtgggg gtgccgggct
      241 ggaacgtcgg cgtctgcgtc ggggtcgcgg tggcggtcgg cgtcagggtc ggcgtgatcg
      301 tcggcgtcgg cgtccgggtc gccgtcggtg tcggcgtccg ggtcgccgtc ggtgtcggcg
      361 tcggggtcgg cggaatgccg gtcccgtacg tgtagatcga cgtcgccgtg tatccctggt
      421 tggaggggtt cttcacggtc acgtcgacga cgccgtccgc gtgtgctccc gtcgtcgcgt
      481 tgagtgtcgt cgcgttcacg aacgcgacgc ccgtggcggc cacgccgccg atcatcacgg
      541 tcgcccccga gacgaagccc gtgcccgaga tcgtgaccgc ggtgccgccg gcggccgggc
      601 cggaggccgg gacgatgccc gtgacgacgg ggcccgcgac ggtcccgaag cagctcggcc
      661 ggccctcgac gtaagtccgg atctgcgccg tgacggcctg gctcgtctcg ccgggcacgc
      721 cgaggaacat cttgatgttc gagtagccgc ccgagagcag gtggcagtag ctcatgatcg
      781 tccccttctc gggggggacg ctcgtcgtgc cgctgtagca gccgctctgg ccggcatagc
      841 actggtccac gggcggcgtg tagcagtgcg tgtgcgggga gctgaagttg tggccgagct
      901 cgtgggcgac gaccatgaag tcccagtacg tgtacgagat gttcgtgggc gcgacgccgg
      961 agaggctcgc actgacgccg tagccgtacg agccgttgca caagacgctc aggtacgcga
     1021 tgccgccgcc gagccccttc ccggaaagca tgtgcgccgt cgaccgggga acggccgtgc
     1081 gattcgcgtt ccagtaggcc aggaactcgt cgagcgcggc ggacgtgccg ctcgtcgcgt
     1141 tccacgggtc ggcggccgtc gtccagatgg agagatagtt gacctggagc gtcacgagga
     1201 cgtcgcgctg gtagaccgcc gagacgaagg cgaataggtc gccgacgtac ttcgtgagat
     1261 tcaccgtcga attgaacttc gcatagagct cgtagtccgt ctcgacggcg atccccgcgt
     1321 agtacatgac gctgctcagc gcgtgccggt tgagcgaggg cgagggtttg gtctcggggg
     1381 gaacgggcag agtgtccgtc ccgcacgtga aggtccgcat cgcgtcgggg acgtccgtct
     1441 cgggatccag cgtccggacg aaggtcctgc cgggagggcc gtcgccgtaa gggtggcgct
     1501 ccggcccgag aaccgagacc cgcccgccgg tgaccacgaa gccgcggagc gtgagcccgc
     1561 gcgcgaggac gacgacggac gacgggtcgc cgacgacgcg ccccgtgaac catcggtcgg
     1621 taggtgccgg ggcgagggtg tcgccgaagc ggccgtgcac gacgatctgc gcgccgggcg
     1681 cgaagacctg gagctgggaa acctcgaact cgatactcga gccgtcatcg agcgtgacgg
     1741 gcggggtcgt gatccggacg tccggcccga gcggcccgtc gaggacccgg aacgtcctgt
     1801 cgtccacgac ccgctcggcg tcggcccgga cggcgccgaa gagggcgagc gacgccagag
     1861 cggcggccgc gaggacacgg cgtccttttc gcccgttcgg aacccggttc atggacacac
     1921 ttcctgcaag gaagtgttgc aggcccacgc gggtcatggg ccggaaggga attgccgccc
     1981 ccacgcgagg aagtccttct gcatctccgg cgctcccgtc ccgagcgccg ccgcgagagc
     2041 ggccgcgtct cccgcgccgc ccttcgcgac ggacttcaga aacgcccgga acgccgcggc
     2101 ccgcacggcg tcgccgaggc accagcgcac gagcatggcg ctcgtcgccg tcgcgtcgcg
     2161 gcgcgtgccc gggttcgcga agaaccgcgt gtcgggggcg aggagcgccg cgagcggaag
     2221 cgacttcccg gcccgggcgc tctggatcca cgcgtcgggg atctcgcgcg aatcgaacgt
     2281 gtccggctgg agccggccct ctgcgtccac gcggcaccac gcgaggtcct cggcgagccc
     2341 ttcatcgagc cagggaggga gcggcgtccc cacggcggca gaagagagaa ggtgcgtgag
     2401 ctcgtggacg aggcgggcgc gcgatcggac cggggtgatg tcgagtgcga acacggcgag
     2461 cccggcgccc gcatggcccc ttgccgcgaa cgagggagcc ccgtccgact cgacgaacct
     2521 cctgtaacgg tcgtcgctgg aatagattac gacgacctgt cccggcgccg gggcggcggc
     2581 gaggccgtat cggggggtgt aggttgacgc gaggcgctcg gccacggcgt ccatgcccgt
     2641 cagctctccc atggccgcgt ctgtgaggag ccgccagggc ccgaggcggg cctcccggcc
     2701 aagcgggcgc atcgcattgc gcgccttgag gatacgggcg ggatcggcgg cggtgggcgc
     2761 aggctgaggc gactgggcgg aggcaaggcc cgacaggacg ccaagggcga cgaggatcaa
     2821 gagcctcgaa tgagtaaaga agcctcgccg catcttcagc gaggttaccg gatcttcgcg
     2881 gcaagggcgg aagaagagaa ataccagcga atctgaactc tatttattaa ctattctaag
     2941 aaaatcttcc tcttcgattt ccctcttgac acgccccggt ttatgaaaca attccgcaag
     3001 gattaacccg gagcacccgc ggggtagcgg gcgaagctcc tttttggaga ggttctcatg
     3061 gctgcatggg accccacaga cggaatgcag cccgcaatcg cgggcggtgg aagtctgtcc
     3121 gaggccgcag tcctgcgcgc ggtcctctcc gacgagctcc cggcgagtga cgatgtcgcg
     3181 tccgaggcca tcggcacgac gcccggcgag tgggactgga acgcgctcaa caacgtcctg
     3241 cgcccgaagc tcgtgagcct cgcgatgcgg aagtaccgct tcagccgcga ggaagcggaa
     3301 gacgcggtgc agacggtcta catgcgcgtc ctcgcgcgcg acccgcgcgt gaaggacccg
     3361 atcggctacg tgaagatcgc gttcctcaac acgtgcctca acatcgcaac gtcgcgcgag
     3421 cggcgcacgg agcagatgcc cgaggacatg gacgtcgccg atccgtcttg cgagaaggtc
     3481 acggcgcgcc tcgaggccgt gcgcatggtc aacaaggcca tccgcagcgc gggcccgcgg
     3541 tgcaagaaga tcgtccgcct ctacttcggc caggagtaca gcctcgccga gatgacgaag
     3601 gcgacgggct actcgcagaa gaccgtgtgg aagcgcatct ggggctgcct cgacaagatg
     3661 cggaaggtgc tcgcgtgagc gcggaattca acgccctcgc cgcctgcccc gagcactggc
     3721 agctcttcga gctgatcacc gagggctcga cggagctgaa cgcgtacgtg aagcagcacg
     3781 tcgagacggg ctgcccggtc tgcaacggca ggctcgagga catccagacg atcgagcgcg
     3841 cccgcgtcat gaaggacgag ttcgacgcgt tcttcgatga gctgccgtcc gacgacgcgc
     3901 cggccgacga gcgcgagaag ttcctgtcgc agttcaagcg gaagaaccgc cccgccgacc
     3961 gcctcgggcg cctgtcgttc gacggcaagc agatcgcggc cgagatcgtc aaggtcgcgg
     4021 gcgagcaggg gatcgaggcg gcgaagtcgc gcatcgacgc gatcgaggcc gaccccagcc
     4081 gcggctacgc gctgctgtac gcggcgcagc ttgcgatgcc gaaggtctcg agcgacccgg
     4141 cgatgtacgt cgagctcgcg aaggcgatcg cggcggcgtc gaagagcctc ccgttcctga
     4201 aggacaaagg ccccgcgcag cccgtctgcc gcgagcaggt catgggcgag gcgcagctgc
     4261 tggagtcgaa cgcactgaac ttcctcgggc tgccggcaaa ggcgcgcgag gcggctcgaa
     4321 atgctcgcaa gtcgctcgtt aatgcggggg aggattcctt cgccctcgcg ctggcggact
     4381 actacgaggg ttcggccgcg aacttcgagg gcgacaacaa gtccgcgtgg aagctcctga
     4441 ggtcgtccct tgacgaattc aggctctacg gccaggagaa ttggtgtggg aaggccgagg
     4501 cgtcgctcgg cactctgctc gtcactcgca acaggaacca gtcggccctc tatttcttcg
     4561 agggagccct cacggctctc catccggagc aggatgccgt cgcgtacggc atcacactgg
     4621 cgaatcgtgc gtattgcctc ctgcagctgg gccagctcga ggcggcaagg gccacttacg
     4681 cgaaggctct cgggttcgct cggcggctcg acctgaggac gcttgtccac gggatccgtt
     4741 atgggctcgc ttcgatcgaa atcaagaacg gccagattct caaggcgttg aattctttct
     4801 cgaagatcgc agaggatgcc cacgaagaag ggctcgaagc gcacgtgttg tccgcaaaac
     4861 tgagggcggc ggagtgcctt ggacgcctcg ggaagcgcga ggaaatgctc acgcgtgttc
     4921 gggctcttcg ccgcgagatc ccggaattct cagttgagtt cgacgcggcg cttcgcgagc
     4981 tattcgccca ggccgacgag gccgacattt ccagcgaact ggtcgctcac gttgtcgagt
     5041 acctcgaggc ccgagagcgc ggcgttcaga aggggtacaa gcctttccgt ctggtggtca
     5101 acggaaactg atctttctct agacaacgtt caatagtcag ggggcggggt gccatgaatc
     5161 gggtgcctca aaataaatct ttcaagaggg gggtcaaagg tgtgcccgcc cctgtccgta
     5221 tatatagccc acggttagat agcccagtat caaccaacag ttggccgccc gatggggtcg
     5281 ggtgggccgg gaggacgtca tggattacat gttcttcgaa tttctaaagt tcttctttgg
     5341 cggtgattcg ccggaggacg ccagcgcggg cgtctcgccg attggcgcag gcgtctcgcc
     5401 gattggcgca ggagtttcac ccatcgggtg aatttcctaa cccactttcg cccaatttga
     5461 ctggctaggc cagccttgtg ataggtgttt tcgcgcgtgc aaacgcgcga tttggtgctt
     5521 tatctagagc ccgagcctgc gaatcgacca ttcccccgag tccttctcca ggccgattgc
     5581 gaacgccccg ctgtcgtgcg gggaggtgag ttcctcggcc tcgacccccg gtgagatctc
     5641 ggccgtcatg aacggcgccc cgcccgccgc ctcgtaggcg cagacgaaga cgcgctgatc
     5701 cggctcgaac cccatcgggc cttccggctc caccgcctcc cagatgacga ggtgcgaggt
     5761 cttcctctgg tcgaggacgg ggaggacgga cgccgctcgc aggcggtggc tggagccgtc
     5821 gacatcagga ggcaggagaa cgacgggatc ggcgggctcg agcatggcct ggtggtccgc
     5881 tcccgtcgtt ctcatatccc ggcctagcac cagcccatca gccaggacca gatatcgcag
     5941 ttgatgttgt tgtcgaggct gcttcccgtc accgtgatcg aaatgctgtc gcggtagcag
     6001 atgctggggc tctcgttgca cacgatgccg aaattgccgg cgttcattcc gtagtaggac
     6061 tggcaccctt ccccgccaaa tctctggtct gcgacgttga cgcatcccgt cgggccgcac
     6121 cgcatgcagc cttgaaaata ttcgccgttc gccgggcctg cgcccaggat cgtgatggcg
     6181 atggcgatgc cgaggacctt cgaagtcatg gaaacgagcg atttcatttc ttccctcctg
     6241 ttacgacgat cgttgtggat ctactgagcg gcagttcgaa agagccgatc ctcttctgca
     6301 gctcgacgcc ttgtcccccg tctaccttga aggcgaacgc ctgccactgc ccgtcctcca
     6361 gcgcggcggc cagctggatc tcgccggccg atccggtggt catgaagcgg gccggcatcg
     6421 cggcgtccag ggagccgatc atcgcggcga gattccatgg gaggaacacg cctccggtcg
     6481 cgaaggcgat ctggacgccc gcgagggctt ttccctccga gctgcgcacc tgaggaagga
     6541 tcgatgctcg ccggccctcg aagaagacgt ctgcctgggc gcagctgccg accgtgaaga
     6601 aagacggtac gaacttcagt ttcttcccgg tggcgagcgc gagcaggtga ggaaatcctc
     6661 cggagggaga gtccatagac aagaaaccac tgtcgtcggt taagccgagg tcctgatacg
     6721 acgggaagat ggtctggctc gcctcggcac ccactgcata ggcctgagcg ccagccaagg
     6781 ggcctcccgc ttcatccaga aaccgaatcg agcagctcgg tgctctctgg agattcacgc
     6841 gccggggctc gatcggtgtc gtcgaggccg gagccgcatc gactcgctcg cttcgatagc
     6901 cggtggccgt aatctgaatg tcgagttgcc cggccaggag gtccgccacg cggatttgac
     6961 cgtcgacgcc tgatttgaag cggtacgtgc ccttgccagc ggtctcgccc cccttgggct
     7021 tgttccgtat gacggccagg gctcccttca cgggttcccc cgactcgtcg tcccggactt
     7081 cgaggacgat gtacgcgtct cgaatcctga tctccgtctc gcctcgattc acctcgctga
     7141 gatcgacggg aggtccgcct ttcgcgtact tgagttcggg gtgataggcc atcacgctgt
     7201 agacgcccgg cacgaagacc gtcaaggaga accggccgtc ggcgctcgtt tcgtcgccga
     7261 cttcgaacac cttgtcgaat ggcagcgaga ggttgtcgag tgaaggctcg ccgggcttca
     7321 cagacggccc ctcgttcagg tagagcgcaa cgactcgtgc gccccggagg ggggcgtcac
     7381 cccagaggac ggtcccgctg atcgtgcgct cctcacgtcg aagagtccgg ttgatcacgt
     7441 cttgcggcca gtcgcgatcc gacctcgcga tgcgagcgag cccctgcatc cgcccctggc
     7501 catctctgac ctcgatcctg tggtctgtat ctgccagagc tcggatcgag aaggaacttc
     7561 ccgggacgac gctagcgttg gccttcgcct tcgctccggc tgtggctccg tcgttcgacg
     7621 gtgcagtcgc ggagatcgtg gccgtgaggg gcgaatcgtc ccctggtctt agtttgggaa
     7681 ccgtgaccct cattgtcggg agtgctctca tgacgagccg tgcggcctgg ccggtgtcgt
     7741 tggcggcgat gtcttgaggg ccaacggaga gtcggcgcaa gcccggtgac tcgagggaca
     7801 gggaggcctt gagcaagccg ccgaagggcg cgtcgacgcg aagaaccgcg ttgctcgggc
     7861 tgcgcttcgt gcccatgtcg aggagttcct cgcacaggcg cgtgaggccg ggttcggcgc
     7921 cggcggccgc ccggtttcct tgcgctgttt gcgtgtcgct gacggctgtt gcgaagtgcc
     7981 agcgtgcgac gaccccttcc gcgggtgagc cgtcttcctt gacgggcgcc aggagccgcg
     8041 ttttcagccc gaacggcaag agcgtgtcga ccgttcggga ctcgcccggg gcggcggaga
     8101 agttgaaggg agcgacgtgc ctgccgtcaa cgacgagcgc accgcgccat gtgccggcct
     8161 ccgcttggaa cgacacaccc tggtcgacga ccgtgaccgg cgcgacttca cgaggcctcg
     8221 gatcgtggga gttcgagatg aacactcgaa cggaagagcc cggcccgagt atcgccccgg
     8281 cggcggatgg caacgtgggt cgaatcgagg cgagagggcg cagccccatg gacgagggac
     8341 gtcggcgcaa gtccagtttg aacggctcga agccgtccgc cgtgaccgtg aggattgacg
     8401 gctttccttc tacgacgagc gtcactttgc cgccctgaag atccgccgcc ttcgaggcgg
     8461 ctttctgggg ttgcgcgtcg cccgtaacgt acctgacctg ccgaggcagc gtcttcgtgt
     8521 atccgttgac gacgtcgagg acgacctcct cggcgctgag ggcgcgcccg agcgcgaagg
     8581 tcgtcagaag aagcgcgggt ctcatgcctc gatacatgct tcgatcttgc tccgaatggg
     8641 tctttcgtcc atagagtgga tccctgccgg agggattgac cccggcccgc ctgtgttcga
     8701 cttggacgag gcggctggtg atgccccggt tcggcattcc tccgagtgcc gtcgagatcg
     8761 ccggattgac ccccaacggc aatgggaatt tcgcgcccct tgaacggcat tgcgccgaag
     8821 cgcagagttc gcgcgtgagt cctgccgcgg gccgaccgtc ctccggtttc gaggtcgccg
     8881 agttcctcgt gctcgaccgg aagctcgtgc gcaagctcgt cgccctcgcg cggtcgcgtt
     8941 tcggcatcga cgagaccgac gccgaggatc tcgtgcagga aaccgcgctc gaaattgcgc
     9001 gcagcccggc gctcatcgcc tcgcccgagg actatgcgtt ccaggtgttc cacaatcgct
     9061 gctcgaggtt cctcgaaaga cgagtgagac ggaacgcaat gttcgtgcag ccgcgcgatt
     9121 gggacgccac gcccgccgct gacgagaagc ttgacgagaa ggcgatcgtc cgggccggct
     9181 ttgcacgcat ctcggcgacg tgccgggggc tcctgacgag ttactacgtc gagggcgcga
     9241 gcctgaagga gacggccgcg cggagcggac attcgccgaa gcaggtgtgg aagcggctga
     9301 acgcctgcct gaagaaattg aaggagacga tcggagcatg agcgccaccc accccgacga
     9361 caccacgctc ctgcgctcga tccacggcga cctgcccgac cgctcgcagg cggctctcca
     9421 gaagcatctc gcgcgctgcc ggccgtgccg cgaccagcgc gacggcacga aacgcctgca
     9481 cgagcggctc gcgggcgcgg gcgactctct cgcgttccac gtcggagacc tgtttgcggc
     9541 ccggcccgtc ccgccgcagg ctgaggcacc gagaggagcg gcactgaagg cgatggcgac
     9601 cgcgctcggg cgtctcgatg aagcgaagtc cgttctgaag gcggcgatcg aggaggatgg
     9661 ttcgagagcg gggctgggcc tgatgctcga cgacgcggcg gctcgcctgg ctgccgtgca
     9721 ccttctcgaa gaggcggcga cgggagatcc ctcggagaag ctcgcggcct tcgcagcagc
     9781 agtcgctcgt cggatcgagg aagctgcgcc cgaaggcgaa gaggaagtcg acgcggtggt
     9841 ttcgcgagcg cagctcgccg ccgtggccgg cctcgtgacc ggcaactggc gcctcttctg
     9901 cgggcgcccg aaggaagcgg cgggaagttt cgccgcggcc tggaaggcgc ttgccgggtt
     9961 cgacgcgccc gagcacctcg tcgcttgggt ggaggctggg gagtcgctgc ggaggtcgta
    10021 cgaggggcgt cccgtcgagg gccgccttct ggccgagcgc gcgctcgaaa cgttcgagcg
    10081 ctacgggctt gcccggggcg ccctgcgcgc gcggcacgcg cgcgcggtgg cgctctacac
    10141 ggcgagccag ttccgggaag cgcatcgcga attccgggcc gcgttgggat cgaaggttgc
    10201 gacgcaactg gaccgtgcgc gggcagtatc cggggccgcc ttctgcctgg cggcgcgcgg
    10261 gcggttccac gaggccgcga aagagtactc gcgcgttcgc aggcgactga agggcgaggg
    10321 cgctcaggcg gagcagtacc tgttgcaggg cgagatgaag gtcgcgctgg gaacggccgg
    10381 tcgcttgcat cagcgcggag acgggctggc ggcgttcgca ctaccggagg tggggggcgc
    10441 gttccacgca cgtgtgatcg cagccgagat cgtgaagctc gcgggcgagc aggggatcga
    10501 agcggcgaag gcgcggatcc aggcgatcga ggccgacccc agccgcggct acgccctgct
    10561 gtacgcggcg cagctcgcga tgccgaaggt ctcgagcgac ccggcgatgt acgtcgagct
    10621 cgccaaggcg atcgcggcgg cgtcgaagag cctcccgttc ctgaaggaca agggccccgc
    10681 gcagccggtc tgccgcgagc aggtcgtggg cgaggcgcag ctgctcgagt cgaacgcgct
    10741 gaacttccag ggcatgccct cggaagcgcg ggcgattgcg cgtgctgccc gcgagtcgtt
    10801 cgttcaggcg ggcgaggatt catttgctct cgccctcgtg gactactacg agggttccgc
    10861 agcgagcttt gaaatggacg aacgcgacgc gtggacactt ctgcggagcg cgcactccga
    10921 attccagctc ttcggacagg agaactggat cggccgagcc gaagcggcgc tcgggaccct
    10981 gctcgtccgg cgcggaaaga atgcggccgc tatccgcttc ctcgatggcg ccctcacgaa
    11041 ccttcacccc gaacaggatg gatcggcgta cgcccagctc ctcgtcaaca gaggctacgc
    11101 gctcgttcag ctggggaacc tcgatgcggc taaggctacc tacgcgaagg ctctgggctt
    11161 cgcgcgccgg ctcgacctca aggtggccgt tttcatcgtg cgatacggtc tcgcgtcgat
    11221 cgagttgaag aacgggcaga tattcaaggc cgtgcactcc ttctctcgga ttgccgaaga
    11281 cgcccaggcg gaaggcttgc tgcagcgcgt tctttcggcg cagttgagag tcgccgagtg
    11341 tctggggcgc ctcgggaagc gcgaagaaat gctcacccgg atccgggcct tgccgcgtga
    11401 gatcccgggc gtttccgtcg aaggtgatcc ggccattcgc gagctattcg cccaggccga
    11461 cgaagccgac gtttccagcg agctggtcgc tcacgttgtc gagtacctcg aggcccgcga
    11521 tcgcggcgtt cagaaggggt acaagccttt ccgtctggtg ggcaacgccc actgatcgtt
    11581 ttctagaccc ggctgagaac gcgcaggcgc aggaacatcg ccagcaggaa ggccgagttc
    11641 gcgatcgcgg agaagaggaa gccgaactgc gcccagtctt ggagcgtggg ttggcggagg
    11701 aagagggcgg ccgcggcgaa cgtcaggaac ccgatgagga tcaggcgcct ccggatggct
    11761 cgaaggtcgt ccggcgagcc ggtcgcgcga gcggtcctga cgaaagctcc cgcgccgaag
    11821 atcgaaaggc agtagccggt gatcgtgccg aggactgatt ccgggctcaa gttctcaccc
    11881 cgagtactgg gcgtcgccgt tcccgaacga ccagttctcc ttgaccacct cgacgaggtt
    11941 cacgaagacg tcttccttcc ggagccctgg gctcttcgac aggttctccg cgatccgtgc
    12001 gtagagggcc ttcttgatct cgacggtcct gccggtgctg agtgtgatct ggatgaagac
    12061 gacgtcgtcg gagcgcttga tccccagata cccggggtcg tagatcaggc cgccttcgcc
    12121 gtgctcggtg atcacctgga agcggtcgtc cttcgggatc ccgatggtcg cgaccatggc
    12181 gtcgtgcacg gagtcggcca ccgcgcggcg gtattcgggt gccttgcctt cgcggaggga
    12241 gatgcggacg agcggcatgg ggcctcctgt ggcgccggac gtttccgctc cggaccgggt
    12301 cctccgggag ctacgagcgg cgaaaggccc ctgatgtcgg ctcgacgagc gtgtgcccgg
    12361 ccagcgtcag cgccgcgatc gcctgcttgg cgtggatgcg cttgacgagg atgtagtccg
    12421 tgtcgaacgt cgagagcgcg aagaggctga tcgccgcctc cgcgagcggc ttcgcgacgg
    12481 acgagaggac gccgatcgcg tccagcggaa acgggccgtg cagcttcagg agcacccagc
    12541 cgcgctcgat ctggaactcg atcccgaaga cgtcctccgg aagcctccgc tcctcgcaca
    12601 cgatcgacag ctcctcggcg gtccgcgtga tcgaaacgaa cgctttcccg gcccaggcgg
    12661 gaacggccgc ggacgccggc aggcggcaga ccgcgtaggt cccgggcacc atggagaact
    12721 tcaggctggc cgtcacgggg acatcgtagg ccttcgccgg ctccggcgca cccccggaag
    12781 gcggatcagg cgtcgggcgc cggctccgag ggccgctgcc ccatgtggtg tttgatgacg
    12841 tcgccctgcg cgatgtagat cgtctcctcg ccgatgttcg tcgcgaggtc gcccacgcgc
    12901 tcgaggttcc gcgagatgcc gatgatctcg atgcaggccg tgatgagcgt cgggttctcg
    12961 gccatgtacg tgagcagctc gcggatgatc gacttgttct tctcgtcgag gaggtcgtcc
    13021 cgcttgcaga ccgcgagcgc gagggccgcg tcccgccgga cgaacgcgtc cagcgagtcc
    13081 tggagcatgc cgcgcgcgat ctcggccatg taggggatgt cgatccacgg cttgaacggc
    13141 cgcgacttcg acatgcgctc ggccgcctcg gcgatgttca cggcgtggtc gccgatgcgc
    13201 tcgaggtccg cgttgatctt cagcgccgag acgaggaggc ggaggtcgcc cgccacgggc
    13261 tgctgagtcg cgagcagctc gaccgcgccc tcgtcgatct tgatctccat ctggtcgacg
    13321 accttgtcgc cctcgatcac cgcgcgcgcc ttggccgcgt cgcccgtgac gagcgcctcg
    13381 atggccgaag cgatctgccg ctcggcctcg gcgcccatcg cgatgagctg gcggcgcagc
    13441 gtttcgagcc gttcggaaaa gtgctccttc atttcgtctc ctcgggggcg ggggcttcgc
    13501 gcggcgtcgt ggccggcagc gaaacggtga aacggctgcc cttgccgggc tcggactcca
    13561 cgcggatgga cccgccgtgg aggatcagga tgtgcttgac gatcgcgagg ccgaggcccg
    13621 tgccggggac ggacttcgcg cgcgacgggt cgacgcggta gaagcgctgg aagatccggg
    13681 cctgctcggt gcgcgggatg ccgatgcccc ggtccacgac gtggacgacg cagggcgcga
    13741 ggccgtccgg cccgggggcg ccgccctcgg cgagaacgtc cacgcggccg ccggcggggg
    13801 agaacttcac cgcgttgtcg acgaggttcc ggaagacctg cgcgaggcgg cggcggtcgc
    13861 ccgagacggc gagcctctcg ggcgtgtcga cggcaaccgt gacgttgcgg gcctcggccg
    13921 cggactgcag gtcgcggacg acggacgtga gaagggccct gaccgagatg cgctcgggct
    13981 ggagggtcac ggcccccgac tcgatctgcg agaggtcggt caggtcggag acgagggctt
    14041 ccatttcgga cgactgccgc tcgaggatgc cgaggaggcg cgcgatgtcg gcgggcagct
    14101 cgtcctcgcc ggcgagcgtc tcgacggcgg cgcggatggc ggcgatgggc gtccgcagct
    14161 cgtgcgagac gtcggacacg aaccggcgcc gcatcgtctc catctgctcg cgctccgtca
    14221 cgtccctgac gacgccgatc gcggccggct cgccgtgggc gtcgcggtgg gggaccgagc
    14281 ggacggtgat ctcgaggatc tgctcgggcg tcagctcgcc gccgaagcgc aggacggcgg
    14341 aggacggcgc ggcctcggcc gacgacgcgg ccacgcccgc ctcgaagagg tgcagcgcgt
    14401 cggggtggcg gacgagatcg acgagcgccg cgcgcggcgc gcccgcgggc acgtggaaga
    14461 gcgcgagggc ggccgggttc gcgtcgagga gcgcgagaga gcggtccacg acgagaaggc
    14521 cgacggggac ctgctcgaac acggtgcgcg cgaggaggcg cgccttctcg ctgcccgcgc
    14581 gctcggccga gacgagccgg gcgaagcgcg cgagggcgac gtagacctcc tgcgtctcct
    14641 ccgagccgcc ctccggcggc gcgggctgcc tgccggcggc ggcttcggcg acgccggcgc
    14701 gcacgcgctc gagctcgcga gcgtgacgct gccggatgaa cgtgatgacg aggccgaaca
    14761 gtaggccggc caggacggag atctgaatga gcgtccggcg gaacggcgcc tcgagggagt
    14821 cgaggtcgtt ctggctgatc gccagccgga tgtagccgag cgggttcgct tcctcgccga
    14881 cgcgccgcgc gacgtagacg aacggctcga ggacggtcgc cgaccggcgc tcgccgaagc
    14941 cgacgaggtc ccggcgggcc gcgacgatct cggggcgcga cccgtgattc tccacggcgc
    15001 cgatctgctc gggagcgacc tcgctgtcgg cgaggacgga gccgtctccc tccacgagag
    15061 tcaggcgcgc cccggcctgc cgcgcggcga gccggacgcg ctcgatgggg gccgtgcgcg
    15121 atttcaggtc cgcctcgaac tcgggaacga ggcggtccag cttgagggcg agctcgctcc
    15181 gcccgcgctc gcgcacggcg ccgtcgagga cgcgccccag cacgagcgcg aagagcacgc
    15241 ccaggacgag gccggcgagc gcgggcaggg ccgcgcggcg gaagaagcgg ctcatttcga
    15301 gaacttgtac cccacgccga cgacggtctc gatgacgtcc tcgccgatct tcttgcggag
    15361 ggagcggatg tggacgtcca ccgtgcgcgt ctcgatgtca gccgcgtagt cccagaggcg
    15421 cgagaggatc gtctcccgcg acacgacgcg cccgccgctc cggatgagaa gccagaggag
    15481 gtcgaattcc ttcttcgtga ggtggacctg cttcccgcgg acgtccacgg tgtggcgcgc
    15541 gcggtcgatc ttcaggcgtc cgtcctcgaa cacgtcgtcc ggggactcgg gggaagccga
    15601 gcggcgcagg tgcgccgcga tgcgggcgag gagctccttc atcgagaacg gcttcgtcac
    15661 gtagtcgtcc gccccgagct cgaagccgag cagcttgtcg cgctcctcgg cgcgcgccgt
    15721 gagcatgagg atcgggatgt tccgtgtctc ctcgtgcttc ttcagctcgc ggcagatcgt
    15781 gaggccgtcc acgtccggca ggttcaggtc gaggacgacg aggtccggcg ggcggacgag
    15841 aagctcgtcc atcgcgacga gcccccccgc gtagcgctcg acctcgtacc ggccgtcctt
    15901 cttgaggttg tacgtgagcg tgaaggcgag atcgtcgtcg tcctccacga ccgcgatgcg
    15961 gtggcgggcg ggcccgggcg cgggcttcaa ccgaacctcc cggtaatgta gtcctcagtt
    16021 gtcttttggc gcggcttcgt gaagatcgtc tcggtcttgt cgaactcgat gagcttgccg
    16081 agcatgaaga agcccgtctc gtccgagacg cgcgcggcct gctgcatgtt gtgcgtcacg
    16141 atgacgatcg tgtagcgctc cttgagctcg aagatgagct cctcgacctt gctcgtcgcg
    16201 atcgggtcga gggccgagca gggctcgtcc atgaggagga tctcgggctc gaccgcgatt
    16261 gcgcgcgcga tgcagattcg ttgcatctgg ccgccggaga ggccgagcgc cgagtcgtcg
    16321 aggcggtcct tgacctcgtc ccagagggcg gcgttccgca gggaacgctc ggcggtctcc
    16381 tcgaggacgc tcctgcggtt ctcgccctgg atccggaggc cgtagacgac gttctcgaag
    16441 atcgtcttcg gaaacgggtt cgacttctgg aagaccatgc cgaagcgctt gcggatcgcc
    16501 gtgacgtccg tgccgcgctt gtagatgtcc tcgccgtgga tcgcgatcgt cccggtgatc
    16561 ttcacgccgt cgatcaggtc gttcatgcgg ttcaggcagc ggagaagcgt cgacttcccg
    16621 cagcccgagg gcccgatgaa cgccgtcacc gcgttcttct tgcagccgat cgtgatgtcg
    16681 aagagcgcct gcttctcccc gtagaagagg ttcacgttgc ggcacgtgat gacgtccggg
    16741 cggccttcca cggaccgatc cggagcggaa acgatcgggg cctgcgcctc atggcccggg
    16801 gcgcttgacg tcatgagcgt atccattctc tcttctttcc tcagaaggtg gaggtcttca
    16861 gcttcttgcg cagggagttg cggatcacga tcgccgtgag gttcagggcg acgacgatcg
    16921 cgaggagcag gatcgtggtc gtgtagacca tcgggatggc cgcctcgacg ttcgggctct
    16981 ggaagccgac gtcgtagagg tggaagccga ggtgcatgat cttccgctcg aggtggaggt
    17041 acgggaagac gccgtcgagc ggcagcgtcg gcgccagctt gacgacgccg acgatcatga
    17101 gcggcgcgac ctcgccggtg ccgcgcgcca tcgcgaggat gaggccggtc aggatgcccg
    17161 gcgcggcggc gggcagcacg acgcgccagg tcgtctgcca cttcgtggcg ccgagcgcga
    17221 gcgacgcgtg ccgcatgccg gcggggacgg ccgcgagcgc ctcttccgtc gcgacgacga
    17281 cgaccggcac cgtcaggatc gcgagcgtca gcgcgcacca gatgatcccg cccgtgccga
    17341 acgtcggcgt cgggagtgcc tcggcgaaga agagctggtc gatgcggccg ccgatcccgt
    17401 agacgaagaa gccgagcccg aagatgccga agacgatcga cgggacgccc gcgaggttgt
    17461 tcacggcgat ccggacgagc cggacgaacc ggccctgctt cgcgtactcg cggaggtaca
    17521 gcgccgccac gacgccgaac ggaaccgaaa cggcggtcat caggacgacc atgaggacgg
    17581 tcccgaagat ggcggggaag acgccgccct cggtgttcga ctctcgcggc tccccgagga
    17641 cgaactcccc gaggcgcgaa aggtagacgc cggccttcgt ccagaccgac gcgccgttcg
    17701 gggcgacgat ccgcacgagg tcccggaccg ggacgacctt gctcttcccg ttcgcgtcca
    17761 cgaacgtcgc gacgaccttc gagagctccc ggcgctgcgc ttcgaggcgc tcgcgcaggt
    17821 cttcgtagcg gcgcttgagc tcggcctggc gcgccgcgct cgcggcgtgg agcttctcga
    17881 ggtcgggatc cttcttcatc tcggcgcgcc gcacggcgag gcggtcgcgc tcgagggccg
    17941 cgttgatcgc gccgacctcc ttcttctccg tctggcggag agcctcgaag gcgggccgga
    18001 tcctcgcttg ctcggcctcg aggcgggcca ggcccgcggc gtcggcgccg ccgatcgcct
    18061 cgtcgccgcg ccggagctcc ttcaggaatc cgtggaagtt cccgtactcc tggcgctcga
    18121 ggaggagagc gttcgggggc gtctcgcgcc tcgcgatctc cttctcctcg atccagcgga
    18181 agtcgaggcc gttcaggtcg cggttcgcga tcttgacgcg gatgcgctgc ccgccgtcct
    18241 tcgcctcggg caccggctcg cgcgtgagga tctccccgag cacccgcttg ccgtccgcgg
    18301 tctcgatcag gacgagcggc tgcggccaga agaagccgag cccgcgcacg gcgacgagcg
    18361 tgagaagccc gaagatgagg agcagcgaaa cggcgaggcc cgatccggcc agccagatca
    18421 tcggctcgcc cttcttgaag aagaagtcgc gcgccgacat cagatcaccg tgtacttctt
    18481 gcggaggcgc tggcggagca cttccgcgag cgtgttgacg aggaaggtca cgaggaagag
    18541 gaggagggcc gcaaggaaga ggacgcggta gagcgagccg ccctggggcg cctcggggat
    18601 ctcgaccgcg atgttcgcgg cgagcgtgcg catcccgttg aagatgttcc actcgaggac
    18661 gggcgtgttg cccgtcgcca tgaggacgat catcgtctcg cccaccgcgc gcccgaagcc
    18721 gaccatgagg gccgagaaga tgccggccga ggccgtcggg aggatcacgc ggaccgccgt
    18781 ctgccacggc gaggcgccga gggcgagcga cgcggccttc aggtgctccg ggacgctcga
    18841 gagcgcgtct tcggaaatcg tgaagatgat cgggatgacg gcgaagccca tcgcgaacgc
    18901 gatgacgagg gaactgcgcg cgtcgtactt gagggacgcc gaggcgagga gccaggcctt
    18961 gaagtcgccc gcgaagacga ttctctcgac ccaggggccg gcgacggccg cgaaccccgt
    19021 cgcgaggacc gcgagcggca ggacgaggag aagctcctgc ccggggtgca ggcgctggcg
    19081 cacgtcgaac gggagcatct ggaaggcgag gacgccgagc accgcgaaga gcggaatcat
    19141 cgggaccatg agaagggccg agagcgtcgc cttctggaga agcggcgcga gccagagccc
    19201 ggcgaggaat ccgaggacga cggaggggag cgccgccatg atctcgacga tcggcttgac
    19261 gaagcgcttg acgcgcggcg tcgcgaagac gctcgtgtag agcgccgccg cgagcgcgat
    19321 cgggacggcg aacagcatcg catagaacgt ccccttgagc gtgccgaaga cgaggggcac
    19381 gagcgagagc ttgggctcga agtcgtccgt gccgccgctc gactgccaga cgtactcggg
    19441 cttgtcgtaa ccctcgtaga ggaccttccc gaagagcgcg ccgatcgaga tctccgggtg
    19501 cggcgcctcg agcgagaagt cctcgactcc cttcggcgtg acggcgacgt aaccgtcggc
    19561 cttcggcgcg acgccgatgg ccgcgaccgg gccggacgcc cgcgtcttca cgagggtgcg
    19621 cgtgttcgtc ccgaagcgcg cgacgaggcc gcccccggcg tcaccggtca cgaacgtctt
    19681 gccgcgcggg gaacccgcga tcgccgtgat cgcggcgccc tgcggctcga actccttgac
    19741 ggcgttgagc gtccgggcct cggcggccga gtcggttcgc acgcggagca ggcccgtgac
    19801 gcggcccttc gcgtcgccgg cgacgagcgt ctggccgccg agcagcgttt ccagagcggt
    19861 gatgggcgac ggcgagccgg cgtgcacggc cgtcgaaacg ggcgccccgg gcgacttcgg
    19921 gtcctcgagc gggaacgtga ggacctttcc ggatgccgtg ccggcgcaga gaacggcgcc
    19981 gccgtcgagg aacgacaggg ccgtgatctc ctcgcccgcc gcggaggccg aaaggtcgcg
    20041 tgcgaaggcg tcggcggctt cggaggacgc cacgccgacg aaaagcgtgc ccggcgaagc
    20101 cgtcgccgcg acgagggccg agccgtcgcg ggccacgaat gcgatccggg tgagcgcccg
    20161 gcccgccggg tcgagcgcga gaggcggcag ggcggagagg gagacgcttc gccggcgctt
    20221 ctcgccttcg aacgcggtct cccacgcggg cgttgcgacg acggcggttc cgttggcgag
    20281 gccgagggcg agttcgccct tgatcgagcg ggcggcggcc gtgacggccg ctcccgcgag
    20341 gcccgggacg ggcagctcct tcacgagcgc gccggacgcg tccagggccc ggaccgttcc
    20401 gtccggaaag aggagcgcga aggcttcctg gtactcgtcc gccgtggccg ccagcggggc
    20461 cgcaccggag acgggcacgg tgcgcaggag cgcggacttc ggcggcgcga acaggggaag
    20521 ggtttccgag acgatgagga tgaagatgag gacgaggctc ccgatcatga gcaggccgcc
    20581 gcccgagatc gcggcggtcg cgatccggtc gctggcgcgt gtccagaaac gccgggaggc
    20641 agccgtcctg ttgctcatgc gcgcttagtg gaaatggccg cggcgcacgg gaacgccgcg
    20701 gcacgcatca gatcgttccg tttacttcgc gagcttcgcg agctcttcgg cgacgacggc
    20761 ggaggtcagc gggaagaagc cgtccttgac gacgatctcc tggccttcct tcgataggac
    20821 gaacccggcg aactccttga cgagcgggtc gagcgccttg ccgggcgcct tgttcgtgta
    20881 gacgtacagg tagcgcgaga gcgggtactt gcgggagagc acgttctcgt atgactcgcc
    20941 gtagaacggg ccggcgtcgc cctcggagag ggggacggtc ttcacgccgg acgtcgtgta
    21001 gccgacgccg gaatagccga tcgcgtactt gtcctcggcc acgccctgga cgacggacga
    21061 ggaacccggc tgctccttga cgctgttctt gaagtcgccc ttggcgagcg cgtgctcctt
    21121 gaagtagccg tacgtgccgg aggccgagtt gcgcccgaag atcgagatcg gcttggcggc
    21181 ccactcgccg gtgagcccga ggtctcccca ggtcttgatg tccttcccgc cgcgcttgcg
    21241 cgtggaggag aagatgccgt cgatctgctt gaacgtgagc tgcttgatgg ggttgtcctt
    21301 gttcacgtag atcgcgatgc cgtcgagggc gacgcggatc tgcgtcggct tgtagccgta
    21361 cttcgcctcg aacttgtcct tctcggcgcc cgtcatctcg cgggacatcg gcccgagctg
    21421 ggcggtgcct tccgtgagcg ccgggggggc ggtcgaggag cccttgccct cgatctggat
    21481 gttgacgttc gggtaggcct tcttgaagcc ctcggcccag tacgtcatca ggttgttgag
    21541 cgtgtccgag ccgatggagt tcaggttgcc cgaaaccccg gacgcccgct tgtaggcggg
    21601 gatcgccgcg tcgaccttga cggtctcggc cagcacggtg gcggagaggg cgagggcgag
    21661 cgcccccgcg gcggccttct tcagcgtgtt catcaatttc ctccttcgaa gttctcccaa
    21721 gaccggcgga gtcttgccgg aggactcagg gacgatgtgc aaacgatgtt aaatctcttt
    21781 gaaggacgag gggaggctgg ctgcaacccg ctgcctttag gcgtcttcac cttctaagaa
    21841 aaatctttac gcggcctaca tgttgcaacc gcgcctcctt tacgacctcc gccgagagtg
    21901 ccgcgtcgtc agagaaacgc gacaaggagg aacttcgatg aaccgctgga tcaaccgctt
    21961 cgccgcactc gccctcgtgg gcgccgtgct cctggtggcc gcggcgcccg ccgccgccca
    22021 gggcctctgg tacaaggagg tcgagaagga cggccggatc tacgtcttca acacgtccgc
    22081 gggcttccag agctgggaga agagcggcga gatcggcaac aaggccgtca cgatgatcgg
    22141 caacggcccg aagggcgaga ccgtcgtcgc cgagaacgag accgcgatcg acatgtacaa
    22201 cttcaagcac gaccggccgg gttacaaccg cccggttccg aagccggctg tctcggcgcc
    22261 gccgaccgtc ctgcgctggg gcacggaggg cgagctcaag ttcggcctgc tccttcaggg
    22321 gtggtacatc acggacgact caccgtacgc cacgaacaac gcgacgagct cgacgctcgg
    22381 gaacttcatc ggctcgaaca cgttccgcct gcgccggtcc gagatcaagt tcaacggcaa
    22441 ggtgaacaag gactgggcgt tcgaagtcat gctcgacccg gccaaggcga tcgtctcgtc
    22501 gggcgctgtc acgaacaaca acacgaccga cgcgaagatc cttcaggacc tcgcggtcac
    22561 gtacacgggc ttcaagggct gggagctctc gctcggccag aagaagatca cgctcacgga
    22621 agaaggcgtc cgctcctcgt ccgaactgga cttccccgaa cgctcgcaga tcgtccgggc
    22681 cgtctccgac cggcgcgaga cgggcttctt cgtgaagggc gacgtcgccg agcccgtcac
    22741 gctctggggc tcgatctcga acggcacggc gtcgaacacg ctcgacgact cgaacgacac
    22801 gatcagctac tgcggccgag tcgacctcaa gttcgtcaag ggcctcaact tcggcggatc
    22861 gggatgcaag agcgccggcg agaccgcggc gcacacgacc cggtcgcgcg tcggcgggca
    22921 cgcccggttc aatggcggcg cggccctccc gctcgtcgcc gaggccgagt actactacgc
    22981 ccaggacggc caggcggccg cgcccggcgt cctgcgccgc agcggctggt acgcacaggc
    23041 gctctacacg gtcatggacc ggttccagct cggcggccgg tacgacatca tcacgacgaa
    23101 cctcgacgcg caggacggaa cgaacggcgt cacgcagaag gattcccaga tcaagacctg
    23161 gacgttcggc atccattggc tcccgctcgg ccacgacaag tacaagaacg tgagcctgaa
    23221 gctcgactac ttcatcgtcc agcaggacaa ccgcctggtg aacggcgtcc tctcggacag
    23281 ctacaaccag tttctcctcg cggcccaggt cgccttctaa aagccaacac cgcggcgcgc
    23341 ctcgcaaacg cgccgcctcc tccttgaatg cccccgggcg atcccggggg cttttttttg
    23401 ctgtagcctc ggaagggacg cgcgatgggc agcctccact ccttttcggc cgtggcgttc
    23461 gtcgagaatc tgtcgctgcg cgacgccgcg gcggcgttcc cggggagcgc gctcacgccg
    23521 cacgacctga gggttccgtc ggccggcggg ggagacgtgt tcgtctaccc gttcggagcg
    23581 gtcgttttca gggacgtgcc ggccgcggcg cgggacgcgt tcctcgagac gctccggaag
    23641 gccttcccgg ccctcaccgg agaagtcgtg cgcgaggagt tcggcgtgct cgaggaggcg
    23701 ggatcggtca cgggcttcca cgaggggcgg cttttcgtgg acgcgctgac gccggagcgc
    23761 tcgagcgtcg tctccctcac ggtcgcgcag agcgccgcca tggagtacta cgaggggatc
    23821 gtggaagcac tcgcacggcg gacacagcgg atcgtggacc ggctccaggc ccgggggacc
    23881 gtttcgttct ggctccggcc gctccaccgc ttcgtgggcg aggcggtcag cacccgcacc
    23941 gaggtccttt cggtcctcca tctgttcgac cgtccggacg cgacctggga agacccggcg
    24001 ctcgaccgca tctacgccga cctgcgtgcg gagttcgatc tgctcgaccg gttcgacgcg
    24061 ctcgagcgca agcttcggag tgtgcaggaa gcgctcgagc tcgtcctcga cgtcgcgcgg
    24121 gaccgccgcc tgacgacgct tgaattgatc atcgttctgc tcatcgtcgt cgaggtcgtg
    24181 caggcttttc tgaagaggtg aaagccgagg caccgcccac acgcggc
//
LOCUS       DAOMFY010000029        24214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_671_length_24214_cov_9.166853, whole genome shotgun sequence.
ACCESSION   DAOMFY010000029 DAOMFY010000000
VERSION     DAOMFY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24214
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_671_length_24214_cov_9.166853"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PLB01_04370"
     CDS             <1..1407
                     /locus_tag="PLB01_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HQQ76568.1"
                     /translation="ALAAAGAHAEDAPPAARFRLFPDAAAPAEPKPAGGAASGGREKA
                     STRRFWLTAAEIGILEFLPYAQNRFVNDEDYARISVESIRKNFDAGFSFDNDKFTTNQ
                     LGHPIAGGLFFNAARTNGYSFWESAPFVLAGSTFWEMFGETQGPSLNDLVNTTLGGIT
                     LGEASYRLSQMILDDRARGGARIAREALAGLVNPTQLLTRVFTGEAWAVRSGRGEYVE
                     PSRFVTELDAGGRHFVSSTRTNPDQAVLAAAIRYGDPFDRAVSRPFDSFDLGVDMSYP
                     SSAWLTRVEIRGLLGGWDVDPGETGGRHVFGVFMDFDYTNNDTRMFSSQSFRFGLLSL
                     RPLGGGVELRGEALGAVVPLAALKNDYLEESSGLVGRAYDYGPGAGIYTAIRFRRREL
                     DLATLTYSVFWMHTSNGIGRNASLQSFRVEGRIPVAGPFAVGGSWTWGKRITTYDDYA
                     TYRSDATQWRAFASVMFR"
     gene            complement(1418..2017)
                     /gene="clpP"
                     /locus_tag="PLB01_04375"
     CDS             complement(1418..2017)
                     /gene="clpP"
                     /locus_tag="PLB01_04375"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQQ76569.1"
                     /translation="MLIPMVIEQTNRGERAYDIYSRLLKDNVIFLGSAINDDVANLVI
                     AQMLFLEAENPEKDITLYINSPGGSVSAGLAIYDTMQYVKCDVATYCLGQAASMGALL
                     LAGGAKGKRAALPNARVLIHQPHGGAEGQASDIEIQAREMLRTKQRLNEILAHHTGQP
                     VEKISRDTDRDNIFEPDAAKEYGLIDLVIRRREELKVPA"
     gene            complement(2047..3348)
                     /gene="tig"
                     /locus_tag="PLB01_04380"
     CDS             complement(2047..3348)
                     /gene="tig"
                     /locus_tag="PLB01_04380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQQ76570.1"
                     /translation="MLTSFVDESPTKKVLTFEVPAEDVKAATEKTIKVFAKQVRLPGF
                     RPGKAPAHIIRQRFAEEIKGEVLERLIQESLAGALREKNLIPLGPPKIADLKFEFDAP
                     LAFKVDLEVRPAVEPKDYRGLKVPEGSTAVTAEDVDRVLSRIREGHATYDAVEGRPVK
                     DGDHVLVDIKGTFPAGDGEDFNREKVLVEIGGEGTLPELSAHLRNAEAGATVSFQKSF
                     AADVSDSEFAGKTVLYTVHLVAIKTRVLPALDDELARLALTPREGEAPEGASLAMLRE
                     KIQESLAKEKEQTVREQRRRAALDGLLALNAVPAPESMVESEIDSALKEYARHMARQG
                     VDLKDAKIDWNELRKEARPSAERRVKEYLLLDAIGEKESLSVTDTELDAELKRRAQAM
                     GMSFAELKGALAKADRVEGVREELRIDRVVDFLVAEAVVAG"
     gene            complement(3380..3464)
                     /locus_tag="PLB01_04385"
     tRNA            complement(3380..3464)
                     /locus_tag="PLB01_04385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3428..3430),aa:Leu,seq:tag)
     gene            complement(3489..5615)
                     /locus_tag="PLB01_04390"
     CDS             complement(3489..5615)
                     /locus_tag="PLB01_04390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ76571.1"
                     /translation="MSKREIPSRCARPSRPVAPRRPLHLLRNLLLLSLSVLSIPAFAA
                     RPMTIEDNLLTVRVADPQLSPDGKWVAFVRTTTDLAAGKRNADVWLVPADGSAPPRAL
                     TRHEKADNAPRFSPDGRTLAFVSTRSGSPQVWLLDLSGGEPRKLTDLSAGVADPLVFS
                     PDGKKLAFVSDVYPECADEACSKKKREDLEKDPVKVRRLTRLLHRHWDEWRENVRHHV
                     FVADVASGALTDVTPGDFDSPPNHYEDGGIAFSPDGKEIAFVSNRDGADKEAFTTNKD
                     VWTVPAAGGEAVKLTAGNPAADFDPTYTPDGASIVVRAQRRPTFEGDRWYLDVYDRKT
                     KAKRTLFENPDLSVDEFALSKDGKTVYFTAEREGRRDFFEVSLSGGTPREILRGGNIG
                     ALRVGPSRIVFTKNTLTAPSEIFSLPLEERSSSSSSSSSPKALTDENRSWRKDVAMSA
                     PESLSTTGAGGARVQYWLVKPPMFDPAKKYPVVFLIHGGPQGAWEDGWSARWNPTLWA
                     AQGWVVVAPNPRGSTGFGQKFVDEISGDWGGKVMVDLDAVFDAVVKLPFVDASRQGIA
                     GASYGGYAVDWILGHTNRFKAAVTHDGVFNLESMSLVTEELWFSGWEFGGMPWSEAAR
                     KQYAKWSPHLYAQNIRTPTLIITNELDFRVPVDQGLQMFTVLRGNGVPAEALVFPDEG
                     HWVLKPLNSKRWHDAVFGWMKKYLEK"
     gene            complement(5701..5776)
                     /locus_tag="PLB01_04395"
     tRNA            complement(5701..5776)
                     /locus_tag="PLB01_04395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5741..5743),aa:Lys,seq:ctt)
     gene            complement(5839..6801)
                     /locus_tag="PLB01_04400"
     CDS             complement(5839..6801)
                     /locus_tag="PLB01_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76572.1"
                     /translation="MITGFNTDVKHADRVYHVQTEDRGVANPVVESLVYVGGEILLSK
                     KSPYQDLITGDRVDEKALREMMDLQHRRVIEAIRRGRLDTGKIGEAPSDWADDTLPST
                     DRVSPAALAAVHAILAAPSEPLPGKPPVAASGKVSPRVSTGQTPAVPPPVPAASPAPP
                     APAPPPAPASPPPTVVAPRKPSSGASPASASGAAAKRPGSGMRSLDQVIVDYLASEAA
                     SEHLELSLTSDAELTSGGKVTLTLKATTSLTEKPLPGVHVSIRVVSTVRPPQILFRGV
                     TAGDGCVSSVCALPDIGTGNAALIIAASSALGNNEIKQLIKKKK"
     gene            complement(6831..9671)
                     /gene="uvrA"
                     /locus_tag="PLB01_04405"
     CDS             complement(6831..9671)
                     /gene="uvrA"
                     /locus_tag="PLB01_04405"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQQ76573.1"
                     /translation="MDSIVIRGAREHNLKNVSLSIPRNALVVLTGVSGSGKSSLAFDT
                     LFAEGQRRYVESLSAYARQFLEQMEKPDVDSIEGLSPAISIEQKTTSKNPRSTVGTVT
                     EIYDYLRLLYASVGKPHCPSCGREIAAQTIQQMVDRVLAMPEGTKFLLLAPFVRGKKG
                     EYRKQMGEMVKQGFLRARVNGEMVDLENAPELDKQKKHTIEVVVDRLVVRRDDDTRRR
                     LADSLETATKVGKGLVTIASLGATADAPTSDETLSTSYACPDCGTSLAEITPRLFSFN
                     SPFGACPTCSGLGSVQKVDPDRLVPDPSLSIRDGAIALYKPGSANWRLRQIEHLAKVL
                     KFSIDVPWSKLPKDVREIIFHGSGDREIKWKWKSEKGEYNWSSAFKGLVPILEAKYKE
                     VESEDARAEMENYMSASPCPDCKGRRLKPEALSVLVDGRSIDTLTALTLEDAQAFFTK
                     LKPTPREMEIAGKILKEIRDRLGFLNDVGIGYLSLSRASATLSGGEAQRIRLATQIGS
                     KLMGVLYVLDEPSIGLHAKDNRKLIDTLVAMRDLGNTVVVVEHDEETIRLADRVVDLG
                     PGAGIHGGEIVGEGTADELVKFPRSLTGKYLAGALRIDVPKIRRRGTGKTLAVVGAAE
                     NNLKDVTVRFPLGVFTAVTGVSGSGKSTLVNDILYRAAARLFHEATAKPGAHKKLEGL
                     EHLDKVIDIDQSPIGRTPRSNPATYTNVFNPIRELMAQVPEARMRGYGPGRFSFNVKG
                     GRCENCGGGGQIAIEMHFLPDIYVTCDVCGGKRYNRETLEVRYKGLNIADVLALTAEQ
                     AGEVFANVPPIKTIADTLNDVGLGYIQLGQPATTLSGGEAQRVKLARELARRATGRTL
                     YILDEPTTGLHFDDVKKLLNVLHALVERGNTVIVIEHNLDVVKTADWVVDLGPEGGAR
                     GGDLVAEGTPEQVAKSARSATGKYLAPLLARR"
     gene            9800..10828
                     /locus_tag="PLB01_04410"
     CDS             9800..10828
                     /locus_tag="PLB01_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76574.1"
                     /translation="MSTAAALVLAAGLAAGANPAPEGAQSTGEGSSVRAAAALPAPLL
                     ADPAADENRAPSARSALGATSSGRTAQSITPGTFQYARVLGTDFQPRTSATTYAYNFN
                     GCIYNVAGSDNRFVAHVDLPQGAILKYARFYFDDTSAPIDMTLWLTKYQPGVTSVDIT
                     SVSSSGSAGYGTTLSPEINPTNPPTEIVDNTSWSYALIVAPNALGAATSFCGARVAYF
                     PPPDGHFTAIAPCRVVDTRGGAPLGGGYLPASTERVYVMAGVCGIPANATGVSLNATA
                     TTVAGPGFLSLWQTGTAYPTVSTLNFNLGETQVNAAIVPLNASGQLSVAFGVNGGHLI
                     LDVNGYYY"
     gene            10853..12439
                     /locus_tag="PLB01_04415"
     CDS             10853..12439
                     /locus_tag="PLB01_04415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ76575.1"
                     /translation="MRDPAPADPPDETGRGWVVAVFAAAVLVRAAAAWPPGKFPADPD
                     ALLTGMRTLDILQARFHVFHTYVRFGCVESYLHAPFVALLGPTRLAVTFAPLIEGVLA
                     VMCLLALARRILPKPAALLATLFFAVPAARFFGATRLPTGYATVLACGVATLALAAIW
                     ADRPRAITAAALGLSAGLGLWCSILTLSCAAAAALWLLLAARRLPLRRLLLPAIAGFV
                     LGALPWIAFNAAHGFESLRNNYGATPTHSLASALSNLLYGLTGGFVELVGGPSSPGAI
                     PALAALAVAFHAIALVWFLAGGLRGEFGRAWALPALAALFSLVFFSISSAGNYRSATI
                     RFFVLCGPLVAVSAGALAWALWRRARIAGVLLTAGLVGLHLGGYEMPWSETRTLRREA
                     AADDARLLAFLEERRIGAIIGDYWSVYSFNYLSGGRVRAISADPAVDYHAYGDRLPAS
                     GVRWALVGDEPGVVERWAARAAPRGERFRVGRYTVLLPDPNPPGETPAEFQRRLQLAF
                     LLPERVVKADTKEWPLALWR"
     gene            complement(12462..13352)
                     /locus_tag="PLB01_04420"
     CDS             complement(12462..13352)
                     /locus_tag="PLB01_04420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ76576.1"
                     /translation="MNTRMPSISLVTPSLNQGKFIRATIESVLSQDYPNLDYRVQDGG
                     STDETLAVLREYEGRVPFVSEKDAGQADAINKGLSRATGEVLGYLNSDDVLRPGALAA
                     VGEAFASDPELMFVWGRAAYIDEAGKTMSPYLARPDAIERLADACFIAQPAAFFRRSV
                     WEDVGGFDASLHHTMDYDYWLRIAARYPASRTRFLDRELAGCRMHADAKTVAGWSRAL
                     IEIMDLVKRRTGYVSLWWCVAKWDHLTDGRSQATDPHPVPWRAYPPAIFEFLRRNPVG
                     LWGRGLRGGLKGLAKRLART"
     gene            complement(13349..14242)
                     /locus_tag="PLB01_04425"
     CDS             complement(13349..14242)
                     /locus_tag="PLB01_04425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ76577.1"
                     /translation="MKPPAVLVPAFRRAEASRRAIRSLLEAGAGRIVYVDDTGEAGGD
                     DIAREFPAVDVIRTTTPVWWTGAINLGIEAARGSSDAGVLFFNQDVTCAPDYFARLGE
                     AVDANPGALVGSAVMYAQEPGRVWSAGGAVEWWGRGIRVLHHGAPAAELPEAPFPADW
                     LFGMGTYVPVEVFDRVGLPDAGRFPMAWGDLDFSLRAKHAGIPVLVAPKARLFHEVGG
                     YDARVAGAPSVKQYAEWMADPKHNLSLSAHAEIWRRHGPRLLWPISLALRTIVLLLNF
                     ARIRLLFPSSPSRKSENGAPR"
     gene            complement(14239..15237)
                     /locus_tag="PLB01_04430"
     CDS             complement(14239..15237)
                     /locus_tag="PLB01_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQQ76578.1"
                     /translation="MARILVTGITGQDGSYLTESHLATGDDVHGIVRQSSLLQRTRLD
                     AIPALLAAREAGRLTLHYADLSDTSSLVAVVRNVNPDVVYHLAGQSHVKISFDLPEYT
                     ADSTGLGTLRLLEALRSVAPDARFFLAATSEIFGEPMESPQTEKTPMRPINPYAAAKV
                     YALNLVRIYRQAYGLFACSGILFNHESPRRGENYVTRKIARAAARIARGKQSELHLGT
                     LEPKRDWSYAPDIVDGMRRIVSAPKPDDFVLASGVTHSVGDFCRAAFAVVGLDHARYV
                     RLDPKYIRPIDISETRGDASKARRELGWAPKTGFDELVRLMVEAELAALDGRGEAA"
     gene            15266..16462
                     /locus_tag="PLB01_04435"
     CDS             15266..16462
                     /locus_tag="PLB01_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQQ76579.1"
                     /translation="MSTGRGLRVFVDLAPLSPDGGNGGARLFTLDLLSALLDRPRAHE
                     YHLLVKPHAQAVAAPLVERGAAFHRLGPGLDVEEPRKILRTRRRLHGFFQPLLDLAAP
                     DRSSLRRLGADVLFSPLGTAAFHETGLPHVVVAYDFQDLVYPQFFDADERRRRIEFRG
                     DLRRADRVVAISQATKLMAVERVHVPSERVSVLPPVAGPLRRPLDPRDALARLDALDL
                     FRTEYALYPANFWPHKNHERLLAAAARVARETPDFVLVLCGALDGARETLRARAEAAG
                     LGEVVRILPYLDEADTTALLEGAELLVFPSLFEGFGIPVLEAMALKTPVACSDLPALL
                     ELAGDAALFFDPTDEAGIAQSILRLWTEEETRDRLVARGLARAERYAKVDVAGAYHEI
                     LAAAAS"
     gene            16491..18830
                     /locus_tag="PLB01_04440"
     CDS             16491..18830
                     /locus_tag="PLB01_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76580.1"
                     /translation="MARRILLRSLALAAILAACFAHLVWNGGRAGKVVAENVPRPALS
                     SGTREIDLLHPTASFGHVYDLTFMWPEDRPVKVPEGSRLEVQLASNGRTRLLVVLPGH
                     PSASPSWRDVLRGRHRLQFVGDRGWIDLGPLPGQGTKLEVQVYGLGPGPFGFTEPHAA
                     GTGHFSGFQLRRPDSPRARPSLSIVRYRFRVADPVPAGETLARFFFFISCSRVLQVAG
                     FVALALLFVGWSWLWEGRFTRAVAALVPAVTLLHACGLAPLQGADEATHVGTVEAVLF
                     NPNLFENPAAFPKSIVQLYERLGYESWVGAREVPVTALLPENRPVTRAELSRPLTAEA
                     ATDGGARIDAVLISPRVRAPFYYNAFRLAGPLLRGQSVLDRVAAYVILSAAASLAFFL
                     AGLLIMSRTGLGALALAYGLVALWPYSVGVVASCSNYSLAIGIGQFLAACLVVGVLTP
                     SPRSRLLAAALFLAASLFGIGVWDDFVFVAVPAAAVLTILAAHAVIRMPAGSSRRTAM
                     GALTVAAALATGTILFALATGRVRRAISSFGARLPKEVGGFEDPSLWLLLAAAVAPLL
                     VSLVLALGAVRGRIVSEGGRRRAARVRSAALAALFVAMFLATRWTSVPFEAARLDYPD
                     EVAAHWSAFWSNNFAFDQDVLSWKMYWGVFGYADVSYPDALYALARWACVAFFLALPL
                     LSWRFTLRSPNRSALFLVACGYALTVCVVTNSLRYFQPTNPWGRFILPVLSLVALPLL
                     VRTDPPEHDRSWRLAVAVFVVLHVWTATVLLGSRYALGL"
     gene            complement(18827..20812)
                     /locus_tag="PLB01_04445"
     CDS             complement(18827..20812)
                     /locus_tag="PLB01_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQQ76581.1"
                     /translation="MATRPDDSTGPVTGSRRLRTPSGVGVGILARLKNRPVQYLLDVL
                     VLSGAFLVAYAIRFDFRVSAEMFRHAMTQLPLVVLLQFAALSAFGAYAFIWRYVGLAE
                     TLVFARAAVAATVPLLVMRLALPETLQDYRVPFSIIVMDGLLGFGGVLGLRVGRRFTW
                     ERWERGRRPAGAAEQKRVILVGAGRAGVLALREILGRGDAELNVRGFVDDDPAKQDSL
                     IQGVRVLGTTAELPGLVRHLAIDEVVITIVGAPRRDIRRIVKTCEEIPVRVRIMPGIW
                     EILQGNVEVSGIRDVQIEDLLGREPVQLDEEELRRFLVGAAVMVTGAGGSIGSELARQ
                     VARLGPRQLVLVERAEFALFDVDREIRRRWPDSRPAAVVADIGDEARMRRVFETYRPR
                     IVLHAAAHKHVPLMEENASEAIRNNVLGTARLGTFAGEFGVDVFVMISTDKAVRPTSV
                     MGASKRVAEIVVQDLDRRYGTRFVAVRFGNVLGSAGSVIPIFREQIRRGGPVTVTHPD
                     MVRFFMTIPEASQLVLQAAAMGKGGEIFVLDMGEPVRIVDLAHEMITLSGLKPNEDID
                     VVFTGIRPGEKLFEEVGTDAENVSPTGRPKILIGKISPSPPEDVERALEELRRLADEG
                     GEDAIRRCLGEILPDAQLVGAARPLAAGQKRPGGNAG"
     gene            20878..22623
                     /locus_tag="PLB01_04450"
     CDS             20878..22623
                     /locus_tag="PLB01_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQQ76582.1"
                     /translation="MHSGWARMLLALSLAAPWPLLLGRGPNPEPPPARPVFEEVAARS
                     GLDFVHFNGATGQDTLAEIAGSGAALFDYDGDGDLDVYLVQGTILDGPLAKAVVPWRG
                     KGRPVGRLFRNDLVVRPDGSRHLRFTDVTEKAGLAFEGYCMGAATGDFDNDGRPDLYV
                     TCLGSNHLFRSRGDGTFEDVTKKAGADDPRWSTSAAFVDYDRDGFLDLYVANYVDFET
                     DPKKVCLSPSSAREYCGPQAYAPVPHRLLHNRGNGTFEDVSERSGIGKERGSGLGVVA
                     ADLDGDGWPDLMVANDGNPNFLFFNQHDGTFRNDALMAGVAVNAMGRAMAGMGVDAGD
                     FLGDGREHVFITNIMQDPAALFVNEGDGLFSDRTIASGLVPGTLGKTGFGTGWFDWDN
                     DGWLDLVVTNGAVLKIPEQHRAGDPLPLRQPNVLFRNTGSGTFVDATIMAGRAFATPN
                     VGRGIAFGDIDNDGTTDLLITSNGGPARLFLNRAGLRNRWVGLRLLARSGRDALGARV
                     EVRRPGLPSLWRRAHTDGSYLCASDPRVLVGLGPTGTPRGLHVIWPDGTSETWPAPPT
                     GRYVTIRQGRSPAFE"
     gene            22627..24210
                     /locus_tag="PLB01_04455"
     CDS             22627..24210
                     /locus_tag="PLB01_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ76583.1"
                     /translation="MPRGISSFLLLASLFFAAAGARGERRPFLETVPFPPGSALEPAV
                     AAQIHGVEKNLAALRTADGVSPDALAEAYADLGQLFHAYELWDSAAASYRNAIRLAPR
                     DIRSIHLLAEVAMKTGDLEEARRRHAQVLALQPADLASRVGLGEALLGLNRPQDARRE
                     FLDATRISPGSAAAHAGLGRTALVQRDFSEARTRIEIALRLAPEATRLHYELAMAWRG
                     LGNRDEAARELARAGTVGVRVADPLLEELSALRRGERTHLVRGRLAFVNGRMREAADE
                     FAAAVEAEPRGIAGLVGLGSALAMLGDTDRALDAFDRALAIAPANVAASYNVGRVLLA
                     RGDFAGASRHLRRVVEAEPEGADGVRLLARSLAAQGDTAEALRLYETAVRLAPQDEDS
                     ALGHADVLARLGRFADARDALEDGHRRMPDRGRLALALAYLLAASPETSVRDGRRALE
                     LAQVVYAASPAPDSGRTVALALAELGRCAESEKWLDRLLGTFGMDGAADMSALRADRD
                     RAARGAPCRPPGRPPRAAP"
BASE COUNT         3388 a         8694 c         8188 g         3944 t
ORIGIN      
        1 gcgctcgcgg cggccggcgc ccacgccgag gacgcccctc ccgccgcacg gttccggctg
       61 tttccggacg ccgcggcacc cgccgagccg aagccggcgg gcggagccgc ctccggcggg
      121 agggagaagg cgtcgacgag gcgattctgg ctcaccgcgg ccgagatcgg gatcctcgag
      181 ttcctgccct acgcgcagaa ccgcttcgtc aacgacgagg actacgcccg gatctccgtc
      241 gagtccatcc ggaagaactt cgacgcgggc ttctcgttcg acaacgacaa attcacgacg
      301 aaccagctcg ggcacccgat cgcgggcggg ctgttcttca acgccgcccg gacgaacggc
      361 tactcgttct gggagtcggc tccgttcgtc ctcgccggca gcactttctg ggagatgttc
      421 ggggagacac agggcccctc gctcaacgac ctcgtgaaca cgacgctcgg cgggatcacg
      481 ctcggcgagg cctcgtaccg gctctcgcag atgatcctcg acgaccgcgc ccgcggcggc
      541 gcgcgcatcg cccgcgaggc cctcgcgggc ctcgtgaacc ccacgcagct cctcacgcgc
      601 gtcttcaccg gcgaagcctg ggccgtccgc tccgggcgcg gggaatacgt cgaaccttcg
      661 cgtttcgtca cggagctgga cgcgggcggg cgccacttcg tctcgagcac gcgcacgaac
      721 cccgaccagg ccgtcctggc cgccgccatc cggtacggcg acccgttcga ccgggccgtc
      781 tcgcgcccct tcgactcttt cgacctcgga gtcgacatgt cgtatccaag ctcggcatgg
      841 ctcacgcgcg tcgagatccg cggcctcctc ggcggctggg acgtcgaccc cggcgagacc
      901 ggcggccggc acgtcttcgg cgtcttcatg gacttcgact acacgaacaa cgacacccga
      961 atgttcagct cgcagtcgtt ccggttcggc ctcctgtcgc tgcgccccct cggtggcggc
     1021 gtcgagctgc gcggcgaggc gctcggcgcc gtcgtgcccc tcgcggcgct gaagaacgac
     1081 tacctcgagg agtcgagcgg gctcgtcggc cgcgcgtacg actacggccc gggcgccggg
     1141 atctacaccg cgatccggtt ccgtcgccgc gagctcgacc tcgcgacgct gacgtattcg
     1201 gtcttctgga tgcacacgtc gaacggaatc gggcggaacg cgagcctcca gtccttccgg
     1261 gtcgaagggc ggatacccgt cgccgggccg ttcgccgtcg gcggctcgtg gacgtgggga
     1321 aagaggatca cgacgtacga cgactacgcc acgtaccgga gcgacgccac gcagtggcgc
     1381 gcgttcgcgt cggtcatgtt ccgctagagc cggggcgcta cgccgggacc ttgagctcct
     1441 cgcggcggcg gatcacgagg tcgatcaggc cgtactcctt cgccgcgtcc ggctcgaaga
     1501 tgttgtcgcg gtccgtgtcc cgggagatct tctcgacggg ctggcccgtg tggtgggcga
     1561 ggatctcgtt gaggcgctgc ttggtgcgca gcatctcgcg cgcctggatc tcgatgtcgg
     1621 aggcctgtcc ctcggcgccg ccgtgcggct ggtggatgag gacccgcgca ttcggcagcg
     1681 ccgcgcgctt gcccttggcg ccgcccgcga ggaggagcgc gcccatcgag gccgcctgcc
     1741 cgaggcagta ggtcgcgacg tcgcacttca cgtactgcat ggtgtcgtag atcgcgaggc
     1801 ccgccgacac ggacccgccg ggcgagttga tgtagagcgt gatgtccttc tcggggtttt
     1861 ccgcctcgag gaagagcatc tgcgcgatca cgagattcgc gacgtcgtcg ttgatcgccg
     1921 agcccaggaa gatgacgttg tccttgagca ggcgcgaata gatgtcgtac gcgcgctcgc
     1981 cgcggttcgt ctgctcgatg accatcggaa tcagcatggc gtggcctcca acgacccgta
     2041 gtgtactcag cccgcgacga ccgcttcggc gacgaggaag tcgaccaccc ggtcgatccg
     2101 gagctcctcg cgcacgccct cgacgcggtc cgccttcgcg agcgcgccct tgagctccgc
     2161 gaacgacatc cccatggcct gcgcgcggcg cttgagctcg gcgtcgagct cggtgtcggt
     2221 cacggagaga ctctccttct cgccgatcgc gtcgaggagg aggtactcct tgacgcggcg
     2281 ctcggccgag ggacgggctt ccttgcggag ctcgttccag tcgatcttcg cgtccttgag
     2341 gtcgacgccc tgccgcgcca tgtggcgcgc gtactccttg agggccgagt cgatctcgga
     2401 ctccaccatg gactcgggcg cgggcaccgc gttcagcgcg agaaggccgt ccagcgcggc
     2461 gcggcgccgc tgctcgcgca cggtctgctc tttctccttc gccagggatt cctggatctt
     2521 ctcgcggagc atcgcgagcg acgcgccctc gggcgcctcg ccctcgcgcg gggtgagcgc
     2581 gaggcgcgcg agctcgtcgt cgagggccgg aaggacgcgc gtcttgatcg cgacgagatg
     2641 caccgtgtag aggaccgtct tccccgcgaa ttccgagtcg gagacgtccg ccgcgaagct
     2701 cttctggaag ctgaccgtgg cgccggcctc ggcgttgcgc aggtgggccg acagctcggg
     2761 aagcgtgccc tcgccgccga tctcgacgag gaccttctcg cggttgaagt cctcgccgtc
     2821 accggcgggg aacgtgccct tgatgtcgac gagaacgtgg tcgccgtcct tcacggggcg
     2881 gccctcgacg gcgtcgtacg tggcatgccc ctcgcggatg cgcgaaagca cgcggtcgac
     2941 gtcctcggcc gtgacggccg tcgaaccctc cggcaccttc aggccgcggt agtccttggg
     3001 ctccaccgcc gggcgcacct cgaggtcgac cttgaacgcg agcggggcgt cgaactcgaa
     3061 cttgaggtcc gcgatcttcg gcggaccgag cgggatgagg ttcttctcgc ggagggctcc
     3121 cgcgagggat tcctggatga ggcgctcgag cacctcgccc ttgatctcct cggcgaaccg
     3181 ctggcgaatg atgtgcgcgg gggcctttcc ggggcggaag ccggggaggc ggacctgctt
     3241 cgcgaagacc ttgatcgtct tctcggtcgc cgccttcacg tcctcggcgg ggacctcgaa
     3301 ggtcaggact ttcttcgtgg gggactcgtc gacgaaagag gtgagcatcg tgtgtccttg
     3361 tcgttcgttt cggggaagct ggtgcgggag gagggactcg aaccctcacg ggtcgcccca
     3421 cgggatccta agtcccgcgc gtctgccaat tccgccaccc ccgcgggccc ggaggggcgg
     3481 acgttagctc atttctcgag gtatttcttc atccagccga acacggcgtc gtgccagcgc
     3541 ttgctgttga ggggcttgag gacccagtgc ccttcgtccg ggaacacgag cgcctcggcc
     3601 ggaacgccgt tgccgcgcag gaccgtgaac atctgcaggc cctggtcgac ggggacgcgg
     3661 aagtcgagct cgttcgtgat gatgagcgtc ggcgtcctga tgttctgggc gtacagatgc
     3721 ggcgaccact tcgcgtactg tttccgggcc gcttcgctcc acggcatgcc gccgaattcc
     3781 cagcccgaga accagagctc ctcggtcacg agggacatcg actcgaggtt gaagacgccg
     3841 tcgtgcgtga cggccgcctt gaagcggttc gtgtggccga ggatccagtc cacggcgtac
     3901 ccgccgtagc tcgccccggc gatgccctgc cgggacgcgt cgacgaacgg cagcttcacg
     3961 accgcgtcga agacggcgtc gaggtcgacc atgaccttgc cgccccagtc gcccgagatc
     4021 tcgtccacga acttctggcc gaagcccgtc gagccgcgcg ggttcggggc cacgacgacc
     4081 cagccctgcg ccgcccagag cgtggggttc cagcgcgcgg accagccgtc ctcccacgcg
     4141 ccctgcgggc cgccgtggat caggaagacg accgggtact tcttcgccgg gtcgaacatc
     4201 ggcggcttca cgagccagta ctgcacgcgc gcgccgcccg cgcccgtcgt cgacaggctc
     4261 tcgggcgccg acatcgcgac gtccttccgc caggagcggt tctcgtcggt cagcgccttc
     4321 ggagaagagg acgaagacga agaagaggat ctttcttcga gtggtaaaga gaagatctcc
     4381 gacggcgccg tcagcgtgtt cttcgtgaac acgatgcggg agggtccgac ccggagcgcg
     4441 ccgatgttgc cgcccctcag gatttcgcgg ggcgtgcccc ccgagaggga cacttcgaag
     4501 aaatcccttc ttccctctct ctcggccgtg aaatacacgg tcttgccatc cttcgacagt
     4561 gcgaattcgt ccacggagag gtccgggttc tcgaagaggg ttctcttcgc cttcgtcttc
     4621 cggtcgtaga cgtcgagata ccagcggtcg ccctcgaacg tcggccggcg ctgagcgcgg
     4681 acgacgatcg aagcgccgtc gggcgtgtac gtcgggtcga agtcggcagc cgggttgccg
     4741 gccgtgagct tcacggcctc gccgcccgcc gcggggaccg tccagacgtc cttgttcgtc
     4801 gtgaacgctt ccttgtccgc gccgtcgcgg ttcgaaacga acgcgatctc cttgccgtcc
     4861 ggagaaaagg cgatcccgcc gtcctcgtag tggttcggcg gcgagtcgaa gtcgcccggc
     4921 gtgacgtccg tgagagcgcc cgacgcgacg tcggcgacga agacgtggtg ccggacgttc
     4981 tcccgccatt cgtcccagtg gcggtggagg aggcgcgtga ggcggcggac cttcacggga
     5041 tccttttcga ggtcctcgcg ctttttcttg ctgcaggcct cgtccgcaca ctcgggatac
     5101 acgtccgaca cgaacgcgag cttcttgccg tccggcgaga agacgagagg gtccgccaca
     5161 ccggccgaga ggtccgtcag cttgcgcggc tcgccgcccg agaggtcgag gagccacacc
     5221 tgcggcgacc cggagcgcgt cgagacgaag gcgagcgtcc tgccgtccgg cgagaagcgc
     5281 ggggcgttgt cggccttctc gtggcgcgtc agggcgcgcg gcggggccga gccgtcggcg
     5341 ggcacgagcc agacgtccgc gttgcgcttg ccggcggcga ggtccgtcgt cgtccggacg
     5401 aacgccaccc acttgccgtc gggcgacagc tgcgggtcgg cgacgcgcac ggtcaggagg
     5461 ttgtcctcga tcgtcatcgg ccgggcggcg aacgcgggaa tggagagaac ggaaagagag
     5521 agaagaagga ggtttcttag aaggtgaaga gggcggcggg gcgcgacggg gcgcgacggg
     5581 cgggcgcagc ggctcggaat ctctctctta ctcattctag gaatcccctc ttctcttcat
     5641 tcttttcgtc ttcagccaag aaaaaggccg gccgttttca cggccggcct ggtcacgaga
     5701 tggtgagccg agagggactt gaacccacaa cccagagatt aagagtctct tgctctgcca
     5761 attgagctat cggcccatgc tccgcataac ggagcggccg cggaatatac cccggcccgc
     5821 ccgcggcttc aagcggcgtc acttcttctt cttgatgagc tgtttgatct cgttgtttcc
     5881 gagggcggac gaggccgcga tgatcagggc ggcgttgccc gtcccgatgt ccgggagggc
     5941 gcacaccgac gagacgcagc cgtccccggc cgtcacgccc cggaacagga tctgcggcgg
     6001 cctcaccgtc gagacgacgc ggatcgacac atggacgccg gggaggggct tctccgtgag
     6061 cgacgtcgtc gccttgaggg tcagggtcac cttgccgccc gacgtcagct cggcgtccga
     6121 cgtcagcgag agctcgaggt gctcggatgc ggcttccgac gccaggtagt cgacgatcac
     6181 ctggtccagc gagcgcatcc cggatcccgg gcgtttcgcc gcggcgcccg aggcggacgc
     6241 gggcgacgcc ccggacgatg gcttgcgcgg ggccaccacg gtgggcggcg gggacgccgg
     6301 cgccggcgga ggcgccggag cgggcggcgc cggcgaggcc gcaggaacgg gaggagggac
     6361 cgcgggggtc tggcccgtcg aaaccctcgg agagaccttt ccggaagccg caacgggcgg
     6421 cttgcccggc aggggctcgg acggcgccgc gaggatggcg tggacggccg cgagcgccgc
     6481 cggagagacg cgatcggtag acggcagcgt gtcgtcggcc cagtcggacg gcgcctcgcc
     6541 gatcttcccc gtgtcgaggc gcccccggcg gatcgcctcg atgacccgcc ggtgctgcag
     6601 gtccatcatc tcccgcagcg ccttctcgtc cacgcggtcg ccagtgatga ggtcctggta
     6661 ggggctcttc ttggacagca ggatctcgcc gcccacgtac acgagcgact cgacgacggg
     6721 gttcgcgacg ccgcgatcct ccgtctgcac gtggtacacg cggtccgcgt gcttcacgtc
     6781 cgtattgaac ccggtgatca cgtgaccgca aattctagcg gaaacgccgt ttagcgccgc
     6841 gcgaggagcg gggccaggta ctttccggtg gccgagcggg cgcttttcgc gacctgctcg
     6901 ggcgtcccct cggcgacgag gtcgccgccg cgggcgccgc cctcgggccc gaggtccacg
     6961 acccagtccg ccgtcttgac gacgtcgagg ttgtgctcga tcacgatgac ggtgtttccg
     7021 cgctcgacga gcgcgtggag aacgttcagg agcttcttca cgtcgtcgaa gtggagaccc
     7081 gtcgtcggct cgtcgaggat gtagagggtg cggcccgtgg cccggcgcgc gagctcgcgc
     7141 gcgagcttca cgcgctgcgc ctcgccgccc gagagcgtcg tcgcgggctg tccgagctgg
     7201 atgtacccga ggccgacgtc gttcagcgtg tcggcgatcg tcttgatggg cgggacgttc
     7261 gcgaagacct ctccggcctg ctcggccgtg agcgcgagga cgtccgcgat gttcagcccc
     7321 ttgtagcgga cctcgagcgt ctcgcggttg tagcgcttgc cgccgcagac gtcgcacgtc
     7381 acgtagatgt cgggcaggaa gtgcatttcg atggcgatct gcccgccgcc gccgcagttc
     7441 tcgcaccggc cgcccttcac gttgaacgag aaccgcccgg gcccgtagcc gcgcatccgc
     7501 gcctcgggaa cctgagccat gagctcgcgg atcgggttga agacgttcgt gtacgtcgcg
     7561 gggttcgagc gcggcgtgcg cccgatgggc gactggtcga tgtcgatgac cttgtcgagg
     7621 tgctcgaggc cctcgagctt cttgtgcgcg cccggcttcg ccgtcgcctc gtggaagagg
     7681 cgcgcggccg cgcggtagag gatgtcgttc acgagcgtcg actttcccga gcccgagacg
     7741 cccgtgaccg ccgtgaagac gccgagcggg aacctcacgg tcacgtcctt gaggttgttc
     7801 tccgcggccc cgacgacggc aagagtcttt cccgtccccc gccggcggat cttcgggacg
     7861 tcgatgcgga gggcgccggc aaggtacttg cccgtgagcg agcgcggaaa cttcaccagc
     7921 tcgtccgccg tgccttcccc cacgatctcc ccgccgtgga tgccggcgcc gggcccgagg
     7981 tccacgacgc ggtccgcgag gcggatcgtc tcctcgtcgt gctcgacgac gacgaccgtg
     8041 ttcccgaggt cgcgcatcgc gacgagcgtg tcgatgagct tgcggttgtc cttcgcgtgg
     8101 aggccgatcg agggctcgtc gaggacgtag aggacgccca tgagcttcga cccgatctgg
     8161 gtcgcgagcc ggatgcgctg cgcctcgccg cccgagagcg tcgcagaggc ccgcgagagc
     8221 gacaggtacc cgatcccgac gtcgttcagg aaaccgaggc ggtcgcggat ctccttgagg
     8281 atcttcccgg cgatctccat ctcgcgcggc gtgggcttca gcttcgtgaa gaacgcctga
     8341 gcgtcctcca gcgtgagcgc ggtgagcgtg tcgatcgagc ggccgtccac gaggacggag
     8401 agcgcctcgg gcttcagccg ccgccccttg cagtccgggc agggcgaggc cgacatgtag
     8461 ttctccatct cggctcgggc gtcctcgctc tcgacttcct tgtacttcgc ctcgaggatc
     8521 gggacgagcc ccttgaaggc cgaagaccag ttgtactcgc ccttctccga cttccacttc
     8581 cacttgatct cgcggtcgcc cgatccgtgg aagatgattt cgcgcacgtc cttcggcagc
     8641 ttcgaccacg gcacgtcgat cgagaacttc aggaccttgg cgagatgctc gatctgcctc
     8701 aggcgccagt tcgcggagcc cggcttgtag agcgcgatcg cgccgtcgcg gatcgagagc
     8761 gacgggtccg gcacgagccg gtccgggtcc accttctgca ccgacccgag gccgctgcac
     8821 gtcgggcagg cgccgaacgg ggagttgaag gaaaagaggc gcggcgtgat ctccgcgagc
     8881 gacgtcccgc agtccgggca ggcgtagctc gtcgagagcg tctcgtcgga ggtcggcgcg
     8941 tcggcggtcg cgccgagcga cgcgatcgta acgaggccct tcccgacctt cgtcgccgtc
     9001 tcgagcgagt ctgcgagccg tcggcgcgtg tcgtcgtcgc gccggactac gaggcggtcc
     9061 acgacgacct cgatcgtgtg cttcttctgc ttgtcgagct cgggcgcgtt ctcgaggtcg
     9121 accatctccc cgttcacgcg ggcgcgcagg aagccctgct tgaccatctc gcccatctgc
     9181 ttccggtact cgcccttctt cccgcggacg aacggggcga ggaggaggaa cttcgtcccc
     9241 tcgggcatcg cgaggacgcg gtcgaccatc tgctggatcg tctgggccgc gatctcgcgc
     9301 ccgcacgacg ggcagtgggg cttgccgacg gaggcgtaga ggaggcggag gtagtcgtag
     9361 atctccgtca cggtcccgac cgtcgagcgc gggtttttcg acgtcgtctt ctgctcgatc
     9421 gagatcgcgg gcgagagccc ctcgatcgaa tcgacgtcgg gcttctccat ttgctcgagg
     9481 aactgccggg cgtaggccga gagggactcg acgtaccgcc gctgcccctc ggcgaagagc
     9541 gtgtcgaacg cgagcgacga cttcccggac cccgacacgc ccgtcaggac gacgagggcg
     9601 ttgcggggga tcgagaggga gacgttcttg aggttgtgct cgcgggcgcc gcggatgacg
     9661 atggagtcca tgggggccgg gattatggac cgggtctaaa cggcgcgcct tgcctcgcct
     9721 ctgtctcgga cctatgttcg acgaaagtcc gccggtccgg gctcaaggag gtcgacttat
     9781 gtcgtctcgt gtcgcctatt tgtccactgc cgccgcgctc gtccttgccg ccggcctcgc
     9841 ggctggcgca aaccccgctc cggaaggggc ccagagcacg ggtgagggca gcagcgtccg
     9901 ggccgccgcc gctcttccgg ccccgctact cgccgaccct gccgccgacg agaacagggc
     9961 cccgtccgct cgaagcgccc tcggagcgac gtcgagcggg cgaactgccc agtccatcac
    10021 gcccggcacc ttccagtacg cgcgtgtgct cggcaccgac ttccagccgc gcacgtcggc
    10081 gacgacctac gcctacaact tcaacggctg catctacaac gtcgccgggt ccgacaaccg
    10141 gttcgtcgct cacgtcgatc tcccgcaagg ggcgatcctc aagtacgcgc gcttctactt
    10201 cgacgacacg tccgcgccga tcgacatgac gctctggctc acgaagtacc agccgggcgt
    10261 cacgagcgtg gacatcacga gcgtcagctc ctccggttcg gccggctacg ggaccaccct
    10321 gagccccgag atcaacccca ccaacccgcc gaccgagatc gtcgacaaca ccagctggtc
    10381 gtacgccttg atcgtcgcgc cgaacgccct cggggccgcg acttcgttct gcggagcccg
    10441 cgtcgcctac ttccctcctc cggatgggca cttcacggcg atcgcgccct gccgagtcgt
    10501 cgacacgcgc ggcggcgcgc ctctcggcgg cggctacctg ccggcctcga ccgagcgcgt
    10561 ctacgtgatg gcgggcgtct gcggcattcc cgcgaacgcc accggcgtct ccctgaacgc
    10621 cacggccacg accgtcgccg gcccgggctt cctgagcctc tggcagacgg gaacggctta
    10681 cccgaccgtc tcgacgctca acttcaatct cggtgagacg caggtgaacg ccgccatcgt
    10741 cccgctgaac gccagcggac agctctccgt cgcgttcggc gtcaacggcg ggcacctcat
    10801 cctcgacgtg aacgggtact actactgaga acgacctaaa ctcccgcgcg acatgcgcga
    10861 ccccgccccg gccgatcctc ccgacgagac gggccgcggc tgggtcgtcg ccgtcttcgc
    10921 ggccgccgtc ctcgtccggg ccgcagccgc ctggccgccc ggaaagttcc cggccgaccc
    10981 ggacgcactg ctgacgggga tgcgcacgct cgacatcctc caggctcgat tccacgtctt
    11041 ccacacgtac gtgcgcttcg gctgcgtcga gagctacctg cacgctccgt tcgtcgccct
    11101 gctcgggccg acgaggctcg ccgtcacgtt cgcgccgctg atcgagggcg tcctcgccgt
    11161 gatgtgtctg ttggcgctgg cgcggcgcat tctgccgaaa ccggcggcgc tcctggcgac
    11221 cctcttcttc gccgtgcccg cggcccgctt cttcggcgcg acgcgcctgc ccacgggcta
    11281 cgccaccgtt ctcgcgtgcg gcgtcgcgac gctcgctctc gcggccatct gggccgaccg
    11341 accgcgcgcg atcaccgcgg cggcgctcgg gctctccgcc ggcctcggcc tctggtgctc
    11401 catcctgacg ctgagctgcg ccgcggcggc cgcgctctgg ctgctgctcg cggcccgccg
    11461 cctcccgctc cggcgcctcc tgcttccggc gatcgcaggg ttcgttctcg gcgcgctgcc
    11521 gtggatcgcc ttcaacgcgg cgcacggatt cgaatctctc cgcaacaact acggcgcaac
    11581 gccgacgcac agcctcgcgt ccgcgctctc gaatctcctc tacggcctca cgggaggctt
    11641 cgtcgagctc gtcggcggtc cgtcctcgcc gggcgcgatt ccggcgctgg cggccctcgc
    11701 cgtcgcgttc cacgcgatcg cgctcgtgtg gttcctggcg ggaggcctgc gcggagaatt
    11761 cgggcgcgcg tgggcgctcc ccgctctcgc cgcgctcttc tcgctcgtct tcttcagcat
    11821 ctcatccgcc ggcaactacc gctccgcgac catccggttc ttcgtcctct gcgggcccct
    11881 cgtcgcggtc tcggcgggcg cgctcgcgtg ggcgctctgg cgccgggcgc ggatcgccgg
    11941 cgtcctcctc acggccggtc ttgtcggtct ccatctgggc ggctacgaga tgccgtggtc
    12001 ggaaacgcgc acgctccggc gcgaggccgc cgcggacgac gcgcgcctcc tcgccttcct
    12061 cgaggagcgg cgcatcggcg cgatcatcgg cgactactgg tcggtctact ccttcaatta
    12121 cctgtcggga gggcgcgtgc gggcgatctc ggccgacccg gccgtggatt accacgctta
    12181 cggcgaccgc ctcccagcga gcggtgtccg ctgggcgctc gtgggagacg agcctggagt
    12241 cgtcgagcgc tgggccgcgc gcgccgcgcc ccggggcgaa cggttccgcg tcggccgcta
    12301 cacggtcctc ctgcccgacc cgaacccgcc cggggagacg cccgccgaat tccagcgccg
    12361 actccagctc gcgttcctgc tgccggagcg agtcgtcaag gcagacacca aggagtggcc
    12421 gctcgcgcta tggcggtgag agacgcggcc cccgggaagg atcaggttcg cgcgagccgc
    12481 ttcgcgagtc ctttgagccc acccctcaag ccgcgccccc agagccccac cggattccgc
    12541 ctcaggaact cgaagatcgc cggcggatac gcccgccacg gcacgggatg cgggtcggtc
    12601 gcctggctgc ggccgtccgt gaggtggtcc cacttcgcga cgcaccacca gagcgacacg
    12661 tagcccgtgc ggcgcttcac gagatccatg atctcgatca acgcccggct ccaaccggcc
    12721 acggtcttcg cgtcggcgtg catccggcag cccgcgagct cgcggtcgag gaagcgtgtg
    12781 cgcgaggccg ggtaacgggc cgcgatccgg agccagtagt cgtagtccat cgtgtggtgg
    12841 agcgacgcgt cgaatccgcc gacgtcctcc cacacgctgc gccggaagaa cgccgccggc
    12901 tgcgcgatga agcacgcgtc ggcgagacgc tcgattgcgt cgggtcgcgc gagatacggc
    12961 gacatcgtct tgccggcctc gtcgatgtag gccgcgcggc cccacacgaa catcaactcc
    13021 ggatccgacg cgaacgcttc tcccaccgcc gcgagcgcgc ccggccgcag gacgtcgtcg
    13081 ctgttgaggt atcccagcac ctcgcccgtc gcgcgcgaga ggcccttgtt gatggcgtcg
    13141 gcctgacccg cgtccttctc cgatacgaat gggacgcgtc cctcgtactc gcgcagcacg
    13201 gcgagcgtct cgtccgtcga gcccccgtcc tgcacgcggt agtccaggtt cgggtagtcc
    13261 tgcgagagaa cgctctcgat cgtcgcccgg atgaacttcc cctggttcag agaaggggtc
    13321 acgagcgaga tcgacggcat gcgggtgttc atcggggggc gccgttttcg gatttccgag
    13381 aaggtgaaga ggggaaaagc aggcggatgc gtgcgaagtt cagcaggagc acgatcgttc
    13441 tcagcgccag ggagatcggc cagagcagcc tcggtccgtg gcgtctccag atctccgcgt
    13501 gcgccgagag cgagaggttg tgcttcgggt ccgccatcca ttccgcgtac tgcttcacgc
    13561 tcggcgcacc cgccactcga gcgtcgtagc cgccgacctc gtgaaagagc cgggccttcg
    13621 gtgccacgag caccggaatg cccgcgtgct tcgcccgcag cgagaagtcg aggtcgcccc
    13681 aggccatcgg gaagcgcccc gcgtcgggca ggccgacgcg gtcgaagacc tcgacgggca
    13741 cgtacgtccc cattccgaac agccagtcgg ccgggaacgg cgcttccggg agctccgccg
    13801 ccggcgcccc gtggtgcagc acgcggatgc cacggcccca ccactccacg gcgccgcccg
    13861 ccgaccacac gcgcccgggc tcctgcgcgt acatcaccgc gctgcccacg agggcgcccg
    13921 ggttcgcgtc gaccgcttcc ccgagcctcg cgaagtagtc cggcgcgcac gtgacgtcct
    13981 ggttgaagaa gaggacgccc gcgtcggagc tcccgcgcgc ggcttcgatc ccgaggttga
    14041 tggcgcccgt ccaccagacc ggcgtcgtcg tgcggatcac gtccaccgcc gggaactcgc
    14101 gcgcgatgtc gtctccgccg gcctcgccgg tgtcgtcgac gtagacgatg cggcccgcgc
    14161 cggcctcgag gagcgagcgg atcgcccggc gcgacgcctc cgcgcgccgg aaggcgggga
    14221 cgaggacggc cggaggcttc aggcggcttc cccgcgcccg tcgagcgcgg cgagctcggc
    14281 ctccaccatg aggcgcacga gctcgtcgaa gcccgtcttc ggggcccagc caagctcgcg
    14341 gcgcgccttc gaggcgtcgc cccgcgtctc actgatgtcg atcgggcgga tgtacttggg
    14401 gtccagccgg acgtaccgcg cgtggtcgag cccgacaacg gcgaacgcgg ccctgcagaa
    14461 gtcgcccacc gaatgtgtga cgcccgaggc gaggacgaaa tcgtccggct tcggggccga
    14521 gacgatgcgg cgcatcccat ccacgatgtc cggcgcgtag ctccagtccc gctttggctc
    14581 gagcgtcccg aggtgcagct ccgattgctt tccgcgcgcg atccgcgccg ccgcgcgcgc
    14641 gatctttcgg gtgacgtagt tttcgccgcg gcgcgggctc tcgtggttga aaaggatgcc
    14701 cgagcacgcg aagaggccgt aggcctgccg gtagatccgg acgaggttca gggcgtagac
    14761 cttcgcggcc gcgtatggat tgatgggccg catgggcgtc ttctccgtct ggggcgactc
    14821 catcggttcg ccgaagatct cgctcgtggc cgcgaggaag aaccgggcgt ccggagcgac
    14881 gctgcggagc gcctcgagca gcctgagggt gccgagaccg gtcgagtcgg ccgtgtactc
    14941 gggaagatcg aacgaaatct tcacgtggct ctgcccggcg aggtggtaga cgacgtcggg
    15001 attcacgttt cgcaccacgg ccacgaggct gctcgtgtcg gaaaggtcgg catagtggag
    15061 cgtcaggcgc ccggcctccc gggccgccag cagggccggg atcgcgtcga ggcgcgtccg
    15121 ctgcaggagg ctcgattgcc ggacaatgcc gtgcacgtcg tccccggtcg cgaggtgcga
    15181 ctccgtgaga tacgacccgt cctggcccgt gatgcccgtg acgaggatgc gcgccatggc
    15241 cccgagtttg tgcgaaagta gggcgatgtc aacgggacgg ggcctccggg tcttcgtcga
    15301 cctcgctccc ctttcgcccg acggcggaaa cggaggggcg cgcctcttca cgctcgacct
    15361 cctctccgcg ctgctcgacc ggccccgggc gcacgaatac caccttctcg tgaagccgca
    15421 cgcgcaggcc gtcgcggcgc ctctcgtcga gaggggcgcc gccttccacc gcctcgggcc
    15481 cggcctcgac gtcgaggagc cgcgcaagat cctccgcacg cgccggcgcc ttcacggctt
    15541 tttccagccc ttgctcgacc tggccgcgcc cgaccgctcg tccctcaggc ggctcggcgc
    15601 cgacgtgctc ttcagccctc tcggcaccgc ggccttccac gagacggggc ttccccacgt
    15661 cgtcgtcgcg tacgacttcc aggacctcgt ctatccgcag ttcttcgacg ccgacgagag
    15721 gcgacgccgc atcgagttcc ggggcgatct gcgccgggcc gaccgcgtcg tcgcgatctc
    15781 ccaggcgacg aagctcatgg ccgtcgagcg cgtccacgtg ccctccgagc gcgtctcggt
    15841 gctgccgccc gtcgcgggac ccctccggcg gccgctcgat ccccgcgacg ccctcgcgcg
    15901 cctcgacgcc ctcgacctct tccggaccga gtacgccctc taccccgcga acttctggcc
    15961 gcacaagaac cacgagcgtc tcctcgccgc ggccgcccgc gtcgcgcgcg agacgccgga
    16021 cttcgtcctc gtcctgtgcg gcgcgctcga cggggcgcgc gagactcttc gcgccagggc
    16081 cgaggcggcc ggcctcggcg aggtcgtccg catccttccg tacctcgacg aggccgacac
    16141 gaccgccctc ctcgagggcg ccgagctgct cgtctttccg tcgctcttcg agggattcgg
    16201 gatcccggtc ctcgaggcga tggcgctgaa gacgccggtc gcctgctccg acctccccgc
    16261 tctcctcgag ctcgcgggtg acgccgcgct gttcttcgac ccgacggacg aggccgggat
    16321 cgcgcagtcg atcctccgcc tctggaccga ggaggagacg cgcgaccgcc tcgtcgcaag
    16381 aggacttgca agggccgagc gctacgcgaa agtcgacgtg gcgggcgcgt accacgagat
    16441 cctcgccgcc gccgcgagct gacggccgcc cgatacactt ccgggcgacc gtggcgcgcc
    16501 gcatcctgct tcgctcgctg gccctcgcgg ccatcctcgc ggcctgcttc gcgcacctcg
    16561 tctggaacgg cggacgcgcc gggaaggtgg tcgcggagaa cgtcccgcgt ccggccttgt
    16621 cctcgggcac gcgcgagatc gacctcctgc atcccacggc gagcttcggg cacgtctacg
    16681 acctgacgtt catgtggccc gaggaccggc cggtcaaggt gcccgagggc tcgcggctgg
    16741 aggttcagct cgcctcgaac ggccgcaccc ggcttctcgt cgtcctcccc ggtcacccgt
    16801 ccgcgagccc gtcgtggcgg gacgtccttc gaggccggca ccggcttcag ttcgtcggcg
    16861 accgcggctg gatcgacctc ggtcctctgc ccggccaggg aacgaagctg gaggtccagg
    16921 tatacggcct cggcccgggc ccgttcggct tcaccgagcc gcacgccgcg gggaccgggc
    16981 acttcagcgg ctttcagctg cgccgccccg actcgccgcg ggcccgcccg tcgctctcga
    17041 tcgtccgcta ccggttccgc gtcgcggacc ccgtccccgc aggcgagacg ctcgcccggt
    17101 tcttcttctt catctcgtgc agccgcgtcc tgcaggtcgc gggattcgtc gccctcgccc
    17161 tcctcttcgt cggctggtcc tggctctggg aggggagatt cacgagggca gtcgcggcgc
    17221 tcgttccggc ggtcacgctg ctgcacgcgt gtgggctcgc gcctctccag ggcgccgacg
    17281 aggcgacgca cgtcggcacc gtggaagccg tcctcttcaa cccgaacctg ttcgaaaacc
    17341 cggccgcgtt cccgaaatcg atcgtccagc tgtacgagag gctcggatac gagtcctggg
    17401 tcggggcacg cgaggttccg gtgacggctc tgcttccgga aaaccggccc gtcacgaggg
    17461 ccgagctttc gaggcccctc acggccgaag cggccacgga cggaggcgcg cgaatcgacg
    17521 ccgtcctgat cagcccccgt gtgcgcgcgc ccttctacta caacgcgttt cgcctcgcgg
    17581 gtccgctcct ccggggacag tccgttctcg accgggtcgc ggcctacgtg atcctgtccg
    17641 cggcggcctc gcttgcgttc ttcctcgccg gcctcctcat catgtctcgc acggggctcg
    17701 gtgctctcgc gctcgcctac ggcctcgtcg cgctctggcc gtattcggtg ggcgtcgtcg
    17761 cgagctgctc gaactactcg ctcgcgatcg gcatcgggca gttcctggcg gcatgcctcg
    17821 ttgtcggggt cctgaccccc tcgccacgct cgcggcttct ggcggcagca ctgtttctgg
    17881 cggcgagcct cttcgggatc ggcgtctggg acgacttcgt cttcgtcgcc gtcccggccg
    17941 cggccgtttt gacgattctc gccgctcatg ccgtcatccg gatgccggcc ggttccagcc
    18001 ggcggacggc gatgggcgcg ttgacggtcg ccgccgcgct cgcgaccgga acgatcctgt
    18061 tcgccctcgc gacgggtcgc gtccggcgcg ccatctccag cttcggggca cggctcccga
    18121 aagaggtcgg cggattcgaa gatccctcgt tgtggctgct tctcgccgcg gccgtcgctc
    18181 cgctgctcgt cagcctcgtc ctggctctcg gcgccgtccg cggccggatc gtttccgaag
    18241 gcggtcgccg gcgcgccgcg agggtccgtt cggcggctct cgctgcgctc ttcgtcgcga
    18301 tgttcctcgc gacgcgctgg acctcggttc cgttcgaggc cgccaggctc gactatcccg
    18361 acgaggttgc cgcccactgg tccgcgttct ggtcgaacaa cttcgcattc gaccaggacg
    18421 tgctctcgtg gaagatgtac tggggcgtct tcggatacgc ggacgtgtcg taccccgacg
    18481 ccctctacgc gctcgcgcga tgggcctgcg tggcgttctt cctcgcgctc cccctcctgt
    18541 cctggcggtt cacgctccga agcccgaacc gctcggccct cttcctcgtc gcgtgtggat
    18601 acgccctgac ggtttgcgtc gtcacgaatt cgctgcgcta cttccagccg acgaatccct
    18661 gggggcgatt catcctgccg gtcctctctc tcgtggcgct cccgctcctc gtgaggacag
    18721 acccacccga gcacgatcgc agttggcggc tcgcggtcgc ggttttcgtc gttcttcacg
    18781 tgtggacggc gaccgtgctc ctcggttccc gctacgcgct cggtctctag cccgcgttcc
    18841 cccccggccg tttctgaccc gcggccagcg gccgggccgc gccgacgagc tgcgcatccg
    18901 gcaggatctc gccgagacaa cgccggatcg catcctcgcc tccctcgtcc gcgagccggc
    18961 gcagctcctc cagcgcccgc tcgacgtctt ccggaggcga aggagagatc ttcccgatca
    19021 ggatcttcgg ccgtcccgtg ggggacacgt tctccgcatc cgttcccact tcctcgaaga
    19081 gcttctcacc cggccggatt ccggtgaaca cgacgtcgat gtcctcgttc ggcttcaggc
    19141 cggagagcgt gatcatctcg tgcgcgagat ccacgatcct gaccggttct cccatgtcca
    19201 ggacgaagat ctctccgccc ttccccatcg ccgcggcctg cagcacgagc tggctcgcct
    19261 ccgggatcgt catgaagaac cgcaccatgt cggggtgtgt gaccgtcacg ggcccgcccc
    19321 tgcgaatctg ctcgcggaaa atgggaatca cggatcccgc cgagccgagg acgttcccga
    19381 agcgcacggc gacgaatcgc gtcccgtagc gcctatcgag gtcctggacg acgatctcgg
    19441 cgacccgctt ggacgctccc atcacggacg tcggacggac ggccttgtcc gtcgagatca
    19501 tcacgaagac gtccaccccg aactccccgg cgaacgtgcc gagccgcgcg gtcccgagca
    19561 cgttgttccg gatcgcctcc gacgcgttct cttccatcag cgggacgtgt ttgtgcgccg
    19621 ccgcgtgaag gacgatcctc ggccggtaag tctcgaacac gcggcgcatc cgcgcctcgt
    19681 cgccgatgtc cgcgacgacg gcagcgggcc gggagtccgg ccagcgccgc cggatctccc
    19741 ggtcgacgtc gaaaagggcg aattcggcgc gctcgaccag cacgagctgc cgggggccga
    19801 gccgggccac ctgtcgcgcg agctccgacc cgatcgagcc gcccgcgccg gtgaccatca
    19861 cggccgcgcc gacgaggaac cgccggagct cttcttcgtc gagctggacg ggctccctac
    19921 cgagcaggtc ctcgatctga acgtcccgga tgccgctcac ctcgacgttg ccctgcagga
    19981 tctcccagat gccgggcatg atgcggactc gcacgggaat ctcctcgcag gtcttcacga
    20041 tccggcggat gtccctccgc ggggctccca cgatcgtgat gacgacctcg tcgatcgcga
    20101 gatggcggac gagtcccggc agctccgccg tggtcccgag cacgcggact ccctggatga
    20161 gcgagtcctg cttggccggg tcgtcgtcga cgaaccccct cacgttcagc tcggcgtcgc
    20221 cgcgccccag gatctcgcgc aaggccagca cgcccgcccg gccggcgccg acgaggatca
    20281 cgcgcttctg ctcggcggcg ccagccgggc gccgcccgcg ctcccagcgc tcccacgtga
    20341 agcgccgccc cacgcggaga ccgagcaccc ctccgaaccc caggaggccg tccatcacga
    20401 tgatcgagaa agggacccgg tagtcctgga gggtctcggg cagcgcgaga cgcatgacga
    20461 ggagtggaac ggtggccgcg acggccgcac gggcgaagac gagagtctcg gcgaggccca
    20521 cgtatctcca gatgaacgcg taggcgccga acgccgagag cgcggcgaac tggaggagaa
    20581 cgacgagcgg cagttgcgtc atcgcgtgcc ggaacatctc ggccgaaacc cggaagtcga
    20641 aacggatcgc gtaggcgacg aggaacgccc ccgagagcac cagtacgtcg aggaggtact
    20701 ggaccgggcg attcttgagg cgggccagga tgccgacccc gacgccgctc ggcgtccgca
    20761 ggcggcgcga ccccgtgacg gggcccgtgg aatcgtcggg acgcgtggcc atcctgcggt
    20821 tcattctagg ggctccggac gttgaccccc ggggcgcccg cgccggacaa ttgcggcttg
    20881 cactccggct gggcccgcat gctcctcgcg ctctcactgg cggctccgtg gcctcttctc
    20941 ctgggccggg gcccgaaccc ggagccgcct ccggcgcgcc cggtcttcga agaggtggcc
    21001 gcgcggagcg ggctcgattt cgtccatttc aacggggcga ccggccagga cacgctggcg
    21061 gagatcgccg gatcaggcgc cgcgctcttc gactacgacg gggacggcga tctcgacgtc
    21121 tatctggtcc agggaacgat cctcgacggc ccgctcgcga aggccgtcgt cccatggcga
    21181 ggcaagggac gccccgtcgg ccggctcttc cgcaacgacc tcgtcgtgag gcccgacggc
    21241 agccgccatc tgcgattcac cgacgtgacg gaaaaggcgg ggctcgcgtt cgaaggctac
    21301 tgcatgggag ccgcgaccgg cgacttcgac aacgacggcc gccctgacct ctacgtgacg
    21361 tgcctcggca gcaatcacct cttccgcagc cgaggggacg gcacgttcga ggacgtgacg
    21421 aagaaggccg gcgccgacga tccgcgctgg agcacgagcg cggcgttcgt cgactacgac
    21481 cgggacggct ttctcgacct gtacgtcgca aactacgtgg acttcgagac ggatccgaaa
    21541 aaggtctgcc tctctccgtc gagcgcgcgt gaatactgcg gtccgcaggc gtacgcgccg
    21601 gtcccacacc gcctgctcca caatcgtgga aacgggacct tcgaggacgt cagcgagcga
    21661 tccgggatcg ggaaggaacg gggatcgggt ctcggcgtcg tggccgccga cctcgacgga
    21721 gacggctggc ccgatctcat ggtcgcgaac gacggcaacc cgaacttcct cttcttcaac
    21781 cagcacgacg ggacgttccg caacgacgcc ctgatggcgg gcgtcgcggt gaacgcgatg
    21841 ggccgggcca tggccgggat gggcgtcgac gccggcgatt ttctcggaga cggccgggag
    21901 cacgtcttca tcacgaacat catgcaggac cccgccgcgc tcttcgtgaa cgagggcgac
    21961 gggctcttct cggaccggac gatcgcctcc ggcctcgttc ccggcacgct cggaaagacc
    22021 gggtttggaa cggggtggtt cgactgggac aacgatggct ggctggacct cgtcgtcacg
    22081 aacggggcgg ttctgaagat cccggagcag catcgggccg gcgatcctct cccgctccgc
    22141 cagcccaacg tgctgtttcg gaacacgggc tcggggacgt tcgtcgacgc gaccataatg
    22201 gccggacgcg cgttcgcgac tcccaacgtc ggaagaggga tcgcgttcgg cgacatcgac
    22261 aacgacggga cgaccgacct cctgatcacg agcaacggcg gcccggctcg cctgttcctg
    22321 aaccgcgccg gcttgcgcaa ccgctgggtc ggactgaggc tgctcgcccg ctcgggccgc
    22381 gacgcgctgg gggcgcgcgt ggaagtccgc cgcccggggc tcccgtcgct ctggaggagg
    22441 gcgcacacgg acggaagcta tctctgcgcg agcgatccgc gagttctcgt cgggctcggc
    22501 ccgactggca cgccgagagg cctccacgtg atctggccgg acgggacctc cgagacctgg
    22561 cccgcgcctc cgacaggacg ctacgtgacg atccggcagg gccgctcgcc cgccttcgaa
    22621 tagcaaatgc cccgcgggat ctcttccttc ctcctcctcg cttccctttt cttcgcggcc
    22681 gcgggcgcac gaggagagcg gagacccttc ctcgagaccg ttccgtttcc gccgggctcc
    22741 gcactcgagc ctgcagtcgc ggcacagatc catggcgtcg agaagaacct cgccgcgctc
    22801 cgcacggccg acggagtgag ccccgacgcc ctggccgagg cgtacgccga cctcgggcag
    22861 ctttttcacg cctacgagct gtgggactcc gccgcggcct cgtatcggaa cgcgatccgc
    22921 ctcgcgccga gagacatccg atcgatccat ctcctggccg aagtggcgat gaagacgggt
    22981 gacctcgagg aggcgcggcg gcggcacgcg caggtgctcg cgctccagcc ggccgatctc
    23041 gcgtcgcggg tcggcctcgg agaggccctt ctcggcctga accggcccca ggacgcccgc
    23101 cgcgagttcc tcgacgcgac acgaatcagc cccggctccg ccgccgctca tgccgggctg
    23161 gggcgcacgg ctctcgtcca gcgcgacttc tcggaggcgc gcacccgaat cgagatcgcc
    23221 cttcgactcg cgcccgaggc gacccgtctc cactacgagc tcgccatggc ctggcgcggc
    23281 ctcggaaacc gcgacgaggc cgcccgcgag ctcgcccgcg ccggcaccgt cggcgtgagg
    23341 gtcgctgatc ccctcctcga ggagctctcc gcgctgcggc gcggcgagcg cacccacctc
    23401 gtgcgggggc ggctcgcgtt cgtgaacggc cggatgcgcg aagccgccga cgagttcgcc
    23461 gccgccgtcg aggcagaacc tcgcggcatc gcgggtctcg tgggtctcgg ctcggcgctc
    23521 gcgatgctcg gagatactga ccgggccctc gacgctttcg accgcgcgct ggcgatcgcg
    23581 cccgcaaacg tcgccgccag ctacaacgtc ggccgcgttc ttctcgcgcg cggcgacttc
    23641 gccggcgcct cacgacacct gaggcgcgtc gtcgaagccg agccggaggg agcggacggc
    23701 gtccgcctcc tcgcgcggtc gctcgccgct cagggagaca ccgccgaggc cctgcgcctg
    23761 tatgagaccg cagtccggct cgcgccgcag gacgaggact ctgcgctcgg gcatgcggac
    23821 gtgctcgcgc gactgggccg attcgcggat gcgcgggacg ccctggagga cgggcaccgg
    23881 aggatgccgg accggggccg gctcgcgctc gctctcgcct accttctggc ggcgagtccc
    23941 gagacatcgg tccgcgacgg acgtcgggcg ctcgagctcg cacaggtcgt ctacgccgcg
    24001 tctccggctc ccgactccgg gcgaacggtc gcgctggcgc tcgcggagct cggacgctgc
    24061 gccgaatcgg agaagtggct cgaccgcctt ctcggcacat tcgggatgga cggcgccgca
    24121 gacatgtcgg ccctccgggc cgaccgtgat cgcgcggccc ggggcgcacc ctgccggccg
    24181 cccggacgcc cgcctcgcgc ggcgccctga gcgc
//
LOCUS       DAOMFY010000030        23623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_688_length_23623_cov_7.715547, whole genome shotgun sequence.
ACCESSION   DAOMFY010000030 DAOMFY010000000
VERSION     DAOMFY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23623
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_688_length_23623_cov_7.715547"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1183)
                     /locus_tag="PLB01_04460"
     CDS             complement(23..1183)
                     /locus_tag="PLB01_04460"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HQQ76584.1"
                     /translation="MARWERKEIALRTTATGHVLTAPVFRCHGRSERPLAYIQANVHG
                     GELQGNAAILALFDLLEKESPRGSIVLVPRVNPVSANQQVGDYVAGVYDFGSGHNFNR
                     GYVNLTGPSRGSSACYVDVDAFAAVHRDATLAEIRDGFREALRGALGAVREGSGPWGI
                     ENRLEQALAIQELALDADVVLDLHTGDRAPRYLYAPAGATAATRAFGIPFVLEVPPQF
                     GGALDEAAFVPWNDLSEAFRRLNRTDVRRFVDGFTVELGSMNAFSMAQGAEDARRIAS
                     ALRYYGVLDGEPDEPPYRIVCCNVGDYRSIYAPAAGLVDQAISPGTPVRKGDLLARMV
                     DPSRCTAMPPRSADAVVEVRAPEDGVVILFHAFASIPKGARLLSMMTNVRQL"
     gene            1241..2053
                     /locus_tag="PLB01_04465"
     CDS             1241..2053
                     /locus_tag="PLB01_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQQ76585.1"
                     /translation="MRSIPSTGEWLTPVGLGTWETFDVGPGAEMRRPLAEVLALFAAR
                     GGRLVDTSPMYGRAEGVLGALAAEAKLLPSLFVATKVWTRGKSSGLEQMERSRRLLGK
                     VDLMQVHNLLDLDTQLASIRAWKSEGRVRYTGVTHYTAAAHGELARVLKKERTDFLQV
                     NYSAAEPEAGEALLPLAADLGVAVIANRPFGGGGALRRALKTPLPGWAAEAGCASWAQ
                     IFLKWILADPAVTCAIPATSKPAHLEDNLAAASGPLPNAAWRRRAREAIAAL"
     gene            complement(2050..2595)
                     /locus_tag="PLB01_04470"
     CDS             complement(2050..2595)
                     /locus_tag="PLB01_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQQ76586.1"
                     /translation="MRVVTVNVGLPRDVAFGERRMRTAILKEPTNGAVPLRRLGFEGD
                     AQADLTVHGGPDKAAYLYPLEHYAYWADVLGRKDLAPGAFGENLTTEGLLETDVRIGD
                     VYEIGTARVLVTQPRTPCAKLGAKFGSMTFVRTFATAGRPGFYLRVLQEGMVEAGDAI
                     ARVSTDPRRPTVAEVYDGASG"
     gene            complement(2592..3560)
                     /locus_tag="PLB01_04475"
     CDS             complement(2592..3560)
                     /locus_tag="PLB01_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76587.1"
                     /translation="MRKRDGKLQGRPPLRPRLRSSIARILLIALAPFALDGCVSIGVS
                     RADLPAPPDPGAPPSGAVTVAIYERPADREADRHATFPVLSELVRLEKKGETLVARSM
                     NATWTVQDLPPGPYRLRVTKRIDDLGNVVALENPGDKAFDVLAGERAVVSVVLKKVPV
                     FWIVVLALTVVALVVLAIIGVHKGDLPKPPPLPPLPPVAIVIPLGSQGGEGGTAAPAP
                     AAVDVFPAPGSVVAARRVTVTFLLSMPLVTGGIEDHAVLAVGTSSGDVPGATSYLPDE
                     QLLTFTPSQDFVAGEEVTVTLDLSKLRGETGRTGSGRFSTSFKVAP"
     gene            3608..4081
                     /locus_tag="PLB01_04480"
     CDS             3608..4081
                     /locus_tag="PLB01_04480"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HQQ76588.1"
                     /translation="MAATPKDLRAWWTRFRDLPGGPWLFSRIFGLAIPYSGSVRPQIR
                     VLQPGRAVISMRDRRGVRNHLTSVHAIALTNLAEMTTGLALGYGLPDGMRTILVRVEC
                     EFLRKARGTITAACDAPVFTEIVEKEVLVESVLTNEAGDVVAKGRAVWLAGPVKA"
     gene            4078..5049
                     /locus_tag="PLB01_04485"
     CDS             4078..5049
                     /locus_tag="PLB01_04485"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQQ76589.1"
                     /translation="MKTALTEALGIEVPLICGAMYPCSNPELVAAVSEAGGIGVVQPL
                     SLVYVHKREFRAGMREIRALTQKPVGLNVLTEKSMGSVYAKRMSDYVDVALEEGVRFF
                     VTALGNPRWVVDRVKSAGGVVYHDVIDRRWAEKAAAEGVDGLICVNRRAGGHLGKKSP
                     EELYAEIADLKLPLVCAGGVGSPKEFRAMLDLGYAGVQCGTRFIAATECTAHADYKNA
                     ILEADEEDIVPTERVTGVPLAVINTPFIERMGTKAGPIGRLMLKGRTTKHWIRAWYTL
                     TSFYRLKGASMKGATSADCWQAGKSVATISSVQPAGEIVRSFAAALD"
     gene            5174..14701
                     /locus_tag="PLB01_04490"
     CDS             5174..14701
                     /locus_tag="PLB01_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HQQ76590.1"
                     /translation="MSAGLCSGPALASTTINHQFTPATINPGDVSQYRITIANSSLVP
                     LTAAAVTEVLPAAITIASPANITNTCGFTVSAATPGTSTVFLTAGTIPAGTGVVDGQC
                     FFQLDVTATTTGNHVATIPANTTPNATTSGYTAKESGVDVFNTTPANATLSVNTLNNP
                     TGSKAFAPSPAIAGDPVTLTITLTNPNAGATIPLTSFTDTFPAGMVVANPAGASTSCS
                     GTGFVNGSLSPAPAPNDTSVTLAGGTIGRSGTCTITVKVVVPALVPTSQVFTNLVGAG
                     AIGNTRGLTSPAFSQALTVNTPIGVTKSFGTLNIPAGQPSVMTLRISNASTANALDIT
                     SFTDNLTGTTLKIMDATSVPAAPNPAVVCTGTGAVNGTLTAPPNLVDQTISLVGAKAG
                     PGGNCTLTISMTSTVDGAHVNTVPANAVVNPKSFASPGPVTATLTANAQLTVAKTVTV
                     ANVAPGQWTQFTVTINNFSGGPVTGVTFKDVLPKNGTNQMTLFDAGSGFFTAPAPGCT
                     GGTFTGTDAAGVDTGAAPTAADAGLLWTGGTIVAGVGASPGVCTISIWAQLPATAPTG
                     LTFTNSIPIGTITGTGPAGGVGNVNAGSVNVVSFASGAVTKAFAPASIAQGQQSTLTI
                     TLFNRTVTPLTGVSLTDNLPAGVTLAANPAATNTCGGSLQAFPNDVVVKLTGGTVPAR
                     PVASQEATCAITVRVTGSVVGVKTNTINPVDFTNAQGVVIPAAVSANLTINTGITGAK
                     TFTPASVTSGGVSRVKLTITNASSGALTNVAVDDNTFSAGLTVANPANAATNCAGSPT
                     LVVTPGIARAQLLGATLNAGAACDFSFDVVTSGAGPWSDTVPVGKITSAEGASNTAAV
                     TANLTVAAAQININKSFNPVVVTGGQPSILQIDVINPSASTITGVGFTDTFPVGIVVY
                     SVPAAGTTCAGGIVTALAGDGKVRLTGATLAAGQTCQVTVTTTSTRFLNLTNTIPAGA
                     VVSNQGYTNPTGTSATLSTLQGLNVLKAFSPAWVAVGQVSTLQMSLVSSFDPGAPTPL
                     TLHGVTYTDTLPNGVFVAATPNATTTCAGTGPGGLAVVNTANGGSSGSVTFTQGTIAP
                     GTNCSITVDVVSNTLGAYNNTIPANSVTTDEGIPNQTAANATLFVVTKPTVSKAFANP
                     TRNPGQTTVLTVTITNGSGVPLTGVSLVDTLPAGVAIASTPATGGTCATTGGGVVAAT
                     AGGGTLSVTGATIAAGTSCTFFATVVSNTPGVYINNIPASSIVTNEGLSNTGPAQATL
                     TVNAPPTVAKAFNPVSIAAGGTSTLTLTLGNTNASAITLSALFTDALPGNVFVAAIPN
                     VVKTCTGGVTAAVGAAGITYANAAPIPAGGCTISVDVTSLVPGVYTNVVAAGQLSTSA
                     GVNQAPASASLAVGPGALAPPTIAKGFSPSTIFTGDTSALTIALGNPNGSALTLSADL
                     NDTLPAGVTVAAVPNVRGTCPGAVSAAAGLVTYANASTIPAGGCTIIVDVTSNVAGSY
                     TNTIAASALVTDGGSPTQPAVAGLVVKTLTPPTVLKSFSPGTINPGGTSTLTITLGNA
                     NASALTLTSPLTDTLPGSVSVAASPNASTTCSGGAVSTAASSVTLTGGSIQPGGCTVR
                     VDVTSNVPGGPYVNTIPANALQTSGGNNAAPATDRLFVNPFQPPSVAKSFTPAVIGSG
                     GVSKLSLTISNGNLSAATLTADLVDTFPGPGLVIATPPNLLLGSGCTAASVVANAGAT
                     TLTYKSGGALPANGGCSIQVDVTSSTVRSYVNPIAVGAVQTTVGSNTVAASATLQVLA
                     LPTIAKAFAPASIPLGSSSTLTLSLGNTNAVALTLASDFVDTLPANLVLGTPATVGGS
                     CASVNVVAAAGGPSVTYKSGASIPAGGCTITVPVTTALAGGYDNVIAVGGLVTTIGSN
                     PSGASAHLDVLSADLSITKTDGTPTAVPGTTTTYTIAVSNAGPSAVVNAPVADALPAA
                     VTSATWTCVATPGSSCGAASGSGNIASTVSLLAGGTATYTLVATISPSATGSLANTAT
                     VSVPAGVTDPNPGNNSATDTDTLAPSADLSVVKTGPATLVPGQNAAYTLTVTNGGPST
                     AVTVALSDTLPAGTTFVSLASPGGWACVTPAVGSGGTVSCSIASLAPLASGVFTLTVK
                     LSPAVLSGTSISNTATVTAATPDPVPGNNTSTSTGTASPSADLSIAKTGPATAIPGGA
                     NVVFTTVVTNNGPSDAQAVSVADPAPAGLTFVSNAGACATAFPCSLGPLAAGATSTIT
                     STYSIPSGYPAPAPIVNTATVSSPTPDPTPGNNTSTATVLVPNGVADVAVTKTVSNPA
                     PAVGTNVTFTITVSDLGPSDASGVAVTDLLPSGLAFVSAAPSQGAYTAATGVWTVGSV
                     ANGASATLAIVATVMQPGTIVNTATKTAGDQTDPNASNNSGSAVVNGPPSVADIQVQK
                     TVDNPTPTAGTNVTFTVRVLNAGPSNATGVAVTDALPAGLTFVSAAPSVGGYVPGTGV
                     WTIGNLANGASATLAITATVTANGTYVNTARKTGENELDPNTSNDSAVAGVVAGGGGP
                     LLADLGILKSDSPDPVRAGQDLTYTLVVTNRGPNDATGVTVTDPLPAGVSFVSASASQ
                     GGCAGTTTVVCLLGALPAGNSATISIVVSVSAAAVPSIANTASVTASESDPNPGDNSA
                     TAPTTVIPVADVGVLKTVSNPAPLVAQSFTFTVTATNNGPSTANGVVVTDAPPANLGF
                     VSAATSQGAYVSATGVWTVGTLANGASATLTLTVTALAPGAFTNTATKTGQTELDPNP
                     ANDSASASGGVGVVADLTIAKTHSPASFVRGATGTFSLTVSNVGTGPTNAPVTVTDTL
                     PAGLTPTAATGSGWGCTVTAPNVSCSRADVLAAGSAWPPVTVTVNVLQSAAGSITNSA
                     SVGGGGDVTPGNNTGTDVVPVGASADLAIVKTGPANGIPGNNVVYSLVVTNNGPSDAS
                     AVVVSDPTPPNLTFVSNSGACATPFPCALGTIAVGASRTITATFGIPANYTAPSPIVN
                     TATVSSATPDPNPANDTSSASTSVAADLAVVKTLASPATPGGTATFRLDVTNHGPSAA
                     TNVVVTDPLPAGLTVFSATATQGACTTGMTVTCALGTLQNGGTATIWITVNLPVRLQP
                     VRNVASVTADQFDPVTTNNSSSADLLGGADVPTLSEWALILLGVALAAAGARFLRQA"
     gene            complement(14712..16106)
                     /gene="sthA"
                     /locus_tag="PLB01_04495"
     CDS             complement(14712..16106)
                     /gene="sthA"
                     /locus_tag="PLB01_04495"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HQQ76591.1"
                     /translation="MQKFDLFVLGTGPAGQRAAVQAAKLGKKVGICEKREMVGGVCVN
                     TGTIPSKTFREAVLYLSGIAQRANYGAAYTVKDDVTMEDLLFRCNQVITREIDVIRAQ
                     MKRNGIALLGGAATFTGPHTLTVTGSAGAVEVEAENVVIAVGTKPAPPPVPVDGETVI
                     DSDGILTLKRLPKSLIVIGAGVIGVEYASMFAALGIDVTIVDKRPVLLDFVDDEIAEA
                     LSYFLRKLDCTLRLGEEVDTVHVERPGHVAATLKSGKKLGADLLLYSVGRVGATASLN
                     LAAAGLEADDRGRLKVDECYRTAVPHIYAVGDIIGFPALASTSSEQGRLASCHAFGVP
                     CMSLPHLFPYGIYSVPEISMVGKTEEELTKEAVPYETGVAHYREIARGAIVGDDIGLL
                     KILVHRETRLLLGVHILGTAATELIHIGQSFLALGGTLDVLVDTVFNYPTFAECYKVA
                     ALDAYNKLRVDAAG"
     gene            16147..16800
                     /locus_tag="PLB01_04500"
     CDS             16147..16800
                     /locus_tag="PLB01_04500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQQ76592.1"
                     /translation="MRSSHGAPVRAVLFDMDGVLVFSEDAWFAVYNETLAHFGHPAIS
                     RAAFDTIYGNGTAADRDAYMPERTVAEIDAAYARFFEARLDRIRPNPEAADVLRELRR
                     RGVATSVATNTNRPLAGRILEAQGLLGLVDAVTGADEAGAGKPDPAVVRLAASRTGAP
                     LEACVFVGDSRFDEEAARRAPVRFVGYRYGGGQRIESLGALPELAAPAGRTREPASP"
     gene            16814..17917
                     /locus_tag="PLB01_04505"
     CDS             16814..17917
                     /locus_tag="PLB01_04505"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQQ76593.1"
                     /translation="MPADWKKLTEETAHSFDPDEVLGMWSVDWRKAREAFFEDHRAEI
                     EAEPRRFRRGLLKANAVVYGLTRRLAPPRRIVFAVALVLVLLALLENTFGDRPSGLAF
                     IGLLVAVLALMFLLAMELVDKLHFKDELVMARALQAQLVPRPLPEIGCFELAGESRVA
                     NTVGGDLFAFEPLPGGRVAILFGDASGHGMAAGLVMAVTHAAFRTQLGVDASPSAMAA
                     SLNRVLCETGSSRSFFAGICGLLEADGTFTAIVAGHPAVLRLDAGGRVVERIGKGSYP
                     LGIKKTTEWTPLSTALAPGETLLFHSDGLPEARGESGGDYGDARIEEVAARKAGARPG
                     ELVGALTADLDRFLGRVPIDDDVSIAAIRRKPA"
     gene            17914..18777
                     /locus_tag="PLB01_04510"
     CDS             17914..18777
                     /locus_tag="PLB01_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQQ76594.1"
                     /translation="MTAGGPRRGALLIAAAAILWSTGGLAIKLVPLSAVGVVFWRSFV
                     AGVFLLAVFRPSRARWRHAAPSTVVVYALMILTFVSATKMTTAANAIFLQYTGPLYVL
                     ALGPFLLKEPFRKVDAAAVAVALSGMSLFFVGRLDPGALAGNLMAVASGLFFGLTILM
                     LRRDASGDAIPSVIAGNLAAAALALPFAWGHLGLDAKGALLVLFLGVVQLGISYVLFV
                     GGLKTVPAAEASLLGMLEPMFNPLWAFLGLGERPSTWAALGGAIVLAAVAGRTVFGAR
                     KGEAPTPPSPD"
     gene            18777..19307
                     /locus_tag="PLB01_04515"
     CDS             18777..19307
                     /locus_tag="PLB01_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HQQ76595.1"
                     /translation="MPRFVAFLRAVNVGGRVVKMDALRKIFESMGLADVESFIASGNV
                     VFSSKGVKGLDVKIAAGLERALGYEVPTFVRTAGEIADLAAQRPFAERDVAAFPTHLV
                     GFLSKNLDAAGVERLRVLESPADRFHVRGRDFWWLSKHHQARPAITGRQLENALGEPT
                     TLRNVNTIRRMAERYK"
     gene            19327..20007
                     /locus_tag="PLB01_04520"
     CDS             19327..20007
                     /locus_tag="PLB01_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ76596.1"
                     /translation="MKTDVVLLPGLHGSTALYDAFVALAPPWARCVPVPLPTLGSQSL
                     DGLATTLEAELKRLEGFVLVAESYSGPIAVRLAARLGQKVSLLALCNPLVEMPVALPA
                     GLAASAASWRGMPATAAAMVLADGDRALGQAALDGIRALPKEVLEARLRAAFAATAEE
                     IAPLLAPPVLAILGSRDRLVSPERTKALLEGVPFASVAELDAPHLVVQTKPAEVWDAI
                     SDEFESAA"
     gene            complement(20004..21578)
                     /locus_tag="PLB01_04525"
     CDS             complement(20004..21578)
                     /locus_tag="PLB01_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ76597.1"
                     /translation="MGHSSCERRRSRASELLGRALSFAAVLLGAGCGPSSPSKNPSDS
                     LVVAIESSPLHFDPRVGTDQASWRTHDVLYDALLRKGAGGEFLPDLAESWSTEDAVTW
                     RFRLRSGVKFHDGRPCEAEDVAFTYRSLLADGFASGKKEPLRIIESIETPSPLDVVFR
                     LKRPYASFPLQLLLGILPRGTTPRDADAAPVGTGPWRFVEARADDRVVFTRFAGAVGG
                     DAKLARLVMRVVPDATTRALELLNGSLDLSLNNLPPDLLPRLAESPRLAVTIRPGSTY
                     AYLAFNFRDPVLGNAKVRRALALALDRGAIAHGLFRDTVELTETLLPPDHWARLDGLP
                     PLARDLPAARRLLDEAGFPDPGGGRPRLTVAYKTSTDETSILQATAIAAQWKEAGVET
                     RIRSNDFATFYQDVVRGNFQLFSLRWQGIVDPDHYHEVFLSTSVPPKGWNRGFYADPD
                     VDRWIEEARRTADRATRKALYAEIQRRAAESLPYVSLYTMKAVAVHARDLTGLDTMHE
                     TADFTFLRNVGRTQAP"
     gene            21577..>23623
                     /locus_tag="PLB01_04530"
     CDS             21577..>23623
                     /locus_tag="PLB01_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQQ76598.1"
                     /translation="MPFKTPQSSVRPAGLAALAALLPIVSLLAAPHADAAAPRVVVLG
                     FDGADHALVKKLIDEGKLPNLAALAKKGGFSKLLPTIPAQTPVSWSTFSTGLSPGRTM
                     IFDFLKRNPKTYRPEFAIAEEGKKDFLLGKMNRFAVPAVLSLLAFVLVAGVLKILLRR
                     RVKGIGAGIVVAACVAAALFTFIGIRISSLLPTQIPFATNNRRGTPFWELAGKKGIHS
                     VVMHVPVTFPAVDYDNGRLLSGLGVPDVRGRIGTPSFYTSDPFFAPKNKNEFSVELVR
                     LESNKGTIETEVFGPYNKLFPEPPVIKTPMTLTVAADGGSLTVAPKGSDAVTLKVGEW
                     SGWVVFKFPFNSLVTMTGIGRFHLAAIAPEIKLYLSPIHFNPSDLPPSVKITAPGALA
                     KRLASRYGAYKTMGWQIDTWSMSEETIDEGAFLEDVAQTIVPFRKMMNDFLDEKEVGL
                     FVQIYEFPDRVGHCFWRFLDSGHPAYDAEKAAKWAPFMEKTYEQMDGLVGDAMKKLAP
                     EDVLLVLSDHGFATWRRSVNYNTWLVENGFMVLTGSDGKQADLEALFGQGEFWPNVDW
                     SKTRAYAMGLGDIYVNLKGREGKGIVAPGAEYEAVREEIKRKLATLVDPKTGQRAVSR
                     IFTREEAYGSFDADVIPDLFVTNAIGYRIGWQGSLGVVTKEMFEDNAQVWSGDHCSLD
                     "
BASE COUNT         3518 a         8435 c         8250 g         3420 t
ORIGIN      
        1 tccggagcct cggctacctc cgctagagct gcctcacgtt cgtcatcatc gagagcagcc
       61 gtgcgccctt cgggatggac gcgaacgcgt ggaagaggat cacgacgccg tcctccggcg
      121 cgcgcacctc gacgacggcg tccgcggagc gcggcggcat cgccgtgcag cgtgacgggt
      181 cgaccatccg ggcgaggagg tcgcccttgc ggacgggcgt cccgggggag atcgcctggt
      241 ccacgagacc ggcggcgggc gcgtagatcg aacggtagtc gcccacgttg cagcagacga
      301 tccggtaggg cggctcgtcg ggctcgccgt cgaggacccc gtagtagcga agcgccgacg
      361 cgatccgccg cgcgtcctcg gctccctgcg ccatggagaa cgcattcatc gagccgagct
      421 cgacggtgaa gccgtcgacg aaacggcgga cgtccgtgcg gttcagccgg cggaaggcct
      481 ccgagaggtc gttccacggg acgaaggcgg cctcgtcgag cgcgccgccg aactggggcg
      541 gcacctcgag gacgaagggg atcccgaacg cccgcgtcgc ggccgtcgcg cccgcgggcg
      601 cgtagaggta gcgcggcgcg cggtcgcccg tatgcaggtc gaggacgacg tcggcatcga
      661 gggccagctc ctggatcgcg agcgcctgct cgaggcggtt ctcgatgccc caggggccgg
      721 agccttcgcg cacggctccg agggcgccgc gcagggcctc gcgaaagccg tcgcggatct
      781 cggcgagcgt cgcgtcgcgg tggaccgccg cgaaggcgtc gacgtcgacg tagcaggccg
      841 agctgccccg cgagggaccc gtgaggttca cgtagccgcg attgaagttg tggccggacc
      901 cgaagtcgta gacgcccgcg acgtagtcgc cgacctgctg gttcgccgag accgggttca
      961 cgcgcgggac gaggacgatg ctgccgcgcg ggctctcctt ctcgaggagg tcgaaaaggg
     1021 cgaggatggc cgcgttgccc tgcaactcgc cgccgtggac gttcgcctgg atgtacgcga
     1081 gcgggcgctc gctgcggccg tggcaccgga agaccggcgc cgtgagcacg tgccccgtcg
     1141 ccgtcgtccg gagagcgatt tccttacgtt cccagcgcgc catgtcgact ccggacgaag
     1201 tctacaggcg cggcgcgccc gccgctaaga tcgcgtcgtg atgcggtcga tcccttcgac
     1261 gggcgagtgg ctgaccccgg tcggcctcgg gacgtgggag acgttcgacg tcggccccgg
     1321 agccgagatg cggcggcccc tggccgaggt cctcgccctc ttcgccgcgc ggggcgggcg
     1381 cctcgtggac acgtcgccga tgtacgggcg cgccgagggc gtgctcggag cgctcgcggc
     1441 cgaggcgaag ctcctgccat cgctcttcgt cgcgacgaag gtctggacgc gggggaagag
     1501 ctccggcctc gagcagatgg agcgctcgcg gcgcctgctc gggaaggtgg acctcatgca
     1561 ggtccacaac ctcctcgacc tcgacacgca gctcgcgtcg atccgcgcgt ggaagtccga
     1621 ggggcgcgtg cgctacaccg gcgtcacgca ctacaccgcg gccgcgcacg gcgagctcgc
     1681 acgcgtcctc aagaaggaaa gaacggactt cctccaggtc aactactcgg ccgccgagcc
     1741 cgaagccggg gaagccctgc tgccgctcgc ggcggacctg ggcgtcgccg tcatcgcgaa
     1801 ccggcccttc ggcggcggcg gggccctgcg ccgcgcgctg aagacgccgc tgcccggatg
     1861 ggccgccgag gcggggtgcg cgtcgtgggc gcagatcttc ctgaagtgga tcctcgcgga
     1921 cccggccgtc acgtgcgcga tcccggccac gtcgaagccg gcgcacctcg aggacaacct
     1981 cgccgcggcc tccgggccgc tgccgaacgc cgcgtggcgc aggcgcgccc gcgaggcgat
     2041 cgccgctctc tagcccgagg ccccgtcgta aacctcggcg accgtcggcc gtctcggatc
     2101 ggtcgagacc cgggcgatcg cgtcgcccgc ctcgaccatc ccctcctgga ggacgcgcag
     2161 gtagaagccg ggccgcccgg ccgtcgcgaa cgtcctcacg aacgtcatgg acccgaactt
     2221 cgcgccgagc ttcgcgcacg gcgtccgcgg ctgcgtgacg aggacgcgcg ccgtcccgat
     2281 ctcgtagacg tccccgatcc ggacgtccgt ctcgaggagg ccctccgtcg tgaggttctc
     2341 gccgaacgcg ccgggcgcga ggtccttccg tccgaggacg tccgcccagt aggcgtagtg
     2401 ttcgagcggg tagaggtagg ccgccttgtc ggggccgccg tgcacggtca ggtccgcctg
     2461 cgcgtcgccc tcgaatccga ggcgccggag cgggaccgcg ccgttcgtcg gctccttgag
     2521 gatggccgtg cgcatccggc gctcgccgaa cgcgacgtcg cgcgggaggc cgacgttcac
     2581 cgtgacaacc ctcatggcgc gaccttgaac gacgtcgaga accggccgct gcccgtgcgg
     2641 ccggtctcgc cgcggagctt cgagaggtcc agcgtcaccg tgacctcctc gccggcgacg
     2701 aagtcctgcg acggcgtgaa ggtgagaagc tgctcgtccg ggaggtagga cgtcgcgccc
     2761 gggacgtcgc cggaggacgt cccgacggcg aggacggcgt ggtcctcgat gcctccggtc
     2821 acgagcggca tcgagagaag gaacgtcacg gtgacgcgcc gcgcggcgac gacggatccc
     2881 ggggcgggga agacgtcgac ggcggcgggc gccggcgcgg cggtcccgcc ctcgcccccc
     2941 tgcgatccga gaggaatcac gatcgcgacg ggcgggagcg gcggcagcgg cgggggcttg
     3001 gggaggtcgc ccttgtggac gccgatgatc gcgaggacca cgagggcgac gaccgtgagc
     3061 gccaggacga cgatccagaa gacgggcacc ttcttgagga cgacggagac gacggcgcgc
     3121 tcgcccgcga gcacgtcgaa ggccttgtcg ccggggtttt cgagcgcgac gacgtttccg
     3181 aggtcgtcga tccgcttcgt gacgcggagg cggtaggggc cgggcggcag gtcctgtacg
     3241 gtccacgtcg cgttcatcga gcgtgcgacc agcgtttcgc ccttcttctc caggcggacg
     3301 agctccgaca ggacagggaa ggtggcgtgg cggtcggctt cgcggtcggc cggcctctcg
     3361 tagatggcga cggtgacggc gcccgagggc ggagcgccgg gatcgggagg ggccggaagg
     3421 tcggcccgcg agactccgat cgagacgcag ccgtcgagcg cgaagggcgc aagggcgatg
     3481 agaaggattc gcgcgatcga ggaacgaagg cggggtcgca ggggtggcct cccctgaagc
     3541 ttgccatccc tcttcctcat tcgaagaaaa ttcttcttcc tcttcttctc ctagaatccc
     3601 ggcccggatg gccgcgaccc cgaaggatct gcgcgcgtgg tggacccggt ttcgcgacct
     3661 gccgggcggg ccgtggctct tctcgcgcat cttcggcctt gcgatcccat actcggggag
     3721 cgtgagaccg cagatccgcg tgctccagcc cggccgcgcc gtgatttcca tgcgcgaccg
     3781 ccgcggcgtg cgcaatcacc tgacatccgt ccacgcgatc gccctcacga acctcgcgga
     3841 gatgacgacg gggctcgcgc tgggctacgg cctgcccgac gggatgcgga cgatcctcgt
     3901 ccgcgtcgaa tgcgaatttc tcaggaaggc gcgcggcacg atcaccgccg cgtgcgacgc
     3961 gccggtcttc acggagatcg tcgagaagga ggtcctcgtg gagagcgtcc tcacgaacga
     4021 ggcgggcgac gtcgtcgcga aggggcgggc cgtgtggctc gcgggtccgg tgaaggcgtg
     4081 aagacggcgc tgaccgaggc cctcgggatc gaggtcccgc tcatctgcgg cgcgatgtac
     4141 ccctgctcga accccgagct cgtcgcggcc gtctcggagg cgggcgggat cggcgtcgtt
     4201 cagccgctct ccctcgtcta cgtgcacaag cgcgagttcc gcgcggggat gcgcgagatc
     4261 cgggccctca cgcagaagcc tgtcgggctc aacgttctga cggagaagtc gatggggtcc
     4321 gtctacgcca agcggatgtc ggactacgtg gacgtggcgc tcgaggaggg ggtgcgcttt
     4381 ttcgtgacgg cgctcggaaa cccgcgctgg gtcgtggacc gcgtgaagag cgcgggcggc
     4441 gtcgtctacc acgacgtgat cgaccggcgc tgggccgaaa aggcggccgc cgagggcgtg
     4501 gacggcctca tctgcgtgaa ccgccgggcc ggggggcacc tcgggaaaaa gagccccgaa
     4561 gagctctacg ccgagatcgc ggacctgaag cttccgctcg tgtgcgccgg cggcgtcggc
     4621 agcccgaagg agttccgcgc catgctcgac ctcgggtacg cgggcgtcca gtgcggcacg
     4681 cgcttcatcg cggccaccga gtgcacggcg cacgcggact acaagaacgc gattctcgaa
     4741 gcggacgagg aggacatcgt cccgaccgag cgtgtcacgg gcgtccctct cgcggtcatc
     4801 aacacgcctt tcatcgagcg gatggggacg aaggcgggcc cgatcggccg gctcatgctc
     4861 aagggccgga cgacgaagca ctggatccgc gcgtggtaca cgctcacgtc gttctaccgg
     4921 ctcaagggcg cgtcgatgaa gggcgcgacg tcggccgact gctggcaggc cgggaagagc
     4981 gtcgcgacga tctcgtccgt ccagccggcg ggggagatcg tgaggagctt cgcggcggcg
     5041 ctcgattgac cccgcggtct gatgcaagtt ggtgcttgag tcccggcgtc gggtcggata
     5101 gcgtcgcggc tcgtccgtga gaatcctgcg tacgtcgatt gaacgtccgg tatggctcct
     5161 tcgtgcgctc ctcgtgagcg ccgggctgtg ctccggcccg gcgctggcgt ccacgaccat
     5221 caaccaccag ttcacgcccg cgacgatcaa tcccggcgac gtctcccagt accggatcac
     5281 gatcgcgaac agctcgctgg tcccgctgac tgcggcggcc gtcacggagg tcctgccggc
     5341 cgcgatcacg atcgcgagcc cggcgaacat cacgaacacg tgcggattca cggtgagcgc
     5401 ggcgaccccg ggcacgtcca cggtgttcct gacggcgggg acgatcccgg cgggcaccgg
     5461 tgtcgtggat gggcagtgtt tcttccagct cgacgtgacg gcgacgacca cgggcaacca
     5521 cgtcgccacg atcccggcga acacgacgcc gaacgcgacg acgagcggct acaccgcgaa
     5581 ggagagcggc gtcgacgtct tcaacacgac acccgcgaac gccacgcttt ccgtcaacac
     5641 gctcaacaac ccgacgggaa gcaaggcctt cgcgccgtcg cccgcgatcg cgggcgatcc
     5701 agtgaccctc acgatcacgc tcacgaaccc gaacgccggc gcgacgatcc cgctcacgag
     5761 cttcaccgac acgttcccgg cgggcatggt cgtcgcgaac cccgcgggcg cctccacgag
     5821 ctgctcgggc acgggcttcg tgaacggcag cctctcgccc gcgcccgccc cgaacgacac
     5881 gtcggtgacg ctcgccggcg gcacgatcgg gcgcagcggc acgtgcacga tcacggtgaa
     5941 ggtggtcgtt ccggcgctcg tgccgacgtc ccaggtgttc acgaacctgg tcggcgccgg
     6001 cgcgatcggg aacacccggg gcctcacgag cccggcattc agccaggccc tcaccgtcaa
     6061 cactccgatc ggcgtcacga agagcttcgg cacactgaac atcccggccg gacagccctc
     6121 ggtcatgacg ctacggatca gcaacgcgag caccgcgaac gcgctcgaca tcacgagctt
     6181 cacggacaac ctcactggca cgacgctgaa gatcatggac gcgacgagcg ttcccgccgc
     6241 gccgaacccg gccgtcgtct gcaccgggac gggagcggtg aacgggacgc tgacggctcc
     6301 gccgaacctc gtcgaccaga cgatctcgct cgtcggagcg aaggccggcc ccggcgggaa
     6361 ctgcacgctc acgatctcca tgacgtcgac ggtggacggc gcccacgtga acaccgtgcc
     6421 ggcgaatgcc gttgtgaacc cgaagagctt cgcgagcccc gggcctgtca ccgcgacgct
     6481 cacggcgaac gcgcagctca cggtcgccaa gacggtcacg gtggcgaacg tggcgccggg
     6541 gcagtggacg cagttcacgg tgacgatcaa caacttctcg ggtgggccgg tcaccggtgt
     6601 gacgttcaag gacgtcctcc cgaagaacgg cacgaaccag atgacgctct tcgacgccgg
     6661 gagcggcttc ttcacggctc cggcgccggg ctgcaccggc gggacgttca cgggcacgga
     6721 cgccgcgggc gtcgacacgg gagcggcgcc cacggccgcg gacgcgggcc tcctctggac
     6781 gggcggcacg atcgtcgccg gcgtaggcgc gagcccggga gtgtgcacga tcagcatctg
     6841 ggcccagctg ccggcgaccg cgccgacggg cctgaccttc acgaactcga ttccgatcgg
     6901 cacgatcacg ggcacggggc ccgcaggcgg cgtggggaac gtcaacgcgg gcagcgtgaa
     6961 cgtcgtctcg ttcgcgtccg gcgcggtcac gaaggcgttc gcgccggcgt cgatcgcgca
     7021 gggccagcag tcgacgctca cgatcacgct cttcaaccgg accgtgacac cgctgacggg
     7081 cgtctccctg accgacaacc tgccggcggg cgtcacgctc gcggcaaacc cggcggcgac
     7141 gaacacgtgc ggtggctcgc tgcaggcctt cccgaacgac gtcgtcgtga agctgaccgg
     7201 agggacggtg ccggcgcggc ccgtcgcctc gcaggaggcg acctgtgcga tcacggtgag
     7261 ggtgacgggg tcggtggtgg gcgtgaagac gaacacgatc aaccctgtgg acttcacgaa
     7321 cgcgcagggt gtcgtgattc ccgcggcggt ctcggccaac ctcaccatca acacgggcat
     7381 cacgggggcg aagaccttca cgcctgcttc cgtcacgtcc ggtggcgtct cccgcgtgaa
     7441 gctgacgatc acgaatgcgt ccagcggcgc cctgacgaac gtcgccgtgg acgacaacac
     7501 gttcagcgcc ggcctgaccg tcgcgaaccc ggcgaacgcg gccacgaact gcgccggctc
     7561 gccgacgctg gtcgtcaccc cgggaatcgc gcgggcgcag ctcctcggcg cgacgctcaa
     7621 tgccggcgcg gcctgcgact tctcgttcga cgtcgtcacg agcggcgcgg gcccgtggag
     7681 cgacaccgtt ccggtcggaa agatcacgag cgccgagggc gcatccaaca cggctgccgt
     7741 caccgcgaac ctgacggtcg cggccgccca gatcaacatc aacaagtcgt tcaatccggt
     7801 cgtcgtgacg ggcgggcagc cgtccatcct ccagatcgac gtcatcaacc cctcggcctc
     7861 gacgatcacg ggcgtcgggt tcacggacac gttcccggtc ggcatcgtgg tctattccgt
     7921 gccggccgcc ggcaccacgt gcgcgggcgg gatcgtgacc gcgctcgccg gggacggaaa
     7981 ggtgcgcctc accggcgcga cgctcgcggc cgggcagacg tgccaggtga ccgtgacgac
     8041 gacttcgacg cgtttcctga acctcacaaa cacgattccg gccggcgccg tcgtcagcaa
     8101 ccagggttac acgaacccga cgggaacgtc cgcgacgctg tccacgctgc agggcctgaa
     8161 cgtcctcaag gccttctcgc cggcctgggt ggcggttggg caggtctcga ccctccagat
     8221 gagcctcgtg agctcgttcg atccgggagc gccgacgccg ctcacgctcc acggcgtcac
     8281 ctacacggac acgctcccga acggcgtctt cgtcgcggca accccgaacg cgacgacgac
     8341 ctgcgccggg accggtccgg gcggcctcgc ggtcgtgaac acggccaacg gcgggtcgag
     8401 cggctcggtc acgttcacgc agggcacgat cgcgccgggc acgaactgct cgatcacggt
     8461 cgacgtcgtg tcgaacacgc tgggcgccta caacaacacg atcccggcga actccgtcac
     8521 gaccgacgag ggcattccga accagaccgc cgcgaacgcc acgctcttcg tcgtgacgaa
     8581 gcccaccgtc agcaaggcgt tcgcgaaccc gacgcggaac ccgggccaga cgacggtcct
     8641 gacggtgacg atcacgaacg gctcgggcgt tccgctcacc ggggtctcgc tcgtcgacac
     8701 gctccccgcg ggcgtcgcga tcgcgtcgac gcccgcgacg ggcggcacgt gcgcgacgac
     8761 gggcggcgga gtcgtggcgg ccaccgccgg cggcggcacg ctctccgtga cgggtgcgac
     8821 gatcgcggcc gggacgagct gcacgttctt cgcgaccgtc gtctcgaaca cgccgggcgt
     8881 ctacatcaac aacattcccg cctcgagcat cgtcacgaac gagggcctct ccaacacggg
     8941 ccccgcgcag gcgacgctca ccgtgaacgc accgccgacg gttgccaagg cgttcaatcc
     9001 ggtctcgatc gccgccggcg ggacgtccac gctgacgctg acgctcggga acacgaacgc
     9061 gagcgcgatc acgctctcgg cgctgttcac ggacgcgctg cccgggaacg tcttcgtcgc
     9121 ggcgataccg aacgtcgtga agacctgcac ggggggcgtc accgcggcgg tcggcgccgc
     9181 gggcatcacg tacgcgaacg ccgctccgat tccggccggc ggctgcacga tctcggtgga
     9241 cgtcacgtcg ttggttcccg gcgtctacac gaacgtcgtc gcggccgggc agctctccac
     9301 gagcgcgggc gtcaaccagg caccggccag cgcgagcctc gccgtcgggc ccggcgccct
     9361 cgcgcccccg acgatcgcga agggcttctc gccgtccacg atcttcacgg gcgacacgtc
     9421 cgcgctcacg atcgcgctcg ggaacccgaa cggctcggcc ctgacgcttt ccgccgacct
     9481 gaacgacacc ctcccggcgg gcgtcacggt ggcggccgtc ccgaacgtcc gcggaacgtg
     9541 ccccggcgcg gtgagcgccg cggcaggcct cgtcacgtac gcgaacgcct ccacgattcc
     9601 ggcgggcggc tgcacgatca tcgtggacgt cacgtcgaac gtcgcgggct cttacacgaa
     9661 cacgatcgct gcgtccgctc tcgtcaccga cggcggaagc cccacgcagc ccgcggtggc
     9721 cggcctcgtc gtgaagaccc tcacgcctcc cacggtgctg aagtcgttca gtcccgggac
     9781 gatcaacccg ggcggcacgt cgacgctgac gatcaccctc ggaaacgcga acgcgtcggc
     9841 gctcacgctc acgagcccgc tgaccgacac cctgccgggc agcgtctctg tcgccgcgtc
     9901 gccgaacgcg tcgacgacgt gcagcggcgg cgccgtctcg acggcggcct cgagcgtcac
     9961 gctcacgggc ggcagcatcc agccgggcgg ctgcacggtc cgcgtggacg tcacgtccaa
    10021 cgttccgggc gggccgtacg tgaacacgat cccggcgaac gcccttcaga cgagcggcgg
    10081 aaacaatgcg gcgcccgcca cggaccgcct gttcgtcaac ccgttccagc cgccgagcgt
    10141 cgcgaagtcg ttcacgccgg ccgtcatcgg ctcgggcggc gtctcgaagc tctccctcac
    10201 gatctcgaac ggcaacttgt cggccgcgac gctgacggcc gacctcgtgg acacgttccc
    10261 ggggccggga ctcgtcatcg cgacgccgcc gaatctcctc ctcggaagcg gctgcacggc
    10321 cgccagcgtg gtcgcgaacg cgggcgccac gaccctgacg tacaagagcg ggggagccct
    10381 gcccgcgaac ggcggctgct cgatccaggt cgacgtgacg tcctccaccg tgcggtcgta
    10441 cgtgaatccg attgcggttg gcgcggtcca gacgaccgtc ggcagcaaca cggtcgcggc
    10501 gtccgcgacc cttcaggtgc tcgccctgcc caccatcgcc aaggccttcg caccggcctc
    10561 gatcccgctc ggctcgagct cgaccctcac cctctcgctc ggcaacacga acgcggtcgc
    10621 attgaccctc gcgtcggatt tcgtcgacac gctgccggcg aatctcgtcc tcggcactcc
    10681 ggcgaccgtc ggcggctcgt gtgcatccgt gaacgtcgtc gcggccgccg gcggcccgag
    10741 cgtcacgtac aagagcgggg cctcgattcc cgcgggtggc tgcacgatca ccgtgcccgt
    10801 gaccacggcg ctcgcgggcg gatacgacaa cgtgatcgcc gtgggcgggc tcgtcacgac
    10861 gatcggctcg aacccgtcgg gagccagcgc ccatctcgac gtcctctcgg ccgacttgtc
    10921 gatcacgaag acggacggca cgccgacggc ggtccccggg acgacgacga cttacacgat
    10981 tgccgtctcg aacgccgggc cgagcgcggt cgtgaatgcg cccgtcgcgg acgcgcttcc
    11041 ggcggccgtc acgtcggcga cgtggacgtg cgtcgcgacc cccggatcga gctgcggcgc
    11101 cgcgagcggc agcggaaaca tcgcctcgac ggtctcgctc ctcgccggcg gcacggcgac
    11161 gtacacgctc gtcgccacga tctccccctc ggcgacgggc tcgctcgcga acacggcgac
    11221 cgtgtccgtg cccgccggcg tgaccgatcc gaacccgggc aacaactccg cgacggacac
    11281 cgacacgctc gcgccgtcgg cggacctctc ggtcgtcaag accggcccgg cgacgctcgt
    11341 cccgggccag aatgcggcat acacgctcac cgtgacgaac gggggcccgt ccacggcggt
    11401 cacggtggcc ctgtccgaca cgctgcccgc cgggacgacg ttcgtttcgc tcgcgtctcc
    11461 cggcggctgg gcgtgcgtca cgcccgccgt cggctcgggc ggaaccgtct cgtgctcgat
    11521 cgcgtcgctc gcgccgctgg cgtccggcgt cttcacgctc accgtgaaac tgagcccggc
    11581 ggtcctgtcc ggaacttcca tctcgaacac ggcgacggtg acggcggcga cgccggaccc
    11641 ggttccgggc aacaacacct cgacgtccac gggcaccgcg tccccgtccg cggacctgtc
    11701 gatcgccaag accggcccgg cgacggcgat tccgggcggc gcgaacgtcg tcttcacgac
    11761 ggtcgtcacg aacaacggtc cctccgacgc gcaggccgtg tcggtcgcgg acccggcgcc
    11821 cgcgggcctg acgttcgtct cgaacgcggg cgcgtgcgcg accgccttcc cctgcagcct
    11881 cgggccgctc gccgccgggg cgacgagcac gatcacgagc acgtattcca tcccatccgg
    11941 ctacccggct cctgcgccga tcgtcaacac ggcgaccgtc tcgtcgccga cgcccgatcc
    12001 gacgcccggg aacaacactt cgaccgcgac ggtcctcgtc ccgaacggcg tcgccgacgt
    12061 cgccgtgacg aagaccgtca gcaatccggc cccggccgtc ggcacaaacg tgacgttcac
    12121 gatcaccgtg tccgacctcg gcccgagcga cgcgtcgggg gttgccgtga cggacctcct
    12181 gccgtcgggc ctcgcgttcg tctccgcggc gccgtcgcag ggcgcctaca cggccgcgac
    12241 gggcgtctgg accgtcggga gcgtcgcgaa cggcgcgtcc gccacgcttg cgatcgtcgc
    12301 gacggtcatg cagcccggga cgatcgtgaa cacggcgacg aagacggccg gggaccagac
    12361 ggacccgaac gcgtccaaca actcgggctc ggccgtcgtg aacggtccgc cctccgtcgc
    12421 ggacatccag gtccagaaga cggtcgacaa tccgacgccg acggccggca cgaacgtcac
    12481 gttcaccgtg cgggttctca acgcgggccc ctccaacgcg accggcgtcg cggtgacgga
    12541 cgcgctgccc gccggtctca cgttcgtgag cgcggcgccc tcggtcggag gctacgtccc
    12601 gggcaccggc gtctggacga tcggcaacct cgcgaacggc gcttccgcga cacttgcgat
    12661 caccgcgacc gtgacggcca acgggacgta cgtgaacacc gcccggaaga ccggtgagaa
    12721 cgagctcgac ccgaacacgt ccaacgacag cgcggtcgcg ggcgtcgtcg ccggcggcgg
    12781 cggcccgctg ctggccgacc tcggcatcct caagtccgac tcgcccgatc cggtccgcgc
    12841 cggacaggac ctcacgtaca cgctcgtcgt gacgaaccgg gggccgaacg acgcgacggg
    12901 cgtgacggtc acggacccgc tgccggcggg tgtttccttc gtctccgcgt cggccagcca
    12961 gggcggctgc gccggcacga ccaccgtcgt ctgcctcctc ggggcgctgc ccgcgggcaa
    13021 cagcgcgacg atctcgatcg tcgtctcggt gtccgcggcg gcggtgccgt cgatcgcgaa
    13081 cacggcgagc gtcacggcga gcgagtccga cccgaacccc ggcgacaact ccgcgacggc
    13141 gcccacgacc gtcatcccgg tcgcggacgt cggcgtcctg aagaccgtgt cgaacccggc
    13201 gccgctcgtc gcgcagagct tcacgttcac cgtgacggcg acgaacaacg gcccgtcgac
    13261 ggcgaacggg gtcgtcgtga cggacgcccc gccggcgaat ctcggcttcg tctcggccgc
    13321 gacgtcgcag ggggcgtacg tgtccgcgac gggcgtctgg acggtcggca cgctcgcgaa
    13381 cggcgcctcg gccaccctca ccctcacggt cacggcgctc gcccccggcg cgttcacgaa
    13441 caccgcgacg aagaccggcc agacggagct ggacccgaac ccggcgaacg actccgcgag
    13501 cgcctcgggc ggcgtcggcg tcgtcgcgga tctcacgatc gcgaagaccc actcgccggc
    13561 ttccttcgtc aggggcgcga cggggacgtt ctcgctgacg gtctcgaacg tcggaacggg
    13621 gccgacgaac gcgcccgtga ccgtgacgga tacgctgccc gcgggcctca cgccgaccgc
    13681 cgccacgggc tccgggtggg gctgcacggt gacggcgccg aacgtctcgt gctcgcgtgc
    13741 cgacgtcctc gcggccggct cggcctggcc cccggtcacc gttaccgtga acgtgctcca
    13801 gtcggcagcg gggagcatca cgaactcggc ttcggtgggc gggggcggcg acgtgacgcc
    13861 gggaaacaac acggggacgg acgtcgtccc ggtcggcgcc tccgcggacc tcgcgatcgt
    13921 caagacgggc cccgcgaacg ggatcccggg caacaacgtc gtctactcgc tcgtcgtgac
    13981 gaacaacggc ccctcggacg cctcggccgt cgtcgtctcc gacccgacgc cgccgaacct
    14041 cacgttcgtc tcgaactccg gtgcctgcgc gacgccgttc ccgtgcgcgc tgggaacgat
    14101 cgccgtgggc gcctcgcgca cgatcacggc gacgttcggg atcccggcga actacacggc
    14161 gccgagcccg atcgtgaaca cggcgacggt gtcgtcggcc acgccggacc cgaatccggc
    14221 gaacgacacc tcgagcgcgt cgacctccgt cgcggccgac ctcgccgtcg tgaagacgct
    14281 cgcgtctccg gccacgcccg gcggcacggc gacgttccgg ctcgacgtca cgaaccacgg
    14341 cccgtccgcg gcgacgaacg tcgtcgtgac cgacccccta cccgccgggc tgacggtctt
    14401 ctcggcgacg gcgacacagg gcgcctgcac cacgggcatg acggtgacct gcgccctcgg
    14461 aaccctgcag aacggcggga ctgcgacgat ctggataacg gtcaacctcc ccgtgcggct
    14521 ccagcccgtg agaaacgtcg catccgtcac ggccgaccag ttcgaccccg tcacgacgaa
    14581 caacagctcg tcggcggacc tgctcggggg ggcggacgtc ccgaccctgt cggaatgggc
    14641 cctgatcctc ctgggggtcg cgctcgccgc cgcgggcgcc cgcttcctgc gccaggcttg
    14701 atcggtctct tctagcctgc cgcgtccacc cgcagcttgt tgtacgcgtc cagcgcggcg
    14761 accttgtagc actcggcgaa ggtcgggtag ttgaagacgg tgtccacgag gacgtcgagc
    14821 gttccgccga gcgcgaggaa gctctgcccg atgtggatga gctccgtcgc ggccgtgccg
    14881 aggatgtgga cgccgaggag gagccgcgtc tcgcggtgga cgaggatctt caggagcccg
    14941 atgtcgtcgc ccacgatggc gccccgcgcg atctcccggt agtgcgcgac gcccgtctcg
    15001 taggggaccg cctccttcgt gagctcctcc tccgtcttcc cgaccatcga gatctcgggg
    15061 acggaataga tcccgtacgg gaagaggtgg gggagcgaca tgcacgggac gccgaacgcg
    15121 tggcaggagg cgaggcgccc ctgctccgac gacgtggagg cgagcgccgg gaagccgatg
    15181 atgtcgccga cggcgtagat gtggggcacc gccgtgcggt agcactcgtc caccttcagg
    15241 cggccgcggt cgtccgcctc gaggccggcg gcggcgaggt tcagcgaggc cgtcgcgccg
    15301 acgcggccga cggagtagag caggaggtcc gccccgagct ttttgcccga cttcagcgtc
    15361 gcggcgacgt gcccgggcct ctcgacgtgc acggtgtcga cctcctcgcc gaggcggagc
    15421 gtgcagtcca gcttgcgcag gaagtacgac agagcctcgg cgatctcgtc gtcgacgaag
    15481 tcaaggagga cgggccgctt gtcgacgatc gtgacgtcga ttccgagcgc cgcgaacatc
    15541 gaggcgtact cgacgccgat gacgcccgca ccgatgacga tgagcgactt cggcagcctc
    15601 ttcagagtca ggatgccgtc ggaatcgatc acggtctcgc cgtcgacggg cacgggcggc
    15661 ggcgccggct tcgtcccgac ggcgatcacg acgttctccg cctcgacctc gacggcgcct
    15721 gccgagccgg tcacggtgag cgtgtgcggt cccgtgaacg tcgccgcccc gccgagaagc
    15781 gcgatcccgt tgcgcttcat ctgggcgcgg atgacgtcga tctctcgcgt gatgacctgg
    15841 ttgcagcgga aaagcaggtc ctccatcgtg acgtcgtcct tcacggtgta ggccgcgccg
    15901 tagttcgcgc gctgggcgat cccgctcagg tagaggacgg cttcgcggaa cgtcttggac
    15961 gggatcgtcc ccgtgttcac gcagacaccg cccaccatct cgcgcttctc gcagatcccg
    16021 accttcttgc cgagcttggc ggcctgcacg gccgcgcgct gcccggccgg acccgtcccg
    16081 aggacgaaaa ggtcgaattt ctgcatgagc ggtggattct agccgcccgt gtgaagatta
    16141 cggggagtgc gctcctctca cggggccccg gtgcgggccg tcctcttcga catggacggc
    16201 gttctcgtgt tcagcgagga cgcctggttc gccgtctaca acgagacgct cgcgcatttc
    16261 ggacacccgg cgatctcccg ggcggcgttc gacacgatct acggcaacgg cacggcagcc
    16321 gaccgcgacg cctacatgcc cgagcggacg gtcgccgaga tcgacgccgc gtacgcccgc
    16381 ttcttcgagg cccggctcga ccgcatccgc ccgaacccgg aggccgccga cgtcctccgg
    16441 gagctgcgga ggaggggcgt ggcgacgtcc gtcgcgacga acacgaaccg gcctctcgcc
    16501 ggccgcatcc tcgaagcgca gggcctcctc ggcctcgtgg acgccgtcac gggagccgac
    16561 gaggcggggg cggggaagcc ggaccccgcc gtcgtgcgcc tcgcggcctc gaggaccggc
    16621 gcgcccctcg aggcctgcgt cttcgtgggc gactcgcggt tcgacgagga ggccgcccgc
    16681 cgggctcccg tgcggttcgt ggggtaccgt tacggcgggg ggcagcggat cgagtcgctc
    16741 ggcgcgctgc cggagctcgc cgcgcccgcc gggagaaccc gcgagcccgc gagtccgtaa
    16801 gagggacaga gccatgcccg ccgactggaa gaagctcacc gaggaaaccg cccactcctt
    16861 cgacccggac gaggtcctcg ggatgtggtc ggtcgactgg cggaaggcgc gcgaggcgtt
    16921 cttcgaggac catcgcgccg agatcgaggc cgagccgcgc cgcttccggc gcgggctcct
    16981 gaaggcgaac gcggtcgtct acggcctcac gcgccgcctc gccccgcccc ggcgcatcgt
    17041 cttcgccgtc gccctcgtcc tcgtcctcct cgccctcctc gagaacacgt tcggcgaccg
    17101 gccgtcgggc ctcgcgttca tcggcctcct cgtcgcggtc ctcgcgctga tgttcctcct
    17161 cgccatggag ctcgtcgaca agcttcactt caaggacgag ctcgtgatgg cgcgcgcgct
    17221 gcaggcgcag ctcgtcccgc ggccgctgcc cgagatcggg tgcttcgagc tcgcgggcga
    17281 gagccgcgtc gcgaacacgg tcggcggcga ccttttcgcc ttcgagccgc tccccggcgg
    17341 gcgcgtcgcg atcctgttcg gagacgcgtc ggggcacggg atggcggcgg gcctcgtgat
    17401 ggccgtgacg cacgcggcct tccggacgca gctcggcgtc gacgcctctc cctccgccat
    17461 ggccgcgtcg ctcaaccggg tcctctgcga gacgggcagc tcacggtcct tcttcgcggg
    17521 gatctgcggc ctcctcgaag ccgacgggac gttcaccgcg atcgtcgcgg gccacccggc
    17581 ggtcctccgc ctggacgccg ggggccgcgt cgtcgaacgg atcggaaagg ggtcgtatcc
    17641 tctcggcatc aagaagacga ccgagtggac gcccctgtcg acggccctcg cgcccggcga
    17701 gacgctgctc ttccattccg acggcctgcc cgaggcgcgc ggcgagagcg gcggcgacta
    17761 cggcgacgcg cgcatcgagg aggtcgcggc ccgcaaggcc ggcgcgcggc ccggcgagct
    17821 cgtcggggcg ctcacggccg acctcgaccg gttcctcggc cgcgttccga tcgacgacga
    17881 cgtctcgatc gcggcgatcc gccgcaagcc ggcgtgacgg ccggcgggcc ccgccgaggc
    17941 gccctcctga tcgcggccgc ggcgatcctc tggtcgacgg gcggcctcgc gatcaagctc
    18001 gtgccgctct ccgccgtcgg cgtcgtcttc tggcgctcgt tcgtcgccgg ggtctttctc
    18061 ctcgccgtct tccgtccgtc gcgcgcgcgt tggcggcacg cggcgccctc cacggtggtc
    18121 gtctacgcgc tcatgatcct gacgttcgtg tccgcgacga agatgacgac ggccgcgaac
    18181 gcgatcttcc tgcagtacac gggtccgctc tacgtcctcg cgctcgggcc gtttctcctc
    18241 aaggagccgt tccggaaggt cgacgcggcg gcggtcgccg tcgcgctctc ggggatgtcg
    18301 ctcttcttcg tgggccggct ggaccccggg gcgctcgccg gcaacctgat ggccgtcgcg
    18361 tccgggctgt ttttcggcct cacgatcctc atgctccgcc gagacgcgtc gggcgacgcc
    18421 atcccgtccg tcatcgcggg caatctcgcg gcggccgcgc tcgcgctccc gttcgcgtgg
    18481 gggcacctgg ggctggacgc gaaaggagcc ctcctcgtcc ttttcctcgg cgtcgtccag
    18541 ctcggcatca gctacgtcct cttcgtggga gggttgaaga ccgtccccgc ggccgaggcg
    18601 tcgcttctcg gaatgctcga gccgatgttc aaccccctgt gggcgttcct cggcctcggc
    18661 gagcgcccct cgacgtgggc cgcgctcggc ggcgcgatcg tcctcgccgc cgtcgcaggt
    18721 cgaacggtct tcggggcccg gaagggtgag gcgcctacgc ctccgtcgcc ggactgatgc
    18781 cgcgcttcgt cgctttcctc cgggccgtga acgtcggcgg gcgcgtcgtg aagatggacg
    18841 cgctcaggaa gatcttcgag tcgatgggcc tcgcggacgt cgagagcttc atcgcgagcg
    18901 ggaacgtcgt tttttcttcg aagggtgtga agggcctcga cgtgaagatc gcggcggggc
    18961 tcgagagggc gctcggctac gaggttccga ccttcgtccg gacggcaggc gagatcgccg
    19021 atctcgctgc gcagcggccc ttcgcggaac gggacgtggc cgcgttcccg acccacctgg
    19081 tgggattcct ttcgaagaat ctcgacgccg caggcgtcga gcgtctccgg gtgctcgaat
    19141 ctccagccga ccgcttccac gtccgggggc gggacttctg gtggctctcg aagcaccacc
    19201 aggcccggcc cgcgatcacg ggccggcagc tcgagaacgc gctcggggag ccgacgacgc
    19261 tccggaacgt gaacacgatc cggcggatgg cggagcgcta caagtgagaa gatctccgcc
    19321 cggaacgtga agacggacgt cgtcctcctg ccggggctgc acggatcgac cgcgctctac
    19381 gacgccttcg tcgcgctcgc gccgccctgg gcgcggtgcg tgcccgtccc gcttccgacg
    19441 ctcgggagcc agtcgctcga cggcctcgcg acgacgctcg aggcggagct caagcgcctc
    19501 gagggattcg tcctcgtcgc ggagtcgtac tcgggcccga tcgccgtccg gctcgcggcg
    19561 cggcttggcc agaaggtgtc gctccttgcg ctctgcaacc cgctcgtcga gatgcccgtg
    19621 gccctgcccg ccggcctcgc cgcgtccgcg gcgagctggc gcgggatgcc ggcgacggcc
    19681 gcggcgatgg tcctcgcgga cggcgaccgg gcgctcgggc aggcagcgct cgacgggatc
    19741 cgcgccctcc cgaaggaagt gctcgaggcg aggctccgag cggcgttcgc cgcgacggcc
    19801 gaggagatcg cgccgctcct cgcgccgccg gtcctcgcga tcctcggttc gcgcgaccgc
    19861 ctcgtgtcgc cggagcggac gaaggcgctc ctcgagggcg tgccgttcgc gagcgtcgcg
    19921 gagctcgacg ccccgcacct cgtcgtccag acgaagcccg cggaggtttg ggacgcgatc
    19981 tcggacgagt tcgagtcggc ggcctagggc gcctgtgtcc ggcccacgtt cctcaggaac
    20041 gtgaagtccg ccgtctcgtg catcgtgtcg aggcccgtga ggtcccgcgc gtggaccgcg
    20101 acggccttca tcgtgtagag cgagacgtag gggagggact cggccgcgcg gcgctggatc
    20161 tcggcataga gagccttgcg cgtcgcgcgg tcggcggtgc ggcgcgcctc ctcgatccag
    20221 cggtcgacgt cggggtcggc gtagaacccg cggttccatc ccttcggcgg gaccgacgtc
    20281 gagaggaaga cctcgtggta atggtcgggg tcgacgatgc cctgccagcg cagcgagaag
    20341 agctggaagt tccccctcac gacgtcctgg tagaacgtgg cgaagtcgtt cgaccggatc
    20401 ctcgtctcga cgccggcctc cttccactgt gcggcgatcg cggtcgcctg caggatcgac
    20461 gtctcgtcgg tggacgtctt gtacgcgacc gtcagccgcg gccggccgcc gccggggtcg
    20521 gggaagcccg cctcgtcgag gaggcggcgc gcggcgggga ggtcgcgggc gagcggcggg
    20581 aggccgtcga gccgcgccca gtggtcgggc gggaggagcg tttccgtcag ctcgaccgtg
    20641 tcgcggaaga ggccgtgcgc gatcgcgccg cggtcgagcg cgagggcgag cgcccgtcgc
    20701 accttggcgt tcccgagcac ggggtcgcgg aagttgaacg cgaggtacgc gtacgtcgag
    20761 ccgggccgga tcgtgaccgc gaggcgcggg ctctccgcga ggcgcggaag gaggtcgggc
    20821 ggaaggttgt tgagcgagag gtcgagggag ccgttgagga gctcgagcgc gcgcgtcgtc
    20881 gcgtccggga cgacgcgcat cacgaggcgg gccagcttcg cgtcgccgcc cacggcgccg
    20941 gcgaagcgcg tgaagaccac gcggtcgtcc gcgcgcgcct cgacgaaccg ccaggggccc
    21001 gtcccgacgg gcgcggcgtc ggcgtcgcgc ggcgtcgtcc ctcgggggag gatgccgagg
    21061 aggagctgaa gcgggaagga cgcgtagggc cgtttcaacc ggaagacgac gtcgagaggc
    21121 gagggcgtct cgatcgactc gatgatccgg agcggctctt tcttgccgct cgcgaagccg
    21181 tccgcgagga ggctgcgata ggtgaacgcg acgtcctcgg cctcgcaggg ccggccgtcg
    21241 tggaacttca cgccgcttct caggcggaag cgccacgtga cggcgtcctc cgtcgaccag
    21301 ctctcggcga ggtccggcag gaactctccg ccggcgccct tcctgaggag cgcgtcgtag
    21361 aggacgtcgt gcgtccgcca cgaggcctgg tccgtgccga cgcgcgggtc gaagtggagg
    21421 ggagacgact cgattgcgac gaccagagaa tcggagggat tcttcgaagg tgaagagggt
    21481 ccgcagcccg cgccgagaag aacggccgcg aaggagaggg cccttccaag taattccgag
    21541 gcgcgggagc gcctgcgctc gcagctagaa tgccccatgc cgttcaagac cccgcagtct
    21601 agcgtccgcc cggccgggct cgccgccctc gccgctcttc tgccgatcgt ctcgctcctc
    21661 gccgcgccgc acgcggacgc ggccgcgccg cgcgtcgtcg tcctcgggtt cgacggcgcc
    21721 gaccacgcgc tcgtgaagaa gctgatcgac gagggcaagc tcccgaatct cgcggcgctc
    21781 gcgaagaagg gcggtttctc gaagctcctg ccgacgatcc cggcgcagac gcccgtctcg
    21841 tggtccacgt tctcgaccgg gctttccccc ggccggacga tgatcttcga cttcctgaag
    21901 cggaacccga agacctaccg ccccgagttc gccattgcgg aagaggggaa gaaggatttt
    21961 ctgctcggaa agatgaaccg gttcgccgtt ccggcggtgc tcagcctgct ggcgttcgtc
    22021 ctcgtcgccg gcgtgttgaa gattcttctt agaaggcgcg tgaagggcat cggcgcgggg
    22081 atcgtggtcg ccgcgtgtgt tgccgcggcc ctattcactt tcataggaat ccgaatctct
    22141 tctcttctcc cgacccagat tcccttcgcc acgaacaacc gccgcgggac gcctttctgg
    22201 gagctcgcgg gcaagaaggg gatccactcg gtcgtcatgc acgtaccggt gacgttcccg
    22261 gccgtggact acgacaacgg ccggctcctc tcgggcctcg gcgtgccgga cgtgcgcggg
    22321 cgcatcggaa cgccgtcgtt ctacacgtcc gatccgttct tcgcccccaa gaacaagaac
    22381 gagttctccg tcgagctcgt ccgcctcgag tcgaacaagg ggacgattga gacggaggtc
    22441 ttcggcccgt acaacaagct cttccccgag ccgcccgtca tcaagacgcc catgacgctc
    22501 actgtcgcgg cggacggcgg gagcctgacg gtcgcgccga agggctcgga cgccgtgacg
    22561 ctcaaggtcg gggagtggtc ggggtgggtc gtcttcaagt tcccgttcaa ctccctcgtc
    22621 acgatgacgg ggatcgggcg cttccacctc gccgcgatcg ccccggagat caagctctac
    22681 ctctcaccga tccacttcaa cccgtcggac ctgccgcctt cggtcaagat cacggcgccc
    22741 ggcgcgctcg cgaagaggct cgcgtctcga tacggggcct acaagacgat gggctggcag
    22801 atcgacacgt ggtccatgag cgaggagacg atcgacgagg gggccttcct cgaggacgtc
    22861 gcgcagacga tcgtgccgtt ccggaagatg atgaacgact tcctcgacga gaaggaggtc
    22921 ggtctcttcg tccagatcta cgaattcccc gaccgcgtgg ggcattgctt ctggcgcttc
    22981 ctcgactccg gacatcccgc gtacgacgcc gagaaggccg caaagtgggc gccgttcatg
    23041 gagaagacgt acgagcagat ggacggactc gtgggcgacg cgatgaagaa gctcgcgccc
    23101 gaggacgtcc ttctcgtcct ctcggaccac ggtttcgcga cgtggcgccg gtccgtgaac
    23161 tacaacacct ggctcgtcga gaacggcttc atggtcctga ccggcagcga cggcaagcag
    23221 gcggatctcg aggccctgtt cggacagggc gagttctggc cgaacgtgga ctggtcgaag
    23281 acccgggcct acgcaatggg cctcggcgac atctacgtca acctcaaggg ccgcgagggc
    23341 aagggcatcg tggcgccggg ggccgagtac gaagccgtgc gcgaggagat caagaggaag
    23401 ctcgcgacgc tcgtcgaccc gaagacggga cagcgcgccg tctcgcggat cttcacgcgc
    23461 gaggaggcct acggctcgtt cgacgcggac gtgatccccg acctcttcgt cacgaacgcg
    23521 atcggttacc gcatcggctg gcagggcagc ctcggcgtcg tcacgaagga gatgttcgag
    23581 gacaacgcgc aggtctggtc cggcgaccac tgctcgctcg acc
//
LOCUS       DAOMFY010000031        23377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_692_length_23377_cov_7.091544, whole genome shotgun sequence.
ACCESSION   DAOMFY010000031 DAOMFY010000000
VERSION     DAOMFY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_23377_cov_7.091544"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PLB01_04535"
     CDS             complement(<1..907)
                     /locus_tag="PLB01_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ76599.1"
                     /translation="MISFFLAKRYLLASRRDAQVGVVALAAFLGLLLGVAALVVSLAL
                     LSGFQTNIRNRLLAETPHLLVTPAGRGEFHDADRIAGKLAAVPGVASVSPVAKGRVWV
                     SLGSQAMPAMVVGREKQKGLVLDVAQARPIGLVAGETVTLVSSRSRLSPLGPVPIVAT
                     MTIAGVTPSATGRRAAEASMPLEDARRLFALGPDGATGYELRLTEPSRAARVGEAVQA
                     ALGPSVAVATWEEQNRALVLALRIERTVLFAAVFLIVIVAGLNLAATSAVLAATRAGD
                     AAVLSVLGASPRAVGRVFRTAGACVG"
     gene            complement(1016..2665)
                     /gene="lysS"
                     /locus_tag="PLB01_04540"
     CDS             complement(1016..2665)
                     /gene="lysS"
                     /locus_tag="PLB01_04540"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007419698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQQ76600.1"
                     /translation="MSETPDPKKTAEPKAPEADVPEQIANRRANRERIAALGRPLYPN
                     RWDSTHLVSDVVARWSSRDAAALEAEPEADRRVRVPGRILAIRKMGKAIFVDLSDGAH
                     RVQSYLKKDLLRPGDWELLEQLDLGDHVGVAGVVFRTRTGELSVKGESLDFLAKSLRP
                     LPDKWHGLTDVERKYRQRYVDLVVSPETRRVFEVRAKIVAYIRRFFDGRGFVEVETPM
                     MQTLAGGAAARPFVTHHNALDLQLFLRIAPELYLKRLVVGGFPKVYEINRNFRNEGIS
                     TQHNPEFTMLEFYTAYADARDQMALTEELFAGAAQEILGTTELPWGEETILLKAPFKR
                     VSMVDAILENAEEAEKDREKEKISANGPVPTGGRIAVTRESLSDLHLLRKTAEHFGVE
                     NVERFGESKGKLLAEIFSAVAERRLLQPTYIIDYPAEISPLSKTKPGDPSTADRFELF
                     IGRMEVANGFCELNDPDEQAEKFRAQVAERTKGDDEAMLYDEDYVEALSYGMPPTAGE
                     GVGIDRLAMLFTNSHSIRDVILFPLMRPKKEEREKEKDKDA"
     gene            complement(2703..4301)
                     /locus_tag="PLB01_04545"
     CDS             complement(2703..4301)
                     /locus_tag="PLB01_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76601.1"
                     /translation="MSDAEREPESVSRAYARLSEKFKALWMFHQFLQGVHRTSLKGAP
                     LPDVPFAPLYEQIKRIKETKGVEPEDASKAAMQRLDGELDGLHATLADDDRLIPPSTL
                     RQFFERVKAGDEELLLSILKFYYYASSLGPDEMDKVDFLLTRLGTAKDADGEIVLKPA
                     ADLEKLSGALLGLTARPPIAPSEVQAVVTLLDVLRRDLDACERFEDLTKKKTLENLRT
                     LKHRLGAAFYSPEVMEAVLASNVAFKKKFQLLYREEERRILAASRDVIAKEKDLEKDT
                     RFMSAEFREDLERFRRDKEDFERASRKHGVRPRDVKRLKESLHRLLARLDPAAADELD
                     IGSDSTAGRSAGGRSGSGVRRRKSAASGSTPVPRSVAGWRAENDRVTSETARRLLSAV
                     DLVRSGGGPSVTDPSARLENWEVRAALRVYRANHEPDSEPLTAHERLYFNGAVLRCRM
                     DEEAQKLRDLVQEVGESSASDATIAPAGKCLVRAREVDRTFREALLAAENDRAAWKQI
                     TRSRFRHLRAFAGLWLLYSSLGGE"
     gene            4367..4885
                     /gene="hpt"
                     /locus_tag="PLB01_04550"
     CDS             4367..4885
                     /gene="hpt"
                     /locus_tag="PLB01_04550"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQQ76602.1"
                     /translation="MIDHILLSEETIARRLDVLAKEIDARYVGKDLLAVGVLKGSVFF
                     LTDLLKRLNVPVAVDFMQVRSYAGTSSSGNIQLLKDLSMDVAGKDVLLFEDVVDTGLT
                     LRKILELLSSRNPRSLGIAALLKKRLPENRDISVDFLGFEIGPEFVVGYGLDLDEKYR
                     NLPHVAVWDPKK"
     gene            4882..5268
                     /locus_tag="PLB01_04555"
     CDS             4882..5268
                     /locus_tag="PLB01_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQQ76603.1"
                     /translation="MSDVKTIAVDNAPKAIGPYAQGIAAGGFLFTAGQTPLDPSTMKL
                     VEGDVAAQTTRVLDNLEAVLRGAGLTFADVVRAGVFLVDMGDFAKMNEVFAARFGSHR
                     PARTTVQVAGLPANARVEIDLVAKLH"
     gene            5308..5763
                     /locus_tag="PLB01_04560"
     CDS             5308..5763
                     /locus_tag="PLB01_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76604.1"
                     /translation="MSRSSAILVFSALLAFPALADDATPAPAATPAAAAKKKAAKKAA
                     PQPAAGSANMIVTKDAETGELRPATPAEREKLLGRQPLAAPEHRVVTLPDGSQMVELT
                     EADRSYAVATKNPDGTISRTCVHGAAEAAKAVASPAPATVAAPAPKTDR"
     gene            5775..8585
                     /locus_tag="PLB01_04565"
     CDS             5775..8585
                     /locus_tag="PLB01_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76605.1"
                     /translation="MKTHLLRATLALVLGAAALPLAAADFQIVVADGTGVGFNDPTPA
                     TPVGGNTGTTIGAQRLIVFQEAARIWGSLLPSNVTIKVNSSFSALTCSATTAVLGSAG
                     ATTVHANFANAPIANTWYNKAEADKHNGVPLGTNPAAINAQFNSELGKTGCLTGTFFY
                     LGLDNNHGSNVNLLSVLLHEFGHGLGFTSAANSSGKFIGSGTNQYPGAYDRFLYDTAA
                     GKTWDQMATDAERVTSGTNTGRLVWNGTNTTTYAKAYMSRKANLVVTAPAGSAGTYAV
                     GTADFGASPAVVSVSGPVVIATDAADGAGPLTTDGCSAITNGPALAGKIALVDRGTCA
                     FVIKAKNVQNAGAIGMIVADWDPAAGVAGMSGTDATVTIPSVRVTFDDGNRLKAALPT
                     AATIGPDPTVLAGTDATGRLLMYAPNPFESGSSVSHFDTTASPNLLMEPNISGDLPIA
                     VDATLPLFRDIGWFEGSTAIPTTWILPSSAHAQGANGAFYKTDLTVSNTGAAAANLVL
                     KFLGHDADGRTGAEVTRVVPAGQTATYADVLSSVFGVSSGFGAIRVNADTNNLRIVSQ
                     TSTPPPSGVGTFGQAVPAATGNDFATTAAPKALFSLRQDAAFRTNAVIANATEAPAHV
                     DLALYNSSGVPIGSGAADLNPLEMRQIGAVITSLGGPDGTKDAFLVVSTPTADARIAT
                     YAAVIDQNTNDPRTILPATLGTLGTNGAWVLPSSAHAQGANNAFYTTDLTIGNAGTTP
                     ATVTLKFLGHDQDGSGGPEAVRTIPANSVATYPDVLGSVFGVSSGFGAILVTSTSSNL
                     KVLSQTSTPPPSLVGTFGQSVPAAGAADFVTLAAPKTLVGLRQDSAFRTNAVIANATA
                     LPAHVDLVLRSEGGAVIGSGSADLLPYEMRQIGAVVTALGAPDGTANAALVVSTTTGG
                     ARIATYAAIIDQKTNDPRTVLP"
     gene            complement(8658..9704)
                     /locus_tag="PLB01_04570"
     CDS             complement(8658..9704)
                     /locus_tag="PLB01_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HQQ76606.1"
                     /translation="MSAQISDFRSDTVTKPTPRMRQAMAEAEVGDDVYGEDPTVRRLE
                     EKTAEVLGHEAALFVPTGSMGNQLSLHVHGASGTEVILESRCHIFHYEMAGMAALSGL
                     LPRPVEGVRGRLAPDDVATWIRPESVYYLPRTSVLALENTHNGAGGTVTPREATDPLL
                     ALAKQHGLKVHLDGARLWNAAAALGVTEASLAAGFDSVMTCFSKGLRAPVGSAVSGSK
                     DFIAEARRVRKLFGGGMRQAGVIAAAALVALEEERSRLPEDHARATKLAAGLASIRGL
                     RIDPVEVETNILLFFLEGPWGDSATVVAKLKERGVLAGAAGATSIRMVTHADVNDIDV
                     ERALRAFGEIAKGH"
     gene            complement(9712..10194)
                     /locus_tag="PLB01_04575"
     CDS             complement(9712..10194)
                     /locus_tag="PLB01_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76607.1"
                     /translation="MPLADLPLEASRVLVALGLSVFVGIPLARIFGWLTRGEEEKEKE
                     QKNFLEGEEGRERLGRARFVAAGALLLGVLLEVQVLGSSLPSRHVWRAVTIFVLLASL
                     VYSVMVVAPKARYFDERSSTFEENLRAPWHARRDAARARGSKIDFLGFLLALAALVLG
                     "
     gene            10227..10889
                     /locus_tag="PLB01_04580"
     CDS             10227..10889
                     /locus_tag="PLB01_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ76608.1"
                     /translation="MGLYLIETYPVGPLQCNCAILADPSTREAVVVDPGDEPDRILET
                     LSGAGLKAIALVHTHAHFDHVGCSGLLKRITGAPIFMHEDDRPLYQNLDEQGKAFGIH
                     LEKPGIIDKLLVHGDRIPIGSGTLHVLHTPGHTPGSLSFRMEGAEGDILFSGDTLFRR
                     SIGRTDLWGGSQDQILDSIRRELLTLPGGLRVIPGHGPDTTIAEEGRRNPFVGDPGYK
                     PA"
     gene            10911..11579
                     /locus_tag="PLB01_04585"
     CDS             10911..11579
                     /locus_tag="PLB01_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQQ76609.1"
                     /translation="MRLTYEESAGLLHKFWSGEPVPSPKTGRPMRAFFVERTFAPQVV
                     MIGTKGEHFRFDQKPRQIVFSASHIKSMVRDAKERENPVCPQDFEALYVKRIPATRFP
                     GEWDWTFCCPKCLSFGTWEYTDKKDESAIPPTKKKEEAPPFHDETIPLPKRMEMAKTG
                     DLALKLYASMAQTDCTACGYDCEGYAKAIANGTEKNLSLCVPGKEETEDVIKRLMAGT
                     YTFA"
     gene            complement(11564..11758)
                     /locus_tag="PLB01_04590"
     CDS             complement(11564..11758)
                     /locus_tag="PLB01_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQQ76610.1"
                     /translation="MLLKFRPVCICNTIRGPRVTEAIARGARTVEDVAAATGCTTGEC
                     KGERCRPVIEAMLAEAQAKV"
     gene            complement(11867..14137)
                     /locus_tag="PLB01_04595"
     CDS             complement(11867..14137)
                     /locus_tag="PLB01_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76611.1"
                     /translation="MNAELFQKARKIFDEAVELPETERAEFLRRSCGAPEVREEVEAL
                     LREAEGPAFTDGVREAISGLQDEGPGAAAGRRAGPYELIREIGRGGMGSVYLARRADE
                     AFHRDVAVKLVQAGLEAGPILARFRAERQILASLSHPNIATLLDGGTTDGGLPYFVME
                     YVDGLPIDVYCDRKGSPLDERLRLFLLVCDAVQYAHRNLVVHRDMKPSNILVTPDGVP
                     KLLDFGLARLLTPEGGDERTATEWHALTPAYASPEQVRGGPVSTLSDVYSLGVLLYVL
                     VTGKRPYGDSSEPAALLHAVLAEEPARPRSRNPDLPSDVEVVVLKALRKEPEERYGSV
                     EQFGSDVSRFLAGYPVLARHGGALYRARKFVRRHWAGLAAAAAVAAALLGGFLVANQQ
                     RRIAVRRFDDLRKLARAVMFDLHDTIAPLPGSTKAREKLVRTGLEYVDALAAGESLNP
                     DLQREIAASYGRLGAVQGGGNSNLGDAAGARESFRKAIRVLQALVDSRFATDADRLQL
                     ANLHVALGQVDDSYEQEFAEALRIEKEILARHPGDFAARLAQSATYASLSSAHAGTKD
                     FARALEERRAAHAILEELAKEKPEEGRVRRELALSCKYYGSLLQRAGDLGGALRLYEQ
                     AVAIDEERLRADANSALVRLDLSFSLGSVSACLGQMGDLPGAIAFRERATNLREEIAR
                     ADPADKWANLGLARGLARLSNLQARGGDASRARATLARALDILRSWRDKDPADSELAR
                     EVADGEAELARLTGRR"
     gene            complement(14134..14715)
                     /locus_tag="PLB01_04600"
     CDS             complement(14134..14715)
                     /locus_tag="PLB01_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HQQ76612.1"
                     /translation="MTESGERPLVSPGEVTGLLRAWSNGDRDAPERLMPLVYGELRRL
                     AASQMRKERSDHTLQPTALVHEAYVKLVDQHLAWQDRGHFFGVAARAMRQVLVDHARK
                     KHAQKRAGGFQVPLESEDAAVAPRALDLLALDQALYRLAELDARQAHLVELRVFAGLT
                     VEEIGRVVGCSPATASRDYRHAEAWLRRQMRAL"
     gene            14793..17837
                     /locus_tag="PLB01_04605"
     CDS             14793..17837
                     /locus_tag="PLB01_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76613.1"
                     /translation="MRIRTLALVGMLILEGRILAGDELPIANWPSAPYWSPTAAREAR
                     SALAAVQPLPFIALTPCRIVDTRGNAPLTGGFLPAATVRSYTVTNVCGIPPGAKALSL
                     NATVVHPTGPGFLTLYPEGGTFPPVSTLNYLGNDVIVNAAVVPLSATGGISMALGVSG
                     GDVILDTNGYYAAVPSVTSLNALSGDLTLAAGTNVTLTPSGSTLTISAAGGGGGGGTV
                     TSVGTGSGLTGGPITTTGTVSVAPLGITTGMLANNAVTSAKLANGAVGLSQIDTSQVQ
                     ARVAACPPGTTVRSVNADGTVVCRAPVVIDPMISTVDGASLAGQYSSIAIGSDGRGLI
                     SYHDGLNGDLRVAHCSDAACTSATTTTLDAAGVVGQWTSITIGSDGLGLISYYDVTNG
                     NLKVAHCSNTACTSATTSVLDSAGNVGLTSSIAIGTDGLGLISYHGATDGDLKVAHCS
                     NAACSAATITLLDTPGGVGLYTSLAIGSDGLGLISYFDNSNDDLKVAHCSNTACTNAT
                     ITTLDSAGIVGSYTSITIGADGLGLISYFDNTNNHLKVAHCSNVSCTAATMSTLDSAA
                     DVGRYTSITIGADGLGLISYADATNSRLKVAHCSNTACTSATTSIVAGPTQQTSIKVG
                     PDGLGLISYFDSVGFGSLKVARMGTGGASVNGLTGPVTISGGGGTTVTSSGNTLTITA
                     PTAPPAWGLTGNAGTTPGANFLGTTDGQPLVIQAGGGVGVNKSAPSATLDVAGTISGA
                     SSSGTGVVGSSSNPNSTGVYGLASASTGFSIGVRGDTASTTGIAIWGYSTPTAATGFP
                     VGVEGRSDSPIGVGVNGFAAAGSGVNYGIRGATNSPSGWAGYFLGNVSVIGTLSKSGG
                     SFRIDHPLDPENKYLYHSFVESPDMMNIYNGNVTTDEKGYATVELPAWFEALNRDFRY
                     QLTVIGQFAQAIVSSEIETGRFVVRTDKPAVKVSWQVTGIRKDAWAENHRIPVEEVKP
                     EGERGRLLHPKEHGRPVEDGIAWRTPLAALEGRSSPAERSVP"
     gene            17834..19981
                     /locus_tag="PLB01_04610"
     CDS             17834..19981
                     /locus_tag="PLB01_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQQ76614.1"
                     /translation="MRRAALAVALLSRALLAERPDIADQPATWTAPPYWAPPATSPED
                     ATARAALAGRQPLVGGPTALPFIALTPCRIVDTRGNAPLTGGFLPAATVRSYTVTNVC
                     GIPPGAKALSLNATVVHPTGPGFLTLYPEGGTFPPVSTLNYLGNDVIVNAAVVPLSAT
                     GGISMALGVSGGDVILDTNGYYAAVPSVTSLNALSGDLTLAAGTNVTLTPSGNTLTIA
                     ATGGGGGPPSGPAGGSLSGTYPNPGIADAAVTDAKIAGVSWGKVTGAPGFVLKAGDTM
                     TASLNLNPGNINLVTEPSTATSGSILKNGLRFLHDYGSVNTFVGLNAGNFTMTGFVNT
                     VSGNNALARNTTGSYNTASGAYALTFNTTGDSNTAIGAWALSSNTTGLENTGIGSNVL
                     GANTTGSSNTASGAYALDSNTTGYDNTASGRDALYSNTTGNANTASGRGALYSNTTGN
                     ANTAKGYGALALTTTGSQNTAVGYVSLYGNTTGFQNTALGDYAGYRSGASSGYSGGAI
                     TFVPLTTGSYNTFLGRGTGATAAGISNCTAVGIDAYCDANDQVRLGNIFVGSIGGKVG
                     WSALSDARAKRDIRDLSVGLDFVLGLRPVEYRLKVGNGRTDMGFLAQDVEALLGRGYN
                     VVTAGGDPDRTLSLRYTDLIAPLVKAIQEQQALLDGRSARIDELDRRLEARDALIDDL
                     GRRLAALEERAASRQESRGPSDRRTGPAPAPAP"
     gene            complement(19978..20490)
                     /locus_tag="PLB01_04615"
     CDS             complement(19978..20490)
                     /locus_tag="PLB01_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQQ76615.1"
                     /translation="MAILKVAKLGSPILRRRASEIEPADLKKGRLKPFFEDMMETMVE
                     YHGTGLAAPQVSTGLRVVLYEVHGEKRGKDVLEVPPTILVNPSFDVLDPAEEEDFEAC
                     LSVPFLAAKVPRPKSIRVRALDTSGKPVTYVAEGFHARVVLHESDHLDGVVYLDRVKD
                     RTSIRYTVDF"
     gene            20483..21502
                     /locus_tag="PLB01_04620"
     CDS             20483..21502
                     /locus_tag="PLB01_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HQQ76616.1"
                     /translation="MATEALYRPLRLESGIRNVFVFNPAAGKGRAARDRDAIRAALEK
                     AGAHGTFVETERPGHGITLAEDAARDGAEAVIAIGGDGSVNEVANGLLRIPEATRPIF
                     GVVPDGTGNDYGYLLGLRPGDLDGAARAIAAGATRVLDAGEVNGRFFANGVGLGFDGA
                     VAETAAKVRYLKGFPAYLWSVFKVLKSWENFELTMTCDGRTISGRALLAAVANGPRSG
                     GGFLLAPDAKADDGLFDVCRLGNLGRLEALRHLPKALDGSHTGLPWVTILRAKEVVLA
                     SDRPLTAHVDGNLLTGVAHPDPLRIRILPRALRVFAPAGAQAAKPASASEPARHAERK
                     VREPA"
     gene            complement(21531..21606)
                     /locus_tag="PLB01_04625"
     tRNA            complement(21531..21606)
                     /locus_tag="PLB01_04625"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21571..21573),aa:Phe,seq:gaa)
     gene            21776..22657
                     /locus_tag="PLB01_04630"
     CDS             21776..22657
                     /locus_tag="PLB01_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQQ76617.1"
                     /translation="MQAPPGTPPATGSAYAGAAGLDVIWAATVEQAQESARGMKDGRI
                     LFFFTDPDCGECSRMLKIIAPSTSFYSFTRDKVPVPVPISTPEGLKLAKKMRVVGVPT
                     WIVATPDLLECSRQEGVTSQQGWVNTFVAGERSWAEFRKAQAAENANPADSAAVFEVA
                     RQLFQRGAREQAEGRFRRLAADPAVAPDIREQANAYVASIDLDAGRYDEAARILDELA
                     KGAKDPRLRERAELRRADVEIARGRRDLAAYRLREFQKAHPDSPLAAEARQLLDALQG
                     KASAAPLPAAAPAAKEK"
     gene            22657..23349
                     /locus_tag="PLB01_04635"
     CDS             22657..23349
                     /locus_tag="PLB01_04635"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HQQ76618.1"
                     /translation="MSPIRSFLAAGAIALAAIPGRAPAQGIPKSSEIVAPKLVADSDR
                     LVAGKAFRLAVVADIKAGWHVNSHTPKEDFLIPTEVKVRPAPGLKFSAVGYPKQLERK
                     FAFSEQKLAVYEGRTLFVVPGAVDAAAAPGPRTITAVLSYQPCNDNQCLPPAELTSTL
                     TIEVVKSGEAKPANADVFAAPSAGGAHGSASADAGADDQLIAGAEFWPGVGYPLKKEA
                     EVHTVVAWRPTL"
BASE COUNT         3687 a         8420 c         7862 g         3408 t
ORIGIN      
        1 cgcccacgca ggcccccgcg gtccggaaca cgcggcccac ggcgcgcggc gaggcgccga
       61 ggaccgacaa cacggccgcg tcgccggcgc gcgtcgccgc gagcacggcg gacgtcgccg
      121 cgagattgag gcccgcgacg atcacgatca ggaacaccgc cgcgaagagg accgtccgct
      181 cgatccgcag cgcgaggacg agggcgcggt tctgctcttc ccacgtcgcg accgcgaccg
      241 acgggccgag ggcggcctgc accgcttccc cgacccgggc cgcgcgggag ggttccgtca
      301 ggcgcagttc gtaacccgtc gccccgtcgg ggccgagcgc aaacaggcgg cgcgcgtcct
      361 cgagcggcat cgacgcttcc gcggcccgcc ggcccgtggc ggaaggcgtg acgcccgcga
      421 tcgtcatcgt cgccacgatc ggcaccggcc cgagcggcga gaggcgcgaa cgcgaggaga
      481 cgagcgtgac cgtctcgccc gcgacgaggc cgatcggacg cgcctgcgcc acgtcgagga
      541 cgagcccctt ctgcttctcg cgccccacga ccatcgccgg catcgcctgc gagccgagac
      601 tcacccacac gcggcccttc gcgacgggcg acacggaggc gacgcccggt acggcggcca
      661 gcttcccggc gatccggtcg gcgtcgtgga actccccgcg ccccgcgggc gtcacgagga
      721 ggtgcggcgt ctcggcgaga aggcggttcc ggatgttcgt ctggaagccc gagaggagcg
      781 cgagcgacac gacgagcgcc gcgacgccga gcaggagccc gaggaacgca gcgagcgcga
      841 cgacgcccac ctgcgcgtcg cgccgcgacg cgaggaggta tctcttggca agaaagaaag
      901 agatcaagag ggagaaagga agagagaagg gaagattttc ttagaaggtg aatgcgccct
      961 cgcatcggct ccgcggcccc tcgcgaggtt tgtcgcagcc ggcctgacta cctttctaag
     1021 catctttatc tttctctttc tctctttcct ctttcttcgg tctcatcaac ggaaacagga
     1081 tcacgtcgcg gatggagtgg gagttcgtga agagcatcgc gaggcggtcg atgccgacgc
     1141 cctcccccgc cgtcggcggc atcccgtacg agagcgcctc gacgtagtct tcgtcgtaca
     1201 gcatcgcctc gtcgtcgccc ttcgtgcgct cggccacctg ggcgcggaac ttctcggcct
     1261 gctcgtccgg gtcgttcagc tcgcagaacc cgttcgcgac ctccatgcgc ccgatgaaga
     1321 gctcgaagcg gtcggcggtc gaggggtcgc cgggcttcgt cttcgagagc ggcgagatct
     1381 cggccggata gtcgatgatg taggtcggct ggagcagccg tcgctccgcg acggccgaga
     1441 agatctctgc gagtagtttc cccttcgact cgccgaacct ctcgacgttc tccaccccga
     1501 agtgttccgc cgtttttctt agaaggtgaa gatcagaaag gctctcgcgg gtgaccgcga
     1561 tgcgcccgcc ggtcggcacg ggaccattcg cagaaatctt ctctttctcc ctgtccttct
     1621 ccgcctcttc cgcgttctcc aggatcgcgt cgaccatcga gacccgcttg aagggggctt
     1681 tcagcaaaat cgtctcttcg ccccacggca gctcggtcgt gccgaggatc tcctgcgcgg
     1741 ctcccgcgaa gagctcctcg gtgagcgcca tctggtcgcg cgcgtccgcg tacgccgtgt
     1801 agaactccag catcgtgaac tcggggttgt gctgcgtcga gatgccctcg ttgcggaagt
     1861 tgcggttgat ctcgtagacc ttcgggaacc cgcccacgac gagccgcttg aggtagagct
     1921 cgggcgcgat gcgcaggaag agctgcaggt cgagcgcgtt gtggtgcgtc acgaagggcc
     1981 gcgccgccgc gccgcccgcg agcgtctgca tcatcggcgt ctcgacctcg acgaagccgc
     2041 gtccgtcgaa gaagcgccgg atgtaggcga cgatcttcgc gcgcacctcg aacaccctgc
     2101 gcgtctccgg cgagacgacg aggtccacgt agcgctggcg gtacttgcgc tcgacgtccg
     2161 tgagcccgtg ccacttgtcg gggagcggcc ggagcgactt cgcgaggaag tcgagcgact
     2221 cccccttgac cgagagctct cccgtgcgcg tccggaagac gacgcccgcg acgcccacgt
     2281 ggtcgccgag gtcgagctgc tcgagaagct cccagtcgcc ggggcgcagg aggtccttct
     2341 tcaggtacga ctggacgcga tgggcgccgt cggagagatc gacgaagatc gccttgccca
     2401 tcttgcggat cgcgaggatg cggccgggaa cccgcacgcg gcggtcggcc tcgggctcgg
     2461 cctcgagggc ggccgcgtcc ctcgacgacc agcgcgcgac gacgtccgag acgaggtgcg
     2521 tggagtccca gcggttcggg tagaggggcc ggccgagcgc cgcgatgcgt tcgcggttcg
     2581 cgcggcggtt cgcgatctgc tcggggacgt ccgcctcggg ggctttcggc tctgcggttt
     2641 ttttcgggtc cggagtctca gacatcgagg cggattaaac cacctcgccg cgcccaggcg
     2701 cgctactcgc cgccgagcga gctgtagagg agccagaggc ccgcgaaggc cctgaggtgc
     2761 cggaaccgcg agcgcgtgat ctgcttccag gccgcgcggt cgttctcggc cgcgagaagc
     2821 gcttcgcgga acgtccggtc gacctcccgc gcgcggacga ggcacttgcc cgccggcgcg
     2881 atcgtggcgt ccgaggccga cgactcgccc acctcctgca cgaggtcgcg gagtttctgt
     2941 gcctcctcgt ccatccggca ccggaggacg gccccgttga aatagaggcg ctcgtgagcc
     3001 gtgagcggct cggaatccgg ctcgtggttc gcgcgataga cccggagcgc cgcgcggacc
     3061 tcccagttct cgaggcgcgc cgaggggtcc gtcacggacg gtcctcctcc cgagcgcacc
     3121 aggtccacgg ccgaaaggag gcgccgcgcc gtttcgctcg tgacccggtc gttctcggcc
     3181 ctccacccgg cgacgctcct cggcacgggc gtcgatccgg aggctgcgga cttgcggcgg
     3241 cgcaccccgc ttccgctccg cccgccggcg gatcggcccg cggtcgagtc ggacccgatg
     3301 tcgagttcgt cggcggccgc ggggtcgagc cgggcgagga ggcgatggag cgattccttc
     3361 aggcgcttga cgtcccgggg ccgcacgccg tgcttgcggc tggccctctc gaagtcctcc
     3421 ttgtccctgc ggaagcgctc gaggtcctcg cggaactcgg cgctcatgaa ccgcgtgtcc
     3481 ttctcgaggt ccttctcctt cgcgatcacg tcgcgcgagg ccgcgaggat gcgccgctcc
     3541 tcctcccggt agaggagctg gaacttcttc ttgaacgcga cgttcgaggc gaggacggcc
     3601 tccatgacct cgggcgagta gaacgcggcg ccgagccggt gcttgagcgt gcggaggttc
     3661 tcgagcgtct tcttcttcgt gaggtcctcg aagcgctcac aggcgtcgag gtcccgtcgc
     3721 aggacgtcca ggagcgtcac gacggcctgg acctccgacg gcgcgatcgg cgggcgggcc
     3781 gtgaggccga ggagcgcgcc cgagagcttc tcgaggtcgg cggcgggctt gaggacgatc
     3841 tcgccatccg cgtccttcgc ggtgccgagg cgcgtgagga ggaagtcgac cttgtccatc
     3901 tcgtccgggc cgaggctcga cgcgtagtag tagaacttca ggatcgagag caggagctcc
     3961 tcgtcgcccg ccttcacgcg ctcgaagaac tgccggagcg tgctcggggg aatgagacgg
     4021 tcgtcgtccg cgagggtggc gtgcaggccg tccagctcgc cgtccagccg ctgcattgcg
     4081 gccttcgagg cgtcctcggg ctcgacgccc ttcgtctcct tgatgcgctt gatctgttcg
     4141 tagagcggcg cgaacgggac gtccggcagg ggcgccccct tcaggctcgt gcggtgcacg
     4201 ccctgcagga actggtggaa catccacagg gccttgaact tctccgagag gcgggcgtac
     4261 gcccgggaaa cgctctcagg ctcgcgttct gcgtcgctca cggtggccat ccattctaca
     4321 ggcgtcgttc cggggcccgg actttcccga tgatatcttc cgcgccgtga tcgaccacat
     4381 cctgctctcc gaagagacca tcgcccggcg cctcgatgtc ctcgcgaagg agatcgacgc
     4441 ccgctacgtc ggcaaggacc tcctcgcggt cggagtcctg aagggctccg tcttcttcct
     4501 gaccgacctt ctgaagcgcc tcaacgtgcc cgtcgcggtc gacttcatgc aggtgcgctc
     4561 ctacgcgggc acgagctcgt cgggcaacat ccagctcctc aaggacctct ccatggacgt
     4621 cgcgggcaag gacgtcctgc tcttcgagga cgtcgtcgac acgggcctca cgctccgcaa
     4681 gatcctcgag ctcctctcct cgcgcaaccc gcgctctctc gggatcgccg ccctcctcaa
     4741 gaagaggctg cccgagaatc gcgacatctc cgtggacttc ctcggcttcg aaatcggccc
     4801 cgagttcgtc gtgggctacg ggctcgacct cgacgagaag taccgaaacc tgccgcacgt
     4861 ggcggtctgg gacccgaaaa aatgagcgac gtcaagacca tcgccgtcga caacgccccg
     4921 aaggccatcg gaccgtacgc gcagggcatc gccgccgggg ggttcctctt caccgccggg
     4981 cagacgccgc tcgacccttc gacgatgaag ctcgtggaag gcgacgtcgc cgcgcagacg
     5041 acccgcgtcc tcgacaacct cgaggccgtg ctccgcggcg cgggcctgac gttcgcggac
     5101 gtcgtccggg cgggcgtctt cctcgtggac atgggcgatt tcgcgaagat gaacgaggtc
     5161 ttcgcggcgc gcttcgggag ccaccgcccg gcgcggacga ccgtgcaggt cgcgggcctc
     5221 cccgcgaacg cacgggtgga gatcgacctc gtcgcgaagc tacactaggg gttcgcgcgg
     5281 cccgacggcc gccgccagga gaaccccatg agccggtctt ccgcgatcct cgtgttctcg
     5341 gccctgctcg cctttcccgc gctcgcggac gacgcgacgc ccgccccggc ggcgacgcct
     5401 gcggctgcgg cgaagaagaa ggccgcgaag aaggcggcgc cgcagcccgc ggcgggctcc
     5461 gcgaacatga tcgtcacgaa ggacgccgag accggtgagc tgcgcccggc cacgccggcc
     5521 gagcgcgaga agctcctcgg gcggcagccg ctcgcggcgc ccgagcacag ggtcgtcacg
     5581 ctgcccgacg ggtcgcagat ggtcgagctg accgaggccg atcgctccta cgccgtcgcg
     5641 acgaagaacc ccgacggcac gatctcgcgc acctgcgtcc acggagccgc ggaggccgcg
     5701 aaggccgtcg cgtcgcccgc tcccgcaacg gtcgccgcgc cggcgcccaa gacggatcgc
     5761 tgaggacggc gccgatgaag acgcacctcc tgcgggccac cctcgccctc gtgctcgggg
     5821 ccgccgcgct tccgctcgcc gcggccgact tccagatcgt cgtggcggac ggcacgggcg
     5881 tcggcttcaa cgacccgacc ccggcgaccc cggtcggcgg gaacaccgga acgacgatcg
     5941 gcgcgcagcg cctcatcgtc ttccaggagg ccgcgcgcat ctggggctct ctccttccgt
     6001 ccaacgtgac gatcaaggtc aactccagct tctcggccct cacgtgcagc gccacgaccg
     6061 cggtcctcgg gtctgcggga gcgacgacgg tccacgcgaa cttcgcgaac gcgcccatcg
     6121 cgaacacctg gtacaacaag gccgaagccg acaagcacaa cggggtcccg ctcggtacga
     6181 accccgcggc catcaacgct caattcaaca gcgagctcgg gaagaccggc tgcctgaccg
     6241 gcacgttctt ctacctcggc ctcgacaaca accacggctc gaacgtgaac ctcctctcgg
     6301 ttctcctcca cgagttcggc cacggcctcg ggttcacttc ggcggcgaac tcgagcggca
     6361 agttcatcgg ctcgggcacg aaccagtatc ccggcgctta cgaccgcttc ctctacgaca
     6421 ccgccgccgg caagacgtgg gaccagatgg cgacggacgc cgagcgcgtg acctcgggga
     6481 cgaacaccgg caggctcgtt tggaacggga cgaacacgac cacgtacgcg aaggcgtaca
     6541 tgagccgcaa ggccaacctc gtcgtgacgg cgcccgccgg ctccgcgggg acgtacgccg
     6601 tcggcaccgc cgacttcggc gcctcccccg ccgtcgtctc cgtctcgggc cccgtcgtga
     6661 tcgcgacgga cgccgccgac ggcgccggac cgctgacgac ggacggttgc agtgcgatca
     6721 cgaacggccc ggctctcgcc ggaaagatcg ccctcgtcga ccgcgggacg tgcgcgttcg
     6781 tcatcaaggc caagaacgtc cagaacgccg gcgcgatcgg gatgatcgtc gccgactggg
     6841 acccggccgc gggcgtcgcc gggatgtccg gaaccgacgc gacggtcacc attccctccg
     6901 tgcgcgtgac cttcgacgac gggaaccggc tgaaggcggc gctcccgacc gcggcgacga
     6961 tcggccccga ccccacggtc ctcgcgggca cggacgccac cggccgcctg ctcatgtacg
     7021 cgccgaaccc gttcgagagc ggctcgtccg tgtcgcattt cgacacgacc gcctccccga
     7081 acctcctcat ggagccgaac atcagcggcg acctcccgat cgccgtcgac gcgacgctcc
     7141 cgctgtttcg cgacatcggc tggttcgagg gctcgacggc gatcccgacg acgtggattc
     7201 ttccttcgag cgcccacgcg cagggcgcga acggcgcctt ctacaagacg gacctcaccg
     7261 tctcgaacac gggggccgcc gcggccaacc tggtcctgaa gttcctcggc cacgacgcgg
     7321 acggccgaac gggcgcggag gtcacgcgcg tcgtcccggc cgggcagacc gccacgtacg
     7381 cggacgtcct ttcctcggtc ttcggtgtct cgagcggctt cggcgcgatc cgggtcaacg
     7441 cggacacgaa caacctcagg atcgtgagcc agacctccac gccgccgccc tccggcgtcg
     7501 gcacgttcgg ccaggccgtt cccgcggcca cgggtaacga tttcgccacg acggcggcgc
     7561 cgaaggccct gttctccctg cggcaggacg cggcgttccg gacgaacgcc gtcatcgcga
     7621 acgcgacgga ggccccggcg cacgtggacc tcgcgctcta caactcgtcc ggcgttccga
     7681 tcgggagcgg cgctgcagac ctcaaccctc tcgagatgcg gcagatcggc gccgtcatca
     7741 cgagcctcgg cgggcccgac ggcacgaagg acgccttcct cgtcgtgtcg acgccgaccg
     7801 ccgacgcgcg gatcgcgacg tacgccgccg tgatcgacca gaacacgaac gacccgcgca
     7861 cgatcctccc cgcgacgctc gggacgctcg ggacgaacgg cgcctgggtg ctcccgtcca
     7921 gcgcgcacgc gcagggcgcg aacaacgcgt tctacacgac ggacctcacg atcgggaacg
     7981 ccgggacgac cccggcgacc gtgacgctca agttcctcgg ccacgaccag gacgggagcg
     8041 gcggccccga ggcggtccgg acgatcccgg cgaactccgt cgcgacgtat cccgacgtcc
     8101 tcgggtccgt cttcggcgtt tcgagcggct tcggcgcgat cctcgtcacg tcgacctcgt
     8161 cgaacctcaa ggtcctgagc cagacgtcga cgccgccgcc gagcctcgtc gggaccttcg
     8221 gccagtcggt ccccgcggcc ggcgccgccg acttcgtgac gctcgccgcg ccgaagacgc
     8281 tcgtcggcct gaggcaggac tcggcgttcc ggacgaacgc cgtcatcgcg aacgcgaccg
     8341 ccctgcccgc gcatgtcgac ctcgtcctcc ggtccgaggg cggcgccgtg atcggctcgg
     8401 gttcggcgga cctgctcccg tacgagatgc ggcagatcgg cgccgtcgtc acggcgctcg
     8461 gcgcgccgga cggcacggcc aacgcggcgc tcgtcgtctc gacgaccacg ggcggcgcgc
     8521 ggatcgcgac ctacgccgcg atcatcgacc agaagacgaa cgacccgagg accgtgctgc
     8581 cgtagccgct tcgcggcccg gggatttcct agaaggtgaa gagaaagagg gtcggcggaa
     8641 acgccggccc tttttcgtca gtggcctttc gcgatctcgc cgaaggcgcg gagggcgcgc
     8701 tcgacgtcga tgtcgttgac gtcggcgtgc gtgaccatcc ggatggacgt ggcgcccgca
     8761 gccccagcca ggactccgcg ctccttgagc ttcgccacga ccgtagccga gtcgccccat
     8821 ggaccttcta agaaaaacaa cagaatgttc gtctcgacct cgacaggatc gatgcgaagc
     8881 ccccgaatcg aggcgagccc cgccgcgagc ttcgtcgccc gcgcgtggtc ctccggcagg
     8941 cggctgcgct cctcctcgag cgcgacgagc gccgcagccg cgatcacgcc cgcctggcgc
     9001 atccctccgc cgaagagctt ccggacgcgc cgcgcctcgg cgatgaagtc cttcgagccc
     9061 gagaccgcgc tcccgaccgg ggccctcagc cccttcgaga agcacgtcat gaccgagtcg
     9121 aagccggccg cgagagacgc ttccgtgacg ccgagagccg cggcggcgtt ccagaggcgg
     9181 gcgccgtcga ggtggacctt caacccgtgc tgcttcgcga gcgcgaggag cggatccgtc
     9241 gcctcgcgcg gcgtcacggt gccgcccgcg ccgttgtgcg tgttctcgag cgccaggacg
     9301 gacgtgcgcg gcaggtagta gacggattcc ggccggatcc acgtggcgac gtcgtccggc
     9361 gcgagacgcc cgcggacgcc ctcgaccggg cgcggaagga ggccggagag cgcggccatc
     9421 cccgccatct cgtagtggaa gatgtggcac ctgctctcca ggatgacctc ggttccggac
     9481 gcgccatgga cgtgcagcga gagctggttt cccatcgaac cggtcgggac gaagagcgcc
     9541 gcctcgtgcc cgaggacctc ggcggtcttc tcttcgagac ggcggacggt cgggtcttct
     9601 ccgtagacgt cgtcgccgac ctcggcctcg gccatggcct ggcgcatgcg cggcgtaggt
     9661 ttcgtgacgg tgtcggagcg gaagtcggag atctgggcgc tcatgcgccg atcatcccag
     9721 aacgagcgcg gcgagggcga ggaggaaacc caggaaatcg atcttgctcc cgcgcgcccg
     9781 agcggcgtcg cggcgggcat gccagggcgc tcgaagattt tcttcgaatg tggaagagcg
     9841 ctcgtcgaag taccgggcct tcggcgccac gaccatgacg gagtaaacca gcgacgcgag
     9901 gagcacgaag atcgtcacgg cccgccacac gtggcgtgag ggcagagagc tgcccagcac
     9961 ctgcacctcg agcagcactc cgaggagcag cgcgcccgca gccacgaacc gcgcccggcc
    10021 caggcgctct ctcccctctt ccccttccaa gaagttcttc tgctctttct ctttctcttc
    10081 ttccccccgc gtcagccagc cgaagatccg ggcgaggggg atgcccacga agacggacag
    10141 gccgagcgcc acgaggacgc gggaagcctc cagcggaagg tcggcgaggg gcatcggtcg
    10201 agtctagcgg gataatgggg ccacacatgg gcctctacct catcgagacg tacccggtcg
    10261 ggccgctcca gtgcaactgc gcgatcctcg ccgacccctc gacgcgtgag gcggtcgtcg
    10321 tggatcccgg cgacgagccg gaccgcatcc tcgagacgct ctccggcgcc ggactgaagg
    10381 ccatcgcgct cgtccacacg cacgcccact tcgaccacgt gggctgctcg gggctcctca
    10441 agcgcatcac gggagcgccc atcttcatgc acgaggacga ccggccgctg taccagaacc
    10501 tcgacgagca gggaaaggcg ttcggaatcc atctggaaaa gcccggcatc atcgacaagc
    10561 tcctcgtcca cggcgaccgg atcccgatcg gttcgggcac gctccacgtg ctccacacac
    10621 ccggccatac cccgggaagc ctgtcgttcc ggatggaggg cgccgaaggg gacattctgt
    10681 tctccggcga cacgctcttc cggcgctcga tcggccggac ggacctctgg ggcggctccc
    10741 aggaccagat cctcgactcg atcaggcgcg agctcctgac gctgccgggc ggcctccgcg
    10801 taatcccggg ccacggcccg gacacgacga tcgccgagga gggccgccgc aatcccttcg
    10861 tgggcgaccc cggctacaag cccgcctgac ctgctttatc attcccgcac atgagactca
    10921 cgtacgaaga gagcgcgggg ctgctccaca agttctggtc gggcgaaccg gtgccgtctc
    10981 cgaagacggg caggccgatg cgggccttct tcgtcgagcg cacgttcgcg ccgcaggtcg
    11041 tcatgatcgg gacgaagggc gagcacttcc gcttcgacca gaagccgcgc cagatcgtct
    11101 tctcggcgtc gcacatcaag tcgatggtcc gcgacgccaa ggagcgcgag aacccggtct
    11161 gcccgcagga cttcgaggcg ctctacgtca agcgcatccc cgcgacgcgc ttccccggcg
    11221 agtgggactg gacgttctgc tgcccgaagt gcctctcgtt cggcacgtgg gagtacacgg
    11281 acaagaagga cgaatccgcg atcccgccga cgaagaagaa ggaagaggct ccgccgttcc
    11341 acgacgagac gatcccgctt ccgaagcgga tggagatggc gaagacgggc gacctcgcgc
    11401 tcaagctcta cgcgtcgatg gcgcagaccg actgcacggc ctgcggctac gactgcgagg
    11461 gctacgccaa ggccatcgcg aacgggaccg agaagaacct ctcgctctgc gtgccgggca
    11521 aggaagagac cgaggacgtc atcaagcggc tcatggcggg gacctacacc ttcgcctgag
    11581 cctccgcgag catcgcctcg atgacggggc ggcagcgctc gcccttgcac tcccccgtcg
    11641 tgcagcccgt cgccgccgcg acgtcctcga ccgtccgcgc gccccgcgcg atcgcctccg
    11701 tcacgcgcgg accccggatc gtgttgcaga tgcacacggg ccggaacttc agcagcatga
    11761 gctgacgctt ctcttcggtc gtcagaggtc gggcggcgga catgcgttcc ccgtccggca
    11821 ttgtacggac cggtgacgac gacgcccctg gagacggccg ggacccttac cggcgcccgg
    11881 tgagccgcgc gagctccgcc tcgccgtctg caacctcccg ggcgagctcg gagtctgcgg
    11941 gatccttgtc ccgccacgac cggaggatgt ccagggctcg ggcgagggtc gcgcgggcgc
    12001 gcgaggcgtc cccgccccgc gcctggaggt tcgagagacg cgcgaggccg cgggcgagcc
    12061 cgaggttcgc ccacttgtcc gcggggtccg cccgcgcgat ctcttcccga aggttcgtcg
    12121 cccgttcacg gaatgcgatc gcgcccggca ggtctcccat ctggcccagg caggcgctca
    12181 cggagccgag gctgaacgaa aggtccagcc gaacgagggc gctgttcgca tcggcgcgca
    12241 ggcgttcctc gtcgatcgcc acggcctgct cgtagagtcg gagggcacct cccaggtctc
    12301 cggcgcgctg gagaagcgac ccgtagtact tgcagctcag ggcaagctcg cgacggaccc
    12361 ggccctcctc cggcttctcc ttcgcgagct cctcgaggat cgcgtgggcc gcccggcgct
    12421 cctcgagggc ccgcgcgaag tccttcgtgc ccgcatgggc cgacgagagc gaggcgtacg
    12481 tggccgactg cgcgagccgc gccgcgaagt cgcccggatg ccgggcgagg atctccttct
    12541 cgatccggag cgcctcggcg aactcctgct cgtacgagtc gtcgacctgt ccaagcgcca
    12601 cgtgcagatt cgcgagctgc agccggtccg cgtcggtcgc gaaccgcgag tccaccaggg
    12661 cctgcagcac gcggatcgcc ttccggaagc tctcccgcgc gcccgcggcg tcgcccaggt
    12721 tcgagttccc gcccccctgc acggcgccga gccgcccgta gctcgccgcg atctcgcgct
    12781 ggaggtccgg attgagggat tcccccgccg cgagcgcgtc cacgtattcc aggcccgtcc
    12841 ggacgagctt ctcgcgggcc ttcgtcgagc ccgggagcgg ggcgatcgtg tcgtgcaggt
    12901 cgaacatcac ggcccgcgca agcttccgga ggtcgtcgaa gcgccgcacc gcgatgcgcc
    12961 gctgctggtt cgcgacgagg aacccgccga gcagcgcggc cgccaccgcg gcggcggccg
    13021 cgagaccggc ccagtggcgc ctcacgaact tgcgggcgcg gtagagcgcg cccccgtggc
    13081 gcgcgagaac gggatagccc gcgaggaacc ggctgacgtc gctcccgaac tgctcgaccg
    13141 agccgtaccg ctcctccggc tccttccgga gggccttgag gacgacgacc tcgacgtcgc
    13201 tcgggaggtc cgggtttcgc gagcgcggcc gggccggctc ctcggcgagg acggcgtgca
    13261 ggagcgcggc cggctcggac gaatcgccgt acggcctttt ccccgtgacg aggacgtaga
    13321 gcaggacgcc gagggagtag acgtcggaaa gcgtggacac gggcccgccg cggacctgtt
    13381 cggggctcgc gtaggccggc gtgagagcgt gccactccgt ggccgtccgt tcgtcccctc
    13441 cctccggggt gaggagccgc gcgaggccga agtccaggag cttcggcact ccgtccggcg
    13501 tcacgaggat gttcgacggc ttcatgtcgc gatgcacgac gagattgcga tgggcgtact
    13561 ggacggcgtc gcacacgagc aggaagaggc gcagccgctc gtcgagcggg ctccccttcc
    13621 gatcgcagta cacgtcgatc ggaaggccgt ccacgtactc catgacgaag tacggaaggc
    13681 ccccgtccgt cgtgcccccg tcgaggagcg tcgcgatgtt cggatgagag agagacgcga
    13741 ggatctggcg ctccgcccgg aacctcgcca ggatgggtcc ggcctcgagg cccgcctgca
    13801 cgagcttgac ggccacgtcc cggtggaacg cctcgtcggc cctgcgcgcg aggtacaccg
    13861 aacccatgcc gccgcgcccg atttcgcgga tgagttcgta gggccccgcc cggcgtccgg
    13921 cggccgctcc cgggccctca tcctggaggc cggagatcgc ctcgcgcacg ccgtccgtga
    13981 aggccgggcc ttcggcttcc cggaggaggg cctcgacttc ctctcgaacc tcgggagcgc
    14041 cgcaggagcg ccgcaggaat tcggctcgtt ccgtctccgg cagctcgacc gcttcgtcga
    14101 agatcttgcg agccttctgg aagagctcgg cgttcacagg gcccgcatct gccgccgaag
    14161 ccacgcctcg gcgtgccggt agtcgcggct cgcggtggcc ggagagcaac caaccactct
    14221 tccgatttcc tcgaccgtaa gcccggcgaa gacgcgcagc tccacgaggt gtgcctgccg
    14281 cgcgtcgagc tccgccagcc tgtagagagc ctggtcgagg gccaggaggt caagagcacg
    14341 cggcgccacc gcggcgtcct cgctctcgag cgggacctgg aagccgccgg cgcgtttttg
    14401 ggcgtgcttc ttgcgggcgt ggtccacgag cacctgccgc atcgcccggg ctgcaacgcc
    14461 gaagaagtgg ccgcgatcct gccacgcaag gtgctggtcc acgagcttga cgtaggcctc
    14521 gtggacgagc gccgtaggct gcagcgtgtg atcggagcgc tccttccgca tctgagacgc
    14581 cgcgaggcgc cgcaactcgc cgtagacgag cggcatgagc cgctccgggg cgtcgcggtc
    14641 cccgttcgac caggcgcgga ggagcccggt gacctctccc ggcgagacca gcgggcgctc
    14701 tccactttcc gtcatcgtag accgggagaa ttctctcagg aatcgcgagt ggaatggcgc
    14761 gcgatcgcgc attcctcgag tgaggccacg ttatgcgtat cagaacgctc gccctcgtgg
    14821 gaatgttgat cctcgagggc cggattcttg ccggcgacga gctgcccatc gccaactggc
    14881 catccgcgcc ctactggagc ccgacggcgg caagagaggc gcgatcggcg ctcgcggcgg
    14941 tccagccgct gccgttcatc gcgctcacgc cgtgccggat cgtcgacacg cgcggcaacg
    15001 cacccctgac cggcggcttc ctcccggcgg ccacggtccg cagctacacg gtcacgaacg
    15061 tctgcggcat cccgcccggc gccaaggccc tgtcgctgaa cgcgacggtc gtccacccca
    15121 ccggtcccgg cttcctgacg ctctatccgg agggagggac gttcccgccg gtctcgacgc
    15181 tgaactacct cggcaacgac gtgatcgtga acgccgccgt cgtgccgctc agcgccacgg
    15241 gcgggatctc gatggccctc ggcgtctcgg gcggcgacgt catcctcgac acgaacggct
    15301 actacgcagc cgtgccgtcc gtgacgagcc tgaacgccct cagcggcgac ctcacgctgg
    15361 ctgcgggcac caacgtcacg ctgacgccgt cggggagcac tctgacgatc tcggcggcgg
    15421 gaggcggcgg cggtgggggg acggtgacgt cggtcgggac ggggtcgggg ctgacggggg
    15481 gcccgatcac gacgacggga acggtctccg tggctcccct cggcatcacg acggggatgc
    15541 tcgcgaacaa cgcggtcacg tcggcaaagc tggcgaacgg cgccgtgggc ctctcgcaga
    15601 tcgacacgtc gcaggtccag gcgcgggtgg cggcgtgccc gccgggcacg acggtccggt
    15661 cggtcaacgc ggacgggacg gtcgtctgcc gggctcccgt ggtcatcgat ccgatgatct
    15721 ccaccgtcga cggcgcgtcg ctcgccggcc agtactcgtc gatcgcaatc gggtcggacg
    15781 gccgcggcct catcagctac cacgacgggc tcaacggcga cctcagggtg gcccactgct
    15841 cggatgcggc ctgcacgagt gccaccacga caactctcga cgccgcggga gttgtcgggc
    15901 agtggacttc gatcacgatc ggctccgacg gcctcggcct catcagctac tacgacgtca
    15961 cgaacgggaa cctcaaggtt gctcactgct cgaacacggc atgcaccagc gccacgactt
    16021 ccgtgctcga cagcgccggt aatgtcggcc taacatcctc gatcgcgatt gggacggacg
    16081 gcctcggcct gatcagctat cacggcgcga ccgacggcga tctcaaggtg gcccattgct
    16141 cgaacgcggc ctgctcggcc gccaccatca ccttgctcga cactccgggc ggtgtcggct
    16201 tgtacacgtc gctcgcgatt ggttccgacg gtctcggcct gatcagctac ttcgacaact
    16261 ccaacgacga tctcaaggtg gctcactgct cgaacacggc ctgcaccaac gccaccatta
    16321 cgaccctcga cagcgcgggt atcgtcggct cgtacacgtc gatcacgatc ggagcggacg
    16381 gcctcgggct gatcagttac ttcgacaaca cgaacaacca ccttaaggta gcgcactgct
    16441 cgaacgtgag ttgcacagcc gcgacgatgt ccaccctcga cagcgcggcc gacgtcggcc
    16501 ggtacacctc gatcacgatc ggcgccgacg gcctcgggct gatcagctac gctgacgcca
    16561 ccaacagccg tctcaaggtc gcccactgtt cgaacacggc ctgcacgagc gccacgactt
    16621 ccatcgtcgc cgggcccacg caacagacct cgatcaaggt cggccccgac ggcctcgggc
    16681 tgatcagcta cttcgacagc gtcgggttcg gcagcctcaa ggtggcccgg atgggaaccg
    16741 gcggcgcgag cgtcaacggc ctcaccggcc cggtcacgat ctccggcggg ggcgggacga
    16801 ccgtgacgtc gagcggaaac acgctgacga tcaccgcgcc gaccgcgcct ccagcgtggg
    16861 gcctgaccgg caacgccgga acgacgcccg gagccaactt ccttggcacg acagacggcc
    16921 agccgctcgt gatccaggcg ggtggcggcg tgggcgtgaa caagagcgcc ccctccgcga
    16981 cactggacgt cgcggggacg atcagcggcg cgagcagcag cgggaccggc gtggtgggct
    17041 cctcgtcgaa cccgaattcc acgggggtgt acgggctggc ctcggcgtcc acgggcttct
    17101 cgatcggcgt caggggcgac acggcctcga ccaccggcat cgcgatctgg gggtattcca
    17161 cgcccacggc ggccaccggc ttcccagtcg gagtggaggg ccggtcggac tccccgatcg
    17221 gcgtcggcgt gaacggcttc gcagccgccg ggagcggcgt gaactacgga attcgtggcg
    17281 cgacgaactc cccgagcggc tgggcggggt acttcctcgg gaacgtcagc gtcatcggca
    17341 ccctgagcaa gtccggggga tccttccgga tcgaccaccc gctcgacccg gagaacaagt
    17401 acctctacca ctcgttcgtc gaatcgcccg acatgatgaa catctacaac gggaacgtga
    17461 cgaccgacga aaagggttac gcgaccgtcg agctgccggc ctggttcgag gcgctgaacc
    17521 gggacttccg gtaccagctg acggtgatcg gacagtttgc ccaggcgatc gtgtcgagcg
    17581 aaatcgagac cggccgcttc gtggttcgaa cggacaagcc cgcggtgaag gtctcctggc
    17641 aggtgaccgg catccgcaag gacgcgtggg cggagaacca ccggatcccc gtcgaggagg
    17701 tcaagcccga aggcgaacgc ggccgcctcc tccacccgaa ggagcacggt cggccggtgg
    17761 aggacggaat cgcgtggcgg acaccgctcg ccgcgctcga aggacggtcg tcgcccgccg
    17821 agaggagcgt tccatgaggc gcgcggccct cgccgtcgcg ctgctgtcgc gcgctcttct
    17881 ggcagagcgg ccggacattg ccgaccagcc cgcaacctgg accgctccgc cgtattgggc
    17941 gccgccggcc acctcgccgg aagacgcgac ggcgcgcgcg gctctcgcag gacgccagcc
    18001 tctcgtcggt gggcccacgg cgctcccctt cattgcgctg accccctgcc ggatcgtcga
    18061 cacgcgcggc aacgcacccc tgaccggcgg cttcctcccg gcggccacgg tccgcagcta
    18121 cacggtcacg aacgtctgcg gcatcccgcc cggcgccaag gccctgtcgc tgaacgcgac
    18181 ggtcgtccac cccaccggtc ccggcttcct gacgctctat ccggagggag ggacgttccc
    18241 gccggtctcg acgctgaact acctcggcaa cgacgtgatc gtgaacgccg ccgtcgtgcc
    18301 gctcagcgcc acgggcggga tctcgatggc cctcggcgtc tcgggcggcg acgtcatcct
    18361 cgacacgaac gggtactacg cggccgtacc ctcggttacc agcctgaacg ccctcagcgg
    18421 cgacctcacg ctcgccgcgg gcacgaacgt gacgctcaca ccctccggca acacgctcac
    18481 gatcgcggcg acggggggag gcggggggcc tccatccggg cccgctggcg gaagcctgag
    18541 cggcacgtat ccgaatccgg ggatcgcaga cgccgcggtc accgacgcca agatcgcggg
    18601 cgtctcctgg ggcaaggtca cgggggctcc gggcttcgtc ctcaaggccg gggacacgat
    18661 gacggcgtcg ctcaatctca atcccggaaa catcaacctc gtcacggagc cctcgacggc
    18721 gacgagcggc agcatcctca agaacggctt gcggttcctt cacgattatg gctcggtgaa
    18781 tacctttgtc ggcctgaacg cgggcaattt cacgatgacc ggcttcgtca acacggtcag
    18841 cggcaacaac gcgctcgcca ggaacaccac cggcagctac aacacggcca gcggcgccta
    18901 cgcgctcacc ttcaacacca ccggcgactc caacacggcc atcggcgcgt gggcgctctc
    18961 ctcgaacacc accggcctcg aaaacacggg catcggctcc aacgtgctcg gggcgaacac
    19021 caccggcagc tccaacacgg ccagcggcgc ctacgcgctc gactcgaaca caaccggcta
    19081 cgacaacacg gccagcggcc gcgacgcgct ttactcgaac accaccggca acgcaaacac
    19141 ggccagcggc cgcggcgcgc tttactcgaa caccaccggc aacgcaaaca cggccaaagg
    19201 ctacggcgcg ctcgctttga ccacgacggg cagccagaac acggcagtcg ggtatgtcag
    19261 cctctacggc aacacgacgg ggttccagaa caccgcgctg ggggactacg ccggctaccg
    19321 gtccggcgcg agctcgggct acagcggcgg agcgatcacg ttcgtcccgc tcacgaccgg
    19381 cagctacaac acgttcctcg gccggggcac gggcgcgaca gcggccggca tctcgaactg
    19441 cacggcggtc ggcatcgacg cctattgcga cgcgaacgac caggtcaggc tcggcaacat
    19501 cttcgtcggc tccatcggag ggaaggtcgg ctggagcgcc ttgtccgacg cgcgcgccaa
    19561 gcgcgacatc cgcgacctgt cggtcggcct cgacttcgtc ctcggcctca ggccggtcga
    19621 gtaccggctg aaggtcggga acggccgcac ggacatgggg ttcctcgcgc aggacgtcga
    19681 agcgctcctc ggccgcggct acaacgtcgt gaccgccggc ggcgatccgg atcgcacgct
    19741 ctcactccgc tacaccgacc tcatcgcgcc cctcgtgaag gcgatccagg agcagcaggc
    19801 gctgctcgac gggcggagcg cacgcatcga cgagctggac cgccggctcg aggcacggga
    19861 cgccctcatc gacgatctcg gccgtcggct cgcggcgctg gaggagaggg cggcgtcccg
    19921 gcaggagtcg cgcggcccgt ccgaccggcg cacgggacct gcgcccgctc ccgctcccta
    19981 gaagtccacc gtgtagcgga tcgacgtccg gtccttgacg cggtcgaggt agacgacgcc
    20041 gtcgaggtga tcgctctcgt ggagcacgac gcgcgcgtgg aagccctcgg ccacgtacgt
    20101 gacgggcttt ccgctcgtgt cgagcgcgcg aacgcggatc gacttcggcc tcgggacctt
    20161 cgccgcaagg aacggcaccg agaggcaggc ctcgaagtcc tcctcctcgg ccgggtcgag
    20221 gacgtcgaac gacgggttca cgaggatcgt cggcgggacc tcgaggacgt ccttgccgcg
    20281 cttttccccg tgcacctcgt agaggacgac gcgcaggccc gtcgagacct gcggcgcggc
    20341 gaggcccgtg ccgtggtact cgaccatggt ctccatcatg tcctcgaaga acggcttcag
    20401 ccgacccttc ttgaggtccg cgggctcgat ctcggaggcc cgacggcgga ggatcggcga
    20461 gccgagcttt gcgactttca ggatggccac ggaggcattg taccggccgc tccgcctaga
    20521 atccggcatc cgcaacgtct tcgtcttcaa ccccgccgcc ggcaagggcc gcgcggcccg
    20581 cgaccgcgac gccatccgcg ccgcgctgga gaaggcgggc gcgcacggga cgttcgtgga
    20641 aaccgagcgc cccggccacg ggatcacgct ggccgaggac gccgcgcggg acggggccga
    20701 ggccgtgatc gcgatcggcg gcgacggcag cgtgaacgaa gtcgcgaacg gcctcctccg
    20761 gatcccggaa gcgacgcgtc cgatcttcgg cgtcgttccg gacgggaccg gcaacgacta
    20821 cgggtacctc ctcggcctcc ggcccggcga tctcgacggt gcggcgcgcg cgatcgccgc
    20881 cggtgcgaca cgcgtcctcg acgccggcga ggtgaacggc cgtttcttcg cgaacggcgt
    20941 cggcctcggg ttcgacggcg ccgtcgcgga gacggccgcg aaggtgcgtt acctgaaggg
    21001 cttccccgcc tacctctggt cggtcttcaa ggtcctgaag tcgtgggaga acttcgagct
    21061 gacgatgacc tgcgacggcc gcacgatctc gggccgcgcg cttctcgcgg ccgtcgcgaa
    21121 cggcccccgc agcggcggcg gctttcttct cgcgcctgac gcgaaggcag acgacgggct
    21181 cttcgacgtc tgtcgtctcg gcaacctcgg aaggctcgag gcgctccgcc acctgccgaa
    21241 ggcgctcgac ggctcgcaca cggggctgcc ctgggtgacg atcctgcgcg cgaaagaggt
    21301 cgttctggcg tcggaccggc cgctcacggc ccacgtcgac gggaacctcc tgaccggtgt
    21361 cgcgcacccc gacccgcttc ggatcaggat ccttccacgt gcgctgcgcg tcttcgcgcc
    21421 cgccggggcg caggcggcca agccggcgag cgcaagcgag ccggcgaggc acgccgagcg
    21481 gaaggtgcgg gaacccgcat gacgggttcc tgcctaagag taggaagtga tggtgcccag
    21541 gggcggagtt gaaccgccga caccgcgatt ttcagtcgcg tgctctacca actgagctac
    21601 ctaggcactc ggcaccacgc ccgggcacgc ctcttgcgga gggaatcgcc ccggacgcct
    21661 gcgcgttata agtggcgcgc ctagaatagt caaccgaagg gaatcgatgc tgctttctgc
    21721 tttcgctctg ctcgcacaga tgactcaggc gacaccttct gccggggggg gctccatgca
    21781 agcccccccc ggaacccccc ctgctaccgg ttccgcctac gccggcgccg ccggcctcga
    21841 cgtgatctgg gcggccaccg tggagcaggc ccaggagagc gcgcggggga tgaaggacgg
    21901 gcggatcctg ttcttcttca ccgaccccga ctgcggcgag tgcagccgca tgctcaagat
    21961 catcgcgccg tccacctcct tctactcgtt cacgcgcgac aaggttccgg tgcccgtgcc
    22021 catctcgacg cccgagggcc tcaagctcgc gaagaagatg cgcgtcgtgg gggtccccac
    22081 gtggatcgtc gcgacgcccg atctcctcga gtgcagccgg caggaggggg tcacgtcgca
    22141 gcagggctgg gtcaacacgt tcgtcgcggg cgagcgctcc tgggccgagt tccggaaggc
    22201 gcaggccgcc gagaacgcca accccgccga ctccgcggcc gtcttcgagg tcgccaggca
    22261 gctgttccag cgcggcgccc gggagcaggc cgagggccgg ttccgccggc tggccgccga
    22321 ccccgccgtt gctccggaca tccgggagca ggccaacgcc tacgtcgcct cgatcgatct
    22381 cgacgcgggc cgctatgacg aagcggcccg catcctcgac gagctggcca agggggcgaa
    22441 ggacccccgg ctccgggagc gcgccgagct ccgccgcgcc gacgtcgaga tcgcgcgcgg
    22501 gcgccgggac ctcgcggcgt accgtctccg ggaattccag aaagcccatc ccgattcgcc
    22561 gctcgccgcc gaggcccggc agctcctcga cgccctgcaa ggcaaggctt ccgccgcgcc
    22621 tctcccggcg gcggctccgg cggcaaagga gaagtgatgt cgccgatccg gtccttcctc
    22681 gcggcgggcg cgatcgccct cgccgccatc ccgggccgcg cgccggcaca gggcattccc
    22741 aagtcgtccg agatcgtcgc gccgaagctc gtcgccgatt ccgaccggct cgtcgccggc
    22801 aaggcgttcc gtctggccgt cgtggccgac atcaaggccg gctggcacgt gaacagccac
    22861 acgccgaagg aagacttcct gatcccgacc gaggtcaagg tccggcctgc ccccggcctc
    22921 aagttctccg cagtcggata cccgaagcag ctcgaacgca agttcgcctt ctccgagcag
    22981 aagctcgccg tgtacgaggg ccggacgctc ttcgtggtgc ccggcgcggt cgacgcggcg
    23041 gcggccccgg gcccgcgcac gatcacggcc gtcctctcgt accagccctg caacgacaac
    23101 cagtgcctcc cgccggctga gctcacgtcg accctgacga tcgaggtcgt gaagtcgggc
    23161 gaggcgaagc ccgcgaacgc ggacgtcttc gcagcgcctt ccgcgggcgg tgcacacggg
    23221 tctgcgtcgg cggacgccgg ggcggacgac cagctgatcg cgggcgccga attttggccg
    23281 ggcgtcggat acccgctcaa aaaggaggcc gaggtccaca ccgtggtggc ctggcggcca
    23341 acattgtagg tcgtgtagcc ccaaaggcgc agggtgt
//
LOCUS       DAOMFY010000032        23156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_701_length_23156_cov_8.562876, whole genome shotgun sequence.
ACCESSION   DAOMFY010000032 DAOMFY010000000
VERSION     DAOMFY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23156
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_701_length_23156_cov_8.562876"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLB01_04640"
     CDS             complement(<1..194)
                     /locus_tag="PLB01_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76619.1"
                     /translation="MRRARAAAPIAALAALLAGGLPFVVSVTAWPEIVTPAWFVSRGI
                     RLYDGILFPHTPLLILLTATG"
     gene            complement(191..568)
                     /locus_tag="PLB01_04645"
     CDS             complement(191..568)
                     /locus_tag="PLB01_04645"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQQ76620.1"
                     /translation="MRWTVRCHATFEAAHHLVDYVGGPEPVHGHSWKIEVALSVDRLG
                     PYDLSVDFVPTEALVKELASRLHNRDLNTVPPFDRRNASAENVALWVADEIGKAALLG
                     DGARLDEVTVWEGPRNSVTYRPG"
     gene            complement(604..2259)
                     /locus_tag="PLB01_04650"
     CDS             complement(604..2259)
                     /locus_tag="PLB01_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ76621.1"
                     /translation="MAKPVILTVDDDPEVLSAIERDLKQHYRNDYRILKAASGGEALE
                     AARQLKQRGTPVALFLADERMPAMSGTEFLREAIKLHPESRRVLLTAYADTQAAISGI
                     NDVGLDHYLLKPWDPPAERLYPVLDDLLSDWTVHVRLPYDGIRVAGAQSSPRSFAVKE
                     FLSSNQIPYQWIDVDQDPSTRELIRSFGAETKLPVVLFPDGTFLAAPTTRELAEKTGM
                     QTKARLPFYDLLIVGGGPAGLAAAVYGASEGLRTALVEQSAPGGQAGTSSRIENYLGF
                     PSGVSGADLARRATTQARRFGAEVIAQEVVGLRREDPYRVARLADGTDLSCYAVVLAT
                     GVAVRKMEAPGSEALLGIGVYYGAAMTEAATYRGRDVCVVGAGNSAGQGALFFSRYAR
                     RVTMLVRADSLAKSMSRYLIDRIEASSNIEVVTGVEVAAVRGDGRLEKVILRRLAEGD
                     ERELDAAAMFIFIGARPQTEFVAGFLEVDEAGFVVTGPDLTPQGRRPRGWTLERDPFL
                     FESSVPGVFAAGDVRAGSSKRVAAAVGEGSGAVGVVHRYLETV"
     gene            complement(2263..3657)
                     /locus_tag="PLB01_04655"
     CDS             complement(2263..3657)
                     /locus_tag="PLB01_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ76622.1"
                     /translation="MESSIQSRLSAHRLLAGAPADQIAWLAERGHMVTLEPGGLLATK
                     GSMVTGLYIILSGHLTIHVDRGAGPVKTMEWRGGDVSGVLPYSRLVGSPGDVKAEEYS
                     EIFVIPRSEIPALIRECYELTAIFVHVMLDRTRIFTSSDLHEEKMSSLGKLAAGLAHE
                     LNNPASAVARSAKGLRLGLSELEAASRALGAAGLSEAQRAAVDRARGLCMLAAGATFG
                     RSPIEQADREDALADWLGRHGADVTAAEALAESAVTLESLDELAGSLDGAALRTALPW
                     LIAGCATQRLVSEIEASATKIHNLVAAVKGFTYMDQATMPKPVDVRQGLADTLAVLNA
                     KARGKSVSVALDAPGDLPRIPGFGGELNQVWANLVDNALDAAKGRVDVSAARQGPSVV
                     VRIVDDGPGLPPEIRERIFDPFFTTKPVGQGTGLGLDIARRIVRKHQGEIEVDSRPGR
                     TEFRVTLPVETGKG"
     gene            complement(3667..6333)
                     /locus_tag="PLB01_04660"
     CDS             complement(3667..6333)
                     /locus_tag="PLB01_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HQQ76623.1"
                     /translation="MRYASVRPRFEPFLLAVERPGRYLGLERNVVRKDLAAMDVTVCL
                     GFPDAYEIGMSHTGTKILYEIVNRRAAWACERTYAPWTDFEAVLRREDVPLFSVESFA
                     PVRDFDVFGLSLQSEMNYTNVPNMLDLAGIPVLAAERGDAHPVVIGGGPCTANPEPLA
                     DFFDVFLIGDAEEALPVFLEAVARTKGLPRRERLLAFSGCPGIYVPSLYDVTYDGDRV
                     VSYLPNSPGVASEVRRVWVEKLSADVYPAKPMVPSVDIVQDRLGLEIMRGCTQGCRFC
                     QAGYWYRPVRELDPADVAKATKTFIDEAGWSEVGLLSLSSADYSQIEPLVACLAPELA
                     KTKVSISLPSLRAEAFSVALADAVSEVRKSGFTFAPETGSDRLRRVINKTFTNADMVK
                     AADVAFGRGWDMIKVYTMIGLPTETDPDLDELVTLVKDILAQGRKHGVRPQVNVSVGC
                     FVPKSFTPFQWFGFDGVENLTRKLAYLKDRFRSVKGVKLTWHEPKEAEIEAMLSLGDR
                     RMGRAVLEVWKRGGRFDAWSEHFSWERWTEALEAAGVPKSRHLRDKDLKETLPWDVVD
                     ASIRKPFLVVEWKKALKEMHTDDCKWGHCYACGVPGNGDDIVLANPLPGEFSFASDED
                     EDVSRTSEVKGLRPRSGAQSAPLTSDVRATDFHLSAAASAAAAYQKSEEFLPSSSLPS
                     AYTEKAKGAAYRMKAEPDLLPLRQRRSPASGVAAPVPESARTYRLSFEKLGDARYLSH
                     RNTMDVLERALRASGAPVRYTEGYNPHLRLSMGPALPLGHESKHELFDVDTLDALAEA
                     HVAAVNDRLPPGLRITSCTELPKGAKSLGRAATEAVYRFTLPNGETREERLSLMGAAA
                     TTPKKFLEREYGVPPEGQHAVRVTRLETVLGA"
     gene            complement(6393..7016)
                     /gene="msrA"
                     /locus_tag="PLB01_04665"
     CDS             complement(6393..7016)
                     /gene="msrA"
                     /locus_tag="PLB01_04665"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQQ76624.1"
                     /translation="MPTPEEALPGRAEKMPVPATHFVNGHPLEPPFPAGTSAALFGLG
                     CFWGAEKKFWQTPGVFTTAVGYAAGFTPNPTYREVCTGRTGHNEVVLVVYDPAAVAYE
                     DLLKVFWENHDPTQGMRQGNDVGTQYRSGIYAFDAAQKAAAEASKDAYQAKLTEAGYG
                     AITTEILDAPPFYYAEDYHQQYLAKNPDGYCGIGGTGVSCPIGLKTA"
     gene            7157..7546
                     /locus_tag="PLB01_04670"
     CDS             7157..7546
                     /locus_tag="PLB01_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76625.1"
                     /translation="MALDEKLRAYLRELGAALNEAVSSSDKVHEILAKVKSEGYGAYL
                     CLDATVALDKRGRRSSAALPSIRRGGLNASKEEREASGMDTAVFQINVKDLGFLRSVG
                     IDPTRPIRARRNPQAAVTIRSTVAKRG"
     gene            7549..8643
                     /gene="rlmN"
                     /locus_tag="PLB01_04675"
     CDS             7549..8643
                     /gene="rlmN"
                     /locus_tag="PLB01_04675"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQQ76626.1"
                     /translation="MPPQDLLGLTALDLARAVRDVAPEPYRAAQIAKWIYERRAASFA
                     EMSNLPKSMREALAKDFTIGLPKVVARTPAPDGTEKFLFELADGARVEAVYIVESKKR
                     PSWTDPDTPEAARVTICLSSQAGCAVDCAFCVTGRMGAGRNLTAGEIVGQYLVIAREK
                     GFGPHEANVVFMGMGEPLLNVGNVRRTLDLLEEEVSPRRTTVSTAGVVPGIEALAART
                     RRPNLAVSLSGADDETRSKLMPINRTYPIAELFAALKRWPLERGRKITFEWVLIAGVN
                     DRPEDASKLAALVRPLPSKVNVIPLNESAEWLPGLKRPSDAAVDAFARAVAAGGVPVT
                     VRWSKGLQADAACGQLKGRETPRRPAPPAS"
     gene            complement(8597..10639)
                     /locus_tag="PLB01_04680"
     CDS             complement(8597..10639)
                     /locus_tag="PLB01_04680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ76627.1"
                     /translation="MRRTAAVLALAFSGFALAQAPPPADLFALRTVADAQIAPDGKSV
                     AYVVGAWDFRENLLDTDVWLVDVATGASRRLTASPKRDERPRFSQDGKTIAFLSERRG
                     DEKDAKETRQVWLIAPNGGEAARLTSHGSPVASFAWSPDGRRIAFTAAVPPAADEKKR
                     REEKDDANVVDRDDVKPAALWIADVASKAVVRVSGGKEHVSSVEWSPDGTRLIGTVAP
                     TPKVPDGFNTDLVVWEAKENGKVTDLVRRPGPDSNPAFSPDGKWVAFFSGDGRVNDWP
                     GHRTEICVVPASGGPVRNLTRVADLEADDFAWTPDSASILFAAPQKMATQLFSVPAAG
                     GAPKALTSGPHVLGSFSASADGKTLAFVLQSTAEPPEVNVLTASDASPAPRRLTTTNP
                     SVAGALRPKVEVVSWKGPDGLPIEGLLHLPPDRPAQKLPFILHIHGGPAGRFALNHSA
                     FSRIYPIDAFVARGIAVLQVNPRGSSGYGEKFKKANFRDWGGKDYLDLMAGVDMLVAR
                     GIADPARMGVMGWSYGGFMTSWILTHTDRFKFASPGAPVTDLWSFYYTADIPEFIESY
                     FGGMPWDDFDVLKAHSPMWFVKNAKTPTLILHGEADARVPPAQGRELYLALKKRGVPV
                     EMVTYAREPHGPAEPKHVKDIRARLLEWTDRYLGTAAPAAPVKTPAAPGGGASHAP"
     gene            complement(10674..11447)
                     /locus_tag="PLB01_04685"
     CDS             complement(10674..11447)
                     /locus_tag="PLB01_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HQQ76628.1"
                     /translation="MDGHPATRATSGATSGGGVSRLSLPGAPGFAAGFTRGARTARAL
                     DPDLAGRALAKDLGAPDAEVIRLTQVHGRGTLTFEEPPRERRNLLLGEGDAAITCQPN
                     VLLAVATADCVPIVLADPVRGWIAAIHAGWRGTALRVLDAALDALDVRGVSPRDLSAA
                     FGPSIARDRYEVGPEVVAALLDAYGAPPEGAVAPGAGGKAFVDVAAFNEATLLARGVD
                     PARIHRPHVCNAAEPDLPSWRRDGPGAGRILTGVVRPPA"
     gene            11509..12114
                     /locus_tag="PLB01_04690"
     CDS             11509..12114
                     /locus_tag="PLB01_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76629.1"
                     /translation="MKNATLRLPRGLRSPAAIQRFLDGLRYNKEEGGETARSPRRVLE
                     TGEAHCFEGALFAAAALRRLGHPPLVVQLRAVRDDDHVLALFRAGSGTGCWGAVAKSN
                     YSGLRFRSPVYRSLRELAMSYFDCYYNLEGEKSLRSFGRPVDLARFDARGWETAGEDL
                     WDVSTHVATRRFTPLVSAAQARRLPPMDRRLFDAGLVGRRV"
     gene            complement(12111..14762)
                     /locus_tag="PLB01_04695"
     CDS             complement(12111..14762)
                     /locus_tag="PLB01_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ76630.1"
                     /translation="MKLFLALAIAFLMGSAAAQAQDSATVLSGTVTTKEDGQPYPGAT
                     VAIPSLELVARADRNGRYEIRIPAGIAKGRTVDVRVTAPGLAEKSTKVTLAPGPVAQD
                     FPMALGVSAQVTVGSRAAGAEVEKAVPVEVFPASAIEKASGSGEVFQILEKLEPSFNF
                     PRTTISDGTDTTRPASIRSLGPDQFLVLLNGKRRHPSALVNVNGTIGRGSAGVDLNAI
                     PAQALDRIEVLKDGAAAQYGSDAIAGVLNLGLRSGPAPLDVTATTGITNHGDGSVLDL
                     NANGGFPVGSGSINVTGEFRRRNATNRAGDDPRAQGSRDPITQPNTRYGDPATTDYLG
                     FLNGVFPLSADGAVNAYLFGGASHRYADSAGNFRRALQAQNWRTIYPNGFLPKITPTT
                     IDYSATAGVRGVLANHWFWDASVQYGRNQFDFKVVDSLNTSLGPTIPPNQTSFDAGSL
                     VFGQFLANLDVTRQFAIGLAGPLNLALGAEFRNERYRQIAGEPASYFDGGVKAQDGSP
                     APPGAQVFPGFRPANEIDTNRSNVSLYADAEVDVTKQLRVGAAVRFEHYTDFGNSTNY
                     KGTLRYEVLPWLVARGSASTGFRAPSLHQSYFSTVSTNFLNINGVNQPFDILTARVDS
                     GVAQALGATPLKPEDSVNFAGGLVLSPSRDFDFTADYFNIAIKDRIIFSGNFTGAALN
                     PILQPFGASGVRFFTNAIDTRTDGVDLTANYRADLGPNGAVRLLAAWTHAETSVTNIA
                     STPPQLAAFQTTLFDRLEVRRLECGQPKDVVRGTVDYTKGPLNVVVNATRYGSFCGIA
                     NGSGEAPFGPGTAADQTFSAQWLLDLELSWVFSHLRVAIGAQNLGDATPDSTLFVNSN
                     SGINRYPNNSPYGYNGTFAYTRVSYRF"
     gene            complement(14841..16265)
                     /locus_tag="PLB01_04700"
     CDS             complement(14841..16265)
                     /locus_tag="PLB01_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQQ76631.1"
                     /translation="MSSPVAVPLWAFVLLAFLASWAVLDRMLRPAARAVMRRRANRVI
                     DELNARLPVRLPPFKMTKREVLVDQLRFDPRVLEAAEEYARETGQPRELVQKRIERDA
                     REIVPAFNAWIYFRAGYAIARAVARSLYRVRLGTADDAGLRAIPEGSTVVFVMNHRSN
                     MDYFLLSYLAAEKTALSYAVGEWARIWPIQGFLRATGAYFVRRDSKDPLYRRVLERWV
                     AMATANGVPQAVYPEGGLSKDGALRPPKLGLLDYMLRGFDPKGPRDLVFVPVGVNYDR
                     VLEDRTLLLDRSPAQGKASASDTLSETLRFVRRNARLVIANEWHRFGYACVSFGSPVS
                     TRAWLAARNLDLGAASREARFAAVEDLAKDLMERIARVIPVLPVSLVASVLVDAPERA
                     WSELELKAEVLARWRSLEAGGAYVHVPRGDQDYAVSVGLRALLLRHVVVTDGGLHRPV
                     PEELPLLRYYANAIAGAGGAPTAP"
     gene            16321..17412
                     /locus_tag="PLB01_04705"
     CDS             16321..17412
                     /locus_tag="PLB01_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ76632.1"
                     /translation="MERRICLFGARALLLGLCAAAPAARAQVVPVEPVPNEAILRDFR
                     FSSGETLAELKINYVTYGRRLDDGRGKTANAVLVLHGTGGSSAQFLGPTFAGVLFGPG
                     QPLDASRYYIVIPDGIGHGGSSKPSDGLRAKFPRYTYDDMVRAQHRLLTESLGVTHLR
                     LVIGTSMGGMHAWVWGETHPDFMDALLPLASLPAPIAGRNRMWRKMAMDLIRGDPAWK
                     GGDYTEQPPGLRGALSLLLTLSGSPLGWMQQAPDRAAADAAVGKYMSERLAKTDANDL
                     LFALDASRDYDPWLGLEKIRAPLLAINFADDAINPPELGILEKGIARVKNGQAVVFPL
                     SEKTRGHQTHTLADVWKTYLNELLVRSRP"
     gene            complement(17420..18613)
                     /locus_tag="PLB01_04710"
     CDS             complement(17420..18613)
                     /locus_tag="PLB01_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQQ76633.1"
                     /translation="MPPVHEENRVTRALAARTRPHLDLTVSNPTAVALRSGGVDLFLD
                     APGDAGLLAPLADPRGLVYEPDPRGLRRARLAVSNWYAAVHGVLAPPEHLVLTASTSE
                     AYGWLFKVLADPGDAVLVPAPSYPLLDALAGLESVALARYPLAAEDGFRVHASAVARE
                     VARLAAEGTRTAAVVVVNPNNPTGSSIGAAELDALLTLAAERGFAVISDEVFVDYRYS
                     DRPDDVPVAAVRSDALVFSLGGLSKSAALPQLKLGWILANGAEPVLGDALRRLEWVAD
                     TYLSVGTPVQLALPRLLETSRRAAEAIWARVLKNERALRTAFPAGGAVTVAPVAAGWA
                     ACLRVPAVRSEEEIVLDLLETHDVLVHPGYFYEFPSEAWLVVSLLPPPDVFAEGAARL
                     ARALH"
     gene            complement(18640..20745)
                     /locus_tag="PLB01_04715"
     CDS             complement(18640..20745)
                     /locus_tag="PLB01_04715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ76634.1"
                     /translation="MLKRSAPLALALLLLAASFAPAADTGRAFTLEDLYRTKGVGEPA
                     ISPDGRTIVYSVTTNDWAAKKRTVALWRVDADGSNARPITAGDARDEHPFFSPDGKTL
                     AFVSTRTGEPQVFFLPLAGGEAEQKTRFPGGVAGPAFSRDGRFLVFAADVLPACGADA
                     ACNKKASDARDANKIKAHLADSLLYRHWTSWKDGTRTHVFLLDLAELDAGKAIRDLTP
                     GDFDSPGFSLGGGGDFDLSPDGKELAFSSNRDADAAFSTNADVWTVPAGGDAAALSAP
                     KNLTAANKGFDGSPRYSPDGRFLAFKRQLTPRYEADRFRIIVRDRATGAERDLTATFD
                     DNVRDFEFSKDGTRIFFTAEVKGRTPLYEIVLATGALRAVSSVGSLDAWALAPDASFA
                     VAARRRIGSPLELWRLGVATGGEGTRLTTHNAAFEKEVDIRPAEEITVPGAGGKPVHV
                     WIVKPHRFDPAKKYPLILNVHGGPQQQWADAYRGDWQVYPGAGYVVAFPNPHGSTGFG
                     QAYTSAISGDWTGKVMTDIEKVTDALAKLPYVDADRMGAMGWSWGGYAMMWLEGRTTR
                     FKALASMMGVYDLRAMYSSTEEVWFPHWDLKGAPWENPALYKAQSPSEFVTAFKTPCL
                     VITGEKDYRVPYTQSLEFFTDLQARGVPSRLIVFEKAGHWPSAFEMALYYAAHLDWFS
                     RWLGGPPSPWKPEDLVAGR"
     gene            complement(20782..21270)
                     /locus_tag="PLB01_04720"
     CDS             complement(20782..21270)
                     /locus_tag="PLB01_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HQQ76635.1"
                     /translation="MAKKNKKKGHGADAVVATLQRQQANALVTYLNYKKYHWLSYGPL
                     FRDIHLLLDEQAAQTYASIDELAERAIMIGGAPIGDPADYLPAATVKPSEGVLGMREM
                     LTEARAALDVVIKEMHADAEVATDAGDIGTADLYTRLVQVHQKNRWFLDEILKKRDGL
                     TS"
     gene            21364..23040
                     /gene="ettA"
                     /locus_tag="PLB01_04725"
     CDS             21364..23040
                     /gene="ettA"
                     /locus_tag="PLB01_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQQ76636.1"
                     /translation="MAEQYIFTMQNLRKVVPPQREILRGIYLSFFPGAKIGILGLNGA
                     GKSTVLRIMAGVDKEFTGEAYPAEGTRIGYLPQDPQLDPKKTVLENVEEGVAPLKALL
                     KRFDELGEKMGESLPDAEMDKVMAEYGKVQDAIEAANGWELDRTLETAMDALRCPPPD
                     ADVTKISGGEKRRVALCRLLLQKNDMLLLDEPTNHLDAESVAWLEQFLKEFPGTVVAV
                     THDRYFLDNVAGWILELDRGAGIPWKGNYSSWLEQKEARLEIEEKTESARQRSLKREL
                     EWVRMAPRARQAKSKARLQAYEQLVSESGIEARGETNEIYIPPGPRLGDVVVRAEGVK
                     KAYGDKLLFDDLSFDLPKGGIVGVIGPNGAGKTTLFKMIMGLEKPDGGKLQVGETVVP
                     SYVDQNREKPDAKKTVHDELSEGNDLVMLGKREMPSRAFCAMYNFKGADQQKLVANLS
                     GGEMNRLHLARTLKRGGNLLLLDEPTNDLDVDTLRALEEALLSFGGCAVVISHDRWFL
                     DRVATHMLAFEGDSKTVWYEGNYQDYEADRHKRLGAEADRPHRIKYRKIG"
BASE COUNT         3519 a         7841 c         8376 g         3420 t
ORIGIN      
        1 cccgttgccg tcaggaggat caggagaggc gtgtgcggaa agaggatccc gtcgtagagc
       61 ctgatgccgc gggacacgaa ccatgcgggc gtgacgatct ccggccaggc cgtgaccgag
      121 acgacgaacg ggagccctcc ggcgagaagg gccgcgagcg cggcgatcgg ggcggccgcg
      181 cgcgcccgtc tcacccgggc cggtaggtca cgctgttgcg cgggccttcc cacaccgtga
      241 cctcgtcgag ccgcgcgccg tccccgagga gcgcggcctt cccgatctcg tccgcgaccc
      301 agagcgcgac gttctcggcg ctcgcattcc ggcggtcgaa cggcggcacc gtgttgaggt
      361 cgcggttgtg gaggcgcgag gcgagttcct tcacgagcgc ttccgtgggc acgaagtcca
      421 ccgagaggtc gtacgggccg aggcggtcga ccgagagcgc gacctcgatt ttccaggagt
      481 ggccgtgcac gggctcgggc ccgccgacgt agtccacgag gtggtgcgcg gcctcgaagg
      541 tcgcgtggca gcggacggtc cagcgcatgg ccgtcagtct acgacgcgtc gaagcggacg
      601 cgttcagacg gtttcgaggt agcggtgcac gacgccgacg gcgccggagc cctcgccgac
      661 ggctgcggcg acccgcttgc tggagcccgc gcgcacgtcg ccggcggcga acactcccgg
      721 gacgctcgac tcgaacaaga acggatcccg ctcgagcgtc cagccgcggg gccgccggcc
      781 ctggggcgtc aggtcgggcc cggtcacgac gaagcccgcc tcgtcgacct cgaggaatcc
      841 ggcgacgaac tcggtctgtg gccgtgcgcc gatgaagatg aacattgccg ccgcgtcgag
      901 ctcgcgctcg tccccctcgg cgaggcggcg aaggatcacc ttctcgagcc gtccgtctcc
      961 ccggaccgcg gcgacttcga cgcccgtcac gacctcgatg ttcgacgacg cctcgatccg
     1021 gtcgatcagg taccgggaca tcgatttggc gagggagtcc gcccgcacga gcatcgtgac
     1081 gcgccgcgca tagcgggaga agaagagggc tccctggccc gccgagttcc cggcgccgac
     1141 gacgcacacg tcccggccgc gatacgtcgc ggcctcggtc atcgccgcgc cgtagtagac
     1201 gccgatcccg aggagggcct cggagccggg cgcttccatc ttccgcacgg cgacgcccgt
     1261 ggcgaggacg acggcatagc agctcaagtc ggtcccgtcg gcgagccggg cgacgcggta
     1321 tgggtcctcg cggcgcaggc cgacgacttc ctgtgcgatc acctcggcgc cgaagcgccg
     1381 cgcctgcgtc gtggcgcggc gggcgaggtc ggccccggaa acgcccgacg ggaaaccgag
     1441 gtagttctcg atccgcgagc tggtgccggc ctggcctccc ggcgcgctct gctcgacgag
     1501 ggccgtgcgc aggccttccg acgccccgta gacggccgcc gcaaggcccg caggaccgcc
     1561 gccgacgatc agcaggtcgt agaagggcag gcgcgccttc gtctgcatcc cggtcttttc
     1621 cgcgagctcg cgcgtcgtcg gcgcggccag gaacgtgccg tccggaaaga ggacgacggg
     1681 aagcttggtc tcggcgccga acgagcggat cagctcccgg gtcgaaggat cctggtcgac
     1741 gtcgatccac tggtacggga tctggttgct cgagaggaat tccttcaccg cgaagctgcg
     1801 gggcgacgac tgtgcgcccg cgacccggat cccgtcgtag ggaaggcgca cgtgcaccgt
     1861 ccagtcggag agcaggtcgt cgaggaccgg gtagagccgc tcggcgggag gatcccaggg
     1921 cttcagcagg tagtggtcca gcccgacgtc gttgatgccg gagatcgcag cctgcgtgtc
     1981 ggcgtatgcc gtgaggagga cgcgccgcga ctcgggatga agcttgatcg cctcgcgcag
     2041 gaactccgtg cccgacatgg cgggcatccg ctcgtcggcg aggaagagcg cgacgggggt
     2101 cccgcgctgt ttgagctggc gagcggcctc gagcgcctcg cccccggacg cggccttcag
     2161 gatgcggtag tcgttcctgt agtgctgctt gaggtcgcgc tcgatggcgc tgagcacctc
     2221 ggggtcgtcg tcgaccgtca gaatcaccgg tttggccatc cctcagccct ttcctgtttc
     2281 gaccgggagc gtcacgcgga actcggtgcg cccggggcgt gagtcgacct cgatctctcc
     2341 ctggtgcttg cggacgatcc gccgcgcgat gtcgaggccg aggccggtgc cctggccgac
     2401 cggcttcgtc gtgaagaacg ggtcgaagat ccgctcccgg atctccggag gcaacccggg
     2461 cccgtcgtcg acgatgcgga cgaccacgga cggcccctgg cgcgcggcgg agacgtcgac
     2521 ccggcccttg gccgcgtcca gcgcgttgtc gacgaggttc gcccacacct ggttgagctc
     2581 gcccccgaat ccggggatgc gcgggagatc gcccggggcg tcgagggcca cgctcacgga
     2641 tttcccgcgc gccttcgcgt tcagcacggc gagcgtgtcg gccagtccct ggcggacgtc
     2701 caccggcttg ggcatggtgg cctggtccat gtacgtgaag cccttcaccg cggcgacgag
     2761 gttgtggatc ttggtggcgg acgcctcgat ctcggacacc agccgttgcg tcgcgcagcc
     2821 ggcgatgagc cacgggaggg ccgtgcggag ggcggcgccg tcgagagagc ccgcgagctc
     2881 gtcgagggat tcgagcgtga ccgcggactc cgcgagcgcc tcggctgcgg tgacgtcggc
     2941 gccgtggcgg ccgagccagt cggcgagggc gtcctcgcgg tccgcctgct cgatggggga
     3001 gcgtccgaac gtcgcgccgg cggcgagcat gcagagtccg cgtgcgcggt ccacggcggc
     3061 ccgctgggcc tcggacagtc ccgcggcgcc cagcgcgcgg gacgccgctt cgagctccga
     3121 caacccgagc cgaagcccct tggcgctccg ggcgaccgcc gatgcggggt tgttcagctc
     3181 gtgcgcgagc cccgccgcca gcttgccgag cgaggacatt ttctcttcgt gcaggtcgct
     3241 cgacgtgaag atgcgcgtgc ggtcgagcat gacgtgcacg aagatggcgg tcagctcgta
     3301 gcactcgcgg atcagagcgg ggatctcgct gcgggggatg acgaagatct cgctgtactc
     3361 ctcggccttc acgtcccccg gcgagccgac gaggcgcgag tacggcagga cgccgctcac
     3421 gtcgccgccg cgccattcca tggtcttcac cggtccggct ccgcggtcga cgtgaatcgt
     3481 gaggtgcccc gacaggatga tgtagaggcc cgtcaccatg ctgcccttcg tcgcgaggag
     3541 gcccccgggc tccagcgtca ccatgtggcc ccgttccgcg agccaggcga tctggtccgc
     3601 gggtgcgccc gcgaggagcc ggtgcgccga gaggcgactc tggatcgagc tctccatggc
     3661 ggaagtctat gcgccgagga cggtttcgag gcgtgtcacg cgcacggcgt gctggccctc
     3721 gggcgggacg ccgtattccc tctcgaggaa cttcttcggc gtcgtggcgg ccgctcccat
     3781 gagcgagagg cgctcctcgc gcgtctcgcc gttcggaagc gtgaagcggt acacggcctc
     3841 cgtcgccgcg cggccgaggg acttcgcgcc cttcgggagc tccgtgcacg acgtgatgcg
     3901 aaggcccggc gggaggcggt cgttcacggc ggcgacgtgc gcctctgcga gcgcgtcgag
     3961 cgtgtccacg tcgaagagct cgtgcttcga ctcgtgtccg agcggcagcg ccgggcccat
     4021 cgagaggcgc aggtgcgggt tgtagccctc ggtgtagcgc acgggcgccc cggaggcgcg
     4081 cagcgcgcgc tcgaggacgt ccatcgtgtt gcggtgggag agataccgcg cgtcgccgag
     4141 cttctcgaac gagaggcggt aggtccgcgc cgactccggc acgggcgccg cgacgccgct
     4201 cgcgggcgag cggcgctgac gcagcggcag gaggtccggc tcggccttca tgcggtacgc
     4261 cgcgcccttg gctttttcgg tataggcaga gggaagagag gaagagggaa gaaattcttc
     4321 ggatttctga tatgcggcgg ctgctgatgc agccgccgat agatgaaaat ctgttgcgcg
     4381 aacgtccgac gtcaagggcg cgctctgcgc gccgctacgc ggtcgaagac ccttgacttc
     4441 ggacgttcga gaaacatctt cgtcttcgtc cgaagcaaaa ctgaattctc ccggcagcgg
     4501 attggcaaga acgatgtcgt ccccgttgcc ggggacgccg caggcgtagc agtggcccca
     4561 cttgcagtcg tccgtgtgca tttccttcag cgccttcttc cactcgacga cgaggaaggg
     4621 cttgcggatg gacgcgtcca cgacgtccca ggggagcgtc tccttgaggt ccttgtcgcg
     4681 gaggtgccgg ctcttcggga cgcccgcggc ctcgagcgct tccgtccagc gttcccacga
     4741 gaagtgctcg ctccacgcgt cgaagcgccc gccgcgcttc cacacctcga ggacggcccg
     4801 gcccatgcgg cggtcgccga gcgagagcat cgcctcgatc tcggcctcct tcggctcgtg
     4861 ccacgtcagc ttcacgccct tcacggagcg gaaacggtcc ttcaggtacg cgagcttccg
     4921 cgtgaggttc tcgacgccgt cgaagccgaa ccactggaac ggcgtgaagc ttttcgggac
     4981 gaagcagccg accgagacgt tcacctgcgg ccgcaccccg tgcttgcgac cctgggcgag
     5041 gatgtccttg acgagcgtca cgagctcgtc gaggtccggg tcggtctccg tcgggaggcc
     5101 gatcatcgtg tagaccttga tcatgtccca gccgcgcccg aaggcgacgt cggcggcctt
     5161 caccatgtcc gcgttcgtga acgtcttgtt gatcacgcgg cggaggcggt ccgagcccgt
     5221 ctccggcgcg aacgtgaagc cggacttccg cacctcggaa acggcgtccg cgagcgcgac
     5281 ggagaacgcc tccgcgcgga gagacggcag cgagatcgac accttcgtct tcgcgagctc
     5341 gggcgcgagg cacgcgacga gcggctcgat ctgcgagtag tccgccgagg agagcgagag
     5401 gagcccgact tcgctccagc cggcctcgtc gatgaacgtc ttcgtcgcct tcgcgacgtc
     5461 cgcggggtcc agctcgcgca cgggccggta ccagtacccc gcctggcaga accggcagcc
     5521 ctgcgtgcag ccgcgcatga tctcgaggcc gaggcggtcc tggacgatgt ccacggacgg
     5581 gaccatgggc tttgcggggt agacgtcggc cgagagcttc tcgacccaga cgcgcctgac
     5641 ctcggaagcg acgcccggcg agttcgggag gtacgacacg acgcggtcgc cgtcataggt
     5701 cacgtcgtag agcgacggga cgtagatccc ggggcagccc gagaacgcga gaaggcgctc
     5761 gcgccggggg aggcccttcg tccgcgcgac ggcctcgagg aacacgggca gcgcctcctc
     5821 ggcgtcgccg atcaggaaga cgtcgaagaa gtccgcgagc ggctcggggt tcgcggtgca
     5881 gggcccgccg ccgatgacga ccggatgcgc gtcgccgcgc tccgccgcga gaacggggat
     5941 ccccgcgagg tcgagcatgt tcgggacgtt cgtgtagttc atctcgctct ggagcgagag
     6001 gccgaagacg tcgaagtcgc ggacgggcgc gaaagactcg accgagaaga gcgggacgtc
     6061 ctcgcggcgg aggacggcct cgaagtccgt ccacggcgcg tacgtccgct cgcacgccca
     6121 cgcggcgcgg cggttcacga tctcgtagag gatcttcgtg cccgtgtggc tcatcccgat
     6181 ctcgtacgcg tccgggaagc cgaggcagac cgtgacgtcc atcgccgcga ggtccttgcg
     6241 cacgacgttc cgctccagac cgaggtagcg gccagggcgc tcgacggcga gaaggaacgg
     6301 ctcgaagcgg gggcggacgg atgcgtaacg cacggggctc tccgaacggg gcagtctagc
     6361 ggatcacctg agacggtgtc gcgcggccgg cgtcaggccg tcttcaggcc gatcgggcag
     6421 gacacgccgg tccctccgat cccgcagtag ccgtcggggt tcttcgcgag gtactgctgg
     6481 tggtaatcct cggcgtagta gaagggcggc gcgtcgagga tctcggtcgt gatcgcaccg
     6541 tagcccgcct cggtgagctt cgcctgatac gcgtccttcg acgcctcggc ggcggctttc
     6601 tgcgccgcgt cgaacgcgta gatgcccgag cggtactgcg tgccgacgtc gttgccctgg
     6661 cgcatgccct gcgtcgggtc gtggttttcc cagaagactt tcagaaggtc ctcgtaggcc
     6721 accgccgccg ggtcgtagac gacgaggacg acctcgttgt gccccgtccg tcccgtgcag
     6781 acctcgcggt acgtcggatt cggcgtgaag cccgcggcgt aaccgactgc ggtcgtgaag
     6841 acgcccggcg tctgccagaa cttcttctcc gctccccaga aacagccgag tccgaagagc
     6901 gcggcgctcg tccccgcggg aaacggcggc tcgagcggat gaccgttgac gaagtgcgtc
     6961 gccggaacgg gcatcttctc cgcgcgtccg gggagcgctt cttcgggcgt cggcatgcgg
     7021 agcgtctttc cggcgaagag caaggccgat gattctgctc ctcggacggg cgaaggggaa
     7081 gatccccctc cggcaaaacc gaacgccccg tttccccgat tgactcgccc cggtgctcgg
     7141 cctagattgc cgaccgatgg ccctcgacga gaaactccgc gcgtacctcc gcgagctcgg
     7201 cgccgcgctg aacgaggccg tgtcctcgtc cgacaaggtg cacgagatcc tcgcgaaggt
     7261 gaagagcgaa ggctacggcg cgtatctctg tctcgacgcg accgtcgccc tcgacaagcg
     7321 cgggcgccgc tcgtcggccg cgctgccgtc gatccgccgt ggcggcctca acgcgtcgaa
     7381 ggaagagcgg gaagcgagcg gcatggacac ggcggtcttc cagatcaacg tgaaggacct
     7441 cggtttcctg cgctccgtcg ggatcgaccc gacccgcccg atccgcgccc gccggaatcc
     7501 gcaggccgcc gtcacgatcc ggtcgacggt cgcgaaacgc ggctgacgtt gcccccgcag
     7561 gatctcctcg gactcaccgc actcgacctc gcgcgcgccg tgcgcgacgt ggcgccggag
     7621 ccgtaccgcg ccgcacagat cgcgaagtgg atctatgagc gccgcgcggc gagcttcgcg
     7681 gagatgtcca acctgccgaa gtcgatgcgc gaggcccttg caaaggactt cacgatcggc
     7741 ctcccgaagg tcgtcgcgcg gacccccgcg ccggacggca ccgagaagtt cctcttcgag
     7801 ctcgcggacg gcgcgcgggt ggaggcggtc tacatcgtcg aatcgaagaa gaggccctcc
     7861 tggacggacc ccgacacgcc ggaagcggcg cgcgtcacga tctgtctctc gtcccaggcg
     7921 ggctgcgccg tggactgcgc gttctgcgtc acgggccgca tgggcgccgg ccggaacctc
     7981 acggccgggg agatcgtcgg gcagtacctc gtgatcgcgc gggagaaggg cttcgggccg
     8041 cacgaggcga acgtcgtctt catggggatg ggggagccgc tcctgaacgt cggaaacgta
     8101 cggcgcacgc tcgacctgct cgaggaggaa gtctcgccgc ggcggacgac cgtctcgact
     8161 gcgggcgtcg tccccggcat cgaggcgctc gccgcacgca cacgccggcc gaacctcgcg
     8221 gtctcgctct cgggggcgga cgacgagacg cgctcgaagc tcatgccgat caaccggacg
     8281 tacccgatcg cggagctctt cgcggcgctc aagcggtggc cgctcgagcg ggggcggaag
     8341 atcacgttcg aatgggtcct catcgcgggc gtgaacgacc gtcccgaaga cgcctcgaag
     8401 ctcgccgcgc tcgtgaggcc tctcccgtcg aaggtgaacg tcatcccgct caacgagtcg
     8461 gcggagtggc tgccgggtct gaagaggccg tccgacgcgg ccgtggacgc gttcgcgcgc
     8521 gccgtcgccg cgggcggagt gcccgtgacg gtgcgctggt cgaagggcct gcaggcggac
     8581 gcagcctgcg gtcagctcaa ggggcgtgag acgccccgcc gcccggcgcc gccggcgtct
     8641 tgaccggcgc cgcgggcgcg gcggttccga ggtagcggtc cgtccactcg aggagccgcg
     8701 cacggatgtc cttcacgtgc ttgggctcgg ccgggccgtg cggctcgcgc gcgtacgtca
     8761 ccatctccac gggcacaccg cgctttttca gcgcgaggta gagctcgcgc ccctgcgcgg
     8821 gcggcacgcg cgcgtccgcc tcgccgtgga ggatgagagt cggcgtcttc gcgttcttca
     8881 cgaaccacat cgggctgtgg gccttcagga cgtcgaagtc gtcccacggc atcccgccga
     8941 agtagctctc gatgaactcc gggatgtcgg ccgtgtagta gaagctccag aggtccgtga
     9001 cgggcgcgcc gggcgaggcg aacttgaacc ggtccgtgtg cgtgaggatc cacgacgtca
     9061 tgaagccgcc gtagctccag cccatgacgc ccatccgcgc agggtccgcg atcccacggg
     9121 cgacgagcat gtcgacgccc gccatgaggt cgaggtagtc ctttccgccc cagtcgcgga
     9181 agttcgcctt cttgaacttc tcgccatagc cgctgctacc gcgcggattg acctgcagga
     9241 ccgcgatgcc gcgggcgacg aacgcgtcga tcgggtagat ccgcgagaac gcggagtggt
     9301 tgagcgcgaa gcggcccgcc gggccgccgt ggatgtggag gatgaacgga agcttctggg
     9361 ccgggcggtc gggcggcagg tggaggaggc cctcgatcgg gaggccgtcc ggtcctttcc
     9421 acgagacgac ctcgaccttg ggccggagcg cgcccgcgac ggacggattc gtcgtcgtga
     9481 ggcgcctcgg cgccgggctc gcgtccgacg ccgtgagaac gttcacctcg ggcggctcgg
     9541 ccgtgctctg gaggacgaac gcgagcgtct tcccgtccgc ggacgccgag aacgagccga
     9601 gaacgtgggg gcccgaggtc aacgccttcg gggcgccgcc cgcggcgggc acggagaaga
     9661 gctgcgtcgc catcttctgc ggcgccgcga agagaatcga cgcggaatcc ggcgtccacg
     9721 caaaatcgtc cgcctcgagg tcggcgacgc gggtgaggtt gcgcacgggt ccgccggagg
     9781 cggggacgac gcagatctcg gtgcggtggc ccggccagtc gttcacgcgc ccgtcgccgc
     9841 tgaagaacgc cacccacttc ccgtcgggcg agaacgcggg attcgagtcg ggtcccggcc
     9901 gcctcacgag atccgtgacc ttgccgttct ccttcgcctc ccagacgacg aggtccgtgt
     9961 tgaacccgtc cggcaccttc ggcgtcggcg cgaccgttcc gatgagccgc gtcccgtccg
    10021 gcgaccactc gacggacgac acgtgctcct tcccgcccga aacgcggacc acggccttcg
    10081 acgcgacgtc ggcgatccag agcgccgccg gtttcacgtc gtcgcggtcc acgacgttcg
    10141 cgtcgtcctt ctcctcgcgc ctcttcttct cgtccgcggc gggcgggacg gcggccgtga
    10201 acgcgatccg cctcccgtcg gggctccacg cgaacgaggc gaccggagag ccgtggctcg
    10261 tcaggcgcgc cgcttcgccg ccgttcgggg cgatgagcca gacctggcgc gtttccttcg
    10321 cgtccttctc gtcgccgcgc cgctccgaga ggaacgcgat cgtcttcccg tcttgggaga
    10381 agcggggccg ctcgtcgcgc ttcggcgagg cggtcagcct gcgcgaggcg cccgtcgcga
    10441 cgtccacgag ccagacgtcc gtgtcgagga ggttctccct gaagtcccac gcgccgacga
    10501 cgtacgcgac ggacttcccg tccggcgcga tctgcgcgtc cgcgaccgtt ctcagggcga
    10561 acaggtccgc ggggggcgga gcctgggcga gcgcgaaccc ggagaacgcg agggcgagga
    10621 cggcggcggt acggcgcatg gatcctcctg gcggcaaagt gtgccgaagg gtatcaggcg
    10681 ggcgggcgca cgacacccgt gaggatccgc cccgcccccg gcccgtcccg ccgccacgac
    10741 ggcaggtccg gctcggccgc attgcagacg tgagggcggt gaatgcgcgc cgggtcgacg
    10801 ccgcgggcga ggagcgtggc ctcgttgaac gccgcgacgt cgacgaaggc cttgccgccc
    10861 gcgccgggcg cgaccgcgcc ctccgggggc gcgccgtagg cgtcgaggag cgccgcgacg
    10921 acctccgggc cgacctcgta ccggtcgcgc gcgatcgagg ggccgaaggc ggcggagagg
    10981 tctctcgggg aaaccccgcg gacgtcgagc gcgtcgagcg ccgcgtcgag gacgcggagc
    11041 gccgtgcccc gccacccggc gtgaatggcg gcgatccatc cacgcacggg atcggcgagg
    11101 acgatcggca cgcagtcggc ggtggcgacg gcgagaagga cgttcggctg gcaggtgatc
    11161 gcggcgtcgc cttctccgag gaggaggttc cgccgctcac gaggcggttc ttcgaaggtg
    11221 agtgtgccgc ggccgtggac ctgggtgagg cggatcacct ccgcatccgg tgcgccgagg
    11281 tccttggcga gcgcgcggcc ggcgagatcc gggtcgaggg cgcgcgccgt ccgcgcgccg
    11341 cgtgtgaacc cggccgcgaa cccgggagca ccgggcaacg acagccgcga aaccccgcca
    11401 cccgaggtcg cgcctgaagt cgcgcgcgtc gccgggtgcc catccacatg ttcattctaa
    11461 gaaatcttct tctttctctt ttctttcccc ctctagaatc cccgcgtctt gaagaacgcg
    11521 acgcttcgcc tcccgcgcgg cctgcggtcg cccgccgcca tccagcgctt cctcgacggc
    11581 ctccgctaca acaaggaaga gggcggcgag acggcgcgct ccccgcgccg cgtcctcgag
    11641 acgggcgagg cgcactgttt cgagggcgcg ctcttcgcgg cggcggcgct ccgccggctc
    11701 gggcacccgc cgctcgtcgt ccagctgcgc gcggtgcgcg acgacgacca cgttctcgcg
    11761 ctcttccggg cggggtccgg cacggggtgc tggggcgcgg tcgcgaagtc gaactactcc
    11821 gggctgcgct tccgctcgcc ggtctaccgc tcgctccgcg aactcgcgat gagctatttc
    11881 gactgctact acaacctcga gggcgagaag agcctccgga gcttcgggcg gcccgtcgac
    11941 ctcgcgcgct tcgacgcgcg cggctgggag acggcggggg aggacctctg ggacgtctcg
    12001 actcacgtcg cgacgcgccg gttcactccc ctcgtgagcg ccgcgcaggc acggcgcctg
    12061 ccgccgatgg accggcgcct cttcgacgcc ggtctcgtcg gccggcgcgt ctagaaccgg
    12121 tagctgaccc gcgtgtaggc aaaggttccg ttgtacccgt acggggagtt gttcggatac
    12181 cggttgatgc cgctgttgga gttcacgaaa agggtcgaat ccggcgtcgc gtccccgagg
    12241 ttctgggcgc cgatcgcgac gcggagatgg ctgaacaccc aggagagctc gaggtcgagc
    12301 agccactgtg ccgagaacgt ctggtcggcg gccgtgcccg ggccgaacgg cgcctccccg
    12361 gagccgttcg cgatgccgca gaacgagccg tagcgcgtgg cgttgacgac gacgttcaga
    12421 ggccccttcg tgtagtcgac cgtccccctc acgacgtcct tcggctggcc acactcgagg
    12481 cggcggactt ccaggcggtc gaagagagtc gtctggaacg ccgcgagctg gggcggcgtc
    12541 gaggcgatgt tcgtcacgct cgtctccgcg tgcgtccacg cggcgagcag gcgcacggcg
    12601 ccgttcggtc ccaggtcggc gcggtagttc gccgtcaggt ccacgccgtc cgtgcgcgtg
    12661 tcgatcgcgt tcgtgaagaa cctcacgccg ctcgcgccga agggctggag gatcgggttc
    12721 agggccgcgc ccgtgaagtt gcccgagaag atgatccggt ccttgatcgc gatgttgaag
    12781 tagtccgccg tgaagtcgaa gtcgcggctc ggcgacagca cgaggccgcc cgcgaaattg
    12841 acggagtcct ccggcttcag gggcgtcgcg ccgagcgcct gggccacgcc gctgtcgacg
    12901 cgggccgtca ggatgtcgaa cggctggttg acgccgttga tgttcaggaa attcgtcgag
    12961 acggtcgaga agtaggactg gtgaagcgac ggggcgcgga agcccgtcga cgccgagccc
    13021 cgcgcgacga gccacgggag gacctcgtag cgaagggtgc ccttgtagtt cgtcgagttg
    13081 ccgaagtccg tgtagtgctc gaagcggacc gccgcgccga cgcggagctg cttcgtgacg
    13141 tcgacctcgg cgtcggcgta gagcgacacg ttgctgcggt tcgtgtcgat ctcgttcgcc
    13201 gggcggaagc cggggaagac ctgcgcgccc ggaggcgcag gcgagccgtc ctgcgccttg
    13261 acgccgccgt cgaagtacga ggcgggctcg ccggcgatct gccggtaacg ctcgttgcgg
    13321 aactcggcgc cgagtgcgag gttcagcggc ccggcgaggc cgatcgcgaa ctggcgcgtg
    13381 acgtcgaggt tcgcgaggaa ctgcccgaag acgagcgacc cggcgtcgaa ggaggtctgg
    13441 ttcggcggga tcgtcgggcc gagcgacgtg ttcagactgt ccacgacctt gaagtcgaac
    13501 tggttgcgtc cgtactggac cgacgcgtcc cagaaccagt gattcgcgag gacgccgcgc
    13561 acgcccgcgg tcgccgagta gtcgatcgtc gtcggcgtga tcttcgggag gaacccgttc
    13621 gggtagatcg tccgccagtt ctgggcctgc agcgcgcgcc ggaagttccc ggccgagtcc
    13681 gcgtagcggt ggctcgcgcc gccgaagagg taggcgttca ccgcgccgtc ggcgctcagg
    13741 gggaagacgc cgttcagaaa cccgagatag tccgtcgtcg cggggtctcc gtaccgcgtg
    13801 ttgggctgcg tgatcgggtc gcgggagccc tgcgcccgcg ggtcgtcgcc cgcgcggttc
    13861 gtcgcgttgc ggcggcggaa ctcgccggtg acgttgatcg agcccgagcc gaccgggaac
    13921 ccgccgttcg cgttgaggtc gaggaccgat ccgtcgccgt ggttcgtgat gccggtcgtg
    13981 gccgtgacgt cgagcggcgc cgggccggag cgcaacccga ggttcaggac gcccgcgatc
    14041 gcgtccgagc cgtactgcgc ggcggcacca tccttgagga cctcgatccg gtcgagcgcc
    14101 tgcgcgggga tcgcgttgag gtcgacgccg gccgagccgc gcccgatcgt cccgttcacg
    14161 ttcacgagcg cgctcgggtg gcgccgtttc ccgttcagga ggacgagaaa ctggtcgggc
    14221 ccgaggcttc ggatcgaggc aggccgggtc gtgtcggtcc cgtctgagat cgtcgtgcgc
    14281 gggaagttga acgacggctc gagcttctcg agaatctgga agacctcgcc cgagccgctc
    14341 gccttctcga tcgcggaagc gggaaacact tccacgggga ccgccttctc gacctcggcc
    14401 ccggccgcgc gcgaaccgac ggtcacctga gcgctcacgc cgagcgccat cggaaagtcc
    14461 tgcgcgacgg gcccgggtgc gagcgtgacc ttcgtggact tctccgcgag cccgggcgcc
    14521 gtgacgcgca cgtccaccgt gcggcctttc gcgatccctg ccggaatccg gatctcgtag
    14581 cggccgttcc ggtccgctct tgccacgagc tcgagggagg ggatcgccac ggtggcgccg
    14641 gggtacggct gtccatcctc cttcgtcgtg accgtgccgg acaggacggt tgccgagtcc
    14701 tgcgcctgag cagcggcgga ccccatcagg aatgcgattg ccaacgcaag gaagagcttc
    14761 atgagtctct cctctcggaa cggaaaagac agcgacaggc cggattgttg aggagactct
    14821 gtctcagata ataccgcggg tcaaggggcg gttggcgccc ccccagcccc cgcgatcgcg
    14881 ttcgcgtagt agcgcagcag cggaagctcc tccgggaccg ggcggtggag gccgccgtcg
    14941 gtgacgacga cgtggcgcag caggagcgcc ctcaggccca cggacaccgc gtagtcctgg
    15001 tcgccccgcg gcacgtggac gtaggcgccc cccgcttcga gggagcgcca gcgggcgagg
    15061 acctcggctt tgagctcgag ctcgctccac gcgcgctccg gggcgtccac gagcacgctc
    15121 gcgacgagcg agacgggaag gaccgggatc acgcgcgcga tccgctccat gaggtccttc
    15181 gcgaggtcct ccacggccgc gaaccgcgcc tcccgagaag cggccccgag gtcgaggttc
    15241 ctcgctgcga gccacgcccg cgtcgagacg ggagatccga agctcacgca ggcgtagccg
    15301 aagcggtgcc attcgttcgc gatcacgagg cgcgcgttgc gccggacgaa gcgcagcgtc
    15361 tcggagaggg tgtcggatgc cgaggccttg ccctgcgccg gggagcggtc gaggaggagc
    15421 gtgcggtcct cgaggacgcg gtcgtagttg acgcccacgg ggacgaagac gaggtctcgc
    15481 gggcccttcg ggtcgaagcc gcggagcatg tagtcgagga ggccgagctt cggcgggcgg
    15541 agcgcgccgt ccttcgagag gccaccctcc gggtagacgg cctgcgggac gccgttcgcg
    15601 gtcgccatcg cgacccagcg ctcgaggacg cgccgataga gcgggtcctt cgagtcgcgc
    15661 cggacgaagt acgcgccggt cgcccgcagg aacccctgga tgggccagat ccgcgcccat
    15721 tcgccgacgg cgtagctgag ggcggtcttc tcggccgcga ggtacgagag gaggaagtag
    15781 tccatgttgc tccggtggtt catgacgaag accaccgtgg agccctcggg aatcgcgcgg
    15841 aggccggcgt cgtccgccgt gccgaggcgc acgcggtaga gcgagcgcgc gaccgcgcgg
    15901 gcgatcgcgt acccggcgcg gaagtagatc cacgcgttga aggcggggac gatctcgcgg
    15961 gcgtcccgct cgatgcgttt ctgcacgagt tcgcggggct gaccggtctc tcgcgcgtac
    16021 tcctcggccg cctcgaggac tctcgggtcg aaccggagct ggtcgacgag gacctcgcgc
    16081 ttcgtcatct tgaagggcgg gaggcgcacg gggaggcgcg cgttcagctc gtcgatgaca
    16141 cggttcgcac ggcggcgcat cacggcgcgt gcggccgggc ggagcatccg gtcgaggacc
    16201 gcccagctcg cgaggaacgc gagcagcacg aacgcccaga gcgggaccgc gacgggggag
    16261 gacatcggag ttccttcgaa gattaaggca tgagcgccga atcgaggtct aatggcgcca
    16321 atggagagac gaatctgttt gttcggcgcg cgagccctgc tcctcgggct ctgtgcggcg
    16381 gcgcccgcag cgcgcgcgca ggtcgtcccg gtggagcccg tcccgaatga agccattctc
    16441 agggacttcc ggttctcgtc cggcgagacg ctcgcggagc tgaagatcaa ctacgtcacg
    16501 tacggccggc gcctcgacga cggcaggggg aagaccgcga acgccgtgct cgtcctgcac
    16561 gggacgggcg gctcgtcggc gcagttcctc ggcccgacgt tcgcgggcgt cctcttcggc
    16621 ccgggccagc cgctcgacgc ctcccgctac tacatcgtga tccccgacgg gatcggccac
    16681 ggcggctcct cgaagccgag cgacggcctg agagccaaat tcccgcggta cacgtacgac
    16741 gacatggtcc gcgcgcagca ccggctcctg accgaaagtc tcggcgtgac gcatctgcgc
    16801 ctcgtgatcg gcacgtcgat gggcgggatg cacgcgtggg tgtggggcga gacccacccc
    16861 gacttcatgg acgcgctcct gccgctcgcg agcctcccgg cgccgatcgc gggacgcaac
    16921 cggatgtggc ggaagatggc gatggacctg atccgcggcg atcccgcgtg gaaggggggc
    16981 gactacacgg agcagccgcc gggcctccgc ggcgcgctgt cgcttctgct cactctctcc
    17041 ggcagcccgc tcggctggat gcagcaggcg cccgaccggg ccgccgccga cgccgcggtg
    17101 ggcaagtaca tgtcggaacg gctcgcgaag acggacgcga acgacctcct cttcgcgctc
    17161 gacgcgtcgc gcgactacga tccgtggctc gggctcgaga agatcagggc gcccctcctc
    17221 gcgatcaact tcgcggacga cgcgatcaac ccgcccgagc tcggaatcct cgagaagggc
    17281 atcgcgcgcg tgaagaacgg gcaggccgtc gtctttccgc tctcggagaa gacgcgcggc
    17341 caccagacgc acacgctcgc ggacgtctgg aagacgtacc tgaacgaact cctggtccgg
    17401 tcgcgcccgt aggagccact cagtgcaggg cccgcgcaag gcgcgcggca ccctcggcga
    17461 agacgtcggg cggcggaagg aggctcacga cgagccaggc ctcggagggg aactcgtaga
    17521 agtagcccgg gtggacgagg acgtcgtgcg tctcgaggag gtcgaggacg atctcttcct
    17581 cgctccggac cgcggggacc ctcaggcacg cggcccatcc cgcggccacg ggcgcgacgg
    17641 tcacggcgcc acctgcgggg aaggcggtcc tgagcgcgcg ttcgttcttg aggacgcgcg
    17701 cccagatcgc ctccgcggcg cgacggctgg tctcgaggag ccgggggagg gcgagctgca
    17761 cgggcgtccc gacggagagg tacgtgtccg cgacccactc caggcgccgg agcgcgtccc
    17821 cgagcactgg ctcggcgccg ttcgcgagga tccagccgag cttgagctgg gggagcgccg
    17881 ccgacttcga gaggccgccg agggagaaca cgagcgcatc gctccgcacg gccgcgaccg
    17941 gaacgtcgtc gggccggtcg gagtaccggt agtcgacgaa gacttcgtcc gaaatcacgg
    18001 cgaagcccct ctccgccgcg agcgtgagga gcgcgtcgag ctcggccgcg ccgatcgagc
    18061 tgcccgtcgg gttgttcggg ttcacgacga cgacggccgc cgtgcgcgtc ccctcggctg
    18121 cgaggcgcgc gacctcgcgg gcgacggccg aagcatgcac gcggaagccg tcctcggcgg
    18181 cgagcgggta gcgcgcgagc gccacgctct cgagaccggc gagggcgtcg aggagcgggt
    18241 acgacggagc ggggacgagt accgcgtcgc cggggtcggc gaggaccttg aagagccagc
    18301 cgtaggcctc gctcgtcgag gccgtgagga cgagatgctc gggcggcgcg aggacgccgt
    18361 ggacggccgc gtaccagttg ctcacggcga ggcgagcgcg gcgcagaccc cgggggtccg
    18421 gctcgtagac gaggccgcgc ggatccgcga gcggcgcgag gaggccggcg tcgcccggag
    18481 catccaggaa gaggtcgacg ccgcccgagc gcagggcgac cgccgtcggg ttcgagactg
    18541 tcagatcgag atgcggccgc gtccgcgccg cgagggcgcg cgtgacgcgg ttctcctcgt
    18601 ggacgggcgg caacctcctc gaaaacaagc gggcggcctt cagcgccccg cgacgaggtc
    18661 ttccggcttc cacggcgacg gcgggccgcc gagccaccgg gagaaccagt ccaggtgcgc
    18721 ggcgtagtag agcgccatct cgaacgcgct cggccagtgg cccgcctttt cgaaaacgat
    18781 caggcgcgac gggacgccgc gggcctgaag gtccgtgaag aactcgagcg actgcgtgta
    18841 gggcacccgg tagtccttct cgcccgtgat cacgaggcac ggcgtcttga aggccgtcac
    18901 gaactccgag gggctttgcg ccttgtagag agcggggttc tcccacgggg cgcccttgag
    18961 gtcccagtgc gggaaccaga cttcctcggt cgaggagtac atcgcgcgca ggtcgtagac
    19021 gcccatcatc gaggcgagcg ccttgaagcg cgtcgtgcgg ccctcgagcc acatcatcgc
    19081 gtagccgccc cacgaccagc ccatcgcgcc catgcggtcc gcgtcgacgt acggaagctt
    19141 cgcgagggcg tccgtcacct tctcgatgtc cgtcatgacc ttgccggtcc agtctccgga
    19201 gatcgcggag gtgtacgcct ggccgaagcc cgtcgagccg tgcggattcg ggaacgcgac
    19261 gacgtagccc gcgcccgggt agacctgcca gtcgccgcgg tacgcgtcgg cccactgctg
    19321 ctgaggcccg ccgtggacgt tcaggatgag cgggtacttt ttcgccgggt cgaagcggtg
    19381 cggcttgacg atccagacgt ggacgggctt tccgccggcg ccgggcacgg tgatctcctc
    19441 ggccgggcgg atgtcgacct ccttctcgaa ggccgcattg tgcgtcgtga gacgcgttcc
    19501 ctccccgccc gtggcgacgc cgaggcgcca gagctcgagc gggctgccga tccggcggcg
    19561 cgccgcgacg gcgaacgagg cgtcgggcgc gagcgcccac gcgtcgagcg agcccacgga
    19621 cgagacggcg cggagcgcgc ccgtcgcgag gacgatctcg tagagcgggg tccgcccctt
    19681 cacttcagcc gtgaagaaga tccgcgtccc gtccttcgag aactcgaagt cgcggacgtt
    19741 gtcgtcgaac gtcgcggtga ggtcacgctc ggcccccgtg gcgcggtcgc gaacgatgat
    19801 ccggaaccgg tcggcctcgt agcgcggggt gagctggcgc ttgaacgcga ggaaccgtcc
    19861 gtcgggggag tagcgaggcg agccgtcgaa gcccttgttg gcggcggtga ggtttttcgg
    19921 ggcggagagc gcggccgcgt cgcctccggc gggcacggtc cagacgtccg cgttcgtcga
    19981 gaacgccgcg tccgcgtcgc gattagacga gaacgccagc tcctttccgt cgggcgagag
    20041 gtcgaagtcg cccccgcctc ccagggagaa gccgggagag tcgaagtcgc cgggcgtcag
    20101 gtcgcggatc gccttgcccg cgtccagctc ggcgaggtcg aggaggaaga cgtgcgtgcg
    20161 cgtgccgtct ttccaggaag tccagtggcg gtagaggagc gagtccgcga ggtgcgcctt
    20221 gatcttgttc gcgtcccggg cgtcggacgc cttcttgttg caggcggcgt cggcgccgca
    20281 ggcgggcagg acgtcggctg cgaacacgag aaagcggccg tcccgggaga acgccggccc
    20341 ggcgacgccg ccgggaaagc gggtcttctg ctcggcctcg ccgccggcga gaggcaggaa
    20401 gaagacctgt ggctcgcccg tgcgcgtcga gacgaaggcg agcgtcttcc cgtcggggga
    20461 gaagaaggga tgctcgtcgc gcgcgtcgcc ggcggtgatc ggacgggcgt tcgagccgtc
    20521 cgcgtcgacc cgccagagcg cgacggtcct cttcttcgcg gcccagtcgt tcgtcgtgac
    20581 ggagtagacg atcgtccttc cgtccggcga gatcgcgggc tcgcccacgc ccttcgtccg
    20641 gtagagatcc tcgagcgtga aggcgcggcc ggtgtcggcg gcgggcgcga atgatgcggc
    20701 gagcagaagc agggcgagcg cgaggggtgc ggaccgtttc agcatgcgtc gagtctatcg
    20761 gtccgcgccc ctcgggcggc ctcagctggt cagtccatcg cgcttcttga gaatctcgtc
    20821 gaggaaccag cggttcttct ggtgcacctg gacgaggcgg gtgtagaggt cggccgtccc
    20881 gatgtcgccg gcgtccgtcg ccacctcggc gtccgcgtgc atctccttga tgacgacgtc
    20941 gagcgccgca cgggcctcgg tcagcatctc gcgcatcccg agcacgccct ccgagggctt
    21001 cacggtggcc gcggggaggt agtcggccgg atcgccgatc ggggcgcctc cgatcatgat
    21061 cgcgcgctcg gcgagctcgt cgatcgaggc gtacgtctgg gccgcctgct cgtcgaggag
    21121 caggtggatg tcgcggaaca gcgggccgta gctgagccag tggtacttct tgtagttgag
    21181 gtacgtgacg agggcgttcg cctgctgacg ctggagcgtc gcgacgacgg cgtcggcgcc
    21241 gtgacccttc ttcttgttct tcttcgccat gagggacctc cgctattaat ttagactaag
    21301 tctaaagaat gtcaagggac tgaatgctac attcccgccc ccgacagggc agacaggaaa
    21361 ccaatggccg aacagtacat cttcacgatg cagaacctgc ggaaggtggt cccgccgcag
    21421 cgcgaaatcc tccgcggcat ctacctctcc ttcttcccgg gcgccaagat cggcatcctc
    21481 gggctcaacg gcgccggaaa atctaccgtc ctgcgcatca tggcgggcgt cgacaaggag
    21541 ttcacgggcg aggcgtaccc ggcggaggga acgcgcatcg ggtacctgcc gcaggatccg
    21601 cagctcgacc cgaagaagac cgtcctcgag aacgtcgagg agggcgtcgc gccgctcaag
    21661 gccctcctga agcggttcga cgagctgggc gagaagatgg gcgagagcct gcccgacgcc
    21721 gagatggaca aggtcatggc cgagtacggg aaggtgcagg acgcgatcga agccgcgaac
    21781 ggctgggagc tggaccgcac gctcgaaacg gccatggacg cgctgcgctg cccgccgccg
    21841 gacgcggacg tcacgaagat ctcgggcggc gagaagcgcc gcgtggcgct ctgccggctc
    21901 ctcctccaga aaaacgacat gctcctcctc gacgagccca cgaaccacct tgacgcggag
    21961 tccgtcgcgt ggctcgagca gtttctgaag gagttcccgg gcacggtcgt cgccgtcacg
    22021 cacgaccgct acttcctcga caacgtcgcc ggctggatcc tcgagctgga ccgcggcgcg
    22081 gggatccctt ggaagggcaa ctactcctcg tggctcgagc agaaggaggc gcgcctcgag
    22141 atcgaggaga agaccgagag cgcccgccag cgctccctca agcgcgagct cgagtgggtg
    22201 cgcatggcgc cgcgcgcgcg ccaggccaag agcaaagccc gcctgcaggc gtacgaacag
    22261 ctcgtctccg aaagcggcat cgaggcgcgc ggcgagacga acgaaatcta catcccgccg
    22321 ggcccgcgcc tcggcgacgt cgtcgtgcgg gccgagggcg tgaagaaggc ttacggcgac
    22381 aagctcctct tcgacgacct cagtttcgac ctgccgaagg gcggaatcgt cggcgtgatc
    22441 gggccgaacg gtgccggcaa gacgacgctc ttcaagatga tcatgggcct cgagaagccg
    22501 gatggcggga aactccaggt cggcgagacc gtcgtcccga gctacgtgga ccagaaccgc
    22561 gagaagcccg acgcgaagaa gaccgtgcac gacgagctgt cggagggaaa cgacctcgtc
    22621 atgctcggca agcgcgagat gccgagccgc gccttctgcg ccatgtacaa cttcaagggc
    22681 gccgatcagc agaagctcgt cgcgaacctg tcgggcggcg agatgaaccg ccttcacctc
    22741 gcgcggacgc tcaagagggg cgggaacctc ctcctcctcg acgagcccac gaacgacctc
    22801 gacgtcgaca cgctccgcgc gctggaggag gcactcctct ccttcggcgg ctgcgccgtc
    22861 gtgatcagcc acgaccgctg gttcctcgac cgcgtcgcga cgcacatgct cgcgttcgag
    22921 ggcgacagca agacggtttg gtacgagggc aactaccagg actacgaggc cgaccgccac
    22981 aagcgcctcg gcgccgaggc cgaccgcccg caccggatca agtaccgaaa gatcggctga
    23041 cgcccgcgcc gagacggcgt ctcaccttcg cggtaggatg cacggacaca tgtccgcatc
    23101 cgagacggcc gccgcggcgg ccgcgccgcg cctcgacgcg ccgcgcgtcg aggcgc
//
LOCUS       DAOMFY010000033        22043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_754_length_22043_cov_6.978079, whole genome shotgun sequence.
ACCESSION   DAOMFY010000033 DAOMFY010000000
VERSION     DAOMFY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22043
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_754_length_22043_cov_6.978079"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLB01_04730"
     CDS             <1..445
                     /locus_tag="PLB01_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76637.1"
                     /translation="PACARGSARIVDAGVPPLPRERFGAVLLDAPCSGTGTLRKNPEI
                     RLRLGPGDLAGFAATQRRLLDAALALLAPGGSLLYVTCSLEPEENEHVVDAALADRTD
                     VARVRPALDSSGPLAERTDVSGLVRVLPGATVDGFSAILMTRMRR"
     gene            511..798
                     /locus_tag="PLB01_04735"
     CDS             511..798
                     /locus_tag="PLB01_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQQ76638.1"
                     /translation="MAGKADIVEAIANDTSLTRKDAAAALDSVVNAITGILKKGERVA
                     LPGLGVFHVADRKARTGVNPRTKAAIRIGAAKVARFRAGKDLKELLNRKRK"
     gene            830..1540
                     /locus_tag="PLB01_04740"
     CDS             830..1540
                     /locus_tag="PLB01_04740"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQQ76639.1"
                     /translation="MIPLRDTIPSRTVPFLTRLLVVANVAAFVLEILQGDRLDAFVNT
                     FAFVPARFFHPEQFAGWTTGASVVTIFTAMFLHGGFLHLAGNMLFLWIFGDNVEDALG
                     HARFLIFYLACGASATLLQAFLSTASTIPNLGASGAIAGVLGAYFVLYPRARVVTVIP
                     LFVLFPLVEIPAGLYLLGWFLLQFWMGSSQLASVGRAGAEQGGVAFWAHVGGFVAGVA
                     WILLFRPKRPPAARYRIT"
     gene            1537..2886
                     /locus_tag="PLB01_04745"
     CDS             1537..2886
                     /locus_tag="PLB01_04745"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ76640.1"
                     /translation="MKSRGSTVTAWSLYELGATAFAMNLLSLHLPLDVAGRVPRGNEK
                     FSLAFGLSMAAVAVAAPFLGNLADRKGKRRLLVPFVLAGVACTALVAAPGPVAQVLVL
                     FALANVAFQCAYVFYNAMLPDVSDASNAGRVSGFGVAAGYVGSLLGMFLVLPFVSGSI
                     RAQMPDAIRAICDAISVAPVSSNVGDAWVRRNAYLPTALLWLAAAVPLLFFARLPRAA
                     SSREAARAPERASVPSSPSKRSSSSPFSEVLRTIRSLPKTPALLWFLVSNFLYVDVIH
                     TIQIQMATYSKFAVGLSDGQVQVLLLVATAVAVFGGLLYGWLCQRVTIRTATLVALFN
                     WVLVFALALLVRDPGAFTAVGVLAGVGLGGIKVTGRLGLVALVPKERMTEFFGFFTLA
                     GEAASVLGPFVWAGTLALFPDRSPAGYRAGVGVLFAVLALAIGTFLKVKFPRDAQAP"
     gene            2883..4229
                     /locus_tag="PLB01_04750"
     CDS             2883..4229
                     /locus_tag="PLB01_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQQ76641.1"
                     /translation="MKAAVFALVRALVGLFYRRIEVSGLEHVPREGPVLLIANHNNGL
                     VDPMVVLKALPRPVVFVAKSTLWKIPVLRSILHLLGCVPVVRKGEGEPGMSLKGEERN
                     SAAFERLASELTRGACVLIFPEGRSHSDPRLSELRTGAARVLLLSKKSPVVLPVGLWF
                     EEKEIFRSNVLVKVGAPVAPPADPTVEAWTDAMGAALGDVTLNADDWKDHEIVAAVDA
                     LYGEKIRGDFFEGEEGNAQMESSLRVRQLLLEARGALEKTHPGEVASLARRARALDHL
                     LRRISLSFSSLDDPPRASTILWNVLKALAVIVLGFPVAVVGVAAWWVPYRLCGFVANR
                     VPGAARERDQIALYKLLAGAVLFPLFLALWAAAAWYAAGPLAAPLSLAVLPFAGISSL
                     LFLEYAQWREQQARELLALLVAPGGIARLRAERDALVAEFDRLAAGFRPAADNPAR"
     gene            4226..5245
                     /locus_tag="PLB01_04755"
     CDS             4226..5245
                     /locus_tag="PLB01_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQQ76642.1"
                     /translation="MTVDVQASSTAPYERTSYVLAALALLAALLLHLVPALLAGFLAW
                     ALLQSMARRLRGGRVSHGLARALAAATLGLVAAGLVTLAIVLLWGFVRGRVGDLPAVL
                     EKMGETLARLRETLEASGVSLPLPAHGGSTEAVAGWLRENAGALRHAGSVGARGLLHA
                     LLGSVVGVLVFFHGPAESPGPLAAALSERLRRFGECFRSVAKAQIEISAVNTALTAVF
                     LFAAEPLFGVRLPLPGTLVAITFLCGLVPVAGNLVSNTVIVIVSLGVSPWAGVAALAF
                     LLVIHKLEYFLNAKIVGGRIGASAWETLLAILVMEAAFGMPGVVLAPVVYAWAKSELV
                     SRGLV"
     gene            5270..6430
                     /locus_tag="PLB01_04760"
     CDS             5270..6430
                     /locus_tag="PLB01_04760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQQ76643.1"
                     /translation="MTTISAPPATPVPLLDLKKQYATLKDEMLRVTAEVYESQGFILG
                     PRVEAFEKAVAAYVGARHAIGMSSGTDAQLAVMMALGVGPGDDVVTSPYTFFASAGAV
                     ARLGARPVFVDIDPDTFNLDPAKLEKALTAKTKAIQPVHLYGQCADMDPIREIAKKKG
                     IHVLEDACQSLGAGYKGVKAGALGDSCAFSFFPSKNLGGFGDGGMVTTSDDALAANLK
                     AMRMHGETSRYHHKFVGGNFRLDALQAAVLHVKLPHLDGWAEARRANSKDIERRYLEA
                     GGLPFERGGLKFPHEAAGRYHVFNQFVVRVGGGRRDSLKDQLAAKGIGCAIYYPVPLH
                     LQECFASLGRKAGDFPESERAAKETLALPVFPELEDAQKAFLAATLAEFSKG"
     gene            6638..6931
                     /locus_tag="PLB01_04765"
     CDS             6638..6931
                     /locus_tag="PLB01_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQQ76644.1"
                     /translation="MKVRPLYDRILVKRVEPKEQVRGGIIIPDTAKEKPMEAKVEAVG
                     EGKFDDNGKRVPLDVKKGDRILIGKYAGTEIKIEETEYLILREDEVLAIVETK"
     gene            6948..8591
                     /gene="groL"
                     /locus_tag="PLB01_04770"
     CDS             6948..8591
                     /gene="groL"
                     /locus_tag="PLB01_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527640.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQQ76645.1"
                     /translation="MAAKNITYGEDARQNILRGVNKLADAVKVTLGPKGRNVVIDKKF
                     GSPTITKDGVTVAKEIELDNALENMGAQMVREVASKTSDIAGDGTTTATVLAQAIYRE
                     GIKMVTAGGNPMEIKRGIDLAVKKAVDSINAMKKTVDAKEIAHVGTISANGDVEIGGI
                     IAQAMDKVGKDGVITVEEAKGLDTTLEVVEGMQFDRGYLSPYFVTDAERMEGVLEGAK
                     ILIYEKKISSMKDLLPILEQVAKQNRPFLIIAEEVEGEALATLVVNKLRGTLHVAAVK
                     APGFGDRRKAMLEDIAILTGGKMISEDLGIKLESVKWEDLGEAKKVTVDKDNTTIVVD
                     TGDKKRREAITGRVKQIRAQIEETTSDYDREKLQERLAKLVGGVAIIKVGAATETEMK
                     EKKARVEDAMHATKAAVEDGIVPGGGVALLRAIAEVDKLKEHGDIQIGINIVKRALEE
                     PARQIIANAGLEGSVIVKELKEKGGFIGFNAATEKTEDMLKAGIVDPAKVTKSALQNA
                     ASIAGLMLTTEAMVSEIKEKEKAPAGGGMGGGGGMGGMY"
     gene            8803..9153
                     /locus_tag="PLB01_04775"
     CDS             8803..9153
                     /locus_tag="PLB01_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76646.1"
                     /translation="MYFLRESSSSSSLSLLSLVFLAAAGTAAAQSNQTPNLDRREAQQ
                     QQRIAQGIQSGQLTPAEASRLEQREAKLNADEARAKSDGVVTKKERAHLQNEANRDSN
                     AITRKKHNARVAPH"
     gene            9211..10929
                     /locus_tag="PLB01_04780"
     CDS             9211..10929
                     /locus_tag="PLB01_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76647.1"
                     /translation="MSERNAVAAPLRLGLAAGVVFAPLAAVLALGAGAASLWLDEITY
                     ARLQSDFALRAAEIGRSGSAVAPYFSNFFYCDVQKVFQSLLAATGLVRFESSPEWLVR
                     SLSVAAYAATVVLVGVRARRRELPGSGTLGAFLFAAAPVFLYYAFEARVYALVSFLAV
                     LLLERIEKTAARVTVPAILAVAFLGVVVARLHLWTLCLFAALFARAAVEVVRRRRATP
                     LARVLLAASVPALATIFLEYGFMKATQPPEPLFALFARQPLGGTLLQTGLSIFEGPLQ
                     VQSAFQEPLTRAFVAGCLVLLGVLVIGALREPEAEGAFGARSAAAVALFALAISVALA
                     VGFGYFVHGRYQVPLFAVLFWAVARSLTNRRRLLLALLFAAAEAVLLPSTAAAIRAKS
                     NDAEIAGVIAKGGDRSTTAVVVQHGVISGYPAPHHTIGLDFYLNDVHPGGARIPLFEL
                     PDLRAVQGDHGTYRYFNGGAPLLSRILEIRPEVFRTWADREAPADVWVVQPLWDVARS
                     REQIAAFLEVLVGERRYAVAGAFVADGYPRARVIHLRRGPQAPGAADSGPGHAANAMV
                     GEQTRQ"
     gene            complement(10876..11766)
                     /locus_tag="PLB01_04785"
     CDS             complement(10876..11766)
                     /locus_tag="PLB01_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ76648.1"
                     /translation="MTSAGGLVPLAPPARRDVGLGTAYERWAVYELLGKWFTDETPAT
                     ALEGPVDGMAGIPGLHLLPAAARGTRVTVIVPDAEGAEVVRGVYRTVGLEDRLDARAG
                     VAWPDETFDLVLTYNALPSVPDWKRYLAEAAARARRQLVVSVTHPASYGVFLRKALRL
                     VQPGEKPLELFDHPSTSRRTLEPELRTHGRITGDAFVDCPWWPDLFVETGETLFTGTL
                     SRLPFGSRFRRGPASAAPATPGEEPFLYGPGRFPFLGGAGWREELEPALRRHPNLDGG
                     RFPSLAGLLAHHRVRRVTRP"
     gene            complement(11763..12722)
                     /locus_tag="PLB01_04790"
     CDS             complement(11763..12722)
                     /locus_tag="PLB01_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ76649.1"
                     /translation="MTVAVTGATGFIGGHVARTLAARGVPVIALHRHATPPAPRPGIT
                     WTTFEDFETGGEPVDALVHVAAVRHRHGVPPEEYGRQNVALTARFLAAAKGRVGRFVD
                     VSSIAVFGWPPDPPIDETRPFAPVGPYGRSKVETERMVRESGLPYAIVRPSITYGPGD
                     TNGMIDKLFRLVKAGRYRIVGSGSSRVQLVYAEDLANAIAETALRPGLDGAEFICTYR
                     EPITIRALSTLTADIAGRSLPALGVPLGVARAAGIAFETLEKLGVRFPGGEPPVTREK
                     LQTVTVDRAYRIDRMRGLLGWEPPTSYETGLRLTAAALAEAGA"
     gene            complement(12719..13471)
                     /locus_tag="PLB01_04795"
     CDS             complement(12719..13471)
                     /locus_tag="PLB01_04795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ76650.1"
                     /translation="MTPRHALVIAPHGAVGNAVAGELRRRGARVTGAGRTPPDPGVVD
                     SFLPLDLASADWPRLLAEASGDGPLDAIVYAAGSGAFGLAGSIPEDQGRAVFEINFWA
                     LTEAARAAAARWTRDGTPGTFLAVLSIAGRRAVPFESYYGASKAAALRFLEVLDLETP
                     ESIRFVAAAPGLIRSPFRSRAAWHGMAEPPATGGASPGETAVALCALLEGSRESRVIG
                     WRERAIDLADRFAPGLYDRLVLRPRIARGHRP"
     gene            complement(13468..14397)
                     /locus_tag="PLB01_04800"
     CDS             complement(13468..14397)
                     /locus_tag="PLB01_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ76651.1"
                     /translation="MTTVVVGADGFVGGALASALGAAPVVFRAPRTSAEIPVEDAGAV
                     LAAARVVVNASGVRVRPGLGPADYRRTHAEAVQRLVARLSPGALLVQVSSASVLGRSP
                     AASPRNDEAGSPETFGCPDYAVAERDAERAARAAAAARGVCLAILRPAILYGREPDGM
                     LGTLGAIAGHGLLLRLAPARHRHHLCALPLLVEAVRTVARRGEAIEPPLTVADPFFVT
                     SGEVAAIGRELHRPRFSLPFPAGAAGEVLRRLPRSSSPRLDLRTWGEILCILALDTVY
                     DPSETYRLLEIDASRFSRARTWERLARGLEAET"
     gene            complement(14394..15389)
                     /locus_tag="PLB01_04805"
     CDS             complement(14394..15389)
                     /locus_tag="PLB01_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQQ76652.1"
                     /translation="MRARARTWGLALLAGVAAFALWWRLFDGPRVASLALRASPGLVL
                     AGFALMGAAQFLRLLRWHALLNRLGRVPLPRTFHLLYASELLNNVLPVKVGDLGRAVA
                     LAGRSPGFTVGSAAASMVVDRLWGIATRLLALPLLLVIPSRLPASLRVSIGIFAALLV
                     LAVAALFVWGRHGETLARTGAPLLRFVPAPARDATARTLRAFADSSVEVGMRPGLAAA
                     LAALSAGSLLAQAAGLALLFRAAGHPVPFAVAVAGTALLDLLAVVPAPPAGVGTTEWY
                     ATLIFATALGQPVDATAAAALLYHAAWLLIVFVAGAASLPAVAEMLPRRAPETPA"
     gene            complement(15386..16288)
                     /locus_tag="PLB01_04810"
     CDS             complement(15386..16288)
                     /locus_tag="PLB01_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ76653.1"
                     /translation="MRVVAGVTACNEEQTIGPLLEALLAARPFDAPIEAVTVVSSACR
                     DRTDEIVRDFAARDARVALIAEAERRGKAAAINSFLAARPPGTDVTIIASADVLPEPG
                     ATERIVAAFADPAVGMAGGRPVPRNEGRALLDRMARLMWHLHDCVARRSPKLGELVAV
                     RSALVESLDAASPVDESSLEAAVRERGGRLAYVPDAVIGNRGPATYREWMSQRRRIAF
                     GHLWLKHRTGYRVSTGSGASVLPIWLAEVLPHPSRWLPGLALAGTEALARLEAWRDYR
                     GGEKNYTVWSIAASTKAGSGAPPS"
     gene            complement(16299..18389)
                     /locus_tag="PLB01_04815"
     CDS             complement(16299..18389)
                     /locus_tag="PLB01_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76654.1"
                     /translation="MTDLKPEPPFVSRDDVDLGTVSFAVLFLELSLIRWISGYIINFG
                     YFTNFILLGAFLGIGLGCLAARRTETLIRYFTAILLATTGIVYAFQVRIIELPATRSV
                     VFWAEAPSSDIHRTAIPTYLLVPILFALTTLLFAALATPMGVLFGRLPRLRAYTVNIV
                     GSLLGIGAFALCSLLWAPPVVWFGVCLLASVPVLAGTPRRGWALQGLAAAGALGIVAM
                     MGRGDTWGPYYRQNLKKIDDKTFLLAGNGVYGIGIHEPSESAIYHHLYDSPYGEILAP
                     FRDAPPRYRSALVIGCGAGNETAYAIRHGVDEIDAVDINPWVIEIGRQFHPEKPFSSP
                     RVTTHVADGRAFIRHSTRKYDLIVYGLPDSTFNLSDRTNLRMESFLFTVEAFREIRAH
                     LTEHGVFVLYNYYRLPWVVQKIEGMLREAFDRDPAVFRVQTERGGWGFPAILAAGPGL
                     PHLASRKPPAPNEPTLARDDWPFVYLFEPNVPPHYLVSLGIVALVSLLSVVLGLRLAG
                     PSPPGERELPGHLAAFFFMGAAFLLLETRSVATFGLFFGSTWATNAVVFAAIHVSVLL
                     AIWVAARFPRIPRLGLFGALSASLLVSWVVPLDALLTRDYFWRALVVSSVAFLPIFCA
                     NVLFATFFRNAREGAKSYALNLLGGMLGGMLEYSSLLLGYRALIGLAGVLYLLALLAV
                     LRAESGSAAATAPA"
     gene            complement(18396..20039)
                     /locus_tag="PLB01_04820"
     CDS             complement(18396..20039)
                     /locus_tag="PLB01_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76655.1"
                     /translation="MTREDRPPRRAAANVAAFGLALVPAVLCISFGAFRAPLWLDEIL
                     YFYLQDDFALRAAEIGRPASSLAPYFGNFFFCDLQRLFQGLLGPLGLSVQSHPEATLR
                     LLPLAAFLGACAIFYRSFLRQSGRLDAVLGTLAFSSMPLVLHYAFEARVYVFTAFLVV
                     LLLSLLPRADEGVVPGRTVALAALGLVTAHSHLWTVCLFAALAADGALRLARSRGPTP
                     QALARLAASLPALGLIVAEYAFMKFTDPGRPQYPPFRPQPLRLTLEELFVSNFSGPLH
                     LQYYVFNQPSGKILVWAGAFLLVAIAILAWRHGDRERRRDILTAAAALALCAFLAVTF
                     GFFQHARYHLPLLAALLFAFGPPRGSVPRGLLAGLVLVNLLLLPDNLEEMDRKSDGRR
                     LAAHVARRFPDRSRVGVVFQNVPTGGYPFPSHSIAADFYMNFLHPNERPVSLYELPEL
                     NSVNGRRGVYPFFASDPSLLARTLKSRPEIWRSRLPLLPANLVLVNPIWGLEESQIQL
                     ADFLKIVRESPYRRSGSSFMMFGFPHLFMIELPAAPEGK"
     gene            complement(20036..21130)
                     /locus_tag="PLB01_04825"
     CDS             complement(20036..21130)
                     /locus_tag="PLB01_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ76656.1"
                     /translation="MSAIDRAPLLLKLLAFRSFHRFGSPRLLPINLTVSVTYTCPSRC
                     KTCDIWQKKVPDFTLEEYEKTFRSMEGVPIWVTVSGGDQFVRSDLPELFGLIRRILRP
                     KIINLPMNGLLTKQIEKQMPGIVEATKGSKLIVNLSLDEVGTRHDEIRGARDNWEKMM
                     KARALVLELQRKNPHVTVGIHTVVSSQNVARIPEIHAELKKLNPDSYITEVAEERVEL
                     GTMGKDITPSAAEYAAAADYLIAEMRRNRLRHPMGRLVESFRLEYYELVKEYLERREQ
                     IIPCYAGWASAQIAPDGYVWGCCIRAESVGGLRENGYDFGKVWFSPEAGRFRKSVYNR
                     ECACPLANASYTNMLVSPRSLVRVARNWIA"
     gene            complement(21127..21957)
                     /locus_tag="PLB01_04830"
     CDS             complement(21127..21957)
                     /locus_tag="PLB01_04830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ76657.1"
                     /translation="MDLSAPFSQERLDRVGTLSRETLVVSPTFREAETIADHVAAVFH
                     VLPEVTLLIVDDESPDGTADAVEALRARHPRLHLLRRKPPRSFTGSYKDGISWAIEHG
                     FSRVVSMDADGSHAADFLPLLIDMSHDADLVVGSRYLYGVSVLNWPMRRVFLSAFASV
                     YSRLITGVPCDDLTSGFCCYRVDMLEKIGYRDIRSSGYAYQIEMKYRIWRASGQLAET
                     NILFVERRKGTSKISSGRVLEGVARPFWCRLFLPGGPADPKTPIKRGWESWHEWGSPL
                     "
BASE COUNT         3407 a         7441 c         7963 g         3232 t
ORIGIN      
        1 gccggcatgc gcgcggggaa gcgcccgcat cgtcgatgcg ggcgtcccgc cgcttccacg
       61 ggaacggttc ggcgccgtcc ttctcgacgc gccgtgctcg ggcacgggga cgctgcgcaa
      121 gaacccggag atccggctcc gcctcggccc gggcgacctc gcggggttcg ccgcgaccca
      181 gcgccgcctg ctcgacgcag ccctggcgct cctcgcgccg gggggctcgc tcctctacgt
      241 gacgtgctcg ctcgagcccg aggagaacga gcacgtcgtg gacgcggccc tggccgaccg
      301 aacggacgtg gcgcgggtcc ggcccgcctt ggattcgtcg gggccgctcg cggagcggac
      361 ggacgtgtcc gggctcgtgc gcgtcctgcc gggcgcgacg gtcgacgggt tcagcgccat
      421 cctcatgacg cgcatgcgtc gttgacaagg gaaaccgcgc gccctagatt gccggcgatt
      481 ttcgttctcc ggaatcgcag gaggatcccc atggcgggca aagccgacat cgtggaagcg
      541 atcgccaacg acacgagcct cacgcgcaag gacgccgcgg cggctctcga ctcggtcgtc
      601 aacgcgatca cggggatcct caagaagggc gagcgcgtgg cgctcccggg cctcggcgtc
      661 ttccacgtgg ccgaccgcaa ggcccgcacg ggcgtcaacc cgcggacgaa ggcggcgatc
      721 cggatcggcg cggccaaggt cgcgcgcttc cgcgccggca aggacctcaa ggagctcctc
      781 aaccgcaagc ggaagtagga gcccggcgcg cccgtatgat gggcgcctct tgattccgct
      841 tcgcgacacg atcccgtcca ggacggttcc gttcttgacg cgcctgctcg tcgtcgcgaa
      901 cgtcgcggcc ttcgtcctcg agattctcca gggggaccgc ctcgacgcgt tcgtgaacac
      961 cttcgcgttc gtcccggcga ggttcttcca tcccgagcag ttcgcggggt ggacgaccgg
     1021 cgcttccgtc gtgacgatct tcacggcgat gttcctccac ggcggattcc tgcacctcgc
     1081 cgggaacatg ctcttcctct ggatcttcgg cgacaacgtc gaggacgccc tcgggcacgc
     1141 gcgtttcctg atcttctacc tcgcgtgcgg cgccagcgcg acgcttctgc aggcgttcct
     1201 ctcgaccgcg tccacgatcc cgaacctcgg cgcctccggc gcgatcgcgg gcgtgctcgg
     1261 cgcgtacttc gttctctatc cgcgggcgcg cgtcgtcacg gtgattccgc tcttcgtcct
     1321 ctttccgctc gtcgagatcc ccgcgggcct ctatctcctg ggctggttcc tgctgcagtt
     1381 ctggatgggc tcctcacagc tcgcgtcggt cggccgcgcg ggagccgagc agggcggcgt
     1441 ggcgttctgg gcgcacgtcg ggggcttcgt cgcgggcgtg gcgtggattc tgctcttccg
     1501 cccgaaaagg ccgccggcgg cgcgataccg catcacgtga agagccgcgg gtcgacggtc
     1561 accgcctgga gcctctacga gctcggcgcg acggcgttcg cgatgaacct cctctcgctc
     1621 cacctgccgc tcgacgtcgc gggacgggtt ccgcgcggga acgagaagtt ctcgctcgcg
     1681 ttcgggctct cgatggccgc cgtcgcggtg gcggcgccgt ttctcgggaa cctcgcggac
     1741 cgcaagggca agcgccggct cctcgtgccg ttcgtcctcg cgggcgtcgc gtgcacggcc
     1801 ctcgtcgcgg cgccggggcc cgtcgcgcag gtcctcgttc tcttcgcgct cgcgaacgtc
     1861 gcgttccagt gcgcgtatgt cttctacaac gcgatgctgc cggacgtgtc ggacgcgtcg
     1921 aacgcagggc gcgtgtcggg gttcggcgtg gccgcggggt acgtcgggtc gctccttgga
     1981 atgtttctcg tcctgccgtt cgtttcgggg agcattcgcg cgcagatgcc cgacgcgatt
     2041 cgggcgatct gcgacgcgat ttcggtcgct cctgtctctt cgaatgtggg tgacgcctgg
     2101 gtgaggcgga atgcctacct gccgacggcg ctgctgtggc tcgccgcggc cgtcccgctg
     2161 ctgttcttcg cgcgcctgcc gagggcggcc tccagccgcg aggccgcgcg cgccccagag
     2221 cgcgcatcgg ttccctcttc accttctaag agatcttcct cttctccctt ctcagaagtc
     2281 ctccgaacca tccgctcatt gccgaagacg cccgcgctgc tctggtttct cgtctcgaac
     2341 ttcctctacg tcgacgtcat ccacacgatc cagatccaga tggcgacgta ctcgaagttc
     2401 gccgtgggcc tttcggacgg gcaggtgcag gtgctgctcc tcgtcgcgac ggccgtcgcc
     2461 gtcttcggag gcctgctcta cggctggctc tgccagcgcg tcacgatccg cacggcgacg
     2521 ctcgtcgcgc tgttcaactg ggtgctcgtg ttcgcactcg cgctgctcgt ccgcgacccg
     2581 ggggcgttca cggccgtcgg agtcctcgcc ggcgtcggcc tcggcggcat caaggtcacc
     2641 gggcgcctcg gcctcgtcgc gctcgttccg aaggagcgca tgacggagtt cttcggcttc
     2701 ttcacgctcg cgggcgaggc ggcgtcggtt ctcggcccgt tcgtatgggc gggaacgctc
     2761 gcgctgttcc cggaccggag tccggcgggc taccgcgccg gcgtcggcgt cctcttcgcg
     2821 gtcctcgcgc tcgcgatcgg gacgttcctg aaggtgaagt tcccgcgcga cgcgcaggcg
     2881 ccgtgaaggc cgccgttttc gcgctcgtcc gcgccctcgt cgggctcttc taccggcgga
     2941 tcgaggtgtc ggggctggaa cacgtcccgc gcgaggggcc ggtcctcctg atcgcgaacc
     3001 acaacaacgg cctcgtggat cccatggtcg tcctgaaggc ccttccgcgc cccgtcgttt
     3061 tcgtcgcgaa gtccacgctc tggaagatcc cggtgctccg gtcgatcctc cacctcctcg
     3121 gctgcgtgcc ggtcgtcagg aagggagagg gggaaccggg gatgtctttg aaaggggaag
     3181 agaggaacag cgcagccttc gaaaggctcg cctccgaact gacgcgaggc gcatgcgtcc
     3241 tgatcttccc tgagggccga tcccactcgg atccccgact ctccgaattg cgcaccggcg
     3301 ccgcccgcgt cctcctgctt tcgaagaaat ccccggtcgt ccttcccgtc ggcctctggt
     3361 tcgaggagaa agagatcttc cgttccaacg tcctcgtgaa ggtgggcgcg cccgttgcgc
     3421 cgccggccga tccgacggtg gaggcgtgga cggacgcgat gggcgccgcg ctcggggacg
     3481 tgacgctgaa cgcggacgac tggaaggacc atgaaatcgt cgcggcggtg gatgcgctct
     3541 acggggagaa gatccgagga gatttcttcg aaggtgaaga ggggaatgcg cagatggaga
     3601 gctcgttgcg cgtccgacag ctgctgctgg aggcgcgcgg tgcgctggag aagactcatc
     3661 ccggcgaggt ggcatccctc gcgcgtcgcg cccgcgccct cgaccacctt ctacggagaa
     3721 tctctctgtc tttctcttct ctcgacgacc cgcccagggc ctcgacgatc ctgtggaatg
     3781 tgctcaaggc cctcgccgtg atcgtcctcg ggttccccgt cgccgtcgtc ggcgtcgccg
     3841 cctggtgggt tccgtaccgc ctgtgcggat tcgtcgcgaa ccgcgtgccc ggcgccgcga
     3901 gggagcgcga ccagatcgcg ctctacaagc tcctcgcggg cgccgtcctt ttccctctct
     3961 ttctggccct ctgggccgcg gcggcctggt acgcagccgg cccgctcgcc gcgccgctca
     4021 gccttgccgt tcttcctttc gcaggaatct cttctcttct cttcctcgag tacgcccagt
     4081 ggcgcgagca gcaggcgcgg gagctgctgg cgctcctcgt cgcgccgggc gggatcgccc
     4141 ggctccgggc cgagcgcgac gcgctcgtgg cggagttcga ccggctcgcc gccggcttcc
     4201 ggcccgcggc ggataatccg gcccgatgac cgtcgacgtg caggcctctt cgacggctcc
     4261 gtacgagcgg acgtcgtacg tgctcgcggc gctcgcgctc ctcgccgcgc tgctcctgca
     4321 cctcgtcccg gcgctcctcg ccggatttct cgcctgggcg ctcctgcagt cgatggcgcg
     4381 gcgcctgcgc ggcgggcgcg tctcgcacgg ccttgcccgc gcgctcgccg cggccaccct
     4441 cggcctcgtc gcagcgggcc tcgtgacgct cgcgatcgtc cttctgtggg gcttcgtccg
     4501 cggccgggtc ggggatctgc cggcggtcct cgagaagatg ggcgagacgc tcgcgcgcct
     4561 gagggagacg ctcgaggcct cgggcgtgtc gctcccgctc ccggcgcacg gcgggtccac
     4621 ggaggcggtc gccggctggt tgcgcgagaa cgcaggcgcg cttcgccacg cgggctcggt
     4681 gggcgcgcgc gggctcctgc acgcgcttct cggttccgtg gtcggcgtcc tcgtgttctt
     4741 ccacgggcct gcagagagcc cgggcccgct tgcggccgcg ctctccgagc gcctccggcg
     4801 cttcggcgag tgcttccggt ccgtcgcgaa ggcgcagatc gagatttcgg ccgtgaacac
     4861 ggcgttgacg gccgtcttcc tcttcgcggc cgagccgctc ttcggcgtac gcttgcctct
     4921 tcccgggacg ctcgtcgcga tcacgttcct gtgcgggctc gtcccggtcg ccgggaacct
     4981 cgtttccaac accgtgatcg tcatcgtctc gctcggggtc tcgccgtggg cgggcgtggc
     5041 ggcgctcgcg ttccttctcg tgatccacaa gctcgaatac ttcctgaacg cgaagatcgt
     5101 gggtgggcgc atcggcgcgt ccgcatggga gacgctcctc gcgatcctcg tcatggaggc
     5161 ggcgttcggc atgccgggcg tcgtcctggc gccggtcgtc tacgcgtggg ccaagtccga
     5221 gctggtgagt cgcgggctcg tctaggcccg tctggtgtag cgtagacgcg tgacgacgat
     5281 cagcgcacct cctgccaccc ccgtccccct cctcgacctc aagaagcagt acgccaccct
     5341 gaaggacgag atgctccgcg tgaccgcgga ggtctacgaa agccagggct tcatcctcgg
     5401 tccccgcgtc gaggccttcg agaaggccgt cgccgcctac gtcggcgccc ggcacgcgat
     5461 cggcatgtcg tccggcaccg acgcgcagct cgcggtcatg atggcgctcg gggtcgggcc
     5521 gggcgacgac gtcgtgacgt cgccctacac gttcttcgca tccgcgggcg ccgtcgcgcg
     5581 cctcggggcg cggcccgtct tcgtcgacat cgaccccgac acgttcaacc tcgacccggc
     5641 gaagctcgag aaggccctga ccgcgaagac gaaggcgatc cagcccgttc acctctacgg
     5701 ccagtgcgcg gacatggacc cgatccggga gatcgcgaag aagaagggga tccacgtcct
     5761 cgaggacgcg tgccagtccc tcggcgccgg gtacaagggc gtgaaggccg gcgcgctcgg
     5821 cgattcctgc gcgttctcgt tcttcccgtc caagaacctc ggcggcttcg gcgacggcgg
     5881 catggtcacg acgtccgacg acgcgctcgc cgcgaacctc aaggcgatgc gcatgcacgg
     5941 cgagacgagc cgctatcacc acaagttcgt gggcggcaac ttccgcctcg atgcgctcca
     6001 ggccgcggtc ctccacgtga agctgccgca cctcgacggc tgggccgagg cccggcgggc
     6061 gaactcgaag gacatcgagc ggcgctacct cgaggccggc ggcctcccgt tcgagagggg
     6121 cggcctgaag ttcccgcacg aggccgcggg ccggtaccac gtcttcaacc agttcgtggt
     6181 tcgggtgggc ggggggagaa gagattcttt gaaggatcag ctggcggcca agggcatcgg
     6241 ctgcgccatc tactacccgg tgccgctgca cctgcaggag tgctttgcgt cgctgggtcg
     6301 caaggcgggc gacttccctg agtccgagcg ggccgcgaag gaaaccctcg cgttgcccgt
     6361 cttcccggaa ctcgaggatg ctcagaaggc gtttctggca gccacccttg ccgagttctc
     6421 gaagggctga acgcgcctcg atcggccctt agaataccct ccaacaaact tagattcaac
     6481 agtttacata gaatctaaca ggatccttga cagatcctgt cttcgtgcat atcgtgctta
     6541 gcactcgtgg cgcggctctg ctaacaggac ccccgcgagc atccgaatca agtcgagggc
     6601 atcgccctca ccgtctctca atggaaagga gtcactcatg aaggtccgtc ccctgtacga
     6661 ccggattctg gtcaagcgcg tcgagcccaa ggagcaggtt cgcggcggga tcatcattcc
     6721 cgacacggcc aaggagaagc cgatggaggc caaggtcgag gccgtcggcg aaggcaagtt
     6781 cgacgacaac ggcaagcgcg ttcccctcga cgtcaagaag ggcgaccgca tcctcatcgg
     6841 caagtacgcc ggcaccgaga tcaagatcga agagaccgag tacctgatcc tccgtgagga
     6901 cgaggttctc gcgatcgtcg agacgaagta agggagacac acacaccatg gctgccaaga
     6961 acatcaccta cggcgaagac gcccgccaga acatcctccg cggcgtcaac aagctggccg
     7021 acgcggtcaa ggtcacgctc gggcccaagg gccggaacgt cgtcatcgac aagaagttcg
     7081 gctcgccgac gatcacgaaa gacggcgtca cggtcgccaa ggagatcgag ctcgacaacg
     7141 cgctcgagaa catgggcgcc cagatggtcc gcgaggtcgc ctccaagacc tcggacatcg
     7201 ccggcgacgg cacgacgacg gccacggtcc tcgcgcaggc gatctaccgc gagggcatca
     7261 agatggtcac cgcgggcggc aacccgatgg agatcaagcg cggcatcgac ctcgccgtca
     7321 agaaggcggt cgattcgatc aacgcgatga agaagaccgt cgacgccaag gagatcgccc
     7381 acgtcggcac gatctcggcg aacggcgacg tggagatcgg cggcatcatc gcgcaggcga
     7441 tggacaaggt cggcaaggac ggcgtcatca cggtcgagga ggccaagggc ctcgacacga
     7501 ccctcgaggt cgtcgagggc atgcagttcg accgcggcta cctctcgccg tacttcgtca
     7561 cggatgccga gcgcatggaa ggcgtcctcg agggcgcgaa gatcctcatc tacgagaaga
     7621 agatctcgtc catgaaggac cttctcccga tcctcgagca ggtcgccaag cagaaccgtc
     7681 ccttcctcat catcgcggaa gaggtcgagg gcgaggcgct cgcgacgctc gtcgtgaaca
     7741 agctccgcgg cacgctgcac gtcgccgccg tcaaggcccc cggcttcggc gaccgccgca
     7801 aggccatgct cgaggacatc gcgatcctca cgggcggcaa gatgatctcc gaggacctcg
     7861 ggatcaagct cgagtccgtg aagtgggagg acctcggcga ggccaagaag gtcacggtcg
     7921 acaaggacaa cacgacgatc gtggtcgaca cgggcgacaa gaagcgccgc gaggcgatca
     7981 cgggccgcgt gaagcagatc cgggcgcaga tcgaggagac gacctcggac tacgaccgcg
     8041 agaagctcca ggagcggctc gcgaagctcg tcggcggcgt cgcgatcatc aaggtcggcg
     8101 cggccaccga gaccgagatg aaggagaaga aggcccgcgt ggaggacgcc atgcacgcga
     8161 cgaaggcggc cgtcgaggac ggcatcgtcc cgggcggcgg cgtcgcgctc ctgcgcgcga
     8221 tcgcggaggt cgacaagctc aaggagcacg gcgacatcca gatcggcatc aacatcgtca
     8281 agcgcgcgct cgaggagccc gctcgtcaga tcatcgcgaa cgccgggctc gagggctcgg
     8341 tgatcgtgaa ggagctgaag gagaagggcg gcttcatcgg cttcaacgcc gcgaccgaga
     8401 agacggagga catgctcaag gcgggcatcg tcgacccggc gaaggtcacg aagtcggcgc
     8461 tccagaacgc ggcgtcgatc gccggcctca tgctcacgac cgaagccatg gtctccgaga
     8521 tcaaggagaa ggaaaaggct ccggccggcg gcggcatggg cggcggcggc ggcatgggcg
     8581 ggatgtactg acctctctgg tcccgatcgt ttcctggaag gcccggtcga gagaccgggc
     8641 ctttcctttt ttttccctct ctgcgcctcg agggcctcgc atcgggagac ggtggaggaa
     8701 gattttcttt gaaggtgaat aacccgtgcg cggcgtcacg ctggagccgc caacccgccg
     8761 gcgaggcgcc ccgtcgaagc gcgtgaagga ggcgagaacc ccatgtactt tctaagagaa
     8821 tcttcctctt cctcttctct ttcccttctc tctctcgtct ttctcgcggc ggcggggacg
     8881 gccgccgccc agagcaacca gacgccgaac ctcgacaggc gcgaggcgca gcagcagcag
     8941 cgcatcgcgc aggggatcca gtcgggccag ctcacgccgg ccgaggcgtc ccggctcgaa
     9001 cagcgcgagg cgaagctgaa cgccgacgag gctcgcgcga agtccgacgg cgtcgtcacg
     9061 aagaaggagc gcgcgcacct ccagaacgag gcgaaccgag acagcaacgc gatcacccgc
     9121 aagaaacaca acgcgcgcgt cgcgccgcac tgagctcgag cggggggccg gccgtcgaac
     9181 cgccccccgc cgccggttag aatcgccgcg ttgagcgagc ggaacgccgt cgccgccccg
     9241 ctccgcctcg gcctcgcggc cggggtggtc ttcgcgcccc ttgccgcggt gctcgccctc
     9301 ggcgccggag ccgcctcgct ctggctggac gagatcacgt acgcccgtct gcagtccgac
     9361 tttgccctgc gggccgcgga gatcgggcgg tccgggtcgg cggtggcgcc ctacttctcg
     9421 aatttcttct attgcgacgt ccagaaggtg tttcagtccc ttctcgccgc gacggggctg
     9481 gtccggttcg agtcgagccc cgagtggctc gtcaggtccc tctccgtagc cgcgtatgcg
     9541 gcgaccgtcg tcctcgtcgg cgtccgggcg cgtcgccggg agcttcccgg cagcgggact
     9601 ctgggtgcgt tcctgtttgc cgccgccccc gtgttcctct actacgcgtt cgaagcccgc
     9661 gtctatgcgc tcgtctcgtt tctcgcggtg ctcctcctgg agcggatcga gaagacggcg
     9721 gcgcgggtca cggttcccgc gatcctggcc gttgcgttcc tcggcgtcgt ggtcgcgcgg
     9781 ctccacctgt ggacgctgtg cctcttcgcg gcgctcttcg cgagggcggc ggtcgaggtc
     9841 gttcgccgcc gccgcgcgac gccgctcgcc cgcgtgctgc tggcggcgag cgtgccggcg
     9901 ctcgcgacga ttttcctcga gtacggtttc atgaaggcga cgcagccgcc cgagccgctg
     9961 ttcgcgctct tcgcccgaca gccgctcggc ggaacccttc tgcagaccgg gctgtccatc
    10021 ttcgaggggc ctctgcaggt ccagtccgct ttccaggaac cgctcacccg ggccttcgtc
    10081 gccggttgcc tcgtgctcct gggtgttctc gtgatcgggg cgctccgcga gcccgaggcg
    10141 gaaggagcgt tcggcgcgcg gagcgccgcc gccgtcgcgc tcttcgctct cgcgatctcg
    10201 gtcgcgctcg ccgtcggttt cgggtatttc gtccacggcc gctaccaggt gccgctcttc
    10261 gcggttctct tctgggcggt cgcacgctcc ctcacgaacc gccggagact tcttctcgcg
    10321 ctgctgtttg cggcggcgga ggcggtcctc ctcccgtcga cggcggcggc gatcagggcc
    10381 aagtcgaacg acgcggaaat cgccggcgtc atcgcgaagg gaggggaccg ctccacgacc
    10441 gccgtggtcg tgcagcacgg cgtgatctcg ggctaccccg cgccccacca cacgatcggg
    10501 ctcgacttct acctcaacga cgtccacccg ggcggagcac ggatcccgct cttcgagctt
    10561 ccggacctgc gagcggttca gggagaccac gggacctacc ggtacttcaa cggcggcgcg
    10621 ccgcttctct cccgcattct cgagatccgg cccgaggtct tccggacctg ggccgaccgc
    10681 gaggccccgg ccgacgtctg ggtcgtccag cccctgtggg acgtcgcgcg gtcgcgcgaa
    10741 cagatcgcgg cgtttctcga ggtgctcgtc ggcgagcggc gatacgcagt tgccggcgcc
    10801 ttcgtggcgg acggctatcc gcgggcccgc gtgatccacc tccgccgcgg tccgcaggct
    10861 ccgggcgccg cggactcagg gccgggtcac gcggcgaacg cgatggtggg cgagcagacc
    10921 cgccagtgac ggaaagcgtc cgccgtccag gttcggatgg cggcggagcg cgggctcgag
    10981 ctcctcgcgc cagcccgcgc ctccgaggaa cgggaaacgg ccgggcccgt agagaaacgg
    11041 ctcctctccc ggagtcgcgg gcgctgcgga agcggggccg cggcggaaac gcgatccgaa
    11101 ggggaggcgg ctcagcgtgc ccgtgaacag ggtttctccc gtctcgacga agaggtccgg
    11161 ccaccagggg caatcgacga aggcgtcgcc cgtgatccgg ccgtgcgtcc ggagctcggg
    11221 ctcgagggtg cgccgggaag tggagggatg gtcgaagagc tcgaggggct tctcgccggg
    11281 ctgcacgagg cgcagcgcct tccgcaggaa aacgccgtac gacgccggat gcgtgacgga
    11341 gacgacgagc tgacggcggg cgcgcgcggc ggcctcggcg aggtagcgtt tccagtcggg
    11401 caccgacggg agggcgttgt acgtgaggac gaggtcgaac gtctcgtccg gccacgccac
    11461 gccggcgcgc gcgtcgaggc ggtcctcgag cccgaccgtg cggtacacgc ctctcacgac
    11521 ctccgcgcct tcggcgtccg ggacgatcac ggtgacgcgg gttccgcggg ccgcggcggg
    11581 caggaggtgc aggccgggaa tcccggccat cccgtccacg gggccctcga gggccgtcgc
    11641 gggagtctcg tcggtgaacc acttcccgag gagctcgtac acggcccatc gctcgtacgc
    11701 ggtcccgagg cccacgtcgc ggcgggccgg cggcgcgagg ggaacgagtc cgccggcgct
    11761 cgtcacgcgc ctgcctccgc gagcgcggcc gccgtgaggc gcaggcccgt ttcgtaggac
    11821 gtcgggggct cccagccgag gagtccacgc atccggtcga tgcgataggc ccggtcgacg
    11881 gtcacggtct gcagtttttc gcgcgtgacg ggcggctctc cgccggggaa gcggacaccg
    11941 agcttctcga gagtctcgaa ggcgattccg gcggcgcgcg cgacgccgag gggaacgccg
    12001 agggcgggga gggagcggcc cgcgatgtcc gcggtgagcg tcgacagggc gcggatggtg
    12061 atcggctcgc gataggtgca gatgaactcg gccccgtcga ggcccgggcg gagcgccgtc
    12121 tcggcgatcg cattggcgag gtcttcggcg tagacgagct ggacgcggct cgacccgctc
    12181 ccgacgatcc ggtaacggcc ggccttcacg aggcggaaga gcttgtcgat catcccgttc
    12241 gtgtccccgg gcccgtacgt gatcgacggc cgtacgatcg cgtacggcag gcccgattcc
    12301 cggaccatcc gctcggtctc gaccttgctg cggccgtacg ggcccacggg ggcgaacggg
    12361 cgcgtctcgt cgatcggggg atccggcggc cagccgaaca cggcgatcga cgagacgtcg
    12421 acgaagcggc cgacgcggcc cttcgccgct gcgaggaagc gcgcggtgag cgcgacgttc
    12481 tgccggccgt actcctcggg ggggacaccg tggcggtggc ggaccgcggc gacgtggacg
    12541 agggcgtcga cgggctctcc tccggtctcg aagtcctcga aggtcgtcca cgtgatcccg
    12601 ggacggggcg ccgggggcgt ggcgtggcga tgcagcgcga tgacgggaac gccgcgtgcg
    12661 gcgagcgtgc gcgcgacgtg gccgccgatg aacccggtcg cgccggtcac ggcgaccgtc
    12721 acggccggtg tccccgcgcg atgcgcggcc gcaggacgag gcggtcgtag agccccggcg
    12781 cgaagcggtc ggcgaggtcg atggcgcgtt cgcgccatcc gatgacgcgg ctctcccgcg
    12841 agccttcgag gagggcgcag agcgcgacgg ccgtctcccc gggcgacgcg ccgcccgtcg
    12901 cgggcggttc ggccattccg tgccaggccg cgcgggaacg aaacggcgag cggatcaggc
    12961 cgggagccgc ggcgacgaac cggatcgact cgggcgtctc gagatcgagc acctcgagga
    13021 aacggagcgc ggccgccttc gaggcgccgt agtaggactc gaagggaacg gcgcggcggc
    13081 ccgcgatcga gaggacggcg agaaacgtgc cgggcgtccc gtcgcgcgtc cagcgcgccg
    13141 cggccgcccg cgccgcctcc gtgagcgccc agaagttgat ctcgaacacg gcgcgtccct
    13201 ggtcttccgg aatggagccg gcgagtccga aggctccgga tccggccgcg tagacgattg
    13261 cgtcgagcgg gccgtctccg gacgcttccg cgaggaggcg gggccagtcg gcggacgcga
    13321 ggtccagggg gagaaacgag tcgacgacgc cgggatccgg cggcgtgcgg ccggcgccgg
    13381 tcacgcgcgc gccgcggcgc cgcaactctc cggccaccgc gtttccgacg gcgccgtgag
    13441 gcgcgatcac gagtgcgtgg cggggcgtca cgtctccgcc tcgaggcccc gcgcgagccg
    13501 ctcccacgtt cgcgcgcgcg agaagcgcga agcgtcgatc tcgaggaggc ggtacgtctc
    13561 ggacgggtcg tacacggtgt cgagcgcgag gatacagagg atctctcccc aggtccgcag
    13621 gtcgagtcgc ggcgacgagg agcgcgggag ccggcgcagg acctctcccg cggcgcccgc
    13681 ggggaagggc agggagaacc ggggacgatg gagctctcgg ccgatcgcgg cgacctcgcc
    13741 gctcgtgacg aagaaggggt cggcgacggt gagcggcggc tcgatcgcct ctccgcgccg
    13801 cgcgacggtg cggacggctt cgacgaggag cggcagcgcg cagaggtggt ggcgatgccg
    13861 cgcgggcgcg agccggagca ggagaccgtg gccggcgatc gcgccgaggg tcccgagcat
    13921 cccgtcgggc tcgcggccgt agaggatcgc gggcctgagg atcgcgaggc acacgccgcg
    13981 tgcggcggcg gcggcgcgcg ccgcgcgctc ggcgtcgcgc tcggcgacgg cgtagtccgg
    14041 gcagccgaac gtctcgggcg accccgcttc gtcgttcctc ggcgaggcgg ccgggctccg
    14101 gccgaggacg gacgcgctcg acacctggac gagaagcgct cccggcgaga ggcgcgcgac
    14161 gagccgttga acggcctccg cgtgcgtccg cctgtagtcc gcggggccga ggcccgggcg
    14221 gacgcgcacg ccggaagcgt tcacgacgac ccgggccgcg gcgagaaccg cccccgcgtc
    14281 ttcaacgggg atctcggcgg aagtgcgggg ggcgcggaaa acgacgggcg cggctccgag
    14341 ggccgaggcg agagcgcccc cgacgaatcc gtccgctccc acgaccacgg tcgtcatgcc
    14401 ggcgtctccg gcgcgcggcg gggcagcatt tcggcgaccg ccgggagcga cgcagcgccc
    14461 gcgacgaaca cgatcaggag ccacgctgcg tgatagagga gcgcggcggc cgccgtggcg
    14521 tccacgggct ggccgagcgc cgtggcgaag atgagcgtcg cgtaccactc ggtggtcccg
    14581 accccggcgg gcggcgccgg gacgaccgcg aggaggtcga ggagggccgt tccggccacg
    14641 gccacggcga aggggacggg atggccggcc gcgcggaaga gcagcgcgag gcccgccgcc
    14701 tgcgcgagga gcgacccggc ggagagcgcc gcaagggccg ccgcgaggcc gggccgcatt
    14761 ccgacctcca cggatgagtc cgcgaaagcg cgcagcgtcc gggcggtggc gtcacgcgcg
    14821 ggcgccggaa cgaagcggag gagcggcgcg ccggtccggg cgagcgtctc gccgtggcgt
    14881 ccccagacga agagcgccgc aaccgcgaga acgagcaggg ccgcgaagat cccgatcgac
    14941 acgcgcaggc tcgcggggag ccgcgagggg atgacgagta ggagcggcag cgcgaggagc
    15001 cgcgtggcga ttccccagag gcggtcgacg accatcgacg cggcggccga gccgaccgtg
    15061 aatcccgggc tgcgccccgc gagggcgacg gcgcgtccga ggtccccgac cttgaccggc
    15121 aggacgttgt tcagaagctc gctcgcgtag agcaagtgaa acgtccgcgg gagcggcacg
    15181 cggccgagcc ggttgaggag cgcgtgccag cgcagcagtc tcaggaactg ggcggcgccc
    15241 atgagcgcga aaccggcgag gacgaggccc gggctcgcgc gcagcgccag ggacgcgacc
    15301 cgggggccgt cgaacaggcg ccaccagaga gcgaacgcgg ccaccccggc gaggagggcg
    15361 aggccccacg tccgcgcgcg cgccctcacg acggaggcgc gccggagccc gccttcgtgc
    15421 tcgccgcgat cgaccagacg gtgtagttct tttcaccgcc gcggtaatcg cgccaggcct
    15481 cgagccgggc cagggcctcc gtgccggcga gcgcgagccc gggcagccag cgggagggat
    15541 ggggaagcac ttcggcgagc cagatcggga ggacggacgc tccggacccc gtggagaccc
    15601 tgtagcccgt gcggtgcttc agccagaggt ggccgaacgc gatacggcgg cgctgtgaca
    15661 tccactctcg atacgtcgcg ggaccgcggt tcccgatgac cgcgtcgggc acgtaggcga
    15721 gccggcctcc gcgctcccgc acggcggcct cgagagagga ctcgtcgacg gggctcgcgg
    15781 cgtcgagcga ctcgacgagc gcggagcgaa cggcgacgag ctcgccgagc ttcgggctgc
    15841 gccgcgcgac gcagtcgtga aggtgccaca tgagccgcgc catccggtcg aggagcgccc
    15901 gtccctcgtt ccgcggaacc ggcctcccgc ccgccattcc gacggcaggg tcggcgaagg
    15961 ccgcgacgat ccgttccgtc gcacccggct ccgggaggac gtcggcgctc gcgatgatcg
    16021 tcacgtccgt tccgggcggg cgcgccgcga ggaacgagtt gatcgcggcg gccttgccgc
    16081 ggcgctcggc ctcggcgatc agggcgacgc gcgcgtctcg ggccgcgaag tcgcgcacga
    16141 tctcgtcggt tcggtcgcgg cacgcgctcg agacgaccgt gaccgcttcg atcggcgcgt
    16201 cgaacgggcg ggccgcgagc agcgcctcga gcagagggcc gatcgtctgc tcctcgttgc
    16261 aggccgtgac gccggcgacg acgcgcatcg tcggcagtct acgccggcgc cgtcgccgcg
    16321 gccgagcccg actcggctcg aaggaccgcg aggagcgcga ggagatagag gacgccggcg
    16381 aggccgatca gggcccggta cccgagcagg agcgacgagt actcgagcat tcccccgagc
    16441 atcccgccga ggagattcag ggcgtagctc ttggctccct cgcgcgcgtt gcggaagaac
    16501 gtcgcaaaga ggacgttcgc gcagaagatc ggaaggaacg ccacggagct caccacgagc
    16561 gcccgccaga agtagtcccg cgtgaggagg gcgtcgagcg gcacgaccca cgagacgagc
    16621 agggacgcgc tgagcgcgcc gaagagtccg agcctcggga tgcgggggaa gcgggccgcg
    16681 acccagatcg cgagaaggac gctcacgtgg atggcggcga agacgaccgc gttcgtggcc
    16741 caggtcgagc cgaagaacaa cccgaaggtc gcgacgcttc gggtctcgag gagcaggaac
    16801 gcggcgccca tgaagaagaa cgccgcgaga tgacccggaa gctcccgctc gcccggcggg
    16861 gacggccccg cgagccgcag cccgaggacg acggagagaa gggagacgag cgcgacgatt
    16921 cccagcgaga cgagatagtg cggcggcacg ttcggctcga acagatagac gaacggccag
    16981 tcgtcccgcg cgagggtcgg ctcgtttggc gccggcggct tccgggacgc gaggtgcgga
    17041 agaccgggcc ccgccgccag gatcgccgga aagccccacc cgccgcgctc ggtctggacc
    17101 cggaagaccg cggggtcccg gtcgaacgct tcccgcagca ttccctcgat cttctgcacg
    17161 acccagggca gacggtagta gttgtagagc acgaagacgc cgtgctccgt gaggtgggcg
    17221 cggatctcgc gaaacgcctc cacggtgaac aggaagctct ccatccggag gttcgtgcgg
    17281 tcgctgaggt tgaacgtgga gtccggaagc ccgtagacga tcaggtcgta cttccgggtg
    17341 ctgtgccgga tgaaggcccg tccgtcggcg acgtgggtcg tcacgcgcgg cgacgaaaag
    17401 ggcttctccg ggtggaattg ccggccgatc tcgatgaccc acgggttgat gtcgaccgcg
    17461 tcgatctcgt cgacgccgtg acggatggcg tacgccgtct cgttcccggc gccgcatccg
    17521 atcacgagcg ccgagcggta gcggggaggg gcgtctcgaa acggagcgag gatctctccg
    17581 tagggcgagt cgtagaggtg gtggtagatc gcggattccg agggctcgtg gatcccgatc
    17641 ccgtagacgc cgttgcccgc cagcaggaac gtcttgtcgt cgatcttctt gaggttctgg
    17701 cggtagtagg gaccccaggt gtcgccgcgc cccatcatcg cgacgattcc gagcgcgccc
    17761 gcggccgcga gtccctggag ggcccacccg cgccgcggcg tcccggcgag cacgggaacg
    17821 gaagccagga ggcacacgcc gaaccagacg accgggggag cccagaggag ggagcagagg
    17881 gcgaaggccc cgattccgag gaggctgccg acgatgttga ccgtataggc gcgcaggcgc
    17941 gggagacggc cgaagaggac gcccatcggg gtcgcgagcg cggcgaagag cagggtcgtc
    18001 agggcgaaga ggatcgggac gagcaggtag gtcgggatgg ccgtccggtg gatgtccgag
    18061 gacggcgcct ccgcccagaa gacgacggag cgcgtcgcgg ggagctcgat gatccggacc
    18121 tggaacgcgt agacgatccc cgtcgtcgcg agaaggatcg ccgtgaagta ccggatgagc
    18181 gtctcggtcc tccgggccgc gaggcacccg agcccgatcc cgagaaacgc gcccagcagg
    18241 atgaagttcg tgaagtagcc gaaattgatg atgtacccgg agatccaccg gatcagggag
    18301 agctcgagga agagaaccgc gaaactgacc gtgccgaggt ccacgtcgtc ccggctcacg
    18361 aaggggggct ccggtttcag gtccgtcatg tgctcctact ttccctcggg cgccgcggga
    18421 agctcgatca tgaacaggtg agggaacccg aacatcatga acgaggagcc gcttctccgg
    18481 taggggctct cccgcacgat cttcagaaag tctgcgagct ggatctggct ctcctcgagc
    18541 ccccagatgg ggttgacgag gacgaggttt gcgggaagca aaggaagccg gctgcgccag
    18601 atctcgggcc gggacttcag ggtgcgggcg aggagggaag ggtcggacgc gaagaacggg
    18661 tagacgcccc tgcgcccgtt gacggagttc agctccggaa gctcgtacag ggaaacgggc
    18721 cgctcgttcg ggtgcaggaa gttcatgtag aagtccgccg cgatcgagtg cgacgggaac
    18781 gggtacccgc cggtcgggac gttctggaac acgaccccca ctcgtgagcg gtccgggaac
    18841 cgcctggcga cgtgggcggc gagtctccgg ccgtccgact tgcggtccat ttcctcgagg
    18901 ttgtcgggca ggagcagcag gttgacgagg acgaggcctg cgagcaggcc gcgcgggacg
    18961 gacccgcggg gcgggccgaa agcgaagagc agggccgcga gcagcgggag gtggtagcgg
    19021 gcgtgctgaa agaagccgaa cgtgaccgca aggaacgcgc agagggcgag cgcggccgcg
    19081 gcggtcagga tgtcccgtcg ccgctcccgg tcgccgtggc gccacgcgag aatcgcgatc
    19141 gcgacgagga ggaaggcgcc cgcccagacc aggatcttcc cggacggctg gttgaacacg
    19201 tagtactgca gatgcagcgg gcccgagaag ttcgagacga agagctcctc cagcgtcaga
    19261 cgcaggggct gggggcggaa cggcgggtac tgcggccggc ccgggtcggt gaacttcatg
    19321 aaggcgtatt cggcgacgat cagtccgagc gccggcaggg aggcggcaag ccgggcgagc
    19381 gcctgaggcg tcggtccgcg gctccgggcg aggcgcaggg ccccgtcggc cgccagggcg
    19441 gcgaagaggc agacggtcca gagatgcgag tgagccgtca ccaggccgag ggcggccagc
    19501 gcgaccgtgc gcccggggac caccccttcg tcggcgcgcg ggaggagcga gagcaggagg
    19561 acgacgagga atgcggtgaa cacgtagacg cgcgcctcga acgcgtagtg aaggacgaga
    19621 ggcatggacg agaacgcgag cgtcccgagg acggcgtcga ggcggcccga ttgcctgagg
    19681 aacgagcggt agaagatcgc gcacgcgccg agaaacgccg ccagcgggag caggcggagg
    19741 gtcgcctcgg ggtgcgactg aacgctcagc ccgagaggcc cgaggaggcc ctggaacagc
    19801 cgctgaaggt cgcagaagaa gaagttgccg aagtacggag cgagcgagga ggccgggcgg
    19861 ccgatctcgg ccgcccggag cgcgaaatcg tcctggagat agaagtagag gatctcgtcg
    19921 agccagagcg gcgctcggaa tgcgccgaac gagatgcaga gaacggccgg gaccagagcc
    19981 agcccgaatg cggcgacgtt cgccgcggcg cgccgcgggg gccggtcctc ccgcgtcacg
    20041 cgatccagtt gcgcgcgacg cgcacgagcg accgcggcga cacgagcatg ttcgtgtagc
    20101 tcgcgttcgc gagcgggcag gcgcattcgc ggttgtagac gctcttccgg aaccggccgg
    20161 cctcgggtga gaaccacacc ttgccgaaat cgtagccgtt ttcccgaagg cccccgaccg
    20221 actcggcccg gatgcagcag ccccagacgt agccgtcggg ggcgatctgg gcggaagccc
    20281 agccggcgta gcacgggatg atctgctcgc ggcgctcgag gtactccttc acgagctcgt
    20341 agtactcgag gcggaagctc tcgacgaggc ggcccatcgg atgccggagg cggttccggc
    20401 gcatttccgc gatgaggtag tcggcggcgg cggcgtattc cgcggccgac ggcgtgatgt
    20461 ccttgcccat cgtcccgagc tcgacgcgtt cctccgcgac ctcggtgatg taggaatcgg
    20521 ggttcagctt cttcagctcc gcgtggattt cggggatccg cgccacgttc tggctgctca
    20581 cgacggtgtg gatcccgacg gtgacgtgcg ggttcttcct ctggagctcg aggacgagcg
    20641 cgcgcgcctt catcatcttt tcccagttgt cccgcgcacc ccggatctcg tcgtgtcgcg
    20701 tcccgacctc gtcgagcgag aggttcacga tcagcttcga gcccttcgtg gcctcgacga
    20761 tgccgggcat ctgcttctcg atctgcttcg tgaggagccc gttcatcggg aggttgatga
    20821 tcttcggcct caggatccgg cggatgagcc cgaagagctc gggcaggtcg gagcggacga
    20881 actggtcgcc cccggagacg gtcacccaga tcggcacgcc ctccatcgag cggaacgtct
    20941 tctcgtattc ctcgagcgtg aagtcgggga ccttcttctg ccagatgtcg caggtcttgc
    21001 accgcgacgg gcaggtgtac gtcacgctca ccgtgaggtt gatcggcagg aggcgcgggg
    21061 agccgaaccg gtggaacgag cgaaacgcga ggagcttgag cagcagggga gcgcggtcga
    21121 tcgcgctcaa aggggcgaac cccactcgtg ccacgactcc cagccgcgtt tgatcggggt
    21181 cttcgggtcc gcgggccccc cgggaaggaa cagccggcac cagaacgggc gcgcgacccc
    21241 ctcgaggacg cgccccgacg agatcttcga cgtgcccttg cgccgctcga cgaagaggat
    21301 gttcgtctcg gcgagctggc ccgaggcccg ccagatccgg tacttcatct cgatctggta
    21361 cgcgtagccc gaggaccgga tgtcgcggta gccgatcttc tcgagcatgt cgacccgata
    21421 gcagcagaag ccgctcgtca ggtcgtcgca cggcacgccc gtgatgaggc gcgagtagac
    21481 gctcgcgaat gccgaaagga agacccgacg catgggccag ttgaggacgc tcacgccata
    21541 gaggtaccgc gatccgacga cgagatcggc gtcgtgggac atgtcgatga ggagcggcag
    21601 aaaatccgcg gcgtgcgagc cgtccgcgtc catcgacacg acgcggctga agccgtgctc
    21661 gatcgcccag gagattccgt ccttgtaaga gcccgtgaag ctccgcggcg gttttctgcg
    21721 gagcaggtga aggcgcgggt ggcgcgcgcg cagcgcctcg acggcgtccg cagttccgtc
    21781 cggcgactcg tcgtcgacga tgaggagcgt cacctcgggc agcacgtgga acacggccgc
    21841 gacgtggtcg gcgatcgtct cggcctcgcg gaacgtcggc gaaacgacga gcgtttccct
    21901 cgacagcgtc ccgacgcggt cgaggcgctc ctgcgagaac ggcgcggaca gatccacggt
    21961 ggaatgatag gcgaggggcg cgggacgccc gggcggggcg ctcgcggacc cataatcgcc
    22021 ggtcgatgct cccggggacc ctt
//
LOCUS       DAOMFY010000034        21869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_765_length_21869_cov_8.348354, whole genome shotgun sequence.
ACCESSION   DAOMFY010000034 DAOMFY010000000
VERSION     DAOMFY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21869
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_765_length_21869_cov_8.348354"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..504
                     /gene="mce"
                     /locus_tag="PLB01_04835"
     CDS             109..504
                     /gene="mce"
                     /locus_tag="PLB01_04835"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQQ76658.1"
                     /translation="MSHLGISVPSLEQGGTFWDLLGLVEEHREEVASQKVLTSFRAVG
                     ESHLELLEPTSPDSPIAKALEKRGPGIHHLCLEVTDVRAALAKLKAAGVKLVNEEPFD
                     GAHGCLVAFVHPSATGGILLELSEKKKRP"
     gene            complement(501..1208)
                     /locus_tag="PLB01_04840"
     CDS             complement(501..1208)
                     /locus_tag="PLB01_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76659.1"
                     /translation="MQGILPRERHGTAWLDGPRARIEIVTEAGRVAALRNPAAKDACT
                     NLDLGRRTFYPVPCEMALANAEGLMPQFANPQAHARGGATEAPRRSRPRLEESESSGE
                     EIAGFPTRRLAWTASWSESQRIGPDTVRIEISKTLEAWTTDRVTQEAFRFGHADELAA
                     APEEVRAHIAERLAAAGFPLKLVVRSTRKFDRGEPTTETLTTVLEDVQAAAPEPSLFE
                     VPEGYRHEKPLVGIPTR"
     gene            complement(1295..1630)
                     /locus_tag="PLB01_04845"
     CDS             complement(1295..1630)
                     /locus_tag="PLB01_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76660.1"
                     /translation="MEAGTLVLVHLVAPKEKYWGLLEALSPAGLTLRGLELDLFEEWA
                     RQQRPGADAELGVTTLFVPLHRVEKIFEDTRVGSVASYTERFFVIVGRDVREVLRPAP
                     PVADTGNPN"
     gene            complement(1640..2437)
                     /locus_tag="PLB01_04850"
     CDS             complement(1640..2437)
                     /locus_tag="PLB01_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76661.1"
                     /translation="MPRHSVARAAAFLLALALGWAGTATATYVIFTKDGKRIEAQEKP
                     VLKGKRLTFLTPLGSPQSIAVEEFDQERSDKVNREGLGNAYLLDDPTGQRSSLPNPEA
                     KKPSLSEYIRQHGKKSMDSSQPDAKQGELVKQRPVESEMRAAARAAIAAEAPTIVMDP
                     QITEAFMRAMDGSNVKGVKLTQIPSGVRVTATTETEQQVFLALVACARGLKESRAAGK
                     PLDKAEVAFTTSTGETAARFDLSPNDADNLLTGRVTPSRFFVSNVIF"
     gene            2527..3528
                     /gene="aroF"
                     /locus_tag="PLB01_04855"
     CDS             2527..3528
                     /gene="aroF"
                     /locus_tag="PLB01_04855"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQQ76662.1"
                     /translation="MDRTATGIDVGRVVAAAEKLGLKAHPIPGAQRTAIGITGNKGTV
                     GPAAFENLPGVLEVIRVSHPYKLVSREFHPEDTVVSIGGVPVGGTRLVVMAGPCAVES
                     LEQTVTIAKAVKELGAHLLRGGAYKPRTSPYSFQGLGEEGLKILAAAREETGLPVVTE
                     VLDTSTVDLVARYADCLQIGARNMQNFELLKLAGRAGKPVLLKRGMSATLEEFLLAAE
                     YVLAEGNPNVVLCERGVRTFNDFTRNTLDLAVVPAVERLSHLPILVDPSHGTGRRDKV
                     LPMSLASVAAGADGVAIEVHHRPAEALSDGPQALTPELFGNLMAKLRPVATAVGRVL"
     gene            3525..4631
                     /gene="carA"
                     /locus_tag="PLB01_04860"
     CDS             3525..4631
                     /gene="carA"
                     /locus_tag="PLB01_04860"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQQ76663.1"
                     /translation="MSGRAVLLLEDGRKWEGRSVGPAGTSFGEVVFNTGMAGYQETLT
                     DPSYRGQIVVMTASHIGNYGLNDEDVESDRIQVAGFAARNFPDRFSNARGRETVAEAL
                     AKAGVPGIDGLDTRSLVMHLRSAGAMRGAISSEVASDADAAALLEKVKAQPAMAGAAL
                     ALTVSTKKAYDFPMPPGAAVKGRIAAVDYGIKTNILRMLAARGLKPTVFPATTPAREI
                     LAQGFDGVFLSNGPGDPDALPGPVANVREFVASGRPVFGICLGQQLLGLAVGGTTYKL
                     KFGHRGANHPVKNLRTGHVAITSQNHGFSVAADSLPKYAELTHVNLNDGTCEGFRLTD
                     KPVFAVQYHPESAPGPHDSDALFDEFVSLVARPR"
     gene            4697..5554
                     /locus_tag="PLB01_04865"
     CDS             4697..5554
                     /locus_tag="PLB01_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HQQ76664.1"
                     /translation="MSARARTSLAFLAALGLLVTPAARAADGKAEGTITVNGKTTKLA
                     HAYARAVKGFFDKTKEDVEVILSDVPLEAKALEDPFERSRMADAGKLHAFEITIDADG
                     KPISTAFRDNGFKKASPSGLSSADVFTKKVFDGKTVEGSYKSAKESEFFGNTYSFDVS
                     FKANVARAPKPVQPTAAETAAAQKSPQAAVYADFLRAIQKEDLPALKKLFTKEQAKNL
                     DDPEAKKMVGMVKMMSPTEIKVLRVVETGDKADLTVTGKQDGSVASGVVHMVKEGGAW
                     KVQREEWKN"
     gene            5571..6803
                     /locus_tag="PLB01_04870"
     CDS             5571..6803
                     /locus_tag="PLB01_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ76665.1"
                     /translation="MHVPAFLAKRFLAGSRRGLPKTVSVLALCGVALGAASLVVAMGL
                     MSGYRHDLAERIAGTSAEIIVMPDAATVPGEARKALEALPNVSAVARTGFAPALLLSA
                     AAPQGADALVKALDLPAGLATTRLLGRVPGVEAAFASLRAKGETPVLLGAGLARRLGA
                     AAGSRVVLETASSALSAGVAPPTRTALTVAAVVETGFSEVDDSWSVLALRDFDRIAPP
                     DARMGVLEVKLERSSGSTETVASARALLGPGATVLDWKVLNRDLFQALAVQQTLLFVV
                     LTLIVAVAAGTVVSSLVVLLAEKTRDVGVLAALGAPPRVIARTFRLSGLILGGGGLVL
                     GVLFGLLVCVFLTTFHVVRFPPEIAKVYYLAWMPFRPEPLHVLAILGVGLLLVLAASA
                     LPARRAARLNPSDALRYE"
     gene            complement(6855..>8051)
                     /locus_tag="PLB01_04875"
     CDS             complement(6855..>8051)
                     /locus_tag="PLB01_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76666.1"
                     /translation="GAEDNTTRAFRDGKEAVFLGRTTEQPETDPNDYLYSVDLGSGAV
                     RRVSTSLPFSRSTLLNQVVDVGADDRSIVLSVPAGDLTAIVEVPRGGGAPRALTTVTQ
                     MVWGLGVAKNGDIYVDQVLRPQDALLMEPDGGRPQTVLSVNNLVYTPTLPLPDGRILF
                     TVSVDRRSRLLAARPRGNPTPFAQTAEETSGPLAMVGSSEVAFLAGPPAARKVAIASV
                     ADGRIVRRLEEADGPSIEALASSPDGRTLYYAARGKIWAIPAAGGPPKPVCEGDSVAA
                     DPRGEHLVVQLNEKGTVRLMRVPVGGGPEQPLSFPGVRPAAVLMTSNAVRADGAILKT
                     SNASDTWPWYVAVLNPESGAAQRITLPSFLDAQYPGWTADGKILAFGARTHATVWRLR
                     RESKPR"
     assembly_gap    8052..8065
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8066..9513)
                     /locus_tag="PLB01_04880"
     CDS             complement(<8066..9513)
                     /locus_tag="PLB01_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76667.1"
                     /translation="MGEVYRARDPRLARDVAIKVLPEEFFEGEERRQRFEREARLLAA
                     LNHPGIAAIYSFEEIPSFSSPSSRHVLVMELVEGDDLAVRLSSGPLPLDESLSLARQI
                     AEALEAAHDKGIVHRDLKPANVKVTPEGRVKLLDFGLAKVFEGDAGPGSAPSVTHSPT
                     LTARATAAGVILGTAAYMSPEQARGKPLDKRTDVWAFGCVLFEMLTGKRAFDGETVTD
                     VLAAVLTKEPDWAALPAQTPGAVKKILRRCLQRDARLRLHDIADARLDLDELSAAASS
                     SSSAQLPFEENLHPASPSPTVASAERASRERGSRKSLSFLLPWALAAAFAAALGAMWL
                     SSRSRPDAPWSGVMLGGPEIAMVPRPSPDGRLVAFQAMVDGQTQVAVMQPDSGDWTVL
                     TRDRTRGVATSMSWSPDGTRIYYGRQDGVPRGVFSVPAVGGEERLVLEDARSPEVLAD
                     GSLLVHRLNEKRQPQLYRFWPDGGRLQAYTALP"
     gene            9658..10095
                     /locus_tag="PLB01_04885"
                     /pseudo
     CDS             9658..10095
                     /locus_tag="PLB01_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
     gene            10342..11109
                     /gene="deoC"
                     /locus_tag="PLB01_04890"
     CDS             10342..11109
                     /gene="deoC"
                     /locus_tag="PLB01_04890"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQQ76668.1"
                     /translation="MIACVGPGCRVSGAPGGGEVPRDLAGYIDHTLLRADATYADIDK
                     LCDEAAQYGFASVCVNPVFVKRCADRLAGMSPAVCSVVGFPLGAASREIKALEARRAI
                     RDGASEIDMVVAIGALKSGDLRTVENDIRAVAETAHDGGAILKVILETSLLTDDEKVA
                     GCVAARKARADFVKTSTGFSTGGATAHDVELMARAVDRKLGVKASGGIRSAEDAEKLI
                     AAGATRIGASVGVKIVQEARGIAPASKGDAKAAKGTY"
     gene            11106..11744
                     /locus_tag="PLB01_04895"
     CDS             11106..11744
                     /locus_tag="PLB01_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019494149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76669.1"
                     /translation="MMADLRSFVYLDSMQPQVAAYIGTVARGFLPVAREASLIVEISP
                     GIEINRVTDVAVKATDVRPGMQVVERLYGMLEVHSISQAEVRAAGAAILAALSLNEAD
                     RIKPRVLSSQVIRRVDALQAQLINRDRQGMMLVAGESLYILEVEPAAYAAFAANEAEK
                     AAEINVVDVRMTGAFGRVYLGGEQRDIDVASVAAIRAIESLTGREAQKGRRD"
     gene            11749..12372
                     /locus_tag="PLB01_04900"
     CDS             11749..12372
                     /locus_tag="PLB01_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76670.1"
                     /translation="MIELRSYVFLDSLQEQHAAFLGTVARGFLPVGGQASLFIEVSPG
                     IAVNRLTDVAVKATSVTPGMQIVERLYGLLEVHHDEQAEVRRAGEAILREIGKSEDDR
                     IRPEILSSTVIRGLDDRQTQLINRTRHGNMIVAGQSLYILECQPAAYAALAANEAEKA
                     AHIKVLEIVSFGSFGRVYLAGLDRDIDVAQPAAERAVRGLSGRAAKS"
     gene            12430..12747
                     /locus_tag="PLB01_04905"
     CDS             12430..12747
                     /locus_tag="PLB01_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HQQ76671.1"
                     /translation="MAEALGMIECRSFAAMVEAADAMVKAAKVELVGYEKTGGGYVTA
                     VIRGDVAAVKAAVDAGQNGASRVGDVVTTHIIARPHVNVDLVLPLGRAAEAGKETGAH
                     VKK"
     gene            12776..13093
                     /locus_tag="PLB01_04910"
     CDS             12776..13093
                     /locus_tag="PLB01_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HQQ76672.1"
                     /translation="MLLAKVVGTVVASRKDPKIEGLKFLLLRQVNPENLREGGFVVAN
                     DAVGCGVGEYVLYASGSSARQTEVTKDRPCDAVIMAIVDTWEIGGQTLFEKNSSEHGV
                     RGA"
     gene            13113..14540
                     /locus_tag="PLB01_04915"
     CDS             13113..14540
                     /locus_tag="PLB01_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HQQ76673.1"
                     /translation="MSSVRDPGQIRRIAEIIAREIVAQTDAGPSPPSAGGGSFPSVDG
                     AVAAAAVAFRALDAVGLEKRRTIVESMREAMRRHARSLAAMAVEETGLGRAEDKLQKN
                     LLVTDKTPGPEELTPSATSGDHGLVLVEPAPFGVIGAITPVTNPSSTIICNSIGMVAA
                     GNTVVFNPHPSAAKVSRETVRLLGEAIVKAGGPPNTVTCTADPTIQSAGELMKHPRVR
                     LLVVTGGGAVVAAAMNSGKRAICAGPGNPPAVVDETADVGKAGRDIVFGHSFDNNVIC
                     TDEKEVIAVDAIADALKAAMTKAGAFELDARDLPRLENVIFEKGAGPRGHAVVNKKFI
                     GKDASVILRELGIAAGPEVRTILVEVPNEHPLVWTEQMMPVLPLTRVKNVGEAIDLAV
                     AAEGANFHTATMHSHDLAALSTMARRCNCSIFVKNGPNLAGLGYGGEGHTSFTISSPT
                     GEGLTSPRSFSRWRRCTLVDHFRIV"
     gene            14548..15153
                     /locus_tag="PLB01_04920"
     CDS             14548..15153
                     /locus_tag="PLB01_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HQQ76674.1"
                     /translation="MNDETPGPALAMVLLDDVPLGLAALDVLAKEADVEVLQTGTISG
                     GRYLILFGGEVGPVERSWRRALDTAGPRVVDEVFLPHAELRLAPAIGTGAIRWPAPGD
                     TLGVLQAATPPALVRAVDAALKGALVDLVELRLGDGLGGKAIASLWGETHDVEAAIAL
                     ARSSFERVDAKGASTAVIPQAARAVREALASPSRFFGEFLG"
     gene            15170..15457
                     /locus_tag="PLB01_04925"
     CDS             15170..15457
                     /locus_tag="PLB01_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HQQ76675.1"
                     /translation="MKLGRVIGTLVATVKPESMKGLKLLIVRPLTADLADDGDPFVAT
                     DASAQASSGDLVTWEASREAALLHDPWFIPVDHAVVGIVDQHSLDDPGRSR"
     gene            15528..15815
                     /locus_tag="PLB01_04930"
     CDS             15528..15815
                     /locus_tag="PLB01_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HQQ76676.1"
                     /translation="MILGRVVGTVVSTVQHPFYAGKKQLLVGAILPDGTLDGTRYVLA
                     LDMVGAGVGQTVLVEDEGNSARQILADPKAPVRSLIVAIVDSVDVPASGAG"
     gene            15967..16680
                     /locus_tag="PLB01_04935"
     CDS             15967..16680
                     /locus_tag="PLB01_04935"
                     /inference="COORDINATES: protein motif:HMM:NF036524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQQ76677.1"
                     /translation="MNRARTPRGEETRDRIRAAALALFQEKGFEASTMREVASLADVA
                     TGAAYYYFPSKEALVLEFYTRVQDELETTLPAALEGRRDLGERLKAVLELKFSLIRPY
                     RRVLGALVRAGLDPENPVSPFSTDTAPLRGRHLAIFERALEGSEVKIPPDLARHLPRL
                     LWLYDMGLVLFWLFDRSEGQARTDRLIARSLETIVTAIHLARFRVTALLRKAVVRLLA
                     DIEESPHDPHLAGAFAQRS"
     gene            complement(16668..18086)
                     /gene="glnA"
                     /locus_tag="PLB01_04940"
     CDS             complement(16668..18086)
                     /gene="glnA"
                     /locus_tag="PLB01_04940"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HQQ76678.1"
                     /translation="MFQQFDEAAAHVRQHAVTMVDLKFCDLAGRWRHVTLPAERFTPQ
                     LMEDGVGFDGSSVGLRSVKSGDLVLVPDLATAFLDPFPEEKTLSFLCRILEADDRSPV
                     LFDPRTIARRAEERLRASGVGTRSLWGPEFEFYLFDGVAYENGVNVASYRVESSEADW
                     RSHELGSGFTIPRHGGYHALPPQDHLDDARTKISRLLESVGVPVKYHHHEVGGPGQCE
                     IEIPLRGVLDACDAVLMVKYVVRRAAKKMGLTATFMPKPLYGEAGSGMHCHQLVLDGE
                     RNAYFDPKGYGCVSETGRFAVGGLLQHGAAVMAFTNPSTNSYRRLVPGFEAPVTAIYS
                     LGNRSAAIRIPKYANRPESARFEFRPPDATCNPYLAMAAQLLAGLDGVEKKMDPTALG
                     FGPFDGDVAALPPEQRAKIRALPTALHESLDALESDHEFLVKDGVFPADLVPRWIQKK
                     REEDREVRNRPHPYEIELYYDL"
     gene            18176..19315
                     /locus_tag="PLB01_04945"
     CDS             18176..19315
                     /locus_tag="PLB01_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ76679.1"
                     /translation="MNEIPDVYRELREHIDVMPVGFPATESGVELSILRRLFTPEEAE
                     AALLLSALPEPLARIHRRAPRGRWTAAELETLLDRLASKGAIMGGGGAVPGRKKKWSK
                     AMLALGMYEFQVDRLTPEMQRDMERYIAEGFAGAFLGPKTKQMRTIPVNARIVVERAV
                     GRYDDARDLIAGSDGPWAVLNCVCRQGRDLTGEPCRQTDVRKTCLALKGVARHLLETG
                     SGEELSRAQALALLERAERDGMVVQPENAQNPLFICFCCGCCCGVLRMAKQFPRPADV
                     VHSNFRAEVGAEACIDCESCRPRCPMDALDTVGGKTAVDHARCIGCGACIGVCPSEAL
                     RLVEKERPAAPPETHTALYKRILVERFGILGTARMIGSVILGRRV"
     gene            complement(19349..19987)
                     /locus_tag="PLB01_04950"
     CDS             complement(19349..19987)
                     /locus_tag="PLB01_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76680.1"
                     /translation="MTAPKVIPSEARWPVLVALLALGLLNFALPPTLILGPRWIVLAV
                     LAVLTVPTMLAHRAGRASLTKALGFAMSGVVTLALVGSLALLITGLPKKAETPAELAQ
                     SAACLWIANVLVFAIWYWRLDAGGPYSRDGRGVHTEGDFFFPQMMDGCPGPRNWSPRF
                     VDYLFIAFNNSTAFSPTDTPVLSRWAKVLTMLQSAISLLVVAVLASRAVGLI"
     gene            complement(20031..>21869)
                     /locus_tag="PLB01_04955"
     CDS             complement(20031..>21869)
                     /locus_tag="PLB01_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76681.1"
                     /translation="FQSAGDLAFALESLTSETAARAAVAPGVAPSRLVKRRALLAAAA
                     ATLLAAGFLAGRMGRKSAPAPTLLFERLSSDPGIERSPALSPDGETVAYVKAVSSHRH
                     VFVQRVGSDKAIDLSADSPEDDRFDPVFSPDGSLIAFRSGKDGGGIFVMGPLGESVRR
                     VTDTGYGPDFTPDGKEIVYAEEAALSPLARGTRAKIWAVEVATGKKRVLYGPDAVEPA
                     VSPHGKRVAFWGLVGDTAQRDVWTVPLAGLKDGEKPVPVTNDAAVDFSPFWSADGSFL
                     YFGSDRGGSFNLWRIAIDEASGVPRGAPESVTVPVTWAGTFPGSFRGSRNGKRVAFTS
                     PAELMTIEKLALDPRSLEPQGPPVVLRRSSIAFQDLTISPDGATLATRTVGRLEDLCL
                     VSSDGQKLRRLTHDEFRNRAPSFTANGARVVFYSTRDGDYGLYSIAADGSGLARVTPP
                     KSRFYLYPALSPDGRFVAAASFDTKVAVFPLTAKDGVPTAGPPVAEFDSRWPWSWSPD
                     STRLLAAGPGGDLALEAHLCTPGKGPCESLGVRTYVGVFAPDGRRVVLSTPDGVRVLD
                     LATKQSRLILPAAQGTLQRITLSPDGRALYFLRDVTESDIWVGTFR"
BASE COUNT         3289 a         7555 c         7765 g         3246 t
ORIGIN      
        1 ctggaggggg gcgccgtcgt cgccgcaggc tcctccggcg cccctgtcag tccttccccc
       61 tcttcacctt ctaagaagtc tccttcttcc tcttctctcg tctcccgtat ttctcatctc
      121 ggcatctccg tcccgtccct cgagcaggga gggacgttct gggacctcct cggactcgtc
      181 gaggaacacc gcgaggaggt cgcgtcgcag aaggtcctga cgtcgttccg cgcggtgggg
      241 gagtcgcacc tcgagctcct cgagccgacg tcgcccgact cgccgatcgc gaaggcgctc
      301 gaaaagcgcg ggcctgggat ccaccacctc tgcctcgagg tcacggacgt ccgcgccgcg
      361 ctcgcgaagc tgaaggcggc gggcgtgaag ctcgtgaacg aggagccctt cgacggcgcc
      421 cacggctgcc tcgtcgcgtt cgtccacccg tccgcgacgg gcggcatcct tctcgagttg
      481 tcggagaaga agaagcgccc ctagcgcgtg gggatcccga cgaggggttt ctcgtgccgg
      541 tacccttccg ggacctcgaa gagcgacggc tcgggcgccg ctgcctggac gtcctcgagc
      601 acggtcgtga gcgtctcggt cgtcggctcg ccccggtcga acttccgggt cgagcgcacg
      661 acgagcttca gcgggaaccc ggcggcggcg aggcgctccg cgatgtgcgc gcggacctcc
      721 tcgggcgcgg cggcgagctc gtccgcgtga ccgaaccgga acgcttcctg cgtgacacgg
      781 tccgtggtcc aggcctcgag cgtcttcgag atctcgatcc gcaccgtgtc cgggccgatc
      841 ctctggcttt cgctccacga agccgtccat gcgaggcggc gcgtcgggaa gcccgcgatc
      901 tcttctcccg agctctccga ctcctcgagc cggggccggc tgcgccgtgg cgcctcggtg
      961 gcgccgccgc gggcgtgcgc ctgggggttc gcgaattgcg gcatcaggcc ctcggcgttc
     1021 gcgagcgcca tctcgcaggg aaccgggtag aacgtcctgc ggccgaggtc gaggttcgtg
     1081 caagcatcct tcgcggccgg gttccgcagg gccgcgacgc ggccggcctc ggtgacgatc
     1141 tcgatgcgcg cgcgcgggcc gtcgagccag gcggttccat ggcgctcccg gggcaggatg
     1201 ccctgcacgg tcgtccggaa ccggtacgtg agcccgcccg cgtggagagc gcccgacgcc
     1261 aggagcagca gggcgagggc gcgggcgcgc gggctcagtt cgggtttccc gtgtccgcga
     1321 ccggcggcgc cggccgcagg acctcgcgca cgtcgcggcc cacgatcacg aagaacctct
     1381 cggtgtagct cgcgacgctc ccgacgcgcg tgtcctcgaa gatcttctcg acgcggtgga
     1441 gcgggacgaa gagcgtcgtg acgcccagct cggcgtcggc gcccggccgc tgctggcggg
     1501 cccactcctc gaagaggtcg agctcgaggc cgcggagcgt cagccccgcc ggcgagagcg
     1561 cctcgaggag gccccagtac ttctccttgg gcgcgacgag atggacgagg acgagcgtcc
     1621 cggcttccac gcgtccgctc tagaagatga cgttcgagac gaagaagcgg gacggcgtca
     1681 cgcggcccgt caggaggttg tccgcgtcgt tcgggctgag gtcgaagcgg gccgccgtct
     1741 cgccggtgga cgtcgtgaac gcgacctccg ccttgtcgag cggcttgccg gcggcgcgcg
     1801 attccttgag gccgcgcgcg caggcgacga gcgcgagaaa gacctgctgc tcggtctcgg
     1861 tcgtggccgt cacccgcacg ccggagggga tctgcgtgag cttgacgccc ttcacgttgc
     1921 tcccgtccat cgcgcgcatg aacgcctctg tgatctgcgg gtccatcacg atcgtcggcg
     1981 cttcggccgc aatggcggct cgcgcggccg cacgcatctc gctctctacg ggacgttgct
     2041 tcacgagctc gccctgcttc gcgtcgggct gggacgagtc catgctcttc ttgccgtgct
     2101 gcctgatgta ctcggagagc gacggcttct tcgcctcggg gttcggaagc gacgaccgct
     2161 gcccggtcgg gtcgtcgaga aggtaggcgt tgccgagccc ttcccggttc accttgtcgc
     2221 tgcgttcctg gtcgaactcc tcgaccgcga tggactgcgg gctcccgagc ggcgtcagga
     2281 aggtcagccg cttgcccttc aggacgggct tctcctgcgc ctcgatgcgc ttgccgtcct
     2341 tcgtgaagat gacgtaggtc gccgtggcgg ttccggccca gccgagggcc agcgccagga
     2401 ggaaagctgc agcgcgggcg acggaatgac ggggcatcgg gaacctccgg ggccgcgagg
     2461 gatcggggaa tcgcggcgac atgaagtata aacgcgggag agggaacccc agccgtgctc
     2521 atcgtcatgg accgcaccgc caccgggatc gacgtcggac gcgtggtggc cgcggccgag
     2581 aagctcgggc tgaaggcgca ccccatcccg ggagcgcagc gcaccgccat cggcatcacg
     2641 ggcaacaagg gcaccgtggg gccggcggcc ttcgagaacc tcccgggcgt cctcgaggtc
     2701 atccgggtct cgcaccccta caagctcgtc tcgcgcgagt tccaccccga ggacacggtc
     2761 gtctcgatcg gcggcgtgcc ggtcggcggg acgcgcctcg tcgtgatggc gggcccgtgc
     2821 gccgtcgaga gcctcgagca gaccgtcacg atcgcgaagg ccgtcaagga gctcggcgcg
     2881 cacctcctcc gcggcggcgc ctacaagccg cgcacgtccc cgtactcctt ccaggggctg
     2941 ggcgaggagg gtctcaagat cctcgccgcg gcgcgcgagg agacgggcct cccggtcgtc
     3001 acggaagtcc tcgacacgtc caccgtcgac ctcgtcgcgc gttacgcgga ctgcctccag
     3061 atcggcgcgc gcaacatgca gaacttcgag ctcctcaagc tcgcgggccg cgcgggcaag
     3121 cccgtcctcc tcaagcgcgg gatgtcggcg accctcgagg agttcctcct cgcggccgag
     3181 tacgttctcg ccgagggcaa cccgaacgtc gtcctgtgcg agcgcggcgt gaggacgttc
     3241 aacgacttca cgcgcaacac gctcgacctc gcggtcgtcc cggcggtcga gcgcctctcg
     3301 cacctcccga tcctcgtcga cccgtcccac ggcaccgggc gccgcgacaa ggtcctgccg
     3361 atgtcgctcg cgtccgtggc cgccggcgcc gacggcgtcg cgatcgaggt ccaccaccgg
     3421 ccggccgagg cgctctcgga cggcccgcag gccctcacgc cggagctctt cggaaatctg
     3481 atggcgaagc tgcggccggt cgcgacagcc gtcgggagag tgttgtgagc ggccgcgcgg
     3541 tcctcttgct cgaggacggc cggaagtggg aaggccgctc cgtcgggccg gccggaacgt
     3601 cgttcgggga ggtcgtcttc aacaccggga tggcgggcta ccaggagacg ctcacggacc
     3661 cgtcctaccg gggccagatc gtcgtcatga cggcgtcgca catcggcaac tacggcctga
     3721 acgacgagga cgtcgagtcg gaccgcatcc aggtggcggg tttcgcggcg cgcaacttcc
     3781 cggaccgctt ctcgaacgcg cgcggccggg agaccgtcgc cgaggctctc gcgaaggcgg
     3841 gcgtcccggg gatcgacggc ctcgacacgc gctcgctcgt catgcacctc cggagcgcgg
     3901 gcgcgatgcg cggcgcgatc tcctccgagg tcgcctccga cgcggacgcc gcggcgctgc
     3961 tcgagaaggt caaggcccag cccgcgatgg cgggcgcggc cctcgcgctc acggtttcga
     4021 cgaagaaggc gtacgacttc ccgatgccgc cgggcgccgc cgtcaagggg cgcatcgcgg
     4081 cggtcgacta cggcatcaag acgaacatcc tgcggatgct cgcggcccgc ggcctcaagc
     4141 ccaccgtgtt ccccgcgacc acgcccgcca gggagatcct cgcgcagggt ttcgacggcg
     4201 tcttcctgtc gaacggtccg ggcgacccgg acgcgcttcc ggggccggtt gcgaacgtga
     4261 gggagttcgt cgcgtcgggc aggcccgtct tcgggatctg cctcgggcag cagctcctcg
     4321 gcctggcggt cggcgggacg acgtacaagc tcaagttcgg ccaccgcggc gcgaaccacc
     4381 ccgtcaagaa cctccggacc ggccacgtcg cgatcacctc gcagaaccac gggttctccg
     4441 tcgccgcaga ctcgctgccg aagtacgccg agctcacgca cgtgaacctc aacgacggca
     4501 cgtgtgaggg cttccggctc acggacaagc ccgtcttcgc ggtgcagtac cacccggagt
     4561 ccgcccccgg cccgcacgac tcggacgcgc tgttcgacga attcgtcagc ctcgtcgcgc
     4621 gcccgcgctg acgcgcagcg tccccgaggg gggctgatac gatgtctcaa acgccgcggc
     4681 acacaggagg atctgcatga gcgctcgtgc gcgcacgtct ctcgcctttc ttgcggctct
     4741 cgggctgctc gtcacgccgg ccgcccgcgc ggccgacgga aaggccgagg gcacgatcac
     4801 cgtcaacgga aagacgacga aactcgccca cgcctatgcc cgtgccgtga aaggcttctt
     4861 cgacaagacg aaggaggacg tcgaggtcat cctgagcgac gtccccctgg aggccaaggc
     4921 tctcgaagac ccgttcgagc gcagccgcat ggccgacgcc ggcaagctcc acgccttcga
     4981 aatcacgatc gacgccgacg gcaagccgat ctcgaccgcc ttccgggaca acggtttcaa
     5041 gaaggcgagt ccgagcgggc tttcttcggc cgacgtcttc acgaagaagg tcttcgacgg
     5101 caagacggtg gaaggaagct acaagagcgc gaaggagagc gagttcttcg ggaacaccta
     5161 cagcttcgac gtctccttca aggcgaacgt cgcgcgcgcg cccaagcccg tgcagccgac
     5221 ggcggccgag accgcggccg cccagaaatc gccgcaggcc gcggtctacg ccgatttcct
     5281 gagggccatc cagaaagagg acctcccggc gctcaagaag ctcttcacaa aggagcaggc
     5341 gaagaacctc gacgaccccg aggccaagaa gatggtgggg atggtcaaga tgatgtcccc
     5401 gaccgagatc aaggtcctca gggtggtcga gacgggcgac aaggccgacc tcaccgtcac
     5461 gggcaagcag gacggaagcg tcgcgagcgg cgtcgttcac atggtcaagg aaggcggcgc
     5521 ctggaaggtc cagcgggagg agtggaagaa ctagaccgta agatccgccc gtgcacgtcc
     5581 ccgctttcct cgcgaaacgg ttcctcgcgg gttcgaggcg gggacttccg aagacggtct
     5641 ccgtcctcgc gctctgcggc gtcgcgctcg gcgccgcgtc gctcgtcgtg gcaatgggcc
     5701 tcatgtcggg ttaccggcac gatctcgccg agcgcatcgc gggcacgagc gccgagatca
     5761 tcgtgatgcc cgacgcggcc acggttcccg gcgaggcgcg gaaggccctc gaagccctcc
     5821 cgaacgtctc cgcggtcgcc cgcaccgggt tcgcgcccgc gctcctcctg tcggctgccg
     5881 cgcctcaggg agccgacgcg ctcgtcaagg ccctcgatct tccggccgga ctcgcgacga
     5941 cgcgcctcct cgggcgcgtc cccggcgtgg aggccgcgtt cgcctcgctg cgggcgaagg
     6001 gggagacgcc ggtcctcctc ggcgcgggcc tcgcgcggcg gctgggcgcc gccgccggtt
     6061 cgcgcgtcgt cctcgagacc gcgtcctcgg cgctttcggc aggcgtcgca cctccgacgc
     6121 gaacggcgct gaccgtcgcg gccgtcgtgg agacggggtt ctccgaggtg gacgactcgt
     6181 ggtccgttct cgccctgagg gacttcgacc gcatcgcgcc cccggacgcc cgaatgggcg
     6241 tcctcgaggt gaagctcgag cgttcctcgg ggtccacgga gaccgtcgcc tcggcccgcg
     6301 cgctcctcgg tcccggcgcc acggttctgg actggaaagt cctgaaccgt gatctcttcc
     6361 aggccctcgc cgtccagcag acgcttctct tcgtcgtgct gaccctgatc gtcgcggtcg
     6421 cggccgggac cgtcgtgtcg tcgctcgtcg ttctcctcgc cgaaaagacg cgcgacgtcg
     6481 gcgtgctcgc ggccctcggc gcgcccccgc gcgtcatcgc gcggacgttc cggctctccg
     6541 gcctgatcct cggcggcgga ggcctcgtgc tcggcgtcct cttcggcctc ctcgtctgcg
     6601 tgttcctcac caccttccac gtcgtgcgat tcccgcccga gatcgcgaag gtgtactacc
     6661 tcgcgtggat gccgttccgg cccgagccgc tgcacgtcct cgcgatcctc ggcgtcgggc
     6721 tcctcctcgt cctcgccgcg tcggcgctcc cggcgcggcg cgccgcgcgc ctgaacccgt
     6781 cggacgcgct ccggtacgag tgacgtgcgc ctccgaggct cctcggaacg ggggccctgg
     6841 cgtagggccc cccgtcagcg cggcttcgac tcgcgtcgca ggcgccatac ggtggcgtgg
     6901 gtccgcgcgc cgaaggccag gatcttcccg tcggccgtcc agccggggta ctgcgcgtcg
     6961 aggaaggacg gcagggtgat gcgctgcgcc gcgcccgact ccgggttcag gaccgcgacg
     7021 taccagggcc acgtgtcgct cgcgttcgag gtcttgagga tcgcgccgtc ggcgcggacg
     7081 gcgttggacg tcatcagcac ggcggcgggg cggacaccgg ggaaggacag cggctgctcg
     7141 gggccgcccc ccacgggcac gcgcatgagg cgcacggtgc ccttctcgtt gagctggacg
     7201 acgaggtgtt ccccgcgggg atccgcggcg acgctgtcgc cctcgcagac gggcttcggc
     7261 gggcctccgg ccgctggaat cgcccagatt ttccctctcg ctgcgtagta cagagtccgt
     7321 ccgtccgggg acgaggccag cgcctcgatc gacgggccgt ccgcctcctc cagccggcgc
     7381 acgatgcgcc cgtcggcgac cgacgcgatc gcgacctttc gggccgcggg cgggccggcg
     7441 aggaacgcga cctctgagga tccgaccatc gcgagcggtc ccgaggtctc ctcggccgtc
     7501 tgggcaaacg gcgtcggatt cccccgtggc ctcgccgcga gcaggcggct ccgccggtcc
     7561 acggagacgg tgaaaaggat tcgcccgtcc ggcaggggaa gcgtcggcgt gtacacgagg
     7621 ttgttcacgc tgagaaccgt ctggggccgg ccgccatcgg gctccatcag aagggcgtcc
     7681 tgaggccgga ggacctggtc gacgtagatg tcgccgttct tcgcgacgcc gagcccccag
     7741 accatctgcg tgacggtggt cagcgcgcgc ggggcgccgc cgccgcgcgg gacctcgacg
     7801 atcgccgtga ggtcgcccgc cgggaccgac agcacgatcg accggtcgtc cgcgcctaca
     7861 tccacgacct ggttcaggag cgtgctcctc gagaacggga ggctcgtcga aacgcgccgg
     7921 accgcgcccg agccgagatc gaccgagtag aggtagtcat tgggatcggt ttccggctgc
     7981 tccgtggttc tgccgaggaa gacggcttcc ttgccgtccc ggaacgcgcg cgtcgtgttg
     8041 tcttccgccc cnnnnnnnnn nnnnnggaag agcggtgtag gcctggaggc ggccgccgtc
     8101 cggccagaac cgatagagct ggggctggcg cttctcgttc agccggtgga cgaggaggct
     8161 tccgtccgcg agcacctccg gggacctcgc gtcttccagc acgaggcgct cctcgcctcc
     8221 gaccgcaggg acgctgaaga ctccccgcgg gactccgtcc tgccggccgt agtaaatccg
     8281 cgtcccgtcc ggagaccagc tcatggacgt cgcgacgccg cgcgttcggt cccgggtcag
     8341 gaccgtccag tcgccggagt ccggctgcat gaccgcgacc tgcgtctggc cgtccaccat
     8401 ggcctggaac gcgacgagcc gcccgtccgg cgacggccgc ggcaccatcg cgatctccgg
     8461 gcctcccagc atgacgccgg accagggggc atccggtcgg ctccggctcg agagccacat
     8521 cgcgccgagc gcggccgcga acgcggccgc aagcgcccag gggagaagga acgagagaga
     8581 cttcctgctc cctcgctcac gactcgctcg ttccgcgctt gctacggtgg ggctgggcga
     8641 cgcgggatga agattttctt cgaagggtaa ttgggcggag gagctcgacg acgcggccgc
     8701 gctcaattcg tcgaggtcga ggcgcgcgtc ggcgatgtcg tgcagcctca ggcgcgcatc
     8761 gcgctgcaag caccttctaa gaatcttctt tacggccccc ggcgtctgcg ccggcagcgc
     8821 cgcccagtcg ggctccttcg tgagaactgc cgcgaggacg tccgtgaccg tctccccatc
     8881 gaacgccctc ttcccggtga gcatctcgaa gagcacgcac ccgaacgccc agacgtccgt
     8941 ccgcttgtcg agcggcttgc cgcgcgcctg ctccgggctc atgtacgccg ccgtcccgag
     9001 gatcacgccg gcggccgtcg cgcgcgcggt gagcgtgggg gagtgcgtga cggacggcgc
     9061 cgagcccggc cccgcgtcgc cctcgaagac cttcgcgagg ccgaagtcga ggagcttcac
     9121 gcgcccctcg ggcgtcacct tcacgttcgc gggcttgagg tcgcggtgga cgattccctt
     9181 gtcgtgggcc gcttccaggg cctcggcgat ctggcgggcc agcgaaagtg attcatcgag
     9241 tggaagagga ccggaggaga gacgcaccgc aaggtcgtcg ccttcgacga gctccatgac
     9301 gaggacgtga cgggacgaag gagaagagaa agaggggatt tcttcgaatg aatagatggc
     9361 ggcgattccc ggatgattga gcgccgcgag cagccgcgct tccctctcga accgctgcct
     9421 tctctcttca ccttcaaaga actcctcagg cagtaccttt atcgccacgt ctctcgcgag
     9481 cctcgggtcc ctcgcccggt aaacctctcc cattccgccc gcgccgagcg gcgagaggat
     9541 ttcgtaggga ccgaggcggg ttccggcggc gagtgtcagt aagaaagagt ctaactcccg
     9601 catcagatcg cgccggcaaa gcgcaatcag gcggtttttc cggcgtacac ttcatcgatg
     9661 acgcccgctg cgggaccggt ggtcctcgcc gccgaccacg gcggtttcgc tctgaaggaa
     9721 gcgatcaaga ggcacctcgc cgccgggggc cgggaggtcc tcgacgtcgg gacgagctcg
     9781 acggagccct gcgattaccc cgtgttcgcg cgggccgcag gagaagccgt cgcctcgggg
     9841 cgggcgtggc gggggatcgt cgtcgacggc gccggcatcg ggtcctccat ggtcgccaac
     9901 aagatacccg gggtccgggc ggccatggcg ttcgacccgg cgacggcgcg gaacgcgcgg
     9961 gagcacaacg acgccaacgt cctgacgctc ggggccggtt acctcaagga ggatctcgcg
    10021 ctcgagatcg tggacgtctt tctgtccgcg gactgcaccg tggagcgcca caggcgccgt
    10081 gtcgctatga tcgacgccat cgacaagagc cgcgggagcg cgcccgagga ccagcgccgt
    10141 ccgctcagct cgaggagctc gccgagcatg gcctcacccg acttcgattc cctcgtcggc
    10201 cggatcacgc agatcctggc ttcgaacccc tccctcctcg tgccggcgac gtgcgggggg
    10261 ttcgcggccg gaggcgacgc ctgcacgggc tgcccgatcg gcggccactg cgcgacccgc
    10321 tgcacggaca cggtccgctc gatgatcgcg tgcgtcgggc cgggttgccg cgtctcgggt
    10381 gcgccgggag gcggcgaggt cccgcgcgac ctggcgggtt acatcgacca cacgctcctt
    10441 cgcgccgacg cgacctacgc ggacatcgac aagctctgcg acgaggccgc ccagtacggc
    10501 ttcgcgtcgg tgtgcgtgaa tccggtcttc gtgaagcgct gcgccgaccg cctcgccggc
    10561 atgtcgcccg cggtctgctc ggtcgtgggg ttcccgctcg gggccgcgtc ccgggagatc
    10621 aaggcgctcg aggcgcggcg cgctatccgc gacggcgcgt ccgagatcga catggtcgtc
    10681 gcgatcgggg cgctgaagtc cggtgatctc cgcaccgtgg agaacgacat ccgggcggtc
    10741 gcggaaacgg ctcacgacgg cggtgcgatc ctcaaggtga tcctggagac gtcgctcctg
    10801 accgacgacg agaaggtcgc gggctgcgtc gcggcccgca aggcccgggc ggatttcgtc
    10861 aagacttcga cgggcttctc gacgggcggc gccaccgccc acgacgtgga gctcatggcc
    10921 cgcgccgtcg accgcaagct cggcgtcaag gcgtcgggag ggatccggtc ggccgaggac
    10981 gccgagaagc tgatcgccgc cggcgcgacg cggatcggag cctccgtcgg cgtgaagatc
    11041 gtccaggagg cgcgcgggat cgcgcccgcg tcgaagggcg acgcgaaagc ggcgaagggg
    11101 acgtactgat ggcggacctc cggagcttcg tctacctcga ctcgatgcag ccgcaggtcg
    11161 cggcttacat cggcacggta gcccggggct ttctgccggt cgcccgcgag gcgtccctga
    11221 tcgtcgaaat ctcgcccgga atcgagatca accgcgtcac cgacgtcgcc gtgaaggcga
    11281 ccgacgtccg ccccggcatg caggtcgtcg agcggctcta cggaatgctc gaggtccaca
    11341 gcatctcgca ggccgaggtg cgggccgcgg gcgccgccat cctcgcggct ttgtccctga
    11401 acgaggccga ccggatcaag ccgagggtcc tctcctcaca ggtcatccgg cgcgtcgacg
    11461 ccctccaggc ccagctgatc aaccgggacc ggcagggcat gatgctcgtg gcgggcgagt
    11521 cgctctacat cctcgaggtc gagcccgcgg cctacgccgc cttcgccgcg aacgaggcgg
    11581 agaaagccgc ggagatcaac gtcgtggacg tccggatgac gggggcgttc ggacgcgtct
    11641 acctcggggg cgagcagcgc gacatcgacg tcgcgtcggt cgcggcgatt cgcgcgatcg
    11701 agtcgctcac ggggcgcgag gcgcagaagg gccggaggga ctgacgccgt gatcgaactc
    11761 cggagctacg tctttctcga ctctcttcag gagcagcacg cggcgttcct cggcaccgtc
    11821 gcgaggggtt tcctcccggt cggcgggcag gcttccctct tcatcgaggt ttctccgggg
    11881 atcgcggtga accggctcac ggacgtcgcc gtgaaggcga cctccgtcac gcccggcatg
    11941 cagatcgtcg agcggctcta cgggctcctc gaggtccacc acgacgagca ggcggaggtg
    12001 cgccgcgccg gagaggcgat cctccgggag atcggcaaga gcgaggacga ccggatcagg
    12061 cccgagatcc tctcgtcgac ggtgattcgc ggcctcgacg accgtcagac ccagctcatc
    12121 aaccggacgc gccacgggaa catgatcgtg gcgggtcagt cgctctacat cctggaatgt
    12181 cagcccgcgg cgtacgcggc gctggcggcg aacgaggcgg agaaggccgc gcacatcaag
    12241 gtgctggaga tcgtctcctt cggctcgttc gggcgcgtct acctggcggg gctggaccgc
    12301 gacatcgacg tcgcgcagcc cgcggccgag cgggcggtcc gcggcctctc cgggcgcgcc
    12361 gcgaagtcct gagaagatcg atgaaacgcc gaaccgtgtc gaactcacaa tcggacaggg
    12421 aggatcgaaa tggctgaagc actggggatg atcgagtgcc gctccttcgc cgcgatggtc
    12481 gaagcggccg acgcgatggt gaaggccgcg aaggtggagc tcgtgggcta cgagaagacg
    12541 ggaggcggct acgtgaccgc cgtcatccgg ggcgacgtgg cggccgtgaa agcggccgtg
    12601 gacgccggac agaacggcgc gtcgcgcgtc ggcgacgtcg tgacgaccca catcatcgcc
    12661 cgcccccacg tgaacgtcga cctcgtcctt ccgctcggcc gtgcggccga ggccggcaag
    12721 gagacgggcg cgcacgtcaa gaaatagaga tcggtgaacg ggaggcggtc gggccatgct
    12781 cctggctaaa gtcgtcggaa ccgtcgtggc gtcgcggaag gacccgaaga tcgagggtct
    12841 caagttcctg ctgctccggc aggtgaaccc cgagaacctc agggagggcg ggttcgtcgt
    12901 cgcgaacgac gccgtgggct gcggcgtcgg cgagtacgtc ctctatgcgt ccggctcgtc
    12961 cgcgcgccag accgaggtca cgaaggaccg cccttgcgac gccgtcatca tggcgatcgt
    13021 ggacacctgg gagatcggcg ggcagacgct cttcgaaaag aactcgtccg agcacggggt
    13081 tcgaggcgcc tgagcgtgga gcggcctgaa gcatgagctc cgtgcgcgac cccgggcaga
    13141 tccggcgaat tgccgagatc atcgcgaggg agatcgtcgc ccagacggac gcgggcccgt
    13201 cgcccccttc cgcgggaggc gggtccttcc cgagcgtgga cggcgccgtc gcggcggccg
    13261 ccgtcgcctt tcgcgccctc gacgcggtcg gcctcgagaa gaggcgaacg atcgtcgagt
    13321 cgatgcggga ggccatgcgg cggcacgcgc gttccctcgc ggcgatggcg gtcgaagaga
    13381 ccggcctcgg tcgcgcggag gacaagctcc agaagaacct cctcgtcacc gacaagacgc
    13441 cgggtcccga ggagctgaca ccgtcggcca cgtccggaga ccacgggctc gtcctcgtcg
    13501 agccggctcc gttcggcgtc atcggggcga tcacgccggt cacgaatcct tcctccacga
    13561 tcatctgcaa ctcgatcgga atggtggccg ccggaaacac cgtcgtgttc aacccgcacc
    13621 cctctgccgc gaaggtctcc cgggagacgg tccgccttct cggcgaggcg atcgtgaagg
    13681 cgggcgggcc gccgaacacc gtcacgtgca ccgccgaccc gacgatccag tccgcgggcg
    13741 agctcatgaa gcatccccgc gtccggctcc tcgtcgtcac ggggggcggc gccgtggtgg
    13801 ctgccgcgat gaactccggc aagcgggcca tctgtgccgg gcccgggaac ccgccggccg
    13861 tcgtcgacga gacggccgac gtcgggaagg ccgggcgcga catcgtcttc gggcactcgt
    13921 tcgacaacaa cgtcatctgc acggacgaaa aggaagtcat cgccgtcgac gcgatcgcgg
    13981 acgcgctcaa ggcggcgatg acgaaggccg gggcattcga gcttgacgcc cgcgacctcc
    14041 cgcgcctcga gaacgtgatc ttcgagaagg gggcggggcc tcgcggccac gccgtcgtca
    14101 acaagaagtt catcgggaag gacgcctcgg tgatcctgcg cgagctcggg atcgcggcgg
    14161 gtcccgaggt gcggacgatc ctcgtggagg tcccgaacga gcaccctctc gtctggaccg
    14221 agcagatgat gccggtcctc ccgctgacgc gcgtgaagaa cgtgggcgag gcgatcgacc
    14281 tcgccgtcgc ggccgaagga gcgaacttcc acaccgccac gatgcattcc cacgacctcg
    14341 cggccctgtc gacgatggca cgccggtgca actgctcgat cttcgtgaag aacggcccga
    14401 atctcgccgg cctcggttac ggaggcgagg gccacacgtc tttcaccatc tcctcgccga
    14461 cgggcgaagg gttgacgagc ccccgttcgt tctcgcgctg gcgccgctgc acgctcgtcg
    14521 accacttccg gatcgtgtga cgtgccgatg aatgacgaga ctcccggccc ggcgctcgcg
    14581 atggtccttc tcgacgacgt tccgctgggc ctcgcggccc tggacgtgct cgcgaaggaa
    14641 gcggatgtcg aggtgcttca gacgggcacg atctccggcg gccgctatct gattctcttc
    14701 ggcggcgagg tcgggcccgt ggagcgatcg tggcggcgcg cgctcgacac ggcgggccct
    14761 cgcgtcgtgg acgaggtgtt cctcccccac gccgagctcc ggctcgcacc cgcgatcggg
    14821 acgggggcga tccgctggcc tgctcccggc gacacgctcg gcgtcctcca ggccgcgacg
    14881 ccgccggccc tcgtccgcgc cgtggacgcc gcgctcaaag gcgcgctcgt ggacctcgtg
    14941 gagctgcgtc tcggagacgg tctcggcggg aaagccatcg cgagcctctg gggcgagacg
    15001 cacgacgtgg aagccgcgat cgcgctggcc cggagctcgt tcgagcgggt ggacgcgaag
    15061 ggcgcctcga ccgccgtcat cccgcaggcg gcccgtgccg tccgggaagc gctcgcctcg
    15121 ccgagccggt tcttcgggga gttcctggga tgaaccggcg agggcgtcga tgaagctggg
    15181 aagggtcatc ggcacgctcg tcgcgacggt caagcccgag tcgatgaagg ggctgaagct
    15241 cctcatcgtc aggccgctca cggccgacct tgcggacgac ggcgacccgt tcgtggccac
    15301 ggacgcctcg gcgcaggcgt cgtccggcga cctcgtcacg tgggaggcgt cgcgcgaggc
    15361 ggccctcctt cacgacccgt ggttcatccc ggtcgaccac gccgtcgtcg ggatcgtcga
    15421 ccagcattct ctcgacgacc cggggcggtc ccgatgaacc ggagagccga gccacaggcg
    15481 aggatcgcag gtccctgcgg gggccgagct gaaaggcgag ggcgccgatg atcctcggac
    15541 gcgtcgtcgg gacggtcgtg agcaccgtcc agcacccgtt ctacgcggga aagaagcaac
    15601 tcctcgtcgg agcgatcctg ccggacggga cgctcgacgg cacgaggtac gtcctcgcgc
    15661 tcgacatggt cggcgccggc gtcggccaga cggttctcgt ggaggacgag ggaaactcgg
    15721 cgcgccagat cctggccgac ccgaaggcgc cggtgaggtc gctcatcgtg gcgattgtgg
    15781 acagcgtgga cgtgccggcg tccggcgcgg gctgaggcac gctcagggag tccgaccaag
    15841 aagcgtcgct ccaatgactt aaaatcttga acgtgttcaa aaattagcgt gatattgcag
    15901 ctgcgcggcc tgttttcctg atgtagaatt gaacgcgttc aagttatttc gcgaagggag
    15961 gccgccatga atcgcgcccg gacgcctcgc ggcgaggaga cgcgcgaccg gatccgcgcc
    16021 gccgcgctcg cgctcttcca ggaaaagggc ttcgaggctt cgacgatgcg cgaggtcgct
    16081 tccctcgcgg acgtcgcgac cggggccgcc tactactatt tcccgtcgaa ggaagcgctc
    16141 gtcctcgagt tctacacgcg cgtccaggac gagctcgaga cgacgctccc ggcggccctc
    16201 gaggggcgcc gcgaccttgg cgaaagactg aaagccgtgc tcgaactcaa gttctccctc
    16261 atccggccgt accgccgcgt cctgggagcg ctcgtgcgcg cgggcctcga cccggagaac
    16321 cccgtttccc cgttcagcac agacacggcg ccgctccgcg ggcggcacct cgcgatcttc
    16381 gagcgcgccc tcgagggttc ggaggtcaag atcccgccgg acctcgcgcg gcacctcccg
    16441 cgcctcctgt ggctgtacga catgggtctc gtcctcttct ggctcttcga ccggagcgag
    16501 gggcaggcga gaacggaccg gctgatcgcc cgcagcctcg agacgatcgt cacggcgatc
    16561 catctcgccc gtttccgcgt gacggcgctg ctgaggaagg cggtcgtgcg gctcctcgcc
    16621 gacatcgaag agtctccgca tgacccgcac ctcgcgggcg ccttcgctca gaggtcgtag
    16681 tagagctcga tctcgtacgg gtgcgggcgg ttgcggacct cgcggtcctc ctcgcgcttc
    16741 ttctggatcc agcgcgggac gaggtcggcg gggaagacgc cgtccttcac gagaaactca
    16801 tggtcgctct cgagcgcgtc gagcgactcg tggagcgccg tcgggagcgc gcggatcttc
    16861 gccctctgtt ccgggggcag cgccgcgacg tcgccgtcga acggcccgaa gcccagcgcg
    16921 gtcgggtcca tcttcttttc gacgccgtcg agccctgcga ggagctgcgc ggccatcgcg
    16981 aggtacgggt tgcacgtcgc gtccggcggg cggaactcga agcgcgcgct ttcggggcgg
    17041 ttcgcgtact tcgggatccg gatcgccgcg ctccggttgc cgagcgagta gatcgccgtc
    17101 accggcgcct cgaagccggg gacgaggcgg cggtaggagt tcgtcgacgg gttcgtgaac
    17161 gccatgacgg ccgcgccgtg ctggaggagg ccgccgaccg cgaagcgccc ggtctcgctc
    17221 acgcagccgt agcccttggg atcgaagtac gcgttgcgct cgccgtcgag gacgagctgg
    17281 tggcagtgca tgccgctccc ggcctcgccg tagagcggct tcggcatgaa cgtggccgtc
    17341 agccccatct tcttcgccgc gcgccggacg acgtacttga ccatgaggac ggcgtcgcac
    17401 gcgtcgagaa cgccgcggag cgggatctcg atctcgcatt ggccggggcc gcccacctcg
    17461 tggtggtggt acttcacggg gacgccgaca gactcgagga ggcgcgagat cttcgtccgc
    17521 gcgtcgtcca ggtggtcctg cggcggcagc gcgtggtagc cgccgtgccg cgggatcgtg
    17581 aatccagagc caagttcgtg actccgccag tcggcctcgg acgactcgac gcggtagctc
    17641 gcgacgttga cgccgttttc gtaggcgacg ccgtcgaaga ggtagaactc gaactccggg
    17701 ccccagaggc tgcgcgttcc gacgccgctc gcgcgcaggc gctcctcggc gcgcctcgcg
    17761 atcgttcgcg ggtcgaagag caccggcgag cggtcgtcgg cctcgagaat ccggcagagg
    17821 aagctgagcg tcttctcctc ggggaacggg tcgaggaacg cggtggcgag atccggcacg
    17881 aggacgaggt cgccggactt gacggacctg agtccgacgg acgagccgtc gaacccgacg
    17941 ccgtcctcca tgagctgggg cgtgaagcgc tcggcgggga gcgtcacgtg ccgccaccgg
    18001 cccgcgaggt cgcagaactt caggtcgacc atcgtgacgg cgtgctggcg gacgtgcgcc
    18061 gccgcctcgt cgaattgctg gaacatcggg tcccccggct ccgacgttag gcctcagaat
    18121 gagcctgggc catgggccgt ttgaaccagc cgggcgcgcg cgtgaggagc cggtcgtgaa
    18181 cgagatcccc gacgtctacc gggagctccg ggaacacatc gacgtcatgc cggtcggctt
    18241 cccggcgacg gagagcggcg tggagctctc gatcctccgc cggctcttca ctcccgagga
    18301 ggccgaggcg gcgctcctcc tctcggccct tccggagccg ctcgcgcgga tccaccgccg
    18361 ggcgcccaga gggcgctgga cggccgccga actggaaacc cttctcgacc gcctcgcgag
    18421 caagggggcg atcatgggcg gaggcggcgc cgtgcccggg cgaaagaaga agtggagcaa
    18481 ggcgatgctg gcgctcggca tgtacgagtt ccaggtcgac cggctcacgc ccgaaatgca
    18541 gcgcgacatg gagcgctaca tcgcggaagg cttcgcgggt gcgttcctcg ggccgaagac
    18601 gaagcagatg cggacgattc cggtgaacgc ccggatcgtc gtggagcgcg cggtcggccg
    18661 ctacgacgac gcgcgggacc tgatcgcggg gagcgacggc ccgtgggcgg tcctgaactg
    18721 cgtctgccgg caggggagag acctcacggg cgagccgtgc cgccagacgg acgtccggaa
    18781 gacctgcctc gccctgaagg gcgtcgcgcg ccaccttctc gagacgggct cgggcgagga
    18841 gctgtcgcgc gcgcaggcgc ttgcgctcct cgagagggcc gagcgcgacg ggatggtcgt
    18901 ccagcccgag aacgcgcaga acccgctctt catctgtttc tgctgcggct gctgctgcgg
    18961 cgtccttcgg atggcgaagc agttcccccg tccggcggac gtcgtccact cgaactttcg
    19021 ggccgaggtg ggcgccgagg cctgcatcga ctgcgagtcg tgccgcccgc gctgtccgat
    19081 ggacgcgctc gacaccgtcg gcgggaagac ggcggtggac cacgcgcgct gcatcggctg
    19141 cggcgcgtgc atcggcgtct gtccctccga agcgctgcgt ctcgtcgaga aggagcgccc
    19201 ggccgcgccg ccggagacgc acacggcgct ctacaagagg atcctcgtcg agcggttcgg
    19261 aatcctcgga accgcccgga tgatcggatc ggtgattctc ggccggcggg tctgagagcg
    19321 ctgggccgcg ggcttgcggg cccgtgcgtc agatcagtcc gacggcgcgg gacgcgagga
    19381 ccgccacgac gagcagggag atggccgact ggagcatggt caggaccttc gcccagcgcg
    19441 agaggacggg cgtgtcggtc ggagagaagg ccgtgctgtt gttgaaggcg atgaagagat
    19501 agtccacgaa ccggggggac cagttcctgg gtcccgggca tccgtccatc atctggggga
    19561 agaagaagtc tccctcggtg tgcacgccgc gcccgtcccg ggagtacggg ccgcccgcgt
    19621 cgagcctcca gtaccagatc gcgaagacga gcacgttcgc gatccagagg cacgccgcgg
    19681 attgcgcgag ctccgccggc gtttcggcct tcttcgggag gcccgtgatg aggagcgcca
    19741 gcgagccgac gagggccagc gtcacgacgc cgctcatcgc gaagcccagc gctttcgtga
    19801 gagaggcgcg gcccgcgcgg tgcgcgagca tggtcgggac ggtgaggacg gcgaggaccg
    19861 cgaggacgat ccagcgcggg ccgaggatga gcgtcggcgg cagcgcgaag ttgaggaggc
    19921 cgagcgcgag gagcgcgacg aggaccggcc agcgcgcctc cgacgggatg accttcggcg
    19981 ccgtcacgag ggcatcttac cgagcctggg acgccggtcg gccctgagcg tcagcggaac
    20041 gtgcccaccc agatgtcgct ttcggtgacg tccctcagga aatagagcgc gcgtccgtcg
    20101 ggggagagcg tgatgcgctg gagcgtgccc tgcgcggccg ggaggatgag gcgggactgc
    20161 ttcgtcgcga gatcgaggac gcgaaccccg tcgggagtcg acagcacgac cctccgcccg
    20221 tccggggcga acacgccgac gtaggtgcgg acgccgaggc tttcgcacgg ccccttcccg
    20281 ggcgtgcaga ggtgcgcttc gagcgcgagg tcgccgcccg gccccgccgc gaggagacgc
    20341 gtgctgtcgg gcgaccagct ccacggccag cgggaatcga actccgcgac gggcggaccc
    20401 gcggtgggca ctccgtcctt cgcggtgagc gggaagaccg cgaccttcgt gtcgaaggac
    20461 gccgcggcga cgaagcgccc gtccggggag agcgccggat acaggtagaa gcgcgacttc
    20521 ggaggtgtca ccctcgcgag gccgctgcca tccgccgcaa tcgagtagag gccgtaatct
    20581 ccgtcccgcg tcgagtagaa gacgacgcgc gcgccgttcg ccgtgaaaga cggcgcgcgg
    20641 ttccggaact cgtcgtgggt gagtctgcgg agcttctggc cgtccgacga cacgaggcag
    20701 aggtcctcga ggcggcccac ggtgcgtgtc gcgagcgtgg cgccgtccgg ggagatcgtg
    20761 aggtcctgga aagcgatcga gctgcgtctc aggaccacgg gcgggccctg gggctcgagc
    20821 gaccgcgggt cgagcgccag cttctcgatc gtcatgagct ctgcgggcga cgtgaacgcg
    20881 acgcgcttgc cgttgcggga cccgcggaac gagcccggga aagttcccgc ccacgtgacc
    20941 ggcaccgtga cggactcggg cgcgccccgc gggacgcccg aggcctcgtc gatcgcgatg
    21001 cgccagagat tgaagctccc gccgcggtcg ctcccgaaat agaggaaaga cccgtccgcc
    21061 gaccagaacg ggctgaagtc gacggcggcg tcgttcgtga cggggacggg cttctcgccg
    21121 tccttcaggc ccgcgagcgg cacggtccag acgtcgcgct gcgccgtgtc gccgacgagg
    21181 ccccagaacg cgacgcgctt cccgtgagga gacacggcgg gctcgaccgc gtccgggccg
    21241 tacagcaccc gcttcttgcc ggttgcgacc tcgaccgccc agatcttcgc gcgcgtgccg
    21301 cgcgcaagcg gcgagagcgc cgcctcctcc gcgtagacaa tctccttgcc gtccggcgtg
    21361 aagtctgggc catagcccgt gtccgtcacg cggcgcacgg attcgccgag cggtcccatg
    21421 acgaagatgc cgcctccgtc cttgcccgaa cgaaaggcga tcagcgagcc gtccggagag
    21481 aacaccggat cgaaacggtc gtcctccggg gaatccgccg agaggtcgat ggccttgtcg
    21541 ctgccgacgc gctggacgaa gacgtgccgg tgcgaggaga cggccttcac gtaggcgacg
    21601 gtctcgccgt cgggcgagag ggccggcgaa cgctcgatcc cggggtccga cgagagccgc
    21661 tcgaagagga gcgtcggcgc cggcgcggac ttccggccca tccgtccggc gaggaacccg
    21721 gcggcgagca gggtcgcggc cgcggcggcg aggagagcgc gccgtttgac gaggcgcgac
    21781 ggcgcgacgc ccggtgcgac ggcggcccgc gccgcggtct ccgacgtgag gctctcgagc
    21841 gcgaacgcga ggtcgccggc cgactggaa
//
LOCUS       DAOMFY010000035        21552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_770_length_21552_cov_8.773829, whole genome shotgun sequence.
ACCESSION   DAOMFY010000035 DAOMFY010000000
VERSION     DAOMFY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21552
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_770_length_21552_cov_8.773829"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(549..3320)
                     /locus_tag="PLB01_04960"
     CDS             complement(549..3320)
                     /locus_tag="PLB01_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQQ76682.1"
                     /translation="MAGTTDEMGVRTNYTYDTNGRVETVTRDATGTSPVRFEYAYDPS
                     FPDKVVSVTPKNPTTGSFDPNWQGWKYDYFAGSGTLYHVKRLQNDGASLDTLSTFQYD
                     THGRVLSQTSATGAVTDYVYSGADLFTVTHPTNNDSGSRPVTTYGNYDGVGRPRTITD
                     PAGKDTTYTYDALGRVLTVTLPKPTPAFAGNFTTTYSYDNFDSPSGLAFTHITDPNGR
                     LTKLGYDEHGRLLKSVDAANNTTNYGYTRDVLTSITDANDNVTTYHYDPLKRLDKTTF
                     PDGKFETYTYYADGLLKTKTDRMGQTLTYEYDAFKRLKKKIYPNPAQTITYTYEGQKL
                     KQVDDFYASPAETHMFAYDPSYRVQQNVQSTRGTLNYTYKADDRVDTMTIAGTPNVTT
                     AHTYYPDGSLNTIAWSPQAGQFKYAYTPRGQYQTVTFPNGQTRSYAYDDQGRLLSLGN
                     IGPTATNIATYAYGYDVDWATGLNTMLGQRVSMTANVPSQGFTNAQTKYSYDPLYQLK
                     KAEYPNVTPFNGEVHQWTYDAIGNRLTNQVNASIQTYTYEKIGTNPKNAQKLMNDGVN
                     AYTYDFNGSQTSRTGFGFVNDADNRLAAITGNETASYTYDYQGRRTGKTVGSVTTKYL
                     YDGLNLVSETTAGATTRYAFGPSIDEPLALYASGAISYLNADGLGSIVATNSTAGTVT
                     HGSVFDAWGVAKTETGTRAHSFTYTGREVGEAGLHFYRTRSMQPGAGRFGSEDGLAYQ
                     PGTRSSYAYVDGTPTTYRDPLGAASEVPIGVYTTEWAHSFKLLGRFLLGTGPNYYGYE
                     PTNSRTLAIKNSPGVEAAREKYRKDGCVGLSEFEYKFGLSGLLSSGLNAVRQQVGGYW
                     VWVTPLQHGYAKFTVTNSLTAKSLLYHVDIPENFPRNGPSGPLSRAQPLTDVHEVFSW
                     VEYLPCCEK"
     gene            3352..3477
                     /locus_tag="PLB01_04965"
     CDS             3352..3477
                     /locus_tag="PLB01_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76683.1"
                     /translation="MPEPSPGGGVRFVIWPLGAVVNVDVCPEEFVFVVVVGPTYV"
     gene            3801..4034
                     /locus_tag="PLB01_04970"
     CDS             3801..4034
                     /locus_tag="PLB01_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HQQ76684.1"
                     /translation="MDAGLIVSDPAVMMGKPVVVGTRITVELILEKLAAGETMDQILA
                     AHPRLTREALQAALDFAARALKADVVYPPPSEG"
     gene            4031..4390
                     /locus_tag="PLB01_04975"
     CDS             4031..4390
                     /locus_tag="PLB01_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HQQ76685.1"
                     /translation="MIFLADESVDRVVVSALRAADYDTTYVAEERPGITDSAVLDAAI
                     AENRILITADKDFGDLVFRQARPSPGVLLLRLAGLSPARKGALVVAYVRAHTRQLRGA
                     FSVLLPGGIRIRPSLIP"
     gene            4410..5678
                     /locus_tag="PLB01_04980"
     CDS             4410..5678
                     /locus_tag="PLB01_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HQQ76686.1"
                     /translation="MRTSQPPIGGSSGFAYSRPVDSAALPALLHATHLLGLALGSALG
                     KLRESGATAAKMFEKAEENALLLRMMREAVGILGERWDKVPDRNRPHYEPEQRFRILR
                     IRSFLGLSQRETAEMFRVSKETIARWESEATSSPEDAARSLVSPNPPVRRFADVVRML
                     VKTMELAGFGGNDLIARTLARAGWKLSSRTVGRIRKERRPRPRVPEVSSAAGRAVRAR
                     RPNHVWMVDLTDVRGLFEIVTFKIGVVFDVFSRMPLSELVFSKEPSSAEIARFVSKAA
                     KRHGRPKHFVSDRGPCFIGRVFRQTLKRLGVRPRYGAIGKKGSIALIERLWRTLKDAV
                     HLRGLRPLVAEDLAATIELGLLHYAYFRPHQGLNGATPAEIYFGRTPAHLSAIPPPRG
                     RPGEGPMDSPLRIEYLDAERLLPVLVRNAA"
     gene            complement(5966..6478)
                     /locus_tag="PLB01_04985"
     CDS             complement(5966..6478)
                     /locus_tag="PLB01_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76687.1"
                     /translation="MRVIIGALLVCIASDRGAATEPTGADKIAKTQQRIASIAARVLP
                     PGGPWTVHPGQTYGPESQYAWRIEEGDARGEQAQVLARAFASAEEASAYLEKMNDSSQ
                     MRAVTKCKDITEQCLTLAGGSSTARTCRSYRVAMDNVFLALSGCDREDKVAQAFVKMW
                     VAELKKETEK"
     gene            complement(6491..16666)
                     /locus_tag="PLB01_04990"
     CDS             complement(6491..16666)
                     /locus_tag="PLB01_04990"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQQ76688.1"
                     /translation="MTPTSTPTITPTSTSTQTSTQTPTQTPTQTPTQTPTQSATATPT
                     QTPTQTPTPTPTQTPTATPTPTATPVSSITGTVTNGALSGPVAGALVTAIGTAMSATT
                     DGSGAFEIPVPPGTYSLIITAPNLQPATTGSLVVSAGWSTNAGTIALGAAQTFAFGIK
                     PAIASGNGTATATINLNTAAASTTVFTLTSDSANLSPPASVTIATGKRALSFPVTYAP
                     VSSPTWVTVSATSGSVTNQARVMLTPTLAVASMSLSASSVHLPTAFSGVVQLTAPAPA
                     GGVTVGLRSSVTTAATVPASIFIPEGAYRGLFYGQTLAVAANATTQITASLPSGSVTQ
                     PVFVSTAPALTAVSAPAYARYTASPPKTVSIAVYLNDRAPAGGAVVSLTSSNPTAIPV
                     PASVTVSSCCSSVVTAPVLGATTSSPVVITANYAGISRTTSVTVTAPITAQKMFLGAP
                     LGVSTYVYLEVSEPLPSGGTATFQNSNPSVFSLPTSSPIASGDNEAYPTGTYLPTTYG
                     TQVVLTGSYGSRDQQITVDVPSATSPSGVSVGTSVQGGFSGTGSVALTYPAPAGGVLV
                     ALASDQPSVLSMPPSVTVDAGSSSATFTFSASVVPVSTSARVFATSGGDTQVSSVTVL
                     PPRVASVSVAPTSTPGGNTVAGTLTLTGPAPPEGYVVNISSNSGLALPPPTVTVPGGA
                     TAMTFPIPTAGATASTTSATLTASDGFSTQTTSLQITKTSLLSVTFSPSTVQGGSPST
                     GTVVLNGLAPAGGAVVSLSSNQGSAVVPASVTITAGNASGTFTVTTSAVASNTTAVIT
                     ATYQGFTQPGSLVVTQLPSTLSSIYVPPAVSGGLNATGTVYLSTAAPVGGLPVALTSA
                     LPAVAAVSTSVTVPAGALSANFTITTTGVAVSTPVVISATYAGVTQNGTITVNPASLS
                     SVSLVPFALTAGSSSTGTLSLVGKAPPAGLVISLASSNPGVATVQSTVTVPSGASTVT
                     FPVTTNGSISSGTSTITANGGYQGLTVSALLTVTPSPIALSQLTLSAPTAVSGQVVTA
                     TVLLNQAAPVGGVVVLLASSDTTVATVPSGVVVSAGATSATASVTTKGVGASSPVTIS
                     ATFNGATLPAALTVTPAVLSQVTVTPAAVAGGASASGVVNLSGPAPPAGAVVALSSSD
                     ATTASVPASVTVASGAWGAAFTVTTTTVSVSKPITITAVYSGVTLTAPLSVDPFGVAS
                     ITAPANMKAGDFGVSGTVTLNSPAPPGGSIVSLTAIGAVRYFVSTQTGYLASANTTVT
                     VPAGQTSEYFNVIAPWTSSAGSGTLTATLNSVSKSTSVTVTAGTATFSIAPSTLSRGG
                     STLGSILFDGYPPITNSIPVTLSSSNSGVVLAATSVQACPVLPCGAIGSIQAGYPWYM
                     PIGVTAGSSTGATTVTATFSGLSQTAALAVNPSVLTSLTARPTAFPGGASVPITANLD
                     SPAPPEGALISLTSSSLSLFPVPATLSIAPGQQTASLNAIPQSVGATTPITLSGTYGP
                     DKRNVSLKILADGAAHVFGTVVDARVYDHDQPLPGVSLAFSTDAANATTTDAFGAFSL
                     FTDPGATALTLSSPGFATTTLPNFTAALNGAIDEGVIRLKYVLGGSTLVSGRVVNAGG
                     SPVAGATGSLEGYQGSFTTDGSGYFSFNGPNLGRYRLTFSAPATPTRTDDTFVFVSTA
                     TTSGLYDFVLDSTSRPVLSAVSVSPNPVVGGGANTTLNLTLSAPAPSSPAPGGATVSL
                     VMSDPNATPPYTSADISAGSATGGFVLQVNTVTVPTPTVITAFYGGVQLSTTLTVIPA
                     TGVLSSFTVNPSSVVGGSTSVGTVTLNGPAPAGGVTVGLSSDDGAAIVPSGVFVPAGA
                     TTAAFTMATSLVGLTRTANLTATYGYTRTATLTITPPAQASLIGVAPGWTLPGDMTPV
                     LYGSSIQPGSTVTFTGPVYALAAPTTPLCTLGSNCPSAALAATVDVGGAYAAFAIPPG
                     AAPGFYHLKTRSPVGVDSTNNEWIAVDSAQQTRGVVAPGQHTFAQRIYPGQTVTGTLT
                     GDNPQGSVSDYNYYYFVATAGSQISASMNRVDTSMTWENPASLDPQIQIIAPDGFIYS
                     NLQAFDNQPGVDLNASLSHAVLPQTGVYFIVAETTRGSGQYQLSFNVDSMAPAPAGNR
                     AIPVTGNHNTLPLNTNAGAQAMMLDSRGWPVAGAQYSFSGQNGTGDTGTATFQSGASG
                     STSLNGTADATVRMTTQGKARFKAALSSPQLSQLVYLPSGEAAAAMGGVFDEASGDLK
                     IPVYQATGFSSARLLSLSDKGAQLSAGGIQSLPQEQLRGRVESLSVKGARVAQGAASA
                     NEHSPGVPLRSATTPLAYVRTPLVITACSGELEVFTQSGVTPATLNAPFSVVLTDVTV
                     GEAQGVVDTAAGIHGHKIEKADGTSKVMRFHLDVKDSTGNAPTHPVLVNLAVGGAKHG
                     TLILDPTGAKIECAQASFIWHEQDAQGTIIAPNEEFEYRLGTLSIYAGAVPDPAHSGQ
                     VLPVWGVAENLTVTISTVDGTGNATPLTTYDASFPVHPEPGRPKSFVSPFELVGGPPD
                     AAWQYWADYLTTTVAGVKQSPRYSIPNSFYLVDRWNNVTFGYPVDPAHTTVFGQQNLS
                     VTFKDQLAGVNQPGYDITFPGYEVDLSWSNAGGSMPTGADLSFGLHYSGDPTYGTGDI
                     APPRSVRLNFISGTQHALTSQQDYELRFGVDDTGWPVTVPPGATGSALPTTQDTRNTD
                     PTLSTVPKRLTFQIVTGTNVPWAGEIYETPHNAYDALGNFAQVQYPDPHLETSEPGRM
                     KLSVIESTGAAVTDVAFKVHNCPRYDHEGSPRQPTDMTNVRACSDTPVQSTGGAIASY
                     SVNPAGSSRGYMGVELLQAPTNPGNYYIKIESIDSIYRIKQLPGLTVDATPAGQFKGA
                     FPFVRVNSLELLDASFMRLRAMDPAIYVNASVPLYLSYVAPSSTAAALQGTIDIERLD
                     LTGGGPQAITLTKAGASNRYVAGFTLSRPQAQVRGPAPQAGFASLSVAYPKALFVGAI
                     TGGPNVSHLVRTVSVSVASVGFMNDFKLYNYPVDTPIDPLDTDPLWQAGTSCGAYCGS
                     LDGAKGAAYLANTSPAIFASFNLVPPVAPTEGCYARIRVTDLRPPTSSDPTPAAVVAS
                     GDLFLLNDVAQIRDLRFSAPWSYNKVITTSTFRWEVDYWGNGVWEAAGESGGHKFYWL
                     PGAPVNTPFKNAPDHVYEAALQIALDNHGYNDLPDRSDMALRVNNLVHRQIQFNPQLP
                     MPDGNPLRVWLSAVATQYPAEAEAELLRGLLRSIGIQAETKFVWMGTPATAWVYEFDD
                     TQRGAIDASFQCDLPSMGESRAQPHYIYHVLVEAGQQYDPTYDMHGRLFDDLAILEWW
                     DVLIPGLSSDGSKAVKSIDTVKPTPHILKDANDKRIVCPHH"
     gene            16608..16811
                     /locus_tag="PLB01_04995"
     CDS             16608..16811
                     /locus_tag="PLB01_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76689.1"
                     /translation="MDVWVEVDVGVIVGVDVGVIVRVTVTVDVGVIVGVCVAVTVGVC
                     VAVGVTVDVFVGVTVGIEVGVHV"
     gene            16986..17654
                     /locus_tag="PLB01_05000"
     CDS             16986..17654
                     /locus_tag="PLB01_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76690.1"
                     /translation="MTVGVGVTVGVTVDVGVCVGVLVAVGVGVFVGVTVRVGVVVGVD
                     VGVGVRVAVDVNVAVRVGVGVLVGVGVRVGVGVGARTDRDAAAEACCLTAVMETLVVG
                     AVSGVETVNVAVAVPAATVTLEGTVAAAVLELARYTISPPAGAGPLRVTVPVEVVPPT
                     TVVGLSDTLEGRIDVVVRTAVFVAPETAVTVVSRSSATATVVAENVANSSASRSPRLS
                     EPGR"
     gene            17651..18223
                     /locus_tag="PLB01_05005"
     CDS             17651..18223
                     /locus_tag="PLB01_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76691.1"
                     /translation="MSCTVAIGLCPLVTLVRSRVTEKGCRAAAIVCVAVLDVPPAAAV
                     MTALPLPVSVETVKVAEGAPPDTTMLEGTVARAVFELVRVTVVPPVGAAPLRVTVPVE
                     VEDPNTVVGLRVRVYGIALAEVVSATVLFVPAFEAVIVDEPVVAAVRVAATNVANVAP
                     AGTVTLDGTVALVVAELVSVTTVPPEGAGA"
     gene            18344..18592
                     /locus_tag="PLB01_05010"
     CDS             18344..18592
                     /locus_tag="PLB01_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76692.1"
                     /translation="MIVAVWLVMGAEVVTVKVAVLEPARTVTDKGTEAYALLLERPTT
                     TSPAGATTSSVTVPVAFAIPPTTLVGATETEERFAADA"
     gene            complement(19059..19556)
                     /locus_tag="PLB01_05015"
     CDS             complement(19059..19556)
                     /locus_tag="PLB01_05015"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HQQ76693.1"
                     /translation="MASFDWNLDGSGFGQQTAESCWFAAYAILWSWKNRPLGSIRERI
                     TKAGLDFDDYYGKGLPVEAFPKTRAALGLVGWGGGHAAALAGDLDDMAHRIKGYGPLW
                     CAVSRPSAHVVVLVGADPGGGLIHILNPWSTNGLDAESQYLTAQQFRHKVNAKAPYSV
                     IQAFM"
     gene            complement(19598..20800)
                     /gene="lhgO"
                     /locus_tag="PLB01_05020"
     CDS             complement(19598..20800)
                     /gene="lhgO"
                     /locus_tag="PLB01_05020"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HQQ76694.1"
                     /translation="MKTYDVAVVGGGIVGLATAWRLLEKDPRLSVVLLEKEGEAGAHQ
                     TGHNSGVIHSGLYYKPGSEKAKTCVEGGRLLVDFCRTYGVPHEICGKLVVATSAEELP
                     RLDELARRGRENGVAGVREMEPAEFRKIEPEAAGVKALHVPPTGITDYGVVARTLAAE
                     LTKKGVDVRFRSKVTTLASNGSEVRIVAGGEHVAARHAVACAGLQADRVARLDGVDAG
                     VKIVPFRGEYYELAPGREGLVKGLLYPVPDPRFPFLGVHFTRLVGGGAEAGPNAVPAL
                     AREGYTKTSCNLFDALEEAANPGVWKLLVKYGGMAAGELYRSFWKPAFVRALQKLMPA
                     IRSEDLVPGGAGVRAQALRRDGTLEDDFVLAPHGRIVHVLNAPSPAATASLAIGGRIA
                     EAVAAMRS"
     gene            complement(20811..>21552)
                     /gene="radC"
                     /locus_tag="PLB01_05025"
     CDS             complement(20811..>21552)
                     /gene="radC"
                     /locus_tag="PLB01_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQQ76695.1"
                     /translation="AGAGGRAGAGGGVSAGRGPRGGRLAERSKEERPREKMLRKGPEA
                     LDDEELLAILLGTGTAARPVLETARDLLKDGGFPGLFARGARDLEKLASGVGPAKATR
                     IEAALEIGRRLSLDSLSKKNLLSDPATVGRYLMERLASETQEVMGGLLLDAKNRLVKD
                     AALFRGTVTHAAVAPAPLFRQAILAGAAGVILYHNHPSGDPEPSPEDLATTKRFLAAG
                     REIGIEVKDHVIVGRGRWVSFHERGLLR"
BASE COUNT         3490 a         6674 c         7536 g         3852 t
ORIGIN      
        1 cgccgtcgtt ctggaggcgg acgacctata gtagaaaaca gagacctgac cccaacagcc
       61 ccgcgcagac ctgaccccga agagctccct ttacttgtcg acggccctaa cgaaatagta
      121 cgaagtgcga gcgtccggat cccccagacc gcgtcgaaga cgccagccgt catgtgctga
      181 gacaacttca aaaacaacaa cggtttcgag cggttgctcc cccagagtct gaacatgtag
      241 gctcagctct ggcccggcca gtgcccacgt acccgtgcct ttcaaaccgc gccctagcgt
      301 tgcatcgtga ccgccgagcg caatcggcag tgcttgaaag tccagcgccc cgccctctct
      361 caagttgatc gttggtacgc ccccactcag ggcagagacg cggccaaaca gtgcaccgtc
      421 gtcactcgaa aggcgccatc ggccgataag gtcaccagtt cgcgccgccg gccgacaaga
      481 gatgctccag ggtatcaaga gcgcgctcac cgccagtgcg cgccaagatg ccgagcgcca
      541 acctgagctt acttctcgca gcacggtaag tactcaaccc aggagaaaac ctcgtggacg
      601 tcggtgaggg gttgcgccct agaaaggggc ccagacggcc cgttccgcgg gaagttctcg
      661 ggaatgtcga catggtagag caacgacttg gccgtcaggc tattagtaac cgtgaatttc
      721 gcgtatccgt gctgaagcgg cgtgacccac acccaatagc cgccgacctg ttgacgaacg
      781 gcgttgagcc cggacgacag gagacccgac agtccaaact tgtactcgaa ctcgctgagg
      841 cccacgcagc catccttgcg gtatttttct ctagcggcct cgactccggg agagttcttg
      901 atcgcgagag tccgggaatt cgtgggttcg tagccatagt agttcggccc ggttccgagc
      961 aagaaccgtc cgagcagctt gaagctgtgt gcccattccg tcgtgtagac gccaatcggt
     1021 acttcgctcg ccgcaccaag tgggtcgcgg tatgtagtcg gggtaccgtc aacataggcg
     1081 tacgacgacc tcgttccggg ctgatacgcc aggccatcct cggaaccgaa ccgtcccgct
     1141 ccaggctgca tcgaccttgt cctatagaag tgcaggcccg cctcgccgac ttctctcccc
     1201 gtgtaggtga atgagtgcgc cctcgtaccc gtctccgtct tcgcgacgcc ccaggcgtcg
     1261 aagaccgagc cgtgcgtgac cgtccctgcg gtagagttcg tcgcgacgat cgaccccagc
     1321 ccatcggcat tcaggtaact gatcgctccc gacgcgtaga gggccagtgg ttcatcgatt
     1381 gacggcccaa aggcataccg tgtcgtcgct cccgccgtcg tctccgaaac taagttcagg
     1441 ccgtcgtaca ggtacttcgt cgtgacgctg ccgacggtct tgccagtgcg ccgcccctgg
     1501 tagtcgtacg tgtagctcgc cgtctcgttc ccagtgatcg cagcaagccg gttgtcggcg
     1561 tcgttcacga agccgaagcc cgtcctgctc gtctggctgc cgttgaagtc gtacgtgtaa
     1621 gcgttgacgc catcgttcat cagcttctgc gcgttctttg ggttcgtgcc gatcttctcg
     1681 tacgtgtagg tctggatcga ggcgttcacc tggttcgtca gcctattccc gatggcgtcg
     1741 taggtccact gatggacctc gccgttgaag ggtgtgacgt tggggtattc ggccttcttc
     1801 agctggtaga gggggtcgta ggagtacttc gtctgggcgt tcgtgaagcc ttggctcggc
     1861 acgttggcgg tcatcgagac ccgctggccg agcatggtgt tcagtcccgt ggcccagtct
     1921 acgtcgtagc cgtaggcgta cgtcgcgatg ttggtggccg tgggtccgat gtttccgagc
     1981 gagagcagcc ttccctggtc gtcgtaggcg tagcttctcg tctgcccgtt cgggaacgtc
     2041 acggtctggt actggcccct cggggtgtag gcgtacttga actgtccggc ctgcgggctc
     2101 caggcgatgg tgttgagcga gccgtcgggg tagtaggtgt gggcggtcgt gacgtttggg
     2161 gtgccggcga tcgtcatggt gtcgacgcgg tcgtcggcct tgtaggtgta attgagcgtc
     2221 ccgcgcgtcg actggacgtt ctgctggacg cggtagctgg ggtcgtacgc gaacatgtgc
     2281 gtctcggccg ggctcgcgta gaagtcgtcc acctgcttga gcttctggcc ttcgtacgtg
     2341 tacgtgatcg tctgggcggg gttggggtag atcttcttct tcaggcgctt gaaggcgtcg
     2401 tactcgtacg tgagggtctg gcccatccgg tcggtcttcg tcttcaggag gccgtcggcg
     2461 tagtaggtgt acgtctcgaa cttcccgtcc gggaaggtcg tcttgtcgag gcgcttcagg
     2521 gggtcgtagt ggtaggtggt gacgttgtcg ttcgcgtcgg tgatggaggt gaggacgtca
     2581 cgggtgtagc cgtagttggt ggtgttgttg gcggcgtcga cggatttcag gagccgcccg
     2641 tgctcgtcgt agccgagctt cgtgaggcgg ccgttggggt cggtgatgtg ggtgaaggcg
     2701 aggccagagg gcgagtcgaa gttgtcgtag gagtaggtcg tcgtgaagtt gcccgcgaaa
     2761 gccggcgtgg gcttgggcag ggtgacggtg aggacgcgac cgagggcgtc gtaggtgtag
     2821 gtcgtgtcct tgccggccgg atcggtgatg gtgcgcgggc ggccgacgcc gtcgtagttt
     2881 ccgtacgtcg tgacgggccg ggagccggag tcgttgttgg tgggatgggt gacggtgaag
     2941 aggtcggcgc ccgagtagac gtagtcggtc acggcacccg tcgccgaggt ctgcgagagg
     3001 acgcggccgt gcgtgtcgta ctggaacgtg gagagggtgt ccagcgaggc gccgtcgttc
     3061 tggaggcgtt tgacgtggta gagggtgccc gagcctgcga agtagtcgta cttccagccc
     3121 tgccagttcg ggtcgaagga gccggtggtc gggttcttcg gcgtgacgga gacgaccttg
     3181 tccgggaagg acgggtcgta cgcgtattcg aagcgcacgg gtgacgtgcc cgtcgcgtcg
     3241 cgcgtgacgg tctcgacgcg gccgttcgtg tcgtaggtgt aattggtcct cacgcccatc
     3301 tcgtcggtcg tgcccgccac ggagccgtcg gggtagtagt cggtgtgcgc ggtgcccgag
     3361 ccgtccccgg gcggcggcgt gcggttcgtg atctggccgc tgggcgccgt cgtgaacgtc
     3421 gacgtctgtc cggaggagtt cgtcttcgtg gtcgtcgtcg ggccgacgta cgtgtaggtg
     3481 tacgtctcga ggttctccga gtagctcttg gtccggtcgc ccgcgtcgta cgtgacgacg
     3541 gtgaggacgc ggttccagtt gtcggagatc tgggtcagga gcgggccgta gctgccgttc
     3601 gtgtacccgt agtgcgtgac gcggctggcg gggtcggtga cggtggtgag cgtgccgtcg
     3661 gcggcgtagc cgtaggtgac gcgccggccg gccgagtcct ggatccagtc gatcttcccg
     3721 tcccctctcc accagacgtt gatgtaccgg cccgagccgg cctggtcggc cagagccggg
     3781 atcggctaac atccttaccg atggatgctg gcctgatcgt ctccgacccc gccgtgatga
     3841 tgggcaagcc cgtcgtcgtc ggcacgcgca tcaccgtgga gctgatcctc gagaagctgg
     3901 cggcgggtga aacgatggac cagatcctgg ccgcccaccc gcgattgact cgggaggctc
     3961 tccaggcggc cctcgacttc gccgcgagag ccctgaaggc cgacgtggtc tacccgccgc
     4021 cctccgaggg atgatcttcc tcgccgacga gagcgtggac cgtgtcgtcg tcagcgcact
     4081 ccgggcggca gactacgata cgacgtacgt tgccgaggag cgccctggca tcacggacag
     4141 cgccgtcctc gacgccgcga tcgcagagaa tcggatcctg attactgcgg acaaggactt
     4201 cggcgatctc gtctttcgcc aggctcgccc gtctcccggc gttctcctct tgcggctcgc
     4261 gggtctctcg ccagctcgaa aaggcgcgct ggtcgtcgcc tatgtgcggg cgcacacccg
     4321 ccagctccgg ggtgccttct cggtgctttt gcccggggga atccgcattc ggccgagcct
     4381 tattccctga taccggcgca ggagccccca tgcgcacttc tcagccgccc atcggcggca
     4441 gttctggttt cgcctacagc cggcctgtcg attcggcggc cctccccgcc ctcctccacg
     4501 ccacgcacct tctcggcctc gccctcgggt cagcgctagg aaagctgcgc gagtcgggcg
     4561 cgacggccgc gaagatgttc gagaaagccg aggagaacgc ccttcttctc aggatgatgc
     4621 gcgaggcagt gggaatcctc ggcgagcgct gggacaaggt gccggaccgc aatcgcccgc
     4681 attacgagcc cgagcagcgg ttcaggattc tccgcatcag gagctttctc gggctctcgc
     4741 agcgcgagac cgcggagatg tttcgcgtgt cgaaagagac gatcgcgcgg tgggagtcgg
     4801 aggcgacgag ttctccggaa gacgcggctc gttcgctcgt ttcgccgaat ccgcccgtcc
     4861 gccgtttcgc ggacgtggtg aggatgctcg tcaagacgat ggagctggcc ggcttcggag
     4921 gcaacgacct gatcgcgcgg acgctggcgc gcgccggctg gaagctgtcg tcgaggacgg
     4981 tgggacggat ccgcaaagag cggcggccga gaccgagagt tccggaggtt tcgtcggcgg
     5041 ccgggagggc cgtccgggcg cggcgtccga atcacgtctg gatggtggat ctgacggacg
     5101 tgagagggct gttcgaaatc gtgacgttca agatcggggt cgtgttcgac gtcttctcgc
     5161 ggatgccgct ctcggaattg gtcttctcta aggagccttc gtcggccgag attgcccggt
     5221 tcgtttcgaa ggccgcgaag cggcacggcc ggccaaagca tttcgtgtcg gatcgagggc
     5281 cgtgcttcat cggccgggta ttccgccaaa cgctcaagcg gctcggagtg aggccgaggt
     5341 atggggcgat cgggaagaag ggctcgatcg cgctgatcga gaggctgtgg cggacgctga
     5401 aggacgccgt gcatcttcgc ggactgcggc cgctcgtcgc cgaagacctc gcggcgacga
     5461 ttgagctggg cctactgcac tacgcgtact tccgtccgca tcaggggctg aacggggcga
     5521 cgccggccga gatctacttc ggacgaacgc ccgcgcatct ctcagccatc ccacctccac
     5581 gcggcaggcc gggcgaaggc ccgatggact ccccgcttcg catcgagtat ctcgacgcgg
     5641 agcggctgct gccggtgctc gttcgaaacg cggcctgaag ggcgacacgg cgttcggcaa
     5701 gcgacgctga accgggaaga gatgtgcgct cgccgcttcc gacggcggcg aaaccgctgg
     5761 cccgtacgcg ccaggaggcg ccgcaattgc cattgcgccc ctacagcagt caccgtatgc
     5821 cggcgcggag acaatacgat cagcgacagg acacgtcgca acacgacctg cttcgcaaca
     5881 gcgcggcact atttcgtttc taggagattt gtgatcgcgc catcgcactt cgcacccggt
     5941 acgctcatgc tctcttgccc gcgcgttact tctcggtctc tttcttgagc tctgcgaccc
     6001 acatcttcac gaacgcctgc gctaccttgt cctccctatc gcagcccgac aatgcgagga
     6061 acacattgtc cattgcgact ctgtatgacc tgcatgtgcg agccgtagaa ctgccaccgg
     6121 ctaacgtcag acattgctcc gtgatgtctt tgcatttcgt taccgcacgc atttggctgc
     6181 tgtcattcat cttctccaaa tacgcactcg cctcctcagc cgaagcaaat gcacgcgcca
     6241 gcacctgcgc ctgctcgcca cgtgcgtcgc cctcctcgat cctccaggca tattgcgatt
     6301 cggggccgta tgtttggcct gggtgaacag tccatgggcc gcccggcggg agcacccgcg
     6361 cggcgatgct tgcaatacgc tgctgggtct tggcgatctt gtcggcgccg gtcggttccg
     6421 tggctgcgcc gcggtcgctc gcaatgcaaa ccagtaacgc accaatgata accctcattt
     6481 gacgctcctc ctagtgatgc ggacacacga tccttttgtc gttggcgtct ttcaggatgt
     6541 gcggcgtcgg cttgaccgta tcaatagact tgaccgcctt actgccgtcg gagctcaagc
     6601 cagggatcaa tacatcccac cactcgagaa tggccagatc gtcaaacagt cggccgtgca
     6661 tatcgtacgt cggatcatat tgctgtcctg cttctactag gacatggtag atatagtgag
     6721 gttgcgccct agattcaccc atactcggaa ggtcacactg gaaactcgcg tctatggcac
     6781 ctcgctgagt gtcatcaaat tcgtaaaccc acgcggttgc cggagttccc atccagacga
     6841 actttgtctc cgcctgtata ccgatactgc gcaggaggcc tcttaagagt tcggcctccg
     6901 cctcagcagg gtattgtgtt gcaactgccg agagccatac tcgcagggga ttgccatcgg
     6961 gcatagggag ttgtggattg aactggatct gcctgtgcac caagttgttc acccgcaacg
     7021 ccatgtcgct gcgatcgggt aggtcgttgt agccatggtt atcaagcgcg atctgcaatg
     7081 ctgcctcgta cacatggtcg ggggcgttct tgaacggggt gttcacgggg gcccccggta
     7141 accagtagaa tttgtggccc ccactttcgc ctgctgcctc ccacacaccg ttgccccaat
     7201 agtcgacctc ccagcggaac gtcgaggttg tgatgacttt attgtacgac cagggtgccg
     7261 agaaccgcag atcgcgaatt tgagccacat cgttcagaag gaacagatct ccggaagcga
     7321 cgactgcggc tggggttggg tcagatgaag tcggaggtcg aaggtcggtc acgcggatgc
     7381 gtgcataaca cccctctgtt ggtgcgaccg gaggtaccag attgaatgag gcaaagatag
     7441 caggcgacgt atttgcgaga tacgccgcac ctttcgcgcc gtcgagggat ccgcagtagg
     7501 caccgcacga cgtgcccgcc tgccagagag gatcggtatc tagtgggtca atcggagtgt
     7561 ctaccggata gttatagagc ttgaaatcat tcataaagcc gaccgacgcc actgagacgc
     7621 tgaccgtgcg aaccaggtgg ctcacgttcg ggccacccgt aatcgcgccg acgaagagag
     7681 ccttggggta agccacggat agcgaagcga aacccgcctg cggtgccggc cctcgtacct
     7741 gtgcctgggg ccttgagaga gtgaaccccg cgacgtagcg gttagaggct cctgcctttg
     7801 taagtgtgat cgcctgcggc cccccgcctg ttaagtcaag cctttcgata tcgatggtgc
     7861 cctgcaaagc agcggctgtt gacgatggcg ccacgtagga caaatagagc gggactgagg
     7921 cattgacata gatcgccgga tccatggctc gaagtctcat gaaggatgcg tccaagagtt
     7981 cgagagagtt cacccgtacg aacgggaacg ctcccttgaa ctgccccgcc ggcgtcgcat
     8041 ccaccgtcaa ccctggcaat tgcttgatcc ggtagatcga gtcgatcgac tcgatcttga
     8101 tgtagtagtt ccccggattc gtcggcgcct ggagtagctc cactcccatg tatccgcgac
     8161 tcgagcctgc ggggttgacg ctgtagctcg cgatggcgcc gccagtcgac tgcaccggcg
     8221 tgtccgagca agcacggacg ttcgtcatgt ccgtcggctg cctcggcgag ccctcgtggt
     8281 cgtagcgcgg gcagttatga accttgaacg ccacgtccgt gacggcggcc ccggtgctct
     8341 cgatgaccga cagtttcatc ctgccgggtt cagacgtctc gaggtgggga tcgggatact
     8401 gcacctgagc gaagttccct agggcgtcgt aggcattatg cggcgtctcg tagatctctc
     8461 cggcccacgg gacattagtg ccggtgacga tctggaaggt caagcgcttc ggcaccgtgc
     8521 tgagagttgg gtccgtgttt cgtgtgtcct gcgtcgtcgg caatgcgctg cccgtggcgc
     8581 ccgggggaac agtcacgggc cagcccgtgt cgtcgactcc aaaccgcagc tcgtagtctt
     8641 gctggctcgt gagggcgtgc tgcgttcccg agatgaagtt cagtctgaca ctccgcggtg
     8701 gggcgatgtc gcctgtcccg tacgtgggat cgcccgagta gtgcagtccg aatgagagat
     8761 cagcgcccgt cggcatcgag ccgccggcgt tgctccacga aaggtccact tcgtagcccg
     8821 ggaacgtgat gtcgtagccg ggctggttga cgccggcgag ctggtccttg aacgtgacgg
     8881 agaggttctg ctggccgaag acggtcgtgt gcgcagggtc caccgggtag ccgaacgtca
     8941 cgttgttcca gcggtcgacg aggtagaagg agttggggat ggagtatcga gggctctgtt
     9001 tgacccccgc cacggtcgtg gtgaggtagt cggcccagta ctgccatgcg gcgtccggag
     9061 gcccgccgac gagctcgaag ggcgagacga agctcttcgg tcgccccggc tcgggatgaa
     9121 ccgggaacga cgcatcgtag gtcgtgagcg gagtcgcgtt gcccgtgccg tcgacggttg
     9181 aaatggtgac ggtcaggttt tccgcgacgc cccagaccgg gaggacctgg ccgctgtgag
     9241 cggggtcggg cacggctccg gcgtagatcg agagggtgcc gagtctgtac tcgaactctt
     9301 cgttgggagc gatgatggtg ccctgggcgt cttgctcgtg ccagatgaag gaggcttgcg
     9361 cgcactcgat cttcgcgccg gtgggatcga ggatgagcgt gccgtgcttc gcgccaccca
     9421 cagcgaggtt tacgagtacc gggtgcgtcg gggcgtttcc ggtcgagtcc ttgacgtcga
     9481 ggtggaacct catcaccttg gaagtgccgt cggctttctc gatcttgtga ccgtggattc
     9541 ccgcggccgt gtcgacgacg ccctgggcct ctcccaccgt gacgtccgtg agaacgaccg
     9601 agaagggcgc gttgagggtc gcgggcgtga cgcccgactg cgtgaagact tcgagctcgc
     9661 cggagcaggc cgtgatgacg aggggcgtgc ggacgtaggc gaggggcgtc gtggcggacc
     9721 ttagtggcac gccaggagag tgctcgttgg cagaggcggc gccctgagca acgcgcgctc
     9781 ccttgaccga cagcgactcg acccggccgc ggagctgttc ctgcggaaga ctctggatgc
     9841 cacccgcact cagctgcgct cccttgtccg agaggctgag aagccgcgcc gaggagaacc
     9901 cggtcgcctg gtagacgggg atcttcaggt cgcccgaggc ttcgtcgaag accccgccca
     9961 tggccgccgc ggcttccccg ctcggcaggt agacgagctg agaaagctgt gggctgctca
    10021 gggccgcctt gaagcgcgcc ttgccctggg ttgtcatcct gaccgtggcg tcggccgtac
    10081 cgttgagcga cgttgaaccg gaggcgccag actggaaggt agccgttccg gtgtcgcccg
    10141 ttccgttctg gcccgagaac gagtactggg cgcctgcaac cggccagccg cgggaatcca
    10201 gcatcatggc ctgggcgccc gcgttggtgt tgagcggcag cgtgttgtgg ttgcccgtga
    10261 ccgggatggc cctgtttccg gccggagcag gcgccatcga gtcgacgttg aacgagagct
    10321 ggtactgacc gctcccgcgg gtcgtctccg ccacgatgaa gtagacgccg gtctgcggaa
    10381 ggacggcgtg cgagagagag gcgttgaggt cgacgcccgg ctggttgtcg aacgcctgca
    10441 ggttcgaata gatgaagccg tccggcgcga tgatctggat ctgcgggtcg aggctggccg
    10501 ggttctccca cgtcatcgac gtgtcgacgc ggttcatcga cgcgctgatc tggctgccgg
    10561 ccgtggcgac gaagtagtag tagttgtagt ccgagacgga gccctgcggg ttgtcgcccg
    10621 tgagggtccc ggtgacggtc tggcccgggt agatgcgctg cgcgaacgtg tgctggccgg
    10681 gcgccacgac gccgcgggtc tgctgcgcgg agtcgacggc aatccattcg ttgttggtgg
    10741 agtcgacgcc aacggggctt cgcgtcttca ggtggtagaa acccggcgcc gctcccggcg
    10801 ggatcgcgaa ggccgcgtag gcgccgccga cgtcgaccgt ggcggcaagg gctgccgacg
    10861 ggcagttcga gccgagcgtg caaagaggtg tcgtcggggc cgcaagcgca tacaccggcc
    10921 ccgtgaacgt caccgtgctg cccggctgga tgctcgatcc gtaaaggacg ggcgtcatgt
    10981 ctccgggcag cgtccagccc ggcgcgacgc cgatgagcga ggcctgggca gggggagtga
    11041 tcgttagggt cgcggttcgc gtgtaaccgt aggtagccgt gaggttcgcg gtgcgcgtga
    11101 ggccgacgag agaggtcgcc atcgtgaagg ccgccgtcgt cgcgcccgcc gggacgaaga
    11161 cgccggaggg gacgatggcc gccccgtcgt cggacgagag gccgacggtg acgccgcccg
    11221 ccggcgcggg gccgttgagg gtgacggtgc ctacggacgt gcttccaccc acgacgctcg
    11281 acggattcac cgtgaacgac gagaggacgc cggtggccgg gatcacggtc agcgtcgtcg
    11341 aaagctgaac gccgccgtag aaggccgtga tcacggttgg cgtcgggacc gtgacggtgt
    11401 tgacctgcag gacgaagccg cccgtcgcgc tgcccgcgga aatgtctgcg ctcgtatagg
    11461 gcggtgtggc gtttggatcc gacatcacga gactgaccgt cgccccaccg ggagccggcg
    11521 acgagggggc cggagcggat agcgtcaagt tcagcgtcgt attcgcgcca ccgccgacga
    11581 cgggattggg gctgacagag acggccgaaa ggactggccg ggacgttgag tcgaggacga
    11641 agtcatagag cccactggtc gtggcagtcg acacgaagac gaacgtgtcg tccgtgcgtg
    11701 ttggagtcgc tggagccgag aacgtgagcc tgtagcgccc gagattcggg ccgttgaacg
    11761 agaagtagcc gctgccatcc gtggtgaagc tgccctgata gccctcgagc gagcccgtcg
    11821 cgcccgccac ggggcttccg cctgcgttaa ccacgcggcc gctcacgaga gtggatcccc
    11881 cgaggacgta cttgagccgg atcacgccct cgtcgatcgc gccgttcaaa gcggccgtga
    11941 agttcggaag ggttgtcgtg gcgaagcccg gcgatgaaag cgtgagtgcc gtcgcaccgg
    12001 gatccgtgaa gaggctgaag gccccgaacg catcggtcgt cgtcgcgttt gcggcgtccg
    12061 tcgagaacgc gagggagacc cccgggagcg gctggtcgtg gtcgtagacg cgggcatcga
    12121 cgaccgtgcc gaagacatgg gcggcgccgt ccgcgaggat cttcagcgac acgttccgct
    12181 tatccgggcc gtacgtgccc gagagcgtga tgggcgtcgt cgcacccacg ctctgcggga
    12241 tggcgttgag ggaagcagtc tgctggcctg gcgcaatcga aagcgtcgcg ggcaccggga
    12301 agagcgagag gctcgagctg gtgagcgaga tgagggcccc ttcggggggc gccggactgt
    12361 cgaggttggc cgtgatcggc acagacgcgc cgccggggaa ggccgttgga cgcgccgtga
    12421 gcgaagtgag gactgacggg ttcaccgcta gcgcggcggt ctgactgagg cccgagaatg
    12481 tcgcggtgac ggttgtcgcg cccgtcgacg agcccgccgt aactccaatc ggcatgtacc
    12541 acggatagcc ggcttgaatc gacccgatgg cgccgcaggg aagaaccggg cacgcctgaa
    12601 cggacgtcgc cgccagtacc acgcccgagt tgctcgacga gagtgtcacc ggaatcgagt
    12661 tggtgatggg cgggtagccg tcgaacagga tcgagccgag tgtgcttccg ccccgggaca
    12721 gcgtggatgg cgcgatactg aaagtcgcag tcccggcggt gacggtcacg gacgtcgatt
    12781 tggagacact gttgagcgtt gccgtgagcg ttcccgagcc cgcggacgat gtccatgggg
    12841 cgatgacgtt gaagtactca ctggtctggc cggccgggac cgtcaccgtg gtgttcgcgg
    12901 acgcgaggta ccccgtctgg gtcgacacga agtagcggac cgctccgatc gcggtcaaag
    12961 agacgatgga tccgcctgga ggcgccggtg aattgagcgt cacggtaccg gagacgccga
    13021 agtcaccggc cttcatgttc gcgggcgccg taatggacgc gacaccgaac ggatcgacgc
    13081 tgagtggcgc cgtcagtgtc acgcccgagt agacggccgt gatcgtgatc ggcttcgaga
    13141 cggacacagt cgtggtcgtg acggtgaagg ccgcacccca cgctccggaa gcaaccgtga
    13201 cgcttgccgg cacggaggcg gtcgtcgcgt cggaagacga cagagccacg accgccccgg
    13261 caggaggcgc aggcccgctc aggttgacga cacctgaggc actggctccg cccgccacgg
    13321 ccgccggcgt gacggtgacc tgcgaaagaa cggcgggcgt gactgtgagg gccgccggaa
    13381 gggtcgcgcc gttgaacgtc gcggagatcg tcacgggaga cgaggcgccg acgccctttg
    13441 tggtgacact cgccgtggcc gaggtcgcgc ctgcagagac gacgaccccg gatggaaccg
    13501 ttgcgaccgt ggtgtcggag ctggccagta agacgacgac cccgccgacc ggggccgctt
    13561 ggttcagcag taccgtggcg gtgacgacct ggccagacac agcggtaggc gcgctcagcg
    13621 tcagctgaga cagtgcgatg ggactcggcg tgacggtgag caacgcggag accgtcaagc
    13681 cctgataacc gccgttcgcg gtgatcgtgg aggtcccgct cgagatcgac ccgttggtcg
    13741 taaccggaaa cgtgacggtg ctcgcacccg atgggaccgt caccgtcgac tggacggtgg
    13801 cgacgccggg gttcgaagac gcgagcgaaa tcacgaggcc agcgggcggc gccttcccga
    13861 cgagcgaaag ggtgccggtc gaagacgacc cggccgtcag cgcaaacggc acgaggctca
    13921 ccgagctcag cgacgccggg ttgaccgtga tggtgccgtt ctgcgtgaca cccgcgtacg
    13981 tggccgagat gacgaccggc gtggacacgg caacgcccgt cgtcgtgatc gtgaagtttg
    14041 ccgacaaggc tccagcggga accgtgactg aggtggacac ggccgcgacg gccgggagcg
    14101 cactcgtcag tgcaaccggt agtcctccca ccggcgcagc cgtcgacagg tagacggtgc
    14161 ccgtggcgtt gagaccgccc gagacggcag gaggcacgta gatcgaggac agcgtcgacg
    14221 ggagctgcgt gacgacgagg ctcccgggct gagtgaagcc ctggtaggtc gcggtgatta
    14281 cagccgtcgt gtttgacgca accgcgctcg tcgtgaccgt aaaggtcccg ctggcgttac
    14341 cggccgtgat cgtcacgctg gcgggcacga ccgctgaacc ttgattgctc gagagggaga
    14401 cgacggctcc cccggccggg gcgaggccat tgaggacgac ggtcccggtt gacggcgagc
    14461 ccccctgaac cgtgctcggg ctgaacgtga cactcaggag ggaggtcttc gtgatctgaa
    14521 gggacgtcgt ctgcgtcgag aacccgtcgg acgccgtgag ggtggcgctc gtcgtactcg
    14581 ccgtcgcccc ggccgtcgga atcgggaagg tcatcgctgt cgctccgccg ggcaccgtca
    14641 ccgtcggggg cggcagcgca aggccggaat tcgacgagat attgacgacg tagccttccg
    14701 ggggagctgg cccggtgaga gtcagtgtcc cggcaaccgt attcccacct ggagtgcttg
    14761 tcggcgccac gctgacgctc gcgacccttg gcgggagaac cgtaaccgag ctgacctgag
    14821 tatccccgcc cgaggtcgcg aaaaccctcg cactcgtcga aacaggtacc acgctggcgc
    14881 tgaaggtgaa tgtggccgag ctcgagcccg catcgaccgt gacgctcggc ggcatcgaaa
    14941 ggacgcttgg ctggtcgctt gcgagtgcga ccaagactcc gcctgccggc gctggataag
    15001 tcagcgccac ggaacccgtg ccggagaacc cgccctggac actcgtaccg acggagacgc
    15061 cggacggtga ggtcgccgag ggcacgtcta cggtgatctg ttggtcgcgg gacccgtacg
    15121 agcccgtcag gacgacttga gtgccatagg tcgtcggcaa atacgtgccc gtcgggtacg
    15181 cctcgttgtc accactcgcg atcggggaac tggtggggag gctgaacacc gagggattgg
    15241 aattctggaa tgtggcggtg cccccgctgg gaagaggctc ggacacttcc aggtacacgt
    15301 aggtcgacac gccgagaggc gcgccgagga acatcttctg cgcggtaatg ggggccgtca
    15361 cggtcaccga ggtcgtgcgc gagatccccg cataattggc cgtgatgacg accggtgaac
    15421 tcgtagtggc gccaaggacc ggtgctgtga cgacggacga gcagcacgaa ctgaccgtga
    15481 cactcgccgg gacgggtatg gctgtcggat tcgagctcgt cagagacacg acggctcccc
    15541 ctgccggagc ccggtcgttg agatagaccg cgatgctcac cgtcttcggg ggtgaggccg
    15601 tatatcgcgc atatgccgga gcggataccg ctgtaagagc tggcgccgtg ctgacgaaaa
    15661 caggctgcgt cacgctgcca cttggcaagg acgcggtgat ctgcgtcgtg gcatttgccg
    15721 caacggcgag cgtttgcccg tagaacaatc ccctgtatgc gccctcggga atgaagatcg
    15781 aagcgggcac cgtagccgcg gtcgtaaccg aggatctgag accaacggtc acaccacccg
    15841 ccggggctgg tgccgtcagc tggacaacac ctgagaacgc agttggcaga tgcacactcg
    15901 aagcgctcag cgacatggag gcgacagcca gggtcggcgt caacatcacc cgggcctggt
    15961 tcgtgacgct tccagacgtg gctgacaccg tcacccaggt cggcgacgag accggcgcgt
    16021 aggtcaccgg gaagctcagg gcccgctttc cggtcgcgat cgtgacactc gcgggaggtg
    16081 agaggttggc gctgtcgctt gtcagggtga agacagtcgt actggcagcg gccgtattga
    16141 ggttgatcgt cgctgtcgcc gttccgttgc ccgaggcgat ggccggtttg atgccgaagg
    16201 cgaaggtctg tgcggcgccg agcgcgatcg tccccgcgtt cgtcgaccag ccggccgaaa
    16261 cgaccagact gccggtcgtc gccggctgca gattcggagc cgtgatgatc agtgagtagg
    16321 ttccgggcgg gaccgggatc tcgaaggcac ccgagccgtc cgtcgtcgcg gacatcgccg
    16381 taccaatcgc ggtcaccagc gctcccgcga ccggccccga gagggccccg ttcgttacgg
    16441 tccccgttat cgaagagacc ggcgtcgccg tcggagtggg agtggcggtc ggtgtctgcg
    16501 taggcgtggg cgtgggtgtc tgggtcggtg tctgggtcgg ggtagcggta gccgattgcg
    16561 tcggggtctg cgtgggcgtt tgagtcggtg tctgagtagg cgtttgcgtg gacgtctggg
    16621 tcgaggttga cgtaggcgtg atcgtcgggg ttgacgtcgg cgtcatcgtt cgggtgaccg
    16681 tcacggtgga cgtcggagtc atcgtcgggg tctgcgttgc agtcacggtg ggtgtctgcg
    16741 tcgcagtcgg cgtcacggtc gacgtgttcg tcggcgtcac tgtcgggatc gaagtcggcg
    16801 tccacgtctg agtcggggtc accgtcggcg tcgctgtcga ggtcggtgtc accgtcggag
    16861 tgactgtcgg agtcggggtc gaagtgggcg tcccggtcgg cgtcggagtc ggagtcgagg
    16921 tcggtgtcac cgtgggtgtg acggtcggcg agggcgtgta ggtaaacgtc cacgtcggcg
    16981 tgggggtgac ggtcggcgtc ggcgtcaccg tcggcgtgac cgtggacgtc ggggtctgcg
    17041 tcggcgtcct ggtcgccgtc ggggtcggcg tgttcgtcgg ggtgacggtg cgggtcggcg
    17101 tggtcgtcgg cgtcgacgtc ggcgtagggg tgcgcgtcgc ggtggacgtg aacgtggccg
    17161 tccgcgtggg agtcggcgtc ctcgtggggg tgggcgtccg cgtcggggtc ggagtgggcg
    17221 cgagaaccga cagggatgca gcagcggaag cctgctgctt gacggccgtg atggagacgc
    17281 tggtcgtcgg agccgtgagc ggcgtcgaga ccgtgaacgt ggcggtcgcc gtaccggcag
    17341 ccacggtcac gctcgaaggg acggtcgccg cggccgtgtt ggagctggcg agatacacga
    17401 tctccccgcc cgcgggcgcg gggccgctca gggtcacggt ccctgtcgag gtggtgcccc
    17461 cgaccacggt cgtcgggttg agcgacacgc tggagggccg gatcgacgtc gtggtgagga
    17521 cggccgtctt cgtcgccccg gaaaccgcgg tcaccgtggt gtcgaggtcg tccgcgacgg
    17581 ccacggtcgt ggccgagaat gtcgcgaact cgtcagcgtc acgctcgccc cgcctgtcgg
    17641 agcctggccg ttgagctgta ccgtcgcaat cgggctctgc cccctcgtca cgctcgtgcg
    17701 gtcgagggtg accgagaagg gttgcagggc cgccgcgatc gtctgcgtcg cggtcctgga
    17761 cgtgccgccg gccgcggccg tgatgacggc acttccgctc cccgtgagcg tcgagaccgt
    17821 gaaggttgcc gagggcgccc ctcccgacac gacgatgctc gaggggacgg tggcgagggc
    17881 ggtattcgag ctcgtcaggg tgactgtcgt cccgccggtc ggcgccgcgc cgttgagagt
    17941 cacggtgccc gtggaggtcg aggacccgaa cacggtcgtg gggttgaggg tcagggtgta
    18001 cgggatcgcg cttgccgagg tcgtcagcgc tacggtcttg ttcgtgccgg cgttcgaagc
    18061 cgtgatcgtg gacgagcccg tggtcgccgc cgtgagagtc gccgcgacga acgtcgccaa
    18121 cgtggcgccg gccggcacgg tcacgctgga cgggacagtg gccctggtcg tggccgagct
    18181 cgtgagcgtg acgacggtgc cgcccgaagg cgcgggggcg tagaggttca cggcccccgt
    18241 gaccgccatt ccgccgacga cgctgctgcg ggcgagagtg accgacgaga tgagcgttgt
    18301 gaccgtgagg ttcgcggtct tgttcacggc gttgtaagtg gccgtgatcg tcgccgtctg
    18361 gttggtgatg ggggcggaag tcgtgacggt gaaggtggcg gtcctcgaac ccgcgaggac
    18421 ggtgaccgac aaagggaccg aagcgtatgc gctgttgcta gagaggccga cgactacgtc
    18481 tccggctggg gcgaccacgt cgagcgtcac ggtccccgtg gcgttcgcca tcccgccgac
    18541 gacgctcgtc ggcgccacgg agaccgagga gaggttcgcg gccgatgcct gaggggcggc
    18601 aaggaaaaac gaccacgacg gccccgaaca gaccaagcct cgaaacactt ctcatgtgac
    18661 tcccgttcct gtcgccgtag tgccctggag cgccgcttct tgaccctcgc ctgttgcgat
    18721 tccctgcaac ggaagcgcaa tagcgcccgg acgaacagac ttccaccgag ccggggccgc
    18781 cgaagtgccg atcaagcgcg ccccatccac gccaggttca accggcgact tccgtgggcg
    18841 ggatcgctcc gggcaaactg ccatcggggg ccctggctct aattcaatgg accggagaaa
    18901 aggggaacgc ggggctgact tccgtgaacc tacaccccaa accatgggcg tgcaaggtca
    18961 gtgggtgacc gtatcagccg cgcattcccg ccatggcctt cgcgatgcga ccgccgcacg
    19021 gccgcgtcgt caacgtcctg aacgcgccgt cgccacgcct acatgaacgc ctgaatcacc
    19081 gagtagggcg ccttcgcatt caccttgtgg cggaactgct gcgccgtcag gtactggctc
    19141 tccgcgtcga gaccgttggt gctccaggga ttcaggatgt gaatcagacc gccgcccggg
    19201 tccgcgccca ccagcaccac gacatgagcg ctcggccggc tgacggcgca ccacagcggc
    19261 ccgtacccct tgatccggtg cgccatgtcg tccaggtcgc cggccagcgc cgccgcatgg
    19321 cctccgcccc aaccgaccag gcccagggcc gcgcgcgtct tcggaaaggc ctccaccggc
    19381 aagcctttgc cgtagtagtc gtcgaaatcc aatccggcct tcgtgatccg ctcgcggatc
    19441 gacccgagcg gtctgttctt ccacgaccac aggatcgcgt aggccgcaaa ccagcaactt
    19501 tcggcggtct gctgaccaaa ccccgacccg tccagattcc agtcaaagct ggccacggcg
    19561 cgaattgtat gcccgcagcc gtccccgaga ggcgcgctca gctccgcatc gccgccaccg
    19621 cctccgcgat gcggccgccg atcgccaggg aggccgtcgc ggcgggtgac ggcgcgttca
    19681 ggacgtggac gatgcggccg tggggcgcga ggacgaagtc gtcctcgagc gtgccgtccc
    19741 tgcggagcgc ctgcgcgcgc acgcccgcgc cgccggggac gaggtcctcg ctccggatgg
    19801 ccggcatgag cttctgcagc gccctcacga acgccggctt ccagaacgag cggtagagct
    19861 cccccgccgc catgccgccg tacttcacga ggagcttcca gacgccggga ttcgcggcct
    19921 cctcgagcgc gtcgaagagg ttgcacgacg tcttcgtgta gccctcgcgc gcgagcgcgg
    19981 gaacggcgtt cgggcccgcc tcggcgcctc cgcccacgag ccgcgtgaag tggacgccca
    20041 ggaacggaaa gcgcgggtcg ggcacgggat agaggaggcc cttcacgagc ccctcgcgcc
    20101 ccggcgcgag ctcgtagtac tcgccgcgga acgggacgat cttcacgcca gcgtcgacgc
    20161 cgtcgaggcg cgcgacgcgg tcggcctgca ggcccgcgca cgcaacggcg tgccgcgcgg
    20221 cgacgtgctc gccgcccgcg acgatccgca cctcggagcc gttcgacgcg agcgtcgtga
    20281 ccttcgagcg gaagcgcacg tcgacgccct tcttcgtgag ctcggccgcc agcgtcctgg
    20341 cgacgacgcc gtagtcggtg atgccggtcg gcgggacgtg aagggccttc acgcccgcgg
    20401 cctcgggctc gatcttccgg aactcggcgg gctccatctc gcggacgccc gcgacgccgt
    20461 tctcgcggcc gcggcgggcg agctcgtcga ggcgcggcag ctcctccgcg ctcgtcgcga
    20521 ccacgagctt gccgcagatc tcgtgcggga cgccgtacgt ccggcagaag tcgacgagca
    20581 ggcgcccgcc ctcgacgcac gtcttcgcct tctccgagcc gggcttgtag tacaggcccg
    20641 agtggatgac gccgctgttg tggccggtct gatgggcgcc ggcctcgccc tccttctcga
    20701 ggaggacgac ggagagccgc gggtccttct cgagcaggcg ccacgcggtc gcgaggccca
    20761 cgatgccgcc tccgacgacg gccacgtcgt acgtcttcat gccgttcccc tcagcgcagg
    20821 agcccccgct cgtggaacga gacccaccgg ccgcgcccca cgatcacgtg gtccttcacc
    20881 tcgatgccga tctcgcggcc cgccgcgagg aagcgcttcg tcgtcgcgag gtcctccggc
    20941 gaaggctcgg gatctcccga gggatggttg tgatacagga tgacgcccgc cgcgccggcg
    21001 aggatcgcct ggcggaagag cggcgcgggc gcgacggccg cgtgcgtcac ggtgccgcgg
    21061 aagagcgccg cgtccttcac gaggcggttc ttcgcgtcga ggaggagccc gcccatgacc
    21121 tcctgcgtct cgctcgcgag gcgttccatg aggtaacgcc ccacggttgc agggtccgaa
    21181 agaagatttt tcttcgaaag tgaatcgagc gagagccttc gcccgatctc gagggccgcc
    21241 tcgattcggg tggcctttgc gggacccaca cccgaggcga gtttctcgag gtcgcgcgcc
    21301 ccgcgcgcga agagtcccgg aaacccgccg tccttcagga ggtcgcgcgc ggtctcgagc
    21361 acgggcctcg cggcggttcc cgtgccgaga aggatcgcca gaagctcttc gtcgtccaga
    21421 gcctctggac cctttctaag catcttctcc ctgggtcttt cctccttgga cctctccgcg
    21481 aggcggccgc ctcgcggccc gcgccccgcg ctcacgccgc cccccgctcc agcgcgtccg
    21541 ccagcaccgg ca
//
LOCUS       DAOMFY010000036        21535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_771_length_21535_cov_8.590829, whole genome shotgun sequence.
ACCESSION   DAOMFY010000036 DAOMFY010000000
VERSION     DAOMFY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21535
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_771_length_21535_cov_8.590829"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLB01_05030"
     CDS             complement(<1..403)
                     /locus_tag="PLB01_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76696.1"
                     /translation="MASRPLGVVIVDDEELARELVREFLAPHADVAVLATCANGFEAV
                     KAVAEKKLGAASARKLRVRMSDMAAAGRVTHLKAQFQHLPRDPIPRFDRLIGIGVGPH
                     GDRGDLVARFGQRLAQQPSRLGLGEQLRFEIQ"
     gene            complement(450..2498)
                     /locus_tag="PLB01_05035"
     CDS             complement(450..2498)
                     /locus_tag="PLB01_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQQ76697.1"
                     /translation="MLKLLLAFVSAHAFLALVSLVVILGALGAALLFRWIPNNRVGIV
                     EKLVSAKGSVASGLIALHGEAGFQPGLLRGGWHALPRFWYRVHRVPLVTIPQGKIGYV
                     FARDGQPLSPTQTLASAGKAPDYQDAAAFLAGGGQRGPQRMVLREGTWAINLALFAVV
                     TEAGVLQLALDRQDEEVLRRMAQLIAERQGFTPVVIKGTDDLVGVVTVHDGPSLAEGE
                     IIAPVVGDDPAQRATYHNKFQDPEKFLAAGGLRGRQLEVLVEGTYILNRLFATVEMIP
                     KTVVDVGSVGVVVSYTGRAGVDLSGPEYAHGETVTTGCRGVWSVPLGPGKYAFNTYAG
                     RVVTVPTTNIILKWIRSETGSHRLDENLSEIGLITKDAFEPTLPLSVVIHIDYRKAPL
                     VIQRFGDIKKLVEQTLDPMVSAYFKNVGQTRTLLQLIQDRSAIQDAASREMKEKFLRY
                     NLELEEVLIGTPGAAPGGSIEQILNQLRARQVAAEQVETYTRQQTAAVKERELREAEA
                     RAKQQSVLTESEVSITVQSNQGKAEYQRSLQQAAQIQALADAEGKKVRILADADAARI
                     QAVGGAEADRAARVGVAQAVAAEEQVRAYGGPRYQVAQAVLSRFAEAIEKSKVDIVPR
                     VMVGGGGADGSKTGNALEALFALFLANQADGGAAGAAARPEVEAMKENVRKAVFGKMG
                     "
     gene            complement(2767..3822)
                     /locus_tag="PLB01_05040"
     CDS             complement(2767..3822)
                     /locus_tag="PLB01_05040"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQQ76698.1"
                     /translation="MHPLLARSGAFAVYLIAWVPLTAILGVLLALAGGMTALEAAVVA
                     PLLTGVYAFVCLSSWYLCVAFPIRTSRLLPLAVTITVASLVAASLWVLWGATLGGLLA
                     LAPPFETIPEALRRSTPVVLLLGILLYLLAVALHYLLAAARDAKEREEREAELKSLAR
                     EAELQALKERLNPHFLFNALNSIGSLAATRPEEARRMCALLSDFLRTTLGLSGKAEIP
                     LSEEIALARRYLAVERVRFGDRLGFTEDLDPAAADSLVPPLLLQPLVENAVKHGIAGR
                     VDGGTLTLVTKRFGDRVSIVVENPAGEDAPRAAGTGLGLANVSRRVAASWGDSGHFSA
                     RRVDAMFRAEIELPARL"
     gene            complement(3832..4536)
                     /locus_tag="PLB01_05045"
     CDS             complement(3832..4536)
                     /locus_tag="PLB01_05045"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HQQ76699.1"
                     /translation="MDPTLPTPRYGSGGRLLVGLLFIAGGILFTLANFGVVHVRDVTR
                     FWPLILVAVGLLRLASRSVFAGLILVGLGVLFQLRELDLIWFRLSMLLPLIFVIVGLR
                     IATEGFRRRWPPPQNQAGAGGVVNEWAVFGGGDRRISSEFAGGQANAVFGGFNLDLRK
                     STMVGDAATIDVFCFCGGGEIRVPEGWNVVLKVVAIFGGTDDKTRHELSPAGAAPPRT
                     LYVTGFIVFGGLGVKN"
     gene            complement(4626..5753)
                     /locus_tag="PLB01_05050"
     CDS             complement(4626..5753)
                     /locus_tag="PLB01_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HQQ76700.1"
                     /translation="MRGLLRALAALAIAVFLLALFVKKRYGGPTSPFPDVSTPPVLPA
                     SAVEKVAELAEAPGNIAVSKDGRVFFNFHPEGRPKIAVAELVGGKPVPFPDASFDLWD
                     FVFSVRIDAQGLLWTLGTGFHGLRHPRLLAFDLGTRKLVHRWDLPRDVAGPGSYVQDF
                     QVSPDGKTVYLADTAAFSKRPALIVYEVGTGIGRRVLERDASVTARPYLIDAKGTKML
                     LLGGLYAMHPDVDSIALDDDGTWLYYGPMSHETLFRVRTADLRDASLPPAALSARVEV
                     FGPKPQTDGASMDRSGNVYLTDVEHGAIARLSPDRTLVTLVRDPRWRWPDGLSFGPED
                     WLYVTDSAIPDILMRTPGHVRKSAPFFIWRFRTGVPGTPGR"
     gene            complement(5760..9458)
                     /gene="metH"
                     /locus_tag="PLB01_05055"
     CDS             complement(5760..9458)
                     /gene="metH"
                     /locus_tag="PLB01_05055"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQQ76701.1"
                     /translation="MSAEARLRALLARRVAVIDGAMGTMIQREGLDEAAYRGERFRDH
                     AKDLKGANDLLVLTQPGIVEKIHRQYLEAGADIVETNTFNAQAISLADYGLEPLAYEL
                     NVAAARLARSAVDAHRVATGKDAFVAGALGPTNRTLSLAVDVNDPGKRTHVFADMVAA
                     YREQVRGHLDGGVDALLFETVFDTLVLKAALFAAQQLFDEGARRVPLMVSVTITDKSG
                     RTLSGQMVEAFWNSIAHVPLLSVGINCALGAKEMRPYVEELSGLAPVFTSAYPNAGLP
                     NAFGGFDETPAIMCADLAGFAREGWVNVVGGCCGTTPEHIRAIADAVHGIPPRRVPEI
                     PRATRLSGLEPYTLRDGVFTVVGERTNVTGSPKFAKLVLAGDYEGALAVARQQVEGGA
                     NVLDVCMDEGMLDGVAAMTRFLNLVSAEPDIAKLPIMLDSSKWDVIEAGLRCLQGKAV
                     VNSISLKEGEDAFRAHARLVRRYGAAVIVMAFDEDGQATTVERKLAIARRAYRLLAEE
                     VGFPPEDVIFDPNVLTVATGIEEHDSYAVDFFEATRRIKEALPHSKVSGGISNVSFSF
                     RGNNTVREAMHAAFLYHAIRAGLDMGIVNAGQLAVYEDVPKDLLERVEDVLLNRRPDA
                     TERLLAFAESVKQAGPAEAKADAWRSGTVGERLTHALVHGITDFVETDTEEARKALAR
                     PLDVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKRAVAYLEPYLQADKAAGAST
                     SQGRVLLATVKGDVHDIGKNIVGVVLACNGYEVVDLGVMVPADRILKAAKERSADVVG
                     LSGLITPSLEEMAHVAREMERAGFTVPLLIGGATTSPVHTAVKIAPAYGRPVVHVHDA
                     SRAVGVVSGLLGAEKAVFAETNRKKQALLAGEHEARAKKPLVPLAAARARRPKIDWAA
                     ADLPVPSFTGVRVLHDVPLETLVPFIDWSPFFHAWEMRGRHPQILDDAVLGPRARDLF
                     ADAQALLARIVKERLLTARGVHAFFPAASEGDDVAVFADEARTKRLATLHTLRQQMEK
                     PEGQPLEALADFVAPAASGRKDHIGLFAVTAGIGADALARSFEAAHDDYSAILVKALA
                     DRLAEAFAEMLHQRARADWGYGRNEALSHEELLKERYRGIRPAPGYPACPDHTEKRTL
                     FGLLGAEESAGIRLTESFAMTPAASVSGFYFAHPEAHYFAVGRIGKDQVEDYARRKGI
                     SVEEAERWLAPNLGYEPARAAIPAAAAR"
     gene            complement(9455..11299)
                     /locus_tag="PLB01_05060"
     CDS             complement(9455..11299)
                     /locus_tag="PLB01_05060"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HQQ76702.1"
                     /translation="MKKDIRERLKTEVLLGDGAMGTLLVSRGAPADGPRSPVCLTTPD
                     LVTEIHDDYVSAGAQIVSTNTWDANRSKLTKFDWADSLEKINRAAVRLAHASAEGEYV
                     YVGGVVGPLGQLVKPYGPLTRPHVREIFTEQLRILLEEKVDLLVFETFSSLLEILEAV
                     RAARALSAEIPVVASMTFLADGKTSFGDEAAESLRALADAGADVVGLNCAIGPQESLD
                     ILTRATSSVSRPVSLMPNAGYPWIVGGRTVYPATADYFRETAREAVQAGAAYLGGCCG
                     TGPEHVAAMARETVGKARRAAHAAPSVFVSSERARPEAAPLETSAFKRKLADPAARVV
                     TVEVEPPKGFDSSVALDGARTLRTLGVDAVNVTDNPMARLRMSSIAVAHLIRHETGAE
                     TVFHFSPRDRNVLGIQSDLLGAAGLGIKALLVVGGDPLKIGDYPQAKPVGEVDTLGLL
                     RIVKGLNAGVDLAGGAIGAATSFAIGCAANPAARDLDVEISKLAAKIDAGATFAQTQP
                     VWDLAALDRFCARAESRAIPVLVGLIPPKSLKQALYFANEVPGMVVPESLIERMRKAA
                     DKGAEFEEEEGIALALELAKEMAARVRGLHVMPMGRYGVVKRLMEALG"
     regulatory      complement(11355..11481)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            11562..12872
                     /gene="purD"
                     /locus_tag="PLB01_05065"
     CDS             11562..12872
                     /gene="purD"
                     /locus_tag="PLB01_05065"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQQ76703.1"
                     /translation="MRLLVVGSGGREHALVWKLAQSPGVTDIFCAPGNPGIAKLATCI
                     PISVDNVVELADFAESMKIDLTIVGPELPLTLGLVDELTHRGLLAFGPNRLAAELEGS
                     KAFSKTFMKKYAIPTADAEIVTNRAEAERALKSFGLPVVFKADGLAAGKGVVIAQEPA
                     EAEQALKLFFEDRVFGSAGDRVLVERFIEGDEVSFMVLCDGERALPLATVKDYKKIYD
                     GDRGPNTGGMGSHSPSVLLDASTASDILAFVVQPTLKGMKDEGRPFRGVLFVGVILEK
                     GLNPYVLEYNVRFGDPETQAVMLRMDGDLLPFLFASARGKFEEGATPAWRREATACLV
                     LTAEGYPGPYEKGRPITGIEEAEKEEGVFVFHGGTATTPDGQLVTAGGRVLNVCARSR
                     GLSQALKKAREACEKIHFDGRHFRRDIGKKAMEILMERSGSLLR"
     gene            complement(12869..13099)
                     /locus_tag="PLB01_05070"
     CDS             complement(12869..13099)
                     /locus_tag="PLB01_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76704.1"
                     /translation="MCLIAAELEGRGIATTSVTALPEVTRKVGIPRALAVPYALGYPL
                     GLPHDAVLQTKILVSAFGLLRKAGPPPVVRDF"
     gene            complement(13118..13615)
                     /locus_tag="PLB01_05075"
                     /pseudo
     CDS             complement(13118..13615)
                     /locus_tag="PLB01_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
     gene            13657..15225
                     /locus_tag="PLB01_05080"
     CDS             13657..15225
                     /locus_tag="PLB01_05080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ76705.1"
                     /translation="MTRIESGRSGPAWRLIGAAALVQAALHAASVGRYGIFRDELYYL
                     SCASRPAAGYVDQPPFSILFLSAWRFLFGDSAASLMVPPALCGVAVVVLTGLLARRFG
                     GGPAAQGLASAAAAVAPIWVGMTGYYSMNSFDILLWTLAATVLAALLGDEPGPRAGRL
                     WLTLGLTLALGLLNKISVLWLGAGIFAGLLLTPARRWLTTLGPWLAGGIAFLGLLPYL
                     AWNALHRWPTLEFMRRATGEKYRATSPLAFGGELLLLFQPLAAPLWIAGLVWLLAARD
                     GRRFRLLGVVFVTTFAILLANRTSKAEYLAAAFAPLLAAGGVALERLLHRGRSAAGAW
                     VYGTLLVLGGVAIAPFALPLLPVPEYLAYAKKLGMGPTTTERNELGPLPQHFADRFGW
                     PEMAAEVARVASMLTAGERGTARVWARNYGEAAALEKYGAPLGVPRVLCPHNSFWFWS
                     VADAEKNAPFKGPLIVIGGTRPDLETVFGRVEEAGKTGHPLAMPYENGRPIWICRDPK
                     EDLRAILQRDRLFI"
     gene            complement(15218..16825)
                     /locus_tag="PLB01_05085"
     CDS             complement(15218..16825)
                     /locus_tag="PLB01_05085"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQQ76706.1"
                     /translation="MSAHDWPRVQALSLHVPALGLLVDLSKTGLADDALRKHPLVTGA
                     FPAAFAAMDALEKGAIANPDEKRRVGHYWLRAPERAPDAETAEAIRGTLARIEDFAAA
                     VHAGRIAPEKAEHFTNLLVVGIGGSALGPQFVADALGSPRDPLKPFFFDNTDPDGMAR
                     EISRIAATEGGLPATLTVVISKSGGTKETRNGMLVAADAYRRAGLDFAKHAVAVTGAH
                     SELDETAAQEGWLERFPMWDWVGGRTSELSAVGLLPAALQGLDTNAMLSGAAACDAAT
                     RVPDVAQNPAAMLALAWHHETGGRGAKDMVVLPYKDRLLLFSRYLQQLVMESLGKEKD
                     LTGRVVNQGIAVYGNKGSTDQHAYVQQLREGVPNFFATFLAVEKDRADGERSLEVEPD
                     VTPGDYLHGFLLGTRKALAENGRGAITLTVPDVSARTIGILIALYERAVGLYAQLVGI
                     NAYHQPGVEAGKKAAIAVLALQARILADLRARKGEFRTAEKIAGSLSEPDVETVFAVL
                     RHLAANPDHGVAKKDGPTFFLATFGIR"
     gene            16887..17858
                     /locus_tag="PLB01_05090"
     CDS             16887..17858
                     /locus_tag="PLB01_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQQ76707.1"
                     /translation="MAKTNGKGLALGIDLGGTKILAGIVDPSNRILGRGKLKTPFADG
                     PDAIGAALVEACDLALKEAGAARGDVSSFAIAAPGAVDTIKGTLLKAANLNASNWNVV
                     KAVGSAFPGAAGRVENDVRLAALAEARLGAGRGSNLMVAVWVGTGVGGAVIMNGKIHS
                     GHNRNAGEIGHTQIDFRRAAPGTIDGTLEGTAAKVGIAAYLKRRIDRGDKTELEKAVS
                     KKDVRLKGSQLRDAWEEGDELARRALQRSARAVGITIANVWNILSPDVFVLGGGVATD
                     VGPAYLAEVRKWADAFAFTQDLGTLRVEPAGLGDDSGLLGAALYSRD"
     gene            17894..18682
                     /gene="ricT"
                     /locus_tag="PLB01_05095"
     CDS             17894..18682
                     /gene="ricT"
                     /locus_tag="PLB01_05095"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQQ76708.1"
                     /translation="MSCTGCSLGTSTNPGGNYVGARLADAEDGKTTLVFTGEVQVKPG
                     ERVIVETENGPEFAEVTTTSPTLARSCSAKKARRFLRLASDSEYADYAERLELQQAAL
                     AFADEKVAAMRLDIHVVKCAVGFDRRKLTVLYTSEKKPEVRDLSRAISDKFDLRVDMK
                     MIGVRDETKLLGGIGSCGLTLCCSTWLKGFHPVAIRMAKMQGLTPNPAKLTGQCGRLK
                     CCVAYELEGGIEAARRMGHGGDRDRREKNVPAPPTPAAPPPPSA"
     gene            18773..19210
                     /locus_tag="PLB01_05100"
     CDS             18773..19210
                     /locus_tag="PLB01_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HQQ76709.1"
                     /translation="MASAKRPVPGAPAPGEGGGPPEELGPRKLIRPSLAGVMRDQGSP
                     RPAGGGARRVVPPEQTNAEAYYYLKQMQSKTPIVVRLVDGEELRGWIEWYDKDVIKLN
                     RDHAPNLLIPKRSIKYLYKEEEERMHKKRRRPLPGAGPGVDGA"
     gene            19266..20672
                     /locus_tag="PLB01_05105"
     CDS             19266..20672
                     /locus_tag="PLB01_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ76710.1"
                     /translation="MNDSKLTKEALTAFAAQNRDRFESLLKDFVERPTVSVDPSKKPA
                     IAECVTLACDTIKAFGGVPSVLETSGHPIVHGVFGDDPSRPTVTVYNHLDVQPASKQT
                     EPWETEPFVFTKKGDRYFGRGTTDDKGPALSALFGAKAAMDAGVPVNVRVLWEFEEEI
                     GSPSFAKGIGAAKDRLATDSIIVSDTIWIARGKPAAPAGLRGLQGMLFTLETGTTDQH
                     SGVTGGAARNPMAELMQVVSECFDAKTGKVKIPGFYKDVVPPTKAELEDFRKSGFTVA
                     GFKKDHLFKSIRTNDALDVMKRIWAQPTFEIHGCVGGYTGPGVKTVIPPRAEVKVSCR
                     LVPNQTGAKIAKLVTDFVKKKNKDVKVHLEGRLDPYQGVTTGPLADAVRSSVKFAFGR
                     TPVFVREGGSIGAVLTMEQTLKAPVLFLGLSLPEHGYHAPNENYDWEQASGGMIAFAK
                     YFQEVGALGKVGAKGYRP"
     gene            complement(20725..>21535)
                     /locus_tag="PLB01_05110"
     CDS             complement(20725..>21535)
                     /locus_tag="PLB01_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76711.1"
                     /translation="PERLGSAPAGLVMTPVEFEDGRLLVPGLVAGRRQILVTGEGGDL
                     RPFTGLSEPTFGPVTLAGRDAVAFLTGSSSAPPLLAVAARADGRLVKKHALPKGAIPS
                     SLAASRDGRTIFFADGGTIWSVDAASGASKAFCAGHGVAGDPGADDVLVQRNAASGVQ
                     LVRVPFATRAETPVLTAGPLRLATAPLSGGAVGPDGRILVSAVSPETWLPVPAVLDPA
                     TGALTLIPVRAPGEILTASWGRGGTVLAMALGTNGEFWSFRPREESPGTGR"
BASE COUNT         3178 a         7537 c         7809 g         3011 t
ORIGIN      
        1 gctggatttc gaaacgaagc tgttcgccaa gccccaggcg gctcggctgt tgcgcgagac
       61 gctggccaaa ccgggctaca agatcgcccc gatcgccatg gggaccaaca ccgatcccta
      121 tcagccgatc gaagcgcggt atcggatcac gcggcaagtg ctggaactgt gccttgaggt
      181 gcgtcacccg gccggcagcg gccatatcgc tcatgcggac acgcaactta cgtgcagaag
      241 ccgccccgag cttcttctcg gcgacggcct tcacggcctc gaagccgttc gcgcacgtcg
      301 cgaggacggc gacgtcggcg tggggggcga ggaactcccg gacgagctcg cgggcgagct
      361 cctcgtcgtc cacgatcacg acaccgagcg ggcgggaggc cacggagcga ggatagctcc
      421 ggggcctccc gccgggagag gaggaagcct cagcccatct tcccgaacac ggccttgcgg
      481 acgttctcct tcatggcctc gacctccggc cgcgccgcgg cgccggcggc cccgccgtcg
      541 gcctggttcg cgagaaagag cgcgaagaga gcctcgaggg cgttgcccgt cttcgagccg
      601 tcggccccgc cgccgcccac catcacgcgc gggacgatgt cgaccttcga cttctcgatc
      661 gcctcggcga accgggagag gacggcctgc gcgacctggt agcgcggccc gccgtaggcg
      721 cggacctgct cctcggccgc gacggcctgg gcgacgccga cgcgcgccgc gcggtcggcc
      781 tcggcgccgc cgacggcctg gatgcgcgcg gcgtccgcgt ccgcgaggat gcgcactttc
      841 ttgccttccg cgtccgcgag cgcctggatc tgcgccgcct gctggagcga gcgctggtat
      901 tcggccttgc cctggttgga ctggaccgtg atcgagacct ccgactcggt caggacggac
      961 tgctgcttcg cgcgcgcctc ggcttcgcgg agctcgcgct ccttgacggc ggccgtctgc
     1021 tggcgcgtgt acgtctcgac ctgctcggcg gccacctgcc gcgcgcggag ctggttcagg
     1081 atctgctcga tcgagcctcc cggcgccgcg cccggcgtcc cgatgaggac ctcctcgagc
     1141 tcgaggttgt aacgcaggaa cttctccttc atctcgcggg aggccgcgtc ctggatcgcg
     1201 ctgcggtcct ggatgagctg gaggagcgtg cgcgtctggc cgacgttctt gaagtaggcc
     1261 gagaccatcg ggtcgagcgt ctgctcgacg agctttttga tgtcgccgaa gcgctggatg
     1321 acgagcggcg ccttgcggta gtcgatgtgg atcaccaccg agagaggcag ggtcggctcg
     1381 aacgcgtcct tcgtgatgag tccgatctcg gagaggttct cgtcgaggcg gtgcgagccc
     1441 gtctcgctcc ggatccactt caggatgatg ttcgtcgtcg gcacggtcac gacgcggccc
     1501 gcgtacgtgt tgaacgcgta cttgcccggc ccgagcggga cgctccagac gccgcggcag
     1561 cccgtggtca cggtctcgcc gtgcgcgtac tcggggcccg agaggtcgac gcccgcgcgg
     1621 cccgtgtagg agacgacgac gccgaccgag cccacgtcca cgacggtctt cgggatcatc
     1681 tcgaccgtcg cgaagaggcg gttcaggatg tacgtgccct ccacgagcac ctcgagctga
     1741 cggccgcgca ggccgccggc cgcgaggaac ttctccggat cctgaaactt gttgtggtac
     1801 gtcgcgcgct gggcggggtc gtcgcccacg acgggcgcga tgatctcgcc ctccgcgagc
     1861 gacgggccgt cgtgaacggt cacgaccccg acgaggtcgt cggttccctt gatgacgacg
     1921 ggcgtgaagc cctggcgctc ggcgatgagc tgcgccatcc ggcggaggac ctcctcatcc
     1981 tgcctgtcga gagccagctg gaggacgccg gcctccgtca cgacggcgaa gagcgcgagg
     2041 ttgatcgccc acgtgccctc gcgcaagacc atccgctgcg gcccgcgctg gccgccgccg
     2101 gcgaggaacg cggccgcgtc ctggtagtcg ggggccttgc ccgccgaggc gagcgtctgc
     2161 gtcggcgaga gcggctggcc gtcgcgcgcg aagacgtagc cgatctttcc ctgcgggatc
     2221 gtcacgagcg gcacgcggtg gactcggtac cagaagcgcg gcagggcgtg ccagccgccg
     2281 cgcaggaggc cgggctggaa gcctgcctca ccatggagcg cgatgaggcc ggacgcgacc
     2341 gaccccttcg cgctgacgag cttctccacg atcccgacgc ggttgttcgg gatccagcga
     2401 aagaggagcg ccgcccccag cgcgccgagg atgacgacga ggctcacgag ggcgaggaac
     2461 gcgtgagccg acacgaaggc caggagcagc ttcaacacgg gagctccttc cgggatcgcg
     2521 cgggtgacgg gccgctcagg cggcggagcc gcgccgcgaa tcccttctca gacggtgtgg
     2581 gaaacgcccc tgcggggagc cgtcaggccc tggggcgttt gctgcgtgac atgtttgcca
     2641 cctgatccca atgtgcgtcc ggaaccgcgc gccgtcaaat gcctctccgg cacgcgcgat
     2701 gcacgccgca cttgtacgcg gaatcacaag tgccagcttc cggcacagga atcgcgcggc
     2761 ggcgcgtcag aggcgcgcgg gcagctcgat ctcggcgcgg aacatcgcgt ccacgcggcg
     2821 cgcggagaaa tggcccgagt ccccccagga cgccgcgacg cgccgggaga cgttcgcgag
     2881 accgaggccg gttcccgcgg cgcgcggcgc gtcctcgccg gcggggttct cgacgacgat
     2941 cgacacgcgg tcgccgaagc gcttcgtgac gagcgtgagc gtcccgccgt cgacccggcc
     3001 cgcgatgccg tgcttgacgg cgttctcgac gagcggctgc aggagaagcg gcggcacgag
     3061 ggagtccgcc gcggccggat cgagatcctc ggtgaagccg aggcggtcgc cgaaccgcac
     3121 gcgctcgacg gcgaggtagc gccgcgcgag cgcgatctcc tcggacaacg ggatctcggc
     3181 cttgcccgag aggccgagcg tcgtgcgcag gaagtccgag aggagtgcgc acatgcggcg
     3241 cgcctcctcg gggcgcgtcg cagcgagcga gccgatcgag ttgagcgcgt tgaagaggaa
     3301 gtgagggttc aggcgctcct tgagcgcctg gagctcggcc tcgcgggcga gggacttgag
     3361 ctccgcctcg cgctcctcgc gttctttcgc gtcgcgcgcg gccgcgagga ggtagtggag
     3421 cgcgacggcg aggaggtaca ggaggatgcc gaggagaagg acgaccggcg tcgagcggcg
     3481 gagcgcctcg gggatcgtct cgaacggcgg cgcgagcgcg aggaggccgc cgagcgtcgc
     3541 gccccagagc acccagagcg acgcggcgac gagggacgcg acggtgatcg tgacggcgag
     3601 cgggaggagc cgggaggtcc ggatcgggaa cgcgacgcag agataccacg aggagaggca
     3661 gacgaaggca tagacgcccg tcaggagcgg cgcgacgacg gcggcctcga gagccgtcat
     3721 cccgcccgcg agtgcgagga gaacgccgag gatggccgtg agcgggaccc acgcgatcag
     3781 gtagaccgcg aaggcgcccg accgcgcgag aagcggatgc acggcgcgcc ttcagttctt
     3841 gacgccgaga ccgccgaaga cgatgaatcc cgtgacgtag agggttctcg ggggtgcggc
     3901 gccggccgga gacagttcgt ggcgcgtctt gtcgtccgtg ccgccgaaga tcgcgacgac
     3961 cttcaggacg acgttccagc cttccggcac gcggatctcg ccgccgccgc agaagcagaa
     4021 gacgtcgatc gtggccgcgt cgcccaccat cgtggacttc ctcaggtcga ggttgaatcc
     4081 gccgaagacg gcgttcgcct gcccgcccgc gaactcggag gagatcctcc ggtcgccgcc
     4141 gccgaagacg gcccactcgt tcacgacgcc gcccgccccg gcctggttct gcggcggcgg
     4201 ccagcggcgc cggaacccct ccgtcgcgat cctcaggccg acgatcacga agatgagcgg
     4261 caggagcatc gagagccgga accagatgag gtcgagctcg cggagctgga agaggacgcc
     4321 gagacccacg aggatgaggc cggcgaagac gctccgcgac gcgaggcgga ggaggcccac
     4381 cgcgacgagg atgagcggcc agaacctcgt gacgtcgcgg acgtggacga cgccaaagtt
     4441 cgcgagcgtg aagaggatgc cgcccgcgat gaagagaagc ccgacgagca ggcgtccgcc
     4501 tgagccgtat cggggggtcg gaagggtcgg gtccatggct gcctccacgg gaaaggtacg
     4561 cgcccgaggg gactccggcc cgtgaaaatc ggccaacgaa cggccgggat cggcgaacgg
     4621 gagcctcagc ggccgggagt gccggggacg ccggtccgga agcgccagat gaagaacggg
     4681 gcgctcttcc tcacgtgtcc gggcgtccgc atgaggatgt ccgggatcgc gctgtccgtc
     4741 acgtagagcc agtcctccgg gccgaaggag agcccgtcgg gccagcgcca ccgagggtcc
     4801 cgaacgagcg tcacgagcgt gcggtccggc gagagccggg cgatcgcgcc gtgctcgacg
     4861 tcggtcaggt agacgttccc gctccggtcc atgctcgcgc cgtccgtctg gggcttcgga
     4921 ccgaagacct cgactctcgc ggagagcgcg gccgggggga gcgaggcgtc ccgcaggtcg
     4981 gccgtccgga cgcggaagag cgtctcgtgc gacatcggcc cgtagtacag ccacgttccg
     5041 tcgtcgtcga gcgcgatcga gtcgacgtcc gggtgcatcg cgtagaggcc cccgaggagg
     5101 agcatcttcg ttcccttcgc gtcgatgagg tacgggcgcg ccgtcaccga agcgtcgcgc
     5161 tcgagaacgc gccgcccgat tcccgtaccg acctcgtaga cgatgagggc gggcctcttc
     5221 gagaacgcgg ccgtgtccgc gaggtagacc gtcttcccgt ccgggctgac ctgaaagtcc
     5281 tgcacgtacg agccgggccc cgcgacgtcg cgcggcagat cccagcggtg aacgagcttg
     5341 cgggtcccga gatcgaaggc gaggaggcgc gggtggcgaa ggccgtggaa accggtcccg
     5401 agcgtccaga gaagcccctg ggcgtcgatc ctcacggaga agacgaagtc ccacagatcg
     5461 aaggaggcgt ccggaaaggg aaccggcttt ccgccgacga gctcggcgac ggcgatcttc
     5521 ggacgtccct cggggtgaaa gttgaagaag acgcgcccgt ccttcgaaac ggcgatgttg
     5581 ccgggagcct cggccagctc ggcgaccttc tcgacggccg aggcgggcag gacaggcggc
     5641 gtcgacacgt ccgggaaggg ggacgtcggc ccgccgtagc gcttcttgac gaacaacgcg
     5701 agcaggaaga cggcaatcgc gagagcggcg agggcgcgaa ggaggcctct catgcgcgtt
     5761 cagcgggccg cggcggcggg gatggcggcc cgcgcgggct cgtacccgag gttcggcgcg
     5821 agccagcgct cggcctcctc gacggagatc cccttccggc gcgcgtagtc ctcgacctgg
     5881 tccttcccga tccggccgac cgcgaagtag tgggcctcgg ggtgtgcgaa gtagaagccc
     5941 gaaaccgacg cagcgggcgt catcgcgaag ctctcggtta ggcggatgcc ggcggattcc
     6001 tccgcgccga ggaggccgaa gagcgtgcgc ttctcggtgt ggtccgggca ggcgggatag
     6061 cccggcgccg ggcggatgcc gcggtacctc tccttgagaa gctcctcgtg cgaaagggcc
     6121 tcgttcctcc cgtagcccca gtccgcgcgg gcgcgctggt gcagcatctc ggcgaaggcc
     6181 tcggcgaggc ggtccgcgag cgccttcacg aggatggcgg agtagtcgtc gtgcgcggcc
     6241 tcgaaggagc gtgcgagcgc gtccgcgccg atgccggcgg tcacggcgaa gaggccgatg
     6301 tggtccttcc gtccgctcgc ggcgggcgcg acgaagtccg cgagcgcctc gagcggctgg
     6361 ccctcgggct tctccatctg ctgccgcagc gtgtggagcg tcgcgagcct cttcgtccgc
     6421 gcctcgtcgg caaagacggc gacgtcgtcg ccctcggacg cggcggggaa gaacgcgtgg
     6481 acgccgcggg cggtcaggag ccgctccttc acgatccgcg cgagcagcgc ctgcgcgtcc
     6541 gcgaagaggt cgcgggcccg cgggccgagg acggcgtcgt cgaggatctg cgggtggcgc
     6601 ccgcgcatct cccacgcgtg gaagaacggc gaccagtcga tgaacgggac gagcgtctcg
     6661 agcggcacgt cgtggaggac gcgcaccccg gtgaacgacg ggacggggag gtccgccgcc
     6721 gcccagtcga tcttcgggcg gcgggcgcgc gccgccgcga gcgggacgag gggcttcttc
     6781 gcccgcgcct cgtgctcgcc ggcgaggagc gcctgcttct tccggttcgt ctcggcgaac
     6841 acggccttct ccgcgccgag caggccggag acgacgccca cggcgcgtga cgcgtcgtgc
     6901 acgtggacga cgggccgccc gtacgcgggg gcgatcttca ctgcggtgtg gacggggctc
     6961 gtcgtcgcgc cgccgatgag gagcggcacg gtgaatcccg cgcgctccat ctcgcgcgcg
     7021 acgtgcgcca tctcctcgag cgacggcgtg atgaggcccg agaggccgac gacgtcggca
     7081 gagcgctcct tcgcggcctt caggatgcgg tccgccggca ccatgacgcc gaggtcgacc
     7141 acctcgtagc cgttgcacgc gaggacgacg ccgacgatgt tcttcccgat gtcgtggacg
     7201 tcgcccttga cggtcgcgag gaggacccgg ccctgcgacg tcgaggcccc ggcggccttg
     7261 tcggcctgga ggtacggctc gaggtacgcc acggcgcgct tcatgacgcg ggcgctcttc
     7321 acgacctgcg gaaggaacat cttcccggct ccgaagaggt cgccgacgac gttcatgccg
     7381 gccatgagcg gcccttcgat cacgtccagc ggccgggcga gcgccttgcg cgcctcctcg
     7441 gtgtccgtct ccacgaagtc cgtgatgccg tggacgagcg cgtgcgtgag gcgctccccg
     7501 acggttccgc tccgccacgc gtcggctttc gcctcggccg ggccggcctg cttgacggac
     7561 tcggcgaatg cgaggagccg ctccgtcgcg tcgggtcgcc ggttcaggag gacgtcctcg
     7621 acccgttcga ggaggtcctt cgggacgtcc tcgtagacgg cgagctggcc cgcgttcacg
     7681 atccccatgt cgaggccggc ccggatcgcg tggtacagga acgccgcgtg catcgcctcg
     7741 cggacggtgt tgttgccgcg gaacgagaac gacacgttcg agattcctcc ggagaccttg
     7801 gaatgcggca aggcctcctt gatgcggcgc gtcgcctcga agaagtcgac cgcgtacgaa
     7861 tcgtgctcct cgatccccgt cgcgaccgtc aggacgttcg ggtcgaagat cacgtcctcg
     7921 ggcgggaagc cgacctcctc ggcgaggagc cggtaggcgc gccgcgcgat cgcgagcttg
     7981 cgctccacgg tcgtcgcctg gccgtcctcg tcgaacgcca tgacgatcac ggcggccccg
     8041 tagcggcgga cgagccgcgc gtgggcgcgg aacgcgtcct cgccttcctt caggctgatc
     8101 gagttcacga cggccttgcc ctgcaggcag cggagcccgg cctcgatcac gtcccacttc
     8161 gagctgtcga gcatgatcgg cagcttcgcg atgtcgggct cggccgagac gagattcaag
     8221 aagcgcgtca tcgccgcgac gccgtcgagc atcccctcgt ccatgcagac gtcgaggacg
     8281 ttcgcgccgc cctccacctg ctggcgcgcg acggcgagcg cgccctcgta gtcgcccgcg
     8341 aggacgagct tcgcgaactt cggcgagccc gtcacgttcg tgcgttcgcc gacgacggtg
     8401 aagacgccgt cgcgcagggt gtacggctcg aggccggaga ggcgcgtcgc gcgcgggatc
     8461 tccgggaccc gccgcggcgg gatgccgtgg acggcgtccg cgatcgcgcg gatgtgctcc
     8521 ggcgtcgtcc cgcagcagcc gcccacgacg ttcacccagc cctcgcgcgc gaaccccgcg
     8581 aggtcggcgc acatgatcgc gggcgtctcg tcgaagccgc caaacgcgtt cgggaggccc
     8641 gcgttcgggt acgcgctcgt gaagacgggc gcgaggcccg agagctcctc gacgtacggg
     8701 cgcatctcct tcgccccgag cgcgcagttg atcccgacgg aaaggagagg gacgtgcgcg
     8761 atcgagttcc agaacgcctc gaccatctgg cccgagagcg tccggccgga tttgtcggtg
     8821 atcgtgacgg aaaccatgag gggcacgcgc cgcgcgccct cgtcgaagag ctgctgcgcc
     8881 gcgaagaggg ccgccttgag gacgagcgtg tcgaagaccg tctcgaagag gagtgcgtcg
     8941 acgccgccgt cgaggtggcc gcgcacctgc tcccggtagg ccgcgaccat gtccgcgaag
     9001 acgtgcgtgc gcttcccggg gtcgttcacg tccacggcga gggagagcgt ccggttcgtg
     9061 ggcccgagcg cgcccgcgac gaaggcgtcc ttacctgtgg ccacgcggtg ggcgtcgacg
     9121 gcgctccgcg cgaggcgcgc cgcggcgacg ttcagctcgt acgcgagcgg ctcgagaccg
     9181 tagtcggcga gcgagatcgc ctgcgcgttg aacgtgttcg tctcgacgat gtccgcgccg
     9241 gcctcgagat actggcggtg gatcttctcg acgattccgg gctgcgtcag aacgaggagg
     9301 tcgttcgcgc ccttgaggtc cttcgcgtga tctcggaaac gctccccgcg gtacgcggct
     9361 tcgtccagcc cctcgcgctg gatcatcgtc cccatcgcgc cgtcgatcac ggcgacgcgg
     9421 cgggcgagaa gcgccctcag gcgcgcttcg gcgctcatcc gagagcctcc atgagccgct
     9481 tgacgacgcc gtagcgaccc atcggcatca cgtgcaggcc gcggacgcgc gccgccatct
     9541 ccttcgcgag ctcgagagcg agcgcgatgc cctcctcctc ctcgaactcg gcccccttgt
     9601 cggcggcctt gcgcatccgc tcgatcaggg actcggggac gaccatcccc gggacctcgt
     9661 tcgcgaagta gagggcctgc ttgaggctct tcggcggaat gagcccgacg aggacgggga
     9721 tcgcgcggga ctcggcgcgc gcgcagaacc gatccagcgc ggccaggtcc cagacgggct
     9781 gcgtctgggc aaaggtcgcc ccggcgtcga tcttcgcggc gagcttggag atctcgacgt
     9841 cgaggtcccg cgcggccggg ttcgcggcgc agccgatcgc gaacgacgtc gcggcgccga
     9901 tcgcccctcc cgcgaggtcg acgccggcgt tcaggccctt cacgatgcgc aggaggccga
     9961 gcgtgtccac ctcgccgacg ggcttggcct gcgggtagtc gccgatcttc agcgggtcgc
    10021 cgcccacgac gaggagcgcc ttgatcccga ggccggcggc cccgaggaga tccgactgga
    10081 tgccgagcac gtttcggtcg cgcggcgaga agtggaagac ggtctcggcg cccgtctcgt
    10141 ggcggatgag gtgggcgacg gcgatcgagg acatcctcag gcgcgccatc gggttgtccg
    10201 tgacgttcac ggcgtctacg ccgagcgtcc ggagcgtgcg cgcgccgtcc agcgccaccg
    10261 aggagtcgaa gcccttcggc ggctcgacct cgaccgtcac gacgcgggcc gcggggtcgg
    10321 cgagcttgcg cttgaacgcg gacgtctcga gcggggcggc ctcgggccgc gcgcgctcgg
    10381 acgagacgaa gacggacggg gccgcgtgcg cggcgcggcg ggccttgccg acggtctcgc
    10441 gcgccatcgc cgcgacgtgc tcggggccgg tgccgcagca gcctccgagg tacgcggcgc
    10501 cggcctgcac ggcctcgcgc gccgtctcgc ggaagtagtc cgcggtcgcg gggtagacgg
    10561 tgcggccgcc gacgatccac gggtagcccg cgttcggcat gagcgagacg ggacgggaga
    10621 ccgaggacgt ggcgcgcgtc aggatgtcga gggattcctg cggaccgatc gcgcagttca
    10681 ggccgacgac gtccgcgccc gcgtccgcga gcgcgcggag actctcggcc gcctcgtcgc
    10741 cgaagctcgt cttgccgtcc gcgaggaagg tcatggacgc gacgactgga atctccgccg
    10801 agagggcgcg cgcggcgcgc acggcctcga ggatctcgag gagcgacgag aacgtctcga
    10861 agacgaggag gtcgaccttc tcctcgagga ggatccggag ctgctccgtg aagatctcgc
    10921 ggacgtgcgg gcgcgtgagc gggccgtacg gcttcacgag ctggccgagc ggcccgacga
    10981 cgccgccgac gtagacgtac tcgccctcgg ccgaggcgtg cgcgaggcgg accgcggccc
    11041 ggttgatctt ctcgagcgag tccgcccagt cgaacttcgt gagcttggag cggttcgcgt
    11101 cccacgtgtt cgtcgagacg atctgcgcgc cggccgacac gtagtcgtcg tggatctccg
    11161 tgacgaggtc cggcgtcgtc aggcagaccg gcgaacgagg tccgtccgcg ggggcgccgc
    11221 gcgacacgag gagcgtgccc atggcgccgt cgccgagcag gacctcggtc ttcaggcgct
    11281 cgcggatgtc tttcttcatg aaaacgcccc ttcgaggacg aaggggcgtg ggacgttccg
    11341 ccgactcggc ctcgatctca tctgtctccg ggccggaggc ccggagcggg atttggcacc
    11401 tgcgtccggg gcgccgcggg gcgctccgga ccggttgccg cggcatcgca gggcccgtcc
    11461 ctccgccgct ctggatgaaa cccggtcaaa gaacgagcat ccagagtacg gcgcgcgccg
    11521 ggacgcgtca aggcggggcg cggtaagatt ccacccgtca catgagactt ctcgtggtcg
    11581 gctccggcgg gcgagagcac gcactcgtct ggaagctcgc ccagtccccg ggcgtcacgg
    11641 acatcttctg cgcgccgggc aaccccggca tcgcgaagct cgcgacgtgc attccgatct
    11701 ccgtcgacaa cgtcgtcgag ctggccgatt tcgccgagtc gatgaagatc gacctgacga
    11761 tcgtcgggcc cgagcttccg ctcacgctcg gcctcgtgga cgagctgacg caccgcgggc
    11821 tcctcgcctt cggccccaac cgcctcgcgg ccgagctcga gggctcgaag gcgttctcga
    11881 agacgttcat gaagaagtac gcgatcccga cggccgacgc cgagatcgtc acgaaccgcg
    11941 ccgaggccga gcgcgccctg aagtcgttcg gcctgcccgt cgtcttcaag gccgacggcc
    12001 tcgcggccgg caagggcgtc gtcatcgcgc aggagccggc cgaggccgag caggcgctca
    12061 agctcttctt cgaggaccgc gtgttcggct cggcgggcga ccgcgtcctc gtggagcggt
    12121 tcatcgaggg ggacgaggtc agcttcatgg tcctctgcga cggcgagcgc gcgctcccgc
    12181 tcgcgaccgt gaaggactac aagaagatct acgacggcga ccgcggcccg aacacgggcg
    12241 gcatgggctc gcacagtccg tccgtcctgc tcgacgcgtc caccgcctcc gacatcctcg
    12301 cgttcgtcgt ccagccgacg ctgaagggga tgaaggacga ggggcggccg ttccgcggcg
    12361 tcctcttcgt cggcgtcatc ctcgagaagg gcctcaaccc gtacgtcctc gagtacaacg
    12421 tgcgcttcgg cgatcccgag acgcaggcgg tgatgctccg tatggacggc gacctcctgc
    12481 cgttcctctt cgcctccgcc cgcgggaagt tcgaggaggg cgccacgccc gcctggcggc
    12541 gcgaggccac ggcctgcctg gtcctgacgg ccgagggcta ccccggcccg tacgagaagg
    12601 gccgcccgat cacgggcatc gaggaggccg agaaggagga gggcgtgttc gtcttccacg
    12661 gcggcaccgc gacgaccccc gacgggcagc tcgtgacggc cggcgggcgc gtcctgaacg
    12721 tctgcgcgcg ctcgcgcggg ctctcccagg cgctgaagaa agcgcgcgag gcgtgcgaga
    12781 agatccactt cgacgggcgg cacttccgcc gcgacatcgg caagaaggcc atggagatcc
    12841 tgatggagcg gtcgggctcg ctcctgcgct agaagtcgcg gacgaccggc ggagggccgg
    12901 ccttgcgcag cagcccaaag gccgacacca ggatcttcgt ctggagcacg gcgtcatggg
    12961 gcagcccgag cggatagccg agcgcgtacg gaaccgcgag cgcgcgcggg atgccgacct
    13021 tgcgcgtgac ctcgggcagc gccgtgacgg acgtcgtcgc gatccctcgt ccttccagct
    13081 ccgcggcgat cagacacacg gtctgatggc agagcggtca aagcgggacg aggagcgcga
    13141 cgtcgacgcc gtcggcctcg aggagcgcgg cggccgcggg tgcggtctcc ttcacgagcc
    13201 ggcccggcgc cgagatcgac cccatgaacg agagggcgtt cggggcgagg ctcccgatca
    13261 ggccctccgc ctccatctcg cgcagccggt cgagcgggaa gccgaggttt cggtcggcga
    13321 ggaacccggc ctggtcgaac gcatcggact tgtgcgcgac gccgagcgac ccgacgtcga
    13381 cgtcccgcgg gatcacgcgg aacgagaagt cgccgccctt catctcctcg tcgaacgccg
    13441 cctgtcccgg cgcgtagacg gcgccggtcg tcacgagcgc gacgcgcgcc tcgcggagcg
    13501 gcctccgcgg ccgtgcgaac gggatcgggt cgaccgtccg gtaccggtac gtcgccatga
    13561 agaggcggtt cctgatggag aggtccgaaa gcgtcgccac gcgacgagta tccccctccg
    13621 tgcgaaaccc gtgcgcccgc ggcgcgtagg acgctcgtga cgcggatcga gtcggggcgc
    13681 agcgggcccg cctggcggct gatcggcgcc gccgcgctcg tgcaggccgc gctccacgcc
    13741 gcgtccgtcg gccggtacgg gatcttccgc gacgagctct actacctctc gtgcgcgagc
    13801 cgcccggcgg cgggctacgt cgaccagccg ccgttctcga tcctcttcct ttccgcgtgg
    13861 cggttcctct tcggcgattc ggctgcgtcg ctgatggtcc cgcccgcgct ctgcggggtc
    13921 gccgtcgtgg tgctcacggg cctcctcgcg cggcgtttcg gcggcggccc ggcggcgcag
    13981 ggcctcgcgt ccgcggcggc ggccgtcgcg ccgatctggg tcgggatgac cgggtactac
    14041 tcgatgaact ccttcgacat cctgctctgg acgctcgcgg cgaccgtcct cgcggccctt
    14101 ctcggcgacg agcccggccc gcgcgcgggg cggctctggc tcacgctcgg tctcactctc
    14161 gcgctcggcc tcctgaacaa gatcagcgtc ctctggctcg gcgcggggat cttcgccggg
    14221 ctcctcctca cgcccgcgcg gcgctggctg acgacgctcg gcccgtggct cgcgggcgga
    14281 atcgcgttcc tcgggctcct cccgtacctc gcgtggaacg ccctccaccg ctggccgacg
    14341 ctggagttca tgcggcgtgc gacgggcgag aagtaccgcg cgacgtcgcc gctcgcgttc
    14401 ggcggagagc tcctccttct cttccagccg ctcgcggcgc cgctgtggat cgcggggctc
    14461 gtctggctgc tcgcggcgcg ggacggccgg cgcttccgcc tcctcggcgt cgtgttcgtc
    14521 acgacgttcg caatcctcct cgcgaaccgc acgagcaagg ccgagtacct cgcggcggcg
    14581 ttcgcgccgc tcctcgcggc gggcggcgtc gccctcgagc gccttctcca ccgcgggcgc
    14641 agcgcggcgg gcgcatgggt ctacgggacg ctcctcgttc tcgggggcgt cgcgatcgcg
    14701 ccgttcgcgc tgccgctcct gcccgtcccc gagtacctcg catatgcgaa gaagctcggg
    14761 atgggcccga cgacgaccga gcgcaacgag ctgggaccgc ttccccagca cttcgcggac
    14821 cgcttcggct ggccggagat ggccgccgag gtcgcgcgcg tcgcctcgat gctgacggcc
    14881 ggggagcgcg ggacggcccg cgtctgggcg cgcaactacg gcgaggcggc ggcgctggag
    14941 aagtacggcg cgccgctcgg cgtcccgcgc gtcctctgcc cgcacaacag cttctggttc
    15001 tggagcgtcg ccgacgcgga gaagaacgcg ccgttcaagg gcccgctgat cgtgatcggc
    15061 gggacgcgcc cggatctcga gacggtcttc ggccgcgtcg aggaggccgg gaagaccggc
    15121 cacccgctcg cgatgcccta cgagaacggg cggccgatct ggatctgccg cgacccgaag
    15181 gaggacctcc gggccatcct tcagcgcgac cggctgttca tctgatcccg aacgtcgcga
    15241 ggaagaacgt cgggccgtcc ttcttcgcga cgccgtggtc ggggttcgcg gcgaggtggc
    15301 ggaggacggc gaagacggtc tcgacgtccg gctcggagag cgagcctgcg atcttctcgg
    15361 cggtcctgaa ctcgcctttc ctcgccctga ggtccgcgag gatccgggcc tgcagcgcga
    15421 ggacggcgat ggcggccttc ttgcccgcct cgacgcccgg ctggtggtac gcgttgatgc
    15481 cgacgagctg tgcgtagagg ccgacggccc gctcgtagag cgcgatcagg attccgatcg
    15541 tccgcgccga cacgtccggc accgtgagcg tgatcgcccc gcggccgttc tcggcgagcg
    15601 ccttccgggt cccgaggagg aagccgtgca ggtagtcgcc gggcgtgacg tcgggctcca
    15661 cctcgagcga gcgctcgccg tccgcgcggt ccttctcgac cgcgaggaac gtcgcgaaga
    15721 agttcgggac gccctcgcgc agctgctgga cgtacgcgtg ctggtccgtc gagcccttgt
    15781 tgccgtagac cgcgatcccc tggttcacga cgcggcccgt gaggtccttc tccttgccga
    15841 gcgactccat gacgagctgc tggagatagc gcgagaagag gaggagccgg tccttgtacg
    15901 ggaggacgac catgtccttc gcgccgcggc cgcccgtctc gtgatgccac gcgagcgcga
    15961 gcatcgccgc ggggttctgc gcgacgtcgg ggacgcgcgt cgccgcgtcg caggccgccg
    16021 cgccggagag catcgcgttg gtgtcgaggc cttggagcgc cgcgggcagg aggccgacgg
    16081 ccgagagctc ggacgtccgc ccgcccaccc agtcccacat cggaaagcgc tcgagccagc
    16141 cctcctgcgc ggccgtctcg tcgagctcgc tgtgagcgcc ggtgacggcg accgcgtgct
    16201 tcgcgaagtc gaggccggcc cgccggtacg cgtccgcggc gacgagcatc ccgttgcgcg
    16261 tctccttcgt cccgcccgac ttcgagatca cgaccgtgag cgtcgcgggg aggccgccct
    16321 ccgtcgccgc gatgcgcgag atctcgcgcg ccatgccgtc ggggtccgtg ttgtcgaaga
    16381 agaacggctt cagcgggtcg cgcggcgagc ccagcgcgtc ggcgacgaac tgcggcccga
    16441 gcgccgagcc gccgatcccg acgacgagga ggttcgtgaa gtgttcggcc ttctcgggcg
    16501 caatgcgtcc cgcgtggacc gcggccgcga agtcctcgat gcgcgcgagc gtcccgcgga
    16561 tcgcctcggc ggtctccgcg tccggcgcgc gctccggggc ccgcagccag tagtggccca
    16621 cgcggcgctt ctcgtccggg ttcgcgatcg cgcccttctc gagcgcgtcc atcgcggcga
    16681 aggcggcggg gaacgcgccc gtcacgagcg ggtgcttccg cagcgcgtcg tccgcgaggc
    16741 ccgtcttcga gaggtcgacg aggaggccga gcgcgggcac gtggagcgac agggcctgga
    16801 cgcggggcca gtcgtgagcg gacatcggga gcctcccccc tccatgatct ccgatccggg
    16861 cgggggccgc gtaaactgcg cgcctcatgg cgaaaacgaa cggcaagggc ctcgccctcg
    16921 ggatcgacct cggcggcacg aagatcctcg cggggatcgt cgacccgtcg aaccgcatcc
    16981 tcgggcgcgg caagctcaag actcccttcg ccgacggccc cgacgcgatc ggcgccgcgc
    17041 tcgtcgaggc ctgcgacctc gccttgaagg aagccggcgc cgcgcgcggc gacgtctcct
    17101 ccttcgcgat cgcggcgccg ggcgccgtgg acacgatcaa ggggacgctg ctcaaggccg
    17161 cgaacctcaa cgcctcgaac tggaacgtcg tgaaggccgt cgggtcggcg ttccccggcg
    17221 cggcgggccg cgtcgagaac gacgtgcgcc tcgcggcgct cgcggaggcc cgcctcggcg
    17281 ccggccgcgg ctcgaacctc atggtcgccg tgtgggtcgg aacgggcgtc ggcggcgccg
    17341 tgatcatgaa cgggaagatc cactcggggc acaaccgcaa cgcgggggag atcgggcaca
    17401 cgcagatcga cttccgccgg gcggcccccg gcaccatcga cgggacgctc gagggcacgg
    17461 ccgccaaggt cgggatcgcg gcttacctca agcgtcggat cgaccgcggc gacaagaccg
    17521 agctcgagaa agcggtctcg aagaaggacg tccgcctcaa gggctcgcag ctgcgcgacg
    17581 cgtgggagga gggcgacgag ctcgcgcgcc gcgcgctcca gcgctcggcc cgcgccgtcg
    17641 ggatcacgat cgcgaacgtc tggaacatcc tctcgcccga cgtcttcgtc ctcgggggag
    17701 gcgtcgcgac cgacgtgggt ccggcctacc tggcggaggt ccgcaagtgg gcggacgcct
    17761 tcgcgttcac gcaggacctc ggcacgctcc gggtggagcc ggccggcctc ggggacgact
    17821 ccgggctcct cggggcggcg ctctactcgc gcgactgagt cccgcccggc tcatctggca
    17881 caatcggact cccatgagct gtacgggctg ttccctcggc acctccacga accccggcgg
    17941 gaactacgtc ggggcgcgcc tcgccgacgc cgaggacggc aagacgacgc tcgtcttcac
    18001 gggcgaggtc caggtcaagc ccggcgagcg cgtcatcgtc gagaccgaga acggccccga
    18061 gttcgcggag gtcacgacga cgtcgccgac cctcgcgcgg tcctgctcgg cgaagaaggc
    18121 gcgccggttc ctgcggctcg cctccgattc cgagtacgcc gactacgccg agcgcctcga
    18181 gctgcagcag gccgccctcg cgttcgcgga cgagaaggtc gccgcgatgc gcctcgacat
    18241 ccacgtcgtc aagtgcgccg tcggcttcga ccgccgcaag ctgaccgtcc tctacacgtc
    18301 ggagaagaag cccgaggtgc gcgacctctc gcgggcgatc tcggacaagt tcgacctccg
    18361 ggtcgacatg aagatgatcg gcgtccgcga cgagacgaag ctcctcggcg gcatcggctc
    18421 gtgcggcctg acgctgtgct gctcgacgtg gctcaagggc ttccaccccg tcgcgatccg
    18481 gatggcgaag atgcaggggc tcacgccgaa ccccgccaag ctcacgggtc agtgcggccg
    18541 cctgaagtgc tgcgtggcct acgagctcga gggcggcatc gaggcggccc gccggatggg
    18601 ccacggcggc gaccgcgatc gccgtgaaaa gaacgtcccg gctccgccga ccccggcggc
    18661 tcccccgcct ccttccgcct gaggccggtt tcgccctccg gggatgcccg gaatagactc
    18721 cgggcaacat ccgttccccc tgccgcgtag acaccccttg aggagtcccc acatggcttc
    18781 cgcgaaaagg cccgttccag gcgcccctgc ccccggcgag gggggaggtc cgcccgagga
    18841 gctgggcccg cgcaagctca tccggccctc gctggccggc gtcatgcgcg accagggctc
    18901 gccccggccg gccggcggcg gcgcccggcg cgtcgtcccc cccgagcaga cgaacgcgga
    18961 ggcctactac tacctcaagc agatgcagtc caagacgccg atcgtcgtcc gcctcgtgga
    19021 cggcgaggag ctccgcggct ggatcgagtg gtacgacaag gacgtcatca agctcaaccg
    19081 ggaccacgca ccgaacctcc tgatcccgaa gcgctccatc aagtacctct acaaggagga
    19141 agaggagcgc atgcacaaga agaggcggcg gccgctgccc ggcgcgggcc ccggggtcga
    19201 cggcgcctag atgacgtccc tgcgggattg acggcgcgcc gcgcggcggg catacactcg
    19261 cccgcatgaa cgacagcaaa ctcaccaagg aagccctcac ggcgttcgcg gcccagaacc
    19321 gcgatcgctt cgaatccctc ctcaaggact tcgtcgagcg cccgacggtc tccgtcgacc
    19381 cttcgaagaa gcccgccatc gccgagtgcg tgacgctcgc gtgcgacacg atcaaggcgt
    19441 tcggaggcgt tccgtccgtt ctcgagacga gcggccaccc gatcgtccac ggcgtgttcg
    19501 gcgacgaccc gtcccgcccg accgtcacgg tctacaacca cctcgacgtc cagcccgcct
    19561 ccaagcagac ggagccgtgg gagacggagc cgttcgtgtt cacgaagaag ggcgaccgct
    19621 acttcggccg cggcacgacg gacgacaagg gccccgcgct ctcggcgctg ttcggcgcga
    19681 aggccgcgat ggacgccggc gtcccggtga acgtccgcgt cctgtgggag ttcgaggagg
    19741 agatcggctc gccgagcttc gcgaagggca tcggcgccgc caaggacagg ctcgcgaccg
    19801 actcgatcat cgtctcggac acgatctgga tcgcccgcgg caagcccgcg gcgccggccg
    19861 gcctgcgcgg cctgcagggc atgctcttca cgctcgagac gggcacgacg gaccagcact
    19921 cgggcgtgac gggcggcgcg gcgcgcaacc cgatggccga actcatgcag gtcgtctcgg
    19981 agtgcttcga cgcgaagacg ggcaaggtca agatccccgg cttctacaag gacgtggtcc
    20041 cgccgacgaa ggccgagctc gaggacttcc ggaagtccgg cttcacggtc gccggcttca
    20101 agaaggacca cctgttcaag tcgatccgca cgaacgacgc gctggacgtc atgaagcgca
    20161 tctgggcgca gccgacgttc gagatccacg gctgcgtcgg cggctacacg ggcccgggcg
    20221 tgaagacggt catcccgccg cgcgccgagg tcaaggtctc ctgccgcctc gtcccgaacc
    20281 agacgggcgc gaagatcgcg aagctcgtga cggacttcgt gaagaagaag aacaaggacg
    20341 tgaaggtcca cctcgagggg cgcctcgacc cgtaccaggg cgtgacgacg ggtccgctcg
    20401 cggacgcggt gcgctcgagc gtgaagttcg cgttcggaag gacgcccgtc ttcgtgcgcg
    20461 agggcggctc gatcggcgcg gtgctcacga tggagcagac gctcaaggcg ccggtcctct
    20521 tcctcgggct ctctctgccc gagcacggct accacgcccc gaacgagaac tacgactggg
    20581 agcaggcctc gggcggcatg atcgcgttcg cgaagtactt ccaggaggtc ggcgcgctcg
    20641 gcaaggtcgg cgcgaagggc taccggccgt agcccatggg gggtgcgggg ggagcgggga
    20701 agctcccccc gccaagaccc tcgatcaccg tcccgttccg ggactctctt ctctcgggcg
    20761 gaagctccag aactcgccgt tcgtgccgag cgccatcgcg aggacggtgc cgcctcggcc
    20821 ccacgaggcc gtcaggatct cgcccggggc gcgcacgggg atgagcgtga gcgcgccggt
    20881 cgcggggtcg agcaccgcgg gaacgggcag ccaggtctcc ggcgacacgg ccgagacgag
    20941 gatcctcccg tcgggcccca ccgcgccgcc cgagagcgga gcggtcgcga ggcggagcgg
    21001 cccggccgtg aggacgggag tctccgcgcg cgtcgcgaac ggaacgcgga cgagctgcac
    21061 gcccgacgcc gcgttgcgct ggacgaggac gtcgtccgcg cccgggtcgc ccgcgacgcc
    21121 gtgtccggcg cagaacgcct tcgaggcgcc gctcgcggcg tccacggacc agatcgtccc
    21181 gccgtccgcg aagaagatcg tccggccgtc gcgggacgcc gcgagggacg acgggatcgc
    21241 gcccttgggc agcgcgtgct tcttgacgag gcgcccgtcg gcgcgcgccg cgaccgcgag
    21301 gagcggcggc gccgacgacg aaccggtgag gaacgcgacg gcgtcccgcc ctgcgagcgt
    21361 cacggggccg aacgtcggct ccgagagccc ggtgaagggc cggaggtcgc cgccttctcc
    21421 cgtcacgagg atctggcgcc ggcccgcgac gagcccgggc acgaggaggc gtccgtcctc
    21481 gaactcgacg ggcgtcatga cgaggcccgc gggcgcgctc ccgaggcgtt cgggc
//
LOCUS       DAOMFY010000037        21455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_773_length_21455_cov_6.958224, whole genome shotgun sequence.
ACCESSION   DAOMFY010000037 DAOMFY010000000
VERSION     DAOMFY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_773_length_21455_cov_6.958224"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..4496
                     /locus_tag="PLB01_05115"
     CDS             81..4496
                     /locus_tag="PLB01_05115"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQQ76712.1"
                     /translation="MNDPTLDVDHHAARTSSIAARGSRVFVGFEDATYTGAGYGVSGD
                     GGATFSHQRIAMGPGVYAWGSPSAAIGPSGEIYYAFLSSAVGDPTEVTLAKSTDGGLT
                     FPGLSDPSASVQDGFAIMDRPAVAVDGGAASPRKGMVYVAWSYHDAYTPFWAVLVSSS
                     TDGLAFGVPKVFPPAISPGVRVNRPALAVAPNGDLYLAYENGYVSPDGIAVGRSTDGG
                     QSWTLRSLATFQPAGALTAGGGGAASSPGPSIAVDGNGVVHVVWAGISSGSTADRSDV
                     FYARSTDNGATFSAARKLNDDGTATSQAFPAVAALPDGSVAVRWTDRRNDSRDTLNDV
                     YMAISRDGGATFGKNFRVTDRNSPWGPAPHSLAGAGHTSHDGLAADGGTFYVSWTDER
                     SGDADVYAAVVPAGFDAAAPDFDLSARNTYAAVPRGGAATFDLAASGVNGFAGTLALA
                     PDLVPAGLALSLANDTVLPGQAVRLTVTASASAAPGDYVFGVAATSGSLVRGTRMRLT
                     VTDRARGFAPPANVTQKSGFSMGAVKPDGAGVLHAVMDDDSGFVTASEVFYRRSTDGG
                     ATFSAPVKLHPTGARGGQSAFTIDPTGRIFVVWSGRFIADPTPRIYFVKSTDGGATFS
                     TPLALNGNTGYAVLPNVAADRIGNIVVGWYDLSGDVPVVGWSRSTNAGTSFSTPVAVF
                     DSPVVFARPAVAIDAKNNAYLAWTQQTSSTSVIRLAISKAGAAFGAPASVTGPETLSF
                     APDLAAGPDESVGLAFYTRAIVNGTAQNRLATFMRSTTGGTSFSSPVFLSSAAEQSYV
                     PAVAFEPSGAIDVAWEGFDANDVQSDVKVARSTDGGATFGPAVDLSGNGGFSGSSANP
                     VDGLGGPGRAAISAGANGTIVVSWSDDTGGAADVFVTTLPAALISNRPPAATITNPAP
                     NAAFEAGVPVAFSGSATDPDGDTVTLTWDFGDGKSGTGATPAAHPYAVPGTYRVTLTA
                     RDGKGGSSVASITITIGTPTESTGTTRLLPVVLESPGLGGSRYTSEVTLWAGGDSTPV
                     LLSYTAATGGGSGFARLNLGSHEMRTLPDVIAWLRTQNLPIPDDGATHVGTLRITFST
                     HPGEYDYAGARTFTADPAGGTGTFGLAYPSAESTTATATLFGLQQNAAQRSNVAVVNA
                     GPDPIVLRVSLQGPSGEDLGALPDQPLGPWGWFQFNQPLAGKAPSGRAVVTRVSGASP
                     FTAYAVLNDAVTSDGSYVPALLAGDASGADRLVPVVLDVQGLGARFRTELTLTNFTAA
                     PLALQLVYAAANGFGSGSGVVPLTLAAGEQRILPDTIAFLRGTLPIESDGRNVGGSLS
                     VRAAPGTPAGALGVGARTYVPIPSGGSYGLFYPGLTAAQCADTTASVYGLQENASQRS
                     NLAVVNRGDAGDAITLRVTYFGAGPGRPLLGSPVVRTLAPGEWFQFNRPLAELGASAG
                     YAQVEKISGASRFAAYAVLNDNVTSDGSYVAMSFP"
     gene            4528..4713
                     /locus_tag="PLB01_05120"
     CDS             4528..4713
                     /locus_tag="PLB01_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76713.1"
                     /translation="MRKTRLGAALAAALLAAPAVLAGTAAAPRQVLPFIEDDYTKALA
                     SAKASGKPIFFEAWAPW"
     gene            4729..5859
                     /locus_tag="PLB01_05125"
     CDS             4729..5859
                     /locus_tag="PLB01_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76714.1"
                     /translation="MKAFVFTDKALERQAGRFVWLSVNTEKRENAALLGKFPVKVWPS
                     IYVIDPATEKIVLRWAGGVTTPQLLRLLDDGEKAARGKAAPGAALADADRLYADGKYP
                     EAAAAYRKALETQKPGAPSYARTVESLLFSLSQSGQREACAATARDVFPSLRATPSAA
                     NVAGSGLDCALGMKDDDPKKAALVAALAADAREVVEGPKTGLAADDVSAVYGSLADER
                     SAAKDEPGAKKTLEAWVGYLEGEAAKAKTPLERTVFDAHRVSACLALGQPERVVPALV
                     QSEKDFPDDYNPPQRLAIAYGAMKKYDEGLAASDRAMARAYGPRKLVVYRTRAGLYKD
                     KGDAAAAKKTLEEMLGYAEGLPKEQVSERTIASIKKQLEAAN"
     gene            5878..6303
                     /locus_tag="PLB01_05130"
     CDS             5878..6303
                     /locus_tag="PLB01_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQQ76715.1"
                     /translation="MKREFRLDFEIAAPPGRVWAVIRDVERWHEWTASITSVELLDGR
                     PLAVGSRARVRQPNLPVAVWQVTELEEGRSFRWETRGPGVVAAGIHAVAPRGAGSLAT
                     LALEHSGLLGPLFARLTRSITERYVRLEAEGLRRRSESA"
     gene            complement(6505..6852)
                     /locus_tag="PLB01_05135"
     CDS             complement(6505..6852)
                     /locus_tag="PLB01_05135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQQ76716.1"
                     /translation="MNRGEVWWVDLPEPVASAPGGTRPVLVVQSNAFNRSAIRTVVVA
                     AITTNLRLADAPGNVALGAKEAGLPKPSVVNVSQILTLDRTFLRSRSGRLKPATLEAV
                     DRGLHLVLSLVGR"
     gene            complement(6849..7088)
                     /locus_tag="PLB01_05140"
     CDS             complement(6849..7088)
                     /locus_tag="PLB01_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76717.1"
                     /translation="MKTAISIPDAIFREGEKMAARRRVSRSELYVTALKEYLHRHRDD
                     SVTAALNEVYGRAPSALDPGLAALQALSLPSDSWK"
     gene            complement(7131..8363)
                     /gene="mqnE"
                     /locus_tag="PLB01_05145"
     CDS             complement(7131..8363)
                     /gene="mqnE"
                     /locus_tag="PLB01_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HQQ76718.1"
                     /translation="MILDRFKGSALLPIAEKVAAGTRLSFADGVALFESPDVLGVGAL
                     ANFVREKKHGNTGYYNVNRYLNPTNLCWVDCALCAWARKPGVDGGYTMSIEEAVREAA
                     AGWHDGITELHIVGGLHPSLPYSWYLDLLRALRAKFPGVHLKAWTMVEVDWIARVAKK
                     DVVTVIRALKDAGMDSCPGGGAEIFEKRVRDVICRDKISGERWLELAKLCHENGVKTN
                     VTMLYGSVETVEDRVDHLVRIRTLQDETHGFLGFIPLAFHPEETQLPDLPAATGMTDL
                     KVIAVSRLMLDNVDHVKAYWVQIGEKLAPVALHFGADDLVGTVMEETITHAAGATTEA
                     GLTRAQMEALIRGAGREPFERDAFYERVVRDAGGQVLRANRHDFAKREEKDSLKVGEK
                     NPDSFPPAGGLDAAIASS"
     gene            complement(8360..8605)
                     /locus_tag="PLB01_05150"
     CDS             complement(8360..8605)
                     /locus_tag="PLB01_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76719.1"
                     /translation="MSGPVWKRLHQKGACDSCGETFDERYAVGTVFDFTFRPDSAYCA
                     TCYARYGGRADELNPDAVGQMQAPRTLSRPNSVSDVE"
     gene            complement(8616..9908)
                     /locus_tag="PLB01_05155"
     CDS             complement(8616..9908)
                     /locus_tag="PLB01_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ76720.1"
                     /translation="MVETSAMPGDSPPRSRTGLPKTVVRLGWVSFLTDVSSEMIVPLL
                     PAFLVTLSGVPAAALGWIEGVADTTASFVKILSGKWTDRAKAKKPLVFLGYGLSSVAR
                     PLIGLAPGWPTVVLIRFFDRIGKGVRTAPRDTMIAAAAPPDRRGAAFGLHRAFDNAGA
                     VFGPLLAAALVGWMGLSLRSVFLLAAIPAALSLLVLAFGVREEKKGEEGKEKISLKVN
                     TAAAGDAPHDARRGFPQPSLPLEESFSSLPPVFWRTAGVFALFALAASSDTFLLLKAK
                     EIGIAAAWLPLLWAFSNAVKSVFSTWGGGLSDRFGRKRLLLAAWTLYAACYAGFAFVS
                     SPGPLVALVGIYSLYYSLSEGTEKALVADLVGPEQRGRAFGWMNGLIGFAALPASVVF
                     GVIWQGAGSKAAFLAGAGVAAAAVMGLVALVPHGRKAG"
     gene            9889..10524
                     /gene="recO"
                     /locus_tag="PLB01_05160"
     CDS             9889..10524
                     /gene="recO"
                     /locus_tag="PLB01_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQQ76721.1"
                     /translation="MALVSTTAFILKLDPLSEKDLVVALLTKDHGVVRAAVRGARGKS
                     KRAAALQLLTEVSLTYFRKEGADLARVDGLEIVKSAYDLAVSADAAMLLPYVAESALT
                     FVPESELGGDVYRLVRHVLDALEAGVPAPLAARYFETWLLRFAGVLDEEEVSEPARSV
                     LASMRKLPLLALAKKPPPAGALEALEDLLREARRGFLGHELKSYRFLQTLG"
     gene            10554..11516
                     /gene="glyQ"
                     /locus_tag="PLB01_05165"
     CDS             10554..11516
                     /gene="glyQ"
                     /locus_tag="PLB01_05165"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HQQ76722.1"
                     /translation="MDLQTLILRLSAYWSSKGCLIEQPWDVEVGAGTMHPATFLRVLG
                     PEPWNAGYVQPSRRPADGRYGQNPFRLVKHQQFQLVMKPAPVDIQDLYVESLVALGID
                     PKEHDLRFEEDNWESPTLGAWGVGWQVLLDGMEITQFTYFQQAGGIDLAPITAEITYG
                     LERLTMFLAKKRSVYDIEWAPGVTYGEARKQDEYEVSKYGFEVADPALWQLLFEKYEA
                     EAKRCLEAGLVLPAYDFTLKCSHAFNILDARGAVSATDRVGVIKRVRGLAVECAKAYV
                     ASREAQDFPLLPKKPEPEAPPDDAPAADAPASDPIAPAPEPSDV"
     gene            11509..13569
                     /gene="glyS"
                     /locus_tag="PLB01_05170"
     CDS             11509..13569
                     /gene="glyS"
                     /locus_tag="PLB01_05170"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQQ76723.1"
                     /translation="MSEFLLEILAEEIPAGVLPGAREDLLARVSNAFAEARLGGTLAV
                     HSTSRRLILTGEDVAERQPDATLEVTGPPAVAAFDADGKPKKAAEGFAKAQGVSVDDL
                     SVVQLPKGAYVVARKTVPGRPASEVISEILPPLVEKMTFPRMMRWGDGTPTWIRPVHS
                     VVALLDGLVIPLHLFGVESGRTTYGHRTLAGGRLIVMGVGDWFAKLRAARVEPDLAVR
                     RARFEEQARALAAEIGGEPAADASLFEAWSHLVEWPGLVRGAFDEAFLELPEEILVTS
                     MREHQKMLPIRREGALLPAFLAVCDQTGDPKGLIAQGNEWVTSARFADARFFWDDDGK
                     TKLEDRLSRLGRLQFQETLGDYLMKTGRVQDLAERFAARLSVADSSSVVRAARLMKAD
                     LVTDMVREFPDLQGVVGGLYAKREGERDEVWQAIYDQYRPASADDASPRSDVGAVVAL
                     ADRLDTLTGLFGLGLVPTGSRDPYALRRAALGVVKILLDKGWHLDIPVAISDALQLHV
                     GLARGRQEVVPELTAFLTERLRFLLEKRGHAADTIDAVLSADARDPVDAAERVAAVDA
                     MRKEADFAPLAAAFKRMQNILAQAPDALGEPDPAKMTDAAEQALAGDYLQARGMIDDL
                     LSQRHFHDALGIMASFGPALDRFFTEVLVMAEDEGVKANRIALLKAIRDQFARVAKFA
                     EIRG"
     gene            13575..13973
                     /locus_tag="PLB01_05175"
     CDS             13575..13973
                     /locus_tag="PLB01_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76724.1"
                     /translation="MRRIGLLIVAALAVLSLLGFPSGCDSSPTEPDGNGRFSPTPTFT
                     PTSPSFTPPPTPTLGNPVTATPTPIVTGTPPTPTPTSGLGSTPISPTPTFTPVPPTPT
                     SPSPTATPRTPVPTATPFAGTPTPVQTPTR"
     gene            complement(13981..14781)
                     /locus_tag="PLB01_05180"
     CDS             complement(13981..14781)
                     /locus_tag="PLB01_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76725.1"
                     /translation="MPKPRPHSLSKKSSAKRGRKGPPMRKLQPHSPSKKSSNSPKSPK
                     SSPKAKKPSSAPVFPTLEVVFESVEVKHNGWPGGAGTPTDLSLILQLEIPSAAAPSTA
                     LASARMPFRAASGQTVAADDLPPDASGRPFFGGPIRVSNHVNLHARLFVDRTNTVGPI
                     LGAALNTVIGTFVGQIPLLGSPLREVLHIQIGNVISTELARATAIVPADETMNGTHPV
                     TIALVAPRSIPGVYAPPGSPEGYRKGVVVTEGDLVALLHLGLTLTLPR"
     gene            14924..15490
                     /locus_tag="PLB01_05185"
     CDS             14924..15490
                     /locus_tag="PLB01_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HQQ76726.1"
                     /translation="MKRATLFVLAAAALAAVIPARPAAAKELAGVTMPETLSVGDKTL
                     KLNGLGLRKKAIFKVYVGGLYLEAPSKDAAAILASDGAKAVRMTFLRDVSKSQLKDGF
                     VEGFEANAKEKAAAQKAAIEKLYGLIPDMKETQTLSFLYLPGKGTIVSHGDKELGVIE
                     GKEFSEALFALWLGPKPPSEDLKKGMLG"
     gene            15487..17574
                     /locus_tag="PLB01_05190"
     CDS             15487..17574
                     /locus_tag="PLB01_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HQQ76727.1"
                     /translation="MTGGASLQSLLTEKRFDLVETVLQEALADPLLNEETILAALRGL
                     ARAGQKPRLQALAGAADAALKKSRDPDAPRLRWTILKEAVRAGATPSTPDGFHRLFED
                     VLAAAWPDAASLASLLGRFKFREAKDPLDGLARAEKAEAWLPFETGKVFAMAGRGAGK
                     VVETNFALNVVRLDFEAAKGVAVPIGIASRQLVPLPPGHFLNEKFTAPDALRERVVAD
                     PGAALKHLIDSFGRALTMTEVKEAVKGLVAEDAWTSWWTASKKNPQVVVHGSGKNATV
                     EWSDSADAADATLLAKFERSALKDRIDLFRKNQRRSADLALSMAKLLVRDAEELEDRD
                     SATAFEIAVLVEKVPGVDHATDVERHVLAQPLALLGRLSDRSIRERALEILVREKPAE
                     APGVLAEWMFKEEDGRTIELIDRKLAEIDPATRERTLDKLLKNPRSGPRAFVWFAQRS
                     ATDDAFRARLNPNVLGRLLDAISWDELGGLRTKVREMFDRTGLVAGWLVKQASVEEAR
                     TFLAGLSRHHELEPLRRDGLLAAAEMRFKDLRKAEDDTFFVTAEAIERKRAELDDILK
                     VQIPENTKGIALAAAEGDLSENFEYKARRDKQQLLSARAGKIQEELGKARALDPATID
                     TTEIRPGSRVTLEGPKGKKTVTLLGPWDSKPDEGVYSYLSELGKLLLGKAVGEQATVL
                     GDEMTIKKIEVFV"
     gene            17591..19621
                     /locus_tag="PLB01_05195"
     CDS             17591..19621
                     /locus_tag="PLB01_05195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ76728.1"
                     /translation="MKISLKVEEAMRVNHGTGGGWAAAPLHLLRKSSLPLLLLSSIFF
                     SPLPARGADDAVVPNEALVLDGLPAVPKALAERATAYMEFRTASFQAWNPLRPEMLVT
                     TRFGDVPQLHRVKAPGAARTQLTFYSERILGARYRPKTADIVFSKDVGGGEWFQLYLR
                     EDRTGKVTLLTDGRSRNQGGAFSRDGTWMAYTSTKRTGKNGDIWVMNPSDPAKTDRMV
                     VEGSGENLFVTDWSPDAKTLLVVEGISVNESRQWLVDVASGAKTRFSPESKELVSWGG
                     GRFSPDGKAVYTTTDLDSEFKRLVAVDVATKKMDVLTPGLAWDVQGFALSEDGTKLAW
                     TVNEAGAETLHAMDVRTRKEIPLPKVPYGTIGGLGWSPEARNLGFTLTSAKSPADAWA
                     VDTQTGVLSRWTESETGGLDANAFAEPEIVSWKSFDGKTITGLLYRPPASFAGPRPVI
                     VNIHGGPEGQTQAGFLGRNNFFLNELGVAILYPNVRGSTGYGKTFVKLDNGVLREDSV
                     KDVGALLDWIAAQPSLDKSRVMVTGGSYGGYMTLAAMTHYNDRFRCALDVVGISNWVT
                     FLEHTESYRRDLRRVEYGDERDPKMRAFLERISPLASASKITKPMFIVQGKNDPRVPY
                     TEAEQMVGAIKKNGGPVWYLLAKDEGHGFSKKKNQDFLFLATLKFVEDNLLK"
     gene            19731..>21455
                     /locus_tag="PLB01_05200"
     CDS             19731..>21455
                     /locus_tag="PLB01_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ76729.1"
                     /translation="MRTTHGTGSGRAAARLHLLGKSSLLFLPIFLFFFVLTAFPSSAA
                     ELPHVYALVNARVVTSAGKVIDKGTVIVRGGVIAAVGAAGAVAVPADAHVMDLEGQTV
                     SPGLIDPYVTVSRLSGVRPPASDDSSAGRRGAGAAAFPQATPAPEPSGNALPSSKLRP
                     ERTVLSDLKVSSDAREAFRDLGFTVVAAVPDKGILRGESAVLALSDGPLSRALLVAKS
                     AQHVARDADAPEFGGEYPGSKMGMAAAVRQAFSDAKWLPAADAAYAAKPAGRARPERY
                     DAWVALGDAAAGKEPVVFEAPDVLALLRYGKIAAEFGLKARYVATGSDLYVLADEVRA
                     AKPDLVLSLAFPAAPSVEDEDEWSDVSLQRLRAWDRAASNPRWMRDLGLTFSLTTHGL
                     PELSDLADRAKRARARGLSADDLLAAFTTVPARQLGLAGRAGEIVPGALANLVVSSGP
                     LFMERTRVLTTFVDGVPYEVRPKKGAVSGTFKVDDASVALRTDPRTGQPGVGVTPAGG
                     GKPVAATNVVRHGQRLTFDVDGAPLGLSGPQKAEAVVEGDSLRLDVRSAAGGPLVSKR
                     GERDRAASG"
BASE COUNT         3200 a         7605 c         7553 g         3097 t
ORIGIN      
        1 tccgggaggc cgccgccggc gctccgcgga ccgggtccgc cgcgggcctc gccgcgcccg
       61 ctccgcgcca gaacgtccgc gtgaacgacc cgaccctcga cgtcgaccac cacgcggcgc
      121 gtacttcctc catcgcggcc cgcggctctc gcgtcttcgt gggattcgag gacgcgacgt
      181 acacgggggc gggctacggc gtgtccggcg acggcggcgc gacgttctcc caccagcgga
      241 tcgccatggg ccccggcgtc tacgcgtggg ggagcccgtc ggccgccatc ggcccgtccg
      301 gcgagatcta ttacgcgttc ctctcgtccg cggtcggcga cccgaccgag gtcacgctcg
      361 cgaagtcgac ggacggcggc ctgacgttcc ccgggctctc ggacccttcc gcctccgtcc
      421 aggacggctt cgccatcatg gaccggcccg cggtcgccgt ggacggcggc gccgcgtccc
      481 cgcggaaagg gatggtgtac gtggcctggt cgtaccacga cgcctatacg ccgttctggg
      541 cggtcctcgt gtcgtcctcg acggacggcc tcgcgttcgg cgtgccgaag gtctttccgc
      601 ccgccatcag cccgggagtc cgggtcaacc gtccggccct cgccgtcgcc ccgaacggag
      661 acctctacct cgcgtacgag aacggctacg tgtcgcccga cgggattgcg gtcgggcgct
      721 cgacggacgg gggccaatcg tggacgctcc gctcgctcgc gacgttccag cccgcaggcg
      781 cgctgaccgc gggcggcggg ggcgccgcgt cgagccccgg gccctccatc gccgtcgacg
      841 gaaacggcgt cgtccacgtg gtctgggccg ggatctcttc aggctcgacc gcggaccggt
      901 cggacgtgtt ctacgcccgg tcgaccgaca acggggcgac gttctcggcc gcgcgaaagc
      961 tgaacgacga cgggacggcg acgtcgcagg cttttccggc cgtcgccgcc ctgccggacg
     1021 ggagcgtcgc cgtgcgctgg acggaccggc gcaacgactc ccgggacacg ctgaacgacg
     1081 tgtacatggc gatctcgcgc gacggcggcg caaccttcgg aaagaacttc cgggtcacgg
     1141 accggaattc cccgtggggg cccgccccgc acagcctggc gggtgccggc cacacgtccc
     1201 acgacgggct cgcggcggac ggcgggacct tctacgtctc gtggacggac gaacgctcgg
     1261 gtgacgccga cgtgtacgcg gcggtcgtgc ccgctgggtt cgacgcggcg gctcccgact
     1321 tcgacctctc ggcccggaac acgtatgcgg ccgtcccgcg gggcggcgcc gcgaccttcg
     1381 acctcgcggc ctccggcgtc aacggcttcg ccgggacgct cgcgctcgcg ccggacctcg
     1441 tgcccgcggg gcttgcgctg tctctcgcga acgacacggt tctcccgggc caggccgtcc
     1501 gcctgacggt gacggcgtcg gcctccgcgg cgccgggcga ctacgtcttc ggcgtcgccg
     1561 cgacgtccgg aagcctcgtc cgcgggacgc gcatgcgcct gacggtcacg gaccgcgcgc
     1621 gcggcttcgc gccgcccgcg aacgtgacgc agaagtccgg gttctcgatg ggggcggtca
     1681 agccggacgg ggcgggcgtg ctccacgccg tcatggacga cgactccggg ttcgtgacgg
     1741 cctcggaggt cttctaccgg cgctcgaccg acggcggcgc gacgttctcc gcgcccgtga
     1801 agctccaccc gacgggcgcc cgcgggggtc agagcgcgtt cacgatcgac cccacgggcc
     1861 ggatcttcgt cgtctggtcc ggccggttca tcgccgaccc gacgccgcgg atctacttcg
     1921 tgaagtccac cgacggcggg gccacgttct cgacgcctct cgccctgaac gggaacacgg
     1981 gctacgcggt cctcccgaac gtcgccgccg accgcatcgg caacatcgtc gtcggctggt
     2041 acgacctctc cggggacgtg ccggtcgtgg gctggtcgcg ctcgacgaac gcggggacca
     2101 gcttctcgac gcccgtcgcg gtcttcgact cgccggtcgt cttcgcgcgg cctgccgtcg
     2161 cgatcgatgc gaagaacaac gcgtacctcg cctggaccca gcagacctcg agcacgtccg
     2221 tgatccggct cgcgatctcg aaggcgggcg cggcgttcgg cgctcccgcc tccgtgacgg
     2281 gcccggagac cctttcgttc gcgccggacc tcgccgccgg ccccgacgaa agcgtcggcc
     2341 tcgcgttcta cacgcgcgcg atcgtgaacg gcaccgccca gaaccggctc gccacgttca
     2401 tgcgttccac gaccggcggg acgtcgttct cgagcccggt gttcctgtcg tcggcagccg
     2461 aacagtccta cgtgcccgcc gtcgccttcg aaccctcggg tgcgatcgac gtcgcgtggg
     2521 aggggttcga cgcgaacgac gtccagagcg acgtcaaggt cgcgcgctcc acggacggcg
     2581 gggcgacgtt cggccctgcg gtcgacctct cgggaaacgg gggcttctcg ggcagctccg
     2641 cgaaccccgt cgacggcctc ggcggccccg gccgggcggc gatctcggcc ggcgcgaacg
     2701 ggacgatcgt cgtctcgtgg tccgacgaca ccggcggcgc ggcggacgtg ttcgtcacga
     2761 cgctccccgc ggcgctgatc tcgaaccgcc cgcccgccgc gacgatcacg aaccctgccc
     2821 cgaacgcggc gttcgaggcc ggcgtgcccg tggcgttctc gggctcggcg acggatcccg
     2881 acggcgacac cgtgacgctg acctgggact tcggggacgg caagagcggg acgggcgcga
     2941 cgcccgcggc gcacccctac gcggtgccgg gcacgtaccg cgtcacgctg accgcgcgcg
     3001 acgggaaggg cggttcttcc gtcgcgtcga tcacgatcac gatcggcacc ccgaccgagt
     3061 ccacggggac cacgcgcctt ctcccggtcg tcctcgagtc gccggggctg ggcgggagcc
     3121 gctacacgag cgaggtcacg ctctgggcgg gcggcgattc gacaccggtc ctcctttcct
     3181 acacggccgc cacgggcggg ggcagcggat tcgcgcgact caacctcggc tcccacgaga
     3241 tgcggacgct gccggacgtc atcgcgtggc tccggaccca gaacctcccg atcccggacg
     3301 acggcgcgac gcacgtcggc acgcttcgca tcacgttctc cacgcatccg ggcgagtacg
     3361 actacgcggg agcccgcacg ttcacggccg atcccgcggg gggaacgggg acgttcggcc
     3421 tcgcctatcc gtcggcggag tcgacgacgg cgaccgcgac gctcttcggg ctgcagcaga
     3481 acgcggccca gcgctcgaac gtcgccgtcg tgaacgccgg gccggatccg atcgtcctcc
     3541 gggtctccct ccagggcccg tcgggcgagg acctcggcgc cctgccggac cagccgctcg
     3601 gcccgtgggg ctggttccag ttcaatcagc ccctcgcggg aaaggcgccg tcgggccggg
     3661 ccgtcgtgac gcgtgtctcc ggcgcgtccc cgttcaccgc gtacgcggtc ctgaacgacg
     3721 ccgtgacgtc ggacgggtca tacgtgccgg cgctcctcgc gggagacgcg tccggcgccg
     3781 accgcctcgt gccggtcgtc ctcgacgtgc aagggctcgg cgcgcgcttc cggacggagc
     3841 tgacgctcac gaacttcacg gcggcgccgc tcgcgctcca gctcgtctac gccgccgcga
     3901 acggtttcgg cagcgggtcg ggcgtcgttc cgctcacgct cgccgcgggg gagcagcgca
     3961 tcctgccgga cacgatcgcg ttccttcgcg ggacgctccc gatcgagagc gacggccgga
     4021 acgtcggcgg ttccctctcc gtccgggcgg caccgggcac gccggcgggc gctctcggtg
     4081 tcggcgcgag gacgtacgtt ccgattccgt ccggcggctc gtacggcctg ttctatccgg
     4141 gcctcacggc ggcccagtgc gcggacacga ccgcctccgt ctacggcctt caggagaacg
     4201 cctcccagcg gtcgaacctc gcggtcgtca atcgcggcga cgcgggcgac gcgatcacgc
     4261 tccgcgtgac gtatttcggg gcgggcccgg gcaggccgct gctcgggtcg ccggtcgtca
     4321 ggacgctcgc gccgggcgag tggttccagt tcaaccggcc gctcgccgag ctcggggcct
     4381 ccgcgggcta cgcgcaggtc gagaagatct cgggcgcgtc gcggttcgcc gcgtacgctg
     4441 tcctgaacga caacgtgacg tccgacggca gctacgtggc gatgagtttc ccgtaacctt
     4501 gcgcgaccgg gtccgtatga tcggaccatg agaaagacac gactcggggc ggcgctcgcc
     4561 gccgcccttc tcgccgctcc ggccgtgctg gccggaacgg cggccgcgcc ccgccaggtc
     4621 ctgcccttca tcgaggacga ctacacgaag gcgctcgcct ccgcgaaggc ctccggcaag
     4681 ccgatcttct tcgaggcctg ggcgccctgg tgacacacgt gtcgctcgat gaaggccttc
     4741 gtcttcacgg acaaggcgct cgagcggcag gccggacggt tcgtctggct ttcggtcaac
     4801 accgagaagc gtgaaaacgc cgcgctgctg gggaagttcc ccgtcaaggt ctggccttcg
     4861 atctacgtga tcgacccggc gaccgagaag atcgtgctgc gctgggcggg cggcgtcacg
     4921 acgccgcagc tcctccgcct cctcgacgac ggggagaaag cggcgcgcgg aaaggcggcg
     4981 cccggcgcgg cgctcgcgga cgcggaccgt ctctacgcgg acgggaagta ccccgaggcc
     5041 gcggcggcgt accgcaaggc gctcgagacg cagaaaccgg gcgcgccgtc gtacgcgcgc
     5101 acggtcgaat cgctcctctt ctccctctcg cagagcgggc agcgggaggc ctgcgcggcc
     5161 acggcgcgcg acgtcttccc gagcctccgg gcgacgccgt cggccgcgaa cgtcgcgggc
     5221 tcgggtctcg actgcgcgct cggcatgaag gacgacgatc cgaagaaagc ggcgctcgtg
     5281 gcggccctcg ccgcggacgc gcgcgaggtc gtcgagggcc cgaagacggg cctcgcggcc
     5341 gacgacgtct cggccgtcta cggctcgctc gccgacgagc gcagcgccgc gaaggacgag
     5401 ccgggcgcga agaagacgct cgaggcgtgg gtggggtacc tcgagggcga ggccgcgaag
     5461 gcgaagacgc cgctggagcg caccgtcttc gacgcgcacc gcgtgtccgc gtgtctcgcg
     5521 ctgggccagc ccgagcgcgt cgtcccggcg ctcgtccagt cggagaagga cttcccggac
     5581 gactacaacc cgccgcagcg cctcgcgatc gcgtacgggg cgatgaagaa gtacgacgag
     5641 ggcctcgcgg cgtccgaccg cgcgatggcg cgggcctacg gtccgcgcaa gctcgtcgtc
     5701 taccgcacgc gcgccggcct ctacaaggac aagggcgacg cggcggcggc gaagaagacg
     5761 ctcgaggaaa tgctcgggta cgcggaggga ctgccgaagg agcaggtcag cgagcgcacg
     5821 atcgcgtcga tcaagaagca gctggaggcg gcgaactagg gagggcgcgc gcgcaccatg
     5881 aaacgcgagt tccgcctcga cttcgagatc gccgctccgc ccgggcgcgt ctgggccgtc
     5941 atccgcgacg tggagcgctg gcacgagtgg accgcctcga tcacgagcgt cgagctcctc
     6001 gacggccggc cgctcgccgt cggcagccgc gcccgcgtcc gccagccgaa cctccccgtc
     6061 gccgtgtggc aggtcacgga gctggaagag ggacggtcgt tccgctggga gacgcgcggc
     6121 cccggcgtcg tcgccgcggg gatccacgcg gtcgcgccgc gcggggccgg gagcctcgcg
     6181 acgctcgctc tcgagcactc cggtctcctc ggcccgctct tcgcgcgcct cacgcgatcg
     6241 atcacggagc ggtatgtccg gctggaggcg gagggactca ggcgccgaag cgaatccgcc
     6301 tgatagtctg acgccactcc tcgattccga ccgcaggagg cgtcctgggc gttctcgatt
     6361 tcctccgaag acggggcccg ttgtgaagga cggggagacg gtcggatcga gcgaggacga
     6421 gcggatcacc gttcgccttc ttccgggccg tcgggagccg gacaaaacgg tccacaagct
     6481 cgtctttggt tcctgacgcg cgcgtcagcg tcctacgagc gagaggacga ggtgaagccc
     6541 gcggtccacg gcttcgagcg ttgcgggctt gaggcggccc gagcgcgaac gcaggaacgt
     6601 tcgatcgagc gtcaggatct gggagacgtt tacgacggac ggctttggaa ggccggcctc
     6661 cttcgcgccg agcgcgacgt ttccgggagc atccgcgagg cggaggttcg tggtgatcgc
     6721 ggcgacgacg accgtgcgaa tcgcactcct gttgaaggca ttcgactgga cgacgagaac
     6781 gggccgggtt ccgcccggcg ccgacgcgac gggttcgggc agatcgaccc accagacctc
     6841 gccgcggttc acttccatga atcggagggg agcgagaggg cctgaagggc ggcgaggccg
     6901 ggatccagag ccgagggcgc acgcccgtac acctcgttga gggcggcggt cacggagtcg
     6961 tcccgatggc gatgcagata ctccttgagc gccgtgacgt acagctcgct gcgcgagacg
     7021 cgccgccgcg cggccatctt ctctccctcg cggaagatcg cgtccgggat ggaaatggcg
     7081 gtcttcatga gctgagtata actctggtta taccgaagtc aaggcgccac tcagctcgac
     7141 gcgatggcgg cgtcgaggcc gccggccggc ggaaaggaat ccgggttctt ctctccgacc
     7201 ttcagagaat ccttctcttc tctctttgcg aaatcgtgcc tgttggcccg cagcacctgg
     7261 ccgccggcgt cccgcacgac gcgctcgtag aacgcatcgc gctcgaaggg ctcgcggccg
     7321 gcgccgcgga tgagcgcctc catctgcgcg cgcgtgaggc cggcttccgt cgtcgcgccg
     7381 gcggcgtgcg tgatggtctc ctccatcacg gtgccgacga ggtcgtccgc gccgaagtgg
     7441 agcgcgaccg gggcgagctt ctcgccgatc tgcacccagt acgccttcac gtggtcgacg
     7501 ttgtcgagca tgagacgcga gacggcgatg accttcaggt ccgtcatgcc ggtggcggcg
     7561 gggaggtcgg gcagctgagt ctcctcgggg tggaacgcga gcgggatgaa cccgaggaag
     7621 ccgtgcgtct cgtcctgcag cgtgcggatc ctcacgagat ggtcgacgcg gtcctcgacg
     7681 gtctcgacgc tgccgtagag catcgtgacg ttcgtcttga cgccgttctc gtggcagagc
     7741 ttcgcgagct cgagccagcg ctcgcccgag atcttgtcgc ggcagatgac gtcgcggacg
     7801 cgcttttcga agatctccgc gccgccgccc gggcaggagt ccatgccggc gtccttcagc
     7861 gcgcggatga ccgtgacgac gtccttcttc gcgacgcggg cgatccagtc cacctcgacc
     7921 atcgtccacg ccttcaggtg gacgcccggg aacttcgcgc gcagcgcgcg caggaggtcg
     7981 aggtaccagg aataggggag cgagggatgg agtcccccga cgatgtggag ctccgtgatc
     8041 ccgtcgtgcc agccggccgc ggcctcgcgg acggcctcct cgatcgacat cgtgtagccg
     8101 ccgtcgacgc cgggcttgcg ggcccacgcg cagagcgcgc agtccaccca gcagaggttc
     8161 gtcgggttca ggtaccggtt gacgttgtag tagccggtgt tgccgtgctt cttctcgcgg
     8221 acgaagttcg cgagcgcgcc gacgccgagg acgtcgggcg actcgaagag cgcgacgccg
     8281 tcggcgaacg agaggcgcgt ccccgcggcc accttctccg cgatcgggag gagggccgac
     8341 cccttgaacc ggtcgaggat cactcgacgt cgctgacgga gttcgggcgc gacagggtgc
     8401 gcggcgcctg catctggccg accgcgtccg ggttcagctc gtccgcgcgg cccccgtagc
     8461 gggcgtagca cgtcgcgcag taggccgagt cgggccggaa cgtgaagtcg aagacggtcc
     8521 cgacggcgta acgctcgtcg aacgtctctc cgcaggagtc gcatgcgccc ttctggtgga
     8581 ggcgcttcca cacgggtcca gacatggcgg ggatcttacc cggccttgcg cccgtgcgga
     8641 acaagcgcca cgaggcccat gacggcggcc gcggcgacgc cggcccctgc caggaacgcg
     8701 gccttcgaac cggcgccctg ccagatcacg ccgaagacga cgctcgccgg gagggccgcg
     8761 aacccgatga ggccgttcat ccagccgaag gcccggccgc gttgctcggg cccgacgagg
     8821 tcggccacga gcgccttctc cgtcccttcc gagagcgagt agtagagcga gtagatcccg
     8881 acgagcgcga cgagcgggcc cggcgaggac acaaacgcga agccggcgta gcaggccgcg
     8941 tagagcgtcc acgcggcgag gagcaggcgc ttgcggccga agcggtcgga cagcccgcct
     9001 ccccacgtcg agaacacgga cttcacggcg ttcgagaacg cccagaggag ggggagccac
     9061 gcggcggcga tcccgatctc cttcgccttg aggaggagga acgtgtcgga cgacgccgcg
     9121 agggcgaaca gcgcgaacac ccccgcggtc cgccagaaga ccgggggaag agaagagaaa
     9181 gattcttcga gtggtaaaga aggctgcggg aagccgcggc gcgcgtcgtg cggcgcatcg
     9241 cccgccgctg ccgtattcac cttcaaagaa atcttctctt ttccctcttc tcctttcttc
     9301 tcttctctca ctccgaaagc aagaaccaga agcgagagcg cggcgggtat cgcggccaga
     9361 agaaagacgc tgcgcaggga gagtcccatc catccgacca gggcggccgc gaggaggggg
     9421 ccaaagacgg cgcccgcgtt gtcgaaggcg cggtgcaggc cgaaggcggc gccgcggcgg
     9481 tccggcggcg cggcggccgc gatcatcgtg tcgcgcggcg cggtgcgcac gcccttgccg
     9541 atgcggtcga agaagcggat gaggacgacc gtcggccagc cgggcgcgag gccgatcagg
     9601 gggcgggcga cggacgagag tccgtagccg aggaaaacga gcggcttctt cgccttcgcg
     9661 cggtccgtcc acttgcccga gaggatcttc acgaacgagg ccgtcgtgtc cgcgacgccc
     9721 tcgatccagc ccagcgccgc cgcgggcacg cccgacagcg tcacgaggaa cgcgggcagg
     9781 agcgggacga tcatctcgga cgacacgtcc gtcaggaaag acacccaccc gaggcggacg
     9841 acggtcttcg gcagcccggt ccggctccgc gggggagaat cgccgggcat ggcgctcgtc
     9901 tcgaccacgg ccttcattct gaagctcgat ccgctgtcgg agaaggatct cgtcgtggct
     9961 ctcctcacga aggaccatgg cgtcgtccgg gcggccgtgc gcggcgcgcg cggcaagtcg
    10021 aagcgcgcgg cggcgctcca gctcctcacc gaggtctcgc tcacgtactt ccgaaaggag
    10081 ggcgcggacc tcgcgcgcgt ggacgggctc gagatcgtga agagcgcgta cgacctcgcg
    10141 gtgagcgccg acgcggcgat gctgctgccg tacgtcgcgg agagcgcgct caccttcgtg
    10201 cccgagtccg aactcggcgg agacgtctac cggctcgtgc gccacgtcct cgacgctttg
    10261 gaggccggcg tcccggcgcc gctcgcggcg cggtacttcg agacgtggct gctgaggttc
    10321 gcgggcgtgc tcgacgagga ggaggtcagc gagcccgcga ggagcgtcct cgcgtccatg
    10381 cgcaagctcc cgctcctcgc gctcgcgaag aagccgcccc ccgcgggcgc cctcgaggcg
    10441 ctcgaggacc tgctgcgcga ggcgcgccgg ggatttctcg ggcacgagct gaagagctat
    10501 cggttcctcc agaccctcgg ctgagcgggg attcggtaca ctcagggccc ctcatggacc
    10561 ttcagacgct gatcctcagg ctctcggcct actggtcgtc caagggctgc ctcatcgagc
    10621 agccgtggga cgtcgaggtc ggcgccggga cgatgcaccc ggccacgttc ctgcgcgtcc
    10681 tcggccccga gccgtggaac gccggctacg tccagccctc gcgccgcccg gcggacggcc
    10741 gctacggcca gaaccccttc cggctcgtga agcaccagca gttccagctc gtcatgaagc
    10801 cggcgccggt cgacatccag gacctctacg tcgagagcct cgtcgccctc gggatcgacc
    10861 cgaaggagca cgacctccgc ttcgaggagg acaactggga gtccccgacg ctcggcgcgt
    10921 ggggcgtcgg ctggcaggtg ctcctcgacg ggatggagat cacgcagttc acgtacttcc
    10981 agcaggcggg cgggatcgac ctcgcgccga tcacggccga gatcacgtac ggcctcgagc
    11041 gcctcacgat gttcctcgcg aagaagcgga gcgtctacga catcgagtgg gcgcccggcg
    11101 tcacctacgg cgaagcccgc aagcaggacg agtacgaggt ctcgaagtac ggcttcgagg
    11161 tcgcggaccc cgcgctctgg cagctcctct tcgagaagta cgaggcggag gcgaaacgct
    11221 gcctcgaggc cgggctcgtc ctcccggcgt acgacttcac gctgaagtgc tcgcacgcgt
    11281 tcaacatcct cgacgcgcgc ggcgccgtct cggcgacgga ccgcgtcggc gtcatcaagc
    11341 gcgtgcgcgg cctcgccgtc gagtgcgcga aggcgtacgt cgcgtcgcgc gaggcgcagg
    11401 acttcccgct gctcccgaag aagccggaac cggaggcgcc gcccgacgac gcgccggccg
    11461 ccgacgcgcc ggcctccgat ccgatcgcac cggcgccgga gccgtccgat gtctgaattc
    11521 ctgctcgaga tcctcgccga ggagatcccc gcaggcgtcc ttccgggcgc gcgcgaggac
    11581 ctgctcgccc gcgtctcgaa cgcgttcgcc gaggcccgcc tcgggggcac gctggccgtc
    11641 cactccacgt cgcgccgcct gatcctcacc ggagaggacg tggccgagcg ccagcccgac
    11701 gccacgctcg aggtcaccgg gccgccggcg gtcgcggcct tcgacgcgga cggaaagccg
    11761 aagaaggccg ccgagggctt cgcgaaggcg cagggcgtgt ccgtcgacga tctttcggtc
    11821 gtccagctcc cgaagggagc ctacgtcgtc gcgaggaaga ccgtccccgg ccgcccggcg
    11881 tcggaggtca tctccgagat cctgccgccg ctcgtcgaga agatgacgtt tccgcgcatg
    11941 atgcgctggg gcgacggcac gccgacctgg atccggcccg tccactccgt cgtcgcgctc
    12001 ctcgacggcc tcgtcattcc gctccatctc ttcggcgtcg agagcggccg cacgacgtac
    12061 ggccaccgca cgctcgcggg cggcaggctc atcgtgatgg gcgtcgggga ctggttcgcg
    12121 aagctgcgcg ccgcccgcgt cgagcccgac ctcgccgtgc gccgcgcgcg tttcgaggag
    12181 caggccaggg cgctcgcggc cgagatcggc ggcgagcccg ccgcagacgc gtcgctgttc
    12241 gaggcgtggt cgcacctcgt cgagtggccg ggcctcgtgc gcggagcgtt cgacgaggcg
    12301 ttcctggagc ttccggaaga gatcctcgtg acgtcgatgc gcgagcacca gaagatgctc
    12361 ccgatccgcc gcgagggggc gctgctgccc gcattcctcg cggtctgcga ccagacgggc
    12421 gacccgaagg ggctcatcgc gcagggcaac gagtgggtca cgtcggcgcg gtttgccgac
    12481 gcgcgcttct tctgggacga cgacggcaag acgaagctcg aggaccgcct ctcgcgcctc
    12541 gggcgcctcc agttccagga gacgctcggc gattacctca tgaagacggg ccgcgtgcag
    12601 gacctcgccg agcgcttcgc ggcgcgcctc tcggtcgccg attcgtcgtc ggtcgtccgc
    12661 gccgcccgcc tcatgaaggc cgacctcgtc acggacatgg tccgcgagtt ccccgacctg
    12721 cagggcgtcg tgggagggct ctacgcgaag cgcgagggcg aacgcgacga ggtgtggcag
    12781 gcgatctacg accagtaccg gcccgcgtcc gccgacgacg cgtcgccgcg gagcgacgtg
    12841 ggcgccgtcg tcgcgctcgc ggaccgcctc gacacgctca cgggcctctt cggcctcggc
    12901 ctcgtcccga cgggctcgcg cgacccgtac gcgctgcgcc gcgcggcgct cggcgtcgtg
    12961 aagatcctcc tcgacaaggg ctggcacctc gacatcccgg tcgccatctc ggacgcgctc
    13021 cagctccacg tcgggctcgc gcgcgggcgg caggaggtcg tgccggagct gacggcgttc
    13081 ctgacggagc ggctgcgctt cctcctcgag aagagggggc atgcggccga cacgatcgac
    13141 gcggtgctct cagcggacgc gcgcgacccg gtcgacgccg ccgagcgcgt cgccgccgtg
    13201 gacgcgatga ggaaggaagc cgacttcgcg ccgctcgccg cggccttcaa gcggatgcag
    13261 aacatcctcg cgcaggcgcc cgacgcgctg ggcgagccgg accccgcgaa gatgacggac
    13321 gcggccgagc aggcgctcgc gggcgactac ctccaggccc gcgggatgat cgacgacctc
    13381 ctctcgcagc gccacttcca cgacgcgctc ggcatcatgg cgtcgttcgg gcccgccctc
    13441 gaccgcttct tcaccgaggt cctcgtcatg gccgaggacg agggcgtgaa ggccaaccgc
    13501 atcgcgctcc tgaaggcgat ccgcgaccag ttcgcgcgcg tcgcgaagtt cgccgagatc
    13561 cggggctgag gcacgtgcgg cggatcgggc tcctgatcgt cgccgcactc gccgtcctct
    13621 ctcttctggg gtttccgtcg ggctgcgact cgtccccgac ggagcccgac ggaaacgggc
    13681 ggttctcgcc gacgccgacc ttcaccccga cgtccccgtc gttcactccg ccccccacgc
    13741 cgacgctggg gaacccggtc acggcgacgc cgacgccgat cgtcacgggc acgccgccga
    13801 ctccgacgcc gacgagcggc ctcgggtcga ctccgatctc gccgacgccg actttcaccc
    13861 cggtgccgcc gacgcccacg tcgccgtccc cgaccgcgac gccgaggacg cccgtgccga
    13921 cggcgacgcc gttcgcgggc actccgacgc cggtgcagac gccgacgcgc tgatcgcgcc
    13981 tcagcgcggg agcgtgagcg taagcccgag gtggaggagg gccacgaggt cgccttccgt
    14041 gacgacgact cccttgcggt agccctccgg gctccccggc ggcgcgtaga cgcccggaat
    14101 cgagcgcgga gccacgagcg cgatcgtgac ggggtgcgtt ccgttcatcg tctcgtcggc
    14161 cgggacgatc gccgtcgcgc gcgccagctc ggtggagatc acgtttccga tctggatgtg
    14221 gaggacttcg cgcagggggg agcccaggag agggatctgg ccgacgaacg tcccgatgac
    14281 ggtgttcagg gcggcgccga ggatcgggcc gaccgtgttc gtgcggtcca cgaagaggcg
    14341 ggcatgaagg ttcacgtggt tcgagacgcg gatggggccc ccgaagaacg ggcgcccgga
    14401 ggcgtccggg ggcaggtcgt ccgcggcgac ggtctgcccg gacgccgccc ggaacggcat
    14461 gcgtgccgag gcgagcgccg tgctcggcgc ggcggccgag gggatctcga gctggaggat
    14521 gagcgagagg tccgtcggcg ttccggcgcc gccgggccag ccgttgtgct tgacctcgac
    14581 ggactcgaag acgacctcga gggtggggaa gacgggggcc gaagagggtt tctttgcttt
    14641 cggagaagat ttcggagatt tcggagaatt cgaagatttc ttggaaggtg aatggggctg
    14701 cagcttgcgc atcggcggcc ccttacggcc acgtttcgcg gaagatttct tagaaagtga
    14761 gtggggccgc ggcttgggca tcgtccacct cgtctcgctc cggcggtgca gtccgattct
    14821 ttcaccttct aagaaaatca gcgtttccgt cgctgcggcg cctcacggag cgcccgggtc
    14881 cgaagcaaag ctagaatccc cgttccattt gaggaggatt gcgatgaaac gcgccaccct
    14941 gttcgtgctc gccgctgcgg ctctcgccgc cgtcatcccc gcccgcccgg ccgccgcgaa
    15001 ggaactcgcg ggcgtgacca tgcccgagac cctctccgtc ggcgacaaga cgctgaagct
    15061 gaacggcctc ggcctcagga agaaggccat cttcaaggtc tacgtcggcg gtctctacct
    15121 cgaggcgccc tcgaaggacg ccgcggcgat cctcgcctcc gacggggcga aggcggtgcg
    15181 gatgacgttc ctgcgcgacg tctcgaagag ccagctgaag gacggcttcg tcgagggctt
    15241 cgaggccaat gcgaaggaga aggccgccgc ccagaaagcc gccatcgaga agctctacgg
    15301 cctgatcccg gacatgaagg agacccagac gctctccttc ttgtacctcc ccggcaaggg
    15361 cacgatcgtc tcccacggcg acaaggagct cggcgtcatc gagggcaagg agttctccga
    15421 ggccctcttc gcgctgtggc tcggaccgaa gcctccgtcc gaggacctca agaagggaat
    15481 gctcggttga cgggcggcgc ctcgctgcag agccttctca ccgagaagcg cttcgatctc
    15541 gtcgagaccg ttttacagga ggcgctcgcc gaccctctcc tgaacgagga aacgatcctc
    15601 gcagccctgc gcggcctcgc gcgcgccggc cagaagcctc gcctgcaggc cctcgcgggc
    15661 gcggcggacg cggcccttaa gaagtccagg gaccccgacg ccccgaggct ccgctggacg
    15721 atcctcaagg aggccgtccg ggccggagcg acgccgtcga cgcccgacgg cttccaccgc
    15781 ctcttcgagg acgtgctcgc ggcggcctgg cccgacgccg cgtcgctggc gagcctcctc
    15841 ggccggttca agttccgcga ggcgaaggac ccgctcgacg gcctcgcccg cgccgagaag
    15901 gccgaggcct ggctgccgtt cgaaaccggc aaggtcttcg cgatggccgg gcgcggcgcc
    15961 ggcaaggtcg tggagaccaa cttcgcgctc aacgtcgtgc gtctcgactt cgaggccgcc
    16021 aagggcgtcg cggttccgat cggcatcgcg tcccggcagc tcgtgccgct tccgccgggc
    16081 cacttcctga acgagaagtt cacggcgccg gacgccctgc gcgagcgcgt cgtggccgac
    16141 cccggggcgg ccctcaagca cctcatcgac tccttcggac gcgccctgac catgaccgag
    16201 gtcaaggaag ccgtgaaggg cctcgttgcc gaagacgcct ggacgtcctg gtggaccgcc
    16261 tcgaagaaga acccgcaggt cgtcgtccac gggagcggca agaacgcgac ggtcgagtgg
    16321 tccgactcgg ccgacgccgc cgatgcgacg ctcctcgcga agttcgagcg gtccgccctg
    16381 aaggaccgca tcgacctctt ccgcaagaac cagaggcgct cggcggacct cgcgctctcg
    16441 atggcgaagc tcctcgtgag agacgccgag gagctcgagg accgggactc ggccacggcg
    16501 ttcgagatcg ccgtcctcgt cgagaaggtg cccggcgtgg accacgcgac ggacgtggag
    16561 cgccacgtcc tcgcccagcc cctcgccctt ctcgggcgcc tctcggaccg gtcgatccgc
    16621 gagcgggcgc tcgagatcct cgtccgcgag aagccggcgg aagcgcccgg cgtcctcgcg
    16681 gagtggatgt tcaaggagga ggacggccgg acgatcgagc tcatcgaccg caagctcgcc
    16741 gagatcgacc cggcgacgcg cgagcggacc ctcgacaagc ttctcaagaa cccgcgttcg
    16801 ggcccgcgcg ccttcgtgtg gttcgcgcag cggtccgcca cggacgacgc gttccgggcc
    16861 cgcctgaacc cgaacgtcct cggccggctc ctcgacgcga tctcgtggga cgagctcggc
    16921 ggcctgcgca cgaaggtgcg cgagatgttc gaccgcacgg gcctcgtggc gggctggctc
    16981 gtcaagcagg cctcggtgga ggaagcgcgc accttcctcg ccgggctctc gcgccaccac
    17041 gagctcgagc cgctgcgccg ggacggcctc ctcgcagccg ccgagatgcg cttcaaggac
    17101 ctccgcaagg cggaggacga cacgttcttc gtgacggcgg aggccatcga gcgaaagcgc
    17161 gccgagctcg acgacatcct gaaggtgcag atccccgaga acacgaaggg gatcgcgctc
    17221 gcggccgcgg agggcgacct ctcggagaac ttcgagtaca aggcccggcg cgacaagcag
    17281 cagctcctct cggcccgcgc cggcaagatc caggaagagc tcggaaaggc ccgggcgctc
    17341 gaccctgcga cgatcgacac gaccgagatc cgccccggct cgcgcgtgac gctcgagggc
    17401 ccgaagggga agaagaccgt cacccttctc ggtccgtggg actcgaagcc cgacgagggc
    17461 gtgtactcgt acctctccga gctcggcaag ctcctgctcg ggaaggccgt gggcgagcag
    17521 gcgacggtgc tcggggacga gatgacgatc aagaagatag aggtctttgt ttaggaagat
    17581 tcggattcgg atgaagattt ctttgaaggt ggaggaagca atgcgcgtca atcacggcac
    17641 cggcggcggg tgggctgcgg cccctcttca ccttctaaga aaatcttctc ttcctcttct
    17701 tctcttaagc agcattttct tctcccctct tccggcgcgg ggcgcggacg acgccgtcgt
    17761 gccgaacgag gcgctcgtcc tcgacggcct gccggccgtg ccgaaggcgc tcgccgagcg
    17821 cgcgaccgcg tacatggagt tccggacggc gtcgttccag gcctggaacc cgctgcgtcc
    17881 cgagatgctc gtcacgacgc gcttcggcga cgtcccgcag ctgcaccgcg tcaaggcgcc
    17941 cggcgccgcg cgcacgcagc tcacgttcta ttcggagcgc atcctcggcg cgcgctaccg
    18001 gccgaaaacg gccgacatcg tcttctcgaa ggacgtcggc ggcggcgagt ggttccagct
    18061 ctacctgcgc gaggacagga ccgggaaggt cacgcttctc acggacggca ggtcgcgcaa
    18121 ccagggaggc gccttctcca gggacgggac gtggatggcc tatacgtcca cgaagaggac
    18181 ggggaagaac ggcgacatct gggtcatgaa tccttccgac cccgcgaaga ccgaccggat
    18241 ggtcgtcgag ggctcgggcg agaacctgtt cgtgaccgac tggtcgccgg acgccaagac
    18301 gctcctcgtc gtcgagggaa tctccgtcaa cgaatcgcgg cagtggctcg tggacgtcgc
    18361 gtcgggcgcg aagacgcgct tctcgccgga gtcgaaggaa ctcgtgtcgt ggggcggggg
    18421 ccgcttctcg cccgacggga aagccgtcta cacgacgacg gacctcgatt cggagttcaa
    18481 gcgcctcgtc gccgtggacg tcgcgacgaa gaagatggac gtcctgacgc cgggtctcgc
    18541 ctgggacgtg cagggcttcg ccctctccga ggacgggacg aagctcgcgt ggacggtcaa
    18601 cgaggccggc gccgagacgc tgcacgcgat ggacgtgagg acccgcaagg agatcccgct
    18661 cccgaaggtc ccgtacggga cgatcggcgg cctgggctgg tctcccgagg cccggaatct
    18721 cggcttcacg ctcacgtccg cgaagtcgcc tgccgacgcc tgggccgtcg acacacagac
    18781 gggcgtgctg agccgatgga ccgagagcga gacgggcggc ctcgacgcga acgcgttcgc
    18841 ggagcccgag atcgtgtcgt ggaagagctt cgacggaaag acgatcacgg gcctcctcta
    18901 ccgtccgccg gcgtccttcg ccggcccgcg ccccgtcatc gtgaacatcc acggggggcc
    18961 ggagggacag acccaggcgg gcttcctcgg gcggaacaac ttcttcctga acgagctcgg
    19021 cgtcgcgatc ctctacccga acgtgcgcgg ctcgacgggc tacggcaaga cgttcgtcaa
    19081 gctcgacaac ggcgtcctcc gcgaggactc cgtgaaggac gtcggcgcgc tcctcgactg
    19141 gatcgccgcg cagccttctc tcgacaagag ccgggtcatg gtgacgggcg gctcgtacgg
    19201 cggctacatg acgctcgcgg ccatgacgca ctacaacgac cgcttccgct gcgcgctcga
    19261 cgtcgtcggg atctcgaact gggtgacgtt cctcgagcac accgagagct accgccgcga
    19321 cctgcgccgc gtcgagtacg gcgacgagcg cgacccgaag atgcgcgcgt tcctcgagag
    19381 gatctcgccc ctcgcgagcg cgtcgaagat cacgaaaccc atgttcatcg tgcagggcaa
    19441 gaacgacccg cgcgtgccgt acaccgaggc cgagcagatg gtcggcgcga tcaagaagaa
    19501 cggcggcccc gtgtggtacc tgctcgcgaa ggacgaggga cacggcttca gcaagaagaa
    19561 gaaccaggac ttcctcttcc tcgcgacgct gaagttcgtc gaggacaacc tgctcaagta
    19621 ggaagaagaa gagaagaaga gaagtcttct tgcttcggac ccgagcagcg cctccaggcc
    19681 ggagcgccac gggccgatgt tctcggattt cttggaaggt ggaggttgcg atgcgcacca
    19741 ctcacggcac cggcagcggg cgggcagcgg cccgtcttca ccttctagga aaatcttctc
    19801 ttctctttct cccgatcttt ctcttcttct tcgttcttac tgcatttccg tcctcggcag
    19861 cggagctgcc gcacgtgtac gcgctcgtga acgcgcgcgt cgtgacttct gccggcaagg
    19921 tcatcgataa gggcacggtg atcgtgcgcg gcggcgtgat cgcggccgtc ggcgccgcgg
    19981 gcgcggtcgc cgtgccggcc gacgcgcacg tgatggacct cgagggccag acggtgtcgc
    20041 cgggcctcat cgacccgtac gtcacggtct cgcgcctctc cggcgtccgc cctcctgcct
    20101 ccgacgactc gtccgcgggc cggcgcggcg cgggcgcggc cgcgttcccc caggccacgc
    20161 cggcgcccga gccctccggc aacgcgctcc cgtcgtcgaa gctccgcccg gaacggacgg
    20221 tcctctccga cctgaaggtt tcctccgacg cacgcgaggc gttccgcgac ctcggcttca
    20281 ccgtggtcgc cgccgtgccc gacaagggca tcctgcgcgg ggagagcgcc gtgctcgccc
    20341 tctccgacgg cccgctctcc cgcgcgctcc tcgtcgcgaa gtcggcccag cacgtcgcgc
    20401 gcgacgccga tgcgcccgaa ttcggagggg agtaccccgg atcgaagatg gggatggcgg
    20461 ccgcggtgcg gcaggccttc tccgacgcga agtggctccc cgcggccgac gccgcgtacg
    20521 cggcgaagcc cgccgggcgc gcgcgcccgg aacgctacga cgcgtgggtc gcgctcggcg
    20581 acgcggccgc cggcaaggag cccgtggtct tcgaggctcc ggacgtcctc gcgctcctcc
    20641 gttacgggaa gatcgcggcg gagttcggcc tgaaggcgcg ttacgtggcg acggggagcg
    20701 acctctacgt gctcgccgac gaggtccgcg cggcgaagcc cgacctcgtc ctctcgctcg
    20761 cgttcccggc cgcgccgtcg gtcgaggacg aggacgagtg gagcgacgtg tcgctccagc
    20821 gcctgcgcgc gtgggaccgc gcggcctcga acccgcgctg gatgcgcgac ctcggcctga
    20881 cgttctcgct gacgacgcac ggcctcccgg agctctccga cctcgcggac cgcgcgaaga
    20941 gggcccgggc ccgcggcctt tcggcggacg acctcctcgc ggccttcacg acggtcccgg
    21001 cgcgccagct cggtctcgcc ggccgcgcgg gcgagatcgt ccccggcgcg ctcgcgaacc
    21061 tcgtcgtgtc gtccggcccg ctcttcatgg aaaggacacg cgtcctcacg acgttcgtcg
    21121 acggcgtccc gtacgaggtg aggccgaaga agggcgccgt gagcgggacg ttcaaggtgg
    21181 acgacgcttc ggtggccctg cgcaccgacc cgcgcaccgg gcagccgggc gtcggcgtga
    21241 cgcccgcggg cggaggcaag ccggtcgcgg cgacgaacgt cgtgcggcac ggccagcggc
    21301 tcacgttcga cgtcgacggc gcgcctctcg ggctgtccgg cccgcagaag gcggaggccg
    21361 tcgtggaggg cgactcgctg cggctcgacg ttcgttccgc cgcgggcggg cctctcgtct
    21421 cgaagagggg cgagcgggac cgcgccgcgt cgggc
//
LOCUS       DAOMFY010000038        20682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_820_length_20682_cov_9.654143, whole genome shotgun sequence.
ACCESSION   DAOMFY010000038 DAOMFY010000000
VERSION     DAOMFY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20682
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_20682_cov_9.654143"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLB01_05205"
     CDS             <1..459
                     /locus_tag="PLB01_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76730.1"
                     /translation="AHPKYYSYVGLVAPQDKKAVYMGYAFLYGVIGSLIGSSLGGAMY
                     AAWLKPLVGQSGTEAATRGFWLVFVVLDVAAMIGLVAYDRVFAADTPATNEKARKVMV
                     GVYALLLLVGAAFLVRSLSGPEMSPRTLVQAVIMLALGGGGMAVSLGRKR"
     gene            complement(538..723)
                     /locus_tag="PLB01_05210"
     CDS             complement(538..723)
                     /locus_tag="PLB01_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76731.1"
                     /translation="MTGTAVGIRGGGATARAVGAAEGAGVGAPIRSTIDGGGGPSSGT
                     AAGVAATDWARIRIASR"
     gene            713..1075
                     /locus_tag="PLB01_05215"
     CDS             713..1075
                     /locus_tag="PLB01_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQQ76732.1"
                     /translation="MPVTAPALAAPVPKAAPSAPPVPSARPVPAAPQKAAASRASAFG
                     VHVSSFRKREIAEADGRRWTARLGLPARVLEVDLGEKGVWYRVVVGEAGSVAEASVLR
                     DELKGKGAPDGLVQGFPR"
     gene            1072..1524
                     /locus_tag="PLB01_05220"
     CDS             1072..1524
                     /locus_tag="PLB01_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76733.1"
                     /translation="MRTRRRLLVPLVALAVVVPVLRLRAEGPAPTPRATPDSRLADAR
                     ALVRSGRAADGRKILAGVVAADPKNRDARKALLEAAALMKDWNECNAQTALLEPFADG
                     EEPSMFYAAVALRETGSIASARALVQRARPFLVPSPFVDWYTKEILGN"
     gene            complement(1542..1817)
                     /locus_tag="PLB01_05225"
     CDS             complement(1542..1817)
                     /locus_tag="PLB01_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76734.1"
                     /translation="MDTTSAPNPSRAYDADIAAATAHLKESWEYGKEAARDARRIAKK
                     KFEDLSGGIDDYVESRPVTIALWALGAGIVAGALLGLLVRRALHRSA"
     gene            complement(2119..4107)
                     /locus_tag="PLB01_05230"
     CDS             complement(2119..4107)
                     /locus_tag="PLB01_05230"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQQ76735.1"
                     /translation="MTHRVLLALALAFSPGPEALPAAPVSAERVEKDTPRTTPAGATF
                     TAPAGWTAAVKGPLVVLRPPEGDSSLALVDSEAKDADAAVAAAWAAYAGGPKWPLKLS
                     TPQASRNGWEERRIYQYETSPNEKKTVFAGAFRAGKAWTVVIVDASDATFEKRGGPFS
                     LALNSLRPKGYAKESFAGKKAHPVDAKMVAALKAFLADGMKQFDVPGVGFSLIDGGKV
                     VFEGGLGVRDLGKPAPVDADTLFIAASNTKALSTLLVAEQVDAKKMRWDQPVTEVYPA
                     FRLGDAATTKQVLVKHLVCACTGMPRQDLEWLFEYRNASPASAMTLLGTMQPTSKFGE
                     VFQYSNLMAAAAGFIAASLEMPGKELGTAYDEAMRKRVFLPLGMTNTTFDFAKALSGI
                     VAQPHGDDVDGNTVRARIDLNESVVPVRPAGGVWTSPRDLSRYVQMELAKGLLPDGTR
                     LVSEENLLARRAPQVLVSEDVTYGMGLFVDTRWGITIVHHGGDLAGYHSDMFWLPDYG
                     IGGVILANSDAGVSLRGPFLRKLVELLFDGKPEADAQIKAGAAARKASIKKERERLVV
                     PADAAAVARLAPRYVSTALGDVSVTRKGTATIFDVGEWKSTVASRKNDDGTVSFITID
                     PTISGFEFVVADKDGRRRLVMRDAQHEYAFEEAGPKAP"
     gene            complement(4172..5524)
                     /locus_tag="PLB01_05235"
     CDS             complement(4172..5524)
                     /locus_tag="PLB01_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ76736.1"
                     /translation="MSHRTCVALVLSFVVAARASAQQPAPAPVPQPAVTAVTAVKAGR
                     LVDPETGKISANQVILVEGEKIKAVGAGAAVPAGATVIDLSDLTVLPGLVDTHTHLGL
                     TYKDVPEHDYYYLTYVMDSTPLRAIQAASNGIQLLNSGFTVVRDLGNNALYTDTAHRV
                     AIDQGWIPGPTMVNSGLIIGGKGGQFWPTPEMAKHHGIVYPEYLEADTPDEIVKAVRE
                     NMLFGAKVIKICVDCKPWGYTVDEMKLFVSEAAKGGLKVAGHVQTPEGATRAIEAGIF
                     SIEHGMALTEAHHKAMAQKGIFLASTDTPFTPYHGTKDGMEKNAAQLRSAWKNGVKVT
                     FSTDMDYYKTGNPMNRGELTINFLLTWKAAGIPAPDILKAMTTTGYACAQVEKERGAI
                     RPGLAADFIAVRGNPLEDIDTLRDVRFVMKDGLVFKQGGVMTPEKFFHAGPAKGWRKR
                     "
     gene            complement(5562..6206)
                     /locus_tag="PLB01_05240"
     CDS             complement(5562..6206)
                     /locus_tag="PLB01_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQQ76737.1"
                     /translation="MTPVLVSVAFAAAATITVAAHALGRQPVVYVFKPLSTALLLLLV
                     AAGSSVLSSRYAAAVFGGLFFSLLGDVFLMLPSDRFRSGLFCFLLAHASYVFAFLQDS
                     PLATPLLPFAVWIAVGSAVFRVLWPGIPRRLRLPVAAYVAILLVMAGQASSRAGNLHT
                     APALQTAFGAALFVLSDSLLAWNRFRKPFPGAQALVHASYFTAQWLIAIAACRT"
     gene            complement(6203..6859)
                     /locus_tag="PLB01_05245"
     CDS             complement(6203..6859)
                     /locus_tag="PLB01_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HQQ76738.1"
                     /translation="MNLFRARRLFGIVLLLQPFAAGVAIAADLPPALELPRPKLDSPA
                     TLVSALKARRSAREFSPAPLSVQALSDLLWAADGVNRDDGRRTAPSAKNWQEIDVYAV
                     LPGGAYVYDAKAHALRGVAKGDLRAATGVQDFVTAAPLNLVYVADLSRMSGASGGDRD
                     LYAAADAAFVSQNVYLWCAATGLSTVVRGSVDRPALAKVLGLRPDQRILLAQTVGMPK
                     "
     gene            complement(7234..7584)
                     /locus_tag="PLB01_05250"
     CDS             complement(7234..7584)
                     /locus_tag="PLB01_05250"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HQQ76739.1"
                     /translation="MSVYVEFSKPIDDYTEVSSLRFGMAAGTLAGTGRQASANDVNFS
                     MDVNSMSRKIWQACMNGTTFSAVWVEFYKNEDSDEPYMVYTLSDVLVLSVSTHGTTMH
                     VGLNFTKAKGKYAR"
     gene            7874..9337
                     /locus_tag="PLB01_05255"
     CDS             7874..9337
                     /locus_tag="PLB01_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76740.1"
                     /translation="MKRVLTTSVLFAFALTSAHAARAVDASEQGPSTWSAPAFFAPPA
                     AAHSAGGRQSLAAGPGVPLPFVAVTPCRLVDTRGNGAPLQGGFLPAATVRTYTLVGVC
                     NIPADAKAISLNATAVNPVGPGFLTLYAAGASLPTVSTLNFVANTVVANAAVVPLSAS
                     GAISVVFGVSGGDLVLDTNGYYSPLGVVNSLNGQGGDLTLVAGSNITLTPGAGTLSIS
                     AASGTGPQGPAGPTGATGPTGPQGPTGATGTFDTSCVVSATDYAALRDCLLAPATVFD
                     SIPSPTPPNVPSEGFQCCQNSEVGDQITLAGTARKAVSATVLMSSWSLLSTYPGMSAA
                     GYTHPITLNLYSDASHAAAHTPDLGSVTQNVLVPWRPPADPTCPGGTAWRAGNGSCYN
                     GYAFPVVFSLSGITLPSTFVYGIAYNTNTWGYVPIGAGGPYESLNVGLNSTPPIAVGT
                     DVDVDAVWRSMGKPSGPFAPETGWTPYTPAVKFKATN"
     gene            9433..10359
                     /locus_tag="PLB01_05260"
     CDS             9433..10359
                     /locus_tag="PLB01_05260"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HQQ76741.1"
                     /translation="MARLVLHEVGPRDGLQVEKQIVPTATKRAWIERLLDSGVDIVQV
                     GSFVNPEKVPQMADTDELFRQLAARPAGKGVLSGLVLNEKGLDRALLCGVPLVCLGVS
                     ASDTHSRKNTGMDTTEATRRIVATARKAAEAGRAVQVSVQSAFGCGFEGPVPEERVLS
                     IVKEYLAAGLRRISLADTAGHAYPEQVERLYAAILALAPDVEPACHFHDTYGLGMANV
                     WAAMRAGAVSFESSFAGLGGCPFTKVAAGNVATEDLVAGWQRSGIRTDVDLAVLIEVA
                     RSVAAHFGREMPGRVHKTGPVPTAPKKVEVPA"
     gene            10356..11555
                     /locus_tag="PLB01_05265"
     CDS             10356..11555
                     /locus_tag="PLB01_05265"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQQ76742.1"
                     /translation="MTRKALEGIRVLDLTNVLSGPYATLHLALLGAEVVKIENPADGD
                     LARKLGNVPKLNKELMGTSFLAQNANKKSITLNLKKKEAKEIFLRLVAESDAVVENFR
                     PGVMTRLGVGWDAMRAVNPRLVYCAISGFGQDGPDADKPAYDQIIQGLSGTMSVNGDE
                     RLNPLRCGFPVCDTVGGLTAAFAIMTALYHRERTGEGQMIDVALLDAIMPLMGWVAAN
                     LLIGGQQPVLMGNDNFTAAPSGVFRTGDGFINIAANKHEQWEAVCDALGVPELKTDAR
                     FAERDTRKKNRKDLTPLLEAKLAGKPTKEWVALLNAKDVPSGEILGLDEALHQPQVAH
                     RGTLTTVDAPGIGPLPLFNLPALFGATPAAVEAPPPRLGAHTEEILGRLGLSASDLTN
                     LKQSGAI"
     gene            11600..12979
                     /locus_tag="PLB01_05270"
     CDS             11600..12979
                     /locus_tag="PLB01_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76743.1"
                     /translation="MPHTLVIVGSLIVFVLVLSWLVPSGAYQTMEKAGRQVTVPGTYQ
                     RVAKVVLGPQWLVIAPIRGFLDGSLIIAFLILIGGAFNVIQTTGIVEQGIRKITAALA
                     SRPRLEKLMVPALMTIFSLAGSVFGMSEEVIPFVLICVPLALSLGYDSIVGVSIPFLG
                     AAAGFGAAFFNPFTVGIAQGLVGLPLYSGLGYRVITWFVCTGVIVGWVMVYAARVKRR
                     PESSPVYALDRARDRSAFDASAPPEPWTVRRILVVLLFLAGMVALVWGILKAQWFIEE
                     IGALFLAMGLLMGAVAGLRPGEIATSFVAGAKDMVNVTLIIACGRALLIIAREARVLD
                     TILYASSGAISMLPTVVAAQVMFVVQAVINFFIHSGTAQAALTMPIMAPLADLVGLTR
                     QTTVYAYQLCELINPILPTSAVTMGVLGMAKIPWETWARWFLPLMLTLCALSFLLLVP
                     PVLMHWGPF"
     gene            12980..14473
                     /locus_tag="PLB01_05275"
     CDS             12980..14473
                     /locus_tag="PLB01_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HQQ76744.1"
                     /translation="MASFAPLLLAAALAAGEPAALPEDHRKLEKTISYAQMETFLRSV
                     DGKGPVRVSVEGRTAQGRAVYLVHLSTAAPGVPPAWKVLFYAQQHGDEISGKDALLYL
                     VRDIAAKPSLLPPDVDLWILPMMNPDGAEAGTRRNASGADLNRDHLALDQPETQALHR
                     VVRRVRPHVAVDSHEFGRDSKAWRAKGWRKWPDITMDGLNNPLFDRGVVAAARRWVDE
                     GAAAEADAGHPFLRYWVGEPPPDGEQRHSSPDVDSGLNAVGMYGGLSFIIEAAAYESG
                     APAGDLGNRVDAYLVLYRCFLAGGSRRAEDLATIERARRRPLPAFLPTNYLWVNPDAK
                     VTEFPVVEASTGRVLKVPTANLMTDMAVKTTVPTPLGYAISPGAAEVFKTLLDRHGIP
                     FETLAAPRTVKAETATLLRVEDTFDEVYSRYAGRQIVRRGEAHAAELAAGSLWIPLEG
                     EAGVRAALVLEPAALYGLYEHPKFRALAGKDGTLPVLRVVRPQAANP"
     gene            14470..15618
                     /gene="menC"
                     /locus_tag="PLB01_05280"
     CDS             14470..15618
                     /gene="menC"
                     /locus_tag="PLB01_05280"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HQQ76745.1"
                     /translation="MTRIPAALDALLAEKVPAVDRADLVLVRLPFVSPFAISTAVWTT
                     KDALLLRLESGAVAGWGECVADPDPFYAYETTGTARHILKDFLLPRLEPGITLGELER
                     RMRHVRGHEMARATLENAVLDLVAKAKGVPLHELLGAPRKRIPSGISIGLQESPAALV
                     AAVADAVAKKYHRVKMKVQKGKDLDYVRAVRERFPALPLMVDANGDYRLDDAAHLARL
                     DAFGLTMIEQPLSYDDVWQHALLQKSLETPLCLDESIHSLDDAAAAIALGGCRILNLK
                     QGRVGGILESMRILEHAASHGIPVWSGGMDETGIGRAVNIHLQTLDGFTLPGDTSETS
                     RYFHEDLVEPPVVLDAEGFIDVPEGSGFGARVVDERLARLTLSEERIF"
     gene            complement(15655..16551)
                     /locus_tag="PLB01_05285"
     CDS             complement(15655..16551)
                     /locus_tag="PLB01_05285"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HQQ76746.1"
                     /translation="MKARLPLAVLLASLFASPACTQAPAPAAAPPAARTGNLVLIGGG
                     DKPDDAMRKFVELAGGPDAPIVAIPTASSEEDAADYYERLFREKYGCTKTVSLRIKTR
                     EDAARADYAELAGKARGVFFGGGDQVRITKALLDTPVGKAIAEAYARGAVVGGTSAGT
                     ACQSKLMITGEGDFTSIRAKSVEVLEGLGFFPGVVVDQHFVKRQRENRLFSVILEHPE
                     LLGVGVDETAAIWVRPDRTFQVMGKSSVMVFDAAGAAVARQPDAAGKDLLGVHGLKVH
                     VLLPGEVFDLTARKVVKEPAPK"
     gene            complement(16616..19369)
                     /locus_tag="PLB01_05290"
     CDS             complement(16616..19369)
                     /locus_tag="PLB01_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ76747.1"
                     /translation="MTRKISIAVGLALVLLLGSPGAALAQGALTGSLQGVVRDDQGLA
                     VPGVTVVVSSDALVRGKATTTTDSAGVYRFPSLPPGTYRLEATLTGFRTVQRSGLRVG
                     LGQALAVDLKLSLDSVKTEVTVTAEAPIVSVVSNAVSNNFGQDFIEKQPLPRNYYSIV
                     KTAPGVNADVTGNGSAILAYGGTQSRQNAYTIDGVNVADSGGGGYWLLPSIQWMDEIQ
                     VSGLGANAEYGAYTGGIINGVTKSGGNTFHGGLEYYYQPEAWTSNNAPEGFDNGGSFK
                     FQDAALSIGGPIAKDKLWFFASAEYWQQVTTPVGALADTDRKIPRFLGKLTYQPTDKL
                     RMSAMGEYDAVTQERRGVSAYTLPEASSKQDGPNATFALNSEYLVNSSNFLTLKLTGY
                     DGRDDYLPYGGTSLPGRIDDDSGYAWVNQDIHQFDHRHNITLDGSWSLYKDGLFGKTD
                     SHAFKFGGSFESATASDEWLRNGGFTYYDYSGDCAGGLTEYFKNPGCGPYYIERGYGE
                     YNLHGQQTGIVFYAQDSMRLDRVTVNAGIRYTHYDAGFQPGYGNTSVYKVDNWDPRIG
                     IVWDVFGNARTAVKAHWGRYHAGMFTYMYDREVSGNVAIPNQDCYWESSTKGYTDCDP
                     KTSISATMGTVNHPYVDETIFTVEHQTSKDVALGFDFIDRRFRSMMAMVNVNNDYSMV
                     TAKNNPLTGGDLPIYILNSPTKFALTTDNGSYRDYDSLVLRFEKRYSHGWSLRSSVVW
                     TDLKGNIASNSGYANEYRDKNGFTNIDGRIALSYAEWEFKLSGAVDLPLGFVASGQYT
                     YFSGQYWTPYVRVNRGLNYNSSTGRDITLVSRGSEQFDDRNLVDLRLAWGMNFSGVGR
                     LELSLECFNALNKGTVLDNYNRWGTYDARASRQTWTKASNYGAPYTIEQPRQVRAGAR
                     FTL"
     gene            19484..20164
                     /locus_tag="PLB01_05295"
     CDS             19484..20164
                     /locus_tag="PLB01_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76748.1"
                     /translation="MRGGIGPKLYVAWATLAVGGSIALAGLTAESVTRLLVLGFLGAQ
                     LVFRESLAAALPSLAPRARFVVMGTLLAAVVEGFHMISTPVFPSLRVGRGTPPLQALE
                     SFALDLLFTVPAYLVIFSVICFFVSRYRYGTWAYVVVMGFAQAIGDGGLFFFASAPAM
                     LVFLPYPMTNYHAVNVLPFLAVRGQLDPERPSSARSWLVVPAVVGTYLVCGALIKVAG
                     RWSGLEPR"
     gene            complement(20124..>20682)
                     /locus_tag="PLB01_05300"
     CDS             complement(20124..>20682)
                     /locus_tag="PLB01_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HQQ76749.1"
                     /translation="ARAPAAPPTPTSVPPARTLAEGPVDVSLWSEVRVKEWKESLAVD
                     RRTPLAILKMPRLKIDVPVLEGTDDLTLNRGAGWIEGTSRPGETGNVGLSAHRDGFFR
                     ALKDVAAGDEVVLETPRETTRYAVAWTKIVDPDDVSVLDETGGAAVTLVTCYPFYYVG
                     SAPQRLIVRATPLTAAPVPTSGPRP"
BASE COUNT         3030 a         7094 c         7224 g         3334 t
ORIGIN      
        1 gcgcacccga agtactacag ctacgtcggg ctcgtcgcgc cccaggacaa gaaggccgtc
       61 tacatgggct acgcgttcct ctacggcgtg atcggctcgc tcatcggctc gagcctcggg
      121 ggcgcgatgt atgccgcgtg gctgaagccg ctcgtggggc agagcgggac ggaagcggcg
      181 acgcgcgggt tctggctcgt cttcgtcgtg ctcgatgtcg cggcgatgat cggtctcgtc
      241 gcgtacgacc gcgtctttgc ggcggacact cccgcgacga acgagaaggc ccggaaggtc
      301 atggtgggcg tctacgcgct gctgctcctc gtcggtgcgg cgtttctcgt tcggtcgctc
      361 tccggccccg agatgtcgcc gcggacgctc gtccaggccg tcatcatgct ggcgctcggc
      421 ggcggcggca tggccgtcag cctggggcgg aagcgatgac ggattccccc gaggctgagg
      481 gtcccccatt cgaagccgtc gccctcccgc ctcgcgggta agatcgttct cggggcttca
      541 tctgctcgcg atcctgatcc tcgcccagtc cgtcgcggcg acacccgcgg ccgtcccgga
      601 cgacgggccg cccccgccgt cgatcgtcga gcggatcggt gcgccgaccc ccgccccttc
      661 tgcggcgccg acggcgcgcg cggtcgcccc gccgcctctg attccgacgg cggtgccggt
      721 cacggctccc gctctcgcgg ctcccgttcc gaaggctgcc ccttctgcgc cgcccgttcc
      781 ttccgcccgc cccgttccgg ccgcgccgca gaaagccgcc gcgtctcgcg cgagcgcgtt
      841 cggtgtccac gtgagctcgt tccgcaagcg cgagatcgcc gaggccgacg gcaggcgctg
      901 gaccgcgcgg ctcgggcttc cggcgcgcgt cctcgaggtc gacctggggg agaaaggcgt
      961 gtggtatcgc gtcgtcgtcg gcgaggcggg ttcggtggcc gaggcgtcgg tgctccgcga
     1021 cgaactgaag ggaaagggcg ctcccgacgg cctggtccag ggcttccctc gatgaggacc
     1081 cggcgccggc tcttggtccc gctcgtcgcg ctcgccgtcg tcgtccccgt gctccggctg
     1141 cgcgcggaag gtccggcgcc gacgccgcgc gcgactccgg acagccgcct tgcggacgcg
     1201 cgggctctcg tgaggagcgg cagggccgcc gacggccgga agatcctcgc gggcgtcgtt
     1261 gcggcggatc cgaagaaccg cgacgcgcgc aaggcgctgc tcgaggccgc ggccctcatg
     1321 aaggactgga acgaatgcaa cgcgcagacg gctctcctcg agccgttcgc ggacggcgaa
     1381 gagccgagca tgttctacgc cgcggttgcg ctccgggaaa ccgggagcat cgcgagcgcc
     1441 cgcgcgctcg tccagcgcgc gcggccgttt ctcgttccga gccccttcgt cgactggtac
     1501 acgaaggaga tcctcgggaa ctgacctcgg gcggccgccc gtcaggccga tcgatgcagg
     1561 gcgcggcgta cgagaaggcc gaggagggcg cccgccacga tcccggcgcc gagcgcccag
     1621 agcgcgatcg tcacggggcg ggactccacg tagtcgtcga tcccgccgga gaggtcctcg
     1681 aacttctttt tcgcgatccg tcgcgcgtcg cgcgccgcct ccttgccgta ttcccagctc
     1741 tccttcaggt gcgcggtggc ggcggcgatg tcggcgtcgt acgcgcggga cgggttcggg
     1801 gcgctcgtcg tgtccatgtg gtccccccgg ggaacgaaac ccctcttgag agaaggtggg
     1861 cgtccgaggg gagaaggtca aggtggagct acgcgatcgc ggtccattaa gcgaactggg
     1921 cggcgctgga tccgaacgag gcgttcacga aggagcagcg tgagttccag gagaagcact
     1981 atggctccat cgagaagcgc gacgccgcgg cggcgcgccg ccgggaaatg cagacgatgc
     2041 tgcgcagcgt gctcgtgaat gaagtggagc tgaagtaggg ctccggtcca cccgggcggg
     2101 ttccgagggc gccggttctc agggcgcctt cgggcccgcc tcctcgaagg cgtactcgtg
     2161 ctgggcgtcg cgcatgacga gccggcgcct gccgtccttg tccgcgacga cgaactcgaa
     2221 gcccgagatc gtcgggtcga tcgtgatgaa ggagacggtc ccgtcgtcgt tcttccgcga
     2281 ggcgaccgtg ctcttccact caccgacgtc gaagatcgtc gcggtcccct ttctcgtcac
     2341 ggaaacgtcg ccgagggccg tcgagacgta acgcggtgcg agccgcgcca ccgcggccgc
     2401 atctgccggc acgacgaggc gctcgcgctc cttcttgatc gaggccttcc gcgctgcggc
     2461 tccggccttg atctgcgcgt cggcttccgg cttgccgtcg aatagaagct cgacgagctt
     2521 tctcagaaac ggcccgcgaa ggctcacgcc ggcgtccgag ttcgcgagga tgacgccgcc
     2581 gatcccgtaa tcggggagcc agaacatgtc gctgtggtac ccggcgaggt cgccgccgtg
     2641 atgaacgatc gtgatccccc atcgggtgtc cacgaagagg cccatcccgt aggtgacgtc
     2701 ctcgctcacg aggacctggg gcgcgcggcg cgcgaggagg ttttcttccg aaacgaggcg
     2761 cgttccgtcg gggaggaggc ccttggcgag ttccatctgg acgtatcgcg agaggtcccg
     2821 cgggctcgtc cagacgccgc cggccgggcg caccggcacc accgactcgt tgagatcgat
     2881 ccgcgcgcgc acggtgttgc cgtccacgtc gtcgccgtgc ggctgcgcaa cgattcccga
     2941 gagggccttc gcgaagtcga acgtcgtgtt cgtcatcccc agcgggagga acaccctctt
     3001 ccgcatcgcc tcgtcgtacg cggtgccgag ctccttcccc ggcatctcga gcgaggccgc
     3061 gatgaagccc gccgccgccg ccatgaggtt gctgtactgg aacacctcgc cgaacttgct
     3121 cgtcggctgc atcgtgccga ggagcgtcat cgcggaggcg gggctggcgt tccggtactc
     3181 gaacagccac tcgaggtcct gtcgcggcat gcccgtgcac gcgcagacga ggtgcttcac
     3241 gaggacctgc ttcgtcgtgg ccgcgtcgcc gaggcggaac gcggggtaga cctccgtcac
     3301 gggctgatcc caacgcatct tcttcgcgtc cacctgttcg gcgacgagca gggtggagag
     3361 cgccttggta ttcgaggccg cgatgaagag cgtgtccgcg tccacaggcg ccggcttgcc
     3421 gaggtcgcgg acgccgaggc cgccctcgaa gacgactttc ccgccgtcga tgaggctgaa
     3481 cccgactccc ggcacgtcga actgcttcat cccgtccgcg aggaaggcct tcagtgccgc
     3541 gaccatcttc gcgtcgaccg gatgcgcctt cttgcccgcg aacgactcct tcgcgtagcc
     3601 cttcgggcgc aggctgttca gggcgaggga gaacggcccg ccccgtttct cgaacgtcgc
     3661 gtccgacgcg tcgacgatca ccaccgtcca cgccttgccc gcgcggaacg cgcccgcgaa
     3721 gaccgtcttc ttctcgttgg gcgaggtctc gtactggtag atgcgccgct cttcccagcc
     3781 gtttcgcgag gcctgaggcg tcgagagctt gagaggccac ttcgggcccc cggcgtacgc
     3841 ggcccaggcc gcggcgacgg cggcgtcggc gtccttcgcc tccgagtcga cgagcgcgag
     3901 gctcgagtcc ccttcgggcg gccgcaggac gacgagcggg cccttcacgg ccgcggtcca
     3961 gccggccggg gccgtgaacg tcgcgccggc cggagtcgtg cggggcgtgt ccttctcgac
     4021 gcgctcggcg gagacggggg ccgcagggag ggcctccggg ccgggcgaga aagcgagagc
     4081 gagggcgagc agcacgcggt gggtcatgag gagtccccct tcttcaggct cgggcgccga
     4141 tgggcgtacg cagattccga gaggagggtt ctcagcgctt ccgccagccc ttcgccgggc
     4201 cggcgtggaa gaacttctcc ggcgtcatga cgccgccctg cttgaagacg agcccgtcct
     4261 tcatgacgaa ccgcacgtcc cgcagcgtgt cgatgtcctc gagcgggttg cccctcacgg
     4321 cgatgaagtc cgccgcgagg ccgggcctga tcgcgccgcg ctccttttcg acctgcgcgc
     4381 acgcgtaacc cgtcgtcgtc atcgccttca ggatgtccgg cgcggggatc cccgcggcct
     4441 tccacgtgag gagaaagttg atcgtgagct cgccgcgatt catggggttg ccggtcttgt
     4501 aatagtccat gtccgtcgag aacgtgacct tgacgccgtt cttccacgcg ctcctgagct
     4561 gcgcggcgtt cttctccatg ccgtccttcg tgccgtggta cggcgtgaag ggcgtgtccg
     4621 tcgaggcgag gaagatgccc ttctgcgcca tcgccttgtg gtgcgcctcg gtgagcgcca
     4681 tcccgtgttc gatcgagaag atcccggcct cgatcgcgcg cgtcgcgccc tcgggcgtct
     4741 ggacgtggcc cgccaccttg agcccgccct tggccgcctc ggagacgaag agcttcatct
     4801 cgtccacggt gtagccccac ggcttgcagt ccacgcagat cttgatgacc ttcgcgccga
     4861 acagcatgtt ctcgcgcacg gccttcacga tctcgtcggg cgtgtcggcc tcgaggtact
     4921 cggggtagac gattccgtga tgcttcgcca tctcgggcgt cggccagaac tgcccgccct
     4981 tgccgccgat gatgaggccc gagttgacca tcgtcgggcc ggggatccag ccctggtcga
     5041 tcgcgacgcg gtgcgccgtg tcggtgtaga gcgcgttgtt cccgaggtcg cgcacgaccg
     5101 tgaagccgga gttgaggagc tggattccgt tcgacgccgc ctggatcgcg cgcagcggcg
     5161 tcgagtccat cacgtacgtc aggtagtagt agtcgtgctc gggaacgtcc ttgtacgtga
     5221 ggccgaggtg cgtgtgcgtg tccacgagcc ccggcaggac ggtgaggtcc gacaggtcga
     5281 tcaccgtggc gcccgcgggg accgcagcgc cggcgccgac ggccttgatc ttctcgccct
     5341 cgacgaggat gacctggttc gcggagatct tcccggtttc ggggtccacg agccggcccg
     5401 ccttcacggc ggtcacggcg gtcacggcgg gctgcggcac gggagcgggc gcgggctgct
     5461 gcgcggaggc ccgcgcggcg acgacgaacg acaggacgag cgcgacgcag gttcgatggc
     5521 tcatggggtc ccctcttttg cgcggagtct agtcggtggc gtcaggtccg gcaggcggcg
     5581 atcgcgatga gccactgggc cgtgaagtag ctggcgtgga cgagggcctg agcgcccggg
     5641 aacggtttgc ggaagcggtt ccaggcgagg agagagtccg agagaacgaa gagggcggcg
     5701 ccgaacgcgg tctggagagc cggagccgta tggaggttcc cggccctgct ggaggcctgg
     5761 ccggccatga cgaggaggat cgccacgtag gcggcgaccg gcaggcggag gcgccggggg
     5821 atgccgggcc agagcacacg gaagacggcg ctcccgacgg cgatccagac ggcgaacggc
     5881 agcaacgggg tggcgagagg gctgtcctgc aggaacgcga acacgtaaga ggcgtgcgcg
     5941 agcaggaaac agaagagacc cgagcggaaa cggtccgacg gaagcatgag gaagacgtcg
     6001 ccgaggaggg agaagaagag gccgccgaag accgccgccg cgtaccttga cgagaggacg
     6061 gaagacccgg cagcgacgag gagaagcaga agggccgtgc tgagcggctt gaagacgtac
     6121 acgacgggct ggcggccgag ggcgtgggcc gcgaccgtga tcgtcgccgc cgcggcgaac
     6181 gccacgctga cgaggacggg ggtcacttcg gcattcccac cgtctgcgcg aggaggatcc
     6241 gctggtcggg ccgcaggccg aggaccttcg cgagggccgg gcggtcgacg gagccgcgga
     6301 cgacggtcga gagccccgtc gcggcgcacc agaggtagac gttctggctg acgaaggccg
     6361 cgtccgccgc ggcgtagaga tcgcggtcgc cgccggaggc gcccgacatc cgcgagaggt
     6421 ccgcgacgta gacgaggttc agcggcgcgg cggtgacgaa atcctgcacg ccggtggccg
     6481 cccgaaggtc tcctttcgcg acgccgcgca gggcgtgcgc cttcgcgtcg tagacgtacg
     6541 ccccgccggg aaggacggcg tagacgtcga tctcctgcca gttcttcgcc gacggcgccg
     6601 tgcgccgccc gtcgtcgcgg ttcacgccgt ccgcggccca gagaaggtcc gacagcgcct
     6661 gcacggagag cggcgcgggc gagaactccc gggccgaccg gcgcgccttc agcgcggaga
     6721 cgagcgtggc gggagagtcg agcttcggcc tcgggagctc gagcgccggg ggaaggtcgg
     6781 ccgcgatcgc gacgccggcc gcgaagggct gaagcaggag gacgattccg aaaagccggc
     6841 gggcgcggaa gaggttcacg ggtgctcctt tcggccgccg tcggcggcga cacttcggct
     6901 tcgatcgacc ctgcttcccg atggccttac cgagacttca cggaaggttc ctttctgcaa
     6961 agcgtgccgg cgcgcgaatg ccctctcgaa agataggaag cgggagccca gcgagccagg
     7021 gggccggagg cagtcagtag gcagatcgaa taacgacggc gagaagaaga tgcacgggct
     7081 cggcctcgac gacaagggag tggtctacgt cgtcgacggc gccgtgacgc tggtcaagcg
     7141 cgtcacggtc accgacgaga ccggcaccga cagggtccgg cgcgccgccc ggtcgacgag
     7201 aagtgacacc tcgccggggg agcgccccgg tcttcagcgg gcgtatttgc ccttcgcctt
     7261 cgtgaagttc aggccgacgt gcatcgtggt gccgtgcgtc gaaaccgaca acacgaggac
     7321 gtcgctcagc gtgtagacca tgtagggctc gtcgctgtcc tcgttcttgt agaactcgac
     7381 ccagacggcg ctgaacgtgg ttccattcat gcaggcctgc cagatctttc tggacatcga
     7441 attgacgtcc atcgagaagt tcacgtcgtt cgcgcttgcc tgcctgccgg tgcctgccag
     7501 ggtgccggca gccattccga agcgcaagga cgacacctcg gtgtagtcgt cgatcggctt
     7561 gctgaactcg acgtacacgg acatgtgggc ctccccttcc gcggatccgc cactgcgact
     7621 gagtcatctt cgcacagagc cgccgtccgc ggcgggagcg ccccggccgg cctggaccgc
     7681 cgagaccttg gagaaaattg tgtccttcga ggttccactt ctaacaaaag tttgccgcga
     7741 gggtttgatt tcagcgcgta agtgactgca ttcagagccc gatgcgcttg gatcacgtct
     7801 tgcaaaaagt ctctcgagag gcgtcggccc caggccttca gcgctctctg cacgagaagg
     7861 agaagctgtg gccttgaaac gagttctcac gacatctgtt ctcttcgcat tcgcgctgac
     7921 gtccgctcat gcggcccgag ccgtcgacgc gtccgagcag ggtccgtcga cctggtcggc
     7981 tcccgccttc ttcgcgccgc ctgccgccgc gcactccgcg ggcggccgcc agtcgctcgc
     8041 ggcggggccg ggcgtcccgc ttccgttcgt cgccgtcacg ccgtgccgcc tcgtcgacac
     8101 gcgcgggaac ggcgcccccc tgcagggcgg attcctgccg gccgcgacgg tgaggacgta
     8161 cacgctcgtc ggcgtctgca acatcccggc ggacgcgaag gcgatctcgc tgaacgcgac
     8221 ggccgtcaac ccggtcggcc cgggcttcct gacgctctat gccgcaggcg cgtcgctgcc
     8281 gaccgtctcc acgctcaact tcgtcgccaa caccgtcgtc gcgaacgcgg ccgtggtgcc
     8341 gctgagcgcg tctggagcga tctcggtcgt cttcggcgtg tcgggcggcg acctcgttct
     8401 cgatacgaac ggctattact cgccgctcgg cgtcgtcaac agcctcaacg gccagggcgg
     8461 ggatctgact ctcgtcgcgg gcagcaacat cacgctcact cccggcgcgg gaacgctgtc
     8521 gatcagcgcc gcgagcggta cggggcccca gggccccgcc ggcccgacgg gtgcgacggg
     8581 ccccacgggc ccgcagggtc cgaccggcgc aaccggcacg ttcgacacgt cgtgcgtcgt
     8641 ctcggcgacg gactacgcgg cgctgaggga ctgtctcctc gcgcctgcga ccgtcttcga
     8701 cagcattccc tcgccgacac cgccgaacgt gccgagcgaa ggcttccagt gttgccagaa
     8761 ttccgaggtc ggcgaccaga tcacgctcgc gggcacggcg cgcaaggcgg tctcggcgac
     8821 ggttctcatg tcgagctggt ccctcctctc gacctacccc ggcatgagcg ccgcgggata
     8881 cacgcacccg atcacgctga acctctactc ggacgcgagc cacgccgcgg cgcacacgcc
     8941 cgacctcggg agcgtcacgc agaacgtcct ggtgccgtgg cggccgccgg cggatccgac
     9001 gtgtccgggg ggcacggcgt ggagggcggg caacggaagc tgctacaacg gctacgcgtt
     9061 cccggtcgtg ttcagcctca gcgggatcac gcttccgagc acgttcgtct acgggatcgc
     9121 gtacaacacg aacacgtggg gctacgtccc gatcggcgcc ggcgggccgt acgagtcgct
     9181 gaacgtcggc ctgaacagca cgcctccgat cgcggtcgga accgacgtcg acgtcgatgc
     9241 ggtgtggcgc tcgatgggca agccgtccgg gccgttcgcc ccggagacgg gctggacgcc
     9301 ctacacgccg gcggtgaagt tcaaggcgac gaactgagcg gcgggccggg ccgcggcccg
     9361 ggaactcttg atgcacctgc ctcaataggg agccgatctc cccgccgtca gaatcgcggc
     9421 ggggagatcg tgatggcccg cctcgtcctc catgaagtcg gcccgcgcga cggcctccag
     9481 gtcgagaaac agatcgtccc caccgcgacg aagcgcgcgt ggatcgagcg gctgctcgac
     9541 tcgggcgtcg acatcgtgca ggtcgggtcg ttcgtcaacc cggagaaggt cccccagatg
     9601 gcggacacgg acgagttgtt ccgtcagctc gcggcgcggc cggcggggaa gggcgtcctc
     9661 tcgggcctcg tcctgaacga gaaggggctc gaccgcgcgc ttctctgcgg cgtccctctc
     9721 gtgtgcctcg gcgtctcggc gagcgacacg cacagccgga agaacaccgg gatggacacg
     9781 accgaggcga cgcgccggat cgtcgcgacg gcgaggaagg ccgccgaggc gggacgcgcc
     9841 gtccaggtct cggtccagtc cgcgttcggc tgcgggttcg aggggccggt tcccgaggag
     9901 cgcgtgctct cgatcgtgaa ggagtacctc gcggcgggtc ttcgccggat ctcgctcgcg
     9961 gacaccgcgg ggcatgcgta tcccgaacag gtcgaacgcc tctacgccgc gatcctcgcg
    10021 ctcgcgcccg acgtcgagcc cgcctgccat ttccacgaca cgtacggcct cgggatggcg
    10081 aacgtctggg ccgcgatgcg cgcgggcgcc gtctcgttcg agtcgtcgtt cgcgggcctc
    10141 ggcggctgcc cgttcacgaa ggtcgcggcg ggaaacgtcg cgaccgaaga cctcgtcgcc
    10201 ggctggcagc gatccgggat ccggacggac gtcgacctcg cggtcctcat cgaggtcgcg
    10261 cgctccgtcg cggcgcattt cggccgcgag atgccgggac gcgtccacaa gacgggcccg
    10321 gtcccgacgg ctccgaagaa ggtggaggtc cccgcgtgac ccgaaaggcc ctcgaaggaa
    10381 tccgcgtcct cgacctcacg aacgtcctct ccggcccgta cgcgacgctc cacctcgcgc
    10441 tcctcggcgc cgaggtcgtc aagatcgaga acccggcgga cggcgacctc gcgcggaagc
    10501 tcggcaacgt cccgaagctg aacaaggagc tcatggggac gagcttcctc gcgcagaacg
    10561 cgaacaagaa atcgatcacg ctgaacctga agaagaagga agcgaaggag atcttcctca
    10621 ggctcgtcgc ggaatccgac gccgtcgtcg agaacttccg ccccggcgtc atgacccggc
    10681 tcggcgtcgg ctgggacgcg atgcgcgccg tgaacccgcg cctcgtctat tgcgcgatct
    10741 cgggcttcgg gcaggacggg ccggacgcgg acaagccggc ttacgaccag atcatccagg
    10801 gcctctccgg cacgatgtcc gtgaacggcg acgagcgcct gaacccgctg cgctgcgggt
    10861 tccccgtgtg cgacacggtc ggcggcctca cggcggcgtt cgcgatcatg acggccctct
    10921 accaccgcga gcggacggga gaggggcaga tgatcgacgt cgcgctcctc gacgcgatca
    10981 tgccgctcat gggctgggtg gccgcgaacc tcctcatcgg cggccagcag ccggtcctca
    11041 tgggaaacga caacttcacg gccgcgccga gcggcgtctt caggaccggg gacggcttca
    11101 tcaacatcgc cgcgaacaag cacgagcagt gggaggccgt ctgtgacgcg ctcggcgtgc
    11161 ccgagctcaa gacggacgcg cgcttcgccg agcgcgacac gcgcaagaag aaccgcaagg
    11221 acctgacgcc gctcctcgag gcgaagctcg cggggaagcc gacgaaggag tgggtcgcgc
    11281 tcctgaacgc gaaggacgtc ccgtcgggcg agatcctcgg cctcgacgag gcgctccacc
    11341 agccgcaggt cgcgcaccga ggaacgctca cgaccgtgga cgcgcccgga atcgggccgc
    11401 tcccgctctt caacctcccg gcgctcttcg gcgccacgcc ggccgcggtc gaggcgcctc
    11461 cgccccggct cggcgcgcac acggaagaga tcctgggtcg tctcggcctt tcagcgtccg
    11521 acctcacgaa cctgaagcag tcgggcgcaa tctgagcgcg atgaacgacg cagccccggc
    11581 gcccggacgc cgcatccgca tgccccacac cctcgtgatc gtgggaagcc tcatcgtctt
    11641 cgtcctcgtg ctctcgtggc tcgtgccctc gggcgcgtac cagacgatgg agaaggcggg
    11701 ccgccaggtg acggtccccg gcacgtacca gcgcgtcgcg aaggtcgtcc tcggcccgca
    11761 gtggctcgtc atcgcgccga tccgcggctt cctcgacggc tcgctcatca tcgcgttcct
    11821 catcctgatc ggcggcgcgt tcaacgtcat ccagacgacc ggcatcgtcg agcagggcat
    11881 tcggaagatc acggcggcgc tcgcgtcgag gccgcgcctc gagaagctca tggtcccggc
    11941 gctcatgacg atcttctcgc tcgcgggctc cgtcttcggc atgtccgaag aggtcatccc
    12001 gttcgtcctc atctgcgtcc cgctcgcgct ctcgctcggg tacgactcga tcgtcggcgt
    12061 ctcgattccc ttcctcggcg cggcggcggg gtttggcgcc gcgttcttca acccgttcac
    12121 cgtcggcatc gcgcagggac tggtcgggct gccgctctac tccggcctcg gttaccgggt
    12181 gatcacttgg ttcgtctgca cgggcgtgat cgtcggatgg gtcatggtct acgccgcgcg
    12241 cgtgaagcgc cggccggagt cgagccccgt gtacgcgctc gaccgcgcgc gcgaccgcag
    12301 cgcgttcgac gcgagcgcgc cgccggagcc ttggaccgtc cggcggatcc tcgtcgttct
    12361 cctcttcctc gcgggcatgg tcgctctcgt ctggggaatc ctcaaggcgc agtggttcat
    12421 cgaggagatc ggcgcgctgt tcctcgcgat gggcctcctc atgggcgccg tcgccggcct
    12481 caggcccggg gagatcgcga cgagcttcgt cgcgggcgcg aaggacatgg tcaacgtcac
    12541 gctcatcatc gcgtgcgggc gcgcgctcct catcatcgcg cgcgaggcgc gggtcctcga
    12601 cacgatcctc tacgcgagct cgggcgccat ctccatgctg cccaccgtcg tcgccgcgca
    12661 ggtgatgttc gtggtccagg cggtcatcaa cttcttcatc cactcgggga cggcgcaggc
    12721 cgccctcacg atgcccatca tggcgccgct cgccgacctc gtcggcctca cgcgccagac
    12781 gacggtgtac gcgtaccagc tctgcgagct catcaacccg atcctcccga cgtcggcggt
    12841 cacgatgggc gtcctcggga tggcgaagat cccgtgggag acgtgggcgc gctggttcct
    12901 gccgctgatg ctgacgctgt gcgcgctctc gttcctgctg ctggtcccgc ccgtcctgat
    12961 gcactggggg ccgttctaga tggcgtcgtt cgcgccgctt cttctcgccg cggccctggc
    13021 cgccggggag ccggctgccc tgcccgagga ccaccgcaag ctcgagaaga cgatctccta
    13081 cgcgcagatg gagacgttcc tccgctccgt cgacggcaag gggccggtcc gcgtttccgt
    13141 ggaaggccgg accgcgcagg ggcgcgccgt gtacctcgtc cacctgtcga cggccgcgcc
    13201 gggcgtcccg cccgcctgga aggttctctt ctacgcgcag cagcacggcg acgagatctc
    13261 gggcaaggac gcgctgctgt atctcgtgag ggacatcgcg gcgaagccgt cgctcctccc
    13321 gccggacgtc gacctctgga tcctgccgat gatgaatccc gacggcgcgg aggccggaac
    13381 gcggcgcaac gcctccgggg cggacctcaa ccgcgaccac ctcgcgctcg accagccgga
    13441 aacgcaggcg ctccaccgcg tggtccgccg tgtgcggccg cacgtggccg tcgactccca
    13501 cgagttcggc cgggactcga aggcatggcg ggcgaagggc tggcggaagt ggcccgacat
    13561 cacgatggac gggctgaaca acccgctctt cgaccgcggc gtcgtcgccg cggcgcggcg
    13621 gtgggtcgac gaaggcgccg ccgccgaggc ggacgccggc catcccttcc tgcgctactg
    13681 ggtgggcgag ccgccgccgg acggcgagca gcgccactcg tcgcccgacg tcgacagcgg
    13741 cctgaacgcc gtcgggatgt acggcgggct ctcgttcatc atcgaggcgg ccgcgtacga
    13801 gagcggcgcg cccgcggggg acctcgggaa ccgcgtggac gcatacctcg tcctctaccg
    13861 ctgcttcctc gcgggcggca gccgccgcgc ggaggacctc gccacgatcg agcgcgcccg
    13921 gaggcggcct cttccggcat tcctcccgac gaactacctc tgggtgaacc cggacgcgaa
    13981 ggtgacggag ttccccgtcg tcgaggcgtc gacgggccgc gtcctcaagg tcccgaccgc
    14041 gaacctgatg acggatatgg cggtcaagac gacggttccg acgccgctgg gctacgcgat
    14101 ctcgcccggg gcggcggagg tgttcaagac gctgctcgac aggcacggca ttcccttcga
    14161 gacgctcgcg gcgcctcgca ccgtgaaggc cgagaccgcg acgctgctcc gcgtcgagga
    14221 cacgttcgac gaggtctaca gccgctacgc ggggcgccag atcgtccgcc gcggcgaggc
    14281 tcacgcggcc gagctcgcgg ccgggagtct ctggattccg ctcgaaggcg aggccggcgt
    14341 gcgggccgcg ctcgtcctcg agccggccgc gctctacggc ctctacgagc atccgaagtt
    14401 ccgggcgctc gccggcaagg acgggacgct gccggtcctg cgcgtcgtcc gtccgcaggc
    14461 cgcgaacccg tgacgcggat cccggcggcg ctcgacgcgt tgctcgcgga gaaggtcccg
    14521 gcggtcgacc gcgccgatct cgtcctcgtg cggctcccgt tcgtgagccc gttcgcgatc
    14581 tcgacggccg tctggacgac gaaggacgcg ctgctgcttc ggctcgagtc cggcgccgtc
    14641 gcgggatggg gcgagtgcgt cgccgatccc gacccgttct acgcgtacga gacgacgggc
    14701 acggcccggc acatcctcaa ggacttcctc ctgccgcggc tcgagcccgg gatcacgctc
    14761 ggcgagctcg agcgccggat gcgtcacgtg cgcggacacg agatggcgcg cgcgacgctc
    14821 gagaacgccg ttctcgatct cgtcgcgaaa gcgaaaggcg tcccgctcca cgagctgctc
    14881 ggggcgccgc ggaagaggat cccgtccggc atcagcatcg gcctccagga atcgccggcc
    14941 gccctcgtcg cggccgtggc ggacgcggtc gcgaagaagt accaccgcgt caagatgaag
    15001 gtgcagaagg gcaaggacct cgactacgtt cgcgcggtgc gcgagagatt cccggccctc
    15061 ccgctcatgg tggacgcgaa cggcgactac cggctggacg acgcggcgca ccttgcgcgc
    15121 ctcgacgcgt tcggcctcac gatgatcgag cagccgctct cgtacgacga cgtctggcag
    15181 cacgcgctcc tgcagaagtc cctcgagacc ccgctctgcc tcgacgagtc gatccactct
    15241 ctcgacgacg ccgcggccgc gatcgcgctc ggcggctgcc ggatcctgaa cctcaagcag
    15301 ggccgggtgg gcggcatcct ggagtcgatg cgcatcctcg agcacgccgc ctcgcacggc
    15361 attccggtct ggtcgggcgg catggacgag acgggcatcg gccgcgcggt caacattcac
    15421 ctgcagacgc tggacggctt cacgctgccg ggggatacgt cggagacgag ccgctacttc
    15481 cacgaggacc tcgtggagcc gccggtcgtc ctcgacgccg aggggttcat cgacgtgccg
    15541 gaaggctcgg gcttcggtgc gagggtcgtg gacgagcggc tcgcgcgcct gacgttgtcg
    15601 gaagagagga tcttctgagc atgaagacca gcgcggcgac cgttgccgag ggcgctactt
    15661 cggcgcgggc tccttcacga ccttgcgcgc cgtgaggtcg aaaacctccc cgggcaggag
    15721 gacgtgcacc ttcaggccgt ggaccccgag gaggtccttc ccggccgcgt ccggctggcg
    15781 ggcgacggcc gcgcccgcgg cgtcgaagac catcacggag ctcttgccca tcacctggaa
    15841 ggtgcggtcc ggacgcaccc agatcgccgc cgtctcgtcc acgccgacgc cgaggagctc
    15901 gggatgctcg aggatcacgg agaagaggcg gttctccctc tggcgcttga cgaagtgctg
    15961 gtcgacgacg acgccgggga agaagccgag cccctcgagg acctccacgc tcttcgcgcg
    16021 gatgctcgtg aagtcgccct cgcccgtgat catgagcttg ctctggcagg ccgtgccggc
    16081 cgaggtgccg ccgacgacgg ctccccgcgc gtacgcttcg gcgatcgcct tgccgaccgg
    16141 cgtgtcgagg agcgccttcg tgatgcggac ctggtctccg cccccgaaga agacgccgcg
    16201 agccttgccg gcgagctcgg cgtagtcggc gcgggcggcg tcctcgcgcg tcttgatccg
    16261 gagcgagact gtcttcgtgc agccgtactt ctcccggaac agcctctcgt agtagtccgc
    16321 cgcgtcctcc tccgacgagg ccgtcgggat cgcgacgatc ggcgcgtccg gcccgccggc
    16381 gagctcgacg aactttcgca tcgcgtcgtc gggcttgtcg ccgccgccga tgagcacgag
    16441 gtttccggtt cgcgccgcgg gcggggccgc tgcgggcgcg ggagcctgcg tgcaggccgg
    16501 cgaggcgaag agggatgcga ggaggacggc cagcgggaga cgtgccttca cgggacctcc
    16561 gagacgaaac gggggcggcc gagagccgcc cccgcgatcc gatgggcggg aagaactaga
    16621 gcgtgaagcg cgcgccggcc cggacctgcc gcggctgttc gatcgtgtaa ggggcgccgt
    16681 agttgctcgc cttcgtccat gtctgccggg acgcccgcgc gtcgtacgtg ccccagcggt
    16741 tgtagttgtc gaggaccgtg cccttgttca gggcgttgaa gcactcgagc gagagctcca
    16801 gccggccgac gccggagaag ttcatgcccc aggcgaggcg caggtcgacg aggttccggt
    16861 cgtcgaactg ctcggagccg cgcgagacga gcgtgatgtc gcggcccgtc gaggagttgt
    16921 aattcaggcc gcggttgacg cgcacgtacg gcgtccagta ctggccggag aagtacgtgt
    16981 actgaccgct cgcgacgaac ccgaggggca ggtcgacggc gccggaaagc ttgaactccc
    17041 actcggcgta ggacagtgcg atccggccgt cgatgttcgt gaagccgttc ttgtcgcggt
    17101 actcgttcgc gtagccgctg ttgctcgcga tgttcccttt caggtcggtc cagacgacgg
    17161 aggagcgcag tgaccagcca tgggagtagc gcttctcgaa ccggaggacg agggagtcgt
    17221 agtcgcgata gctgccgttg tccgtcgtca gggcgaactt cgtgggggaa ttcaggatgt
    17281 agatcgggag gtcgccgccc gtcagcggat tgttcttggc cgtgaccatc gagtagtcgt
    17341 tgttcacgtt gaccatggcc atcatgctgc ggaaccggcg gtcgatgaag tcgaacccga
    17401 gcgcgacgtc cttgctcgtc tggtgctcga ccgtgaagat cgtctcgtcg acgtacgggt
    17461 ggttgacggt ccccatggtc gccgagatgg acgtcttcgg gtcgcagtcc gtgtagccct
    17521 tcgtgctcga ttcccagtag cagtcctggt ttgggatcgc cacgttgccc gagacctcgc
    17581 ggtcgtacat gtacgtgaac atgccggcgt ggtaccggcc ccagtgcgcc ttgaccgccg
    17641 tgcgggcgtt gccgaagacg tcccagacga tgccgatgcg cgggtcccag ttgtccacct
    17701 tgtagacgct cgtgttcccg tagcccggct ggaagcccgc gtcgtagtgc gtgtaccgga
    17761 tgcccgcgtt gaccgtcacg cggtcgagcc gcatcgagtc ctgggcgtag aagacgatgc
    17821 ccgtctgctg gccgtggagg ttgtactcgc cgtatccccg ctcgatgtag tacgggccgc
    17881 agcccgggtt cttgaagtac tcggtgagcc ctccggcgca gtccccggaa tagtcgtagt
    17941 acgtgaagcc gccgttgcgc agccactcgt cggacgccgt ggcgctctcg aagctgccgc
    18001 cgaacttgaa ggcgtgcgag tcggtcttgc cgaacagccc gtccttgtag aggctccacg
    18061 aaccgtcgag cgtgatgttg tggcggtggt cgaactggtg gatgtcctgg ttgacccacg
    18121 cgtagcccga gtcgtcgtcg atccggcccg gcaggctcgt cccgccgtag gggagatagt
    18181 cgtcccggcc gtcgtagccc gtgagcttca aggtcaggaa gttgctggag ttgacgaggt
    18241 actcggagtt cagggcaaac gtcgcgttcg ggccgtcctg cttgctggac gcctcgggca
    18301 gcgtgtacgc gctgacgccc cgccgctcct gggtcacggc gtcgtactcg cccatggccg
    18361 acatgcggag cttgtcggtc ggctgatacg tcagcttacc gaggaagcgg ggaatctttc
    18421 gatccgtgtc ggccagcgcg ccgacgggcg tcgtgacctg ctgccagtac tcggcggagg
    18481 cgaagaacca gagcttgtcc ttcgcgatcg gcccgccgat gctgagggcc gcatcctgga
    18541 acttgaacga tcccccgttg tcgaaacctt ccggggcgtt gttcgaggtc caggcttccg
    18601 gctggtagta gtactcgagc ccgccgtgga acgtgttgcc gcccgacttc gtgacgccgt
    18661 tgatgatgcc gcccgtgtag gcgccgtact cggcgttcgc gccgaggccg ctcacctgga
    18721 tctcgtccat ccactgaatg ctcggcagca gccagtagcc gccgccgccg gagtcggcga
    18781 cgttcacacc gtcgatcgtg taggcgttct ggcgggactg cgtgccgccg tacgcgagga
    18841 tcgcgctccc gttgcccgtc acgtccgcgt tcaccccggg ggcggtcttg acgatgctgt
    18901 agtagttgcg cggcaggggc tgcttctcga tgaagtcctg gccgaagttg ttggagaccg
    18961 cgttggacac gacgctgacg atcggcgcct cggcagtgac ggtgacctcc gtcttgacgg
    19021 agtccaggct cagcttcagg tcgacggcga gagcctggcc gaggccgacg cgcaggcccg
    19081 aacgctggac cgtccggaag cccgtcagcg tcgcctcgag ccgatacgtt cccggcggca
    19141 gagaagggaa gcggtagacg ccggcggagt cggtggtcgt cgtcgccttt ccgcgcacga
    19201 gcgcgtccga agacaccacc accgtgacgc ccggaacggc gagcccctgg tcatcccgga
    19261 cgacgccttg caggctgccc gtgagagcgc cttgggcgag tgccgcgccc ggcgagccga
    19321 gcagcagaac gagagccagt ccgaccgcga tcgagatctt tcgtgtcatg acctctcctt
    19381 attcttgtga agattcattc ttcgaggcgc agccgggcct cgccatgatg caaacggctc
    19441 aagcccgggg aacccggccg tgcgcaaatc cgttcccgca gacatgaggg gggggatcgg
    19501 cccgaagctc tacgttgcct gggcgacgct cgcggtcggc ggctcgatcg cgctcgccgg
    19561 cctcacggcg gagagcgtga cgcgcctcct cgtcctcgga ttcctcggcg cccagctcgt
    19621 ctttcgcgag agcctcgcgg cggcgctgcc ttctctcgct ccaagggctc gattcgtggt
    19681 gatgggcacg cttctcgccg ccgtcgtgga gggcttccac atgatctcga cgcccgtgtt
    19741 cccctcgctg cgcgtcgggc gcgggacgcc cccgctccag gccctggaat ccttcgcgct
    19801 ggacctcctc ttcacggtgc cggcctatct cgtgatcttc tccgtgatct gctttttcgt
    19861 ctcgcggtac cggtacggca cgtgggcgta cgtcgtcgtg atgggcttcg cgcaggcgat
    19921 cggcgacggc ggcctgttct tcttcgcgag cgcgcccgcg atgctcgtct tcctcccgta
    19981 tccgatgacg aactaccacg ccgtcaacgt gctgccgttc ctcgccgtgc ggggccagct
    20041 ggatccggag cggccgtcga gcgcccggtc ctggctcgtc gtgccggcgg tcgtggggac
    20101 ttacctcgtc tgcggagcgc tcatcaaggt cgcgggccgc tggtcgggac tggagccgcg
    20161 gtgagcggcg tcgcacggac gatcagccgc tggggcgccg agccgacgta gtagaacggg
    20221 tagcacgtca cgagcgtgac tgcggcgccg ccggtctcgt cgagaacgga cacgtcgtcg
    20281 ggatcgacga tcttcgtcca cgccaccgcg taccgcgtcg tctcgcgcgg cgtctcgaga
    20341 acgacctcgt cgccggccgc aacgtccttg agcgcccgga agaacccgtc gcggtgagcc
    20401 gagaggccga cgttccccgt ctcgccgggt cgcgacgtgc cttcgatcca gccggctccg
    20461 cggttgagcg tcaggtcgtc cgtcccctcg aggaccggga cgtcgatctt caggcgcggc
    20521 atcttcagga tcgcgagcgg cgtgcggcgg tcgaccgcga ggctctcttt ccactctttc
    20581 acgcgaacct ccgaccagag cgacacatcc accgggccct ccgcgagcgt gcgcgcgggc
    20641 gggacgctcg tgggcgtcgg gggcgccgcc ggcgcccgcg cg
//
LOCUS       DAOMFY010000039        20678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_821_length_20678_cov_10.363720, whole genome shotgun sequence.
ACCESSION   DAOMFY010000039 DAOMFY010000000
VERSION     DAOMFY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_821_length_20678_cov_10.363720"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLB01_05305"
     CDS             <1..330
                     /locus_tag="PLB01_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQQ76750.1"
                     /translation="PTFRVGERQFAMFADDHHGVGFAGVWLKAPEGAQEELVASDRER
                     FYVPPYVGKGGWVGVKLDGRTDWKLLAGLLEEAYRGAAAGQSAAKIARGRAAPKPAET
                     RPRRPRR"
     gene            339..1670
                     /locus_tag="PLB01_05310"
     CDS             339..1670
                     /locus_tag="PLB01_05310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQQ76751.1"
                     /translation="MPDRPIADGAFFRALFAAAADATVVVDGSGTIVIANPACGPLFG
                     HEPAALVGRSVETLLPARFASHARQREYFALNPLPRPMGRGLQLFALHADGKEIPVDI
                     SLSPFTVEGRLFVSCALRDQRGTSRGPDALRVQATALRSAANGVVITDRDGTITWVNP
                     AACAITGYSEDELVGRHTRILKSGRHEPSFYARLWKTVTSGESWSGTIVNRRKDGTEY
                     QEEQTIAPVLDETGSITHFIAIKQDVTDQRRTQAELGRKVEEIENLNAQLREQTVRDP
                     LTGLHNRRYLEQTMKRDISRARRTRESIVVAVIDVDHFKSVNDTHGHAVGDRILIRLA
                     DVLLGHVRSSDLVCRVGGEEFVVVMSGASVTGALKRAEIWRFSFAESVVDGRDGMPAR
                     ATISIGIARYHDPRETFAACLKRADEALYEAKRAGRDRVAVAEGDSPGNAA"
     gene            1839..2333
                     /locus_tag="PLB01_05315"
     CDS             1839..2333
                     /locus_tag="PLB01_05315"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQQ76752.1"
                     /translation="MTLLLKNLLFTVLVPGTAAVIVPLLIARGRSPASAWAAAFAVPA
                     FAAGGAVYAWCVWDFASFGRGTPLPIDAPKRLVVRGLYRYTRNPMYLGVLTVISGWAL
                     LYQIGALAGYLLFVGVVFHLFVVFYEERHLQRTFGNEYAAYRRRVGRWLPGFGRRPPG
                     SVPP"
     gene            2330..2839
                     /locus_tag="PLB01_05320"
     CDS             2330..2839
                     /locus_tag="PLB01_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76753.1"
                     /translation="MTATPELPVVESTDTLEIESPMLVGKRILFFLLGLFPLIAPYEL
                     LLEPRWTGYANVFFLFMALVSLGALCVSALLVWAAVAGIASRMTFDRGRRLFTYTAWA
                     PVMGTRTSRCPLDAIAAVEVQTHEWSDGAPSYSLNVVMKDERTFRTSSSWSRKEVETL
                     RRRVEAFLV"
     gene            2849..3151
                     /locus_tag="PLB01_05325"
     CDS             2849..3151
                     /locus_tag="PLB01_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76754.1"
                     /translation="MSDKPKTAFDLAMERLNAEDREAGAKKEAPLSAKQKEAIAEARR
                     VASSRLAEREILFRDSMRKTPDPEMRAKAEAEYLVDRQRINDDCERAIEGIRKGTS"
     gene            3209..3538
                     /locus_tag="PLB01_05330"
     CDS             3209..3538
                     /locus_tag="PLB01_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HQQ76755.1"
                     /translation="MAVSSPFGSQTRTRLLLSLELLGQSYARELARLLGASLSVVQKG
                     LGSLERDGLIAGRIVGRTRLVQVNPQYFAATQLRALIARLLAADRDLKARAASLRRRP
                     RRSGKRL"
     gene            3535..4122
                     /locus_tag="PLB01_05335"
     CDS             3535..4122
                     /locus_tag="PLB01_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76756.1"
                     /translation="MKLSRRSTLRQVAAAVAGALHDAGISAVLTGGACATIYSEGAYQ
                     SEDLDLILLSSPTQKALDQAMAELGFSRRRDFYEHPSSSFFVEFPRGPLSIGSDLSVK
                     PAEMLIDGVRVTLLSATDSCRDRLAAFYHWRDRQGLQAAVAIAARHDVDLERIRAWSE
                     REAESTNFEEFRRELRIAKASRRRLRGVRSAKRSR"
     gene            4161..5558
                     /locus_tag="PLB01_05340"
     CDS             4161..5558
                     /locus_tag="PLB01_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HQQ76757.1"
                     /translation="MKRFLPWMVAVAFLMESLDTTILNTAVPTIADALGVVPLSMKSV
                     LSSYTLSLAVFIPVSGWIADRFGTRRVFATAIGIFTLGSLLCGLATSIHVLVACRILQ
                     GMGGALMVPVGRLTIVRTFERSELVKAMTFVTIPALIGPMLGPVAGGFIVGYLHWRVI
                     FFVNLPIGLLGLALVLRRFPDYRSESVPRLDVLGGVLFGAGIGLLSYILEVFGEHTLS
                     TGAVLGLFALAVTLLAAYFRHAMAAAHPLLKLGLFRLRTFRSAVMGSFITRLGAGGIP
                     FLLPLLYQVGLGYTAVQSAFLLMPQSIAAMGIRTAMPRILERFGYRRVLLTNTVLIGV
                     AIALFATVGTGTPVALIVVFSLLFGAAASLQYASMNTLGYADIAAPDASMAATISSTM
                     QQMSLSFGVATASLVTALFIPDRFRSHPPELLHGIHLALLVLGAMTVLSAVVFRELKP
                     EDGDNVSQHGEAAGE"
     gene            5599..8340
                     /locus_tag="PLB01_05345"
     CDS             5599..8340
                     /locus_tag="PLB01_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76758.1"
                     /translation="MTIPSGTRLGPYEILAPLGAGGMGEVYRARDPRLAREVAIKVLP
                     EEFFEGEERRLRFEREAKLLASLNHPGIAAIYSFEEIPSSSSSSSRHLLVMELVEGED
                     LAQRISAGPLPLEESLSCARQIAEALEAAHEKGIVHRDLKPANVKVTPDGKVKLLDFG
                     LAKIFEGDPGRPGSGAGVTASPTLTARGTGAGVILGTAAYMSPEQARGKPVDKRTDVW
                     AFGCVLYEMLAGKRAFDGETVSDALAAVLMKEPDWSALPPGTPERVKEILRKCLRRDA
                     KLRLRDIGDARLDLEDLSAAAASSSSAQFTFEEKSTVPGPTAAGAERASRERGPRKLL
                     SLYLPWALAAAFAAVAGWALLRGRPPAFQARALKVAVVPPAGTISSGPIDISPDGSQI
                     AFTSAGPDGHTKLYVRSLDSLEPKVLPGTDDADAPFFAPDGRSIGFFAAGKLKRVDLA
                     GGPARELADAPDHRGGSWGSQNLIVFAPEGGGTIFSIPASGGAAKPVTTFDPATAETS
                     HRWPRFLPDGKRFLFMSRRPKPPGRLMVEAGSVDGGKRTRLVESSTGGVYARGRLYFV
                     REETVLSQAFDLRTLAVSGEPAPAAENVWRNLNTDGLTAFSVAEDGTLAFRRGGILKS
                     QLTWIDRQGRPLGTVGAPSVLGDMDLSRDDRRVLVDITDPVRDVSAVYVIDAAAATTR
                     VTLGAGNQSAGLFSPDEKSFVFASDVKGAFDLYSREVGSGAEPTPLLVNSVWKFPEHF
                     SPDGRFLSYTQSESGKPRDIWILPLKGGGAPFPFAQTPAEEWGSVFSPDGRFIAYVSD
                     ESGRSEVYVRTFPSSAAKWQVSTSGGTSPVFRRDAKELFYLAPDRTLVAVPITLAQGV
                     LTAGTAKPLFRNSSLHLASVSGNTPYGVSADGQRILAIVTVGEAEASWIVVQTVAGR"
     gene            8474..9520
                     /locus_tag="PLB01_05350"
     CDS             8474..9520
                     /locus_tag="PLB01_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76759.1"
                     /translation="MTFRRLCRSLALLLLLRPPAAPAVEALTTTVEEVNARKKIPRLK
                     EREAVLTRQRDAAKATLDEIARDPDVIAAFDEMKVGKPGATMLTPDDKRFKSLDAEKK
                     KSVDRFQEAEAAFAFYQNSLKQTQAQTHDLQAIPISANVQINSGGSASAVVPGIDASG
                     SFPISNSLGCQMLFSIKPDPPAGTTADVAQSIRMSTGVFSANLGLNYANFRAESDTLN
                     GPLGVEARLGLPVAYQRAASTDPPASGQASTSSRDFGLLSPEIKVSVWLKYVLLGYKY
                     VYYFAFGNPNPVYEEIDRTGSHRIYLAAKLQALSGSDKTPFYVEANYVSGKNGFSGGA
                     FSFAVSKSLSWDPR"
     gene            complement(9538..11613)
                     /locus_tag="PLB01_05355"
     CDS             complement(9538..11613)
                     /locus_tag="PLB01_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQQ76760.1"
                     /translation="MKAFTKWLGALALAISTTAVAQTGDDPFRWLEDVQGEKALAWVK
                     EHNAKSTALLTARKEYKPIYAKTLEILDSKEKIPSPELHGETVYNFWKDDAHERGVWR
                     RTSLASYRTASPEWETVLDVDALAKADGKAWVWHGAVCLPPANVRCLVRLSPGGSDAA
                     VVREFDTASKQFVAGGFTLPEAKHRVSWRDEDTLWVGTDFGPGSRTASGYPRFVKLWK
                     RGTPLTEAKTVFEGRTEDVSVDGTSEILSDGRYDLVTRTPAFFRIEAFLYLGGRLVKI
                     DMPEDARPRGFFRDRMLISLRSDWTTGGKTYRAGSLIAGTVDDVLRGVRRFDVLFEPG
                     PRVSLGRVDRTKDRVLIQTLDNVRSRITALALEDGAWKRSEVALPGLGTASFASADDL
                     TNAYFFTYQDYSSPTSLWLTGTSEPVKVKSMPAFFDATGVKTEQFEAVSKDGTKIPYF
                     VVTPKGFKADGTAPTLLYGYGGFEQSEVPRYSGTIGAAWLARGGVYVDANIRGGGEFG
                     PEWHKAAVKEKHIHNFEDFSAVASDLIARKITSARHLGIMGGSQGGLLVGGTFTLYPD
                     LFRAVVAQVPLADMRRYNKLLAGASWMAEYGDPDKPEDWAYIKTWSPYELLTKDAKYP
                     TPFYWTNTKDDRVHPGHARKMVAKMESLGHPVYYFENTEGGHGSGAVNKQTAEVTALQ
                     YAYLWMMLR"
     gene            complement(11947..12474)
                     /locus_tag="PLB01_05360"
     CDS             complement(11947..12474)
                     /locus_tag="PLB01_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76761.1"
                     /translation="MKFEFFESLSRAEAEKYLQNFLEVEGKAFEGLAVEAAADGLLLN
                     FTLPSLAPLYEWVATRAAVVSKEPDPALPPWIRNTESYAEGLFDLTPETSAAVMRAAY
                     YLGETFRRQFPSLHWGIGDPSTAEQQQPVVGPFQHGIEMSVILVTENLVRGMIKGKGR
                     EIAERAFARWASSVN"
     gene            complement(12471..12995)
                     /locus_tag="PLB01_05365"
     CDS             complement(12471..12995)
                     /locus_tag="PLB01_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQQ76762.1"
                     /translation="MGLIKGCTFERTTFSEVAFGGAQIIESRFVACEFGRVDARDLMF
                     RQTHIQGLVLSGTVSSCNLVDCTVSSLDASSAYVDDLGVVGGSLRDVTWPSHDSNFAV
                     APSSLIEVMAEVVTKLRPESRARYEEMARLYSVSTSPVVMIARSGFRGMTDVEAAAVM
                     KALRGLEISNTSHP"
     gene            13390..13860
                     /locus_tag="PLB01_05370"
     CDS             13390..13860
                     /locus_tag="PLB01_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQQ76763.1"
                     /translation="MDFELTKRVLGEFERHHVDYVVFGAVAVNLQGLARATEDLDVFI
                     APRVENIERLKAALRAVFDDPNIDQITADDLLGEYPAVQYIPPQGGFHIDILTRLGEA
                     FRFEDLASEKIDLGGVSVTVATPTTLYRMKKGTVRPKDWGDAEALRVRFRLPEE"
     gene            13857..14102
                     /locus_tag="PLB01_05375"
     CDS             13857..14102
                     /locus_tag="PLB01_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76764.1"
                     /translation="MMPVRRFRDVSEMEGTLWRKPGDPELFRAIAGVWDFAARTVPRH
                     FPPGVYRHRSIEEAEALREKWEEADFQALHEDRRRKG"
     gene            complement(14131..14862)
                     /locus_tag="PLB01_05380"
     CDS             complement(14131..14862)
                     /locus_tag="PLB01_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76765.1"
                     /translation="MASVSVSKGFLVGIAAALGAALLALAYQLGKASGSGEVPAPPAR
                     IERIAPSPKEEPLPRPARIAGATTDYGDTRAVSAPTPSAPEQPAAPQAAAGQVPEPTE
                     GERGGGDISMRAAVAAYFFAVERIQVGSMTGNAESVGQEMAAALANGDTSSLEKMIRE
                     TEAARVRLAALSPPAPCVAHHRESLGSLDDALEVLRSLKTAMESPEPIGQLANVTTRA
                     QALRSRADVLQKEERALKERYGLMR"
     gene            14987..15241
                     /locus_tag="PLB01_05385"
     CDS             14987..15241
                     /locus_tag="PLB01_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQQ76766.1"
                     /translation="MNPRKLLQKALASPNNFRFAEARALAEAFGFRLNRVSGSHHIFV
                     HSEVPQLLNLQEVHGQAKPYQIRQLLQLIERNNLKLAGDE"
     gene            15238..15456
                     /locus_tag="PLB01_05390"
     CDS             15238..15456
                     /locus_tag="PLB01_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQQ76767.1"
                     /translation="MKDYHINIFQSDEDGGYIADIPDLPHCSAFGETPEKALEEVLKA
                     KAAWLDAAREAGKPIPPPSFKPAIYQVA"
     gene            complement(15498..17285)
                     /locus_tag="PLB01_05395"
     CDS             complement(15498..17285)
                     /locus_tag="PLB01_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76768.1"
                     /translation="MRPIDKRTLVAQWAFDTRPVLLRFHLWLEDVELERSQPEPVSDH
                     SFTPRGIARCLAMTSAATALGTRLFGGYGEGAGADKATLNQAKKAADAISAYVMSEGL
                     WHLTRTLPENHAIMVCLGEGLMPKAGETAEMGANPLLGFGRVYARPEVARVVDRRVRR
                     LLNESGHTFEHFHDWLKSRGITIWGAAVDTLENTVRFAEGKATGPMSVFHLFDSPLIL
                     ARPYESYMGCLTVPRRVAEAADRASLLLDYRTPRADVTRAIEAAYPGVKRGNIHVWTL
                     RGKNRVARLGELWEEWKALGVHLVEDGWKAPSGLEVFTDSGTYAPTFLIGSWADKAGE
                     THVFLCDGYAATAEAMQAASLADVLDVDASMSLFSPGFDFPCVAEGRLMALDPDKPGF
                     AQRVREVLGGRDVEAGRVVSYAEAIREAKACNVPTGRPALKPGDFVPAKNWRVLASTG
                     YICDDPYTGTAGVTKVGDGVYKVTTKLAAHQASSLITFTFRLMEPLETARHVFSPLLV
                     RFMSGVDYTTRPVKISDSGRIRNELQTMFSQALEHDGDTIRIHFDRIDDKVVPRDKQK
                     TIRKVLEWYRENHPAWFSWLEIGGTTPRI"
     gene            complement(17399..17767)
                     /locus_tag="PLB01_05400"
     CDS             complement(17399..17767)
                     /locus_tag="PLB01_05400"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQQ76769.1"
                     /translation="MRKCEKEKYARYRQTRYSPVAGPSRPAKARFHPEAQAEYLEVIA
                     YLGLHAPSAVDAFAGDVQEAVDFIEKYPEGAPVERGSVRCKLLRRFPYELYYSPEADF
                     VRILAVAHQRRRPGYWVDRE"
     gene            complement(17685..17912)
                     /locus_tag="PLB01_05405"
     CDS             complement(17685..17912)
                     /locus_tag="PLB01_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HQQ76770.1"
                     /translation="MTREDLLAAALKLPLDERAALADRLLRSLDELPEPERKTLWLEV
                     AERRLAEMREGKVREIPADEVFARGRAVTSR"
     gene            complement(17950..18300)
                     /locus_tag="PLB01_05410"
     CDS             complement(17950..18300)
                     /locus_tag="PLB01_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76771.1"
                     /translation="MWLITTSGFLSVVRNLDSTSPDDALLVRGRVRADLERFADFAAQ
                     RGGRPAVVESPEADYGFRLTTSRDVLAAFVAERVAALDYPNFKSEIYKADPVREKVYE
                     DAWAVLHGLKKVRA"
     gene            complement(18357..18875)
                     /locus_tag="PLB01_05415"
     CDS             complement(18357..18875)
                     /locus_tag="PLB01_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76772.1"
                     /translation="MSLHHKAFVFDYRRFNVELAPALLRALEIRSSDELRRLINSNLE
                     SFRDPYEGEPLTRDWEARLEFGNEHEVGDFALTKFYSPVLNLGLGSDWMDVDRALVSL
                     GEGLGLTLLGTPFGPPENPFDPGRTGSYFQSPEDVVRNLSDLRRARRTTAVERAEAIL
                     KKAVGYGLYATF"
     gene            complement(18904..19350)
                     /locus_tag="PLB01_05420"
     CDS             complement(18904..19350)
                     /locus_tag="PLB01_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HQQ76773.1"
                     /translation="MKRTGESMTEWRERIHGKLVAPAFQRADDFPAAEASSRLGVPLP
                     EAFTEFFRSYVGPFGSKSSGFELLDICEGHPTIVSSTIECRGRFGWPERLVVLTDLLG
                     NSVLVLDGATGAVYNVDFEGGERLIVEGRLPPSWNSFEAFLEDFFR"
     gene            complement(19334..>20678)
                     /locus_tag="PLB01_05425"
     CDS             complement(19334..>20678)
                     /locus_tag="PLB01_05425"
                     /inference="COORDINATES: protein motif:HMM:NF025768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQQ76774.1"
                     /translation="GSATIAPSAHGGSVNEIGLRPLGGWERGATYRVRVTDAIRDVRG
                     QPLTNAEDFSFTVPKSGPFETRHEPRRPVAQANVQAASAGPAFPGGQALGWHGHWTDP
                     VTGLIYMRNRWYDPRTGTFLSPDPLEDIDSPNQYLFGAGRPHEVVDPLGLAVSTSKRG
                     IVISDERNNGYWYLIPWALAYANPASTVELLRRGGLAGSALDEFMESNGINLGTDASR
                     AQALGLMTSQELARAQRFTIHAETEIATAYGAGKVLGLVGRGIASTVLKRAAQEGSES
                     GYRLLARELWPSYPETPVMFPEGVGPAQASGRAGSATGSVSFPSSVRFTPSTGRTYTV
                     YQRADIDWSQVRTAGPRDFIGKTNAEAAKAGFRPQLPDGEFATLHHAEQSATGPWFEA
                     STRYHNIRTAQQPPLHPYEGGQNPYAPLGRGPGSPRDAFQTIESPEYWQWREAHR"
BASE COUNT         3328 a         7250 c         6625 g         3475 t
ORIGIN      
        1 cccacgttcc gcgtgggcga gcgccagttc gcgatgttcg cggacgacca ccacggcgtc
       61 ggcttcgccg gcgtgtggct gaaggcgccc gagggcgcgc aggaggagct cgtcgcctcc
      121 gaccgcgagc gcttctacgt cccgccgtac gtcggcaagg gcggctgggt gggcgtgaag
      181 ctggacggca ggacggactg gaagctcctc gcgggcctcc tcgaggaggc ctaccgcggc
      241 gccgccgccg gccagagcgc cgcgaagatc gcgcgcgggc gggccgctcc gaaacccgca
      301 gaaacgcgac cgagacgccc gcgacgctaa gatccggtat gcccgatcgc ccgatcgccg
      361 acggcgcatt ctttcgcgcg ctcttcgcgg ccgccgcgga cgcgaccgtg gtcgtcgacg
      421 gttccggcac gatcgtcatc gcgaatccgg cgtgcgggcc cctcttcggg cacgagccgg
      481 ccgcgctcgt cggcaggtcc gtcgagacgc tcctgccggc gcgcttcgcg tctcatgcgc
      541 ggcagcgcga gtatttcgcc ctgaatcccc ttccgcgtcc catggggcgc ggcctccagc
      601 tcttcgcgct ccacgccgac ggcaaggaga tccccgtcga catctcgctg agccccttca
      661 cggtggaggg ccgcctcttc gtgtcctgcg ccctccgcga ccagcgcggc acttcgcgcg
      721 gacccgacgc gctccgcgtc caggcgacgg cgctgcgctc cgccgcgaac ggcgtcgtca
      781 tcaccgaccg cgatggcacg atcacgtggg tcaacccggc ggcgtgcgcc atcacgggct
      841 actccgagga cgagctcgtc ggccggcaca cgcggatcct gaagtccggc cgccacgagc
      901 cctctttcta cgccaggctc tggaagaccg tgacgagcgg cgagtcgtgg tccggcacga
      961 tcgtgaacag gcgcaaggac ggcacggagt accaggaaga gcagacgatc gcgcccgtcc
     1021 tcgacgaaac cggctccatc acgcacttca tcgcgatcaa gcaggacgtc accgaccagc
     1081 gccgcacgca ggcggagctc gggcgaaagg tcgaggagat cgagaacctg aacgcccagc
     1141 tccgcgagca gaccgtgcgc gatccgctca caggcctcca caaccgccgc tacctcgagc
     1201 agacgatgaa gcgcgacatc tcgcgggccc ggcggacgcg ggagtccatc gtcgtcgccg
     1261 tcatcgacgt cgaccacttc aagtccgtca acgacacgca cggccacgcc gtgggcgacc
     1321 gcatcctcat ccggctggcg gacgtcctcc tcggccacgt gcgttcctcc gacctcgtct
     1381 gccgcgtcgg cggcgaggag ttcgtcgtcg tcatgtccgg cgcgagcgtt acgggcgcgc
     1441 tgaagcgcgc ggagatctgg aggttctcgt ttgccgagtc cgtcgtcgac ggccgcgacg
     1501 ggatgcccgc gcgggccacg atctcgatcg gaatcgcgcg ttaccacgat cctcgggaga
     1561 cgttcgcggc ctgcctcaag cgcgccgacg aggcgctgta cgaggcgaag agggcgggcc
     1621 gggaccgcgt cgcggtcgcc gagggcgatt cgcccgggaa cgccgcctga aagaaagacg
     1681 ccggagcgtt tccaccccgg cgccggaacg tcacgtcgtg ggcgcgatcg ggagcggcgt
     1741 ccgccgccgc cactgaagcc gccgccgccc ccgcggctct cctggcgctg ggtaggccct
     1801 gtcgaaagca gtatcgtcag gtggacaaaa ggcaagccgt gacgctgctc ctcaagaacc
     1861 ttctcttcac ggtactcgtt ccgggcacgg ccgcggtcat cgttcccctg ctgatcgcgc
     1921 gaggccgctc gcccgcgtcg gcctgggccg cggcgttcgc cgtccccgcg ttcgccgccg
     1981 gcggggccgt ctacgcgtgg tgcgtgtggg acttcgcctc tttcgggcgg ggaacaccgc
     2041 ttccgatcga cgcgccgaag aggcttgtcg tgcgcgggct ctaccggtac acgcgcaatc
     2101 cgatgtacct gggcgtcctc acggtgatct cggggtgggc actgctgtac cagatcggcg
     2161 cgctggccgg ctatctcctg ttcgtcggcg tcgtctttca tctcttcgtg gtcttttacg
     2221 aggagaggca cctgcagagg acgttcggaa atgagtacgc cgcctatcgt cgacgggtcg
     2281 gtcgctggct ccccgggttt ggacggcggc cacccggttc ggttccaccg tgaccgcgac
     2341 ccccgaactc ccggtcgtcg agagcacgga taccctcgag atcgaatccc cgatgctcgt
     2401 cgggaagaga atcctcttct tcctcctcgg cctcttcccc ctgatcgcgc cgtacgagct
     2461 tctcctcgag ccgcgctgga ccggctacgc aaacgttttc tttctgttca tggcgcttgt
     2521 ctctctcggg gccctgtgcg tctcggccct cctcgtctgg gcggccgtgg ccggaatcgc
     2581 gtcgcgcatg acgttcgacc ggggccgccg gctcttcacg tacacggcct gggctccggt
     2641 catggggacg cgcacgtccc gctgcccgct cgatgcgatc gccgccgtcg aggtccagac
     2701 ccacgaatgg agcgacggcg caccgagcta ctccctgaac gtcgtgatga aggacgagcg
     2761 gacgttccgg acgtcgtcct cctggtcccg aaaggaagtg gagacgctcc ggcggcgggt
     2821 cgaggcgttt ctggtgtaga agggggccat gagcgacaaa cccaagaccg cgttcgatct
     2881 cgcgatggag cgcctgaacg ccgaggaccg cgaggcgggt gcgaagaaag aggccccgct
     2941 gagtgcgaag cagaaggaag ccatcgccga ggcgcggcgc gtcgcttcct cccgcctcgc
     3001 cgagcgcgag atcctcttcc gggactcgat gagaaagacg cccgaccccg agatgcgcgc
     3061 gaaggccgag gccgagtacc tcgtcgaccg ccagcgcatc aacgacgact gcgagcgggc
     3121 gatcgaggga atccggaagg ggacgagcta gcctcgcctc tgcttcggct cttgacatag
     3181 acctctataa gctgtcatat gacgtcatat ggcggtcagc tctcctttcg gctcgcaaac
     3241 ccggacacga cttctcctct ccctggagtt gctcgggcag agctacgcgc gggagctggc
     3301 gcgcctgctg ggcgcctctc tctcggtcgt tcagaaaggc ctggggagcc tggagcggga
     3361 tggcctcatc gcaggtcgga tcgtcgggcg gacgcgcctc gtccaggtca acccgcagta
     3421 tttcgccgcc acgcagctgc gggctctgat cgccaggctg ctcgccgccg atcgcgacct
     3481 caaggcgcga gccgccagcc tgcggcggag gcccagacgg agcggaaagc gcctgtgaag
     3541 ctctcgcgcc ggtccacgct gcggcaggtc gcagccgccg tcgccggcgc cctccacgat
     3601 gcgggcattt ctgcggttct cacgggaggg gcctgcgcga cgatctattc ggagggcgcg
     3661 tatcagtcgg aggacctcga cctgatcctc ttgtcctcac caacccagaa ggcgctcgat
     3721 caggcgatgg cggaactcgg tttctcacgg cgtcgggatt tctatgagca cccgagttct
     3781 tcgttcttcg tcgagttccc ccgggggccc ttgtccatcg gatcggacct ttcggtcaag
     3841 ccggccgaga tgctcatcga cggcgtacgc gtcaccctgc tttcggcgac ggattcctgc
     3901 agagatcgcc tcgcggcctt ctatcactgg agggaccgcc agggcctgca ggcggcggtt
     3961 gcgatcgcgg cacgacacga cgtggacctc gaacgaatcc gcgcctggag cgaaagggaa
     4021 gcggagagca cgaacttcga ggaattccgg cgggaactgc gcatcgcaaa agccagtcgg
     4081 cgccggcttc gtggcgttcg cagtgccaaa cgtagtcgat agtggctgct cctcccgtgt
     4141 cctccagccc cgatccctcc atgaagcgct tcctcccctg gatggtggcg gtggcgttcc
     4201 tgatggagtc gctcgacacg accatcctca acacggccgt ccccaccatc gccgacgcgc
     4261 tcggcgtcgt gcccctcagc atgaagtcgg tcctgtcgag ctacacgctc agcctcgccg
     4321 tcttcatccc cgtcagcggg tggatcgcgg accggttcgg cacgcggcgc gtcttcgcga
     4381 ccgcgatcgg catcttcacg ctcggctccc tcctctgcgg cctcgcgacg agcattcacg
     4441 tcctcgtcgc ctgccgcatc ctgcagggca tgggcggcgc gctcatggtg cccgtcggcc
     4501 gcctcacgat cgtgcgcacg ttcgagaggt ccgagctcgt caaggcgatg accttcgtga
     4561 cgattcccgc cctcatcggg ccgatgctgg gcccggtcgc gggcggcttc atcgtcgggt
     4621 acctgcactg gcgcgtcatc ttcttcgtca acctgccgat cggcctcctc ggcctcgccc
     4681 tcgtcctccg ccgcttcccg gactaccgct ccgagagcgt ccccaggctg gacgtcctcg
     4741 gaggcgtcct cttcggcgcc ggtatcggcc tcctgtccta catcctcgag gtcttcggcg
     4801 agcacacgct ctcgacgggc gccgtcctcg gcctcttcgc cctcgcggtc accctcctcg
     4861 ccgcctactt ccgccacgcg atggccgccg cgcacccgct cctcaagctc ggcctcttcc
     4921 gcctgcggac gttccggtcg gccgtgatgg gcagcttcat cacgcgcctc ggcgcgggcg
     4981 gcatcccgtt cctcctcccg ctcctctacc aggtcggtct cggctacacg gccgtgcagt
     5041 cggcgttcct gctcatgccg cagtccatcg ccgccatggg aatccgcacg gcgatgcccc
     5101 gcatcctcga gcgcttcgga taccggcgcg tcctcctcac gaacacggtc ctcatcggcg
     5161 tcgccatcgc gctcttcgcc acggtcggca ccggcacgcc cgtcgccctc atcgtcgtct
     5221 tctctcttct cttcggcgcc gcggcatcgc tgcagtacgc gagcatgaac acgctcggct
     5281 acgccgacat cgccgcgccg gacgccagca tggcggccac catctcgagc acgatgcagc
     5341 agatgtcgct gagcttcggc gtcgcgacgg cgtcgctcgt gacggcgctc ttcatcccgg
     5401 accggttccg gtcgcacccg cccgagctcc tccacggcat ccacctcgcg ctcctcgtcc
     5461 tcggcgcgat gaccgtcctt tcggccgtcg tgttccgcga gctgaagccg gaagacggcg
     5521 acaacgtgag ccagcacggg gaggcggccg gggagtgagt cgcaggacgc gctgcggcta
     5581 cactgccgcc cgaccttcat gacgatcccc tcgggaaccc ggctcggacc gtacgagatc
     5641 cttgccccgc tcggcgcggg cgggatggga gaagtctaca gggccaggga cccgcgcctc
     5701 gcgagagagg tcgcgatcaa ggtcctgccg gaggagttct tcgaaggtga agagaggagg
     5761 ctgcgcttcg agcgcgaggc gaagctgctc gcgagcctga atcatccggg catcgccgcc
     5821 atctactcat tcgaggaaat cccctcttcc tcttcttctt cgtcccggca tctcctcgtg
     5881 atggagctcg tcgagggcga agacctcgcc cagcggatct ccgccggccc ccttcccctc
     5941 gaggaatccc tctcctgcgc ccgccagatc gccgaggcgc tcgaggcggc gcacgagaag
     6001 ggcatcgtcc accgcgacct caagcccgcg aacgtcaagg tcacgcccga cggcaaggtg
     6061 aagctgctgg acttcggtct cgcgaagatt ttcgagggag accccggaag gccgggctcg
     6121 ggggccggcg tcacggcgtc tccgacgctc acggcgcgcg gcaccggggc cggagtgatc
     6181 ctcggaaccg ccgcctacat gagccccgag caggcgcgcg gcaagcccgt cgacaagcgc
     6241 acggacgtct gggcgttcgg gtgcgtgctg tacgagatgc tcgccggaaa gagagccttc
     6301 gacggcgaga ccgtgagcga cgcgctcgcg gccgtgctca tgaaggagcc ggattggagc
     6361 gcgctgccac cggggacacc ggagagggtc aaggagattc tgagaaagtg cctgcggcgc
     6421 gacgcgaagc tgagactgcg ggacatcggc gacgcgcgcc tcgacctcga ggacttgagc
     6481 gcggcggccg cgtcgagctc ctccgcccaa ttcaccttcg aagaaaaatc gaccgttcca
     6541 ggccccacgg cggcaggcgc ggaacgagcg agccgcgaga gagggccccg gaagcttctc
     6601 tctctctatc tcccctgggc gcttgcggcc gcgttcgcgg ccgtggcggg atgggcgctc
     6661 ctgcgcgggc gtccgcccgc attccaggcg cgagcgctga aagtcgcggt cgtccctccc
     6721 gccggaacga tctcctcggg gcccatcgac atttcgccgg acggaagcca gatcgccttc
     6781 acgtcggccg gccccgatgg acacacgaag ctctacgtgc gaagcctcga ctccctcgag
     6841 ccgaaggttc ttcccggcac cgacgatgcg gacgcgccct tctttgctcc cgacgggcgc
     6901 tcgatcggct tcttcgcggc cgggaagctg aagcgcgtcg acctcgccgg cgggcccgcg
     6961 cgcgagctcg ccgacgcacc cgatcaccgc gggggaagct ggggctcgca gaacctgatc
     7021 gtcttcgccc cggagggcgg cgggacgatc ttcagcattc ccgcctcggg gggcgccgca
     7081 aagcccgtga cgacgttcga cccggccacg gcggagacct cgcaccgctg gccgcgtttc
     7141 ctgccggacg gcaagcgctt cctcttcatg agccgcaggc ccaagccgcc gggacggctc
     7201 atggtcgagg cgggctccgt cgacgggggc aagcggaccc gtctcgtcga atcttcgacc
     7261 ggcggcgtct acgcgcgcgg ccgcctctac ttcgtacgcg aggagacggt gctctcccag
     7321 gcgttcgatc tcaggacgct cgcggtctcc ggcgaaccgg cgccggcggc cgaaaacgtc
     7381 tggcgcaacc tcaacacgga cgggcttacc gcgttctcgg tggcggaaga cggaacgctc
     7441 gcgtttcgcc gtggcggaat cctgaagagc cagctcacgt ggatcgaccg ccagggacgg
     7501 ccgctcggaa ccgtcggggc gccttcggtc ctcggggaca tggacctctc gcgggacgac
     7561 cgccgcgtcc tcgtggacat cacggacccc gtgcgcgacg tcagcgctgt ctacgtgatc
     7621 gacgccgcgg cggcgacgac gcgcgtgacg ctcggcgcgg gcaaccagtc cgcgggcctc
     7681 ttctcccccg acgaaaagtc cttcgtgttc gcgtccgacg tcaagggagc gttcgacctc
     7741 tacagccggg aggtcggctc cggcgcggag cccacgcctc ttctcgtgaa cagcgtctgg
     7801 aaattcccgg agcacttctc gcccgacggg cgtttcctga gctacacgca gagcgagtcc
     7861 ggaaagccgc gggacatctg gatcctgccg ctgaagggcg gaggtgcgcc gttcccgttc
     7921 gcgcagacgc ccgccgagga gtggggatcg gtgttctcgc ccgacggtcg cttcatcgcg
     7981 tacgtctccg acgagtccgg ccgctccgag gtctacgtcc gcaccttccc ctcgtcggcg
     8041 gccaagtggc aggtctccac gagcggaggc acctcgcccg tctttcggcg ggacgcgaag
     8101 gagctcttct acctcgctcc ggaccgcacg ctcgtcgccg tcccgatcac gctcgcccaa
     8161 ggggtgctca cggccggaac cgccaagccg ctcttccgga actccagcct gcacctcgcc
     8221 tctgtctcgg gaaacacccc gtacggcgtc tccgccgacg gccaacgcat cctcgcgatc
     8281 gtcaccgtcg gcgaagcgga agcgtcatgg atcgtcgtgc agacggtggc ggggcggtga
     8341 cgtgcagagc agacgctcga ctcggtcgga actgctgcgc cgccccgggg tcaatgccgg
     8401 cggctcggct gtctaactag acagagagca gcccacgcct cgtcgcctgc cttccgttca
     8461 actggaggca accatgacgt tccgccgtct ctgccggagc cttgcccttc tcctgctcct
     8521 ccgtccgcca gccgcacccg ccgtcgaagc actcacgacg actgttgagg aagtgaacgc
     8581 aaggaagaaa attccacgcc tcaaagagcg cgaggctgtc ctgaccagac aacgggacgc
     8641 tgccaaggcc acactcgacg agatcgcgag agatccagac gtcatcgcgg cgttcgacga
     8701 gatgaaggtc ggaaagccag gcgccacgat gttgaccccc gacgacaagc gcttcaagtc
     8761 gctcgacgcc gagaagaaga aatccgtcga ccgttttcag gaggcggagg ccgctttcgc
     8821 cttctaccag aactccttga aacagacgca ggcgcagacc cacgacctcc aggcgattcc
     8881 gatctcggcc aacgtccaga tcaactccgg cggcagcgcg tccgccgtcg tgccgggcat
     8941 cgatgcgagc ggctctttcc cgatctcgaa ctcactcggc tgccagatgc tcttcagcat
     9001 caagcccgat ccgccggcag ggacgaccgc cgacgtcgcc cagtccattc gaatgtcgac
     9061 gggcgtcttc tctgccaacc tcggcctcaa ctacgccaac ttcagggccg agagcgacac
     9121 gctgaatggt cctctgggtg tcgaggcccg gctgggattg cccgtcgcct accagcgtgc
     9181 ggcctcgacg gatccgccgg cttccgggca ggcctcgacg tcgtcaaggg acttcgggct
     9241 gctgtcgccg gagatcaagg tcagcgtctg gctgaagtac gtgctgctcg gctacaagta
     9301 cgtctattac ttcgccttcg gaaacccgaa ccccgtctac gaagagatcg accggacggg
     9361 gtcgcacagg atctacctcg ctgcgaagct ccaagccttg agcggcagcg acaagacgcc
     9421 cttctacgtc gaggcgaact acgtgagcgg caagaacggc ttcagcggag gcgcattcag
     9481 cttcgccgtc agcaagagcc tctcctggga tccgagatag gccaggacgg aacgccgtca
     9541 gcgcaacatc atccagaggt acgcgtactg gagcgcggtg acctccgccg tctgcttgtt
     9601 gacggcgccg gacccgtgcc cgccctccgt gttctcgaag tagtagacgg ggtggccgag
     9661 ggactccatc ttcgcgacca tctttcgcgc gtggcccggg tgcacgcggt cgtccttcgt
     9721 gttcgtccag tagaacggtg tcgggtactt cgcgtccttc gtcaggagct cgtacggcga
     9781 ccacgtcttg atgtacgccc agtcctcggg cttgtcggga tctccgtact cggccatcca
     9841 gctcgctccc gcgagcagct tgttgtaccg gcgcatgtcg gcgagcggca cctgcgccac
     9901 gaccgcgcgg aagaggtccg ggtagagcgt gaacgtgccg ccgacgagga ggccgccctg
     9961 cgagccgccc atgatcccga ggtggcgcgc cgaggtgatc ttccgcgcga tcaggtcgct
    10021 cgccaccgcc gaaaagtcct cgaagttgtg gatgtgcttt tccttcacgg ccgccttgtg
    10081 ccactcgggc ccgaactcgc cgccgccgcg gatgttcgcg tccacgtaga cgccgcccct
    10141 cgcgagccac gcggctccga tcgtgccgct gtaccgcggc acctcggact gttcgaagcc
    10201 gccgtagccg tagagaagcg tcggggccgt cccgtccgcc ttgaacccct tcggcgtcac
    10261 gacgaagtac gggatcttcg tgccgtcctt cgacaccgcc tcgaactgct cggttttgac
    10321 tcccgtcgcg tcgaagaacg cgggcatcga cttgaccttg accggctctg atgtccccgt
    10381 cagccacagc gacgtcgggg acgagtagtc ctggtacgtg aagaagtacg cgttcgtgag
    10441 gtcgtccgcg gacgcgaacg acgccgtgcc gaggccgggc agcgccacct cggaccgctt
    10501 ccacgcgccg tcctcgaggg cgagcgccgt gatgcggctg cgcacgttgt cgagcgtctg
    10561 gatcagcacg cggtccttcg tgcgatccac gcgccccaac gagacgcgtg ggccgggctc
    10621 gaagaggacg tcgaaccggc ggacgccgcg gaggacgtcg tcgacggtgc cggcgatgag
    10681 cgaccccgcg cggtacgtct tgccgccggt cgtccagtcg ctgcgcaggg agatgagcat
    10741 ccggtcccgg aagaacccgc gcggccgcgc atcctcgggc atgtcgatct tcacgaggcg
    10801 gccgccgagg tagaggaacg cctcgatcct gaagaacgca ggggtgcgcg tgacgaggtc
    10861 gtaccggccg tcgctcagga tctccgaggt gccgtccacg gagacgtcct cggtccggcc
    10921 ctcgaacacg gtcttcgcct cggtaagcgg cgtgccgcgc ttccagagtt tcacgaaccg
    10981 cgggtaaccc gacgccgtgc gcgagccggg accgaagtcg gtcccgaccc agagcgtgtc
    11041 ctcgtcccgc cacgacacgc ggtgcttggc ctcgggcagc gtgaagccgc cggccacgaa
    11101 ctgcttcgac gccgtgtcga actcgcgcac cacggcggcg tccgagccgc cgggagagag
    11161 ccggaccaga cagcggacgt tcgccggcgg gaggcagacc gcgccgtgcc agacccaggc
    11221 cttgccgtcc gccttcgcga gtgcgtcgac gtcgaggacc gtctcccact cgggtgaggc
    11281 cgtgcggtac gaggcgagcg acgtgcgccg ccagacgccc cgctcgtgcg cgtcgtcctt
    11341 ccagaagttg tagaccgtct cgccgtggag ctcggggctc gggatcttct ccttcgagtc
    11401 gaggatctcc agcgtcttcg cgtagatcgg cttgtattcc ttgcgggccg tcagaagcgc
    11461 cgtcgacttc gcgttgtgct ccttgaccca ggcgagggcc ttctcgccct gcacgtcttc
    11521 gagccaccgg aaggggtcgt cgccggtctg ggcaacggcc gtcgtcgaga tcgcgagcgc
    11581 aagcgccccg agccacttcg tgaatgcctt catgtgtttc atccccatcg ccgtcatgag
    11641 atcggaggag tctatgacgg cgggcgtggc acgacgaacg ccattcggtc ggaatcactg
    11701 gcctctaaat tgtcagatca agggccgcga gcacgtgccg tgcgagagcg tccggggttg
    11761 tctccagagg gagtcttagg ggctggcccg tatggcgaga ggtgctttga gcagcctccc
    11821 gctgaaacga tagaccataa tcgcgcggct cgacgcctca agcgggacct cgtaaatgcc
    11881 ctcgccctca agtttcagaa ggtgggcgac gactctcgtc ttgggctcag tcatcagcga
    11941 gggtagctaa ttcacggacg acgcccaccg ggcgaacgcg cgttcagcga tttcacggcc
    12001 ctttcccttg atcattcccc gcaccaggtt ctcggttacc aggatcacgg acatttcgat
    12061 tccgtgctgg aacggcccca ccacgggctg ctgctgttcg gccgttgacg gatcaccgat
    12121 accccaatga aggctcggaa attgccgtcg aaaggtctcg ccgaggtagt aagcagcgcg
    12181 catcaccgcc gcagacgttt cgggcgttaa gtcaaaaagg ccctccgcgt atgactcagt
    12241 gttacgaatc cagggtggca gtgcagggtc gggctccttc gaaacgaccg ccgcgcgcgt
    12301 tgcgacccac tcgtagagcg gagcgagaga aggcagagtg aagttcagca acaacccatc
    12361 ggctgcggcc tctacggcga gtccttcgaa tgcctttccc tcaacctcca gaaagttctg
    12421 taggtacttc tccgcctctg cgcgggacag agactcgaag aactcaaact tcatgggtgc
    12481 gaggtatttg agatttcaag accccggaga gctttcatga ccgcggccgc ctccacgtcg
    12541 gtcattcccc tgaagcccga ccgcgcaatc atcacgaccg gactcgtgga cacggaatac
    12601 agacgcgcca tttcctcgta gcgggcccgg gactcgggtc ggagtttcgt taccacctct
    12661 gccattacct cgatcaatga ggacggggct actgcgaagt tcgagtcgtg gctcggccac
    12721 gtcacgtctc tcaggctgcc cccgacgact ccaaggtcat cgacgtaggc gctgctggcg
    12781 tcgagactcg acacagtgca atcgactagg ttgcaggacg agacggtgcc agagagcaca
    12841 agtccctgaa tatgcgtctg ccggaacatc aagtctctcg cgtccacccg gccgaattcg
    12901 cacgccacaa accgtgactc gatgatctgt gccccaccga acgccacctc tgaaaatgtc
    12961 gtgcgctcga acgtacagcc cttgatgagc cccatcaggc ggtgcccgaa ccttacacgt
    13021 tcaaatcggc atcccgtgaa cgacgttcgt tcgagccgcg ccccatccag cgccacgtcc
    13081 cgaaacgtgc aaccctcaaa tgaggcacgg ccccagagcc gtgggcggct gtgcacatcg
    13141 cctaggtcca actcgtggaa cgagcggttt gcgttaaccc gcctcgtcca gcgaaagccc
    13201 cctgggaatc tgacctgttg cagagccgtc gtgccattca ttgcgacgaa agcgccgcga
    13261 tccgtcagaa cattcatttc gtgaagagcg gatccaggct gagccacgca ggcctccccg
    13321 gggggcgggc ggatcactta cttccgcata gtaaagcccc ccggccgcgg cccggcgtaa
    13381 gctcttcccg tggacttcga gctgacgaag cgcgtcctcg gcgagttcga acggcaccac
    13441 gtcgactacg tggtcttcgg cgccgtcgcc gtcaacctcc agggcctcgc gcgcgcgaca
    13501 gaggaccttg acgtcttcat cgctccccgc gtggagaaca tcgagcgcct caaggcggcc
    13561 cttcgcgccg tgttcgacga ccccaacatc gatcagatca ccgccgacga tctcctcggt
    13621 gaataccccg ccgtccaata catcccgccg cagggcggct tccacatcga catcctcacg
    13681 aggctcggcg aggcgttccg gttcgaggat ctcgcgtcgg aaaagatcga cctcggcggg
    13741 gtatccgtca cggttgccac gcccacgacc ctgtaccgca tgaagaaggg caccgttcgt
    13801 cccaaggact ggggtgacgc cgaggccctc cgcgtgcgct tccggcttcc ggaggaatga
    13861 tgccggttcg gaggttccgg gacgtctccg aaatggaggg cacgctctgg cggaagccgg
    13921 gcgaccccga gcttttccgg gcgatcgctg gcgtctggga cttcgccgcg cgcaccgttc
    13981 cccgccattt cccgcctggg gtctaccgcc atcggtccat cgaggaggcg gaagccctga
    14041 gggagaagtg ggaagaggct gatttccagg ccctccacga ggatcgccgt cggaagggtt
    14101 aggcgagaga ccagagcccc gcgcccgggc tcaccgcatc agcccgtacc gctccttgag
    14161 cgcccgctct tccttctgca ggacgtcggc gcgcgagcgc agcgcctgcg cccgggtggt
    14221 gacgttcgcg agctgaccga tgggctcggg cgattccatc gccgtcttca gcgagcgcag
    14281 cacctccagc gcgtcgtcga ggcttccgag actctcgcgg tggtgggcga cgcacggcgc
    14341 gggcgggctg agcgcggcca ggcgcaccct cgccgcctcc gtctcccgga tcatcttctc
    14401 gaggctcgac gtgtcgccgt tcgcgagcgc cgccgccatc tcctggccca cgctctccgc
    14461 gttgccggtc atcgacccca cctggatccg ttcgaccgcg aagaagtacg ccgcgacggc
    14521 ggcccgcatc gagatgtccc cgccgccgcg ctcgccttcg gttggctccg gaacctggcc
    14581 ggcggccgcc tgcggtgcgg caggttgctc cggggcggac ggcgtcggcg cggagacggc
    14641 acgcgtgtcg ccatagtccg tggtcgcccc ggcaatccgc gcgggccgcg gaagcggctc
    14701 ttccttcgga ctcggggcga tgcgctcgat ccgcgccggc ggcgcgggga cttcaccaga
    14761 cccggaagcc ttccccagct ggtaggccag cgcgagaagg gccgcgccga gggcggccgc
    14821 gattccgacc aggaagccct tgctcacgct gacactcgcc atggttcgag gataccgact
    14881 caggggctct gttcggcacc gccccagcgc cccgcgccca tcggcgttcc ccgaaggcgg
    14941 acctgcacca ccgtgtcttc tccacgtgct agcgtccctt aagcgcatga accctcgaaa
    15001 gctcctccag aaggcgctgg cctcccccaa caacttcagg ttcgccgagg cccgtgccct
    15061 cgcggaagcg tttggcttcc gcctcaatcg tgtgagcggg agccaccaca tcttcgtgca
    15121 ctccgaggtt ccgcagctct tgaacctcca ggaggttcac gggcaggcca agccatacca
    15181 gatcaggcag ttgctccaac tcatcgagcg gaataatcta aagctcgcag gcgacgaatg
    15241 aaggactacc acatcaatat ctttcagagc gacgaggacg gcggctacat cgccgacatc
    15301 cccgatctgc cgcactgctc ggctttcggc gaaacccctg aaaaggccct cgaggaagtg
    15361 ctgaaggcga aggcagcctg gcttgacgcg gccagggagg cgggcaagcc catccctccc
    15421 ccgtccttca agccggcgat ctaccaggtc gcctaggttc ccgggcggct cgacgagccc
    15481 aaatttaagc tcccccctca gatccgcggc gtcgtcccgc cgatctcgag ccacgagaac
    15541 cacgccgggt ggttctcgcg gtaccactcc agcaccttgc ggatcgtctt ctgcttgtcg
    15601 cgcgggacga ccttgtcgtc gatccggtcg aagtgaatcc ggatcgtgtc gccgtcgtgc
    15661 tcgagcgcct gcgagaacat cgtctgcagc tcgttgcgga tgcggccgga gtcggagatc
    15721 ttcacggggc gcgtcgtgta gtccacgccc gacatgaagc ggacgaggag cgggctgaac
    15781 acgtggcgcg ccgtttcgag cggctccatc agccggaacg tgaacgtgat gaggctcgag
    15841 gcctggtgcg cggcgagctt cgtcgtcacc ttgtaaacgc cgtcgccgac tttcgtgacg
    15901 ccggccgtcc ccgtgtacgg gtcgtcgcag atgtagccgg tggacgccag cacccgccag
    15961 ttcttggcgg ggacgaagtc gccgggcttg agcgcgggcc ggcccgtcgg cacgttgcag
    16021 gccttcgcct cgcggatcgc ctccgcgtac gagacgaccc ggcccgcctc cacgtcccgg
    16081 ccgcccagca cctcgcgcac ccgctgcgcg aagccgggct tgtcggggtc gagcgccatg
    16141 agccttccct cggcgacgca cgggaagtcg aagccgggcg agaagagcga catcgaggcg
    16201 tccacgtcca gcacgtccgc gaggctggcc gcctgcatcg cctcggccgt tgcggcgtag
    16261 ccgtcgcaga ggaagacgtg cgtctccccc gccttgtccg cccagctccc gatgaggaac
    16321 gtcggcgcgt acgtccccga gtccgtgaac acctcgaggc ccgacggcgc cttccacccg
    16381 tcctccacga ggtgcacgcc cagcgccttc cactcctccc agagctctcc gaggcgcgcc
    16441 acgcggttct tgccgcgcag cgtccagacg tggatgttcc cgcgcttcac gccggggtag
    16501 gccgcctcga tcgcccgcgt cacgtcggcg cgcggcgtgc ggtagtcgag cagcagcgac
    16561 gcgcggtcgg ccgcctcggc cacgcgccgc ggcaccgtga ggcagcccat gtacgactcg
    16621 tacgggcgcg cgaggatgag cggcgagtcg aagaggtgga agaccgacat cgggcccgtc
    16681 gccttcccct cggcgaagcg caccgtgttc tcgagcgtgt ccaccgcggc gccccagatc
    16741 gtgatgcccc gtgacttgag ccagtcgtgg aagtgctcga acgtgtgccc ggactcgttc
    16801 agcagccggc gcacccgccg gtccacgacg cgcgcgacct cgggccgggc gtaaacgcgg
    16861 ccgaagccga ggagcgggtt cgcgcccatc tccgccgtct cgcccgcctt cggcatcaac
    16921 ccctcgccga ggcagaccat gatcgcgtgg ttctcgggca gcgtgcgcgt caggtgccag
    16981 agcccctcgc tcatcacgta cgcgctgatc gcgtccgccg ccttcttcgc ctggttcaga
    17041 gtcgccttgt cggccccggc cccctcgccg tagccgccga agaggcgcgt cccgagcgcc
    17101 gtggccgccg acgtcatcgc gaggcaccgg gcgatgccgc ggggcgtgaa cgagtggtcc
    17161 gagacgggct cgggctgcga gcgctcgagc tccacgtcct ccagccacag gtggaaacgc
    17221 agaagcaccg gacgggtgtc gaacgcccac tgggcgacca gggtgcgctt gtcgatgggt
    17281 ctcatcctcc cctcgagccg acgtcggtgt tgtccgtaca gtatcgagcg gcgaggggag
    17341 cggcaagcct cagaaggggt tcgtctggcc gtcgtcccta tttgcagtgg gtgagccgct
    17401 actctcgatc gacccagtag cctggccggc gccgctggtg ggcaacagca aggatccgaa
    17461 caaagtcggc ctccggcgag tagtacagct cgtacggaaa ccgccgcagg agcttgcatc
    17521 gcacgcttcc ccgctccacg ggcgctccct cggggtactt ctcgatgaag tccacggctt
    17581 cctgcacatc tcccgcaaat gcgtcgacgg cgctcggggc gtggagcccc agataggcga
    17641 ttacttcgag gtactccgcc tgcgcctcgg ggtgaaagcg agccttagcg ggacgtgacg
    17701 gcccggccac gggcgaatac ctcgtctgcc ggtatctcgc gtacttttcc ttctcgcatt
    17761 tccgcaaggc gccgctccgc cacctcgagc cacagcgtct tccgttcggg ctccggcagc
    17821 tcgtcgaggc tcctcaggag acggtccgca agcgcggccc gctcgtccaa cggcagcttc
    17881 aaggcggcgg cgagaaggtc ttcgcgcgtc acgaagggaa ttgtatcccc ggcagcactc
    17941 cggtcgccct cacgcccgaa ccttcttcag cccgtgcagg acggcccagg cgtcctcgta
    18001 aaccttctcg cgcaccgggt cggccttgta gatctcgctc ttgaagttcg ggtagtcgag
    18061 cgcggccacg cgctcggcga cgaaggccgc aagtacgtcg cgcgacgtcg tgagccggaa
    18121 gccgtagtcg gcctcgggcg actccaccac cgccggccgc ccgccccgct gcgccgcgaa
    18181 gtcggcgaac ctttcgagat ctgcccgcac ccgcccgcgg acgagcagcg cgtcgtcagg
    18241 gctcgtggaa tccaggttcc ggacgacgga gagaaagccg gatgtcgtga tgagccacat
    18301 ctaccgggaa ggtagcgccg gtggacggac atcgggtgtc cgtatgatcc caggggctag
    18361 aacgttgcgt agaggccgta ccccacagcc ttcttcagaa tcgcctccgc tcgttccacc
    18421 gcagttgtgc gtcgcgcacg cctgaggtcc gagagattcc gcacgacatc ctcgggcgac
    18481 tggaagtagg aacccgttcg gccaggatca aagggattct ccgggggacc gaatggagtg
    18541 ccgagaagcg tgaggccgag cccttcaccc aacgacacca gcgcccggtc gacgtccatc
    18601 cagtccgacc caagcccgag gttcagcacc ggcgagtaga acttcgtcag cgcgaagtca
    18661 ccgacctcgt gctcgtttcc gaactcgagt cgggcctccc agtctcgcgt cagcggttca
    18721 ccctcgtagg ggtcgcgaaa cgactcgagg ttcgagttga tgagtctcct cagctcgtcg
    18781 cttgatcgta tttcgagggc ccgaagaaga gcaggggcta attcgacgtt gaatcgccgg
    18841 tagtcgaaga cgaatgcctt gtgatggagt gacactgaaa gacgtccgac ccgttcgcca
    18901 acactatcga aagaaatcct cgaggaaagc ctcgaacgag ttccacgatg gcggaagtct
    18961 gccctccaca atcaggcgct ccccaccttc gaagtccacg ttgtagaccg cacccgtggc
    19021 cccgtccagc accagaacgg aattcccgag cagatcagtg agtacgacga gtcgctcggg
    19081 ccacccaaag cggccgcggc actcgatggt cgagctcacg atcgtcgggt gaccctcgca
    19141 gatgtcgagc agttcgaatc cgctgctctt gctaccgaag ggcccgacat aagacctgaa
    19201 gaactcggtg aacgcctcgg gaagaggcac gccaagacgg ctggaggctt ccgctgctgg
    19261 aaagtcgtcc gctctttgaa atgctggggc gacgagcttc ccgtgaattc gctctctcca
    19321 ctcggtcatg ctctcaccgg tgcgcttcac gccattgcca gtattcaggg ctctcaatcg
    19381 tttgaaacgc gtcgcgtggt gaacccggac cgcggccaag gggtgcgtat gggttctgac
    19441 ccccttcgta cgggtgaagg ggaggctgtt gggcggtcct gatgttgtgg taccgagttg
    19501 acgcctcgaa ccagggcccg gtcgcacttt gttccgcatg atgcagggtc gcgaactcgc
    19561 cgtcgggtag ctgcggccgg aagccggcct tcgctgcttc ggcattcgtt ttcccaatga
    19621 agtctcgcgg tccagctgtt ctcacctggc tccagtctat gtctgcgcgc tggtacaccg
    19681 tgtaggtacg acctgtgctg ggagtaaatc ggacagagct cgggaaggat accgatccag
    19741 tagcagaccc ggcgcgcccg ctagcctggg ctggccccac tccctcaggg aacatcacag
    19801 gcgtctcggg gtatgaaggc cagagttccc gcgcgagaag cctgtagccg ctttcggatc
    19861 cttcttgggc cgcacgcttg agcacggttg aagctattcc acggccgacg aggccaagca
    19921 ccttcccggc accgtaggcc gtcgcaattt cggtttctgc gtggatcgtg aatcgttgtg
    19981 cgcgagccag ttcctgagac gtcatcagtc cgagagcctg agcacgtgat gcgtcggtcc
    20041 cgaggttgat tccgttcgac tccatgaact cgtcgagcgc ggagcctgct aaacccccgc
    20101 ggcggagtag ttcaacggtc gaggctgggt tcgcgtaggc caatgcccat ggaatcaagt
    20161 accagtagcc gttgttccgt tcgtccgaga taacgatccc gcgcttcgag gtgctcaccg
    20221 ccagccccaa cggatccacc acctcatgcg gcctgcccgc cccgaacagg tactggttcg
    20281 gcgagtcgat gtcctccagc ggatccggcg aaaggaacgt ccccgttcgc gggtcgtacc
    20341 agcggttgcg catgtagatg aggccggtga cggggtccgt ccagtgcccg tgccagccca
    20401 gcgcctgccc gcccgggaac gccggccctg ccgacgctgc ctgaacgttc gcctgcgcca
    20461 ccggccgccg cggctcgtga cgcgtctcga acggcccgga cttcggcacc gtgaagctga
    20521 agtcctcggc gttcgtcagc ggctgcccgc gcacgtcgcg gatggcgtcc gtcacgcgca
    20581 cgcggtacgt cgcgccgcgc tcccatccgc ccaggggtcg caatccgatc tcgttgacag
    20641 agccgccgtg cgcgctcggc gcgatcgtcg cgctgccc
//
LOCUS       DAOMFY010000040        20608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_826_length_20608_cov_7.623559, whole genome shotgun sequence.
ACCESSION   DAOMFY010000040 DAOMFY010000000
VERSION     DAOMFY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20608
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_826_length_20608_cov_7.623559"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLB01_05430"
     CDS             complement(<1..1007)
                     /locus_tag="PLB01_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQQ76775.1"
                     /translation="MSDSSVTTTSGLPVKASYGPADVPAGWDPGKPGSAPFTRGTLPG
                     GYRTKLWTMRQYAGFSSAKESNRRYRFLLDRGQSGLSVAFDLPTQIGYDSDHALARGE
                     VGKVGVPIASLEDMETLLDGIPLDAVSISMTINATASILLSLLLAVAKGRGTPWAKLS
                     GTIQNDILKEYAARGTYAFPPKPSMRLVTDVFAFCQKEVPRWNTISISGYHIREAGST
                     AVQEVAFTLADGLEYLAAAREAGLDPAAIAPRLSFFFNVHNDFLEEIAKFRAARTLWA
                     RLLKDRFGIEDPRSQALRFHTQSAGSTLTAQLPDVSVVRVGDQALAAVLGGTQSLHTN
                     SR"
     gene            1045..2115
                     /locus_tag="PLB01_05435"
     CDS             1045..2115
                     /locus_tag="PLB01_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQQ76776.1"
                     /translation="MALRRLPALLLAALLAAIPSHAAEDPLLLSFQEAKKAYREKRWD
                     DADAALRRLLELAAAPGREPVLAKILPAYHFYAAAVAWERKDEERARQELARYFEFQP
                     EATIDPGAYPKSYCIFFDAQRTAAERRNPAPPPAPGLPDFGTTLPDEGTVPQYSGDPA
                     WPATAVSFLLTESEKRDFAALGDDAARREWVFRFWKKFDPDPATPDNEAEREFYRRVQ
                     YAEAHFSTETMRGSLSDRGRVLVVLGPPSYVGRSPLLQSEDTMTQLKTSQPTIVRSPT
                     GGASLLRVPTTSRGGVTPGDIEGDVEIWYYRQDRVPKGLPFQELQYRFISKKGYGEAV
                     FQKDARELIALQRAARLLRTGS"
     gene            2163..3599
                     /locus_tag="PLB01_05440"
     CDS             2163..3599
                     /locus_tag="PLB01_05440"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HQQ76777.1"
                     /translation="MARTLSGNLDTFSLADLLQWLEINALSGRVTVWRGEVVRTIDLK
                     GGAIVFVSSSLPNERLGVFLTRRKVLSEPVVFELLAESFATGRNLTRLILERNLLPRE
                     KLAEAVEGLAMKVLLDLFHWAGASFEFDPLFKIEDLIRIHLSLRGQVLAFHGAKSVDD
                     SRVNVGAAAGGDETGARWEREFKAESLAAMFWEILEGLPGETPTPAQLKESYQGFTSF
                     ANRVHKRLREPSRLFPVYDDTAVMLKSALDEGGDPERIVQVAALDPFLTIDLLYLANA
                     LRTDASELVRTSREAAGVLGSAALGRFVALLADSSTPKVPSEEKMERVIRRAALSTAV
                     AASHLAEGTEIPREEAYTLGLLEPLGSYELLKLLISVPFPPGPIRIGALSRFRAAAGR
                     VLARKLNLPQPVEDVLGSSGRVASRSPAAEQLIFLAKQIAPSEQIGHEWTTEDPKLSD
                     QVVALSARAELPQLIARDASMLREILQL"
     gene            3626..5302
                     /locus_tag="PLB01_05445"
     CDS             3626..5302
                     /locus_tag="PLB01_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ76778.1"
                     /translation="MTQAAPARRSGALTAGAFVAALTVAVAAAAQQPEPPRILREGVR
                     AAVDVTVMDLDVVATGKGGEPVKDLAKGEITVIVDGIPYTPDYFARIDAGQVHGPDLA
                     NASPDVVLETTRAGDTRWVPRQFLAFFDDEHLMPFERKRVIEGLRDFVTRLSPSDSMA
                     LLAYNISTRVFVPFTSSKEDLLLGLSKLEKLPPRGLYWDTQYRQAIRDARRYSPVSVQ
                     GQAARDSIVRTWGAQAWTRDRSTLEEFRRSVDALAARSGKRVLLYVSHGMEMRPGQSL
                     AQSLGGTALNQFDYDVTPQYRAVLESANRAGITLHAIDALGLVAGGDASESDPPAIDP
                     FLANANRREILAGFADETGGVLVENRNVFAASLDRIYRESASYYSVGVTLSSLNAKKP
                     THEVRLRTTRAGVTLRTRRTFAPLTAPQAARDRLEMALITPDAAGDFAVTVRTDAPKK
                     GGGMGRRLVPFAVVVPVSSLTFLDEGGKKKAVVEIGLAAVEDTGARSAPVTQRQEIVV
                     TPEVLAASGKEPFVYRGEFKSGTGNMRFVATVRDVTSNRVGVGSASVRVE"
     gene            complement(5306..6865)
                     /locus_tag="PLB01_05450"
     CDS             complement(5306..6865)
                     /locus_tag="PLB01_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQQ76779.1"
                     /translation="MKRSTSALVLFLLLVSAAGPAAAALDKYKDWEKSPEYQYLATDA
                     DKKDWKKAASDEDAEKFIALFWARRDPDIKTPRNEFRERFDALVKLADERFTLGKKRG
                     ALTERGRALILIGPPKEITAKATSSADTPFGTGSAGGVTGTGGGAAMLYTFVYDKSHL
                     PAWSDVQSQEIKFIVDVGLGTESLLDMSPARRLETKAAQVAVVSPDLKEVPVYKTREQ
                     VEAEQKKLAEDAKGPALSPASRAAIDGLPKEPFGPLALLSLAYRDGATRLSVQVYAPA
                     AAVGTGEGVRLAIVARAADGSEAARVDEDAKLRKTRSDFFADRALRVLPGDYDVAAVL
                     LDAAGKVLVSGRRPITVPALPTEFSASALVVAVNDFPTDTPAADDPFTFSARRFVVKG
                     DGKLDPADGLSYAVRIYNPPVDPVSRTLTLRRTIKIKPKGQPPIDVPTPPEEPQKVPP
                     SQEGGALVLDLAGGVIESNLGQYLKPNDYELRVTILDTVTQKKLDLVAPFSVVGSAAP
                     APAPAAAPKKK"
     gene            7244..10249
                     /locus_tag="PLB01_05455"
     CDS             7244..10249
                     /locus_tag="PLB01_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ76780.1"
                     /translation="MTRKRILGLLALLLVVVVGATSAMGQSTTTGNLTGKVENENAGL
                     PGVTVTIKSPNLQGTRTATTSAAGAFTFAALPPGEYTVSFALQGFQSITKTVKVTAGQ
                     QSALDVNMTLSGVQASAVVTAKSDIVSTTTQASTTITNDLSNTLPNTRTIAQAAALSS
                     GVTPVVSLGNALTIGGAPTFDNLYTVDGAVITDNIRATPNNLFVEDAIQETTTNVNGI
                     SAEYGRFQGGVINTVTKSGGNTFSGSFRTTFVNDAWSAVTPQGETRVQDLQERYEATL
                     GGPIWKDHIWFFGSGRSAKVGTAQTTFADAKVGGATPVGGYAGIPYTNTLDEQRYSGK
                     LTLTPIQNHTLTASYLSISNKEGNNVFGVVLDEKSFVQRELPQDTLSIAYNGVFTSNL
                     FFEGMYSNRHFTFKNSGSTFTDVINGTLMRDRSRGNARWNSPTFCGVCGPEKRDNEDV
                     LLKATYFLSTGSLGSHNIVLGYDTFKGIRTSNNYQSGSNYRIFHTSTINYNGTIYPVI
                     NNVGTSSFIYYTPIFQLTQGTSTKTDSFFLNDQWRLNDRLSFNLGVRYDKNNAKNSVG
                     ATTANDSAWSPRLAVNWDVTGKGNVRLGASYAHYVGGLQDSIQDSTSAGGQPATFYWY
                     YNGAGTAPINVGVPAGTQPPVSAAQAIQQVFNWFYAQGCPDLATCKLPLAYGSIPGLN
                     RAIDGTVKSPYAREYTIGIAGNIGSSFSYRADVVRREFHDFYNLVVNTQTGYVTTPTG
                     AKLDFGLIGNTNSVERNYTGLQTQFQYRWQWLTIGGNWTWSHTLGNFDAEAGNSGAVT
                     NATEAYPEYKQMSWNAPYGSMAADQRHRVRLLASADLPFIPKSLGNFNLGLIQGYDTG
                     SPYGAVGTVRASNYVTNPGYQQRPSTSTYYYTARDEFRTDDIKRTDLSLTWTLRLFNT
                     LELFVQPQVLNVTNNQGAIAVNQTVQTLNNTTGYLHFNPFTTTPVRGERNVANPTANW
                     NYGPSFGQVTSVAGWQQPRTFLLTAGLRF"
     gene            10328..12502
                     /locus_tag="PLB01_05460"
     CDS             10328..12502
                     /locus_tag="PLB01_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQQ76781.1"
                     /translation="MKSGRPAAAVLACAIALVSGAAGCRRGPAAAAFPGAPVILVSVD
                     TLRSDHLPAYGYKGVETPALDALRKDSILFTRAWAHVPLTLPSHASMFTGREPSAHGI
                     HDNLGYRLRADVPTLAELLKKEGYETGAAVSCFVLKGAASGISRGFDFYDDAVEPAEG
                     AAALGRVQRAGPETVARLETWLAARPTSGKTFAFLHLYEPHTPYEPPEPFRTRYAAQP
                     YDGEIAESDAIVGGLVAFLKARGLYDRAVIVFLSDHGEGLGEHGESEHGMFLYRESLQ
                     VPLFVKLPDSRRAGETVDEPAALFDVFTTIAEGAGARGFSRPEGTRSLLDPPGAPARR
                     ILSETFFPRIHFGWSELSSLFDGRWHYIEAPKPEIYDLPADPGELKNLYAQKPDALRP
                     MLAEIAKRKPVYESAGDLDPEARKKLASLGYLSAGPSSANAGGLDDPKDRIRTFEELR
                     VGLGDLTAGNSAKAHAIFTKLLAENPRMLDVWDMDSKVLIQLGRPEEALAALKKTVDL
                     APEAARTPYLREVANLCLQLGKWDEALNHAEALRALGDPDAEDIAARGFLGKGDLAAA
                     GPAARAAYEKGSGKTKVRGALVLGRLAVMRGDVASADKWSQEAAALSADDKLVQSGLH
                     MLRGDVLARQGRAPEAEKEFLEEIRLYPGRMDARVSLAALYASAGRREDARRTLIALV
                     VHEPKPEAFLLAMQAFRATEDPDGEREMRREARRRFPRDPRF"
     gene            complement(12507..14069)
                     /locus_tag="PLB01_05465"
     CDS             complement(12507..14069)
                     /locus_tag="PLB01_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQQ76782.1"
                     /translation="MKTSIRVLALLLLAASVPGLGAGLERYKDWTKSPEFVFLSTDAE
                     QKEWKKITADEQADRFIQLFWARRDPDLKTPVNEYKLGFDQRVKEADQLFAMPRQRGA
                     LTERGRAYILLGPPKTLQRSAGTKAQPVAAPGSANIPPPNEPGTSIGESKVTFIYEAA
                     QLPEWAKMKSLTAEFVVEQASDFVAGRSSGDVKRLEVNARAALLKNPDLKEAPHYRTA
                     AELEAERQAALAAEAEAKKGPPLTPAIRTSLEGLAEDSALLAVVPLAGGETDDTRIQA
                     QLFLTAAAGEPPAGSHLAFLVRTPDGKDAARYEESTPLEPAPGGGLYASRWFSVPPGE
                     YTVAAGIFDGSGKAVAAARKSVSVAPVPTDFAVSPLVIASAFFIVPKPKPEDAFTFSG
                     HRFISKGARLDPTDGLSFVLRVYNPAVDPATKTISLSRTVRFRPKGAQWSDVPQPQDP
                     PTPAPDRKDQASGGILTVDVSAVLIETRLGEYLKKPGDYEIKVAVTDNVSKKKAEAAT
                     TFVVTGTLPPKK"
     gene            14297..17335
                     /locus_tag="PLB01_05470"
     CDS             14297..17335
                     /locus_tag="PLB01_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ76783.1"
                     /translation="MSISTTSTNARRLSILVGVALLVGLALAPVVLAQGLPTATLSGR
                     VRNDALDLPGVTVTAKSPALQGTRTTVTRANGDYVLANLPPGDYQITFAIQGFKTQTL
                     SKTLGASQQATLDANLAMTVTAATTVTAQSDSISQSPAQATTYTGDVLSKLPTARTVT
                     AAVNLSPGVNQNGPNGASISGAQSTENLYTVNGVTITDNVRSSPNNLFIEDAIQETTT
                     TTSSVSAEYGRFTGGVINTITKSGGNVFSGSFRSTLNNNAWNSTSGYRTATGVNPQEG
                     TFTNTVTSTYEATLGGPILKDMLWFFGAGRYFDTSEALTSVTRFTNIPYTNGAKELRY
                     EGKLTFSPVQNHTLTGSYIGVKHDDVNYYFTSIPIADLASIYDRQLPQELMAFNYNGV
                     LSSNFFLEGSYSKRKFTFENSGGRFQDVANGTVIRDLGLGISYNAPIFCGVCGPEKRD
                     NNEFFVKGTYFLSTPGFGSHNLVAGYDNFAGQRLSNNYQSGSNYVIYTFGSGRSVFQG
                     QNVYPVFEPGLTELDYWPVLQASQGSDLRTQSAFLNDAWRLNDRLSFNIGVRYDKNDA
                     TDAAGNVTSKDSKFSPRLAATYDVTGNGSLRLTASYAQYVAALQETQAGSGATLAGSP
                     ADFYWYYDGPAINTGAGPYLTSQQATQKIFDWFAANGCLPNPLAASCKVPQGGAPSIG
                     GVNVQIRDSLASPNTNEYVFGIAGNFGKSTQGSYRVDFVRREYKDYYDLKKDTTTGKV
                     ASPFGDIQDLGLVVNSSDYRREYTGLHSQVFYRFTPSFTLGANWTWAHLIGDIVGETS
                     GSGPVRGGSHVYPEYVQRSWNNPVGDLSQDQRHRVRVYGNYDLPVPPSWGSFNIGFIQ
                     TYDTGTPYGAAGTIRTGSYVTNPGYLTPPASVTYYFTPRDAYRTETIWRTDMQLYYSH
                     RIVGGLEIFVIPQVFNVFNAQHITAVNTTVNTNFNTSNLLPFNPFTNASPVECPQGTS
                     GAACKAMGANWQKGSLYGTPTASSSYQTPRYFQLSLGLRF"
     gene            17412..19595
                     /locus_tag="PLB01_05475"
     CDS             17412..19595
                     /locus_tag="PLB01_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQQ76784.1"
                     /translation="MFSTARTRTRLIAALGTVVLAAGCGRPRSSARVFPKAPVVLISI
                     DTLRSDRLPFYGYAGVKTPALSALRDDSILFESAWSHAPLTLPSHATVFTGLLPAQNG
                     LHDNLGYSLNPSVPTIAELLGKAGYATGGAVTSIVLSGTTGIGRGFGLWDDDVVPTRA
                     FQALSRVQRSGDEAEASMERWIEKQSGPFLAFLHLYEPHAPYEPKEPFRSLYASPYDG
                     EIATVDAITGKFLDFLKAKGLYDRSLIVLMSDHGEGLGDHGEAEHGVFLYREVLQVPL
                     LVKLPKGDAPAPRSVAAPVQLSDLFTTIGKAVGLEGFAPPAGTVSVIDLADTAPQPAR
                     RIYAESFFPRTHFGWSELRSLVDGRWHYIEAPRPELFDLVADPAEKSNLADGRPEPFR
                     KLRIEMEALRTSFAAPAAADHETAKKLASLGYLSSGQAAGSGPLDDPKDHIATVGLMK
                     EALREFVEGSAAKSVAITQKLLAENPRMLDIWELQSQALAKQGKTDEALASLRKTIEL
                     APPGSTQYVVSFANHALQMGKAEEARKHAELARSMGDPAGDEILARACLALGDLPAAE
                     AAARECLKSDRNRDKGLLVLAQVEARRGNLPKALEASNRVQEGVVHALPLAGLHLLRG
                     DVLARMGRPKEAEAEFREELRAYPTTLKAWSSLVILYATQNRTADARKAIEEMVAAAP
                     GVDAYLAAYQTLSVLGDRAGAERWKQEGLKKYPGDARFNRPARPA"
     gene            complement(19605..20063)
                     /locus_tag="PLB01_05480"
     CDS             complement(19605..20063)
                     /locus_tag="PLB01_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQQ76785.1"
                     /translation="MSDSVTRGVFVRVAPERAFEAFVRVSEVLSWLADGAVIGARAGG
                     NWALGWYADPDSDAGYSSMGQIETFEPGRTLAVSNLVFSSPEGLSFGPMRLVVTFEAV
                     EEGTDVTVVQEGIEEGPVWESYRDQLGPGWERMLGGLKAWLEEGKKLPGR"
     gene            20101..>20608
                     /gene="argS"
                     /locus_tag="PLB01_05485"
     CDS             20101..>20608
                     /gene="argS"
                     /locus_tag="PLB01_05485"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQQ76786.1"
                     /translation="MLIGLKSALERAIVDACVKTFGVEPPRLVLETPPKVELGDLACP
                     VAFELAKVLKRPPRKIAEELAPALVLPDVVVKRTVEGGGYLNFFVDRPKVLRAMLEDT
                     HDAQSAGAADAGKLIVEHTNINPNKAAHIGHLRNAVLGDVLVRNFRRLGRNVEVQNYL
                     DDTGVQVAD"
BASE COUNT         3284 a         7508 c         6742 g         3074 t
ORIGIN      
        1 cgcgagttcg tgtggagcga ctgcgtgccg ccgagcaccg cggcgagcgc ctggtcgccg
       61 acgcgcacga cgctcacgtc gggcagctgg gcggtgagcg tcgagccggc cgactgcgtg
      121 tggaagcgga gcgcctgcga acgcgggtcc tcgatgccga agcggtcctt caggaggcgg
      181 gcccagaggg tgcgagccgc gcggaacttc gcgatctcct cgaggaagtc gttgtggacg
      241 ttgaagaaga acgacaggcg cggcgcgatg gcggcggggt cgagcccggc ctcccgggcc
      301 gccgccaggt actcgaggcc gtccgcgagc gtgaacgcga cctcctgcac cgccgtcgag
      361 cccgcctcgc ggatgtggta gccggagatc gagatcgtgt tccagcgcgg aacttccttc
      421 tggcagaacg cgaagacgtc cgtcacgagc cgcatcgagg gcttcggcgg aaacgcgtac
      481 gtgccgcggg ccgcgtactc cttgaggatg tcgttctgga tcgtcccgga gagcttcgcc
      541 cagggcgtgc cgcgtccctt tgcgacggcg agaaggagcg acagcaggat cgacgcggtc
      601 gcgttgatcg tcatcgagat cgacacggcg tcgagcggga tcccgtcgag cagcgtctcc
      661 atgtcctcga gcgatgcgat cgggacgccg accttgccga cctcgccgcg cgcgagcgcg
      721 tggtccgagt cgtagccgat ctgcgtcggg aggtcgaacg cgacggagag gccgctctgg
      781 ccgcggtcga gcaggaagcg gtaccggcgg ttggactcct tcgcgctcga gaagcccgca
      841 tactggcgca tcgtccagag cttcgtgcgg tagccgccgg gcagcgtgcc gcgcgtgaac
      901 ggcgccgagc cgggcttgcc gggatcccag ccggccggca cgtccgcggg gccgtaggac
      961 gccttgaccg ggaggccgga ggtggtcgtg acgctggaat cgctcatgag gccgatgcta
     1021 agctggtccg gcagaggttc ccaagtggcc ctccgtcgtc tcccggctct cctcctcgcg
     1081 gccctcctgg ccgcgatccc ctcgcacgcc gctgaggatc ccctcctcct ctcgttccag
     1141 gaggcgaaaa aggcgtaccg ggagaagcgc tgggacgacg cggacgccgc gctcaggcgc
     1201 cttctcgagc tcgccgccgc gcccggccgc gagccggtcc tggcgaagat cctccccgcc
     1261 tatcacttct atgcagccgc ggtcgcctgg gagcggaagg acgaggagcg ggcgcgtcag
     1321 gagctcgcgc gctatttcga gttccagccc gaggcgacga tcgaccccgg cgcgtacccg
     1381 aaaagctact gcatcttctt cgacgcccag cgcacggccg ccgagagacg caacccggcg
     1441 ccgcctccgg cgcccgggct tcccgacttc ggcacgacgc ttccggacga gggcacggtc
     1501 ccccagtact cgggcgaccc ggcgtggccc gccaccgcgg tctcctttct cttgacggaa
     1561 tccgagaagc gggacttcgc ggcgctcggc gacgacgcgg cgcggcggga gtgggtcttc
     1621 cggttctgga agaagttcga cccggacccc gcgacgcccg acaacgaagc cgagcgcgag
     1681 ttctaccggc gcgtccagta cgccgaggcg cacttctcga cggagacgat gcgcggctcg
     1741 ctctcggacc gcgggcgggt cctggtcgtc ctgggacctc cgtcgtacgt aggccgctcg
     1801 ccgctcctcc agtccgagga cacgatgacg cagctcaaga cgtcgcagcc cacgatcgtc
     1861 cgcagtccga caggaggggc gtcgctcctc cgcgtcccga cgacctcgcg cggcggcgtg
     1921 actccgggcg acatcgaggg cgacgtcgag atctggtact accgccagga ccgcgtcccg
     1981 aaggggcttc ccttccagga gctccagtac cgcttcatct cgaagaaggg ctacggagag
     2041 gccgtcttcc agaaggacgc ccgcgagctc atcgccctcc agagggccgc ccgcctcctc
     2101 cgcacgggct cctgacccgt ccgggcgccg gcgcgggcgt tcgtgtcaag attggtccac
     2161 acgtggcacg cacgctcagc ggcaacctcg acaccttctc gctcgccgac ctcctgcagt
     2221 ggctcgagat caacgccctg tcgggacgcg tcacggtttg gcgcggcgag gtcgtacgca
     2281 cgatcgacct caagggcggc gcgattgtct tcgtctcctc gtcgctcccg aacgagcgcc
     2341 tcggcgtctt cctgacgcgc cgaaaggtcc tctccgagcc cgtcgtcttc gagctgctcg
     2401 ccgagagctt cgcgacgggc cggaacctca cgcgtctcat cctcgagcgc aacctcctcc
     2461 cgcgcgagaa gctcgccgag gccgtcgagg gcctcgcgat gaaggtcctg ctcgacctct
     2521 tccactgggc gggcgcatcg ttcgagttcg acccgctctt caagatcgag gacctcatcc
     2581 gcatccacct gtctctccgc ggccaggtcc tcgcgttcca cggcgccaag tcggtcgacg
     2641 actcgcgcgt gaacgtcggg gcggcggccg ggggcgacga gacgggcgcg cgctgggagc
     2701 gcgagttcaa ggccgagagc ctcgccgcga tgttctggga gatcctcgaa ggcctccccg
     2761 gcgagacgcc cacgccggcc cagctgaagg agtcgtacca gggcttcacg tccttcgcga
     2821 accgcgtcca caaacgcctg cgcgagccgt cgcgcctctt ccccgtctac gacgacaccg
     2881 ccgtcatgct gaagagcgcg ctcgacgagg ggggcgatcc cgagcgcatc gtgcaggtcg
     2941 cggcgctcga cccgttcctc acgatcgacc tcctctacct cgcgaacgcg ctccgcaccg
     3001 acgcctccga gctcgtgcgg acatcccggg aagccgcggg cgtcctcggc agcgccgcgc
     3061 tcgggcggtt cgtggccctc cttgcggact cgtccacgcc gaaggtcccg tccgaggaga
     3121 agatggagcg cgtcatccgc cgggcggcgc tgtccaccgc ggtggccgcc tcccatctcg
     3181 ccgaggggac ggagattccg cgcgaggagg cttacacgct cggcctcctc gagccgctcg
     3241 gcagctacga gctcctcaag ttgttgattt ccgtgccttt cccgcccggc ccgatccgca
     3301 tcggggctct ctcgcgcttt cgcgccgcgg ccggacgcgt gctggcccgg aagttgaatc
     3361 tcccgcagcc ggtcgaggac gtcctcggct cgagcgggcg ggtcgcctcg cgcagcccgg
     3421 ccgccgaaca gctgatcttt ctcgccaagc agatcgcgcc gtcggagcag atcgggcacg
     3481 agtggacgac cgaggacccc aagctctcgg accaggtcgt cgcgctgtcc gcgcgcgccg
     3541 aacttcccca gctcatcgcc cgcgacgcgt cgatgctgcg cgagatcctc cagctctgac
     3601 gcccttttcc cctcgaggtg aaccaatgac gcaagccgca cccgcaaggc gctcgggcgc
     3661 cctcactgcc ggcgccttcg tcgccgcgct gaccgtcgcc gtcgctgccg ccgcccagca
     3721 gcccgagcct ccgcgcattc tccgcgaggg cgttcgggcg gccgtggacg tcacggtgat
     3781 ggacctcgac gtcgtcgcga ccggcaaggg cggcgagccc gtgaaggacc tcgcgaaagg
     3841 cgagatcacg gtcatcgtgg acggcatccc ctacacgccg gactacttcg cccgcatcga
     3901 cgccgggcag gtccacggcc cggatctcgc gaacgcgtcg cccgacgtgg ttctcgagac
     3961 gacgcgcgcc ggcgacacgc ggtgggtccc ccgccagttc ctcgccttct tcgacgacga
     4021 gcacctgatg ccgttcgagc ggaagcgcgt catcgagggg ctgcgcgact tcgtcacgcg
     4081 cctctcgccg tccgactcga tggcgctcct cgcgtacaac atctcgaccc gcgtcttcgt
     4141 gcctttcacg agctcgaagg aggacctcct cctcggtctc tcgaagctcg agaagctccc
     4201 cccgcgcggc ctctactggg acacgcagta tcgccaggcg atccgggacg cgcggcgcta
     4261 ctcgccggtt tcggtccagg gacaggcggc gcgcgactcg atcgtgcgga catggggagc
     4321 gcaggcgtgg acgcgcgaca ggagcacgct cgaagagttt cgccggagcg tcgacgccct
     4381 cgccgcgcgc tcggggaagc gcgtcctgct ctacgtgtcg cacggcatgg agatgcgccc
     4441 gggccagagc ctcgcgcagt cgctcggcgg gacggccctg aaccagttcg actacgacgt
     4501 cacgccgcag taccgggctg tcctcgagtc cgcgaaccgc gcggggatca cgctccacgc
     4561 gatcgacgcg ctcgggctcg tggcgggcgg cgacgcctcc gagtccgatc cgcccgcgat
     4621 cgacccgttc ctcgcgaacg cgaaccggcg cgagatcctc gcgggcttcg cggacgagac
     4681 gggcggggtc ctcgtcgaga accggaacgt cttcgcggcc tcgctcgacc gcatctaccg
     4741 cgaatccgcc agctactact ccgtgggcgt gaccctctcg tccctgaacg cgaagaagcc
     4801 cacgcacgag gtgcggctcc ggacgacccg cgccggcgtg acgctccgga cgcgccgtac
     4861 cttcgcgccg ctcacggccc cccaggccgc gcgcgacagg ctcgagatgg cgctcatcac
     4921 gcccgacgcg gccggcgact tcgccgtgac ggtccggacg gacgcgccga agaagggcgg
     4981 cggcatgggg cgccgcctcg tcccgttcgc ggtcgtcgtc cccgtctcgt ctctcacgtt
     5041 cctcgacgag ggcgggaaga agaaggccgt cgtcgagatc gggctcgcgg ccgtcgaaga
     5101 caccggcgcg cgctcggcgc ccgtcaccca gaggcaggag atcgtcgtga cgcccgaggt
     5161 cctcgcggcc tcggggaagg agccgttcgt ctaccgcggg gagttcaagt cgggaaccgg
     5221 caacatgcgg ttcgtggcga ccgtgcggga cgtcacctcg aaccgcgtgg gcgtgggctc
     5281 ggcaagcgta cgggtcgagt aggtctcact tcttcttcgg agcggcggcg ggcgccggag
     5341 ccggggccgc gctccccacg accgagaacg gcgcgacgag gtcgagcttc ttctgcgtga
     5401 cggtgtcgag gatcgtgacg cgcagctcgt agtcgttcgg cttgaggtac tggccgaggt
     5461 tcgactcgat gacgccgccg gcgaggtcga gaacgagcgc gccgccttcc tgcgacgggg
     5521 gaaccttctg cggctcctcc gggggggtcg gcacgtcgat cggcggctgg cccttcggct
     5581 tgatcttgat cgtgcgcctg agcgtgagcg tgcgcgacac cgggtccacc ggggggttgt
     5641 agatgcggac cgcgtacgag aggccatcgg cggggtcgag cttcccgtcg cccttgacga
     5701 cgaagcgtcg cgccgagaac gtgaacgggt cgtcggccgc cggggtgtcg gtcgggaagt
     5761 cgttcacggc gaccacgagg gccgaggccg agaactcggt cggcagcgcg gggaccgtga
     5821 tcggccggcg ccccgagacg aggaccttgc cggcggcgtc gaggaggacc gcggccacgt
     5881 cgtagtcgcc ggggagaacg cgcagcgcgc ggtcggcgaa gaagtcggaa cgggtcttcc
     5941 ggagcttcgc gtcctcgtcg acgcgggcgg cctcgcttcc gtcggccgcg cgcgcgacga
     6001 tcgcgagccg cacgccttca cccgtcccga ccgcggcggc cggcgcatag acctggacgc
     6061 tgaggcgcgt cgccccgtcg cgataggcga gcgagaggag ggcgaggggg ccgaaaggct
     6121 ccttcgggag tccgtcgatc gcggcgcgcg acgccggaga gagggccggg cccttcgcgt
     6181 cctcggccag cttcttctgc tccgcctcga cctgctcgcg cgtcttgtaa acggggactt
     6241 ccttgagatc cgggctcacc accgccacct gtgccgcctt ggtctcgagg cgcctggcag
     6301 gtgacatgtc gagaagcgac tcggtcccga ggcccacgtc cacgatgaac ttgatctcct
     6361 gggactggac gtcggaccac gcgggcaggt gcgacttgtc gtacacgaac gtgtagagca
     6421 tcgccgcgcc gccgcccgtg ccggtcacgc cgccggcgct tcccgttccg aacggcgtgt
     6481 ccgcggacga cgtcgccttc gccgtgatct ccttcggagg gccgatcagg atgagcgccc
     6541 ggcctcgctc ggtcagggcg ccccgtttct tcccgagcgt aaaccgctcg tcggcgagct
     6601 ttacgagcgc gtcgaagcgc tcgcggaact cgttgcgcgg cgtcttgatg tccgggtcgc
     6661 gccgcgccca gaagagcgcg atgaacttct cggcatcctc gtcggaggcc gccttcttcc
     6721 agtccttctt gtcggcgtcc gtcgcgaggt actggtactc gggcgacttt tcccagtcct
     6781 tgtacttgtc gagcgcggcc gccgcgggcc ccgccgccga gacgaggagg aggaagagga
     6841 cgagggcgga ggtcgatcgc ttcattgacg actccagcgc gcgccgcgcg gtttcagtcg
     6901 ggcacctcga ggaggcgcac caacccgcga ctatacagga gcgctcgcct tctcccgacg
     6961 acggacgagg cgatggcgtc tcagctacgg aaacttggaa ccgaatcccg gatgccgttc
     7021 gcgactcgta acagccctcc ttcagccgat ggagaaagca tttgtttgta aagggataaa
     7081 agccggattg caggcgatgg gaacggcatg tagagtgcta ggaccattta ggccgaactg
     7141 cactcgaagc tccgtcccag gcgccgggga ttcatcccct tggtcagccc gccgcccgga
     7201 acggaaaatc aattcgaatt tatttggagg aggaaggaaa agcatgacca ggaaaaggat
     7261 cctcggactc ctggctctgc tcctcgtggt cgtcgtcggt gcgacgagcg cgatgggtca
     7321 gagcacgacg acgggcaacc tcaccggaaa ggtcgagaac gagaacgccg gcctgcccgg
     7381 cgtgaccgtc acgatcaagt cgcccaacct gcaggggacg cgcacggcga ccacgtcggc
     7441 ggcgggcgcc ttcacgttcg cggcgcttcc ccccggcgag tacaccgtct cgttcgcgct
     7501 ccagggcttc cagtccatca cgaagaccgt caaggtgacc gcgggccagc agtcggccct
     7561 cgacgtcaac atgacgctct ccggcgttca ggcgagcgcc gtcgtcacgg cgaagtccga
     7621 catcgtctcg acgacgacgc aggcctcgac gacgatcacg aacgacctct cgaacaccct
     7681 gccgaatacg cgcacgatcg cccaggccgc ggcgctctcc tcgggcgtca ccccggtcgt
     7741 gagcctcggc aacgccctca cgatcggggg cgcgcccacg ttcgacaacc tttacacggt
     7801 cgacggcgcc gtcatcacgg acaacatccg cgcgacgccg aacaacctgt tcgtcgagga
     7861 cgcgatccag gagacgacca cgaacgtgaa cgggatctcg gccgagtacg gccggttcca
     7921 gggcggcgtc atcaacacgg tgacgaagtc cggcggcaac acgttctcgg gctcgttccg
     7981 cacgacgttc gtcaacgacg cgtggagcgc cgtgacgcct cagggcgaaa cgcgcgtcca
     8041 ggatctccag gagcggtacg aggcgacgct cggcgggccg atctggaagg accacatctg
     8101 gttcttcggc tccggccgca gcgcgaaggt gggcacggcc cagacgacgt tcgcggacgc
     8161 gaaggtgggc ggcgccacgc ccgtcggcgg ctacgcgggc attccctaca cgaacacgct
     8221 cgacgagcag cgctactccg gcaagctgac gctcaccccg atccagaacc acacgttgac
     8281 cgcttcctac ctgagcatca gcaacaagga agggaacaac gtcttcggcg tcgtcctgga
     8341 cgagaagagc ttcgtccagc gcgagctgcc gcaggacacg ctctcgatcg cctacaacgg
     8401 cgtcttcacg tccaacctct tcttcgaggg catgtactcg aaccgtcact tcacgttcaa
     8461 gaactcgggc tccacgttca cggacgtcat caacggcacg ctcatgcgcg accgctcgcg
     8521 cggcaatgcc cggtggaact ctccgacgtt ctgcggcgtg tgcggccccg agaagcgtga
     8581 caacgaggac gtcctcctca aggctacgta cttcctctcg acgggctcgc tgggctcgca
     8641 caacatcgtg ctcggctacg acacgttcaa gggcatccgg acctcgaaca actaccagtc
     8701 cggcagcaac taccgcatct tccacacgag cacgatcaac tacaacggca cgatttaccc
     8761 cgtcatcaac aacgtcggga cgtcgtcgtt catctactac acgccgatct tccagctcac
     8821 gcagggcacg agcacgaaga cggactcgtt cttcctgaac gaccagtggc gcctcaacga
     8881 ccgcctgtca ttcaacctcg gcgtccgcta cgacaagaac aacgccaaga acagcgtcgg
     8941 cgcgacgacg gcgaacgact cggcgtggag cccgcgcctc gccgtgaact gggacgtgac
     9001 gggcaagggc aacgtccgcc tcggcgcgag ctacgcgcac tacgtcggcg gcctgcagga
     9061 ttcgatccag gactcgacgt cggccggcgg ccagcccgca acgttctact ggtactacaa
     9121 cggcgccgga acggccccga tcaacgtggg cgtcccggcg ggcacgcagc ccccggtctc
     9181 ggccgctcag gcgatccagc aggtcttcaa ctggttctac gcccagggct gccctgacct
     9241 cgcgacgtgc aagctcccgc tcgcctacgg cagcatcccc ggcctgaacc gcgcgatcga
     9301 cggaacggtc aagtcgccgt atgcgaggga gtacacgatc ggcatcgcgg gcaacatcgg
     9361 ctcgagcttc tcgtatcgcg ccgacgtcgt ccgccgcgag ttccacgact tctacaacct
     9421 ggtcgtcaac acgcaaacgg gttacgtgac gaccccgacg ggcgccaagc tggacttcgg
     9481 cctcatcggg aacacgaaca gcgtcgagcg caactacacg ggcctccaga cgcagttcca
     9541 gtaccgctgg cagtggctca cgatcggcgg caactggacc tggtcgcaca cgctcggcaa
     9601 cttcgacgcc gaggccggga actccggcgc ggtcacgaac gcgaccgagg cgtaccccga
     9661 gtacaagcag atgtcgtgga acgccccgta cggctcgatg gccgcggacc agcggcaccg
     9721 ggtccggctc ctcgcgtccg ccgacctccc gttcatcccg aagtcgctgg gcaacttcaa
     9781 cctcggcctc atccagggtt acgacacggg ctccccgtac ggcgcggtgg gcacggtccg
     9841 cgcgtccaac tacgtgacga acccgggcta ccagcagcgt ccctcgacgt ccacgtacta
     9901 ctacaccgcg cgcgacgagt tccggacgga cgacatcaag cgcacggacc tctcgctcac
     9961 gtggacgctc cggctcttca acaccctcga gctcttcgtc cagccgcagg tcctcaacgt
    10021 cacgaacaac cagggagcga tcgccgtcaa ccagacggtc cagacgctca acaacacgac
    10081 gggctacctc cacttcaacc cgttcacgac gacgcccgtc cggggcgagc gcaacgtggc
    10141 gaacccgacc gcgaactgga attacggccc gagcttcggg caggtcacga gcgtcgccgg
    10201 ctggcagcag ccgcgcacgt tcctcctcac ggccggcctc cgcttctagt cttccgcccc
    10261 gtcctttcgg gagccgggcc ttcgggcccg gctctttttt ttcgcccccc tgggttaccc
    10321 tccctcgatg aagtccggaa ggcccgccgc ggccgtcctc gcctgcgcga tcgccctcgt
    10381 ctccggagcc gccggctgcc ggcgcgggcc ggcggccgcg gccttcccgg gcgctcccgt
    10441 cattctcgtc agcgtcgaca cgctccgctc ggaccacctc ccggcctacg ggtacaaggg
    10501 cgtcgagacg cccgcgctcg acgcactgcg gaaggactcg atcctgttca cgcgcgcgtg
    10561 ggcgcacgtc cccctcacgc ttccctcgca cgcgtcgatg ttcaccggga gggagccctc
    10621 cgcccacggc atccacgaca acctcggtta ccggctccgg gcggacgtcc cgactctcgc
    10681 cgagctcctc aagaaggagg gctacgagac cggcgccgcc gtctcgtgct tcgtcctgaa
    10741 gggggccgcc agcgggatct cgcgcggctt cgacttctac gacgacgccg tggaaccggc
    10801 ggagggggcg gcggccctcg gccgcgtcca gcgggcggga cccgagacgg tggcccggct
    10861 cgagacctgg ctcgcggcgc ggccgacaag cggcaagacg ttcgcgttcc tccacctcta
    10921 cgagccccac acgccgtacg agccgcccga gccgttcagg acgcgctacg ccgcgcagcc
    10981 gtacgacggc gagatcgcgg agtccgacgc gatcgtcggc gggctcgtcg cgttcctgaa
    11041 ggcccgcggc ctctacgaca gggccgtgat cgtcttcctc tcggaccacg gcgaaggcct
    11101 cggcgagcac ggcgagtccg agcacgggat gttcctctac cgcgagagcc tgcaggtgcc
    11161 gctcttcgtc aagctgccgg acagccggcg cgcgggcgag accgtcgacg agcccgcggc
    11221 gctgttcgac gtcttcacga cgatcgccga aggcgcgggc gcccggggct tttcgcgccc
    11281 cgagggcacg cggtcccttc tcgacccgcc gggggctccc gcgcgccgca tcctctccga
    11341 gacgttcttc ccgaggatcc acttcggctg gagcgagctg tcgtcgctct tcgacgggcg
    11401 ctggcactac atcgaggccc cgaagcccga gatctacgac ctccccgccg atccgggcga
    11461 attgaagaac ctctatgccc agaagcccga cgcgctccgg ccgatgcttg ccgagatcgc
    11521 gaagcgcaag ccggtgtacg aatcggccgg cgacctcgac cccgaggcgc gcaagaagct
    11581 cgcctccctc ggctacctga gcgcgggccc gtcgagcgcg aacgcggggg gcctcgacga
    11641 cccgaaggac cggatccgga ctttcgagga gctccgggtg ggcctcggcg acctcacggc
    11701 cggcaattcc gcgaaggcgc acgcgatctt cacgaagctc ctcgcggaga acccccggat
    11761 gctcgacgtc tgggacatgg actcgaaggt cctgatccag ctcgggcggc ccgaagaggc
    11821 gctcgcggcc ctcaagaaga cggtcgatct cgcgcccgag gcggcgcgga cgccgtacct
    11881 gcgcgaggtc gcgaacctct gcctgcagct cgggaagtgg gacgaggcgc tgaaccacgc
    11941 cgaggcgctc cgggcgctcg gcgacccgga cgcggaagac atcgccgccc gcggcttcct
    12001 cggcaagggc gacctcgccg ccgccgggcc cgccgcccgc gccgcgtacg agaagggatc
    12061 gggcaagacg aaggtgcgcg gcgcgctcgt cctcgggcgc ctcgcggtca tgcgcggcga
    12121 cgtcgcctcg gccgacaagt ggtcccagga ggcggcggcc ctctccgcgg acgacaagct
    12181 ggtccagtcc ggactccaca tgctgcgcgg cgacgtcctc gcccggcagg gccgggcgcc
    12241 tgaggccgag aaggagttcc tcgaggagat ccgtctctac cccggccgca tggacgcccg
    12301 cgtctcgctc gccgccctct acgcgtccgc gggccgccgc gaggacgcgc gccggacgct
    12361 catcgcgctc gtcgtccacg agcccaagcc ggaggctttc ctcctggcca tgcaggcgtt
    12421 ccgcgcgacg gaggacccgg acggcgagcg cgagatgcgc cgcgaggccc ggcgccgctt
    12481 cccgcgcgac ccgcgcttct gaggcgttac ttcttcggcg ggagcgtccc cgtcacgaca
    12541 aacgtcgtcg cggcctcggc cttcttcttg gagacgttgt ccgtcacggc gaccttgatc
    12601 tcgtagtcgc cgggcttctt gaggtactcg ccgaggcgcg tctcgatcag gacggccgag
    12661 acgtcgacgg tcaggatccc cccggaggcc tggtccttgc ggtcgggtgc cggcgtcggc
    12721 gggtcctgcg gctgaggaac gtcggaccat tgcgcgccct tcggcctgaa ccggaccgtg
    12781 cgcgaaaggc tgatggtctt ggtcgcgggg tccacggccg ggttgtagac gcgaaggacg
    12841 aaggacaggc cgtccgtcgg gtcgaggcgc gcccccttgg agatgaagcg gtggcccgag
    12901 aacgtgaacg cgtcctcggg cttcggtttc ggcacgatga agaacgcgct cgcgatcacg
    12961 aggggcgaga ccgcgaagtc ggtgggcacg ggcgcgacgg aaacgctctt ccgggccgcg
    13021 gcgacggcct tccccgaccc gtcgaagatg ccggcggcca ccgtgtactc gccgggcggg
    13081 accgagaacc accggctcgc atagaggccg ccgcccggcg ccggctcgag aggcgtcgat
    13141 tcctcgtatc gcgcggcgtc cttgccgtcc ggggtccgga cgagaaaggc gaggtggctt
    13201 ccggccggcg gctcgcccgc ggccgcggtg aggaagagct gggcctggat gcgcgtgtcg
    13261 tccgtttctc cgccggccag cggaacgacc gcgagaagcg cggaatcctc ggcgaggccc
    13321 tcgagcgacg tccggatcgc cggcgtcagc ggcggaccct tcttcgcctc cgcctcggcg
    13381 gcgagcgcgg cctggcgctc ggcctcgagc tccgcggccg tgcggtagtg cggcgcctcc
    13441 ttgaggtccg ggttcttcag gagggcggcg cgggcgttca cctcgaggcg cttgacgtcg
    13501 ccgctggacc ttcccgccac gaaatcgctc gcctgctcca cgacgaactc ggcggtgagg
    13561 gatttcatct tcgcccactc ggggagctgc gccgcctcgt agatgaacgt gaccttgctc
    13621 tcgccgatcg acgtcccggg ttcgttcggc ggcgggatgt tcgccgagcc cggggccgcc
    13681 acgggctgcg ccttcgtccc ggcggaccgc tggagcgtct tcggggggcc gagcaggatg
    13741 tacgccctcc cccgctccgt cagcgcgccg cgctgccgcg gcatcgcgaa gagctggtcg
    13801 gcctccttca cacgctggtc gaagccgagc ttgtactcgt tgaccggcgt cttgaggtcc
    13861 gggtcgcgcc tggcccagaa gagctggatg aaccggtcgg cctgctcgtc cgccgtgatc
    13921 ttcttccact ccttctgctc ggcgtccgtc gacaggaaga cgaactcggg cgacttcgtc
    13981 cagtccttgt agcgctcgag gccggcgccg aggcccggga cggacgcggc gagaaggaga
    14041 agagcgagaa cgcgaatgga ggttttcatc ttgaccgatg gaataccgca aagagacacc
    14101 cctgacaagg gcgaagcggg gcgcctttcc gggcgcctga tgcatacgca tcagcccgtg
    14161 cgagcccctc gctctcatag gatcatcccg gaaactccgt cccggcccga tgcgatttcg
    14221 tccccgatct acggtctttc gtcatcttca catttcccgg cgagcaggcc ggggtacccg
    14281 aaggagggaa cccatagtga gcatttcaac gacctcgacc aacgcaaggc gactttcgat
    14341 cctcgtcggc gtcgctctcc tcgtgggcct cgccctcgcg cccgtcgtcc tcgcacaggg
    14401 gcttccgacc gccaccctgt cgggccgcgt gcgcaacgac gcgctcgatc tgccgggcgt
    14461 caccgtcacg gcgaagtccc cggccctcca gggcacccgc acgacggtca cccgcgcgaa
    14521 cggcgactac gtcctcgcga acctcccccc cggcgactac cagatcacgt ttgcgatcca
    14581 gggcttcaag acgcagacgc tgtcgaagac gctcggcgcc tcgcagcagg cgaccctcga
    14641 cgcgaacctc gcgatgacgg tcacggcggc gacgacggtc acggcccaga gcgactcgat
    14701 ctcgcagtcg cccgcccagg cgacgacgta cacgggcgac gtcctcagca agctcccgac
    14761 ggcgcgcacg gtcacagcgg ccgtcaacct ctcgcccggc gtcaaccaga acggcccgaa
    14821 cggggcctcg atctcgggcg cgcagtccac ggaaaacctc tacacggtca acggcgtcac
    14881 gatcacggac aacgtgcgct cctcgccgaa caacctcttc atcgaggacg ccatccagga
    14941 gacgacgacg acgacgtcca gcgtctcggc cgagtacggg cgcttcaccg gcggcgtgat
    15001 caacacgatc acgaagtccg gcggcaacgt gttctccggc tcgttccgtt cgacgctcaa
    15061 caacaacgcc tggaactcca cgtccggcta ccggacggcg acgggcgtga acccccagga
    15121 aggcacgttc acgaacacgg tcacgtcgac ctacgaagcc acgctcggcg gcccgatcct
    15181 caaggacatg ctctggttct tcggagccgg ccgctacttc gacacgagcg aggccctcac
    15241 ctccgtcacg cgcttcacga acatcccgta cacgaacggc gcgaaggagt tgcggtacga
    15301 aggcaagctg acgttctcgc ccgtccagaa ccacaccctc acgggctcct acatcggcgt
    15361 caagcacgac gacgtcaact actacttcac gagcatcccg atcgcggacc tcgcgagcat
    15421 ctacgaccgg cagctcccgc aggagctcat ggcgttcaac tacaacggcg tcctctcgtc
    15481 gaacttcttc ctcgagggct cgtactcgaa gcgcaagttc acgttcgaga actccggcgg
    15541 ccgcttccag gatgtcgcca acgggaccgt catccgcgac ctcgggctcg gcatctcgta
    15601 caacgcgccg atcttctgcg gcgtgtgcgg cccggagaag cgggacaaca acgagttctt
    15661 cgtcaagggc acgtacttcc tctcgacgcc gggcttcggc tcgcacaacc tcgtggccgg
    15721 ttacgacaac ttcgccggcc agcggctctc gaacaactac cagtcgggca gcaactacgt
    15781 catctacacg ttcggatccg ggagatcggt cttccagggg cagaacgtct accccgtctt
    15841 cgagcccggc ctcaccgagc tcgactactg gccggtcctg caggcttcgc agggcagcga
    15901 cctccggaca cagtccgcgt tcctcaacga cgcgtggcgc ctgaacgaca ggctttcgtt
    15961 caacatcggc gtccgctacg acaagaacga cgccacggac gcggcgggca acgtcacgtc
    16021 gaaggactcc aagttcagcc cgcgtctcgc cgccacgtac gacgtcaccg gcaacggcag
    16081 cctgcgcctc acggcgagct acgcgcagta cgtcgcggcc ctgcaggaga cgcaggcggg
    16141 cagcggcgcg acgctcgccg gcagcccggc cgacttctac tggtactacg acggcccggc
    16201 catcaacacg ggggcgggcc cctacctcac ctcgcagcag gccacccaga agatcttcga
    16261 ctggttcgcc gccaacggct gcctcccgaa cccgctcgcc gcgagctgca aggtcccgca
    16321 gggcggcgcg ccgtccatcg gcggcgtgaa cgtccagatc cgcgactcgc tggcctcgcc
    16381 gaatacgaat gagtacgtgt tcggcatcgc gggcaacttc ggcaagtcga cacagggcag
    16441 ctaccgcgtc gacttcgtcc gccgcgagta caaggactac tacgacctca agaaggacac
    16501 gacgaccggc aaggttgcga gccccttcgg cgacatccag gatctcggtc tcgtcgtgaa
    16561 ctcgagcgac taccgccgcg agtacaccgg cctccactcg caggtcttct atcgcttcac
    16621 cccgagcttc acgctgggcg ccaactggac gtgggcgcac ctgatcgggg acatcgtcgg
    16681 cgagacgagc ggctccggac cggtgcgcgg cggctcgcac gtgtaccccg agtacgtgca
    16741 gcggtcgtgg aacaaccccg tgggcgacct gagccaggac cagcgtcacc gcgtccgcgt
    16801 ctacggcaac tacgaccttc ccgtgccgcc gagctggggc tccttcaaca tcggcttcat
    16861 tcagacctac gacacgggca cgccttacgg cgccgccggc acgatccgca cgggctccta
    16921 cgtcacgaac cccggctacc tgacgcctcc cgcgtccgtg acctactact tcacgccgcg
    16981 cgacgcgtac cggaccgaga ccatctggcg aacggacatg cagctctact attcgcaccg
    17041 catcgtcggc ggcctcgaga tcttcgtgat cccgcaggtg ttcaacgtgt tcaacgcgca
    17101 gcacatcacg gccgtgaaca cgaccgtgaa cacgaacttc aacacgtcga acctcctgcc
    17161 gttcaacccg ttcacgaacg cgtccccggt cgagtgcccg caggggacgt ccggcgccgc
    17221 ctgcaaggcg atgggcgcca actggcagaa gggctcgctc tacggcacgc cgaccgcctc
    17281 ctccagctac cagacgccgc gttacttcca gctttcgctg ggcctccgct tctagcgttt
    17341 tccccctcct tcctcgaggg gccccggcgc tgccggggcc ccttttcttg tccgggcgct
    17401 agactcgccg cgtgttctcc acggcgcgaa ctcgcaccag gctgatcgcg gccctcggga
    17461 ccgtcgtcct cgccgccggc tgcggccggc cccgttcctc ggcccgcgtc ttcccgaagg
    17521 cgccggtcgt cctgatctcg atcgacacgc tccgctccga tcgcctcccc ttctacggct
    17581 acgccggtgt gaagacgccc gcgctttcgg ccctgcggga cgattcgatc ctcttcgaga
    17641 gcgcctggtc acacgcgccg ctcacgctgc cgtcgcacgc cacggtcttc accggcctct
    17701 tgccggccca gaacggcctc cacgacaacc tcggctactc gctgaatccc tcggtcccga
    17761 cgatcgcgga gctcctcggg aaggccggct acgcgaccgg cggcgccgtc acgagcatcg
    17821 tcctctccgg gacgaccggc atcgggcgcg gcttcggcct ctgggacgac gatgtcgtcc
    17881 cgacacgagc cttccaggcg ctgagccgcg tgcagcgttc cggggacgag gccgaggctt
    17941 cgatggagcg ctggatcgag aagcagtccg ggccgttcct cgccttcctc cacctctacg
    18001 agccgcacgc cccgtacgag ccgaaagagc ctttccggag cctctacgcg agcccgtacg
    18061 acggcgagat cgcgaccgtc gacgcgatca ccgggaagtt cctcgacttc ctgaaggcga
    18121 agggcctcta cgaccgctcc ctgatcgtcc tgatgtccga ccacggcgag gggctcggcg
    18181 accacggcga ggccgagcac ggcgtcttcc tctaccgcga ggtcctgcag gttccgctcc
    18241 tcgtgaagct cccgaaaggc gatgccccgg cgccgcgcag cgtggccgcg cccgtgcagc
    18301 tgtccgacct cttcacgacg atcggaaagg ccgtgggact cgagggcttc gccccgcccg
    18361 cgggcacggt ctccgtgatc gacctggcgg acaccgcccc gcagccggcg cggcggatct
    18421 acgccgagtc gttcttcccg cgaacgcact tcgggtggag cgagctgcgc tcgctcgtgg
    18481 acggccgctg gcattacatc gaggcgcccc ggcccgagct cttcgatctc gtggccgacc
    18541 cggctgagaa gtcgaacctc gcggacggac ggcccgagcc gttccggaag ctgcggatcg
    18601 agatggaggc gctccggacg tcgttcgcgg cgcctgccgc cgccgaccac gagacggcga
    18661 agaagctcgc gtcgctcggg tacctcagct cgggccaggc cgcgggcagc ggcccgctgg
    18721 acgatccgaa ggaccatatc gcgaccgtcg gcctcatgaa ggaggcgctc cgcgagttcg
    18781 tcgagggcag cgcggcgaaa tccgtggcga tcacgcagaa gctcctcgcg gagaaccccc
    18841 gcatgctcga catctgggag ctgcagtctc aggcgctcgc gaagcagggg aagaccgacg
    18901 aggctctcgc atcgctccgg aagacgatcg agctcgcacc cccgggctcg acgcagtacg
    18961 tcgtgtcgtt cgcgaaccac gcgctccaga tgggcaaggc cgaagaggcc cggaagcacg
    19021 ccgagctcgc gcggtcgatg ggcgatccgg cgggcgacga gatcctcgcg cgcgcgtgcc
    19081 tcgcgctcgg cgacctcccg gcggccgagg cggccgcgcg ggagtgcctc aagagcgacc
    19141 gcaaccgcga caaggggctc ctcgtcctcg cgcaggtcga ggcgcgccgg ggcaacctgc
    19201 cgaaggcgct cgaggcgtcg aaccgcgtcc aggaaggcgt cgtccacgcc ctcccgctcg
    19261 caggcctgca tctgctccgc ggcgacgtcc tcgcgcgcat gggccgcccg aaggaagcgg
    19321 aagcggagtt ccgcgaggag ctgcgagcct acccgacgac gctcaaggcg tggtcctccc
    19381 tcgtcatcct ttacgccacg cagaaccgga cggcggatgc gcgcaaggcg atcgaggaga
    19441 tggtcgcggc cgccccgggc gtcgacgcgt acctcgccgc gtaccagacg ctgtcggtcc
    19501 tcggcgaccg cgccggcgcg gagcgctgga agcaggaagg cctcaagaag tacccgggcg
    19561 acgcgcggtt caaccgcccg gcgcgcccgg cctgattcca gccctcagcg tcccgggagc
    19621 ttcttcccct cctcgagcca ggccttcagt cccccgagca tccgctccca gccgggtccc
    19681 agctggtcgc ggtacgactc ccacacgggc ccttcctcga tgccctcctg gacgaccgtc
    19741 acgtcggtgc cttcctccac ggcctcgaac gtgacgacga ggcgcatggg gccgaaggac
    19801 agcccttcgg ggctcgagaa gacgaggttc gagaccgcga gcgtgcgccc cggctcgaac
    19861 gtctcgatct gccccatgga gctgtagccg gcgtccgagt cggggtccgc gtaccacccg
    19921 agcgcccagt tgccgcccgc gcgcgcgccg atcacggcgc cgtccgcgag ccagctgagc
    19981 acctcggaga cccgcacgaa ggcctcgaat gcgcgctccg gcgcgacgcg cacgaaaacc
    20041 ccgcgtgtca cggagtcgct cacggccgcg atgctacgac atgcgagaat cgccgccgcc
    20101 atgctgatcg gactcaagag cgcgctcgag cgcgccatcg tcgacgcctg cgtcaagacg
    20161 ttcggggtcg agcccccgcg cctcgtcctc gagacgcccc cgaaggtcga gctcggcgac
    20221 ctcgcctgcc ccgtcgcctt cgagctcgcg aaggtgctca agcgcccgcc gcgcaagatc
    20281 gccgaggagc tcgcccccgc cctcgtcctc ccggacgtcg tcgtgaagcg gaccgtggag
    20341 ggcggcggct acctgaactt cttcgtggac cgcccgaagg tcctccgggc gatgctcgag
    20401 gacacgcacg acgcgcagtc ggcgggggcc gcggacgccg gaaagctcat cgtcgagcac
    20461 acgaacatca acccgaacaa ggccgcccac atcggccacc tgaggaacgc cgtcctcggc
    20521 gacgtcctcg tccggaactt ccggcgcctc gggaggaacg tcgaggtcca gaactacctc
    20581 gacgacaccg gcgtccaggt ggcggacg
//
LOCUS       DAOMFY010000041        20537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_831_length_20537_cov_8.832243, whole genome shotgun sequence.
ACCESSION   DAOMFY010000041 DAOMFY010000000
VERSION     DAOMFY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20537
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_831_length_20537_cov_8.832243"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1241
                     /locus_tag="PLB01_05490"
     CDS             141..1241
                     /locus_tag="PLB01_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQQ76787.1"
                     /translation="MNAAPTVAPAAPAKLRKFPCAACGADVVWSPGAAALTCPYCGAK
                     KEVPKTPGEVAERPIEEALKGATDLGWGLARKAVSCRGCGATTTFDAGVAASRCAFCG
                     APSVVEAPPSTTMVRPAGVLPFKVDRNAATGRFRQWLSSLWFRPDDLQQKSALSELKG
                     VYVPFWTFDAATHSAWTADAGYDYQVEVQVEENGQWHTRYETRTRWEPASGVLEHFFD
                     DLPVAASRGLPPDLARAIEPFPTADLLAYEPSYLSGFLAEEYAVGPKEALASGQQRMT
                     QELYALCGREVPGDRFRNLQVRTAWSGLAFKSGLFPVWIAAYQYGGKPFRFLVNGVTG
                     KTDGHAPWSWVKIGLAVLAAILLFIILAAIAG"
     gene            complement(1261..2301)
                     /locus_tag="PLB01_05495"
     CDS             complement(1261..2301)
                     /locus_tag="PLB01_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76788.1"
                     /translation="MKPMARLFPLALGSLLTALTGCSGMYTTAIATPGSVPAPPAGAP
                     AVTLVVSDRRPPKEGGDDPSRVGSIRALAGNSAGMREQSPDAVRRLVEAATKSGLAKA
                     GVGTADAGPTLEVAISKFWMDGYSSYTAELWGTLRARRDGQDVWSRDLKVNATGEQPG
                     SPSEMFNRVWGWALDKWRDEVARAMAEPGALAALGVKPPAAVAPAGAQGLGTLGPVGL
                     SVASEFNVDDRQKVTFEIKSRDALKQAGLTEKRGSFPEVAVRYVSVYEKNAKTGNVIG
                     YRVTTRLSLKDDGGRILWEDSFVVKGTLGDLDPKNVNYVDASFSTSVDYKWTTEVVRR
                     LKSEAFRKAAGR"
     gene            2408..4096
                     /locus_tag="PLB01_05500"
     CDS             2408..4096
                     /locus_tag="PLB01_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76789.1"
                     /translation="MGSMTEARPPASRLARADSRPILVTALVVGWILLCGLGALRVLV
                     VLGSSAASSALGTSDPLARAAAAAPDLVSDETRQVLAQREQRASLDRALAVPLLLVST
                     LGIVGAVALLRRRTWSRALLLTVGLLAIGISAFHAYRSVGIAAAPAAGLEAEPDAQNA
                     LALLRAAAGIGLALQSIPLVVAMSLLRHPIVRDYLGVETRPRSGPDPLLVVAACAVLV
                     AAGAVLFSKSRAPAPAPAKSAPAFHPPEPAETFRWSDQPITFSPPAGPWTRERHAEGG
                     RKGVSFTRYATPPSRIIVAEAFVEIPPRTVDEVLPRFRLTKEKFPSVDSITVGEPAPA
                     AVAGHAAFQTDYTMRERSMQHRGREFLTVVSGHVFVFTLLGPEADIRTFENLVASVRF
                     TGADEAGGAAASPAAAAAASPDEPPGGDVTVVRVGERRVIVRIPAGWERVDYDKRQEF
                     QRGEARFALFDAGPAQPGPAGEVDDPDLVRRALNTIGHDPARREVASRTPTQIGGRDA
                     LAVDTWEPLSHAYRERFYFVLNAGRLLVAAPVMGRHEDTKAPLEALVRSIRFVD"
     gene            complement(4093..5097)
                     /locus_tag="PLB01_05505"
     CDS             complement(4093..5097)
                     /locus_tag="PLB01_05505"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQQ76790.1"
                     /translation="MASVRCGRSTERAAVRDASGGWEEVRIGTDAVFSGLHFVDPSVG
                     WIVGGSPFVDGGIVGRTEDGGRTWRYVTGVTRGGRTSGLGAVHGFDRMRACAVGDGVF
                     LTFDGGASWQRATAPGARFLSALDFRDGNEGWAAGATGVIHTADAGLTWTVVDGKDSP
                     ARRANGRVLHFTDGRSGWLAGKDGVLLRTRDGGATWARVPIPAPANTEHPPDLWGGTW
                     PDASHGWIVGEYGTVLRTTDGGDTWTLVDAGTRDAFLTGIAFAGADGWIVGFLPNNAA
                     RSVVTRTRDGGATWALERTLDGEELRAIQALDADTAWAVGDRVRTEPQRMLRRAGRPG
                     "
     gene            complement(5183..5653)
                     /locus_tag="PLB01_05510"
     CDS             complement(5183..5653)
                     /locus_tag="PLB01_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HQQ76791.1"
                     /translation="MNRSREWLGLVLWILLCEGTGFIASQWKPGAWHAALVKPAWNPP
                     NGLFAPVWILLYALMGIAAWLVWRSAGTPGRRAALGLFLVQLALNGAWSWIFFGRHAI
                     GAALVEIALLWLAILATLLAFGRVSRPAAWLLAPYLAWVTFATALTFAIWRLNG"
     gene            complement(5700..6185)
                     /locus_tag="PLB01_05515"
     CDS             complement(5700..6185)
                     /locus_tag="PLB01_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HQQ76792.1"
                     /translation="MTAALWILAVLLVLAGLAGTVVPALPGAPLVFAGLLVAAWIDGF
                     ARVGPWTLAVLGVLTAATIAVDVLSTALGAKRAGASKGALVGAAIGTVVGLFLGLVGV
                     LAGPFVGALIGEFAVRRDWRQAGKAGVATWIGLAVGTAVKIAIVFAMLGVFALAYLFS
                     R"
     gene            complement(6200..8374)
                     /locus_tag="PLB01_05520"
     CDS             complement(6200..8374)
                     /locus_tag="PLB01_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HQQ76793.1"
                     /translation="MTTRLKKVAGGALFVLVTALAAGRAAAATSGEQDADSRTWTVGN
                     TLLSTSLRYTNEGRIVTMGLRDRSRGLDWASSEGGLGFRVVLRVPSPDAPAGADPAGF
                     DPVTLTEASTWDLVRATSKPSSDGAVRFDLVLASREAPVSVTWSLVSYTESPVFRSSV
                     AVTNTGTQPVLLEAVDGLDAGLTAAGGTLTSLTVNNFNWGHPATPFHTSQATLKPGSV
                     VQARTGPFGNQAAWLALRSAKWNAGVFAGWEWSGPGRLAAATAADGSTSLGVGFAPAT
                     FGHVLAPAETFSSPTAFLGTFSGNLDAAGAATRAFVTRRIAPPLPAPDFPWAGFDTWG
                     YSYGIVEPQVEDLIDRAAWLGVEVFTVDAGWYPRMGDWVADALKFPGGLKNLAARAHG
                     KGMRFGLWIALGAADPLSQVVLEHPDWLARVGGETVATDMDGVALCLADPRVRAWILA
                     QVDRLATEGSLDWLVHDFTVITGCDAPGHSHQAGDGWWASTAGYYAVLDEIRKRHPKL
                     VLENCWDGGALFDFGMVARHDTSATSDRNDAYGNQRAIYGGTYLVPPRYLDKYVGDDG
                     SPDTYRFLSALPGGPFLLMGRIADWTQETEEAAKAAIALFKQKRVVHRDGAIYHLTAQ
                     PGEGPIAAVESYESKSGAGTIVVWSTPGTAPAPGTNAALRVLPVGLTASALYDVAVST
                     SLPAVRAPQALPTDTGANLMSKGITLGIAPADGAAVVTLTKR"
     gene            complement(8631..10856)
                     /locus_tag="PLB01_05525"
     CDS             complement(8631..10856)
                     /locus_tag="PLB01_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQQ76794.1"
                     /translation="MNVRSGRLVLVSVLVLLAAGALADEGMWMPQQIPQLAPRLKEMG
                     FQGDPQAFADLTGQPMGAIVSLGGCSASFVSPDGLIVTNNHCVQGALQYNSKPDRNLM
                     VDGYLAKTRADELWNGPGSRVYVTVSVKEVTDEIAGRVDAKLTDRKRFDAIEQRVKAK
                     TAECEKGGLRCSVSPFFEGLKWFELAQMEIQDVRLVYAPPAGIGNFGGETDNWQWPRH
                     TGDWSFYRAYVSKDGKAVPFSKDNVPFKPKHWLKVSPAGANPGDLVFVAGYPGRTARL
                     NPYEDVKETVEWTLPRSIRRSTDRIAILEKLGKDDKETALRVAQAIRGLNNGLTKNKG
                     VLQGMVKGGLLADKERMEKDFVKWIDADAARKAKYGDVIPAINALTAERVKTRERNAL
                     LTELGGSGGMMVGSALGASQILYRYSIEKAKKDADRDPAYQERNWTRLRESQERAQRT
                     LSPKADRALMAYALADVAKLPAEQRIDVLDKEIGLKPGMPEADAGKAIDAWLDRTIAG
                     TKIYDKDFRMSLFEKSASDLVATKDSMVTLAAALYPLQEANREKSKEREGAMYRLGPR
                     YAEALLAKNGGLVSPDANSTLRVTYGRVLGVPARDGLLYAPQTTLAGVAEKATGKGEF
                     NAPKAQLDAIKALRAGKASPYVDPKLNDVPVNFLSTVDTTGGNSGSATLNARGELCGL
                     LFDGTFDTVASDYLFDTARTRSIHVDSRYMLWTMAEVDGATNLLKEIGEAPSLDVKAA
                     H"
     gene            11001..12017
                     /gene="nadC"
                     /locus_tag="PLB01_05530"
     CDS             11001..12017
                     /gene="nadC"
                     /locus_tag="PLB01_05530"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQQ76795.1"
                     /translation="MTKLSRDLADDLDDLLSRAFAEDGEDVTSLAVFGPAARLSARIV
                     CRERAVVAGAAFLPRLFAFLSPPARVTVLVEDGTLVAPGTTLAEIEGPAITVLAAERT
                     ALNLLQRLSGVATKTREYVDALKGTKAVLLDTRKTTAGLRRLERYAVRCGGGTNHRYG
                     LAWGFLVKDNHADGAGSVWEATRRAKDFRALNRRLAPLLLEAEARTRDEVHQALDAGA
                     ERILLDNMSAAATADAAEDVHLYNEATGSRVTTEASGRMTPETARRAAEAGVDFVSAG
                     AITHSIRAIDIALDVDTTAPKNRKKKISLKVKGAGRPARHSRRTTRSSRTRIRRSPRS
                     SPPK"
     gene            12014..13612
                     /gene="nadB"
                     /locus_tag="PLB01_05535"
     CDS             12014..13612
                     /gene="nadB"
                     /locus_tag="PLB01_05535"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQQ76796.1"
                     /translation="MKSFPSRLHVPVLVLGSGIAGLSTAYRLARRGVKVLVLTKAADP
                     AECNTAWAQGGIIYFGKGDSQASLAKDILRAGAGLCRPEAVELLARRGPEVVKSILLR
                     DAGVPFSRTPRGDLDFTREGAHSIARIVHSADATGRAIETAMLARLKSEKNVTVWTEA
                     TAIDLITARHHSTDVAQRYALQDPCLGAYVLDAEARVHTVLADFTVLATGGVGRLFLH
                     TTNTRHAIGDGLAMAERAGAATLNLEYVQFHPTALYHEDAEGFLISESLRGEGAVLVN
                     RAGQAFMSRYDRRRDLAPRDIVTRAIVTEMTERGDPCVYLDLARHFKSHDGLGIRERF
                     PTIAARCAELGVDIASEPIPVVPAAHYHCGGVLVDLDGRTTIRRLYAVGEVSCTGVHG
                     ANRLASTSLLEGLVWGDAAATAIAARLKKEGRQSAGAFRAIPDWKSPGDAANEDPALL
                     QQDWATIRNTMWNYVGIVRTTARLARAVADLRDLEKRILRFYQVTKISKEIVDLFHGV
                     RAAHAIATAAFKNPVSKGCHYRKN"
     gene            complement(13609..14535)
                     /locus_tag="PLB01_05540"
     CDS             complement(13609..14535)
                     /locus_tag="PLB01_05540"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQQ76797.1"
                     /translation="MADGRPRRGILVYNPKAGGAARRIDVPGVVRRAAARGLALDAVP
                     TERPRHATELVAARLQDALDLVAVAGGDGTLGEAAEALLGGNVPIAILPAGTTNVVAR
                     EFGVADAGVAEAALFSSRTRPLTVFHAAGRACLVGAGVGFDARVMTYTVPVLKRLFGR
                     AGIGWTATLEWIKYEFPVIEIEGVDAEGRPFRREATFVVSANTARYGGDPILSPHADP
                     TDDLLDLVLFTSRSRKTLMLFYHHLSGGKAAHLLDEGVSRMAVRSFTARSKAGYELEV
                     QVDGDAAGKTPVTVGPAAGRVEILVPEAGPDL"
     gene            complement(14528..15547)
                     /gene="ruvB"
                     /locus_tag="PLB01_05545"
     CDS             complement(14528..15547)
                     /gene="ruvB"
                     /locus_tag="PLB01_05545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQQ76798.1"
                     /translation="MDERILSGVLQTEEADPEITLRPRALPEFIGQPKLKETLGVFLQ
                     AARQRQEPLDHVLLSGPPGLGKTTLAHIIAREMGAQIRVTSGPVLEKAGDLAALLTNL
                     APGDVLFVDEIHRMPPAVEEILYPAMEDGKLDLVIGSGPGARTVELRLPPFTLVGATT
                     RAGLLSQPFTSRFGITHRLDYYDADALGTIVTRSSEILGCPVDADAAREIAKRSRGTP
                     RIANRLLRRVRDYAEVAGETRISARAAETALDKLEVDHHGLDEVDRRILSTMIERFGG
                     GPVGLSALAHSLGEDKGTLEDLYEPFLLQSGFLQRTPKGRVASALAYRHLGLKPASGP
                     GTLFG"
     gene            complement(15587..16180)
                     /gene="ruvA"
                     /locus_tag="PLB01_05550"
     CDS             complement(15587..16180)
                     /gene="ruvA"
                     /locus_tag="PLB01_05550"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQQ76799.1"
                     /translation="MIARLTGTILDLKTDRIVLDVHGVGYELAIPLGTFSALPAAGEK
                     AVLHVHTHVREDALQLFGFATPQEKYVFERLLSVSGIGPKVALTVLSGLPLPELVGAI
                     ASQNARVLATIPGVGKKLAERLGLELKEKLAGFGPGGAPGAPGGKTSVVEDAIGALQN
                     LGYKALQAEQAVESAVRLVGTGDLNKILSAALKSLAR"
     gene            complement(16204..18150)
                     /locus_tag="PLB01_05555"
     CDS             complement(16204..18150)
                     /locus_tag="PLB01_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQQ76800.1"
                     /translation="MLKQLRENFKHLKWILWGVIAVFVLFVFVDWGMGNTPGAGTDTT
                     LAAKAGGIRVTTAEFQKEYAFAEERYRQAYGKNFSPELARAMNLPEQVLNGMIDRRLL
                     RGEAEKLGIKVTDEELTQHLLGIKDPQSGRPLFMKDGVFVGDAAYKRILAANRLSPES
                     FEADSRDALVLEKLNRFYTQSMVVGDDDVKAEYEGRNVKAKIAYVLAPPAAAAPVAVS
                     DQEAEAWFKSNPTPYMQPEKRKAKYLLVETSKIRPSISVTDADVQADYTANTEAYRKG
                     EEVHARHILYKVDGASDAAQKAKADAAVRKLKGGADFAALAKAESDDAGSKANGGDLG
                     SFPRGRMVKEFEEAAFGAKEKEIVGPVRSSFGYHVIQVLERTGERVQPFFEVAPGIRQ
                     RLQEDRAREEAKRLAVSLSEKVAKLGKPSDDDLRKLASAGVTFNETDFLARTDAPAGI
                     GFNPQFTEKLFGLKEGETAPAGVSTARGEAIVKCVEIRKPGLPAFAEIKARVLADIQK
                     KKQDEATLASVKQALDSAGSLEAFAKKANLKIETPEAFPKSGPVPGLGAPKAILDAVF
                     AAKAGETKGPVAVDARGAVVFRVESVTPFDAAAFEKEKAALREQLRGQKASRVLQALI
                     QRQRADLKVEFNKELLSRMSGART"
     gene            18334..19761
                     /locus_tag="PLB01_05560"
     CDS             18334..19761
                     /locus_tag="PLB01_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQQ76801.1"
                     /translation="MMDNKKGRDLESQLEWYYVSTQTLVRIVVGFLVIAGLVAGGVFF
                     FVKRDDSARRAAQEIADAADVLARAKKQQDAPLLQREISAADEKLLDARKDLAAGQNE
                     RATRTALDVKSTALKIMAGAVIKSEAHVADVAGTVQVQRANRTTWETLRQSMPLHEGD
                     FVKTGPNGTAEVLWNDGTMYRIRPETLFEVHANAGGSNPKLVVGTTDVSTGDKSRSKV
                     STDVATASIESNSSVGMQQDATSTSVSSYRGSTVLSNATGQSVTLGARERVVAARGGP
                     IGPKTSLPDAPALLAPDDNLLVDLRKTEPLRLRWSPVKDASGYQVEIAASRLFVKDSV
                     LPGFPSERPQPDTLIQVHDPGLYFWHVRTVKKGSPTLYSEWTAERRFKAVGGDQAAVQ
                     GDALPPDLVITQRPNVVGSSVVLVGRTVPGALVTVNGEMTDVNADGSFRKIVTIGGEG
                     MQTIVIKARTAGGETVKRETVLISN"
     gene            19802..>20537
                     /locus_tag="PLB01_05565"
     CDS             19802..>20537
                     /locus_tag="PLB01_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQQ76802.1"
                     /translation="MALRSFFSIFSSDLAIDLGTANTLVFAEGRGIVVREPSIVAVNK
                     ISNKVEAVGSAAKEMLGRTPGNIVAIRPMKDGVIADFEVTERMLDYFIKKAHGRSLFV
                     RPKIVISVPSDITQVEKRAVKDSALQAGASEVFIVEQAMAAAIGAGLPITEPTGNMIV
                     DIGGGTTDVAVISLAGIVYSKSVRVASNEMDEAIIQYIKRKYNLLIGERTAEQIKIEI
                     GSAYPLDEPISMEIKGRDLVEGVPKTL"
BASE COUNT         2989 a         7444 c         7052 g         3052 t
ORIGIN      
        1 ggtcgcgccg ggcacgctcg cctggaagcc cggcgccgcg ggctgggcgc cgatctcgag
       61 cttcgaggag ctcgcgggcc cgggttcggc ccaggctccg ccgcctcccc cgccgcccgc
      121 ccgctgacgc gccgccgacc atgaacgccg ccccgaccgt ggctcccgcc gcgccggcga
      181 agctccgcaa gtttccgtgt gccgcgtgcg gcgcggacgt cgtgtggagt cccggcgcgg
      241 cggccctgac gtgcccgtac tgcggcgcga agaaggaggt cccgaagact cccggtgagg
      301 tcgcggagcg cccgatcgag gaagcgctca agggcgcgac ggacctcggg tggggcctgg
      361 cgcgcaaggc cgtgagctgc cggggctgcg gcgcgaccac gacgttcgac gccggcgtcg
      421 cggcctcgcg ctgcgcgttc tgcggcgccc cgtccgtcgt cgaggcgccg ccctcgacga
      481 cgatggtccg tcccgcgggc gtcctgccgt tcaaggtcga tcgcaacgcg gccacgggtc
      541 gcttccggca gtggctgtcg tcgctgtggt tccggccgga cgacctccag cagaagtccg
      601 cgctctcgga gctgaagggc gtctacgtcc cgttctggac gttcgacgcg gccacgcact
      661 ccgcctggac cgccgacgcg ggctacgact accaggtcga ggtgcaggtc gaggagaacg
      721 ggcagtggca cacgcgctac gaaacccgga cgcgctggga gccggcgagc ggcgttctcg
      781 agcatttctt cgacgacctg cccgtcgccg cctcgcgcgg acttccgccg gacctcgcgc
      841 gcgccatcga gccgttcccc acggcggacc tcctcgcgta cgaaccgtcg tatctctcgg
      901 gtttcctggc ggaggagtac gcggtcggtc cgaaggaagc gctcgcttcc gggcagcagc
      961 gcatgacgca ggagctctac gccctctgcg ggcgcgaggt gccgggcgac cgcttccgga
     1021 acctccaggt gcggacggcc tggtccggcc tcgcgttcaa gagcgggctc ttcccggtct
     1081 ggatcgcggc ctaccagtac ggcgggaagc ccttccgctt cctcgtcaac ggcgtcacgg
     1141 gcaagaccga tggtcacgca ccgtggtcgt gggtcaagat cggcctcgcc gtcctcgcgg
     1201 caatcctcct cttcatcatc ctcgccgcga tcgcgggctg aggcggtccg cgccgccccg
     1261 tcagcgcccc gcggccttgc ggaacgcctc cgacttgagc cgacggacaa cctccgtcgt
     1321 ccacttgtag tcgacgctcg tgctgaagct cgcgtcaacg tagttcacgt tcttcgggtc
     1381 gaggtccccg agcgtgcctt tgaccacgaa cgagtcctcc cagaggatcc gcccgccgtc
     1441 gtccttgagc gagagccgcg tcgtcaccct gtagccgatc acgttgcccg ttttcgcgtt
     1501 cttctcataa acagagacgt accgaaccgc gacctccggg aaggagcctc gcttttccgt
     1561 caggcccgcc tgcttcaggg cgtcccgcga cttgatctca aacgtgacct tctggcggtc
     1621 gtcgacgttg aattccgacg cgaccgagag gccgacgggt cccaaggttc ccaacccttg
     1681 cgccccggcc ggggcgacgg ccgccggcgg cttcacgccg agcgccgcaa gggcgcccgg
     1741 ttccgccatc gcgcgcgcaa cctcgtccct ccatttgtcg agcgcccagc cccagacccg
     1801 attgaacatc tcggacggag agccgggctg ctcccccgtc gcgttgacct tcaggtcgcg
     1861 gctccagacg tcctggccgt ctcttctcgc ccggagcgtc ccccacaact cggccgtgta
     1921 cgaggaatag ccgtccatcc agaacttcga gatcgccacc tcgagcgtcg gtccggcgtc
     1981 ggccgtgccg acaccggcct tcgcgaggcc gctcttcgtg gccgcctcca cgaggcggcg
     2041 aaccgcgtcc ggcgactgct cgcgcatgcc ggcgctgttc ccggcgagag cgcggatcga
     2101 tccgacccgg ctcgggtcgt cgcccccttc cttcgggggc cgccggtcgc tgacgacgag
     2161 cgtcaccgcg ggggcgccgg ccggcggcgc gggcacgctc ccgggagtcg cgatcgccgt
     2221 cgtgtacatc cccgagcagc ccgtgagggc ggtgaggaga gatccgagcg cgagcgggaa
     2281 gagacgggcc atgggcttca aggggcacct ccgtcgttcc tggggtccaa gagataagcg
     2341 gagaaacggg cctccttccc tcacgaggcc gtgaagaact tctccgccga ccggtaaact
     2401 cgagcacgtg ggttcgatga ccgaggcgag accgcccgcg tcgaggctgg cccgggccga
     2461 ctcgcgcccg attctcgtga cggcgctcgt cgtgggctgg atcctcctct gcggtctcgg
     2521 ggccctgcgc gtcctcgtcg tcctcgggag ctcggccgca tcgtccgccc tcggaacctc
     2581 cgacccgctg gcgcgcgcgg cggccgccgc tccggatctc gtgtccgacg aaacacggca
     2641 ggttctcgcc cagcgcgagc agcgcgcgtc cctggatcgg gccctcgccg ttccgctcct
     2701 tctcgtttcg acgctcggca tcgtgggcgc cgtcgcgctg ctgcgccgca ggacgtggtc
     2761 ccgcgcgctc ctcctgaccg tcggcctgct cgccatcggc atctccgcct tccacgcgta
     2821 ccggtcggtc ggaatcgcgg ccgctccggc cgccggactc gaggcggagc cggacgccca
     2881 gaacgccctc gcccttcttc gggccgcggc cgggatcggt cttgcccttc agtcgattcc
     2941 gctggtcgtc gcgatgagcc tgctgaggca tcccatcgtc cgcgactacc tgggcgtgga
     3001 gacgcgcccg cgatcgggtc ccgatccgct cctcgtcgtc gccgcgtgcg cggtgctcgt
     3061 cgccgccggc gccgttctct tctcgaagag ccgcgccccg gcgccggcgc cggcgaagag
     3121 cgcgccggcc ttccatcccc ccgagcccgc agagacgttc cgctggagcg atcagccgat
     3181 cacgttctcg ccgcccgcgg gaccctggac gcgcgagcgc catgccgagg gcggccgcaa
     3241 gggcgtgtcg ttcacgcgct acgcgacgcc cccgagccgc atcatcgtcg cggaggcctt
     3301 cgtggaaatc ccgccgcgaa cggtcgacga ggtccttccc cggttccggc tgaccaaaga
     3361 gaagttcccc tccgtcgatt cgatcaccgt cggcgagccg gcgcccgcgg ccgtcgcggg
     3421 ccatgccgcc ttccagacgg actacacgat gcgcgaacgg tccatgcagc accgggggcg
     3481 cgagttcctg acggtcgtct cggggcacgt gttcgtcttc actctcctcg gcccggaagc
     3541 ggacatccgc accttcgaga acctcgtcgc gtccgttcga ttcacgggcg cggacgaggc
     3601 cggcggcgcc gcggcctctc cggcggcagc cgccgcggcc tctcccgacg agccgcccgg
     3661 cggcgacgtc accgtcgtcc gcgtcggcga acggcgggtg atcgtccgga tcccggcagg
     3721 ctgggagcgc gtcgactacg acaaacgcca ggagtttcag cgcggcgagg cgcgcttcgc
     3781 cctgttcgac gcgggtccgg cccagcccgg gcccgcgggc gaggtcgacg acccggacct
     3841 cgtcagacgc gccctcaaca ccatcggaca cgatccggcc cggcgggaag tcgcatcccg
     3901 gacgcccacc cagatcggcg gccgggacgc cctcgccgtc gacacgtggg aaccgctgag
     3961 ccacgcctac cgcgaacgct tctacttcgt cctgaacgcg gggaggctgc tggtcgcggc
     4021 ccccgtcatg ggccgccacg aggacacgaa ggctcccctc gaagcgctcg tccggtcgat
     4081 ccgtttcgtc gactagcccg ggcgccccgc gcgccggagc atccgctgcg gctccgtcct
     4141 cacgcggtcg ccgacggccc atgccgtgtc ggcgtcgaga gcctggatcg cgcgcagctc
     4201 ctcgccgtcg agcgtgcgct cgagcgccca cgtcgccccg ccgtcccgcg tgcgggtgac
     4261 gacggatcgg gcggcgttgt tcgggaggaa ccccacgatc cagccgtcgg cacccgcgaa
     4321 cgcgatgccc gtgaggaacg cgtcgcgggt gccggcgtcc acgagggtcc acgtgtcgcc
     4381 gccgtccgtc gtgcgaagaa cggttccgta ctccccgacg atccagccgt gtgacgcatc
     4441 gggccacgtg ccgccccaga ggtccggcgg gtgttccgtg ttcgccggtg cgggaatcgg
     4501 gacgcgggcc cacgtcgcgc cgccgtcgcg cgtgcgcagg agaacgccgt ccttgcctgc
     4561 gagccagccg ctccgcccgt ccgtgaagtg gaggacgcgc ccgttcgcgc ggcgcgcggg
     4621 ggagtctttc ccgtcgacca ccgtccaggt caggcccgcg tccgccgtgt ggatgacgcc
     4681 cgtcgcgccc gcggcccaac cctcgttccc gtcccggaag tcgagagcac tgaggaatcg
     4741 cgccccggga gccgtcgcgc gctgccagct cgcgccgccg tcgaacgtga ggaacacgcc
     4801 gtcgcccacg gcgcacgcgc gcatccggtc gaacccgtgg accgccccga gcccggacgt
     4861 ccgcccgccc ctcgtcacgc ccgtgacgta ccgccacgtc ctgccgccgt cctccgtccg
     4921 gccgacgatc ccgccgtcga cgaacgggct cccgccgacg atccagccga cgctcgggtc
     4981 gacgaagtga aggccgctga agacggcgtc cgttccgatc cggacttcct cccagccgcc
     5041 ggaggcgtcc cggacggcgg cgcgctccgt gctacgcccg caacgaacgg aagccatgag
     5101 gatcacgccc gcgaccgccg ctcggagaac ccacgcccgg gaacgtctct tcgctgtcat
     5161 ccggccaagg atagaacggg cttcagccgt tcagccgcca gatcgcaaac gtcagcgccg
     5221 tggcgaaggt gacccacgcg aggtagggcg cgaggagcca tgcagccggc ctcgagacac
     5281 ggccgaacgc gagcagcgtc gcgaggatcg cgagccacag cagcgcgatc tcgacgagcg
     5341 cggcgccgat cgcgtgccgt ccgaagaaga tccacgacca cgcgccgttc agggcgagct
     5401 ggacgaggaa gagccccagg gccgcacggc gcccgggggt cccggcgctc cgccacacga
     5461 gccatgccgc gatccccatg agcgcgtaga ggaggatcca tacgggcgcg aaaagtccgt
     5521 tcggcggatt ccacgcgggc ttcacgagcg ccgcgtgcca cgcccccggc ttccactgcg
     5581 acgcgatgaa gcccgtgccc tcgcagagga ggatccacag gacgagcccg agccactctc
     5641 ttgacctgtt cattcaccgc tcctgagccg gagtatctcg cacggactca tggactttcc
     5701 tagcgcgaga agaggtaggc gagggcgaag acgccgagca tcgcgaagac gatcgcgatc
     5761 ttgacggccg tgccgacggc gaggccgatc cacgtcgcga cgccggcctt gccggcctgc
     5821 cgccagtcgc gccggacggc gaactcgccg atcagggctc ccacgaacgg cccggcgagg
     5881 acgcccacga ggccgaggaa gagacccacg acggttccga tcgccgcgcc gacgagcgcg
     5941 cccttgctcg cgcccgccct cttcgcgccg agcgccgtcg agagaacgtc gaccgcgatg
     6001 gtggccgccg tgaggacgcc gagcacggcg agcgtccacg gcccgacgcg cgcgaacccg
     6061 tcgatccacg cggcgacgag gaggccggcg aagacgagcg gcgccccggg cagcgcagga
     6121 acgaccgtgc ccgcgaggcc ggcgagaacg agaagaaccg cgaggatcca gagtgcggcg
     6181 gtcaagacgg agcggaatgc taccgcttcg tcagcgtgac gaccgccgcg ccgtccgccg
     6241 gcgcgatccc gagcgtgatt cccttgctca tgaggttggc gccggtgtcc gtcgggagag
     6301 cctgaggcgc gcgcacggcc ggaaggctcg tgctcacggc cacgtcgtaa agcgcgctcg
     6361 ccgtgaggcc gaccgggaga acgcgcaggg ccgcattcgt gcccggagcc ggcgccgtgc
     6421 ccggcgtgct ccaaaccacg atcgtccccg cgcccgactt cgactcgtag ctctcgacgg
     6481 ccgcgatcgg gccctctccg ggctgggccg tcaggtggta gatcgcgccg tcgcggtgaa
     6541 cgacgcgctt ctgcttgaag agggcgatcg ccgctttcgc cgcctcctcg gtctcctggg
     6601 tccagtcggc gatccggccc atgaggagga acggcccgcc cgggagcgcc gacaggaagc
     6661 gatacgtgtc gggcgagccg tcgtcaccga cgtacttgtc gaggtagcgc ggcggcacga
     6721 ggtaggtgcc cccgtagatc gcgcgctggt tcccgtacgc gtcgttgcgg tcgctcgtgg
     6781 ccgacgtgtc gtggcgtgcg accatcccga agtcgaaaag ggccccgccg tcccagcagt
     6841 tctcgaggac gagcttcggg tgcctcttgc ggatctcgtc gagcacggcg tagtagccgg
     6901 cggtcgacgc ccaccagccg tcgccggcct ggtgggagtg gcccggcgcg tcgcagcccg
     6961 tgatgaccgt gaaatcgtgg acgagccagt cgagcgaccc ctcggtggcg aggcggtcca
     7021 cctgcgcgag gatccaggcc ctgacccgcg ggtccgcgag gcacagggcg acgccgtcca
     7081 tgtccgtggc gaccgtctcg ccgccgacgc gcgcgagcca gtccgggtgc tcgagcacca
     7141 cctgcgagag cgggtccgcc gcgccgagtg cgatccagag gccgaagcgc atccccttcc
     7201 cgtgggcgcg cgccgcgagg ttcttcagcc cgccggggaa cttcagggcg tccgctaccc
     7261 agtcgcccat gcgggggtac cagcccgcgt cgaccgtgaa gacttcgacg cccagccacg
     7321 ccgcccggtc gatgaggtcc tcgacctgcg gctcgacgat cccgtagctg tagccccacg
     7381 tgtcgaagcc ggcccacgga aagtccggcg ccggcagcgg cggggcgatg cggcgcgtca
     7441 cgaacgcgcg cgtcgcggcg ccggccgcgt cgagattccc ggagaacgtg ccgaggaacg
     7501 cggtcggcga ggagaacgtc tccgcaggcg cgagaacgtg accaaacgtc gcgggcgcga
     7561 atccgacgcc gaggctcgtg ctcccgtcgg ccgcggtggc ggccgcgagg cggccggggc
     7621 cgctccactc ccacccggcg aagacgccgg cgttccactt cgcggagcgg agcgcgagcc
     7681 acgccgcctg gtttccgaac gggccggtgc gcgcctgcac gacggatccg ggcttgagcg
     7741 tcgcctgcga ggtgtggaac ggcgtcgccg gatgccccca gttgaagttg ttcacggtca
     7801 gggacgtgag cgtgccgccg gccgcggtga gtccggcgtc gaggccgtcg acggcctcga
     7861 gaagaacggg ctgcgtcccg gtgttcgtca cggccaccga cgagcggaag acgggcgact
     7921 cggtgtagga aacgagagac cacgtgaccg agaccggcgc ctcgcgcgag gcgaggacga
     7981 ggtcgaagcg aacggccccg tccgacgaag gtttcgacgt cgcgcgcacg aggtcccacg
     8041 tcgacgcttc cgtgagcgtg acggggtcga agccggcggg gtcggctccg gccggcgcgt
     8101 cgggcgacgg gacgcgcagg acgacgcgga agccgaggcc gccttcggag gacgcccagt
     8161 ccagcccgcg cgaacggtcc ctcaggccca tcgtgacgat gcggccttcg ttcgtgtacc
     8221 tcaggctggt cgacagcagg gtgtttccga cggtccacgt gcggctgtcg gcatcctgct
     8281 ccccggaggt cgcggcagcc gcccgcccgg ccgcaagcgc ggtcacgagg acgaacaggg
     8341 cgcctcccgc cacttttttc aaccgcgtcg tcattcgatc gccactttct tgccgcccgc
     8401 gggccggagt tgagacgcag gcgcttcgaa gcgggattac gcaaggaaca gaccgaacgc
     8461 gagcccggac acaaatgtgt gcctcgtgac acacaatttc gtgacgcgag ttgcctctcg
     8521 ggagtgtcgg acgaaggacc gcgcctaacg cgcccggttt ccgccgcgcc gggaaaagaa
     8581 aaaggccgcc cgaaggcggc ctcgaaaatg gcattttggc ttccgaaggg tcagtgggcc
     8641 gctttcacgt cgaggctcgg agcctcgccg atttccttga gcaggttcgt cgcgccgtcc
     8701 acctcggcca tggtccagag catgtatcgg ctgtcgacgt ggatggaacg cgtccgcgcg
     8761 gtgtcgaaga ggtagtccga cgccaccgtg tcgaacgttc cgtcgaacag gaggccgcag
     8821 agctcgccgc gcgcgttcag cgtggccgag cccgagttgc cgcccgtcgt gtcgaccgtc
     8881 gagaggaagt tcacgggcac gtcgttcagc ttcgggtcca cgtacgggct cgccttgccc
     8941 gcgcgcagcg ccttgatcgc gtcgagctgg gccttcgggg cgttgaactc gcccttgccc
     9001 gtcgccttct cggcgacgcc cgcgagcgtc gtctgcggcg cgtagaggag tccgtcgcgc
     9061 gccgggacgc cgaggaccct gccgtacgtg acgcggagcg tggagttcgc gtcgggcgag
     9121 acgagtccgc cgttcttcgc gagaagggct tcggcgtagc gcggcccgag ccggtacatc
     9181 gcgccctcgc gctccttgct cttctcgcgg ttcgcctcct gcagcgggta gagcgcggcg
     9241 gcgagcgtca ccatcgagtc cttcgtcgcg acgaggtccg aggcggactt ctcgaagagc
     9301 gacatccgga agtccttgtc gtagatcttc gtgcccgcga tcgtcctgtc gagccacgcg
     9361 tcgatcgcct tgccggcgtc cgcctccggc atgccgggct tcaggccgat ctccttgtcg
     9421 aggacgtcga tccgctgttc cgccggcagc ttcgcgacgt cggcgagcgc gtacgccatg
     9481 agggcgcggt cggccttcgg ggagagcgtg cgctgggcgc gctcctgcga ctcgcgcagg
     9541 cgggtccagt tgcgctcctg gtatgccgga tcacggtctg cgtccttctt cgccttctcg
     9601 atcgaatacc ggtagaggat ctgcgaggcg ccgagcgccg agccgaccat catgccgccc
     9661 gatccgccga gttccgtgag gagcgcgttg cgctcgcgcg tcttcacgcg ctcggcggtc
     9721 agggcgttga tcgcggggat cacgtcgccg tacttcgcct tgcgggcggc gtcggcgtcg
     9781 atccacttca cgaagtcctt ctccatccgc tccttgtccg cgaggaggcc tcccttgacc
     9841 atgccctgaa ggacgccctt gttcttcgtg aggccgttgt tgaggcctcg gatcgcctgg
     9901 gcgacgcgca gcgccgtctc cttgtcgtcc ttgccgagct tctcgaggat cgcgatccgg
     9961 tcggtggagc gccggatcga gcgcgggagc gtccactcga ctgtctcctt gacgtcctcg
    10021 taggggttca ggcgcgccgt gcgccccggg tacccggcga cgaagacgag gtcgcccggg
    10081 ttcgcccccg cgggagacac cttgagccag tgcttcggct tgaagggaac gttgtccttc
    10141 gagaagggca cggccttccc gtccttcgag acgtaggcgc ggtagaagga ccagtcgccc
    10201 gtgtggcggg gccactgcca gttgtccgtc tcgccgccga aattgccgat gccggccggc
    10261 ggcgcgtaga cgagccgcac gtcctggatc tccatctgcg cgagctcgaa ccacttgagc
    10321 ccctcgaaga acggcgagac cgagcaacgc aggccgccct tctcgcactc ggccgtcttc
    10381 gccttgacgc gctgctcgat cgcgtcgaag cgcttgcggt ccgtgagctt cgcgtcgacc
    10441 ctgccggcga tctcatccgt gacctccttc acggagacgg tcacgtagac gcgcgagccg
    10501 ggcccgttcc agagctcgtc ggcgcgggtc ttcgcgaggt agccgtcgac catcaggttg
    10561 cggtcgggct tcgagttgta ctggagcgct ccctgcacgc agtggttgtt cgtgacgatg
    10621 agcccgtcgg gcgacacgaa cgaggcgctg cagccgccga gggagacgat ggcgcccatc
    10681 ggctggcccg tgaggtccgc aaaggcctgt gggtcgccct ggaagcccat ctccttcagg
    10741 cggggggcga gctgcgggat ttgctggggc atccacatgc cttcgtcggc cagggcgccg
    10801 gcggcgagga gcacgaggac gctcacgagg acgaggcgtc cggaacgtac gttcacgagt
    10861 ttctccttcg gaatcgacgt ggcgcgcccg ccgggcgcgc aaagcgctcg attatgcccc
    10921 gcagaacggg cgccgacatc cctatcattt gcatcggtgc tgcccgccgc ccggcccggc
    10981 ctcaagtatt ctgtgcgggc gtgacgaagc tctcccggga cctcgcggac gacctcgacg
    11041 acctcctctc ccgcgctttt gccgaggacg gcgaggacgt gacttcgctc gccgtcttcg
    11101 ggccggccgc ccggctctcg gcccggatcg tctgccggga gcgcgccgtc gtcgccggag
    11161 cggcgttcct cccccgcctc ttcgcctttc tctcgccgcc agcgcgcgtg accgtcctcg
    11221 tcgaggacgg gacgctcgtc gcccccggga cgaccctcgc cgagatcgag gggccggcga
    11281 tcacggtcct cgcggccgaa cgcaccgcgc tcaacctcct ccagcgcctg agcggcgtgg
    11341 ccacgaagac ccgcgaatac gtggacgcgc tcaaggggac gaaggccgtc ctcctcgaca
    11401 cgaggaagac gaccgcaggg ctgcgccggc tcgagcggta cgccgtgcgg tgcggcggcg
    11461 gcacgaacca ccgctacggc ctcgcgtggg gcttcctcgt gaaggacaac cacgccgacg
    11521 gcgcgggaag cgtgtgggag gcgacgcgcc gcgcgaagga cttccgcgcg ctcaaccggc
    11581 ggctcgcgcc gctgctcctc gaggccgagg cccgcacgcg cgacgaggtg caccaggccc
    11641 tcgacgccgg cgccgagcgc atcctgctcg acaacatgtc ggccgccgcg acggctgacg
    11701 ccgccgagga tgtccacctc tacaacgagg cgacgggctc ccgggtcacg acggaagcgt
    11761 ccgggcgcat gacgcccgag acggcgcgga gggccgccga ggccggcgtc gacttcgtct
    11821 ccgcgggcgc catcacgcac tccatccgcg cgatcgacat cgccctcgac gtcgacacga
    11881 ccgccccgaa gaacaggaag aagaagattt ctctgaaggt gaaaggggca gggcgaccgg
    11941 cgcggcacag ccggcggacc acgcgcagtt cccgcacgcg cattcgaaga agtccccgtt
    12001 cttctccccc taaatgaagt ccttcccctc ccggctccac gtccccgtcc tcgtcctcgg
    12061 gtcgggaatt gcgggtctct ccacggccta ccgcctcgcg cgccgcggcg tgaaggtcct
    12121 cgtcctcacg aaggccgcgg atccggccga gtgcaacacg gcgtgggcgc agggcgggat
    12181 catctacttc ggcaagggcg actcgcaggc ctctctcgcg aaggacatcc tccgcgcggg
    12241 cgccggcctc tgccggcccg aggccgtcga gctcctcgcg cggcgcgggc ccgaggtcgt
    12301 gaagagcatc ctcctccgcg acgccggcgt gccgttctcg cgcacgcccc ggggcgacct
    12361 cgacttcacg cgcgagggcg cccactcgat cgcccggatc gtccattcgg ccgacgcgac
    12421 cggaagggcg atcgagaccg cgatgctcgc gcgcctgaag agcgagaaga acgtcaccgt
    12481 ctggaccgag gccacggcga tcgacctcat cacggcccgc caccactcga cggacgtcgc
    12541 gcagcgctac gcgctccagg acccctgcct cggcgcctac gtcctcgacg cggaagcccg
    12601 ggtccacacg gtcctcgcgg acttcacggt cctcgcgacc ggcggcgtcg ggcggctctt
    12661 cctccacacg acgaacacac ggcacgccat cggcgacggc ctcgcgatgg ccgagcgcgc
    12721 gggcgccgcg acgctgaacc tcgagtacgt ccagttccac ccgacggcgc tctaccacga
    12781 ggacgccgag ggcttcctga tctccgagtc gctgcgcggc gagggcgccg tcctcgtgaa
    12841 tcgcgcgggc caggcgttca tgtcgcgcta cgaccgccgc agggacctcg cgccccgcga
    12901 catcgtgacg cgcgcgatcg tcacggagat gacggagcgc ggggacccct gcgtctacct
    12961 cgatctcgcg cggcacttca agtcgcacga cgggctcgga atccgcgagc gcttcccgac
    13021 gatcgccgcg cgctgcgcgg agctcggtgt cgacatcgcg agcgagccga tcccggtcgt
    13081 tccggccgcg cattaccact gcggcggtgt cctcgtggac ctcgacgggc gcacgacgat
    13141 ccgccgcctc tacgccgtcg gcgaggtgag ctgcacgggc gtccacgggg cgaaccggct
    13201 cgcctcgacg tcgctcctcg aggggctcgt ctggggcgac gcggccgcga cggcgatcgc
    13261 ggcgcgcctg aagaaggaag ggcgccagag cgcgggcgcg ttccgcgcga tcccggactg
    13321 gaagtcgccc ggcgacgcgg cgaacgagga cccggcgctc ctccagcagg actgggcgac
    13381 gatccggaac acgatgtgga actacgtggg gatcgtgcgg acgaccgcgc gcctcgcgcg
    13441 cgccgtcgcg gacctgcgcg atctcgagaa gcggatcctg cgcttctacc aggtcacgaa
    13501 gatcagcaag gagatcgtcg acctcttcca cggcgtccgc gccgcccacg cgatcgcgac
    13561 ggcggctttc aagaaccccg tctcgaaggg ctgccactac cggaagaact agaggtccgg
    13621 gccggcctcc gggaccagga tctcgacgcg gccggccgcc gggccgaccg tgacgggcgt
    13681 cttgccggcc gcgtcgccgt ccacctgcac ctcgagctcg tagcccgctt tcgagcgcgc
    13741 cgtgaacgag cgcacggcca tccgcgagac gccctcgtcc aaaaggtgcg cggccttccc
    13801 accggagagg tggtggtaga agagcatcag cgtcttgcgc gagcgcgagg tgaagagaac
    13861 gaggtcgagg agatcgtccg tcgggtccgc atggggcgag aggatcggat cgccgccgta
    13921 gcgtgccgtg ttcgccgaga cgacgaacgt cgcctcgcgg cggaacggcc tcccctccgc
    13981 gtccacgccc tcgatctcga tcacggggaa ctcgtacttg atccactcca gcgtcgccgt
    14041 ccagccgatg ccggcgcggc cgaagagcct cttgaggacc gggacggtgt acgtcatgac
    14101 gcgcgcatcg aacccgacgc ccgcgccgac gaggcaggcg cgtcccgccg cgtggaacac
    14161 cgtgaggggg cgcgtccggg acgagaagag cgccgcctcc gccacgccgg cgtccgcgac
    14221 gccgaactcg cgcgcgacga cgttcgtcgt cccggcgggc aggatcgcga tcgggacgtt
    14281 ccccccgagc agggcctcgg ccgcctcgcc gagagtcccg tcgccgcccg cgaccgccac
    14341 gaggtcgaga gcgtcctgga gccgcgcggc gacgagctcg gtcgcatggc gcggccgctc
    14401 cgtcgggacc gcgtcgagcg cgaggccgcg tgcggccgcg cgtctcacga cgccgggcac
    14461 gtcgatccgc cgcgcggcgc cgcccgcctt cgggttgtaa acgaggatcc cgcgccgcgg
    14521 gcgtccgtca gccaaacagc gttcccggac ccgaggccgg cttgaggccg aggtgccggt
    14581 aggcgagcgc cgacgcgacg cgccccttcg gggtgcgctg gagaaacccc gactgcagga
    14641 ggaacggctc gtagaggtcc tcgagcgtgc ccttgtcctc tccgagcgaa tgtgcgagcg
    14701 ccgacaggcc caccggaccg cctccgaacc gctcgatcat cgtcgagagg atccggcggt
    14761 cgacctcgtc gaggccgtgg tggtccacct cgagcttgtc gagcgcggtc tcggcggcgc
    14821 gcgcggagat gcgcgtctcc cccgcgacct cggcgtagtc gcgcacgcgg cgcaggaggc
    14881 ggttcgcgat acgcggcgtg ccgcggctgc gtttggcgat ctcgcgcgcg gcgtcggcgt
    14941 ccaccgggca tccgaggatc tcggaggagc gcgtcacgat cgtccccagg gcgtcggcgt
    15001 cgtagtagtc gaggcgatgc gtgatcccga agcgggaggt gaacggctgc gagaggagcc
    15061 ctgcgcgcgt cgtcgccccg acgagcgtga acggggggag cctcagctcc accgtccgcg
    15121 cgccggggcc cgagccgatc acgaggtcga gttttccgtc ctccatcgcg gggtagagga
    15181 tctcctcgac ggcgggcggc atccggtgga tctcatccac gaacaggacg tcgccgggcg
    15241 cgaggttcgt caggagcgcc gcgaggtcgc ccgccttctc gaggacaggg ccggacgtca
    15301 cgcggatctg cgcccccatc tcgcgggcga tgatgtgcgc gagcgtcgtc ttgccgaggc
    15361 ccggggggcc cgacaggagg acgtggtcga gcggctcctg ccgctgccgc gccgcctgca
    15421 ggaagacgcc gagcgtctcc ttcagcttcg gctgcccgat gaactccggc agcgcgcgcg
    15481 gcctgagcgt gatctccgga tcggcctcct ccgtctggag gacgcccgag agaatccgtt
    15541 cgtccatgcg ccgattatgg cgcgacgagg cgggccgagg agctcctcat cgggccagcg
    15601 acttcagcgc ggccgacagg atcttgttga ggtcgcccgt cccgacgagg cgcaccgcgc
    15661 tttcgacggc ctgttcggcc tggagggcct tgtagccgag gttctggagc gcgccgatcg
    15721 cgtcctccac gacggacgtc ttgccgcccg gcgcgccggg cgcgccgccg gggccgaacc
    15781 ccgcgagttt ctccttcagc tcgaggccga ggcgttcggc cagcttcttg ccgacccccg
    15841 ggatcgtcgc gaggacccgc gcgttctgcg aggcgatcgc cccgacgagc tccggcagcg
    15901 gcagacccga gagcaccgtg agggcgacct tcggcccgat gcccgagacg gaaaggagcc
    15961 gctcgaagac gtacttttcc tgcggcgtcg cgaacccgaa gagctggagt gcgtcctcgc
    16021 ggacgtgcgt gtggacgtgg agcacggcct tctcgccggc ggccggcagc gccgagaagg
    16081 tcccgagggg gatcgcgagc tcgtagccga cgccgtggac gtcgaggacg atccggtccg
    16141 tcttgaggtc gaggatcgtc cccgtcaggc gcgcgatcac gtccctatgt tacgtgcccc
    16201 gctctacgtg cgcgctccgg acattctcga gagaagctcc ttgttgaact ccaccttgag
    16261 gtcggcccgc tgccgctgga tgagggcctg gagaacgcgg gaggccttct ggccgcgcag
    16321 ctgctctctc agggcggcct tttccttctc gaacgccgcc gcgtcgaacg gcgtgacgct
    16381 ctcgacgcgg aacacgacgg cgccgcgcgc gtccaccgcg accggcccct tcgtctcgcc
    16441 cgccttcgcg gcgaacacgg cgtcgaggat ggccttcggc gcgccgaggc ccgggacggg
    16501 cccggacttc gggaacgcct cgggcgtctc gatcttgagg ttcgccttct tcgcgaacgc
    16561 ctcgagcgac ccggcggagt cgagggcctg cttcacgctc gcgagcgtcg cctcgtcctg
    16621 cttcttcttc tggatgtcgg cgaggacgcg cgccttgatc tcggcgaacg ccgggagccc
    16681 gggcttccgg atctcgacgc acttcacgat cgcctcgccg cgtgccgtcg agacgccggc
    16741 gggcgccgtc tcaccctcct tgaggccgaa gagcttctcc gtgaactgag ggttgaaccc
    16801 gatccccgcg ggcgcatccg tccgcgcgag gaagtccgtc tcgttgaacg tgacgccggc
    16861 ggaggcgagc ttccggaggt cgtcgtccga gggcttcccg agcttcgcga ctttctcgga
    16921 cagcgagacc gcgagcctct tggcttcctc gcgcgcgcgg tcctcctgga gacgctggcg
    16981 aatgccgggc gcgacctcga agaacggctg gacgcgctct ccggtccgct cgagaacctg
    17041 gatcacgtgg tacccgaagc tcgaccggac cggcccgacg atctccttct ccttcgcgcc
    17101 gaacgcggcc tcctcgaact ccttgaccat ccgtcccctc gggaaggacc cgaggtcgcc
    17161 gccgttggcc ttcgagccgg cgtcgtcgga ctcggccttc gcgagcgcgg cgaagtccgc
    17221 gccccccttc agcttccgga cggcggcgtc ggccttcgcc ttctgggcgg cgtcgctggc
    17281 gccgtcgacc ttgtagagga tgtgacgcgc gtggacctct tcgcccttgc ggtaggcctc
    17341 ggtgttcgcg gtgtagtcgg cctgcacgtc ggcgtccgtc accgaaatcg aggggcggat
    17401 cttcgaggtc tcgacgagaa ggtacttcgc cttgcgtttc tcgggctgca tgtagggcgt
    17461 cgggttcgac ttgaaccacg cctcggcttc ctggtcggag acggccacgg gcgccgcggc
    17521 ggccggcggc gcgagcacgt aagcaatctt cgccttcacg ttgcggcctt cgtactcggc
    17581 cttcacgtcg tcgtcgccga cgaccatcga ctgcgtgtag aaccggttga gtttctcgag
    17641 gacgagcgcg tcgcgcgagt cggcctcgaa gctctcgggc gagaggcggt tcgcggcgag
    17701 gatccgcttg tacgcggcgt cgcccacgaa aacgccgtcc ttcatgaaca gcggccggcc
    17761 cgactgcggg tcctttatgc cgaggaggtg ctgcgtgagc tcctcgtccg tcaccttgat
    17821 tcccagtttt tccgcctcgc cgcgcaggag ccgccggtcg atcatcccgt tcaggacctg
    17881 ctcgggcagg ttcatcgcgc gcgcgagctc gggactgaag ttcttgccgt aggcctggcg
    17941 gtagcgctcc tcggcgaagg cgtattcctt ctggaactcc gccgtcgtca cgcggatgcc
    18001 gcccgccttc gcggcgagcg tcgtgtcggt cccggcgccg ggcgtgttgc ccattcccca
    18061 gtcgacgaag acgaacagga cgaagacggc gatgaccccc cagaggatcc acttgaggtg
    18121 cttgaagttc tcgcgcagct gtttgagcat ttcttctccg gaaccgggcg cggcccgcat
    18181 cgcgcggtgc cgccacccga aagaacgggc gacgatacct taccgacagg cggcggtaaa
    18241 gttggcggct gcggcccggc ccggtgcacc cggcaaggcc ttcgcgtgct ataacatccg
    18301 tttctagaag ggtttccgga aaacgaaggc gccatgatgg acaacaagaa gggtcgagac
    18361 ctcgagtcgc agctcgagtg gtactacgtc tcgacgcaga cgctcgtccg gatcgtcgtg
    18421 gggttcctcg tgatcgcggg cctcgtcgcg ggcggtgtct tcttcttcgt gaagcgggac
    18481 gactccgcgc gccgcgccgc gcaggagatc gccgacgccg ccgacgtcct cgcacgcgcc
    18541 aagaaacagc aggacgctcc gctcctccag cgcgagatct ccgcggccga cgagaagctc
    18601 ctcgacgcgc gcaaggacct cgcggccggg cagaacgagc gcgcgacgag gacggcgctc
    18661 gacgtgaagt ccaccgcgct gaagatcatg gcgggcgccg tcatcaagag cgaggcgcac
    18721 gtcgcggacg tcgcgggcac cgtgcaggtc cagcgcgcga accgcacgac ctgggagacg
    18781 ctccgccaga gcatgcctct ccacgagggc gacttcgtca agaccggccc gaacggcacg
    18841 gccgaggtcc tctggaacga cgggacgatg taccgcatcc ggcccgagac cctgttcgag
    18901 gtgcacgcga acgcgggcgg ctcgaacccg aagctcgtcg tcggcacgac cgacgtctcg
    18961 accggcgaca agagccgctc gaaggtgtcg accgacgtcg cgacggcgag catcgagtcg
    19021 aactcgtccg tcgggatgca gcaggacgcg acctccacgt ccgtgtcgtc ctaccgcggt
    19081 tcgaccgtcc tctcgaacgc cacgggccag agcgtcacgc tcggcgctcg cgagcgggtc
    19141 gtcgccgcac gcggcggccc catcggcccc aagacgtccc ttccggatgc gcccgccctc
    19201 ctggcgccgg acgacaacct cctcgtcgac ctccgcaaga ccgagcccct gcgcctccgt
    19261 tggtccccgg tcaaggacgc gagcggctac caggtggaga tcgccgcgag ccggctcttc
    19321 gtgaaggatt ccgtccttcc cggcttcccg tccgagcgcc cccagccgga cacgctcatc
    19381 caggtgcacg acccgggcct ctacttctgg cacgtgcgca ccgtgaagaa gggctccccg
    19441 acgctctact cggagtggac ggccgagcga cgcttcaagg ccgtcggcgg cgaccaggcg
    19501 gcggtccagg gggacgcgct cccgcccgac ctcgtgatca cgcagcgccc gaacgtcgtc
    19561 ggttcctccg tcgtcctcgt gggacggacg gtccccggag cacttgtcac cgtgaacggc
    19621 gagatgaccg acgtgaatgc cgacggaagc ttccggaaga tcgtcacgat cggcggcgag
    19681 ggcatgcaga cgatcgtgat caaggcccgc accgcgggcg gcgagacggt caaacgagaa
    19741 acggtcctca tcagcaactg accacggcgg ccttccgccg acccccgaaa ggaatcgtcc
    19801 cttggccctg cgctctttct tctcgatctt ctcgtccgac ctcgcgatcg acctcggcac
    19861 ggcgaacacg ctcgtgttcg cggaaggtcg cggcatcgtc gtccgcgaac cctccatcgt
    19921 ggccgtcaac aagatctcca acaaggtcga ggccgtcggg agcgccgcca aggagatgct
    19981 cggccgcacg cccggaaaca tcgtcgcgat ccggcccatg aaggacggcg tcatcgccga
    20041 cttcgaggtc acggaacgga tgctcgacta cttcatcaag aaggcgcacg gccgctcgct
    20101 cttcgtgcgc cccaagatcg tcatctccgt cccgtccgac atcacgcagg tcgagaagcg
    20161 cgccgtgaag gactccgccc tgcaggcggg cgcgagcgaa gtcttcatcg tggagcaggc
    20221 gatggcggcc gccatcggcg cgggcctccc gatcaccgag ccgacgggca acatgatcgt
    20281 cgacatcggg ggcggcacga cggacgtcgc cgtcatctcg ctcgcgggaa tcgtctactc
    20341 gaaatccgtg cgcgtcgcgt cgaacgaaat ggacgaggcg atcatccagt acatcaagag
    20401 gaagtacaac ctcctcatcg gcgagcgcac ggccgagcag atcaagatcg agatcggctc
    20461 cgcctacccg ctcgacgagc cgatctcgat ggagatcaag ggccgcgacc tcgtcgaggg
    20521 cgtgccgaag acgctca
//
LOCUS       DAOMFY010000042        19903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_861_length_19903_cov_7.418128, whole genome shotgun sequence.
ACCESSION   DAOMFY010000042 DAOMFY010000000
VERSION     DAOMFY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19903
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_861_length_19903_cov_7.418128"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2364)
                     /locus_tag="PLB01_05570"
     CDS             complement(<1..2364)
                     /locus_tag="PLB01_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HQQ76803.1"
                     /translation="MPAFREHDKLFHKFNPDLGPGRVEQVDGRRIVVWFPAADTRLTL
                     AADDNALRPAPLREGQKARLDGEEEPVVVDEVGAETSRLADGRVVTSARLWPLAAPDD
                     PIDRLGALQTDRAAAFRNRVDGLLLSEIRQARGLGSFLGGRIAIFPHQLHVAERAAAS
                     DPVRWLLADEVGLGKTIEACLVLSRLLRTDRADRALVVAPSTLTVQWLGELYRKFHQT
                     FVLLDRRRREDVAREHGEGFNPFEAHRHAIVALEDLAGETGLAAKAEAAGFDLLVVDE
                     AHRLERARGEAGSDAYLAVAPLTRAARHVLLLSATPLEADAHGFFRLLELLRPDAYTS
                     EEAFLASLEKDEPLPPCTSATRRVDIGGLMPRVPRPVDLGRVPAAEGLTGDDAADADD
                     PRVAWIAGEDAAWRADGSAKTLVFVHERATLSALKARLESATRKRVAIFHEELTPERG
                     DVEVAEFRRADGPSILVSTEAGGEGRNFEFCRRLVLFDLPADPAAIEQRIGRLDRINR
                     TSPVEIVYFRPPDGFGRELATLYERIGLFTSPLGGLERSLAGVEAGITRARRLGKPLP
                     VDDLAAEVARAAEARQKAVYHHLHRDGYAPEKAAGILARVPADLDARTERFVLEAADL
                     FGFETAERSGRRTWYLEFGADALVEHLPGVSGGSRWLGTFDRDEAVEKEDLDFFASGH
                     ALVEGVFRELEDGPRGRVALLLLEKTGVSGAGLLFALRRGASPEFVAASLDGREQPAW
                     AEILVARRAETRGIRPEDWTALLTRAARSGRAPDWAGLVRRAAKKVLA"
     gene            complement(2491..2994)
                     /locus_tag="PLB01_05575"
     CDS             complement(2491..2994)
                     /locus_tag="PLB01_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HQQ76804.1"
                     /translation="MEKRRTVLKLGFAAAASALLAPLRALVGSPTTSSPARLPQSIHP
                     TPAEEKVVRAFYGQGWSEGNTAVIASHPATAAECQRLLQRFRQAFPDLRVDVASITKV
                     GDETHVKWTAHGTNRGSLGGLAPTGRKVNIQGSTKMKIVDGKIVASTADWDESDLKRQ
                     LSTASAR"
     gene            3125..3958
                     /locus_tag="PLB01_05580"
     CDS             3125..3958
                     /locus_tag="PLB01_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HQQ76805.1"
                     /translation="MDLEPHPFNTGFAWSEVKGPFRRLSAAEAQSFNERGYVVLEDVF
                     DAGVLRALVDVIDPVEARVEAMLRGQPGQRIFVARAGEITFTVHLASLSPLVRAFCSG
                     PVFRELAHDLVGPDVRLYWDQAVYKKPSISGDLPWHQDNGYTFVEPQQTLTCWIALTD
                     TTEENGCLWLAPGLHRFGTLRHWRTEQGWCCLKEPPETAAAPLRAGSMAVFSGVTPHR
                     TGPNLTDRVRKAFILQFAKDGACTVAPDPGTGEPVRRPCNDPRCQFPVLAGGEAPSLS
                     R"
     gene            complement(3944..5908)
                     /locus_tag="PLB01_05585"
     CDS             complement(3944..5908)
                     /locus_tag="PLB01_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQQ76806.1"
                     /translation="MRAGFATLVDVLAAHAVERPGKTAVTFLEDGETRSVSWTYSDLY
                     GRAAGIASALRRRDLAGRTVLLAHPPGLDFIAALFGCFNAGAIAVPTCPPRSGRGRAD
                     IRLAAVVEDARPALALTDAAHLGSCRHETADTHVAPSLEWLATDDSRFTLRVERADLG
                     AEPDAIAMLQYTSGSTRAPAGVALSHRNLMENQHAIAEAMGHDENSRFVSWLPPYHDM
                     GLIGAILQPIALGSDVTLMAPKHAVERPIRWLRAISRTRATTSGAPDSMFAHCVRMVL
                     PEEREGLDLGSWRIAFNGSEPVRAETMQRFAAFFAPCGFRADAFFPCYGLAESTLLVS
                     GESGVRVEAFEPGARRFVSCGTPARGIEVAVVDPGSLRTCGEDETGEIWLSGTSVARG
                     YWKRPEATHAAFENGLPGRPGLFLRTGDLGLIRDGRLFVAGRLKDLVILGGRNYYPQD
                     IEACAGGSHAALSPTRCAAFSVGGLEGERLVVACEMPREAAPGLDTAVLLCSIRDEVA
                     SEMDVAVSAVQILKQGELPRTTSGKIRRGACRQAFLDRTWDVVDEWPSPVATPAALSP
                     TILDDLAREVTSVLNAPGRSLDPERPLAGQGLDSLGRVELILRLEAKFRVSFDPSSVS
                     PDATLRDLEAAVARARSGRSEVSDGPLSGE"
     gene            complement(5924..7042)
                     /locus_tag="PLB01_05590"
     CDS             complement(5924..7042)
                     /locus_tag="PLB01_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ76807.1"
                     /translation="MRFETLAVHAGRRPEAGTGSVAPPIYLSTTFERGETGVPLGGHT
                     YIRESNPMQTLLEDALAPLEGGEGALVLGSGMAAGVALFQALPRGAHVLLPDDAYYGW
                     RAVGQEFFPAWGLTASSCAMDDLAAVAKAIRPETKLVLVESPSNPLMKVADLAAIAAL
                     AKRAGALTLVDNTFATPALQRPLDLGADVALHSATKYFGGHSDVQGGALVFRRKDDVF
                     AKVAHVRHLLGAVLAPFNAWLVLRGLRSLAVRMNAHSANALAVARALEAHPRVSRVNY
                     PGLASHPGHAVAAKQMSGFGGMLSFHVKGGRDAAVAAVGRAKLFVRATSLGCVESLLE
                     HRATSEGPGSTTPQDLLRASIGLEHPDDLIADLDQALG"
     gene            7080..7805
                     /locus_tag="PLB01_05595"
     CDS             7080..7805
                     /locus_tag="PLB01_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76808.1"
                     /translation="MRLRTSAFLLATLIALPLAAQDPTPSKLDVGPGLKRERERLSNY
                     QWRLKTEMRVDGTLRMLKVEDVHLGPDGGLVKKTIKFDKPAEPTPFAENDPRARVYAP
                     TTGTEEERLSEIAQDLMQYYARLSPERVAEWAKNAEVLPPEADHPGLVRMHGRGLGRP
                     LDDAVLFFDPKTKTAAQIEVKTTVDPRILDIAFIRATFEPLPQVRPDVAPIWVPKRVF
                     LNMRRGKRAVALEMETTDWRQWP"
     gene            complement(7851..9071)
                     /locus_tag="PLB01_05600"
     CDS             complement(7851..9071)
                     /locus_tag="PLB01_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ76809.1"
                     /translation="MRDRLTRVFKAFTYRDFSLLWAGAFTSSVGTWMQSVAQSWLVLT
                     ISGNPQYLAWTEGLQQAPFLVFSLVGGVLADRMDRRRILLLSQVVQLTTAFLLAFLVY
                     ANHVQVWMILVLSLVVGIAQSFGGPAYQALVPTLVDKEDLPNAVALNSIQFNLARVIG
                     PVLAGAAFATLGAAACFGLNGLSFFAVILALLALRRGAPTGAASGSVMENLKAGLAGA
                     HRDRALRGLIALSFIGSFCAFPLLTFLPVFAKDVFKGDVKVLATFLAVFGLGAVSGAI
                     GAAGFGHVKRRGAVAVAMQMAFGALMIGFALSRTKWLSFPLLFLAGAALMIVFAMFMT
                     LVQTNVDDAMRGRVVSVYSLAFRGAMPLGNLAAGFLVGVMPAPWVLAGNGALLLLVGS
                     TVFLRNHPEGVPKL"
     gene            complement(9104..9889)
                     /gene="tatC"
                     /locus_tag="PLB01_05605"
     CDS             complement(9104..9889)
                     /gene="tatC"
                     /locus_tag="PLB01_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQQ76810.1"
                     /translation="MTDASRRPGGDPEEDLPRMSFLEHLEDLRKRLFWSAFAVAAGFM
                     VCWWKAKPIFNWLAKPVLAALPPGTKLAYTQLTEPFMLYINIALLAGVFVASPVILYQ
                     LWLFIAPGLYKHERRWVWPFVVLSVLFFVGGGYFGYAVGFPMVAQFLVATGEDFMPVI
                     KIDDYLDITSKILLGMGLVFETPILIFFLARMGVVTEKWLLAKFRYAVLVIFIIAALI
                     TPTPDIPTQCAFALPMCGLYLLGVAMAWVFKKRVPKDAAPTEN"
     gene            complement(9886..10179)
                     /locus_tag="PLB01_05610"
     CDS             complement(9886..10179)
                     /locus_tag="PLB01_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014514826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatA/E family twin arginine-targeting protein
                     translocase"
                     /protein_id="HQQ76811.1"
                     /translation="MGNLGMPELIMIMVLALLLFGPKKLPEIGKQVGKALGEFKRASN
                     DLKRTIEDEMEKASSAVKIDDPPKTEPENSAPALAPSDGTISRPSETTKTPAP"
     gene            10288..11958
                     /locus_tag="PLB01_05615"
     CDS             10288..11958
                     /locus_tag="PLB01_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQQ76812.1"
                     /translation="MNNSPVSPRSRSIRHALAAAVFLLAVTVGGGIAGGRLGAVSDAT
                     NPGFKEYSDILATARAWYADEVPANKLVYASIHGMLGTLDPHTNFLEPEEYSSMVEKQ
                     RGSFYGLGIIISKRNGKVTVITPVEGSPADRLGIRAGDVIDKVEGQPIDDLPVDAVVK
                     KLKGPKGTKVRITILRPGLDEPLQMTVTRAEIPTNSVSYAFLVEPGVGYLRLKDFTHT
                     SAPELAENWDKLEKQGMKKLVLDLRGNPGGLLDQAIAVSDFFLSRNEKVVVTRGRNGA
                     QEQSFAAPGKNTHARIPVVVLVNKGSASASEIVAGALQDHDRAVIVGQTTWGKGLVQS
                     VYNLSDGAGLALTSAKYYTPSGRCIQRDYKDVLEYLSHEDEEGGDAEETGPARDAASS
                     QKKESFKTDAGRTVYGGGGITPDVFVKAPTLSRFATTLYARGLFFEFAVDYLAKHSTV
                     PRDFPVTEAVRDDFFKFVDAKPGILLEKSAKATYAEEKDPLVVDRAIREEVLTAKFGR
                     DAGYRASLENDVQLKKALTLFPEAEKLATAHEQMQLAKKSELKGPLKP"
     gene            complement(11864..12472)
                     /locus_tag="PLB01_05620"
     CDS             complement(11864..12472)
                     /locus_tag="PLB01_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76813.1"
                     /translation="MLKLALRAALGFALGLGLWRAGTPAYNRLLAGFAEPVVRLLERP
                     AATRLYAEEARVVIDRADFTSRSDRPSLPADDLTFDVIILFTLAAATPGLFRDRAMRG
                     LGISLVVLFALHVAALVAAVQSFYALKLGAWSAAAYGPFARNFWATASHFWRLAGCFA
                     APFVLWWLLIRPTASGDPSTRTSSRAASARARSRASRPRGRG"
     gene            complement(12453..12962)
                     /gene="xrtH"
                     /locus_tag="PLB01_05625"
                     /pseudo
     CDS             complement(12453..12962)
                     /gene="xrtH"
                     /locus_tag="PLB01_05625"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
     gene            complement(13060..14748)
                     /locus_tag="PLB01_05630"
     CDS             complement(13060..14748)
                     /locus_tag="PLB01_05630"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ76814.1"
                     /translation="MIRKALILSATVAAVALLMAPPADARSAEVRRALGCPSQTLGAV
                     DVPADGATVSGYVVVKGYALDGNLVSNVDVYVDGTDDANRVTAAGGANINLPRPDILQ
                     AFPQYAGTPGQNPGWLASFKASNFSNGSHTIYVGVTDTTGCTYFLAPKAIKIDNTKNQ
                     APFGNMDFPLPNSSASGNGVLSVVGWALDDRKVDHVDVLVDGLLERQAVTGIYRADVA
                     ANFPDNPAAIVSGFILNVDSTRYANGVHTVTVQAVDDQGQKGFLGTVRVQVFNNAPNT
                     GPFGAVEFPLLNANWFGNCFPVSPGGPSGGPTDIVDPRILMFVNGWALEASLENTGGV
                     SEVRVALDGVPIKNSRVNCHREFLLANALIDCYGYYRPDIEVLYPGFAQAPNVGFQFF
                     VDAGYLLTQKGFREGAHILQVSAVDKRNTAVLLKEVPITMECATGQLDPPPLGYIDDP
                     TNYKFIGGVFPVIGWALDLDYVGKVRILIDGVPQIDAITGVDWADYGYASPDVALAYP
                     FYPQRNNARFRFYLDTTKLSNSEHDLLIEVSDVHGAVRSAGTRRFIVNNNTLVR"
     gene            complement(14880..15851)
                     /locus_tag="PLB01_05635"
     CDS             complement(14880..15851)
                     /locus_tag="PLB01_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HQQ76815.1"
                     /translation="MRDVPVFTDSVFLEHDTGPYHPENARRLEAVIHALRQEGRRFES
                     PPDPARTQKAIERVHEPSYVSRLKAACDAAPPGASSPFSLFDCPDNPISSATFGAAYR
                     AAGLVLAAVDAVLERRAPSVFVAARPPGHHALAAQAMGFCFFNTIAVAARDLVETSGA
                     TRVLVADFDVHHGNGTQEIFWEDGRVAYLSVHRYPFYPGTGAAEERGAGRGLGANVNV
                     PQRAGAGDGAYAGGFSAALERLAERFKPEFVLISAGFDAHEGDPLGGMAVTTAGFSWM
                     TRSLEEVAETWAGGRIVSVLEGGYDERALGESAVAHVRVLDRTGGLP"
     gene            15829..16983
                     /locus_tag="PLB01_05640"
     CDS             15829..16983
                     /locus_tag="PLB01_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQQ76816.1"
                     /translation="MKTGTSRTGRAYRTVGYDPAVTSSEKGGKALRVAVSLGLAAVLL
                     VLFLRTLDFAAVGAAIRGAHVGWLAAATACGLIATPLFRSWRWGLLLRRAGRPSAYDL
                     NSATSIGFAASTLLPARAGEIVRPVALARRAGLPVAPCLASIALERLIDLVTCVLLFV
                     VYAVGWAPQNMGGEAAGRFATLRHFALLFGAGTLAGLTVLGLLAAKPERTDRFVRPFL
                     RPLPGKIGARIEAILLSFLDGLGALGTVKDIAVVAAASLALWTLIAAQVWMTLLAFDL
                     ALPYPVSFFVIAWAILGLAIPTPGGVGGYHTAVAYALTGFYGVGKDTAGAFALVSHAL
                     SFVPITLLGLVFLLAGGLSLKALREEGRKEEDAETRAAGAKLADQDAAGE"
     gene            complement(16962..17258)
                     /locus_tag="PLB01_05645"
     CDS             complement(16962..17258)
                     /locus_tag="PLB01_05645"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQQ76817.1"
                     /translation="MRRSPRSDVRIPVALRHEGERFKGTTLVVNTHGALVGSHRNIPM
                     GDMFELENVRTRVFCHCRVVFHGGLGADGFFRLGVEMLESHPAVWGTDYSPAAS"
     gene            17356..18687
                     /locus_tag="PLB01_05650"
     CDS             17356..18687
                     /locus_tag="PLB01_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQQ76818.1"
                     /translation="MSTPTASPVLHGAARFSFWDWWVDRKTRWVRDDVHQLDEGMFRA
                     LAAGTGLPAIHQRSTFAFRNVRDGADRFLGMSKGGERPYARIYTRLGNPTSEYLERVL
                     FRLEAQHVIEKALAADETEPTIGCLIFGSGMGAISTLLLSLLKAGDAVLAGNIYGCTD
                     SLLRNMSRYGVEAVFCDTGSVAEVAKTLELHPNVAAVFLESPENPTLRLCDIEAISRL
                     TEARGIPLIVDNTFCSPWLQQPFRLGADFIVHSLTKFINGHSASIGGALLGPFRYLKT
                     DLFPWYKDLGATPSPFDSWVNSMTVQSLAVRQKAQSDSAATIAAFLRTHPKVASVTYP
                     GFHDFPQKDIVEKQMRDGGSLISFEVKGGAAAGEKLMNYLARKDVPMELAVSLGGAIT
                     YVEQPATMTHAVVPEADRLARGITPGLIRMSVGLEGPDILMQHLDTGLFLT"
     gene            18776..19177
                     /locus_tag="PLB01_05655"
     CDS             18776..19177
                     /locus_tag="PLB01_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HQQ76819.1"
                     /translation="MSEVPGEFQNALLTTPRLRREQRTIEAMLHIWCEAHPLHVRARF
                     GGLCAGCDSLLDYASYRLLKCPYGEEKPTCKKCPIHCYTKEKREQMHEVMRFAGPRML
                     LRHPILAWHHLRDEKREAPSLKRQQKPLGAV"
     gene            19192..19377
                     /locus_tag="PLB01_05660"
     CDS             19192..19377
                     /locus_tag="PLB01_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76820.1"
                     /translation="MNIELNTHESDYLKHVLDAARKSLMHELHHAQSHEFKEALKHQL
                     ELNEKLVQKIQQTLAAT"
     gene            complement(19411..>19903)
                     /locus_tag="PLB01_05665"
     CDS             complement(19411..>19903)
                     /locus_tag="PLB01_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76821.1"
                     /translation="LAFVGILAPLAACLLVQTTSRYRAGAIGPLALGSGLFLLFTLEE
                     IQRRKRRAVCLLAGTAGSLSLVPLLPSTIYDARHRWSDTLVYATLAEAERGPEAAAEE
                     IRRYVEEGGDDRAYGSAVRAFRYWVRTGERAQALVEPSGVAPPERRLREFPKTSRDAL
                     LGR"
BASE COUNT         3258 a         6947 c         6853 g         2845 t
ORIGIN      
        1 ggcgaggacc ttcttcgcag cccggcggac gagacccgcc cagtccggcg cccggcccga
       61 acgcgccgca cgcgtcagaa gcgccgtcca gtcctccggg cggatgccgc gcgtctcggc
      121 gcggcgcgcc acgaggatct ccgcccacgc cggctgctcg cgcccgtcca gcgacgccgc
      181 gacgaactcc ggggacgccc cccggcgcag ggcgaagagc aggcctgcgc cggagacgcc
      241 ggtcttctcg agcaacagga gcgcgacgcg cccgcgcggg ccgtcctcca gctcgcggaa
      301 gacgccctcg acgagcgcgt ggcccgacgc gaagaagtcg aggtcctcct tttcgacggc
      361 ctcgtcgcgg tcgaacgttc cgagccagcg ggacccgccg gagacgccgg gcaggtgctc
      421 gacgagagcg tccgcgccga actcgagata ccacgtgcgc cggcccgagc gctcggccgt
      481 ctcgaagccg aagaggtccg cggcctcgag gacgaagcgc tccgtacggg cgtcgagatc
      541 ggccgggacc cgcgcgagga ttccggccgc cttctcgggc gcgtaaccgt cgcggtgcag
      601 gtggtgatag acggccttct gccgcgcctc cgccgcgcgc gccacctccg ccgccaggtc
      661 gtccaccggc agcggctttc cgagccgccg cgcacgcgtg atgcccgcct ccacgccggc
      721 gagcgagcgc tcgaggccgc cgagcggcga cgtaaagagg ccgatgcgct cgtagagcgt
      781 cgcgagctcg cgcccgaagc cgtccggcgg ccggaagtag acgatctcga ccggagacgt
      841 gcgattgatt cggtcgaggc ggccgatgcg ctgctcaatg gccgcagggt ccgcgggcag
      901 gtcgaagagg acgaggcgcc ggcagaactc gaagttgcgt ccctcgccgc ccgcctccgt
      961 cgagacgagg atcgacgggc cgtccgcgcg ccggaactcc gccacctcga cgtcgccgcg
     1021 ctcgggcgtc agctcctcgt ggaagatcgc gactcgcttg cgcgtggccg actcgagccg
     1081 cgccttgagc gccgaaagcg tcgcgcgttc gtggacgaac acgagcgtct tggccgagcc
     1141 gtccgcgcgc cacgcggcgt cctctcccgc gatccacgcg acgcgcgggt cgtccgcgtc
     1201 cgccgcgtcg tcgcccgtca ggccctcggc cgcgggcacc cgaccgaggt cgacgggccg
     1261 cgggacgcgc ggcatgaggc cgccgatgtc cacgcgccgc gtcgcgctcg tgcagggcgg
     1321 cagcggctca tccttttcga gggaggcgag gaacgcctcc tcgctcgtgt acgcgtcggg
     1381 gcggaggagc tcgaggagac ggaagaaccc gtgcgcgtcg gcctcgagcg gcgtcgccga
     1441 gaggagcagc acgtgcctgg ccgccctcgt cagcggcgcg acggcgagat acgcgtcgct
     1501 tcccgcctcg ccccgggcgc gctcgaggcg gtgggcctcg tccacgacga ggaggtcgaa
     1561 gccggcggcc tcggccttcg cggcgagacc ggtctctccc gccaggtcct cgagggcgac
     1621 gatcgcgtgg cggtgcgcct cgaatgggtt gaatccctcg ccatgctccc tcgcgacgtc
     1681 ctcacggcgc ctgcggtcga ggagcacgaa cgtctggtgg aacttgcggt agagctcgcc
     1741 gagccactgg accgtgagcg tggacggcgc gacgacgagc gcgcgatccg cgcgatccgt
     1801 gcgcaggagg cgcgagagga cgaggcaggc ttcgatggtc ttgccgaggc cgacctcgtc
     1861 cgcgaggagc caccgcacgg ggtcgctcgc cgcggcccgc tcggccacgt ggagctggtg
     1921 cggaaagatc gcgatccgtc cgccgaggaa cgacccgagg ccgcgggcct ggcggatctc
     1981 cgagaggagg aggccgtcga cgcggttgcg gaacgccgcc gcccggtcgg tctgcagggc
     2041 gccgaggcgg tcgatcgggt cgtccggcgc cgcgagcggc cagagccgcg cggacgtcac
     2101 gacccgcccg tccgcgagac gcgacgtctc ggcgccgacc tcgtcgacga cgaccggttc
     2161 ctcctcgccg tcgaggcgcg ccttctgccc ctcgcgcagc ggcgccggac gcagggcgtt
     2221 gtcgtcggcc gcgagcgtca ggcgcgtgtc cgccgcgggg aaccacacga cgatccgccg
     2281 cccgtccacc tgctcgacgc ggcccggccc gaggtccggg ttgaacttgt ggaagagctt
     2341 gtcgtgttcg cgaaacgccg gcacgggggc gcccatggtg ccggttgaag acgattcggg
     2401 caagtgccgt cgaagcggat ctcaagcgga agggcccggg gtcccgcgat ctggccgcgg
     2461 gggcgcggat ctgcggggga ggaggaggga tcagcgagca gacgccgtgc tgagctgcct
     2521 cttaaggtcg ctttcgtccc agtccgccgt ggacgccacg atcttgccgt ccacgatctt
     2581 catcttcgtg gagccctgga tgttcacctt ccggcccgtc ggcgccagcc cgccgaggga
     2641 tccgcggttg gtgccgtggg ccgtccactt cacgtgggtc tcgtctccga ccttcgtgat
     2701 cgacgcgacg tcgacgcgta ggtccgggaa cgcctggcga aaccgttgca ggagcctttg
     2761 gcactcggcg gccgtcgcgg gatgcgatgc gatcaccgcg gtgttcccct cgctccagcc
     2821 ctgtccgtag aaggcgcgga ccactttctc ttcggcaggt gtcgggtgaa tcgattgggg
     2881 cagccgcgcc ggcgaggacg tcgtgggcga cccgacgagg gcacggagcg gcgcgaggag
     2941 cgcgctcgcc gcagcggcaa agccgagttt gagaacggtg cgccgcttct ccacgagcca
     3001 cctccctgcg gacgacgacg agtaattctg gggaggctga gactccgtgt caagatgatg
     3061 cccgggcgcc agcggccggc acggcgggat cttctgcaag aatgtgccgg gggcggcacg
     3121 aaacatggac ctcgaaccgc atccgttcaa cacggggttc gcctggtccg aggtgaaggg
     3181 ccctttcagg cgtctctccg ccgctgaggc gcaatccttc aacgagcgcg gatacgtcgt
     3241 actcgaggac gtgttcgacg ccggggtgct ccgtgctctc gtcgacgtga tcgaccccgt
     3301 cgaagctcgc gtcgaggcga tgctgagagg gcagcccggc cagaggatat tcgtcgcccg
     3361 ggccggggag atcaccttca cggtccatct cgcgagcctt tcgcctctcg tgcgtgcgtt
     3421 ctgctccggt ccggtcttcc gggaacttgc gcacgacctg gtcggtccgg acgtacggct
     3481 ttactgggac caggccgtgt acaagaagcc gtcgatctcc ggagatttgc cgtggcatca
     3541 ggacaacggc tatacgttcg tcgagccgca gcagacgctg acgtgctgga tcgcgctgac
     3601 ggacacgacg gaggagaacg ggtgtctctg gctcgcgccc ggcctccacc ggttcggcac
     3661 gctccgccac tggaggacgg agcagggctg gtgttgtctg aaggagccgc cggagaccgc
     3721 tgcggctcca ctgcgtgccg ggagcatggc ggtcttctct ggtgtgaccc cgcatcgaac
     3781 agggcccaac ctcaccgacc gggtgcgcaa ggccttcatc ctgcagtttg cgaaggacgg
     3841 cgcatgcacg gtcgcaccgg acccggggac cggcgaacca gtccgccgtc cctgcaacga
     3901 ccctcgctgc cagtttcccg ttctcgcagg cggcgaagcg ccctcactct cccgatagag
     3961 gaccgtccga aacctcgctt cgccccgacc gcgcgcgcgc gacggcagcc tcgaggtccc
     4021 tcagtgtggc gtcgggagag accgacgagg ggtcgaacga aacgcggaac ttcgcctcga
     4081 ggcggaggat cagctccacg cggccgaggg agtcgaggcc ctggcccgcg aggggcctct
     4141 cgggatcgag ggagcgcccg ggcgcattca ggacggatgt cacctcgcgg gcgaggtcgt
     4201 ccagaatcgt cggggaaagc gccgccgggg tcgcgacggg cgagggccat tcgtcgacga
     4261 cgtcccacgt ccggtcgaga aacgcctggc ggcaggcgcc acgacggatc ttgccgctcg
     4321 tcgtacgcgg cagttcgccc tgcttcagga tctggaccgc ggacaccgcg acatccatct
     4381 ccgaagcgac ctcatcccgg atgctgcaga ggaggaccgc cgtatcgagg ccaggcgcgg
     4441 cttcgcgcgg catctcgcag gcgacgacga gccgctcgcc ctccaggccg ccgacggaaa
     4501 aggcggcgca cctcgttgga gacagcgcgg cgtgactccc gccggcgcac gcctcgatgt
     4561 cctggggata gtagttcctg ccaccgagga tcacgaggtc cttcagccgc cccgcgacga
     4621 agaggcggcc gtcccggatc aagccgaggt ctcccgtgcg aaggaacagg cccgggcggc
     4681 ccggaaggcc gttttcgaac gcggcatggg tcgcctcggg ccgtttccag tacccgcgtg
     4741 cgacactggt ccctgacagc cagatctcgc cggtctcgtc ctcgccgcat gtgcgaagcg
     4801 agcccgggtc gacgacagcc acctcgattc ctcttgccgg cgtcccgcac gatacgaagc
     4861 gccgcgcacc aggttcgaat gcctcgacgc gcacgccgga ttcgccggac acgagcaggg
     4921 tcgactccgc gagcccgtag cacggaaaga aggcatcggc gcggaagccg catggcgcaa
     4981 agaacgcggc gaaacgctgc atggtttccg cacggaccgg ctcggacccg ttgaacgcga
     5041 tccgccacga accgagatcc aacccctcgc gctcttcggg caagaccata cgcacgcagt
     5101 gcgcgaacat cgaatcgggg gcgccgctcg tcgtggcccg tgtgcggctg atggcccgca
     5161 gccagcggat cggcctctcg accgcgtgct tcggcgccat cagcgtcacg tcggaaccga
     5221 gcgcgatcgg ctgcaggatc gcgccgatca ggcccatgtc gtgatacggc ggcagccaac
     5281 tcacgaagcg cgagttctcg tcgtggccca tcgcctcggc gatcgcgtgc tggttctcca
     5341 tcaggttccg gtgcgagagt gcgacgccgg ccggagcgcg cgtagagccc gacgtgtact
     5401 gcagcatcgc gatggcgtcc ggctcggcac cgaggtcggc gcgctcgacg cgtagggtga
     5461 atcgggagtc gtcggtcgcg agccactcga gcgaaggcgc aacgtgcgtg tccgccgttt
     5521 cgtggcggca ggagcccagg tgcgcggcgt cggtcagcgc gagcgccggc ctcgcgtcct
     5581 cgacgacggc ggccagccgg atgtctgcgc ggccgcgtcc cgaccggggc ggacaggtgg
     5641 gcaccgcaat cgcacccgcg ttgaagcacc cgaaaagggc cgcgatgaag tcgaggcccg
     5701 ggggatgcgc gagaaggacc gtccgacccg cgaggtcgcg acgccggagg gcggacgcga
     5761 ttccggcggc gcgtccgtag agatcagagt aggtccacga gacgcttcgg gtctcaccgt
     5821 cctcgaggaa ggtgaccgcg gtcttgccgg ggcgctcgac cgcgtgagcc gcaagaacgt
     5881 cgacgagtgt cgcgaagcct gcccgcacgg cgggacctta cctctagccc agagcctgat
     5941 ccaggtccgc gatcaggtcg tccgggtgct cgaggccgat tgaggcgcgc aggaggtcct
     6001 gcggcgtcgt agaacccggc ccctcgctcg tcgcgcggtg ctcgaggagg ctctcgacgc
     6061 agccgagcga cgtcgcccgc acgaagagct tcgccctccc gaccgccgcg acggcggcgt
     6121 cgcgtccgcc cttcacgtgg aaggacagca tcccgccgaa gcccgacatc tgcttcgccg
     6181 ccacggcgtg accggggtgc gacgccaggc ccggatagtt cacgcgcgag accctcgggt
     6241 gcgcctcgag ggcgcgcgcg acggcgagcg cgttcgcgct gtgggcgttc atccgcacgg
     6301 ccagcgagcg cagcccgcgc aggaccagcc acgcgttgaa cggcgcgagc acggcgccga
     6361 gcagatgcct gacgtgcgcg actttcgcga acacgtcgtc cttcctgcgg aagacgagcg
     6421 cgcctccctg cacgtcgctg tggccgccga agtacttcgt cgccgaatgg agcgcgacgt
     6481 cggcgccgag gtcgagcggc cgctggaggg cgggcgtcgc gaacgtgttg tcgacgagcg
     6541 tgagggcgcc cgcccgcttc gcgagcgcgg cgatcgccgc gaggtcggcg accttcatga
     6601 gggggttgga cggcgactcc acgaggacca gcttcgtctc cggccggatc gccttcgcga
     6661 cggcggcgag gtcgtccatc gcgcaggatg acgcggtgag gccccacgcc ggaaagaact
     6721 cctgcccgac cgccctccag ccgtagtacg cgtcgtcggg aaggaggacg tgcgcgccgc
     6781 gcggaagcgc ctggaacagc gcgaccccgg ccgccatgcc cgagccgagg acgagggccc
     6841 cttcgccgcc ctcgagcggc gcgagagcgt cctcgaggag cgtctgcatc ggattcgact
     6901 cgcggatgta cgtgtggccg ccgagaggaa cgcccgtctc cccgcgctcg aacgtcgtcg
     6961 agaggtagat gggaggcgcg acggagcccg tccccgcctc cggtctccgt cccgcatgaa
     7021 ccgccagcgt ctcgaacctc atgggtcctc cgacggaggt gtggcaccat ccccgcgtga
     7081 tgcggctccg gacctccgcg ttcctgctcg ccactctgat cgcgttgccc ctcgccgcgc
     7141 aagaccccac gccgtccaag ctggacgtcg gcccgggact caagcgcgag cgcgagcgcc
     7201 tctcgaacta ccagtggcgc ctcaagaccg agatgagggt ggacgggacg ctccggatgt
     7261 tgaaggtcga ggacgtccac ctcgggcccg acgggggcct cgtcaagaag acgatcaagt
     7321 tcgacaagcc ggccgagccg actccgttcg ccgagaacga cccccgggcg cgggtctacg
     7381 cgccgacgac cggcaccgag gaagagcgcc tctcggagat cgcgcaggac ctcatgcagt
     7441 actacgcgcg cctctccccc gagcgcgtgg ccgagtgggc caagaacgcc gaggttcttc
     7501 cacccgaggc ggaccacccc ggcctcgtgc gcatgcacgg ccgcggtctc ggccgcccgc
     7561 tggacgacgc ggtgctcttc ttcgacccga agacgaagac cgcggcgcag atcgaggtca
     7621 agacgaccgt cgacccgcgc atcctggaca tcgccttcat ccgcgcgacg ttcgagccgc
     7681 tcccgcaggt gcgccccgac gtcgcgccga tctgggtgcc gaaacgcgtg ttcctgaaca
     7741 tgaggcgcgg caagcgcgcc gtcgccctgg agatggaaac gaccgactgg cgccagtggc
     7801 cctagccccg aagggggcgc gggggaaacg ctggaagggt ttcccccgcc ctaaagcttt
     7861 ggaacccctt ccggatggtt cctcaggaac acggtgcttc cgacgagcag gagcagagcc
     7921 ccgttcccgg cgaggaccca gggcgccggc atgacgccga cgaggaagcc cgccgccagg
     7981 ttgcccagcg gcatcgcgcc ccggaacgcg agcgagtaga cgctcacgac gcgcccgcgc
     8041 atggcgtcgt ccacgttcgt ctggacgagc gtcatgaaca tggcgaagac gatcatgagc
     8101 gccgcgcccg cgaggaagag cagcggaaac gagagccact tcgtccgcga gagcgcaaaa
     8161 ccgatcatca gcgccccgaa cgccatctgc atcgcgaccg cgaccgcgcc gcgccgcttc
     8221 acgtgcccga agccggccgc cccgatcgcg ccgctcaccg ccccgaggcc gaacaccgcg
     8281 agaaacgtgg ccaggacctt cacgtcgccc ttgaagacgt ccttcgcgaa gacgggcagg
     8341 aacgtgagga gcgggaacgc gcagaagctc ccgatgaacg agagggcgat gagccctcga
     8401 agcgcgcggt cccggtgcgc gcccgcgagg cccgccttga ggttctccat cacgctgccg
     8461 ctggccgcgc ccgtgggggc cccgcgcctg agcgcgagga gcgccaggat caccgcgaag
     8521 aacgacaggc cgttcagccc gaagcacgcc gccgcgccga gcgtcgcgaa cgccgcgccc
     8581 gcgaggaccg ggccgatgac gcgcgcgagg ttgaactgga tcgagttgag cgcgaccgcg
     8641 ttcggaaggt cttccttgtc cacgagggtc gggacgagcg cctgatacgc cggcccgccg
     8701 aacgactgcg cgattccgac gacgagggac aggacgagaa tcatccagac ctggacgtgg
     8761 ttcgcataca cgagaaacgc gaggaggaac gccgtcgtga gctggacgac ctgggagagc
     8821 agcaggatcc gccgccggtc catccggtcc gcgagaacgc cgccgaccag cgagaagacg
     8881 aggaacggcg cctgctgaag gccctccgtc cacgcgaggt actgcggatt gcccgagatc
     8941 gtcaggacga gccagctctg ggcgaccgac tgcatccacg tgccgacgct cgacgtgaac
     9001 gcgccggccc acagcaggct gaagtcgcga tacgtgaacg ccttgaagac tctcgtcagc
     9061 ctgtcgcgca ccgcgagcac catacctcag cgaatgagtc tcgtcagttt tccgtgggcg
     9121 ccgcgtcctt cggaacgcgt ttcttgaaga cccacgccat ggcgactccg aggaggtaga
     9181 gcccgcacat cgggagggcg aacgcacact gggtcgggat gtcgggcgtc ggcgtgatga
     9241 gagccgcgat gatgaagatg acgaggacgg cgtaccggaa cttcgcgagg agccatttct
     9301 ccgtcacgac gcccatgcgc gcgaggaaga agatcaggat cggcgtctcg aagacgagcc
     9361 ccatcccgag aaggatcttc gacgtgatgt cgaggtagtc gtcgatcttg atgacgggca
     9421 tgaagtcctc gccggtcgcg acgaggaact gcgccaccat cgggaatccg acggcgtaac
     9481 cgaagtagcc tccccccacg aagaagagga ccgacaggac gacgaacggc cagacccacc
     9541 ggcgttcgtg cttgtagagg cccggggcga tgaagagcca gagctggtag aggatcaccg
     9601 ggctcgccac gaacacgccc gcgaggaggg cgatgttgat gtagagcatg aacggctcgg
     9661 tgagctgcgt gtacgcgagc ttcgtcccgg gcggcagcgc ggcgaggacg ggcttcgcga
     9721 gccaattgaa gatcggcttc gccttccacc agcagaccat gaagccggcg gccacggcga
     9781 acgcggacca gaagaggcgc ttgcggagat cctcgaggtg ctcgaggaag gacatccgcg
     9841 ggaggtcttc ctccggatcg cctccggggc ggcgcgaggc gtcggtcacg gggcgggcgt
     9901 cttcgtcgtc tcggacgggc gggagatcgt cccgtcggac ggggcgagcg cgggcgcgga
     9961 attctccggc tcggttttcg gcggatcgtc gatcttcacg gccgaggacg ccttttccat
    10021 ctcgtcctcg atcgtgcgct tgaggtcgtt cgaggcgcgc ttgaactcgc cgagcgcctt
    10081 gccgacctgc ttgccgatct cggggagctt cttggggccg aagagaagca gcgccagaac
    10141 catgatcatg atcagttccg gcatcccgag attgcccatc ccgtcccctc ctctggagcg
    10201 ccgatgctcc gctcggcacc gggcgaagtc aaggcgtcgc ccttcccctg cgtgctagtc
    10261 tcattgcgtc cgggagggtc ccttcacgtg aacaacagcc ccgtttcccc gcgttcccgc
    10321 tccatccggc atgccctcgc cgccgccgtc ttcctcctcg cggtcacggt cggcggaggg
    10381 atcgccggcg ggcgcctggg cgccgtctcg gacgcgacga accccggttt caaggaatac
    10441 tccgacatcc ttgccacggc ccgggcctgg tacgcggacg aagtcccggc aaacaagctc
    10501 gtctatgcgt cgatccacgg gatgctcggc acgctcgacc cgcacacgaa cttcctcgag
    10561 cccgaggagt actcctcgat ggtcgagaag cagcgcgggt ctttctacgg gctcggaatc
    10621 atcatcagca agcgcaacgg gaaggtcacg gtcatcacgc ccgtcgaggg ctcgccggcg
    10681 gaccgtctcg gcatccgcgc gggagacgtg atcgacaagg tggaagggca gccgatcgac
    10741 gaccttccgg tcgacgcggt cgtcaagaag ctcaagggcc ccaagggcac caaggtgcgc
    10801 atcacgatcc tccggccggg cctcgacgag cccctccaga tgaccgtcac ccgcgcggag
    10861 atcccgacga actcggtctc gtatgcgttc ctcgtcgagc cgggcgtcgg ctacctccgc
    10921 ctgaaggact tcacgcacac ctcagccccc gagctcgccg agaactggga caagctcgaa
    10981 aagcagggaa tgaagaagct cgtcctcgac ctgcgcggca acccgggcgg cctcctcgac
    11041 caggccatcg ccgtgtcgga cttcttcctc tcgcgcaacg agaaggtggt cgtgacccgc
    11101 ggccgcaacg gcgcccagga gcagagcttc gcggcgcccg ggaagaacac ccacgcccgg
    11161 atccccgtcg tcgtcctcgt gaacaaggga tcggcctccg cttcggagat cgtcgccggc
    11221 gccctgcagg accacgaccg cgcggtcatc gtcgggcaga cgacctgggg caagggcctc
    11281 gtgcagtcgg tctacaacct ctcggacgga gcgggcctcg cgctcacgtc cgcgaagtac
    11341 tacacgccct ccggccgctg catccagcgc gactacaagg acgtcctcga gtacctctcc
    11401 cacgaggacg aggagggcgg cgacgccgaa gagacgggtc cggcccgcga cgcggcgtcg
    11461 tctcagaaga aggagtcctt caagacggac gcgggacgca cggtctacgg cggcggcggc
    11521 atcacgcccg acgtcttcgt gaaggcgccg acgctctcgc gcttcgcgac gacgctctat
    11581 gcgcgcgggc tcttcttcga gttcgcggtc gactacctcg cgaagcactc gacggttccc
    11641 cgcgactttc ccgtgaccga ggccgtcagg gacgacttct tcaagttcgt cgacgcaaag
    11701 ccgggcatcc ttctcgaaaa gagcgcgaag gcgacgtacg ccgaggagaa ggaccccctc
    11761 gtcgtcgacc gcgcgatccg cgaggaagtc ctgactgcaa agttcgggcg cgacgcgggc
    11821 taccgggcca gcctcgagaa cgacgtccag ctcaagaagg cgctcaccct cttccccgag
    11881 gccgagaagc tcgcgaccgc gcacgagcag atgcagctcg cgaagaagtc cgagttgaag
    11941 ggtcccctga agccgtaggc cggatcagga gccaccagag gacgaacggc gccgcgaagc
    12001 agcccgcgag acgccagaag tgggacgcgg tggcccagaa gttccgcgca aacggaccgt
    12061 acgccgccgc gctccacgcg ccgagcttga gcgcgtagaa ggactgcacg gcagcgacga
    12121 gcgccgcgac gtgcagggcg aagagcacga cgagcgagat cccgagcccc ctcatcgcgc
    12181 ggtcccggaa gaggccgggc gtcgcggccg ccagcgtgaa aaggatgatg acgtcgaacg
    12241 tgaggtcgtc cgccggaagc gacggccggt cggatcggga cgtgaagtcg gcccggtcga
    12301 tcacgacgcg cgcttcctcc gcgtagagcc gggtcgccgc cggccgctcg agcaggcgta
    12361 cgaccggctc cgcgaagccg gcgagcagcc ggttgtaggc gggtgtcccg gcgcgccaga
    12421 ggccgagccc gagcgcgaag cccagggcgg ccctaagcgc gagcttcagc actttctccg
    12481 ccgcgtagct tccggcccca cgcgaggaag atcccgactg cgagcaggat gaggagcgtc
    12541 tgccagatcg ccgcgtggaa cagctcgaac acgtcgcgcc ggtgggcgcc gagccagaag
    12601 agcgacacga tccggaacag gttcacgacc tggatgacgg cgagacccgt cgcgatgccc
    12661 gcggcgcggg cctttgccga ggccggaaac gcgagcatcg cggcgaggag gaggagcgcc
    12721 gcctcgatcc cgttgcagcc gttcttgacg tccaccgcga agagcgccga ccggatcacc
    12781 gtgccgctcg tgacgacggg ctcgccgacc gcgcggagga tccccgccga caccttcacg
    12841 aggaaccccg tgaacggcac gacgacggcg tcgttgacgg gctggatcgc gacgagcgcg
    12901 tagagcccca cgaggaacag cgcgaaccgg atcaggaact tcttctgggc gggctgcatg
    12961 gcgagcgacg aggataaatg aaaaggcccg gcaaccgccg ggcccccgct ctcttacctt
    13021 ctaagaaaat ctcttcgtct tcttttcgtc ttctcttctt cagcggacga gggtgttgtt
    13081 gttcacgatg aaacgccggg tgccggccga gcgcaccgcg ccgtgcacgt cggagacctc
    13141 gatcaggagg tcgtgctcgg aattcgagag cttcgtcgtg tcgaggtaga agcggaagcg
    13201 cgcgttgttg cgctgcgggt agaagggata cgcgagcgcg acgtccgggc tcgcgtagcc
    13261 gtagtccgcc cagtccactc ccgtgatggc gtcgatctgc ggcacgccgt cgatcaggat
    13321 ccggaccttt ccgacgtagt cgagatccag ggcccatccg atcacgggga agacgcctcc
    13381 gatgaacttg tagttcgtcg ggtcgtcgat gtagccgagc ggcggcgggt cgagctggcc
    13441 cgtcgcgcac tccatcgtga tcgggacttc cttcaggagc acggcggtgt tccgcttgtc
    13501 gacggccgac acctggagga tgtgcgcgcc ctcccggaag cccttctgcg tcaggaggta
    13561 gccggcgtcc acgaagaact ggaagccgac gttcggggcc tgcgcgaagc ccggatagag
    13621 gacctcgatg tcgggacggt agtacccgta gcagtcgatc agggcgttcg cgaggaggaa
    13681 ctcgcggtgg cagttcacgc gcgagttctt gatcggcacg ccgtccagcg cgacgcgcac
    13741 ttccgagacg ccgcccgtgt tctcgagcga ggcttcgagg gcccagccgt tgacgaacat
    13801 gaggatgcgc ggatcgacga tgtccgtcgg gccgccggac gggccgcccg gcgagaccgg
    13861 gaagcagttc ccgaaccagt tcgcgttcag gagcggaaac tcgaccgcgc cgaaggggcc
    13921 tgtgttcggt gcgttgttga acacctgcac gcgtacggtc cccagaaagc ccttctggcc
    13981 ctggtcgtcc acggcctgga cggtgaccgt gtggacgccg ttcgcgtacc gcgtcgaatc
    14041 gacgttcagg atgaagcccg agacgatcgc ggcggggttg tccgggaagt tcgcggcgac
    14101 gtcggcgcgg tagatgcccg tgacggcctg ccgctcgagc agcccgtcga cgagtacgtc
    14161 gacgtggtcg accttgcggt cgtcgagcgc ccaacccacg accgagagga cgccgttgcc
    14221 ggacgccgag ctgttcggga gcgggaagtc catgtttccg aacggcgcct ggttcttcgt
    14281 gttgtcgatc ttgatcgcct tgggcgcgag gaagtacgtg cagccggtcg tgtccgtgac
    14341 cccgacgtag atcgtgtggg atccgttcga gaagttcgag gccttgaagg acgcgagcca
    14401 accggggttc tggcccggag tgcccgcgta ctgcgggaag gcctggagaa tgtcggggcg
    14461 cggcaggttg atgttcgcgc cgccggccgc cgtgacgcgg ttcgcgtcgt cggtgccgtc
    14521 aacgtagaca tcgacgttgg acacaaggtt gccgtccagc gcgtacccct tgacgaccac
    14581 gtacccgctg acggtcgctc cgtccgccgg aacgtctacg gctcccagcg tttgcgaggg
    14641 acatccgagg gcgcgccgga cttccgcgct gcgggcgtcc gccgggggcg ccatgagcag
    14701 ggccacggcc gcgacggtcg ccgagaggat cagggccttc cgaatcatca cgagcctccg
    14761 tgcgtcgaga agaaattcca aggcacgctc ggacaccgaa tcatgaagag agattgacgc
    14821 gtattgagtc taacgggcgc ggaaggctct gtcaaatgag cgatttacgc gaggctcccc
    14881 tacggcaggc cgcccgtccg gtcgaggaca cgcacgtgcg cgaccgccga ctcgcccagc
    14941 gcacgctcgt catacccgcc ctcgagaacg gagacgatcc tcccgccggc ccaggtctcg
    15001 gcgacctcct cgagggagcg cgtcatccag gaaaacccgg ccgtcgtgac cgccatcccc
    15061 ccgagcgggt cgccctcgtg ggcgtcgaag ccggccgaga tcaggacgaa ctccggcttg
    15121 aacctctccg cgagcctttc gagggccgcg gagaagccgc ccgcgtacgc gccgtcgccc
    15181 gccccggcgc gctgcggcac gttcacgttc gctcccagcc ctcgccccgc cccccgttcc
    15241 tccgcggcgc ccgtgcccgg gtagaacgga taccggtgga ccgacaggta ggccacgcgt
    15301 ccgtcctccc agaagatctc ctgcgtcccg ttcccgtggt ggacgtcgaa gtcggccacg
    15361 aggacgcgcg tcgcacccga cgtctccacg aggtcacgcg ccgcgacggc gatcgtgttg
    15421 aagaaacaga agcccatcgc ctgcgcggcg agcgcgtggt ggccgggcgg ccgggcggcc
    15481 acgaacaccg acggcgccct ccgttccagg accgcgtcca cggccgcaag gacgagcccc
    15541 gccgcccgat acgccgcccc gaacgtcgca gacgagatcg ggttgtccgg gcagtcgaac
    15601 agcgaaaacg gcgaggaagc gcccggcggc gccgcgtcgc aggccgcctt caggcgcgag
    15661 acgtaggagg gctcgtgaac gcgttcgatc gccttctgcg tgcgcgcggg gtcgggcggg
    15721 gactcgaacc gtcgcccttc ctggcgcaac gcgtggatca cggcctcgag ccggcgcgcg
    15781 ttctcggggt ggtacgggcc ggtgtcgtgc tccagaaaga cggaatcggt gaagaccggc
    15841 acgtcgcgca cgggacgagc gtaccgcacg gtgggctacg atccggcggt gacctcctcc
    15901 gaaaagggcg ggaaagcgct gcgggtcgcg gtgtccctcg gcctggccgc cgtccttctc
    15961 gtgctgttcc tccggacgct cgacttcgcg gccgtggggg cggcgatccg gggggcgcac
    16021 gtggggtggc ttgccgccgc aacggcctgc ggcctcatcg cgactcccct cttccgctcg
    16081 tggcgatggg gactgctcct ccggagggcg gggcgcccct cggcctacga cctcaactcg
    16141 gcgacgtcga tcggcttcgc ggcgtcgacc ttgctgcccg cccgcgccgg cgagatcgtc
    16201 cggccggtcg ccctcgcgcg ccgcgccggg ctgcccgtcg cgccgtgcct cgcgtcgatc
    16261 gcgctcgagc gcctcatcga cctcgtgacg tgcgtgctcc tcttcgtcgt ctacgcggtc
    16321 ggctgggcgc ctcagaacat gggcggcgag gcggccggcc gcttcgcgac cctccgccat
    16381 ttcgcgctcc tcttcggcgc cggaaccctc gccggcctca ccgtcctcgg cctgctcgcc
    16441 gcgaagcccg agcgcacgga ccgcttcgtc aggccgttcc tccgtccgct gcccgggaag
    16501 atcggcgcgc ggatcgaggc gatcctgctt tccttcctgg acggactcgg cgccctcggc
    16561 accgtgaagg acatcgcggt cgtcgcggcc gcctccctcg cgctctggac cctcatcgcc
    16621 gcccaggtct ggatgacgct tctcgccttc gacctcgcgc tcccctaccc cgtctcgttc
    16681 ttcgtcatcg cgtgggcgat cctcggcctc gcgatcccca cgccgggcgg cgtgggcggt
    16741 taccacacgg cggtcgccta cgcgctgacg ggcttctacg gcgtcggcaa ggacacggcc
    16801 ggcgcgttcg ccctcgtctc gcacgcgctc tcgttcgttc cgatcacgct cctcggcctc
    16861 gtcttcctcc tcgcgggcgg gctcagcctg aaggccctgc gcgaagaggg acggaaggaa
    16921 gaggacgccg agacgcgcgc cgccggggcg aagctggcgg atcaggacgc tgccggagag
    16981 tagtcggttc cccacacggc cgggtgcgac tcgagcatct cgacgccgag gcggaagaag
    17041 ccgtcggcgc cgagcccgcc gtggaacacg acccggcaat ggcagaagac gcgcgtccgc
    17101 acgttttcga gctcgaacat gtcgcccatc gggatgttgc ggtgggagcc cacgagcgcg
    17161 ccgtgagtgt tcacgacgag tgtcgtgcct ttgaacctct ccccttcatg gcgcagcgcg
    17221 accggaatcc gcacgtcgct gcgggggctt cggcgcattt ctgtccgagg actgggctcg
    17281 gccacggacg ctccttgggc cttcgaacgt gcaccattct agccccggag cgccgcgggg
    17341 gctaaattcg ggggcatgag caccccaacc gcctcccccg tcctgcacgg cgccgcccgc
    17401 ttctccttct gggactggtg ggtcgaccgc aagacccggt gggtccgcga cgacgtccac
    17461 cagctggacg agggcatgtt ccgggcgctc gcggcgggca ccggcctgcc cgccatccac
    17521 cagcgctcga cgttcgcgtt ccgcaacgtc agggacggcg ccgaccgctt tctcgggatg
    17581 tcgaaaggcg gcgagaggcc gtacgcgcgg atctacacgc gcctcggcaa cccgacgagc
    17641 gagtacctcg agcgcgtcct cttccggctc gaagcgcagc acgtcatcga aaaggcgctc
    17701 gccgccgacg agaccgagcc cacgatcggc tgtctcatct tcggctcggg aatgggcgcg
    17761 atctcgacgc tgctgctgtc gctcctcaag gcaggcgatg cggtcctcgc cggcaacatc
    17821 tacggctgca cggacagcct cctgcgcaac atgtcgaggt acggcgtgga ggccgtcttc
    17881 tgcgacacgg gcagcgtggc cgaggtcgcg aagacgctcg agctccatcc gaacgtcgcg
    17941 gccgtgttcc tggaatcgcc cgagaacccg acgctccgcc tctgcgacat cgaggccatt
    18001 tcgcgcctga ccgaggcgcg cggcatcccg ctcatcgtcg acaacacgtt ctgctcgccg
    18061 tggctgcagc agccgttccg gctcggcgcc gacttcatcg tccactcgct gacgaagttc
    18121 atcaacggcc actcggcgtc catcggcggc gccctgctcg ggccgttccg gtacctgaag
    18181 accgacctct tcccctggta caaggacctc ggcgcgacgc cctcgccgtt cgactcgtgg
    18241 gtgaactcga tgaccgtgca gtccctcgcc gtccgccaga aggcccagag cgacagcgcc
    18301 gccacgatcg ccgcgttcct gcgcacgcac ccgaaggtcg cgagcgtcac gtacccgggc
    18361 ttccacgact tcccgcagaa ggacatcgtc gagaagcaga tgcgcgacgg cggctcgctc
    18421 atctcgttcg aggtgaaggg cggcgccgca gccggcgaga agctgatgaa ctacctcgcc
    18481 cgcaaggacg tcccgatgga gctggccgtg agcctcggcg gagccatcac gtacgtggag
    18541 cagcccgcga cgatgacgca cgcggtcgtc cccgaagccg accgcctcgc ccgcggcatc
    18601 acgccgggcc tgatccggat gagcgtcggg ctcgaagggc cggacatcct catgcagcac
    18661 ctcgacacgg ggctcttcct gacgtgattc cccattcgta tgacgtgcgt catgtccggc
    18721 tcttgatgca ggtgcatcga tgatgcaaat tgccgggatg agacaggatc tccccgtgag
    18781 cgaagtcccc ggcgaattcc agaacgccct gctcacgacg ccccgcctgc gccgcgaaca
    18841 gcggacgatc gaggccatgc tccacatctg gtgcgaggcg caccccttgc acgtccgcgc
    18901 gcgcttcggc gggctctgtg cggggtgcga ctccctgctc gactacgcga gctaccgcct
    18961 gctgaagtgc ccgtacggcg aagagaagcc gacgtgcaag aagtgcccga tccactgcta
    19021 cacgaaggaa aagcgcgagc agatgcacga ggtcatgcgt ttcgcgggcc cgcgaatgct
    19081 gctgcgccac ccgatcctcg cctggcacca cctgcgcgac gagaagcgcg aagccccgtc
    19141 cctgaaaaga cagcaaaagc cgctcggcgc ggtctaggag acgagcgtcc catgaacatc
    19201 gaactgaaca cacacgagag cgactacctc aagcacgtgc tcgacgcggc gcgcaagtcg
    19261 ctgatgcacg agctccacca cgcgcagagc cacgagttca aggaagccct gaagcaccag
    19321 ctcgagctga acgaaaagct cgtccagaag atccagcaga cgctcgcggc gacgtagaag
    19381 accgccgggg acaggcggcg aaatcccggg tcagcgcccg agaagcgcgt cccggctcgt
    19441 cttgggaaat tccctcagcc ggcgctccgg cggcgctacc cccgaaggct cgaccagcgc
    19501 ctgcgcgcgc tcaccggtcc ggacccagta gcggaacgca cggaccgcgg atccgtaggc
    19561 ccggtcgtcg ccgccctcct ccacgtaccg ccggatctcc tccgccgcgg cctcggggcc
    19621 gcgctcggcc tcggcgagcg tcgcgtacac gagcgtgtcc gaccagcgat ggcgggcgtc
    19681 gtagatcgtc gagggcagga gcgggacgag agacagcgaa cccgcggttc cagccagaag
    19741 gcacacggcg cgccgctttc gcctctgaat ttcttcgagt gtgaagagaa ggaaaaggcc
    19801 ggaaccgagc gcgagcggcc cgatcgctcc ggcccggtag cgtgaggtgg tctggacgag
    19861 gagacacgcc gcgagcggcg cgaggattcc gacgaatgcc agc
//
LOCUS       DAOMFY010000043        19878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_864_length_19878_cov_7.391616, whole genome shotgun sequence.
ACCESSION   DAOMFY010000043 DAOMFY010000000
VERSION     DAOMFY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_864_length_19878_cov_7.391616"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="PLB01_05670"
     CDS             <1..1853
                     /locus_tag="PLB01_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76822.1"
                     /translation="GAVLRAVGVLLAFAAGAAVTAGALALRARAPHQKTVVQGQILLS
                     SDDLMDGGGAAVVVSPDGGQFAYVTRGSRGRLYLRRLDRLVPVEVPESDGALSPFFSP
                     DGKWIAFFARGKLRKAPVSGGAPSTICSAPLPRGGAWVSDAMIVFSPQTSGGLSVVHA
                     SGGDPRPLTKADVAARERSHRWPAALPGGQAVLFATQLSGSDYDEGIIEAVELATGKR
                     TVVHKGGAFPRWAPSGHVLFVRKGTMYALPFEARGLAPAGRPVPVLERVMSSTGGEAP
                     SDGSAQLDVSASGFCAYRTGENETIYRLAVVDRKGTVVKKAGAPRGFMWARFAPDGNR
                     VAVVISSGAQSDVWVYDVGRDAFSKLTFEGDDQTVVWSPDGREIAYTSDRSLGLVTSP
                     GEAPATMRGVYLKRADGSGSPRLLFKTAGTPGVTGFSPDGRVLAFQVTRAGTQLDLGV
                     LRLRDGLADGEPSIVVADPANDGGGVFSPDGRWLAYDSTDGGFGHVFLRSAAGGDARW
                     QVSDQMGSNPRWARDGTLYFLRQDGSIVGTRVTAEGGAPVFGKEEALFKLESYDIGSR
                     ITNGWDVSPDGQRFVVLLREGTANPLEANHVTLVSNFADGLRSLAPAGGK"
     gene            1870..3072
                     /locus_tag="PLB01_05675"
     CDS             1870..3072
                     /locus_tag="PLB01_05675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ76823.1"
                     /translation="MLALLLKVVLALWALRAVNAALSLLLVPSLAPAPRRKGGAPFVS
                     IVVPARDEQRAIEAATRSKCLQDDPAFEVIVVDDRSSDGTRSILRTLESEFPGTLRVV
                     DGVEPPPEWLGKPHALDEGARAARATEPGDWLVFSDADVVFAPDLLARSLGHAEKNRL
                     DFLTLLPQMEARGFGEKLMVPTIPAAAFCYLPGWLMNVPSARDFGGGGGVFNLIRRDL
                     FDRIGGHEALKASVIDDIQLGRNARRGGGRTGIGFALDSIRLRMYHGFAEISKGLEKN
                     GFFGLLASLPLAVLSLAWVLFEGELPFAVLLGGAIERAMGRGNGATAALFTLEEISLS
                     LSILCAVLLVRGVLDVRLRLGLLSTLLHPVWVACLTWIFAKSTWVNGVLHRNEWRGRV
                     RDARSLRT"
     gene            3152..5824
                     /locus_tag="PLB01_05680"
     CDS             3152..5824
                     /locus_tag="PLB01_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76824.1"
                     /translation="MIPGTRLGPYEVIAALGKGGMGEVYRARDSRLGRDVAVKVLPEE
                     LFEGEERRKRFEREARLLAALNHPGIAAIYSFEEIPSSSSSSSSSSRHLLVMELVEGE
                     TLSARLEKGPLPLDQVLRCGAEMGRALDVAHRKGIVHRDLKPGNVMLTKSGVKLLDFG
                     LARSLEPDPIDASLSGLETQDKALTVEGAIVGTLPYMAPEQVQGRAADARTDIFALGA
                     TLYEMTTGRRAFSGSNQASLIASILTSEPAPIPSVRAAIPASLDRIVRTCLAKDPDDR
                     WQSAADVARELQWAAEEKGAGAPPPSARARGALRERLAWGIAALAVLLGTLSFLGRSP
                     QVPGELTRFKILPPPGQNLLGFALVSPEARRLLLLLRDDGGKNRLAIRSLDSLDVRIL
                     PATEDARGAFWSPDGLEVGFFSDGKLKRISADGGPARVVCDSEGAVWGAWSPSGTILF
                     AKRFGGPLYAVPAAGGTPVPATDLDAAAGEIHQNQACFLPDGRHFVYFWANADVSKKG
                     IRLGTLGSKETRPLFASDSNAVWADGYLIFARDDAVLAWRFDPKSLHLVGEPFPAFEN
                     VKWATGDNFMSLSAAGKRVAYVSWALQRHLVWVDRKGREIGVLGGIAGYADVRISPDG
                     RKVAVAIRSKAHGGNGDIWVLDAGRGTPERITTEPTDDFNPAWFPDGQRLAYVSDRFG
                     WYNLFARPTSGGPEKLLVRSDRDKTFPTILPDGRHILVNVVETPRYVRSVLSLDDPKD
                     VVRVGGDSPFSEEHPALSADGKWTAFDSLESGQREVYVQPLAGGPKRQVSVDGGQMPV
                     WSRNGRELFYAARNGTLMSVALRPDGGRLEAGEPQPLFPLQFDLSGELTWHLLPYGVT
                     PDGERFLVIRRAPGVEPDGVVVVSNWTAALKKTQ"
     gene            5821..7818
                     /locus_tag="PLB01_05685"
     CDS             5821..7818
                     /locus_tag="PLB01_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76825.1"
                     /translation="MTLAAGTKLGAYEIVGLLGKGGMGEVYRARDVRVGRAVALKVLP
                     EELFEGEERRGRFEREARMLASLNHPGIAALYSFEEVPSSSSSSIRHILVMELVEGDD
                     LAVRLASGPLSPGESLSVAKQVAEALEAAHATGIVHRDLKPANVKVTPGGRVKLLDFG
                     LAKAVQAAPAETQAPTATASPTLAGAVLGTPAYMSPEQARGDLVDARTDVWAFGCLIC
                     EMVTGRAPFHRRTIAETLAAILGEPPRLEEAAWRAVPSLKALAARCLERDPARRPSSG
                     AVLVEALAGDGHGAPRSWLPLSLAAAAAGALVVGAWFLWKQPPRPASAGAAAPAVSPV
                     GTSAAYALYEKALAAFRTNTKADNARAIALYDEALRVDPKYAPAWAGLAFACARYSQE
                     YFDADPALAGRAEEAAAKALEFGPYLAEAHLARVLVLSSQHQNFDCRRALPEARRALE
                     LSPNLDIAHFWLGVGYALHLGLDDEAQVALSRAAELNPKWAPPVAGLGWVRLHQGRYD
                     ESLALTRRAAEMSPGFSVNYVVIADALLRLGRDSEADAEIARSLEKEPRNTRATSLRA
                     VLAARRGALAEADGLIEKAEALYDDHHVQYHAALVAAVRGDAAAAVARLRKAVAGNFN
                     PYGWYATDPLLASVRGDATFRAFLENSKRQMEADRAAFRAP"
     gene            7866..9113
                     /locus_tag="PLB01_05690"
     CDS             7866..9113
                     /locus_tag="PLB01_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HQQ76826.1"
                     /translation="MERFTGLIGLAVVWGVALALSKNRKAIRWRTIGWAFALQMVFGA
                     LVLYWEPGKQGLESFSNGVSHAIGYADQGSSFLFGWLAGPMDALGEKTGLGFRGIIFA
                     FKVLPIIIFICSFFSILYYFGIIQIIVKGMAWVMQKTMRVSGAESLCVAANVFIGQTE
                     APVLIAPYIVTMTTSELFTMMVGGMAHVSGAVMLAYVLMGAPLKYLITASVMAAPGTF
                     LITKIMWPETENPVTMGTVKMQIEKHDANFIDAAATGASQGMTLVLNIAAMLIAFVAL
                     IAMINGFLGWAGGLFGYPTFSLQTVFGWVLGPLAYILGAPKQDAAVVGNLIANKTVIN
                     EFFAFSMFKDVVGTLSEKGKIIATFALCGFANFSSIGIQIGGIGGLAPTRKSDLARLG
                     FRALLAGSLVSFMTAAIAGILMS"
     gene            9125..10033
                     /locus_tag="PLB01_05695"
     CDS             9125..10033
                     /locus_tag="PLB01_05695"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQQ76827.1"
                     /translation="MDPRLSAAAEWLRERLPHPPLVGVILGSGLGDFADGVDDAVRIS
                     YGDIPGFPASAVVGHAGALVAGKVHGVPTVVLSGRVHFYEGHPMASVVFPARVLGLLG
                     CRTVIVTNAAGGIDTSFRSGNLMLIEDHINGFGTNPLVGANEEDLGPRFPDMSDAYRK
                     DLRAIALAVARRERIVLKKGVYVGVHGPSYETPAEIRAFRRWGAHAVGMSTVPEVIAL
                     NQMSVGVIGISCITNMAAGVLKRPLVHAEVLETTQRVKGEFVRLLTALVRALGEGRSG
                     ATEDALAPRKGLARVLRRKAKGKGRG"
     gene            10030..10434
                     /locus_tag="PLB01_05700"
     CDS             10030..10434
                     /locus_tag="PLB01_05700"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQQ76828.1"
                     /translation="MSARPMTADEEARLVAAATAVRENAAAAYSKFKVGAALLTEDGR
                     IFTGCNVESASYGLTVCAERNAVFKAISEGAKGFRAVAVVTDAEPPASPCGACRQVLW
                     DQCRDIDVVIATPRGIATRTRLSALLPMAFEF"
     gene            10479..11981
                     /locus_tag="PLB01_05705"
     CDS             10479..11981
                     /locus_tag="PLB01_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ76829.1"
                     /translation="MKRRREGKEEISLKVNRALVSFVLATAACLAAPPAVAADAPHAP
                     SKKSPSSSSVSSVDTLVTAAHVLTMDGTDRVFSPGAVAIKGGAIVAVGAPSDLLSRYL
                     SKQKISRPRAVVLPGLVNTHTHAAMNLLRGIADDLPLMDWLTKFIFPAEGRNVSPGFV
                     KAGTLLACAEMVRGGTTTFADMYYFESDVAAAVDQCGMRAVLGETWIDFPVPDHKDLP
                     ESITMTRAFLEKWKGHPRVMAAIAPHAPYTNAKEALLAARDLAREFQAPLLIHLSETR
                     DEQKQIADKHGTTPAKWLDSIGFLGPNVLAAHGVWLDPDDLRLLAERRVGLAHNPESN
                     MKLASGVAPVVAARKAGIAVGLGTDGVAGSNNDLDMWEAMDFAGKLAKVSTMDPTALP
                     AKDLLRMATIEGARAMKLEDRIGSLEPGKRADLVAVDLGKSRTSPVWDLYSTLVYSAK
                     ESDVTLTMVEGRVLWNGTRVTTLDEAKVIRDAEDWRKKIAASLAAPAAGK"
     gene            11978..13288
                     /locus_tag="PLB01_05710"
     CDS             11978..13288
                     /locus_tag="PLB01_05710"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HQQ76830.1"
                     /translation="MSLFVRTIIKKRDGQELGEGEIADFVGGVTSGSITDEQAAAFLM
                     AVCIRGMTTRETAALTFAMMRSGETWDLSRHGFVSDKHSTGGVGDKATLVLAPLLAAV
                     GVKAGMMSGRGLGHTGGTLDKLESIPGFRTALPKSDFDRLLDTVGCALIGQTDSIAPA
                     DRRLYALRDVTGTVESIPLITASILSKKLATGAHAVVFDVKTGNGAFMADPRDAEKLG
                     RALVDTTRATGRKASGYLTAMDRPIGEAVGNANEVEESIRMLAGEGPADLRENTLLLG
                     VDLLLAAGAETNPKDARRRLNAALADGRALEVFARLVAAQGGDATVCDDPERLPQPVS
                     RRDVPAPEGGIVTRIATREVGMLAIELGCGRSKREDVIDPASGFRVKKKPGDAVVAGE
                     PLLVVELGPTAKPRADFFERLAACWTIAPAGAPVESMPFVVGTL"
     gene            13285..14676
                     /locus_tag="PLB01_05715"
     CDS             13285..14676
                     /locus_tag="PLB01_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HQQ76831.1"
                     /translation="MKRAALLLACALAAPPVAGDEAPQESPAPEAPALKVPVAPKKDF
                     TGALLESQAFNVIPLAWNRFVLQKEMGMVSWETWKENLNRGFAYDPDKFLTNMLSHPQ
                     HGGLYYGAARANGFNLYESFLFTALGSLTWEYFGEKSRPSTNDLIISSLSGVSVGETS
                     YRLSDLIFDNTATGFSRFLHEFAGLATSPGRSFQRILSGDAWRVGPNPEGTKPESLRA
                     ELLGGWLGVRSERPGEPVKKDGRGTVGWDVRYGDPFEKDLGKPFSTFRLVAEFTTARE
                     FVTRVQAEGNLAGWRLDDGPGDRNGLAVTFGYNFLNVDLTYGSQTLGLELASRIPAGT
                     GWDFVPRLQLLSAYTAVQTSEDSVKSTYRNYDWGVGGGVGVGARLRQRGRDVLRVDYT
                     DVWSYTANGPTVWNEVRVGQAAAFAPISKAWAVGAEYTFQEHRNHFPQGDQRRTSQQV
                     RALVSWTAFAGKP"
     gene            14696..17353
                     /locus_tag="PLB01_05720"
     CDS             14696..17353
                     /locus_tag="PLB01_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ76832.1"
                     /translation="MSLQAGSRLGVYEILGPLGAGGMGEVYRAKDPRLAREVAIKILP
                     EEFFEGEERRQRFEREARLLAALNHPGIAAIYSFEEIPSSSSSASRHILVMELVEGAT
                     LRERMKAGEISPREAIDVAAQIARGLAAAHARGIVHRDLKPENVVVSKDGRVKILDFG
                     LAKLSPKADAADELSSAGTRSLLTEAGAVFGTVGYMSPEQVRGEPVDGRSDIFSFGTL
                     LYELLAGRHPFRAATPAETMTAILRHEAPPLADVTPALSQIVVRCLEKTPERRFHSAE
                     DLAFALESSSGSTLAPALAAGGAGTRGHGRVALAAACAAGLVLGASAALFWGRAPRAM
                     PAKAVKFTIQPPEGGSFFNPVESTAIAFSPDGSQLAYVAWDPKGGRRVLLRALSGFES
                     RSLAGTEGALSVFWSPDGLSLGFFAENKLKRIELAGGAPVSICEVAGGAGKAGTWGRD
                     GDILYASVQGDAIYRVAASGGTPSVVLKPDKAAGETRIVWPWFLPDGERFLFYARFEG
                     GGRVMLAEPGKPPRVVLAAASRAQYSDPGYLVFVKEGSLLAQRFDARRGALSGAPLSI
                     AERVRYFMSTGAGTFATSRGGSLAYEPQDDAHRLVWFDRSGRATGTIGMAAKVNSVRI
                     SRDRRPILFDRAQGDLGTFDVWSYDVERNVETRLTSSPESEFSALWLPGGKSFIFSAV
                     RGANPRLVRRDVATGQEEFVLPEGGFQVAGDISPDGKHIAYFERTGKGAFDLWEAGLE
                     GGDAPRRLADGTYLSSNTRFSPDGRYLALTSRESGQAEAYVMPNPGPGEKTRVSTRGA
                     TSPRWSPDGRELFYLSGDMHLTSVPVRTSPTLELGTPKALFRIPGRSPWLGFDVSPDG
                     TRFLAIVPESIADEQPINVVVNWPAAVGK"
     gene            complement(17357..17902)
                     /locus_tag="PLB01_05725"
     CDS             complement(17357..17902)
                     /locus_tag="PLB01_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76833.1"
                     /translation="MVQIDIPVAFALGSLHADAARAQIAAGGRDSVWRTLAQDLLFFG
                     VFASWLPVHLLVRHFGFETSHMWWHEDSVLAYPLFIPAFMTLYLATNVAGYAAGVALV
                     RAGRVTLNRLVIAACWLFAAAWVLGQSSRTLVLGTYREWAAGTAPPMSSDPGLVRLLV
                     VFGVVFTIALVLWYRRIRSEA"
     gene            complement(17916..>19878)
                     /locus_tag="PLB01_05730"
     CDS             complement(17916..>19878)
                     /locus_tag="PLB01_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ76834.1"
                     /translation="QAPPAPAPTPAPAATPAPEATYADTIVVTASKTAETVRDAPVAV
                     SVIPASEIGNAATESYADLLRAVPGINTAQTSARDVNLTPRLATGRNARATLALLDGR
                     TIYQDYFGMVLWDLLPINFDEVRQVEVVRGPDSAMWGANAMGGVVNIITKDPAEMLGV
                     SGKILGGTLDTREASALYAGQDGAASYKISGSYFTQDAWERPTTLPDGTPLPAYENKG
                     THQYRLDGRLDLDQGDGGKWKLGAGFASSDGAMLTGLGPYDAENLYQGHAAAEYRKAD
                     FHAAANVTLHDAKYVGFLSPDVVTMKNQAISAEAGDRHLFGEKNLVTWGATARYSHFD
                     LSLVPDVHQRTDLAAFAEDEIFLAETLRLRIGARYDWSDTYGGAFSPRTGLIFEPHKD
                     HTFRLSWNRAFIAPSLVENFLYFPSSAMAPLPTGPYVFPILATGNEGLDAETMNAFEI
                     GYTGTFARRVTVTVSAYYNVSKDRIFFVPVEYYTSSAPPPGWPLPPSALDQIPLPKTF
                     SYRNLGRIVDRGIEFSAEGRITRELSAFANYSLQANPDVSEQGAGAIPVNLPSRHRIN
                     AGLNAAAGPFFGSLSVNYTSAAYWTDVQPYVGWTAPYTLWSAAAGWTFSKNVAVAVRA
                     VNLSDARVQQHVFGDILRRRVTAELRFHF"
BASE COUNT         2935 a         6772 c         7043 g         3128 t
ORIGIN      
        1 gaggcgccgt gttgcgtgcc gtgggcgtgc tcctcgcctt cgcggccggc gcagcggtca
       61 cggcgggggc gctcgcgctc cgcgcccgcg cgccgcatca gaagacggtc gtccagggcc
      121 aaatcctcct ttcctccgac gacctgatgg atggcggcgg cgctgccgtc gtcgtgtcgc
      181 ccgacggcgg ccagttcgcg tacgtgaccc gtggctcccg cggccgcctc tacctcagga
      241 ggctggatcg cctcgtcccc gtcgaagtgc cggagtcgga cggcgccctg agcccgttct
      301 tctcgccgga cgggaagtgg atcgcgttct tcgcgcgcgg caagctgcgc aaggcgccgg
      361 tttcgggggg cgcgccttcc accatctgca gtgcgccgct tccccgcggc ggcgcatggg
      421 tctcggacgc catgatcgtt ttttcgccgc agacgagcgg cggcctctcg gttgttcacg
      481 cgtccggggg agacccacgg cccctgacga aggcggacgt cgccgcccgc gaacggtcgc
      541 accgttggcc cgcggccctt cccggcggac aggccgtcct cttcgcgacg cagctctccg
      601 gcagcgacta cgacgaggga atcatcgagg cggtcgagct cgcgacgggg aagaggaccg
      661 tcgtccacaa gggaggcgcg ttcccgcgct gggctccttc cggccacgtc cttttcgtgc
      721 ggaaagggac gatgtacgcg cttcccttcg aagcgcgcgg gctcgctccg gcggggagac
      781 ccgtgccggt cctcgagagg gtcatgtcgt ccacgggagg ggaggccccc tcggacggca
      841 gcgcgcagct cgacgtgtcg gcgtcggggt tctgcgcgta ccgcacgggc gagaacgaga
      901 cgatctacag gctggccgtc gtcgaccgga aggggacggt cgtaaagaag gcgggcgcgc
      961 cgcggggctt catgtgggct cggttcgcgc ccgacggaaa tcgcgtcgcg gtcgtgatca
     1021 gcagcggggc gcagagcgac gtctgggtct acgacgtcgg ccgcgacgcc ttctcgaagc
     1081 tcacgttcga aggcgacgac cagacggtcg tgtggagccc cgacggtcgg gagatcgcgt
     1141 acacgtccga ccggtcgctc gggctcgtca cgagtcccgg ggaggctccc gcgacgatgc
     1201 ggggcgtcta cctgaagcgc gccgacggct cggggtcgcc gcgcctcctc ttcaagacgg
     1261 cggggacccc tggcgtgacg gggttttcgc ccgacgggcg ggtgctcgcc ttccaggtga
     1321 cgagagccgg aacccagctc gatctcggcg tgctgcgcct ccgggacggt ctggccgatg
     1381 gagaaccctc gatcgtcgtg gccgaccccg cgaacgacgg agggggcgtc ttctccccgg
     1441 acggccgctg gctcgcgtac gactccaccg acggcggttt cgggcatgtc ttcctccgga
     1501 gcgccgcggg tggggacgcg cgttggcagg tctcggatca gatggggtcg aacccccgat
     1561 gggcgcggga cgggaccctc tacttcctcc gccaggacgg gtcgatcgtc gggacccgcg
     1621 tcaccgcgga gggaggcgcg cccgtcttcg ggaaggagga agcgctcttc aagctcgagt
     1681 cgtacgacat cggcagccgg atcaccaacg ggtgggacgt ttcgccggac ggacagcggt
     1741 tcgtcgtcct cctgcgcgag gggacggcga acccgctgga ggcgaaccac gtgaccctcg
     1801 tctccaattt cgcggatggc ctccggagcc tcgcgccggc aggcgggaag tgacgcggga
     1861 cagccgggcg tgctcgccct cctcctgaag gtcgtcctcg cgctctgggc tctccgtgcg
     1921 gtgaacgcgg ccctgtcgct gcttctcgtg ccgtctctcg cgcccgcgcc ccgccggaag
     1981 ggcggcgcgc cgttcgtctc gatcgtcgtc cccgcgcggg acgagcaacg cgcgatcgag
     2041 gccgccacgc ggagcaagtg cctgcaggac gaccccgcgt tcgaggtgat cgtcgttgac
     2101 gatcgctcgt ccgacgggac gcgctcgatc ctgcggaccc tcgagtccga gttcccgggg
     2161 acgctgcggg tcgtggacgg cgtcgagccg ccacccgagt ggctcgggaa gccccacgcg
     2221 ctcgacgaag gggcgcgagc cgcaagagcg accgagccgg gcgactggct cgtcttctcg
     2281 gacgcggacg tcgtcttcgc gcccgacctc ctggcccggt cgctcgggca cgccgagaag
     2341 aatcgcctcg acttcctgac gctccttccg cagatggagg cgcgcgggtt cggcgagaag
     2401 ctgatggtgc cgacgatccc tgccgccgcg ttctgctatc tgcccggctg gctcatgaac
     2461 gtcccatccg cgagggactt cggcggcggg ggaggcgtct tcaacctgat ccgccgggac
     2521 ctcttcgacc gcatcggcgg ccacgaggcg ctcaaggcgt cggtcatcga cgacatccag
     2581 ctcggccgca acgcgcggag gggaggcggc cgcaccggga tcggcttcgc gctcgactcg
     2641 atccgcctga ggatgtacca cggcttcgcg gagatctcga aggggctcga aaagaacggg
     2701 ttcttcgggc ttctcgccag cctgccgctg gccgtcctgt cgctcgcgtg ggtcctcttc
     2761 gaaggtgaat tgcccttcgc ggtcctcctg ggcggcgcca ttgagcgggc catggggcgg
     2821 ggcaacggcg cgaccgccgc cctattcact ctcgaagaga tctctctctc cctctctatt
     2881 ctctgtgcgg tcctcctcgt gcgcggagtt ctcgacgtgc gcctgcgcct cgggctgctg
     2941 tcgacgctgc tgcatccggt ctgggtcgcc tgcttgacgt ggatcttcgc gaagtcgacg
     3001 tgggtcaacg gagtcctgca ccggaacgag tggcggggga gagttcggga cgcgcggagt
     3061 ctgagaacat gagcggtccc ccgtccggga tcatggcgcg cgtaaacgca gctcgcggcc
     3121 ggcgacgcgg tggccggtat ccttgaagga cgtgatcccc ggcacccgcc tcggccccta
     3181 cgaggtcatt gccgcgctcg gcaagggcgg catgggggag gtctaccggg cgagggactc
     3241 gcggctgggg cgggacgtcg cggtcaaggt gctgccggaa gagctcttcg agggcgaaga
     3301 gaggagaaag cggttcgagc gggaggctcg tctgcttgcg gcgctcaatc atcccggaat
     3361 cgccgcgatc tactcattcg aagaaatccc ctcgtcctcc tcgtcctctt cctcttcatc
     3421 gcgccacctc ctggtcatgg agctcgtcga gggcgagacg ctgtcggcac gcctcgagaa
     3481 gggccctctg ccgctcgacc aggtcttgcg ctgcggcgcg gagatgggcc gggccctcga
     3541 cgtcgctcac cgtaagggga tcgtccaccg ggacctcaag cccggcaacg tgatgctgac
     3601 gaaatcgggc gtcaagctgc tcgacttcgg gctcgcccgg tcgctcgagc ccgacccgat
     3661 cgatgcctcg ctgtcgggcc tggagacgca ggacaaggct ttgacggtcg aaggcgcgat
     3721 cgtcggaacc ctgccgtaca tggcgcccga gcaggtgcag ggccgggccg cagacgcccg
     3781 gacggacatc ttcgccctcg gcgcgacgct ctacgagatg acgacgggcc ggagggcgtt
     3841 ctccggctcg aaccaggcga gcctcatcgc gtcgatcctg acgtccgagc cggcgccgat
     3901 tccttccgtg cgggcggcaa tcccggcgtc tctggaccgg atcgtgcgga cgtgtctcgc
     3961 gaaggatccc gacgaccgct ggcagagcgc ggcggacgtc gcccgggagc tccagtgggc
     4021 cgccgaggag aaaggcgcgg gcgcgccgcc accgtccgcg cgggcgcgag gagcattgcg
     4081 cgagcggctc gcatggggca tcgcggcgct ggccgtcctt ctcggcacgc tttcgttcct
     4141 cggccgcagc ccgcaggtgc ccggggagct cacgcgcttc aagatccttc cgccgccggg
     4201 ccagaacctc ctcggattcg ccctggtctc gcctgaggcg cggcgccttc tcctgttgct
     4261 ccgggacgac ggcggcaaga accggctcgc gatccgctcc ctcgacagcc tcgacgtccg
     4321 catcctcccg gcgacggagg acgcgcgcgg cgcgttctgg tcgcccgacg ggttggaggt
     4381 gggtttcttc tcggacggga agctcaagcg catctccgcg gacggcgggc ccgcgcgggt
     4441 ggtctgtgac agcgaaggcg cggtctgggg agcctggagt ccctcgggaa cgatcctctt
     4501 cgcgaaacgt ttcggcggcc cgctgtacgc cgttcccgcg gccggcggga cgcccgtgcc
     4561 ggccacggat ctcgacgccg cggcggggga gattcatcag aaccaggcct gcttcctgcc
     4621 cgacggccgg cacttcgtct atttctgggc gaacgcggac gtgtcgaaga aggggattcg
     4681 cctcgggact ctcggctcga aggagacccg tccgctgttc gcgtcggact cgaacgccgt
     4741 gtgggcggac ggatacctga tcttcgcgcg cgacgacgcg gtcctcgcgt ggcggttcga
     4801 cccgaagagt ctccatctgg tcggagagcc ctttccggca ttcgaaaacg tcaagtgggc
     4861 gaccggcgac aacttcatgt ccctgtcggc ggccgggaag cgcgtggcgt acgtctcctg
     4921 ggctctccag cggcacctcg tctgggtgga ccgcaagggg cgtgagatcg gggtgctcgg
     4981 gggaatcgcg gggtacgcgg acgtccggat ctcaccggac ggccggaagg tcgccgtcgc
     5041 gatccgcagc aaggcccacg gcggaaacgg cgacatctgg gtcctcgacg cgggccgcgg
     5101 aacgcccgaa cgcatcacca ccgaaccaac cgacgatttc aatccggcct ggttcccgga
     5161 cggtcagagg ctcgcctacg tctcggaccg gttcggctgg tacaacctct tcgcgcggcc
     5221 gacgtccggc gggcccgaga agcttctcgt ccggtccgac cgcgacaaga cgttcccgac
     5281 gatcctcccc gacgggcggc acatcctcgt gaacgtcgtc gagacgccgc gatacgtccg
     5341 gtccgtcctg tcgctcgacg atccgaagga cgtcgtccgg gtcggcggag attccccgtt
     5401 ttccgaggag cacccggcgc tctcggccga cgggaaatgg acggcgttcg actcgctcga
     5461 gtccgggcag cgggaggtct acgtccagcc gctagccggc ggcccgaagc ggcaggtctc
     5521 cgtcgacggg ggacagatgc cggtctggag ccgcaacggg cgcgagctct tctacgccgc
     5581 gcggaacggg acgctcatgt cggtggccct tcgccccgac ggcggccgcc tcgaagcggg
     5641 cgagccccag cctctcttcc cactgcagtt cgacctcagc ggggagctga cctggcacct
     5701 tcttccttac ggcgtgacgc ccgacggcga gcggttcctc gtgatccgcc gcgcgccggg
     5761 ggtcgagccg gacggcgtcg tcgtcgtctc gaactggacg gctgcgctga agaagacgca
     5821 atgaccctcg cggcgggcac gaaactcggc gcctacgaga tcgtgggcct gctcggcaag
     5881 ggagggatgg gagaggtcta ccgggcgagg gacgttcgcg ttggccgagc ggtcgccttg
     5941 aaggtgctgc cggaagagct ctttgaaggt gaagagagaa gagggcgctt cgagcgggag
     6001 gctcgcatgc tcgcgagcct gaaccacccc gggatcgccg ctctctactc gttcgaagaa
     6061 gttccctctt cctcttcctc ttcgatccgc cacatcctcg tgatggagtt ggtcgaaggc
     6121 gacgaccttg cggtgcgtct cgcctccggc cctctttcgc ccggagaatc tctgtctgtt
     6181 gcgaagcagg tcgccgaggc gctcgaagcg gcgcacgcga cggggatcgt ccaccgggat
     6241 ctgaagcccg cgaacgtcaa agtgactccc ggcggccgcg tgaagctcct ggacttcggc
     6301 ctcgcaaagg cggtacaggc ggcgcccgcc gaaacgcagg cgccgacggc gacggcttcg
     6361 cccacgcttg ccggcgcggt tctcgggacg ccggcgtaca tgagccccga gcaggcgcgc
     6421 ggcgacctcg tggacgcaag gaccgacgtc tgggcgttcg gttgcctgat ctgcgagatg
     6481 gtcacggggc gggcgccgtt ccatcgccgc accatcgccg agacgctggc ggcgatcctt
     6541 ggggagccgc cgcgtctcga ggaagccgcg tggagggcgg ttccgtcctt gaaggcgctc
     6601 gcggcccgct gcctcgagcg cgaccctgca cgccgaccct cctccggagc cgttctcgtc
     6661 gaggctctcg ccggcgacgg gcacggtgcg ccgcgctcgt ggcttccctt gtcgctcgcg
     6721 gccgcggcgg ccggcgccct tgtggtcggg gcctggtttc tctggaagca gcctccgcgt
     6781 cccgcctcgg cgggcgcggc ggcgcctgca gtgtccccgg tgggcacctc ggccgcgtat
     6841 gcactctacg agaaggctct cgcggccttc cggacgaaca cgaaggcgga caacgcgcgg
     6901 gcgatcgcgc tctacgacga ggcgctgcgc gtcgacccga agtacgctcc cgcctgggcg
     6961 ggcctcgcgt tcgcgtgcgc ccggtacagc caggagtact tcgacgcgga ccccgctctt
     7021 gccggccgcg ccgaagaggc ggccgcgaag gcgcttgaat tcggacccta cctcgccgag
     7081 gcgcacctgg cccgcgtgct ggtcctgtcg tcgcaacacc agaacttcga ctgtcgaagg
     7141 gcgctccccg aggcgcgccg ggccctcgag ctctcgccga atctcgacat cgcgcatttc
     7201 tggctgggtg tggggtatgc cctgcacctc gggctcgacg acgaggcgca ggtggctctc
     7261 tcccgcgcgg ccgagctcaa cccgaagtgg gcgccgcccg tcgcagggct cggctgggtc
     7321 cggctgcatc agggccgata cgacgaatcg ctcgctctca cccgcagagc ggccgagatg
     7381 tcgccgggct tcagcgtgaa ctacgtcgtc atcgcggacg ctctcctcag gctcgggagg
     7441 gactccgagg ccgacgcgga gatcgcccgc tcgctcgaaa aggagccgcg gaatacccgg
     7501 gcgacgagcc tccgggcggt cctcgccgcg cggcgcggcg ccctcgcgga ggccgacggc
     7561 ctcatcgaga aggccgaagc cctctacgac gaccatcacg tccaatacca cgcggccctc
     7621 gtcgccgccg tgcgcggaga cgcggccgcg gcggtcgcgc ggctgaggaa ggccgtcgcg
     7681 ggcaacttca acccttacgg ctggtacgcg accgacccac ttctcgcgtc cgtccggggc
     7741 gacgccacgt ttcgcgcgtt tctcgagaat tccaagagac agatggaggc cgatcgcgcc
     7801 gcattccgcg ctccctgagg gcgcgggacg gggtattctt ccgccttccc ggcacaggag
     7861 gactcgtgga gcgattcacc ggactgatcg gactcgcggt tgtgtggggc gtcgcactcg
     7921 cgttgtcgaa gaaccgcaag gccatccgct ggcgcacgat cggctgggcg ttcgccctcc
     7981 agatggtgtt cggcgcgctg gtcctctact gggagcccgg gaaacagggc ctcgagtcgt
     8041 tctcgaacgg cgtgtcgcac gcgatcgggt acgcggacca ggggtcgtcc ttcctgttcg
     8101 gctggctggc cggcccgatg gacgcgctgg gcgagaagac gggcctcggc ttccggggaa
     8161 tcatcttcgc gttcaaggtc ctgccgatca tcatcttcat ctgctcgttc ttctcgatcc
     8221 tgtactactt cgggatcatc cagatcatcg tcaagggcat ggcgtgggtc atgcagaaga
     8281 cgatgagggt ctccggcgcc gagtcgctgt gcgtcgcggc gaacgtgttc atcgggcaga
     8341 cggaagcccc cgtgctcatc gcgccgtaca tcgtcacgat gacgacctcc gagctcttca
     8401 cgatgatggt cggcggcatg gcgcacgtct cgggcgccgt catgctcgcg tacgtcctca
     8461 tgggcgcccc gctgaagtac ctcatcacgg cgtccgtcat ggcggcgccg ggcacgttcc
     8521 tcatcacgaa gatcatgtgg cccgagacgg agaaccccgt gacgatgggc accgtcaaga
     8581 tgcagatcga aaagcacgac gcgaacttca tcgacgcggc cgcgacaggc gcctcgcagg
     8641 gcatgacgct ggtcctgaac atcgcggcga tgctcatcgc gttcgtcgcc ctcatcgcga
     8701 tgatcaacgg gttcctcgga tgggccggcg gcctcttcgg gtatccgacg ttctccctcc
     8761 agacggtgtt cggctgggtc ctcgggccgc tcgcgtacat cctcggcgcg ccgaagcagg
     8821 acgccgcggt cgtcggcaac ctcatcgcga acaagaccgt catcaacgag ttcttcgcct
     8881 tctcgatgtt caaggacgtc gtcgggacgc tgtcggagaa gggcaagatc atcgcgacgt
     8941 tcgccctctg cggcttcgcg aacttctcgt ccatcggcat ccagatcggc gggatcggcg
     9001 gcctcgcgcc cacgcgcaag tccgacctcg cccggctcgg cttccgggcc ctcctcgccg
     9061 gctcgctcgt cagcttcatg accgccgcga tcgccggcat cctgatgtcc taggagaacg
     9121 acgcatggac cccaggcttt ctgccgccgc ggagtggctc cgcgaacgac tcccgcaccc
     9181 gccgctcgtc ggcgtcatcc tcggctcggg cctcggcgat ttcgccgacg gggtggacga
     9241 cgccgtccgg atctcctacg gcgacatccc gggcttcccg gcctcggcgg tcgtcgggca
     9301 cgccggcgcg ctcgtcgccg ggaaggtcca cggcgtgccg acggtcgtcc tctccggccg
     9361 cgtgcacttc tacgagggcc atccgatggc gtcggtggtg ttcccggcgc gcgtcctcgg
     9421 cctcctcggc tgccggacgg tgatcgtcac gaacgccgcc ggcggcatcg acacgtcctt
     9481 caggtccggg aacctcatgc tcatcgagga ccacatcaac gggttcggca cgaatccgct
     9541 cgtgggcgcg aacgaggagg acctcggtcc gcggtttccc gacatgtccg acgcctatcg
     9601 caaggacctc cgcgcgatcg cgctcgcggt cgcgaggagg gagcgcatcg tcctgaagaa
     9661 gggcgtctac gtcggcgttc acggcccgtc ctacgagacg ccggccgaga tccgcgcgtt
     9721 ccggcgctgg ggcgcgcacg ccgtcgggat gtccaccgtt cccgaggtga tcgcgctgaa
     9781 ccagatgagc gtcggcgtca tcgggatctc ctgcatcacg aacatggcgg ccggggtcct
     9841 caagcggccg ctcgtccacg ccgaggtcct ggagaccacc cagcgcgtca agggcgagtt
     9901 cgtgcggctc ctgaccgcgc tcgtgcgcgc cctcggcgag gggcggtccg gcgcgacgga
     9961 ggacgccctg gctccccgga aggggcttgc gcgagtgctg cgccggaagg cgaaggggaa
    10021 ggggcgcgga tgagcgcccg tcccatgacg gctgacgaag aagcccggct cgtggccgcc
    10081 gcgacggcgg tccgcgagaa tgcggcggct gcgtattcga agttcaaggt cggggcggcc
    10141 ctcctcaccg aggacggccg gatcttcacg ggctgcaacg tcgagtccgc gtcctacggc
    10201 ctcacggtct gcgccgagcg caacgccgtg ttcaaggcca tctccgaggg cgcgaaggga
    10261 ttccgggccg tcgccgtcgt gacggacgcc gaacctcccg cctcgccctg cggcgcgtgc
    10321 cgccaggtgc tttgggatca gtgccgcgac atcgacgtcg tgatcgcgac gccgcgcggc
    10381 atcgcgacgc gcacgcgcct ctcggcgctc ctgccgatgg cgttcgagtt ctagagaaag
    10441 agaaagatcg gaaagagaag aggaagattc ctttgaaggt gaagaggaga agagagggga
    10501 aagaggagat ttctttgaag gtgaataggg cgctcgtctc gttcgtcctc gcgaccgccg
    10561 cctgtctcgc ggcgccgccg gctgtcgctg ccgacgcccc tcacgcgcct tccaagaaat
    10621 ccccgtcttc ctcttcggtc tcctcggtcg acaccctcgt caccgccgcc cacgttctca
    10681 cgatggacgg cacggaccgt gtcttctccc cgggcgccgt ggcgatcaag ggcggggcga
    10741 tcgtcgcggt gggcgcgccg tccgatcttc tctcccgcta tctttcgaag cagaagatct
    10801 cccgtcctcg tgccgtcgtg cttcccgggc tcgtcaacac gcacacgcac gccgcgatga
    10861 atctcctgcg cggcatcgcg gacgacctgc cgctcatgga ctggctcacg aagttcatct
    10921 tcccggccga gggcaggaac gtctccccgg gcttcgtgaa ggccggaacg ctcctggcct
    10981 gcgccgagat ggtgcgcggc gggacgacga ccttcgcgga catgtactac ttcgagtcgg
    11041 acgtcgccgc agccgtggac cagtgcggga tgcgggccgt tctcggcgag acgtggatcg
    11101 acttcccggt gcccgaccac aaggacctcc ccgagtcgat cacgatgacg cgcgcgttcc
    11161 tcgagaagtg gaagggccat ccgcgcgtga tggcggccat cgccccgcac gccccgtaca
    11221 cgaacgcgaa ggaggcgctc ctcgcggcgc gcgacctcgc gcgcgagttc caggcgcctc
    11281 tcctgatcca cctctccgag acgagggacg agcagaagca gatcgccgac aagcacggga
    11341 cgacgcccgc gaagtggctc gactcgatcg ggttcctcgg gccgaacgtt ctcgcggcgc
    11401 acggcgtgtg gctggacccg gacgacctgc ggctcctcgc cgagcgccgg gtcggcctcg
    11461 cccacaaccc cgagtcgaac atgaagctcg cgtcgggcgt cgcgcccgtc gtcgcggcgc
    11521 ggaaggcggg gatcgcggtg gggctcggga cggacggcgt cgccggctcg aacaacgacc
    11581 tcgacatgtg ggaggcgatg gatttcgccg gaaagctcgc gaaggtctcg acgatggacc
    11641 cgacggccct gcccgcgaag gacctcctcc gcatggccac gatcgagggc gcgcgcgcga
    11701 tgaagctcga ggaccggatc ggatctctcg aacccggcaa gcgggcggac ctcgtcgcgg
    11761 tggacctcgg caagtcgcgc acgagccccg tgtgggacct ctactcgacg ctcgtctact
    11821 ccgcgaagga gagcgacgtc acgctgacga tggtcgaggg acgcgtgctc tggaacggga
    11881 cccgcgtgac gacgctggac gaggcgaagg tcatccggga cgccgaggac tggcggaaga
    11941 agatcgcggc ctctctcgcc gcgccggctg cagggaaatg agcctcttcg tccgcaccat
    12001 catcaagaag cgcgacggcc aggagcttgg cgagggggag atcgccgatt tcgtaggggg
    12061 cgtgacctcc gggtcgatca cggacgagca ggcggccgcg ttcctgatgg cggtctgcat
    12121 ccgcgggatg acgactcgtg agacggccgc gctcacgttc gccatgatgc gctcgggcga
    12181 gacgtgggac ctctcccggc acggcttcgt gtccgacaag cattccaccg gtggcgtcgg
    12241 cgacaaggcg acgctcgtcc tcgcgccgct cctcgcggcc gtgggcgtga aggccgggat
    12301 gatgtcgggc cgcggcctcg gccacacggg cggcacgctc gacaagctcg agtcgatccc
    12361 gggattcagg acggccctcc cgaagtcgga cttcgaccgc ctcctcgaca cggtcggctg
    12421 cgctctcatc ggccagacgg actcgattgc ccccgcggac cgccgcctct acgcgctgcg
    12481 cgacgtgacg ggaacggtcg aatcgatccc cctcatcacg gcgtcgatcc tctccaagaa
    12541 gctcgcgacg ggtgcgcacg ccgtggtctt cgacgtgaag acgggcaacg gcgccttcat
    12601 ggccgacccg agggacgccg agaagctcgg gcgcgccctc gtggacacga cgcgcgcgac
    12661 ggggcgcaag gcgtcgggat acctcacggc gatggaccga ccgatcggcg aggccgtcgg
    12721 gaacgcgaac gaggtcgagg agtcgatccg catgctcgcg ggcgaaggcc cggcggacct
    12781 gcgggagaac acgctgctgc tcggcgtcga cctcctgctc gcggcgggcg ccgagacgaa
    12841 cccgaaggac gcgcgacgga ggctgaatgc cgcgctcgcc gacgggcgcg cgctcgaagt
    12901 tttcgccagg ctcgtcgccg cgcagggcgg cgacgccacc gtctgcgacg atcccgagcg
    12961 cctgccgcag cccgtgtcgc ggcgcgacgt gccggcgccc gagggcggga tcgtcacgcg
    13021 gatcgcgacg cgcgaggtgg gaatgctcgc gatcgagctc ggctgcggcc gctcgaagag
    13081 ggaagacgtg atcgacccgg cctcgggctt ccgcgtgaag aagaagccgg gcgacgcggt
    13141 cgtggcgggc gagccgctcc tcgtcgtcga gctcggcccc acggcgaagc cgagggcgga
    13201 tttcttcgag aggctcgcgg cctgctggac gatcgcaccc gccggagcgc ccgtcgagtc
    13261 catgccgttc gtcgtcggga ctctttgaag cgcgcggcac tgctgctggc ctgtgcgctc
    13321 gccgccccgc ccgtggccgg agatgaggcg ccgcaggagt cgccggcgcc cgaggccccg
    13381 gccctgaagg ttccggtcgc tccgaagaag gacttcacgg gcgcgctcct cgagagccag
    13441 gcgttcaacg tcatcccgct cgcctggaac cggttcgtcc tccagaagga aatggggatg
    13501 gtctcctggg agacgtggaa ggaaaacctg aaccgcgggt tcgcgtacga ccccgacaag
    13561 ttcctgacga acatgttgtc gcacccgcag cacggcggcc tctactacgg ggcggcgcgc
    13621 gcgaacgggt tcaacctcta cgagtcgttc ctgttcacgg cgctcgggag cctgacgtgg
    13681 gaatacttcg gggagaagtc gcggccctcg acgaacgacc tcatcatctc gtcgctctcg
    13741 ggcgtgagcg tgggcgagac gtcctaccgg ctctccgacc tcatcttcga caacacggcc
    13801 acgggtttct cccgtttcct gcacgagttc gcggggctcg cgacgtctcc cggccggtcg
    13861 ttccagcgga tcctctcggg cgacgcatgg cgcgtgggcc cgaacccgga gggcacgaag
    13921 ccggagtcgc tgcgcgccga gctcctcgga ggctggctgg gcgtcaggtc cgaacgcccc
    13981 ggcgagccgg tgaagaagga cgggcgcgga accgtcgggt gggacgtccg gtacggcgat
    14041 ccgttcgaga aggacctcgg caagcccttt tcgacgttcc ggctcgtcgc agagttcacg
    14101 acggccaggg agttcgtcac gcgcgtgcag gcggagggaa acctcgcggg ctggaggctc
    14161 gacgacgggc cgggcgaccg gaacgggctc gcggtgacgt tcggctacaa cttcctcaac
    14221 gtcgatctca cgtacggcag ccagacgctc ggcctcgagc tcgcgtcgcg gattcccgcc
    14281 ggaaccggct gggacttcgt cccgcgcctg cagctcctgt cggcctacac ggccgtgcag
    14341 acctccgagg attcggtgaa gtccacgtac cgcaactacg actggggagt cgggggcggt
    14401 gtcggcgtcg gggcgcggct caggcagcgg gggcgcgacg tcctgcgggt cgactacacg
    14461 gacgtctgga gctacaccgc gaacggcccg acggtgtgga acgaagtgcg ggtcggccag
    14521 gctgccgcgt tcgcgccgat ctcgaaggcc tgggccgtcg gagccgagta cacgttccag
    14581 gagcaccgga accacttccc gcagggggac cagcggcgca cgagccagca ggtccgcgcg
    14641 ctcgtgtcgt ggacggcgtt cgccggcaag ccctgagcgg tagacttccg ggagattgag
    14701 tctccaggcc ggaagccgcc tcggcgttta cgaaatcctc ggtccgctcg gcgcgggggg
    14761 aatgggagag gtctaccggg caaaggaccc ccgcctcgcg agagaggtcg cgatcaagat
    14821 cctgccggaa gaattctttg aaggtgaaga gaggaggcag cggttcgaga gggaggcgcg
    14881 tctgctcgca gcgctcaacc atcccggaat cgccgccatc tactcattcg aagaaatccc
    14941 ctcttcgtct tcctctgcat cccgccacat cctcgtgatg gaactcgtcg agggcgcgac
    15001 gctccgcgag cggatgaagg ccggggagat ctccccgcgc gaggcgatcg acgtcgccgc
    15061 gcagatcgcg cgaggcctcg cggcggcgca cgcgaggggg atcgtccacc gcgacctgaa
    15121 gcccgagaac gtcgtcgtct cgaaggacgg ccgcgtgaag attctcgact tcggcctcgc
    15181 gaagctctcc ccgaaggccg acgcggccga cgagctctcg tccgccggga cccggtcgct
    15241 gctcaccgaa gcgggtgccg tgttcgggac cgtgggctac atgtcgccgg agcaggtgcg
    15301 cggggagccg gtcgacgggc gttcggacat cttcagcttc gggacccttc tctacgagct
    15361 gctcgcggga aggcacccgt tccgtgccgc gactccggcc gagacgatga ccgccatcct
    15421 gaggcacgag gcgccgccgc tcgcggacgt gacgccggcg ctctcgcaga tcgtcgtgcg
    15481 ctgcctcgag aagacgccgg aacggcgatt ccattccgcg gaagacctcg cgttcgcgct
    15541 ggaaagctcc tcgggctcga ccctggcgcc ggcccttgcc gccggcggcg ccggtacgcg
    15601 ggggcacggg cgtgtcgcac tcgcggcagc gtgcgccgcc gggctcgttc tcggcgcgtc
    15661 ggccgcgctt ttctggggac gcgcaccgcg cgccatgccc gcgaaggcgg tgaaattcac
    15721 gattcagcct cccgaaggcg gctccttctt caaccctgtc gagtcgacgg cgatcgcctt
    15781 ctcgccggat ggttctcagc ttgcctacgt cgcatgggat ccgaagggcg ggcggcgcgt
    15841 tctgcttcgg gcgctgtccg gctttgaatc gcgttctctg gcgggcaccg agggggccct
    15901 gtccgtcttc tggtcgccgg acggcctctc gctcggattc ttcgccgaaa acaagctgaa
    15961 gcgtatcgag ctcgcgggag gcgcaccggt ttcgatctgc gaggtcgccg ggggagcggg
    16021 caaggcgggg acgtggggac gggatgggga catcctctat gcgagcgttc agggagacgc
    16081 gatctaccgc gtcgcggcct ccggcgggac tccgagcgtc gtcctgaagc ccgacaaggc
    16141 cgcgggcgag acgcggatcg tctggccctg gttccttccg gacggcgaga gatttctctt
    16201 ctacgcacgc ttcgaaggcg ggggcagggt catgctggct gagcccggga agcctccgcg
    16261 cgtggtgctc gcggccgctt cgagggccca gtactccgat ccgggctatc tcgtgttcgt
    16321 gaaggaaggc agcctcctcg cccagcggtt cgatgcgcgg cgcggggcgc tgtcgggcgc
    16381 tccgctctcg atcgccgagc gcgttcggta cttcatgtcg accggcgccg ggacgtttgc
    16441 gacgtcccgc gggggctcgc tcgcctacga gccccaggac gatgcccatc gcctcgtctg
    16501 gttcgaccgc tccggccgcg cgaccgggac gatcggcatg gctgccaagg tcaacagcgt
    16561 gcggatctcc cgcgaccgcc ggccgatcct gttcgaccgc gcccagggtg acctcggcac
    16621 gttcgacgtc tggtcgtacg acgtcgagcg aaacgtcgag acccggctca cgtcctcacc
    16681 ggaaagcgag ttctctgccc tctggctgcc cggggggaag agcttcatct tctcggccgt
    16741 ccgcggggcg aacccccgcc tcgtgcgccg ggatgtcgcg acgggccagg aggaattcgt
    16801 gctcccggaa ggtggctttc aggtcgccgg agacatctcg ccggacggga agcacatcgc
    16861 gtacttcgag aggaccggga agggcgcgtt cgatctatgg gaagccgggc tcgagggcgg
    16921 cgatgcgccg cggagactcg cggacgggac gtacctgagc tcgaacaccc ggttctcgcc
    16981 cgacggccga tacctcgcgc tcacgtcgcg cgagtcgggt caggccgagg cgtacgtgat
    17041 gccgaatccg ggccccggcg agaagacgcg cgtatccacg cggggcgcga cctcgccgcg
    17101 ctggagtccg gacggacggg agctcttcta cctctcgggg gacatgcacc tgacgtcggt
    17161 tccggtccgg acgtccccga ctctcgaact gggcactccg aaagccctct tccggatccc
    17221 gggccgctcg ccgtggctgg gattcgacgt ctcgccggac ggcacgcgct tcctcgcgat
    17281 cgtgccggaa tccatcgcgg acgagcagcc gatcaacgtc gtcgtgaact ggcctgcagc
    17341 cgtcgggaag taggtctcag gcctcgctcc ggatccggcg gtaccagagg acgagggcga
    17401 tcgtgaagac gacgccgaag accacgagga gccggacgag gccgggatcc gacgacatcg
    17461 gaggcgccgt gcccgcggcc cactcgcggt aggtgccgag gacgagcgtg cgggagcttt
    17521 gcccgaggac ccacgccgcc gcgaacagcc agcaggccgc gatcacgagc cggttcaggg
    17581 tcacgcgtcc cgcgcggacc agcgcgacgc ccgcggcgta gcccgcgacg ttcgtcgcga
    17641 ggtagagcgt catgaacgcc ggaatgaaga gcggataggc gaggacggag tcctcgtgcc
    17701 accacatgtg cgacgtctcg aagccgaagt ggcgcacgag gaggtggacc ggcagccagc
    17761 tcgcgaaaac cccgaagaag agcaggtcct gcgcaagcgt ccgccagacg gaatcgcggc
    17821 cgcccgcggc gatctgggcg cgcgcggcgt cggcgtggag agagccgagg gcgaacgcga
    17881 ccgggatgtc gatctggacc atcggggcct ccgtcctaga agtggaagcg cagctcggcg
    17941 gtgacgcggc ggcgcaggat gtcgccgaag acgtgctgct ggacgcgcgc gtccgagagg
    18001 ttcacggcgc ggaccgctac cgcgacgttc ttcgagaacg tccagccggc cgccgcgctc
    18061 cagagcgtgt acggggcggt ccacccgacg tacggctgga cgtccgtcca gtaggcggcc
    18121 gaggtgtagt tcacggagag ggagccgaag aacgggcccg ccgcggcgtt caacccggcg
    18181 ttgatccggt gacgcgacgg gaggttcacg gggatcgcgc cggcgccctg ttcgctcacg
    18241 tccgggttcg cctgcagcga atagttcgcg aaagccgaga gctcacgcgt gatgcggccc
    18301 tccgcggaga actcgattcc gcggtcgacg atgcgcccga ggttccggta gctgaacgtc
    18361 ttcggaagcg ggatctggtc gagtgccgag ggcggaagcg gccagcccgg cggcggggcc
    18421 gacgacgtgt agtactcgac aggcacgaag aagatacggt ccttcgagac gttgtagtac
    18481 gcggagaccg tcaccgtcac gcgccgcgcg aacgttcccg tgtaaccgat ctcgaacgca
    18541 ttcatcgtct cggcgtcgag gccctcgttg cccgtggcga ggatcgggaa gacgtacggg
    18601 cccgtcggga gcggagccat ggcgctgctc gggaagtaca ggaagttctc gaccagggac
    18661 ggcgcgatga acgcgcggtt ccagctcagc cggaacgtgt ggtccttgtg gggctcgaag
    18721 atgaggccgg tgcgcgggga gaacgccccg ccgtaggtgt cggaccagtc gtaccgcgca
    18781 ccgatcctca ggcgcagggt ctcggcgagg aagatctcgt cctcggcgaa ggccgcgagg
    18841 tcggtgcgct ggtggacgtc gggcacgagg ctcaggtcga aatggctgta gcgcgcggtc
    18901 gcgccccacg tgacgaggtt cttctcaccg aagagatgcc ggtctcccgc ttcggccgag
    18961 atcgcctggt tcttcatcgt gacgacgtcg ggcgaaagga agcccacgta cttcgcgtcg
    19021 tggagcgtca cgttcgcggc cgcgtggaag tccgccttgc ggtactcggc cgcggcgtgg
    19081 ccctggtaga ggttctccgc gtcgtacggg ccgaggccgg tgagcatggc gccgtcgctg
    19141 gaggcgaacc cggctccgag cttccacttc ccgccgtcgc cctggtcgag gtcgaggcgc
    19201 ccgtcgagcc ggtactggtg cgtgcccttg ttctcgtacg ccggcagcgg cgttccgtcc
    19261 ggaagcgtcg tgggcctctc ccaggcgtcc tgggtgaagt acgagccgct gatcttgtac
    19321 gacgcggcgc cgtcctgtcc ggcgtagaga gcgctggcct cgcgcgtgtc gagcgtgccg
    19381 ccgaggatct tcccggacac gcccagcatc tcggcggggt cctttgtgat gatgttcacg
    19441 acgccgccca tcgcgttcgc gccccacatc gcggagtcgg gcccccggac gacctcgacc
    19501 tgcctgacct cgtcgaagtt gatcgggagg agatcccaca gcaccatccc gaaatagtcc
    19561 tggtagatcg tccgcccgtc gaggagcgcg agcgtcgcgc gggcgttgcg ccccgtcgcg
    19621 agacgcgggg tcaggttgac gtcgcgggcg gacgtctggg ccgtgttgat cccggggacc
    19681 gcgcggagca gatccgcgta gctctccgtg gcggcgttgc cgatctcgga agcgggaatc
    19741 acgctgaccg cgaccggcgc gtcccgcacg gtctccgccg tcttgctcgc cgtcacgacg
    19801 atcgtgtcgg cataggtggc ctcgggagcg ggcgtcgccg cgggcgccgg agtgggagcg
    19861 ggagccggcg gggcctgc
//
LOCUS       DAOMFY010000044        19868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_866_length_19868_cov_6.692576, whole genome shotgun sequence.
ACCESSION   DAOMFY010000044 DAOMFY010000000
VERSION     DAOMFY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19868
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_866_length_19868_cov_6.692576"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2525
                     /locus_tag="PLB01_05735"
     CDS             <1..2525
                     /locus_tag="PLB01_05735"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQQ76835.1"
                     /translation="CGIPVGAQALSLNATVVKPTGSGFLVLYPQGGTFPPVSTLNFLG
                     NDVIVNAAVVPISVSGGISMALGVSGGDVILDVNGYYAPTPSVTSLNSLTGDVTLAAG
                     TNVTITPTGNTLTIASTGGSGGPPTGAAGGSLSGTYPNPGIAAGAVGSAQIATGAVTD
                     VKVASGISYSKLTGVPGFVLRSGDTMTGSLTLSGGNLVLGVEPSSGAGGSIVKGGQPF
                     LHDTGQINTFLGLESGNGIAAGGSGIGNTGVGWGAFHDGSSGGGNTAIGNSALFHNTK
                     GDSNAAVGAGALSNNTTGGLNTAMGSFALQANTAGSRNTALGYGAGSLLTTGDYNIDL
                     YHPGVDGDYGVIRIGLAGYQTRAFLAGIRGVTTGATDALPVFVDSNGQLGTAGGTINA
                     TNFSGSLAGDVTGTQSATVVAQVGGQAAANVAAGAVAANAAASTNTASTIVKRDASGN
                     FAAGTITAALSGAASANVLKSGDTMTGTLTLDPGNISFSAATSTAATGSILKNGTRFL
                     HNYGTQNTFLGVGAGNFSMSLNPGEAFRNTGVGALALSGNATGSLNTAVGYFALLANT
                     SGSYNTAVGGGSLVNSRTGSRNTAVGGGTLSSSNGDDNTALGHDALYYALDAGRNTAV
                     GSAALRTTMGAQNTALGYQAGARAGAGTGYGGGAITFLENTTGNYNTFLGYGTGATAS
                     VSNCTAVGIDAYCDAGDQVRLGNFFVNSIGGKVGWSALSDARAKEDIRDLDAGLDLVL
                     RLRPVSYRLKGGNGRTDLGFLAQDVEALLGNDFGVVTAGGDPDRTLSLRYQDFIAPLV
                     KAVQQQQREIDEAKKESAALRAALEELKGELVGLRNSMREAR"
     gene            2522..4408
                     /locus_tag="PLB01_05740"
     CDS             2522..4408
                     /locus_tag="PLB01_05740"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQQ76836.1"
                     /translation="MNPAILAPFMVLFTLEAAIPEAAEIRNWTAPPYWTPPAAEAGER
                     PEASGEHGRTVQGRTALAGGPTALPFIALPPCRVIDTRGNAPLTGGFLPAATVRSYTV
                     TGVCGIPVGAQALSLNATVVKPTGSGFLVLYPQGGTFPPVSTLNFLGNDVIVNAAVVP
                     ISVSGGISMALGVSGGDVILDVNGYYAPTPSVTSVNGVTGDVTLATTKGLDVGLSGST
                     LTLEPDTAYLQQRVSGSCPAGSSIRAIATDGSVACQVDGPANAFVQGGNAFGATAVLG
                     TTDGQALELKASGSRVVRYEGSNVIAGNPENAVASGVQSATIAGGGLPAPPPNSGVLD
                     GPNRVTDNYGTVGGGDGNRAGDDAGLTGDAAWATVGGGLANTAAGMWSTVGGGGRNAA
                     GAAATVAGGYNSSATGYGAAIGGGTLNTASNYYSTVGGGGNNAASGWYATVPGGSNNA
                     AAGRNSFAAGFGAKANADGVVLFADNTIGDFVGSNPNVFAVAFTGGIGMWTSKSFATG
                     CYIAAGGGSWTCTSSREEKEDFVPVDGRAVLAKVAALPISEWRYRSEASGARHLGPMA
                     QDFRAAFGLGDRDTGIGLIDEAGIALAAIQGLNAKLAEREAEIEALKARLARLEALLV
                     RSSR"
     gene            4548..5288
                     /locus_tag="PLB01_05745"
     CDS             4548..5288
                     /locus_tag="PLB01_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQQ76837.1"
                     /translation="MKITGPSLPIAPGKIAPPLAPGVPSTGGADFLTTKLDVAVQWAQ
                     SNSLWPMPFGTACCAIEFMSVVSSHYDLSRFGAEVVRFSPRQSDLLIIAGTVVDKLAP
                     VLKKIYDQMSEPKWVISMGVCACTGGFYRAYHVVQGVDEIVPVDVYVAGCPPTPENLI
                     HGIMMLQAKIQSGELARHREAKTFVPIRVGEDPVTAIPAYSNAVMANLPGRHALPGMP
                     ADAEAEATPEGRREVDAGIAAARGRENP"
     gene            5285..7021
                     /locus_tag="PLB01_05750"
     CDS             5285..7021
                     /locus_tag="PLB01_05750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQQ76838.1"
                     /translation="MSATVAPPKGLEFLPVLEKKFGEKVSVREGLAADVPTLVTDAGS
                     LHETARFLRDDPAAAYDLFLDLASADLSRLPGPQAPKAGERFQANYILYSTKRNEHLR
                     LKVNLPEKDPSVPTLTDVWPAANWFEREAWDLMGIDFKGHPNQKRLLTHGGFVGHALR
                     KDYDAKQRWLCTEEDLLQTELAAQTDEPADMFETTTLNFGPSQPATHGTLRIVMRLDG
                     ETIVAAESECGYLHRCFEKMSEAHTYSQVFPYTDRLNYCSPMLNNNGYARAIESMLGV
                     TPPPRALAVRTILGEFSRVMDHLVCLAANLVDIGGLTNFWYAYQPREEIYALLEACSG
                     ARLTVSYGRVGGLAKDVPENFVPWCREIIKGTRARIDDVETLVTRNKIVWKRFRDTGV
                     VTKEQALAWGWTGPCLRATGVPFDLRKDQPYEWYGEVDFDVPIMTEGDNYARYLVRVE
                     EMKQSLRIIEQILDKLPGGPVRSDDYRVMLPPKEMVYNEMEALIYHFKLVFEGVKVPP
                     GEIYSATEGANGELGFSVVSDGSGFPYRLKVRPPCFPLFASFEKVIKGHPVADAVATL
                     GLLNIIAGELDR"
     gene            7021..7701
                     /locus_tag="PLB01_05755"
     CDS             7021..7701
                     /locus_tag="PLB01_05755"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HQQ76839.1"
                     /translation="MAAKVVTLKVPETTLAESIYLVEIAKGLARTFGHFWKNLTNMKG
                     LPTIQYPEMLRKYSGRFRGRHILKKREDDTLKCVACYMCETACPAECIHIEAGEHPVL
                     AYEKYPTVFEIDLLRCVYCGYCVDACPKDAIWMTRDYELSFFDRKSAVLGIPELTEKP
                     SDTAAGGPGYGYRPYEGESPMKKAGQFEPSGLPLLPALAERGIAINRMPPPPPPKPPA
                     PAPAAKAG"
     gene            8392..8691
                     /locus_tag="PLB01_05760"
     CDS             8392..8691
                     /locus_tag="PLB01_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HQQ76840.1"
                     /translation="MALGLRFEWDAAKNRANVRRHGVSFEEASSAFLDENGILIDDPG
                     HSIGEERFVLLGLTSSLRLLVICHCYRQRTDVVRLISARKADKGEREEYFGRFKR"
     gene            8688..8921
                     /locus_tag="PLB01_05765"
     CDS             8688..8921
                     /locus_tag="PLB01_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HQQ76841.1"
                     /translation="MRKHYDFSKGRPNPYARRLKRQITLRLDVATITYFRLLSEETAI
                     PYQSLINLYLRECAASKKHLRLAWERAPQSGVA"
     gene            8931..9359
                     /locus_tag="PLB01_05770"
     CDS             8931..9359
                     /locus_tag="PLB01_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76842.1"
                     /translation="MISWTLRVCALLGAGILLFFAVDRFRENQRFARSGMSAPAEPIA
                     GYTERTTTTKQVGIKIGESKLQTAWIFFTTQDRRRIRVHMGLPEDVLADFLAGKTVTV
                     EYLPDEPSLARFKGHASSPWSMALGGATLLGATVLFWRRM"
     gene            9398..9862
                     /locus_tag="PLB01_05775"
     CDS             9398..9862
                     /locus_tag="PLB01_05775"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HQQ76843.1"
                     /translation="MRRQGPFPTFFFAPAPARFALLLLAVALAAGRAAADDWPVLRAG
                     MWEFNRTIETPGTPGKPQTIQKKDCANPTEDMKKQNETLAKAGCKFSPVVRSGNTYTI
                     TSACTMQGVSSSGKSVLTFQGDGAYEIRVESDFGGKKTHEVLRAKRTGDCPR"
     gene            9889..10545
                     /locus_tag="PLB01_05780"
     CDS             9889..10545
                     /locus_tag="PLB01_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004653948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HQQ76844.1"
                     /translation="MERGSHDSLRLAIGAAALLLVLAASGVRPYDRLTWFLEVLPILV
                     VLPLLLATRGRYPLTSLLYVLIFLHALILIAGGHWTYARVPLGFRLEEVFALHRNPYD
                     KIGHFAQGFVPAIAAREILIRGGTVRGRKMLAFVVLSIVLAISASYELFEWGAALALG
                     KGADDFLGTQGDPWDTQSDMFFALVGGAAALLTLSRVHDRAIGALPRTPDATPGPEAT
                     "
     gene            10542..10970
                     /locus_tag="PLB01_05785"
     CDS             10542..10970
                     /locus_tag="PLB01_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76845.1"
                     /translation="MTTPLPVPPEASSPFVLEHPAGVRVSRELSERDLAAFGPPEPDA
                     RHLWEWYSLPPFRDETVTLSIGLGYKAGRLEVISLQNVDPRFDGAKDGWEARERGRAE
                     SLRLWLTAKGLAPGRYSWGEISVVFDEKSGFGSAGVRFAL"
     gene            10960..11706
                     /locus_tag="PLB01_05790"
     CDS             10960..11706
                     /locus_tag="PLB01_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76846.1"
                     /translation="MRFDDARPPNGRRPSPFAAWRGAAWAYVALFALAGFAFWPKYLS
                     QLGGPIDPYTHAHAIVATGWCLLLVAQPLLATRDVAAHRALGLATYALAPAFVLAAVL
                     LAHARFRAMDEATFRQEAPTLFLPLSAALLFSVSFFLAVRHRRETPLHARLMVLTGLP
                     MIDPVLGRVLFFFLPPLPHPLLYQAVTFGLTDLVVLLMFFVPPASARVRRAYAAPAAL
                     FVLAHAFWFTLAQGPAWVPFAAWFRSLPLP"
     gene            11861..12361
                     /locus_tag="PLB01_05795"
     CDS             11861..12361
                     /locus_tag="PLB01_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQQ76847.1"
                     /translation="MREVAALLCMLAALPGHVAHAQSASSASQSRAADLAAIEKLRQR
                     EIAATVARDPVALTSIWTDDAVRFAPGVPAEIGIAAIRESNERQTANKSVKVLRFEPE
                     TKDFTFLEDGWAVELRTFTGSVMVTPGEEPKQMRGAALVVLKRLPDGSWKVFRAMGAP
                     EPPTKR"
     gene            12374..12808
                     /locus_tag="PLB01_05800"
     CDS             12374..12808
                     /locus_tag="PLB01_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ76848.1"
                     /translation="MKRVTGISGIFLTSEAPRLLAVHPVLMSRDVSASIRFYRDLGFA
                     LAFQDRPVDPRYAGVARDGVELHLQWQDAAQWAHPIDRPTYRFLVEDVDALYASFRRA
                     GLLSEPPPGGSPWSAPGETPWGTREFHVLDPDGNGLQFYRPL"
     gene            12828..13526
                     /locus_tag="PLB01_05805"
     CDS             12828..13526
                     /locus_tag="PLB01_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQQ76849.1"
                     /translation="MKSAFLEFVRSVVCGGIGDVSRRLAASPALAVARAEVGAGREHA
                     PEFFFTEIAHYLYAGDTAVHMAAAAFRRPVAELLVAYGADCRARNRRGAQPLHYAADA
                     NRWEPAAQAETIAYLVSVGADANVFDKSGVTPLHRAVRTRSLPAVRALLDGGADPRTP
                     NKAGSTPLHLAVQTTGRGGSGTAHAREQQAGIVRLLLERGARPTDRDGRGRQASQASK
                     SEWVRTLLGGGAGA"
     gene            13523..14119
                     /locus_tag="PLB01_05810"
     CDS             13523..14119
                     /locus_tag="PLB01_05810"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQQ76850.1"
                     /translation="MIAPRPVTLEGHGVRLEPLEPRHEDGLAAAAADGKLWELWFTSV
                     PEPGQTERYVADALAGRQAGHMLPWTVRETGSGEIVGTTRYHDIVPALDRVEIGYTWY
                     AARWQKSHVNTACKLLLLSHAFETLGCAVVGLRTDNFNFASQRAIEALGAKKDGVLRH
                     HQARRDGTVRDSVMYSILAVEWPDVKRHLALRLERHAR"
     gene            14153..14671
                     /locus_tag="PLB01_05815"
     CDS             14153..14671
                     /locus_tag="PLB01_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ76851.1"
                     /translation="MTLEERLRAFDAARNALLDELGALEPAVLTAKPLAGKWSILEIV
                     EHLVLAERAVFKGMPDLSRLEAGERGLGNHARYLLVTFVLKAGIPVRAPSRRMLPQGG
                     RSLADLRRMWDENKAWLLSVVDHLGTEGLGRAVFEHPIAGPLTVEQSVRMVRVHLDGH
                     VKQIRALQRLLA"
     gene            14705..15235
                     /locus_tag="PLB01_05820"
     CDS             14705..15235
                     /locus_tag="PLB01_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ76852.1"
                     /translation="MTELVTDRLHLRQFRPGDFEEHAAICADPEVMRFIRAGALSRSD
                     SWWQLARYMGHWQLRGYGLWAVVERATDRLVGHLGFLDPEGGHGFEMGWALARGAWGK
                     GYALEGTRAALGYAFTTLQRNHVSCIIRHENARSIRLAERLGARLEREFEEGGVRLLD
                     YGIARDATQGPDPGRT"
     gene            15235..15519
                     /locus_tag="PLB01_05825"
     CDS             15235..15519
                     /locus_tag="PLB01_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HQQ76853.1"
                     /translation="MCRNIKTLFNFEPPATHEEIRASSLQFVRKLSGFNKPSQANEAA
                     FDRAVDQVEAAARLLIDSLVTNAEPRDREVEARKARERNAVRFGPRPRTR"
     gene            complement(15541..17274)
                     /locus_tag="PLB01_05830"
     CDS             complement(15541..17274)
                     /locus_tag="PLB01_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ76854.1"
                     /translation="MRRVLSLALALALPASSAQTPSGVSKSVVVSVTNLDVVVTDSNG
                     KPIPDLTAADFEVKQDGIVQPLTNFSFIRNGPAAAPAQAAEPAPEAAPAPAALPASEP
                     PQAARAHLIVFVDELHLTAVNKNRALKSLRKYLPTVVGPNVEVQFVTWDRALRIRGPF
                     TSDMAVLSSLMDSLGQETTIGEVTKREHDQLIREIDSAVMAGATAGPVLLQNAILNLR
                     SWCDQQAALVDTTLEALHASFAAVSGVEGRKVLFLLTEKFTPAPGRDIWDYLLYGTTR
                     AGSVTSISSSRRGGGNADVNDLSWTAWDRGPAFRALTPRANAAGISIVTIDASGLTTD
                     EMVSAENAASVGRMDDGIAQIDMQQAMGLIADETGGKTIVGRNDLARALKDLEADWTA
                     YYSLGYESPSAKPGVPRAIKVSVKRPGASVRSRKAVIERTPEEKVSDAVLTGVHIPRT
                     SNPLRASLTIGTPKPSGKMWLVPLEFRIPFDRLTLVPQGGRAKGAVLFTAVSATPDGR
                     ISPVTTERAPIDVPEQDLASLAGKAFTYSATLKVRAGPQVFSTGLTDEVSRLTSYVQP
                     HVLIGDKPGKR"
     gene            complement(17293..17700)
                     /locus_tag="PLB01_05835"
     CDS             complement(17293..17700)
                     /locus_tag="PLB01_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76855.1"
                     /translation="MRKGALVLLALFLAGAAAAEDAAPPPLRLPPVETFRLFPGAQPE
                     PRIELSGALEDRRLFHQPGDPDSQFTLGKPSPQGELRVLAVVDVNEIFLKAIAPDPLV
                     SSSDTRPRAAGSFLPVDGQGRPYQLRLGARLVW"
     gene            complement(17749..18525)
                     /locus_tag="PLB01_05840"
     CDS             complement(17749..18525)
                     /locus_tag="PLB01_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ76856.1"
                     /translation="MNPTFDLEGRVALVTGASRGIGAEIARTFAAANARVALHYGSSH
                     EAARAVADSIAAAGHARPLLVSADAADADARRRAMAEVVKGLGAPEILVNNAGFYKPN
                     PFEGADDAVFLTRWRATMAVNLEAAAHFSFLALPAMRERTWGRIVMIASRSAFRAETD
                     HPDYAVSKAGMVNLARCLARNEGPRGVTANSVCPGWVRTEAVREALDGPQAAEVLSQI
                     PLGRVASTADVANAVLYLVSPLGSYVNGIAIPLNGGSYLH"
     gene            complement(18522..19382)
                     /locus_tag="PLB01_05845"
     CDS             complement(18522..19382)
                     /locus_tag="PLB01_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76857.1"
                     /translation="MSEVREEAGVDIGATLAKAVVVPAGGRLEAFESFVCPSGDLPAL
                     AAFLSNRHGLRLAATGAGAHRLGSLVPSAAPPVLTDEFEAWGEGEKVLIGKADFTPTT
                     PHYLVSLGTGTSILRIGQEGRVSRAGGTGLGGGTLRGFGQLLLGQVDHDALTALAFQG
                     DRRRVDLLVRDLYPSGEIALQGDLTASNLGRIASRDSRDVANAITGLVGENIGLLAAA
                     LSKQEAVPFPLDVVYAGSTLRQNVALRDVLAFATSIGGARARFLPNGEFVGAVGALAR
                     ARMLAKGGLR"
     gene            complement(19379..>19868)
                     /locus_tag="PLB01_05850"
     CDS             complement(19379..>19868)
                     /locus_tag="PLB01_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQQ76858.1"
                     /translation="DFLRKVEEAAVRDRIAGANLILVSIGGNDLSHSLRSVVPGEAGG
                     DPTAAALATASLNLQRILSAVRAANGSSPIRILGLYDPFPEGFDRKMARATLLRWNVA
                     LEEASMGVENALVVPTADVFDGRPDRLSSDRFHPGAEGYTEIATRVFQTLRVPAREGR
                     TS"
BASE COUNT         3034 a         6812 c         7010 g         3012 t
ORIGIN      
        1 tctgcggcat ccctgtgggc gcgcaggcgc tgtcgctgaa cgcgacggtc gtcaagccga
       61 cgggctcggg cttcctcgtg ctctatccgc agggggggac gtttccgccg gtgtcgacgc
      121 tgaacttcct cggcaacgac gtgatcgtga acgcggcggt ggtgccgatc agcgtctcgg
      181 gaggaatctc gatggcactg ggggtctcgg gcggggacgt gatcctggac gtgaacggct
      241 actacgcgcc gacgccgagc gtcacgagcc tgaattcctt gacgggagac gtcacgctcg
      301 cggcggggac gaacgtgacg atcacgccga cgggcaacac gctgacgatc gcgtcgacgg
      361 gcggcagcgg cggcccgccg acgggcgcgg cgggaggcag cctctcgggc acctacccga
      421 acccggggat cgcggcaggc gcggtgggct ccgcacagat cgcgacgggc gccgtgaccg
      481 acgtcaaggt cgcgagcggg atctcgtact ccaagctgac cggcgtcccc ggcttcgtcc
      541 tcaggtccgg cgacacgatg acaggcagcc tcacgttgag cggcggcaac ctcgtgctcg
      601 gcgtcgagcc gtcgtcgggc gcgggcggca gcatcgtcaa gggcgggcag ccgttcctgc
      661 acgacacggg gcagatcaac accttcctcg ggctggaatc ggggaacggc atcgcggctg
      721 gcggttccgg aatcgggaac acaggcgtcg ggtggggcgc gtttcacgac ggctcgagcg
      781 gaggcgggaa cacggcgatc ggcaattccg cgctcttcca caacaccaag ggggacagca
      841 acgccgcggt gggcgcaggc gcgctgtcga acaacacgac cggcgggctg aacacggcga
      901 tgggctcttt cgcgctccag gccaacaccg cgggaagccg gaacacggcc ctgggatacg
      961 gggccgggtc tctcctgacg acgggtgact acaacatcga cctctaccac ccgggcgtcg
     1021 atggagatta cggggtgatt cgcatcggcc tcgccggata ccagacccgg gcgttcctcg
     1081 cggggattcg cggcgtcacc accggcgcga ccgacgccct tccggtcttc gtggactcga
     1141 acggacagct cgggacggcg ggcgggacga tcaatgcgac gaacttctcg ggctcgctcg
     1201 cgggcgatgt cacgggcacg caatcggcga ccgtggtcgc tcaggtcggc ggccaggccg
     1261 ccgcaaacgt ggcggcggga gccgtcgcgg cgaacgcggc ggcgagcacg aacacggcct
     1321 cgacgatcgt gaagcgcgac gcgtcgggga acttcgcggc cggtaccatc acggccgccc
     1381 tctccggcgc tgcgtccgcc aacgtcctga agtccggcga cacgatgacg gggacgctca
     1441 cgctggatcc gggcaacatc agcttctcgg ccgccacctc gaccgccgcg accgggagca
     1501 ttctcaagaa cggcactcgg ttcctgcaca actacggcac ccagaacacg ttcctcggcg
     1561 tcggagccgg gaacttctcc atgagcctga atccgggcga ggcctttcgg aacacgggag
     1621 tcggcgccct tgcgctgtcc ggcaacgcga ccgggtcgct caataccgcc gtcggctact
     1681 tcgctctgct tgccaacacg tcgggctcgt acaacacggc cgtcggcggc ggcagcctgg
     1741 tcaactcccg cacgggttcg aggaacaccg ccgtgggcgg tggcaccctg tcctcttcca
     1801 acggagacga caacacggcg ctcggtcacg acgcgcttta ctacgccctc gatgctggca
     1861 ggaacaccgc ggtgggctcc gcggctctga ggacgaccat gggcgcgcag aacaccgctc
     1921 tcggctacca ggccggcgca cgagccggcg ccgggacggg ctacggcggc ggcgccatca
     1981 cgttcctcga gaacacgacg ggcaattaca acaccttcct cgggtacggc accggcgcga
     2041 ccgcgtccgt ctcgaactgc acggccgtgg gcatcgacgc gtactgcgac gcgggcgacc
     2101 aggtgcgcct cggcaacttc ttcgtgaact ccatcggcgg caaggtcggc tggtcggcgc
     2161 tgtcggacgc gcgcgcgaag gaggacatcc gcgacctgga cgccgggctc gacctcgtcc
     2221 tgaggctccg gcccgtgtcc taccggctga aaggcgggaa tggacggacg gacctcggct
     2281 tcctcgccca ggacgtcgag gcgctgctcg ggaacgattt cggcgtcgtc acggcgggcg
     2341 gggatccgga tcgcacgctg tccctgcggt accaggactt catcgcaccg ctcgtgaagg
     2401 cggtgcagca gcagcagcgg gagatcgacg aggcgaagaa ggagtccgcc gcgctccggg
     2461 ccgcgctgga agaattgaag ggcgaactcg tcggtttgcg gaactcgatg agggaggcgc
     2521 gatgaatccc gccattctcg ctccgttcat ggtgctcttc acgctcgaag cggcgatccc
     2581 tgaggcggct gagatccgca actggacagc gccgccgtac tggacgccac cggcggccga
     2641 ggcgggcgag cggcccgagg cgtccggaga gcacggccgg acggtgcagg gccggacggc
     2701 gctcgcgggc ggcccgacgg cgttgccgtt catcgcgctg ccgccgtgcc gtgtcatcga
     2761 cacgcggggc aacgcgccct tgaccggcgg cttcctgccg gccgccacgg tgcgcagcta
     2821 cacggtgacg ggcgtctgcg gcatccctgt gggcgcgcag gcgctgtcgc tgaacgcgac
     2881 ggtcgtcaaa ccgacgggct cgggcttcct cgtgctctac ccgcaggggg ggacgtttcc
     2941 gccggtgtcg acgctgaact tcctgggcaa cgacgtgatc gtgaacgcgg cggtggtgcc
     3001 gatcagcgtc tcgggcggga tctcgatggc gctgggggtc tcgggcgggg acgtgatcct
     3061 cgacgtgaac ggctactacg cgccgacgcc gagcgtgacg agcgtgaacg gcgtcaccgg
     3121 cgacgtgacc ttggcgacga caaaaggcct ggacgtcggg ctgtccggaa gtacgctgac
     3181 gctcgagccc gacacggcgt acctccagca gcgggtgagc gggagctgtc ccgcggggtc
     3241 cagcatccgc gcgatcgcga ccgacgggag cgtcgcctgt caggtggacg gaccggcgaa
     3301 cgcgttcgtg cagggtggaa acgcgttcgg ggcgacggcg gtgctcggga ccaccgacgg
     3361 gcaggctctc gagctcaagg cgagcggcag ccgggtggtc cgctacgagg gttcgaacgt
     3421 gatcgcggga aaccccgaga acgccgtggc gtcgggggtc cagagcgcga cgatcgccgg
     3481 gggcggcttg ccggctccgc cgccgaattc cggggtgctc gatgggccga accgggtgac
     3541 cgacaactac ggcacggtcg gcggaggcga tgggaaccgc gcgggcgacg acgccggcct
     3601 gacgggtgac gcggcgtggg ccaccgtggg cggcggtctc gcgaacaccg cggccgggat
     3661 gtggagcacc gtcggagggg gaggccggaa cgccgcaggc gcggctgcga cagtcgcggg
     3721 gggatacaac agcagcgcga ctggctacgg ggccgctatc ggaggcggca cgttgaacac
     3781 ggcgagcaac tactactcga ccgtcggcgg cggcgggaac aacgctgcga gcgggtggta
     3841 cgcgacggtc ccgggtggca gcaacaacgc ggcggctggc cgcaacagct ttgcggccgg
     3901 cttcggcgcg aaggccaacg cggacggcgt cgtcctgttc gccgacaaca ccatcggcga
     3961 tttcgtcggg agcaacccga acgtcttcgc cgtcgccttc acgggcggta tcgggatgtg
     4021 gacgagcaag tccttcgcga cgggctgcta catcgcagcc ggcggcggct cgtggacctg
     4081 tacgagttcg cgcgaagaga aggaggactt cgtcccggtg gacgggcggg ccgtcctcgc
     4141 gaaggtggcg gcgctgccga tcagcgagtg gcggtaccgg agcgaagcct ccggggcgcg
     4201 gcacctcggt ccaatggcgc aggactttcg cgccgcgttc ggcctcggcg accgtgacac
     4261 gggaatcggg ctcatcgacg aggcaggcat cgccctcgcg gccatccaag gcctgaacgc
     4321 gaaactggcc gagcgggagg ccgagatcga ggcgctcaag gcccgtctcg cacggctcga
     4381 ggcgctcctc gtccggtcgt cccgctgagg cgggaagcgg ccgcgcctgc aattgaatcg
     4441 caggttcagc gaaggggcga aaacgcgcga tttcgccccc tcgcggcgcg actccgtgac
     4501 atagaatccg gctcgtgacg cttttcacaa gcgtctcgga gagccggatg aagatcactg
     4561 gacccagtct cccgatcgcg ccggggaaga tcgcgccccc gctcgcgccg ggcgtcccct
     4621 ccacgggcgg cgccgacttc ctcaccacga agctcgacgt cgccgtgcaa tgggcccagt
     4681 cgaactcgct ctggccgatg ccgttcggca cggcctgctg cgcgatcgag ttcatgagcg
     4741 tcgtctcgtc gcactacgac ctctctcgtt tcggcgccga ggtcgtgcgg ttttcgccgc
     4801 gccagtcgga cctcctcatc atcgcgggca cggtcgtcga caagctcgcg ccggtcctca
     4861 agaagatcta cgaccagatg tccgagccga agtgggtcat ctcgatgggc gtctgcgcgt
     4921 gcacgggcgg cttctaccgc gcgtaccacg tcgtgcaggg cgtcgacgag atcgtccccg
     4981 tggacgtcta cgtcgcgggc tgcccgccca ctccggagaa cctcatccac gggatcatga
     5041 tgctgcaggc gaagatccag tccggcgagc tcgcgcggca ccgcgaggcg aagacgttcg
     5101 tcccgatccg cgtcggcgag gaccccgtca cggcgattcc cgcgtactcg aacgcggtca
     5161 tggcgaatct ccccggccgc cacgccctcc ccgggatgcc cgccgacgcc gaggccgagg
     5221 cgacgcccga gggcaggcgc gaggtggacg ccgggatcgc ggcggcgcgc ggaagggaga
     5281 acccgtgagc gcgaccgtgg cgcccccgaa gggcctcgag ttcctgcccg ttctcgagaa
     5341 gaagttcggc gaaaaggtga gcgtccgcga gggcctcgcg gccgacgtcc cgacgctcgt
     5401 cacggacgcc gggtcgctgc acgagaccgc gcgcttcctt cgcgacgacc ctgccgccgc
     5461 gtacgacctc ttcctcgacc tcgcctcggc cgacctttcc cgcctgcccg ggccgcaggc
     5521 cccgaaggcg ggggagcgct tccaggcgaa ctacatcctc tactccacga agcgcaacga
     5581 gcacctgcgc ctcaaggtga acctccccga gaaggatccc tccgtcccca cgctcacgga
     5641 cgtctggccc gccgcgaact ggttcgagcg cgaggcgtgg gacctcatgg ggatcgactt
     5701 caagggccac ccgaaccaga agcgcctgct cacgcacggc ggcttcgtgg gccacgcgct
     5761 gcgcaaggac tacgacgcca agcagcgctg gctctgcacc gaggaggacc tcctccagac
     5821 cgagctcgcg gcccagacgg acgagcccgc ggacatgttc gagacgacga cgctgaactt
     5881 cggcccgtcg cagcccgcga cgcacggcac gctgcgcatc gtcatgcgcc tcgacggcga
     5941 gacgatcgtc gccgccgaga gcgagtgcgg gtacctgcac cgctgcttcg agaagatgtc
     6001 cgaagcgcac acgtactcgc aggtgttccc gtatacggac cgcctgaact actgctcgcc
     6061 gatgctgaac aacaacggct acgcgcgggc gatcgagtcc atgctcggcg tcacgccgcc
     6121 gccgcgcgct ctcgccgtgc gcacgatcct cggcgagttc agccgcgtca tggaccacct
     6181 cgtgtgcctc gcggcgaacc tcgtcgacat cggcggcctc acgaacttct ggtacgcgta
     6241 ccagccgcgc gaggagatct acgcgctcct cgaggcgtgc agcggcgcgc ggctcaccgt
     6301 gtcctacggg cgcgtcggcg gcctcgcgaa ggacgtcccc gagaacttcg tcccgtggtg
     6361 ccgcgagatc atcaagggca cgcgggcgcg catcgacgac gtcgagacgc tcgtcacgcg
     6421 caacaagatc gtctggaaac gcttccgcga caccggcgtc gtcacgaagg agcaggcgct
     6481 cgcgtggggc tggacggggc cgtgcctgag ggcgacggga gtgccgttcg acctgcgcaa
     6541 ggaccagccg tacgagtggt acggcgaggt cgacttcgac gtgccgatca tgacggaggg
     6601 cgacaactac gcccgctacc tcgtgcgcgt cgaggagatg aagcagtcgc tgcgcatcat
     6661 cgagcagatc ctcgacaagc tccccggcgg gcccgtccgg tcggacgact accgcgtgat
     6721 gctgccgccg aaggaaatgg tctacaacga gatggaagcc ctcatctacc acttcaagct
     6781 cgtcttcgaa ggggtgaagg tgccgccggg cgagatctac tccgcgaccg agggcgcgaa
     6841 cggcgagctc ggcttctccg tcgtctccga cgggagcggg ttcccgtacc gcctcaaggt
     6901 gcggccgccg tgcttcccgc tcttcgcgtc gttcgaaaaa gtcatcaagg ggcaccccgt
     6961 cgccgacgcc gtcgcgacgc tgggcctcct caacatcatc gccggggagc tggaccgctg
     7021 atggccgcca aagtcgtcac cctcaaggtg cccgagacga cgctcgccga gagcatctat
     7081 ctcgtcgaga tcgcgaaggg cctcgcccgg acgttcgggc acttctggaa gaacctcacg
     7141 aacatgaagg gcctgccgac gatccagtac ccggagatgc tccggaagta ctcgggccgc
     7201 ttccgcggcc ggcacatcct caagaagcgc gaggacgaca cgctgaagtg cgtcgcgtgc
     7261 tacatgtgcg agacggcctg cccggccgag tgcatccaca tcgaggcggg ggagcacccg
     7321 gtgctcgcgt acgagaagta cccgacggtc ttcgagatcg acctcctgcg ctgcgtctac
     7381 tgcggctact gcgtggacgc gtgcccgaag gacgcgatct ggatgacgcg cgactacgag
     7441 ctctccttct tcgaccgcaa gagcgccgtc ctcggcattc ccgagctgac ggagaagcct
     7501 tccgacacgg cggccggcgg cccgggttac ggctaccggc cgtacgaggg cgagagcccg
     7561 atgaagaagg cggggcagtt cgagccctcc ggcctgccgc tgctcccggc gctcgccgag
     7621 cgcggcatcg cgatcaaccg catgccgccc ccgccgccgc cgaagccgcc cgcgccggct
     7681 cccgcggcga aggcgggctg aggcctcaaa tgtctacaga tctagagatc gagaagtcac
     7741 tagatgtagg gaattcggcc gaaatgtctc taggggcgga gacatcggtt gccgtctaat
     7801 actagttagc tggccgtgaa tagcgcgcgc ggtggcctag tctcgttcgt gggtggcgca
     7861 gcattcgcgg ctgcattcat cacggccggt gccatcgtgg cggcgctgca gcttggccta
     7921 ctccaccagt cggaccctaa gttcggcggc cccgcggaca acttcgcgtt cggtctatac
     7981 ctggtcgggg ctggctgtct tttcgccaca ccagcattcg taatcgcaac cgcgcccttt
     8041 cgtgcgtggc gcgccctctc attgcaacgc gcgctcgcgt tcgcggtacc ggccggcgcc
     8101 ctcgccttcc tgcttcagtt tgtcggcctt ttgccgctcc tcttttcatg gctcgttccg
     8161 cgcaggccta gctctgtcgt cacatacgga acttacttct gcatctcagg cctcttaacc
     8221 ggcctcctcg tcgtgtctct cgcgcgccgc ctctgggcac cggtggcggc cagctaacaa
     8281 ctcattgcag cggactcggc gtcagtcgct cgcgctcctt ccttctcgcc ggtgaactcg
     8341 atatcgttag gtagtcttgc gcccgattga cgctgtaact acaatgtaat tatggcgttg
     8401 ggtctgcgtt tcgaatggga cgctgcgaaa aaccgagcga acgtacgtcg gcacggagtg
     8461 tcgttcgagg aagcctcgtc ggccttcctc gacgagaacg gcatcctcat cgacgatccg
     8521 ggtcactcca tcggagaaga gcgtttcgtc ctcctcgggc tcacatcgag cctgcggctc
     8581 ctcgtcatct gccattgcta tcggcaacgc accgacgtcg ttcgactcat ttcggcgcgg
     8641 aaggccgaca agggcgagcg agaggaatac ttcgggagat tcaaacgatg agaaagcact
     8701 acgacttctc gaagggccgg cccaatccct atgcaaggcg gctcaagcgc cagatcactc
     8761 tgcgcctcga cgtggccacg atcacgtact tcaggttgct ctcggaggag acggccatcc
     8821 cttaccagtc cctcatcaat ctctatcttc gggagtgcgc cgcgagcaag aagcaccttc
     8881 ggctcgcctg ggagcgggct ccgcaatccg gggttgcctg aaggcgcgcc atgatttcgt
     8941 ggacgcttcg agtctgtgcg ctgctgggcg ccggcatcct tctcttcttc gccgtggacc
     9001 gtttcaggga gaaccagcgc ttcgcccggt cgggcatgag cgcgccggcg gagcccatcg
     9061 cgggctacac ggaaaggacc acgaccacga agcaggtcgg gatcaagatc ggcgaatcga
     9121 agttgcagac tgcctggatc ttcttcacga cgcaggaccg ccgtcgcatt cgggtccaca
     9181 tgggccttcc cgaggacgtc ctcgccgact tcctcgccgg gaagaccgtc accgtcgagt
     9241 acctgccgga cgaaccgtcg ctcgcgaggt tcaagggcca cgcctcgagc ccctggagca
     9301 tggcgctcgg cggcgccacg ctgcttggcg ccacggtgct gttctggcgc cgcatgtgac
     9361 cgcgggaaat aatggcgtcc gcgaggaggc acccttcatg agacgacagg gcccgtttcc
     9421 gacgttcttc ttcgctcctg ctccggctcg attcgccctc cttcttctgg ccgtggccct
     9481 ggccgcgggc cgggccgcgg ccgacgactg gccggtgctg cgcgccggga tgtgggagtt
     9541 caaccgcacc atcgagacgc ccggcacgcc gggcaagcct cagacgatcc agaagaagga
     9601 ctgcgcgaac cctacggagg acatgaagaa gcagaacgag acgctcgcga aggcgggctg
     9661 caagttctcc ccggtcgtgc gttccggaaa cacgtacacg atcacctcgg cgtgcacgat
     9721 gcagggcgtc tcgtcgtccg ggaagagcgt gctcacgttc cagggcgacg gcgcgtacga
     9781 gatccgggtc gagtcggact tcggcggcaa gaagacgcac gaggtgctcc gggccaaacg
     9841 caccggcgac tgcccgcggt gacggccctg ggggatcccg cggtgcgcat ggagcgcggc
     9901 tcgcacgact ccctccggct cgcaatcggc gccgccgccc tgctcctcgt tctggcggcc
     9961 tccggcgtcc gcccgtacga ccgcctgacg tggttcctcg aggtcctgcc gatcctcgtc
    10021 gttctcccct tgctgctcgc gacccgcggc cgctacccgc tcacgagcct cctttacgtc
    10081 ctgatcttcc tgcacgccct gatcctgatc gcgggcggcc actggacgta cgcgcgggtt
    10141 ccgctcgggt tccgcctcga ggaggtgttc gcgctgcacc gcaacccgta cgacaagatc
    10201 ggccacttcg cgcagggctt cgtgccggcg atcgcggcgc gcgagatcct gatccgcggc
    10261 ggcactgtcc gcggccggaa gatgctcgcg ttcgtcgtcc tttcgatcgt cctcgcgatc
    10321 agcgcgagct acgagctctt cgagtggggc gcggcgctcg cgctcgggaa gggcgccgac
    10381 gacttcctcg gcacgcaggg cgacccgtgg gacacgcagt cggacatgtt cttcgccctc
    10441 gtgggcggcg ccgccgcgct cctgacgctg tctcgcgtcc acgaccgcgc aatcggcgcc
    10501 cttccccgga cgcccgacgc cacgccggga ccggaggcca cgtgaccacc cccctccccg
    10561 tcccgcccga ggcatcgtcg cccttcgtcc tcgagcatcc ggccggcgtc cgcgtctcga
    10621 gggagctgag cgaacgtgac ctggccgcgt tcggtccgcc cgagcccgac gcacggcatt
    10681 tgtgggagtg gtactcgctt ccgccgtttc gagacgagac ggtcactctt tcgatcgggc
    10741 tcggctacaa ggccggccga ctcgaggtga tcagcctgca gaacgtcgat ccccggttcg
    10801 acggtgcgaa ggacgggtgg gaagccaggg aacgagggcg cgcggagagt ctcaggctct
    10861 ggctgacggc gaagggcctt gctccgggcc ggtactcgtg gggagagatc tccgtcgtct
    10921 tcgacgaaaa gagcgggttc ggctccgccg gagtgcgttt tgcgctttga cgacgcgcgg
    10981 ccgccgaacg gtcggcggcc gtctccgttc gccgcgtggc gcggggccgc gtgggcctat
    11041 gtcgcccttt tcgctctcgc gggcttcgcg ttctggccga agtacctgtc gcagctcggc
    11101 ggcccgatcg atccgtacac gcacgcgcac gcgatcgtcg cgacgggatg gtgccttctc
    11161 ctcgtcgcgc agccccttct cgcaacgcgg gacgtcgcgg cgcatcgcgc gctcggactc
    11221 gcgacgtacg ctctcgcccc cgcgttcgtc ctcgccgccg tgcttctcgc gcacgcgcgg
    11281 ttccgcgcga tggacgaagc gaccttccgc caggaggcgc cgacgctctt cctgccgctc
    11341 tcggcggcgc tcctcttctc ggtctccttc ttcctcgccg tccggcaccg gcgggagacg
    11401 cccctccacg cgcgcctcat ggtcctgacg ggccttccga tgatcgaccc cgttctcggg
    11461 cgcgtcctgt tcttcttcct cccccctctg ccgcatccgc tcctctacca ggccgtcacg
    11521 tttggtctca cggacctcgt cgtcctcctg atgttcttcg tgccgcccgc gagcgcccgc
    11581 gtgcggcgcg cttacgcggc gccggccgcg ctcttcgtcc tcgcgcacgc tttctggttc
    11641 acgctcgccc aggggcccgc gtgggtcccg ttcgcggcct ggttccggag cctcccgctc
    11701 ccgtaagcgg cgccgtcccg cgagggggcg aggagttcgg cgcgccctcg cggccgcccg
    11761 gacccgcctc agacggcgcg ctccgcccgc ccgaacgcgc cgcacctgat atcttccggc
    11821 ggcgactcgt tctcaaaccg gggttgaccg aggaggggtc atgagagagg ttgccgctct
    11881 cctttgtatg ctggccgcgc ttccgggaca cgtcgcgcac gcacagtccg cgtcgtccgc
    11941 aagccagagc cgcgccgccg accttgccgc gatcgaaaag ctgcgacagc gggagatcgc
    12001 cgcgacggtg gcgcgagatc ccgtggcgtt gacgagcatc tggaccgacg atgccgttcg
    12061 gttcgctccg ggtgtgcccg cagagatcgg catcgcggcc atccgggaga gcaacgaacg
    12121 ccagacggcg aacaagagcg tgaaggtgct tcgcttcgaa ccggagacca aggacttcac
    12181 gttcctggag gatggctggg cggtcgagtt gcgcaccttc acgggatccg taatggttac
    12241 gccgggagag gagccgaagc agatgcgcgg cgccgcgctg gtcgtgctca agaggctccc
    12301 ggacggaagc tggaaggtgt ttcgcgcgat gggagccccc gaaccgccca cgaagcgctg
    12361 acggagggac cacatgaaac gcgtcaccgg catcagcggc atcttcttga ctagcgaagc
    12421 gccgaggctc ctggccgtcc acccggtcct gatgtcccgc gatgtgagcg cctcgattcg
    12481 cttctatcgg gacctcgggt tcgcgctggc cttccaggac cgccccgtcg atccccggta
    12541 tgccggcgtc gcccgcgacg gcgtggagct gcacctccaa tggcaggacg cggcgcaatg
    12601 ggcccatccg atcgatcgcc cgacctaccg cttcctcgtc gaggacgtcg acgcgctgta
    12661 cgcgtccttc cgccgcgccg gattgctgtc ggagccgcct ccgggcggca gcccgtggtc
    12721 ggcgccgggc gaaacgccct ggggaacgcg ggagtttcac gttctcgacc ccgacgggaa
    12781 cggactgcag ttctaccgcc ccctttaggc atgtgaagat ggccagggtg aaatcggcct
    12841 ttctcgaatt cgttcgctcc gtcgtctgcg gtgggatcgg cgacgtgtcg aggcgcctcg
    12901 cggcgagtcc cgcgctcgcg gtcgcgcgag ccgaggtcgg cgccggccgg gagcacgccc
    12961 cggagttctt cttcaccgag atcgcgcatt acctctacgc cggcgacacg gccgtccaca
    13021 tggccgcggc cgcgttccgg cgcccggtcg cggagcttct cgtcgcgtac ggggccgatt
    13081 gccgggcgag gaaccgccgc ggggcccagc cacttcacta cgcggcggac gccaaccgct
    13141 gggagcccgc ggcgcaggcg gagacgatcg cgtacctcgt gtccgtcggc gcagacgcca
    13201 acgtgttcga caagagcggg gtgacgcccc tgcaccgggc ggtgcgcacg cgttcgctgc
    13261 ccgcggtgcg ggcattgctg gacggcggcg ccgatccccg gacgccgaac aaggccggct
    13321 cgacgcctct gcacctcgcc gtccagacga ccggacgggg agggagcggc accgcacacg
    13381 cacgcgagca gcaggcgggc atcgtcaggc tcctgctgga gcgcggcgca cgcccgacgg
    13441 accgtgacgg gcgcgggcgg caggcgagcc aggcgtcgaa gagcgagtgg gtgcggaccc
    13501 tgctcggcgg aggggccggc gcatgatcgc gccccgcccc gtcacgctcg agggccacgg
    13561 cgtgaggctg gagccgctgg agccccggca cgaggacggc ctcgccgcgg cggcggccga
    13621 cggcaagttg tgggagctgt ggttcacgtc cgtgcccgag ccggggcaga cggagcgcta
    13681 cgtcgccgac gcgctcgccg gacggcaggc cggccacatg ctcccgtgga ccgtgcgcga
    13741 gaccggctcg ggggagatcg tcgggaccac gcgctaccac gacatcgttc ccgcgctgga
    13801 ccgcgtcgag atcggctaca cgtggtacgc ggcgcgctgg cagaagagcc acgtgaacac
    13861 ggcgtgcaag ctcctcctcc tctcgcacgc gttcgagacg ctcggctgcg ccgtcgtggg
    13921 cctccggacc gacaacttca acttcgcgag ccagcgcgcg atcgaagcgc tcggcgcgaa
    13981 gaaggacggg gtcctccgcc accaccaggc gcgccgggac ggcaccgtgc gcgacagcgt
    14041 gatgtacagc atcctcgccg tggaatggcc ggacgtgaaa cggcacctcg cgcttcgatt
    14101 ggaacggcac gcacgatgag tcctgtttcg cggcaatggt cgtaggggaa tcgtgactct
    14161 cgaagagcgc ctccgggcct tcgacgccgc tcgcaacgct ctcctcgacg agctgggggc
    14221 gctcgagccg gccgtgctca cggcgaagcc gctcgcggga aagtggtcga tcctcgagat
    14281 cgtcgagcat ctcgtcctcg ccgagcgcgc ggtgttcaag gggatgcccg acctgtcccg
    14341 gctcgaggcg ggcgagcgcg ggctcggaaa ccatgcccgc tacctgctcg tgacgttcgt
    14401 cctgaaagcc ggcatcccgg tgcgtgcgcc ttcacgcagg atgcttccgc aggggggcag
    14461 gagcctcgcc gacctgcgcc gcatgtggga cgagaacaag gcctggctcc tgtcggtcgt
    14521 cgaccacctc ggcaccgaag gcctcgggcg ggcggtgttc gagcacccaa tcgccgggcc
    14581 gctgaccgtg gagcagtccg tgcggatggt ccgggtccac ctcgacgggc acgtgaagca
    14641 gatccgggcg cttcagcggc tgctggcctg accgggcgtt ccgtgcgcgg tatcgttgcc
    14701 ggccatgacg gagctcgtca ccgatcgcct ccacctccgg caattccggc ccggtgactt
    14761 cgaggaacac gcggcgatct gcgccgaccc ggaggtcatg cgcttcatcc gtgcgggagc
    14821 gctgtcgcgc tccgactcgt ggtggcagct cgcgcgctac atgggccact ggcagctccg
    14881 cgggtacggc ctgtgggcgg tcgtcgagcg cgcgacggac cggctcgtcg gccacctggg
    14941 gttcctcgat cccgagggcg gacacggctt cgagatgggt tgggcgctgg cgcgcggagc
    15001 ctgggggaaa ggctacgcgc tcgaaggcac gcgcgcggcc ctgggttacg cgttcacgac
    15061 gctgcagaga aaccacgtct cctgcatcat ccgccacgag aacgcgcgct cgatccgcct
    15121 cgccgagcgg ctcggcgcgc gcctcgagcg cgagttcgag gaaggcggcg tgcgcctcct
    15181 cgactacgga atcgcgcgcg acgccacgca ggggccggac cccgggagga cgtgatgtgc
    15241 cggaacatca agacgctctt caatttcgag ccgccggcga cccacgagga gatccgggcg
    15301 tcttcgcttc agttcgtgcg gaagctgagc ggcttcaaca agccgtcgca ggcgaacgag
    15361 gcggcgttcg atcgcgccgt ggaccaggtc gaggcggcgg cgcggctcct catcgactca
    15421 ctcgtgacga acgccgagcc ccgggaccgc gaggtcgagg cgcggaaggc ccgggagaga
    15481 aacgcggtgc ggttcggccc gcgcccgcga acgcgctgat acgcggcttc cgcgcagcgg
    15541 tcagcgcttt ccgggcttgt cgccgatcag gacgtggggc tggacgtagc tcgtgaggcg
    15601 cgagacttcg tccgtgaggc ccgtcgagaa gacctgcggg ccggcgcgga ccttgagcgt
    15661 cgccgagtac gtgaacgcct ttcccgcgag gctcgcgaga tcctgctcgg gcacgtcgat
    15721 cggggcccgt tccgtcgtga cgggggagat gcgcccgtcg ggcgtcgccg agacggccgt
    15781 gaagaggacc gcgcccttcg cgcgcccgcc ctgcggcacg agcgtgagcc tgtcgaacgg
    15841 gatcctgaac tcgagcggga cgagccacat cttcccggag ggcttcgggg tcccgatcgt
    15901 gagcgacgcg cgcagcggat tgctcgtgcg cgggatgtgg accccggtga gaaccgcgtc
    15961 cgagaccttt tcctccggcg tgcgctcgat gacggccttg cgggagcgga cgcttgcgcc
    16021 ggggcgcttc acggacacct tgatcgcgcg gggcacgccg ggcttcgcgc tcgggctctc
    16081 gtacccgagc gagtagtacg cggtccagtc ggcctcgagg tccttgaggg cgcgggcgag
    16141 gtcgttgcgg cccacgatcg tcttcccgcc cgtctcgtcc gcaatcaggc ccatcgcctg
    16201 ctgcatgtcg atctgggcga tcccgtcgtc catccgtccg acggacgcgg cgttctcggc
    16261 ggaaaccatc tcgtccgtcg tgaggcccga cgcgtcgatc gtgacgatcg agatgccggc
    16321 cgcgttcgcg cggggggtga gggcacgaaa cgccgggccc ctgtcccagg ccgtccacga
    16381 gaggtcgttc acgtcggcgt tccctccgcc gcggcgcgac gaggagatgg aggtcaccga
    16441 gccggcgcgg gtggtcccgt agaggaggta gtcccagatg tcgcgccccg gcgccggggt
    16501 gaacttctcg gtcaggagga agagaacctt ccgcccctcg acgccggaca cggccgcgaa
    16561 cgaggcgtgc agggcttcga gcgtcgtgtc caccagggcg gcctgctggt cgcaccagga
    16621 gcggaggttc aggatcgcgt tctgcaggag cacgggcccg gcggtcgcgc ccgccatgac
    16681 ggcggagtcg atctcgcgaa tgagctggtc gtgctcgcgc ttggtgactt cgccgatcgt
    16741 cgtctcctgc ccgagggagt ccatgagcga ggagaggacc gccatgtcgc tcgtgaacgg
    16801 gccgcggatg cgcagcgcgc ggtcccacgt cacgaactgg acctctacgt tcgggccgac
    16861 gacggtcggc aggtacttgc gaagactctt gagggcgcgg ttcttgttga cggccgtcag
    16921 gtggagctcg tccacgaaga cgatgaggtg ggcccgagcg gcctgcgggg gttcggatgc
    16981 gggcagggcc gcgggggccg gcgcggcctc gggggcgggc tccgcggcct gggccggtgc
    17041 ggcggccgga ccgttgcgga tgaaggagaa gttcgtgagg ggctggacga tgccgtcctg
    17101 cttcacctcg aagtcggcgg cggtcaggtc cgggatcggc tttccgttcg agtccgtcac
    17161 gacgacgtcg aggttcgtga cggagacgac gaccgacttc gagactccgg acggcgtctg
    17221 ggccgacgag gcgggaaggg cgagcgcgag ggcgagcgag agaacacgtc tcatcgcctg
    17281 tcaggctacc acttaccaga cgaggcgggc gccgaggcga agctggtacg ggcgcccctg
    17341 gccgtccacg ggcaggaagc tcccggccgc ccgcgggcgc gtgtcgctcg aggacacgag
    17401 cggatccggc gcgatcgcct tcaggaagat ttcgttcacg tcgaccaccg cgaggacgcg
    17461 caattcgccc tgcggggacg gcttgccgag cgtgaactgc gagtccgggt ccccgggctg
    17521 atggaagagc cggcggtctt ccagggcgcc cgacagttcg atgcgtggct cgggctgggc
    17581 ccccgggaac agccggaacg tctcgacggg cggaaggcgg aggggcggcg gggcagcatc
    17641 ctcggcggcg gccgcgccgg cgaggaagag cgcgaggagg acgagggcgc ccttgcgcat
    17701 ggcctggtgg gaaattatcc gcccgatccg gccgcccggc gcaatgattc agtggaggta
    17761 cgagccgccg ttcaggggga tcgcgatccc gttcacgtac gagccgagcg gcgacacgag
    17821 gtagagaacc gcgttcgcga cgtcggccgt cgaggcgacg cgcccgagcg ggatctggga
    17881 gagaacctcg gcggcctgcg ggccgtcgag ggcctcgcgg acggcctccg tccggaccca
    17941 gccgggacag accgagttcg ccgtgacgcc gcgcggcccc tcgttccggg cgaggcagcg
    18001 tgcgaggttc accatgcccg ccttcgagac ggcgtagtcc gggtgatccg tctcggcccg
    18061 gaacgcggag cgcgaggcga tcatgacgat gcgcccccat gtccgttcgc gcatcgccgg
    18121 gagcgcgaga aacgagaagt gcgcggccgc ctcgaggttc acggccatcg tggcccgcca
    18181 gcgcgtcagg aacacggcgt cgtccgcccc ctcgaagggg ttcggcttgt agaagccggc
    18241 gttgttcacg aggatctcgg gtgcgccgag gcccttcacg acctcggcca tcgcgcggcg
    18301 gcgggcgtcc gcgtcggcgg cgtcggcgga gacgagcagc gggcgggcgt ggccggcggc
    18361 cgcgatcgag tcggcgacgg cgcgcgcggc ctcgtgcgag gagccgtagt ggagcgcgac
    18421 gcgcgcgttc gcggcggcga aggtgcgggc gatctcggcg ccgattccgc gggaggcgcc
    18481 ggtcacgagg gcgacgcggc cctcgaggtc gaacgtgggg ttcaccggag gcctcctttg
    18541 gcgagcatcc gcgcccgggc gagcgcgccg accgcgccca cgaactcgcc gttcgggagg
    18601 aaccgcgcgc gcgcgccgcc gatggacgtg gcgaaggcga ggacgtcgcg cagcgcgacg
    18661 ttctgacgca gcgtggagcc cgcgtagacg acgtcgagcg ggaagggaac ggcctcctgc
    18721 ttcgagagag cggccgcgag aaggccgatg ttttcgccga cgaggcccgt gatcgcgttc
    18781 gcgacgtcgc gcgagtcgcg cgacgcgatg cggccgaggt tcgaggccgt gaggtcgccc
    18841 tggagcgcga tctcgcccga ggggtagagg tcgcgcacga ggaggtccac ccggcgccgg
    18901 tcgccctgga acgcgagcgc cgtcagggcg tcgtggtcga cctggccgag cagcagctgg
    18961 ccgaatcccc tgagcgtgcc gcctccgagg cccgtgccgc cggcgcgcga gacgcgccct
    19021 tcctggccga tgcgcaggat cgacgtcccc gttccgaggc tgacgaggta gtgcggcgtc
    19081 gtgggcgtga agtcggcctt cccgatgagg accttttcgc cttcgcccca ggcctcgaac
    19141 tcgtcggtga ggacgggcgg cgcggccgac ggcacgaggc tgccgagccg gtgcgcgccc
    19201 gcgccggtgg ccgcgagacg caagccgtgg cgattcgaga ggaacgcggc gagcgccgga
    19261 aggtcgcccg aggggcagac gaaggactcg aaagcctcga gcctgccgcc cgccgggacg
    19321 acgaccgcct tcgcgagcgt cgcgccgatg tcgacgccgg cttcctcgcg gacctcgctc
    19381 atgacgtgcg cccctcccgc gcgggaacgc gcagcgtctg gaagacgcgc gtagcgatct
    19441 cggtgtagcc ctcggcgccc gggtggaaac ggtcggacga gaggcggtcc ggccggccgt
    19501 cgaagacgtc cgcggtgggg acgacgagcg cgttctcgac gcccatgctc gcctcctcca
    19561 gcgcgacgtt ccacctgagg agcgtcgcgc gagccatctt ccggtcgaaa ccttccggga
    19621 aggggtcgta gagaccgagg attcttatgg gggaagagcc gttcgctgcg cgaacggccg
    19681 agaggattct ttgaaggttc agagacgccg tcgccagggc cgcggccgtc gggtccccgc
    19741 ccgcctcgcc cgggaccacg gaccgaagag aatggctcaa gtcgttgccg ccgatcgaaa
    19801 cgaggatgag gttcgcgccc gcaatccgat cccgcaccgc cgcctcttcc acctttctga
    19861 gaaaatcc
//
LOCUS       DAOMFY010000045        19468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_890_length_19468_cov_7.499820, whole genome shotgun sequence.
ACCESSION   DAOMFY010000045 DAOMFY010000000
VERSION     DAOMFY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19468
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_890_length_19468_cov_7.499820"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLB01_05855"
     CDS             complement(<1..198)
                     /locus_tag="PLB01_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76859.1"
                     /translation="MSRAFSRRRFLASGSALAAAPLLPAFADGPAPSGATAPPLPPEL
                     PRETYRERQERALAAAKAAGLD"
     gene            complement(230..2131)
                     /locus_tag="PLB01_05860"
     CDS             complement(230..2131)
                     /locus_tag="PLB01_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQQ76860.1"
                     /translation="MTEPQTGPRSRLAVLSLAALGVVYGDIGTSPLYALRESLSPAEH
                     GLALNPENVLGVLSLIVWSLVLLVAVKYVWIVLRADNEGEGGILALMTLVSRCLRRDD
                     PRRHVVIVLGLFGAALLYGDGLITPAISVLSAAEGLKARSPALAPFVVPFAVVVLVVL
                     FSVQKKGTGRIGTFFGPVMLVWFGTLAALGAREILHAPGVLLAFDPMHAVRFFAHNGL
                     KGFLVLGSVVLVVTGGEAVYADMGHFGARPIRIAWFTIVLPALLLNYFGQGALLLREP
                     AALENPLFAMVPPWAVLPMVALATAATTIASQALISGVFSLTHQAVQLRFLPRLQVLH
                     TSATERGQIYLPAMNAALLVGCVFFVVSFGSSSRLAGAYGVAVTATMLLTTLLFTVLL
                     AAGWHAARWKIALFAAVFLTAEGAFFAANLAKLWHGGWIPLLLGAGIYSLMATWKQGQ
                     RVLLEALRRTSSAFLKPIEEHILDVRREKPPRLDHPAVYMERFHDLVPPALILNWQHN
                     RALHDPILLLTVETADVPRVPPSERRRVEDLGEGFFRVLLRWGFMEEPDVPEGLKGLV
                     LAGKPLDPDTISYVLGKDAIISTDRASGMARWRERLFSWMRRNEGRATAFYRIPPARA
                     IEFGIQVEI"
     gene            complement(2128..3309)
                     /gene="queA"
                     /locus_tag="PLB01_05865"
     CDS             complement(2128..3309)
                     /gene="queA"
                     /locus_tag="PLB01_05865"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQQ76861.1"
                     /translation="MDVADFDFELPPEAIAQRPALRGTARLMTLDRATGAFAHRSVAD
                     LPGLLAAGDVLVLNDTKVIPARIFGTDEKGRRTEFLLVERLGNEEGREEGISSKVEEG
                     ELWLCLAKPGRRVKTGRRFFFDGDWVAEAAAPGGMTGAAGAPPHAGPEGHSPSKKSAP
                     PPRAEGGLYAMRFTKHGSKGDSSLLSDLSVLGSAPLPPYIHRPGGLADAQDRLDYQTV
                     FAREPGAIAAPTAGLHFTESMLAETAARGVTIARLTLHVGLGTFKPVKSDRVEDHRMD
                     FERAFIPEDSAAAVNRAKDEGRRVVAVGTTSVRTLEASARLHGGRVAPGAFETDLFLV
                     PGAEFRVVDGLLTNFHLPRSTLLMLLSAFAGRERVLAAYSEAVRAGYRFFSYGDAMFV
                     S"
     gene            3335..4915
                     /locus_tag="PLB01_05870"
     CDS             3335..4915
                     /locus_tag="PLB01_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76862.1"
                     /translation="MRNRASAPVLLYALLAVLVVPVFPHFLSPNEVSRWAFAASLVER
                     RTPEISGVLPLLGPAFEDVSEKDGRVYSNKAPGAALVALPGALAARLIAGPPAPGSLR
                     LSLTLMRLLAATLPAILLALVLAGAAVRFGATPARARFAAAVLLFATPLFAYGLLLFS
                     HALVAACLFGAWAAFFAPGSGSRGRRDVLGGALLGLAVLSEYPAAVPAAVIVLCAAAV
                     SPRRLLAIAAGGAPPALVLAAYDTVCFGSPFALSSGFERAAAFRGLAASGLFGVGLPS
                     PSVLLRLLLDPSKGLFVFVPLLLLWPWAFPAARGRVPREGGLALALVPLSLLVLYAGY
                     PNWHGGFTVGPRYLVAALPFLVFPLAFREGGALEAGLAGASVLACVGTTLAFPFVPPG
                     FALPWGGTFGGDFYGKGLGAPNLFHLVPAMRGVAFVVPLLLVLAAAAALLSSHSKKRA
                     VAFCAGVTIAAAAALVAPRLWPLSPSLVLQRAYVADVYFGRRGALEDAIVATGSPQPR
                     LLARRERELLLPPSPWPF"
     gene            4961..5599
                     /gene="pyrR"
                     /locus_tag="PLB01_05875"
     CDS             4961..5599
                     /gene="pyrR"
                     /locus_tag="PLB01_05875"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQQ76863.1"
                     /translation="MGEAAGTYRKVRLLDAVGMARAISRMSHEVVEAEGGTEGFVLIG
                     IRTRGVPLAQRLASEIQRTEGVKVPVGLLDITLYRDDLTTVASSPVLKKTEIPFKVEG
                     RNVILCDDVLFTGRTVRAAMDAILDFGRPRKILLAVLVDRGRRELPIEAQYIGKKIAT
                     STTEIVSVTFKETDGEDDVWLLDREAAKPSPKAAPKAAPAKSPSARRRPRKK"
     gene            5613..6587
                     /locus_tag="PLB01_05880"
     CDS             5613..6587
                     /locus_tag="PLB01_05880"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQQ76864.1"
                     /translation="MTKRPDSAVPASPRRALSKKDLLDIDGITEDDIRLVLDTAESMR
                     EINQRAIKKVPTLRGKLVANLFFENSTRTRFSFEIAERRLSADSIALSAVGTSVAKGE
                     TLLDTGRNLMAMAPDAIVIRHSRSGAPHLLARNLPCAVINAGDGTHEHPTQALLDAFT
                     IRRAKGTLAGLRVAICGDVTHSRVARSNVALLTKMGAGVTLSGPRTLLPPEIERTGAR
                     ISTRIEEAIEGADVVMILRVQKERGGGLGFPSDREYYEQFGLTPERRKLAKKDAIVMH
                     PGPMNRGVEIDSAVADGPDSVILDQVENGVAVRMAVLYLLIGASGDDE"
     gene            6584..7891
                     /locus_tag="PLB01_05885"
     CDS             6584..7891
                     /locus_tag="PLB01_05885"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQQ76865.1"
                     /translation="MSGEAVLLRGGRVVDPSRNLDQTLDVLIRDGVVAKVDEKIPAKG
                     ADVVEAAGFVVAPGFVDLHTHLREPGFEYKETIASGTRAAAAGGFTTVCAMANTDPVP
                     DDAPTVAFVVRRARETGVVRVFPIGAVSKGLKGVELSEIGSMKAEGIVAISDDGKPIA
                     NGNLMRRALEYASMFGLPVVVHEEDAALVGPGVMNEGWASTRLGLAGWPNVGESAMVA
                     RDVQLAALTGARLHVAHVSTREGLEAIRAARKAGVPVTCEVTPHHLLLTDEDVARSGY
                     DTRFKMNPPLRSEADRNALVAALVEGTIDCVATDHAPHHEDEKNVEFADAPFGVTGLE
                     TALPALVDGLLVPRAITLLRLVEVLSTAPSRTFGLPYGTLAEGAPADVVLFSTTKSTP
                     VTPDGFQSRSKNSPWLARTLKGRVEKTWVGGREVYSRAPGPAR"
     gene            7888..8337
                     /locus_tag="PLB01_05890"
     CDS             7888..8337
                     /locus_tag="PLB01_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQQ76866.1"
                     /translation="MSSREDSGAYLKPVSSEVPVAAGKPKRIIIVEHEELFAEVWRAL
                     LVTRYGPAITVETYQDPLKALEACGPDVSLLLVDLELPLFGGRKMFELAKERGVAPRR
                     MVILSTRDANELHQLFPDGFCLAVINKHEPNQQNAFLMILDSIVKRH"
     gene            complement(8340..8825)
                     /locus_tag="PLB01_05895"
     CDS             complement(8340..8825)
                     /locus_tag="PLB01_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76867.1"
                     /translation="MRLTAKGSRTGALLFSPLALVLGCGRAPDAPKVPTATATSALNN
                     YTDVGITITRDATGKPVPDKDPLPLSKKELHRAHWKYCGAGLLNVHMTDESDDPFDGS
                     WERWSESECKHIRSPKIHPKAREKDQATATKPYKRYKYTISLLVDGQYIPNDPEIEID
                     R"
     gene            complement(8971..9927)
                     /locus_tag="PLB01_05900"
     CDS             complement(8971..9927)
                     /locus_tag="PLB01_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76868.1"
                     /translation="MTNTLAAVLFAAAAATPTPAPESHFAYDPKASIDAVTTGVETRG
                     DVVIQDLTYASPKGGRVPAYLVVPAGRGPFAAVLWGHWYWGNSPMKSRRQFLDEAVVL
                     ARSGVVSLLTDGPVARPGHVADRTPLNEKQFDDLVQQVVDMRRGADLLLARPEVDPRR
                     LAYVGHSYNATVGAILSGVDRRFKAFVLMAGALSDQVDLHTAEYRKYRDKVGPVRFDA
                     FMALHAWADPGPYVAKAAPAKVLLQYATQEDFLTPARAKEYAANVSEPKTLKIYDAPH
                     ALNAEARRDRLAFLSEVFAVKPPDPAAVANVPDLVQPPPPKP"
     gene            complement(9924..11201)
                     /locus_tag="PLB01_05905"
     CDS             complement(9924..11201)
                     /locus_tag="PLB01_05905"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQQ76869.1"
                     /translation="MSAARPRIVIVGGGFGGLYTARALRGAAADVTLVDRRNFHLFQP
                     LLYQVATGALSPANIAAPLRSIVRHQRNTRVLLEDVRGFDLARRAALTDAGEIPYDVL
                     VVASGASHAYFGHDEWERWAPGLKTIEDATEMRRRILLAFERAEITADAARRRALLTF
                     VIVGAGPTGAELAGAVAEIARRTLRDDFRTIDPADSRILLVEAGPRVLSSYAEDLSSK
                     AQAALASLGVDVRLGTAVTDVRDGAVVVVRDGRPETIEASTVLWAAGVAASPLGAALT
                     RSAGGETDRAGRVRVAPDLTLPGHPEIFVIGDLMTLPGADGRPLPGVAPVAMQQGRHV
                     ARSILARLEGRPGPGDFEYRDRGSMATLGRGRAVADLGFVRFAGYAAWLAWLFIHLMY
                     LVQFGNRVLILVQWAWSYLTWNRSARLITGRHP"
     gene            11235..12047
                     /locus_tag="PLB01_05910"
     CDS             11235..12047
                     /locus_tag="PLB01_05910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQQ76870.1"
                     /translation="MKSALDVATAAARVAGEVLVGHFGRLDPASVEEKAANDVVSIAD
                     RESEAVIRSAVLAAFPSDRFLGEETGLSGVDETAPSWIVDPLDGTANFVRGFPHWAVS
                     IARTRGGLLGEIEAGVVWDPVKNDLFTAELGSGAFRNGHRLRVPPRPDLRSAALATGF
                     PFRQAHKIDRYLAIFRELFLKVRSMRRAGSAALDLAYVGAGIFDGFFEFGLSPWDSAA
                     GALVVTEAGGRISDFDGGDTWRTRGNILAGSPGVHAALVAAMQEMGVQEKDL"
     gene            12044..12862
                     /locus_tag="PLB01_05915"
     CDS             12044..12862
                     /locus_tag="PLB01_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76871.1"
                     /translation="MSARGILLAFWAASAAMRAECQTPDWFDAFRGKAYLQGDASYVA
                     REKSAAPDSPPLFRARWVVVRYEGFSEKEREVWPYRNLLLVTMPGGAKYVVESAFGFV
                     DERHLEEDRPFHRVASARAVFEMWSAGGFEDPAAAADDPCGGSRQKVRAPGGSLDFIL
                     TDLSSRTVRTSLSEITAGTFDANERRDLVTLLKVAPDGAPSLPRDTFGLNARNALFSV
                     TVAFRGDVPPVAQRTVVLAPDPAPAGDLASWRALAHLPLDLPPFPAIVPENPIR"
     gene            12859..13491
                     /locus_tag="PLB01_05920"
     CDS             12859..13491
                     /locus_tag="PLB01_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76872.1"
                     /translation="MKTGGPLFLSKKVFFLLFLFSSVARAAEPPKVTLERIVDGRGNR
                     VCDVAKAELKAEGTRRSAWVLRGLFRSDLVLTLETAGRAEIHEIGFAGRPALRFTSPA
                     PGGRVTAASPASSFRYFEVDVATKTVRCNLGRLAEETDYELLRAAAEYFEQELEEDGL
                     ASEEAPLLTLRAVVKAPPRASPPIRKRVPWTEGGPEADELAAAARAAVSR"
     gene            complement(13493..14986)
                     /gene="glgA"
                     /locus_tag="PLB01_05925"
     CDS             complement(13493..14986)
                     /gene="glgA"
                     /locus_tag="PLB01_05925"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HQQ76873.1"
                     /translation="MRRRLRITHLAAECVPFAKTGGLGDVVGALPKALASGGNDVDVF
                     LPFHLEAAEWYRKRRAWPEEAMPSFDVSILGLPYRVGLLWDLLPGTTVPIYFVAHDPL
                     FHRRQIYAPNERGQDDGLWRFALFVRASIEALKRLGRKPRILHVHDWHPALGAMLAAW
                     SNWRDRWFDDVATVLTIHNLGYQGVYGRDQFPALGLPAEAWSSGGVEFGGNVNLLKGA
                     IVAADMITAVSPTYADEIRTPEGGFGLDAILRARGDRVVGILNGIDRGTWNPATDAHL
                     AARYAAYDLSGKAACRADLCRLAGFDPSDSGFLVGAIGRLTDQKGFDLLLESAPELIR
                     RGVRIVMLGSGEPAYEGSMRLLEARHPGHFKAFVGYDEALSHRIEAGADAFVMPSRFE
                     PCGLSQMYALAYGTPPIVRRTGGLADSVVPYDGFNLDRATGFAFDAPAPHALAAEILR
                     AQHVFFQPDRWRRIVENGMAQDFSWDRSAERYEAVYRRACEVRGLPW"
     gene            complement(14983..15822)
                     /locus_tag="PLB01_05930"
     CDS             complement(14983..15822)
                     /locus_tag="PLB01_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ76874.1"
                     /translation="MNARHVVALRGAAVALGAALALGCKPKPAEIRVTPAKLTFYGPG
                     RSQTVKFEVVDKKGRPLPGIPVTWTSDKPKIASVDANGLVRSLAPGRTTLTAAAQAVT
                     GNASVEVVDVASMAVSPNRMTLVGPPGTKMALLVEIKDAKGNAVNLKPKWMSGDPKIA
                     TVDANGIVTSAGEGRTTIIASLGNDQSSASDVRVLHRDIGTFEISPLTLILKIGETQR
                     INATVRDVSGMAIEDAALAWTSSEPKVAAVSNGAVVGLARGTAVITVATSSRTLTATA
                     IVN"
     gene            complement(15833..16462)
                     /gene="mtgA"
                     /locus_tag="PLB01_05935"
     CDS             complement(15833..16462)
                     /gene="mtgA"
                     /locus_tag="PLB01_05935"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQQ76875.1"
                     /translation="MRRLPLYLLSSLFFFSFFIFIPLPQIILLRWRDPSTTAFIRARQ
                     ERLRREGKSDSIDRRPVPLSQISPALVAAVVAAEDARFFEHHGIDWIAVAEARAYNRR
                     HAKDRRPRVHGASTITQQLAKNLWLSGERTWWRKAREASIALVLDACVPKKRILEVYL
                     SAIEWGERTWGCEAAARSLFQVPASRLNAQQAAAMAAMIPSPGRRGAIR"
     gene            complement(16541..17902)
                     /gene="lat"
                     /locus_tag="PLB01_05940"
     CDS             complement(16541..17902)
                     /gene="lat"
                     /locus_tag="PLB01_05940"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HQQ76876.1"
                     /translation="MASHVSPRLISNLGPKQVLDSLSSHMLVDGYHVVMDLKRSHGNF
                     IYDSLHEVEVLDFYSHFATCPIGYAHPKVTTPEFLDELAWAAVTKPANSDIYTEQMAL
                     FVETFARLAVPESHAKHMFFVEGGALANENALKAAFDWKVRRNFRKGHRREVGAKVLH
                     FEQAFHGRTGYTLSLTNTSDPRKTMYFPKFDWPRVVNPKLAFPLTPESLARTAAVEEL
                     AIRQIHQALAENPDDVAALIMEPIQGEGGDNHFRPEFLKRLRTICDENDLLFILDEVQ
                     TGMGLTGSMWAFQGLGVEPDMFTFGKKTQVCGFASNDRIMEEPENVFNVSSRINSTWG
                     GNLVDMVRCRKYLEIMVEENLVENAKTTGEFLLGKLVELGAEFPGKMTNIRGRGLFIA
                     FDLPDGKTRGDVLSCWLKKHHVMALTSGERAIRLRPPLTLTKDEAALGVQRLRAALTE
                     ILG"
     gene            complement(17971..19125)
                     /locus_tag="PLB01_05945"
     CDS             complement(17971..19125)
                     /locus_tag="PLB01_05945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HQQ76877.1"
                     /translation="MSRNARVISPKLLTRYMERHRLLDETSGTAPERFLVTLVNRAME
                     FVPSETCALLLDDPVSKEDDRSRNELTIIVLAGEGKDGVVGRKVRPVDGIAGFVYARG
                     ERTCVNEVGGAYAFSRETDGLLARDVLNVLAVPVVIENHVCGSLILLNRQSEPAFTPR
                     DERLMELFGDTLSLALQNLLDERRAREVARRDSLTSLYNDRYFHRKLQREIDRLAEAK
                     EGNLALLFLDCDHLKRVNDLHGHLAGSQVLREVSFILQRTLADTVSLACRYGGDEFTV
                     ILLEATRKRAMEIAESVRKAINDWVFLPMSIGPGEPALNLKNVISMSIGVATWRENVN
                     PALSAPEQKNELLKLADAALYMAKTRGKNCVVGAGDDEPGEDSLAVILKT"
     gene            complement(19175..>19468)
                     /locus_tag="PLB01_05950"
     CDS             complement(19175..>19468)
                     /locus_tag="PLB01_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76878.1"
                     /translation="RQALAPEPGETKERAAARVDRALREVLARRHGVSETLGASELLA
                     ALRDRGLPEARLADVKALLDDVDFLRFAPQLGEYDARIREVRERAARVLPLVA"
BASE COUNT         2828 a         6638 c         6983 g         3019 t
ORIGIN      
        1 atcgaggccg gccgccttcg cggccgcgag cgcgcgctcc tgccgttcgc ggtaggtctc
       61 gcgcgggagc tccgggggca gtggcggagc ggttgcgccc gacggcgccg gaccgtccgc
      121 gaaggccggg agcagcggcg ccgccgcgag ggcggagccg gaggcgagaa aacggcggcg
      181 ggagaaggcg cggctcaccg ggaggagact agccgatccg cgggcggcct cagatttcga
      241 cctggatccc gaactcgatc gcccgcgcgg gcgggatccg gtagaaggcc gtcgcgcggc
      301 cctcgttgcg gcgcatccag gagaagagcc gctcgcgcca gcgcgccatc ccgctcgcgc
      361 gatccgtcga gatgatggcg tccttgccga gcacgtagct gatcgtgtcg gggtcgaggg
      421 gctttccggc gaggacgagg cccttcaggc cctcgggcac gtccggctcc tccatgaacc
      481 cccacctgag gaggacccgg aagaaccctt ctcccaggtc ttcgacgcgc cgccgctcgg
      541 acggcgggac gcgcgggacg tcggcggttt cgacggtgag caggaggatc gggtcgtgca
      601 gcgcccggtt gtgctgccag ttgaggatca gcgccggcgg cacgaggtcg tggaagcgct
      661 ccatgtagac ggcggggtgg tcgaggcgcg gcggcttctc gcggcggacg tcgaggatgt
      721 gctcctcgat cggcttcagg aacgccgagg aggtccggcg cagcgcctcg aggaggacgc
      781 gctggccctg tttccaggtc gccatgagtg agtagatccc cgcgccgaga agaagcggga
      841 tccagcctcc gtgccacagc tttgcgaggt tcgcggcgaa gaacgccccc tccgccgtga
      901 ggaagacggc cgcgaagagg gcgatcttcc agcgggcggc gtgccagccc gccgccagaa
      961 ggacggtgaa gagcagcgtc gtgagcagca tcgtggcggt gacggcgaca ccgtatgcgc
     1021 ccgcgagccg gctcgacgag ccgaaggaga cgacgaagaa gacgcagccc acgagcagcg
     1081 ccgcgttcat cgcggggagg tagatctgcc cgcgttcggt cgccgacgtg tggagaacct
     1141 gaaggcgcgg gaggaagcgg agctggacgg cctggtgcgt cagggagaag acgccggaga
     1201 tgagcgcctg ggaggcgatc gtcgtcgcgg ccgtcgcgag cgcgaccatc gggaggaccg
     1261 cccacggcgg aaccatcgcg aagagggggt tctcgagggc ggccggttcg cgcaggagga
     1321 gcgcgccctg gccgaagtag ttgaggagga gcgcgggcag gacgatcgtg aaccacgcga
     1381 tgcggatcgg ccgtgcgccg aaatgcccca tgtcggcgta gacggcctcg ccgcccgtca
     1441 cgacgaggac gaccgatcca aggacgagga accccttcag cccgttgtgc gcgaagaaac
     1501 gcacggcgtg catcgggtcg aaggcgagga ggaccccggg cgcgtgcagg atctcccgcg
     1561 cgccgagggc cgcgagcgtc ccgaaccaga cgagcatgac gggcccgaag aacgtcccga
     1621 tccggcccgt ccccttcttc tggaccgaga agaggacgac gagaacgacg acggcgaacg
     1681 gcacgacgaa gggagcgagg gccgggctgc gcgccttcag gccctcggcc gcggagagga
     1741 ccgagatggc cggcgtgatg agcccgtcgc catagagaag cgccgcaccg aagaggccga
     1801 gaacgatgac gacgtgccgg cgcggatcgt ccctgcggag acagcggctg acgagcgtca
     1861 tgagcgccag gatgccgccc tctccctcgt tgtcggccct gaggacgatc cagacgtact
     1921 tgaccgcgac gaggaggacg agcgaccaga cgatgagcga aaggacgccg aggacgttct
     1981 ccgggttcag ggcgaggccg tgctcggccg ggctcagcga ctcgcgcagc gcgtagagcg
     2041 ggctcgttcc gatgtcaccg tacacgactc ccagagccgc gagggacagc acggccaggc
     2101 gcgagcgcgg gccggtttgc ggttcggtca cgagacgaac atagcatcgc cgtagctgaa
     2161 gaagcggtag ccggcccgga ccgcctccga ataggcggca agcacgcgct cccggcccgc
     2221 gaacgccgag aggagcatca ggagcgtgga gcgcgggagg tggaagttcg tgaggagccc
     2281 gtccacgacg cggaattccg cccctggaac gaggaagaga tccgtctcga acgcgccggg
     2341 cgcgacgcgc ccgccgtgga ggcgcgcgga ggcctcgagc gtccggacgc tcgtcgtgcc
     2401 gaccgcgacg acgcgccgtc cctcgtcctt cgcgcggttg acggcggccg cggagtcctc
     2461 ggggatgaac gcccgctcga agtccatccg gtggtcctcg acgcggtccg acttgacggg
     2521 cttgaacgtc ccgagcccga cgtggagcgt caggcgggcg atcgtgacgc cgcgcgcggc
     2581 agtctccgcg agcatggact ccgtgaaatg gagtccggcc gtcggcgctg cgatcgcgcc
     2641 gggctcgcgc gcgaagacgg tctggtagtc gaggcggtcc tgcgcgtcgg cgaggccgcc
     2701 ggggcggtgg atgtaggggg gaaggggagc ggagccaagg acggagagat cggagagaag
     2761 agaggagtcc cctttgctcc cgtgcttcgt gaaccgcatg gcgtagaggc caccttcggc
     2821 gcgcggggga ggggcgcttt tcttagaagg tgaatgtcct tccggtccgg cgtggggagg
     2881 cgccccggcc gcgcctgtca tgccgccggg ggccgctgct tcggccaccc aatcaccatc
     2941 gaagaaaaat ctcctccctg tcttcacccg cctccccggc ttggcgaggc agagccagag
     3001 ctcaccctct tcaaccttcg aagaaatccc ctcttctctt ccctcttcgt ttccgagtct
     3061 ttctaccagc agaaactccg ttcttcttcc cttttcgtcc gtgccgaaga tgcgcgcggg
     3121 aatgaccttc gtgtcgttga gcacgaggac gtcgcctgcg gcgagcaggc ccgggagatc
     3181 ggcgacggat cggtgggcga aggcgcccgt ggcccggtcg agcgtcatga gccgcgcggt
     3241 gccgcgcagg gcggggcgct gggcgatggc ctcgggcggg agctcgaagt cgaaatcggc
     3301 gacgtccacg gcgccgccga gggtacactt tcgcgtgcgg aatcgagcga gcgcgcccgt
     3361 tctcctctac gcgctgctcg ccgttctcgt ggttccggtc ttcccgcatt tcctctcgcc
     3421 aaacgaagtg tcgcgctggg cattcgcggc ttctctcgtc gaacgcagga cgcccgagat
     3481 ctcgggcgtc ctgccgctcc tcggcccggc attcgaggac gtttcggaga aggacggccg
     3541 cgtctactcg aacaaggcgc cgggggccgc gctcgtggcg ttgccgggcg ccctcgcggc
     3601 gcgcctcatc gccggtccgc ccgcgccggg atccctgcgt ctctcgctca cgctcatgcg
     3661 gctcctcgcg gcgacgctgc ccgcgatcct tctcgcgctc gtcctcgcgg gggcggcggt
     3721 gcgcttcggc gcgacgccgg cacgggcccg cttcgccgcg gcggtcctcc tctttgcgac
     3781 gccgctcttc gcgtacggac tgctcctctt ctcccacgcg ctcgtcgcgg cgtgcctgtt
     3841 cggcgcctgg gcggcgttct tcgccccggg ctccggctcg cgcgggcgcc gcgacgtcct
     3901 cgggggcgcg cttctcggcc tcgccgttct gtcggaatac ccggcggccg tcccggccgc
     3961 cgtgatcgtc ctgtgcgcgg ctgccgtgtc gccgaggcgg ctcctcgcga tcgccgccgg
     4021 aggagctccc cccgcgctgg tcctcgcggc ctacgacacg gtgtgcttcg gcagtccgtt
     4081 cgccctctcg tccgggttcg agcgcgcggc ggccttccgc ggcctcgccg cctcgggcct
     4141 gttcggcgtc ggcctgccgt ctccctccgt gctcctccgg ctcctcctcg acccgtcgaa
     4201 ggggttgttc gtcttcgtcc cgctcctcct cctctggccc tgggcgttcc cggcggcgcg
     4261 cggccgcgtc ccgcgggagg gcggtctcgc gctcgccctc gttccgctct cgctcctcgt
     4321 cctctacgcg ggctacccga actggcacgg cggcttcacc gtcgggccgc gctacctcgt
     4381 cgcggcgctg ccgttcctcg tcttcccgct cgcgttccgc gaggggggcg cgttggaggc
     4441 gggcctcgca ggggcgtccg tgctcgcgtg cgtcgggacg acgctcgcgt ttcccttcgt
     4501 ccctcccggc ttcgctctcc cctggggagg gacgttcgga ggagatttct atggaaaggg
     4561 gctcggggcg ccgaacctct tccacctcgt cccggcgatg cgcggggtgg cgttcgtcgt
     4621 gccccttctc ctcgtcctcg cggctgcggc ggccctcctg tcctctcatt cgaagaaacg
     4681 cgccgttgcg ttttgcgcgg gcgtgacgat cgcggccgcg gcggctctgg tggcgcctcg
     4741 tctctggcct ctttctccgt cgctcgttct tcagcgcgcg tacgtcgccg acgtgtactt
     4801 cggccgccgc ggcgcgctcg aggacgcgat cgtggcgacg ggctcgccgc agccgcgcct
     4861 cctggcgcgc cgcgagcgcg agctgctgct tccgccttct ccctggccgt tttgagcacc
     4921 gttgacggcg cgggagcgca ttggtagtgt ccgcccgccc atgggagagg ccgcggggac
     4981 ctaccgcaaa gtccgcctgc tcgacgccgt cgggatggcg cgtgcgattt cgcgcatgtc
     5041 ccacgaggtc gtcgaggccg aggggggcac cgaaggcttc gttctcatcg ggatccggac
     5101 gcgcggcgtc ccgctcgcgc agcgcctcgc ttccgagatc cagcgcaccg agggcgtgaa
     5161 ggtcccggtc ggtctcctcg acatcacgct ctaccgcgac gacctgacga ccgtcgcgtc
     5221 ctcgccggtt ctcaagaaga ccgagatccc gttcaaggtc gaggggcgga acgtgatcct
     5281 gtgcgacgac gtgctcttca cgggacgcac cgtgagggcc gcgatggacg ccatcctcga
     5341 cttcgggcgc ccgcgcaaga tcctcctcgc cgtcctcgtg gaccgtggcc gccgcgagct
     5401 gccgatcgag gcccagtaca tcgggaagaa gatcgcgacg tccacgaccg agatcgtctc
     5461 cgtgaccttc aaggagaccg acggcgagga cgacgtgtgg ctcctcgacc gcgaggccgc
     5521 aaagccctcg ccgaaggccg ccccgaaggc cgcgcccgcg aagtcgccct cggcgcgccg
     5581 gcgcccgagg aagaagtagg agccccggcg gcgtgacgaa gcgtcccgat tccgccgtcc
     5641 ccgcatcgcc ccgtcgcgcc ctttcgaaga aggacctcct cgacatcgac gggatcaccg
     5701 aggacgacat ccgcctcgtc ctcgacaccg ccgagtccat gcgggagatc aaccagcggg
     5761 cgatcaagaa ggtgccgacg ctgcgcggga agctcgttgc gaacctcttt ttcgagaact
     5821 ccacgcggac gcggttctcc ttcgagatcg ccgagcggcg cctctcggcc gactcgatcg
     5881 cgctctcggc cgtcgggacg tccgtcgcca agggtgagac gctcctcgac accgggcgca
     5941 acctcatggc gatggctccg gacgccatcg tgatccgcca ttcgcgctcc ggggcgccgc
     6001 atctcctggc acgcaacctc ccatgtgccg tcatcaacgc cggtgacggg acgcacgaac
     6061 atcccacaca ggcgctgctc gacgccttca cgatccgccg cgcgaagggg acgctcgcgg
     6121 gcctgcgcgt cgcgatctgc ggagacgtga cgcacagccg tgtcgcgcgg tcgaacgtcg
     6181 cgctcctgac gaagatggga gcgggcgtca cgctctccgg cccgcggaca ctcctgcctc
     6241 ccgagatcga gcgcacgggc gcccggatct ccacccgcat cgaggaggcg atcgagggag
     6301 ccgacgtcgt gatgatcctg cgcgtccaga aggagcgcgg cggcgggctc gggttcccct
     6361 cggaccgcga gtactacgag cagttcggcc tgacgccgga gcgccgcaag ctggccaaga
     6421 aggacgcgat cgtgatgcac cccggcccca tgaaccgcgg cgtggagatc gactcggccg
     6481 tcgcggacgg acccgattcc gtgattctcg accaggtcga gaacggcgtc gccgtgcgca
     6541 tggcggtcct ctatctcctg atcggcgcga gcggagacga cgaatgagcg gtgaagcggt
     6601 cctcctgcgc ggcgggcgcg tcgtcgatcc ctcgcgcaac ctcgaccaga cgctcgacgt
     6661 cctgatccgg gacggcgtcg tcgccaaggt ggacgagaag attcccgcca agggcgcgga
     6721 cgtcgtcgag gcggcggggt tcgtcgtcgc tcccggcttc gtggacctcc acacgcacct
     6781 ccgggagccc ggcttcgagt acaaggagac gatcgcgagc ggcacgcggg ccgcggcggc
     6841 gggcggcttc acgaccgtgt gcgcgatggc gaacaccgat cccgtcccgg acgacgcgcc
     6901 gaccgtggcg ttcgtcgtcc ggcgcgcccg ggagaccggc gtcgtccgcg tcttcccgat
     6961 cggcgccgtc tcgaagggcc tcaagggcgt ggagctctcg gaaatcggct cgatgaaggc
     7021 cgaggggatc gttgcgatct ccgacgacgg caagccgatc gcgaacggga acctcatgcg
     7081 ccgtgccctc gagtacgcct cgatgttcgg cctgcccgtc gtcgtacacg aggaggacgc
     7141 cgcactcgtg gggccgggcg tgatgaacga aggctgggcc tcgacgcgac tcgggctcgc
     7201 gggctggccg aacgtcggcg agagcgcgat ggtcgcccgc gacgtccagc tcgcggcgct
     7261 cacgggcgcc cgcctgcacg tcgcgcacgt ctcgacgcgt gagggcctcg aggcgatccg
     7321 cgcggcccgc aaggccggcg tcccggtgac gtgcgaggtc acgccgcatc acctcctcct
     7381 cacggacgag gacgtcgcgc gctccggcta cgacacgcgc ttcaagatga acccgccgct
     7441 gagatccgag gccgaccgga acgccctcgt cgcggcgctc gtcgagggga cgatcgactg
     7501 cgtcgccacg gaccacgcgc ctcaccacga ggacgagaag aacgtcgagt tcgccgacgc
     7561 cccgttcggc gtgaccggcc tcgagacggc gctgcccgcg ctcgtcgacg ggctcctcgt
     7621 tccgcgcgcg atcacgctcc tccgcctcgt cgaagttctg tcgacggccc cgtcccggac
     7681 gttcggcctc ccgtacggga cgctcgccga gggcgctccc gccgacgtcg tcctcttctc
     7741 gacgacgaag tcgacgccgg tgacgcccga cggctttcag agccgctcga agaattctcc
     7801 gtggctcgcg cgcaccctga agggccgggt ggaaaagacc tgggtgggcg gtcgggaggt
     7861 ctactcccgc gctcccgggc cggcacgatg agctcgcgcg aggactccgg ggcgtacctc
     7921 aagccggtct cttcggaggt cccggtcgct gcgggcaagc cgaagcggat catcatcgtc
     7981 gagcacgagg agctcttcgc cgaggtctgg cgcgccttgc tcgtgacgcg ctacggaccc
     8041 gccatcaccg tggagaccta ccaggacccg ctcaaggcgc tcgaggcgtg cggcccggac
     8101 gtgtcgctcc tcctggtcga cctcgagctg ccgctcttcg gcgggcggaa gatgttcgag
     8161 ctcgcgaagg agcgcggcgt cgcgccgcgg cggatggtca tcctctcgac gcgcgacgcg
     8221 aacgagctcc accagctctt cccggacggc ttctgcctcg ccgtgatcaa caagcacgag
     8281 ccgaaccagc agaacgcgtt cctgatgatc ctcgactcga tcgtcaagcg gcactagaac
     8341 tagcggtcga tctcgatctc cgggtcgttg gggatgtatt ggccgtcaac gagcagcgaa
     8401 atcgtgtact tgtatcgctt gtagggtttc gtcgcggtcg cctgatcctt ctcgcgggct
     8461 ttcggatgaa tttttggcga gcgaatgtgc ttgcactcgc tctcgctcca gcgctcccaa
     8521 gagccgtcga acggatcgtc cgattcgtcg gtcatgtgga cgttgaggag ccccgccccg
     8581 cagtatttcc agtgggcgcg atgcaactcc ttcttggaga gcggaagggg gtccttgtcg
     8641 ggcacgggtt ttcccgttgc atcccgcgtg atcgtaatgc cgacatccgt gtagttgttc
     8701 aatgccgaag tcgccgtcgc tgtcgggacc ttgggcgcgt ccggcgcgcg cccgcatcca
     8761 agtaccaggg caagcggcga aaagagcagg gctccggtgc gagagccttt cgcggtgaga
     8821 cgcatttcaa ggcctccttc tcttgtccgg gttctcgggg gcgggcatcg tgtgcagccc
     8881 gaagcccggg atctcctgcg gatgggttga tgcccgcaca ttatcccccg atcgtccgat
     8941 tcgtctccag ggtcctcgcg gccggcgtcg tcagggcttc ggcggcggag gctggacgag
     9001 gtcggggacg ttcgcgacag ccgccgggtc cggaggcttc acggcgaaaa cctccgacag
     9061 gaacgcgagg cggtcgcggc gcgcctcggc gttcagggcg tgcggcgcgt cgtagatctt
     9121 gagcgtcttc ggctcgctca cgttcgcggc gtactccttc gcccgcgccg gcgtcaggaa
     9181 gtcctcctgc gtcgcatact gcaggaggac cttcgccggc gccgccttcg cgacgtaggg
     9241 gccggggtcg gcccacgcgt ggagggccat gaacgcgtcg aagcggacgg gccccacctt
     9301 gtcccggtac ttccggtact cggcggtgtg caggtcgacc tggtccgaca gcgcgcccgc
     9361 catgaggacg aatgccttga aacggcggtc gacgcccgag aggatcgcgc cgaccgtcgc
     9421 gttgtagctg tggccgacgt acgcgaggcg gcgcgggtcg acctcgggcc gcgcgagaag
     9481 gaggtccgcc ccgcgacgca tgtcgacgac ctgctggacg aggtcgtcga actgcttctc
     9541 gttgagggga gtccggtcgg cgacgtggcc cgggcgggcg acgggcccgt ccgtcagaag
     9601 ggagacgacg ccggagcgcg cgagaacgac ggcctcgtcg agaaactgcc tccggctttt
     9661 catcggcgaa tttccccagt accagtgccc ccagaggacg gccgcgaacg ggccccttcc
     9721 ggccggaacg acgaggtacg cggggacccg tccgcccttc gggctcgcgt acgtcaggtc
     9781 ctggatcacg acgtcgcccc gcgtctcgac tcccgtcgtc acggcgtcga tcgaggcctt
     9841 cgggtcgtag gcgaagtgcg actcgggggc cggtgtgggg gttgcggccg cagcggcgaa
     9901 gaggacggcg gcgagggtgt tcgtcatggg tgtctccccg tgatgaggcg cgccgagcgg
     9961 ttccacgtca ggtagctcca ggcccactgg acgaggatca ggacgcgatt cccgaactgc
    10021 acgagataca tgaggtgaat gaagagccag gcgagccacg ccgcgtaccc cgcgaagcgg
    10081 acgaagccga ggtcggcgac cgcgcgcccc cgcccgaggg tggccatgct ccccctgtcg
    10141 cggtactcga aatcgcccgg ccctggccgg ccctcgagcc gcgcgaggat cgatcgcgcg
    10201 acgtggcggc cctgctgcat cgccaccggg gcaacgcccg gcaggggtct gccgtccgcg
    10261 ccgggcagcg tcatgaggtc gccgatcacg aaaatctccg ggtgaccggg cagcgtcaga
    10321 tcgggcgcga cgcgcacgcg gcccgcgcgg tcggtttcgc cgcctgcgga ccgggtgagc
    10381 gcggccccga ggggggaggc cgcgacgccc gcggcccaga gaacggtgga ggcctcgatc
    10441 gtctccggac ggccgtcgcg gacgacgacc accgcgccgt cccgcacgtc cgtcacggcc
    10501 gtcccgagcc gtacgtcgac accgagcgac gcgagggcag cctgcgcctt cgaagagagg
    10561 tcctccgcat aagaggacag gacgcggggc ccggcctcga cgagaaggat ccgggaatcg
    10621 gcggggtcga tcgtccggaa gtcgtcccgc agggtgcgcc gggcgatctc ggccacggcg
    10681 ccggcgagct cggctcccgt cgggcccgcc ccgacgatga cgaacgtgag gagggccctc
    10741 cggcgggccg catcggcggt gatctcggcc cgttcgaagg cgaggaggat gcggcgccgc
    10801 atttccgtgg cgtcctcgat cgtcttgagg cccggcgccc accgttccca ctcgtcgtgg
    10861 ccgaagtagg cgtgcgacgc gccggacgcg acgacgagga cgtcgtacgg gatctccccg
    10921 gcgtccgtga gcgccgcgcg ccgcgccagg tcgaacccgc gcacgtcttc gaggaggacg
    10981 cgcgtgttgc gctggtgccg gacgatcgac cgcagcggtg ccgcgatgtt cgcgggggag
    11041 agcgctcccg tcgcgacctg gtagagaagc ggctggaaga ggtggaagtt gcgccggtcg
    11101 acgagcgtca cgtctgccgc ggcgccgcgc agcgctcgcg ccgtgtagag gcccccgaag
    11161 ccgccgccga cgatcacgat gcgaggccgc gcggcgctca tgagaacatc attctaggtg
    11221 tgccgggact tcgcatgaag agcgctctcg acgtcgcgac tgcggccgcc cgcgttgccg
    11281 gggaggtgct cgtcgggcac ttcggccggc tcgaccccgc ctccgtcgag gagaaggccg
    11341 cgaacgacgt cgtgtcgatc gcggaccggg agagcgaggc cgtcatccgg agcgccgtcc
    11401 tcgccgcgtt tccctccgac cgcttcctcg gcgaggagac gggcctctcg ggggtcgacg
    11461 aaaccgcgcc gtcctggatc gtggaccccc tcgacggcac cgcgaacttc gtgcgcgggt
    11521 tcccgcactg ggccgtctcg atcgcgcgga cgcggggcgg cctcctcggc gagatcgagg
    11581 ccggcgtcgt ctgggacccc gtcaagaacg acctcttcac cgccgagctc ggatcgggcg
    11641 cgttccgcaa cgggcaccgg ctccgggtgc cgccgcggcc ggacctgcgc agtgcggcgc
    11701 tcgcgacggg cttcccgttc cggcaggcgc acaagatcga ccgctatctc gcgatcttcc
    11761 gcgagctctt cctcaaggtg cgatccatgc ggcgggcggg gagcgcggcg ctggacctcg
    11821 cgtacgtcgg cgccgggatc ttcgacgggt tcttcgagtt cggtctctcg ccgtgggact
    11881 cggcggcggg cgcgctcgtc gtgacggagg ccggcggccg catttcggac ttcgacggcg
    11941 gggacacctg gcggacgcgc gggaacatcc tcgccggctc gcccggcgtc cacgcggcgc
    12001 tcgtggcggc aatgcaggag atgggagttc aagagaaaga tctatgagcg cgcgggggat
    12061 tctgctcgcg ttctgggcgg cttcggccgc gatgcgagcg gagtgccaga ctcccgactg
    12121 gttcgacgcc ttccggggca aggcttactt gcagggggac gcgagctacg tcgcgcgcga
    12181 gaagtcggca gcgcccgact cgccgccgct cttccgcgcg cgctgggtgg tcgtgcgata
    12241 cgaagggttc tccgagaagg agcgcgaagt gtggccgtac cggaacctcc tcctcgtgac
    12301 gatgccgggc ggcgcgaagt acgtggtcga gtcggccttc ggcttcgtgg acgagaggca
    12361 cctggaggag gaccggccct ttcatcgggt tgcgtcggcc cgtgccgtgt tcgagatgtg
    12421 gtcggcgggc ggcttcgagg atcccgcggc cgcggcggac gacccgtgcg ggggatcgcg
    12481 gcagaaggtg cgtgcgccgg gaggaagcct cgatttcatc ctgacggatc tttcgagccg
    12541 tacggtgagg acgagccttt cggagatcac ggcggggacg ttcgacgcga acgagcgacg
    12601 cgacctcgtg accctgctca aggtcgcgcc ggacggcgct ccctctctgc cgagggacac
    12661 tttcggcctg aacgcgcgca atgcgctctt ctccgtgacg gtcgcattcc gaggcgacgt
    12721 cccgccggtc gctcaacgca ccgtcgtcct cgcgccggat ccggcgcccg ccggcgacct
    12781 cgcctcgtgg cgcgccctcg cccacctgcc gctggacctt ccaccgttcc ccgcgatcgt
    12841 gccggagaac ccgatccgat gaagaccggc ggccctctct tcctttccaa gaaagtcttc
    12901 tttcttctct ttctcttctc ctcggtcgcg cgagcggcgg aaccgccgaa ggtcacgctc
    12961 gagcgcatcg tcgacggccg cggcaaccgc gtctgcgacg tcgcaaaggc ggagctgaag
    13021 gccgagggca cgcggcgctc ggcctgggtt ctgcgcggcc tcttccggag cgacctcgtc
    13081 ctgaccctcg agacggccgg ccgcgcggag atccacgaga tcggtttcgc gggccgcccc
    13141 gcgctgcggt tcacgagccc ggcgccgggc ggacgcgtga cggccgcgtc gcccgcgtcg
    13201 tccttccgct acttcgaggt cgacgtcgcg acgaagacgg tgcgctgcaa cctcggccgc
    13261 ctcgcggagg agacggacta cgagctcctg cgcgccgccg ccgaatactt cgagcaggag
    13321 ctcgaggagg acgggctcgc ctccgaggaa gccccgctcc tcacgctccg cgccgtcgtg
    13381 aaggctcctc cccgtgcgtc gccgccgatc cgcaagcgcg ttccctggac cgagggcggc
    13441 ccggaggccg acgagctcgc cgcggccgcg cgcgcggccg tctcgcgctg acctaccagg
    13501 gcagtccgcg gacctcgcac gcccggcggt agaccgcctc gtagcgctcc gcggagcggt
    13561 cccacgagaa gtcctgcgcc atcccgttct cgacgatccg ccgccagcgg tcgggctgga
    13621 agaagacgtg ctgggcgcgc aggatctcgg ccgcgagcgc gtgcggcgcc ggtgcgtcga
    13681 acgcgaaccc cgtcgcccgg tcgaggttga agccgtcgta cgggacgacc gagtccgcga
    13741 ggccgcccgt gcgccggacg atcggcggag taccgtacgc gagcgcgtac atctgggaga
    13801 ggccgcacgg ctcgaagcgc gacggcatca cgaacgcgtc ggcgccggct tcgatccggt
    13861 gcgagagcgc ctcgtcgtat ccgacgaacg ccttgaagtg ccccggatgg cgcgcctcga
    13921 ggagcctcat cgagccctcg tacgccggct cgccggatcc gagcatcacg atcctcacgc
    13981 cccgccggat cagctcggga gcggactcga ggaggaggtc gaagcccttc tggtccgtga
    14041 ggcggccgat cgcgccgacg aggaagccgg agtcggacgg gtcgaagccc gccaggcggc
    14101 agaggtcggc gcggcacgcg gccttccccg agaggtcgta cgcggcgtac cgggccgcga
    14161 ggtgcgcgtc cgtggcggga ttccaggtcc cccggtcgat cccgttcagg atcccgacga
    14221 cccggtcgcc tcgcgccctg aggatcgcgt cgaggccgaa gccgccctcc ggcgtgcgga
    14281 tctcgtcggc gtacgtgggc gagacggccg tgatcatgtc ggccgcgacg atcgcgccct
    14341 tcaggaggtt gacgtttccg ccgaactcga cgccgccgct cgaccaggcc tcggccggca
    14401 ggccgagggc cgggaactgg tcgcggccgt agacgccctg gtagccgagg ttgtggatcg
    14461 tgaggacggt cgcgacgtcg tcgaaccagc ggtcccgcca gttgctccac gccgcgagca
    14521 tcgccccgag cgcgggatgc cagtcgtgga cgtgcaggat ccggggcttg cgtccgagcc
    14581 gcttcagcgc ctcgatcgac gcgcggacga agagcgcgaa gcgccagagg ccgtcgtcct
    14641 gcccgcgctc gttcggggcg tagatctgac ggcggtggaa cagcgggtcg tgcgcgacga
    14701 agtagatcgg cacggtcgtc ccgggaagga ggtcccagag gaggccgacg cgatacggca
    14761 ggccgaggat cgagacgtcg aaggacggca tcgcctcctc gggccacgcg cggcgcttgc
    14821 gataccactc ggccgcctcg aggtggaacg gaagaaacac gtcgacgtcg tttccgccgg
    14881 acgcgagggc cttcgggagg gcgcccacca cgtcgccgag cccgcccgtc ttcgcgaacg
    14941 ggacgcactc ggcggcgagg tgcgtgattc tcaggcgacg cctcagttca cgatcgcggt
    15001 cgccgtcagg gttcgggaag acgtcgcgac ggtgatgacc gcggtgcccc gcgcgagccc
    15061 gacgacggcg ccgttcgaga ccgccgcgac cttgggctcc gagctcgtcc acgcgagcgc
    15121 cgcgtcctcg atcgccatcc cgctcacgtc gcggacggtc gcgttgatgc gctgggtctc
    15181 gccgatcttg aggatcagcg tgaggggact gatctcgaac gttccgatgt cacggtgcag
    15241 cacccggacg tccgacgcgg agctctggtc gttcccgagg ctcgcgatga tcgtcgtccg
    15301 cccctcgccg gccgacgtca cgattccgtt cgcatcgacc gtcgcgatct tcggatcgcc
    15361 cgacatccac ttcggcttga ggttcacggc gttgcccttg gcgtccttga tctccaccag
    15421 gagcgccatt ttcgtcccgg gcgggccgac gagcgtcatg cggttcgggg acacggccat
    15481 cgaggcgacg tccacgactt ccacggaggc gttgcccgtg acggcctgcg ccgccgcggt
    15541 gagggtcgtg cggccgggag cgagggagcg cacgaggccg ttcgcgtcga cgctggcgat
    15601 cttgggtttg tcgctcgtcc aggtgacggg aatgccgggg agaggccggc ccttcttgtc
    15661 gacgacctcg aacttgacgg tctgcgagcg gccgggcccg tagaaggtga gcttcgccgg
    15721 agtcacgcgg atctccgcgg gcttcggttt gcagccgagg gcgagcgccg cgccgagcgc
    15781 gactgcggcg ccgcgcagcg ccacgacatg tcttgcgttc attggggcga ttctagcgga
    15841 tggcgccacg ccggccgggg gatggaatca tggcggccat cgccgcggcc tgctgggcgt
    15901 tcaggcggga ggcgggtacc tgaaagagcg agcgcgccgc tgcctcgcag ccccacgtgc
    15961 gctcccccca ctcgatggcc gaaaggtaga cctcgaggat ccgcttcttc gggacgcagg
    16021 cgtcgaggac gagcgcgatc gaggcctcgc gggccttgcg ccaccaggtc cgctctccgg
    16081 acagccagag gttcttcgcg agctgctgcg tgatcgtcga ggcgccgtgg acgcgcgggc
    16141 ggcggtcctt cgcgtgccgg cgattgtacg cgcgcgcctc cgcgaccgcg atccagtcga
    16201 tcccatggtg ctcgaagaaa cgcgcgtcct cggcggcgac gaccgcggcg acgagggcgg
    16261 gggagatctg cgagagcggg acgggccggc ggtcgatgga gtcgctcttt ccctcgcggc
    16321 gcagccgttc ctggcgcgcg cggatgaacg cggtcgtcga cggatcacgc cagcggagga
    16381 ggattatttg cggaagaggg atgaagatga agaaggagaa gaaaaagaga ctgcttagaa
    16441 ggtaaagagg gagacggcgc acgaagcggc gccatgcagg gccggcggcg tctcttgttc
    16501 cttcagaaga aatcttctct tctcttcttc gttctcttct tcaaccaaga atctcggtca
    16561 gcgccgcgcg caggcgctgg acgccgagtg cggcctcgtc cttcgtgagc gtgagcggag
    16621 ggcgcaggcg gatcgcgcgc tcgccggatg tgagcgccat gacgtggtgc ttcttcagcc
    16681 agcacgagag gacgtcgccg cgcgtcttgc cgtccggcag gtcgaacgcg atgaagagcc
    16741 cccgtccgcg gatgttcgtc atcttcccgg ggaactcggc gccgagctcg acgagcttgc
    16801 cgaggaggaa ctcgcccgtc gtcttcgcgt tctcgacgag gttctcctcg accatgatct
    16861 cgaggtactt gcggcagcgc accatgtcga cgaggtttcc gccccacgtc gaattgattc
    16921 gggaggagac gttgaagacg ttctcgggct cttccatgat ccggtcgttc gaggcgaagc
    16981 cgcagacctg cgtcttcttg ccgaacgtga acatgtccgg ctcgacgccg agcccctgga
    17041 aggcccacat ggaccccgtg aggcccatgc ccgtctgcac ctcgtcgagg atgaacagca
    17101 agtcgttctc gtcgcagatc gtccggaggc gcttgagaaa ctcgggacgg aagtgattgt
    17161 cgccgccctc gccctggatc ggctccatga tgagcgccgc gacgtcgtcc gggttctccg
    17221 cgagagcctg gtggatctgg cggatcgcga gctcctcgac ggcggccgtc cgggcgagcg
    17281 actcgggcgt gagcgggaac gcgagcttcg ggttcacgac gcgcggccag tcgaacttcg
    17341 ggaagtacat cgtcttccgc gggtcggacg tgttcgtgag ggagagggtg tagcccgtcc
    17401 gcccgtggaa cgcctgctcg aagtggagga ccttcgcgcc gacctcccgg cgatggccct
    17461 tgcggaagtt gcggcgaacc ttccagtcga atgcggcctt gagcgcgttc tcgttcgcaa
    17521 gggcaccgcc ctcgacgaag aacatgtgct tcgcgtgcga ttccggcacg gcgaggcgcg
    17581 cgaaggtctc gacgaagagg gccatctgct cggtgtagat gtcgctgttc gccggcttcg
    17641 tgacggcggc ccaggcgagc tcgtcgagga actcgggagt cgtcaccttc gggtgcgcgt
    17701 agccgatcgg gcacgtcgcg aagtgagagt agaagtccag gacctcgact tcgtggagcg
    17761 agtcgtagat gaagttgccg tgcgagcgct tgaggtccat cacgacgtga tacccatcca
    17821 cgagcatgtg gctcgagagg gagtcgagga cctgtttcgg tccgaggttc gagatcaggc
    17881 ggggggagac gtgagaagcc atgcgagcat gataacccgc ctcgatgcac tcgcgaaccg
    17941 tatgacagga ctcgggttat cgctccggga tcaggtcttg aggatgaccg ccagcgagtc
    18001 ctctccgggc tcgtcgtcgc ccgctccgac gacgcagttc ttgccccggg tcttggccat
    18061 gtagagcgcc gcgtccgcga gcttgaggag ctcgttcttc tgctcgggag cggaaagcgc
    18121 ggggttcacg ttctcccgcc aggtcgcgac gccgatcgac atcgagatca cgttcttcag
    18181 gttcagcgcg ggctcgccgg gcccgatcga catcggcagg aagacccagt cgttgatcgc
    18241 cttccgaacc gactcggcga tctccatcgc cctctttcgc gtcgcctcga ggaggatgac
    18301 ggtgaactcg tcgccgccgt agcggcacgc gagcgacacg gtgtccgcga gcgttctctg
    18361 aaggatgaac gaaacctcgc gcaggacctg gctgccggcg aggtggccgt gcaggtcgtt
    18421 gacgcgcttc aggtggtcgc agtcgaggaa gaggagggcg aggttcccct ccttcgcctc
    18481 cgcgagccgg tcgatctcgc gctggagctt ccggtggaag tagcggtcgt tgtagagcga
    18541 cgtgagggag tcgcggcgcg cgacctcgcg cgcgcggcgc tcgtcgagca ggttctggag
    18601 tgcgagcgag agcgtgtcgc cgaagagctc catgaggcgc tcgtcgcggg gcgtgaacgc
    18661 gggctcgctc tgccggttca ggagaatgag cgagccgcag acgtggttct cgatcacgac
    18721 gggcacggcg aggacgttca ggacgtcccg cgcgaggagt ccgtccgtct cgcgggagaa
    18781 cgcgtacgcg cccccgacct cgttcacgca ggtgcgctcg ccgcgggcgt agacgaagcc
    18841 ggcgatcccg tccaccggcc gcaccttccg gcccacgacg ccgtcctttc cctctcccgc
    18901 gaggacgatg atcgtgagct cgttgcgcga gcggtcgtcc tccttcgaga cggggtcgtc
    18961 cagcaggagc gcacacgtct cggacggcac gaactccatc gcccggttca cgagggtgac
    19021 gaggaagcgc tcgggtgccg tcccgctcgt ctcgtcgagg aggcggtgac gctccatgta
    19081 gcgcgtcagc agcttcgggg agatgacccg cgcgttccgg ctcaaggttt ccggcgattt
    19141 tacgcccatc gggcgccgcg ccgagtcggg ccggtcaggc gacgagcggc aggacgcgcg
    19201 cggcgcgctc gcggacctcg cggattcgag cgtcgtactc gccgagttgt ggcgcgaacc
    19261 ggaggaaatc gacgtcgtcg aggagcgcct tcacgtcggc cagccgggct tccgggaggc
    19321 cgcggtccct gagcgccgcg aggagttcgg aggctccgag cgtctcggag acgccgtggc
    19381 ggcgcgcgag cacttcgcgc agcgcgcggt ccacgcgggc ggcggcgcgc tccttggtct
    19441 cgcccggctc gggagcgagg gcctgccg
//
LOCUS       DAOMFY010000046        19254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_899_length_19254_cov_7.782541, whole genome shotgun sequence.
ACCESSION   DAOMFY010000046 DAOMFY010000000
VERSION     DAOMFY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19254
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_899_length_19254_cov_7.782541"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLB01_05955"
     CDS             <1..245
                     /locus_tag="PLB01_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HQQ76879.1"
                     /translation="PKSVDLQAGSGQFEAERGEVGRGGIVRDEAVRARAVARVEASAI
                     ALGLEPRGAVPSPITGADGNVEYLAAFRRNPATLSG"
     gene            242..1141
                     /locus_tag="PLB01_05960"
     CDS             242..1141
                     /locus_tag="PLB01_05960"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQQ76880.1"
                     /translation="MTVRKRSASRVRRVGLVTRYTSREALELSRKLDRRLTKRGYEVV
                     HDLESAAAREDGGGVTRTQIARSVDLLVTLGGDGTLLSVARHPAPNVPILGIDIGTLG
                     FLTACSPGDCDEILEEALAGTARLENRRLLAVSVAEGRRPARTYRVVNDAVLGKSALA
                     RIAAIRVDVDGRTVSRYRGDGLIVSTPTGSTAYNLSAGGPILEPTMPALVLSPICPHT
                     LSLRPLVLPDSVRLDLRVEEDATGVFLTLDGQEGFPVSAATRISISRARETVSLVRAS
                     ADSFWDVLAGKLSFGGERDNASR"
     gene            1151..5164
                     /locus_tag="PLB01_05965"
     CDS             1151..5164
                     /locus_tag="PLB01_05965"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQQ76881.1"
                     /translation="MARRPIPTLPQLSLTQVRLVRLFALLLLFSVLVLYGVFRSHRFQ
                     DALRRKTERVLSAQIGRKVTIGGFDLALLPPSFLVRDVTIANDRRSLAGPLFSAAEIE
                     IRGIPAVTSNRIELSKLRVVAPRLVIEVFPDGSTNVDPILRALRGGGGGGKDIQLHEA
                     VIQRASFRFREWKAEIDALLKDAAVTARAGRTLSVTHLVLACRQGSFRLENNEALEFA
                     VGAEITLAPGRAHLTGLRLKGPKISVEASGGVDDLRKPVFALVAKIETTGETLGSAFG
                     LGLPLGGPVRLNGTLRVGDPGGFRIRASFDLPASRLAVFPMSGEGTLRLDPKGLLVDV
                     TRASYAGGSLEALVQLERLDRPPLPVKIALRGKGIDFEQFFADIGLPGTGMMARAGLE
                     ATLTFGPGGVEHADGAGRLTLTADPGRASAVKGRVPIPVAGGGPLRIRDGRILFDKLP
                     LATAGGAKVRLDGSLRFGSWEPDLTFDVTAGDLQEVERIAANWYPAIQNEPLSPPLNL
                     GGAGHVSARLTRAFSDPHVEGRMEATDFVLRGVRFGETTANFTVDHNVATLAPFAARD
                     DGGTLALEGKIGFGGALHGQYRLDGLVGDFREWPLEKVLAFLDFDLPLSGRATGRLPL
                     DGVTPAIHGRIPLVLTDASIWGQKLDRLEGVLAFQDDRIAVEGATGRLGGATASFGGS
                     YRYADGAYKFDLDVKDVPAGRLAALQDAPVGGLLTGHASGEGLIDKPTLSADLRVADS
                     TYDGQPLARAGHPVEIHARADRGSWSLRAEAAEEGVLEILSPRDAGSAGRWRARAEFP
                     SLAPLAGLLGLPADAKFDGRLSAEASLTPGASAGELDGEGVISAAQFTAWGRTLTLRG
                     TTPLKVSGGRVSFERLALAEAVRPEAPSGVPTLVTLSGSAQFVPPRALDVSAIGSVDA
                     SLLGPAFAPLKVAGRITLDARLGGTAASPVVTGRAALDGVDVVSPDGGVAFESATGSL
                     LFSEGKVTISDLSLRFAGGTVDLGGSVNLDGWKPSGMRVSALVSHVKVSPFDGFRGTF
                     SGNLLMLGDTLPRAVRGELTFDRALYDRDFSIDLAALLQRKRVATVGANPTFLDPMTL
                     DVRLVAPPESIEVRTNVARLKASGELFARGTWGHPLLFGEIRAEEGGRLTLQGQRYDL
                     LSGRILFSNPIRIEPFFEMEARGTVSKYQVTFGLTGTAARLATRFSSDPQLSEAQIIS
                     LMATGDVPSTSVAGGPIGPAPTSSDESVSKAARELLASLASDAVTSRTKEFFRLDRFQ
                     IDPAFSTGSTFDAPRITVGKSLGKDFNATVSFVLSSNQQQIITLDYQLSRTAFLQARL
                     DEYGVYSLELRFRQRLR"
     gene            5161..7980
                     /locus_tag="PLB01_05970"
     CDS             5161..7980
                     /locus_tag="PLB01_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQQ76882.1"
                     /translation="MRAARGAAAALVLALGAARAGAEPPPEGLLWGRLVTTLSFRGDG
                     PVDEKRMAGLTEITPGKRLTEGAVRISLRNLFATRRFSDLSVEGAPFGEGVAVVVAYS
                     AAPRIASLAVTKGIPARGRALDAIGLHPGDPWESDRGPGYETAVRRVLKEEGYFESAV
                     SVSVDAGPDETSVNVSFVVRPGPKAVAAAPEWNGSLGTLPPSALTAKAKLKAGRPYKR
                     KTALEDAERFENELHRRAYSRAEVRLGDERYDPATASVSPRYTVFVGPAVVLEVTGED
                     EKLVRKHADSPWRKGEPPDEDAVERLRAALKRSYEESGYAKASVKVAFDTQPDKEIVR
                     FEIVKGARWSVERVDVTGAVSLPPRAVQSAFETRPRGILEAGRYVSDEAARDRDALAT
                     LYREGGWRDVRVAAPAVVEGRGEHTLDVTFAVDEGVRTVVDRMRVEGMRLLPEKDLVP
                     RLSIKPGVPYSESAVSADAALLQSLYVDKGFVDAKVEATARFSGPEPPQGKRADVTYT
                     VTEGSPVLFGKTIVRGNSRTKPFIIEDRLANAEGDPFSLTKLLDTQQALARLGVFERI
                     DITTFETDPETMSRSVLVTVAEARPWGLTYAVGAEYNPQNNASLSEQLSLRLSLAVTY
                     NNLFGRALEVGVEGRASNNDPRIIFTARDRSLFGGKVPLSFAIYNTKDVPSPAYDVKR
                     KGTFLQGEYRLSKSLRTGLRVQYELVEPSSDPGLGADQRGNTESRIASVSSGVTWDRR
                     DDPLNPRNGFLLGTDLKYAFPLFAADAHFFKLLSQAGLYRGWGSTRVAFSLRGGVIWN
                     YAACPDPTGETCAPNLIIPVPERFFAGGTSTHRAFTRDNLGITPQTLNSDGVGVGGNV
                     LLIGNAEWRIPVASGFEVALFVDVGNTWADPKNVSFGEVRTGVGVGLHYLTPVGPLRL
                     EYGIKLDRKPNEDAGAFAFAVGYPF"
     gene            8004..9350
                     /locus_tag="PLB01_05975"
     CDS             8004..9350
                     /locus_tag="PLB01_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76883.1"
                     /translation="MPRFTVLETSALFSNRVKYDYLFELEMWLKGLERFFQIDCLPLS
                     AFERSHASLKNYAEEVAAARSGVAHMGLIATQLMGEGQEDLASFLLYLDTQVQRPGGR
                     RRGDGTTELARVVETLDDFTKILDELAKAPFIPLQTYLSLGRVVVGTLRQDPNLGVLF
                     RENLRPVLDRESKQSLAKVLGSLPDPSHRGALATLFVALFKGLRVAERVSAAAEGPAT
                     RKRALLLFSFLKSESDSFVAYVRRRLRPRFEDGTKEAEGLDRLASAFEAETRKAMDVE
                     LVGVALMKDPDAVLARLGDGAGLFRDLFQQSVIGLAETFSSGIDARSLFPEYRARLDQ
                     SLRLRDELGHLSRATQDFLKTPDKKTLRALVTEIESFRRGGMRYLMFKDWSLFERFHA
                     GFSRERVPRAFLPAANQFEGFLRTLLKEVGKRAVLADHPFRSPRPDAGSGGASRYN"
     gene            9399..10601
                     /locus_tag="PLB01_05980"
     CDS             9399..10601
                     /locus_tag="PLB01_05980"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HQQ76884.1"
                     /translation="MGFVSVVDAVAAYKKGQFVIIVDDEDRENEGDLCLAAEHVTPAA
                     INFMAKEGRGLVCVSLTEERCDELDLHPMVEQNTSNYGTAFTVSVEARGKTTTGISAA
                     DRAATVLTLVDPKSRPHDLLRPGHTFPLRAKKGGVLKRAGQTEASVDLARIAGLAPAA
                     VICEIMNEDGTMARVPDLLLVSEKHGIPIVSVADIIRYRMRTERLVHRIAAPVLPTPF
                     GEFRAVAFRSDLTNEEHVALVMGTWTEDEPVLVRVHSACLTGDVFGSGRCDCGAQLQR
                     AMAQIANEGKGVLLYLLQEGRGIGLFNKLRAYELQEQGLDTVAANEKLGFAPDVRDYG
                     IGSQILRDLGVKKMRLMTNNPSKYVAIDGYGLEIVERVPLEIPPTDGTRDYLATKKAK
                     MGHLLKLV"
     gene            complement(10667..11368)
                     /locus_tag="PLB01_05985"
     CDS             complement(10667..11368)
                     /locus_tag="PLB01_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76885.1"
                     /translation="MAASSITVIVPVGAGEAPPPGDFFERVRGAGPPVSLLVAADARI
                     RPETRGAFQHAGAALFAFEEIPRGARLARAARSSSSAEILLFLHCDTLLPVGWETEVR
                     AALTRGAAGGAFRLGFSGGDPRLAWVAAWANVRTRITKVPYGDQAPFVRREVYERLGG
                     HRPWPFLEDRDLSIRLKQAEGRARIALLRARVETSPRRYLEKGVWKTVRGNWEILRRV
                     RGGESPAELAKRYRD"
     gene            11386..12000
                     /locus_tag="PLB01_05990"
     CDS             11386..12000
                     /locus_tag="PLB01_05990"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HQQ76886.1"
                     /translation="MAYGFVLVGGRSVRMGRDKTLLPYHGQPMAYHQAAKLARVCGRV
                     ALVGKHPELFTGSPYPFIEDGAAPTAAIFGVAAALAASPDETNLILAADIPRIGEGFL
                     AALLEVADAIPADAVVPVSGGVAQPLCAVWRRSAHPPLLARLAAGDYALVGALQQIRT
                     VLIPEAATAALPGGHPDNFLNVNTPEDYEHAEKDAGAAAQPPRL"
     gene            11954..12439
                     /gene="moaC"
                     /locus_tag="PLB01_05995"
                     /pseudo
     CDS             11954..12439
                     /gene="moaC"
                     /locus_tag="PLB01_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
     gene            complement(12447..14156)
                     /locus_tag="PLB01_06000"
     CDS             complement(12447..14156)
                     /locus_tag="PLB01_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQQ76887.1"
                     /translation="MKRTGAELVVKALEDEGIALAFGIPGTHNIELWDALASSESVRS
                     VLVSDEQSASFMADAACRASGVMACVNVVPGAGLTHAMSGIAEAFLDGVPMLVLGCGI
                     RRDTGKAYQLHDVDQLALARPVTKAQILPPSGADLYDAVRRACRIARSGTPGPVFVEV
                     PADQYLFRHETDVVPPAPEPRAALPERSIERAAEILRAAKRPLLYVGLGAARADLVAL
                     AERLEAPVSTTFQGKGVFPESHPLSLWPGFGDAAPAFARDVAASCDATLAIGCRFGEV
                     ATGSYGMTPPRPLVHVDIDPAVPGRNVPTDLAVAADAAAFVAALLPRLDRREPDAGLR
                     DRIRSGRAEVDAELARPSEGRVSPHLLLRALQATFGPETVFTSDSGNGTFLAVEGLRL
                     ERPGRLLAPVDFSCMGYAVPAAIGAKLARPASPVVALAGDGAFLMTGLELLTAAQNGL
                     GVAVFVLRDRELAQIAQFQATALGRKASSTLADHDLLALARALGAEAVAISTDGEIRS
                     VVSNAKEIADAGRPVLVEVAIDYSVKTFFTRGVVKTNFLRLPWADRLRFALRALARKL
                     PGR"
     gene            complement(14153..15676)
                     /locus_tag="PLB01_06005"
     CDS             complement(14153..15676)
                     /locus_tag="PLB01_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQQ76888.1"
                     /translation="MNARHYDVVVIGSGAGGGTVAEGLAPLARAGYRILVVEQGARLA
                     ESEFTGKEDDMADALYEDGGGFLTADGAMTLAFGRVWGGSTVVYTGTSLVAPRRVIES
                     WRVPGLAHADVESRSRKFMAENGVHLLEPELLNDNNRLFVEGCSKAGLKAEQFPLNLR
                     GCRGSSLCNLGCPNGAKQGTHVVQLPAALAAGVQAVTRAEALRVEEGRVVIRVSAPAA
                     GEKGAPSEWSPGEYAVSAKAIVAAGGSIGTTALLLRSGLGPSLPRLGAGFTCHPAHIL
                     VAEHERPITNDVGHPKSFYLDRAVEEGYVLETCMYFPFITAKNLTGFGVPHATLMRAF
                     PRLQMILVLACDKAVPENRISVDTDGKAVVHYSFTKETVASLVRATRAAARIFFAAGA
                     LRVHAPSADPPLLERSEAGAVDSRVDERHFRTGRISVSAAHLMGGCGMGSSPADSVTD
                     SWGRVHGRPGLFVADSSLFPDSLEINPYLTVMALADRAAERIREDAGALLGAPGRAR"
     gene            complement(15673..16071)
                     /locus_tag="PLB01_06010"
     CDS             complement(15673..16071)
                     /locus_tag="PLB01_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76889.1"
                     /translation="MLLAPVRNTFRAVAVTVVPMASALDEPGWNALEALVEKTLAKKP
                     AGMQRQLVTFVRAIEHLPRLRWGRPFTALSPDERTRFLSGFEHSPVLLLRRGFWGLRT
                     LVFLGYYARPEAAAAIGYRADARGWEARRA"
     gene            16144..17157
                     /gene="moaA"
                     /locus_tag="PLB01_06015"
     CDS             16144..17157
                     /gene="moaA"
                     /locus_tag="PLB01_06015"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQQ76890.1"
                     /translation="MGRTLVDGHGRRVTYVRISITDRCNLRCVYCMPEDQKFRPLPHL
                     LTREEIRRSARLLRDFGVEKIRITGGEPTTRPDLVDIVRDLGEIGFPGGIALSTNGVL
                     FPRMAHDLLAAGLTKVNVSLDAAEAEHFRKMARRDHYAPVRESIELALALPFHSVKVN
                     AVVIRGFNDDQILPLAALTKDKKLGVRYIEFMPFGDNTWNESDWIGWREVWDRLAAAG
                     FVLTPIEKTLVAGPADEYSVTSPAGSHTGSIGFITPVSDRFCDTCNRMRLTQDGFLKG
                     CLFGHADVDILKPMRAGANDEEIAALIQRAATVKEERHPMFVGMDKAMLPLHARNMSE
                     IGG"
     gene            17165..17674
                     /locus_tag="PLB01_06020"
     CDS             17165..17674
                     /locus_tag="PLB01_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HQQ76891.1"
                     /translation="MRVAVLTISDRSYHGERADKTGPAVAEAACRLLPGASVVEQTIL
                     PDERDDIEWNLKRIADTASADLVLTAGGTGLSPRDVTPQATQAIAGYDVPGIGELMRA
                     ATRAALPVAILSRQVAAVRERTLIVNLPGSPRGAVECLEAVADVLPHAVATLRNEAGD
                     EHPARLERA"
     gene            complement(18517..18798)
                     /locus_tag="PLB01_06025"
     CDS             complement(18517..18798)
                     /locus_tag="PLB01_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76892.1"
                     /translation="MNPWLQLLPSGQWDQLRQLHQSTRWRRLLQSAQSDQRHQSLRSR
                     RSHPWLLSDQRHLSGRWLLLRRSVQWRPWDQQRLWDRPVQRVRWRLLST"
     gene            complement(18985..>19254)
                     /locus_tag="PLB01_06030"
     CDS             complement(18985..>19254)
                     /locus_tag="PLB01_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76893.1"
                     /translation="HPSAQSGQRHQLRRSNPWRQWRPLLRSHQSLPWDQWDQQRQLRR
                     SNPWLRWRPLLQSARSDRLRPSNQSDQRHQSLQSGPWLPSRRQLP"
BASE COUNT         2813 a         6794 c         6834 g         2813 t
ORIGIN      
        1 gcccgaagtc cgtggacctc caggccggct ccgggcagtt cgaggcggaa cgcggggagg
       61 tcggccgggg cgggatcgtc cgcgacgagg ctgtccgggc ccgcgcggtc gcccgcgtcg
      121 aggcgtcggc gatcgcgctg ggcctcgagc cccgcggcgc cgtgccgtcg ccgatcacgg
      181 gcgccgacgg caacgtggaa tacctcgccg cgttccggag gaatcccgct acactttcgg
      241 ggtgacggtc cgaaaaagga gcgcctcgcg cgtccgccgg gtgggcctcg tcacccggta
      301 tacgagccgg gaggcgctcg agctctcccg caagctggat cgccgcctca cgaagcgcgg
      361 ctacgaggtc gtgcacgacc tggaatccgc cgcggcccgg gaggacggcg gcggcgtgac
      421 ccggacgcag atcgcgcgct ccgtcgacct tctcgtgacg ctcggtggag acggcaccct
      481 tctctcggtc gcccgccatc ctgcgccgaa cgtccccatc ctcgggatcg acatcgggac
      541 cctcgggttc ctgacggcct gcagccccgg cgactgcgac gagatcctcg aggaggcgct
      601 cgcgggcacc gcacggctcg agaaccggcg tctcctcgcg gtgtccgtgg cggaagggcg
      661 ccgcccggcg cggacgtacc gcgtcgtgaa cgacgccgtc ctcggcaagt cggcgcttgc
      721 gcgcatcgcg gcgatccgcg tcgacgtcga cggccggacg gtgtcgcgct accggggcga
      781 cgggctcatc gtctcgacgc cgacgggctc gacggcctac aacctctcgg ccggcggccc
      841 gattctcgag ccgacgatgc cggccctcgt cctgtcgccc atctgccccc acacgctgtc
      901 gcttcgcccg ctcgtcctcc cggactccgt ccgactcgac ctgcgcgtcg aggaggacgc
      961 gacgggtgtc ttcctgacgc tcgacggaca ggaggggttc cccgtgagcg ccgcgacgcg
     1021 catctcgatc tcccgcgcgc gggaaaccgt ctcgctcgtc cgggcctcgg cggactcctt
     1081 ctgggacgtc ctcgcgggca agctctcgtt cggcggcgag cgggacaacg cgtcgcgcta
     1141 gcgaggggag atggcccggc gaccgatccc gaccctcccg caactctccc tcacgcaggt
     1201 tcgtctcgtc cggctcttcg cgctgcttct tcttttctcg gtcctcgtcc tgtacggcgt
     1261 cttccggtcg caccgcttcc aggacgccct gcggcggaag acggagcgcg tcctgtcggc
     1321 ccagatcggg cggaaggtca cgatcggcgg cttcgacctc gcgctcctgc cgccctcctt
     1381 cctcgtgcgg gacgtcacga tcgcgaacga ccgccggtcg ctcgccggcc cgctcttctc
     1441 ggcggccgag atcgagatcc gggggattcc ggccgtcacg agcaaccgga tcgagctctc
     1501 gaagctgcgc gtcgtcgcgc cgcgtctcgt catcgaggtg tttccggacg gctcgacgaa
     1561 cgtggacccg atcctccgcg cgctgcgggg gggcggaggc gggggcaagg acatccagct
     1621 ccacgaagcc gtcatccagc gcgcgtcgtt ccggttccgc gagtggaagg ccgagatcga
     1681 cgccctgctg aaggacgccg cggtgaccgc gcgcgcgggc cggacgcttt cggtcacgca
     1741 tctcgtcctg gcctgccgcc aggggagctt ccgcctcgag aacaacgagg cgctcgagtt
     1801 cgccgtggga gccgagatca cgctcgcgcc tggccgcgcc cacctcaccg ggcttcgcct
     1861 caagggaccg aagatctccg tcgaggcgtc gggcggcgtc gacgacctcc ggaagcccgt
     1921 cttcgcgctc gtcgcgaaga tcgagacgac gggcgagacc ctcgggagcg cgttcggtct
     1981 gggcctgccg ctcggggggc cggtccggct gaacggcacg cttcgcgtcg gcgaccccgg
     2041 cggtttccgg atacgcgcct ccttcgacct tccggcctcg cgcctcgccg tcttcccgat
     2101 gtcgggcgag gggacgctcc ggctcgaccc caaagggctt ctcgtggacg tgacgcgggc
     2161 gagctacgcg ggggggtccc tcgaggccct cgtccagctc gagcggctcg accggccgcc
     2221 gctgccggtg aagatcgccc tgcgcgggaa gggcatcgac ttcgagcagt tcttcgccga
     2281 catcggcctt ccagggacgg gcatgatggc gcgcgcgggt ctcgaggcga cgctcacgtt
     2341 cgggccgggc ggggtcgagc acgcggacgg tgcgggccgc ctcacgctca ccgcggaccc
     2401 gggccgcgcg tcggcggtga aggggcgcgt cccgattccg gtcgccggcg gcggccccct
     2461 gcgcatccgg gacggccgca tcctcttcga caagcttccg ctcgcgaccg cgggcggcgc
     2521 gaaggtccgg ctcgacggat cgctccgatt cggctcgtgg gagccggacc tcacgttcga
     2581 cgtgacggcc ggggacctcc aggaggtcga acgcatcgcg gcgaactggt atccggcgat
     2641 ccaaaacgag ccgctgtctc cgcccctgaa tctcggcggg gcgggccacg tctcggcccg
     2701 gctcacgcgc gctttctccg acccgcacgt cgagggccgc atggaggcga cggacttcgt
     2761 cctgcgcggc gtgcgcttcg gcgagacgac cgcgaacttc accgtggacc acaacgtcgc
     2821 gacgctcgcg cccttcgccg cccgcgacga cggcggcacg ctcgccctcg aggggaagat
     2881 cgggttcggc ggtgcgctgc acggccagta ccggctggac ggcctcgtcg gggatttccg
     2941 cgagtggccg ctcgaaaagg tcctggcgtt cctggacttc gacctgcccc tgtcggggcg
     3001 cgcgacggga aggctccccc tcgacggcgt cacgccggcg atccacgggc gaatcccgct
     3061 cgtcctcacg gacgcgtcga tctggggaca gaagctcgac cgcctcgaag gcgtcctcgc
     3121 gttccaggac gatcgcatcg ccgtcgaggg cgcgacgggg cgcctcggcg gcgccacggc
     3181 gagcttcggc ggctcgtacc gctacgccga cggtgcctac aagttcgacc tcgacgtgaa
     3241 ggacgttccc gcgggccgtc tcgcggctct gcaggacgcc ccggtgggcg ggctcctgac
     3301 cgggcacgcc tccggagaag gcctgatcga caagcccacg ctctccgcgg acctgcgcgt
     3361 cgcggattcg acgtacgacg gccagccgct tgcgcgggcg ggccatccgg tcgagatcca
     3421 cgcccgcgcc gaccgcggct cctggagcct gcgggccgag gccgcggaag agggcgtcct
     3481 cgagatcctc tcgcccaggg acgccgggag cgcgggccgc tggcgcgcgc gggcggagtt
     3541 cccgagcctc gcgccgctcg ccgggctcct gggtctgccc gcggatgcga aattcgacgg
     3601 ccgcctgagc gcggaggctt cgctcacgcc tggggcgtcg gctggagaac tcgacggcga
     3661 gggcgtgatc tcggccgccc agttcacggc gtggggacgg accctgacgc ttcggggcac
     3721 gacgccgctc aaggtgtcgg gaggccgcgt ctcgttcgag cgcctggcgc tcgccgaggc
     3781 ggtccggccc gaagcgccgt ccggcgtccc gacgctcgtg accctgtcgg gcagcgcgca
     3841 gttcgtcccg ccgcgagcgc tcgacgtctc ggcgatcggc tcggtggacg cttcgctgct
     3901 cgggccggcg ttcgcccccc tgaaggtcgc gggccgcatc acgctcgatg cgcggctcgg
     3961 cgggacggcg gcgtcgccgg tcgtcacggg ccgcgcggcc ctcgacggcg tggacgtcgt
     4021 gagtccggac gggggcgtgg cgttcgagtc cgcgaccggc tctctgctct tcagcgaagg
     4081 aaaggtcacg atcagcgacc tttcgctgcg gttcgcgggc gggacggtcg acctcggcgg
     4141 ctccgtcaac ctcgacggct ggaagccctc ggggatgcgc gtgtcggcgc tcgtctcgca
     4201 cgtgaaggtg tcgcccttcg acggcttccg cgggacgttc tcggggaacc tcctcatgct
     4261 cggcgacacg ctgccgcgtg cggtgcgcgg cgagctgacc ttcgaccgcg ccctctacga
     4321 ccgcgacttc tcgatcgacc tcgccgcgct tctccagcgc aagcgcgtcg cgacggtggg
     4381 cgcgaacccg acgttcctcg acccgatgac gctcgacgtc cgcctcgtgg cgccgcccga
     4441 gtccatcgag gtgcggacga acgtggcgcg gctgaaggcc tcgggcgagc tcttcgcgcg
     4501 cggcacgtgg gggcacccgc tcctcttcgg cgagatccgc gccgaggagg gcgggcggct
     4561 gacgctccag ggccagcggt acgacctcct gtcgggccgc atcctcttct ccaacccgat
     4621 ccggatcgag cccttcttcg agatggaagc gcgcggaacg gtcagcaagt accaggtcac
     4681 gttcggcctc acgggcaccg cggcgcggct cgcgacccgc ttctcgtcgg acccgcaact
     4741 ctccgaggcg cagatcatct cgctgatggc cacgggcgac gtcccgtcca cgtccgtggc
     4801 cggcgggccg atcggtcctg caccgacgtc ctcggacgag tccgtctcga aggcggcgcg
     4861 cgaactgctc gcgagcctcg cgtcggacgc cgtcacgagc cgcacgaagg agttcttccg
     4921 gctcgaccgg ttccagatcg atccggcctt ctcgaccggc tccacgttcg acgccccgcg
     4981 catcaccgtc ggaaagtccc tcgggaagga cttcaatgcg accgtctcgt tcgtcctttc
     5041 gagcaaccag cagcagatca tcacgctcga ctaccagctc tcgcggacgg cctttctcca
     5101 ggcccggctg gacgagtacg gcgtgtactc cctcgagctg cgcttcaggc agcggctgcg
     5161 gtgagagccg cgcgcggcgc cgccgccgcc ctcgtccttg cgctcggagc cgcccgggcc
     5221 ggcgccgagc cgccgcccga gggcctgctc tgggggaggc tcgtcacgac gctctcgttc
     5281 cgcggagacg gaccggtgga cgagaagcgc atggccggcc tgaccgagat cacgcccggg
     5341 aagcgcctga cggagggcgc ggtccggatc tcgctccgga atctcttcgc gacgaggcgc
     5401 ttctcggacc tttccgtcga aggggcgccg ttcggagagg gggtggcggt cgtcgtcgcc
     5461 tactcggcgg ctccacgtat cgcatcgctc gcggtcacga agggaatccc ggcgcgcggg
     5521 cgcgccctcg acgcgatcgg actccatccc ggcgacccgt gggagagcga ccgtgggccg
     5581 ggttacgaga ccgcggtccg ccgcgtgctg aaggaagaag ggtacttcga gtccgccgtc
     5641 tcggtctccg tggacgccgg cccggacgag acgagcgtca acgtgagctt cgtcgtccgg
     5701 cccggaccga aggccgtcgc ggccgcgccg gagtggaacg gaagcctggg gacgttgccc
     5761 ccgtctgcgc tcacggcgaa ggcgaagctg aaggcgggca ggccttacaa gaggaagacc
     5821 gctctggagg acgccgagag gttcgagaac gagctgcacc gcagggccta ctcgcgcgcc
     5881 gaggtgcgcc tcggagacga gcgctacgac ccggcgacgg cgtccgtctc gccccgttac
     5941 acggtcttcg tcgggccggc cgtcgtcctc gaggtgacgg gagaggacga gaaactcgtc
     6001 cgcaagcacg ccgactcgcc gtggcgcaag ggcgagccgc cggacgagga cgccgtcgag
     6061 aggctgcgcg ccgcgctcaa gcgttcgtac gaggagagcg gctacgcaaa ggcgtccgtc
     6121 aaggtcgcgt tcgacacaca gccggacaag gagatcgtcc gcttcgagat cgtgaagggc
     6181 gcccggtggt ccgtggagcg cgtggacgtc acgggcgccg tgagcctccc gccgcgcgcc
     6241 gtccagtcgg ccttcgagac gcggccccgc gggatcctcg aggccgggcg gtacgtgagc
     6301 gacgaggcgg cgcgcgaccg cgacgccctg gcgaccctct atcgggaggg cggatggcgg
     6361 gacgtgcggg tcgccgcgcc cgcggtcgtg gaaggcaggg gcgagcacac actggacgtg
     6421 acgttcgccg tggacgaggg cgtccgcacc gtcgtcgacc ggatgcgcgt cgaggggatg
     6481 cggctcctcc cggagaagga cctcgttccg cgcctctcga tcaagccggg agtcccgtat
     6541 tccgagtcgg ccgtgagcgc ggacgcggcg ctcctgcagt cgttgtacgt cgacaaggga
     6601 ttcgtcgacg cgaaggtcga ggcgaccgcg cgtttctcgg gccccgagcc tccgcagggg
     6661 aagcgggccg acgtcaccta caccgtgacc gagggcagtc cggtcctctt cggcaagacg
     6721 atcgtgcggg gaaacagccg gacgaagccc ttcatcatcg aggaccggct tgcgaacgcc
     6781 gagggagatc ctttctccct cacgaagttg ctcgacaccc agcaggcgct cgcccgcctc
     6841 ggcgtcttcg agcggatcga catcacgacg ttcgagacgg atcccgagac gatgtcccgg
     6901 agcgtcctcg tgacggtcgc cgaggcgcgg ccgtgggggc tcacgtacgc ggtcggcgcc
     6961 gagtacaacc cccagaacaa cgcgtcgctg agcgaacagc tctcgctccg gctctcgctc
     7021 gccgtgacct acaacaacct cttcggacgg gcccttgaag tcggcgtcga aggccgggcc
     7081 tcgaacaacg acccccgcat catcttcacg gcgcgggacc gatcgctctt cggcggcaag
     7141 gtcccgctgt cgttcgcgat ctacaacacg aaggacgtgc cgtcgccggc atacgacgtg
     7201 aagcgaaagg gcacgtttct ccagggcgag taccggctgt cgaagtcgct ccggaccggc
     7261 ctccgcgtcc agtacgagct cgtcgagccg tcctcggatc ccggcctcgg cgcggaccag
     7321 cgcggaaaca cggagagccg gatcgcgtcc gtgtcgagcg gcgtgacctg ggacaggcgc
     7381 gacgatccgc tgaatccgcg gaacggcttc cttctcggga cggacctcaa gtacgcgttc
     7441 ccgctcttcg ccgcggacgc gcacttcttc aagctcctct cccaggccgg cctttaccgg
     7501 ggctggggct cgacccgcgt cgcgttttcg ctgcgcggcg gcgtcatctg gaactacgcg
     7561 gcgtgcccgg acccgacggg cgagacctgc gcgcccaacc tcatcatccc cgtccccgag
     7621 cggttcttcg cgggcggcac gtcgacgcac cgcgcgttca cgcgcgacaa cctcggcatc
     7681 accccgcaga ctctcaactc ggacggtgtc ggcgtgggcg gcaacgtcct cctgatcggg
     7741 aacgccgagt ggcgcatccc ggtcgccagc ggcttcgagg tcgcgctctt cgtggacgtc
     7801 ggcaacacgt gggcggaccc gaagaacgtg agcttcggcg aagtccgtac gggcgtcggg
     7861 gtcggcctcc attacctgac cccggtcggc ccgctgcgcc tcgagtacgg catcaagctc
     7921 gaccgcaagc ccaacgagga cgcgggcgcg ttcgcattcg cggtcgggta cccgttctga
     7981 gccgggctta gaatcagcag cacatgcccc gcttcaccgt tctcgagacg tccgccctgt
     8041 tctcgaaccg cgtgaagtac gactacctct tcgagctcga gatgtggctc aaggggctcg
     8101 aacgcttctt ccagatcgac tgcctcccgc tctcggcgtt cgagcgctcg cacgcgtcgc
     8161 tcaagaacta cgccgaggag gtcgcggcgg cccgttcggg agtcgcgcac atggggctga
     8221 tcgcgacgca gctcatgggg gagggccagg aagacctcgc ctcgttcctc ctgtacctcg
     8281 acacacaggt ccagcgaccg ggggggcgcc gccggggcga cgggacgacg gagctcgcgc
     8341 gcgtcgtcga gacgctcgac gacttcacga agatcctcga cgagctcgcg aaggcgccct
     8401 tcatcccgct ccagacctat ctctcgctcg gacgggtcgt cgtcgggacg ctccggcagg
     8461 acccgaacct cggcgtcctg ttccgggaga acctccggcc cgtgctcgac cgcgagtcca
     8521 agcagtctct cgcgaaggtt ctcggcagcc tgccggaccc gtcccaccgc ggggcgctcg
     8581 cgacgctctt cgtggcgctc ttcaagggcc tgcgcgtcgc cgagcgcgtc tccgcggcgg
     8641 cggaagggcc cgccacgcgc aagcgcgcgc ttctcctctt cagcttcctg aagtccgaga
     8701 gcgattcgtt tgttgcgtac gtccggcggc ggctccggcc gcggttcgaa gacgggacga
     8761 aggaagccga aggcctcgac cgtctcgcgt ccgcgttcga ggccgagacg cgcaaggcga
     8821 tggacgtcga gctcgtgggc gtcgcgctca tgaaggaccc cgacgccgtc ctcgcccggc
     8881 tcggcgacgg ggccggcctc ttccgggacc tcttccagca gagcgtgatc ggcctcgcgg
     8941 aaacgttctc gtcgggaatc gacgcgcgct ccctcttccc ggagtaccgg gcgcgcctcg
     9001 accagagcct gcgcctgcgc gacgagctcg gccatctctc ccgcgcgacc caggacttcc
     9061 tcaagacacc ggacaagaag acgctgcgcg ccctcgtgac ggagatcgaa agcttccgcc
     9121 ggggcgggat gcggtatctg atgttcaagg actggtccct gttcgagagg ttccacgcgg
     9181 gcttctcgcg cgagcgcgtc ccgcgcgctt ttctccctgc ggcgaaccag ttcgagggct
     9241 tcctgaggac gctcttgaag gaagtcggca agcgtgcggt cctcgcggac caccccttca
     9301 ggagcccgag acccgacgcg gggtccggcg gcgcgtcccg gtacaattga cccgagggac
     9361 gcgtcctgcg tcccgtcaga gccgaaagga acaccccagt gggattcgtc tccgtcgtcg
     9421 acgccgtcgc ggcttacaag aagggccagt tcgtcatcat cgtggacgac gaggaccgcg
     9481 agaacgaggg agacctctgc ctcgccgcag agcacgtcac gcccgccgcc atcaacttca
     9541 tggcgaagga aggccgcggc ctcgtctgcg tttccctgac cgaggagcgc tgtgacgaac
     9601 tcgacctcca cccgatggtc gagcagaaca cctcgaacta cggcacggcc ttcacggttt
     9661 ccgtcgaggc gcgcggaaag acgacgacgg ggatttccgc ggccgaccgc gccgcgaccg
     9721 tcctgacgct cgtcgacccg aagtcgaggc cgcacgacct cctgaggccg gggcacacgt
     9781 tcccgttgcg ggccaagaag ggcggcgttc tcaagcgggc gggccagacg gaagcctccg
     9841 tcgacctcgc gcggatcgcg gggcttgcgc ccgcggccgt gatctgcgag atcatgaacg
     9901 aggacggcac gatggcccgg gtcccggacc tcctgctcgt ctcggaaaag cacggcattc
     9961 cgatcgtgtc ggtcgcggac atcatccggt accggatgcg gaccgagcgc ctcgtccacc
    10021 gcatcgcggc cccggtcctg ccgacgccgt tcggcgagtt ccgcgcggtc gcgttccgct
    10081 ccgacctcac gaacgaggag cacgtcgcgc tcgtgatggg aacctggacc gaggacgagc
    10141 cggtcctggt gcgcgtccat tcggcgtgcc tcacgggcga cgtcttcggc tccgggcgct
    10201 gcgactgcgg cgcccagctc cagcgcgcga tggcccagat cgccaacgaa ggcaagggcg
    10261 tgctcctcta cctgttgcag gagggtcgcg ggatcgggct cttcaacaag ctgcgggcgt
    10321 acgagctgca ggagcagggc ctcgacacgg tcgccgcgaa cgagaagctc ggcttcgcgc
    10381 cggacgtgcg cgactacggg atcgggtcgc agatcctgcg cgacctcggc gtcaagaaga
    10441 tgcgcctcat gacgaacaac ccgtccaagt acgtcgcgat cgacggctac ggcctggaga
    10501 tcgtcgagcg cgtgccgctc gagatcccgc cgacggacgg gacgcgcgac tacctcgcga
    10561 ccaagaaagc caagatgggc catctcctca agctcgtttg agcgaagaga gagaagaccg
    10621 gggattcgga tttcttagaa ggtaataggg ccgtggggtg cggccgtcag tcgcggtatc
    10681 gcttcgccaa ttccgcgggc gattcccccc ctcttaccct tctaagaatc tcccaattcc
    10741 ctctgacggt cttccacacg cctttctcga gatagcgccg cggcgaggtc tcgacgcgtg
    10801 cgcggagcag cgcgatgcga gcacgccctt ccgcctgctt cagtcgaatc gagaggtccc
    10861 ggtcctcgag gaatggccac ggccggtggc cgccgaggcg ttcgtagacc tcgcgcctga
    10921 cgaacggcgc ctggtcgccg tacgggacct tcgtgatccg cgtccggacg ttcgcccacg
    10981 cggccaccca cgcgaggcgc gggtctccgc cggaaaagcc gagccggaat gcgccccctg
    11041 ccgcgccgcg cgtgagcgcg gcgcgaacct cggtctccca gccgacaggc agcagcgtgt
    11101 cgcagtgcag gaagaggagg atttctgctg aagaggagga tcgggcggcg cgcgcgagcc
    11161 gcgcgccgcg ggggatttct tcgaaggcga agagtgcagc cccggcgtgc tgaaacgccc
    11221 cgcgggtctc ggggcggatg cgcgcatcgg ccgccacgag gagcgaaacg ggaggcccgg
    11281 cccctcttac cctttcaaag aaatcccccg gtgggggagc ttcaccggcg cccaccggaa
    11341 cgatgacggt gattgagctg gcggccaacg cgttagcatc ttggcatggc gtatgggttc
    11401 gtcctcgtcg gcgggcggtc ggtgcgcatg gggcgcgaca agacgctcct gccgtaccac
    11461 ggccagccca tggcgtacca ccaggccgcg aagctcgcgc gggtgtgcgg gcgggtcgcg
    11521 ctcgtcggca agcacccgga gctcttcacg ggctcgccgt acccgttcat cgaggacgga
    11581 gcggctccca cggccgcaat cttcggcgtt gccgcggcgc tcgcggcatc gcccgacgag
    11641 acgaacctga tcctcgcggc cgacatcccg aggatcggcg agggcttcct cgcggccctg
    11701 ctcgaggtgg cggatgcgat cccggccgac gctgtcgtgc cggtctccgg cggcgtcgcg
    11761 cagccgctct gtgcggtctg gcggcgatcc gcccatccgc ctcttctcgc gcggctggcc
    11821 gcgggcgact acgcgctcgt cggtgccctc cagcagatcc gcaccgtcct gatccccgag
    11881 gctgcgacgg ccgcgctgcc gggcgggcac ccggacaact tcctgaacgt gaacacgccg
    11941 gaggactacg agcatgccga aaaagacgcg ggcgccgcgg ctcagccacc tcgactctga
    12001 ggggcacgcc cgcatggtgg acgtctcggg aaagagcgag accgtccgct cggcccgcgc
    12061 cgtcgccgtc gtgacgctcg ggcgcgcggg atgggccgcg ctggacgcgg ccgagaaccg
    12121 caagggcgac gcgctcgcgg tcgcgcgcct cgcgggaatc caggccgcca agcggacggc
    12181 ggagtggatc cctctctgcc acccgctcgt cttcgactcg gtcgacgtcg ccgtcgaacg
    12241 cctcgcgtcg cgacgcgccg tcgccctgac ggccacggtc cgcggcacgg ggaagacggg
    12301 ctacgagatg gaggcgctgg tcgccgcgtc cgccgcagcc cttgcgctct acgacatgtg
    12361 caaggccgcg gacaagggga tcgtcatcgg gccggttgcg ctcctcgaga agaccggcgg
    12421 caagagcggc gcgtggcgcc gcggcgtcag cgtcccggca gcttccgcgc gagcgcgcgg
    12481 agggcgaacc gcaggcggtc ggcccagggc aggcggagga agttcgtctt cacgacgccg
    12541 cgtgtgaaga acgtcttgac cgagtagtcg atcgccacct ccacgaggac gggccggccg
    12601 gcatcggcga tctccttcgc gttcgagacg acggagcgga tctcgccgtc cgtcgaaatc
    12661 gcgactgcct ccgcgccgag ggcgcgggcg agcgcgagga ggtcgtggtc ggcgagcgtg
    12721 ctcgaagcct tgcggccgag ggccgtcgcc tggaactgcg cgatctgcgc gagctcccgg
    12781 tccctcagga cgaaaacggc gacgccgagt ccgttctgcg ccgcggtgag gagctcgagc
    12841 ccggtcatga ggaacgcccc gtcgcccgcg agcgcgacga ccggggaggc cggccgcgcg
    12901 agcttggcgc cgatggcggc gggcaccgcg tagcccatgc acgagaagtc cacgggcgcc
    12961 aggagccggc ccggcctctc gagacgcagg ccttcgaccg cgaggaacgt cccgttgccg
    13021 ctgtcggacg tgaagaccgt ctcggggccg aacgtcgcct ggagggcgcg caggaggagg
    13081 tgcggcgaca cgcgcccttc cgagggcctc gcgagctccg cgtccacctc cgcgcgcccg
    13141 ctgcggatgc ggtccctgag gccggcgtcc ggctcgcgcc ggtcgaggcg gggcaggagc
    13201 gccgcgacga aggcggcggc atccgcggcg acggccaggt cggtgggcac gttgcggccg
    13261 ggcacggccg ggtcgatgtc cacgtgaacg aggggccgcg gaggggtcat cccatagctg
    13321 cccgtcgcga cctcaccgaa gcggcagccg atcgcgagcg tggcgtcgca cgaggccgcg
    13381 acgtcgcggg cgaacgcggg cgcggcgtct ccgaagcccg gccacaggga cagcgggtgc
    13441 gactcgggaa agacgccctt gccctggaac gtcgtcgaca cgggcgcctc gaggcgctcg
    13501 gcgagcgcga ccaggtcggc cctcgcagcc ccgaggccga cgtagaggag gggccgcttc
    13561 gcagcccgga ggatctccgc ggcgcgttcg atgctgcgct cgggcagcgc cgcgcgcggc
    13621 tcgggagcgg gcgggacgac gtccgtctcg tgccggaaga ggtactggtc ggccggcact
    13681 tccacgaaga cggggcccgg cgtccctgaa cgcgcgatcc ggcaggcgcg gcggacggcg
    13741 tcgtagaggt ccgccccgga gggcgggagg atctgggcct tggtgaccgg gcgcgcgagc
    13801 gcgagctggt cgacgtcgtg cagctggtac gccttgcccg tgtcgcgccg gatgccgcag
    13861 ccgaggacga gcatcgggac gccgtcgagg aacgcctcgg cgattccgga catcgcgtgc
    13921 gtgaggcccg cgccggggac gacgttcacg cacgccatga cgcccgacgc gcggcacgcg
    13981 gcgtccgcca tgaaggacgc gctctgctcg tccgagacga ggacggaacg cacggactcc
    14041 gaagacgcga gcgcgtccca cagctcgatg ttgtgcgtgc cgggtatccc gaacgcgagg
    14101 gcgatccctt cgtcctcgag ggccttgacg acgagctcgg cgccggtcct cttcatcgcg
    14161 cccgcccggg ggcgccgagg agcgcaccgg cgtcttcgcg gatgcgctcg gcggcgcggt
    14221 cggcgagcgc catcacggtg aggtacgggt tgatctcgag cgagtcgggg aagaggctcg
    14281 agtcggcgac gaagagcccc ggccgcccgt ggacgcggcc ccacgagtcc gtcacggagt
    14341 cggcgggaga cgagcccatc ccgcagccgc ccatgaggtg cgccgcggag acggagatgc
    14401 ggccggtgcg gaagtggcgc tcgtcgacgc gagagtcgac cgcaccggct tccgagcgct
    14461 cgaggagcgg cgggtcggcc gacggcgcgt ggacgcgcag cgcgcccgcc gcgaagaaga
    14521 tgcgtgcggc ggcgcgcgtc gccctcacga gagacgcgac ggtttccttc gtgaaggagt
    14581 agtggacgac ggccttgccg tccgtgtcca cggagatgcg gttctcggga accgccttgt
    14641 cgcacgcaag gacgaggatc atctggaggc gcgggaaggc ccgcatgagc gtcgcgtgcg
    14701 gaacgccgaa gcccgtgagg ttcttcgccg tgatgaacgg gaagtacatg cacgtctcga
    14761 ggacgtagcc ctcttccacc gcgcggtcga ggtagaagct cttcgggtgc ccgacgtcgt
    14821 tggtgatcgg gcgctcgtgc tcggccacga ggatgtgcgc cggatggcac gtgaagcccg
    14881 cgccgaggcg ggggagcgac ggcccgaggc ccgagcgcag aagaagcgcc gtcgtcccga
    14941 tcgagccgcc tgccgcgacg atcgccttgg cgctcacggc gtactcgccg ggcgaccact
    15001 ccgagggagc gcccttttcc ccggctgccg gcgccgagac acggatcacg acgcggccct
    15061 cttccacgcg cagcgcctcg gcgcgcgtga ccgcctggac gccggcggcc agcgctgcgg
    15121 ggagctggac cacgtgcgtg ccctgcttgg cgccgttggg gcagccgagg ttgcagaggc
    15181 tcgaaccgcg gcagccgcgc aggttgagcg ggaactgctc ggccttgagg cccgccttcg
    15241 aacaaccttc gacgaagagc cggttgttgt cgttgagaag ctccggctcg agaaggtgga
    15301 ctccgttctc ggccatgaac ttccgcgagc gcgattcgac gtcggcgtgc gcgaggccgg
    15361 gcacgcgcca gctctcgatc acgcgtctcg gcgcgacgag ggacgtgccc gtgtagacga
    15421 ccgtcgagcc gccccacacg cgcccgaacg cgagcgtcat cgctccatcc gccgtcagga
    15481 atccgccgcc gtcctcgtaa agggcgtcgg ccatgtcgtc ttccttgccg gtgaactcgg
    15541 actcggcgag ccgcgcaccc tgctccacga cgaggatccg gtagccggcg cgcgcgagcg
    15601 gcgcgaggcc ctcggcgacc gtgcctccgc ccgcgccgga gccgatcacg acgacgtcgt
    15661 aatgccgggc gttcatgctc ggcgcgcctc ccacccgcgg gcgtccgcgc ggtaaccgat
    15721 cgcggcggcc gcctcgggcc gcgcgtagta gccgaggaag acgagcgttc tcaggcccca
    15781 gaagccgcgc ctgaggagga ggacgggcga atgttcgaag cccgagagga agcgcgtgcg
    15841 ctcgtccggc gagagcgccg tgaacgggcg gccccagcgc agccgcggca ggtgctcgat
    15901 cgcgcggacg aacgtaacga gctgacgctg catgcccgcc ggcttcttcg cgagggtctt
    15961 ctcgacgagc gcttcgagcg cgttccaccc cggctcgtcg agcgcggagg ccatgggcac
    16021 gaccgtgacg gcgacggcgc ggaacgtgtt ccggacggga gcgagaagca cgggggaaga
    16081 ctaccccgga ccgtggcgat atggcaccgg accggtcctg tttgggctag acttcaccgt
    16141 ctcatgggca ggaccctcgt cgacgggcac ggccggcgcg tcacctacgt ccgcatctcg
    16201 atcacggacc ggtgcaacct gcgctgcgtc tactgcatgc ccgaggacca gaagttccgc
    16261 ccgctcccgc acctcctcac gcgcgaggag atccgccggt cggcgcgtct cctgcgcgat
    16321 ttcggcgtcg aaaagattcg gatcacgggc ggcgagccga cgacccggcc cgacctcgtc
    16381 gacatcgtgc gcgacctcgg ggagatcggc tttccgggag ggatcgcgct ctcgacgaac
    16441 ggcgtcctct tcccgcgcat ggcgcacgac ctcctcgcgg cgggtctcac gaaggtgaac
    16501 gtctcgctcg acgcggccga ggccgagcat ttccgcaaga tggcgcgccg cgaccattac
    16561 gcgcccgttc gcgagtcgat cgagctcgcg cttgcgctgc cgttccactc cgtcaaggtc
    16621 aacgcggtcg tcatccgcgg gttcaacgac gaccagatcc ttccgctcgc ggccctcacg
    16681 aaagacaaga aactcggcgt ccgctacatc gagttcatgc cgttcggcga caacacctgg
    16741 aacgagagcg actggatcgg ctggcgcgag gtatgggacc ggctcgcggc cgcggggttc
    16801 gtcctgacgc ccatcgagaa gacgctcgtc gcgggacccg cggacgagta ctccgtgacg
    16861 tcgcccgccg ggagccacac gggctcgatc ggtttcatca cgccggtttc cgaccgcttc
    16921 tgcgacacgt gcaaccggat gcgcctcacg caggacggtt tcctgaaggg ctgtctcttc
    16981 ggccacgcgg acgtcgacat cctgaagccg atgcgcgccg gtgcgaacga cgaggaaatc
    17041 gcggctctga tccagcgagc cgcgaccgtc aaggaagagc ggcacccgat gttcgtcgga
    17101 atggacaagg cgatgctccc gctccacgcg cggaacatga gcgagatcgg cggctgaggc
    17161 gcccgtgcgc gtcgccgtcc tgacgatctc ggaccgttcg tatcacggcg agcgtgccga
    17221 caagacgggc cccgcggtcg ccgaggcggc ttgccgcctc ctgccgggag cctcggtcgt
    17281 cgagcagacg atcctccccg acgaaaggga cgacatcgag tggaatctca agcggatcgc
    17341 ggacaccgcg tccgcggacc tcgtcctcac ggcgggcggc accggccttt cgccgcgtga
    17401 cgtgacgcct caggcgacgc aggccatcgc cggctacgat gttccgggca tcggcgagct
    17461 gatgcgggcc gcaacgcggg cggcgctgcc ggtcgcgatt ctgtcgcggc aggtggctgc
    17521 cgtgagggag cggacgctga tcgtgaacct gccgggctcg ccgcgaggcg ccgtcgagtg
    17581 cctcgaggcc gtcgcggacg tgttgccgca cgcggttgcc accttgcgaa acgaggcagg
    17641 cgacgagcat ccggcgcgtc tcgagcgcgc ctgacagaga ttcgattgcc ctccgggtag
    17701 gcacgttttc cgttcaacaa cctcggccga gaccgccgtt ttttggcgct cgatcgtgcc
    17761 gcggcgccta aagcattgag aattcggcgc tggcgtggcg cttgctatca tttctttcgg
    17821 ttttctgtga actccatgag ggggcaatgg taagtcttcg ccgaagcatc gcaatgtcct
    17881 tgatcgggct gctgctcgct tcggcgggcc cgaatgcgct tgcccaggaa gcgcaaatca
    17941 cgaactggac cgcccctccc ttctggacgc cgggttcgat cggcaccgtg gacacccctg
    18001 aggcgggagg acgcaccgct ctggggcggc agtcgctggc cggtggcgca accccgctgc
    18061 cgttcatcgc gctcgcgccc tgccggctcg tcgacacgcg cggaaacgcc ccgctgacgg
    18121 gcgggtttct gcccgcgtcc acgatccgca gctacacgct cgtcggcgtc tgtaacgtgc
    18181 cggcgggagc cgcggcgatt tccctcaatg caacggtcgt caacgccgct ggtcccgggt
    18241 tcctcacgct ctggccggag ggcggagcgt tcccgcccgt ctccacgctg aactacgtct
    18301 cgggcggaat cgtgaccaac gcggccgtgg tgcccttgag cggcacaggg gggatttcga
    18361 ttgcgctcgg cgtctcgggc gcggacgtca ttctcgacac gaacggttac tacgcgccca
    18421 tctcggtcgt gaacagcctc aacaccctca ccggtgacgt caccctggct ccgggcgcga
    18481 acatcagcat cacgccgtcg gggaactcgc tgatgattac gtcgacagga ggcgccaccg
    18541 gacccgctgg accggccggt cccacaggcg ctgctggtcc cacgggcgcc actggaccga
    18601 ccggcgcaac aggagccacc ggcccgacag gtgccgttgg tccgacagga gccacgggtg
    18661 cgaccggcgc gaccggagcg actggtgccg ttggtccgac tgggccgact ggagcaaccg
    18721 gcgccaccgg gtcgactggt gcaactggcg cagttggtcc cactggcccg acgggagcaa
    18781 ctggagccat gggttcaaca ggtgctaccg gtgccacggg cgccacgggt tcgaccggcg
    18841 caacgggcgc cgtcggtccg actggagcga cgggcgcaac gggtgcggta ggcccgactg
    18901 gtcctgcggg tgcgacgggc gcagtgggtc cgacaggagc gactggctcg actggagcaa
    18961 ctggtgccgt tggtccgact ggtcctacgg gagctgccgg cgcgacggga gccacgggcc
    19021 cgattggagc gactggtgcc gttggtccga ctggttcgac gggcgcagtc ggtccgaccg
    19081 ggccgactgg agcaacgggc gccaccggag ccacgggttc gaccggcgca actggcgctg
    19141 ttggtcccac tggtcccacg ggagcgactg gtgcgaccgg agcaacgggc gccactggcg
    19201 ccacgggttc gaccggcgca actggtgccg ttggcccgac tgggccgacg ggtg
//
LOCUS       DAOMFY010000047        18912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_911_length_18912_cov_9.302328, whole genome shotgun sequence.
ACCESSION   DAOMFY010000047 DAOMFY010000000
VERSION     DAOMFY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18912
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_18912_cov_9.302328"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PLB01_06035"
     CDS             <1..1093
                     /locus_tag="PLB01_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQQ76894.1"
                     /translation="FRGDPSSALLEVLDPEQNHSFVDHYLDAEYDLSKVMFIATANVI
                     HPIPPALKDRMEIIVLSGYTPNEKVAIAKQFLVKKQVKENGLTPEQAVFEDGALYGLI
                     EKYTREAGVRSLEREIASVCRKIARKIIGKDVEPPVTVTADNLAGFLGKPKYRLSKKG
                     EKAEIGLTTGLAWTETGGDVLHIETTLMRGKGNLVLTGQLGDVMQESARAALSYVRTR
                     AELYGAEPDFYEKKDIHVHIPEGAIPKDGPSAGITMATSILSAVTRIPVRKDIAMTGE
                     ITLRGKVLPVGGIKEKLLAAYRAGITTIVLPKENDKDLDDLPEEIRNVMEFHLVEDVD
                     EVVRLTLEGAPSPVMPKDLAAEAGTTTPQ"
     gene            1184..1885
                     /gene="yihA"
                     /locus_tag="PLB01_06040"
     CDS             1184..1885
                     /gene="yihA"
                     /locus_tag="PLB01_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQQ76895.1"
                     /translation="MKTTFSPARSRAGSRPGGAKGVLASGAEVRLALAGHRPGECPKN
                     ALPALAVLGRSNVGKSSLLNALLGVKVARVSQTPGRTQALYWYRVGDRFDVVDCPGYG
                     YAKTGKEKRISFEGLIESLLLGTPEDAEAAAAGDRGGGATAALLLVDSRIEPQESDLS
                     MSLFLRESSVPSFVAATKWDAVKPSQRVRQLRALTAAFAAPDRPLLPVSAETGENLPE
                     LARLLRAALAPSPKE"
     gene            2000..3247
                     /gene="rho"
                     /locus_tag="PLB01_06045"
     CDS             2000..3247
                     /gene="rho"
                     /locus_tag="PLB01_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQQ76896.1"
                     /translation="MLDIRTLHNQTMPQLLKIAQELEVEGAPALRKQELIFKILQTQT
                     ERLGLIFSEGVLEVLPDGFGFLRAPEYNYLAGPDDIYVSPSQIRKFGLRTGDTISGQI
                     RPPKEGERYFALIKVEAVNFEHPDVAREKILFDNLTPLYPQERIHLETPNMSSRVLDL
                     IAPVGKGQRGLIVAPPRTGKTMLLQALANSITTNHPEVTLIVLLIDERPEEVTDMQRS
                     VKGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTTCPP
                     SGKVLSGGVDANALQKPKRFFGAARNIEEGGSLTIMATALIDTGSRMDDVIFEEFKGT
                     GNMEVHLDRKLVDRRVFPAIDINKSGTRKEELLLEKQELNRIWILRKVLNPLSTVEAM
                     ELLIERLDKTKNNAEFLGSMSAG"
     gene            3309..4118
                     /locus_tag="PLB01_06050"
     CDS             3309..4118
                     /locus_tag="PLB01_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQQ76897.1"
                     /translation="MRRERGACALLMLGVLLGAWRGDAQTANEAAELAKKLSNPVANL
                     ISVPLQSNWDYGLGTTNATKYVLNIQPVIPITLTERWNLITRTIVPYVSFGALSPVES
                     GAGGLGDTVQSFFLSPKEPTGGGWILGAGPVLLYPTATSRSTGSGKWGAGPTVVLLKQ
                     EHGWTYGVLANHLWSFAGPENRASVNSTFLQPFLSFTTATYTTIGFNTESTYDWTNDA
                     WTVPLNLTAAQLVKVGGKPVSFTLGGRYYATTPSGGPDWGLRFVVTLLFPK"
     gene            complement(4159..4743)
                     /locus_tag="PLB01_06055"
     CDS             complement(4159..4743)
                     /locus_tag="PLB01_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HQQ76898.1"
                     /translation="MPHARRLVLFGALALFAVLAVPAAAQNTAAPAAPPAPATWTFTH
                     TPAAVVKDLGAFLYPKNNQSAEQQNKDTTECLLFANDQTGINLDTFKKTGQNAEEKQA
                     AQAPKKGGAVKGAAKGAAAGAVVGEVTNDDAGKGAGYGAAAGAVAGRRAQKKGEQQAA
                     QAQKDAEAKAIADAKETVKKPFFACLDAKGYSAK"
     gene            4907..6790
                     /locus_tag="PLB01_06060"
     CDS             4907..6790
                     /locus_tag="PLB01_06060"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQQ76899.1"
                     /translation="MTLVSPRTGVALGVVTLALALASCSKPSPPAAPAAASTAPAAPV
                     AAALPPPNPENNVYFGDVHVHTGWSFDALTNGSKTTPMDAYAWAQGRRITGSGGPDLQ
                     LQTPLDFYMVADHAEYMGVFNQMSNPDSPLSKTELAKGVNSPDANVRLQTFAGILRDM
                     SAGKRDPQLSDPALARTVWAEIIKAAEANYVPGKFTTFAGFEWTSNPEKRNLHRVVVF
                     RDTKHLPDMVLSALDSDDPEVLWKWMDDQRAKGSTLFAVPHNGNASDGLMFDVVKRDG
                     KPIDAAYNKTRNANEPLYEITQIKGTSETHPDLSPKDEFAGFEQWDYTLSADFERPKR
                     RKGSFVRQALLDGMSQAAAGAGNPFQYGFIGDTDTHNAAASHEEYNFTGKFAFENNAK
                     ERLTGLPGSPAGQVQQIQEFSSGGLAGVWAPQNTREAIFDAMQRRETFGTTGPMMKVR
                     FFGGFDFAAGDVNSADFVKTAYAKGVPMGGNLKKGAGKAPTFLVMALKDPKSGNLDRI
                     QIVKGWIDSSGKQHEKVYDVVWSGDRKLGANGKLPPVGNTVDVKTATYTNTIGAAQLA
                     IGWTDPDFKPAERAFYYARVLEIPTPRWNTYDAARLNMPPLTKVPATLQERAWSSPIW
                     YTP"
     gene            complement(6798..7787)
                     /locus_tag="PLB01_06065"
     CDS             complement(6798..7787)
                     /locus_tag="PLB01_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HQQ76900.1"
                     /translation="MTANLTPQYHDAEERFKRATDHGEKLAALREMIALLPKHKGTEK
                     MLAHLRKQLSKLEDEAASAPRGGHGHRTEVGHVKREGAGQWVLLGPPNAGKSSLLAAL
                     THAHPEIGEYPFTTRAPLPGMMEFEDVKVQLVDTPAVAEGHTEPYLPNLAHDADGVLL
                     VVDVTADDGGAAALLHDLLARARVWPAARPRPADAPSFLASKPVLVLGNKSEDDDGTF
                     ATLAKEAIGEDLPFYPVSALAGAGLDALRPVLFRELRRIRIYGKEPGHKPDMDRPFVL
                     PEGATIHDFAAHVHKEIAERMKFARIWGHAKFDGQQVDRHHVLADKDVVELHA"
     gene            7862..11488
                     /locus_tag="PLB01_06070"
     CDS             7862..11488
                     /locus_tag="PLB01_06070"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HQQ76901.1"
                     /translation="MNVPEALRTPRARKVLAVAAGLLVFWAVLGFVVLPAVLRPVVER
                     KIGEALHRKAALRGLSINPFALSVTLKGLEVKDRDAASPFLSFESLYLNAEMSSVFRG
                     GPVLRAITLAKPAVTLVRNGDGTYSIQDLIEEFSKPKPKDGRPLRFSLNNIRVSGGSV
                     DFYDRPKRTRHAVRDVVIGIPFLSNIPSKVEITTQPAFEAQVNGAPFALHGKTKPFST
                     TRETTLDLNFSDVDVPFYLAYVPAEIPSKITSARLDAKLVLAFTQPVAGDPALVVSGT
                     AALRKVAVMQGREPLVAWERFDADAEAIDVFGRKAKIRSLRVTAPELWVRRHTMAEHD
                     IANALAAPGMTKTKSSSKGEEGRGAPPRPAGKPFAVDLAEATISAGVVHYDDFAFHPS
                     FHAVLTNVAAALKGFSTRAGAPAALEASAASDAGERFQSTGTLSMNPLALAGSFSAEA
                     VPLRRYTTFVDEFLPVEIDAGVLGLKTGYTFSTGKDANTTLSDLAVAIVSPRVRKKAE
                     KEPFFKAASLEMTGSSLDVAKHTFVLGGFTGTNGVLAVVREKDGNADLMELVPKPKPK
                     PDAPPPPPSDPWDVTLRKIDLRGWTVKVDDRGWDHPARYALTKADLLLENLSTARGSK
                     GALAAKFGVDGKGVAAARGPVGFRPMFADLKVDVKNVDLVPLEAYVLPNLRLYLAKGL
                     VSAGGTLALREDAAGKASVVYTGNALVANVLAVDESTKLDFLKWETFSATGMRAGYNP
                     LFLEVKQLAAVGLACDVTIEADGTVNLRRVVGAPAPRENEDDGPPPDARPAPAPAPAP
                     AAAAPGEKDARLTMPIRIDEITLQGGRIGLADFLVKPNFSATLGDLGGRVTGLSSEEG
                     TVATLDLRGSLANHSPLQVSGTINPLAAATFADVKASFRDIDLPAFTPYSGKYAGYTI
                     ASGALTIEVDYKVVNRKLNASNRFLVNQFDFGEKVESKDATKLPVKFAVSLLKDKDGV
                     IDLDLPIEGSLDDPKFRIGKIVWQVLGNIVSKAVTAPFALLGKLFGGKGDELSSVDFP
                     DGLATLDDAGKKKLDALAKALADRPALRLQATGRFSGADLEGLRNARVERKIKAQKLP
                     DLAKAGSAPASVDDVVVEAAEREGYLKKAYKKEKFPKPRTTLGFAKDLPADEMEKLML
                     VNERVGDDDLRQLALARANAVKDYLVGPAGADPARVFVLEPGTQAAAPKDKARASRVD
                     FSLK"
     gene            complement(11495..12310)
                     /gene="ppk2"
                     /locus_tag="PLB01_06075"
     CDS             complement(11495..12310)
                     /gene="ppk2"
                     /locus_tag="PLB01_06075"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HQQ76902.1"
                     /translation="MARKAKKKDENDGKLSAKKYAKELRRLQVELCHLQEWVKHKGLR
                     VIVIFEGRDGAGKGGTIRALTERVSPRVFRLVALPAPSDREKSQMYLQRYMAHFPAAG
                     EVVIFDRSWYNRAGVERVMGFCTDEQYDRFLELCPVVEKYITDGGILLIKYWLEVGNK
                     EQERRFVARITDPLRQWKLSPMDLPGRTKWYEYSLARDRMLKATDTRHAPWVLVPSDD
                     KKRARLNCIAHLLSTIPYKKVPREKIKLPKRSNKHAYDDQASLKGRRFVPEKY"
     gene            complement(12320..13756)
                     /locus_tag="PLB01_06080"
     CDS             complement(12320..13756)
                     /locus_tag="PLB01_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HQQ76903.1"
                     /translation="MKRALASLLALALAGCAMGPDYKRPATAPPENHRGATMPAVERS
                     LADLAWWDLAKDDPVLVGLLKEALEKNQDLRIAASRVEEARAISRINVVLPTVAAGAG
                     ANRQRITENGLGSAFGGQTVNTFDLSASFSWEIDLWGRLRRTNESNLARFLATEEARR
                     GVYLALVSDVAGAYYQLRALDLQLEIAHRTVGSRKDSFDLVEKRLRGGIGNRLETSQA
                     ASALAQTEIAIPQIEQAIFEQENLISLLLGRTPGPIARGRAIGEMPAVEIPAGLPSAL
                     LERRPDVRQAEANFRAANADLGVAEANLFPQLSLTGSAGFQSADLSDLLKSPSFVWNI
                     AGGIFAPIFQGGRLRRERDAAHARWDQARLAYEKAALAAFGDTASSLNAIGKTREIRE
                     ANGRNVAALREAEKTAFLRYDGGVSPYLEVLDAQRQLFSAELNFATSLRDQKLAVIRL
                     YRALGGGWNTPEAPPDGAVPAGSASSSK"
     gene            complement(13758..16943)
                     /locus_tag="PLB01_06085"
     CDS             complement(13758..16943)
                     /locus_tag="PLB01_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HQQ76904.1"
                     /translation="MSSFFVRRPIVAIVISIVMIIVGAVALKGLPIAQYPEITPPLIQ
                     VTTTFTGASAVDVEQAVATPIEQQVNGVDNMLYMKSTNANDGTLKTEVSFEVGSNLDM
                     SNVLTQNRVSQSTAQMPQSVKEYGVTVKKSLSFPLVLVSLSSPKGTWDSVFMSNYANI
                     NVVDKIKRIRGVGDTILFGGSDYAMRIWIRPDRLAKLGLTVMDLQNAIKQQNTLSPAG
                     QIGGEPAPKGTQFTYTVKTQGRLLNVEEFGDIVVRSNPDGSQVFLRDVARIELGSLLY
                     NQTGRLNGKPSAVIGVYQSPGSNALAVRDAVNKTMKEIEPSFPKDLAWTMSLDTTEAV
                     SEGISEIVHTLFEAVLLVILVVFIFLQNWRATLIPLLTVPVSLVATFAVFPLLGFSVN
                     VLSLLGLVLAIGIVVDDAIVVVEAVMHHIEHGMAPREATLQAMKEVSGPVVAIALVLS
                     AVFVPVAFMGGITGRLYQQFAVTIAISVCFSAFNALTLSPALSAMLLKPKAESKSFLD
                     PFYNWFNKVFGKFTGGYTSFAAILIRKSVRSLIFIGILVALTVGLVKSLPTGFLPDED
                     NGYFMINCQLPDAASLERTDATLKKVEQITLANPAIESVTTITGFSLLTGAMAPNTGF
                     IFVKAKPWGERKDVKERVDNTMKALNFAYYKNIPEAQVFAFAPPPITGLGSGSGFTFM
                     LQDRAGRSPAELDDMAKKFLAEAKKRPEIGRVSTVFRASVPQVYADIDRQKVLKVGVP
                     VSDVNGTLGALLGSAYINDFNRFGRVYKVFMQAEPEFRGSPKSLGLFFVRAADGSMVP
                     LDTLVKTKETSGPDFTVRFNLYRAAEISGVPAEGYSSDQALRALEETAAKVLPSGWGY
                     DWSNMSFQEKRAAGTAGAVFIMAIVFVFLILAAQYESWSLPFSVLLGTPFAAFGAFFG
                     LWAARHLMPAVYGNSYVSNVFAQIGLIMLIGLAAKNAILIVEFAKENVEKKGMPVVEA
                     ALDAAKLRFRPILMTAFAFILGVLPLVRASGAGAESRKVMGMAVFAGMLIATMLGVCL
                     VPVLFVVVEKLGGRKHAPPEPAASPAPPGGH"
     gene            complement(16948..18138)
                     /locus_tag="PLB01_06090"
     CDS             complement(16948..18138)
                     /locus_tag="PLB01_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ76905.1"
                     /translation="MSSVFAARTLRRIGMPLAVAFLLTACGKKEAVKLPAPEVYVTKA
                     VAKDVPVGMELIGQTAGSKDVEIRARVEGYLDTVNFRDGDFVKQGDLLYTIDPKPFEA
                     TVAQAKAQVATMKARVDNTQIEEARLKPLAEQQAVSRRDYDNAVAARDAALAQLDAAK
                     AQLDKAQLDLGYTRMSAPVSGLADFSRVKPGNLVGRGESTLLTTISVVDPIYFTASIT
                     EADYLRFAQRAASAGSASGAKREAELVLADGSVYDHKGRLDAVQRAVDPKTGTLAVRL
                     LFPNPDRVVRPGQYGRIRFVADVLSGAVCVPQKSVSELQGVYQVVVVGSDNKAEIRTV
                     KMGPRFGDLWAVAEGLKAGETVVVEGLQSARPGAEVVAKPIEELAKAAAAPAAPASAP
                     SAGK"
     gene            18343..>18912
                     /locus_tag="PLB01_06095"
     CDS             18343..>18912
                     /locus_tag="PLB01_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQQ76906.1"
                     /translation="MWERTFLRDLAVVLAASFPVLFVCRRLKLPQVVGFLVTGVAIGP
                     HALGWVREASRVESIAEFGVALILLFVGLEFPIRRLKALGRTSFVGGSLQMALTAAAG
                     FALARAIGAASNEAVFLGLLVSVSSTAVVLPILKQRDELAAPYGRRFLAVALFQDLAV
                     IPILLFLPALVSGSGPGPAAVAGRVVLAIA"
BASE COUNT         3038 a         6406 c         6483 g         2985 t
ORIGIN      
        1 cttccgcggc gacccgtcgt cggcgctcct cgaggtcctc gaccccgagc agaaccactc
       61 cttcgtggac cactacctcg acgccgagta cgacctctcg aaggtcatgt tcatcgcgac
      121 cgcgaacgtg atccacccga tcccgcccgc cctgaaggat cggatggaga tcatcgtgct
      181 ttcgggctac acgccgaacg agaaggtcgc gatcgcgaag cagttcctcg tgaagaagca
      241 ggtcaaggag aacggactca cgcccgagca ggccgtcttc gaggacggcg cgctctacgg
      301 cctcatcgag aagtacacgc gggaggccgg cgtccggagc ctcgagcgcg agatcgcatc
      361 cgtctgccgg aagatcgccc gcaagatcat cgggaaggac gtggagcccc ccgtgaccgt
      421 cacggccgac aacctcgccg ggttcctcgg caagccgaag taccgcctga gcaagaaggg
      481 cgaaaaggcc gagatcggcc tcacgaccgg cctcgcgtgg accgagacgg gcggagacgt
      541 cctccacatc gagacgacgc tcatgcgcgg caagggcaac ctcgtcctga cggggcagct
      601 cggcgacgtc atgcaggagt cggcgcgcgc ggcgctctcc tacgtgcgga cgcgggccga
      661 gctctacggc gccgagcccg acttctacga gaagaaggac atccacgtcc acatccccga
      721 gggcgcgatc ccgaaggacg ggccctcggc gggcatcacg atggcgacgt ccatcctctc
      781 ggccgtcacg cggatcccgg tccgcaagga catcgcgatg acgggcgaga tcacgctccg
      841 cgggaaggtc ctgcccgtgg gcggcatcaa ggagaagctc ctcgcggcct accgcgcggg
      901 gatcacgacg atcgtcctcc cgaaggagaa cgacaaggat ctcgacgatc tgccggaaga
      961 aatccggaac gtcatggagt tccacctcgt cgaggacgtg gacgaggtcg tccggctcac
     1021 gctcgagggc gcgccctcgc ccgtgatgcc gaaggacctg gcggcggaag cgggcaccac
     1081 cacgcctcag tgataagcta acccctcggg ggcggctccg ccccctctta ccttctaaga
     1141 aatctgctct tcttcccctc cccggggaat ttgattcatc ccaatgaaga cgacgttctc
     1201 tcccgcgagg tcgcgcgccg gctcacggcc cggcggcgcg aaaggtgtgc tcgcctccgg
     1261 ggccgaagtc cgcctcgccc tggccggcca ccgtccgggc gagtgcccga aaaatgccct
     1321 tccggcgctc gcggtactcg gccgctcgaa cgtcgggaag tccagcctcc tgaacgcgct
     1381 cctcggcgtg aaggtcgcgc gcgtctccca gaccccgggg cgtacccagg ccctctactg
     1441 gtaccgcgtc ggcgaccgct tcgacgtcgt ggactgcccg ggatacgggt acgcaaagac
     1501 ggggaaagag aagagaattt cctttgaagg gttgatcgag agtctgctgc tcggtacgcc
     1561 cgaggatgcc gaggcggcgg cggccggtga tcgcggcggt ggcgccacgg ccgctctcct
     1621 cctcgtcgac agccgcatcg agccgcagga gagcgacctc tccatgtccc tctttcttag
     1681 agaatcttcg gtcccctctt tcgtcgccgc gacgaaatgg gacgccgtga agccgtcgca
     1741 gcgcgtgcgc cagctgcgcg ctctcacggc cgcctttgcc gcccccgacc gccccctcct
     1801 gcccgtctcg gccgagaccg gcgaaaacct gcccgagctc gcgcgcctcc tgcgcgcggc
     1861 gctcgcaccc tccccaaagg agtaacccat gccccgacgc aaggtccccg gcgccgatta
     1921 cgccgtccgc gacatcgacc cccgcgagct cccggccgac gtagaagagc agccggccgc
     1981 ggccgccgcc gtcgagccga tgctcgacat ccggacgctc cacaaccaga cgatgccgca
     2041 gctcctcaag atcgcccagg agctcgaggt cgagggcgcg ccggccctgc gcaagcagga
     2101 gctgatcttc aagatcctcc agacgcagac ggagcggctc ggcctcatct tctccgaggg
     2161 cgtcctggag gtgctcccgg acgggttcgg cttcctgcgc gcccccgagt acaactatct
     2221 ggccggcccg gacgacatct acgtctcgcc ctcgcagatc cgcaagttcg gtctgcgcac
     2281 gggcgacacg atctccggcc agatccgccc gcccaaggaa ggggagcggt acttcgccct
     2341 catcaaggtc gaggccgtca acttcgagca ccccgacgtc gcgcgcgaga agatcctctt
     2401 cgacaatctg acgccgctct acccgcagga gcgtatccac ctcgagacgc cgaacatgtc
     2461 ctcgcgcgtc ctcgacctca tcgctcccgt cggcaagggc cagcgcggcc tcatcgtcgc
     2521 gccgccgcgt acgggcaaga cgatgctcct ccaggcgctc gcgaactcga tcacgacgaa
     2581 ccaccccgag gtcacgctca tcgtcctcct catcgacgag cggcccgagg aagtcacgga
     2641 catgcagcgc tcggtcaagg gtgaggtcat ctcctcgaca ttcgacgaac ccgcgacgcg
     2701 gcacgtgcag gtcgccgaga tggtcatcga gaaggcgaag cgcctcgtcg agcacaagcg
     2761 cgacgtcgtg atcctcctcg actcgatcac gcgcctcgcg cgcgcctaca acacgacgtg
     2821 cccgccctcg ggcaaggtcc tgtcgggcgg cgtggacgcg aacgcgctcc agaagccgaa
     2881 gcggttcttc ggggctgcgc gcaacatcga ggagggcggc tcgctcacga tcatggcgac
     2941 ggccctcatc gacacgggat cgcggatgga cgacgtgatc ttcgaggagt tcaaggggac
     3001 cggcaacatg gaggtccacc tcgaccgcaa gctcgtcgac cgccgcgtct tcccggccat
     3061 cgacatcaac aagtcgggca cgcgcaagga agagctgctg ctcgagaagc aggagctcaa
     3121 ccggatctgg atcctccgca aggtcctcaa cccgctctcg acggtcgagg ccatggagct
     3181 cctcatcgag cgcctggaca agacgaagaa caacgcggag ttcctcgggt cgatgtcggc
     3241 cgggtgatcg cgcccgatcc ggggcgtcga ggttgtcgag acgatcaacg aggggaggac
     3301 cattcaccgt gcgacgtgaa cgtggagcgt gcgcgcttct catgctgggc gttcttctcg
     3361 gggcgtggcg tggtgatgcg cagaccgcca atgaggccgc cgagctcgcg aagaagctct
     3421 cgaaccccgt ggcgaacctc atcagtgtgc cgcttcagag caactgggac tacggcctcg
     3481 gtacgacgaa cgcgacgaag tacgtcctca atatccagcc cgtcatcccg atcacgctga
     3541 ccgagaggtg gaatctcatc acgcgcacga tcgtgccgta cgtctcgttc ggtgcgctct
     3601 cgccggtgga gtcgggcgcc ggcggcctcg gcgacacggt acagagcttt ttcctgtcgc
     3661 cgaaggagcc gacgggcggc ggctggatcc tcggagccgg gccggtgctg ctctatccca
     3721 cggccacgag ccggtcgacg ggctcgggca agtggggcgc gggcccgacg gtcgtcctac
     3781 tcaagcagga gcacggctgg acgtacggcg tcctcgcaaa tcacctctgg tcgttcgcgg
     3841 gcccggagaa tcgcgcgagc gtgaattcaa cgttcttgca gccgttcctg tctttcacga
     3901 ccgcgacgta cacgacgatc ggtttcaaca ccgagtccac ctacgactgg acgaatgacg
     3961 cctggaccgt cccgctcaac ctcacggccg cccaactcgt gaaggtgggc ggcaagccgg
     4021 tgagcttcac gctgggcggt cggtactacg cgacgacgcc gtcgggcggg cccgactggg
     4081 gcctgcgctt cgtcgtgacg ctcctgttcc cgaagtagga taggaaacgc ggccggcgct
     4141 tccgccggcc gcaccggttc acttcgcgct gtagcccttc gcgtcgaggc aggcgaagaa
     4201 cggctttttc accgtctcct tcgcgtcggc gatcgccttc gcctcggcgt ccttctgagc
     4261 ctgcgcggcc tgctgctcgc ccttcttctg cgcccgccgg cccgcgaccg cgcccgcggc
     4321 ggccccatag cccgcgcctt tgcccgcgtc gtcgttcgtg acctcgccga cgacggcgcc
     4381 ggcggcggcg cccttcgcgg cgcccttcac ggctccgccc ttcttcggag cctgtgcggc
     4441 ctgcttctcc tcggcgttct ggcccgtctt cttgaacgtg tcgaggttga tccccgtctg
     4501 gtcgttcgcg aagaggaggc actccgtcgt gtccttgttc tgctgctcgg cggactggtt
     4561 gttcttcggg tagaggaacg cgccgaggtc cttgaccacc gcggcgggcg tgtgcgtgaa
     4621 cgtccaggtg gccggcgccg gcggagcggc cggggcggcg gtgttctggg ccgccgccgg
     4681 gacggcgagg acggcgaaga gggcgagggc gccgaagagg acgagacggc gagcgtgcgg
     4741 cattcggtct ccttttcctt cgaggtgtct tacgggcggg cggatctttt cgcggattgc
     4801 ggccgctcgc aaccgggatt cgggccgccg gctggaggtt cttcacccgg gaggatccct
     4861 gggttaggct aagaacgaat ctcagcctga accgagagga gcctccatga cgctcgtttc
     4921 cccgaggacc ggagtcgccc tgggcgtcgt cacccttgcg ctggccttgg cctcgtgctc
     4981 gaagccgtcg ccgccggccg cgcccgcggc ggcttccacg gcgcctgccg cgccggttgc
     5041 cgccgcgttg ccgccgccga accccgagaa caacgtctat ttcggcgacg tccacgtcca
     5101 cacgggctgg tccttcgacg cgctgacgaa cggctccaag acgactccga tggacgccta
     5161 cgcctgggcg caaggccggc ggatcaccgg cagcggcggc ccggacctcc agctccagac
     5221 ccctttggac ttctacatgg tcgccgacca cgccgagtac atgggcgtgt tcaaccagat
     5281 gtcgaacccc gacagcccgc tcagcaagac ggagctcgcc aagggcgtga actctccgga
     5341 cgccaacgtg aggctccaga cgttcgccgg catcctgcgc gacatgagcg ccggcaaacg
     5401 ggacccgcag ctgagcgacc cggcgctggc gcgcacggtc tgggccgaga tcatcaaggc
     5461 ggccgaggcg aactacgtgc ccggaaagtt cacgacgttc gccggcttcg aatggacgtc
     5521 gaaccccgag aagcggaacc tccatcgcgt cgtcgtcttc cgcgacacga aacacctgcc
     5581 ggacatggtg ctgtcggcgc tcgattccga cgacccggag gtgttgtgga aatggatgga
     5641 cgatcagcgc gcgaagggct cgacgctgtt cgccgttccg cacaacggca acgcgagcga
     5701 cggcctcatg ttcgacgtcg tgaagcgcga cggcaagccg atcgacgcgg cctacaacaa
     5761 gacccggaac gcgaacgagc cgctctacga aatcacccag atcaagggga cgtcggaaac
     5821 gcatccggac ctgtcgccga aggacgaatt cgccggcttc gagcagtggg actacacgct
     5881 ctcggcggac ttcgagcggc cgaagcgccg caagggcagc ttcgtgcgcc aggcgcttct
     5941 cgacggcatg agccaggcgg cggccggcgc cggcaatccc ttccagtacg gcttcatcgg
     6001 cgacaccgat acgcacaacg cggcagcgag ccacgaggag tacaacttca cgggcaagtt
     6061 cgcgttcgag aacaacgcga aggagcgcct gaccggccta ccgggctcgc cggcgggcca
     6121 ggtccagcag atccaggagt tcagctcggg agggctggcc ggcgtctggg ccccgcagaa
     6181 cacgcgcgag gcgatcttcg acgcgatgca gcgccgtgag acgttcggaa ccacgggccc
     6241 gatgatgaag gtgcgcttct tcggcggctt cgacttcgcg gccggcgacg tgaacagtgc
     6301 cgacttcgtg aaaacggcct atgccaaggg cgttccgatg ggcggaaacc tgaagaaggg
     6361 ggccggcaag gcaccgacgt tcctcgtcat ggcgctcaag gaccccaaga gcggaaacct
     6421 ggaccgcatc cagatcgtca agggctggat cgactcgagc ggcaaacagc acgagaaggt
     6481 gtatgacgtc gtctggtccg gcgaccgcaa gctcggcgcg aacgggaagc tgcccccggt
     6541 cggcaatacg gtcgacgtga agaccgcgac gtacacgaac acgatcggcg ccgcgcagct
     6601 cgcgatcggc tggaccgatc ccgatttcaa gcccgccgaa cgcgcgttct attacgcccg
     6661 ggtcctcgag attcccactc cgcgctggaa cacctatgac gcggcccggc tgaacatgcc
     6721 gccgctgacg aaggtgccgg cgaccctcca ggagcgcgcc tggagttcgc cgatctggta
     6781 cacgccgtag cggttcctca ggcgtgcagc tccaccacgt ccttgtcggc gaggacgtgg
     6841 tggcggtcca cctgctggcc gtcgaacttg gcgtggcccc agatgcgggc gaacttcatc
     6901 cgttcggcga tctccttgtg gacgtgcgcg gcgaagtcgt ggatcgtcgc gccctcgggg
     6961 aggacgaacg gccggtccat gtccggcttg tgccccggct ccttcccgta gatccggatt
     7021 cgcctgagct cgcggaagag gacgggacgc agggcgtcca gccccgcgcc cgcgagggcc
     7081 gagacggggt agaaggggag atcctccccg atcgcctctt tcgcgagcgt cgcgaacgtg
     7141 ccgtcgtcgt cctcgctctt gttcccgagg acgaggaccg gcttgctggc gaggaaggac
     7201 ggcgcgtcgg ccgggcgcgg ccgggcggcg ggccacaccc gggcgcgtgc gaggaggtcg
     7261 tggaggagcg cggcggcgcc gccgtcgtcg gccgtgacgt ccacgacgag gaggacgccg
     7321 tcggcgtcgt gcgcgaggtt cggaaggtac ggctccgtgt ggccctcggc gaccgccggc
     7381 gtgtccacga gctgcacctt cacgtcctcg aactccatca ttccgggaag cggggcgcgc
     7441 gtcgtgaacg ggtactcgcc gatctcgggg tgcgcgtgcg tgagggcggc gaggagcgag
     7501 gacttgcccg cgttcggcgg gccgaggagc acccactggc ctgccccctc gcgcttcacg
     7561 tgccccacct ccgtgcggtg cccgtggccg ccgcgcggcg ccgaggcggc ctcgtcctcg
     7621 agcttggaga gctgcttgcg caggtgcgcg agcatcttct cggtgccctt gtgcttcggg
     7681 aggagggcga tcatctcgcg cagggccgcg agcttctccc cgtggtccgt cgcccgtttg
     7741 aatctctctt cggcgtcgtg gtactggggc gtcaggttcg cggtcacggc tcaaggatag
     7801 gagccgtgtc cccgggcgcg cctgattcac ccgcatcgtt taggatcgcc gccatccgcc
     7861 catgaacgtg cccgaggccc tccggacgcc ccgagcgagg aaggttctcg ccgtcgccgc
     7921 cggccttctc gtcttctggg cggtcctcgg cttcgtcgtc ctgccggcgg tcctccggcc
     7981 cgtcgtcgag cggaagatcg gggaggcgct gcaccgcaag gcggcgctgc gtggactgtc
     8041 gatcaatccg ttcgcgctct cggtcacgct gaagggcctc gaggtgaagg accgcgacgc
     8101 ggcgagcccg ttcctctcct tcgagagcct gtacctgaac gccgagatgt cgtccgtctt
     8161 ccgcggcggg cccgtcctcc gggcgatcac gctcgcgaag cccgccgtca ccctcgtgcg
     8221 gaacggcgac ggaacgtaca gcatccagga cctgatcgag gaattctcga agccgaagcc
     8281 gaaggacggc aggcccctcc gtttctcgct caacaacatc cgggtctcgg gcggcagcgt
     8341 cgacttctac gaccgtccga agaggacgag gcacgctgtc cgcgacgtcg tgatcggaat
     8401 cccgttcctc tcgaacatcc cgtccaaggt cgagatcacg acgcagcccg ccttcgaggc
     8461 gcaggtgaac ggcgctccgt tcgccctgca cgggaagacg aagccgttca gcacgacgcg
     8521 cgagacgacg ctcgacctga acttctcgga cgtcgacgtc ccgttctacc tcgcgtacgt
     8581 tcccgccgag atcccgtcga agatcacgtc ggcacggctc gacgcgaagc tcgtcctcgc
     8641 gttcacgcag ccggtcgcgg gagaccccgc cctcgtcgtc tcgggcacgg cggcgctccg
     8701 aaaagtcgcc gtcatgcagg ggagagagcc tctcgtcgcc tgggagcggt tcgacgcgga
     8761 cgccgaggcg atcgacgtct tcgggcgcaa ggcgaagatc cggagcctga gggtcacggc
     8821 gccggagctc tgggtccgcc gccacacgat ggccgagcac gacatcgcga acgcgctcgc
     8881 ggcaccgggg atgacgaaga cgaagagttc ttcgaaaggt gaagaggggc ggggcgcgcc
     8941 gccgcgaccg gccggcaagc cgttcgccgt cgacctcgcc gaggcgacca tttccgctgg
     9001 cgtcgtccac tacgacgatt tcgcctttca cccgtctttc catgcggtcc tgacgaacgt
     9061 cgccgccgcg ctgaagggct tctcgacgag ggcgggcgcg cccgcggccc tcgaagcgtc
     9121 ggcggcctcg gatgccggag agcgctttca gagcacggga accctttcga tgaatcctct
     9181 cgccctcgcg gggagcttct cggccgaagc ggtgcctctg cgtcgctaca cgacgttcgt
     9241 cgacgagttt ctccccgtcg agatcgacgc gggcgtcctc gggctgaaga ccggctacac
     9301 gttctcgacc gggaaggacg cgaacacgac gctttcggac ctcgccgtgg cgatcgtctc
     9361 gccgcgcgtg aggaagaagg ccgagaagga gccgttcttc aaagccgcgt cgctcgagat
     9421 gaccggctcg tccctcgacg tcgcgaagca cacgttcgtc ctcggaggct tcacgggaac
     9481 gaacggcgtc ctcgcggtcg tccgcgagaa ggacggcaac gccgacctca tggagctcgt
     9541 cccgaagccg aagccgaagc cggacgcgcc gcccccgccg ccgtccgacc cgtgggacgt
     9601 cacgcttcgg aagatcgacc tccgcggctg gaccgtgaag gtcgacgacc gcgggtggga
     9661 tcatccggcg cgctacgcgc tcacaaaagc cgatctcctc ctcgagaacc tctccacggc
     9721 cagaggctcg aagggagcgc tcgccgcgaa gttcggcgtg gacgggaagg gcgtcgcggc
     9781 cgcgagaggt cccgtcggtt tccggcccat gttcgcggac ctgaaggtgg acgtgaaaaa
     9841 cgtcgacctc gtgccgctcg aggcctacgt cctcccgaac ctccggctct acctcgcgaa
     9901 gggcctcgtg agcgccggcg ggacgctggc gctgcgcgag gacgcagccg gcaaggccag
     9961 cgtcgtctac acggggaacg ccctcgtcgc gaacgtcctc gccgtggacg agtcgacgaa
    10021 gctggacttc ctcaagtggg agacgttctc ggcgacggga atgcgggccg gctacaaccc
    10081 gctgttcctc gaggtgaagc agctcgcggc ggtcggcctc gcgtgcgacg tcacaatcga
    10141 ggccgacggg accgtgaacc tacggagggt cgtcggggcg cccgcccccc gggagaacga
    10201 ggacgatggg ccgccgcccg acgcgaggcc ggctccggcg cccgcccctg ccccggcggc
    10261 ggccgcgccg ggcgagaagg acgcgcggct cacgatgccg atccggatcg acgagatcac
    10321 gctccagggc ggacggatcg gcctcgcgga cttcctcgtc aagccgaact tctccgcgac
    10381 gctcggagac ctcggcggac gcgtgaccgg gctttcgtcc gaggagggga ccgtcgcgac
    10441 gctcgacctg cgcggcagcc tcgcgaatca ctcgccgcta caggtctcgg gcacgatcaa
    10501 cccgctcgcg gccgcgacgt tcgcggacgt caaggcgagc ttccgcgaca tcgacctgcc
    10561 ggccttcacg ccgtactcgg gtaaatacgc gggctacacg atcgcgagcg gcgccctgac
    10621 gatcgaagtc gactacaagg tcgtgaaccg gaagctgaac gcgtcgaacc gcttcctcgt
    10681 caaccagttc gacttcggcg agaaggtcga gagcaaggac gcgacgaagc tccccgtgaa
    10741 gttcgcggtc tcgctcctga aggacaagga cggcgtcatc gacctcgacc ttccgatcga
    10801 gggctcgctc gacgacccga agttccggat cgggaagatc gtgtggcagg tccttgggaa
    10861 catcgtctca aaggcggtga cggcgccttt cgcgctcctc gggaagctct tcggcggcaa
    10921 gggcgacgag ctctcgtccg tggacttccc ggacggcctc gcgacgctgg acgacgcggg
    10981 gaagaagaag ctcgacgcgc tcgcgaaggc gcttgcggat cgacccgccc tcaggctgca
    11041 ggcgaccggg cgcttctccg gcgcggacct cgaagggctg agaaacgccc gtgtcgagcg
    11101 gaagatcaag gcccagaaac tgccggacct cgcgaaggcg gggagcgcgc cggcgagcgt
    11161 ggacgacgtc gtcgtcgagg cggccgagcg cgaggggtac ctcaagaagg cctacaagaa
    11221 ggagaagttc ccgaagccgc ggacgacgct cgggttcgcg aaggaccttc ccgccgacga
    11281 gatggagaag ctcatgctcg tgaacgagcg ggtcggggac gacgacctgc gtcagctcgc
    11341 gctggcccgg gcgaacgccg tgaaggacta tctcgtcggg ccggccggcg cggacccggc
    11401 gcgcgtcttc gtcctcgagc ccggcacgca ggccgcggcg cctaaggaca aggcccgcgc
    11461 gagccgcgtc gacttctcgc tgaagtaggc ggcctcagta cttctcgggc acgaaacgcc
    11521 ggcccttgag cgaggcctgg tcgtcgtagg cgtgcttgtt cgagcgcttc gggagcttga
    11581 tcttctcgcg gggcaccttc ttgtacggaa tcgtgctcag gaggtgcgcg atgcagttca
    11641 ggcgcgcgcg cttcttgtcg tcggacggga cgaggaccca gggcgcgtgc cgggtgtcgg
    11701 tcgccttgag catccggtcg cgcgcaagcg agtactcgta ccacttcgtg cgccccggca
    11761 ggtccatggg gctcagcttc cactggcgca gggggtccgt gatgcgcgca acgaagcggc
    11821 gctcctgctc cttgttgccg acctcgagcc agtacttgat gaggaggatg cctccgtccg
    11881 tgatgtactt ctcgacgacg gggcagagct cgaggaagcg gtcgtactgc tcgtccgtgc
    11941 agaaccccat gacgcgctcg acgccggcgc ggttgtacca gctccggtcg aagatcacga
    12001 cctcgcccgc ggcggggaag tgcgccatgt agcgctggag gtacatctgg ctcttctcgc
    12061 ggtccgacgg ggcgggcagc gcgacgagcc ggaagacgcg cgggctgacg cgctcggtga
    12121 gggcgcggat cgtgccgccc ttgccggcgc cgtcgcgccc ctcgaagatg acgatgaccc
    12181 gcagcccctt gtgcttgacc cactcctgga ggtggcagag ctcgacctgc aggcgacgga
    12241 gctccttcgc gtacttcttc gcggagagct tcccgtcgtt ctcgtctttc ttcttcgcct
    12301 tcctcgccat ttcggctccc tacttcgagg aggacgcgga ccccgcgggc acggcgccgt
    12361 ccggcggtgc ctcgggcgtg ttccagccgc cgccgagcgc ccggtagagg cggatgacgg
    12421 cgagcttctg gtcgcggagg gacgtcgcga agttgagctc ggccgagaag agctggcgct
    12481 gggcgtcgag gacctcgagg taaggcgaca cgccgccgtc gtacctcagg aacgccgtct
    12541 tctcggcttc gcgaagcgcg gcgacgttgc gcccgttcgc ctctcgaatc tcgcgggtct
    12601 tgccgatcgc gttcaggctg ctcgccgtgt cgccgaaagc ggcgagggcg gccttctcgt
    12661 aggcgagccg ggcctggtcc catcgcgcgt gcgcagcgtc tcgctcgcgg cggaggcggc
    12721 caccctggaa gatcggcgca aagatgccgc ccgcgatgtt ccagacgaac gaaggcgact
    12781 tgaggagatc ggagaggtcc gcgctctgga agccggcgct cccggtgagg gagagctgcg
    12841 ggaagaggtt cgcctccgcg acgccgaggt cggcgttcgc ggcccggaag ttcgcttcgg
    12901 cctggcggac gtcgggccgc cgctcgagga gggccgacgg aaggcccgcg gggatctcga
    12961 cggcgggcat ctcgccgatc gccctgccgc gcgcgatggg cccgggtgtc cgtccgagga
    13021 ggagcgagat caggttttcc tgctcgaaga tcgcctgctc gatctggggg atcgcgatct
    13081 cggtctgtgc gagggcggac gcggcctggc tcgtctcgag cctgttgccg atgccgccgc
    13141 gaaggcgctt ctcgacgagg tcgaagctgt ccttccggct cccgaccgtg cggtgcgcga
    13201 tctcgagctg gagatcgaga gcgcgcagct ggtagtacgc gcccgcgacg tccgacacga
    13261 gggccagata aacgccccgg cgggcctcct cggtcgcgag gaagcgggcg aggttcgatt
    13321 cgttcgtccg gcggagccgg ccccagaggt cgatctccca cgagaacgac gccgagaggt
    13381 cgaacgtgtt gacggtctgc ccgccgaagg cgctgccgag gccgttctcc gtgatgcgct
    13441 ggcggttcgc gccggcgccg gcggcgacgg tcggcaagac cacgttgatc cgcgagatcg
    13501 cgcgggcttc ctcgacgcgg gacgcggcga tcctgaggtc ctggttcttc tcgagggctt
    13561 ccttgagcag gcccacgagc acggggtcgt ccttggcgag atcccaccag gcgagatccg
    13621 cgagggaacg ctcgacggcg ggcatcgtgg cgccgcggtg gttctccggc ggagccgtcg
    13681 cgggacgctt gtagtcgggg cccatggcgc agcccgcgag ggcgagcgcg agaagagagg
    13741 cgagggcgcg tttcatctca gtgacctccc ggcggtgccg gcgacgccgc cggctccggc
    13801 ggcgcgtgct tccttccgcc cagcttctcg acgacgacga agaggacggg cacgaggcag
    13861 acgccgagca tcgtcgcgat gagcatcccg gcgaagacgg ccatgcccat gaccttgcgc
    13921 gactcggctc cggcgccgga cgcgcgcacg agcgggagga cgccgaggat gaacgcgaaa
    13981 gccgtcatga ggatcggcct gaagcggagc ttcgcggcgt cgagggcggc ctcgacgacg
    14041 ggcatgccct tcttctcgac gttctccttg gcgaactcga cgatgaggat cgcgttcttg
    14101 gccgcgaggc cgatgagcat gatcaggccg atctgcgcga agacgttgct cacgtagctg
    14161 ttcccgtaca cagccggcat gaggtggcgc gcggcccaca ggccgaagaa cgcgccgaac
    14221 gccgcgaacg gcgtcccgag gagcacgctg aagggcagcg accagctctc gtactgcgcc
    14281 gcgaggatca ggaagacgaa cacgatcgcc atgatgaaga cggcgcccgc ggtgcccgcg
    14341 gccctcttct cctggaagga catgttcgac cagtcgtagc cccagcccga ggggaggacc
    14401 ttcgcggccg tctcctcgag ggccctgagg gcctggtcgg aggagtagcc ctcggccggg
    14461 acgccggaga tctcggcggc gcggtacagg ttgaagcgca ccgtgaagtc ggggccggag
    14521 gtctccttcg tcttcacgag cgtgtcgagc ggcaccatgc tcccgtccgc ggcgcggacg
    14581 aagaagaggc cgagggactt cggggagccg cgaaactccg gctcggcctg catgaacacc
    14641 ttgtacacgc ggccgaaccg gttgaagtcg ttgatgtacg cggacccgag gagggcgccg
    14701 agcgtcccgt tcacgtcgct gaccgggacg ccgaccttga ggaccttctg ccggtcgatg
    14761 tccgcgtaca cctgcgggac ggacgcacgg aagaccgtcg acacgcggcc gatctcgggc
    14821 cgcttcttcg cctcggcaag gaacttcttc gccatgtcgt cgagttcggc ggggctgcgg
    14881 cccgcgcgat cctggagcat gaacgtgaag cccgagccgc tcccgaggcc cgtgatgggc
    14941 ggcggcgcga acgcgaagac ctgcgcctcg gggatgttct tgtagtacgc gaagttcagc
    15001 gccttcatgg tgttgtcgac gcgttctttg acgtccttgc gctcgcccca gggcttcgcc
    15061 ttcacgaaga tgaagcccgt gttgggggcc atcgcgcccg tgaggaggct gaagcccgtg
    15121 atcgtcgtga cgctctcgat ggcggggttc gcgagcgtga tctgctcgac cttcttgagc
    15181 gtcgcgtccg tccgctcgag cgaggccgcg tccggaagct ggcagttgat catgaagtag
    15241 ccgttgtcct cgtcgggcag gaagcccgtc gggaggctct tcacgaggcc gaccgtgagc
    15301 gcgacgagga tcccgatgaa gatcaggctg cggacgctct tgcggatgag gatcgccgcg
    15361 aaggacgtgt agccgcccgt gaacttcccg aacaccttgt tgaaccagtt gtagaacggg
    15421 tcgaggaagg acttcgactc ggccttcggc ttcaggagca ttgccgagag cgccggagag
    15481 agcgtcaggg cgttgaacgc cgagaagcag accgagatcg cgatcgtgac cgcgaactgc
    15541 tggtagaggc ggcccgtgat gccgcccatg aacgcgaccg ggacgaacac ggccgagagg
    15601 acgagggcga tcgcgacgac cggcccggag acctccttca tggcctgcag cgtcgcctcg
    15661 cggggcgcca tcccgtgctc gatgtggtgc atgacggcct cgacgacgac gatcgcgtcg
    15721 tcgacgacga tcccgatcgc gaggacgagc ccgaggagcg agaggacgtt cacggagaac
    15781 ccgaggagcg ggaagaccgc gaacgtcgcg acgagggaga ccgggaccgt gaggagggga
    15841 atcagcgtcg cccgccagtt ctgcagaaag atgaacacga cgaggatgac gaggaggacg
    15901 gcctcgaaca gtgtgtggac gatctcgctg atgccttcgg agaccgcttc cgtcgtgtcg
    15961 agggacatcg tccacgcgag gtccttcggg aacgagggct cgatctcctt catcgtcttg
    16021 ttgacggcgt cgcgcacggc gagcgcgttc gaaccgggcg actggtagac gccgatgacg
    16081 gcggacggct tgccgttgag gcgccccgtc tggttgtaca ggagcgagcc gagctcgatg
    16141 cgcgccacgt cacgcaggaa gacctgcgag ccgtcggggt tcgaccggac cacgatgtcg
    16201 ccgaattcct cgacgttcag gaggcggccc tgcgtcttga ccgtgtaggt gaactgggtg
    16261 cctttcggcg cgggctcgcc gccgatctgg ccggcggggg acagcgtgtt ctgctgcttg
    16321 atcgcgttct ggaggtccat cacggtcagg ccgagcttcg cgagccggtc cggccggatc
    16381 cagatccgca tcgcgtagtc cgacccgccg aacaggatcg tgtcgcccac gccccggatg
    16441 cgcttgatct tgtcgacgac gttgatgttc gcgtagttcg acatgaagac gctgtcccac
    16501 gtccccttcg gcgaggagag ggacacgagg acgaggggga acgagagcga cttcttgacc
    16561 gtgacgccgt attccttgac ggactgcggc atctgggcgg tcgactgcga gacgcggttc
    16621 tgcgtcagga cgttcgacat gtcgaggttc gagccgacct cgaacgacac ctcggtcttg
    16681 agcgtgccgt cgttcgcgtt cgtcgacttc atgtagagca tgttgtcgac gccgttgacc
    16741 tgctgctcga tcggcgtcgc gacggcctgc tcgacgtcca cggcgctcgc gcccgtgaac
    16801 gtcgtcgtga cctggatgag gggcggcgtg atctcggggt actgggcaat cggcaggccc
    16861 ttcagggcga ccgcgcccac gatgatcatc acgatcgaga tgacgatcgc gacgatcggc
    16921 cggcggacga agaagctgga catggggtta cttccccgcg gaaggggccg acgcgggggc
    16981 tgcgggcgcg gcggcggcct tcgcgagctc ctcgatgggc ttggcgacga cctcggcgcc
    17041 gggccgcgcc gactggaggc cctcgacgac aaccgtctcg ccggccttca ggccctcggc
    17101 gacggcccag agatcgccga accgcggccc catcttcacg gtgcggatct cggccttgtt
    17161 gtccgagccc acgacgacga cctggtagac gccctggagc tcggagacgg acttctgcgg
    17221 cacgcacacg gcgccggaaa ggacgtccgc gacgaaccgg atccgcccgt actgcccggg
    17281 gcggacgacg cggtcggggt tcgggaagag aaggcggacg gcgagggtgc cggttttcgg
    17341 atcgacggcg cgctgaacgg cgtcgagccg gcccttgtgg tcgtagaccg acccgtcggc
    17401 gaggacgagc tcggcctcgc gcttcgcgcc ggacgcggat cccgcggacg cggcccgctg
    17461 ggcgaacctc agatagtccg cttcggtgat ggacgccgtg aagtagatcg ggtccacgac
    17521 cgagatggtc gtcaggagcg tggactcgcc gcggcccacg aggttgccgg gcttgacgcg
    17581 cgagaagtcc gcgaggccgg agacgggcgc cgacatgcgc gtgtacccga ggtcgagctg
    17641 ggccttgtcc agctgcgctt tcgcggcgtc gagctgggcc agtgccgcgt cgcgcgcggc
    17701 caccgcgttg tcgtagtcgc gccgggagac ggcctgctgc tcggccagcg gcttcaggcg
    17761 cgcctcctcg atctgggtgt tgtcgacgcg ggccttcatc gtggcgacct gcgccttcgc
    17821 ctgcgcgacg gtcgcctcga acggcttcgg gtcgatcgtg tagaggaggt cgccctgctt
    17881 cacgaagtcg ccgtcgcgga agttcacggt gtcgaggtag ccctcgacgc gagcacggat
    17941 ctcgacgtcc ttgcttccgg ccgtctgacc gatgagctcc attccgaccg ggacgtcctt
    18001 ggcgacggcc ttcgtgacgt agacctcggg cgccgggagc ttcacggctt ccttcttgcc
    18061 gcaggcggtg agcaggaacg ccacggccag aggcatcccg atccggcgaa gcgtgcgcgc
    18121 ggcgaaaaca ctcgacatga attcctccaa cgaaggggac attggcgtgg atcctcgcaa
    18181 tttccctgcc cggagacaaa aactttccgt cgccgaacgg ccaccattta ggccttgcgg
    18241 cctttccagc gggacgacat gtcgccgggg gtggcgaaat atcaccacac aatttcgaac
    18301 ggcgcggccc ctcaagacgc gacgcgttcc tatactggcc gcatgtggga gcggacgttc
    18361 ctgcgggacc tcgcggtcgt cctcgccgcc tcgttccccg tcctcttcgt ctgccggcgg
    18421 ctgaagcttc cgcaggtcgt cggcttcctc gtcacgggcg tcgcgatagg cccccacgcg
    18481 ctcggctggg tgcgcgaggc gagccgcgtc gagtccatcg cggagttcgg cgtcgcgctc
    18541 atccttctgt tcgtcggact cgagtttccg atccggcggc tcaaggcgct cggccggacg
    18601 tccttcgtcg gcggctctct ccagatggcg ctgaccgcgg cggcggggtt cgccctcgcg
    18661 cgcgccatcg gggccgcctc gaacgaggcc gtcttcctcg gcctcctcgt ctccgtctcg
    18721 tccaccgccg tcgtcctgcc gatcctcaag cagcgcgacg agctcgcggc gccctacggc
    18781 cgccgtttcc tcgcggtcgc gctcttccag gacctcgccg tgatcccgat cctgctgttc
    18841 ctccccgcgc tcgtctccgg aagcgggccc gggcccgccg ccgtcgcggg ccgcgtcgtc
    18901 ctcgcgatcg cg
//
LOCUS       DAOMFY010000048        18526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_938_length_18526_cov_7.721888, whole genome shotgun sequence.
ACCESSION   DAOMFY010000048 DAOMFY010000000
VERSION     DAOMFY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_18526_cov_7.721888"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="PLB01_06100"
     CDS             complement(<1..1867)
                     /locus_tag="PLB01_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HQQ76907.1"
                     /translation="MYRIVRRQQFSDTTFLWDVHAPDVAKAAQPGHFVMVRLREGGER
                     IPLTVADFDREWGTITMVVQAVGKTTREMLQDFAEGDTFPDFVGPLGLPSHVHKAGHV
                     VLVGGGLGVAPVFPQLRAFKEAGNRITGIIGFRSKDLVFWEQKFKEYCDDLIVCTDDG
                     SYGRPGFVTAALKDVLEKEKVDEIVAIGPLPMMRACCDTSRPFGVKTMVSLNAIMVDG
                     TGMCGSCRVTVDGKVKFACVEGPDFDGHLVDFKELAFRQSRFKTQEVEAQKDFDKVCS
                     YEEQLFELEKRNWKKLKELPPHATKMPERDHVERSGNFLEVNLGYRMVDALQEAERCI
                     QCVRPTCIEGCPVSIDIPRFIRHILVRDLAGALSVIQETNLFASICGRVCPQESQCEA
                     QCILVKGKMESVAIGRLERFVGDNAPAFVWSKPGNAGTLGKVAIVGSGPAGLACAGDL
                     AKAGAEVTIYEALHVIGGVLRYGIPSFRLPRTTIEREVKNLEALGVKFETNKVIGKTF
                     TIPQLQTEMGYDAVFIGTGAGAPSFLGIPGEFAGSVYSANEFLTRVNLMGGDRFPYED
                     TPVTPGRRVVVLGAGNTAMDCLRVSRRLGAAEVKCVYRRTEAEAPARIEEIRHAKE"
     gene            complement(1938..2849)
                     /locus_tag="PLB01_06105"
     CDS             complement(1938..2849)
                     /locus_tag="PLB01_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQQ76908.1"
                     /translation="MSASLAAAPSAFIGIPTQRPTSWWLETLKKRAERMYDEHLDLSK
                     PVTFESDEERLACIKFFNAAYRAEESGLAGAHKLAEEVRAWDPELAECLILYGNEEGW
                     HKELLDEFLAHIGSGPTEMGRMVKGFYTSYAKAERMETIMLTNLMFETIGATTYRLSL
                     RNVKQPLVRQVLTILTRDESFHVPLNVHFIREILKHHPERSRTRLKAIYQVVFHSLVL
                     MSLGSRKVARDFDRITWKELTKAYVENLGRLFVNEPDLDFSPPRHLLAAFGMSRAGLR
                     ETEGPSAVSIAAAEAAARREDVKVDAL"
     gene            complement(2938..3138)
                     /locus_tag="PLB01_06110"
     CDS             complement(2938..3138)
                     /locus_tag="PLB01_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76909.1"
                     /translation="MKPDATSADESVPFDETAVTRGRHKCYFCDAEKSDEWKFLAKLN
                     TFICRACLNYIEGTGTAERKAG"
     gene            complement(3149..3559)
                     /locus_tag="PLB01_06115"
     CDS             complement(3149..3559)
                     /locus_tag="PLB01_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76910.1"
                     /translation="MLNAQLPKEEPAGDVTLSLTLHAPHPILVSNTDEPGKVLLHLDI
                     RNKAQQPWEGSTSTGPLLELVLLDSEGRERARQTRQCRMLAYPTRLEPGRGFNLPLYV
                     PLPGLSSSGEDFRIEIRMVPGRELLTGHLRVQPR"
     gene            complement(3826..5082)
                     /locus_tag="PLB01_06120"
     CDS             complement(3826..5082)
                     /locus_tag="PLB01_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ76911.1"
                     /translation="MKRDEIFEALVDSDIAPAVPREEFGRTLPFVRTRVPLEDIYRLS
                     VEAEKPAELVLKAARSGPDELAAAMRALDGKPYRGFALLYAAQKGTALLGIDAKRALA
                     LAKAVEEEASSLVAANREARATTPSPRQAVQAEAHLLESQALLIMGDPTAARTAVDSA
                     RPLFVEAGDLGFGAALCDYFEGEAATFGRDYAAAEELIRSALKTFEEFGQDHFLARSE
                     AAIGNILATQGSQVEALEYFERAVMHFDPDKDARALTMTLNNRATSLAQIGRFDDARA
                     TYAKALTLALRNDFVTHLRYIRTGLAELDFLRGQYARALRAFREIAAESATNGSATDV
                     LFSRLYVAECLGRTGQFDAMAAEIEALRHDRRQTVFSPSPALGELFVCLDQGTIDADL
                     VAHVREYMQDEENGVERAYQPLRLVG"
     gene            complement(5079..5393)
                     /locus_tag="PLB01_06125"
     CDS             complement(5079..5393)
                     /locus_tag="PLB01_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76912.1"
                     /translation="MQTKVEGLAPLEGWDRRPKPSAPELELPARPVWDWAELNAKFRP
                     RLVAAGVIRFGLSEDDCEAAAQRVFLKALAMNPRVRDPKAFLRAAFLEQCFRLLGQRD
                     EP"
     gene            5519..8269
                     /gene="ppdK"
                     /locus_tag="PLB01_06130"
     CDS             5519..8269
                     /gene="ppdK"
                     /locus_tag="PLB01_06130"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQQ76913.1"
                     /translation="MATTKSTALAGLKHVYAFGAGTAEGKGDQKNLLGGKGANLAEMA
                     RLGLPVPPGFTITTEVCTHYYANNQSYPSGLRDEVAEHLAGVEKALGRKFGDPKNPLT
                     VSVRSGARASMPGMMDTILNLGLNDRTVEGLAQVSGNPRFAWDCYRRFVAMYGDVVLN
                     LKPLDKRERDPFEVILEKKKIAYNVKLDTELSVSALQELVRDFKAEIRYRTGQTFPDD
                     PMEQLWGAIGAVFGSWMNERAIVYRHLNGIPESWGTAVNVQSMVFGNLGEDCGTGVAF
                     TRDPSTGENIFYGEYLINAQGEDVVAGVRTPQKIATLDAKMPEIYKQLLGIRKTLEDH
                     YGDMQDIEFTVERGKLWMLQTRTGKRTGFAEVRIAVEMVEEKRITSEQAVLRVNPDSL
                     NQLLRPIFDPAAKNEALAGGRLLAKGLNAGPGAATGRVVFTAHDAFEWAERGEKVLLV
                     REETSPEDIKGMNAAEGILTARGGMTSHAALVARQMGKVCIVGCEVLRIEHQTGQMIV
                     TQKSGDTKMVQEGDWVSIDGSTGEVLEGRIATKPSEVLQVLLDKSLDPAKSEVYRRFA
                     TLMEWADKARRLKVRANADQPDQALNAVAFGAEGIGLCRTEHMFFGGERITAVREMIL
                     ADKTEDREKALDKILPMQREDFIGLFRAMGPRPVTIRTLDPPLHEFLPHGEKERESVA
                     RQTGVDPRLIARKVADLSEMNPMLGHRGCRLGITFPEITKMQTRAILEAACRVKKEGI
                     EVKPEIMIPLVSHVHELADQAKVVRDVAKKVFEAEGVTVDFLVGTMIELPRAAVTADE
                     IAREAEFFSFGTNDLTQTTFGLSRDDAGKFLPDYVRREILPSDPFERLDQGGVGKLVK
                     MGTELGRGTRPGLKVGICGEHGGDPSSVEFFHRTGLDYVSCSPFRIPIARLAAAQAAL
                     RK"
     gene            complement(8270..8914)
                     /locus_tag="PLB01_06135"
     CDS             complement(8270..8914)
                     /locus_tag="PLB01_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ76914.1"
                     /translation="MKIAFAALALVLTLPAPAADDLRIEPVKAALKAEKWDAAVEAGE
                     KLVREAPESAEAHLWLGRAYGQKALKSSLFSQMGWAKKCKASFEKAVALDPKNVDARV
                     DLLQYYANAPGIAGGGMDKARAQQKALDGIDPVRGAQMSGFILLKEKKPAEAEAEYRR
                     AVSLAPENGSAHWRLGRVLERGGKKDDARASYKEALRLDPTLEGAKRDLERLGG"
     gene            8970..10439
                     /locus_tag="PLB01_06140"
     CDS             8970..10439
                     /locus_tag="PLB01_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HQQ76915.1"
                     /translation="MNDRPRRHRSDLHEIARRAMREKGLTPDFPKSALEQLGAITEPA
                     PARADGLRDLRSLLWASIDNDDSRDLDQLSVAEALPNGEIRVLVAIADVDALVKKGTP
                     LDERAAHNTTSVYTAGGVFPMLPEKLSTDLTSLNPDVDRLAVVIEYVVDGGGAPHGTD
                     VYRAVVRNKAKLAYDSVDAWRDGRGPMPEAMAKVPGVDAQIRLQETAAQKLRARRYEE
                     GALDLETIEARAVFDGETITDLRVDQQNEPRQLIEDFMIAANGVTARFLEDKGYPSLR
                     RVVRSPERWLRIVTVASEHGFSLPPEPDAKALETFLKEQRRRDPMRFPDLSLVIVKLM
                     GAGEYVLEVPGRTPIGHFGLAVRDYTHSTAPNRRYPDVITQRILKAALAGRPSPYGNG
                     ELASLARHCTDQEDAANKVERLVRKAAAALLLQNRVGERFNGIVTGASDKGTWVRIFA
                     PPVEGKVVRGDEGLEVGERVRVKLLSTDVEHGFIDFARA"
     gene            complement(10417..11313)
                     /locus_tag="PLB01_06145"
     CDS             complement(10417..11313)
                     /locus_tag="PLB01_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007982816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ76916.1"
                     /translation="MTEAWTHREGRVNGIRLHWVEQGAGPLVVPLHGFPEFWYAWRHQ
                     IPALAAAGFRAVAPDLRGYNLSEKPPGVRAYRIEALLGDLEGLVALLAGEQGESRAHV
                     VGHDWGGAFAWYAPLFIPERLLSLTLLNAPHPLAFRRELRSSPAQRKKSSYVFLFQLP
                     WLPERRIRAGDFAILGKMLRRDPVTPGAFPDEDVRLYTEALAQPGALTSAVNYYRAAL
                     RYPPRARGRTWPQGLKTLLIWGERDRYLGPGLLEGLDRWVPNLTIERIPDASHWVQAD
                     APARVNEFLIRFLAGITPARSR"
     gene            complement(11310..12494)
                     /locus_tag="PLB01_06150"
     CDS             complement(11310..12494)
                     /locus_tag="PLB01_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ76917.1"
                     /translation="MNPWRGLSGLPRALWVLAGASLVNRSGTMFLPFLVLYLTSERGF
                     TPGAAGLVFAVFGAGSLAIAPVAGRLADRIGAVRLMRIALMASGALLLLVPFARGMPA
                     ITVLTLLIALTGEMFRPASLSVVSHLAAPDQRKAAFALQRLAVNLGMSVGPAAGGFLA
                     AVSWPALFIVDGVTSLLAAATLIFFFPRNALPAAEPSSGAAASRREGLRDPALVFFLV
                     ALVPVCIVFFQHESSMSVFLVRDVGLAVWIFGLLHTVNTLLIVFVEVPLNLAMAHWPH
                     RRSLVLGSVLVAAGFGALAWARSLPAVVATVVVWTFGEMILVPSMSSWVADVAPPDKR
                     GAYMGLYTMAFSFALVLGGPLGTTLLEHGGGRTLWLTMLALGLLSAALFTRVKEPGRA
                     AA"
     gene            12583..15195
                     /gene="xdh"
                     /locus_tag="PLB01_06155"
     CDS             12583..15195
                     /gene="xdh"
                     /locus_tag="PLB01_06155"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HQQ76918.1"
                     /translation="MHVELTLNGAARGFDVAADASLLELLRDHAGVTSPKDGCSPQGQ
                     CGACLVLVEGHPKTACAVPARTAAGKSVLTLEGVPEAERHVYGEAFARTAGVQCGFCI
                     PGIALRMKSVLDANPDPTDEEIRKKLDPHLCRCTGYVKIVDAFHAAARLRRGEALEPA
                     DDSGRVGTSLDRYEAEELALGARPYVADMAFPGMLHGALALSPHARARVVAVDTSRAA
                     RHPGVMAIVTAKDVPGQRFYGLIHPDWPGFVAEGEETHYVGDVLAAVAAVDARTARAA
                     AALVDVTYEVLPPVTDPDEALRDGAPRVGPEGNLLSRSVVTLGDADAALAASAHVVEG
                     TWQTQRIEHLYIEPEACLAAPGDTAGQNPKARLTLYTQGQGIFDDRRQVAGFLGLPEE
                     EVHVVLVPNGGAFGGKEDMSVQAQTALLSIVTGRPVRLVLSREESMRLHPKRHPIRMH
                     YRVGCDAEGRLTAVRARMLGDSGAYASVGGKVLERAAGHAAGPYRCPNLDVEALAVYT
                     NNPPCGAMRGFGANQAHFAMEGALDMLAEKAGVDRWEIRWRNALEAGDDWSCGQTLTA
                     SVGLKKTLLAVKKVWDASGGRAGIACGIKNTGIGNGMKEWGRARLVVGSDGSVSIHNG
                     YTEMGQGLLTVLIQCACETTGLPPSVFRAKVDTRFPMEVGQTTGSRATYLGGRAVVDA
                     AKKLKADLDSGKTLADLAGTVYVGETLVDDTTKLGHEPPPGKPMKLHSAFSFATQVVV
                     LDEKGRVAKVVAAHDVGRAINPKLCEGQIEGSVHMGLGYALTEEMVCKDGMPTSFRIR
                     DVGVLRAQDMPEVDVILVEDPQPDGPFGAKGVGEIGLVPTAPAVAGALFAFDGVRRFT
                     LPMKDSAAARALKR"
     gene            15200..16459
                     /locus_tag="PLB01_06160"
     CDS             15200..16459
                     /locus_tag="PLB01_06160"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQQ76919.1"
                     /translation="MSPLLPKVTGLRCVLCAAVYAHGEVEYTCPRCGIEGILDVEYDH
                     DLVARTFGREALAANRDLSLWRYRALLPVPDDAAALPPPRLQVGFTPVVESPVLAKEL
                     GLGAFFVKDDGRNPTASFKDRASSVGVLRARWLGRNEITCSSTGNAASSLAGFAAEAG
                     MNAFIFVPATAPEAKVAQLLVYGAKVFLVEGPYEDAFRVCQAAVEEFGWYNRNCAINP
                     YLVEGKKTCGLEIAEQMRERLPDVVTVALGDGCTTAGIWKGLLEMKTHGVVDRLPRIL
                     AVQAEGAAPLAMTFARGDERIDPVDAATLADSINVGAPRNGIKALRAVRASGGAIVTA
                     RDEAILAWIPRLARATGVFAEPTGVAALAGLEAALEKGLVRRGERVLHVATGNGLKDT
                     RGAMRSVGPPTRIPPDLDAVRRALEKK"
     gene            16456..17811
                     /locus_tag="PLB01_06165"
     CDS             16456..17811
                     /locus_tag="PLB01_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQQ76920.1"
                     /translation="MIHGPTYAEMREPGLLPAELRKRAQTAAREAPLDPVNLFNITWH
                     DEGGRVRALRLPEALTGVPAPIVLLYAKDFPTGAHKVGATYSVLMEHQLAGDVTPGTS
                     TLVWPSTGNYGIGGSWVGPRMGYESVVILPEEMSAERFEKIAAYGARAIKTPGSESNV
                     KEIYDECKRLRADPKIRVMNQFEEMANYRWHYNVTGAAAAEAVEALGAGRPAAFVSAM
                     GSSGTIAAGDLLKARFGTRIVGLEPIQCPTISRNGYGAHAIEGIGDKHVTWIHNVMNM
                     DAVMAIDDQECLLGLQLVAEPEGRDFLAETGGLGEAAHRLSTLLGVSGVCNILGAIKT
                     AKWLGLGKDDAVVTVATDGLDRYPSVLRRLEKTTGALTREKAEAHLESIFHGQKLDWI
                     SEGTSESRERWHNLKYFTWVEQQGKTVAELDAQRSGNWWAHEAGLAGDVDRRLREVRG
                     W"
     gene            17808..>18526
                     /locus_tag="PLB01_06170"
     CDS             17808..>18526
                     /locus_tag="PLB01_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ76921.1"
                     /translation="MTESAAGETGAGALAITGGTVVTLDRPSVERRDVVLGTGARRTL
                     DASGCLVLPGLAIAHTHLYSALACGMPGPGEPPRSFREILEKVWWRLDAALDEKSLEA
                     SADLALLDAVLSGVTAVIDHHESPSFIGDSLDVLARASRSSGVKAALCYGATDRHGAD
                     GARAGLAECERAIRAGLPAMVGLHAGFTVSDATLASAADLAKRTGRWLHVHAAEDRCD
                     AGSFERLEKAGRHFAARQIGRL"
BASE COUNT         2920 a         6307 c         6680 g         2619 t
ORIGIN      
        1 cctccttggc gtggcggatc tcctcgatgc gcgcgggagc ctcggcctcg gtgcgccggt
       61 agacgcactt gacctccgcg gcgccgaggc ggcgcgagac gcgcaggcag tccatcgccg
      121 tgttgccggc gccgaggacg acgacgcggc ggcccggcgt cacgggcgtg tcctcgtacg
      181 ggaagcggtc gccgcccatg aggttcacgc gcgtgaggaa ttcgttcgcg ctgtagaccg
      241 agcccgcgaa ctcgccggga atcccgagga agctgggcgc gcccgcgccc gtcccgatga
      301 agacggcgtc gtagcccatc tccgtctgga gctgcgggat cgtgaacgtc ttgccgatga
      361 ccttgttcgt ctcgaacttc acgccgaggg cctcgaggtt cttgacctcg cgctcgatcg
      421 tcgtgcgcgg caggcggaag ctcgggattc cgtagcgcag gacgccgccg atgacgtgca
      481 gcgcctcgta gatcgtgacc tcggcgcccg ccttcgcgag gtcgcccgcg caggcgaggc
      541 ccgccgggcc cgagcccacg atcgcgacct tgccgagcgt tcccgcgttc ccgggcttcg
      601 accagacgaa ggccggggcg ttgtcgccga cgaagcgctc gaggcgcccg atcgcgacgg
      661 actccatctt gcccttaacg aggatgcact gcgcctcgca ctggctctcc tgcgggcaca
      721 cgcgtccgca gatcgacgcg aagaggttcg tctcctggat cacggagagg gcccccgcga
      781 ggtcgcgcac gaggatgtgg cggatgaagc gggggatgtc gatcgagacg gggcagccct
      841 cgatgcacgt cggccgcacg cactggatgc agcgctcggc ctcctgcagc gcgtccacca
      901 tccggtaccc gaggttcacc tcgaggaagt tcccggagcg ctcgacgtgg tcgcgctcgg
      961 gcatcttcgt ggcgtggggc ggaagttcct tgagcttctt ccagttgcgc ttctcgagct
     1021 cgaagagctg ctcttcgtac gagcagacct tgtcgaagtc cttctgggcc tcgacctcct
     1081 gcgtcttgaa gcggctctgc cggaaggcga gctccttgaa gtcgaccaga tggccgtcga
     1141 agtccggacc ctcgacgcac gcgaacttca cctttccgtc caccgtgacg cggcacgacc
     1201 cgcacatgcc ggtgccgtcc accatgatcg cgttcaggct caccatcgtc ttgacgccga
     1261 acggccggct cgtgtcgcag caggcgcgca tcatcgggag cggcccgatc gcgacgatct
     1321 cgtcgacctt ctccttttcg aggacgtcct tgagcgcggc cgtcacgaag ccggggcgcc
     1381 cgtacgagcc gtcgtccgtg cagacgatca ggtcgtcgca gtactccttg aacttctgct
     1441 cccagaagac gaggtccttg gagcggaagc cgatgatccc ggtgatccgg ttgccggcct
     1501 ccttgaaggc gcgcagctgc gggaaaacgg gcgcgacgcc gagcccgccg cccacgagga
     1561 cgacgtggcc cgccttgtgg acgtgggagg ggaggccgag gggcccgacg aagtcgggga
     1621 acgtgtcgcc ctcggcgaag tcttggagca tctcgcgcgt ggtcttgccg acggcctgga
     1681 cgaccatcgt gatggtcccc cactcgcggt cgaagtccgc cacggtcagc gggatgcgct
     1741 cgccgccctc gcgcaggcgg accatcacga agtggcccgg ctgggccgcc ttcgcgacgt
     1801 cgggcgcgtg aacgtcccag aggaaggtcg tgtccgagaa ctgctgccgg cggacgatcc
     1861 tgtacatgga ccgggaggct agtggaggtg gtccccggca ggccatggtg caaatggcct
     1921 actaagaacg cgtctcgtta cagcgcgtcg accttcacgt cttcccgccg cgcggcggcc
     1981 tcggcggccg cgatcgagac ggcgctcggg ccctccgtct ccctcagccc cgcccgggac
     2041 atcccgaagg cagccaggag gtgccgcggc ggggagaagt cgaggtcggg ctcgttcacg
     2101 aagaggcggc cgaggttctc gacgtaggcc ttcgtgagct ccttccaggt gatccggtcg
     2161 aagtcccgcg cgaccttccg gctcccgagc gacatgagga cgagcgagtg gaagaccacc
     2221 tggtagatcg ccttcaggcg cgtccggctc cgctcggggt ggtgcttcag gatctcccgg
     2281 atgaagtgga cgttcagcgg cacgtggaag gactcgtcgc gggtgaggat cgtgaggacc
     2341 tgccgcacga gcggctgctt cacgttgcgg agcgagagcc ggtacgtcgt cgcgccgatc
     2401 gtctcgaaca tgaggttcgt gagcatgatc gtttccatgc gctcggcctt cgcgtacgac
     2461 gtgtagaacc ccttcaccat gcgccccatc tcggtcgggc cgctgccgat gtgcgcgagg
     2521 aactcgtcga ggagctcctt gtgccagccc tcctcgttgc cgtagaggat gaggcactcg
     2581 gcgagctcgg gatcccacgc gcggacttct tcggcgagct tgtgcgcgcc ggcgaggccc
     2641 gactcttcgg cgcggtacgc cgcgttgaag aacttgatgc acgcgaggcg ctcctcgtcg
     2701 gactcgaacg tgacgggctt cgacaggtcg aggtgctcgt cgtacatccg ctcggcgcgc
     2761 ttcttcagcg tctcgagcca ccaggacgtc gggcgctgcg tcgggatgcc gatgaaggcg
     2821 gaaggggcgg cggcaaggga cgcgctcacg gcgtgcggat gttgccgaaa agatgggcgg
     2881 gaggtcaaac ggatgcggcc gtggacgccg gaacgggacg aacaacgaag actcggctca
     2941 gcccgccttg cgttccgctg tgcccgtccc ctcgatgtag ttcaggcagg ctctgcagat
     3001 gaaggtgttc agcttcgcga ggaacttcca ctcgtcggat ttctccgcgt cgcagaagta
     3061 gcacttgtgg cggccgcggg tcacggccgt ctcgtcgaag ggaacgcttt cgtccgcgga
     3121 ggtcgcgtcg ggtttcatga cgtcctcctc agcgcggctg gacgcgcagg tggccggtga
     3181 ggagctcgcg gccggggacc atgcggatct cgatgcggaa gtcctcgccc gaagaagaga
     3241 gcccgggcag gggaacgtag agggggaggt tgaaccccct gccgggctcc aggcgggtgg
     3301 gataagcgag catgcggcac tgccgcgtct ggcgtgcgcg ctcgcggccc tcggagtcca
     3361 ggaggaccag ctcgaggagg ggacccgtgc tggtgctccc ctcccacggc tgctgggcct
     3421 tgttccggat atcgaggtgc agcagaacct tgcccggctc gtccgtgttc gagacgagga
     3481 tcgggtgcgg tgcgtgaagc gtcagcgaca gggtgacatc accggccggc tcttccttcg
     3541 gcagttgtgc gttcaacatg tcgtgactcc gttcgtgagg aatctcgaga gaccgttcag
     3601 ccctggatcg gtccgattct gaagaaatcg atgagcgaga aaagaaagtc catatgtcct
     3661 cctctgtcct tcgtaccccg cggcaggaca actctcctgc cttcactcac taagacaggc
     3721 gggacacgac cctttgaccc ccctggcggt caaaaaggcg tcggccgccc gaaggcggcc
     3781 gcgaagatgc tcagaaaaag gggatgaaag cggctctgtg cgggatcaac ccacgaggcg
     3841 caggggctgg tacgcgcgct cgacgccgtt ctcctcgtcc tgcatgtact cgcggacgtg
     3901 ggcgacgagg tcggcgtcga tcgtgccctg gtcgaggcag acgaacagct cgccgagagc
     3961 gggcgacggg ctgaagaccg tctgcctgcg gtcgtggcgg agcgcttcga tttctgccgc
     4021 catcgcgtcg aactgtccgg tccggccgag gcactcggcg acgtagagac gcgagaagag
     4081 tacgtcggta gcggaaccgt tggtggcgga ctccgccgca atctcgcgga acgcgcgaag
     4141 agcgcgcgcg tactgaccgc gcaggaagtc gagctccgca aggccggtcc gaatgtagcg
     4201 aaggtgagtg acgaagtcgt tgcgaagagc gagagtgaga gcctttgcgt aggtggctcg
     4261 ggcgtcgtca aaacgcccga tctgggcaag agacgtggcc cggttgttca gtgtcatcgt
     4321 gagcgcgcgc gcgtccttgt ccgggtcgaa gtgcatgacg gcccgctcga aatactccag
     4381 cgcttccact tggctgccct gggtcgccag gatgttcccg atcgccgctt ccgacctcgc
     4441 gaggaaatgg tcctggccga attcttcgaa ggtcttcaga gcggaccgga tcagctcttc
     4501 ggccgccgcg tagtcgcggc cgaacgtggc ggcttcacct tcgaagtagt cgcagagcgc
     4561 ggcaccgaag ccgaggtcgc ctgcttcgac gaagagcggc cttgcggagt ccacggccgt
     4621 tctcgcagcg gtcgggtcac ccatgatgag aagggcctgc gattcgagga gatgagcctc
     4681 ggcctgcacg gcctgccgtg gcgacggcgt cgtggcgcgc gcttcgcggt tggccgcgac
     4741 taaagacgat gcttcttctt ccacggcctt cgcgagggcc agggcgcgct tcgcgtcgat
     4801 gccgaggaga gccgtcccct tctgcgccgc gtacagcagc gcgaagccgc ggtagggctt
     4861 gccgtcgagc gcgcgcatcg cggcggcgag ctcgtcgggt ccggagcgcg cggccttgag
     4921 gacgagctcg gcgggcttct cggcctcgac cgagaggcgg tagatgtctt ccagggggac
     4981 gcgcgtcctc acgaacggca gcgtccgccc gaactcctcg cgcgggaccg cgggcgcgat
     5041 gtccgagtcc acgagcgctt cgaaaatctc gtcgcgcttc atggttcgtc cctctggccg
     5101 aggaggcgga agcactgctc gaggaacgcg gccctcagga acgccttcgg gtcgcggacg
     5161 cgcgggttca tcgcgagcgc cttcaggaac acgcgctgcg cggcggcctc gcagtcgtcc
     5221 tcggagaggc cgaagcggat gacgccggcg gcgacgaggc gcggccggaa cttcgcgttg
     5281 agctcggccc agtcccagac ggggcgcgcc ggcagctcca gctccggcgc ggacggcttg
     5341 ggacgccgat cccacccttc gaggggcgcg aggccttcga ccttcgtctg catcaatgtt
     5401 cgtccgcgaa cagcgcctta tgctaaaccc ggattccggc gcgggtcaaa ggtgcgactt
     5461 tgtcgcaacg ggcgctccct cgggaggccc gaaagacccg gcgggcgagg gcggcggtat
     5521 ggcgacgacg aagtcgacgg cactggcggg tctcaagcac gtttacgcct tcggggccgg
     5581 cacggccgag ggcaagggcg accagaagaa cctcctcggc ggcaaggggg cgaacctcgc
     5641 cgagatggcc cggctcggcc tcccggtgcc tcctggcttc acgatcacga ccgaggtctg
     5701 cacccactac tacgccaaca accagagcta ccccagtggt ttgcgcgacg aggtggccga
     5761 gcacctggca ggcgtggaga aggcgctggg ccggaagttc ggcgacccga aaaacccgct
     5821 gaccgtgtcc gtgcggtccg gcgcccgcgc ctcgatgccg gggatgatgg acacgatcct
     5881 gaacctcggc ctgaacgacc ggacggtcga aggcttggcg caagtctcgg ggaatccgcg
     5941 gttcgcgtgg gactgctacc gccggttcgt ggccatgtac ggcgacgtcg tcctgaacct
     6001 caagcccctt gacaagcgcg agcgcgaccc cttcgaagtg atcctcgaga agaagaagat
     6061 cgcctacaac gtcaagctcg acacggagct ttccgtctcg gcgctgcagg agctcgtgcg
     6121 cgacttcaag gccgagatcc gctaccgcac cggccagacg ttccccgacg acccgatgga
     6181 gcagctctgg ggcgccatcg gcgccgtgtt cggctcgtgg atgaacgagc gcgcgatcgt
     6241 ctaccggcac ctcaacggca tccccgagag ctggggcacg gccgtgaacg tgcagtcgat
     6301 ggtgttcggc aacctcggcg aggactgcgg caccggcgtc gccttcaccc gcgacccgtc
     6361 cacgggcgag aacatcttct acggcgagta cctcatcaac gcgcagggcg aggacgtcgt
     6421 ggcgggcgtg aggacgccgc agaagatcgc gacgctcgac gccaagatgc ccgagatcta
     6481 caagcagctc ctcggcatcc gcaagacgct cgaggaccac tacggcgaca tgcaggacat
     6541 cgagttcacg gtcgagcgcg gcaagctctg gatgctgcag acgcggacgg gcaagcgcac
     6601 gggcttcgcc gaggtgcgca tcgccgtcga gatggtcgag gagaagcgca tcacgtccga
     6661 gcaggccgtc ctgcgcgtga accccgactc gctgaaccag ctcctgcgcc cgatcttcga
     6721 cccggccgcc aagaacgaag cgctcgccgg gggccgcctc ctcgccaagg ggctcaacgc
     6781 gggccccggc gcggcgacgg gccgcgtcgt cttcacggcg cacgacgcct tcgagtgggc
     6841 cgagcgcggc gagaaggtgc tcctcgtgcg cgaggagacg agccccgagg acatcaaggg
     6901 catgaacgcg gccgagggga tcctcacggc gcgcggcggc atgacgtcgc acgcggcgct
     6961 cgtcgcgcgt cagatgggaa aggtctgcat cgtcgggtgc gaggtcctcc gcatcgagca
     7021 ccagacgggc cagatgatcg tcacgcagaa gtcgggcgac acgaagatgg tccaggaggg
     7081 cgactgggtg tcgatcgacg ggtcgaccgg cgaggtcctc gagggccgca tcgcgacgaa
     7141 gccgtcggag gtcctccagg tcctcctcga caagtcgctg gaccccgcga agagcgaggt
     7201 gtaccgccgc ttcgcgacgc tcatggagtg ggcggacaag gcgcggcgcc tgaaggtgcg
     7261 ggcgaacgcc gaccagccgg accaggccct gaacgccgtc gccttcggag ccgagggcat
     7321 cggcctctgc cgcacggagc acatgttctt cggcggcgag cgcatcacgg ccgtgcgcga
     7381 gatgatcctc gcggacaaga ccgaggaccg cgagaaggcg ctcgacaaga tcctcccgat
     7441 gcagcgcgag gacttcatcg gcctgttccg ggcgatgggc ccgcggccgg tcacgatccg
     7501 cacgctcgac ccgccgctcc acgagttcct cccgcacggc gagaaggagc gcgagtccgt
     7561 cgcgcgccag acgggcgtcg acccgcgcct catcgcgcgc aaggtcgccg acctctccga
     7621 gatgaacccg atgctcggcc accggggctg ccgcctcggg atcacgttcc ccgagatcac
     7681 gaagatgcag acgcgtgcca tcctcgaggc cgcctgccgc gtgaagaagg aggggatcga
     7741 ggtgaagccg gagatcatga tcccgctcgt ctcgcacgtg cacgagctcg cggaccaggc
     7801 gaaggtcgtg cgcgacgttg cgaagaaggt cttcgaggcc gagggcgtca ccgtggactt
     7861 cctcgtcggg acgatgatcg agctgccgcg cgccgccgtg acggccgacg agatcgcccg
     7921 cgaggccgag ttcttcagct tcgggacgaa cgacctcacg cagacgacgt tcggtctgtc
     7981 gcgcgacgac gccggaaagt tcctgccgga ctacgtgcgg cgcgagatcc tcccgtccga
     8041 cccgttcgag aggctggacc agggcggcgt cggcaagctc gtgaagatgg ggaccgagct
     8101 gggccgcggg acgcggcccg gcctcaaggt cgggatctgc ggcgagcacg gcggcgaccc
     8161 gtcgagcgtc gagttcttcc accggacggg cctcgactac gtgtcgtgct ccccgttccg
     8221 catccccatc gcgcgcctcg cggcggcgca ggcggcgctg aggaagtagt cagccgccga
     8281 gacgctcgag gtccctcttc gctccctcga gcgtcgggtc gaggcggagg gcttccttgt
     8341 aggaggccct cgcgtcgtcc ttcttgccgc cgcgctcgag gacgcggccg aggcgccagt
     8401 gcgccgagcc gttctccggc gccagggaga cggcgcgccg gtactcggcc tcggcctcgg
     8461 cgggcttctt ctccttgagg aggatgaagc cgctcatctg ggcgccgcgc acggggtcga
     8521 tcccgtcgag cgccttctgc tgggcgcgcg ccttgtccat gccgccgccc gcgatgccgg
     8581 gcgcgttcgc gtagtactga aggaggtcca cgcgggcgtc gacgttcttc gggtcgagcg
     8641 cgaccgcctt ctcgaacgag gccttgcact tcttcgccca gcccatctgc gagaagagcg
     8701 acgacttgag cgccttctgg ccgtaggccc ggcccagcca caggtgcgcc tcggcgctct
     8761 cgggcgcctc ccggacgagc ttctcgccgg cctcgacggc cgcgtcccac ttctcggcct
     8821 tgagggcggc cttgacgggc tcgatccgga ggtcatccgc cgcgggggcc gggagcgtga
     8881 gaacgagcgc gagtgcggca aaggcgatct tcatcggggg ttgtacgccg cgggcgcatc
     8941 gaggtttcgg cgcgtgggaa gatcgcgtca tgaacgaccg gccgcgtcgc caccgttccg
     9001 acctccacga gatcgcccgc cgtgccatgc gggagaaggg gctcaccccc gattttccga
     9061 agtccgccct cgagcagctc ggggcgatca cggaacctgc ccccgcgaga gcggacgggc
     9121 tgcgagacct caggtccctc ctctgggcct cgatcgacaa cgacgacagc cgcgacctcg
     9181 accagctgtc cgttgccgag gcgctcccga acggcgagat cagggtcctg gtcgcgatcg
     9241 cggacgtcga cgcgctcgtg aagaagggga ccccgctcga cgagcgcgcg gcccacaaca
     9301 cgacctcggt ctacacggcc ggtggcgtct tcccgatgct cccggagaag ctctcgacgg
     9361 acctcacgtc cctgaacccg gacgtggacc ggctggccgt cgtcatcgag tacgtcgtgg
     9421 acggcggcgg cgcgccgcac ggcacggacg tctaccgggc ggtcgtccgc aacaaggcga
     9481 aactcgcgta cgattccgtc gacgcctggc gcgacggccg gggcccgatg cccgaggcga
     9541 tggcgaaggt ccccggcgtc gacgcgcaga tccgcctgca ggagacggcc gcgcagaagc
     9601 tgcgcgcgcg ccgctacgag gagggcgcgc tcgacctcga gacgatcgag gcgcgtgcgg
     9661 tcttcgacgg cgagacgatc acggacctca gggtcgacca gcagaacgag ccgcgacagc
     9721 tcatcgagga cttcatgatc gcggcgaacg gcgtgacggc gcggttcctc gaggacaagg
     9781 gttacccgtc gctgcggcgc gtcgtgcgct cgcccgagcg ctggctccgg atcgtgacgg
     9841 tcgcctccga gcacgggttc agccttccgc cggagcccga cgcgaaggcc ctcgagacgt
     9901 ttctcaagga gcagcggcgg cgggatccga tgcgcttccc ggacctctcg ctcgtgatcg
     9961 tgaagctcat gggcgcgggg gagtacgtcc tggaggttcc ggggcggacg cccatcgggc
    10021 acttcggcct cgcggtgcgg gactacacgc actcgacggc gccgaaccgg cggtaccccg
    10081 acgtcatcac gcagcggatc ctgaaggccg cgctcgcggg aaggccgagc ccctacggca
    10141 acggcgaact cgcgagcctc gcccggcact gcacggacca ggaggacgcc gcgaacaagg
    10201 tcgagcgcct cgtccggaag gccgcggcgg cgctcctgct tcagaaccgg gtcggcgagc
    10261 gcttcaacgg catcgtgacg ggcgcgtcgg acaagggcac ctgggtgcgg atcttcgcgc
    10321 cgccggtcga gggcaaagtc gtgcgcggcg acgaggggct cgaagtcgga gagcgggtgc
    10381 gggtgaagct gctctcgacc gacgtcgagc acggcttcat cgacttcgcg cgggcgtgat
    10441 cccggccagg aaccggatca ggaactcgtt cacccttgcg ggcgcgtcgg cctgcaccca
    10501 gtggctcgcg tcggggatgc gctcgatcgt gaggttcggc acccagcgat cgaggccctc
    10561 gagaagcccg gggccgaggt aacggtcgcg ctcgccccag atgaggagcg tcttcaggcc
    10621 ttgcggccac gtgcggccgc gcgcccgcgg cggatagcgc agcgccgccc ggtagtagtt
    10681 cacggcggac gtgagcgcgc cgggctgcgc aagggcttcc gtgtagaggc ggacgtcctc
    10741 gtccgggaag gcgcccggcg tcacggggtc gcgccgcagc atcttcccga ggatcgcgaa
    10801 gtcgccggcg cggatccgcc gctcgggaag ccagggcagc tggaagagaa agacgtagga
    10861 ggacttcttc cgctgagcgg gactcgacct cagctcgcgc cggaacgcga gggggtgcgg
    10921 ggcgttgagg agcgtgaggg agaggagccg ctccggaatg aagagcggcg cgtaccaggc
    10981 gaacgcgccg ccccagtcgt ggccgacgac gtgcgcgcgc gactcgccct gttcgccggc
    11041 gagaagcgcg acgaggccct cgagatcgcc gagcagcgct tcgatccggt aggcgcgcac
    11101 gccgggcggc ttctcggaca ggttgtaccc gcgcaggtcc ggcgcgacgg cgcggaagcc
    11161 cgcggccgcg agcgccggga tctggtgccg ccacgcgtac cagaactcgg gaaagccgtg
    11221 gagggggacg acgagcggcc cggcgccctg ctcgacccag tggagccgga tcccgtttac
    11281 gcggccctcg cggtgcgtcc aggcttccgt catgcggccg ccctccccgg ctctttcacg
    11341 cgcgtgaaga gcgcggccga gaggagcccg agcgcgagca tcgtgagcca gagcgtacgc
    11401 ccgccgccgt gctcgaggag cgtcgtgccg agcggcccgc ccaggacgag cgcgaaactg
    11461 aacgccatcg tgtacaggcc catgtaggca ccgcgcttgt ccggcggcgc gacgtccgcg
    11521 acccaggagg acatcgacgg gacgaggatc atctcgccga aggtccagac gacgacggtc
    11581 gcgacgaccg cgggcagcga ccgggcccac gcgagcgccc cgaacccggc cgcgacgagg
    11641 acggagccca ggacgaggga tcggcggtgc ggccagtggg ccatcgcgag gttgagaggg
    11701 acctcgacga agacgatcag gagcgtgttc acggtgtgca ggaggccgaa gatccagacg
    11761 gcgaggccga cgtcgcgcac gaggaagacg gacatcgagg actcgtgctg gaagaagacg
    11821 atgcagaccg gcacgagagc cacgaggaag aacacgaggg ccgggtcgcg gaggccttcg
    11881 cggcgggacg cagcggcgcc gctcgagggc tcggcggccg gcagcgcgtt gcgcggaaaa
    11941 aagaagatca gggtggccgc ggcgagcaga gaagtcacgc cgtccacgat gaagagcgcg
    12001 ggccacgaca ccgccgcgag gaagccgccc gccgcgggcc cgacgctcat cccgaggttg
    12061 acggcgaggc gttggagggc gaacgccgcc ttgcgctggt cgggcgccgc gaggtgggag
    12121 acgacgctca ggctggcggg gcggaacatc tcgcccgtga gcgcgatgag aagcgtgagc
    12181 acggtgatcg ccggcatccc gcgcgcgaaa gggacgagga gaaggagggc gcccgaggcc
    12241 atgagcgcga tgcgcatcag gcgcacggcc ccgatccggt ccgcgagccg tcccgcgacg
    12301 ggtgcgatcg ccagcgatcc cgcgccgaag acggcgaaga cgaggcccgc ggcgccgggc
    12361 gtgaagcctc gctcgctcgt caggtagagg acaaggaacg gcaggaacat cgtccccgac
    12421 ctgttcacga gagaggcgcc cgccagcacc cacagggcgc gagggaggcc ggagaggccc
    12481 cgccacgggt tcaagggggc cccccgggaa gcggcgtcac gggaagagca tacgtgcgcc
    12541 gggagggacc gtccgcccgg ccccggcgtg gataatgggc ctgtgcacgt cgagctgacg
    12601 ctcaacggcg ccgcccgggg cttcgacgtc gcggccgacg cttcactcct cgagctgctc
    12661 cgcgaccacg cgggcgtcac gtcgccgaag gacggctgct cgccgcaggg ccagtgcggc
    12721 gcgtgcctcg tcctcgtgga gggccacccg aagacggcgt gcgccgtgcc ggcgcggacg
    12781 gccgcgggga agagcgtcct gacgctggag ggcgtgcccg aggccgagcg ccacgtctac
    12841 ggcgaggcct tcgcgcggac ggcgggcgtc cagtgcggct tctgcattcc gggaatcgcc
    12901 ctgaggatga agagcgttct cgacgcaaac ccggacccga cggacgagga gatccggaag
    12961 aagctcgacc cgcacctctg ccggtgcacg ggctacgtca agatcgtgga cgcgttccac
    13021 gcggcggcgc gcctcaggcg cggcgaagcg ctcgagccgg cggacgactc gggccgcgtc
    13081 ggcacgagcc tcgaccggta cgaggccgag gagctcgcgc tcggcgcccg gccgtacgtc
    13141 gcggacatgg cgtttcccgg gatgctgcac ggcgccctcg cgctctcgcc ccacgcgcgc
    13201 gcccgcgtcg tcgccgtcga cacgtcgcgt gcggcgcgcc acccgggagt gatggcgatc
    13261 gtgacggcga aggacgtccc cgggcagcgc ttctacggcc tgatccaccc ggactggccc
    13321 ggcttcgtgg ccgagggcga ggagacgcac tacgtcggcg acgtcctggc ggcggtggcg
    13381 gccgtggacg cgcgcacggc ccgtgcggcg gcggcgctcg tcgacgtcac gtacgaggtc
    13441 ctgccccccg tgaccgaccc cgacgaggcc ttgcgtgacg gcgcgccgcg cgtgggtccc
    13501 gagggcaacc tcctgtcgcg ctcggtcgtg acgctcggcg acgcggacgc ggcgctcgcc
    13561 gcctcggcgc acgtcgtcga gggcacgtgg cagacccagc gcatcgagca cctgtacatc
    13621 gagcccgagg cctgcctcgc cgcgccggga gacaccgcgg gccagaaccc gaaggcgcgc
    13681 ctgaccctgt acacgcaggg gcagggcatc ttcgacgacc ggcgccaggt cgcgggcttc
    13741 ctcggactgc ccgaggagga ggtccacgtc gtcctcgtcc cgaacggagg cgcgttcggc
    13801 ggcaaggagg acatgtccgt ccaggcgcag acggcgctcc tctcgatcgt aacggggcgg
    13861 cccgtgcgtc tcgtcctctc gcgcgaggag tccatgcgtc tccaccccaa acgccatccg
    13921 atccggatgc actaccgggt cgggtgcgac gcggagggcc ggctcacggc cgtccgcgcc
    13981 cggatgctcg gcgactcggg cgcgtacgcg tcggtcgggg gcaaggtact cgagcgcgcg
    14041 gccggccacg cggccgggcc gtaccggtgc ccgaatctcg acgtcgaggc gctggccgtc
    14101 tacacgaaca acccgccctg cggcgcgatg cgcggcttcg gcgcgaacca ggcccacttc
    14161 gcgatggagg gtgccctcga catgctggcc gaaaaggccg gcgtcgaccg ctgggagatc
    14221 cggtggcgga acgcgctcga ggcgggcgac gactggtctt gcggccagac gctcaccgcg
    14281 tccgtcggcc tcaagaagac gctcctcgcc gtgaagaagg tctgggacgc gagcggaggc
    14341 cgcgcgggca tcgcgtgcgg catcaagaac acggggatcg ggaacggcat gaaggagtgg
    14401 gggcgggcgc ggctcgtcgt cggctccgac ggttccgtga gcatccacaa cggctatacg
    14461 gagatgggcc agggcctcct tacggtgctc atccagtgcg cgtgcgagac gacgggcctt
    14521 cccccttcgg tcttccgtgc gaaggtcgac acgcgcttcc cgatggaagt cggccagacg
    14581 accggctcgc gggcgacgta cctcggcggg cgcgccgtcg tcgacgcggc gaagaagctg
    14641 aaggccgacc tcgattccgg gaaaacgctg gcggatctcg ccgggaccgt ctacgtcggc
    14701 gagacgctcg tggacgacac gacgaagctc gggcacgagc cgccgcccgg gaaacccatg
    14761 aagctccact cggcgttcag cttcgcgacg caggtcgtcg tgctggacga gaagggccgc
    14821 gtcgcgaagg tcgtcgccgc ccacgacgtg gggcgcgcca tcaacccgaa gctctgcgag
    14881 gggcagatcg agggctcggt ccacatgggc ctcggctacg cgctcacgga ggagatggtc
    14941 tgcaaggacg gcatgccgac gtcgttccgc atccgcgacg tgggcgtcct gcgggcgcag
    15001 gacatgccgg aggtcgacgt gatcctcgtc gaggatcccc agcccgacgg cccgttcggc
    15061 gcgaagggag tgggcgagat cggcctcgtc cccaccgcgc ccgcggtcgc gggggcgctc
    15121 ttcgccttcg acggcgtccg ccggttcaca ctgcccatga aggactccgc ggccgcgcgg
    15181 gcgctgaaga ggtgacggcg tgagtcctct gctgccgaag gtgaccggtc tcaggtgcgt
    15241 cctctgcgcg gccgtctacg cgcacggcga ggtcgagtac acgtgcccgc gctgcgggat
    15301 cgaggggatc ctcgacgtcg agtacgacca cgacctcgtc gcgcggacgt tcggcaggga
    15361 ggcgctcgcg gccaaccgcg atctctcgct ctggcgttac cgcgccctcc ttcccgtgcc
    15421 ggacgacgcg gccgcgcttc cgcctccgcg cctgcaggtc ggcttcaccc cggtcgtcga
    15481 gtcgccggtc ctcgcgaagg agctcggact cggcgccttc ttcgtcaagg acgacgggcg
    15541 aaaccccacg gcttccttca aggaccgcgc gagctcggtg ggcgtcctgc gcgcacggtg
    15601 gctcggccgg aacgagatca cgtgctcgtc gaccggcaac gcggcctcgt ccctcgcggg
    15661 cttcgcggcc gaggcgggaa tgaacgcgtt catcttcgtt ccggccaccg cgcccgaggc
    15721 caaggtcgcc cagctcctcg tctacggggc gaaggtcttc ctcgtcgagg ggccgtacga
    15781 ggacgcgttc cgcgtctgcc aggccgcggt cgaggagttc ggctggtaca accgcaactg
    15841 cgcgatcaac ccgtacctcg tcgaggggaa gaagacgtgc ggcctcgaga tcgccgagca
    15901 gatgcgcgag aggctgcccg acgtcgtgac ggtcgcgctc ggggacggct gcacgacggc
    15961 ggggatctgg aagggcctcc tcgagatgaa gacccacggc gtcgtcgacc gcctgccgcg
    16021 gatcctcgcc gtccaggccg agggcgccgc gccgctcgcg atgaccttcg cccgcggcga
    16081 cgagcggatc gaccccgtgg acgcggcgac gctcgccgat tccatcaacg tcggcgcgcc
    16141 gcgcaacggc atcaaggccc tgcgcgccgt gcgtgcctcg ggcggcgcca tcgtgaccgc
    16201 gcgcgacgag gcgatcctcg cgtggatccc gcgcctcgcc cgcgcgacgg gcgtcttcgc
    16261 cgagcccacg ggagtcgccg cgctcgccgg cctcgaggcg gcgctcgaga agggcctcgt
    16321 ccgccgcggg gagcgcgtcc tccacgtcgc gaccggcaac ggcctgaaag acacgcgggg
    16381 agccatgcgc tcggtcggtc ccccgacacg cattccgccg gacctcgacg cggtccggcg
    16441 cgcactggag aagaaatgat tcacggcccg acctacgccg aaatgcgcga acccggcctg
    16501 ctgcccgcgg aactccggaa acgcgcgcag acggccgcgc gcgaagcgcc gctcgatccc
    16561 gtcaacctgt tcaacatcac ctggcacgac gagggcggcc gcgtccgcgc gctgcggctg
    16621 cccgaggcgc tcacgggcgt cccggcgccg atcgtcctcc tctacgcgaa ggacttcccg
    16681 acgggcgcgc acaaggtggg ggccacgtac tcggtcctca tggagcacca gctcgcgggc
    16741 gacgtcacgc ccgggacgtc gacgctcgtc tggccctcga cgggcaacta cggcatcggc
    16801 ggctcgtggg tagggccgcg catgggctac gagtcggtcg tcatcctccc ggaggagatg
    16861 agcgcggagc gcttcgagaa gatcgccgcc tacggcgcgc gcgcgatcaa gacgccgggc
    16921 tcggagagca acgtcaagga gatctacgac gagtgcaagc ggctccgggc ggacccgaag
    16981 atccgcgtga tgaaccagtt cgaggagatg gcgaactacc gctggcatta caacgtcacg
    17041 ggcgcggccg ccgcagaggc cgtcgaggcg ctgggcgcgg gccggcccgc ggcgttcgta
    17101 tcggccatgg gctcttcggg cacgatcgcg gcaggcgacc tcctgaaggc gcgcttcggg
    17161 acgcgcatcg taggcctcga gccgatccag tgcccgacga tctcgcggaa cggctacggc
    17221 gcgcacgcga tcgaggggat cggcgacaag cacgtgacgt ggatccacaa cgtcatgaac
    17281 atggacgccg tgatggcgat cgacgaccag gagtgcctcc tcggcctgca gctggtcgcc
    17341 gagcccgagg gacgcgattt cctcgccgag accggcgggc tcggggaagc cgcgcaccgg
    17401 ctctccacgc ttctcggcgt cagcggcgtc tgcaacatcc tcggggcgat caagaccgcg
    17461 aagtggctcg gcctcgggaa ggacgacgcc gtcgtcacgg tcgcgacgga cggcctcgac
    17521 cggtacccgt cggtcctgcg ccgcctcgag aagacgacgg gcgcactcac gcgcgagaag
    17581 gccgaggctc atctcgagtc catcttccac gggcagaagc tggactggat ctcggaaggg
    17641 acgagcgaga gccgcgagag gtggcacaac ctgaagtact tcacgtgggt ggagcagcag
    17701 gggaagaccg tcgcggagct cgatgcccag cggtccggga actggtgggc ccacgaggcc
    17761 ggactcgccg gcgacgtcga ccgccggctc cgcgaggtgc gcggctggtg acggagtctg
    17821 ccgccggcga aaccggcgcg ggcgccctcg cgatcacggg gggcaccgtc gtcacgctcg
    17881 accgcccatc ggtcgagcgg cgcgacgtgg tcctcgggac cggggcgcgg cggacgctcg
    17941 acgcgtcggg ctgtctcgtc ctgcccggtc tcgcgatcgc ccacacgcac ctctactccg
    18001 cgctcgcgtg cgggatgccg gggcccggcg agccgccgcg ctccttccgg gagatcctcg
    18061 agaaggtctg gtggcggctc gacgccgcgc tcgacgagaa atcgctcgag gcctcggcgg
    18121 acctcgcctt gctcgacgcg gtgctttcgg gcgtcacggc ggtgatcgac caccacgagt
    18181 ccccgtcgtt catcggcgac tccctcgacg tcctcgcgcg ggcctcgcgg tcctccggcg
    18241 tcaaggccgc cctctgctac ggcgcgacgg atcgccacgg cgccgacggg gcgcgcgcgg
    18301 gcctcgccga gtgcgagcgt gcgatccggg ccggacttcc cgcgatggtg ggcctccacg
    18361 ccggcttcac ggtctcggac gcgacgctcg cctccgccgc ggacctcgcg aaacggacgg
    18421 gacgctggct gcacgtgcac gccgcggagg accgctgcga cgcgggctcg ttcgagcgcc
    18481 tcgagaaggc cggccgccac ttcgccgcgc gccagatcgg ccgact
//
LOCUS       DAOMFY010000049        18344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_950_length_18344_cov_8.766964, whole genome shotgun sequence.
ACCESSION   DAOMFY010000049 DAOMFY010000000
VERSION     DAOMFY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18344
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_950_length_18344_cov_8.766964"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLB01_06175"
     CDS             complement(<1..599)
                     /locus_tag="PLB01_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQQ76922.1"
                     /translation="MSRPSSRELAPGPFLVCGMGHVGYRIVELLTRLGEPVVVVTRET
                     RSEWQRAAAAAGAKVVMGDARDPELLRAAGLMEARGLLAVTDHDPVNLEIALDARRLR
                     PDLPVVARLFDQNLAQQLEATHGVRRALSVSALAAPAFAGAALGESILASFASQGKLD
                     VVVRGEDGALLVLEENEARARLESRRDAPRPGLPRPAASG"
     gene            782..1471
                     /locus_tag="PLB01_06180"
     CDS             782..1471
                     /locus_tag="PLB01_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76923.1"
                     /translation="MKKLGMTVLAAALAATLAPAAAASDRPAPPAASASRVGAPDLLF
                     QEVQVHYPGLNLSIRLVAPGGPSTTSVLRLVENPAVAPGTKLGPDEPNGALGPVATKS
                     CEARLVSNLRVWVKNQGNKGFDATTAALGLTGNVDAAAVTKAFGKVDAGATNYFEAGS
                     PALLPGWHSAHLVLNSAKGQGESNFSNNVFDGRFEITCEKKKPAPPCPQGQVRDPLTG
                     QCKPAPSAAPK"
     gene            complement(1478..1918)
                     /locus_tag="PLB01_06185"
     CDS             complement(1478..1918)
                     /locus_tag="PLB01_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQQ76924.1"
                     /translation="MYVQDVMKKKVVTCREKDSIKKCADLMREWKIGMLPVVDAKHQL
                     VGIVTDRDLVVRGIAESRPLTTEAGDIMTKRLVTCRDGDELWFAEERMAAERKSRIVV
                     VDDSRHVVGVISLSDIPMAEEGARAGELLRQVARREAAPRPERH"
     gene            2121..2426
                     /locus_tag="PLB01_06190"
     CDS             2121..2426
                     /locus_tag="PLB01_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76925.1"
                     /translation="MAIIPVARVKVERAGRVLLDGSPVTLAALRKALPGLKKKKARIR
                     YYREGSRTEATKQALAVFELVMDSGLPVELCDDEAQFQGDRLGLPKEEKPDDDSWWG"
     gene            2525..4090
                     /gene="aceB"
                     /locus_tag="PLB01_06195"
     CDS             2525..4090
                     /gene="aceB"
                     /locus_tag="PLB01_06195"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HQQ76926.1"
                     /translation="MDGIQVLSRAPEATDTILTKEALDFVARLHRTFDPRRQELLARR
                     AERQKRFDAGELPVFLPETQAVRNADWKVASAPPDLQKRWVEITGPVERKMMINALNS
                     GASVFMADFEDSLSPTWANVVQGQQNLKDAVRRTIAYTSPEGKEYRLAPEIATLLVRP
                     RGWHLPEKHVLVDGAPISASLFDFGLYFFHNAYALKERGTGPYFYLPKMESHLEARLW
                     NDVFVAAQEGLSIPRGTIRATVLVETLPAAFEMDEILFELKDHMSGLNAGRWDYLFSL
                     IKKLRAHADVLPDRGQVTMTVPFMRAYAELLVKTCHRRGAHAMGGMAPFIPTRKDPEV
                     NERALAKVRADKDREVADGFDGTWVAHPDLVPTAWEVFEKAFGTKPNQKDRLREDVVA
                     DARKLTDLSVPGGTVTDAGFRLNVNVTLQYLNSWLLGNGAAAIYNLMEDAATAEISRA
                     QLWQWINRNATLADGRTATKDLYRQVRDEEIPRVGGIANGRMREAVEILDRLVLDDTF
                     AEFLTLPAYAYLE"
     gene            complement(4137..5552)
                     /locus_tag="PLB01_06200"
     CDS             complement(4137..5552)
                     /locus_tag="PLB01_06200"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQQ76927.1"
                     /translation="MTNRREFLKLACGAAGFAAFEQGLDRFGLLAHAASAAPPNQYRA
                     LVCIFMFGGNDANNMVIPVTAGGLSGYNQYATSRGASLALPPLGATAFTSPTSFGLNF
                     GLHPAMPELAALYNAGKLAVVSNVGPMTRWPTTKALYQSNAAYRPYQLFSHSDQQSAW
                     MAPQSDVQSIYGWGGRVVDMISDLNTGTSFPASLSLSGNNQFNVGQTGKALNVAPAPT
                     ALNAIFPLNGFANNAIDNARRTAFNTLRTFDTGVPLVKAASDITQQGLDIQASLATDP
                     VVTATFPATGLGNQLKQVAKIIKQNQVSLNVSRQVFFCSIGGFDTHQDELASHTNLYT
                     QISKAMAAFYQEMFNQNLHNNVTTFTMSDFGRTMAPSGSGASVGSDHAWGTHQLVMGG
                     AIKAADFYGNAGLNGTPFPDLTLGSQYDTDSRGRWIPSTAVDQYAATLGLWLGATPAE
                     LNTVFPNLYRFPTADLGFLNP"
     gene            complement(5555..7561)
                     /locus_tag="PLB01_06205"
     CDS             complement(5555..7561)
                     /locus_tag="PLB01_06205"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HQQ76928.1"
                     /translation="MKYCLSARSVALLSLLLPGAVLAQSPQSKFYPITPCRAVDTRNP
                     NGASGGPALAAGGARAFQLAGSCGVPVTARAVVTNLTVVNPSSAGDLAIFPTGTPSPT
                     GATAINFKAGRTRANNFIARLGTTGDIAVFTEMPSGTVHFLIDVSGYFEDPPPSPLST
                     YTAVANLTSFGATPQLVAHIRDVGVNGWLNEQFSIPPNYFVPAALFPDTIPGACTGTC
                     QRDNYTMYPLQSTFLYNALYYPDQLRMRVFWALHKFLVVSGQQEIQPSHMIPYLNILY
                     GNAFGHYRDILYQLTVNPAMGDYLNMRTSTLQNPNENYAREILQLFSIGLVQLNTDGT
                     PKLDLGGNTIPTYNQNDINELSRVFTGWRLAAPPVAGTSNYTSDMVLVEASHDRGKKS
                     FLCDWSTGTPTACAWTFNANQNGDVDVAQAIDAIMAHPNVAPYVSTQLIHGLVTSNPS
                     PAYVGRVAAVFNNNGSGVKGDLAAVVRAIVTDSEALAGAADPNFGILRDPVYHVIALL
                     RGLGATAANGSGRSDGVLAPQLANLGQNLFNPDTVFGYYPADNRLPGSSTMWGPEFGI
                     QSALTALRRANLVNTLVYSTIPTGANNPSGTALDLSVIQSLSNDPAAMVEQLNQLLCR
                     GQLSAGAKSAIVTAVNAVPSTSPKQRAQQAVYLVATSSQFQVER"
     gene            complement(7739..8149)
                     /locus_tag="PLB01_06210"
     CDS             complement(7739..8149)
                     /locus_tag="PLB01_06210"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HQQ76929.1"
                     /translation="MAPDRDLRLDVPAIDAEHALQVELVEALGRAVAEGKSREVADEI
                     LDKLLDYTRVHFLAEELMMRMEGYPGYEAHRGEHGELLAELQKLRAAYESGALPMTHV
                     AVDGLRSWLSGHVRTQDRGFANFLASKAAARSSV"
     gene            complement(8162..9103)
                     /locus_tag="PLB01_06215"
     CDS             complement(8162..9103)
                     /locus_tag="PLB01_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HQQ76930.1"
                     /translation="MTFWSGRRVLVTGGGGFLGQALVEALRRRGAREVLAPRAADLDL
                     RERDAVRAFLTREKPDVVVHAAAVVGGIGANRAHPGRFFYENAIMGIQLIEEARRAGV
                     AKFVCLGTICAYPKFTPVPFREEDLWNGYPEETNAPYGLAKKMLLVQLQAYRQEYGFD
                     GIYLLPVNLYGPRDNFDLATSHVIPAMIRKFVEAQAAGAPEVVLWGDGSPTREFLYVD
                     DAAEGIVAAAEAYDGAKPVNLGSGEEIAIRDLAQRVAGAIGFAGSIRWDASRPNGQPR
                     RRLDVSRAAGLFGWRARTTLSEGLGKTVDWYRATLRP"
     gene            9252..10652
                     /locus_tag="PLB01_06220"
     CDS             9252..10652
                     /locus_tag="PLB01_06220"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQQ76931.1"
                     /translation="MKTALLLRSLTFVVLLSATMSPADVVIPSSVYRRGAGGAEFRSD
                     VRVFNPTTSPVNYTPVLYDQVTGQTFTAAIQTIPSRTQQSFDNIVGTLFGRTLDNGSF
                     GPIRFQTSGTLLVSSSVNNYNACGNGSVSGQWLPGLDAAAALKAGTLVQLASSVDLAA
                     GYRTNVVFTNPGSSDATVRAKVRRGDGTLVSDATLPALGPNGFTQIGNWGPPFSGVAG
                     TNDTNLWIEFTSDQPVLSFASVINNASGDPFAIVMTSEPAAPPAAPVANYTVSASPQP
                     NQPVTFTDLSTGTPTNRFWVFGDGTFATNPVAPVQHTYTAAGTYKSALFVDNAAGASS
                     KTQDVVVASATPIAVTISATTTNNTKWTFVCQTGPCSGAGNNNVNLKVGQPYTITWTT
                     PASEAKTHGVGGIAVLNITQCDVIRSNQPCTASLTPTASMLNFPGPVYTYFCTQSACG
                     TQPQHDGMSGTITIVP"
     gene            complement(10710..11651)
                     /gene="ligD"
                     /locus_tag="PLB01_06225"
     CDS             complement(10710..11651)
                     /gene="ligD"
                     /locus_tag="PLB01_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HQQ76932.1"
                     /translation="MRPLSPLAPMLATRVKEPFSRRGWVFEPKYDGWRIVAIRRKGRV
                     TLATRRGFDIAEELPDIVKAVEALPGGNLAVDGELVVLDDRGVSSFQLLQARNEPGAR
                     PPVLVLFDCLDADGVSLMSLPLGERRAALEALVGKHPAPPLALAERLGTDGAAAFAKA
                     VKKGWEGVVGKDLSSPYESGRRSLRWLKVKVRREQEFVIGGLTLPSDPRPHFGALLVG
                     LYEGKHLRFCGAVGTGFSDKILLSLLEKLEPLHQDACPFSPPPHGVKDAAWVKPQVVA
                     QVAFAEWTGELRLRQPSFLGLREDKDPRECLWAERET"
     gene            complement(11662..13086)
                     /locus_tag="PLB01_06230"
     CDS             complement(11662..13086)
                     /locus_tag="PLB01_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQQ76933.1"
                     /translation="MKKTLLPSLPVPGVTPVTGADLSRRGFLRLGGSGLVASWFLGSP
                     AAAWAARSAKVSTRGTARNAIFVFLPGAPSQTDTWDLKEGAWTPSDFAPTSFGGGVRF
                     PAGLMPNLANRIGDLAFVRSLKSAALVHGLNQTWLQIARNPTGATGAIAPNIGSVVAL
                     ELEKQRGPNDVLPGFFAFNTGTAGLADEGYLPSTVAPFVAQPIATGLPNLTHPDGAAA
                     LATRYAWMQELDAALRTGQPMGKEPADAPDLYRQAKLLVDSPDVNQFLTIAAADSARY
                     GSSAFGDSCLLAKQVLAAGRGTRFVMLGLGGWDHHASIYKKSVNANLGETSLYSLAVQ
                     LDAGLASLIGDLKATPGKTAGKTLFDETLLFVVGEFGRTVGPLGDAAGRDHFANYCAV
                     FAGGGVKGGQVIGATDATGATITDSGVTGRTELRAEDLACTVYSALGIDWTTTRRDDP
                     IGRGFEYVPNAGAGVYAPIDRLFG"
     gene            complement(13086..15053)
                     /locus_tag="PLB01_06235"
     CDS             complement(13086..15053)
                     /locus_tag="PLB01_06235"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HQQ76934.1"
                     /translation="MRNRTGCCGAARRLALRVRDLLFLAVAIVPPGRPAEAVETGGAE
                     AMVADGTVRPATSDCAFVPSHADPDGRRRREAVSRDTRAFASAHPAASSTAFHPASRL
                     KRVNYVDDEIFGKMEADRVPPAPRSSDAEFLRRVTLDLTGRIPDADTVIAFLADPSSD
                     KRSRMVDMLLTTDAFVDRWTFFYDELVQNTNRSSTGTLWDTANAAYHAYFQDAVRSRK
                     PWDVMARELITASGPNTTVGPANFIVRQFQNNGPPQDTYDNLAVATSRVFLGTGVVFC
                     TSCHNGQGHLDAINLWGSIVKRQDFWSLAAFFSWTDMPFAGPVPNTVYTIGQRRTGNY
                     DYRLNTTTGNKTDRTGAYYTTTPPGLTTISPAYPRTPLNPSPGGILPGETFRQWLARL
                     VTDDPQFARAAVNTLWKEMFKLGIVEPVDGFDPMRQDPASPPPGAWTVQPTHPALLNR
                     LAQDFAAHGYDLRYILGVMAKSNAYQLSSFYPGTWSEAYTPYFARHFARRLGAEELWD
                     AVQKATNVPSNMTVSGSPALALQLPNMGFQNGPDLNGRFMSAFGPGNRDTVPRSWKFS
                     VAQALTMLNNNQVTIRSHDTQDSVVHRLVAANATPEQAVDTLFLKTLSRVPTPAEKAW
                     CLSKAQGTSLPSFAQTLQFVLLQKLDFLYNY"
     gene            complement(15111..16511)
                     /locus_tag="PLB01_06240"
     CDS             complement(15111..16511)
                     /locus_tag="PLB01_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQQ76935.1"
                     /translation="MKPLLPSFPASGATPVNGPDLSRRGFLQLGAGGLVASWFLKSPA
                     TAWAAESAAVTPRNTAKNVIFVFLPGAPSQVDTWDLKEGAWTPADFAPTGFGGGLRFP
                     AGLMPNLANRLGDLSIVRSMKSAALVHGLGQTWLQIARNPTGATGAEAPNMGAVAALE
                     LEKQRGPNDVLPAFLSLNTQAGLSGAGYFPSTYAPFVVQPTANGLASLTHPDGATRLG
                     TRWNDLAQLDAALRSGQPLGKDAADAVNFYNQAKVMIDTPAVNDLFKYGAADAQRYGN
                     SSFGNACLVAKQLLAGGRGARFVMVSVGGWDMHSNIYAKTGTSLYSQMGQVDPALASL
                     IGDLKATPGKTPGKTLFDETLIVMAGEFGRTVGNLNGQSGRDHFAVYSAVFAGGGVRG
                     GQVIGATDATGATITDSGVTGRTELRAEDLACTVYSALGIDWTTMRHDDPLGRGFEYV
                     PNAAAGVYAPIDRLFG"
     gene            complement(16520..18187)
                     /locus_tag="PLB01_06245"
     CDS             complement(16520..18187)
                     /locus_tag="PLB01_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HQQ76936.1"
                     /translation="MDDEIFGKMDAARVVPAPLASDAEFLRRVTLDLTGRIPDAATAA
                     AFFADPAPDKRGRMIDTLLASDAFVDRWTFFYDEMFQNTAFAASGRLYPQGRNAFHAY
                     FQDAVRSHKPWDAMAREIIGATGVNTTVGAANYVVRQIQANGPIQDTYDNLAASTAAV
                     FLGTNAVFCTSCHNGQGHLDAINLWGSTVKRQDFWGLASFYSRATAPRSGTANANYYY
                     TVGERGTGNYLLNTTTGNKTDRTKAYYTTSPAGMTSIDPAYLRTPLAPAGGAPASGEG
                     FRQALGRLVTNDPQFARAAVNYLWKEMFGVGIVEPADAFDPMRQDAASPPPGSWTLQP
                     THPALLTKLAQDYAGHGYDLRYILGLMAKSSAYQLSSFYPGAWSESYAPYFARHFARR
                     LTAEEMFDAITRATNVGASIPVSGGMPNVTLAMQLPDVQEPGAGSVIGNFLNAFLRAD
                     RDGDARSNEFSVSQALLMLNDTTVTSRIKSATAGSAVQKLVAANATPTQIVTSLYLST
                     LSRSPSAAELAAGVALFASPPNGETKAQVAEDLQFALLNKLDFLYNY"
BASE COUNT         2811 a         6229 c         6518 g         2786 t
ORIGIN      
        1 ccggacgcgg cgggccgcgg gagcccgggg cggggcgcgt cccgccgcga ctcgagccgc
       61 gcgcgcgcct cgttctcctc gaggacgagg agggctccgt cctcgccgcg gaccacgacg
      121 tccagctttc cctgcgaggc gaacgaggcg aggatcgatt cgccgagagc ggcgccggcg
      181 aaggcgggcg cggcgagcgc ggacaccgac agcgcgcggc gcaccccgtg cgtcgcctcg
      241 agctgctgcg cgaggttctg gtcgaagagg cgggcgacga ccggcagatc gggcctcagg
      301 cgccgggcgt cgagcgcaat ctcgaggttc acggggtcgt ggtcggtcac ggcgaggagt
      361 ccgcgcgcct ccatgaggcc ggcggccctg aggagttcgg ggtcgcgcgc atcgcccatg
      421 acgaccttcg ctccggccgc cgcggccgcg cgctgccact cggaacgcgt ctcccgcgtc
      481 acgacgacca ccggctcacc gagccgggtc aggagctcga cgatccggta cccgacgtgc
      541 cccatcccgc acacgagaaa gggcccgggc gcaagttcgc gtgaggacgg tctgctcatc
      601 ggaggcagcc gccattatcg cggacaccgg accccgtgac tcgggtcacg acgacgagcc
      661 cgaggcgcgg caggaccacg ccttgacgcc ccggagccat gcgtgggcgt tgtccgggcc
      721 ccggtctccg cttacgttta ccccgacgac gcggccgaga gccgctcgca cggaggacca
      781 cgtgaaaaag ctcggaatga ccgttctcgc cgcggccctg gccgccacgc tcgcccccgc
      841 cgcggccgct tccgacaggc cggctccgcc ggccgcgtcc gcgtcgcggg tcggggcgcc
      901 cgatctcctt ttccaggagg tccaggtcca ctacccgggc ctgaatctct ccatccggct
      961 cgtagcgccg ggcggcccgt ccacgacgtc ggtgctccgg ctcgtcgaga acccggccgt
     1021 cgctcccggc acgaaactcg ggcccgacga gccgaacggc gccctcggac ccgttgcgac
     1081 gaagagctgc gaggcgcggc tcgtctcgaa cctccgcgtc tgggtcaaga atcagggaaa
     1141 caagggcttc gacgcgacga cggcggccct cggactgacg ggcaacgtcg acgcggcggc
     1201 cgtcacgaag gccttcggaa aggtcgacgc gggcgcgacg aactacttcg aggcgggctc
     1261 gcccgccctc ctgcccggct ggcactcggc ccacctcgtc ctgaactcgg cgaagggcca
     1321 gggtgagtcg aacttctcga acaacgtctt cgacggacgc ttcgagatca cgtgcgaaaa
     1381 gaagaagccc gctccgccct gcccgcaggg ccaggtccgc gacccgctga cggggcagtg
     1441 caagcccgcc ccgtcggccg cgccgaagta aacggcgtca gtggcgctcg ggccgcggcg
     1501 ccgcctcgcg gcgcgcgacc tgccggagga gttcaccggc ccgggcgcct tcctcggcca
     1561 tggggatgtc cgacaggctg atgacgccga cgacgtggcg gctgtcgtcc acgacgacga
     1621 tccgcgactt gcgctcggcc gccatccgct cctcggcgaa ccagagttcg tccccgtcac
     1681 ggcaggtgac gagtcgcttg gtcatgatgt cgccggcttc ggtcgtgagg ggccggctct
     1741 ccgcgattcc gcgcacgacg agatcccggt ccgtgacgat gccgacgagc tggtgcttcg
     1801 cgtcgacgac cgggagcatt ccgatcttcc actcccgcat gaggtcggcg catttcttga
     1861 ttgaatcctt ctcccggcag gtcacgacct tcttcttcat cacgtcctgg acgtacatgg
     1921 acgcctcctc gcggccacac cccacacggg atcccgcgat ccgcgcgcgg accgccgctg
     1981 aatgcaacgt gggagcccga aggggcgcgc cgcgagggat tcacgtgcat catgcgccgc
     2041 cggagccccc cggcaggttt gatgcaatgg cacgattctc gagacggccg gaaccgtgga
     2101 gaatccccac ggagacgagc atggcgatca tccccgtcgc gcgggtgaag gtcgagcgtg
     2161 ccggccgcgt ccttctcgac ggcagtccgg tcacgctcgc ggcgctcagg aaggcgctcc
     2221 ccggcctgaa gaagaagaaa gcccgcatcc gctactaccg cgagggctcc cgcaccgagg
     2281 ccacgaaaca ggcgttggcg gtgttcgagc tcgtgatgga cagcggcctc cccgtcgagc
     2341 tgtgcgacga cgaggcgcag ttccaggggg accggctcgg gctcccgaag gaagagaagc
     2401 ccgacgacga ctcctggtgg ggctgaggcg cggcgcgaca cccgccccgg ccctacattt
     2461 ggccccgtga gtctccgggg gcggagcttc gcctccataa tccccggcct cggagggaaa
     2521 actgatggac ggcatccagg tcctgtcgcg cgcgccggag gcgacggaca cgatcctcac
     2581 gaaggaggcg ctcgacttcg tcgcccgcct ccacaggacg ttcgacccgc gccgccagga
     2641 gctcctcgcg cgccgcgccg agcgccagaa gcgcttcgac gccggagaac tgccggtatt
     2701 cctgcccgag acgcaggccg tccgcaacgc cgactggaag gtcgcttccg cgcctcccga
     2761 cctccagaag cgctgggtcg agatcaccgg gcccgtcgag cgcaagatga tgatcaacgc
     2821 gctcaactcc ggggccagcg tcttcatggc cgacttcgaa gactcgctct ccccgacgtg
     2881 ggcgaacgtc gtccagggcc agcagaatct caaggacgcc gtccgccgca cgatcgcgta
     2941 cacgagtccc gagggcaagg agtaccgcct cgccccggaa atcgcgacgc ttctcgtgag
     3001 gccgcgcggc tggcatctcc cggagaagca cgtcctcgtg gacggcgcgc cgatctccgc
     3061 gagcctcttc gacttcggcc tctacttctt ccacaacgcg tacgcgctga aggagcgcgg
     3121 caccggaccg tacttctacc tcccgaagat ggagagccac ctcgaggcgc gcctctggaa
     3181 cgacgtcttc gtcgcggcgc aggagggcct ctcgatcccg agggggacga ttcgcgcgac
     3241 cgtcctcgtc gagacactgc cggccgcgtt cgagatggac gagatcctct tcgagctcaa
     3301 ggaccacatg agcggcctga acgcgggccg ctgggactac ctcttcagcc tgatcaagaa
     3361 gctgcgcgcg cacgcggacg tcctgcccga ccgcggacag gtcacgatga cggttccgtt
     3421 catgcgcgcg tacgccgagc tgctcgtgaa gacgtgccac cgccgcggcg cgcatgcgat
     3481 gggcggcatg gcgccgttca tcccgacgcg caaggatccc gaggtgaacg agcgggcgct
     3541 cgcgaaggtc cgggccgaca aggaccgaga ggtcgccgac ggcttcgacg gcacgtgggt
     3601 cgcgcacccc gatctcgttc ccacggcgtg ggaggtcttc gagaaggcgt tcggcacgaa
     3661 gccgaaccag aaagaccgcc tgcgcgagga cgtcgtcgcc gacgcacgga agctgacgga
     3721 cctctccgtg cccgggggca ccgtcacgga cgccggcttc cgcctgaacg tgaacgtcac
     3781 gctccagtac ctgaactcgt ggctcctcgg gaacggcgcc gcggcgatct acaacctgat
     3841 ggaggacgcc gcgacggccg agatctcgcg cgcccagctc tggcagtgga tcaaccggaa
     3901 cgccacgctc gccgacggcc ggactgccac gaaggatctc taccggcagg tgcgcgacga
     3961 ggagattccg agggtgggag ggatcgcgaa cggccggatg agggaagccg tcgagatcct
     4021 cgaccgcctc gtcctcgacg acaccttcgc cgagttcctc acgcttcccg cgtacgccta
     4081 cctcgagtga gccgccgcgc ggggggcgca aggccccccg cgcggccgga ctttcatcag
     4141 gggttcagga atccgaggtc ggccgtcggg aaccggtaga ggttcgggaa gaccgtattg
     4201 agctcggccg gcgtcgcgcc gagccacagg ccgagcgtgg ccgcgtactg gtcgacggcc
     4261 gtgctcggga tccagcggcc gcggctgtcc gtgtcgtact gcgacccgag ggtgaggtcg
     4321 gggaacggcg tgccgttgag gccggcgttg ccgtagaagt cggccgcctt gatcgcgccg
     4381 cccatgacga gctggtgggt gccccatgcg tggtccgaac cgacggacgc cccggaaccc
     4441 gacggcgcca tcgtgcggcc gaagtccgac atcgtgaacg tcgtgacgtt gttgtggagg
     4501 ttctggttga acatctcctg gtagaacgcg gccatcgcct tcgagatctg cgtgtagagg
     4561 ttcgtgtggg acgcgagctc gtcctggtgg gtgtcgaagc cgccgatcga gcagaagaag
     4621 acctgccggc tcacgttgag agacacctgg ttctgcttga tgatcttggc gacctgcttg
     4681 agctggttgc cgaggccggt ggccgggaac gtggccgtca cgaccgggtc cgtcgcgaga
     4741 gacgcctgga tgtcgaggcc ctgctgcgtg atgtcgctcg ccgccttcac gagcgggact
     4801 ccggtgtcga aggtccggag cgtgttgaag gccgtcctgc gcgcgttgtc gatcgcgttg
     4861 ttcgcgaagc cgttgagcgg gaagatcgcg ttcagcgccg tcggcgcggg cgcgacgttc
     4921 agcgccttcc ccgtctggcc gacgttgaac tggttgttgc ccgagaggga gagggacgcc
     4981 gggaaggacg tccccgtgtt caggtcggaa atcatgtcga ccacgcgtcc gccccagccg
     5041 tagatggact gtacgtcgga ctgcggcgcc atccaggcgg actgctggtc gctgtgcgag
     5101 aacagctggt acgggcggta ggccgcgttc gactggtaga gcgccttcgt cgtgggccag
     5161 cgcgtcatcg gcccgacgtt cgagacgacg gcgagcttgc cggcgttgta gagcgcggcg
     5221 agctcgggca tcgcaggatg caggccgaaa ttgaggccga agctcgtcgg gctcgtgaag
     5281 gcggtcgccc ccagcggggg aagcgcgagc gacgctccgc gcgacgtcgc gtactggttg
     5341 tacccggaaa ggcccccggc cgtgaccggg atgaccatgt tgttcgcgtc gttgccgccg
     5401 aacatgaaga tgcacacgag ggcgcggtac tggttcggcg gcgccgcgga ggcggcgtgt
     5461 gcgaggaggc cgaaccggtc gaggccctgt tcgaaggccg cgaacccggc ggcgccgcac
     5521 gcgagcttga ggaattccct gcgattcgtc atgtttacct ctcgacctgg aactgcgagg
     5581 acgtggcgac gagatacacc gcctgctgcg cacgctgctt ggggctcgtc gacggcacgg
     5641 cgttgacggc ggtgacgatc gccgacttcg cgcccgcgga gagctggccg cggcagagga
     5701 gctggttgag ctgttcgacc atcgcggccg gatcgttcga gagcgactgg atgacgctca
     5761 gatcgagcgc cgttccggag gggttgttcg cacccgtcgg gatggtcgaa tagacgagcg
     5821 tgttcacgag gttcgcccgg cgaagcgcgg tgagcgcgga ctggatgccg aattccggcc
     5881 cccacatggt cgacgagccc ggcagcctgt tgtcggccgg gtagtagccg aagaccgtgt
     5941 ccgggttgaa gaggttctgc ccgaggttcg cgagctgcgg cgcgaggacg ccgtccgaac
     6001 gcccggagcc gttcgccgcg gtcgcgccga gcccccggag gagcgcgatc acgtggtaca
     6061 cggggtcgcg gaggatgccg aagttcgggt ccgcggcgcc cgcgagcgcc tcggaatccg
     6121 tcacgatcgc gcggacgacg gcggcgaggt cgcccttcac gcccgagccg ttgttgttga
     6181 acaccgccgc gacgcgcccg acgtacgccg ggctcgggtt ggacgtgacg aggccgtgga
     6241 tcagctgcgt cgagacgtac ggcgcgacgt tcgggtgcgc catgatcgcg tcgatcgcct
     6301 gggcgacgtc cacgtctccg ttctggttcg cgttgaaggt ccaggcgcag gccgtcggcg
     6361 ttccggtcga ccagtcgcac aggaaggact tcttgccccg gtcgtggctc gcctcgacga
     6421 ggaccatgtc cgaggtgtag ttcgacgtcc cggccacggg gggcgccgcg agccgccagc
     6481 cggtgaagac gcgggagagc tcgttgatgt cgttctggtt gtacgtcggg atcgtgttgc
     6541 cgccgaggtc gagcttcggc gtcccgtccg tgttgagctg aacgaggccg atcgagaaga
     6601 gctgcaggat ctcgcgcgcg tagttctcgt tcgggttctg gagggtcgag gtcctcatgt
     6661 tgaggtagtc gcccatcgcc gggttcaccg tgagctggta gaggatgtcc cggtagtgtc
     6721 cgaacgcgtt cccgtagagg atgttcaggt acgggatcat gtggctcggc tggatctcct
     6781 gctggcccga gaccacgaga aacttgtgca gggcccagaa gacgcgcatg cggagctggt
     6841 cggggtagta gagcgcgttg tagaggaacg tgctctggag cgggtacatc gtgtagttgt
     6901 cccgctggca ggtgcccgtg caggcgcccg gaatcgtgtc cgggaacagc gccgccggaa
     6961 cgaagtagtt cggcggaatc gagaactgct cgttcagcca gccgttcacg ccgacgtcgc
     7021 ggatgtgcgc gacgagctgc ggcgtcgcgc cgaagctcgt gaggttcgcc accgcggtgt
     7081 aggtggacag cggcgagggc ggcgggtctt cgaagtagcc cgagacgtcg atgaggaagt
     7141 gcacggtgcc ggacggcatc tccgtgaaga cggcgatgtc gcccgtcgtg ccgaggcggg
     7201 cgatgaagtt gttcgcgcgc gtccggccgg ccttgaagtt gatcgccgtg gcgcccgtcg
     7261 gcgacggcgt cccggtcggg aagatcgcga ggtcgcccgc gctcgacggg ttgaccacgg
     7321 tcaggttcgt gacgacggcg cgcgccgtca cggggacgcc gcacgatcct gcgagctgga
     7381 acgcacgcgc gccgcccgca gcgagcgccg gaccgccgga ggcgccgttc gggttccgcg
     7441 tgtcgaccgc ccggcagggc gtgatcgggt agaacttcga ctgtggcgac tgggccagaa
     7501 ccgcccccgg caggagcaac gacaggaggg cgacagaccg ggccgaaagg caatacttca
     7561 tcccgtactc ccgcggatta accgcacgtc gatggaatct gttcagttct gccggacgtc
     7621 cggctgaatg tgacctggat ctctaccggg gagtgtcggc acctatcgcg gagaacggcc
     7681 cggcgccgaa cgcgttgcgg cgcaacgact tagctgagac ggcgtctcgg tccgtgtgtc
     7741 agacggagga ccgggcggcg gccttcgacg ccagaaagtt cgcgaacccg cggtcctggg
     7801 tcctcacgtg gccggaaagc cacgagcgca ggccgtcgac ggccacgtgg gtcatgggca
     7861 gcgccccgct ctcgtacgcc gcgcggagct tctggagctc ggcaaggagc tcgccgtgct
     7921 cgccgcggtg ggcctcgtag ccgggatagc cctccatccg catcatgagc tcctcggcga
     7981 ggaagtggac gcgcgtgtag tcgagcagct tgtcgagaat ctcgtcggcg acctcgcggc
     8041 tcttgccttc cgcgaccgca cggccgagcg cctcgacgag ctcgacctgg agcgcgtgct
     8101 cggcgtcgat tgcgggaacg tcgaggcgga ggtcgcggtc gggcgccacg ccctcacgat
     8161 atcaaggccg gagcgtggcc cggtaccagt cgaccgtctt cccgaggccc tccgagagcg
     8221 tcgtcctcgc ccgccagccg aagagaccgg cggcgcggga gacgtccagg cgccgcctgg
     8281 gttggccgtt cggccgcgac gcatcccaac ggatcgagcc cgcgaagccg atcgcgccgg
     8341 cgacgcgctg cgccaggtcg cgaatcgcga tttcctcgcc ggagccgaga ttcacgggct
     8401 tggccccgtc gtacgcttcg gcggccgcga cgatcccctc ggccgcgtcg tcgacataga
     8461 ggaactcgcg cgtcggggaa ccgtcgcccc agaggacgac ctcgggggcg ccggcggcct
     8521 gcgcctcgac gaacttccgg atcatcgcgg ggatcacgtg gctcgtcgcg aggtcgaagt
     8581 tgtcgcgcgg gccatagaga ttcacgggca ggaggtagat cccgtcgaag ccgtactcct
     8641 gccggtaggc ctggagctgg acgaggagca tcttcttcgc gagtccgtag ggcgcgttcg
     8701 tctcctcggg atacccgttc cagagatcct cttcgcggaa cggcaccggc gtgaacttcg
     8761 ggtacgcgca gatcgtcccg agacagacga acttcgcgac gccggcgcga cgcgcctcct
     8821 cgatgagctg gatccccatg atcgcgttct cgtagaagaa ccggcccggg tgggcgcggt
     8881 tcgcgccgat gccgccgacg acggccgcgg cgtggacgac gacgtcaggc ttctcgcgcg
     8941 tgaggaacgc gcggaccgca tcccgctcac gcaggtcgag gtcggccgcg cgcggcgcga
     9001 ggacctcacg ggctccgcgg cggcggagcg cctcgacgag cgcctgcccg aggaaacctc
     9061 ctcctccggt gacgaggacg cggcggcccg accagaaggt catgtcctcc ggagtgtaaa
     9121 tcccgtgacg cgtgtcatcc tccgcggggc ccgctcccgc cgagaatccg ttggaaatcc
     9181 ggcgcggcgc cactccccgg ggacggcggg cgcggcccgc ttcttgcgac tggctctcga
     9241 gaggaatttc gatgaagact gccttacttc tgcgctccct cacgttcgtc gtgcttctgt
     9301 ccgcaacgat gtccccagcc gacgtcgtga tcccgtccag cgtgtaccgc cgcggcgcgg
     9361 gcggagcgga gttccggagc gacgtccggg tcttcaaccc gacgacgtcc ccggtcaact
     9421 acacgcccgt cctctacgac caggtcaccg gccagacgtt cacggccgcg atccagacga
     9481 tcccgtcccg gacgcagcag tccttcgaca acatcgtcgg aacgctcttc gggcgcacgc
     9541 tcgacaacgg ctcgttcgga ccgatccggt tccagacgtc cggcacgctc ctcgtgtcgt
     9601 cgtccgtcaa caactacaac gcgtgcggca acggctcggt gtcggggcag tggctccccg
     9661 gcctcgacgc cgccgcggcc ctcaaggcgg gcacgctcgt ccagctcgcg tcgagcgtgg
     9721 acctcgcggc cggctaccgg acgaacgtcg tgttcacgaa cccgggctcg tcggacgcga
     9781 ccgtccgggc gaaagtgcgg cgcggcgacg ggacgctggt ctcggacgcg acgctcccgg
     9841 ctctcggccc gaacgggttc acgcagatcg ggaactgggg cccgccgttc tcgggcgtcg
     9901 cgggcacgaa cgacacgaac ttgtggatcg agttcaccag cgaccagccc gtcctgtcgt
     9961 tcgcgtccgt gatcaacaac gcctccggcg atccgttcgc gatcgtcatg acctcggagc
    10021 ccgcggcgcc cccggcagcg cccgtcgcga actacacggt atccgccagc ccccagccca
    10081 accagcccgt gaccttcacg gacctctcga ccggcacccc gacgaaccgc ttctgggtct
    10141 tcggagacgg aacgttcgcg acgaaccctg tcgcgcccgt gcagcacacg tacacggcgg
    10201 ccggcacgta caagagcgcg ctcttcgtgg acaacgccgc gggcgcgagc agcaagacgc
    10261 aggacgtcgt cgtcgcatcc gcgacgccga tcgcggtcac gatctcggcg acgacgacga
    10321 acaacacgaa gtggacgttc gtctgccaga cgggtccctg cagcggcgcg gggaacaaca
    10381 acgtgaacct caaggtcggc cagccctaca cgatcacgtg gacgacgccg gcctcggagg
    10441 cgaagacgca cggcgtcggc ggcatcgcgg tgctcaacat cacgcagtgc gacgtcatcc
    10501 ggtccaacca gccctgcacc gcgagcctca cgccgacggc gtccatgctg aacttcccag
    10561 gcccggtcta cacgtacttc tgcacgcagt cggcctgcgg cactcagccc cagcacgacg
    10621 gcatgtcggg cacgatcacg atcgtcccct gagccctcct accttctagg aagtccccgg
    10681 gcggccgcga gagcggccgc tctttctttc taggtctctc tctcggccca gaggcactca
    10741 cgcgggtcct tgtcctctcg caacccgagg aacgacggct ggcggagccg gagttcgccc
    10801 gtccattcgg cgaaagcgac ctgcgcgacg acctgcggct tgacccaggc cgcgtccttc
    10861 acgccgtgag gaggcggcga gaacggacac gcgtcctggt gcagcggctc gagcttttcg
    10921 aggagcgaga ggaggatctt gtcggaaaaa ccggtcccga ccgcgccgca gaagcgcagg
    10981 tgcttccctt cgtagaggcc cacgaggagg gccccgaagt gcgggcgcgg atcgctcggc
    11041 agcgtgagcc cgccgatcac gaactcctgc tcgcgccgga ccttgacctt cagccagcgc
    11101 agcgagcgcc ggccggactc gtagggcgag gagaggtcct tcccgacgac tccctcccat
    11161 cccttcttca ccgccttcgc gaacgcagcg gcgccgtccg tcccgagccg ctccgcgagc
    11221 gcgagggggg gtgccgggtg cttcccgacg agcgcctcga gagcggcccg gcgctcgccg
    11281 agcgggagtg acatgaggga gacgccgtcc gcatcgaggc agtcgaacag gacgagcacc
    11341 ggaggcctcg cgcccggctc gttccttgcc tggagaagct gaaagctcga cacgccccgg
    11401 tcgtcgagca cgacgagctc gccgtccacc gcgaggttcc cgccgggcag cgcctcgacc
    11461 gccttgacga tgtccgggag ctcctcggcg atgtcgaatc cccggcgcgt ggcgagcgtg
    11521 acccggccct tccggcggat cgcgacgatg cgccagccgt cgtacttcgg ctcgaagacc
    11581 catccgcgcc ggctgaacgg ctccttcacg cgcgtcgcga gcatcggagc gagaggcgag
    11641 aggggcctca cggcgccatt ttcatccgaa cagccggtcg atcggcgcgt agacgcccgc
    11701 gcctgcgttc gggacgtact cgaagccgcg gccgatcgga tcgtcgcggc gcgtcgtcgt
    11761 ccagtcgatc ccgagcgccg agtagacggt gcacgcgagg tcctcggcgc ggagctccgt
    11821 ccggcccgtc acgcccgagt ccgtgatcgt cgcgcccgtc gcgtccgtcg cgccgatgac
    11881 ctgcccgccc ttgacgccgc cgcccgcgaa gaccgcgcag tagttcgcga agtggtcgcg
    11941 gccggccgcg tcgccgagcg gcccgaccgt ccggccgaat tcccccacga cgaagaggag
    12001 cgtctcgtcg aagagcgtct tgcccgccgt cttccccggg gtcgccttga ggtccccgat
    12061 caggctcgcg aggcccgcgt cgagctgcac cgcgaggctg tagagactgg tctcgccgag
    12121 gttcgcgttc acggacttct tgtagatgct ggcgtggtgg tcccacccgc ccaggccgag
    12181 catgacgaac cgcgtgccgc ggccggccgc gagcacctgt ttcgcgagga ggcagctgtc
    12241 gccgaaggcc gagctcccgt agcgcgccga atccgccgcc gcgatcgtga ggaactggtt
    12301 cacgtcgggc gaatccacga ggagcttggc ctggcgatag aggtccgggg cgtccgcggg
    12361 ctccttgccc atcggctggc ccgtccggag cgccgcgtcc agctcctgca tccaggcgta
    12421 ccgggtcgcg agcgcggccg ccccatccgg atgggtgagg ttcgggagcc cggtcgcgat
    12481 cggctgcgcg acgaaaggag cgaccgtgga ggggaggtag ccctcgtcgg cgaggcccgc
    12541 cgtgcccgtg ttgaaggcga agaagcccgg gaggacgtcg ttcggcccgc gctgcttctc
    12601 gagctcgagg gccacgacgg acccgatgtt cggggcgatc gcgcccgtcg cgcccgtggg
    12661 gttgcgcgcg atctggagcc aggtctggtt gagcccgtgg acgagcgccg ccgatttgag
    12721 cgagcgcacg aacgcgaggt ccccgatccg gttcgcgagg ttcggcatga gcccggccgg
    12781 gaacctcacg ccgcccccga agctcgtcgg cgcgaagtcc gagggcgtcc acgcgccttc
    12841 cttcagatcc cacgtgtccg tctggctggg cgcgcccggg aggaagacga agatcgcgtt
    12901 ccgcgccgtg cctctcgtcg agaccttcgc ggaccgcgcc gcccacgcgg cggccgggga
    12961 gccgaggaac cacgacgcca cgaggcctga gccgccgaga cgcaggaagc cgcggcggga
    13021 gaggtcggcg cccgtgaccg gcgtgacgcc ggggaccggg aggctcggga ggagcgtctt
    13081 cttcatcagt agttgtagag aaagtcgagc ttctggagga ggacgaactg gagcgtctgt
    13141 gcgaaggacg gaagcgacgt tccctgggcc ttcgagaggc accacgcctt ctcggcgggc
    13201 gtcggaaccc gagagagcgt cttcaggaag agcgtgtcca cggcctgctc gggcgtcgcg
    13261 ttcgccgcga cgaggcggtg cacgaccgaa tcctgggtgt cgtgggaccg gatcgtgacc
    13321 tggttgttgt tcagcatcgt gagggcctgc gcgaccgaga acttccacga gcgcgggacc
    13381 gtgtcgcggt tgccggggcc gaacgcgctc atgaaccggc cgttgaggtc cggcccgttc
    13441 tggaacccca tgttcgggag ctggagggcc agggccggcg agccgctcac cgtcatgttc
    13501 gaggggacgt tcgtggcctt ctgaaccgcg tcccagagct cctcggcccc gagccgccgc
    13561 gcgaagtggc gcgcgaaata cggcgtgtac gcttcgctcc acgtccccgg atagaaggac
    13621 gagagctggt aggcgttgga cttcgccatc acgccgagga tgtaccgcag gtcgtacccg
    13681 tgcgcggcga agtcctgcgc gagccgattg aggagagccg ggtgcgtcgg ctggacggtc
    13741 cacgcgccgg gcggcgggct ggccgggtcc tgacgcatcg ggtcgaaacc gtccacgggc
    13801 tcgacgatcc cgagcttgaa catctccttc cagagagtgt tcacggccgc gcgcgcgaac
    13861 tgcgggtcgt ccgtcacgag gcgcgcgagc cactgccgaa aggtctcgcc gggcaggatc
    13921 ccgcccgggg acgggttgag cggcgttctc gggtacgccg ggctgatggt ggtcaatccg
    13981 ggcggcgtcg tcgtgtagta cgcgcccgtg cggtccgtct tgttgccggt cgtcgtgttg
    14041 agccggtagt cgtagttgcc ggtccgacgc tggccgatcg tgtacaccgt gttgggcaca
    14101 ggccccgcga acggcatgtc cgtccacgag aagaacgcgg ccagcgacca gaagtcctgc
    14161 cgtttcacga tcgagcccca gaggttgatc gcgtcgaggt gcccctgccc gttgtgacag
    14221 gacgtgcaga agacgacacc cgtcccgagg aagacgcgcg aggtcgcgac cgcgaggttg
    14281 tcgtaggtgt cctgcggggg accgttgttc tggaactggc gcacgatgaa gttcgccggc
    14341 cccacggtcg tgttcgggcc gctcgccgtg atgagctccc gggccatgac gtcccacggc
    14401 ttcctcgagc gcacggcgtc ctggaagtac gcgtggtacg cggcgttcgc ggtatcccaa
    14461 agcgtgcccg tcgagctgcg attcgtgttc tgcacgagct cgtcgtagaa gaacgtccag
    14521 cggtcgacga aggcgtcggt ggtgaggagc atgtccacca tccgcgagcg cttgtcggac
    14581 gacggatccg cgaggaacgc gatgaccgtg tcggcgtcgg ggatgcggcc cgtgaggtcg
    14641 agggtgacgc gacgcaggaa ctcggcgtcg gaagagcgcg gcgccggcgg gacgcggtcg
    14701 gcttccatct tcccgaagat ctcgtcgtcc acgtagttga cgcgcttgag gcgcgaggcg
    14761 gggtggaacg ccgtcgacga ggcggccggg tgcgcggacg cgaaggcgcg cgtgtcgcgc
    14821 gaaacggcct cgcggcggcg gcggccgtcg gggtcggcgt gcgacggcac gaacgcgcag
    14881 tccgaagtgg cgggccgaac ggttccgtcg gccaccatgg cctcggcgcc gcccgtctcg
    14941 acggcctccg caggcctgcc gggagggacg atcgcgaccg ccagaaagag gagatccctt
    15001 acgcgaagcg ccaggcgccg agcggcgccg cagcaacccg tcctgtttct cattcgcaga
    15061 aatcctacac gattctccgc gaaagagtct caggccgcgg gagggaaccg tcagccaaac
    15121 aagcgatcga tgggcgcgta aacgcccgcg gcggcgttcg gcacgtactc gaatccgcgg
    15181 cccagcgggt cgtcgtggcg catcgtcgtc cagtcgatcc cgagcgccga gtagacggtg
    15241 caggcgagat cttccgcgcg gagctcggtg cggcccgtga cgcccgagtc cgtgatcgtc
    15301 gcgcctgtcg cgtccgtcgc gccgatcacc tgtccgcccc tcacgccgcc gcccgcgaag
    15361 acggcgctgt agaccgcgaa gtggtcgcgc ccggattgcc cgttgaggtt cccgaccgtc
    15421 cggccgaact cccctgccat gacgatcagc gtctcgtcga acagagtctt gcccggcgtc
    15481 ttcccgggcg tcgccttgag gtccccgatc aggctcgcga gcgccgggtc gacctgcccc
    15541 atctgcgagt agaggctcgt cccggtcttc gcgtagatgt tgctgtgcat gtcccagccg
    15601 ccgaccgaca ccatgacgaa gcgcgcgccg cggccgcccg cgaggagctg cttggcgacg
    15661 aggcaggcgt ttccgaagga cgagttgccg tagcgctggg catccgccgc cccgtacttg
    15721 aagaggtcgt tgacggccgg cgtgtcgatc atcaccttgg cctggttgta gaagttcacg
    15781 gcgtcggccg cgtccttgcc gaggggctgg cccgagcgga gcgccgcgtc cagctgcgcg
    15841 aggtcgttcc agcgcgtccc gaggcgcgtc gccccgtcgg gatgcgtgag gctcgcgagc
    15901 ccgttcgccg tgggctggac gacgaacggc gcgtacgtgg acgggaaata ccccgctccc
    15961 gagaggcccg cctgcgtgtt cagcgacagg aacgccggga gcacgtcgtt cggtccgcgc
    16021 tgcttctcga gctcgagcgc cgcgacggcc cccatgttcg gcgcctcggc gcccgtcgcg
    16081 cccgtggggt tacgcgcgat ctggagccac gtctggccga ggccgtggac gagcgccgca
    16141 gacttcatcg agcgcacgat cgacaggtcg ccgagccggt tcgcgaggtt cggcatcagg
    16201 cccgcgggga accgcagccc gcccccgaag ccggtgggcg cgaagtccgc cggcgtccac
    16261 gcgccctcct tgaggtccca cgtgtcgacc tggctcggag cgcccggcag gaagacgaag
    16321 atgacgttct tcgcggtgtt ccgcggcgtc acggccgcgg actcggccgc ccacgccgtc
    16381 gcgggcgact tcaggaacca cgaggcgacg aggcctcccg caccgagctg caggaagccg
    16441 cggcgggaga ggtcgggccc gttgacgggg gtcgcgccgg aagccgggaa gctcgggagg
    16501 agcggtttca tggcggccct cagtagttgt agaggaagtc gagcttgttg aggagcgcga
    16561 actggaggtc ttcggccacc tgggccttcg tctccccgtt cggcgggctc gcgaacagcg
    16621 cgacgcccgc ggcgagttcg gccgccgagg ggctgcgcga cagggtcgag aggtatagcg
    16681 acgtcacgat ctgcgtcggc gtcgcgttcg ccgcgacgag cttctgcacg gcggatcccg
    16741 ccgtcgcgct cttgatgcgg ctcgtgacgg tcgtgtcgtt cagcatgagg agagcctgcg
    16801 acaccgagaa ctcgttcgac cgcgcgtccc cgtcgcggtc ggcgcgcagg aaggcgttca
    16861 ggaaattgcc gatcacggag ccggccccag gctcctggac gtcgggcagc tgcatcgcga
    16921 gcgtcacgtt cggcatgccg ccgctcaccg ggatgctcgc ccccacgttc gtcgcgcgcg
    16981 tgatcgcgtc gaacatttcc tcggccgtca gccggcgcgc gaagtgcctc gcgaagtaag
    17041 gcgcgtacga ctcgctccac gccccggggt agaaagacga gagctgatag gcgctggact
    17101 tcgccatcag tccgaggatg tagcgcaggt cgtagccgtg cccggcatag tcctgggcga
    17161 gcttcgtcag gagcgccggg tgcgtcggct gaagggtcca cgagccgggc ggggggctcg
    17221 ccgcgtcctg ccgcatcggg tcgaatgcgt ctgcgggctc gacgattccg acgccgaaca
    17281 tctccttcca gagatagttc acggcggcgc gcgcgaactg cgggtcgttc gtgacgaggc
    17341 gcccgagcgc ctgccggaag ccttccccgc tcgccggcgc gccgcccgcg ggggcgagcg
    17401 gcgtccgaag gtacgccggg tcgatcgacg tcatccccgc aggactcgtc gtgtagtacg
    17461 ccttcgtcct gtcggtcttg tttcccgtcg tcgtgttcag gaggtagttg ccggttccgc
    17521 gttcaccgac ggtgtagtaa tagttcgcgt tggccgtccc ggagcgcggc gcggtcgcgc
    17581 gcgagtagaa cgaggccagg ccccagaagt cctgccgctt caccgtcgag ccccacaggt
    17641 tgatcgcgtc gaggtggccc tggccgttgt ggcaggacgt gcagaacacg gcgttcgtcc
    17701 cgaggaagac ggccgccgtc gaggccgcga ggttgtcgta cgtgtcctgg atcggcccgt
    17761 tcgcctggat ctggcgcacg acgtagttcg cggcgccgac cgtggtgttc acgcccgtcg
    17821 cgccgatgat ctcgcgcgcc atcgcgtccc acggcttgtg cgacctcacg gcgtcctgga
    17881 agtacgcgtg aaacgcgttg cggccctggg gatagagccg gccgctcgcc gcgaacgcag
    17941 tgttctggaa catctcgtcg tagaagaacg tccagcgatc gacgaaggca tcggaggcga
    18001 gaagggtgtc gatcatccgg ccgcgcttgt cgggcgccgg gtcggcgaaa aacgcggccg
    18061 cggtcgcggc gtccggaatg cggcccgtga ggtcgagcgt gacccggcgc aggaattcgg
    18121 cgtcggaggc gagcggcgcc gggacgacgc gcgccgcgtc catcttcccg aagatctcgt
    18181 cgtccacgta gttggcccgg cggaggcggg ccgaaggctg gaacgcgggt ccggccgcgg
    18241 cgggatggga cgacgcgaac gcccgcgtgc cccgggaaag ggcctcgcgg cgaaggcggc
    18301 cgtcctggtc cgcgtgctac ggcacgaacg tacagtcggc ggcc
//
LOCUS       DAOMFY010000050        18314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_954_length_18314_cov_7.198970, whole genome shotgun sequence.
ACCESSION   DAOMFY010000050 DAOMFY010000000
VERSION     DAOMFY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_18314_cov_7.198970"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="PLB01_06250"
     CDS             complement(<1..1484)
                     /locus_tag="PLB01_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ76937.1"
                     /translation="MNGVSGCGSGGSASEVPLQPSKNSPSLSSSSVPALELRLISKRF
                     GETQALSEANLVVFQGEIHAVNGAGKSTLVSIAAGRLAADSGEILRGGRSVRFAGARD
                     ARDAGLALVPQHDLLVGAASVADNLALLDRGAPLVESGRARRARVARLAETFGLDLGD
                     PGDRVDTLPVGTRQRIEIAGALAGEPDVLILDEPTAVLSPDETAALFAALRKQADGGA
                     AIVLITHRLAEVFAGADRLTLLARGETVKTCRIEETTAQEIGGLLIAGSGTEESENEE
                     EREKKIFLEGEEGGEPVEGRRGDARRTGATRPSKNAARSAAGLREGSSNLSLSRFAPR
                     GSQGKQELLSLSLSPGELLVLLAIDGNGADSIASAIAGLTPFTGEVRVGGAALPPSGD
                     PLAFRAAGGAFVPADRRDEGLVLDLDLAENLALPDPPGRLLLDRAAMRTHALERMEAF
                     AVRAPSPSTRAGALSGGNQQKLVLARGLARSPRLVVAIHPTRGL"
     gene            complement(1568..2554)
                     /locus_tag="PLB01_06255"
     CDS             complement(1568..2554)
                     /locus_tag="PLB01_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HQQ76938.1"
                     /translation="MSRRSVRPSAACAAALALAACGGPRETPKAPAPALTVALLTPGP
                     ISDAGWNAGAYEGLKAIERELGAGVSHVQVKSPSEFEEQFRAYAAKGTSLVFGHGFEF
                     QDAAERVAKEYPKTVFVTTSGSRTAPNLAPIVFELEQAAYLCGLLAGRTTRTGKAGMV
                     GGVELPSIKSTFLAFEGGFKAANGAGTATSVFTGSFEDVAGAKAASLALADKGADSLF
                     QNADAAALGVFKAAEERKIAAFGTNKDQNALSPSILASAVIDMPRAFVETARRVKAGT
                     FDGKPLRYGLGSGVVSFVLSPTANIPPAVRDELKAAEEKIRAGTLVVPRGNF"
     gene            2588..3184
                     /locus_tag="PLB01_06260"
     CDS             2588..3184
                     /locus_tag="PLB01_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76939.1"
                     /translation="MALVLDTASKARILDFVRPLSVGLDGMTNYGFVERRIRISDFLE
                     GEEGRGEAPDGARADPDRLFLLCAFAGLPSARRITPDSREDLLLRSLRLPLEEIRILV
                     SSLSRLESSPETPEEKIVHDAMLLESVGAYGVTQILVAGVRERMTLAEMADEIDARMD
                     AARFVTEAGRRLAGERIAYAKDFAAKLRAEAAEFETPS"
     gene            complement(3186..4442)
                     /locus_tag="PLB01_06265"
     CDS             complement(3186..4442)
                     /locus_tag="PLB01_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76940.1"
                     /translation="MQALASLACLVLLAVQPASPTTPKLTLVSPKGGESWVLGTTQNI
                     VWTASGVTGEVRISLQQEGWNLGRGLIAKVPAGTGSFAWTVGKLMPGYTSLTEKGTGF
                     RIRIEALNPTVIGESPQPFGIAEMPTARPNVALAPGALAPKTITVTSPAAGATWQRTQ
                     TYDITWQQSENLLKEFARILLLRSTGEISLTIETKRPLQSGRHSWQLPGVNDVPALTE
                     GDYRVRIETVNGEAKGDSGLFHVARTVKFSNPILPATTKNGSHWFASTGKQESSTWGP
                     VEPGAGTVKVGFINDYNKPKQVSVTYRSHIFVDLKGVKGKVKWAKLSYEKNDGTPDGA
                     RPLWVITQPWNGTSMDMWNHPGNAVNLSDAGQMKAIVQGWIDDPTMNFGFLMAGPDES
                     MARAAKTGHVMILSNVRLDVGIDAVY"
     gene            complement(4532..5275)
                     /locus_tag="PLB01_06270"
     CDS             complement(4532..5275)
                     /locus_tag="PLB01_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ76941.1"
                     /translation="MKVALVTGGAKRLGFEFAKALAKNGYSIALSYRSDPEEAGRAVD
                     FLRKEEGVRAEAFRIDLAVDGAPIKLFEELFSSPLFSPPSSPPRLDVLVHAASPWIPK
                     PVAAVTLDDWEAVFRAGPRAAFFLAQAAAPELARSSGSILFVSDVAATQAWPTHVPHA
                     AAKAAINALVRNLAVALGPNVRVNAIAPGVVMPPTDMSREAVDALVAKTPLGRPVAVE
                     DLTSMAVAILENRSMTGQVVAVDAGRSAA"
     gene            complement(5272..5784)
                     /locus_tag="PLB01_06275"
     CDS             complement(5272..5784)
                     /locus_tag="PLB01_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76942.1"
                     /translation="MALALTLLSLFTLAALGLSAWSGWTLSHALGGLSAARHIVVAVP
                     AVLFSLFTQSMVLFFFIGTGKLLKEAAAKRPDAEGRDYILRRVKDFKARTSALATFAP
                     LSALAAGLLGAGAHTGATPAWLHLAAAILTVLLHAQALVREVVAMAETNKLMDEAATL
                     VPPAAGGDGA"
     gene            5838..7640
                     /locus_tag="PLB01_06280"
     CDS             5838..7640
                     /locus_tag="PLB01_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQQ76943.1"
                     /translation="MTPRTLVDIFRNLEKNPKPDLLLVKKSGAWTPISTAQFMDSVKA
                     VSCAFEGLGVKPGGRVALLSENRPEWAIVDFACQCYGAVLVPIFPTMVSDQVTYLLKD
                     SGATVAFASNEDQARKVLAARAASAEVNASLQHVFSLDTSTLGGVEPFTDVLEKGKAA
                     HAADPAAFEKRADARTPDDLATFIYTSGTTGEPKGAMLTQQNFVSNVLAGCSILPLDS
                     SCVALSFLPLAHVFERMLEYVYYHRAATIAYAESIDKLRDNMGEVHPHLFGAVPRVYE
                     KIYARVQENLAKAPAGKQKLFAQAIDVGKKVVALKAKKQTPDFALALQHFVFERLVYR
                     KVRAALGTRFRYAVSGGAPLAKELAEFFWAIGVEIYEGYGLTETSPVIAVNCPSAWRL
                     GAVGKILPGVECRIAPDGEILTRGPHVMKGYYNKPKETAEAIDSEGWFHTGDIGVIDA
                     DGFLAITDRKKEILVNSNGKNIAPAPIESFLKSQDFISLPVIIGDKRKFLSCLIIPNF
                     EKLAAWAEANGFAGRPMEQLVKEPRVLALFQTAIDRWNEGKAHEQLVHRFALLPKDLT
                     IEDGELTPTLKVKRRIVDQHYKHLIDEMYEGSGA"
     gene            7660..8619
                     /gene="arcC"
                     /locus_tag="PLB01_06285"
     CDS             7660..8619
                     /gene="arcC"
                     /locus_tag="PLB01_06285"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HQQ76944.1"
                     /translation="MRVVVALGGNALLRRGEPMTAENQRANVRRAAEALAPVAREHEL
                     VISHGNGPQVGLLALQAESGLDAAHFPLDVLGAQTEGMIGYMIEQELGNLLPFEKPFA
                     TLLTMVEVDPADPAFRNPTKPIGPVYSEEDARRLAKERGWAVAPDGGKWRRVVASPQP
                     KRLFEIRPVKWLLEKGTIVICGGGGGIPTVYGEDGKLHGVECVIDKDRVASLLAQQLD
                     ADFFVMATDVPAVSVGWGTPSAKAIRRASPAAIGRFDFASGSMGPKVEAARDFAERTG
                     KTAAIGALEDIPRILAGEAGTLVSNGVATIEWAAAESLSLFTE"
     gene            complement(8609..9124)
                     /locus_tag="PLB01_06290"
     CDS             complement(8609..9124)
                     /locus_tag="PLB01_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HQQ76945.1"
                     /translation="MRTRRALPFAFVLAFSPFLPGCATILNGPNQTIHVETDPPGATV
                     RVAGETHTSPVDLVLKRAAKGLVVVIEKEGYVTRKVPLERGASPNTWINFVGLPAGLV
                     AGAAIGAQNSEGLDEISSGATGLAIGALVVPGAMFAVDAASGSIHRLDPPLIAVKLEP
                     APPPAALPSTP"
     gene            9269..9991
                     /locus_tag="PLB01_06295"
     CDS             9269..9991
                     /locus_tag="PLB01_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ76946.1"
                     /translation="MMAHSSGREGLGTDAAALAAAPEKAFPGADPEKRRRILEAAIRT
                     FGRRGFHEARIADIAADAKVAEGTVYLYFRNKEDLLGVVFDESMDDVLEKGRAVARSD
                     APAAERLTALLDLHFQFIGSDRDLASVFQIELRRSARLVERFSRSKLVEHFRLLGDVL
                     RDGIARGEFRRDLDPRLAVRILFGAADEILSEWLLSGEKKPSADAKQLVGTLLSGFAQ
                     TREREKKRISLKVKKANRSNPK"
     gene            9988..11244
                     /locus_tag="PLB01_06300"
     CDS             9988..11244
                     /locus_tag="PLB01_06300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQQ76947.1"
                     /translation="MSKTAFIVGGARTPFARMGTDYTRVSAVDLGRAAAVEAMARSGI
                     EPSAVDQSIFGNIATPVDAANIARIIALRAGVPKERPAHSVSRNCASGIESVVEAARL
                     VAIGEADVVLAGGVENMTQIPFLYRDSVKGVFAKAGMAKTAGQRLGALSGMPWKDLLN
                     PVIGLKEGLNDPVCDLGMGDTAEILAKEGRITREEQDHFALRSHQRATEARAKLALEI
                     VPVPVPPDYDRVLTFDNGVRENQTMEALAKLKPVFDRKYGTVTPGNASQITDGGAAVV
                     VASEDFVVRHGLKPLGRLKSWGFAGLEPERMGLGPARSTPLALKKAGLSMKDIGLVEM
                     NEAFAAQVLANLREFAASKELGEIPMDILNVNGGAIALGHPVGCTGTRLILTLLLEMQ
                     RREVRHGLATLCVGGGQGASVILEAV"
     gene            11244..13451
                     /locus_tag="PLB01_06305"
     CDS             11244..13451
                     /locus_tag="PLB01_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQQ76948.1"
                     /translation="MATLVEETVPVKNAVPAPSPFRLTVEADGVAVLEFDTPGEKVNK
                     YSRPVILELDRVLDGLAARNDVKALVLISRKPGVFIAGADVNEIAKADAGSDPEMIRG
                     PHRTFNKLANLPFPTVAAIDGACVGGGCETVLSMDWRLASDSPKTQIGLPEIKLGILP
                     AWGGTTRLPRMIGLAGALDVILAGKVLDAKRAKRAGLVDEVVPAPLLEEVAKAWARKK
                     IGTPKRSNAGAGGGPARVKAPTFMEKVLEGPAKSVVFNKARASVMKETKGHYPAPLAA
                     LSVVERGFGAPFDKALEYEVEGVGTLIGTPTMRALVGLFFRMEEVKKETGVEALGVGA
                     VKPRKIGRVGVLGAGVMGGGIAQLAADKGLPVRMKDIKPEALALGYAQAARIWKEKLK
                     KRRMTRAEFAGKMSLLGGSLDYAGFETCDITIEAVLEKMAVKHAVLAEWERVVPATAI
                     FASNTSTLPITEIAANAAHPERVVGMHFFNPVYKMPLVEVIYGQKTDPEVTATIFDLA
                     KRMGKTPVVVKDSPGFLVNRILGPYIAEGARLVLEGVDFTAIDKSMRAFGMPVGPIEL
                     LDDVGIDVAAKASETLSKTWPDRMPRDPALDKLVAAGRIGRKAKKGFYSYEGERRGGP
                     DPAVYAELGLSSPSKNLSSSEIQDRLVLPMINEAAYCLSEGVVASPAKLDLAMIFGTG
                     FPPFRGGLCAHADALGAKAVVESLQRLATEHGGRFAPAPLLIEMARTGKRFFE"
     gene            13518..15278
                     /locus_tag="PLB01_06310"
     CDS             13518..15278
                     /locus_tag="PLB01_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQQ76949.1"
                     /translation="MSTVKDETTTEVESFLKSLYQGHIAEDLVFPYPEIPADVKETVG
                     AFADAWTDFDAAHLNSEKMDAEHHFPREAVKAMGELGVMGMTIPEEYGGSGFGSAAYC
                     RMTETVAPLDASASIVIGAHLSIGTKPVVLFGSEEQKKKWLPDIASGKLVGAFCLTEP
                     EAGSDAGSLKTTAEYDAATDTYVLNGTKQWISNGGFATFFTVFARVPSGGADGHKEVS
                     CFAVVTNPDGTLPGLTRGPEEKKLGLCASSTCQIIFENCRIPAANLIGEKGHGFKYAL
                     ETLNTGRTSLGAGSVGGSKWMIKLAVEHATQRKQFKQRIADFEMIRHKITKMTATTYA
                     LESMVYLTASLVDRHIDYSLEGACCKVFGTEVLWQNINDGLQIAGGNGFMNEYPYGRA
                     LRDSRINMIFEGTNEILRLLIALSGMKELGDSLKEVQSALKSPLAQRGILTEYAHKRM
                     KGVITHDKLTKVHPSLAPEADQVGRYAGVFANACETILRKHGKKVIDREYQQERIADV
                     LIDLYAQIATLSRVTSSIAKKGEEKAAGEIEIARWFCNAAKHRMVANLKALEKNRDAD
                     CTKISDRVYESGGYPYDLWA"
     gene            15298..16233
                     /locus_tag="PLB01_06315"
     CDS             15298..16233
                     /locus_tag="PLB01_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ76950.1"
                     /translation="MTVPTPFPVGPVNVHLILRDPVTIVDSGPLTDAAWDVLVAGLAA
                     HGLAVADVKRILLTHGHHDHYGLASKVADVSGAQMWGGALDRNNFRQVRHPKLLLDDM
                     ARSGFGVATRFAVVAAIAAVDHFAKPLEAWDELRGGETLSGDGWSVLVRSTPGHTPGS
                     LTFEVPEAGVLFTGDTVLKDITPNAIIDEDPLDPGRPFHSVSTYFRTLEEIGRTNAGE
                     TLLTGHGAPIPDWHVHKGGLDRRYRLRVDQILRQLAKGPATVRDLVTAIFPRVKTLNV
                     FLAFSEIAGFLMYLEDEGRVERLVEETRDRYRLVA"
     gene            complement(16253..16942)
                     /locus_tag="PLB01_06320"
     CDS             complement(16253..16942)
                     /locus_tag="PLB01_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HQQ76951.1"
                     /translation="MKISRRSILACVLVLAAVASFAPAALAQGVTLFQNHYLSGPHQT
                     FYDDVPDLKYTQFGSRRASSIAVPPGCTAVLYQYPHYKGRSTVFRDDDNNLGNTAVGE
                     YAASSLRVNCRGHYPGWQPEPEPERRHGGWEGGWGHWESGQRGAILYRDREGKGPRAF
                     FDRDVPDLDRTRFGSRMASSIDVTPGCIVTLYELPGYRGRHTDFRERDNNLKNTAVGE
                     DTVSSLRVRCR"
     gene            complement(16965..17705)
                     /gene="thiD"
                     /locus_tag="PLB01_06325"
     CDS             complement(16965..17705)
                     /gene="thiD"
                     /locus_tag="PLB01_06325"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HQQ76952.1"
                     /translation="MAPATALSVHAADPTGTAGLQGDLAVFSALGVRCASVVTAIAAR
                     TGRAAGVLHEVPAAAVRAQLESLVEGPLPSAAKVGFLSKTPVVRLVAADLVRRHIPFV
                     VDPVLVSRTGPRLLGAPSWAAFLKELLPFAALVTPNLPEASLLAGFPIRGEGDAKRAA
                     RAIQAYGPRAVLIKGGHAEGDVVVDGLLDGRTWRAFPSPRLGEWAVPGAGDTLSAAVT
                     AHLARGETLPDAVEQALQYVRRAIAAGL"
     gene            17753..>18314
                     /gene="bcp"
                     /locus_tag="PLB01_06330"
     CDS             17753..>18314
                     /gene="bcp"
                     /locus_tag="PLB01_06330"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HQQ76953.1"
                     /translation="MSEENMVPAALTAGSIAPDFTLPARSGKTISLHDFAGQKDVLIY
                     FYPKDDTPGCTREACSLRDGWADLGKAGIEVLGISRDDAASHNAFASKYNLPFELLTD
                     ADHAVHEKYGAWGQNPNPAWGVGPLRKSFLVGKDGKVKHVFDKVDTERHADQVLRAAG
                     FAPAPKPAPAPAPAPAPAPKPAAPAPP"
BASE COUNT         2972 a         6231 c         6361 g         2750 t
ORIGIN      
        1 tcgaggccgc gcgtggggtg aatcgcgacc acgagccggg gcgatcgcgc gagcccgcgc
       61 gcgaggacga gcttctgctg gtttccgccc gagagggcgc ccgcgcgggt cgacggcgaa
      121 ggcgcgcgca ccgcgaacgc ctccatccgc tcgagcgcgt gcgtccgcat cgcggcgcgg
      181 tcgaggagga ggcgccccgg aggatcgggc agcgcgaggt tctcggcgag gtcgaggtcg
      241 aggacgagcc cctcgtcgcg ccggtccgcc gggacgaacg cgccgccggc cgcgcggaat
      301 gcgagcgggt cgccggacgg gggcagcgcg gccccgccga cgcggacctc tccggtgaag
      361 ggggtcagcc cggcgatcgc ggacgcgatc gaatcggctc cgttgccgtc gatggcgagg
      421 aggacgagca actcgccggg ggagagggaa agagaaagaa gttcttgctt cccttgtgag
      481 ccgcgcgggg caaagcgaga caagctgagg tttgacgagc cttcgcggag cccggcggcg
      541 ctccgcgccg cgttcttcga aggtcgggtc gcgccggtgc ggcgggcgtc gcctcgacgc
      601 ccctcgaccg gctctccgcc ctcttcacct tctaagaaga tcttcttctc tctttcttct
      661 tcgttctcgc tctcttcggt gccggagcct gcgatcagga ggccgccgat ctcttgcgcc
      721 gtcgtctcct cgattcggca cgtcttcacg gtctcccccc gcgcgaggag cgtcagcctg
      781 tcggcgcccg cgaagacctc ggcgaggcgg tgggtgatga ggacgatcgc ggcgccgccg
      841 tcggcctgct tccggagggc ggcgaagagg gccgcggtct cgtccgggga gaggacggcc
      901 gtcggttcgt cgaggatgag gacgtccggt tcgccggcga gcgcgcccgc gatctcgatg
      961 cgctggcgcg tgcccacggg cagcgtgtcc acgcggtcgc cggggtcccc gaggtcgagg
     1021 ccaaacgtct ccgcgagccg cgccacgcgc gcccggcgcg cccgcccgct ctcgacgagc
     1081 ggggcgccgc gatcgagcaa ggcgaggttg tcggcgacgc tcgcggctcc gacgaggagg
     1141 tcgtgttgcg gcacgagtgc gagacccgcg tcgcgcgcgt cccgcgcccc cgcgaaccgg
     1201 acggaccgcc cgccgcgcag gatctcgccg gaatcggccg cgaggcggcc cgcggcgatc
     1261 gacacgagcg tcgacttgcc ggccccgttc acggcgtgga tctcgccttg aaagacgacg
     1321 agatttgcct cggaaagagc ttgtgtttcg ccgaagcgct tggaaatcag ccgcagctcg
     1381 agcgcgggaa cggaggagga agacaaagac ggggagttct tagaaggttg aagaggcact
     1441 tcgctggcgg atccgccact gccgcaccca gacactccgt tcattcaccc ttctaagcaa
     1501 cggcgcgccg caggcgcgcg ccttccctgg tcagcggcgc ggagcgccgc cgcagaatct
     1561 ctcttcttca gaagttcccc cgagggacga ccagcgttcc cgcccggatc ttctcttccg
     1621 cggccttcag ctcgtcccgc acggccggcg gaatgttcgc agtcggcgac aggacgaacg
     1681 acacgacgcc gcttccgagg ccgtagcgca gcggcttgcc gtcgaacgtg ccggccttga
     1741 cgcgtcgggc cgtctcgacg aaagcgcggg gcatgtcgat caccgcggac gcgaggatgg
     1801 agggcgagag cgcgttctgg tccttgttcg tgccgaacgc cgcgatcttc cgctcctcgg
     1861 ccgccttgaa gacgccgagc gccgcggcgt cggcgttctg gaacagggaa tcggcgccct
     1921 tgtccgcgag cgcgagcgag gcggccttcg cgcccgcgac gtcctcgaac gagcccgtga
     1981 agaccgacgt cgccgtgccg gcaccgttcg cggccttgaa gcccccttcg aaggccagga
     2041 acgttgattt gatggacgga agctcgactc cgccgaccat ccccgccttc ccggtcctcg
     2101 tcgtccgtcc ggcgaggaga ccgcagaggt acgccgcctg ctcgagctcg aacacgatcg
     2161 gcgcgaggtt cggagccgtg cgcgagcccg acgtcgtcac gaagacggtc ttcgggtact
     2221 ccttcgcgac gcgctcggcg gcgtcctgga attcgaagcc gtggccgaag acgaggctcg
     2281 tccccttggc cgcgtaggcg cgaaactgct cttcgaactc ggagggagac ttgacctgca
     2341 cgtgggaaac gccggcgccg agctcccttt cgatggcctt gagtccttcg tacgcgccgg
     2401 cgttccatcc ggcgtcggag atcggccccg gcgtcaggag cgcgacggtg agcgccgggg
     2461 cgggagcctt cggcgtctct cttggccctc cgcaggcggc gagggcgagg gcggcggcgc
     2521 aggcggcaga cggacgcacg gaacgacggc tcacgggtcc tcctcgtcgg ctccttatga
     2581 tcatgccatg gcgctcgtgc tcgataccgc ctcgaaggcg cgcattctcg acttcgtccg
     2641 tccgctctcc gtggggctcg acgggatgac gaactacgga tttgtcgaga ggagaattcg
     2701 gatttcggat ttcttagaag gtgaagaggg gaggggcgaa gcgccggacg gcgcccgagc
     2761 ggatcccgac cggctgtttc ttctgtgcgc ttttgcgggg ctcccctccg cacgccgcat
     2821 caccccggat agccgcgagg acctgctcct gcgctccctc cgtcttccac tcgaagaaat
     2881 ccgaatcctg gtctcttctc tctctcgcct cgagtcctcg cccgagacgc cggaggagaa
     2941 gatcgtccac gacgcgatgc tgctcgagag cgtcggggca tacggcgtga cgcagatcct
     3001 ggtcgccggc gtgcgcgaga ggatgacgct ggcggagatg gcggacgaga tcgacgcgag
     3061 gatggacgcc gcgcgtttcg tgacggaggc cggccgccgg ctcgcggggg agcggatcgc
     3121 ctacgcgaag gacttcgccg cgaagctgcg ggccgaggcg gcggagttcg agacgccgag
     3181 ctgactcagt agaccgcgtc gatcccgacg tccagcctca cgttggagag gatcatgacg
     3241 tgtccggttt tcgccgctct cgccatgctt tcgtccgggc cggccatcag aaagccgaag
     3301 ttcatcgtgg gatcgtcgat ccagccctgg acgatcgcct tcatctggcc ggcatccgac
     3361 aggttgaccg cgttgccagg gtgattccac atgtccatcg aggtcccgtt ccagggctgc
     3421 gttatgaccc agagcggcct cgcgccgtcc ggagtgccgt cgttcttctc gtagctgagt
     3481 ttcgcccact tcaccttgcc cttcacgccc ttgagatcga cgaagatgtg gctccggtac
     3541 gtgacgctga cctgttttgg tttgttgtag tcgttgatga atccgacctt gacggtgcct
     3601 gcccccggct cgacgggccc ccacgtcgaa gattcctgtt ttccggtcga cgcgaaccag
     3661 tgggatccgt tcttcgtcgt ggccgggagg atcggattcg agaacttcac ggttctggcg
     3721 acgtgaaaga ggccgctgtc gcccttcgct tcgccgttca ccgtctcgat ccggacccgg
     3781 taatcaccct cggtcagagc cggcacgtcg ttcacgcccg ggagctgcca gctgtgcctg
     3841 cccgattgga gcggtcgctt ggtctcgatc gtgagtgaga tttctccggt gcttctcagc
     3901 agcaggatcc gggcgaactc cttcagcagg ttctccgact gctgccaggt gatgtcgtac
     3961 gtttgcgtgc gctgccacgt ggcgccggca gccgggctcg tcaccgtgat ggtcttcggg
     4021 gcgagggctc ccggggcgag cgcgacgttc gggcgggctg taggcatctc cgcgatgccg
     4081 aacggttgcg ggctctctcc gattaccgtc ggattcagcg cctcgatccg gatgcggaac
     4141 ccggtgccct tttccgtcag cgacgtgtag cccggcatca gcttgccgac cgtccaggcg
     4201 aacgagcccg tgccagccgg caccttggcg atcaggcccc ggccgaggtt ccagccctcc
     4261 tgctgaaggc tgatccggac ctcgcccgtg acaccggacg cggtccagac gatgttctgg
     4321 gtcgtgccga gaacccagct ctcgccgccc ttcggcgaga cgagagtgag ctttggagtc
     4381 gtgggggacg cgggctgcac ggccagaagg acgagacatg ccagggaggc aagggcttgc
     4441 atcgagcact cctccagagg cgaggggagg cctctcgatt caatctaggc cctcgcggcg
     4501 ggaaccgccc gtcggcgagc ccggccgccg gctaggcggc ggaccggccc gcgtcgaccg
     4561 cgacgacctg gccggtcatg gagcggttct cgaggatcgc gaccgccatc gaggtcaggt
     4621 cctcgacggc gacggggcgc ccgaggggag tcttcgcgac gagcgcgtcc acggcctcgc
     4681 ggctcatgtc ggtcggcggc atcacgacgc cgggcgcaat cgcgttgacg cgcacgttcg
     4741 ggccgagcgc gacggcgagg ttgcggacga gggcgttgat cgcggccttg gcggccgcgt
     4801 gcggaacgtg cgtcggccaa gcctgcgtcg cggcgacgtc ggacacgaac aggatcgaac
     4861 ccgacgagcg ggccagctcg ggcgcggccg cctgggcgag gaagaacgcg gcgcgcggtc
     4921 cggcgcggaa tacggcctcc cagtcgtcga gcgtgacggc tgcgaccggc ttcgggatcc
     4981 acggcgacgc ggcgtggacg aggacgtcga gccgcggggg cgaagaggga ggggaaaaga
     5041 ggggagaaga gaagagttct tcgaatagtt tgatcggcgc gccgtcgacc gcgagatcga
     5101 tccggaacgc ctcggcccgg actccctctt cctttctaag gaaatcgacg gcgcgacccg
     5161 cttcttcggg atcgcttctg taggagaggg cgatcgagta gccgttcttt gcgagggcct
     5221 tcgcgaactc gaacccgagc cgtttcgctc cgccggtcac gagggcgacc ttcatgcgcc
     5281 gtctcctccg gccgcgggcg gcacgagcgt cgcggcctcg tccatgagct tgttcgtttc
     5341 cgccatggcg acgacctccc gcacgagcgc ctgagcatgc aggagcacgg tcaggatcgc
     5401 ggccgcgagg tggagccacg ccggggttgc gcccgtgtgg gccccggccc cgaggagccc
     5461 ggccgcgaga gccgagagcg gggcgaaagt cgcgagggcg gacgttcggg ctttgaagtc
     5521 cttgacccgc ctcaggatgt agtcgcggcc ttccgcgtcg ggccgctttg cggcggcctc
     5581 cttgaggagc ttgccggtcc cgatgaagaa gaacaggacc atcgactgcg tgaacaacga
     5641 gaacagaacc gccgggacgg cgaccacgat gtggcgggcc gcggagaggc cgccgagggc
     5701 gtgagaaagc gtccagccgg accaggccga gaggcccagc gcggccagag tgaacaggga
     5761 gagaagagtc agggccagcg ccacgggaac gtcactataa tccgggttcc cggctcaaga
     5821 tttggaggag atgcgtcatg acaccacgca cgctggtcga catcttcagg aacctcgaga
     5881 agaacccgaa gcccgatctc ctcctcgtca agaagtccgg cgcgtggacg cccatctcga
     5941 ccgcgcagtt catggacagc gtcaaggcgg tgtcctgtgc gttcgaaggg ctcggggtga
     6001 agccgggcgg ccgcgtcgcg ctcctgtccg aaaaccggcc cgagtgggcg atcgtggact
     6061 tcgcctgcca gtgttacggc gcggtcctcg tgccgatctt cccgacgatg gtctcggatc
     6121 aggtcacgta cctcctgaag gattccggcg cgacggtggc gttcgcgtcg aacgaggatc
     6181 aggcccgaaa ggttctcgcg gcgcgggccg catcggccga ggtcaacgcg tcgctccagc
     6241 acgtcttcag cctcgacacg tcgacgctcg ggggcgtcga gccgttcacc gacgtcctcg
     6301 agaagggcaa ggccgcccat gcggccgacc ccgccgcgtt cgagaagcgg gccgacgcgc
     6361 gcacgccgga cgacctcgcg acgttcatct acacctccgg cacgacgggc gagccgaagg
     6421 gcgcgatgct cacgcagcag aacttcgttt cgaacgtcct tgcgggctgc tcgatcctgc
     6481 cgctcgattc ctcgtgtgtc gcgctctcgt tcctcccgct cgcgcacgtc ttcgagcgga
     6541 tgctcgagta cgtctactac catcgcgcgg cgacgatcgc gtacgcggag tcgatcgaca
     6601 aactgcgcga caacatgggc gaggtgcacc cgcatctctt cggcgcggtt ccgcgcgtct
     6661 acgagaagat ctacgcgcgc gtccaggaga acctcgccaa ggcgccggcg ggcaagcaga
     6721 agctcttcgc gcaggccatc gacgtgggca agaaggtcgt cgcgctcaag gcgaagaagc
     6781 agacgcccga cttcgcgctc gcgctccagc acttcgtctt cgagcgcctc gtgtatcgca
     6841 aggtgcgcgc ggccctcgga acgcggttcc gctacgcggt ctccggcggc gcgccgctcg
     6901 cgaaggagct cgcggagttc ttctgggcga tcggcgtgga gatctacgag ggctacggcc
     6961 tcacggagac gagcccggtc atcgccgtga actgcccgtc ggcctggcgc ctcggcgcgg
     7021 tcggaaagat cctgcccggg gtcgaatgcc ggatcgcgcc ggacggcgag atcctcacgc
     7081 gcgggccgca cgtcatgaag ggctactaca acaagccgaa ggagaccgcc gaggcgatcg
     7141 actccgaggg ctggttccac acgggagaca tcggcgtcat cgacgcggac ggcttcctcg
     7201 cgatcacgga ccgcaagaag gagatcctcg tcaactcgaa cggcaagaac atcgccccgg
     7261 cgccgatcga gagcttcctc aagagccagg acttcatctc gctgcccgtg atcatcggcg
     7321 acaagaggaa gttcctgtcc tgcctcatca tcccgaactt cgagaagctc gccgcgtggg
     7381 cggaagcgaa cggcttcgcc gggcgcccga tggagcagct cgtgaaggag ccgcgcgtcc
     7441 tcgcgctgtt ccagacggcg atcgaccgct ggaacgaagg caaggcccac gagcagctcg
     7501 tccaccgctt cgcgctcctc ccgaaggacc tcacgatcga ggacggggag ctcacgccga
     7561 cgctcaaggt caagcggcgc atcgtcgacc agcactacaa gcacctgatc gacgagatgt
     7621 acgagggctc gggcgcctag gccgccgtcc ggtcaaggga tgcgggtcgt cgtcgctctc
     7681 ggagggaatg ccctgcttcg gcgcggggag cccatgaccg cggagaacca gcgcgcgaac
     7741 gtgcgccggg ccgcggaggc gctcgcgccg gtggcgcgcg agcacgagct cgtcatctcc
     7801 cacggcaacg gcccgcaggt gggcctcctt gcgctgcagg ccgagtcggg cctcgacgcc
     7861 gcgcacttcc cgctggacgt cctcggcgcg cagaccgagg ggatgatcgg ctacatgatc
     7921 gagcaggagc tcggcaacct tctcccgttc gagaagccgt tcgcgacgct cctcacgatg
     7981 gtcgaggtcg acccggccga cccggcgttc cggaacccga cgaagcccat cggccccgtc
     8041 tattccgagg aggacgcgag gcgcctcgcg aaggagcgcg gatgggcggt cgcgcccgac
     8101 ggcggaaagt ggcggcgcgt cgtcgcctcg ccgcagccca agcggctctt cgagatccgg
     8161 cccgtgaagt ggctcctcga gaaggggacg atcgtgatct gcgggggtgg cggcgggatc
     8221 ccgacggtgt acggcgagga cggaaagctc catggcgtgg agtgcgtcat cgacaaggac
     8281 cgcgtcgcgt ccctcctcgc gcagcagctc gacgcggact tcttcgtgat ggcgacggac
     8341 gttccggccg tgtcggtcgg ctggggaacg ccgtcggcga aagcgatccg ccgggcgtcc
     8401 ccggccgcga tcgggcgctt cgacttcgcg tcgggctcga tgggtccgaa ggtcgaggcg
     8461 gcccgcgact tcgccgagcg gaccggcaag acggccgcga tcggcgcgct cgaggacatc
     8521 ccgcggatcc tcgcgggcga ggcgggaacg ctcgtctcca acggcgtcgc gacgatcgag
     8581 tgggcggcgg ccgagagcct ctccctgttc acggagtaga aggcagggcc gccggcggcg
     8641 gggcgggctc cagcttgacg gcgatgagcg gcgggtcgag gcggtggatc gacccgctcg
     8701 cggcgtccac ggcgaacatg gcgccgggca ccacgagcgc tccgatcgcg aggcccgtcg
     8761 cgccgctgct gatctcgtcg agtccctcgc tgttctgggc gccgatcgcg gcgccggcga
     8821 ccagccccgc cgggagaccc acgaagttga tccacgtgtt cggggacgcg ccgcgctcga
     8881 gggggacctt ccgggtgacg tagccctctt tctcgatgac gacgacgagg cccttcgcgg
     8941 cgcgtttcag gacgaggtcg accgggctcg tatgagtctc gcccgcgacc ctgacggtcg
     9001 cgccgggagg atcggtttcc acgtggatcg tctggttcgg cccgttcagg atcgtcgcgc
     9061 agccgggaag aaacggggag aaggcgagaa cgaacgcgaa ggggagcgcg cgtcgggtcc
     9121 gcatgccggg cctcctttgg ctcgaatctg gagccggccg gcgcgtggat cttcggggat
     9181 ttccagcgaa ttcccatgac ttcagcggcc cgccggacgt ccaagatcgg cccttgactt
     9241 cctggcgcag cgcgtcatag cctactgaat gatggctcat tcatcgggcc gggaaggcct
     9301 cggtacggat gcggcggccc ttgcggcggc ccccgaaaag gcattcccgg gcgccgatcc
     9361 cgagaagcgc cgccggatcc tcgaggccgc gatccgcacc ttcggacgac ggggcttcca
     9421 cgaggcccgc atcgcggaca tcgcggccga cgcgaaggtc gccgagggca cggtctacct
     9481 ctatttccgc aacaaggagg acctgctcgg ggtcgtgttc gacgagtcga tggacgacgt
     9541 cctcgagaag ggccgggccg tcgcgcgatc ggacgctccc gcagccgagc gcctcacggc
     9601 cctcctcgac ctccacttcc agttcatcgg ctcggaccgc gacctcgcgt ccgtcttcca
     9661 gatcgagctt cgccggagcg cgcggctcgt cgagcgcttc tcgcgcagca agctcgtcga
     9721 gcacttccgt ctcctcggcg acgtcctgcg cgacggcatc gcgcgcggcg agttccgcag
     9781 ggacctcgac ccgcggctcg ccgtccgcat tctcttcggc gcggcggacg agatcctctc
     9841 ggagtggctg ctgtcggggg agaagaagcc gagcgccgac gcgaagcagc tcgtggggac
     9901 gctgctcagt ggctttgcgc agacgagaga gagagaaaag aagaggattt ctttgaaggt
     9961 gaagaaggcg aacaggagta accccaagtg agcaaaaccg cattcatcgt tggcggcgcc
    10021 cggacgccgt tcgcgcgcat gggcacggac tacacgcgcg tgtcggcggt cgacctcggc
    10081 cgcgccgcgg ccgtcgaggc gatggcccgg tccggcatcg agccctcggc cgtcgaccag
    10141 tcgatcttcg gaaacattgc gacgccggtg gacgccgcga acatcgcgcg gatcatcgcg
    10201 ctccgcgccg gcgtcccgaa ggagaggccg gcgcactccg tctcgcgcaa ctgcgcgtcg
    10261 gggatcgagt ccgtcgtcga ggcggcgcgc ctcgtcgcga tcggcgaggc cgacgtcgtc
    10321 ctcgcgggcg gcgtcgagaa catgacgcag atcccgttcc tctaccgcga cagcgtcaag
    10381 ggcgtgttcg cgaaggcggg gatggcgaag accgccggcc agcgcctcgg cgcgctctcc
    10441 ggcatgccgt ggaaggatct cctgaatccg gtcatcggac tcaaggaagg gctcaacgac
    10501 cccgtctgcg acctcgggat gggcgacacg gccgagatcc tcgcgaagga aggccgcatc
    10561 acgcgggaag agcaggacca cttcgcgctg cgctcgcacc agcgggcgac cgaggcgcgg
    10621 gcgaagctgg ccctcgagat cgtccccgtc ccggtcccgc cggactacga ccgcgtgctc
    10681 acgttcgaca acggcgtgcg cgagaaccag acgatggagg cgctcgcgaa gctcaagccg
    10741 gtcttcgacc gcaagtacgg caccgtgacg ccgggcaacg cgtcgcagat cacggacggc
    10801 ggcgcggccg tcgtcgtggc ctccgaggat ttcgtcgtgc ggcacggcct gaagcccctc
    10861 ggccgcctga agagctgggg cttcgcgggc ctcgagcccg agcgcatggg cctcggcccc
    10921 gcgcgctcga cgccgctcgc cctgaagaag gccgggctct cgatgaagga catcggtctc
    10981 gtcgagatga acgaggcgtt cgcggcgcag gtgctcgcga acctcaggga gttcgccgcc
    11041 tcgaaggagc tcggcgagat tcccatggac atcctgaacg tgaacggcgg ggccatcgcc
    11101 ctcggacacc ccgtgggctg cacgggcacc cgcctcatcc tgacgctcct cctcgagatg
    11161 cagcggcgcg aggtccggca cgggctggcg accctctgcg tcggcggcgg gcagggcgcg
    11221 tcggtgatcc tggaggccgt gtgatggcga ccctcgtcga agaaaccgtt cccgtgaaga
    11281 acgccgtgcc cgcgccgtct cccttccggc tcaccgtcga ggccgacggc gtcgccgtgc
    11341 tcgagttcga cacgccggga gaaaaggtca acaagtactc ccgtcccgtc atcctcgagc
    11401 tcgaccgcgt gctcgacggc ctcgcggcgc ggaacgacgt caaggctctc gtcctcatct
    11461 cgcgcaagcc cggcgtcttc atcgcgggcg ccgacgtgaa cgagatcgcg aaggccgacg
    11521 cgggctccga ccccgagatg atccggggcc cgcaccgcac gttcaacaag ctcgcgaacc
    11581 tgccgttccc gacggtcgcc gcgatcgacg gcgcgtgtgt gggcgggggc tgcgagaccg
    11641 tcctctcgat ggactggcgc ctcgcgtccg actcgccgaa gacgcagatc ggactgccgg
    11701 agatcaagct cgggattctt cccgcatggg gcggcacgac gcgcctcccg cggatgatcg
    11761 gcctcgcggg ggcgctggac gtcatcctcg cgggcaaggt cctcgacgcc aagcgcgcga
    11821 agagggccgg cctcgtggac gaggtcgtcc ccgcgccgct cctcgaggaa gtcgcgaagg
    11881 cctgggcgcg gaagaagatc gggacgccga agcgctcgaa cgcgggagcc ggcggcgggc
    11941 cggcgcgcgt caaggcgccg acgttcatgg agaaggttct cgaaggtccg gcgaagagcg
    12001 tcgtcttcaa caaggcgcgc gcgtcggtca tgaaggagac gaagggccac tacccggccc
    12061 cgctcgcggc gctctccgtg gtcgagagag gcttcggagc gccgttcgac aaggccctgg
    12121 aatacgaggt cgagggcgtc gggaccctca tcggcacgcc gacgatgcgt gccctcgtcg
    12181 gcctcttctt ccggatggaa gaggtcaaga aggagaccgg cgtcgaggcg ctcggcgtcg
    12241 gcgccgtgaa accgcggaag atcgggcgtg tcggcgtcct cggcgcgggc gtcatgggcg
    12301 gcggcatcgc gcagctcgcc gcggacaagg gcctgccggt ccgcatgaag gacatcaagc
    12361 ccgaggcgct cgcgctcggc tacgcgcagg ccgcgcggat ctggaaggaa aagctgaaga
    12421 aacgccgcat gacccgcgcg gagttcgcgg ggaagatgtc gctcctcggc ggatcgctcg
    12481 actacgccgg cttcgaaaca tgcgacatta cgatcgaggc cgtgctcgag aagatggccg
    12541 tcaagcacgc cgtcctcgcc gaatgggaga gggtcgtccc ggccaccgcg atcttcgcgt
    12601 cgaacacctc gacgctcccg atcacggaga tcgccgcgaa cgcggcccac cccgagcgcg
    12661 tcgtcggcat gcatttcttc aacccggtct acaagatgcc gctcgtcgag gtcatctacg
    12721 gccagaagac cgatcccgag gtcacggcga cgatcttcga tctcgcgaag cgaatgggga
    12781 agacgccggt cgtcgtgaag gactcgccgg gcttcctcgt gaaccgcatc ctcgggccgt
    12841 acatcgccga gggcgcgcgc ctcgtcctcg agggcgtcga cttcacggcg atcgacaagt
    12901 ccatgcgcgc gttcgggatg cccgtcggcc cgatcgagct cctcgacgac gtcgggatcg
    12961 acgtcgccgc gaaggcctcc gagacgctct cgaagacgtg gccggaccgc atgcctcggg
    13021 acccggcgct cgacaagctc gtcgccgcgg gaaggatcgg acgaaaagcg aagaagggat
    13081 tctattccta cgaaggggag agacgcggcg gaccggaccc ggcggtgtat gccgagctcg
    13141 ggctctcttc accttcgaag aatctttctt cttccgagat ccaggaccgt ctcgtcctgc
    13201 cgatgatcaa cgaggcggcg tactgcctct ccgaaggcgt cgtcgcgagc ccggcaaagc
    13261 tggacctcgc gatgatcttc ggcacgggct tcccgccgtt ccgcgggggt ctctgcgccc
    13321 acgcggacgc gctcggcgcc aaggcggtcg tcgagtcgct ccagagactg gcgacggaac
    13381 acggcggccg tttcgcgccc gccccgctgc tcatcgaaat ggccaggaca ggaaagagat
    13441 tcttcgagtg agaaagagcg gccggtcgct tcggcccgca ttcaccttct aggaaaaaca
    13501 gcacctcagg agccgacatg tccacagtca aagacgagac caccaccgaa gtcgaatcgt
    13561 tcctcaaatc gctctaccag ggccacatcg ccgaggacct cgtcttcccg tatccggaga
    13621 ttccggccga cgtgaaggag acggtcgggg cgttcgcgga cgcctggacc gacttcgacg
    13681 ccgcgcacct gaactccgag aagatggacg cggagcacca cttcccgcgc gaggccgtga
    13741 aggccatggg cgagctcggc gtcatgggca tgacgatccc cgaggagtac ggcggcagcg
    13801 gcttcggctc cgccgcgtac tgccggatga cggagacggt cgcgccgctc gacgcgtcgg
    13861 cctccatcgt gatcggcgcc cacctctcga tcggcacgaa gcccgtcgtc ctgttcggaa
    13921 gcgaggagca gaagaagaag tggctcccgg acatcgcgtc cgggaagctc gtcggcgcgt
    13981 tctgcctcac ggagcccgag gccggctcgg acgcgggctc gctcaagacg accgccgagt
    14041 acgacgcagc cacggacacc tacgttctga acgggacgaa gcagtggatc tcgaacggcg
    14101 ggttcgcgac gttcttcacc gtgttcgcgc gcgttccctc gggcggcgcg gacggccaca
    14161 aggaagtctc ctgcttcgcg gtcgtcacga acccggatgg aacgctcccg ggcctcacac
    14221 gcgggcccga ggagaagaag ctgggcctgt gcgcctcgtc gacgtgccag atcatcttcg
    14281 agaactgccg gatccccgct gcgaacctca tcggcgagaa ggggcacggc ttcaagtacg
    14341 ccctcgagac gctgaacacg ggccgcacgt ccctcggggc aggctcggtc ggcggctcga
    14401 agtggatgat caagctcgcc gtcgagcacg cgacgcagcg caagcagttc aagcagcgca
    14461 tcgccgactt cgaaatgatc cgccacaaga tcacgaagat gacggcgacg acgtacgccc
    14521 tcgagtccat ggtctacctg accgcgagcc tcgtcgaccg gcacatcgac tattcgctgg
    14581 aaggcgcgtg ctgcaaggtg ttcgggaccg aagtcctctg gcagaacatc aacgacggcc
    14641 tccagatcgc gggcggcaac ggcttcatga acgagtatcc gtacgggcgc gcgctccgcg
    14701 acagccgcat caacatgatc ttcgagggga cgaacgagat cctccgcctc ctgatcgcgc
    14761 tctcgggcat gaaggagctc ggcgactcgc tcaaggaggt ccagagcgcc ctcaagagcc
    14821 cgctcgccca gcgcggcatc ctgaccgagt acgcgcacaa gcgcatgaag ggcgtcatca
    14881 cgcacgacaa gctcaccaag gtccacccgt cgctcgcgcc ggaggccgac caggtcggcc
    14941 ggtatgcggg cgtcttcgcg aacgcgtgcg agacgatcct ccgcaagcac ggcaagaagg
    15001 tcatcgaccg cgagtaccag caggagcgca tcgcggacgt cctcatcgac ctctacgcgc
    15061 agatcgcgac gctctcgcgc gtgacctcct ccatcgcgaa gaagggcgag gagaaggcgg
    15121 ccggagagat cgagatcgcc cgctggttct gcaacgccgc caagcaccgc atggtcgcga
    15181 acctcaaggc gctcgagaag aaccgcgacg ccgactgcac gaagatcagc gaccgcgtct
    15241 acgagagtgg cgggtatccc tacgacctct gggcgtgatc acggcttcat ccggacgttg
    15301 accgtcccga ccccgtttcc ggtcgggccg gtcaacgtcc acctgatcct ccgcgatccc
    15361 gtcacgatcg tcgactccgg cccgctcacc gacgccgcgt gggacgtcct cgtggcgggg
    15421 ctcgcggcgc acggcctcgc ggtcgccgac gtgaagcgca tcctcctcac gcacgggcac
    15481 cacgaccact acggcctcgc atcgaaggtc gcggacgtct cgggcgcgca gatgtggggc
    15541 ggcgcgctgg accggaacaa cttccgccag gtgcggcacc cgaagctcct cctcgacgac
    15601 atggcgcgct ccggcttcgg cgtcgcgacg cgattcgcgg tggtcgccgc gatcgccgcc
    15661 gtggaccact tcgcaaagcc cctcgaggcg tgggacgagc tccggggcgg cgagacgctg
    15721 tccggtgacg gctggagcgt tctcgtgcgt tcgacgccgg gccacacgcc cgggagcctg
    15781 acgttcgagg tccccgaggc gggcgtcctc ttcaccggcg acacggtcct caaggacatc
    15841 acgccgaacg cgatcatcga cgaggatccg ctcgatcccg gccggccgtt ccacagcgtc
    15901 tcgacctact tccggacgct cgaggagatc ggccggacga acgcggggga gacgctcctg
    15961 accggccacg gcgcgccgat cccggactgg cacgtccaca agggcgggct cgaccggcgc
    16021 taccgcctgc gcgtcgacca gatcctgagg cagctcgcga agggtccggc gacggtgcgc
    16081 gacctcgtca cggcgatctt cccgcgcgtg aagacgctca acgtcttcct cgcgttcagc
    16141 gagatcgccg gcttcctgat gtacctcgag gacgagggcc gcgtcgagag gctcgtcgag
    16201 gagacgcggg accgctaccg gctcgtcgcc tgacggcggt ccttcgcggg gctcaccgac
    16261 accggaccct gagcgacgaa acggtgtcct cgccgaccgc cgtgttcttc aggttgttgt
    16321 ccctctcgcg gaaatccgtg tgcctgccgc ggtatccggg cagctcgtag agcgtcacga
    16381 tgcagccggg cgtcacgtcg atcgagctgg ccatccgcga gccgaagcgc gtccggtcga
    16441 ggtccgggac gtcgcggtcg aagaacgcgc ggggtccctt gccctcgcga tcgcgataca
    16501 ggatcgcgcc gcgctggccc gactcccaat ggccccagcc gccttcccat ccgccgtgcc
    16561 ggcgttccgg ctcgggctcc ggctgccatc ccgggtagtg gccgcggcag ttcacgcgga
    16621 gagacgatgc ggcgtactcg ccgacggcgg tgttcccgag gttgttgtcg tcgtccctga
    16681 agaccgtcga gcggcccttg taatgcggat actggtacag gaccgccgtg cagcccggag
    16741 gaaccgcgat cgaactcgcg cggcggctgc cgaactgggt gtacttgagg tcgggcacgt
    16801 cgtcgtagaa cgtctgatgc ggcccgctca ggtagtgatt ctggaagagc gtgactccct
    16861 gcgcgagggc ggcaggagcg aacgaggcaa ccgccgcgag gacgaggacg cacgcgagga
    16921 tcgatcgtcg ggaaatcttc atggaatcct ccgcgggcgg gactttacaa cccggctgcg
    16981 atcgcccttc gcacgtattg gagggcctgc tccacggcgt ccggcagcgt ctcgccccgg
    17041 gcgaggtgcg cggtgacggc cgccgagagc gtgtcccccg cgcccggcac cgcccactcc
    17101 ccgaggcgcg gcgaggggaa ggcccgccac gtccggccgt ccagcagccc atcgacgacg
    17161 acgtcgcctt ccgcgtggcc gcccttgatc aggacggcgc gagggccgta cgcctggatg
    17221 gcccgcgcgg cgcgcttggc gtcgccttcg ccccggatcg gaaagccggc gagcagggac
    17281 gcttccggga ggttcggcgt gacgagcgcc gcgaagggga ggagctcttt caggaacgcc
    17341 gcccaggaag gggccccgag gagccgaggg ccggtccgcg agacgaggac ggggtccacg
    17401 acgaacggga tgtgccgccg gacgaggtcg gcggcgacga gccgaacgac gggggttttc
    17461 gacagaaaac cgaccttcgc cgccgagggg agcgggccct cgaccaggga ctcgagctgc
    17521 gcccggaccg ccgcggccgg cacctcgtgg aggacgcccg cggcccggcc cgtccgggcg
    17581 gcgatcgcgg taacgacgga ggcgcaccgg accccgagcg cggagaaaac cgcgagatcg
    17641 ccctggagtc cggccgtccc cgtcgggtcg gccgcgtgca cggagagagc ggtcgcgggc
    17701 gccacgggcc aagcttactt tgctattctc cggcgccgga ggaatcgcct tcatgtctga
    17761 agaaaacatg gttcccgcgg ccctcaccgc cgggtcgatc gcccccgatt tcacgcttcc
    17821 ggcgcggtcc ggaaagacga tctccctgca cgatttcgcc ggtcagaagg acgtcctgat
    17881 ctacttctac ccgaaggacg acacgccggg ctgcacgcgg gaggcctgca gcctgcgcga
    17941 cggctgggcc gacctcggca aggccggcat cgaggtgctc ggcatctcgc gtgacgacgc
    18001 cgcctcgcac aatgcgttcg cttccaagta caaccttccg ttcgagctcc tgaccgacgc
    18061 ggaccacgcg gtccacgaga aatacggcgc ctggggccag aacccgaacc ccgcctgggg
    18121 cgtcgggcct ctccggaagt cgttcctcgt gggaaaggac ggcaaggtca agcacgtgtt
    18181 cgacaaggtc gacacggagc gccacgccga ccaggtcctc cgggccgcgg gcttcgcgcc
    18241 cgcccccaag cctgctcctg ctccggcacc ggccccggcc ccggctccga agccggccgc
    18301 tcccgcgccg cctc
//
LOCUS       DAOMFY010000051        18163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_965_length_18163_cov_6.966976, whole genome shotgun sequence.
ACCESSION   DAOMFY010000051 DAOMFY010000000
VERSION     DAOMFY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18163
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_18163_cov_6.966976"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1102)
                     /locus_tag="PLB01_06335"
     CDS             complement(71..1102)
                     /locus_tag="PLB01_06335"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQQ76954.1"
                     /translation="MNAARISGEGHAFHGATFGALFPGQLSEKPGMGEAFARRYDFVS
                     SLFEEISSRSGVRLAETFFGEGLPSLHDDKPAQVGVFAVSLAALEVLRREHGLVPDAC
                     CGYSLGTYAAFVAAGCLSREAALDALLEAERLLAEERAGGEADGTMGFVIGLGRADVE
                     AALAGVCPDLSRLAIATENAAAQFVLTGERDLVEEALDLMRPRALKAEMLTIGFPMHS
                     RSLESVSEKLKIFLEGGVRVGQPQSPLFAPMLGRRVGSSAEAAHVLSEQISLPSFFSP
                     TLLAMRAAGIHRFAEVGPGDVLTKLVRWTLRDAIVARPSLERPEDVHVFAATVLPAGP
                     RAVPAPVIP"
     gene            complement(1099..2022)
                     /locus_tag="PLB01_06340"
     CDS             complement(1099..2022)
                     /locus_tag="PLB01_06340"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQQ76955.1"
                     /translation="MPAPILTVDVEEWFHLCGHAAYSDPATWAAREKRVHVGTDRILE
                     LLAGTASKATFFVLGWVARQSPALVKRIAEAGHEIGCHGDTHRRVFELPPAEFREDVR
                     RSRDTLQAATGKPVSAFRAPEWSMLKADNPALAILVEEGFAIDSSLTTAPPVGDLANP
                     VRPAVLATSAGPILEVPPLMGTFFGRRALWGGGVCSRLTRERRVEGAIERSLAAGIPP
                     VLYAHPWEFDPEHPEMPGLSTVQRLVHFAGRGRTEARWRRWLKRWTFAPISSAASEEH
                     PGISANGKRGEGSGGPSAVSPPLAARSGAAA"
     gene            complement(2030..2725)
                     /locus_tag="PLB01_06345"
     CDS             complement(2030..2725)
                     /locus_tag="PLB01_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ76956.1"
                     /translation="MSEKNYEGFRPAALVPAFRCASTVGGVVTGLKRVVPEVLVVDDG
                     SEDGTGDAARAAGVRVLVRPANGGKGSALRDGLAVLLAKPVTHIAFVDADGQHDPADL
                     PALLDAAKAGADFVIGSRLKDAAAMPAKNYWANTIGDKVLGRMTGLPVEDGQSGYRVI
                     AADLLRPLRLRAERYSIENEILIKAAPRVRRFAAVPVKTIYGARSHYRPFRDTWITSW
                     LSVYYKTLDSDAS"
     gene            2781..3143
                     /locus_tag="PLB01_06350"
     CDS             2781..3143
                     /locus_tag="PLB01_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQQ76957.1"
                     /translation="MGSPLAFLPILMMLVGAMGVGALLLGLNYLLGRKVKTKRKLSTY
                     ESGMPLLDDSHKRISVKFFIVAMIFILFDIEAAFLYPWAVVFREGGMAVFVEMLVFVV
                     VLFLGFGYLWKKGAFDWN"
     gene            3145..3702
                     /gene="nuoE"
                     /locus_tag="PLB01_06355"
     CDS             3145..3702
                     /gene="nuoE"
                     /locus_tag="PLB01_06355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQQ76958.1"
                     /translation="MSGGANVPVAEQAGLPRTGEHGALSCGKALTFSKEARAELDRLL
                     TRYPTKQAALLPALWLAQKEWGWISREAVDHVASVLDLSPAFVWGVVTFYTMYNRAPV
                     GKNLIQVCTSISCHLNGAEEVFHECKKRLGDLKTGETSKDGKYTVIEVECLAQCDKAP
                     AVMVNDDDYAVITKDKLDAFLKGLG"
     gene            3707..5083
                     /gene="nuoF"
                     /locus_tag="PLB01_06360"
     CDS             3707..5083
                     /gene="nuoF"
                     /locus_tag="PLB01_06360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQQ76959.1"
                     /translation="MSGLETIPPVLLARLGKPDSRAIDGYVADGGYTSWKKVLAGAKT
                     GEWTPAKLTDMVKASGLRGRGGAGFPCGLKWTFVPAKDKRGEKPVYLLCNADESEPGT
                     FKDRLLIEQDPHQILEGMMLASYALDVKHAYIYIRGEMVHGAEYLNAAVAEAHAKGFL
                     GTDILGSGIDLTITVHRGAGAYICGEETALIESLEGKRGHPRIKPPFPAVSGVWGAPT
                     VVNNVETLCCVKHIVDRGADWFKGVGRNEKNTGPKLYAVSGHVEKPGVYEAPIGIPWE
                     QLLAMAGGVRGGRKLKAVIPGGSSAPILTAAEIAGIPMDFDGIAAAKSMFGSAAIIVM
                     DETTDIPKAVTNIAKFYAHESCGQCTPCREGTPWLLKMLRRIVNGEGKKTDLDLLLQI
                     SNQMGNGMTICVFADAAIAPVISGIVKFRGEFEAYIEKSQKPTFEQGASDDLTNAIAD
                     RTAAGIHS"
     gene            5162..6877
                     /locus_tag="PLB01_06365"
     CDS             5162..6877
                     /locus_tag="PLB01_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQQ76960.1"
                     /translation="MPKVTVDGRTVEVPADANALEACRAAGVDVPHFCYHPALSIVGQ
                     CRMCMVEVEGVPKIQASCTVPVRDGMVIKANTEKALAARNATMEFLLINHPLDCPICD
                     QAGECKLQDNAVAGGLPVSRTSEPRRQFPGYDRTDIGPHVIADMTRCIQCTRCIRFCQ
                     EITGTGELTFIERGGHTIVWTHEGKALDNDLSGCAADVCPVGALTTKEFRFRKRVWWL
                     DKTRSICDGCEIGCSLSIEHRDGVVYRYTPRVQPAVNDYWLCDYGRFRAEDLNARDFT
                     KPEVRVAGANTRKQTNWGEALDAVKAAIEKAKAEEKSSSKVMVIGSAHLTTEEAFLLA
                     TLCKDSLKVNAPEFTVDIGPKRRIRNKKDGWLYGTEAAPNSRGCEAAGLTRAKEAGTS
                     PLSLLLSKKSSSSPSVLYITDAAFTEWANDPAFVAELRKASVLIVHARKRNALTDVAD
                     IVLPVASLAGTEGTFVNAKGRVQRVDLAILAPPIVRTNLEIFLHLGAKYGAFDTQWTA
                     RDVFDRMTASVAGYAGLDWDSDDLVGNPPSVSPAAAYDALGLNKDADVEVFTRPSVAA
                     PDRKS"
     gene            6874..7923
                     /gene="nuoH"
                     /locus_tag="PLB01_06370"
     CDS             6874..7923
                     /gene="nuoH"
                     /locus_tag="PLB01_06370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQQ76961.1"
                     /translation="MSPTLSAALEGAGKIAFIWGVVLAAMLPNLIWMERRIAGLIQDR
                     PGPNRVGPFGLFQALADVLKMFMKEDIVPLYADRLLHTLAPFILLVPAMATFAVIPFG
                     SKIPLFGREIPLVVADVNIGILYVFALASVGVYGLVLAGWASNNKFALMGGIRSSAQV
                     ISYELSMGLAAVATLMAAGSLRLTRVVEYQAGGTFLGFLPLWNCFTQPLGCIIFIIAA
                     FAETNRIPFDLPEAEAELVAGYHTEYSGFRFGAFFMAEYLNLVASSMMMATLYFGGWS
                     LPFLPHSGLAGGLLSVAVFFAKTMCFVFLFMWVRWTLPRFRFDQLMDIGWKALLPLSL
                     LNLLWTGALVLLKVI"
     gene            7923..8441
                     /locus_tag="PLB01_06375"
     CDS             7923..8441
                     /locus_tag="PLB01_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQQ76962.1"
                     /translation="MPALSGELVVFTIASVLAIGFALAMVVQKTPVRSVLSLVVTFFG
                     LAIFSIQLAAPFIAVLQVFVYAGAILVLFLFVIMLLNLSQEATEEDSRPVQRWLGLVA
                     ALALGGLRSGAALRAPLPQGAAPARTQLGDEIPAIGRLLFSDYLLAFEALSFLLLVAA
                     VGALLLSKRRFE"
     gene            8438..8773
                     /gene="nuoK"
                     /locus_tag="PLB01_06380"
     CDS             8438..8773
                     /gene="nuoK"
                     /locus_tag="PLB01_06380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQQ76963.1"
                     /translation="MTGALPGVAPMAVPPGLYLVVGTLLFAIGLAGALLKRNALSIFL
                     SIELMMNAVNLLFLTYARMRGDMAGQMIVFFVIAIAAAEASIGLAIFVALFRARRTVD
                     VDKIALLKW"
     gene            8781..10862
                     /gene="nuoL"
                     /locus_tag="PLB01_06385"
     CDS             8781..10862
                     /gene="nuoL"
                     /locus_tag="PLB01_06385"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQQ76964.1"
                     /translation="MNQLWLIPVLPLAGFLLNGLVALLFASARSEKERDEWIKANPGA
                     ADGHGDHGAPGAHPAHDDHAHGGARLPYAQRLFHGVVGVGSVGLAAVLSFAALVPYVL
                     ESLKSHEGLAPVVQHAYTWMAAGDYSVDVAFRLDALSALMLSFVTFVGTLIHVYSVGY
                     MQDEEGYGRFFAYLNLFMFAMLTLVLADNLPLLFVGWEGVGLCSYLLIGYYYDKDFAA
                     AAGKKAFIANRVGDVGMVLGIFGIFALFGTADFSFVELAKNATLAPGSHISVYVVCLL
                     LFLGACGKSAQVPLYVWLPDAMAGPTPVSALIHAATMVTAGVYLVARCSAVFVLAPDA
                     LTLVAWIGAGTAIFAATIGLAQNDIKKVLAYSTVSQLGYMFLACGVGAFSAGMFHVFT
                     HAFFKACLFLGSGSVIMSMHHEQDMRKMGGLAARIPQTFRTMLVATVAIAGIAPLAGF
                     FSKDMILGSAAASGHWGLFAVGLFTAFLTAFYMFRLVSMTFWSSPRGDHHAHEHAHES
                     PATMTVPLWILAGLSVVAGFVGVPAVLGGSDRIGRFLEASVAHPELHEMSHGAELGLM
                     ALSLAAAFGGIALALSWYRRASGPEEAVSPQASSFYEKSGLLGRLVADKWNVDEGIEA
                     LILSPFRKIGTFLWRGFDALFIDGIANASAFLVELAGDLMRFFTTGHVRNYALAFSLG
                     ALVFVLYVWVR"
     gene            10867..12414
                     /locus_tag="PLB01_06390"
     CDS             10867..12414
                     /locus_tag="PLB01_06390"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQQ76965.1"
                     /translation="MNLHPLGADILTTLVFLPSLGALVLALLPGESKTAAKVVGIATS
                     LLTFAVSVVMFRSFDAAAPGIAGFAIDRPWIPRFGVRYQLGVDGLAVALVLLTTVLTV
                     SVLLVACGQKIDRFKGYVAAFLVLETGMLGSLVALDVLLFYVFWEVMLVPMYFIIGIW
                     GGKRRIYATMKFFLFTMAGSLLMFIAILYAAGVHSRATGLQSFSLADWTRAAASGAWG
                     LAPGVEALLFGAFALAFLVKVPLWPLHTWLPDAHVEAPTGGSIILAGVLLKLGTYGLL
                     RFAWPLFPHAAARYGPFIAVLALIGIVYGAWVAAAQKDMKKLVAYSSVSHLALVVLGI
                     VAGTGAALSGAIFQMIGHGLTTGLLFLLVGVLYERRHTREMADYGGVAARVPVTTALF
                     IIATLGSVGLPGLNGFIGEFLILGGVFQVSIAWAAIAATGMVLGAIYLLTLVQKVFWG
                     PETVPANRALTDINGWELAGAFPLIVLIVLLGVFPRPLLTLVDGSVKSLQKPAAAATA
                     AAVEAGR"
     gene            12411..13871
                     /locus_tag="PLB01_06395"
     CDS             12411..13871
                     /locus_tag="PLB01_06395"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HQQ76966.1"
                     /translation="MTGFLHVTGADFAAVGPEIVLCLAATLILLLDAFARPLRTYLPY
                     VALMSLAVANLAGRDVTGTFFAGAVEVTGFTRYMELVALGAVALAVLGGAAGLSRDKR
                     DQGEFYALLLFAAAGAILMVRGGDLLVVFIGLELMSLSLYVLAAWYRDNAAATEAGMK
                     YFLMGALASAFLLYGVATIYGRLGTTSFARLQGLASTPGLVAFDTMLAAGILAVVAGL
                     AFKIALVPFHGWAPDVYQGMTTPAVAFLSTAPKAGAVLVLARVLHALFPAGLGDPWRP
                     LLAILAVLSILFGNIVALSQRDLKRMLAYSGIAQMGYVTIAIATFTTDALEGAGVFLA
                     GYLVTNAAAFLAIAALSSGENEPKVLSELAGLGRRRPLAATVLSLSMLSLAGLPPTVG
                     FIGKLLVFRSAVEGGLVPLTIVGVFGSLVSVGYYLRVVYHLWMKEPVREVSLVPDDVL
                     SGAAFLLTAALMLLWGVFPRALIDVARQAATALLGR"
     gene            13874..14527
                     /locus_tag="PLB01_06400"
     CDS             13874..14527
                     /locus_tag="PLB01_06400"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HQQ76967.1"
                     /translation="MPVKSWDRPHGVPEATSERLSLVLRGLRALEAEGVHTVSSYQLA
                     DRFGLNPAQIRKDLAQFGEFGVRGVGYRVADLKAKLKQLMGLERTRTVVIAGAGNLGQ
                     ALADSRNFNVDGFKVAALFDVDGRKIGGRSRTGVPILDLKELPSNVATLKADIGVLAV
                     PAEAALEVARLVAGAGVPAILNFAPVSVPSLEGTFVRNVDLTFFLENLSFQLAVRRR"
     gene            complement(14534..17041)
                     /gene="glgP"
                     /locus_tag="PLB01_06405"
     CDS             complement(14534..17041)
                     /gene="glgP"
                     /locus_tag="PLB01_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQQ76968.1"
                     /translation="MRPLGTYRAAPALPPELAPLADLARDMRWTWRPATRALFRELDP
                     DEWRRSGNPVLLLRSVDPARLAALAGNADYVARVHEAAAAFAAEDAAAPGVPGIAALV
                     AAGERVAYFCAEFGLSEMLPIYSGGLGVLAGDHLKSSSDLGVPLVGVGIFYREGFFRQ
                     TLTADARQKESYPIVDPLDLPVEVLPTPAGVAPVVTVTIGDRDVHLLIRRVRVGRTPL
                     LLLDAHLPANRPADREITNRLYGGDIEMRIRQEVVLGIGGMRALEVAGLRPTVRHANE
                     GHAAFLGLERIRQLRAERGLSFAAAREIATAGNVFTTHTPVPAGIDLFPRDLMQRTFV
                     KKLESYGLDLDELLGLGRQVPEDPNEYFSMAVLGLRLSSRVNAVSQLHAKVSRRLWRG
                     VFPDAPLSEIPIGAVTNGVHGPSWTSKEIGALNPRDGDGPDPADLWRRHEHRRAALVE
                     TVRIRSAAARTRRGAPDVEVAAAWQLLDPTALTIGFARRFATYKRATLIFQDPARLSR
                     LLSDPNRPIQLVFAGKAHPRDHAGKEFLRRVGEFAAQPEFLGRVVLVEDYDMRLARRL
                     VQGVDVWLNNPRRPFEASGTSGMKAAMNGVLNLSVLDGWWDEAPRDGAGFTIGDATEG
                     RPDDEMANLLYERLESEIVPLFYDRPNGDRSGVPAGWAARMAASAHRLGRLFHSDRML
                     ADYVSRAYAPAAERVAMLSAEGDAKAKELATWKERVRAAWDEVGFVSVTTVPEEPARV
                     APGESFTVEVQIRLGGLEPADLAVDWFEGEPDHDGVVDAGFSAPLVSQGRLDGTATWA
                     GTVRRPEEDSRGYSVRVRPSHPLLVHPNETNLVLWAE"
     gene            complement(17067..17546)
                     /locus_tag="PLB01_06410"
     CDS             complement(17067..17546)
                     /locus_tag="PLB01_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76969.1"
                     /translation="MRIAVYCSDCMERLELPPPSDSASGEVSCSSPSRKSSISFSHSP
                     VVISGEKIDSCSRCKSTAFYVQKDFDQRVGCAVLALGAALALLAAWKLGGVWLVPVLL
                     VFAALDFVLARRVPPVVICYRCDTEYRDVPDAGSYKPYDPHVAERDAEVKTVRAMNP"
     gene            17681..>18163
                     /locus_tag="PLB01_06415"
     CDS             17681..>18163
                     /locus_tag="PLB01_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ76970.1"
                     /translation="MARSPDRNALAILAGAHAVNDAYQSAVPALLPFLVADRGLTYAA
                     AGGLVLAASAVSSFLQPLLGAASDRRALPSLMPLGILVAGAGIAGAALAPSYALTAAA
                     VVLSGAGVAVFHPEAARHANYASGDRRATGMSIFSVGGNLGIAAGPAAVTLLATAGGV
                     P"
BASE COUNT         2575 a         6610 c         6020 g         2958 t
ORIGIN      
        1 tcggcgacgg cgcggccgat gccccgggag gcgccggtca cgagcgcgac gcgcgtgcgc
       61 ggggcgtccg tcacgggatc accggagccg gcaccgcgcg cggaccggcc ggcaggacgg
      121 tcgcggcgaa gacgtgcacg tcctccggcc tctcgagcga gggccgcgcc acgatcgcgt
      181 cgcggagcgt ccaccggacg agcttcgtca ggacgtcgcc gggtccaacc tcggcgaagc
      241 gatgaatgcc cgcggctctc atggcgagga gcgtcggaga gaagaaagac ggaagagaga
      301 tttgctcaga aaggacatgg gcggcctccg cgctgctccc cacccggcgc cccagcatgg
      361 gagcaaagag aggagattgc ggctgcccca ccctcacacc cccttctaag aaaatcttca
      421 atttctcgct cacggactcg aggctcctcg agtgcatcgg gaacccgatc gtcagcatct
      481 ccgccttcag cgcccgcggc cgcatgaggt cgagggcctc ttccaccagg tcccgctctc
      541 ccgtcaggac gaactgcgcg gccgcgttct ccgtcgcgat cgcgaggcgc gagaggtcgg
      601 ggcagacgcc ggcgagggcc gcctcgacgt ccgcccgccc gaggccgatc acgaagccca
      661 tcgtcccgtc ggcctcgccg cccgcgcgct cctctgccag gagccgctcg gcctcgagca
      721 gggcgtcgag cgcggcctcg cgcgagagac acccggccgc gacgaacgcc gcgtacgtgc
      781 cgaggctgta gccgcagcag gcgtcgggga ctagaccgtg ctcgcggcga aggacctcga
      841 gtgcggcgag ggaaacggcg aagacgccga cctgggcggg cttgtcgtcg tggagggacg
      901 ggaggccctc gccaaaaaac gtctcggcga gccggactcc cgaccgggaa gagatttctt
      961 cgaagagtga ggagacgaag tcgtaccgcc tcgcgaaggc ctcgcccatc ccgggctttt
     1021 cgctgagctg gcccgggaac agcgcgccga acgtggcgcc gtggaaggcg tggccctccc
     1081 cggaaattcg cgccgcgttc atgcggcggc tccggagcgt gccgcgagcg gaggcgaaac
     1141 ggccgacgga ccgccgctgc cctcgcccct cttcccattc gcagaaatcc ccgggtgctc
     1201 ctcggacgcc gccgacgaga tcggcgcgaa cgtccagcgc ttcagccagc gccgccagcg
     1261 cgcttccgtc cgcccgcgcc ccgcgaagtg gacgaggcgc tgaacggtcg agaggcccgg
     1321 catctccggg tgctcggggt cgaactccca cggatgcgcg tagaggacgg gcgggatccc
     1381 ggctgcgagc gaccgctcga tcgccccctc gacgcgcctt tcgcgcgtca gtcgcgagca
     1441 gacgccgccg ccccacagcg cgcggcggcc gaagaacgtg cccatgaggg gcgggacctc
     1501 gaggatcggc cccgcggacg tcgcgaggac ggccggccgc accgggttcg ccagatcgcc
     1561 caccggcggc gccgtcgtga gcgaggagtc gatggcgaag ccttcctcca cgaggatggc
     1621 cagcgccggg ttgtcggcct tcagcatcga ccactccggg gcgcggaagg ccgagaccgg
     1681 ctttccggtg gcagcctgga gcgtgtcgcg gctccggcgc acgtcctcgc ggaactccgc
     1741 gggcgggagt tcgaagacgc ggcggtgcgt gtcgccgtgg cagccgatct cgtggccggc
     1801 ctcggcgatc cgtttcacga gagccgggct ctgccgggcg acccagccga ggacgaagaa
     1861 cgtcgccttc gacgccgttc cggcgagaag ctcgaggatc ctgtcggttc cgacgtggac
     1921 tcgcttctcg cgcgcggccc acgtcgcggg gtccgagtag gccgcgtgcc cgcacaggtg
     1981 aaaccactcc tccacgtcga cggtcaggat gggggcgggc atcggccgat tatgacgcgt
     2041 cggaatccag cgtcttgtaa tacacggaca gccaggacgt gatccacgtg tcgcggaacg
     2101 ggcggtagtg cgagcgcgcg ccgtagatcg tcttcacggg cacggcggcg aaccggcgga
     2161 cgcggggggc ggccttgatg aggatctcgt tctcgatgga ataccgctcg gcgcggaggc
     2221 gcagcgggcg cagcaggtcc gccgcgatca cgcggtagcc cgactgtccg tcctcgaccg
     2281 gcagcccggt catccggccc aggaccttgt cgccgatcgt gttcgcccag tagttcttcg
     2341 cgggcatcgc cgcggcgtcc ttgaggcgcg acccgatgac gaagtccgcc ccggccttcg
     2401 cggcgtcgag gagggcgggc aggtcggccg ggtcgtgctg gccgtcggcg tcgacgaacg
     2461 cgatgtgcgt gacgggcttc gcgaggagca cggcgagacc gtctctcaag gcggagcctt
     2521 tgcccccgtt cgcgggccgc acgaggacgc ggactccggc ggcgcgggcg gcgtcgccgg
     2581 tgccgtcctc gctcccgtcg tccacgacca ggacctcggg gacgacgcgt ttcagccccg
     2641 tgaccacgcc gcccaccgtg gaggcgcatc ggaaggcggg cacgagggcg gcggggcgaa
     2701 acccttcgta attcttctcg gacatgacgg ttggggattg acgggagtgc cctgtttgtg
     2761 aaaagtatca cgagcgacga atgggcagcc cgctggcctt ccttccgatc ctgatgatgc
     2821 tcgtgggcgc catgggcgtc ggagccctgc tcctcgggct gaactacctc ctcggccgca
     2881 aggtgaagac gaaacgcaag ctctccacct acgagagcgg catgcccctc ctcgacgaca
     2941 gccacaagcg catcagcgtg aagttcttca tcgtcgcgat gatcttcatc ctgttcgaca
     3001 tcgaggccgc gttcctctac ccgtgggcgg tcgtgttccg cgagggcgga atggccgtgt
     3061 tcgtggagat gctcgtcttc gtcgtcgtgc tcttcctcgg cttcggctat ctctggaaga
     3121 agggcgcctt cgattggaac tgatgtgagc ggcggagcga acgtcccggt ggccgagcag
     3181 gccggacttc cccgcaccgg ggagcacggc gccctctcct gcgggaaggc cctgaccttc
     3241 tcgaaggaag cgcgcgccga gctcgaccgc ctcctgacac gctatccgac gaagcaggcc
     3301 gccctcctcc cggcgctctg gctcgcccag aaggaatggg gctggatctc gcgcgaggcg
     3361 gtggaccacg tcgcctcggt tctcgacctc tcgccggcgt tcgtgtgggg cgtcgtgacg
     3421 ttctacacga tgtacaaccg cgcgccggtc ggcaagaacc tcatccaggt ctgcacgtcc
     3481 atctcgtgcc acctcaacgg cgccgaagag gtgtttcacg agtgcaagaa gcggctcggg
     3541 gacctgaaga ccggcgagac gtcgaaggac gggaagtaca cggtcatcga ggtcgagtgc
     3601 ctcgcccagt gcgacaaggc gccggccgtg atggtcaacg acgacgacta cgccgtgatc
     3661 acgaaggaca agctcgacgc gttcctgaag gggctgggct gaggcgatga gcggactcga
     3721 gacgattccc cccgtcctcc tcgcccgcct cggaaagccc gactcccgcg cgatcgacgg
     3781 ctacgtcgcc gacggcggct acacctcgtg gaagaaggtc ctcgccggcg cgaagaccgg
     3841 cgagtggaca cccgcgaagc tgacggacat ggtcaaggcg tcgggcctgc gcggccgcgg
     3901 cggcgccggc ttcccgtgcg ggctgaagtg gacgttcgtg cccgcgaagg acaagcgcgg
     3961 cgaaaagccc gtctacctcc tctgcaacgc ggacgagtcc gagcccggaa cgttcaagga
     4021 ccggctcctc atcgagcagg acccgcacca gatcctcgaa gggatgatgc tcgcgagtta
     4081 cgccctcgac gtgaagcacg cgtacatcta catccgcggc gagatggtcc acggcgccga
     4141 gtacctcaac gcggccgtcg cagaggcgca cgccaagggc ttcctcggga ccgacattct
     4201 cggctccggc atcgacctga cgatcaccgt ccaccggggc gcgggcgcct acatctgcgg
     4261 ggaggagacg gccctcatcg agagcctcga gggcaagcgc ggccacccgc gcatcaagcc
     4321 gcccttcccc gccgtctccg gcgtctgggg cgccccgacg gtcgtcaaca acgtcgagac
     4381 gctctgctgc gtgaagcaca tcgtcgaccg cggcgcggac tggttcaagg gcgtcggccg
     4441 caacgagaag aacaccgggc cgaagctcta tgccgtctcc ggccacgtcg agaagcccgg
     4501 cgtctacgag gcgccgatcg ggatcccgtg ggagcagctg ctcgcgatgg cgggcggcgt
     4561 gcgcggcggg cgaaagctca aggccgtgat tcccggcggc tcctcggcgc ccattctcac
     4621 ggccgccgag atcgcgggca tcccgatgga cttcgacggc atcgccgccg cgaagtcgat
     4681 gttcggctcg gcggcgatca tcgtgatgga cgagacgacc gacatcccga aggccgtcac
     4741 gaacatcgcg aagttctacg cgcacgagtc ctgcggccag tgcacgccct gccgggaggg
     4801 cacgccgtgg ctcctcaaga tgctccggcg gatcgtgaac ggagagggca agaaaaccga
     4861 cctcgacctc ctcctccaga tctcgaacca gatgggcaac ggcatgacga tctgcgtctt
     4921 cgccgacgcc gccatcgcgc ccgtcatctc gggcatcgtg aagttccggg gcgagttcga
     4981 ggcgtacatc gagaaatccc agaagcccac ctttgaacag ggcgcctccg acgacctcac
     5041 gaacgcaatc gccgaccgca ccgccgcagg gattcattct tagaatgagt aagaacgctc
     5101 cctcctcctc ttacctttct aagaaactct tctcttcatc ctcgttctct acggacgtga
     5161 catgcccaag gtaacggtgg acggtcgcac cgtcgaggtg cctgcggacg cgaacgccct
     5221 cgaggcgtgc cgcgcggcgg gcgtcgacgt cccgcacttc tgctaccacc cggcgctttc
     5281 catcgtcggc cagtgccgca tgtgcatggt cgaggtcgaa ggcgtgccga agatccaggc
     5341 ttcgtgcacg gtgcccgtgc gcgacgggat ggtcatcaag gcgaacacgg agaaggccct
     5401 cgcggcgcgc aacgccacga tggagttcct cctcatcaac cacccgctgg actgccccat
     5461 ctgcgaccag gccggcgagt gcaagctgca ggacaacgcg gtcgccggcg gcctgcccgt
     5521 ctcgcgcacg tccgagccgc gccgccagtt ccccggctac gaccgcacgg acatcggccc
     5581 tcacgtgatc gcggacatga cgcgctgcat ccagtgcacg cgctgcatcc gcttctgcca
     5641 ggagatcacc gggaccggcg agctcacctt catcgagcgc ggcggccaca cgatcgtctg
     5701 gacgcacgag ggcaaggcgc tggacaacga cctttcgggc tgcgccgcgg acgtctgccc
     5761 cgtcggcgcc ctcacgacga aggagttccg gttccgcaag cgcgtctggt ggctcgacaa
     5821 gacccgctcg atctgcgacg gctgcgaaat cggctgcagc ctctcgatcg agcaccgtga
     5881 cggcgtcgtc taccgctaca cgccgcgcgt ccagcccgcc gtcaacgact actggctctg
     5941 cgactacggc cgcttccggg ccgaggatct caacgcccgg gacttcacga agcccgaggt
     6001 ccgggtcgcc ggtgcgaata cgcgaaagca gacgaactgg ggcgaggcgc tcgacgcggt
     6061 gaaggccgcg atcgagaagg cgaaggccga agagaagagt tcttcgaaag tgatggtcat
     6121 cgggtcggct cacctcacca ccgaggaggc cttccttctc gccacgctct gcaaagattc
     6181 tttgaaggtg aatgcacccg agttcaccgt ggacatcggg cctaagaggc gcatccggaa
     6241 caagaaggac ggctggctct acggaaccga ggcggcgccc aacagcaggg gctgcgaagc
     6301 ggcgggcctc acccgagcca aagaagccgg gacgtccccc ctgtccctct tactttcaaa
     6361 gaaatcttcc tcttctccct cggtcctcta catcaccgac gccgcattca cggaatgggc
     6421 gaacgacccg gccttcgtgg ccgagctgcg caaggcctcc gtcctgatcg tccacgcccg
     6481 caagcgcaat gcgctgacgg acgtggcgga catcgtcctc ccggtggcgt cgctcgccgg
     6541 caccgagggg acgttcgtga acgcgaaggg ccgtgtgcag cgcgtcgacc tcgcgatcct
     6601 cgcgccgccc atcgtgcgca cgaacctgga gatcttcctg catctcggcg cgaagtacgg
     6661 cgccttcgac acgcagtgga ccgcccgcga cgtcttcgac cggatgacgg cgtcggtcgc
     6721 cggctatgcc ggcctcgact gggactccga cgacctcgtc ggcaacccgc cgtccgtctc
     6781 ccccgccgcc gcctacgacg ccctcggcct gaacaaggac gccgacgtcg aggtcttcac
     6841 gcgcccgtcc gtcgcggcgc cggaccgcaa gtcgtgagcc cgacgctctc ggccgccctc
     6901 gagggagccg gcaagatcgc cttcatctgg ggcgtcgtcc tcgcggccat gctcccgaac
     6961 ctcatctgga tggagcgccg catcgccggc ctcatccagg accgacccgg tccgaaccgc
     7021 gtcggcccgt tcggcctctt ccaggccctc gcggacgtcc tcaagatgtt catgaaggag
     7081 gacatcgtcc cgctctacgc ggaccgcctc ctccacacgc tcgcgccgtt catcctgctc
     7141 gtcccggcga tggcgacgtt cgccgtgatc ccgttcggct cgaagatccc gctcttcggg
     7201 cgcgagatcc cgctcgtcgt cgccgacgtg aacatcggga tcctctacgt gttcgcgctc
     7261 gcctccgtcg gtgtgtacgg cctcgtgctc gccggctggg cgtcgaacaa caagttcgcc
     7321 ctcatgggcg gcatccgctc gtccgcgcag gtcatcagct acgagctctc gatgggcctc
     7381 gcggcggtcg cgacgctcat ggccgcgggc tcgctgcgcc tcacgcgcgt cgtcgagtac
     7441 caggcgggcg gcacgttcct cggattcctt ccgctctgga actgcttcac ccagccgctc
     7501 ggctgcatca tcttcatcat cgcggcgttc gccgagacga accgcatccc gttcgacttg
     7561 cccgaggccg aggccgagct cgtcgcgggc tatcacacgg agtactcggg cttccggttc
     7621 ggcgcgttct tcatggccga gtacctgaac ctcgtcgcgt cctcgatgat gatggcgacg
     7681 ctctacttcg gcggctggtc gctgcccttc ctcccgcact ccggcctcgc gggcggcctc
     7741 ctctcggtcg ccgtcttctt cgcgaagacg atgtgcttcg tcttcctctt catgtgggtc
     7801 cgctggaccc tgccccgctt ccgcttcgac cagctcatgg acatcggctg gaaggcgctc
     7861 ctcccgctgt cgctcctcaa cctcctctgg acgggggcgc tcgtcctcct gaaggtgatc
     7921 tgatgccggc cctcagcgga gagctcgtcg tcttcacgat cgcgtccgtg ctcgcgatcg
     7981 ggttcgccct ggcgatggtc gtccagaaga cgcccgtccg gagcgtcctc tcgctcgtcg
     8041 tgacgttctt cggtctcgcg atcttctcga tccagctcgc ggcgcccttc atcgcggtcc
     8101 tgcaggtctt cgtctacgcg ggcgcgatcc tcgtcctgtt cctcttcgtc atcatgctgc
     8161 tcaacctgag ccaggaagcg acggaggagg actcccggcc cgtccagcgg tggctcggcc
     8221 tcgtggcggc gctcgcgctc ggcggcctcc gctccggcgc ggccctccgg gcgcccctgc
     8281 cccagggcgc cgcgcccgcg cggacccagc tcggcgacga gatcccggcg atcggccgcc
     8341 tcctcttctc cgactatctc ctcgcgttcg aggcgctgtc cttcctcctc ctcgtggccg
     8401 ccgtcggcgc gctgctcctc tcgaagagga ggttcgaatg accggcgccc tgcccggcgt
     8461 cgccccgatg gccgtgccgc ccggccttta cctcgtcgtc gggacgctgc tcttcgcgat
     8521 cggcctcgcg ggcgccctcc tcaagcgcaa cgcgctctcg atcttcctgt cgatcgagct
     8581 catgatgaac gccgtcaacc tcctgttcct cacgtacgcg cgcatgcgcg gcgacatggc
     8641 gggacagatg atcgtcttct tcgtgatcgc gatcgcggcc gccgaggcct cgatcgggct
     8701 cgcgatcttc gtcgcgctct tccgcgcccg ccgcacggtg gacgtggaca agatcgcgct
     8761 cctgaagtgg taggccgcgc atgaaccagc tctggctcat cccggtcctc cccctcgcgg
     8821 gattcctcct gaacggcctc gtggcgctcc tcttcgcgag cgcccgctcc gagaaggaac
     8881 gcgacgagtg gatcaaggcg aacccgggcg cggccgacgg ccacggtgac cacggagctc
     8941 ccggcgctca tcccgcgcac gacgaccacg cgcacggcgg cgcccgcctc ccgtacgcgc
     9001 agcgcctgtt ccacggagtc gtcggcgtcg gctcggtcgg cctcgcggcg gtcctctcgt
     9061 tcgcggccct cgtcccgtac gtcctcgagt ccctgaagag ccacgagggc ctcgctcccg
     9121 tcgtccagca cgcctacacg tggatggcgg cgggcgacta ctccgtcgac gtcgcgttcc
     9181 gtctcgacgc gctctcggcg ctcatgctct cgttcgtcac gttcgtcggg acgctcatcc
     9241 acgtctacag cgtcggctac atgcaggacg aggagggcta cgggcgcttc ttcgcgtacc
     9301 tgaacctctt catgttcgcg atgctcacgc tcgtcctcgc ggacaacctc ccgctcctct
     9361 tcgtgggctg ggagggcgtc gggctctgct cctacctcct gatcggctac tactacgaca
     9421 aggacttcgc ggccgcggcc ggcaagaagg cgttcatcgc gaaccgcgtg ggcgacgtcg
     9481 gaatggtgct cgggatcttc gggatcttcg ccctcttcgg gacggccgac ttctcgttcg
     9541 tcgagctcgc gaaaaacgcg acgctcgccc ccggctcgca catctccgtc tacgtcgtct
     9601 gcctcctcct cttcctcggc gcgtgcggca agagcgccca ggtcccgctc tacgtctggc
     9661 tgccggacgc catggccggc ccgacgcccg tctcggccct catccacgcc gcgacgatgg
     9721 tcacggccgg cgtctacctc gtcgcgcggt gcagcgccgt cttcgtcctc gcgcccgacg
     9781 ccctcacgct cgtcgcgtgg atcggcgcgg gcacggcgat cttcgcggcg acgatcggcc
     9841 tcgcccagaa cgacatcaag aaggtcctcg cctactcgac ggtctcgcag ctcggttaca
     9901 tgttcctcgc ctgcggcgtc ggcgccttct cggcgggcat gttccacgtc ttcacgcacg
     9961 ccttcttcaa ggcctgcctg ttcctcgggt cgggctccgt gatcatgtcg atgcaccacg
    10021 agcaggacat gcggaagatg ggcggcctcg ccgcgcgcat cccgcagacc ttccggacga
    10081 tgctcgtcgc gacggtcgcg atcgcgggca tcgcgcccct cgcgggcttc ttctcgaagg
    10141 acatgatcct cggctccgcg gccgcctccg gccactgggg cctcttcgcc gtgggtctct
    10201 tcacggcatt cctgacggcc ttctacatgt tccgcctcgt ctcgatgacg ttctggtcct
    10261 cgccgcgcgg cgaccaccac gcccacgagc acgcccacga gagcccggcc acgatgacgg
    10321 tcccgctctg gatcctcgcc ggcctgtccg tcgtcgcggg cttcgtcggc gtccccgcgg
    10381 tcctcggcgg ctccgaccgg atcgggcgct tcctcgaggc ctccgtggca caccccgagc
    10441 tgcacgagat gtcgcacggc gccgagctcg gcctgatggc gctctcgctg gccgccgcgt
    10501 tcggagggat cgcgctcgcc ttgagctggt accggcgcgc atccggtccc gaagaagcgg
    10561 tgtcgccgca agcatcttct ttctatgaaa aatccggtct cctcggacgt ctcgtcgccg
    10621 acaagtggaa cgtcgacgag gggatcgagg cgctcatcct ctcgccgttc cggaagatcg
    10681 gcacgttcct ctggcgcggg ttcgacgcgc tgttcatcga cgggatcgcg aacgcctcgg
    10741 cgttcctcgt cgagctcgcc ggcgacctca tgcgcttctt cacgaccggc cacgtccgca
    10801 actacgcgct ggccttctcc ctgggcgctc tcgtcttcgt tctctacgtg tgggtgaggt
    10861 agcgcgatga acctccaccc gctcggcgcc gacatcctca cgacgctcgt cttcctcccc
    10921 tcgctcggag cgctcgtcct cgcgctcctc cccggagaat cgaagacggc cgcgaaggtc
    10981 gtggggatcg cgacatcgct cctcacgttc gccgtcagcg tcgtcatgtt ccggagcttc
    11041 gacgcggcgg cgcccggcat cgcgggcttc gcgatcgacc gcccctggat cccgcgcttc
    11101 ggcgtccgct accagctcgg cgtcgacggc ctggccgtcg cgctcgtcct cctcacgacg
    11161 gtcctcacgg tctcggtgct gctcgtcgcg tgcgggcaga agatcgaccg gttcaagggc
    11221 tacgtcgccg cgttcctcgt cctcgagacg gggatgctcg gcagcctcgt cgcgctcgac
    11281 gtcctcctct tctacgtgtt ctgggaagtc atgctcgtcc cgatgtactt catcatcggg
    11341 atctggggtg ggaagcgccg catctacgcg acgatgaagt tcttcctctt cacgatggcg
    11401 ggctcgcttc tgatgttcat cgcgatcctg tacgccgccg gcgtgcacag ccgcgcgacg
    11461 ggtcttcaga gcttctcgct cgcggactgg acccgcgcgg cggcgtcggg cgcctgggga
    11521 ctcgcgccgg gcgtcgaggc ccttctcttc ggcgctttcg cgctcgcgtt cctcgtgaag
    11581 gtcccgctct ggccgctgca cacgtggctg cccgacgccc acgtggaggc gcccacgggc
    11641 ggctcgatca tcctggcggg cgtccttctc aagctcggga cgtacggcct cctgcgcttc
    11701 gcctggccgc tcttcccgca cgccgccgcc cggtatggac cgttcatcgc cgtcctcgcc
    11761 ctgatcggga tcgtctacgg cgcatgggtg gccgccgcgc agaaggacat gaagaagctc
    11821 gtcgcctatt cctccgtgag ccacctcgcg ctcgtcgtcc tcggcatcgt cgccgggacg
    11881 ggcgccgcgc tctccggggc gatcttccag atgatcggcc acggcctcac gacgggactc
    11941 ctcttcctcc tcgtcggcgt cctctacgag aggcgccaca cgcgggagat ggccgactac
    12001 ggcggcgtcg cggcgcgcgt tcccgtgacg acggcgctct tcatcatcgc cacactgggt
    12061 tccgtgggcc tccccggcct gaacggcttc atcggcgagt tcctgatcct cggcggcgtc
    12121 ttccaggtct cgatcgcctg ggccgcgatc gctgcgaccg ggatggtcct cggcgcaatc
    12181 tacctgctga ccctcgtcca gaaggtcttc tggggccccg aaaccgttcc cgcgaatcgc
    12241 gccctcacgg acatcaacgg ctgggagctc gcgggcgcct tccccctcat cgtcctgatc
    12301 gtcctcctcg gcgtcttccc gcggccgctc ctgacgctcg tggacggctc ggtgaagtcc
    12361 ctccagaagc ccgctgcggc ggcgacggcc gcggcggtgg aggccggccg atgaccggct
    12421 tcctgcacgt caccggagcg gatttcgccg cggtcgggcc ggagatcgtc ctctgcctcg
    12481 cggcgacgct catcctcctg ctcgacgcgt tcgctcgccc cctgcgcacg tacctgccgt
    12541 acgtcgcact catgtctctc gcggtcgcga acctcgcggg ccgcgacgtc acggggacgt
    12601 tcttcgcggg cgccgtggag gtgacggggt tcacgcgcta catggagctc gtcgccctcg
    12661 gggccgtggc gctcgccgtt ctcggcggcg ccgcgggact ttcgcgcgac aagcgcgacc
    12721 agggcgagtt ctacgccctc cttctcttcg ccgcagcagg agccatcctc atggtgcgcg
    12781 gcggggacct cctcgtcgtc ttcatcgggc tcgaactcat gtcgctcagc ctctacgtcc
    12841 tcgccgcctg gtaccgcgac aatgcggccg cgaccgaggc agggatgaag tacttcctca
    12901 tgggcgccct cgcgtcggcg ttcctcctct atggcgtcgc gacgatctac ggtcggctcg
    12961 ggaccacgtc gttcgcgcgg ctccagggac tcgcgtcgac gcccgggctc gtggcattcg
    13021 acacgatgct cgcggcgggc atcctcgccg tcgtcgcggg tctggccttc aagatcgcgc
    13081 tcgtcccgtt ccacggatgg gcgccggacg tctaccaggg catgacgacg cccgccgtcg
    13141 cgttcctgtc gacggccccg aaggcaggcg cggtgctcgt cctcgcgcgg gtcctccacg
    13201 cgcttttccc cgcggggctc ggcgacccgt ggcggccgct cctcgcgatc ctcgcggtgc
    13261 tttcgatcct gttcgggaac atcgtcgccc tctcgcagcg cgacctcaag cgcatgctcg
    13321 cgtactcggg gatcgcgcag atgggatacg tgacgatcgc gatcgccacg ttcacgacgg
    13381 acgccctcga gggcgccggc gtcttcctcg ccgggtacct cgtgacgaac gcggcggcct
    13441 tcctcgcgat cgcggcgctc tcgtccggag agaacgagcc gaaggtgctc tcggagctcg
    13501 cgggcctcgg ccggcggcgc ccgctcgccg ccacggttct ctccctctcg atgctctcgc
    13561 tcgcgggcct cccgcccacg gtcgggttca tcggcaagct cctcgtcttc cgctccgccg
    13621 tcgagggcgg cctcgtcccg ctgacgatcg tcggcgtctt cggcagcctc gtgtccgtgg
    13681 ggtattacct ccgcgtcgtc taccacctct ggatgaagga gccggtgcgc gaagtttccc
    13741 tcgtgccgga cgacgtcctc tcgggggcgg cattcctcct gacggccgcg ctcatgctcc
    13801 tctggggcgt gttcccgcgc gccctgatcg acgtggcccg ccaggccgcg acggccctgc
    13861 tcggccgctg acgatgcccg tgaaatcctg ggaccgtccc cacggggtcc cggaagccac
    13921 cagcgagcgg ctctcgctgg tcttgcgcgg attgcgcgcg ctcgaggccg agggcgtcca
    13981 cacggtctcc tcgtaccagc tcgcggaccg gttcgggttg aacccggcgc agatccggaa
    14041 ggacctcgcg cagttcggcg agttcggcgt gcgcggcgtc ggctaccgcg tcgcggatct
    14101 caaggcgaag ctcaagcagc tcatgggcct cgagcggacg cgcaccgtcg tgatcgcggg
    14161 ggcgggcaac ctcgggcagg ccctcgcgga ctcgcgcaac ttcaacgtgg acgggttcaa
    14221 ggtcgcggcg ctcttcgacg tggacggacg caagatcggc gggcgctccc ggacgggcgt
    14281 cccgatcctc gacctgaagg agctcccgtc gaacgtcgcg acgctcaagg ccgacatcgg
    14341 cgtcctcgcg gttcccgccg aggcggctct cgaggtggca cgcctcgtcg cgggcgcggg
    14401 cgtgccggcc atcctcaatt tcgcgccggt ctcggttccc tccctcgaag gcacgttcgt
    14461 gcgcaacgtc gacctgacgt tcttcctcga gaacctctcc ttccagctcg ccgtccggcg
    14521 gcgctaggcg cgcctactcg gcccagagca cgagattggt ctcgttcggg tggacgagga
    14581 gcgggtgcga cggccggacg cgcacggagt acccgcggct gtcctcctcg ggacgccgca
    14641 cggtgccggc ccacgtcgcg gtgccgtcga gccgcccctg agagacgagc ggcgccgaga
    14701 agcccgcgtc cacgactccg tcgtggtccg gctcgccctc gaaccagtcc accgcgaggt
    14761 cggccggctc gaggcctccc agtcggatct ggacctcgac cgtgaagctc tcccccggcg
    14821 cgacacgtgc cggctcctcg ggaaccgtcg tcaccgagac gaacccgacc tcgtcccacg
    14881 cggcgcggac tctctccttc cacgtggcga gctccttcgc cttcgcgtcg ccctccgccg
    14941 agagcatcgc gacgcgctcc gccgccggcg cgtacgcgcg cgaaacgtag tcggcgagca
    15001 tccggtccga gtggaagagg cgccccagcc ggtgcgccga cgccgccatg cgcgccgccc
    15061 agccggccgg cacgcccgac cggtcgccgt tcggccggtc gtagaacagc ggcacgatct
    15121 cggactcgag gcgctcgtag agcaggttcg ccatctcgtc gtccgggcgg ccctccgtcg
    15181 cgtcgccgat cgtgaagcct gcgccgtcgc gtggcgcctc gtcccaccag ccgtccagga
    15241 ccgacaggtt caggacgccg ttcatcgcgg ccttcatgcc cgacgtcccg gacgcctcga
    15301 acggccgccg cgggttgttc agccagacgt cgactccctg cacgagacgg cgcgcgaggc
    15361 gcatgtcgta gtcctcgacg aggacgacgc ggcccaggaa ctcgggctgc gcggcgaact
    15421 cgccgacgcg ccggaggaac tccttgccgg cgtggtcgcg cgggtgggcc ttccccgcga
    15481 agacgagctg gatggggcgg ttcgggtccg acaggaggcg cgagagccgc gccggatcct
    15541 ggaaaatgag cgtcgcgcgc ttgtacgtcg cgaagcggcg cgcgaacccg atcgtcaggg
    15601 ccgtcggatc gaggagctgc caagcggcgg cgacctcgac gtccggagcg ccccggcgcg
    15661 tccgggccgc ggcggacctg atccgcacgg tctccacgag agccgcccgg cgatgctcgt
    15721 gccgccgcca gagatccgcg ggatcggggc cgtccccgtc gcgggggttg agagccccga
    15781 tttccttgga ggtccacgag gggccgtgga cgccgttcgt cacggcgccg atcgggatct
    15841 ccgagagcgg cgcgtccggg aacacgccgc gccacaggcg gcgcgacacc ttcgcgtgga
    15901 gctgggacac ggcgttcacg cgggacgaca ggcgcaggcc caggaccgcc atcgagaagt
    15961 actcgttcgg atcctccgga acctgcctgc cgaggccgag cagctcgtcg aggtcgaggc
    16021 cgtaggactc gagcttcttg acgaacgtgc gctgcatgag gtcccgcggg aatagatcga
    16081 ttccggcggg caccggcgtg tgcgtcgtga agacgttgcc cgcggtcgcg atctcgcgcg
    16141 ccgccgcaaa cgacaggccc cgctcggcgc ggagctggcg gatccgctcg agcccgagaa
    16201 acgccgcgtg cccctcgttc gcgtgtcgca ccgtcggccg gagccccgcg acctcgaggg
    16261 cgcgcatccc gccgattccg aggacgactt cctggcggat ccgcatttcg atgtcgccgc
    16321 cgtagagccg gttcgtgatc tcgcggtccg cgggccggtt cgcgggcagg tgcgcgtcga
    16381 ggagaagaag cggcgtgcgc ccgacgcgca cgcgccggat caggaggtgg acgtcgcggt
    16441 cgccgatggt gaccgtgacg accggcgcga cgcccgcagg cgtgggaaga acctcgaccg
    16501 gcaggtcgag cgggtcgacg atcggatagc tctccttctg ccgagcgtcc gcggtgagcg
    16561 tctgccggaa gaagccctcg cggtagaaga tcccgacacc gacgagcggc acgccgaggt
    16621 cgctcgagga cttcaggtgg tcgcccgcga ggacgccgag gccgcccgag tagatcggga
    16681 gcatctcgga gaggccgaat tcggcgcaga agtacgcgac gcgctcgccc gcggccacga
    16741 gagccgcgat cccgggaacg cccggcgcgg cggcgtcttc cgcggcaaac gcggccgcgg
    16801 cctcgtggac gcgcgcgacg tagtccgcgt tcccggccag cgccgcgaga cgcgccgggt
    16861 ccacggatct gaggagcagg acggggttgc ccgagcggcg ccactcgtcg gggtcgagct
    16921 cgcggaagag ggcgcgggtc gccgggcgcc acgtccagcg catgtcgcgg gcgaggtcgg
    16981 cgaggggggc gagttccggc ggcagggcgg gcgcggcgcg gtacgttccg agaggtctca
    17041 tcggcgcgcg gaggataacc gatcggtcag gggttcatcg cccggaccgt cttgacctcg
    17101 gcgtcgcgct cggcgacgtg gggatcgtac ggcttgtacg agcccgcgtc cggcacgtcg
    17161 cggtattccg tgtcgcaccg gtagcagatg acgacgggcg gcacgcgccg cgcgagcacg
    17221 aaatcgagcg ccgcgaacac gagcaggacg ggcacgagcc agacgccgcc gagcttccac
    17281 gccgccagga gcgcgagcgc cgcgccgagc gcgaggaccg cgcagccgac gcgctggtcg
    17341 aagtccttct ggacgtagaa ggccgtcgac ttgcatcgcg agcacgagtc gatcttttct
    17401 ccggagatga cgaccgggga atgggagaag gaaattgaag atttcctaga aggtgaagag
    17461 cagctcacct cgccggatgc gctgtccgaa gggggcggga gctcgaggcg ctccatgcag
    17521 tcagagcaat acacggcgat gcgcaagaac gaagcatagt ggacgggggc gtcgcgcgcc
    17581 gccagacgcc gtcgcgcgcc gccgcttcct cctgccctcc tcaccttcaa agaaaatctt
    17641 ctcttctctt cctctctcac catacgatcc ctgtcttccg atggcccgct cccccgaccg
    17701 caacgccctc gcgatcctcg ccggcgctca cgccgtcaac gacgcgtacc agagcgccgt
    17761 gccggcgctg ctgccgttcc tcgtcgccga ccggggcctc acctacgcgg ccgccggcgg
    17821 cctcgtcctc gcggcgtccg ccgtgtcgtc cttcctccag ccgcttctcg gggcggcctc
    17881 ggaccgcagg gccctgccgt ccctgatgcc tctcggaatc ctcgtcgcgg gcgccggcat
    17941 cgccggcgcc gccctcgccc cgagctacgc gctcacggcg gccgcggtgg tcctctcggg
    18001 cgcgggcgtc gccgtctttc acccggaggc cgcccgccac gcgaactacg cgtcgggcga
    18061 ccggcgcgcg accgggatga gcatcttctc cgtcggcggc aacctcggga tcgccgcggg
    18121 gccggcggcc gtcacgctcc tcgcgaccgc cggcggagtg ccc
//
LOCUS       DAOMFY010000052        18079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_970_length_18079_cov_7.267921, whole genome shotgun sequence.
ACCESSION   DAOMFY010000052 DAOMFY010000000
VERSION     DAOMFY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18079
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_970_length_18079_cov_7.267921"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1859)
                     /locus_tag="PLB01_06420"
     CDS             complement(297..1859)
                     /locus_tag="PLB01_06420"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76971.1"
                     /translation="MPESMRVGILAAALLPLSLAAGAPEERGAQPAANQGAASADGGM
                     VVESHASNTRTALASGRAPLIGFIDSQAPYCTQPDPRQDSCYVNVAYTSVSASPNYMV
                     NLHTFIDNKLVFRAQGFFQTSLNIDASNAGLGFRVACGPPRDDPGDTGTPPKRQVGNS
                     YSLTVRAEDSAGLKSANYATVTCPPHYTANGAPVIAVTPPSLASTQAPGTQSVAKLTI
                     GNTAAFPLWWNLPVSPTSGCGSAGVPWLAAGLPSGSTGPLASEDVPVTFNSTGLAPGH
                     YAAHVCVQSGDTFSPQIDVPVALTVTCPLGPPLAITASPTVGAGSPGRVASVRPNAGS
                     TFTWTITNGTITAGQGTNTVTFTAGTAGTPVGLGVTETNPDPAVCIGAAATASIPVLP
                     AGSSLLFFPVTPCRLADTRLVAGPLGGPVLAPSEVRLLKATASCNIPPGALGVSMNVT
                     VTQPAASGFFAAYPGTAAYPGTSTLSFRAGQTRADATVIGLATDGSGTFRILNGSAGT
                     AHVVIDVNGYFY"
     gene            complement(1890..2447)
                     /locus_tag="PLB01_06425"
     CDS             complement(1890..2447)
                     /locus_tag="PLB01_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76972.1"
                     /translation="MSLTRALATFALLPFCLAAAPPGERAAQAAAKPVAPPPDGGMVV
                     ESHGSSTRTALATGRTPLISFIDSQAPYCTQPDPRQDACFVNVAYTSVSAAPNYMVYL
                     HMFIDDKLVFRAQGFFQTSLYVSASNAGLGFRVACGPPRDDPSDTAVPKRQVGNSYSL
                     TVRAEDSATLKSANYATVTCPPYSP"
     gene            complement(2494..3894)
                     /locus_tag="PLB01_06430"
     CDS             complement(2494..3894)
                     /locus_tag="PLB01_06430"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HQQ76973.1"
                     /translation="MIRRQTRRLQAVFLTADVLATVLALWAAWLIRFESVWPTPKGGQ
                     PFGNYTPLIPILLVVWPVVFYFHRLYQIRRDRSAIDEVLSILVAATLGTILLVGILSF
                     WRGFTYTSRQLLVLFLVLDIVLVSLARFGIRKYLETMWATGAGVRRVLIVGAGHSGRA
                     LADKLLEHPATGMKPVGFADDDPAKRRENYRGLNMLGTTGEVRDLIRAHEVDTVFLAL
                     PVEAHRTMLAILKDVGNEMIDLRVVPDLFQYVTFKAGVEDFDGLPVINLTQMPLEGWN
                     SLVKRTMDVVLSAFGLVALAVLFPFIALAIWLEDRGPVFYAQERMGLDGRLFRMIKFR
                     SMRVDAEDEGAQWTQENDPRRTRVGAFLRKTSLDELPQLVNVFTGDMSLVGPRPERPE
                     FVKEFKEKFPQYMLRHRVRAGITGWAQVHGWRGNTPIDKRIEYDLYYIENWSVGLDVK
                     ILWATVRWGFTHKNAY"
     gene            complement(3891..5018)
                     /locus_tag="PLB01_06435"
     CDS             complement(3891..5018)
                     /locus_tag="PLB01_06435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ76974.1"
                     /translation="MRVALVHDWLTGTRGGEKVLLELVRMFPDAPVFTLFHFPGSQHP
                     EIEAREIRTTFLQSLVSPSRNYRPLLPLFFAAAESWDLSGYDLVISTSHCVAKNAKKD
                     AGAFHLCYCHTPVRYLHDQFDEYFRGRPAAVVAAARLVRAPLRAWDVATVPRVDAFLA
                     NSANVKDRIARLWRRDAAVVSPPVDTEFYTPGPGGERRGLLVVSALAPYKRLDDAIAA
                     ANVRKLPLTIAGFGPESRRLEAMAGETVVFADTPNDTMLRQLYRSAEAVLMPGEEDFG
                     IVPLEAQACGTPVIALGRGGALETVRDGETGVLMPEPGAASLLAAFDRLRSLRLDPAA
                     AVANAARFSREAFRGRFRRALDEALARPAASPSKMPPPVTV"
     gene            complement(5015..6220)
                     /locus_tag="PLB01_06440"
     CDS             complement(5015..6220)
                     /locus_tag="PLB01_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQQ76975.1"
                     /translation="MTRPRVLVDARKAADFGIGSYIRGLLGGLVRQERWDLCATVHPG
                     GDALLPPGVSPVACDARHYSIAELFAVRGAIARLKPAVFHAPHYVVPVQPPAATVVTI
                     HDLMHLTRPEHGAPWKRAYAKWMVGRAMRLSARVIAVSEATREEILAFGPENGGKVVV
                     IPNGVREEFFEWGKRVAAARDEAAASEGQVPRPDSLSKEISSFLLFLGNDKPHKNLEG
                     LLRAWPHVRAEHPALSLVLAGVEPGPRELPEGAKAVGFVPDADVPALVALAEALVLPS
                     FAEGFGLPVLEAHAAGTPVACSDLPALHEAGGEAAVFFNPHDAATIANALNLLLGDEE
                     KRNLLRKKGRGRARQFSWDEVAARTADVYEDLLRRSSGAGTAGDAPTPFEGASHPHSF
                     EENRPPSSS"
     gene            complement(6217..7302)
                     /locus_tag="PLB01_06445"
     CDS             complement(6217..7302)
                     /locus_tag="PLB01_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQQ76976.1"
                     /translation="MRIAVDARPLEERPTGVGRYLEGLLTAWLDDRHGDDFVLLSPRP
                     VFVPPSLEGRVAVVPSAALPGTVWLQTAAGISARRAGADAFFGCLGIVPIAGGPPSVA
                     TVHDMTPLLFPEWHSWKNRLGFTPFIAGSVRAARRIAAVSEHSRRDLVERFPDAAAKT
                     TVVHNGVDVSAAPAQGPPPNEGRPYVLSLGTLEPRKNLPRLVEAMESIWDRRPAFPDL
                     VLAGTEGWGLPGLGERLRSSRHAARIRPAGYLAGGERSRWMAGARVFAYPSLYEGFGL
                     PPLEAMALGTPVVASSASSLPEVVGDAGLLPDPGDAAAIAAAIERAHDDEAFRGWAAA
                     KGPARAATFTWASAARKMRALFEEALS"
     gene            7353..7964
                     /locus_tag="PLB01_06450"
     CDS             7353..7964
                     /locus_tag="PLB01_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQQ76977.1"
                     /translation="MDLNLETAFDEPVDLSHEFEIPSGRLERPELLSLSPVSFTGRLE
                     KVDLGFVLAGRLSFSGVVACARCLAPVPFARKEEASWTFMPAHVKPVEKPRAARPADG
                     KPAKAAKAGKGAEKNEDDDGDELSAEDLDVLYYEDFVVPFDPLVEEQLQLELPMKALC
                     RDDCKGLCPQCGSDRNAAPCDCAPPSDERWKSLKTILETDEKN"
     gene            8014..8196
                     /gene="rpmF"
                     /locus_tag="PLB01_06455"
     CDS             8014..8196
                     /gene="rpmF"
                     /locus_tag="PLB01_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQQ76978.1"
                     /translation="MPNPKHRHSKTRRDLRRAHDFLKPKQTSNCPNCGVEKLPHRVCA
                     ACGWYKGREVTTGATK"
     gene            8198..9208
                     /gene="plsX"
                     /locus_tag="PLB01_06460"
     CDS             8198..9208
                     /gene="plsX"
                     /locus_tag="PLB01_06460"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQQ76979.1"
                     /translation="MTIAVDAMGGDHAPRVNVEGAVAAAIDFGLDTLLVGRRKEVESE
                     LLRTGYTGGRVGVVEADEVVLFDEPSITPIRKKRRASIRIAAECVRDGRASGMFTAGH
                     TGAAMVVAKMIMGVVEGIDRPALAAVLPGLKKKQVLLDVGANVNCRPEHYREFALMGH
                     FYAQEVLGVAKPKIGLMSVGEEEGKGTDTTREVFGLLKESGLNFVGNVEGRDVYSGDV
                     DVIVTDGFTGNVILKVSESLAHLVEQALKQEITRSLQASMGFLLSKDAFRSFKKRLDY
                     SEYGGAPLLGVKGACLIGHGRSNVKAVRNAIKAAHDFVQRRIPDKIRAKAITFAPPDV
                     TR"
     gene            9240..10223
                     /locus_tag="PLB01_06465"
     CDS             9240..10223
                     /locus_tag="PLB01_06465"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQQ76980.1"
                     /translation="MLHATIAGTGRAVPDRILSNADLEKMVETSDEWITSRTGIRERR
                     VVSEGDVLSDFCVKAATPALEAAGLAPMDLDTIILATCTPDHPIPGAAPVVQHKLGAK
                     KAAAFDLNAACSGWLYGLHVADGLVQAGKAKNILLIGAEFLTRFVDYTDRGTCVLFGD
                     GAGATVLKATASDHGVLSTAIRTDGEGSCFISMPGGGSTYPPNRPETLEAHLPFIQMK
                     GGDTFKAAIRSMVEVCKEVLAESGFTENDVAWLLPHQANERILTATADRLKIPAERCM
                     NNIARYGNTSAASIPIALDEYARDGRIKRGDLILITAFGGGLTWGAALVRW"
     gene            10228..11160
                     /gene="fabD"
                     /locus_tag="PLB01_06470"
     CDS             10228..11160
                     /gene="fabD"
                     /locus_tag="PLB01_06470"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQQ76981.1"
                     /translation="MNGVAFLFPGQGSQFAGMGKDLAERFPEAREVYARADAALAPLG
                     LVVSKVSWEGTDEDLKQTAVTQPAIVTHAVAVLAVLKAKGVVPTVAAGHSLGEYAALV
                     AAGALTLEDAVVLVRRRGTFMQEAVPAGRGAMSAVIGLAPELVVEACAEATAASGEVV
                     SAANFNSPEQTVIAGSAAGVAKAGEILKARGAKRVLPLPVSAPFHCALMKPAEQALSP
                     FLDATAFAPLAIPVVNNADADVETDGAAAREALRRQVCSPVRWVESVQRLSGMAGSAI
                     EVGPGTVLSGLAKRIAKEWPVKTTSTAAALDALA"
     gene            11163..11639
                     /locus_tag="PLB01_06475"
     CDS             11163..11639
                     /locus_tag="PLB01_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HQQ76982.1"
                     /translation="MAEKVTTNATRVLDAKKALYEVLVFAPEDFTAEEVSAKLGVPED
                     DVFKTLVAKGDRTGPLAAVIPGPARLSLKKLARVSGNRTVSMIPALDMEKLTGYVKGG
                     CSPFGMRKALPLYAHESAVGRARLVCSAGRRGVQVAMAGDDFLAVSGASPADLIED"
     gene            11655..12413
                     /gene="fabG"
                     /locus_tag="PLB01_06480"
     CDS             11655..12413
                     /gene="fabG"
                     /locus_tag="PLB01_06480"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HQQ76983.1"
                     /translation="MAVSFSLSGKIALVTGGSQGIGESIAKALAAAGAYVVVAARRED
                     KAAAVAQAIAAGGGSAEALRLDVADPASVTAGFKAVVDKHGKLDVLVNNAGITDDGLI
                     LRMPKDSWDRVIATDLTGVFLCAQEAAKAMLKKRVAGRIVNITSVVGLMGNPGQTNYA
                     AAKAGVVGFTKALAREIGSRGITVNAVAPGYIETSMTDALNEEQKKALVGQIVLGRLG
                     TGDDVAGAVVFLASDAAAYVTGTTLNVSGGLHIG"
     gene            complement(12467..13387)
                     /locus_tag="PLB01_06485"
     CDS             complement(12467..13387)
                     /locus_tag="PLB01_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76984.1"
                     /translation="MLDHSYDYRSNLEAARKVNWKIEDVIEGRTFDFGKPFLPEALAG
                     VEGIRCLSPREKLKLNQIRGFSYLYLFGLVEEYILPAVIEHAETRTHGDDYEMQALLH
                     FAEEEAKHIQLFKWFVQEFEKGFGTPCGGIGPAKEIAAAILNHSRLGVFLATLQIEWM
                     TQKHYLESVKDNAAEGLDPLFCSLLKHHWLEESQHAKLDTLIVDEIARKLDPLRIEAG
                     IDDYMSIGKMLDGGLTAQVQLDLESLQKAIGRTLSAAEKAEITGAQIKAYRWTFLASG
                     MTHPKFDKSLRELSARGHERVGDLARAVAA"
     gene            13501..14577
                     /locus_tag="PLB01_06490"
     CDS             13501..14577
                     /locus_tag="PLB01_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ76985.1"
                     /translation="MEKRSSQRIQFVRTPDGVQLAWAETGSGPLLVKASNWLSHLEYE
                     WESPVWRHWLRFFADHFRFVRYDERGCGMSDWNVDDLSCNRWLGDLEAVVDAALPEGA
                     FPLLGISQGVSTAVSYAVKHPERVSRLVLYGGYARGWALRGDSAGKREHEAIVDLAAL
                     GWGRDNPAFRQVFTSRFVPGATSEQMDWFNELCRRTSSPAIAADLLRARASVDVKELL
                     PKVSAPTLVLHAREDAAVPVSAGRQVAALIPGAQFVELDSKNHILLEGEPAWGRFCDA
                     VRDFMGIEHAARTDAGPFADLTARERGILGLLTEGLGNADIAERLGVSEKTVRNHVSN
                     LFDKLGVWTRAQAIVFARDRGFRG"
     gene            14749..14994
                     /gene="acpP"
                     /locus_tag="PLB01_06495"
     CDS             14749..14994
                     /gene="acpP"
                     /locus_tag="PLB01_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQQ76986.1"
                     /translation="MANSVEEKVKGIIVEQLGVQAEEVKPEASFVDDLGADSLDTVEL
                     VMAFEEAFGIEIPDEDAEKIQKVKDAVSYIESHAKPA"
     gene            15017..16288
                     /gene="fabF"
                     /locus_tag="PLB01_06500"
     CDS             15017..16288
                     /gene="fabF"
                     /locus_tag="PLB01_06500"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQQ76987.1"
                     /translation="MSEKHAPLLDARGRRRVAVTGVGLVSPIGLTRDENWASLKAGVS
                     GIGPLTRFDATEYACRIAGEVRGFDPGAFLDKKEIKKFDTFIHYAVAASKDAMTDSGL
                     VVNEENAERVGVCIGSGIGGLPLIEETHKTLLEKGPRRISPFFIPGLIVNMCSGLVSI
                     LNGAKGPNTATATACATSAHAIGDAVNIIRRGDADAMIAGGSESVVTPLAIGGFAAMR
                     ALSTRNDDPKGASRPWDKDRDGFVLGEGSGVLILEDLAHARARGATIYAELAGYGMSG
                     DAFHISAPSEDGDGPSRVMLNTLKDAGANPDEIAYINAHGTSTPHGDKIETTAIKKVF
                     GDHARKLAVSSTKSMTGHLLGAAGGYEAGVLALAMRHGVIPPTINYTTPDPDCDLDYT
                     PNTAREVRLRAALSNSFGFGGTNACLLMRSV"
     gene            16379..>18079
                     /locus_tag="PLB01_06505"
     CDS             16379..>18079
                     /locus_tag="PLB01_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQQ76988.1"
                     /translation="MAVAVAAGGFLWWRANRSFLDRWGEDASGLVSPARIGTQIEVLS
                     AKPHRAGSPANGEVGSEVERRLASAGLKPWSDVHDADVWEPVVLRLALAGPGGREIDL
                     HEREVPGDPATRAAKDEVPFLAYAPDADLEAPLVYANFGAAEDYETLRKAGVDPRGKV
                     AIVHVQGVCRGEKVAAAERAGVAALLVYLEPKDQGILKPAYPEGAGPHPAAASRGSLL
                     KYFRRPGDPRRAKEQGVDVLPKIPALAISAEAAEALLREMTGPAPPKDWKGTLAAPYA
                     LGPGKARVRVTVRGRTVRVKLRNVLASIPGADPKAPPVLVMGHYDAWVHGAVDPGSGA
                     AVVLETAEVLSRLAERGWRPARGVLFALWDGEEPGMFGSTAWVEAHLGSSGLPVAAAI
                     NIDSAARANDLFASLTPGLRGVFDEILPHVADPLSPGKTLADSAGQPALPGFSSDVAP
                     FLGFTAVPAVELGFGRWYGAYHTLYDTPAYASKIADPGSVRAAALAKAVVLFAGSLAT
                     PRIFPFRFGEVSAFAQRELREIQARYPGAMGWLPAALNPLDTHLSAFESAAAAWDVYA
                     R"
BASE COUNT         2765 a         6175 c         6476 g         2663 t
ORIGIN      
        1 ggcttacggc cgcgcagcgg ccgaacccgc cgccgccatc gtcaaggcgt acgtgcggga
       61 gtcgtttgga ggattttctg cttcggaaga agagaagaaa gaccttgggg agaagctgga
      121 gcgtttcagg tcagcgctgt ccagccgccc cgccgcgatt tcttagaagg gtgagtgggc
      181 ctttcgcagg tgcggggccg gtgcgattgg cggggccgcc cgccctcttc accttctaag
      241 aaaatcttcc tcttctcttt ctcttcttct tcctcttcgt cgccctggcg gacggctcag
      301 tagaagtagc cgttcacgtc gatgacgacg tgcgcggtgc cggccgagcc gttgaggatg
      361 cggaaggtcc cggagccgtc ggtcgcgagg ccgatgaccg tggcgtcggc tcgagtctgg
      421 ccggcccgga agctcagggt cgaggtgccg gggtatgccg cggtgccggg ataggcggcg
      481 aagaagccgg aggctgcggg ctgcgtgacg gtgacgttca tcgaaacccc gagcgcgccc
      541 ggtggaatgt tgcacgaggc cgtcgccttg aggagccgga cctccgaggg cgcgagtacg
      601 ggaccgccga gcgggcccgc gacgaggcgc gtgtcggcga gccggcaggg cgtgaccggg
      661 aagaagagga gcgagcttcc ggccgggagg acgggaatcg aggccgtcgc ggccgcgccg
      721 atgcataccg cggggtcggg attcgtctcg gtcacgccga ggccgactgg cgtgcccgcg
      781 gtcccggccg tgaaggtcac cgtgttcgtc ccctggcccg ccgtgatcgt tccgttcgtg
      841 atcgtccacg tgaacgtgct tcccgcgttc ggtcggaccg aggcgacgcg gccgggcgac
      901 cccgctccga cggtgggaga tgccgtgatc gcaagcggcg ggccgagtgg gcacgtgacc
      961 gtcagggcga cgggaacgtc gatctggggg gaaaaggtgt cgccgctctg gacgcagacg
     1021 tgcgcggcgt agtggcccgg tgcgagccct gtcgagttga acgtgacggg cacgtcctcg
     1081 ctcgcgagcg ggcccgtcga gccggaaggc aaacccgccg cgagccacgg gacaccggcc
     1141 gagccacagc ccgacgtcgg ggagacgggg agattccacc agagcggaaa cgccgccgta
     1201 tttccgatcg tcagcttggc gacgctctgt gtgcccgggg cctgcgtgga cgccagggag
     1261 ggcggcgtca cggcgatcac gggggcgccg ttcgcggtgt aatggggcgg gcacgtgacc
     1321 gtcgcgtagt ttgcgctctt caggcctgcc gaatcttcgg ctcgtacggt gaggctgtac
     1381 gagttgccga cctgcctctt gggtggggtg ccggtgtcgc cgggatcgtc cctcggaggt
     1441 ccgcacgcca cgcggaagcc gaggccggcg ttcgaggcgt cgatattcag gctcgtctgg
     1501 aagaagccct gcgcgcggaa gacgagcttg ttgtcgatga acgtgtgcag gttgaccatg
     1561 tagttcggcg aggccgacac ggacgtgtag gcgacgttga cgtagcagga atcctggcgc
     1621 gggtccggct gggtgcagta gggcgcctga ctgtcgatga aaccgatcag cggcgcgcgg
     1681 cccgaggcca gagcggtgcg cgtgttggac gcgtgggact cgaccaccat gccgccgtcc
     1741 gcagaagccg cgccttgatt cgcggcgggc tgcgctccgc gctcctccgg cgcacctgcg
     1801 gcgagggaaa gggggaggag cgccgcagcc aggattccga cgcgcatcga ttccggcatt
     1861 tattccgtct ttttctctgc gatggagcgc tacgggctgt agggagggca cgtgaccgtc
     1921 gcgtagttgg cgctcttgag ggtcgccgag tcctccgccc gcaccgtgag gctgtacgag
     1981 tttccgacct gccgcttcgg gacggccgtg tcggaaggat cgtccctcgg aggcccgcac
     2041 gcgacgcgga agccgaggcc ggcgttcgaa gcgctgacat acaggctcgt ctggaagaag
     2101 ccctgggcgc ggaagacgag cttgtcgtcg atgaacatgt gcaggtagac catgtagttc
     2161 ggcgcggccg agacggacgt gtaggcgacg ttgacgaagc aggcgtcctg ccgagggtcc
     2221 ggctgggtgc agtagggcgc ctggctgtcg atgaagctga tcaggggcgt gcggccggtt
     2281 gccagggcgg tgcgggtact cgagccgtgg gattcgacca ccatgccgcc gtccgggggt
     2341 ggcgcgacgg gtttcgcggc ggcctgcgcg gcccgctccc cgggaggcgc ggccgcgaga
     2401 caaaacggca gcagggcgaa cgtggcgagg gctcgcgtca ggctcatggt ctcctcctca
     2461 ctgaagcgag gagaagctta tcgcaacacg gtctcagtac gcgttcttgt gcgtgaagcc
     2521 ccaccggacg gtggcccaga ggatcttcac atcgaggccg acggaccagt tctcgatgta
     2581 gtacaggtcg tactcgatcc gcttgtcgat cggcgtgttg ccgcgccagc cgtggacctg
     2641 cgcccagccc gtgatccccg cgcgcacgcg gtggcggagc atgtactgcg ggaacttctc
     2701 cttgaactcc ttgacgaact ccggccgctc cggccgcggc cccacgagcg acatgtcgcc
     2761 cgtgaagacg ttcacgagct gcggcagctc gtcgagcgag gtcttgcgca ggaacgcgcc
     2821 cacgcgcgtc cgccgcgggt cgttctcctg cgtccactgg gcgccctcgt cctcggcgtc
     2881 cacgcgcatc gaacggaact tgatcatccg gaagaggcgc ccgtcgaggc ccatgcgctc
     2941 ctgcgcgtag aacacgggcc cgcggtcctc gagccagatc gcgagcgcga taaacgggaa
     3001 gagcaccgcg agcgcgacga ggccgaacgc cgagaggacg acgtccatgg tccgcttcac
     3061 gagcgagttc cagccctcga gcggcatctg cgtgaggttg atgacgggca gcccgtcgaa
     3121 gtcctcgacg ccggccttga acgtgacgta ctggaagagg tccggcacga cgcgcaggtc
     3181 gatcatctcg ttgccgacgt ccttcaggat cgcgagcatc gtgcggtgcg cctcgacggg
     3241 cagcgcgagg aacacggtgt cgacctcgtg cgcgcggatc aggtcgcgca cctcgcccgt
     3301 cgtcccgagc atgttcaagc cgcgatagtt ctcccgccgc ttggccgggt cgtcgtccgc
     3361 gaagccgacc ggcttcatgc cggtggcggg gtgctcgagg agcttgtccg cgagcgcgcg
     3421 gcccgagtgg cccgcgccga cgatcaggac gcggcgaacg ccggcgcccg tcgcccacat
     3481 cgtctcgagg tacttgcgga tcccgaagcg ggcgagcgag acgaggacga tgtcgaggac
     3541 gaggaacagg acgaggagct ggcggctcgt gtacgtgaag ccgcgccaga aggagaggat
     3601 gccgacgagg aggatcgtcc cgagcgtcgc cgcgacgagg atcgaaagga cctcgtcgat
     3661 cgcggagcgg tcgcgccgga tctggtacag gcggtggaag tagaagacga cgggccagac
     3721 gacgaggagg atcgggatca gcggcgtgta gttcccgaac ggctgcccgc ccttgggcgt
     3781 cggccacacg ctctcgaaac ggatcagcca cgccgcccag agggcgagca cggtcgcgag
     3841 cacgtccgcc gtgaggaaga cggcctgcag gcggcgggtc tgtctgcgga tcacacggtc
     3901 accgggggcg gcattttact tggggatgcg gccgggcgtg cgagcgcctc gtccagcgcg
     3961 cgccggaatc gcccgcggaa ggcttcccgc gagaaccgcg ccgcgttcgc gacggcggcg
     4021 gcagggtcga gcctcagcga ccgcaggcgg tcgaaggccg cgaggaggga ggcggcgccg
     4081 ggctcgggca tgaggacgcc cgtctccccg tcccgcaccg tctcgagcgc tccgccccgc
     4141 ccgagggcga tcacgggcgt gccgcaggcc tgcgcctcga ggggcacgat cccgaagtct
     4201 tcctcgcccg gcatgaggac agcctcggcc gaacggtaga gctggcggag catggtgtcg
     4261 ttgggagtgt cggcgaaaac caccgtctca ccggccatcg cctccagccg ccggctctcc
     4321 gggccgaagc cggcgatcgt gagggggagc ttccggacgt tggcggccgc gatcgcgtcg
     4381 tccagcctct tgtagggggc gagggccgag acgaccagga gcccgcgccg ctccccgccg
     4441 gggcccggcg tgtagaactc cgtgtcgacc ggcggcgaga cgaccgcggc gtcgcgccgc
     4501 cagaggcgcg cgatccggtc cttcacgttc gccgagttcg cgaggaacgc gtcgacgcgc
     4561 ggaaccgtcg ccacgtccca ggcccggagc ggggcgcgca cgagccgcgc cgcggccacg
     4621 accgccgcgg gccgcccgcg gaagtactcg tcgaactggt cgtgcaggta gcgcacgggc
     4681 gtgtggcagt aacagagatg gaaggctccg gcgtccttct tcgcgttctt cgcgacgcag
     4741 tgagacgtcg agatcacgag gtcgtacccc gagaggtccc acgactcggc cgcggcgaag
     4801 aagaggggga gcagcgggcg gtagttccgc gacggcgaca cgagcgactg gaggaacgtc
     4861 gtccggatct cccgcgcctc gatctcgggg tgctgcgagc ccgggaagtg aaagagcgtg
     4921 aagacgggcg cgtccgggaa catccggacg agctcgagca gcaccttctc gcctccgcgc
     4981 gtgcccgtca gccagtcgtg gacgagggcg actctcatga ggaagaggga ggacgattct
     5041 cttcgaatga gtgtgggtgc gatgcgccct cgaagggcgt cggggcgtct ccggccgttc
     5101 cggcgccact cgaccttcta agaagatcct catagacgtc cgcggttcgc gccgccactt
     5161 cgtcccacga gaactgcctt gcccgtcccc ttcctttctt tctaagcaaa tttctctttt
     5221 cttcatcccc gagcagcagg ttcagcgcgt tcgcgatcgt cgcggcgtcg tgcgggttga
     5281 agaagacggc cgcctctccc cccgcctcgt ggagcgccgg aaggtccgag caggcgacgg
     5341 gcgtgccggc cgcatgggcc tcgaggacgg ggagtccgaa gccctcggca aaggagggga
     5401 ggacgagcgc ctcggcgagc gccacgaggg cgggcacgtc ggcgtcgggc acgaacccga
     5461 ccgccttcgc gccctcgggc agctcgcgcg ggccgggttc gacccccgcg aggacgagcg
     5521 acagcgccgg gtgttctgcg cgcacgtgcg gccacgcgcg caggaggccc tcgaggttct
     5581 tgtgcggctt gtcgttgccc aggaaaagaa gaaaagaaga gatttctttt gaaagtgaat
     5641 cgggccgggg cacctgacct tcggacgccg ccgcctcgtc gcgcgcagcc gccaccctct
     5701 tcccccattc gaagaactct tctcttacgc cattcggaat tacgacgacc ttgccgccgt
     5761 tctcgggtcc gaaagcaaga atctcttccc gggtcgcctc cgaaaccgcg atcacgcgcg
     5821 cggacaggcg catcgcccgc ccgaccatcc acttcgcgta ggcgcgcttc cagggtgcgc
     5881 cgtgttcggg ccgcgtgagg tgcatgaggt cgtggatcgt gaccaccgtg gccgccggcg
     5941 gctggaccgg cacgacgtag tgcggcgcat ggaagacggc gggcttcagg cgcgcgatgg
     6001 cgccgcgcac ggcgaagagc tcggcgatcg agtagtggcg cgcgtcgcag gccacgggcg
     6061 aaacgcccgg cggcaggagc gcgtcgccgc ccgggtggac ggtcgcgcag aggtcccacc
     6121 gttcctgccg cacgaggccg ccgagcaggc cgcggatgta gctgccgatg ccgaagtccg
     6181 ccgccttgcg ggcgtccacg agaacgcggg gccgcgtcac gagagcgcct cctcgaagag
     6241 ggcgcgcatc ttccgcgcgg cggacgccca cgtgaacgtg gccgcccgcg ccggaccctt
     6301 cgcggcagcc cagccccgga acgcctcgtc gtcgtgggcg cgctcgatcg ccgcggcgat
     6361 cgcggcggcg tcgcccgggt cggggaggag gcccgcgtcg ccgaccacct cgggcaggga
     6421 cgaagccgaa gacgcgacga ccggcgtccc gagcgccatc gcctcgagcg gcggcaggcc
     6481 gaagccctcg tagaggctcg ggtacgcgaa gacgcgggcg cccgccatcc agcgggagcg
     6541 ttcgccgccc gcgaggtagc cggcgggccg gatgcgcgcg gcgtgccgcg aggagcgcag
     6601 gcgctccccg agaccgggaa ggccccagcc ttccgtcccg gcgaggacga ggtcggggaa
     6661 cgcgggccgg cggtcccaga tcgactccat cgcctcgacg aggcgcggga ggttcttgcg
     6721 cggttcgagc gtgccgagag acagcacgta gggccgcccc tcgttcgggg gcgggccttg
     6781 ggcaggcgcc gccgagacgt cgacgccgtt gtggacgacg gtggtcttcg cggccgcgtc
     6841 cgggaagcgt tcgacgaggt cgcgccgcga gtgttccgaa acggccgcga tcctcctcgc
     6901 ggcccggacg ctccccgcga tgaagggggt gaagccgagc cggttcttcc aggagtgcca
     6961 ctccgggaag aggagcggcg tcatgtcgtg gaccgtcgcg accgagggcg gcccgcccgc
     7021 gattgggacg atgccgaggc agccgaagaa cgcgtccgcg cccgcgcggc gcgccgagat
     7081 cccggcggcg gtctggagcc agaccgtccc ggggagggcc gcggagggga ccaccgccac
     7141 ccggccctcg agggagggag gcacgaagac cggacgcggc gacaggagga cgaaatcgtc
     7201 cccgtgccgg tcgtccagcc aggcggtcag gaggccttcg aggtagcgtc cgacgcccgt
     7261 cggcctctcc tccagcggcc gcgcgtcgac ggcgatgcgc atccggtcca tgttatattc
     7321 cgcggctcct cttcgctgag gagactccct gcatggacct gaatctggaa acggctttcg
     7381 acgagccggt cgatctttct catgaattcg agatcccgag cggacgtctc gagaggccgg
     7441 agcttctctc gctgtcgccg gtctcgttca cggggcgcct cgagaaggtc gacctcggct
     7501 tcgtcctggc cggacgcctc tcgttttcgg gcgtggtcgc ctgtgcgcgg tgcctcgcgc
     7561 ccgtgccgtt cgcgcggaag gaggaggcct cctggacctt catgcccgcg cacgtcaagc
     7621 cggtggagaa accccgggcg gccaggccgg ccgacggcaa gcccgccaag gccgcgaagg
     7681 ccggcaaggg cgccgagaag aacgaggacg acgacgggga cgagctctcg gcggaggacc
     7741 tcgacgtcct ctattacgag gacttcgtcg tgcccttcga ccctctcgtc gaggagcagc
     7801 tccagctcga gctgccgatg aaggcgctct gccgggacga ctgcaagggc ctctgcccgc
     7861 agtgcggctc cgaccgcaac gcggccccct gcgactgcgc cccgccatcc gacgagcggt
     7921 ggaagtcgct caagacgatc ctcgaaaccg acgaaaagaa ctgaagagaa cgaacggact
     7981 ttccgacctc gcaccgacca gaggagaccg acgatgccga acccaaagca ccgacacagc
     8041 aagacgcgcc gcgacctgcg ccgcgcgcac gacttcctga agcccaagca gacgtcgaac
     8101 tgcccgaact gcggcgtcga gaagctgccg caccgcgtgt gcgcggcctg cggctggtac
     8161 aagggccgcg aggtcacgac cggggccacg aagtagcttg acgatcgccg tcgacgcgat
     8221 ggggggcgac cacgcccccc gcgtcaacgt ggagggggcg gtcgccgcgg cgatcgactt
     8281 cggcctcgac acgctcctcg tgggacggag gaaggaggtc gagtccgagc tcctccgcac
     8341 gggttacacg ggcggccgcg tgggcgtcgt cgaggcggac gaggtcgtcc tcttcgacga
     8401 gccctcgatc accccgatcc gcaagaagcg gcgcgcctcc atccgcatcg ccgccgagtg
     8461 cgtccgcgac ggccgcgcga gcgggatgtt cacggcgggc cacacgggcg ctgcgatggt
     8521 cgtcgcgaag atgatcatgg gagtcgtcga ggggatcgac cggcccgcgc tcgccgccgt
     8581 cctcccgggc ctcaagaaga agcaggtcct cctcgacgtc ggcgcgaacg tgaactgccg
     8641 gcccgagcac taccgcgagt tcgcgctcat gggccatttc tacgcgcagg aggtcctcgg
     8701 cgtcgcgaag ccgaagatcg gcctcatgtc cgtgggcgag gaagagggca aggggacgga
     8761 cacgacgcgc gaggtcttcg gcctcctcaa ggagtccggc ctgaacttcg tgggcaatgt
     8821 cgagggacgc gacgtctact ccggcgacgt cgacgtcatc gtcacggacg gcttcaccgg
     8881 caacgtgatc ctgaaggtct ccgagagcct cgcgcacctc gtcgagcagg cgctcaagca
     8941 ggagatcacc cggagcctcc aggcgtcgat ggggttcctc ctctcgaagg acgcgttccg
     9001 ctccttcaag aagcggctcg actacagcga gtacggggga gcgccgcttc tcggcgtcaa
     9061 gggcgcgtgc ctcatcgggc acgggcgctc gaacgtcaag gcggtccgga acgcgatcaa
     9121 ggccgcgcac gatttcgtcc agcgacggat tcccgacaag atacgcgcca aggcgatcac
     9181 gttcgcgccg cccgacgtca cccggtaaga tccgcgcttt ccgccaggga ggagaaccga
     9241 tgctccacgc caccatcgcc ggcaccggcc gggccgttcc cgaccggatc ctctcgaacg
     9301 ccgatctcga gaagatggtc gagacgtcgg acgagtggat cacgtcgcgg accgggatcc
     9361 gcgagcgccg ggtggtcagc gagggcgacg tcctttcgga tttctgcgtg aaggccgcga
     9421 ccccggcgct tgaggcggcg ggcctggcgc cgatggacct cgacacgatc atcctcgcga
     9481 cctgcacgcc cgatcacccg atcccgggcg ccgcgcccgt cgtccagcac aagctcgggg
     9541 ccaagaaggc ggcggcattc gacctcaacg cggcctgctc ggggtggctc tacgggctcc
     9601 acgtcgccga cggcctcgtg caggcaggga aggcgaagaa catcctcctg atcggcgccg
     9661 agttcctgac gcgtttcgtg gactacaccg accgcggcac gtgcgtcctg ttcggcgacg
     9721 gggcgggcgc cacggtcctc aaggcgacgg cgtccgacca cggcgtcctc tcgacggcga
     9781 tccggacgga cggagagggc tcgtgtttca tctcgatgcc cggcggaggg tcgacctatc
     9841 ccccgaaccg gcccgagacg ctcgaggcgc acctcccgtt catccagatg aagggcgggg
     9901 acacgttcaa ggccgcgatc cgctcgatgg tcgaggtctg caaggaagtc ctcgccgaga
     9961 gcgggttcac ggagaacgac gtcgcatggc tgctcccgca tcaggcgaac gaacgcatcc
    10021 tcacggcgac ggccgaccgc ctcaagatcc ccgccgagcg gtgcatgaac aacatcgcgc
    10081 ggtacggaaa cacgagcgcg gcgtcgattc cgatcgccct cgacgagtac gcgcgcgacg
    10141 gccggatcaa gcgcggcgac ctcatcctca tcacggcgtt cggcggcggg ctgacctggg
    10201 gcgcggccct cgtccgctgg tgacgccgtg aacggcgtcg cgttcctttt ccccggccag
    10261 ggctcgcagt tcgcggggat gggcaaggac ctcgccgagc gcttcccgga agcgcgcgag
    10321 gtttacgcgc gggcggacgc cgcgctcgcg ccgctcggcc tcgtcgtctc gaaggtgtcg
    10381 tgggaaggca ccgacgagga cctcaagcag accgccgtta cgcagccggc gatcgtcacc
    10441 cacgccgtcg ccgtcctcgc ggtcctcaag gcgaagggcg tcgttcccac ggtcgcggcg
    10501 gggcactcgc tcggagagta cgcggcgctc gtggccgcgg gcgcgctcac gctcgaggac
    10561 gccgtcgtcc tcgtgcggcg gcgcggcacg ttcatgcagg aggccgtccc ggccggccgc
    10621 ggcgcgatgt cggccgtcat cggcctcgcc cccgagctcg tcgtggaggc ctgcgcggag
    10681 gcgaccgcgg cctcgggaga ggtcgtctcg gccgcgaact tcaactcgcc cgagcagacg
    10741 gtcatcgcgg gctcggccgc gggcgtcgcg aaggccgggg agatcctcaa ggcgaggggc
    10801 gccaagcgtg tcctcccgtt gccggtctcg gcgccgttcc actgcgcgct catgaagccc
    10861 gccgagcagg cgctctcgcc gttcctcgac gccacggcct tcgccccgct cgcgatcccc
    10921 gtcgtgaaca acgccgacgc ggacgtcgag acggacggcg ccgccgcacg cgaggccctc
    10981 cgccgccagg tgtgctcgcc cgtgcggtgg gtcgagtccg tccagcgcct ttccgggatg
    11041 gccggctccg cgatcgaggt cgggccgggg acggtcctct cgggcctcgc gaagcgcatc
    11101 gcgaaggagt ggcccgtgaa gacgacgtcc accgccgcgg cgctcgacgc cctggcctga
    11161 acgtggccga gaaggtcacg acgaacgcga cccgcgtcct cgacgcgaag aaggccctct
    11221 acgaggtcct cgtcttcgcg cccgaggact tcacggcgga ggaggtctcc gccaagctcg
    11281 gagttccgga ggacgacgtc ttcaagacgc tcgtcgcgaa gggcgaccgg acggggcctc
    11341 tcgccgcggt gatcccgggt ccggcccgcc tctctctgaa gaagctcgcc cgcgtctcgg
    11401 gcaacaggac ggtctcgatg atccccgccc tggacatgga gaagctcacg ggctacgtga
    11461 agggcggctg ctcgccgttc gggatgagga aagccctgcc gctctatgcc cacgagagcg
    11521 cggtcggacg cgcgaggctc gtctgctccg cggggcggcg gggcgtgcag gtcgcgatgg
    11581 ccggggacga cttcctcgcc gtttcgggcg caagtcctgc cgatctgata gaagattgac
    11641 cgaaaggaac gccgatggcc gtttcgtttt ccctttccgg aaagatcgcg ctcgtgaccg
    11701 gcggctccca gggcatcggg gagtcgatcg cgaaggcgct cgccgccgcc ggggcgtacg
    11761 tcgtcgtggc cgcgcgccgc gaggacaagg ccgcggccgt cgcgcaggca atcgccgcgg
    11821 ggggcggctc ggccgaggcg ctccggctgg acgtcgccga ccccgcctcg gtgacggcgg
    11881 gtttcaaggc cgtcgtcgac aagcacggaa agctcgacgt cctcgtgaac aacgcgggga
    11941 tcacggacga cggactcatc ctgcgcatgc cgaaggactc gtgggaccgc gtcatcgcga
    12001 ccgacctgac cggcgtcttt ctgtgcgcgc aggaggccgc gaaggccatg ctcaagaagc
    12061 gggtcgcggg ccgcatcgtg aacatcacgt ccgtcgtggg cctcatgggg aatcccggcc
    12121 agacgaacta cgcggccgcg aaagccggcg tcgtgggctt cacgaaagcc ctcgcgcgcg
    12181 agatcggctc gcgcgggatc accgtcaacg cggtcgcgcc cggatacatc gagacgtcca
    12241 tgacggacgc cctgaacgag gagcagaaga aggccctcgt cggccagatc gtcctcgggc
    12301 gtctcgggac gggcgacgac gtggccggcg ccgtggtctt cctcgcctcg gacgcggccg
    12361 cctacgtgac ggggacgacg ctcaacgtga gcggcggcct gcacatcgga taggccgcga
    12421 acgagagggg agcgcctcgc cgaggtcgct ccccgtcccg gctccgtcag gcggcgacgg
    12481 cccgcgcgag atctccgacc cgctcgtgcc cccgggcgct cagctcccgc aggctcttgt
    12541 cgaacttcgg gtgcgtcatg ccgctcgcga ggaacgtcca gcgatacgct ttgatctggg
    12601 ccccggtgat ctcggccttc tccgcggccg agagcgtgcg tccgatcgcc ttctgcaggc
    12661 tctcgaggtc gagctggacc tgcgcggtca ggccgccgtc gagcatcttg ccgatcgaca
    12721 tgtagtcgtc gatccccgcc tcgatcctca gcggatcgag cttgcgcgcg atctcgtcga
    12781 cgatgagcgt gtcgagcttg gcgtgctggc tctcctcgag ccagtggtgc ttgaggaggc
    12841 tgcagaagag cggatcgagc ccttccgccg cgttgtcctt cacgctctcg aggtagtgtt
    12901 tctgcgtcat ccactcgatc tggagcgtcg cgaggaagac gccgaggcgc gagtggttga
    12961 ggatcgcggc ggcgatctcc ttcgccggcc cgatgcctcc gcagggcgtg ccgaagccct
    13021 tctcgaactc ctgcacgaac cacttgaaga gctggatgtg cttggcctcc tcctcggcga
    13081 agtgcaggag cgcctgcatc tcgtagtcgt cgccgtgggt gcgggtctcg gcgtgctcga
    13141 tgaccgcggg aaggatgtac tcctcgacga gaccgaagag atagaggtag ctgaagccgc
    13201 ggatctggtt cagcttgagc ttctcccggg ggctcaggca gcggatgccc tcgacgcccg
    13261 cgagggcctc cggcaggaac ggctttccga agtcgaaggt ccgtccctcg atgacgtcct
    13321 cgatcttcca gttgaccttc cgcgcggcct cgaggttcga acggtagtcg tagctgtggt
    13381 ccagcatggt gtgtcctcct gggcctcaag atggcctcga tgggccgccg ctcccatggg
    13441 aacaatgtcc cgatctggct atgatcgcgg ccgatcgccg ggacgaatgt cccgttggcc
    13501 atggaaaaga ggtcgagcca gcgcatccag ttcgtccgca cgccggacgg agtccagctc
    13561 gcctgggccg agaccgggtc gggtccgctg ctcgtcaagg cctcgaactg gctgagccac
    13621 ctcgagtacg agtgggagag tcccgtctgg cggcactggc tccgcttctt cgcggaccac
    13681 ttccggttcg ttcggtacga cgagcgcggc tgcggaatga gcgactggaa cgtcgacgac
    13741 ctctcgtgca accgctggct cggcgatctc gaggccgtcg tcgacgccgc cctccccgag
    13801 ggcgccttcc cgctcctcgg gatctcgcag ggcgtgtcga cggccgtctc ctacgcggtg
    13861 aagcacccgg agcgcgtctc gcgcctcgtc ctgtacggcg gctatgcgcg cggctgggcc
    13921 ctgcgcgggg actccgccgg aaagcgcgag cacgaggcga tcgtcgacct cgccgctctc
    13981 ggctggggcc gggacaatcc cgcgttccgc caggtgttca cctcgcgttt cgttccggga
    14041 gcgaccagcg agcagatgga ctggttcaac gagctctgca ggaggaccag ctcgccggcg
    14101 atcgcggcgg acctcctgcg cgcgcgcgcc agcgtcgacg tcaaggagct cctcccgaag
    14161 gtgagcgccc cgacgctggt cctccacgcg cgcgaggacg ccgccgtgcc ggtctccgcc
    14221 gggcgccagg tcgcggccct catccccggc gcccagttcg tcgagctgga ctcgaagaac
    14281 cacattcttc tcgagggcga gccggcctgg gggcgtttct gcgacgccgt gcgcgacttc
    14341 atggggatcg agcacgcggc gcggacggac gcgggcccct tcgcggacct cacggcgcgc
    14401 gagcggggca tcctcgggct cctcaccgag gggctcggaa acgcggacat cgcggaacgc
    14461 ctcggcgtga gcgaaaagac cgtccgcaac cacgtgtcca atctcttcga caagctcgga
    14521 gtctggacgc gcgcccaggc gatcgtcttc gcgcgcgacc gcggttttcg cggctgaatc
    14581 cgcctttttc gagcagtttc gcgcggctgc gccgatcggc gacagtcggt tctgcccccg
    14641 gaattcggcg ttccgctcct tcggttagac tccgcaagct tgaaatcgtg acccggcagc
    14701 gtacagaatg ccggtctctc acacgaagaa gattttcgga ggatcgcgat ggcgaattcg
    14761 gtggaagaaa aggtcaaggg catcatcgtc gagcagctcg gcgtgcaggc cgaggaagtg
    14821 aagcccgagg cgtcgttcgt ggacgacctc ggcgcggact cgctcgacac ggtcgagctc
    14881 gtcatggcct tcgaggaagc gttcggcatc gagatcccgg acgaggacgc cgagaagatc
    14941 cagaaggtca aggacgcggt ctcctacatc gagtcccacg ccaagccggc ctgaggcggc
    15001 gcggactcaa cccttcgtgt cggaaaagca cgctcctctc ctcgacgccc gcgggcggcg
    15061 ccgcgtggcg gtcacgggcg tcggcctcgt gtcgccgatc ggcctgacgc gggacgagaa
    15121 ctgggcgagc ctcaaggccg gggtctccgg catcggcccg ctgacccggt tcgacgccac
    15181 ggaatacgcg tgccggatcg cgggcgaggt gcgcggcttc gaccccggag ccttcctcga
    15241 caagaaggag atcaagaagt tcgacacgtt catccactac gcggtggcgg cgtcgaagga
    15301 cgcgatgacc gactccggcc tcgtcgtgaa cgaggagaac gccgagcgtg tcggcgtctg
    15361 catcggttcg ggtatcggcg ggctgccgct catcgaggag acccacaaga cgctcctcga
    15421 gaaggggccg cgtcgcatca gcccgttctt catccccggg ctcatcgtga acatgtgctc
    15481 ggggctcgtc tcgatcctga acggcgccaa ggggccgaac acggcgacgg cgacggcctg
    15541 cgcgacgtcg gcgcacgcga tcggcgacgc cgtgaacatc atccgccgcg gcgacgcgga
    15601 cgcgatgatc gcgggcggct ccgagtccgt cgtcacgccg ctcgcgatcg gcgggttcgc
    15661 cgcgatgcgc gctctctcga cgcgcaacga cgacccgaag ggcgcctcgc gcccctggga
    15721 caaggaccgc gacggtttcg tcctcggaga gggatcgggc gtcctgatcc tcgaggacct
    15781 cgcgcacgcg agggctcgcg gcgcgacgat ctacgcggag ctggccggct acgggatgtc
    15841 aggcgacgcg ttccacatca gcgccccgtc ggaagacggc gacggcccct cgcgcgtcat
    15901 gctcaacacg ctcaaggacg ccggcgcgaa tcccgatgag atcgcgtaca tcaacgccca
    15961 cggcacctcc acgccgcacg gcgacaagat cgagacgacg gcgatcaaga aggtcttcgg
    16021 cgaccacgcc cggaagctcg cggtctcgtc cacgaagtcc atgacgggtc accttctcgg
    16081 ggcggccggc ggatacgagg ccggcgtcct cgcgctcgcg atgcgtcacg gcgtcatccc
    16141 gcccacgatc aactacacga cgccggaccc cgactgcgac ctcgactaca cgccgaacac
    16201 ggcgcgcgag gtgcgcctcc gcgcggcgct ctcgaattcg ttcgggttcg gcggcacgaa
    16261 cgcgtgcctc ctgatgcgct ccgtctgacg cgcgcgctcg cgggggccga ccggcctgct
    16321 acgataaacc gtgtttccct tcgggcgttc gcgtcgtgcg gccgcggttg cggccctggt
    16381 ggcggtcgcc gtggccgcgg gcggctttct ctggtggcgc gcgaaccggt ccttcctcga
    16441 ccgatggggt gaggacgcct ccggcctcgt ctcgccggcg cgcatcggga cgcaaatcga
    16501 ggtgctctcc gcgaagccgc accgcgcggg ctcgccggcg aacggcgagg tgggttccga
    16561 ggtcgagcgc cgcctcgcgt cggccgggct gaaaccgtgg agcgacgtgc acgacgcgga
    16621 cgtctgggag cccgtcgtcc tgcgcctcgc gctcgccggc cccggcgggc gcgagatcga
    16681 cctccacgag cgcgaggtac cgggcgatcc cgcgacgcgc gccgccaagg acgaggttcc
    16741 gttcctcgcc tacgctcccg acgcggacct cgaggcgccg ctcgtctacg cgaacttcgg
    16801 tgcggccgag gactacgaaa cgctccgcaa ggcgggggtc gacccgcgcg gcaaggtcgc
    16861 gatcgtccac gtccagggag tctgccgcgg cgagaaggtc gccgccgccg agcgtgccgg
    16921 cgtcgccgcg ctcctcgtgt acctcgagcc gaaggaccag gggatcctga agccggcgta
    16981 tccggagggc gccgggccgc acccggccgc ggcctcgcgc ggctcgctcc tgaagtactt
    17041 ccggcgcccc ggcgacccgc ggcgggcgaa ggagcagggc gtcgacgtcc tgccgaagat
    17101 tccggcgctc gcgatctccg cggaggcggc cgaggcgctc ctcagggaga tgacggggcc
    17161 cgccccgccg aaggactgga aggggacgct cgccgcgccg tacgcgctcg gacccggcaa
    17221 ggcgcgcgtc agggtcaccg tgcggggccg gacggtccgc gtgaagctgc gcaacgtcct
    17281 cgcgtcgatc cccggcgccg acccgaaggc gccgccggtg ctcgtgatgg ggcactacga
    17341 cgcctgggtg cacggcgccg tcgaccccgg ctccggcgcg gccgtcgtcc tcgagacggc
    17401 cgaggtgctc tcgcgcctcg cggaaagagg ctggcggccc gcgcgaggcg ttctcttcgc
    17461 gctctgggac ggcgaggagc cggggatgtt cggctcgacc gcctgggtcg aggcgcacct
    17521 cggctcgtcc ggacttcccg tcgccgcggc gatcaacatc gactcggccg cgcgcgcgaa
    17581 cgacctcttc gcgagcctca cgcccgggct gagaggcgtc ttcgacgaga tcctgccgca
    17641 cgtggccgac cccctctcgc ccgggaagac gctcgccgac tcggcggggc agcccgcgct
    17701 tcccggcttc tcgagcgacg tggcgccgtt cctcgggttc acggccgtgc cggccgtcga
    17761 gctcgggttc gggcgctggt acggcgcgta ccacacgctc tacgacacgc cggcgtacgc
    17821 ctcgaagatc gcggacccgg gctccgtccg cgccgcggcg ctcgcgaaag ccgtcgtact
    17881 cttcgccggc tcgctcgcga cgccgcgaat cttcccgttc cggttcggcg aggtgtccgc
    17941 gttcgcgcag cgcgagctgc gcgagatcca ggcgcgctac cccggcgcga tgggctggct
    18001 gcccgccgcg ctcaatcctc tcgacacgca cctctcggcc ttcgagtcgg ccgcggccgc
    18061 gtgggacgtt tacgcgcgg
//
LOCUS       DAOMFY010000053        18047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_971_length_18047_cov_7.729491, whole genome shotgun sequence.
ACCESSION   DAOMFY010000053 DAOMFY010000000
VERSION     DAOMFY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18047
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_18047_cov_7.729491"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..2034
                     /locus_tag="PLB01_06510"
     CDS             235..2034
                     /locus_tag="PLB01_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ76989.1"
                     /translation="MAPGESTETAFRKQVRGQWFGWLAAQEEGDTALARRKVDEILKH
                     AQKIEIRRMTDLALSATLLGRRQLVAGKPTLAREAFAAAIRLDPDLPEPRWARLGLAF
                     RAREPGEIVSGLAGALRATVADGESRRVNLARAVLLVLGTVAAATLAALVILLVRHGG
                     RLVHDLKETAGGFLRGRATVALVLGVLVLPLLLGLDVVWLSLFLFVLVFGYASLDQKI
                     ALSGALVVLVALFPALDRFAYEFAISSSPILRGAEALQEARYDQRVLDNLEAAKNIAP
                     DDVDIRFLLGRLYQSLGQNDRATFEYTAGAQLAPRDTRCLVNRGNIRFVDGDLGSSQE
                     DYQQALKRDPRSIAARYNLALLYAETFRTVEAAQTLQEARALDAPAVQRFQDTPTLVK
                     VVSIGYSTDEARRKIEEMERVGRSRRLLGHFRSWDPFGGLRVPLVVAFLVAVAAAWAL
                     DSFRKRGRGYASECQKCGRAFCRLCKPSAESALLCSQCIHVYLKKDGVAIETKLQKLD
                     DVRRRKSFEERLRGILNAVFPGAAAFLDSRLPLASLAFGLFAFGLLAAFWGPRLAVLP
                     RPTLSGGVLAVAFWCAVALAGWLVGAFTSPRKA"
     gene            2037..3344
                     /locus_tag="PLB01_06515"
     CDS             2037..3344
                     /locus_tag="PLB01_06515"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HQQ76990.1"
                     /translation="MALEGTLRDFSLADILQLIALQHKTGLLSVRSPGDTVTLGFVDG
                     QLVAAESSAQRLDTRLGTVLVKTRRLSPEALQRALEMQAQTLQRLGFILLRNGFCSAQ
                     DLSDGLDIQIRRIVYGLFRWTDGDYVFEQTDHVDYDPESTTPISVERLLMEGARMLDE
                     WPIVEKVVWSLDLVYQKVPVAQPVIPAEDEDEVEVVEDSTVARRARDRRPEPIRVSRA
                     EWSVYELVDGQRTVAEINELAFLSEFDGCKAFFDLVSRGLVEEAMPRAVEAPDEAGAT
                     VGVPAARVPRAPLDLNVIAGVGLALVLAFAVRMQSKNPLNLVTAPPRRDAVVGGYEKS
                     LSLLKLRRLAEGVDTYTLISGRLPDGLDSLVASQILRMRDLEDPWGTKYRYIVQGEKF
                     YLVGFDADGHTDPDLLFSRTLVLRETKEQELAPHSTREITVVE"
     gene            3409..5367
                     /gene="dnaK"
                     /locus_tag="PLB01_06520"
     CDS             3409..5367
                     /gene="dnaK"
                     /locus_tag="PLB01_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQQ76991.1"
                     /translation="MLSYPSARRASQRSPGQMSKIIGIDLGTTNSVVAVLEGGTAEVV
                     ANSEGGRTTPSVVGYAKTGERLVGQVAKRQSVTNPENTVYSIKRFMGRRFDEVNDEMK
                     MVPYKVVRSSNGDARVLAGGKELSPPEISAMILTKLKEAAEAYLGEKVDRAVITVPAY
                     FNDAQRQATKDAGQIAGLKVERLVNEPTAAALAYGLDKKKNELIAVYDFGGGTFDISI
                     LEVGDGVVEVKSTNGDTHLGGDNIDQRIVEWLEAEFKKDQGIDLSKDPMAVQRLKEAA
                     EKAKIELSTTVETEVNLPFITADASGPKHLTLKLTRAKLESLVDDILQRSVGPCKQAL
                     KDAGVDASKIDEIVLVGGQTRMPKIQQIVKDFFGKEPNKSVNPDEVVAIGAAIQGGVL
                     AGDVKDLLLLDVTPLSLGVETLGGVMTKLIERNTTIPAKKSEVFSTASDGQTSVEIKV
                     LQGEREMAGDNKLLGVFSLIGIPPAPRGLPQIEVTFDIDANGILNVSAKDRGTGKEQK
                     ITITSSSGLDKNEVEKLVKDAQAHSGEDKKRREAIEAKNGLDALVYQTEKILEESGDK
                     VPAAEKDAVTAAVADAKKVLESQSGDAEALKKAAQELQKASYKIAEALYKQTPPPAGG
                     ASEGGAPGGPAGAGKEDVIDAEVVEEKK"
     gene            5374..5760
                     /locus_tag="PLB01_06525"
     CDS             5374..5760
                     /locus_tag="PLB01_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQQ76992.1"
                     /translation="MDREEGYVFIGVVAKRFGVHPQTLRLYEREGLIRPARSSGNTRL
                     YDADTVERLEIILTLTRELGVNLAGVEVILDLKSRLARMEGEVGRLMEAIRAVAKDHA
                     ARAGAGPKDDKALAVRPRGAIVLKYD"
     gene            5803..6690
                     /locus_tag="PLB01_06530"
     CDS             5803..6690
                     /locus_tag="PLB01_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HQQ76993.1"
                     /translation="MTSRLRPDDDSKVLRKYLDDISRYPILAAERERELGRIIQDLST
                     PEEARKEAVDELVRGNLRFVVSYAKRFHSPEVSFLDLINEGNIGLIQAARRFDPERGV
                     KFITYAVWWVRQAISNALSEQWGAIRLPHKQATLHSRLGRVKEALTRALGREPTMDEI
                     AAEAGLKVIDAENLMGMTRSSESLSGVFGLEEDRTLGDTLEQTSVAAADEQLLRRSSI
                     DQTRTLLDALPKKERAILCRRFGIPEDGTEGEREPMTLQEIGEELHLSRERVRQIEAQ
                     AIARIKRSLKSRALKAFLN"
     gene            6690..7412
                     /locus_tag="PLB01_06535"
     CDS             6690..7412
                     /locus_tag="PLB01_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ76994.1"
                     /translation="MSTVAAPEPCVLCKGFGVVETPEGRVVACRCRLGDLAEARLRAA
                     QIPERYRNCTLENYIVHKDAPPSQRVAKMKSVKFVEEWKSVRYGLLFIGTVGVGKTHL
                     ATAILRRLIEECGAKGVFCDFSDLLERIKATFSKTNPDSADDVLAPYREAEILVLDEL
                     GAQRPTDWVRDVLYGLINTRYNRQRITIVTSNYGDAPRTAGEETLESRVGVLVRSRLH
                     EMCVPVLIEGDDYRKKQRAWSA"
     gene            7405..8403
                     /locus_tag="PLB01_06540"
     CDS             7405..8403
                     /locus_tag="PLB01_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ76995.1"
                     /translation="MPETGAPAPRTRSAPAAFGRGVSQVVANPGLLLAPLAFGGIVAA
                     SIVAAAFVAAFWFGRAFASAARMAERGPGALSDLLEGLRGLLFAAPGAVVLALLAVLV
                     GLLLLTALAAWIRAGVTGSLAEIDRRAGEGAPLSAFRHPMLGPLFFGSAGRLFGGFFA
                     LVNLYALVASFAVLLIVLPLVGAFVAVEAGRSGLVVLFALLFVVLALVGIVASAALRV
                     VYLVAGRVLAIEGLDALAAAGRAIALVRASPGRAATLYLLTVGGAMAVGLAFVFPRIV
                     LTFAAGWTDAGAWAPVLVSGAFILLQMGAGFAYDLSVTGAFVALWPADEAKEPPLP"
     gene            8400..10340
                     /locus_tag="PLB01_06545"
     CDS             8400..10340
                     /locus_tag="PLB01_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HQQ76996.1"
                     /translation="MKRLALSAVLAIVAALGGCASPSAPVVAPLAATPAVPPTPVPSP
                     VPTPASAPRLLPLPSPALNVSLAVDRSSAAFPAGDWVLAAGERVERRRGPLVVAPANG
                     GLRVTDDGVSSDWPSPVEIAPANGALVPWADATYRGRLALRATVRGTLHVINRVGLED
                     YLKGVVPAEMGPRVYDEVEALKAQAVAARSYAAKHRGQFAAEGYDLCATPRCQVYSGA
                     SAENPLSTRAVEETAGEVLLWKGAVADALFTSTCGGRTENAPDVMAGYAKVDAPYLAS
                     VACFGETPVLLESSAVAKKGTVTLLGARGRALAASFEEGEGAGEGAKIPQKAREALQR
                     RLGVAAHGASRPHPLSVPAVYLEIASAAGFDTAVLSEESDRGELPSNWSEKAKASFAA
                     LQRFQLGGGSALPTDRALKPEEAAGIYAVLLSRMGDFEDVEGRITKSDDTGIEVKGAK
                     GRSSYSLSKKSSVLFEGGNEALIQVASHRFFPGDRVRVFVRDGKALGVVFPPAPAAGA
                     YDRESAWEHWTRRFTAKDLGAKIREREGTRTAADPAGIAVTSRGVSGRARTVAVATAK
                     GTVTLTGLEIRFALGLPESLFNVVAGRDEDGERVFTFYGRGWGHGVGLCQTGAFGMAL
                     SGRTYREILAHYYPGAEVAQVP"
     gene            10378..10965
                     /gene="amrA"
                     /locus_tag="PLB01_06550"
     CDS             10378..10965
                     /gene="amrA"
                     /locus_tag="PLB01_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQQ76997.1"
                     /translation="MRRDDGLRLSPEQRRDLLVRARGAAARALGIPFAGILPSLPSGR
                     LAEPGMCFVTWKRDGQLRGCIGSVEPVRPLWADVEANAVHGLLEDPRFPPATPDELAH
                     LRLEISVLTLFEPVADPLRDVRIGVHGLLVERGPHRGLLLPQVPVEWGWDVPAFLAHS
                     CRKAGLPADAWRDPRTTVSTFEAEVFGEQDGAAGG"
     gene            10974..12227
                     /locus_tag="PLB01_06555"
     CDS             10974..12227
                     /locus_tag="PLB01_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HQQ76998.1"
                     /translation="MPSAATSADVASVVSIRPHFPALERREGGAPVAFFDGPGGTQVP
                     RAVADAMTDYLLRHNANTHWVYPTSVETDAMITASRAALADFLGGDPSEIVFGANMTT
                     LTFHLSRALARGWQPGDEVVVTELDHHANVDPWRALERDFGVVVKAAPLVPGEARLDL
                     GALGRLLTKKTRLVAVGAASNAVGTINDVARVAALARANGSLAFVDAVHYAPHALVDV
                     KALGADFLACSPYKFYGPHAGVLWGKRALLEALDAPKLRPAPENAPDRLETGTQNHEG
                     IAGCGAAVDFLASLAGGGGDRRSRLARTFAALHARGEELLSRMWNGLSQVPGVTLYGP
                     PPGAPRTPTVGFTVEGWDSAAVARALVPHGVYVSNGDFYATTLVEKLGHGADGLVRAG
                     AACYTTPDEVDRLVSAVARLSPRGH"
     gene            12289..13482
                     /locus_tag="PLB01_06560"
     CDS             12289..13482
                     /locus_tag="PLB01_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQQ76999.1"
                     /translation="MAAPVASAPPVTGFGFALTEEQEAVRAMVRDFARTEIAPHVMEW
                     DEAQTFPRGVMAKLGELGMLGVLVPEEYGGAGLSYLDYIHVVDEISRVDGSIGLGVAA
                     HNSLCTNHLWLFGSEAQRRRWVTPLAQGKLIGAWGLTEAEAGSDSGGAKTTAVKDGDS
                     WVLNGSKNFITHASVGDVAVLLAVTDKAAGRNRISAFVVDLHQKGIHAGKKENKLGMR
                     ASDTATLVMEDCRIPAENLIGAPGEGFVQAMKVLDGGRISIAALALGIAEGAFEGARA
                     YAQQRKQFGKAISEFQTIQNYLADMATEIDAARLLIYRAGWMKDAGRMTTKESAMAKL
                     YASEVSVRVSNLAVQIHGGYGFTKDFGAEKYFRDAKLCTIGEGTSEIQRLVIAREILK
                     KAAGA"
     gene            complement(13479..13649)
                     /locus_tag="PLB01_06565"
     CDS             complement(13479..13649)
                     /locus_tag="PLB01_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77000.1"
                     /translation="MLERGAAGGYQDYQLRKSREVQSLKVESLRNPLKNVRTIESPLF
                     VMKEGRAVLDRR"
     gene            13763..14275
                     /locus_tag="PLB01_06570"
     CDS             13763..14275
                     /locus_tag="PLB01_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77001.1"
                     /translation="MRVSALAIAVLLVSGTKGSSAGPEPPRPSFAARAVPKGALAQAV
                     TPASSLAAERLGTSACRGIFEELPDFTGRPVARRLEAGERSPSSHFARLTFLGSTEGP
                     CRYSGTAAWSVAGDTRVRVCARTFGAVAAHDRREAAAVLIHEALHTLGVGEDAAKEPL
                     TAFVRRRCGL"
     gene            complement(14279..16222)
                     /locus_tag="PLB01_06575"
     CDS             complement(14279..16222)
                     /locus_tag="PLB01_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQQ77002.1"
                     /translation="MAALEHRTVCNRDCPDACGLIATVEDGKLVALGGDPSHPVTKGF
                     LCYRTSRFPEMQNGPLRLTTPLIRKTGVLAPATWEEALGFVADRLLAIRKESGPAAIL
                     HYRSGGSLGVVKSVVDLFFEALGPVSGKRGDICSGAGDAAQVIDFGREEAHDIFDVAN
                     ARNVILWGKNPAVSNVHLQPILREAKRKGARILLVDPIRHKSEKLADRTILPRPGGDF
                     DLAMGVAARLFETGGIDSAAPSYCDHFDAFRALALSRTAAAWAGAAGVAPDDVDALAA
                     ALAARPCSIQVGWGMGRRMNGAGIVRALDALGAVTGNLGIPGGGVFFSAVRRAPFDFS
                     FLSKSHPRTFPEPLLGEAILAAQDPPIRAVWVTAGNPVAMLPDSATVARALETRELVV
                     VVDPFLTDTARRATVVFPTTTLVEDDDLLGSYGHHWIGASTPVVAPPAGVLTDLEIVQ
                     ALARAIDERTEEHEEKISLKVRGTAREWKKRLLSRVEAAGVTLEQLERASVRHPFAEE
                     VLFGDRRFATPSGKVNLVHELPTAPPEEPAFPLWLFSNSTEQSQSSQWAMPEPDLLHA
                     TCHPSAAPGFADGEVIRLESAVGAIRVRLRFDPAQRPDVVLVPKGGSFDRGNCANALV
                     RARLTDAGEGAAYLDCRVRFAKA"
     gene            complement(16265..16942)
                     /locus_tag="PLB01_06580"
     CDS             complement(16265..16942)
                     /locus_tag="PLB01_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQQ77003.1"
                     /translation="MGEKVGVTLNGQKVDGWLAKPAAGKGPAVVVIQEWWGLVPHVKD
                     VTERFAKEGFVAFAPDLYHGKTTTSPDEAGRLLMELDVERAGREIAGAGAFLLKRPEC
                     ASKTFGVVGFCMGGALAQYAATSNPNVGATVSFYGGFKKVPLDWKHLASPLLLIYGGK
                     DTGVPASDAKPLEEKLRAMGKDVETVVYPDAGHAFFNDSRANVYDPKASVDAWKRTLA
                     LLRRALV"
     gene            16996..>18047
                     /locus_tag="PLB01_06585"
     CDS             16996..>18047
                     /locus_tag="PLB01_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQQ77004.1"
                     /translation="MAVKLFRISAIAAVAVAVGFLIACSIFFPGEPSVRKNVTGGLFT
                     DVTKASGVKFRFHGDIMDGKLIPTMGGGAALADFDGDGWLDLLLVQQVKSGSKWRKHG
                     KTQELKDCTRMFRNRGNGTFEDVTERSGVVACGWGVSAMWADLDNDGFPDLVIGNAGD
                     PNLVFRNKGDGTFEKMENTGLEGGKFTIGLMALDIDGDGLPDVYLGNYLETDSLRESK
                     APATSFMTPDEYKGQDNQMLRNLGGWKFADVTDASGTRDPGSKTIGAVALDFDGDGKT
                     DFYVANDQWRNSLFHNEGGGIFRDVSDETGTGFPEEGSVTAFGRKTRSGMGLVATDFD
                     GDGRPDLYVTNYANEP"
BASE COUNT         2705 a         6336 c         6272 g         2734 t
ORIGIN      
        1 ctccttcgag gagaccgacg cgctcgaggg aatcctcggg gagttacagc tcgtcttcac
       61 gcggctcgcg gccggcgccc gtcccaaggg tccggtcccg ccgccggcgg cgaggcgcgg
      121 ctgagaactc ccgcggaccc ggccgggctc gtccgcccgc gggtgtgcgc ggctctggct
      181 gcctgcgctt ttctcgctct cggagccgcg ccggcgctcg acgcccaggt cctgatggcg
      241 ccgggcgaaa gcacggagac ggcctttcgc aagcaggtcc gcggccagtg gttcggctgg
      301 ctcgcggcgc aggaagaggg cgacacggcg ctcgcgcgcc gcaaggtcga cgagatcctc
      361 aagcacgccc agaagatcga gatccggcgc atgacggacc tcgcgctctc ggccacgctt
      421 ctcgggcggc gccagctcgt ggccgggaaa cccacgctcg cccgtgaggc cttcgcggcc
      481 gcgatacgcc tcgatcccga cctgcccgag ccgcgctggg cacgcctcgg cctggcgttc
      541 cgggcccgcg agcccggcga aatcgtgagc ggcctcgcgg gcgcgctgcg cgcgaccgtt
      601 gccgacggcg agtcgcgccg cgtcaacctc gcgcgagccg tcctgctggt cctcgggacg
      661 gtggccgcgg cgacgctcgc cgccctcgtg atcctgctcg tccggcacgg cgggcggctc
      721 gttcacgacc tgaaggaaac ggcgggcggc ttcctgcgcg gccgcgcgac ggtcgccctc
      781 gtcctcggtg tgctcgtgct gccgctgctt ctcggcctcg acgtggtctg gctctcgctc
      841 tttctcttcg tcctcgtgtt cggctacgcg tcgctcgacc agaagatcgc gctctcgggc
      901 gccctcgtcg tcctggtcgc cctgttcccg gccctggacc gattcgccta cgagttcgcg
      961 atttcctcgt cgccgatcct tcgcggcgcc gaggccctgc aggaggcgcg ctacgaccag
     1021 cgcgtcctcg acaacctcga ggccgcgaag aacatcgccc cggacgacgt ggacatccga
     1081 ttcctgctcg gccgcctcta ccagtctctc ggccagaacg accgcgcgac tttcgagtac
     1141 accgcgggcg cgcagctcgc gccgcgcgac acccgctgtc tcgtgaaccg gggaaacatc
     1201 cggttcgtcg acggcgacct cggctcgtcg caggaggatt accagcaggc cctgaagcgc
     1261 gatccgcgct cgatcgcggc gcggtacaac cttgcgctcc tctacgccga gacgttccgc
     1321 accgtggagg cggcccagac gctccaggag gcccgggccc tcgacgcccc cgccgtccag
     1381 cgcttccagg acacgcccac gctcgtgaag gtcgtctcga tcgggtactc gaccgacgag
     1441 gcgcgccgga agatcgagga gatggagcgc gtcgggcgca gccggcgcct cctgggccat
     1501 ttccgctcgt gggacccgtt cggcggactc cgcgtcccgc tcgtcgtcgc gttcctcgtc
     1561 gcggtcgcgg cggcatgggc gctcgactcc ttccgcaagc gcggccgcgg ctacgcgtcg
     1621 gaatgccaaa agtgcggccg cgccttctgc cgcctctgca agccctcggc cgagagcgcg
     1681 ctcctctgtt cgcagtgcat ccacgtctat ctgaaaaagg acggcgtcgc gatcgagacg
     1741 aagctccaga agctcgacga cgtgcggcgg cgcaagtcct tcgaggagcg cctccgcggc
     1801 atcctgaacg ccgtcttccc gggagcggcg gcgtttctcg actccagact cccgctcgcg
     1861 tccctcgcat tcggcttgtt cgccttcggc ctcctcgcgg cgttctgggg cccgcgcctc
     1921 gccgtgctcc cgcgtcccac gctttccggc ggcgtactcg cggtcgcatt ctggtgcgcc
     1981 gtcgccctcg cgggatggct cgtcggggcg ttcacgtccc cccggaaggc ctgacgatgg
     2041 cgctcgaagg cactcttcgc gatttttcgc tggcggacat cctccagctc atcgcgctgc
     2101 agcacaagac cggcctcctc tccgtgcgga gccccggcga cacggtcacg ctcggctttg
     2161 tcgacgggca gctcgtcgcg gccgaatcct cggcgcagcg gctcgacacg cggctcggca
     2221 ccgtcctcgt gaagacgcgc cgcctctcgc ccgaggcgct ccagcgcgcg ctcgagatgc
     2281 aggcccagac tctccagcgc ctcgggttca tcctgctccg caacggcttc tgctccgctc
     2341 aggacctgag cgacggcctc gacatccaga tccggcgcat cgtctacggc ctcttccggt
     2401 ggacggacgg ggactacgtc ttcgagcaga cggaccacgt cgattacgac cccgagtcca
     2461 cgacgccgat ctcggtcgag cgcctcctca tggaaggcgc ccggatgctc gacgagtggc
     2521 ccatcgtcga gaaggtcgtc tggtcgctcg acctcgtcta ccaaaaggtg ccggtcgccc
     2581 agcccgtcat ccccgccgag gacgaggacg aggtcgaggt cgtcgaggac tcgaccgtcg
     2641 cgcgccgcgc ccgggacagg cgccccgagc cgatccgcgt ctcgcgagcg gagtggtccg
     2701 tctacgagct cgtcgacggc cagcggacgg tcgcggagat caacgagctc gccttcctct
     2761 cggagttcga cggttgcaag gcgttcttcg acctcgtgag ccgcggcctc gtcgaggagg
     2821 cgatgccgcg agccgtcgag gcgcccgacg aggcgggggc gaccgtcgga gtcccggccg
     2881 cacgggtgcc gcgcgccccg ctcgatctga acgtcatcgc gggggtgggt ctcgcgctcg
     2941 tcctcgcgtt cgccgtgagg atgcaatcga agaaccctct caacctcgtg acggcgccgc
     3001 cgcggcgcga cgccgtcgtc ggcggctacg agaaatccct ctcgctcctc aagctgcggc
     3061 ggctcgccga aggcgtcgac acgtacacgc tgatctccgg gcggcttccg gacgggctgg
     3121 attccctcgt cgcctcccag atcctccgca tgcgagacct cgaggacccc tggggcacca
     3181 agtaccgcta catcgtgcaa ggtgagaagt tctatctcgt cgggttcgac gccgacgggc
     3241 acaccgaccc ggacctcctc ttttccagga cgctcgtcct gagggagacg aaggaacagg
     3301 agctcgcgcc tcactcgaca cgggaaatta ctgtagttga atgagttgac tgatctaacg
     3361 cgcggctgta ggtgtgtgtc tttcgagact tgacaacaac acgctaagat cctatcttac
     3421 ccgtcggctc gccgggcgag ccagaggagt ccaggtcaaa tgagcaagat catcggcatc
     3481 gatctcggca cgacgaacag cgtcgtggcc gttctcgaag gaggcaccgc ggaagtcgtc
     3541 gcgaacagcg agggcgggcg gacgacgccg tccgtcgtcg gatacgcgaa gacgggcgag
     3601 cgtctcgtcg gccaggtcgc caagcgccag tccgtgacga acccggagaa cacggtctac
     3661 tcgatcaagc ggttcatggg gcgccgcttc gacgaggtca acgacgagat gaagatggtg
     3721 ccctacaagg tcgtccgttc ctcgaacggc gacgcgcggg ttttggccgg gggcaaggag
     3781 ctttctcccc ccgagatctc cgcgatgatc ctcacgaagc tcaaggaggc cgccgaggcc
     3841 tacctgggcg agaaggtgga tcgcgcggtc atcacggtgc ccgcgtactt caacgacgcg
     3901 cagcgccagg ccacgaagga cgccggccag atcgcgggcc tcaaggtgga gcgcctcgtg
     3961 aacgagccga cggcggcggc gctcgcgtac ggcctcgaca agaagaagaa cgagctcatc
     4021 gccgtctacg actttggagg cggaacgttc gacatctcga tcctcgaggt cggcgacggc
     4081 gtcgtcgagg tcaagtccac gaacggggac acgcacctcg gcggcgacaa catcgaccag
     4141 cgcatcgtcg agtggctcga ggccgagttc aagaaggacc aggggatcga cctctcgaag
     4201 gacccgatgg cggtccagcg cctcaaggag gccgcggaga aggcgaagat cgagctctcg
     4261 acgaccgtcg agaccgaggt gaatctgccg ttcatcacgg cggacgcctc cggcccgaag
     4321 cacctcacgc tcaagctcac gcgcgcgaag ctcgagtctc tcgtggacga catcctccag
     4381 aggtccgtgg gaccctgcaa gcaggcgctc aaggacgcgg gggtcgacgc ctcgaagatc
     4441 gacgagatcg tcctcgtggg cggccagacc cgcatgccga agatccagca gatcgtgaag
     4501 gacttcttcg gcaaggagcc caacaagagc gtgaaccccg acgaggtcgt cgcgatcggc
     4561 gcggccatcc agggcggcgt tctcgcgggc gacgtcaagg acctgctcct cctcgacgtg
     4621 acgcctctct cgctcggcgt cgagacgctg ggtggcgtca tgacgaagct catcgagcgc
     4681 aacacgacga ttcccgccaa gaagagcgag gtcttctcga cggcctccga cgggcagacg
     4741 tccgtcgaaa tcaaggtcct gcagggcgag cgcgagatgg cgggcgacaa caagctgctc
     4801 ggcgtcttct cgctgatcgg catcccgccg gcgccgcgcg gtcttcctca gatcgaggtc
     4861 acgttcgaca tcgacgcgaa cggcatcctg aacgtctcgg cgaaggaccg cggcaccggc
     4921 aaggaacaga agatcacgat cacgtcctct tcgggcctcg acaagaacga ggtcgaaaag
     4981 ctcgtgaagg acgcccaggc gcactcgggc gaggacaaga agcgccgcga ggccatcgaa
     5041 gccaagaacg ggctcgacgc gctcgtctac cagacggaga agatcctcga ggagagcggc
     5101 gacaaggtgc cggcggccga gaaggacgcc gtgacggccg cggtcgccga tgcgaagaag
     5161 gtcctcgaga gccagtccgg cgacgccgag gccctcaaga aggcggccca ggagctccag
     5221 aaggcgagct acaagatcgc ggaggccctc tacaagcaga ctcctccgcc ggccggcggc
     5281 gcctccgagg gtggagcgcc cggcggcccg gccggcgccg ggaaagagga cgtcatcgac
     5341 gccgaggtcg tggaggagaa gaagtaacgc cccatggacc gcgaggaggg ctacgtcttc
     5401 atcggcgtcg tcgcgaaacg cttcggcgtg catccgcaga cgctgcgcct ctacgagcgc
     5461 gagggcctca tccggcccgc gcgctcgtcg gggaacacgc gcctgtacga cgccgacacc
     5521 gtcgagcgcc tcgagatcat cctcacgctc acgcgcgagc tgggcgtgaa cctcgcgggc
     5581 gtcgaggtca tcctcgatct caagtcccgc ctcgcccgga tggagggcga ggtcgggcgg
     5641 ctcatggagg ccatccgggc ggtcgcaaag gaccacgccg cgcgcgccgg ggccggcccg
     5701 aaggacgaca aggccctcgc cgtgcggccc cggggggcca tcgtcctcaa gtacgactga
     5761 gggataccgc gcctcctccg gagaggtgat aatcaccggc cggtgacttc aaggctgcgc
     5821 cccgacgacg actccaaggt cctccggaag tacctggacg acatctcccg ctacccgatc
     5881 ctcgcggcgg agcgcgagcg ggagctcggg cggatcatcc aggacctttc cacgcccgaa
     5941 gaggcgcgca aggaggccgt ggacgagctc gtgcgcggaa acctgcggtt cgtcgtctcg
     6001 tacgcgaagc gcttccactc gcccgaggtc tcgtttctcg acctgatcaa cgaggggaac
     6061 atcgggctca tccaggccgc gaggcggttc gaccccgagc gcggcgtcaa gttcatcacg
     6121 tacgccgtgt ggtgggtgcg ccaggcgatt tcgaacgccc tctccgagca gtggggcgcg
     6181 atccgcctcc cgcacaagca ggcgacgctc cactcgcgcc tcgggagggt caaggaagcg
     6241 ctcacgcgcg cgctcggccg cgagccgacg atggacgaaa tcgcggcgga ggcgggcttg
     6301 aaggtgatcg acgccgagaa cctcatgggg atgacccgca gctcggagtc gctctccggc
     6361 gtcttcggcc tcgaggagga ccgcacgctc ggggacacgc tcgagcagac gagcgtcgcc
     6421 gccgcggacg agcagctcct ccggcgctcg tcgatcgacc agacgcgcac gctcctcgat
     6481 gcgctcccga agaaggagcg ggcgatcctg tgccgccgct tcgggatccc cgaggacggc
     6541 acggagggcg aacgcgagcc catgacgctg caggagatcg gagaggaatt gcatctctcg
     6601 cgggagcgcg tgcgtcagat cgaggcgcag gccatcgccc gcatcaagcg gtccttgaag
     6661 agccgcgcgc tcaaggcgtt cctgaactga tgtccaccgt cgccgctccc gaaccctgcg
     6721 tcctctgcaa gggcttcggc gtggtcgaga cgcccgaggg ccgggtcgtc gcctgccggt
     6781 gccgcctcgg agacctcgcc gaggcgcgcc tgagggccgc gcagatcccc gagcgatacc
     6841 ggaactgcac cctcgagaac tacatcgtcc acaaggacgc gcccccgtcg cagcgcgtgg
     6901 cgaagatgaa gtcggtgaag ttcgtcgagg agtggaagtc cgtccgctac gggcttctct
     6961 tcatcggcac ggtcggcgtc ggcaagacgc acctcgccac ggcgatcctg aggcgcctga
     7021 tcgaggagtg cggcgcgaag ggcgttttct gcgacttctc ggacctcctc gagcgcatca
     7081 aggcgacgtt ctcgaagacg aaccccgaca gcgccgacga cgtcctcgcc ccgtaccgcg
     7141 aggccgagat cctcgtgctc gacgagctcg gcgcccagcg cccgaccgac tgggtgcgcg
     7201 acgtcctgta cggcctcatc aacacgcgct acaaccgcca gcgcatcacg atcgtgacct
     7261 cgaactacgg cgacgcgccc cggaccgcgg gggaggagac gctcgagagc cgcgtcgggg
     7321 tcctcgtccg cagccggctg cacgagatgt gcgtccccgt cctcatcgaa ggcgacgact
     7381 accgcaagaa gcagcgggcc tggagtgcct gaaacgggtg cccccgctcc ccgcacgcgg
     7441 tccgcgcccg cggcgttcgg acgcggggtc tcgcaggtcg tcgccaatcc cggcctcctc
     7501 ctcgcgccgc tcgcgttcgg cgggatcgtc gccgcgtcga tcgtggccgc ggccttcgtc
     7561 gcggcgttct ggttcggccg cgcgttcgcg tcggctgcgc ggatggccga acgcgggccg
     7621 ggggctcttt cggatctcct ggaaggtctg cggggcctgc ttttcgctgc accgggcgcc
     7681 gtcgtgctcg cccttttggc cgttctcgtc ggactccttc tcctgacggc tctcgccgcg
     7741 tggatccgcg ccggcgtcac ggggagcctc gcggagatcg acaggcgcgc gggtgagggc
     7801 gcgccgctgt cggcgttccg ccacccgatg ctcggcccgc tgttcttcgg ctcggcgggg
     7861 cgcctcttcg ggggcttctt cgcgctcgtg aacctgtacg cgctcgtcgc gtcgttcgcc
     7921 gtccttctga tcgtcctgcc tctcgtcggc gcgttcgtgg cggtcgaggc cggccgctcc
     7981 ggcctcgtcg tccttttcgc cctcctcttc gtggtcctcg ccctcgtcgg gatcgtcgcc
     8041 tcggcggctc tgcgcgtcgt ttatctcgtc gccggccgcg tgctcgcgat cgagggtctc
     8101 gacgccctgg cggcggcggg gcgcgccatc gcgctcgtga gggcgtctcc gggccgcgcc
     8161 gcgacgctct acctcctgac ggtcggcggc gcgatggccg tcggcctcgc gttcgtcttc
     8221 ccgcggatcg tcctcacgtt cgcggccggc tggacggacg cgggcgcctg ggccccggtc
     8281 ctcgtgagcg gagcgttcat cctcctgcag atgggcgccg ggttcgctta cgacctttcg
     8341 gtgacgggcg cgttcgtcgc gctgtggccg gcggacgagg cgaaggagcc gcccctgccg
     8401 tgaagaggct cgccctctcc gccgtgctgg cgatcgtcgc cgccctgggg ggctgcgctt
     8461 cgccgtccgc tcccgtcgtc gcgccccttg ccgccacgcc ggcggtcccg ccgactcccg
     8521 tcccgagtcc cgttccgacg cccgcgtcgg ctccgcgcct cctgccgctg ccctcgcccg
     8581 cgctgaacgt gagcctcgcg gtggaccgct cctcggcggc gtttccggcg ggagactggg
     8641 tgcttgccgc gggggagcgc gtcgagcgcc gacggggtcc cctcgtcgtc gcgcccgcga
     8701 acggaggact gcgcgtcacg gacgacggcg ttagctccga ctggccctcg cccgtcgaga
     8761 tcgcccctgc aaacggggcg ctcgtgccgt gggccgacgc gacgtaccgc ggccggctcg
     8821 ccctgcgcgc aaccgttcgg gggacgctgc acgtcatcaa ccgggtcggt ctcgaggact
     8881 acctgaaagg cgtcgtcccg gccgagatgg gaccgcgcgt gtacgacgag gtcgaggccc
     8941 tcaaggcgca ggccgtcgcg gcgcgctcgt acgcggcgaa gcaccgcggg cagttcgcgg
     9001 ccgagggcta cgacctctgc gcgaccccgc gctgccaggt ttactcgggg gcttcggccg
     9061 agaacccgct ctcgacgcgc gccgtcgagg agacggccgg ggaggtcctc ctctggaagg
     9121 gcgcggtcgc cgacgccctc ttcacgtcca cgtgcggcgg gcgcacggag aacgcgcccg
     9181 acgtcatggc cggctacgcg aaggtggacg ccccgtatct cgcgtcggtc gcgtgcttcg
     9241 gcgagacgcc ggtcctgctc gagtcctccg ccgtggcgaa gaaggggacc gtcacgctgc
     9301 tcggagcgcg cggccgcgcg ctcgccgcga gtttcgaaga gggagaggga gcgggagaag
     9361 gagcgaagat tcctcagaag gcgcgtgaag cgctccagcg gaggctcggg gtcgccgcgc
     9421 atggggcgtc gcgtccccat cccctctcgg tgcccgccgt ctatctggag atcgcatccg
     9481 ctgcgggctt cgacaccgcg gtgctgagcg aggagagcga tcgaggagaa ttgccttcga
     9541 attggtcgga aaaggcgaag gcgtcgttcg ctgcgctgca gcggttccag ctgggcggcg
     9601 ggagtgcgct cccgaccgac cgcgccctga agcccgagga agccgccggc atctatgccg
     9661 tcctgctctc caggatgggc gacttcgagg acgtcgaagg ccgaatcacg aagagcgacg
     9721 acaccggaat cgaggtgaag ggcgccaagg gacggtcttc gtattcactt tcaaagaaat
     9781 cttcggtgct cttcgaaggc ggcaacgagg cgctgatcca ggttgcatcg caccggttct
     9841 tccccggcga ccgcgtccgt gttttcgtcc gggacggcaa ggcgctcggc gtcgtcttcc
     9901 cgcccgcgcc cgcggccggc gcgtacgacc gcgagtcggc ctgggagcac tggacgcgcc
     9961 ggttcaccgc gaaggacctg ggcgcgaaga ttcgcgagcg cgaggggacg cgcacggcgg
    10021 cggacccggc ggggatcgcc gtgacgtcgc gcggcgtctc cgggcgcgcg cgcaccgtcg
    10081 ccgtcgcgac ggcgaagggc acggtcacgc tcacgggact cgagatccgt ttcgcgctcg
    10141 gcctccccga gagcctcttc aacgtcgtcg cgggccggga cgaggacggc gagcgcgtct
    10201 tcaccttcta cggccgcggc tggggccacg gcgtcggcct ctgccagacg ggcgcgttcg
    10261 gcatggcgct ttcggggcgg acgtaccgcg agatcctcgc gcattactat ccgggggccg
    10321 aggtcgcgca ggttccgtga gaaagagcgc cctacgggcg cccgaggatt tcttcgaatg
    10381 agaagagatg acggtttgcg cctctcgccg gagcagcggc gggaccttct cgtgcgggcg
    10441 cgcggcgccg cggcgagggc cctcggaatt cctttcgctg gaattcttcc ctctcttccc
    10501 tcgggccgtc tcgccgagcc ggggatgtgc ttcgtgacgt ggaagcggga cgggcagctg
    10561 cgcgggtgca taggaagcgt cgagcccgtg cgtccactct gggcggacgt ggaagccaac
    10621 gcggtccacg ggctcctcga agacccccgc tttccgcccg cgacgccgga cgagctcgcg
    10681 cacctccggc tcgagatctc ggttctcacg ctgttcgagc ccgtggccga tccgcttcgc
    10741 gacgtgagga tcggcgtcca cggcctgctc gtcgagcgcg gcccccaccg cggtctcctc
    10801 ctgccgcagg tgcccgtgga gtgggggtgg gacgttcccg cgttcctcgc gcactcgtgc
    10861 cggaaggcgg gccttcccgc cgacgcatgg cgcgatccgc ggacgacggt ctcgacgttc
    10921 gaggccgagg tcttcggcga acaggacggc gccgcgggcg gataatcgcg gccatgccgt
    10981 ccgccgcgac ctccgccgac gtcgcctccg tcgtctcgat ccgcccgcac ttcccggccc
    11041 tcgagcgccg cgaaggcggg gcccccgtcg ccttcttcga cggcccgggc ggcacgcagg
    11101 taccgcgcgc cgtcgcggac gcgatgacgg actatctcct ccgccacaac gcgaacacgc
    11161 actgggtcta cccgacgagc gtcgagacgg acgcgatgat cacggcgtcg cgcgccgccc
    11221 tcgcggactt cctcggcggc gacccgtccg agatcgtctt cggcgcgaac atgacgacgc
    11281 tgacgtttca cctgtcgcgc gccctcgcgc gcggctggca gcccggcgac gaggtcgtcg
    11341 tgacggagct ggaccaccat gcgaacgtcg acccgtggcg cgctctcgag agggacttcg
    11401 gcgtcgtcgt gaaggcggcg ccgctcgtgc cgggggaggc gaggctcgac ctcggcgccc
    11461 tcggccggct cctgacgaag aaaacccgcc tcgtcgcggt cggcgcggcg tcgaacgcgg
    11521 tcgggacgat caacgacgtc gcgcgcgtgg ccgccctcgc gcgcgcgaac ggctccctcg
    11581 cgttcgtgga cgccgtccac tacgcgccgc acgcgctcgt ggacgtcaag gcgctcggcg
    11641 cggacttcct cgcctgctcg ccgtacaagt tctacgggcc gcacgcgggc gtcctgtggg
    11701 ggaagcgcgc tctcctcgag gcgctcgacg ccccgaaact gcgccccgcc cccgagaacg
    11761 cgccggaccg tctcgaaacg ggcacccaga accacgaggg catcgccggc tgcggcgccg
    11821 ccgtcgactt cctggcctcg ctcgccggag gcggcggaga ccgccggtcg cgactcgcgc
    11881 ggacgtttgc cgctcttcac gcgcgcgggg aagagctcct gtcgcggatg tggaacggtc
    11941 tctcgcaggt tcccggcgtc acgctctacg gcccgccgcc cggcgccccg cgcacgccca
    12001 ccgtcggctt caccgtggag ggctgggatt cggcggcggt tgcccgtgct ctcgtcccgc
    12061 acggcgtcta cgtttcgaac ggcgacttct atgcgacgac gctcgtcgag aagctcggcc
    12121 acggggccga cggcctcgtc cgcgcggggg ccgcctgcta cacgacgccg gacgaggtcg
    12181 accgcctcgt gtcggccgtc gcccgcctat cgccgcgcgg gcattgatgc taaggttcct
    12241 ggaaatttga cgcctctgtc aaacacagtc gcgtcggcag gagtccccat ggccgccccc
    12301 gtcgcttccg ccccgcccgt caccggattc ggattcgccc tcaccgaaga gcaggaagcc
    12361 gttcgcgcga tggtccgcga cttcgcgcgc accgagatcg cgccccacgt gatggagtgg
    12421 gacgaggccc agacgtttcc tcgtggcgta atggcgaagc tcggcgagct cgggatgctc
    12481 ggcgtcctcg tgcccgagga atacggaggc gcgggcctct cgtacctcga ctacatccac
    12541 gtcgtcgacg aaatctcgcg cgtggacgga tcgatcggcc tcggggtcgc ggcgcacaac
    12601 agtctctgca cgaaccacct ctggcttttc ggcagcgagg cgcagaggcg gaggtgggtc
    12661 acgccgctcg cgcaggggaa actcatcggc gcctggggcc tcacggaggc cgaggccggc
    12721 tccgacagcg gcggcgcgaa gaccacggcc gtgaaggacg gcgactcgtg ggtcctgaac
    12781 gggtcgaaga acttcatcac gcacgcgtcc gtcggcgacg ttgcggtgct gctggccgtc
    12841 acggacaagg ccgccggccg gaaccggatc tccgcgttcg tcgtggacct gcaccagaag
    12901 ggcatccacg ccgggaagaa ggagaacaag ctcgggatgc gcgcctccga cacggcgact
    12961 ctcgtcatgg aggactgccg catcccggcg gagaacctga tcggcgctcc gggcgaaggg
    13021 ttcgtccagg ccatgaaagt cctcgacggc ggccgcatct cgatcgcggc gctcgcgctc
    13081 gggattgccg agggggcttt cgagggcgcg cgcgcctacg cgcagcagcg gaagcagttc
    13141 ggcaaggcga tctccgagtt ccagacgatc cagaactacc tcgccgacat ggcgaccgag
    13201 atcgacgccg cgcgcctcct gatctaccgg gccgggtgga tgaaggacgc cgggaggatg
    13261 acgacgaagg agtccgcgat ggcgaagctc tacgcctccg aggtctccgt ccgcgtctcg
    13321 aacctcgcgg tccagatcca cggggggtac ggcttcacca aggacttcgg cgccgagaag
    13381 tacttccgcg acgccaagct ctgcacgatc ggcgagggca cgtccgagat ccagcgcctc
    13441 gtgatcgcgc gcgagatcct gaagaaggcg gccggcgcct agcgccggtc gaggacggcg
    13501 cgcccctctt tcatgacgaa gagaggcgac tcgatcgttc gcacgttctt cagcggattc
    13561 cggaggctct cgaccttcag actctgtacc tcgcgcgact tccggagctg gtagtcctgg
    13621 tagccgccgg cggcgccgcg ctccagcatg aatgcattcg tctgcgggtt cgagacgacg
    13681 aacgtcccgt tcgccttgag cgccgcgatc gtaggggcgg aacccagccg cgcggactgc
    13741 gtctctgttt cggaggtgac gcatgagggt ttcagctctc gcgatcgcgg ttctcctcgt
    13801 gtccgggacg aaggggtcat cggccgggcc ggagccgccg cggccgtcgt tcgcggcgcg
    13861 ggccgtcccg aaaggcgcgc tcgcccaggc cgtcacgccg gccagctccc tcgccgccga
    13921 gcgcctcggg accagcgcct gccgcggaat cttcgaggaa ctgccggact tcacgggccg
    13981 gcccgtggcc cgtcgcctcg aggccggcga gcggagccct tcttcgcact tcgcgcggct
    14041 gacgttcctg gggagcaccg agggtccctg ccggtacagc ggcacggcgg catggtccgt
    14101 ggccggggac actcgtgtgc gcgtctgtgc gcggacgttc ggcgcggtcg cggcccacga
    14161 ccggcgggaa gccgctgcgg tcctcatcca cgaggctctg cacacgctcg gcgtgggaga
    14221 ggacgcggcc aaggagcctc tgacggcctt cgtccgccgc cgttgcggcc tgtaggcgtc
    14281 aggccttcgc gaacctcacg cggcagtcca agtacgcggc accctctccg gcgtccgtga
    14341 ggcgcgcccg gacgagggcg ttcgcgcagt tcccgcggtc gaagctgccg ccctttggga
    14401 cgagcacgac gtccgggcgc tgcgccggat cgaaccgcag ccgcacgcgg atcgcgccga
    14461 ccgcgctttc gaggcggatc acctctccgt cggcaaagcc gggcgcggcg gaagggtggc
    14521 acgtcgcgtg gaggaggtcc ggctcgggca tcgcccactg cgagctctgc gactgctccg
    14581 tcgagttcga gaacagccag agggggaacg cgggctcctc cggcggagcg gtcggcagtt
    14641 catggacgag gttcaccttc ccggaaggcg tggcaaatct gcggtctccg aagaggacct
    14701 cttctgcgaa gggatgtcga accgatgcgc gctcgagctg ctccagcgtg acgcccgcgg
    14761 cctccactcg cgagagcagc cgtttcttcc attcgcgggc ggtccctctt actttcaaag
    14821 aaatcttctc ttcgtgctct tcggtcctct cgtcgatggc gcgggcgagc gcctggacga
    14881 tctcgaggtc ggtcaggacg cccgccggcg gcgcgacgac cggcgtcgag gcgccgatcc
    14941 agtggtggcc gtacgagccg agaaggtcgt cgtcctcgac gagcgtcgtc gtggggaaga
    15001 cgaccgtcgc ccgccgggcc gtgtccgtga ggaacggatc gacgacgacg acgagctccc
    15061 gcgtctcgag cgcccgcgcg acggtggccg aatcggggag catggcgacg gggtttcccg
    15121 cggtgaccca cacggcgcgg atcggcgggt cctgcgcggc caggatggcc tctccgagga
    15181 gcggctccgg aaacgtccgc gggtgggact tcgagagaaa cgagaagtcg aacggggccc
    15241 ggcgcacggc ggagaagaaa acgccgccgc ccgggatgcc gagattcccg gtcacggcgc
    15301 cgagggcgtc gagcgcgcgg acgatcccgg cgccgttcat ccgccggccc atgccccagc
    15361 cgacctggat cgagcacggc cgggccgcga gcgcggcggc gagcgcatcg acgtcgtccg
    15421 gcgcgacgcc ggcggctccg gcccacgccg ccgcggtccg ggagagggcg agcgcgcgaa
    15481 atgcgtcgaa gtggtcgcag tacgagggcg cggccgagtc gatcccgccc gtctcgaaca
    15541 gccgtgcggc cacgcccatc gcgaggtcga aatcgccgcc gggacgcggg aggatcgtcc
    15601 ggtccgcgag cttttcgctc ttgtgacgta tcgggtcgac gaggaggatg cgcgcgccct
    15661 tgcgcttcgc ctcgcggagg atcggctgca ggtggacgtt cgagaccgcg ggattcttgc
    15721 cccagaggat cacgttccgg gcattcgcga cgtcgaagat gtcgtgcgct tcctcgcgcc
    15781 cgaagtcgat gacctgcgcc gcgtcgcccg cgccggagca gatgtcgccg cgcttgccgc
    15841 tcacggggcc gagggcctcg aagaagaggt cgacgaccga cttcacgacg ccgagcgagc
    15901 cgcccgagcg gtagtggagg atcgcagcgg gtcccgactc cttccggatg gcgaggagcc
    15961 ggtccgcgac gaagccgagc gcctcctccc acgtggcggg ggcgagcacg ccggtcttcc
    16021 tgatcagggg cgtcgtgagg cgaagcggcc cgttctgcat ctcggggaag cgcgaggtcc
    16081 ggtagcagag gaagcccttc gtgaccgggt gggacgggtc tccgccgagg gcgacgagtt
    16141 ttccgtcctc gacggtcgcg atcaggccgc aggcgtcggg gcagtcgcgg ttgcagacgg
    16201 tgcgatgctc gagggcggcc atggccccat tctagagaga cggcgtctcg cccgggaggg
    16261 cggcctagac caaggcccgg cgaaggagcg ccagagtcct cttccaggcg tccacggacg
    16321 ccttcgggtc gtagacgttc gcgcgcgagt cgttaaagaa cgcgtggccg gcgtcgggat
    16381 agacgacggt ctcgacgtcc ttgcccatcg ccctgagctt ctcctcgagg ggcttcgcgt
    16441 cggaggcggg gacgcccgtg tccttgccgc catagatgag aagcagcggg gacgcgaggt
    16501 gcttccagtc cagcgggacc ttcttgaagc ccccgtagaa gctcaccgtg gcgcccacgt
    16561 tggggttcga cgtcgccgca tactgcgcga gcgcgccccc catgcagaag ccgacgacgc
    16621 cgaacgtctt cgacgcacac tcgggccgct tgaggaggaa cgcgcccgcg ccggcgatct
    16681 cccggcccgc gcgttcgacg tccagctcca tgaggaggcg accggcctcg tcgggcgagg
    16741 tcgtcgtctt gccgtggtag aggtcgggtg cgaaggcaac gaagccctcc ttcgcgaagc
    16801 gctccgtcac gtccttgacg tgcggcacga gcccccacca ctcctggatc acgacgaccg
    16861 ccggcccctt gccggccgcc ggcttcgcga gccagccgtc gaccttctga ccgttgagcg
    16921 tgacgccgac tttctcgccc atgacgtgcc ctccgtccgt acctccggtg aatcctatac
    16981 tccgatcagg tgagcgtggc cgtgaaactg ttccgcattt ccgcgatcgc ggccgtcgcc
    17041 gtcgccgtcg gcttcctgat cgcctgctcg atcttcttcc cgggcgagcc gtcggtgagg
    17101 aagaacgtca cgggaggcct cttcacggac gtgacgaagg cgtccggcgt gaagttccgg
    17161 ttccacggcg acatcatgga cggcaagctc atcccgacga tgggcggggg cgcggcgctc
    17221 gcggacttcg acggcgacgg ctggctggac ctcctcctcg tccagcaggt gaagagcggc
    17281 tcgaagtggc gcaagcacgg aaagacgcag gagctcaagg actgcacgcg catgttccgg
    17341 aaccgcggga acggcacgtt cgaggacgtc accgagagat ccggggtcgt cgcctgcggc
    17401 tggggcgtga gcgcgatgtg ggccgacctc gacaacgacg gcttccccga tctcgtcatc
    17461 gggaacgcgg gcgacccgaa cctcgttttc cggaacaagg gtgacggcac gttcgagaag
    17521 atggagaaca ccgggctcga gggcgggaag ttcacgatcg gcctcatggc cctcgacatc
    17581 gacggggacg ggctgcccga cgtctacctc ggcaactacc tcgagacgga ctccctccgc
    17641 gagtcgaagg cgcccgcgac gtccttcatg acgcccgacg agtacaaggg ccaggacaac
    17701 cagatgctcc ggaacctcgg cggctggaag ttcgcggacg tgacggacgc gagcggcacc
    17761 cgcgacccgg ggagcaagac gatcggcgcc gtcgcgctcg acttcgacgg cgacgggaag
    17821 accgacttct acgtggcgaa cgaccagtgg cggaactcgc tctttcacaa cgagggcggc
    17881 ggcatcttcc gcgacgtttc ggacgagacg ggcacgggct tcccggaaga ggggagcgtc
    17941 acggccttcg ggcggaagac gcgcagcggc atgggtctcg tcgcgaccga cttcgacggc
    18001 gacggccgcc ccgacctcta cgtcacgaac tacgcgaacg agccgaa
//
LOCUS       DAOMFY010000054        17820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_992_length_17820_cov_8.606924, whole genome shotgun sequence.
ACCESSION   DAOMFY010000054 DAOMFY010000000
VERSION     DAOMFY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17820
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_992_length_17820_cov_8.606924"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLB01_06590"
     CDS             complement(<1..185)
                     /locus_tag="PLB01_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77005.1"
                     /translation="MLFLSKKIFSSLLLSSAVLAASAARAQAPAAATPAPPKYPDYPS
                     EIPATFKPTFDGFDYERR"
     gene            256..2076
                     /locus_tag="PLB01_06595"
     CDS             256..2076
                     /locus_tag="PLB01_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQQ77006.1"
                     /translation="MRPPPFRSIPDLFFARVEATPDAPAFLHPEDGAWKSLSWRDTAS
                     RVRELACGLLSLGISAGDRVAIASSTRIEWILADLAVACAGGVTATVYPVSTEDDTSH
                     ILSDSRAAIAFAEDDAQARRILSRRGGLPFLRNLVLFDASAGLPGDAMSLRDLAALGR
                     EWDAAHPGGYEAAAGAAGPSDLATLIYTSGTTGRPKGVELPHDCWLFEIESIGSLGLL
                     GPSDLQFLWLPLSHVFGKVCVTLGIAFGFPTAVDGRVDRIAANLSSVRPTFVCVVPRI
                     FERVHAKILEQARAGGKTKAAIFDWALSVGVDAARRRSEGRRVGAFLGAKHRLADALV
                     FRKVRQRFGGRLRFFICGSVALARELTEFFAAFGVTILEGYGLSESTAMTVSNLPDRN
                     RWGTVGIPIPGIEVSFAGDGEILLRGRGIMRGYFGLPEETARALDVHGWLHTGDVGTL
                     DAAGHLVITDRKKDLIKTSGGKFVAPQHIEGMLKLETPLIAQVLLHGEGRRFCTALIA
                     LSEEELLAWARAEGIDAAYKDLVQDPRVVELVRRHVEHVNARLARHEAIRRWAILPGE
                     LKVETGDLTPSLKVRRRVVEQKFRDVLEGLYVEPPPPEAP"
     gene            2127..2786
                     /locus_tag="PLB01_06600"
     CDS             2127..2786
                     /locus_tag="PLB01_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77007.1"
                     /translation="MRPRATAAPLLLSWTLLVFPSPLTAAPAAAPKPSRKPLKGCKWE
                     KLTDAAAGLEVWAQRCDFGFRKIDFLFEKNALLIRYSDGGKPDPVIELLDLKDGEAVE
                     AGIQRLFSERTSRFLVSRCRLVPSAGKPPAGSKWFTFEPEPAYAAELRKKQDPNEVPP
                     PPCGDWGSATDGVQYWEAQPASGARKVLFVRVGQETPLYDERTLRLLPAAPARTAPPA
                     R"
     gene            complement(2737..3795)
                     /locus_tag="PLB01_06605"
     CDS             complement(2737..3795)
                     /locus_tag="PLB01_06605"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQQ77008.1"
                     /translation="MSSPVSSPAPAAAPSTFVFAQGREPHAVRAKEILKAHADIRKLA
                     GRNPWSAVLIAALVVFQTAAAGLLSGQPVWVILAAAWLVGAFASHALWVLIHDCAHNL
                     VVKSGPWNKVFAMAANLPHLFPSAVSFQKYHLKHHAFQGVYELDADLPNRWEARLVGH
                     SALRKALWLLFFPFFQLSRPPRLKEISLFDGWVVVNIVVQLAYDAAVLYVLGPKALLF
                     LAAAFFFSVGLHPLGARWIQEHYLVHGTNQETSSYYGFLNVPALNVGYHNEHHDFPWV
                     PWHRLPSVRRAAPEAYDTLRWHRSWTKLLFQFLFDPSISLWSRVVRADRGGVPVVFAE
                     ATVSVPAVPSVPALPAEA"
     gene            3936..6320
                     /locus_tag="PLB01_06610"
     CDS             3936..6320
                     /locus_tag="PLB01_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77009.1"
                     /translation="MQVAPGSRLGPYEVVAPVGAGGMGTVWRARDMRLDRDVAIKVVR
                     PDLLSDEMARRRFRKEALALAKLQHAHIAYILDVVTEGSVDALVMELVPGVSLSERLA
                     KGPLGEAEAVRLGIQLADALAAAHAQGVVHCDLKPANLRVTPDGDLKVLDFGLAKLRR
                     PALGGDLTAAATESATALEAVGTLPYMAPEQLRAEPVDARTDVWGAGAVLCELATGRR
                     PFEENVPALLTDAILHAEPPTLSGSGPAPSPGLREILQKCLRKEPSERYETAARLRAD
                     LDSLSKKPAEAAAASARSSASTPAWPRRRIAVTGGLLVLAAVAFGALWRSGVFPRSAA
                     GGRIDSLAVLPLSNLSRNPEQEYFSDGMTEALIAELSKIRALKVISRTSVMQYKNATK
                     PLPEIAKALGVKGVVEGSVMREGESVRITVQLIEADTDRHLWAESYTRDLKNVLSLQS
                     EVASAIARAVRVAVTPEETRRLSARRTVDPEAHRLVLLGQQAVRASASRDSFEKAAGY
                     YRRAIEKDPDDASAHLSLGAALFSIGGFGWQPVKTACGAARAEADKAAALDPDSGEVL
                     GFRAYLRYFCDRDTPGAVEGLRRALDLAPGSAVLHDQLAWILSSAGRHDEAIAESAIA
                     VELDPLHDQITVHRGMYFGQARRHAESERQFRNVLATTPDSSFAKFALGQELIAQGRY
                     DEALTTLLSRKVPRPEMNFLVGLAQGLAGRKDEARKVLDFLLERRRTQFVPPAMIAMV
                     YLGLGDKDKAFEWLDAACDEHGIFTDQAKVHPMFDPLRADPRFDALLRKMSAPA"
     gene            complement(6333..6899)
                     /locus_tag="PLB01_06615"
     CDS             complement(6333..6899)
                     /locus_tag="PLB01_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HQQ77010.1"
                     /translation="MARVRKTEKSRSRKPDTKVESARVILSLGDDVSTDVIYPGRFMA
                     TVLPSETPALAFADHAAFQALVKAKAIPKGAVIAGGENFGCGSSREQAASCLKGHEVV
                     VVARSFARIFLQNAINLGLNVVVAPGFTADEGDEVVFERGGVENRTKRTAYDVVPLPF
                     ARQAIIDAGGLIAFTRARLVAARGAAAR"
     gene            complement(6901..8190)
                     /locus_tag="PLB01_06620"
     CDS             complement(6901..8190)
                     /locus_tag="PLB01_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQQ77011.1"
                     /translation="MGATAVEKILARASGKPAVRAGDVVEPRVDLAMSHENAALVINQ
                     FREIYEGTGLTAKPWDPSRIAIIFDHRVPAESAKTATNQKKVRAFVAEHGIEKFHDVR
                     GDVGGICHQILPERGYVRPGMVVVGTDSHTTSHGALGAFAFGIGATEMASAWALGVAL
                     NIEVPPTIRVEVSGRFGKHVGPKDLILHLIGHLTAQGANFRVLEFTGETIANLSTSGR
                     LVLCNMSVEAGATAGIVPGDAETVRYLREDVGVTDPIDLVTSDPDATYEKTVSVDVSK
                     LEPLVACPHTVDNVKPVSAVAGKPVQQVVIGSCTNGRLDDLAEAAAIVKGRHVAAGTR
                     MLVFPASGKIWAEAMKLGYLSDFMAAGAVVMNAGCGPCLGVHEGALGDGETAISTTNR
                     NFKGRMGNPGAEVYLSSPAVAAASALTGVITDPRKER"
     gene            complement(8193..9323)
                     /locus_tag="PLB01_06625"
     CDS             complement(8193..9323)
                     /locus_tag="PLB01_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HQQ77012.1"
                     /translation="MSRYRIAWLPGDGVGVEVMDATRKVLDAVGLDAEYPHGDIGWEF
                     WCAEGDAFPARTIELLKNVDAAMFGAITSKPSKAALDELAPSLRTQGLVYRSPIVRMR
                     QLFDLYVCLRPCKAYPGNPLNFKEDLDLVVFRENTEDLYAGVEFSPVPAELSQTLAKL
                     SPSFAPFRDLRPDDYAISCKINTRKGSERIVRAAFEFARTHKRKKVTVVHKANVVRAS
                     DGVFLEEARKVAKAFPEIAMDEANVDAICMWLLKNPKNYDVLVAPNLYGDVISDLCAQ
                     MVGGLGFGCSGNIGMKLAVFEPTHGSAPKYAGQNKVNPIATILAAKMMLDWLGEGAKG
                     RAVEAAVAHVLMEGKVRTYDMGGAATCMEMGEAVARALPKEA"
     gene            9463..11259
                     /locus_tag="PLB01_06630"
     CDS             9463..11259
                     /locus_tag="PLB01_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQQ77013.1"
                     /translation="MPSRTIPELLLARVGATPDAPAFLHPDGKAWKTLTWRDASLRAR
                     EIACGLLASGLRPGQCVAIASSTRVEWALADLAIGCAGGATATIYPSSSDEDTEFILR
                     DSGAPIVFVENAALAKKVLSRRSALPDLRHVVVFDAPETPVEGASSIAALGALGRTWD
                     AAHAGGFDAALRAVKAEDLAAVIYTSGTTGRPKGVELPHDCWVFEAEAVETTQTIGFR
                     DVQFLWLPLAHVFGKMCLTLGVQMGFPTAIDGRLDRIGDNMAAVEPTFVCAVPRVFQQ
                     ARAKVIRKALAEGGAKAAIFKWALDVGLDVARRRQAGRAVGLLLDAKHRVADRLVFSK
                     VRARFGKRLKFFFSGSVALPRGLTDFFAASGIAIAEGYGLTESTAMTFFNRPWANRFG
                     TVGTVVPGMDVRIAEDGEVLLRGRGVMRGYRNLPEETAAVIDADGWLHTGDIGTLDAD
                     GYLSITDRKKDLVKTSSGKYVAPQRVEGRLTTETPLIAQAVFHGEGRNYGTALLALSE
                     EEVRAWAAKNDLEDRAHEDLVKDERLLVLVRRHVDKVNEGLAPHEAVRKFAVLPRELS
                     VADGELTPSMKVRRKVVEERYRDVLDGLYVDR"
     gene            complement(11244..13400)
                     /locus_tag="PLB01_06635"
     CDS             complement(11244..13400)
                     /locus_tag="PLB01_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77014.1"
                     /translation="MAVALHTGRTVSHYEVGELLGSGGMGDVYRATDLRLGRRVALKF
                     LRPLADAELRRRILHEAQAASSLDHPNICTIFEVDEVGEDVFIAMAFYEGETLDRILA
                     RGPLSPERALGLGAQAARGLAAAHEELIVHRDVKPANLMVTRGDTVKILDFGIARILG
                     DPRATDHGTVVGTPAYMSPEQLRDEPVDPRTDVWSLGAVLYEMLTGTPPFRGGTVAAV
                     VASILGAPPPPPSSLHPGVPYRADEVLARALAKDRRLRYERMEDLARDLLELRSALDS
                     GAITLRRAAVAAVSSIAVLPFADMSPGQDQRFLCDGIAEEILRALARIPDLYVASRTS
                     AFQYRNQDADVREIGARLNVGAVLEGSVRRAGDRVRISAQLVSVENGFRLWNERYDRE
                     MKDIFAVEDEIAEKIAQALKVTLVERRENPAGRASVAAEAYELFLQGRQFFHQHRRKA
                     LEIAIQTFARAVELDPGYARAYAGMADCHSFLKLYFGRGEEAVAAADAASAKALDLEP
                     DLPDAHASRGLALFLQRKFPEAENFLRKAIALDPRVYDPHYIYGRVCFSEGRMPEAAG
                     HFREAAALVPEAYDAWYLLGMCHRRMREPAKARNADLECIEAAKKRLRWHPDDTRAWT
                     MGASVLAELGEPERAAEWLARALAVDRDEPIIQYNAACVYVALHRHDEAIACLEASAA
                     QGTLSADWLANDPDLDPLRDDPRFAALPGALVYRST"
     gene            13544..15541
                     /locus_tag="PLB01_06640"
     CDS             13544..15541
                     /locus_tag="PLB01_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQQ77015.1"
                     /translation="MDAPDSKSLPENAYEPLKPGETYAPVVPAIQSPPETTGRSVIWG
                     LFFCVVFTVASAYSGLKVGQVMEAAIPISILAIGLARVYTRRSHLLENVIITTIGGVA
                     GGIVAGAIFTLPALYTLKLHPHPAQTIFICLAGGCLGILFIIPLRRYFVKEMHGQFPF
                     PEATAITEVLVTGEKGGAQAKLLIQATVISGIYDFFVTTFQVWKEYVDFQFLPFMKDA
                     ADKAKVIVKFDAVAFILGLGYVMGLRASLILCAGGVLANFVLVPLIWMLGRHIPEAVY
                     PALKPIAQMSAEQIYRGYVRYVGVGAIATAGIFGILKSLKIVAGSFGIAAKAFRSGAG
                     VSSERTDRDISLIAILLGTILSAVAVGAFFGTLGSTLTAVLIALALALGFSFFFASVA
                     ANAIATIARNPVSGMTMLTIIISSVILLKFGVSGTTGMFFVMALAGMVCTALSVSGQM
                     ITDLKTGYWLGSTPAAQERLKFWGVLASAIAVGITIVLLAKVYQFGEVAPGDTREVLA
                     APQASIMKVLVETFMNRQPIAWFLFGTGAAIAVIMEMLRVPALIFALGMYLPLELNSP
                     ALVGGMISHFVLRKSEKAGGAAGRTMRERAVVIASGLMAGGALGGVFGAALRLFGWYR
                     EDLVRTPFYGDDAISQGVSIAFFVGLCLYLWIDSTRKVKEI"
     gene            15544..16899
                     /locus_tag="PLB01_06645"
     CDS             15544..16899
                     /locus_tag="PLB01_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77016.1"
                     /translation="MPSSSLTGLDAATAEALIGIDTLWGGDVMNPSGTGRFIADCWFS
                     DEPLPAAYTHPSAATLRANGGVGAKTPDKASIEAYVAAVDVKGAIARVVEGGRNAGGP
                     RGAYLEGLGLCSEVMWDLGMEILGKGPEVPYARCVETILGRGPAPSRPEEKRRRVADL
                     LRSAGHASNGGDSLLAAVDAWRHARLVPGKSIPALGDAFIAHYDALTAKNLLPFLPKE
                     FVRVPRANVKFLAIENAWFSGSMNYLGHARGADGSPEYEATYELNASLQISVPEFQQL
                     VSHEVVPGHVTTFAYLQDLFVRGLVGFEASVLTMNTRAAVLFEGIANNGVLVAHGVKE
                     PHELPDRDLEIGLLLALLQDDAKNHSSYLTWKEKLPQGEVAKTLRNEFLVSAERADKL
                     SGAWGRHPLLGRMYLPAYRAGTELVADLRRRHAPEKVLPALYGVNGLVDATTLPRMLE
                     G"
     gene            complement(16905..>17820)
                     /locus_tag="PLB01_06650"
     CDS             complement(16905..>17820)
                     /locus_tag="PLB01_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQQ77017.1"
                     /translation="RAGIPGGAIAGESRRDAPRPGLPRPAASGLARVWKSAPLGLRSV
                     FAALIGLTALSVFVFSWGMDLSLVDAFYFVVTTVTTVGYGDITPLKASPALKLFGCLL
                     MVLGSATLATLTSILTAFVVTERFERLLGRGRVPASGHVVVVGDGNVGSRTLGELARA
                     GVPAAAVDVDPAVRVRSAGAAVVVGDARRLEVLEEANVATARAVVTVTGDDAVNLGVA
                     LLARKLNPTIRTVVRLFDAGLAEKVQDGLDVDVAMGAARVAAPMFVAAILEEGVEAAV
                     VSGGALHVLRRGDSGLAWSRRPLAVPAA"
BASE COUNT         2618 a         6329 c         6196 g         2677 t
ORIGIN      
        1 cgccgctcgt agtcgaagcc gtcgaacgtc ggcttgaacg tcgccgggat ctcgctcggg
       61 tagtccgggt acttcggcgg cgcgggcgtc gccgctgcgg gcgcctgcgc ccgcgcggcg
      121 gacgccgcga gaacggcaga agaaagaaga agagaagaga agattttctt ggaaaggaag
      181 agcacgggag acctccgagc gcaacttgag acggcgaatc atggcacggc ccgcggcgta
      241 gactcgactt ccgccgtgag gccccctccg tttcgctcga tcccggatct cttcttcgcc
      301 cgcgtggagg cgacgccgga cgcgccggcg ttcctccatc ccgaggacgg ggcgtggaag
      361 agcctgtcgt ggcgggacac ggcgtcccgg gtgcgcgagc tcgcctgcgg gctcctctct
      421 ctcggcatct ccgccgggga tcgggtggcg atcgcgtcgt ccacccgcat cgagtggatc
      481 ctcgcggacc tcgccgtggc gtgtgcgggc ggcgtcacgg cgaccgtcta tcccgtgtcg
      541 accgaggacg acacgtcgca catcctgtcc gactcgcgcg cggcgatcgc ctttgccgaa
      601 gacgacgcgc aggcccggcg gatcctgagc cgccggggtg ggctcccctt cctgcgcaac
      661 ctcgtcctgt tcgacgcgtc ggcggggctt ccgggcgacg cgatgtccct ccgggacctc
      721 gcggcgctcg gccgcgagtg ggacgcggcc caccccggcg ggtacgaggc cgccgcggga
      781 gccgccggcc cctccgatct cgcgacgctg atctacacgt ccgggaccac cggccgtccg
      841 aaaggcgtcg agctgccgca cgactgctgg ctcttcgaga tcgagtcgat cggttcgctc
      901 ggactcctcg gcccatcgga cctccagttc ctgtggcttc ccctgtctca cgtcttcggg
      961 aaggtgtgcg tgacgctggg aatcgccttc ggcttcccca cggccgtcga cggccgcgtc
     1021 gaccggatcg cggcgaacct ctcgtccgtc aggccgacgt tcgtttgcgt cgtcccgcga
     1081 atcttcgaac gggtgcacgc gaagatcctc gagcaggcgc gcgcgggcgg aaagacgaag
     1141 gccgcgatct tcgactgggc cctgtcggtc ggcgtcgacg cggcgaggcg ccgcagcgag
     1201 ggccggcgcg tgggcgcgtt cctcggggcg aagcaccggc tcgcggacgc gctcgtcttc
     1261 cggaaggtcc ggcagcgctt cggcgggcgc ctccgcttct tcatctgcgg cagcgtcgcg
     1321 ctcgcgcgcg agctcaccga gttcttcgcc gcgttcggcg tcacgatcct cgagggctac
     1381 ggcctctccg agtcgacggc gatgacggtc tccaacctcc cggatcgcaa tcgctggggc
     1441 acggtcggga tcccgatccc cggcatcgag gtctctttcg cgggggacgg cgagatcctg
     1501 cttcgcggac gcgggatcat gcgcggctac ttcggcctgc cggaggagac ggcccgtgcg
     1561 ctcgacgtgc acggctggct ccacacgggc gacgtcggga ccctcgacgc ggccggccac
     1621 ctcgtgatca ccgatcggaa gaaggacctg atcaagacct cggggggaaa gttcgtcgcg
     1681 ccccagcaca tcgagggaat gctgaagctc gagacgccgc tcatcgccca ggtcctcctg
     1741 cacggcgagg gccgccgctt ctgcacggcg ctcatcgcgc tctcggagga ggagctcctc
     1801 gcgtgggcgc gcgccgaggg gatcgacgcg gcctacaagg acctcgtgca ggatccccgg
     1861 gtcgtcgagc tcgtgcggcg gcacgtcgaa cacgtgaacg cgcgcctggc gcgccacgag
     1921 gcgatccgac gctgggcgat cctgcccgga gagctgaagg tcgagaccgg cgacctgacg
     1981 ccgagcctga aggtgcgccg ccgggtcgtc gaacagaagt tccgcgacgt cctcgagggc
     2041 ctgtacgtcg agccgccgcc gcccgaagcc ccctaggagt caccgccgcc cggcgaagga
     2101 cgcgcgccgt tacactcgag cccgccatgc gcccgcgcgc gaccgccgcc cccctcctcc
     2161 tttcatggac tctcctcgtc ttcccttctc ctctcacggc ggcgcccgca gccgcgccga
     2221 agccttcccg caaaccgctc aagggctgca agtgggagaa gctcacggac gcggcggccg
     2281 gcctcgaggt gtgggcccag cgctgcgact tcggcttccg gaagatcgac ttcctgttcg
     2341 agaagaacgc gctcctgatc cgttactcgg acggcggcaa gccggatccc gtgatcgagc
     2401 ttctcgacct gaaagacggc gaagcggtcg aggcgggaat ccagaggctc ttttccgagc
     2461 ggaccagccg gttcctggtc tcgcgctgcc ggctcgtccc gtccgccggc aagcccccgg
     2521 ccggctcgaa gtggttcacg ttcgagccgg agccggcgta cgccgccgag ctccggaaga
     2581 agcaggaccc gaacgaggtc cccccgccgc cctgcgggga ctggggctcg gcgacggacg
     2641 gagtccagta ctgggaggcg cagccggcga gcggcgcgag gaaggtcctc ttcgtgaggg
     2701 tcgggcagga gacgccgctc tacgacgagc ggacgctcag gcttctgccg gcagcgccgg
     2761 cacggacggc accgccggca cgctgacggt cgcctccgcg aagacgacag ggacaccgcc
     2821 gcggtcggcc ctcacgacac gcgaccagag cgagatcgac gggtcgaaga ggaattggaa
     2881 caggagtttc gtccacgagc ggtgccagcg gagcgtgtcg tatgcctcgg gcgcggcgcg
     2941 ccggaccgac ggcagccgat gccacggcac ccacgggaag tcgtgatgct cgttgtggta
     3001 tccgacgttg agcgccggga cgttcaggaa cccgtagtaa ctcgaggttt cctggttcgt
     3061 gccgtgaacg aggtagtgct cctggatcca ccgggccccg agcggatgga ggccgaccga
     3121 gaagaagaag gccgcggcga ggaagagcag cgccttcggc ccgaggacgt acaggacggc
     3181 ggcgtcgtac gcgagctgga cgacgatgtt cacgaccacc cagccgtcga agagggagat
     3241 ctccttgagg cgcggcggcc gcgacagctg gaagaacggg aagaacagga gccagagcgc
     3301 cttccggagc gcggagtgcc ccacgaggcg cgcctcccag cggttcggga ggtctgcgtc
     3361 cagctcgtac acgccctgaa aggcgtggtg cttgaggtgg tacttctgga acgacacggc
     3421 gctcgggaac aggtgcggca ggttcgcggc catcgcgaag accttgttcc acgggccgct
     3481 cttcacgacg aggttgtggg cgcagtcgtg gatgaggacc cagagcgcgt ggctcgcgaa
     3541 cgcgccgacg agccacgcgg cggcgaggat gacccagacg ggctgcccgg agaggagtcc
     3601 ggcggccgcc gtctggaaaa cgacgagcgc ggcgatcagg acggcgctcc acgggttgcg
     3661 gcccgcgagc ttccggatgt cggcgtgcgc cttcaggatc tccttcgcgc ggaccgcatg
     3721 aggctcgcgg ccctgtgcga agacgaatgt cgagggcgcc gcagccggcg ccggggaaga
     3781 aaccgggctg ctcatggggc ccccttctcg agcctcagct tatcccgcga cccggtcccg
     3841 cgccacgagg gccccgaagt ccgcatccgg accgcccggc gcgcctctat acttcgggcc
     3901 aaatccggaa gtcggcgttc accgagcgag gcgcgatgca ggtcgctcca ggctcccgtc
     3961 tcggaccgta cgaggtcgtg gcccccgtcg gggcgggcgg gatgggaacc gtctggcgcg
     4021 cgcgcgacat gcgactcgac cgggacgtcg cgatcaaggt cgtccggccc gacctcctct
     4081 cggacgagat ggcgaggcgc cggttccgca aggaagcgct cgcgctcgcg aagctccagc
     4141 acgcgcacat cgcgtacatc ctcgacgtcg tgaccgaggg aagcgtcgac gcgctcgtca
     4201 tggagctcgt gccgggcgtc tcgctttccg agcgcctcgc gaagggcccg ctcggcgagg
     4261 ccgaggccgt ccgcctcggg atccagctcg cggacgcgct cgcggcggcg cacgcgcagg
     4321 gagtcgtcca ctgcgacctt aagcccgcga acctccgcgt cacgcccgac ggtgacctca
     4381 aggtcctcga tttcggcctc gcgaagctca gacgtccggc gctcggcggc gacctcacgg
     4441 cggccgcgac ggagtcggct acggctctcg aggccgtcgg gacgctgccg tacatggccc
     4501 ccgagcagct gagggccgag cccgtggacg cgcggacgga cgtctggggc gccggtgccg
     4561 ttctctgcga gctcgcaacc ggccggcggc cgttcgagga gaacgtcccg gcgttgctga
     4621 cggacgcgat cctgcacgcg gagcccccga ctctctcggg ttccggcccg gctccgtcac
     4681 ccggccttcg ggagattctt caaaagtgtc ttcggaagga gccgtccgag cgctacgaga
     4741 cggcggcgcg gcttcgcgcc gacctcgatt ccctttcgaa gaagccggcg gaagccgcgg
     4801 ctgcttccgc gcgttcttcg gcgtccactc cggcgtggcc ccgccggcgc atcgccgtca
     4861 cgggcggcct tctcgtcctc gccgccgtcg ccttcggcgc gctctggcgg tccggagtct
     4921 tcccgcgctc cgcggcgggc gggcggatcg actcgctggc ggtcctcccg ctctcgaacc
     4981 tctcgaggaa cccggagcag gagtacttct ccgacgggat gacggaggcc ctcatcgccg
     5041 agctctcgaa gatccgcgcg ctcaaggtca tctcacgcac ctccgtcatg cagtacaaga
     5101 acgcgacgaa gccgctcccc gagatcgcga aggcgctcgg ggtcaagggc gtcgtcgagg
     5161 gctccgtcat gcgcgagggc gagagcgtgc gaatcaccgt ccagctcatc gaggccgaca
     5221 cggaccggca cctgtgggcc gagagctaca cgcgcgacct caagaacgtc ctctcgctcc
     5281 agagcgaggt cgcgtcggcc atcgcgcgcg cggtgcgcgt cgccgtcacg ccggaggaaa
     5341 cgcgacggct ctccgcgagg aggaccgtgg atcccgaggc tcaccggctc gtcctcctcg
     5401 gccagcaggc cgtgcgggcg tccgcgtccc gcgactcatt cgagaaggcg gccggctact
     5461 accggcgcgc catcgagaag gacccggacg acgcgagcgc ccacctcagc ctcggcgcgg
     5521 ctctcttctc gatcggcgga ttcggctggc agccggtgaa gaccgcgtgc ggcgcggcgc
     5581 gcgccgaggc cgacaaggcc gccgcgctcg acccggactc cggggaggtc ctcggcttcc
     5641 gcgcctacct gcgctacttc tgcgaccgcg acacgcccgg cgccgtcgag gggctccggc
     5701 gtgccctcga cctcgcgccg gggtcggccg tcctgcacga ccagctggcg tggatcctct
     5761 cgagcgccgg ccgtcacgac gaggcgatcg ccgagtcggc gatcgcggtc gagctggatc
     5821 cgctccacga ccagatcacg gtccaccgcg gcatgtactt cggccaggcg aggcgccacg
     5881 cggaatccga gcgccagttc cgaaacgtcc tcgcgacgac gcccgactcc tcgttcgcga
     5941 agttcgcgct cggacaagag ctcatcgccc aagggcgcta cgacgaagcc ctcaccacgc
     6001 tcctctcgcg caaggtgccg cgccccgaga tgaacttcct cgtcggcctc gcgcaggggc
     6061 ttgccggacg gaaggacgag gcccggaagg tcctcgactt cctcctcgag aggcggagga
     6121 cccagttcgt cccgcccgcg atgatcgcga tggtctatct cggcctcggc gacaaggaca
     6181 aggccttcga atggctggat gccgcctgcg acgaacacgg catcttcacg gaccaggcca
     6241 aggtccaccc gatgttcgac ccgctccgcg ccgacccgcg cttcgacgcg ctcctccgga
     6301 agatgagcgc cccggcgtga cgccggagac gctcagcggg ctgcggctcc ccgcgccgcc
     6361 acgagccgcg cccgcgtgaa ggcgatgagc ccgcccgcgt cgatgatcgc ctgccgggcg
     6421 aacgggagcg gcacgacgtc gtaggccgtg cgcttcgtcc ggttctcgac cccgccgcgc
     6481 tcgaaaacga cctcgtcgcc ctcgtccgcg gtgaagccgg gcgcgacgac gacgttcaag
     6541 ccgaggttga tcgcgttctg caggaagatg cgggcgaagc tccgcgcgac gaccacgacc
     6601 tcgtgcccct tcaggcacga cgcggcctgc tcgcgcgacg agccgcagcc gaagttctcc
     6661 cctcccgcga tcacggcgcc cttcgggatc gccttcgcct tgacgagggc ctggaacgcg
     6721 gcgtggtccg cgaacgccag cgccggcgtc tccgacggga ggacggtcgc catgaagcgg
     6781 ccggggtaga tgacgtcggt cgacacgtcg tcgccgaggc tcaggatcac gcgggccgac
     6841 tcgaccttcg tgtcgggctt ccggctccgg gacttttcgg tctttcggac gcgtgccatc
     6901 tcagcgctcc ttccgcgggt cggtgatgac gcccgtgagg gccgacgccg ccgcgacggc
     6961 aggggacgag aggtagacct ccgcgcccgg gtttcccatc cggcccttga agttccggtt
     7021 cgtcgtcgag atcgccgtct ccccgtcgcc gagcgcgccc tcgtggacgc cgaggcacgg
     7081 accgcagccc gcgttcatga cgacggcgcc ggcggccatg aaatcggaga ggtagccgag
     7141 cttcatcgcc tcggcccaga tcttcccgga cgccgggaag acgagcatgc gcgtcccggc
     7201 ggccacgtgc ctgcccttca cgatcgccgc ggcctcggcg aggtcgtcca gccgcccgtt
     7261 cgtgcacgag ccgatcacga cctgctgcac gggcttcccc gccaccgcgg acacgggctt
     7321 gacgttgtcc acggtgtgcg ggcaggcgac gaggggctcg agtttcgaga cgtcgaccga
     7381 gaccgtcttc tcgtacgtgg cgtcgggatc gctcgtgacg aggtcgatcg ggtccgtgac
     7441 gccgacgtcc tcgcgaaggt accgcacggt ctccgcgtcg cccggcacga tgccggcggt
     7501 cgcgcccgcc tcgaccgaca tgttgcagag cacgagccgc cccgacgtcg agaggttcgc
     7561 gatcgtctcg cccgtgaact cgaggacgcg gaagtttgcg ccctgcgccg tgagatggcc
     7621 gatgagatgg aggatgaggt ccttcggccc gacgtgcttc ccgaatcggc cggacacctc
     7681 gacgcggatc gtcgggggaa cctcgatgtt cagggcgacg ccgagcgccc acgcgctcgc
     7741 catctccgtg gcgccgatcc cgaatgcgaa cgcgccgagc gccccgtggc tcgtcgtgtg
     7801 ggagtccgta ccgacgacga ccatcccggg ccgcacgtag ccgcgctcgg ggaggatctg
     7861 gtggcagatt ccgcccacgt cgccgcgcac gtcgtggaac ttctcgattc cgtgctcggc
     7921 gacgaaggcg cggaccttct tctggttcgt cgccgtcttc gccgactcgg ccgggacgcg
     7981 gtggtcgaaa atgatcgcga tccgcgaagg atcccagggc ttggcggtca gcccggtccc
     8041 ctcgtagatc tccctgaact ggttgatgac gagcgccgcg ttctcgtgcg acatcgcgag
     8101 gtcgacacgc ggctcgacga cgtcgccggc cctgacggcg ggtttgcccg aggcgcgggc
     8161 caggatcttc tcgacggcgg tggcgcccac gttcaggcct ccttcggcag cgcgcgggcg
     8221 acggcctcgc ccatttccat gcacgtggcg gcgccgccca tgtcgtacgt gcggaccttt
     8281 ccttccatga ggacgtgggc gacggccgcc tcgacggcgc gtcccttcgc gccctcgccg
     8341 agccagtcga gcatcatctt cgccgcgagg atggtcgcga tcgggttgac cttgttctga
     8401 cccgcgtact tcggggccga gccgtgcgtc ggctcgaaca cggcgagctt catgccgatg
     8461 ttcccggagc agccgaatcc gagcccgccg accatctgcg cgcagaggtc cgagatgacg
     8521 tcgccgtaga ggttcggggc gacgaggacg tcgtagttct tcgggttctt caggagccac
     8581 atgcagatcg cgtcgacgtt cgcctcgtcc atcgcgatct cggggaacgc cttcgcgacc
     8641 ttgcgcgcct cctcgaggaa gacgccgtcg gaggcgcgca cgacgttcgc cttgtggacg
     8701 accgtgacct tcttccgctt gtgcgtgcgc gcgaactcga acgcggcgcg gacgatgcgc
     8761 tcggacccct ttcgcgtgtt gatcttgcag gagatcgcgt agtcgtccgg acggaggtcc
     8821 ctgaagggcg cgaaggacgg cgacagcttc gcgagggtct gcgagagctc cgccggcacc
     8881 ggcgagaact cgacgcccgc gtagaggtcc tcggtgttct cgcggaaaac gacgagatcg
     8941 aggtcctcct tgaagttgag cgggttgccc gggtaggcct tgcacggcct caggcacacg
     9001 tagaggtcga acagctgccg catccggacg atcggcgagc ggtacacgag tccctgggtc
     9061 ctcagggagg gcgcgagctc gtcgagggcg gccttcgacg gcttggaggt gatcgcgccg
     9121 aacatcgcgg cgtcgacgtt cttgagcagc tcgatcgtgc gggcggggaa cgcatcccct
     9181 tccgcgcacc agaattccca gccgatgtcg ccgtgcgggt actcggcgtc gaggcccacg
     9241 gcgtccagga ccttgcgggt tgcatccatg acttccacgc ccacgccgtc cccgggaagc
     9301 cacgcgatcc ggtaacgaga catagatacc tctccatccg aaaagtacct cctggagggc
     9361 gcggggcgga ggaggcaggt gcatcagggc gccgcatgaa gctcctgaat cagggcttag
     9421 cgccgttccc ggacgcagga ttggagcctg gagggctcta ccttgccttc acggacgatc
     9481 cccgagctcc ttctcgccag agtcggcgcg acacccgacg cccccgcgtt ccttcacccc
     9541 gacgggaaag cctggaagac gctcacgtgg cgcgacgcct ctctccgcgc cagggaaatc
     9601 gcatgcgggc tgctcgcctc gggcctcagg cccggacaat gcgtcgcgat cgcgtcgtcg
     9661 acgcgcgtcg agtgggcgct cgcggacctc gcgatcggct gcgcgggcgg cgcgacggcg
     9721 acgatctacc cctcgtcatc ggacgaagac acggaattca tcctccggga ctcgggggcg
     9781 ccgatcgtgt tcgtcgagaa cgcggcgctc gcgaagaagg tcctgagccg ccgcagcgcg
     9841 ctccccgacc tccggcatgt cgtcgtcttc gacgcgcccg aaaccccggt ggagggcgcc
     9901 tcgtcgatcg ccgcgctcgg cgcgctcggg cgcacgtggg atgccgcgca cgcgggcggc
     9961 ttcgacgcgg ccctccgcgc cgtcaaggcg gaggacctcg ccgcggtgat ctacacctcg
    10021 ggcaccacgg gccggccgaa gggcgtcgag ctgccccacg actgctgggt cttcgaggcg
    10081 gaagccgtgg agacgacgca gacgatcgga ttccgcgacg tgcagttcct ctggctcccg
    10141 ctcgcgcacg tcttcggcaa gatgtgcctg acgctcggcg tccagatggg cttcccgacg
    10201 gcgatcgacg gacggctgga ccggatcggc gacaacatgg cggcggtcga gcccacgttc
    10261 gtctgcgcgg tgccgcgcgt cttccagcag gcgcgcgcga aggtcatccg caaggcgctg
    10321 gccgagggcg gcgcgaaggc ggccatcttc aagtgggcgc tcgacgtcgg gctggacgtc
    10381 gcccggcgcc ggcaggccgg gcgcgcggtg gggctgctcc tcgacgcgaa gcaccgcgtc
    10441 gcggaccgtc tcgtcttctc caaggtccgc gcgcgcttcg gcaagcgtct caagttcttc
    10501 ttcagcggca gcgtcgcgct gccgcggggg ctcacggact tcttcgcggc gtcgggcatc
    10561 gcgatcgccg agggctacgg cctcacggag tcgaccgcga tgacgttctt caaccggccg
    10621 tgggcgaacc gcttcgggac cgtcgggacg gtcgtgcccg gcatggacgt gaggatcgcc
    10681 gaggacggcg aggtcctgct gcgcgggcgc ggggtcatgc gcggctaccg gaacctcccc
    10741 gaggagacgg ctgccgtgat cgacgcggac gggtggctcc acacgggcga catcgggacg
    10801 ctggacgcgg acggatacct ctcgatcacc gaccgcaaga aggacctcgt caagacgtcg
    10861 agcggcaagt acgtcgcgcc gcagcgcgtc gaggggcggc tcacgacgga gactccgctc
    10921 atcgcgcagg cggtcttcca cggcgagggg cgcaactacg ggaccgctct cctcgcgctc
    10981 tcggaggagg aggtccgcgc ctgggcggcc aagaacgacc tcgaggaccg cgcgcacgag
    11041 gacctcgtga aggacgagcg ccttctcgtg ctcgtgcgcc ggcacgtgga caaggtgaac
    11101 gagggcctcg cgccgcacga ggccgtgcgg aagttcgcgg tgctgccgcg cgagctctcc
    11161 gtcgcggacg gcgagctgac gccgagcatg aaggtccgcc ggaaggtcgt cgaggagcgc
    11221 taccgcgacg ttctcgacgg gctctacgtc gaccggtaga ccagcgcgcc gggcagggcc
    11281 gcgaagcgcg ggtcgtctcg caacgggtcg aggtcgggat cgttcgcgag ccagtcggcg
    11341 gaaagggtgc cctgcgcggc cgaggcctcg agacacgcga tcgcctcgtc gtgccggtgc
    11401 agcgccacgt acacgcatgc cgcgttgtac tggatgatcg gttcgtcacg gtccacggcg
    11461 agagcgcgcg caagccactc ggccgcgcgc tcgggctcgc ccagctccgc gagcacggag
    11521 gcgcccatcg tccaggcgcg cgtgtcgtcg gggtgccacc ggaggcgctt cttggccgcc
    11581 tcgatgcact cgaggtccgc gttccgcgcc ttcgcgggct cgcgcatgcg ccggtggcac
    11641 atcccgagga gataccaggc gtcgtaggcc tcgggcacga gggccgccgc ctcgcggaag
    11701 tggcctgccg cctccggcat gcgcccctcc gagaagcaca cgcggccgta gatgtagtgc
    11761 gggtcgtaga cacgggggtc cagcgcgatc gccttccgga ggaagttttc cgcctccggg
    11821 aacttccgtt ggaggaagag cgcgaggccg cgcgaggcgt gggcgtcggg gagatcgggc
    11881 tcgaggtcca gtgccttcgc gctcgcggcg tcggccgccg cgaccgcctc ctcgcccctt
    11941 ccgaagtaga gcttgaggaa cgaatggcag tcggccatgc ccgcgtaggc gcgggcgtat
    12001 ccggggtcca gctcgaccgc ccgcgcgaac gtctggatcg caatctccag cgccttgcgc
    12061 cggtgctggt ggaagaactg ccgcccctgc aggaagagct cgtaggcctc ggcagccacg
    12121 gacgcgcgtc ccgccgggtt ctcccgccgc tcgacgagcg tgaccttgag agcctgcgcg
    12181 atcttctcgg cgatctcgtc ctcgaccgcg aagatgtcct tcatctcccg gtcgtagcgc
    12241 tcgttccaga ggcggaaccc gttctcgacg ctcacgagct gcgcggagat ccgcacgcgg
    12301 tcgccggcac ggcggacgct tccttcgagc accgcgccga cgttcaggcg ggcgccgatc
    12361 tcgcgcacgt cggcgtcctg gttccggtac tggaacgcgg acgtccgcga cgcgacgtac
    12421 aggtcgggga tgcgcgcgag cgcgcgcagg atctcctccg cgatgccgtc gcagaggaac
    12481 ctctggtcct gcccggggct catgtcggcg aaggggagga cggcgatgga cgacacggcc
    12541 gcgacggcgg cgcggcgcag cgtgatcgct ccggagtcga gcgcggagcg gagctcgagg
    12601 aggtcgcgcg ccaggtcctc catccgctcg taacgcaggc gcctgtcctt cgcgagcgcc
    12661 cgcgcgagga cctcgtcggc gcggtacggg actccgggat gcaggctcga cggggggggc
    12721 gggggcgcgc ccaggatgga cgccacgacg gccgccaccg tcccgccgcg gaacggcggc
    12781 gtgcccgtga gcatctcgta caggacggcg ccgagggacc agacgtcggt tctcgggtcc
    12841 acgggctcgt cgcgcagctg ctcgggagac atgtatgcgg gcgtgccgac gacggttccg
    12901 tggtcggtgg cgcgggggtc gccgaggatc cgggcgatcc cgaagtcgag gatcttcacg
    12961 gtgtcgccgc gggtgaccat gaggtttgcg ggcttcacgt cgcggtggac gatgagctcc
    13021 tcgtgcgcgg ccgcgagacc gcgcgccgcc tgcgccccga gccccagcgc ccgctcgggc
    13081 gaaagcggtc cacgcgcgag gatgcggtcg agcgtctcgc cctcgtagaa cgccatcgcg
    13141 atgaagacgt cctcgccgac ctcgtccacc tcgaagatcg tgcagatgtt cgggtggtcc
    13201 agcgacgagg cggcctgcgc ctcgtgcagg atccgccgcc gcagctccgc gtcggcgagg
    13261 ggccgcagga acttcagcgc gacgcgtcgc ccgaggcgca ggtccgtcgc ccggtagacg
    13321 tctcccatgc ctcccgagcc gagtaattcc ccgacctcgt agtgcgagac ggtccgtccc
    13381 gtgtgaaggg ccaccgccat gcccgccaag atagggcccc ggcagcccgg cggccatgcc
    13441 tccgccctcc tccctgatac acctgactca gttcctccgc accgccctcc ttcttacgct
    13501 cacgctggca aggtctgcca agatcctgcg caccggagga ccgatggacg cacccgactc
    13561 gaagtcgctg cccgagaacg cctacgagcc gctcaagccc ggcgagacct acgctccggt
    13621 cgtcccggcg atccagtctc cgcccgagac gaccggacgc tcggtgatct ggggcctttt
    13681 cttctgcgtc gtcttcaccg tggcctcggc gtactcgggc ctcaaggtcg gccaggtcat
    13741 ggaggccgcc atcccgatct cgatcctcgc gatcggcctc gcgcgcgtgt acacccggag
    13801 gtcgcacctc ctcgagaacg tcatcatcac gacgatcggc ggcgtcgcgg gcggcatcgt
    13861 cgcgggcgcg atcttcacgc tccccgcgct ctacacgctc aagctccacc cccaccccgc
    13921 ccagacgatc ttcatctgcc tcgcgggcgg gtgcctcggg atcctcttca tcattccgct
    13981 gcgccgctac ttcgtgaagg agatgcacgg gcagttcccg ttcccggagg cgaccgcgat
    14041 cacggaggtg ctcgtcacgg gcgagaaggg cggcgcgcag gcgaaactcc tcatccaggc
    14101 gacggtcatc tccggcatct acgacttctt cgtcacgacc ttccaggtct ggaaggaata
    14161 cgtcgacttc cagttccttc cgttcatgaa ggacgccgcc gacaaggcga aggtcatcgt
    14221 gaagttcgac gccgtcgcct tcatcctcgg cctcggctac gtcatgggtc tgcgggcctc
    14281 tctcatcctg tgcgccgggg gcgtgctcgc gaacttcgtc ctcgtgccgc tcatctggat
    14341 gctcggacgg cacattccgg aagcggtcta ccccgccctg aagcccatcg cccagatgtc
    14401 ggccgagcag atctaccggg gctacgtccg ctacgtcggc gtcggagcca tcgcgaccgc
    14461 cgggatcttc gggatcctga agtcgctcaa gatcgtggcg gggtcgttcg ggatcgcggc
    14521 gaaggcgttc cggagcggcg ccggcgtctc gtccgagcga accgaccgcg acatctcgct
    14581 cattgcgatc ctcctcggga cgatcctctc cgcggtggcc gtcggagcct tcttcgggac
    14641 gctgggctcg acgctcacgg cggtcctgat cgccctcgcc ctcgccctcg gcttctcgtt
    14701 cttcttcgcg tccgtcgccg cgaacgcgat cgcgacgatc gcccgcaacc cggtgtcggg
    14761 gatgacgatg ctcacgatca tcatctcgtc ggtcatcctc ctgaagttcg gcgtgtcggg
    14821 cacgacggga atgttcttcg tcatggccct cgccgggatg gtctgcacgg cgctctcggt
    14881 ctccggccag atgatcacgg acctgaagac cggctactgg ctgggctcga cgcccgccgc
    14941 gcaggagcgg ctgaagttct ggggcgtcct cgcctctgcg atcgccgtcg ggatcacgat
    15001 cgtcctcctc gcgaaggtct accagttcgg cgaggtggcc cccggagaca cgcgcgaggt
    15061 cctcgcggcg ccgcaggctt cgatcatgaa ggtgctcgtc gagacgttca tgaaccggca
    15121 gcccatcgcg tggttcctgt tcgggacggg cgccgcgatc gccgtcatca tggagatgct
    15181 ccgcgtgccc gccctcatct tcgcgctcgg gatgtatctc ccgctcgagc tgaactcgcc
    15241 ggcgctcgtg ggcgggatga tctcgcactt cgtcctgagg aaatccgaga aggcgggggg
    15301 ggcggccggg cgcacgatgc gcgaacgagc cgtcgtcatc gcctccggcc tcatggcggg
    15361 cggcgcgctc ggcggcgtct tcggcgctgc gctgcgtctc ttcgggtggt accgcgagga
    15421 cctcgtgagg acgccgttct acggagacga cgccatctcg caaggcgtct cgatcgcctt
    15481 cttcgtgggt ctctgcctct acctctggat cgactcgacc cgcaaggtga aggagatctg
    15541 aacatgccga gctcgtctct gactggactc gacgccgcga ccgccgaagc tctcatcgga
    15601 atcgacacgc tctggggcgg agacgtcatg aacccgtccg gcaccggccg cttcatcgcg
    15661 gactgctggt tctcggacga gccgctgccc gccgcctaca cccacccgtc ggccgcgacg
    15721 ctgcgcgcga acggcggcgt cggcgcgaag acccccgaca aggcctcgat cgaggcgtac
    15781 gtcgccgccg tggacgtgaa gggcgcgatc gcccgcgtcg tcgagggcgg gcgcaacgcc
    15841 ggcgggccgc gcggcgcgta cctcgagggc ctcggcctct gcagcgaggt catgtgggac
    15901 ctcgggatgg agatcctcgg caaagggccc gaggtgccgt acgcgcgctg cgtggagacg
    15961 atcctcggac gcgggcccgc gccctcgagg ccggaggaga agcggcggcg cgtcgcagac
    16021 ctcctgagat ccgccggcca cgcctcgaac ggcggggact ccctcctcgc ggccgtggac
    16081 gcgtggcgcc acgcgcgcct cgtccccggg aagtcgattc cggctctcgg agacgcgttc
    16141 atcgcgcact acgacgcgct caccgcgaag aacctcctcc cgttcctccc gaaggagttc
    16201 gtccgcgtcc cgcgcgcgaa cgtgaagttc ctcgcgatcg agaacgcgtg gttctcgggc
    16261 tcgatgaact acctcgggca cgcccgcggg gcggacggct cgcccgagta cgaggcgacg
    16321 tacgagctga acgcctcgct ccagatctcc gtgcccgagt tccagcagct cgtgagccac
    16381 gaggtcgtgc ccgggcacgt cacgacgttc gcctacctgc aggacctctt cgtgcgcggg
    16441 ctcgtcggct tcgaggcgtc cgtcctcacg atgaacacgc gcgccgccgt gctcttcgag
    16501 ggcatcgcga acaacggagt cctcgtcgcg cacggcgtga aggagccgca cgagcttccg
    16561 gaccgcgacc tcgagatcgg cctcctcctg gcgctcctgc aggacgacgc gaagaaccac
    16621 tcctcgtacc tgacgtggaa ggagaagctc ccgcagggcg aggtcgcaaa gaccctccgg
    16681 aacgagtttc tcgtctccgc cgagcgcgcg gacaagctct cgggcgcgtg gggccgccac
    16741 ccgctcctcg gacggatgta cctgcccgcc taccgcgcgg ggaccgagct cgtcgccgac
    16801 ctgcgccggc gccacgcgcc ggagaaggtc ctgcccgcgc tgtacggcgt gaacggcctc
    16861 gtggacgcga cgacgctgcc gcggatgctg gagggctgac cgggtcaggc cgcggggact
    16921 gcgagcggcc tgcggctcca cgcgagtccc gaatcccccc ggcgcagcac gtgcagcgcg
    16981 ccgccggaga cgacggccgc ttcgacgcct tcctccagga tcgcggcgac gaacatcggc
    17041 gcggcgacgc gcgcggcgcc catcgcgacg tcgacgtcca ggccgtcctg caccttttcg
    17101 gcgagcccgg cgtcgaagag gcggacgacc gtccggatcg tcggattcag ctttcgcgcg
    17161 aggagcgcaa cgccgaggtt gaccgcgtcg tcgcccgtga ccgtgaccac ggcccgcgcc
    17221 gtcgcgacgt tcgcctcctc caggacctcg agacgccgtg cgtcgccgac gacgaccgcc
    17281 gcgcccgccg accggacccg caccgccggg tccacgtcga cggcggccgc cggcacgccc
    17341 gcgcgcgcga gctctccgag ggtccgcgag ccgacgttgc cgtccccgac gacgaccacg
    17401 tgcccggatg cgggcacgcg gcctcgcccg aggagccttt cgaagcgctc cgtcacgacg
    17461 aaggccgtca ggatggacgt cagcgtcgcg agcgtcgccg agccgaggac catgaggaga
    17521 cagccgaaga gcttcagcgc cggcgacgct ttgagcggcg tgatgtcgcc gtagccgacc
    17581 gtcgtcacgg tcgtgacgac gaagtagaac gcatccacga gcgacaggtc catcccccac
    17641 gagaagacga agacggagag cgccgtgagg ccgatcagcg ccgcgaaaac cgaccggagg
    17701 ccgaggggcg cgctcttcca cacgcgcgcg aggccggacg cggcgggccg cgggagcccg
    17761 gggcggggcg cgtcccgccg cgactcgccc gcaattgcgc ctccaggaat ccccgcgcgt
//
LOCUS       DAOMFY010000055        17473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1024_length_17473_cov_6.380468, whole genome shotgun sequence.
ACCESSION   DAOMFY010000055 DAOMFY010000000
VERSION     DAOMFY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17473
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1024_length_17473_cov_6.380468"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /gene="recJ"
                     /locus_tag="PLB01_06655"
     CDS             <1..1544
                     /gene="recJ"
                     /locus_tag="PLB01_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQQ77018.1"
                     /translation="AVERLAATAARGGRVVVFGDYDCDGIGALAILTTALRKLGADAR
                     PFIPHRLHDGYGLRAPALRRALDEHEPEGIVTVDCGITAVETVAEATGRGVYVVVTDH
                     HLPPAALPEGAVLVDPKLPGCRYPFKELCGAGIAWKLSEALLIHSGPRVGIDAAARRR
                     WLASLAKIAALSTVADMVPLTGENRVLVSWGLSGLADPRSPGLSALLKRSGVPAGRAP
                     STREVAFRIAPRLNATGRIDHAARAFELLTTADAARAEALADEIEIANDERRAVQEKL
                     VETVLARLETTFDPARDAVVVEAGPASEGWHRGVLGIAASRVAQQVGRPVLLLSRDGE
                     TVGGSGRSFGRTPLYERVAPVAARYTKEFGGHAAALGLTIPAASWEAFREDLRAAFAA
                     ARDDAEWASDLEVDTEVEAPEADEALARALELFEPHGQENPKPLLLLRGLTWDGRGRP
                     VGEAGLRVSFASSGRRLDAVGWNLAEIPAPSRAGRWDVAANLAVDSFTGRPGLTVVDL
                     SPEAA"
     gene            1541..3556
                     /locus_tag="PLB01_06660"
     CDS             1541..3556
                     /locus_tag="PLB01_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HQQ77019.1"
                     /translation="MSRFARLARAAVLVLGVAFALLVVLSYRKPGARTEDAKDPVAET
                     LLAEANGARDRMRFRDFRYDETRESEGRYRVTASEAVRFDDQGNRLFRLKDVLFESRE
                     SRDGRTVSIRAPRAELAEGSRAFRIFDGVEVAGEEMRVTGASFRYDPARRLLASEGPV
                     DATRGGLVASADAGSVDTRDGVLVLDGDARLRGRGDEGRPVELRAPRVLVGRNGRLEA
                     SGGAVLKTDRFVLRGETFVREATPVGSHVRATTGARRLVPPDRGQPPAALAVAGDVLD
                     LALDAQGRPAAFEASAAGAARVDLAPTETTGARRAESPRFTGRFENGRLAELNVPEDL
                     RTAESARAGGPPGSGLRTFAAGFARLTFGSGLALETGTFEKDVAAADGTRASVKAPHA
                     TLRGRDETAVFSGEPGTPADYRDERGTIRARTLAWSRRDERVDAAGSVKASYAGGAGR
                     RVGLLGSESSAPFFTESDTLRLSGRASKVLLTGSVRAWQNENVLRCGTLELDDAEKTL
                     RAEQNVRAFFRRETTPAKGKAGKAAAPAGSETVNASSDVLTHREADRFVRLEGHATIT
                     SGPWTMNADVTDIRLSETRAIEYAEARGAVVVEDRAEHRRGEGTKAVWRPSTEAVTLE
                     GSPATALDGRGNKSTGAALTFRQGRSQVDVETGGNVPTETVLRPEGS"
     gene            3553..4290
                     /gene="lptB"
                     /locus_tag="PLB01_06665"
     CDS             3553..4290
                     /gene="lptB"
                     /locus_tag="PLB01_06665"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQQ77020.1"
                     /translation="MSDEKPLLEGRGLRKAYRGRTVVNDVSVRVEAGEVVGLLGPNGA
                     GKTTTFSMVVGLVTPDAGAVLLDGEDVTTLPMFRRARRGLGYLPQEPSIFRRMTAVEN
                     LLAILETLPGGRTEREETAHGLLDRFKLSHLAHSVADTLSGGERRRLEIARALTLSPR
                     FILLDEPFAGIDPITVLDLQQVIRDLATAGIGILITDHNVRDTLAIVNRGYIIHSGTI
                     FRTGSPETLSADPEVRRVYLGESFRLD"
     gene            4370..6061
                     /gene="rpoN"
                     /locus_tag="PLB01_06670"
     CDS             4370..6061
                     /gene="rpoN"
                     /locus_tag="PLB01_06670"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQQ77021.1"
                     /translation="MGLEQKLSLRLSQRLVMTPTLQQAIKLLQMTRLELQDVVNQELV
                     SNPVLDEEDADQAGGPDEEPGAAETRELDLASSADAPDAALEAAVADDREFAAAATDA
                     DARAESAEALPAPTSDVIAPEPVVDSSTGATDDPFSDLELEAYFGDYMEGTATAPRMN
                     EESEEFSLENRPESPPGLEPHLTEQLGVSDAPPDVREACGFLIGNVDPDGCLRVTLEE
                     TAEAVPCSIETAEKALALLQSFDPVGVGGRSLAEILLLQARAANVATPLLVELVTQRF
                     AELGSKPPALLARQMGVPVEDIQQALEWIRRLDPKPGRRYDSTRTIYVEPDVAVVKVE
                     DDYIVVFNDDGLPRLKVSALYRRMLLSRDGNLDSEGKSYLREKMRAAQWLLKSLDQRK
                     RTIVRVAESIVKKQRDFLDWGVAHLKPLVLRDVAEDIGMHESTVSRVVSNKWMATPRG
                     LVPMKFFFHSAIASTGGEDVSSLAVKNKIRGLIEAEDGAHPLSDARLAELLAHEGIQI
                     ARRTVAKYREELRIPASSIRRTGETAPAFERPEGAVDAAAEDAAADAEPSEQEEP"
     gene            6058..6591
                     /locus_tag="PLB01_06675"
     CDS             6058..6591
                     /locus_tag="PLB01_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQQ77022.1"
                     /translation="MKTDYTARHIRIHSGLREVVETKLDRLERVLPPRAVARVVVTAG
                     KMDVEVEVSVVGKTRTITAAARAADQRTAAQEAMERLAGQVRKTKAIVKEAKKHRASG
                     VRGAGTWAEPEPAPAPVPGPKREAVSPRAMFEEDALTHFAASKREFMVFRDAGSGHQI
                     RFLYRRRDGGFHLVVPE"
     gene            6594..7613
                     /gene="hprK"
                     /locus_tag="PLB01_06680"
     CDS             6594..7613
                     /gene="hprK"
                     /locus_tag="PLB01_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HQQ77023.1"
                     /translation="MRNPPEMRAPLVRSIDLRSPVLASLNLKLVAGHQGLQRPIDWPR
                     VQKPGLAIAGFLDYVKDNRVQILGASEFNYLKTLPPSVVRERLDAFTNLPLSAVVVTK
                     GIHPGPVLTGLCRRRHIPLFTTERLTSVVIEGLTAFLEESLAPRATLHGVLMEVGGLG
                     TLLLGDSGVGKSECALSLVQRGHRLVADDLVVVQRFPNDVLIGSSSGLIRHHMELRGL
                     GIVNVPMLFGVAAVLERHTVEFVIQLQDWKEGTEVDRLGLDEEFEEILGVAVPRVRMP
                     VATGRDVALLVEIAARNQLLKRRGFNAAREIAQKVHDEIVRKAAADHVARRVRRKFTA
                     RERER"
     gene            7610..8476
                     /gene="rapZ"
                     /locus_tag="PLB01_06685"
     CDS             7610..8476
                     /gene="rapZ"
                     /locus_tag="PLB01_06685"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQQ77024.1"
                     /translation="MSEGKRLVIATGLSGSGKSYVAHALEDIGYATVDNLPLSLLEDY
                     VGGVAADRTPSRRNAVVLDVRNPDFATRFPELLETLRARVPITVVFLDCEEKCLLNRF
                     SETRRPHPLGEGRSVAEAVAYERRLLAEVKAKADLVIDTTVMTVHELRAAVAQNFREP
                     GDPGVLAVSLVSFGFKHGLPAALDLCFDVRFLVNPHFDARLRPLTGRNADVARFIEAG
                     ELTEPYYRQLAGFLAFCLPNYRRENRAYLTIGIGCTGGRHRSVYVAERLARDLTAMGY
                     PVRVSHRDEALD"
     gene            8478..8921
                     /locus_tag="PLB01_06690"
     CDS             8478..8921
                     /locus_tag="PLB01_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQQ77025.1"
                     /translation="MTAPTGPAVGLILLSHGPLANALRETVHMLEPDEPDDIGALSLA
                     WDEATEAATRRLEKAIAAHDRGRGIVILTDMFGGTPSNLALAFLEPGKVEIVSGINLP
                     MVVKARALAREGKSAREIAHVLVERGRRAIVAAAELVDGAPKGTP"
     gene            8918..9187
                     /locus_tag="PLB01_06695"
     CDS             8918..9187
                     /locus_tag="PLB01_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQQ77026.1"
                     /translation="MKEEVLTLRNRLGLHARAAAKFVHAAASFESKITITKDGDEVDG
                     KSILGLLLLAAGKGAPLLVRAEGPDEDRALAALRDLVDRKFDEAE"
     gene            9209..10999
                     /gene="ptsP"
                     /locus_tag="PLB01_06700"
     CDS             9209..10999
                     /gene="ptsP"
                     /locus_tag="PLB01_06700"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQQ77027.1"
                     /translation="MKRPLSLTGIAVSPGVAVGRAVLWVSREDTSPRRTLEPEEIPDE
                     IERFRRAAAASVAEIEETARHVKERLGSEYAGIFHAHALFLQDKAFVGPVERRIVHDA
                     ANAEWAVAQTTEDLVARFRSLPDENLRDRASDLDDVSRVLRRHLGGEEQSRLKMSDLA
                     GEAIVLVADELTPSDAVRIPRDRVIAFVTERGGKTSHAAILARSFGLPAVVAVHGLLT
                     AVGDGDRVVVDGRDGVVWREPSEDIVSLFRDRRDSEARRELSLKERALSGPAHSADGT
                     PVEIRANIELTSEIPDVVGYGADGVGLFRSEFLYLSKEGVEFPTEEEQAAVYREVVSG
                     LAPRPVVIRTYDLGGKKGARHLVGAEEINPVLGLRGVRLCFARPEMFMTQLRALLAAA
                     NAGDLRILVPMVSSVEEFRRVRELVKRAREEVLAKGIAVPDDIPLGAMIEVPSAAITA
                     DLLAPETDFLALGTNDLIQYTLAVDRANETVSELFRPTHPAVLRLIAQVASAAQAARK
                     PLSVCGEMAADPALLLLFLGFGIREFSMGARSVPILKDFVRGVNVADAARIAKAALSL
                     STADEVSSLLAQELRSLPAGEPLRRLEVSA"
     gene            10996..11373
                     /locus_tag="PLB01_06705"
     CDS             10996..11373
                     /locus_tag="PLB01_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQQ77028.1"
                     /translation="MTLGTSRPRVVPKPWGEERIFAETGRYAGKLLVIRKGETLSYQY
                     HVRKEETIHVLDGVLGLQLEKDGEAADLRVPPGETFHVTPGTRHRMYAPEGDCLLVEV
                     STPELDDVVRLVDRYGRAGTSAP"
     gene            11370..12074
                     /locus_tag="PLB01_06710"
     CDS             11370..12074
                     /locus_tag="PLB01_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77029.1"
                     /translation="MMRRRVPGILAIVLLAAAGAEAQAPALSGLSPTPTPAAATSGEA
                     VPEATPAPKLKGVAGSATEAGRSGQSLADVVRVSKEARKDQKPKKSLGTITNESLHKG
                     GSTPAVTSKGPSKTPAASAVRKEPTPAPTYDVPRDDQGRSEAQWKATMDHARSLVQDG
                     ERRVKELETKSKQLENDFYAQSDGYRRDGVIKPAWDKSRDELAKARLDLDAARKSLED
                     LSEEARRSNAPPGWLR"
     gene            12096..13460
                     /gene="miaB"
                     /locus_tag="PLB01_06715"
     CDS             12096..13460
                     /gene="miaB"
                     /locus_tag="PLB01_06715"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQQ77030.1"
                     /translation="MSVNPHLKGGRRVFVETWGCQMNVLDGRRFAGLLARDGYDETFD
                     ESDADVFILNTCSVRDKAEQKVYDRVGRLAKLKRERPGLVVGVCGCVAQQEGETLLER
                     LPAVDFVLGTGRIETLPSVLRRVREEGDRPCDTGFDTDEVVYTPGAIARLVKHRASIT
                     VVEGCNKNCTFCVVPRTRGRERNRRLADVVEESRRLLDDGVVELELLGQTVNAFRDPV
                     TGEDFADLVRAVGALPGLRRLRFVTSHPKNFDAKLIDAMAGTPAVVPYLHLPVQSGSD
                     SVLKRMKRQYTRAEYVDLAGRLRERLPGLALSTDVIVGFPGETQADFEDTLSLLRELR
                     FALVYSFTYSPRPDTAAARWEHDVPPREASRRLAELMDLQQEIQRETHRALEGRVMEV
                     LVEGLDRKGVRSSGRSVCSRVVNIAQRKGEPPIPPGTFVNVLIERGYPNSLLGRLLDA
                     EARS"
     gene            13685..14170
                     /locus_tag="PLB01_06720"
     CDS             13685..14170
                     /locus_tag="PLB01_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HQQ77031.1"
                     /translation="MSIKMDVKALIVDPIANVPVVILRDADEKNFLPIWVGVFEANAI
                     ALQMEGVTTPRPMTHDLLRNVIRQIEGDVTRVVINSLEENTFYAQIHIRLANRELMVD
                     SRPSDAIALALRAHAPLFVEESVLEKSRAGDESAEAQSAERLRKWLEDADPESLGKYK
                     M"
     gene            14234..14995
                     /locus_tag="PLB01_06725"
     CDS             14234..14995
                     /locus_tag="PLB01_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQQ77032.1"
                     /translation="MIITVANQKGGVGKTTTAINIAAAFAQKGLRTLLIDLDPQGNST
                     MSFIDRKEIGKSMYEVLTDTATSIFEIIRPAALPKLTIAPARIALAKVESKLLGELDG
                     HFRLKDKLQVVKESGEYDAIIIDTPPTLGMITVNALVASSHILIPIQSSYFALEGTDD
                     LLETIEKIKGRPNPNLQILGVVITLHDRRTTLGRDIQAQIDEVFKGKVFKTTISKSIR
                     LEESPAYKESIFTFAPRSSGALEYYSLTEEILSRV"
     gene            14988..15887
                     /locus_tag="PLB01_06730"
     CDS             14988..15887
                     /locus_tag="PLB01_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQQ77033.1"
                     /translation="MSKRRGLPETSRMRHDSHFVEQLVRADELPVGRRIPIHLIEANP
                     DQPRSSMGDLAELTKSITDKGVLEPILVRPREDGRYTIISGERRFRASLDAGLTEIPC
                     IEMEVSDSELLEIALIENLQRKDLSPFEEADGYRALQERHAYTHEQIADAVGKSRVTI
                     TEALAIGRLPDAVQNECRRADIQSRSFLLELGRLKDEDLMLAMVSAMARGEDVSRDVL
                     REAKSAARRGAAPSRPGALKKAYTLDYAPEDRRYKVSLVLKGKLHKKDEILEAIRDLA
                     RRIEAGEIDLESQGRFVKAKPKK"
     gene            16155..>17473
                     /locus_tag="PLB01_06735"
     CDS             16155..>17473
                     /locus_tag="PLB01_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQQ77034.1"
                     /translation="MLRRLDRYVFTEVLAPTVVGFLAYTGFMAVRGLVQFSELLVQSE
                     QPLAEAGIVLALSLPHIVVLTLPVAFLLGLLIGIGRLSADSELTALRASGIDLAGLYR
                     PIGVLAATIAGLTLFLMLAVVPRTNHLLYTMKLRLSSFAFAQRIQPGVFSPEFGGLRV
                     YVEKASADRRELSGLIISDRSNPDEGDRLTLARKGWLELEEDQGRLWLRVEGAETHHQ
                     QDGGRNYDVTSFSKQRMVLQDNGPAGLVKEKLIREQNLQELLARAAAPSRRPEDRRLA
                     MTEVHKKFALPAACLVFGLIGLPLGIVNRRGGRAAGFAVSVGIVLLYYALIATGEARA
                     IEGRMSPAVAMWLPNALLLAFGVFALARVRRDRTVFEVAWRSPKRPEAREAAVSAAPA
                     ARRSAASRRTMRLPGLLLIDRYVARRFLATFLLVVASIAVLYVLIDYL"
BASE COUNT         2683 a         6288 c         5957 g         2545 t
ORIGIN      
        1 aggccgtcga gcgtctcgcg gccaccgcgg cgcgcggcgg gcgcgtcgtc gtcttcggcg
       61 actacgactg cgacggcatc ggcgccctcg cgatcctcac gacggcgctc cgcaagctcg
      121 gcgccgacgc gcggccgttc atcccgcacc ggctgcacga cggttacggc ctgagagcgc
      181 cggcgctccg ccgcgcgctc gacgagcacg aacccgaggg catcgtcacg gtcgactgcg
      241 gaatcaccgc cgtcgagacg gtcgccgagg ccacggggcg cggcgtgtat gtcgtcgtga
      301 cggaccacca cctcccgccg gccgcgctgc cggagggcgc ggtcctcgtg gacccgaagc
      361 tgcccggctg ccggtacccg ttcaaggagc tctgcggcgc cggcatcgcg tggaagctct
      421 cggaggcgct gctcatccac tcgggcccgc gcgtcggcat cgacgcggcc gcacggcgac
      481 gctggctcgc gtccctcgcc aagatcgcgg cgctctcgac cgtggcggac atggtgccgc
      541 tcacgggaga gaaccgcgtt ctcgtgtcgt ggggcctctc gggcctcgcc gacccgcgct
      601 cgccaggcct ctccgcgctc ctcaagcggt cgggggtccc ggcagggcgc gccccctcga
      661 cgcgcgaggt cgcgttccgg atcgcaccgc ggctgaacgc caccgggcgg atcgaccacg
      721 cggcccgggc cttcgaactc ctgacgaccg ccgacgcggc gcgcgccgag gcgctggccg
      781 acgagatcga gatcgcgaac gacgaacggc gcgccgtgca ggagaagctc gtcgagacgg
      841 tgctcgcgcg cctcgagacg acgttcgacc cggcgcggga cgcggtcgtc gtcgaggccg
      901 gcccggcctc cgagggctgg caccgcggcg tcctcgggat tgcggcgtcc cgcgtggcgc
      961 agcaggtcgg ccgccccgtg ctcctcctct cgagggacgg agagaccgtg ggcggcagcg
     1021 ggcgctcctt cggccggacg ccgctctacg agcgcgtcgc ccccgtcgcc gcgcggtaca
     1081 cgaaggagtt cggcgggcac gcggctgcgc tcggcctgac aattcccgcg gcgtcctggg
     1141 aggcgttccg cgaggacctc cgcgccgcgt tcgcggcggc gcgcgacgac gccgagtggg
     1201 cctccgacct ggaagtcgac accgaggtcg aggcgcccga agcggacgag gccctcgcgc
     1261 gggcgctcga gctcttcgag ccgcacggcc aggagaaccc caagccgctc ctgctcctgc
     1321 gcggtctgac gtgggacggg cgaggcaggc ccgtcggcga agccggactg cgcgtctcgt
     1381 tcgcgtcctc cggccgccgc ctcgacgccg tggggtggaa tctcgccgag atcccggcac
     1441 cgtcgcgcgc gggccgctgg gacgtcgcgg cgaatctcgc cgtcgactcc ttcacgggcc
     1501 ggcccggctt gacggtcgtc gacctgtccc cggaggccgc gtgagccgtt tcgcgcgcct
     1561 cgcacgcgcg gccgtcctcg tgctcggagt cgcgttcgcc ttgctcgtcg tcctctcgta
     1621 ccgcaagccg ggagcccgca cggaggacgc aaaggacccg gtggccgaga cgctcctcgc
     1681 ggaggcgaac ggcgcccgcg accgcatgcg cttccgtgac ttccggtacg acgagacgcg
     1741 cgagtccgag gggcgctacc gcgtcacggc ctccgaggcg gtgcgcttcg acgaccaggg
     1801 caaccgcctg ttccgcctga aggacgtcct gttcgagtcg cgcgagtcgc gggacggccg
     1861 caccgtctcg atccgagctc cgcgcgcgga gctcgcggag gggtcgcggg cgttccggat
     1921 cttcgacggc gtcgaggtgg cgggggagga gatgcgcgtc acgggcgcct cgttccggta
     1981 cgacccggcc cgccggctgc tcgcatcgga gggccccgtc gacgcgaccc gtggcggcct
     2041 cgtcgcgagc gcggacgcag gctccgtgga cacgcgcgac ggcgtcctcg tcctcgacgg
     2101 cgacgcgagg ctgcgggggc gaggagacga gggccggccc gtcgagctgc gcgcaccgcg
     2161 tgtcctcgtg ggacgcaacg gccggctcga ggcaagcggc ggggcggtcc tgaagacgga
     2221 ccgcttcgtc cttcgagggg agacgttcgt ccgggaggcc acgcccgtcg gcagccacgt
     2281 ccgtgcgacg acgggtgcgc gccgcctcgt gccgccggat cgcggccagc ccccggccgc
     2341 tcttgcggtc gccggcgacg tgctcgacct ggcgctcgac gcccagggcc ggcccgcggc
     2401 cttcgaggcg agcgcggccg gagccgcccg ggtggacctc gctccgaccg agacgaccgg
     2461 cgcgcgccgc gccgaatctc cccgcttcac gggccgattc gagaacggac gcctcgccga
     2521 gctgaacgtg cccgaggacc tgcgcaccgc cgagtcggcc cgcgccggag gcccgccggg
     2581 ctcgggcctc cgcacgttcg cggccggctt cgcgcgtctc acgttcgggt ccggcctcgc
     2641 gctcgaaacc ggcacgttcg agaaggacgt cgcggcggcg gacggcacgc gggcttccgt
     2701 caaggccccg cacgccacgc tgcgcggccg cgacgagacg gcggtcttct ccggcgagcc
     2761 cggcaccccg gccgactacc gcgacgaacg cgggacgatc cgcgcgagga cgctcgcgtg
     2821 gagccgccgg gacgagcgcg tggacgcggc cggcagcgtc aaggcctcgt acgcgggagg
     2881 cgccggccgc cgtgtcggtc tcctcggcag cgagagttcg gcgccgttct tcacggagtc
     2941 cgacacgctc cggctttcgg gccgcgcctc gaaggtcctg ctgacggggt ccgtccgcgc
     3001 gtggcagaac gaaaacgtcc tgcgctgcgg gacgctcgag ctggacgacg cggagaagac
     3061 cctgcgcgcc gagcagaacg tccgcgcctt cttccgccgg gagacgactc cggccaaggg
     3121 aaaggcgggg aaggcggccg cgcccgcggg ctccgagacc gtgaacgcct cgagcgacgt
     3181 cctgacccac cgggaggccg accggttcgt acggctggaa ggtcatgcga cgatcacgtc
     3241 cgggccctgg accatgaacg ccgacgtgac cgacatccgc ctgagcgaga cccgcgccat
     3301 cgagtacgcc gaggcgcgag gcgcggtcgt cgtggaagac cgcgccgagc accgccgcgg
     3361 agagggcacc aaggcggtct ggcgtccttc gacggaggcc gtgacgctcg agggctcgcc
     3421 cgcgaccgcc ctggacgggc gcggcaacaa gtcgaccggc gccgcgctca cgttccggca
     3481 gggcagatcc caggtcgacg tcgagacggg cggaaacgtt ccgaccgaga ccgtgctgag
     3541 gccggaggga tcgtgagcga cgagaagccg ctgctcgagg gccggggcct gcgcaaggcc
     3601 taccgcggcc gcacggtcgt caacgacgtc agcgtccgcg tggaggccgg cgaggtcgtc
     3661 ggccttctcg ggccgaacgg cgcgggcaag acgacgacct tctccatggt ggtcggcctc
     3721 gtgacgcccg acgcgggcgc cgtccttctc gacggcgaag acgtcacgac gctcccgatg
     3781 ttccggcggg cccgccgcgg cctcgggtac ctgccgcagg agccctcgat cttccgccgg
     3841 atgacggccg tcgagaacct cctcgccatc ctcgagactc ttcccggcgg ccgtacggag
     3901 cgcgaggaga cggcgcacgg cctcctcgat cgcttcaagc tctcgcacct cgcgcactcc
     3961 gtcgcggaca cgctctcggg cggcgagcgg cgccgcctcg agatcgcgcg cgcgctcacc
     4021 ctgtcgcccc ggttcatcct cctcgacgag ccgttcgccg gcatcgaccc gatcacggtg
     4081 ctggacctcc agcaggtgat ccgggacctc gccaccgccg ggatcgggat cctgatcacg
     4141 gaccacaacg tccgcgatac gctcgcgatc gtgaatcgcg gctacatcat ccattccggc
     4201 accattttcc ggacggggtc gcccgagacg ctgtcggccg acccggaggt ccgccgggtc
     4261 tacctggggg agagtttccg gctggactga ggcccgcggg ctctggacgg ggtggtcaga
     4321 gctagaatcg tgccaaggtc ggccccggag acggggccgc cggtttcaaa tgggactcga
     4381 acagaaactc tcgctgaggc tctcgcagcg gctggtcatg acgccgacgc tgcagcaggc
     4441 catcaagctg ctgcagatga cgcgcctgga gcttcaggac gtcgtgaacc aggagctcgt
     4501 ctcgaacccg gtcctcgacg aggaggatgc cgatcaggcg ggtgggcccg acgaggagcc
     4561 gggcgccgcc gagacccgcg agctggatct cgcgtcgtcg gccgacgcgc ccgacgcggc
     4621 cctcgaagcc gccgtcgccg acgaccggga gttcgccgcg gcggccaccg acgccgacgc
     4681 ccgcgcggag tccgccgagg cgctgcccgc gccgacgtcc gacgtcatcg ccccggagcc
     4741 ggtcgtcgat tcgtccacgg gcgccaccga cgacccgttc tccgacctcg agctcgaggc
     4801 gtacttcggc gactacatgg aaggcacggc gacggccccc cggatgaacg aggagtccga
     4861 ggagttctcg ctcgagaacc gcccggagtc gccgcccggc ctcgagcccc acctcaccga
     4921 gcagctcggc gtctcggacg cgccgcccga cgtgcgcgag gcgtgcggct tcctgatcgg
     4981 caacgtggac ccggacggct gcctgcgcgt cacgctcgaa gagaccgccg aggcggtgcc
     5041 ctgttcgatc gagacggccg agaaggcgct cgcgctgctc cagtcgttcg accccgtcgg
     5101 cgtcggcggg cgcagtctcg ccgagatcct cctcctgcag gcccgggccg cgaacgtcgc
     5161 gacgccgctt ctcgtcgagc tcgtcacgca gcgcttcgcc gaactgggct cgaagcctcc
     5221 ggccctcctc gcacgccaga tgggcgtgcc cgtggaggac atccagcagg ccctcgagtg
     5281 gattcgcagg ctcgacccga agcccggccg acggtacgac tcgacccgca cgatctacgt
     5341 ggagcccgac gtggccgtcg tgaaggtcga ggacgactac atcgtcgtgt tcaacgacga
     5401 cgggttgccg cgcctgaagg tgtccgcgct gtaccgacgg atgctcctct cccgcgacgg
     5461 gaacctggac agcgagggga agagctacct gcgcgagaag atgcgcgcgg cacagtggct
     5521 cctcaagagc ctcgatcagc ggaagcgcac gatcgtgcgc gtcgcggagt cgatcgtgaa
     5581 gaaacagcgc gacttcctcg actggggcgt cgcgcatctc aagccgctcg tcctccgcga
     5641 cgtcgccgag gacatcggga tgcacgagag cacggtctcg cgcgtcgtct cgaacaagtg
     5701 gatggcgacg ccgcgcggcc tcgtgcccat gaagttcttc ttccactcgg cgatcgcgtc
     5761 gacgggcgga gaggacgtct cgtccctcgc ggtgaagaac aagatccggg gcctcatcga
     5821 ggccgaggac ggggcccatc cgctctccga cgcccggctt gccgagctgc tggcgcacga
     5881 gggaatccag atcgcccgcc ggacggtggc gaaataccgg gaggaactcc ggatccccgc
     5941 gagctcgatc cgccgcacgg gcgaaacggc gccggcgttc gagcgtcccg agggtgcggt
     6001 cgacgccgcg gccgaggacg cggccgccga cgcggagcct tccgaacagg aggagccatg
     6061 aagaccgact acaccgcgcg tcacatccga atccactccg ggctccgcga ggtcgtcgag
     6121 acgaagctgg acaggctcga gcgcgtcctc ccgccccgcg ccgtggcgcg cgtcgtcgtg
     6181 accgcgggca agatggacgt cgaggtggaa gtttcggtcg tggggaagac ccggacgatc
     6241 acggcggccg ctcgcgccgc cgaccagcga accgccgcgc aggaggcgat ggagcgtctc
     6301 gccgggcagg tccgcaagac gaaggcgatc gtcaaggaag ccaagaagca tcgggcctcg
     6361 ggcgttcggg gtgccggcac gtgggccgag cccgagcccg cgcccgcgcc ggtgcccggc
     6421 ccgaagcgcg aggccgtgag cccgcgggcg atgttcgagg aggacgcgct gacgcacttt
     6481 gccgcctcga agcgcgagtt catggtcttc cgtgacgcgg gatccgggca tcagatccgc
     6541 tttctgtacc ggcggcgcga cgggggcttc cacctcgtcg tgcccgagta gccgtgcgca
     6601 acccgcccga gatgcgggcg cccctcgtgc ggtcgatcga cctcaggtcg ccggtcctcg
     6661 cgtccctgaa cctcaagctc gtcgcgggcc accagggtct ccagcgtccc atcgactggc
     6721 cgcgcgtcca gaagcccggg ctcgcgatcg cgggattcct cgattacgtg aaggacaacc
     6781 gcgtccagat cctgggcgcg tctgaattca actacctgaa gacgctgccg ccctcggtcg
     6841 tgcgcgagag gctcgacgcg ttcacgaacc ttcccctctc ggccgtcgtc gtcaccaagg
     6901 gaatccaccc cgggcccgtc ctcacgggcc tctgccgccg gcgccacatc cctctcttca
     6961 cgaccgagcg gctcacgagc gtcgtcatcg agggcctcac ggcgtttctc gaggaatccc
     7021 tcgcgccccg ggcgacgctg cacggcgtcc tcatggaagt cggcgggctc gggacgctcc
     7081 tcctcggcga ctccggcgtc ggcaagagcg aatgtgcgct ctcgctcgtc cagcgcggcc
     7141 accgcctcgt cgcggacgac ctcgtcgtgg tccagcgctt cccgaacgac gtcctgatcg
     7201 gctcgtcctc cggcctcatc cggcaccaca tggagctgcg cggccttggc atcgtgaacg
     7261 tgccgatgct cttcggcgtc gcggccgtcc tggagcgcca caccgtggag ttcgtgatcc
     7321 agctccagga ctggaaggag gggaccgagg tcgaccgcct cggtctcgac gaggagttcg
     7381 aggagatcct cggcgtggcc gtgccccggg ttcggatgcc ggtcgcgacg ggccgcgacg
     7441 tcgcgctcct cgtcgagatc gcggcgcgca accagctcct gaagcgtcgc ggcttcaacg
     7501 cggcccggga gatcgcccag aaggtgcacg acgagatcgt ccggaaggcg gccgcggacc
     7561 acgtcgcccg ccgggtgagg cggaagttca cggcccggga gcgcgaacgg tgagcgaagg
     7621 caagcgcctc gtcatcgcga cggggctctc ggggtccggg aagagctacg tcgcgcacgc
     7681 cctcgaggac atcgggtacg cgacggtcga caaccttccg ctctcgctgc tggaggacta
     7741 cgtcggcggg gtcgcggcgg accgcacgcc gagccggcgc aatgccgtcg tcctggacgt
     7801 ccgcaacccg gatttcgcca cgcgcttccc ggagcttctc gagacgctcc gcgcgcgcgt
     7861 tccgatcacg gtcgtctttc tcgactgcga ggagaagtgc ctcctgaacc gtttctcgga
     7921 gacgcgccgc ccgcacccgc tcggcgaggg ccggtccgtg gcggaggccg tggcctacga
     7981 gcggcggctc ctggccgagg tcaaggcgaa ggccgacctc gtgatcgaca cgaccgtcat
     8041 gaccgtccac gagctgcgcg ccgccgtcgc gcagaacttc cgagagcccg gcgatcccgg
     8101 cgtcctcgcg gtgtccctcg tgtcgttcgg gttcaagcac ggccttcccg cggcgctgga
     8161 cctgtgcttc gacgtgcggt tcctcgtgaa cccgcacttc gacgcgcgtc tccgcccgct
     8221 caccggccgg aacgccgacg tggcgcgatt catcgaggcc ggagagctga ccgagccgta
     8281 ctaccggcaa ctcgccggat tcctggcgtt ctgccttccc aactaccggc gcgagaaccg
     8341 cgcgtacctc acgatcggga tcggctgcac cggcgggcgt caccgttccg tctacgtggc
     8401 cgagcgcctc gcgcgcgacc tgaccgccat ggggtacccc gtgcgggtct cgcaccgtga
     8461 cgaggcgctc gactgacatg accgcaccga ccggccccgc cgtgggcctc atcctcctgt
     8521 cgcacggacc gctcgcgaac gccctgcgcg agaccgtcca catgctcgag cccgacgagc
     8581 cggacgacat cggcgccctt tccctcgcgt gggacgaagc gaccgaagcc gccacgcgcc
     8641 gcctcgagaa ggcgatcgcc gcgcacgacc ggggccgggg aatcgtgatc ctcacggaca
     8701 tgttcggcgg gacgccgtcc aacctcgcgc tcgcgttcct cgagccgggc aaggtcgaga
     8761 tcgtgtcggg catcaacctc ccgatggtcg tgaaggctcg cgctctcgcc cgcgagggca
     8821 agagcgcccg cgagatcgcc cacgtgctcg tggaacgcgg ccggcgcgcg atcgtcgcag
     8881 cggccgagct cgtggacggc gccccgaagg gaacgccttg aaggaagagg tcctcacgct
     8941 ccggaaccgt ctcggcctcc acgcccgcgc cgccgccaag ttcgtccacg ccgccgcgag
     9001 cttcgagtcg aagatcacga tcacgaagga cggcgacgag gtcgacggca agagcatcct
     9061 cgggctgctc ctcctggccg ccgggaaggg cgcgccgctt ctcgtgcggg ccgagggtcc
     9121 ggacgaggac cgcgcgctgg ccgcgctgcg cgacctcgtg gaccggaagt tcgacgaagc
     9181 cgagtaggcg ccccgctaca ataggaaggt gaagcgtccc ctctcgttga ccggcatcgc
     9241 ggtttccccc ggcgtggccg tgggccgtgc cgtgctgtgg gtctcgcgcg aggacacgtc
     9301 gccccgccgc accctcgagc ccgaggagat cccggacgag atcgaacgct tccgccgcgc
     9361 ggccgcggcc tcggtcgccg agatcgagga aacggcgcgc cacgtcaagg agcggctcgg
     9421 ttccgagtac gcgggcatct ttcacgccca cgcgctcttt ctccaggaca aggcgttcgt
     9481 cgggcccgtc gagcggcgca tcgtccacga cgccgcgaac gcggagtggg cggtcgcgca
     9541 gacgacggaa gacctcgtgg cgcgcttccg gagccttccc gacgagaacc tcagggaccg
     9601 cgcctccgat ctcgacgacg tctcgcgcgt cctgcgccgc cacctcggcg gcgaggaaca
     9661 gtcgcgcctc aagatgagcg atctcgcggg cgaggcgatc gtcctcgtcg cggacgagct
     9721 gacgccttcg gacgccgtcc gcatcccgcg cgaccgggtc atcgcgttcg tgacggagcg
     9781 cggcggcaag acgtcccacg cggccatcct cgcccgttcc ttcggccttc cggccgtcgt
     9841 cgccgtccac ggcctcctca cggccgtggg agacggggac cgcgtcgtcg tggacgggcg
     9901 cgacggcgtc gtctggcgcg agccctccga ggacatcgtc tccctcttcc gcgaccggcg
     9961 ggactccgag gcccggcgcg agctctccct caaggagagg gcgctctcgg gcccggctca
    10021 ctccgcggac ggaactccgg tcgagatccg cgcgaacatc gagctcacga gcgagatccc
    10081 ggacgtcgtc ggctacggcg cggacggcgt cggcctgttc cgctcggagt tcctctacct
    10141 ctcgaaggaa ggcgtcgagt tcccaaccga ggaggagcag gcggccgtgt accgcgaggt
    10201 cgtctccggc ctcgcgccgc ggccggtcgt gattcgcacg tacgacctcg gcgggaagaa
    10261 gggcgcgcgg catctcgtcg gcgccgagga gatcaacccc gtcctcggcc tcaggggcgt
    10321 gcgtctctgc ttcgcccggc ccgagatgtt catgacgcag cttcgggcgc tgctggcggc
    10381 cgcgaatgcc ggcgacctcc ggatcctcgt cccgatggtc tccagcgtcg aggagtttcg
    10441 ccgcgtccgg gagctcgtga agagggcgcg cgaggaggtc ctcgcgaagg gcatcgcggt
    10501 gcccgacgac atccctctcg gcgcaatgat cgaggttcct tcggcggcga tcacggccga
    10561 cctcctcgcc cccgagaccg acttccttgc gctcgggacg aacgacctca tccagtacac
    10621 cctcgccgtc gaccgcgcga acgaaacggt ctccgagctc ttccgcccga cgcaccccgc
    10681 ggtcctgcgc ctcatcgccc aggtcgcctc tgcggcccag gcggcccgca agccgctctc
    10741 ggtctgcggc gagatggcgg ccgaccccgc gctgctgctg ctgttcctcg gcttcgggat
    10801 ccgcgagttc tcgatgggcg cgcggtccgt ccccatcctc aaggacttcg tgcgcggcgt
    10861 gaacgtcgcc gacgccgcgc gcatcgcgaa ggccgcgctg tcgctctcga cggccgacga
    10921 ggtgtcctcg ctgctggcgc aggagctgcg cagcctcccg gcgggggagc ccttgcggcg
    10981 cctggaggtc tccgcgtgac cctcggcacg agtcgcccgc gcgtcgtccc caagccctgg
    11041 ggcgaagagc ggatcttcgc cgagaccgga cgctacgccg gcaagctcct cgtcatccgc
    11101 aagggcgaaa cgctctccta ccagtaccac gtccgaaagg aggagacgat ccacgtcctc
    11161 gacggcgtgc tcggcctcca gctcgaaaag gacggcgagg cggccgacct gcgcgtgccg
    11221 cccggcgaaa cgttccacgt cactccgggg acccgccacc ggatgtacgc gcccgagggc
    11281 gactgcctcc tcgtggaagt gtcgacaccc gagctcgacg acgtcgtccg cctcgtcgac
    11341 cgctacggac gcgcggggac gtccgcgcca tgatgcgccg ccgcgttccc ggaatcctcg
    11401 cgatcgtcct gctcgcagcc gcgggcgcgg aggctcaggc gccggcgctc tcgggcctgt
    11461 ctccgacacc cacgcccgcc gcggccacga gcggagaggc cgtgccggag gcgacgccgg
    11521 ccccgaagct caagggcgtc gcagggtctg cgaccgaggc ggggcgttcc ggccagtcgc
    11581 tcgcggacgt cgtccgcgtc tccaaggagg cgcggaagga ccagaagccg aagaagtcgc
    11641 tcgggacgat cacgaacgaa tccctgcaca agggaggctc cacgcccgcg gtgacgtcca
    11701 agggcccgtc gaagactccc gcggcgtccg cggtgcggaa ggagcccacg ccggcgccga
    11761 cgtacgacgt gccccgcgac gaccagggcc gcagcgaagc ccagtggaag gcgaccatgg
    11821 accatgcgcg atccctcgtg caggacggcg agcggcgcgt gaaggagctc gagacgaaat
    11881 ccaagcagct cgagaacgac ttctacgcgc agagcgacgg ctaccggcgc gacggcgtca
    11941 tcaagcccgc gtgggacaag tcccgcgacg agctcgcgaa ggcgcgcctc gacctggacg
    12001 ccgcgcgcaa gtcgctcgag gacctgtccg aggaagcccg gcgctcgaac gcgccgcccg
    12061 gctggctgcg ctgacgccct gctaatctcg gggccgtgag tgtgaatccg catctcaagg
    12121 gcggccgccg tgtcttcgtc gagacatggg gctgccagat gaacgtcctg gacgggcggc
    12181 gcttcgccgg cctcctcgcg cgagacgggt acgacgagac gttcgacgag tcggatgccg
    12241 acgtcttcat cctcaacacc tgctccgtgc gcgacaaggc cgagcagaag gtctacgacc
    12301 gcgtcggacg tctcgcgaag ctgaagcggg agaggccggg gctcgtggtc ggcgtgtgcg
    12361 gctgcgtggc ccagcaggag ggcgagacgc tcctcgagcg ccttcccgcg gtcgatttcg
    12421 tcctcgggac cggccgcatc gagacgctgc cgtcggtgct ccggcgcgtc cgcgaggagg
    12481 gcgaccgccc gtgcgacacg gggttcgaca cggacgaggt cgtctacacg cccggcgcga
    12541 tcgcgcgcct cgtgaaacac cgcgcgtcga tcacggtcgt cgagggctgc aacaagaact
    12601 gcacgttctg cgtcgtcccg cggacacgcg gacgggagcg caaccggcgc ctcgccgacg
    12661 tcgtcgagga gagccgcagg ctcctcgacg acggcgtcgt cgagctcgag cttctcggac
    12721 agaccgtcaa cgcgttccgt gatcccgtca ccggcgagga tttcgcggac ctcgtccgtg
    12781 cggtcggcgc gctgccgggg ctgcgccggc tgcgcttcgt gacctcccac ccgaagaact
    12841 tcgacgcgaa gctgatcgac gccatggccg ggactcccgc ggtcgtcccg tacctccatc
    12901 ttccggtgca gtcgggctcg gactccgtgc tcaagcggat gaagcgccag tacacgcgcg
    12961 ccgagtacgt cgacctcgcg gggcggcttc gcgaacgcct cccgggcctt gctctctcca
    13021 cggacgtcat cgtcgggttc ccgggggaga cacaggccga ctttgaggac acgctgtcgc
    13081 tgctccggga gctgcgcttc gcgctcgtct attcgttcac gtactcgccc cggcccgaca
    13141 cggccgccgc gcgatgggag cacgacgtcc cgccccgcga ggcctcgcgt cgtctggccg
    13201 agctcatgga cctgcagcag gagatccagc gagagaccca ccgcgccctc gagggccgcg
    13261 tgatggaggt tctcgtggaa ggtctggacc ggaagggcgt ccgaagctcg ggacgatcgg
    13321 tctgcagccg cgtcgtgaac atcgctcagc ggaaagggga gcccccgata cctccgggaa
    13381 cgttcgtgaa cgtgctgatc gagcgcggct acccgaactc tctgctcgga agactgctcg
    13441 acgccgaggc ccgctcctag gttgtgagcg tttcgctgtt cgtgatgtcc gaggacaaat
    13501 tgcttcgaac ggttaatatt cgcgcccgct gccggcccac gcgaggcggc aggtcgcgag
    13561 cccggacgcc gcgttaaccc tttctaagca aatctcttag gtgctcttcg gacggtaccg
    13621 gttgcgaata tcccttgcgc aatgagcctg cgctccctag attcagtctc aggaggcgtc
    13681 ccgagtgagc atcaagatgg acgtgaaggc gttgatcgtg gatcccatcg cgaacgtccc
    13741 ggtcgtgatc ctgcgggatg ctgacgagaa gaacttcctc ccgatctggg ttggcgtctt
    13801 cgaggcgaac gccatcgcgc tccagatgga gggcgtgacg acgccacgac cgatgacgca
    13861 cgatctcctc aggaacgtca tccgccagat cgagggcgac gtgacccggg tcgtcatcaa
    13921 cagcctggag gagaacacgt tctacgccca gatccacatc aggctcgcga accgcgagct
    13981 catggtggac agccggccgt ccgatgccat cgctctggct ctgagagctc acgcgccgct
    14041 gttcgtggag gagtccgtcc tggagaagag ccgggccgga gacgagtcgg ccgaggctca
    14101 gagcgccgag cggttgcgca agtggctcga ggatgcggat ccggaaagcc tcggcaagta
    14161 caaaatgtag ctgcggtgcc cggatctcct tgaaggccgg ccgagccctc cgtatcatgc
    14221 gcgtctcgct cgcatgatca tcacagtcgc gaaccagaaa ggcggagtgg ggaagaccac
    14281 gacggcgatc aacatcgccg ccgctttcgc gcagaaaggt cttcgtaccc tactcatcga
    14341 cctcgatccc cagggaaact ccacgatgtc cttcatcgac cggaaggaga tcgggaaatc
    14401 gatgtacgag gtcctcacgg acacggcgac gtcgatcttc gagatcatcc ggccggccgc
    14461 tcttcccaag ctcacgatcg caccggctcg tatcgctctg gcgaaagtgg agtcgaagct
    14521 cctcggcgag ctcgacggtc atttccggct caaggacaag cttcaggtgg tgaaggaatc
    14581 gggggagtac gacgcgatca tcatcgacac gccgccgacg ctcggaatga tcaccgtgaa
    14641 cgcgctcgtg gcgtcctccc acattttgat cccgatccag tcctcgtact tcgcgctcga
    14701 aggcaccgac gacctgctcg agacgatcga gaagatcaag ggccgcccga acccgaacct
    14761 ccagattctc ggcgtggtca tcacgctgca cgaccggcgc acgacgctcg gtcgcgacat
    14821 ccaggcccag atcgacgaag tcttcaaggg aaaggtcttc aagacgacga tctcgaagtc
    14881 cattcgtctc gaggaatcac cggcgtacaa ggaatccatc ttcacgttcg ctccgcgctc
    14941 gtcaggcgcg ctcgagtact acagcctgac ggaggagatc ctctcccgtg tctaagcgtc
    15001 gcggcctccc cgaaacctcc cgcatgcgcc acgattcgca cttcgtcgag cagctcgtgc
    15061 gggcggacga actgccggtc ggccggcgca ttccgatcca cctcatcgag gcaaacccgg
    15121 accagccgcg tagctcgatg ggggacctcg ccgaactcac gaagtcgatc accgacaagg
    15181 gcgttctcga gcccatcctc gtgcgcccgc gcgaggacgg gcgatacacg atcatctcgg
    15241 gtgagaggcg cttccgcgcc tcgctcgacg cgggcctgac ggagatcccg tgcatcgaga
    15301 tggaggtttc cgacagcgag ctgctcgaga tcgcgctgat cgagaacctc cagcgcaagg
    15361 acctcagccc gttcgaagag gcggacggat accgggctct ccaggaacga cacgcataca
    15421 cgcacgaaca gatcgctgac gccgtgggga agtcgcgcgt cacgatcacc gaggcgctcg
    15481 cgatcggccg ccttccggat gcggtccaga acgaatgtcg gcgcgccgac atccagtcgc
    15541 gttcgttcct cctcgagctc ggccgtctca aggacgagga cctgatgctc gcgatggtgt
    15601 ccgcgatggc gcgcggagaa gacgtgagcc gcgacgtcct tcgcgaggca aagagcgccg
    15661 cccgtcgcgg cgcagctcct tccaggcccg gcgcactcaa gaaggcctac accctcgatt
    15721 acgccccgga ggatcgccgc tacaaggtga gcctcgtcct caaaggaaag ctgcacaaaa
    15781 aggacgagat cctcgaggcg atccgcgacc tcgcgcggcg aatcgaagca ggcgagatcg
    15841 acctcgagtc ccagggccgg ttcgtcaagg cgaagccaaa gaagtagcgt ccgaagagaa
    15901 ccaaagaaga acgaagagaa gagaagatct tcttagaagg ttaagagggg ttcgccgcgg
    15961 tgttgagtaa ccgcgtcgcc gaacggccga gctgagccgc ttctcacacg acctggcctg
    16021 tcgcgctgcc taacaaattc aggccaggct ctactcgtat ttaacataat aaatattatc
    16081 ggacattgcg tattggcgct ggaatcagcg gtgatccggg agaatcttcc ccttcttccg
    16141 gctaccatcg cctcgtgctt cgtcgcctgg atcggtacgt cttcacagag gtgctggcac
    16201 cgaccgtggt cggcttcctc gcctacacag ggttcatggc ggtgcgcggc ctcgtccagt
    16261 tctcggagct cctcgtccag agcgagcagc ccctggccga ggccggaatc gtcctcgcgc
    16321 tctcgcttcc gcacatcgtg gtcctcacgc ttcccgtcgc tttcctcctc ggcctcctca
    16381 tcggaatcgg aaggctgtcg gccgactcgg agctgaccgc cctcagggcc tccggcatcg
    16441 acctcgccgg cctctaccgt ccgatcggcg ttctggccgc gaccatcgcc gggctcacgc
    16501 tcttcctgat gctcgccgtc gttccccgta cgaaccacct gctctacacg atgaagctcc
    16561 gcctctcgag ctttgcgttc gcccagcgga tccagccggg ggtcttcagt ccggagttcg
    16621 gcggcctgag ggtctatgtc gagaaggcgt cggcggaccg ccgcgagctc tccggtctca
    16681 tcatctcgga ccgctcgaac ccggacgagg gcgaccgcct cacgctggcc cgaaagggct
    16741 ggctcgagct cgaggaggac cagggccgac tctggctgcg cgtcgaaggc gccgagacgc
    16801 accaccagca ggacggcggc aggaactacg acgtgacctc gttctccaag cagaggatgg
    16861 tcctccagga caacggcccg gccggcctcg tgaaggaaaa gctcatccgc gagcagaacc
    16921 tccaggagct cctcgcgagg gctgcggctc cctcccgcag gccggaggac cgccggctgg
    16981 ccatgaccga ggtccacaag aagttcgccc tccccgccgc gtgcctcgtc ttcgggctca
    17041 tcgggctccc cctcgggatc gtcaaccggc gcggcgggcg cgccgcggga ttcgcggttt
    17101 cggtggggat cgtcctcctc tactacgcgc tcatcgcgac gggcgaggct cgcgcgatcg
    17161 agggccggat gtcccccgcc gtcgcgatgt ggctgccgaa cgccctcctc ctcgcattcg
    17221 gcgtcttcgc gctcgcccgc gtccgccgcg atcgaaccgt gttcgaggtg gcgtggcgct
    17281 ccccgaagcg gcccgaggcc cgcgaggcgg ccgtttccgc ggctccagcc gcccgccgga
    17341 gcgccgcgag ccggcgcacc atgcgcctgc ccggacttct tctcatcgac cgctacgtcg
    17401 cgcgccggtt cctcgccacg ttcctcctcg tcgtcgcgtc catcgcggtc ctctacgtcc
    17461 tgatcgacta tct
//
LOCUS       DAOMFY010000056        17451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1026_length_17451_cov_8.010980, whole genome shotgun sequence.
ACCESSION   DAOMFY010000056 DAOMFY010000000
VERSION     DAOMFY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17451
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1026_length_17451_cov_8.010980"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="PLB01_06740"
     CDS             <1..1472
                     /locus_tag="PLB01_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77035.1"
                     /translation="SDDLRRHLEGLPVEARPESTAYRLGKFVRRHRAAAAAAAVAALS
                     LAVGFGVALHEAGIARAHEAVAKKRLEDVRALANTLLFELNESLVPVPGTTKARELLV
                     RRGLEYLQRLDPKSGPEDLALQRDVAAAYQRLADLQGGGNASLGDVEGARKSLAKAVE
                     LRERTAASPAATLEDRLNLARARSSLAETLAPNSEEALRLTEAAVKVAEEALALAPSN
                     ENVRKRLAIVLDTRAMVLTQRSDFEGALSVRRREAELFDLLAARPGATDNDRRNQALV
                     SRYLASLYDRLGRLADAERENRRAVEIDEARLAAAPRNAETMVDASHSLRSLATFLRR
                     SNRSVEALPHAERAAALAGEVAAKDPADVGAQLAALRARLQVAQTHTSLGRFREARAM
                     ILDGIARLESLARAGVTAGRLPGEFGAYRSALAETWFAQMDAVRGEEKAAVRREAVAA
                     YEAARRTFAEMERLGQLGPTDRVLRDTTVRRLAELAEAR"
     gene            complement(1469..2833)
                     /locus_tag="PLB01_06745"
     CDS             complement(1469..2833)
                     /locus_tag="PLB01_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQQ77036.1"
                     /translation="MIEGRRGATQLVRALGLTDCVLYVAGSMIGSGIFLSAGNVIRKA
                     AFPSWIVVVWVIGAVHAAAAGLTYAELGARRPEAGGPYVYLSETFGPIWGFLYMWALV
                     FVVMPGTVAALSTAFAEFLGSFFPALGTAAPAFTALGLPVSQGQLVAIATAFALSAWN
                     VLGIREGGRLNDVLTVVKIGSVIALVGFGLTAAKAVRPSLAFPPMNAPLAISVGGALA
                     GVLWAYDGWINLSALGAEVKNPKRDIPGGLILGVGAVALLYLGANAVYLGAAPAATLG
                     ASPRAAETAVKVLFGDGAARWLSIAVIVSVLGSLAANIIPAPRIAWAAAKDGRLPAPF
                     ARLHPKHATPAFGLAFQAVFAAALTLSGKFDELVATVSFAATFFYALGGAALFVYRRR
                     QPDAPWKTPGYPWIPAFYVVTSLVFTAAIALDAPTDAARGLVILAAGIAVYAWQRRRE
                     PERR"
     gene            2879..4984
                     /locus_tag="PLB01_06750"
     CDS             2879..4984
                     /locus_tag="PLB01_06750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HQQ77037.1"
                     /translation="MTDGPPAPRRYVLRRTVAKSYRVDYACELNEEQKAVVFAPDGPT
                     LVVAGAGSGKTRTLVYRVSRLIEDGADPASLLLLTFTNRAAREMKRRVEALIGADLVR
                     ATAGTFHSVASRLLRPHAELLGFKPNFTILDSEDTKDLLESATSDVGVPVTERRFPKG
                     DLLRTIVSLCVNTGRKLDDVITEEHPHFLPQIDAIRTVVHRYLERKVLANGMDYDDLL
                     LNWKRLMLERPEIRRSFGLRFKHVLVDEYQDVNHLQADLVDLVVKDVEKPNVMVVGDD
                     AQSIYSFRGADFEALLGFPDRHAGTSVFPLETNYRSTPEILRLADASIAQNTRRFEKT
                     LKPVRPSGVPVAVVGASDVSQQAEFVAQRVLELRDEGTPLPEIAVLYRAHHQALELQI
                     ELTRRGIPYEIRSGLRFFEQQHVKDVLAYLRLLVNPKEETSFKRLLKLLPKVGERTAA
                     TLWTAVGDLPDPVAAFCRTDFRKAPAGAQGGLRKLAATLEALRRPSLLSAPAEAIRFV
                     VDDGGYSDVAKSKFDNHQSRLDDLEALAQFALPYDGVESFLQEVTLFGEPTGETTIAG
                     EKEDERLVLSSVHQAKGLEWRAVFVIGLVEDRFPNVRAARTAPGLEEERRLFYVAATR
                     AKDELFLVHPLAAFDRYGIVVVTEPSRFLRELADDLFERWVLEPGPALPMVPAAPPAP
                     RLAGADDPTVEDDPEDVVN"
     gene            4984..6963
                     /locus_tag="PLB01_06755"
     CDS             4984..6963
                     /locus_tag="PLB01_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQQ77038.1"
                     /translation="MSRGSRTVRFGGRILYLTEDAALVRRQLAGEDLAWDANDASHAL
                     RDQISTDEITPGWVCFHHDETLGRFAYLGVTCAEGGARVTPFGEDAVRTGGFAVSVAG
                     RRRGKGSSREQAPYAERAAGIRLLVAESFERIYRQNAINLGMLVTTDFSVLERVRLGE
                     EIPLSFFTDGEDAVTRGIIEAGGLLPYTRRRLAGSVSPPALGAPPRPMTLAEKILASH
                     AVTDAAEGASGIAAVAPGDAGFVRADWRFSHEYVTPMAANAFEAELGSAARVADPESV
                     LLFRDHLALLGEVMPEERKRRGLLALADGLAARQEEFARAQGLKLHGAGDGICHSLML
                     ERYALPGQVIVGSDSHTTHAGALGALAFGIGTSEVAGAWLSRDVRVSVPATIRVELTG
                     APPAGVAAKDVMLALLAHPAVRSGGAIGKIIEYGGEAVRAMSVDERATLTNMAAEAGA
                     FSGIVAPDETAARFLAERRGLPLEEARRACAGLASDPGAVYDSVLTLDVSALAPLVAL
                     PGDPGNGVRIADLPAPVPIDIAYGGSCTAGKREDMEMLAAVLADGLASGRRVAPGVRF
                     YVQFGSRDVREWCREKGYLDLFARAGAVTIEPGCGACIAAGPGVSTRPDEVTVSAINR
                     NFPGRSGPGSVYLASPYVVAASALAGRIAAWTPGA"
     gene            6945..7442
                     /locus_tag="PLB01_06760"
     CDS             6945..7442
                     /locus_tag="PLB01_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77039.1"
                     /translation="MDSRRLTRAALAAGLLIAGGGCRWAWNLWARGDLARDVKSLLAR
                     EGILVKDVKCRMFGTLNSGACVFPLTAAQSTTLVAALKLRVLVPTEPIREFKGGCSDT
                     SPFDTGFVRAYRSSALRPPELKLRDGGSFDYVYLYHHPTSGTACVQALYTPEGVALPA
                     NSMVR"
     gene            complement(7446..7934)
                     /locus_tag="PLB01_06765"
     CDS             complement(7446..7934)
                     /locus_tag="PLB01_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77040.1"
                     /translation="MKVNMPATFVVAAMLAALPAAPQTPGGAPPSPSPSSPSKKSSSS
                     SSSPSSDRILRATLVLDAPVDAVWKLWTTEEGVKSFFAPGCRIEPKVDEKKTRLVFTH
                     GSWGDGPEWDAAYAYFDKAWGGFVLPNLVWRVAHGPVDWKARPEVRPVAPTLAVDLAP
                     KP"
     gene            8065..9183
                     /locus_tag="PLB01_06770"
     CDS             8065..9183
                     /locus_tag="PLB01_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ77041.1"
                     /translation="MSKLLKILLGLVLLAGAATGVYAFSRGGRKADGGMKLVDAKTGS
                     IVEKAVAVGQIQPRQKFSVKSKISGIVRVCRVQVGDRVKAGDPLFEIGPDPTPLELTG
                     VDRAVESAKATFQRAQLEFDRAQELYRQGITAKSDVDQKRESFELAKVALNKAEQDRD
                     LTRKGSLAKTATTGLESIIRAPAAGTILTRLVNPGDPIVPLTSYQPGTEMATIADMSD
                     LIFKGTVDEIDVGKLHVDLPARIKVGALPTDVVTGKVSRIAPQAQQKEGATLFDVEIE
                     LDANPKITLRAGYSANADLIIREKKDILTIPERLVLWEDGGKKSFVEIPDEKAKGSKV
                     EPKKIEVKLGISDGMNVEVVSGLAKGDKVVQRPPKEIS"
     gene            9185..10420
                     /locus_tag="PLB01_06775"
     CDS             9185..10420
                     /locus_tag="PLB01_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ77042.1"
                     /translation="MVSISEVLRQLLRDVRSQKLRTFLTVFGIVWGTVAVTLLLAFGT
                     GLKKQLIKNTAGLGDRICIAWPGLTSIPYQGLGKGRRIRLVEEDVDAIRREAEGLAGV
                     SSEYQTSMKLHLDLKTWPVDVSGVSPEFGRMRNLIPDAGGRFIDPVDMAEQRRVVFLG
                     DKLAKNVFGKPEDAVGKTVMLGSSPFLVIGVLKPKIQDSSYSGRDNDKASIPGTTYRA
                     LTGEKYVNNVIYQASSAFDTEPLTARILEILGKRKKFDPKDKEALSVWDTTGQFKFFD
                     TFMLAFTGFLGIVGSLTLVVGGIGVSNIMNVVVEERTKEIGIKMALGAKQRFILGQFL
                     LETLLVTGIGGAIGFAVSLGLCAVFPRFGLTDYVGVPEVSPFVAAITIGILGAIGFVA
                     GWFPAREASRLDPVVAMKL"
     gene            10420..11673
                     /locus_tag="PLB01_06780"
     CDS             10420..11673
                     /locus_tag="PLB01_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ77043.1"
                     /translation="MRTPAVVLTLFAQSSRLSKKRAFLTIAAIAWGTVAILLLLSFGE
                     GLKRQMDRNRRSTGENLAVMWPNETSKPWKGMPPGRDVRLTLDDVSFVADRMPELPAV
                     IGEVVSWRTVLTYGRKTVNGRVLGTNVVYGEARKHYPVAGGRWLTPGDEADKRRVIFL
                     GDELAKDIFGKDDPVGKTLLVNNSPFTVIGVMQHKTQMGVYGGPDERHAVIPAATFKA
                     LFGRDRLNVLVIQSRTPEEMAPALVRLNEILGAKYGYDPTDERALSTWNIVKTSKQGQ
                     NMIIGIQIFLGLIGALTLLIGGVGVANIMYAVVKERTREIGVKMALGAKARWITWPFV
                     LEGLVYTLVGGVAGMTIATLLVTGLGLIPDGSNRVLAFLGRPTLSPVIAATTAGILGA
                     IGLAAGYFPARRAAAIDPAATLRYE"
     gene            11689..12414
                     /locus_tag="PLB01_06785"
     CDS             11689..12414
                     /locus_tag="PLB01_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ77044.1"
                     /translation="MPRDARTTVPPTDDIVAMDDIRKVYDTGKVKVEALKGVSFTIRK
                     GEFVAIVGPSGSGKSTLMNLLGCLDTPTSGSYRLAGEPVAGLSRDALADVRNRRVGFV
                     FQNFNLLPQISALENVEMPMLFGGVPPKERRARAAELLAKVGLGDRLEHRPTELSGGQ
                     MQRVAVARALAMKPDMLLADEPTGNLDSTSGGDIMGLFTELWRGGGTLVVITHDMSLA
                     RRAGRVIEVHDGRILKDSAAEAA"
     gene            complement(12424..13014)
                     /locus_tag="PLB01_06790"
     CDS             complement(12424..13014)
                     /locus_tag="PLB01_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77045.1"
                     /translation="MTKTTRRFALLSLASLFALAVSVPAAASSKKFVDSDDAKEKDEP
                     QEYLKDYDKLTKGKEADWVYFTEGVDLKSFKTVSLKTWGTTNKGSKSKRAAESGPEYL
                     EQWIKKSKKLGWEVAKEGGDLRIEGNVFNAWEPSGGARFWGGWAANPGVGIELVAKDK
                     SGKTIFEIRHKAKGSTLEDAVENGLEKIVQTFEAGK"
     gene            complement(13100..13507)
                     /locus_tag="PLB01_06795"
     CDS             complement(13100..13507)
                     /locus_tag="PLB01_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77046.1"
                     /translation="MLEDGLQVLLLADVARRDDLAERRADGPVLEEVDERRARLLAQD
                     ADRGLEVGLLRRVAALDELREVREEAGREGRAVGVAGDGDLAPARGDLDAESVFEKPQ
                     VFVVDTEERAEPGLGKGERNGVGSDVSARLRRE"
     gene            13514..14167
                     /locus_tag="PLB01_06800"
     CDS             13514..14167
                     /locus_tag="PLB01_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQQ77047.1"
                     /translation="MFAIGPAGTGKTYLAVAMAAAALLEKKIKRIVLCRPAVEAGEKL
                     GFLPGDLAEKVNPYLRPLHDALYDILGYEKVGRMLERNIIEVAPLAFMRGRTLNDAFV
                     ILDEAQNTTSEQMKMFLTRMGFGSRAVVTGDVTQIDLPQGKISGLKEADRVLNGVEGV
                     KFVRFDQRDVVRHRIVQSVVTAYEAFEKQKADAAEAAAANGNGNGHSNGNGNGHRAD"
     gene            14209..14706
                     /gene="ybeY"
                     /locus_tag="PLB01_06805"
     CDS             14209..14706
                     /gene="ybeY"
                     /locus_tag="PLB01_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQQ77048.1"
                     /translation="MKRRKSQRRAGGALGTVDVLCTVAGAPPAARVRGWTRTLLSKAG
                     AKRASLSILLCGDRRMRTLNRTWRRIDRPTDVLSFLPFAESSSSPSSSSSPFLGDLVI
                     DVPYAARQARRRGHPVAREVQILLAHGLLHLLGYDHETDDGTMFRLQKKLVLAAFGGG
                     PDGAP"
     gene            14703..16007
                     /locus_tag="PLB01_06810"
     CDS             14703..16007
                     /locus_tag="PLB01_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQQ77049.1"
                     /translation="MTGFALTAGAGLLFAALFACVFVLFSGFAQSLESLSVLRRKALL
                     EANPRRFGTLLAPEHVRISRIAVRLTAQGAVLGGLLCLGSALAALGVGEPWLLSAVVI
                     LLGWIVAESLVIRWVARRGGDALLAGFAWLIPLVDFLSAPLTPLLSRLVEVEEDTPEA
                     GLAPDAAKEEATKDAEVRALLDVAREEGLLEKHEEELVSRAVDFNDRTVGQVMTPRPD
                     IVFAEADAPLDEIADLFVRTKFTRLPLVEGSIDKPVGIVHVKDVFTVLRRPDPPATAR
                     SIAREVFFAPESQTVATLLSDFRRRRHPLAIVVDEYGVVTGLATLEDLVEELVGEIAD
                     EHEDEAPPVTALGDGSWSVAGRVRVPEIATLFDVDFPHAEYDTVAGLVSEKLGRIPKP
                     GEVAHEAGLSFTVEEADRRRIHRVRVRREAPPHRDDGGAGTE"
     gene            16004..16894
                     /gene="era"
                     /locus_tag="PLB01_06815"
     CDS             16004..16894
                     /gene="era"
                     /locus_tag="PLB01_06815"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQQ77050.1"
                     /translation="MKTLRSGTVAVVGRPNAGKSTLVNALVGTKVAIVSDKPQTTRRN
                     LLGIRNDDEGQIVFVDTPGLHKPLHRLNKSMVAEAQDALREEDVRLLVVDVTAPAGAG
                     DRFVLDLLRAAPAPRICVLNKVDLVASKETLLPRLAVLGETGIFDAIVPVSALTGEGL
                     DALLDALRRHVPEGDALFPAGISTTSDDATRIAETIREKFLERTREEIPYGLGVLVEE
                     VRREAAKNLTVVRATIVVDRDGHKGIVLGAGGRLLKEAGTAARLDLEKNFGGKFFLDL
                     VVAARPGWREDPRFLTTLTS"
     gene            16947..17366
                     /locus_tag="PLB01_06820"
     CDS             16947..17366
                     /locus_tag="PLB01_06820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQQ77051.1"
                     /translation="MTTPEKPPARPDKRRAPRLRLAKGTKAQIKSTVPVELLDLSATG
                     LLIELPSALRPGSTCDLSATLAGIPFAALVRVTRCRAGGFAADEKGARVLVYQAGGEF
                     VGLAEDQMLNLSRAIEKIGGAKLASQSSAFLKRIKPA"
BASE COUNT         2630 a         6199 c         6061 g         2561 t
ORIGIN      
        1 tctccgacga cctgcgccgg cacctcgaag gccttcccgt cgaggcgagg cccgagagca
       61 cggcctaccg gctcggcaag ttcgtccggc gccaccgggc ggcggcggcg gcggcggcgg
      121 tcgccgccct ctcgctggcg gtcgggttcg gcgtggcgct tcacgaggcg gggatcgcac
      181 gcgcgcacga ggcggtcgcg aagaaacgcc tcgaggacgt ccgcgccctc gcgaacacgc
      241 tcctcttcga gctgaacgaa tcgctcgtgc cggttcccgg caccacgaag gcccgcgagc
      301 tcctcgtccg ccgcggcctc gagtaccttc agcgtctcga tccgaagtcg gggcccgagg
      361 acctcgccct gcagcgcgac gtcgcggcgg cctaccagag gctcgcggat ctgcagggtg
      421 gcgggaacgc gagcctcggc gacgtcgagg gcgcacggaa gagccttgca aaggccgtcg
      481 agctgcggga gaggacggcc gcatcgccgg cggcgacact cgaggaccgg ctgaacctcg
      541 cccgcgcacg ttcgtccctg gcggaaacgc tcgccccgaa ctcggaggag gccctccggc
      601 tgaccgaggc cgcggtgaag gtggccgaag aggccctggc cctcgcgccc tcgaacgaga
      661 acgtccgcaa gcgcctcgcg atcgtcctcg acacgcgggc gatggtcctc acccagcgga
      721 gtgacttcga aggcgccctc tcggtccgtc ggcgcgaggc agaactcttc gacctcctcg
      781 cggccaggcc cggggcgacg gacaacgacc ggcgcaatca ggcgctcgtc tcgcggtacc
      841 tcgcgagcct ctatgaccgg cttggtcggc tcgcggacgc ggagcgcgag aaccggcggg
      901 cggtcgagat cgacgaggcg cgtcttgccg ccgccccgcg caacgcggag acgatggtgg
      961 atgcctctca cagcctccga tcgctcgcga cctttctgcg gaggtccaac cggtcggtcg
     1021 aggcgttgcc gcatgccgag agggccgccg cgctcgccgg cgaggtcgcc gccaaggatc
     1081 cggcggatgt gggcgcgcag cttgcggcgc tccgggcgcg gctgcaggtc gcccagacgc
     1141 acacgtcgct cgggcgcttc cgcgaggcgc gcgcgatgat cctcgatggg atcgcgaggc
     1201 tcgaatccct cgcccgggcc ggagtaacgg ccgggcgcct tcccggcgag ttcggggctt
     1261 accgctctgc cctcgccgaa acctggttcg cgcagatgga tgcggtccgg ggcgaggaga
     1321 aggcggccgt gcgccgcgaa gccgtcgcgg cctacgaagc ggcgcgtcgg acgttcgccg
     1381 agatggaacg gctcgggcaa ctcggcccga cggatcgggt gctgcgggac acgacggtca
     1441 gaaggctcgc cgagctcgcc gaggctcgct agcggcgttc cggctcgcgc cgccgctgcc
     1501 acgcgtacac ggcgatgccg gccgcgagga tgacgagacc gcgcgcggcg tccgtcggcg
     1561 cgtcgagtgc aatcgcggcg gtgaagacga gcgacgtgac gacgtagaaa gcagggatcc
     1621 acgggtagcc gggcgtcttc cagggcgcgt ccggctgccg gcgccggtac acgaagagag
     1681 ccgcgccgcc gagcgcgtag aagaacgtcg cggcgaacga gacggtcgcg acgagctcgt
     1741 cgaactttcc cgacagcgtc agcgcggcgg cgaagacggc ctgaaaggcg aggccgaacg
     1801 cgggcgtcgc gtgcttcggg tgcaggcgcg cgaacggggc ggggagccgg ccgtccttcg
     1861 ccgcggccca cgcgatgcgc ggcgccggga tgatgttcgc ggcgagggag ccgaggaccg
     1921 agacgatcac cgcgatcgag agccagcgcg cggcgccgtc gccgaagagg accttcacgg
     1981 cggtctcggc cgcacgcggc gaggcgccga gtgtggccgc gggcgccgcg ccgaggtaga
     2041 ccgcgtttgc gccgaggtag aggagggcga ccgcgccgac gccgaggatc aggccgcccg
     2101 ggatgtcccg cttcgggttc ttcacctcgg cgccgagcgc cgacaggttg atccacccgt
     2161 cgtacgccca gaggacgccc gcgagagcgc cgcccacgga gatcgcgagg ggagcgttca
     2221 tcggcgggaa cgcgagggac ggccggacgg ccttcgcggc cgtcagcccg aagccgacga
     2281 gcgcgatcac cgagccgatc ttcacgaccg tgaggacgtc gttcagccgg ccgccttccc
     2341 tgatcccgag gacgttccag gccgaaaggg cgaacgccgt cgcgatcgcg acgagctgac
     2401 cctgcgagac cgggaggccg agcgccgtga acgcgggcgc ggcggtcccg agcgccggga
     2461 agaacgagcc gaggaactcg gcgaacgccg tcgagagggc ggcgaccgtc cccggcatca
     2521 cgacgaagac gagcgcccac atgtagagga agccccagat cggcccgaac gtctcggaga
     2581 ggtacacgta cgggccgccc gcctccggac gccgcgcgcc gagttcggcg tacgtgaggc
     2641 cggccgcggc ggcgtggacg gcgccgatca cccagacgac gacgatccac gaggggaaag
     2701 cggccttgcg gatgacgttg ccggccgaga ggaagatccc gctcccgatc atcgagccgg
     2761 cgacgtagag gacgcagtcc gtgagcccga gggccctcac gagctgcgtc gcgccgcggc
     2821 gtccttcgat cacgcgcggg gaagcctagc aggcgtcccg gaggggtaat ctccgggcgt
     2881 gacggacggc ccgcccgccc cccgcaggta cgtcctgcgc cgcacggtcg cgaagagcta
     2941 ccgcgtggac tacgcgtgcg agctgaacga ggagcagaag gccgtcgtct tcgcacccga
     3001 cgggcccaca ctcgtcgtcg cgggcgccgg ctcgggcaag acgcggacgc tcgtctaccg
     3061 cgtctcgcgc ctcatcgagg acggcgccga cccggcctcg ctcctcctcc tcacgtttac
     3121 gaaccgcgcg gcgcgcgaga tgaagcggcg cgtcgaggcc ctcatcggcg ccgacctcgt
     3181 gcgcgcgacg gccgggacgt tccattcggt cgcctcgcgg ctcctccgtc cgcacgccga
     3241 gctcctcggc ttcaagccga acttcacgat cctcgactcg gaggacacga aagacctcct
     3301 cgagtccgcg acgtccgacg tcggcgtgcc cgtcacggag cggcgcttcc cgaagggcga
     3361 cctcctgcgc acgatcgtct ccctctgcgt caacacgggg cggaagctcg acgacgtcat
     3421 cacggaggag catccgcact tcctcccgca gatcgacgcg atccgcacgg tcgtccaccg
     3481 ctacctcgag cggaaggtcc tcgcgaacgg catggactac gacgacctcc tcctgaactg
     3541 gaagcggctc atgctcgagc gcccggagat ccggcgctcc ttcgggctga ggttcaagca
     3601 cgtcctcgtg gacgaatacc aggacgtgaa ccacctgcag gccgacctcg tggacctcgt
     3661 cgtgaaggac gtcgagaagc cgaacgtcat ggtcgtgggc gacgacgcgc agtccatcta
     3721 ctcgttccgc ggcgcggact tcgaggccct gctcggcttc cccgaccgcc acgcgggcac
     3781 gtcggtcttt ccgctggaga cgaactaccg ctcgacgccc gagatcctgc gcctcgcgga
     3841 cgcctcgatc gcgcagaaca cgcggcggtt cgagaagacg ctcaagcccg tgcggccctc
     3901 cggcgtcccc gtggccgtcg tcggcgcgtc ggacgtctcg cagcaggccg agttcgtcgc
     3961 gcagcgcgtc ctcgagctgc gcgacgaggg gacgccgctc cccgagatcg cggtcctcta
     4021 tcgcgcgcac caccaggcgc tcgagctcca gatcgagctc acgcgccggg gcatcccgta
     4081 cgagatccgc tcgggcctgc gcttcttcga gcagcagcac gtcaaggacg tcctcgcgta
     4141 cctgcgcctc ctcgtgaacc cgaaggagga gacgtcgttc aagcgcctcc tcaagcttct
     4201 ccccaaggtc ggcgaacgca ccgccgcgac gctctggacg gccgtcggcg acctgccgga
     4261 tcccgtcgcg gctttctgcc ggacggactt ccggaaggcg cccgcgggcg cccagggcgg
     4321 cctgaggaag ctcgcggcga ccctggaggc gctgcgccgc ccgtccctcc tctccgctcc
     4381 ggccgaggca atccgcttcg tcgtggacga cggcgggtac tccgacgtcg cgaagtccaa
     4441 gttcgacaac caccagtccc gcctcgacga cctcgaggca ctcgcgcagt tcgccctccc
     4501 gtacgacggc gtcgagagct tccttcagga ggtcacgctc ttcggagagc ccacgggcga
     4561 gacgacgatc gcaggggaga aggaggacga gcgcctcgtc ctctcgtccg tccaccaggc
     4621 caagggtctc gagtggcgcg ccgtgttcgt gatcgggctc gtcgaggacc gtttcccgaa
     4681 cgtgcgggcc gcccggacgg ccccgggcct cgaggaggag cgccgcctct tctacgtcgc
     4741 ggcgacgcgc gccaaggacg agctcttcct ggtccacccg ctcgccgcgt tcgaccggta
     4801 cggcatcgtc gtcgtgacgg agccgagccg gttcctgcgc gagctcgcgg acgacctctt
     4861 cgaacgctgg gtcctcgagc cgggccccgc gctgccgatg gtgccggccg cgcctcccgc
     4921 cccgcgcctc gccggggcgg acgacccgac ggtggaggac gaccccgagg acgtggtgaa
     4981 ctgatgtccc gcgggagccg gacggtgcgc ttcggcggcc gcatcctcta cctgaccgag
     5041 gacgcggccc tcgtgcgccg gcagctcgcg ggtgaggacc tcgcctggga cgcgaacgac
     5101 gcctcccacg ccctgcggga ccagatctcg acggacgaga tcactccggg atgggtctgc
     5161 ttccaccacg acgagaccct cggccgcttc gcgtacctcg gggtcacctg cgccgagggc
     5221 ggcgcgcgcg tgacgccgtt cggcgaagac gccgtgcgca cgggcggctt tgccgtctcg
     5281 gttgccgggc ggcgccgcgg caagggctcc tcccgcgagc aggcgcccta cgccgagcgc
     5341 gcggccggca tccgcctgct cgtcgccgag agcttcgagc gcatttaccg gcagaacgcg
     5401 atcaacctcg ggatgctcgt cacgacggac ttctcggtcc tcgagagggt gcgcctcggc
     5461 gaggagatcc cgctctcgtt cttcacggac ggcgaggacg ccgtcactcg cgggatcatc
     5521 gaggccggcg gcctcctccc gtacacgcgc cgccgcctcg ccgggtcggt ctcgcccccc
     5581 gctctcggcg ccccgccgcg cccgatgaca ctggccgaga agatcctcgc gtcccacgcg
     5641 gtcacggacg cggccgaggg cgcttcgggc atcgcggcgg tcgcgccggg cgacgcgggc
     5701 ttcgtccggg cggactggcg cttctcgcac gagtacgtga cgcccatggc cgcgaacgcc
     5761 ttcgaggccg agctcgggtc cgcggcccgc gtcgcggatc cggagagcgt ccttctcttc
     5821 cgcgaccatc tcgccctcct cggagaggtc atgccggagg aacggaagcg gcgcggcctc
     5881 cttgcgctcg cggacggcct cgcggcgcgc caggaggagt tcgcacgcgc gcagggcctg
     5941 aagcttcacg gcgcgggcga cgggatctgc cactccctga tgctcgaacg ctacgccctg
     6001 cccgggcagg tcatcgtcgg ctcggactcg cacacgacgc acgcgggcgc gctcggcgct
     6061 ctcgcgttcg ggatcgggac ctcggaggtc gccggcgctt ggctctcgcg ggacgtgcgc
     6121 gtgagcgtcc cggcgacgat ccgcgtcgag ctgacgggtg cgccgccggc cggcgtcgcg
     6181 gccaaggacg tcatgctcgc tcttctcgcg cacccggccg tccggagcgg cggcgcgatc
     6241 gggaagatca tcgagtacgg cggcgaggcg gtgcgcgcga tgtccgtcga cgagcgggcg
     6301 acgctcacga acatggcggc cgaggccggc gccttctcgg ggatcgtcgc gcccgacgag
     6361 acggcggccc gctttctcgc cgagcggcgg ggcctcccgc tcgaggaggc gcgccgcgcc
     6421 tgcgcagggc ttgcctccga tcccggcgcc gtctacgact cggtcctcac gctcgacgtc
     6481 tcggcgctcg ctccgctcgt cgcgctgccg ggagaccccg ggaacggcgt ccggatcgcc
     6541 gacctgcccg cgcccgtccc catcgacatc gcctacggag ggtcgtgcac ggccggcaag
     6601 cgggaggaca tggagatgct cgcagccgtg ctcgccgacg gcctcgccag cgggcgccgc
     6661 gtggcgcccg gcgtgcgctt ctacgtccag ttcgggagcc gtgacgtgcg cgaatggtgc
     6721 cgggagaagg gctacctcga cctcttcgcg cgtgcgggag ccgtcacgat cgagccgggc
     6781 tgcggcgcgt gcatcgcggc gggccctggt gtttcgacgc ggccggacga ggtgaccgtc
     6841 agcgcgatca accgcaactt tccgggccgg agcgggccgg ggtccgtgta ccttgcgagc
     6901 ccgtacgtcg tcgcggcgag cgctctcgcg ggtaggattg cggcgtggac tcccggcgcc
     6961 tgacccgagc cgctctcgcc gcgggcctgc tcatcgccgg aggcggctgt cgctgggcct
     7021 ggaacctctg ggcgcgcggc gacctggccc gcgacgtgaa gtccctcctc gcgcgcgaag
     7081 gcatcctcgt gaaagacgtg aagtgccgga tgttcgggac gctcaactcg ggcgcgtgcg
     7141 ttttcccgct gacggcggct cagtcgacga cgctcgtggc cgctctgaag ctccgtgttc
     7201 tcgtcccgac ggagccgatc cgggagttca agggcgggtg ctcggacacg tcccccttcg
     7261 acacggggtt cgtccgggct taccggtcct cggcgctccg accgccggag ctcaagctcc
     7321 gcgacggcgg gtcgttcgac tacgtctacc tgtatcacca tccgacgtcg gggacggcct
     7381 gcgtccaggc gctctacacg cccgagggag tggctcttcc cgcgaactcg atggtccgct
     7441 gagcgtcagg gcttcggcgc gaggtcgacg gcgagcgtcg gcgcgacggg cctgacctcg
     7501 gggcgcgcct tccagtcgac ggggccgtgc gcgacgcgcc agacgaggtt cgggagcacg
     7561 aacccgcccc aggccttgtc gaaataggcg tacgcggcgt cccactcggg cccgtcgccc
     7621 cagctgccgt gcgtgaagac gagacgggtc ttcttctcgt ccaccttcgg ctcgatccgg
     7681 cagcccggcg cgaagaagct cttcactccc tcttccgtcg tccaaagctt ccagacggcg
     7741 tcgacgggcg cgtcgaggac gagcgtggcc cggaggatgc ggtcggagga gggagacgaa
     7801 gacgaagagg aagatttctt agaaggtgaa gagggggagg gcgacggcgg cgcgccgccg
     7861 ggagtctgcg gtgcggcggg taacgccgcc agcatcgcgg cgacgacgaa tgtcgcgggc
     7921 atgttcactt tcatggaaat ccccctttct cgctccacgc cccggccggc tcgccggaga
     7981 aggcccgaaa actgcggcgg aaccttcggc cgcccccggc gtattccact ttagacgcag
     8041 ggctccgccc gggaggcatg ggaaatgtcc aagttgttga aaatccttct cggactggtc
     8101 cttctggccg gggcggccac gggtgtctac gcgttcagcc gtggggggcg gaaggccgac
     8161 ggcggaatga agctcgtgga cgccaagacg gggtcgatcg tcgaaaaggc ggtcgcggtc
     8221 gggcagatcc agccccgcca gaagttctcg gtcaagtcga agatctcggg catcgtgcgg
     8281 gtctgccgcg tccaggtcgg cgaccgggtg aaagccggcg acccgctctt cgagatcggc
     8341 ccggacccga cgccgctcga gctgaccggc gtcgaccgcg cggtcgaatc cgcgaaggcg
     8401 acgttccagc gcgcccagct cgagttcgac cgggcccagg agctgtaccg gcaggggatc
     8461 acggcgaagt cggacgtgga ccagaagcgg gagagtttcg agctcgcgaa ggtggcgctc
     8521 aacaaggccg agcaggaccg cgacctgacg cgcaagggca gcctcgcgaa gacggccacg
     8581 acggggctcg agtcgatcat ccgtgcgcct gcggccggga cgatcctgac ccgcctcgtg
     8641 aacccgggcg accccatcgt cccgctcacc tcgtatcagc cgggcaccga gatggcgacg
     8701 atcgcggaca tgagcgacct catcttcaag ggcacggtgg acgagatcga cgtcggcaag
     8761 ctccacgtcg atctgccggc ccgcatcaag gtgggcgccc tcccgacgga cgtcgtgacg
     8821 ggcaaggtct cccgcatcgc ccctcaggcc cagcagaagg agggcgcgac gctcttcgac
     8881 gtcgagatcg agctcgacgc gaatccgaag atcacccttc gcgccggata ctcggcgaac
     8941 gccgacctca tcatccggga gaagaaggac atcctcacga ttcccgagcg cctcgtcctc
     9001 tgggaggacg gcgggaagaa gagcttcgtc gagatcccgg acgagaaggc caagggcagc
     9061 aaggtcgagc cgaagaagat cgaggtcaag ctcggcatct cggacggaat gaacgtcgag
     9121 gtcgtgagcg gcctcgccaa gggcgacaag gtggttcaga ggccgcccaa ggagatctcc
     9181 tgaagtggtc tcgatctccg aggtcctccg ccagttgttg agggacgtgc gcagccagaa
     9241 gctgcgcacg ttcctgacgg tgttcggcat cgtctggggg accgtcgccg tgacgctgct
     9301 cctcgcgttc ggcacgggcc tcaagaagca gctgatcaag aacacggcgg gcctcggcga
     9361 ccggatctgc atcgcgtggc cgggcctcac gtcgatcccg taccagggcc tcggcaaggg
     9421 ccgcaggatc cgcctcgtcg aggaggacgt cgacgcgatc cgccgcgagg ccgagggcct
     9481 cgccggcgtc tcgagcgagt accagacgtc catgaagctc cacctcgacc tcaagacgtg
     9541 gcccgtggac gtctcgggtg tctcgccgga gttcggccgg atgcgcaacc tgatcccgga
     9601 cgccggcggc cggttcatcg accccgtgga catggccgag cagcggcgcg tcgtcttcct
     9661 cggcgacaag ctcgcgaaga acgtcttcgg caagccggag gacgcggtcg ggaagaccgt
     9721 catgctcggg agctcgccct tcctcgtgat cggcgtcctc aagccgaaga tccaggactc
     9781 gagctacagc gggcgcgaca acgacaaggc ctcgatcccg ggcacgacgt accgcgcgct
     9841 cacgggcgag aaatacgtca acaacgtgat ctaccaggcg agctcggcgt tcgacaccga
     9901 gccgctcacg gcccgcatcc tcgagatcct cggcaagcgc aagaagttcg acccgaagga
     9961 caaggaagcc ctctccgtgt gggacacgac ggggcagttc aagttcttcg acacgttcat
    10021 gctcgccttc acggggttcc tcggcatcgt cggttcgctg acgctcgtcg tcggcggcat
    10081 cggcgtctcc aacatcatga acgtcgtcgt cgaggagcgg acgaaggaga tcgggatcaa
    10141 gatggccctc ggggcgaagc agcgcttcat cctcgggcag ttcctcctcg agacgctcct
    10201 cgtgacgggc atcggcggcg cgatcgggtt cgccgtctcg ctcggcctct gcgccgtgtt
    10261 tccgcggttc gggctcacgg actacgtcgg ggtccccgag gtgtctccgt tcgtcgcggc
    10321 gatcacgatc ggcatcctcg gggcgatcgg attcgtcgcc gggtggttcc cggcgcggga
    10381 ggcgtcgcgg ctggatccgg tcgtggcgat gaagctctga tgcgaactcc cgccgtcgtc
    10441 ctgaccctct tcgcgcagtc ctcgcggctc tcgaagaagc gcgcgttcct cacaatcgcg
    10501 gcgatcgcgt gggggacggt cgcgatcctc ctcctcctct ccttcggcga agggctcaag
    10561 cgccagatgg accgcaaccg gcgctccacc ggcgagaacc tcgccgtcat gtggccgaac
    10621 gagacgtcga agccctggaa aggcatgccc cccgggcggg acgtccggct cacgctcgac
    10681 gacgtctcgt tcgtggcgga ccggatgccg gagctcccgg ccgtgatcgg cgaagtcgtg
    10741 tcgtggcgca ccgtcctcac ctacgggcgc aagacggtga acgggcgcgt cctcgggacg
    10801 aacgtcgtct acggggaggc gcgcaagcat tacccggtcg cgggcggacg gtggctcacg
    10861 cccggggacg aggccgacaa gcgccgcgtc atcttcctcg gggacgagct ggccaaggac
    10921 atcttcggca aggacgaccc cgtggggaag accctcctcg tgaacaactc tccgttcacg
    10981 gtcatcggcg tcatgcagca caagacgcag atgggcgtct acggcggacc cgacgagagg
    11041 cacgccgtga tcccggcggc gacgttcaag gccctcttcg gccgcgaccg gctgaacgtc
    11101 ctcgtgatcc agtcgagaac gccggaggaa atggcgccgg cgctcgtccg cctgaacgag
    11161 atcctcgggg cgaagtacgg ctacgacccg accgacgagc gtgcgctctc gacgtggaac
    11221 atcgtgaaga cgtcgaagca aggccagaac atgatcatcg ggatccagat ctttctcggc
    11281 ctgatcggcg ccctgaccct tctcatcggc ggcgtcggcg tcgcgaacat catgtacgcc
    11341 gtcgtgaagg agcggacgcg ggagatcggc gtgaagatgg ccctcggcgc gaaggcgcgc
    11401 tggatcacgt ggcccttcgt cctcgagggg ctggtctaca cgctcgtggg cggagtcgcg
    11461 gggatgacga tcgcgactct cctcgtgacc ggcctcggcc tcatcccgga cggctcgaac
    11521 cgcgttctcg cgttcctcgg ccgtccgacc ctctctcccg tcatcgccgc gacgaccgcc
    11581 gggatcctcg gcgcgatcgg cctcgcggcc ggctacttcc cggcccgccg cgccgcggcc
    11641 atcgaccccg cggcgaccct gcgctacgag taagacgaag gagaccgcat gccccgcgac
    11701 gccaggacca ccgtcccgcc caccgacgac atcgtcgcca tggacgacat ccggaaggtg
    11761 tacgacacgg gcaaggtgaa ggtcgaggcc ctcaagggcg tgagcttcac gatccggaag
    11821 ggcgagttcg tcgccatcgt cgggccgtcg ggctcgggca agtccaccct catgaacctt
    11881 ctcgggtgtc tcgacacgcc cacgagcggg tcctaccgcc tcgcggggga gccggtcgcg
    11941 gggctctcgc gcgacgcgct cgccgacgtg aggaaccgcc gcgtcgggtt cgtcttccag
    12001 aacttcaacc tcctgcccca gatctcggcg ctcgagaacg tcgagatgcc gatgctcttc
    12061 ggcggtgtgc cgccgaagga gcgccgcgcc cgcgcggccg agctgctcgc gaaggtggga
    12121 ctcggcgacc gcctcgagca caggcccacg gagctgtccg gcggccagat gcagcgcgtg
    12181 gcggtcgccc gggccctcgc gatgaagccc gacatgctcc tcgcggacga gcccaccggg
    12241 aacctcgact cgacgtccgg cggggacatc atgggcctct tcacggagct ctggaggggc
    12301 ggcggcaccc tcgtcgtgat cacgcacgac atgagcctcg cccggcgcgc cggccgggtc
    12361 atcgaggttc acgacgggcg gatcctgaag gactccgcgg ccgaggcggc ctgagccgtc
    12421 cgcttacttg ccggcttcga acgtctggac gatcttttcg aggccgttct cgacggcatc
    12481 ctccagcgtc gagcccttcg ccttgtggcg gatctcgaag atcgtcttcc ccgatttgtc
    12541 cttcgcgacg agctcgatcc ccacgcccgg attcgccgcc cagccgcccc agaagcgggc
    12601 gccgccgctc ggctcccagg cgttgaagac gtttccttcg atccgcaggt cgccgccctc
    12661 cttcgcgacc tcccagccga gcttcttgga cttcttgatc cactgctcca ggtactcggg
    12721 acccgactcc gccgcgcgct tgctcttcga gcccttgttc gtggtgcccc atgtcttcag
    12781 cgagacggtc ttgaacgact tgaggtcgac gccctcggtg aagtagaccc agtcggcctc
    12841 cttccccttc gtcagcttgt cgtagtcctt caggtactcc tggggctcgt ccttttcctt
    12901 cgcgtcgtcc gaatccacga acttcttgga gctggccgcg gcgggaacgc tgacggcgag
    12961 cgcgaagagc gaggcgagcg acagcagggc gaagcggcgg gtcgtcttgg tcatggtcgg
    13021 actcctcctc gggttggttt ttgtctcgct ggacagggga ttgtaagaag agagcaagag
    13081 aaggggaaga ttcctgttat cattcccgtc ggaggcgcgc actcacatcc gacccgacgc
    13141 cattcctctc acccttcccg aggccgggct cggcgcgctc ttcggtgtcc acgacgaaaa
    13201 cctgaggctt ctcgaagacg ctttcggcgt cgagatcgcc gcgcgcgggc gcgaggtctc
    13261 cgtctccggc gaccccgacg gcacggccct cgcggcccgc ctcctctcgg acttcgcgaa
    13321 gctcatcgag cgcggctaca cgccgaagaa gcccgacctc gaggccgcga tccgcatcct
    13381 gcgcgaggag ccgggcacgt cgctcgtcga cttcttcgag aaccggtccg tcggcccggc
    13441 gctccgccag gtcgtctcgc cgcgcaacgt ccgccagcag gagtacctgc aggccgtcct
    13501 cgagcacgac atcgtgttcg cgatcgggcc ggccggcacg ggcaagacgt acctcgccgt
    13561 cgcgatggcg gcggccgcgc tcctcgagaa gaagatcaag cgcatcgtcc tctgccgccc
    13621 cgccgtcgag gcgggcgaga agctcggctt cctcccgggc gacctcgccg agaaggtgaa
    13681 cccgtacctg cgcccgctgc acgatgcgct ctacgacatc ctcggatacg agaaggtcgg
    13741 gcgcatgctc gagcggaaca tcatcgaggt ggcgcctctc gcgttcatgc gcggccgcac
    13801 gctgaacgac gcgttcgtga tcctcgacga ggcccagaac acgacgtccg agcagatgaa
    13861 gatgttcctg acgcgcatgg gattcggcag ccgggccgtc gtgacgggcg acgtcaccca
    13921 gatcgacctc ccgcagggaa agatctcggg cctgaaggaa gccgatcgcg tcctgaacgg
    13981 cgtcgagggc gtcaagttcg tccgcttcga ccagcgcgac gtcgtccgcc accgcatcgt
    14041 ccagtccgtc gtgaccgcct acgaggcctt cgagaagcag aaggccgacg ccgccgaagc
    14101 cgccgcggcg aacgggaacg gcaacggcca ctcgaacggc aacgggaacg gtcaccgggc
    14161 cgactgacgc ttcggttttc gccgcggcac attaagattt cttagaaggt gaagagaaga
    14221 aagtcgcaaa ggcgggcggg cggagccctc ggcaccgtcg atgtcctgtg cacggtggcg
    14281 ggcgcgccgc cggcggcgcg ggtgcgcggc tggacgcgca cactcctttc gaaggcgggc
    14341 gcgaagcgcg cctccctctc gatcctcctc tgcggcgacc gccgcatgcg caccctgaac
    14401 aggacgtggc gccgcatcga ccgcccgacc gacgtcttgt cttttcttcc attcgcagaa
    14461 tcttcatctt ctccttcttc ctcttcttct ccattcctcg gcgacctcgt gatcgacgtt
    14521 ccgtacgcgg cccggcaggc gcgccggcgg gggcatcccg tcgcgcgaga ggtgcagatc
    14581 ctcctcgccc acggcctcct ccaccttctc ggatacgatc acgagacgga cgacggcacg
    14641 atgttccgcc tgcagaaaaa actcgtcctc gcggccttcg gcggcggccc ggacggcgcg
    14701 ccgtgacggg cttcgcgctc accgcaggcg ccggcctgct cttcgcggcg cttttcgcgt
    14761 gcgtcttcgt cctcttctcg ggcttcgccc agtcgctcga gagcctctcc gtcctccgcc
    14821 ggaaggcgct cctcgaggcg aatccgcggc gcttcgggac gctgctcgcc cccgagcacg
    14881 tgcgcatctc gcgaatcgcc gtgcgcctca cggcccaggg cgccgtcctc ggcggcctcc
    14941 tctgcctcgg ctcggcgctg gcggcgctcg gcgtcggcga gccgtggctc ctgtccgccg
    15001 tcgtgatcct cctcggctgg atcgtcgcgg agagcctcgt gatccgctgg gtcgcccggc
    15061 ggggcggcga cgccctcctc gcgggcttcg cgtggctgat cccgctcgtg gactttctct
    15121 cggcgccgct cacgccgctc ctctcgcgcc tcgtcgaggt cgaggaggac actccggagg
    15181 cgggcctcgc gccggatgcg gcgaaggagg aggcgacgaa ggacgccgag gtgcgcgcgc
    15241 tcctcgacgt cgcccgcgag gagggccttc tcgagaagca cgaggaggag ctcgtctccc
    15301 gcgccgtgga cttcaacgac cggaccgtgg ggcaggtgat gacgccgcgc ccggacatcg
    15361 tgtttgccga ggccgacgcg cccctcgacg agatcgccga cctcttcgtg aggacgaagt
    15421 tcacgcgcct tccgctcgtg gaaggctcga tcgacaagcc cgtcgggatc gtccacgtca
    15481 aggacgtctt cacggtgctg cgccgcccgg acccgcccgc caccgcgcgt tcgatcgcgc
    15541 gcgaggtctt cttcgcgccc gagtcccaga cggtcgcgac gcttctctcc gatttccgca
    15601 ggcgccggca cccgcttgcg atcgtggtgg acgagtacgg ggtcgtgacg ggcctcgcga
    15661 cgctggagga cctcgtcgag gagctcgtcg gcgagatcgc ggacgagcac gaggacgagg
    15721 cgccgcccgt gacggccctc ggcgacggct cgtggtccgt ggcgggccgc gtgcgcgtcc
    15781 ccgagatcgc gacgctcttc gacgtggact ttccgcacgc cgagtacgac acggtcgccg
    15841 ggctcgtctc cgagaaactc ggccgtatcc cgaagcccgg cgaggtggcg cacgaggccg
    15901 gcctctcctt cacggtggag gaggcggacc gccgccggat tcatcgcgtg cgcgtgcgcc
    15961 gcgaggcccc gccgcaccgc gacgacggcg gggcggggac cgagtgaaga ccctccgctc
    16021 cgggacggtc gccgtcgtcg ggcgcccgaa cgccggcaag tcgacgctcg tgaacgcgct
    16081 cgtcgggacg aaggtcgcga tcgtctccga caagcctcag acgacgcgcc gcaacctcct
    16141 cggaatccgg aacgacgacg aggggcagat cgtgttcgtc gacacgccgg ggctccacaa
    16201 gcccctccac cggctgaaca agtccatggt cgccgaggcg caggacgccc tgcgtgagga
    16261 ggacgtgcgc ctcctcgtcg tcgacgtcac cgcgccggcg ggcgcggggg accggttcgt
    16321 cctcgacctc ctgagagccg cgccggcgcc gcggatctgc gtcctgaaca aggtcgacct
    16381 cgtcgcgtcg aaggagacgc tcctgccgcg cctcgccgtt ctcggcgaga cggggatctt
    16441 cgacgcgatc gttccggtct cggcgctcac cggtgagggc ctcgacgccc tcctggacgc
    16501 gctgaggcgc cacgtccccg agggggacgc gctcttcccg gcgggcatct cgacgacgtc
    16561 cgacgacgcg acgcggatcg cggagacgat ccgtgagaag ttcctcgaac ggacgcgcga
    16621 ggagatcccg tacgggctcg gcgtcctcgt ggaggaagtc cgccgtgagg cggcgaagaa
    16681 cctgacggtc gtgcgcgcga cgatcgtggt cgaccgcgac ggccacaagg gaatcgtcct
    16741 cggcgcaggc gggcgcctcc tgaaggaggc gggcacggcg gcccgcctcg acctcgagaa
    16801 gaatttcggc ggcaagttct tcctcgacct cgtcgtcgcg gcgcggcccg gctggcgcga
    16861 ggacccgcgg ttcctcacga cgctcacaag ctgagcgttg acgcggaacg gccgttgcca
    16921 taagactccg ttcggaggac tgcgccctga cgaccccgga aaagccgccc gcaaggccag
    16981 acaagcgccg cgcaccgcgg cttcggctcg cgaagggaac gaaggcccag atcaagtcga
    17041 cggtgcccgt cgagcttctg gacctttcgg cgaccggcct cctgatcgag ctgccgtcgg
    17101 ccttgcgccc gggctccacg tgcgacctct ccgccacgct cgcgggcatc ccgttcgcgg
    17161 ctctcgtgcg ggtcacgcgc tgccgcgcgg gcggattcgc cgcggacgag aagggcgcgc
    17221 gggtcctcgt ctaccaggcg gggggcgagt tcgtgggcct ggccgaggac cagatgctga
    17281 acctctcgcg cgcgatcgag aagatcggcg gcgccaagct cgcgagtcag tcctcggcgt
    17341 tcctgaagcg aatcaagccg gcctagggag cctgctctcc ggccgagagg tcgcgcagcg
    17401 cggcccggag ggcctcctcg agccggcgcg cgcggtcgga ggccgccgcg g
//
LOCUS       DAOMFY010000057        17047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1050_length_17047_cov_6.934793, whole genome shotgun sequence.
ACCESSION   DAOMFY010000057 DAOMFY010000000
VERSION     DAOMFY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17047
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_17047_cov_6.934793"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..753
                     /locus_tag="PLB01_06825"
     CDS             16..753
                     /locus_tag="PLB01_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQQ77052.1"
                     /translation="MLFGSALAAGVMNALAGGGTILTFPALLLAGVPAIQANATSTVA
                     LVPGALSSLAGYRREVDTHRQWLTRLLVPSLLGGAVGSVLLLLTPEKTFARLAPYLVL
                     FATLLFIFQIVWSRREPASAAGEPASWKAAAAAQFFVAIYGGYFGAGVGILMLVILGF
                     LGLTNIHAMNGLKNFFGICINGVASAWFIIRGVVNWPLALLMMAGAIAGGWIGAGFAR
                     RIGKEKARVAVVVIGLGITVVLFLQRH"
     gene            789..1214
                     /locus_tag="PLB01_06830"
     CDS             789..1214
                     /locus_tag="PLB01_06830"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014514789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQQ77053.1"
                     /translation="MPARTASAVWTGDLKSGKGTMKFGGGAWEGAYSFQSRFEEGPGT
                     NPEELAAAAHSGCFSMAFANELSKAGFVPTRVATTAKVHLEKGESGFSITRIDLACEA
                     QVPGIDDAKFQEIGLAAKKGCPISKLFTGAQINLEAKLV"
     gene            1345..1977
                     /locus_tag="PLB01_06835"
     CDS             1345..1977
                     /locus_tag="PLB01_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HQQ77054.1"
                     /translation="MMKPRCSILTFKEISLLCLSLLVLPSAFAEDKPAAAAKPAMSQD
                     EMMKAWVAFATPGAPHKTLEMTVGTWDVKTKMWMAPGAPPQETSGTSENKMILGGRYL
                     EQRYEGTMMGQPFSGIGVTGFDNYKKKFVSTWVDSMGTGIMSMTGTADKTGKVITSWG
                     TMDDPAEKRTMKAKTVVKIVDADHHTYESWHALPDGKLTKDLEIHYTRKK"
     gene            complement(1979..3694)
                     /gene="ligD"
                     /locus_tag="PLB01_06840"
     CDS             complement(1979..3694)
                     /gene="ligD"
                     /locus_tag="PLB01_06840"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HQQ77055.1"
                     /translation="MPLEEYRKKRRFDVTPEPAGAKAPRKRGQGLVYVVQKHRATALH
                     YDFRLEWNGVLLSWAIPKGPSLDPSVKRMASHVEDHPLEYAAFEGIIPAAEYGGGTVM
                     VWDRGTWSPESPDVDAALRKGDLKFTLHGEKLKGSWVLVRTKGWGGSSKPSWLLIKHR
                     DDFASDEDVAETRPRSVVSNRLLTQIAIDEGGDVEKASTGDPVAEVEKLLANPKLLQR
                     KQKVSPAVWHSRPRGEKTGERDKEKISLKGKRAGATGAATPGRAAQPLDAPSGKSPSS
                     SPYSFPVPVSNPNKVFWPEEGWTKGDLVAFYAGVFDKLRPWVEDRPLSLERCPDGLSG
                     ECFYQKEKPPSLPPGTPTVVVRHGKDRKVTNTVVGGKLETQLALANLGCIATHVWGSR
                     ADELDKPDWVCFDLDPDSGLITDAVGAALKVKQALDALELVSYVKTSGGKGLHVFVPI
                     VRGPGTEEVTWFAKTLGTRLAAAWPKDLTMEMRIAARKGRVFLDSFRNAFGQTVVTPY
                     SVRRRPHAPVSTPLAWNEVVPSLRAEDFTIGNFAARLKKRDPWADFFRRRQALEPALE
                     ALRSL"
     gene            3832..6417
                     /locus_tag="PLB01_06845"
     CDS             3832..6417
                     /locus_tag="PLB01_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HQQ77056.1"
                     /translation="MDDTQHPVDPDVKEALLELQRYLSDSLAPLMVAESINLLLDHPA
                     ALAANEVQAWTIAQFRGRGQNVPISDYLYHAVEKLHAMGQYDLVPRDPLRRYLQDLSQ
                     QLLVFCPPADRDMLRQNLGRLGQAESVLAAPVEMLRRQGTTADAPTAMRRASGEHGVP
                     LAPQTVSDLRHLSMLIERLGPLEALPGAGGQGAPGRGEAAPFGATSGVAAPPTGAQDG
                     PAGPGSVPYGAMVQSPREALVAQILAVAAIASRSDGELGQALERLRAAGVDVPSGEVI
                     RTLGRSIPPWPLTLPPEPVEEALSSARASATQAIRRVVTLPKDQEEVGKRFHEMVAAA
                     VEQLNQGSLTRASRMFDVAEKLVVENKVSPLSLETTRKKAQDSVDLERVRSLGEDARN
                     QQPLARILSFFPGLSVGALIQELRVEEKRERRRLLLSLLEIYGPEARATALGILGQLA
                     AAGAPEHDWHFKRNLLYLIRKTPRPADQAPDAEMDAVIPFADPSLPPPIVKEAIAGLA
                     AIKHERSEAVLVTLVQGLESILAKKGDAPFDEAELQPLLDRAVAALARFGSPTARRVV
                     VEHGLKRKSGLGDVGARLAELATEDLTDDPELVSLLLKSARAEMPFKVFGLTLKKRED
                     RLSPIVEALSGTPSPGVRKVFEEIVEKHADTPAAKAAARALAAFDAPKAADEGQASLM
                     GDLAIFELPALLQSLASTEVTGALTLRDTGGGVVGKFLLENGRIRSAEAFGLKGDDAC
                     FQLFERPTGGTFSFVRQPALPPLREGESLREVVSILLEGMRRYDDFQRFSALVPDDLV
                     LAPTGTKPSTEPEEADGALQKAVWTKASGGATPKTIESAVAADSYRIRRLLVRWVEEG
                     SLKAA"
     gene            complement(6448..8457)
                     /gene="acs"
                     /locus_tag="PLB01_06850"
     CDS             complement(6448..8457)
                     /gene="acs"
                     /locus_tag="PLB01_06850"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQQ77057.1"
                     /translation="MARKQPAKKKASPAAAAPATIDVLLSEKRTFAPPKAFAAKANAK
                     SPALYAKGTKNPEAYWADWAKQLTWDKPWKKVLEWKPPHAKWFLGGKLNASVNCLDRH
                     IDGFRRTKAALVWEGEPGDRRTLTYRELYIEVNKFANVLKKFGIRRGDRVTIYLGMVP
                     ELPIAMLACARIGAIHSVVFGGFSAEALRDRINDQGAKLLITADGGWRRGNVVPLKKI
                     ADEALEDTPTIENVVLVMRTHTAIDVKEGRDHWWHRLMADAPVWCKPESMDAEDILYI
                     LYTSGTTGKPKGIVHTTGGYMTGTLATSKLVFDLKEDDIFWCTADIGWVTGHSYVVYG
                     PLANGATCIMYEGAPDTPGRDRFWDIVERHGVTILYTAPTAIRTFMKWGTEFPDKHDL
                     SSLRLLGTVGEPINPEAWIWYQKTIGKGRCPIVDTWWQTETGSILITPLPGLTKTKPG
                     SATVAFPGIEPKILDELGNEIKVGGGYLALTRPWPSMLRTLWGDDDRYVKTYFSKWDK
                     SVYFTGDGARRDKDGYFWLMGRVDDVMNVAGHRIGTMEVESALVDHPAVAEAAVVGIT
                     DEMKGTGIAAFVILKEKGRTQEAADTKSALQQEIKAHVVKKIGPIARPDKLFFTVDLP
                     KTRSGKIMRRLLRDVAEGRVLGDTTTLADPTVVKSLKEQYEEKEG"
     gene            complement(8549..9709)
                     /locus_tag="PLB01_06855"
     CDS             complement(8549..9709)
                     /locus_tag="PLB01_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77058.1"
                     /translation="MRSRILSARVALVALAVVVALLPGVASAQAIIKVNDNVNFRLGI
                     LLQGWADWNGQSDAAGNDAGYQQNLFLRRARFFLGGQVAKDVTFFFMTDNPNLGKSTQ
                     AVGSTTGVKAPGTGFIVQDAFLSWKIDNALNIQGGLILIPLCRNCNTSAVTLLSMDYG
                     TWSFQESASTQSSVGRDTGFQAMGYLAGDHLEYRAGVYSGFRAPGVKNSFRFTGRVQY
                     NVFDVEKGQFYPGTYYGKKKILAFGASYDTQSDYSAYAGDVFFDYPVGKGGITAQVDY
                     IHYDGGVTFPTAALFKQDVIFAEAGFYTGGDIKLMPFARYETQMYKNGTAGCPAAGCD
                     SLDKNFLQLGLGWFPYGANFNIKGGWTRKQFPNDPKTATTNQYTLQVQLFYY"
     gene            complement(9916..10341)
                     /locus_tag="PLB01_06860"
     CDS             complement(9916..10341)
                     /locus_tag="PLB01_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77059.1"
                     /translation="MLNLLGTNLIGILLFGGLYAAAALRYVPSWAFPPFLVAALVGFV
                     AFWIRIEKVSGTRGGVLPRLGRVLAALLLTVPAASAFILMPLFALRTHVPPEAGLDDL
                     PTRAMVLLLIALSLTVFSNLIGVLLAVVRPRVVPGRARP"
     gene            complement(10344..10631)
                     /locus_tag="PLB01_06865"
     CDS             complement(10344..10631)
                     /locus_tag="PLB01_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77060.1"
                     /translation="MREVAFRFVYLVWFLAFMATIYLALHMIVAKLIRNPQSKVLAFL
                     TIMTHPLTVPARSFLGPGTPEARVRGVTLGGLVCIWLLARTVMAFLASGLA"
     gene            complement(10704..12638)
                     /locus_tag="PLB01_06870"
     CDS             complement(10704..12638)
                     /locus_tag="PLB01_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HQQ77061.1"
                     /translation="MSLRRIFTYYTVAFLGVTVLIGLAEKFFGLPNKWIGWIFMALSL
                     GIYVVIGIVTRTSDADQYYVAGRNVPAIYNGMATGSDWMSAASFISMAGALSAQGYAG
                     LAYVMGWTGGYLLLAVLLGPYLRQFGAYTIPDFLGSRYGGNAARIVGVIAAITCSFTY
                     LIAQVTGVGLIVSRFIGLPFNVGVFVGLIGVLFCSVLGGMKSVTWTQVAQYIILIVSY
                     LVPVVYLCWTTFGIPVPQLMYGRLLQQNGVKAVEITRDPQEKATRALWKAEADDATAK
                     SKAPGISEADKEKLLAKAKTANAQAKAPASGPSDDALKANYLAVPKGVGMWNFLALTF
                     CLMVGTAGLPHILTRYYTTPSVRQARTSVGWSLFFIFLLYFTAPAYAAFARFIVYTKL
                     VGSKMTELPAWIHLWKPAGLFDVVDKAGDGVVQFADFVIKSTDFVVLATPEIAGLPFV
                     ITGLVMAGGLAAALSTADGLLLTIANAISHDLYYNVINRNASLMRRLLITKVLLVVTA
                     LIAASVATFRLAIIVELVAWAFSLAAGAFFPALVMGIWWKRANKVGAVTGMLVGFLTT
                     VFYMVGSRFYGVSWFGTTTIASGVFGIPLGFLTIWVVSLMTDAPPKEIQDLVTSVRYP
                     KSGQAITAPPDITGRGVAAH"
     gene            complement(12641..12913)
                     /locus_tag="PLB01_06875"
     CDS             complement(12641..12913)
                     /locus_tag="PLB01_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HQQ77062.1"
                     /translation="MSELTEEKRREYWSYNLRLTTLLLAIWFVVTFLISSLWAAQLNA
                     YSFLGFPLGYYMAAQGSLAIFVVEIVIYAKLMNAKDLEFGIREGDE"
     gene            complement(13217..13828)
                     /locus_tag="PLB01_06880"
     CDS             complement(13217..13828)
                     /locus_tag="PLB01_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQQ77063.1"
                     /translation="MSFLRRYFGPSPAFTDVLFWSLDLETGGLAPQRDPILAVGMVPV
                     REGTIRLAEAYSSFVRPSTAHISDSRSLQAHNLIPGDLREAPSLHDVLGEVDVRLREG
                     VLLVHFAAVDVAFLKAAYALAGRAWPSPTVVDTARLLEKLADVERLASRVREPSFNLV
                     AARRDLGLPEYPSHDALTDAVAAAELFLLLVARLGARTLRSLT"
     gene            complement(13825..15660)
                     /locus_tag="PLB01_06885"
     CDS             complement(13825..15660)
                     /locus_tag="PLB01_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HQQ77064.1"
                     /translation="MSPASVPVPPPLDPRGFLRSVPPFDALDVDAFEMAARSLDVGYF
                     PSGTRVLSQLGRPSAHLWIIRKGTVRLEKGGSAADHLEEGDLFGYASLLTGSVAFDVV
                     VQDDLLAYRIPEKVFRSLLSEPGFARFFTEGLATRLRSAVSLPGEAAFSLNVYASVGS
                     LVRRAVVSVPVTATAGDAARAMEANRVSSVLVLSDPPGIVTDRDLRNRVLAKGLGPDT
                     PVSSIATHRLTTVPADTPVYGAWHAMVGAGIHHLPVTKDGAVTGIITGTDLLKEQTFG
                     PLQLLKRVESIRDRDGLPGYGTDLARMVAALSAGGLPPTQIARLVSYLNDALVTRLLA
                     FAEADLGPAPAAWAWIVFGSEGRFEQALLTDQDNALIWDDGAGEGGAAWFARLAELVV
                     ADLVTAGFPPCPGGYMATVWRGPLAEWVARFRSWLEVPEAEAILKAAIFFDFRRAGGA
                     LDLSPISRVITQASSNAPFLSRMAGAALRFRPQLGPFRTLRDDAGRLDLKMSGIAPIV
                     GLARVYALDAGATATNTLERLAAAAESGFLPREAVATLSQAFPFLLGLRLREQLRAVA
                     SGAIPLNSIALDSLSAVERRHLKEVFLAIRDAQQDAAQRYHVSFS"
     gene            complement(15660..16016)
                     /locus_tag="PLB01_06890"
     CDS             complement(15660..16016)
                     /locus_tag="PLB01_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77065.1"
                     /translation="MGHIVSYKKDALGPRCHMKLESGERILIHSEVSTNQHATDPLWM
                     TVSTLKLGGLLPGPQLFRFDQRSDAGKKIIASLPAMKAEGAKPAPVDALVDFLKAAGS
                     VAEAKASLEKLGLPDA"
     gene            16230..>17047
                     /locus_tag="PLB01_06895"
     CDS             16230..>17047
                     /locus_tag="PLB01_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007941196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ77066.1"
                     /translation="MSTAAPAGTGERVITSAEERRVIVASSVGTVFEWYDFYLYATLA
                     PFFATLFFPKGNATAALLSAFVTYAAGFLVRPFGALIFGRVGDLVGRKYTFLVTIAVM
                     GGATAAVGFLPTFETIGWAAPFMLVILRLLQGLALGGEYGGAATYVAEHAPDHRRGYA
                     TSWIQTTATVGFFLALAVIGIARGMMKPADFSNWGWRIPFILSLVLLAISIYIRLKLQ
                     ESPIFQRLKAQGKRSKSPIKDSFFKYPNNKYAALALFGATAGQGVVWYTGQFYAL"
BASE COUNT         2810 a         5615 c         5940 g         2682 t
ORIGIN      
        1 gccgccacgc tcgcgctgct cttcggctcg gctctcgccg ccggggtcat gaacgcgctc
       61 gccggcggcg ggacgatcct gacgttcccg gcgctgctcc tcgccggcgt ccccgcgatc
      121 caggccaacg cgacctcgac ggtggccctc gtgccgggcg cgctctcgag cctcgcgggg
      181 tatcgccgcg aggtcgacac gcaccgccag tggctgacgc gccttctcgt gcccagcctc
      241 ctcggaggcg cggtgggatc ggtcctcctc ctgctgacgc ccgagaagac gttcgcacgc
      301 ctcgccccgt atctcgtcct cttcgcgacc ctgcttttca ttttccagat cgtgtggtcc
      361 cgccgcgagc cggcctccgc cgccggcgag cccgcgtcgt ggaaggcggc cgcggccgcg
      421 cagtttttcg tcgcgatcta cggcggctat ttcggggcgg gcgtcggcat cctcatgctc
      481 gtcatcctcg ggttcctcgg gctcacgaac atccacgcga tgaacggact caagaacttc
      541 ttcgggatct gcatcaacgg cgtcgcgtcc gcgtggttca tcatccgcgg cgtcgtgaac
      601 tggcccctcg ctctcctcat gatggcgggc gcgatcgcgg gcggctggat cggcgccggc
      661 ttcgcgcgcc gcatcggcaa ggaaaaggcc cgcgtcgccg tcgtcgtcat cggactcgga
      721 atcaccgtcg tactcttcct tcaacgtcac tgagcacgcc gggtttcccg gcacgaaagg
      781 agacacacat gccggcacgt actgccagcg ccgtctggac cggagacctc aagagcggaa
      841 agggcacgat gaagttcggg ggcggagcct gggaaggcgc gtactcgttc cagtcgcgct
      901 tcgaggaggg ccccgggacg aacccggaag agctcgcggc cgccgcccac tcggggtgtt
      961 tctcgatggc cttcgcgaac gagctgtcca aggcgggctt cgtcccgacg cgcgtcgcga
     1021 cgacggcgaa ggtccacctc gagaagggcg agtcgggctt ctcgatcacc cgcatcgacc
     1081 tcgcctgcga ggcgcaggtc cccgggatcg acgacgcaaa gttccaggaa atcggactgg
     1141 cggccaagaa gggctgcccg atctcgaagc tcttcacggg agcgcagatc aacctggagg
     1201 cgaaactggt ctagagggaa cgagaagatc ggcgcggcgc gccgaggaag aagattttct
     1261 tagaaggtaa agagggggag gtcgaaacag accgccccct cttcgcgtat agggggacaa
     1321 atacgcagga aaatggaggc atcaatgatg aaaccgcgct gcagcattct tactttcaaa
     1381 gaaatctctc ttctctgtct ttctctcctc gtccttccgt cggcgttcgc cgaggacaag
     1441 cccgcggcgg cggccaagcc cgccatgagc caggacgaga tgatgaaggc ctgggtcgcg
     1501 ttcgccacgc ccggcgcgcc gcacaagacg ctcgagatga cggtcgggac gtgggacgtc
     1561 aagacgaaga tgtggatggc gcccggcgcg ccgccccagg aaacgtcggg cacctccgag
     1621 aacaagatga tcctcggcgg gcgctacctc gagcagcgct acgaggggac gatgatgggg
     1681 cagcctttca gcgggatcgg cgtcacgggc ttcgacaact acaagaagaa gttcgtgtcg
     1741 acgtgggtcg actcgatggg caccgggatc atgtcgatga ccggcaccgc cgacaagacg
     1801 gggaaggtca tcacgtcctg ggggacgatg gacgatcctg ccgaaaagcg gacgatgaag
     1861 gccaagacgg tcgtcaagat cgtcgacgcc gatcaccaca cgtacgagtc ctggcacgcc
     1921 ctgcccgacg gcaagctcac gaaggacctc gagatccact acacccgaaa gaagtgattc
     1981 agagactccg gagcgcctcg agcgcgggct cgagagcctg gcggcgcctg aagaagtcgg
     2041 cccacgggtc gcgcttcttc aggcgggccg cgaagttccc gatcgtgaag tcctcggcgc
     2101 gcagggacgg cacgacttcg ttccaggcga gaggtgtcga cacaggggcg tgcgggcgcc
     2161 gccggaccga gtagggtgtc acgaccgtct ggccgaacgc gttgcggaag gagtcgagaa
     2221 agacccggcc cttccgggct gcgatccgca tctccatcgt gaggtccttc ggccaggccg
     2281 ccgcgaggcg cgttccgagc gtcttcgcga accacgtgac ctcctcggtg cccgggccgc
     2341 ggacgatcgg cacgaagacg tggagaccct tcccgccgga cgtcttcacg tacgagacaa
     2401 gctcgagggc gtcgagcgcc tgcttcacct tgagagccgc gccgacggcg tcggtgatga
     2461 gacccgagtc cgggtcgagg tcgaagcaga cccagtcggg cttgtcgagc tcgtcggcgc
     2521 ggctgcccca gacgtgggtc gcgatgcacc cgaggttcgc gagcgcgagc tgcgtctcga
     2581 gcttcccgcc cacgaccgtg ttcgtcacct tgcggtcctt gccgtggcgc acgacgacgg
     2641 tcggcgtgcc gggcggcagg ctgggtggct tttccttctg gtagaagcac tcgcccgaga
     2701 ggccgtccgg gcagcgctcg agcgagagcg gccggtcctc gacccacggg cggagcttgt
     2761 cgaagacgcc cgcgtagaac gcgacgaggt cgcccttcgt ccacccctcc tcgggccaga
     2821 agaccttgtt cgggttggag acggggacgg ggaatgaata cggggacgaa gagggggatt
     2881 tcccagaagg cgcgtcaagg ggctgcgcag cccggccagg cgtcgccgcg cctgtggccc
     2941 cggccctctt acctttcaaa gaaatcttct ctttgtccct ctctcccgtc ttttctcccc
     3001 ggggccgtga gtgccagacg gcgggggaga ccttctgttt ccgctgcagg agcttcgggt
     3061 tcgcgaggag cttctcgacc tcggcgacgg ggtcgccggt cgaggccttt tcgacgtcgc
     3121 cgccctcgtc gatcgcgatt tgcgtgagga gccggttcga gacgaccgag cgcggacgcg
     3181 tctcggcgac gtcttcgtcc gacgcgaagt cgtcacggtg cttgatgagg agccacgacg
     3241 gtttcgagga gccgccccat cccttcgtgc gaacgaggac ccaggagccc ttcagcttct
     3301 cgccgtggag cgtgaacttc aggtcgccct tcctcagggc cgcgtccacg tccggcgatt
     3361 cgggcgacca cgtgccgcgg tcccagacca tcacggtgcc gccgccgtac tccgcggccg
     3421 ggatgatgcc ctcgaacgcc gcgtactcga gcgggtggtc ctcgacgtgg gaggccatgc
     3481 gcttgacgga cgggtcgagc gagggcccct tcgggatcgc ccacgagaga aggacgccgt
     3541 tccactccag ccggaagtcg tagtggaggg ccgtcgcccg gtgcttttgg acgacgtaga
     3601 cgaggccctg cccccgcttt cgtggagcct tcgctccggc gggctccggg gtgacgtcga
     3661 accggcgctt cttccggtat tcctcgagag gcatcggggt gacgatacgg ggaacccgcg
     3721 cgggccgcca gagtcgccgc gtctgcgtgc tagcattgtc tgctctcagg acggtcgcgg
     3781 cccgggacgt ggcttgctcg ggagccattg gaggaggccg acggcggggc gatggacgac
     3841 actcaacatc cagtcgaccc ggacgtgaag gaagcgctcc tcgagcttca gcgctacctg
     3901 tcagacagcc tcgcgcctct catggtggcc gagtcgatca accttctcct cgaccatccc
     3961 gcggcgctcg ccgcgaacga ggtgcaggcc tggacgatcg cgcagttccg cgggcgggga
     4021 cagaacgttc cgatctcgga ctacctctac cacgcggtcg agaagctcca cgcgatgggt
     4081 cagtacgatc tcgtgccgcg cgatccgctg cgccggtacc tgcaggacct ctcccagcag
     4141 ctcctcgtgt tctgcccgcc ggccgaccgg gacatgctcc ggcagaacct gggccggctc
     4201 ggccaggccg agagcgtcct cgcggcgccc gtggagatgc tgcgtcggca ggggacgacg
     4261 gccgacgcgc ccacggcgat gaggcgtgcg tccggcgagc acggtgttcc tctcgcgccg
     4321 cagacggtct ccgacctgcg gcacctctcg atgctgatcg agagactcgg acccctcgaa
     4381 gcgctcccgg gggccggagg ccagggcgcc cccggaaggg gagaagccgc tcccttcgga
     4441 gcgacttccg gcgtggcggc tccgcccacg ggcgctcagg acggacccgc cggccccgga
     4501 tccgtgccct acggggcgat ggtccagtct ccgcgggaag ccctggtggc gcagatcctc
     4561 gcggtcgcgg ccatcgcctc ccgctcggac ggggagctcg ggcaggccct cgagcggctt
     4621 cgggccgcgg gcgtggacgt gccgtccggc gaggtgattc ggaccctcgg ccggtcgatc
     4681 ccgccgtggc ccctcaccct gccgccggaa cccgtcgaag aggcgctttc ctcggcgcgc
     4741 gcgtccgcga cgcaggccat ccgccgcgtc gtcacccttc ccaaggacca ggaggaggtc
     4801 gggaagcgtt tccacgagat ggtggccgcg gccgtcgagc agctgaacca gggctcgctg
     4861 acgcgcgcct cgcggatgtt cgacgtcgcc gagaagctcg tcgtcgagaa caaggtgagc
     4921 ccgctctcac tcgagaccac gcgcaagaaa gctcaggatt cggtggatct cgagcgcgtt
     4981 cggagcctcg gcgaggacgc gcgcaaccag cagcctctcg cccgtatcct ctcgttcttc
     5041 ccggggctct cggtcggagc cctcatccag gaattgcggg tcgaggagaa gcgcgagcgg
     5101 cgccgcctcc tcctctccct cctcgagatc tatgggcccg aggcgcgcgc aaccgcgctc
     5161 gggatcctcg gacagctcgc ggccgccggc gccccggagc acgactggca cttcaagagg
     5221 aaccttctct acctcatccg aaagaccccg cggccggcgg accaggcgcc ggacgcggaa
     5281 atggacgccg tcatcccgtt cgccgatccc tcgctcccgc cgcccatcgt caaagaggcg
     5341 atcgcggggc tcgcggccat caagcacgag cgctcggagg ccgtgctcgt cacgctcgtg
     5401 cagggcctcg agtcgatcct cgcgaagaag ggcgacgccc cgttcgacga ggccgagctc
     5461 cagccgcttc tcgaccgcgc ggtcgcggcg ctcgcgcggt ttggaagccc gacggcacgg
     5521 cgcgtcgtcg tcgagcacgg gctgaagagg aagagcgggc tcggcgacgt cggggcccgg
     5581 cttgccgagc tcgcgaccga ggacctgacg gacgacccgg agctcgtgag ccttctgctc
     5641 aagagcgcgc gggccgagat gccgttcaag gttttcggcc tcaccctgaa gaagcgcgag
     5701 gaccggcttt ccccgatcgt cgaggcgctg tccggcaccc cgtctcccgg cgtccggaag
     5761 gtgttcgagg agatcgtcga gaagcacgcg gatacccccg ccgccaaggc cgccgcgcgc
     5821 gccctcgcgg cgttcgacgc gccgaaggcg gccgacgagg ggcaggcgag cctcatgggc
     5881 gacctcgcga tcttcgagct tccggccctc ctgcagagcc tcgcctcgac ggaggtgacg
     5941 ggcgcgctca cgctccgtga caccggcggc ggcgtggtcg ggaagttcct gctcgagaac
     6001 gggcggatcc ggtccgcgga ggcgttcggc ctgaaggggg acgacgcctg tttccagctc
     6061 ttcgagcgtc ccaccggcgg tacgttctcg ttcgtgcgcc agccggcgct gccgccgctg
     6121 cgcgagggcg agagcctgcg cgaggtcgtg tcgatcctgc tcgaggggat gcgccgctac
     6181 gacgacttcc agcgcttctc ggcgctcgtt ccggacgacc tcgtcctcgc gccgacggga
     6241 acaaaaccgt cgacggagcc ggaggaggcg gacggcgccc tccagaaggc ggtctggacg
     6301 aaggcgtccg gcggggcgac cccgaaaacg atcgagtcgg cggtggccgc cgactcgtac
     6361 cggatccgga gactgctggt caggtgggtg gaagagggga gcctgaaggc cgcctgacgg
     6421 gcgtgacgcc ttcaagctcc ccgcgaatca gccttccttc tcttcgtact gctccttgag
     6481 cgacttgacg accgtgggat ccgcgagcgt cgtcgtgtcg ccgaggacgc ggccttccgc
     6541 gacgtcgcgc aggaggcgcc gcatgatctt gcccgagcgc gtcttcggga ggtcgaccgt
     6601 gaagaacagc ttgtcgggcc gcgcgatcgg cccgatcttc ttcacgacgt gggccttgat
     6661 ctcctgctgg agcgcggact tcgtgtcggc ggcttcctgg gtgcggccct tctccttgag
     6721 gatgacgaac gccgcgatgc ccgtgccctt catctcgtcc gtgatgccga cgaccgcggc
     6781 ctcggcgacg gccgggtggt cgacgagcgc ggactcgacc tccatcgtgc cgatccggtg
     6841 cccggcgacg ttcatgacgt cgtccacgcg gcccatgagc cagaagtagc cgtccttatc
     6901 gcggcgcgcg ccgtcgccgg tgaagtagac gctcttgtcc cacttcgaga agtacgtctt
     6961 gacgtagcgg tcgtcgtcgc cccagagcgt gcggagcatc gagggccagg gcctcgtgag
     7021 cgcgaggtag ccgccgccaa ccttgatctc gttgccgagc tcgtcgagga tcttcggctc
     7081 gatgcccggg aacgcaacgg tcgcagagcc gggtttcgtt ttcgtgaggc cggggagcgg
     7141 ggtgatgagg atcgagccgg tctccgtctg ccaccacgtg tcgacgatgg ggcagcgccc
     7201 ctttccgatg gtcttctggt accagatcca cgcctcgggg ttgatcggct ctccgacggt
     7261 gccgagcagc cggagggagg acaggtcgtg cttgtccggg aactccgtgc cccacttcat
     7321 gaaggtgcgg atcgcggtcg gggccgtgta gaggatcgtg acgccgtggc gctcgacgat
     7381 gtcccagaac cggtcgcggc ccggcgtgtc cggtgcgccc tcgtacatga tgcacgtggc
     7441 gccgttcgcg agcgggccgt agacgacgta cgagtggccc gtgacccagc cgatgtcggc
     7501 cgtgcaccag aagatgtcgt cttccttgag gtcgaagacg agcttcgatg tcgcgagcgt
     7561 gccggtcatg tagccgccgg tcgtgtgaac gatgcccttc ggcttcccgg tcgtgcccga
     7621 cgtgtagagg atgtagagga tgtcctcggc gtccatggac tcgggcttgc accagacggg
     7681 ggcgtcggcc atgaggcggt gccaccagtg gtcacggcct tccttgacgt cgatcgccgt
     7741 gtgcgtccgc atgacgagca cgacgttctc gatcgtgggc gtgtcctcga gggcctcgtc
     7801 ggcgatcttc ttgagcggga cgacgtttcc gcgccgccat ccgccgtcgg ccgtgatgag
     7861 gagcttggcg ccctggtcgt tgatgcggtc gcgcagcgcc tcggcggaga agccgccgaa
     7921 gacgacggag tggatcgcgc cgatgcgcgc acaggcgagc atcgcgatcg ggagctccgg
     7981 gaccatcccg aggtagatcg tgacgcggtc cccgcgccgg atcccgaact tcttcaggac
     8041 gttcgcgaac ttgttcacct cgatgtagag ctcgcgatag gtgagcgtcc ggcggtcgcc
     8101 cggctctccc tcccacacga gggccgcctt cgtgcgccgg aacccgtcga tgtggcggtc
     8161 caggcagttc acggaggcgt tcagcttgcc gccgaggaac cacttcgcgt gcggcggctt
     8221 ccactcgagg accttcttcc agggcttgtc ccacgtgagc tgcttcgccc agtcggccca
     8281 gtaagcctcg gggttcttcg tccccttggc gtagagcgcg ggagacttcg cgttcgcctt
     8341 ggcggcaaag gccttcggcg gggcgaacgt gcgcttctcg ctgaggagga cgtcgatcgt
     8401 ggcgggtgcg gcggcggccg gcgacgcctt cttcttcgcg ggctgtttcc gggccatggc
     8461 gtgtgctcct tctgatcttc ttttgcggag tgggccggcc gggctcacgc gggacggggg
     8521 aacccccgcc ccgcggagcc gcgcgggatc agtagtagaa gagctgaacc tggagcgtgt
     8581 actggttcgt cgtcgccgtc ttcgggtcgt tcgggaactg cttgcgcgtc cagccgcctt
     8641 tgatattgaa gttcgcgccg tacgggaacc agccgaggcc gagctggagg aagttcttgt
     8701 ccagcgaatc gcaccccgcc gccgggcagc ccgcggtgcc gttcttgtac atctgggtct
     8761 cgtaacgggc gaacggcatg agcttgatgt cgccgccggt gtagaaaccg gcttcggcga
     8821 agatcacgtc ctgcttgaag agggccgccg tcgggaacgt gacgccgccg tcgtagtgga
     8881 tgtagtcgac ctgggccgtg attccgccct tcccgaccgg atagtcgaag aagacgtcgc
     8941 ccgcgtaggc cgagtagtcg ctctgcgtgt cgtagctcgc gccgaacgcg aggatcttct
     9001 tctttccgta gtacgtgccc gggtagaact ggcccttctc gacgtcgaac acgttgtact
     9061 ggacgcgacc cgtgaaccgg aaggagttct tgaccccggg ggcgcggaag ccggagtaga
     9121 cgccggcgcg gtattcgagg tggtcgcccg cgagataccc catcgcctgg aagccggtgt
     9181 cgcgtccgac cgaagactgc gtcgaggcgc tctcctggaa ggaccacgtc ccgtagtcca
     9241 tcgagaggag cgtgaccgcg ctcgtattgc agttgcggca gagcggaatg aggatgaggc
     9301 cgccctggat gttgagcgcg ttgtcgatct tccagctgag gaaggcgtcc tggacgatga
     9361 agccggttcc gggggccttg acgccggtcg tgcttccgac ggcctgtgtg gacttcccga
     9421 ggttcgggtt gtccgtcatg aagaagaacg tgacgtcctt cgccacctgg ccgccgagga
     9481 agaatcgcgc tcggcgcagg aacaggttct gctgataccc ggcgtcgttc ccggcagcgt
     9541 ccgactggcc gttccagtcc gcccagccct gcaggaggat accgagacgg aagttcacgt
     9601 tgtcgttgac cttgatgatc gcctgggcgc ttgcgacacc gggaagcaac gccacgacga
     9661 cggccagggc gacgagggct acgcgagcgg aaaggatcct tgaacgcaca ccttcctcct
     9721 tacacgtcag cacgccgaaa gagcggcaca aacgaaagcg catcaatgtg aaaacgataa
     9781 acacccagtg gattgcgcgt caactggaat ctctgtcgag acaggatctc ttttggccct
     9841 ccacggcgct ccgatcgcgt taacaccgcc cccaccgatc gggactcccc gcggtcggcg
     9901 gccacccctc cgccctcagg gacgggcccg cccgggcacg acgcgcggcc gcacgacggc
     9961 caagaggacg ccgatcagat tcgaaaacac cgtcagagag agagcgatga gcagcaggac
    10021 cattgcgcgc gtcggcaggt cgtccaggcc ggcctcgggc gggacgtggg tgcgaagggc
    10081 gaagaggggc atcaggatga acgccgaggc ggccgggacc gtgagaagaa gcgcggccag
    10141 gacgcggccg aggcggggga gaacgccccc ccgggtgccg ctcaccttct cgatccggat
    10201 ccagaatgcg acgaagccga cgagggcggc gacgaggaac ggcgggaatg cccaggaggg
    10261 gacgtaccga agcgccgcgg cggcgtagag ccctccgaag aggaggatgc cgatcaggtt
    10321 ggtgccgagg aggttgagca tcttcaggcc agcccggacg cgaggaacgc catcacggtg
    10381 cgtgcgagga gccagatgca cacgaggccg ccgagcgtga ctccgcggac acgcgcctcc
    10441 ggcgtgccag gcccgagaaa ggagcgcgcg ggaaccgtga gcggatgggt catgatcgtc
    10501 aggaaggcga ggaccttgct ctgcgggttg cggatgagct tcgcaacgat catgtgcagc
    10561 gcgaggtaga tcgtcgccat gaaggcgaga aaccagacga ggtagacgaa gcggaaggcg
    10621 acctcacgca tcggcggagt ctagcctctg gacccggaac gggcggggcg gccgtccgcc
    10681 ccgcccgccg gtcgatcgag agctcagtgc gcggccacgc ccctccccgt gatgtccggg
    10741 ggtgccgtga tcgcctgtcc cgacttcgga tagcggacgc tcgtgacgag gtcctggatc
    10801 tccttgggcg gcgcatccgt catcagggag acgacccaaa tcgtcaggaa cccgagcggg
    10861 atcccgaaga cgcccgacgc gatcgtcgtc gtcccgaacc aggaaacccc gtagaagcgg
    10921 ctgccgacca tgtagaagac ggtcgtcagg aagccgacga gcattcccgt cacggccccg
    10981 accttgttcg cccgcttcca ccagatgccc atgacgagcg cggggaagaa cgccccggcc
    11041 gcgaggctga acgcccacgc gaccagctcg acgatgatcg cgagccggaa ggtcgcgacg
    11101 ctggccgcga tgagcgcggt cacgacgagc agcaccttcg tgatcaggag cctcctcatg
    11161 agcgaagcgt tgcggttgat gacgttgtag tagaggtcgt gcgagatcgc gttcgcgatc
    11221 gtcaggagga ggccgtccgc cgtggagagc gccgcggcga ggccgcccgc catgacgagg
    11281 ccggtgatga cgaacgggag cccggcgatc tcgggcgtcg cgaggacgac gaagtccgtg
    11341 ctcttgatga cgaagtccgc gaactggacg acgccgtcgc cggccttgtc cacgacgtcg
    11401 aagaggcccg cgggcttcca gaggtgaatc caagcgggga gctcggtcat cttcgagccg
    11461 acgagcttcg tgtacacgat gaaccgcgcg aaggcggcgt acgcgggcgc cgtgaagtac
    11521 aggaggaaga tgaagaacag cgaccagccc accgacgttc gcgcctgccg gacggacggt
    11581 gtcgtgtagt agcgcgtgag gatgtggggg aggcccgccg tcccgaccat gaggcagaac
    11641 gtgagggcga ggaagttcca catgccgacg cccttgggca cggcgaggta gttcgccttg
    11701 agcgcgtcgt cgctcggtcc cgaggccggc gccttcgcct gggcatttgc cgtcttggcc
    11761 ttggcgagga gcttttcctt gtcggcctcg gaaatgccgg gcgccttgga cttcgccgtg
    11821 gcgtcgtcgg cttcggcctt ccagagggcg cgcgtggctt tctcctgcgg gtcgcgcgtg
    11881 atctcgaccg ccttcacgcc gttctgctgg aggaggcgcc cgtacatgag ctggggcacg
    11941 gggatgccga acgtcgtcca gcagaggtag acgacgggga cgaggtacga aacgatgagg
    12001 atgatgtact gggcgacctg cgtccacgtg acggacttca tgccgccgag gaccgagcag
    12061 aagaggacgc cgatgaggcc cacgaagacg ccgacgttga acgggagccc gatgaaccgc
    12121 gacacgatga ggccgacgcc cgtcacctgt gcgatgaggt aggtgaagga acacgtgatc
    12181 gccgcgatga cgcccacgat gcgcgccgcg ttcccgccgt agcgcgaccc gaggaagtcg
    12241 ggaatcgtgt aggcgccgaa ctgccggagg tacgggccga ggagaacggc gaggagcagg
    12301 tagccgccgg tccaccccat gacgtaggcg aggcccgcgt agccctgtgc cgagagggcg
    12361 cccgccatcg agatgaacga cgccgccgac atccagtccg aaccggtcgc catgccgttg
    12421 tagatcgcag gcacgttgcg gccggcgacg tagtactggt cggcatcgga cgtgcgggtc
    12481 acgatgccga tgacgacgta gatccccagg gagagcgcca tgaagatcca gccgatccac
    12541 ttgttcggaa ggccgaagaa cttctcggcg agcccgatga ggaccgtgac cccgaggaac
    12601 gcgacggtgt agtaggtgaa gatcctgcgc agactcatgg ctactcatcc ccctcgcgga
    12661 ttccgaactc gagatccttg gcgttcatga gcttggcgta gatcacgatc tcgaccacga
    12721 agatggcgag cgagccctgg gccgccatgt agtagccgag cgggaagccc aggaacgagt
    12781 acgcgttgag ctgggccgcc cacaggctcg agatcaggaa cgtcaccacg aaccagatcg
    12841 cgagaagcag ggtcgtgagg cgcaggttgt acgaccagta ctcccggcgt ttttcttcgg
    12901 tcagctcgga catgagtccc ctccctcggt gagccggatt cggatggcgt ccatcatcaa
    12961 acgtcctcct tgttcctgaa gcgacggcag agtgcatagg cccgacacgg cggcggagcg
    13021 ccaggtacgc ccccttccgc gcattgattc aacgattcgg cccggggtgc tcaacgaact
    13081 ccctccggct ttccctgacg tagcggatga acgagcgata gcctttgaca tgtcgattcg
    13141 aatgtcaagg gcattctccc gcgggcgccc cctctttccg cggtcgggtt aacgcgccgc
    13201 gcggcgcacg cgctcgctag gtgagactgc gaagcgttct cgcgccgagt ctcgcgacga
    13261 gtaggagaaa gagctccgcg gccgcgaccg cgtccgtgag cgcgtcgtgc gacgggtact
    13321 cggggagtcc gaggtcccgg cgcgcggcga cgagattgaa cgagggctcc cggacacgcg
    13381 aggccaggcg ctcgacgtct gcgagctttt cgagaaggcg cgccgtgtcg acgacggtcg
    13441 gcgaaggcca cgcccggccg gcgagcgcgt acgccgcctt gaggaacgcg acgtccaccg
    13501 ccgcgaaatg cacgaggaga actccctccc gcaggcgcac gtcgacctcc ccgagaacgt
    13561 cgtgaagaga gggcgcctca cgcaggtctc ccgggatcag gttgtgcgcc tgaagcgagc
    13621 gggaatcgga gatgtgagcg gtggacggac ggacgaagct cgagtaggcc tcggcgaggc
    13681 ggatcgttcc ttcccggacc ggcaccatgc ccaccgcgag gatcggatcc cgctggggcg
    13741 ccaggccgcc ggtctcgagg tcgagggacc agaagagaac gtcggtgaag gccggcgacg
    13801 ggccgaagta tcgccggagg aagctcatga aaacgagacg tgataacgct gggccgcgtc
    13861 ctgctgcgcg tcccggatcg ccaggaacac ttccttgaga tgccgccgct cgaccgccga
    13921 gagcgagtcc agcgcgatgg agttcagggg gatcgcgccg gacgcgaccg cccggagctg
    13981 ttcgcggagg cgcagcccga gcaggaacgg gaacgcctgg gaaagcgtcg caaccgcttc
    14041 gcggggcagg aaacccgact cggcggccgc ggcgagccgc tcgagggtgt tcgtcgcggt
    14101 cgctcccgcg tcgagggcgt agacgcgggc gaggccgacg atgggcgcga tcccggacat
    14161 cttgaggtcc aggcggcccg cgtcgtcgcg cagcgtccgg aaggggccga gctgcggacg
    14221 gaagcggagg gcggcgcccg ccatccgcga cagaaagggc gcgttcgagc tggcctgcgt
    14281 gatgacccgc gagatcggcg agaggtccag agcgcctccc gcccgccgga agtcgaagaa
    14341 gatcgcggct ttcaggatcg cctcggcttc ggggacctcg agccagctcc ggaagcgcgc
    14401 gacccactcc gcgagcgggc ctcgccagac ggtcgccatg tacccgcccg gacacggcgg
    14461 gaagcctgcc gtgacgaggt cggcgacgac cagctcggcg agccgggcga accaggcggc
    14521 gccgccctct cctgcgccgt cgtcccagat gagcgcgttg tcctggtccg tgagaagagc
    14581 ctgctcgaac cggccttcgg atccgaagac gatccaggcc caggccgccg gcgccggacc
    14641 gagatcggcc tccgcgaagg cgaggagcct cgtcacgaga gcgtcgttga gatacgacac
    14701 gagccgcgcg atctgcgtcg gggggagccc gccggccgac agggcggcaa ccatgcgggc
    14761 gagatcggtg ccataccccg ggaggccgtc acggtcgcgg atggactcga cgcgcttgag
    14821 gagctgaagc gggccgaacg tctgctcctt gagaaggtcc gtccccgtga tgatccccgt
    14881 gacggccccg tccttcgtga cgggcaggtg gtggatcccc gcgccgacca tcgcgtgcca
    14941 ggcgccgtag accggcgtgt ccgccggaac cgtcgtgagc cggtgggtgg cgatcgacga
    15001 tacgggcgtg tcggggccga ggcccttggc gaggacccgg tttctcagat cccggtccgt
    15061 cacgatcccg ggcgggtccg acaggacgag gacggaagac acccggttcg cctccatcgc
    15121 ccgggccgcg tcgccggcgg tcgcggtgac cggcacggag acgaccgcgc ggcgcacgag
    15181 cgaaccgacc gaagcgtaga cgttgagact gaatgcggcc tcgccgggga gggacacggc
    15241 gctccggagc ctcgtcgcga gcccttccgt gaagaaacgt gcgaaacccg gctccgagag
    15301 caggctccgg aacaccttct ccgggatccg gtacgcgaga aggtcgtcct gcacgacgac
    15361 gtcgaaggcc acgctgccgg tcaggagcga cgcgtaaccg aagaggtcgc cctcctcgag
    15421 gtggtccgcg gcggacccgc ctttctcgag ccgcaccgtg cccttccgga tgatccagag
    15481 gtgcgcgctc gggcggccga gctgggacag gacgcgggtc cccgaaggga agtagcccac
    15541 gtcgagagag cgggcggcca tctcgaaggc gtcgacgtcc agcgcgtcga agggcgggac
    15601 gctgcgcagg aagcctcgcg ggtcgagagg aggcggaacg gggacgctcg ccggggacat
    15661 caggcgtcgg gcaagcccag cttctcgaga ctcgccttgg cctcggccac ggaaccggcc
    15721 gccttcagga agtcgacgag cgcgtcgacg ggagccggct tggcgccctc ggccttcatc
    15781 gccggaagcg acgcgatgat cttcttgccc gcgtcgctcc gctggtcgaa ccggaagagc
    15841 tggggtccgg gcaggagacc tccgagcttc agcgtcgaca ccgtcatcca cagggggtct
    15901 gtcgcgtgct ggttggtcga aacctcggag tggatgagga ttcgttcgcc gctctcgagc
    15961 ttcatgtgac agcgaggacc gagggcgtcc ttcttgtagc tcacgatgtg tcccatcgcc
    16021 tctcctttct cccttccgaa ctgtgtcgtc acagaatacg cctcggaccg ggaggatggg
    16081 caatggtccg tcgccggctg cgcggggaat cgcgcgatga tgcaactgga tcatagtgag
    16141 gggacgcggg cgggcccgaa catctcggcg atctcgacac gagtctgcac ggaacgcatg
    16201 ccgccgatgc cggcggacgg aggaatcgaa tgagcacagc ggcacccgcg ggcaccggag
    16261 aacgggtcat cacctccgcc gaagagcggc gcgtcatcgt cgcgtcgtct gtcggcaccg
    16321 tcttcgagtg gtacgacttc tacctgtacg cgaccctcgc tcccttcttc gcgaccctct
    16381 tcttcccgaa ggggaacgcg acggcggccc tcctctccgc cttcgtcacc tacgcggccg
    16441 gcttcctcgt gcgtcccttc ggggcgctca tcttcggccg cgtcggcgat ctcgtcggga
    16501 ggaagtacac gttcctcgtg acgatcgcgg tcatgggcgg cgccacggcg gcggtcgggt
    16561 tcctcccgac tttcgagacg atcggctggg cggcgccctt catgctcgtc atcctgcgcc
    16621 tcctgcaggg cctcgccctc ggcggcgagt acggcggcgc cgcgacgtac gtcgccgagc
    16681 atgcccccga ccaccgccgc ggatacgcga cgagctggat ccagaccacg gcgaccgtcg
    16741 gcttcttcct cgccctcgcc gtcatcggaa tcgcgcgggg catgatgaag cccgccgact
    16801 tctccaactg gggctggcgc atcccgttca tcctgtccct cgtgctcctc gcgatctcga
    16861 tctacatccg gctcaagctg caggagtcgc cgatcttcca gcgcctcaag gcgcagggga
    16921 agcgctccaa gtcgcccatc aaggacagct tcttcaagta cccgaacaac aagtacgcgg
    16981 cgctcgcgct cttcggcgcg acggccggcc agggcgtcgt ctggtacacg ggccagttct
    17041 acgcgct
//
LOCUS       DAOMFY010000058        16201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1110_length_16201_cov_7.894525, whole genome shotgun sequence.
ACCESSION   DAOMFY010000058 DAOMFY010000000
VERSION     DAOMFY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16201
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_16201_cov_7.894525"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PLB01_06900"
     CDS             complement(<1..1078)
                     /locus_tag="PLB01_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77067.1"
                     /translation="MSARDSERTALLVAALAAAPFAAALGAGFVFDDEGEVVGSVTPL
                     ATFAERLVTLGRPLLKATYALGVWAHGPSPSGFRLLNVALHAASAVLVFLLAERWLSA
                     RRPGDSGALRAAAFAGAALWALHPLAAETVTYVSGRSMGLSTLLVLASLALVAADDVP
                     STRRGLGAGLLAFLAPLAKETALVLPLLLVWWEATLGPRDVPLRERARRLAPVLGGTA
                     GAAFVLAVLPRHRELLGFSLAVRPPLEALRGNVQAVPAMLGLWAFPWRLSIDPAKPLE
                     WAWTEAPTLLGLAFLGALAAVALVARRRAPFLAFAVGWSLLALAPSNSFVWRLDPVGV
                     RPLYLASVGPAVLAAGLVGAALAAR"
     gene            complement(1075..2037)
                     /locus_tag="PLB01_06905"
     CDS             complement(1075..2037)
                     /locus_tag="PLB01_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ77068.1"
                     /translation="MKAWRVAHLLLLLAALRLSAADVVAAPKAAPPLRITLLGTGNPR
                     PMPGRYGPSILVEAATVPPTRILVDAGRGAAERLFTVGGRELLSGVDLVLLTHLHSDH
                     VVGLPDLWLTGWLFGRTKALSLKGPEGTAAMMGHLEKAFAFDVKTRRDVDERLPGAGV
                     TVEAYDVGLDTPFEVSGVRVTPFLVDHGPVKPAYGYRIDYAGKSVVLSGDTRYSENLV
                     AHAKGCDVLVHEVVAVDVERRASVMRDPAVTQRIIDHHTTPEDAGRIFAAVKPKLAVY
                     SHIVPSPTTAKDLEGPTRKTYAGPLLVGEDLTVISVGETVVVGK"
     gene            complement(2070..7760)
                     /locus_tag="PLB01_06910"
     CDS             complement(2070..7760)
                     /locus_tag="PLB01_06910"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQQ77069.1"
                     /translation="MSIRRPAGIEFLKFLVLWVGLAGAWPAAGQTYTVLYPFRASVRI
                     TSGLVEVAPGEFYGTTTSGGAWSQGSVVKVTTAGAVTTLHSFSSNIDGENPVAGLVYD
                     GAGGYLYGTTTSGGLYGRGTIFRIKPDGTSFETIYDFLAGTDPFSPQAALIKGSDGKL
                     YGTSRLGGANGLGTVFSLDVSTMPVTLTTLHSFVGGATDGAGPVASLLESGGYLYGTT
                     AEGGASGFGTVFKFDTSAGSMLWFASFDGTNGSSPRGGVVEKGGDFYGTADSGGSSGS
                     GTVFKVTSSGALTKVYDFGNGQRPFAGLTLGADGLLYGTTREGGANFHGTVFRLDPSA
                     TPVVDDTRHSFNGTDGAAPTTGLVLGSDGRLFGTTSEGGPGGYGTAYWVNPTFPTFGV
                     FYADPGTEGAGPFSPLYRDGGGVLYGTASVGGNSVGGAVYKLDCATPPCALTTLRATQ
                     DLGMGQDFSGVVPGPGGFLYGTAKDSNSIYKIDTLNGSGFQKLYTFTGTPGNFPNGSF
                     PIGVMERSGILYGATASGGASDKGTVFALDPTSPSSLAWSTPFSGTDGRYPSSQLVAG
                     SDAKLYGVTNVGGVSDKGTLFRVTTGGTHELLHSFSGPDGANPWAALTAAGPGILYGT
                     TTAGGANGKGTFFRLDATTTPATVQTLRDFTAGEVEGVSVLTLGSDGNFYGTSYGWGA
                     NRLGSVIQLAPSGALRILYSFSGTDGAFPRGGVLRLSDGTLLGTTEMAGPNDGGVVFH
                     LNPSSSSATAVVSGGGVTTCSGGNVTLTATLTGTPPWNLTWSDGFRQFVSASPATRVV
                     TPAVSTTYTLTAFSDAFGEGTVSGSGSVTVSGVAPSTVVTAPAAAPPNATGLVASVPD
                     AGVGATYAWTIANGTFTSPNNAATVTWTAGSGGFVVLSVTVTKGPTCVATGSAVTAVG
                     LTPSPSLTFTHYSGTTGGAGATDATGTASRLKTPAGLAFDESGNLYVADANAIRKVSP
                     TLEVTTFAGRTQFAGSADGSRGDARFFGARGLAVDTAAGYLYVADAGNHTIRRIDLVT
                     GAVSTYAGVAGSSGSSDGTGAGARFLYPSGLALDKARNLLFVSDRQNHTIRRIDLGTG
                     AVSTYAGVAGSTGSIDGTGAGARFLYPLDLAVDAAGSLYVTDSGNSTIRKIVPAPGNF
                     GTVTTLAGSAGAYGSDDGPLTSARFNSPRGVVSNAAGTVLHVADGFDRTIRRIDVPGN
                     AVSTIAGKANGPGGTGFEGSDDGIGDAARFVIPDALALDAAGNVWVADAGGPALRKLT
                     TPAGVVTTPVALAAALGTTDGPPRFRRPGGVVADSSGNTYVADTWNHTIRKIDSGGVV
                     TTLAGLAGTYGGANGQGSNARFLYPRSLALHGGNLYVADMNANTIRKVTTGGDVTTLA
                     GAYFQGGSDDGPGSLARFDNPRGVTVDASGNVFVADSGNSTIRMVTSSGMVSTLAGKA
                     GEGGSTDGGPTTARFNGPMGVAADASGAIYVADTYNCTIRKIVRNPSVVVSTYAGQAG
                     VCGGADGPAGSATFRLPEALAVDPAGNVWVADTSGLTIRRIDAVTGQVTTPGSLVSSV
                     ENTAGADDGTAVFARFFWPQGIAVDPSGNLLIADAGNHAIRIGRPALAVAATIDSATG
                     SVGAVRNLGSTAAATAHSWEVVRRPSGSTAALSSTSSPTPTFTPDVADLYVFRLTASD
                     GTKTSITLVSLVATSSGGCTPPPAPVITAPAIVGADSPNRTASVANNPGSIYAWTITN
                     GTITAGQGTSQITFRSGAPGTLVLAVVETNGGCSSPQATANVTVAPGGQALLFYPLYP
                     CRMYSTPGGSGASLAPGEIRTIAPAGACGIPASAKAIAANATVVGPGATGHLVVWPAG
                     TAMPVTSNLNFNGGRARSNNVVVAMPGTAATGFSIFNGSAGTTPLIIDVSGYFE"
     gene            complement(7847..8881)
                     /locus_tag="PLB01_06915"
     CDS             complement(7847..8881)
                     /locus_tag="PLB01_06915"
                     /inference="COORDINATES: protein motif:HMM:NF023431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HQQ77070.1"
                     /translation="MTTPRTVGFTVVLVSALFLTAPSSGADAPAHAVRMAGAARAFLA
                     TLAPAKAAKARYPFEAKERFDWAYVPKVRAGLPLKAMAPAQQEAALALLKAGLSEKGY
                     AKVETIRSLEGVLAGLEGSSSRDPDLYTLAVFGEPSESGIWGLRYEGHHVSLNWTFVA
                     GRSIASTPQFLGANPAEVKDGPLKGTRALAAEEDLGRAFVKALDAERRSRAVVSTSAP
                     ADILTGSSRRAATQGDGGLAWNAMTAEQQARILRLLEEHANAQRPELAAARMDRLRAA
                     GLPSIRFAWMGGLEKGQGHYYRIQGATFLVEYDDTQNGANHVHAVWRDFDGDFGEDLL
                     AEHYRNAPHR"
     gene            complement(8945..10000)
                     /locus_tag="PLB01_06920"
     CDS             complement(8945..10000)
                     /locus_tag="PLB01_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HQQ77071.1"
                     /translation="MRLVAATLALLLASPAPSAEPARDLLVRARAVLKPLPAAAPAMK
                     ENPSSPARIDLGRMLYFDPRLSSSQLISCNTCHNVSLAGADLQETSTGHGWQRGPRNA
                     PTVFNAVFNVAQFWDGRAEDLKTQAKGPVQASVEMNNDPSRLVATLKSIPGYAPAFRK
                     AFPREDDPITFDNVAKAIEVFEATLLTPDAPFDRYLKGDAKALTPSEKAGLALFIDKG
                     CATCHDGVNVGGADYDPFGVVEMPPDDVRPPGDVGRFKVTNTAADTYVFRAAPLRNVA
                     VTQPYFHSGKVWKLEDAVKIMGSAQLGHTLTDAEAASVTIFLRTLTGRQPRVPNPVLP
                     PSSDATPQPKLEVKPPA"
     gene            10117..10548
                     /locus_tag="PLB01_06925"
     CDS             10117..10548
                     /locus_tag="PLB01_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77072.1"
                     /translation="MRRALVAATLLAGCLAPAARSTAGDLGAPGQRPTSPAAGYACIS
                     SPRAPQTKVVLGAPPAGFTCPKGQQLFIVTRQLQDKVAAAPATEIDVPGQAGPPGVSP
                     LPGSQEQEVRAKKRRISRKCTSDGTGGQYCCYGGTSNCQLE"
     gene            complement(10616..11389)
                     /locus_tag="PLB01_06930"
     CDS             complement(10616..11389)
                     /locus_tag="PLB01_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HQQ77073.1"
                     /translation="MRLTLNVWRQKDAKSEGKFVTYDAPDISPDMSFLEMLDVVNERM
                     ILKGEDPIAFDHDCREGICGMCGIVVNGNPHGPRRGTTACQLHMRTFKDGDAITLEPW
                     RATPFPIVKDLIVDRSSYDRIIAAGGFISVATGSAPDGNAIPVKKSCSDRAMDAAACI
                     GCGACAAACPNGSAMLFVAAKVSHLALLPQGQPERYDRVLSMVAQMDAEGFGTCTNHG
                     ECSRACPKEIKQEFIAQLNRDFFKATLMSRPKVKEAGSG"
     gene            complement(11386..13299)
                     /locus_tag="PLB01_06935"
     CDS             complement(11386..13299)
                     /locus_tag="PLB01_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HQQ77074.1"
                     /translation="MELKSNVPDGPLENKWDKHRFDMKLINPANKRKYSVIVVGSGLA
                     GGAGAASLAELGYNVSCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSIRRLFYDTVK
                     GGDFRAREANVYRLAQVSVNIIDQCVAQGVPFGREYGGLLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALERQIGLGKVKMYPRHEMLELVVIDGRARGIVTRDMVAGTIQSHI
                     ADAVVLATGGYGNVFYLATYAKGCNGTAIWRAYKKGAAFGNPCYTQIHPTCIPVTGDH
                     QSKLTLMSESLRNDGRIWVPLKKGDKRAAADIPEAERDYYLERKYPSFGNLAPRDIAS
                     RAAKQVCDEGRGIGETGFGVYLDFADAISRLGEDTIRERYGNLFDMYERITDENPYKV
                     PMRIYPASHYTMGGLWVDYNLMSTIPGLHVAGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIGNYLAGTKKDSVDTKHAAFRDAEAAVSQRMKKLLDVKGKRTVDSFHRELGKI
                     MWEFCGMGRNKAGLETALQRIPALREEFWKNVTVVGGAGELNQSLEKAGRVADFLELG
                     ELMCLDALAREESCGGHFREEYQTPDGEALRNDEKFCHVAAWEYTGEGKTPVRHVEQL
                     EFQNVHLATRSYK"
     gene            complement(13299..13967)
                     /locus_tag="PLB01_06940"
     CDS             complement(13299..13967)
                     /locus_tag="PLB01_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HQQ77075.1"
                     /translation="MAAPATLLNSSVGKKIVMAITGIVLSLFVLGHMAGNLQAFLPNG
                     PEALDHYGAFLRELLHGTGIWFVRAGLLLAVGLHAWAYLTLTQKSRAARPKGYKVNDF
                     EEATFASRSMRWTGPILGAFIVFHLMHLTIGNVHPHFVEGKVYQNLVTGLAPVPVALF
                     YVLAMGCLAFHLWHGAWSMLQTLGLSHPKYLGARKAFAVLFTILVAGGFVLVPLAVLA
                     GVLK"
     gene            complement(14004..14927)
                     /gene="mdh"
                     /locus_tag="PLB01_06945"
     CDS             complement(14004..14927)
                     /gene="mdh"
                     /locus_tag="PLB01_06945"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQQ77076.1"
                     /translation="MRRKVTVIGAGNVGATCAQRLVEQNIADVVLVDVIEGVPQGKGL
                     DMFQSGAVEGFDMKIVGTNGYEETEGSDLVIVTAGLARKPGMSRDDLLKKNEEIVASC
                     MRTTRGSRDLKVIVVTNPLDAMCEVVRRITKLPKQNVIGMAGILDSARMRAFISMELG
                     VSMSNIHATVLGGHGDTMVPLPRYSTVAGVPITELMSPERVAAIVKRTAGGGAEIVNF
                     LKTGSAYYAPSSAAVEMAEAIFNDRKKILPCAAYLEGEYGINGLFVGVPVVLGAGGIE
                     KIIELKLTPEESAALKKSAASVQELVGLLTV"
     gene            15104..15466
                     /locus_tag="PLB01_06950"
     CDS             15104..15466
                     /locus_tag="PLB01_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQQ77077.1"
                     /translation="MAGEYKNLPLWKRGMTLAHTVYAAVEAAGAKGTEAGTRLRKAAV
                     SVPSLVGEAFLDLSGKDVGEALSLAEAKLDEVQTLLRSPSLSGIPAADLESLLGDLEG
                     LKAELVQLRASRTGETTH"
     gene            complement(15463..>16201)
                     /locus_tag="PLB01_06955"
     CDS             complement(15463..>16201)
                     /locus_tag="PLB01_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ77078.1"
                     /translation="SAAGLSPVFETYERPAGAAVDAPARNVLADIPGTDGARGLVLLA
                     AHLDGVAVGEGAADNAVNVAVLLEAARTLKALAPAPRRTIRFAFFTGEEQKMAGSSAY
                     VAAHAAEPHALAVVFDLGSGRTKGFYMNGRKEDLRSLFRRALAPDDRWRAVFGTYEIW
                     AGCDAFPFVKAGVPVLTAYQIDPGYAALHHTAADTAGRLDFAAARETAALAAALAWGV
                     ADDAGPDLPRHTPDEVRELLARHRIAP"
BASE COUNT         2453 a         5545 c         5766 g         2437 t
ORIGIN      
        1 cccgcgccgc gagcgcggcg ccgacgagcc ccgcggcgag gaccgccggc ccgacggacg
       61 cgaggtagag aggcctcacg ccgacggggt ccagcctcca gacgaacgaa ttgctcggcg
      121 cgagcgcgag aagcgaccag ccgaccgcga aggcgaggaa cggcgcgcgc cttcgcgcga
      181 cgagcgcgac cgcggcgagc gctccgagga acgcgagccc gagaagcgtc ggcgcttcgg
      241 tccaggccca ctcgagaggc ttcgccgggt cgatgctgag gcgccacggg aaggcccaga
      301 ggcccagcat cgccgggacg gcctgaacgt tgccgcgcag cgcctcgagc ggcgggcgca
      361 ccgcgaggct gaatccgaga agctcgcggt gccgaggcag gacggcgaga acgaacgcgg
      421 caccggccgt tccgccaagg acgggcgcga ggcggcgcgc gcgttcgcgc aaaggcacgt
      481 cgcgcggccc gagcgtcgcc tcccaccaga cgaggagcag cggcaggacg agcgccgttt
      541 ccttcgcgag cggcgcgagg aacgcgagga gacccgcgcc gaggccgcgc cgcgtggacg
      601 gaacgtcgtc cgcggcgacg agcgccaggg acgccaggac gaggagcgtc gagaggccca
      661 tcgagcggcc cgaaacgtac gtcacggtct cggccgcgag cgggtgcagc gcccaaagcg
      721 cggcgcccgc gaacgcggcc gcccgcagcg cgccggaatc gccgggacgc cgtgcggaga
      781 gccaccgctc ggcgagaagg aagacgagga cggccgacgc ggcgtggagc gcgacgttga
      841 gaagccgaaa gcctgagggt gacggaccgt gcgcccagac gccgagcgca tacgtcgctt
      901 tcaggagcgg ccggccgaga gtcacgaggc gctccgcgaa cgtcgcgagc ggggtgacgc
      961 ttccgacgac ctcaccctcg tcgtcgaaca cgaaccccgc gccgagcgcg gccgcgaacg
     1021 gcgccgccgc gagcgcggcg acgaggagcg cggtccgctc cgagtcccgc gcgctcactt
     1081 ccccacgacc acggtctccc cgaccgagat gaccgtcagg tcctccccga cgagcagcgg
     1141 ccccgcgtag gtcttccgcg tcgggccctc gaggtccttc gccgtcgtgg gcgacgggac
     1201 gatgtgcgag tagaccgcga gcttcggctt cacggcggcg aagatccttc cggcgtcttc
     1261 gggcgtcgtg tgatggtcga tgatgcgctg cgtgaccgcg ggatccctca tcacgctcgc
     1321 gcggcgctcg acgtcgacgg cgacgacctc gtggacgagg acgtcgcaac ccttcgcgtg
     1381 cgcgaccagg ttctccgaat agcgcgtgtc cccggagaga acgacggact tccccgcgta
     1441 gtcgatccgg tagccgtagg cgggcttcac cggcccgtga tcgaccagaa agggagtgac
     1501 gcgaacgccg gagacttcga agggggtgtc gagacccacg tcgtacgctt caacggtcac
     1561 ccccgcaccg ggcaacctct cgtcaacgtc tcttctcgtc ttcacgtcga atgcaaaggc
     1621 cttctcgagg tgtcccatca tggccgcggt cccttccgga cccttcaacg aaagcgcctt
     1681 cgtcctgccg aacagccagc ccgtcagcca caggtccggc agtccgacga cgtggtcgga
     1741 atggaggtgc gtcaggagga cgagatcgac gcccgagagc agctcccgcc cgcccacggt
     1801 gaagaggcgc tcggccgcgc cgcgcccggc gtcgacgagg atgcgcgtcg gcgggaccgt
     1861 cgccgcctcg acgaggatgg acgggccgta ccgcccgggc atcggccgcg gattgcccgt
     1921 gccgagaagc gtgattcgca gcggcggagc ggccttcggc gccgccacga cgtcggccgc
     1981 ggaaagacga agcgcggcga gcagcaagag gaggtgcgcc acccgccacg ctttcatgcg
     2041 ccgagtctat tcgttcgccc gcacgggcct cactcgaagt agcccgacac gtcgatgatg
     2101 aggggcgtcg tcccggccga gccgttgaag atcgagaacc ccgtcgcggc cgtgcccggc
     2161 atcgcgacga ccacgttgtt ggagcgcgcc cgtcccccgt tgaagttgag gttggacgtc
     2221 acaggcatcg ccgtcccggc aggccacacc acgaggtgtc ccgttgcgcc gggcccaacg
     2281 accgtcgcgt tggccgcgat cgccttcgcc gacgccggga tcccgcaggc gcctgccggc
     2341 gcgatcgtgc ggatctcacc gggcgcgagc gacgcgccgc tcccgcccgg agtggagtac
     2401 atgcggcacg ggtagagggg gtagaagagc agggcctgcc cgcccggcgc gaccgtcacg
     2461 ttcgccgtcg cctggggcga cgagcagccg ccgttcgtct cgacgacggc cagaacgagc
     2521 gtccccggcg cgcccgaccg gaacgtgatc tggctcgtgc cctgccccgc cgtgatcgtc
     2581 ccgttcgtga tcgtccaggc gtagatcgac ccggggttgt tcgcgacgct ggcggtccgg
     2641 ttcggcgagt ccgcgccgac gatcgccggc gccgtgatga ccggcgccgg tggcggcgtg
     2701 catcccccgg aagaggtcgc aacgagcgag acgagcgtaa tgctcgtctt cgtcccgtcg
     2761 ctcgccgtga ggcggaagac gtagaggtcc gcgacgtccg gcgtgaacgt cggggtgggg
     2821 ctggacgtcg aggaaagcgc cgccgtcgac cccgagggcc gccggacgac ctcccacgaa
     2881 tgcgccgtcg ccgcggccgt cgagccgaga ttccgcacgg cgccgacgct gccggtggcc
     2941 gagtcgatcg tggcggcgac ggcgagcgcg ggccggccga tccggatcgc gtggttcccg
     3001 gcgtcggcga tcaggaggtt ccccgacggg tcgacggcga tcccctgagg ccagaagaac
     3061 cgggcgaaga ccgccgttcc atcgtcggcg ccggccgtgt tctcgaccga gctcacgaga
     3121 cttccgggcg tcgtgacctg acccgtgacc gcgtcgatcc gccggatcgt caggccgctc
     3181 gtgtccgcga cccacacgtt tccggcggga tccaccgcga gggcttccgg gagcctgaac
     3241 gtggcgctgc ccgccgggcc gtccgctccg ccgcagacac cggcctggcc agcgtatgtc
     3301 gagacgacaa ccgacgggtt ccggacgatc ttgcggatcg tgcagttgta cgtgtccgcc
     3361 acgtagatgg cgccggacgc gtccgcggca acacccatcg gcccattgaa ccgcgcggtc
     3421 gtcggcccgc cgtcggtcga ccccccttcc ccggccttgc cggcgagcgt cgacaccatg
     3481 cccgacgacg tcaccatccg gatcgtggag ttccccgaat ccgcgacgaa cacgttgccc
     3541 gaggcgtcga ccgtgacgcc cctcggattg tcgaaacgcg cgaggcttcc cggaccgtca
     3601 tcgctgccgc cctggaagta ggcgccggcc agcgtcgtga cgtcgccccc ggtcgtcacc
     3661 ttccggatcg tgtttgcgtt catatcggcg acgtagagat tcccgccgtg aagggcgagg
     3721 gaccgcgggt agaggaaccg cgcattgctc ccctgcccgt ttgcacctcc gtacgtcccc
     3781 gcgaggccgg ccagcgtcgt gacgacaccc cccgaatcga tcttgcggat cgtgtgattc
     3841 cacgtgtcgg cgacgtaggt gtttccggac gaatccgcga cgacaccccc cggcctccgg
     3901 aaccttggcg gaccatccgt ggtgccgagc gcggctgcaa gggcgaccgg cgtcgtcacg
     3961 acgccggcag gggtcgtgag cttgcgcagg gccgggccgc cggcgtccgc aacccagacg
     4021 ttcccggcgg cgtccagcgc gagggcgtcc ggaatgacga agcgcgccgc gtctccgatg
     4081 ccgtcgtcgc ttccctcgaa gcccgtgccc ccggggccat tggccttgcc ggcgatggtg
     4141 gagacggcgt tgcccgggac gtcgatccgc cggatcgtcc ggtcgaagcc gtccgccacg
     4201 tgaagcacgg tgccggcggc gttcgaaacc actcccctcg gactgttgaa ccttgctgac
     4261 gtcagagggc cgtcgtcgct cccgtaggcg cccgcacttc cggcaagcgt cgtgaccgtt
     4321 ccgaagttgc ctggggccgg aacgatcttc cggatcgtgg agtttcccga gtcggtcacg
     4381 tagaggctcc ccgccgcgtc caccgcgagg tccagcggat acaggaagcg cgcgcccgct
     4441 ccggtgccgt caatgctccc ggtcgagccc gccacgccgg catacgtcga gacggccccg
     4501 gtcccgaggt cgatcctccg gatcgtgtgg ttttgacggt cggaaacgaa aaggaggttc
     4561 cgcgctttgt cgagcgcgag gccggacgga tacaggaagc gcgcgcccgc tccggtgccg
     4621 tcactgctcc cgctcgagcc cgccacgccg gcgtacgtcg agacggcccc ggtcacgagg
     4681 tcgatcctcc ggatcgtgtg gttcccggca tcggccacgt agaggtaccc cgcggcggta
     4741 tccacggcga ggccgcgagc cccgaagaac ctcgcatcgc cccgcgagcc gtccgccgag
     4801 cccgcgaatt gcgtgcggcc cgcgaaggtc gtcacctcga gggtcggcga gactttccgg
     4861 atcgcgttgg cgtcggcgac gtagaggttg cccgattcgt cgaaagcgag gcccgcgggg
     4921 gttttcaggc gtgacgccgt gcccgtcgcg tccgtcgcgc ccgcgccacc cgtcgttccg
     4981 gagtaatgcg tgaacgtcag agacggagag ggggtgaggc ccacggccgt aacggccgag
     5041 ccggtcgcca cgcacgtcgg gcctttggtg accgtgacgg agaggacgac gaacccgccg
     5101 ctcccggccg tccacgtgac cgtcgccgcg ttattcggcg acgtgaacgt cccgtttgcg
     5161 atcgtccagg cgtacgtcgc gccgacgccg gcgtcgggca cggaggcgac gaggccggtg
     5221 gcgttcggcg gagccgcggc cggcgccgtg acgaccgtcg acggggcgac gcccgaaact
     5281 gtaaccgacc cgctccccga gacggtccct tctccgaaag catccgagaa ggcggtcagc
     5341 gtgtaggtcg tcgagacggc tggtgtcacg acccgcgtcg ccgggctggc cgagacgaac
     5401 tgccgaaagc cgtcggacca ggtgaggttc cagggcggcg ttcccgtcag tgtcgccgtg
     5461 agcgtgacgt tgcccccgct gcacgtcgtg actccgccgc ccgagacgac ggccgtggcc
     5521 gacgagctcg agggattcag atggaagacg acgccgccgt cattcggccc cgccatctcg
     5581 gtggtgccga ggagcgtccc gtcgctgagc cgaaggacgc ctccgcgcgg gaaagcgccg
     5641 tcagtccccg agaaggagta aaggatccgg agcgcgccgc tgggggccag ctggatgacc
     5701 gaaccgaggc ggttcgcgcc ccagccgtac gaggtcccgt agaagttccc gtcgctcccg
     5761 agggtcagga cggaaactcc ttccacctcg cccgctgtaa agtcgcgaag cgtctggacg
     5821 gtcgcgggcg tcgtcgtcgc atcgagacgg aagaacgtcc ccttgccgtt cgcgccgccg
     5881 gccgtcgtgg tcccgtagag gatgcccggc cccgcagccg tgagcgcggc ccagggattg
     5941 gcgccgtccg gtcccgagaa ggaatggagc agctcgtgcg tgccgccggt cgtcacccgg
     6001 aagagagtgc ccttgtcgga gacgccgccg acattggtca ctccgtagag ctttgcgtcg
     6061 ctgcccgcga cgagctggga cgacgggtat cggccgtcgg tcccggagaa aggagtggac
     6121 caggcgaggc tcgaggggct cgtggggtcc agcgcgaaga ccgtcccctt gtccgacgca
     6181 ccgcccgagg cggtggcgcc gtagaggatt ccgctgcgct ccatgacgcc gatcgggaag
     6241 ctcccgttcg ggaagttccc gggcgtcccg gtgaaggtgt agagcttctg gaagccggag
     6301 ccgttcagcg tgtcgatctt gtagatgctg ttgctgtcct tggccgtgcc gtagaggaag
     6361 ccgccggggc ccggcacgac gcccgagaag tcctgtccca tgccgaggtc ctgcgtggcc
     6421 cggagcgtcg tgagggcgca ggggggagtc gcgcagtcga gcttgtagac cgcgccgccc
     6481 acgctgtttc cgccgacgga cgccgtgccg tacagaacgc cgccgccgtc ccggtagagc
     6541 ggcgagaacg ggcccgcgcc ctccgtcccc gggtcggcgt agaagacgcc gaacgtcggg
     6601 aaggtcgggt tcacccagta cgccgtgccg tagccccccg ggcccccttc cgacgtggtc
     6661 ccgaaaagcc tcccgtcgga tcccaatacg agaccggtcg tcggcgccgc tccgtcagtt
     6721 ccattgaaag agtggcgagt gtcgtcgacg acgggcgtcg cggacggatc gagccggaag
     6781 accgtcccgt ggaagttcgc tccgccctcg cgcgtcgtcc cgtagaggag cccgtcggcg
     6841 ccgagggtga gcccggcgaa cgggcgctgg ccgttgccga agtcgtagac cttcgtcagc
     6901 gcgccggagc tcgtcacctt gaacacggtg cccgagcccg aggacccgcc cgaatcggcc
     6961 gtgccgtaga aatcgccccc cttttcgacg acgcctcccc gcggggaact gccattcgtc
     7021 ccgtcaaacg acgcgaacca caacatcgag cccgcggaag tgtcgaactt gaagaccgtt
     7081 ccgaaaccgg acgcgccccc ctcggcggtg gtcccgtaga ggtacccgcc gctctcgagg
     7141 aggctcgcga ccgggccggc tccgtccgtc gcgccgccga cgaacgaatg gagcgtcgtc
     7201 agcgttacgg gcatcgtcga gacgtcgagc gagaagacgg tcccgaggcc gttcgccccg
     7261 ccgaggcgcg acgtcccgta cagcttcccg tcactcccct tgatgagggc cgcctggggg
     7321 ctgaaagggt cggtcccggc gaggaagtcg tagatcgtct cgaacgacgt cccgtcgggc
     7381 ttgatccgga agatggtgcc tctaccgtaa aggccaccgc tcgtcgtcgt cccgtagagg
     7441 tagccgcccg cgccgtcgta gacgaggcca gccacggggt tctcgccgtc tatgttgctg
     7501 ctgaacgaat ggagcgtggt cacggccccg gctgtcgtca ccttgacgac ggagccctgc
     7561 gaccaggcgc cgcccgaggt cgtcgtcccg tagaactctc ccggggccac ctcgacgagg
     7621 ccggaggtga tcctcacgga cgcccggaag ggatagagga ccgtgtacgt ttgcccggcg
     7681 gctggccacg cgccggcaag gccgacccaa aggacgagga acttcaggaa ctcgattccg
     7741 gccgggcgac ggatggacat ggtctcctcc tcgagggggg ctcagaggag gagaagcgca
     7801 atccgccttt tttttccctc agggcggagg gaccccggga accggactaa cggtgcggag
     7861 cgttccggta gtgctcggcg aggaggtcct ctccgaaatc gccgtcgaag tcccgccaga
     7921 cggcgtggac gtggttcgcg ccgttctgcg tgtcgtcgta ttcgacgagg aacgtggccc
     7981 cctggatgcg gtagtagtgc ccttgcccct tctcgaggcc gcccatccac gcgaaccgga
     8041 tcgacgggag gcccgcggcc ctgaggcggt ccatccgggc ggcggcgagc tcggggcgct
     8101 gcgcgttcgc gtgctcctcg agcagacgca ggatccgagc ctgctgctcc gccgtcatcg
     8161 cgttccacgc gagcccgccg tccccctgcg tggccgcccg gcgggaactg cccgtgagga
     8221 tgtccgccgg cgccgacgtc gagacgacgg ccctgcttct gcgttcggcg tcgagcgctt
     8281 tcacgaaggc acgtccgagg tcctcctcgg ccgcgagggc gcgcgtcccc ttcaggggcc
     8341 cgtccttcac ctccgcgggg ttcgcgccga ggaactgggg agtcgaggcg atcgaacggc
     8401 ccgcgacgaa cgtccagttc agcgacacgt ggtggccctc gtagcgaagg ccccagatgc
     8461 cggactcgga cggctcgccg aagacggcaa gggtgtagag gtcgggatcg cggctggacg
     8521 acccttcgag gcccgcgagg acgccctcga gcgagcggat ggtctcgacc ttggcatagc
     8581 ccttctcact gaggcccgcc ttcaggagcg cgagggcggc ctcctgctgc gccggcgcca
     8641 tcgccttgag cggaaggccc gcccgcacct tcgggacgta cgcccagtcg aagcgttcct
     8701 tcgcctcgaa cgggtacctc gccttcgcgg ccttggcggg cgcgagcgtc gcgagaaacg
     8761 cccgggccgc tccggccatc cgcacggcgt gcgcgggagc gtcggccccc gaggagggag
     8821 ccgtgagaaa gagagcgctc acgagaacga cggtgaaccc gaccgtgcgt ggtgtcgtca
     8881 tgcgacggga ttctaggggt gaccccgctt cgcaccccct gtgcgcctct cggaagaaaa
     8941 acgctcaggc cgggggcttc acctcgagct tcggctgcgg cgtcgcgtcg gaagagggcg
     9001 gcaggacggg gttcgggacc ctgggctgcc gcccggtgag tgtcctcagg aagatcgtca
     9061 cgctcgcggc ttccgcatcc gtcagcgtat ggccgagctg ggccgagccc atgatcttca
     9121 ccgcgtcctc gagcttccat accttgccgg aatggaagta gggctgcgtg acggcgacgt
     9181 tgcgcagggg agccgcccgg aagacgtacg tgtccgcggc cgtgttcgtg accttgaagc
     9241 ggccgacgtc tcccggcggg cggacgtcgt cgggcggcat ctcgacgacg ccgaacgggt
     9301 cgtagtcggc cccgccgacg ttgacgccgt cgtggcacgt cgcgcagccc ttgtcgatga
     9361 agagggcgag ccccgccttc tcggacggcg tgagggcctt cgcgtcgccc ttcaggtaac
     9421 ggtcgaacgg cgcgtcgggc gtcaggagcg tggcctcgaa gacctcgatg gccttcgcga
     9481 cgttgtcgaa cgtgatcggg tcgtcttcgc gcggaaacgc cttgcggaat gcgggcgcgt
     9541 agccggggat gctcttcagc gtcgcgacga gacgggacgg gtcgttgttc atctcgacgg
     9601 aggcctgcac gggccctttc gcctgcgtct tcaggtcctc ggcccggccg tcccagaact
     9661 gcgcgacgtt gaagacggcg ttgaacacgg tcggagcgtt gcgcgggccc ctctgccagc
     9721 cgtggcccgt cgacgtctcc tggaggtcgg cgcccgcgag gctcacgttg tggcacgtgt
     9781 tgcagctgat gagctgcgag gacgaaaggc gcgggtcgaa gtagagcatc cgtccgaggt
     9841 cgatccgggc gggactggac gggttttcct tcatggccgg ggccgcagcc ggcagcggct
     9901 tgagcacggc acgggcccgg acgaggagat cgcgtgcggg ttccgcggac ggggccgggg
     9961 acgcgagcaa gagcgcaagg gtggcggcga cgagtctcat gggagctcct cccgtcccgg
    10021 aatctaggtc cgcccccccg gagccgccgc caccggcggt ctccccatga cccccggcgc
    10081 cgggcgtttg cgccttccct ggcaaaggag actcccatga gacgtgcact ggtcgccgcc
    10141 acgctcctcg caggatgcct cgccccagcc gcccgctcca ccgccggtga cctgggcgct
    10201 cccgggcaga ggccgacgtc acctgccgcc ggctacgcct gcatctcgag cccgcgcgcg
    10261 ccgcagacga aagtcgtcct cggggcgcca ccggccggct ttacctgccc gaaggggcag
    10321 cagctcttca tcgtgacgag acaattgcag gacaaggtcg cggccgcgcc cgcgaccgag
    10381 atcgacgttc ccggccaggc gggtccgccg ggggtgtcgc ctctcccggg ctcgcaggag
    10441 caggaggtcc gtgcgaagaa gagaaggatc tcgaggaaat gcacgtcgga cggcaccggc
    10501 gggcaatact gctgctacgg cggaacctcg aactgccagc tcgaataggg cgggaacgaa
    10561 cgcggcgggc gaccgaagcc gcccgccgcg cctggggccg tgaatcaggg tgactctagc
    10621 cgctgccggc ttccttcacc ttcggtctcg acatgagcgt cgccttgaag aagtcgcggt
    10681 tcagctgggc gatgaactcc tgcttgatct ccttcgggca ggcgcgcgag cactcgccgt
    10741 ggttcgtgca ggtgccgaag ccctcggcgt ccatctgcgc gaccatcgag agcacgcggt
    10801 cgtagcgctc gggctgcccc tgcgggagca gcgcgaggtg cgagaccttg gccgccacga
    10861 agagcatcgc cgacccgttc gggcaggccg cggcgcaggc gccgcacccg atgcaggccg
    10921 ccgcgtccat cgcgcggtcc gagcagctct tcttgacggg aatcgcgttg ccgtcggggg
    10981 cgctgcccgt cgcgacggag atgaagccgc ccgccgcgat gatccggtcg tacgacgagc
    11041 ggtcgacgat gaggtccttg acgatcggaa acggcgtcgc ccgccagggc tcgagcgtga
    11101 tggcgtcgcc gtccttgaag gtgcgcatgt ggagctggca ggccgtcgtg ccgcggcgcg
    11161 ggccgtgggg gttgccgttc acgacgatgc cgcacatgcc gcagatgccc tcgcggcagt
    11221 cgtggtcgaa cgcgatcggg tcttcgccct taaggatcat gcgctcgttc acgacgtcga
    11281 gcatctcgag gaaggacatg tccggactga tgtccggggc gtcgtacgtg acgaacttcc
    11341 cttcggactt cgcgtccttc tggcgccaga cgttcagcgt cagtctcatt tgtagctccg
    11401 cgtcgcgagg tggacgttct ggaactcgag ctgctcgacg tgtcggaccg gcgtcttgcc
    11461 ttcgcccgtg tactcccagg ccgccacgtg gcagaacttc tcgtcgttgc ggagggcctc
    11521 gccgtcggga gtctggtact cctcgcggaa gtggccgccg caggactcct cgcgcgcgag
    11581 cgcgtcgagg cacatgagct cgccgagctc gaggaagtcg gccacgcggc cggccttttc
    11641 gagcgactgg ttgagctcgc cggctccgcc gacgaccgtc acgttcttcc agaactcctc
    11701 gcgcagggcg gggatcctct ggagcgcggt ctcgaggccg gccttgttgc ggcccatgcc
    11761 gcagaactcc cacatgatct tgccgagctc gcggtggaag gagtcgaccg tgcgtttgcc
    11821 cttcacgtcg aggagcttct tcatgcgctg cgagacggcc gcctcggcgt cccggaaggc
    11881 cgcgtgtttc gtgtcgacgg agtccttctt ggttccggcg aggtagttgc cgatcgtgta
    11941 cggaaggacg aagtagccgt ccgcgaggcc ctgcatgagc gcgctcgcgc cgaggcggtt
    12001 cgcgccgtgg tcggagaagt tcgcttcgcc ggccacgtgg aggcccggga tcgtggacat
    12061 gaggttgtag tccacccaga gcccgcccat cgtgtagtgc gaggccgggt agatgcgcat
    12121 cgggaccttg tacgggttct cgtcggtgat gcgctcgtac atgtcgaaca ggttgccgta
    12181 gcgctcgcgg atcgtgtcct cgccgaggcg gctgatcgcg tccgcgaagt cgaggtagac
    12241 cccgaagccc gtctcgccga tgccgcggcc ctcgtcgcag acctgcttgg cggcgcgcga
    12301 ggcgatgtcg cgcggcgcga ggttgccgaa cgaggggtac ttccgctcga ggtagtagtc
    12361 gcgctcggcc tcgggaatgt cggccgcggc gcgcttgtcg cccttcttga gggggaccca
    12421 gatgcgcccg tcgttgcgga gcgactcgga catgagcgtg agcttggact ggtggtcgcc
    12481 ggtgaccggg atgcacgtcg ggtggatctg cgtgtagcag gggttcccga acgcggcgcc
    12541 cttcttgtac gcgcgccaga tggccgtgcc gttgcagccc ttcgcgtacg tcgcgaggta
    12601 gaagacgttg ccgtagccgc cggtcgcgag gaccacggca tcagcgatat gggactgtat
    12661 tgttccggcg accatgtcgc gcgtcacgat gccgcgggcg cgtccgtcga tcacgacgag
    12721 ttcgagcatc tcgtggcgcg ggtacatctt gaccttgccg agaccgatct ggcgctcgag
    12781 ggcctggtag gcgccgagga gcagctgctg ccccgtctgg ccccgcgcgt agaacgtccg
    12841 cgagacctgc gcgccgccga acgagcggtt cgcgaggagg ccgccatact cgcgcccgaa
    12901 cgggacgccc tgcgcgacgc actggtcgat gatgttcacg gagacctgcg cgagccggta
    12961 gacgttcgcc tcgcgggccc ggaagtcccc gcccttgacc gtgtcgtaga agaggcgccg
    13021 gatgctgtcg ccgtcgttct ggtagttctt cgcggcgttg atgccgccct gcgcggcgat
    13081 cgagtgcgcg cggcgcgggc tgtcctggta gcagaagcag gagacgttgt acccgagctc
    13141 ggcgagggac gcggcacccg ctccgcccgc gaggcccgac ccgacgacga tcacggagta
    13201 cttccgcttg ttcgccgggt tgatgagctt catgtcgaag cggtgcttgt cccacttgtt
    13261 ctcgagggga ccgtcgggga cgttcgactt gagttcgatc atttcagcac ccccgccagg
    13321 accgcgagtg gcacgaggac gaaccctccc gcgacgagaa tcgtgaacag gacggcgaag
    13381 gccttgcgcg ccccgaggta cttcgggtgc gagaggccga gcgtctggag catcgaccac
    13441 gcgccgtgcc agaggtggaa cgcgaggcag cccatcgcga ggacgtagaa gagcgcgacc
    13501 gggaccggcg cgaggcccgt gacgaggttc tggtagacct tgccctcgac gaagtgcggg
    13561 tgcacgttgc cgatcgtcag gtgcatgagg tggaacacga tgaaggcgcc gaggatgggc
    13621 cccgtccagc gcatggagcg cgaggcgaag gtcgcttcct cgaagtcgtt caccttgtag
    13681 cccttcgggc gggccgcccg gctcttctgc gtgagcgtca ggtacgccca cgcgtggagg
    13741 cccaccgcga ggaggagccc cgcccggacg aaccagatgc ccgtgccgtg cagcagctcg
    13801 cgcaggaacg cgccgtagtg gtcgagcgcc tccggaccgt tcggcaggaa cgcctggagg
    13861 ttgccggcca tgtggccgag gacgaagagc gacaggacga tccccgtgat cgccatgacg
    13921 atcttcttgc cgaccgagga attcaggaga gttgcgggcg cagccattcg agcggttctc
    13981 ctcccctcag gtgggtggcg ggatcagacg gtcagcagtc cgacgagctc ctggacggag
    14041 gccgcggact tcttgagagc ggcggactcc tccggcgtga gcttgagctc gatgatcttc
    14101 tcgatgcctc cggcgcccag gacgacgggc acgcccacga agagcccgtt gatgccgtac
    14161 tcgccctcga ggtacgcggc gcacgggagg atcttcttgc ggtcgttgaa gatggcctcg
    14221 gccatctcga cggccgccga ggacggcgcg tagtacgccg agccggtctt gaggaagttc
    14281 acgatctcgg cgccgccgcc ggcggtgcgc ttcacgatcg cggcgacgcg ctcgggcgac
    14341 atgagctcgg tgatcgggac gcccgcgacg gttgagtaac gcggcagcgg aaccatcgta
    14401 tctccgtgtc cgccgagaac ggtcgcgtgg atgttcgaca tcgagacgcc gagctccatc
    14461 gagatgaacg cgcgcatgcg ggcgctgtcg aggatgcccg ccatgccgat cacgttctgc
    14521 ttcgggagct tcgtgatgcg gcggacgacc tcgcacatcg cgtcgagcgg gttcgtgacg
    14581 acgatgacct tgaggtcgcg cgagccgcgc gtcgtccgca tgcagctcgc cacgatctcc
    14641 tcgttcttct tgaggaggtc gtcgcgcgac atgcccggct tgcgggcgag gccggccgtc
    14701 acgatcacga ggtcagaccc ttcggtctcc tcgtagccgt tcgtgccgac gatcttcatg
    14761 tcgaagcctt cgacggcgcc ggactggaac atgtccaggc ccttgccctg cggcacgccc
    14821 tcgatgacgt ccacgagcac gacgtcggcg atgttctgct ccaccaagcg ctgggcgcac
    14881 gtcgctccga cgtttccagc cccgatcacg gtcactttgc gtcgcatgga tggttccttc
    14941 cccggcgcct ctcgtttttt ttcggcggtg ccgtcggcga ggcagcctag caccgggtaa
    15001 ataggcctca tatgaagcaa acccatcagt cagagggtgc ttggacggcc cccaggaggg
    15061 gatcccggaa cgtccggacg cccccgggcg tataaggggt gtgatggccg gcgaatacaa
    15121 gaacctcccg ctctggaagc gagggatgac cctcgcccac acggtctacg cggccgtgga
    15181 agcggccggg gccaagggga cggaggccgg cacgcgcctt cgaaaagcgg ccgtttccgt
    15241 gccttccctc gtcggcgagg ccttcctcga cttgtccggc aaggacgtcg gggaggccct
    15301 ctcgctggcc gaggcgaagc tggacgaggt ccagacgctc ctgaggtcgc cgtccctgag
    15361 cggcatcccg gccgccgacc tcgagagtct tctcggcgac ctcgagggcc tgaaggccga
    15421 gctcgtgcag cttcgggcca gccggacggg cgaaacgaca cactaaggcg cgatccggtg
    15481 ccgcgccagg agctcccgga cctcgtccgg cgtgtgccgc gggaggtccg gcccggcgtc
    15541 gtccgcgacg ccccacgcga gggcggccgc cagcgccgcc gtctcgcgag ccgccgcgaa
    15601 gtcgagccgg ccggccgtgt ccgccgccgt gtggtggagg gccgcgtagc ccgggtcgat
    15661 ctggtacgcc gtgaggaccg ggacgccggc cttcacgaag ggaaaggcgt cgcagcccgc
    15721 ccagatctcg tacgtgccga agacggcccg ccagcggtcg tcgggggcga gcgcgcgccg
    15781 gaagagggag cggagatcct ccttgcgccc gttcatgtag aagcccttcg tccggccgga
    15841 accgagatcg aaaaccacgg cgagggcgtg cggctcggcg gcgtgggctg ccacgtaggc
    15901 cgacgagccc gccatcttct gttcctcccc ggtgaagaat gcgaaccgga tcgtccggcg
    15961 cggcgcgggg gcgagcgcct tcagcgtgcg cgccgcctcg agaagcaccg ccacgttgac
    16021 ggcgttgtcc gccgccccct cccccaccgc gacgccgtcg agatgggccg cgaggaggac
    16081 gagcccgcgc gcgccgtccg ttccgggaat gtccgccagg acgttgcgcg ccggcgcgtc
    16141 gacggcggcg cccgccgggc gctcgtacgt ctcgaagacg ggcgagaggc cggccgccga
    16201 a
//
LOCUS       DAOMFY010000059        16190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1111_length_16190_cov_7.647350, whole genome shotgun sequence.
ACCESSION   DAOMFY010000059 DAOMFY010000000
VERSION     DAOMFY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16190
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1111_length_16190_cov_7.647350"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLB01_06960"
     CDS             complement(<1..1077)
                     /locus_tag="PLB01_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77079.1"
                     /translation="MAAAVERNRLLAELAEFCAAHSVDEKILLVPSFPVGQQILDALA
                     RSGCSHVNLRPATVFSLAHGVAGPALAAAGRETLSRAQLLALVEDACDQVLRPDSYFG
                     ALRERPGLHRALQSTLNEIRRAGLTPEQLPNEALEDPRKAREMKAIGRAYRAALEAGS
                     FADPLDVLAAATRMLGENPALKLGPAARVLKPESLELSSLEAAFLDAFLPRPATALAV
                     DTAQDTPLTPERVSLACASGEENEVRAVFRRIVDEEIPLDDVELAVTDDAAYRPLVYE
                     LAQQAGFPCTFADGIPATFSRPAQGVLDVLDWIAGGYEERPLARLFGAGRANLAAAHP
                     RGSLVGPVRAARAFRRARIVRGRER"
     gene            1123..>1418
                     /locus_tag="PLB01_06965"
     CDS             1123..>1418
                     /locus_tag="PLB01_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQQ77080.1"
                     /translation="MEGISLGVPTLVAIAVYLTAINVLGAWLGRGQKDAKDYFLGGHA
                     MPWGAVMASIVATETSALTFLSVPGDAYRSGFTFLQLAFGYLVGRIAVSFILLPG"
     assembly_gap    1419..1518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1519..2226
                     /locus_tag="PLB01_06970"
     CDS             <1519..2226
                     /locus_tag="PLB01_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQQ77081.1"
                     /translation="ACRGLRDAQKALIGSGVLVILQFTLFLALGVGLFAFYGGTPVGP
                     GGFPSSDAILPTFIVKSLPPFVSAYLVAGIFSAAMCSESSALNSLASALSLDIVAPLM
                     GKKYVEGRRGLVLGKVLTLFWTLVLAGLAVGFSRLPQNVPAVQVALGLASVTAGGLLG
                     GFLLALWARKATQTDAMIGIATSAVFMFVLWLGSKGFVPFPLGKMIAWPWYSLLGSTI
                     AFGTGWLLSLRRTAVSA"
     gene            2385..2837
                     /locus_tag="PLB01_06975"
     CDS             2385..2837
                     /locus_tag="PLB01_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77082.1"
                     /translation="MKTDKMPLSWMACALAILTSAGSNEALGQSQQFYSLTPCRVVDT
                     RPGAAIPAATERQFQIKALCGVPGDAKTVTLNVTAVTPSQDGFVTLWPAGGVYTGISI
                     INVIAGQAPVANGAVMKLGTGTLDLSAAYGTQGGGTIHLVIDVTGYFK"
     gene            2869..5604
                     /locus_tag="PLB01_06980"
     CDS             2869..5604
                     /locus_tag="PLB01_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQQ77083.1"
                     /translation="MRFWPAIHALSFLMAATAPADVFVVRARPGAALSVEASLSTKAV
                     GEKFCKPLHCIVTTRVPLETAALNALRVAGAVELEPLPDAHKVMFANAIFDGDAGTYS
                     KDFPGKAELPTTGSSALYAVMFKALPEAEWLNEIIALGGVPVAFMPSMSYLVYGPRSL
                     KAKLASKDYVFRVEEAPAGIKRSGLDDAGMGGSDEIQTLVSIVNVSSSNVKDRISKSH
                     GGTPPSARKDGFLETLEARMTHSEALALSRLPEVMFIARSREAKPSDERSNRIIAGGQ
                     PGSGSGSWPPAIGTNLGFWSTYLANLSTIPTFNLSNQVIGFLDTGVDAGMKGCGDRCP
                     PYLRANASDTCRLVYTNDVTSDFSSYGIQGGDPASNRRATDYDYHGTLTTSIAAGFAG
                     SESSGVDTNGYAFTQGVAQGARVAMSKIFAPACDPNANATFWRTFGDVEGQFAPSKLN
                     QLIRYSFVALTRQTPLPDGSPAGPGARLFNHSWNASTAGYDDVAATMDTASLSGLSFT
                     FLYSDGHQEALSSGYPMVRPSLHIVAAGNCAPSPCNPAVDSVLSPATAKNVIAVGATY
                     SDNHQPFMPLCAACNELAPGTDYRTVSPWSRSGNLGGRIKPDLVAPGVRSYGRRSSSI
                     FYCASDGCNSSLGNYPGPANSYKWSTGTSFAAPVVTGAAVVVSEWLDALVRNGSVDST
                     YWPPDSYGVVYPTPSLIKAILIGGAKDLCPTDSCPIPKLNSSDPDRLMRSAPDRFQGW
                     GGVSLERLFGAASSYFLGNRMTIRGLGVWSKTLSVVDGSRPITVSLVWPDPTSAVGPL
                     VDNVLVADLDLTVSFTGRDGVARTYYGNLYGTPSNPLVRDGYSLPNPSAPTFDRAVNN
                     VERVSIRVAEAPAAGSSLTVTVTPFTLGPPACDPTGYSCVQRAFQDFSLFADNAH"
     gene            5687..6490
                     /locus_tag="PLB01_06985"
     CDS             5687..6490
                     /locus_tag="PLB01_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77084.1"
                     /translation="MPLVGVLSQIIPGPQGPTYVYWTSSTGVFNNGPSPAMVHVKAAY
                     GGGLWIASPPPCLNYQLRAGGGSSLGGCVFGPPGDGGNGFIEIAVEPSIVVTADIQRL
                     LNVPNCVIGSIDGYGLISQGSAPLPVYRGFFSAESTVVAGPVDLGSFDGPGTCITGPA
                     THRRRVNVTLFNGGTSEATFTVTAIPLRASSAPIYETHADVPPRDVRQLNAIPIPVSS
                     DLPYEFGYDIRVWLRITATQPFLAYVSTIFEGGEPGTNAVTVYEAKTSN"
     gene            6500..7612
                     /locus_tag="PLB01_06990"
     CDS             6500..7612
                     /locus_tag="PLB01_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HQQ77085.1"
                     /translation="MKNGLLPRRLGMRRLALDVVATIIAVTVGFAAPAQVDLALGNYP
                     SNSSCVILGAKIAFELRIFSSGNASGVILRDKLPIGTSFVSATRAPFSVCNDEVMWSI
                     PTVSSAGDSVTVVARVDAVPPSSYLLNLASVFGNESDYAEGNNSFASPLIRVVTTPDA
                     GCLSVVALQPGAFLSSGGQPMSILGNGFQSNATVTIAGSQLGGASFISSSQINGASPA
                     LPPGQYDLTLTNPGGAKATLIGGFNVSDPIPLGFVSVTPCRVFDTRDASGSPSLGGNT
                     SRSFLVAGRCGVPTGAKAIALNVTIVPTGGGGSLALYAGDMAPPLASFLSFDPGSVRA
                     NNGFYPLATNGSGTLGIVNNSISAVDVVLDAFGYFQ"
     gene            complement(7629..8063)
                     /locus_tag="PLB01_06995"
     CDS             complement(7629..8063)
                     /locus_tag="PLB01_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HQQ77086.1"
                     /translation="MGEEKILSESNRADGQGAADAATAAQSAVPSDAPSKQSSSSPSR
                     SPLEQKVIDEALKKCFDPEIPVNIWELGLIYSVAASPEGVVDVKMTLTAPACPVAGSL
                     PGEVEAKVKAVPGVTDAKVELVWDPPWNADMMSRVARVMLGM"
     gene            complement(8077..8529)
                     /locus_tag="PLB01_07000"
     CDS             complement(8077..8529)
                     /locus_tag="PLB01_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HQQ77087.1"
                     /translation="MTDLRDLYQEVILDHNKSPHNYREMTDPSRMALGHNPLCGDRLK
                     LYIRLDGDRIADVAFQGSGCAISKASASLMTDAVKGRTLEEAEALYGKFHDLLTGPPD
                     EKATGAGLGKLAVFSGVREFPARVKCATLAWHTLESALKNVHEVAKTE"
     gene            complement(8529..9788)
                     /locus_tag="PLB01_07005"
     CDS             complement(8529..9788)
                     /locus_tag="PLB01_07005"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQQ77088.1"
                     /translation="MNVVPETALPGVSPAFDVEAIREQFPILRQTVHGRKLVYLDNAN
                     TTQKPRAVIDADRKYYEETNANIHRGTHFLSEKATAEYEGARAKVARFLGARAPREVV
                     FTRGTTEAINLVAAGFAQSVLKEGDEILLTGLEHHSNIVPWQLACGRTGAKLVVVPIT
                     DSGEVPVEEFEKRLSDRTRIAAVSHVSNALGTINAVEEFVRLAHERDVPVLVDGAQAA
                     PHLPIDVAALGCDFYALSGHKMYGPTGIGALYGTEAWLEKLPPYQGGGDMISSVTFEK
                     TTYNELPWKFEAGTANIAGGIGFGVAADWLTGVGLPAVAAHEHALLARATEALTAIEG
                     LRIVGTAPRKAAVVSFLLGEVHPHDIGTILDREGIAIRTGQHCAQPVMDRYGIGATAR
                     ASFGVYNTVEEVDVLAAALKKVQEVFA"
     gene            complement(9785..11149)
                     /gene="sufD"
                     /locus_tag="PLB01_07010"
     CDS             complement(9785..11149)
                     /gene="sufD"
                     /locus_tag="PLB01_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HQQ77089.1"
                     /translation="MTPSARPSTIRDGVDVFATQAVEFASGLAGPPWLTRLRRDGVAS
                     FVKAGFPSLRDEEWRYTNLLPLARTVFGLAPRDVTIAERTQAVELEARLGTLGLSSPG
                     PRLVFVNGRLVRRTSHTLPPEILFENLATVLRLAPERVEKALVAAPSAHPFVALNDAF
                     LDTGALVHVGKGLTAPAPLVVVHLSTSASGTPAPLMSHLRNLVVLEENAAATVVEVFL
                     GTDEMSFTNAVTQAVVGENARLTHVKVQLEGRLAFHVGNLFLSHARGARSASHVYSFG
                     GATVRNEIAATLSGEGSGTELYGLFAAAAGQNVDCHTVIDHAAPRADSLELYKGILDG
                     NGRGAFDGKVIVREGAVKTDARQTNRNLILSNDALVDSKPQLVIHNNDVRCTHGSTTG
                     RIDADALFYLRSRGLSEGAARALLTYAFGSEVVSKVAAPDVKALLEDRLHAWLPAAAA
                     AVNA"
     gene            complement(11152..11940)
                     /gene="sufC"
                     /locus_tag="PLB01_07015"
     CDS             complement(11152..11940)
                     /gene="sufC"
                     /locus_tag="PLB01_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQQ77090.1"
                     /translation="MSNVNTPLLEIRNLHASIEEDGTEILRGIDLKILPGEVHAVMGP
                     NGSGKSTLAHVLSGRPGYAVTQGTVSFLGKDLLAMKPEERAREGVFLAFQYPVEIPGV
                     SNNYFLRTAVNAVRRHRGLAELDAIDFLALLDEKAKLVEMEEALLNRSVNEGFSGGEK
                     KRNEVLQMALLDPRLAILDETDSGLDIDALRIAAGGVNALRDGKRGMLVITHYQRLLN
                     YIVPDFVHVLSDGRIVKSGDKSLALELEAKGYGWIEADAAAAAR"
     gene            complement(11996..13438)
                     /gene="sufB"
                     /locus_tag="PLB01_07020"
     CDS             complement(11996..13438)
                     /gene="sufB"
                     /locus_tag="PLB01_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HQQ77091.1"
                     /translation="MSDALLNELTQREYQHGFVTEVETESIPAGLSEDVVRIISKKKN
                     EPEWLLEWRLKAYRHWLTMEEPTWARVSYPPIDYQKIVYYAAPKSAKEGPKSLADVDP
                     ELLAMYEKLGVPLKEREILAGVAVDAVIDSVSVATTFREKLASVGIIFCSFSEAVHTH
                     PDLVRKWLGSVVPSRDNFFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINQADTGQ
                     FERTLIVAEEGATVSYLEGCTAPKRDVNQLHAAVVELVALDDATIKYSTVQNWYPGDR
                     EGKGGIYNFVTKRGKCLGKRSKISWTQVETGSAITWKYPSCILMGDDSVGEFYSVALA
                     NNYQQADTGTKMIHIGKNTRSTIVSKGISAGHGQNTYRGGVTILRSAEGARNYSQCDS
                     LLIGDQCGAHTFPYIEVKNSSATLEHEASTSKIGEDQLFYCMQRGISAEDAVSLIVNG
                     FAKRVLKELPMEFAVEAQKLLGVSLEGSVG"
     gene            complement(13435..13896)
                     /locus_tag="PLB01_07025"
     CDS             complement(13435..13896)
                     /locus_tag="PLB01_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HQQ77092.1"
                     /translation="MFRMTKMTDYGIVLLTQFAAHEDRPAFTARELAHDTRLPLPTVG
                     KLLKQLSHGGLLASQRGTRGGYSLARPPREITVASIIATLEGPVSITECGGAVRCEHE
                     SFCSTKPNWQIINDAIRASLEGLTLADMTRPLPPRFAAASAAALPAHRSPA"
     gene            complement(14089..14934)
                     /gene="amrB"
                     /locus_tag="PLB01_07030"
     CDS             complement(14089..14934)
                     /gene="amrB"
                     /locus_tag="PLB01_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQQ77093.1"
                     /translation="MKTNRTGAPRVRPPAVAGMFYPASPAALARHVDALLAGAVAAPE
                     EPLPLALVVPHAGTIYSGPVAASAYARLRPHAAMIDRVVAFGPVHRVPVHGAAVPAAD
                     AWATPLGEVPIDAALRMRAAACGAAVDDRPHAPEHALEVQLPFLQRVLGDRFTFLPVA
                     VSEISSRATADLIAEFLGEKGTVVVVSTDLSHYHDQETARRLDRMTADAILAREPDNL
                     SGEDACGYYPLRGLLELARRRSLPVKLLDLRTSGDTAGDPVSVVGYGAFEVLPPKTGE
                     KSRIS"
     gene            complement(14931..16013)
                     /gene="amrS"
                     /locus_tag="PLB01_07035"
     CDS             complement(14931..16013)
                     /gene="amrS"
                     /locus_tag="PLB01_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQQ77094.1"
                     /translation="MTAPEAFHPTKYWHAEGDRVQCDVCPRFCRLKDGQEGHCFVRAA
                     RGGEIVLTTYGRSSGFCVDPIEKKPLNHFLPGTSVLSFGTAGCNLACKFCQNWDISKS
                     REMDTLAADATPARIAAAAVEYGCRSVAFTYNDPVVFMEYAVDVAAACRAAGVKTVAV
                     TAGYVEPNPRAELFAAMDAANVDLKAFTEDFYAKTCGAHLQPVLETLEYLVRETKVWT
                     EITTLLIPGMNDSESEIDAMTRWVLEHLGPDVPHHFTAFHPDWKMRDASATPAATLKK
                     AREIAKKNGLNYVYTGNVHDPQGQSTDCPRCGERVIGRDGYEIFEWSLAKGGVCASCG
                     APCPGAFEEVPGSWGSRRLPVHLAAS"
BASE COUNT         2523 a         5202 c         5507 g         2858 t
ORIGIN      
        1 tcgctcgcgg ccccgcacga tccgcgcgcg gcggaaggcc cgcgccgccc ggaccggccc
       61 gacgagggag ccgcgggggt gcgcggccgc gagattcgcg cgtcccgccc cgaagaggcg
      121 ggcgagaggt cgctcttcgt agccgccggc gatccagtcc agcacgtcga ggacgccctg
      181 ggccgggcgc gaaaacgtgg ccgggatccc gtcggcaaac gtgcacggga agcccgcctg
      241 ctgggcgagc tcgtagacga gcggacggta cgcggcgtcg tccgtgaccg cgagttcgac
      301 gtcgtcgagc gggatctcct cgtccacgat tctgcggaag accgcgcgca cctcgttctc
      361 ctcccccgac gcgcacgcca gcgacacgcg ctccggcgtc aggggtgtgt cctgtgccgt
      421 gtccacggcg agggccgtgg ccggacgcgg caggaacgcg tcgaggaacg cggcctcgag
      481 cgacgagagc tcgaggcttt ccggcttgag gacccgggcc gcgggcccga gcttcagggc
      541 cgggttctcg ccgagcatcc gcgtcgcggc ggcgaggacg tcgagcgggt ccgcgaagct
      601 cccggcctcg agtgcggcgc ggtaggcgcg gccgatggct ttcatctccc gcgccttccg
      661 cgggtcctcg agcgcctcgt tcgggagctg ctcgggcgtg aggcccgcgc ggcggatctc
      721 gttcagcgtg gactggaggg cgcggtggag gccgggtcgt tcgcgcagcg cgccgaagta
      781 cgaatcgggc ctcaggacct ggtcgcaggc gtcctcgacg agcgcgagga gctgcgcgcg
      841 cgagagcgtc tcgcgcccgg ccgccgcgag agccggcccg gcgacgccgt gcgcgaggga
      901 aaagacggtc gcgggccgca ggttcacgtg agagcagccg gagcgggcga gcgcgtcgag
      961 gatctgctgg ccgaccggga atgacgggac gaggagaatc ttctcgtcca cggaatgcgc
     1021 cgcgcagaat tccgccagct ccgcgaggag cctgttgcgt tcgacggcgg cggccacgcg
     1081 cggagtctaa atgggccggg ccctgtgtga tcatgccggc gcatggaagg catctccctc
     1141 ggagtgccga cgctcgtcgc gatcgccgtg tacctcacgg ccatcaacgt gctcggcgcc
     1201 tggctcggca ggggccagaa ggacgcgaag gactatttcc tgggcggcca cgcgatgccg
     1261 tggggggccg tcatggcttc catcgtcgcg acggagacgt ccgcgctcac gttcctctcc
     1321 gtgcccggcg acgcataccg ctcggggttc acgttcctgc agctcgcgtt cggctacctc
     1381 gtcgggcgca tcgccgtgtc gttcatcctc ctgccgggnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnngc gtgccgcggc ctgcgcgacg cgcagaaggc gctcatcggc
     1561 tcgggcgtcc tcgtgatcct gcagttcacg ctgttcctcg cgcttggcgt cgggctcttc
     1621 gcgttctacg gcgggacgcc cgtcgggccc gggggcttcc cgtcctcgga cgcgatcctg
     1681 ccgacgttca tcgtgaagag cctgccgccg ttcgtgtcgg cgtacctcgt cgcggggatc
     1741 ttctcggccg cgatgtgctc ggagtcgtcg gcgctcaact ccctcgcgtc cgcgctctcc
     1801 ctagacatcg tcgcgccgct gatggggaag aagtacgtcg aggggcgccg tggcctggtg
     1861 ctcgggaagg tgctgacgct cttctggacg ctcgtcctcg cgggcctcgc ggtggggttc
     1921 tcgcgcctgc ctcagaacgt accggccgtt caggtcgcgc tcgggctcgc gagcgtgacg
     1981 gcgggaggcc tcctcggtgg cttcctcctc gcgctctggg cgcggaaggc gacgcagacg
     2041 gacgcgatga tcggcatcgc gacgtctgcg gtcttcatgt tcgtcctgtg gctgggctcg
     2101 aagggcttcg tcccgttccc gctcgggaag atgatcgcgt ggccgtggta ctcgctcctc
     2161 ggctcgacga tcgcgttcgg gacgggctgg ctcctgtcgc tgaggcgaac ggccgtctcg
     2221 gcctagattc gctagcgggt cctaactggt gagcgtgtaa cccgatcggg ttagtacgac
     2281 cgcatcatgg cgctgccaag tacacgcacc tacaagtgaa gagtgtcctc cacgggcgag
     2341 gctgggcgca gcaaccgact tagagcggta gaaacaggtc agcaatgaaa acagacaaaa
     2401 tgcctctttc gtggatggca tgtgcacttg ccattctaac tagcgcggga agcaacgagg
     2461 cgctcggcca aagccagcag ttctacagcc tgacgccttg cagggttgtt gatacgcgcc
     2521 ctggggccgc cattccagcc gcgaccgagc ggcagttcca gattaaagcg ctctgcggtg
     2581 taccgggaga cgccaagacg gtgacgctaa acgtcacggc tgtaaccccg agccaagacg
     2641 ggttcgtcac gctctggcct gccggtggcg tctacaccgg gatctcgatc atcaatgtca
     2701 tcgcaggcca ggcgcccgtt gccaacggtg ccgtgatgaa actcggcaca ggaactctgg
     2761 acctttcggc ggcttacggc acgcagggcg ggggaacgat ccacctcgtc attgacgtta
     2821 ccgggtactt caagtaacgc cgccgcggag attcagatgc accaccgcat gagattctgg
     2881 cctgccatcc acgcgctgtc gttcctcatg gctgcaacag cgccggccga cgtgtttgtc
     2941 gttcgggccc gtccgggcgc cgcactgtcc gtggaagcgt ctctttccac gaaggccgtg
     3001 ggcgagaagt tctgtaagcc tcttcactgt atcgtcacta caagggtgcc cctcgaaacg
     3061 gcagccctca atgccttgag agtagcgggc gccgtcgagc tcgaacccct gccggacgcg
     3121 cacaaggtga tgttcgcgaa cgcgatcttc gacggcgacg caggcacgta cagcaaggac
     3181 ttccccggta aggccgagct acccacgacg ggctccagcg ctctgtacgc ggtgatgttt
     3241 aaggcactcc cggaggcaga gtggctgaat gagatcatcg cgctcggcgg ggtacccgtc
     3301 gccttcatgc cttccatgag ctatcttgtc tatgggcccc gaagtctcaa agcgaaactc
     3361 gcgagcaagg actacgtgtt tcgcgtcgag gaggcaccgg ccgggatcaa gcgttcgggc
     3421 ctcgacgacg ctggcatggg cggaagcgac gagatccaga ccctggtttc gatcgttaac
     3481 gtctcatctt cgaacgtgaa ggacagaatt tcgaaaagcc acggcgggac accgccgagc
     3541 gcccggaaag acggattcct cgaaacgctc gaagcgagga tgacgcactc ggaagcgctc
     3601 gccctcagcc gcttgcccga ggtgatgttc atcgcccgga gccgcgaagc gaagccctca
     3661 gacgagaggt cgaaccggat catcgcgggc ggccagccag gctccgggtc cggctcgtgg
     3721 ccgccggcga tcggcacgaa tttgggcttc tggagcacct acctcgccaa cctgtcgacc
     3781 atcccaacgt tcaacctctc gaaccaggtc atcggctttc tggatacggg cgtggacgcc
     3841 ggaatgaaag gctgcgggga tcgctgtccg ccctaccttc gcgcgaacgc atcggacacg
     3901 tgccgtctgg tctacaccaa cgacgtcacg tcggacttca gcagctacgg cattcagggc
     3961 ggggaccccg cgagcaaccg gcgagctaca gactatgact accacggcac cctgacgacc
     4021 tcgatagcgg ctggctttgc ggggtctgag agctcggggg tcgatacgaa tggctacgcc
     4081 ttcacccagg gcgtcgctca gggtgcgcgg gtggccatgt cgaagatctt cgcgcccgca
     4141 tgtgacccga acgcgaacgc tacgttctgg cggacgttcg gtgacgtcga ggggcaattc
     4201 gcgccttcca aactgaatca actgattcgg tattcctttg tggctttgac gcgccagacg
     4261 cccctgccag atggttcccc tgcaggaccc ggcgcgcgac tgttcaatca cagctggaat
     4321 gcgtcaaccg caggttacga cgacgtcgca gcaacgatgg acactgcctc tctctcgggc
     4381 ctctcgttca ccttcctgta ctcggatggg catcaagagg ccctttccag tggctacccg
     4441 atggtccggc cttcgctgca catcgtggct gcaggcaatt gcgccccctc cccgtgcaat
     4501 cctgcggtcg actctgtcct ctctcccgcg accgcgaaga acgtcatagc ggtcggcgct
     4561 acttattcag acaaccatca gccgttcatg ccgctctgcg ccgcgtgtaa cgagcttgct
     4621 ccgggtaccg actaccggac cgtttcaccg tggagccggt ccggtaacct cggcggccgc
     4681 atcaagccgg atctggtggc gcccggcgtt agatcgtatg gccgtcgctc cagctcgatc
     4741 ttctattgtg cgtcggacgg ctgtaactcg agtcttggaa attacccagg ccccgcaaat
     4801 tcctacaagt ggtctacggg cacgagtttt gccgccccag ttgtgaccgg tgccgcggtg
     4861 gtcgtcagtg aatggctgga tgcgctggtg aggaacggaa gcgttgacag cacctactgg
     4921 ccgccggatt cctacggcgt cgtgtatcca accccgagcc taatcaaagc aatcctgatc
     4981 gggggggcca aagacctctg cccaaccgac agctgcccta ttccaaagct caactcatct
     5041 gaccccgatc gtctcatgcg ttcagcgcca gatcgcttcc aggggtgggg tggcgtgagc
     5101 ctcgagcgtc tcttcggcgc cgcgagcagc tacttcctcg gaaatagaat gacgattcgc
     5161 ggccttgggg tgtggagtaa gacgctcagc gtcgtcgacg gcagccgtcc aataacagtc
     5221 tcgctcgttt ggcccgatcc aacgagcgcc gtaggacctc ttgtcgacaa cgtgcttgtg
     5281 gcggatctcg acctcacggt ctccttcaca ggtcgtgacg gtgtggcccg aacgtattac
     5341 gggaaccttt acggcacgcc gtcgaatccg ctcgttcgcg atgggtattc gctcccgaat
     5401 ccatctgctc cgactttcga tagggcggtc aacaacgtcg aacgagtcag catccgcgtg
     5461 gccgaggctc ctgcggccgg tagcagcctg acggtcacgg tcaccccgtt taccctcggc
     5521 cctcccgcgt gcgaccctac cggatacagc tgcgtacagc gagcctttca ggacttctcg
     5581 ctcttcgccg acaatgcgca ctagagcctt tctcctcatc tcgttggcct tcctcggtgg
     5641 gtcgcggccc gccttagcag aatcggccga ggaggtcaca tatttcgtgc ccctcgttgg
     5701 tgtcctaagc cagataatcc ctgggcctca ggggccgacc tacgtctact ggacgagttc
     5761 caccggcgtg ttcaacaacg gcccgtcccc ggccatggtt catgtgaagg ccgcgtatgg
     5821 tggtggcctc tggatagcgt ccccccctcc ctgcttgaat taccagctcc gtgccggtgg
     5881 gggaagcagc ctggggggct gcgtgttcgg gcctccgggc gacggcggca acggattcat
     5941 cgagattgcc gtcgagcctt cgatcgtcgt gactgcggat atccagcgac tcctcaatgt
     6001 cccgaactgc gtcatcggtt cgattgacgg ctacggcctc atttctcagg gttccgcacc
     6061 cctaccggtg tatagaggct tcttctctgc tgaaagcact gtcgttgccg ggcccgtcga
     6121 ccttggctcc ttcgacggcc cgggtacgtg catcactgga ccggcgacgc accgacgtcg
     6181 ggtgaacgtc acgctcttca acggaggaac gtccgaggca acgtttacgg tgaccgccat
     6241 cccgctgcgt gcgagcagcg cgccgatcta cgagacccac gcggacgtcc cgcctcggga
     6301 cgtccggcag ctcaacgcga tcccgatccc ggtctcaagc gaccttccct acgaatttgg
     6361 ctatgacata cgcgtgtggc tgcgcatcac cgcgacccag cccttcctcg cctacgtttc
     6421 gacgatcttc gagggaggag agcccggcac caatgcggtc acagtgtacg aggcgaaaac
     6481 ctcgaattag ggtgcaatca tgaagaacgg tctcctccca cgcaggcttg gaatgaggcg
     6541 actcgcgctt gatgtagtgg ccacgattat cgcagtgaca gtcggctttg cggcgcccgc
     6601 ccaagtcgac cttgcgctcg gcaattaccc ttccaattcg agctgcgtca tcttgggtgc
     6661 gaaaatcgcg tttgagcttc ggattttttc ctcggggaat gccagcggag tcattcttag
     6721 ggacaagctc cccataggta cgagcttcgt cagcgcaacg cgcgcgccat tttcggtctg
     6781 caacgacgag gtgatgtggt cgatccccac cgtgtccagc gccggtgaca gcgtgacggt
     6841 cgtcgcccgt gtggatgcgg ttccgccgtc gagttacctc ttgaatctcg cttcagtatt
     6901 cggaaatgag tccgactacg ccgaagggaa taactcgttc gccagcccct tgattcgagt
     6961 cgtcacgacc ccggatgcgg ggtgcctctc agtcgtagcg ttgcagccgg gtgcgttcct
     7021 gtcttcgggc ggccagccca tgagcatcct cgggaatggc ttccaatcca acgcaacggt
     7081 gacgatcgcg ggttcccagc tcgggggagc gtcgtttatc tcgtccagcc agataaatgg
     7141 ggcttcccct gccttgccgc cagggcaata cgacctgacg cttacgaatc cgggaggggc
     7201 aaaggcaaca cttatcgggg gattcaacgt gtcggatccg atcccgctcg gcttcgtgtc
     7261 cgtgaccccc tgccgggtct tcgatacgcg tgacgcgtca ggctcgccct cgctcggcgg
     7321 gaacacctcc cgttccttcc ttgtagccgg gcgatgcggc gtgccaacag gggccaaggc
     7381 aatcgcgctc aacgttacga tcgtgccgac agggggcggc ggaagcctgg ctctttacgc
     7441 tggcgacatg gcgccgccgc tcgcgtcatt cctcagcttc gatcccggca gtgttcgcgc
     7501 taataacggg ttctatccac tcgcgacaaa cggttctgga accctcggga tcgttaacaa
     7561 ctctatcagc gcggttgatg tggttctgga cgcgttcggg tacttccagt agcccagcgt
     7621 cttcgactct acattccgag catcactctc gcgacgcgcg acatcatgtc ggcgttccag
     7681 ggcggatccc agacgagctc gaccttcgcg tccgtgacgc cgggaacggc cttcaccttc
     7741 gcctcgacct cgccgggcag ggagcccgcg acggggcagg ccggcgccgt gagcgtcatc
     7801 ttcacgtcca cgacgccctc gggcgacgcg gcgacggagt agatcagccc gagctcccag
     7861 atgttcacgg ggatctcggg gtcgaagcac ttcttgagcg cctcgtcgat gaccttctgc
     7921 tcgagaggag agcgggaggg agaagacgaa gattgcttag aaggcgcgtc agagggcacc
     7981 gcggactgcg cggccgtcgc cgcgtctgca gcgccctggc cgtcagccct attactttct
     8041 gagagaatct tctcttctcc catcttctct ttctctctat tcagtcttgg ccacttcatg
     8101 gacgttcttg agcgcgctct cgagcgtgtg ccacgcgagc gtcgcgcact tgacgcgcgc
     8161 cgggaactcg cggacgcccg agaagaccgc gagctttccg aggccggcgc ccgtggcctt
     8221 ctcgtcgggc gggcccgtga ggaggtcgtg gaactttccg tagagcgcct cggcctcctc
     8281 gagcgtcctg cccttcacgg cgtccgtcat gagggaggcc gacgccttcg agatcgcgca
     8341 gccggagccc tggaacgcca cgtcggcgat ccggtcgccg tccagccgga tgtagagctt
     8401 caggcggtcg ccgcagaggg ggttgtggcc cagcgccatg cggctcgggt ccgtcatctc
     8461 gcggtagttg tgcggcgact tgttgtggtc gaggatgact tcctggtaga ggtcgcggag
     8521 gtcggtcatc aggcgaagac ctcctgcacc ttcttcagcg cggccgcgag gacgtcgacc
     8581 tcctccacgg tgttgtagac cccgaaggag gcccgcgccg tcgcgccgat cccgtatcgg
     8641 tccatgacgg gctgcgcgca gtgctggccg gtccggatcg cgatgccctc gcggtccagg
     8701 atcgtcccga tgtcgtgcgg gtggacctcc ccgaggagga acgagacgac ggcggccttc
     8761 cgcggcgcgg tcccgacgat gcggaggccc tcgatcgccg taagcgcctc ggtcgcgcgg
     8821 gcgaggagcg cgtgctcgtg cgcggcgacg gcgggcaggc cgacgcccgt gagccagtcg
     8881 gccgcgacgc cgaacccgat gccgcccgcg atgttcgcgg tgccggcctc gaacttccac
     8941 ggcagctcgt tgtacgtggt cttttcgaac gtgacggacg agatcatgtc gccgccgccc
     9001 tggtacgggg gaagcttctc gagccacgcc tccgtgccgt agagcgcgcc gatgccggtc
     9061 gggccgtaca tcttgtggcc cgacagcgcg tagaagtcgc agccgagcgc cgcgacgtcg
     9121 atcgggaggt ggggcgcggc ctgcgcgccg tccacgagga cgggcacgtc cctctcgtgt
     9181 gccagccgga cgaactcttc cacggcattg atcgtgccga gcgcgttcga gacgtgggag
     9241 acggcggcga tccgcgtgcg gtcggagagg cgcttctcga actcctcgac gggaacctcg
     9301 cccgagtccg tgatcgggac gacgacgagc ttcgcgcccg tgcggccgca tgcgagctgc
     9361 cacggcacga tgtttgagtg gtgctcgagc cccgtgagga ggatctcgtc gccctccttc
     9421 aggaccgact gcgcgaagcc cgcggcgacg agattgatgg cctccgtcgt gccgcgcgtg
     9481 aagacgacct ccctcggggc gcgcgcgccg aggaaccggg cgaccttcgc gcgggcgccc
     9541 tcgtactcgg cggtcgcctt ctcggaaagg aagtgcgtcc cgcggtggat gttcgcgttc
     9601 gtctcctcgt agtacttccg gtctgcgtcg atcacggcac gcggcttctg cgtcgtgttc
     9661 gcgttgtcga ggtagacgag cttcctcccg tgcacggtct gccggaggat cgggaattgc
     9721 tcccggatcg cctcgacgtc gaatgcgggc gaaacgccgg gcaaggcggt ctcggggacg
     9781 acgttcatgc gttcaccgct gcggccgccg ccggcagcca ggcgtggagg cggtcctcga
     9841 ggagggcttt cacgtccggg gcggccacct tcgagacgac ctcgctgccg aaggcgtacg
     9901 tgaggagggc gcgcgccgcg ccctccgaga ggccgcgcga gcggaggtag aagagggcgt
     9961 ccgcgtcgat ccggcccgtc gtggagccgt gcgtgcaacg gacgtcgttg ttgtggatca
    10021 cgagctgggg cttgctgtcg acgagcgcgt cgttcgagag gatcaggttc cggttcgtct
    10081 ggcgggcgtc cgttttcacg gcgccctcgc gcacgatgac cttgccgtcg aacgcgcccc
    10141 ggccgttgcc gtcgaggatg cccttgtaga gctcgaggct gtcggcgcgc ggggccgcgt
    10201 ggtcgatgac ggtgtggcag tcgacgttct ggccggcggc cgcggcgaag aggccgtaga
    10261 gctcggttcc cgagccctcg ccggagagcg tcgcggcgat ctcgttgcgg accgtcgcgc
    10321 cgccgaagga gtagacgtga gaagccgagc gggcgccgcg cgcgtgcgag aggaagaggt
    10381 tcccgacgtg gaaggccagc cggccttcga gctggacctt gacgtgcgtg agccgcgcgt
    10441 tttccccgac gacggcctgc gtgaccgcgt tcgtgaagga catctcgtcc gtgccgagga
    10501 acacctcgac gacggtcgcg gccgcgttct cctcgaggac gacgaggttg cgcaggtgag
    10561 acatcagcgg cgccggcgtc ccgctcgcgg acgtcgagag gtggacgacg acgaggggag
    10621 cgggcgcggt gagccctttt ccgacgtgca cgagggcccc cgtgtcgagg aacgcgtcgt
    10681 tcagggcgac gaacgggtgc gcggacggag ccgcgacgag cgccttctcg acgcgctcgg
    10741 gggcgagacg gaggacggtc gcgaggttct cgaagaggat ctcgggcgga agcgtgtggg
    10801 aggtccgccg cacgagcctg ccgttcacga agacgaggcg ggggccgggc gaggagagac
    10861 cgagcgtccc gagccgggcc tcgagctcca cggcctgcgt gcgctcggcg atcgtgacgt
    10921 cgcgcggcgc gaggccgaag accgtccgcg cgagcgggag caggttcgtg tagcgccact
    10981 cctcgtcgcg cagggacggg aagccggcct tcacgaacga ggcgacgccg tcccggcgca
    11041 gcctcgtgag ccacggaggg ccggcgagtc cggacgcgaa ctccacggcc tgcgtcgcga
    11101 agacgtccac gccgtcgcgg atggtggagg gcctggcgga gggcgtcatg ctcagcgcgc
    11161 cgcggccgcg gcgtccgcct cgatccagcc gtagcccttc gcctcgagct cgagggcgag
    11221 gctcttgtct cccgacttga cgatccggcc gtccgagagg acgtggacga agtcggggac
    11281 gatgtagttc aggagccgct ggtagtgcgt gatgacgagc atcccgcgct tgccgtcgcg
    11341 gagcgcgttg acgccgccgg ccgcgatgcg cagcgcgtcg atgtcgaggc ccgagtccgt
    11401 ctcgtcgagg atcgcgaggc gcgggtccag gagcgccatc tggagcacct cgttgcgctt
    11461 cttctccccg ccggagaagc cctcgttgac ggagcggttc agcagcgcct cctccatctc
    11521 gaccagcttc gccttctcgt cgaggagggc gaggaagtcg atcgcgtcga gctcggcgag
    11581 cccgcgatgc cggcggacgg cgttgacggc cgtgcggagg aagtagttgt tcgagacacc
    11641 cgggatctcg acggggtact ggaaggcgag gaacacgccc tcgcgggccc gctcctcggg
    11701 cttcatcgcg aggaggtctt tgccgaggaa cgagaccgtt ccctgcgtga cggcgtaccc
    11761 cggccgaccg gacagaacgt gcgcgagcgt gctcttgccg gagccgttcg ggcccatgac
    11821 ggcgtgaacc tcgccgggga gaatcttcag gtcgatcccc cggagaatct cggtcccgtc
    11881 ctcttcgatc gaggcatgca ggtttcggat ttccaaaaga ggtgtgttga cgttgctcat
    11941 ctgctactcg cgcagcgggc gtgcgccgcc gccgctcttt cattcgaaga aatcttcatc
    12001 cgacagaacc ctcgagcgaa acgcccagga gcttctgcgc ttccaccgcg aactccatcg
    12061 ggagctcctt gaggacgcgc ttggcgaagc cgttgacgat gagggacacg gcgtcctccg
    12121 ccgagatccc gcgctgcatg cagtagaaga gctggtcctc gccgatcttc gacgtggacg
    12181 cctcgtgctc gagcgtggcc gagctgttct tgacctcgat gtacgggaac gtgtgcgcgc
    12241 cgcactggtc gccgatgagg agcgagtcgc actgcgagta gttgcgggct ccctcggcgg
    12301 acctgaggat cgtgacgccg ccgcggtacg tgttctgccc gtggccggcg gagatgccct
    12361 tcgagacgat cgtcgaccgg gtgttcttcc cgatgtggat catcttcgtg cccgtgtcgg
    12421 cctgctggta gttgttcgcg agggcgaccg agtagaactc gccgacggag tcgtcgccca
    12481 tgaggatgca gctcgggtac ttccacgtga tggccgagcc ggtctcgacc tgcgtccacg
    12541 agatcttcga gcgcttcccg aggcacttgc ctcgcttcgt cacgaagttg tagatgccgc
    12601 ccttgccctc gcggtcgccg gggtaccagt tctggaccgt cgagtacttg atcgtggcgt
    12661 cgtccagcgc gacgagctcg acgacggcgg cgtgcagctg gttcacgtcg cgcttcggcg
    12721 ccgtgcagcc ctcgaggtac gagaccgtcg cgccctcctc ggccacgatg agcgtgcgct
    12781 cgaactggcc cgtgtcggcc tggttgatgc ggaagtacgt cgagagctcc atggggcagc
    12841 ggacgccctt cgggatgtag cagaacgaac cgtccgagaa gacggcggag ttcagcgccg
    12901 cgaagaagtt gtcgcgcgac ggcacgacgg agccgagcca ctttcgcacg aggtccgggt
    12961 gcgtgtgcac ggcctcggag aacgagcaaa agatgatccc gacggaggcg agcttctcgc
    13021 ggaacgtcgt cgcgacggat acgctgtcga tgaccgcgtc cacggcgacg ccggccagga
    13081 tctcgcgctc cttgagcggg acgccgagct tctcgtacat cgcgagcagc tcggggtcga
    13141 cgtccgcgag gctcttcggg ccctccttcg cggacttcgg cgcggcgtag tagacgatct
    13201 tctggtagtc gatgggcggg tacgagacgc gcgcccaggt cggctcttcc atcgtgagcc
    13261 agtggcggta ggccttgagg cgccactcga ggagccactc gggctcgttc ttcttcttcg
    13321 agatgatccg gacgacgtct tcgctgaggc ccgccggaat cgactcggtt tcgacttccg
    13381 tcacgaagcc gtgctggtac tcgcgctgcg tcagctcgtt cagcagggcg tcgctcatgc
    13441 ggggctccgg tgcgcgggga gcgcggcggc cgaagcggcg gcgaagcggg gcgggagcgg
    13501 ccgcgtcatg tcggcgagcg tcaggccttc cagcgacgcg cggatcgcgt cgttgatgat
    13561 ctgccagttc ggtttcgtgg agcagaagct ctcgtgctcg cagcggacgg cgccgccgca
    13621 ctccgtgatc gaaacgggcc cctcgagagt cgcgatgatc gaggcgaccg tgatctcgcg
    13681 cggagggcgc gcgagggagt agccgccgcg cgttccgcgc tgcgaggcga ggaggccgcc
    13741 gtgggagagc tgcttgagga gcttgccgac ggtggggagg ggcagacgcg tgtcgtgcgc
    13801 gagctcgcgc gccgtgaacg cgggccggtc ctcgtgcgcc gcgaactgcg tcagcagcac
    13861 gatcccgtag tcggtcatct tggtcattcg gaacatcggg ttctccccgg ttcgatatcg
    13921 aaccggatcg gtactgtatg aaataggacg aattcagtca agggacgaag aacagggaat
    13981 aagagatctt cttagaatga gttagtgaga tcgaatctca gcgtcggctg gcagtccgca
    14041 gcgggctggc cgcctgggat tcatgccgcc cccgagccca attaccttct aagaaatccg
    14101 actcttttct ccggtcttcg gcggaaggac ctcgaacgcc ccgtagccga cgacgctcac
    14161 ggggtctccg gccgtgtcgc ccgaggtccg gaggtcgagg agcttgaccg gtagggacct
    14221 ccgccgggcc agctcgagca gcccgcggag cgggtagtag ccgcaggcgt cctcgcccga
    14281 gaggttgtcc ggctcgcgcg cgaggatcgc gtcggccgtc atccggtcga ggcgacgggc
    14341 ggtctcctgg tcgtggtagt ggctgaggtc cgtgctgacg acgacgaccg tgcccttttc
    14401 accgaggaac tccgcgatca ggtccgcggt cgcccgggag gagatctccg agaccgccac
    14461 cggcaggaac gtgaagcgat cgccgaggac gcgctgcagg aacgggagct gcacctccag
    14521 cgcgtgctcc ggggcgtgag gccggtcgtc cacggctgcg ccgcacgccg cggcccgcat
    14581 gcgaagcgcg gcatcgatcg gaacctcgcc gagaggcgtc gcccaggcgt cggccgcagg
    14641 gaccgccgca ccgtgaacgg gcacgcggtg cacggggccg aacgcgacga ctcgatcgat
    14701 catggcggcg tgcgggcgaa ggcgcgcgta cgcgctggcc gcgacgggtc cggagtagat
    14761 cgtcccggcg tgagggacga cgagtgcgag gggaagcggt tcctcggggg cggcgactgc
    14821 tcccgcgagg agggcgtcca cgtggcgagc gagcgcggcc ggggaggccg ggtagaacat
    14881 tccggccacg gccggtggac ggacccgggg tgcccccgtg cgatttgtct tcatgacgcg
    14941 gcgagatgga cgggaaggcg gcgggagccc caggaaccgg ggacttcttc gaaagcgccc
    15001 gggcagggcg ccccgcacga ggcacagacg ccgccctttg cgagggacca ttcgaagatc
    15061 tcgtatccgt ctcgtccgat gacccgttcg ccgcacctcg gacagtccgt gctctggccc
    15121 tgggggtcgt ggacgttgcc ggtgtagacg tagttcagcc cgttcttctt cgcgatctcc
    15181 cgcgccttct tcagcgtcgc ggcgggggtc gcggatgcgt cgcgcatctt ccagtccggg
    15241 tggaacgccg tgaagtggtg cgggacgtcc gggccgagat gctcgaggac ccagcgggtc
    15301 atggcgtcga tctcggattc ggagtcgttc atcccgggga tcaggagcgt cgtgatctcg
    15361 gtccagactt tcgtctcccg gacgaggtac tcgagcgtct cgaggacggg ctgaaggtgc
    15421 gcgccgcacg tcttcgcgta gaagtcttcc gtgaacgcct tgaggtccac gtttgcggcg
    15481 tccattgcgg caaagagctc cgcccgaggg ttcggctcga cgtagccggc cgtcacggcg
    15541 accgtcttca cgcccgcggc ccggcacgct gcggcgacgt cgaccgcgta ctccatgaag
    15601 acgacggggt cgttgtacgt gaaggcgacg ctccggcagc cgtattcgac ggcagccgcc
    15661 gcaatgcgcg ccggtgtcgc gtcggcggcg agggtgtcca tctcgcggga cttcgagatg
    15721 tcccagttct ggcagaactt gcaggcgaga ttgcagccgg cggtgccgaa cgacaggacg
    15781 gacgtgccgg gcaggaagtg gttgaggggc ttcttctcga tcgggtcgac gcagaagccg
    15841 ctggaccggc cgtacgtcgt caggacgatc tccccgccgc gcgccgcgcg gacgaagcag
    15901 tgtccttcct ggccgtcctt cagcctgcag aaacgcgggc agacgtcgca ctggacgcgg
    15961 tcgccctcgg cgtgccagta cttcgtcgga tggaaggcct cgggagccgt catgagccct
    16021 cgccctcgag gcgaatatgg gccgccgggg ggggggaggg caaggctcat ctcgggctac
    16081 tcgaagtagc cgttgacgtc gagaatcatg tggacggttc cggcggagtt gttctggacg
    16141 ccgatggtcc ccgatccgtc gctcgcaagc cggacgattg cgttgctggc
//
LOCUS       DAOMFY010000060        15848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1141_length_15848_cov_10.102767, whole genome shotgun
            sequence.
ACCESSION   DAOMFY010000060 DAOMFY010000000
VERSION     DAOMFY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15848
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_15848_cov_10.102767"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1670)
                     /locus_tag="PLB01_07040"
     CDS             complement(<1..>1670)
                     /locus_tag="PLB01_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQQ77095.1"
                     /translation="MHVYHYAPYEPTAFKRLMGRHATRARELDRMLRAELFVDLCAVV
                     KQGLRASVESYSIKKLEPFYDFTRKAELRAAGAARRAVEFGLQTGDLTVLTPEVKDLV
                     ALYNEEDCVSALRLRDWLETLRPAAPRPPLGGGTASEEAEKRGAEVQAAMDALLKGVP
                     DEKADRTPEQHACWLLAHMLEFHRREDKVTWWEFYRLRDLTDEDDLLDERKVIAGLKF
                     EKRTPPQGKRAKAPTDHYKCPSQDFEGGEGDELFTSDGKSLGTIDSIDRAARRIAIKL
                     RLNRIDDADHPRWAFAHSHVRPDALVASLMRLASDIAKNGFTPKSAARDLLLGNPPRL
                     KKGTFGPSNGEKPLDFATRTVLSLDRSLLAIQGPPGAGKTYTGARMIVACIKAGLKVG
                     ITAVSHKVIRNLLDAVLEAAREMKVFVSCVQKVKEKSETPTRGIEEVTDNADVLAALA
                     DGSAQVGAGTAWLWSREEYAGAVDVLFVDEAGQASLANVLAMAQAAKSVVLLGDPQQL
                     EQPQRGTHPEGVDASALEHILAGSKTIPEGRGIFLDKTWRLPPAICAFT"
     assembly_gap    1671..1770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1771..2081)
                     /locus_tag="PLB01_07045"
     CDS             complement(<1771..2081)
                     /locus_tag="PLB01_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77096.1"
                     /translation="MHLSSSGSLTLAATDLGRFLACRHLTGLDVEVALGKRKKPPVYP
                     DPFLELLINRGLAHEKEYTGKIEKAGSAVLDLTEFSGDDAVTRTVEAMKAGKPAIAQG
                     AL"
     gene            complement(2113..2355)
                     /locus_tag="PLB01_07050"
     CDS             complement(2113..2355)
                     /locus_tag="PLB01_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQQ77097.1"
                     /translation="MKLPRDLSGLDLAKALSGVGYRVTRQTGSHLRLTNDGPPQHHVT
                     IPAHDPLKLGTLSAVLAEVASNLKTTREELATRLFS"
     gene            complement(2352..2558)
                     /locus_tag="PLB01_07055"
     CDS             complement(2352..2558)
                     /locus_tag="PLB01_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HQQ77098.1"
                     /translation="MSEVIFLVEDAPEGGYIARAVSAAIFTQAKDLDELQERVRDAVR
                     CHFDEGSAPKLIRLHFVKDVVIAA"
     gene            complement(2619..3599)
                     /locus_tag="PLB01_07060"
     CDS             complement(2619..3599)
                     /locus_tag="PLB01_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQQ77099.1"
                     /translation="MPFNDRPYFRRLLDFDDLVRRGRPVTCESLAERWETSTKTVQRF
                     VDQIRTDFEAPVVFDRKKHSFVYEDPDWRFPFLDVSGADLFAIGVAMKVFQIYEGTPV
                     LKDLKATFERIARLLPESVRIQPSSMIEKLWVHPQPVRAIAKEVWAATANALREKTQL
                     EIDYHKPTGDGSRRLVEPLVLVHMNWDWFLAARDPEDGKTKTFYLARIKEAKNTNFHF
                     IPPKDFDAARHFGDSGIGLYVGGKPFRFKVKIAKEIAPWITEVRWHPAQKLETLKNGD
                     VVLDLPAGSLIEAKRFVLSLGRFGKALGPKELVGEMRTEIAQMRKDYGAA"
     gene            complement(3765..4319)
                     /locus_tag="PLB01_07065"
     CDS             complement(3765..4319)
                     /locus_tag="PLB01_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HQQ77100.1"
                     /translation="MRNLVLACAALILVCTSVRAESGPPDGEPASPRVEGKDANRVRT
                     AMENIRQIAIAIESFAVDNNRYPRPDRTTDSVGPYRLGPVASIQRALASGGKDLPVVD
                     PWGNPYLYWSSGQDYVLVCQGSDGTLTDRALVQMVIHWEAGSEGHGVDNLGPLPPLKC
                     SAQEIIFGDGLFLRYPKGPLGDCR"
     gene            4517..4747
                     /locus_tag="PLB01_07070"
     CDS             4517..4747
                     /locus_tag="PLB01_07070"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HQQ77101.1"
                     /translation="MKPPLNTLVAIPNVSAPCPVNTCLATLPDGTTTCYPCLEAPPLG
                     PAADVPTLDTWALLLLGVALVAAGFLESRSRG"
     gene            4744..5859
                     /locus_tag="PLB01_07075"
     CDS             4744..5859
                     /locus_tag="PLB01_07075"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HQQ77102.1"
                     /translation="MTLVAARGPSRSDRIAGCLLGGAVGDALGAPIEFLRLSEIRERF
                     GPEGLTDYAPAYGKLGAITDDTQMTLFTAEGLLRAMCRAATKGICDGPTVVYFAYLRW
                     LKTQGYDSKSESFPFATEDLPRLDPEGTYPNGWLSTVRALRSRRGPGSTCLAALRSGR
                     FGRVEDREPINSSKGCGGVMRAAPAGVVADSATRAFELGCQTAAITHGHPTGWLAAGA
                     LAAIVYELLRGLTPRESVDVALRLLKDEQRSGECRDALAAAAELTRRGAPSVGKLATL
                     GEGWLAEEALAIGVYAALSAETFRDGVLLAVNHGGDSDSTGSIAGNILGAALGREAIP
                     EPWLERLELRDVIETISADLVTGFEETDEWWRKYPGC"
     gene            complement(5902..6213)
                     /gene="nadS"
                     /locus_tag="PLB01_07080"
     CDS             complement(5902..6213)
                     /gene="nadS"
                     /locus_tag="PLB01_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HQQ77103.1"
                     /translation="MKKELFDELLASVREGGAILRGSRRAARTMKFAEPDVRLLRERY
                     GLTQSKFAALMGISVATLRNWEQGRRRPEGSAKVLLRVVAKHPEVVLESAAGGAGTGR
                     S"
     gene            complement(6728..6943)
                     /locus_tag="PLB01_07085"
     CDS             complement(6728..6943)
                     /locus_tag="PLB01_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQQ77104.1"
                     /translation="MKSPADTAKVMEAAGIKPGDTVVSYCHIGQQATVMYVAARRLGY
                     KALLYDGSWDEWSRKPELPVEKSAPKP"
     gene            complement(7431..7913)
                     /locus_tag="PLB01_07090"
     CDS             complement(7431..7913)
                     /locus_tag="PLB01_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77105.1"
                     /translation="MLNPVEKFVVGAYWGSRAEMPEGCAQRLNQLLDGLKSLDPVFAE
                     WFAKGPSAGTSRTRILSPADIFALAVRGMNRRDLDASPIPSLGVRIGVWNGGREGTTA
                     VGISTGCGSLDEPGGVPALNSLLISLPNQGPDADRILDVKALEKMLEVVVRAYDLTGR
                     "
     gene            complement(7970..8317)
                     /locus_tag="PLB01_07095"
     CDS             complement(7970..8317)
                     /locus_tag="PLB01_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77106.1"
                     /translation="MSDLAAAVLDRLGYLVVYAPDIPSTHELTLVRQFAQIQEDIIAL
                     ITKQRREETRQQLEVAMNELKDAERAFNENLRDKGRQLIFDAERRVQQSLGARPPRTT
                     FVSGPSGTKNVRG"
     gene            complement(8314..10218)
                     /locus_tag="PLB01_07100"
     CDS             complement(8314..10218)
                     /locus_tag="PLB01_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQQ77107.1"
                     /translation="MKRSLVSALASLLLAMDVRAAEPRWTVEFKEGAYTFLTATGLEV
                     GQKTGPGAIAGYLVVKLPPDVDPATTNRGVFLGRVRVPERRVTWLKGDEFAAGELLPA
                     HHLIAITPNAGFEPGLWAVKVRDTVKDRSGRPVNPTNATWTVEIGEPRARPDDPGPAR
                     NTLKLKFTTEVDPATYADGIVVEKQVAGNWQEEQRGSATIAPSAHGGSVNEIGLRPLG
                     GWERGATYRVRVTDAIRDVRGQPLTNAEDFSFTVPKSGPFETRHEPRRPVAQASAQAA
                     AAGPAFPGGQALGWHGHWTDPVTGLIYMRHRWYDPRTGTFLSPDPLEDIDSPNQYLFG
                     AGRPHEVVDPLGLCLGLDKEGRPCGYYADKIDEYLVETAKLRRDQTGVPLAIDRLLLS
                     PVTGFLRLGEAQGRLGYRYAEALTNPRVALPAGATVTSETNEVGADLLQAAGDAALLA
                     PITRSGAAGAKVSLGMPEGSALASAVRVPLTLQAPKALPALPPASVESAIASSYQQFI
                     NEAAQAVVVSFNRGEIPVPAGVSWQTILGQRIDAAARTRLRDLVRSLGIPEGPGTDVL
                     INRRLTDPAGSGRFRIPDLRLVKSRRILDGTIGAKDSSTSQVKDFEMFSLGDTVEIVR
                     PQVGPLQVPR"
     gene            10387..10719
                     /locus_tag="PLB01_07105"
     CDS             10387..10719
                     /locus_tag="PLB01_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQQ77108.1"
                     /translation="MKPPEKPLAWMHGGIKSPPFSKEGRVRAGYLLRGLQQGNVLSLP
                     DSRPMPEIGKGCHELRVPDGNVDWRIFYAVRPDAIVILGVEKKGTQHTPKATIAACAR
                     RLAAWDRE"
     gene            10724..11035
                     /locus_tag="PLB01_07110"
     CDS             10724..11035
                     /locus_tag="PLB01_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ77109.1"
                     /translation="MDKAKKARLTKKGWTFGSAETFLGLTPEESAYIEIKLSLARELK
                     KARLKKGLSQTALASKLGSSQSRVAKMEAADEQVSLDLIVRSLLATGKRPREIARAVA
                     K"
     gene            complement(11131..12027)
                     /locus_tag="PLB01_07115"
     CDS             complement(11131..12027)
                     /locus_tag="PLB01_07115"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HQQ77110.1"
                     /translation="MKNALAALLFLASAAAPAADILVTPEWLAAHQRDKNLVLLHVGV
                     PAEFEKEHIPGAIPVTPADFAFPRADGALILQLLPPDQLKAKLEGYGISDDSRVVVYF
                     AKDWVSPTTRVYFSLDAAGLGERTSILDGGMPAWKAAGGPAVASANDPASAVQRKPGK
                     ITAKPHPELVADLDLVKASIRKSGVAIVDSRNPKFYDGEDAGNMPRAGHIPGAKTLPF
                     DTLVNDDNTMKSPADTAKVMEAAGIKPGDTVVSYCHIGQQATVMYVAARRLGYKALLY
                     DGSWDEWSRKSELPVEKSAPKK"
     gene            12132..13307
                     /locus_tag="PLB01_07120"
     CDS             12132..13307
                     /locus_tag="PLB01_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQQ77111.1"
                     /translation="MSFADLWTLDPAVDYLNHGSFGACPKAILEKQSELRARLEREPM
                     DFLVRHAPGLYADARTALGQFVGADPDDLAFVPNATTGVNAVVRSLAFSPGDEILTTD
                     HAYGACRKTLEYVAARAGARVTIAKVPFPVAGPDDVVSAVLSAVTPRTRLAMLDHVTS
                     PTALVFPVERLVAELSARGVDTVVDGAHALGMLPLDLTRLGAAYYTGNAHKWLCSPKS
                     VAFLYVRKDRQKLVHPVTISHGYAGGEARFRDEFDWTGTIDPTPALTLPFCIEYLGGL
                     LPGGWPALMEKNRDLALRARGILLERLGGTPACPSAMVGSMAAVILPPLPPGSPAARL
                     DRERLADWMRARGSEPWFFGWPPAGGGKLVRVSAQLYNAEEQYVRLAGLLAEALREG"
     gene            13297..14610
                     /locus_tag="PLB01_07125"
     CDS             13297..14610
                     /locus_tag="PLB01_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQQ77112.1"
                     /translation="MKAEGIEARLGTFDTAMVVVSLVVGIGIFRTPAIVAKAAGTEGR
                     FLLAWALAGLVSLVGALVFAEIGSRHPRAGGYYRVVADCYHPALAFMLNWAQTLMQGA
                     GAAGVAFIGAEYLVGLLLPPEKRGGSAVLLVALALMLVLLVLNGLGIRVGAGTQNVLS
                     MTKIVMIAGLAGVAFVFAPRFAGASAAPSAAPAGLAAALIPCFYAYGGYHMTMNLGAD
                     VKDARRRFPLAVTFGMLVVAGLYLVLNWAYVRTLGAAGVAGSNLVAAALARASLGAGG
                     EAVVSALIFLSAAGFVNATVLQMPRSYHAMAEDGVLPAVFRRVDPRTQVSLAGLALFG
                     ATMLVPAFLLGSFEKLLNYIIFTDMLTLAVVASTLFVLRRRGEGDGAFRIPGFPVLPA
                     LYVLALAAVAAHVAVTEPALALAGTLILLAGGPLFVLGRKLNRAG"
     gene            14659..15048
                     /gene="cutA"
                     /locus_tag="PLB01_07130"
     CDS             14659..15048
                     /gene="cutA"
                     /locus_tag="PLB01_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HQQ77113.1"
                     /translation="MAKPASPPALIAVLTSVGTEQQALDIAHALVKRRLAACVNILPG
                     VRSLFRWKGKVQQDAELLLVVKTLASNFEAVRGVIKELNAYELPEIVAFPAAMADSAF
                     AAWVHDSSTGDASDDDEEDEPGEATID"
     gene            15135..>15848
                     /locus_tag="PLB01_07135"
     CDS             15135..>15848
                     /locus_tag="PLB01_07135"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HQQ77114.1"
                     /translation="MRRLLPCLILAAALPGAPLAANSVTVQPVADTSIYEENGDQSDA
                     KGPGIFAGRNVLTAIRRAFVRFDVAAAVPAGSTIDGVRLDLVLLSGKGNPVDVNLFRV
                     TAAWGEGTSDAGSPGGTGAPATPGDATWTRRIWPGTAWVTPGGDTVGFFSARIPVQTT
                     LTTYSFGPTAAMQADVQSWLDAPATNFGWQVRADELQAAPSARRFGSPESANAAQRPP
                     LTIPFTPPGGGPGSRPADDV"
BASE COUNT         2348 a         5185 c         5509 g         2706 t
ORIGIN      
        1 gtgaaggcgc agatggcggg cgggaggcgc cacgtcttgt cgaggaagat gccgcggccc
       61 tcggggatgg ttttgctgcc ggcgaggatg tgctcgaggg ccgaggcgtc gacgccttcg
      121 gggtgcgtgc cgcgctgcgg ctgctcgagc tgctgcgggt cgccgaggag gacgacgctt
      181 ttcgcggcct gggccatggc gaggacgttg gcgagggagg cctggccggc ctcgtccacg
      241 aagaggacgt cgacggcgcc ggcgtactcc tcgcgcgacc agagccaggc cgtgccggcg
      301 ccgacctgag cggagccgtc ggcgagggcg gcgaggacgt cggcgttgtc ggtgacttct
      361 tcgatgccgc gggtcggcgt ctccgacttc tccttcacct tctggacgca ggagacgaag
      421 accttcattt cgcgggcggc ctcgaggacg gcgtcgagga ggttgcggat gaccttgtgg
      481 ctgacggcgg tgatgccgac cttgaggccc gctttgatgc aggcgacgat catccgcgcg
      541 ccggtgtacg tcttccccgc tccgggcggg ccctggatgg cgagaagcga gcggtcgagg
      601 gagaggacgg tgcgcgtcgc gaaatcgagc ggcttctcgc cgttcgatgg cccgaatgtg
      661 cccttcttga ggcgcggcgg gttgccgagg aggaggtcgc gggcggcgga tttcggggtg
      721 aagccgttct tggcgatgtc ggaggcgagg cgcatgagcg aggcgacgag tgcgtcgggt
      781 cttacgtgcg agtgcgcgaa cgcccagcgc gggtggtccg cgtcgtcgat gcggttgagg
      841 cgcagcttga tggcgatgcg acgggcagcg cggtcgatgg agtcgatggt gccgagggac
      901 ttgccgtcgc tggtgaacag ctcgtcgcct tcgccgccct cgaagtcttg cgacggacac
      961 ttgtaatggt cggtgggcgc cttggccctc ttgccctgcg gtggcgtcct cttctcgaac
     1021 ttcaggcccg cgatcacctt gcgctcgtcg aggaggtcgt cttcgtcggt gaggtcgcgc
     1081 agccggtaga actcccacca cgtgaccttg tcctcgcggc ggtggaactc gagcatgtgg
     1141 gcgagaagcc agcaggcgtg ctgctcgggc gtgcggtcgg ctttctcgtc gggcacgccc
     1201 ttcaggagcg cgtccatggc ggcctgcact tcggcgccgc gcttctcggc ttcttccgag
     1261 gcggtgccgc cgccgagcgg cgggcgcggg gcggcgggcc gcagggtttc gagccagtcg
     1321 cgcaggcgga gggccgagac gcagtcttcc tcgttgtaga gggcgacgag gtctttgact
     1381 tcgggggtga ggacggtgag atcgcccgtc tggagcccga attcgacggc ccggcgggcg
     1441 gcgcccgcgg cgcgcagttc ggccttgcgc gtgaagtcgt agaacggctc gagcttcttg
     1501 atggagtacg actcgacgct ggccctcagg ccctgcttga cgacggcgca gaggtcgacg
     1561 aacagctcgg cgcgcagcat gcggtcgagc tcgcgggcgc gcgtggcgtg ccggcccatg
     1621 agcctcttga acgcggtggg ctcgtagggc gcgtagtggt agacgtgcat nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcgcgccct gcgcgatggc gggcttgccc
     1801 gccttcatgg cttcgacggt gcgcgtaacg gcgtcgtcgc cactgaactc ggtcaggtcg
     1861 aggacggcag agcccgcctt ctcgattttc cccgtgtatt ccttctcgtg agcgagtccg
     1921 cggttgatga ggagctcgag gaaggggtcg gggtaaaccg gcggtttctt gcgcttgccc
     1981 agcgcaacct cgacgtcgag gccggtgaga tgccggcacg cgaggaagcg gccgagatcg
     2041 gtggcggcga gggtgaggga gccggaggag gagaggtgca tcagtagcac ctcacggcaa
     2101 tgcccgaggt cgctatgaaa agaggcgcgt ggcgagttct tcgcgggtgg ttttcaggtt
     2161 tgaggccacc tccgcgagca cggcggaaag ggttccgagc ttcagggggt cgtgtgcggg
     2221 aatggtcaca tggtgctgcg gcggaccgtc gttcgtcagc ctgaggtggc ttccggtctg
     2281 acgggtaacc cggtaaccca cgcccgagag agccttggcc agatcgagac ccgacaggtc
     2341 ccgcggcaac ttcatgcggc gatgacgacg tctttgacga agtgcaaccg gatgagcttc
     2401 ggagcggagc cctcgtcgaa gtggcagcga acggcgtcgc gcacgcgctc ctggagctca
     2461 tcaaggtctt tcgcctgcgt gaagatggcc gcactcaccg ctctggcgat gtagccgccc
     2521 tccggcgcgt cttccacgag gaagatcact tcgctcatga ccccgaatcg taggccgtat
     2581 cgcccgcacg gtcaaaggac ccgcttcgaa aagagaggtc atgccgcccc gtagtccttc
     2641 ctcatctgcg cgatttccgt cctcatctcc cccaccagct ccttcggccc cagcgccttc
     2701 ccgaatctcc ccagcgaaag cacgaagcgc ttggcttcga tgagggagcc ggctgggagg
     2761 tcgaggacga cgtcgccgtt cttcagggtc tcgagcttct gcgcggggtg ccagcgcacc
     2821 tcggtgatcc agggggcgat ctccttggcg attttgacct tgaagcggaa gggcttgccg
     2881 ccgacgtaga ggccgatgcc cgagtcgccg aagtgcctgg cggcgtcgaa gtccttcggc
     2941 gggatgaagt ggaagttcgt gttcttcgcc tctttgatgc gggcgaggta gaaggtcttc
     3001 gtcttgccgt cctcggggtc tcgcgcggcg aggaaccagt cccagttcat gtggacgagg
     3061 acgagcggct cgacgaggcg gcgtgagccg tcgcccgtgg gcttgtgata gtcgatctcg
     3121 agctgcgtct tctcgcgcag ggcgttcgcc gtggcggccc acacctcctt cgcgatggcg
     3181 cgcacgggct gcgggtgcac ccagagcttc tcgatcatcg acgacggctg gatgcgcacc
     3241 gattccggca acaggcgcgc gatgcgctcg aacgtggcct tgaggtcctt gaggacgggc
     3301 gtgccctcgt agatctggaa gaccttcatc gcgacgccga tggcgaagag gtcggcgccg
     3361 gagacgtcga ggaacgggaa gcgccagtcg gggtcctcgt agacgaacga gtgcttcttg
     3421 cggtcgaaga cgacgggggc ctcgaagtcc gtgcggatct ggtcgacgaa gcgctggacg
     3481 gttttcgtgg acgtctccca gcgttcggcg agggattcgc acgtgacggg tctcccccga
     3541 cgcacgagat cgtcgaagtc caggagccta cggaagtagg gcctgtcgtt gaaaggcatg
     3601 aatccatcgg acggtagcaa ttcctccgcc agcgcgtaat gcgtcatgtg catcaggtaa
     3661 acgctcgaaa cggacagggg atgtccgcga aatgtcggga aagcctcgag tgcatcggac
     3721 gaaaagtggc gttacgcgcc ctggacgggt ccgcgcagcc gccgtcagcg gcagtcgccg
     3781 agcgggccct tcgggtatcg aaggaagaga ccgtcgccga agatgatttc ctgcgccgag
     3841 catttgagcg gaggcagcgg tccgaggttg tcgacgccgt gcccttcgct gccagcctcc
     3901 cagtggatca ccatctggac gagagctctg tcggtcagcg tcccgtccga accctggcag
     3961 acgagcacgt agtcctggcc ggaagaccag tagaggtacg ggttgcccca gggatcgacg
     4021 acgggaaggt ccttcccgcc cgacgccagc gcacgctgga tcgacgcgac cgggccgagc
     4081 cgataggggc ccaccgagtc cgtcgtccgg tcgggacgcg ggtatcggtt gttgtcgacg
     4141 gcgaatgact cgatcgcgat cgcgatctgc cggatgtttt ccatcgcggt gcggacccga
     4201 ttcgcgtcct tcccctcgac ccgggggctg gccggctcgc cgtcgggtgg cccggactcc
     4261 gcccggacgc tcgtgcaaac gaggatcaac gcggcacagg ccaggacgag attgcgcatc
     4321 ggatgccctc cggcgctcat gttgtggacg gggcccggac aggcgatgtc cgggaaaacg
     4381 gctcacgcgc aaggaactcc ccctacccct gtagacagtt cagaagcgcc gttttcaacc
     4441 ggggctgggg tgatgcacct gaatgggtcc acacggactg ttcatgtcct tgtcgcgcga
     4501 acaggttggc gttgaggtga agccgccctt gaacacgctc gtcgcaattc cgaacgtatc
     4561 cgcgccgtgc cccgtcaaca cgtgcctcgc gaccctgccc gacggcacga cgacctgtta
     4621 cccgtgcctc gaggccccgc ccctggggcc agcggccgac gtgccgacgc tcgacacgtg
     4681 ggcgctgctg ctgctgggcg tcgcgctggt ggcggcggga tttctcgagt cgaggagcag
     4741 ggggtgacgc tcgtggccgc gcgcgggccg agccgctccg atcgcattgc cggatgcctg
     4801 ctcggcggcg cggtgggcga tgcgctgggg gcgccgatcg agttcctgcg gctgtcggag
     4861 atccgcgagc gcttcgggcc cgaggggctg acggattacg cgccggcata cggaaagctt
     4921 ggggcgatca ccgacgacac gcagatgacg ctgttcaccg ccgaggggct gctgcgcgcg
     4981 atgtgccggg ccgcgacgaa gggcatctgc gacgggccga ccgtcgtcta cttcgcgtac
     5041 ctgcgctggt tgaagacgca gggttacgac tcgaagagcg agagcttccc cttcgcgacc
     5101 gaagacctgc cgcggctgga tccggaggga acgtatccga acggctggct ctcgaccgtg
     5161 cgggcgctgc gctcgcggcg cgggccgggc tcgacgtgcc ttgccgcgct ccggtccggg
     5221 aggttcgggc gcgtggagga tcgcgagccc atcaactcgt cgaagggctg cggcggcgtg
     5281 atgcgcgcgg cgcccgcggg tgtggtggcc gactcggcga cgagggcgtt cgagctgggc
     5341 tgccagacgg cggccatcac gcacggccac cccaccggct ggctggccgc cggggcgctg
     5401 gccgcgatcg tctacgagct gctgcgcggg ttgacgccgc gcgagagcgt ggacgtcgcc
     5461 ttgcggctcc tcaaggacga gcagcgctcg ggcgagtgcc gcgacgcgct ggccgcggca
     5521 gccgagctga cccggcgcgg cgcgccgtcg gtcggaaagc tcgcgacgct cggcgagggc
     5581 tggctggccg aggaggcgct cgcgatcggc gtttacgcgg cgctttcggc cgagaccttc
     5641 cgcgacgggg tgcttctcgc cgtgaaccac ggcggcgaca gcgacagcac gggctcgatc
     5701 gccggcaaca tcctcggagc ggcgctgggc cgcgaggcga ttccggagcc gtggctcgag
     5761 cggctcgagc tgagggatgt catcgagacg atttcggcgg atctcgtgac gggattcgag
     5821 gagacggacg aatggtggcg aaagtacccg gggtgctgaa gaagcgcgcg aagccctgcc
     5881 agaagtgcgg taacaggcgg ctcaagaacg tccggttcca gcgcctcccg ctgcgctctc
     5941 gaggacgact tcgggatgct tcgccacgac acgaaggagc accttggcgc tgccttccgg
     6001 ccggcgccgg ccctgctccc agttcctcag tgtggcgacg ctgatgccca tgagggcagc
     6061 gaacttcgac tgcgtgagcc cataccgctc tctgagcagg cggacatcag gctccgcgaa
     6121 cttcatcgtg cgagcagcgc ggcgcgatcc gcgcaggatg gcgccgcctt cacgcacgct
     6181 ggccaggagc tcgtcgaaca attccttctt catggatact ccgtttcgat cacacgcttg
     6241 agcgtccgtt tctgtgtctc cgacaggtcg tcgcgttcgt tcttggcgta tgccagcgag
     6301 catggcctga aggcgccggt agtcttcatc cttcaggagc atccgaatcc gggctgtgaa
     6361 tggccccgtt tccacgatga ccacccgcag atgatacgcc attggcgtat gccgcgggat
     6421 actccttcgc ggacagcccc tgtccgtgtc tcccctccag attgatggcg agaagggaga
     6481 tccatgagcg acaggaagtc acgatcgaag ccctgcccga agtgcggcag cctcgacgtc
     6541 gttcccatcg agtacggcta cccggggccg gagatgatgg acgcggcgga gaaggggctg
     6601 atcgagctgg gcggctgctg cgtcaatggg ggacgacccg cggaagcact gcacggcgtg
     6661 cggcgaagag ttcgaccggc cgccggcgcg cgcgagccga agcggcggct gaccgcgcgc
     6721 cgcggaacta cggcttcggc gcgctcttct ccaccggcag ctcgggcttg cggctccact
     6781 cgtcccacga gccgtcgtag aggagcgcct tgtagccgag gcgccgggcg gcgacgtaca
     6841 tgacggtggc ctgctggccg atgtggcagt aggagacgac ggtgtcgccg ggcttgatgc
     6901 ccgcggcctc catcaccttc gccgtgtcgg ccgggctctt catggtgttg tcgtcgttga
     6961 cgagggtgtc gaacggcgga gtctggacgc gggtccccga acacctcggt cgatggagtc
     7021 catgacgagc gcgctagaag ttgcgtagag gccgtgtccc tcagccttct tcaaaatcgc
     7081 atctgctcgt tccacggcag gtgttcgcgg cacatggcca gcgttgaatc gtcggtggtc
     7141 gaagacgaat gccttgtggt cgagcgacat cgcgtccacc tacggtcttc ggctgatagc
     7201 ttgttccagc gctcgcgcct gcgcgatgtg aacagggttg ccgggagaga atctctcttc
     7261 ggtcgtaacc caggccgtgc cgaaggcagt gacaccgagg cgtttcaggg ccaacacgtc
     7321 cgccgccacc gtcgcctttc tgtttcgcct ggacagatac gtgatccatc ccgcgtgtgg
     7381 cccgcgctca ggaccagagg gccacagctt catgtgctct cgtgatgcaa ctaccgccca
     7441 gtcaggtcgt aggcgcgaac gaccacttcg agcatctttt caagtgcctt gacgtcgagg
     7501 atgcggtcgg cgtccgggcc ttgattcggt aggctgatga gaagcgagtt caaggccggc
     7561 acgccgcctg gctcatcgag ggagccacag cccgtgctga ttcccaccgc ggtcgttccc
     7621 tcgcggccgc cgttccaaac tccgattcgc accccaagcg aagggatagg cgaggcgtca
     7681 aggtcgcgcc gattcattcc acgcacagcg agggcaaaga tgtccgcggg cgagagaatc
     7741 ctcgtgcggc ttgttcccgc tgacggccct tttgcaaacc attcagcgaa caccgggtcc
     7801 agggacttca gcccgtcgag cagttgattc agcctttggg cgcagccctc cggcatttcc
     7861 gcccgactcc cccagtaggc gccgacaacg aacttttcca ctggattcag catgaaagcc
     7921 ttgtcggagg ttgttctctc caacgcactc ggcgcgcacc ccgacggcct tagcctctta
     7981 cgttcttcgt accggatggc cccgagacaa acgtcgtcct cgggggccgc gccccgaggc
     8041 tctgttgcac gcggcgctca gcgtcgaaaa tgagctgacg ccctttgtca cgaagattct
     8101 cgttgaaagc ccgctccgca tccttgagtt cgttcatcgc cacttctagc tgttggcggg
     8161 tctcttccct tcgctgttta gtgattaggg caatgatgtc ctcttgaatc tgcgcgaact
     8221 gacgtactag ggtcagttcg tgagtcgatg gaatgtcggg ggcgtagacg acgagatacc
     8281 ccaagcgatc caagacggcc gcagcgaggt cactcatcga ggaacttgca gtggcccgac
     8341 ttgaggccgc acgatctcga ctgtatcgcc cagagagaac atctcaaagt ccttcacctg
     8401 gctcgtcgag ctgtccttcg ctccgatcgt gccatccaga atccttcgac tcttcacgag
     8461 ccttagatct ggaatcctga atctcccgga gccggctggg tctgtcaatc tcctattaat
     8521 caatacgtca gtgcctggcc cctccgggat tcccaggctt cgtacgaggt cacgaagacg
     8581 tgtgcgagcg gccgcgtcaa tccgttgccc aagaatcgtc tgccaggaga ctcccgcggg
     8641 aaccggaatt tcaccccgat tgaaggatac gaccactgcc tgcgcggctt cgttgatgaa
     8701 ctgctggtaa ctggaggcaa ttgcactctc tacactggct ggagggaggg ctggcagagc
     8761 cttcggcgcc tgaagggtga gcggaacacg aactgcagag gcgagggcag acccttcggg
     8821 cattcctagc gagacttttg ctccagccgc tccgctcctc gttatcggcg ccagcagcgc
     8881 tgcatcgccg gcagcctgca gcaggtcggc tcccacctcg ttcgtctcgc tcgtgacggt
     8941 cgcgcctgcc ggaagagcaa ccctgggatt ggtcaaggcc tcggcgtatc ggtagccaag
     9001 ccgaccctgg gcctcgccga gtcgtaggaa tccggtaacc gggcttagca acagacggtc
     9061 gatcgccagc ggaacgcccg tctgatctct tcgcagcttc gccgtctcaa ccaggtattc
     9121 gtcaatcttg tcggcgtagt agccgcatgg gcgaccttcc ttatcgagcc caaggcacag
     9181 ccccaacgga tccaccacct catgcggcct acccgctccg aacaagtact ggttcgggga
     9241 gtcgatgtcc tccagcgggt ccggcgacag gaacgtcccc gtccgggggt cgtaccagcg
     9301 gtggcgcatg tagatgaggc ccgtgacggg gtccgtccag tgcccgtgcc aacccagcgc
     9361 ctgcccgccc gggaacgccg gccctgccgc cgctgcttga gcgcttgcct gcgccaccgg
     9421 ccgccgcggc tcgtggcgtg tctcgaacgg cccggacttc ggcaccgtga aactgaagtc
     9481 ttcggcattc gtgagcggtt gcccgcgcac gtcgcggatc gcgtccgtga cgcgtacgcg
     9541 gtacgtcgcg ccgcgctccc atccgcccag tggtcgcagt ccgatctcgt tgacagagcc
     9601 gccgtgcgcg ctcggcgcga tcgtcgcgct gccccgctgc tcttcctgcc agttgcccgc
     9661 gacctgtttc tccacgacga tcccgtcggc gtacgtcgcc gggtcgacct cggtcgtgaa
     9721 cttcagcttg agcgtgttgc gcgccgggcc gggatcgtcc ggccgcgcgc gcggctcccc
     9781 gatctcgacc gtccacgtcg cattcgtggg gtttaccggg cgcccgctcc ggtccttgac
     9841 ggtgtcgcgg actttcaccg cccacagccc gggctcgaag ccggcgttcg gggtgattgc
     9901 gatgaggtgg tgcgcgggca gcagctcgcc ggccgcgaac tcgtcccctt tcagccacgt
     9961 gacgcgtcgc tcgggcacac gcacgcggcc gagaaagacg ccgcggttcg tcgtggccgg
    10021 atctacgtcg ggaggaagct tgaccacgag gtatcccgcg atggccccgg gacctgtctt
    10081 ctgtccgacc tccaggcccg tcgccgtgag gaaggtgtac gcgccttcct tgaactcgac
    10141 cgtccagcgg ggctcggccg cccggacatc catcgcgagg agaagcgaag ccaaggccga
    10201 tacgagcgac cgcttcatcg atgtaccccc ggtggcgggg aaaatcctat cgctcgagat
    10261 caggggcaag tccagccccg ggtcgcaagg accccgatcc caccttccga tatgcctccg
    10321 agaggagccg tacttatgtc aatattgaca tagtcgatca atcgccctac tctcgtgccg
    10381 gatgaaatga agccccctga gaaacccctc gcgtggatgc acggcggaat caagtctcca
    10441 cccttttcga aggagggccg ggttcgcgcg ggatatctct tgcggggcct tcagcaaggg
    10501 aacgttctct cgcttcctga ttcacgaccg atgccagaga tcgggaaggg ttgccacgag
    10561 ctgcgagtgc ccgacggcaa cgtcgactgg cggatcttct atgcggtccg gccggatgcg
    10621 atcgtgatcc tgggagtcga aaagaagggt acgcaacaca cgccgaaggc cacgatcgca
    10681 gcgtgcgcgc ggcgcctcgc ggcatgggac agggagtaat cagatggaca aagcgaaaaa
    10741 ggctcgcctc acgaagaagg gctggacgtt cggatccgcc gagaccttcc tcggcctgac
    10801 tccggaggag tcggcctaca tcgagatcaa gctcagcctc gctcgcgagc tcaagaaagc
    10861 tcgcctgaaa aaggggcttt cccagacggc gctcgcgtcg aagctgggtt cgagccagtc
    10921 ccgggtcgcg aagatggagg ccgcggacga gcaggtcagc cttgatctga tcgtccggtc
    10981 gctccttgcc accgggaaac ggccccggga aatcgcccgc gccgtggcca agtagcccgc
    11041 gttcccatcg agtacggcta ccccgggccg gaatgcgggc cctcgaagcg gtgggcgaca
    11101 caccctaata gaccgccccc gggcgcgctc ctacttcttc ggcgcgctct tctccacggg
    11161 cagttcgctc ttgcggctcc actcgtccca cgagccgtcg tagaggagcg ccttgtagcc
    11221 gaggcgccgg gcggcgacgt acatgacggt ggcctgctgg ccgatgtggc agtaggagac
    11281 gacggtgtcg ccgggcttga tgcccgcggc ctccatcacc ttcgccgtgt cggccgggct
    11341 cttcatggtg ttgtcgtcgt tgacgagcgt gtcgaacggc agcgtcttcg cgccggggat
    11401 gtggcccgcg cgcggcatgt tgccggcgtc ctcgccgtcg tagaacttcg ggttgcgcga
    11461 gtcgacgatg gcgacgccgc tcttccggat gctcgccttg actaagtcga ggtccgcgac
    11521 gagctcgggg tgcggctttg cggtgatctt cccgggcttt ctctggacgg ccgacgcggg
    11581 gtcgttcgcg gacgcgacgg ccgggccgcc cgcggccttc cacgccggca tgccgccgtc
    11641 gaggatggag gtccgctcgc ccaggcccgc ggcgtcgagg ctgaagtaga cgcgcgtcgt
    11701 gggcgagacc cagtccttcg cgaagtagac gacgacgcgc gagtcgtcgg agatgccgta
    11761 gccctcgagc ttcgccttca gctggtcggg cgggaggagc tggaggatga gcgcgccatc
    11821 ggcgcgcgga aacgcgaagt cggcgggcgt gacggggatc gcgcccggga tgtgctcttt
    11881 ctcgaactcg gccggcacgc ccacgtgcag gaggacgagg ttcttgtcgc gctggtgcgc
    11941 ggcgagccac tccggcgtca cgaggatgtc cgccgcaggc gcggcggcgg aggcgaggaa
    12001 gaggagggcg gcgagggcgt tcttcatggg agcctcccgg ggcggccatt ctgcgccgag
    12061 cggcattccc ctgtcttggt cgagatgggg cttcgaggcg cgccgggcgc atgcgattct
    12121 gagcgcgcac gatgagcttc gcggatctct ggaccctcga cccggccgtc gactacctga
    12181 accacggctc gttcggggcc tgcccgaagg cgatcctcga gaagcagtcg gagctccgcg
    12241 cgcggctgga gcgcgagccg atggacttcc tcgtccggca cgcgcccggg ctgtacgcgg
    12301 acgcacgaac ggccctcgga cagttcgtcg gcgcggaccc ggacgacctc gcgttcgtcc
    12361 cgaacgcgac gacgggcgtc aacgcggtcg tgcggtcgct cgcgttctcc cccggcgacg
    12421 agatcctcac gacggaccac gcgtacggcg cgtgccggaa gacgctcgag tacgtcgcgg
    12481 cccgcgcggg cgcgcgcgtc acgatcgcga aggtgccgtt cccggtcgcg ggccccgacg
    12541 acgtcgtctc cgcggttctc tcggccgtga cgccgcgcac gcggctcgcg atgctggacc
    12601 acgtcacgag cccgacggcg ctcgtcttcc ccgtcgagcg gctcgtggcc gagctctccg
    12661 cgcgcggcgt ggacaccgtc gtggacggcg cgcacgcgct cgggatgctg ccgctcgacc
    12721 ttacgcgcct cggcgccgcg tactacacgg gcaacgcgca caagtggctc tgctcgccga
    12781 agagcgtcgc gttcctctac gtgcggaagg accggcagaa actcgtccac cccgtgacga
    12841 tcagccacgg ttacgcgggc ggcgaggcgc gcttccgcga cgagttcgac tggacgggga
    12901 cgatcgatcc gacgcccgcg ctgacgctcc cgttctgcat cgagtatctc ggcgggctgc
    12961 tgccgggagg ctggcccgcc ttgatggaga aaaaccggga cctcgcgctt cgcgcccggg
    13021 ggattctctt agaaaggctc ggggggacgc cggcgtgccc ttccgcgatg gtggggtcga
    13081 tggccgcggt gatcctgccg ccgctcccgc ccgggagccc tgcggcgcgc ctcgaccgcg
    13141 agcgcctggc ggactggatg cgggcgcgcg gctccgagcc gtggttcttc gggtggccgc
    13201 cggcgggagg cggcaagctc gtccgcgtct cggcgcagct ctacaacgcg gaagagcagt
    13261 acgtgcggct cgcgggcctc ctcgccgagg cgctgcgtga aggctgaggg gatagaagcc
    13321 cggctcggga cgttcgacac ggcgatggtc gtcgtctccc tcgtcgtggg gatcgggatc
    13381 ttccggacgc ccgcgatcgt cgcgaaggcc gccgggacgg aggggcgttt cctcctcgcg
    13441 tgggcgctcg ccggcctcgt gagcctcgtc ggggcgctcg tgttcgcgga gatcggctcg
    13501 cggcatccgc gcgcgggcgg ctactaccgc gtcgtcgccg actgttacca cccggcgctc
    13561 gcgttcatgc tgaactgggc gcagacgctc atgcagggcg cgggcgcggc gggcgtcgcg
    13621 ttcatcggcg ccgagtacct cgtggggctg ctgctgccgc cggagaagcg cggcggcagc
    13681 gccgtcctcc tcgtggcgct cgccctgatg ctcgtcctcc tcgtcctgaa cgggctcggc
    13741 atccgcgtgg gcgccggaac gcagaacgtc ctctcgatga cgaagatcgt gatgatcgcc
    13801 ggcctcgcgg gcgtcgcctt cgtcttcgcg ccgcgctttg cgggcgcctc cgccgctccg
    13861 tccgccgcgc ccgcgggcct cgccgcggcg ctgatcccgt gcttctacgc gtacggcggg
    13921 taccacatga cgatgaacct cggcgcggac gtgaaggacg ccaggcggcg gtttccgctc
    13981 gccgtcacct tcgggatgct cgtcgtggcc gggctgtacc tcgtcctgaa ctgggcgtac
    14041 gtgcgcacgc tcggggccgc gggcgtcgcg gggtcgaacc tcgtcgcggc ggcgctcgcg
    14101 cgcgcgagcc tgggagcggg aggtgaggcc gtggtgtcgg cgctcatatt cctgtcggcc
    14161 gccggcttcg tgaacgcgac cgtcctccag atgccgcgga gctaccacgc gatggccgag
    14221 gacggcgtgc tccccgcggt gttccggcgc gtggacccgc ggacgcaggt gtcgctcgcc
    14281 ggcctcgctc tcttcggcgc cacgatgctc gtgcccgcgt tcctccttgg ctccttcgag
    14341 aagctcctga actacatcat cttcacggac atgctgacgc tcgccgtcgt ggcgtcgacg
    14401 ctcttcgtgc tccggcgccg cggcgaggga gacggcgcct tccggatccc gggtttcccc
    14461 gtcctcccgg cgctctacgt cctcgcgctc gcggcagtcg ccgcgcacgt cgcggtgacg
    14521 gaaccggcgc tcgcgctggc ggggacgctc atccttctcg cgggcgggcc gctgttcgtc
    14581 ctcgggcgga agctgaaccg ggccgggtga agcagcctgc cgcttgacgc gggggcggcg
    14641 ggcttctacc ttgagaccgt ggccaaaccc gcgtcgcccc ccgcgctcat cgcggtgctc
    14701 acgtccgtgg gcaccgagca gcaggcgctc gacatcgcgc acgcgctcgt gaagaggcgt
    14761 ctcgccgcct gcgtgaacat cctgcccggc gtgcggtcgc tcttccgctg gaagggaaag
    14821 gtgcagcagg acgccgagct gcttctcgtc gtcaagacgc tcgcctcgaa cttcgaggcc
    14881 gtgcgcgggg tgatcaagga gctgaacgcc tacgagctgc ccgagatcgt cgccttcccc
    14941 gccgcgatgg ccgattccgc gttcgcggcc tgggtccacg actcgtcgac cggcgacgcg
    15001 agcgacgacg acgaggaaga cgagcccggc gaagcgacga tcgactgacg ggacgtttga
    15061 actgcgccgt gggttttcgg gcccgtgcga cactccccgc gccgccatcc ccgtcccgat
    15121 cccccggagg ttcgatgcgc cgactgcttc cctgcctgat cctcgccgcc gccctgccgg
    15181 gcgcgccgct ggccgccaac tcggtgaccg tgcagcccgt ggcggacacg tcgatctacg
    15241 aggagaacgg cgaccaatcc gacgcgaagg gccccgggat cttcgccggc cgcaacgtgc
    15301 tgacggccat ccgccgcgcc ttcgtgcggt tcgacgtcgc cgccgccgtg ccggcgggct
    15361 ccacgatcga cggggtgcgc ctcgacctcg tcctcctgag cgggaagggg aatccggtgg
    15421 acgtcaacct gttccgcgtg acggccgcct ggggtgaagg gacctcggac gccgggagcc
    15481 ccggcggcac gggcgcgccc gcgacgcccg gggacgccac gtggacccgg cgcatctggc
    15541 ccggcacggc gtgggtcacg cccggcgggg acaccgtggg cttcttttcc gcgaggatcc
    15601 ccgtgcagac gacgctgacg acgtactcgt tcggccccac ggccgcgatg caggccgacg
    15661 tgcagagctg gctggacgcg ccggcgacga acttcggctg gcaggtgcgc gccgacgagc
    15721 tgcaggcggc gccgagcgcg cggcggttcg gctcgcccga gtccgcgaac gccgcccagc
    15781 gccctcccct cacgatcccg ttcacgccgc ccgggggcgg gccgggcagt cggcccgcgg
    15841 acgacgtg
//
LOCUS       DAOMFY010000061        15778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1146_length_15778_cov_7.245309, whole genome shotgun sequence.
ACCESSION   DAOMFY010000061 DAOMFY010000000
VERSION     DAOMFY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1146_length_15778_cov_7.245309"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /gene="pyk"
                     /locus_tag="PLB01_07140"
     CDS             complement(<1..1242)
                     /gene="pyk"
                     /locus_tag="PLB01_07140"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQQ77115.1"
                     /translation="MSGKTGEGATKIVATIAGNAEKPGALDALLAAGVDVVRLNGAHA
                     KPGDIARRTALVRRVSARRGRPVGVLLDLGGPKIRLGALPGGSASLLVGHTVEIVAGA
                     VSAGGEKGGPLRLAVTYPALLKDVGPGAEIRIDDGRVRLEVTRRLRGLLRAVVRTGGR
                     VKSEAGVNFPDSTLTAPALTPKDRRDLDEALAAGVDFVGLSFVRSAAHVAQLRRVMTR
                     VPEDRRPWIVAKIERPEALRDLDRIAAAADALMVARGDLGVEIGLARVPAAQREILAA
                     GRRHAVPVIVATQMLESMIECPVPTRAEVSDVALAVHDGADAVMLSGETAVGAFPVEA
                     VRAMGEIAGAAEVPDAGSVPAPLPPPPPGDFTAVMARAAAEAARLGGASVLVVHTESG
                     RTARLASKNTLALPIVVFTPRE"
     gene            1276..1881
                     /gene="folE"
                     /locus_tag="PLB01_07145"
     CDS             1276..1881
                     /gene="folE"
                     /locus_tag="PLB01_07145"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQQ77116.1"
                     /translation="MAPSSILEPPVAADPRIDKLARHVREMIRILGLDPEKDANLVDT
                     DLRVARMYLEIFSGLDAGNRPKLTTFPNDEKYSAMVMEKDIPFYSMCAHHFVPFYGHG
                     HIAYIPNERIVGLSKLPRLLEFYARRPQLQERLTEQVASTLEEELKPQGVMVVVEARH
                     LCVEMRGVKKPGALTVTSALRGIFAQKAVREEFLDLLNRRG"
     gene            1930..2832
                     /locus_tag="PLB01_07150"
     CDS             1930..2832
                     /locus_tag="PLB01_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HQQ77117.1"
                     /translation="MSRAAAVPTVLAAALALALPAAAEHRHSMDVTVEERDAKGCAGV
                     HVRFGDRATARGEETVTLSRAEARGLVLEGSRNGGVTVRGGDANTFTVTACKAAAGDD
                     DASARAVLGQVALKSSGGRVTIEGPAGERWSGFLLVSAPRDADFSVEASNGPVSLRDL
                     SGTLAVTTANGPVSLSGLTGTVTVDAANGPVSLKNSSGDLRLHAVNGPLTIALAGTRW
                     EGRGLEADAKNGPLTLRLPDDFASGVRVETSHHAPFSCRAAACGSARRQDEGRTRLLE
                     FGGDASIRLTSANGPVSIVGSSRS"
     gene            complement(2846..3619)
                     /locus_tag="PLB01_07155"
     CDS             complement(2846..3619)
                     /locus_tag="PLB01_07155"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HQQ77118.1"
                     /translation="MRLVSLCPSITESLAALGAAGDLVGITRYCIHPQEALRDVPRVG
                     GTKNPDLAAIRAAKPDLVFCNAEENRGEDIAALRREFAVDVSHPRTVAEIPGLLQHFG
                     AAAGRGEEAEKISSKVEEALERAEEEARLTGLRFRVVYLIWKDPWMTIGPRTYVADLL
                     GRVGGRLALEESPGAGARDYPETSENAILLSRPDVLILPDEPYPFARKDAAFWRERLP
                     ASCRVVVVKGDDFCWHGVRTLRGLDAAVRLVRRLSESPA"
     gene            3698..5569
                     /locus_tag="PLB01_07160"
     CDS             3698..5569
                     /locus_tag="PLB01_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQQ77119.1"
                     /translation="MSSVLTEATAGAGSSLGVVVNDFSLQVATANGSGSQTANSVLMR
                     SIFQMGVPVSGKNLFPSNIQGLPTWFTIRASKHGYIGRKREIDVLVCMNPETAEEDVR
                     EARPGAHVVYEKKLGLENPGKKRDDVTYYPVPFAELVVKITAAPEHAKLRKLIVNMIY
                     VGVVADLMGIDPAESEIAIAKQLKGKKKAVDLNVSAVKLGLDYAAANFPAKIPYRVER
                     MNATAGKIIVEGNAAAALGCVFAGATVCAWYPITPSSSLCESFIDFCDELRVDPATGK
                     KNVAVIQAEDEIASIGMVVGAGWAGARAFTSTSGPGVSLMSEIIGLGYYTEVPAVIFD
                     VQRVGPSTGLPTRTMQGDVLLCYFNSHGDAKHPVLFPASPEECFTMAGDAFDLAERLQ
                     TPVFVLTDLDLGMNNWMSDAFPYPTAPLDRGKVLDAAAIEKVKDTWGRYKDVDGDAIP
                     YRTLPGTDSAAGAFFTRGSGHNEKAQYSEKAADYVNNVDRLSRKFDTARTLVPKPEIQ
                     DRPGAVAGVLAYGTSHYGTAEGRDKLAAEFGVPLDYCRLRALPVGPETAAWIRRHERI
                     YVVEQNRDSQLVTILRDEFPELAAKFVPIRQYNGLPLDATTVVEGVLSDRKTSGKKG"
     gene            5575..6633
                     /locus_tag="PLB01_07165"
     CDS             5575..6633
                     /locus_tag="PLB01_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQQ77120.1"
                     /translation="MSAQPPAPAPTPAAPKTNRIGLPKAQYEGSKSTLCLGCGHDVIT
                     KQVVNAFYEMGVPPYQVAKFSGIGCSSKTPAYFLNRAWGFNGVHGRMPAAATGALVAN
                     PELVGIGVSGDGDTASIGIGQFVHLVRRNLPLVYIVEDNGVYGLTKGQFSATADVGAT
                     RKKGDVNEFVPIDLCSLAIELGGTFVARSFSGDMKQLQTILEAAIAHKGTSIIDVISP
                     CVTFNDHEGSTKSYKYAKEHETPVHDVGFVPYFEDTLAEIPAGDTKDVPFPDGSVLRF
                     RGVGRDYDPTDKEKALRSIHESHGKQEILTGILYLNPDKPGFSEVLGATGKSLVNLPL
                     DVVRPSREALERVMESMR"
     gene            6630..6983
                     /locus_tag="PLB01_07170"
     CDS             6630..6983
                     /locus_tag="PLB01_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77121.1"
                     /translation="MSGATTSIRVFGAYLVILGAALIVAPNAVLAPFGVPSTSEVWVR
                     VVGVLAVALGFYYGQAARSEFVPFYRATIVMRAFVFVSFGAFVLLGFAPPALALFGTV
                     DLAGAIWTAAALKKG"
     gene            7000..7401
                     /locus_tag="PLB01_07175"
     CDS             7000..7401
                     /locus_tag="PLB01_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HQQ77122.1"
                     /translation="MPIRIVRLGSPRSPGEGPRIGTVRRPPRGVPKADFAKRDFYDVW
                     LPNLAPSEELLKAGQAAQDDAARWKAFARRYRAEMKQPDASRVLDLLAALSHGTNFAV
                     GCYCADESRCHRSILRELLKQRGARLSPGDP"
     gene            complement(7382..8251)
                     /locus_tag="PLB01_07180"
     CDS             complement(7382..8251)
                     /locus_tag="PLB01_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQQ77123.1"
                     /translation="MKRSKGVLRIEQAPPRHWVGDGFPVRSMFSYDSAPYLSPFILLD
                     YAAPAEFKPALEPRGVGEHPHRGFETVTIAYQGEIDHRDSAGHKGSIGPGDVQWMTAA
                     SGIVHEEMHGKEFTKKGGTLEMVQLWVNLPAKSKKAPPGYQDVVGGRTPVVALPGEAG
                     SVRVIAGEFEKAKGAARTFTPIEVWDLRLRAGKLANLNVPAGQSTALLFLHGGGRVNG
                     EKSVDEGQLVVLEREGETLSIEAASDATILLLSGTPIDEPVVGYGPFVMNTEKEIRQA
                     IADYRSGLMGHLD"
     gene            complement(8264..8437)
                     /locus_tag="PLB01_07185"
     CDS             complement(8264..8437)
                     /locus_tag="PLB01_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77124.1"
                     /translation="MSTPFLLGLVLILGALGIWYFRARSARDSRRASAASRARNNAVR
                     SAQSVEAAIRGRR"
     gene            complement(8467..11022)
                     /locus_tag="PLB01_07190"
     CDS             complement(8467..11022)
                     /locus_tag="PLB01_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HQQ77125.1"
                     /translation="MPDAAPLADRIPPRGTSDPDEILGLFLDWVASTGLTLYPAQEEA
                     LLEIMAGKHVILGTPTGSGKSLVALGLHFKAACEGRVSVYTSPIKALASEKFFSLCND
                     LGPENVGMRTGDASINPDASVVCCTAEVLSNMALRMGPALDVPYVVMDEFHYYADKER
                     GVAWQVPLLVLPGTQFLLMSATLGDTRAIAERLEKDTGRAVALVSSDERPVPLDFEYR
                     ETPLHETIEDLLAAKKSPIYVVNFTQRECAELAQSLTSVKIGTKEERDEIRKIAAGVR
                     LATPYGKEFRRFLSFGIGVHHAGLLPKYRLLVEQLAQKGLLHVVCGTDTLGVGVNIPI
                     RTVLFTKLAKFDGTKVGLLSVRDFKQIAGRAGRRGFDTQGSVVAQAPEHIVEKRQAEK
                     KSEVSGRKARPAKAPPKGEVSWTVETFQKLISRPPETLVSRFRLTHGMVLSLLQRDEA
                     EQPDRNFASLRDLVARCHEDEGTKRRLVKQAAVLVRSLYRAGILVLEGAGPYRVAVAR
                     DLQFDFSLHQALSLFALSALEELDPASPTYAVDVLTVVESILEDPDVILRRQTDREKG
                     RLVAQLKEEGVPYEERMERLEEVTYPKPLADFLYGAFNAFRAAHPWTAGSDVSPKSIG
                     REMFETFAGFSDYVKSYGLERSEGVLLRYLSQLYRTLDQTVPEPAKTDEVWDVLGFFR
                     SLVGETDASLLEEWERMRHPELLLAERAAPAAAARKPEATWLTELLANPRVFAARVRA
                     EAQLLVRALARKDWEEAADRVAPDASDPWDAARFEAALAPFFEEHGELLSGSEARLHH
                     LTRLTSAGPRRWEVAHTLLDPKGDNTWAVFAEIDLTDAGAPDGPILRIRRIGT"
     gene            complement(11028..11477)
                     /locus_tag="PLB01_07195"
     CDS             complement(11028..11477)
                     /locus_tag="PLB01_07195"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQQ77126.1"
                     /translation="MLKAARDVSSKARYATFVTIGPDGQPQARIIDPIGPDAEFAVWV
                     ATNPATRKVADLARDPRATLQFFEASLPAYVTLVGTASLVADPEVKALHWKDDWGPFY
                     KDKFRGADFVLLKFVPKRLEIVSQSHGLVNDPKTWRPVSAEFPATTP"
     gene            complement(11541..12359)
                     /locus_tag="PLB01_07200"
                     /pseudo
     CDS             complement(11541..12359)
                     /locus_tag="PLB01_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            complement(12386..>15778)
                     /locus_tag="PLB01_07205"
     CDS             complement(12386..>15778)
                     /locus_tag="PLB01_07205"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HQQ77127.1"
                     /translation="EQAVPISGGAATIARNMEASLGVPTATTNRVVPVKNMEENRRIL
                     NRHREALGLSKVSFTHFVAWAIVRALEKHPGMNDAFGEADGKPARIKKPSVNLGIAID
                     ITKKDGSRSLLVPNIKGAEKMSFGTFLAEFDALVGKARKGAIEPDAFAGTTISLTNPG
                     TIGTTSSLPRLMPGQGAIIATGAMGYPAEFQAMPDDVMSALGVSRVMNVSSTYDHRII
                     QGAESGGFVATLQDLLLGNDRFYDRIFEDLKVPHRPMRWEKDASSPFFGGTNRLEAVE
                     KQARVLQLINAYRVRGHLVADVNPLGVDAVPYHPDLDPATYGFTLWDLDRPFITNGLA
                     GRDHATLREILEVLRQTYCGKIGAEFMYNQDTAQKTWLIERMEGSRNRANLSREDRRR
                     ALNALVKAEAFEKFLHTKYVGQKRFSLEGGEAAIALLERLLDRAAEADLGEAVIGMSH
                     RGRLTVLVNNVGKPAGQIFAEFEGQVDPGTVQGSGDVKYHLGAVGTHVAPSGQKLGVS
                     LAPNPSHLEFVDPVVEGMVRAKQTALGDHREHSKVLPILLHGDAAFAGQGIVAETLNM
                     SQLHGYRTGGTVHVVVNNQIGFTTNPADARSSPYCTDVAKMVQAPVFHVNGDDVEAVL
                     HVADVAMEYRNTFRRDVVIDMVCYRRYGHNEGDEPSYTQPLMYVKIKTHTSVARLTGA
                     ALVKQGVFSQGEVDQMWAEAKTALDRAYDDARGGAKSGFDSLTAAPAKRLKPKGTVRE
                     RLAEVARAVSAVPEGFEVHPKLKPILKKRSEYASGRPEIDWSGGEMLAFGMLLLDGTP
                     VRLSGQDSGRGTFSQRHAVLADAATGAEWTPLNTIAPEQAAFEVIDSLLSENAVMGFE
                     FGYAVADPSTLVLWEGQFGDFANGAQVIIDQFISGSEQKWNQRCGLVLLLPHGQEGQG
                     PEHSSARLERFLTLCAEDNMRVANASTPAQYYHLLLRQMAGDVRKPLVVMTPKSLLRH
                     PKVVSTIDELADGAFHPVLDDPAGPSRARRVILASGKVAWDLIAAREKADRTDVAIVR
                     LEQYYPFPGREIGAVLQRYPQDAELVWVQEEPRNMGAWRFVREQFLDGKVEGLAAGRT
                     LRYIGRRELASPAPGSHHAFAHEQEALVAEALRVGARETAAV"
BASE COUNT         2296 a         5625 c         5496 g         2361 t
ORIGIN      
        1 ctcgcggggc gtgaagacca cgatcgggag ggcgagcgtg ttcttggacg cgagacgcgc
       61 cgtccggccc gattcggtgt ggacgacgag gaccgaagcg cctccgagac gcgcagcttc
      121 ggccgcggcg cgggccatca cggccgtgaa gtcgcccgga ggaggaggcg ggagcggggc
      181 gggcacgctc cccgcgtcgg gcacctcggc ggcgccggcg atctcgccca tcgcgcggac
      241 ggcctcgacc ggaaaggcgc cgaccgccgt ctcgcccgag agcatcacgg cgtccgcgcc
      301 gtcgtgcacc gcgagggcga cgtcggacac ttccgcccgc gtcgggacgg ggcactcgat
      361 catggactcg agcatctgcg tcgcgacgat gacgggcacc gcgtgccgcc ggcccgccgc
      421 gaggatctcg cgctgggcgg ccgggacgcg cgcgaggccg atctccacgc cgaggtcgcc
      481 gcgcgccacc atgagcgcgt ccgctgcggc ggcgatccgg tcgaggtccc ggagcgcctc
      541 gggacgctcg atcttcgcga cgatccaggg cctgcggtcc tccgggacgc gcgtcatcac
      601 gcgacgcagc tgcgcgacgt gcgccgcgct gcgcacgaac gagagcccga cgaaatccac
      661 ccccgcggcg agggcctcgt cgaggtcgcg ccggtccttg ggcgtgagcg ccggagccgt
      721 gagcgtcgaa tccggaaagt tcacgccggc ctcgcttttc acgcgacccc ccgtgcgcac
      781 gacggcgcgg agcaacccgc gcagccggcg cgtcacctcg aggcggacgc ggccgtcgtc
      841 gatccggatc tcggcaccgg gccccacgtc cttgaggagc gccggatacg tgaccgcgag
      901 ccgcaggggg ccgcccttct cgccccccgc ggacacggct cccgcgacga tctcgacggt
      961 gtggccgacc aggagggacg ccgagccgcc cggcagagcg ccgagacgga tcttcgggcc
     1021 tccgaggtcg agaaggacgc cgacgggccg cccgcgccgc gcggagaccc gtctcacgag
     1081 cgcggttcgc cgcgcgatgt cgccgggctt cgcgtgcgcc ccgttcagcc ggacgacgtc
     1141 cacccccgcg gcaagcagcg cgtcgagcgc ccccggcttc tccgcgttgc ccgcgatcgt
     1201 cgcgacgatc ttcgtcgccc cctctccggt ctttcccgac atccgtcctc ccctgccgcg
     1261 ccgtatgatg cctcgatggc cccctcctcg atcctcgaac cgcccgtcgc cgccgacccc
     1321 cgcatcgaca agctcgcccg ccacgtccgg gagatgatcc ggatcctggg cctcgacccc
     1381 gagaaggacg caaacctcgt cgacaccgac cttcgggtgg cgcggatgta cctcgagatc
     1441 ttctcgggcc tcgacgccgg gaaccggccg aaactcacga cgttcccgaa cgacgagaag
     1501 tacagcgcga tggtcatgga gaaggacatc cccttctact ccatgtgcgc ccaccacttc
     1561 gtaccgttct acgggcacgg ccacatcgcg tacatcccga acgagcgcat cgtggggctc
     1621 tcgaagctgc cgcgcctcct cgagttctac gcgcgcaggc cccagctcca ggagcgcctc
     1681 accgagcagg tcgcgtcgac gctggaagag gagctcaagc cccagggggt catggtcgtc
     1741 gtcgaggcgc gccacctctg cgtcgagatg cggggcgtca agaagcccgg cgcgctgacc
     1801 gtcacgtcgg cactccgggg catcttcgcc cagaaggccg tacgcgagga gttcctcgat
     1861 ctcctgaacc ggcgcggctg agaaaccttt ttcggcggcc ggcgtccccc tgagaacagg
     1921 aggaccccca tgagccgtgc cgccgccgtc ccgaccgtcc tcgccgccgc gctcgccctc
     1981 gctcttcccg cagccgccga gcaccgtcac tcgatggacg tcacggtcga ggagcgtgac
     2041 gcgaagggct gcgccggcgt tcacgtccgg ttcggcgacc gggccacggc ccggggcgag
     2101 gaaaccgtga cgctctcccg ggccgaggcg agggggctcg tcctcgaagg ttcccgaaac
     2161 ggcggcgtga ccgtccgcgg gggcgacgcg aacacgttca ccgtgacggc gtgcaaggcc
     2221 gccgccggcg acgacgacgc ttcggcccgg gccgtcctcg gacaggtggc tctcaagtcc
     2281 tcgggcggcc gcgtcacgat cgaaggcccc gccggcgaac gctggagcgg cttcctgctc
     2341 gtgagcgccc cgcgcgacgc cgacttctcc gtcgaggcgt ccaacggccc ggtctcgctg
     2401 cgggacctgt ccggaacgct cgccgtgacg accgcgaacg gccccgtctc gctctcgggc
     2461 ctcacgggca cggtcaccgt ggacgccgcg aacggccccg tctcgctcaa gaactcctcg
     2521 ggcgacctga ggctccacgc cgtcaacggc ccgctcacga tcgccctcgc gggcacgcgc
     2581 tgggagggca ggggcctcga ggcggacgcg aagaacggac cgctcaccct caggcttccg
     2641 gacgacttcg cgtccggcgt gagggtcgag acgtcccacc acgcgccgtt ctcgtgccgc
     2701 gccgcggcgt gcggatcggc ccgccgccag gacgagggcc gcacgcgcct gctcgagttc
     2761 ggcggggacg cctcgatccg gctcacgtcc gcgaacgggc ccgtctcgat cgtcgggtcg
     2821 agccggtcct agcgcccgcc ggccgtcacg ccgggctctc ggagagccgc cgcacgagcc
     2881 gcaccgccgc gtcgaggccc cggagcgtgc ggacgccgtg ccagcagaaa tcgtcgccct
     2941 tgacgacgac gacgcggcag gaggcgggga ggcgctcgcg ccagaacgcg gcgtccttcc
     3001 gcgcgaacgg gtagggctcg tcgggaagga tcaggacgtc cggacgcgag agcaggatcg
     3061 cgttctcgga tgtctccgga tagtcgcggg cgccggcgcc cggggattct tcgagtgcaa
     3121 gacggcctcc gacccggccc agcagatcgg ccacgtacgt gcgcgggcca atcgtcatcc
     3181 agggatcctt ccagatgaga tagacgacgc ggaagcgcaa tcccgtgagc cgcgcctcct
     3241 cctcggcgcg ctccagggcc tcttccacct tcgaggaaat cttctccgcc tcttctcccc
     3301 tccccgcggc agccccgaaa tgctgcagaa ggcccggaat ctccgcgacg gtcctcgggt
     3361 gcgacacgtc cacggcgaac tcgcggcgga gagccgcaat gtcctcgccg cggttttcct
     3421 cggcgttgca gaagacgaga tccggcttcg cggcacggat ggccgcgaga tcggggttct
     3481 tggtcccccc gacgcgcggg acgtccctca gcgcctcctg cgggtggatg cagtagcgcg
     3541 tgatcccgac gaggtcgccg gccgccccga gcgccgcgag gctctccgtg atcgacgggc
     3601 agagcgaaac gaggcgcacg gatcggattc ttccacgccc ggcttgaggc gggtaacatc
     3661 cctcggccgc ggccggtccg cgggaggagt ttcgtccatg tccagcgttc tgaccgaggc
     3721 caccgccggg gcgggttcgt ccttgggcgt cgtcgtcaac gacttctcac tgcaggttgc
     3781 cactgcgaac gggtccggtt cgcagacggc aaactccgtg ctcatgcgct ccatcttcca
     3841 gatgggcgtt ccggtgtccg ggaagaacct cttcccctcc aacatccagg gcctcccgac
     3901 gtggttcacg atccgggcga gcaagcacgg ctacatcggg cgcaagcgcg agatcgacgt
     3961 cctcgtctgc atgaaccccg agacggccga ggaagacgtg cgcgaggcgc gcccgggcgc
     4021 ccacgtcgtc tacgagaaga agctcggcct cgagaatccc ggaaagaagc gggacgacgt
     4081 cacctactac ccggtgccgt tcgccgagct cgtcgtgaag atcacggcgg cgcccgaaca
     4141 cgcgaagctc cgcaagctca tcgtgaacat gatctacgtg ggcgtcgtcg cggacctcat
     4201 ggggatcgac ccggccgagt ccgagatcgc catcgccaag cagctcaagg ggaagaagaa
     4261 ggccgtcgac ctgaacgtct cggccgtgaa gctcggcctc gactacgcgg ccgcgaactt
     4321 ccccgccaag atcccctacc gcgtcgagcg gatgaacgcg accgccggca agatcatcgt
     4381 cgagggcaac gcggccgcgg ccctcggctg cgtgttcgcg ggcgcgacgg tgtgcgcgtg
     4441 gtatccgatc acgccgtcct cctcgctgtg cgagtccttc atcgacttct gcgacgagct
     4501 ccgcgtcgac cccgcgaccg ggaagaagaa cgtcgcggtc atccaggccg aggacgagat
     4561 cgcctcgatc ggcatggtcg tcggcgccgg gtgggcgggc gcgcgggcgt tcacgtccac
     4621 ctcgggcccc ggcgtctcgc tcatgtccga gatcatcggc ctcggctact acaccgaggt
     4681 cccggccgtg atcttcgacg tccagcgcgt cgggccttcg acgggccttc cgacgcggac
     4741 gatgcagggc gacgtcctgc tctgctactt caactcgcac ggcgacgcga agcatcccgt
     4801 cctcttcccc gcttcgcccg aggagtgctt cacgatggcc ggcgacgcgt tcgacctcgc
     4861 cgagcggctc cagacgccgg tcttcgtcct gaccgatctc gacctcggca tgaacaactg
     4921 gatgtcggac gccttcccct atcccacggc gccgctcgac cgcgggaagg tcctcgacgc
     4981 ggcggcgatc gagaaggtca aggacacgtg gggacgctac aaggacgtcg acggcgacgc
     5041 gatcccgtac cggacgctgc ccggcacgga ctccgcggcg ggcgcgttct tcacgcgcgg
     5101 ctcgggccac aacgagaagg cgcagtactc ggagaaggcc gccgactacg tgaacaacgt
     5161 cgaccgcctc tcgcgcaagt tcgacaccgc gcggacgctc gtgccgaagc ccgagatcca
     5221 ggaccgcccc ggcgccgtgg ccggcgtcct cgcctacggc acgtcgcact acggcaccgc
     5281 cgagggacgc gacaagctgg ctgccgagtt cggcgtgccc ctcgactact gccgactccg
     5341 cgcgctgccc gtcggccccg agacggccgc gtggatccgg cgccacgagc gcatctacgt
     5401 cgtcgagcag aaccgcgaca gccagctcgt gacgatcctc cgcgacgagt tccccgagct
     5461 ggccgcgaag ttcgtcccga tccgccagta caacggcctc cccctcgacg cgacgaccgt
     5521 cgtcgagggc gtcctctcgg accgcaagac gtccgggaag aagggttgac cccgatgagc
     5581 gcgcaacctc ccgctcccgc ccccactccc gccgccccca agacgaaccg catcggcctc
     5641 ccgaaggccc agtacgaggg gagcaagtcc acgctgtgcc tcggctgcgg gcacgacgtc
     5701 atcacgaagc aggtcgtcaa cgcgttctac gagatgggcg tcccgccgta tcaggtcgcg
     5761 aagttctccg gcatcggctg ctcctcgaag acgccggcgt acttcctgaa ccgcgcctgg
     5821 ggcttcaacg gcgtccacgg ccgcatgccg gcggcggcaa cgggcgctct cgtcgcgaac
     5881 cccgagctcg tcggcatcgg cgtctcgggc gacggcgaca cggcctccat cggcatcggc
     5941 cagttcgtcc acctcgtgcg ccgcaacctc ccgctcgtct acatcgtcga ggacaacggc
     6001 gtctacggcc tcacgaaggg ccagttctcc gcaacggcgg acgtcggggc cacgcgcaag
     6061 aagggcgacg tgaacgagtt cgtcccgatc gacctctgct cgctcgcgat cgagctcggc
     6121 ggcacgttcg tcgcgcgctc gttctcgggc gacatgaagc agctccagac gatcctcgag
     6181 gcggccatcg cgcacaaggg cacgtccatc atcgacgtca tctcgccctg cgtgacgttc
     6241 aacgaccacg agggctcgac gaagagctac aagtacgcca aggagcacga gacgcccgtc
     6301 cacgacgtcg gcttcgtccc gtatttcgag gacacgctcg ccgagatccc cgcgggcgac
     6361 acgaaggacg tgcccttccc cgacgggagc gtcctgcgct tccgcggcgt cggacgcgac
     6421 tacgacccga ccgacaagga gaaggcgctc cggtcgatcc acgagagcca cggcaagcag
     6481 gagatcctca cgggcatcct gtacctgaac cccgacaagc ccgggttctc cgaggtcctc
     6541 ggcgccacgg gcaagtcgct cgtgaacctg ccgctcgacg tcgtcaggcc cagccgcgag
     6601 gccctcgagc gggtcatgga gtcgatgcga tgagcggagc caccacgagc attcgcgtgt
     6661 tcggggccta cctcgtcatc ctcggcgccg cgctcatcgt ggcgccgaac gcggttctcg
     6721 cccccttcgg agtcccttcg acgtccgagg tctgggtccg agtcgtgggc gtcctcgcgg
     6781 ttgccctcgg cttctactac ggccaggcgg cgcgttccga attcgtaccg ttctaccggg
     6841 cgacgatcgt catgcgcgcc ttcgtcttcg tctcgttcgg cgcgttcgtc ctcctcggct
     6901 tcgcgccgcc ggccctggcc ctcttcggca ccgtggacct cgccggagcg atctggacgg
     6961 cggctgccct caagaagggc tgagcccgga ggcgacgcca tgccgatccg catcgtccgg
     7021 ctcggaagcc cgcgcagtcc gggtgagggg ccgcggatcg gcaccgtgcg ccggcctcct
     7081 cgcggagttc cgaaggcgga cttcgcgaag cgcgacttct acgacgtctg gctgccgaac
     7141 ctcgcgccga gcgaggagct cctgaaggcc ggtcaggccg cgcaggacga cgcggcccgc
     7201 tggaaggcgt tcgcgcggcg ctatcgcgcc gagatgaagc agcccgacgc cagccgcgtc
     7261 ctcgacctcc tcgccgccct ctcgcacggg acgaatttcg cggtcggctg ctactgcgcc
     7321 gacgagagcc gctgccaccg gtcgattctg cgagagctgc tgaagcagcg cggcgcccgg
     7381 ctcagtccag gtgacccatg agcccgctgc ggtagtccgc gatcgcctgc cggatctctt
     7441 tctcggtgtt catcacgaac ggcccgtagc ccacgaccgg ctcgtcgatc ggagtccccg
     7501 acaggaggag gatcgtggcg tccgaagccg cttcgatcga gagcgtctcg ccctcccgct
     7561 cgaggacgac gagctgcccc tcgtccaccg acttctcgcc gttgacgcgc ccgccgccgt
     7621 gcaggaagag gagcgccgtg gactggcccg ccggcacgtt caggttcgcg agcttccccg
     7681 cccggagacg caggtcccac acctcgatcg gcgtgaacgt ccgggccgcg cccttcgcct
     7741 tctcgaactc gcccgcgatc acgcgcacgg agccggcctc gcccgggagc gcgacgacgg
     7801 gcgtgcggcc tccgacgacg tcctggtagc cgggcggggc cttcttggac ttcgcgggca
     7861 ggttcaccca gagctgcacc atctcgagcg tgccgccctt cttcgtgaac tccttcccgt
     7921 gcatctcctc gtgcacgatg cccgaggccg cggtcatcca ctggacgtcg ccgggtccga
     7981 tcgagccctt gtgccccgcc gagtcgcggt ggtcgatctc gccctgatac gcgatcgtca
     8041 cggtctcgaa accgcggtgc gggtgctcgc ccacgccgcg cggctcgagg gccggcttga
     8101 actccgccgg cgccgcgtag tcgagaagga tgaacgggct gaggtacggg gcggagtcgt
     8161 acgagaacat cgaacggacg ggaaacccgt cgccgaccca gtgccggggc ggcgcctgct
     8221 cgatcctgag aacgcctttc gaacgcttca agagcgttcg cggctagcgc cgccctctga
     8281 tcgccgcttc gacgctctgg gcgctgcgga ccgcgttgtt gcgggcacgg ctcgcggcgg
     8341 aggcgcggcg ggaatcgcgg gcggatcggg cgcggaaata ccagatcccg agagcgccga
     8401 ggatgaggac gaggccgagg agaaagggag tagacacggg caccacctga cgcaggatag
     8461 ccctcttcag gttccaatgc gccggattct caggatcggg ccgtcgggcg cgcccgcgtc
     8521 cgtcaggtcg atttcggcga aaaccgccca cgtgttgtcg cccttcgggt cgaggagcgt
     8581 gtgcgcgacc tcccaccggc gcgggcccgc ggacgtcagc ctcgtcaggt ggtggagccg
     8641 ggcctcggag ccggagagaa gctcgccgtg ctcctcgaag aacggcgcga gcgcggcctc
     8701 gaagcgcgcg gcgtcccatg ggtcgctcgc atcgggggcg acgcggtcgg cggcctcctc
     8761 ccagtccttc cgcgcgagcg cccggacgag gagctgcgcc tcggcgcgga cgcgcgcggc
     8821 gaagacgcgc gggttcgcga gaagctcggt gagccaggtc gcttcgggct tcctcgccgc
     8881 tgcggcgggc gcggccctct ccgcgaggag gagctccgga tggcgcatcc gctcccattc
     8941 ctcgaggagg ctggcgtcgg tctccccgac gagcgaccgg aagaacccga gcacgtccca
     9001 gacctcgtcg gtcttcgcgg gctccggcac cgtctggtcg agcgtccggt agagctgcga
     9061 caggtacctc aacaggacgc cctcgctgcg ttcgaggccg tagctcttca cgtagtccga
     9121 gaagcccgca aacgtctcga acatctcgcg cccgatcgac ttggggctga cgtccgagcc
     9181 cgcggtccac gggtgcgccg cgcggaacgc gttgaaggcg ccgtacagga agtccgcgag
     9241 cggcttcggg taggtgacct cctcgagccg ctccatccgc tcctcgtacg ggacgccttc
     9301 ttccttgagc tgcgcgacga ggcgtccctt ctcccggtcc gtctgccgcc ggaggatcac
     9361 gtccgggtcc tcgaggatcg actccacgac ggtgaggacg tccacggcgt acgtcggcga
     9421 ggcgggatcg agctcctcga gcgccgagag cgcgaagagc gagagcgcct ggtggagcga
     9481 gaagtcgaac tggagatccc tggccacggc gacccggtac ggccccgcgc cctcgaggac
     9541 gaggatcccg gcccggtaga gcgagcgcac gaggacggcc gcctgcttca cgagccgtcg
     9601 cttcgtcccc tcgtcctcgt ggcagcgcgc gacgaggtcg cgcagcgacg cgaagttgcg
     9661 gtccggctgc tcggcttcat ccctctgcag gagcgacagc accatcccgt gcgtcagccg
     9721 gaacctcgag acgagcgtct cgggcggccg cgagatcagc ttctggaacg tctcgacggt
     9781 ccaggagacc tcgcctttcg gcggagcctt cgcgggacgt gctttccttc cggaaacctc
     9841 ggatttcttc tcggcctggc gcttctcgac gatgtgctcg ggcgcctgcg cgacgacgct
     9901 cccttgcgtg tcgaagccgc ggcggcccgc gcgccccgcg atctgcttga agtcgcggac
     9961 ggacaggagc ccgaccttcg tcccgtcgaa cttcgcgagc ttcgtgaaga ggaccgtccg
    10021 gatcgggatg ttcacgccga cgccgagcgt gtcggtcccg cacacgacgt ggaggaggcc
    10081 cttctgggcg agctgctcga cgaggaggcg gtacttcggg aggaggccgg cgtggtgcac
    10141 gccgatcccg aaagacagga acctccggaa ctccttgccg tacggggtcg cgaggcggac
    10201 gcccgcggcg atcttccgga tctcgtcgcg ctcctccttc gtcccgatct tcacgctcgt
    10261 gagcgactgc gcgagctcgg cgcactccct ctgcgtgaag ttcacgacgt agatcgggct
    10321 cttcttcgcg gcgaggaggt cctcgatcgt ctcgtggagc ggcgtctcgc ggtactcgaa
    10381 gtcgagcggg accgggcgct cgtcggacga cacgagtgcc accgcacggc cggtgtcctt
    10441 ctcgagccgc tcggcgatcg cgcgcgtgtc gccgagcgtc gcggacatga ggaggaactg
    10501 ggtgccgggc aggacgagga gcggcacctg ccacgcgacg ccgcgctcct tgtccgcgta
    10561 gtagtggaac tcgtccatca cgacgtacgg gacgtcgagc gcgggcccca tccggagggc
    10621 catgttcgag aggacctccg ccgtgcagca gacgacgctc gcgtccgggt tgatgctcgc
    10681 gtcgcccgtc cgcatgccga cgttctcggg cccgaggtcg ttgcagagcg agaagaactt
    10741 ctcgctcgcg agcgccttga tcgggctcgt gtagaccgag accctcccct cgcaggcagc
    10801 cttgaagtgc aggccgagcg ccacgaggga cttcccggag cccgtgggcg tcccgaggat
    10861 gacgtgcttg cccgccatga tctcgaggag cgcctcctcc tgcgccgggt agagcgtcag
    10921 acccgtggag gccacccagt cgaggaagag gccgaggatc tcgtcggggt cgctcgtccc
    10981 gcggggcgga atccggtccg cgagcggcgc tgcgtcgggc atccgggtca gggcgtcgtc
    11041 gcggggaact cggccgagac cggccgccac gtcttcgggt cgttcacgag cccgtgggac
    11101 tggctgacga tctcgagcct cttcgggacg aacttcagga ggacgaagtc cgcgccgcgg
    11161 aacttgtcct tgtagaacgg cccccagtcg tccttccagt gcagcgcctt gacttccggg
    11221 tccgcgacga gactcgcggt cccgacgagc gtgacgtatg ccggaaggga ggcttcgaag
    11281 aactgaagcg tggcgcgcgg gtctcgcgcg aggtcggcca ccttgcgcgt cgccgggttc
    11341 gtggcgaccc acacggcgaa ttcggcgtcc ggcccgatcg ggtcgatgat gcgggcctga
    11401 ggctggccgt cggggccgat cgtgacgaac gtggcgtatc gcgccttcga agagacatcc
    11461 cgtgccgcct tcaggatcgc cgagcgctcc gctacgggaa gcggtgagga gggcgcggcc
    11521 gtggagaccg ggagcgccgt cgcgagcgcc gccgcttcgg ccgggcagtc gcgatcgggc
    11581 acggcgccct tggcgcgcac cgcggcgagc tccttcttcg cggccgcgag gtcggcgagg
    11641 aacgcggcgt ccccgtggag gcgggcgacg gtggcggcgc cgaggatgcg cccctcgttg
    11701 acgtccgcct gccagtggac gttgcagacg acgcggctct gcccgaacgc gcggccgcgc
    11761 tcgaccaccg cctcggcgcg atccggcgcg acctcggcga ggacgagccc ccacgcccag
    11821 ccggcggcgc tgtggccgga cgggaaagac ccctgcttcg cgagctcctt ctcgtcggcc
    11881 ggggtgcacg tcggctcgcc gttgacgagg aagggccgct tgcgcatgta cttgttcttg
    11941 gcggcgcccg tcgagtagcc ggcgtcggaa aggacgcgcc ggagaagggc gtagaggtgc
    12001 ggcgtgtcct gctcggtgat cggcgcgccg agcgcgcacg cgagggagcc ggcggcacgc
    12061 gggaacgtga ggttcgcgtc ctcggtcgcc tgcgcgaagc gcggcgtccc gcgcagagca
    12121 cgggccgcgc gatttgcctc atcgtccgcc gcgagcgccg cggatcccgt cgcgggcggc
    12181 ggcgggacga gcgcgaggct gtcgatggcc tcttctttcg tcaggtatcc cgcgatcgag
    12241 ccgggccaga tcggcgggac cgtcccgcgc gcctcgggcg gcggcggcgc cgtcgcgcag
    12301 ccggcggcga ggacgagagc gacgaagagg gccgggacgc gcatccggat gttcttcatg
    12361 cgcgaaagta tggcctaccc gagggttaga ccgccgccgt ctcccgcgcg cccacacgga
    12421 gcgcctcggc gacgagcgcc tcctgctcgt gcgcgaacgc gtggtgcgag ccgggcgcgg
    12481 ggctggcgag ctcgcgcctt ccgatgtatc ggagcgtccg gccggccgcg aggccctcga
    12541 ccttgccgtc caggaactgc tcgcgcacga aacgccacgc gcccatgttg cgcggctcct
    12601 cctgcaccca gacgagctcg gcgtcctgcg ggtagcgctg gaggaccgcg ccgatctccc
    12661 ggccggggaa cgggtagtac tgctcgaggc gaacgatcgc gacgtcggtg cggtccgcct
    12721 tctcgcgcgc cgcgatgagg tcccacgcga ccttgccgct cgcgaggatc acccggcgcg
    12781 cgcgcgacgg gcccgccggg tcgtcgagga cgggatggaa cgcgccgtcg gccagctcgt
    12841 cgatcgtcga gacgaccttc gggtggcgca gcaggctctt cggcgtcatg acgacgagcg
    12901 gcttcctcac gtcgccggcc atctggcgga gcagcaggtg gtagtactgc gcgggcgtcg
    12961 aggcgttcgc gacgcgcatg ttgtcctcgg cgcagagcgt caggaagcgc tcgaggcgcg
    13021 cggacgagtg ctcggggccc tggccttcct gcccgtgcgg cagcagcagg acgaggccgc
    13081 accgctggtt ccacttctgc tcggagcccg agatgaactg gtcgatgatg acctgggcgc
    13141 cgttcgcgaa gtcgccgaac tgcccttccc acaggacgag cgtcgacggg tccgcgaccg
    13201 cgtagccgaa ctcgaagccc atgacggcgt tctcggagag gaggctgtcg atgacctcga
    13261 acgcggcctg ctcgggcgcg atcgtgttca gcggcgtcca ctcggcgccc gtcgcggcgt
    13321 ccgcgaggac ggcatggcgc tggctgaagg tgccgcggcc cgagtcctgc ccggagaggc
    13381 gcacgggcgt gccgtcgagg agcagcatcc cgaacgcgag catctcgccg cccgaccagt
    13441 cgatttcggg ccggccgctc gcgtactcgg agcgcttctt gagaatgggc ttgagcttcg
    13501 ggtggacctc gaagccctcg ggaaccgccg agacggcccg ggcgacctcg gcgaggcgct
    13561 cgcgcacggt gcccttcggc ttgaggcgct tcgcgggcgc ggccgtcagc gagtcgaacc
    13621 cggacttcgc gcctccgcgg gcgtcgtcgt aggcgcggtc gagcgccgtc ttggcctcgg
    13681 cccacatctg gtcgacctcg ccctgagaga agacgccctg cttgacgagc gcggcgcccg
    13741 tgaggcgcgc gaccgacgtg tgcgtcttga tcttcacgta catgaggggc tgcgtgtagc
    13801 tcggctcgtc gccctcgttg tggccgtacc ggcggtagca gaccatgtcg atcacgacgt
    13861 cgcgccggaa cgtgttgcgg tactccatcg cgacgtccgc gacgtggagc acggcctcga
    13921 catcgtcgcc gttcacgtgg aagacgggcg cctggaccat cttcgccacg tccgtgcagt
    13981 acggcgacga gcgcgcgtcg gccggattcg tcgtgaagcc gatctggttg ttcacgacga
    14041 cgtgcacggt gccgcccgtc cggtagccgt ggagctgcga catgttgagc gtctcggcca
    14101 cgatgccctg tcccgcgaag gcggcgtcgc cgtggaggag gatcgggagg accttcgagt
    14161 gctcgcggtg gtcgccgagc gcggtctgct tggcgcggac catgccctcg acgacggggt
    14221 cgacgaactc gagatggctc gggttcggcg cgagcgagac gccgagcttc tgtccggagg
    14281 gggcgacgtg cgtgcccacg gcgccgaggt ggtacttcac gtcgccggat ccctgcacgg
    14341 tgccggggtc cacctgcccc tcgaactccg cgaagatctg ccccgccggc ttgccgacgt
    14401 tgttcacgag gacggtcagg cgcccgcggt gcgacatccc gatcacggcc tcgccgaggt
    14461 cggcctcggc ggcgcggtcg aggaggcgct cgaggagcgc gatcgcggcc tcgccgccct
    14521 cgagcgagaa gcgcttctgg cccacgtact tcgtgtggag gaacttctcg aacgcctcgg
    14581 ccttcacgag cgcgttcagg gcgcggcggc ggtcctcgcg cgagaggttc gcgcggttgc
    14641 gcgagccctc catgcgctcg atgagccacg tcttctgcgc ggtgtcctgg ttgtacatga
    14701 actccgcgcc gatctttccg cagtaggtct ggcggaggac ctcgaggatc tcccgcagcg
    14761 tcgcgtggtc gcgcccggcg agcccgttcg tgatgaacgg gcggtccagg tcccacagcg
    14821 tgaaaccgta cgtcgcgggg tcgaggtccg ggtggtacgg cacggcatcc acgccgagcg
    14881 ggttcacgtc cgcgacgagg tggccgcgca cgcggtaggc gttgatgagc tggaggacgc
    14941 gtgcctgctt ttccaccgcc tcgaggcggt tcgttccgcc gaagaacgga gagctggcgt
    15001 ccttctccca gcgcatcggc cggtgcggga ccttcaggtc ctcgaagatg cggtcgtaga
    15061 agcggtcgtt cccgaggagg aggtcctgga gcgtcgcgac gaacccgccg gactcggcgc
    15121 cctggatgat ccggtggtcg tacgtcgacg acacgttcat gacccgcgac acgccgagcg
    15181 cggacatgac gtcgtcgggc atggcctgga actcggccgg gtagcccatc gcgcccgtcg
    15241 cgatgatggc gccctgcccc ggcatgagcc gcggcagaga ggacgtcgtc ccgatcgtgc
    15301 cggggttcgt gagcgagatc gtcgtcccgg cgaaggcgtc gggctcgatc gcgcccttcc
    15361 gcgccttccc gacgagcgca tcgaactcgg ccaggaacgt gccgaacgac atcttctcgg
    15421 cgcccttgat gttcgggacg agcagcgagc gcgatccgtc cttcttcgtg atgtcgatcg
    15481 cgatgccgag gttcacggac ggcttcttga tgcgggcggg cttcccgtcc gcctcgccga
    15541 aggcgtcgtt catccccggg tgcttctcga gggcgcgcac gatggcccac gcgacgaaat
    15601 gcgtgaagga gaccttcgag aggccgagcg cctcgcggtg gcggttcagg atccgccggt
    15661 tctcctccat gttcttcacg gggacgacgc ggttcgtcgt ggcggtgggg acgccgagcg
    15721 acgcctccat gttccgggcg atcgtcgcgg cgccgcccga gatggggacg gcctgttc
//
LOCUS       DAOMFY010000062        15732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1152_length_15732_cov_8.357658, whole genome shotgun sequence.
ACCESSION   DAOMFY010000062 DAOMFY010000000
VERSION     DAOMFY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15732
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1152_length_15732_cov_8.357658"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..348)
                     /locus_tag="PLB01_07210"
     tRNA            complement(275..348)
                     /locus_tag="PLB01_07210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Gln,seq:ctg)
     gene            complement(406..801)
                     /locus_tag="PLB01_07215"
     CDS             complement(406..801)
                     /locus_tag="PLB01_07215"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQQ77128.1"
                     /translation="MRSRRRLLLWAPVVLLLAYEFWLSGRPPSGLPPLGPWFTGKDKV
                     EHTAYFLLTGLLAVRAARFGEGWSGAKTAVFLLLGAALWGCSDEIRQSFVPGRDVEIA
                     DVLADVAGVGFAVASGEWILRTSGLERRA"
     gene            complement(798..2507)
                     /locus_tag="PLB01_07220"
     CDS             complement(798..2507)
                     /locus_tag="PLB01_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HQQ77129.1"
                     /translation="MDDTKTPAPPAQGKDFIREIVDADAASGKHGGRVATRFPPEPNG
                     YLHIGHAKSICLNFGIAKEYGGTCNLRFDDTNPLKEEVEYVDSIQADVKWMGFDWEDR
                     LFYASDYFEKLYEFAEALIGKGKAFVCDLTADEVRATRGTLTEPGTNSPYRDRTPAEN
                     LDLFRRMRAGEFPDGARTLRAKIDMASPNINLRDPALYRIRKTVHHRTGDAWHLYPMY
                     DYAHAISDWIERITHSLCTLEFEDHRPLYDWCLEALDLPDRPRQIEFARLNVTYTLLS
                     KRKLLHLVTGKHVTGWDDPRMPTLAGFRRRGYTPEAIREFCDRIGLAKRNSVVEVELL
                     EHCLREDLNRRAPRFMGVLDPVKVVLVNWPEGKVEMLDAVNNPEDASQGTRAVPFSGT
                     LYIDRDDFREVPPPKYHRLSPGTEVRLRWAYVIRCTDVVKDAAGAVTELRCTYDAATK
                     GGEAPGRKVKGTIHWVSAAESLPAEARLYDRLFTAPNPDEAPEGKDFLANLNPDSLVV
                     RSGARVEPALAALPAGTAFQLERTGYFVVDPDSAPGRLVFNRSVSLRDSWGKIEKKAA
                     AAG"
     gene            complement(2516..3937)
                     /gene="gltX"
                     /locus_tag="PLB01_07225"
     CDS             complement(2516..3937)
                     /gene="gltX"
                     /locus_tag="PLB01_07225"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQQ77130.1"
                     /translation="MTAPRVRFAPSPTGSLHVGGARTALYNWLFARRHGGTFILRIED
                     TDVERSRKELTQQILDAMTWLGLSWDEGPFFQSERQDLYREAAERLLAEGKAYRAFET
                     PEELDAQKKAAEAEGSSFKYDGAARAIPKEESDRRAAQGEKFVLRLRVPDEPLVVDDV
                     VQGRSEFPAGALDDFVLVRSDGHPLYHFTVCVDDVAMGITHVVRGVDHLPNAPKHVAL
                     FRALGAPCPVFAHLGMILGADGRKLSKRHGAAGVEEFRDQGFLPEALVNFLALLGWSP
                     GEDRERMTLAEMTALFALERIGPSPSRFDHEKLQWMNGQYILDTPDARLLELLAPFGL
                     PARDPETLAKGVALLRPRAKTLVELAAGLSVYGDDPAVFDPAGLKKFGKPELAPVLRA
                     LAERLDAVDPFRHAGMEEAARALAAETGLGLGQIAQPARLAITGTLASPPLFEMIEVL
                     GRETARRRILAFADRIAAPAAPN"
     gene            3984..4904
                     /locus_tag="PLB01_07230"
     CDS             3984..4904
                     /locus_tag="PLB01_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQQ77131.1"
                     /translation="MSAGPRSVPRREALTADLVLVVVTAIWGSTFIVNRVVIQSAPPL
                     LFVLARFALAAAILWLIARGRPRTPNLVRDSAAVGLLLAAGIGFQVVGQLFTTASKAA
                     FVTGLSVPLTPVAGYLMTRKKPSRENLAGLVIAALGFAVLTWPKDASAGFNTGDLLIL
                     VTAVSYAVLIVIVSETAGTHDVRWYSFGQIVFAAIGVGAVRLALTPFVHGSGAFLAAE
                     ARPLVLGRTVVLAILWMALVATVVTFLAQTWAQARMPATHAAIMFALEPVFTALFAAF
                     FLGERMSRRDWAGAGLVLAGILVSELRGRD"
     gene            complement(4906..6198)
                     /locus_tag="PLB01_07235"
     CDS             complement(4906..6198)
                     /locus_tag="PLB01_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQQ77132.1"
                     /translation="MKSTRLAFALAALAASLPLSAAGREERRTPIVAAIEKISPSVVN
                     ISAERVVQRRSPVDLFFGGESRPRTSRSESLGSGVVIAPGVVVTNDHVISGASRIWVT
                     TADGRELEAKLEGADADNDLAVLSVDPKGLKPAALGTTSDLMIGETVIAIGNPFGLTN
                     TVTTGIVSAVHRTVKGESGRNYSDFLQTDAAINPGNSGGALVNILGELIGINTAIVGG
                     ANTIGFAIPIDRVRRVADDLLNYREVKTAWIGVRGTTLPPANGRPSARGQGLRVRSVW
                     PGSPAAKAGLAPGDVVVSLDGRPVEGREDFDTTLASVGPGKTLAVVYRREGADRTARV
                     TTERTPEDLGLDVLRRELGLVFKETRAGLMIASVLSRSPAAAKGLDRGDVVLAVNNVK
                     VRTLDDLSRAVESGWARPSLVLSVLREPYVYQMTFGLE"
     gene            6095..6661
                     /locus_tag="PLB01_07240"
     CDS             6095..6661
                     /locus_tag="PLB01_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQQ77133.1"
                     /translation="MAATIGVRRSSRPAAERGSEAASAASANARRVDFILRSSIIKSI
                     YRKGILSILNTRQKKAVQALVELVYRAGPDVPSGQLATWLDLSRDSVHQLLLPLVRNG
                     WVAAGRGRNGGYRATPVAPGVTVLSVVSLYSREGRDPTPDDPAAPRFIRNLDQRASER
                     YRQYLSSVTVGGLVAEVRAEREEPTYSI"
     gene            6727..7692
                     /gene="cysK"
                     /locus_tag="PLB01_07245"
     CDS             6727..7692
                     /gene="cysK"
                     /locus_tag="PLB01_07245"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQQ77134.1"
                     /translation="MKIASDITKLVGGTPLVRLNRVTAGIASGATVAAKLEFFNPAHS
                     VKDRIGVAMIEALEKQGKIAPGKSVLIEPTSGNTGIGLAFVAAAKGYRLILTMPETMS
                     IERRKVLKLLGAEVILTPGPLGMKGAIAKAQELLAEYGVNGVQPQQFENPANPAIHEA
                     TTAVELWNDTDGKMDIFVAGVGTGGTMTGVGRYWKPKKPAVRFVAVEPVHSPVISGGA
                     PGPHKIQGIGAGFIPKNLDTTFVDETFQVSNDQSFAMARRLAKEEGILGGISSGAAVH
                     AAVEIAKRPESAGKLIVCVVPSTSERYISTDLFKDDEVPAGAAAI"
     gene            complement(7744..8226)
                     /locus_tag="PLB01_07250"
     CDS             complement(7744..8226)
                     /locus_tag="PLB01_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQQ77135.1"
                     /translation="MPVIRWNDPFRDLATLQDRLNRAFDSTLARGGREQQEELFTGTW
                     APPVDIFETKEKTVLKAELPGFDENQIALRFEDGILSLEGERKFEKASDDENYHRIER
                     SYGKFVRSFTIPANVDNEKIVASFHNGVLTVELPKREETKPKAIKIQAAKPTIEAKKS
                     "
     gene            complement(8246..10864)
                     /gene="clpB"
                     /locus_tag="PLB01_07255"
     CDS             complement(8246..10864)
                     /gene="clpB"
                     /locus_tag="PLB01_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HQQ77136.1"
                     /translation="MNLDRFTHKAQEVLQKTAQSARAHGHPEVTPEHLAAALLEQEGG
                     VVPALLERVGVAPGHAAQEVAKLLAKLPHVAGENAEPRFSPALVKLLDLAEKIASDFK
                     DDYVASEHLFLALLKDGRSASAKAFLALGLSEASVLAALKDVRGGSRVADQGAEDKYQ
                     ALKKYAKDLTELARKGKLDPVIGRDEEIRRVMQVLTRRTKNNPVLIGDPGVGKTAIVE
                     GLARRIVSGDVPETLKDKRVVSLDLGFMLAGAKYRGEFEERLKAVLKEVEESDGKVIL
                     FIDELHTLVGAGAAEGAMDAGNMLKPALARGELRAIGATTVGEYRKHIEKDPALERRF
                     QPVLVGEPSVEDTVAILRGLQERYELHHGVKITDAALVAAATLSDRYIADRFLPDKAI
                     DLIDEAASKLKMEIDSMPTAIDEVERRILQIEIERKALQKDDAKAAKERLKGLEKTLA
                     ELKERSNAMKTRWRAEKDAITVIRDLKKKLDETKVAAEAAERQGDLNKAAELRYGTLL
                     KVQKDLEAAQARLDEMQKGGAILREKVDEDDVAAIVSKWTGVPVSRMVEGETQKLLKM
                     GDRLRLRVIGQDEAVDAVAAAVRRARAGMKDPRRPIGSFLFLGPTGVGKTELARALAE
                     FLFDDENAMVRLDMSEYQEKHTVSRMIGAPPGYIGHDEGGQLTEAVRRRPYSVVLFDE
                     IEKAHPDVWSTLLQVLDDGRLTDGKGRVVDFKNVVVVMTSNVGSHLIQELQGVDEKAL
                     KARIETELRAHFRPEFLNRIDDIVTFRRLELSDIEKIVEIQLRKVAELVAPRGIKLAW
                     EPPVATWLAREAFDPVYGARPLKRNIQKLVQDPLAWKLLAGEVRDGSAVTLALAPGGS
                     ALEFRTEPAEAPAKKR"
     gene            10996..12774
                     /locus_tag="PLB01_07260"
     CDS             10996..12774
                     /locus_tag="PLB01_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77137.1"
                     /translation="MGRRGAGRREDGRGWPRFIGIFLFAFGFRFFVSSSLSHLGLWQS
                     PQFDAHENLAWAHALAAGEFQWPSPPTHGPAYPFLLALLLKVFGTVSAARVAQAALAS
                     ATCVLAARTGELLFERRAGLAAGVLLAVSGPVALVDVSFWEEGFVSFLLSAALLLLVA
                     RRTPAFAGASGLLLGIACAARPTAVLFVLAALAFVLFLEGSSRPGPTAAALLLGVAVV
                     LLPAVIGSSRAAGHFVFVRAYGAVNLWIGNDPAGGGVQNARPNGPWDRLAAEPARAGV
                     APKDEEAWFTRRAIERATADPVGLARVVLSKLAWLTQAEEPRDNHAFAFFASQSGILR
                     ILPGFGLLFAMAAAAFFARLPLRTAGLPLLWIVAGVLPFLAALAGLRYRMPVVPAVAL
                     FAGAGAAHLLDRVKKRDLRLVAAPAVVVALTLCASHVRRHPPSHVFAEEWALEGNAWM
                     ELGKPAEAERTLRRAVEADPKAALPVEFLGRLRLAQGRAAEAADTFEESLRMDPDSRS
                     AHFFLGQAREALGLWPGAASAYRAALAISPAFFPAQFHLGRALLLAGDPAGAVAALEK
                     AVALQPLEQPPRELLEKARALALSPR"
     gene            complement(12775..13566)
                     /gene="queC"
                     /locus_tag="PLB01_07265"
     CDS             complement(12775..13566)
                     /gene="queC"
                     /locus_tag="PLB01_07265"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HQQ77138.1"
                     /translation="MGKRAGDGGSRGRAGRRDGRPLHLLRKSSAAIPDPPIAGRRTCV
                     LLLSGGLDSATCLALVSRWGWTVHALSFDYGQRHRVEIDAARALARRYGVASHRVIAI
                     PSFGALGGSALTDRSIRVPKKALGKARIPVTYVPARNTLFLAFALGAAETAGATEIVI
                     GANALDYSGYPDCRPAFLRAFERVANLGTKAGDEGRRFAIRAPLLRLLKARIVRLALA
                     LGLDPGLTTSCYDPAPRGRPCGACDSCLLRAKGFAEAGAADPRAR"
     gene            complement(13585..14241)
                     /locus_tag="PLB01_07270"
     CDS             complement(13585..14241)
                     /locus_tag="PLB01_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ77139.1"
                     /translation="MAAAPLVVTEIFRSLQGESSFAGLPCTFVRLTGCALRCTWCDSA
                     YAFAGGTVMAEGDVLAEVGRLGAPLVEITGGEPLEQEGFYALASALCDRGYTVLVETG
                     GHVPVGRVDPRVIKIVDVKCPGSGMEQRNDLGNLKLLGPRDEVKFVVADREDFEWAER
                     LVAARGLDEKHTVLISPVFEGGASAIAPRVAEWVRDSKRKLRLNLQVHKVLWGDVPGR
                     "
     gene            14274..15059
                     /locus_tag="PLB01_07275"
     CDS             14274..15059
                     /locus_tag="PLB01_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HQQ77140.1"
                     /translation="MRLKLEIAYLGTFFEGWQVQAPRGGGEKPRTVQAELEISLKGVF
                     GGADVRVHGAGRTDSGVHADAQVAHADLPAGAPSIPFDGMRRALNSRLPWDVRVLSAS
                     EASPDFHARYWASGKVYVYRLRRGDILLPHGGLVEALAGEPLDVGAMRAAAERLVGTR
                     DFAPFSITGSDPGTTVRTLRRLDVEEDGSLLVITAEGDGFLRGMVRRLVGTLRDAGRG
                     YIRPAEALERPGPTAEARGLTLARVLYPRDATGAPAPSAPPAR"
     gene            15093..>15732
                     /gene="uppS"
                     /locus_tag="PLB01_07280"
     CDS             15093..>15732
                     /gene="uppS"
                     /locus_tag="PLB01_07280"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQQ77141.1"
                     /translation="MIDKDSIAAAGSPDRALLDGLDLARLPRHVAVIMDGNGRWARQR
                     RLPRVEGHRAGIASVRATVETAARLELTALTLYAFSTENWKRPHLEVATLMALLKEYL
                     RRELKTLLDNDIMFRVVGRMDGLDPSVQRELQSALDATAHCKGMVFNIALNYSGRTEL
                     ADAMRSLAAEVKAGRLQPEEIDEAAIEAHLYTAGLPDPDLLIRTSGEMRISNY"
BASE COUNT         2166 a         5482 c         5567 g         2517 t
ORIGIN      
        1 gcagagccgc gctggcagcg atcctcggaa cgctcgcctg gggaatcacg gacgagctcc
       61 accagtcgta cgtcccgaac cgcacccccg aagccgccga cgtcctggcg gacgtgacgg
      121 gggcggcgct ggcagcggcc gccgcgcgaa tgctgaagaa gtaatcaaac ccacggcggc
      181 gtccccaccc cgggccgcgg agggggtttc ccgcaagtga gccccggccg caggccggat
      241 ctcacgccgc ggcgcccggc gaagccggca aacttggctg gggggcaggg atttgaaccc
      301 cgatagccag atccagagtc tggagtccta ccgttggacg accccccagc gaggcgggcg
      361 gcggattatg agggggcggc gcgccgcggt caaacggcgc gccgctcacg cccttcgttc
      421 gagccccgac gtgcggagaa tccactctcc ggacgcgacc gcgaagccga cgcccgccac
      481 gtccgcgagg acgtccgcga tctcgacgtc gcgcccggga acgaaggact ggcggatttc
      541 gtccgagcag ccccacaggg ccgctccgag gaggagaaag acggccgtct tcgccccgct
      601 ccagccctcg ccgaaacggg cggcgcggac cgcgaggaga cccgtcagca ggaagtaggc
      661 cgtgtgctcg accttgtcct tgcccgtgaa ccagggtccg agcggcggca ggccggacgg
      721 cggccggccc gagagccaga actcgtatgc gagaaggaga acgacgggcg cccagagcag
      781 gagccgtcgc cgcgatctca tcccgctgct gcggccttct tctcgatctt cccccacgag
      841 tcgcggagcg ataccgaccg gttgaagacg aggcggccgg gagcgctgtc cgggtccacg
      901 acgaagtagc ccgtacgctc gagctggaag gccgtgcccg cgggcagcgc cgcgagcgcg
      961 ggctcgacgc gcgccccgct ccgcacgacg agcgagtccg ggttgaggtt cgcgaggaag
     1021 tccttcccct cgggtgcttc gtcgggattg ggcgccgtga agagccggtc gtagagccgc
     1081 gcctcggcgg ggagcgactc ggcggcggag acccagtgga tcgtcccctt gaccttccgt
     1141 cccggggcct cgccgccctt cgtcgcggcg tcgtacgtgc accgcagctc cgtgacggcg
     1201 ccggcggcgt ccttgacgac gtccgtgcag cggatcacgt acgcccagcg cagccggact
     1261 tccgtgccgg gcgagagtcg gtggtacttc gggggaggaa cctcgcggaa gtcgtcgcgg
     1321 tcgatgtaga gcgtcccgga gaacggcacg gcgcgcgtcc cctgcgaggc gtcctcgggg
     1381 ttgttcacgg cgtcgagcat ctcgaccttc ccctcgggcc agttcacgag gacgaccttc
     1441 accgggtcga ggacgcccat gaagcgcggc gcgcgccggt tcaggtcctc gcgcaggcag
     1501 tgctcgagga gctcgacctc gacgacgctg ttgcgcttgg cgaggccgat gcggtcgcag
     1561 aactcgcgga tggcctcggg cgtgtacccg cggcggcgga accccgcgag cgtgggcatg
     1621 cgcgggtcgt cccagcccgt gacgtgcttg ccggtcacga ggtggaggag cttgcgcttg
     1681 gagagcagcg tgtacgtgac gttcagccgc gcgaactcga tctggcgcgg ccggtccggc
     1741 aggtccagag cctcgaggca ccagtcgtag agcggccggt gatcctcgaa ctcgagcgtg
     1801 cagagcgagt gcgtgatgcg ctcgatccag tccgagatcg cgtgggcgta gtcgtacatc
     1861 gggtagaggt gccacgcgtc gcccgtgcgg tggtggaccg tcttacggat ccggtagagc
     1921 gccgggtcgc ggaggttgat gttcggggac gccatgtcga tcttcgcgcg cagggtgcgc
     1981 gcgccgtcgg ggaactcgcc cgcgcgcatc cggcggaaga ggtcgaggtt ctcggcgggc
     2041 gtacggtcgc ggtacgggct gttcgtgccg ggttccgtca acgtcccgcg cgtcgcgcgg
     2101 acctcgtcgg ccgtgaggtc gcacacgaac gccttgccct tgccgatgag cgcctcggcg
     2161 aactcgtaca gcttttcgaa gtagtcggac gcgtagaaga gtcggtcctc ccagtcgaac
     2221 cccatccact tcacgtccgc ctgaatcgag tcgacgtact cgacctcttc cttgagcggg
     2281 ttcgtgtcgt cgaagcggag gttgcaggtc ccgccgtact ccttcgcgat cccgaagttc
     2341 aggcagatcg acttcgcgtg gccgatgtgg aggtacccgt tcggctcggg cgggaaccgg
     2401 gtcgccacgc gcccgccgtg cttgccggag gcggcgtccg cgtcgacgat ctcgcggatg
     2461 aagtccttgc cttgggcggg gggcgcgggc gtcttcgtgt cgtccatgga atccctcagt
     2521 tcggggccgc cggcgcggcg atgcggtcgg cgaacgcgag gatcctccgg cgggccgtct
     2581 cccggccgag aacctcgatc atctcgaaga gcggaggcga cgcgagggtc cccgtgatcg
     2641 cgaggcgcgc gggctgcgcg atctggccga gcccgaggcc cgtctcggcg gcgagcgcgc
     2701 gggccgcctc ctccatcccc gcgtgacgga acggatccac cgcgtcgaga cgctccgcga
     2761 gcgcgcgcag gaccggtgcg agctcgggct tgccgaactt cttcaggccc gccgggtcga
     2821 acacggccgg atcgtcgccg tagacggaaa ggccggcggc gagctcgacg agcgtcttcg
     2881 cccgcggcct gaggagcgcg acgcccttcg cgagcgtctc cggatcccgc gcgggcaggc
     2941 cgaacggcgc gaggagctcg aggaggcgcg cgtccggcgt gtcgaggatg tactgcccgt
     3001 tcatccactg gagcttctcg tggtcgaagc gcgaaggcga ggggccgatg cgctcgagcg
     3061 cgaagagggc cgtcatctcg gcgagcgtca tccgctcgcg gtcctcgccg ggcgaccagc
     3121 cgaggagcgc gaggaagttc acgagcgcct cgggcaggaa gccctggtcc cggaactcct
     3181 cgacgcccgc cgcgccgtgg cgcttgctga gcttcctgcc gtcggcgccg aggatcatcc
     3241 cgaggtgcgc gaagacgggg cagggcgccc cgagcgcgcg gaagagcgcg acgtgcttcg
     3301 gggcgttcgg gagatggtcg acgccccgca cgacgtgcgt gatgcccatc gcgacgtcgt
     3361 cgacgcagac cgtgaagtgg tagagcgggt ggccgtccga gcgcacgagg acgaagtcgt
     3421 cgagcgcgcc cgccggaaac tccgagcggc cctggacgac gtcgtccacg acgagcggct
     3481 cgtcgggaac gcggaggcgc aggacgaact tctcgccctg cgcggcgcgg cggtcggatt
     3541 cttccttcgg gatcgcgcgg gccgcgccgt cgtacttgaa ggacgagccc tcggcctcgg
     3601 cggctttctt ctgcgcgtcc agctcctcgg gcgtctcgaa cgcacggtag gccttgccct
     3661 cggccaggag ccgttcggcg gcctcgcggt agaggtcctg gcgttcgctc tggaagaacg
     3721 ggccctcgtc ccacgagagg ccgagccacg tcatcgcgtc gaggatctgc tgcgtgagct
     3781 ccttccgcga gcgctcgacg tccgtgtcct cgatgcgcag gatgaacgtc ccgccgtggc
     3841 ggcgggcgaa gagccagttg tacagggcgg tgcgcgcgcc gcccacgtgg agagagcccg
     3901 tgggcgatgg ggcgaaccgc acgcggggcg cggtcatggg tcggcgagga taccagcccg
     3961 cgcctcgggc atgatgccgt ccgatgagcg ccggcccccg ttccgtcccg cgccgcgagg
     4021 ccctcacggc ggacctcgtc ctcgtcgtcg tgacggcgat ctggggctcc acgttcatcg
     4081 tgaaccgcgt cgtcatccag tcggcgccgc ccctcctctt cgtcctcgcg cggttcgcgc
     4141 tcgccgcggc gatcctgtgg ctgatcgcgc gggggcgccc gcggacgccc aatctcgtcc
     4201 gggacagcgc ggcggtcgga ctcctgctcg cggcagggat cgggttccag gtcgtcggcc
     4261 agctcttcac gacggcctcg aaggccgcgt tcgtcacggg tctctccgtg cccctcacgc
     4321 ccgtcgcggg ctacctcatg acgcggaaga agccctcgcg cgagaacctc gccgggctcg
     4381 tgatcgcggc gctcgggttc gcggtcctca cgtggccgaa ggacgcgtcg gccgggttca
     4441 acacgggcga cctcctcatc ctcgtgacgg ccgtgtcgta cgcggtcctc atcgtgatcg
     4501 tgagcgagac cgccggaacg cacgacgtga ggtggtactc cttcgggcag atcgtcttcg
     4561 cggcgatcgg cgtcggcgcc gtgcgcctcg cgctcacccc gttcgtccac gggtccggcg
     4621 cgttcctcgc ggccgaggcg cggccgctcg tcctcggccg gacggtcgtc ctcgcgatcc
     4681 tctggatggc gctcgtcgcg accgtcgtga cgttcctcgc gcagacgtgg gcgcaggcgc
     4741 gcatgcccgc gacgcacgcg gcgatcatgt tcgcgctcga gcccgtgttc acggccctct
     4801 tcgcggcgtt cttcctcggc gagcgcatgt cgcgccgcga ctgggcgggg gccggcctcg
     4861 tgctcgccgg gatcctcgtt tcggagctgc gcgggcggga ctgagctact ccaacccgaa
     4921 cgtcatctgg tagacgtacg gctcgcgcag gacggacagg acgaggctcg ggcgcgccca
     4981 gccgctctcg acggccctgc tgaggtcgtc gagcgtccgc accttgacgt tgttgacggc
     5041 gaggacgacg tcgccgcggt cgaggccctt ggcggcggcc ggagagcggc tcaagacgga
     5101 cgcgatcatg aggccggcgc gcgtctcctt gaagacgagg ccgagctccc gccggagaac
     5161 gtccagcccg aggtcctccg gcgtgcgctc cgtcgtgacg cgggccgtgc ggtctgcgcc
     5221 ctcgcgccga tacacgaccg cgagcgtctt gccgggcccg accgacgcga gcgtcgtgtc
     5281 gaagtcctcg cggccctcga cgggccgtcc gtccagcgac acgacgacgt cgccgggcgc
     5341 gaggcccgcc ttcgcggcgg gcgagcccgg ccagaccgaa cgcacgcgca ggccctggcc
     5401 ccgggccgac ggccgtccgt tcgcgggcgg gagcgtcgtc ccgcggacgc cgatccaggc
     5461 ggtcttgacc tcccggtagt tcaggaggtc gtccgcgacg cgccgcacgc ggtcgatcgg
     5521 gatcgcgaag ccgatcgtgt tcgcgccgcc gacgatcgcc gtgttgatcc cgatgagctc
     5581 gccgaggatg ttcacgagcg cgccgcccga gttgccgggg ttgatcgcag cgtcggtctg
     5641 gaggaagtcg gagtagttgc ggcccgactc gcccttcacc gtgcggtgta cggccgagac
     5701 gatgcccgtc gtgaccgtgt tcgtgagccc gaacgggttt ccgatcgcga tgacggtctc
     5761 gccgatcatg aggtccgacg tcgtgccgag cgccgcgggc ttcagcccct tcgggtcgac
     5821 gctcaggacc gcgaggtcgt tgtccgcgtc ggcgccctcg agcttcgcct ccagctcgcg
     5881 gccgtccgcc gtcgtgaccc agatgcgcga cgcgcccgag atcacgtggt cgttcgtcac
     5941 gacgacgccg ggcgcgatca cgacgcccga gccgagcgac tcgctgcgcg acgtgcgcgg
     6001 gcggctctcg cccccgaaga agaggtcgac cggactgcgc cgctggacga cgcgctcggc
     6061 cgagatgttc acgaccgacg ggctgatctt ctcgatggcg gcgacgatcg gggtccggcg
     6121 ctcctcgcgc cccgcggccg agaggggcag cgaggcggcg agcgcggcga gcgcgaatgc
     6181 gagacgggtt gacttcattc taaggagttc cataattaag tcgatatacc ggaaaggaat
     6241 tttaagtatt ctaaacaccc ggcagaagaa agcggtccag gcgctcgtcg agctggtcta
     6301 tcgcgcgggc cccgacgtgc cgtcgggcca gctcgcaacg tggctggacc tctcgcgcga
     6361 ctcggtccac cagctgctcc tgcccctggt gaggaacgga tgggtggcgg cgggccgcgg
     6421 gcgcaacggc ggctacaggg cgacgccggt tgcgccgggc gtgaccgtcc tctccgtcgt
     6481 gtcgctctac tcgcgcgagg gccgcgatcc cacgcccgac gacccggcgg cgccgaggtt
     6541 catccgcaac ctcgaccagc gggcatccga acgctatcgg caatatcttt cctccgtgac
     6601 ggtggggggc ctcgtcgccg aggtccgggc cgagcgcgag gagcccacct actcgatctg
     6661 accctctcga cccgactcta gcaaggcgtg cgcccgccgt tccggcgcgg cgacagagga
     6721 aagcacatga agatcgcatc ggacatcacg aaactcgtcg gcggaacgcc gctcgtccgc
     6781 ctgaaccgcg tcacggcggg catcgcctcc ggcgcgacgg tcgccgcgaa gctcgagttc
     6841 ttcaacccgg cccactccgt gaaggaccgc atcggcgtcg cgatgatcga ggcgctcgag
     6901 aagcagggaa agatcgcgcc gggcaagtcc gtcctcatcg agccgacgtc cggcaacacg
     6961 ggcatcggcc tcgcgttcgt cgccgccgcc aagggctatc gcctcatcct cacgatgccc
     7021 gagacgatgt cgatcgagcg tcgcaaggtc ctgaagctcc tcggggcgga ggtcatcctg
     7081 acgcccggcc cgctcgggat gaagggcgcg atcgcgaagg cgcaggagct gctcgccgag
     7141 tacggcgtca acggcgtcca gcctcagcag ttcgagaacc ccgcgaaccc ggcgatccac
     7201 gaggccacga cggccgtcga gctctggaac gacacggacg ggaagatgga catcttcgtc
     7261 gccggcgtcg gcacgggcgg cacgatgaca ggcgtcggcc gctactggaa gccgaagaag
     7321 ccggccgttc gcttcgtcgc ggtcgagccc gtgcactcgc ccgtcatctc gggcggcgcg
     7381 ccggggccgc acaagatcca gggcatcggc gcgggcttca tcccgaagaa cctcgacacg
     7441 acgttcgtgg acgagacgtt ccaggtgtcg aacgaccagt ccttcgcgat ggcgcggcgc
     7501 ctcgcgaagg aggagggaat cctcggcggc atttcgtcgg gcgccgccgt ccacgccgcg
     7561 gtcgagatcg cgaagcggcc cgagagcgcc gggaagctga tcgtgtgcgt cgtcccgtcg
     7621 acgtccgagc gctacatctc gacggatctc ttcaaggacg acgaagtccc tgcgggagcc
     7681 gcggcgatct agtggatctg cgcgggggaa cccctcccgg gctcccccgc gtcgccctcc
     7741 cggtcaggat ttcttggcct cgatcgtcgg cttcgccgcc tggatcttga tggctttggg
     7801 cttcgtctcc tcgcgcttgg gcagctcgac ggtgaggacg ccgttgtgga acgaggcgac
     7861 gatcttctcg ttgtcgacgt tcgcggggat cgtgaacgag cggacgaact tcccgtagct
     7921 gcgctcgatg cggtggtagt tctcgtcgtc gctcgccttc tcgaacttgc gctcgccttc
     7981 gagcgagagg atcccgtcct cgaagcgcag cgcgatctgg ttctcgtcga agccgggcag
     8041 ctcggccttg aggacggtct tctccttcgt ctcgaagatg tccacgggcg gcgcccaggt
     8101 cccggtgaag agctcttcct gctgctcgcg cccgccgcgg gcgagcgtgg agtcgaacgc
     8161 gcggttcaga cggtcctgga gcgtagcgag gtcgcggaag gggtcgttcc agcggatgac
     8221 gggcatgggt ctcgttcctc cttgatcaac gtttcttcgc cggcgcttcg gccggctcgg
     8281 tccggaactc gagggcggag ccgcccgggg cgagcgcgag cgtgaccgcg gatccgtcgc
     8341 gcacctcgcc cgcaaggagc ttccacgcca gcgggtcctg gaccagtttc tggatgtttc
     8401 gcttgagcgg ccgcgccccg tacacggggt cgaacgcctc gcgcgcgagc cacgtggcga
     8461 ccggcggctc ccacgcgagc ttgatcccgc gcggcgcgac gagctcggcc accttgcgga
     8521 gctggatctc gacgatcttc tcgatgtcgg agagctcgag gcggcggaac gtcacgatgt
     8581 cgtcgatgcg gttcaggaac tcgggccgga aatgcgccct gagctcggtc tcgatgcgcg
     8641 ccttgagcgc tttttcgtcg acgccctgca gctcctgaat gaggtgcgag ccgacgttcg
     8701 acgtcatgac gacgacgacg ttcttgaagt ccacgacgcg gcccttaccg tccgtcaggc
     8761 gcccgtcgtc gagcacctgg aggagcgtcg accacacgtc cgggtgggcc ttctcgatct
     8821 cgtcgaagag gacgacggag tagggacgcc ggcgcacggc ctccgtgagc tggccgccct
     8881 cgtcgtggcc gatgtagccc ggaggggccc cgatcatccg cgagacggtg tgcttctcct
     8941 ggtactcgga catgtcgagg cgcaccatcg cgttttcgtc gtcgaacagg aactcggcga
     9001 gcgcgcgcgc gagctccgtc ttgccgacgc cggtcggccc gaggaagagg aacgagccga
     9061 tcggccggcg cgggtccttc atcccggcgc gggcccggcg cacggcggcc gcgacggcgt
     9121 cgaccgcctc gtcctgcccg atgacgcgca ggcgcagccg gtcgcccatc ttgaggagct
     9181 tctgggtctc gccttccacc atccgcgaga cgggcacgcc cgtccacttc gagacgatgg
     9241 ccgcgacgtc gtcctcgtcg accttctcgc gcaggatcgc gccgcccttc tgcatctcgt
     9301 cgaggcgcgc ctgcgcggcc tcgaggtcct tctggacctt gaggagcgtc ccgtagcgca
     9361 gctcggcggc cttgttcagg tcgccctgac gctcggcagc ctccgccgcg accttcgtct
     9421 cgtcgagctt cttcttgagg tcgcggatca cggtgatcgc gtccttctcg gcgcgccacc
     9481 gcgtcttcat ggcgttggag cgctccttga gctcggcgag cgtcttctcg aggcccttga
     9541 gccgctcctt ggcggccttc gcgtcgtcct tctggagcgc cttgcgctcg atctcgatct
     9601 ggaggatgcg ccgctcgacc tcgtcgatcg cggtcggcat cgagtcgatc tccatcttga
     9661 gcttgctcgc cgcctcgtcg atgaggtcga tcgccttgtc ggggaggaag cggtccgcga
     9721 tgtagcggtc cgagagcgtc gcggcggcga cgagggccgc gtcggtgatc ttgacgccgt
     9781 ggtggagctc gtagcgctcc tgcaggcccc gcaggatcgc cacggtgtcc tcgacggagg
     9841 gctcgcccac gaggacgggc tggaagcgcc gctcgagcgc cgggtccttc tcgatgtgct
     9901 tgcggtactc gccgaccgtc gtcgcgccga tcgcgcgcag ctcgccgcgc gcgagggcgg
     9961 gcttgagcat gtttcccgcg tccatcgcgc cctcggcggc gcccgcgccg acgagcgtgt
    10021 ggagctcgtc gatgaagagg atgaccttgc cgtcggattc ctcgacttcc ttgaggaccg
    10081 ccttgaggcg ctcctcgaac tcgccgcggt acttcgcgcc cgcgagcatg aagccgaggt
    10141 cgagcgagac gacgcgcttg tccttgagcg tctcgggcac gtcgcccgag acgatgcggc
    10201 gcgcgagtcc ctcgacgatc gccgtcttgc cgacgccggg gtcgccgatg aggacggggt
    10261 tgttcttcgt gcggcgggtc aggacctgca tgacgcgacg gatctcctcg tcgcggccga
    10321 tgacggggtc gagcttgccc ttgcgggcga gctccgtgag gtccttcgcg tatttcttga
    10381 gcgcctggta tttgtcctcg gcaccctggt ccgcaacccg gctcccgccg cggacgtcct
    10441 tgagagccgc caggacggag gcctccgaga ggccgagggc gaggaaggcc ttcgcggacg
    10501 cggacctgcc gtccttgagg agcgcgagga agaggtgctc ggacgcgacg tagtcgtcct
    10561 tgaaatccga ggcgatcttc tccgcgaggt cgagcagctt cacgagtgcg ggggagaagc
    10621 gcggttccgc gttttcgccc gcgacatggg ggagcttcgc gagcagcttc gcgacctcct
    10681 gcgcggcgtg gccgggggcg acgccgacgc gttcgaggag cgccggcacc acgccccctt
    10741 cctgttcgag gagagccgcg gcaaggtgtt cgggcgtgac ctccggatgg ccgtgagccc
    10801 gcgcggactg tgccgtcttc tgaaggactt cctgggcctt gtgggtgaag cggtcgaggt
    10861 tcatgacgtc gccgaatcta gtatctgagt gcttggttgt caagtgtgaa tctacagtgt
    10921 gaatgaagat aatattaagt cgtttcttct gaatggtatg ggagccgatt cggcccatgc
    10981 taggctttca cggcgatggg gcgccgaggc gctgggaggc gcgaagatgg ccgcggctgg
    11041 ccacgtttca taggaatctt cctcttcgct ttcggttttc gtttcttcgt ctcctcgtcg
    11101 ctctcgcacc tcggcctctg gcagagcccg cagttcgatg cgcacgagaa cctcgcttgg
    11161 gcgcacgctc tcgccgccgg cgaattccag tggccctccc cgccgacgca cggcccggcg
    11221 tatcccttct tgctcgcgct tctcctgaag gtcttcggca cggtgagcgc ggcgcgggtc
    11281 gcgcaagccg cgctcgcgtc cgcgacgtgc gttctcgccg cgaggacggg agagcttctc
    11341 ttcgaaaggc gggccggcct cgcggcggga gtcctgctcg cggtctccgg tccggtcgcg
    11401 ctcgtggacg tcagcttctg ggaagagggc ttcgtctcgt tcctgctctc ggcggcgctc
    11461 ctcctgctcg tggcgcggag gacacccgcc ttcgccggcg cgtccggcct ccttctcggc
    11521 atcgcgtgcg cggcgcggcc gacggcggtg ctgttcgttc tcgcggcgct cgccttcgtt
    11581 ctcttcctcg aaggctcgtc tcgaccgggg ccgacggcgg cggcgctcct tcttggcgtc
    11641 gccgtcgtgc tcctccccgc cgtcatcggc tcgtcgcggg ccgccggcca tttcgtcttc
    11701 gtccgcgcct acggcgccgt gaacctctgg atcgggaacg atccggcggg aggcggcgtt
    11761 cagaacgcgc ggccaaacgg gccgtgggac cggctcgcag cggaacccgc ccgcgcgggc
    11821 gtcgctccga aggacgagga ggcgtggttc acgcgccgcg cgatcgagcg cgccaccgcg
    11881 gatccggtag gcctcgcccg cgtggtcctt tcgaagctcg cctggctgac gcaggccgag
    11941 gagccgcgcg acaaccacgc gttcgcgttc tttgcgtcgc aatcggggat tcttcgaata
    12001 ctccccggct tcgggttgct tttcgccatg gccgctgccg cgttcttcgc gcgcctcccg
    12061 ctgcggaccg cggggctgcc gctgctttgg atcgtggcgg gcgtccttcc gttcctcgcg
    12121 gcgctcgcgg ggctccggta ccggatgccg gtggtgcccg ccgtcgctct cttcgcgggc
    12181 gccggcgcgg ctcacctcct tgatcgcgtc aagaagcggg acctgcgcct ggtcgcggcg
    12241 cccgcggtgg tcgtcgcgtt gacactctgt gcttcccacg ttcgaaggca tcctccgtcg
    12301 cacgtcttcg cagaggaatg ggcgctggag ggcaacgcgt ggatggagct cggaaaaccc
    12361 gccgaggccg agcgcacgct gcgccgcgcg gtcgaggcgg acccgaaggc ggctcttccg
    12421 gtggagttcc tcgggcggct ccggctcgca cagggacgtg ccgccgaagc ggcggacacc
    12481 ttcgaagaat ctcttcgaat ggatcccgat tcgcgctcgg ctcacttctt cctcggacag
    12541 gcgcgcgaag cgctgggcct ctggcccggc gccgcttccg cgtaccgcgc cgctctcgcg
    12601 atctcgccgg cgttcttccc cgcgcagttc cacctcgggc gcgcgctgct tctggccggc
    12661 gacccggcgg gcgcggtggc cgcgctcgag aaggcggtcg cccttcagcc tctcgaacag
    12721 ccgccgcgcg aactcctcga gaaggcgcgc gctctcgccc tctctccgcg ctgatcagcg
    12781 cgcgcgcgga tccgctgcac ccgcctcggc gaagcctttc gcacgcagca ggcacgagtc
    12841 gcacgcgccg cagggccttc cccggggcgc agggtcgtaa caggacgtcg tgaggcccgg
    12901 gtcgaggccg agcgcgagcg caaggcggac gatgcgcgcc ttcaagagcc gcaggagagg
    12961 cgcgcggatc gcgaaccggc ggccctcgtc gcccgctttc gtcccgaggt tcgcgacgcg
    13021 ttcgaacgcg cggagaaacg cgggacggca gtccgggtag ccggaatagt cgagcgcgtt
    13081 ggcgccgatg acgatctccg tggcgccggc cgtctccgcc gcgccgagcg caaacgcgag
    13141 gaagagcgtg ttgcgggccg ggacgtacgt gacagggatc cgggctttcc cgagcgcctt
    13201 cttcgggacg cggatcgagc ggtccgtaag cgcggagccg ccgagcgcac cgaacgaggg
    13261 gatcgcgatc acccgatgcg acgcgacgcc gtagcggcgc gcgagggcgc gcgcggcgtc
    13321 gatctcgacg cggtggcgct ggccgtagtc gaaggagagc gcgtggacgg tccagcccca
    13381 gcgcgagacg agcgcgaggc acgtcgcgga atcgagcccg cccgagagga gaaggacgca
    13441 ggtccttcgc cccgcgatcg gtggatcggg tatcgccgct gaagattttc ttagaaggtg
    13501 aagagggcgg ccgtcgcgcc gccccgcccg accgcgcgag ccgccgtccc ccgccctctt
    13561 acccattcga agaaatctct ctcttcatct tcctggaaca tctccccaga gcaccttgtg
    13621 cacctggaga ttcagccgca gcttcctctt cgaatcgcgc acccactcgg cgacgcgcgg
    13681 ggcgatcgcg ctcgcgccac cctcgaagac gggcgagatc aggaccgtgt gtttctcgtc
    13741 gagccctcgc gccgcgacga gtcgctcggc ccattcgaag tcttcccggt ccgccacgac
    13801 gaacttcacc tcgtcccgcg ggccgaggag cttcaggttg ccgaggtcgt ttctttgctc
    13861 catgcccgag ccggggcatt tgacgtccac gatcttgatc acgcgcgggt cgacccggcc
    13921 cacggggacg tgcccgccgg tctcgacgag gacggtgtat ccccggtcgc acagcgcgga
    13981 cgcgagcgcg tagaagccct cctgctcgag cggctccccg cccgtgatct cgacgagggg
    14041 cgccccgagg cggccgacct cggcgaggac gtcgccttcc gccatcaccg ttccgcccgc
    14101 gaacgcgtac gcgctgtcgc accacgtgca gcgcagcgca cagccggtca gccgcacgaa
    14161 cgtgcacggc aggcccgcga aggacgactc gccctgcagg ctgcggaaga tctccgtgac
    14221 gacgagggga gcggcggcca tcgcgcggac gatagcgcgg ccataatcct cgcgtgcggc
    14281 tgaaactcga gatcgcctac ctggggacct tcttcgaggg ctggcaggtc caggcgcccc
    14341 gcgggggcgg cgaaaagccg cgcacggtcc aggccgaatt ggagatttct ctgaaagggg
    14401 tattcggggg agcggacgtc cgcgtgcacg gggcggggcg caccgactcc ggtgtccacg
    14461 ccgacgcaca ggtcgcgcac gcggaccttc ccgccggcgc gccctctatt ccattcgatg
    14521 gaatgcgccg tgccctgaat tccaggcttc catgggacgt gcgggtcctg tcggcgtccg
    14581 aagcctcgcc ggacttccac gcccgctact gggccagcgg caaggtctac gtctaccgcc
    14641 tccgccgcgg cgacatcctc ctcccgcacg gaggcctcgt cgaggcgctc gcgggagagc
    14701 cgctcgacgt cggcgcaatg cgagcggcgg cggagcggct cgtcgggact cgcgacttcg
    14761 cgccgttctc gatcacgggc tcggacccgg ggacgaccgt gcggacgctc cggcgtctcg
    14821 acgtcgagga ggacggctcg ctcctcgtga tcaccgcgga gggcgacggc ttcctgcgcg
    14881 ggatggtgcg gcggctcgtg gggacccttc gcgacgcggg gcgggggtac atccgtcccg
    14941 ccgaggccct cgagcggccc gggcccacgg ccgaggcccg cgggctcacg ctcgcccgcg
    15001 tgctctatcc ccgggacgcg acgggggcgc cggctccgtc cgcgccgccc gcgcgataga
    15061 attccacgtt cgggcgcatc ccgcccggcc ggatgatcga caaggattcc atcgctgccg
    15121 ccggttcccc cgaccgagcc ctgctcgacg ggctcgacct cgcacggctg ccccggcacg
    15181 tggccgtcat catggacggc aacgggcgct gggcgcgcca gcgccggctg ccgcgcgtgg
    15241 agggccaccg cgccgggatc gcttccgtgc gtgccaccgt cgagacggcc gcgcggctgg
    15301 agctcaccgc gctgacgctc tacgcgttct ccacggagaa ctggaagcgg ccgcacctcg
    15361 aggtcgcgac gctcatggcg ctcctcaagg agtacctcag gcgcgagctg aagacgctgc
    15421 tcgacaacga catcatgttc cgcgtcgtcg gccgcatgga cggcctcgac ccttccgtcc
    15481 agcgcgagct gcagagcgcg ctggacgcga cggcgcactg caagggcatg gtcttcaaca
    15541 tcgcgctcaa ctactcgggg cgcaccgagc tcgccgacgc gatgcggtcg ctcgcggccg
    15601 aggtgaaggc cggacgcctg cagcccgagg agatcgacga ggccgccatc gaggcgcacc
    15661 tctacacggc cggtctgccc gacccggacc tcctgatccg gacgagcggc gagatgcgga
    15721 tctcgaacta cc
//
LOCUS       DAOMFY010000063        15664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1160_length_15664_cov_6.765264, whole genome shotgun sequence.
ACCESSION   DAOMFY010000063 DAOMFY010000000
VERSION     DAOMFY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15664
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_15664_cov_6.765264"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLB01_07285"
     CDS             complement(<1..352)
                     /locus_tag="PLB01_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77142.1"
                     /translation="MEGTVRRVHALTAALSKRRAALETLLAGGAGAPTGERLHAVRVA
                     LRRTVSLARLTQDVPAKGSGAGLKTAARDLRRALGVRRTHDVAAKLLRARFRRDARRR
                     ASAERLAERLERDAR"
     gene            complement(354..1694)
                     /locus_tag="PLB01_07290"
     CDS             complement(354..1694)
                     /locus_tag="PLB01_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ77143.1"
                     /translation="MSANPLPAPAAPIASARGVDKIFEDEGGRARVILKAIDFEVRPN
                     EVVAVLGPSGCGKSTLLRILIGLIPPSAGAVEQHGKPLVGIHPGAAVVFQNFALFPWL
                     TVEENVRVGVAGRATDARDADERVKTALSAVGLAGHARAFPKELSGGMKQRVGIARAL
                     VGRPELLCMDEPFSALDVLTAELLRTEVYRLFTERLTGLSSVLLITHLIEEAVFLGDR
                     IVVLGTNPGTVRREIVNTLPHPREYRHPEFLRMVERIHDVVTGVHLPEEEEAAARIPG
                     RRVETPLIPLPPARMGEILGLLEILADNGGTMNIFAVDALTEYDFGRTLTVVKAAELL
                     DLVDTPKNDVLLTDLGRRVVAAPLDAKIPLLRPQILSLGTFCLLVRELARDPDTPVPG
                     ETLRDTLAASLPTVSASELFETMVNWGRAIQVFEYDANTDQLALFSGRVAAAEG"
     gene            complement(1691..3364)
                     /locus_tag="PLB01_07295"
     CDS             complement(1691..3364)
                     /locus_tag="PLB01_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ77144.1"
                     /translation="MDVLILIAVGALVAGIVALAQRWEAPIRPTVEIDLSPSALPGYT
                     LLSLSRGFAAYLLSLVFTLVYGTIAAHGKRAERVLIPLLDIGQGIPVLGFLPGLVLGM
                     VALFPRTNVGLELACIVMIFTGQVWNMTFSYIASLRSIPNELNEVARLNRFSFWKRFR
                     TVEVSSSVIGLVWNSMMSMAGGWFFLTVNEAFTLGDRDFRLPGVGSYMAVAIEKGDKA
                     AMAWAVLAMVVMIVAVDQLLWRPLVAWSQRFRAAEVSGASEARSWVLDLLRRSAVLRR
                     MRLRRRRRPKAPAAPLARPPESVPGIADAGARLRAALLFVLGVGAAAAALLGAWKLVR
                     LLSAVTPHDWLLLVAALGATFLRTLGALALAVAWTLPVGILVGRSPAWSRRLQPIVQI
                     VASFPAPMLFPIVTGALLALHVPFAVIAAVLMFLGAQWYVLFNVLAGASAVPPDLVEA
                     ADVFGVAGGARWRTLFLPAVFPYLVTGLVTAAGGAWNASIVAETLVSGGRTLETFGLG
                     SLITKATREANFPLLAAGVLTMSFALVLINRTVWRRLSRLAETKYALNR"
     gene            complement(3409..5187)
                     /locus_tag="PLB01_07300"
     CDS             complement(3409..5187)
                     /locus_tag="PLB01_07300"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HQQ77145.1"
                     /translation="MQKPKSQTDTQPIKLREGRKASGVIRRPATLLVDSPDATVLLDR
                     DGQVLLANVVALAEGVPSPGSVDGPPESAGPFWRDPEGRTRFLEAAASGSVKNFEVRL
                     PAGIDVGERVYWISTCFAPQAGEGGFIASARDVTGAAAGTRGDAAETVAEPADALTGF
                     ANREQFHFELEREIARADLFARPLALIILGLDNFKTLNDTHGLLAGDEYLRAMANTLR
                     PSLLPGEMCGRVGGDEVAMLMPGTGASEATVRAEAVVSIIRKLTPDWEGNALSLTASA
                     GVAVFPDHAGGAADLFLAADLAMHQAKRRGGARVRLHDPSDRERDRTGRLREQSNRIR
                     AALAESRFVPYFQPVAEVGTGRIVAVETLVRMREPDGRIALPGEFLGAAERFGIITAI
                     DRAVIAAAFDALATARKRISPELEIGINLSGLDFDDDTLVADISRLARAKGIRPERVT
                     FEITETAALRDFDRVRDFTHALTSEGFHFALDDFGMGFSSFRYLRELPVSSLKLDISY
                     VRDLPREKANRVFVRGMAGICRGLGVKTVAEGVESEDVMEILPELGVDRAQGWHIGFP
                     EPELPMADPLGETGGRSLTLTRRPRS"
     gene            complement(5223..5708)
                     /locus_tag="PLB01_07305"
     CDS             complement(5223..5708)
                     /locus_tag="PLB01_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ77146.1"
                     /translation="MNAPAFILREALREDVPAIVGLIQDLALYERQPEDCHADPALLE
                     KHLFGPRPYAEAIVAETGGELIGFALFFHNYSTWLTRPGLYLEDLYVKPERRGLGAGK
                     ALLERLARIAVERGCGRMEWSVLTWNEPAIGFYKALGAVSMDGWRTYRLTGDALEALG
                     R"
     gene            5764..6348
                     /locus_tag="PLB01_07310"
     CDS             5764..6348
                     /locus_tag="PLB01_07310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ77147.1"
                     /translation="MAASGSFYGLLTRRVGEARLAAALPHLPRGGGGRVLDVGCGLTD
                     LAARIPDYTGADRNPDVLAEQRGRFPKRDFYEWDIVRGAAPAALSAKAPFGGILLLAV
                     LEHVQEPASVLGRLAPLLAPGGRLIATTPHPSGRWPLEAGAALGLLSSHAHDEHETLL
                     GRESLESAGRASGLSLVLYRRFLLGMNQLAVYTR"
     gene            6345..9416
                     /locus_tag="PLB01_07315"
     CDS             6345..9416
                     /locus_tag="PLB01_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQQ77148.1"
                     /translation="MTAGPRLVRRGADAIHVVNARTHNLRDVTCTIPVGALTVVTGPS
                     GSGKSSLAFDTLYAEGQRRFVESMSTYARQYLERLERPDVDEITHVLPAIAIEQRAPA
                     RSARSTIGSATEIHDVLRLLFSAAGTLRCPDDGSPVRRETAESVRAALLEEFGEGARL
                     LVVAPRRVTSFAAESAEWRRLGFYRYVGAAGDVLEIEEKISSSERERESSSPPRRAVA
                     RSSAARRPSSPFKEIPSSSLFPLLIGRHVLRADDPEILSSLSTAFDAADGSLLVLKSG
                     EGLSSARTFRRGLSCNACGRAFADPVPALFSFNSPRGACETCQGFGRIVGIDAARVIP
                     DPRKTLRERPIAPFNSPSYESAYDDLKAASRRLKLRWDVPWNELTQRERDAVWTRSGD
                     WYGVEGLFEYLEKKRYKVQVRVMLARYRGYSTCPECRGARLRPEALAVTVGGKTIAEV
                     CDLTLDGFVAFLGALPLREAERERAASLVEELARRAGTLVEIGLGYLTLARTMRTLSG
                     GEAQRVQLGSAIGNALTGTLYVLDEPTVGLHPRDTRRLLAVLERLAAAGNAVVAVEHD
                     TDVIRAADHVIDLGPGAGALGGRLVFEGTPEELTEADTATGRSLKKEKEILLASDAAG
                     YGPTVATDAVAAETVGAFGTFFEGGVSAGAPERPEGRIRPAAHSPSKENHRDDSIRVP
                     LESIQVVGARANNLQNLSVSFPLRRLVAVSGVSGSGKSSLVVDVLAAGARQRMGKGLL
                     PGIDAVGEHDGIFGLEHVSDVILVDQSPPGRSSRSNPATVTKAWDEIRTLLARTPAAK
                     ARGLEKGAFSFNAAGGRCERCEGAGVVTVDMQFLADVTIVCDVCDGRRFKPEVLEVRL
                     RGRNVDELLATTVDEARLLFADAPAIASRLQPLADAGLGYLTLGQSTATLSGGEAQRL
                     KIATFLRGAEAPKAPVLFVFDEPTTGLAPSDVDVLLAVFRRLLAAGHSIVAVEHNTAF
                     LARADHLIELGPDGGPDGGRVVFEGEPAALAARGGTPTARALKGRVQFPPR"
     gene            9413..10603
                     /locus_tag="PLB01_07320"
     CDS             9413..10603
                     /locus_tag="PLB01_07320"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQQ77149.1"
                     /translation="MTEEKWRYVLSAEGREIELSDGEVTLGRSRTSTVRLEHESVSRS
                     HALLTFDRGEAVVKDLASSNGTFVGGKRIARETRLVNGDRLQLGAAVIEVRLLAPDAA
                     SERTALMAPDQLPDALPPRPPSGITYEPVPGLTNPGLGPATPLGPDPISASDLFRAVD
                     RPPVAEPSLVVDVFPPAGGAPDEVSTIAPPAIAPSAVAPPAVAPPPRELADYSIRVHE
                     AEKAARLPLGARAAPFRDRRAPSRNETARSVANPFARLLAALVDAVILAAINVLLFAP
                     VFLIDYFRSELQTHDAAPDWAFRAITGLSILLALLANVLYVVGGWAWRGRTPGKSLLG
                     LAVVKRGAPSGQGIGWPSAFVRAIVCLLAGLPLGLGWAWAFFEKERRAFQDLAAGTWV
                     IRVR"
     gene            complement(10590..11426)
                     /locus_tag="PLB01_07325"
     CDS             complement(10590..11426)
                     /locus_tag="PLB01_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77150.1"
                     /translation="MLPRECPVAVPTPRVSGRIPALAGLFLALAVPAAPQSILPPDAA
                     VKAYQAGVAAAARKDWPEVLKRMDEALGTGHRDVKVHFGTNRYYVDLYDPYYWRGAAR
                     MEIGDDAGAREDLARSRDAGEIRRFPEYGDLLARVDTLDRRAAAALEAAATPTPVPPV
                     PTPAPTSAPPLLAPTPRPTRADAGGATIERVLVLFSAGDFEGAEAALAGLRAARPDAR
                     EADLLQCLVLGTRYVLEGESDPILLTRARRSLGAWRERGGPRKVEEALLSPALLAALN
                     GP"
     gene            11449..14505
                     /locus_tag="PLB01_07330"
     CDS             11449..14505
                     /locus_tag="PLB01_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77151.1"
                     /translation="MGFPTRFTSGLAIAMLLAAVFGVRQAGAQTTPYPCPAAAPVLTG
                     PALAQAGETYAVSWTHVLGNFVSSNAAYSYTILRAADKDFTKVTDSITTQRSALTLPA
                     PPAGAATLYHQVVVSTPCTTLGASFSNVLAVPVKTTCDVPPSVGALTASPENPPAFST
                     WVVSWSTLGTGPGPGVGVANLRFRLRRTSALEPEGREWVVDGGSASFAGPPGEYVFEV
                     RAEAACGSVGPWSPPKRVTVGTVAKPTLVLVSEPEPLARIVPAAGVRPGTSFTVRNGG
                     TEPVTATTKCADAGFAITPATFTLPAGAAQEISVTSLYVTALAQPVKTAVTVSSGAST
                     LSVPITIVLAAAAAAAPVRWSQPTADVDVNGTAVTRSVVNGGATTAAFVASVKAPWIT
                     VQSVDGQAWDRPLAPFETRSLRLVFDRAKRRGETGTEVGAVALTTVGFTAPETLVVTD
                     DGPRIPPAGTGAGAIPVASAKTRVLYASFPNALDARGVGRFAADLWITNTDAVNPITL
                     SLLFSPVGAPADGSALKRFDVQLAAGETRRYRNVVATLMGSEGAYTVEIRSSAPTVSA
                     TALVANTALPAVAAARRAQTGLASGSYGFEMRPTSPGEGAKQNDPIQVTSGLAHDANR
                     RSNLLLLETSGYDTTVLVELFNNQGKPVTKNGQPVQLQKTVPASGTLQINDADELFDA
                     APLAGTYAYARITWKSSATDGTGAQKGSVVGMATVIDNRTQDSSLHVGVSTNALDPAY
                     VPTSSLETSRTALVSLPFGGAPAPLLFPTVHSAGAALATGAKPFWRTRVTLTNTSEAQ
                     RSVTLEYLDAVDPSVTRQFGVGLSPGGIFSFEDVLEERLGILPGQNAYGAVKISAAKN
                     NDETWAGVDVQTETYTVDPVQGAGDYKTGMEGYAYFHGYSSFQSNLGTMSFDGAENST
                     GYRTNIILHEVGNAYCDVIISAYLPGSFVPVASKTHRLAPFGYFSDELFTNVLGLNLT
                     EVTDVRVVVRQIAGDGVFLAFASKIDRASGDPANVFLRPAAAGTGR"
     gene            14513..15538
                     /locus_tag="PLB01_07335"
     CDS             14513..15538
                     /locus_tag="PLB01_07335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ77152.1"
                     /translation="MAGAVERIAHYRILKRIGRGGMGIVYQAVDERDGRLVALKVIRE
                     AEAPGDESAVHQHEARVRFDREAAILKSLLHVNVVTFYEIGEEDGTPWLAMEYLDGVP
                     LTSYAGKPWTETLPLLVQAAHGMEYLASRGIVHRDLSPDNILVIERGGDLIVKLLDFG
                     IAKLFEHATGLESLTATGFFLGKVAYGSPEQLGSLGHGATLDWRSDVYSLGVIFYQVL
                     AGQRPFDGKAPVEYIAAHLNATAPPVAAPHDAPALPVPLVRLIGQMLEKRREDRPGGW
                     REIVDRLVGVLRSSGSTARLPESVDHPLPPPTATDRTALVGQTPAPPPPRRGRGVEAR
                     MLASGGP"
BASE COUNT         2261 a         5558 c         5621 g         2224 t
ORIGIN      
        1 tccgggcgtc ccgctcgaga cgctccgcga ggcgctcggc cgatgcgcgc cgccgcgcgt
       61 cgcgccggaa ccgcgcccgg aggagtttcg cggccacgtc gtgcgttcgc cgcaccccga
      121 gagccctccg gagatcccgc gcggccgtct tgaggccggc ccccgagccc ttcgcgggga
      181 catcctgcgt gaggcgggcc agcgagacgg ttcgccggag ggcgacgcgg acggcgtgca
      241 gccgctcgcc ggtgggggcg ccggcgcctc ccgcgagaag cgtttcgagc gcggcgcggc
      301 gcttcgagag ggccgccgtc agggcgtgaa cgcggcggac ggtgccttcc acgtcatccc
      361 tcggccgcgg cgacgcggcc cgaaaagagc gcaagctggt ccgtgttcgc gtcgtactcg
      421 aagacctgga tcgcgcggcc ccagttcacc atcgtctcga agagctcgga agcgctgacg
      481 gtcgggaggc tcgccgcgag cgtgtcgcgc agggtttcgc ccggcaccgg ggtgtccgga
      541 tcgcgggcga gctcgcgcac gaggaggcag aaagtgccca gggacaggat ctgcggcctg
      601 aggagcggaa tcttcgcgtc gagcggcgcc gcgacgaccc tccttcccag atcggtgaga
      661 agaacgtcgt tcttcggggt gtcgacgagg tcgagcagct cggcggcctt cacgaccgtg
      721 agcgtccggc cgaagtcgta ttccgtgagc gcgtccacgg cgaagatgtt catcgttccg
      781 ccgttgtccg cgaggatctc gagaaggccg aggatctcgc ccatgcgggc aggcggcaac
      841 gggatgagcg gcgtctcgac ccggcggccc gggatccggg cggcggcctc ctcctcctcg
      901 ggaaggtgga cgcccgtcac gacgtcgtgg atgcgctcga ccatccggag gaactcgggg
      961 tgccggtact cgcgcgggtg cgggagcgtg ttgacgatct cgcggcgcac ggtgccggga
     1021 ttcgtgccga ggacgacgat gcgatccccg aggaacacgg cctcctcgat gagatgcgtg
     1081 atgagaagga ccgacgacag gcccgtgagg cgctccgtga aaagccggta gacctcggtg
     1141 cgcaggagct ccgccgtcag cacgtcgagc gccgagaacg gctcgtccat gcagaggagc
     1201 tcggggcggc ccacgagggc gcgcgcgatc ccgacgcgct gcttcatccc gcccgagagc
     1261 tccttcggaa acgcgcgcgc gtgcccggcg aggcccaccg ccgaaagcgc cgtcttcacg
     1321 cgctcgtccg cgtcccgggc gtccgtggcg cgtcccgcga cgccgacgcg tacgttctcc
     1381 tcgaccgtga gccatggaaa gagcgcgaag ttctggaaga ccacggcggc gcccgggtgg
     1441 atgccgacga gcggcttccc gtgctgctcg accgctccgg ccgacggcgg gatcaggccg
     1501 atgaggatgc gcaggagcgt cgacttcccg cagccggacg gcccgaggac ggcgacgacc
     1561 tcgttcggtc ggacctcgaa gtcgatggcc ttgaggatca cgcgcgcgcg tcccccctcg
     1621 tcctcgaaga tcttgtcgac gccccgggcc gaggcgatcg gagctgcggg ggcggggagc
     1681 gggttcgcgc tcatcggttg agagcgtact tcgtctcggc caggcgcgag aggcggcgcc
     1741 agaccgttcg gttgatgagg acgagcgcga aggacatcgt gagcactccg gccgcgagga
     1801 gcgggaagtt cgcctcccgg gtggccttcg tgatgaggga cccgaggccg aacgtctcga
     1861 gcgtccggcc gcccgagacc agcgtctccg cgacgatgga cgcgttccag gcgcctccgg
     1921 ccgcggtcac gaggcccgtc acgaggtacg ggaagacggc cggaaggaac agcgtcctcc
     1981 accgggcgcc tccggccacg ccgaagacgt cggccgcctc gacgaggtcc ggcggcaccg
     2041 cgctcgcgcc cgcgaggacg ttgaagagga cgtaccactg cgcgccgagg aacatgagaa
     2101 cggccgcgat cacggcgaac ggcacgtgga gcgcgagaag cgcgcccgtg acgatcggga
     2161 acagcatggg cgccgggaac gaggcgacga tctggacgat cggctggagg cgccgcgacc
     2221 aggccggcga acggccgacg aggatcccga ccgggagggt ccaggcgacg gcgagggcga
     2281 gcgcgccgag cgtcctcagg aacgtcgcgc cgagagcggc cacgaggagg agccagtcgt
     2341 gcggcgtcac ggcggagagg aggcgcacga gcttccacgc gcccaggagg gccgcggcgg
     2401 ccgcgccgac cccgaggacg aagaggagcg cggcgcgcag gcgcgccccc gcatcggcga
     2461 ttccggggac gctttccggc ggccgggcga gcggggcagc gggcgccttc gggcgccgcc
     2521 ggcgcctgag gcgcatgcgg cggaggacgg cggagcgccg gaggagatcg agcacccagg
     2581 agcgggcctc ggaagcgccc gacacctccg ccgcgcggaa gcgctgggac cacgcgacga
     2641 gagggcgcca gagcagctgg tccaccgcga cgatcatgac gaccatcgcg aggacggccc
     2701 aggccatcgc ggccttgtcg cccttctcga tcgcgacggc catgtaagag cccacaccgg
     2761 gcagccggaa gtcgcggtcg cccagcgtga acgcctcgtt caccgtgagg aagaaccatc
     2821 cgcccgccat cgacatcatc gagttccaca ccaggccgat gacggacgac gagacctcga
     2881 ccgtccggaa gcgcttccag aaggagaagc ggttcaggcg cgcgacctcg ttgagctcgt
     2941 tcgggatcga gcgcagcgac gcgatgtagg agaaggtcat gttccagacc tgccccgtga
     3001 agatcatgac gatgcacgcg agctcgaggc ccacgttcgt ccgcgggaag agggcgacca
     3061 tcccgaggac gagccccggc aggaagccga gaacggggat gccctgtccg atgtcgagca
     3121 gcgggatgag gactctctcg gcgcgtttcc cgtgggccgc gatggtcccg tagacgaggg
     3181 tgaagacgag ggagagcagg tacgccgcga agccgcgcga gagcgagagc agcgtgtacc
     3241 cgggcagggc cgacggcgag aggtcgatct cgaccgtcgg ccggatcggg gcctcccagc
     3301 gctgggcgag cgcgacgatg ccggcgacga gcgctccgac ggcaatgagg atcaggacgt
     3361 ccacggccgc gcccggggtt cgggcggcag gcttgcggac cctgaaggtc aggagcgcgg
     3421 tctccgcgtg agcgtcaggc tgcggcctcc ggtttcaccc agcgggtccg ccatcgggag
     3481 ctccggctcc gggaatccga tgtgccagcc ctgcgcccgg tccacgccga gctcgggcag
     3541 gatctccatg acgtcttcgg actcgacgcc ctcggcgacg gtcttgacgc cgaggccgcg
     3601 gcagatgccc gccattccgc ggacgaacac gcggttcgcc ttttcccgcg ggaggtcccg
     3661 aacgtagctg atgtcgagct tgagggagga gacgggcagc tcgcggagat agcggaacga
     3721 cgagaagccc atcccgaaat cgtccagcgc gaagtggaag ccctcggacg tgagcgcgtg
     3781 ggtgaagtcc cgcacgcggt cgaagtcgcg cagcgcggcc gtctccgtga tctcgaacgt
     3841 gacccgctcg ggccggatac ccttggcgcg cgcgaggcgc gagatgtccg cgacgagcgt
     3901 gtcgtcgtcg aagtcgaggc ccgagaggtt gatgccgatc tcgagctcgg ggctgatccg
     3961 cttgcgcgcg gtcgcgaggg cgtcgaaggc ggccgcgatc acggctcggt cgatcgccgt
     4021 gatgatcccg aaccgctcgg cggcgccgag gaactctccg ggcagggcga tccggccgtc
     4081 cggctcgcgc atcctgacga gcgtttcgac ggcgacgatg cggccggtgc cgacctcggc
     4141 gacgggctgg aagtagggga cgaaacggga ctcggcgagc gcggcgcgga tgcggttcga
     4201 ctgctcgcgc aggcggcccg tgcggtcgcg ctcgcggtcc gaggggtcgt ggagccgcac
     4261 gcgcgcgccg ccacggcgct tggcctggtg catagcgagg tcggccgcga ggaagaggtc
     4321 ggccgcgccc ccggcgtggt cggggaacac ggcgacgccc gcgctcgccg tgagcgacag
     4381 cgcgttgccc tcccagtcgg gcgtgagctt acggatgatg gagacgaccg cttcggcgcg
     4441 cacggtcgcc tccgacgcgc ccgttcccgg catgagcatt gcgacctcgt cgcctccgac
     4501 gcgcccgcac atctcgccgg gcagcagcga cggccggagc gtgttcgcca tcgcgcgcag
     4561 gtactcgtcc cccgcgagga gcccgtgggt gtcgttcagc gtcttgaagt tgtcgaggcc
     4621 caggatgatg agcgcgagcg ggcgggcgaa gaggtcggcg cgcgcgatct cgcgctcgag
     4681 ctcgaagtgg aactgctcgc ggttcgcgaa tcccgtgagg gcgtcggcgg gctccgcgac
     4741 ggtctcggcg gcatcgcccc gggtcccggc cgccgcgccc gtgacgtccc gcgccgacgc
     4801 gatgaagccg ccctcgcccg cctgcggggc gaagcacgtg gagatccagt acacgcgttc
     4861 gccgacgtcg atgccggccg ggaggcgcac ctcgaagttc ttcacgctgc ccgaggccgc
     4921 ggcctcgagg aacctcgtac ggccttccgg gtccctccag aacggacccg cggactcggg
     4981 cgggccgtcg acgctgccgg ggctcggcac gccctccgcc agggccacga cgttcgcgag
     5041 caggacctgc ccgtcgcggt cgagcaggac ggtcgcgtcg ggggagtcga cgaggagagt
     5101 cgccggccgt cggatcacgc cggaggcctt gcgcccttcg cgcaacttga tcggctgcgt
     5161 gtcggtctgc gacttcggtt tctgcaacgg ggtccttccc gagggctggc cttgactccc
     5221 gattatcgcc ccagagcctc cagggcgtca cccgtgaggc ggtacgttcg ccatccgtcc
     5281 atcgaaacgg cccccagagc cttgtagaag ccgatcgcgg gctcgttcca tgtcaggacg
     5341 ctccactcca ttcgcccgca tccccgctcg acggcgatcc gcgcgaggcg ctcgagaagg
     5401 gccttgccgg cgccgagccc ccggcgctcg ggtttcacgt agaggtcctc gaggtagagg
     5461 ccggggcgcg tgagccacgt cgagtagttg tggaagaaaa gcgcgaagcc gatgagctcg
     5521 cctccggtct cggcgacgat cgcctcggcg tagggcctgg gaccgaacag gtgcttctcg
     5581 agaagcgccg ggtccgcgtg gcagtcctcc ggctggcgct cgtagagggc gaggtcctgg
     5641 atgagtccga cgatcgcggg aacgtcctct cggagcgcct cgcgcaggat gaaagccggt
     5701 gcgttcatgc gatcccccgt gtgcattccg ctcatgttac gagggaggtg ctaaaaccgc
     5761 tgcgtggcgg cgagcggttc cttctacggg ctcctgacgc ggcgggtggg cgaggcccgc
     5821 cttgcggccg cgctcccgca cctgccgagg ggcggcggcg gcagagtcct ggacgtgggc
     5881 tgcggcctga cggatctcgc ggcgcggatc cccgactaca ccggcgcaga ccgcaatccc
     5941 gacgtgctcg ccgaacagag agggagattt ccgaagagag atttctatga atgggacatc
     6001 gttcggggcg cggctccggc ggcgctctcg gcgaaagcac cattcggggg aattcttctt
     6061 ctggccgtcc tcgaacacgt tcaggaaccc gcttcggtgc tcggacgtct cgcgccgctt
     6121 ctcgcgcccg gaggccggtt gatcgcgacg acgcctcatc cgagcggccg ctggccgctg
     6181 gaggcgggcg cggcgctcgg cctgctctcc tcccacgccc atgacgagca cgagacgctc
     6241 ctcggccgcg agtccctgga atcggcgggc cgcgcttcgg ggctgtctct cgttctctac
     6301 cggaggttcc tcctcggcat gaaccagctc gcggtctaca cacggtgacg gccggccccc
     6361 gcctcgtccg ccgcggcgcg gacgcgatcc atgtcgtgaa cgcccgcacg cacaacctca
     6421 gggacgtcac gtgcacgatc cccgtggggg cgctcaccgt cgtgacgggc ccttcgggtt
     6481 ccggcaagtc ttcgctcgcg ttcgacacgc tctacgccga gggccagcgt cgcttcgtcg
     6541 agtccatgtc cacgtacgcg cgccagtacc tcgagcggct cgaacgtccg gacgtggacg
     6601 agatcacgca cgtcctcccg gcgatcgcga tcgagcagag ggcgcccgcg cgctcggcgc
     6661 gctcgacgat cgggtcggcc accgagatcc acgacgtcct gcgcctcctc ttctcggccg
     6721 cgggcacgct gcgctgcccg gacgacggca gccccgtgcg ccgcgagacg gccgagagcg
     6781 tccgggcggc tcttctcgaa gagttcggcg agggcgcgcg cctcctcgtc gtcgcgcctc
     6841 gcagagtgac gtcgttcgcg gcggagagcg cggagtggag gaggcttggc ttctaccggt
     6901 acgtcggagc tgcgggcgac gttctggaaa tagaagagaa gatttcttcg agtgaaagag
     6961 agagggagag cagctcgccg ccccgcaggg ccgtagcgcg ttcgagcgcg gcgcgtcgtc
     7021 cctcttcacc cttcaaagag atcccctctt cctccctctt ccctctgctc atcggccgcc
     7081 acgtactgcg ggctgacgat ccggaaatcc tctcttcact ttcaacggcc ttcgacgccg
     7141 cagacggatc gcttctcgtc ttgaagtccg gagagggtct ttcctccgcc cgcacgttcc
     7201 gccgcggcct gtcgtgcaac gcgtgcggcc gggcgttcgc ggacccggtg cccgcgctct
     7261 tctcgttcaa ctcgccgcgc ggggcctgcg agacgtgcca ggggttcggg cggatcgtcg
     7321 gcatcgacgc ggcgcgcgtc attcccgacc cgcgcaagac gctgcgcgag cgtccgatcg
     7381 caccgttcaa ctcgccctcg tacgagagcg cgtacgacga cctcaaggcg gcgtcgcgcc
     7441 gcctcaagct gcggtgggac gttccctgga acgagctgac gcagcgggag agggacgccg
     7501 tctggacgcg ctccggagat tggtacggcg tcgagggcct cttcgagtac ctcgagaaga
     7561 agcgctacaa ggttcaggtt cgcgtcatgc tcgcgcgcta ccgcggctac tcgacgtgcc
     7621 ccgagtgccg cggcgcgcgg ctgaggcccg aggcgctcgc cgtgacggtc ggcggaaaga
     7681 cgatcgccga ggtctgcgac ctgacgctcg acggattcgt ggcgtttctc ggggcgctcc
     7741 cgctgcgcga agccgagcgc gagcgggcag cttccctcgt cgaggagctg gcgcggcgcg
     7801 cggggacgct cgtcgagatc ggcctcggct acctgacgct cgcgcgcacg atgcgcacgc
     7861 tgtcgggcgg tgaggctcag cgcgtccagc tcggctccgc gatcggcaac gcgctgaccg
     7921 gaacgctcta cgtcctcgac gagccgacgg tgggcctgca tccgcgggac acgcggcggc
     7981 tcctcgcggt cctcgagagg ctcgcggccg cgggcaacgc ggtcgtggcc gtcgagcacg
     8041 acacggacgt catccgtgcg gccgaccacg tcatcgacct cggcccgggg gccggcgcgc
     8101 tcggcgggcg gctcgtcttc gaaggaacgc cggaggagct gaccgaggcg gacaccgcga
     8161 cgggaaggtc gttgaagaag gagaaagaga tacttcttgc ttcggacgcg gcgggatacg
     8221 ggccgactgt ggcgacggac gccgtggctg cggagacggt gggagccttc ggcactttct
     8281 ttgaaggtgg agtgagtgca ggcgcgcccg agaggccgga gggccgcatt cgccccgccg
     8341 cccattcacc ttctaaggaa aaccatcgtg acgattcgat tcgcgtgcca ctcgaatcga
     8401 tacaggtcgt gggtgcgcga gcaaataatc tccagaacct ctcggtctcg tttcccttgc
     8461 ggcggctggt ggcggtgtcc ggggtgtcgg ggtcggggaa atcctccctc gtcgtcgacg
     8521 ttctcgccgc gggcgcgcgg cagcgcatgg gcaagggcct cctgccgggc atcgacgcgg
     8581 tgggggagca cgatggcatc ttcgggctcg agcacgtttc ggacgtcatc ctcgtcgacc
     8641 agtcgccgcc cgggcgctcc tcgcgctcga acccggcgac cgtgacgaag gcttgggacg
     8701 agatccggac gctcctcgcc cggacgcccg ccgcgaaggc gcgcggcctc gagaagggcg
     8761 ccttcagctt caacgcggcg ggcgggcggt gcgagaggtg cgagggcgcc ggcgtcgtga
     8821 ccgtggacat gcagttcctc gcggacgtga cgatcgtctg cgacgtctgc gacgggcgcc
     8881 gcttcaaacc ggaggtcctc gaggtgcgcc tgaggggccg gaacgtcgac gaactcctcg
     8941 cgacgaccgt cgacgaggcg cgcctcctct tcgcggacgc gcccgccatc gcgagccgcc
     9001 tccagccgct cgccgacgcg gggctcgggt acctcacgct cggccagtcg acggcgacgc
     9061 tctcgggcgg agaggcgcag cggctgaaga tcgcgacgtt cctccggggg gccgaggccc
     9121 cgaaggcgcc ggtcctgttc gttttcgacg agccgacgac cggcctggcg ccgtccgacg
     9181 tcgacgtcct cctcgccgtg ttccgccggc tcctcgcggc cggccattcg atcgtcgcgg
     9241 tcgagcacaa cacggccttt ctcgcgcgcg ccgaccacct gatcgagctg ggcccggacg
     9301 gcggcccgga cggcggccga gtcgtcttcg agggcgagcc ggcggcgctc gcggctcgcg
     9361 gcgggacgcc gaccgcccga gcgctgaaag gacgggtaca atttccgcca cggtgacgga
     9421 agagaagtgg agatacgttc tctcggccga aggccgcgag atcgagcttt cggacggaga
     9481 ggtgacgctg gggcgcagcc ggacgtccac cgtgcggctc gagcacgaat cggtttcgcg
     9541 ctcgcacgcg ctcctgacgt tcgaccgcgg cgaggcggtc gtgaaggatc tcgcgtcctc
     9601 gaacggcacg ttcgtgggcg gcaagcggat cgcgcgcgag acgcgcctcg tgaacggcga
     9661 ccgcctccag ctcggcgccg cggtgatcga agtccggctt ctcgcgccgg acgccgcgtc
     9721 cgagcgcacg gccctcatgg cgcccgacca gctgcccgac gcgcttcctc cgcgcccgcc
     9781 ctcgggcatc acgtacgagc cggtccccgg gctcacgaac ccgggactgg ggcccgcgac
     9841 gccgctcggg ccggacccga tctcggcatc ggaccttttc cgggccgtgg accgtccgcc
     9901 cgtggccgag ccttccctcg tcgtggacgt cttcccgccg gcggggggcg cgcccgacga
     9961 ggtctccacg atcgcgccgc cagcgatcgc gccgagcgcg gtcgcgccgc ccgcggttgc
    10021 gccgccgcct cgcgagctgg ccgactactc gattcgcgtc cacgaggccg agaaggcggc
    10081 tcgccttccg ctcggcgcca gagccgcgcc cttccgggat cgccgcgcgc cctcccggaa
    10141 cgagacggcg cggagcgtgg cgaacccgtt cgcccgcctt ctcgcggcgc tcgtcgacgc
    10201 cgtgattctc gccgcgatca acgtgctgct cttcgcgccg gtgttcctga tcgactactt
    10261 ccgctccgag ctccagacgc acgacgccgc acctgactgg gcgtttcgcg cgatcacggg
    10321 cctctcgatc ctcctcgcgc tcctcgcgaa cgtcctctac gtggtcggcg gctgggcgtg
    10381 gcgcggccgc acccccggca agtcccttct cggcctcgcc gtcgtcaagc gcggcgcgcc
    10441 ctcgggccag ggcatcggct ggccgtccgc gttcgttcgc gcgatcgtct gcctcctcgc
    10501 gggcctcccg ctggggctcg ggtgggcttg ggccttcttc gagaaggaga ggcgggcttt
    10561 ccaggatctc gcggccggaa cctgggtgat cagggtccgt tgagcgccgc gaggagcgcg
    10621 ggagacagga gcgcctcctc caccttcctc gggccgcctc gctcgcgcca ggcgccgagc
    10681 gagcggcgcg cccgggtgag gaggatcggg tcggactcgc cctccagaac gtagcgcgtc
    10741 ccgagcacga gacactgcag gaggtcggcc tcccgcgcgt caggccgcgc ggcccggagc
    10801 cccgcgagag ccgcctcggc gccctcgaag tcgcccgccg agaacaggac gaggactctt
    10861 tcgatcgtcg ccccaccggc gtccgcccgg gtcggacggg gcgtaggagc gagcagcggg
    10921 ggcgcgctcg tcggggccgg cgtgggaacg ggggggacgg gcgtgggcgt cgcggcggcc
    10981 tcgagagcgg ccgcggcgcg ccggtcgagc gtgtcgaccc gggcgaggag atctccgtat
    11041 tccgggaacc gccggatctc cccggcgtcg cgtgagcggg cgagatcctc gcgggcgccc
    11101 gcgtcgtccc cgatctccat ccttgccgct ccccgccagt agtacgggtc gtacaggtcg
    11161 acgtagtagc ggttggtccc gaagtgcacc ttgacgtctc gatggccggt gccgagcgcc
    11221 tcgtccattc gcttcaggac ctcgggccag tccttccgcg ccgcggccgc gacacccgcc
    11281 tggtaggctt tgaccgccgc gtcggggggt agaatcgact gcggcgcggc ggggaccgcc
    11341 agcgccagga aaagcccggc aagcgccggg atccggccgg aaacgcgtgg ggtggggacc
    11401 gcgacgggac actccctcgg aagcattcag aagggattct agagaacgat gggtttcccc
    11461 acccgattca cgtccggcct cgcgatcgcc atgctgctgg ccgccgtttt cggcgtccgg
    11521 caggccgggg cccagacgac gccgtacccg tgcccggccg ccgctcccgt cctcacaggc
    11581 cccgccctgg cgcaggcggg cgagacgtac gccgtctcgt ggacgcacgt cctcgggaac
    11641 ttcgtgtcct cgaacgccgc gtactcctac acgatcctcc gcgcagcgga caaggacttc
    11701 acgaaggtca ccgactcgat cacgacccag cgctccgcgc tgacgctgcc ggcgccgccg
    11761 gccggcgcgg cgactcttta tcaccaggtc gtcgtctcga cgccctgcac gacgctcggg
    11821 gcttcgttct cgaacgtcct cgccgtcccc gtcaagacga cgtgcgacgt cccgccctcg
    11881 gtcggcgccc tcacggcctc gccggagaac ccgcccgcgt tttcgacgtg ggtcgtctcg
    11941 tggagcacgc tcggcaccgg cccgggcccc ggcgtcggcg tggccaacct gaggttccgc
    12001 ctccgccgga cgagcgcact cgagcccgaa ggcagggagt gggtcgtgga cggcggctcg
    12061 gcttcctttg cggggccgcc cggcgaatat gtgttcgagg tccgcgccga ggccgcctgc
    12121 ggcagcgtgg gcccgtggtc gcccccgaag agggtcacgg tcggaacggt ggccaagccc
    12181 acgctcgtcc tcgtctccga gcccgagccg ctcgcgcgga tcgtgccggc cgcgggcgtc
    12241 aggccgggca cgtcgttcac ggtccgcaac ggaggaaccg agcccgtcac ggcgacgacg
    12301 aagtgcgccg acgcgggctt cgcgatcacc cctgcgacgt tcacgctccc ggcgggagcc
    12361 gcccaggaga tctcggtcac gtcgctgtac gtgaccgcgc ttgcccagcc cgtgaagacc
    12421 gcggtcacgg tttcctccgg cgcctcgacg ctcagcgtcc cgatcacgat cgtcttggcc
    12481 gccgcggccg cggccgctcc ggtccggtgg agtcagccga ccgcggacgt cgacgtgaac
    12541 ggcacggcgg tgacacggag cgtcgtgaac ggcggcgcga cgaccgcggc cttcgtcgcg
    12601 tcggtcaagg cgccgtggat cacggtccag tccgtcgacg ggcaggcgtg ggaccgcccg
    12661 ctcgcgccgt tcgagacacg ctccctgcgc ctcgtcttcg accgcgcgaa gcgccgcggc
    12721 gagaccggga ccgaggtggg cgcggtcgcg ctcacgacgg tcggcttcac cgcgcccgag
    12781 acgctcgtcg tgaccgacga cggtccgagg atcccgccgg ccgggacggg tgcgggcgcg
    12841 atcccggtcg cctccgcgaa gacgcgtgtc ctctacgcct cgttcccgaa cgcactcgac
    12901 gcccgcggcg tgggccgctt cgcggcggac ctctggatca cgaacacgga cgccgtgaat
    12961 ccgatcacgc tgtcgctcct cttcagcccg gtcggggcgc ccgccgacgg ctcggcactc
    13021 aagcgcttcg acgtccagct cgcggccggc gagacgcggc gctaccgcaa cgtcgtcgcg
    13081 acgctcatgg gctccgaggg cgcgtacacg gtcgaaatcc gctcctcggc gcccaccgtg
    13141 agcgccacgg ccctcgtcgc gaacacggcg ctcccggccg tcgcggccgc gcgccgggcg
    13201 cagaccggcc tcgcctccgg ctcgtacggg ttcgagatgc ggccaacctc gccgggcgag
    13261 ggggcgaagc agaacgaccc gatccaggtc acctccgggc tcgcgcacga cgcgaatcgc
    13321 cggtcgaatc tgctcctcct cgaaacatcg ggttacgaca cgacggttct cgtcgagctg
    13381 ttcaacaacc aggggaaacc ggtcacgaag aacggccagc cggtccagct ccagaagacc
    13441 gtgccggcga gcgggacgct ccagatcaac gacgccgacg agctcttcga cgcggcgccg
    13501 ctcgcgggca cgtacgcgta cgcgcggatc acgtggaaat cgagcgcgac ggacggaacg
    13561 ggcgcgcaga agggctccgt ggtcgggatg gcgacggtca tcgacaaccg cacgcaggac
    13621 tcgtcgctcc acgtgggcgt gtcgacgaac gcgctggatc ccgcctacgt gcccacgtcg
    13681 agcctcgaga cctcgcgcac cgcgctcgtc tctctgccgt tcggcggcgc tccggccccg
    13741 cttctttttc cgacggtcca ctcggccggc gccgcgctcg cgacgggggc gaagccgttc
    13801 tggcgcaccc gcgtgacgct cacgaacaca tcggaggcgc agcgatccgt cacgctcgag
    13861 tacctcgacg cggtcgaccc ctcggtcacg cggcagttcg gcgtgggcct ctcgccggga
    13921 ggcatcttct ctttcgagga cgtcctcgag gagaggttgg gcattctgcc gggacagaac
    13981 gcatacggag ccgtcaagat ctcggccgcg aagaacaacg acgaaacgtg ggccggcgtg
    14041 gacgtgcaga ccgagaccta caccgtagac ccggtgcagg gagcgggcga ctacaagacc
    14101 ggcatggaag gctacgcgta tttccacggc tactcgtcgt tccagtcgaa cctcgggacg
    14161 atgtcgttcg acggcgccga gaacagcacg ggttaccgga cgaacatcat cctgcacgag
    14221 gtcggaaacg cgtactgtga cgtcatcatc tcggcgtacc tgccggggtc gttcgtgccc
    14281 gtcgcgtcca agacccatcg cctcgccccc ttcggatact tctccgacga gctcttcacg
    14341 aacgtcctcg gcctgaacct cacggaggtc acggacgtgc gcgtcgtcgt gcggcagatc
    14401 gcgggggacg gcgtcttcct cgccttcgcc tcgaagatcg accgcgcgag cggcgatccc
    14461 gcgaacgtct tcctgcgccc cgccgccgcc ggcacgggcc gctgacgggt ccttggccgg
    14521 cgccgtcgaa cggatcgccc actaccggat cctgaagcgg atcggccgcg gcggcatggg
    14581 aatcgtgtac caggccgtcg acgagcgcga cggccgcctc gtcgccctga aggtcatccg
    14641 cgaggccgag gcccccggcg acgagtccgc ggtccaccag cacgaggcgc gcgtacggtt
    14701 cgaccgcgag gccgcgatcc tcaagagcct cctccacgtc aacgtcgtga ccttctacga
    14761 gatcggcgag gaggacggca cgccctggct cgcgatggag tacctcgacg gcgtgccgct
    14821 gacgtcctac gcgggcaagc cgtggacgga gacgctcccg ctcctcgtcc aggccgcgca
    14881 cgggatggag tacctcgcct cgcgcgggat cgtccaccgc gacctctctc ccgacaacat
    14941 cctcgtgatc gagcgcggcg gcgacctcat cgtcaagctc ctcgacttcg gaatcgcgaa
    15001 gctgttcgag cacgcgaccg gcctcgagtc gctcacggcg acgggtttct tcctcgggaa
    15061 ggtcgcctac ggctcgcccg agcagctcgg ctcgctcggc catggcgcga cgctcgactg
    15121 gcggagcgac gtctacagcc tcggcgtcat cttctaccag gtcctcgcgg gccagcgccc
    15181 gttcgacggc aaggcgccgg tcgagtacat cgcggctcat ctgaacgcga cggcgcctcc
    15241 cgtcgccgcg ccgcacgacg cgcccgccct tcccgtgccg ctcgtccggc tcatcgggca
    15301 gatgctggag aagcggcgcg aggaccggcc cggcggctgg cgcgagatcg tggaccggct
    15361 cgtcggagtt ctccgctcct ccgggagcac ggcgcggctc ccggagtccg tcgaccaccc
    15421 gctgccgccg ccgacggcca ccgaccgtac ggccctcgtg ggccagacgc cggcgccgcc
    15481 gccgccgcgc cggggacgcg gggtcgaggc gcgcatgctc gcgagcggcg ggccgtaggt
    15541 ctgctgccgg agaccctcga ccgccatctg gtggctgacg ccgaggccga ggaggaaccg
    15601 gccggggaac gcctcgctga tcgcccgcca tgccgacgcg gcggcgggcg cgccgtgctg
    15661 cgcc
//
LOCUS       DAOMFY010000064        15508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1175_length_15508_cov_8.303760, whole genome shotgun sequence.
ACCESSION   DAOMFY010000064 DAOMFY010000000
VERSION     DAOMFY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1175_length_15508_cov_8.303760"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="metG"
                     /locus_tag="PLB01_07340"
     CDS             <1..444
                     /gene="metG"
                     /locus_tag="PLB01_07340"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008858863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HQQ77153.1"
                     /translation="ASPAAVPAAGGSTGALTAPEAPGSSPSKNSPLPPSSSESKIPID
                     DFLKLDLRVGEVIAAEKVEKSKKLMKMTVRIGEDVRSIVGGIALAYTPEQLVGKKFIF
                     VANLAPAKLMGVESNGMILAATIPETGEPSLLMVDPAVPSGAKVK"
     gene            453..1232
                     /locus_tag="PLB01_07345"
     CDS             453..1232
                     /locus_tag="PLB01_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQQ77154.1"
                     /translation="MPSSEMPADPAARANLERILASPTYTRAQEDLGFLARRELRGVR
                     LMLELEKADFQLDQEGVRTTVVVFGGARLKEGDRWYAEAREFGRIVSTTCQANGMCEF
                     VVTTGGGPGAMEAANRGAWDVGAKTVGFNIQLPHEQYPNPYITPSLCFQFHYFGIRKM
                     HLLMRARALVAFPGGYGTLDELFETLTLVQTGKMKHLPIVLVGQDYWEKLINWKFLVE
                     EGGIDPEDLDLISFAEGGAEAWAAIVDFYSRHPALPGPLLR"
     gene            1232..2056
                     /locus_tag="PLB01_07350"
     CDS             1232..2056
                     /locus_tag="PLB01_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQQ77155.1"
                     /translation="MPFEYADSHGHLTMVFDGGEMKGAPRAPRPDEVKLLVTRAIDAG
                     VTRILVPSTDLEDQRRAVDTAERFDGVYAAVGFHPHEAKEFDEDRDLPLFEELCASKK
                     VVAVGEIGLDYHYDHSPRDVQKRVVAAQLRFARSRNLPVLLHNRLSEADLLPILEAEY
                     PGTPEAPRGVFHSFCADVATGERALALGYLVSFSGMLTFKTADNVREAAAALPLGSTL
                     LETDAPYLAPAPLRGKPNESSYLPRTAARLAEVKGVSVEEIARATTENFMRLFRVS"
     gene            2066..2440
                     /locus_tag="PLB01_07355"
     CDS             2066..2440
                     /locus_tag="PLB01_07355"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HQQ77156.1"
                     /translation="MARPPRLTRAEFEKLVEKAIAELPEEFRSRIENVVIAVEDEPTD
                     EDYELTDTPDDEDLFGIYRGAMRTQMTWDMLPDLPPQVVVFRGPILRNTDTSREAVRE
                     IKDTLIHELGHYFGLEDDEMPY"
     gene            complement(2441..2830)
                     /locus_tag="PLB01_07360"
     CDS             complement(2441..2830)
                     /locus_tag="PLB01_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQQ77157.1"
                     /translation="MILLDTHAWIWWAAETPRRLSARARRAIADSPSVGVSAISAWEV
                     AMLVAKGRLELDRDVLVWIRQALSLPRVTLVPLTPEIAVRSTRLGDGFPGDPADRILV
                     ATARELGAQLVTKDRALRRVEGVRIVW"
     gene            complement(2827..3054)
                     /locus_tag="PLB01_07365"
     CDS             complement(2827..3054)
                     /locus_tag="PLB01_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQQ77158.1"
                     /translation="MKAARTVPAGTFKAKCLALLDEVAETGRPIVVTKRGRPVARVTA
                     AVPESIRSLKGSVRSERDLVRPTGEVWRAAR"
     gene            complement(3088..3876)
                     /locus_tag="PLB01_07370"
     CDS             complement(3088..3876)
                     /locus_tag="PLB01_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77159.1"
                     /translation="MRTLGVLLLLAAASPAPAATEVVLRTAATHPMKYHLSLPEGWTA
                     GKTWPVAVVIPDASRDFEATLAAFVKARGSLPYILVAPHVVTSGGANYRLSDTYRYSD
                     ADWKRVASDGDWRFDDDGIAAVLADVRKEYGGEEKVFLTGWEAGGHTVWAYTFRHPDR
                     LRASAPVSPNWRGRWIGNADWSTAPGRARLPVKVLFCDRAKTEHPEGWDAWLGQTKEA
                     MDVAAAHGFRTVTLVNLPGRAHGPLAEDVIAFFEAVRSAAGRGP"
     gene            3944..4810
                     /locus_tag="PLB01_07375"
     CDS             3944..4810
                     /locus_tag="PLB01_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77160.1"
                     /translation="MKKSCALSLLVWLACTGVYGWLAWTKTREMVPAAVVGFLGGTFA
                     AMLVGSVAGLVTGGTDREALKRAVAGEPPKDGRLEAASGRVRPLGAPLEAPFTGRACV
                     AYEYDVKNPSAERSDFAGVALAPCAIDSPRGPVRILGWSMLDEFPKALQEEIDPARGL
                     AYLRSTTFEPLGLVNALSFFKNLVTDQDGSIRKDMRIADSEPDLEGKRIEEKVVPVGS
                     TVTVLGIWSEAERGIAPGSSAMLNRLYPVSPSSVLLDLRGSALKTFGIALVFFIALHA
                     ILVPMWFLAPAR"
     gene            complement(4832..5953)
                     /locus_tag="PLB01_07380"
     CDS             complement(4832..5953)
                     /locus_tag="PLB01_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77161.1"
                     /translation="MTRTRVLRLLAAALAAALGGCVSTEGTPGFAVSPKSWNLGVERR
                     GVDPAEAPNPMAVTPEMEAAALAIGGVGRDEDRLEHLRSALMDGGSWTFEYERNSTFS
                     AAEAFEKRRGNCVSFTNLFIAMGRSLGIRLHAALVSTRGTSERQGDLIVTYNHMIAVY
                     MEVNGRSAKVYDFYRMADQLTGRLTLLDDYMVAAIRASNDGIAHLGKGEGQQAVHDLE
                     IAVKLGPGLGSLYANLGLAKWRNGDVPGAFAALQKGLEIEPGSPPLLQNLAALYVEEG
                     RTAEARAALGALDLRRASPYALIVRGDLELRAGSPAGAIKNYREAASLDPKLADPWLA
                     IARAELARGRSDASRKAAKKALKRDPSSVEAQSLAGEAP"
     gene            6255..>15508
                     /locus_tag="PLB01_07385"
     CDS             6255..>15508
                     /locus_tag="PLB01_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ77162.1"
                     /translation="MSATGATPVAAQPGSPLGTAQLQVSGARLTLYADALTTDAEQTV
                     NVGEAARVRTCYGTGAACGTAAPGSVPGLKVVGDLSGPELPQAIPYETAPGGTFFLPG
                     FQREGDYLLSNVRLVETASGRVLANAEPSLATLHVRQILLASASVTRLTLADLQARGI
                     TITQQNFNAFKFAVGFVFSGTTVIIDLPVLYNGNGGAQPLAGPTVKLDGLPPAVASAV
                     RRWQPPNIIPFQLNATGPRVPKPGGADDEVTDPDYPLYGAIILPGSVSFLNQFFEAKL
                     IVANGAPNGSGATLQSVTGAMKLPSGEVLRLASTAPPVAVGQKLPVLNANGQAVMGPG
                     EQGTASWTVEGLLPGTHVLRMEIEADLARPGRPDLATGGVVQAAVEVVDARFQLAFNH
                     PDVVRTGEPYSLYVTVTNLSRAAQNLVTVDLATENISGAHKADAQESFAKTIATLAPG
                     ASETLEYRLVADVTGTCVATTFQATGGVSGVIRLRAGVGEAGIPLSPAALILPRFTNL
                     LPKSLVDADVRLLGLAYSLATAPAGATPAGLPHPLKADVERRAIDLGEAGQRLYLQEK
                     LLESLEALALDQLGNRHDLPEYDGLRRALDRGAQASTALGDLFRLEQQGRNLTAADFV
                     DHFAATASYGRPWMAAALVPNGAQPAPTLEVRQSSAAGTTYLASTSEESPRFRSLPYG
                     EIYAIHDVPGGAKMPFALVGRLDAAASYRVDLHAPAAVASGRLVLVVPSDDLSGFRKV
                     DFGFLTMGANEVWEVRAVKAAGDAALTFAIVYAGSGAPVPGAPAPSVTNLALPPFRVI
                     GAVQDPELDRYGLGISYLFNRPPDKASAETPSSYAVRSTFHGQDTASPAVTVDRVATK
                     AGAAAYWQPTSERVVNVRYQSPVSALNGIFESQPVIRHEHLLVTNAIRDVHGTPLDPL
                     VPAVVVDPNRTGGLVEGKVLRGDSTPAAGSRVQLLRWRKVVNPDAADAEFLFLDLVAE
                     VLAGADGAFYFDFVEEPPPPSRRIGHPPMSLVESVQSGFTLRAVVPAGADPVLQPEEK
                     EEVSTTIRLQNRLLHVNIALLGRGTVKGRLVHAEDGSPVTDGTVAAASTLFPEQRTVQ
                     PAADGSFTFGGLPVGPITLSGRDVAGNRVYQTIGIQRPGDVVNVTLQLGRTGPPKTGT
                     VSAKVMRLRSGSPRPPPIPSPGATVAVYSNGTFIASKFADNLGNATFTGVPAGKVTLQ
                     AADFSISRTPALTDLTLSADATVSATLTLAAAAPRSVVGRVLFHDGPTNTNVPVKGAV
                     AFIAGPGSFAYTDATGTYRIDGVPVQGVGEAAYTVTAFDNLRGLQGQTALPPVLDTGD
                     GTPVLAADILLKSMSGGIDGVVVDPLGRPYGGAAVDLGTDITTVARGDGRFSFDDIAV
                     GNWPVVAHVGDGLVAGKVGYFGQAMAAIVFGGHRPFVTIRMAGSGVVNVRTRTSASQG
                     VLSPITYRPTVYANGSIGALSGNPIQTTTDPDGRLALVLPVGGFSLTAVNPLNGNRSF
                     TRSIDYPGQVINLDIVFDSASTVTGRVVGVDGVTPVPNADVTFRAGGLLPQVQRTDGQ
                     GVFRYELVPRGAVSVTAAALVGSVDRVGRADGVITGPGQTLDLTVVMKAQGTVRGRVV
                     DVTGGVPAPLAFAQFYVQESGFPNRRLPAGTGFFTADAQGAYEVSHVFAGGVTVVARD
                     RNQVTRQGSARGEITTDFQVLTLPDVVISTAVGSLGVTVRDPDSGGPVADAQITLSNG
                     DVTVADVNGQASFDALPLGTYSAHAFHAPTGRAGLTSGLRLQNAGDRVDAIVVLDTRG
                     QVAGTLWDDAAKTAAVGGGTVQLTGRTNGRSWGTPISALATTSSDAATLGQFLFDGIP
                     PGAYALAAGVTTSPRRAAASLATTPTAPVVSVDLVLEPALDRFVRLFESLAAGVSEVN
                     PANGIYALTLSQSAGCPPGCVYSFTTGSTFTPYPGHLYRFPNVLTSQSLAVSAQESSG
                     KLRTGKISGTGAFAGSGTSSDPYRLVLRAKGRVVVTVRDAVNQPAQANVTLDSAGGHY
                     EAATDAAGVATFDAVLSGSVYAAARVVATGFGGTASGTLQFDDQTLNLGITLAPAVLA
                     HGVVYHPPAGDVWNGDVATLMPESGAIVQIRDSGGTLQIVTTGSDGAYRFAGLRTGAY
                     AISATDALVVSLANGGGSLSGPNGNDNALPPLVLDASRPAIVSIVPPPGSVGVSRNAP
                     VEITFSEPLLASVLPSGGPTSPYFTLRAASGLSPAGAWTASTNPAGQQVVRFTPAGLY
                     DNTTVYSLTIAGGASGVRDRAGRPITDSGDVGSNFTTSDTVGPLVVGTVPTLDRPVDP
                     VAPIRFDFSEVVTATPQELDGSGPTPAAQLFWQQNGNPAWQPVPVTMFLSRGGYSLVV
                     QPPTGVTYQNDSFKRRVHLSRLKDASGNAMADYDRDFRVYDLNPPHVDVAFPAGAPTG
                     QLVGGSAYTLAPVLSSLDDLPQGDVDRVEYFLASSGDPSAPASTPAFTARTSPFGWTF
                     VAAYVGNGVNPRPFPVWVKATDTSTNASNVVKLAMAVLPNAPPVITSIAPAATAPVAG
                     TFYAGSALAATVAGVADPDGASVTLTAELRKDNVGSPNDPADLVASLPSQALAKPAAG
                     WAALPPPVFASAIPITTAEGTSLFFRVKATDSLGASVTAESLRFAVAHDPNAPSVDSF
                     VARLSGSASPSSQFFIGQKFVLEFRARDSETALRTTSIALSGVFPSPLLAALVGGTTN
                     LYRTVELTVPATVPPGGLAIAATASATDWGGNTGTSPLAFNVSPTPDPYAPAATWLTP
                     WESGAWPSGYTSTVSAQGAAMLLRVKVSDLDRVSNVDVPGTIASVQFKGPADAAGTLA
                     TAFVDGVLVAGTGGPGTGIYEALWRVPNGVASGTQLPFQVRVVDAGANATVADVRLRA
                     VPPRKVYEAAQVAALPADTMLGAGGDAAGPVFLLDGTTLSLYPQTAPAVRALASMFVY
                     AGGVAAGATFTPTASVLTAPEVTSYASSVLYNPLELTVTDTFGLGHGARVDVSAKGLL
                     GSTPTQAMVLPGQTGSQQNAGGSHGGSGGPGSPLGWTRTDLYASGSVFDSVKDPALPG
                     GGAGYATGPFGATAAGGTGGGVVRL"
BASE COUNT         2135 a         5658 c         5436 g         2279 t
ORIGIN      
        1 gcgagccccg cggcggtgcc ggcggccggc ggcagcacgg gcgcgctgac agcgcccgag
       61 gccccgggtt cttcaccttc taagaactct cctcttcctc cctcctcgtc cgaaagcaaa
      121 atccccatcg acgacttcct gaagctcgat ctccgcgtcg gcgaggtgat cgccgccgaa
      181 aaggtcgaga agtcgaagaa gctgatgaag atgaccgtca ggatcggcga ggacgtgcgc
      241 tcgatcgtcg gcgggatcgc gctcgcctac acgcccgagc agctcgtcgg gaagaagttc
      301 atcttcgtcg cgaacctcgc gcccgcgaag ctcatgggtg tcgagtcgaa cgggatgatc
      361 ctcgcggcca cgatccccga gacgggagag ccgtcgctcc tcatggtcga cccggcggtc
      421 ccgtcgggcg cgaaggtcaa gtaatgaccc gcgtgccttc gtccgagatg ccggccgacc
      481 cggccgcgag ggcgaatctc gagaggatcc tcgcctcgcc gacgtacacg cgcgcgcagg
      541 aagacctcgg gttcctcgct cgccgcgagc tgcgcggcgt gcgcctcatg ctcgagctcg
      601 agaaggccga cttccagctg gaccaggagg gcgtccggac gacggtcgtc gtcttcggcg
      661 gcgcccgcct gaaggaaggc gaccgctggt acgccgaggc gcgggagttc gggcgcatcg
      721 tctcgacgac ctgccaggcg aacggcatgt gcgagttcgt cgtcaccacg ggcgggggcc
      781 cgggcgcgat ggaggccgcg aaccgcggcg cgtgggacgt cggggccaag accgtcggct
      841 tcaacatcca gctcccgcac gagcagtacc cgaatccgta catcacgccg tcgctctgct
      901 tccagttcca ctacttcggc atccgcaaga tgcacctcct catgcgggcc cgggcgctcg
      961 tcgccttccc gggcggatac gggacgctgg acgagctctt cgagacgctg acgctcgtcc
     1021 agaccgggaa gatgaagcac ctcccgatcg ttctcgtcgg ccaggactac tgggagaagc
     1081 tcatcaactg gaagttcctc gtcgaggagg gcggaatcga tcccgaggac ctcgacctca
     1141 tctccttcgc cgaaggcggc gccgaggcct gggccgcgat cgtcgacttc tactcccggc
     1201 atcccgccct ccccggtccg ctcttgcgct gatgcctttc gagtacgcgg actcgcacgg
     1261 ccatctcacg atggtcttcg acggcggcga gatgaagggc gcgccgcgcg ccccgcgccc
     1321 cgacgaggtg aagctcctcg tgacgcgcgc aatcgacgcg ggcgtcacgc gcatcctcgt
     1381 tccctcgacg gacctcgagg accagcggcg cgccgtggac accgccgagc gtttcgacgg
     1441 ggtgtacgcg gcggtgggct tccacccgca cgaggcgaag gagttcgacg aggaccggga
     1501 cctcccgctc ttcgaagagc tctgcgcgtc gaagaaggtc gtcgccgtgg gggagatcgg
     1561 cctcgactac cactacgacc actcgccgcg cgacgtccag aagcgcgtcg tcgccgcgca
     1621 gctgcgcttc gcgaggagcc ggaacctccc ggtcctcctc cacaaccggc tgagcgaggc
     1681 ggacctcctg ccgatcctcg aggccgagta ccccggcacg ccggaggcgc cgcgcggcgt
     1741 cttccactcc ttctgcgcgg acgtcgcgac gggggagcgg gcgctcgcgc tgggctacct
     1801 cgtgagcttc tcggggatgc tcacgttcaa gaccgccgac aacgtgcgag aggcggcggc
     1861 cgcgctccct ctcggctcga cgctgctcga gacggacgcc ccgtacctcg cgccggcgcc
     1921 gctgcgcggg aagccgaacg agtcgagcta tctcccgcgc acggccgcgc gcctcgccga
     1981 ggtcaagggc gtctccgtcg aggagatcgc gcgcgcgacg acggagaact tcatgcgtct
     2041 cttccgcgtg tcctgaggag gtcccgtggc ccgcccgccc cgcctgacga gagccgagtt
     2101 cgagaagctc gtcgagaagg cgatcgccga gctgcccgag gagttccgct cgcggatcga
     2161 gaacgtcgtg atcgcggtcg aggacgagcc gacggacgag gactacgagc tcacggacac
     2221 gcccgacgac gaggatctct tcggcatcta ccgcggggcg atgcgcaccc agatgacgtg
     2281 ggacatgctt ccggatcttc cgccgcaggt cgtcgtcttc cgcgggccca tcctccgcaa
     2341 caccgacacg agccgcgagg ccgtgcgcga gatcaaggac acgctcatcc acgagctcgg
     2401 ccactacttc gggctcgagg acgacgagat gccgtactga tcaccagacg atccggacgc
     2461 cctccacgcg cctgagggcc cggtctttgg tgacgagttg tgcgccgagc tcccgggccg
     2521 tcgcgacgag gatccggtcg gcggggtcgc cggggaatcc gtccccgaga cgcgtcgagc
     2581 ggacggcgat ctccggcgta agcggcacga gcgtcacgcg gggcagcgaa agcgcctggc
     2641 ggatccagac gaggacgtcg cgatccagct cgagccggcc tttcgcgacg agcatggcga
     2701 cctcccatgc ggagatcgcg ctcacgccga ccgaggggga gtccgcgatc gcgcggcggg
     2761 cccgcgcgga gagacgccgg ggcgtctcgg cggcccacca gatccaggcg tgggtgtcga
     2821 ggaggatcac cgggcggcgc gccagacctc gcccgtcgga cgcacgaggt cgcgctcgct
     2881 ccggacgctg cccttgaggc tgcggatcga ctcgggtacg gcggcggtca cgcgggccac
     2941 cggccggccg cgcttcgtca cgacgatcgg acggccggtc tcggcgacct cgtcgaggag
     3001 ggcgaggcac ttcgccttga acgtgccggc cgggacggtg cgcgcagcct tcatggtcac
     3061 gagaatatgg tcacatcggg ggggccgtca aggcccgcgc ccggcggcgg agcgcacggc
     3121 ctcgaagaac gcgatcacgt cctccgcgag aggcccgtgc gcgcggccgg gcaggttcac
     3181 gagcgtgacg gtcctgaatc cgtgggcggc ggcgacgtcc atcgcctcct tcgtctggcc
     3241 gagccacgca tcccatccct ccgggtgctc cgtcttcgcg cggtcgcaga agaggacctt
     3301 cacgggcagc cgtgcgcgcc cgggggcggt cgaccagtcc gcgtttccga tccaccttcc
     3361 tcgccagttc ggcgagacgg gagcggaggc gcgcagccgg tccgggtgtc ggaacgtgta
     3421 cgcccacacc gtgtgcccgc cggcctccca gcctgtcagg aacaccttct cctcgccgcc
     3481 gtattccttc cgcacgtcgg cgaggaccgc cgcgatgccg tcgtcgtcga agcgccagtc
     3541 gccgtcgctc gcgaccctct tccagtcggc atccgagtac cggtacgtgt ccgagagccg
     3601 gtagttcgcg ccgccgctcg tgacgacgtg cggtgcgacg aggatgtacg gcagcgagcc
     3661 gcgcgccttc acgaacgcgg ccagggtcgc ctcgaagtcg cgcgaggcgt ccggaatcac
     3721 gacggcgacc ggccacgtct tcccggccgt ccagccctcg ggcagtgaga ggtggtactt
     3781 catcggatgg gtggccgccg tccggaggac gacctccgtc gcggccggcg cgggggaggc
     3841 ggcggcgaga aggagaagaa caccgagcgt tcgcacgcgc gttctacgcg tttcccgacc
     3901 cgcgggtttc gggcgtgcac cgtgcatggg agaattgttc tcaatgaaga agagttgcgc
     3961 gctctccctc ctcgtctggc tggcgtgcac cggcgtctac ggatggctcg cgtggacgaa
     4021 gacgcgtgag atggtcccgg cggccgtggt cgggttcctc ggcgggacgt tcgccgcgat
     4081 gctcgtcggc tcggtcgcgg gcctcgtcac gggcgggacg gatcgcgagg ccctgaagcg
     4141 ggccgtcgcg ggcgagccgc cgaaggacgg ccgcctcgag gccgcgagcg ggcgcgtccg
     4201 gcccctcggc gccccgctgg aggccccgtt cacgggccgc gcgtgcgtgg cgtacgaata
     4261 cgacgtcaag aacccgtcag ccgagcggag tgacttcgcg ggcgtcgccc tcgcaccgtg
     4321 cgcgatcgac tcgccgcggg gcccggtgcg gatcctcggc tggtcgatgc tcgacgagtt
     4381 cccgaaagcg cttcaggaag agatcgaccc ggcgcgcggc ctcgcgtatc tccgcagcac
     4441 gacgttcgag ccgctcggcc tcgtgaacgc gctctcgttc ttcaagaacc tcgtcacgga
     4501 ccaggacggc tcgatccgca aagacatgcg catcgccgac agcgagccgg atctcgaggg
     4561 caagcgcatc gaggagaagg tggtcccggt gggctccacc gtcaccgtcc tcgggatctg
     4621 gtcggaagcc gagcgcggca tcgctcccgg gtcgtccgcg atgctgaacc ggctctatcc
     4681 ggtcagcccg tcgtccgtcc tcctggacct ccgcggcagc gcgctgaaga cgttcgggat
     4741 cgcgctcgtc ttcttcatcg ctctccacgc catcctcgtg ccgatgtggt ttctcgcgcc
     4801 cgcacgctga agcggcccgc gaacctcgtc ctcagggcgc ctctcccgcc aggctttgag
     4861 cctcgacgct cgacggatcg cgcttgagag ccttcttcgc cgccttgcgc gacgcgtcgg
     4921 accgcccgcg cgcgagctcg gcgcgcgcga tcgcgagcca cgggtcggcg agcttggggt
     4981 cgagcgacgc ggcttcgcgg tagttcttga tcgcgccggc cgggctgccg gcgcgcagct
     5041 ccaggtcgcc gcgcacgatg agcgcgtacg gcgaggcccg gcgcaggtcg agcgcgccga
     5101 gcgccgcccg cgcctcggcg gttctgccct cctcgacgta gagggccgcg aggttctgga
     5161 ggagcggagg cgagccgggc tcgatctcga gccccttctg gagcgcggcg aaggccccgg
     5221 ggacgtcgcc gttcctccac ttcgcgaggc cgaggttcgc gtagagggac ccgagccccg
     5281 gcccgagctt cacggcgatc tcgaggtcgt gcacggcttg ctggccctct cccttcccga
     5341 ggtgcgcgat gccgtcgttg ctcgcgcgga tggcggcgac catgtagtcg tcgagaaggg
     5401 tgaggcgccc cgtgagctgg tcggccatcc ggtagaagtc gtagaccttc gcgctgcggc
     5461 cgttgacttc catgtagacg gcgatcatgt ggttgtacgt gacgatgagg tcgccctggc
     5521 gctcggacgt cccccgcgtc gagacgagag ccgcgtgcag gcggatcccg agcgagcgcc
     5581 ccatcgcgat gaagaggttc gtgaaggaga cgcagttccc gcgccgtttc tcgaaggctt
     5641 cggccgccga gaaggtggag ttccgctcgt actcgaacgt ccacgagcct ccgtccatga
     5701 gcgcggaacg caggtgctcg agccggtcct cgtcccgccc gaccccgccg atggccagcg
     5761 cggcggcctc catttcgggc gtcacggcca tcgggttcgg cgcctcggcg gggtccacgc
     5821 cgcggcgctc gacgcccagg ttccagctct tcgggctcac ggcgaagccc ggcgtcccct
     5881 cggtcgagac gcagccgccg agggccgcgg cgagcgcggc ggcgaggagg cggaggacgc
     5941 gggtgcgggt cattccctca atttacgccc cgcggggttg cggggttgcg ccctccgggc
     6001 ccgccggccc cgggccggct ggcctccctc ctcgtgccgt gatatgaagc acggccagta
     6061 gtttaggttc cggtctcttt ttcctcaggc cgagtcggaa agtccctcgg gggcggtcga
     6121 tggcgtttcg ccggaggcgc ggaggtctct cgggccagcc cgatcttgcg ggcccggaga
     6181 ggtctcgcct gcactccggc cgcgcaggtc acgacgcgct gggcattgtc cggacgatcg
     6241 ccgcttcgct cctcgtgtct gctacggggg caacgccagt tgccgcgcag cccggcagcc
     6301 cgctcggaac cgctcagctt caggtctccg gtgcccggct cacgctctac gccgacgcgc
     6361 tgacgaccga cgctgagcag acggtcaacg tcggcgaggc cgcccgggtc cgcacgtgct
     6421 acgggacggg tgcggcgtgc ggcacggcgg cgcccggctc cgtgcccggc ctgaaggtcg
     6481 tcggcgacct ctcgggaccg gagctgccgc aggcgatccc gtacgaaacg gcgcccggcg
     6541 ggacgttctt cctcccgggc ttccagcgcg agggcgacta tctcctctcg aacgtccgcc
     6601 tcgtcgagac ggcgtcgggg cgcgtcctcg cgaacgccga gccgtcgctc gcgacgctgc
     6661 acgtgcggca gatcctcctc gcgtccgcca gcgtcacgcg cctcacgctc gccgacctcc
     6721 aggcgcgcgg aatcacgatc acgcagcaga acttcaacgc gttcaagttc gccgtcgggt
     6781 tcgtgttctc gggaacgacc gtcatcatcg accttccggt cctctacaac ggcaacggcg
     6841 gggcgcagcc gctggccggt cccacggtga agctcgacgg actgccgccg gccgtcgcga
     6901 gcgccgtcag gcgatggcag ccgccgaaca tcatcccgtt ccagctcaac gcgaccggtc
     6961 cccgcgtccc gaagcccggc ggcgcggacg atgaggtgac cgatcccgac tatccgctct
     7021 acggggcgat catcctgccc ggcagcgtct cgttcctgaa ccagttcttc gaggcgaagc
     7081 tgatcgtcgc gaacggcgca ccgaacggct cgggcgcgac gcttcagagc gtgacgggcg
     7141 ccatgaagct gccgtccggc gaggtcctgc gcctcgcgtc cacggcgccg ccggtggccg
     7201 tcgggcagaa gctcccggtc ctgaacgcga acggacaggc ggtgatggga cccggcgagc
     7261 agggcacggc gagctggacg gtcgagggcc tcctgccggg cacgcacgtc ctgcgcatgg
     7321 agatcgaggc ggacctcgcg cggccgggcc ggccggacct cgcgacgggc ggtgtcgtgc
     7381 aggccgccgt cgaggtcgtg gacgcgcgct tccagctcgc gttcaaccat ccggacgtcg
     7441 tgcgaacggg cgagccctac tcgctctacg tcacggtgac gaacctctcg cgcgcggcgc
     7501 agaacctcgt cacggtggac ctcgcgacgg agaacatctc gggcgcgcac aaggccgacg
     7561 cgcaggagtc gttcgcgaag acgatcgcga cgctcgcgcc gggcgcgagc gagacgctgg
     7621 aataccggct ggtcgcggac gtgacgggga cgtgcgtcgc gacgacgttc caggcgaccg
     7681 gcggcgtctc cggagtcatc cgcctgcgcg ccggggtggg tgaggcgggg atcccgctct
     7741 cgccggccgc gctcatcctg ccgcgcttca cgaacctcct gccgaagagc ctcgtggacg
     7801 ccgacgtgcg cctcctcggc ctcgcctaca gcctcgcgac cgctcccgcg ggcgcgacgc
     7861 cggccggcct cccgcatccg ctcaaggccg acgtcgaacg ccgcgcgatc gacctcggcg
     7921 aggccggcca gcgcctctac ctgcaggaga agcttctcga atcgctcgag gcgctcgcgc
     7981 tcgaccagct cgggaaccgc cacgaccttc cggagtacga cggcctgcgc cgcgcgctcg
     8041 accgcggagc gcaggcctcg accgcgctcg gcgacctctt ccggctcgag cagcagggcc
     8101 ggaacctcac ggcggccgac ttcgtcgacc acttcgcggc gacggcgagc tacggccgcc
     8161 cgtggatggc ggcggcgctc gtgccgaacg gcgcgcagcc cgcgccgacg ctcgaggtga
     8221 ggcagtcctc ggcggccggc acgacgtacc tcgcgtccac gagcgaggag tcgccgcgct
     8281 tccggagcct tccctacggc gagatctacg cgatccacga cgttccgggc ggcgcgaaga
     8341 tgccgttcgc cctcgtgggc aggctcgacg ccgcggcctc gtatcgggtc gacctccatg
     8401 cgcccgcggc ggtcgcttcg ggccgcctcg tcctcgtcgt gccgtccgac gacctatcgg
     8461 gcttccgcaa ggtggacttc gggttcctga cgatgggcgc gaacgaggtc tgggaagtgc
     8521 gggcggtcaa ggcggcgggc gacgcggcgc tgactttcgc gatcgtctac gcgggctcgg
     8581 gagcgcctgt ccccggcgcg ccggcgcctt ccgtgacgaa tctcgcgctc ccgccgttcc
     8641 gcgtgatcgg cgccgttcag gatcccgagc tcgatcgcta cggcctgggg atctcgtacc
     8701 tcttcaaccg cccgcccgac aaggcgtccg ccgagacgcc ctcgagctac gccgtccgct
     8761 ccacgttcca cgggcaggac acggcgtcgc ccgccgtgac ggtcgaccgc gtggcgacga
     8821 aggccggcgc ggcggcgtac tggcagccca ccagcgagcg cgtcgtgaac gtgcggtacc
     8881 agagcccggt cagcgcactc aacgggattt tcgaaagcca gcccgtgatc cggcacgagc
     8941 acctcctcgt cacgaacgcg atccgcgacg tgcacggaac gccgctcgac ccgctggtgc
     9001 cggcggtcgt cgtcgacccg aatcgcaccg gcggtctcgt cgagggaaag gtcctgcggg
     9061 gggactcgac gcccgccgcc gggtcgcgcg tccagctcct ccggtggcgg aaggtcgtga
     9121 accccgacgc ggccgacgcg gagttcctgt tcctggacct cgtggccgag gtgctggcgg
     9181 gcgccgacgg cgcgttctac ttcgatttcg tcgaggagcc gccgccgccc tcgcggcgga
     9241 tcggccatcc gccgatgtcc ctcgtcgaaa gcgtgcagtc cggcttcacg ctccgcgcgg
     9301 tcgtcccggc gggggccgat cccgtcctgc agccggagga gaaggaagag gtctcgacca
     9361 cgatccgcct ccagaaccgc ctcctgcacg tgaacatcgc gctcctcgga cgcggcacgg
     9421 tcaaggggcg gctcgtccat gcggaagacg gctcgcccgt gacggacgga accgtcgcgg
     9481 ccgcgagcac gctcttcccc gagcagcgca cggttcagcc cgcggcggac ggctccttca
     9541 cgttcggcgg gctgccggtc ggcccgatca cgctctcggg ccgcgacgtc gcgggcaacc
     9601 gcgtctacca gacgatcggc atccagagac ccggcgacgt cgtgaacgtg acgctccagc
     9661 tcgggcggac gggacctccc aagaccggca cggtgtccgc caaggtgatg cgcctccgga
     9721 gcggcagccc ccggccgccg ccgattccgt cgccgggcgc gacagtcgcc gtctactcga
     9781 acggcacctt catcgcgtcg aagttcgccg acaacctcgg caacgcgacg ttcacgggcg
     9841 tgccggccgg aaaggtcacg ctgcaggcgg cggacttcag catttcgagg acgccggccc
     9901 tcacggacct cacgctgtcg gccgacgcga ccgtttcggc cacgctcaca ctcgccgcgg
     9961 cggcgccgcg cagcgtcgtc ggccgcgtgc tcttccacga cggtccgacg aacacgaacg
    10021 ttcccgtcaa gggcgcggtc gcgttcatcg ccggccccgg cagtttcgcg tacacggacg
    10081 cgacgggaac gtaccgcatc gacggcgtgc cggtgcaggg cgtcggcgag gccgcgtaca
    10141 cggtgaccgc gttcgacaac ctgcgaggcc tgcaggggca gacggcattg ccgccggtgc
    10201 tcgacacggg ggacggaacg ccggtgctcg cggccgacat ccttctgaag agcatgtccg
    10261 gcggaatcga cggcgtcgtc gtcgatccgc tcggccggcc gtacggcggc gccgcggtcg
    10321 acctcgggac ggacatcacg acggtcgccc gcggcgacgg ccgcttctca ttcgacgaca
    10381 tcgcggtcgg caactggccc gtggtcgcgc acgtcggcga cggactcgtc gcgggcaaag
    10441 tgggctactt cgggcaggct atggcggcga tcgtcttcgg cggccacagg cccttcgtca
    10501 cgatccgcat ggcgggctcg ggcgtcgtga acgtccggac gcgcacgtcg gcgtcgcagg
    10561 gcgttctgtc gccgatcacg taccggccga ccgtctatgc gaacgggtcg atcggggcgc
    10621 tctccggcaa cccgatccag acgacgacgg atccggacgg ccgcctcgcg ctcgtcctcc
    10681 ccgtcggcgg cttctcgctg acggccgtca acccgctgaa cggcaacagg tccttcaccc
    10741 gctcgatcga ctatcccggg caggtgatca acctcgacat cgtcttcgac tcggcatcga
    10801 cggtgacggg gcgcgtcgtc ggcgtggacg gcgtcacgcc cgtgccgaac gcggacgtga
    10861 cgttcagggc gggcggcctc cttccgcagg tccagcggac ggacggccag ggcgtcttcc
    10921 gctacgagct cgtgccgcgc ggcgcggtct ccgtcacggc ggcggcgctc gtcgggagcg
    10981 tggaccgagt gggccgggcg gacggcgtca tcaccgggcc ggggcagacg ctcgacctca
    11041 cggtcgtcat gaaggcccag ggcaccgtgc gcgggcgcgt cgtcgacgtc acgggcggcg
    11101 ttccggcgcc gctcgcgttc gcgcagttct acgttcagga gtcgggcttc ccgaaccgcc
    11161 ggctgcccgc gggcacgggc ttcttcaccg cggatgcgca gggagcgtac gaggtctcgc
    11221 acgtcttcgc gggaggagtg acggtggttg cgcgtgaccg caaccaggtg acgcggcagg
    11281 ggagcgcgcg cggcgagatc acgaccgatt tccaggtgct caccttgccc gacgtcgtga
    11341 tctcgacggc cgtcgggtcg ctcggcgtga ccgtgcgcga tcccgattcc ggcggcccgg
    11401 tcgccgacgc tcagatcacc ctctcgaacg gagacgtgac ggtcgccgac gtcaacggcc
    11461 aggcctcgtt cgacgcgctg ccgctcggca cgtactcggc ccacgcgttc cacgccccga
    11521 cggggcgtgc gggcctcacg agcgggctgc gcctccagaa cgcgggcgac cgcgtggacg
    11581 cgatcgtcgt cctcgacacg cgcgggcagg tcgcgggcac gctctgggac gacgccgcga
    11641 agacggcggc ggtcggaggc ggaacggtcc agctcacggg ccgcacgaac ggccgctcct
    11701 gggggacgcc gatctccgcg ctcgcgacga cgtcctccga cgccgcgacg ctcggtcagt
    11761 ttctcttcga cggcattccg ccgggtgcct acgcgcttgc cgcgggcgtc acgacgtctc
    11821 cccgccgcgc tgcggcctcg ctcgcgacga cgccgaccgc cccggtcgtc tcggtggacc
    11881 tcgtactcga gcccgccctc gaccggttcg tccggctctt cgagagcctt gcggccggag
    11941 tctccgaggt gaaccccgcg aacgggatct acgctctcac gctctcgcag tccgcgggct
    12001 gtccgccggg ctgcgtttac tccttcacga caggatccac tttcacgccg tatcccggac
    12061 acctctatcg attcccgaac gtcctgacct cgcagagcct cgcggtttcg gcgcaggagt
    12121 cgagcggcaa gctgaggacc gggaagatct cgggcacggg cgcgttcgcg ggcagcggca
    12181 cgtcgtcgga cccgtatcgg ctcgtcctcc gcgcgaaggg ccgcgtcgtc gtcaccgtac
    12241 gcgacgcggt gaaccagccc gcgcaggcga atgtcacgct cgactcggcc ggaggccact
    12301 acgaggccgc gacggacgcg gcgggcgtcg ccacgttcga cgcggtcctc tcgggcagtg
    12361 tctacgcggc cgcgcgcgtc gtggccacgg gcttcggcgg caccgcgtcc gggacgctgc
    12421 agttcgacga ccagacgctg aacctcggga tcacgctcgc gcctgccgtc cttgcccacg
    12481 gcgtcgtgta tcacccgccg gcgggcgacg tctggaacgg cgacgtcgcg acgctgatgc
    12541 ccgagagcgg cgcgatcgtc cagatccgcg actcgggagg cacgctccag atcgtgacga
    12601 cggggtccga cggcgcctac cgcttcgcgg gcctgcggac gggcgcgtat gccatcagcg
    12661 cgaccgacgc gctcgtggtt tcgctcgcga acggcggcgg gtcgctctcc ggcccgaacg
    12721 ggaacgacaa cgcgctgccg ccgctcgtcc tcgacgcgtc gcggccggcg atcgtttcga
    12781 tcgtcccgcc tccggggagc gtcggcgtct cccgcaacgc cccggtcgag atcacgttct
    12841 ccgagccgct cctcgcctcg gtgctgccgt cgggcgggcc gacatcgccg tacttcacgc
    12901 tgcgcgccgc gagcgggctc tcgcccgcgg gggcgtggac ggcctcgacg aatcccgcgg
    12961 gccagcaggt cgtccgcttc acgccggccg gcctgtacga caacaccacg gtctacagtc
    13021 tgaccatcgc cggcggtgcc tccggcgtgc gcgaccgcgc gggccgtccg atcaccgatt
    13081 cgggcgacgt ggggtccaac ttcacgacgt ccgacaccgt cgggccgctc gtcgtgggaa
    13141 ccgttcccac gctcgaccgc ccggtcgatc ccgtcgctcc gatccgcttc gacttcagcg
    13201 aggtcgtgac cgcaacgccg caggaactcg acggctcggg gccgacgccg gccgcgcagc
    13261 tcttctggca gcagaacggg aaccccgcct ggcagccggt gccggtcacg atgttcctgt
    13321 cgcgcggcgg gtactcgctc gtcgtccagc cgcccacggg cgtcacgtac cagaacgaca
    13381 gcttcaagcg ccgtgtccac ctttcgcgcc tcaaggacgc ctcgggcaac gcgatggcgg
    13441 actacgaccg ggacttccgc gtctacgatc tcaacccgcc gcacgtcgac gtcgcgttcc
    13501 cggcgggggc gccgacgggc cagctcgtcg gcggatcggc gtacacgctc gcaccggtgc
    13561 tctcgagcct cgacgatctg ccgcagggcg acgtcgaccg cgtcgagtac ttcctcgcgt
    13621 cgtcgggcga cccgtcggcg ccggcctcga cacccgcgtt cacggcgcgg acctcgccgt
    13681 tcgggtggac gttcgtcgcg gcctacgtcg ggaacggcgt aaacccgcgc cccttcccgg
    13741 tgtgggtgaa agcgaccgac acgtccacga acgcgagcaa cgtcgtgaag cttgcgatgg
    13801 cggtcctgcc gaacgcgccg cccgtgatca cgtccatcgc cccggccgca acggcgcccg
    13861 tcgcggggac gttctacgcg ggctccgccc tcgcggcgac cgtcgccggc gtcgcggatc
    13921 ccgacggagc gtccgtcacg ctgacggccg agctccgtaa ggacaacgtg ggcagcccga
    13981 acgacccggc cgacctcgtc gcgagccttc cgtcgcaggc gctcgcgaag ccggcggcgg
    14041 ggtgggcggc gctgccgccg ccggtcttcg cgtcggcgat tccgatcacg acggcggagg
    14101 gcacgtcgct cttcttccgc gtgaaggcga cggattccct cggcgcatcg gtcacggccg
    14161 aatccctccg attcgccgtg gcgcacgacc cgaatgcgcc gagcgtcgac tccttcgtcg
    14221 cacggctctc cggctcggcg tcgccgtcct cgcagttctt catcggccag aagttcgtcc
    14281 tcgagttccg agcgcgcgat tccgagaccg ccctccgcac gacctcgatc gcgctgtccg
    14341 gtgtcttccc gtcgccgctg ctcgcggcgc tcgtcggcgg gacgacgaac ctctaccgga
    14401 cggtcgagct gaccgttccg gccacggtgc ccccgggcgg cctcgcgatc gccgcgaccg
    14461 cctcggccac cgactggggc gggaacacgg ggacgtcgcc gctggcgttc aacgtctcgc
    14521 ccacgcccga cccgtacgcg ccggccgcga cgtggctgac gccgtgggag agcggagcgt
    14581 ggccgtccgg gtacacgtcc accgtgtcgg cgcagggggc cgcgatgctc ctgcgcgtca
    14641 aggtctcgga tctcgaccgc gtcagcaacg tggacgtgcc gggcacgatc gcgagcgtcc
    14701 agttcaaggg cccggcggac gccgcgggca cgctcgccac ggctttcgtc gacggcgttc
    14761 tcgtcgcggg aacgggcggt ccaggcaccg ggatctacga agcgctctgg cgcgtcccga
    14821 acggcgtcgc ctccgggacg cagctgccgt tccaggtgcg cgtcgtggac gcgggcgcga
    14881 atgcgacggt cgccgacgtc cgtctccgcg ccgtgccgcc gcgcaaagtc tacgaggccg
    14941 cccaggtcgc cgcgctgccg gccgacacga tgctcggcgc gggtggcgac gcggcggggc
    15001 ccgtgttcct cctcgacgga acgacgttga gcctctaccc gcagacggct cccgccgtgc
    15061 gcgcgctcgc gtcgatgttc gtttacgcag gaggcgtcgc ggcgggcgcg acgttcacgc
    15121 cgacggcgag cgtcctcacg gcgcccgagg tcacgtcgta cgcgtcgtcc gtcctctaca
    15181 acccgctgga gctgaccgtg accgacacgt tcggcctcgg gcacggggct cgtgtggacg
    15241 tgtccgcgaa gggccttctc gggtccacgc cgacgcaggc gatggtcctg ccgggacaga
    15301 cgggctcgca gcagaacgcc ggcggctcgc acggcgggtc cggcgggccg ggctcgccgc
    15361 tcggctggac gcggaccgac ctgtacgcat cgggctcggt cttcgactcg gtgaaggacc
    15421 ctgcgctgcc gggcggcggc gccggctatg cgaccggccc gttcggcgcg acggccgcag
    15481 gcgggacggg cggcggcgtc gtgcggct
//
LOCUS       DAOMFY010000065        15499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1176_length_15499_cov_9.442567, whole genome shotgun sequence.
ACCESSION   DAOMFY010000065 DAOMFY010000000
VERSION     DAOMFY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15499
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1176_length_15499_cov_9.442567"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLB01_07390"
     CDS             complement(<1..941)
                     /locus_tag="PLB01_07390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ77163.1"
                     /translation="MLTIEAGTMIGPFEVVSLLGSGGMGDVYRGRDTRLGREVALKVL
                     PAHLADDKEGLVRFAHEARAASALNHPNIVTIYDIGRTDGAPFLAMEFITGRTMREIL
                     KDGPLPLRRLLDVAAQLTDGLASAHGAGIVHRDLKPENLMLSSDGFLKILDFGIAKLS
                     PAAAGGAATPALTSTGLVVGTPGYMSPEQASGRAVDFRSDQFSVGLLLYEMATGRRAF
                     ERPTPVETLTAIIREEPEPLAVAAPRLAAPVRWVIERCLAKDPEERYASTRDLAREWQ
                     QLERNADILATDTSLQTAQTARHEPVATTRTRAGAGPS"
     gene            complement(954..1433)
                     /locus_tag="PLB01_07395"
     CDS             complement(954..1433)
                     /locus_tag="PLB01_07395"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HQQ77164.1"
                     /translation="MISSYGQASAPTSQGFAEFGSLSVARDFPLTSGFAWGVELTPLF
                     LVSLTRVDLPDRPRESRAAVAAAPFLAWDAFPRAAVGLRLEASVGLLWAFSPVPAEGS
                     RFNFLDILGGRVRVRLGGGAALSVGVRRTHVSNLGTVGPDNPGLSFYAGALSLDLAR"
     gene            complement(1637..2971)
                     /locus_tag="PLB01_07400"
     CDS             complement(1637..2971)
                     /locus_tag="PLB01_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ77165.1"
                     /translation="MRIRLALFAAVLSLPLAAAESGLTVKCGTLLDPGSRVSKTNVEI
                     FFEGGKVARVEAASAAAAHPPSSPSKKSSSESPSPSSVLDLSSYTCLPGLIDAHTHLL
                     LQGDATSEEYDEQVLKESTPYRALRAAAAGRIALDHGFTTVRDLGSEGAGFTDVDLRK
                     AFDRGVVPGPRVFAAGPAMTPTGRYPLLGFSWERKMPDGVLKCDGADECRKAVRYQAQ
                     YGVDWIKVYADGSYYRTPDGGWASIPNFTQDEMNAIVDETHRLRKKVAAHSMTPTGHR
                     IALQAGVDSLEHGDVLDDETVKTMVAKKVAYCPTITVADFVKGPRSKTNPIWDQLWAA
                     SQDSFRRAYKAGAPIAFGTDAGGFAWDKRNQAEEFSFMVAWGMTPWDALRTATTVAAD
                     LLGPHKDGKLGCLADGCVADLVAVKGDPLTDIKVMEKTVAVISRGKVVKKPD"
     gene            3012..3572
                     /locus_tag="PLB01_07405"
     CDS             3012..3572
                     /locus_tag="PLB01_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ77166.1"
                     /translation="MSRAEELRQARERMEAVRAGIYEAVEGRSDAELLCPPADGGWSA
                     AEVLDHIGTAERTLVKALTKHEKGEPTRVPKYAWWYRLPMSPVFWKIKFRAPKLVRPR
                     PRSEVNPREVVAGLKESRAALLAIADRMGEERFAEMVFPHFLLGRFSGVDWFTFLARH
                     EGKHLGQIRRVLAGPKVLRCGGAKTA"
     gene            complement(3569..4396)
                     /locus_tag="PLB01_07410"
     CDS             complement(3569..4396)
                     /locus_tag="PLB01_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQQ77167.1"
                     /translation="MRIPDIEPELLAKACDGDLAALDEVVVAVQPGVFNLAVRILGNR
                     EDARDACQEILLKIVTRLSSFRGESAFGTWVWRVAHNHLMTAATRTRETVSLEEIGTR
                     LEAGLAFGAAAGRGTLLTPEEKAEARDVAVNCTQGMLMALDRDQRLAYVLDVVFGLSS
                     EEAAAVAEIEPAAHRKRLSRARARLEGFLKSTCGLVSPDVPCRCDRQLAALRAHARAE
                     PLAPRVTRQEASAAYDALRTLYETTGVFRAHPTYAPPGALVEAIRAALGAVRPAPES"
     gene            complement(4406..5143)
                     /locus_tag="PLB01_07415"
     CDS             complement(4406..5143)
                     /locus_tag="PLB01_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77168.1"
                     /translation="MPSPRAATLLTAGLALSVAAPARPSPAPAETAATPVVTIVRVKK
                     PWYAPRSAVVRKMRETIPQYQSIPGLAYKIYSLTADGRYGGLYLWTDRASAEAWFSPE
                     WFVRVQRERKTPGEVRFFDAPIVLDNTPGGTPANASSTAVASLVLLPVPAGASREKMI
                     QNFEAAVPTYRKVPGLLRKAFVLDGAGRFGGVDLWVSRVDAERHYSRAWHDEARQRYG
                     QDAEIEWFDAPILAPSTLATNRVALAE"
     gene            complement(5195..5824)
                     /locus_tag="PLB01_07420"
     CDS             complement(5195..5824)
                     /locus_tag="PLB01_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HQQ77169.1"
                     /translation="MAAAWAEAWRDPRFRLRLALTVPALVAVLALLARFLTFVEARPG
                     AVLTDPVLALLAPRDLTWLTFSLIYLGLAFGVARLAADPRALVVALQAYVLMVIFRMA
                     AMWVTPLEAPPGMIPLHDPLVRLFGPGKLLTKDLFFSGHTSTLFLLALAVPGRRSKAL
                     FLFCTAAVGISVLAQHVHYTIDVLAAPFFAYASFRIARLFDGPRSQGPA"
     gene            complement(5835..7271)
                     /locus_tag="PLB01_07425"
     CDS             complement(5835..7271)
                     /locus_tag="PLB01_07425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQQ77170.1"
                     /translation="MLKQQARTIAALLYAADISVTLATLPVAYLLRSEIVPHFVKSLT
                     ALYDFNMYLVLVGPIVLIWTALFFLGGAYRSRRTEALKDEVALVARTALFGSVLLALV
                     VFGARWDFISRPFLAAFFVVNLLLLVAERVTVRLVARRVRALGFNFRTVVLVGDTPGA
                     ASMAKLIRDHPWWGLKLLGLIREKPLEPGETTTSNGVPYLGTLADFPRVFTEFSVDEV
                     LLAVDRGDLPKMEDLFLLCEEMGVKTRLVLDFFPHVLARVELEELDGTPLLTFSTTPD
                     DSAAMMAKRTVDVLLSLVLGAVAVVPVTLAALLIKLTSKGPALFRQTRVGLNGRPFTL
                     VKLRTMVEDAEQRLAEVAHLNEHEGPVFKSAHDPRLTPFGRLIRRFSLDEIPQLWNVL
                     RGEMSLVGPRPPLPEEVARYERWQRRRLSMKPGVTGLWQVSGRNEIPRFEEWTNLDLA
                     YIDNWSLALDAKILLRTIPTVLSGRGAR"
     gene            complement(7333..8433)
                     /locus_tag="PLB01_07430"
     CDS             complement(7333..8433)
                     /locus_tag="PLB01_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HQQ77171.1"
                     /translation="MRIAILGTRGVPAAYGGFETFAEELSARLAARGHDVTVYARHGG
                     TAGEVALHRGARVVFLPTVRHKYFETVVHTALSGLHAAAQGYDAVLVCNGVNALACRF
                     ARLLGAGTRVVLNVDGLERNRRKWNAAGRLAYAVSERLSCVLPDVLVTDARAIQAYYR
                     DTYGQDSLYVPYGSDLAEPSGTAALRGLGLSPGGYVLYVSRFEPENNADAVVRAYRGV
                     PGAAPLVMLGGAPYADAFVAAVKAEAAGDPRVLLPGALYGEGYRELLANAAVYVHATE
                     VGGTHPALLEAMGFGRPLVVHDTAENRETAGTAALYADAVRPETLTRAIQALLADPEL
                     RRHSGEAARRRARKHYRWDEVTDAYEKALSDA"
     gene            complement(8430..9455)
                     /locus_tag="PLB01_07435"
     CDS             complement(8430..9455)
                     /locus_tag="PLB01_07435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ77172.1"
                     /translation="MTARVLVSLVTHNESRDAERLVPSLFAQSFRDLAVSAVDNASED
                     GTRATFAAFEKIAPVPLEVFPSRENLGYTGGHNRSIAKAVERGIPWVLVLNTDVVLAP
                     GFVATLLAEAGRPEHARVAAFTGKILRAEGPDLTPTSVIDSVGIRMTPNGRHFDVGAG
                     EPDDGRFDRPAEIFGASGCAVLFRTEALADVRISTGFFDDDFFVYREDVDLAWRLRGR
                     GWAARCVPSAVAWHRRRNLPERRREMSPLANLHSVKNRFLLRINNAGKAHLRATFFRT
                     FLRDALVVGGCLTVERTSFEALRWLAENRARLLEKRAEIQGRRTVSDADLARWFTDDP
                     RGARIEA"
     gene            complement(9452..10396)
                     /locus_tag="PLB01_07440"
     CDS             complement(9452..10396)
                     /locus_tag="PLB01_07440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ77173.1"
                     /translation="MTAPRVLVAIVSWNSAAHLPAAIRSVPSGVPVVVVDNASTDGSA
                     EAARAAGARVVEAGANLGFGPACNLAAKEGGPSETIFFLNPDAALVDGTSALAALLEA
                     LDVDPGVAAVAPRLEGAGQELFQLRRLPHAGSIVREAFLFDRLFPSNPWRLRDRYLDR
                     PRNEGFDVEQPAAAALLVRRADFEAVGGFDPAFAPAWFEDVDLCAKLLERRHRIRYVP
                     SAVATHVGGVAMDSLAWSDFRPLYVRNERRYLARHASLPLRALAWAATFSGALLRLVL
                     IPFVGGDHPRAESARAQVRVIRGLLGLGWRSALLPGAA"
     gene            10469..11755
                     /gene="serS"
                     /locus_tag="PLB01_07445"
     CDS             10469..11755
                     /gene="serS"
                     /locus_tag="PLB01_07445"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQQ77174.1"
                     /translation="MISRDFLRREFERLPELYRGRNLPPGALEAWRAIDVERREAITK
                     LEAARAEKNAVSEKVGALRRAKQDATELQERSKELAAAILSFEESLKALDARFAPIEE
                     TLPNVPHASVPEGADETANVVVKTWGTPTTFDFAPQAHWDLGPALGILDFERAAKITG
                     SRFTVLRGGASLLSRALIQFFLDVHTREHGYTELLPPFIVNAASLFGTGQLPKFEADL
                     FRLEGTDWYLAPTAEVPVTNMHRDEILPESALPISYCAYTPCFRAEAGAAGKDTRGMI
                     RQHQFDKVELVKFAKAEHSYEALETLTRDAESILEKLELPYRRIALCRGDLGFASAKT
                     FDLEVWLPGQDAYKEISSCSNFEDFQARRAAIRVRVESGGKPRNELVHTLNGSGLAVG
                     RTLVAILENFQRKDGSVAIPKALQPYCGGKTEIVKA"
     gene            complement(11760..12110)
                     /locus_tag="PLB01_07450"
     CDS             complement(11760..12110)
                     /locus_tag="PLB01_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77175.1"
                     /translation="MPDADLIDLFVAPLNRAGLTYMVTGSVASMLYAEPRFTADVDVV
                     VRVTSPGELRKAFSGPEYYCPPAEEGGSSKHVRDVRALIAAQGEKLDYATLERFAEER
                     GVADLLAEIRSARA"
     gene            complement(12103..12330)
                     /locus_tag="PLB01_07455"
     CDS             complement(12103..12330)
                     /locus_tag="PLB01_07455"
                     /inference="COORDINATES: protein motif:HMM:NF040165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77176.1"
                     /translation="MASAASETTHRLPDDAPDPEQMRRFAAMTPDERWAVAQALQRTA
                     REVKRAGLRAEHPDWTDEQVLEEVRRQFLRA"
     gene            12532..13152
                     /locus_tag="PLB01_07460"
     CDS             12532..13152
                     /locus_tag="PLB01_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ77177.1"
                     /translation="MCPEVTEHSVEQRLLDAAIQLFTERGYTATSVRELVERAGVTKP
                     ALYYHFASKEDLYLAILSDLARYADEVIARSRVRSGPVRERLEKFLVAMFEAFEEKKS
                     AVRLINAVFWGPAQGAPPFDFATFHNKLLVVLREIVDEGIAAGELRAANPADMTLALM
                     GVLSFTMDLTLARPELGVGKDGLRRALDLLFAGLAAPGPAFKETPR"
     gene            13149..14255
                     /locus_tag="PLB01_07465"
     CDS             13149..14255
                     /locus_tag="PLB01_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ77178.1"
                     /translation="MTRSNAALLSSVLALAAAGCSKPVAGRAAPGAGRPPVAVETVKV
                     ATADLEQTVDVVGTLAARSEADVRTEYSGTVAEVYVTQWVRVKAGTPLARLDTREADA
                     AVAAARAAALQAEVAVQRAARELERSAKLKQAGLATQQGLDEARTADEAARAGEAAAK
                     AQLAMAETRRTKAVLRAPIDGVVATRNVNVGDFVENMGNPPPMFRIVDNRLLELTASV
                     PSARMAELRPGQPFAFSSDAFPGREFAGRVSFINPAADEASRTVKVKTEVPNPDESLK
                     AGLFVKGRIVTGRRSSILVVPRSALVSWDTAARSAGVFVVEGGAAKRLLVETGAASGD
                     RVEIVKGLAAGQDVVTRGGFSLRDGDRVQTSAKA"
     gene            14259..>15499
                     /locus_tag="PLB01_07470"
     CDS             14259..>15499
                     /locus_tag="PLB01_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQQ77179.1"
                     /translation="MFLSNLSIKKPVFATVLMLALVTLGVFSYRRLAIDMFPDVEIPV
                     LSIFTRFPGASPETVEREVTKKLEQAVNPISGVKHVFSTSREGLSTVIIEFNLEVKIN
                     DAAQDARAKISAIRNDLPQGIEEPVIQKLDIGGMAVVALAVRSDAMTPRELTTLVDRK
                     VKRRLENLPGVGKVDLVGGSKREVTVDVDRARLDALGLGVDEVVAGLSGENVNTPLGR
                     LDGGGQELPVRVSGKPKEVPGFRSMVVATRGGVAVTLGEVADVKDGVEERRKLALVNG
                     VPAVALDVFKQSKANTVGVVEAVTKEIERLQTELPPSVEIQLVRDGSVMIKESVHDVT
                     NTLILGGVLTVLIVFCFLNSWRSTVITGLTLPISVISSFIAMYFLGMTLNVMTLMALS
                     LAIGLLIDDAIVVRENIVRHL"
BASE COUNT         2405 a         5214 c         5749 g         2131 t
ORIGIN      
        1 gacggcccgg cccccgcgcg cgtgcgcgtc gtcgcgacgg gctcgtggcg cgcggtctgg
       61 gccgtctgga gggacgtgtc cgtcgcgagt atgtcggcgt tgcgctcgag ctgctgccac
      121 tcgcgcgcga ggtcgcgcgt cgacgcgtaa cgctcctcgg ggtccttcgc gaggcagcgc
      181 tcgatgaccc accgcacggg ggccgcgagc cgcggcgcgg cgacggcgag gggctcgggc
      241 tcctcccgga tgatcgccgt gagcgtctcg acgggcgtcg ggcgctcgaa ggcgcgccgg
      301 cccgtcgcca tctcgtagag gaggagcccg acggagaact ggtcggagcg gaaatccacg
      361 gcgcggcccg acgcctgctc gggcgacatg tagcccggcg tcccgacgac gagtcccgtg
      421 ctcgtgagcg ccggcgtcgc ggcgccgccc gcggccgccg gagagagctt cgcgatcccg
      481 aagtcgagga tcttgaggaa cccgtccgag gagagcatga ggttctcggg cttgaggtcc
      541 cgatggacga taccggcgcc gtgcgcgctc gcgagcccgt ccgtgagctg cgcggcgacg
      601 tcgagcagcc ggcgcagcgg cagggggccg tccttgagga tctcgcgcat cgtccggccg
      661 gtgatgaact ccatcgcgag gaagggcgcg ccgtcggtcc tcccgatgtc gtagatcgtg
      721 acgatgttcg ggtggttgag cgcggaggcc gcgcgggcct cgtgggcgaa gcggacgagg
      781 ccctccttgt cgtcggcgag gtgcgccggg aggaccttca gggcgacctc gcgcccgagg
      841 cgcgtgtcgc ggccgcggta aacgtcgccc atgccccccg agccgaggag cgacacgacc
      901 tcgaacggcc cgatcatggt cccggcctcg atggtcaaca cgggaaggat aagtcagcgc
      961 gcgagatcga gagagagcgc ccccgcgtag aagctcaggc ccgggttgtc gggaccgacc
     1021 gtgccgaggt tggagacgtg cgtccggcgg acgccgaccg acagcgcggc gccgccgccg
     1081 agacggacgc gcacgcgtcc cccgaggatg tcgaggaaat tgaagcgcga gccctccgcg
     1141 ggcacgggcg agaacgccca gaggaggccc accgaggcct cgaggcgaag gccgaccgcg
     1201 gcgcggggga acgcgtccca cgcgaggaac ggcgcggcgg ccaccgccgc gcggctctcc
     1261 cgcggccggt cgggcaggtc gactcgcgtg aggctcacga gaaagagcgg cgtcagctcc
     1321 acgccccagg cgaagcccga agtgagcggg aagtcgcgcg cgacggagag cgacccgaac
     1381 tccgcgaagc cctgagacgt cggcgcggac gcctggccgt aggacgagat cacgtgccac
     1441 acgggcggcg cgggcggcgc ggcgggcgat ggcgtttgaa gattttcttc gagtggcaag
     1501 gagccggagg cagattgcga ggcgggcgcc tcgggcgccg gcggcgcggc agcccgggcc
     1561 gttttacctt ctaagaagaa aaccagcgtt gcggccagct gaatggcggc tcgcagcggt
     1621 cgggtctttg tccccctcaa tccggtttct tgacgacctt gccgcgcgag atcaccgcga
     1681 ccgtcttctc catcaccttg atgtccgtga gcgggtcgcc cttcaccgcg acgaggtccg
     1741 cgacgcagcc gtcggcgagg cagccgagct tgccgtcctt gtgcggtccg aggaggtccg
     1801 cggccacggt cgtcgccgtg cgcagcgcgt cccacggcgt cattccccac gcgaccatga
     1861 acgagaactc ctcggcctgg ttgcgcttgt cccaggcgaa gccgccggcg tccgttccga
     1921 acgcgatcgg ggctccggcc ttgtacgcgc gccggaacga gtcctgcgac gcggcccaga
     1981 gctggtccca gatcgggttc gtcttgctgc gcgggccctt cacgaagtcc gcgacggtga
     2041 tcgtcgggca gtaggcgacc ttcttcgcga ccatcgtctt gacggtctcg tcgtcgagca
     2101 cgtcgccgtg ctcgagcgag tcgacgcccg cctggagcgc gatgcgatgg ccggtcggcg
     2161 tcatcgagtg cgccgcgacc ttcttccgga ggcggtgcgt ctcgtccacg atcgcgttca
     2221 tctcgtcctg cgtgaagttc gggatcgagg cccagccgcc gtccggcgtc cggtagtacg
     2281 agccgtcggc gtagaccttg atccagtcga cgccgtactg cgcctgatag cgcacggcct
     2341 tgcggcattc gtcggcgccg tcgcacttga ggacgccgtc gggcatcttg cgctcccacg
     2401 agaagccgag gagcgggtaa cggccggtcg gcgtcatggc cggcccggcg gcgaagacgc
     2461 gcggcccggg cacgacgccg cggtcgaacg ccttcctgag gtcgacgtcc gtgaagcccg
     2521 cgccctccga gccgaggtcg cgcacggtcg tgaacccgtg gtcgagcgcg atgcgcccgg
     2581 ccgccgcggc gcggagcgcg cggtagggcg tcgactcctt gaggacctgc tcgtcgtatt
     2641 cctccgaggt cgcgtcgccc tgcagcagga ggtgcgtgtg cgcgtcgatg agcccgggga
     2701 gacaggtgta ggaggagaga tcgagaacgg acgaggggga gggagattcg gaggaagatt
     2761 tcttagaagg tgaagagggc ggatgcgcag cggcggcgct cgcggcctcg accctcgcca
     2821 cctttccacc ttcgaagaaa atctccacat tcgtcttcga gacacggctg cccggatcga
     2881 gaagggtccc gcatttcacc gtcaagccgg attcggctgc ggcgagcggc aggctcagca
     2941 cggcggcgaa gagcgcgagg cggattcgca tttgacggag gcccccttcc cgagcaggat
     3001 gaggggggat tatgagccgc gcggaggaac ttcgtcaggc ccgcgagcgc atggaggcgg
     3061 tgcgcgccgg gatctacgag gccgtcgaag ggcggtccga cgccgagctc ctctgcccgc
     3121 ccgcggacgg cggctggtcg gccgcggaag tgctggacca catcggcacg gccgagcgca
     3181 cgctcgtcaa ggcgctcacg aagcacgaga agggcgagcc cacgcgcgtg ccgaagtacg
     3241 cgtggtggta ccgcctcccg atgtcgccgg tgttctggaa gatcaagttc cgcgcgccga
     3301 agctcgtgcg gccgcgcccg cgctccgagg tgaacccgcg cgaagtcgtc gcaggcctca
     3361 aggagagccg cgccgccctc ctcgcgatcg cggatcggat gggcgaggag cgcttcgcgg
     3421 agatggtctt cccgcacttc ctcctcgggc gcttcagcgg cgtcgactgg ttcacgttcc
     3481 tcgcgaggca cgagggaaag cacctcggcc agatccggcg cgtcctcgcg gggcccaagg
     3541 tcctgcgctg cggcggcgcg aagacggcct agctctccgg cgccggccgg accgctccga
     3601 gggcggcgcg gatcgcctcg acgagagctc ccggaggcgc gtaggtcggg tgcgcccgga
     3661 agacgcccgt tgtctcgtac agggtccgca gcgcgtcgta ggcggccgac gcttcctggc
     3721 gcgtgacgcg tggagcgagg ggctcggcgc gcgcgtgggc gcggagagcg gcgagttgcc
     3781 ggtcgcagcg gcaaggcacg tccgggctca cgaggccgca cgtgctcttc aggaagccct
     3841 cgagccgtgc acgcgcgcgg gacagccgct tgcggtgcgc cgcgggctct atctccgcga
     3901 ccgcggcggc ctcctccgac gagagtccga acacgacgtc gagaacatag gcgagccgct
     3961 ggtcgcggtc gagtgccatc agcatcccct gagtgcagtt cacggcgacg tcgcgcgcct
     4021 ccgctttctc ctcgggggtc aggagggtgc cgcgtcccgc cgcagcgccg aaggcgagac
     4081 ccgcctcgag acgcgtgccg atctcctcga gcgacacggt ctcccgcgtc cgggtggccg
     4141 ccgtcatgag gtggttgtgc gcgacgcgcc agacccacgt gccgaacgcg gactccccgc
     4201 ggaagctgga gagacgcgtg acaatcttga ggagaatctc ctgacacgcg tcgcgcgcgt
     4261 cctcgcggtt tccaaggatg cgcacggcga ggttgaagac accaggctgc acggcgacga
     4321 cgacctcgtc gagcgccgcc aggtccccgt cgcaggcctt cgcaaggagc tcgggttcga
     4381 tgtcggggat gcgcatcgac ggagtctatt cggcgagcgc gacgcgattc gtcgcgagcg
     4441 tggagggggc caggatcggc gcgtcgaacc actcgatctc cgcatcctgg ccgtaccgct
     4501 ggcgcgcctc gtcgtgccag gcgcggctgt agtgacgttc cgcgtccacg cggctcaccc
     4561 agaggtccac gccgccgaag cggccagccc cgtctaggac gaaggccttg cgcaacaggc
     4621 cgggcacctt tcgatacgtg gggaccgcgg cctcgaagtt ctggatcatc ttctcgcgag
     4681 acgccccggc cggaaccggg agaagcacga gcgaggcgac ggccgtggac gaagcgttcg
     4741 ccggagtccc tcccggcgtg ttgtcgagga cgatgggtgc gtcgaagaag cgcacttctc
     4801 cgggcgtctt tcgctcgcgc tggacccgca cgaaccactc ggggctgaac cacgcctcgg
     4861 cgctcgcgcg atcggtccag aggtacaggc ccccgtaacg gccgtcggcg gtgagggagt
     4921 agatcttgta ggcgagaccc gggatcgact ggtactgggg aatggtctcc cgcatcttcc
     4981 ggacgacggc cgagcgcggc gcgtaccagg gcttcttcac ccggacgatc gtgacgaccg
     5041 gagtcgcagc ggtttcggct ggcgccgggg acggccgggc aggggcagcg acggagagtg
     5101 ccaggccggc agtcagaagg gtcgcggcgc gggggctcgg catggggtcc tccttcgcgg
     5161 ggcgcggtcg cgcctcgcaa aggtttggac ccggtcaggc cggtccttgt gaccggggcc
     5221 cgtcgaaaag ccgggcgatc ctgaagctcg cgtacgcgaa gaacggggcc gcgaggacgt
     5281 cgatcgtgta gtgaacgtgc tgcgcgagga cggagatccc caccgcggcc gtgcagaaga
     5341 gaaagagcgc cttcgaccgc cggcccggca cggcgagggc cagcaggaac agcgtggacg
     5401 tgtggcccga gaagaagagg tccttcgtga ggagcttccc cggcccgaag aggcgcacga
     5461 gcgggtcgtg gagcgggatc attccgggcg gcgcctcgag cggcgtgacc cacatggcgg
     5521 ccatccggaa gatcaccatg aggacgtagg cctggagcgc gacgacgagc gctcgcggat
     5581 cggccgcgag gcgcgcgacg ccgaacgcga ggccgaggta gatcagcgag aacgtcagcc
     5641 acgtcaggtc gcgcggtgcg aggagcgcga gcaccgggtc ggtcaggacg gcgcccggcc
     5701 gcgcctcgac gaacgtgagg aagcgcgcga ggagcgcgag gacggcgacg agcgcgggaa
     5761 cggtgagggc gagacgcagc cggaagcgcg ggtcccgcca ggcctcggcc caggccgcgg
     5821 ccaccgccgt ccgcctagcg cgcgccgcgg ccggagagga ccgtcgggat cgtgcgcagc
     5881 aggatcttcg cgtcgagggc gaggctccag ttgtcgatgt aggcgaggtc gaggttcgtc
     5941 cactcctcga agcggggaat ctcgttccgg ccggagacct gccacaggcc cgtgacgccc
     6001 ggcttcatgg agagccggcg gcgctgccat ctctcgtagc gcgcgacctc ctcggggagc
     6061 ggcgggcgcg gcccgacgag cgacatctcg ccgcgcagga cgttccagag ctgcgggatc
     6121 tcgtcgagcg agaagcggcg gatgagccgg ccgaacggcg tgaggcgcgg gtcgtgtgcg
     6181 gacttgaaga cggggccctc gtgctcgttg aggtgcgcga cctccgcgag gcgctgctcg
     6241 gcgtcctcga ccatcgtccg gagcttcacg agcgtgaacg gccgcccgtt caggccgacg
     6301 cgcgtctgcc ggaagagcgc ggggcccttc gacgtgagct tgatgaggag cgccgcgagc
     6361 gtgacgggga cgacggcgac cgctccgagg acgagcgaca gaaggacgtc gacggtgcgc
     6421 ttggccatca tcgcggcgct gtcgtcgggc gtcgtcgaga acgtgaggag cggcgtcccg
     6481 tccagctcct cgagctcgac gcgcgcgagg acgtgcggga agaagtcgag gacgagccgc
     6541 gtcttgacgc ccatctcctc gcacaggagg aagaggtcct ccatcttcgg gaggtcgccc
     6601 cggtccacgg cgaggaggac ctcgtcgacg ctgaactccg tgaagacgcg ggggaagtcc
     6661 gcgagcgtcc cgaggtaggg cacgccgttc gacgtcgtcg tctcgccggg ctcgaggggc
     6721 ttctcgcgga tgaggccgag gagcttgagg ccccaccacg ggtggtcgcg gatgagtttc
     6781 gccatcgagg ccgcgcccgg cgtgtcgccc acgaggacca ccgtccggaa gttgaagccg
     6841 agggcgcgca cgcgccgcgc gacgaggcgc accgtgacgc gctcggcgac gaggaggagg
     6901 aggttcacga cgaagaacgc ggcgaggaag gggcgcgaga tgaagtccca gcgcgcgccg
     6961 aagacgacga gcgcgaggag caccgatccg aagagggccg tccgcgcgac gagcgcgacc
     7021 tcgtccttga gcgcctccgt tcggcggctg cggtacgccc cgcccaggaa gaacagggcc
     7081 gtccagatca ggacgatcgg cccaacgagg acgaggtaca tgttgaagtc gtaaagggcg
     7141 gtcaggctct tcacgaaatg cggaacgatc tcgctccgaa ggagatacgc caccggcagc
     7201 gtcgcgaggg tgacgctgat gtcggcggcg tacaggaggg ccgcaatcgt ccgggcctgt
     7261 tgcttcagca tcggggcgga ccccggcgag gggtcatggg gatgctagca agtgccgcga
     7321 aaacgcgctt cctcacgcgt ccgagagggc cttttcgtag gcgtccgtga cttcgtccca
     7381 ccggtaatgc ttccgggccc gccggcgggc ggcctccccg gagtgccggc ggagctcggg
     7441 gtcggcgagc agggcctgga tcgcccgggt cagcgtctcc gggcgcacgg cgtcggcgta
     7501 aagggctgcg gtcccggccg tttcacggtt ctcggcggtg tcgtggacga ccagaggccg
     7561 gccgaagccc atggcctcga ggagcgcggg atgcgtcccg ccgacctcgg tcgcgtggac
     7621 gtagacggcg gcgttcgcga ggagctcgcg gtagccctct ccgtacaggg cgccgggcag
     7681 gaggacgcgc gggtcccccg cggcctcggc cttcacggcc gcgacgaacg cgtcggcgta
     7741 cggcgccccg ccgagcatca cgaggggagc cgcgcccggc actccgcggt acgcgcgcac
     7801 gacggcgtcg gcgttgttct cgggctcgaa gcgcgagacg tagaggacgt agccgcccgg
     7861 tgaaaggccc aggcccctga gcgccgccgt gccggagggc tcggcgaggt cggagccgta
     7921 cgggacgtag agcgagtcct ggccgtacgt gtcgcggtag tacgcctgga tggcgcgcgc
     7981 gtccgtcacg agcacgtccg gcagcacgca gctcaggcgc tcggacacgg cgtacgcgag
     8041 gcggccggcg gcgttccact tccggcggtt gcgctcgagc ccgtccacgt tcaggacgac
     8101 gcgcgttccg gccccgagga ggcgcgcgaa gcggcacgcg agcgcgttga cgccgttgca
     8161 cacgaggacg gcgtcgtagc cctgcgcggc ggcatggagg cccgagaggg ccgtgtggac
     8221 gacggtctcg aagtacttgt gccgcaccgt cggcaggaac acgacgcggg cgccgcggtg
     8281 gagggcgacc tcgccggccg tcccgccgtg ccgcgcgtag acggtcacgt cgtgcccgcg
     8341 cgcggcgagg cgcgcggaga gctcctcggc gaacgtctcg aagccgccgt aggcggccgg
     8401 aacgccgcgc gtcccgagaa tcgcgatcct cacgcctcga tcctcgcgcc gcgcgggtcg
     8461 tccgtgaacc agcgcgcgag gtccgcgtcg gacaccgtgc ggcggccctg gatctcggcg
     8521 cgcttttcga ggaggcgcgc gcggttctcg gcgagccacc ggagcgcctc gaaggacgtg
     8581 cgctccaccg tcaggcagcc cccgacgacg agggcgtcgc ggaggaaggt ccggaagaac
     8641 gtcgcgcgga ggtgcgcctt cccggcgttg ttgatccgga ggaggaagcg gttcttgacg
     8701 gagtggaggt tcgcgagggg ggacatttcc cggcgccgct ccgggaggtt gcggcgccgg
     8761 tgccacgcca ccgcggaggg gacgcagcgc gcggcccagc cccgtccacg caggcgccac
     8821 gcgaggtcga cgtcctcgcg gtagacgaag aagtcgtcgt cgaagaagcc cgtggagatc
     8881 cgcacgtcgg cgagcgcctc cgtccggaac agcacggcgc agcccgaggc gccgaagatc
     8941 tcggcgggcc ggtcgaacct cccgtcgtcg ggctcgcccg cgccgacgtc gaagtggcgc
     9001 ccgttgggcg tcatgcggat tccgacgctg tcgatcacgg acgtcggcgt gaggtcgggc
     9061 ccctccgccc ggaggatctt cccggtgaac gccgcgacgc gggcgtgctc cggacgcccg
     9121 gcctccgcga gaagcgtcgc gacgaaaccg ggcgcgagga cgacgtcggt gttgaggacg
     9181 aggacccacg gaatcccccg ctcgacggcc ttcgcgatcg accggttgtg gccgcccgtg
     9241 tacccgaggt tctcgcgcga cgggaagacc tcgagcggaa cgggcgcgat cttctcgaac
     9301 gccgcgaacg tcgcgcgcgt cccgtcctcg gacgcattgt cgacggcgga gacggcgaga
     9361 tcgcggaacg actgcgcgaa gagcgagggg acgagccgct cggcgtcgcg ggactcgttg
     9421 tgcgtcacga gcgagacgag gacgcgcgcc gtcatgcggc ccccgggaga agggcggagc
     9481 gccagccgag accgaggagg ccgcggatca cccggacctg ggcgcgcgca ctctccgcgc
     9541 gcgggtggtc gccgccgacg aagggaatga gcacgagcct caggagcgcg cccgagaacg
     9601 tcgcggccca cgcgagggcg cggagaggaa gggacgcgtg ccgcgcgagg taacgccgct
     9661 cgttgcggac gtagagcggc cggaagtcgc tccacgcgag ggagtccatc gcgacgccgc
     9721 cgacgtgcgt cgcgacggcg gacgggacgt agcggatgcg gtggcggcgc tcgaggagct
     9781 tggcgcagag gtccacgtcc tcgaaccacg cgggagcgaa cgccgggtcg aagccgccca
     9841 cggcttcgaa gtccgcgcgc cgcacgagga gggcggcggc ggccggctgc tcgacgtcga
     9901 aaccctcgtt ccgcggccgg tcgaggtacc ggtcgcgaag ccgccagggg ttcgagggaa
     9961 agagacggtc gaacaggaac gcctcgcgga cgatcgagcc cgcgtgcggg aggcgccgga
    10021 gctggaagag ctcctgcccg gcgccctcga ggcgcggcgc cacggctgcg acgcccgggt
    10081 cgacgtcgag ggcctcgagg agagccgcga gcgcggaagt gccgtcgacg agcgccgcgt
    10141 ccgggttcag gaagaagatc gtctcggagg ggccgccctc cttcgccgcg aggttgcagg
    10201 ccgggccgaa cccgagattc gctccggcct cgacgacgcg cgcgcccgcg gcccgggcgg
    10261 cctccgccga cccgtccgtg gacgcgttgt cgacgacgac gacgggcacg ccggacggga
    10321 cggagcggat cgccgccggc aggtgcgcgg cggagttcca cgacacgatc gcgacgagga
    10381 cgcggggggc ggtcaaagcg gttcgatgat agtgcgcggg gcgtgctgct tgaatcccgg
    10441 cggcgcgccc gacagaatgc agggctgaat gatctcgaga gattttctta gaagggaatt
    10501 cgagcggctg cccgagctgt accgcggccg caacctgccg cccggcgcgc tggaggcgtg
    10561 gcgcgccatc gacgtcgagc gtcgcgaggc gatcacgaag ctggaggccg cgcgggccga
    10621 aaagaacgcg gtttccgaga aggtgggcgc gctccgccgg gccaagcagg acgccacgga
    10681 gctgcaggaa cgttccaagg aactcgcggc cgccattctc tccttcgaag aatccttgaa
    10741 ggccctggat gcgcggttcg ctcctatcga ggagacgctc ccgaacgtcc cgcacgcgtc
    10801 cgtccccgag ggcgccgacg agaccgcgaa cgtcgtcgtg aagacgtggg gtacgccgac
    10861 gacgttcgac ttcgcgccgc aggcccactg ggacctcggc ccggcgctcg gcatcctcga
    10921 cttcgagcgc gcggcgaaga tcacgggctc gcggttcacg gtgctcaggg gcggcgcgtc
    10981 gctgctgtcg cgcgcgctca tccagttctt cctcgacgtc cacacgcgcg agcacggcta
    11041 cacggagctc ctgccgccgt tcatcgtgaa cgcggcgtcg ctcttcggca cgggtcagct
    11101 cccgaagttc gaagcggacc tgttccggct cgagggcacg gactggtatc tcgcgccgac
    11161 ggcggaggtg cccgtcacga acatgcaccg cgacgagatc ctccccgaga gcgcgctccc
    11221 gatctcgtac tgcgcgtaca cgccgtgctt ccgcgccgag gcgggggccg ccggcaagga
    11281 cacgcgcggg atgatccgcc agcaccagtt cgacaaggtc gagctcgtga agttcgcgaa
    11341 ggccgagcat tcgtacgagg cgctcgagac gctcacgcga gacgccgagt cgatcctcga
    11401 gaagctcgag ctcccgtacc ggcggatcgc gctctgccgg ggcgacctcg ggtttgcgtc
    11461 cgcgaagacg ttcgacctcg aggtgtggct cccggggcag gacgcctaca aggagatctc
    11521 ctcctgttcg aacttcgagg acttccaggc gcgccgcgcc gcgatccgcg tgcgcgtgga
    11581 gtccggcggc aagccccgaa acgagctcgt ccacacgctg aacggctcgg gtctcgcggt
    11641 cgggcggacg ctcgtcgcga ttctcgagaa cttccagcgc aaggacggct ccgtcgcgat
    11701 cccgaaagcc ctccagccct actgcggcgg gaagacggag atcgtcaagg cttgaggggt
    11761 caagcccgcg cgctgcggat ctcggcgagg agatccgcga cacctcgctc ctccgcgaac
    11821 cgctcgagtg ttgcgtagtc cagcttctcg ccctgcgcgg cgatgagggc gcggacgtcg
    11881 cgcacgtgct tcgaggatcc gccctcctcg gcgggcggac agtagtactc cgggcccgag
    11941 aacgcctttc gaagctctcc tggagaggtc acgcgcacca cgacgtccac gtccgccgtg
    12001 aagcgcggtt cggcatagag catgctcgcg accgaacccg taaccatgta cgtgagtccc
    12061 gcccggttca gcggcgcgac gaagaggtcg atcaggtcgg cgtcaggcac gcaggaactg
    12121 ccggcgcact tcttcgagga cctgctcgtc ggtccagtcg ggatgctcgg cgcgcaaacc
    12181 ggcgcgcttg acctcgcgcg ccgtccgctg gagcgcctgt gcaaccgccc agcgctcgtc
    12241 cggcgtcatc gcggcgaagc gccgcatctg ctccggatcg ggagcgtcgt cgggcagccg
    12301 gtgtgtcgtc tcggacgcgg cggaagccat gagttcagtc tagcgcccgg tgaggaaggg
    12361 gccgacgaga tatgtccgat cgaacgtgaa gatcagaacg gtgtgcggct ggcggtcggt
    12421 gggcatagag caccttgatc acttgacgcc tccgggtgcc gactccagtt ttacttaccg
    12481 gtcggtagtt cctctcggga acgagcagac ctgggtctgg aggccgtcag gatgtgcccg
    12541 gaagtcactg aacactctgt agaacaacga cttctggacg cagccatcca actcttcacg
    12601 gaacgcgggt acaccgcgac ttccgtgcgg gagctcgtcg agcgggccgg ggtgacgaag
    12661 ccagccctct actaccactt cgcgagcaag gaggacctct acctcgcgat tctgagcgac
    12721 ctcgctcgct acgccgacga ggtgatcgcg aggagccgcg tgcggagcgg cccggtccgg
    12781 gaacgcctgg agaagttcct cgtcgcgatg ttcgaggcct tcgaggagaa gaagtcggcc
    12841 gtgcgcctca tcaacgcggt cttctggggg cccgcgcagg gcgctccgcc gttcgacttc
    12901 gcgacatttc acaacaagct cctcgtcgtc ctcagggaga tcgtcgacga ggggatcgcc
    12961 gccggcgagc tccgggccgc gaaccccgcg gacatgacgc tcgccctcat gggcgttctc
    13021 tcgttcacga tggacctcac gctcgcgcgg ccggagctcg gcgtcggcaa ggacggcctc
    13081 cgccgtgcgc tcgacctgct cttcgcgggc ctggccgcgc cgggccccgc cttcaaggag
    13141 accccccgat gacgcgttcg aacgccgccc tcctctccag tgttctcgct ctcgcggccg
    13201 ccggctgcag caagcccgtc gcgggaagag ccgctcccgg ggcgggccgg ccgcccgtcg
    13261 cggtcgaaac cgtgaaggtc gcgacggccg atctcgagca gacggtcgac gtcgtcggca
    13321 cgctcgcggc gcgcagcgag gccgacgtac ggacggagta cagcggaacc gtcgccgagg
    13381 tatacgtcac gcagtgggtg cgcgtgaagg ccggcacgcc cctcgcgcgg ctcgacacgc
    13441 gcgaggcgga cgcggccgtg gccgccgcgc gtgccgccgc gctccaggcc gaggtggccg
    13501 tgcagcgtgc cgcacgggag ctggaacgga gcgccaagct caagcaggcg ggcctcgcga
    13561 cgcagcaggg gctcgacgag gcgaggacgg ccgacgaggc cgcgcgggcg ggcgaagcgg
    13621 ccgcgaaggc ccagctcgcg atggccgaga cgcggaggac gaaggccgtc ctccgcgctc
    13681 cgatcgacgg cgtcgtcgcg acgcggaacg tcaacgtcgg ggacttcgtc gagaacatgg
    13741 ggaaccctcc cccgatgttc cgcatcgtcg acaaccggct tctcgagctg acggcgagcg
    13801 tgccgtcggc gcggatggcg gagctgaggc cgggccagcc gttcgccttc tcgagcgacg
    13861 cgttcccggg ccgggagttc gcgggccggg tctccttcat caacccggcg gcggacgagg
    13921 cgagccggac cgtgaaggtg aagaccgagg tgccgaaccc ggacgaatcg ctcaaggccg
    13981 ggctcttcgt caaggggcgg atcgtgaccg gcaggaggag ttcgatcctc gtcgtccccc
    14041 gctcggcgct cgtctcctgg gacacggcgg cgcgctcggc gggcgttttc gtcgtggagg
    14101 gaggcgccgc gaaacgcctc ctcgtcgaga cgggcgcggc gagcggcgac cgcgtggaaa
    14161 tcgtgaaggg cctcgccgcc ggtcaggacg tcgtgacgcg gggcggcttc agcctgcgcg
    14221 acggcgaccg cgtgcagacc tccgcgaagg cgtgagccat gttcctgtcg aatctctcca
    14281 tcaagaaacc ggtcttcgcg accgtgctca tgctcgccct cgtgacgctc ggcgtctttt
    14341 cgtaccggcg cctcgcgatc gacatgttcc cggacgtcga gatcccggtt ctctccatct
    14401 tcacgcggtt cccgggcgcg tcgcccgaga cggtcgagcg cgaggtcacg aagaagctcg
    14461 agcaggcggt caacccgatc tcgggcgtca agcacgtctt ctcgacctcg cgcgaggggc
    14521 tctcgacggt catcatcgag ttcaacctcg aagtgaagat caacgacgcg gcgcaggacg
    14581 cgcgcgcgaa gatctcggcg atccggaacg acctccctca ggggatcgaa gaacccgtca
    14641 tccagaagct cgacatcggc gggatggcgg tggtggcgct cgccgtccgt tccgacgcga
    14701 tgaccccgcg cgagctgacg acgctggtcg accgcaaggt caagcgccgc ctcgagaacc
    14761 tcccgggcgt gggcaaggtc gacctcgtgg gcgggagcaa gcgcgaggtg accgtcgacg
    14821 tcgaccgggc gcggctcgac gctctcggcc tcggcgtgga cgaagtcgtc gccgggctct
    14881 ccggcgagaa cgtgaacacg cccctcggcc ggctcgacgg cggcggccag gagctgccgg
    14941 tgcgcgtctc gggcaagccg aaagaggtgc cgggcttccg gtccatggtc gtcgcgacgc
    15001 gcggcggcgt cgccgtgacg ctcggggagg tcgccgacgt gaaggacggc gtcgaggagc
    15061 ggcgcaagct ggcgctcgtc aacggcgtgc cggccgtcgc gctcgacgtc ttcaagcaga
    15121 gcaaggcgaa cacggtcggc gtcgtcgaag ccgtcacgaa ggagatcgag cgcctccaga
    15181 ccgagctgcc cccgtccgtc gagatccagc tcgtccggga cggctccgtg atgatcaagg
    15241 agtccgtgca cgacgtcacg aacacgctga ttctcggcgg cgtcctcacc gtgctcatcg
    15301 tcttctgctt cctcaactcg tggcggtcga ccgtcatcac gggcctcacg ctcccgatct
    15361 ccgtcatctc gtcgttcatc gcgatgtact tcctcgggat gacgctcaac gtcatgaccc
    15421 tgatggcgct gtcgctcgcc atcggcctcc tgatcgacga cgcgatcgtc gtccgcgaga
    15481 acatcgtccg gcacctcga
//
LOCUS       DAOMFY010000066        15449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1179_length_15449_cov_7.896193, whole genome shotgun sequence.
ACCESSION   DAOMFY010000066 DAOMFY010000000
VERSION     DAOMFY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15449
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1179_length_15449_cov_7.896193"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..731
                     /locus_tag="PLB01_07475"
     CDS             246..731
                     /locus_tag="PLB01_07475"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HQQ77180.1"
                     /translation="MALVDMFLKLDGVEGESKDASGKGGWIQISGFRIGAVSPRDAFT
                     NQAKGAVQMSHLTIRAKVDKSTPKLFTKIKTNEKIPTAVLSCRKAGKEQFEYFSITLS
                     EALVVRVQAGSLESMGSDVVPECEFDLAYGKLEMKSMEQTSMGPTSGPVVFSFNLMSN
                     A"
     gene            938..1429
                     /locus_tag="PLB01_07480"
     CDS             938..1429
                     /locus_tag="PLB01_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HQQ77181.1"
                     /translation="MAVVDYFLKLDGIDGESKDDKYKDHIQLESWSWGETNASSGHVG
                     SGHGSGKVSAQDFHFVMKVNKATPKLILACAEGHHIPNATLIARKAGKGQQDFLKYKF
                     TDVFVSSFQTGGHAGADTIPTDQISLGYVKMEIEYKEQQKDGSLGGAVKTGYDFQANK
                     KLA"
     gene            complement(1490..2302)
                     /locus_tag="PLB01_07485"
     CDS             complement(1490..2302)
                     /locus_tag="PLB01_07485"
                     /inference="COORDINATES: protein motif:HMM:NF018699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HQQ77182.1"
                     /translation="MPTPRELYQAGQLQAAIQALTAEVRANPADGPTRIFLFELLSFA
                     GDWDRARKQIEVLGQAGPNEALAVQVYLANIEAERSRERLFSEGLHPHFLNEPPPYVD
                     RHLEAINRIREGNVAEARILLDRAEEERPALPGTLNGRPFADFRDCDDVTGPVLELIV
                     KDKYTWLPFEQIRRLEISPPSKLRDLVWARAKVEALDGTVGDVYVPALYAGTCRSADD
                     QLRLGRMTTWIPLAEDLSRGAGLKTFQVDEDDVAMFEARQVEFRPPGPAAVA"
     gene            2425..3534
                     /gene="tssA"
                     /locus_tag="PLB01_07490"
     CDS             2425..3534
                     /gene="tssA"
                     /locus_tag="PLB01_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HQQ77183.1"
                     /translation="MPSAPVFNLDALLAPIAGEKPCGENLQYSGLHDEIREARRADDD
                     VPQGDWVHELKVADWEKVAELAGESLASKTKDLQIVAWLSEAAVNMHGFPGLRDSLKL
                     ATGYLGNFWDALYPEIDEGNDLEARANAIAWMDRQAAEAVRKVPLTKRSGANYNFFQW
                     DEALQYDFPADTSKLSSEALAQLEEKRARAQAEGKITGEEVRKAKSETRRAFYEELFA
                     DVADCWSALKALDKEMDARFARQTPGLGNLTKSLDAIRTLLEKVVKEKRIEEPDPSDA
                     TEGAEGEAAAGGGGGPGGTIRARDQALAQLAQVADYFRRTEPHSPVSYLVQRAIAWGQ
                     MPLDAWLVDVIKDTNVLAGLRETLGLKDESGGTGT"
     gene            3546..4085
                     /gene="tssB"
                     /locus_tag="PLB01_07495"
     CDS             3546..4085
                     /gene="tssB"
                     /locus_tag="PLB01_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HQQ77184.1"
                     /translation="MPKKESIQQKLKRIRPPRVQITYDVEIGGAIEMKELPFVVGVLG
                     DFSGQPAEPLPRLKDRKLIEIDRDNFDQVLAAQAPRLALRVDNKLSDDGSKMNVEMKF
                     NNMTDFEPDRIVQQVEPLRKLVEARQKLRDILSKTDGNDKLEAMLTDLLKDPNAQKQL
                     GKQLGVVTDEEPKKEEPHE"
     gene            4078..5562
                     /gene="tssC"
                     /locus_tag="PLB01_07500"
     CDS             4078..5562
                     /gene="tssC"
                     /locus_tag="PLB01_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HQQ77185.1"
                     /translation="MSKNPEKKAESVVQEQEGGLLNKILESGRLALRADQVDNAKDML
                     AEFVQQALQGQVVLSKDLEHTINSRIAAIDKLLSAQLNEIMHHADFQKLEGSWRGLHH
                     LVFESETSTMLKIRVLNVSKKDLLKDLERAAEFDQSALFKKIYEEEYGTFGGAPFGAL
                     IGDYEFGNHPQDMSLLEKISGVAAAANAPFISAASPEMFGWQSYTELTEVRDLAKIFD
                     RTEYAKWRSFRDSEDSRYVGLTLPHTLMRLPYGKDTVPTESFDFEEDVDGKDHKKYLW
                     GNAAYSYGTRLTEAFALYNWTVAIRGVEGGGLVQGLPAHTFQTDEGDVALKCPTEIAI
                     TDRREKEFSDLGFISLVHCKNTDYAAFFGGQSAQKAKKYDTDFANANARLSTQLPYIF
                     AVSRFAHYLKAIMRDKIGSFASRADVEFFLNKWISNYITPDDTASPAVKAQFPLREAR
                     IEVAEVAGKPGVYKAIAFLRPHFQLDELSVSLRLVAELPAPTRK"
     gene            5626..6108
                     /gene="tssE"
                     /locus_tag="PLB01_07505"
     CDS             5626..6108
                     /gene="tssE"
                     /locus_tag="PLB01_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HQQ77186.1"
                     /translation="MSRIVDEIRINLSVLDRLVDFEPEVKAEPPTSRSKAIRQVKQAL
                     KRDLEWLLNTRRAFEVPTELPLLSDSLLAYGLPDFSNVSVKNSGDQHNLTLAVEAALR
                     RFEPRLEDVVVSVASASVLERAFRFRIEARLRVDPVPEPISFDTTLQIGSGNFAVKGD
                     "
     gene            6108..7964
                     /gene="tssF"
                     /locus_tag="PLB01_07510"
     CDS             6108..7964
                     /gene="tssF"
                     /locus_tag="PLB01_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HQQ77187.1"
                     /translation="MRSELLEYYERELSFLRQMGAEFADKYPKVASRLQLEPDKCEDP
                     HVERLLEAFAFLAARVRLKIDDEFPEVTESLLGILYPTFLAPVPSMSVAQFVLNPEQV
                     SLQTGQTIAKGSTLASAPVDGIPCRFRTAYPVTIWPVEVKAARFEQAPAVTIDGRDAR
                     TMLHLDLRILGGAALSELKEKTSDGEERPLSRLRFYLQGEGKVVHALYELLFNDALAV
                     ELRPQAAAPDSAAIVLGPEALRPVGFGDDETLLPTPDRLFRGYRVLQEYFAFPEKFLF
                     VDLDGLDRLQGRGFTDTLDVRILLGRPFAAERSVSAQTFRLHTTPIVNLFSQMAEPIR
                     VTHLSHEYRVIPNLRRPGAHEVWSVDSVTSTAADLETVRAYQPFFAFRHNTERTGHDA
                     FWHANRRPSERKDDDGTEVYLTLVDMNFDPSVPDTDTLTVHTTCSNRDLPARLPFGNP
                     DGDFQLEGPGVFTAIRSLVKPTASLRPPLRRGAHWRLISHLALNVLSLVEREGEKGPE
                     ALREILRLYDIADSSVTRQQIDGISRLSARRVVRPLRDIHPGFVRGLEVSIEFDEQNF
                     VGSGIYLFASVLERFLSLYASVNSFSQLVASTKQREGVMRRWPPRTGDQIVL"
     gene            7969..8961
                     /gene="tssG"
                     /locus_tag="PLB01_07515"
     CDS             7969..8961
                     /gene="tssG"
                     /locus_tag="PLB01_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HQQ77188.1"
                     /translation="MTERLRTRPEHFGFFQAVRLLSRLSPSRAPVGQDGPPAREIARF
                     RTRATLEFPPSEIFELRPDATDPDRPPEVTVAIGGTTGPLGVLPTPYTELLIERVRYK
                     DYALWEFLDLFNHRFLSLFHRAWQKYRFPVVYESGGDDPFVEGLFALIGLGTGSLRAR
                     QSVPDDALLHYAGLVAQRPHSSSALVGILRDYFGVPVANRQFVGQWLDLEPEDRSRLG
                     SRNSRLGVDLVCGDHVWNNQSRFRIRLGPLSLASFQKFLPRGNAWRPLNDLVRFLVGV
                     EYDFDVQPVLKKEEIPECHLRSGAAVPPMLGWTTWLKSAPAPADASDLILESMN"
     gene            9001..11730
                     /gene="tssH"
                     /locus_tag="PLB01_07520"
     CDS             9001..11730
                     /gene="tssH"
                     /locus_tag="PLB01_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HQQ77189.1"
                     /translation="MGANLKSLIGRLNDTCRNALEGAAGLCLSRTNYDVDVEHLLVKL
                     GETPDTDFHRILRRFEVDESRLAKDLVRSLDRLKTGNTRTPSLSPRLPKLVEEAWLVG
                     SIDFGASRVRSGHILLALLEKDEFARLAREISKELALISVEALRKEFDKIVSGSVEDR
                     DAALLGEAPAGDAAGPSGVPGRTKALDQYTVDLTARARSGHIDPILGRDFEIRQVIDI
                     LTRRRQNNPILTGEAGVGKTAVVEGFALRIAQGDVPGPLKNVAVRTLDLGLLQAGAGI
                     KGEFENRLKTVIEEVKSSPQPIIVFIDEAHTMIGAGGQAGQNDAANLLKPALARGELR
                     TIAATTWAEYKKYFEKDAALARRFQVVKVEEPTEETAIIMMRGLTEPLENHHKVRILD
                     EAVEDAVRLSHRYISGRQLPDKSVSVLDTACARVAIGQAATPPAVEDAQRAIDHSAAE
                     IEVLAREEAVGTDHKERLAELTARKAAAEESLGRLKAQWDKERELIGKIRDIRSTLED
                     LAAPKKAPAAKAAGPGAKPAPATAAPAAAAVPAGPTPDPAALKAELGALNAELDKLQG
                     ESPLMQACVTSQTIAEVISGWTGIPVGKMLTDEIRTVLSLKDRLEQRVIGQSHALEAI
                     ASRIRTARANLTDPRRPIGVFLLVGPSGVGKTETALTLAETLYGGEHNLITINMSEYQ
                     EAHTVSSLKGSPPGYVGYGEGGVLTEAVRRRPYSVVLLDEVEKAHPDVMELFYQVFDK
                     GMLEDGEGREIDFKNTVILLTSNAATDTLMKLCADPETAPEPDKLVEAIKPELNKIFK
                     PALLGRMVIVPYYPISDKVMREIIKLQLGRIGSRLRENHGAAFSYDEKVIAEIVARCH
                     EVESGARNVDHILTRTVLPEMSGEFLARMAEGRTMSRVHISVGDGGGFRYEFD"
     gene            11815..14511
                     /gene="tssI"
                     /locus_tag="PLB01_07525"
     CDS             11815..14511
                     /gene="tssI"
                     /locus_tag="PLB01_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HQQ77190.1"
                     /translation="MPYTQSGRRLKVTTPLGPDVLILNGFSGHEAVSRPFLFQLECVA
                     ENVKAKQVVFDALLGQKITAEIALPGGAPRFVNGTCLRVAEGARDATFTHFRLDLVPE
                     PFLLSRKTQSRIFQQASIPSILKEVLKDFDVSYEITGTFHPRDYCVQYRESDFAFVSR
                     LMEEEGIYYFFKHSDGSHVMVVANTPGSHSDLPGKSALIYETVGGGSREEDRVQAWEK
                     SQELRSGKVMLWDHSFELPHKHLEIEYPIEAAVPAGKASHKLKLGANAKLELYDYPGG
                     YAQRFDGINKGGGEEPSEIQKIFKENLRTAEIRMQSEAAASVLVHATSNCRQVVSGHK
                     FTLERHFDGDGPWVVYEVEHAASEAADLRSGGGGFTYQNRFTCFPVALPFRPPRVTPI
                     PTVRGTQSAVVVGPPGEEIFTDKYGRIKVQFHWDREGAYDSDSSCWIRVVTPWAGHRW
                     GAIHIPRIGQEVVVDFLEGDMDQPIVIGGLYNADCMPPYELPKYKTYSTMKSRSTRDG
                     GNMDFNELRYIDTYGKEHVFLHAQRRMDVRVKKNKYECIGGTSSISVGGDQLHTIGGD
                     YNLHAKGKWYLEVDSDIDAYAKGNIKLGTAADIRVGAVRIEANAKRMVLDGGSELIFR
                     VGGNFVRIDGSGVTIQGTLTRINGGGGAPPAPVPDIDGPLDAGGCDTGEPGYLDRPYK
                     GGGGGRKHWNAYNGSGLDIGGGKTSPVFRNDDGSVSVGNSITIKPSSKDPQFTDKVLD
                     DLGAISSTPSGKARLGKIEDSGHTVNIERIDNGANAQEISSNNNDAAKAGAPAFGGGT
                     GTGKGSDSTVQYNPDFKPPTVGPDPAHPLTPPGTVPSDDILFHELGHTEHGVQGQQDA
                     TALPTQPDGRQFDNKEEKATTDEENTYRDERGLPRRFDHHNL"
     gene            14576..15175
                     /locus_tag="PLB01_07530"
     CDS             14576..15175
                     /locus_tag="PLB01_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77191.1"
                     /translation="MPEGQTSPAAEAVRDKLRVGLTVTAEAARLVVETTVSNSSAQTI
                     HVFNVLWDYAPSGSIGEPDSPAWVCFENGELRLARRQLPYPVGRKVLVTIEPCLTAIA
                     PGAVLREQMSFPVPVQEYSCYFRRQDDSPVEPVESSTARFAYGIVVGASEGAFGPAPM
                     PGALRLINATRTAGVLTVESGAVPCKVQVLRRRDAFQRF"
     gene            15198..>15449
                     /locus_tag="PLB01_07535"
     CDS             15198..>15449
                     /locus_tag="PLB01_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77192.1"
                     /translation="MPQGPAARLTDAVVHVPPVLTGGPPALTVLIGNLPAWKGIPLAA
                     APGLVAAQQASEAAIKTAENIAIAAQAAAVAAAGTPAAPA"
BASE COUNT         2844 a         5351 c         4936 g         2318 t
ORIGIN      
        1 cggacccagc gccgccgccc gctcccggcg cccccgcccg ggcccctgat cccgaggccc
       61 tggcggcggc ggtccctcgc ccggagccgc ccggctggga cctgccgctc gcctccctcc
      121 tcggaaccgg ctgagccggc tctccggccg cccggcgaga acgcgactca aatcttacgc
      181 atcccgcgcc cccgggcata agatgcgcgc cacggtccca aaccgggtga aaggaaatcg
      241 tggccatggc gctcgtcgac atgttcctga agctcgacgg agtcgaaggc gaaagcaagg
      301 atgcgtcggg caagggcggc tggatccaga tcagcggttt tcgcatcggc gccgtgagcc
      361 cccgcgacgc gttcacgaac caggccaagg gagcggtcca gatgtcgcac ctgacgatcc
      421 gcgcgaaggt cgacaagtcg acgccgaagc tcttcacgaa gatcaagacg aacgagaaga
      481 ttccgaccgc ggtcctgtcc tgccgcaagg cgggcaagga acagttcgag tacttctcga
      541 tcacgctgag cgaggccctc gtcgtgaggg ttcaggccgg cagcctcgag tccatgggca
      601 gcgacgtggt cccggagtgc gaattcgacc tcgcgtacgg gaagctcgag atgaagtcga
      661 tggagcagac gagcatgggt ccgacgtcgg gcccggtcgt gttcagcttc aacctgatgt
      721 cgaacgcgtg agcatcaccg tgacgaactc ccctgcgcgg ccgcacgagg gatccgttca
      781 tgttgaccgc cgtcacgtgt ctgtcgccga ggggttgaat tccatcgcgc gcaacctagg
      841 tttgataccg agagcgagtt gcgatcgtga catcatcaac ggagagacga gggagccatc
      901 aagaacatcc gccgagcgcg gatagaagga gcagcccatg gccgtagtcg actatttcct
      961 gaagcttgac gggatcgatg gcgagagcaa ggacgacaag tacaaggacc acatccagct
     1021 ggagagctgg tcgtggggcg agacgaacgc gagctcgggg cacgtgggaa gcggacacgg
     1081 ctccgggaaa gtcagcgccc aggacttcca tttcgtgatg aaggtcaaca aggcgacgcc
     1141 gaagctgatc ctcgcctgtg ccgaaggcca ccacatcccg aacgcgacgc tgatcgcccg
     1201 gaaggcgggc aagggccagc aggacttcct gaagtacaag ttcaccgacg tcttcgtctc
     1261 cagcttccag acggggggcc atgccggcgc ggacacgatt ccgaccgacc agatctcgct
     1321 gggctacgtc aagatggaga tcgagtacaa ggagcagcag aaggacggca gcctcggcgg
     1381 agccgtgaag accggttacg acttccaggc gaacaagaag ctcgcctgag catccaggag
     1441 ctcgggagaa gaagaccctc gcgcaagcga gggtcttctc gtttccggat caggcgacgg
     1501 ccgcgggacc gggcgggcgg aactcgacct gccgggcctc gaacatcgcc acgtcgtcct
     1561 cgtccacctg gaaggtcttc aatccggctc cacgggacag gtcctcggcg agcggaatcc
     1621 aggtcgtcat cctgccgagc cggagctggt cgtcagcgct tcggcacgtg cccgcgtaga
     1681 gagccggcac gtagacgtct ccgaccgtcc cgtccagggc ctcgaccttc gcgcgtgccc
     1741 agacgaggtc ccggagcttc gacggggggc tgatctccag gcggcggatc tgctcgaacg
     1801 gaagccacgt gtacttgtcc ttcacgatca gctcgaggac gggcccggtc acgtcgtcgc
     1861 agtcgcggaa gtcggcgaac ggccgtccgt tcagcgtccc gggcaacgcc ggacgctctt
     1921 cttccgcccg gtcgagaaga attctggcct cggcgacgtt gccctcgcgg atccggttga
     1981 tcgcctcgag gtgccggtcc acgtagggcg gcggctcgtt caggaagtgg ggatggaggc
     2041 cttcggagaa gagccgttcc cgcgagcgct cggcctcgat gttcgccagg tagacctgga
     2101 ccgcgagcgc ctcgttcggt cccgcctgcc cgaggacctc gatctgcttc cgcgcgcggt
     2161 cccagtcacc cgcgaaggag aggagctcga acaggaagat tcgcgttggg ccgtcggccg
     2221 ggtttgcccg gacttcggcg gtcagggcct ggatcgccgc ctggagttga cccgcctgat
     2281 acagttcccg aggtgtgggc atggtttctc cttagtttct ggaccggccc ttgaccggga
     2341 tccgcgccgt cgctatcctt cgacattcta actgttggcc ttcgaatggc ataagcgtct
     2401 ttctgaccgg agggaatcgt cgcgatgcct tcagcccccg tttttaacct ggacgcgctg
     2461 ctcgcgccga tcgcggggga aaaaccgtgc ggtgagaacc tccagtactc ggggctccac
     2521 gacgagatcc gcgaggcgcg ccgcgcggac gacgacgttc cgcagggcga ctgggtccat
     2581 gagctcaagg tcgcggactg ggaaaaggtg gccgagctcg ccggcgagag cctggccagc
     2641 aagaccaagg atctccagat cgtggcctgg ctttccgaag ccgccgtcaa catgcacggg
     2701 ttcccgggcc tcagggactc gctgaagctt gcgaccgggt acctcggcaa cttctgggac
     2761 gcgctctacc cggagatcga cgaggggaac gatctcgagg cgcgcgcgaa cgcaatcgcg
     2821 tggatggacc gccaggccgc cgaagccgtg cggaaggtcc cgctcacgaa gcgttccggc
     2881 gcgaactaca acttcttcca gtgggacgaa gccctccagt acgattttcc ggccgacacg
     2941 tcgaagctct caagcgaggc gctcgctcag ctcgaggaaa agcgcgcgcg cgcccaggcg
     3001 gaaggaaaga tcacgggcga ggaggtccgg aaggccaaga gcgagacacg ccgcgccttc
     3061 tacgaggagc tcttcgcgga cgtggccgac tgctggtcgg ccctgaaagc cctcgacaag
     3121 gagatggacg cgcgctttgc gcgccagact cccgggctcg gcaacctcac gaagtctctg
     3181 gatgcgatcc ggacacttct cgagaaggtc gtcaaggaga agcggatcga ggaacccgat
     3241 ccgtccgatg cgacggaagg ggccgaaggt gaagcggcgg ccggcggcgg cggcgggccg
     3301 ggcgggacca tccgggcacg ggaccaggca ctcgcccagc tcgcgcaggt cgccgactac
     3361 ttccggagga ccgagccgca cagcccggtc tcgtacctcg tccagcgcgc catcgcctgg
     3421 ggccagatgc ctctcgacgc ctggctcgtc gacgtgatca aggacaccaa cgttctcgcc
     3481 ggcctgcgtg agactctcgg tctcaaggac gagtccggcg gcacgggaac ctgagggagg
     3541 gagtcatgcc gaagaaggaa agcatccagc aaaaactcaa gaggatccgc cctccccgcg
     3601 tccagatcac gtatgacgtc gagatcggcg gggccatcga gatgaaggag ctgccgttcg
     3661 tggtcggagt cctcggcgac ttctccggtc agcccgccga gccgctcccg cggctcaagg
     3721 accggaagct gatcgagatc gaccgcgaca acttcgacca ggtccttgcc gcccaggcac
     3781 cgcgcctcgc ccttcgcgtc gacaacaagc tctccgacga cggcagcaag atgaacgtcg
     3841 agatgaagtt caacaacatg acggacttcg agcccgatcg catcgttcag caggtcgagc
     3901 ctctccggaa gctcgtcgaa gcccgccaga agcttcgcga catcctttcg aaaaccgacg
     3961 ggaacgacaa gctcgaggcc atgctgacgg atcttctgaa ggacccgaac gcccagaagc
     4021 agctcggcaa gcagctcggc gtcgtgacgg acgaggagcc gaagaaggaa gaaccccatg
     4081 agtaagaacc ccgagaagaa ggccgagtcg gtcgtccagg agcaggaggg cggtcttctc
     4141 aacaagattc tcgagagcgg acgcctcgcg ctgcgcgccg accaggtcga caacgcgaag
     4201 gacatgctgg ccgagttcgt ccagcaggct cttcaggggc aggtcgttct ctccaaggac
     4261 ctcgagcaca cgatcaattc gcgcatcgcg gcgatcgaca agctcctgtc ggcgcagctc
     4321 aacgagatca tgcaccacgc cgatttccag aagctcgagg ggagctggcg cgggctccac
     4381 catctcgtgt tcgagagcga gacctcgacg atgctcaaga tccgggtcct gaacgtgtcc
     4441 aagaaggacc tcctgaagga cctcgagcgg gccgccgagt tcgaccagtc cgcgctgttc
     4501 aagaagatct acgaggagga gtacggaaca ttcggcggcg cgccgttcgg cgccctgatc
     4561 ggggactacg agttcggcaa ccacccgcag gacatgtcgc tcctcgagaa gatctcgggc
     4621 gtcgcggccg cggccaatgc gcccttcatt tcggcggcct cgcccgagat gttcggctgg
     4681 cagtcctaca ccgagctgac cgaagtgcgc gacctcgcca agatcttcga ccggacggag
     4741 tacgcgaagt ggcgctcctt ccgcgactcg gaggactcgc gctacgtcgg cctcaccctc
     4801 ccccacaccc tcatgcgcct tccctacggg aaggatacgg tcccgacgga gagcttcgac
     4861 ttcgaggagg acgtggacgg caaggaccac aagaagtacc tctgggggaa cgccgcctac
     4921 tcctacggga cgcgcctgac cgaggcgttc gccctctaca actggaccgt cgcaatccgc
     4981 ggggtcgagg gcggcggcct cgtacagggg cttccggccc acacgttcca gaccgacgag
     5041 ggcgacgtgg ccctgaagtg cccgaccgag atcgcgatca ccgaccgccg cgagaaggag
     5101 ttctcggatc tcgggttcat ctcgctcgtc cactgcaaga acaccgacta cgcggcgttc
     5161 ttcggcggcc agtcggccca gaaagcgaag aagtacgaca ccgacttcgc gaacgcgaac
     5221 gcgcgtctct cgacgcagct cccctacatc ttcgccgtgt cccgcttcgc ccactacctc
     5281 aaggcgatca tgcgcgacaa gatcggcagt ttcgcctcgc gcgccgacgt cgagttcttc
     5341 ctcaacaagt ggatctcgaa ctacatcacg cccgacgaca cggcgtcgcc ggccgtcaag
     5401 gcgcagttcc cgcttcgcga ggcgcggatc gaggtggcgg aggtcgcggg gaagcccggc
     5461 gtctacaagg cgatcgcctt cctcaggccg cacttccagc tcgacgagct gtcggtgtcc
     5521 ctgcggctgg tggccgagct gccggctccg acgagaaagt aaggacggaa caccggaggc
     5581 cggcgcatcg ccgccggcct ccggcatccg ccccggcaag tcaccatgtc gaggatcgtg
     5641 gacgagatcc ggatcaacct gtcggtcctc gaccggctgg tggacttcga gccggaagtg
     5701 aaggccgagc ccccgacgtc gcgctcgaag gccattcgcc aggtgaaaca ggccctcaag
     5761 cgcgacctcg aatggctcct caacacccgc cgggcgttcg aggtgcccac cgagctgccg
     5821 cttctctccg actccctgct cgcgtacggt cttccggatt tctcgaacgt cagcgtgaag
     5881 aactcgggcg atcagcacaa cctcacactc gcggtcgaag ccgcgctccg gcggttcgag
     5941 ccacgactcg aggacgtcgt cgtcagcgtc gcgagcgcgt ccgtgctcga gcgcgccttc
     6001 cgcttccgga tcgaggcgcg tctgcgggtc gaccccgttc ccgagccgat ctcgttcgac
     6061 acgacgcttc agatcgggag cggcaacttc gccgtcaagg gcgactgatg cgcagcgagc
     6121 tcctggagta ctacgagcgc gagctgagct tccttcgcca gatgggagcg gagttcgccg
     6181 acaagtaccc gaaggtcgca tcccgcctcc agctcgagcc cgacaagtgc gaagacccgc
     6241 atgtcgagcg gctcctcgag gcgttcgcgt tcctcgcggc gcgtgtgcgc ctcaagatcg
     6301 acgacgagtt ccccgaggtc acggaatctc tcctcggcat cctgtacccc accttcctcg
     6361 ctcccgtacc gtcgatgtcc gtggcgcagt tcgtcctcaa ccccgagcag gtcagcctcc
     6421 agacggggca gaccatcgcg aagggctcga cgctcgcgtc cgcccccgtc gacgggatcc
     6481 cgtgccgctt ccggaccgcc tatccggtca cgatctggcc cgtcgaagtg aaggcggccc
     6541 gcttcgagca ggcgcccgcc gtcacgatcg acggccggga cgcgcggacg atgctccatc
     6601 tcgacctgcg catcctgggg ggcgccgcac tctccgagct gaaggagaag acgtccgacg
     6661 gcgaggaacg cccgctctcc aggctccgtt tctacctcca gggcgagggc aaggtcgtcc
     6721 atgcgctcta cgagctgctc ttcaacgacg cgctcgccgt cgagctgagg ccccaggccg
     6781 cggctcccga ctccgccgcg atcgtcctcg gccccgaggc gctccgcccg gtcggcttcg
     6841 gcgacgacga gacgctcctg ccgacgcccg accgcctgtt ccggggatac cgcgtcctcc
     6901 aggagtactt cgccttcccg gagaagttcc tcttcgtgga tctcgacggg ctcgaccgcc
     6961 tccagggccg cggcttcacg gacacgctcg acgttcggat cctcctcggc cgtccgttcg
     7021 ccgcggagcg gagcgtctcc gcgcagacgt tccggctcca cacgaccccg atcgtcaatc
     7081 tcttctctca gatggccgag cccatccgcg tgacgcacct cagccacgag taccgggtca
     7141 tcccgaatct ccggcggccg ggcgcgcacg aggtctggtc ggtcgacagc gtcacgtcca
     7201 cggccgcgga cctcgagacc gtgcgcgcat accagccgtt cttcgcgttc cgtcacaaca
     7261 cggagcgcac gggccatgac gcgttctggc acgcgaaccg acgcccctcg gagcgcaagg
     7321 acgacgacgg gaccgaggtg tacctcacgc tcgtcgacat gaacttcgac ccgagcgtgc
     7381 cggacaccga caccctcacc gtgcatacga cgtgctcgaa ccgcgatctc ccggcgcgcc
     7441 tcccgttcgg caatcccgac ggagacttcc agctcgaggg gcctggcgtc ttcaccgcga
     7501 tccgaagcct cgtgaagccc accgcgagcc tccggcctcc gctccggcgc ggcgcgcact
     7561 ggaggctcat ctcgcatctc gcgttgaacg tcctttctct cgtcgagcgc gagggagaga
     7621 agggcccgga ggctctgcgg gagatcctgc ggctctacga catcgccgac tcgtcggtca
     7681 cccggcagca gatcgacggg atttcgaggc tctccgcacg ccgggtcgtc cggccgctcc
     7741 gcgacattca cccggggttc gtgcggggac tcgaggtttc gatcgaattc gacgagcaga
     7801 acttcgtcgg ctcgggcatc tacctcttcg cgtccgtgct cgagcgattc ctctcgctct
     7861 acgcgtccgt gaactccttc agccagctcg tggcgtcgac gaagcagcga gagggcgtca
     7921 tgcgccgctg gcctccgcgc acgggcgacc agatcgtcct ttgacgaaat gaccgaacgc
     7981 ctccggacgc gccccgagca ctttgggttc ttccaggccg tccggctcct gtcgaggctt
     8041 tcgccgtcga gggctccggt cggacaggac gggcccccgg cgcgcgagat cgcgcgcttc
     8101 cggacgcgcg ccaccctcga gttcccgccg agcgagatct tcgagctccg cccggacgcg
     8161 acggatccgg accgtccgcc cgaagtcacc gtcgcgatcg gggggacgac gggtccgctc
     8221 ggggtcctgc ccacaccgta caccgaactc ctcatcgaac gggtccggta caaggactac
     8281 gcgctctggg agtttctcga cctcttcaac caccgcttcc tctccctgtt ccatcgcgcc
     8341 tggcagaagt accgcttccc cgtcgtgtac gaaagcggcg gcgacgatcc cttcgtcgag
     8401 ggcctgttcg cgctcatcgg cctcggcacg ggctctctcc gcgcccgcca gagcgtcccc
     8461 gacgacgcgc tgctccacta cgcgggcctc gtggcgcagc ggccgcattc gtcgagcgcg
     8521 ctcgtgggca ttctccgcga ctacttcggc gtccccgtcg cgaaccggca gttcgtgggc
     8581 cagtggctcg acctcgagcc ggaggacagg tcccggctcg ggtcccggaa cagccggctc
     8641 ggcgtcgatc tcgtctgcgg agaccacgtc tggaacaacc agtcccgatt ccggatccgg
     8701 ctcggtccgc tgagcctcgc ctccttccag aagtttctgc cgcgcggaaa cgcgtggagg
     8761 ccgctgaacg acctcgtgag gttcctcgtc ggtgtcgaat acgacttcga cgtgcaaccc
     8821 gtcctgaaga aggaagagat ccccgagtgt cacctgaggt ccggcgccgc ggtccctccc
     8881 atgctcgggt ggacgacgtg gctcaagagc gccccggccc ccgccgacgc ctcggacctg
     8941 atcctcgagt ccatgaatta ggatacagac tcattccaga ttctcgagag gaaggccacc
     9001 atgggcgcaa acctgaaatc cctcatcggc cgactgaacg acacctgccg aaacgcgctc
     9061 gagggagccg ccgggctctg cctgtcccgg acgaactacg acgtcgacgt cgagcacctg
     9121 ctcgtcaagc tgggcgagac gccggacacc gacttccacc ggatcctgcg ccggttcgag
     9181 gttgacgagt cccggctcgc gaaagacctc gtccgatcgc tcgaccgtct caagacgggc
     9241 aacacccgca ccccgtcgct ctccccccgt ctcccgaaac tcgtcgagga agcctggctc
     9301 gtcggctcga tcgacttcgg cgcctcccgg gttcgctcgg gacacatcct cctcgccctt
     9361 ctcgagaaag acgagtttgc ccgtctcgca cgcgagatct ccaaggagct cgcgttgatc
     9421 tccgtcgagg cgctccggaa ggaattcgac aagatcgtct cgggctcggt cgaggaccgc
     9481 gacgcagccc tcctcggcga ggcgccggcc ggcgacgcgg cggggccgtc gggcgtcccc
     9541 ggacgcacga aggcgctgga tcagtacacg gtagacctga ccgcgcgcgc gcggtccggc
     9601 cacatcgacc cgatcctcgg acgggacttc gagatccgcc aggtgatcga catcctcacg
     9661 cgccgtcggc agaacaaccc gatcctcacc ggagaggccg gcgtcgggaa gacggccgtc
     9721 gtcgagggat tcgcgctccg catcgcccag ggcgacgttc cgggccctct gaagaacgtc
     9781 gccgtgagga ccctcgacct cggactcctg caggccggcg ccgggatcaa gggcgagttc
     9841 gagaaccgcc tcaagacggt catcgaggaa gtgaaatcct cgccgcagcc gatcatcgtc
     9901 ttcatcgacg aggcgcacac gatgatcggg gccggcggcc aggccgggca gaacgatgcc
     9961 gcgaacctcc tgaaaccggc tcttgcccgc ggagagctgc gtacgatcgc cgcgacgacc
    10021 tgggccgaat acaagaagta cttcgagaag gacgctgcgc tcgcgcggcg cttccaggtc
    10081 gtgaaggtcg aggagccgac cgaggaaacc gccatcatca tgatgcgcgg cctcaccgag
    10141 cctctcgaga accaccacaa ggtgcgcatc ctcgacgagg ccgtcgagga cgccgtccga
    10201 ctctctcacc gctacatctc gggccgccag ctcccggaca agtcggtcag cgtgctcgac
    10261 acggcatgcg cacgtgtcgc gatcggccag gccgcgacgc ccccggcggt cgaggacgcg
    10321 cagcgggcga tcgaccacag cgcggcagaa atcgaggtcc tcgcgcgcga ggaggccgtc
    10381 ggcaccgacc acaaggagcg cctggccgaa ctcaccgcgc gaaaggcggc cgccgaagag
    10441 agcctcggcc ggctgaaggc gcagtgggac aaggagcgcg agttgatcgg aaagatccgc
    10501 gacatccggt cgacgctcga ggacctcgcg gcccccaaga aggcgcccgc agccaaggct
    10561 gcgggacccg gcgccaagcc cgcccctgca accgccgccc cggccgcggc cgcggtcccc
    10621 gcgggcccga cacccgaccc ggcggccctc aaggccgagc tcggtgcgct caatgcggag
    10681 ctcgacaagc tgcagggcga atcgccgctc atgcaggcct gcgtcacgtc ccagacgatc
    10741 gcggaggtca tctccggctg gacggggatc ccggtcggaa agatgctgac ggacgagatt
    10801 cgaaccgtcc tttccctgaa ggaccggctc gagcagcgcg tcatcgggca gtcccacgcc
    10861 ctcgaggcga tcgcgagccg gatccgcacc gcgcgggcga acctgacgga cccacgccgg
    10921 ccgatcggcg tcttcctcct cgtggggccg agcggagtcg gcaagaccga aaccgccctc
    10981 acgctcgccg agacgcttta cggcggcgag cacaacctca tcacgatcaa catgagcgag
    11041 taccaggagg cgcacaccgt ctcgtcgctg aagggctcgc ctccggggta cgtggggtac
    11101 ggcgagggcg gtgtcctgac cgaggccgtg cggcggcggc cctactcggt cgtcctcctc
    11161 gacgaggtcg agaaagcgca cccggacgtc atggagctct tctaccaggt cttcgacaag
    11221 ggaatgctcg aggacggcga aggccgcgaa atcgacttca agaacaccgt catcctgctc
    11281 acgtcgaacg ccgccacgga caccctcatg aagctctgcg ccgatcccga gacggcgccc
    11341 gaaccggaca agctcgtcga ggcgatcaag cccgagctga acaagatctt caaacccgcg
    11401 ctgctcggtc gcatggtcat cgtcccgtac tacccgatct cggacaaggt catgcgagag
    11461 atcatcaagc tgcagctcgg gcggatcggc agccggctga gggagaacca cggcgccgca
    11521 ttcagctacg acgaaaaggt catcgccgag atcgtcgcgc ggtgccacga agtcgagagc
    11581 ggtgcgcgca acgtggacca catcctgacg cggaccgtcc tcccggagat gtcgggcgag
    11641 ttcctcgccc ggatggccga aggccggacg atgtcgcgcg ttcacatcag cgtcggtgac
    11701 ggcgggggct tccgctacga gttcgactga tggggccccc gggcccgcgc cccgaccgcg
    11761 agacttccga agcgaattcc tgcgaccggt ccgggcggga ttgaggagcg ccgagtgccc
    11821 tacacccaat ccggccgacg gctcaaggtc acgactccgc tcggccccga cgtcctgatc
    11881 ctgaacgggt tctcgggaca cgaggccgtc tcgaggccct tcctgttcca gctcgagtgc
    11941 gtcgccgaga acgtcaaggc gaagcaagtc gtgttcgacg ctctcctcgg ccagaagatc
    12001 accgccgaga tcgccctccc cggaggcgcc ccgcgcttcg tcaacggaac ctgcctgagg
    12061 gtcgcggagg gcgcacggga cgccacgttc acgcacttca ggctcgacct cgtcccggag
    12121 ccgttcctcc tgtcacgcaa gacgcagagc cggatcttcc agcaggcctc gattcccagc
    12181 atcctcaagg aagtcctgaa ggacttcgac gtcagctacg agatcaccgg aacgttccat
    12241 ccgcgcgact actgcgtgca gtaccgcgag tccgacttcg cgttcgtgag ccgcctgatg
    12301 gaggaagaag gcatctacta cttcttcaag cacagcgacg gcagccacgt catggtcgtc
    12361 gcgaacacgc cgggaagcca ctcggatctc ccggggaaga gcgcgctcat ctacgagacc
    12421 gtcgggggcg ggtcccgcga ggaggaccgc gtccaggcct gggaaaagtc acaggagctt
    12481 cgatcgggca aggtgatgct ctgggaccac agcttcgagc tgccccacaa gcacctcgaa
    12541 atcgagtacc cgatcgaagc cgcggtcccc gccgggaaag cgagccacaa gctgaagctc
    12601 ggcgcgaatg cgaagctcga gctctacgac tacccgggcg gctacgcgca acgcttcgac
    12661 ggaatcaaca agggcggcgg cgaagagccg tccgaaatcc agaagatctt caaggagaac
    12721 ctccggaccg ccgagatccg gatgcagtcg gaggcggccg ccagcgtcct cgtccatgcc
    12781 acgagcaact gccgccaggt cgtctccgga cacaagttca ccctcgaacg ccatttcgac
    12841 ggcgacggcc cgtgggtcgt ctacgaggtc gagcacgcgg cgagcgaggc ggccgacctg
    12901 cgctccggcg gaggcggctt cacctatcag aaccgcttca cctgcttccc ggtcgccctg
    12961 cccttccggc cgccccgcgt cactcccatt cccaccgtcc gggggacgca gtccgccgtc
    13021 gtcgtgggcc cgcccggcga agagatcttc acggacaagt acggacgcat caaggtgcag
    13081 ttccactggg accgcgaggg cgcgtacgac tccgacagct cgtgctggat ccgggtcgtg
    13141 accccgtggg ccggccaccg ctggggcgcg atccacattc cccgcatcgg ccaggaggtc
    13201 gtcgtcgact tcctcgaagg cgacatggac cagccgatcg tcatcggcgg gctctacaac
    13261 gcggactgca tgcctccata cgaactgccg aagtacaaga cgtactccac catgaagtcg
    13321 cggtcgacgc gagacggcgg caacatggac ttcaacgaac tgcggtacat cgacacctac
    13381 ggcaaggaac acgtgttcct ccacgcgcag cggcggatgg acgttcgcgt gaagaagaac
    13441 aagtacgaat gcatcggggg cacgagttcc atcagtgtcg gcggcgacca gctccacacg
    13501 atcggcggcg actacaacct gcatgcgaag ggcaagtggt acctcgaggt cgactccgac
    13561 atcgacgcct atgccaaggg caacatcaag ctcgggaccg cggccgacat ccgggtcggc
    13621 gccgtccgta tcgaggccaa cgccaaacgc atggtgctgg acggcggaag cgagctgatc
    13681 ttccgggtcg gcggcaactt cgtgcgcatc gacggctcgg gcgtcacgat ccaggggacg
    13741 ctgacgcgca tcaacggcgg cggaggagcg ccgcccgcgc cggtcccgga catcgacggg
    13801 cctctggacg caggtggctg cgacaccggc gaaccgggtt atctcgatcg accctacaag
    13861 ggaggaggag gaggccggaa acactggaac gcctacaacg gttccggtct ggacatcgga
    13921 ggcggcaaga cgtccccggt gttccgcaac gacgacggct cggtcagcgt cggcaattcc
    13981 atcacgatca aaccgtcatc caaggatccg caattcaccg acaaggtact ggatgacctc
    14041 ggcgccatct cctcgacgcc ttccggcaag gcccgactgg gaaagatcga ggactcgggc
    14101 cacaccgtga acatcgagcg gatcgacaac ggagcaaacg cgcaggagat ctcctccaac
    14161 aacaacgacg ccgccaaggc cggggcaccg gccttcggcg ggggaaccgg caccggaaag
    14221 ggttcggatt cgacggtgca gtacaacccg gacttcaagc ctccgacggt cgggcccgac
    14281 ccggcccacc cgctgactcc gcccgggacg gtgccttccg acgacatcct gttccacgag
    14341 ctggggcaca ccgaacacgg cgttcaggga caacaggacg ccacggcgtt gccgactcag
    14401 cccgacggcc gccagttcga caacaaggaa gaaaaagcga cgacggatga agagaacacg
    14461 tatcgtgacg agcgcggcct tccccgacga ttcgaccatc acaacctctg aggaagcgac
    14521 gtcacaagga gcgggtggaa cgacatgatg ggtcacgacc gcgcgggagt cacgaatgcc
    14581 cgaaggccaa actagccccg ccgcggaggc cgtccgcgac aagctgcgcg tcggcctgac
    14641 ggtgacggcg gaggcggcgc ggctcgtcgt cgagaccacc gtctccaatt cgagcgcgca
    14701 gaccatccac gtcttcaacg tcctgtggga ctacgcgccc tcgggcagca tcggcgaacc
    14761 cgacagcccc gcctgggtct gcttcgagaa cggggagctt cggctcgcgc gccggcagct
    14821 tccctacccg gtcgggagga aggtcctcgt cacgatcgag ccctgcctga ccgcgatcgc
    14881 gcccggcgcg gtgctgcgcg agcagatgtc cttccccgtt ccggttcagg agtactcgtg
    14941 ctacttccgg cggcaggatg attcgccggt ggagcccgtc gaatcctcga ccgcgcgctt
    15001 cgcgtacggc atcgtcgtgg gcgcttcgga gggggcgttc ggacccgctc cgatgccggg
    15061 ggctcttcgc ctcatcaacg cgacccggac ggccggcgtc ctgacggtcg aatccggtgc
    15121 cgtgccgtgc aaggtacagg tcctgcgccg acgagacgcc tttcaacgtt tctgacccat
    15181 caaggacacg aatcgacatg cctcaaggtc ccgcagcccg cctcacagac gccgtcgtcc
    15241 acgtcccgcc ggtcctcacc gggggtcctc cggccctgac cgtcctgatc gggaacctgc
    15301 cggcgtggaa agggatcccg ctcgccgcgg cgccggggct ggtggcggcg caacaggcct
    15361 ccgaggccgc catcaagaca gcggagaaca tcgccatcgc cgcccaggcc gccgccgtcg
    15421 cggcagccgg gactccggcg gccccggcc
//
LOCUS       DAOMFY010000067        15399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1182_length_15399_cov_7.834724, whole genome shotgun sequence.
ACCESSION   DAOMFY010000067 DAOMFY010000000
VERSION     DAOMFY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1182_length_15399_cov_7.834724"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PLB01_07540"
     CDS             <1..954
                     /locus_tag="PLB01_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ77193.1"
                     /translation="LAEANGPARAVLGLGDADVGRPASEALADVPVLASAAERARAGE
                     PTLSTEFALGDDTGGRLLEVIAVPILDAGASVLGALLFVEDRTETRRLEREVSSRREL
                     AALGEMSAGIAHEFRNATGTILGWARLAARTDDAAARGRHLEAIQAEAEHVARVTGDF
                     LFFAKPERLETAPCDLGALAREIAAEQKVITPAVEVSVAGTFAPAQADAALVKRALVN
                     LVKNACEAVLDRPGGGRVVLSGDSGAGTVRVAVEDDGPGVPEESAARLFVPFYSTKEA
                     GTGLGLALVAKIAALHRGGISVERSAALGGARFVLSLPSAR"
     gene            complement(959..2086)
                     /locus_tag="PLB01_07545"
     CDS             complement(959..2086)
                     /locus_tag="PLB01_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQQ77194.1"
                     /translation="MNSSVHAGVNRVDKLLRIMVDKKASDLHLAVGSPPMVRVDGDLE
                     RLKWRTLTEEEFESLIVPIAPEKPLREWRATGDTDFAYAMGEIARFRVNVFRQEHGSG
                     AVLRMIPPRVLAIEELGLPALVANVARITRGLVLVTGPTGSGKSTTLAALLDWINRTR
                     SAHIITIEDPVEFIHRPAKSVISHRELGFDTPTFSGALKAALREDPDVVLVGELRDLE
                     TMSMALQAAETGLLVFGTLHTNSAAKAVDRLIDSFPAEEQEQVRTVLAEVLKGVVAQV
                     LLRKKGGGRAAAFEVLRGSSALSNNIREGKTAMINTLIQTGRNAGMIGMDQSLADLVV
                     AGTVESEEAFEKALDKEQFRGLLDKRTKAAAKPVPAAPPAG"
     gene            complement(2083..4605)
                     /locus_tag="PLB01_07550"
     CDS             complement(2083..4605)
                     /locus_tag="PLB01_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQQ77195.1"
                     /translation="MLEKLRGLRSEPAFIAALREHKWSSEDQKKDLLEKFQALPKPDV
                     EWVAWTAVDSVPEIRTAGLAILKRRHDRAGLEALVPHLKTRSEAVRRAVMRFLKDLAG
                     PQLGPFLQEVASRGDDFARLAALELARELPAQVAFDIARRVLTASNPAVRARALKAVS
                     EMNFEGSADVAASLAVPLLQDENEEIRYSALAVLEKNPNESYFPDVLQMARTGGGRVM
                     EASFGALKKLLATAKQDHTPEIVPLLADGNALVRAGTVSLLLPYAPDVLARRILEHFR
                     GSYVWVRDRAVETARDGLPGFVNALLVLTRDPDPAFAKAASEMTLALTDAKALPVWIA
                     LLDDKDIWVKTRALETLGRLGKGDDAVLTRVLAALRDPELAIPAVSALGDLGDARAAQ
                     PLLDAFKAAVTQPDVQMEILDAMAKLASDEKMQTQIAGICARVAQMPALDGKVREKAR
                     RTVGILKGTEARDALPEVEAEVQTVELAGNDAARLVEYLADTVKRGASDLHLATGFVP
                     HRRQQGILEPVDAPVMTRARASQLIREVLPEDDWKRLEKERQIDVCLKLPGLGRFRAN
                     FFSQRGGFDACFRVVPKTIPTIEEIGLPGPTTADILKLTQGLVLVTGPSGCGKSTTLA
                     ALIDRINETRPCHIITVEDPVEFVHPNKEALVNQRQVPHHTASFARALKAALREDPDV
                     ILVGEMRDLETISLAITASETGHLVLGTLHTTNAPSTVDRVISSFPPGQQGQIRVMLA
                     DSLKAVVSQALLPRRGFAGRVAAFEILRTTPAVAALIREGKTHQIPSALQTGQLSGMC
                     TMDQSLMKLCEEGKVDPELAMEKAVKKEPFEKLLAEERQALE"
     gene            4828..5592
                     /locus_tag="PLB01_07555"
     CDS             4828..5592
                     /locus_tag="PLB01_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQQ77196.1"
                     /translation="MTMSLPEPGCRTVNGPSASGGRRGFSLVEMLVVIFILAIVFIIG
                     GSGVARAWKRQKLQSASMDIKVLMQRGLPEMQRRNMVTFVQVGPLVNNASVRYLPIRL
                     IGDANGNGAMDAAANPPTVASPDLLLDEYDIVVTGKSGVKGSTGVSQDFCLSTLDITQ
                     VQSALWSNNSTDWTVGRAIMCDFQGRAIDVTTGRQLAGEATLVLSHVDVVNRSYMPPT
                     RFILSINPVWSVRVRKQTTAGDPTDSGAVWVDQQGG"
     gene            5594..6145
                     /locus_tag="PLB01_07560"
     CDS             5594..6145
                     /locus_tag="PLB01_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77197.1"
                     /translation="MRRTTRGSSFIEVLLAMTIMALVLVGILQMFSVSLVINKGSAAR
                     TQMLFKCQQVVENVRLFYHLTRLGSTTPPGITESGHVSGTAMTTPGALPDVLGDVSKS
                     FLPYNQAESAASWAYWGPAGANVMEQENGPYKISYTVARDNVQNLWVITVSATPTDVA
                     TATRYFGVDLKSGKRVDYVAQFP"
     gene            6126..7472
                     /locus_tag="PLB01_07565"
     CDS             6126..7472
                     /locus_tag="PLB01_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQQ77198.1"
                     /translation="MWRSSRKALGLRRGLTLNRRGFTLIEAMVSLLLLVIVLTVAMTM
                     LFQMRAFAERQQFFMLPRQAARRATDYLSYYVASASDANDVQNNPNALIMYYNLNGNV
                     LQASYDNLTGAEPGNSVTTPNVTTQFGDIGTDVITLVAPINPAKFRVFPPFPSFTGGA
                     KNIYVNFRVGCGGPAGTDDVTNMAQFKAATGFDGTNSALLMFEDTNGEWSYFQIPAAG
                     YIASDCSDVTTYRNVHVRAAIQSAALPAPPNGGVAVFNDPVSLVTGLQFISFRVRTDP
                     VDNLPKLQQKIGLFDPNTDNPGAAFVNVMENVEDLQIAYVYANGDIWNNTAGQTITQA
                     NAGARCNVAACDNRVPTQAGPGAVTMEALDVTGVIGLRFSITARSPRLSIGARQLTNV
                     NVTDVLTTTTSQHFRPASENHPVTMDPLAPLRPLYDLFDHYRATSTMLLRNRALGS"
     gene            7475..8119
                     /locus_tag="PLB01_07570"
     CDS             7475..8119
                     /locus_tag="PLB01_07570"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HQQ77199.1"
                     /translation="MKERGSALVSVILVLLVLTIVGVGIAYFTSMEDRLSGNTRIAKA
                     GFYAADAGLRRAEVVLSGLSDLSGTCAPVSLTNLLQTGSTPTLTVPGGGYTAIPLDLT
                     GISTNACLTPLGIAQQKFYNVPVPVVAGTPVVDRATYSLYVRNNVDDASGDATVDNDG
                     LINVVSVGTVTLTAGTTLTRMLEEQLLLTTGGAFGSSQKNLNAGGTGTAQKKGR"
     gene            complement(8037..9095)
                     /locus_tag="PLB01_07575"
     CDS             complement(8037..9095)
                     /locus_tag="PLB01_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77200.1"
                     /translation="MNVEVNVGVGVLVGVGVNVKVGVVVKVYVYVAVGVGVPVGVGVG
                     VFVRVEVRVDVGVFVRVGVLVGVGVFVGVRVLVDVLVGVGVFVDVGVLDGVGVFVGVD
                     DGVIVPVHVGVTVDVGVFVGVMEGVDVGVDVDVGVHVGVAVDVGVFVGVGVDVAVGVE
                     VGVDVAVGVAVGVGVDVDVAVAVGVDVGVDVAVSVGVNVYVGVAVDVNVYVAVEVGVF
                     VAVGVAVGVGVAPGLLSRQVTVQTRRPTESSPSVRRFPYTSSFMEPLMSWRMRRFARR
                     SCAVIWRRGSNPSVAAAGAEPAPPNAMTARSRKAQARRRRSGMRNGARRISGPSSGRC
                     RSRPRSGSSGCSRRRRPS"
     gene            complement(9096..9521)
                     /locus_tag="PLB01_07580"
     CDS             complement(9096..9521)
                     /locus_tag="PLB01_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77201.1"
                     /translation="MAVGVMDGVHVGVDVGVFVNVGDGVGVFVGVLVPVGVFVGVGVF
                     VGVTVGVSVDVGVTVAVNVTVNVFVGVGVGVFVDVGVDVNVDVNVSVGVNVGVIVEVG
                     VFVGVGVFVGVFVPVVVGVFVGVIVDVGVFVGVGVVVGV"
     gene            complement(9534..9683)
                     /locus_tag="PLB01_07585"
     CDS             complement(9534..9683)
                     /locus_tag="PLB01_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77202.1"
                     /translation="MRSSNRSPGLTSGSRGRSRNDFVFVNVGVIVGVGVVVGVSVAVA
                     VAVDV"
     gene            9700..13347
                     /locus_tag="PLB01_07590"
     CDS             9700..13347
                     /locus_tag="PLB01_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77203.1"
                     /translation="MQTPAPKWGLPQPAAVVAMAGGGAARFGLAALLAPATPTPTPFP
                     SGQAGCHRWKYALHWDRPSRLATLKNALGNSVTIHKNYKAPGAGTQDDWPDPITHSVD
                     WTGAPNAGAWDWKNPPAAAATSFPYDHDCDDFVSGGPACGAPKPGDPDGEHPGIWYTI
                     GPADGAGEIVDWSWTLNYGYGKAPGNDIIYGLDPGPPLPVYDLTAEAVTGNSIPGITG
                     TPLAFPAGWTTRGGVPIAAAANQPAWQTLLPSDVVGTTRNQVNWGLMTFSDVSVDPLQ
                     SKWCTDSLNYDVKVPIVSNDTGDVTAIEEYLRLKYYSSGSHPGLSAAGGTPTKHAIEL
                     AGASLRTSWDTDPKQRCARPWGVILCTDGLSNTCNTGTTPDAEWVAAAGNTPCENDIP
                     GTDFANYPPGAAEAVYNSIMTAGGGSAIIRPRTYAIGIAPEVGRCELNRVAYRGRTDA
                     SAPKKDAGFLLYDPTEIPPPPKGDIDLPHVDPLTNDQSGPGAPPDPNHFRFDQSPAKT
                     GDYAFFALDTKSIVNAFLTIVASTATGDYSTSAPVSGAAVAAGSIVLLPSTEYPSWRG
                     HLRALDTTKVPASVGYEKWDAGDVLLNPTQPWHPTVATRSIYTWDPTTGDLIPVDTTP
                     ANVAKMAALAGDPAFTENVVDFIRGNDGTLTNTKRAWMLGPAINSTPAVVGPPYLYKQ
                     TGNVADHKPFEATYVSRRALSWVGADDGMLHAFDFDDGTEILALLPPNLLANQIALYK
                     NVPSGESDTGQKLELSQHIWGIANSFRFADVWFGTGYKTVGILTEGPAGDMVAAIDVT
                     HPYPGRAAFGPNPAIPADPNYDAAKPVEILWTKNASDYAGLHGSWSLSAIAADSFSTS
                     RMFFGAGINPASLYNAQADATLFIVDPTDGTLNQTKTIALSGTPSPLVGQQTFADSVF
                     FSTKVSGYLPDNVANLEVQADTNGRAWFTWGDFAGASTTTKVGIDLNYAAVPVVSGSQ
                     TPVPQPIYYSPSATGQGTTGCQIYALASGSLYETSPAVSGWNVNRSGAPLAPFPNTLP
                     SFTPYLYIGVNPKKITSGTFASVPLGKDPLDVFMIKQRIGGEVPEAIDLPPGDPALTG
                     TPPLSKLGLHTQVTSSPIMIVDQTGKSEEQALFLLYDPDNGCNGISYVAILTFKIPAN
                     CGQPAITQIQVYGAGPGAASGFTLTSNKLFASKSGVGRGQSAGLQEVNVPVNTLAGAP
                     SFQPVWWRDVK"
     gene            13380..14606
                     /locus_tag="PLB01_07595"
     CDS             13380..14606
                     /locus_tag="PLB01_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQQ77204.1"
                     /translation="MDAGPARRRSAGFSLAELLVTLAVLGLAAWLVLRLVDRRTRVAA
                     PEGAGSRTASVGEASLRAIVNDVRAAGTGGLSAADAFRPVEDNTTSAGRYLYRTAAGG
                     TVVVRNGTDQLGLRGVIRSPLVALAPRDAVTDALLSDRVRARPDAAAVHLPAGKAAGA
                     VRARLAEAAPPRKAFFLLRDAAGRWAVGRVVSAAEGLPESPLDVVLDFTDRDARALNH
                     GGDPDAASRLGDVSAGGVFDDLVWFVAQGEEGKPPDFVMTNDPASLAFPHPYLAAATG
                     VGADRWDVRRAGEDVEDLQVAWGFAGPSGSIAWRGDAADSPAPLAGELADAAGRARIK
                     ALKIALSVKSAHRILRSAGAPAPEFTRLFNAAPFGKIPGAAPVGWDAVPERRVSFDRE
                     TREAVVAPPALLEAGP"
     gene            14658..>15399
                     /locus_tag="PLB01_07600"
     CDS             14658..>15399
                     /locus_tag="PLB01_07600"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HQQ77205.1"
                     /translation="MRRLLIAMAVFAVGAGAAAADEIWLKDGRSITTKKPVVTKGSNA
                     LITTADGVLVSVPLSEIDQAKTAEAKARAAAPAPAPTPNLMKPMTPAEAARQKSARKA
                     VVTLTDDSVAHGFGDEAGERKAADGEGKVEVANTTATRGKDGYAFTGTVINSGAGPVT
                     GVSVTIESVGRDGKTFSSTFASVAKDALGPGEKSTFTASAPLEGEAASFRYMPRWQVP
                     IPEKTEDEKAKAQPTVAAQAEQVTDDVRR"
BASE COUNT         2399 a         5655 c         5230 g         2115 t
ORIGIN      
        1 ctcgccgaag cgaacggccc ggcccgcgcg gtgctcggcc tcggagacgc cgacgtgggg
       61 cgcccggcct ccgaggcgct cgcggacgtc ccggttctcg cgtcggccgc ggagcgggcc
      121 cgggcgggag agccgaccct ttcgacggag ttcgcgctcg gcgacgacac cggcgggcgc
      181 ctcctcgagg tgatcgccgt cccgattctc gacgccggcg caagcgtcct cggggcgctc
      241 ctcttcgtcg aggaccggac cgagacccgc aggctcgagc gcgaggtgtc gtcacggcgc
      301 gagctcgcgg cgctcggcga gatgtcggcg ggcatcgcgc acgagttccg caacgccacg
      361 ggcacgatcc tgggctgggc gcgcctcgcg gcgcgcacgg acgacgccgc ggcccgaggg
      421 cggcacctcg aggcgatcca ggccgaggcc gagcacgtcg cgcgcgtgac gggcgacttc
      481 ctgttcttcg cgaagcccga gcgtctcgag acggcgccct gcgacctcgg cgcgctcgcc
      541 cgggagatcg ccgccgagca gaaggtgatc acgccggccg tggaggtctc cgtcgcgggg
      601 acgttcgcgc cggcgcaggc cgacgccgcc ctcgtcaagc gcgccctcgt gaacctcgtc
      661 aagaacgcat gcgaagcggt gctcgaccgg cccggcggcg gccgcgtcgt cctgtcgggg
      721 gactcgggag cggggaccgt ccgcgtcgcc gtcgaggacg acgggcccgg agtgcccgag
      781 gagtcggccg ccaggctctt cgtgccgttc tactccacga aggaggcggg gacgggactc
      841 gggctcgcgc tcgtcgcgaa gatcgcggcg ctccaccgcg gcggcatctc ggtcgagagg
      901 tccgcggccc tcggcggcgc gcgcttcgtg ctctcgctgc cctcggcgcg ctgaggcgtc
      961 agcccgcggg cggcgccgcg ggcaccggct tggcggcggc cttcgtgcgc ttgtcgagga
     1021 ggccccggaa ctgttccttg tcgagcgcct tctcgaaggc ttcctccgac tccacggtcc
     1081 cggcgacgac gaggtccgcg aggctctggt ccatcccgat catccccgcg ttgcggccgg
     1141 tctggatgag cgtgttgatc atcgcggtct tgccctcgcg gatgttgttc gagagcgcgg
     1201 acgagccgcg gagcacctcg aacgcggcgg cgcgcccccc gcccttcttc ctgaggagga
     1261 cctgcgcgac gacgcccttc aggacctcgg cgaggaccgt gcgcacctgc tcctgctcct
     1321 cggcggggaa gctgtcgatg aggcggtcca ccgccttcgc ggcggagttc gtgtggagcg
     1381 tcccgaagac gaggagtccg gtctcggcgg cctgcagcgc catcgacatc gtctcgaggt
     1441 cccgcagctc gccgacgagg acgacgtcgg ggtcctcgcg cagcgccgcc ttgagggcgc
     1501 ccgagaacgt cggcgtgtcg aatcccagct cgcggtgaga gatgacggac ttcgcgggcc
     1561 ggtggatgaa ctcgacgggg tcctcgatcg tgatgatgtg cgccgaacgc gtcctgttga
     1621 tccagtcgag gagcgccgcg agcgtcgtgg acttgcccga gcccgtcgga cccgtcacga
     1681 ggacgaggcc gcgcgtgatg cgcgcgacgt tcgcgacgag cgccgggagt ccgagctctt
     1741 cgatcgcgag gacgcgcggc ggaatcatgc gaaggacggc ccccgagccg tgctcctgcc
     1801 ggaagacgtt cacgcggaac cgcgcgatct cgcccatggc gtacgcgaag tcggtgtcgc
     1861 cggtcgcacg ccattcgcgc aggggcttct cgggggcgat cggcacgatc aggctctcga
     1921 actcctcctc ggtgagcgtg cgccacttga ggcgctcgag gtcgccgtcc acgcgcacca
     1981 tgggcggcga gcccacggcg aggtggaggt ccgacgcctt cttgtcgacc atgatccgga
     2041 ggagcttgtc gacgcggttg acgcccgcgt ggaccgagga gttcattcga gcgcctgcct
     2101 ttcctcggcg aggagcttct cgaacggctc tttcttgacg gccttctcca tcgcgagctc
     2161 cggatccacc ttgccctcct cgcacagctt catgagggac tggtccatcg tgcacatgcc
     2221 cgagagctgg cccgtctgga gcgccgacgg gatctggtgc gtcttgccct cgcggatgag
     2281 cgcggccacg gcgggcgtcg tgcgcaggat ctcgaacgcg gccacgcgcc cggcgaatcc
     2341 gcgccgcggc aggagcgcct gcgagacgac ggccttcagg gagtccgcga gcatgacgcg
     2401 gatctggccc tgctgcccgg gggggaacga gctgatgacg cggtcgacgg tggacggcgc
     2461 gttcgtcgtg tggagggtcc cgaggacgag gtggcccgtc tcggaggccg tgatcgcgag
     2521 cgagatcgtc tcgaggtcgc gcatctcgcc cacgaggatg acgtcggggt cctcgcgcag
     2581 ggccgccttg agagcgcgcg cgaaggaggc cgtgtggtgc ggcacctgcc gctggttcac
     2641 gagagcctcc ttgttcgggt gcacgaactc gacggggtcc tcgaccgtga tgatgtggca
     2701 gggccgcgtc tcgttgatcc ggtcgatgag ggccgcgagc gtcgtggact ttccgcagcc
     2761 cgacggcccc gtcacgagca cgagcccctg ggtgagcttc agaatgtccg cggtcgtcgg
     2821 ccccggaagg ccgatctcct cgatcgtcgg gatcgtcttc gggacgacgc ggaagcacgc
     2881 gtcgaacccg ccgcgctgcg agaagaagtt cgcgcggaac cggccgaggc cggggagctt
     2941 caggcagacg tcgatctgcc gctccttctc gaggcgcttc cagtcgtcct ccggcaggac
     3001 ctcgcggatg agctgcgagg cgcgggcgcg cgtcatgacc ggggcgtcga cgggctcgag
     3061 gatgccctgc tggcggcggt gcgggacgaa gcccgtcgcg aggtggaggt cggaggcgcc
     3121 gcgcttgacg gtgtccgcga ggtactcgac gaggcgcgcc gcgtcgttcc cggcgagctc
     3181 gaccgtctgg acctcggcct cgacctcggg cagcgcgtcc cgcgcctcgg tccccttgag
     3241 gatgccgacc gtccggcgcg ccttctcgcg caccttcccg tcgagggccg gcatctgggc
     3301 cacgcgcgcg cagatccccg cgatctgggt ctgcatcttc tcgtccgacg cgagcttggc
     3361 catcgcgtcg aggatctcca tctggacgtc gggctgcgtg acggcggcct tgaacgcgtc
     3421 gaggaggggc tgcgcggcgc gcgcgtcgcc gaggtcgccg agcgcggaga cggccgggat
     3481 cgcgagttcc gggtcgcgca gggccgccag gacgcgcgtg aggacggcgt cgtcgccctt
     3541 gccgaggcgg ccgagcgtct cgagggcccg ggtcttgacc cagatgtcct tgtcgtcgag
     3601 aagggcgatc cagaccggca gcgccttggc gtcggtgagg gcgagcgtca tctcggacgc
     3661 cgccttcgcg aaggcggggt ccgggtcgcg cgtgagcacg aggagcgcgt tcacgaagcc
     3721 cggcagcccg tcgcgcgccg tctcgacggc ccggtcgcgc acccagacgt aggagccgcg
     3781 gaagtgctcg aggatccgac gcgcgaggac gtccggcgcg tacgggagga ggaggctcac
     3841 ggtgccggcc cggacgagcg cgttgccgtc ggcgagcagc gggacgatct cgggcgtgtg
     3901 gtcctgcttg gccgtcgcga ggagcttctt gagcgcgccg aacgacgcct ccatcacgcg
     3961 cccgcctccc gtccgcgcca tctggaggac gtccgggaag tagctctcgt tcgggttctt
     4021 ctcgaggacc gcgagcgcgg agtagcggat ctcctcgttc tcgtcctgca ggagaggaac
     4081 cgcgaggctc gccgcgacgt ccgcgcttcc ctcgaagttc atctcgctga cggccttcag
     4141 cgcgcgcgcg cgcacggccg ggttcgaggc ggtcaggacg cggcgcgcga tgtcgaacgc
     4201 gacctgcgcg ggcagctcgc gcgcgagctc gagagccgcg aggcgcgcga agtcgtcgcc
     4261 gcgcgaggcg acctcctgga ggaagggtcc gagctggggg cccgcgaggt ccttcaggaa
     4321 gcgcatgacg gcccggcgca cggcctccga acgcgtcttc aggtgcggga cgagggcctc
     4381 gaggcccgcg cggtcgtggc gacgcttcag gatcgcgagg ccggccgtgc ggatttcggg
     4441 gacggagtcg acggccgtcc acgcgaccca ctcgacgtcg ggtttcggga gcgcctggaa
     4501 cttctcgagc aggtccttct tctggtcctc gctcgaccac ttgtgctcgc gcagcgccgc
     4561 gatgaacgcg ggttccgacc gaagcccgcg gagcttttct agcacggcgt cccaggctcc
     4621 atcgtgccgc tatcgtagcc gaccgccctc gcgcgccgcc gaggacccgg cgccggggct
     4681 ttccgatgtt acgaaaagtg tcacctgaca ggacggcttc gccgccgcgg aacgtccgag
     4741 caccggaaga aggcccttgt gcgacaatga gttgcgattc ccggtcagtc cccggcacga
     4801 acctcgcgta gaagggagcg ggaaaccatg acgatgagcc ttccggaacc cggctgccgc
     4861 accgtgaacg gcccctccgc gtccggcgga aggcgcggct tctcgctcgt cgagatgctc
     4921 gtcgtgatct tcatcctcgc gatcgtcttc atcatcgggg ggagcggggt cgcccgcgcg
     4981 tggaagcggc agaagctcca gagcgcctcg atggacatca aggtcctgat gcagcgcggc
     5041 ctgccggaga tgcagcgccg caacatggtc acgttcgtcc aggtcggccc gctcgtgaac
     5101 aacgcgtccg tgcgctacct tccgatccgg ctcatcggcg acgcgaacgg caacggggcg
     5161 atggacgcgg ccgcgaaccc gcccacggtc gcgtcccccg acctcctgct cgacgagtac
     5221 gacatcgtcg tgaccggcaa gtccggcgtc aaaggctcca cgggcgtctc gcaggatttc
     5281 tgtctctcca ccctggacat cacccaggtc cagtcggccc tctggtcgaa caactccacc
     5341 gactggacgg tgggccgcgc gatcatgtgc gacttccagg gtcgcgcgat cgacgtcacc
     5401 acgggccgcc agctcgccgg cgaggcgacg ctcgtcctct cgcacgtgga cgtcgtgaac
     5461 cggtcctaca tgcctccgac gcgtttcatc ctgagcatca accccgtctg gagcgtccgc
     5521 gtccgcaagc agacgaccgc gggagacccg acggattccg gggccgtctg ggtggatcag
     5581 caggggggat gagatgaggc ggacgacgcg cggatcgagc ttcatcgagg tcctgctcgc
     5641 gatgacgatc atggcgctcg tcctcgtcgg gatcctccag atgttctcgg tctctctcgt
     5701 gatcaacaag ggctcggccg cgcgcacgca gatgctcttc aagtgccagc aggtcgtcga
     5761 gaacgtccgg ctcttctacc acctcacgag gctgggctcg acgacgccgc ccggcatcac
     5821 ggagagcggg cacgtgagcg gcacggcgat gacgacgccc ggggcgcttc cggacgtgct
     5881 cggcgacgtc tcgaagtcgt tcctgcccta caaccaggcc gagtcggccg cgtcgtgggc
     5941 ctactggggg ccggccggcg cgaacgtcat ggagcaggag aacgggccct acaagatttc
     6001 gtacacggtg gcgcgtgaca acgtgcagaa cctctgggtc atcaccgtct ccgcgacgcc
     6061 gacggacgtc gcgacggcga cgcggtactt cggcgtcgac ctcaagagcg gcaagagagt
     6121 ggactatgtg gcgcagttcc cgtaaggccc tcggcctccg gcggggcctt acgctcaacc
     6181 gccgcggctt cacgctcatc gaggcgatgg tgtcgctgct gctcctcgtc atcgtactga
     6241 cggtcgcgat gacgatgctc ttccagatgc gcgccttcgc ggaacgccag cagttcttca
     6301 tgctgccgcg gcaggccgcg cgccgcgcaa cggactacct ctcgtactac gtcgcgagcg
     6361 cgtcggacgc caacgacgtc cagaacaacc cgaacgccct catcatgtat tacaacctga
     6421 acggcaacgt gctccaggcg tcgtacgaca acctcacggg ggccgagccc ggcaactcgg
     6481 tcacgacgcc gaacgtcacg acccagttcg gggacatcgg gacggacgtc atcacgctcg
     6541 tcgcgcccat caacccggcg aagttccgcg tcttccctcc cttcccgtcg ttcacgggcg
     6601 gcgccaagaa catctacgtc aacttccgcg tggggtgcgg cgggccggcc gggacggacg
     6661 acgtgacgaa catggcccag ttcaaggcgg cgacgggctt cgacggcacg aacagcgccc
     6721 tcctcatgtt cgaggacacg aacggggaat ggtcctattt ccagattccc gccgcaggct
     6781 acatcgcgtc ggactgctcg gacgtcacga cgtaccggaa cgtccacgtc cgcgccgcca
     6841 tccagtccgc cgcgctgccc gccccgccga acggcggcgt ggcggtgttc aacgatccgg
     6901 tctccctcgt cacgggtctc cagttcatct cgttccgcgt gcggaccgac ccggtcgaca
     6961 acctcccgaa gctccagcag aagatcggcc tcttcgaccc gaacacggac aacccgggag
     7021 ccgcgttcgt gaacgtcatg gagaacgtgg aggacctgca gatcgcgtac gtgtacgcga
     7081 acggcgacat ctggaacaac accgcggggc agacgatcac ccaggcgaac gcgggcgcgc
     7141 gctgcaacgt cgccgcctgc gacaaccgcg tccccacgca ggccggaccg ggcgccgtca
     7201 cgatggaggc gctcgacgtc acgggcgtca tcggcctcag gttctccatc acggcccgct
     7261 cgccgcggct ctcgatcggg gcccgccagc tcacgaacgt gaacgtcacc gacgtcctga
     7321 cgacgacgac gagccagcac ttccggcccg cgtccgagaa ccacccggtc acgatggacc
     7381 cgctggcccc cctgcggccg ctctacgacc tcttcgacca ctatcgcgcc acgtccacga
     7441 tgcttctgcg caaccgggcg ctcgggagct gacgatgaag gaacgcggca gcgcgctcgt
     7501 ctccgtgatc ctggtcctcc tcgtcctgac gatcgtgggc gtcgggatcg cctacttcac
     7561 gtcgatggag gaccggctct ccggcaacac gcgcatcgcg aaggccggct tctacgccgc
     7621 cgacgcgggc ctgcggcgag ccgaggtcgt cctctccggc ctctccgacc tctccgggac
     7681 gtgcgcgccc gtgtcgctca cgaacctcct tcagaccggc tcgaccccca cgctcacggt
     7741 gccgggcggc ggctacacgg ccatcccgct cgacctcacg ggaatctcga cgaacgcctg
     7801 cctgacgccc ctcggcatcg cgcagcagaa gttctacaac gtccccgttc ccgtcgtcgc
     7861 cgggacgccg gtcgtcgacc gcgcgacgta ctccctctac gtccgcaaca acgtcgacga
     7921 cgcgtccgga gacgccacgg tggacaacga cggcctcatc aacgtcgtgt ccgtcgggac
     7981 cgtcacgctc acggcgggga cgacgctgac gcgcatgctc gaggagcagc tcctcctcac
     8041 gacgggcggc gccttcggga gcagccagaa gaacctgaac gcgggcggga ccggcaccgc
     8101 ccagaagaag ggccgctgat gcgacgagcg ccgttccgca tccccgaacg cctccggcgc
     8161 gcctgcgcct tcctgctcct cgccgtcatg gcgttcggcg gggccggctc ggcgcccgcg
     8221 gccgcgaccg aaggattcga ccccctccgc cagatcacgg cgcagctcct ccgcgcgaac
     8281 ctcctcatcc tccaggacat cagcggctcc atgaagctcg acgtgtacgg gaacctcctc
     8341 acggaagggg aggactccgt cggccgtctc gtctggaccg tcacctgcct cgacaacagc
     8401 ccgggcgcga ccccgacgcc gacggcgacc ccgacggcca cgaacacgcc cacctcgacg
     8461 gcgacgtaca cgttcacgtc gaccgcgacc ccgacgtaca cgttcacccc gacggacacg
     8521 gcgacgtcca cgccgacgtc gacgccgacc gcgacggcga cgtccacgtc cacgccgacg
     8581 cccacggcga ctcccacggc gacgtcgacg cccacctcga cgccgaccgc gacgtcgacg
     8641 cccaccccga cgaacacgcc gacgtccacc gcgacgccca cgtggactcc cacgtccacg
     8701 tccacgccga cgtccacgcc ctccatcacg ccgacgaaca cgccgacgtc caccgtcacc
     8761 ccgacgtgga cgggcacgat gacgccgtcg tcgacgccga cgaagacgcc gacgccgtcg
     8821 agcacgccca cgtccacgaa gacgccgacg cccacgagga cgtccacgag cacccggacc
     8881 ccgacgaaga cgccgacgcc cacgaggacg ccgacgcgca cgaacacgcc gacgtccacc
     8941 cggacctcca cgcggacgaa cacgccgacg ccgacgccga cgggcacgcc gaccccgacc
     9001 gcgacgtaca cgtacacctt cacgacgacg ccgaccttca cgttcacgcc gacgccgacg
     9061 aggacgccga cgccgacgtt cacctcgacg ttcacctaca cgcccacgac gacgccgacg
     9121 cccacgaaca cgccgacgtc cacgatcacg ccgacgaaca cgcccacgac gacgggcacg
     9181 aacacgccca cgaacacgcc gacgcccacg aacacgccga cctcgacgat cacgccgacg
     9241 ttcacgccga cgctcacgtt cacgtccacg ttcacgtcca cgccgacgtc cacgaagacg
     9301 ccgacgccga cgccgacgaa cacgttcacc gtgacgttca cggccacggt gacgccgacg
     9361 tccacggaca cgcccacggt gacgccgacg aataccccga cgccgacgaa gacgccgacg
     9421 gggacgagca cgcccacgaa gacgccgacg ccgtctccca cgttcacgaa cacgccgacg
     9481 tcgacgccca cgtggactcc gtccatcacg ccgacggcca cgaacacgta tacctacacg
     9541 tcgaccgcga cggccacggc cacggacacg ccgacgacga cgcccacgcc gacgatcacg
     9601 ccgacgttca cgaagacgaa gtcgttcctc gatctgccgc gcgacccgct cgtgaggccg
     9661 ggggagcggt tcgaggagcg catcgcgtcg gcgcgtccgg tgcagacgcc ggccccgaag
     9721 tggggcctcc cgcagcccgc cgcggtcgtc gcgatggccg gcggcggagc cgcgcggttc
     9781 ggcctcgcgg cgctcctcgc gccggcgacg ccgacgccga cgccgttccc ctccggacag
     9841 gccggctgcc accgctggaa atacgcgctg cactgggacc gcccgtcgcg cctcgcgacg
     9901 ctgaagaacg cgctcggcaa ctcggtcacg atccacaaga actacaaggc gcctggagcg
     9961 ggcacgcagg acgactggcc ggacccgatc acgcacagcg tcgactggac gggggcgccg
    10021 aacgcgggcg cgtgggactg gaagaacccg cccgcggccg cggccacgtc gttcccgtac
    10081 gaccacgact gcgacgactt cgtgagcggc ggcccggcgt gcggcgcgcc caagccgggc
    10141 gaccccgacg gcgaacaccc cgggatctgg tacacgatcg gcccggccga cggcgccggc
    10201 gagatcgtgg actggtcgtg gacgctcaac tacggctacg gcaaggcgcc cggcaacgac
    10261 atcatctacg gcctcgaccc ggggccgccg ctccccgtct acgacctcac ggccgaggcc
    10321 gtgacgggca actcgatccc cgggatcacg ggaacgccgc tcgcgttccc ggccgggtgg
    10381 acgacgcgcg gcggtgtgcc gatcgcggcg gccgcgaacc agccggcctg gcagacgctc
    10441 ctgccgtcgg acgtcgtcgg cacgacgcgg aaccaggtca actggggtct catgacgttc
    10501 tccgacgtct cggtggaccc cctccagtcg aagtggtgca cggactcgct gaactacgac
    10561 gtgaaggtcc cgatcgtgtc caacgacacg ggcgacgtca cggcgatcga ggaatacctg
    10621 cgtctcaagt actactcgag cggctcgcac ccgggcctgt cggccgcggg cggcacgccg
    10681 acaaagcacg cgatcgagct cgcaggcgcg tcgctccgga cgtcctggga caccgacccg
    10741 aagcagcgtt gcgcgcgccc ctggggcgtc atcctctgca cggacggcct ctcgaacacg
    10801 tgcaacaccg gcacgactcc ggacgccgaa tgggtcgcgg cggccgggaa cacgccgtgt
    10861 gagaacgaca ttccgggcac ggacttcgcg aactacccgc ccggcgcggc cgaagccgtc
    10921 tacaactcga tcatgacggc gggcggaggc tcggcgatca tccggccgcg cacgtatgcg
    10981 atcggcatcg cgcccgaggt cggccgctgc gagctgaacc gcgtcgcgta ccgcgggcgc
    11041 accgacgcga gcgcgcccaa gaaggacgcg ggcttcctcc tctacgaccc gaccgagatt
    11101 ccgccgccgc ccaagggcga catcgacctg ccgcacgtcg acccgctcac gaacgaccag
    11161 agcggtccgg gcgcgccgcc cgacccgaac cacttccggt tcgaccagtc tccggcgaag
    11221 acgggcgact acgcgttctt cgcgctcgac acgaagtcga tcgtgaacgc cttcctcacg
    11281 atcgtcgcct cgacggcgac gggcgactac tccacgagcg ccccggtgtc cggcgcggcc
    11341 gttgcggcgg gctcgatcgt cctgctgccc tcgacggagt acccgtcctg gcgcggccac
    11401 ctgcgcgcgc tcgacacgac gaaggtgccg gcgtcggtgg gctacgagaa gtgggatgcc
    11461 ggcgacgtgc tcctgaaccc gacgcagccg tggcatccga ccgtcgcgac gcgctccatc
    11521 tacacgtggg atcccacgac cggggacctg atccccgtcg acacgacgcc cgcgaacgtc
    11581 gcgaagatgg cggcgctcgc aggggacccg gccttcaccg agaacgtcgt ggacttcatc
    11641 cgcggcaacg acggcaccct cacgaacacc aaacgggcat ggatgctcgg gccggcgatc
    11701 aactcgacgc ccgcggtcgt cgggccgccc tacctctaca agcagaccgg caacgtcgcg
    11761 gaccacaagc cgttcgaggc gacgtacgtg tcgcggcgcg cgctctcctg ggtcggcgcg
    11821 gacgacggaa tgctccacgc gttcgacttc gacgacggga ccgagatcct cgcgctcctg
    11881 ccgccgaacc ttctcgcgaa ccagatcgcg ctctacaaga acgtcccgtc gggcgagtcc
    11941 gacacgggcc agaagctcga gctctcccag cacatctggg gcatcgcgaa ctcgttccgg
    12001 ttcgcggacg tctggttcgg gacgggctac aagaccgtcg gcatcctcac cgagggcccc
    12061 gcgggagaca tggtcgccgc gatcgacgtc acgcatccgt accccgggcg cgccgctttc
    12121 gggcccaacc ccgccatccc ggccgatccg aactacgacg ccgcgaagcc cgtcgagatc
    12181 ctctggacga agaacgcatc ggactacgcg gggctgcacg gcagctggag cctctccgcg
    12241 atcgcggcgg actccttctc gacgagccgg atgttcttcg gcgcgggcat caaccccgcg
    12301 agcctctaca acgcgcaagc ggacgcgacg ctcttcatcg tcgatccgac ggacggcacg
    12361 ctgaaccaga cgaagacgat cgcgctgagc ggaacgccga gcccgctcgt gggccagcag
    12421 acgttcgcgg actcggtctt cttctcgacg aaggtgtcgg gctacctgcc cgacaacgtc
    12481 gcgaacctcg aggtccaggc cgacacgaac ggtcgggcct ggttcacgtg gggcgacttc
    12541 gccggcgcct cgacgacgac gaaggtgggc atcgacctga actacgcggc cgtgccggtc
    12601 gtctccgggt cccagactcc ggtcccgcag ccgatctact actcgccgtc cgcgacgggc
    12661 cagggcacga cgggctgcca gatctacgcc ctcgcgagcg gcagcctcta cgagacgagc
    12721 ccggcggtct cgggatggaa cgtgaaccgc agcggcgcgc ccctcgcgcc gttcccgaac
    12781 acgctgccgt cgttcacgcc gtacctctac atcggggtca acccgaagaa gatcacgtcg
    12841 ggcaccttcg cctcggtgcc gctcggcaag gatcctctcg acgtcttcat gatcaagcag
    12901 cgcatcggcg gcgaagtgcc cgaggcgatc gacctgccgc cgggcgaccc ggctctcacg
    12961 gggacgccgc ctctctcgaa gctcgggctc cacacgcagg tcacgtcctc gccgatcatg
    13021 atcgtggacc agacgggcaa gagcgaggag caggccctgt tcctgctcta cgacccggac
    13081 aacgggtgca acggcatctc gtacgtcgcg atcctcacgt tcaagatccc ggcgaactgc
    13141 ggccagccgg ccatcaccca gattcaggtc tacggcgccg gcccgggcgc ggcgagcggt
    13201 ttcacgctca cgtcgaacaa gctcttcgcg tcgaagtcgg gcgtcggccg cggccagtcg
    13261 gccggcctgc aggaagtcaa cgtgcccgtg aacacgctcg cgggcgcgcc ttccttccag
    13321 ccggtctggt ggagggacgt caagtaaggg cccggcgccg gtcccgctaa gatcccgcca
    13381 tggacgcggg accggcccgc cggcgctccg ccggcttctc actcgcggag ctgctcgtga
    13441 cgctcgccgt cctcgggctc gcggcctggc tcgttctccg cctcgtggac cggcgcacgc
    13501 gcgtcgccgc ccccgagggt gcgggctccc gcacggccag cgtcggcgag gcgtccctcc
    13561 gggcgatcgt gaacgacgtc cgcgccgccg gcaccggagg cctctctgcg gccgacgcgt
    13621 tccgccccgt cgaggacaac acgacgtccg cgggccgtta cctctaccgg accgcggccg
    13681 gaggaaccgt cgtcgtgcgg aacggaacgg accagctcgg gctgcgcggc gtcatccgtt
    13741 cgcctctcgt cgcgctcgcc ccccgcgacg cggtcacgga cgcgctcctc tcggaccgcg
    13801 tacgcgcgcg gccggatgcc gcggccgtgc accttccggc cggaaaggcc gccggggcgg
    13861 tgcgggcgcg actcgccgag gcggcgccgc cgcgcaaggc gttcttcctg ctccgcgacg
    13921 ccgcgggccg ctgggccgtc ggacgggtcg tgtcggcggc cgagggcctg ccggaatcgc
    13981 cgctcgacgt cgtcctcgac ttcacggaca gggacgcccg ggcgctcaac cacggaggcg
    14041 atccggacgc ggcctcgcgc ctcggggacg tgtcggccgg cggcgtcttc gacgacctcg
    14101 tctggttcgt cgctcaggga gaggagggga agcctccgga cttcgtgatg acgaacgacc
    14161 ctgcgtctct cgcgttcccg cacccgtatc tcgccgccgc gacgggcgtc ggggccgacc
    14221 gctgggacgt ccgccgcgcg ggagaggacg tcgaggacct gcaggtcgcg tggggcttcg
    14281 cgggcccttc ggggtcgatc gcctggcgcg gcgacgccgc ggactctccg gcgccgctcg
    14341 ccggcgagct tgcggacgcg gcgggacggg cccggatcaa ggcgctcaag atcgcgctct
    14401 ccgtgaagtc cgcccaccgc atcctccgga gcgcgggcgc tccggccccg gaattcacgc
    14461 gcctgttcaa cgcggccccc ttcgggaaga tcccgggcgc cgcgccggtc ggctgggatg
    14521 cggtcccgga gcggcgcgtg agcttcgacc gcgagacccg cgaagcggtc gtcgcgccgc
    14581 ccgcgctgct cgaagcgggc ccgtgacggg cgcccgggtg taaaatcgcg gatgcggttc
    14641 gtacaaaggg agggtccatg cgacggctac tgatcgccat ggcggtcttc gcggtcgggg
    14701 cgggtgcggc cgcggccgac gagatctggc tcaaggacgg ccgctcgatc acgacgaaga
    14761 aacccgtcgt gacgaagggc tcgaacgccc tcatcacgac ggccgacggc gtcctcgtct
    14821 ccgtccccct ctcggagatc gaccaggcga agaccgcgga ggcgaaggcg cgggcggccg
    14881 cgcccgcccc ggcgcccacg ccgaatctca tgaagcccat gacgccggcc gaggccgcgc
    14941 gccagaagag cgcgcgcaag gccgtcgtca cgctgaccga cgattccgtt gcgcacgggt
    15001 tcggggacga ggcgggcgag cgcaaggccg ccgacggcga gggaaaggtc gaggtcgcga
    15061 acacgaccgc gacgcgcggc aaggacggct acgcgttcac gggtacggtg atcaacagcg
    15121 gcgcgggccc cgtgacgggc gtttccgtga cgatcgagtc cgtcggcagg gacggcaaga
    15181 cgttctcgtc gacgttcgcg tccgtcgcca aggacgcact cggcccgggc gagaaatcga
    15241 cgttcacggc gagcgcgccg ctcgagggcg aggccgcctc gttccgctac atgccgcgct
    15301 ggcaggtccc gatcccggag aagacggaag acgagaaggc gaaggcccag ccgaccgtcg
    15361 ccgcccaggc cgagcaggtc accgacgacg tgcgccgcg
//
LOCUS       DAOMFY010000068        15242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1195_length_15242_cov_8.463949, whole genome shotgun sequence.
ACCESSION   DAOMFY010000068 DAOMFY010000000
VERSION     DAOMFY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15242
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1195_length_15242_cov_8.463949"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLB01_07605"
     CDS             <1..767
                     /locus_tag="PLB01_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQQ77206.1"
                     /translation="ASSPKRTPEDMCSRSRIGVRPYSLPLNPATYFSGGSSTDCNAPS
                     ATARLLAPVCRAVAYEKGDEVFREGAPAEDLCFVVLGRVKVVKAGPERDVILGLFGPG
                     EPVGAVAVFEGKRFPASAVALEPSTVLRIPERAFFAAIDAHPEMTRSLLQGLMVRQFE
                     ITRRLADASAPVERRIARLLLTLAARLGRAEGGPVTIPLALSRLEIAELTGTTVETAI
                     RVMSRWGKENLVATTPDGFVVHDEAALRQIAYADAG"
     gene            complement(776..1321)
                     /locus_tag="PLB01_07610"
     CDS             complement(776..1321)
                     /locus_tag="PLB01_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ77207.1"
                     /translation="MAHPLDLAPAADEYAEYYGRYVSRVPRGDFFALLRSQADEVASV
                     FGALAPAQADFSYAPGKWSPKEVLGHLTDTERVFAYRALSVGRGDPSSLPGMEQDDWM
                     ATADFGRRAIGDLLAEWRAVRAATVTLAEGLPAEAAMRRGVASEKPVSVRALLHIVPG
                     HTTYHLEVLRERYFGAADWPT"
     gene            complement(1332..2579)
                     /locus_tag="PLB01_07615"
     CDS             complement(1332..2579)
                     /locus_tag="PLB01_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77208.1"
                     /translation="MLKPVELEIELFCLGMRIDESCHVDEDGRRISRTRAGLGSGLEL
                     LLPSPRKKIWVNVPVVEPFAAKSPLLLVKSGGAYAVTDTRTGDHYPVEVPPEPEWYSW
                     KTKTGAEMSRIGVLQGTYLGIYVSNSCLYWNLPASPACAFCTTGRNVGEAEESRKKVA
                     DVVEVAKAARDHSGSIFTHLNTGYHFEDIDKHESLHGLRQCEPFVKAIRAEVGGFIGV
                     QAVPVPRRKFQEYDDLIAAGTDHFSFCYEFEDAETFARLCPGKAQTVGQQAFFDAMEY
                     TAKKLGPGKVSGEIIAGPEPLGATKRAIDRIVNAGAFPTVCIFRPTLGSAMENVDPPK
                     PDDMKEVFAYLWQAVTRVGLPVGILPGIQVSLVVQPEEAAELVPPTLGSRFYETKLAA
                     LRTLAKPYVAWKRRPRGGVAAGA"
     gene            complement(2587..2790)
                     /locus_tag="PLB01_07620"
     CDS             complement(2587..2790)
                     /locus_tag="PLB01_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77209.1"
                     /translation="MSKGRSEDEQKAVETILWALGHKLQRLRDEYTMSAQRGEEPAGI
                     AKGLEIALREVNDLLPRRKRLKG"
     gene            2821..3561
                     /locus_tag="PLB01_07625"
     CDS             2821..3561
                     /locus_tag="PLB01_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQQ77210.1"
                     /translation="MPVLAVLVRTHNPGNLGAAARAARNFGAELALLGPRTDPAHPDA
                     ISFASGAEELLRKVKILGSFDELAARADSVVALSSLRGRVSRGLPPPITFKEISSSLR
                     HGRRVALVFGPERGGLTTAELQACDARLTIPTAPDLPTLNVAQAVVATLTLLYPFEAS
                     SRRRRETAPPGEQPAPTKDVRRLLSSLKETLSSAGYPGRGHSREVIAEIESFVKRGKP
                     TAREVTLLLGALAAVRRGLGVSPSKPRG"
     gene            complement(3536..3763)
                     /locus_tag="PLB01_07630"
     CDS             complement(3536..3763)
                     /locus_tag="PLB01_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HQQ77211.1"
                     /translation="MKDPVQRYSKDGLTVTFDPRVCTHSGVCVRGLFDVFDVARRPWV
                     NLDGAPAEKVVEQVKRCPSGALQFSLGASKG"
     gene            3938..4780
                     /locus_tag="PLB01_07635"
     CDS             3938..4780
                     /locus_tag="PLB01_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77212.1"
                     /translation="MNTKRLASILVPLALAAAALPASALSVTSVSLSVTPTHSDGPCP
                     VKFTFTGKVTLNGRGEFRYKWERSDGAVDTAGPHTAVYDGTHATIVTDTWTLGAPGAP
                     FHPFNGSVTLHVLAPNDTSSKPAVFTLDCGAPPPTIVPGAIGRPTLPAGAGPVIHVAR
                     PVFDPAVLLLPAVQVPQAFRGADGKTQPAGPYDLKVSQGAQGILIGLLRNGKLAGEAN
                     GRFVPPPDADRKAGAHDFHFDASSKVSFGGGGGAGRISCSINQHSGPMLLPYIEFHLP
                     AVQK"
     gene            complement(4793..6709)
                     /locus_tag="PLB01_07640"
     CDS             complement(4793..6709)
                     /locus_tag="PLB01_07640"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HQQ77213.1"
                     /translation="MATTYTARDITVLEGLDPVRKRPGMYIGGVDTRGLHHLVNEIVD
                     NAIDEVINGFATRLDVTLFADGKEIEVTDNGRGIPVDVHPKYKKSALELILTTLHAGG
                     KFEQGNYLHSGGLHGVGSSVVCALSSEMTVTVKRDGHTWQQTYAKGKATSKVAKISGG
                     EKVRGSGTTVRFRPDVEIFGKLAFDAAEIRDRLEAKTYLHRGLRVVFTDETKKPAVRE
                     ELSHEGGLADWLMHVVAQRGKAATHAGAFVLERPEDPRLELALVWTEATDEHVRSFVN
                     GVSTPSGGTHENGLRSGVTKAVRNYVETHELLPKGVSVTAEDIREGLVVLLSAYVLEP
                     QFQGQTKDRLNNPEMAAAVDGAVRPALEKWLNENPTLAEQIVGRIVLAARAREASRAA
                     QQVARKTAVSHRLNLPGKLADCSSTDPGSSELFIVEGDSAGGSAKQGRDRRTQAILPL
                     RGKVLNAEQANAAKVTGNRELSDIVSALGCGVGADFDIEKLRYGKIFLLMDADADGHH
                     ISTLLLTFFWRHLPKLIERGHVFLAQPPLYRIDAARETHWALDEKERDAILAKLPKNV
                     KPEIMRFKGLGEMMPEQLKATTLDPERRRALKVTVPSVLEAEKALGELMGKDASLRYK
                     FIMERAREADDLDV"
     gene            6780..7778
                     /locus_tag="PLB01_07645"
     CDS             6780..7778
                     /locus_tag="PLB01_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77214.1"
                     /translation="MSAFLARAFDFVLLVLDRTGAGAKIATLPFFPAAMPLASRAAWA
                     LALLGAAVLLLYSSLAAGPDRRVAAFRVLTLLGGCLFPLLAARLVPAGFARPFGGAVL
                     TVTFTAGLVHRALAGLRRESAASRFLEGARWLLEVAGILLFVAAALVFFGEGRVLGPA
                     ALWGLLCLRLSIADLLDPSRLAKETGLTSSAAKDLKAARRGRTPAAHRGARFLSGVFK
                     LGLAVAWVFLPLLAAVAPGEIARGEWPPAARVLLAYPAAALFLTGILLLLGAMKRLPS
                     SPFEAARGAIVGAGTLLWLWVVFDRASFGEAVQILPGLYVAETLAGFLFGAAARGR"
     gene            7802..9160
                     /locus_tag="PLB01_07650"
     CDS             7802..9160
                     /locus_tag="PLB01_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQQ77215.1"
                     /translation="MPDTVSGREAPVLARRIGFWSAVAVVIGSTIGSGIFRSPAGIAD
                     KLPGPLPMLAVWAAGGVFALCGALTLAEVASALPQTGGMYVFCRDGWGRLAGFLFGWG
                     QFSMIRAASLGAIAITFSEYFFRVTGQNPQAPDKVMAVRWFAAFAIALTAGFNIFGVR
                     FASNVSNVTVIAKYGGLVFIVLVALFVGLPKTGGNFSPAVPAGSFHVAAFGLALVSTL
                     WAFDGWADLAYNAGEVKDPQKNLPRALIGGTLLVVAIYLAANVAYLAVLPIEKIRVSK
                     LVAADVAQVVLGTAGAAFVSITVMVSTFGTLNTVLFTSPRVFFAMAADRLFFAPVASV
                     HPRFGTPWISISMTAGLGIVFVLSRNFEQLADAFVTAFLPFYALAVASIFRLRKKPGY
                     APTFKVPLYPVLPVVFLASVLYLLGNAIVQESSRMSTLAVLGVVAAGIPVYYLTVGRK
                     TA"
     gene            complement(9157..9807)
                     /locus_tag="PLB01_07655"
     CDS             complement(9157..9807)
                     /locus_tag="PLB01_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HQQ77216.1"
                     /translation="MASASAVLYLHGLASSPLGRKRAVLEDRLGPDGIEIVAPDLNVP
                     SFRKLDFSEMVRAARTACDRARPRVIVGSSLGALVALSLAESFPLLSPPPPLVLVAPA
                     LGFHERWKEKLPESDTFDLFHHGEERQLPIHRRFFEEMALVRVDDAPPPVPVSVVMGT
                     DDETVPYALVADAWERWEASGRLVKGSRFHRVAGGDHGLVAHGDEIESAVRERLAG"
     gene            10040..12790
                     /locus_tag="PLB01_07660"
     CDS             10040..12790
                     /locus_tag="PLB01_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ77217.1"
                     /translation="MLLVTVLATAAWAGETGSVSGVVKDGGGLAVPGATVKITGAQAP
                     RTTVTNASGAFKFDVLLPGDYVVTADLKGLGSASQKVRAYVDNDAQLTLILVSSAKAE
                     VVVTGAAAEIDKTSSESNFNYTDNTLKDLPLSRTYQGVIKIIPGAPTDTSGQGFVSIS
                     GGTRQDNKYLLDGVNVTNPGYGWLGVDTNQLDIADFNVKKAGISAEFGRTSGAMINAV
                     TKSGSNQIAGAVQVNLSPSSFQANRSFDVQSDTDLYNGQANFGFPIIKDTLFGYVSGA
                     YYDAKTSGQSATVGGVTTTQPDSKSHNGDYFGKLTYFAGQSLLVNAGFRALPAKSTDQ
                     FGSIYDLPSAAQGADTTNYVGNLSVDWFASKNTVVEAKYVYLEQNDTSAAQTVLAPFQ
                     PAVINPKNLGAYGAYADPARNGGNVGVPEFAETGEKYKRSEIKATLSQYFDGGSMQHA
                     FKVGGGYEDAQMDTVRATNGWGIWATGQTCPASVCGTSVSGTVRARYYTSQPVQYSLA
                     RTYSAFIQDTITMKNLTVYLGVLVNKDDFAQLCKTGNVCGSSTTAPNTSDVRYNFMTF
                     DWSQEIQPRLGITWNTNLLPSDKFYATYGEYAGVDQKSTARSFAPYRIRQDQAYFNGT
                     TGVFLGSQYRGSSGGKVIPPDLKPPKYEEFSVGYSAALTKDISVDAYYQYRNLKNAFE
                     DAPIDVNNYFGSFQAKNFFNARRTYDALTIDIQKRYSNSWYADMNVTFSRLYGNWDED
                     YGIGLFNTSSFLEDEPGWNSADPNRYGHLGQDRPIIFKLMGSYDLPMGFVVGGFLRVQ
                     SGTPWEARGGTPSTTSGRYLEPAGTNRLPTWTTVDLLLAYNLKLGGNMAVRLEARVAN
                     VFDTQTVLSVNSIKYNDGYVDGNPAGNLGWQQTNQPNANFGNATSWSSPRRFTLTARL
                     DF"
     gene            complement(12895..14781)
                     /locus_tag="PLB01_07665"
     CDS             complement(12895..14781)
                     /locus_tag="PLB01_07665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ77218.1"
                     /translation="MKRLFLLLAATLAAKTAVLAQLGSHPLLQPYGEIDSGVYARLAH
                     RIAGGDLLLRGDAVPYFVSPLYAYFLAATGPSFPVARVVQILLGTAAVALVFLTARRL
                     FGERAALAAGVLYALAGVVTFHEVLILQAAIDPFLTALFLYLLARAVRSEEEEEEREK
                     ISLKVKRGEARTGTRVRLGEIVAAGAAGGFLSLNRPNALLCVAAVAVALALFTFKENP
                     LRRVWIASAFVAGAAVTIAPFTLRNLFVSREFVLVSSHGGLNFLVGNGPGADGVYRQL
                     PGITPDIGGQAADTKRVAEAAEGRALTTGEVSSHFAREAFRWIRANPGSAASLFLRKI
                     RFTLSGDEAPLNFSFPWYREKSLALKLLFVGPGLLVPLAGAGLVFGLLGAAGAASERR
                     PALLVWATFIPAYVFAVAAFFVATRYRLPLLVALSPLAGAAVTQAGEAWRAKGARLAV
                     AAAAATVLAIVSIWPTGLWDGAPDEEMHLVLWEIEKGEAGAMAHAESAAQAHPDPGLV
                     WLRAGRSFAAAGKPEAAAAAFARSLASSTPKPEAAVALSALLEQRGLSRMKGGDVAGG
                     RADFEEAVRLDAANAAARLNLAVACAMAGETDRARGLAREALVLKPDYEKAAAFLKAL
                     DGAK"
     gene            14823..>15242
                     /locus_tag="PLB01_07670"
     CDS             14823..>15242
                     /locus_tag="PLB01_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQQ77219.1"
                     /translation="MSAFWIGVDGGGTRTRAVVAGEDLVPMGRGASGPANAATRPLPL
                     VVETILEAAGDAATSAVVSIRRAERVGVGLAGVESAGLVQPLTAALEEHFGAGRVFVT
                     TDARIALAGAVAGDPDGPGVVLIAGTGAIAFGRGTDGA"
BASE COUNT         2246 a         5524 c         5070 g         2402 t
ORIGIN      
        1 cggcttcctc gccgaagcgc acgcccgagg acatgtgcag caggtcgcgg atcggcgtgc
       61 ggccgtactc gctgcccttg aacccggcga cgtacttttc cggcgggtcc tcgaccgact
      121 gcaacgcgcc gtcggccacg gcgaggctgc tggcccccgt ctgccgggcc gtcgcctacg
      181 agaaggggga cgaggtcttc cgcgagggcg cgcccgccga ggacctctgc ttcgtcgtcc
      241 tcggccgcgt caaggtcgtc aaggccggcc ccgaacgcga cgtcatcctc ggcctgttcg
      301 gccccggcga accggtcggc gccgtcgcgg tcttcgaggg caagcggttc cccgcctcgg
      361 cggtcgcgct ggagccctcg acggtcctgc gcatccccga gcgtgcgttc ttcgcggcga
      421 tcgacgcgca ccccgagatg acgcgcagcc tcctgcaggg cctgatggtg cggcagttcg
      481 agatcacgcg ccgcctcgcc gacgcgtcgg ctccggtcga gcgccgcatc gcgcgcctcc
      541 tcctcacgct cgccgcccgc ctgggccgcg ccgaaggcgg tcctgtcacg attccgctcg
      601 cgctctcgcg cctggagatc gccgagctga ccgggacgac ggtcgagacg gcgatccgcg
      661 tcatgagccg ctggggcaag gagaacctcg tcgcgacgac gcccgacggc ttcgtcgtgc
      721 acgacgaggc cgcgctccgg cagatcgcgt acgccgacgc gggctgagag agccgctacg
      781 tcggccagtc cgccgcgccg aagtaccgct cgcgcaggac ctcgaggtgg tacgtcgtgt
      841 ggcccggcac gatgtggagg agcgcccgca cgctgacggg cttctccgag gcgacgcccc
      901 gccgcatggc cgcctcggcc gggaggccct ccgcgagcgt gaccgtcgcg gcgcgcaccg
      961 cgcgccactc ggcgaggaga tcgccgatcg cgcgccgccc gaaatccgcc gtcgccatcc
     1021 agtcgtcctg ctccatgccc ggcagcgagc tcgggtcgcc gcgccccacg ctcaaggcgc
     1081 ggtacgcgaa cacgcgctcg gtgtcggtca ggtgcccgag gacttccttc ggggaccatt
     1141 tcccgggcgc gtacgaaaag tccgcctgcg cgggcgcgag ggcgccgaag accgaggcga
     1201 cctcgtccgc ctggctccgc agcagggcga agaagtcgcc gcgcgggacg cgggaaacgt
     1261 agcgcccgta gtactcggcg tattcgtcgg cggcgggcgc gaggtcgagc gggtgggcca
     1321 cggcgcgccc ctcaggcccc ggcggcgacg ccgcccctgg gccggcgctt ccacgcgacg
     1381 tacggcttcg cgagcgtgcg cagcgcggcg agcttcgtct cgtagaagcg cgatccgagc
     1441 gtcggcggaa cgagctcggc ggcctcctcg ggctggacga cgagcgacac ctggatgccc
     1501 gggaggatcc cgaccgggag ccccacccgc gtgactgcct gccagaggta cgcgaagacc
     1561 tccttcatgt cgtccggctt cggcgggtcg acgttctcca tcgcgctgcc gagcgtgggc
     1621 cggaagatgc acacggtcgg gaacgcgccc gcgttcacga tgcggtcgat cgcgcgcttc
     1681 gtcgcgccga gcggctcggg gcccgcgatg atctcgcccg aaaccttgcc gggccccagc
     1741 ttcttcgccg tgtactccat cgcgtcgaag aacgcctgct ggccgaccgt ctgggccttg
     1801 cccgggcaca gccgcgcgaa cgtctcggcg tcctcgaact cgtagcagaa cgagaaatgg
     1861 tcggtcccgg ccgcgatgag gtcgtcgtac tcctggaact tgcggcgcgg gacgggcacg
     1921 gcctggacgc cgatgaaccc gccgacctcg gcgcggatcg ccttcacgaa gggctcgcac
     1981 tgcctcaggc cgtggagcga ctcgtgcttg tcgatgtcct cgaagtgata gcccgtgttc
     2041 aggtgcgtga agatcgagcc cgagtggtcg cgcgcggcct tcgcgacctc gacgacgtcc
     2101 gcgaccttct tgcgcgactc ctcggcctcc cctacgttgc ggccggtcgt gcagaacgcg
     2161 caggccggcg aggccggcag gttccagtag aggcacgagt tcgagacgta gatcccgagg
     2221 tacgtgccct gaaggacgcc gatccggctc atctccgcgc ccgtcttcgt cttccacgag
     2281 taccactcgg gctccggagg cacctcgacg gggtagtgat cgcccgtgcg cgtgtccgtg
     2341 accgcatagg cgccaccgct cttgacgagg aggagaggcg acttcgccgc gaacggctcg
     2401 acgaccggga cgttgaccca gatcttcttg cggggggacg gcagcagcag ctcgagtccc
     2461 gagccgagac cggcgcgcgt gcgcgagatg cggcggccgt cctcgtccac gtggcaggac
     2521 tcgtcgatgc gcatcccgag gcagaacagc tcgatttcga gctcgacggg tttcagcatg
     2581 gagtttctat cccttcagtc tcttgcggcg cggaagaagg tcgttcacct cgcgcagggc
     2641 gatttcgaga cccttcgcga tgcccgcggg ctcctcgcca cgctgggcgc tcatcgtgta
     2701 ctcgtccctc aggcgctgga gcttgtggcc cagggcccac aggatcgtct cgacggcctt
     2761 ctgttcgtcc tcggatcgtc ccttcgacat gggccggatt ctgacccaag atcgcccccg
     2821 atgcccgttc tcgccgttct ggtccggacc cacaaccccg gcaacctcgg ggccgccgcc
     2881 cgggcggcga ggaatttcgg ggcagagctt gctttgctcg gaccgcgcac cgacccggcg
     2941 catcctgatg cgatctcatt cgcatcgggt gccgaagagt tgcttagaaa ggtgaagatc
     3001 ctcggctcgt tcgacgaact cgctgcgcgg gcggactcag tggtcgccct ctcgtcgttg
     3061 cgcgggcggg tttcgcgggg gctgccccct cccatcacct tcaaagaaat ctcctcttct
     3121 ctccgccacg gccgccgcgt cgcgctcgtg ttcgggcccg agcgcggggg gctgacgacg
     3181 gcggagctgc aggcgtgcga cgcgcggctg acgattccca cggcgcccga tctgccaacc
     3241 ctgaacgttg cgcaggccgt cgtcgcaacg ctcacccttc tttacccttt cgaagcatcc
     3301 tcgcgccgaa ggcgcgaaac ggcgcctccc ggcgagcagc ccgcacccac gaaggacgtg
     3361 cgccgcctcc tctcttccct caaagaaacc ctctcttcgg ccggctaccc gggccgcggc
     3421 cattcccgcg aggtcatcgc cgagatcgag tcgttcgtga aacgcgggaa gcccacggcg
     3481 cgcgaggtca cgctgctcct cggggcgctc gccgcggtcc gccgcggact cggcgtcagc
     3541 ccttcgaagc cccgaggctg aactgcagcg cacccgacgg gcagcgcttc acctgctcga
     3601 cgaccttctc cgcgggagcc ccgtccaggt tcacccacgg ccgcctggcg acgtcgaaga
     3661 cgtcgaagag gccccggacg cagacgccgg agtgcgtgca gacgcgggga tcgaacgtca
     3721 ccgtgaggcc gtccttcgag tagcgctgga ccggatcctt catgagcctg attctccctc
     3781 acaagccgcc ggaatcgaga atcggtgacc cggctcaaca cgtcaccccg gaccgcgcat
     3841 ctggcgctcc cggaccgctc ttcgtcgcgg gcgggccagc cgaaatcctt gccctcgccc
     3901 gcgcccggaa gcatcttgac gtgcgaagga gcgttccatg aacacgaaga ggctcgcctc
     3961 gatcctggtc ccgctggccc tggcggccgc ggccctcccg gcttcggccc tttcggtcac
     4021 gtccgtcagc ctttcggtca cgcccaccca cagcgacggg ccctgccccg tgaagttcac
     4081 gttcacgggc aaggtcaccc tcaacggacg aggcgaattc cggtacaagt gggagcgcag
     4141 cgacggggcc gtcgacacgg ccggcccgca caccgccgtc tacgacggaa cgcacgcgac
     4201 gatcgtcacc gacacctgga cgctcggcgc gcccggggcg ccgtttcacc ccttcaacgg
     4261 ctccgtgacg ctgcacgttc tggcgccgaa cgatacgagc tcgaagcccg cggtgttcac
     4321 gctggactgc ggcgccccac cgccaacaat tgttcccggc gcgatcggcc ggccgacgct
     4381 ccccgcggga gcgggccccg tcattcacgt cgcgcgaccg gtcttcgacc cggccgttct
     4441 cctcctcccg gcggtccagg tcccgcaggc gtttcgcggc gccgacggga aaacgcagcc
     4501 cgccggcccc tatgacctga aggtgtcgca gggcgcgcag ggaatcctga tcggcctcct
     4561 gagaaacggg aagctcgccg gcgaggcaaa cggcaggttc gttccgccgc cggacgcgga
     4621 ccgcaaggcc ggcgcgcacg acttccattt cgacgcctcg agcaaggtct cgttcggcgg
     4681 cgggggcggc gccgggagaa tctcctgcag catcaaccag cactcgggcc ccatgctgct
     4741 gccgtacatc gagttccacc tgcccgcggt ccagaagtag cgggccaggc cctcagacgt
     4801 cgaggtcgtc cgcctcgcgc gcccgctcca tgatgaactt gtagcgcagc gacgcgtcct
     4861 tgcccatgag ctcgccgagc gccttctcgg cctcgaggac gctcggcacg gtgaccttca
     4921 gcgcgcggcg gcgctcgggg tcgagcgtcg tcgccttgag ctgctcgggc atcatctcgc
     4981 cgaggccctt gaagcgcatg atctcgggct tcacgttctt cgggagcttc gcgaggatcg
     5041 cgtcgcgctc cttctcgtcg agcgcccagt gcgtctcccg cgccgcgtcg atgcggtaga
     5101 gcggcggctg cgcgaggaag acgtgcccgc gctcgatcag cttcggcagg tggcgccaga
     5161 agaacgtcag gagcagcgtc gagatgtggt ggccgtccgc gtcggcgtcc atcaggagga
     5221 agatctttcc gtagcgcagc ttctcgatgt cgaagtcggc gccgacgccg cagccgagcg
     5281 ccgagacgat gtcggagagc tcccggttgc ccgtgacctt cgcggcgttc gcctgctcgg
     5341 cgttcaggac cttcccgcgc agcggcagga tcgcctgcgt ccgccggtcg cgcccctgct
     5401 tggccgagcc gccggccgag tcgccctcga cgatgaagag ctccgacgag cccgggtccg
     5461 tcgaggagca gtccgcgagc ttgccgggga ggttcagccg gtgcgagacg gcggtcttgc
     5521 gcgcgacctg ctgggccgca cggctcgcct cgcgcgcccg cgccgcgagg acgatgcggc
     5581 cgacgatctg ctcggcgagc gtcgggttct cgttcagcca cttctcgagc gcggggcgga
     5641 cggcgccgtc cacggccgcc gccatctcgg ggttgttcag gcggtccttc gtctggccct
     5701 ggaactgcgg ctcgaggacg taggccgaga ggaggacgac gaggccttcg cggatgtcct
     5761 ccgccgtcac gctgacgccc ttcgggagta gctcgtgcgt ctcgacgtag ttgcgcacgg
     5821 ccttcgtgac gcccgagcgc agcccgttct cgtgcgtgcc cccggacggc gtggagacgc
     5881 cgttcacgaa cgagcggacg tgctcgtccg tcgcctcggt ccagacgagc gcgagctcga
     5941 ggcgggggtc ctccgggcgt tcgaggacga acgcgcccgc gtgcgtcgcg gcctttcccc
     6001 gttgggcgac gacgtgcatc agccagtccg cgagaccgcc ctcgtgcgag agttcttccc
     6061 gcacggccgg cttcttcgtc tcatccgtga acacgacgcg cagcccgcgg tggaggtacg
     6121 tcttggcctc gaggcggtcg cggatctccg ccgcgtcgaa cgcgagcttg ccgaagatct
     6181 cgacgtcggg ccggaagcgc acggtcgtcc ccgagccgcg caccttctcg ccgcccgaga
     6241 tcttcgcgac cttcgacgtc gcctttccct tcgcgtacgt ctgctgccac gtgtggccgt
     6301 cgcgcttgac cgtgacggtc atctcggacg agagcgcgca gacgacggac gagccgacgc
     6361 cgtgcaggcc gcccgagtgc aggtagtttc cctgctcgaa ctttccgccc gcgtggagcg
     6421 tcgtgaggat gagctcgagg gcgctcttct tgtacttcgg gtggacgtcg accgggatgc
     6481 cgcgcccgtt gtccgtgacc tcgatctcct tcccatcggc aaagagcgtc acgtcgaggc
     6541 gggtcgcgaa gccgttgatg acctcgtcga tcgcgttgtc gacgatctcg ttcacgaggt
     6601 ggtggaggcc ccgcgtgtcg acgcctccga tgtacatgcc ggggcgcttg cggaccggat
     6661 cgaggccttc gaggacggtg atgtcgcggg cggtgtaggt ggtggccatg aggcgggcag
     6721 agggtgcggc gaaacgcgct ccgggtcaag cggctcgccc cggttatcgt tccaccccgg
     6781 tgtccgcctt cctcgcgcgc gcgttcgact tcgttcttct cgtcctcgac cggacgggtg
     6841 cgggagcgaa gatcgcgacg ctgccgttct tccccgcagc gatgccgctc gcctcgcgcg
     6901 ccgcgtgggc gctcgcactc ctcggcgctg ccgttctcct cctctactcg tcgctcgcgg
     6961 ccgggcccga ccgccgcgtg gcggcgttcc gcgtcctgac gcttctcggc ggctgtctct
     7021 tcccgctcct cgcggcgagg ctcgtgcccg cggggttcgc gcgtccgttc ggcggcgccg
     7081 tcctcacggt gacgttcacg gccggcctcg ttcaccgcgc tctcgcgggc ctgcggaggg
     7141 agagcgccgc ttcgcgcttc ctcgaaggag cacgctggct gctagaggtc gccgggatcc
     7201 tcctcttcgt cgccgcggct ctcgtcttct tcggcgaggg acgcgtcctc gggcctgctg
     7261 cgctgtgggg gctgctctgc ctgcgcctct cgatcgccga cctcctcgac ccgtcgcgcc
     7321 tcgcgaagga gacgggcctc acgtcgagcg ccgcgaagga cctcaaggcc gcccgccgcg
     7381 gccggacccc ggccgcgcac cgcggcgccc gattcctgtc gggtgtcttc aagctcggac
     7441 tcgccgtcgc ctgggtcttc ctgccgctcc tcgcggccgt cgcgcccggc gagatcgcgc
     7501 gcggcgagtg gccgcccgcg gcgcgtgttc tcctcgccta tcccgccgcg gcgctcttcc
     7561 tgacggggat cctccttctt ctcggcgcga tgaagcgcct cccgtcgagc ccgttcgagg
     7621 ccgcgcgcgg tgcgatcgtc ggcgccggga cgctgctctg gctctgggtc gtgttcgacc
     7681 gggcctcctt cggcgaagcc gtacagatcc tgcccgggct ctatgtcgcc gagacgctcg
     7741 cgggtttcct cttcggagcg gcggcgcgcg ggcgctgatc gttccgacgt aacatctccg
     7801 gatgcccgac acggtctctg gtcgcgaagc tccggtcctt gcccgccgca tcggcttctg
     7861 gagcgccgtc gcggtcgtca tcggctcgac gatcggctcc gggatcttcc gctctccggc
     7921 cggcatcgcg gacaagttgc ccggcccgct ccccatgctc gccgtgtggg ccgcgggcgg
     7981 cgtcttcgcg ctctgcggcg cgctcaccct cgccgaggtc gcgagcgcgc tgccccagac
     8041 gggcggcatg tacgtcttct gccgcgacgg ctggggacgc ctcgccgggt tcctcttcgg
     8101 atggggccag ttctccatga tccgcgcggc gtcgctcggc gccatcgcga tcacgttctc
     8161 ggagtacttc ttccgcgtga cgggccagaa cccgcaggcg ccggacaagg tcatggccgt
     8221 gcgctggttc gcggcgttcg cgatcgcgct cacggcgggc ttcaacatct tcggcgtccg
     8281 cttcgcgtcg aacgtgagca acgtgacggt gatcgcgaag tacggcggcc tcgtcttcat
     8341 cgtcctcgtc gcgctcttcg tcggcctccc gaagacgggc ggcaacttct cgcccgccgt
     8401 gcccgccggc agcttccacg tcgccgcgtt cggtctcgcg ctcgtctcga cgctctgggc
     8461 gttcgacggc tgggccgacc tcgcctacaa cgccggcgag gtgaaggacc cgcagaagaa
     8521 cctcccgcgc gcgctcatcg gcgggacgct tctcgtcgtc gcgatctacc tcgccgcgaa
     8581 cgtcgcgtac ctcgcggtcc tcccgatcga gaagatccgc gtctcgaagc tcgtcgcggc
     8641 cgacgtcgcg caggtcgtcc tcggaacggc cggcgccgcg ttcgtgtcca tcaccgtcat
     8701 ggtctcgacg ttcggaaccc tgaacacggt cctcttcacg agcccgcgcg tcttcttcgc
     8761 gatggcggcc gaccgcctct tcttcgcccc cgtcgcgtcc gtccacccgc gcttcggcac
     8821 gccgtggatc tcgatctcga tgacggcggg cctcgggatc gtcttcgtcc tctcgcgcaa
     8881 cttcgagcag ctcgcggacg cgttcgtcac ggcgttcctg ccgttctacg cgctcgcggt
     8941 cgcgtcgatc ttccggctcc gaaagaagcc cggttacgcc ccgacgttca aagtaccgct
     9001 ctatcccgtg cttcccgtcg tcttcctcgc gtccgtgctc tacctcctcg gcaacgcgat
     9061 cgtccaggag agcagccgca tgagcacgct cgccgtcctc ggcgtcgtgg cggcggggat
     9121 tccggtctac tacctcacgg tcggccggaa gacggcctag cccgccagcc gctcccgcac
     9181 ggcgctctcg atctcgtcgc cgtgcgcgac gaggccgtgg tcgccgcccg cgacgcggtg
     9241 gaagcgcgag cccttcacga gccggccgga cgcctcccag cgctcccagg catcggcgac
     9301 gagcgcgtac ggcacggtct cgtcgtccgt ccccatgacg accgagaccg gaaccggcgg
     9361 cggggcgtcg tcgacgcgca cgagcgccat ctcctcgaag aagcgccggt ggatcggcag
     9421 ctgccgctcc tcgccgtggt ggaagaggtc gaacgtgtcg ctctcgggca gtttctcctt
     9481 ccacctctcg tgaaagccga gtgccggagc gacgaggacg agagggggag gaggagagag
     9541 aagggggaaa gattctgcga gtgaaagagc gaccaaggct cccagcgaac ttccgacaat
     9601 gacgcgcggc cgtgcgcggt cgcacgccgt ccgcgccgca cggaccattt ccgagaaatc
     9661 cagctttcga aagcttggaa cgttcaggtc cggcgccacg atctcgatgc cgtccgggcc
     9721 gaggcgatct tcgagcacgg cccgcttgcg gccaagggga gacgacgcga ggccgtggag
     9781 gtagaggacg gccgacgccg atgccacggt ccgactctag cagcgccgcg cgtcgccccg
     9841 gaccctcgga tttcgacgcg ttgttagaag attctggccc cgtccgcccg gcccgatcca
     9901 atggtttgga gcccccgaag ttgtcgagaa tcacgcgcta gaatcgtggt aagactcgat
     9961 ctgcacgctt acctttaatg aacggacctt ttatttggag gaggtttttg aaatgcggtt
    10021 gatctcgcga atctgtctcg tgctcctcgt caccgtcctc gcgacggcgg cgtgggccgg
    10081 tgagaccggc tcggtgtccg gcgtcgtgaa agacggcgga ggcctcgccg ttcccggcgc
    10141 taccgtgaag atcacgggcg ctcaggcgcc ccgtacgacc gtcacgaacg cgagcggcgc
    10201 cttcaagttc gacgtcctgc tgcccggcga ctacgtcgtc acggcggacc tcaagggcct
    10261 cggctcggcg agccagaaag tccgggccta cgtcgacaac gacgcgcagc tcacgctgat
    10321 cctcgtctcg tccgcgaagg ccgaggtcgt cgtgaccggc gcggccgccg agatcgacaa
    10381 gacgtcgtcc gagagtaact tcaactacac cgacaacacg ctcaaggacc tcccgctttc
    10441 gcggacgtac cagggcgtca tcaagatcat tccgggcgcc cccaccgaca cgagcggcca
    10501 gggcttcgtg tccatttccg gcggcacgcg ccaggacaac aagtacctcc tcgacggcgt
    10561 caacgtcacg aacccgggct acgggtggct tggcgtcgat acgaaccagc tcgacatcgc
    10621 ggacttcaac gtcaagaagg ccggcatctc ggccgagttc ggccgcacgt cgggcgcgat
    10681 gatcaacgcc gtcacgaagt ccggctcgaa ccagatcgcc ggcgccgtcc aggtgaacct
    10741 gtcccccagc tccttccagg cgaaccggtc gttcgacgtc cagtccgaca cggacctcta
    10801 caacggccag gcgaacttcg ggttcccgat catcaaggac acgctcttcg gttacgtgtc
    10861 cggtgcgtac tacgacgcga agacgtccgg ccagtcggcc accgtcggcg gcgtcacgac
    10921 gacgcagccc gactcgaagt cgcacaacgg cgactacttc ggaaagctga cgtacttcgc
    10981 cggccagtcc ctcctcgtga acgcgggctt ccgcgcgctc ccggcgaagt ccaccgacca
    11041 gtttggctcg atctacgacc tgccgagcgc cgcgcagggc gcggacacga cgaactacgt
    11101 cggcaacctc tcggtcgact ggttcgcgtc caagaacacg gtcgtcgagg cgaagtacgt
    11161 ctacctcgag cagaatgaca cctcggcggc gcagacggtg ctcgcgccgt tccagcccgc
    11221 ggtcatcaac cccaagaacc tcggtgccta cggcgcctac gccgacccgg cccgcaatgg
    11281 cggcaacgtc ggcgtgcccg agttcgccga gaccggcgag aagtacaagc gcagcgagat
    11341 caaggccaca ctgagccagt acttcgacgg cggctccatg cagcatgcgt tcaaggtcgg
    11401 cggcggctac gaagacgccc agatggacac cgtccgcgcg acgaacggct ggggcatctg
    11461 ggccaccggc cagacgtgtc ccgcgtcggt ctgcggcacg tccgtgtccg gcacggtccg
    11521 cgcccggtac tacacgtctc agcccgtcca gtactcgctg gctcggacgt actccgcgtt
    11581 catccaggac acgatcacga tgaagaacct gaccgtctac ctgggcgtcc tcgtgaacaa
    11641 ggacgacttc gcccagctgt gcaagacggg caacgtctgc ggctcgtcca cgaccgcgcc
    11701 gaacacgtcc gacgtgcgtt acaacttcat gacgttcgac tggagccagg aaatccagcc
    11761 gcgcctcgga atcacgtgga acacgaacct cctcccgagc gacaagttct acgcgacgta
    11821 cggcgagtac gccggcgtgg accagaagtc gacggcgcgt tccttcgccc cgtaccgcat
    11881 ccgtcaggac caggcctact tcaacgggac gaccggcgtc ttcctcggct cgcagtaccg
    11941 cggctcgtcg ggcggcaagg tcatcccgcc cgacctcaag cctcccaagt acgaggaatt
    12001 ctccgtcggg tactccgccg ccctcaccaa ggacatcagc gtcgacgcgt actaccagta
    12061 ccggaacctc aagaacgcgt tcgaggacgc gccgatcgac gtcaacaact acttcggctc
    12121 cttccaggcg aagaacttct tcaacgcccg ccgcacgtac gacgccctca cgatcgacat
    12181 ccagaagcgg tattcgaact cctggtacgc ggacatgaac gtcacgttca gcaggctcta
    12241 cggcaactgg gacgaggact acggcatcgg cctcttcaac acgtcctcgt tcctcgagga
    12301 cgagccgggc tggaacagcg ccgacccgaa ccgctacggc cacctcggac aggaccgccc
    12361 gatcatcttc aagctgatgg gctcgtacga cctgccgatg ggcttcgtcg tcggagggtt
    12421 cctccgcgtc cagagcggca cgccgtggga ggcccgtgga ggcacgccgt cgacgacgag
    12481 cggccgttac ctcgagcccg ccggcacgaa ccggctcccg acgtggacga ccgtcgacct
    12541 gctcctcgcg tacaacctca agctcggcgg caacatggcc gtccggctcg aggcccgcgt
    12601 cgcgaacgtc ttcgacacgc agacggtcct cagcgtcaac tcgatcaagt acaacgacgg
    12661 gtacgtggac ggcaacccgg ccggcaacct cggctggcag cagacgaacc agccgaacgc
    12721 gaacttcggc aacgcgacga gctggtcctc gccgcggcgt ttcacgctca cggctcgcct
    12781 cgacttctga tctgaatccg ggggaacccc gccgctgaag cggctcgaaa gagggttccc
    12841 ccgaaccccc ttctctctct caacgtgacg gcccggcgga aacgccgggc cgtttcattt
    12901 ggcgccgtcg agcgccttca ggaacgccgc ggccttctcg tagtccggct tcaggacgag
    12961 cgcttcgcgc gcgaggccgc gggcccggtc cgtctccccc gccatcgcgc acgcgacggc
    13021 caggttgagg cgcgccgccg cgttcgcggc gtccagccgc acggcctcct cgaagtcggc
    13081 gcgaccgccc gccacgtcgc cgcctttcat tcgcgagaga ccgcgctgct cgagaagggc
    13141 cgagagtgcg acggcggcct ccggcttcgg cgtagacgac gcgagggagc gcgcgaaggc
    13201 cgcggccgcg gcctcgggct ttccggcggc cgcaaaggag cggcccgcgc gcagccagac
    13261 gaggccgggg tccgggtgcg cctgcgccgc gctctcggcg tgcgccatcg ccccggcttc
    13321 tcccttctcg atctcccaga ggacgaggtg catctcctcg tccggcgcgc cgtcccagag
    13381 gcccgtcggc cagatcgaga cgatcgcgag gactgtggcc gccgccgcgg cgacggcgag
    13441 acgggcgccc ttcgcgcgcc acgcctctcc ggcctgtgtc accgcggcgc cggcgagcgg
    13501 agaaagtgcc acgagcaagg gaaggcggta ccgcgtcgcg acgaagaacg ccgcgacggc
    13561 gaagacgtac gccgggatga acgtggccca gacgagcagc gccggccgcc tctcgctcgc
    13621 ggcgcccgcg gccccgagga ggccgaagac gagacccgcg cccgcgagcg gcacgaggag
    13681 gccgggaccg acgaacagga gcttcagcgc aaggctcttc tcgcggtacc acgggaagct
    13741 gaagttgagc ggggcctcgt cgccggagag cgtgaaccgg atctttcgca gaaagagaga
    13801 ggcggcggag ccggggttcg cgcgaatcca gcgaaatgcc tcgcgcgcga agtgcgagga
    13861 gacttccccg gtcgtcagtg cgcggccctc ggccgcctcc gcgacgcgct tcgtgtcggc
    13921 cgcctggccg ccgatgtcgg gcgtgatccc gggcagctga cggtagacgc cgtccgcgcc
    13981 cggcccgttc ccgacgagga agttcagccc gccgtgcgag gagacgagga cgaattcacg
    14041 ggagacgaag agatttctga gagtgaaggg agcgatcgtc acggccgcac ccgcgacgaa
    14101 ggccgaagcg atccacaccc ttcgaagagg attttccttg aaggtgaaga gcgcaagggc
    14161 cacggcgacc gctgcgacgc agagcagcgc attcgggcgg ttgagggaga gaaaaccgcc
    14221 ggcggccccc gcggccacga tctctcccaa gcgcacacgg gtccccgtcc gcgcctctcc
    14281 cctcttcacc ttcaaggaaa tcttctctct ctcttcctct tcctcttccg aacgaaccgc
    14341 ccttgccagc aggtacagga acagcgcggt gagaaacggg tcgatcgccg cctgcaggat
    14401 caagacctcg tggaacgtca cgacgcccgc gagggcgtaa aggacccccg ccgcgagcgc
    14461 ggcgcgctcg ccgaagagcc ggcgcgccgt gaggaacacg agggcgacgg ccgcggtgcc
    14521 gagcaggatc tggacgacgc gggcgaccgg aaacgagggc cccgtcgccg cgaggaaata
    14581 cgcgtagagc gggctcacga agtacggcac ggcatcgccg cgcagcagca gatctccgcc
    14641 cgcgatgcgg tgcgcgagcc gggcgtacac gccgctgtcg atctcgccgt agggctggag
    14701 caacgggtga ctccccagct gcgcgagcac cgccgtcttc gcggcaagcg tcgcggcgag
    14761 cagcaaaaac aggcgtttca tcagggagga ggttacctcc cctgccttgc taagctggcg
    14821 gcatgtccgc gttctggatc ggcgtcgacg ggggaggcac gcgcacgcgc gccgtcgtgg
    14881 cgggcgagga tctcgtcccc atggggcgcg gcgcatccgg gcccgcgaac gccgcgacgc
    14941 gcccgcttcc gctcgtcgtc gagacgatcc tcgaggccgc cggcgacgcc gcgacctcgg
    15001 ccgtggtctc catccggcgc gccgagcgcg tcggcgtggg cctcgcggga gtggagtccg
    15061 cgggcctcgt ccagccgctg accgccgccc tcgaggagca cttcggggcg ggccgcgtct
    15121 tcgtgacgac ggacgcgcgc atcgcgctcg cgggcgccgt tgcgggcgac cccgacgggc
    15181 cgggcgtggt cctgatcgcg gggacgggcg ccatcgcctt cggccgcggc acggacggcg
    15241 cc
//
LOCUS       DAOMFY010000069        15123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1207_length_15123_cov_8.024489, whole genome shotgun sequence.
ACCESSION   DAOMFY010000069 DAOMFY010000000
VERSION     DAOMFY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15123
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1207_length_15123_cov_8.024489"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLB01_07675"
     CDS             <1..778
                     /locus_tag="PLB01_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQQ77220.1"
                     /translation="DKPRARHIVIVGAGLNGTNVARVLQETGIPHVLVDIDPERIADA
                     RREGLDALRADATGPEGLGAAGVARALGVVVTIPDPDACRRVVRLSRMNAPNVRILVR
                     TRYVKEVETLRRLGADEVIPEEFETSIELVARVLRLLHVPGNVVATQIRLLRDEAYRR
                     LRDPQSKAASGRRLSALVAAGTSELFLVLPDTAADGRLLSELDLAAAHVAVPAVLRDG
                     VPHAPPPEDFKLAAGDTLVLVGAHEDLARVLARLEAPAAG"
     gene            803..2728
                     /locus_tag="PLB01_07680"
     CDS             803..2728
                     /locus_tag="PLB01_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HQQ77221.1"
                     /translation="MKKPFLLLAALALAAPAAAQDAGRFEFGIFGGVSIGSRISLTPG
                     SDIRIGDAGAFGLRGAYDFNRHFALEASWSHARPTATTRHPTTGATLGETAVNLDTYE
                     ITALYNYGRGRVRGYFGLGAGVMSIDPVLAASAGPTARFTASLSAGGKWFVTPSVALR
                     LGGAYRFRTTDDRISTVLCGDGCKTYTTSFYSNAELTAGVSFRFGGDPFADVLSSADR
                     ESESQKRFWAAAGSVAFFDVVPWAFNRYVTKAEFAYISTDTFRANVTAGFGYDRDSFK
                     TDQSSHPFHGSLFFNSARSNGYGFWESGIAATLGSFVWELGLEKEPPAINDLVNTALG
                     GMSRGEVFHRLSTMLRDNTASGSSRFWRELGAGVMDPAGFLTRLTRGELSGSRPNPED
                     RMPGAFRLDGDLGYRHVGGDASNPDQGLLSLSLLYGNPFAGAMNKPFDSFSLGMDISV
                     PATPLMTRFELRGVLAGRELTDASSSSRHILGVFMEYEYVDNQALVLGAQAFSGGLLS
                     RYALKNGFSLATSATGVAYPLAGVGTTDVLNPISGRSFDYGPGGGFRATARLERKGED
                     VLSAAYAIGWFHTLDGSSKNNMLQFFRVTGRWPLGRKLGVGAGYSWYSRKTTYVNFFE
                     GTRTQPEWRAFVSWKLL"
     regulatory      2789..2882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2882..5020
                     /locus_tag="PLB01_07685"
     CDS             2882..5020
                     /locus_tag="PLB01_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ77222.1"
                     /translation="MAPRFFRTLSALAFVCAGRALAEVAAATATPTPTPTPVPAPTRS
                     ENVTVQAIRGEDEAPVTKTDLDRPALVEKAWGQELPVLLQDTPSVTQYSETGSGQGYA
                     YFTLRGLSQTRVNMTFDGVPLNEPEDSAVYTADFGNLSLNLASVQVQRGVGTSTFGAA
                     AFAGSVNLESLDATREKGLSVSLGGGSFGTAQASAIWNAGTFGGGWRLSLRPTWQTTD
                     GFREHSGVQQDSLFFTAVRDFETSSLRISGFVGEERIQQAFYAVEPEILEKNLAYNPM
                     SPDERDHFHEYLATAQWTLALGDDASLSLQPYAVGAGGWYRLYADAERTNLQEYGLSW
                     QFGGGIATYTRKLGAFRLTLGANAYGYKSTHTQDDGDGTRNYTNHGTKSELSGFVKLL
                     GDLGTWHPFLDAQVRHARLGYEGDVGETSQGWTFFNPKAGVRVDLSPRASAYVSVGRT
                     EREPGRSDLLYGEDNASVLPDLAAVQPESVWDFELGAEWKTKSLLFKVNLYDMEFTNE
                     IAATGELSAIGQPLRRNVDRSFRRGIEIEAAWQALQALRISASMNASRNRISSWTQYY
                     DVYDANGDYAGATSRTFQNVAPVATPPFQATLAAETTALKGVTVGAAGRYVAASWLDN
                     TNAEGLEAPRTFQLDATFVVDLSRWIPAGKPRLKVQVNNVLDDRRLWPRGYSYLYASR
                     DAAGAETLGGIPYLYPLATRSAYVSLDVGF"
     gene            5025..5552
                     /locus_tag="PLB01_07690"
     CDS             5025..5552
                     /locus_tag="PLB01_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ77223.1"
                     /translation="MRRVVLTGGECTGKTTLARALAARWGTAWSAEAAREVALARSGV
                     LGPEDVSVIARTHVRLADEAARAASEAGRPLVFFDQDLVSTSVYARHYYGDCPLWIER
                     LSAERQGDLYLLCRPDLPWESDGVRDRPAAREGIHALFAETLAAANARVVDVQGLGPA
                     REALAASAVTALLGT"
     gene            5590..6159
                     /locus_tag="PLB01_07695"
     CDS             5590..6159
                     /locus_tag="PLB01_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HQQ77224.1"
                     /translation="MASALAVVLASALALPGGEPAGREGVPGVRNFAVVSPVLSRGAQ
                     PTREGFEALRERGIRTIVDLRWLHGNEKALAGLGLNSYRLRAKQWHPETEDVVRAMKV
                     ILTPEYQPVFVHCQAGRDRTGLIVAVYRILVDGWSVEDAIAERKQFGAADFWEDSKTY
                     LRRLESPEARKRLLAAIEAAPVPPVVRVP"
     gene            complement(6165..7109)
                     /locus_tag="PLB01_07700"
     CDS             complement(6165..7109)
                     /locus_tag="PLB01_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HQQ77225.1"
                     /translation="MARKPDSGKERSARTDLASFIKKARKFAGPYRVDKGKKFRLKDV
                     DPGDTAGLGSEAKPQAKAALQNGIQALAELQDMLYAQDRWAVLLIFQAMDAAGKDGAI
                     KHVMSGVNPQGCQVASFKAPSAEELDHDFLWRCNRHLPERGRIGIFNRSYYEDVLVVK
                     VHPEFLAGAKLPPRLVKKDVWENRYRDIRNYERYLANNGVVVRKFFLHVSRKEQKRRF
                     LERIENPEKNWKFSPSDAKERARWKDYMRAYEDMIRETSTEESPWYVVPADNKWFTRV
                     VVAAAVIEALDSLGLHYPEVGPEKLRELAAVKKALLAE"
     gene            7199..8215
                     /gene="gnd"
                     /locus_tag="PLB01_07705"
     CDS             7199..8215
                     /gene="gnd"
                     /locus_tag="PLB01_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HQQ77226.1"
                     /translation="MQLGMIGLGRMGANMVRRLIGGGHDCVVFDVSPKAVADLARERA
                     HGATSLADFVGKLAKPRAVWLMVPAAVVDRTIADLLPLLENGDVLIDGGNSYYVDDIR
                     RAKELAARGIHYVDVGTSGGVWGLERGYCMMIGGETDVVERLDPVFKRLAPGRGDAPR
                     TPGREKARGTAEEGYLHCGPNGAGHFVKMVHNGIEYGLMAAYAEGFNILRNANVGKKG
                     RAVDAETTPLRHPEHYQYDMNLADIAEVWRRGSVVASWLLDLTAIALLESPDLAGFSG
                     RVSDSGEGRWTIQAAIEEGTPAEVLTAALYERFSSRGEADFQNKILSAMRFQFGGHHE
                     KPSE"
     gene            8246..8713
                     /locus_tag="PLB01_07710"
     CDS             8246..8713
                     /locus_tag="PLB01_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77227.1"
                     /translation="MKTPMATFVLAAAAAAAFSLSALAQEPAPTPAAAPAAAASAPAP
                     AVNIPPKTYFEAFEVTISGKAKAAGAVVLVFQTIGGEPKAATVNVLAKTGENDIARDA
                     AKELTVAAGSAFKVKTKSGNVIVIQKASKNAPNLNISLGMQTVPGVAVSFGEY"
     gene            complement(8723..9886)
                     /locus_tag="PLB01_07715"
     CDS             complement(8723..9886)
                     /locus_tag="PLB01_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ77228.1"
                     /translation="MPPAGEPLVSVVMPCLNEAETVARCVAEARDSLAASGLAGEVLV
                     ADNGSTDGSQALAEAAGARVVNVPGKGYGAALAGGFAAARGTYVVMGDADLSYDFGAV
                     PDFVKALAAGADLVMGSRFAGRIEEGAMPPLHRWLGNPVLSGLGRLFFRTPVSDFHCG
                     LRGFRRAALETLDLRTTGMEYASEMVVKAALFGLVIREIPVTLRRDGRSRPPHLRTWR
                     DGWRHLRFLLLYSPRWLFLVPGLLLALGGAALILWLLPGPRVAFGATWDVHSILGGAA
                     MILVGAQAVFFGACAKIFGITEGLLPKDPFLESLFDRWITLETGLAVSVLALLAGAGL
                     VGHVVSAWSAAGFHDLAYGTTMRWMIPGVLFLVLGAEGVFGSFLLSLLGVRRR"
     gene            9947..10756
                     /locus_tag="PLB01_07720"
     CDS             9947..10756
                     /locus_tag="PLB01_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HQQ77229.1"
                     /translation="MTTTFRVSLVDYVNAWPLTWGFLRGAVEGADRITDVPSACADRL
                     ARGEVDAGLVPSIEAARIPGVRIVRGVGIASKERVRSVILASRRPLEQARTVALDLSS
                     RSSAAMARILFKDLLGTAPEFHTAAPDLAEMLAHHDAALLLGDPALKADLTGLHVLDL
                     AEGWRQLTGLPFVFAVWAVRPSIPPEPFLWSREYAKTHLDEIVTAAVARTGLEAGELR
                     AYVDGNLHHDLSSDDEKGLREFYRRAHAHGLLPSPDAPPFVGVPLVPLRES"
     gene            10758..11945
                     /gene="mqnC"
                     /locus_tag="PLB01_07725"
     CDS             10758..11945
                     /gene="mqnC"
                     /locus_tag="PLB01_07725"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HQQ77230.1"
                     /translation="MSAALDVPAVLPNAAAPGLGGSPRVREVLARAVAGGRLSWEDGL
                     ALFREATLLEAGGAADAVRRRLHPPGRVTYQVDRNINYTNVCIYRCAFCAFYRPAGDP
                     EGYVLSFDEIGKKVEETIALGGTGILLQGGVHAELSFSFYEDLLSFLRASYPRVHRHT
                     FSAPEIHFLAKKEKTTPDDVLARLQKAGMQSLPGGGAEILEDEVRKRIWSLTKAPTEK
                     WAEVHRAAHRLGMPTTATMMFGVGETHEQRLHHLDVVRRAQDESLAEGGESFTAFIPW
                     TFQEENTALDGRVETAGGADYLRTLAIARLYLDNVPHVQGSWLTQGAKIGSVAAAFGA
                     DDLGSIMIEENVVAAAGSHNRMAGEGAMRALIEGSGFTPVKRGSVYDRCRDACCRDLP
                     ALA"
     gene            complement(11965..12666)
                     /locus_tag="PLB01_07730"
     CDS             complement(11965..12666)
                     /locus_tag="PLB01_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006104128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQQ77231.1"
                     /translation="MITVRPARERGHANHGWLDTWHSFSFADYYDPRHMGFRTLRVLN
                     DDTVQPGEGFGTHGHRDMEIVSYVLEGALAHKDSMGNGSTIVPGDVQYMSAGTGVMHS
                     EFNGSDTEPVHFVQIWILPDRAGHKPKYAEKRFEKAEKTNRLRLVVSPDGADGSLAIR
                     QDVRLYASVLEKGAETSLTLPKERHLWVQALRGKVEVNGTSLSAGDGLSASEETAFTF
                     RAPGGEAEFLVFDLA"
     gene            complement(12758..14308)
                     /locus_tag="PLB01_07735"
     CDS             complement(12758..14308)
                     /locus_tag="PLB01_07735"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HQQ77232.1"
                     /translation="MLEDFRNEPFTDFSRAEERSAFEAALNRVDRQKGAEIPVVVGGR
                     RLKTGRLLENTNPSDRSEVLSRHHLADRRIALKAVEAVVKAQKPWAELPARERASVLL
                     RAAARMRAKKHEFSATMVLEIGKSWPEADVETAEAIDFLEFYAREMLRWGGEIPVTPY
                     PGEFPETLYVALGAGAVIPPWNFPNAILTGMTAAALVTGNGVVLKPASDTPLIGWRVF
                     TLLEEAGVPAGALNYLPGPGSEVGDLLVEHAQTRFISFTGSKQVGLTINQKAAAVAKG
                     QRWIKRVVLEMGGKDFIFVDEDADFDFAVSQVVASAFGFQGQKCSACSRAIVHKRLYK
                     RFVDAVVAKTAKLTQGPTRDPKNFMGPVASERQFKTVTDYIRVGRRESKLGTGGTWHG
                     RVGNFVAPTVFYDVKPTARIFQEEIFGPVLGITEAKDFETGIAYANASEYGLTGSYFG
                     RDRHRIAEAKRRLHCGNLYINRKCTGALVGVHPFGGFDMSGTDSKAGGRDYLGLFLQA
                     KSISEKLL"
     gene            14353..14661
                     /locus_tag="PLB01_07740"
     CDS             14353..14661
                     /locus_tag="PLB01_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQQ77233.1"
                     /translation="MTVEELKGKLDAGEAVCVLDVREARELEVARYPFTVLHIPMSQV
                     PARLDEIPKDRTVVCACRSGSRSAHIAAFLKSHGRDAVNLDGGILAWSAHIDPSIPRY
                     "
     gene            14743..>15123
                     /locus_tag="PLB01_07745"
     CDS             14743..>15123
                     /locus_tag="PLB01_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77234.1"
                     /translation="MDKNVAKIRGFVTDLLRKNKALTEREVKAAIGRAFKLNPKTISA
                     GVIRDVRKKLGIDRPGAIAFAKALLDKNPVLEAKKVIEEVGTRFGVRLGAPDVSRLRP
                     VGARRRLGGPRPDRVAMRKTATRGG"
BASE COUNT         2281 a         5254 c         5334 g         2254 t
ORIGIN      
        1 ggacaagccg cgcgcgcgac acatcgtcat cgtgggcgcg gggctcaacg ggacgaacgt
       61 cgcgcgcgtg ctgcaggaga ccggtattcc gcacgttctc gtggacatag accccgagcg
      121 catcgcggac gcgcggcggg aagggctgga cgccctgcgg gccgacgcga cgggtccgga
      181 ggggctgggc gccgcgggcg tcgcgcgcgc gctcggcgtc gtcgtgacga tccccgaccc
      241 cgacgcctgc cggcgggtcg tgcgcctgag ccgcatgaac gcgccgaacg tccgcattct
      301 cgtgaggacg cggtacgtca aggaggtcga gacgctgaga cgcctcgggg ccgacgaagt
      361 catcccggag gagttcgaga cctcgatcga gctcgtcgcg cgcgtcctgc gcctccttca
      421 cgtgccggga aacgtcgtcg cgacgcagat ccgccttctc cgcgacgagg cgtaccgccg
      481 cctgcgcgac cctcagtcga aggcggcatc cgggcggcgt ctctcggcgc tcgtggcggc
      541 ggggacgagc gagctctttc tcgtcctgcc cgataccgcc gcggacggcc ggctgctctc
      601 cgagctggac ctcgccgccg cacacgtcgc ggtgccggcc gtactccgag acggggtccc
      661 gcatgcgccg ccgccggaag acttcaagct cgccgcgggc gacacgctcg tcctcgtcgg
      721 cgctcacgag gacctcgcgc gcgtgctcgc gcgcctcgag gcgcccgcag cgggctgacg
      781 cttctccggc tatattcgca agatgaagaa accgttcctt ctcctggccg cgctcgcgct
      841 ggccgctccg gccgcggcgc aggacgccgg gcgcttcgag ttcggaatct tcggcggcgt
      901 ctcgatcggc agccgaattt ccctgacacc aggctccgac atccggatcg gggacgcagg
      961 cgctttcggg ctccgcggcg cgtacgactt caaccgccat ttcgccctgg aggcgagctg
     1021 gtcgcacgcg cgccccacgg cgacgacgcg ccacccgacg acgggggcga cgctcggcga
     1081 aacggcggtc aacctcgaca cctacgagat caccgcgctc tacaactacg ggcgcggacg
     1141 cgtccgcggg tacttcggcc tcggcgccgg cgtgatgagc atcgaccccg tcctcgcggc
     1201 ctccgcgggc ccgacggcgc gcttcacggc gagcctctcg gccggcggga aatggttcgt
     1261 gacgccgagc gtcgccctgc gcctcggcgg ggcgtaccgc ttccggacca cggacgaccg
     1321 gatcagcacg gtcctctgcg gcgacggctg caagacctat acgacgagct tctattcgaa
     1381 tgccgagctg accgcgggtg tgtcgttccg cttcggcggc gatccgttcg cggacgtgct
     1441 ctcgtccgcg gaccgcgaat cggaatcgca gaagcggttc tgggccgccg cggggagcgt
     1501 cgcgttcttc gacgtcgtgc cgtgggcgtt caaccggtac gtcacgaagg ccgagttcgc
     1561 gtacatctcg acggacacgt tccgcgcgaa cgtcacggcg ggcttcgggt acgaccgtga
     1621 ctccttcaag acggaccagt cctcccaccc gttccacggg agcctcttct tcaactccgc
     1681 gcgctcgaac ggctacggct tctgggagtc ggggatcgcc gccaccctcg gcagcttcgt
     1741 gtgggagctg ggcctcgaga aggagccgcc ggcgatcaac gacctcgtga acacggcgct
     1801 cggcggcatg tcgcgcggcg aggtgttcca ccgcctctcg acgatgctgc gggacaacac
     1861 cgcttccggc tcgagccgct tctggcgcga gctcggcgcg ggcgtgatgg accccgcggg
     1921 gttcctgacg cgcctcacgc gcggcgagct cagcggctcg cggccgaacc ccgaggaccg
     1981 gatgcccggg gctttccggc tggacggaga cctcggctac cgccacgtcg gcggcgacgc
     2041 ctcgaatccc gatcagggcc tcctgagcct ttcgctcctc tacggcaacc cgttcgccgg
     2101 ggcgatgaac aagcccttcg actcgttctc gctcgggatg gacatctcgg tgccggccac
     2161 gccgctcatg acccggttcg agctgcgcgg cgtcctcgct ggacgcgagc tgaccgacgc
     2221 gtcgtcctcc tcgcggcaca tcctcggcgt cttcatggag tacgagtacg tggacaacca
     2281 ggccctcgtc ctcggggccc aggccttcag cggcggcctc ctctcgcgtt acgcgctcaa
     2341 gaacgggttc agcctcgcga cgagcgcgac cggagtcgcc tatccgcttg ccggcgtcgg
     2401 gacgacggac gtcctgaacc cgatctcggg ccggtcgttc gactacgggc cgggcggagg
     2461 tttccgggcc acggcgcggc tggagcggaa gggcgaggac gtcctctcgg ccgcctacgc
     2521 catcggctgg ttccacacgc tcgacggctc ttcgaagaac aacatgctgc agttcttccg
     2581 cgtcacgggc cgctggccgc tcggccggaa gctcggcgtg ggtgcgggct attcgtggta
     2641 cagccggaag acgacgtacg tgaacttctt cgagggcacg aggacgcagc ccgagtggcg
     2701 cgccttcgtg agctggaagc tgctctgacg gcgccgcggt gatgtccggc ggcgggggtt
     2761 gactccccgg ccgccggggc gcagattggt cgctgcctgg gggtggcccg agagggcctg
     2821 agatcacacc ccggaacctg atccggttcg aaccggcgta gggaaaggca gaaggaacac
     2881 catggcgccc aggtttttcc ggactctttc agctctggcc ttcgtgtgcg cgggccgtgc
     2941 gctcgcggag gtcgcagccg cgacggcaac gcctactcct actcccacgc ccgtcccggc
     3001 gcccacgcgc tccgagaacg tcaccgtgca ggcgatccgc ggcgaagacg aggcgcccgt
     3061 cacgaagacc gacctcgacc ggccggcgct cgtggagaag gcgtgggggc aggagcttcc
     3121 cgtcctcctc caggacacgc cgtccgtcac gcagtactcc gagacgggct cggggcaggg
     3181 ctacgcgtac ttcacgctgc gcggcctctc gcagacgcgc gtgaacatga cgttcgacgg
     3241 cgtgccgctg aacgagcccg aggattccgc cgtctacacg gccgacttcg ggaacctctc
     3301 cctcaacctc gcgagcgtcc aggtccagcg cggagtcggg acgtcgacgt tcggcgccgc
     3361 ggcgttcgcg ggctccgtga acctcgagag cctcgacgcg acgcgcgaga agggcctgtc
     3421 cgtctcgctc ggcggcggct cgttcgggac ggcacaggcg agcgcgatct ggaacgcagg
     3481 gacgttcggg ggaggctggc ggctctcgct gcgcccgacc tggcagacga cggacgggtt
     3541 ccgcgagcac tcgggcgtcc agcaggacag cctcttcttc acggccgtcc gcgacttcga
     3601 gacgtcgagc ctccggatct ccggcttcgt cggagaggag cgcatccagc aggcgttcta
     3661 cgccgtcgag ccggagatcc tcgagaaaaa cctcgcgtac aacccgatgt cgcccgacga
     3721 gcgcgaccac ttccacgaat acctcgcgac ggcgcagtgg acgctcgcgc tcggcgacga
     3781 cgcgagcctc tcgctccagc cctacgccgt gggcgccggc ggctggtaca ggctctacgc
     3841 ggacgcggaa cgcacgaacc tgcaggaata cggcctgtcg tggcagttcg gcggcgggat
     3901 cgcgacgtac acgcggaagc tcggcgcgtt ccgcctgacg ctcggcgcga acgcgtacgg
     3961 ctacaagtcc acgcacacgc aggacgacgg ggacgggacg cgcaactaca cgaaccacgg
     4021 gacgaagagc gagctgagcg gcttcgtgaa gctgctcggt gacctgggga cgtggcaccc
     4081 gttcctcgac gcccaggttc ggcacgcgcg gctcggctac gagggagacg tcggcgagac
     4141 gtcgcagggc tggacgttct tcaacccgaa ggccggcgtc cgcgtggacc tctcgccgcg
     4201 cgcgagcgcg tacgtctccg tgggccgcac ggagcgggag cccggccgca gcgaccttct
     4261 ctacggggaa gacaacgcgt cggtcctccc ggacctcgcc gccgtgcagc ccgagagcgt
     4321 ctgggacttc gagctcggag ccgagtggaa gacgaagagc ctgctcttca aggtaaacct
     4381 ctacgacatg gagttcacga acgagatcgc ggcgacgggg gagctctcgg ccatcggcca
     4441 gcccctgaga cggaacgtgg accggagctt ccgccgaggg atcgagatcg aggcggcgtg
     4501 gcaggcgctt caggccctgc ggatctcggc ctcgatgaac gcgagccgca acaggatctc
     4561 gtcgtggacg cagtactacg acgtctacga cgcgaacggc gattacgccg gcgcgacgag
     4621 ccggacgttc cagaacgtcg cgccggtcgc gacgccgccc ttccaggcga cgctcgcggc
     4681 cgagacgacg gcgctcaagg gcgtgacggt cggcgcggcc ggccggtacg tcgcggcctc
     4741 gtggctcgac aacacgaacg ccgaagggct cgaagccccc cggacgtttc agctggacgc
     4801 gacgttcgtc gtcgaccttt cgcgctggat cccggcggga aagccccggc tcaaggtgca
     4861 ggtcaacaac gtcctcgacg accggaggct ctggccacgc ggctactcgt atctctacgc
     4921 gagccgcgac gccgcgggcg ccgagaccct cgggggcatc ccgtacctct atccgctcgc
     4981 gacgcgcagc gcgtacgtgt cgctggacgt gggattctga agagatgcgc cgcgtcgtcc
     5041 tgaccggggg cgagtgcacg ggcaagacga cgctcgcgcg ggcgctcgcc gcgcgatggg
     5101 ggacggcctg gtccgccgag gccgcgcgcg aggtcgcgct cgcccgctcg ggcgtcctcg
     5161 gccccgagga cgtgtccgtg atcgcgcgca cgcacgtccg gctcgcggac gaggcggcgc
     5221 gcgcggcgtc ggaagccggc cgcccgttgg tcttcttcga ccaggacctc gtgtcgacct
     5281 cggtgtacgc gcgccactac tacggggact gcccgctctg gatcgagcgc ctctccgcgg
     5341 agcggcaggg cgacctttac ctcctctgcc gacccgacct tccgtgggag tccgacggcg
     5401 tccgcgaccg ccccgcggcg cgggagggga tccacgcgct cttcgcggag acgctcgccg
     5461 ccgcgaacgc gcgggtcgtg gacgttcagg gactcgggcc cgcccgcgag gccctcgccg
     5521 cgagcgccgt gacggccctc ctcgggacct gacggaggcg gccgtccggc gggtgtatac
     5581 cttggcgtca tggcgtccgc tctcgccgtc gttctcgcct ccgcgctcgc gctccccggc
     5641 ggcgagcccg ccgggcgcga gggggttccg ggcgtgagga acttcgcggt cgtgtcgccg
     5701 gtcctgtcgc gcggcgcgca gcccacgcgc gagggcttcg aggcgctgcg cgagcgcggg
     5761 atccggacga tcgtcgacct gcgctggctg cacggcaacg agaaggcgct cgccgggctc
     5821 gggctgaact cctaccggct gcgggccaag caatggcatc cggagacgga ggacgtcgtg
     5881 cgcgcgatga aggtgatcct cacgccggag taccagcccg tcttcgtcca ttgccaggcc
     5941 ggaagggacc ggacgggtct catcgtcgcg gtctaccgga tcctcgtcga cggatggagc
     6001 gtggaggacg cgatcgccga gcggaagcag ttcggcgctg cggatttctg ggaggacagc
     6061 aagacgtatc tgcggcggct cgagagcccg gaggcgagaa agaggcttct cgcggccatc
     6121 gaggccgcgc cggtgccccc cgtcgtccgc gtgccgtagg ccgcctattc cgcgaggagc
     6181 gccttcttga cggccgcgag ctcgcgaagc ttctccgggc ccacctccgg gtagtgcagg
     6241 ccgagcgagt ccagcgcctc gatcacggcc gctgcgacga cgacccgcgt gaaccacttg
     6301 ttgtccgccg ggacgacgta ccagggcgac tcctccgtgg aggtctcgcg gatcatgtct
     6361 tcgtaggccc tcatgtagtc cttccagcgg gcgcgctcct tcgcgtccga gggagagaac
     6421 ttccagttct tttcggggtt ctcgatccgc tcgaggaacc gccgtttctg ctccttgcgc
     6481 gacacgtgga ggaagaactt cctcacgacg actccgttgt tcgcgaggta ccgctcgtag
     6541 ttccggatgt cccggtagcg gttctcccac acgtccttct tgacgagccg gggcggcagc
     6601 ttggcgcccg cgaggaactc ggggtgcacc ttcacgacga ggacgtcctc gtagtaggag
     6661 cggttgaaga tgccgatgcg accccgctcg gggagatgcc tgttgcagcg ccagagaaag
     6721 tcgtggtcga gctcctcggc cgacggcgcc ttgaacgacg ccacctggca gccctgcggg
     6781 ttgacgccgg acatcacgtg cttgatggcc ccgtcctttc cggcggcgtc catcgcctgg
     6841 aagatcagga gaacggccca tcggtcctgc gcgtagagca tgtcctgcag ctccgcgagg
     6901 gcctggatgc cgttctggag ggccgccttg gcctgcggct tcgcctccga gccgaggccc
     6961 gcggtgtcgc cgggatcgac gtccttcagc cggaacttct ttcccttgtc gacgcggtag
     7021 ggaccggcga acttccgcgc cttcttgatg aacgacgcga gatccgtgcg ggcggagcgt
     7081 tccttcccgg aatccggttt gcgggccatg ggctctcctt cgggcccgct agcctacgcc
     7141 cgtcgccgcc cgggggtcct tcggagagaa tgagcgccgc gaaggaaagg ggcctgccat
     7201 gcaactcgga atgatcggac tcggacggat gggggcgaac atggtgcgcc ggctgatcgg
     7261 cgggggccac gactgcgtgg tcttcgacgt gtcgccgaag gccgtcgcgg acctcgccag
     7321 ggaacgggcc catggagcaa cctcgctcgc cgacttcgtc ggaaagctcg cgaagccgcg
     7381 ggccgtgtgg ttgatggtcc cggcggccgt cgtggatcgg acgatcgccg acctcctgcc
     7441 gctcctcgag aacggggacg tcctgatcga cggcgggaat tcgtactacg tcgacgacat
     7501 ccgccgggcg aaggagctcg ccgcccgggg gatccactac gtggacgtcg gaacgagcgg
     7561 cggcgtctgg ggcctcgagc gcggctactg catgatgatt ggcggcgaaa cggacgtcgt
     7621 ggagaggctg gatcccgtct tcaagcggct cgcgccgggc cggggagacg cgccgaggac
     7681 gccggggcgc gagaaggcgc gaggcacggc ggaggagggc tacctccact gcggaccgaa
     7741 cggggccggc cacttcgtca agatggtcca caacgggatc gagtacggcc tcatggcggc
     7801 gtacgccgaa gggttcaaca tcctccggaa cgcgaacgtc ggaaagaagg gccgcgcggt
     7861 cgacgccgag acgacgcctc tccgccaccc cgagcattac cagtacgaca tgaacctcgc
     7921 ggacatcgcc gaagtctggc gccgcgggag cgtcgtggct tcgtggctcc tcgacctgac
     7981 ggccatcgct ctcctggagt cgccggacct ggcgggcttt tcgggccggg tctcggactc
     8041 gggcgaaggg cgctggacga tccaggccgc gatcgaggaa ggcaccccgg ccgaggtcct
     8101 caccgccgcc ctttacgagc gcttcagctc gcgcggcgag gcggatttcc agaacaagat
     8161 tctttccgcg atgcgcttcc agtttggcgg ccaccacgag aagccctcgg agtgaaaatg
     8221 cgatagaact ccgcgaggag gacctgtgaa gacacccatg gctacgttcg ttctcgcggc
     8281 ggccgcggca gccgcgttct ctttgtccgc gctcgcgcag gaaccggccc ccacgccggc
     8341 ggccgcgccc gccgccgccg cgtcggctcc ggcgcccgcc gtcaacatcc cgccgaagac
     8401 ctacttcgag gcgttcgagg tcacgatttc cgggaaggcg aaggccgcgg gagccgtcgt
     8461 cctcgtcttc cagaccatcg gcggtgagcc gaaggcggcg acggtgaacg tgctcgccaa
     8521 gacgggcgag aacgacatcg cccgggacgc cgcgaaggag ctgaccgtcg ccgcggggag
     8581 cgcgttcaag gtcaagacga agagcgggaa cgtcatcgtg atccagaagg cgtcgaagaa
     8641 cgccccgaat ctcaacatct cgctcggcat gcagaccgtt ccgggcgtcg ccgtctcgtt
     8701 cggcgagtac tgaacgtcga ccctagcgcc ggcgcacgcc gaggagcgag agcaggaacg
     8761 aaccgaacac gccctcggcg ccgagcacga ggaagaggac cccggggatc atccagcgca
     8821 ttgtcgtgcc gtaggcgagg tcgtgaaagc ccgcggcgct ccacgccgac acgacgtggc
     8881 cgacgaggcc cgcgccggcg aggagcgcga gaaccgagac ggcgaggccc gtctcgagcg
     8941 tgatccaccg gtcgaacagg gattcgagga aggggtcctt cgggagaagt ccttcggtga
     9001 tgccgaagat cttcgcgcag gcgccgaaga acacggcctg ggcgccgacg aggatcatcg
     9061 ccgcgccgcc gaggatcgag tggacgtccc acgtcgcgcc gaacgcgacc cgcggccccg
     9121 gcagcagcca caggatcagc gccgcgccgc cgagcgccag gagcaggccg ggcacgagaa
     9181 agagccagcg cggcgagtag aggagcagga aacgcaggtg gcgccagccg tcccgccagg
     9241 tccgcaggtg cgggggcctc gaccggccgt ccctgcggag cgtgacggga atctcccgga
     9301 tcacgaggcc gaagagggcc gccttgacga ccatctcgct cgcgtactcc atcccggtcg
     9361 tccggaggtc gagggtctcg agcgcggcgc ggcggaagcc gcgcaggccg cagtgaaagt
     9421 ccgagaccgg cgtccggaag aagaggcgcc cgaggccgga gaggacgggg ttgccgagcc
     9481 agcggtggag cggcggcatc gcgccctcct cgatgcgtcc ggcgaaacgc gagcccatca
     9541 cgaggtccgc gcccgccgcg agggccttca cgaagtccgg cacggccccg aagtcgtacg
     9601 agaggtcggc gtcgcccatg acgacgtacg tgccgcgcgc ggcggcgaag ccgcccgcga
     9661 gcgccgcgcc gtagcccttc ccgggaacgt tcacgacgcg cgcgccggcg gcctcggcca
     9721 gcgcctgtga cccgtccgtc gagccgttgt cggcgacgag cacctccccc gcgagcccgc
     9781 tcgccgcgag cgagtcgcgc gcctccgcga cgcaccgcgc caccgtctcg gcctcgttca
     9841 ggcacggcat caccacggaa acgaggggct ctccggcggg cggcatggcc gattgtcgcc
     9901 gagtccgccc cgggtctggc ggcgtagtgt atttttgaag agggccatga cgaccacgtt
     9961 ccgggtttcc ctcgtcgact acgtcaacgc ctggccgctg acgtggggat tcctgcgggg
    10021 cgccgtcgag ggcgcggacc ggatcacgga cgtcccgtcg gcctgcgcgg accgcctcgc
    10081 gcgcggcgag gtggacgccg gcctcgtccc gtcgatcgaa gcggcgcgca tccccggcgt
    10141 ccgcatcgtc cgcggcgtcg ggatcgcgtc gaaggagcgc gtccggagcg tcatcctcgc
    10201 gagccgccgc ccgctcgagc aggcccgcac ggtcgcgctc gacctctcgt cgcgctcctc
    10261 ggccgcgatg gcgcgtatcc tcttcaagga cctcctcggc acggcgcccg agttccacac
    10321 ggccgcgccg gacctcgccg agatgctcgc gcaccacgac gcggcactcc tcctcgggga
    10381 tcccgcgctg aaggccgacc tcacggggct ccacgtcctc gacctcgcgg agggctggcg
    10441 ccagctgacg ggcctgccgt tcgtcttcgc ggtctgggcg gtgcggccct cgatcccgcc
    10501 cgagcccttc ctctggtcgc gcgagtacgc gaagacccac ctcgacgaga tcgtcacggc
    10561 ggccgtggcg cgcaccgggc tcgaggccgg ggagctccgc gcctacgtcg acggcaacct
    10621 gcaccacgac ctctcgtccg acgacgagaa gggactccgc gagttctacc gccgcgcgca
    10681 tgcgcacggt ctccttccct cgccggacgc gccgccgttc gtgggcgtgc cgctcgtccc
    10741 gctgagggag agctgagttg tccgccgcac tcgacgttcc ggccgtcctt ccgaacgccg
    10801 ccgcgccggg actcggcggc tccccgcgcg ttcgcgaggt cctcgcgcgc gccgtcgcgg
    10861 gcgggcggct ttcgtgggag gacggcctcg cgctcttccg cgaggcgacg ctcctcgagg
    10921 ccggcggcgc ggccgacgcc gtgcgccgcc ggctccaccc accgggccgc gtgacctacc
    10981 aggtcgaccg gaacatcaac tacacgaacg tctgcatcta ccgctgcgcg ttctgcgcgt
    11041 tctaccggcc cgcgggcgac ccggagggct acgtcctctc cttcgacgag atcgggaaga
    11101 aggtggagga gacgatcgct ctcgggggca cgggcattct cctccagggc ggggtgcacg
    11161 ccgagctgtc gttctcgttc tacgaggacc tcctctcgtt cctccgcgcg tcgtacccgc
    11221 gcgtccatcg gcacacgttc tcggcgcccg agatccactt cctcgcgaag aaggagaaga
    11281 cgacgccgga cgacgtgctc gcgcgtctgc agaaagccgg catgcagtcg ctcccgggag
    11341 gaggcgccga gatcctcgag gacgaggtgc gtaaacgcat ctggtccctc acgaaggcgc
    11401 ccacggagaa gtgggcggaa gtccaccgcg ccgcgcaccg gctcgggatg ccgacgaccg
    11461 cgacgatgat gttcggcgtc ggggagacgc acgagcagcg cctccaccac ctcgacgtcg
    11521 tccgccgcgc gcaggacgag tcgctcgccg agggcggcga gagcttcacg gcgttcatcc
    11581 cctggacgtt ccaggaggag aacacggccc tcgacggccg ggtcgagacg gcgggcggcg
    11641 cggactacct ccgcacgctc gcgatcgcgc ggctctacct cgacaacgtc ccgcacgtgc
    11701 agggctcgtg gctcacgcag ggcgcgaaga tcggcagcgt cgccgcggcg ttcggcgcgg
    11761 acgacctcgg ctcgatcatg atcgaggaga acgtcgtcgc ggcggccggc tcgcacaacc
    11821 ggatggcggg cgagggcgcc atgcgcgcgc tcatcgaggg ctcgggcttc acgcccgtca
    11881 aacgcggctc ggtctacgac cgctgccgcg acgcctgctg ccgggacctg cccgcgctcg
    11941 cctgagggcg ggcgcccttc accctcaggc caggtcgaag accaggaact ccgcttcgcc
    12001 gcccggcgcc ctgaacgtga aggccgtctc ctccgacgcg gagaggccgt caccggccga
    12061 cagcgacgtc ccgttgacct cgactttgcc gcggagcgcc tgtacccaga ggtgccgctc
    12121 cttcggcagc gtgagcgacg tctcggcgcc cttctcgagg acggaggcgt agagccgcac
    12181 gtcctgccgg atggcgagcg agccgtcggc gccgtccggc gagacgacga gccggagccg
    12241 gttcgtcttc tcggctttct cgaagcgctt ctcggcgtac ttcggcttgt ggcccgcccg
    12301 gtccggaagg atccagatct ggacgaagtg gacgggctcc gtgtcgctcc cgttgaactc
    12361 gctgtgcatg acgccggtgc cggcgctcat gtactggacg tcgccgggga cgatcgtcga
    12421 accgttcccc atcgagtcct tgtgcgcgag cgcgccctcg aggacgtacg acacgatctc
    12481 catgtcgcgg tggccgtgcg tcccgaagcc ctcgccgggc tggacggtgt cgtcgttcag
    12541 gacgcgcagc gtgcggaagc ccatgtgcct ggggtcgtag taatcggcga acgagaagga
    12601 atgccacgtg tcgagccagc cgtggttggc gtggccgcgc tctcgggcgg gacggacggt
    12661 gatcatcggg actccgaccg ggaggggacg tgaaaccggc ccctccggcg actgtttggt
    12721 gcgacccgga caacgcggga gggggagggt ttattcccta cagcagcttc tccgagatgc
    12781 tcttggcctg caggaagagc ccgaggtagt cgcggccgcc ggccttgctg tccgtgccgg
    12841 acatgtcgaa gccgccgaac gggtggacgc cgacgagcgc gcccgtgcac ttcctgttga
    12901 tgtagaggtt cccgcagtgc aggcgcctct tggcctcggc gatccgatgg cggtcgcggc
    12961 cgaagtacga gcccgtgagg ccgtactcgc tcgcgttcgc gtacgcgatc ccggtctcga
    13021 agtccttcgc ctcggtgatg ccgaggacgg gtccgaagat ctcttcctgg aagatgcgcg
    13081 ccgtgggctt cacgtcgtag aagaccgtcg gggcgacgaa gttgccgacg cggccgtgcc
    13141 aggtcccgcc cgttccgagc ttcgactcgc gccggccgac ccggatgtag tccgtgaccg
    13201 tcttgaactg gcgctcggag gcgacgggac ccatgaagtt cttggggtcg cgcgtcgggc
    13261 cctgcgtgag cttggccgtc ttcgcgacga ccgcgtccac gaagcgcttg tacaggcgct
    13321 tgtgcacgat cgcgcgcgag caggccgagc acttctggcc ctggaagccg aacgccgatg
    13381 cgacgacctg cgagaccgcg aagtcgaagt ccgcgtcctc gtccacgaag atgaagtcct
    13441 tgccgcccat ctcgaggacg acgcgcttga tccagcgctg acccttcgcg accgcggccg
    13501 ccttctggtt gatcgtgagg ccgacctgct tggagcccgt gaacgagatg aagcgcgtct
    13561 gtgcgtgctc gacgagaagg tcgcccacct cgctgccggg ccccggcagg tagttgagcg
    13621 cgccggcggg gacgccggcc tcctcgagaa gcgtgaacac gcgccagccg atgagcggcg
    13681 tgtcggacgc gggcttgagg acgacgccgt tgccggtgac gagcgcggcg gcggtcatgc
    13741 ccgtgaggat cgcgttcggg aagttccacg gcgggatcac cgcgcccgcg ccgagcgcga
    13801 cgtagagcgt ctccgggaat tcgcccggat acggcgtgac gggaatctct ccgccccagc
    13861 gcagcatctc ccgcgcgtag aactcgagga agtcgatcgc ctcggcggtt tcgacgtcgg
    13921 cctcgggcca ggacttgccg atctcgagga ccatcgtggc cgagaactcg tgctttttgg
    13981 cccgcatgcg agcggccgcg cgcaggagga ccgacgcgcg ctcgcgcgcc gggagctcgg
    14041 cccacggctt ctgcgccttg acgaccgcct cgaccgcctt gagggcgatc ctgcggtcgg
    14101 cgagatggtg gcgggacagc acctccgagc ggtcggaggg gttcgtgttc tcgaggagac
    14161 ggccggtctt gagacgccgt ccgcccacga cgacggggat ctcggcgccc ttctggcggt
    14221 cgacccggtt gagggccgcc tcgaacgcgg agcgctcctc ggcgcggctg aagtcggtga
    14281 agggttcgtt gcggaagtct tcaagcatgg gagggggatt ctatcgttac actcggggag
    14341 gaaggggtcc tcatgaccgt ggaagagttg aaggggaagc tggacgcggg agaagcggtg
    14401 tgcgttctcg acgtgcgcga ggcgcgcgag ctcgaggtcg cccgctatcc ctttacggtg
    14461 ctgcacattc cgatgagtca ggtgccggcg cggctggacg agatcccgaa ggaccggacc
    14521 gtcgtgtgcg cgtgccgcag cgggagccgg agcgcgcaca tcgcggcgtt cctgaaatcc
    14581 cacggccgcg acgccgtcaa tctcgacggc ggaatcctcg cctggtccgc gcacatcgat
    14641 ccttcgattc cgcggtactg acccgtcgtt ccgggcggcg gtccccacat ttgatttgtg
    14701 tccatcacca tgacaaagtt ggttatcggg gaggttcgac ccatggataa gaacgtcgcg
    14761 aagattcgcg gcttcgtcac cgacttgctc aggaagaaca aggcgctcac ggagcgcgag
    14821 gtaaaagcgg ccatcggccg cgcgttcaag ctgaacccga agacgatcag cgccggcgtc
    14881 atccgcgacg tccgcaagaa actcggaatc gaccggccgg gagcgatcgc gttcgcgaag
    14941 gccctgctgg acaagaaccc cgtcctcgag gcgaagaagg tcatcgagga ggtcggcacg
    15001 cggttcggcg tccgcctcgg cgctccggac gtctcgcggc tgcgtcccgt cggggcgcgc
    15061 cggcgcctcg gcggcccgag acccgaccgc gtggcgatgc ggaagaccgc gacgcgcggc
    15121 gga
//
LOCUS       DAOMFY010000070        15019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1222_length_15019_cov_6.657378, whole genome shotgun sequence.
ACCESSION   DAOMFY010000070 DAOMFY010000000
VERSION     DAOMFY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1222_length_15019_cov_6.657378"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLB01_07750"
     CDS             <1..233
                     /locus_tag="PLB01_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HQQ77235.1"
                     /translation="GVSRTDADAALAALDPADEARSLRLALEKRARALPAALTPQARS
                     KKLFDHLVRRGFAPSAVLEALRTKGEPTDDDL"
     gene            220..2991
                     /gene="alaS"
                     /locus_tag="PLB01_07755"
     CDS             220..2991
                     /gene="alaS"
                     /locus_tag="PLB01_07755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQQ77236.1"
                     /translation="MTTSEGWTSRRVRETFLSYFEGKGHARVASSSLVPAEDPTLLFT
                     NAGMNQFKDLFLGREKRAYVRATSSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFS
                     FGDYFKKDAIDFAWELVTSKDFYGLDPKRLWATVYTSDDEAAELWGRYLPKERIARFG
                     EKDNFWSMGDTGPCGPCSELHYDQGPSVPGDATPNGNGDRVMEIWNLVFMQFDRDAAG
                     NQTPLPKPSVDTGAGLERITAILQGKNNNYDTDLFEPIIRQIERLSGHKYVGGMVVED
                     APFRVIADHARAATMLMLDGVIPSNEGRGYVLRRIIRRAARYGRRLGLTPAQPLLANL
                     ASSVAHTFEGIHIEAAAAAAALVPIQDRLHAEEQLFSKTMSVGADRVGEAISAARREG
                     ETRLQGQTVFRLYDTFGVPLETIEEIASDEGVHVDTQGFDRELTAARERSKGASKFEG
                     ESGLPADDQLDPSWSTEFRGYPEQDFVRLEGAKVLGLFSEKGGEKIGFLRKGEKGWCV
                     LDRTVFYPEGGGQVADQGILSWSPDGTATVLDTRRAPNGRTILHLLRNDSGTLAPAQL
                     VSVSVDEWTRRRTQANHTGTHLLHAALRTVLGESVRQMGSLVAPDRLRFDYAASRPTT
                     AEEIREIERLVNEEVLRDREVSKAVMSMDEAKKKGAMMFFGEKYGERVRVVDVPGFST
                     ELCGGCHVGRTGEIGAFKILSDKGLAAGVRRIEAATSLNAVERLQKDEEILGQLTHAA
                     NVPLDELPAKFKEIQDLLRRKEKEVAALKRDLALAGAGASGAQPGAGASGHSPSKKSS
                     SSSESSDEGIEDVGGVKILARRVPSLPTNELRNLADTFRNKLKSGVVLLGTEFEGKVT
                     LLAAVTADVVGRVSAHDLAQTMAPLVGGKGGGKPDLAQAGGKDAAAIDPALAAGVARV
                     RERLAGQAP"
     gene            3229..3564
                     /locus_tag="PLB01_07760"
                     /pseudo
     CDS             3229..3564
                     /locus_tag="PLB01_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            3766..5049
                     /locus_tag="PLB01_07765"
     CDS             3766..5049
                     /locus_tag="PLB01_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77237.1"
                     /translation="MASRGSETTRAFRPAPFFDHGLFLLHLNRGKEEMARGHHEAARR
                     EFDEAQRLRPQDPEVLLNLSITLFHLGQFGPAEAKTRELLQEHDDSVPLLFNLGLILF
                     KSGRDAEAKEPLEKVLALAPAHRKAHLTLGLIAQRAEAYEAAQRHFRLAGAELKDGSE
                     GDDSVARTARAAAAEVRAAALTPAANPDVTRPAPVVRDNTRPTPVVKPEPLDSLAAGG
                     KRNLVSSGVRSRSGSTPRADLAPALGPFTPKPGGFLSASCAGGLRVRRGVVVGRQGVP
                     SLVTDGRLDGPLAGILVTASGEGTLLLADQGRVPYLKALANEFLSVETSRLLGFDEAL
                     TYREDPAFEYRDKIVQPFLKLFGTGTVALAVVSEPARFEVERQNPFTIAARAVVAYGG
                     DLTPELLEESDPIAGLGAGPVLRFVGAGFVLADAR"
     gene            complement(5117..6532)
                     /locus_tag="PLB01_07770"
     CDS             complement(5117..6532)
                     /locus_tag="PLB01_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HQQ77238.1"
                     /translation="MSAPPIVFGTDGWRGQIARDLTFASVLRVVDAAGAWNVSPENAE
                     PGDPWTVPLVHDTRFLSPELAAEGAARLASRGFRVLLTDRPVPTPCASWHVKSRGLRA
                     GLAITASHNPALWNGVKVKSWFGGSATPATYSAVARYADRPLPARAGGAVETLDLHAG
                     YRDAIAACVDLTTIRGAGLNVLFDSMHGAAGTLLAEIVGAGHRTRVTTLRAARDPLFG
                     GVNPEPIPEHLGASREELARGTYDVALASDGDGDRLGVLDASGAFVTPHRILALLAES
                     LTARGRISGGIAKTFSTSLLLDRVAARMGVPLHVTPIGFKHIAEKMMSGEVAIGGEES
                     GGLGVSFYLPERDGVLSALLVLEAIAHSGGSFDALLKRQDAAHGAFAYGRRDLHLPMP
                     LLRAFVADLRAAPPATRAGQAVTGVADLDGVKLLFGERGWLLHRLSGTEPIIRVYAEH
                     EDEKVVERLLAETVDELRRRA"
     gene            6608..7183
                     /gene="pgsA"
                     /locus_tag="PLB01_07775"
     CDS             6608..7183
                     /gene="pgsA"
                     /locus_tag="PLB01_07775"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQQ77239.1"
                     /translation="MFFNVPNALTLLRIFLVPLLVVLLLTKLPAREYMALAVFLLAAI
                     TDWADGWFARKYKKVTTVGMLLDPIADKLLVSAALISLVDLQEADAWAVCIIIGREFA
                     VSGLRSIASTHGITIAASKLGKWKMGTQIVGISLMILGAKLDDIGLWRKTGRAALYVM
                     MAMALISGFDYFRKFLRPLLAMEREPGGGSP"
     gene            complement(7180..7878)
                     /gene="trmB"
                     /locus_tag="PLB01_07780"
     CDS             complement(7180..7878)
                     /gene="trmB"
                     /locus_tag="PLB01_07780"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQQ77240.1"
                     /translation="MSRPSIRRALKRSGAVMPPRTPRTGPRNLGTAPPPKIFLAGEGA
                     DAGVVDFAAIFGRKAPVEMEIGVGKGRFLLGAAAANRDRDFFGLEIEAEYAAIVRVRA
                     ERASLPNVRVERLDGKAFVASRLAPGCLAALHVYFPDPWPKKRHHKRRLVDPGWASAA
                     ARALEPGGLLRVASDHADYWTVIDAVLSAEPLLAKLAPEETGEWSTGTNYELKFLKTG
                     RPIRKAVFRRVAVS"
     gene            complement(7880..8173)
                     /locus_tag="PLB01_07785"
     CDS             complement(7880..8173)
                     /locus_tag="PLB01_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQQ77241.1"
                     /translation="MPKLVFLNEGRRAEVAAGSTILAAADALRVPIGRVCGGGGTCST
                     CRVEVVSGAGNLSPIEENEIAYGLGPNVRLACQARVLGDVGVRVLVIPRALVV"
     gene            complement(8173..9159)
                     /locus_tag="PLB01_07790"
     CDS             complement(8173..9159)
                     /locus_tag="PLB01_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HQQ77242.1"
                     /translation="MTLVPGDGIGPEITAAVVEILAAAGAPITWESHDAGMAAVAATG
                     NPLPPEIVTSVQRTKVCLKGPVTTPVGGGYKSVNVSLRQALDLYANLRPVKNLPNVAS
                     RYGDLDLVVVRENTEDLYSGIEHVVVPGVVESIKIITEKASTRIARFAFEYARTHGRR
                     RITAVHKANIMKLSDGLFLDCFRAVAKDYPDIQADDVIVDAACMKLVMNPKQFDMLLL
                     ENLYGDIISDLASGLVGGLGITPGANLGTHCAVFESVHGSAPDIAGKGIANPTALLLS
                     ATMLLRHIGEDARAARIEKALNAAMADGSAKTRDVGGTATTAAYAKAVIARL"
     gene            complement(9177..10463)
                     /locus_tag="PLB01_07795"
     CDS             complement(9177..10463)
                     /locus_tag="PLB01_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQQ77243.1"
                     /translation="MNEGLLLGLYVLCAMGSAFFSAGETAITSVTDSAIFRMKSEGRR
                     GAERLERLRADLPKTLGTLLVGNTLANAAAGSLGAGLAISAFGEKWGMLVATLLTSVL
                     LLVVAEVTPKTLAARRPEALSLLFVGPIETCVSLLTPATALLSAVARWILGPFGVEES
                     ADVTEEDVKSVISLSREQGGLKREESDLLHAVLAFGDTTVRDAMVPRAKIVSLDVTSS
                     FDRVEAVCREHRYSRYPVTRGHEDDVVGILHVKDLFDVSDAEEATFDLSRYLRPAVFV
                     PELKRAEDLFREMRRRRFHMAIVVAESGAVAGLVTLEDLIEQIVGDIFDEHDEPARPP
                     VAEGTSLLVEGAYPLASLERDLSVSFDDPEAETVAGFLLRKFGRIPRAGARTHAGGVE
                     FLVERATPRAIERVRILRPDAPRPADDADNRKASPT"
     gene            complement(10460..11287)
                     /locus_tag="PLB01_07800"
     CDS             complement(10460..11287)
                     /locus_tag="PLB01_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQQ77244.1"
                     /translation="MSAPGTLLVVAGVLVREGRLLLARRPAGRPWAGLWEFPGGKVED
                     GETPEDALVREWREEMDVTPVGLIPFSFSTIAGADRGDSSRHVTLLFFEVNGVVGEPR
                     AVTVDAVRWCSAAEARLLPTPPADAPALVRLLEKAAGTGAFADTESAAGLALVREHAA
                     RSPFIEESESLSSLRALAFRKPRPDGAGTVSGLLVATQDGGARAYRNVCPHVPIPLDR
                     GGEPLLTEEGFLACRNHGALFDVENGLCVAGPCAGESLEAIPVEPRGAGWALSGGRA"
     gene            complement(11284..12231)
                     /gene="queG"
                     /locus_tag="PLB01_07805"
     CDS             complement(11284..12231)
                     /gene="queG"
                     /locus_tag="PLB01_07805"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQQ77245.1"
                     /translation="MARVPRRPPPPEPSDPAALSRAAVAFACARGFAAAGVLAASRPL
                     TWETYRDWLHEGRHAEMAYLERDAAARMRFDSILPFAKSVLAVARAVPGTGRGNVAAY
                     ARGEDYHRVVRRHLKGVIDDLKPLAPRGSHFRVCVDTAPLLEREIAVRAGLGFVGKNG
                     MLIVPGVGSHVVLGEILTDVTLAPSASPFADGADRCGSCTACLEACPTQAFVSPRVLD
                     SAKCLSYLTIEKRGPLTDVEESALGGRLFGCDDCQDVCPFNAALDPAGPARGPAASLD
                     PEEILALDDDGFRARFFGSAIWRATRSGLVRNARAAVKR"
     gene            12286..13383
                     /locus_tag="PLB01_07810"
     CDS             12286..13383
                     /locus_tag="PLB01_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQQ77246.1"
                     /translation="MRENRAAHLYPVLFLLVLLLAVGALSTIVLRPFFDGILWASVVA
                     VALWPLWTKVRGRAGKRRGLAAGLFTLAVALVVLLPAVVLGAAILNQAAGAATAIGLR
                     LRESDVHSWNDVLAIPGVERAMAWVRDTAGVSAEDLQGKALEFARTASSRLAAAGGSA
                     VLSFLDILVTFILTLFLLFFFLRDGEDMVEALTDLIPIDDAERRRIVRALGSMLESIF
                     KGSLLCAMIQGTSGALAWVAAGLPSAILAGVAMSILSLLPVGGTAIVWGPALVVLFVK
                     GRTGVAIAFLLWNVLVTSTLADNVLKPMLIGQKGGELSTLLVFLGVFGGLSAFGLLGV
                     FVGPMALAVAVMLVRVLRDIAGASREASAEA"
     gene            complement(13367..13489)
                     /locus_tag="PLB01_07815"
     CDS             complement(13367..13489)
                     /locus_tag="PLB01_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77247.1"
                     /translation="MQKLARLARTYDYLVPWVFLAVIGGATLAVLQVLVVRLLH"
     gene            complement(13504..14610)
                     /locus_tag="PLB01_07820"
     CDS             complement(13504..14610)
                     /locus_tag="PLB01_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77248.1"
                     /translation="MRNHRRPLAVAAAASLVAAALAGLAAIAADGPAAGSSEGTLSPL
                     SYEGEKHLANVRQLTFGGENAEAYWSADGRKLIFQSTRPPYACDQIFEMEVAAPEKTT
                     RLLSTGKGRTTCAYFFPDGKRYLYASTHLGGDSCPPPADMSQGYVWALYPEYDIFSAN
                     ADGTGLKRLTSAPGYDAEATISTDGKRIVFTSARDGDLELYAMDADGGNVKRLTNTVG
                     YDGGAFFSDDGQWLVYRAHHPTEPKAVESFKSLLMRSLIRPSKLEIRVMRADGTGDRQ
                     VTSLDAASFAPFFFRGGHDRILFCSNTADPKSRNFDLWAVNADGSNLERVTFNPTFDG
                     FPMFSPDGKRLAFCSNRHNAKAGDTNVFVADWVP"
     gene            14636..>15019
                     /locus_tag="PLB01_07825"
     CDS             14636..>15019
                     /locus_tag="PLB01_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77249.1"
                     /translation="MSPNKDDAAGRDAAALARDLVTQISAISASLSSGLESRAGAAHR
                     AAGAGAGPAYEWPRALLENAGRFVSPVLPAEAALRRIKAAVLRLLRIVTRDQTTFNSA
                     LLEAVRGCPSVRAVVFTAATSLPPPG"
BASE COUNT         2264 a         5083 c         5443 g         2229 t
ORIGIN      
        1 agggcgtgtc gcggacggac gcggacgccg ccctcgcggc gctcgacccg gcggacgagg
       61 cgcggagcct ccggctcgcc ctcgaaaagc gggcccgggc gctgccggcc gcattgacgc
      121 cgcaggcgcg gtcgaagaaa ctcttcgacc acctcgtgag gcggggcttc gcgccctcgg
      181 ccgtcctcga ggcgctccgc acgaaaggcg aaccgaccga tgacgacctc tgaaggctgg
      241 acctcacgca gggtccgcga gacgtttctg tcctacttcg agggcaaggg ccacgcgcgc
      301 gtggcgtcct cctctctcgt ccccgccgag gacccgacgc tcctcttcac gaacgcgggc
      361 atgaaccagt tcaaggacct ctttctcggg cgggagaagc gcgcgtacgt gcgtgcgacg
      421 tcctcccaga agtgcgtccg ggccggcggc aagcacaacg acctcgacaa cgtcgggtac
      481 acggcgcgcc accacacgtt cttcgagatg ctcgggaact tctcgttcgg cgactacttc
      541 aagaaggacg cgatcgactt cgcgtgggag ctcgtgacgt cgaaggactt ctacgggctc
      601 gacccgaagc ggctctgggc gaccgtgtac acgagcgacg acgaggcggc cgagctctgg
      661 ggccggtacc tcccgaagga gcgcatcgcg cgcttcggcg agaaggacaa cttctggtcg
      721 atgggagaca cgggcccgtg cgggccctgc tcggagctcc actacgacca ggggccgtcg
      781 gtccccggcg acgcgacgcc gaacgggaac ggcgaccgcg tcatggagat ctggaacctc
      841 gtcttcatgc agttcgaccg cgacgcggcg gggaatcaga ccccgctccc gaagccgagc
      901 gtcgacaccg gcgcgggcct cgagcgcatc acggcgattc ttcaggggaa gaacaacaac
      961 tacgacacgg acctgttcga gccaatcatc cgacagatcg agagactgtc ggggcacaag
     1021 tacgtgggag ggatggtggt agaagacgct cccttccgcg tgatcgcgga tcacgcgcgg
     1081 gcggcgacga tgctcatgct ggacggcgtg attccttcga atgaaggaag gggatacgtc
     1141 ctgcgccgaa tcatccggcg tgccgcgagg tacggccgtc ggctcggctt gacacccgcg
     1201 cagccgcttc tcgccaatct cgcctcatcc gtcgcacaca ccttcgaagg aattcacatc
     1261 gaagcggctg ctgctgcggc cgcccttgtg ccgatccagg accgtctgca cgcggaggag
     1321 cagctcttct caaagaccat gtccgtcggc gccgaccgcg tcggcgaagc gatctccgcg
     1381 gcgcggcggg agggagagac aaggctccag ggtcagacgg tctttcgcct ctacgacacg
     1441 ttcggggttc ccctggagac gatcgaagaa attgcttctg atgaaggtgt ccacgtcgac
     1501 actcagggct tcgaccgcga gctcaccgca gcccgtgagc ggagtaaagg agcttcgaag
     1561 ttcgagggag agagcggcct gccggcggac gaccagctcg acccctcgtg gagcacggag
     1621 ttccgtggct atccggagca ggatttcgtg cggctcgagg gcgcgaaggt gctggggctg
     1681 ttctcggaga agggcggaga aaagatcgga tttcttcgaa agggggaaaa gggctggtgc
     1741 gttctggacc gcaccgtctt ctatccggag ggcggcggac aggtcgcgga ccagggcatc
     1801 ctgtcgtggt ctccagacgg caccgcgacg gtcctcgaca cgcgccgcgc gcccaacggc
     1861 cggaccattc ttcaccttct aagaaacgac agcggcaccc ttgcaccggc gcagctcgtg
     1921 agcgtctctg tcgacgagtg gacgcgccgc aggacgcagg cgaaccacac gggcacgcac
     1981 ctcctccacg cggcgcttcg caccgtgctc ggcgagagcg tccggcagat gggctcgctc
     2041 gtcgcgcccg accgcctgcg cttcgactac gcggcctcgc gcccgaccac ggccgaggag
     2101 atccgggaaa tcgagcgtct ggtgaacgag gaggtcctgc gcgaccgcga ggtgtccaag
     2161 gccgtcatgt cgatggacga ggcgaaaaag aagggcgcga tgatgttctt cggcgagaag
     2221 tacggcgagc gcgtccgcgt cgtggacgtg cccggcttct cgacggagct ctgcggcgga
     2281 tgtcacgtcg gccgcacggg agagatcgga gcgttcaaga tcctctcgga caagggcctc
     2341 gcggccggcg tccgccgcat cgaggcggcg acgtcgctga acgcggtcga gaggcttcag
     2401 aaggacgaag agatcctggg acagctcacg cacgcggcga acgtgcctct cgacgagctc
     2461 cctgcgaaat tcaaggagat tcaagatctt cttagaagga aagaaaagga ggtcgccgcg
     2521 ctgaagcggg acctggccct cgcgggcgcg ggcgcctcgg gcgcgcaacc gggggccggc
     2581 gcctctggac attcaccttc taagaagtct tcctcttcct ccgagtcttc cgacgaaggt
     2641 atcgaggacg ttggcggcgt caagatcctc gcgcggagag ttccgtccct gcccacgaac
     2701 gagctgcgca acctcgcgga cacgtttcga aacaagctga agtcgggcgt cgtgcttctc
     2761 gggacggagt tcgaggggaa ggtcacgctg cttgcggccg tcacggcgga cgtcgtcggg
     2821 cgcgtgtccg cacacgacct cgcgcagacc atggcgccgc tcgtcggggg gaagggcggc
     2881 ggcaagcccg atctcgcaca ggccggcgga aaggacgcgg ccgccatcga tccggcgctc
     2941 gccgccgggg tggcccgggt gcgcgaaagg ctggccggac aggcgccttg agcgttcgtc
     3001 tataatttgt aggccatgtc cttcggacgt tttgccgccc ggggagcgtt cgcggtgtcg
     3061 ctctgcgcga ccgccgctct cggagacgtg catctcgtcc gccaggcgga cggctcggcc
     3121 ctcatcttca acgacgcgat cgggagcgga tggcgcgtga acggcggcgc tccgtccgac
     3181 tcctatctcg tggaccgccg ggcgacgccc accccgttcg acccgatcat cgaggccgag
     3241 gcccgggccg agggcgtgga cgcgacgctc gtcaagagca tcatgctcgt cgaatccaac
     3301 ttcaatccca gggccgtttc gaagaagggc gcccgggggc tgatgcagct gatgcccgtc
     3361 gccgcgcagc gcttcggcgt ctcggatcgc ttcgacgccg agcagagcat ccgcggcggc
     3421 gtgcggtacc tcgcgaacct gctcgccctg ttcaagggcg acgtcgcgtt ggccgtcgcg
     3481 ggctacaacg cgggcgagaa cgcggtcgca aggcacgcgg ggatcccgcc ctacgcggag
     3541 acgcgcgagt acgtgaggcg cgtcctcgtc gcccatcgcg gaacgtcggt gcccgcaatc
     3601 ggcggggcct tccggggcgt cccgacgggc tccgtccgga tggcgacgcg ggcgacggcc
     3661 gtgaaggtct cgcgcgtgga cgggaccgcc gttttttcga acatgggcga cgtcgcctcg
     3721 gcgcgctccg ctcccgtcct cggacgggtg cagtaagggg acggcatggc gtcgcgcggc
     3781 tccgaaacga cccgggcgtt ccgccccgcg cccttcttcg accacggcct cttcctcctg
     3841 cacctgaacc ggggcaagga ggagatggcc cgcggacatc acgaggccgc acgccgcgag
     3901 ttcgacgagg cccagcgtct gcgcccgcag gatccggagg tccttctgaa cctctcgatc
     3961 acgctctttc acctcggaca gttcgggccc gcagaggcga agacgcgcga gctcctccag
     4021 gagcacgacg attccgttcc gttgctcttc aacctcggat tgatcctctt caagtcgggc
     4081 cgcgacgccg aggcgaagga gccgctcgag aaggtcctcg cgctcgcccc cgcgcaccgg
     4141 aaggcgcatc tgacgctcgg gctcatcgcg cagcgcgcgg aggcctacga ggccgcgcag
     4201 aggcacttca ggctggcggg cgcggagctc aaggacgggt ccgagggcga cgactcggtc
     4261 gcgcggacgg cgcgcgcggc cgccgccgaa gtccgcgcgg cggccctcac gcccgcggcg
     4321 aacccggacg tcacgcgtcc ggcgccggtg gtgcgcgaca acacgaggcc gaccccggtc
     4381 gtgaagcccg agcccctcga ttcgctcgcc gcgggcggga agcgaaacct cgtcagctcg
     4441 ggcgtccggt cgcgctcggg gagcaccccg cgagcggacc tcgctccggc gttgggcccc
     4501 ttcaccccga agcccggtgg attcctgtcg gcatcctgcg cgggcgggct gagggtccgc
     4561 cgcggcgtcg tcgtggggcg tcagggcgtc ccgtcgctcg tgacggacgg ccgcctcgac
     4621 ggcccgctcg cgggcatcct cgtgacggcg tcgggagagg gcacgctcct cctcgcggat
     4681 cagggccgcg ttccgtacct gaaggcgctc gccaacgagt tcctgtcggt cgagacgagc
     4741 aggcttctcg ggttcgacga agcgctcacg taccgcgagg acccggcgtt cgagtaccgc
     4801 gacaagatcg tccagccgtt cctgaagctc ttcggcacgg gcacggtcgc gctcgccgtc
     4861 gtgtcggagc ccgcgcgctt cgaggtggag cggcagaacc cgttcacgat cgcggcccgc
     4921 gcggtcgtgg cctacggggg agacctgacg cccgagctcc tcgaggagtc ggacccgatc
     4981 gccggcctcg gcgcggggcc ggtcctgcgg ttcgtcggcg ccggattcgt cctggccgac
     5041 gcgcgctgag cggggcaggt ccgaagagca ccgaagattt cttagaaggt aatagggcag
     5101 gggtcaacgg ccgtcgtcaa gcgcggcggc gaagttcgtc gactgtttcc gcgaggagcc
     5161 gctcgacgac cttctcgtcc tcgtgctcgg cgtagacgcg gatgatgggc tccgtgcccg
     5221 agaggcgatg gaggagccag ccgcgctccc cgaagagcag cttgacgccg tcgaggtccg
     5281 cgactcccgt cacggcctgc ccggcgcgtg tcgcgggcgg cgcggccctg aggtccgcga
     5341 cgaacgcccg gagaagcggc atcgggagat ggaggtcgcg gcgcccgtat gcgaaggcgc
     5401 cgtgggccgc gtcctgccgc ttcagaagtg cgtcgaacga gccgcccgag tgcgcgattg
     5461 cctcgaggac gagaagcgcc gagagaactc cgtcgcgctc cgggaggtag aacgagacgc
     5521 cgaggccgcc cgattcctcc ccgccgatcg cgacttcgcc ggacatcatc ttctcggcga
     5581 tgtgcttgaa gccgatcggc gtcacgtgga gcggaacgcc catgcgcgcc gccacgcggt
     5641 cgaggagcag cgacgtcgag aacgtcttcg cgatgcctcc tgagatccgc ccgcgagccg
     5701 tgaggctctc ggcgaggagc gcgaggatcc ggtggggcgt cacgaacgcg ccgctcgcgt
     5761 cgaggacgcc gaggcggtct ccgtcgccgt cgctcgcgag ggcgacatcg tacgtgccgc
     5821 gtgccagctc ctcgcgcgag gctccgaggt gctcgggaat gggctcgggg ttcacaccgc
     5881 cgaagagcgg gtcgcgagcc gcgcggagcg tcgtgacgcg ggtcctgtgg ccggccccga
     5941 cgatttccgc gagcagcgtt ccagccgcgc cgtgcatcga atcgaaaagg acgttcaggc
     6001 ccgcaccccg gatcgtggtg aggtcgacgc acgccgcgat cgcgtcgcga tagccggcgt
     6061 ggaggtcgag cgtttcgacg gccccgccgg cccgggccgg gagagggcga tccgcgtagc
     6121 gcgcgacggc ggagtaggtc gcgggcgtcg cgctgccgcc gaaccacgac ttcaccttga
     6181 cgccgttcca gagcgccggg ttgtgcgaag ccgtgatcgc gaggccggct ctcaggccgc
     6241 gggacttgac gtgccacgag gcgcagggcg tcggcacggg gcggtcggtc aggagcacgc
     6301 ggaagccccg agaggcgagg cgggccgcgc cctcggcggc gagttcgggc gaaaggaacc
     6361 gcgtgtcgtg gacgagcgga accgtccacg gatcgccggg ttcggcgttc tcgggcgaga
     6421 cgttccaggc gccggcggcg tcgacgacgc gcaggacgga cgcgaacgtg aggtcccggg
     6481 cgatctggcc gcgccagccg tccgtcccga agacgatggg gggcgcggac atgagcatgg
     6541 ggattctcgg ccccgggggg cgggctgtcc agcgcgggag gaacgcccgg actagaatcc
     6601 cgccgcggtg ttcttcaacg tccccaacgc cctgaccctt ctccgcatct ttctcgttcc
     6661 gctcctcgtc gtcctcctcc tgacgaagct gccggcgcgc gagtacatgg cgctggccgt
     6721 gttcctgctc gccgcgatca cggactgggc ggacggctgg ttcgcgcgca agtacaagaa
     6781 agtgacgacg gtcgggatgc tgctcgaccc gatcgcggac aagctgctcg tttcggcggc
     6841 gctcatctcg ctcgtggacc tccaggaggc ggacgcgtgg gcggtctgca tcatcatcgg
     6901 acgcgagttc gccgtttcgg gcctgcgctc gatcgcctcg acgcacggga tcacgatcgc
     6961 ggcgagcaaa ctcggcaagt ggaagatggg gacccagatc gtcgggatct cactcatgat
     7021 cctcggagcc aagctcgacg acatcggcct ctggcgaaag acgggccgcg cggccctcta
     7081 cgtcatgatg gcgatggccc tgatctccgg cttcgactac ttccggaagt tcctccgacc
     7141 gctgctcgcc atggagcgcg agccgggcgg cggttcgccc taggagacgg cgacgcggcg
     7201 gaagacggcc ttccggatcg ggcggccggt cttgaggaac ttcagctcgt agttcgtccc
     7261 ggtggaccac tcgccggtct cctccggagc gagcttcgcg aggagcggct ccgccgacag
     7321 gaccgcgtcg atgacggtcc agtaatcggc atggtccgac gcgacccgca ggaggcctcc
     7381 cggctcgagc gcgcgcgccg ccgcggacgc ccagccgggg tccacgaggc gcctcttgtg
     7441 gtgccgcttc ttgggccagg ggtccgggaa gtagacgtgc agggccgcga ggcaacccgg
     7501 cgcgaggcgc gacgcgacga aggccttgcc gtcgagccgc tccacgcgga cgttcggaag
     7561 gctcgcgcgc tcggcccgca cccgcacgat cgccgcgtac tccgcctcga tctcgaggcc
     7621 gaagaagtcg cggtcccggt ttgcggccgc ggcgccgagg aggaaacggc ccttgccgac
     7681 gccgatctcc atctcgacgg gcgctttccg cccgaagatc gcggcgaagt cgacgactcc
     7741 cgcgtcggcg ccctcgccgg cgaggaagat cttcggcggc ggggccgttc cgaggttgcg
     7801 cggccccgtg cgcggagtcc ggggcggcat gacggccccg gaccgtttga gcgcgcggcg
     7861 gatcgagggc cggctcacgt cagaccacca gcgctctcgg gatcacgagg acgcggacgc
     7921 cgacgtcgcc gagtacgcgc gcctggcagg cgagcctgac gttcggaccg aggccgtacg
     7981 cgatctcgtt ttcctcgatc ggggagaggt tcccggcgcc ggagacgacc tcgacgcggc
     8041 acgtcgagca cgtgcctccg ccgccgcaga cgcggccgat cggcacgcgc agggcgtcgg
     8101 cggcggcgag aatcgtcgag ccggcggcga cttcggcgcg gcggccctcg ttcaggaaga
     8161 cgagtttcgg catcagagcc tggcgatgac cgccttggcg tacgcggcgg tcgtcgcggt
     8221 gccgccgacg tcgcgcgtct tcgcgcttcc gtcggccatc gcggcgttga gggccttctc
     8281 gatacgcgcg gcccgcgcgt cctcgccgat gtggcggagg agcatcgtcg ccgagaggag
     8341 gagcgcggtc gggttcgcga tgcccttgcc cgcgatgtcc ggggccgagc cgtggacgga
     8401 ctcgaacacg gcgcagtgcg tcccgaggtt cgcgcccggc gtgatgccga ggccgccgac
     8461 gaggccggag gcgaggtcgc tgatgatgtc gccgtagagg ttctcgagca gcagcatgtc
     8521 gaactgcttc gggttcatga cgagcttcat gcacgcggcg tccacgatga cgtcgtcggc
     8581 ctggatgtcc ggataatcct tggccaccgc gcggaaacag tcgaggaaga ggccgtccga
     8641 gagcttcatg atgttcgcct tgtgcacggc cgtgatccgg cggcggccgt gggtccgggc
     8701 gtactcgaac gcgaagcgcg cgatgcgcgt ggaggccttt tccgtgatga tcttgatcga
     8761 ctcgacgacg cccggcacga cgacgtgctc gatgcccgag tagaggtcct cggtgttctc
     8821 gcgcacgacg acgaggtcga ggtccccgta gcgcgaggcg acgttcggga ggttcttcac
     8881 ggggcggagg ttcgcgtaga ggtcgagggc ctggcgcagc gagacgttca cgctcttgta
     8941 gccgccgccg acgggagtcg tgacggggcc cttcaggcag accttcgtgc gctggacgga
     9001 cgtcacgatc tcggggggca ggggattgcc ggtcgccgcc acggcggcca tgccggcgtc
     9061 gtggctttcc cacgtgatgg gcgccccggc ggctgcgagg atctccacga cggcggccgt
     9121 gatctcgggg ccgatgccgt ctccgggaac gagggtcacg atgcgtttct tcggggtcac
     9181 gttggtgagg cctttcggtt gtcggcgtcg tccgcgggcc gcggcgcgtc cggtcggaga
     9241 atgcgaacgc gctcgatggc ccggggggtg gcgcgctcga cgaggaactc gacgccgccc
     9301 gcgtgcgtgc gtgcgcccgc gcgggggatg cgtccgaact tgcgaagaag gaacccggcg
     9361 accgtctcgg cctccgggtc gtcgaacgag acggagaggt cccgctcgag ggaggccaga
     9421 ggatacgccc cctcgacgag gagggaggtc ccctcggcga ccggcggccg cgcgggctcg
     9481 tcgtgctcgt cgaagatgtc cccgacgatc tgctcgatga ggtcctccag cgtgacgagg
     9541 ccggcaacgg cgccgctctc ggcgacgacg atcgccatgt ggaagcggcg gcggcgcatc
     9601 tcgcggaaga ggtcctcggc gcgcttgagc tccgggacga agacggccgg ccggaggtac
     9661 ctcgagaggt cgaaggtcgc ctcctcggcg tccgacacgt cgaagaggtc cttgacgtgg
     9721 aggatcccga cgacgtcgtc ctcgtggccc cgcgtgaccg ggtagcggct gtagcggtgc
     9781 tcgcggcaga ccgcctcgac gcggtcgaac gacgaggtga cgtcgagcga gacgatcttc
     9841 gcgcgcggca ccatggcgtc gcgcacggtc gtgtccccga acgcgaggac tgcgtgcagg
     9901 aggtcactct cctcgcgctt gaggccgccc tgctcgcgcg aaagcgagat gacgctcttc
     9961 acgtcctcct cggtgacgtc cgcggactcc tcgacgccga acgggccgag gatccagcgc
    10021 gcgacggccg agaggagggc ggtcgcgggg gtcaggagac tcacgcacgt ctcgatgggt
    10081 ccgacgaaga gaagggacag ggcctcggga cggcgcgcgg cgagcgtctt cggcgtgacc
    10141 tcggcgacga cgagcaggag gaccgacgtg agcagggtcg cgacgagcat gccccacttc
    10201 tcgccgaaag cggagatcgc gaggcccgcg cccagcgatc cggccgccgc gttcgcgagc
    10261 gtgttcccga cgagaagcgt cccgagcgtc ttcgggaggt ccgcccgcag gcgctcgagg
    10321 cgctcggcgc cgcgccggcc ctcgctcttc atccggaaga tcgccgagtc cgtgacggac
    10381 gtgatcgccg tctcgccggc cgagaagaag gccgagccca tcgcgcagag cacgtagagg
    10441 ccgagaagca gtccctcgtt cacgcgcgac ctccggaaag agcccagccg gcgccgcgcg
    10501 gctccaccgg gatcgcctca agggactcgc ccgcgcacgg cccggccacg caaagcccgt
    10561 tctcgacgtc gaagagcgca ccgtggttgc ggcacgcgag gaagccctcc tcggtcagga
    10621 gaggctcgcc gccgcggtcg agcgggatcg ggacgtgcgg gcagacgttt cgatacgccc
    10681 gggcgccgcc gtcctgcgtc gcgacgagga ggcccgagac ggtgcccgcg ccgtcggggc
    10741 gcggtttcct aaaggcgagg gcgcggaggg aggagaggga ttcggactct tctatgaaag
    10801 gggagcgggc ggcgtgctcc cgaaccaggg cgaggccggc cgcggactcc gtgtccgcga
    10861 aggcgccggt gccggcggct ttttcgagga ggcggacgag cgcgggcgcg tccgcgggag
    10921 gcgtcggcag gaggcgcgcc tccgcggcgg aacaccagcg cacggcgtcg acggtcacgg
    10981 ctcgaggctc gcctacgacc ccattcactt cgaagaacag cagcgtcacg tgtcgcgatg
    11041 agtctccacg atcggcgccg gcgatcgtgg agaaagagaa cgggatgagc cccaccggcg
    11101 tgacgtccat ctcctcgcgc cactcccgca cgagcgcgtc ttcgggcgtc tcgccgtctt
    11161 ccacctttcc gccggggaac tcccagaggc ccgcccaggg ccggcccgcc gggcgccgcg
    11221 cgaggaggag ccgcccctcg cgcacgagaa ccccggccac gacgaggagg gtgccgggcg
    11281 cgctcaccgc ttcaccgccg cacgcgcgtt ccgcacgagc ccgctccgcg tggcgcgcca
    11341 gatcgcggaa ccgaaaaagc gcgcgcggaa gccgtcgtcg tcgagagcga ggatctcctc
    11401 cggatcgaga gacgccgccg gtccgcgtgc gggccctgcc ggatcgagcg cggcgttgaa
    11461 ggggcacacg tcctggcagt cgtcgcagcc gaacagcctt ccgccgagcg cggactcctc
    11521 gacgtccgtg agcggtccgc gcttctcgat cgtcaggtac gaaaggcact tcgccgagtc
    11581 caggacgcgc ggcgagacga acgcctgggt ggggcaggcc tcgaggcagg ccgtgcagga
    11641 cccgcagcgg tccgcgccgt ccgcaaatgg cgaggccgag ggcgcgagtg tgacgtccgt
    11701 gaggatctcg ccgaggacga cgtgcgagcc gacgcccggg acgatgagca tcccgttctt
    11761 gccgacgaag ccgaggccgg ctcgcacggc gatctcgcgc tcgaggagcg gcgccgtgtc
    11821 gacgcacacg cggaagtgcg agccgcgcgg cgcgagcggt ttcaggtcgt cgatcacgcc
    11881 cttgagatgg cgccgcacga cgcggtggta gtcctcgccg cgcgcatacg ccgcgacgtt
    11941 cccgcggccc gtcccgggga ccgcgcgcgc gaccgcgagg accgacttcg cgaacgggag
    12001 gatcgaatcg aagcgcatcc gcgcggccgc atcgcgttcg aggtacgcca tctccgcgtg
    12061 ccggccctcg tggagccagt cgcggtacgt ttcccaggtc agggggcgcg aggccgcgag
    12121 gacgccggcg gccgcgaacc cccgcgcgca cgcgaacgcc accgccgccc gcgaaagggc
    12181 cgcggggtcg gatggttcgg gagggggcgg acggcgcgga acacgcgcca cggcgggccg
    12241 cataaccgtt cagtctatca acgtcttgcg tatgatcgaa cggccatgcg agagaaccgg
    12301 gccgctcacc tgtatcccgt cctcttcctg ctcgtcctcc tcctcgcggt gggggcgctc
    12361 agcaccatcg tcctgcgccc gttcttcgat ggaatcctct gggcctccgt cgttgccgtg
    12421 gctctgtggc cgctctggac gaaggtccgc ggccgggccg ggaagcgccg gggcctcgcg
    12481 gccgggctct tcaccctcgc ggtggccctc gtcgtcctcc tgccggccgt ggtcctcgga
    12541 gcagcgattc tgaaccaggc ggccggtgcg gcgacggcga tcgggctccg gctgagggag
    12601 agcgacgtcc actcctggaa cgacgtcctt gcgattcccg gcgtcgagcg cgcgatggcc
    12661 tgggttcgcg atacggccgg cgtctcggcc gaggaccttc agggcaaggc gctcgagttc
    12721 gcgaggaccg cctcctcccg gctcgcggcc gcgggcgggt cggccgtcct ctcgtttctc
    12781 gacatcctgg tcacgttcat cctgacgctc ttcctgctct tcttcttcct cagggacgga
    12841 gaggacatgg tggaggcgct cacggacctc atcccgatcg acgacgccga gcggcgtcgg
    12901 atcgtgcgcg ccctcggaag catgctcgag tccatcttca agggctcgct cctctgcgcg
    12961 atgatccagg gcacgtcggg cgcgctcgcg tgggtcgcag ccggcctgcc ctcggcgatc
    13021 ctcgcgggcg tcgcgatgag catcctgtcc cttctcccgg tggggggaac ggcgatcgtc
    13081 tgggggccgg cgctcgtcgt cctgttcgtc aagggacgca cgggcgtcgc gatcgcgttc
    13141 ctcctctgga acgtcctcgt gacgtccacg ctcgcggaca acgtcctgaa gccgatgctg
    13201 atcggacaga agggcggcga gctctcgacg ctcctcgtct tcctcggagt ctttggcggc
    13261 ctctcggcct tcggcctcct cggcgtcttc gtggggccga tggcgctcgc cgtcgccgtg
    13321 atgctcgtgc gggtcctgcg cgacatcgcc ggtgcgtcgc gcgaggctag tgcagaagcc
    13381 tgacgacgag gacctgcagg accgcgagcg tggcgccgcc gattacggcg aggaagaccc
    13441 acgggacgag gtagtcataa gtccgcgcga gtctcgcgag cttctgcatg gcgctcctca
    13501 tccctaggga acccagtccg caacgaagac gttcgtgtcg ccggccttcg cgttgtgacg
    13561 gttcgagcag aacgcgaggc gcttgccgtc cggggagaac atcgggaacc cgtcgaacgt
    13621 ggggttgaac gtgacccgct cgaggttcga cccgtccgca ttcacggccc agaggtcgaa
    13681 attccggctc ttcgggtcgg ccgtgttcga gcagaagagg atccggtcgt gcccgccgcg
    13741 gaagaagaac ggcgcgaagc tcgcggcgtc cagcgacgtg acctggcggt cgcccgtgcc
    13801 gtcggcgcgc atgacgcgga tctcgagttt ggatggacgg atgagcgaac gcatcaggag
    13861 agacttgaag ctctcgaccg ccttcggctc cgtggggtgg tgcgcgcggt agacgagcca
    13921 ctggccgtcg tctgaaaaga acgcaccgcc gtcgtagccg accgtgttcg tgaggcgctt
    13981 gacgttgccg ccgtcggcat ccatcgcgta gagctcgagg tcgccgtcgc gggccgacgt
    14041 gaagacgatc cgttttccgt ccgtcgagat cgtggcctcg gcgtcgtagc ccggcgcgga
    14101 cgtgagccgc ttgagtcccg tgccgtcggc gttcgccgag aagatgtcgt actcggggta
    14161 gagcgcccag acgtagccct gcgacatgtc ggcgggcggc gggcaggagt cgccgccgag
    14221 gtgcgtcgag gcgtagaggt accgcttgcc gtcggggaag aagtacgcgc acgtcgttcg
    14281 gcccttgccg gtcgagagga ggcgcgtcgt cttctcgggc gcggccactt ccatctcgaa
    14341 gatctggtcg cacgcgtacg gcggccgcgt ggactggaag atgagcttcc tcccgtcggc
    14401 agaccagtag gcctcggcgt tctcgccgcc gaacgtgagc tgccggacgt tcgcgaggtg
    14461 cttctcgccc tcgtacgaga gcggcgagag cgtcccttcg gacgaaccgg ccgcgggtcc
    14521 gtctgcggcg attgcggcga gaccggccag ggcggcggcg acgagggaag cggcggcggc
    14581 gacggcgagg ggcctgcggt ggtttctcat gtttcgcgct taatataagc gacagatgag
    14641 cccgaacaag gacgacgcgg ccggccgcga cgcagccgcc ctcgcccggg acctcgtgac
    14701 gcagatctcg gcgatttctg cgagcctctc ctcgggcctc gaatcacgcg ccggcgcggc
    14761 ccaccgggcc gccggcgccg gggccggacc ggcgtacgag tggccgcgcg cgctcctcga
    14821 gaacgccgga cggttcgtga gcccggtcct tcccgccgag gccgcgctgc gccggatcaa
    14881 ggccgcggtg ctccggcttc tgcgcatcgt cacgcgcgac cagacgacgt tcaactccgc
    14941 tctcctcgag gccgtgcgcg ggtgcccttc ggtgcgggcc gtggtcttca cggcggccac
    15001 gtcgctgccg ccaccgggc
//
LOCUS       DAOMFY010000071        14997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1224_length_14997_cov_8.504952, whole genome shotgun sequence.
ACCESSION   DAOMFY010000071 DAOMFY010000000
VERSION     DAOMFY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14997
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1224_length_14997_cov_8.504952"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="PLB01_07830"
     CDS             <1..1591
                     /locus_tag="PLB01_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77250.1"
                     /translation="VVLRAPFSTAKQVESLHEALCFLRAWPDDAAVLARTEFLLARFA
                     RRPDLKRHRSALEDSGIAGTRTRFPFFAGTARWLAARWGGKLRVDWKAFEKGAALERL
                     LPLVSLWAETPALDELDLGTRGWVNRLKGPRETDAAFLVRRLATVGRGTFEKEWAWEG
                     LELPLVLEPGPGTPSRTNACLRTAPVVFQTRPLDRGRPDVRRELARPPLSVAAVPRRE
                     AERYLDLAREAMVARARDLDAFQWGDPQDVRLVTYPDGLAFAAIGVVPERRLLLESVY
                     GFLTLKNGVPIGYVLTSALFGSSEIAYNVFESFRGGESGLVYGKVLSMTRALFGSDVF
                     TIFPYQLGGYGNPEALQSGAWWFYRKLGFAPRAPRAVALMNEEERRMAADPKHRTSVP
                     DLKVLASENLYWFGGRVRSDAIGLVELPNVGLRVVDAVRSRFGSDRERAEAVLTAEAA
                     RLLGARTAAWSPAERLAFRRWAPLVSVLPGVEAWSPEEKRALAAVIRAKGGRRESDFV
                     RLFDAHRKLRKAVVELAKDPG"
     gene            complement(1588..3984)
                     /locus_tag="PLB01_07835"
     CDS             complement(1588..3984)
                     /locus_tag="PLB01_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQQ77251.1"
                     /translation="MVARPEDLLEGLNPEQAAAVSHGEGPLLVLAGAGSGKTRVLTRR
                     LAWLVAHGVPQEGIVAVTFTNKAAGEMKERVANLLGTERPRSYVGTFHSWGVRLLRRF
                     ADEAGLPRGFVVFDSDDQLAVVKRVMKEVAIPEKSATPRQIQSKISAAKNEGISPDDF
                     PRRFGDFLGSRLADVARRYEKTLRASQALDFDDLLVLSAKLVATNDAVRALLRRSIRH
                     LLVDEYQDTNRVQAKLVKLVTGEAGNLFVVGDEDQSIYRWRGADVSNILEFTRDFPTA
                     KTVRLERNYRSTAPILKAAGAVVAENRRRLGKTLKATKTGGEKVRLVAFDEERDEARE
                     IVARVAAARRARPQGEVAVLFRTNAQSRPFEDELLRGNLPYILVGGTKFYERAEIKDL
                     LAYLRLARNPSDGVSFRRVVNVPARGVGAATLEALEAHASEKGLTLLAALHELPSGMT
                     ERAKKALTEFRDLVKRLADFGSAENTGAAAAMAFALEETGLAKLYENSLDPQDEARRE
                     NLDELLAAAREHERSASYGGEGDDPTIAGFLDAVTLRADADEADEKKGILLITLHAAK
                     GLEFDDVFLAGAEDGSLPHASSRDDDDAFEEERRLAYVGMTRAKERLTISFVRRRMVR
                     GEWMSRDRSPFLDAIPGEVVAYEDMTSGFGGRTGDSDFLEGNRGRGYGGRQSFGGQSF
                     GGGRASGGAGAGGSLFPDYENESQEVGALPSSPSKKTLFGRPPMRSLSPVRPVMKRTP
                     PPPTASGYRRGSKVLHPEYGVGVVLTVEGSGDAEKLVVYFDRAGRKKFVAKFANLSPA
                     "
     gene            4075..5553
                     /gene="glpK"
                     /locus_tag="PLB01_07840"
     CDS             4075..5553
                     /gene="glpK"
                     /locus_tag="PLB01_07840"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQQ77252.1"
                     /translation="MSFVLALDQGTTSSRALLFDQGGRVVATAQKEFRQIFPEPGWVE
                     HDPTEIWATQSGVMHEALAKAGIGPRDVAAIGVTNQRETTLLWERATGRPLANAIVWQ
                     DRRTAPICDALRGAGHEPTFTAKTGLVLDAYFSGSKLKWLLDHVPGARARAARGELAF
                     GTVDTWLVWQLTDGAVHATDASNASRTLLYDIHAGAWDDELLRLLDVPREVLPAVVPS
                     SGALATTSLGGVPVPLAGFVGDQQAALFGQLCDRPGLAKCTYGTGCFLLLNTGDSAVA
                     SRNRLLTTVAWDLGAGPTYALEGSVFIAGAAIQWLRDGLKLIRTAADVEALAGSEKDN
                     GGVYFVPAFAGLGAPHWDAYARGAAFGLTRGSTAGHLARAALESIAFQSADVLRAMEK
                     DAGLTLAELRVDGGASANDLLMRFQADLLGVPVVRPAVLETTALGAAYLAGLAVGFWK
                     DAGEIRANVRADRRFEPAMSRDEAAALHAGWERAVSRAKAWA"
     gene            complement(5557..7401)
                     /gene="glmS"
                     /locus_tag="PLB01_07845"
     CDS             complement(5557..7401)
                     /gene="glmS"
                     /locus_tag="PLB01_07845"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQQ77253.1"
                     /translation="MCGIVGYVGPREATPILLDGLKRLEYRGYDSAGIAVVGGDGEVR
                     VARSEGKLGNLTGKLASAPPPGRFGIGHTRWATHGRPSEENAHPHRDASGRIVVIHNG
                     IIENFLPLKQGLQKEGVHFLSETDTEVVAHALAAARRAAPDKPFAEIVRETVSGLTGM
                     YALVLFSADEPGVLYALKWGPPIVLGVGNGENFVASDATALLPHTRDLIFLEDGDLAR
                     ITADAVTVTGFDGIPRSRPASRVPWDAVSAEKGGYPHFMAKEIAEQPTVVIETIGNKL
                     SLETGLFNEEEMGVPKALLREIDRIQIVACGTSWHAGLVAKFLLEEIARVRTDVDYAS
                     EFRYRAPLVDARTLVVGISQSGETADTVAALKEAKALGGRTVGVVNVPGSAIARLVDG
                     VLATHAGPEVGVASTKAFTTQLVALVLFALYVKSVHSGGETPVEAEFLSALARLPSAL
                     RETLALEPAIEKLSARFEKVSHALFLGRGAHYPIALEGALKLKEISYIHAEGYPAGEM
                     KHGPIALIDEAMPIVGIAPQDALYEKTASNLREVKARQGILFALVNPGDADVSTFADE
                     VLVMPKVHPALQPVVSVVPLQLLAYHVARRRGCDVDQPRNLAKSVTVE"
     gene            complement(7406..8818)
                     /gene="glmU"
                     /locus_tag="PLB01_07850"
     CDS             complement(7406..8818)
                     /gene="glmU"
                     /locus_tag="PLB01_07850"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HQQ77254.1"
                     /translation="MTSVVLLAAGRGVRMNSSRPKMLHEAAGRPLLDRALDVALAVAG
                     EAAAVVVVVGKEDSSKERATVASHVRATRPGIRIAIQDPPRGTGDAVRAAAASGAFGK
                     AKTVVVLSGDVPLLSVDAVKSLVDALGKDKRAAVAVLTATLANPTGYGRIVRDKKKSF
                     VRIREEKDSSNKEKGIREINTGTYAFDRAFLESALPRLSSKNSQSEFYLTDVLSLARR
                     AGRRVLPLEGDPSSALGVNSREDLAVVDRLLRERAVAAAMRGGATILRPETVTLDESV
                     VLEPDTTIEPFVTLLGKTHVGAGTVVGQGCVVKNTVLGKNVTVKPYCVIESASVGDGC
                     VVGPFARLREGTDLAAGVHVGNFVETKKAKLHAGVKANHLTYLGDTEIGPRTNVGAGV
                     ITCNYDGFAKHRTTIGADVFVGSDTQLVAPVTVGDGAIIGAGTTVTKDVPRDGLAVSR
                     VPQKNLEGGGAAYRARKKKS"
     gene            complement(8815..9498)
                     /locus_tag="PLB01_07855"
     CDS             complement(8815..9498)
                     /locus_tag="PLB01_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77255.1"
                     /translation="MTSKSKWTEDDARWLKANFGRMDSQTLSRKLGLSLEDLEKKVRQ
                     LKLVAHEPDKTRKPPASLKEAVRETSAARKEYEKAIDLFHKRHFDEAARKFEDLIVKH
                     PDEKEFLDRARMYLTACRNGKKGKASAGDSPEEIYHAAVFEKNRGNVEKALELLKKNA
                     GKKDGDGRSHYLAACCFALQGDVEQALQALRKAIAADDQNRIQARLEADLSVLRGTQG
                     FTELVAGGV"
     gene            9659..10171
                     /locus_tag="PLB01_07860"
     CDS             9659..10171
                     /locus_tag="PLB01_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQQ77256.1"
                     /translation="MYELPIFCTQCARKLESKEMGPGRSVPVCPACGAVHWIDPKVAA
                     GVLIVRDERVLLVKRAIEPGYGKWTFPGGHVDRGETVEEAALRETLEECGAIADLEGL
                     LGLFSYPGRPVVVAVYRGLLAPGSREPHAGDETLEVGWFTPEEVAGLELAFQSVADAL
                     TKLFLRPYVL"
     gene            10241..10882
                     /gene="msrA"
                     /locus_tag="PLB01_07865"
     CDS             10241..10882
                     /gene="msrA"
                     /locus_tag="PLB01_07865"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQQ77257.1"
                     /translation="MRRNTRFVLPLFALLVFAAFARADEPTQATPPAPQGLAKATFAG
                     GCFWCMQGPYDAVPGVVSTTVGYTGGKKAGVTYHEVGAGGTGHREAIEVVYDPKKVSY
                     EKLLDVFWHNVDPTYAGGQFCDSGFQYTTAIFVHDDAQRAAAEASRSALEASKVLKAP
                     IVTEIVKAGVFWKAEEYHQSYYKKNPIRYNFYRSGCGRDSRLKQLWGDKAGSH"
     gene            complement(10887..11678)
                     /locus_tag="PLB01_07870"
     CDS             complement(10887..11678)
                     /locus_tag="PLB01_07870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ77258.1"
                     /translation="MRDAGLAVVTGASSGIGAAFARLLASQKRPVLLVSRSGERLEAV
                     AAGLNAGPGGGAEVLAADLATPVGRDALWNATEGAGRPVALLVNNAGFGLNGPETDLP
                     FERVQALLELNVVATAELTHRFLVAMKARGAGAILNVASTSAFYPTPYFSLYGASKAF
                     ILSFTEALHEEALSFGVTVTCLCPGFTRTNFAAVAGMKEGKETPFPEMAPEAVARIGL
                     EAVRKRKAVVVTHPLDRAWIAAGRLVPRWVPAKLGVRFFKKTKIS"
     gene            complement(11675..12580)
                     /locus_tag="PLB01_07875"
     CDS             complement(11675..12580)
                     /locus_tag="PLB01_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77259.1"
                     /translation="MRFPRRAVPALFAAAAAAAVPACGKAPVRDGAVTPAPPAVLEGG
                     IPLVPGLLLTLLPNLPGVDGEDVPDLARREVEVLEAENGRLRLKWTGQVRVEKAESAR
                     RREDWARARANAPRGATPEPAVPAVYETREVGGTLSFPDFGTATELLLPGLWPEGNAT
                     IPGSTALWISANARAELKGGGSASVPLAASALSLREPGATHLRRAIDLSAGASGRPGV
                     WTAGPPVSFALRVDGEDVRVPALAARDWFATYAVLDGSSPALVLAALPQLPSSPPADL
                     FAPAHAMRSLLGYRVAEVTRPKEKK"
     gene            complement(12585..13364)
                     /locus_tag="PLB01_07880"
     CDS             complement(12585..13364)
                     /locus_tag="PLB01_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HQQ77260.1"
                     /translation="MSLALMGASCETVRPNVKAQSDVPSAGKFPHGIFGRFLEKVVHD
                     GLVDYATASLEESGLEEYLAEVARVSPESHPHLFPTEPDRLAYWINAYNACAIRGVLR
                     FNRPQSLKEIAHRFDSETKYVFGGRELSLNQISSLAWKRFTDARIHFALVKARRGGPP
                     LAKDAWDAADLEDRLEAAAKAFVADSRNVDWKAPSLKAGLSRVILDFRGDFEREVPST
                     VSGDPRLIGSINRWRPRLEKVTATSVYPIPLDERLNDVSNR"
     gene            complement(13414..14211)
                     /locus_tag="PLB01_07885"
     CDS             complement(13414..14211)
                     /locus_tag="PLB01_07885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ77261.1"
                     /translation="MANRSWALVLGASSGFGEGTALALARAGRNVFGVHLDRKTTQPN
                     VERITSEITAAGGRPVFFNMNAADDEKRAEAIAEMTKILDAEGRRAGIDVVLHSLAFG
                     TLKPFLMDSPADRINRAQMDMTLDVMAHSLVYWVQDLLAAGLLAEGGHVFAMTSSGGH
                     RIWKSYGAVSAAKAALESHCRQLAVELVTRKIAVNAIRAGVTDTPALRKIPDNAAMIE
                     RCLAIHPAGRLTTPDDVARVIVALANPGTVWLSGNVLGVDGTEDIIG"
     gene            14265..14945
                     /locus_tag="PLB01_07890"
     CDS             14265..14945
                     /locus_tag="PLB01_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQQ77262.1"
                     /translation="MHVRTAADFRRRLREGPPLVLDAAMGTDLLARGVPLPAPLWSAQ
                     ALVDAPDLVEAIHRENAEAGANVLTIASFRLHGRNLRGSSCSLSQRALLSLAVDTARR
                     ATKTGISSKGEERAQQGRPAGRPSEPFTPPSKKIFDSPWIAGSLAPLEDCYRPDRVPS
                     EAEISSEHAEMAAALAAAGVDLVLVETMNTVREALAAARGAAQMTRSVDSGPSAAKAR
                     ARAASGGA"
BASE COUNT         2119 a         5387 c         5235 g         2256 t
ORIGIN      
        1 cgtcgtcctc cgcgccccgt tctccaccgc gaaacaggtc gagagcctgc acgaggccct
       61 gtgcttcctg cgcgcctggc ccgacgacgc ggccgtcctc gcgcggaccg agtttctcct
      121 cgcgcgcttc gcccggcggc ccgacctgaa acgccaccgc agcgcgctgg aggacagcgg
      181 cattgcgggc acgaggacgc gcttcccgtt cttcgccggc accgcgcgct ggctcgccgc
      241 gcgctggggc gggaagctcc gcgtcgactg gaaggcgttc gagaagggag ccgctctcga
      301 gcgcctcctc cctctcgtct ccctctgggc cgagacgccc gcgctcgacg agctcgacct
      361 cgggacgcgc ggctgggtga accgcctcaa aggccctcgc gaaaccgacg ccgccttcct
      421 cgtccggagg cttgcgacgg tcggcagggg gacgttcgag aaggagtggg cctgggaggg
      481 cctcgagctg ccgctcgtcc tcgagcccgg ccccggtacg ccctcgcgga cgaacgcgtg
      541 cctccggacg gcgcccgtcg tcttccagac gcggccgctc gaccggggcc gccccgacgt
      601 ccggagggag ctcgcccggc cgccgctctc ggtcgcggcc gtcccgcgcc gggaggcgga
      661 gcgttacctc gacctcgcgc gggaagcgat ggtcgcgcgc gcgcgggacc tcgacgcctt
      721 ccagtggggc gacccgcagg acgtccgcct cgtgacgtac ccggacggcc tcgcgttcgc
      781 cgcgatcggc gtcgtccccg agcggcggct cctcctcgaa tccgtctacg ggttcctgac
      841 gctcaagaac ggcgtcccga tcggctacgt cctcacgagc gccctcttcg gctcctccga
      901 gatcgcctac aacgtcttcg agtcgttccg cggcggcgag tcgggcctcg tctacggcaa
      961 ggtcctctcg atgacgcgcg ccctgttcgg gtcggacgtc ttcacgatct ttccgtatca
     1021 gctcggcggc tacggcaacc ccgaggcgct ccagtcgggc gcgtggtggt tctatcgcaa
     1081 gctcggcttc gcgccccgcg ccccgcgcgc cgtggcgctc atgaacgagg aagagcggcg
     1141 gatggccgcg gacccgaagc accgaacctc cgtcccggac ctcaaggtcc tcgcgtcgga
     1201 gaatctctac tggttcggcg ggagggtgcg cagcgacgcg atcggcctcg tggagctccc
     1261 gaacgtcggc ctccgcgtcg tcgacgccgt gcgatcccga ttcggctctg accgggagcg
     1321 agccgaggcg gtcctgacag cggaggcggc ccgccttctc ggtgcgcgga cggccgcgtg
     1381 gtcgcccgcc gagcgcctcg ccttccggcg ctgggcgccg ctcgtctcgg tcctgccggg
     1441 cgtcgaggcc tggtcgcccg aagagaaacg cgcgctcgcc gccgtgatcc gcgccaaggg
     1501 cggccggcgc gagtcggact tcgtccgcct tttcgacgcc caccggaagc tccggaaagc
     1561 ggtcgtcgag ctggcgaagg atcccggcta ggccggcgag aggttcgcga acttcgcgac
     1621 gaacttcttg cggcccgcgc ggtcgaaata gacgaccagc ttctcggcgt cgccggagcc
     1681 ctcgaccgtc aggaccacac ccacgccgta ctcggggtgc agcaccttcg acccgcggcg
     1741 gtagccggac gcggtcggcg gcggcggcgt ccgcttcatg accggcctga cggggctcag
     1801 gctgcgcatc gggggacgtc cgaagagagt tttcttagaa ggtgaagagg ggagggcgcc
     1861 gacctcctgg gattcgttct cgtagtcggg aaacagcgag ccgcccgccc ctgcgccgcc
     1921 cgaggcgcgc cccccgccga acgactgccc accgaaggac tgccgcccgc cgtagccccg
     1981 gcccctatta ccttctaaga aatccgaatc tccggttctt cctccgaaac cagacgtcat
     2041 gtcttcgtac gcgacgacct cccccgggat tgcgtcgagg aagggagacc ggtcgcggct
     2101 catccactcg ccgcgcacca tccggcggcg gacgaaggag atcgtgaggc gctccttggc
     2161 gcgcgtcatc ccgacgtacg cgaggcgccg ctcctcctcg aacgcgtcgt cgtcgtcgcg
     2221 cgaggaggcg tgcgggagcg agccgtcctc ggcgcccgcg aggaagacgt cgtcgaactc
     2281 gaggcccttg gccgcgtgga gcgtgatgag gaggatcccc ttcttctcgt cggcctcgtc
     2341 ggcgtcggcg cgcagcgtca ccgcgtcgag gaagccggcg atcgtggggt cgtcgccctc
     2401 gccgccgtac gacgccgagc gctcgtgctc gcgcgccgcc gcgaggagct cgtcgaggtt
     2461 ctcgcggcgg gcctcgtcct gcgggtcgag ggagttctcg tagagcttcg cgaggccggt
     2521 ctcctcgagc gcgaacgcca tcgcggccgc cgcgcccgtg ttctcggccg agccgaagtc
     2581 ggcgaggcgc ttcacgaggt cccggaactc cgtcagggcc tttttcgccc tctccgtcat
     2641 gccggagggc agctcgtgga gggccgcgag cagggtgagg cccttctccg acgcgtgcgc
     2701 ctccagcgcc tccagcgtcg cggcgcccac gccgcgcgcc ggcacgttga ccacccggcg
     2761 gaacgacacg ccgtccgaag ggttccgcgc gagacgcagg tacgcgagga ggtccttgat
     2821 ctcggcccgc tcgtagaact tcgtcccgcc gacgaggatg tacggcaggt tcccgcgcag
     2881 gagctcgtcc tcgaacggcc gggactgggc gttcgtccgg aagaggaccg ccacctcgcc
     2941 ctgcggccgg gcgcggcggg ccgcggcgac gcgcgcgacg atctcgcggg cctcgtcgcg
     3001 ctcctcgtcg aacgcgacga gccggacctt ctcgccgccc gtcttcgtcg ccttcagcgt
     3061 cttgccgagc cggcgcctgt tctcggcgac gaccgcgccc gccgccttga ggatcggcgc
     3121 cgtcgagcgg tagttgcgct cgaggcggac cgtcttcgcc gtcgggaagt cgcgcgtgaa
     3181 ctcgaggatg ttcgagacgt ccgccccgcg ccagcggtag atggactggt cctcgtcgcc
     3241 cacgacgaag aggttcccgg cctcgcccgt cacgagcttc acgagcttgg cctgcacgcg
     3301 gttcgtgtcc tggtactcgt cgacgagcag atgacgaatg ctccgccgga ggagtgcccg
     3361 cacggcgtcg ttcgtcgcga cgagcttcgc cgaaaggacg aggaggtcgt cgaaatccag
     3421 cgcttgggac gcccggagcg tcttctcgta ccggcgcgcc acgtccgcga ggcgcgagcc
     3481 gaggaagtct ccgaaccgcc gcgggaagtc gtccggcgag atgccctcgt tcttggccgc
     3541 ggagatcttc gactggatct gccggggcgt cgcggacttc tccgggatcg cgacctcctt
     3601 catgacgcgc ttgacgacgg cgagctggtc gtccgagtcg aagacgacga acccgcgcgg
     3661 gagccccgcc tcgtcggcga agcggcgcag caggcgcacg ccccaggagt ggaacgtccc
     3721 gacgtacgag cgcgggcgct cggtgccgag gaggttcgcg acgcgctctt tcatctcgcc
     3781 ggcagccttg ttcgtgaacg tgaccgcgac gatgccttcc tgcgggacgc cgtgcgcgac
     3841 gagccacgcg aggcggcgcg tcaggacgcg cgtcttcccc gagccggctc ccgcgagaac
     3901 gagcagcggc ccttcgccgt gcgagacggc ggcggcctgt tcgggattga ggccttcgag
     3961 gagatcttcc gggcgggcga ccaaggggcg cgatggtagc gcagagcgcc gacccgatgc
     4021 agtcgcatcc tcccccgctg acggcagggc cctgcggcag aatcggccgc accgatgtcc
     4081 ttcgtcctcg ccctcgacca gggcacgacc agctcgcgcg ccctcctctt cgaccaggga
     4141 gggcgcgtcg tcgcgaccgc gcagaaggag ttccgacaga tctttccgga gcccggatgg
     4201 gtggagcacg acccgacgga gatctgggcg acgcagtcgg gggtgatgca cgaggcgctc
     4261 gcgaaggccg gaatcggccc ccgcgacgtc gccgcgatcg gggtcacgaa ccagcgcgag
     4321 acgacgctcc tctgggagcg cgcgacgggc cgcccgctcg cgaacgcgat cgtctggcag
     4381 gaccggcgga ccgcgccgat ctgcgacgcg ctgcgggggg ccggccacga gccgacgttc
     4441 accgcgaaga ccggcctcgt cctcgacgcg tacttctccg ggtcgaagct gaagtggctc
     4501 ctcgaccacg ttcccggcgc ccgcgcgcgg gccgcgcgcg gggagctcgc gttcgggacc
     4561 gtcgacacgt ggctcgtctg gcagctcacg gacggcgccg tgcacgcgac ggacgcctcc
     4621 aacgcgagcc ggacccttct ctacgacatc cacgcgggcg cgtgggacga cgagctcctc
     4681 cggctcctcg acgtgccccg cgaggtgctt cccgcggtcg tgccctcctc gggcgccctc
     4741 gcgacgacgt ccctcggagg tgttcccgtc cccctcgcgg gcttcgtggg cgaccagcag
     4801 gcggcgctct tcggacagct ctgcgaccgt ccgggtctcg cgaagtgcac gtacggcacc
     4861 gggtgtttcc tgctcctcaa cacgggcgac tccgccgtcg cctcgcgaaa ccgtctcctg
     4921 acgaccgtcg cctgggacct cggcgcgggg ccgacgtacg ctctcgaggg cagtgtcttc
     4981 atcgcgggtg ccgcgatcca gtggctgcgg gacggcctga agctgatccg gaccgccgcc
     5041 gacgtcgagg cgctcgccgg gagcgagaaa gacaacggcg gcgtgtactt cgtgccggct
     5101 ttcgcggggc tcggggcgcc gcactgggac gcctacgccc gcggcgccgc cttcggcctc
     5161 acgcgcggct ccacggccgg ccacctcgcg cgcgccgccc tcgagtcgat cgcgttccag
     5221 agcgcggacg tcctccgggc gatggagaag gacgcggggc tcacgctcgc cgagctgcgc
     5281 gtggacggcg gcgcctcggc gaacgacctc ctcatgcggt tccaggcgga cctcctcggc
     5341 gtccccgtcg tgcggccggc ggtcctcgag acgacggccc tcggcgccgc gtacctcgcg
     5401 ggcctcgccg tcggcttctg gaaggacgcg ggcgagatcc gcgcgaacgt gcgcgcggac
     5461 cgccgcttcg agcccgcgat gtcgcgcgac gaggccgccg cgctccacgc cgggtgggag
     5521 agagcggtca gccgagcgaa ggcctgggcc tgatcctcat tccaccgtca cgctcttcgc
     5581 gaggttgcgg ggctggtcca cgtcgcagcc gcggcggcgg gcgacgtggt acgcgaggag
     5641 ctggagcggg accacggaga cgacgggctg gagcgccggg tggaccttcg gcatcacgag
     5701 gacctcgtcc gcgaacgtcg agacgtccgc gtcgccgggg ttcacgagcg cgaacaggat
     5761 cccctgccgc gccttgactt cccggaggtt cgacgccgtc ttctcgtaga gcgcgtcctg
     5821 gggcgcgatt ccgacgatgg gcatggcctc gtcgatgagc gcgatcggcc cgtgcttcat
     5881 ctctccggcg ggatagccct cggcgtggat gtacgagatc tccttgagct tgagcgcgcc
     5941 ctcgagcgcg atcgggtagt gcgcgccgcg cccgaggaag agcgcgtggg agaccttctc
     6001 gaaccgcgcc gacagcttct cgatcgcggg ctcgagcgcg agcgtctcgc ggagcgccga
     6061 gggaaggcgc gcgagggccg aaaggaattc cgcctccacg ggcgtctcgc cgccggaatg
     6121 gaccgacttc acgtagagcg cgaagaggac gagcgcgacg agctgcgtcg tgaacgcctt
     6181 cgtcgaggcg acgcccacct ccggccccgc gtgcgtcgcg aggacgccgt ccacgaggcg
     6241 cgcgatcgcc gagccgggca cgttcacgac gcccaccgtc cggccgccga gcgccttcgc
     6301 ctccttgagc gccgcgaccg tgtcggccgt ctcgcccgac tgcgagatcc cgacgacgag
     6361 cgtccgcgcg tccacgagcg gcgcgcggta ccggaactcg gacgcgtagt ccacgtccgt
     6421 cctgacgcgc gcgatctcct ccagcaggaa cttcgcgacg aggcccgcgt gccagctcgt
     6481 cccgcacgcg acgatctgga tgcggtcgat ctcgcggagc agcgccttcg ggacgcccat
     6541 ctcctcctcg ttgaagaggc ccgtctcgag cgagagcttg ttgccgatcg tctcgatcac
     6601 gaccgtcggc tgctcggcga tctccttggc catgaagtgc gggtagccgc ccttctcggc
     6661 cgagacggcg tcccacggca cgcggctcgc ggggcgagat cgcggaatgc cgtcgaaacc
     6721 ggtcaccgtg acggcgtccg ccgtgatccg cgcgaggtcc ccgtcctcga ggaagatcag
     6781 gtcgcgcgtg tgtgggagga gcgccgtcgc gtccgaggcg acgaagttct cgccgttccc
     6841 gacgccgagg acgatcggcg gtccccactt gagggcgtag aggactcccg gctcgtcggc
     6901 cgagaagagg acgagggcgt acatccccgt gaggcccgac acggtctcgc gcacgatttc
     6961 ggcgaacggc ttgtccggcg cggcccggcg cgccgccgcg agcgcgtggg cgacgacttc
     7021 ggtgtccgtc tccgagagga aatggacgcc ctccttctgc aggccctgct tgagcgggag
     7081 gaagttctcg atgatgccgt tgtggatgac gacgatccgg ccgctcgcgt cgcggtgggg
     7141 gtgggcgttc tcctcggacg ggcggccgtg cgtcgcccag cgcgtgtgcc cgatgccgaa
     7201 ccgtcccggg ggcggcgcgc tcgcgagctt cccggtcagg ttcccgagct tgccctcgct
     7261 ccgggccacg cggacctcgc cgtcgcctcc cacgaccgcg atgccggccg agtcgtagcc
     7321 gcggtactcg aggcgcttca acccgtcgag caggatcggc gttgcctcgc gcgggccgac
     7381 gtagcccacg atgccgcaca tcgcgtcagc tcttcttctt gcgggcccgg tacgccgcgc
     7441 cgccgccctc gaggttcttc tgcgggacgc gggagacggc aaggccgtcc ctcgggacgt
     7501 ccttcgtgac cgtcgtgccg gcgccgatga tcgcgccgtc tcccaccgtg accggcgcga
     7561 cgagctgcgt gtccgagccg acgaagacgt cggcgccgat cgtcgtccgg tgcttggcga
     7621 aaccgtcgta attgcacgtg atgacgccag cgcccacgtt cgtgcgcggg ccgatctccg
     7681 tgtcgccgag gtacgtcagg tggttcgcct tgacgcccgc gtggagcttc gccttcttcg
     7741 tctcgacgaa gttgcccacg tggacgcccg cggcgaggtc cgtcccctcg cgcaggcgcg
     7801 cgaacggacc gaccacgcat ccgtctccca cggaggccga ctcgatgacg cagtacggct
     7861 tcacggtgac gttctttccg agaacggtgt tcttcacgac gcagccctgg ccgacgaccg
     7921 ttccggcgcc gacgtgcgtc tttccgagga gcgtcacgaa cggctcgatc gtcgtgtccg
     7981 gctcgaggac gacggactcg tcgagcgtga ccgtctcggg tcggaggatc gtcgccccgc
     8041 ccctcatcgc ggcggcgacg gcgcgctcgc gcaggaggcg gtccacgacc gcgaggtcct
     8101 cgcgggagtt gactccgagc gcggaagaag gatcgccttc gagggggaga acgcggcgac
     8161 cggccctccg cgcgagcgag agcacgtcgg tgagatagaa ctcggattgg gagttcttag
     8221 aagatagacg gggcaacgcg ctctcgagga acgcccgatc gaacgcatag gtcccggtat
     8281 tgatctcccg gatccccttc tccttattcg aagaatcctt ctcctctctt atccgaacaa
     8341 acgacttctt cttgtcccgc acgatgcggc cgtagcccgt cgggttcgcg agcgtcgccg
     8401 tcaggacggc caccgcggcc ctcttgtcct tcccgagcgc gtcgacgagg ctcttcaccg
     8461 cgtccaccga gaggagcggc acgtcgcccg agaggacgac gaccgtcttc gccttcccga
     8521 acgcgcccga tgcggccgcg gcgcgaaccg cgtcgccggt tccccgtggc ggatcctgta
     8581 tcgcgatccg gatgccggga cgggtcgccc tcacgtgaga cgcgaccgtc gctctttcct
     8641 tcgaagaatc ttctttcccg accacaacga cgacggccgc cgcctcgccc gcgacggcga
     8701 gcgcgacgtc cagcgcgcgg tcgagcagcg gccggcccgc cgcctcgtga agcattttcg
     8761 gccgcgacga attcatcctc acgccgcggc cggcggccag gaggacgacg gaggtcacac
     8821 gcctcccgcg acgagctccg tgaacccctg cgtgccgcgc agaaccgaca ggtccgcctc
     8881 gagccgcgcc tggatgcgat tctggtcgtc ggccgcgatc gccttgcgga gcgcctggag
     8941 cgcctgctcg acgtccccct gcagcgcgaa gcagcacgcc gcgaggtagt ggctgcgccc
     9001 gtctccgtcc ttctttcccg cgttcttctt gagcagctcg agggccttct cgacgtttcc
     9061 acggttcttc tcgaacacgg cggcgtggta gatctcctcg gggctgtccc cggcggaggc
     9121 cttgcccttc ttcccgttcc ggcacgccgt caggtacatg cgcgcgcggt cgaggaattc
     9181 cttctcgtcg gggtgcttca cgatgaggtc ttcgaacttc cgggccgcct cgtcgaagtg
     9241 ccgcttgtgg aagaggtcga tggccttctc gtactccttg cgcgccgccg aagtctcgcg
     9301 caccgcttcc ttgagcgacg cgggcggctt gcgcgtcttg tcgggctcgt gcgcgacgag
     9361 cttgagctgc ctgaccttct tctcgaggtc ctcgagcgag aggcccagct tgcgcgagag
     9421 cgtctgggag tccatgcgcc cgaagttggc cttgagccac cgggcgtcgt cttcggtcca
     9481 cttgcttttg ctggtcaagg gccctccggg gaagcgggcc cgaggcccgc aaggacggtg
     9541 gattctcgtt acgtcctcgt catccgtcaa ggacgtcgaa aaacagccga aagcccctcc
     9601 aaggtagggc cgggcgcccc gcctgtcacg gccgccgaaa cgcggtagcc tgaaggcggt
     9661 gtacgaactc cccatcttct gcacgcagtg cgcgcggaag ctcgaatcga aggaaatggg
     9721 cccggggcgc agcgtgcccg tgtgccccgc ctgcggcgcc gtgcactgga tcgacccgaa
     9781 ggtcgcggcg ggagtcctga tcgtccgcga cgagcgcgtc ctcctcgtga agcgcgcgat
     9841 cgagcccggc tacggcaagt ggacgtttcc gggcggccac gtggaccggg gggagaccgt
     9901 cgaggaggcg gccctgcgcg agacgctcga ggagtgcggc gcgatcgcgg acctggaagg
     9961 gcttctcggc ctcttttcgt atccgggccg gcccgtcgtg gtggcggtct accgcgggct
    10021 cctcgccccc ggcagccgcg agccgcacgc cggcgacgag acgctcgagg tcggttggtt
    10081 cacgcccgag gaagtcgccg gcctcgagct cgcctttcag tcggtggccg acgcgctcac
    10141 gaaactcttc cttcgtccgt acgttctctg actgggaatg agacggcccc cctccggcat
    10201 ccacggaggg gaggccccgc gtatcccctc cggagaccgc atgcgccgca acacccgatt
    10261 cgtccttccc ctcttcgctc tcctcgtctt cgcggccttc gcccgcgcgg acgagcccac
    10321 gcaggccacg ccgcccgcgc cccaaggcct cgcgaaagcg acgtttgcgg ggggctgctt
    10381 ctggtgcatg caggggccct acgacgccgt cccgggcgtc gtctcgacga ccgtggggta
    10441 tacgggcggc aagaaggccg gcgtgacgta ccacgaggtc ggcgcgggcg gcacggggca
    10501 ccgcgaggcg atcgaggtcg tctacgaccc gaagaaggtc tcgtacgaga agctcctcga
    10561 cgtcttctgg cacaacgtcg accccacgta cgcgggcggc cagttctgcg attccggctt
    10621 ccagtacacg acggcgatct tcgtccacga cgacgcgcag cgggccgcgg ccgaggcgtc
    10681 gcgcagcgcg ctcgaggctt cgaaggtcct caaggcgccg atcgtgacgg agatcgtgaa
    10741 ggcgggagtc ttctggaagg ccgaggagta ccaccagagc tactacaaga agaacccgat
    10801 ccggtacaac ttctaccgct cgggctgcgg ccgcgattca cggctcaagc agctctgggg
    10861 ggacaaggcc gggagccact gaccttctaa gagatcttcg tcttcttgaa gaaccgcacg
    10921 ccgagcttcg cgggcaccca gcgggggacg agcctgcccg cggcgatcca ggcgcggtcg
    10981 agcgggtgcg tcacgacgac cgccttacgt ttccgcacgg cttcgaggcc gatcctcgcg
    11041 accgcctcgg gcgccatctc gggaaacggc gtctctttcc cctccttcat ccccgcgacc
    11101 gcggcaaaat tcgtcctcgt gaagcccggg cagaggcacg tgacggtcac gccgaacgag
    11161 agcgcctctt cgtgcagcgc ctccgtgaag ctcaggatga aggccttgct cgcgccgtag
    11221 agcgagaagt acggcgtcgg gtagaaggcg gacgtcgagg cgacgttcag gatcgcgccc
    11281 gcgccgcgcg ccttcatcgc gacgaggaac cggtgcgtga gctccgccgt cgcgacgacg
    11341 ttcagttcga ggagcgcttg caccctctcg aacggcaggt ccgtctcggg cccgttcagg
    11401 ccgaaacccg cgttgttcac gagcagcgcg accggccgcc ccgcgccctc cgtcgcgttc
    11461 cagagcgcgt cgcgccccac gggcgtcgcg agatcggccg cgaggacctc cgccccgccg
    11521 cccgggccgg cgttcagccc ggccgcaacg gcctcgagcc tttcccccga gcgcgagacg
    11581 agcaggaccg gccgcttctg cgacgcgagg aggcgggcga acgcggcgcc gatccccgac
    11641 gacgcgccgg tgacgacggc gaggccggcg tccctcactt cttctccttc gggcgcgtca
    11701 cctcggcgac gcggtagccg agcaggctcc gcatcgcgtg cgcgggagcg aagagatccg
    11761 cgggcggcga agacggcagc tgcggcagcg ccgcgagcac gagtgcgggg gagctcccgt
    11821 cgagaacggc gtaggtcgcg aaccagtcac gcgccgcgag cgcgggaacc ctgacgtcct
    11881 ccccgtcgac acgcagggcg aaggaaacgg gcggcccggc cgtccagacg ccggggcggc
    11941 ccgacgcgcc cgcggagagg tcgatcgcgc gccgcagatg cgtcgcaccg ggttcgcgca
    12001 gcgagagcgc cgacgccgcg agcggcaccg aggcgctccc gccgcccttg agctcggcgc
    12061 gggcgttcgc ggagatccag agcgccgtcg accccggaat cgtggcattg ccctccggcc
    12121 agaggcccgg aaggagcagc tccgtcgccg tcccgaaatc cggaaaggac agagtgcccc
    12181 cgacctcccg cgtctcgtag acggccggaa ccgccggctc cggcgtcgcg cctcgcggcg
    12241 cgttcgcgcg cgcgcgcgcc cagtcttcgc ggcggcgcgc agactcggct ttctccacgc
    12301 gcacctgccc cgtccacttc aggcggagcc ttccgttctc ggcttcgagg acctccactt
    12361 cgcggcgggc gaggtccggg acgtcctcgc cgtcgacgcc cggcaggttc ggcagcagcg
    12421 tcaggaggag gccgggcacc aaagggatgc cgccctcgag gacggcgggg ggcgcgggcg
    12481 ttacggcccc gtcgcgcacg ggcgccttcc cgcacgcggg gacggcggcg gcggccgccg
    12541 ccgcgaacag ggccgggacg gcgcgacgcg ggaagcgcat gggatcagcg gttcgagacg
    12601 tcgttcaggc gttcgtcgag cgggatcgga tagaccgacg tcgccgtgac cttttccagg
    12661 cgcggccgcc accggttgat cgagccgatg agacgcgggt cccccgacac ggtcgacggg
    12721 acctcgcgct cgaagtctcc gcggaagtcg aggatcacgc gcgagagccc ggccttgagg
    12781 gacggcgcct tccagtcgac gttgcgcgag tccgcgacga acgccttcgc ggcggcctcg
    12841 aggcggtcct cgaggtcggc cgcgtcccag gcgtccttcg cgagcggagg gccgccgcgg
    12901 cgcgccttca cgagggcgaa gtggatgcgt gcgtccgtga agcgcttcca cgcgagcgac
    12961 gaaatctggt tcagggagag ctcgcgccct ccgaacacgt acttcgtctc ggagtcgaac
    13021 ctgtgcgcga tctccttcag gctctggggg cggttgaagc gaaggacgcc gcggatcgcg
    13081 cacgcgttgt acgcgttgat ccagtacgcg aggcggtccg gctccgtcgg gaagaggtgc
    13141 gggtgcgact ccggcgagac gcgcgcgacc tcggcgaggt actcctcgag gccggactcc
    13201 tcgagggacg ccgtcgcgta gtccacgagc ccgtcgtgga cgaccttctc gaggaagcgt
    13261 ccgaagatcc cgtgcgggaa cttcccggcc gacgggacgt ccgactgcgc cttcacgttc
    13321 gggcgcaccg tctcgcagga cgcgcccatc agcgcgagcg acaacgccgc gcaagccgaa
    13381 gccgacaaga agcgcgcttc cctcatcgcg tcgtcagccg atgatgtctt ccgtgccgtc
    13441 gaccccgagc acgttgccgg aaagccagac ggtcccgggg ttcgcgagcg cgacgatcac
    13501 gcgtgcgacg tcgtcgggcg tcgtgagacg gcccgccggg tggatcgcga gacagcgttc
    13561 gatcatcgcg gcgttgtcgg gaatcttccg cagggccggc gtgtccgtga cgcccgcgcg
    13621 gatcgcgttc accgcgatct tccgggtcac gagttccacc gcgagctggc ggcagtggct
    13681 ctcgagcgcg gccttcgcgg ccgaaaccgc gccgtaggat ttccagatcc ggtggccgcc
    13741 ggaagacgtc attgcgaaga cgtgcccccc ttccgcaagg agacccgcgg caaggaggtc
    13801 ctggacccag tagacgagcg aatgcgccat gacgtcgagg gtcatgtcca tctgggcgcg
    13861 gttgatccgg tcggcggggc tgtccatcag gaacggcttg agggtcccga acgcgagcga
    13921 atggaggacg acgtcgatcc cggcgcggcg gccctcggcg tcgaggatct tcgtcatctc
    13981 cgcgattgcc tcggccctct tctcgtcgtc ggcagcgttc atgttgaaga agacgggccg
    14041 cccgccggcc gccgtgatct ccgacgtgat ccgctcgacg ttgggctggg tggtcttgcg
    14101 gtcgaggtgg acgccgaaca cgttccgacc cgcccgggcg agggcgagag ccgtgccttc
    14161 cccgaatccg gaggaggccc cgaggacgag ggcccacgaa cggtttgcca tggcccggat
    14221 gctagcaggc ccgccccgct atcctcgtct ccggggaggc cgccgtgcac gtccggaccg
    14281 cagcggattt ccgccggaga ctgcgcgagg ggccgccgct cgtcctcgac gcggcgatgg
    14341 gcacggacct gctcgcccgc ggcgtccccc tgccggcgcc tctctggagc gcgcaggcgc
    14401 tcgtcgacgc gccggacctc gtcgaggcga tccaccggga aaacgccgag gcgggggcga
    14461 acgtgctcac gatcgcgagc ttccggctgc acgggaggaa tctaagagga tcttcttgct
    14521 ccctctcaca acgagccctg ctctccctcg ccgtagacac agcgcgaagg gcgacgaaaa
    14581 ctgggatttc ttcgaaaggt gaagaacggg cgcagcaggg gcgcccggcc gggcgcccga
    14641 gcgagccttt cactccacct tccaagaaga tcttcgacag cccctggata gccggctcgc
    14701 tggcacccct ggaggattgc taccgtcctg atcgggtccc ctccgaagca gaaatctctt
    14761 ccgagcacgc ggagatggcg gcggctctcg ccgccgcggg cgtggacctc gtcctcgtcg
    14821 agacgatgaa cacggtccgc gaggccttgg ccgccgcgcg aggcgcggcg cagatgacga
    14881 gatcggttga cagcggaccc tcagcggcga aggcccgcgc tcgtgcggcc agcggaggcg
    14941 catgagcacc ggcgcccccc caacctacga gcaagccctc gccacgctca ccgcgcc
//
LOCUS       DAOMFY010000072        14939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1234_length_14939_cov_6.989519, whole genome shotgun sequence.
ACCESSION   DAOMFY010000072 DAOMFY010000000
VERSION     DAOMFY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_14939_cov_6.989519"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLB01_07895"
     CDS             <1..854
                     /locus_tag="PLB01_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ77263.1"
                     /translation="GATAGQGVVWYTGQFYALFFLTITLKFDWATAYLLIALSLVIGT
                     PFFIVFGALSDKIGRLKIILTGCAIAAITYIPLFKALTHYGNPALEKYQNSTPISIAG
                     TNCQMHIFVTPFTKFSACDKAQDFMSKNGLSFATLPPQADDVVIKVGSIEIKGYDEAK
                     LKEAFKQTGYPNPAFKKDEMNFGMVLLILVIMVLYVTMVYGPIAAFLVELFPANIRYT
                     SMSLPYHIGNGWFGGMLPLIATAWVAASGNIYQGLWYPIIVSVITVVIGGLFLRETKD
                     VKIHEEV"
     gene            964..2166
                     /gene="nhaA"
                     /locus_tag="PLB01_07900"
     CDS             964..2166
                     /gene="nhaA"
                     /locus_tag="PLB01_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQQ77264.1"
                     /translation="MPRREPIAPLAFLQQFMRTEAASGVLLLAAAVAAIVFANSPLQS
                     VYAHATHLPLRFGDGPWRVDTTLLHAVNDGLMAVFFLVVGLEIKREFLTGELSGKGAA
                     ILPVAAACGGAVIPASIYAAFNAGTPAAQGWGIPMATDIAFAVGVLALLGRHVAPVFK
                     VFLTAVAVVDDLIAVLVIAFFYTSGLDLRALAVVAGACVLLYALNRSGVQALAPYLLV
                     GLVLWAAMAKSGVHATIAGVLLGMSIPAAGAETSPLRRLEHALHPWVAWGIMPVFALA
                     NAGVTVPAGKLGAMLTSPIALGIVLGLFVGKQVGIFGTTFLLARLGVGAIPLDARARR
                     SLWGVALLGGIGFTMSLFVSGLAFTDEAMADVSKAAILAGSLISGLAGAAVLASVPEP
                     VAGDADAG"
     gene            2182..2586
                     /locus_tag="PLB01_07905"
     CDS             2182..2586
                     /locus_tag="PLB01_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQQ77265.1"
                     /translation="MAIFGKSSQGPAPAGRVEPARGPSVIGPHIRVQGELLGDEDVLV
                     EGRVEGKVLLAREFRVAPGGVVVADVHAGTVVIAGQVVGDVSATERVEILPSGSLEGN
                     IRAPKVAVGDGARFKGSVDMSAGSPPGGDAPS"
     gene            2606..4132
                     /locus_tag="PLB01_07910"
     CDS             2606..4132
                     /locus_tag="PLB01_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQQ77266.1"
                     /translation="MAPSAPVHGNWIAGKHVPARSGRTFENRNPADVTDLVGRFADSD
                     ERDVKDAVDAAAEAYKSWRLVPGPQRGEILYRLGEILRDRKEQYSRDMTREMGKVLKE
                     ARGDVQEAIDMAFLMGGEGRRLHGQTTPSELPNKFAMCIRQPIGVCAFITPWNFPMAI
                     PAWKSTAALVCGNTVVVKPATDTPLSVVNFAKALEDAGLPPGVFNVVTGSGSKVGNPL
                     LKDPRVRVVSFTGSTSVGRAINEACAPSFKHVHLEMGGKNPIIVMDDANLELAVDGAI
                     WGAFGTSGQRCTASSRLLVHRKVVKKFTAMLAERAAALKVGNGLDEKNEMGPAINESQ
                     LDTVLDYISIGKDEGAKLVAGGKRLTGKGHDKGWFVAPTVFGDGDRKMRIAREEIFGA
                     VTVVVPISSLEEAIDISNESEYGLSSAIFTQDINKAFVAMRDLEAGIFYVNLPTIGAE
                     THLPFGGTKQTGNGHREAGLAALDVFSEWKSIYVDYSGTIQKAQIDVPAGEASAKKKR
                     "
     gene            complement(4174..5397)
                     /locus_tag="PLB01_07915"
     CDS             complement(4174..5397)
                     /locus_tag="PLB01_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQQ77267.1"
                     /translation="MSTTRTAAQVSARGIAMPASPIRKLMPLANAAKARGVKVLHLNI
                     GQPDLETPEPMRRRLAELKDRVYAYSPSNGTPEYLDFLKTYYGRLGIALEPSQILATT
                     GGSEALLFAFMACSDPGGEILTVEPLYTNYRSFAAMAGLTLSPIVTRGDDGFHLPGRA
                     EWEKALTPKTRMVLLCNPNNPTGTVYTKDEIRLVAEFCRDHGLFFVSDEVYREFLYDG
                     RVSESALSLAGFEHEVVVVDSLSKRFSACGIRLGALVTRNEELAAATNRMAQGRLSPP
                     GLAQFIAPAAAALGPDYYRGVVTEYQKRRDTLFAGLSGIPGVFLRKPEGAFYLVARLP
                     IKDSDDFAAWLLTDFQKDGKTVMVAPASGFYVTPGLGKDEVRIAYVLKEDDLKDAIGI
                     LAAALPAYRKARGLA"
     gene            5439..6665
                     /locus_tag="PLB01_07920"
     CDS             5439..6665
                     /locus_tag="PLB01_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQQ77268.1"
                     /translation="MTPALWILPFAMLLLAIAVFPLLLPERWAHLKFQALVAGVVSLP
                     VLLRFALHGPGGLAQIAHEYISFVVLLGSLYVVASGIVVDGDVPATPRTNTLLLLMGG
                     VLASLIGTTGASMLLIRLVLDVNVERKQVSHTVLFFILIVSNIGGSLTPIGDPPLFLG
                     YLAGVPFFWTLVLLKQWAVALGVLLAAYYLIDRRFHARETRAALLDDALTIRPIRISG
                     KWNFVLLLGVVLATVFLPSPWRETASVACAAASFPLTTKGLRAGTGFTWHPILEVAVL
                     FAGIFVTMEPALVILRERAPTLGLTEPWQFFWATGALSSWLDNAPTYAAFFALARGLG
                     RPEVAGVPANVLEAVSAGAVFFGAMTYIGNGPNFMVRSIAVSRGVPMPSFFGYAWRAA
                     AVLLPLFVLETVLFFR"
     gene            complement(6679..7674)
                     /locus_tag="PLB01_07925"
     CDS             complement(6679..7674)
                     /locus_tag="PLB01_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQQ77269.1"
                     /translation="MSDTPRPARAIRADRGDVGERLDHVLLRHLTDLPEASRTKIQEW
                     IEGGFVTLAGRIPKKTSEKVKKGDVIEVVLPPPPPLRPDILAEDIPLSILFEDSEILV
                     LNKPAGLVVHPAVGHRQGTLVNALLHRSKDWAGPADRPGLVHRLDKDTSGVLIIAKTE
                     RAMSVLGRAMKERFLEKEYLAVVYGKPPLTKGRIDLGILRDPSNRKRMAASKSEGRPS
                     STFYENLSESSGNKAGLALLKCRLVTGRTHQIRVHLKALNLPIVGDAVYGSPRWKGLK
                     DETLKEACRTFPRQALHAWRLGIRHPATGAAMTFTAPVPEDLGGLIAAAGLRPPA"
     gene            complement(7671..8453)
                     /gene="lgt"
                     /locus_tag="PLB01_07930"
     CDS             complement(7671..8453)
                     /gene="lgt"
                     /locus_tag="PLB01_07930"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQQ77270.1"
                     /translation="MHPELFRIGSFALPSYGFMMAVAFVSGLWLLRRRAPSFGIETET
                     ASDIGVWLLLSGLVGAKLLLVIVEWPTYVRSWSDFASLGRAGGVFYGGLIGAVLATAI
                     LLRRRKISFWTFADAAAPSVALGQAFGRVGCFLAGCCWGRECSLPWAVTFTSPVAHEN
                     VGVPLGIPLHPTQVYEILGTLLLCVLLVRFERRAFSGETFARYVFGYALLRGTIEFFR
                     GDPRGEVLGVMSTSQFIALCGVLAGLAIFALRRRVPPAPKPA"
     gene            complement(8459..8959)
                     /gene="lspA"
                     /locus_tag="PLB01_07935"
     CDS             complement(8459..8959)
                     /gene="lspA"
                     /locus_tag="PLB01_07935"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQQ77271.1"
                     /translation="MTRRLPYLALALGVVVLDQATKAVVVAKIPLYKTIPVIRGFFDL
                     THLQNTGAAFGFLAAAGSARPLLVTLLALAVFAGVLAWSLTTPPEHRLLQSALGLIMG
                     GAVGNLIDRVRFSAVTDFLRFYVDRWEWPSFNVADSAISVGVVLLAWDIWRRPEPEPA
                     RKAART"
     gene            complement(8969..11935)
                     /gene="ileS"
                     /locus_tag="PLB01_07940"
     CDS             complement(8969..11935)
                     /gene="ileS"
                     /locus_tag="PLB01_07940"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQQ77272.1"
                     /translation="MSRDWKSTLLLPKTDFPMRGDLPKREPPRLARWQEGDLYGRIRA
                     ARKGAPAFLLHDGPPYANGRIHMGTAMNKILKDLVVRSRTLDGFDAPYVPGWDCHGLP
                     IELKVDKELGPKKREMSDVAIRDACRASAEKWMDVQRTDFMRLGVLGAWTKPYRTMDF
                     SYQSEIARAFGRFVEKGLVSFGFKAVLWCVHDRTALAEAEIEYEDKTDWSIYVAMPLE
                     KNSTEGAWAGVAEVVQAPRLYAVIWTTTPWTLPANRAIALGAAVDYVFLRRESDPSAL
                     YLVADALVPQVTAALGWRDAHPLPHSKKNGAALAGAKLLYTRPFSNDPKDNFGFLLGD
                     HVSTTDGTGLVHTAPGHGRDDYEVVRRAGFKVDDPALCPVDEGGFYDVNAPEFLRGKR
                     VVIPKTPADDANTAVLEALKDGRAGAAVLLSQRSSPHSYPHCWRCKNPVVFRATHQWF
                     IDLGALRDRAMGEIRGRVEWIPSYSENRIGAMVDNRLEWTISRQRRWGSPITFLRCVD
                     CKQDGVVSHFPRVEGNVSEKEKREREDFFERVREAFREHGANAWYDDAFPPSYFLSKS
                     SSSSPSSSSSSASCSVCGGTNFEKLKDILDVWFDSGVSHAAVLRSGDYGLADPYTAQP
                     PVPVMYLEGHDQHRGWFQSSLLTSVALTGRAPYDAVLTHGFVVAGDGRKMSKSLGNTI
                     EPQDLLKTEGADVIRLWVASLDYTNDDPLSKEILQRTSEAYRKIRNTARFLISNLFDF
                     DPAKDAVPDARLEPLERWALDAAARFAAEARAAYARYEFHAVVRRLLDLVTTDLSAFW
                     CDVRKDAFYVLAPNDPVRRSAQTAAWKLVETIALALSPICPFTAEEIFESLPGNAKDP
                     SALFLKSWGDLKTSEISSNEREAWGKVLALRAEFLQSLEPLRRENKVGSASQAAAAVG
                     RSEEVDRALALLHLSEERLADVFGTPSVAREGDADSVLARAAEGTKCPRCWQVRKDVE
                     PGEDGICARCRAVVGG"
     gene            12015..12959
                     /locus_tag="PLB01_07945"
     CDS             12015..12959
                     /locus_tag="PLB01_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQQ77273.1"
                     /translation="MASLKTGVVGCGYLGRHHARILSTLEGSSPAGFVETDDAAAAAI
                     EAAHGAAGMVRRRSIRDLLDAGATAVVVSSPTVTHAAVAEELLLGGADVMVEKPITAT
                     LAEADRLVAIARETGRVLQVGHIERFNPAVAAVRPLVTDVKFIEAHRLGVFVPRALDV
                     DVVLDLLVHDLDIALDLVGRPVVEIRAVGVPILSNKIDIASVRLSFEGGCVANLTASR
                     VSQEKTRKFRFFQPDWYFSIDTQNRDVTAFRLDRMTGPPRIVPWPVAVEPQDALTAED
                     AAFLKACRERSEPEVTGEAGRAALKLALDILEEMGRNR"
     gene            12962..13882
                     /gene="mtnP"
                     /locus_tag="PLB01_07950"
     CDS             12962..13882
                     /gene="mtnP"
                     /locus_tag="PLB01_07950"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HQQ77274.1"
                     /translation="MKRSKSTKTKKKISSSGRGKKTPRIGLIGGSGLYALPGLKITDE
                     VELTTPFGEPSDSYFLGTLSSVPVTFLARHGRHHHLLPTEVNYRANVWGLKSLGCSWV
                     ISASACGSMKEAYKPTDIVLPDQFIDLTKRRTSTFFGEGCVGHVSFSHPVSTTLLDAL
                     EAAGKAEKAVVHRGGTYVCMEGPQFSTKAESLLYRSWGVDVIGMTNATEAKLCREAGI
                     AYASACLVTDYDCWHEEEGPVSVEAVLAVLKANAATANRVLANAVVRLDPARPSDCAD
                     ALAFAILTHPTRVPVRTRSRLALFTQKGRP"
     gene            13879..14883
                     /locus_tag="PLB01_07955"
     CDS             13879..14883
                     /locus_tag="PLB01_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQQ77275.1"
                     /translation="MTPARIVVTGSVAFDYLMTFPGKFLDVVVPDRMSRLSVSFLVDS
                     MRRVRGGVAPNIAYGLALFGLKPVIMATAGVDALDYARWLAEEGVDTSELHISDDLFT
                     ASFFVSTDEVQNQIATFYAGAMTRAVDLSFRAFGPGTVSLAVIAPNAPEAMMKYAAEC
                     RTLRIPFVFDPSQQVARLSGGEILAGLDGAAVLVANEYEFGVIEKKTGLSEAEVLKQV
                     PVLIVTRGNEGSTIAIRGDGTEEAATHVIPAAKLRTTAVDPTGVGDAFRAGLLAARQR
                     GLPWEVAGRVGSVAAVYALETIGAQPKRYGMGDFRDRYRENFGADGVHATVDRLLAGA
                     "
BASE COUNT         2331 a         5043 c         5112 g         2453 t
ORIGIN      
        1 tcggcgcgac ggccggccag ggcgtcgtct ggtacacggg ccagttctac gcgctgttct
       61 tcctcacgat cacgctcaag ttcgactggg cgacggcgta cttgctcatc gcgctctcgc
      121 tcgtgatcgg aacgccgttc ttcatcgtgt tcggcgcgct ctcggacaag atcggccgcc
      181 tcaagatcat cctcaccggc tgcgcgatcg ccgccatcac gtacatcccg ctcttcaagg
      241 ccctcacgca ctacgggaac ccggccctcg agaagtacca gaactcgacg ccgatctcga
      301 tcgcggggac gaactgccag atgcacatct tcgtgacgcc gttcacgaag ttctcggcgt
      361 gcgacaaggc ccaggacttc atgtcgaaga acggcctttc gttcgcgacg cttccgcccc
      421 aggccgacga cgtcgtcatc aaggtcggca gcatcgagat caagggctac gacgaggcga
      481 agctcaagga ggccttcaag cagaccggct atccgaaccc ggcgttcaag aaggacgaga
      541 tgaacttcgg gatggtgctc ctcatcctcg tcatcatggt cctctacgtc acgatggtgt
      601 acgggccgat cgcggcgttc ctcgtcgagc tgttccccgc gaacatccgg tacacctcga
      661 tgtcgctgcc gtaccacatc ggcaacgggt ggttcggcgg catgctcccg ctgatcgcga
      721 ccgcgtgggt cgcggcctcg ggcaacatct accagggcct ctggtacccg atcatcgtgt
      781 ccgtgatcac ggtggtcatc ggcggcctgt tcctgcgcga gacgaaggac gtcaaaatcc
      841 acgaagaagt ctgaccgaag gatttcttcg aagatttcat gggccgggcg gtgcttgccg
      901 cccggcttct tttttcttcg aagggagcga ttccgcggag tctgccggta gacttgccgc
      961 tccatgccgc gtcgcgagcc gatcgcccct ctcgccttcc tccagcagtt catgcggacc
     1021 gaggccgcgt ccggagtcct ccttctcgcg gccgccgtgg ccgcgatcgt tttcgcgaat
     1081 tcgccgctcc agtcggtcta cgcccacgcg acgcaccttc ctctccggtt cggcgacggt
     1141 ccctggcgcg tcgacacgac gctcctgcac gcggtcaacg acgggctgat ggccgtcttc
     1201 ttcctcgtcg tggggctgga gatcaagcgc gagttcctga cgggggagct ctccgggaag
     1261 ggcgctgcga tcctccccgt ggcggcggca tgcgggggag ccgtgatccc cgcgtcgatc
     1321 tacgcggcgt tcaacgccgg gacgcccgcg gcgcaaggct ggggcatccc gatggcgacg
     1381 gacatcgcgt tcgcggtcgg cgttctcgcg ctgctcggcc ggcacgtggc gcccgttttc
     1441 aaggtgtttc tcaccgccgt ggcggtcgtc gacgacctga tcgcggtgct cgtgatcgcg
     1501 ttcttctaca cgtccggcct cgatctccgc gcgctggccg tcgtcgccgg cgcgtgcgtg
     1561 ctcctttacg ccctgaaccg ctccggcgtc caggccctcg ctccgtatct cctcgtcggg
     1621 ctcgtcctct gggccgcgat ggcgaaatcg ggcgtccacg cgacgatcgc gggcgtcctg
     1681 ctcggcatga gcattcccgc ggcgggcgcc gaaacgtcgc cgctccgccg gctcgagcac
     1741 gcgttgcacc cctgggtcgc ctgggggatc atgccggtct tcgccctcgc gaacgcgggc
     1801 gtgaccgtgc ctgccgggaa gctgggcgcg atgctgacgt ctccaatcgc gctcggcatc
     1861 gtcctcgggc tcttcgtggg caagcaggtc gggatcttcg ggacgacgtt ccttctcgcc
     1921 cgtctcgggg tcggagcgat tccgctcgac gcacgggcgc gccggtcgct ctggggggtc
     1981 gcgctcctcg gcgggatcgg gttcacgatg tcgctcttcg tgagcggact cgccttcacg
     2041 gacgaggcga tggcggacgt ctccaaggcg gcgatcctcg ccggctcgct gatatcgggt
     2101 ctcgccggtg cagccgttct cgcgagtgtt ccggagccgg tggcgggaga cgcggacgcc
     2161 ggttagactc ctgcgtcgag gatggccatc ttcgggaaaa gctcgcaagg cccggcaccc
     2221 gcgggccgcg tcgagccggc acgcgggccg tccgtcatcg ggccccacat tcgcgtacag
     2281 ggcgagcttc tcggagacga ggacgtcctg gtggaagggc gcgtggaggg gaaggtcctc
     2341 ctcgcgcgcg agttccgtgt cgccccgggc ggcgtcgtcg tcgcggacgt gcacgccggc
     2401 acggtcgtga tcgcgggcca ggtcgtcggc gacgtgagcg cgaccgagcg cgtcgagatc
     2461 ctgccctccg gctccctgga aggaaacatc cgcgccccca aggtcgccgt cggcgacggg
     2521 gcgcgattca aggggagcgt cgacatgagc gccgggtccc cccccggcgg cgacgccccc
     2581 agctgatcga aaggaagctc cgcacatggc accgtccgca cccgttcacg gcaactggat
     2641 cgccggcaag cacgttcccg cccgctccgg ccgcacgttc gagaaccgca accccgccga
     2701 cgtgaccgac ctcgtcggcc gcttcgcgga ctccgacgag cgcgacgtca aggacgccgt
     2761 cgacgcggcg gcggaggctt acaagagctg gcgcctcgtt ccgggcccgc agcgcggcga
     2821 gattctctac cggctcggcg agatcctccg cgaccgcaag gaacagtatt cgcgcgacat
     2881 gacgcgcgag atgggcaagg tcctcaagga agcccgcgga gacgtgcagg aggccatcga
     2941 catggccttc ctcatgggcg gcgagggccg ccgcctgcac ggacagacga cgccgtcgga
     3001 gctcccgaac aagttcgcca tgtgcatccg gcagccgatc ggcgtctgcg cgttcatcac
     3061 gccctggaac ttcccgatgg cgatcccggc gtggaagtcc acggcggccc tcgtctgcgg
     3121 gaacacggtg gtcgtgaagc ccgccacgga cacgccgctc tccgtcgtga acttcgcgaa
     3181 ggcgctcgag gacgcgggcc ttcctccggg cgtcttcaac gtcgtgacgg gatcgggctc
     3241 gaaggtcggg aacccgctcc tcaaggaccc gcgcgtgcgg gtcgtctcct tcacgggctc
     3301 gacgtccgtc ggccgcgcga tcaacgaggc ctgcgcaccg tccttcaagc acgtccacct
     3361 cgagatgggc ggcaagaacc cgatcatcgt gatggacgac gcgaacctcg agctcgccgt
     3421 cgacggcgcg atctggggcg cgttcgggac gagcggacag cgctgcacgg cctcctcgcg
     3481 cctcctcgtc caccgcaagg tcgtcaagaa gttcacggcg atgctcgccg agcgcgccgc
     3541 cgcgctgaag gtcggcaacg gtctcgacga gaagaacgag atgggcccgg ccatcaacga
     3601 gagccagctc gacacggtcc tcgactacat ctcgatcggc aaggacgagg gagcgaagct
     3661 cgtcgccggc ggcaagcgcc tcacgggcaa gggccacgac aagggctggt tcgtcgcgcc
     3721 cacggtgttc ggagacgggg accggaagat gcggatcgcc cgcgaggaga tcttcggggc
     3781 cgtcacggtc gtcgtgccga tctcgtcgct cgaggaggcg atcgacatct ccaacgagtc
     3841 ggagtacggc ctctcgtcgg cgatcttcac gcaggacatc aacaaggcct tcgtcgcgat
     3901 gcgcgatctc gaggccggca tcttctacgt gaacctcccg acgatcggcg cggagacgca
     3961 cctcccgttc ggcggcacga agcagaccgg caacggacac cgcgaggcgg ggctcgcggc
     4021 gctggacgtc ttcagcgagt ggaagtcgat ctacgtcgac tactccggga cgatccagaa
     4081 ggcgcagatc gacgtcccgg cgggcgaggc gtccgcgaag aagaagagat gagagaagga
     4141 tttcctagaa ggtgaagagg gaagaggcct cgctcaggcg aggcctcttg cttttcgata
     4201 ggccggcagc gcagcggcga ggattccgat cgcatccttc aggtcgtctt ccttgaggac
     4261 gtaggcgatc cggacctcgt ccttgccgag ccccggcgtc acgtagaagc cgctcgcggg
     4321 cgcgaccatg acggtctttc cgtccttctg gaagtccgtg aggagccagg ccgcaaagtc
     4381 gtcactgtcc ttgatcggaa ggcgcgcgac gaggtagaag gcgccctcgg gcttcctcag
     4441 gaagacgccg ggaatgcccg agaggcccgc gaagagcgtg tcccgccgct tctggtactc
     4501 cgtcacgacg ccgcggtagt agtccgggcc cagcgcggcg gccgccggcg cgatgaactg
     4561 cgcgaggccg ggcggggaga gccgcccctg cgccatgcgg ttcgtggctg cggccagttc
     4621 ctcgttacgg gtgacgagcg cgccgaggcg gatgccgcag gcggagaacc gcttcgacag
     4681 cgagtcgacg accacgacct cgtgctcgaa gccggcgagg gagagcgcgc tctccgacac
     4741 gcggccgtcg tagaggaact cgcggtaaac ctcgtccgag acgaagaaga gtccgtggtc
     4801 gcggcagaac tccgcgacga ggcggatctc gtccttcgtg tagacggtcc cggtcgggtt
     4861 gttcgggttg cagaggagga ccatccgcgt cttcggcgtg agggccttct cccactccgc
     4921 gcggccgggc aggtggaagc cgtcgtcgcc gcgcgtgacg atgggactga gcgtgaggcc
     4981 cgccatcgcc gcgaacgagc ggtagttcgt gtagagcggc tcgacggtga ggatctctcc
     5041 gccggggtcc gagcaggcca tgaacgcgaa gagcagcgcc tcgctgccgc ccgtcgtcgc
     5101 gaggatctgg gacggttcga gagcgatgcc gagccgcccg tagtacgtct tcaggaagtc
     5161 caggtactcg ggcgtgccgt tcgaaggcga gtacgcgtag accctgtcct tcagctcggc
     5221 gaggcggcga cgcatcggct ccggcgtctc gaggtcgggc tggccgatgt tcaggtgcag
     5281 gactttcacg ccgcgcgcct tcgccgcgtt cgcgagcggc atcagtttgc ggatggggga
     5341 ggcgggcatt gcgatgccgc gcgcggaaac ctgggcggcg gtccgggtgg tgctcatggc
     5401 ccgtcagact agcaaatgcg cgaaggtaga ctccgcccat gacgccggcg ctctggatcc
     5461 tcccattcgc gatgctgctc ctcgcgatcg cggtgtttcc gctcctcctt ccggagcgat
     5521 gggctcacct gaaattccag gcgctcgtgg caggtgtcgt gagcctcccc gttctcctgc
     5581 gattcgctct ccacggcccg ggaggcctcg cccagatcgc tcacgagtac atctcgttcg
     5641 tcgtcctcct gggctccctc tacgtcgtgg cgtccggaat cgtcgtcgac ggcgacgttc
     5701 cggccacgcc gcgaacgaac accctgctgc ttctcatggg aggcgtcctt gcgtccctca
     5761 tcgggacgac gggcgcctcg atgctcctga tccgtctggt cctcgacgtc aacgtcgagc
     5821 ggaagcaggt ctcgcacacg gtcctcttct tcatcctcat cgtctcgaac atcggcggct
     5881 cgctgacgcc gatcggggat ccgccgctct tcctcggata cctcgcgggc gttcccttct
     5941 tctggacgct cgtcctcctg aagcagtggg cggtggctct gggcgtcctg ctcgccgcct
     6001 actacctgat cgaccggcgc ttccacgcgc gcgagacgcg ggcggccctt ctcgacgacg
     6061 cgctcacgat ccgaccgatc cggatctcgg ggaagtggaa cttcgtcctt cttctgggcg
     6121 tggttctcgc gacggtcttc ctgccgtctc cgtggcgcga gaccgcctcc gtcgcgtgcg
     6181 ccgcagcgag cttccccctc acgacgaagg gcctccgggc gggaaccggg tttacgtggc
     6241 atcccattct cgaggtcgcg gtgctcttcg ccggcatctt cgtcacgatg gagccggcgc
     6301 tcgtcatcct ccgggagcgc gccccgacgc tcgggctgac cgagccatgg cagttcttct
     6361 gggccaccgg cgctctctcg tcctggctcg acaacgcccc gacgtacgcc gcgttcttcg
     6421 cgctcgcccg cgggctgggc cggcccgagg tggccggtgt gccggccaac gtcctcgaag
     6481 cggtctcggc gggagccgtc ttcttcggcg cgatgacgta catcgggaac gggccgaact
     6541 tcatggtgcg gtccatcgcg gtctcgcgcg gagtcccgat gccgtcgttc ttcggctatg
     6601 cgtggcgcgc cgcggccgtt ctcctgccgc ttttcgtgct cgagaccgtg ctgttcttcc
     6661 gctgagcggg cgccggcgtc aggccggcgg acggagaccg gcggccgcga tcagcccgcc
     6721 gaggtcctcc ggcacgggcg ccgtgaacgt catcgccgca ccggtggccg ggtgccggat
     6781 tccgaggcgc cacgcgtgaa gcgcctgccg ggggaaagtc ctgcaggcct ctttgagcgt
     6841 ctcgtccttc aaacccttcc agcgcggcga gccgtagacg gcatcgccca cgatcggcag
     6901 gttcagcgct ttgaggtgca cgcggatctg gtgcgtccgg cccgtgacga ggcggcattt
     6961 caagagggcg agaccggcct tgtttccaga ggattccgag agattctcat agaaggtgga
     7021 agaggggcgc ccctcgcttt tcgacgcggc cattctcttt ctattcgaag gatcgcgcag
     7081 gatgccgagg tcgattcgcc ccttcgtgag cggcggcttt ccgtaaacca cggccagata
     7141 ttccttttct aagaatcttt ctttcatcgc ccgccccagg accgacatcg cacgctctgt
     7201 cttcgcaatg atcagcaccc ccgacgtgtc cttgtcgagc cggtggacga ggccggggcg
     7261 gtccgcgggg cccgcccagt ccttcgaacg gtgcagcagc gcattcacga gcgtcccctg
     7321 gcggtggccg acggccggat gcacgacgag gccggcgggc ttgttcagga cgaggatctc
     7381 ggagtcttcg aagaggatcg agagcgggat gtcttcggcg aggatgtcgg gccggagcgg
     7441 ggggggtgga gggaggacga cttcaatgac gtcacccttc ttcaccttct cggacgtctt
     7501 cttggggatc ctcccggcga gcgtgacgaa cccgccctcg atccactcct ggatcttcgt
     7561 gcgcgacgct tccggcaggt ccgtcaggtg gcgcaggagg acgtggtcga ggcgctcgcc
     7621 gacgtcgccg cggtcggcgc ggatggcccg ggccggccga ggcgtgtcgc tcacgccggc
     7681 ttcggggcgg gcgggacccg gcgccgcagc gcgaagatcg cgagccccgc gaggacgccg
     7741 cagagcgcga tgaactgcga ggtcgacatc acgccgagga cctcgccccg cgggtcgccg
     7801 cggaagaact cgatcgtccc gcgcaggagc gcgtacccga agacgtaccg ggcgaacgtc
     7861 tctccggaga acgcccgccg ctcgaagcgg acgaggagca cgcaaaggag gagcgtgccg
     7921 aggatctcgt agacctgcgt cgggtgaagg gggattccga gcgggacgcc gacgttctcg
     7981 tgggcgacgg ggctcgtgaa cgtgaccgcc cacggcaggg agcattcccg gccccagcag
     8041 cagccggcga ggaagcagcc gacgcggccg aaggcctgcc cgagggcgac ggacggggcc
     8101 gcggcgtccg cgaacgtcca gaacgagatc ttccggcgcc tgagcaggat cgcggtcgcg
     8161 agaactgcgc cgatgaggcc gccgtagaag acgcctccgg cgcgcccaag agaggcgaag
     8221 tcactccacg accggacgta ggtcggccac tcgacgatca cgaggagaag cttcgcgccg
     8281 acgagacccg acaggagcaa ccagacgccg atgtccgacg ccgtctcggt ctcgattccg
     8341 aacgacgggg cccggcggcg gaggagccag agcccggaga cgaaggccac ggccatcatg
     8401 aatccgtagc tcgggagcgc gaacgagccg atgcggaaga gctcgggatg catgaaggtc
     8461 aggtcctcgc ggccttgcgc gccggctcgg gctcggggcg gcgccagatg tcccacgcca
     8521 gcaggacgac acccacggaa atggcactgt cggcgacgtt gaaggacggc cattcccagc
     8581 ggtcgacgta gaagcgcagg aagtcggtga cggccgagaa gcggacgcgg tcgatgaggt
     8641 ttccgacggc cccgcccatg atcagcccga gcgcggactg gagaaggcgg tgctccggcg
     8701 gcgtcgtcag cgaccaggcg agaacgcctg caaagaccgc gagggcgagc agcgtgacga
     8761 gaagcgggcg ggccgagccc gccgcggcga ggaagccgaa ggctgcgccc gtgttctgga
     8821 ggtgggtgag gtcgaagaaa ccccggatca cggggatcgt cttgtagagc gggatcttcg
     8881 cgacgacgac cgccttcgtg gcctgatcga ggacgacgac gcccagcgcg agggcgaggt
     8941 aggggaggcg ccgcgtcacc gggggattct aaccgccgac gaccgcgcgg cagcgtgcgc
     9001 agatcccgtc ttcacccggc tcgacgtcct tccggacctg ccagcagcgc gggcacttgg
     9061 tgccttcggc ggcgcgcgcg agcacggagt cggcgtctcc ctccctcgcg accgatggcg
     9121 tcccgaacac gtcagccagt ctttcttctg aaaggtgaag aagggcgagc gcgcggtcga
     9181 cctcctcgct gcgcccgacg gcggcggcgg cctgggaggc ggagccgacc ttgttttcgc
     9241 gcctcagggg ctcgagggat tgcaggaact ctgcccgaag cgcaaggacc tttccccacg
     9301 cctctctttc attcgaagaa atctccgaag tcttcaggtc cccccagctc ttcaggaaca
     9361 gcgcggacgg gtccttcgcg tttccgggca gcgactcgaa gatctcctcg gccgtgaacg
     9421 gacagatggg ggagagagcg agcgcgatcg tctccacgag cttccaggcg gccgtctgcg
     9481 cggagcggcg cacggggtcg ttcggcgcga ggacgtagaa ggcgtccttg cgcacgtcgc
     9541 accagaacgc cgagaggtcg gtcgtgacga ggtcgaggag gcggcggacc acggcatgga
     9601 actcgtaccg ggcgtatgcc gcgcgggcct cggccgcgaa gcgggccgca gcgtccaggg
     9661 cccaccgctc gagcggctcg agccgggcgt ccgggactgc gtccttcgcg gggtcgaagt
     9721 cgaagagatt cgaaatgagg aaccgcgccg tattccggat cttgcgatac gcctcggacg
     9781 tccgctggag gatctccttc gagagcgggt cgtcgttcgt gtagtcgagc gacgcgaccc
     9841 agaggcggat cacgtcggca ccttcggtct tgagaagatc ctggggctcg atcgtgttcc
     9901 cgagcgactt cgacatcttc cggccgtcgc ctgcgacgac gaacccgtgg gtgagcacgg
     9961 cgtcgtacgg cgcgcggccc gtcagcgcga ccgacgtgag aagcgaggac tggaaccagc
    10021 cgcggtgctg gtcgtgaccc tcgaggtaca tcacgggcac cggcggctgc gccgtgtacg
    10081 ggtccgcgag gccgtagtcg cccgaccgga gcacggccgc gtgcgagacg cccgagtcga
    10141 accagacgtc gagaatgtcc ttcagcttct cgaagttcgt cccgccgcag acggaacagg
    10201 aggccgaaga ggaagacgaa gagggagacg aagaggaaga tttgctaaga aagtaagacg
    10261 gggggaacgc gtcgtcgtac caggcgttcg ctccgtgctc gcggaacgcc tctcttaccc
    10321 tttcgaagaa atcttctctt tctctctttt ccttttcgct gacgttccct tcgacccgcg
    10381 gaaagtggga gacgacgccg tcttgcttgc agtccacgca gcgcaggaac gtgatcggcg
    10441 agccccagcg ccgctggcgg gagatcgtcc actcgaggcg gttgtcgacc atcgcgccga
    10501 tgcggttctc gctgtaggag gggatccact ccacgcggcc gcggatctcg cccatcgcgc
    10561 ggtcgcgcag cgccccgagg tcgatgaacc actggtgcgt cgcccggaag acgaccgggt
    10621 tcttgcagcg ccagcagtgg gggtaggaat gcggcgaaga tctctgcgaa aggaggacgg
    10681 cggcgccggc gcgcccgtcc ttgagggcct cgaggaccgc ggtgttcgcg tcgtcggcgg
    10741 gggtcttcgg gatgacgacg cgctttcccc tgaggaattc cggggcgttg acgtcgtaga
    10801 agccgccctc gtccacgggg cagagcgccg ggtcgtccac cttgaagccg gcccgccgca
    10861 cgacctcgta gtcgtcgcgg ccgtggccgg gcgccgtgtg gacgaggccg gtgccgtccg
    10921 tcgtcgagac gtggtcgccg aggaggaatc cgaagttgtc cttcggatcg ttcgagaaag
    10981 gcctcgtgta gaggagcttc gccccggcga gcgcggcgcc gtttttcttc gagtggggaa
    11041 gagggtgggc gtcgcgccag ccgagcgcgg ccgtcacttg cgggaccagc gcgtcggcga
    11101 cgagatagag cgccgaggga tcggattctc ttctgaggaa gacatagtcg acggcggccc
    11161 caagggcgat cgcacggttc gccggcagcg tccacggcgt cgtcgtccaa atcaccgcat
    11221 acagacgcgg cgcctgcacg acctctgcga caccggccca cgccccttct gtggaattct
    11281 tctccagcgg catcgcgacg tagatcgacc agtccgtctt gtcctcgtac tcgatctccg
    11341 cttccgcgag agccgtccgg tcgtggacgc accagagcac ggccttgaag ccgaagctca
    11401 cgaggccctt ttcgacgaac cgcccgaacg cccgggcgat ctcggactgg taggagaagt
    11461 ccatcgtccg gtagggcttc gtccacgcgc cgagcacgcc gaggcgcatg aagtcggtcc
    11521 gttggacgtc catccacttc tcggcggagg cgcggcacgc gtcgcggatc gcgacgtcgg
    11581 acatctcccg cttcttcggg ccgagttcct tgtccacctt gagctcgatc gggagcccgt
    11641 gacagtccca gcccggcacg tagggggcgt cgaatccgtc gagcgtccgg gaccggacga
    11701 cgagatcctt gaggatcttg ttcatcgcgg tgcccatgtg aatgcgcccg ttggcgtacg
    11761 gcgggccgtc gtggagcagg aaggcgggcg cgcccttccg cgcggcgcgg atgcggccgt
    11821 agaggtcgcc ctcctgccag cgggcaaggc gcgggggctc gcgcttcggg agatcccctc
    11881 gcatggggaa gtccgtcttc gggagcagga gggtggattt ccagtcgcgg ctcattcggg
    11941 ccgccgaggt tacatgattc agatggatca ggacgcgttc cggagccctg ttcgtccctt
    12001 acactccgcc gcgcatggcg tcgctcaaga ccggtgtcgt cggctgcggc tacctgggcc
    12061 gccaccacgc ccgcatcctg tccacgctcg agggttcgag cccggccggc ttcgtcgaga
    12121 cggacgacgc cgcggcggcg gcgatcgaag ccgctcacgg ggccgcgggg atggtccgcc
    12181 ggcggtcgat ccgggatctt ctcgacgcgg gggccacggc cgtcgtcgtc tcttctccca
    12241 ccgtcacgca tgccgccgtc gccgaggagc tcctcctcgg tggagcggac gtgatggtcg
    12301 agaagccgat cacggcgacg ctcgccgagg cggaccggct cgtcgcgatc gcacgcgaga
    12361 cgggccgcgt cctgcaggtc gggcacatcg agcgcttcaa tcctgccgtc gcggccgtcc
    12421 gcccgctcgt cacggacgtg aagttcatcg aggcgcaccg cctcggggtc ttcgtcccgc
    12481 gtgccctcga cgtggacgtc gtcctcgacc tcctcgttca cgacctcgac atcgcgctgg
    12541 acctcgtggg ccggccggtc gtcgagatcc gcgccgtcgg cgtcccgatc ctctcgaaca
    12601 agatcgacat cgcctccgtg cggctgtcgt tcgagggcgg gtgcgtcgcg aacctgacgg
    12661 cgtcgcgcgt ctcgcaggag aagacgcgga agttccggtt cttccagccg gactggtact
    12721 tctcgatcga cacccagaac cgcgacgtga cggccttccg cctggaccgg atgacgggac
    12781 cgccgcgcat cgtgccctgg cccgtcgccg tcgaacccca ggacgccctg acggccgagg
    12841 acgcggcgtt cctgaaggcc tgccgcgagc gcagcgagcc ggaagtcacg ggcgaagccg
    12901 gccgcgcggc gctgaagctc gcgctcgaca tcctcgaaga gatgggccgg aaccggtgac
    12961 gatgaagcgt tcgaagagca cgaagacgaa gaagaagatt tcttcgagtg gaagagggaa
    13021 gaagacgccg cgcatcggcc tcatcggcgg ctccggcctg tacgcgcttc ccgggttgaa
    13081 gatcaccgac gaagtcgagc tgacgactcc cttcggagag ccctcggact cctactttct
    13141 cggaactctt tcttctgtcc ccgtgacttt tctggcccgc cacggacgtc accaccatct
    13201 cctgcccacg gaggtcaact accgtgccaa cgtctggggg ctgaaatccc tcggctgctc
    13261 ctgggtcatc tccgcgtccg cgtgcggctc gatgaaggaa gcgtacaagc cgaccgacat
    13321 cgtcctgccg gatcagttca tcgacctcac gaagcgtcgc acctcgacgt tcttcggcga
    13381 gggctgcgtc ggccacgtgt cgttctcgca ccccgtctcg acgacgcttc tcgacgccct
    13441 cgaggcggcg gggaaggccg agaaggccgt cgtccaccgg ggcgggacgt acgtctgcat
    13501 ggaagggccg cagttctcga cgaaagccga gtcgcttctc taccggtcct ggggagtgga
    13561 cgtcatcggc atgacgaacg ccacggaagc aaagctctgc cgtgaagcgg ggatcgcgta
    13621 cgcctcggcc tgcctcgtga cggactacga ctgctggcac gaggaggaag ggccggtcag
    13681 cgtcgaggcc gtcctcgccg ttctgaaggc gaacgcggcg accgcaaacc gcgtcctcgc
    13741 gaacgccgtg gtccggctgg acccggcgcg gccgagcgac tgcgccgacg ccctcgcctt
    13801 cgcaatcctc acgcacccga cgcgcgttcc cgtccgcacc cggagtcggc tcgcgctctt
    13861 cacgcagaaa ggacgtccat gacgcccgcc cgcatcgtcg tcaccggatc ggtggccttc
    13921 gactacctca tgacgttccc ggggaagttc ctcgacgtcg tcgtcccgga ccggatgagc
    13981 cgtctctccg tctcgttcct cgtcgactcg atgcgccgcg tccgcggcgg cgtcgccccg
    14041 aacatcgcgt acggcctcgc gctcttcggc ctcaagcccg tgatcatggc gacggcgggg
    14101 gtcgacgcgc tcgactacgc gcggtggctc gcggaagagg gcgtcgacac gtccgagctc
    14161 cacatcagcg acgacctctt cacggcgtcg ttcttcgtct cgaccgacga ggtccagaac
    14221 cagatcgcga cgttctacgc gggcgcgatg acccgtgcgg tcgacctctc gtttcgcgcc
    14281 ttcgggccgg ggaccgtctc gctcgcggtg atcgcgccga acgcgcccga agcgatgatg
    14341 aagtacgccg cggagtgccg gacgctccgc atcccgttcg tgttcgaccc gagccagcag
    14401 gtcgcgcgcc tcagcggcgg cgagatcctc gccggcctcg acggcgccgc cgtcctcgtc
    14461 gcgaacgagt acgaattcgg cgtcatcgag aagaagacgg gcctgtccga agccgaggtc
    14521 ctgaagcagg tcccggtcct catcgtcacg cggggaaacg agggctcgac gatcgcgatc
    14581 cgcggcgacg ggacggagga ggccgcgacc cacgtcatcc ccgcggcgaa gctgcgcacc
    14641 accgccgtgg acccgacggg cgtcggggac gcgttccggg ccgggctcct cgccgcccgc
    14701 cagcgtgggc tgccctggga ggtcgccggc agggtcggca gcgtcgcggc cgtgtacgcc
    14761 ctcgagacga tcggcgccca gccgaaacgc tacggcatgg gcgatttccg cgaccgctat
    14821 cgcgagaact tcggggccga cggcgttcac gcgaccgtgg accgcctgct cgcaggagcc
    14881 tgaactgtca acgctcctta gaaatgtccc ctgaaatagc tcgttagaaa tgtcccctc
//
LOCUS       DAOMFY010000073        14927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1236_length_14927_cov_7.778039, whole genome shotgun sequence.
ACCESSION   DAOMFY010000073 DAOMFY010000000
VERSION     DAOMFY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_14927_cov_7.778039"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1434
                     /locus_tag="PLB01_07960"
     CDS             124..1434
                     /locus_tag="PLB01_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ77276.1"
                     /translation="MDVTSFFQTPEKADAGLSPLARGVVGSEILRIAGEIRALQATGR
                     PVCNLTIGDFDSRQFPIPDGLLAATKDALETGQTNYPPAEGILPLREEVVKLYARDLG
                     LSYPVESVVIAAGARPLLYGTYRTLLEPGDGAIYPVPSWNNNHYAYLSGARGIPIRVE
                     AKSNFFPTPDQVRPHVPGARLLILNSPLNPTGTAISKQALTDLTEMVVEENRRRDSEG
                     RRRPLYLVYDQVYWTLVFGKTEHHTPVGLVPESAPYVIFLDAISKAFCATGMRVGWGV
                     MPPPIRRRMSDILGHVGAWAPKAEQVGVAKFLAQPEAVRAYRDGVRKKLAERLEALYD
                     GVMSMKKEGFPVDAIEPQGAIYLSVRFDLFGRTVRGVPVKTNDDVRRLLLEEAGLGVV
                     PFQAFDFEGDTGWFRLSVGAVSMDEIAAAFPRLRAMLAEAPAAV"
     gene            1470..2126
                     /locus_tag="PLB01_07965"
     CDS             1470..2126
                     /locus_tag="PLB01_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ77277.1"
                     /translation="MEQDKKSERSRKAILEAALDLFSSQGYRGTSIRDIAERAGVSTG
                     NVYHHFADKETIFQTVLGQYWKAIESPDFPFNKALASGTFPDNLEELAAAAKESVRVW
                     RRHVALIYVDVVELSGNHIRKFYSEMAVRFERFLVSHGDSVSGEIRPGVPVPTAVMLA
                     SRVFLQYYAVEILFGVPVQFGKDSDLVIREIAEILRHGVLKEERLAASAPVPLSRARR
                     "
     gene            complement(2123..2989)
                     /locus_tag="PLB01_07970"
     CDS             complement(2123..2989)
                     /locus_tag="PLB01_07970"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ77278.1"
                     /translation="MSAAPPPAPLREGRLVHGGHGYHWELYGAGGRETVCLFNGLAMS
                     TKSWLSFLPELTGERDVLLYDYLGQGASDDAELPGAPISSFGDALAAILDAEGISRIH
                     AIGVSYGGFVAAEFARRHPARAASLTLSGILLTQETLFAMYQDVSLRFYAGGPPLFDL
                     YTRYLYEKIFAEAFVARAGPKLESMRQGFHDRYAGRIDALVRLTKAQDPFFAALGENA
                     ESYAAIPCRVLVLAGAEDRAIPPPQQRRIPTVIPHAVYEEIPGAGHVVYLEKPEVFWP
                     RVRRFLTGTEAV"
     gene            complement(2986..3804)
                     /locus_tag="PLB01_07975"
     CDS             complement(2986..3804)
                     /locus_tag="PLB01_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ77279.1"
                     /translation="MSQPLLTHRVDGEGPPLLLLNGGMMSLSAWEPIARRFAERHRVI
                     RCDFRGQLLSPGPPRASMEEHADDVAALLESLSVGPVDVVCASYGAYAGLLLAARRPE
                     RVRSVVAATVTDVAREGDAALGEAGARLAAAVRAAARGGDRTEAYDAIVELAYTPEWR
                     VAHADELAARRGQVGLLPETWFTGLEGLLAALDKVDLRPALGRISCPVLVVAAERDAA
                     MPRERTKAVADGIPVAHFVVVPGAGHALVVEREDEFVLLVERFLARVHRSGGAA"
     gene            complement(3807..5438)
                     /locus_tag="PLB01_07980"
     CDS             complement(3807..5438)
                     /locus_tag="PLB01_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQQ77280.1"
                     /translation="MISGDVLGERARLSPEKTALVEVATGRRFTYAELDARARGAART
                     LTEGLGLKKGDRMALLAGNCVEFLDVFFACGKNGVVLVPLNTRQTAGELIGVLEDAEP
                     KALIYGAAFEETVEDLLRKVRGVTPVLLDAPAQRGARAAGAPNARTTFKEIFSSPPAA
                     APAAAAEVPSPGPEDLYCLLYTSGTTGKPKGVMVPHRMVAWNAVNTVVSWQLREDDVS
                     PIYTPLYHAGGLGAFLTPVFAIGGTIVLHAGFDAAEVLATIGKERCTVVLGVPTIFRM
                     LQEHPSWASADFSSVRYFISGGAPLPLDIIETWHRRGANFKQGYGLTEVGVNCFAMSV
                     EESVRKAGSIGRPMMFTEAKLTGEDGREVPVGEVGELLLRGPHVCRGYFRNPEATAAS
                     LDAEGWFHTGDLAKKDEDGFFTIAGRKKDMLISGGVNVYPAEIEGVLFQHPRVKDVAV
                     VGVPDEKWGEVGVAFVVRREEGREEKGEEKISLKVIGEMAELAAFVEARLAKYKVPRE
                     FIFVASLPRTPYGKVVKGDLRSLYLSKKSSTNEAR"
     gene            complement(5435..6406)
                     /locus_tag="PLB01_07985"
     CDS             complement(5435..6406)
                     /locus_tag="PLB01_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ77281.1"
                     /translation="MPRAAKVLLALVAVLLVLGAAAGVVFWKRPLATLAWIGRRRLRA
                     SGFEKHVRPSAAGPQVVFSGGGGAPVVLVHGAGDQAGGWSKVAPRLARSFSVVAVDLA
                     GHGESAPERGPLPFATLLAGFDGAMGTLPQGKVTLVGNSLGAWIATVWATRHPDRVSH
                     LVLVNGGPLLGDRPDLTLQPKTRAEADRTLAALRDPSAPLVPDFVVDDVIREAATGPI
                     ARIAAEAKGMSAYLLDGKLSGLDVPVDLLWGASDKLLSVDYAKRLAGQLPAARLTTIE
                     RCGHVPAVECPDRFTEALLKILKDPLPEPTVSSPPVAAPAAAAAPKP"
     gene            complement(6420..7415)
                     /locus_tag="PLB01_07990"
     CDS             complement(6420..7415)
                     /locus_tag="PLB01_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HQQ77282.1"
                     /translation="MSRSARIVSTGSYLPEIEVSNDALRARFDAGTPEFVDKMEAATG
                     IKTRWYAPPDWATSDLAVRAARKALDAAGKTPADVDLILLGTDSPDFITPATSVVIQH
                     KLGAKNAGTFDVGCACASFPTCLATAAGLIATNKSLKTVVAIGAYMMHKLTDPDDPMI
                     FFFGDGAGAAVLEPSETPGFVASGFRADGSYNRNWAILSGGTDEPASLESVKAGRTTV
                     KLVERYPPELNNEGWPKLVRQMAADGGFAVSEIDMLILTQVRRPTIELVMQDLGLPME
                     KTHVVMDKWGYTGSASVAMAFDDARAQGKVKTGDLVVFVASGVGYNMAGAAFRMV"
     gene            complement(7412..8176)
                     /gene="fabG"
                     /locus_tag="PLB01_07995"
     CDS             complement(7412..8176)
                     /gene="fabG"
                     /locus_tag="PLB01_07995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQQ77283.1"
                     /translation="MRVAIVTGGAAGIGRATAVRFAQGGCAVSAWDVDEAKAAALVPE
                     LQAAGAPAAEARKVDVADPAGVAAAVGEVLSRFGRIDVLVNNAGIVKDSQLVKWKDGA
                     VVSTMSDGDFDKVVSVNLKGVFNCTRAVAPAMIRQGSGVVLNASSVVGLYGNFGQTNY
                     AATKAGVISFTQTWARELGKFGIRVNAVAPGFVATEIIRAMPEKVISGMVAKTPVGRM
                     GRPEDIAEAYFWLASDAAAFVHGATLSVDGGLVLGT"
     gene            complement(8179..10962)
                     /locus_tag="PLB01_08000"
     CDS             complement(8179..10962)
                     /locus_tag="PLB01_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ77284.1"
                     /translation="MPRGRRPFLLSKNLLPLLVPVLLLAAPRVSAQTAAATLEVVATD
                     AAGAPVPGASVEAKSSGTGLTRTGVTGVTGIAKFAALPTGTYDVTVKLQGFETVAQKG
                     VVLRVGQTGRVNVTLAQRKAAEVSVVASAPLVDVYKIDSSTNVSPEQIRDLPTPDRDF
                     QNLAFIAPTVERERGAFRFVTGGPVIGGGGNASQSTILVDGVDFTDPALGLAKTKFSQ
                     DAISEFRVISNRFDAEVGGSAGGALSVLTKSGTNTLSGTAFGFYRGPNLRARGAFEQD
                     STVDYERNQFGATVGGPIVKDRTFFFGSIEQVNATTPTLFRPKGAFTSQAADYKVPIH
                     QTLGYLGLDQTLSENNHLIAKLDYERFRQDNFRVGGVAAIDYGQELNRDNLTFAVGDT
                     WNLGNGSVNEARAQYGSRKYEEPTNTARMAEWYSSGNTLQTGGNILGNLLGDGTQFEF
                     RDTLYLHFTGAGSHDVKVGAGVQRVIDRSRITTYEYGLMLYLTDSKALPLAYAYGIGS
                     ADVKANTTRLAAYVQDDWRPVSNLTVSLGVRYDYDSGGNNPNIYQPNLGLDGRPIDTN
                     NFQPRLGISWDITGKGQYVARGGVGMFTGRYLLVPAFTEMQQNGYTGRVTYTNINGAL
                     LGFPAFALDPNNPQNTGIKSKPAVTVIDKTLDAPESTQATLGFTAKLGQTGLYADVEG
                     IYMKGRKEITVTDRNWSGNASHTRPNTAFDQINTYTNDGRSEYMAAVFSLNGNIKGGH
                     VVTASLTLASKHNISDDFSPEFPTGYPNDPANLEAEYGRARSWERYRVVISGIARLPW
                     GINVAPVFEYGAGQPWTQRLGYDYNGDGKNSDRPAGVSRFAEDGPAYRNVNIRISKMF
                     NVSGIGVELIAECFNLFNTVNYDVTSIDGARYLSGPTIANPNAPANPNPNYGAASATL
                     SPREGQIGLRVSF"
     gene            complement(11093..12946)
                     /locus_tag="PLB01_08005"
     CDS             complement(11093..12946)
                     /locus_tag="PLB01_08005"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77285.1"
                     /translation="MICQVCGTGNELDREFCAKCQSKLLVVSGAAETYDEGTPEEGVS
                     LDEHLLERVSVLEEIVKRSAETLKMLLEALNRQEKNGFVSQTGLLALKDLLERKGLLV
                     EEELLDLWETRVDLHMAAMEKRERFLERKERMLGSFSGERRERFKGLLADAEFAFYAL
                     DPEKAVRSLEDAFRLDKTNAELAFYLGETAFNEGETERAAQLLHTVLAREPRHFEALV
                     YTGVLENEAGRTDRALEALKEAVAVKPDAFLPYFALGALFALRGQLARAETFLRKAVE
                     IDENPQAYSLLGTIAYERGRLAEATEAFQKAVRLDPDDEDALYQLGLCYLDRGWTQKA
                     AERFQSALELNPNRIEYQEACKLLAPSGARALPKVTGEAAQLARRAEAAAPRDPQKAL
                     AIYRRALKIDAENTTLLIAFALLCSAVGRTSEAVATTRRVLSREPGEMVAAAAYATLL
                     EALKAEGKFREGSRVAEEMLGAVRSNYARSIAYFQRATALAEMGEDLDAALELADLSL
                     KLSPKEMRQFPLAAKGWVHYKRREFDRAVECLRRATDLGETPTGLTHLGLAYLALGDA
                     RAARQAFGRAKRRDVRVSGGRGGLEAKMLEQIRRNLQLTEKLANRKKTRLA"
     gene            complement(12992..13873)
                     /locus_tag="PLB01_08010"
     CDS             complement(12992..13873)
                     /locus_tag="PLB01_08010"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HQQ77286.1"
                     /translation="MSLEKIGVVGAGQMGAGIAHVAALAGKSVVLLDVSDDAVQKGLK
                     AIGKNLGRQVEKGKVTADTRDAALARISPTTDLGRFADADLVVEAVVENEDVKKDLFG
                     KLDVLVKAGGILASNTSSISITRLAASTKRPERFIGMHFMNPVPVMTLVEVIRGIATS
                     DETTASVVALAEAMGKTPLLCRDFPGFVSNRVLMPMLNEAFFVLHEGIATPEAVDGIM
                     KLGMNHPMGPLTLADFIGLDTCLAILRVLHGGLGDDKYRPCPLLVQMVDAGWLGKKAG
                     RGFYRYDAPGGNRIEIS"
     gene            complement(13920..14888)
                     /locus_tag="PLB01_08015"
     CDS             complement(13920..14888)
                     /locus_tag="PLB01_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77287.1"
                     /translation="MRRKPRALLFLVAALLIASSPARPEEISASEIRVSAGTTRSGDL
                     VALGRRILLEGELEGSVVLVGGGEAVVTGRIRRDLVLLGANATIRRGARIDGDVLSLG
                     GSLTFEENPPVSSDVSPFRGPSHPQVGGRVRTVSALEAAVATELQTSPAASLAQWPFL
                     LSFRLALLFAWLVVSLLLLVLAPRAIGESAAGVAGRSAFLGALGATAVLTAALAGALA
                     LSLLPSRVSLAAGAVVLLALVAAKVWGLTALFLALGRRLNASARRGSALFGDPAAAAL
                     GLLALGAASLVPVVGPLVWGAASLVGIGVSLADAASRAPRVALAPS"
BASE COUNT         2272 a         5189 c         5213 g         2253 t
ORIGIN      
        1 ggccgccgag ggtcggggtc tcttccgccg ccatggccag gagtctgcgc gcggtctccg
       61 cggaccgtca agtgctttac agggcgaagc ggcaccggcg cgcaatcgca tacactcgac
      121 gcgatggacg tcacctcctt ctttcagacg ccggaaaagg ccgacgcggg cctctcccct
      181 ctcgcccgcg gcgtcgtcgg ctccgagatc ctccgcatcg cgggcgagat ccgcgcgctc
      241 caggccacgg gccgcccggt ctgcaacctc acgatcggcg acttcgactc gcgccagttc
      301 cccatcccgg acgggctcct cgccgcaacg aaggacgccc tcgagacggg ccagacgaac
      361 tacccgcctg cggaaggcat cctgccgctg cgcgaggagg tcgtgaagct ctacgcccgc
      421 gacctcgggc tctcgtaccc cgtcgagtcc gtcgtgatcg cggccggcgc ccggccgctc
      481 ctctacggca cctaccgcac gctcctcgag ccgggcgacg gcgcgatcta tcccgtgccg
      541 tcgtggaaca acaaccacta cgcgtacctg tccggcgccc gcgggatccc gatccgcgtc
      601 gaggcgaagt cgaacttctt tccgacgccg gaccaggtcc gccctcacgt ccccggcgca
      661 cggctgctga tcctgaactc cccgctcaac cccacgggca cggcgatctc gaagcaggcc
      721 ctgacggacc tcacggagat ggtggtcgag gagaatcgcc ggcgcgactc ggagggccgg
      781 aggcggccgc tctacctcgt gtacgaccag gtctactgga ccctcgtctt cgggaagacc
      841 gagcatcaca cgcccgtcgg gctcgttccc gagagcgcgc cgtacgtcat cttcctcgac
      901 gcgatctcga aggcgttctg cgcgaccggc atgcgcgtcg ggtggggcgt catgcccccg
      961 ccgatccgcc gccggatgtc cgacatcctc ggccacgtcg gcgcctgggc gccgaaggct
     1021 gagcaggtcg gcgtcgcgaa gttcctcgcg cagccggagg ccgtccgcgc gtatcgcgac
     1081 ggcgtgcgga agaagctcgc cgagcggctg gaggccctgt acgacggcgt gatgtccatg
     1141 aagaaggagg gcttccccgt cgacgcgatc gagccccagg gggcgatcta cctctccgtg
     1201 cgcttcgacc tcttcgggcg cacggtgagg ggagtccccg tgaagacgaa cgacgacgtg
     1261 cggcgcctcc tcctcgaaga ggccggcctc ggcgtcgtgc cgttccaggc cttcgatttc
     1321 gagggcgaca cgggctggtt ccggctgtcc gtgggcgcgg tctccatgga cgagatcgcg
     1381 gccgcgttcc cgcgcctccg ggcgatgctc gcggaagcgc ccgccgccgt ctgacgggat
     1441 ccgccgcggc attcgtctag actcgaagcg tggagcagga caagaagtcc gagaggagcc
     1501 gcaaggccat cctcgaggcc gcgctggacc tcttctcgag ccaggggtac cgcgggacga
     1561 gcattcgcga catcgcggag cgcgcgggag tctccacggg gaacgtctac caccacttcg
     1621 ccgacaagga gacgatcttc cagacggtcc tcggccagta ctggaaggcg atcgagagcc
     1681 cggacttccc gttcaacaag gcgctcgcgt ccggaacgtt ccccgacaac ctcgaggagc
     1741 tggccgcagc cgccaaggag agcgtccgcg tctggagacg ccacgtcgcg ctcatctacg
     1801 tcgacgtcgt ggagctctcc ggaaaccaca tccgcaagtt ctactccgag atggccgtgc
     1861 gcttcgagcg gttcctcgtg tcgcacggcg actccgtctc cggcgagatc cggccggggg
     1921 tgccggtccc gaccgcggtc atgctcgcga gccgcgtctt cctccagtac tacgcggtcg
     1981 agatcctctt cggcgtcccg gtgcagttcg gcaaggacag cgacctcgtg atccgcgaga
     2041 tcgccgagat cctgcggcac ggggtcctga aagaggaacg gctcgcggcg tccgcccccg
     2101 tccctctctc ccgcgcgcgc cgctagaccg cctcggtccc cgtcaggaac cgccgcacgc
     2161 gcggccagaa cacctcgggc ttctcgaggt acacgacgtg cccggcgccc ggaatctcct
     2221 cgtagacggc atgcgggatc acggtcggga ttctccgctg ttggggcggc ggaatcgcac
     2281 ggtcctcggc gcccgcgagg acgaggaccc ggcacgggat ggccgcgtag gactccgcgt
     2341 tctccccgag cgcggcgaag aacgggtcct gcgccttcgt gagccgcacg agcgcgtcga
     2401 tgcgccccgc gtaccggtcg tggaagccct ggcgcatgga ctcgagcttc ggtccggcgc
     2461 gcgcgacgaa cgcctccgcg aagatcttct cgtagaggta ccgggtgtag aggtcgaaga
     2521 ggggcggccc gcccgcgtag aaccgcaggg agacgtcctg gtacatcgcg aagagcgtct
     2581 cctgcgtcag gaggatgccg gagagcgtca acgacgccgc gcgcgccggg tggcgccgcg
     2641 cgaactccgc ggcgacgaac ccgccgtaag aaacaccgat cgcgtggatc cgggagatcc
     2701 cctcggcgtc caggatcgcc gcgagcgcgt ctccgaagga cgagatcggc gcgcccggca
     2761 gctccgcgtc gtccgacgcg ccctgcccga ggtagtcgta caggaggacg tcgcgctccc
     2821 ccgtcagctc gggcaggaag gacagccagc tcttcgtgga catcgcgagc ccgttgaaga
     2881 ggcagacggt ctcgcggccg ccggccccgt agagctccca gtggtagccg tgcccgccgt
     2941 ggacgagccg gccctcgcgg agaggcgctg gggggggggc cgcgctcacg ccgctcctcc
     3001 ggagcggtgc acgcgcgcga ggaaccgctc cacgaggagg acgaactcgt cctcgcgttc
     3061 gacgacgaga gcatggcccg cgccgggcac gacgacgaaa tgcgcgaccg ggattccgtc
     3121 cgccacggct ttcgtccgtt cgcgcggcat cgcggcgtcg cgttccgcgg cgacgacgag
     3181 aaccggacag gagatcctcc cgagggccgg cctcagatcg accttgtcga gtgccgctag
     3241 gagaccctcc agtcccgtga accacgtctc gggcaggagg ccgacctggc cgcggcgcgc
     3301 cgcgagctcg tccgcgtggg cgacgcgcca ctcgggggtg tacgcgagct ccacgatcgc
     3361 gtcgtaggcc tccgtccggt cgcctcctcg agccgcggcg cgaaccgccg ccgccaggcg
     3421 agcgccggcc tcgccgagcg cggcgtctcc ttccctcgcg acgtcggtca ccgtcgcggc
     3481 gacgaccgac cggacgcgtt cggggcggcg cgccgcgaga aggagccccg cgtatgcgcc
     3541 gtacgacgcg cacaccacgt cgacgggtcc gaccgaaagg gattcgagga gcgccgcgac
     3601 gtcgtccgcg tgctcttcca tggacgcgcg cggagggccc ggcgagagga gctggccccg
     3661 gaagtcgcag cggatgacgc ggtgccgctc ggcgaagcgc cgcgcgatcg gctcccacgc
     3721 cgagagcgac atcatcccgc cgttgaggag caagagaggg gggccctcgc cgtccacccg
     3781 atgtgtcaag agcggctggc tcatgctcat cgggcttcat tcgttgaaga tttctttgaa
     3841 aggtaaagag agcggaggtc acccttcacg actttcccat agggggtccg gggcaggctc
     3901 gcgacgaaga tgaactcccg gggaaccttg tacttcgcga ggcgcgcctc gacaaaggcc
     3961 gccagttcgg ccatctctcc tattaccttc aaagaaatct tctcttctcc cttctcctct
     4021 ctcccttctt ccctccgaac gacgaaggcc acgccgacct cgccccactt ctcgtcgggc
     4081 acgccgacga ccgcgacgtc cttcacccgc ggatgctgga agaggacacc ctcgatctcg
     4141 gccgggtaca cgttcacgcc gcccgagatc agcatgtcct tcttgcggcc cgcgatcgtg
     4201 aagaacccgt cctcgtcctt cttcgcgagg tcgcccgtgt ggaaccagcc ctcggcatcg
     4261 agcgacgccg ccgtcgcctc gggattccgg aagtagcccc ggcagacgtg cgggcccctg
     4321 aggaggagct cgccgacctc gcccaccggg acctcccgcc cgtcctcgcc ggtcagcttc
     4381 gcctccgtga acatcatcgg cctgccgatc gagccggcct tccgcacgga ctcctcgacg
     4441 ctcatcgcga agcagttcac gccgacttcg gtgaggccgt agccctgctt gaagttcgcg
     4501 ccgcgccggt gccacgtctc gatgatgtcg agcggcagcg gcgcaccacc gctgatgaag
     4561 tagcggaccg aggagaagtc ggccgacgcc cacgacgggt gctcctggag catccggaag
     4621 atcgtcggca cgccgaggac caccgtgcag cgctccttcc cgatcgtcgc gaggacctcc
     4681 gcggcgtcga agccggcgtg gaggacgatc gtcccgccga tcgcgaagac cggcgtcagg
     4741 aacgcgccga ggccgcccgc gtggtacagc ggcgtgtaga tcggcgacac gtcgtcctcg
     4801 cgcagctgcc acgacacgac ggtgttcacc gcgttccacg cgaccatccg gtgcggcacc
     4861 atcacgccct tcggcttgcc cgtggtgccg gaggtgtaga ggaggcagta gagatcttcc
     4921 gggccgggag aggggacttc tgcagcggcg gcaggagccg ccgctggagg agaagagaag
     4981 atttccttga aggtggtgcg ggcgttcggc gcgcccgccg ccctcgcgcc acgctgcgcc
     5041 ggcgcgtcga gaagaaccgg ggttacccct cttacctttc taagcaaatc ttcaacggtc
     5101 tcttcgaacg cggctccata gatcagcgct ttcggctctg cgtcctcgag gacgccaatc
     5161 agctctcccg ccgtctggcg cgtgttgagc gggacgagga cgacgccgtt cttcccgcac
     5221 gcgaagaaga cgtcgaggaa ctccacgcaa ttgcccgcga ggagggccat gcggtcgccc
     5281 tttttcaggc cgagcccctc ggtcagcgtc cgtgccgcgc ctctcgcgcg cgcatccagc
     5341 tccgcatagg tgaagcgccg tcccgtcgcg acctcgacga gcgcggtctt ctccggcgac
     5401 agccgcgcgc gctccccgag gacgtcgccg gagatcacgg cttcggcgcc gccgcggcgg
     5461 cgggcgcggc gaccggcggc gaggagacgg tcggttcggg cagagggtcc ttgaggatct
     5521 tcaggagcgc ctccgtgaag cggtcggggc actcgacggc gggcacgtga ccgcagcgct
     5581 cgatcgtcgt cagacgcgcg gccgggagct ggccggcgag gcgcttcgcg tagtcgaccg
     5641 acagcagctt gtccgacgcc ccccagagga ggtccaccgg gacgtcgagc cccgagagct
     5701 tgccgtcgag caggtacgcg gacatgccct tcgcctccgc ggcgatccgc gcgatcggcc
     5761 ccgtggcggc ctcgcggatg acgtcgtcga ccacgaagtc cgggaccagc ggggccgacg
     5821 ggtcgcgcag agccgcgaga gtccggtccg cctccgcgcg cgtcttcggc tggagcgtga
     5881 ggtccgggcg gtcgccgagg agcggcccgc cgttcacgag caccaggtga ctcacccggt
     5941 cgggatgccg cgtcgcccag accgtcgcaa tccacgcacc gagggagttc ccgacgagag
     6001 tgactttccc ctgcgggagc gtccccatgg cgccgtcgaa tccggcgagg agcgtcgcga
     6061 acggcagcgg acccctctcc ggcgcgcttt cgccgtgccc ggcgaggtcc acggccacga
     6121 ccgagaagct cctcgcgagg cgcggagcca ccttcgacca gccgccggcc tggtctccgg
     6181 cgccgtggac gagaacgacg ggcgctccgc cgccgccgga gaacacgacc tgcggcccgg
     6241 ccgcggaagg ccggacgtgc ttctcgaagc cggacgcccg gaggcgccgg cggccgatcc
     6301 acgcgagcgt cgcgaggggc ctcttccaga agaccactcc cgcggccgcc ccgaggacga
     6361 ggaggacggc gacgagggca aggaggacct tcgcggcgcg cggcacggtg cctcgcagac
     6421 tagaccatgc ggaaggcggc gcccgccatg ttgtagccga cgccggacgc cacgaagacg
     6481 acgaggtcgc cggtcttgac cttgccttgc gcacgggcgt cgtcgaacgc catcgcgacg
     6541 ctcgccgagc ccgtgtagcc ccacttgtcc atcacgacgt gggttttttc catcggcagg
     6601 ccgaggtcct gcatgacgag ctcgatcgtc ggccgcctga cctgcgtcag gatgagcatg
     6661 tcgatctcgg agacggcgaa cccgccgtcc gcggccatct ggcgcacgag cttcggccag
     6721 ccctcgttgt tgagctcggg cgggtagcgc tcgacgagct tgaccgtcgt gcggccggcc
     6781 ttcacggact cgagcgaggc cggctcgtcg gtgccgcccg acaggatcgc ccagttgcgg
     6841 ttgtacgacc cgtccgcgcg gaaccccgac gcgacgaagc cgggcgtctc ggaaggctcg
     6901 agcacggccg cgcccgctcc gtccccgaag aagaagatca tcgggtcgtc cgggtcggtg
     6961 agcttgtgca tcatgtatgc cccgatcgcg acgaccgtct tgagcgactt gttcgtcgcg
     7021 atgaggccgg ccgcggtcgc aagacacgtc gggaacgagg cgcacgcgca gccgacgtcg
     7081 aacgtccccg cgttcttcgc cccgagcttg tgctggatga cgacggacgt cgcgggcgtg
     7141 atgaagtccg gcgagtccgt cccgaggagg atcaggtcga cgtccgcggg cgtcttcccg
     7201 gccgcgtcga gagcctttcg ggcggcgcgc acggcgagat cggacgtcgc ccagtcgggc
     7261 ggcgcatacc agcgggtctt gatgccggtc gcggcctcca tcttgtcgac gaactccggc
     7321 gtgcccgcgt cgaagcgcgc ccggagcgca tcgttcgaca cctcgatctc cggcaggtag
     7381 gagccggtcg agacgatccg ggccgaccgg ctcatgttcc gagcaccagc cccccgtcca
     7441 cggagagcgt cgcgccgtgg acgaacgcag ccgcgtccga cgcgagccag aaatacgcct
     7501 cggcgatgtc ctcgggcctg cccatccggc cgacgggagt cttcgcgacc atgcccgaga
     7561 tgaccttctc gggcatcgca cggatgatct ccgtcgcgac gaaacccggc gcgaccgcgt
     7621 tcacgcggat gccgaacttg ccgagctcgc gggcccacgt ctgggtgaac gagatgaccc
     7681 ccgccttcgt cgcggcgtag ttcgtctggc cgaagttgcc gtagaggccc acgacggacg
     7741 acgcgttcag gacgacgccg ctcccctgcc tgatcatcgc cggggccacg gcgcgcgtgc
     7801 agttgaagac gcccttgagg ttgacggaga cgaccttgtc gaagtcgccg tccgacatcg
     7861 tcgagacgac ggcgccgtcc ttccacttca cgagctggga gtccttcacg atcccggcgt
     7921 tgttcacgag aacgtcgatc cgcccgaagc gagagaggac ctctccgacg gccgccgcga
     7981 cgcccgcagg atccgcgacg tccaccttcc gggcctcggc ggcgggcgcg cccgccgcct
     8041 ggagctcggg aacgagagcc gcggctttcg cctcgtccac gtcccatgcg gacacggcgc
     8101 agccgccctg ggcgaaccgc acggccgtgg cgcggccgat gccggcggcg cctcccgtca
     8161 cgatcgcgac gcgcatgttc agaacgacac cctcaggccg atctggccct cgcgcggcga
     8221 cagcgtcgcg gaggccgcgc cgtagttcgg attcgggttc gcgggggcat tggggttcgc
     8281 gatcgtcggt cccgacaggt accgggcgcc gtcgatcgag gtcacgtcgt agttgaccgt
     8341 gttgaagagg ttgaagcact cggcgatcag ctcgacgccg atgccgctga cgttgaacat
     8401 cttcgagatg cggatgttca cgttgcggta ggccgggccg tcctcggcga agcgcgagac
     8461 gcccgccggg cggtccgagt tctttccgtc accgttgtag tcgtagccga gacgctgcgt
     8521 ccacggctgg ccggcgccgt actcgaagac cggggcgacg ttgatgcccc acggcagccg
     8581 cgcgatgccc gagatgacga cgcggtaccg ctcccaggag cgggcgcggc cgtactcggc
     8641 ctccagattg gccgggtcgt tcgggtagcc cgtcgggaac tcggggctga agtcgtcgga
     8701 gatgttgtgc ttggacgcga gcgtgagcga cgccgtcacg acgtgcccgc ccttgatgtt
     8761 gccgttcagg gagaacacgg cggccatgta ctcggagcgg ccgtcgttcg tgtacgtgtt
     8821 gatctggtcg aacgccgtgt tcggccgggt gtgcgacgcg ttgccgctcc agttgcggtc
     8881 ggtgaccgtg atctccttgc gtcccttcat gtagatgccc tcgacgtccg cgtagaggcc
     8941 cgtctgcccg agcttcgccg tgaagccgag cgtcgcctgc gtggactcgg gcgcatcgag
     9001 cgtcttgtcg atgaccgtga cggcgggctt ggacttgatg cccgtgttct gcgggttgtt
     9061 cgggtcgagg gcgaaggccg ggaacccgag gagcgcgccg ttgatgttcg tgtaggtcac
     9121 gcggcccgtg tagccgttct gctgcatctc ggtgaacgcc gggacgagga ggtagcgtcc
     9181 cgtgaacatc ccgacgccgc cgcgggcgac gtactggccc ttccccgtga tgtcccacga
     9241 gatgccgagg cggggctgga agttgttcgt gtcgatcggg cggccgtcga ggccgaggtt
     9301 cggctggtag atgttcgggt tgttgccgcc cgagtcgtag tcgtagcgca cgccgaggct
     9361 caccgtgagg ttcgagacgg ggcgccagtc gtcctgcacg taggcggcga gacgcgtcgt
     9421 gttcgccttc acgtcggccg agccgatgcc gtaagcgtac gcgagcggga gggccttcga
     9481 gtccgtgagg tagagcatga ggccgtactc gtaggtcgtg atccgcgagc ggtcgatcac
     9541 gcgctggacg cccgcgccga ccttcacgtc gtgcgagccc gcgcccgtga aatggaggta
     9601 aagcgtgtcg cggaactcga actgcgtgcc gtcgccgagg aggttgccga gaatgttgcc
     9661 gccggtctgg agcgtgttgc cgctcgagta ccactcggcc atcctcgcgg tgttcgtggg
     9721 ttcctcgtac ttgcgcgagc cgtactgggc gcgcgcctcg ttcacggagc cgttgccgag
     9781 gttccacgtg tcgccgacgg cgaacgtcag gttgtcgcgg ttcagctcct ggccgtaatc
     9841 aatggcggcg acgccgccga cgcggaagtt gtcctggcgg aagcgctcgt agtcgagctt
     9901 cgcgatgagg tggttgttct cggaaagcgt ctggtcgagg ccgaggtacc cgagcgtctg
     9961 gtggatgggg accttgtagt cggccgcctg ggacgtgaac gctcccttcg ggcggaagag
    10021 cgtgggcgtc gtcgcgttga cctgctcgat cgagccgaag aagaacgtcc ggtccttcac
    10081 gatcgggccg ccgacggtcg cgccgaactg gttgcgctcg tagtcgacgg tggagtcctg
    10141 ctcgaaggcg cccctggcgc gcaggttcgg gccgcggtag aagccgaagg cggttcccga
    10201 gagcgtgttc gtgccgctct tcgtcagaac cgagagcgcg ccgcccgcgg atccgccgac
    10261 ctcggcgtcg aaacggttgc tgatgacgcg gaactcggag atcgcgtcct gcgagaactt
    10321 cgtcttcgcg agcccgaggg cggggtccgt gaagtcgacg ccgtccacga ggatcgtcga
    10381 ctgactcgcg ttcccgcctc cgccgatgac ggggccgccc gtcacgaacc ggaaggcgcc
    10441 gcgctcgcgc tcgacggtcg gcgcgatgaa cgcgaggttc tggaagtcgc ggtcgggcgt
    10501 cgggagatcc cggatctgct ccggcgagac gttcgtcgag gagtcgatct tgtaaacgtc
    10561 gacgagcggc gccgaggcga cgacgctcac ctcggcggcc ttccgctggg cgagcgtgac
    10621 gttcacgcgt cccgtctgcc cgacgcgaag gacgacgccc ttctgcgcga cggtctcgaa
    10681 tccctgcagc ttcacggtca cgtcgtacgt gcccgtgggc agcgcggcga acttcgcgat
    10741 gcccgtcacg cccgtgacgc cggttcgggt gagccccgtg ccggagctct tcgcctcgac
    10801 gctggcgccc ggcaccggcg cgcccgcggc gtccgtcgcg acgacctcga gtgtcgcggc
    10861 ggccgtctgc gccgagacgc gcggcgccgc gaggaggagg accggaacaa ggagaggaag
    10921 aagattcttc gaaagcagga aggggcggcg gcctctgggc atcgtttcct cctgcggcac
    10981 ccgccgcaaa accggacaat cggtttgttt tatgaactct atattcaaag aaaactccgt
    11041 gtcaacaacg gaaacgcatg ccaagaacga cttgcgttaa cgcaagtgag tctcaggcca
    11101 ggcgcgtctt cttccggttc gcgagcttct cggtgagctg gaggttccgg cggatctgct
    11161 cgagcatctt tgcctcgagc ccgccgcgtc cgcccgagac gcgcacgtca cgacgcttgg
    11221 cgcgcccgaa ggcctgccgc gccgcacggg cgtcgccgag cgcgaggtac gcgaggccga
    11281 ggtgcgtgag gcccgtcggc gtttcgccga ggtccgtcgc gcgcctcagg cactccacgg
    11341 cccggtcgaa ctcacgccgc ttgtagtgca cccagccctt ggccgcgagc gggaactgcc
    11401 gcatttcctt cggcgagagc ttcagcgaga ggtccgcgag ctcgagggcg gcgtccaggt
    11461 cctcccccat ctccgcgagc gccgtcgcgc gctggaagta cgcgatcgag cgcgcgtagt
    11521 tggagcgcac cgccccgagc atctcctcgg cgactcggct cccctcgcgg aacttccctt
    11581 cggccttgag cgcctcgagg agcgtcgcgt acgcggccgc cgccaccatc tccccgggct
    11641 cgcgcgagag gacgcggcgc gtcgtcgcga cggcctccga cgtccggccc acggccgagc
    11701 agaggagcgc gaacgcgatc aggagcgtcg tgttctccgc gtcgatcttc agcgcgcgcc
    11761 ggtagatcgc gagcgccttc tgcgggtcgc gcggagccgc ggcctccgcc cggcgcgcga
    11821 gctgcgcagc ctcgccggtc accttcggaa gcgcccgcgc gcccgacggt gcgaggagct
    11881 tgcaggcctc ctggtactcg atgcggttcg ggttcagctc gagcgcggac tggaagcgct
    11941 cggcggcctt ctgcgtccac ccgcggtcga ggtagcagag cccgagctgg tacagcgcgt
    12001 cctcgtcgtc cgggtcgaga cgcacggcct tctggaacgc ctccgtcgcc tccgcgaggc
    12061 ggccgcgctc gtacgcgatc gtgccgagca gggagtacgc ctgcgggttc tcgtcgatct
    12121 cgacggcctt ccggaggaac gtctcggcgc gcgcgagctg cccgcggagc gcaaagagcg
    12181 caccgagcgc gaagtacggg agaaacgcgt ccggcttgac cgcgacggct tccttgagcg
    12241 cctcgagcgc gcggtccgtc cggccggcct cgttctcgag gacccccgta tagacgagcg
    12301 cctcgaagtg gcgcggctcg cgcgcgagga ccgtgtggag gagctgcgcg gcgcgctcgg
    12361 tctcgccctc gttgaacgcg gtttcgccga ggtagaacgc gagctccgcg ttcgtcttgt
    12421 cgaggcggaa cgcgtcctcg agcgaacgca cggccttctc ggggtcgagt gcgtagaagg
    12481 cgaactcggc gtccgcgagg agccccttga accgctcgcg ccgctcgccc gagaagctgc
    12541 cgagcatccg ctccttgcgc tcgaggaagc gctcgcgctt ctccatcgcg gccatgtgga
    12601 ggtcgacgcg cgtctcccag aggtcgagga gctcctcctc gacgaggagg cccttgcgct
    12661 cgagaaggtc cttgagggcg aggaggcccg tctgcgaaac gaaaccgttc ttctcctgcc
    12721 ggttcagcgc ctcgagcagc atcttgagcg tctcggcgct gcgcttgacg atctcctcga
    12781 ggaccgagac gcgctcgagg aggtgctcgt ccagcgagac gccttcctcg ggcgtgccct
    12841 cgtcgtaggt ctctgccgcg cccgagacga cgaggagctt gctctggcac ttcgcgcaga
    12901 actcgcggtc gagctcgttg cccgttccac acacctgaca gatcacggga cgaccacccg
    12961 aggagctccc gccgctcgcc cctcttccct tctaagaaat ctcaatccga ttcccaccgg
    13021 gggcgtcgta acgatagaag cctcggccgg ccttcttccc gagccagccc gcgtcgacca
    13081 tctgcacgag gagcgggcac gggcggtact tgtcgtcgcc gaggccgccg tgcaggacgc
    13141 ggaggatcgc gaggcacgtg tcgaggccga tgaagtcggc gagcgtgagc gggcccatcg
    13201 ggtggttcat gccgagcttc atgatcccgt ccacggcctc gggcgtcgcg atgccctcgt
    13261 ggaggacgaa gaacgcctcg ttgagcatcg gcatcaggac gcggttcgag acgaagcccg
    13321 ggaagtcgcg gcagaggaga ggtgtcttcc ccatcgcctc ggcgagggcg accacggagg
    13381 ccgtcgtctc gtcggacgtc gcgatccccc ggatgacctc gacgagcgtc atgacgggca
    13441 ccgggttcat gaagtgcatt ccgatgaagc gctccggccg cttcgtcgag gccgcgagac
    13501 gcgtgatcga gatcgacgag gtgttggagg cgaggatgcc gccggccttc acgaggacgt
    13561 cgagcttccc gaagaggtcc ttcttgacgt cctcgttctc gacgacggcc tcgacgacga
    13621 ggtccgcgtc ggcgaaacgc cccaggtccg tcgtgggaga gatgcgcgcg agcgcggcgt
    13681 cgcgggtgtc cgccgtgacc ttgcccttct cgacctgccg gccgaggttc ttcccgatcg
    13741 ccttcaggcc cttctgcacg gcgtcgtcgg agacgtcgag gaggaccacg ctcttgccgg
    13801 cgagcgccgc gacgtgcgcg attcccgctc ccatctggcc ggcgccgacg acgccgatct
    13861 tttcgagact catccgctgg actcctgtgg ggccgcccgg cgacccggcg ccgagcctat
    13921 caggagggcg cgagcgcgac ccgcggggcc cgggaggcgg cgtcggcgag ggaaacgccg
    13981 atcccgacga gcgacgccgc gccccagacg aggggaccca cgaccggaac gagcgatgcg
    14041 gcgccgagag cgaggaggcc gagtgcagcc gcggccggat cgccgaagag cgcgctgccg
    14101 cgccgggcgc tcgcgttcag gcggcggccc agggcgagga agagcgccgt gagcccccag
    14161 accttcgccg cgacgagcgc caggaggacg accgcgccgg ccgcgagcga aacgcgcgac
    14221 ggcaggagcg acagcgcgag cgcgccggcg agcgcggcgg tcaggacggc ggttgcaccg
    14281 agcgcgccga ggaacgcact ccgcccggcg actcccgctg cggactcgcc gatcgcccgc
    14341 ggcgcgagaa cgaggaggag gagcgagacg acgagccacg cgaagaggag cgcgaggcgg
    14401 aacgagagga ggaacggcca ctgcgcgagc gatgcggcgg gggacgtctg cagctccgtg
    14461 gcgacggcgg cctcgagggc ggagaccgtg cgaacgcgcc cgccgacctg cggatgggac
    14521 ggcccgcgaa aaggcgagac gtccgaagag accgggggat tttcttcgaa ggtgagagag
    14581 ccgccgaggg agaggacgtc gccgtcgatg cgggctccgc ggcgaatcgt ggcgttcgca
    14641 ccgaggagaa cgaggtcacg acgaatgcga cccgtcacca cggcctcacc gcctccgacg
    14701 agcaccacgc ttccttccaa ttcaccttct aagagaatcc tccttccgag agccacgagg
    14761 tcgccggagc gggtcgtccc ggccgacacg cggatttccg aagcagaaat ctcctccggc
    14821 cgtgccggag acgacgcgat gagcagtgcg gcgacgagaa agagaagggc gcgcggcttg
    14881 cggcgcatgg cgccgcgtca gccgcgcgcg gcgggcgccg cggcgcg
//
LOCUS       DAOMFY010000074        14926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1237_length_14926_cov_7.524982, whole genome shotgun sequence.
ACCESSION   DAOMFY010000074 DAOMFY010000000
VERSION     DAOMFY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1237_length_14926_cov_7.524982"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLB01_08020"
     CDS             complement(<1..639)
                     /locus_tag="PLB01_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQQ77288.1"
                     /translation="MRVSFVTCDVFTDRPFAGNPLLVVPDARGLTTAQMQSIAREINY
                     SESTFVLPAKDAAHAYWQRTFVPVKEIPYAGHPTVGTAVVMAALGRAAAGAPDGIHPV
                     TIEVGFGPLKLELLKKDGRVTRVRMEQGKPEWKAPVTGDDVKGQIAAALGVPFDALHP
                     ALPPQAVGTGNTFLMVPLASVEAVSSALADTRMLNHVERELGVLGLLFFAFDA"
     gene            complement(636..1352)
                     /locus_tag="PLB01_08025"
     CDS             complement(636..1352)
                     /locus_tag="PLB01_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ77289.1"
                     /translation="MTDAPGKFAPNADVYGPAYYGRMYRPHWFLRNRRKYEERDAALL
                     RIVRPSPSLRVLEVGSARGDTAFFLAPCVAEVVGIDAAGVAVDAARAGQAARGLSNVR
                     FERADARDLAAAPGGPFDVVLLADFVEHVLDDVLVPCLESARRSLVSGGALAIYTPNR
                     DHWAERIKAAVPGLQQEDHIAVRPATRVVSLVEEAGFRVEDLFYSASPYPVLGALDRA
                     LPMVGLCRFRTCLRAVTSSP"
     gene            1340..2761
                     /gene="trmFO"
                     /locus_tag="PLB01_08030"
     CDS             1340..2761
                     /gene="trmFO"
                     /locus_tag="PLB01_08030"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HQQ77290.1"
                     /translation="MHPSRDESTHLKREPAPRMDSMARVTVIGGGFAGVECAHQLLRR
                     GHAVTLVEMRPVRTTDAHATDRLAEMVCSNSFRSDNPANAVGLLKREMESVGSLILEE
                     ARRAAVPAGDALAVDRTIFAEAVTRRLAGEPLLTLVRGEAEALPDPGDGFVVVATGPL
                     TSPALEKALLGLLGEKYLYFYDAMAPIVEAASLDMAKLYALSRYGKGGGDDYLNVPLS
                     RAEYEAFVAALLAGEKVPFHDFEKAVYFEGCLPAEAMAERGVETLRHGPMKPFGLPDP
                     RTGREPYAVVQLRQDDLAKTHFNMVGFQTKLKVGEQRRVFRMLPGLENAEFVRYGMLH
                     RNTYINGPAHLDRFFRWRKDPRVFFAGQLTGVEGYLESAATGLMVGAALAQLLEGREP
                     APLAFSTALGSLARHVSTPREGDFSPMNVTFGLIDDADVPPSKDRAKRRGEIARRALE
                     GVARWKTEALPVPSVTVPGAGAA"
     gene            2783..3103
                     /locus_tag="PLB01_08035"
     CDS             2783..3103
                     /locus_tag="PLB01_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQQ77291.1"
                     /translation="MKKNLTAALTAVLFAAAPAAVADGRSENFQTYCSVCHGDDGKGQ
                     TEEGKKKGARDLTNAKWQDKVDDGRLIKSVTKGHDKMPAFEKKLSADEIKALVAEVRT
                     LAKK"
     gene            3103..4032
                     /gene="xerC"
                     /locus_tag="PLB01_08040"
     CDS             3103..4032
                     /gene="xerC"
                     /locus_tag="PLB01_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQQ77292.1"
                     /translation="MRGRLPGELAAFDRHLADERGVSRHTRAAYGKDLGRFAAFLSTV
                     FWNRPLEKIAAADVDALAVRSYLAHLRADGLTKASIGRHLSALRTFFAFLKREGHVVA
                     NPAKAIATPRKERSLPRTLSVTEAGAVVEAKGREGALGARDRALLEVLYATGLRVSEV
                     VGLKLEDVDLSARQVRTVGKGRKERIVPFGRAACDAVKTWLRARADMRPAAKDAGFLF
                     LNARAGRLTDRSVRRILDRAMLGADVTRHASPHALRHSFATHLLAAGADLRSIQELLG
                     HASLSTTQRYTHLDAERLLEVYRKSHPKAEDRE"
     gene            4059..4601
                     /gene="hslV"
                     /locus_tag="PLB01_08045"
     CDS             4059..4601
                     /gene="hslV"
                     /locus_tag="PLB01_08045"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQQ77293.1"
                     /translation="MAPPVFHHTTILCVRRGSSVALGGDGQVTMGTTVMKHGASKVRR
                     LLDGRVLAGFAGSTADAVTLFTRFEKKLEEFHGQLERAAVELATDWRTEKTLRQLEAL
                     LVVADAKVSLLLSGTGDVIAPDEGVLAVGSGGPYAAAAAKALLRHSTLEAAAIVRESL
                     AIAATIDIYTNDTIKVETLP"
     gene            4598..5944
                     /gene="hslU"
                     /locus_tag="PLB01_08050"
     CDS             4598..5944
                     /gene="hslU"
                     /locus_tag="PLB01_08050"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQQ77294.1"
                     /translation="MTPLTLRDLSPRKIVAELDRHVVGQAKAKRSVAIALRDRWRRQR
                     LPADLARDTTPKNILMIGPTGVGKTEIARRLARLVDAPFVKVEASKFTEVGYVGRDVD
                     SIARDLVDAAIRLVKEERRARVADRAAAAAEERLVDLLVAALATDPSGRAVAQEGRDK
                     VRRELHEGRHEAKEIEIEVADDRTPQISVMGPQGMEEMGIDIPGMIPGLFGGPKKKKV
                     RLPVSEARPLLLEREADALTDEATLPSEAIRRAEETGIVFLDEIDKIAGREGARGGPD
                     VSREGVQRDLLPLVEGTTVKTKHGMFKTDHVLFVAAGAFHVAKPSDLIPELQGRFPIR
                     VSLDALSEEDFLRILTEPEHALPKQSAALLGADGITLEFADDGLREIARAAAEVNRNL
                     ENIGARRLHTILETVLEEISFAGPDAAGARVVVDKAYVDRALASLVRDRDLSRFVL"
     gene            5941..7017
                     /locus_tag="PLB01_08055"
     CDS             5941..7017
                     /locus_tag="PLB01_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77295.1"
                     /translation="MRRALLLLAVAFFPGCGRKLAPEAPLLVLPARVEPVRISQEAGD
                     VVLRFPYPSVTNSGSTLTNLTKVTVAREIQSIREGQRIPPPPADAAQRESEERTFRAR
                     AETVAELSARDLDAATVGADLVFRDPLVPLYREKRLGRVLLRYAVTATRDKKRVSELS
                     PIASIVPRVPPDRPLRVIPAVEENRVCLDWWPPPAMLDGSKPVVIAGYAIYRRDASEE
                     EFEDQPIGVAIGATTYVDATTRPDRAYVYTVRAAPVADVPLILGPAADPVAVSTKDVF
                     PPPAPEGLLVLAESGGTRLVWNPSLAPDLAFYRVYRTDGGAPARLADGLKDPLFFDAG
                     ASGRTYGVTAVDKSGNESAPGTVK"
     gene            7014..7871
                     /gene="dapF"
                     /locus_tag="PLB01_08060"
     CDS             7014..7871
                     /gene="dapF"
                     /locus_tag="PLB01_08060"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQQ77296.1"
                     /translation="MKISPLGGLPIRFTKMSGAGNDFLVWGEAVSVGAAEVAAIQRLC
                     RRGTGVGADGVLFVFPDGPGRVSADYRNADGSVGRFCGNGTRCAARFAVLKGLAPENL
                     IVRTGWGDVPSRVDGTRVTLRLPEPVAVGRAVSSLDASGALEREAYALTVGVPNVVAF
                     VAGGMDVAALDFARIGPALRYHPELREGANADVAQVLGPSRLRVRTWERGVEAETLAC
                     GSGNVATAVTAAALGRVTPPVSLETRSGQVLRVDFRFEGEVARDVTLAGDARVVYEGT
                     LDPGEWEDA"
     gene            7868..8638
                     /locus_tag="PLB01_08065"
     CDS             7868..8638
                     /locus_tag="PLB01_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQQ77297.1"
                     /translation="MRFVRLVNGGAPAWALAQEETLVPLAGAPWETGGVPKPSGDAVP
                     RRGARLLPAASPTKILCIGRNYRAHAAELGHEVPKEPLVFFKPPSSLLPHGGTVRLPR
                     ESARVDYEGELALVIGRRARRVAKEDWRDVVFGVTCALDVTARDLQKTDGQWWRAKGF
                     DTFCPLGPAVETDVDPSTLTLQTFVDGAPRQNGNTADFIFDVGTLIAWTSAAMTLEPG
                     DVILTGTPEGVGPLAAGQTVEVRISGVGTLSVSVAAED"
     gene            complement(8635..10071)
                     /locus_tag="PLB01_08070"
     CDS             complement(8635..10071)
                     /locus_tag="PLB01_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQQ77298.1"
                     /translation="MSAAPRTFHLVAVGGTAMTPLASLLLARGERVTGSDGPVYPPMS
                     DRLAALGITVLPAFRAENVGADVSEVVAGNLARRDNPEILEALRRGLNVRSMPETLHD
                     EILVGRHPVVVAGTHGKTTTTALAAWLLAAAGRDPGYLVGGEPANFPGPAALGGGAPF
                     VVEGDEYSTSYADKGPKFLHYAPRTFVLTSVEFDHADLYADLAAVKAAFRAGVALVPE
                     DGAIVANGDDPNVREVLAAARTRVRLYGVAPRPAGAPDVDLLATQVAESEGGTRFTVH
                     RSGQEPYSVLSPLSGRHNVSNALAAFGAALGFGLHPAQLAGGLASFRGVKRRLEVVGT
                     EAGVTVVDDFAHHPTAVATTVAGAKNRFPGRRVWAAFEPRSMTAGRADFFDPYAAALG
                     AADAVALAAPFHAARLAQPGGPGVLDASALVARLRAAGKPALTAPTPEALLSALLAEL
                     RAGDVVVSMSSGAFGGFPRKLLDALRTR"
     gene            10249..11304
                     /locus_tag="PLB01_08075"
     CDS             10249..11304
                     /locus_tag="PLB01_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQQ77299.1"
                     /translation="MTPPRHSRLAGAIAAAALLALGLPSPLAAQAKAAKAAEAAGPKL
                     VIVEDKKDVGQVAKGEPIKHVFVLKNTGTADLHITDVKPSCGCTVPEFDKVIKPGAEG
                     RVTLTVDTKGFSGPISKTALLITDDPAVPQKTLFLSAVVKPFVDALPYAFFRITALSG
                     EAASAEVILVSDEADFKPTKAEAPNSFLRVALTPVPEKELVKDRGPNQWKVTVTTAPD
                     APEGLLGGAVRVMTGAKKQPEIELQVTGFIKPAVSVTPLSINFGNFDPKGDPQKRNVM
                     LVNNNAAVENFSVTKAETNVPGISAEVVPVDKSRVQVVLTVDQKIKKGVFEGDLTIKT
                     TDKSRAEVKVPIKGVIL"
     gene            complement(11377..12051)
                     /locus_tag="PLB01_08080"
     CDS             complement(11377..12051)
                     /locus_tag="PLB01_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77300.1"
                     /translation="MNRPLRVLGVLALAAVPAAAQQPDPLSTRLRTFTDTPIATFQGA
                     ECVSIDAPKSVVWRLLVGTETVSTWLLSGVPNVVPRRARYGKGLTASKGDVLSIDATT
                     ADGPRRIDLAVIALQPGEVLSFLLKSDAADLLDAKVESLTLSFFVDARPDGTTDLTWV
                     SHYDADSPFSALLSPTYMPARRARRLSALRMFKAIAEHAATLPYPPLHDVPTSPQPSS
                     TPRKRK"
     gene            12094..12501
                     /locus_tag="PLB01_08085"
     CDS             12094..12501
                     /locus_tag="PLB01_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77301.1"
                     /translation="MAFRALAALFLLATVPAAGDAPTPFRWEGLVRRNPGIVIFRSRW
                     NPGVLELKDDLLRWTDRTDPGKNLVLPVRRLTQHTLVCRGGPAAACTEWRVATKIETY
                     VFREAPPEGGASLRRAFDALRGAYADVPSSEER"
     gene            complement(12506..13027)
                     /locus_tag="PLB01_08090"
     CDS             complement(12506..13027)
                     /locus_tag="PLB01_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77302.1"
                     /translation="MKKPVLAAAALAALALTASCRRVDETRSLNFDPETTAGALGPGW
                     DGFEKTDSGDTFVWAHGKEARVVVQSRADGDRLVRFRCWPFGFPGGPGQTLTFSVNGR
                     KIQSVTLGGEPRVYTIPTSKDAWKRGANELTFAFTYAESPKDRIPGATDERTLAAGFD
                     WLEILPPASRPKK"
     gene            complement(13037..13393)
                     /locus_tag="PLB01_08095"
     CDS             complement(13037..13393)
                     /locus_tag="PLB01_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77303.1"
                     /translation="MRAAHAAVLAAATLALSAPARAADLYGKPLRGLTAVHVAAVVAA
                     PEKYAVKAVRVEGKNEGTAGRPVLKEGEAVLPIVSDGSWTLPEGLAGGTLAAEGKAQV
                     REGRAVFVAAGVEVRR"
     gene            complement(13390..14592)
                     /locus_tag="PLB01_08100"
     CDS             complement(13390..14592)
                     /locus_tag="PLB01_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77304.1"
                     /translation="MRLTLRFAVLALLIPAALRADTPATTQWVVTSAHASGGGNDYVT
                     GLRIVNPNAAAATVDLYLLPASDGSGDNSGAGKVAVTVPANATLAIDDVLSTKFGTVG
                     AGGIRVESTGSTKQPVWVLSQTLVVNARSSTGVPGTNGFAIPAQNTEQLVALGETAYV
                     PYVSSSSSGSSGYRTNLFLLSASGTGSTVVTVTLLKGDGTVLGTRDVTLAKYAQTQIN
                     GIAASFGYTADDTNLTATVKVKSGGPVATGASVIDNAIASISYSPPVKVAIANNGAFG
                     IALNDAYGAGGRLDIVDGNGDFVYTTMVVENCAGGPVGFSFQAFGPSFGPSLQNTSFV
                     RNPTDGTISFSGADPNSTFSGTILSRYDGSIQGTITYTRAAGSAGAPCGGATAAYAYQ
                     GAKVFKLP"
     gene            complement(14696..>14926)
                     /locus_tag="PLB01_08105"
     CDS             complement(14696..>14926)
                     /locus_tag="PLB01_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77305.1"
                     /translation="LGLQEHVAVGHLLVALAARAGASGASRLVVRLPAEAAALLEAAR
                     RMGLAAEEAIPFLALRRRGDFRRIAGAPGALF"
BASE COUNT         2191 a         5240 c         5473 g         2022 t
ORIGIN      
        1 cgcgtcgaac gcgaagaaga ggagaccgag gacgccgagc tcccgttcga cgtgattgag
       61 catccgcgtg tcggcgagcg ccgaggacac cgcttcgacc gacgcgagag gcaccatgag
      121 aaacgtgtta ccggtcccga cggcctgggg agggagtgcc gggtggagcg cgtcgaacgg
      181 gacgccgagc gccgccgcga tctggccctt cacgtcgtcg cccgtcacgg gcgccttcca
      241 ctcgggcttt ccctgctcca tccgcacgcg cgtgacgcgc ccgtccttct tcaggagctc
      301 gagcttgagc ggcccgaacc cgacctcgat cgtcaccggg tgaatgccgt ccggcgcgcc
      361 ggcggcggcc ctcccgagcg ccgccatcac gacggccgtc ccgaccgtcg gatggcccgc
      421 gtacgggatc tccttcaccg gcacgaacgt ccgctgccag taggcgtgcg cggcgtcctt
      481 cgcgggcagg acgaacgtgg actcggagta gttgatctcc cgcgcgatcg actgcatctg
      541 cgccgtcgtg aggccgcgcg cgtccggcac gacgaggagc gggttgcccg cgaaggggcg
      601 gtccgtgaac acgtcgcagg tgacgaagga gactctcatg gagacgacgt taccgcccgg
      661 aggcacgttc ggaaacgaca gaggccgacc atcgggagcg cccggtccag ggcgccgagg
      721 acggggtagg ggctcgcgga gtagaagagg tcctccacgc ggaagcccgc ctcttcgacg
      781 aggctcacca cgcgcgtggc ggggcgcacg gcgatgtggt cctcctgctg caggcccggc
      841 acggcggcct tgatccgctc ggcccagtgg tcccggttcg gcgtgtagat cgcgagggcg
      901 ccgccggaaa cgagcgaccg gcgcgcgctc tcgaggcacg ggacgagcac gtcgtcgagg
      961 acgtgctcga cgaagtcggc gaggagaacg acgtcgaacg ggcccccggg tgccgccgcg
     1021 agatcccgcg cgtcggccct ctcgaagcgg acgttcgaga ggccgcgggc ggcctgcccg
     1081 gcgcgggcgg cgtcgactgc gacgcctgcc gcgtcgatcc ccacgacctc cgcgacgcac
     1141 ggggcgagga agaacgccgt gtcgccgcgc gcggagccga cctcgaggac ccggagggac
     1201 ggagaggggc ggacgatccg gagcagcgcc gcgtcgcgct cctcgtactt tcgccggttc
     1261 cgcaggaacc agtgcgggcg gtacatccgg ccgtagtagg ccggcccgta gacgtcggcg
     1321 ttcggcgcga acttcccggg tgcatccgtc acgcgatgag tctacgcatc tgaagagaga
     1381 gccggcccct agaatggact cgatggcgcg cgtcacggtg atcgggggag gcttcgcggg
     1441 cgtggagtgc gcgcaccagc tcctccggcg cggccacgcc gtcacgctcg tcgagatgcg
     1501 acccgtgcgg acgacggacg cgcacgcgac ggaccgcctc gccgagatgg tctgttcgaa
     1561 ctcgttccgg tccgacaacc ccgcgaacgc cgtcggcctc ctgaagcgcg agatggagag
     1621 cgtcgggtcc ctgattctcg aggaggcgcg cagggccgcg gtcccggccg gcgacgcgct
     1681 cgccgtggac cggacgatct tcgcggaggc cgtcacgcgg cgcctcgccg gcgagccgct
     1741 cctcacgctc gtccggggag aggccgaggc gctgcccgac cccggcgacg ggttcgtcgt
     1801 cgtcgcaacc gggccgctca cctcgcccgc gctcgagaag gcgttgctgg gcctgctcgg
     1861 cgagaagtac ctctacttct acgatgccat ggcgcccatc gtcgaggcgg cgtcgctcga
     1921 catggcgaag ctctacgcgc tctcgcgcta cggcaaggga gggggcgacg actacctgaa
     1981 cgtcccgctc tcccgcgccg agtacgaggc cttcgtcgcc gccctcctcg cgggggaaaa
     2041 ggtcccgttc cacgacttcg agaaggccgt gtacttcgaa ggatgtcttc cggccgaggc
     2101 gatggccgag cgcggcgtcg agactctgcg ccacggcccg atgaagccgt tcgggctgcc
     2161 ggacccgcgc acgggcaggg agccgtatgc cgtggtccag ctccggcagg acgacctcgc
     2221 gaagacgcac ttcaacatgg tgggcttcca gacgaagctc aaggtcggcg agcagcggcg
     2281 cgtcttccgg atgctgccgg ggctcgagaa cgcggagttc gtccggtacg gcatgctcca
     2341 ccgcaacacg tacatcaacg gcccggcgca cctcgaccgt ttcttccgct ggcggaagga
     2401 cccgcgcgtc ttcttcgcgg gccagctcac cggcgtcgag ggctacctcg agtccgccgc
     2461 gacgggcctc atggtcggcg cggcgctcgc gcagctcctc gagggccggg agcccgcgcc
     2521 gctcgccttc tcgacggcgc tcggctcgct cgcccggcat gtctcgacgc cccgggaggg
     2581 ggacttctcg ccgatgaacg tgacgttcgg cctcatcgac gacgcggacg tcccgccgtc
     2641 gaaggatcgg gcgaaacgcc gcggggagat cgcgcgccgc gccctggaag gagtcgcgcg
     2701 ctggaagacg gaggctcttc ccgtgccgtc cgtcacggtc cccggcgcgg gcgccgccta
     2761 gaatcggcgc ggaggaagac tcgtgaagaa gaacctgacc gcagccctga ccgccgttct
     2821 cttcgccgcc gcgccggccg ccgtggccga cggccgctcc gagaacttcc agacgtactg
     2881 ctcggtctgc cacggcgacg acggaaaggg acagaccgag gaaggaaaga agaagggcgc
     2941 ccgcgacctc acgaacgcca agtggcagga caaggtcgac gacggccggc tgatcaagtc
     3001 cgtcacgaag gggcacgaca agatgcccgc cttcgagaag aagctctcgg ccgacgagat
     3061 caaggcgctc gtcgccgagg tgcggacgct cgcgaagaag tagtgcgcgg ccgcctgccc
     3121 ggggagctcg ccgcgttcga ccgccatctc gcggacgagc gcggcgtctc gcggcacacg
     3181 cgggcggctt acgggaagga cctcggtcgg tttgcggcgt ttctctcgac ggtcttctgg
     3241 aacaggccgc tcgagaagat cgcggccgcc gacgtcgacg cgctcgccgt gaggtcctac
     3301 ctcgcgcacc tgcgggcgga cggcctgacg aaggcctcga tcggccgcca cctctcggcg
     3361 ctccgcacgt tcttcgcgtt cctgaagcgc gagggtcacg tcgtggcgaa tcccgccaag
     3421 gcgatcgcga cgccccggaa ggagcggtcc ctgccccgca cgctctcggt cacggaggcg
     3481 ggcgccgtcg tcgaggcgaa agggcgggaa ggcgcgctcg gcgcgcgcga ccgggcgctc
     3541 ctcgaagtcc tctacgcgac gggtctgcgc gtctcggagg tcgtcgggct gaaactcgag
     3601 gacgtggacc tctccgcgcg gcaggtgcgg acggttggca aggggaggaa ggagcggatc
     3661 gtcccgttcg ggcgcgcggc ctgcgacgcc gtgaagacgt ggctccgcgc ccgggccgac
     3721 atgcgcccgg ccgcgaagga cgcggggttc ctgttcctga acgcacgcgc ggggcgcctg
     3781 acggaccgca gcgtgcggcg catcctcgac cgggcgatgc tgggggccga cgtgacccgt
     3841 cacgcctcgc cgcacgcgct ccggcactcc ttcgcgacgc acctcctcgc ggcgggcgcc
     3901 gacctgcggt cgatccagga gctcctcggc cacgcgtcgc tctcgacgac gcagcggtac
     3961 acccatctcg acgccgaacg cctcctcgag gtctaccgca agagccaccc gaaggcggag
     4021 gaccgggagt gagagagccc cggcgctaga ctccgcgcgt ggcccctccc gtcttccacc
     4081 acacgacgat cctctgcgtc aggcgcggat cctccgtcgc gctcggcggc gacgggcagg
     4141 tcacgatggg gaccaccgtc atgaagcacg gcgcctccaa ggtgcggcga ctcctcgacg
     4201 gcagggtcct cgcgggcttc gccgggtcca cggccgacgc cgtgacgctc ttcacgcgct
     4261 tcgagaagaa gctcgaggag ttccacggcc agctcgagcg cgcggccgtg gaactcgcga
     4321 cggactggcg gacggagaag acgctccgcc agctcgaggc cctgctcgtc gtcgcggacg
     4381 cgaaggtctc gctcctgctc tcggggacgg gcgacgtcat cgcccccgac gagggcgtcc
     4441 tcgcggtggg gtcgggcgga ccctatgccg ccgcggcggc gaaggccctg ctgaggcact
     4501 ccacgctcga ggccgcggcg atcgtcaggg agtctctcgc gatcgcggcc acgatcgaca
     4561 tctacacgaa cgacacgatc aaggtcgaga cgctgccgtg acgccgctga cgctgcgcga
     4621 cctgtcgccg cgcaagatcg tggccgaact ggatcgccac gtcgtcggcc aggcgaaggc
     4681 gaaacgatcc gtcgcgatcg cgctgcgcga ccgctggcgc cgccagcgcc tcccggccga
     4741 tctcgcgcgc gacacgacgc cgaagaacat cctcatgatc gggcccacgg gcgtcggcaa
     4801 gacggagatc gcgcggcggc tcgcccgcct cgtggacgcg ccgttcgtga aggtcgaggc
     4861 ctcgaagttc acggaggtcg ggtacgtcgg gcgggacgtc gactcgatcg cccgcgatct
     4921 cgtggacgcc gcgatccggc tcgtcaagga ggagcgccgg gcgcgcgtcg cggaccgcgc
     4981 ggcggcggcg gcggaggagc ggctcgtcga cctcctcgtg gccgcgctcg cgacggaccc
     5041 ctccggccgg gcggtcgcgc aggagggccg cgacaaggtg cgccgtgagc tccacgaggg
     5101 caggcacgag gcgaaggaga tcgagatcga ggtcgccgac gaccgaacgc cgcagatctc
     5161 cgtcatgggc ccgcagggca tggaggagat ggggatcgac atccccggca tgattcccgg
     5221 cctcttcggt ggcccgaaga agaagaaggt ccgcctgccg gtgtccgagg cgcggccgct
     5281 cctcctcgag cgcgaggcgg acgccctcac ggacgaggcg acgctccctt cggaagcgat
     5341 ccgccgcgcc gaggagacgg gcatcgtctt cctcgacgaa atcgacaaga tcgccgggcg
     5401 cgagggcgcg cggggcgggc ccgacgtgtc gcgcgagggc gtccagcgtg acctcctccc
     5461 gctcgtcgaa gggacgaccg tcaagacgaa gcacggcatg ttcaagacgg accacgtcct
     5521 cttcgtggcg gcgggcgcgt tccacgtcgc gaagccctcg gacctgatcc ccgagctgca
     5581 ggggcgcttc ccgatccgcg tctccctcga cgcgctcagc gaggaggact tcctccggat
     5641 cctgaccgag cccgagcacg cgctgccgaa gcagtccgcc gcgctcctcg gcgcggacgg
     5701 gatcacgctc gagttcgcgg acgacggcct cagggagatc gcccgcgctg cggcggaggt
     5761 gaaccgcaac ctcgagaaca tcggcgcgcg ccgcctccac acgatcctcg aaaccgtcct
     5821 cgaggagatc tctttcgcgg gccccgacgc ggcgggcgcc cgcgtcgtcg tcgacaaggc
     5881 gtacgtcgac cgcgcgctcg cgagcctcgt gcgcgaccgc gacctctcgc gcttcgtgct
     5941 gtgaggcgcg ccctcctcct cctcgcggtc gcgttcttcc ccggctgcgg gcgcaagctg
     6001 gcgcccgagg cgccgctgct cgtgctgccg gcccgcgtcg agcccgtgcg catctcgcag
     6061 gaggcgggcg acgtcgtgct gcgcttcccg tatccgtcgg tgacgaactc cgggtcgacg
     6121 ctcacgaacc tcacgaaggt caccgtcgcg cgcgagatcc agagcatccg cgaggggcag
     6181 cgcatcccgc cgccgccggc cgacgccgcc cagcgcgaga gcgaggagcg gaccttccgc
     6241 gcgcgcgccg agacggtcgc ggagctctcc gcacgcgacc tcgacgcggc cacggtcggc
     6301 gcggacctcg ttttccgcga cccgctcgtg ccgctctacc gcgagaagcg gctcgggcgc
     6361 gtcctgctgc ggtacgccgt cacggcgacc cgcgacaaga agcgcgtttc cgagctctcg
     6421 cccatcgcgt cgatcgtgcc ccgcgtgccg ccggaccggc cgctccgcgt gatcccggcg
     6481 gtcgaggaga accgcgtctg cctcgactgg tggccgcccc ccgcgatgct cgacggctcg
     6541 aaacccgtcg tcatcgccgg atacgcgatc taccggcgag acgcctccga ggaggagttc
     6601 gaggatcagc cgatcggcgt cgcgatcggc gccacgacgt acgtcgacgc gacgacccgg
     6661 ccggaccgcg cgtacgtcta cacggtccgc gccgccccgg tcgcggacgt cccgctcatc
     6721 ctcggcccgg ccgcggaccc cgttgccgtc tccacgaagg acgtctttcc gccgccggca
     6781 cccgagggac tcctcgtgct cgccgagtcg ggcggaacgc ggctcgtgtg gaacccgtcg
     6841 ctcgctccgg acctcgcgtt ctaccgcgtc taccggacgg acggcggggc gccggcgcgg
     6901 ctcgcggacg gactcaagga cccgctcttc ttcgacgcgg gggcctcggg acgaacctac
     6961 ggcgtgaccg ccgtcgacaa gtccggcaac gagagcgcgc cggggacggt gaaatgaaga
     7021 tctcgccgct cggcggcctt ccgatccgct tcacgaagat gagcggcgcg ggaaacgact
     7081 ttctcgtctg gggcgaggcc gtgagcgtgg gtgcggccga ggtcgccgcg atccagcgcc
     7141 tctgccggcg gggaaccggc gtcggggccg acggcgtcct cttcgtcttc ccggacgggc
     7201 ccggccgcgt ctcggcggac taccgcaacg ccgacggctc ggtgggccgc ttctgcggca
     7261 acggcacgcg ctgcgccgcg cgcttcgcgg tcctgaaggg gctcgcgccg gagaacctga
     7321 tcgtgaggac cggatggggc gacgttccct cccgcgtcga cggcacgcgc gtgacgctcc
     7381 ggctgcccga gcccgtcgcg gtgggccgcg cggtgtcctc gctcgacgcg tccggggcgc
     7441 tggagcgcga ggcgtacgcc ctcacggtcg gcgttccgaa cgtggtcgcc ttcgtcgccg
     7501 gggggatgga cgtcgcggcg ctcgatttcg cgcgcatcgg tccggccctg agataccacc
     7561 cggaactgcg cgagggcgcg aatgcggatg tcgcgcaggt gctcgggccg tcgcggctcc
     7621 gcgtgcggac ctgggagcgc ggggtcgagg ccgagacgct cgcgtgcggc tcgggcaacg
     7681 tcgcgacggc ggtcacggcc gcggcgctcg ggcgcgtcac gccgcccgtg tccctcgaaa
     7741 cgcgctccgg gcaggtcctg cgcgtggact tccgattcga gggcgaggtc gcgcgagacg
     7801 tcaccctcgc gggcgacgcc cgcgtcgtct acgaggggac gctggacccg ggggaatggg
     7861 aggacgcatg aggttcgtga ggctcgtgaa cggcggcgcg cccgcgtggg cgctggccca
     7921 ggaggagacg ctcgtcccgc tcgcgggagc gccgtgggag acgggcggcg tgccgaaacc
     7981 gtccggagac gcggtcccgc gccgcggggc gcgcctcctg cccgccgcct cgccgacgaa
     8041 gatcctctgc atcgggcgga actaccgcgc ccacgcggcg gagctcggcc acgaggttcc
     8101 gaaggagccg ctcgtcttct tcaagccgcc gtcgtccctc cttccgcacg gcgggacggt
     8161 ccgccttccg cgggagagcg cgcgcgtcga ctacgagggt gagctcgcgc tcgtgatcgg
     8221 ccggcgggcc cgccgcgttg cgaaggagga ctggcgggac gtcgtcttcg gcgtcacgtg
     8281 cgccctcgac gtgacggccc gcgacctcca gaagacggac ggccagtggt ggcgcgcgaa
     8341 gggcttcgac acgttctgtc cgctcggtcc cgccgtcgaa acggacgtcg acccgtcgac
     8401 gctcaccctc cagaccttcg tcgacggcgc gccccggcag aacgggaaca ccgcggactt
     8461 catcttcgac gtcgggacgc tgatcgcgtg gacgtcggcc gcgatgacgc tcgagccggg
     8521 ggacgtgatc ctcacgggca cccccgaggg cgtcgggccg ctcgcggccg gacagacggt
     8581 cgaggtgcgg atctccggcg tcggaactct ctcggtttcc gtggccgccg aggactagcg
     8641 ggtccggagc gcgtccagca gcttccgcgg gaacccaccg aaggcgccgg acgacatcga
     8701 aacgacgacg tcgcctgcgc ggagttccgc gagcagcgcc gagagaagag cctcaggcgt
     8761 cggcgccgtg agcgcgggct ttccggcggc gcggagccgc gccacgaggg cggaggcgtc
     8821 gagaacgccg ggcccgcccg gctgcgcgag ccgggcggcg tggaacggag ccgcgagggc
     8881 gacggcgtcc gccgcgccga gagccgccgc gtacggatcg aagaagtccg cccggcccgc
     8941 cgtcatcgag cgcggctcga acgcggccca gacgcggcgt cccgggaagc ggttcttcgc
     9001 gccggcgacg gtggtcgcga ccgcggtcgg atggtgggcg aagtcgtcga cgaccgtcac
     9061 gcccgcctcg gtcccgacca cctcgaggcg gcgcttgacg ccgcggaagg acgcgagtcc
     9121 gcccgcgagc tgcgccggat ggaggccgaa gccgagagcg gcgccgaagg cggcgagcgc
     9181 gttcgagacg ttgtgccggc cggagagcgg cgagaggacg gaatacggct cctggccgga
     9241 acggtgcacc gtgaagcggg tgccgccctc gctctcggcg acttgcgtcg cgaggaggtc
     9301 cacgtcgggc gcgccggcgg gcctgggcgc gacgccgtag aggcggacgc gggtccgcgc
     9361 cgccgcgagc acctcgcgca cgttcgggtc gtcgccgttc gcgacgatcg cgccgtcctc
     9421 ggggacgagc gcgacgccgg cgcgaaaagc ggccttcacg gcggcgaggt ccgcgtagag
     9481 atccgcgtgg tcgaattcga ccgacgtgag gacgaacgtc cgcggcgcgt agtggaggaa
     9541 cttcggtccc ttgtcggcgt aggaggtcga atactcgtcg ccctcgacca cgaacggcgc
     9601 gccgcccccg agcgccgcgg gacccgggaa gttcgcgggt tcgccgccca cgagatagcc
     9661 ggggtcgcgg cccgcagcgg cgaggagcca ggccgcgagg gcggtcgtgg tcgtcttgcc
     9721 gtgcgttccc gcgaccacga cggggtgccg gccgacgagg atctcgtcgt ggagcgtttc
     9781 gggcatcgaa cggacgttga gcccccggcg cagggcctcg aggatctcgg ggttgtcccg
     9841 gcgggcgagg ttgcccgcga cgacctcgga cacgtcggcg ccgacgttct ccgcgcggaa
     9901 ggcgggcagg acggtgatcc cgagagccgc gaggcgatcg gacatcggcg gatagacggg
     9961 gccgtcggag ccggtcacgc gttcgccccg ggcgaggagg agggaggcca gcggcgtcat
    10021 tgcggtcccc ccgacggcga ccaggtgaaa cgtacgaggg gctgcggaca ccgggcgcgg
    10081 agtttgctag acgggtcggg cgccgtcaaa cgacgcctcg ctggcgtgtc ccgccgggag
    10141 gccgagaaga ggcccggcgc accccacgta tactcgtcaa ggctcgaatc cagagtgttt
    10201 caagggtccc ggcgcggccg ggcccgctcc cgaaggaggc tcccggacat gactcccccc
    10261 cgccattccc gactcgccgg cgcgatcgcg gccgccgcgc tcctcgcgct cggcctgccg
    10321 tcgcccctcg ccgcccaggc gaaggccgcg aaggccgcgg aggccgccgg cccgaagctc
    10381 gtgatcgtcg aggacaagaa ggacgtcgga caggtcgcaa agggcgaacc gatcaagcac
    10441 gtgttcgtcc tcaagaacac gggcacggcc gacctgcaca tcacggacgt gaagccgtcc
    10501 tgcggctgca cggtgccgga gttcgacaag gtcatcaagc cgggcgccga ggggagggtc
    10561 accctcacgg tcgacacgaa gggcttctcg ggcccgatct ccaagacggc gctcctcatc
    10621 acggacgacc cggccgtgcc gcagaagacg ctgttcctct cggccgtcgt gaagccgttc
    10681 gtcgacgccc tgccgtacgc cttcttccgg atcacggctc tttccggcga ggccgcgtcc
    10741 gcggaggtca tcctcgtgtc ggacgaagcc gacttcaagc ccacgaaggc ggaagccccg
    10801 aactcgttcc tgcgcgtcgc gctcacgccc gtgcccgaga aggagctcgt gaaggaccgc
    10861 ggtccgaatc agtggaaagt cacggtcacg acggcgccgg acgcacccga aggcctcctc
    10921 ggcggcgccg tgagggtcat gacgggcgca aagaagcagc ccgagatcga gctccaggtg
    10981 accggcttca tcaagccggc cgtgtccgtg acgccgctct cgatcaactt cggcaacttc
    11041 gacccgaagg gcgatcccca gaagcgcaac gtcatgctcg tgaacaacaa cgcggccgtc
    11101 gagaacttct cggtcacgaa ggccgagacg aacgtcccgg gcatctcggc cgaggtcgtt
    11161 cccgtcgaca agtcgcgcgt ccaggtcgtc ctcacggtcg accagaagat caagaaaggc
    11221 gtcttcgaag gcgacctcac gatcaagacg accgacaagt cgcgcgccga ggtcaaggtc
    11281 ccgatcaagg gcgtcatcct ctgaggtaat cgggaaaccc gtcacgcggg tttcccgcaa
    11341 ccttccgctc ggccgctgcg cggcctgcgc ctgctcctac tttcgtttcc gcggcgtcga
    11401 ggacggctgc ggcgacgtcg gaacgtcgtg gagcggcgga tacgggagcg tcgccgcgtg
    11461 ctcggcgatc gccttgaaca tgcggagggc cgagaggcgg cgggcgcggc gggccggcat
    11521 gtacgtgggc gacaggagcg ccgagaacgg cgaatccgcg tcgtagtgcg aaacccacgt
    11581 caggtccgtc gtgccgtcgg gccgggcgtc gacgaagaag gacagcgtga ggctctcgac
    11641 cttcgcgtcg agaaggtccg cggcatcgga cttcagcagg aaggacagga cttccccggg
    11701 ctggagggcg atcacggcca ggtcgatccg ccgcggcccg tcggccgtcg tcgcgtcgat
    11761 cgaaaggacg tcgcccttgg acgccgtgag ccccttgccg tagcgcgcgc ggcgcggcac
    11821 cacgttggga acccccgaga ggagccacgt gctcacggtc tcggtgccga cgaggaggcg
    11881 ccagacgacg gacttcggcg cgtcgatgga aacgcactcg gctccctgga aggtcgcgat
    11941 cggggtgtcc gtgaaagtcc ggagccgcgt gctgagcggg tcgggctgct gcgccgccgc
    12001 ggggaccgcc gcgagggcga gaacgccgag gacccggagg gggcgattca tcgcgttcag
    12061 cataacggga aacgcggact ctacaattct ccgatggcct tccgggcgct cgccgctctt
    12121 ttcctcctcg cgaccgttcc ggccgccggg gacgcgccca cgccgttccg ctgggaggga
    12181 ctcgtccggc gtaacccggg aatcgtgatc ttcaggagcc ggtggaatcc cggcgtcctc
    12241 gagctgaagg acgacctcct gcgctggacg gaccgcacgg accccgggaa gaacctcgtc
    12301 ctgcccgtgc ggcgcctgac gcaacacacg ctcgtgtgcc gcggcggtcc ggcggcggcg
    12361 tgcacggagt ggcgcgtcgc gacgaagatc gagacgtacg tgttccggga ggcgccgccc
    12421 gagggcggcg cctcgctccg gcgcgcgttc gacgcgctgc gcggcgcgta cgcggacgtt
    12481 ccgtcgtccg aggagcgttg agaggctatt tcttcggccg cgaggcgggt gggaggatct
    12541 cgagccagtc gaagcccgcc gcgagcgtgc gctcgtcggt cgcgcccgga atccggtcct
    12601 tcggggactc ggcgtacgtg aacgcgaagg tcagctcgtt ggcgccgcgc ttccatgcgt
    12661 ccttcgacgt tggaatcgtg tagaccctcg gctcgccgcc cagggtcacg ctctggatct
    12721 tccggccgtt cacggaaaac gtgagggtct gcccggggcc gcccgggaag ccgaacggcc
    12781 agcaccggaa gcgcacgaga cggtcgccgt cggcgcggct ctggacgacg acgcgcgcct
    12841 ccttgccgtg ggcccagacg aacgtgtcgc cggagtccgt cttctcgaag ccgtcccagc
    12901 cggggccgag ggccccggcc gtcgtctcgg ggtcgaagtt cagggaacgc gtctcgtcga
    12961 cgcggcggca ggatgcggtc agggcgagcg cggcgagcgc cgcggcggcg aggacgggct
    13021 tcttcaccgg gcgagtctag cgccggactt cgacaccggc ggccacgaac acggcgcgcc
    13081 cctcgcgaac ctgggccttg ccctcggccg cgagcgttcc gcccgcgagg ccttcgggca
    13141 gcgtccacga gccgtcggag acgatcggaa ggacggcctc accctccttc aggacgggcc
    13201 ggccggcggt cccttcgttc ttcccctcga cgcgcacggc cttcacggcg tacttctccg
    13261 gagccgcgac gacggccgcg acgtgcaccg ccgtcaggcc gcgcagcggc ttgccgtaga
    13321 ggtccgccgc gcgtgcggga gcggacaggg cgagagtcgc ggcggcgagg accgcggcgt
    13381 gggcggctct cacgggagct tgaagacctt cgcgccctgg taggcgtacg ccgcggtcgc
    13441 cccgccgcac ggggcgccgg ccgagccggc ggcgcgcgtg tacgtgatcg tgccctggat
    13501 ggacccgtcg tatcgggaga ggatcgtgcc gctgaacgtg ctgttggggt ccgctcccga
    13561 gaacgagatc gtcccgtccg tggggttcct tacgaagctc gtgttctgga gcgagggccc
    13621 gaacgacggg ccgaacgcct ggaagctgaa cccgaccggc ccccccgcac agttttcgac
    13681 gaccatcgtc gtgtagacga agtcgccgtt gccgtcgacg atgtcgaggc ggccgccggc
    13741 gccgtaggcg tcgttgagcg cgatgccgaa ggcgccgttg ttcgcgatcg cgaccttcac
    13801 gggcggcgag tagctgatgg acgcgatcgc gttgtcgatc acggaagcgc ccgtcgcgac
    13861 ggggccgccg ctcttgacct tgacggtcgc ggtgaggttc gtgtcgtcgg cggtgtaacc
    13921 gaaagacgcc gcgatgccgt tgatctgcgt ctgggcgtat ttcgcgagcg tgacgtcgcg
    13981 cgttccgagg accgtgccgt cgcccttgag gagcgtgacg gtgacgacgg tcgagccggt
    14041 gccgctggcc gagaggagga agaggttcgt ccggtagccg ctggacccgc tgctggagga
    14101 cgagacgtac ggtacgtagg cggtctcgcc gagcgcgacg agctgctccg tgttctgggc
    14161 gggaatcgcg aagccgttcg tgcccgggac gcccgtcgag ctcctcgcgt tgacgacgag
    14221 cgtctgcgag aggacccaca cgggctgctt cgtcgagccg gtggactcga cgcggatgcc
    14281 gccggccccg accgtgccga acttcgtgga cagaacgtcg tcgatcgcga gcgtcgcgtt
    14341 cgccggcacc gtgacggcga cctttcccgc gccgctgttg tcgccggatc cgtcgctcgc
    14401 ggggaggagg tacaggtcga ccgtcgcggc ggccgcgttc gggttcacga tccggaggcc
    14461 cgtgacgtag tcgtttccgc cgccggaggc gtgcgcggac gtcaccaccc actgcgtcgt
    14521 ggccggcgtg tctgcccgga gggcggcggg gatcagaagg gcgaggacgg cgaaacgaag
    14581 ggtcaggcgc atgcggtcgg cttcctctcg gagagatttt gacccggatt ggacgcggag
    14641 cggccgccaa agttgcgacc ccgtctcccg gagtgtcgcc ggtgtctccg gtctttcaaa
    14701 acagggcgcc aggggcgccc gcgatccgcc ggaagtcgcc gcgccggcgg agcgcgagaa
    14761 aggggatcgc ctcctcggcc gcgagcccca tgcgccgcgc cgcttcgagc agcgcggccg
    14821 cctcggcggg gagccgcacg acgaggcggg acgctccgga agctcccgca cgggccgcga
    14881 gggcgacgag aaggtgacct acggcgacgt gttcctgcag gccgag
//
LOCUS       DAOMFY010000075        14880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1241_length_14880_cov_5.648229, whole genome shotgun sequence.
ACCESSION   DAOMFY010000075 DAOMFY010000000
VERSION     DAOMFY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14880
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1241_length_14880_cov_5.648229"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PLB01_08110"
     CDS             complement(<1..1631)
                     /locus_tag="PLB01_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HQQ77306.1"
                     /translation="MKPPTLRPALPILLLAGALFAPKQARAQRESYTYLSFVGPEVSL
                     VSPADEDSAARPNMPVLPGDVLVTGSGSRVEAVLSDGNVVRLDGRSELRFERLNRTYE
                     SDDDRTILVLARGSVAVEVREVAPDRALRVDTDDATIVSPARSFFRVDAGRRGTEVYV
                     LTGRVELNGRGGRVLVRAGEYAYVSADTDIELETAAVPRDRFTRFLEERYDRADRRDV
                     KRYVDSEYSYDYDAAGFEENGTWVYVPSAGKTCWRPNVPPDWTPYSLGTWRRTPAGLT
                     WVSYESWGWLPYHYGMWAWDPELGWYWIPGNLYAPAWVYWSYGDDWTGWCPVGWYGGY
                     YDSYYRSTRATFGNERGGPWIPNLRGKVEVTQIDRRGWNFAPTSRIGSRLDARDVTRG
                     DRVAFPRGQTVVVTTAPLRVERGTGPAASVPEAIRRASLSPESARGPVNEGFTSILRR
                     DRVLDAAAREELRRSLRTSREFGARAAPDVPRSRERADSWRAGTAPVRPGSDSAPARA
                     GSARSDEGWRSSTAVLPVPVERRGGESQRAGDSGWR"
     gene            1793..2308
                     /gene="def"
                     /locus_tag="PLB01_08115"
     CDS             1793..2308
                     /gene="def"
                     /locus_tag="PLB01_08115"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQQ77307.1"
                     /translation="MAIRDIVKYGDPRLVARNEIITDFEGPSLPQLIEDLKQTCWAAP
                     GLGLAAPQIGVNLRIAIVDLSVGKDPSQVLVLVNPVVVDTQGSIRDEEGCLSLPDFVE
                     VVERPEKVTIEALDERGVKRALDGRDLLARAFCHEIDHLDQRLFVDRLSSLKKGLVLR
                     KVLKRQKQGSW"
     gene            complement(2312..3214)
                     /locus_tag="PLB01_08120"
     CDS             complement(2312..3214)
                     /locus_tag="PLB01_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ77308.1"
                     /translation="MFRTAAAPPLILLVDDDPYQAEVASYIAYELGCDFESALSGTEA
                     LQKVDARRPDVVLLDVRMPDLSGYEVCRRIKTAAATAGTQVIFVTARTEEEDLLQGFE
                     ALANDYVTKPFSARELKARVKNALRVKQLVDTLTARAHLLELEQEIASRLDDEGISVA
                     ELRARLLVPALDRIAAVFGADGATLHVRRPGVAELHAAASTTWPGGAPPAYVAALALD
                     ADPRFGRAPRLAGAPEEEWTVAAAPLFAGSDLIGTLRVHRRGPAPAPGISHELEHLLD
                     FAGHFGRALRRVQVLDELRAKAGA"
     gene            3292..4797
                     /gene="cysS"
                     /locus_tag="PLB01_08125"
     CDS             3292..4797
                     /gene="cysS"
                     /locus_tag="PLB01_08125"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQQ77309.1"
                     /translation="MALRLHNTLTRSLEEFRPLVPGKVGLYTCGPTVYDRVHIGNLRT
                     FVWEDVMCRVLRALGYEVTQVMNLTDIDDKTIRGAVAAGVPLREFTERHVTTFFEDLK
                     ALGVVPAAVYPRATDHIPEMVEIVKKLAARGHTYESEGSVWFRIATFPDYGKLSKIDL
                     SAVMRGARVADDEYEKEDVRDFAVWKAAKEGEPESATWTTELGRGRPGWHLECSAMSM
                     KYLGETIDIHTGAVDNVFPHHENEIAQSEGATGKPFVKYWLHAEHLIVDGEKMAKSKG
                     NFFTLPALVEKGYTPRAIRYLFQSVPYRMKLNFTFDALKGAASALERLDSLDLRLSER
                     EKAGEKTEEKISLKVNAEAGGAFAGRVSAARAAVFAALEDDLNTAGALGALFSFVKAT
                     NVDLEAGNLSAADATAARGLLHDVAGNIFGVMNETAGRGVPYSPSKKSSSSSSESLSS
                     DEAWILERIEARKGAKKARDFKAADAIRDELLAKGIVLEDTPQGVRWKRKA"
     gene            4972..6537
                     /locus_tag="PLB01_08130"
     CDS             4972..6537
                     /locus_tag="PLB01_08130"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HQQ77310.1"
                     /translation="MDLTAEKTLLVASVAPASNAEAFAAAKDAASLPEGTAVEVRLDA
                     MSEAPDFLALRAAFGNRILIATVRSAQEGGSFRGTEDEEKNFLEGALAAGFDFVDVEF
                     RRDGAGRAWSSSLPPSKKISSLHDPEGIPADLDGVVARMLASGARYVKLVATANDASD
                     VLRLLEVQAADRSGRLAVFGMGEAGIATRVLSPYLGAALSFGALDPRRATAPGQIAAR
                     DLADVYAVGRPRRVRRLFALLGDRVSHSLSPALHNANFEVLGQDALYVPFALRSLGKE
                     LPPLVAGLDALGLPLVAASVTIPFKEEAAHLADADEKAVNTLVRVSGPGAPARFRPAN
                     TDRIAFEQVVPERSTKSGQGPALVLGAGGTARVAVDVLLRKGWEVLVANRTEERGEEL
                     ARATGALFLSKKSLATWPLPLVIVNATALGLRSGDPVPCPLEFLRPGVLVIDAPYRAE
                     GTELARAAQAAGATVVDGQTLLLLQAAGQATRFTGVETRGEDLLDRLAPRLRSCFTAA
                     WKARLAATAAGVA"
     gene            6534..8159
                     /gene="hisG"
                     /locus_tag="PLB01_08135"
     CDS             6534..8159
                     /gene="hisG"
                     /locus_tag="PLB01_08135"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQQ77311.1"
                     /translation="MKSDRPDPNLPRGVQALLFDAADRRRRAEDGVVAVLRAAGLREV
                     ILPVLDYADPYAGVTAEGDERLYRFLDREGQALALRADFTPMAARVIAPRLGQLDGTA
                     SLFYRGDVVRDEETGVGRPREFAQVGAERYGDARFEADEEMLMLLLSSLDVLPAERLR
                     LTLGHAGLLRKVIATAAPGLAEPDGVRLEYVMRGARERRVSSLSKLLLEGGASNTAAV
                     EIADGLLRGFDPSSGLFQAAATRAEAQRLRRVVDLVRRKQPALSVVVDLAATPDAPYY
                     TGLTFSVDAAGVPGVLAAGGRYDALLARFGVAAPALGFSIGLEALTAAAAAPESAAAR
                     PLRIAVGKGRLLGKTLAALGAGGASFGEPDGRRLLVPSSDGAFELLLLKDDDVPTYVA
                     HGGADLGVVGSDRVAESGEDVFSPIEFPFGECRLSLIGRAGEEFRPNGQPVRVGTKYR
                     RLAARYFDARKIAHEIVPLAGSVELAAALRLTDVVVDLIETGSTIAANGLAEIETIET
                     SRATVILGRASLVSRRSEIAAFLGRLREKAGAP"
     gene            8159..9490
                     /gene="hisD"
                     /locus_tag="PLB01_08140"
     CDS             8159..9490
                     /gene="hisD"
                     /locus_tag="PLB01_08140"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQQ77312.1"
                     /translation="MKIVDATTRAGQRELEALLASRRALPDFATLKQVLPIVERVLRK
                     GESALREYVERFDGPVAHGRGPRSSPSKKIFFSLDSSAKPTRAGGSEAEISSEFRRAF
                     RAARRRIEAYHRRQVPAGFAFTDRLGVSFVERPVPLDSVGVYVPGGRAFYPSSLLMGV
                     VPAQVAGVPRIVVATPPRAWALSPELRWAVRELGVDSVLLAGGAHGVAGLVSVAKVAK
                     IVGPGNRFVAAAKHLVSGLVGVDLPAGPSEVAILASADADPALVAADLLAQAEHDPDA
                     KCLLFTNSGRLAHAVAGEVVAQLKEFEEKISLKVMRAALRRGGRIFILKEISRAAAHA
                     SAFAAEHVQVMGRRAEKFAPDLMASAGALFIGSATPTALGDYVAGPNHVLPTGGAARA
                     YSGLSTRDFFRWGRSVSAPAAAGRRLARPAGVLARFEGLVAHAAALGRRVR"
     gene            9487..10545
                     /locus_tag="PLB01_08145"
     CDS             9487..10545
                     /locus_tag="PLB01_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ77313.1"
                     /translation="MTKAIPIRPEIRALQAYTPGVEEPFEIRAKLDFNESPDDVPEEI
                     RAEVLDRLEARRWGHYPEFGAPRLKKAIAASIGRSPDEIVVGNGSGETILAAVSVFAG
                     GGSLVLAPPTFSLYGQIAAIGSARVVAVPRTGDDFLVDEAAYLAAAERGVPLLCTPNN
                     PTGGVSGRPFVEKLLDTAPVVLLDQAYAEFAAAEDDFTGLVGARPNLVVFRTLSKAYA
                     AAGFRIGYAVAPPDLAREIDKAVLPFNVDLAAEELALALLARPEIAKARTERVIEERE
                     RVARSLRSMGHTVAPSSANFLFVAPRGSDAARVRRGLLERGVLVRDMTSAAAGRLRVT
                     IGLPSENDLFLSALQEVS"
     gene            10542..11138
                     /gene="hisB"
                     /locus_tag="PLB01_08150"
     CDS             10542..11138
                     /gene="hisB"
                     /locus_tag="PLB01_08150"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HQQ77314.1"
                     /translation="MSRTGRVDRKTKETSVSLSLDLDGTGRADVATPVGFLSHMLEAV
                     AKHAGFDLSVRADGDVHIDAHHTVEDVGLAFGEALDQALGTRAGLERFGASYVPLDEA
                     LARSVVDLSGRPYIVFESPVDKELLLVTKDFPFALVEEFWKSAAFRGKFNLHVDVIRA
                     RNGHHAAEAVFKSAARALRQACAVTGTEVLSTKGTLTA"
     gene            11135..11755
                     /gene="hisH"
                     /locus_tag="PLB01_08155"
     CDS             11135..11755
                     /gene="hisH"
                     /locus_tag="PLB01_08155"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQQ77315.1"
                     /translation="MNVPRVTLVDYGVGNVASVTRAFLRLGADVDFTSDLDRVAAASR
                     LVLPGVAAFAPAMQRLLETGLEAAVHAAVNRGGRLLGLCLGFQLLFEESLEHGRHKGL
                     GLVGGRVAPFPAGTRSPHVGWNQLRPTREDGLVKRLEDGSYVYFVHSYRPEGVAPADV
                     GATCDYGGGFPAIVQKANVWGCQFHPEKSSETGRLLLSNFLEEDAA"
     gene            11752..12528
                     /locus_tag="PLB01_08160"
     CDS             11752..12528
                     /locus_tag="PLB01_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HQQ77316.1"
                     /translation="MTAGRLALWPSIDLRGGRVVRLLKGEWDAVTTFDADPVEVARTF
                     EREGADGLHVVDLDAAFGMGTNKEEIQKILRVIRIPVQVGGGLRTGEAVEDFLEGVGT
                     ARAVVGSLPFLDRPAFINLLRRSSSFSSSSLPARIVVALDCKDGRPTVRGWTEDAGAG
                     DAVSVAREMAALGVGALLVTDVARDGAMLGPNLSLLSAVRAVFPGEILASGGMRGPED
                     LGPVDAALAGGPRGAIFGRALHAGATTVAALRSARDEVAA"
     gene            12525..13949
                     /gene="hisF"
                     /locus_tag="PLB01_08165"
     CDS             12525..13949
                     /gene="hisF"
                     /locus_tag="PLB01_08165"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQQ77317.1"
                     /translation="MTLAVRVIPCLDISGGRVVKGVSFEQLRDAGDPVEAAIRYEHEG
                     ADEIAFLDIAAPVEERGTLLGLVRRVANVLSIPFTVGGGVRSVEDADALLRAGADRVT
                     VNTAAVNDPSLLTRLSTQFGRQCVVLAIDGKRVRDLEGRMQMMVTTHGGRKLTERTVT
                     AWAAEAAAAGAGEILLTSVDADGTKEGFDLEMLRAVRGVVDVPVVASGGAGDLDDFAR
                     AVLIGGADAVLAASVFHDRVFSIVEVKAAMKRAGLAVRPVPPRATPLQPRYDANGLVP
                     VVVRDVETGDVLTLAWANAQALERTRETGLSHFYSRSRQALWKKGETSGHVQEVVAIS
                     FDCDRDAVLYDVKPVGPACHTGARTCFTRVDTLAPAEGDIGRLDLTALFATIADRRDR
                     PEADSYTNRLLESGPARIAQKVGEEAVETALAAVGGTDEALANEAADLLYHLAVLLTA
                     RGLAPKDVAAVLERRRGAPRRASK"
     gene            13946..>14880
                     /locus_tag="PLB01_08170"
     CDS             13946..>14880
                     /locus_tag="PLB01_08170"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HQQ77318.1"
                     /translation="MSAAVLRSVVVERPADLTTPVALMKALLAGDGPCFLLESVEGGD
                     RVARWSFLGAAPSGEISGASGDPFKLLKGLPAAAPGGGDDLPPFTGGAVGYAGYDAVR
                     RLEKLPATNPDPIGLPDAWFGIFDSVIAFDHARHRLLFVTHAGEGAEEERRAFERLRE
                     LEGRAFASSAPPRPAASPSLTFKETLPVSSYVAAVEKAKEHIRAGDVFQVVLSRRWSA
                     PFDGDPFAVYRALRSISPSPYQYFLRTPHGTIFGASPERLVRVAAAPKPRVADFNFGR
                     ETAEVFDDMLLRSVPMYAETQRMVGELVSDFAADKS"
BASE COUNT         2063 a         5247 c         5322 g         2248 t
ORIGIN      
        1 cgccagccgg aatcccccgc ccgctggctc tcgccgccgc ggcgctcgac ggggacgggc
       61 agcacggccg tcgaggagcg ccagccctcg tcgctccggg cgctcccggc gcgcgcgggt
      121 gcactatcgc tccccggccg gacgggggcg gttccggcac gccaggaatc cgcacgctcg
      181 cgcgagcggg gcacgtccgg tgcggcccgt gcgccgaact cccggctcgt ccggagcgag
      241 cggcggagct cctcccgggc cgcggcgtcg agaacacggt cgcggcggag gatggacgta
      301 aacccctcgt tcacggggcc gcgcgcggat tcgggggaga gcgacgcccg gcggatcgcc
      361 tcgggaaccg aggccgccgg acccgtgccg cgctcgacgc gcagcggtgc ggtcgtcacg
      421 acgacggttt gcccgcgggg gaaggccacg cgatcgccgc gcgtgacgtc gcgggcgtcg
      481 aggcgcgagc cgatgcgcga cgtcggcgcg aagttccagc cgcggcggtc gatctgggtg
      541 acctcgacct tgccgcgcag gttcgggatc cacgggccgc cgcgctcgtt tccaaaggtc
      601 gcccgcgtcg agcggtaata cgagtcgtag taaccgccgt accagccgac cgggcaccac
      661 cccgtccagt cgtctccata cgaccagtag acccacgcgg gggcatacag gtttccggga
      721 atccagtacc agcccagctc cgggtcccac gcccacatcc cgtagtggta cggcagccag
      781 ccccaggact cgtaggaaac ccacgtcagc ccggccggcg tccggcgcca ggtcccgagg
      841 gagtagggcg tccagtcggg cgggacgttc gggcgccagc aggtcttgcc ggcggacggg
      901 acgtacaccc acgtgccgtt ctcctcgaag ccggcggcgt cgtagtcgta gctgtactcg
      961 gagtccacgt aacgcttcac gtcgcggcgg tcggcgcggt cgtaccgttc ctccaggaat
     1021 cgcgtgaagc ggtcccgtgg aacggctgcg gtctcgagct cgatgtccgt gtcggcgctg
     1081 acgtacgcgt attcgcccgc gcgcacgagg acgcggcctc ctcggccgtt cagctccacc
     1141 cggccggtga ggacgtagac ctccgtcccg cggcggcccg cgtcgacccg gaagaacgaa
     1201 cgggccggcg agacgatcgt cgcgtcgtcg gtgtcgaccc ggagggcccg gtccggcgcg
     1261 acctcgcgca cctcgaccgc cacgctgccg cgggcgagca cgaggatcgt gcggtcgtcg
     1321 tccgactcgt acgtgcggtt gagccgctcg aaccggagct ccgagcggcc gtcgagccgc
     1381 acgacgtttc cgtccgaaag caccgcttcg acgcgcgagc cgctgccggt gacgaggacg
     1441 tccccaggca ggacgggcat gttggggcgc gccgcggagt cctcgtccgc cggggacacg
     1501 agggacacct cggggccgac gaaggacagg tacgtgtagc tctcccgctg cgcccgcgcc
     1561 tgcttcggcg cgaaaagcgc gccggcgagg aggaggatcg ggagggcagg tcggagcgtg
     1621 ggcggcttca tgacgcgtct ccgtcccctc ccatgcgacc cgcgtaccag cgccacgtcg
     1681 ccattctcgt cccccggcgg ggacgcttga ccggggccgg gggccaccgg cactctcggc
     1741 ggccgggacc gctccccaaa aggggattcc gggcttcggg gaggagatcc ggttggccat
     1801 ccgcgacatc gtcaagtacg gggacccgag gctcgtggcg cgaaacgaga tcatcacgga
     1861 cttcgagggc ccgtctctgc ctcagctgat cgaggacctc aagcagacct gctgggcggc
     1921 gcccggcctg ggcctggcgg cccctcagat cggcgtgaac ctccggatcg ccatcgtgga
     1981 tctgtccgtg ggcaaggacc cgtcccaggt gctcgtcctc gtcaatccgg tcgtcgtcga
     2041 cacgcagggc tcgatccggg acgaggaggg ctgcctgtcg cttccggact tcgtcgaggt
     2101 cgtcgaacgg cccgagaagg tcacgatcga ggccctcgac gagcggggcg tcaagcgggc
     2161 gctggacggc cgcgacctcc tggcgcgggc gttctgccac gagatcgacc atctcgacca
     2221 gcgccttttc gtggaccggc tctcgtccct gaagaagggg ctcgtcctgc ggaaggttct
     2281 gaagcgccag aagcagggct cctggtaggg gtcaggctcc ggctttcgcc cgcagctcgt
     2341 cgaggacctg aacgcgccgg agtgcgcgcc cgaagtgtcc cgcgaagtcg agcaggtgct
     2401 ccagctcgtg ggagatgccc ggcgccggcg cgggtccacg gcggtggacg cgcagcgtcc
     2461 cgatgaggtc gctgcccgcg aaaagcggcg ccgcagccac cgtccattcc tcctcgggcg
     2521 cgcccgcgag ccggggtgcg cgcccgaacc ggggatccgc atccagggcc agggccgcaa
     2581 cgtacgccgg gggtgcgccg ccgggccacg tcgtcgaggc ggccgcgtgc agctcggcga
     2641 ctcccgggcg ccgcacgtgc agggtcgcgc cgtccgctcc gaataccgcg gcgatccggt
     2701 cgagcgccgg aacgaggagg cgggcgcgca gctcggcgac cgagatgccc tcgtcgtcga
     2761 gccgcgaagc gatctcctgc tcgagctcga gcaggtgcgc gcgggccgtg agcgtgtcga
     2821 cgagctgctt gacgcgcagg gcgttcttca cgcgagcctt cagctcgcgc gccgagaagg
     2881 gcttcgtgac gtaatcgttc gcgagggcct cgaagccctg gaggaggtct tcctcctcgg
     2941 tccgggcggt cacgaagatg acctgggtcc ccgcggtggc ggccgccgtc ttgatccggc
     3001 ggcagacttc gtagccggag aggtccggca tccggacgtc gaggaggacg acgtcgggcc
     3061 gccgcgcgtc gaccttctgg agggcctcgg tgccggacag ggcgctctcg aagtcgcagc
     3121 cgagctcgta tgcgatgtag gaagcgacct cggcctggta ggggtcgtcg tcgacgagga
     3181 gaatcagcgg cggagccgca gctgtgcgga acatgcgcgt tcgggcctta tagcacaggg
     3241 ctcgagcccg ccggtggcgg cggaggcccg gatgcccgac aatcccgccg cgtggcgctt
     3301 cgccttcaca acaccctgac ccgctcgctg gaggaattcc ggcccctcgt gcctggaaag
     3361 gtgggcctct acacctgcgg cccgaccgtc tacgaccgcg tccacatcgg aaacctccgg
     3421 acgttcgtct gggaggacgt catgtgccgc gtcctcaggg ccctcgggta cgaggtcacg
     3481 caggtcatga acctcacgga catcgacgac aagacgatcc gtggcgccgt cgccgccgga
     3541 gtgccgctcc gggagttcac ggaacggcac gtcacaacgt tcttcgagga cctgaaggcg
     3601 ctcggggtcg tccccgcggc ggtctacccg cgcgcgacgg accacatccc cgagatggtc
     3661 gagatcgtga agaagctcgc cgcgcgcggc cacacgtacg agagcgaagg gtccgtctgg
     3721 ttccggattg cgacgttccc ggactacggg aagctctcga agatcgacct ttcggccgtc
     3781 atgcgcggcg cgcgcgtcgc ggacgacgaa tacgagaagg aggacgtccg cgacttcgcg
     3841 gtctggaagg ccgccaagga aggcgagccc gaatccgcga cgtggacgac cgagctcggg
     3901 cgcgggcgcc cgggctggca cctcgagtgc agcgcgatgt cgatgaagta tctcggcgag
     3961 acgatcgaca tccacacggg cgccgtcgac aacgtctttc cgcaccacga gaacgagatc
     4021 gcccagagcg agggcgcgac ggggaagccg ttcgtgaagt actggctgca cgccgagcac
     4081 ctcatcgtgg acggcgagaa gatggcgaag agcaagggca acttcttcac gctgcccgcg
     4141 ctcgtcgaga agggctacac gccccgggcg atccgctacc tcttccagtc cgtgccgtac
     4201 cgcatgaagc tgaacttcac gttcgacgcg ctgaagggcg cggcgtcggc gctggagcgg
     4261 ctcgattcgc tggacctgcg gctttcggag agagagaaag ccggagaaaa gacagaagag
     4321 aagatttctt tgaaggtgaa tgcagaggcg ggaggcgctt tcgccgggcg ggtgtcggct
     4381 gcgcgcgccg cggtgttcgc ggctctcgag gacgacctga acaccgccgg agcgctcggg
     4441 gcgttgttct cgttcgtcaa ggcgacgaac gtggatctcg aggcgggaaa tctctcggct
     4501 gccgacgcaa ccgccgcccg cggcctcctg cacgacgtcg cggggaacat cttcggcgtc
     4561 atgaacgaga cggccggccg cggcgtgccc tattcacctt caaagaaatc ttcctcttct
     4621 tcttcagagt ccctttcttc cgacgaagcg tggatcctgg agcgcatcga ggcccggaag
     4681 ggcgcgaaga aggcccggga cttcaaggcc gcagacgcga tccgggacga gctcctcgcg
     4741 aagggcatcg tcctcgagga cacccctcag ggagtccgct ggaagcggaa ggcttgacag
     4801 ggcggcccct gccggggtcc aatccgcgtt ccatgacgtc cgcttcgcgc cactggcaca
     4861 gccaccacac ataagccggc agcgcaacgc gccgccggcc tgatcgaggt ccggaggcgc
     4921 aggtcattca aggccgactc aagcccctcc ggaccggagg ggtttttcgt tttggacctg
     4981 accgccgaga agacgctcct cgtcgcctcc gtcgccccgg cgtccaacgc cgaggcgttc
     5041 gcggcggcga aggacgccgc gagcctcccg gaggggacgg cggtggaggt gcgcctcgac
     5101 gcgatgagcg aggcgccgga cttcctggcg ctgcgcgcgg cgttcgggaa tcgaatcctg
     5161 atcgcgacgg tccgctcggc gcaggagggc ggatccttta ggggaaccga ggacgaggag
     5221 aagaatttct tagaaggtgc cctcgccgcg ggtttcgatt tcgtcgacgt cgaatttcgc
     5281 cgcgacgggg cgggccgcgc gtggtcctct tctcttccac cttctaagaa gatctcttct
     5341 cttcatgatc ccgagggcat ccctgccgac ctcgacggcg tcgtcgcccg gatgctcgct
     5401 tccggcgccc gctacgtgaa gctcgtcgcg acggcgaacg acgcgtcgga cgtgctgcgg
     5461 ctcctcgagg tgcaggctgc ggatcggagc gggcgtctcg ccgtcttcgg gatgggggag
     5521 gccgggatcg cgacgcgcgt cctgtcgccg tatctcggcg cggcgctctc gttcggcgcg
     5581 ctcgacccgc gccgcgcgac ggcgccgggg cagatcgcgg cccgcgacct cgccgacgtg
     5641 tacgcggtcg ggcgcccccg ccgcgtgcgc cgcctcttcg cgcttctcgg cgaccgcgtg
     5701 tcgcactcgc tctcgccggc gctccacaac gcgaacttcg aggtcctcgg ccaggacgcg
     5761 ctctacgtgc cgttcgcgct gcgctccctc gggaaagagc tccctccgct cgtcgccggc
     5821 ctcgacgccc tgggcctgcc tctcgtcgcc gcgtccgtca cgattccgtt caaggaggag
     5881 gccgcacacc tcgcggacgc cgacgagaaa gccgtgaaca cgctcgtgcg cgtgtcgggt
     5941 ccgggcgccc cggcgcgctt ccgccctgcg aacacggaca ggatcgcgtt cgagcaggtg
     6001 gtcccggagc gatcgacgaa atcgggccaa gggcccgcgc tcgttctcgg tgcgggcggg
     6061 acggctcggg tcgcggtgga cgttcttctg agaaaggggt gggaggtcct cgtcgcgaat
     6121 cggaccgagg agcgcggcga agagctcgcg agagccacgg gcgccctctt cctttcaaag
     6181 aaatctctcg cgacgtggcc tctgccgctc gtcatcgtga acgcgacggc gctcggcctg
     6241 cgttcggggg atccggtccc ttgtcctctc gaattcctcc gtcccggcgt cctcgtgatc
     6301 gacgcgccct accgtgccga gggaaccgaa ctggcacgcg cggcgcaggc cgcgggcgcc
     6361 accgtcgtgg acggccagac gctgctgctg ctgcaggccg cgggtcaggc gacgcggttc
     6421 acgggcgtcg agacgcgtgg cgaggacctg ctcgaccggc tcgcaccccg gctgcgttcg
     6481 tgcttcacgg ccgcgtggaa agcccggctc gcggcgactg cggcgggtgt cgcgtgaagt
     6541 ccgaccggcc cgatccgaac ctgccgcgcg gcgtgcaagc gctgctcttc gacgcggccg
     6601 accggcgacg gcgggccgaa gacggcgtcg tcgccgtcct gcgcgccgcg gggctgcgcg
     6661 aggtcatcct gcccgtcctc gactacgcgg acccgtatgc cggcgtcacg gccgaaggcg
     6721 acgagcgtct ctaccggttt ctcgaccgcg aggggcaggc gctggcgctg agggccgact
     6781 tcacgccgat ggcggcgcgc gtgatcgctc cgcgcctggg ccagctcgac ggcacggcgt
     6841 cgctctttta caggggcgat gtcgtgcgcg acgaggagac cggcgtcggc cgcccgcgcg
     6901 agttcgcgca ggtgggcgcg gagcggtatg gcgacgcgcg gttcgaggcg gacgaggaga
     6961 tgctgatgct cctcctctcg tcgctggacg tccttccggc cgagaggttg cggctgacgc
     7021 tcggacacgc gggacttctt agaaaggtga tcgcgaccgc cgctccgggc ctcgccgagc
     7081 ccgacggggt acgcctcgag tacgtgatgc ggggcgcgcg cgagcgccgg gtgtcctccc
     7141 tttcgaagct cctcctcgaa ggaggcgcgt cgaacaccgc tgcagtcgag atcgccgacg
     7201 gacttctccg gggctttgac ccttcatcgg gtctcttcca ggccgccgcg acgcgggcgg
     7261 aggctcagcg gctcaggcga gtggtcgact tggtgcgcag gaagcagccc gcactctccg
     7321 tcgtcgtgga tcttgcggcg acgcccgacg caccgtacta cacgggactg acgttctcgg
     7381 tcgacgccgc gggcgttccc ggcgtcctgg cggccggcgg ccgctacgac gccctcctcg
     7441 cgcgcttcgg ggtggccgcg ccggcccttg gtttctcgat cggcctcgag gcgctcacgg
     7501 ccgcagccgc ggcccccgag tccgccgcgg cccgcccgct gcgcatcgcg gtcggaaaag
     7561 gacgtctcct cggcaagacg ctcgcggccc tgggcgccgg gggcgcgtcc ttcggcgaac
     7621 cggacggccg gcgcctcctc gttccgtcct cggacggggc gttcgagctc ctgctcctca
     7681 aggacgacga cgttccgacg tacgtcgccc acggcggcgc cgacctcggc gtcgtcggga
     7741 gcgaccgtgt ggccgagtcg ggcgaggacg tcttttctcc catcgagttc ccgttcgggg
     7801 agtgccgtct gtccctcatc gggcgggccg gcgaggagtt ccggccgaac ggccagcccg
     7861 tgcgcgtggg cacgaagtac cggcgcctcg cggcgcgcta cttcgacgcc cggaagatcg
     7921 cgcacgagat cgtgccgctc gcgggctccg tcgagctcgc ggcggccctc cgcctgacgg
     7981 acgtcgtcgt ggatctcatc gagacgggct cgacgatcgc ggccaacggc ctcgccgaga
     8041 tcgagacgat cgagacgagc cgcgcaaccg tcatcctcgg ccgcgcttcg ctcgtctcgc
     8101 gccgcagcga gatcgcggcc ttcctgggcc ggctgcgcga gaaggcgggt gcgccgtgat
     8161 gaagatcgtc gacgccacca cgcgcgccgg gcaaagagag ctggaggctc tcctcgcctc
     8221 gagaagggcc ctgcccgact tcgcgacgtt gaagcaggtc ctgcccatcg tcgaaagagt
     8281 tcttagaaag ggggaatccg cgctgaggga gtacgtcgag cgcttcgacg ggccggtggc
     8341 gcacggacgc ggtccccgtt cttcaccttc taagaagatc ttcttttctc tcgactcttc
     8401 cgcgaagccg acgcgtgcgg gcgggtccga agcagaaatc tcctccgagt tccgccgcgc
     8461 gttccgcgcg gcgcggcggc gcatcgaggc gtaccaccgc aggcaggttc ccgcgggatt
     8521 cgccttcacg gaccgcctcg gcgtctcgtt cgtcgagcgg cccgtgccgc tcgactccgt
     8581 cggcgtctac gtgccgggcg gccgcgcgtt ctacccgtcg tccctcctga tgggcgtcgt
     8641 gccggcccag gtcgcgggcg ttccgcgcat cgtcgtcgcg acgcctccgc gcgcctgggc
     8701 cctcagcccc gagctgcgct gggccgtgcg ggaactcggc gtcgactccg tcctcctcgc
     8761 cggcggagcg cacggggtcg ccgggctcgt ctccgtcgcg aaggtcgcga agatcgtcgg
     8821 cccgggcaac cgcttcgtcg ccgccgccaa gcacctcgtc tcggggctcg tcggcgtgga
     8881 ccttcccgcc gggccttccg aggtcgcaat cctcgcgtcg gccgacgcgg atcccgcgct
     8941 cgtcgcggcg gacctcctcg cgcaggccga gcacgacccg gacgcgaagt gcctcctctt
     9001 cacgaattcg gggcgtctcg cacacgcggt tgcgggggag gtcgtcgcgc agttgaagga
     9061 attcgaagag aagatttctt tgaaggtgat gcgggcggcc ctgcgccgcg gcggccgcat
     9121 cttcattctc aaagaaatct cccgcgccgc cgcgcatgct tcggcgtttg ccgccgagca
     9181 cgtccaggtc atggggaggc gcgcggagaa attcgcgccg gatctcatgg cctccgccgg
     9241 cgcgctcttc atcggttccg cgacgccgac ggcgctcggc gactacgtcg ccggaccgaa
     9301 tcacgtcctc ccgacgggcg gcgccgcgcg cgcgtattcg ggtctgtcga cgcgcgactt
     9361 cttcagatgg gggcggtcgg tctcggcgcc tgccgccgcc ggccggaggc ttgcacggcc
     9421 ggcaggcgtc cttgcgcgtt tcgagggcct cgtcgcccac gcggccgcgc tggggcggag
     9481 ggtgcgatga cgaaggcgat cccgatccgt cccgagatcc gcgcgctgca ggcgtacaca
     9541 ccgggcgtcg aggagccgtt cgagatccgc gcgaagctgg acttcaacga gtcgcccgac
     9601 gacgtgcccg aggagatccg cgcggaggtg ctcgaccggc tcgaggcccg ccgctggggc
     9661 cattacccgg agttcggggc gccgcggctg aagaaggcga tcgcggcgtc gatcggccgg
     9721 agcccggacg agatcgtggt cgggaacggg tcgggcgaga cgatcctcgc ggccgtctcg
     9781 gttttcgccg gcgggggttc cctggtcctg gcgcctccca cgttctcgct ctatggtcag
     9841 atcgcggcga tcggctccgc gcgggtcgtc gccgttccgc gcacgggcga cgactttctc
     9901 gtcgacgagg cggcctatct ggcggccgcg gagaggggcg tgccgctcct ctgcaccccg
     9961 aacaacccca ccggaggagt ctcgggccgg ccgttcgtgg aaaagctcct cgacacggcg
    10021 cccgtcgtcc tgctggacca ggcctacgcg gagttcgcgg cggccgaaga cgatttcacg
    10081 ggcctcgtcg gcgcgcgccc gaacctcgtc gttttccgca cgctctcgaa ggcgtatgcg
    10141 gccgcgggtt tccggatcgg gtacgccgtc gctccgccgg acctcgcccg ggagatcgac
    10201 aaggccgtcc tgccgttcaa cgtggacctc gcggccgagg aactcgcgct cgcgctcctc
    10261 gcgcggcccg agatcgcgaa ggcaagaacc gagagagtca tcgaggaaag ggagagggtc
    10321 gcgcggagct tgcggtcgat gggacacacc gttgccccct cgtccgcaaa cttccttttc
    10381 gtggcgccgc gcgggagtga cgccgcccgg gttcgccgcg ggcttctcga acggggagtc
    10441 ctcgtccgcg acatgacgag tgcggcggcc ggtcgcctgc gcgtcacgat cgggttgcct
    10501 tcggagaacg atctctttct cagcgccctg caggaggtct catgagccgt acgggacgcg
    10561 tggaccgcaa gacgaaagag acgtccgtgt ctctctccct cgacctcgat ggaaccggcc
    10621 gcgccgatgt cgcgacgccc gtcgggttcc tgtcgcacat gctcgaggcc gtggccaagc
    10681 atgcgggctt cgatctctcc gtcagggcgg acggggacgt gcacatcgac gcgcaccaca
    10741 ccgtcgagga cgtcggcctc gcgttcggcg aggcgctcga ccaggccctc gggacccgcg
    10801 cgggcctcga gcgtttcggg gcgtcgtacg tcccgctcga cgaggcgctc gcacgctccg
    10861 tcgtggacct ctcgggccgc ccgtacatcg tcttcgagtc gcccgtcgac aaggagctcc
    10921 tcctggtcac gaaggacttc ccgttcgcgc tcgtcgagga gttctggaaa tccgccgcgt
    10981 ttcggggcaa gttcaacctc cacgtggacg tgatccgggc gagaaacggg caccacgccg
    11041 ccgaggccgt cttcaagtcc gccgcgcggg cgctgcggca ggcgtgcgcc gtgacgggga
    11101 ccgaagtcct ctcgacgaag gggacgctga cggcatgaac gtcccgcgcg tcacgctcgt
    11161 cgactacgga gtcggaaacg tcgcgtccgt gacgcgcgcg ttcctgcggc tcggcgcgga
    11221 cgtggatttc acgtcggacc tcgaccgcgt tgccgcggcg tcccgcctcg tcctgcccgg
    11281 cgtcgccgcc ttcgcgcccg cgatgcagcg cctgctcgag acgggcctcg aggccgccgt
    11341 gcacgccgcc gtgaaccgcg gcgggcggct cctcgggctc tgcctcgggt tccagctgct
    11401 gttcgaggag agcctcgagc acggccgcca caaggggctc ggtctcgtgg gaggccgcgt
    11461 cgcgccgttc ccggccggaa cgcgcagccc ccacgtgggc tggaaccagc tgcgcccgac
    11521 gcgcgaggac ggcctcgtga agcggctcga ggacgggagc tacgtgtact tcgtgcactc
    11581 ctaccggccc gagggagtcg cgcccgcgga cgtcggggcg acctgcgact acggcggcgg
    11641 cttcccggcc atcgtccaga aggcgaacgt ctggggctgc cagttccatc cggagaagtc
    11701 ttcggagacg ggacggctcc tgctctcgaa cttcctcgag gaggacgcgg cgtgaccgcg
    11761 ggccgcctcg ccctctggcc ctcgatcgac ctcaggggcg gacgcgtcgt gcgccttctc
    11821 aagggggagt gggacgccgt gacgacgttc gacgcggatc cggtcgaggt cgcacggacg
    11881 ttcgagcggg agggcgcgga cgggcttcac gtcgtcgacc tcgacgcggc attcggaatg
    11941 gggacgaata aagaagaaat ccagaagatt cttcgagtaa ttcggattcc cgtgcaggtg
    12001 ggcggcgggc tgcgcacggg agaagcagtc gaggatttcc tagaaggggt gggaaccgca
    12061 cgcgcggtgg tcgggtcgct gccgttcctc gaccgccccg cgttcattaa ccttctaaga
    12121 agatcttcct ctttctcctc ttcttctctg ccggcccgca tcgtcgtggc cctcgactgc
    12181 aaggacgggc ggcccacggt gcgcgggtgg acggaggacg cgggggcggg cgacgccgtt
    12241 tcggtcgcac gcgagatggc cgccctgggc gtcggggcgc tcctcgtgac ggacgtggcg
    12301 cgcgacggcg cgatgctcgg gcccaacctc tcgctccttt ccgccgtgcg ggccgtcttc
    12361 cccggcgaga tcctcgcctc ggggggcatg cgcggccccg aggacctcgg gcccgtcgac
    12421 gcggcgctcg cgggcgggcc tcgcggcgcg atcttcgggc gcgcgctcca cgcgggggcg
    12481 acgacggtcg cggccctccg gtccgctcgc gacgaggtgg ccgcatgacg ctcgccgtga
    12541 gggtgatccc gtgtctcgac atctcggggg gccgcgtcgt caagggcgtc tcgttcgagc
    12601 agctgaggga cgccggcgac ccggtcgagg ccgcgatccg gtacgagcac gagggcgccg
    12661 acgagatcgc gttcctcgac atcgcggcgc ccgtcgagga gcgcggcacg ctcctcggcc
    12721 tcgtccgccg cgtcgcgaac gtcctctcga tcccgttcac agtgggcggc ggcgtgcgct
    12781 cggtcgagga cgccgacgcg ctccttcgag ccggcgccga ccgcgtcacg gtcaacacgg
    12841 cggccgtgaa cgacccctcg ctcctcacgc ggctgtccac gcagttcggc cggcagtgcg
    12901 tcgtcctcgc gatcgacggc aaacgcgtgc gcgacctcga aggacggatg cagatgatgg
    12961 tcacgacgca cggcgggcgg aagctcaccg agcggaccgt cacggcctgg gccgcggaag
    13021 cggcggccgc cggcgcgggg gagatcctcc tgacgtccgt cgacgcggac gggacgaagg
    13081 agggcttcga cctcgagatg ctccgcgcgg tccgcggcgt cgtcgacgtg ccggtcgtcg
    13141 cctcgggcgg cgcgggcgac ctcgacgact tcgcgcgcgc cgtcctcatc ggaggggccg
    13201 acgccgtgct cgcggcgtcc gttttccacg accgcgtctt ctcgatcgtc gaggtcaagg
    13261 ccgccatgaa gcgcgcgggc ctcgcggtgc ggcccgtccc cccgcgcgcg acgccgctgc
    13321 agccgcggta cgacgccaac ggcctcgttc cggtcgtggt ccgcgacgtc gagacgggcg
    13381 acgtcctcac gctcgcctgg gcgaacgcgc aggcgctcga acggacgcgc gagaccggcc
    13441 tctcccactt ctacagccgc tcgcggcagg cgctctggaa gaagggcgag acctcggggc
    13501 acgtgcagga ggtcgttgcg atctcgttcg actgcgaccg ggacgccgtg ctgtacgacg
    13561 tgaagccggt cgggcccgcc tgccacacgg gcgcgcggac gtgcttcacg cgcgtggaca
    13621 cgctcgcgcc cgccgagggc gacatcggga ggctcgacct cacggcgctc ttcgcgacca
    13681 tcgccgaccg gcgggaccgc ccggaagccg actcgtacac gaaccgcctg ctcgagtccg
    13741 ggcccgcacg catcgcacag aaggtcggcg aggaagccgt cgagacggcg ctcgccgccg
    13801 tgggcggcac ggacgaggcg ctggccaacg aggccgcgga cctgctctac catctcgccg
    13861 ttctcctgac ggcccggggc ctcgcgccga aggacgtcgc cgcagttctc gagcgccggc
    13921 gcggtgctcc gcgccgggct tcgaaatgag cgcggccgtc ctccgctcgg tcgtcgtgga
    13981 gcggccggcg gacctcacga cgcccgtggc cctcatgaag gcccttctcg cgggcgacgg
    14041 gccgtgcttc ctcctcgagt cggtcgaggg cggcgaccgc gtcgcgcgct ggtctttcct
    14101 cggcgccgcg ccgtccggcg agatctcggg cgcatcgggc gaccctttca agctcctcaa
    14161 gggcctgccg gcggcggcgc cggggggcgg cgacgacctg ccgccgttca cgggcggggc
    14221 cgtgggctac gcgggctacg acgccgtgcg gcggctcgag aagctccccg cgacgaaccc
    14281 ggacccgatc ggcctccccg acgcgtggtt cgggatcttc gactccgtca tcgcgttcga
    14341 ccacgcccgc caccgcctgc tcttcgtgac gcacgcggga gagggagccg aggaagagag
    14401 gagagccttt gaaaggttgc gagagctcga aggcagggcc ttcgcaagca gcgcgccgcc
    14461 gcggcccgcc gcctcgccct ctctcacctt caaagaaacg cttccggtct cttcgtacgt
    14521 tgccgcggtc gagaaggcga aggagcacat ccgcgccggc gacgtcttcc aggtcgtgct
    14581 ctcacgccgc tggtcggcgc cgtttgacgg cgacccgttc gccgtgtacc gggccctgcg
    14641 ctcgatcagc ccgtcgccgt accagtactt cctgaggacg ccgcacggaa cgatcttcgg
    14701 cgcctcgccg gagcggctcg tgcgcgtcgc cgccgcgccc aagccgcgcg tggccgactt
    14761 caacttcggg cgcgagaccg ccgaggtctt cgacgacatg ctcctgcgct cggtgccgat
    14821 gtacgccgag acccagcgca tggtcggcga gctggtgagc gacttcgcgg ccgacaagtc
//
LOCUS       DAOMFY010000076        14874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1243_length_14874_cov_7.481004, whole genome shotgun sequence.
ACCESSION   DAOMFY010000076 DAOMFY010000000
VERSION     DAOMFY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14874
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1243_length_14874_cov_7.481004"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLB01_08175"
     CDS             <1..486
                     /locus_tag="PLB01_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQQ77319.1"
                     /translation="GSPCPVCRGLGEVVANEKITIRIPAGVSTGSKVRVAGKGRTEEG
                     DLYVALTVEPHPYFGREGDDILTEVPVTVPEAYAGAEIEIPTIHGPVRAKIPPGTAAG
                     QRFRLKGFGVRSGRGADGDHYYRVTIATPERVTDEGRKIAEKFAALYERNPRANLPSG
                     L"
     gene            complement(619..1737)
                     /locus_tag="PLB01_08180"
     CDS             complement(619..1737)
                     /locus_tag="PLB01_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77320.1"
                     /translation="MTTAFERPFAARLRAALLGLAACVLVAGARADAANAVLAKDGTL
                     YEVFVTNYGQIVEGANGTDSSMPVVALRSTPPGKPSTVEIVGGTVTPLLKFSESLEYD
                     DTTQTVFVVYTSLQVQGFFADVRAAMRRDGGWSNGGFLPNAGLYFSVNPKLLVTRQRY
                     KDLDDEGAAVTKTRSILSVVWWEETALSQARYAALFIEDGSMKFDEVAAYNLNELAGA
                     AGPTDSRGLPTSSYMYPAVQRDQVGDGGVLVSFANLATRKQQVLRLGFPDDYTKPPTT
                     GTTTAGRTSNSRTTPIGRGGYDFAMPTMIDLPFTLPVGTLISPAGVPTYFWTKDGGLN
                     YLTGDAASLARGVMTIPLRQDFQLDSALSVVRTMAEKQ"
     gene            complement(1742..2656)
                     /locus_tag="PLB01_08185"
     CDS             complement(1742..2656)
                     /locus_tag="PLB01_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77321.1"
                     /translation="MAEPAVDARSAAEASRREALSHLEKGEFAAALHCYDAALASARA
                     TDEPAFVDWIYVCRAAAAAETGPASDELVELKRILLRNREPQTAFRAAYSSAAIYRLR
                     KERNKVFFYANLARRHAEEIGDPRLIGACLNEVGNALAADSRFEEAAEAYLSALERTR
                     ADAPLLSVPRAQWSDNLGYCRIALDRVAEGLALVHDALDTLEKEAARGFTVYPLLDLC
                     FGYLKQDRFEEARWFGEEGLARVAVAGDLEAEKNLLYLLGEACHLSGDAPAAQGYFDR
                     LARLYPDFKNLRAYLDVFDFRNVINLRS"
     gene            complement(2649..3047)
                     /locus_tag="PLB01_08190"
     CDS             complement(2649..3047)
                     /locus_tag="PLB01_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ77322.1"
                     /translation="MSAVPRASQILLVGQKIRQIRKSRHLTQADLAARIGVTQSDLSR
                     MENGEYKVGLDTLFRILQVFELSMSHFFEEPSAEPAAAAKTPGAAAPFEDWAALPEDS
                     RREVAEFIAFKKMQIEKAKAGEDPGDDTNG"
     gene            3126..3893
                     /locus_tag="PLB01_08195"
     CDS             3126..3893
                     /locus_tag="PLB01_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ77323.1"
                     /translation="MKNKVFLLSQLLRRDLAARYAGSFAGPFWAILNPAILCGLNAFV
                     FAYVIKLRPPAGFGGTFVEFLLAGMLPWLGIQEAIVRGTTSVTDQAHLVKKLKFPVAL
                     LTLSSVGAALVIQTISIALLTCYLLVAGRGSVDPLLLAAAFGFELLLLVGPALILASL
                     NPFFRDLAQILPPVLMIAFYVTPILYPESMMPDWAAPLLTLNPLRDVIALFRAALFGM
                     PAPPLRRLGIEAAAFIVLGFLGRQLYRRLRPAFADVL"
     gene            3958..5652
                     /locus_tag="PLB01_08200"
     CDS             3958..5652
                     /locus_tag="PLB01_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQQ77324.1"
                     /translation="MKPTFAVIAVAAFSLLAGCSSSTAPKTASAPAPAPDAKKGAPGP
                     DEVMGLRVPGPVEGEDERTLPPKPYFDPEFGKRVVRIPKGDPMSQYYEKEGRLFLAIV
                     AGPGVPILRSDADAWIVEAPKERKIAEKSATGAPEAPVIIEMPKSESEAVTPKTSKET
                     LRFEEISGGLPRSGMWRENFDLGDVLGTGRPQIVAPPARLTGKSVQVFRLDRDAAGAP
                     RWRVVELEMENPENVDAAYGAATVGDIDGDGKLDIVFGGHGSGPAIAFNKGDGKFRIE
                     TRGLPRQMSTRAIAVGDLNGDGKLDLLAISDEMEWSKAGGKPSFDKDSTYERGYDVRA
                     FLNEGKTFKDVHKGTEGACFGYSIALAVPKTPEAGLPFYASACRYVGINAMLYEFDAK
                     TEKFRDLWFGVAEAHTQHTGAAAGTYRGRPAVFGSWFKRSPFGATPKIDGEGVTAYYR
                     GTDGKMETKRVVKTLKFVAASYAVAAGDLNGDGLDDVVWADEAAGRIRVFFQTAEGEF
                     EELAADREPTFINHPTCLRIADVDGDGRADVVLMYQYLTGDETRAGGFRVFRGLGK"
     gene            5940..7592
                     /locus_tag="PLB01_08205"
     CDS             5940..7592
                     /locus_tag="PLB01_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77325.1"
                     /translation="MKKFSVLIALALLAVAGTGYAVTCSYDNVPGATLLVPYWKVSLN
                     GATDAPIPTGGIDTLVSIVNVSNPGVIAHVTVWNKYSKAVIDFNLPLTGKDVASFSMR
                     DIMNGALSPNLPQYNTTKYPIDPCGLKNPGLVNVTYVPSIGWGQTQFIRFSHPEATAA
                     GLTTDVLRSISQYNLTSTDAIGSVFRPVVWDSLDESGDISTHISSAGANILDKDNPQC
                     GWGSSDGTYSGALSGYLTIDVVNYCTNYFPDQAQFYNEDAIATSGWSQSGYTPNVLIG
                     DVFYIDSATNGGNISGDPAIPVEFDARLNWTAGGGTKANPVKTFFGKYVWDKAEPACP
                     AEDGGGCVTVNVPAPFIFGGDGREPLGDRYGFRYLANASAGYQTWSLVWRSDLYATAT
                     TLTSNLCTWANPNSGANKGASGTGLYDALHQVTYVTFDNDENVYGGVGIPPGPSGNNP
                     SQNTIQTYLFLESQRIDLLKATAKDADWNPAQFTGGWADLTLRGATMTGLTAATGFYN
                     QGWVGVQHTAPGAVLSVGHAAASLSNQFQCSPALVFGPGNTP"
     gene            7830..12923
                     /locus_tag="PLB01_08210"
     CDS             7830..12923
                     /locus_tag="PLB01_08210"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQQ77326.1"
                     /translation="MKPLRSTRNARLVTGAFGLVLSAFLLAGAPAALGQGGCIVSTPT
                     TFEPNPPGPAAPRNVGLLSPVAFNHLSLYQGSSGAARILMQETFGYSTLDLTNPAQPS
                     VLRYDYFPFDTNKVPINGDGQSYTQAITASSDGQRVALSLNGPIPAWANTVVGYPDGN
                     NGWTLWGDTAPVGGVGNAILKSGSRYVHYGMTSFQFYASDVTNQPATFAGSNIPAELT
                     GLPGGARLSTAGSYLVYTTATGDIQIIDASNPGPAGSIASGLKWTTIPSSSPMFNGHS
                     IGATKSALEPGNAKMLWILAELQPASGENSPSYALFSVTQDLAGNFGTPVPAGAPFRV
                     PSVVGDNWTSAGNSSYLAEHNGTLYVLMWAFRRTPSIQYVLHSTTTSAWTTGSFGVPQ
                     TITDPTFSLGEMKTLASGANLYGYMASGTSAWVVPMTCTAPNAKATAILTAVNPVTGV
                     TVNSGDAVVYGETLTFAAQVQPTPTQGQNSDLAGFRWNFDFDFHTGSLVEDNGVGTSP
                     RVKNNDNGEFGNSAYPPSGTFNLVGPCDPKNGGVRGTGTGCWASVTTNTAQGGPDFTG
                     SEAAGAVKPLVVAFEANNANGSNGANLFTINWKRPTIGLVSSAIIAGDSLVASYDGHP
                     QSQDPTFPWKWYLDGALVNCNTGSACIPAGANATAGQHTYWVTVPYAQIGYSTPDYTG
                     TPMGTYTVSTFVPIFSVNGSATGPVGTTPSGTLTVANASKRANTNITGAYSYCIVPAG
                     QSCSSYTSWSMADPTNTNGSPASSATIPNPGVGNWILKIRVAYTGGTVDWPDANPANG
                     ISLLITNSLSVSASVSPNPANHNDTVVFHCSATGGSGNYNYKWSGQFGDVAFTQDYTT
                     RVTNTGGQDSSFSLDCSATDATSGASGFATAAVTVHPGAPAGAPTASASVSPNPVEVG
                     RPATFSCTGTGGTGALTYKWTDSLFHEFSGQTYTYIPTSQAPVTLTCTVTDSANRTGT
                     SSATLFVVPPPGPACPAVDWGVYVDGQELLPTPGQFGLEFPRTASVGTPLFFSIKSGI
                     SSQFEWNWGDGQVTTLTLAQYQANPPTHAYTTTGLKTITLRPQFVDDSGFCGGRSYDI
                     QITGPNANFTPGYLDGSPFVVADVTAGKAVRFSASEGGATSYDWDFGDGLPHGSGQVV
                     THIFTTAGARTVTLTVVKGVTVSSSLTLTVHLPPEPPKWVVPGMAYLAGSGGAFWTSD
                     VTIFNPDKVLAQKLSVAFLDPRNLVTDYSQLTFVPLADIPPLGALAGSNALQALGKPE
                     GSYGALLFKGTLGQALAPVVVTRTFNDQSSQGKGTFGLSVPSASAATGGVTAQAAPAV
                     QMLVGLRQDASARTNLAIVNLNNEWPTVKLDLYDGRTNSILGSKTLPLNPYQVLQVDR
                     VLETVAGAGSTSDLFKVQVSVVDQSGGAHAYAVYPYATVIDAYSTDPIVVTPTSTPAA
                     AYRIPGIVRLTGANGEKFRSRVTVSNPSLASRIVRMTYSFVSCDASGACGDRKTYSST
                     VGMAPGQTQAWDDWVKVWFQAKYEAILDEAKNYKDSLLDVTADDGNTDPLVVLGETYS
                     DLGTAPDGTSRGHVGLQVPGYPAADGASRAGSGANGNTRLVLTGLRSDAAYRTNLALF
                     VTSGTAGGWCRVRVYAPEGTLLKDVPVEVRPVMQVSGASLVAGIPGSLPRYSLVIDNF
                     DEGIVVGGYATIIDNASADSTFVKALPVP"
     gene            13006..14190
                     /locus_tag="PLB01_08215"
     CDS             13006..14190
                     /locus_tag="PLB01_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ77327.1"
                     /translation="MSDVSTTYSFTDLSKVYRLYSAPRDRLVEALTGRPRHAEVHALR
                     GVSGSIDRGAVLGVIGENGAGKSTLFKILAGTTAATSGSVNLPGRIASILELGSSFHP
                     DDTGRSNVILQAALAGLSREEVSAALPEIEEFAELGDFFDRPVKTYSSGMQMRLAFSV
                     ATAVLPDVILLDEALAVGDGRFQKKCVDRIFELKASGKTILFCSHAMYYVSTLCDRAI
                     WLNAGKVAAEGDAQSVVLEYERYLARKDSVSAAHETPPNPSTHGRILGLSFRDSAGNP
                     AAEFRPGEPWIAEIDVEADSPERPLQVHLGISTRDSVVCFSADSRLDGFGPFRGARQY
                     RLRIVVDALPIGKGEFVATLFVGDENALALYDSRSAVFHVESSTWRSGLLAVRPRWEF
                     RS"
     gene            14187..>14874
                     /locus_tag="PLB01_08220"
     CDS             14187..>14874
                     /locus_tag="PLB01_08220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ77328.1"
                     /translation="MIAVLAGPAPDGRADRAAFTRSHDAWRGESGPDLLVVEPDDPRA
                     PSAGLRLDVAEAEASVAPGGLARAAAILLASDASSVEPAGFWRGSPRTPEPYATVWEF
                     EEVAARTPGPPSSLPSDSPAAPVALRRAEGGDGSRLSTEAWTVHSFAFRRGHARPEVA
                     ALVPPGSARILEIGCGEGALGAVLERAGARVTGVEPDAGAARVAASRLSRVVPRGLDE
                     AWDEIGEGWAA"
BASE COUNT         2326 a         5209 c         4994 g         2345 t
ORIGIN      
        1 gggagcccgt gcccggtgtg ccgcggcctc ggcgaggtcg tcgcgaacga gaagatcacg
       61 atccgcatcc ccgcgggcgt ctccacgggg tcgaaggtgc gcgtggcggg gaagggccgc
      121 acggaagagg gagacctcta cgtggcgctc acggtcgagc cgcaccccta tttcgggcgc
      181 gaaggcgacg acatcctcac cgaagtcccc gtgacggtgc cggaggcgta cgcgggcgcc
      241 gagatcgaga ttccgacgat ccacggcccc gtgcgcgcga agatcccgcc ggggaccgcg
      301 gccggacagc gcttccgcct caagggattc ggggtgcgca gcggtcgcgg cgccgacggc
      361 gaccactact accgcgtcac gatcgcgacg cccgagcgcg tcacggacga gggacggaag
      421 atcgccgaaa agttcgccgc gctctacgag cgcaacccgc gcgcgaacct gccatcaggg
      481 ctctaggaaa agaccaagag ggattttctt ggaaggtgaa cagggcgatt cgtgcggctt
      541 gctcaactct ggcaagggag tgagcacccc tcttcaaatt ctctgcagaa gaaacgcccg
      601 gaaaacgaaa agcggcgtct actgcttctc ggccatcgtc cggacgacgg agagcgcgct
      661 gtcgagctgg aagtcctgcc ggagcgggat ggtcatcacg ccgcgcgcga gcgacgcggc
      721 gtcgccggtg aggtagttca gcccgccgtc cttcgtccag aagtacgtcg ggaccccggc
      781 gggcgaaatg agcgtcccga ccggcagcgt gaacggcagg tcgatcatcg tcggcatggc
      841 gaagtcgtag ccgccgcgcc cgatcggcgt cgtccgcgag ttggacgtcc ggcccgccgt
      901 cgtcgtgccc gtcgtcggcg gcttcgtgta gtcgtcgggg aagccgaggc gcaggacctg
      961 ctgcttgcgc gtcgcgaggt tcgcgaacga gacgaggacg ccgccatcgc cgacctggtc
     1021 gcgctgcacg gcggggtaca tgtaggagga cgtcgggagg ccgcggctgt cggtcggccc
     1081 ggcggcgccc gcgagctcgt tgaggttgta cgcggcgacc tcgtcgaact tcatcgagcc
     1141 gtcctcgatg aagagggccg cgtagcgggc ctgcgagagc gcggtctctt cccaccacac
     1201 gaccgagagg atcgagcgcg tcttcgtgac ggccgcgccc tcgtcgtcga gatccttgta
     1261 gcgctggcgc gtcacgagga gcttcgggtt caccgagaaa tagaggccgg cgttcgggag
     1321 gaagccgccg ttcgaccagc cgccgtcgcg gcgcatcgcg gcgcgcacgt cggcgaagaa
     1381 gccctggacc tgaaggctcg tgtagacgac gaagaccgtc tgcgtcgtgt cgtcatattc
     1441 gagagactcg ctgaacttca ggaggggcgt cacggtgccg cccacgatct cgacggtgga
     1501 cggcttcccg ggcggcgtcg agcggagagc gacgaccggc atcgaggagt cggtcccgtt
     1561 cgcgccttcg acgatctgac cgtagttcgt gacgaagacc tcgtagagcg tgccgtcttt
     1621 cgcgagcacc gcgttcgcgg cgtccgcccg tgcgcccgcg acgaggacgc acgccgcgag
     1681 cccgaggagg gcggcgcgca ggcgggcggc gaacgggcgc tcgaaggcgg tggtcacggc
     1741 gtcagctccg gaggttgatg acgttgcgga agtcgaagac gtcgaggtag gcgcgaaggt
     1801 tcttgaagtc cggatagagt cgcgcgaggc ggtcgaagta gccctgggcc gcgggcgcgt
     1861 cgcccgagag atggcaggct tctccgagga ggtacaggag gttcttctcg gcctcgaggt
     1921 cgccggcgac ggcgacgcgc gcgaggccct cctcgccgaa ccagcgggcc tcctcgaagc
     1981 ggtcctgctt gaggtagccg aagcagaggt cgaggagcgg atagaccgtg aagccgcggg
     2041 cggcctcttt ctcgagcgtg tcgagcgcgt cgtgcacgag cgcgagcccc tcggccacgc
     2101 ggtcgagcgc gatgcggcag tacccgagat tgtcggacca ctgcgcgcgc ggcacggaga
     2161 gcagcggggc gtcggcgcgc gtgcgctcga gcgccgagag gtacgcctcg gcggcctctt
     2221 cgaaccggct gtcggcggcg agcgcgtttc cgacttcgtt gaggcacgcg ccgatcaggc
     2281 gcgggtcccc gatctcctcg gcgtggcggc gcgcgaggtt cgcgtagaag aagaccttgt
     2341 tgcgctcctt gcggagcctg tagatcgcgg cgctcgaata cgcggcgcgg aacgccgtct
     2401 gaggctcgcg gttgcgcagg aggatccgct tcagctcgac gagctcgtcg gacgcgggtc
     2461 ccgtctcggc ggcggccgcg gcgcggcaga cgtagatcca gtcgacgaac gcgggctcgt
     2521 ccgtcgcccg ggccgacgcg agcgccgcgt cgtagcagtg gagtgcggcc gcgaactcgc
     2581 ccttctcgag gtgcgagagc gcttcgcggc gcgaggcctc ggcggccgag cgggcgtcca
     2641 cggccggctc agccattggt gtcgtctccg gggtcttcgc cggccttcgc cttctcgatc
     2701 tgcatcttct tgaaggcgat gaactcggcg acctcgcggc gggaatcctc ggggagcgcg
     2761 gcccagtcct cgaacggcgc ggcggctccg ggcgtcttcg ccgcggccgc cggctcggcg
     2821 gagggctcct cgaagaagtg gctcatggac agctcgaaca cctgaaggat ccggaagaga
     2881 gtgtcgagcc cgaccttgta ctcaccgttt tccatgcggg aaaggtcgga ctgtgtgacc
     2941 ccgatgcgcg cggcgagatc cgcctgggtc aggtgccggc tcttccggat ctgccggatc
     3001 ttctgcccca cgaggagaat ctgggacgcg cgcggaacgg cactcatggt cgctgaccgg
     3061 ttaagatagc ctttccgcgg tgccttttca agcccgtctt gcgccgcccg agggacgcta
     3121 gttcgttgaa gaacaaggtt ttcctgctct cccagctgct tcgcagggat ctcgccgccc
     3181 ggtacgccgg gagcttcgcg gggccgttct gggcgatcct gaacccggcg atcctgtgcg
     3241 gcctcaacgc cttcgtcttc gcgtacgtca tcaagctcag gccgccggcc ggcttcggcg
     3301 ggaccttcgt cgagttcctg ctggccggga tgctcccctg gctggggatc caggaagcga
     3361 tcgtgcgcgg aacgacctcc gtcacggacc aggcgcacct cgtcaagaag ctgaagttcc
     3421 cggtcgctct cctgaccctc tcctccgtcg gcgccgcact cgtgatccag acgatttcga
     3481 tcgccctcct gacgtgctac ctcctcgtcg cgggccgcgg ctcggtcgat ccgctgcttc
     3541 tcgccgcggc gttcggcttc gagctccttc tcctcgtggg cccggccctc atcctcgcgt
     3601 cgctcaaccc gttcttccgg gacctcgcgc agatcctccc gcccgtcctc atgatcgcgt
     3661 tctacgtgac gccgatcctc taccccgaat cgatgatgcc ggactgggcg gcgcccctcc
     3721 tcacgctcaa tccgctgcgc gacgtcatcg cgcttttccg cgcggcgctc ttcggaatgc
     3781 ctgccccgcc gctgcgccgt ctcggcatcg aggccgcggc attcatcgtg ctaggctttc
     3841 tgggacgtca gctctatcgc cggttgcgcc cggccttcgc ggacgttctc tgagccccga
     3901 atcccgctcg cccgccggcc gaaggccgcc gggttccgtg cgtcgacagg agacttcgtg
     3961 aaacccactt tcgccgtcat cgccgttgcg gccttttcgc tgctggcggg ctgctcttcc
     4021 tccacggctc cgaaaactgc ctccgcgccc gcccccgcac ccgacgcgaa aaagggcgct
     4081 ccggggcccg acgaggtcat gggtctccgg gtcccgggtc ccgtcgaagg cgaggacgag
     4141 cggaccctgc ccccgaagcc ctacttcgac ccggagttcg gaaagcgcgt cgtccggatc
     4201 ccgaagggcg accccatgtc gcagtactac gagaaggaag gccgcctctt tctcgcgatc
     4261 gtcgccgggc cgggcgttcc gatcctgcgc tcggacgccg acgcgtggat cgtggaggcg
     4321 ccgaaggagc gcaagatcgc cgaaaagagc gccaccgggg cgcccgaggc gccggtgatc
     4381 atcgaaatgc cgaagtccga gtcggaggcc gtcacgccga agacgtcgaa ggaaaccctc
     4441 cggttcgagg agatctcggg tgggcttccg cgctcgggca tgtggcggga gaacttcgac
     4501 ctcggcgacg tgctcggaac ggggcgcccg cagatcgtcg cgccgccggc gcggctgacc
     4561 ggaaagagcg tccaggtctt caggctcgac cgcgacgcgg cgggtgcgcc gcgttggcgg
     4621 gtcgtcgagc tcgagatgga aaacccggag aacgtcgacg cggcttacgg ggccgcgacg
     4681 gtcggtgaca tcgacggaga cggcaagctc gacatcgtct tcggcggcca cggctccggt
     4741 ccggcgatcg cgttcaacaa gggggacggc aagttccgca tcgagacgcg tggcctgccc
     4801 cggcagatgt ccacgcgggc catcgcggtc ggcgacctca acggggacgg caagctcgac
     4861 cttctcgcga tctcggacga gatggagtgg tcgaaggcgg gcgggaagcc ctcgttcgac
     4921 aaggactcga cgtacgagag gggatacgac gtccgggcct tcctgaacga gggcaagacg
     4981 ttcaaggacg tccacaaggg gacggagggc gcctgcttcg gttactcgat cgccctcgcg
     5041 gttccgaaga ccccggaagc cgggctgccg ttctacgcgt cggcctgccg gtatgtcggc
     5101 atcaacgcga tgctctacga gttcgacgcg aagaccgaga agttccgcga cctctggttc
     5161 ggcgtcgccg aggcgcacac gcagcacacg ggcgccgccg cgggcacgta ccgcgggcgg
     5221 ccggccgtgt tcggctcgtg gttcaagcgg tctcccttcg gggcgactcc gaagatcgac
     5281 ggcgaaggcg tgaccgccta ctaccgcggg acggacggaa agatggagac caagcgcgtc
     5341 gtcaagacgc tcaagttcgt cgcggcgtcc tatgcggtcg ccgccggcga cctgaacggc
     5401 gacggcctcg acgacgtcgt ctgggccgac gaggcggccg gacggatccg ggtcttcttc
     5461 cagacggccg agggggagtt cgaggagctg gcggcggatc gcgagccgac cttcatcaac
     5521 catccgacct gcctgcggat cgccgacgtg gacggagacg ggcgggcgga cgtcgtgctg
     5581 atgtaccagt acctcacggg cgacgagacc cgggccggcg gcttccgggt gttccgcggc
     5641 ctcggaaagt gagatttccg gggggtcgga cactagtccg atagatatgc gcccccaaca
     5701 tatttttcgg gattggcctt gacatcgggg aatttcgccc ctatcgtctg cccagtgttc
     5761 gggatatctc tatccgactc cgtgcttttc ttgcctcatg gcaatagcgg ctatgcaacc
     5821 gttgcttccg aaaaaattgc gggaggtgat tcaatatcgc aggcggcgag agcccggatt
     5881 tctcttctga agatcgtcga atcaacccgt atcaatcgaa ttcactggta ggagaaaaaa
     5941 tgaagaaatt ttccgttctg atcgcgctcg cgctgctcgc ggttgcgggc accggctacg
     6001 cggtcacgtg ctcgtacgac aacgtgccgg gcgcgacgct cctcgttcct tactggaagg
     6061 tctccctcaa cggcgccacg gacgcgccca tcccgacggg cggcatcgac accctcgtgt
     6121 cgatcgtcaa cgtttccaac cccggcgtga tcgctcacgt gacggtgtgg aacaagtact
     6181 ccaaggccgt catcgacttc aacctgccgc tcacgggcaa ggacgtcgcg tccttctcca
     6241 tgcgcgacat catgaacggc gcgctcagcc cgaacctgcc gcagtacaac accacgaagt
     6301 accccatcga cccgtgcggc ctcaagaacc ccggtctcgt caacgtcacg tacgttccga
     6361 gcatcggctg ggggcagacg cagttcatcc gcttctcgca ccctgaggcg acggccgccg
     6421 gtctcacgac cgacgtcctc cgctcgatct cgcagtacaa cctgacctcg accgacgcca
     6481 tcggctcggt gttccgtccc gtcgtgtggg actcgctcga cgagtcgggc gacatctcca
     6541 cgcacatctc ctccgcgggc gcgaacatcc tcgacaagga caacccgcag tgcggctggg
     6601 gctccagcga cggcacgtac tccggcgcgc tgtccggcta cctgacgatc gacgtcgtca
     6661 actactgcac gaactacttc cctgaccagg cccagttcta caacgaggac gcgatcgcga
     6721 cctccggctg gagccagtcc ggctacacgc cgaacgtcct catcggcgac gtgttctaca
     6781 tcgactccgc cacgaacggc ggcaacatct ccggcgaccc ggccatcccg gtcgagttcg
     6841 acgcccgcct caactggacg gccggcggcg gcacgaaggc caaccccgtc aagacgttct
     6901 tcggcaagta cgtgtgggac aaggccgaac cggcctgccc ggctgaggac ggcggcggct
     6961 gcgtgacggt caacgttccg gctccgttca tcttcggtgg cgacggccgc gagccgctcg
     7021 gcgaccgcta cggcttccgt tacctcgcga acgcctccgc gggctaccag acctggtcgc
     7081 tcgtgtggcg ctccgacctc tatgccacgg ccacgaccct gacctcgaac ctctgcacgt
     7141 gggcgaaccc gaactcgggc gcgaacaagg gcgcttcggg cacgggtctc tatgacgctc
     7201 tccaccaggt cacgtacgtc acgttcgaca acgacgagaa cgtgtacggc ggcgtcggca
     7261 tcccgccggg cccgtcgggc aacaacccgt cgcagaacac gatccagacg tacctcttcc
     7321 tcgagtccca gcgtatcgac ctgctcaagg cgaccgcgaa ggacgccgac tggaaccccg
     7381 cgcagttcac gggcggctgg gccgacctga cgctccgcgg cgcgacgatg accggcctca
     7441 cggcggccac gggcttctac aaccagggct gggtgggcgt gcagcacacc gcgcccggcg
     7501 ctgtcctcag cgtcggccac gcggctgcca gcctcagcaa ccagttccag tgctcgccgg
     7561 ccctcgtctt cggccccggc aacacgcctt aagttcctga cgtctcagga gaaacgggcg
     7621 gcttcggccg cccgtttttt tttcgaggga tgccccctga tgaggccact acggaagggc
     7681 acgggagatg caagtggggg tccgcctgct cgaaccgcgc cgcactggcg ggtgctagaa
     7741 ttgtatctct ttgtgtccga gccttttggg ggctcctccg caactacacg ggcctcgcgt
     7801 ccgagtcaca gggataggga atccagggaa tgaaacctct caggtccacg cgcaacgcgc
     7861 gcctcgtcac cggcgcattc gggctcgtcc tttcggcctt cctgctcgcg ggcgccccgg
     7921 cggcgctcgg ccaggggggc tgcatcgtct ccacgccgac gacgttcgag ccgaaccccc
     7981 cgggcccggc cgcgccgcgc aacgtgggcc ttctcagccc cgtcgcgttc aaccaccttt
     8041 ccctctacca gggcagctcg ggcgccgcgc gcatcctcat gcaggagacg ttcggctatt
     8101 cgacgctcga cctgacgaac ccggcccagc cgagcgtcct tcgctacgac tacttcccgt
     8161 tcgacacgaa caaggtcccg atcaacggcg acggccagag ctacacgcag gcgatcacgg
     8221 cgtcgtcgga cgggcagcgc gtcgcgctgt ccctgaatgg tccgattccg gcctgggcga
     8281 ataccgtcgt cggctatccc gacgggaaca acggctggac gctctggggc gacacggcgc
     8341 cggtcggcgg cgtcggcaac gcgatcctca agagcgggag ccgctacgtc cattacggga
     8401 tgacgtcctt tcagttctat gcgtcggacg tgacgaacca gcccgccacg ttcgcgggct
     8461 ccaacattcc cgcagagctg acggggctcc ccggtggagc gaggctctcg accgcgggca
     8521 gctacctcgt ctacacgacg gcgaccggcg acatccagat catcgacgcg tcgaaccccg
     8581 gccccgccgg gagcatcgcg tccggcctga agtggacgac gatcccgtcg tcctcgccga
     8641 tgttcaacgg ccactcgatc ggcgcgacga agtcggcgct cgagccgggg aacgcgaaga
     8701 tgctctggat cctcgccgag ctccagccgg cctccggcga gaactctccg agctacgcgc
     8761 tgttctcggt gacccaggac ctcgccggaa acttcggcac gcccgtcccc gcgggggccc
     8821 cgttccgcgt gccctccgtc gtcggagaca actggacgtc cgccggaaac tcgtcgtatc
     8881 tcgcggagca caacggcacg ctctacgtcc tcatgtgggc gttccggcgc acgccgagca
     8941 tccagtacgt cctccactcg accacgacga gcgcgtggac cacgggctcg ttcggcgttc
     9001 cgcagacgat cacggacccg acgttctccc tcggcgagat gaagacgctc gcgtcgggag
     9061 caaacctcta cgggtacatg gcgtccggga cctcggcctg ggtggtcccg atgacgtgca
     9121 cggccccgaa cgcgaaggcg acggccatcc tgaccgcggt gaacccggtc acgggcgtca
     9181 cggtcaattc gggtgacgcg gtcgtctacg gcgagacgct cacgttcgcg gcgcaggtgc
     9241 agccgacgcc gacgcagggc cagaactccg acctcgcggg cttccgctgg aacttcgact
     9301 tcgacttcca cacggggagc ctcgtcgagg acaacggggt cggcacgtcc ccccgcgtca
     9361 agaacaacga caacggcgag ttcgggaact ccgcgtaccc gccgtcgggg acgttcaacc
     9421 tcgtcggccc gtgcgacccg aagaacggcg gcgtgcgggg gacgggcacg ggctgctggg
     9481 cgtccgtcac gacgaacacg gcgcagggcg gcccggactt cacgggcagc gaggcggcgg
     9541 gcgccgtcaa gccgctcgtc gtcgccttcg aggcgaacaa cgcgaacggc agcaacgggg
     9601 cgaatctctt cacgatcaac tggaagcggc ccacgatcgg cctcgtctcg tctgcgatca
     9661 tcgccggcga ttcgctcgtc gcgtcgtacg acggccaccc gcagtcgcaa gacccgacct
     9721 ttccgtggaa gtggtacctc gacggggcgc tcgtgaactg caacacgggc tccgcgtgca
     9781 tcccggcggg cgccaacgcg actgccgggc agcacacgta ctgggtcacc gtgccgtacg
     9841 cgcagatcgg gtattccacg ccggattaca ccgggacgcc gatgggcacg tacacggtgt
     9901 cgacgttcgt cccgatcttc tccgtgaacg gctcggcgac gggccccgtg ggaacgaccc
     9961 cgagcggcac gctcacggtg gcgaacgcgt ccaagcgcgc caacaccaac atcaccggcg
    10021 cctactccta ctgcatcgtc cccgcggggc agtcgtgctc gagctacacg tcgtggtcga
    10081 tggcggaccc gacgaacacg aacggcagcc cggcgagctc ggccacgatc ccgaacccgg
    10141 gcgtgggcaa ctggatcctc aagatccggg tcgcctacac gggcggcacc gtcgactggc
    10201 cggacgcgaa tcccgcgaac gggatctcgc tcctgatcac gaacagcctc agcgtgtccg
    10261 cgtccgtttc gccgaacccg gcgaaccaca acgacacggt ggtcttccac tgctcggcga
    10321 cgggcggctc gggcaactac aactacaagt ggtccggcca gttcggcgac gtcgccttca
    10381 cccaggacta caccacccgc gtcacgaata cgggcggcca ggactcgagc ttctcgctgg
    10441 attgctccgc gacggacgcg acctcgggcg catccggatt cgccacggcc gcggtgacgg
    10501 tccaccccgg cgctccggcg ggagcgccga cggcatcggc gtcggtctca ccgaatccgg
    10561 tcgaggtggg taggccggcc acgttctcct gcacggggac gggtggcacc ggagccctca
    10621 cgtacaagtg gacggattct ctcttccacg agttctccgg tcagacgtac acctacatcc
    10681 cgacctcaca ggctcccgtc accttgacgt gtaccgtcac ggactccgcg aacagaaccg
    10741 gaacctcgtc ggcgacgctg ttcgtcgttc cgcccccggg ccccgcctgc ccggcggtgg
    10801 actggggcgt ctacgtcgac ggccaggagc tcctgccgac gcccggccag ttcggcctcg
    10861 agtttccgag gacggcgagc gtggggactc cgctcttctt ctcgatcaag tcggggatct
    10921 cgtcgcagtt cgagtggaac tggggagacg ggcaggtcac gacgctcacg ctggcccagt
    10981 accaggccaa cccgcccacg cacgcctaca cgaccacggg tctgaagacg attacgctca
    11041 ggccccagtt cgtggacgac agcggcttct gcggcggcag gtcctacgac atccagatca
    11101 cgggcccgaa cgccaacttc acgccgggct acctcgacgg ttcgccgttc gtcgtcgccg
    11161 acgtcacggc cggcaaggcc gtgcgtttct cggcgtcgga gggcggcgcg acctcgtacg
    11221 actgggactt cggcgacggg ctgcctcacg gctccggcca ggtcgtgacg cacatcttca
    11281 cgaccgcggg ggcccggacc gtcacgctca ccgtcgtcaa gggcgtcacc gtcagctcgt
    11341 cgctgacgct gacggtccac ctgccccccg agccgcccaa gtgggtcgtg ccgggcatgg
    11401 cgtacctcgc cggaagcggc ggcgcgttct ggacgtcgga cgtcacgatc ttcaaccccg
    11461 acaaggtcct cgcgcagaag ctctcggtcg cgttcctcga cccgaggaat ctcgtcacgg
    11521 actattcgca gctcacgttc gtgccgctcg cggacattcc gccgctgggt gccctggccg
    11581 gctcgaacgc gcttcaggcg ctcggcaagc ccgagggaag ctacggggcg cttctcttca
    11641 agggcaccct ggggcaggcg ctcgcgcccg tggtcgtgac ccggacgttc aacgaccagt
    11701 cttcgcaggg caagggcacg ttcggcctct cggtgccgag cgcgtcggcg gcgaccggcg
    11761 gggtcaccgc acaggcggcg cctgcggtgc agatgctcgt gggcctccgg caggatgcct
    11821 cggcccggac aaacctcgcg attgtgaacc tcaacaacga gtggccgacc gtcaagctcg
    11881 acctctacga cggccgcacg aactcgatcc tcggctccaa gacgctcccg ttgaacccgt
    11941 accaggtgct ccaggtggac cgggtcctcg agacggtcgc cggcgcgggc tcgacgagcg
    12001 acctcttcaa ggtgcaggtc tccgtcgtcg accagagcgg cggcgcgcac gcgtacgcgg
    12061 tctacccgta cgcgaccgtc atcgacgcgt actcgacgga cccgatcgtc gtcacgccga
    12121 cctcgacgcc ggccgccgcg taccggatcc cgggcatcgt ccgcctgacg ggcgcgaacg
    12181 gcgagaagtt ccggagccgg gtgaccgtgt cgaacccgtc gctggcgtcg cgcatcgtcc
    12241 ggatgacgta ctcgttcgtg tcctgtgacg cgagcggcgc atgcggcgat cgcaagacgt
    12301 actcgtccac cgtcggaatg gcgcccgggc agacgcaggc gtgggacgac tgggtcaagg
    12361 tctggttcca ggccaagtac gaagcaattc tcgacgaggc gaagaactac aaggactcgc
    12421 tcctcgacgt caccgccgac gacggcaaca cggacccgct cgtcgtcctc ggcgagacgt
    12481 actccgacct cggcaccgcc cccgacggca cgtcgcgcgg ccacgtcggg ctgcaggtgc
    12541 cgggctaccc ggccgccgac ggcgcgagcc gtgcgggctc gggtgcgaac ggaaacacgc
    12601 ggctcgtcct caccgggctc cggtccgacg cggcctaccg cacgaacctc gcgctcttcg
    12661 tgacgagcgg cacggcgggc ggctggtgcc gggtccgcgt gtacgcgccg gaaggcacgc
    12721 tgctcaagga cgtcccggtc gaggtccggc cggtcatgca ggtcagcggc gcgagcctcg
    12781 tggcggggat tccgggctcg ctgccccgct actccctcgt catcgacaac ttcgacgaag
    12841 ggatcgtcgt gggcggctac gcgacgatca tcgacaacgc gtcggccgac tcgacgttcg
    12901 tgaaggccct gcccgttccg tgaagccagg gcccgcccgg gcgcgtccgg gcgggctctt
    12961 ttccgcgcgt ccgggtcgcc gggacggcta acatcgcccg gagtcatgtc cgacgtgtcc
    13021 acgacctatt ccttcacgga cctctccaag gtctaccgcc tctattccgc gccgcgcgac
    13081 cgcctcgtcg aggccctcac gggccggccg cggcacgccg aagtccacgc gctccgcggc
    13141 gtgtcgggct cgatcgaccg gggcgcggtc ctcggcgtga tcggcgaaaa cggcgcgggc
    13201 aagagcacgc tcttcaagat cctcgcgggc acgaccgccg cgacgtccgg ctccgtgaac
    13261 ctcccggggc gcatcgcgtc catcctcgag ctgggctctt ccttccatcc ggacgacacc
    13321 ggccggagca acgtgatcct gcaggccgcg ctcgccggcc tctcgcgcga ggaggtttcc
    13381 gcggcgctcc ccgagatcga ggaattcgcc gagctcggcg acttcttcga ccgccccgtg
    13441 aagacgtact cgtcgggcat gcagatgcgc ctcgcgttct cggtcgcgac cgccgtcctg
    13501 cccgacgtga tcctcctcga cgaggcgctc gccgtgggag acggccgttt ccagaagaag
    13561 tgcgtcgacc ggatcttcga gctcaaggcc tccgggaaga cgattctctt ctgcagccac
    13621 gcgatgtact acgtctcgac gctctgcgac cgggcgatct ggctgaacgc gggcaaggtc
    13681 gcggccgaag gcgacgccca gtccgtggtg ctcgaatacg aacgctatct cgcgcgcaag
    13741 gactcggttt ccgccgcgca cgagacgccg ccgaatccct ccacgcacgg ccgcatcctc
    13801 ggcctctcct tccgcgactc cgcgggaaac ccggcggcgg agttccggcc gggcgagccg
    13861 tggatcgccg agatcgacgt cgaggccgac tctccggagc gtccgctcca ggtccacctg
    13921 ggcatctcga cgcgcgacag cgtcgtctgt ttctcggcgg attcgcgact cgacggcttc
    13981 gggcccttcc gcggtgcgag gcaataccgg ctccggatcg tcgtcgacgc gctcccgatc
    14041 gggaaggggg agttcgtcgc gactctgttc gtcggggacg agaacgcact cgcgctctac
    14101 gactcgcgca gcgccgtctt ccacgtggag tcctcgacgt ggcgatcggg cctgctcgcg
    14161 gtgcgccccc gctgggaatt ccgatcgtga tcgccgttct cgccgggccc gcgccggacg
    14221 gacgagcgga ccgcgccgcc ttcacgcgtt cgcacgatgc gtggcggggc gagagcgggc
    14281 cggatctcct cgtggtggag ccggacgacc cgcgcgcccc gtccgcggga ctccggctgg
    14341 acgtcgccga ggccgaggcg tccgtcgcgc cgggaggcct cgcgcgcgcc gccgcgatcc
    14401 ttctggcgag cgacgcgtcg tccgtggagc ccgcggggtt ctggcgcgga agccccagga
    14461 caccggagcc ctatgccacc gtctgggagt tcgaggaggt cgcggcgagg acgccgggcc
    14521 cgccttcctc cttgccttcc gattctccgg ccgctccggt cgcgctgcga cgtgcggagg
    14581 gaggggacgg atcgcgtctt tccacggagg cgtggaccgt ccattctttc gcgttccgcc
    14641 ggggtcacgc gcgtcccgag gtcgccgcgc tcgtgccgcc ggggagcgcg cgcatcctcg
    14701 agatcggctg cggcgaggga gcgctcggcg ccgtgctcga gagggcgggg gcgcgcgtca
    14761 caggagtcga gccggacgcc ggagccgcgc gcgtcgccgc gagccggctc tcgcgcgtcg
    14821 tgccgcgcgg gctggacgag gcgtgggacg agatcggcga gggctgggcc gccg
//
LOCUS       DAOMFY010000077        14814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1249_length_14814_cov_9.846602, whole genome shotgun sequence.
ACCESSION   DAOMFY010000077 DAOMFY010000000
VERSION     DAOMFY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14814
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_14814_cov_9.846602"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLB01_08225"
     CDS             complement(<1..883)
                     /locus_tag="PLB01_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQQ77329.1"
                     /translation="MRRVFMVGLAAIVSAGPGVVRAEGSESGRLTLGDAVRRAVEVSP
                     LMGAARGRVDAAAGAVRQAGRLPNPLGEVRVESWDFHADHATPRWDNVDFFATLAQPI
                     ELGGKRSARTAEAAAARDAAAFESEQTRHEVILETARTYLAAIRARSLLDAQQTNRET
                     LSRIVEILDRRVSEGWSAEADLLRFRAELARAEEALLRVRLDRDRELRFLAALLGEPS
                     AVAPARLVAPVLPPIPEGDADTLAHLGLARRPEVAAARARVEQAREASRLGRGRRVPP
                     RAPPAGYKRTVGLDTGKA"
     gene            1003..2667
                     /locus_tag="PLB01_08230"
     CDS             1003..2667
                     /locus_tag="PLB01_08230"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQQ77330.1"
                     /translation="MPAKPKTVKKPAPAKKDVFVYANPFPKGHDDTKYRLVAGPKGYV
                     RKAKFAGKTMLVVEPKALTVLAHEAMRDVSFLLRPKHQQQVASILSDPGASPNDRFMA
                     TTLLKNAVVSAQFELPFCQDTGTATVVAWKGQQVWTGADDAEKISKGIWQTYTQENLR
                     YSQTAALNMYDEVNTGNNLPAQIDIYAAKGDAYKFLFVAKGGGSANKTYLFQETKALL
                     NPASLETFLVEKMKTLGTAACPPYHIVFVVGGTSAEGCLKTVKLASTGYYDSLPTSGD
                     KYGRAFRDPELEKKLLERAQGLGFGAQFGGKYFAHDVRVIRLPRHGASCPVGMGVSCS
                     ADRNVLAKIDAKGLWLEELERHPENLLPVHTGAKEHAGIVKIDLNRPMKEILAELSKH
                     PVSTPLALTGRLVVARDIAHAKLKERLDSGQDLPQYFKDHPVYYAGPAKTPKGKPSGS
                     FGPTTAGRMDSYVDLFQSKGGSMIMIAKGNRSPQVTAACKKWGGFYLGSPGGPAAILA
                     QENIKKVEVVEYPELGMEAVWAIDVVDFPAFILVDDKGNDFFQKLG"
     gene            complement(2689..4032)
                     /locus_tag="PLB01_08235"
     CDS             complement(2689..4032)
                     /locus_tag="PLB01_08235"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQQ77331.1"
                     /translation="MRVHIRPATLVLSVALFAASPRVSGDFSDLESALRSEMEAKKIR
                     GAAMAVVRGDSVVFTAGLGDSDPRNLHPVTAGTFFRVGSTTKMFVATAALLLAHEGRL
                     DLDRPVSDYVAGLAPKVGRLTTNQLLTHTAGLFDDAPMAGPGDDGALGREVRSWTDEN
                     VFLPPGRFLSYSNPGYWLVGHVIETVAREPFADAMERLVLRPLGMAHSTFRPEAAARL
                     PVAAPFDGRRREISPAPNHAGTFPSGSLYSNVREMARLPIALMNGGRLEGLQVLPEEV
                     VAALLTPRVDIPERPGSRYGYGLIFETRRGVRQAFHTGGRAGYGSIVRMAPDQKVAVI
                     VFANRTSAVMGRTANAALGAVEAPGPASTAPSSKPFREGAADFEGTFECRKPLKLELY
                     RLGVLQVKHGFMRAPLRRRQDGLYTDSVLVLAATRGPGGRVDYLHTELHTLARVE"
     gene            4206..4664
                     /locus_tag="PLB01_08240"
     CDS             4206..4664
                     /locus_tag="PLB01_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77332.1"
                     /translation="MTKTATLLLAALVAAPALSQVPTPTKGARLPMTIKLDASALKAL
                     QLPPPMTTLHQFDEAVAAVKAQAENLKAAQKNFDDAERACATRTYSAQDMREAGCLDT
                     ETVAQCTVKLYDRCTRPSRRSYRLHHRNFITAVDALNQKARSLAIPPETP"
     gene            4729..4995
                     /locus_tag="PLB01_08245"
     CDS             4729..4995
                     /locus_tag="PLB01_08245"
                     /inference="COORDINATES: protein motif:HMM:NF022143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2492 family protein"
                     /protein_id="HQQ77333.1"
                     /translation="MSTPERVPATVIPGHDVLSLVAARGGHCTVAELKAAAALTFGAD
                     AVFGNCHGDLFDFPALLTFLEHKGKIARVGDEVTVGHVPACSGH"
     gene            5122..5661
                     /locus_tag="PLB01_08250"
     CDS             5122..5661
                     /locus_tag="PLB01_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ77334.1"
                     /translation="MREDFDREAWPHFREITRAALRLTRRPAEAEDLASEVFLQAWKS
                     FGKFERGTNCRAWLYKILWNVNQQRSRKKVPFTLGAEGEALLAETLAAEEPTPTEIKD
                     EEVSAALDALSPEHRQILLMSDVEEFTYKEIAAILRVPIGTVMSRLSRARAALREKVR
                     GTAAATSVAGNAAAQGGTP"
     gene            5658..6518
                     /locus_tag="PLB01_08255"
     CDS             5658..6518
                     /locus_tag="PLB01_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQQ77335.1"
                     /translation="MNVALNVRDYACAKVRRRLDSYLAGELSVDLSHEILEHLDRCPA
                     CAAELKARENLRGAVRRIAAATPEPREGFEAEVRARLARTPAPTTGNVTSLLLAASLV
                     AAAGAGTLFLLSRTSGPPATNLAAARDTKAFLFAALNHKNCALLFDKWSKVPAVSAAE
                     IGPKLDPELKAAAARAADRLPGYEAVTAHECNHAGEKVFHIIYRKSGSNSRNDLVSVI
                     ATRPGSTLASGVRLAGLVSGGDRDGLAVVGTSARDGRLVFLVTDGNDAEAMRLGKLVL
                     PALATAFAAP"
     gene            6515..7237
                     /locus_tag="PLB01_08260"
     CDS             6515..7237
                     /locus_tag="PLB01_08260"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HQQ77336.1"
                     /translation="MILAVLLAAASAPAPAPAATPVLVELFTSEGCSSCPPADRLLAR
                     LASGPAVPGALVVALSLHVDYWNRLGWEDPFSSHAFTERQGAYAIRFGSSGRVYTPQM
                     VVDGRTEFVGSDERAARRAIEAAAKEPRAFVRAVPAGARAVRVTVAGAGAGADVFLAV
                     VEDGLSSDVTRGENAGRTLPHAAVARTLASVGRVDPRGRFDAEVPVPPGSGARRAFAF
                     AQERGNGHVLGVSAPLTLAAAP"
     gene            7284..8162
                     /locus_tag="PLB01_08265"
     CDS             7284..8162
                     /locus_tag="PLB01_08265"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HQQ77337.1"
                     /translation="MRRALTLSFLAFAFLGGALCARADNPGAISGVHGHSDNLVSLSL
                     GVAPEIFPRYEGSDQSRTYWFPLVDLQIGRPNGPWLVYAGASRNYVPLGQGVGVYPVH
                     TGTFNWNVEIARTDTRPEDRGDALAGLGDRRANWFVSTRASWRFWHGHVQTDVALLHG
                     LLDDAGTLGYVRAELQGRTGPWFGAVASKVYVSDASNLRYDFGVTPEQAVTRAGLVAS
                     GDPRLTPSEIGPYLPGAGFRSADATASGGYAFAGRARLFAYGQLGVLLGPTVDSPLVK
                     TRRLYLVGAGVSVAVF"
     gene            8170..9201
                     /locus_tag="PLB01_08270"
     CDS             8170..9201
                     /locus_tag="PLB01_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQQ77338.1"
                     /translation="MPLTTVEVTYPRALGPVGLRGASPLSWSETAPPSSTRGSTRIFT
                     LDVPDGTTLDVKPVRGDGRFASGRNATVLAGETLRLSPYFEEAGVLDAAPRTLFSPQL
                     GRPVAFRVFLPPGYGELDARRYPVLYAQDGQSLFGAESWRLDETLDTLWGLGAMEEAI
                     VVAVHTDEGRLDMLSPTPDPGHGGGAGPRYRSFLTDTLKPYVDTFFRTRRGREDTALL
                     GSSMGGLFSFFAAWTRPDVFGKAACLSSSFWWDRRALVKDVAAGACPLPRPFLYIDSG
                     AARDPLAEDANLRDGYHHTMALRAALTGHCYEPGRNLHVLAFPGHAHDAASWGARLSV
                     PLQLLFPPR"
     gene            9235..11928
                     /locus_tag="PLB01_08275"
     CDS             9235..11928
                     /locus_tag="PLB01_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HQQ77339.1"
                     /translation="MSTNFSPVATLPPRAPAPTADPESLRLEEDRSRKVNWKRWGTYL
                     SERQWGTVREDYSPDGSAWAYFPHEHARSRAYRWGEDGLAGISDRRGLICFALALWNG
                     KDPILKERLFGLAGPEGNHGEDVKEQYYYLDATPTHSFMRMLYKYPQAEYPYTRLVEE
                     NARRGKGAPEFELVDAGAFDGGRYWDVSVAYAKGGPDDVLITVEIANRGPEAATLDVL
                     PTVWFRNTWSWGTDARKPRLRRANAPEGVRAVGLQHAYYGARTFYAEGAPELLFTENE
                     TNARRLFGVPNESPFVKDAFHEYVVHGVTEAVNPDGFGTKAAARYRVEVPAGGSRTIR
                     LRLTDRTFTEPFGAEFDRVFRTRRSEADLFYARIVPEKLSDDARSVMRQALAGMLWSK
                     QFYAYDVGRWLKGDPEHPAPPKERRAGRNAEWGHLYNADVISMPDKWEYPWYAAWDLA
                     FHAVPLALVDPDFAKQQLVLLLREWYMHPNGQLPAYEWKLSDVNPPVHAWAAWRVYKI
                     DAKRTGKPDRVFLERVFQKLLLNFTWWVNRKDEEGRNVFQGGFLGLDNIGVFDRSAPL
                     PTGGHIEQSDGTSWMGMYCLNMLAIALELSKDNPAYEDVASKFFEHFVYIARALNDLG
                     GEGIELWDKVDGFYYDVLHLPDGRHFPMRVRSMVGLIPLFAVETLEPETLDRLPGFKR
                     RLQWFLDNRPDFRQHVETVATDDGPRRFLSLVNRDRLRSVCRYLLDPEEFLSDYGIRA
                     LSRVHREKPYSLTVQGMEHRVDYEPAESSTGLFGGNSNWRGPIWFPVNFLLIEALQKF
                     HHFLGDGFTVEFPTGSGRRAHLWDVAADLSRRLVRIFLKDERGRRPVFGGTEIFQADP
                     NFSDHVLFYEYFHGDEGAGIGASHQTGWTGLVAKLIQQSGE"
     gene            11931..13106
                     /locus_tag="PLB01_08280"
     CDS             11931..13106
                     /locus_tag="PLB01_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQQ77340.1"
                     /translation="MTTLTETSPARPAADRATMKAIAVRPGVPDSVHLATLPVPSLAD
                     VPGGRGVLVRVLRVGVDGTDKEINAAEYGAAPEGSDVLVIGHEGFGVVEAVGENVTEV
                     APGDYVVATVRRPGSSPYDAIGLQDMTTDDTYFERGINLRHGFLSERYVDSADYIVKV
                     PPRLADVGVLLEPASVAQKAIFQAWEIQRRLRVWQPRRALVLGAGTLGLLASLFLRLR
                     GLSVVAMGRSERPYRNASLLDAIGVAYASARETTLQDVAAQHGPFDFILEGTGQSSLV
                     FEAMDVLGKNGVLAMVSVTGGGKTATVPTDRINQGFVLGNKLAFGSVNAHRDDFERGA
                     QDFARAELTWPGWLSRLLTHPVKGLESYRTLIDTLTKEKGAIKVYCDVAPLPGIPAA"
     gene            13103..14704
                     /locus_tag="PLB01_08285"
     CDS             13103..14704
                     /locus_tag="PLB01_08285"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQQ77341.1"
                     /translation="MKGGGTVADVTSAERGAEEGAGERLMAGAALGVPAWAIVSLVLL
                     PLLSGGAPQWDADAMRARLPQLTLWVLAGGAAGLLAHAAALLAPRISAGARVPAPAAA
                     PKRRVVILGGGFAGLQTAATLEHLLRGDPSVSITLVSETNALLFTPMLAEVAGSSLEA
                     SHISTPLRTSLGSAEFVRARVASIDVEGRRVLLASDAADAAKGMPVRELPFDHLVLAL
                     GSVSNYFGQRNVEEAAFDFKSLLDAIRIRNHVIEMFERADRESDAASRRALLTFVVAG
                     GGFAGVELAGALNDFCRGILADYPGLPPEELSVVLVHSRDRILPELSESLGRYARESM
                     EARGVTFRLQTRLVDARPGVVVLSDGELRAETLVWTAGVAPSPLLATLPFARDKRGAV
                     VVESTMAVPGQTGIWAVGDAAALMDGRTGKPCPPTAQFAIREGAHLARNIQAALSGKA
                     ASPFHFESLGALCVVGHQTACAELAVPFFPGRSVRFSGLLAWLMWRGIYLSKLPGLER
                     KVRVLVDWTIELFFPRDIVQTIELS"
BASE COUNT         2182 a         5488 c         4987 g         2157 t
ORIGIN      
        1 ccgccttccc cgtgtcgagg ccgaccgtcc tcttgtaacc ggcggggggg gcccgcggcg
       61 gaacgcgccg cccccgcccg agcctggacg cctcgcgtgc ctgctcgacg cgggcccgcg
      121 ccgcggcgac ctcgggccgc cgcgcgagcc cgaggtgcgc gagcgtgtcc gcgtcccctt
      181 ccggaatcgg cggaagaacg ggcgcgacga gccgcgccgg agcgacggcg gacggctctc
      241 cgaggagcgc ggcgagaaac cggagttcgc gatcacgatc gagccggacc ctcaggagcg
      301 cttcttcggc gcgggccagc tcggcccgga acctgagaag gtccgcctcg gccgaccagc
      361 cttccgagac gcggcggtcc aggatttcga cgatccggga gagcgtctcg cggttcgttt
      421 gctgcgcgtc caggagggac cgtgcgcgga tcgccgccag gtacgtacgc gcggtctcga
      481 ggatcacctc gtggcgcgtc tgctctgact cgaaggcggc ggcgtcccgc gcggccgcgg
      541 cctcggcggt ccgcgccgag cgcttccctc cgagctcgat cggctgcgcg agcgtcgcga
      601 agaagtccac gttgtcccag cgcggcgtcg cgtggtcggc gtggaagtcc cagctttcca
      661 cacgcacctc gccgaggggg ttcggaagac ggcccgcctg ccgaacggcg ccggccgcgg
      721 catcgacccg gccgcgcgcc gcgcccatga ggggcgagac ctccacggcg cgccgaacgg
      781 cgtcgccgag ggtgaggcga ccggactcag aaccttcggc ccgcaccacg ccgggccccg
      841 cgctcacgat cgcggcgagc ccgaccatga agacacgtcg cacaaaccag aatgtcaggc
      901 ccgccgctcc cactgtcaac gggcgatgcc ccgacttggt tcatccgcat cagaggcgac
      961 ccctgccggg gtcgggtagt ttccacgctg gaggagactc cgatgcccgc gaagccaaag
     1021 acggtgaaga agcccgcccc cgcgaagaag gacgtcttcg tgtacgcgaa cccgttcccg
     1081 aagggccacg acgacacgaa gtaccgcctc gtcgccgggc cgaagggcta cgtgaggaag
     1141 gcgaagttcg ccggcaagac gatgctcgtc gtcgagccga aggcgctcac ggttctcgcg
     1201 cacgaggcca tgcgcgacgt ctcgttcctc ctccgcccga agcaccagca gcaggtcgcg
     1261 tcgatcctgt ccgaccccgg cgcgtcgccg aatgacaggt tcatggcgac gacgctcctc
     1321 aagaacgccg tcgtctcggc gcagtttgag ctcccgttct gccaggacac gggcacggca
     1381 accgtcgtcg catggaaggg ccagcaggtg tggacgggcg ccgacgacgc cgagaagatc
     1441 tcgaagggca tctggcagac gtacacgcag gagaacctgc gctactcgca gacggccgcg
     1501 ctgaacatgt acgacgaggt caacacgggc aacaacctcc ccgcgcagat cgacatctac
     1561 gcggcgaagg gcgacgcgta caagttcctg ttcgtcgcga agggcggcgg ctcggcgaac
     1621 aagacgtacc tcttccagga gacgaaggcg ctcctgaacc ccgcgagcct cgagacgttc
     1681 ctcgtcgaga agatgaagac gctcggcacg gccgcatgcc cgccgtacca catcgtgttc
     1741 gtcgtgggcg gcacgtccgc cgagggttgc ctgaagaccg tcaagctcgc ctcgacgggc
     1801 tactacgact ccctgccgac gtccggggac aagtacggcc gggcgttccg cgaccccgaa
     1861 ctcgagaaga agctcctcga acgggcgcag ggcctcggct tcggcgccca gttcggcggc
     1921 aagtatttcg cgcacgacgt gcgcgtcatc cgcctcccgc ggcacggcgc gtcctgcccg
     1981 gtcgggatgg gcgtctcgtg ctcggccgac cgcaacgtcc tcgcgaagat cgacgcgaaa
     2041 gggctgtggc tcgaggagct cgagcgccac cccgagaacc tcctcccggt tcacaccggt
     2101 gcgaaggagc acgcgggaat cgtgaagatc gacctgaaca ggccgatgaa ggagatcctc
     2161 gcggagctga gcaagcatcc cgtgtcgacc ccactggccc tgaccggccg tttggtcgtc
     2221 gcgcgcgaca tcgcgcacgc gaagctcaag gagcgcctcg actccgggca ggacctcccg
     2281 cagtacttca aggaccaccc cgtctactac gcggggcccg cgaagacgcc gaagggcaag
     2341 ccgtccggat cgttcgggcc gacgaccgcc ggccgcatgg actcgtacgt cgacctcttc
     2401 cagagcaagg gcgggtcgat gatcatgatc gcgaagggca accgctcgcc acaagtcacg
     2461 gcggcctgca agaagtgggg cggcttctac ctcgggtcgc ccggcggccc ggcggccatc
     2521 ctcgcgcagg agaacatcaa gaaggtcgag gtcgtcgaat accccgagct cgggatggag
     2581 gcggtctggg cgatcgacgt cgtcgacttc cccgccttca tcctcgtgga cgacaaggga
     2641 aacgacttct tccagaaact cggctgaacg ccggcggctc cgcgcggcct attccacccg
     2701 cgcgagcgtg tggagctcgg tgtgaaggta gtcgacgcgc ccgcccggac cgcgcgtcgc
     2761 cgcgaggacg aggacgctgt ccgtgtagag cccgtcctgc cggcgccgga gcggcgcccg
     2821 catgaagccg tgcttcacct gcaggacgcc cagtctgtag agctcgagct tcaacggctt
     2881 tcggcactcg aacgtgccct cgaagtccgc ggctccctcc cggaagggct tcgacgacgg
     2941 ggcagttgaa gcaggaccgg gggcctcgac ggccccaagc gcggcgttcg ccgttcggcc
     3001 catgaccgcg gacgtgcggt tcgcgaagac gatcaccgcg accttctgat ccggcgccat
     3061 ccgcacgatc gagccatatc ccgcccgacc cccggtgtgg aaggcctgcc tgacaccgcg
     3121 ccgcgtctcg aagatgagtc cgtagccata acggcttccc ggccgctcgg ggatgtccac
     3181 gcggggggtg aggagtgcgg ccacgacctc ctccggcagg acctggagac cctcgaggcg
     3241 gcccccgttc atcagcgcga tgggaaggcg cgccatctcg cgcacgttgg agtagagcga
     3301 acccgagggg aacgtgccgg cgtggttcgg agccggagag atctcgcggc ggcggccgtc
     3361 gaagggcgcc gccacgggaa gccgggccgc ggcctcgggc ctgaacgtgg aatgcgccat
     3421 cccgagcggc ctcaggacga gacgctccat cgcgtcggcg aacggctcgc gtgccacggt
     3481 ctcgatcacg tgcccgacga gccagtagcc gggattcgaa taggagagga accggcccgg
     3541 cgggaggaag acgttctcgt ccgtccagga acgcacctcg cgcccgagcg cgccgtcgtc
     3601 gccgggtccc gccatcggcg cgtcgtcgaa gaggcccgcg gtatgcgtca ggagctggtt
     3661 cgtcgtgagc cgcccgactt tcggggcgag ccccgcgacg tagtcggaga cgggccggtc
     3721 caggtcgagg cgtccctcgt gcgcgaggag aagcgccgcc gtcgcgacga acatcttcgt
     3781 cgtcgagccg acgcgaaaga acgtccccgc cgtgaccgga tggagattcc gagggtccga
     3841 atcgccgagg cccgccgtga agacgacgga gtcgccgcgg accaccgcca tcgcggctcc
     3901 gcggattttc ttcgcctcca tctccgaccg gagcgcgctc tcgaggtcgc tgaagtcccc
     3961 ggagacccgc ggggatgcgg cgaagagcgc taccgacagg accagcgtcg cgggccgaat
     4021 gtggactctc attccctccc gaacggcccg aacggaaaaa ggttcggcaa atccgcggct
     4081 ggtctcgaaa cgcagatcgc tggcctctcg acgccagatc gatccgcgcg tgagcaacgt
     4141 cacgcccccc ggcgtgcttt gcgctccccc gtgccgtaga ttcgtgtgcg aaggaggctc
     4201 cgtccatgac gaagaccgca accctgcttc tcgccgcgct cgtcgctgcc cccgccctgt
     4261 cgcaggttcc gacgccgacg aagggagcaa ggctgccgat gacgatcaag ctcgacgcgt
     4321 ccgcattaaa ggcccttcag ctcccgccgc cgatgaccac gctccaccag ttcgatgagg
     4381 cggtcgcggc cgtgaaggcg caggccgaga acctcaaggc cgcgcagaag aacttcgacg
     4441 acgcggagcg cgcgtgcgcg actcgcacct actcggcgca ggacatgcgc gaagcgggct
     4501 gcctcgacac cgagaccgtc gcgcagtgca cggtcaagct ctacgaccgg tgcacgcgtc
     4561 cctcgaggcg ttcataccgc ctccatcacc gcaacttcat cacagccgtc gacgctctca
     4621 atcagaaggc gcgctcgctc gcgatcccgc ccgagacacc ctgaccgccg cgcccccgta
     4681 tgacgccggt catacggggg ctgtctccga ggtgctacct tgtcgcgcat gtcgactcct
     4741 gaacgcgtcc ccgcgaccgt catcccgggc catgacgtcc tgtccctcgt tgccgcgcgc
     4801 ggcgggcact gcacggtcgc cgagctgaag gccgccgccg ccctcacctt cggcgcggac
     4861 gccgtcttcg gcaactgcca cggcgacctc ttcgacttcc ccgcgctgct caccttcctc
     4921 gagcacaagg gcaagatcgc ccgcgtcggg gacgaggtga ccgtgggcca cgtccccgcc
     4981 tgctcgggcc actgacgaag agacggacgc ggggagcacg ggcgcccggg gaataatgcc
     5041 ggttcgacgc ggcatatacg cccggaggcc accgcccgca tgtccctgtt ccgtccacgc
     5101 ccggccgacg cggagtcccg catgcgcgag gacttcgacc gcgaggcctg gccgcacttc
     5161 cgggagatca cgcgggcggc gctgcgcctg acgcggcgcc ccgccgaagc cgaggacctc
     5221 gcctccgagg tcttcctgca ggcctggaag tcgttcggga agttcgagcg cggcacgaac
     5281 tgccgcgcat ggctttacaa gatcctctgg aacgtgaacc agcagaggag ccgcaagaaa
     5341 gtccccttca cgctcggcgc cgagggggag gctcttctcg ccgagacgct ggccgccgag
     5401 gagccgaccc ccaccgagat caaggacgag gaggtttcgg ccgcgctcga cgccctcagc
     5461 cccgagcacc ggcagatcct tctgatgtcc gacgtcgagg aattcaccta caaggaaatc
     5521 gccgccatcc tccgcgtgcc gatcggcacg gtcatgtcgc gcctgtcccg ggcgcgcgcc
     5581 gcgctgcggg agaaggtgcg cgggacggcc gccgcgacga gcgtcgcggg gaacgcggcc
     5641 gctcaaggag ggaccccgtg aatgtcgccc tgaacgtccg ggactacgcc tgcgccaagg
     5701 tgcgccggcg gctggactcg tacctcgccg gcgagctgtc cgtcgacctc tcgcacgaga
     5761 tcctcgagca tctcgaccgc tgccccgcgt gcgccgccga gctgaaggcc cgcgagaacc
     5821 tgcgcggcgc cgtccgccgc atcgccgccg ccacgcccga gccccgcgag ggcttcgagg
     5881 ccgaggtccg cgcgcgcctt gcgcggactc cggctcccac gaccggcaac gtcacctcgc
     5941 ttctcctcgc cgcgtcgctc gtggcggccg ccggggcggg aacgctcttc ctgctctccc
     6001 gcacgtcggg accgccggcg accaacctcg cggccgcccg cgacacgaag gcgttcctct
     6061 tcgccgccct caaccacaag aactgcgcgc tgctcttcga caagtggtcg aaggtccccg
     6121 ccgtctccgc cgcggagatc gggccgaagc tcgacccgga gctgaaggcc gccgccgccc
     6181 gggcggcgga ccgcctgccc ggctacgagg cggtcaccgc gcacgaatgc aatcacgccg
     6241 gcgagaaggt gtttcacatc atctatcgca agagcggctc caattccagg aacgacctcg
     6301 tctcggtaat cgcgacgcgg ccgggatcga cgctggcctc gggcgtgagg ctcgccggcc
     6361 tcgtcagcgg cggcgaccgc gacggcctcg ccgtcgtcgg gacgagtgca cgggacggcc
     6421 gcctcgtctt cctcgtcacg gacgggaacg acgcggaggc catgcggctc ggcaagctcg
     6481 tcctgcccgc gctcgcgacg gcgttcgccg cgccgtgatc ctcgcggtgc tcctggccgc
     6541 ggcatccgcg ccggcgcccg cgccggccgc gacgccggtc ctcgtggagc tcttcacgtc
     6601 ggagggctgc tcgagctgcc cgcccgcgga ccggctgctc gcacgactgg cctccgggcc
     6661 ggccgtgccc ggcgccctcg tcgtcgcgct ctcccttcac gtcgactact ggaaccgcct
     6721 cggatgggag gatcccttct cctcgcacgc gttcacggag cgccagggcg cctacgcgat
     6781 ccgcttcggc tcctccgggc gcgtctatac accgcagatg gtcgtggacg ggcggacgga
     6841 attcgtcgga agcgacgagc gcgccgcacg gcgcgcgatc gaggctgcgg cgaaggagcc
     6901 gcgcgccttc gtccgcgccg ttcccgcggg agcgcgggcc gtgcgcgtga ccgtcgcggg
     6961 cgccggggcc ggggccgacg tcttcctcgc cgtcgtggag gacggcctct cctctgacgt
     7021 cacgcgaggt gagaacgcgg ggcggacgct tccccatgcc gccgtcgccc ggacgctggc
     7081 ctccgtgggt cgggtggatc cccgcgggcg gttcgatgcc gaggtccccg ttccgccggg
     7141 ctcgggcgcg cgccgcgcgt tcgcgttcgc gcaggagcgc gggaacgggc acgttctcgg
     7201 cgtttccgcc ccgctcacgc tcgccgccgc gccctgaagc ttcttcggaa tcttcttcgg
     7261 tcgctccgac atctatcgtc gtgatgcgac gcgctctcac cctctcgttc ctcgccttcg
     7321 ccttcctggg cggagctctt tgcgcccgcg cggacaaccc cggggcgatc tcgggcgtgc
     7381 acggccactc ggacaacctc gtgtccctga gcctcggcgt cgcgcccgaa atcttcccgc
     7441 gctacgaggg ctcggaccag agccgcacgt actggttccc gctcgtggac ctccagatcg
     7501 ggcgcccgaa cggcccgtgg ctcgtctacg caggagcgag ccgcaattac gtcccgctcg
     7561 gccagggcgt gggcgtctac cccgtccaca cggggacgtt caactggaac gtggagatcg
     7621 cgcggacgga cacgcgcccc gaggaccgcg gcgacgcgct cgcgggcctc ggcgaccggc
     7681 gtgcgaactg gttcgtctcg acgcgcgcct cgtggcggtt ctggcacggc cacgtccaga
     7741 ccgacgtcgc gctcctgcac ggcctcctcg acgacgcggg gacgctcggc tacgtccgcg
     7801 ccgagctgca gggaaggacc gggccgtggt tcggcgcggt cgcctccaag gtctacgtct
     7861 cggacgcctc gaacctgcgc tacgacttcg gcgtcacgcc ggagcaggcc gtgacgcgcg
     7921 ccgggctcgt cgcctcgggc gacccgaggc tgacgccgtc ggagatcggg ccgtacctgc
     7981 ccggcgcggg cttccgcagc gcggacgcga cggcgagcgg cgggtatgcg ttcgcagggc
     8041 gcgcccgcct cttcgcctac ggccagctcg gcgtccttct cggacccacg gtcgacagcc
     8101 cgctcgtgaa gacgcgccgc ctctacctcg tcggcgccgg cgtgagcgtc gcggtgttct
     8161 gagaaaccga tgccgctcac gacggtagag gtcacgtacc cgcgcgcgct cggccccgtc
     8221 ggcctgcgcg gcgcttcgcc gctctcgtgg agcgagacgg cgccgccgtc gtccacgcgg
     8281 ggctcgacgc ggatcttcac gctggacgtg ccggacggga cgaccctgga cgtcaagccg
     8341 gtgcgcgggg acggtcgttt cgcgagcggt cgcaacgcga ccgtcctcgc gggcgagacg
     8401 ctgcgcctct cgccgtactt cgaggaggcg ggcgtcctcg acgcggcgcc gcggacgctc
     8461 ttctcgccgc agctcggccg gccggtcgcg ttccgcgtct tcctcccgcc cggttacggc
     8521 gagctcgacg cgcgccgcta tcccgtcctc tacgcgcagg acgggcagtc gctcttcggc
     8581 gcggagagct ggaggctcga cgagacgctc gacaccctct ggggcctcgg ggccatggag
     8641 gaggcgatcg tcgtcgccgt ccacacggac gaagggcgcc tcgacatgct ctccccgacc
     8701 ccggatcccg gtcacggcgg cggcgccggg ccacgctatc ggtcctttct gacggatacc
     8761 ctcaagccct acgtggacac gttcttccgg acgcgccgcg gccgcgagga cacggccctc
     8821 ctcggctcct cgatgggcgg gctcttctcg ttcttcgccg cgtggacgcg ccccgacgtc
     8881 ttcgggaagg cggcatgtct ctcgagctcc ttctggtggg accggcgcgc gctcgtgaag
     8941 gacgtcgcgg cgggcgcctg cccgcttccg cggccgttcc tctacatcga cagcggggcg
     9001 gcgcgggacc cgctcgccga ggacgcgaac ctgagggacg gctatcacca cacgatggcg
     9061 ctccgcgcgg ccctcacggg gcactgctac gagcccggcc ggaaccttca cgtcctcgca
     9121 tttccggggc acgcgcacga cgccgcgtcg tggggcgcgc ggctctccgt ccccctccag
     9181 ctcctcttcc cgccgcgctg aaacgcccgt cgcgctgcgg catcgaaaag acagatgtcc
     9241 acgaacttct cccctgtcgc gaccctcccg ccgcgcgccc cggcgcccac ggccgatccc
     9301 gagagccttc gcctcgagga agaccggtcc cgcaaggtca actggaagcg ctggggtact
     9361 tacctctccg agcgccagtg gggcacggtc cgcgaggact acagcccgga cggctcggcc
     9421 tgggcgtact tccctcacga gcacgcgcgc tcgcgggcgt accgatgggg cgaggacggg
     9481 ctcgcgggaa tctcggaccg gcgcggcctg atctgcttcg ccctcgcgct ctggaacggg
     9541 aaggacccga tcctcaagga acgcctcttc ggtctcgcgg gtcccgaggg aaaccacggc
     9601 gaggacgtca aggagcagta ctactacctc gacgcgacgc cgacgcactc cttcatgcgg
     9661 atgctctaca agtacccgca ggccgaatat ccgtacacgc gactcgtgga ggagaacgcg
     9721 cgccgcggca agggcgctcc ggagttcgag ctcgtcgacg cgggggcgtt cgacggcggc
     9781 cggtactggg acgtctccgt ggcgtacgcc aagggaggcc cggacgacgt tctgatcacc
     9841 gtcgagatcg cgaaccgcgg gcccgaggcc gcgacgctcg acgtcctccc gaccgtctgg
     9901 ttccggaaca cgtggtcctg gggaacggac gcgcgcaagc cccgcctcag gcgggcaaac
     9961 gcaccggaag gcgtccgggc cgtcgggctc caacacgcgt actacggggc gcgcacgttc
    10021 tatgccgagg gggcgcccga gcttctcttc acggagaacg agacgaacgc gcgccgtctc
    10081 ttcggtgtgc cgaacgagag cccgttcgtc aaggacgcgt tccacgaata cgtcgtccac
    10141 ggcgtgaccg aggcggtgaa cccggacggc ttcgggacga aggccgccgc gcgctaccgc
    10201 gtcgaagtcc cggcaggagg gtcgcggacg atccgcctgc gcctcacgga ccggactttc
    10261 accgagccgt tcggagcgga gttcgaccgc gtctttcgga cccgccggag cgaggccgat
    10321 ctcttctatg cccgcatcgt cccggagaag ctctccgacg acgcccggag cgtgatgcgg
    10381 caggcgctcg cgggaatgct ctggtccaaa cagttctacg cctacgacgt cggccgctgg
    10441 ctcaagggcg atcccgagca tccggcgccg ccgaaggaac gccgcgccgg gcggaacgcc
    10501 gagtggggac acctctacaa cgcggacgtc atctcgatgc ccgacaagtg ggagtacccg
    10561 tggtacgccg cctgggacct cgcgttccac gccgtgccgc tcgcgctcgt ggacccggac
    10621 ttcgcgaaac agcagctcgt cctcctcctg cgcgagtggt acatgcaccc gaacggccag
    10681 ctcccggcct acgagtggaa gctctcggac gtgaacccgc ccgtccacgc gtgggccgcc
    10741 tggcgcgtct acaagatcga cgccaagcgc accggcaagc ccgaccgtgt cttcctcgag
    10801 agggtcttcc agaagctcct cctgaacttc acgtggtggg tgaaccgcaa ggacgaggag
    10861 ggccgcaacg tattccaggg cggtttcctc ggcctcgaca acatcggcgt cttcgaccgc
    10921 tccgcgccgc tgccgacggg cgggcacatc gagcagtccg acggcacgag ctggatgggc
    10981 atgtactgcc tcaacatgct cgcgatcgcg ctcgagctct cgaaggacaa cccggcctac
    11041 gaggacgtcg cgtcgaagtt cttcgagcac ttcgtctaca tcgcccgcgc cctgaacgac
    11101 ctcggcggcg aggggatcga gctgtgggac aaggtggacg ggttctacta cgacgtcctg
    11161 cacctgcccg acggccgcca ctttccgatg cgcgtgcgct cgatggtcgg cctgatcccg
    11221 ctcttcgccg tcgagacgct cgagcccgag acgctcgacc gcctgccggg cttcaagcgc
    11281 cgtctccagt ggttcctcga caatcgcccc gacttccgcc agcacgtcga gacggtcgcg
    11341 acggacgacg gcccgcgccg cttcctctcg ctcgtgaacc gcgaccggct gcgctccgtc
    11401 tgccgctacc tcctcgaccc cgaggagttc ctctccgact acgggatccg cgcgctctcg
    11461 cgcgttcacc gcgagaagcc gtattccctc acggtccagg ggatggagca ccgcgtcgac
    11521 tacgagcccg ccgagtcgtc caccggcctt ttcggcggca actccaactg gcgcggcccg
    11581 atctggtttc ccgtgaactt cctcctcatc gaggccctcc agaagtttca ccacttcctc
    11641 ggcgacggct tcaccgtcga gttcccgacg ggctcgggcc ggcgcgcgca cctctgggac
    11701 gtcgcggccg acctctcgcg ccggctcgtg cggatcttcc tgaaggacga gagaggccgg
    11761 agaccggtct tcggcgggac cgagatcttc caggccgacc cgaacttctc ggaccacgtc
    11821 ctcttctacg agtacttcca cggcgacgag ggcgcgggca tcggcgcgtc ccaccagacg
    11881 ggttggaccg gcctcgtcgc gaagctcatc cagcagtccg gagaatgaga atgaccacgc
    11941 tcaccgaaac gtctcccgcc cgcccggccg cggaccgcgc cacgatgaag gcgatcgccg
    12001 tccgcccggg cgtccccgat tcggtccacc tcgcgacgct gcccgtgccg tccctcgccg
    12061 acgtgcccgg cggccgcggc gtcctcgtga gggtcctccg cgtgggggtc gacgggacgg
    12121 acaaggaaat caacgcggcc gaatacggcg ccgcgcccga gggctcggac gtcctcgtga
    12181 tcggccacga gggcttcggt gtcgtggagg ccgtcggaga gaacgtgacg gaggtcgcgc
    12241 ccggcgacta cgtcgtcgcg accgtgcggc gcccgggatc cagcccctac gacgcgatcg
    12301 gcctgcagga catgacgacc gacgacacgt acttcgagcg cgggatcaac ctccgccacg
    12361 ggttcctgtc cgagaggtac gtcgactcgg ccgactacat cgtcaaggtt ccgccgcgcc
    12421 tcgcggacgt cggcgtcctg ctcgagcccg cgagcgtcgc ccagaaggcg atcttccagg
    12481 cctgggagat ccagcgccgc ctgcgcgtct ggcagccgag gcgcgcgctc gtcctgggcg
    12541 cgggcacgct cggcctcctg gcctcgctct tcctgcgcct gcgcggcctc tccgtcgtcg
    12601 cgatgggccg gtcggagcgc ccgtaccgca acgcgagcct cctcgacgcg atcggcgtcg
    12661 cgtacgcgag cgcacgggaa accacgctcc aggacgtcgc cgcccagcac ggccccttcg
    12721 acttcatcct cgaggggacg ggccagtcct ccctcgtctt cgaagccatg gacgtcctcg
    12781 gcaagaacgg cgttctcgcg atggtgagcg tcacgggcgg cggaaagacg gccacggtcc
    12841 cgacggaccg catcaaccag ggcttcgtcc tcgggaacaa gctcgccttc ggcagcgtga
    12901 acgcccatcg cgacgacttc gagcgcgggg cgcaggactt cgcgcgcgcc gagctcacgt
    12961 ggccgggctg gctctcgcgc ctcctcacgc atcccgtgaa gggcctcgag agctaccgga
    13021 ccctcatcga cacgctcacg aaggagaagg gcgcgatcaa ggtctactgc gacgtggctc
    13081 ctcttcccgg aatccccgcg gcgtgaaagg cggcgggacg gtggcggacg tgacgtcggc
    13141 ggagcgcggc gccgaagagg gcgcgggcga gcggctcatg gccggggcgg cgctcggcgt
    13201 gcccgcgtgg gcgatcgtca gcctcgtcct tcttcccctc ctttcgggcg gcgcgccgca
    13261 gtgggacgct gacgccatgc gcgcccgcct tccgcaactc accctctggg tcctcgcggg
    13321 cggcgccgcc ggtctcctcg cccacgccgc cgccctcctt gcaccgcgga tctccgccgg
    13381 cgcacgcgta ccggcgcccg cggcggcccc gaagaggcgc gtcgtcatcc tcggcggggg
    13441 gttcgcgggc ctgcagaccg cggcgacgct ggagcatctt ctgcggggtg atccgtcggt
    13501 gtcgatcacg ctcgtcagcg agacgaacgc gctcctcttc acgcccatgc tcgccgaggt
    13561 ggccggctcg agcctcgagg cgagccacat ctcgacgccg ctccgcacga gcctcggctc
    13621 ggccgagttc gtgcgggcgc gcgtcgcctc gatcgacgtc gaggggcgcc gcgtgctcct
    13681 cgcgtccgac gccgccgatg cggccaaggg catgcccgtc cgcgagcttc ccttcgatca
    13741 cctcgtcctc gcgctcggat cggtctcgaa ctacttcgga cagaggaacg tcgaggaagc
    13801 cgccttcgac ttcaagagcc tcctcgacgc gatccggatc cgcaaccacg tcatcgagat
    13861 gttcgagcgg gccgaccgcg agagcgacgc cgcttcccgt cgcgcgctcc tcacgttcgt
    13921 cgtcgcgggc ggcggcttcg ccggcgtcga gctcgccggg gcgctgaacg acttctgccg
    13981 cgggatcctc gcggactatc cgggcctccc gcccgaggag ctgtccgtcg tgctcgtcca
    14041 ctcgcgcgac cggatcctcc cggagctctc cgagtcgctc ggccgctacg cgcgggagag
    14101 catggaggcg cgcggcgtga cgttccgcct ccagacccgt ctcgtggacg cgcgccccgg
    14161 cgtggtcgtc ctctccgacg gcgagcttcg ggccgagacg ctcgtctgga cggccggcgt
    14221 cgccccgagc ccgctcctgg cgaccctccc gttcgcgcgc gacaagcgcg gcgccgtcgt
    14281 cgtggagtcg acgatggccg tccccgggca gaccgggatc tgggccgtcg gcgacgcggc
    14341 cgcgctgatg gacggccgga ccggcaagcc ctgccccccg acggcgcagt tcgcgatccg
    14401 cgagggggcg cacctcgccc ggaacatcca ggcggctctc tcgggaaagg ccgcaagccc
    14461 cttccacttc gaatcgctcg gcgcgctctg cgtcgtcggc caccagacgg cctgcgccga
    14521 gctcgcggtg ccctttttcc ccggaaggtc cgtgcgcttc tcgggcctcc tcgcctggct
    14581 catgtggagg ggcatctacc tctcgaagct ccccgggctc gaacggaagg tccgcgttct
    14641 cgtggactgg acgatcgagc tcttctttcc gcgggacatc gtccagacga tcgagctttc
    14701 atgacggata acaagcgctc cctcccggtc ctcgccgccg ctctcgtggg ggccgtcggc
    14761 gcggccgtcg ccgcggccgc gctcggcgcc tccctcgcgc ctgccgcgct cctc
//
LOCUS       DAOMFY010000078        14690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1260_length_14690_cov_8.729142, whole genome shotgun sequence.
ACCESSION   DAOMFY010000078 DAOMFY010000000
VERSION     DAOMFY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14690
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1260_length_14690_cov_8.729142"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLB01_08290"
     CDS             <1..858
                     /locus_tag="PLB01_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ77342.1"
                     /translation="APAAAVPAVAQPFKDVGLWYGVFGTTICSWFDAGDGVFLVDTGS
                     TAADAKALRAEIAKTTKNKPVKWIAMTHLHTDSNEGLATFFPADVTIFVNARSAATLA
                     DFAGRQKSPTPNVVGVADRIVLGLGKLRLEIGVPPGNAHTGSDLYVFDAVTRTAFVGD
                     LVTPDRCPMLSDPDSDIKGWTAALDRLDALKPVGLVASRGNPTPEAAPAIAQTRQYLT
                     SLLQFLTAKRKQNAPEARVSGELAGEKIGDYCPRELSTLNALSVYRRIAADGSTVPGS
                     VQAPKAAPK"
     gene            complement(878..1258)
                     /gene="queF"
                     /locus_tag="PLB01_08295"
                     /pseudo
     CDS             complement(878..1258)
                     /gene="queF"
                     /locus_tag="PLB01_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
     gene            1329..2036
                     /gene="lepB"
                     /locus_tag="PLB01_08300"
     CDS             1329..2036
                     /gene="lepB"
                     /locus_tag="PLB01_08300"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQQ77343.1"
                     /translation="MAFTPEKRAALLKDIRHLALMAAVILTGRSVLADWYVVPTGSMK
                     PTILEGDRVFVWKSAYQMRVPFSRIRLFGTGTPLRGDVVVVRNPDGGSVPFVKRLVGL
                     PGDVVELRNETLYINGKAQPTDFLPEQKADDGETVLLGTEMLGARPHPIRVLPDRPAF
                     RTFGPITVPEGEVFLMGDNRDESRDARFFGTRPVTDLLGRAVGVMWSWNQEFLKGPRW
                     SRLARPFITSASGATIN"
     gene            2085..2504
                     /locus_tag="PLB01_08305"
     CDS             2085..2504
                     /locus_tag="PLB01_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQQ77344.1"
                     /translation="MTDRAALEGRLREKGLRVTQQRVAVLEYLCSTPTHPTAEEVGAA
                     VNRVQPTTSRASVYNVLNSLKESGLIEELVFDDAVARYDANLGPHHHFLCTSCGVVHD
                     VPFDALPALPKKRLPTGHTVESVDVTLRGLCPGCART"
     gene            2587..>3598
                     /locus_tag="PLB01_08310"
     CDS             2587..>3598
                     /locus_tag="PLB01_08310"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQQ77345.1"
                     /translation="MAVATAASAWRILATGVPSRVAAGLVRAAADDGEPAPEIVDVDA
                     FGELEWEGIRRGVRDVLLVWVDGTGDEGLEHLRVLQARQPGAPVLARVPKGRPDLVTR
                     MLRAGVHEVFSDAGRFREALDRARARVPSPDQGRPDSRSRPTGDEPDHGVEGLFPNLF
                     DWIYVVGVAEDGALVFQTVNPPLNAGIGFLNPDFAGRAVWKCLEGTSAAQLVPHLDRV
                     ISEGTPLQFEEEHLVGGRTRVFQTVLTPVRNKWGRIHRIAGISRDITALKEALADLRA
                     SEERLAHALEGTQQALWDWDLATGRLYRSPRWFEMLGLEPGSVDPTLEAGLDRVHPED
                     RRQ"
     assembly_gap    3599..3698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3771..5117
                     /locus_tag="PLB01_08315"
     CDS             3771..5117
                     /locus_tag="PLB01_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ77346.1"
                     /translation="MAESRILLVEDDAAVRHGLAAFLRASGLDVDEADTLRKALERFR
                     EVEHDIVVADYSLPDGTSLELLPEVKKVSEETPFIILTAHGSIDLAVRAIKEGAEQFL
                     TKPVESKALLVLVRRLLQQQRLRRKQEIVQREQPGSFDPFLGASTAIRQLEERARLML
                     EWDSPVLILGETGSGKGVLARWLHANGPRREEAFVNLNCAGLSRELLESELFGHERGA
                     FTGAVNPKQGLLEVGHRGIVFLDEIGDMDPAIQPKLLKALEEKTFRRLGDVRDRHVDI
                     RLLASTNLDLEEAVRARKFRDDLYYRVSTLTLKIPPLRERSEDIPLLARNILLAVCAR
                     MGKAEVVLGPEAESQLLRYPWPGNIRELANVLERAMILLKGDRLEALDVSLGSARPSA
                     PHDGELATMKEMERLHIERVLQHTGGNVAEAAAVLGMARRTLYDRLKALGLAPAAD"
     gene            5257..6216
                     /locus_tag="PLB01_08320"
     CDS             5257..6216
                     /locus_tag="PLB01_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77347.1"
                     /translation="MKTRALSRLLLATLVAAAGMATAADEAKPLHTRTIAKYPAPKLE
                     DVGGREYKFLIDPDKAKGTPEEAFKAIWTQVKAAAAKKGFTVTEKEKNPLKVELSTKE
                     YFDTPEQALWSKGFLVRITTKYKDGKPDETVSVTVKAIQEDALKTLATPLAVVDLKTK
                     TEAEGNVGPAPGGGLVEIVEKGSSFSVKPADLGGMTLGDFGKFMPELLKLGLPATTKL
                     VGTKAWSYRVRPGAVVLPGTEPCGVSMEGWATKEGGAPYLYDFSYGYGDLDFYAVAET
                     HTAGEQFLLKVVMGELGSLGMPDGAKWGGSKVRRLMNRPVPAN"
     gene            6250..7095
                     /locus_tag="PLB01_08325"
     CDS             6250..7095
                     /locus_tag="PLB01_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77348.1"
                     /translation="MRQTLKTLLGGLLLSALPLAAGDAPSLDQLYGVPSQPAYIKHYE
                     ADKTDKVILNPYLQVASKDFPAILDVKMAPYNWVIDVNGRVSLMPEAAHPLGRTYEKG
                     FFRPEDKSERKPGTRENFGHVSALGGAPGRISGEILYDKATNAWTLNNKSGRYTKHNS
                     DRTPEQLVNAANLIHEVVDPGSATWGGVYYLLDYAPEKIQEELLKSPKLAYDDAKKKS
                     RPHIVVMAAGAPAIKAEKAFEAAAAAPKPAETAAAKPAEKPAAPADAPKKAKKPKAAI
                     NDDPS"
     gene            7119..7886
                     /locus_tag="PLB01_08330"
     CDS             7119..7886
                     /locus_tag="PLB01_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HQQ77349.1"
                     /translation="MKKNLIKSGVVALALLLGTVLGASAVAQTLQPQVSVLKGEKNFW
                     TGYDPVNVDGSVNVVIEIAAGTTAKYMVELKTGMIELEQKNGGPRYVQYLGYPCNYGN
                     IPRSVLLKSKGGDGDAVDALVLGPSVPTGSVVRGRAIGMLSLVDTGEKDDKVVVVMEN
                     TPFFNVRSIDELDRKFPGVVSILQTWFTSYKGRDKDGKLFLSSPGFKGRAETIKFIGD
                     AVLDYENSVVTDADRRPLDEKGNPYLYRWPGAKNVGE"
     gene            8165..9532
                     /locus_tag="PLB01_08335"
     CDS             8165..9532
                     /locus_tag="PLB01_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQQ77350.1"
                     /translation="MKKSIVTPLLLALLAAPVMAQEPTDTEKRIRDLEKQVEALQKKD
                     AAKPAKAADAEPAKKSKGIELKTVTDKGQLSWETADGAYKFRLTGRVQFDGLFNSDSD
                     NRLSNGFTLRRVRLGYKATIAKDWISELSVDFAENAVDIKDAFIGYKGITDSTIQAGH
                     FKVPFGFDTLTSSANIWFVERSYSDAWTPDRRLGFGYYYGAERWQAAADIFMQTISVD
                     ATGVDQGWGWAVRASGAPLYKSKKEALHIGFAYNNRTPDAAAASSAAFAPIVYAIDFS
                     SRPEATKVSKAKFLNSGTMNEVDNVAQWAAELAGVWGPISWQGEYQESKVNRRSGNPN
                     LSDHKFSTWYGQAAWTFNGQRTYDPSEGLFGRVAPGKGGAYELLVRYSQMDLNDITTV
                     DPIKGGSAKNLTFGVNYYPNYNIRLMLNYTLVDNDEYAKPKTTYGGLTNDDFNELQFR
                     IQFAF"
     gene            9653..10246
                     /locus_tag="PLB01_08340"
     CDS             9653..10246
                     /locus_tag="PLB01_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77351.1"
                     /translation="MTHTSAMVIPRWEWRTFAPSLEDLRRKVNGAVFQAPREVRETYF
                     VCLKSSHNAKVRDGRMDLKWRKQVDADGLELWDPVLKSSFPVEAGVLPRLFEAWGLPL
                     PALPRPAYTLSELLDEVVEPNPALRAVRTVKRREGFTLDGTTCEFARIVADGIPLESF
                     CVEHEDPSLVLHVMRNLGLDSHQNINYPLGLKRALAL"
     gene            10273..10746
                     /locus_tag="PLB01_08345"
     CDS             10273..10746
                     /locus_tag="PLB01_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HQQ77352.1"
                     /translation="MAKEIERKFLVKTAAWKPQGPGVHFKQGYLNTQKERVVRVRIEG
                     SKAKLTIKGVTTGVTRSEFEYVIPVEDASILLDNLCEKPLIDKHRHAEVNGGKTWEID
                     VFHGDNEGLVVAEIELGSESETFVRPDWAGEEVSSDPRYFNSNLLKNPFKNWGKA"
     gene            10754..12316
                     /locus_tag="PLB01_08350"
     CDS             10754..12316
                     /locus_tag="PLB01_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQQ77353.1"
                     /translation="MTDAPAATRPPTLQELLDMDKMTLSAKPRSAQSATERWMRYLGF
                     PGGIALFLLVTYLPAGAGLSASAQAGAACFLLALVWWVTEPFPTYVTSLVLMFLLLVT
                     RTSAAKPIMDVLGMEVIWLNLLAFILAAMLVKTRLAKRMSLFLIVKFGRKASWALLAF
                     VIIQLALAPLIPATAARCVMTLPLMIVVANIYGSSEAHPNAFGKNLFLLNLAGISVLS
                     SISMTGSSANLIAVGFIQSMGGHRIYYMDWMRAGAPIAIVTMLVMWFLGRKVIFRIPA
                     AEQSPSVEGGLPLIEAQHAAMGPLSFAEKKAIAIFALVLLLWMTDIFHMRWFGVEISA
                     PFAALLGAVLVLFPRWGILQWQEADIPWHLLIFSAGAYAGGLALDETGAAEWGVRQLF
                     GGMDLKTVPFGAAFTIVMAVMMYSHLLTTSKTVRTIIMIPIIISMSKALGWDPVSLAL
                     PAALCIDWVVGLPISGKPNVILFSTNQYSVIDNFKYGMTTCTVGLVLLVLSAATWFHW
                     LGITPSFWAMPK"
     gene            12313..13053
                     /locus_tag="PLB01_08355"
     CDS             12313..13053
                     /locus_tag="PLB01_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77354.1"
                     /translation="MKPIRSFALALAVLASGLASGAEIREYSTRLDVDAAGAGRATGT
                     LVIAGNPSETLDVPLSHPAWANFRLGDASEGLRLEPPAEKAATVRVTLPPAAGGSRLS
                     FTYDVPEVFAKAEDASAGMKLTMPRDSRTLRYAFVNSQVTVIKDFRVLVVLPEGVRFQ
                     AIREQLPKQTRSEAEPRVRLGGEGGRQTALLRLANLRQGDDTSMQLEAVPGRRSLLWL
                     VAGVVLSVLYLVKFRDLVSTSDGPAQPR"
     gene            13063..13701
                     /locus_tag="PLB01_08360"
     CDS             13063..13701
                     /locus_tag="PLB01_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77355.1"
                     /translation="MSDPFARNTATSAEEASKIVPRAEFRVFGTGVIDVVKDRMWNGK
                     TVLFQARKMPAETYFLSRHTNEANVKVRDGLLDIKTKVGETPEGYEIFQPRGKFQFPV
                     RKADLETILSHLKVAMALTQDTYTIDEFIAMARRHPDMTAVSVEKMRYGFTIDGVICE
                     YAQVWFNGALLESACAESENYAGMKAVVEGLSIASLPNTNYLRAAKRVVGMD"
     gene            13730..14377
                     /locus_tag="PLB01_08365"
     CDS             13730..14377
                     /locus_tag="PLB01_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQQ77356.1"
                     /translation="MTLLFRRTRALEAQIDELLDAISEGTLVFQQGIADYLAKDAERF
                     EQRLKAIATFESKADALRREIETRLYRESLIPDHRGDVLGLLENIDNVIDEAKKTLHQ
                     FAVERPDVPADLTGDYLELSKAGVDAAQHLVSAVRAFFRDITAVPDHIHKVYFYEKEA
                     DRIADRLKRKIFSRADLDLARKIHLRYFCLHVETLSDRAEEVADRLAIYTIKRTL"
     gene            14393..>14690
                     /locus_tag="PLB01_08370"
     CDS             14393..>14690
                     /locus_tag="PLB01_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQQ77357.1"
                     /translation="MKWLFLSSGLFLGWTLGANDAANLFGTAVGTRMIKFRTAALVAS
                     VFVILGAVSGGAGTTRTLGRLGTIDAPAGAFGVALAAGRAVTLLRTRRGLPVWMT"
BASE COUNT         2444 a         5266 c         4743 g         2137 t
ORIGIN      
        1 gcccccgccg cggccgtgcc ggccgtcgcg cagcccttca aggacgttgg cctctggtac
       61 ggcgtcttcg gaacgacgat ctgctcgtgg ttcgacgccg gcgacggcgt gttcctcgtc
      121 gacaccggct cgaccgccgc cgacgcgaag gcgctccgcg ccgagatcgc gaagacgacg
      181 aagaacaagc ccgtgaaatg gatcgcgatg acccacctcc acacggactc gaacgaaggc
      241 ctcgcgacgt tcttcccggc cgacgtcacg atcttcgtga acgcgcgctc ggcggcgact
      301 ctcgcggact tcgccgggcg acagaagagc ccgaccccga acgtggtcgg cgtcgccgac
      361 cggatcgtcc tcggcctcgg caagctccgc ctcgagatcg gcgtgcctcc cggcaacgcg
      421 cacaccggct cggacctcta cgtcttcgac gctgtgacgc ggaccgcgtt cgtcggcgac
      481 ctcgtgacgc ccgatcgctg cccgatgctc tcggacccgg attccgacat caagggctgg
      541 accgcggcgc tggaccggct cgacgcgctg aagcccgtcg gcctcgtcgc gtcgcgcggg
      601 aaccccacgc cggaagcggc gcccgcgatc gcgcagacgc gccagtacct cacgagcctc
      661 ctccagttcc tcacggccaa gaggaagcag aacgcacccg aggcgcgcgt ctccggcgag
      721 ctggccggcg agaagatcgg ggactactgc ccgcgcgagc tgagcacgct gaacgcgctc
      781 agcgtctacc ggcggatcgc ggccgacgga tcgaccgtgc cgggctcggt ccaggcgccg
      841 aaggccgctc cgaagtgatc cccgcaccgc cggggccgcg cggcgcgcgg gcctcgacgg
      901 tcgtctggat gccgccgcgg ggcgtgaaca cgccccgcac ggccatccgg cgcggatgga
      961 gcgccgccac gaggtcctcg aggacgcggt tcacgacgtt ctcgttgaag atcccgagcg
     1021 agcggtacgc gttcacgtac tccttgagcg acttcagctc gacgcagagc cggtcgggca
     1081 cgtaggtgat cgtgagcgtc gcgaagtccg gaagccccgt cttcgggcag atcgacgtga
     1141 actccgggat cgtgatcacg acctcgtagt cggccgcgta ctgcgacggc caggtctcga
     1201 ggccgggaag cgcggccttc acgcccgagg cggcgtggcg ttcggtgtag ccggacatgc
     1261 ggcggaggat agcgcggacc cccgaagaag gaaggcctgg cacgccctgt gcgagaatcc
     1321 gcgtccccat ggcattcact ccggaaaagc gcgccgccct cctcaaggac atccgccacc
     1381 tcgccctgat ggccgccgtc atcctgacgg gacggtccgt tctggccgac tggtacgtcg
     1441 ttccgacggg gtccatgaag cccacgatcc tcgaagggga ccgcgtcttc gtgtggaaat
     1501 cggcctacca gatgcgcgtg ccgttctcgc gcattcgcct tttcggcacc ggcacgccgc
     1561 tgcgcggcga cgtcgtcgtc gtgcgcaacc ccgacggcgg gagcgtcccg ttcgtcaagc
     1621 gcctcgtcgg cctgcccggc gacgtcgtgg agctgcgcaa cgagacgctc tacatcaacg
     1681 gcaaggccca gccgaccgac ttcctgcccg agcagaaggc ggacgacggc gagaccgtcc
     1741 tcctcggcac cgagatgctc ggggcgcgcc cgcacccgat ccgcgtcctc ccggaccgcc
     1801 cggcgttccg cacgttcggt ccgatcaccg tgcccgaggg cgaagtgttc ctcatgggcg
     1861 acaaccgcga cgagagccgt gacgcgcggt tcttcggcac gcgcccggtc acggacctcc
     1921 tcggccgggc cgtcggcgtg atgtggagct ggaaccagga attcctcaag gggccgcgct
     1981 ggtcccgcct cgcgcgcccg ttcatcacga gcgcgagcgg cgcgacgatc aactgaccct
     2041 tcccttctct tgatttagtt gatctttaga cttaatctaa gctggtgacg gatcgtgccg
     2101 cacttgaggg gcgcctcaga gagaagggac tgcgcgtgac gcagcagcgc gtcgctgtgc
     2161 tcgagtacct ctgttcgacg ccgacgcacc cgacggccga ggaggtcggc gccgccgtga
     2221 accgcgtcca gcccacgacc tcgcgcgcct ccgtctacaa cgtcctcaac tccctcaagg
     2281 agagcggcct catcgaggag ctcgtcttcg acgacgccgt ggcgcgctac gacgcgaacc
     2341 tcggcccgca ccaccacttc ctctgcacgt cctgcggcgt ggtccacgac gtcccgttcg
     2401 acgcgctccc ggcgctcccg aagaagcgcc tcccgaccgg ccacacggtc gagagcgtcg
     2461 acgtgacgct gcgcggcctc tgccccggct gcgcgcggac ctgacccgcg ggactgattc
     2521 aaccgcaccg tttccgtgac ccggccggag ggccagactg gcggcggaag gagtcggcac
     2581 gccgccatgg ccgtagcgac cgcagcgtcc gcctggcgga tcctcgcgac gggcgttccg
     2641 tcccgcgtcg ccgcaggact cgtccgcgcc gcggcggacg acggggagcc ggccccggag
     2701 atcgtcgacg tcgacgcctt cggtgagctc gagtgggagg gaatccgccg tggggtgcgg
     2761 gacgtcctcc tcgtctgggt ggacgggacc ggcgacgagg gcctcgagca cctccgcgtg
     2821 ctccaggcaa ggcaacccgg ggcgccggtc cttgcacgcg ttccgaaggg ccggccggac
     2881 ctcgtcaccc ggatgctgcg cgccggagtc cacgaggtct tcagcgatgc cgggcgtttc
     2941 cgagaggcgc tcgaccgcgc ccgcgcccgc gtcccgtcgc cggaccaggg ccgccccgac
     3001 tcgcgctccc ggccgacagg ggacgagccg gaccacggcg tcgagggtct ctttccgaac
     3061 ctcttcgact ggatctacgt ggtcggcgtg gccgaggacg gcgccctggt tttccagacc
     3121 gtgaaccccc cgctcaacgc cggaatcggc ttcctgaatc ccgatttcgc ggggcgtgcg
     3181 gtgtggaagt gtctcgaggg gacgagcgcg gcgcagctcg tcccgcacct cgaccgcgtc
     3241 atctccgagg ggacgccgct gcagttcgag gaggaacatc tcgtcggcgg gcgcacgcgc
     3301 gtctttcaga ccgtcctgac ccccgtgcgc aacaagtggg ggcggatcca ccggatcgcg
     3361 gggatttcgc gcgacatcac ggcgctgaaa gaggcgctcg cggatcttcg cgcgagcgag
     3421 gaacgcctcg cccacgcgct ggagggaacg cagcaggccc tctgggactg ggacctcgcg
     3481 acgggccgcc tctaccgcag cccgcgctgg ttcgagatgc tcggcctcga gcccggctcc
     3541 gtcgacccga cgctcgaagc cggcctcgac cgggtccatc ccgaggaccg ccgtcaggnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gagggcgggg cccgcgtcgt
     3721 cgtccggctc ccggcgccgc gccagaggat ggagctcccg gagagcgcgc gtggctgagt
     3781 cgcggatcct cctcgtggaa gacgacgccg cggtgcggca cgggctcgcg gccttcctgc
     3841 gcgcgagcgg cctcgacgtg gacgaggccg acaccctccg caaggcgctc gagcgctttc
     3901 gcgaggtcga gcacgacatc gtcgtggcgg actacagcct cccggacggg acgtcgctcg
     3961 aactcctccc ggaggtcaaa aaggtcagcg aggagacgcc gttcatcatc ctgacggcgc
     4021 acgggtcgat cgatctcgcc gtccgggcga tcaaggaggg cgccgagcag ttcctcacga
     4081 agccggtgga gtccaaggcg ctcctcgtcc tcgtgcggcg tctcctgcag cagcagcgcc
     4141 tccgtcgcaa gcaggagatc gtccagcgcg aacagccggg ctcgttcgac cccttcctcg
     4201 gcgcgagcac ggcgatccgg cagctggagg aacgcgcccg cctcatgctc gagtgggaca
     4261 gccccgtcct catcctcggc gagacgggct cgggcaaggg cgtcctcgcc cgctggctcc
     4321 acgcgaacgg gccgcgccgg gaagaggcgt tcgtcaacct gaactgcgcg ggcctttcgc
     4381 gcgagctcct cgaatccgag ctcttcgggc acgagcgcgg ggcattcacg ggcgccgtga
     4441 acccgaagca ggggctcctc gaagtcggcc accgcgggat cgtcttcctc gacgagatcg
     4501 gcgacatgga ccccgccatc cagccgaagc tcctgaaggc gctcgaggag aagacgttcc
     4561 gaaggctcgg cgacgtgcgg gaccggcacg tcgacatccg gctcctcgcc tcgacgaacc
     4621 tcgacctcga ggaggccgtg cgcgcacgga agttccgcga cgacctctac taccgcgtca
     4681 gcacgctgac gctgaagatc cctcccctgc gcgagcggtc ggaggacatc ccgctcctgg
     4741 cgcggaacat cctcctcgcc gtctgcgcgc gcatgggcaa ggcggaggtc gtcctcgggc
     4801 cggaggccga gtcgcagctc ctgcgttacc cgtggcccgg gaacatccgc gaactcgcga
     4861 acgtgctcga gcgggcgatg atcctgctca aaggcgaccg gctggaggcc ctcgacgtca
     4921 gcctcggctc cgcgcggccg tccgccccgc acgacggcga gctcgcgacg atgaaggaga
     4981 tggagcggct ccacatcgag cgcgtgctcc agcacacggg cggcaacgtc gcggaggccg
     5041 ccgccgtcct cggcatggcc cgccggacgc tttacgaccg actcaaggcg ctggggctcg
     5101 ctcccgccgc ggactgagcg gggggccgca cgccgcgtgc gggtttccgc actcctcgcg
     5161 cgcgggcccg ccacgtatcc ggctttcctg caaggactaa gcccgctcgc ccgtgtggct
     5221 cgcgccttgc acgacggcgg gcgcaggagg attctcatga agacccgtgc cctctcacgg
     5281 ctgctgctcg cgacgctcgt cgcggccgcc ggcatggcga cggccgccga cgaggccaag
     5341 ccgctccaca cccgcacgat cgccaagtac cccgcgccca agctcgaaga cgtcggcgga
     5401 cgcgagtaca agttcctcat cgaccccgac aaggccaagg ggacgccgga agaggcgttc
     5461 aaggccatct ggacgcaggt gaaggccgcc gccgccaaga aggggttcac ggtcaccgag
     5521 aaggagaaga acccgctcaa agtcgagctc tccaccaagg agtacttcga cacgcccgag
     5581 caggcgctct ggagcaaggg cttcctcgtg cgcatcacga cgaagtacaa ggacggaaag
     5641 cccgacgaga ccgtctccgt cacggtcaag gcgatccagg aagacgcgct caagaccctc
     5701 gcgacgccgc tcgcggtcgt cgacctcaag acgaagaccg aggccgaggg caacgtcggc
     5761 cccgcgcccg gcggcggcct ggtcgagatc gtcgagaagg gctcctcgtt ctccgtcaag
     5821 cccgccgatc tcggcggaat gacgctcgga gacttcggga agttcatgcc cgagctcctg
     5881 aagctcggcc tcccggccac gacgaagctc gtcggcacga aggcctggtc gtaccgcgtc
     5941 cgcccgggcg ccgtcgtcct gcccggcacc gagccgtgcg gcgtctcgat ggagggctgg
     6001 gccacgaagg agggcggcgc tccgtacctc tacgactttt cctacggcta cggagatctc
     6061 gacttctacg ccgtcgccga gacgcacacc gccggcgagc agttcctcct caaggtcgtc
     6121 atgggcgagc tgggctctct cggcatgccc gacggcgcga agtggggcgg ctcgaaggtc
     6181 cgcaggctca tgaaccgccc ggtcccggcg aactagccgg gcctcaacaa cgcaaggagt
     6241 cacatcgcga tgagacagac gctcaagacc ctcctcggag gcctgctcct gtcggcgctg
     6301 ccgctcgccg ccggggacgc gccctcgctc gaccagcttt acggggtccc ctcgcagccc
     6361 gcctacatca agcactacga ggcggacaag acggacaagg tcatcctgaa cccgtacctg
     6421 caggtcgcct ccaaggactt cccggccatc ctcgacgtga agatggcgcc gtacaactgg
     6481 gtcatcgacg tgaacggacg cgtgtccctc atgcccgagg ccgcccaccc gctgggccgc
     6541 acgtacgaga agggcttctt ccgccccgag gacaagtccg agcgcaagcc ggggacgcgc
     6601 gagaacttcg gccacgtctc ggctctcggc ggggcgcccg gccggatcag cggcgagatc
     6661 ctctacgaca aggccacgaa cgcctggacg ctcaacaaca agtccggccg ctacacgaag
     6721 cacaactccg accgcacgcc cgagcagctc gtgaacgccg cgaacctcat ccacgaggtc
     6781 gtggacccgg gcagcgcgac ctggggcggc gtctactacc tcctcgacta cgctcccgag
     6841 aagatccagg aagagctcct caagagcccg aagctcgcgt acgacgacgc gaagaagaag
     6901 agccgcccgc acatcgtcgt gatggccgcg ggggcccccg cgatcaaggc ggagaaggcc
     6961 ttcgaggccg cggccgccgc gccgaagccg gccgagaccg ccgccgccaa gcccgccgag
     7021 aaacccgccg cgcccgccga cgcgcccaag aaggcgaaga agcccaaggc cgcgatcaac
     7081 gacgacccgt cgtgaacgaa acgaggagct gacacaccat gaagaagaac ctcatcaagt
     7141 ccggcgtcgt cgccctcgcg ctgctgctcg ggacggtcct gggcgcgtcg gccgtggcgc
     7201 agactctcca gccgcaggtc tcggtcctca agggcgagaa gaacttctgg accggctacg
     7261 acccggtcaa cgtcgacggc tcggtcaacg tcgtcatcga gatcgccgcc ggcacgaccg
     7321 cgaagtacat ggtcgagctg aagaccggga tgatcgagct cgagcagaag aacggcgggc
     7381 cgcggtacgt gcagtacctc ggctacccgt gcaactacgg caacatcccg cgctcggtgc
     7441 tcctgaagtc caagggcgga gacggcgacg ccgtggacgc tctcgtcctc ggtccgtccg
     7501 tcccgacggg ctccgtcgtg cgcggccgag cgatcggcat gctgagcctc gtggacacgg
     7561 gcgagaagga cgacaaggtc gtcgtcgtca tggagaacac gccgttcttc aacgtgcgca
     7621 gcatcgacga gctggaccgc aagttccccg gcgtggtctc gatccttcag acctggttca
     7681 cgtcgtacaa gggccgcgac aaggacggca agctgttcct ctcctcgccg ggcttcaagg
     7741 gccgcgccga aacgatcaag ttcatcggcg acgccgtcct cgactacgag aattccgtcg
     7801 tgacggacgc cgaccggcgc ccgctcgacg agaagggcaa tccctatctc taccgctggc
     7861 cgggtgcgaa gaacgtcggc gagtaacccg gatcccgact cctccccctc ctccgaaagg
     7921 ggaagcggac cacctcctgt ccgtttcccc tctttttttt cgccaggccc gcgcggacgc
     7981 ccgcactgcc cgtgcgggtt cccgcacgcc gggcgcttcg tccgcaggcc cggaacccga
     8041 tccaacacgc tcattccaca cgacttgacg taatcgcacg agatggctcg ggccttgcga
     8101 agcaggggca tacgttcatg tgcctcgccc gcgaggcaag gccccaaaac gagtaggagt
     8161 gaccatgaag aagtcgatcg tgaccccgct gctcctcgcc ctgctggccg ccccggtgat
     8221 ggcgcaggag cccacggaca ccgaaaaacg aatccgcgat ctcgagaaac aggtcgaggc
     8281 gctccagaag aaggacgccg ccaagcccgc gaaggccgcg gacgccgagc cggccaagaa
     8341 gtccaagggc atcgagctca agaccgtgac ggacaagggc cagctctcgt gggaaaccgc
     8401 ggacggcgcc tacaagttcc gtctcaccgg ccgcgtccag ttcgacggcc tcttcaacag
     8461 tgactccgac aaccgccttt cgaacggctt caccctccgc cgcgtccgcc tcggctacaa
     8521 agcgacgatc gcgaaggact ggatctccga gctctccgtc gacttcgccg agaacgccgt
     8581 cgacatcaag gacgcgttca tcgggtacaa gggcatcacg gacagcacga tccaggccgg
     8641 ccacttcaag gtgccgttcg gcttcgacac gctgacgtca tccgccaaca tctggttcgt
     8701 cgagcgctcc tacagcgacg cctggacgcc ggaccgccgc ctcggcttcg gctactacta
     8761 cggcgccgaa cgctggcagg ccgccgccga catcttcatg cagacgatct ccgtcgacgc
     8821 cacgggcgtc gaccagggct ggggctgggc cgtccgcgcg tccggcgcgc cgctctacaa
     8881 gtccaagaaa gaggcactcc acatcggctt tgcgtacaac aaccgcacgc cggacgccgc
     8941 cgcggccagc tcggccgcct tcgcgccgat cgtgtacgcg atcgacttct cctcgcggcc
     9001 cgaggccacg aaggtcagca aggccaagtt cctcaacagc gggacgatga acgaggtcga
     9061 caacgtggcc cagtgggccg ccgagctcgc cggcgtctgg ggccccatct cctggcaggg
     9121 cgagtaccag gagtccaagg tcaaccgccg ctcgggcaac ccgaacctgt cggaccacaa
     9181 gttctcgacg tggtacggcc aggccgcgtg gaccttcaac ggccagcgca cgtacgaccc
     9241 gagcgagggc ctcttcggcc gcgtcgcgcc gggcaagggc ggagcgtacg agctcctcgt
     9301 gcggtacagc cagatggacc tcaacgacat cacgacggtc gacccgatca agggcggctc
     9361 cgccaagaac ctcacgttcg gcgtgaacta ctacccgaac tacaacatcc gcctcatgtt
     9421 gaactacacg ctcgtcgaca acgacgagta cgccaagccg aagacgacct acggcggcct
     9481 cacgaacgac gacttcaacg aactgcagtt ccgcatccag ttcgcgttct agcgaaccgg
     9541 gactgacgcc gggagagccc gcctccgcgg gttctcccga tccctgatcc cggcgtcccg
     9601 ccccggccga ccggccggga cacgctcgac cccaacgcag ctggagccga cgatgactca
     9661 cacgtccgcc atggtgattc cacgctggga gtggcgcacg ttcgccccgt ccctggagga
     9721 ccttcgccgc aaggtgaacg gcgccgtctt ccaggctccg cgggaggtcc gggagaccta
     9781 cttcgtctgc ctgaagagct cgcacaacgc gaaggtccgc gacgggcgca tggacctcaa
     9841 gtggaggaag caggtggacg cggacggcct cgagctctgg gacccggtcc tcaagtcctc
     9901 gttccccgtc gaggccggcg tcctgccgcg gctcttcgag gcctggggcc tccccctgcc
     9961 ggccctcccg cggccggcct acacgctttc cgagctcctc gacgaggtcg tcgagccgaa
    10021 ccccgcgctc cgcgcggtcc gcaccgtcaa gcggcgggag ggcttcacgc tcgacgggac
    10081 gacgtgcgag ttcgcgcgca tcgtggctga cggcatcccg ctcgagagtt tctgcgtcga
    10141 gcacgaagac ccctccctcg tcctccacgt catgcggaac ctcggactcg acagtcacca
    10201 gaacatcaac taccccctgg ggctcaagcg agccctggcg ctctaaacgg gctcgcgggc
    10261 cccggagaca acatggccaa ggaaatcgaa cgcaagttcc tcgtgaagac cgccgcctgg
    10321 aagccccagg ggcccggcgt tcacttcaag cagggttacc tgaacaccca gaaggagcgg
    10381 gtcgtccggg tgaggatcga gggctccaag gcgaagctca cgatcaaggg tgtcacgacg
    10441 ggcgtgacgc gttcagaatt cgagtacgtg atccccgtcg aagacgcctc catcctcctc
    10501 gacaacctct gcgagaagcc cctcatcgac aagcaccgcc acgcggaggt caacggcggc
    10561 aagacgtggg agatcgacgt cttccacggg gacaacgagg ggctcgtcgt cgcggagatc
    10621 gagctcggca gcgagagcga gacgttcgtc cgcccggact gggcgggcga ggaagtctcg
    10681 agcgatccgc gttacttcaa ctccaacctc ctgaagaacc cgttcaagaa ctggggcaag
    10741 gcctgacccg gcgatgaccg acgcacccgc ggcgacccgc cctccgacgc tccaggagct
    10801 cctggacatg gacaagatga cgctctcggc gaagccgcgc tccgcccaga gcgccacgga
    10861 gcgctggatg cggtacctcg ggttccccgg ggggatcgcc ctgttcctgc tcgttaccta
    10921 cctgcccgcg ggggcggggt tgagcgcgtc cgcgcaggcg ggggcggcct gcttcctcct
    10981 cgcgctcgtg tggtgggtga ccgagccgtt cccgacctac gtgacgtcgc tcgtcctcat
    11041 gttcttgctt ctcgtgacgc gcacgtcggc ggccaagccg atcatggacg tcctcggcat
    11101 ggaggtcatc tggctgaacc tcctggcctt catcctggcc gcgatgctcg tgaagacgcg
    11161 cctcgccaag cgcatgtcgc tcttcctcat cgtgaagttc gggcgcaagg cgtcctgggc
    11221 gctcctcgcc ttcgtgatca tccagctcgc gctcgcgccg ctcatccccg cgaccgccgc
    11281 gcgctgcgtc atgacgctcc cgctcatgat cgtcgtcgcg aacatctacg ggtcgagcga
    11341 ggcccacccg aacgcgttcg ggaagaacct cttcctcctg aacctcgcgg gcatctccgt
    11401 gctgtcgtcg atcagcatga cgggctcgag cgcgaacctc atcgccgtcg gcttcatcca
    11461 gagcatgggc ggacaccgca tctactacat ggactggatg cgggccggcg ccccgatcgc
    11521 gatcgtgacg atgctcgtca tgtggttcct cggccgcaag gtgatcttcc ggatcccggc
    11581 cgccgagcag tccccgagcg tcgagggagg cctcccgctc atcgaggccc agcacgcggc
    11641 gatggggccc ctgagcttcg cggagaagaa ggccatcgcg atcttcgcgc tcgtgctctt
    11701 actttggatg acggacatct tccacatgcg ctggttcggc gtggagatca gtgcgccgtt
    11761 cgcggcgctc ctcggcgccg tcctcgtcct cttcccgcgc tggggcatcc tgcagtggca
    11821 ggaggccgac atcccgtggc acctcctgat cttcagcgcg ggcgcctacg cgggcggcct
    11881 cgccctggac gagacgggcg cggccgaatg gggtgtgcgg cagctcttcg ggggaatgga
    11941 cctgaagacg gtcccgttcg gcgcggcctt cacgatcgtc atggccgtca tgatgtacag
    12001 ccacctgctc acgacgtcga agacggtgcg cacgatcatc atgatcccga tcatcatctc
    12061 gatgtcgaag gccctgggct gggaccccgt ctcgctcgcg cttccggccg ccctgtgcat
    12121 cgactgggtc gtgggtcttc cgatcagcgg gaagccgaac gtgatcctct tctccacgaa
    12181 ccagtattcc gtcatcgaca acttcaagta cgggatgacg acctgcaccg tggggctcgt
    12241 cctcctcgtc ctctccgcgg cgacgtggtt ccactggctc gggatcacgc cctccttctg
    12301 ggcgatgccg aagtgaagcc gatccggagc ttcgccctcg ccctcgccgt cctcgcgtcc
    12361 ggcctcgcgt cgggagcgga gatccgcgag tactcgacgc ggttggacgt ggacgccgcc
    12421 ggcgccggcc gcgccaccgg cacgctcgtc atcgcgggga acccgtccga gacgctcgac
    12481 gtccccctga gccaccccgc ctgggcgaac ttccggctcg gggacgcgtc cgaggggctc
    12541 cggctggagc cgccggcgga gaaggccgcc acggtgcgcg tgacgctgcc ccccgccgcc
    12601 ggcgggagcc gtctctcctt cacgtacgac gtcccggagg tgttcgcgaa ggccgaggac
    12661 gcgtccgcgg ggatgaagct cacgatgccg cgcgacagcc ggacgctgcg ctacgcgttc
    12721 gtgaactcgc aggtgaccgt catcaaggac ttccgcgtcc tcgtcgtcct ccccgagggc
    12781 gtccgcttcc aggccatccg cgagcagctc ccgaagcaga cgaggtccga agcggagccc
    12841 cgtgtccgcc tcggaggcga aggcggccgc cagaccgcgc tcctgcgcct cgcgaatctc
    12901 cggcagggcg acgacacctc catgcagctc gaggccgtcc cgggccgccg gtcgctcctg
    12961 tggctcgtcg cgggcgtcgt cctctccgtc ctgtatctcg tcaagttccg ggacctcgtc
    13021 tccacgagcg acgggcccgc gcagcctcga taaggagcag tcatgtcgga tccgtttgcc
    13081 aggaacaccg ctacgtcggc ggaagaagcc tcgaagatcg tgccccgcgc cgagttccgc
    13141 gtgttcggca cgggcgtgat cgacgtcgtg aaggacagga tgtggaacgg gaagaccgtg
    13201 ctcttccagg cccggaagat gccggccgag acgtacttcc tgagccgcca cacgaacgag
    13261 gcgaacgtga aggtccgcga cggcctcctc gacatcaaga cgaaggtcgg cgagacgccc
    13321 gagggctacg agatcttcca gccgcgcggc aagttccagt tccccgtccg gaaggcggac
    13381 ctcgagacga tcctgtcgca cctcaaggtc gcgatggcgc tgacccagga cacctacacg
    13441 atcgacgagt tcatcgcgat ggcccgcagg catcccgaca tgacggccgt gtccgtcgag
    13501 aagatgcgtt acggcttcac gatcgacggg gtcatctgcg agtacgccca ggtgtggttc
    13561 aacggagccc tgctggaatc ggcctgcgcc gagagcgaga actacgccgg gatgaaggcc
    13621 gtcgtcgaag ggctttcgat cgcgtccctt ccgaacacga actacctcag ggcggccaag
    13681 cgcgtcgtgg ggatggacta gtccccggcg cgcccccggg ggaacacgca tgacactcct
    13741 cttccgccgc acccgcgccc tcgaagcgca gatcgacgag ctgctcgacg cgatcagcga
    13801 ggggacgctc gtcttccagc agggcatcgc ggactacctc gcgaaggacg ccgagcggtt
    13861 cgagcagcgc ctgaaggcga tcgccacgtt cgagagcaag gccgacgccc tgcgccgcga
    13921 gatcgagacg cgcctctacc gcgagtcgct catcccggat caccgcggcg acgtgctcgg
    13981 cctcctcgag aacatcgaca acgtgatcga cgaggcgaag aagacgctcc accagttcgc
    14041 cgtcgagcgg cccgacgtgc ccgcggacct cacgggcgac tacctcgagc tctcgaaggc
    14101 gggcgtcgac gccgcccagc acctcgtctc ggccgtgcgc gcgttcttcc gcgacatcac
    14161 ggcggtgccc gaccacatcc acaaggtcta cttctacgag aaggaggccg accgcatcgc
    14221 ggaccgcctg aagcgcaaga tcttcagccg cgccgacctc gacctcgccc gcaagatcca
    14281 cctgcgctac ttctgcctgc acgtcgagac gctgtcggac cgggccgagg aagtggccga
    14341 ccggctcgcg atctacacga tcaagcggac gctctaggcg gacggcccgg ccgtgaagtg
    14401 gctcttcctc tcgagcgggc tgttcctcgg ctggacgctc ggcgccaacg acgccgcgaa
    14461 tctcttcggc accgccgtcg ggacgcggat gatcaagttc cggacggccg cgctcgtcgc
    14521 gagcgtgttc gtcatcctcg gcgccgtctc gggcggcgcg ggcacgaccc gcacgctcgg
    14581 ccgcctcggc acgatcgacg cgccggcggg cgcgttcggc gtcgcgctcg ccgccgggcg
    14641 cgccgtcacc ctcctccgga cgcggagggg cctccccgtg tggatgacgc
//
LOCUS       DAOMFY010000079        14511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1272_length_14511_cov_8.457941, whole genome shotgun sequence.
ACCESSION   DAOMFY010000079 DAOMFY010000000
VERSION     DAOMFY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14511
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_14511_cov_8.457941"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLB01_08375"
     CDS             complement(<1..384)
                     /locus_tag="PLB01_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77358.1"
                     /translation="MARRFALALVAILGLLLVAFLVMRSVRNPKAGAPPVPAPRPTAV
                     VPPTPIPARNVTLFFESTEGDGVLHPEARDVPVAADDVAFLRSVASGVVEGPRSADLM
                     RPFPEGWTLRGAYLLRDGVAVLDVAP"
     gene            complement(390..1916)
                     /locus_tag="PLB01_08380"
     CDS             complement(390..1916)
                     /locus_tag="PLB01_08380"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQQ77359.1"
                     /translation="MTRATARLLILLAAAALSSLFGSRAAHGEDASLARDGKPAAVIR
                     AEERDGRTYLLVNDAVAALDGTITWDERTRSYEMKVKGRTAVFGAESPIAVVDTKVVN
                     LASPVRASSLGAWAEPEFFVRVLGPLSGQALAWEKATRTLSARKSEFVEVGVEPSVAE
                     LGDVTKIVLRFSQSPSYAVETSPEQVVLHFPGARLTGPPPERVLDSPRVARLLVRGGE
                     VTVVFREKGLAANVYALGTPPRLVVDVTRRAGSGPSAAPAAPVPAPAAAKTAKTIVID
                     PGHGGTEEGAKGPGGSFEKDATLALGKTVAETLQRRGYRVVLTRTTDATVGLEDRSTA
                     ANAAKADVFLSLHCNASRAASAHGTEVYYLSLDASDRAAAALAESENRAAPGAASAEK
                     NAAVRDLDLILWDLAQNQHLGASARLAEIVQADFNRLLGITTRGVKQAPFRVLIGVNA
                     PAVLVEVSFITNPDEERKINSEEFRRQVADTLAGSLETYFRSADGAAPVPYAPKNGKS
                     "
     gene            1982..2339
                     /gene="ssrA"
                     /locus_tag="PLB01_08385"
     tmRNA           1982..2339
                     /gene="ssrA"
                     /locus_tag="PLB01_08385"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2415..3242)
                     /locus_tag="PLB01_08390"
     CDS             complement(2415..3242)
                     /locus_tag="PLB01_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77360.1"
                     /translation="MLREWIRGPVLDSCLERHAGRNADLNFILSIYLNRVEPDSSGLY
                     PDASGGITQILRWPDNEWRDFRGRFYREVTGFFSRRFWLIPPRGYTGLDWPEGRPTHR
                     PNVACGLTLWIRDRPEHARIIVNCIYPVPGQAMRSGVSPGNHSGELNAVDASYEDVQP
                     VPGEVQIHMTALHEVGHLLGLSHVARDPANCPVDNAVACYGTTAWQRGDLMGWGCRVE
                     YWHSWPWRNRIRYHTGIGGWRAVMTQPDPQPLGVNQGMDAGIRGPATGVPGIRDGGI"
     gene            3424..4965
                     /locus_tag="PLB01_08395"
     CDS             3424..4965
                     /locus_tag="PLB01_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ77361.1"
                     /translation="MRSTVLALCLGAALVSAALPAHAVLPPGVDDASIAAAAAATFPE
                     FLDLLALPNDSAKPADIQKNAAWLEAAFARRGFATRQIENNGRPLVFNEYGKARPAQK
                     TVLFYIHFDAQPVVPSQWAQKDPWLPVVKRRGADGTWAEVDRAELLKPDFDRDLRVFA
                     RSSSDDKGPIVMFLAVFDLLRAKKIDPAFHVKVLLDSEEEAGSPGIAAAVASNAALLS
                     ADAVVLLDGPVHASDRPTLAFGNRGVVGVTLTVYGPRAPLHSGHFGNWVPNPALRLAK
                     LLASMKDEDGRVTVAGYYGRTNLTDADRRVLAEVPDDEAALKKRTGIAQPEKVGATYQ
                     ESLQYPSLNVRGLASASVGEKAANVLPRDAVAELDLRTTPESDPAFLLGLLKKHVEAQ
                     GWHLVEKDPTDDERARWPKLARFDAHAPEEAARQAFEAPVGRWAANAVSGAWPSAPLV
                     RIRMMGGTLPTHAIVGPLKAPFVQVPVVNPDNNQHAYDENLRMGHYLSGMRTMAALLT
                     APYPE"
     gene            4981..5556
                     /locus_tag="PLB01_08400"
     CDS             4981..5556
                     /locus_tag="PLB01_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HQQ77362.1"
                     /translation="MRNHRRLPLMLAFCLAAPAFAQGRPDPAALLAAQKDALKAFAAM
                     DGVWRGPAWTLLPSGEKHTITQTERIGPFLDGSVKVIEGKGYEADGRVSFNAFGTISF
                     SPAKNAYTLHSYAMGSVGDFVIKPTPDGYTWEIPAGPMTIRYTAVIKDGTLKEVGDRL
                     EPGKDPVRFFEMTLTRIGDTTWPAGGAVPPK"
     gene            5565..6785
                     /locus_tag="PLB01_08405"
     CDS             5565..6785
                     /locus_tag="PLB01_08405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HQQ77363.1"
                     /translation="MNDLARRDFLRFLAASPLLAGLGGLDAFADGGEPLADSAGKALD
                     VFELEAIAKKNLPPAHWGYMATGVDGDATLRANSEGYANLALRVRRLAGAHKPDTSVS
                     LFGTAWETPIVLCPVSSQHAFHEDAEIATARAARSRRTLQILSTLSSTGVEDVAAARG
                     GPVWYQLYPTDQWSVAQGLMKRAEAAGCPALVLTVDLQGGSNRETVVRGRRLDTRDCS
                     SCHVADPFDVAGALPRRAMFKGLDVSKVTWIFPPDATWDYVAKIRAAWPRKLLLKGIV
                     TREDAELCLANGVDGIVVSNHGGRAEESLRPSIDSLVEVAAAVKGRVPVILDGGIRRG
                     TDIFKALALGATAVGIGRPQVWGLAAFGQAGVEAALDILRRELEMVMRQAGTTSLAKI
                     DRSYVVDRRRAPAG"
     gene            complement(6782..7210)
                     /locus_tag="PLB01_08410"
     CDS             complement(6782..7210)
                     /locus_tag="PLB01_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HQQ77364.1"
                     /translation="MPYRLVYSSEAAPDLTPAELEGMLAESRIRNKAHGISGVLVFVD
                     GAFLQVLEGEKNDVVDLMSRIDRDPRHRGVKVFYEQDVDERAFDSWSMAYLSPSAAEV
                     AKWAELEGATTIGDVLASVEAHPGRLPGMVVNILKALAKS"
     gene            complement(7308..7595)
                     /locus_tag="PLB01_08415"
     CDS             complement(7308..7595)
                     /locus_tag="PLB01_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQQ77365.1"
                     /translation="MKLYVGNLSYSVNDSTLKSLFEPFGNVESARVISDRDTGSSKGF
                     GFVEMSDEDAKKAMGALNGREQDGRALKVNEAKPQENRGGGGGFGGGRNRY"
     gene            complement(7803..8576)
                     /locus_tag="PLB01_08420"
     CDS             complement(7803..8576)
                     /locus_tag="PLB01_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77366.1"
                     /translation="MDLVGLDAPGPVIRVVLAPESSPLAREMPAWVTGYTDGVTSDAG
                     SVVVILAERTPSYPDGGLEEVLAHEVAHVLIHRAAAGRRVPRWFDEGLAMLAARTWRW
                     RDQTELALGLLSGPRVPLWKLDDLFEGDRRQVERGYALSGTLVQDLLERYGSAVPRIV
                     LARLAHGDTFEQAIRGATGATLIDVGEAFWDRQGTLKRWIPILTSTAVLWFGISVLAI
                     GAGIRKRRQRAAQAKAMEEAEIPAAGPDEEPTPRDETVN"
     gene            8816..9190
                     /gene="acpS"
                     /locus_tag="PLB01_08425"
     CDS             8816..9190
                     /gene="acpS"
                     /locus_tag="PLB01_08425"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQQ77367.1"
                     /translation="MILGIGTDLLDVARMARELEKDGAGFRDRVFTPAEIAYCEAMRY
                     PARHFAARFAAKEALLKALAGPASDDFWHEAEVERAGDAAPRLVLHGRAKEAADRLGV
                     KSILVSLSHTDSLAAASVVLQG"
     gene            9195..10931
                     /gene="acsA"
                     /locus_tag="PLB01_08430"
     CDS             9195..10931
                     /gene="acsA"
                     /locus_tag="PLB01_08430"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQQ77368.1"
                     /translation="MYVTETLEEKRLHNIGSYEERLRAFDWAIAEKEMSWGHGDPVNI
                     GWHLSDRLCRLGLGRKTALLWEGAGGNQRTYTFDDLRLLSNAVAAALTRFGLAPSERI
                     CLFLDRVPELYIGFVGILKMGAVAQPLFSAFGEESLATRLLDAGTAAIVTQKKHLPKV
                     RRIRAQLPALRTVVVVDAEGLALKAEDGEVAFDLDAEPRLERFHIFPAAPETASVLHY
                     TSGTTGQPKGAQHVHASLVSQYLTAKWVLDLKPDDVYWCNADPGWVTGTSYGIIGPWS
                     NGVTQAVLDSGFSADRWYGFIEKRKVSVWYSAPTAIRLLMREGTPAALKHDLSSLRHL
                     ASVGEPLNPEAVHWGCAAFGLPFHDTYWQTETGCIVISNYPGMPVKPGSMGKPFPGIT
                     AAVLDPATFEPQKQGRVGVIGLKPGWPSMIRGYWNNPAGYAKKFANGWYLTGDRATVD
                     ADGYFWFVGRDDDVINTGGHLVGPFEIESALLEHPAVAESAAVAKPDPVNMEVVKAFV
                     ALKPGYTAGDDLELEIMNFVRKRLSPLAMPQEIAFVASLPRTRSGKILRRVLRAPEWG
                     EPVGDLSTLMED"
     gene            10932..11189
                     /locus_tag="PLB01_08435"
     CDS             10932..11189
                     /locus_tag="PLB01_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQQ77369.1"
                     /translation="MDDLKKTILEYVRREYIEEGDDRELGTSTPLITGGIVDSFSMVS
                     LKRFLEKKYAIRIPDADASPAAFDSVDKIASLVERFTAVKV"
     gene            11191..12447
                     /locus_tag="PLB01_08440"
     CDS             11191..12447
                     /locus_tag="PLB01_08440"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQQ77370.1"
                     /translation="MTTATLPATYKEILGAELEALKNAGLYKEERFIHAPQGTEIDVE
                     FPAGASPKRVLNLCANNYLGLSSHPDVLAAAHLGLERRGYGMSSVRFICGTQDIHREL
                     ERRITAFLGTEDTILFSSCLDANAGVFEAILSDQDVMIADRLVHASIVDGMRLCKAAQ
                     DTYKHSDMNHLEEKLVEHKDKRLRLVITDGVFSMDGDLAKLDEIVALCEKHDAMVFVD
                     DSHATGFIGKTGRGTPERFGVHGKIDILTTTFGKALGGASGGCVSGRKEIVEMCRQRA
                     RPYLFSNTLASVIVAGTLKVLDIVSTHTERRDRLEQNAKTWRRLLTEAGFDIKAGESP
                     IVPVMLYNAKLAQDVARDLFAEGVYVVGFFFPVVAKGQARIRTQLSAAHTTAQLERAI
                     EAFRKVGKKHDILGKSKNEIVERYGL"
     gene            12468..12773
                     /locus_tag="PLB01_08445"
     CDS             12468..12773
                     /locus_tag="PLB01_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77371.1"
                     /translation="MHARRTLEVPIPDDVKLDGLDVELLKATFQALVALRGERDRRWE
                     LTLKQLEDDGWRVKWGLAWRAEAKRGEDYEEATGATLDEALAGVAQFARADMVGHAP"
     gene            12811..13143
                     /locus_tag="PLB01_08450"
     CDS             12811..13143
                     /locus_tag="PLB01_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77372.1"
                     /translation="MEPSRRRVLAARILAVAADAVQLGLLPLFAGGAATGFDAVLDVA
                     VGIVMVALVGWHWAFLPAFALELVPAVDLAPTWTIAVLIATRRMGEAEPPVPATRIDP
                     PPLPPARG"
     gene            complement(13150..14355)
                     /locus_tag="PLB01_08455"
     CDS             complement(13150..14355)
                     /locus_tag="PLB01_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ77373.1"
                     /translation="MRLGPHRILFAGPLAVAAVAVAALAAGAGETPARLEPWTKGTLD
                     VHQISTGRGNAALVVFPDGTTLLFDAGDAGDGVPLATALPDASRGAGEWVARYVKRTA
                     GEGAVLDYTVVSHFHPDHVSGLGRVAALLPIHRLIDRGFPGYGEPAAPKGAVFDDYRR
                     VAEDAARKGTVRERARPGRADQIVPVRAPGDAAAFSVRVVAANGEVWTGRGDATARRF
                     PDLSGVPAEDRPSENPCSVALLIRYGGFSLYTGGDLYGAPEPGAPAWWDVETPIANAI
                     GKTDVMVVDHHGSIEPASPFFLATLAPRVIVVPAWSPTHPSPDVLKRLLSKRIWPAPR
                     DVFVTRLRDATKAAIGPRATQVASDAGHVVVRVAPGGASYRVFVLDDTNESGAVKSVH
                     GPYASYGAR"
BASE COUNT         2195 a         5240 c         4987 g         2089 t
ORIGIN      
        1 cggcgcgacg tcgagaacgg ccacgccgtc gcgcaggaga tacgccccgc gcagcgtcca
       61 tccctcggga aacggccgca tgaggtccgc gctgcgcggt ccctcgacga cgccggacgc
      121 caccgaccgc aggaacgcga cgtcgtccgc cgcgacgggc acgtcgcgcg cctcggggtg
      181 cagcacgccg tcgccctccg tcgactcgaa gaagagcgtc acgttgcgcg cggggatggg
      241 cgtcggaggc acgacggccg tcgggcgcgg cgcgggcacg ggcggcgcgc cggccttcgg
      301 gttccggacg ctccgcatga cgaggaacgc gacgaggagc agcccgagga tcgcgacgag
      361 cgcgagcgcg aagcggcgcg ccacgggact cagctcttgc cgttcttcgg cgcgtacggc
      421 acgggtgcgg cgccgtcggc ggaccggaag tacgtctcga ggctgcccgc gagcgtgtcc
      481 gccacctggc ggcggaattc ctcggagttg atcttcctct cctcgtcggg attcgtgatg
      541 aacgacacct cgacgagcac ggcgggcgcg ttcacgccga tgaggacccg gaagggcgcc
      601 tgcttgacgc cgcgcgtcgt gatcccgaga aggcggttga agtcggcctg cacgatctcg
      661 gcgaggcgcg cggacgcgcc gagatgctgg ttctgcgcga ggtcccagag gatgaggtcg
      721 aggtcgcgga ccgcggcgtt cttttccgcg gaggccgcgc cgggcgcggc gcggttctcg
      781 ctctcggcga gcgcggccgc cgcgcggtcc gacgcgtcga gcgagaggta gtagacctcg
      841 gtcccgtgcg cggaggccgc gcgggacgcg ttgcagtgga gggagaggaa cacgtccgcc
      901 ttcgcggcgt tcgcggcggt ggagcggtcc tcgaggccca ccgtcgcgtc ggtcgtgcgc
      961 gtcaggacga cgcggtagcc gcggcgctgg agcgtctcgg cgaccgtctt gcccagcgcg
     1021 agcgtcgcgt ccttctcgaa cgaaccgccc gggcccttcg ccccttcctc ggtcccgccg
     1081 tggcccgggt cgatcacgat cgtcttcgcg gtcttcgcgg cggcgggcgc cgggaccggc
     1141 gcggccggcg ccgcggaggg cccggatccg gccctgcgcg tgacgtccac gacgaggcgg
     1201 ggcggcgtgc cgagcgcgta gacgttcgcc gcgaggccct tctcccggaa gacgacggtc
     1261 acctcgccgc cgcgcacgag gagccgcgcg acgcgcggcg agtcgaggac gcgctcgggg
     1321 ggcggcccgg tgaggcgcgc gcccgggaag tgcaggacga cctgctcggg cgacgtctcc
     1381 accgcgtacg agggcgactg cgagaacctc aggacgatct tcgtgacgtc gccgagctcc
     1441 gcgacggaag gctccacgcc gacctcgacg aactccgact tccgggccga gagcgtgcgc
     1501 gtcgccttct cccacgcgag ggcctggccc gagagcggcc cgagcacccg gacgaagaac
     1561 tcgggctcgg cccacgcgcc gaggctcgac gcccggacgg gggacgcgag gttcacgacc
     1621 ttcgtgtcca cgacggcgat cggcgactcc gcgccgaaga ccgccgtgcg gccctttacc
     1681 ttcatttcgt acgagcgcgt ccgttcgtcc caggtgatcg tcccgtcgag ggcggcgacc
     1741 gcgtcgttca cgaggaggta cgtccgcccg tctcgctcct cggcgcggat cacggcggcg
     1801 ggcttgccgt cccgcgcgag cgatgcgtct tccccgtgcg ccgcgcggga gccgaacagc
     1861 gaggagagcg ccgcggcggc gagaaggatc aggagccggg ccgtcgcgcg cgtcacccgc
     1921 gaattatggc gactccggga cttttccgcg cccccgacct gttatccttg gtgtcacggt
     1981 cgggggcgtc ccggtttcga cggggcattc agcgatcgag gagcacgccg agcacggccg
     2041 ttcgctcgta aaaacgacgg tcaaattcaa tcgccaacag cgattacgct ctcgcggctt
     2101 aattagccac ggcgggcgcc ggggagcctg tccggggcgc ctcggcgctt gacgcgaaat
     2161 ccggactggg gatcatctcc gccgcctgaa gagatggccc gagaagctca ggatcgctgc
     2221 cgcgcccggt cgtccggacc tgaagacgcg gtggctaaaa aaacaagtcc ggactgagcg
     2281 tggagaatct cgacgttcgg gcgcttcgga cgggggttcg actccccccg cctccaccat
     2341 atcgaggggg gatccgaaaa acggatcccc cctcggcctt ttcaccgtcc tcgtctaccc
     2401 gaggtgtctg cgacctaaat gccaccatcc cggatgccgg gtacccccgt cgcgggacca
     2461 cggattccgg cgtccatccc ctggttgacg cccagcggct gcggatcggg ctgggtcatg
     2521 acggccctcc acccgccgat gccggtgtga tagcggatac ggttccgcca cggccacgaa
     2581 tgccagtatt ccactcggca tccccacccc atcaggtcgc cgcgctgcca tgcggtcgtc
     2641 ccgtagcatg caactgcgtt gtcgacgggg cagttggcgg ggtcacgagc gacgtgcgag
     2701 aggccgagca gatgcccgac ctcgtgcagg gcggtcatgt gaatctggac ttcgcccggc
     2761 acgggctgca cgtcctcgta gctcgcatcg actgcgttca attcgccgga gtggttcccg
     2821 ggcgacacgc ccgagcgcat ggcctgcccc gggaccgggt agatgcagtt cacgatgatt
     2881 ctggcgtgct cgggccggtc gcgtatccag agagtcaacc cgcacgctac gtttgggcgg
     2941 tgggtcggcc ggccctcggg ccagtcgaga ccggtgtacc cccggggcgg gatgagccag
     3001 aacctcctgg aaaaaaagcc cgtgacctcg cggtagaacc tcccgcggaa atccctccac
     3061 tcgttgtcgg gccagcgcag gatctgggtg atccctcccg aggcgtcggg atacaggccg
     3121 gaagagtcgg gctcgacgcg attgaggtag atgctgagga tgaagttgag gtcggcgttc
     3181 cggcccgcat gacgttcgag acacgaatcg agcaccggcc cgcggatcca ttcacgcaac
     3241 atggcccctc ccgacggctc attctcgaaa gctcaagtca gcgtgcgatc accttccaaa
     3301 gccacgccgc gcagcgtgct cttcgtcgcc cacgtcgcgc ccgcgtcgat tcgatgagat
     3361 tcctactcta ctccggccgg cgacgtcgtt ggggacggct gtagagtacc cgcggagggc
     3421 tgcatgcgat cgaccgtcct cgccctgtgc ctcggtgccg ccctcgtctc cgccgcgctt
     3481 cccgcgcacg cggtccttcc ccccggcgtc gacgacgcct cgatcgcggc cgccgccgcc
     3541 gcgaccttcc cggagttcct cgaccttctc gccctcccga acgactccgc gaagcccgcg
     3601 gacatccaga agaacgccgc gtggctcgag gccgcgttcg cacgccgcgg gttcgccacg
     3661 cgccagatcg aaaacaacgg ccgcccgctc gtcttcaacg agtacggcaa ggcccggccg
     3721 gcgcagaaga ccgtcctctt ctacattcac ttcgacgccc agcccgtcgt cccctcgcag
     3781 tgggcgcaga aggacccgtg gctgcccgtc gtcaagcgcc gcggcgcgga cgggacgtgg
     3841 gcggaggtcg accgcgccga gcttctgaag ccggatttcg accgcgacct gcgcgtcttc
     3901 gcgcgctcgt cctccgacga caagggtccg atcgtcatgt tcctcgcggt cttcgatctc
     3961 ctgcgcgcga agaagatcga tccggccttc cacgtgaagg tcctcctcga cagcgaggag
     4021 gaggccgggt cgccgggcat cgccgccgcc gtcgcctcga acgccgcgct cctctcggcc
     4081 gacgccgtcg tcctcctcga cgggccggtc cacgcctcgg accggcccac gctcgccttc
     4141 gggaaccgcg gcgtcgtcgg cgtcacgctc accgtctacg ggccgcgcgc gcctctccac
     4201 agcgggcact tcggcaactg ggtgcccaat cccgcgctgc gtctcgcgaa gctcctcgcc
     4261 tcgatgaagg acgaggacgg gcgcgtgacc gtcgccgggt actacggccg cacgaacctc
     4321 accgacgcgg accgccgggt gctcgccgag gtgccggacg acgaggccgc cctcaagaag
     4381 cggacgggca tcgcgcagcc ggagaaggtc ggcgcaacgt accaggagtc gctgcagtac
     4441 ccgtcgctca acgtgcgcgg cctcgcgtcg gcgagcgtgg gcgagaaggc cgcgaacgtc
     4501 ctcccgcgcg acgccgtcgc cgagctggac ctgcgcacga cgcccgagtc cgacccggcg
     4561 ttcctactcg gcctcctgaa gaagcacgtc gaggcgcagg gctggcacct cgtcgagaag
     4621 gacccgaccg acgacgagcg cgcgcgctgg ccgaagctcg cgcgtttcga cgcgcacgct
     4681 cccgaggagg ccgcgcgcca ggcgttcgaa gcgcccgtgg gccgctgggc agccaacgcc
     4741 gtctccggcg cgtggccctc cgcgccgctc gtgcgcatcc ggatgatggg cggaacgctc
     4801 ccgacgcacg cgatcgtcgg gcctctcaag gcgccgttcg tgcaggttcc cgtcgtgaac
     4861 ccggacaata accagcacgc gtacgacgag aacctgcgca tgggccatta cctgagcggc
     4921 atgcggacga tggcggcgct cctcacggcg ccgtacccgg agtgagagag aatccgtctc
     4981 atgcgaaacc accgccgtct cccgttgatg ctcgcgttct gcctggccgc tcccgccttc
     5041 gcgcagggac gacctgatcc ggcggcgctc ctcgccgcgc agaaggacgc gctgaaggcc
     5101 ttcgcggcga tggacggcgt gtggcgcggc cccgcgtgga cgctgctccc ctccggcgag
     5161 aagcacacga tcacgcagac tgagcgcatc ggcccgttcc tggacggctc cgtgaaggtc
     5221 atcgagggca agggctacga ggccgacggc cgcgtctcct tcaacgcgtt cgggaccatc
     5281 tcgttcagcc ccgcgaagaa cgcgtacacg ctccactcgt acgcgatggg cagcgtcggg
     5341 gatttcgtca tcaagcccac gccggacggc tacacgtggg agatcccggc cgggccaatg
     5401 accatccgct acacggccgt catcaaggac ggcacgctca aggaagtcgg cgaccgcctc
     5461 gagccgggca aggatcccgt ccgcttcttc gagatgacgc tcacgcgcat cggcgacacg
     5521 acgtggcccg ccggcggcgc ggtgccgccg aagtagcggc cttcgtgaac gacctcgccc
     5581 gccgcgattt cctgcgcttt ctcgcggcga gcccgctcct cgccgggctc ggcggcctcg
     5641 acgcgttcgc cgacggaggc gagccgctcg cggactccgc cgggaaggcg ctcgacgtct
     5701 tcgagctcga ggcgatcgcg aagaagaacc ttccgcccgc ccactggggc tacatggcga
     5761 ccggcgtcga cggcgacgcg acgctgaggg cgaacagcga gggctacgcg aacctcgcgc
     5821 tgcgcgtccg ccgcctcgcc ggcgcgcaca agcccgacac gtccgtctcg ctcttcggca
     5881 cggcgtggga gacgcccatc gtcctctgcc ccgtgtccag ccagcacgcg ttccacgagg
     5941 acgccgagat cgcgaccgcc cgcgccgcgc gctcgcgcag gaccctgcag atcctttcca
     6001 cgctctcgtc caccggcgtc gaggacgtcg ccgccgcgcg cggcggcccc gtctggtacc
     6061 agctctatcc gaccgaccag tggtccgtgg cgcagggcct catgaagcgc gccgaggccg
     6121 ccggctgccc cgcgctcgtc ctgaccgtcg acctgcaggg cggatccaac cgcgagaccg
     6181 tcgtgcgcgg ccgccgcctc gacacgcgcg actgctcgtc ctgccacgtc gccgacccgt
     6241 tcgacgtcgc cggcgcgctc ccccgccggg cgatgttcaa ggggctcgac gtttcgaagg
     6301 tcacgtggat cttcccgccc gacgccacgt gggactacgt ggcgaagatc cgcgccgcct
     6361 ggccccggaa gctgctcctc aagggcatcg tcacgcgcga ggacgccgag ctctgcctcg
     6421 cgaacggcgt cgacggcatc gtcgtctcga accacggcgg ccgcgccgag gaaagcctgc
     6481 ggccgtcgat cgacagcctc gtcgaggtcg cggcggccgt gaagggccgc gtccccgtga
     6541 tcctcgacgg cggcatccgc cgcgggacgg acatcttcaa ggcgctcgcg ctcggcgcga
     6601 ccgccgtcgg catcgggcgc ccgcaggtct ggggcctcgc cgcgttcggg caggccggcg
     6661 tcgaggccgc gctcgacatt ctccggcgcg agctcgagat ggtcatgcgc caggcgggga
     6721 cgacgagcct cgcgaagatc gaccgctcgt acgtcgtcga ccggcggcgc gccccggcag
     6781 gctagctctt cgcgagcgcc ttcaggatgt tgacgaccat tccgggcagc cgccctgggt
     6841 gcgcctcgac cgaggcgagg acgtccccga tcgtcgtggc gccctcgagc tcggcccact
     6901 tcgcgacctc cgcggcgctc ggcgagaggt acgccatgct ccacgagtcg aacgcgcgct
     6961 cgtcgacgtc ctgctcgtag aacaccttca cgccgcggtg ccgcgggtcg cggtcgatcc
     7021 ggctcatcag gtcgacgacg tcgttcttct cgccctcgag gacctggagg aacgcgccgt
     7081 cgacgaagac gaggacgccc gagatcccgt gcgccttgtt gcggatccgc gactccgcga
     7141 gcatcccttc gagttcggcc ggcgtcagat ccggcgccgc ttcggaggaa tagacgaggc
     7201 gatacggcat gggcaccctc ccgaggaggg gagtctattc cggaaaaaga aaacggcgcc
     7261 ggagcgtttc cgcccggcgc cgaatttgaa cccggtcgaa gcgttactta gtaacggttg
     7321 cggccgccgc cgaagccgcc gccaccaccg cggttctcct ggggcttggc ctcgttgacc
     7381 ttgagcgcgc ggccgtcctg ctcgcggccg ttgagggcgc ccatcgcctt cttggcgtcc
     7441 tcgtcggaca tctcgacgaa gccgaagccc ttcgaggagc cggtgtcgcg gtccgagatc
     7501 acgcgggcgg attcgacgtt gccgaagggc tcgaacaggg acttgagggt ggagtcgtta
     7561 acggaatagg acaggtttcc aacgtagagc ttcatgagat tccttagaaa aacgcgcggc
     7621 cgattggacg tgcgcgactg agagatggtg gggagggtac ggttgaggtc gacggggacc
     7681 tttcggttct cggtccgctc cgtcacatag aaaggagtgc gaacggggga agtatagggc
     7741 ctttcccgtt ttcctgcttg gaatacgaca caacccgaac gcggagagcc acttaggggg
     7801 tttcagttca cggtctcgtc ccgcggggtc ggctcttcgt ccgggcctgc cgccggaatc
     7861 tccgcctctt ccatcgcctt tgcctgcgcc gcgcgctgcc tcctcttccg gattcccgcg
     7921 ccgatcgcga gcacggagat cccgaaccac agcaccgcgg tactcgtgag gatggggatc
     7981 cagcgcttga gcgtgccctg ccggtcccag aacgcctcgc cgacgtcgat cagcgtggcg
     8041 cccgtcgcgc cgcgaatcgc ctgctcgaac gtgtctccgt gcgcgaggcg cgcgaggacg
     8101 atgcgcggca ccgcggagcc gtagcgttcg aggaggtcct gcacgagcgt tccggacagc
     8161 gcgtacccgc gctccacctg gcgccggtcg ccctcgaaca ggtcgtccag cttccagagc
     8221 ggcacgcgcg ggcccgacag cagccccagc gcgagctccg tctggtcgcg ccagcgccac
     8281 gtgcgcgccg cgagcatcgc gagcccctcg tcgaaccagc gcggcacgcg ccgcccggcc
     8341 gccgcgcggt gaatgaggac gtgcgccacc tcgtgcgcga gaacctcctc gaggccgccg
     8401 tccggatacg acggcgtgcg ctcggcgagg atcacgacga cgcttcccgc gtcggacgtc
     8461 acgccgtccg tgtatcccgt cacccacgcc ggcatctcgc gcgcgagggg cgacgactcc
     8521 ggcgcgagga cgacgcggat gaccggcccc ggcgcgtcga gccccacgag atccatcacg
     8581 ctccacatcc acggccgcgt ccccgcggcg aggcggttcg ccgagggcgg cagacccggc
     8641 tgggggtcga agacgaggcg tgtcggctcc agggccgcga cgggcgaggc gagaagaagg
     8701 acggcgagga gagcggattt ccggatggcg cccctagcgt acccgcatga gcaggttgaa
     8761 tcatggcggc gggcggccgg gggacccagt tgtctaggca ggaagagcta cgcccatgat
     8821 cctcgggatc ggcacggacc ttctggacgt cgcccgcatg gcgagggagc tggagaagga
     8881 cggagccggg ttccgggacc gggtcttcac gccggctgaa atcgcgtact gcgaggccat
     8941 gcggtacccg gcgcggcact tcgccgcccg cttcgcggcc aaggaggcgc tcctcaaggc
     9001 gctcgccggc cccgcgtccg acgacttctg gcacgaggcc gaagtcgagc gggcgggcga
     9061 cgcggccccg cgcctcgtcc tgcacggccg ggcgaaggaa gcggcggacc gcctcggcgt
     9121 caagagcatc ctggtttccc tttcgcacac cgacagcctc gccgcggcga gcgtcgtgct
     9181 tcaggggtga acgcatgtac gtgacggaga ccctcgaaga aaagcgcctg cacaacatcg
     9241 ggtcgtacga ggagcggctc cgggcattcg actgggcgat cgccgagaag gagatgagct
     9301 ggggccacgg cgatcccgtc aacatcggct ggcacctcag cgaccggctc tgccgcctgg
     9361 gcctcgggag gaagacggcg ctcctctggg agggcgccgg cggaaaccag cggacgtaca
     9421 ccttcgacga cctgcgcctc ctctcgaacg ccgtcgccgc cgctctcacg cgcttcggcc
     9481 tcgcgccgtc cgagcggatc tgtctcttcc tcgaccgcgt gccggagctc tacatcggct
     9541 tcgtcggaat cctcaagatg ggggcggtcg cccagccgct cttctccgcg ttcggcgagg
     9601 agtcgctcgc gacgcgcctc ctcgacgccg gaaccgcggc catcgtcacc cagaagaagc
     9661 acctgccgaa ggtccgccgc atccgcgcgc agctcccggc cctccgcacc gtcgtcgtcg
     9721 tcgacgcgga aggcctcgcg ttgaaggccg aggacggtga ggtcgcgttc gacctcgacg
     9781 ccgagccgcg cctggaacgc ttccacatct tccccgcggc gcccgagacg gcctcggttc
     9841 tccactacac gtccggcacg accgggcagc ccaagggcgc gcagcacgtc cacgcctcgc
     9901 tcgtgtcgca gtacctgacg gcgaagtggg tgctcgacct caagcccgac gacgtctact
     9961 ggtgcaacgc ggaccccggc tgggtgaccg ggacgtcgta cgggatcatc ggcccctggt
    10021 cgaacggggt gacgcaggcc gtcctcgact ccggcttcag cgccgaccgc tggtacggct
    10081 tcatcgagaa gcggaaggtc agcgtctggt actcggctcc cacggccatc cgcctcctca
    10141 tgcgcgaggg gacgccggcg gcgctgaagc acgacctctc ctcgctgcgg cacctcgcga
    10201 gcgtcggcga gccgctcaac ccggaggccg ttcactgggg gtgcgcggcg ttcgggctgc
    10261 cgttccacga cacgtactgg cagacggaga ccggctgcat cgtcatctcc aactatccgg
    10321 ggatgccggt caagccgggc tcgatgggca agcccttccc gggcatcacg gcggccgtcc
    10381 tcgacccggc cacgttcgag ccgcagaagc agggccgcgt cggagtcatc gggctcaagc
    10441 cgggctggcc ctccatgatc cgcggctact ggaacaaccc cgcgggctat gcgaagaagt
    10501 tcgcaaacgg ctggtacctc acgggcgacc gcgccaccgt ggacgcggac ggctacttct
    10561 ggttcgtggg ccgcgacgac gacgtcatca acacgggcgg gcacctcgtc ggcccgttcg
    10621 agatcgagtc ggcgctcctc gagcacccgg ccgtggccga gtcggcggcc gtcgcaaagc
    10681 cggaccccgt caacatggaa gtcgtcaagg cgttcgtcgc gctcaagccc ggttacacgg
    10741 ccggcgacga cctcgagctc gagatcatga acttcgtccg caagcggctc tctcccctcg
    10801 cgatgccgca ggagatcgcc ttcgtggcgt cgctcccgcg gacccggagc ggaaagatcc
    10861 tccggcgggt cctccgcgcc ccggagtggg gcgagcccgt cggcgacctt tccaccctca
    10921 tggaggactg aatggacgac ctcaagaaga cgatcctcga atacgtccgc cgcgaataca
    10981 tcgaggaggg ggacgaccgc gagctcggga cctcgacgcc gctcatcacg ggcgggatcg
    11041 tcgactcctt ctcgatggtc tccctcaagc gtttcctcga gaagaagtac gcgatccgga
    11101 ttcccgacgc cgacgcctct cccgccgcct tcgacagcgt cgacaagatt gcctccctcg
    11161 tcgagcggtt caccgccgtg aaggtgtgac atgacgaccg ccactctccc cgccacgtac
    11221 aaggaaatcc tcggcgccga gctcgaagcc ctgaagaacg ccggcctcta caaggaggag
    11281 cggttcatcc acgcgccgca gggcaccgag atcgacgtcg agttccccgc cggcgcctcg
    11341 cccaagaggg tcctgaacct ctgcgcgaac aactacctcg gcctttcgtc gcacccagac
    11401 gtgctcgcgg ccgcccacct cggcctcgaa cggcgcggct acggcatgtc gtccgtccgc
    11461 ttcatctgcg gcacgcagga catccaccgc gagctcgagc ggcggatcac ggcgttcctc
    11521 gggaccgagg acacgatcct cttctcgtcc tgcctcgacg cgaacgcggg cgtcttcgag
    11581 gcgatcctct ccgaccagga cgtgatgatc gccgaccgcc tcgtccacgc gtcgatcgtg
    11641 gacggcatgc gcctctgcaa ggccgcccag gacacctaca agcactccga catgaaccac
    11701 ctcgaggaga agctcgtcga gcacaaggac aagcgcctcc ggctcgtgat cacggacggc
    11761 gtcttctcga tggacggcga cctcgcgaag ctggacgaga tcgtggccct ctgcgagaag
    11821 cacgacgcga tggtcttcgt ggacgactcg cacgccaccg gcttcatcgg caagacgggg
    11881 cgcggcacgc ccgagcgttt cggcgtgcac ggaaagatcg acatcctcac gacgaccttc
    11941 ggcaaggcgc tcggcggcgc ctcgggcggg tgcgtcagcg gccggaagga gatcgtcgag
    12001 atgtgccgcc agcgcgcgcg gccgtacctc ttctcgaaca cgctcgcctc ggtcatcgtc
    12061 gccggcacgc tcaaggtgct cgacatcgtg tccacgcaca ccgagcggcg cgaccggctc
    12121 gagcagaacg cgaagacctg gcggcggctc ctgacggagg ccggcttcga catcaaggcg
    12181 ggcgagagcc ccatcgtccc cgtcatgctc tacaacgcga agctcgcgca ggacgtcgcg
    12241 cgcgacctct tcgccgaggg cgtctacgtc gtcggcttct tcttcccggt cgtcgccaag
    12301 gggcaggcgc gcatccgcac gcagctctcg gccgcgcaca cgaccgcgca gctcgagcgg
    12361 gccatcgagg ccttccgcaa ggtggggaaa aagcacgaca tcctcggcaa gtcgaagaac
    12421 gagatagtgg agaggtacgg cctctaggcc gagacggagg aggcgccatg cacgcccgca
    12481 ggacgctgga ggtcccgatt ccggacgacg tgaagctcga cggcctcgac gtcgagcttc
    12541 tgaaggccac gttccaggcg ctcgtcgccc tccgggggga gcgggaccgc cgctgggagc
    12601 tgacgctcaa gcagctcgag gacgacggct ggagggtcaa gtggggcctc gcctggcggg
    12661 ccgaggccaa gcgcggcgag gactacgagg aggcgacggg ggccacgctc gacgaagcgc
    12721 tcgcgggggt cgcccagttc gcgcgcgcgg acatggtggg ccacgcgccg tgagttgacc
    12781 gtgctgcgcg ttcgggccta acgtccgcgc gtggagccgt cccgccgccg cgtcctcgcg
    12841 gcccgcatcc tcgccgtcgc cgccgacgcc gtgcagctcg gcctcctgcc gctcttcgcg
    12901 ggcggcgcgg ccacggggtt cgacgccgtg ctcgacgtcg ccgtcggcat cgtcatggtc
    12961 gcactcgtcg gctggcactg ggccttcctg cccgcgttcg cgctcgagct cgtgcccgcc
    13021 gtcgacctcg cgccgacgtg gacgatcgcg gtcctcatcg cgacgcggcg catgggcgag
    13081 gccgagcctc ccgtgcccgc gacgcggatc gacccgccgc cgctgccgcc cgcgcgcggc
    13141 tgagaagggt cagcgcgcgc cgtacgacgc gtacgggccg tgcacggact tcacagcgcc
    13201 gctctcgttc gtgtcgtcga ggacgaagac gcggtacgac gcgccgccgg gcgcgacgcg
    13261 cacgacgacg tgccccgcgt ccgaggccac ctgtgtcgcg cgcgggccga tggccgcttt
    13321 cgtggcgtcg cgcaggcgcg tcacgaagac gtcgcgcggc gcgggccaga tccgcttcga
    13381 gagcagccgc ttgaggacgt ccggcgacgg gtgcgtcggc gaccacgccg gcacgacgat
    13441 cacgcggggc gcgagcgtcg cgaggaagaa cgggctcgcg ggctcgatcg agccgtgatg
    13501 gtcgacgacc atgacgtccg tctttccgat cgcgttcgcg atcggcgtct cgacgtccca
    13561 ccacgcgggc gcaccgggct cgggcgcgcc gtagaggtcg ccgcccgtgt agagcgagaa
    13621 gccgccgtac cggatcagca gcgcgacgct gcacgggttt tcggaaggcc ggtcctcggc
    13681 gggcacgccg gaaaggtccg ggaaccggcg ggcggtcgcg tcgcccctgc cggtccacac
    13741 ctcgccgttg gcggccacga cgcgcaccga gaacgcggcc gcatcgcccg gagcgcgcac
    13801 ggggacgatc tggtccgccc ggccggggcg cgcccgctcg cggacggtcc ccttcctcgc
    13861 ggcgtcctcg gcgacgcgcc ggtaatcgtc gaacacggct cctttcggcg ccgcgggctc
    13921 gccgtagccg gggaagccgc ggtcgatcaa cctgtggatc ggcagcagcg cggcgaccct
    13981 gccgaggccg gacacgtggt ccggatgaaa gtgcgagacg accgtgtagt cgaggaccgc
    14041 gccctcgccc gccgtgcgct tcacgtagcg ggccacccac tcccctgcgc cgcgcgacgc
    14101 gtcgggcagc gcggtggcga gcgggacgcc gtccccggcg tcgcccgcgt cgaagagcag
    14161 cgtcgtcccg tccgggaaga cgacgagcgc cgcgttgccg cggcccgtcg aaatctggtg
    14221 gacgtcgagc gtgcccttcg tccacggctc gagccgggcc ggagtctctc cggctccggc
    14281 ggcgagcgcg gcgacggcga cggccgccac ggcgagcggc ccggcgaaga ggatccggtg
    14341 cggtccgagt cgcatgtgcc ctcgttacgc cgggaccggc ggcggggttt ttcgcctgag
    14401 gatcgccgac gacaccagcg tcacgagaag gcccatcggc agcacctcca ggaacgtcat
    14461 cccggcgttg aagagcgggt tccggtacag ctccttgaac ttcgccatct c
//
LOCUS       DAOMFY010000080        14483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1277_length_14483_cov_8.205018, whole genome shotgun sequence.
ACCESSION   DAOMFY010000080 DAOMFY010000000
VERSION     DAOMFY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14483
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1277_length_14483_cov_8.205018"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..441
                     /locus_tag="PLB01_08460"
     CDS             40..441
                     /locus_tag="PLB01_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQQ77374.1"
                     /translation="MRRHEFVAAALGAAALLLLAAAAFAAGRPSLECYFQTTLHDPVY
                     QKKTYEKVAAKWKTPAASEVPAVGKKTVVQAVIAKDGALATTEVSMSSGKKGWDAAAL
                     KAVKAAAPFDPLPAGFAYPTVQVHFHVSVVP"
     gene            complement(448..1644)
                     /gene="kbl"
                     /locus_tag="PLB01_08465"
     CDS             complement(448..1644)
                     /gene="kbl"
                     /locus_tag="PLB01_08465"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQQ77375.1"
                     /translation="MTSLIDYCRAEIESLKAAKTWKKERVLEGPTGARVNVDGRSVLM
                     LTSNNYLGFANHPRVVDAARNALGRWGNGLGSVRFICGTQQLHKDLEAAISKFFGTED
                     TILYMSCWNANEGLFAPLLGEEDALLSDALNHASIIDGIRLCKAKRYLVPHGDLAALE
                     TALKESQAQRRRLFITDGVFSMEGEIGPLGAFADLCERYDATMVVDDSHATGVLGKTG
                     RGSAEELGVMEKVKIFTSTLGKAMGAAAGGFTTGPAPVVDLLRQRSRTSLFSNALPPA
                     VAAGSLEAFKMLMEDPAPALRVQKNARTFRAKMKAAGFRIPEGIHPIVPVIVGDTAKA
                     LAMSNALFEKGVYVSGFGFPVVPQGHARLRCQISAVHTDDDLQEAVDAFVAVGREFGV
                     VSGTSS"
     gene            complement(1722..2852)
                     /locus_tag="PLB01_08470"
     CDS             complement(1722..2852)
                     /locus_tag="PLB01_08470"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HQQ77376.1"
                     /translation="MTFVSLPPRTACPNIRSKPTALAVVALVLSVPAVFAMPALAQGT
                     MGTAMIPIAAPASTVDSVVLKVNGPILDVLGGALQIDVTNARITGGEDRLASPLPWAG
                     ILVGSRIVAHVTVPDAIPAVFPPRLPATSVVVFLTNSGSLSGTVQGVGAAQGTFTLLY
                     TSVKTNAGTEWSGSKADGSPVKGLSDLAIGMQAVTVVSTDASGVTAKSVHAYTIPTTR
                     IEAFRGKVEKIDASAWTVDGKFVQVNADTKIVGDPKVGDVVDVLAKVTIVPPGMGMPS
                     MPPVAISIVKVISTPPPPTDRSVEFDGVVESMPPGLANSAGAALGHWKISGKDVLVTP
                     ATRVASGIGVGSSVHVKGVTLPMAVFAPTASPQILATEITKK"
     gene            complement(2919..3518)
                     /locus_tag="PLB01_08475"
     CDS             complement(2919..3518)
                     /locus_tag="PLB01_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ77377.1"
                     /translation="MFLALRRIHVFASDFSRSRRFYGETLGLPEAGGFENDWVEFALG
                     PLLLKLTPLRPSEVPARNQVAAVFSTASLDDAIARLKERGVTVTRPPADEFTGRSAEI
                     LDPDGYRIILFQASELHPDEEWVPASGVDELVKQKLNARKKREQARGTGKKPAKKAAS
                     RKPAKKAAAKKVKKAPAKKAAKKATRKKPAPKKKPGRRR"
     gene            complement(3579..4028)
                     /locus_tag="PLB01_08480"
     CDS             complement(3579..4028)
                     /locus_tag="PLB01_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQQ77378.1"
                     /translation="MSHLTESPVEPAGLLAAVRRDGDGGLALFVGVVRDHNDGKRVTR
                     LEYSAYIPMAEKEMDRIATEIEEAHPGARVAFRHRIGSLDIGDVAVAVAAAAPHRDEA
                     FAACRAGIEAIKARVPIWKKEFGPDGSSWVEPCGAPSHAPYGNPDST"
     gene            4053..4817
                     /locus_tag="PLB01_08485"
     CDS             4053..4817
                     /locus_tag="PLB01_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQQ77379.1"
                     /translation="MTPRRRRILAVSALKGGVGKTTTAVNLAAAAARAGVRTLLVDVD
                     PQGSVATSLGLPPSAGLSKWLLKKVDFEEAVVRGSRPNLDVLPAGEDLLGAEDALREK
                     GKEKRVGRLARRFASLEDEGYEAIVLDCPPAAGLLLENVYHAADEILLPAKLDFLSLP
                     ALEKTRVFVKHANETREKALKIDGVLPTFYDLRTAVSSELLDTLRERFSKILTPIRIN
                     VALAEAPSVGKTIFEFDGSARGAVDYALLAEELKLD"
     gene            complement(4814..5056)
                     /gene="moaD"
                     /locus_tag="PLB01_08490"
     CDS             complement(4814..5056)
                     /gene="moaD"
                     /locus_tag="PLB01_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HQQ77380.1"
                     /translation="MRVDVLLFASLRDELGPQVSVEVAEPATVESLRKALEAAYPAIA
                     RFGGRAKAAVNETWARETDAVKNGDTVALLPPVAGG"
     gene            complement(5086..7044)
                     /locus_tag="PLB01_08495"
     CDS             complement(5086..7044)
                     /locus_tag="PLB01_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77381.1"
                     /translation="MSNRTFPLLLCLLVGASSATASEVLAVAHPAGTPATTTAAGGSF
                     LTNDPFSGPTPSCFSGDARSLTFTSTANDLVPSQAGNLFGAVFVTDLSNDTVQIVSHS
                     ASSFSSTANGPSDSPVISEDGRWIAYLSAATDLVTGQTGNGYTYNVVLFDRSTGANFL
                     VSHATGLPGAGGNADSSAPAISADGRWVSFLSRATDLVPGEVVGHPPPGADLFLFDRV
                     TTSLTLVSHRAGFPSRTGDGSTQWHAMSADGRFVSFASSAGDLVPGQIDWQGTSDVFL
                     FDREAGTISLVTRRVGTSATAGNNQSFAPSISADGRFIAFFSVASDLVAGLTDTIPGW
                     PNVYCFDRLAGVSVLVSHAPGLPLTTADSGSFDPRISADGRAVAYCSAATSLVPGQQD
                     SPGLSNDVFVWDRLTGTNTLVSHKAVGATQTGSFESRNPEISADGSRVAFISRATDLV
                     PSQVDGNDTYDVFLHDRRTSRTMLVSRKFADQAATGSLGSSGPRLSADGNLVAFNSDA
                     SELVASDANGLPDAFLFRVASFGGATRLSLLPPCRAIDTRTPGGALPPNSLRDFTLFG
                     LCGIPTDAISVSANVTVTGTESPGFLGVFPAGLAVPATSVLNFSAGQTRANNAIVSIG
                     GTPSGTITVFNSSGGNVDVIVDVNGYFR"
     gene            7124..7909
                     /locus_tag="PLB01_08500"
     CDS             7124..7909
                     /locus_tag="PLB01_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ77382.1"
                     /translation="MSQGRLAGKNALVTGANKGIGRAIALRLAAEGARVAVAARKEAE
                     AEAVAAEIVKAGGEAHAVALDVTDAASVATGVERAARPDRRIDVLVNNAGLGGPSPLD
                     GDVRSDEAWHAILEVNLTGVWRVLRAANPFLAPGGRVVNLSSVTGRFGVAGMAAYSTT
                     KHGIIGLTRTLALELAPRKITVNAICPGWVDTEMGRSGIARMGKALGKSPDEAFAFAG
                     KMAPLGEVLTPDEIGGLVVYLASDDARNVTGQALVIDGGQVMP"
     gene            7915..8610
                     /locus_tag="PLB01_08505"
     CDS             7915..8610
                     /locus_tag="PLB01_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQQ77383.1"
                     /translation="MRAVFFDAGDTLLRADPPVEEVYRGAFARYGVRAQAEEVHAAVH
                     ATWRDVDLARERGEERWGGAGGEAGFWRRFVGTVFTRVGGGAMPEPLLGELVAHFGAE
                     RHWAVYPETRGVLAALRAAGLRLVIVSNWDSTLPSLLERLGLAAAVDDVVVSALLGVS
                     KPARGIFDEAVRRAGVTHAEALHVGDSLTDDYHGARDAGLSSLLLDRRGRAREGVEAI
                     SSLDEVPRRVLAR"
     gene            8648..10249
                     /locus_tag="PLB01_08510"
     CDS             8648..10249
                     /locus_tag="PLB01_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQQ77384.1"
                     /translation="MLQQLFRTKTIEQLKASVDEPGHGHLKRALSAWDLTLLGVGAII
                     GAGILSSLGTGLAGGFDSSYGVTRPAAGPALIVSFVLTAVACGFTGFCYAEMASMIPV
                     SGSAYTYAYATLGELMAWIIGWDLLLEYAISNVAIAISWGSYANNLLLGMGINLPGWL
                     AMDSRTMLQVTADFLKAHPDASSISARLGYLAQAKAGALAGGDVFANWSTLQGAPLVG
                     GFPVGMNVLAMLITILITALCAWGIKESTRFNNFIVAVKIVLLLGVIAIGAFYVKPAN
                     YHPFAPNGLSGIQAGAAIIFFAFIGFDAVSTTAEETKNPGRDLPRGILGSLAICTVIY
                     VGVCAVVSGMLPYTAYHGVADPIAHAFSSIGMNKVAGIVSIGAVAALSGALLVFQLAQ
                     PRIFMVMARDGLLPRWFEKVGPRGTPVNATWVTGVLVLLPAGLMNIDEVVELTNIGTL
                     FAFVLVSAGVLILRVRRPDAPRKFRTPLVWFSAPAGILFCLWLALGLPRTTWERFVIW
                     LVIGLFLYFLYSVRNSRLSNAAGDR"
     gene            complement(10319..10657)
                     /locus_tag="PLB01_08515"
     CDS             complement(10319..10657)
                     /locus_tag="PLB01_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77385.1"
                     /translation="MKKTTISVLALLAAFGLATSLLATMDIQKAFKEKYPEAKAVNGK
                     CSTCHTMALPKKDGDHANNAYGNDVKAAKGKDGKYDFAAIEKKDSDGDGVSNIDEIKK
                     GTNPGDKAAK"
     gene            complement(10699..12900)
                     /locus_tag="PLB01_08520"
     CDS             complement(10699..12900)
                     /locus_tag="PLB01_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQQ77386.1"
                     /translation="MRARLAAAAALAAVIALPAGAQDAPAPPKRPKIGIAFAGGGARG
                     GAHVGVLKVLEEMRVPVDVVAGTSIGSIVAALYASGMSPDEMEKVLATTDWDDALKDD
                     QPRQDQPYRQKEDDQLYLIKAELGFNMGKLVLPKGLVAGQKLNYLLRRLTLPASNVRD
                     FDRLPIPFRCVATDIVTGAKVVLSKGDLARAMRASMAIPGFFDPVEWEGKLLTDGGTA
                     DNLPVDVVRSMGADVVIAIDISTPLKKRDEITSFLSIAGQTSGFLTRLNVEQQIATLK
                     KDRDVLVVPDLEMVDTLDFKQFPKASSQGLLAANAMREKFAPYAVSEAEYAAFLKKQR
                     VPRTDPVIQEVRVEPPPGVDPRLIAGRVDTKPGTVSWPILERDLARLYELGDYETVDF
                     RIETEGGRSVLVLGGAPKPPAPTRVRLGLVLNTDFSTSSFGLRLGAYFTRLNALRGEL
                     RTKIEVGRQNSVLLEFYQPTDFHGRFFVSPTVSFTRLPFDLYLDNAVIARFRTDEFGG
                     GLDVGVSLGRYGEVRLGAVRAQTNFKTEVFTGQPVSGTAQTAALRLRAVLDQADSVTF
                     PRHGWSAGLEVFEAFDSAGGDDRYGKLLAGAGFAKTFHESTVVLSGRTDLRIGPDLRP
                     LYDQASLGGFLNLSGLAPGQLYGDNAVLARLIAFQRLAHMNSLLGTGVYAGVSIETGN
                     AWNSGLTLSDLRLAGSAFLSADTAIGPLYVAFGLADRGYHSFYLALGVPLK"
     gene            complement(12905..13267)
                     /gene="mscL"
                     /locus_tag="PLB01_08525"
     CDS             complement(12905..13267)
                     /gene="mscL"
                     /locus_tag="PLB01_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQQ77387.1"
                     /translation="MLTGFKNFIARGNVIDLAVGVIIGAAFGAVVDSLVKDLITPIIG
                     MVGGTPDFSAIKVGAVQLGAFLNALIAFLIKAAGLYFLIVLPFNKFGAKMAAATAGPP
                     PQEALLTEIRDLLKQRKP"
     gene            complement(13297..>14483)
                     /locus_tag="PLB01_08530"
     CDS             complement(13297..>14483)
                     /locus_tag="PLB01_08530"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HQQ77388.1"
                     /translation="ERERREMKRRFLRRVCLLRRTAGVAAFAVALPLALFTFREISAS
                     DVRVEGTKRFKPLDPNQKISFPRDHGAHEDSRVEWWYVTGQLETASGRRLGYQLTFFR
                     TGVTDDPAPRASSFAAKNVHLAHFARTDVSKGTFRFAERLHRDGPGAAYARTGRLSVA
                     NEDWRLEDVGGRLVLFARDGGDELSLVLSPEKPPVLHGPNGISKKGPEPEAVSTYVSL
                     TRLASAGWWTSGGTSEAVTGLSWFDHEWGSGSIGRETQGWDWFAVHLLDGRDLMLYRM
                     RAAGGAATNFSSGSLVEKDGRVLPLSSQDFSIEEVARWKSPRSGATYPARWTLRVPRA
                     GIEIEVVPVLADQELATEKSTRVTYWEGACDVTTPGGASAGRAYVEMTGYAGAGGLGL
                     FR"
BASE COUNT         2173 a         4859 c         5151 g         2300 t
ORIGIN      
        1 cctcgtccgg cggccgaagc ccgcgcgcgg agggcgggcg tgaggcggca cgaattcgtc
       61 gcggcggccc tcggggccgc ggcgctgctc cttctcgcgg ccgcggcgtt cgcggcgggg
      121 cgcccgtcgc tcgagtgcta cttccagacg acgctgcacg atcccgtcta ccagaagaag
      181 acgtacgaga aggtcgccgc gaagtggaag acgccggcgg cttcggaagt tcccgccgtg
      241 ggcaagaaga cggtcgtcca ggccgtgatc gcgaaggacg gcgcgctcgc gacgacggag
      301 gtttcaatgt cgtcgggcaa gaaagggtgg gacgcggccg cgctcaaggc cgtgaaggcg
      361 gccgcgccct tcgacccgct gcccgccggg ttcgcctacc cgaccgtgca ggtccacttc
      421 cacgtttcgg tcgtgccgta aggcgcgcta ggaactcgtc cccgagacga cgccgaactc
      481 cctgccgacg gcgacgaagg cgtccacggc ctcctgcagg tcgtcgtccg tgtggacggc
      541 cgagatctgg cagcgcaggc gcgcgtggcc ctgcggcacg acggggaacc cgaaccccga
      601 gacgtaaacg cccttctcga agagcgcatt gctcatcgcg agcgccttcg ccgtgtcgcc
      661 gacgatgacg gggacgatcg ggtggatgcc ctcggggatc ctgaagccgg ccgccttcat
      721 cttcgcgcgg aacgtcctgg cgttcttctg gaccctgagc gccggggcgg ggtcctccat
      781 gagcatcttg aacgcctcga gcgatccggc cgcgaccgcc ggagggagcg cgttcgagaa
      841 cagcgacgtc cgcgagcgct ggcgcaggag gtcgacgacg ggcgccgggc cggtcgtgaa
      901 gccgccggcc gccgcgccca tcgccttgcc cagcgtggac gtgaagatct tcaccttctc
      961 catgacgccg agctcctcgg ccgagcctcg cccggtcttc ccgaggacgc cggtcgcgtg
     1021 cgagtcgtcg acgaccatcg tcgcgtcgta tcgttcgcag aggtccgcga acgccccgag
     1081 cggcccgatc tcgccctcca tcgagaagac gccgtccgtg atgaagaggc ggcggcgctg
     1141 ggcctgggat tccttgagcg ccgtttcgag ggcggcgagg tcgccgtgcg ggacgaggta
     1201 ccgtttcgcc ttgcacaggc ggatgccgtc gatgatcgag gcgtggttca gcgcgtccga
     1261 caggagcgcg tcctcctcgc cgaggagcgg cgcgaacaga ccctcgttcg cgttccagca
     1321 ggacatgtag aggatcgtgt cctccgtccc gaagaacttc gagatcgcgg cctcgaggtc
     1381 cttgtggagc tgctgcgttc cgcagatgaa ccgcacggag ccgagtccgt tcccccagcg
     1441 gcccagcgcg tttcgcgcgg cgtcgacgac gcggggatgg ttcgcgaagc cgaggtaatt
     1501 gttggacgtg agcatgagga cgctgcgccc gtccacgttc acgcgggcgc cggtcgggcc
     1561 ctcgaggacg cgctctttct tccaggtctt cgcggccttc agagactcga tctcggcgcg
     1621 gcagtaatcg atgaggctcg tcatccggcg agcctaaccg aaaaaagatc ggcgggcctc
     1681 gtggcccgcc gtggaggaag gaacgggtgc ggtccccgcg gttacttctt cgtgatctcc
     1741 gtcgcgagga tctgcggcga cgccgtgggc gcgaagacgg ccatcgggag cgtcacgccc
     1801 ttgacgtgca cggacgagcc gacgccgatc ccgctcgcga ccctggtggc cggcgtgacg
     1861 aggacgtcct tgccggagat cttccagtgg ccgagggcgg cgcccgcgga gttcgcgagg
     1921 cccggcggca tcgattccac gacgccgtcg aactccaccg agcggtccgt cggcgggggc
     1981 ggcgtcgaga tgaccttgac gatcgagatc gcgacgggag gcatcgacgg catccccatg
     2041 cccggcggga cgatcgtgac cttcgcgaga acgtccacga cgtcgcccac cttcgggtcg
     2101 ccgacgatct tcgtgtcggc gttcacctgc acgaacttgc cgtcgaccgt ccaggcggac
     2161 gcgtcgatct tctcgacctt gccgcggaac gcctcgatgc gcgtcgtcgg gatcgtgtac
     2221 gcgtgcacgc tcttcgcggt gacgccgctc gcgtcggtcg aaacgacggt gacggcctgc
     2281 atgccgatgg cgagatccga gaggcccttg acgggtgagc cgtcggcctt cgagccggac
     2341 cactcggtcc ccgcgttcgt cttcacgctc gtgtagagga gcgtgaacgt cccctgtgcc
     2401 gcgccgacac cctgcacggt tccggacagg ctcccggagt tcgtgaggaa gacgacgacg
     2461 ctcgtcgcgg ggagtcgcgg cgggaagacc gcggggatcg cgtcgggcac ggtcacgtgg
     2521 gcgacgatgc gcgagccgac gagaatgccg gcccacggca acggactcgc caggcggtcc
     2581 tcgccgcccg tgatcctcgc gttcgtgacg tcgatctgaa gcgcgccgcc gaggacgtcg
     2641 aggatcggcc cgttcacctt caagacgacc gagtcgaccg tcgaagcggg cgcggcgatg
     2701 ggaatcatcg ccgtgcccat cgttccctgc gcgagcgcgg gcatcgcgaa gacggccggg
     2761 accgagagga cgagagccac gacggccagg gcggtgggtt tggagcggat gttggggcag
     2821 gccgttcgag gcgggaggga aacgaacgtc atggctcctc ctgtcggacg ttccggagtg
     2881 acgcccggag gagccatctc acgcgacgcg accgagggtc agcgccgccg gcctggtttc
     2941 tttttcggcg ccggcttctt cctggtggcc ttcttcgccg ccttcttcgc gggcgccttt
     3001 ttgacctttt tggcggccgc ctttttcgcg ggcttcctcg atgccgcctt cttcgcgggc
     3061 ttcttgccgg tcccgcgcgc ctgctcacgc ttcttgcgcg cgttgagctt ctgcttcacg
     3121 agctcgtcga cgcccgaagc cggcacccac tcctcgtccg ggtggagctc ggaggcctgg
     3181 aacaggatga tccggtagcc gtccggatcg aggatctcgg ccgagcggcc cgtgaactcg
     3241 tccgccgggg gccgcgtgac cgtgacgccg cgctccttga ggcgggctat cgcgtcgtcg
     3301 aggctggcgg tcgagaacac ggcggcgacc tggttgcgcg cggggacttc cgacgggcgg
     3361 agcggggtga gcttcaggag aagcggaccg agggcgaact cgacccagtc gttctcgaac
     3421 ccgcccgcct ccgggagccc cagcgtttca ccgtaaaaac ggcgcgaccg cgaaaagtcg
     3481 ctcgcaaaaa catggattcg cctgagggcg aggaacatct tcatcctccg aaacggggcg
     3541 ccggaggata acaggaagtc ccttactttt caatgggttt acgtggaatc cggattcccg
     3601 tagggggcat gggaaggggc cccgcagggc tcgacccacg aacttccgtc gggtccgaac
     3661 tccttcttcc agatcgggac gcgggccttg atggcctcga ttccggcccg gcaggctgcg
     3721 aaagcctcgt cccggtgggg cgcggccgcg gcgacggcga cggcgacatc cccgatgtcg
     3781 aggctgccga tccggtggcg aaaggcgacc cgcgcccccg gatgggcctc ctcgatctcc
     3841 gtcgcgatgc ggtccatctc cttctccgcc atcgggatat aggccgagta ctccagccgg
     3901 gtcacgcgtt tcccgtcgtt gtggtcgcgc acgaccccca cgaagagcgc gaggccgccg
     3961 tctccgtcgc gccggacggc ggcgaggagg ccggcgggtt cgacggggga ctcggtcagg
     4021 tgggacatcg agtcatgata agacccgccg ccatgacccc gcggcgccgg cgaatcctgg
     4081 ccgtctcggc actcaagggc ggcgtgggca agaccacgac ggccgtgaac ctcgccgccg
     4141 cggccgcgcg cgccggggtg aggaccctcc tcgtggacgt ggacccgcag ggcagcgtcg
     4201 ccacgtcgct cggtctcccg ccgtccgccg gcctctcgaa gtggctcctc aagaaggtgg
     4261 acttcgagga agccgtcgtg aggggctcgc ggccgaacct cgacgtcctg cccgccggcg
     4321 aggacctcct cggcgccgag gacgcgctcc gcgagaaggg gaaagagaag cgcgtgggcc
     4381 ggctcgcgcg gcgtttcgcg tcgctcgagg acgaaggata cgaggcgatc gtcctcgact
     4441 gcccgcccgc ggcggggctg ctgctcgaga acgtgtacca cgcggcggac gagatcctgc
     4501 tgcccgcgaa gctcgacttc ctctcgctgc ccgcgctgga gaagacgcgc gtgttcgtga
     4561 agcacgcgaa cgagacgcgc gagaaagcgc tgaagatcga cggcgtcctc ccgaccttct
     4621 acgacctgcg caccgccgtc tcctcggagc tcctcgacac gctccgcgag cgcttctcga
     4681 agatcctcac gccgatccgc atcaacgtgg cgctcgccga ggcgccgtcg gtcgggaaga
     4741 cgatcttcga gttcgacgga tcggcgcgcg gcgcggtgga ctacgcgctc ctcgcggaag
     4801 aactgaagct ggactagccg cccgcgacgg gcggcaggag cgcgaccgtg tcgccgttct
     4861 tcacggcgtc ggtctcgcgc gcccaggtct cgttcacggc ggccttcgcc ctgccgccga
     4921 aacgcgcgat cgccgggtag gcggcttcga gggcctttcg cagcgactcg acggtcgcgg
     4981 gctcggcgac ttcgaccgac acctgcggcc cgagctcgtc cctgagcgac gcgaagagga
     5041 ggacgtcgac ccgcacggac cgattctagt cggcgggcgg acgcgtcagc ggaagtagcc
     5101 gttgacgtcg acgatgacgt cgacgtttcc cccggacgaa ttgaagaccg tgatggttcc
     5161 ggaaggggtt cctccgattg agacaatggc gttgttcgcg cgtgtttggc ccgcggagaa
     5221 attcaagacg ctggtcgcgg ggacggcgag cccggccggg aacacgccga ggaatccggg
     5281 gctctccgtt cccgtcaccg tgacgttggc ggagacgctg atggcgtccg taggaatacc
     5341 gcagagcccg aagagagtga aatcccgaag cgaattcggt ggcaaagccc cgcccggcgt
     5401 tcgcgtgtcg atcgcccggc agggcgggag aaggctcagg cgcgtcgcgc cgccaaacga
     5461 cgcaaccctg aagaggaagg cgtccggcag gccgttggcg tccgaggcga cgagttccga
     5521 ggcgtcgctg ttgaacgcga cgaggttccc gtccgcgctc aaccgagggc cgctcgaacc
     5581 aagactgccc gtcgccgcct ggtcggcgaa cttacggctg acgagcatcg tcctcgaggt
     5641 tcggcggtcg tggaggaaga cgtcgtaggt gtcgttccca tccacttggg acgggacgag
     5701 gtccgtcgcc cgacttatga aggcaacccg gcttccgtcg gcgctgatct ccggattgcg
     5761 cgattcgaag ctgcccgtct gtgtcgcgcc caccgccttg tggctcacga gcgtgttggt
     5821 cccggtcagc cgatcccaca cgaagacgtc gttcgagagg ccgggcgagt cctgctgccc
     5881 cgggacgaga gaggtggcgg cgctgcagta ggcgacggcg cgtccatccg cgctgatacg
     5941 cgggtcgaat gagccggagt ccgcggtcgt gagcgggagg ccgggcgcgt gactgacgag
     6001 gacggaaacc cctgcaagcc tgtcgaagca gtagacgttg ggccagccgg ggatcgtgtc
     6061 ggtgaggccg gcgacgagat cgctcgccac gctgaaaaac gcaatgaacc ggccgtcggc
     6121 gctgatgctc ggtgcgaatg actggttgtt gccggccgtc gcggacgttc cgacgcgccg
     6181 ggtgacgaga gagatcgtcc ccgcctcacg gtcgaacagg aagacgtccg acgtgccctg
     6241 ccagtcgatc tggcctggca cgaggtcgcc cgcgctgcta gcgaacgaga cgaaccggcc
     6301 gtcggcgctc atggcgtgcc attgtgttga tccgtcgccg gttctggagg gaaacccggc
     6361 tcggtggctc acgagcgtca gggacgtggt gacccggtcg aacaggaaga gatccgctcc
     6421 gggcggcggg tggcccacca cttctccggg gacgaggtcc gttgctctgc tcaggaacga
     6481 cacccatcgt ccgtccgcgc tgatcgccgg cgcggacgag tcggcgtttc ccccggcgcc
     6541 ggggagcccg gtcgcgtgac tcacgaggaa attcgctccg gtggaccggt cgaaaaggac
     6601 cacgttgtag gtatagccgt tccccgtttg tcccgtgacg aggtcggttg cggcgctcag
     6661 gtaggcgatc cagcgcccgt cctcgctgat caccggagaa tccgaggggc cgttggcggt
     6721 cgacgagaag gaggacgcgg aatggctgac aatttgcacg gtgtcgttcg agagatccgt
     6781 gacgaacacc gcgccgaaca gattgccggc ctgactcgga accaggtcgt tcgcggtgct
     6841 cgtgaacgtc agactgcgcg cgtctcccga gaaacacgag ggcgtcgggc ccgagaaagg
     6901 gtcgttcgtc aggaagctcc cgccggcggc ggtcgtcgtg gcgggcgtgc ctgcaggatg
     6961 cgcgacggcc agcacttcgc tcgcggtcgc gctcgacgcg ccgacgagaa ggcacaggag
     7021 aagcgggaac gtgcggttag acatggcaac tcgacatcat aggagccccg catcccggag
     7081 aaccgatctc acgccctaga atcgcccacc tcggaggtcg cgcatgtctc agggacgtct
     7141 cgccgggaag aacgcgctcg tcacgggcgc gaacaagggc atcgggaggg ccatcgcgct
     7201 gcggctcgcg gccgagggcg cgcgcgtggc cgtcgcggcc cgcaaggaag ccgaggccga
     7261 ggccgtcgcc gcggagatcg tgaaggcggg cggagaggct cacgccgtcg ccctcgacgt
     7321 gacggacgct gcgtcggtcg cgaccggcgt cgaacgggcc gcgcggccgg atcggcggat
     7381 cgacgtcctc gtgaacaacg cggggctcgg cggcccctcg ccgctggacg gcgacgtgcg
     7441 ctcggacgag gcctggcacg cgatcctcga ggtgaatctc acgggcgtct ggcgcgtcct
     7501 gcgcgccgcg aacccgttcc ttgcgcccgg cgggcgcgtc gtcaacctct cctccgtgac
     7561 cggacgattc ggcgtcgcgg ggatggcggc ctattcgacg acgaagcacg ggatcatcgg
     7621 gctcacgcgc acgctcgctc tcgagctcgc gccgcggaag atcacggtga acgcgatctg
     7681 cccgggctgg gtcgatacgg agatgggccg ctcgggcatc gcacgcatgg gcaaggcgct
     7741 cgggaagagc cccgacgagg cgttcgcgtt cgcgggaaag atggcgccgc tcggcgaggt
     7801 cctcacgccg gacgagatcg gcggcctcgt cgtctacctg gcgtccgacg acgcgcggaa
     7861 cgtcacgggc caggccctcg tgatcgatgg cggccaggtg atgccctgag gcccatccgg
     7921 gccgtcttct tcgacgcggg cgacacgctg ctgagggccg acccgccggt cgaggaggtc
     7981 taccggggag ccttcgcgcg ctacggcgtg cgggcccagg ccgaagaggt acacgccgcc
     8041 gtccacgcca cgtggcgcga cgtggacctc gcgcgcgagc gcggcgaaga gcgctggggc
     8101 ggagccggcg gcgaggcggg cttctggcgg cgtttcgtcg ggaccgtgtt cacccgagtc
     8161 ggcggtgggg cgatgcccga gcctctcctc ggcgagctcg tcgcgcattt cggcgccgag
     8221 cggcactggg ccgtgtaccc cgagacgcgc ggcgtcctcg cggcgctgcg ggcggcgggc
     8281 ctgcgcctcg tgatcgtctc gaactgggac tcgacgctcc cgtcgctgct cgagcgcctc
     8341 ggcctcgcgg ccgcggtgga cgacgtcgtc gtctcggccc tcctcggcgt gagcaagccc
     8401 gcgcgcggga tcttcgacga ggccgtgcgc cgcgcgggcg tcacgcacgc cgaggcgctg
     8461 cacgtcggag actcactcac ggacgactac cacggcgccc gggacgcggg cctctcgtcc
     8521 ctcctcctcg accggcgcgg gcgcgcgcgg gagggcgtcg aggcgatttc ttcgctcgac
     8581 gaggtcccgc gccgcgtcct cgcccgttag actcctgcga ccgcgtcgcg aggaagaggg
     8641 agggtccgtg cttcaacagc tgttccggac gaagaccatc gagcagctca aggcgagcgt
     8701 ggacgagccc ggccacgggc acctcaagcg cgcgctctcc gcctgggacc tcacgctcct
     8761 cggcgtcggc gcgatcatcg gcgccgggat cctctcgagc ctcgggacgg gcctcgccgg
     8821 cggcttcgac tcgtcctacg gcgtcacgcg gcctgccgcg ggcccggcgc tcatcgtgag
     8881 cttcgtcctc acggccgtcg cgtgcggctt cacgggcttc tgctatgcgg agatggcctc
     8941 gatgatcccc gtctcgggtt cggcgtacac gtacgcgtac gcgacgctcg gcgagctcat
     9001 ggcgtggatc atcggctggg acctcctcct cgagtacgcg atctcgaacg tcgcgatcgc
     9061 gatctcgtgg ggcagctacg cgaacaacct tctcctcggg atgggaatca acctgccggg
     9121 ctggctcgcg atggacagcc ggacgatgct gcaggtcacg gccgacttcc tgaaggctca
     9181 cccggacgcc tccagcatct ccgcacgcct cgggtacctc gcgcaggcga aggcgggcgc
     9241 gctcgcgggc ggcgacgtct tcgcgaactg gtcgacgctc cagggcgcgc cgctcgtggg
     9301 cggcttcccc gtggggatga acgtcctcgc gatgctcatc acgatcctca tcacggcgct
     9361 ctgcgcgtgg ggcatcaagg agtcgacgcg cttcaacaac ttcatcgtcg ccgtgaagat
     9421 cgtcctcctc ctcggcgtca tcgcgatcgg ggccttctac gtgaagcccg cgaactacca
     9481 tccgttcgcg ccgaacggcc tctccggcat ccaggcgggc gccgcgatca tcttcttcgc
     9541 cttcatcggc ttcgacgccg tctcgacgac ggcggaggag acgaagaacc cggggcggga
     9601 cctgccgcgc ggaatcctcg ggagcctcgc gatctgcacc gtgatctacg tcggcgtctg
     9661 cgccgtcgtc tccgggatgc tcccgtacac ggcgtaccac ggcgtcgccg acccgatcgc
     9721 ccacgcgttc tcgtcgatcg ggatgaacaa ggtcgccggg atcgtctcga tcggcgccgt
     9781 cgcggcgctg tcgggcgcgc tcctcgtgtt ccagctcgcg cagccgcgca tcttcatggt
     9841 catggcgcgc gacggcctgc tcccgcgctg gttcgagaag gtcgggccgc gcggcacgcc
     9901 ggtgaacgcg acgtgggtga cgggcgtcct cgtcctcctg ccggcgggcc tcatgaacat
     9961 cgacgaggtc gtcgagctca cgaacatcgg gacgctcttc gcgttcgtcc tcgtgagcgc
    10021 cggcgttctc atcctgcgcg tgaggcgccc ggacgcaccg cggaagttcc gcacgccgct
    10081 cgtctggttc agcgcgcccg ccgggatcct gttctgcctc tggctcgccc tgggcctgcc
    10141 gcgcacgacg tgggagcggt tcgtgatctg gctcgtgatc gggctcttcc tgtacttcct
    10201 gtacagcgtc cgcaacagcc ggctctcgaa cgccgccggc gaccgctgag gcgcccgcaa
    10261 acgatgagcg cccggggccg cgaggcaccg ggcgcaccgt tcgctcgcgg gagagggtct
    10321 acttcgcggc cttgtcgccg gggttcgtcc ccttcttgat ctcgtcgatg ttggacacgc
    10381 cgtcgccgtc cgagtccttc ttctcgatcg cggcgaagtc gtacttgccg tccttcccct
    10441 tggccgcctt gacgtcgttg ccgtacgcgt tgttcgcgtg gtcgccgtcc ttcttgggga
    10501 gggccatcgt gtggcacgtc gagcacttgc cgttgacggc cttggcctcc gggtactttt
    10561 ccttgaaggc cttctggatg tccatggtgg cgagcaggct cgtcgcgaga ccgaacgcgg
    10621 cgagaagggc gagaacggag atcgtcgtct ttttcaaagg gtcctccttg aggatcggta
    10681 tttaaccaga gaaccggatt acttgagcgg cacgcccagc gcgaggtaga agctgtggta
    10741 gccgcggtcg gcgaggccga acgccacgta gagcggcccg atcgcggtat cggccgagag
    10801 gaaggcggag ccggcgagcc gcaggtcgga gagggtgagg ccggagttcc aggcgttgcc
    10861 ggtctcgatc gagactccgg cgtagacccc cgtcccgagg agcgaattca tgtgtgcgag
    10921 acgctggaac gcgatgagcc gggcgagaac cgcgttgtcg ccgtagagct ggccgggcgc
    10981 caggcccgag aggttcagga agccgccgag cgatgcctgg tcgtaaaggg gccggaggtc
    11041 cggcccgatc cggaggtccg tccggccgct caggacgacg gtggactcgt ggaacgtctt
    11101 tgcgaagccg gcccccgcca acagctttcc gtaccggtcg tcgccgccgg cgctgtcgaa
    11161 ggcctcgaaa acctcgagac cggcgctcca gccgtggcgt ggaaacgtca cgctgtcggc
    11221 ctggtcgagg acggcgcgca gccggagggc ggccgtctgc gccgttccgg aaacgggctg
    11281 gcccgtgaag acctccgtct tgaaattcgt ctgcgcgcga acggctccga ggcggacctc
    11341 gccgtaccgg ccgagggaca cgccgacgtc gagccccccg ccgaactcgt cggtccggaa
    11401 ccgcgcgatg acggcgttgt ccaggtagag gtcgaacggc aggcgcgtga aggagaccgt
    11461 cggagacacg aagaaccgcc cgtggaagtc cgtcggctgg tagaactcga ggaggacgct
    11521 gttctgccgg ccgacctcga tcttcgtccg gagctcgccg cggagcgcgt tcagccgcgt
    11581 gaagtaggcg ccgagccgga ggccgaacga gctggtcgag aagtccgtgt tgaggacgag
    11641 gccgagccgg acgcgcgtcg gggcgggagg cttcggcgcg ccgccgagga cgaggacgct
    11701 cctgccgccc tcggtttcga tgcggaagtc gacggtctcg tagtcgccga gctcgtagag
    11761 gcgggcgagg tcgcgctcca ggatcggcca gctgacggtg cccggcttcg tgtccacgcg
    11821 gcccgcgatg aggcgcggat ccacgccggg aggaggctcg acgcgcacct cctggatgac
    11881 cgggtccgtg cgcgggacgc gctgcttctt caggaacgcc gcgtactcgg cctcggagac
    11941 cgcgtacggc gcgaacttct cgcgcatcgc gttcgcggcg aggaggccct gagaggaggc
    12001 cttcgggaac tgcttgaagt ccagcgtgtc gaccatctcg aggtcgggga ccacgaggac
    12061 gtcgcggtcc ttcttcaggg tcgcgatctg ctgctcgacg ttgaggcggg tcaggaaccc
    12121 cgaggtctgg cccgcgatcg agaggaagct cgtgatctcg tcgcgcttct tgagcggcgt
    12181 cgagatgtcg atcgcgatca cgacgtccgc tcccatcgaa cgcacgacgt cgacgggcag
    12241 gttgtccgcg gtgccgccgt ccgtgaggag cttcccctcc cactcgaccg ggtcgaagaa
    12301 tcccgggatc gccatgctcg cgcgcatcgc gcgggcgagg tcgcccttcg agagaacgac
    12361 ctttgccccc gtgacgatgt ccgtcgcgac gcagcggaac gggatcggca gccggtcgaa
    12421 atcccgcacg ttcgaggccg ggagcgtgag ccggcggagg agatagttga gcttctggcc
    12481 ggccacgagg cccttcggga ggacgagctt gcccatgttg aaccccagct cggccttgat
    12541 gaggtagagc tggtcgtctt ccttctgtcg gtagggctgg tcctggcgcg gctggtcgtc
    12601 cttgagggcg tcgtcccagt ccgtcgtcgc gaggaccttc tccatctcgt ccggcgacat
    12661 gccggaggcg tagagggcgg ccacgatcga cccgatgctc gtgcccgcga cgacgtccac
    12721 gggcacacgc atctcctcga ggaccttcag gacgccgacg tgcgcgccgc cgcgtgcgcc
    12781 tccgcccgcg aaggcgatgc cgatcttcgg gcgcttcgga ggcgcgggag cgtcctgggc
    12841 gcccgcgggc agggcgatca cggccgcgag tgcggcggcc gcggcaagtc gggctctcac
    12901 gggatcacgg cttccgctgc ttgagcaggt cgcggatctc ggtcaggagc gcctcctgcg
    12961 gaggaggccc ggccgtggcg gcggccatct tcgcgccgaa cttgttgaac ggcaggacga
    13021 tgaggaagta gaggccggcc gccttgatga ggaacgcaat gagcgcgttc aggaaggcgc
    13081 cgagctggac ggcgccgacc ttgatcgcgg aaaagtccgg cgttccgccg accattccga
    13141 tgatcggcgt gatgaggtcc ttcacgagcg agtcgacgac ggcgccgaac gcggctccga
    13201 tgatgacgcc gaccgcgagg tcgatcacgt tgccgcgggc gatgaagttc ttgaagccgg
    13261 tcagcatgag tcctcctgaa gtttctggag ccgagtttag cgaaagagac cgaggcctcc
    13321 ggcgccggcg tagcccgtca tctcgacata cgcgcggccg gccgacgcgc cgccgggagt
    13381 cgtcacgtcg cacgcgccct cccagtacgt gacgcgcgtc gacttctccg tggcgagctc
    13441 ctggtccgcg aggacgggca cgacctcgat ttcgattccc gctctcggaa cgcgcagggt
    13501 ccagcgcgcg ggatacgtgg cgccgcttct cgggctcttc caacgggcga cctcttcgat
    13561 ggagaagtcc tgcgaggaga gagggagcac gcgcccgtcc ttttcgacga ggctgcccga
    13621 gctgaagttc gtcgcggctc cccccgccgc gcgcatgcga tagagcatga ggtcgcgccc
    13681 gtcgaggagg tggacggcga accagtccca gccttgcgtc tccctgccga tggagcccga
    13741 gccccactcg tggtcgaacc aggaaagccc ggtgacggcc tcgctcgtcc cgccggaggt
    13801 ccaccagccc gcgctcgcga gccgcgtgag gctgacgtac gtgctgacgg cctcgggctc
    13861 gggtcccttc ttcgagatcc cgttcgggcc gtgcagaacc ggcggcttct cgggcgagag
    13921 cacgagggag agctcgtccc cgccgtcccg cgcgaagaga acgaggcgcc cgccgacgtc
    13981 ctcgaggcgc cagtcctcgt tcgcgacgga caggcgcccc gtccgcgcgt acgcggcgcc
    14041 ggggccgtcg cggtgcaggc gttcggcgaa acggaacgtc cccttcgaga cgtcggtcct
    14101 cgcgaagtgc gcgaggtgga cgttcttcgc cgcgaaggac gacgcgcgcg gcgcggggtc
    14161 gtccgtgact cccgtgcgga agaacgtgag ctggtagccg aggcgcctcc ccgaggccgt
    14221 ctcgagctgg cccgtcacgt accaccactc gacgcgggag tcctcgtgcg cgccgtggtc
    14281 gcgggggaag gagatcttct ggttcggatc gagaggcttg aagcgcttcg tgccttcgac
    14341 gcgcacgtcg gaagcagaga tttctctgaa ggtgaagagg gcgagcggca gcgccacggc
    14401 aaacgcagcg acgcccgcgg tgcgccgcag caaacacacc cttctgagaa atcttctctt
    14461 catctctctt ctttctctct cat
//
LOCUS       DAOMFY010000081        14401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1285_length_14401_cov_8.110484, whole genome shotgun sequence.
ACCESSION   DAOMFY010000081 DAOMFY010000000
VERSION     DAOMFY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14401
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1285_length_14401_cov_8.110484"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PLB01_08535"
     CDS             <1..905
                     /locus_tag="PLB01_08535"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ77389.1"
                     /translation="SPLPRIAFVGLGTMGRPMAANLARAGFPLSLATRTPGKAATIAA
                     ELSGGGRDVRAAVTPADAARGASVIVSCVPDAPEVEEVHLGPGGTAETAARDTVVIDC
                     STIDAANARTLAAALAAKGIAFLDAPVSGGQKGAVEGTLTFFVGGDPAALDGARPVFE
                     AMGKRITHLGPSGAGQLGKAANQILVANNLMAVSEALAFAAKAGLPLGPLHEALAGGA
                     ARSWALEVLGAKMIDRDFKPAFAVKHQQKDLAIVLRNARAAGVPLPGVALVHQLLASL
                     EAEGRGEDGTQALLTVYEKLAAGR"
     gene            930..1388
                     /locus_tag="PLB01_08540"
     CDS             930..1388
                     /locus_tag="PLB01_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77390.1"
                     /translation="MNRLRLTALVAVLVASSAALAETQKFERSYPFVGDKDVKVGIKV
                     GDATIESFRIRHWPDSDDFEKARKDLNDTHTMVVEFTYSNRDRDKDYKCKYVVTIPAG
                     KDGKPFGENDRTATLDKGKIDDTNSMFVKMRTNDYKVAKTIKISFEIWRK"
     gene            complement(1398..1637)
                     /locus_tag="PLB01_08545"
     CDS             complement(1398..1637)
                     /locus_tag="PLB01_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77391.1"
                     /translation="MESAAELHEARVLRAQIEILEGELQAAGAGDRVLLAEQVARLKR
                     DYRRVLARQADEDYRRRREAWRAEQDARPRARACA"
     gene            1816..2391
                     /locus_tag="PLB01_08550"
     CDS             1816..2391
                     /locus_tag="PLB01_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HQQ77392.1"
                     /translation="MATEEPKYRIERDYGSFEVRVYEPTVVAETVVTGSFGDGSNEGF
                     RRLAGYIFGGNDGGRKIAMTAPVGAEPAPRSKGTSIAMTAPVGSERTAEGWVVSFTMP
                     SSFTLKTLPAPDDARVRLREVPGRRVAAVRFSGTWGAEKFEALAAKLESDAKGTGLSP
                     ADAPPVFARYDPPWTPWFMRRNEVLIPLEGR"
     gene            complement(2406..3464)
                     /gene="arsS"
                     /locus_tag="PLB01_08555"
     CDS             complement(2406..3464)
                     /gene="arsS"
                     /locus_tag="PLB01_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HQQ77393.1"
                     /translation="MTLSLHARGAPLAAPEKQVAAVESVDLSASAHGGDFARALAASG
                     RDALRPGPVEIFQINLGKLCNMTCRHCHVDAGPDRTDEMMDRATAEACLAALDRTGAH
                     TVDLTGGAPELNPNFRYVVDEAAARGKHVIDRCNLTILLVSGFRDLPAWLGERGVEIV
                     CSLPHWRKRGTDAQRGDGTYEKSLQALHLLNEAGYGSGDPRRTLTLVSNPVGAFLPAN
                     QRAAEREWKEGLLREHGVAFDRLIFLNNMPISRYLEWLESSGNLASYLELLVASFNPA
                     TIDGLMCRNTLSIGWDGRIYDCDFNQMLDLPARGSNGAFHVRDLDVAALAARTVVTRR
                     HCFGCTAGAGSSCGGALE"
     gene            complement(3461..3793)
                     /locus_tag="PLB01_08560"
     CDS             complement(3461..3793)
                     /locus_tag="PLB01_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017712460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HQQ77394.1"
                     /translation="MDGYYKSEHLAHFASIAEGNKELADKFFAWYGAVFADGALSARE
                     KALIALAVAHAVQCPYCIDAYTQESLKNGADLEQMTEALHVTAAIRGGASLVHGMQML
                     EHAKKASM"
     gene            complement(3821..4738)
                     /locus_tag="PLB01_08565"
     CDS             complement(3821..4738)
                     /locus_tag="PLB01_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ77395.1"
                     /translation="MEDFERRRYFETLATTPQTLKAALKGLSKKVLLWTPAPGKWSIL
                     EILCHMRDMEREAYIERYTRILTENEPSLPNLDGEAIAILRGYRSQKAPEVVREWVAL
                     RRQTLQLLRKAGKAQWARAGIHATAGRLTMEDLIQRHAVGNDEAHLRQIEAIKRRHAI
                     LERLAASPAALAAALKGAPDDVLRRKAAGGKWSMLENAAHLRDIERVYQGRFSQMAFG
                     DKPSFWMLDNDRAAETLKYAEADPAEVAKEFKRLREDTVSLLRALPHEGWLKTGVHPE
                     AGEITVEQLAVRLANHDDGHLGKIKTLRA"
     gene            complement(4748..5344)
                     /locus_tag="PLB01_08570"
     CDS             complement(4748..5344)
                     /locus_tag="PLB01_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ77396.1"
                     /translation="MKVAFIGTHGVGKTTLCYDLAAALKKRDLTVELVREVARECPLP
                     INRDTTLKAQSWILHTQIAWELQAEAKADVVFCDRAVLDNFCYMKRAFTGAPEEAVLE
                     GLVRSWTRSYDALFKVPIVGDPRFDGVRDTDLAFQHDIDRSIQQLLAVWGVPHVVLDP
                     ARRADWSQNVLDTLLPRLKPQELLFPELDKPLNGVRAG"
     gene            5490..6053
                     /locus_tag="PLB01_08575"
     CDS             5490..6053
                     /locus_tag="PLB01_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77397.1"
                     /translation="MKTRHPFLASVLAAVLLLAVSPMPAPIYMNIPNGATLALRTKVE
                     FKQAVLCDGSVRLEPGSYDVSFKSLGDGSVRTTISGNGKTGHATGKVMGHEVVVQGGL
                     QPGAAASVQKIQPGASNAAAGQGSAQANTPPSFASMGFTPQSQVSLRQEGNKLDVIVN
                     GQGSNQILIGLLLPAVQKVREAANKPH"
     gene            6069..6551
                     /locus_tag="PLB01_08580"
     CDS             6069..6551
                     /locus_tag="PLB01_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77398.1"
                     /translation="MKIRRTILASLLSAVLLLATSPLPAQTYTLTTQAPQVTTMTPFQ
                     INVYLAQPTLCGTTKVLLPKGAYAVKIESLGAGSVRATFTGNGKTCQNPGTLTASSGN
                     PLGGTTSSYGTTTAGQSFAAMGFTPQSQVVTQTVGTKLNVIVKGLGTNQILIGLTLPS
                     "
     gene            6562..7092
                     /locus_tag="PLB01_08585"
     CDS             6562..7092
                     /locus_tag="PLB01_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77399.1"
                     /translation="MRSVLFALVLSASTAASAMPTAVESGPVVAAGQTFQKGVVLETA
                     VLCDGSVRLEKGTYDVKFESLGKNKIRATFFQGGARKGEANGIIIIGGSNQALGGVRG
                     PGPGPAPGGTNAFSFNDLGLHPSSAHALQKQGDKLNLVVNGQGTNQILIGLLLPAVQK
                     GGLQPPGIKPGAPSGP"
     gene            complement(7096..8256)
                     /locus_tag="PLB01_08590"
     CDS             complement(7096..8256)
                     /locus_tag="PLB01_08590"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQQ77400.1"
                     /translation="MRRPPLDGVVVLDLSRVLAGPLATMLLADLGARVVKVEDPRGGD
                     VSRGWKPPALDGEAAYYLSVNRRKESAVVDLDTPEGQEFVRRWAAKADVLVENFLPGA
                     LEKRGIGVDALRARSPHLVACSISGAGDAGPEAGAPGFDLLAQGATGLMSITGPAGGA
                     PHKMGVALVDVLAGWAALAEILAALHARERDGTGAHVKTNLVSTGLAALVNVAGSALV
                     TGREAARHGNAHAAIEPYRMFEAADAPFLLAVGTDRQFGFLCERVLGRPELSRDARFE
                     TNAARVVNRDELVPILAAHFSREPREAWLARCKACGIPAGPVLGVLEALHAPQARSLG
                     SVLETRRGDRSVPTVRPPFFLEGFSDPPAAAPPRLGEDTERLFAEVGLPAPR"
     gene            complement(8256..10571)
                     /locus_tag="PLB01_08595"
     CDS             complement(8256..10571)
                     /locus_tag="PLB01_08595"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQQ77401.1"
                     /translation="MSARLPRVSLFGGIVLLGAGLAAAFAVLPEWRAVPPRPVNAVES
                     GRAAMRDAGATLTRATLVFSARPNLASEFERAYRRLGADARTYLETTGGAFAWTVKGA
                     VSVPGGGAGPVEIALSPDGSVQAVDWSPEGSIFEGPDEKTRVARRAFAAKLEDLVRGG
                     RKKAGEELTFPATNVMVRVQPLEAAPGAPAENVQTLESGGIKLSVARRLRDPEAPQHF
                     NAQGLARRLYRFIPSLVVFLAVGVAFGLLLYRRRLGFRIAIFLAALALLYEVLGGVSG
                     EELAAGGFVAFAIGIRIFSLLYLLALWAVAESLLRDTVPGFSTSLDSFAARRLGPRGG
                     VAILSGLGAGAATVGITLLAFAAAALAAPRGAWPTGPSFAYPLFQTQKGPFYEGTFDA
                     AIFVLFVALLRFVLPRAWAGTAGAILFALYLSSGMPLHPWGASLALCLLFALVFRSVF
                     DAHGLAALLVAAVSSALLRDALVACRALDGNALAALATVLPLAALAVAGVVGWMRPES
                     EDEGRLDAPEYVRRLESERRVKYEMDLLSRMQLSLLPEKPPAVDGLDLAVKTVLATEA
                     GGDLFDFVVDDAGSLWIAAGDVSGHGYSCGIQQAMVMASLASLVKAGRRPSEILVEID
                     RVLRMGRSGRLFTSVALLALDPKTGKGLLANAGHPYPLLLEEGVCREITGSGLPLGQG
                     PKRTYEDIAVEIPRGGVLILASDGLYEGPDLVDTPYGYDRPRDVLTAVSLWRRPAEAI
                     VEALFADWRRHVGDGTPSDDTTVLVVKRPLF"
     gene            complement(10568..11533)
                     /gene="nadA"
                     /locus_tag="PLB01_08600"
     CDS             complement(10568..11533)
                     /gene="nadA"
                     /locus_tag="PLB01_08600"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQQ77402.1"
                     /translation="MSAIPSQDVALDIRRLKEERNAIVLAHYYQESEIQDVADFVGDS
                     LELARRGKEANEAVIAFAGVHFMAETSKILTPEKTVVVPDLEAGCSLADGCPAPLFAE
                     WLKKYPGHTVVSYINCSAGVKALSDVIVTSSSAEIILRQIPDPVVFAPDRNLAAWLEK
                     KLDRKFVVWPGTCVVHETFSEQKLLDLVTRHPGAEVIAHPECEPRILAHAAFVASTSG
                     LLKRVVTSPKQAFIVATEEGILHQMRLAAPGKTLIPAPPVADCSCNTCPHMKRNTLEK
                     LAACLTDLAPRIEVPEAVRVKAKRSIERMLEMTAGQTLAPAGARS"
     gene            11641..12645
                     /locus_tag="PLB01_08605"
     CDS             11641..12645
                     /locus_tag="PLB01_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77403.1"
                     /translation="MSQPAASPWISNPRWDLIVLSFSAVLVAVPPLVHSLGKTTAGGV
                     DLLITLLVGGPHMYATFLKTVFEPNFRKLHPALTFAPVVIIPMIVISGALFAFQALLT
                     FFFTWASMHVCDQASYIAYLYREKGGAAKPWQRGLDVSVILFSLYSYAMYRHVNGTFK
                     IGDTTLWFPEFLKHAWFADAFALVTAGLIATWIVYTLNQLRRKEVGGPYVFFMALTVG
                     VALVIPRMPELSVSFQGFNAWHSFQYLGLTYLILQQDNSIAKESLAKRLAQPGKFFRY
                     YGWNILLTTGATLIIVLLTIVLKLPAEPSYYAVVLSFLLVHYAHDHVLFAFGSGFRPK
                     "
     gene            12642..13430
                     /locus_tag="PLB01_08610"
     CDS             12642..13430
                     /locus_tag="PLB01_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ77404.1"
                     /translation="MTAFRFRLRRAAAALAAVAFLTASPAAAAKKKKRKEPEPTAVPR
                     PPDPARVTITTPDGVALAASWRPVDGNDRAPAVLLLHDFSRERRELEPLAHRLGEAGY
                     ATLAIDLRAHGESTRKNGQPIPISPRLLKDPNGFPRDVEAAAAWMKARAPKTAALGFS
                     VGGYLALLAAARGQVDAAVVISVNEPRVADLAGAATPVPRSLFLLACEADPGRADSAK
                     KLEAAAQAPKRVLLFPGAAHNLALLREHPEAEKAVLGWLAERLK"
     gene            13442..14134
                     /locus_tag="PLB01_08615"
     CDS             13442..14134
                     /locus_tag="PLB01_08615"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HQQ77405.1"
                     /translation="MKTVVFDIETVSFPWLELDPLQRERLTRRAEDHEEFLRMKEGGA
                     LSPYTGKVIVIAMMNPETGKGIVWHEAPGERASRASEDGFFEYVGCGEKDMLAEFWSA
                     LSKFDRFVTFNGRTFDGPFLSVRSAIHGLLPSKNLLGYRYSMETHVDLLEILTFQGAV
                     ARDQTPTLHSACVAFGIPSPKSEEMHGYAVGDAYHDGRLAEVAEYCRRDVEATAALFR
                     RLETTLLPLFKR"
     gene            14209..>14401
                     /locus_tag="PLB01_08620"
     CDS             14209..>14401
                     /locus_tag="PLB01_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HQQ77406.1"
                     /translation="MPAPSSPDPRAAWKAVVERDAAQDGRFVFAVATTGVYCRPSCAA
                     RRPNRENVRFFAGPGEAEAA"
BASE COUNT         2197 a         5116 c         4981 g         2107 t
ORIGIN      
        1 actccccgct tccgcgcatc gccttcgtcg gcctcggcac gatgggccgc ccgatggcgg
       61 cgaacctcgc gcgcgccggc ttccccctct ccctcgccac gcggactccg ggcaaggccg
      121 cgacgatcgc cgccgagctc tccggcggcg gccgcgacgt gcgcgcggcc gtgacgcccg
      181 cggacgccgc gcgcggggcc tccgtgatcg tctcgtgcgt cccggacgcg cccgaggtcg
      241 aggaggttca cctcggcccg ggcggcacgg ccgagacggc agcgcgggat accgtcgtga
      301 tcgactgctc gacgatcgac gccgcgaacg cccgcaccct cgccgccgct ctcgccgcga
      361 agggaatcgc attcctcgac gcgcccgtct ccggcggcca gaagggcgcg gtcgagggga
      421 cgctcacctt cttcgtcggc ggagacccgg ccgcactcga cggggcgcgg cccgtcttcg
      481 aggcgatggg caagcgcatc acgcacctcg ggccgtccgg cgccggacag ctcggcaagg
      541 cggcgaacca gatcctcgtc gcgaacaacc tcatggccgt ttccgaggcg ctcgcgttcg
      601 ccgcgaaggc gggcctcccg ctcgggccgc tgcacgaggc gctcgccggc ggcgcggcgc
      661 gctcgtgggc gctcgaggtc ctcggcgcga agatgatcga ccgcgacttc aagcccgcgt
      721 tcgcggtgaa gcaccagcag aaggacctcg cgatcgtcct ccggaacgcg cgcgccgcgg
      781 gagttccgct gccgggtgtc gccctcgtcc accagctcct cgcgtcgctc gaggccgagg
      841 gccgcggcga ggacggcacg caggcgctcc tcacggtcta cgagaagctc gccgccggac
      901 ggtaagattc gccccgaatg gagggcccca tgaaccggct tcggctgacc gctctcgtcg
      961 cagtgctcgt cgcgtcctcg gcggccctcg ccgagacgca gaagttcgaa cgcagctatc
     1021 ccttcgtcgg cgacaaggac gtcaaggtcg gcatcaaggt gggcgacgcc acgatcgagt
     1081 ccttccggat ccggcactgg ccggactccg acgatttcga gaaagcccgg aaggacctga
     1141 acgacacgca cacgatggtc gtggagttca cctattcgaa cagggaccgc gacaaggact
     1201 acaagtgcaa gtacgtggtc acgatcccgg ccggcaagga cggcaagccg ttcggcgaga
     1261 acgaccggac cgcgacgctc gacaagggca agatcgacga cacgaactcg atgttcgtga
     1321 agatgcgcac gaacgactac aaggttgcga agacgatcaa gatctccttc gagatctggc
     1381 ggaagtagcc cccggtctca ggcgcaggcg cgcgcgcgcg ggcgggcgtc ctgctcggcg
     1441 cgccaggcct cgcggcggcg ccggtagtcc tcgtcggcct ggcgggcgag gacgcgccgg
     1501 tagtcccgct tcaggcgcgc gacctgctcg gcgagcagca cgcggtcccc cgctccggcg
     1561 gcctggagct cgccctcgag gatctcgatc tgcgccctga ggacgcgcgc ttcgtgaagt
     1621 tccgccgcgg attccatgat tcccaagatc ccctttcctc gcggccccgg agggcctgct
     1681 gcctagagaa gacgcaattc gcgccgaaaa gtttcagccc caaaccggag ggcggctccc
     1741 cggcgtaaag aggacatgat gtcctcgcgt ttggctccga tcggattcgt tctgctcggg
     1801 gcggcaggag ttgccttggc caccgaagaa ccgaagtatc gcattgaaag ggattacgga
     1861 tcgttcgagg tccgcgtcta cgagcccacg gtcgtcgcgg agacggtcgt gacgggctcg
     1921 ttcggcgacg ggtcgaacga gggcttccgc cgtctcgccg gctacatctt cggcggcaac
     1981 gacggcggac ggaagatcgc gatgacggcc cccgtcggcg ccgagccggc cccccgctcg
     2041 aaggggacgt cgatcgcgat gaccgcgccc gtcgggtcgg agaggacggc cgagggctgg
     2101 gtcgtctcgt tcacgatgcc gtcgtccttc acgctgaaga cgctgcccgc ccccgacgac
     2161 gcgcgcgtga ggctgcgcga ggtgcccggg cggcgcgtgg ccgcggtgcg cttctcgggc
     2221 acgtggggcg cggagaagtt cgaggcgctc gcggcgaagc tggaatccga cgcgaagggg
     2281 accgggctct cccccgccga cgcgcccccg gtcttcgccc ggtacgaccc gccgtggacg
     2341 ccgtggttca tgaggcggaa cgaggtcctg atcccgctcg aagggcgctg accccggacg
     2401 accttctact cgagcgctcc gccgcacgag cttccggcgc ccgccgtgca gccgaagcag
     2461 tgacgccgcg tgacgaccgt ccgcgccgcg agagccgcga cgtcgaggtc ccggacgtgg
     2521 aaggcgccgt tcgagccgcg cgcgggcagg tcgagcatct ggttgaagtc gcaatcgtag
     2581 atccggccgt cccatccgat cgagagcgtg ttccggcaca tgaggccgtc gatcgtcgcc
     2641 gggttgaagg acgccacgag gagctcgagg tagctcgcga ggttcccgct cgactcgagc
     2701 cactcgaggt accgcgaaat cggcatgttg ttcaggaaga tgagccggtc gaacgcgacc
     2761 ccgtgctcgc ggaggaggcc ttccttccat tcgcgctcgg ccgcgcgctg gttcgccggg
     2821 aggaaggcgc cgacggggtt cgacacgagc gtcagcgtgc ggcgcgggtc ccccgagccg
     2881 tagcccgcct cgttcaggag atggagcgcc tggagcgact tctcgtacgt gccgtcgccg
     2941 cgctgggcgt cggtcccgcg cttcctccag tgcgggagcg agcagacgat ctcgacgccg
     3001 cgctcgccga gccacgcggg caggtcgcgg aagccggaca cgaggaggat cgtgaggttg
     3061 cagcggtcga tgacgtgctt gccgcgcgcg gccgcctcgt cgacgacgta ccggaaattc
     3121 gggttcagct ccggcgcgcc gcccgtcagg tccacggtgt gcgcacccgt ccggtcgagc
     3181 gccgcgagac aggcctccgc cgtcgcccgg tccatcattt cgtccgtccg gtccggcccg
     3241 gcgtcgacgt ggcagtggcg gcacgtcatg ttgcagagct tgccgaggtt gatctggaag
     3301 atctcgaccg ggccgggccg cagcgcgtcc cgcccgctcg cggcgagcgc gcgcgcgaag
     3361 tcgcctccgt gggccgacgc ggacaggtcc acggactcga ccgcggcgac ctgcttttcc
     3421 ggggcggcca gaggagcgcc ccgggcgtgc agggagagcg tcacatcgag gctttcttgg
     3481 cgtgctcgag catctgcatc ccgtgtacga gggaggcgcc tccgcggatc gccgccgtga
     3541 cgtggagggc ctcggtcatc tgctcgaggt ccgccccgtt cttcaggctc tcctgcgtgt
     3601 acgcgtcgat gcagtacggg cactggaccg cgtgggccac ggcgagcgcg atgagcgcct
     3661 tctcccgcgc cgagagcgcc ccgtcggcga agacggcgcc gtaccaggcg aagaacttgt
     3721 ccgcgagctc cttgttgccc tcggcgatcg aggcgaagtg cgcgagatgc tcggacttgt
     3781 aatagccgtc catgtgtccc ctctcgcgct cggcgcacgt ctacgcgcgg agcgtcttga
     3841 tcttgccgag gtgcccgtcg tcgtggttcg cgagacggac ggccagctgc tcgaccgtga
     3901 tctcgcccgc ctccgggtgc acgcccgtct tcagccagcc ctcgtgcggg agcgcccgga
     3961 gcagcgacac ggtgtcctcc ctcaggcgct tgaactcctt ggcgacctcg gccgggtcgg
     4021 cctcggcgta cttgagggtc tcggccgcgc ggtcgttgtc cagcatccag aacgacggct
     4081 tgtcgccgaa cgccatctgc gagaagcggc cctggtagac gcgctcgatg tcgcggaggt
     4141 gcgcggcgtt ctcgagcatg gaccacttcc cgccggcggc cttgcggcgg agcacgtcgt
     4201 ccggcgcgcc cttgagcgcg gcggcgagcg cggccggcga cgcggcgagg cgttcgagga
     4261 tcgcgtggcg gcgcttgatg gcctcgatct ggcggagatg cgcctcgtcg ttgccgaccg
     4321 cgtggcgctg gatgaggtct tccatcgtga gccggcccgc ggtcgcgtgg attccggccc
     4381 gcgcccactg ggccttgccc gccttgcgga gcagctggag cgtctggcgg cggagcgcca
     4441 cccactcgcg aacgacctcg ggcgccttct gggagcggta gcccctgagg atcgcgatcg
     4501 cctcgccgtc gaggttcggc agcgacggct cgttctcggt gaggatgcgc gtgtaacgct
     4561 cgatgtaggc ctcgcgctcc atgtcgcgca tgtggcagag gatctcgagg atggaccact
     4621 tgccgggcgc gggcgtccag aggaggacct tcttcgacaa ccccttgagg gcggccttga
     4681 gcgtctgcgg cgtcgtggcg agcgtctcga aatagcggcg gcgttcgaag tcttccatgg
     4741 ggtctcctca gccggcgcgg acgccgttca ggggtttgtc gagctccggg aacaggagct
     4801 cctgcggctt gagccgcggg agcagggtgt cgaggacgtt ctgcgaccag tcggcgcgcc
     4861 gggccgggtc gagtacgacg tgcgggacgc cccacacggc aagcagctgc tggatcgaac
     4921 ggtcgatgtc gtgctggaag gcgaggtcgg tgtcgcgcac accgtcgaag cgcgggtcgc
     4981 ccacgatcgg caccttgaag agggcgtcgt acgagcgcgt ccagctgcgc acgaggcctt
     5041 cgaggaccgc ttcttcgggg gcgccggtga acgcgcgctt catgtagcag aagttgtcga
     5101 gcaccgcacg gtcgcagaac acgacgtcgg ccttcgcctc cgcctgcagc tcccacgcga
     5161 tctgggtgtg gaggatccag gactgcgcct tgagcgtcgt gtcgcggttg atcgggagcg
     5221 ggcactcgcg cgcgacctcc cggacgagct cgaccgtgag gtcacgcttc ttgagcgccg
     5281 ccgcgaggtc gtaacagagc gtggtctttc cgaccccgtg ggtgccgatg aacgcgacct
     5341 tcatgcggga gcgagactac cgcacccggt cgggacgtga tccgcctcac tccggcgcgt
     5401 gcaggccatc gtctggcgcg cccggtccag gcctctccct cgatttccgc tgcgattagg
     5461 actagtttgc cgtggaaaag gaggccccca tgaagacccg tcacccgttc ctcgcgtcgg
     5521 tgctcgccgc ggtgcttctg ctcgccgtca gtcccatgcc cgcaccgatc tacatgaaca
     5581 tcccgaacgg ggcgacgctg gcgctccgga cgaaggtcga gttcaaacag gcggtcctct
     5641 gcgacggcag cgttcgtctc gagcccggct cgtacgacgt cagcttcaag tcgctcggcg
     5701 acggctccgt ccgcacgacg atcagcggca acgggaagac cggacacgcg accgggaagg
     5761 tcatggggca cgaagtcgtc gtccagggcg gcctgcagcc cggggcggcc gcgtccgttc
     5821 agaagatcca gcccggcgcc tccaacgcgg ctgccggtca ggggagcgcg caggcgaaca
     5881 ccccgccttc cttcgcgagc atggggttca cgccgcagag ccaggtctcg ctccggcaag
     5941 agggaaacaa attggacgtc atcgtgaacg gccaggggtc caaccagatc ctgatcggcc
     6001 tcctgctgcc cgcggtccag aaggtccgtg aagcggcgaa caagccccac tgacctcgag
     6061 gagagcacat gaagatccga cgtacgattc tcgcgtccct cctctcggcc gtcctccttc
     6121 tggccacgag ccccctgccc gcgcagacct acacgctgac gacgcaggct ccgcaagtga
     6181 cgaccatgac gccgttccag atcaacgtct acctcgcgca gccgacgctg tgcggcacga
     6241 cgaaggtcct ccttccaaag ggcgcatacg ccgtcaagat cgagtccctc ggcgccggca
     6301 gcgtgcgcgc gacgttcacg ggcaacggga agacctgtca gaaccccggg accctcacgg
     6361 ccagctcggg caacccgctg ggcgggacga cgagctcgta cgggaccacg accgccggcc
     6421 agtccttcgc ggccatgggc ttcacgccgc agagccaggt cgtcactcag acggtcggca
     6481 cgaagctcaa cgtgatcgtg aagggactgg gcacgaacca gatcctcatc ggcctcacgc
     6541 tgccgagcta ggggaagtga aatgagaagc gtcctcttcg ccctcgtcct ctccgcctca
     6601 accgccgcct ccgcgatgcc gaccgccgtc gagtcgggtc ccgtcgtcgc ggcgggccag
     6661 acgttccaga agggcgtcgt cctcgagacc gccgtgctct gcgacgggag cgtccgcctc
     6721 gagaaaggga cctacgacgt gaagttcgag tcgctcggca agaacaagat ccgggcgacg
     6781 ttcttccagg gcggcgcaag gaagggcgag gcgaacggca tcatcatcat cggcggcagc
     6841 aaccaggcct tgggcggcgt caggggcccg ggtcctggcc cggcccccgg cggcacgaac
     6901 gccttctcgt tcaacgacct cggccttcac ccgagctcgg cgcacgcgct ccagaagcag
     6961 ggcgacaagc tgaacctcgt cgtcaacggg cagggcacga atcagatcct gatcggcctc
     7021 cttcttcccg cggtccagaa gggcggcctc cagcccccgg gcatcaagcc gggcgcaccc
     7081 tccggaccgt aagcctcagc gcggcgcggg gaggccgacc tcggcgaaga gcctctccgt
     7141 gtcctcgccg aggcgcggcg gcgccgccgc cggcggatcg gagaatccct cgaggaagaa
     7201 gggcggacgg acggtgggga cgctccggtc gcctcttcgg gtctccagga cggatccgag
     7261 cgaacgcgcc tgcggcgcgt gaagagcctc gaggacgccg agcacgggtc cggcgggaat
     7321 tccacaagcc ttgcatcgcg cgagccacgc ctcgcgcggt tccctcgaaa aatgcgctgc
     7381 gaggatgggg acgagctcgt cccgattgac gacccgagca gcgttcgtct cgaaacgagc
     7441 gtccctcgag agctcgggcc gccccagaac cctttcgcag agaaatccga actgccggtc
     7501 cgtacccacg gcgagaagaa acggcgcatc cgccgcctcg aacatccggt agggctcgat
     7561 cgccgcgtgc gcgttgccgt gccgcgccgc ctctcgcccc gtcacgagcg ccgagccggc
     7621 tacgttgacg agagcggcga ggcccgtcga gacgagattc gtcttcacgt gagcgccggt
     7681 cccgtcgcgt tcgcgggcgt ggagcgccgc gaggatctcc gcgagcgcgg cccaccccgc
     7741 gaggacgtcc acgagcgcga cgcccatctt gtgcggcgcg ccgccggccg gccccgtgat
     7801 cgacatgagg cccgtcgcgc cctgcgcgag aaggtcgaaa cccggcgcgc cggcctcggg
     7861 gcccgcgtcg cccgcacccg agatcgagca ggcgacgagg tgcgggctcc gggcgcggag
     7921 cgcgtcgacg ccgatcccgc gtttctcgag cgcacccgga aggaagttct cgacgagaac
     7981 gtcggccttc gccgcccagc gccggacgaa ctcctgtccc tccggcgtgt cgagatccac
     8041 gacggccgat tccttgcgcc ggttcacgga gaggtagtac gccgcctcgc cgtcgagcgc
     8101 gggcggtttc cagccgcgcg agacgtcgcc gccgcgcgga tcctcgacct tgacgacgcg
     8161 cgccccgagg tccgcgagga gcatcgtcgc gagcggcccc gcgaggacgc gggagaggtc
     8221 gaggacgacg acgccgtcga ggggcggcct gcgcatcaga acagcggacg cttcacgacc
     8281 aggacggtcg tgtcgtcgga gggtgtgccg tcgcccacgt gccgccgcca gtccgcgaag
     8341 agcgcctcga cgatcgcctc cgcggggcgt cgccagaggc tcaccgcggt cagaacgtcg
     8401 cgcgggcggt cgtagccgta cggcgtgtcg acgaggtccg gcccttcgta gagcccgtcc
     8461 gacgcgagga tgaggacgcc gccccgggga atctcgacgg cgatgtcctc gtacgtgcgt
     8521 ttcggtccct gcccgagagg cagccccgag cccgtgatct cgcggcagac gccctcttcg
     8581 agcaggagcg ggtatgggtg gcccgcgttc gcgagaagcc cctttcccgt cttcgggtcg
     8641 agggcgagga gcgcgaccga cgtgaacagg cgaccgctgc gccccatgcg cagcacgcgg
     8701 tcgatctcga cgaggatctc ggacggcctg cgccccgcct tcacgaggct cgcgagagag
     8761 gccatgacca tcgcctgctg gatgccgcac gagtacccgt ggcccgacac gtcgcccgcc
     8821 gcgatccaga gcgagcccgc gtcgtcgacg acgaaatcga agagatcccc gcctgcctcc
     8881 gtcgcgagga cggtcttgac ggcgaggtcg aggccgtcga cggcgggcgg cttctcgggc
     8941 agcagcgaga gctgcatgcg cgagaggagg tccatctcgt acttcacgcg ccgctcggac
     9001 tcgagccgcc gcacgtactc gggagcgtcg aggcggccct cgtcctccga ttccggccgc
     9061 atccagccca cgacgcccgc caccgcgagc gcggcgaggg gaagcacggt cgcgagcgcc
     9121 gcgagcgcgt tgccgtcgag ggcgcgacat gcgaccagcg cgtccctcag gagcgccgac
     9181 gagaccgcgg cgacgaggag tgccgcgagg ccgtgcgcgt cgaacacgct gcggaaaacg
     9241 agggcgaaga ggaggcacag ggcgaggctc gctccccacg ggtgaagggg catcccggac
     9301 gacaggtaca gcgcgaagag gatcgctccc gccgtcccgg cccatgcgcg ggggaggacg
     9361 aaacgcagga gcgcgacgaa gagaacgaag atcgccgcgt cgaacgtgcc ctcgtagaag
     9421 ggccccttct gcgtctggaa gagggggtac gcgaacgacg gccccgtggg ccaggcgccc
     9481 cgcggggcgg cgagagcggc cgccgcgaac gcgaggagcg tgattcccac cgtcgcggcg
     9541 ccggcgccga ggcccgagag gatcgcgact ccgccgcgcg gcccgagccg ccgggccgcg
     9601 aaggagtcga ggctcgtcga gaaacccggc acggtgtccc ggaggagcga ctcggcgacg
     9661 gcccacagcg cgagcaggta gagcagcgag aagatccgga tcccgatcgc gaatgcgacg
     9721 aagccccctg ccgcaagttc ctctcccgag accccgccga gcacctcgta gagaagcgca
     9781 agcgccgcga ggaagatcgc gatccggaaa ccgaggcggc gccggtacag gaggaggccg
     9841 aacgcgaccc cgactgcgag gaacacgacg agggacggga tgaaccggta gaggcgccgc
     9901 gcgaggccct gggcgttgaa gtgctggggc gcctccgggt cgcgcagccg gcgcgcgacg
     9961 gaaagcttga ttcccccgga ttcgagggtc tggacgttct cggccggcgc gccgggcgcc
    10021 gcctccagcg gctggacgcg gaccatgacg ttcgtcgcgg gaaacgtcag ctcctcgccc
    10081 gccttcttcc ggccgccgcg cacgaggtcc tcgagcttcg cggcgaacgc gcggcgcgcg
    10141 acccgcgtct tctcgtcggg tccctcgaag atggaaccct cgggcgacca gtcgaccgcc
    10201 tggaccgagc cgtcgggcga cagggcgatc tcgacgggcc ccgcgccgcc ccccgggacc
    10261 gacacggcgc ccttcacggt ccacgcgaag gcccctcccg tcgtctcgag atacgtccgc
    10321 gcgtccgcgc ccagcctgcg gtaggcgcgc tcgaattccg acgcgaggtt cggccgcgcc
    10381 gagaacacga gcgtcgcccg ggtgagcgtc gctccggcgt cgcgcatcgc ggcgcgcccg
    10441 gactcgaccg cgttcaccgg gcgcggcggg accgcgcgcc actcgggcag gacggcgaac
    10501 gccgcggcga ggcccgcccc gaggaggacg atccccccga agagcgagac gcgcgggagg
    10561 cgcgcgctca agaccgggcg ccggccggcg cgagcgtctg ccccgccgtc atctcgagca
    10621 tccgctcgat ggacctcttc gccttcacgc ggacggcctc gggcacctcg atccgcggtg
    10681 cgaggtcggt gaggcaggcc gcgagcttct cgagcgtgtt gcgcttcatg tgcgggcagg
    10741 tgttgcacga gcagtccgcg accggcggcg ccgggatgag cgtcttgccg ggcgccgcga
    10801 gacgcatctg gtggaggatc ccttcctcgg tcgcgacgat gaacgcctgc ttcggggacg
    10861 tgacgacgcg cttcaagaga ccggacgtcg acgcgacgaa ggcggcgtgc gcgaggatgc
    10921 gcggctcgca ctcggggtgc gcgatgacct cggcccccgg gtggcgcgtc acgaggtcga
    10981 ggagcttctg ctccgagaac gtctcgtgga cgacgcacgt ccccggccag acgacgaact
    11041 tccggtccag cttcttctcg agccaagccg cgaggttccg gtccggcgcg aagacgacgg
    11101 ggtcgggaat ctgccggagg atgatctcgg cggacgacga cgtcacgatc acgtccgaca
    11161 gggccttcac gcccgccgag cagttgatgt agctcacgac cgtgtggccc gggtacttct
    11221 tgagccactc ggcgaagaga ggcgccgggc agccgtccgc gagcgagcag cccgcctcga
    11281 ggtccggcac gacgaccgtc ttttcgggcg tcaggatctt cgacgtctcg gccatgaagt
    11341 ggacgcccgc gaaggcgatg acggcctcgt tcgcctcctt gccgcggcgg gcgagctcga
    11401 gggagtcgcc cacgaagtcg gcgacgtcct ggatctcgga ctcctggtag taatgcgcca
    11461 ggacgatcgc gttccgctcc tccttcaggc gccggatgtc cagggcgacg tcctgagacg
    11521 ggatcgcact catgccgctc atcttaacgc cgggctgaac ctcgaaattc cctttgctac
    11581 actgcccgcg ccggcggccg gaaggcccgc ggggtttgaa tgagtgcacg cgctgaagcc
    11641 ttgtcccagc ctgccgcgtc cccgtggatc tcgaatccgc gctgggacct gatcgtcctc
    11701 tccttctccg ccgtactcgt ggccgtgccg ccgctcgtcc actccctcgg gaagacgacg
    11761 gccggcggcg tggacctcct catcacgctc ctcgtcggcg gcccccacat gtacgcgacc
    11821 ttcctgaaga cggtgttcga gccgaacttc cggaagctcc atccggcgct cacgttcgcg
    11881 cccgtcgtga tcattcccat gatcgtgatc tcgggcgccc tcttcgcgtt ccaggcgctc
    11941 ctcacgttct tcttcacgtg ggcctcgatg cacgtgtgcg accaggcttc gtacatcgcg
    12001 tacctctacc gcgagaaggg cggcgccgcg aagccctggc agcgcggcct tgacgtctcc
    12061 gtgatcctct tctccctcta cagctacgcg atgtaccggc acgtgaatgg gacgttcaag
    12121 atcggcgaca cgacgctctg gttcccggag ttcctcaagc acgcgtggtt cgcggacgcc
    12181 ttcgccctcg tgacggccgg actcatcgcc acctggatcg tctacacgct gaaccagctc
    12241 cggcgaaagg aagtcggagg cccgtacgtc ttcttcatgg cgctcacggt cggcgtcgcg
    12301 ctcgtcatcc cgcggatgcc cgagctctcg gtctccttcc agggcttcaa cgcgtggcac
    12361 tccttccagt acctcggcct cacgtacctg atcctccagc aggacaactc gatcgcgaag
    12421 gagagcctcg ccaagaggct cgcacagccc gggaagttct tccgctacta cggctggaac
    12481 atcctgctga cgaccggggc gacgctcatc atcgtcctcc tcacgatcgt cctgaagctg
    12541 cccgccgagc cgagctacta cgcggtcgtc ctgtcgttcc tcctcgtgca ctacgcgcac
    12601 gaccacgtgc tcttcgcctt cgggagcggc ttccgcccga agtgacggca ttccggttcc
    12661 gcctgcgccg cgcggccgcc gccctggcgg ccgtcgcgtt tctcacggcc tctccggccg
    12721 ccgccgcgaa gaaaaagaag cggaaggagc cggagccgac ggcggttccg cgcccgcccg
    12781 acccggcccg cgtgacgatc acgacgcccg acggcgtcgc cctcgcggcg tcgtggcggc
    12841 ccgtcgacgg gaacgaccgg gcgccggccg tcctcctcct ccacgatttc tcgcgcgagc
    12901 ggcgcgagct cgagccgctc gcgcacaggc tcggcgaggc cggctacgcg acgctcgcga
    12961 tcgacctgcg cgcccacggc gaatcgacga ggaagaacgg gcagccgatc ccgatctccc
    13021 cgcgcctcct caaggatccg aacggcttcc cgcgcgacgt cgaggccgcg gcggcgtgga
    13081 tgaaggcgcg cgcgccgaag acggcggcgc ttgggttctc ggtgggcggg tacctcgcgc
    13141 tcctcgcagc cgcgcgcggg caggtggacg ccgccgtcgt catctccgtg aacgagccgc
    13201 gcgtcgcgga cctcgccggg gcggcgaccc ccgtcccccg ctctctcttc ctcctcgcgt
    13261 gcgaggccga ccccggccgc gccgactcgg cgaagaagct cgaggcggcc gcgcaggccc
    13321 cgaagcgcgt gctgctcttc ccgggggccg cgcacaacct cgcgctcctg cgggagcacc
    13381 ccgaggccga gaaggccgtg ctcggctggc tcgccgagcg cctcaagtaa gcttccgccc
    13441 cgtgaagacg gtcgtgttcg acatcgagac ggtgtcgttt ccgtggctcg agctggaccc
    13501 cctccagcgc gagaggctca cgcgccgggc cgaagatcac gaagagtttc tgcgaatgaa
    13561 agagggcggg gcgctctctc cctataccgg caaggtcatc gtgattgcga tgatgaatcc
    13621 cgagacgggg aaagggatcg tctggcacga ggccccgggc gagcgcgcct cgagggcgtc
    13681 ggaagacgga ttcttcgaat atgtcggatg cggcgagaag gacatgctcg ccgagttctg
    13741 gtccgctctt tcgaagttcg accgcttcgt caccttcaac ggccgcacct tcgacgggcc
    13801 gttcctttcc gtgcggagcg cgatccatgg cctattacct tctaagaatc ttctcggcta
    13861 tcggtactcg atggagacgc acgtcgacct tctggagatc ctgacgttcc agggcgccgt
    13921 cgcgcgcgac cagaccccca cgctccactc cgcctgcgtc gcgttcggca tcccctcccc
    13981 gaagtccgag gagatgcacg gctacgccgt cggcgacgcg taccacgacg gtcggctcgc
    14041 cgaggtggcc gagtactgcc ggcgggacgt cgaggccacg gccgcgctct tccggcgcct
    14101 cgagacgacg ctcctgccgc tcttcaagcg ctgagcgagt tcggccgcaa gatccgggga
    14161 gaggaaggcc cttccctgcc gcacattggc ggcatgatga tcaccgcgat gcccgcgccg
    14221 tcctcccccg acccccgcgc cgcctggaag gccgtcgtcg aacgcgacgc ggcgcaggac
    14281 ggccgcttcg tgttcgccgt cgcgacgacg ggcgtctact gccgcccctc ctgcgcggcc
    14341 cgccgcccga accgcgagaa cgtccgcttc ttcgccggac ctggcgaggc cgaggccgcc
    14401 g
//
LOCUS       DAOMFY010000082        14327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1294_length_14327_cov_6.713215, whole genome shotgun sequence.
ACCESSION   DAOMFY010000082 DAOMFY010000000
VERSION     DAOMFY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14327
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1294_length_14327_cov_6.713215"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLB01_08625"
     CDS             <1..445
                     /locus_tag="PLB01_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77407.1"
                     /translation="LEPVDDDACLLAPGAAWTFSLARRDVPPLAATVGFPEGRLLEIY
                     GHDSSRRVSMVRFAAPGGASRIVRGFEFRKAASGLWGWASVRSTDFEVEESRAAYLLR
                     GRGTGHGAGLCQAGAIVRAKRGETRDAILSLYYRGASVVRLDSRA"
     gene            442..2154
                     /gene="mobB"
                     /locus_tag="PLB01_08630"
     CDS             442..2154
                     /gene="mobB"
                     /locus_tag="PLB01_08630"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HQQ77408.1"
                     /translation="MIRLESGLDVLLAGGAPHPRGRLALLCNPASVTLDFVPAPQALL
                     AAGLDVRVLFGPEHGLTGAAQDMEAVAAGEPSRLPAISLYGETFGDLSPKDEHLEDLD
                     AVVCDLPDVGSRYYTFVWTITLVMKECAKRKIPVWVLDRPNPLGGEVVEGNLPEEKLL
                     SFVGLHPVPVRHGMTPGEIARWTNATQGFGCDLTVVPMRRDGRAPTRREIAETPAWVL
                     PSPNMPTPETALVYPGGCLVEGTNLSEGRGTTRPFELLGAPWLDADDAAERANALGMP
                     GVAFRPHVFRPTFQKHAGRTCGGFQVHVTDAAAFRPYETYLRLLKALFDMDPDRFRWR
                     TEKYEYREDVPAIDLLTGTATYRRLVSEGASLDPWIASFRGDEARFVAQRRPHLLYPA
                     RRTSPAVLLVTGAHESGKTTVAVQIIESLVKQGLKVGSLKHTDHEYETDVEGKDSQRH
                     KAAGAEPAVLVAGRSSAVHRSAPGRPALSDFLEGEYGLRDRDVVIVEGFRGEVSYPKI
                     EVCRAATGRAPLCESDPNVVAVVTDFATRHNSSIPRFTFEETPGLLLLLRKSPLFSSL
                     PQNP"
     gene            2159..3112
                     /locus_tag="PLB01_08635"
     CDS             2159..3112
                     /locus_tag="PLB01_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HQQ77409.1"
                     /translation="MKAILVPKPGGPDALVYGDAPDPVPGPGEVLVRVRATAVNRADL
                     LQAEGKYPPPPGASEILGLEAAGEVEGTGEKVFFLLSGGGYAEKVAVPRGMLMPVPAR
                     LSLAEAAAIPEAWFTAYLNLFHEGGLKPGERLLVHAAASGVGTAAIQLAKRAGCSVVA
                     TARSEKKCEALSALGADIVVDTSRQEFLSRIEAKFGKDSVDLVLDSVGGPLFSPNIRS
                     LRRGGRIVLIASMAGPGAEIDLRAVLSKRLKIIGSTLRSRPIEEKVALTGAFVRDVLP
                     AFGDGSLAPVIDCAYPLRNAAEAHRRMAANENVGKIVLLVD"
     gene            3112..3768
                     /locus_tag="PLB01_08640"
     CDS             3112..3768
                     /locus_tag="PLB01_08640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQQ77410.1"
                     /translation="MAPIPAPPRAVVFDLDGTLLDSYAAIHECLALVLAAFGRPPVTP
                     AETRRMVGHGLEVLVAKAVGEANVAEGVRIFRERYERVGPETSVLLPGADAVTRRLVE
                     AGIPIAIASNKPARFSREILGTLGILSRFAFVGGPDDGFPPKPAPHMVFMALATMGAK
                     GRESVYVGDMPVDVATARAADVPVVAVPSGSSTETELRAVSPDLLIRDLNDLPGVLLS
                     "
     gene            3778..4908
                     /locus_tag="PLB01_08645"
     CDS             3778..4908
                     /locus_tag="PLB01_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HQQ77411.1"
                     /translation="MSNFDSTTGNAGQVRFFNPGPVWVRPQVLQALTGPMLSHRSQAF
                     MDLYGRILDKLPKVFRTTPGRAHTLASSATGVWEAALVSCVEGPVLSLGNGAFSEKWG
                     EMSQRLGLETDTLKAEWGKPISPDDVKAKLAAKKYAAVTVVHSETSTGVLNDLPAIAK
                     VVRENSDALVFADCVTSVAGTKVETDAWGLDVVLTGSQKAFALPPGLALFAMSERTLA
                     AAKKKKFRGLYLDLVDIDAFAQKKQTPTTPCLSLLYALDVQLDHILAEGIENRWARHE
                     AMRAAVADWAGKNGFSIFAQKGAESPTVTSMIPPAGVNAEELNKALKAAKWTTGSGYG
                     KLKATNVRIGHMGDVDVLSVQNYLKAHEEAVAALRAKAAVPA"
     gene            4905..6488
                     /gene="serA"
                     /locus_tag="PLB01_08650"
     CDS             4905..6488
                     /gene="serA"
                     /locus_tag="PLB01_08650"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQQ77412.1"
                     /translation="MNGRVLVAEPLSETGLAVLKTAGLAADVKTDLSREALLAVIGDY
                     DALVVRSATQVDKELLAAGKRLRVVGRAGVGLDNVDVKAATERGVLVVNAPSGNVVSA
                     AEHAIALLLALLRRIPEAQASLKAGEWKRTKFVGTELQGKTVGLVGLGQVGSRVAARL
                     RPWNLTLLAFDPFVTPERAAELGVKPVTLDELLAQSDVVSLHAPGGAETKHLLGPMQF
                     ERMKKGALLVNCARGALVDEAALVEALDSGKLGGAALDVFSVEPPKDFTLMKHPKVLC
                     TPHLGASTVEAQDRVAVETVEMLSEALKGSPFVAAVNLPFPAGGDPHAALPWMRLAEL
                     AGSFLGQAAEAAPTRISVTLSGVPEGARKAAVVAAVKGVLSGVEEVNLVNAAAIAKAR
                     GIALADSVREEASGYANLLTVAVDTPKGSRTVSATLFGDSHGRIVSLDGLSLEFKPQG
                     TMLVLSNQDVPGVIGRIGTCLGQKGVNIVDFALARGSGGRAAAVVRVDPPAGGIGPDL
                     VACLAGLEGVETARLVTIG"
     gene            6493..7611
                     /locus_tag="PLB01_08655"
     CDS             6493..7611
                     /locus_tag="PLB01_08655"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HQQ77413.1"
                     /translation="MTAPRVKSKTRVGVVFGGPSREHGVSFLSAKCLLENLPFEKYEA
                     IPVFVGKDGIWSGPKASKAELTRQLERPAKDLLVDGVKPGSRPARTAEIVDSFFSAWH
                     AAGADVLFPIVHGTFGEDGVVQGLFEALGIPYVGCGVAASAVGMDKIFMKAAFGAAGL
                     PQVKYVGFSRAEWASPASRAALTTRIDGLGFPVFVKPSCAGSSVGVSKVKDESQIAKA
                     VEAALLVDSRVLVEQGVDAREIECSVLEGEKGGPTEASLPGEIVPGHEFYDYEDKYID
                     SGSRSVIPADLTAGTKEEIRRLAARAFDVLGGEGFARVDFFLERGTQRILLSELNSLP
                     GFTPISMFPKMWEASGLPLGRLLDRLVTGAFHRPVRGR"
     gene            complement(7612..9531)
                     /locus_tag="PLB01_08660"
     CDS             complement(7612..9531)
                     /locus_tag="PLB01_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77414.1"
                     /translation="MSRTRWLAAVWIAAALVPLAVFHRTAVAPASRLVAALPSANPLD
                     DPATNATAIARAWARWERGDVRIADDRVFAPVPDALATGEWLPLASIVGYPFHALTGS
                     VPAGVNAAYFLAVFSFPVLLYAFYARLARPGLAPAVAAFAVAYGPGRMNTLGVLAGLS
                     TAFALLAAMAACDFLRKGRGRNLALFGAALVAQGLFSLYGIALGLVFAVPAVLIVAGK
                     EAFRPRRAAALAGTGLAAFLLLTIPYGPYFRIGESLGVVPGIRTFEAHSADLLSLLHG
                     GIFGGPVRDALERLVPGFPLGAAAFFPTLAFTFAIGLWAARGRSARSPIPWLGLAAAL
                     FLCSLGPTIHVAGRPIGPGPYRLLTGLPVLSSLRGIHRFDQWFDVALGAAAVLSLAAV
                     AGHAKAAAFRAAFTGLALLDLWPADIPATAFPAPSAYVDRLTALPRDASVALYPWNRS
                     TSTQAWIDQLSHGRRVVNGWFTYAPATHTWAERTLAALPVSGGVALLREMGASVIAVD
                     RRALPGPARADVDALAAGVGGIEAREEVAGFTLLRLARREPCFIRPGSPPLVFRGRTA
                     EAACRPDGLVFRAGPEQRDVLLRPSDGSPVRASLRAPVASPPPLRFTLSADAPAGTRV
                     EDAKSGRVLGQVAAR"
     gene            complement(9533..10228)
                     /locus_tag="PLB01_08665"
     CDS             complement(9533..10228)
                     /locus_tag="PLB01_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQQ77415.1"
                     /translation="MLFGLGLAACSSTPKAVSPAAAPAQGAIERGLASWYGKEFDGLP
                     TASGETFRPEKISAAHRTLPLGTVVDVTNEKNGRSVRVKVNDRGPFVAGRIVDLSKAA
                     AAEIGSVGDGVVPVTLRVVTLGDNSRIRAKSDEPPVPTPANTATKGEPGAPAVRPAST
                     GWAVQAGAFGSPENAVKLRERLAARYPSPWVEDFKGLRRVKFGPYATKAEAEAARDSL
                     GDLGLAGIVVEAR"
     gene            complement(10365..11819)
                     /gene="purB"
                     /locus_tag="PLB01_08670"
     CDS             complement(10365..11819)
                     /gene="purB"
                     /locus_tag="PLB01_08670"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQQ77416.1"
                     /translation="MNGSPADRWENPLTTRYASAEMSSLFSARSKFTAWRRLWLWLAE
                     AEKQLGLPIPEASLEALRAHLVPTDAELDAADRYERETKHDVMAHLHALGDVAPAAKG
                     VLHLGATSAFVGDNADLLLLREALRLLATRTVRVMGRLSRFARAYRDLPCLGFTHFQA
                     AQPTTVGKRACLWLQDLALDHRELVRRADELRFLGCKGATGTQASFVALFDGDADKAE
                     SLDKLVAEKAGFPDRLLISGQTYTRKQDALVLSALSGLASSASKFANDVRLLQHLKEV
                     EEPFGKKQVGSSAMPYKRNPMKCERMNALARWLLSVSDNGNWTHATQWFERTLDDSAN
                     RRLALAEAFLSADSILLLWDSVADGLVVHPSVVRRRLDVEIPFLATENILMAAAIKGG
                     DRQVLHEKIRLLAQAAGDRMKNEGGDNDLLFRIAADASFGLTPAEIQAAADPARFIGR
                     APEQVDEFLDVEIEPILATDPAAASGETHEEVRV"
     gene            complement(11816..11980)
                     /locus_tag="PLB01_08675"
     CDS             complement(11816..11980)
                     /locus_tag="PLB01_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77417.1"
                     /translation="MEEVAFVVCLNCDTPCYSFEYEERKGILSAFCAICGNDDVKEFR
                     LPTDDEVEEE"
     gene            12041..13321
                     /locus_tag="PLB01_08680"
     CDS             12041..13321
                     /locus_tag="PLB01_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HQQ77418.1"
                     /translation="MTLRIESEIGRLRAVLVHEPGREIDRMVPAMMQDLLFDDILFGA
                     RAREEHRRFRQVLKFVADEVFEARELLEEVLQDAGARNAILTELATLLAWPPAVLVAL
                     NDLPADRLAAGLVEGIEHPRPEITGSPDSLYLLPPLPNWFFQRDPAVVLGDRVIRSAM
                     ATRARWREPLLSGAIFAFHPRFGRRSDAFWFREFAAEEKSLSLARMRPTLEGGDVLVM
                     SAETLVIGYSERTEKITVERLAEALRKEKSPVRRILVVAIPPQRATMHLDTIFTRLSE
                     GECLCHAPMILAGGAEEVDVYEADLTGREVSWTTKDDLLSALRAHGIDLEPISCGGAD
                     PIDQQREQWTDGANLFALAPGLVLGYDRNIRTADELARRGYRILSDDDLLLGRETLER
                     KKPVKQAILMSAPELSRARGGPRCMTMPLVRDRV"
     gene            13335..14258
                     /gene="argF"
                     /locus_tag="PLB01_08685"
     CDS             13335..14258
                     /gene="argF"
                     /locus_tag="PLB01_08685"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQQ77419.1"
                     /translation="MRRKDLISIHDLTAAEVGALLVRAADIKARPREYRSALSGKILA
                     MIFEKSSTRTRVSFEAGMTQLGGAAQFLSSRDIQLGRGEPVSDTARVLSRYVDGIMAR
                     TFAHQTVLDLATYATIPVVNGLTDLLHPCQALTDYFTMSEKFGSLKGLKLTYVGDGNN
                     MAHSLMYGGPKCGVDVAVATPKGYEPHAGVTENARADAAAAGTKLLVTNDAEQAVEGA
                     HVVYTDVWASMGQEAEQAKRVRDFAGWTVSSALMARAHKDAVFMHCLPAHRGEEVAAD
                     VCDGPRSVIYDEAENRLHVQKAILVTLMGEP"
BASE COUNT         2115 a         5023 c         5139 g         2050 t
ORIGIN      
        1 cctcgagccg gtcgatgacg acgcgtgcct cttggcgcca ggcgccgcgt ggacgttctc
       61 gctcgcgcgc cgcgacgtgc cgcctctcgc cgcgaccgtg ggcttccccg aggggcgcct
      121 cctcgagatc tacggtcacg actccagccg gcgcgtctcg atggtgcggt tcgcggcgcc
      181 gggcggcgct tcgcgaatcg tgcgcggttt cgagttccgg aaggcggcgt ccggcctctg
      241 gggctgggcg tccgtgcgct cgacggactt cgaggtggag gaatcccgcg ccgcgtacct
      301 ccttcgcggc cgcggcacgg ggcacggcgc aggcctctgc caggctggcg cgatcgtgcg
      361 ggcgaagaga ggggagacgc gcgacgcgat cctctcgctc tattaccgcg gggcgtcggt
      421 cgtccggcta gactcgcgag cgtgatccgc ctcgagtccg gtctcgacgt cctcctggcc
      481 gggggcgccc cgcatccgcg cgggcgcctc gcccttctct gcaatccggc ttccgtcacc
      541 ctcgacttcg tccccgcgcc gcaggcgctc ctcgcggcgg ggctcgacgt gcgcgttctt
      601 ttcggtcccg agcacggcct gacgggtgcc gcgcaggaca tggaggccgt ggcggcggga
      661 gagccctctc gcctgcccgc gatctcgctc tatggcgaga cgttcggcga tctctccccg
      721 aaggacgagc acctcgagga cctcgacgcc gtcgtctgcg atctgcccga cgtcggaagc
      781 cgctactaca cgttcgtctg gacgatcacg ctcgtcatga aggagtgcgc gaaacggaag
      841 atccccgtct gggtgctcga ccggccgaac ccgctcggcg gcgaggtcgt cgaggggaac
      901 ctcccggagg agaagctcct gtcgttcgtc ggcctccatc cggtgcccgt gcggcacggg
      961 atgacgcccg gagagatcgc ccgctggacg aacgcgacgc agggattcgg ctgcgacctc
     1021 acggtcgtgc cgatgcgcag ggacggccgc gcgccgacgc gccgcgagat cgccgagacg
     1081 ccggcgtggg tgctcccgtc gccgaacatg ccgacgccgg aaacggcgct cgtctatccg
     1141 ggcggctgcc tcgtggaggg gacgaacctc tccgagggcc gcggcacgac gcgtccgttc
     1201 gagctgctcg gcgccccctg gctggacgcc gacgacgcgg cggagcgcgc gaacgcgctc
     1261 gggatgcccg gcgtcgcgtt ccggccgcac gtcttcaggc ccacgtttca gaagcacgcg
     1321 ggcaggacgt gcggcggctt tcaggtgcac gtcacggacg cggccgcgtt ccgtccctac
     1381 gagacgtacc tgcgcctcct caaggcgctg ttcgacatgg acccggaccg ctttcgctgg
     1441 cgcacggaga aatacgagta ccgcgaggac gtcccggcca tcgacctcct gacgggaacg
     1501 gcgacgtacc gccggctcgt cagcgaaggc gcgagcctgg atccctggat cgcgtccttc
     1561 cgcggcgacg aggcgcgctt cgtcgcgcag aggcggcccc acctgctcta tcccgctcga
     1621 aggacctccc cggcggtcct cctcgtcacg ggcgcgcatg agtcgggcaa gacgacggtg
     1681 gccgttcaga tcatcgaatc cctcgtgaag cagggcctga aggtcgggtc gctcaagcac
     1741 acggaccacg agtacgaaac agatgtcgaa ggcaaggact cccagcgcca caaggcggcc
     1801 ggagcagagc cagcggtcct ggttgctggg cgcagcagcg cggtccatcg ctcggcgccc
     1861 gggcgaccgg ctctttcgga tttcttagaa ggtgaatatg ggctgcgcga ccgtgacgtc
     1921 gtgatcgtcg agggatttcg cggcgaggtg tcgtacccga agatcgaagt ctgccgcgcc
     1981 gccacgggcc gcgctccgct ctgcgagagc gatccgaacg tcgtcgccgt cgtcacggac
     2041 ttcgcgacaa gacacaattc ttccatccca cgattcacat tcgaagaaac ccccggtctt
     2101 cttctccttc taagaaaatc tcctctcttc tcttctctcc cgcagaatcc gtaggctgat
     2161 gaaagccatc ctcgtcccca agcccggcgg accggacgcc ctcgtctacg gcgacgctcc
     2221 cgaccccgtc ccggggccgg gggaagtcct cgtccgcgtg cgcgcgacgg ccgtgaaccg
     2281 cgccgacctc ctgcaggccg agggaaagta cccgccgccg cccggtgcgt cggagatcct
     2341 cggcctcgag gcggcagggg aagtcgaggg cactggggag aaggtgttct tcctcctctc
     2401 cggcggcggc tacgcggaga aggtcgccgt cccccgcggc atgctgatgc ccgtgccggc
     2461 gcgcctgtcc ctcgccgaag ccgctgcgat tcccgaggcg tggttcacgg cgtacctgaa
     2521 cctcttccac gagggcgggc tcaagccggg cgagcggctg ctcgtccacg cggcggcctc
     2581 cggcgtcggg acggccgcga tccagctcgc gaagcgcgcg ggctgctccg tcgtcgcgac
     2641 ggcgcgctcg gagaagaaat gcgaggctct ctccgccctc ggcgcggaca tcgtcgtgga
     2701 cacgtcgcgc caggagttcc tgagcaggat cgaagcgaag ttcgggaagg attcggtcga
     2761 tctcgtcctc gactcggtcg gcgggccgct cttctcgccg aacatccgct cgctccgccg
     2821 cggcggacgc atcgtcctca tcgcgagcat ggccgggccg ggcgccgaga tcgacctgcg
     2881 cgccgttctg tccaagcgcc tgaagatcat cggatccacg ctacgctcgc gcccgatcga
     2941 ggagaaggtc gcgttgaccg gggcgttcgt ccgcgacgtg ctgccggcgt tcggcgacgg
     3001 gagcctcgca cccgtcatcg actgcgcgta cccgctgcgg aacgccgcgg aggcgcaccg
     3061 gcgaatggcg gcaaacgaga acgtgggcaa gatcgtcctt ctcgtggact gatggcgccc
     3121 atccccgcgc ccccccgcgc ggtcgtcttc gacctcgacg gcacgctcct cgattcctac
     3181 gccgcgatcc acgagtgcct cgcgctcgtc ctcgccgcct tcgggaggcc gcccgtcacg
     3241 cctgccgaga cgcgacggat ggtcggccac gggctcgagg tcctcgtcgc gaaggccgtc
     3301 ggcgaagcga acgtcgccga gggcgtgcgg atcttccgcg agcggtacga acgggtcggg
     3361 cccgaaacgt cggtcctcct ccccggcgcg gacgcggtga cgcgtcgcct cgtcgaggcg
     3421 ggcatcccga tcgcgatcgc gtcgaacaag cccgcgaggt tttcccgcga gatcctcggg
     3481 acgctcggca ttctgtcgcg tttcgcgttc gtcggcggcc cggacgacgg gtttcccccg
     3541 aagccggccc cccacatggt cttcatggcc ctcgcgacga tgggcgcgaa ggggagagag
     3601 agcgtctacg tcggggacat gcccgtcgac gtggcgacgg cccgggcggc ggacgttccg
     3661 gtcgtcgcgg ttccgagcgg aagctcgacg gagacggaat tgcgggccgt ttcacccgat
     3721 ctcctgataa gggatctcaa cgaccttccg ggcgtcctgc tttcgtagaa tcccgtcatg
     3781 agcaacttcg attcgaccac cggcaacgcc ggccaggtgc gcttcttcaa cccgggcccc
     3841 gtgtgggtcc ggccgcaggt gctgcaggcg ctcaccggcc ccatgctctc ccaccgctct
     3901 caggcgttca tggacctcta cgggcgcatc ctcgacaagc tcccgaaggt cttccggacc
     3961 acccccggcc gcgcgcacac gctcgcctcc tcggcgacgg gcgtgtggga agccgccctc
     4021 gtgtcctgcg tcgagggccc ggtcctctcg ctcggcaacg gagcgttctc cgagaagtgg
     4081 ggcgagatgt cccagcgcct cggtctcgag accgacacgc tcaaggccga gtggggtaag
     4141 cccatctctc ccgacgacgt gaaggcgaag ctcgcggcga agaagtacgc cgccgtgacg
     4201 gtcgtccaca gcgagacgtc cacgggcgtc ctgaacgatc ttccggcgat cgcgaaggtc
     4261 gtccgcgaga actccgacgc cctcgtcttc gccgactgcg tgacctcggt cgccggcacg
     4321 aaggtcgaga cggacgcgtg ggggctcgac gtcgtcctca cgggatccca gaaggcgttc
     4381 gcgctcccgc cgggcctcgc gctcttcgcg atgtccgagc ggacgctcgc ggccgcgaag
     4441 aagaagaagt tccgcggcct ctacctcgac ctcgtcgaca tcgacgcctt cgcccagaag
     4501 aagcagacgc cgacgacgcc gtgcctgtcc ctcctctacg cgctcgacgt ccagctcgac
     4561 cacatcctcg ccgaggggat cgagaaccgc tgggcccgcc acgaggccat gcgcgccgcc
     4621 gtggcggact gggcgggcaa gaacggcttc tcgatcttcg cgcagaaggg cgccgagtcg
     4681 ccgaccgtca cgtcgatgat cccgcccgca ggcgtcaacg ccgaggagct gaacaaggcg
     4741 ctcaaggcgg cgaagtggac gacgggctcg ggctacggaa agctcaaggc cacgaacgtc
     4801 cgcatcgggc acatgggcga cgtggacgtc ctttcggtgc agaactacct gaaggcccac
     4861 gaggaggccg tcgccgccct ccgtgccaag gctgccgtgc ccgcgtgaac gggcgcgttc
     4921 tcgtcgcgga acccctgtcg gaaacgggcc tcgccgtcct gaagaccgcg gggctcgcgg
     4981 ccgacgtcaa gaccgacctc tcgcgcgaag cgctcctcgc cgtgatcggc gactacgacg
     5041 ccctcgtcgt ccggtcggcc acgcaggtcg acaaggagct gctcgcggcg gggaagaggc
     5101 tgcgcgtcgt cgggcgcgcc ggcgtcggcc tcgacaacgt ggacgtgaag gccgcgaccg
     5161 agcgcggcgt cctcgtcgtg aacgctccgt ccgggaacgt cgtctccgcg gcggagcacg
     5221 cgatcgcgct cctgctcgcg ctcctgcgcc ggattcccga ggcacaggct tcgctgaagg
     5281 cgggagaatg gaagcgcacg aagttcgtcg gcaccgagct gcagggcaag accgtcggcc
     5341 tcgtcgggct ggggcaggtc ggctcgcgcg tggcggcgcg cctcaggccg tggaacctga
     5401 cgctcctcgc tttcgacccg ttcgtgacgc cggagcgcgc ggcggagctg ggcgtcaagc
     5461 cggtcacgct ggacgagctc ctcgcgcagt cggacgtcgt ctccctccac gcgccggggg
     5521 gcgccgagac caagcacctg ctcggtccga tgcagttcga gaggatgaag aagggcgccc
     5581 tcctcgtgaa ctgcgcgcgc ggcgcgctcg tggacgaggc ggcgctcgtc gaggccctgg
     5641 actccgggaa gctcggaggg gccgcgctgg acgtcttctc cgtggagccc ccgaaggact
     5701 tcacgctcat gaagcacccg aaggtgctct gcacgccgca cctcggcgcc tcgacggtgg
     5761 aggcgcagga ccgcgtcgcc gtggaaaccg tcgagatgct ctcggaggcc ctcaagggct
     5821 cgccgttcgt cgcggccgtc aacctgccgt tcccggcggg cggcgacccg cacgccgcgc
     5881 ttccctggat gcgtctcgcg gagctcgcgg gctcgttcct cggccaggcc gcggaggccg
     5941 ccccgacgcg gatctcggtc acgctctcgg gcgtcccgga gggcgcgcgg aaggccgcgg
     6001 tcgtcgccgc ggtgaagggg gttctctcgg gcgtcgagga agtcaacctc gtgaacgccg
     6061 cggcgatcgc gaaggcgcgc ggcatcgcgc tcgcggattc cgtgcgcgag gaagcctccg
     6121 gctacgcgaa cctgctgaca gtcgccgtgg acaccccgaa agggtctcgc acggtctcgg
     6181 cgacgctctt cggcgactcg cacgggcgca tcgtctctct cgacgggctc tccctcgagt
     6241 tcaagccgca ggggacgatg ctcgtcctct cgaaccagga cgttccgggc gtcatcgggc
     6301 gcatcggcac gtgcctcggc cagaagggcg tcaacatcgt cgacttcgcg ctcgcgcgcg
     6361 gaagcggcgg gcgcgccgcg gcggtcgtgc gggtggaccc gccggcgggg ggcatcgggc
     6421 ccgatctcgt ggcctgtctc gcggggctgg aaggcgtcga gaccgcccgg ctcgtgacga
     6481 ttgggtgacg ccgtgacggc gccccgggtg aagtcgaaga cgcgcgtcgg cgtcgtcttc
     6541 ggcgggccct cgcgcgagca cggcgtgtcg ttcctctcgg caaagtgcct gctcgagaac
     6601 ctgccgttcg agaaatacga ggcgatcccg gtgttcgtcg gcaaggacgg gatctggagc
     6661 gggcccaagg cctccaaggc cgagctgacg cgccagctgg agcggcctgc gaaggacctg
     6721 ctcgtcgacg gggtgaagcc cggcagccgg ccggcccgga ccgcggagat cgtggactcg
     6781 ttcttctcgg cgtggcacgc cgccggcgcc gacgtcctct tcccgatcgt gcacggcacg
     6841 ttcggcgagg acggcgtcgt ccagggcctc ttcgaggctc tcggaatccc ctacgtcggc
     6901 tgcggcgtcg ccgcgtcggc cgtcgggatg gacaagatct tcatgaaggc ggcgttcggc
     6961 gccgcggggc tcccgcaggt gaagtacgtg ggcttctcgc gtgccgagtg ggcctcgccc
     7021 gcgtcgcggg cggcgctcac gacgcggatc gacggcctgg gcttcccggt cttcgtgaag
     7081 ccgtcctgtg ccggctcgtc cgtcggggtc tcgaaggtga aggacgagtc gcagatcgcg
     7141 aaggcggtcg aagccgcgct tctcgtcgat tcgcgcgttc tcgtcgagca gggcgtcgac
     7201 gcgcgggaga tcgagtgctc ggtcctcgag ggcgaaaagg gcgggccgac ggaggcttcg
     7261 ctgcccggcg agatcgtgcc cggtcacgag ttctacgact acgaagacaa gtacatcgac
     7321 tcgggctcgc gcagcgtcat ccccgcggac ctgaccgcgg gcacgaagga ggagatccgg
     7381 cggctcgcgg cgcgggcctt cgacgtcctc gggggggagg gtttcgcccg cgtggacttc
     7441 ttcctcgagc gcgggacgca gcggattctc ctcagcgagc tcaactcgct gcccgggttc
     7501 acgccgatct cgatgttccc gaagatgtgg gaggcctcgg gcctgccgct cgggaggctc
     7561 ctcgaccgcc tcgtgaccgg cgcgttccac cgcccggtgc gcggccgctg atcagcgcgc
     7621 cgcgacctgt ccgagcaccc ggccggactt cgcgtcctcg acccgcgtgc ccgccggagc
     7681 gtcggccgag agggtgaatc ggagcggagg agggctcgcg acgggcgcgc ggagcgacgc
     7741 gcgcaccggg gagccgtccg acgggcgcag gagaacgtcg cgttgctcgg ggccggcccg
     7801 aaagacgagt ccgtccgggc ggcaggcggc ttcggcggtc cgcccccgga agacgagcgg
     7861 cggcgacccc ggccggatga aacacggttc gcggcgggcg agccggagga gcgtgaagcc
     7921 ggcgacctcc tcccgtgcct cgatcccgcc gactcctgcc gcgagggcgt cgacgtcggc
     7981 ccgcgcgggg cccgggagcg cgcgccggtc gaccgcgatg acggacgcac ccatctcgcg
     8041 aagcagcgcg acgccgcccg agaccgggag ggccgcgagc gtccgctccg cccacgtgtg
     8101 cgtcgcgggg gcgtaggtga accagccgtt gacgacgcga cgcccgtggc tcagctggtc
     8161 gatccatgcc tgcgtcgagg tggatcggtt ccaggggtag agggcgaccg acgcgtcgcg
     8221 cggaagcgcg gtcagccgat cgacgtaggc cgacggggcg ggaaacgcgg tggcgggaat
     8281 gtcggcgggc cagaggtcga ggagggccag ccccgtgaac gccgcacgga aagccgcggc
     8341 cttcgcgtgc ccggccacgg cggcgagcga cagcacggcc gcggccccga gcgcgacgtc
     8401 gaaccactgg tcgaaccggt ggattcccct cagggacgag aggacgggca gacccgtgag
     8461 caggcgatag ggcccgggac cgatcggccg gcccgcgacg tggatcgtcg gccccagcga
     8521 gcagagaaag agagcggcgg cgagcccgag ccacgggatc ggggagcgcg cgctccggcc
     8581 ccgcgcggcc cagaggccga tcgcgaacgt gaacgcgagc gtcgggaaga acgcggcggc
     8641 gccgagcggg aagcccggca cgagacgctc cagcgcgtcc cgcacgggcc cgccgaagat
     8701 cccgccgtga aggagcgaga ggaggtccgc ggagtgagcc tcgaacgtgc ggatgcccgg
     8761 cacgacgccg agagactcgc cgatgcggaa gtacggcccg tacggaatgg tcagaagcag
     8821 gaacgcggcg aggccggtcc ccgcgagagc agcggcgcgg cgcggccgga aggcctcctt
     8881 tccggcgacg atgaggacgg cggggacggc gaacacgaga cccagcgcga tcccgtagag
     8941 gctgaagagg ccctgggcga cgagcgccgc gccgaagagc gcgaggttcc ggccgcggcc
     9001 cttccggaga aaatcgcagg ccgccatcgc cgcgagcagg gcgaaggccg tggacaggcc
     9061 ggcgagaact cccagggtgt tcatgcggcc gggcccgtag gcgacggcga acgcggcgac
     9121 ggccggcgcg aggccgggcc tcgcgagccg cgcgtagaag gcatagagga gcacggggaa
     9181 gctgaagacg gcgaggaagt acgccgcgtt cacacccgcc ggcaccgaac cggtgagcgc
     9241 gtggaacggg tagccgacga tcgacgcgag ggggagccac tctccggtcg caagagcgtc
     9301 cggcaccggc gcgaagacac ggtcgtcggc gatgcggacg tctccgcgct cccagcgggc
     9361 ccacgcccgg gcgatcgcgg tggcgttcgt ggcggggtcg tcgagcgggt tcgccgaggg
     9421 cagggccgcg acgagccgcg atgcgggcgc gacggcggtc cggtgaaaga cggcgagcgg
     9481 gacgagcgcg gccgcaatcc agacggccgc gagccagcgt gtccgcgaca tctcagcgcg
     9541 cctcgacgac gatgccggcg agcccgaggt cgcccaggga gtcgcgcgcg gcctcggctt
     9601 cggccttcgt cgcgtaggga ccgaacttca cgcgccgcag gcccttgaag tcctcgaccc
     9661 acggcgacgg gtagcgcgcg gcgaggcgct cgcgcagctt caccgcgttc tccggcgagc
     9721 cgaacgctcc cgcctggacc gcccatccgg tgctcgcggg ccggacggcg ggcgcccccg
     9781 gctcgccctt cgtcgccgtg ttcgcgggcg tcgggacggg cggctcgtcg ctcttcgcgc
     9841 ggatcctcga attgtcgccg agcgtcacga cgcgcagcgt cacgggcacg acgccgtccc
     9901 cgaccgagcc gatctcggcg gcggcggcct tcgagaggtc cacgatgcgc ccggccacga
     9961 acgggccgcg gtcgttgacc ttcacgcgca cggacctccc gttcttctcg ttcgtgacgt
    10021 cgacgaccgt gccgagcggc agcgtgcggt gcgcggcgga gatcttctcg gggcggaacg
    10081 tctcgccgga cgccgtcggc agcccgtcga actccttccc gtaccacgac gcgaggccgc
    10141 gctcgatggc gccctgcgcc ggcgccgccg ccggtgagac cgccttcggt gtcgacgaac
    10201 aggcagcgag gccaagcccg aagagcacca gggagatttc tttgagtggg aagagggttg
    10261 ggcgccggcg gcgccacgcg gcgatggccg cgccgcgcgc agccgctcta ttcaccttca
    10321 aagaaaatct tctcttctct cttttctctt ctctcttctt cgcctcaaac ccgaacctct
    10381 tcgtgcgttt caccggaggc ggcggcgggg tccgtcgcga ggatgggctc gatctcgacg
    10441 tcgaggaact cgtccacctg ctccggcgcg cgcccgatga agcgggcagg atcggcggcc
    10501 gcctggatct cggccggggt cagcccgaac gacgcgtcgg ccgcgatccg gaacaggagg
    10561 tcgttgtcgc cgccctcgtt cttcatccgg tcgcccgcgg cctgcgcgag gagacggatc
    10621 ttctcgtgca ggacctgccg gtcgccgcct ttgatcgcgg ccgccatgag gatgttctcg
    10681 gtggcgagga acgggatctc gacgtcgagg cggcgccgga cgacggacgg gtggacgacg
    10741 aggccgtccg cgacggaatc ccacaggagc aggatcgagt ccgccgagag gaacgcctcg
    10801 gcgagcgcga ggcggcggtt cgcggagtcg tcgagcgtgc gctcgaacca ctgcgtcgcg
    10861 tgcgtccagt tcccgttgtc cgagaccgag aggagccagc gcgcgagcgc gttcatccgc
    10921 tcgcacttca tcggattgcg cttgtacggc atcgcggacg agccgacctg cttcttcccg
    10981 aacggctcct cgacctcctt gaggtgctgg agcagccgca cgtcgttcgc gaacttcgag
    11041 gcggaagagg cgaggcccga gagcgccgag aggacgagcg cgtcctgctt gcgcgtgtac
    11101 gtctgacccg agatcaggag gcggtcgggg aagcccgcct tctccgcgac gagcttgtcg
    11161 aggctctcgg ccttgtccgc atcgccgtcg aagagtgcga cgaaggaggc ctgggtgccg
    11221 gtggcgccct tgcagccgag gaagcgcagc tcgtcggcgc ggcggacgag ctcgcggtgg
    11281 tcgagcgcga gatcctgcag ccacaggcac gcgcgcttgc cgaccgtcgt gggctgcgcg
    11341 gcctggaagt gcgtgaagcc gaggcagggg aggtcgcggt aggcgcgcgc gaaccgcgac
    11401 agccgcccca tgacgcgcac ggtccgcgtc gcgaggagcc ggagcgcctc ccgcaggagc
    11461 agcaggtcgg cgttgtcgcc cacgaatgcc gacgtcgcgc cgagatggag gacgcccttg
    11521 gcggccgggg cgacgtcgcc cagcgcatgg aggtgcgcca tgacgtcgtg cttcgtctcg
    11581 cgttcgtagc ggtcggccgc gtcgagctcg gcgtccgtcg ggacgaggtg ggcacgcagg
    11641 gcctccagcg aagcctccgg gatcggcagg ccgagctgct tctcggcctc ggcgagccag
    11701 agccagagcc ggcgccacgc ggtgaacttc gaccgcgccg agaacaggga ggacatctcg
    11761 gcggacgcgt agcgcgtcgt gagggggttc tcccatcggt ccgcgggcga accgttcatt
    11821 cctcctccac ctcgtcgtcg gtcggaagcc ggaactcctt gacgtcgtcg ttgccgcaga
    11881 tggcgcagaa cgcgctcagg attcccttcc tctcctcgta ctcgaacgag tagcaggggg
    11941 tgtcgcagtt caggcagacg acgaaggcga cctcttccat gcgctctccc cgaaggccct
    12001 caggttagca tcaccgcggc ggccccgagc cgcgagttac atgacgttgc gcatcgaatc
    12061 cgaaatcggc cgcctgcgag ccgttctcgt ccacgagccc ggccgcgaga tcgaccggat
    12121 ggttccggcg atgatgcagg acctcctgtt cgacgacatc ctcttcgggg cccgcgcccg
    12181 cgaggagcac cggcgcttcc ggcaggtcct gaagttcgtc gcggacgagg ttttcgaggc
    12241 ccgggagctg ctcgaggagg tcctccagga cgcgggcgca cgcaacgcga tcctcacgga
    12301 gctcgcgacg ctgctcgcgt ggccgcccgc cgtgctcgtc gccctgaacg acctgcccgc
    12361 cgaccgtctc gcggccggcc tcgtggaggg catcgaacac ccgcggccgg aaatcacggg
    12421 ctcgccggac tccctctacc tcctgccgcc cctcccgaac tggttcttcc agcgggaccc
    12481 ggcggtcgtc ctcggggacc gcgtgatccg gagcgcgatg gcgacgcgcg cccgctggcg
    12541 cgagccgctt ctctcggggg ccattttcgc gttccacccg cgtttcggcc ggcgctccga
    12601 cgcgttctgg ttccgcgagt tcgcggcgga ggagaaaagc ctgtcgctcg cgcggatgcg
    12661 cccgacgctg gagggcggcg acgtcctcgt gatgtcggcc gagacgctcg tgatcggcta
    12721 ctcggagcgc acggagaaga ttacggtcga gcgcctcgcc gaggccctcc gcaaggagaa
    12781 gtcgcccgtc cggcggatcc tcgtggtcgc gatcccgccg cagcgggcga cgatgcacct
    12841 cgacacgata ttcacgaggc tctccgaagg agagtgcctc tgccacgcgc cgatgatcct
    12901 ggccggtggc gccgaggagg tcgacgtgta cgaggccgat ctcacggggc gcgaggtgtc
    12961 gtggacgacg aaggacgacc tcctgtctgc gctgcgtgcg cacgggatcg acctcgagcc
    13021 gatctcgtgc ggcggcgccg accccatcga ccagcagcgc gagcagtgga ccgacggcgc
    13081 gaacctcttc gcgctcgcgc cgggtctcgt gctcggctac gaccgcaaca tccggacggc
    13141 ggacgagctc gcgcggcgcg gttaccgcat cctttcggac gacgacctgc tgctcgggcg
    13201 cgagacgctc gaacgaaaaa aaccggtcaa acaggcgatc ctcatgtcgg ccccggagct
    13261 ctcccgcgcg cggggaggcc cccgctgcat gacgatgccg ctcgtgcgcg accgggtgtg
    13321 agaatggaaa cgccatgcga cgcaaagacc tgatctcgat ccacgacctc acggccgccg
    13381 aagtcggagc gctcctcgtg cgcgcggcgg acatcaaggc acgcccgcgc gagtaccgct
    13441 cggccctctc ggggaagatc ctcgcgatga tcttcgagaa atcctcgacg cggacccgcg
    13501 tctcgttcga agcgggaatg acgcagctcg gcggcgcggc ccagttcctc tcgtcgcgcg
    13561 acatccagct cgggcgcggc gagccggtct ccgacacggc gcgggtcctg tcgcgctacg
    13621 tcgacgggat catggcgcgc acgttcgcgc accagacggt cctcgacctc gcgacctacg
    13681 cgacgatccc ggtggtcaac gggctcacgg acctcctcca cccctgccag gcgctcacgg
    13741 actacttcac gatgtccgag aagttcggaa gcctcaaggg gctgaagctg acctacgtgg
    13801 gcgacggcaa caacatggcg cactccctca tgtacggcgg cccgaagtgc ggcgtggacg
    13861 tcgcggtcgc gacgccgaag ggctacgagc cccacgcagg ggtcacggag aacgcgaggg
    13921 ccgacgcggc ggccgcgggg acgaagttgc tcgtcacgaa cgacgccgaa caggccgtcg
    13981 agggcgcgca cgtcgtctac acggacgtct gggcgtcgat ggggcaggag gcggagcagg
    14041 cgaagagggt ccgggacttc gccggctgga ccgtctcgag cgcgctcatg gcccgcgcgc
    14101 acaaggacgc cgtcttcatg cattgcctgc ccgcgcaccg cggcgaggag gtggccgcgg
    14161 acgtctgcga cgggccgcgc tcggtcatct acgacgaagc ggagaaccgc ctccacgtgc
    14221 agaaggcgat cctcgtcacg ctgatgggag agccctgacc cgccgcgccc gcctcctcgc
    14281 cggggccgcg gcgatcgcgg tgctcgcgtc ctccgcgccg gggtgcc
//
LOCUS       DAOMFY010000083        13841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1356_length_13841_cov_8.002684, whole genome shotgun sequence.
ACCESSION   DAOMFY010000083 DAOMFY010000000
VERSION     DAOMFY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1356_length_13841_cov_8.002684"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLB01_08690"
     CDS             complement(<1..435)
                     /locus_tag="PLB01_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77420.1"
                     /translation="MKKARAAPDAGRRRGLAAALAFVALGIALRIATAYPVHKYAADA
                     DCLNCGYVGLRINDGHPAVWLTPRRAGALECYGHAVAFRAFGPTREALAITPLLSSSL
                     ALAVYAALVLPLLGPVAGPLAVLLFALPPPAYLFWTYMPNGYP"
     gene            complement(432..1385)
                     /locus_tag="PLB01_08695"
     CDS             complement(432..1385)
                     /locus_tag="PLB01_08695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ77421.1"
                     /translation="MSRVSVVVPVFHNAPSLPDLLARLAALAARHPSDDFEFVCVDDG
                     SKDDSLAVLVRLQAAEPRLRIVKLSRNFGSNAALLAGLAHANGDAIATISADLQDPPE
                     TLDAMLVRWREGKKVVLAVREGRDDPFATAFLANVFYRLFRRYAIRTMPEQGFDFFVV
                     DRSVRDLLLQIQESNAYLMGLVLWLGFDPACVPFRRQAREARYGSSMWGFGRKLKYFI
                     DSFVAFSYVPIRAASLLGLLVSVAGLLYALFIVVAHFFFDLKVQGWASLMVVLLIVSG
                     AQLLVMGVIGEYLWRNLDETRRRPRFIVEAVLEPEGRPRPS"
     gene            complement(1382..2119)
                     /locus_tag="PLB01_08700"
     CDS             complement(1382..2119)
                     /locus_tag="PLB01_08700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQQ77422.1"
                     /translation="MTQQAAPVLGRNAVIEPGARLGAGVAAGHNVTVHAGVVVGDGVF
                     LEDGAVLGRRPRTSGNTTRPVPADPGPLVLGPACVVGANAVLYAGSTLGARVMVGDLA
                     SVREGCRIADDVVLGRGVLVMYDTVVGARTRVIDGAILTGNMVIEEDVFIGPGVLTVN
                     DNEVYLRRFGLAPWAASGPRVKRFALIGTGANLVAGITVGVGAIVAPGAVVTRDVPDW
                     TIVAGVPARHMGPVADADRDAILRRFA"
     gene            complement(2131..3123)
                     /locus_tag="PLB01_08705"
     CDS             complement(2131..3123)
                     /locus_tag="PLB01_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ77423.1"
                     /translation="MIAAASGFSGARALVTGGAGFIGSNLVIALVERGADVTVADAMI
                     PGYGGNLFNLESVRTRIAFHEADIRDPEAMRKLVDGQDVVFHLAGQVDHVLSLTDPFP
                     DIDMNVKGTAVVMEALKHVNPRARVVFTGTRGQYGPAVSLPVSEDAPTNPRGIYEISN
                     LTAEKIIEVYDRVHGIPSSLLRLTNVYGPRAQMRHSRYGVVNWFVRLALDGETMHVFG
                     DGTIKRDFLYVDDCVEAILLAAAPDAPRAEVLNVGVDVPTTFRDLAVELQALTGARWE
                     YAPFSAERKAQEPGDFYSDVSKIRRLLGWSPRTPLTDGLERTLAFYRLHRAHYW"
     gene            3191..4192
                     /locus_tag="PLB01_08710"
     CDS             3191..4192
                     /locus_tag="PLB01_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ77424.1"
                     /translation="MKESFAQSLRCPRCRQSAWNLSIRQSDEREVREGELECRGCGRR
                     FGIERGIVDMLDPEDEALAREIAGWHEMAGPLEESLVPTMTALPWYPHEPWTHVAPDF
                     FQLFEHVNFTGLRVVDLGAGRTWSSRFLMAVGRAAELVAVDVLKKRFLGLETAEVFFG
                     QDGIFFERVCGDLHEIPVRDGWADAVFSCAAIHHSSDPGKVFREAWRVLKPGGRFVFI
                     SEPVKKKSILARRPDNEETRHGINEHIYTFDEYRDAWRAAGFRGGQLSPRTVRYRALY
                     PDADFQEGLPSLLARLMRSDHGRRLLARLLGGRFTGPLVYRYASLPLTCVLTRPSEG"
     gene            complement(4203..6053)
                     /locus_tag="PLB01_08715"
     CDS             complement(4203..6053)
                     /locus_tag="PLB01_08715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ77425.1"
                     /translation="MTPRRAAVVVLCWNRWDLTRRCLETLKAHTDLAEADVIAVDNGS
                     TDETPARLAELPWVRVVTNASNLGYVRGNNAGIAAAPPDADVVLLNNDVEIHQDGWLD
                     TLRETAHASADVGIVGCRLVLPDGRLLHAGTFILPDTFWGQQIGALETDVNQYAATRD
                     VEGIVFACAYVKREVLAAIGGLSEKFRSYFEDTDFCLRARDAGFRTVVCGAVTLVHHE
                     HGSTADQPKTFEAVFQESRKTFRSLWGKKLEARYRQELAWQSILNFPTGYAMSCREIL
                     RACEREGVRASYEYVYGPGTPFPVREDENARDYVLNVISARRSGRRPRVSVVYGQGDV
                     FHRNRGRHRIGYTMLEVDGFPEEWVKQANGMDEVWVPSRFNRDGFLASGLKKPVHVMP
                     LGVDVDHFHPAIRGVPNPAGDFVFLANFEWGERKRADMILKVFNQTFRRAESVALVCK
                     VINVNAATNVRAQIEALGLSTRGGRIAFLHNREFPYHQLGALYRSADCYVSAGRGEGW
                     DMPLMEAMACGLPSIATDWGAHTEFVHDGIAYPLKSRGLVPADARAPYYKGFRWADPD
                     PEHLAHLFRHVFEHRDEAAAKGRRAAAEMAAAWTWDHAARRIAARLDAIG"
     gene            complement(6050..6319)
                     /locus_tag="PLB01_08720"
     CDS             complement(6050..6319)
                     /locus_tag="PLB01_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77426.1"
                     /translation="MVSGADELERLRKEAAELRALSRELSAALARAVATLPLPAALAL
                     ARGGDAGADAPYPLDPWNETIEMTRSDVAPEMALLWRRLPAREGA"
     gene            complement(6313..6576)
                     /locus_tag="PLB01_08725"
     CDS             complement(6313..6576)
                     /locus_tag="PLB01_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77427.1"
                     /translation="MALHVPDDEWISARVEKARHEREMALAAASRLEDENRRLAYRVA
                     ELEADRVALKNRTEEAEAYVAAVKASAPWRVIQWLRGLVGRKW"
     gene            complement(6611..7696)
                     /locus_tag="PLB01_08730"
     CDS             complement(6611..7696)
                     /locus_tag="PLB01_08730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ77428.1"
                     /translation="MTEAGRLRVTWQSRWGLPIGYASTSEELARALLARDVDLAFRPT
                     PWPRTAHIADPVLEAISRRPPYEDAPQVSYEQADLFYTAHRSTRVGFTMLEVDGIPRA
                     WADACNAMDEVWTPSHWGAERFAESGVTKPIRVVPLGYDAGRFRPEPGARRASPRFTF
                     LSVFEWGERKAPEILLRAYAAAFSGADDVLLVVRANNHDGHVDVPRQIEDLGLPRNGP
                     PVAFLYNTHIRANQLAMLYQSADAFVLPSRGEGWGMPILEAMACGLPVIATPWSGQSE
                     FLSEDVGYPIALKGLVPADAKCPYYEGFRWAEPDLDDLVARFRHVFTHREEARAKGAR
                     AAQEAAAKWTWAHTAARVEERLRELGA"
     gene            complement(7693..8079)
                     /locus_tag="PLB01_08735"
     CDS             complement(7693..8079)
                     /locus_tag="PLB01_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77429.1"
                     /translation="MNVYWILSDDDAGNALEAVSRQVAEGDHGSPTLAGALGVLRFHA
                     EIFNDAVLETERPFFAPAINAFRRGLTKLFWWYGLPQWGQVSEYQHAVSSVIDILLAD
                     QRRLRTRVADLEKKLTELEARAGGKP"
     gene            8148..8981
                     /locus_tag="PLB01_08740"
     CDS             8148..8981
                     /locus_tag="PLB01_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ77430.1"
                     /translation="MTSLAPAPIVYDSARGGLLFVEEAKALWLYRHLVAEIVARDIKV
                     RYKRSVLGVAWTMLSPLLNMLAMTWVFSLVMRMDVKNFPVYFLTGSIFWAFFAGATSH
                     AASLTIDAAEITKRVYIPRSVFVVSAVGVALVNLCLSLVPLFLIVLFTGFPIHASWLF
                     VPVSILIGALFTTGVGLVVFTLASRFVDIKETYLVLVGAWFFVTPIVYTPSIVPERWR
                     FIVRYNPMTYLVEVFRAPLYDGWLPGPKTLAFAALAAVSSLAIGWLFYASRIEEYGRG
                     S"
     gene            8971..9723
                     /locus_tag="PLB01_08745"
     CDS             8971..9723
                     /locus_tag="PLB01_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ77431.1"
                     /translation="MAAEPAVELAGVSLSYRIPKEAVQTFKEYAIRKLQGRIEHGELV
                     ALRDATLSIAPGERVGVVGPNGAGKSTLFRVIARVRRPTRGRVVVRGRVAPLLELGLG
                     FHGELTGRENVVLHGAVMGHSRRDMERRMDAIAEFAELEAFLDAPLRTYSTGMAARLA
                     FAVATDVDPDILLVDEALAVGDERFQAKCHARMAGFHERGKTFLLVSHSLPEIVANCS
                     RAIWISGGRVVQDGPAAEVCAAYHAWAQGGTA"
     gene            9845..10798
                     /locus_tag="PLB01_08750"
     CDS             9845..10798
                     /locus_tag="PLB01_08750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ77432.1"
                     /translation="MAWCEECRAWFPVEDGFLDLLTGPLAYVEERDAFWSRHHAELTA
                     LGLSHAPSEARKGVPAVGDSAQALQQQKHFDWYAANETQRYSDYEVTPFWRAADRIAF
                     EPWKAELRASARPDQWLLDVGCAQGRSTFHMMDLDLNVVGFDVSRKCIREAARRYREG
                     TFRARASFLVADGAAFPFRDAAVDFVLVYGVLHHLADPAATCREIGRVLKSTGIYFGQ
                     ENNDSGFRALFDLAQKLWPIWHEEAGPEAIISADRLRSWLEPSGFRFSSRTSVFLPPH
                     VVNWTTEERGFRVLDVLDRAMAHVPWLRANGGLVIFEARRP"
     gene            10883..12508
                     /locus_tag="PLB01_08755"
     CDS             10883..12508
                     /locus_tag="PLB01_08755"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQQ77433.1"
                     /translation="MTRTGRLVALVAVALSGAVNAEAPDTTERVREWTAPPFWTPPVT
                     GASRTAEGRAALAPPPPTALPFIALTPCRVVDTRGNGAPLAGGFLPAATVRSYTVTGV
                     CGIPANAQALSLNATVVLPTGPGFLVLYPQGGTFPPVSTLNFLGNDVIVNAAVVPISG
                     AGGISMALGVSGSDVILDVNGYYAPTPAVTSLNALTGDVTLAAGSNVTLTPVGNTLTI
                     AATGGPGGSLPSGAANQTLRNTGSVWAASSALTNDGTNVALTGTLALPNPARITSGGS
                     TFVSTLGTQNTFVGINAGAFNTGQNNTGVGYQAMQNGGAGNLNTAFGQYAYLNGPSGN
                     INTAIGAQALQNCSAGDGNTAVGDTALYSLTSGSSNTALGWASGVSLTSGSSNLYLAN
                     FGAASESNTTRIGSAQTKAFIAGVRGVTTGSATGIPVLIDANGQLGTVSSSASVKHDI
                     RDMGELSARLMDLRPVAFRYNAQGEDAPVHFGLVAEEVNEVLPELVARNKAGEIETVM
                     YHELPAMLLNEVKKLRRAIDALRGEMEALKAAR"
     gene            complement(12630..13121)
                     /locus_tag="PLB01_08760"
     CDS             complement(12630..13121)
                     /locus_tag="PLB01_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77434.1"
                     /translation="MSRTRTCSPTLGISLLAAFAVLASSPPAVGADQPPSQKQAETPQ
                     AVRKESGTLVVTVEAPSSCEKTGDGHVVVPGIEVAAGRFEKKTGLDGRAKFTDLFPTT
                     YEVRAKPTDATGWVTAWVGPTPTAHVIPGQITFLTVRVCRRCTAGGNSLPGVPPASYP
                     VCR"
     gene            13324..13524
                     /locus_tag="PLB01_08765"
     CDS             13324..13524
                     /locus_tag="PLB01_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77435.1"
                     /translation="MTRSGRHDAPFATTPVPMILWGAASSPYFLSSVGSGAASMSFQV
                     RNAAGAVIDPETGFYFMAVQRF"
     gene            complement(13537..>13841)
                     /locus_tag="PLB01_08770"
     CDS             complement(13537..>13841)
                     /locus_tag="PLB01_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77436.1"
                     /translation="ALAAGGTRTFVLMGRCGVPSGATSVAANVTVTGSTAAGAVRVGP
                     AGTTPSLDTVAFRAGQTRANNAVLGLFGTPAGSVTVSTTIPSGTVHLVVDVTGYWQ"
BASE COUNT         2033 a         4842 c         4863 g         2103 t
ORIGIN      
        1 cgggtatccg ttcggcatgt acgtccagaa gaggtaggcc ggcggcggaa gggcgaagag
       61 gaggaccgcg agcgggcccg cgacggggcc gagaagcggg aggacgagcg ccgcgtagac
      121 cgcgagcgcg agcgagctcg acaggagcgg cgtgatcgca agcgcctcgc gcgtcggccc
      181 gaacgcgcgg aacgcgaccg cgtgcccgta gcactcgagg gcgccggctc gccgcggcgt
      241 gagccagacc gccgggtggc cgtcgttgat ccggagcccc acgtatccgc aattgaggca
      301 gtccgcgtcg gcggcgtact tgtgaacggg atacgcggtg gcaattctca gcgcgatccc
      361 gagcgcgacg aacgcgagcg cggcggcgag gccacggcgc cggccggcgt ccggggcggc
      421 gcgggccttc ttcacgaggg gcggggccgg ccttccggct cgagaaccgc ctccacgatg
      481 aagcgcggac ggcgcctcgt ctcgtcgagg ttccgccaca ggtattcgcc gatgacgccc
      541 atcacgagca gctgcgcgcc ggacacgatg aggagcacga ccatgagcga ggcccagccc
      601 tgcaccttga ggtcgaagaa gaagtgcgcg acgacgatga agagcgcgta caagaggccc
      661 gcgaccgaga cgaggaggcc gaggagcgac gccgcgcgga tcggcacgta ggagaacgcg
      721 acgaacgagt cgatgaagta cttcagcttg cgcccgaagc cccacatcga gctcccgtag
      781 cgggcctcgc gcgcctgccg cctgaagggc acgcacgcgg ggtcgaaccc gagccagagg
      841 acgaggccca tgaggtaggc gttgctctcc tggatctgca ggaggaggtc gcggacgctg
      901 cggtcgacga cgaagaagtc gaagccctgc tcgggcatcg tgcgaatcgc gtagcggcgg
      961 aagagccggt agaagacgtt cgcgaggaag gcggtcgcga acgggtcgtc ccggccctcg
     1021 cgcacggcga ggacgacctt cttcccctcg cgccagcgga ccagcatcgc gtcgagcgtc
     1081 tcgggggggt cctggaggtc ggcggagatc gtcgcgatcg cgtccccgtt cgcgtgggcg
     1141 agcccggcga ggagcgcggc gttcgacccg aagttgcgcg agagcttcac gatgcggagg
     1201 cgcggctcgg cggcctgcag gcgcacgagc acggcgaggg agtcgtcctt cgagccgtcg
     1261 tccacgcaca cgaactcgaa gtcgtcggac gggtggcgcg cggcgagcgc cgcgagccgc
     1321 gcgaggagat cgggcaggct cggcgcgttg tggaagaccg ggacgacgac cgagacgcgg
     1381 ctcatgcgaa acgccgaagg atggcgtcgc ggtcggcgtc cgcgacgggg cccatgtggc
     1441 gcgccggcac gcccgcgacg atggtccagt cgggcacgtc gcgggtcacg accgcgccgg
     1501 gcgcgacgat cgcgccgacg ccgacggtga tcccggccac gaggttcgcg cccgtgccga
     1561 tcagcgcgaa tcgcttcacg cgcgggcccg aggcggccca cggcgcgagg ccgaagcggc
     1621 gaagatagac ctcgttgtcg ttcacggtga ggacgccggg cccgatgaag acgtcctcct
     1681 cgatcaccat gttcccggtc aggatcgcgc cgtcgatcac acgcgtccgc gcgccgacga
     1741 cggtgtcgta catgacgagg acgccgcgcc cgagcacgac atcgtccgcg atgcggcagc
     1801 cttcgcgcac cgaggcgagg tcgcccacca tgacgcgagc gccgagcgtc gagccggcgt
     1861 agagaaccgc gttcgcaccg acgacgcagg ccgggccgag gacgagcggc cccgggtccg
     1921 cgggcacggg tcgcgtcgtg tttccgctcg tccgcgggcg gcggccgagg accgccccgt
     1981 cctcgagaaa gacgccgtcg ccgacgacga cgccggcatg gaccgtgacg ttgtggcctg
     2041 cggccacgcc ggcgccgagg cgggcgccgg gctcgatcac ggcgttccgg ccgagcacgg
     2101 gggcggcctg ctgcgtcatc tcccccaacc ctaccagtag tgggcccggt ggaggcggta
     2161 gaacgcgagc gtccgttcga ggccgtcggt gagcggcgtc cgcgggctcc agccgagaag
     2221 gcggcggatc ttcgagacgt ccgaatagaa gtcaccgggc tcctgcgcct tgcgctcggc
     2281 cgagaagggc gcgtactccc agcgcgcgcc ggtcagcgcc tggagctcga ccgcgagatc
     2341 ccggaacgtc gtcgggacgt cgacgcccac gttcaggacc tcggcgcgcg gcgcgtccgg
     2401 agcggcggcg aggaggatgg cctccacgca gtcgtccacg taaaggaagt cgcgcttgat
     2461 cgtgccgtcc ccgaagacgt gcatcgtctc gccgtcgagc gcgaggcgca cgaaccagtt
     2521 cacgacgccg tagcgcgagt gcctcatctg cgcgcgcggg ccgtagacgt tcgtgaggcg
     2581 cagcagcgac gaggggatcc cgtgcacgcg gtcgtagacc tcgatgatct tctcggccgt
     2641 gaggttcgag atctcgtaga tcccgcgcgg attcgtcggc gcgtcctcgg agacgggcag
     2701 cgaaaccgcg gggccgtatt gcccgcgcgt tcccgtgaag acgacccgcg cgcgcgggtt
     2761 gacgtgcttg agcgcctcca tgacgacggc cgtgcccttc acgttcatgt cgatgtcggg
     2821 gaacgggtcc gtgagggaga gcacgtgatc gacctggccc gcgaggtgga agacgacgtc
     2881 ctggccgtcg acgagtttcc tcatcgcctc cgggtcacga atgtcggcct cgtggaaggc
     2941 gatccgcgtg cggacgctct cgaggttgaa gagatttccg ccgtagcccg ggatcatcgc
     3001 gtcggccacc gtcacgtccg cgccgcgctc cacgagggcg atcacgaggt tcgagccgat
     3061 gaacccggcg ccgcccgtca cgagggcgcg cgcgccggaa aagccggagg cggcggcgat
     3121 catgggcgcg agcatagcgt cagggccggc ccgtgtatcg tttcccgcag ggaagccgga
     3181 tcacagaccg atgaaagagt cgttcgccca gagtctcagg tgtccccgtt gccggcagtc
     3241 cgcgtggaat ctctcgatcc ggcagagcga cgagcgggag gtccgggagg gcgagctcga
     3301 atgccgcggc tgcggccgcc gcttcggaat cgagcgcgga atcgtcgaca tgctggaccc
     3361 cgaggacgag gcgctcgcgc gggagatcgc cggctggcac gaaatggccg gtccgctcga
     3421 ggaatcgctc gtgccgacga tgaccgcgct cccctggtat ccccacgagc cgtggacgca
     3481 cgtcgccccg gacttcttcc agctcttcga gcacgtgaat ttcacggggc tccgcgtcgt
     3541 ggacctgggc gcgggccgga cgtggtcgtc ccggttcctg atggcggtcg gacgtgccgc
     3601 cgagctcgtc gcggtggacg tcctcaagaa gcggttcctc ggtctcgaga ccgcggaagt
     3661 cttcttcggc caggacggga tcttcttcga gagggtctgc ggcgacctcc acgagattcc
     3721 cgtgcgggac ggatgggcgg acgcggtgtt cagctgcgcg gcgatccacc attcttccga
     3781 ccccgggaag gtgttccggg aggcctggcg ggtcctgaag cccggcgggc gattcgtctt
     3841 catctcggag cccgtgaaga agaagtcgat cctggcccgc cggcccgaca acgaggaaac
     3901 gcgccacggg atcaacgagc acatctacac cttcgacgag taccgggacg cgtggcgcgc
     3961 ggccggcttc cgcggggggc aactctcgcc ccgaaccgtc cgctaccggg cgctctatcc
     4021 cgacgcggac ttccaggagg gtctgccgtc cttgctggcc cgccttatgc gttcggatca
     4081 cgggagacgg ctcctcgccc ggctcctcgg cgggcgcttc acgggtcctc tcgtgtaccg
     4141 ctacgcgagc ctcccgctga cgtgcgtcct gaccaggccg tccgaagggt aggaacgcgc
     4201 ccctacccga tcgcgtcgag ccgcgccgcg atgcggcgcg ccgcgtggtc ccacgtccac
     4261 gcggccgcca tttcggccgc cgcgcggcgg cccttcgcgg cggcttcgtc gcgatgctcg
     4321 aagacgtggc ggaagaggtg cgcgaggtgc tcgggatcgg ggtcggccca gcggaagccc
     4381 ttgtagtacg gggcgcgcgc gtcggccggt acgaggccgc ggctcttcag cgggtacgcg
     4441 atcccgtcgt ggacgaactc ggtgtgcgcg ccccagtccg tcgcgatcga cggcagtccg
     4501 cacgccatcg cctccatgag cggcatgtcc cagccctcgc cgcgccccgc ggacacgtag
     4561 cagtccgccg agcgatagag cgcgcccagc tggtggtacg ggaactcccg gttgtggagg
     4621 aacgcgatgc gcccgccgcg ggtcgagagg ccgagcgcct cgatctgcgc cctcacgttc
     4681 gtcgccgcgt tcacgttgat gactttgcag acgagggcga cgctctccgc gcgccggaac
     4741 gtctggttga agaccttgag gatcatgtcc gcgcgcttcc tctcgcccca ctcgaagttc
     4801 gcgaggaaga cgaagtcgcc cgcggggttc ggcacgccgc ggattgccgg gtggaagtgg
     4861 tcgacgtcga cgccgagcgg catcacgtgg accggcttct tcaggcccga ggcgaggaac
     4921 ccgtcgcggt tgaaccggga cggcacccac acctcgtcca tcccgttcgc ctgcttcacc
     4981 cactcctcgg ggaagccgtc cacctcgagc atcgtgtagc cgatccggtg gcggcccctg
     5041 ttgcggtgga acacgtcgcc ctggccgtag acgacggaca cgcgcggccg ccggccgctc
     5101 cggcgcgcgg agatcacgtt caggacgtag tcgcgggcgt tctcgtcctc gcgcaccgga
     5161 aagggcgtgc ccggaccgta gacgtactcg taggaggcgc ggactccttc ccgctcgcag
     5221 gcccggagga tttcgcggca gctcatcgcg tagccggtgg ggaagttcag gatcgactgc
     5281 caggcgagct cctgccggta ccgggcctcg agcttcttcc cccagaggct gcgaaacgtc
     5341 ttccggctct cctggaagac ggcctcgaac gtcttcggct gatccgcggt cgagccgtgt
     5401 tcgtggtgga cgagcgtgac ggcgccgcac accaccgtcc ggaaccccgc gtccctcgcg
     5461 cgcaggcaga agtccgtgtc ctcgaagtac gagcggaact tctcggagag cccgccgatt
     5521 gcggcgagca cctcgcgctt cacgtacgcg cacgcgaaga cgatcccctc gacgtcgcgc
     5581 gtcgcggcgt actggttcac gtccgtctcg agcgcgccga tctgctggcc ccagaacgtg
     5641 tcgggcagga tgaacgtccc cgcatggagg agccgcccgt ccgggaggac gagccggcag
     5701 ccgacgatcc cgacgtccgc cgacgcgtgg gccgtttccc ggagcgtgtc gagccagccg
     5761 tcctggtgga tctccacgtc gttgttcagg aggacgacgt cggcatcggg cggcgccgcg
     5821 gcgatgcccg cgttgttccc gcgcacgtac ccgaggttcg acgcgttcgt cacgacgcgc
     5881 acccacggca gctcggcgag gcgcgcgggc gtctcgtccg tcgagccgtt gtcgacggcg
     5941 atgacgtccg cctccgcgag gtcggtgtgc gccttcagcg tttcgaggca gcggcgcgtc
     6001 aggtcccagc ggttccagca gaggacgacg acggccgccc ggcggggcgt cacgcgccct
     6061 cccgggccgg gaggcgcctc cacaggagtg ccatttcggg cgcgacgtcg gagcgtgtca
     6121 tctcgatcgt ctcgttccag gggtcgagcg ggtacggcgc atcggctccc gcgtcgccgc
     6181 ccctcgcgag cgcgagcgcc gcgggcaggg gcagggtcgc caccgcgcgc gcgagggccg
     6241 ccgagagctc gcgcgagagg gcgcgcagct ccgcggcctc cttcctcagg cgctccagct
     6301 cgtcggcgcc gctcaccatt tccggcccac gaggccgcgc agccactgga tcacgcgcca
     6361 cggggccgac gccttgacgg ccgcgacgta cgcctcggcc tcctccgtgc ggttcttcag
     6421 cgccacgcgg tcggcctcga gctccgcgac gcggtatgcg aggcgccggt tctcgtcctc
     6481 gagccggctc gccgcggcga gcgccatctc gcgctcgtgc cgcgcctttt cgacgcgcgc
     6541 cgagatccac tcgtcgtcgg gaacgtggag ggccacgccg gaagtatagg aggcgggccg
     6601 gtccgcggcg tcaggcgccg agctcgcgca gccgctcctc gacgcgggcg gccgtgtgcg
     6661 cccatgtcca cttcgcggcc gcttcctgcg cggcccgggc gccctttgcg cgggcctcct
     6721 cgcggtgcgt gaagacgtgc cggaaccggg ccacgagatc gtcgaggtcg ggctcggccc
     6781 agcggaaccc ctcgtagtac gggcacttcg cgtccgcggg gacgaggccc ttcagcgcga
     6841 tggggtagcc gacgtcctcg ctcaggaact ccgattggcc gctccacggc gtcgcgatca
     6901 ccgggaggcc gcaggccatc gcctcgagga tcggcatccc ccagccttcg ccgcgcgacg
     6961 ggagcacgaa cgcgtctgcg ctctggtaga gcatcgcgag ctggttcgca cggatgtgcg
     7021 tgttgtagag gaacgccacc ggcgggccgt tccgcggcag gccgagatcc tcgatctgcc
     7081 ggggcacgtc gacgtgcccg tcgtggttgt tcgcgcggac gacgaggagg acgtcgtcgg
     7141 cccccgagaa cgccgccgca taggcgcgca gcaggatctc cggcgccttg cgctcgcccc
     7201 attcgaagac cgagaggaac gtgaaccgcg gactcgcccg gcgggccccg ggctccggcc
     7261 ggaaccgccc cgcgtcgtag ccgagcggca cgacgcggat cggcttcgtg acgccgctct
     7321 cggcgaaccg ctcggcgccc cagtgagagg gcgtccacac ctcgtccatc gcgttgcagg
     7381 cgtccgccca cgcacgcggg attccgtcca cctcgagcat cgtgaacccg acgcgagtgc
     7441 tgcggtgggc cgtgtagaag aggtcggcct gctcgtagct cacctggggc gcgtcctcgt
     7501 acggaggtcg ccgggagatc gcctcgagga cggggtccgc gatgtgggcc gtcctcggcc
     7561 agggcgtcgg ccggaacgcg agatcgacgt cgcgcgcgag gagcgcgcgc gcgagttcct
     7621 cggacgtgct cgcgtacccg atcggcagcc cccagcgcga ctgccatgtc acccggaggc
     7681 gcccggcctc cgtcacggct tcccgcccgc gcgcgcctcg agctccgtca gcttcttctc
     7741 cagatccgcg acgcgcgtcc gcaggcgccg ctggtcggcg aggaggatgt cgatcacgct
     7801 cgagacggcg tgctggtact ccgagacctg cccccactgc gggaggccgt accaccagaa
     7861 cagcttcgtg aggccgcggc ggaacgcgtt gatcgcgggt gcgaagaacg gccgctcggt
     7921 ctcgaggacg gcgtcgttga agatctccgc gtggaagcgg agaacgccga gcgcgccggc
     7981 gagcgtcggg ctcccgtggt cgccctcggc gacctggcgc gatacggcct ccagggcgtt
     8041 cccggcgtcg tcgtccgaga ggatccagta gacgttcacg atcggcctcg cgggcaggat
     8101 cgagccctcc gggcgggcgt gtcaagcgat ccggctagac tcgccccgtg acgtctctgg
     8161 ccccggctcc gatcgtctac gacagcgccc gcggaggcct cctcttcgtc gaggaggcga
     8221 aggccctctg gctctaccgc cacctcgtcg cggagatcgt cgcgcgcgac atcaaggtcc
     8281 gctacaagcg ctccgtcctc ggcgtggcgt ggaccatgct gagcccgctc ctgaacatgc
     8341 tcgcgatgac gtgggtgttc tccctcgtca tgcggatgga cgtgaagaac ttcccggtct
     8401 acttcctcac gggctcgatc ttctgggcct tcttcgcggg cgcgacgagt cacgcggcgt
     8461 cgctcacgat cgacgcggcc gagatcacga agcgcgtcta catcccgcgc tcggtcttcg
     8521 tcgtctcggc ggtcggggtc gccctcgtga acctctgcct ctcgctcgtt ccgctcttcc
     8581 tcatcgtcct cttcacgggg tttccgatcc acgcgtcgtg gctcttcgtg cccgtgtcga
     8641 tcctgatcgg cgcgctcttc acgacggggg tcggcctcgt ggtgttcacg ctggcctcgc
     8701 gcttcgtcga catcaaggaa acctacctcg tcctcgtggg cgcctggttc ttcgtgacgc
     8761 cgatcgtcta cacgccgtcg atcgtccccg aacgctggcg cttcatcgtc cggtacaacc
     8821 cgatgacgta cctcgtcgag gtcttccggg cgccgctcta cgacggctgg ctcccggggc
     8881 cgaagacgct cgcgttcgcg gcgctcgccg cggtctcgtc tctcgcgatc ggctggctct
     8941 tctacgcctc ccgcatcgag gagtacggcc gtggcagctg agcccgccgt cgagctcgcg
     9001 ggcgtctccc tgagctaccg catcccgaag gaggcggtgc agacgttcaa ggagtacgcg
     9061 atccgcaagc tccagggccg aatcgagcac ggcgagctcg tcgcgctccg cgacgcgacg
     9121 ctctcgatcg cgccgggcga gcgcgtcgga gtcgtcggcc cgaacggcgc gggcaagagc
     9181 acgctcttcc gcgtcatcgc ccgcgtccgc cggcccacgc gggggcgcgt cgtcgtccgc
     9241 gggcgcgtcg cgccgctcct cgagctcggc ctcggcttcc acggcgagct cacgggccgc
     9301 gagaacgtcg tcctccacgg cgccgtcatg ggccactcgc ggagggacat ggagcggcgc
     9361 atggacgcga tcgccgagtt cgcggagctc gaggccttcc tcgacgcgcc gctcaggacg
     9421 tactcgacgg gcatggcggc ccgccttgcg ttcgcggtcg cgaccgacgt cgacccggac
     9481 atcctcctcg tcgacgaggc cctcgcggtg ggcgacgagc gcttccaggc caagtgccac
     9541 gcccgcatgg ccgggttcca cgagcgcggg aagacgttcc tcctcgtgtc gcactcgctg
     9601 cccgaaatcg tcgcgaactg ctcgcgggcg atctggatct cgggagggcg cgtcgtccag
     9661 gacggccccg ccgcggaggt ctgcgccgcg taccacgcgt gggcgcaggg aggaacggcg
     9721 tgagccggct ggagatcctt tcctgagggt cgaggttttt ccccggctgc gttgccccgt
     9781 ctgccgctcg ggagggctcg cgtggcgcga gttcgtccgg gcgggcgacg agctgctgga
     9841 gggcgtggcc tggtgcgagg agtgccgcgc gtggttcccc gtcgaggacg gcttcctgga
     9901 cctcctcacg ggcccgctcg cgtacgttga ggaacgggac gccttctgga gccgccacca
     9961 tgcggagctc acggcgctcg gcctctcgca cgcgccgtcg gaagcgcgga agggcgttcc
    10021 ggcggtcggc gattccgccc aggcgctcca gcagcagaag cacttcgact ggtacgccgc
    10081 gaacgaaacg cagcgctact ccgactacga ggtcacgccg ttctggcggg cggccgaccg
    10141 gatcgcgttc gagccgtgga aagcggagct ccgcgcgtct gcgcggcccg atcagtggct
    10201 cctcgacgtc ggctgcgcgc aggggcgcag cacgttccac atgatggacc tcgacctgaa
    10261 cgtcgtcggg ttcgacgtct cgcgcaagtg catccgcgag gccgcgcggc gataccggga
    10321 agggacgttc agggcgcggg cgagcttcct cgtcgcggac ggagcggcgt ttccgttccg
    10381 ggacgcggcg gtcgatttcg tcctcgtcta cggcgtgctt caccacctcg cggatcccgc
    10441 cgcgacgtgc cgggagatcg gccgcgtcct gaagtccacg ggtatttact tcgggcagga
    10501 gaacaacgac agcggcttca gggccctctt cgacctcgcg cagaagctgt ggcccatctg
    10561 gcacgaggag gccgggcccg aggccatcat ctcggccgac cggctcagga gctggctcga
    10621 gccgtccggg ttccggttct cgtcgcgcac gagcgtcttt ctcccgcccc acgtcgtgaa
    10681 ctggacgacg gaagagcgcg ggttccgggt cctcgacgtc ctggatcgcg cgatggctca
    10741 cgtcccctgg ctccgcgcga acggcggtct cgtcatcttc gaagcgcggc ggccgtaggg
    10801 aatctcaaca ctcgccttgg gcttgcgttt tttccctcct tgtcattagg atcgcgtctc
    10861 aatccaaaat aggagtagcg aaatgacgcg aaccgggcgc cttgttgccc ttgtggccgt
    10921 ggctctttcg ggagccgtta atgcagaagc ccccgacacg acggagcgcg tcagggagtg
    10981 gacggcgccc ccgttctgga cgccgccggt gacaggcgct tcccggacgg ccgaagggcg
    11041 cgcggcgctc gcgccgccgc cgccgaccgc gctgccgttc atcgcgctca cgccgtgccg
    11101 cgttgtcgac acgcgcggca acggcgcgcc gctcgcgggc gggttcctgc cggcggcgac
    11161 ggtgaggagc tacacggtca cgggggtgtg cggcattccg gccaacgcgc aggcgctgtc
    11221 gctcaacgcc accgtcgtcc tgccgacggg cccggggttc ctcgtcctct acccgcaggg
    11281 tgggactttt ccgccggtgt cgacactgaa cttcctcggg aacgacgtga tcgtgaacgc
    11341 cgcggtggtc ccgatcagcg gcgccggcgg gatttcgatg gcgctcggcg tctcgggcag
    11401 cgacgtcatc ctcgacgtga acggctacta cgcgccgacg ccggccgtca cgagcctgaa
    11461 cgcgctgacg ggcgacgtca cgctcgcggc gggcagcaac gtcacgctca ctcccgtcgg
    11521 caacacgctg acgatcgccg ccacaggcgg tccgggcggc tccctgccct cgggcgccgc
    11581 gaatcagacg ctccgcaaca cgggctcggt ctgggccgcc tcgagcgctc tcacgaacga
    11641 cggcacgaac gtcgccctca cgggtaccct tgccctgccg aatcccgcca ggatcaccag
    11701 cgggggctcc actttcgtga gcacgctcgg gacgcagaac acgttcgtcg gaatcaacgc
    11761 aggggccttc aacaccggcc agaacaacac cggcgtcggc taccaggcca tgcagaacgg
    11821 cggtgcaggc aacctcaaca cggctttcgg acagtacgcc tacctcaacg gcccgagcgg
    11881 aaacatcaac acggcgatcg gcgcccaggc actgcaaaac tgttcggcgg gcgacgggaa
    11941 taccgctgtt ggtgacaccg ctctctattc gctgacgtcg ggctcctcga acacggcgct
    12001 cggctgggcg tctggcgttt cgttgaccag cggcagcagc aacctctatc tcgcgaactt
    12061 cggggcggcc tccgaatcca acacgacacg catcgggagc gcccagacga aggccttcat
    12121 cgccggcgtc cgcggcgtca cgaccgggtc cgcgaccgga atcccggtcc tcatcgacgc
    12181 caacgggcag ctcggcacgg tctcctcctc ggcatcggtc aagcacgaca tccgggacat
    12241 gggcgaactc agcgcccgcc tcatggatct gcgccccgtt gcgttccgct acaacgccca
    12301 gggcgaggac gcccccgttc acttcggcct cgtcgccgag gaggtgaacg aggtcctgcc
    12361 cgagctagtg gcccgcaaca aggccggcga gatcgagacc gtgatgtacc acgaactgcc
    12421 tgccatgctg ctgaacgagg tgaagaagct tcgtcgcgcg atcgacgcgc tcaggggtga
    12481 gatggaggct ctcaaggccg ctcgctgatc gacgtcagtc gaacggcggc ctcgtcatct
    12541 tcgaagcccg ccggccgtag ggaatctcaa cactcgcctt gggcctgcgg atcacccacg
    12601 gccgggcagg ggcttctcca acctcaggat catctgcaga ccggatagct ggccggcggc
    12661 acccccggga gggaattccc gccggctgtg caccttcggc acacgcgcac cgtcaggaaa
    12721 gtaatctgcc ccggaatgac gtgcgccgtc ggcgtgggac ctacccaggc cgtcacccat
    12781 cccgtggcat ctgtgggctt cgccctgacc tcatacgtgg tggggaagag atccgtgaat
    12841 ttcgctcggc catccaatcc cgtcttcttc tcgaaacgac ccgcagcgac ctcgatgccg
    12901 ggcacgacga catgaccatc tccggtcttc tcgcagctgc tgggagcctc cacggtcacg
    12961 acgagcgtgc cggactcctt gcggacggcc tgtggggtct ccgcttgttt ctgcgaaggt
    13021 ggttgatccg cgccgacggc gggcggggag ctcgcgagga cggcgaacgc ggcgagcagg
    13081 gagatcccca gagtcggaga gcacgttcgg gttctgctca tggtattgcc tcctgcggct
    13141 ccttctctca ggcgctcgca acccccatct cggcggttgc gtgcatcgtg cacttccgtt
    13201 caacgaagcg acggaaagca tacgcggcgg ccgtggggaa tctcaatacg cgcctcgggc
    13261 ttgcgaattc tctccggatt gctgttgaga tcgcgtctca atcccgagac aagaggagcg
    13321 aagatgacgc gatccgggcg ccacgacgcg ccgttcgcga ccaccccggt tccgatgatc
    13381 ctctggggcg ccgcctcgag cccgtacttc ctgagttcgg tcggctcggg cgcggcgagc
    13441 atgtcgtttc aggtccggaa cgcggcgggc gccgtgatcg atcccgagac gggcttctat
    13501 ttcatggccg tgcagcggtt ctgaggccag cgccgcctac tgccagtagc cggtcacgtc
    13561 gacgacgagg tgcaccgtgc cggacgggat cgtcgtcgac accgtgacgc tgcccgccgg
    13621 cgtcccgaag aggccgagca cggcgttgtt ggcgcgcgtc tgccccgcgc ggaatgcgac
    13681 ggtgtcgagc gacggcgtcg tccccgcggg accgacgcgc acggctcccg cggcggtcga
    13741 gcccgtgacc gtcacgttcg ccgcgacgga cgtcgcgccg gaaggcacgc cgcagcggcc
    13801 catgaggacg aacgtccgcg tgccgccggc ggcgagcgcc g
//
LOCUS       DAOMFY010000084        13776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1372_length_13776_cov_7.616136, whole genome shotgun sequence.
ACCESSION   DAOMFY010000084 DAOMFY010000000
VERSION     DAOMFY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1372_length_13776_cov_7.616136"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /gene="rplL"
                     /locus_tag="PLB01_08775"
     CDS             complement(<1..117)
                     /gene="rplL"
                     /locus_tag="PLB01_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012234.1"
                     /note="present in two forms; L12 is normal, while L7 is
                     aminoacylated at the N-terminal serine; the only multicopy
                     ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two
                     L12 dimers bind L10; critically important for translation
                     efficiency and fidelity; stimulates GTPase activity of
                     translation factors; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQQ77437.1"
                     /translation="MALNVETFVKEIESMSVLELNNLVKALEEKFGVSAAMMA"
     gene            complement(142..684)
                     /gene="rplJ"
                     /locus_tag="PLB01_08780"
     CDS             complement(142..684)
                     /gene="rplJ"
                     /locus_tag="PLB01_08780"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQQ77438.1"
                     /translation="MTTTRASKAEELAELAATVGKASHAFLIDYKGLSVPAVTDLRRQ
                     IRAAKSDYIVVKNTLALRALEGKPLGSLKEHFTGMTGVAYSTTDVVALAKVLHTFGKT
                     NPNVKVKAALLDGKPVAAKELETLANLPSRAELVGKLLGLMQSPIRRLVTVLAAPHRN
                     VAATLAAVAQKKAKSEETTA"
     gene            complement(686..1393)
                     /gene="rplA"
                     /locus_tag="PLB01_08785"
     CDS             complement(686..1393)
                     /gene="rplA"
                     /locus_tag="PLB01_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQQ77439.1"
                     /translation="MSGKKFTASAAKVDKAKLYDVSQAVALVKSASFAKFNETVEVAL
                     RLGVDPKHADQMVRGTVVLPHGLGKTTRVAVVASGEKIKEAEAAGADVVGGDDLVAKI
                     QAGMLDFEALVATPDMMKSLGRLGKVLGPRGLMPNPKAGTVTFDVGKAVKELKAGKIE
                     FRVDKTAIIHVPVGKVSFPENQLVENAEALLVAVQKAKPAAAKGKYMKSVVLSSTMGP
                     GVPVDPATAEKLVGRTA"
     gene            complement(1527..1952)
                     /gene="rplK"
                     /locus_tag="PLB01_08790"
     CDS             complement(1527..1952)
                     /gene="rplK"
                     /locus_tag="PLB01_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQQ77440.1"
                     /translation="MAKKVTGYVKLQIEAGKATPAPPVGTALGPQGVNIMDFCKAFNA
                     KTAKDAGLIIPVVVTVYSDRSYSFITKTPPAAVLLKKAAKLEKGSGVPNKTKVGKVTK
                     KQVEEIAKLKLVDLNTVDLAAAVKSVEGTARSMGIEVVG"
     gene            complement(2059..2586)
                     /gene="nusG"
                     /locus_tag="PLB01_08795"
     CDS             complement(2059..2586)
                     /gene="nusG"
                     /locus_tag="PLB01_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQQ77441.1"
                     /translation="MDWFIVHTYSGFEDRVVTELQNRIKALGMEVHFGEIRVPKETVV
                     EMKAGKRRNVERKFFPGYVLVQMEMSDETWHLVRNTPKVTGFVGGSSKAPVPLTQDEV
                     DSILHHTAESKEKPKPRYVYERGEQVKITDGPFKEFTGTVEDINLERSTLRVLVTIFG
                     RPTPVELEFVQVQKL"
     gene            complement(2672..2884)
                     /gene="secE"
                     /locus_tag="PLB01_08800"
     CDS             complement(2672..2884)
                     /gene="secE"
                     /locus_tag="PLB01_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQQ77442.1"
                     /translation="MNFSERWTTFRGFLSDVKKETSKVSWPDRDEVVGTTTVVIVYTV
                     LVGIFLFLVDAAVTPLVNKLFAAFGG"
     gene            complement(2962..3039)
                     /locus_tag="PLB01_08805"
     tRNA            complement(2962..3039)
                     /locus_tag="PLB01_08805"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3002..3004),aa:Trp,seq:cca)
     gene            complement(3072..3146)
                     /locus_tag="PLB01_08810"
     tRNA            complement(3072..3146)
                     /locus_tag="PLB01_08810"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3112..3114),aa:Gly,seq:tcc)
     gene            complement(3174..3249)
                     /locus_tag="PLB01_08815"
     tRNA            complement(3174..3249)
                     /locus_tag="PLB01_08815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3214..3216),aa:Thr,seq:ggt)
     gene            complement(3267..3354)
                     /locus_tag="PLB01_08820"
     tRNA            complement(3267..3354)
                     /locus_tag="PLB01_08820"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3318..3320),aa:Tyr,seq:gta)
     gene            complement(3401..3476)
                     /locus_tag="PLB01_08825"
     tRNA            complement(3401..3476)
                     /locus_tag="PLB01_08825"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3441..3443),aa:Thr,seq:tgt)
     gene            complement(3605..4348)
                     /gene="rlmB"
                     /locus_tag="PLB01_08830"
     CDS             complement(3605..4348)
                     /gene="rlmB"
                     /locus_tag="PLB01_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQQ77443.1"
                     /translation="MIVSGFHPVREVLEKRPRAVEALLVQRGRKDARAGEIEELARSA
                     GVPVRLVAKEELDRFAGRAHNGVAARVAEREYDDVEACLAGPKGGRCVLFLDEVTDPG
                     NLGSVLRTAAAAGASVVLTERHSAGLNETVSKASAGALERVKVGRVVNAARFLEQAKA
                     AGFWIYGAAMDGESLWEVDISGDAVLCLGSEGAGLRRLTRETCDRLISIPMNPGAGSL
                     NVSVAAGVLLLEWVRRKSGPPASSGMKMA"
     gene            complement(4355..4831)
                     /gene="ispF"
                     /locus_tag="PLB01_08835"
     CDS             complement(4355..4831)
                     /gene="ispF"
                     /locus_tag="PLB01_08835"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQQ77444.1"
                     /translation="MRIGHGFDAHRLEPGRRCVLGGVAVPSDSGPIGHSDGDVVLHAL
                     ADALLGAAAMGDLGTVFGTDRPEWAGAAGEAFVRHVRELTDHPAILNVDVTILAVRPR
                     VGEYREAMRENIAALLGIPVARVSVKASSGNGLTDFGRGEGVAATVVLLAGGDAAE"
     gene            complement(4836..5528)
                     /gene="ispD"
                     /locus_tag="PLB01_08840"
     CDS             complement(4836..5528)
                     /gene="ispD"
                     /locus_tag="PLB01_08840"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQQ77445.1"
                     /translation="MSQATALVPAAGFGTRFHEGGPKALVLLAGRPLLVHALERLAAS
                     KRVSSAVVAIPGGCEADFREALKASPLPYVLVEGGVTRRESVARAFAAADPKDDDALV
                     LVHDAARPLVDPLEVKAVVDAADAKGGAIAAFSLVETIKRVESGRIKATVPRGDLVAA
                     TTPQAFRAGVLRKGLAKEGGPDATDDAELVERTGGEVTVVLTSRWNIKITYPEDLAWA
                     EAWLAHCGHVGA"
     gene            complement(5525..6088)
                     /gene="frr"
                     /locus_tag="PLB01_08845"
     CDS             complement(5525..6088)
                     /gene="frr"
                     /locus_tag="PLB01_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQQ77446.1"
                     /translation="MPTIAETKKETERRMHASIEALKHELKHLRTGRASVGILEGIFV
                     EYYGTATPLNQVANLSAPDATLLLASPWEPSLCPAIEKAIRTSDLGLNPSSDGKVVRI
                     PVPQPTEERRKEIIKKAHGFAEHAKVEIRHHRHEANDAIKKEAKASAVSADDEKRALD
                     EIQKLTDKSVAEVDHILKTKEAEVLAR"
     gene            complement(6119..6841)
                     /gene="pyrH"
                     /locus_tag="PLB01_08850"
     CDS             complement(6119..6841)
                     /gene="pyrH"
                     /locus_tag="PLB01_08850"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQQ77447.1"
                     /translation="MEPRYRRVLLKLSGEALLGNQPFGIEPAVVARIADEVAEVARLG
                     VETAIVIGGGNIIRGVSAATQGIDRVTGDNMGMLATVINALALQDAMEKREIHTRVMT
                     AFEVRAVAEPFIRRRAIRHLEKKRCVIFAGGTGNPYFTTDSAAALRANEIRADAILKA
                     TRVDGIYSDDPKKNPNAEFLPRVSYQDVLEKRLAVMDATSVALCRDNRMPIRVFNLLV
                     PGNIRRVVLGEPVGSVVDAGRA"
     gene            complement(6846..7766)
                     /gene="tsf"
                     /locus_tag="PLB01_08855"
     CDS             complement(6846..7766)
                     /gene="tsf"
                     /locus_tag="PLB01_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQQ77448.1"
                     /translation="MAISAVTVKMLRDKTGAGMLDCKSALEETNGNIEEAEKILRKKG
                     LAAAAKKAGRVAADGLIGYHVGSGVAALIELNCETDFVAKTEKFKQALHAITNFVHGH
                     EALGDTADGDVAALTASAPPSKMPHAGATIGECLQAFTGDLGEKTELRRFARLVAGPG
                     SSLTLYAHPGDKTVVVIETKGMPEDLGKDLAMHVAALAPQFATRDGVSAKALADEREI
                     ARAKAIAAGKPEAVAEKMVEGMIGKWYGEVVLLDQAFAKDDSKKVSQVVAERGAKDAA
                     VIRFVRYKVGEGVEKKAGGDFAAEVAALTK"
     gene            complement(7843..8646)
                     /gene="rpsB"
                     /locus_tag="PLB01_08860"
     CDS             complement(7843..8646)
                     /gene="rpsB"
                     /locus_tag="PLB01_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQQ77449.1"
                     /translation="MSQITMKELLEAGVHFGHQTKRWNPKMKKFIFGKRNGIYIIDLQ
                     KTLKCFREAAAFITDVASQGKNILFVGTKRQAQDAIYEEANRCGMFYVNNRWLGGTLT
                     NFVTIRKSISRLKEIEAMLVEGSDAILTKKERIQLDREKAKLAKNLVGIKEMEELPDV
                     LFVIDPKKEAIAILEAKKLGIPVVAIVDTNCDPDDISFVIPGNDDAIRAIKLFTGKIA
                     DAVLEGLHAQEERFVGKVVHGEDEPMPTNFQVTEAGAAKPEGPAVPVQA"
     gene            complement(8657..9049)
                     /gene="rpsI"
                     /locus_tag="PLB01_08865"
     CDS             complement(8657..9049)
                     /gene="rpsI"
                     /locus_tag="PLB01_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQQ77450.1"
                     /translation="MTNLQFQAVGRRKESVARVFLRPGKGAVQVNDRTFEDYFPNDVL
                     KMVIRQPLQLTETVEKFDVVALVEGGGSAGQAGAIRHGIARALCLFNGELRGALKKAG
                     LLTRDSRMKERKKYGQRGARARFQFSKR"
     gene            complement(9075..9509)
                     /gene="rplM"
                     /locus_tag="PLB01_08870"
     CDS             complement(9075..9509)
                     /gene="rplM"
                     /locus_tag="PLB01_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQQ77451.1"
                     /translation="MSTRTPLPKISDHTRRWIVVDADGKVLGRLATLVARHLTGKTKA
                     IYTPFLDTGDFVVVVNAEKVALTGAKSQSKLYRHHTGFPGGVKTVEFDKLRATKPERV
                     IEEAVKGMLPKTKLGRKMFKKLKVYAGPHHPHTAQAPAAVAV"
     gene            9728..10645
                     /locus_tag="PLB01_08875"
     CDS             9728..10645
                     /locus_tag="PLB01_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HQQ77452.1"
                     /translation="MQPRILDGAAVAKALREEAAAGAARLVTRGIVPGLTVVLVGEDA
                     ASSTYVRNKEIAAREAGFAGRTLRFPASLPEADLLATIDSLNRDDAVDGILVQLPLPK
                     HIATARVLEAIDPRKDADGFHPENAGRLLQGKPAPVPCTPAGILELLRREGVTLRGTR
                     AVVVGRSDIVGKPMALLLLQNDATVTVAHSKTRDLPALCREADLLVAAIGRPGFVTGD
                     FVKEGAVVVDVGINRVTTRADLERFFPGDAKRLAAFEKNGSTLVGDCDPATAFPRASR
                     YTPVPGGVGPLTIAHLLLNTLRLCEARRG"
     gene            10642..11292
                     /gene="coaE"
                     /locus_tag="PLB01_08880"
     CDS             10642..11292
                     /gene="coaE"
                     /locus_tag="PLB01_08880"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQQ77453.1"
                     /translation="MILKVGLTGGLASGKSTIAEGLEKRGIPVRDADRMVHELYAPGA
                     AGARAVAAAFGPEFLAADGSVDRPKLSMHVFHDAEQLARLNGLIHPLVREAQAKWFEE
                     LAARGEPLGVIEATLLVETGGRERFDVLVAVSAPADMRLQRAVRRSGETDPNEFVKRM
                     SAQFSDAAREEVSDIVIRTDGTREELEDKIDRLADELRVRAADAGAGRRSGFFSRP"
     gene            complement(11279..11851)
                     /gene="rdgB"
                     /locus_tag="PLB01_08885"
     CDS             complement(11279..11851)
                     /gene="rdgB"
                     /locus_tag="PLB01_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQQ77454.1"
                     /translation="MTDALAGLVFVTSNPGKAREASVLLGRAVEARALDLPEIQSLDF
                     REVARAKAIVAAHALGVPVLVEDSGLAVAGWGGFPGPLTKFITMGVLGQEGLAKMLDG
                     FSDRAAEAVSVLAVARPGQREPDVIVAEGRVKGSIALHPRGENGFGWDVLFIPEGETR
                     TFAEMGDDEKNAVSHRARAFAALRAALTAG"
     gene            complement(11848..13476)
                     /locus_tag="PLB01_08890"
     CDS             complement(11848..13476)
                     /locus_tag="PLB01_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQQ77455.1"
                     /translation="MPLMELVYEVEGQTRRYLLSGNDVSIGRSSENGIVLNDFSVSRK
                     HALLSESGGVWTIEDLKSTNGLKVNGEFTPAARIKPGDVLTVGTFTLTVNEQPTFRGR
                     ATASISDSSATFVRSIADFNRDFGFDAAPLPKEDVLAGTMAGRRPATREKVFEAMVLV
                     ARTLIEAREVGQILSKVVDLLFDFLPAERAVVVLLEKDGRPTPTLARRRGREGSDPEA
                     SFSHTIVESVVRDRVAVLTSDALADDRFEAGQSIRIQQIRSAMCVPLWDRDRVIGALH
                     VDTPLKAGTFTADDLDLLTALGNFAAVAIERARLQGRIEREERIRERLSRYHSPGVVD
                     EIVTGGPQGVESVRTREVTVFFADIVGFTSSAETMDPEEVSRFLERVFTFAADAIFEQ
                     GGTLDKFIGDAVMAFFGAPIPQPDHARRAVAAAAKLIESLHAWNLQRAADGEPSVAVR
                     VGVNTGRAFVGDIGSDRRVDYTVLGNTVNVAARLESTVAGPNEVVVGVETARQAGGTY
                     EFEPLGEQKLKGLSKGMTAFRLKLDASGRPVTTP"
     gene            complement(13505..>13776)
                     /locus_tag="PLB01_08895"
     CDS             complement(13505..>13776)
                     /locus_tag="PLB01_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQQ77456.1"
                     /translation="TRVDPQYPEAAKRMKLGGDVVLRVVVEANGTIGRIETVTGAPFG
                     MTEAATDAVRKWTYRPARVNGQAVAVWKVIRVKFALHAEREPPPD"
BASE COUNT         1995 a         4738 c         4673 g         2370 t
ORIGIN      
        1 ggccatcatc gcggcggaga ccccgaactt ctcctcgaga gccttgacga gattgttcag
       61 ctcgaggacg gacatcgact cgatttcctt gacgaaagtc tcgacgttga gagccatggt
      121 tctatctcct gattcttcgg cttaagccgt ggtttcttcg gactttgctt ttttctgggc
      181 cacggccgcg agggtcgcgg cgacgttgcg gtgaggggcg gcgaggaccg tcacgaggcg
      241 ccggatcggc gactgcatga ggccgaggag cttgccgacg agctcggcgc gcgagggcag
      301 gttcgcgagg gtctcgagct ccttcgcggc gacgggcttg ccgtcgagga gcgcggcctt
      361 gaccttcacg ttcgggttcg tcttgccgaa ggtgtggagg accttcgcga gcgcgacgac
      421 gtccgtcgtc gagtacgcga cgcccgtcat ccccgtgaag tgctccttga gcgagccgag
      481 gggcttgccc tcgagggccc gcagggcgag cgtgttcttc acgacgatgt agtcggactt
      541 cgcggcgcgg atctggcggc gcaggtccgt cacggccggc accgaaaggc ccttgtagtc
      601 gatcaggaac gcgtgggaag ccttccccac ggtcgccgcg agctcggcga gctcctcggc
      661 cttggaggcg cgtgtggtcg tcatgtcagg ccgtccttcc cacgagcttc tcggcggtcg
      721 cgggatccac cgggacgccc ggtcccatcg tcgaggagag gacgacggac ttcatgtact
      781 tgcccttcgc ggcggcgggc ttggcttttt gaacggcgac gagcagagcc tcggcgttct
      841 cgacgagctg gttctcgggg aacgagacct tgccgacggg gacgtggatg atcgccgtct
      901 tgtcgacgcg gaactcgatc ttgccggcct tgagctcctt gacggccttg ccgacgtcga
      961 acgtgaccgt ccccgccttc gggttcggca tgaggccgcg cgggccgagc actttgccga
     1021 gccgtccgag cgacttcatc atgtcgggcg tcgcgacgag ggcctcgaag tcgagcatgc
     1081 cggcctggat cttcgcgacg aggtcgtcgc cgcccacgac gtccgctccc gccgcttcgg
     1141 cttccttgat cttctcgccc gaggccacga cggccacgcg ggtcgtcttg ccgaggccgt
     1201 gcggaagcac gaccgtgccg cggaccatct ggtccgcgtg cttcgggtcg acgccgaggc
     1261 ggagggccac ctcgaccgtc tcgttgaact tggcgaacga cgcggacttg acgagcgcga
     1321 cggcctggga cacgtcgtag agcttcgcct tgtcgacttt cgcggcggac gcggtgaatt
     1381 tcttgccact catggtgtgt tgttctccgg agtcggtcgc atcccccggg tccgtcggtg
     1441 agaagccccg gggcatcccg ggcaccggcg aaccgcgcgc cgggagaggc ccggcgcggg
     1501 aggcttccgc ctcgttgatc gcggcgtcag ccgacgacct cgattcccat ggatcgggcg
     1561 gtcccctcca ccgacttgac ggcggcggcg aggtccacgg tgttcaggtc gacgagcttg
     1621 agcttggcga tctcctcgac ctgcttcttc gtcaccttgc cgaccttcgt cttgttcggg
     1681 acgccggagc ccttctcgag cttggcggcc ttcttgagca ggacggcggc gggaggcgtc
     1741 ttcgtgatga acgaatacga ccggtccgaa tacaccgtga cgacgacggg gatgatgagc
     1801 ccggcgtcct tcgcggtctt cgcgttgaag gccttgcaga agtccatgat gttgacgccc
     1861 tgcggaccga gcgcggtgcc gacgggcgga gccggggtgg ccttccccgc ctcgatctgg
     1921 agcttcacgt atccggtgac tttctttgcc atggtttctg ccttctcgct tcaggtttcg
     1981 gatgaagatt tctcggaggg tgaagagaga atttcgtctc tcttactttc gaagaaatcc
     2041 gaaaaacaaa acgatctcct agagtttctg tacctgaacg aactccagtt ccaccggagt
     2101 cggtcttccg aagatggtga cgaggacgcg gagcgtcgag cgctcgaggt tgatgtcctc
     2161 gaccgtgccc gtgaactcct tgaaggggcc gtccgtgatc ttgacctgct cgccgcgctc
     2221 gtagacgtaa cgcggcttcg gcttttcctt ggactcggcc gtgtggtgga ggatcgagtc
     2281 gacctcgtcc tgcgtgagcg ggaccggcgc cttcgacgat ccgccgacga agcccgtgac
     2341 cttcggcgtg ttgcgcacga ggtgccaggt ctcgtcggac atctccatct gcacgaggac
     2401 gtagcccggg aagaacttcc gctcgacgtt gcggcgcttc ccggccttca tctcgacgac
     2461 cgtctccttc ggcactcgga tctctccgaa atggacctcc atgccgaggg ccttgatgcg
     2521 gttctgcagc tccgtcacga cacggtcttc gaagcccgag tacgtgtgga cgatgaacca
     2581 gtccatgtgc ggcgccgccg cagccggggc cgcgctcgcc tgttcctggg ggtcgacttc
     2641 gctcaccgct gtgccttcat caattccgtc ttcagccgcc gaaggcggcg aagagcttgt
     2701 tcacgagcgg cgtgaccgcc gcgtcgacga gaaagaggaa gatgcccacg aggaccgtgt
     2761 agacgatgac caccgtcgtc gtcccgacga cctcgtcccg gtccggccac gacaccttcg
     2821 aggtctcctt cttcacgtcc gaaaggaagc cgcggaaggt cgtccaacgc tcgctgaagt
     2881 tcatgtcgtt tcgttcgtcc ttcgtgtcga ttcgggtccg tcgtttcgtg tctcgtgtct
     2941 cgtcgtggat caggccgagg ttggcagggg tgcggggatt cgaaccccga acctacggtt
     3001 ttggagaccg ccgctctagc cagttggagc tacacccctg tatgtcgtcg tcgcgtggca
     3061 agctgcatga atggagcggg agacgggatt cgaacccgcg accaacagct tggaaggctg
     3121 tgactctacc cctgagttac tcccgctcag gaacgtgatc ggcgcgggga aactggagcc
     3181 cacgagcaga tttgaactgc tgaccccgtc cttaccaagg acgtgctcta ccgactgagc
     3241 tacgtgggcc cccttctcta caaagatggt gggcagagag ggattcgaac ccccgtagca
     3301 cgtcagtgcg gcagatttac agtctgctgc cattaaccac tcggccatct gcccgttccc
     3361 gtccttcatg attcccgtgt ccggcccccg gagctctccg tggagctggc gatgggactt
     3421 gaacccgcaa cctgccgatt acaaatcggc tgctctgcca attgagctac gccagcactc
     3481 gctttcctcc ccagagagag ctcgaccgtg cgacccgatg accctggaga cacggagaac
     3541 gcacggcttc gcgcacccgg cggaggcgcg aactgtggtt tatatcagcc ggcctgaaac
     3601 cccgtcaagc cattttcatg ccggaacttg ccggcgggcc gcttttcctc ctgacccact
     3661 cgagaagcag gactccggcc gccaccgaga cgttcaggga cccggctccg gggttcatgg
     3721 ggatcgaaat caagcgatcg cacgtttctc gggtcagacg ccggagtccc gctccttcag
     3781 agcccaggca gaggacggcg tcaccggaaa tatcgacttc ccagagactt tcgccgtcca
     3841 tcgccgcccc gtagatccag aatccggccg ccttcgcctg ctcgagaaag cgggcggcgt
     3901 tgaccacccg gcccactttg acccgctcga gggcgcccgc cgaggccttg gagaccgttt
     3961 cgttcagccc ggccgagtgc cgctcggtca ggacgaccga ggcccccgcg gccgccgccg
     4021 tgcggagaac cgacccgagg ttgcccgggt ccgtcacctc gtcgaggaac aggacgcacc
     4081 gtccgccctt cggacccgcg agacacgcct ccacgtcgtc gtactcgcgc tcggccaccc
     4141 gggccgccac gccgttgtgc gcgcggcccg cgaatcggtc cagctcctcc ttcgcgacga
     4201 ggcgcacggg cacgcccgcg ctccgcgcga gctcctcgat ctcccccgcc cgggcgtcct
     4261 tgcggccgcg ctggacgagg agcgcctcga ccgcgcgcgg ccgcttctcg aggacctcgc
     4321 gcaccgggtg gaagcccgag acgatcacgg cccgctactc ggccgcgtcg ccgccggcga
     4381 ggaggaccac ggtcgccgcc acgccctcgc cccggccgaa gtccgtcaac ccgttcccgg
     4441 acgaggcctt cacggagacg cgcgcaacgg ggatcccgag gagggccgcg atgttctcgc
     4501 gcatggcctc gcggtattcc cccacgcggg gcctcacggc caggatcgtg acgtccacgt
     4561 tgaggatcgc cggatggtcc gtcagctcgc ggacgtgccg gacgaaggcc tcccccgcgg
     4621 cgcccgccca ctcgggtcgg tcggtcccga aaacggtccc gagatcgccc atcgcagcgg
     4681 cgccgagcag cgcgtccgcg agcgcgtgca ggacgacgtc cccgtccgag tgtccgatcg
     4741 ggccggagtc cgacggcacc gcgacgccgc cgagcacgca tctccgcccg ggctcgagcc
     4801 ggtgggcgtc gaatccgtgc ccgatcctca cggggtcacg ctcccacgtg gccgcaatgg
     4861 gccagccagg cctccgccca cgcgaggtcc tcggggtacg tgatcttgat gttccagcgc
     4921 gacgtcagga cgaccgtgac ctcgccgccg gtgcgttcga cgagctccgc gtcgtccgtc
     4981 gcgtcggggc cgccttcctt cgcgagcccc ttgcgcagga ctcccgcgcg gaaggcctgc
     5041 ggggtcgttg ccgcgacgag atctccgcgc gggaccgtgg ccttgatccg gcccgattcg
     5101 acgcgcttga tcgtctcgac gagggaaaac gcggcgatcg cgcccccctt ggcgtccgcc
     5161 gcgtccacga cggccttgac ctcgagcggg tcgacgagcg ggcgggccgc gtcgtgcacg
     5221 aggacgagcg cgtcgtcgtc cttggggtcg gcggctgcaa acgcccgcgc gacggactcg
     5281 cgtcgggtga cgccgccctc gacgagaacg tacggaaggg gcgacgcctt cagcgcctcc
     5341 cggaagtccg cctcgcagcc gcccgggatc gcgacgacgg ccgaggacac ccgcttgctc
     5401 gccgcgaggc gctcgagcgc gtggacgaga agcggccggc ccgcgaggag gacgagagcc
     5461 ttcggcccgc cctcgtggaa gcgggtaccg aagccggcgg ccgggacgag agccgtggcc
     5521 tggctcaccg tgcgagaact tccgcttcct tcgtcttgag gatgtggtcg acctcggcga
     5581 cgctcttgtc cgtgagcttc tggatctcgt cgagggcgcg tttctcgtcg tccgccgaaa
     5641 cagcggaggc cttcgcctcc ttcttgatgg cgtcgttcgc ctcgtggcgg tggtggcgga
     5701 tctcgacctt cgcgtgctcg gcgaagccgt gagccttctt gatgatctcc ttgcgccgct
     5761 cctccgtggg ctgcggcacg ggaatcctga cgacctttcc gtccgacgac gggttgagcc
     5821 cgaggtcgct cgttcggatc gccttctcga tggccgggca gagcgacggc tcccagggag
     5881 acgcgaggag cagagtggca tccggcgcgg agaggttcgc gacctggttg agcggcgtcg
     5941 ccgtcccgta gtattccacg aagatccctt cgaggatccc caccgaggcc cgcccggtgc
     6001 gcaggtgctt gagctcgtgc ttgagggcct cgatcgaggc gtgcatccgc ctctcggttt
     6061 ccttcttcgt ctcggcgatc gtgggcatgg cgtactcctt gttcgggcgg gcgcggcgtc
     6121 aggcgcgccc tgcgtcgacc acgcttccga ccggctcgcc caggacgacg cggcggatgt
     6181 tcccggggac gagcaggttg aacacgcgga tcggcatgcg attgtcgcgg cagagggcga
     6241 ccgacgtcgc gtccatgacg gcgagccgct tctcgaggac gtcctggtag ctgacacgcg
     6301 ggaggaattc ggcgttcggg ttcttcttcg gatcgtccga gtagatgccg tcgacgcgcg
     6361 tcgccttgag aatcgcgtcg gcgcggatct cgttggcgcg gagggccgcc gccgagtccg
     6421 tcgtgaagta cgggttgccc gtgccgcccg cgaagatgac gcagcgtttc ttttcgagat
     6481 ggcggatcgc gcggcgccgg atgaacggct cggcgacggc gcgcacctcg aacgccgtca
     6541 tgacgcgggt atggatctcg cgcttctcca tcgcgtcctg gagcgcgagg gcgttgatga
     6601 cggtcgcgag catccccatg ttgtcgcccg tgacgcggtc gatgccctgc gtggcggccg
     6661 acactccccg gatgatgttc ccgcccccga tcacgatcgc cgtctcgacg ccgagtcgcg
     6721 cgacctcggc gacctcgtcc gcgatgcggg cgacgaccgc cggctcgatg ccgaagggct
     6781 ggttgccgag gagcgcctcc ccggagagct tcagcaggac ccggcggtag cggggttcca
     6841 cggcgttact tcgtcagcgc ggcgacttcg gcggcgaaat cgcccccggc cttcttctcg
     6901 acgccctcgc ccaccttgta gcggacgaaa cgaatcacgg ccgcgtcctt ggcgccgcgc
     6961 tcggcgacga cctgggagac ctttttcgag tcgtccttcg cgaacgcctg gtcgaggagg
     7021 acgacctcgc cgtaccactt gccgatcatt ccctcgacca tcttctcggc gacggcctcg
     7081 ggcttgcccg cggcgatcgc cttggcgcgc gcgatctccc tctcgtcggc gagagccttg
     7141 gcggaaacgc cgtcccgggt cgcgaactgc ggcgcgagcg cggcgacgtg catcgcgagg
     7201 tcctttccga ggtcctcggg catgcccttc gtctcgatca cgacgaccgt cttgtcgccc
     7261 gggtgcgcgt agagcgtgag cgacgagccg gggcccgcga cgaggcgcgc gaagcggcgc
     7321 agctcggtct tctcgccgag gtcgccggtg aacgcctgga ggcactcccc gatcgtggcg
     7381 ccggcgtggg gcatcttcga gggcggagcc gacgccgtca gcgcggcgac gtctccgtcg
     7441 gcggtgtcgc cgagggcctc gtggccgtgc acgaagttcg tgatcgcgtg aagcgcctgc
     7501 ttgaacttct ccgtcttcgc gacgaagtcg gtctcgcagt tcagctcgat cagggccgcg
     7561 acgccgctgc ccacgtggta gccgatgagc ccgtcggccg cgacgcgccc ggccttcttg
     7621 gccgcggcgg cgaggccctt cttgcggagg atcttctcgg cctcctcgat attgccgttc
     7681 gtctcttcga gcgcgctctt gcaatcgagc atgccggcgc ccgtcttgtc ccggagcatc
     7741 ttgaccgtca ctgcggaaat cgccatggag tcctctctga catcaaaaag gccggcgggg
     7801 gaccccaagt cctcccgccg gcccgaatcg cgtggatcgg gatcaggcct ggacggggac
     7861 ggcggggcct tcgggcttgg ccgcgcccgc ctcggtcacc tggaagttcg tgggcatcgg
     7921 ctcgtcctcg ccgtggacca ccttcccgac gaagcgctct tcctgcgcgt gcaggccctc
     7981 gagcaccgcg tcggcgatct tgcccgtgaa gagcttgatc gcccggatgg cgtcgtcgtt
     8041 gccggggatc acgaagctga tgtcgtcggg gtcgcagttc gtgtcgacga tggcgacgac
     8101 cgggatgccg agcttcttgg cctcgaggat cgcgatcgcc tccttcttgg ggtcgatcac
     8161 gaacaggacg tccggaagct cctccatctc cttgatgccg acgaggttct tggcgagctt
     8221 cgccttctcg cggtcgagct ggatgcgctc cttcttggtg aggatcgcgt ccgagccctc
     8281 cacgagcatg gcctcgatct ccttgaggcg cgagatcgac ttgcggatcg tcacgaagtt
     8341 cgtgagcgtg ccgccgagcc agcggttgtt cacgtagaac atgccgcagc ggttggcttc
     8401 ctcgtagatc gcgtcctgcg cctggcgctt cgtccccacg aagaggatgt tcttgccctg
     8461 cgacgcgacg tccgtgatga aggccgcggc ctcgcggaag cacttcagcg tcttctggag
     8521 gtcgatgatg tagatcccgt tgcgcttgcc gaagatgaac ttcttcatct tcgggttcca
     8581 gcgcttcgtc tggtggccga agtgaacgcc cgcctcgagc agctccttca tcgtgatctg
     8641 gctcattcgt cccccattac ctcttcgaga actggaaccg ggcgcgggcg ccgcgctggc
     8701 cgtacttctt gcgctccttc atgcgcgagt cgcgcgtgag gaggcctgcc ttcttcaggg
     8761 cgccgcggag ctcgccgttg aacaggcaga gcgcgcgggc gataccgtga cggatcgcgc
     8821 cggcctggcc cgcgctcccg ccgccctcga cgagggcgac gacgtcgaac ttctcgaccg
     8881 tctcggtgag ctgcaggggc tggcggatga ccatcttgag cacgtcgttc gggaagtagt
     8941 cttcgaaggt gcggtcgttg acctggaccg cgcccttccc ggggcggagg aacacgcggg
     9001 cgacggattc cttgcggcgt ccgacggcct gaaactggag gttcgtcacg gggtgcgctt
     9061 ctccttcgtc ccggtcagac cgcgacggcc gcgggggcct gggcggtgtg cgggtggtgc
     9121 ggacccgcgt agaccttgag cttcttgaac atcttccggc cgagcttcgt cttcgggagc
     9181 atgcccttga cggcttcttc aatgacgcgc tcgggcttcg tggcccggag cttgtcgaac
     9241 tcgaccgtct tcaccccgcc cgggaaaccc gtgtggtggc ggtagagctt cgactgcgac
     9301 ttggcgccgg tcaaggcgac cttctcggcg ttcacgacga cgacgaagtc gccggtgtcg
     9361 aggaacggcg tgtagatggc cttggtcttg cccgtgaggt gccgtgcgac gagcgtcgcg
     9421 aggcggccga ggaccttccc gtcggcgtcg acgacgatcc agcggcgggt gtggtcggaa
     9481 attttcggga gcggcgtgcg agtgctcacg ggaacggtcg tctccggcct gggtggttgt
     9541 ggggtgtttc gaccccgttg gaagatgcgg ggtcgggctc cgatgcggat ccggggaagc
     9601 atcgaaagac cagggcccct acggcacacg actcgcggga ttcaggcagg aacccgagac
     9661 ccgcggggca gtggaaaata cccccccatg acgcccctgt caagtaactc tcggtcacaa
     9721 taggaccatg cagccacgaa tcctcgacgg cgccgccgtc gcaaaggccc tccgggagga
     9781 ggccgcggca ggcgcggccc gcctcgtgac ccgaggcatc gtcccggggc tcacggtcgt
     9841 cctcgtgggc gaggacgccg cgagctcgac ctacgtccgg aacaaggaaa tcgccgcgcg
     9901 ggaggccgga ttcgcgggcc ggacgcttcg cttcccggcc tcgctccccg aggccgactt
     9961 gctcgcgacg atcgactcgc tcaaccggga cgacgcggtg gacggcatcc tcgtgcagct
    10021 tccgctgccg aagcacatcg cgaccgcccg cgtcctcgag gcgatcgacc cgcggaagga
    10081 cgccgacggc ttccaccccg agaatgccgg ccgcctcctg cagggcaagc ccgcacccgt
    10141 cccgtgcacg cctgccggca ttctcgagct cctccggcgc gagggcgtga cgcttcgcgg
    10201 aacacgcgcc gtcgtcgtcg gccggagcga catcgtggga aagcccatgg ccctcctcct
    10261 cctccagaac gacgcgaccg tcaccgtcgc gcactccaag actcgggacc tccccgccct
    10321 ctgtcgggag gcggacctcc tcgtcgcggc gatcgggcgg ccgggcttcg tgaccggcga
    10381 cttcgtgaag gagggcgcgg tcgtcgtgga cgtgggcatc aaccgcgtga cgacgcgcgc
    10441 ggatctcgag cgcttcttcc cgggcgacgc gaagcggctc gcggcgttcg agaagaacgg
    10501 ctcgacgctc gtcggcgact gcgaccccgc gacggcgttc ccgagggcct cgcgctacac
    10561 gcccgtcccg ggcggagtcg gccccctgac gattgcccac ctcctcctca acacgctccg
    10621 gctctgcgag gcgcgacgcg gatgatcctc aaggtcgggc tcacgggcgg cctcgcgtcg
    10681 ggcaagagca cgattgccga gggcctcgag aagcgcggga tccccgtgcg cgacgcggac
    10741 cggatggtcc acgagctcta cgctccgggc gccgcgggcg cgcgggccgt cgccgcggcg
    10801 ttcggtcccg agttcctcgc ggccgacggc tccgtcgacc gcccgaagct ctcgatgcac
    10861 gtgttccacg acgccgagca gttggcgagg ctgaacgggc tcattcaccc gctcgtccgc
    10921 gaggcgcagg cgaagtggtt cgaggagctc gccgcgagag gcgagccgct cggcgtcatc
    10981 gaggcgacgc tcctcgtcga gaccggcggg cgcgagcgct tcgacgtcct cgtcgcggtc
    11041 tcggcgccgg ccgacatgcg cctccagcgc gcggtcaggc gcagcggcga gacggacccg
    11101 aacgagttcg tgaagcggat gtcggcgcag ttctccgacg cggcgcgcga ggaggtctcc
    11161 gacatcgtga tccggacgga cggcacgcgc gaggaactcg aggacaagat cgaccggctc
    11221 gccgacgaac tgcgcgtccg cgcggcggac gcgggcgccg gccgccgcag cgggttcttc
    11281 agccggccgt gagcgcggcg cgcaaggccg cgaacgcccg ggcgcggtga gacaccgcgt
    11341 tcttctcgtc gtcgcccatc tccgcgaacg tgcgcgtctc cccctcgggg atgaacagca
    11401 cgtcccagcc gaagccgttc tcgccgcggg ggtggagcgc gatcgagccc ttcacgcgcc
    11461 cctccgcgac gatcacgtcc ggctcgcgct gaccgggcct cgcgacggcg agaacggaca
    11521 ccgcctccgc ggcgcggtcc gagaacccgt ccagcatctt cgcgaggcct tcctgcccga
    11581 ggacacccat cgtgatgaac ttcgtgagcg ggcccgggaa ccctccccag cccgcgaccg
    11641 cgagccccga gtcctcgacg aggacgggaa cgccgagcgc gtgcgccgcg acgatcgcct
    11701 ttgcccgcgc gacctcgcgg aagtcgaggg actggatctc gggcaggtcc agcgcccgcg
    11761 cctcgacggc gcggccgagg aggaccgagg cctcgcgcgc cttgccgggg ttcgacgtaa
    11821 cgaagaccag gccggcgagc gcgtcggtca cggggtcgtg acggggcgcc ccgaggcgtc
    11881 gagcttcagg cgaaacgccg tcattccctt cgacaggcct ttgagcttct gctcgccgag
    11941 gggctcgaac tcgtaggtcc cgccagcctg ccgcgccgtc tcgacgccga cgacgacctc
    12001 gttcggccct gcgaccgtcg actcgaggcg cgccgcgacg ttgaccgtgt tgccgagcac
    12061 cgtgtagtcg acgcggcggt cggacccgat gtcgcccacg aacgcccgcc ccgtgttcac
    12121 gccgacgcgc accgcgacgg acggttcccc gtccgcggcg cgctgcaggt tccaggcgtg
    12181 gagactctcg atgagctttg cggccgcggc gacggcgcgg cgcgcgtggt cgggctgcgg
    12241 gatcggcgcg ccgaagaacg ccatgacggc atcgccgatg aacttgtcga gcgtcccccc
    12301 ctgctcgaag atcgcgtccg ccgcaaacgt gaagacacgc tcgagaaagc gcgagacctc
    12361 ttcggggtcc atcgtctcgg ccgaagacgt gaagccgacg atgtccgcga agaagacggt
    12421 gacctcgcgt gtccgcacgc tctcgacgcc ctgcggccct cccgtcacga tctcgtcgac
    12481 gacgcccggc gagtggtacc gcgacagccg ctcgcggatg cgctcctcgc gctcgatccg
    12541 gccctggagg cgcgcgcgct cgatcgcgac ggccgcgaag ttgccgaggg cggtcaggag
    12601 gtcgaggtcg tcggccgtga acgttcccgc cttcagaggc gtgtccacgt ggagcgcgcc
    12661 gatcacgcgg tcgcggtccc agagcggcac gcacatcgcc gagcggatct gctggatgcg
    12721 gatggactgg ccggcttcga agcggtcgtc ggcgagcgcg tcggaggtga gcacggccac
    12781 gcggtcgcgc acgacggact cgacgatcgt gtgcgagaac gaggcctcgg gatcggaccc
    12841 ctccctcccg cggcgccgcg caagcgtcgg agtgggcctc ccgtccttct cgagcaggac
    12901 gacgacggcg cgttcggccg gaaggaagtc gaagagaagg tcgacgacct tcgacaggat
    12961 ctgtccgacc tcgcgcgcct cgatgagcgt gcgggcgacc aggaccatcg cctcgaagac
    13021 cttctcgcgc gtcgcgggcc ggcgccccgc catcgtcccc gcgagcacgt cctccttcgg
    13081 gagaggggcc gcgtcgaatc cgaagtcgcg gttgaagtcc gcgatggacc gcacgaacgt
    13141 cgcgctcgag tcgctgatcg acgccgtcgc gcgcccgcgg aacgtcggct gctcgttcac
    13201 cgtgagcgtg aacgtcccca cggtgagcac gtcgccgggc ttgatccggg ccgccggcgt
    13261 gaactcgccg ttgaccttca ggccgttcgt ggacttcagg tcctcgatcg tccagacgcc
    13321 gccgctctcg gacaggagcg cgtgcttgcg cgacaccgag aaatcgttga ggacgatccc
    13381 gttttcactc gatcggccga tcgacacgtc gtttcccgac agcaggtacc gtcgggtctg
    13441 accttcgacc tcgtagacga gctccatcaa cggcatgaag agccgcgatt ataggtgcgt
    13501 ccggtcagtc gggaggcggc tcgcgctcgg cgtgaagcgc aaacttgacg cggatcacct
    13561 tccagacggc gacggcctgt ccgttgacgc gcgcggggcg gtacgtccac ttccgcacgg
    13621 cgtccgtcgc cgcctccgtc atcccgaacg gggctccggt cacggtctcg atccggccga
    13681 tcgtgccgtt cgcctccacg acgacgcgca ggacgacgtc gccgccgagc ttcatgcgtt
    13741 tggcggcctc ggggtactgc gggtcgacgc gcgtga
//
LOCUS       DAOMFY010000085        13770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1374_length_13770_cov_9.691797, whole genome shotgun sequence.
ACCESSION   DAOMFY010000085 DAOMFY010000000
VERSION     DAOMFY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1374_length_13770_cov_9.691797"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PLB01_08900"
     CDS             <1..1111
                     /locus_tag="PLB01_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77457.1"
                     /translation="AQAAYWSGDYRSARRDFARVLALRPDSPVARKSLDEIASASRAR
                     WEVSAAGLSDDQPYRTAGLAARVSVFSDPLTRWDFSAGGQVLRAPDAADDRTDVPWGR
                     VGVETVFPSLRLTAAGWLEARRAPDATTLGLWSVSLRRALGSAGALSFVADRREIFAT
                     RASLADHPNVTRAGLAWSWESRAHASAGAEAFLLRYFDGNRGVAASAWALFPVFTAGA
                     FRLNAGPAVSYRDTDETRFVVAGTASTPDAGFFRYAYTGAYVPYWTPLALAEGRVAAV
                     LEGPIASGLTARFSGDAGWAHDDAVGFGPDSGLSPAPGATTSFTYRRTFHPWRASVTL
                     GLDLGGGSRLEAGYRHETTVYYRSDALHASVVGRF"
     gene            1086..2642
                     /locus_tag="PLB01_08905"
     CDS             1086..2642
                     /locus_tag="PLB01_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase catalytic subunit"
                     /protein_id="HQQ77458.1"
                     /translation="MHPWSDASEIFRHRRAKPVETWERWVLGVLLFLGLQSVAFLAWW
                     WFRLEHVHDPVLFVLLSLATWYGIFRMLVGWYNAFHVEQPPEKPAPPGLSVAIFTTSS
                     PGEPYEMFVRTLAAARDVRYPHTTYLLDDTGDPRFAALAKEMGAVPLELVGLPGAKAG
                     KINAALARTTEEFVLVLDPDHVPLPELLDRVLGHFDDPRVGFVQVAQAYHNASESFVA
                     RGAAEQTFAYYGPILQGMHGTGTAVAVGANCTFRRKALESIGGHGIGLAEDLVTSIRL
                     HAAGWTSVYVPEVVTRGLVPADLDSYLKQQLKWSRGVHEVLFHEYPKAFRRLTAFQRI
                     SYLMIGTYYLVGLTTPVYVAIPLLYLLLGLQPAAMLLSEYLLHALPVGLCGVAIYLFI
                     QRWLCDPVRERGVHGRGTLLKVGSFSVYLKGLVLAVLGVAVPYIPTAKVRQTGRFWAL
                     ARLPLFVVALSLAATAWTITRRLFVIPEAEVRISTEVTLGMLGFLVLNVVFMSGRIYA
                     AWQGRPRGTS"
     gene            2639..3811
                     /locus_tag="PLB01_08910"
     CDS             2639..3811
                     /locus_tag="PLB01_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQQ77459.1"
                     /translation="MTPRRRYRVAFFALGWLLALGVLFQFGFTSVFTRLAAKVSGALA
                     GVQQAVSDAEGRALTRARHGSRRDYLQPLAASLGSADALRDPRRVLFGAYDGAFPQSF
                     EGLEKLEQQLDFRFPLVSFYVAWGDKPDQQFPLRFVETVDRMGSVPMITWEPWVVDFD
                     AKRRTNLLSRAEREYASLAAIARGEYDFYVVPWAEAAARYGKPLFLRFAHEMNDPYRY
                     PWGPQNGNRPADFIAAWKHVHQVFQKMSATNVLWVWSPHISMPWFEYYYPGPEWVDWV
                     GFGVLNYGSVATWSRWWSFHQIVEKSYPALLALKKPLMIGEFGTLSSGGDAVEWYKQA
                     FYHLDHTYQRVRAVVFFNQSADTTMSTVPLNWSPLQDRRVSSLVAREIARKSVLEP"
     gene            3808..5439
                     /locus_tag="PLB01_08915"
     CDS             3808..5439
                     /locus_tag="PLB01_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQQ77460.1"
                     /translation="MTRFERLRPALILLAGALAAAPAAGQTVFVDGAVTISPAGSPLS
                     GITVAAYTLGGSVAGTTTSDSGGRYRLALVVGTYRLLAYDLSGVWATNFYSNATSFDT
                     SAQVNLTASLAGIDFQMARGTSVSGRVASSQGGSLSGMTVAAYNTDGSRRGFEKTNGS
                     GVFSILLPPGTYRFAAYDDNLNYATSFYLGRATYDAATPVAVSSSPVTGIDFVLSPAA
                     QVFGSTSDRATRLAIPGITANAYTTSGVLVGSATSGANGRFSMAILPGSVKFTAFDPA
                     GRYAVSFFEDAATFASAPSFPLTAGQSIGAVNFFLAPATPPSAPTTLFVPAAVNAGGA
                     GGAYFQTDLWITNPSDAALAVTVTFLPAGQDNSGRTGTPVAVAPHGQVAFTNVLQSLF
                     GASGAGALKLSASASFLATSRTYNNAAPPGTYGVGVAGRPASASVSRGLIQGLANTAA
                     FRSNVALMNPQTIPVTVTLALYRSDGTLLGQAPRTLAPLDWFQASTIFSFLGVSTIEP
                     NAYVLVGSPDGSFFAYGAVNDQTTGDGTVIEAVGY"
     gene            complement(5456..7915)
                     /locus_tag="PLB01_08920"
     CDS             complement(5456..7915)
                     /locus_tag="PLB01_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQQ77461.1"
                     /translation="MKRFSQWSLRTKLATALSAFLGVISLAIYVEVPSRLRTQAVAAT
                     LENAFTMTEMTAFDLGAALYFEDGPGLEESLSAVRRNADLVYVMVTDRSGRLAAGFNA
                     DLAERIGSRAISMAPRRLGAIPPGAPRPRSEGGFSPDDRVYQVTSPVSYNGRDIGRVT
                     VGLSLDRLNEEIAGTRRTIALVCLVIFLAGIVTVFGLSTVFTEPLGRIVAASQVIAAG
                     DLSARAPVRHDDEVGRLASAFNGMVDRLEAAQRELASRNRGLEERYRLFVERNLAGVY
                     VVTAGGRALDCNDACARLFGFDSARQFLEQGGTIATEDPADREALLVRLRREGAISNH
                     EVALRRRDGQVVWALESVRLIDGGPGEEAILEGILLDITDRKKAAQEIERRALHDELT
                     LLPNRNLFADRMGFALSLARRTQATPAVLFLDVDDLKVVNDTLGHATGDLLLRMIADR
                     LRACLREGDTVARVGGDEFTVLLPGVPGEEEAADVARKILAKVSEPYFVADDELHVTA
                     SVGVAIYPRNGETPEDLLRSADSAMYRVKVAGGNGFELAEQVPSQKALGRLSLEEELR
                     RGLERGEFVLHYQPQVNIRTNAIVGFEALVRWNHPEQVLVEPKGFISLAEYSGLIIPI
                     GEWVLHEACRQMKVWQDSGLTELNVAVNVSARQFYQRDFMGMVRRVLRKTGLMPGSLE
                     LEITESLAIQKSDWSNRILDELKEMGVGIALDDFGTGQSSLSYLRRFPIDSVKIDQSF
                     VRDLTQNANVDSIVIATLLLANRIALRTVAEGVETAGQLEFLREHGCLEMQGYLVSRA
                     LPAAEVEARFLKPLLVRRAGA"
     gene            complement(7912..8472)
                     /locus_tag="PLB01_08925"
     CDS             complement(7912..8472)
                     /locus_tag="PLB01_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HQQ77462.1"
                     /translation="MGCLSLATWAVGAALAAAPAEPVPLATQASLLLKVLAADRNLGA
                     RERTGLVIGIVYQRSSPASRDVAERFAAEVERASANDGALRARPGLIDVSAPGALAEG
                     VAALDPGALYVAPLAGFDVGRIVTLSRSLKIRTATGVPAFVEDGLAVGFESGRGAPRI
                     LINREASRAEGADFGSKLLKLARPVP"
     gene            8636..9187
                     /locus_tag="PLB01_08930"
     CDS             8636..9187
                     /locus_tag="PLB01_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQQ77463.1"
                     /translation="MQIETGTPTSTDPAGRAWAKEPLTAVVKFILETHHVVTRKAIDA
                     LPPLAARVRARHGELHPETRSVEGLVRRLVEDLGPHMLKEERILFPYIEALEAPGRGA
                     DACFPTVQNPIRVMLREHEAVEEILGELRAVTANYALPTDACESYHALYAGLEELEAD
                     LKRHIRIENDVLFPGAIALEAAG"
     gene            9304..11265
                     /locus_tag="PLB01_08935"
     CDS             9304..11265
                     /locus_tag="PLB01_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ77464.1"
                     /translation="MRDVAGTAADGALPVAGAPAPLAVEIPGEGYHRGLISCQAACPV
                     HTDARGYVRAIAEGRFEEAYLIARGPNPFASICGRVCGAPCEAACRRGKVPRVDDDGT
                     FVANDRPISIRALKRFVCSLAGPEARMPADVLEAVQGYVPSACSDADEMAALLRGAID
                     GRIPRTSGQKVAIIGAGPAGLSAAHDLALLGFSPVVYESEPVAAGMLAVGVPAYRLPR
                     ELIRREIAVIEALGVEIRCNVTVGKDVSFEELRRTHAAVLVAVGAKSSRALGLPGENG
                     PGVLGGVDLLRAVSLGEPLALGKDVVVVGGGNVAYDVARSVVRQIAWDAARTAARLPG
                     TSRVRLVSLEGLEEMPADTVEIREGDEEGVERLNGWGPVAIERDASGAVTGVTFRRCV
                     RVYDENRRFSPVYDDAIRQTLEADSVLLSVGQAPNLSFLAEGGRDVEEMRPGWPKVDP
                     ATLATTAPGVFVAGDLAHGTRLLIDAVASGKAAARSIYRALTGNALAPDTLTAFLTLP
                     GYRRERGYESIRRVTVPVAEPEERLKHPEAEVETGYTVEQAMREASRCLDCGVTPIFD
                     GSRCVLCGGCVDVCPTLCLKLVDLAGLAPSPELAAAIDRDLGPDPGEATAILKDEDRC
                     IRCALCVMRCPTDAIAMDRVTFATTWRTA"
     gene            11262..11747
                     /locus_tag="PLB01_08940"
     CDS             11262..11747
                     /locus_tag="PLB01_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQQ77465.1"
                     /translation="MTTTSVPTRSRLDPEPVARRDFLGLSALWAAGSALAFAMLGALR
                     LPKAAVLPSPSRKYKVTLPDTLAPGEAFVPPGRNVAVFRDAEGVFAVSTVCTHLGCIV
                     KPGPEGFDCPCHGSRFAKDGSVTKGPAPRALAWRKVSGSGGAYVVDEGEDVAPGTKVK
                     A"
     gene            11744..13306
                     /locus_tag="PLB01_08945"
     CDS             11744..13306
                     /locus_tag="PLB01_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQQ77466.1"
                     /translation="MSAAAPVPIRETALRRLVRNLKGAPRLVKDSAFRGGVPSTDRTR
                     STFVFGNVFLHLHPVRTHRWSLRWSTTWGLGIAAMAAFLVTLATGVLLMFYYKPHPLA
                     AYDSIKDIHFVVPTGRFIRNIHRWAANAMVVVVLLHMARVFFTAAYRQRREFNWLVGM
                     ALLATTLGLSFTGYLLPWDQLAYWAITIGANIAQSPREVTDALAITDRFDPGGFQKLL
                     LLGSDTVGEEALIRFYVLHVMLLPLVLAALAAVHFWRIRKDGGLARPADADARVGAPG
                     IDSLPVFTDSPRKTYQLAALVRGKSPAVGRGPEATVPSMPHLFYAELAVLMLTVAGCL
                     ALGILSDAPLKELANPSVPENPAKAPWYFLGLQELVSYSAFMGGIGIPMIVLLGLGLI
                     PFLDRETQGTGEWFGGPGGKKLVVQAAGVGLLSTLSIEALAIRFGWIREWFPHVPQLV
                     VTFLNPGTVLTAVFAAYSMWLVRRHDSTRAGALGLFTCFLCGFVVLTVIGSYFRGPNW
                     AFYWSHADWPAH"
     gene            13314..>13770
                     /locus_tag="PLB01_08950"
     CDS             13314..>13770
                     /locus_tag="PLB01_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77467.1"
                     /translation="MRNKHLLLWSSLLTLALLGAAAVHENFFREWRRVQAGASRPGAP
                     TEMRLRQVYVPALGAADRCVSCHVGMAPGEQVVAGHKVLGPHPKVAHEPADMGCTICH
                     GGQGRATDRADAHGEAPHWPEPMLPRRYAYAGCGGCHTPVGVPDAARLAA"
BASE COUNT         1829 a         5177 c         4646 g         2118 t
ORIGIN      
        1 cgcgcaggcc gcgtactgga gcggcgacta ccgttcggcg cgccgcgact tcgcgcgcgt
       61 tctcgccctg cgcccggaca gcccggtcgc ccggaagagc ctcgacgaga tcgcgagcgc
      121 ctcgcgcgcc cgctgggagg tctcggcagc cggcctctcg gacgaccagc cgtatcgcac
      181 ggcgggcctc gcggcgcgcg tctccgtctt ctccgacccc ctcacgcgct gggacttctc
      241 ggcgggcgga caggttctcc gcgccccgga cgcggcggac gaccgcaccg acgtgccgtg
      301 gggccgcgtg ggggtggaaa ccgtcttccc gtcgctgcgc ctcacggcgg ccgggtggct
      361 cgaggcgcgg cgcgcgccgg acgcgacgac gctgggcctc tggagcgtct ccctccggcg
      421 cgcgctcggc tccgcgggcg ccctgtcgtt cgtcgcggac cggcgcgaga tcttcgcgac
      481 gcgcgcgtcg ctcgcggacc acccgaacgt gacgcgcgcc ggcctcgcgt ggagctggga
      541 gtcgcgcgcg cacgcgagcg ccggggcgga ggcgttcctc ctccggtact tcgacggcaa
      601 ccgcggggtc gcggcatcgg cctgggcgct gtttccggtc ttcaccgccg gggcgttccg
      661 gctcaacgcg ggacccgcgg tctcgtaccg cgacacggac gagacgcgct tcgtcgtcgc
      721 cggcacggcg tcgacgccgg acgcggggtt cttccgctac gcgtacacgg gcgcgtacgt
      781 cccgtactgg acgccgctcg cgctcgcgga gggacgcgtc gcggccgtgc tcgagggccc
      841 gatcgcctcc ggcctgacgg cgcgcttcag cggagacgcc ggctgggcgc acgacgacgc
      901 cgtcggcttc ggtcccgaca gcggactctc ccccgctccg ggcgccacga cctccttcac
      961 gtaccgccgg accttccacc cgtggcgcgc ctcggtcacg ctcggcctgg acctcggcgg
     1021 cgggagccgg ctcgaggcag gctaccgcca cgaaaccacc gtctattaca ggagcgatgc
     1081 cctccatgca tccgtggtcg gacgcttctg agatcttccg ccaccggcgc gcgaagccgg
     1141 tggagacgtg ggagcgctgg gtcctcggcg tcctcctctt cctcgggctc cagagcgtcg
     1201 ccttcctggc gtggtggtgg ttccgtctcg agcacgtcca cgaccccgtc ctcttcgtgc
     1261 tcctctcgct cgcgacgtgg tacgggatct tccggatgct ggtcggctgg tacaacgcgt
     1321 ttcacgtcga gcagccgccg gagaagccgg cgcctccagg cctctcggtc gcgatcttca
     1381 cgacgtcgtc gcccggcgag ccttacgaga tgttcgtgcg tacgctcgcg gccgcccgcg
     1441 acgtgaggta cccccacacg acctatctcc tcgacgacac gggcgacccg cgcttcgcgg
     1501 ccctcgcgaa ggagatgggc gccgtgcccc tcgagctcgt cggcctcccc ggcgcgaagg
     1561 ccgggaagat caacgcggcg ctcgcgcgga cgacggagga gttcgtcctc gtcctcgacc
     1621 ccgaccacgt gccccttccg gagctcctcg accgggttct cggccacttc gacgacccgc
     1681 gcgtcggatt cgtccaggtc gcgcaggcgt accacaacgc cagcgagtcc ttcgtggcgc
     1741 ggggcgccgc cgagcagacg ttcgcgtact acggcccgat cctccagggc atgcacggca
     1801 cgggcacggc cgtggcggtg ggcgcgaact gcacgttccg gcggaaggcc ctcgaatcca
     1861 tcggcgggca cgggatcggc ctcgcggagg acctcgtgac gtccatccgg ctgcacgcgg
     1921 cgggctggac gtccgtctac gttcccgaag tcgtgacgcg cgggctcgtc cccgccgacc
     1981 tcgattccta tctcaagcag cagctcaagt ggtcgcgcgg cgtccacgag gtcctcttcc
     2041 acgagtaccc gaaggcgttc cggcggctga cggcgttcca gcggatctcg tacctcatga
     2101 tcgggacgta ctacctcgtc ggcctcacga cgccggtcta cgtcgcgatc ccgctcctct
     2161 atctccttct cgggctccag ccggccgcca tgctcctctc ggagtacctc ctgcacgcgc
     2221 tccccgtcgg tctctgcggc gtcgcgatct acctgttcat ccagcgctgg ctctgcgacc
     2281 ccgtccgcga gcggggcgtg catgggcgcg ggacgctcct gaaggtcggc tcgttcagcg
     2341 tctacctcaa ggggctcgtc ctcgccgtcc tcggcgttgc ggtgccctac atcccgacgg
     2401 cgaaggtccg gcagaccgga cgcttctggg ctctcgcgcg cctcccgctc ttcgtggtcg
     2461 ccctttcgct cgccgcgacc gcctggacga tcacccggag gctcttcgtc atccccgagg
     2521 ccgaagtcag gatctcgacg gaagtcacgc tcgggatgct aggctttctc gtgctcaacg
     2581 tcgtcttcat gagcggccgc atctacgccg cctggcaggg ccggccgaga ggaacttcgt
     2641 gacgccgcgg cgccggtacc gtgtggcgtt cttcgccctc ggctggctcc tcgcgctcgg
     2701 ggtcctgttc cagttcggat tcacgagcgt ctttacgcgc ctcgcggcga aggtctcggg
     2761 cgcgctggcc ggagtccagc aggccgtctc ggacgccgag ggccgcgcgc tcacgcgggc
     2821 gcgccacggg tcgcgccggg actatctcca gcccctcgcc gcgtcgctcg gttccgccga
     2881 cgcgcttcgg gacccgcgcc gcgttctctt tggcgcgtac gacggagcat tcccacagtc
     2941 cttcgaaggg ctcgagaagc tcgagcagca gctcgacttc cgcttcccgc tcgtctcgtt
     3001 ctacgtcgcg tggggcgaca agcccgatca gcagtttccg ctccgcttcg tcgagaccgt
     3061 ggaccgcatg ggctccgtcc cgatgatcac ctgggagccg tgggtcgtgg acttcgacgc
     3121 gaaacggcgg acgaaccttc tctcccgcgc ggagcgcgag tacgcctcgc tcgccgcgat
     3181 cgcgcgcggc gagtacgact tctacgtcgt cccgtgggcg gaggcggcgg cgcgctacgg
     3241 caagccgctg ttcctgaggt tcgcccacga gatgaacgac ccctatcgct acccgtgggg
     3301 cccgcaaaac gggaaccgcc cggcggactt catcgcggcg tggaagcacg tccaccaggt
     3361 cttccagaaa atgagcgcga cgaacgtcct ctgggtctgg tcgccgcaca tctcgatgcc
     3421 gtggttcgag tactactacc cggggcccga gtgggtggac tgggtcggct tcggcgtcct
     3481 caactacggg tccgtcgcga cgtggtcgcg gtggtggagc ttccatcaga tcgtcgagaa
     3541 gtcctacccc gcgctcctcg cgctcaagaa gccgctcatg atcggcgaat tcggtacgct
     3601 ttcctccgga ggcgacgccg tcgagtggta caagcaggct ttctaccacc tcgaccacac
     3661 gtaccagcgc gtccgcgccg tcgtattctt caaccagagc gcggacacga cgatgtcgac
     3721 ggtccccctc aactggagcc ccctccagga ccggcgcgtc tcttccctcg tcgcgcgcga
     3781 gatcgcgcgc aagtcggttc tcgagccatg acgaggttcg agcgcctccg ccccgcgctg
     3841 atcctgctcg ccggcgcgct cgccgcagcg cccgctgcgg gccagacggt cttcgtggac
     3901 ggcgccgtca cgatctctcc cgccgggtcg ccgctctccg ggatcacggt ggcggcgtac
     3961 acgctcggcg gcagcgtggc cgggacgacg acgagcgact ctggcggacg ctaccggctc
     4021 gccctcgtcg tcgggacgta ccggcttctc gcctacgacc tgagcggcgt ctgggcgacg
     4081 aacttctaca gcaacgcgac gtcgttcgac acgtccgccc aggtcaacct caccgcgagt
     4141 ctcgccggga tcgacttcca gatggcccgc ggaacctccg tctcggggcg cgtcgcgtcc
     4201 tcgcaggggg gatccctctc cgggatgacc gtggcggcgt acaacaccga cggcagccgc
     4261 cgcggcttcg agaagacgaa cgggagcggg gtcttctcca tcctcctgcc tcccggcacc
     4321 taccggttcg cggcgtacga cgacaacctc aactacgcga cgtcgttcta cctcggccgc
     4381 gccacgtacg acgccgcgac tccggtggcc gtttcctcca gcccggtcac gggcatcgac
     4441 ttcgtcctgt cccccgccgc ccaggtcttc gggagcacgt ccgaccgggc gacccggctg
     4501 gccatccccg gcatcaccgc gaacgcctac acgacgtccg gcgtcctcgt cggctcggcg
     4561 acgagcggcg cgaacggacg cttctcgatg gcgatcctcc cggggtccgt caagttcacc
     4621 gcgttcgatc cggccgggcg ctacgccgtc tcgttcttcg aggacgccgc cacgttcgcg
     4681 tccgcgcctt cgttcccgct cacggccggc cagagcatcg gcgcggtgaa cttcttcctc
     4741 gcgcccgcca caccgccctc cgcccccacg acgctcttcg ttcctgccgc cgtcaacgcg
     4801 ggcggcgcgg gcggggccta cttccagacg gacctgtgga tcacgaaccc ctccgacgct
     4861 gctctcgccg tcaccgtcac cttcctcccg gccggccagg acaactcggg ccggacgggg
     4921 acgccggtgg cggtcgcccc gcacgggcag gtcgcgttca cgaacgtgct ccagtccctc
     4981 ttcggggcgt ccggcgccgg ggccctgaag ctctcggcga gcgcctcgtt cctcgcgacg
     5041 agccggacgt acaacaacgc cgcgccgccc ggaacgtacg gcgtcggcgt cgccgggcgg
     5101 cccgcgtcgg cctccgtgag ccgcgggctc atccagggcc tcgcgaacac cgccgcgttc
     5161 cgctcgaacg tcgcgttgat gaacccgcag acgatccccg tgacggtcac gctcgcgctg
     5221 taccgctcgg acggcacgct cctcggccag gcgccgcgca cgctcgcccc gctggactgg
     5281 ttccaggcca gcacgatctt ctcgttcctc ggcgtctcca cgatcgagcc gaacgcctac
     5341 gtcctcgtcg gctcgccgga cggctccttt ttcgcgtacg gcgccgtcaa cgaccagacg
     5401 acgggcgacg gcaccgtcat cgaggccgtc ggctactgag cggcgcccgg cgcgctcacg
     5461 cgccggcgcg acgcacgaga agcggcttga gaaaccgcgc ctccacctcg gcggcgggga
     5521 gcgcccggct cacgaggtag ccctgcatct cgaggcagcc gtgctccctc aggaactcga
     5581 gctgtcccgc cgtctccacc ccctcggcca cggtgcggag cgcgatccgg ttcgcgagga
     5641 gaagggtcgc gatcacgatg gaatccacgt tcgcgttctg cgtgaggtcc cgcacgaagc
     5701 tctggtcgat cttcacgctg tcgatcggga accggcgcag gtagctcagc gacgactggc
     5761 cggtcccgaa gtcgtcgaga gcgatcccga cgcccatctc cttgagctcg tcgaggatcc
     5821 tgttcgacca gtccgacttc tggatcgcga ggctctccgt gatctcgagc tcgaggctgc
     5881 cgggcatgag gcccgtcttt ctcagcacgc ggcggaccat gcccatgaag tcccgctggt
     5941 agaactggcg ggccgagacg ttcaccgcga cgttcagctc cgtgaggccg gagtcctgcc
     6001 agaccttcat ctgccggcat gcctcgtgga gcacccactc gccgatcggg atgatcaggc
     6061 cggagtactc ggcgagggag atgaagccct tcggctcgac gaggacctgc tccgggtggt
     6121 tccagcggac gagcgcctcg aagcccacga tcgcgttcgt ccggatgttc acctgcggct
     6181 ggtaatggag gacgaactcg ccgcgctcga gtccgcggcg cagctcctcc tcgagcgaga
     6241 gccgcccgag cgccttctgg ctcggcacct gctcggcgag ctcgaagccg ttgccgccgg
     6301 ccaccttcac ccggtacatc gcgctgtcgg ccgaccggag gaggtcctcc ggggtctcgc
     6361 cgttccgcgg gtagatcgcc acgccgacgc tcgcggtgac gtgcagctcg tcgtcggcga
     6421 cgaagtaggg ctccgacacc ttcgcgagga tcttccgtgc gacgtccgcg gcctcttctt
     6481 cgccggggac gcccgggagg aggaccgtga actcgtcgcc gccgacgcgc gcaaccgtgt
     6541 ccccctcgcg gaggcacgcg cgcaggcggt ccgcgatcat gcggagcagc aggtcgcccg
     6601 tcgcgtgccc gagcgtatcg ttgacgacct tcaggtcgtc cacgtcgagg aacaggaccg
     6661 cgggcgtcgc ctgggtgcgg cgggcgagcg agagcgcgaa gcccatgcgg tccgcgaaca
     6721 ggttccggtt cgggaggagg gtgagctcgt cgtgcagcgc gcgccgctcg atctcctgcg
     6781 ccgccttctt gcggtccgtg atgtcgagga ggatgccctc gaggatcgcc tcctcgccgg
     6841 gcccgccgtc gatgagccgg acgctctcga gggcccagac aacctgtccg tcgcggcggc
     6901 ggagcgcgac ttcgtggttc gaaatggcgc cctcgcggcg caggcggacg agaagggcct
     6961 cgcggtccgc cgggtcctcc gtcgcgatcg tgccgccctg ctcgagaaac tggcgcgccg
     7021 agtcgaaccc gaagagtcgg gcgcaggcgt cgttgcagtc gagggcgcgc ccacccgcgg
     7081 tcacgacgta gacgcccgcg aggttgcgct cgacgaacag ccggtagcgc tcctcgaggc
     7141 cgcggttgcg cgacgcgagc tcgcgctgcg ccgcctcgag acggtcgacc atcccgttga
     7201 aggcgctcgc gaggcgcccg acctcgtcgt cgtggcgcac cggggcccgc gcggagaggt
     7261 cgccggccgc gatgacctgc gacgccgcca cgatgcgtcc gagaggctcc gtgaagacgg
     7321 tcgacagacc gaagaccgtg acgatgccgg ccaggaagat gacgaggcat acgagcgcga
     7381 tcgtccggcg cgtcccggcg atctcttcat tcaggcggtc gagcgagagg cccacggtca
     7441 cgcgtccaat gtcgcggccg ttgtaggaca cgggcgacgt cacctggtac acacggtcgt
     7501 ccggagagaa tcccccttcg ctgcgcggcc tcggcgcccc gggcgggatc gctccgaggc
     7561 gtctcggcgc catcgagatg gcgcgggacc cgatgcgttc ggcgaggtcc gcgttgaaac
     7621 cggccgcgag gcgtccgctc ctgtccgtga ccatcacgta gacgaggtcc gcgttccgcc
     7681 gcacggcgct gagagactcc tcgaggccgg gcccatcctc gaagtacagc gccgcgccga
     7741 ggtcgaacgc cgtcatctcc gtcatcgtga aggcgttctc cagcgtcgcc gcgaccgcct
     7801 gggtccgcag ccgcgagggc acctccacgt agatcgcgag cgagatgacg ccgaggaacg
     7861 cggagagcgc agtggcgagc ttcgtacgga gcgaccactg cgagaaacgc ttcacgggac
     7921 cggcctcgcg agcttcagga gcttcgagcc gaagtccgcg ccctcggcgc gcgacgcctc
     7981 ccggttgatc aggatccgcg gcgcgccccg gccggactcg aagccgacgg ccagcccgtc
     8041 ctccacgaac gccggaactc cggtcgccgt ccggatcttc aggctgcggc tgagcgtgac
     8101 gatccggccc acgtcgaagc cggcgagcgg cgcgacgtag agggcgcccg gatcgagcgc
     8161 ggcgacgcct tccgcgagcg caccgggagc cgagacgtcg atgaggccgg gccgcgcgcg
     8221 gagcgctccg tcgttcgcgg aggcccgttc gacctcggcg gcgaaccgct ccgcgacgtc
     8281 gcgagacgcg ggcgacgacc gctgatacac gattccgatc acgagcccgg ttcgttcccg
     8341 cgctccgagg ttccggtccg ccgcaaggac tttcaggagg agcgaggcct gcgtcgcgag
     8401 gggcacgggc tccgcgggcg ccgccgcgag agcggcgccg acggcccagg tcgcgaggga
     8461 aagacacccc atggaaccgg cccctccagc gtcgacgaat cgttcaggac ttctgcccgg
     8521 tatccgtccg cctagcttac gcccttcggc ggggcctttc cgccctccgg cctgatcggc
     8581 gtcatacggc ccggcgcgcg cgtctcctat cgtcgtgcac cgagggagga agacgatgca
     8641 gatcgagacc ggcacgccca cgagcacgga tcccgccggc cgcgcgtggg cgaaagagcc
     8701 cctgaccgcg gtcgtgaagt tcatcctcga gacgcaccac gtcgtcaccc gcaaggcgat
     8761 cgacgccctg cctcccctcg ccgccagagt gcgcgcccgg cacggcgagc tgcaccccga
     8821 gacgcggagc gtggaaggcc tcgtacggcg gctcgtcgag gacctcggcc cgcacatgct
     8881 caaggaggag cggatcctct tcccgtacat cgaggcgctc gaggcgccgg ggcgtggagc
     8941 ggacgcgtgc ttccccaccg ttcagaaccc gatccgcgtg atgctgcgcg agcacgaggc
     9001 cgtcgaggag atcctcggcg agctgcgcgc cgtcacggcg aactacgccc tccccacgga
     9061 cgcctgcgag tcctatcacg cactctatgc cggcctcgag gaactcgagg ccgacctgaa
     9121 acggcacatc cggatcgaga acgacgtcct gttccccggg gcgattgcgc tggaggcggc
     9181 gggctgaacg ggcggcggcg ggcttcgccg gccggaccta cattcgtccg gcgcgtatga
     9241 cgctcgtcat accaggcccc tccggagcag cctagcgtcc ccggagagac cgcgggagcc
     9301 agaatgcgtg acgtcgccgg gaccgccgcc gatggcgctt tgccggttgc cggtgcgccc
     9361 gccccgctcg ccgtcgagat ccccggggag ggctaccacc gggggctcat ctcctgccag
     9421 gccgcctgtc ccgtgcacac cgacgcgcgc ggctacgtgc gcgcaatcgc cgaagggcgc
     9481 ttcgaggagg cctacctcat cgcccgcggg ccgaacccgt tcgcgtcgat ctgcggtcgc
     9541 gtctgcggcg cgccgtgcga ggccgcctgc cggcgcggga aggtgccgcg cgtggacgac
     9601 gacgggacgt tcgttgcgaa cgaccgcccg atctcgatcc gcgcgctcaa gcgcttcgtc
     9661 tgttccctcg cggggcccga ggcccggatg ccggcggatg tcctggaggc ggtccagggc
     9721 tacgtgccga gcgcctgctc ggacgccgac gagatggcgg cgcttctcag gggcgcgatc
     9781 gacgggcgca tcccgcgcac ctccggccag aaggtcgcga tcatcggcgc gggcccggcc
     9841 ggcctctcgg ccgcccacga cctcgcgctc ctgggctttt cgcccgtcgt ctacgagagc
     9901 gagcccgtcg ccgccggcat gctcgcggtc ggcgtgccgg cctaccggct tccgcgcgag
     9961 ctcatccggc gcgagatcgc ggtgatcgag gcgctcggcg tcgagatccg ctgcaacgtc
    10021 acggtgggaa aagacgtctc gttcgaggag ctgcgccgga cgcacgccgc cgtcctcgtc
    10081 gctgtcggcg cgaagagctc gcgcgcgctc ggcctgcccg gcgagaacgg acccggcgtc
    10141 ctcggcggcg tggacctcct gcgcgcggtc tccctcggcg agcctctcgc gctcggaaag
    10201 gacgtcgtcg tggtgggagg cggcaacgtc gcgtacgacg tcgcgcgctc ggtcgtgagg
    10261 cagatcgcat gggacgccgc gcgcacggcc gcgcgcctcc ccggcacgag ccgcgtccgt
    10321 ctcgtctcgc tcgaagggct cgaggagatg cccgccgaca ccgtcgagat ccgcgagggc
    10381 gacgaggaag gcgtcgaacg cctgaacggc tgggggcccg tcgcgatcga gcgcgacgct
    10441 tccggcgcgg tcacgggcgt cacgttccgg cgctgcgtgc gcgtctacga cgagaaccgg
    10501 cggttctcgc ccgtctacga cgacgcgatc cgccagaccc tcgaagccga cagcgttctc
    10561 ctctccgtcg gccaggcgcc gaacctctcc ttcctcgccg aaggcggcag ggacgtcgag
    10621 gagatgcgcc ccggctggcc gaaagtcgac cccgcgacgc tcgcgacgac ggcgcccggc
    10681 gtcttcgtcg cgggcgacct cgcgcacggc acgcgcctcc tgatcgacgc ggtcgcctcg
    10741 ggcaaggcgg cggcccgctc catttaccgc gcgctcacgg ggaacgcgct ggcccccgac
    10801 acgctcacgg ccttcctgac gctccccggc taccggcgcg agcgcggcta cgagtcgatc
    10861 cgccgcgtca ccgtgccggt cgccgagccc gaagaacgcc tgaagcaccc ggaggccgaa
    10921 gtcgaaaccg ggtacacggt cgaacaggcg atgcgcgagg cttcccgctg tctcgactgc
    10981 ggcgtcaccc cgatcttcga cggctcgcgc tgcgtcctct gcggcggctg cgtggacgtc
    11041 tgcccgacgc tctgcctcaa gctcgtcgac ctcgcgggcc tcgcgccgtc gccggagctc
    11101 gcggccgcga tcgaccgcga cctcggcccg gaccccggcg aggccacggc gatcctcaag
    11161 gacgaggacc gctgcatccg ctgcgcgctc tgcgtgatgc gctgcccgac cgacgcgatc
    11221 gcgatggacc gcgtcacctt cgccacgacg tggaggaccg catgacgacg acctccgttc
    11281 ccacgcgctc gcggctcgac cccgagcccg tcgcgcggcg cgacttcctc ggcctctccg
    11341 cgctctgggc cgccggctcg gcgctcgcgt tcgcgatgct cggcgcgctc cgcctcccga
    11401 aggccgccgt cctgccgtct ccctcgcgca agtacaaggt cacgctcccc gacacgctcg
    11461 cgcccggcga agcgttcgtc ccgccgggcc gcaacgtggc cgtcttccgc gacgcggaag
    11521 gcgtcttcgc agtgtccacg gtctgcacgc acctcggctg catcgtgaag cccggccccg
    11581 agggcttcga ctgtccctgc cacggctcgc gcttcgcgaa ggacggctcc gtcacgaagg
    11641 ggcccgcgcc gcgcgctctc gcctggcgca aggtttcggg aagcggcggc gcgtacgtcg
    11701 tggacgaggg cgaggacgtc gcgcccggaa cgaaggtgaa ggcatgagcg ccgccgcccc
    11761 cgttcccatc cgcgagacgg ccctccgccg cctcgtccgc aacctcaagg gcgcgccgcg
    11821 cctcgtaaag gattccgcgt tccggggcgg cgtgccctcg accgaccgga cgcgctcgac
    11881 gttcgtcttc gggaacgtct tcctccacct ccaccccgtg cgcacgcacc gctggagcct
    11941 ccgctggtcc acgacgtggg gactcgggat cgccgcgatg gcggcgttcc tcgtgacgct
    12001 cgcgacgggc gtcctcctca tgttttatta caagccccac cccctcgcgg cgtacgactc
    12061 gatcaaggac atccacttcg tcgtcccgac gggccggttc atccggaaca ttcaccgctg
    12121 ggccgcgaac gcgatggtcg tcgtcgtcct cctgcacatg gcgcgcgtct tcttcacggc
    12181 ggcgtaccgg cagcgccgcg agttcaactg gctcgtcggc atggcgctcc tcgcgacgac
    12241 gctcggcctc tcgttcacgg gctacctcct cccgtgggac cagctcgcgt actgggcgat
    12301 cacgatcggc gccaacatcg cgcagtcccc gcgcgaggtc acggacgcgc tcgcgatcac
    12361 ggaccgcttc gacccgggcg gcttccagaa actcctcctc ctcggctccg acaccgtcgg
    12421 cgaggaggcg ctcatccggt tctacgtcct ccacgtcatg ctcctgccgc tcgtcctcgc
    12481 cgcgctcgcg gccgtccact tctggcggat ccggaaggac ggcggcctcg cgcggccggc
    12541 cgacgcggat gcgcgcgtgg gggcgccggg gatcgactcg ctgcccgtgt tcaccgattc
    12601 cccccgcaag acgtatcagc tcgcggcgct cgtgcgcggc aagtcgcccg cggtcgggcg
    12661 cgggcccgag gcgacggttc cctccatgcc gcacctcttc tacgccgagc tcgcggtcct
    12721 catgctcacc gtcgcgggct gcctcgcgct cgggatcctg tccgatgccc cgctcaagga
    12781 gctcgcgaat ccctccgtgc ccgagaaccc ggcgaaggcg ccgtggtact tcctcgggct
    12841 gcaggagctc gtctcgtact cggcgttcat gggcggcatc gggatcccga tgatcgtcct
    12901 cctcgggctg ggcctgatcc cgttcctcga tcgcgagacg caggggaccg gcgagtggtt
    12961 cggcgggccg ggcggaaaaa agctcgtcgt ccaggcagcg ggcgtcggcc ttctctcgac
    13021 gctctcgatc gaggcgctcg cgatccgatt cgggtggatc cgcgagtggt tcccgcacgt
    13081 gccgcagctc gtcgtgacgt tcctgaaccc cggcaccgtc ctcacggccg tcttcgcggc
    13141 gtactcgatg tggctcgtga ggcgccacga ctccacgcgc gccggtgcgc tcggtctctt
    13201 cacgtgcttc ctctgcggct tcgtcgttct gaccgtcatc gggtcgtatt tccgcggtcc
    13261 caactgggcc ttctactggt cccacgcgga ctggccggcg cactgaggcg gccatgagaa
    13321 acaagcacct cctgctctgg agcagcctcc tgacgctcgc gcttctcggg gccgcggcgg
    13381 tgcacgagaa cttcttccgg gagtggcgac gcgtccaggc cggcgcctcg cggcccggcg
    13441 cccccaccga gatgaggctg cggcaggtct acgtccccgc gctcggcgcg gccgaccgct
    13501 gcgtgagctg ccacgtcggg atggcgccgg gcgaacaggt ggtcgcgggc cacaaggtgc
    13561 tcgggcccca cccgaaggtg gcgcacgagc cggcggacat gggctgcacg atctgtcacg
    13621 gcggccaggg tcgcgcgacg gatcgcgccg acgcccacgg cgaggctccg cactggcccg
    13681 agccgatgct cccgcgccgc tacgcgtatg ccgggtgcgg cggctgccac acgcccgtcg
    13741 gcgtccccga cgccgcgcgc ctcgccgccg
//
LOCUS       DAOMFY010000086        13628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1388_length_13628_cov_7.945848, whole genome shotgun sequence.
ACCESSION   DAOMFY010000086 DAOMFY010000000
VERSION     DAOMFY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13628
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1388_length_13628_cov_7.945848"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PLB01_08955"
     CDS             complement(<1..1162)
                     /locus_tag="PLB01_08955"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HQQ77468.1"
                     /translation="MATTAAPEAKAQEGSAKMRDSLTITDNRTGKAYDLPIKDGTIRA
                     IDLRQIKEQPTDFGLMTYDPAFLNTASCKSTITFIDGDKGILNYRGYPIAELAEKTTY
                     LETAYLVVNGELPTKAQYESWTNDITFHTMVHENIKTLMKGFRYDAHPMGMLASTVAA
                     LSTFYPEAKEVREPETRRQQIIRLIAKIPTLAAFAYRHSIGWPFSYPDNELSYCANFL
                     QMMYRAVEPKYVANPTLVKALEILFILHADHEQNCSTNAMRAIGSSEADPYVSAAGAI
                     GALYGPLHGGANEAVLRMLFEIGSKSKIPEFIKSVKESGGETKLMGFGHRVYKNYDPR
                     AKVIKNMAEQVFEVTGKNPLLDIALELERIALEDEYFVKRKLYPNVDFYSGLI"
     gene            complement(1317..1787)
                     /locus_tag="PLB01_08960"
     CDS             complement(1317..1787)
                     /locus_tag="PLB01_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ77469.1"
                     /translation="MAVPEDALRRHLVRALEWGEAHLALEKAVKGLPAALRGKRPRGL
                     AHSPWELLEHTRLAQRDLLDFCRDPDYEEGVFPDSYWPATPAPPSARAWKASLEAFAA
                     DRRAFQQFVETTPDLLAEIPGYPGKTILRSVLLAVDHTGYHVGQLVLTRKLLGA"
     gene            complement(1801..2238)
                     /locus_tag="PLB01_08965"
     CDS             complement(1801..2238)
                     /locus_tag="PLB01_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQQ77470.1"
                     /translation="MANLLRKSSRPAITVQETSTVLEAVKTMVRERIGAVVVMKGKKM
                     TGIFSERDVLDKIVLARRDPAKTPISAVMTPDVLSIPAPGDEAVAAEKMAANHIRHLP
                     IVDEKKKVVGVVSLRHVMEERIADLEHEVNVLEAYLGYDGVSG"
     gene            complement(2329..3117)
                     /locus_tag="PLB01_08970"
     CDS             complement(2329..3117)
                     /locus_tag="PLB01_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQQ77471.1"
                     /translation="MRVALLQMDLAWEDWKSNHAGAAKLLRRAADEGAALALLPEMFA
                     TGFSMDGGKIAQPPGGPSEQWLRGMARGLGLHLVAGLAETCDASGAPSPLPCNNALWV
                     TPEGDVSRYTKLHPFSFAGEDAVYAKGSKVVTWTIEGLRVTPLVCYDLRFPEPFRLAA
                     DGTDLFAVVANWPERRRHHWRLLLQARAVENLCYVAGVNRVGEDGKGNRHMGDSSVTS
                     PWGETLVSAAEAETVLIADVDPAEVADARAKFPALDDRRPAYSR"
     gene            complement(3129..4457)
                     /locus_tag="PLB01_08975"
     CDS             complement(3129..4457)
                     /locus_tag="PLB01_08975"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQQ77472.1"
                     /translation="MRLAAAALALLAFAPPAEPSVPPEALASAIESVVAPLAERDVFG
                     GVVLVARGDEVLFEKAWGMANQEFSVPNRPDTRFRIASITKRFTMLLFQQLAAEKKLS
                     LDDPLAKWAPAFPKADRITIAHLLNHKSGIRDPESLRRTIPASLTAEEVVALLAKTPL
                     ASEPGETYSYTTANYAVLAYVLERVEGSPFAAVVRRRLYAPAGMTDAGDLDTISVVPR
                     LASGYMPDPLAARGLAVSGPEDTSWKIGGGSGYATARDLLRFHRALAAGKLLPAGADP
                     LSVFPPTKARGRRVISSSGAFPGANARVLYFPDEGVTVVVLSNNYANVTGPIADSAAA
                     AAFGESVRPIEPPARGEAAPLDPRIAGSYLVESFPMPVTLEASHGKAVAVWSPARVSA
                     VLRIGPDEWFMPLDWATLKLVPGEGGAPELVVTAPWIGKPIKVTRVPQKP"
     gene            complement(4518..7367)
                     /locus_tag="PLB01_08980"
     CDS             complement(4518..7367)
                     /locus_tag="PLB01_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HQQ77473.1"
                     /translation="MDATDPALAAALAAAIGQDRVLTRAIDRIAWASDASFYRLVPQA
                     VVHARTADDVRRLFAVSRAHGVPLVFRAAGTSLSGQSVTDGVLVETARGFRGLTVEDG
                     GARIRVQPGVIGAHANRALAPFGRKIGPDPASIATCTLGGIVANNASGMCCGVTQNAY
                     HTLESLTFVLPSGTAIDTARPEADDVLRSKEPALWQGLADLRARVLSDAALAARIRAK
                     YRTKNTTGYALNAFLDFEHPVDVFARLLVGSEGTLAFVAEAVLATVPDLPVKVTGLLL
                     FPDLHAACSAIVPLNAAGAKALEVMDRAALRSVENEPGVPPALRALPPGAAGLLAEFQ
                     AAREDEREEIGRAAADAAAGLSLAEPARFTHDAAEQARLWHVRKGMFPSVGAVRKSGT
                     TVIIEDVAFPVDRLADAATDLTGLFARHGYADAIIFGHARDGNLHFVLSQSFRERRAV
                     DQYARFMDDVVRLVVGRYDGALKAEHGTGRNMAPFVETEWGPEAYAVMKRVKELADPA
                     GLLNPGVVVNADPRAHLKDLKPLPSVEEEVDRCIECGFCEPLCPSRDLTLTPRQRIVV
                     RRELARLDAARRDPRLRSALEADFPYDALDTCAVDGLCATACPVKIDTGTLTKRFRAV
                     RHSAAANRTAALVARSFRLVEPALRTGLRAGHAVQALFGARAMTGLTRALRAVAGDSF
                     PLWSAEMPQAAGKSRARTSAAAAEAIYFPSCLSRTMGPEAGDDFPSVVDTLVALAERA
                     GVAVHVPPDAAGTCCGVPFSSKGYAEAHAIAANRAVANLWRWSDGGRLPVFVDTSPCT
                     YGLLHAREALTDENRKRFDALTILDGVAFVHDVLLPKLAVTKKDASAVLHPVCSLVKM
                     GLVPKLEAVARACAETAVVPEGAGCCGFAGDRGFLVPELTASATRLESAEARGARAAG
                     HWSSSRTCEAGLSRATGERYRSFVHLVERATRPSP"
     gene            7410..8822
                     /gene="leuC"
                     /locus_tag="PLB01_08985"
     CDS             7410..8822
                     /gene="leuC"
                     /locus_tag="PLB01_08985"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQQ77474.1"
                     /translation="MGRSLLSKVWDLHAVRKLPTGQTQLFVGLHLVHEVTSPQAFAML
                     KENGWKVAFPSRTVATVDHIIPTDSLARPFADAQAEEMLVSLQENCRTFGVPLHDLGA
                     PGQGIVHVIGPELGLTQPGMTIACGDSHTSTHGAFGALAFGIGTSQVRDVLASQCLAM
                     DPMRLRRIDVAGRLAPGVYAKDVILAIIRTLGVKAGAGYAYEYGGAAVDAMSMDERMT
                     LCNMSIEGGARAGYVNPDATTFAYLKGRPAAPEGGAWDRAVAWWSSMASDADAAYDDR
                     VTLDGGALEPHVTWGINPSQSTGISGRVPKAADAPLEGRAQVAEALEYMGFAGGEPIA
                     GTKIDVAFIGSCTNGRLSDLREAARVVKGRKVAKGVKALAVPGSNAVRLAAEREGLDA
                     VFRDAGFEWRGAGCSMCLAMNPDRLEGRQVCASSSNRNFKGRQGSPTGRTLLMSPAMV
                     AAAAVAGEVADVRTLIGGAR"
     gene            8825..9424
                     /locus_tag="PLB01_08990"
     CDS             8825..9424
                     /locus_tag="PLB01_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQQ77475.1"
                     /translation="MPAIRRVSGTARPLLGDDIDTDRIIPARFLVSVTFDGLGAHAFE
                     DDRKAARARGEVHPFDDARFSGASLLLVNRNFGCGSSREHAPQALLRGGIKAVAGESY
                     SEIFFGNSVAIGLPCVTLPHDAMAALQELVARDPALSLALDLETKTLAAGEATFPVQM
                     PENARQAFLSGHWDGTSMLLDRFEAVEELAKRLPYVAGF"
     gene            complement(9435..9905)
                     /locus_tag="PLB01_08995"
     CDS             complement(9435..9905)
                     /locus_tag="PLB01_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013693037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HQQ77476.1"
                     /translation="MSFRIEGLAPDPFQPLFDLDDRALSARGVRRRVVDAKPGYPCRV
                     SLQDAELGEDVLLLPWEHHATPSPYRASGPIYVRKSAVKAWAGAPGSVPPMLRHRLLS
                     VRAYDAEGLMVGAEVREGSDLEEAISRLFANPSAAYLHVHNAKPGCFVCRVDRA"
     gene            complement(10024..11103)
                     /locus_tag="PLB01_09000"
     CDS             complement(10024..11103)
                     /locus_tag="PLB01_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77477.1"
                     /translation="MLRKLAALAVFALAALAFPTDAAKPDNNMPVSIMTQNMDDGTDL
                     TYVVAALLPIPLLPLEAAVDLTYYELIASAIPYRVTAMATEIAYRKPHLVAVQEAALW
                     RTGPAPDKATEPLSDSLDTLVSALQDLGAPYKVVAVNDLFDIAVPGATLGALRFTDRN
                     ALLVRADLKPPQFHLSNVHANTFSAAFLFAGLTVPAGWITADVHVGNKQFRVVTTHLQ
                     SPILGVSEATDVQVAQAQELLHALRNLQVPVVICGDFNSDANHGGFIDSTPTVDLIEA
                     AGYEDVWVKVYGGASGLTWPYYLEDQFPPPPFFADSSPYERIDLFFSKGMNPIGAELV
                     ILPFGSLPVPPHASDHAGVIATFRP"
     gene            complement(11256..12143)
                     /locus_tag="PLB01_09005"
     CDS             complement(11256..12143)
                     /locus_tag="PLB01_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQQ77478.1"
                     /translation="MEVAALRVFLTVAEERGFSKAAVRLFRTQSAVSQAVRRLEDELK
                     ADLFDRTTHDVRLTEAGKTLLGYAERVLAVADEAERAVRDLKDVKRGRVVIGANEAGL
                     EVVIPLIGRFRETHPGVEFEVKRSPSRLLGAEVLAVALDFGVVAFVPPEEGLKTVVLA
                     SDELVVLSHPSHPFAKKHRVTLEEFGRETVIAHSDPSPARERVLRTYSERGVALNIKV
                     SLPSLDGVKRAVERGMGVALLPRRTALSEIEAGRLAATTLPELRRPRDLRLVYRAAGN
                     FGPAAHAFLEMVRKERAAG"
     gene            12377..>13628
                     /locus_tag="PLB01_09010"
     CDS             12377..>13628
                     /locus_tag="PLB01_09010"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQQ77479.1"
                     /translation="MEARGNVKSDAIKKGPARAPARAMLRATGLTPADLEKPLVAVVH
                     PWTDLGPCNFSLRALAEEVKAGIREAGGTPFEFGTIAISDGISMGTDGMRASLVSREV
                     IADSIELAVSGHALDAVVALCGCDKTIPGAAMALARLDIPSVALYGGSIEPGKVAGRD
                     VTIQDVFEAVGAHAAGTISDQKLCDVEAGACPGAGACGGQFTANTMATALTFLGLSPM
                     GANDVPATDPAKAGVARAVGRLVMDRLKDGLTPSKILTRQAFENAIASVAATGGSTNA
                     VLHLLAIAREAGVPLVLDDFDSISARTPLFVDLKPGGRFTAPDLARAGGSRLVARRLV
                     EAGLAKNARTVSGRTLYEEAQDAVETPGQDVVRPVSKALKPHGGLAILRGTLAPEGAV
                     VKLAGHERMRHTGPARVFDGEAAAF"
BASE COUNT         1958 a         4571 c         5090 g         2009 t
ORIGIN      
        1 agatgaggcc cgagtagaag tcgacgttcg ggtagagctt ccgcttgacg aagtactcgt
       61 cctcgagggc gatccgctcg agctcgagcg cgatgtcgag gagcgggttc ttgcccgtga
      121 cctcgaagac ctgctcggcc atgttcttga tgaccttggc gcgcgggtcg tagttcttgt
      181 agacgcggtg gccgaagccc atgagcttcg tctcgccgcc ggactccttg accgacttga
      241 tgaactccgg gatcttcgac ttcgagccga tctcgaacag catccggagg acggcctcgt
      301 tggcgccgcc gtggagcggg ccgtagaggg cgccgatcgc gccggccgcc gagacgtacg
      361 ggtcggcctc ggaggagccg atcgcccgca tcgcgttcgt cgagcagttc tgctcgtggt
      421 ccgcgtggag gatgaagagg atctcgagcg ccttcacgag cgtcgggttc gcgacgtact
      481 tcggctcgac cgcgcggtac atcatctgga ggaagttcgc gcagtaggag agctcgttgt
      541 ccgggtacga gaacggccag ccgatcgagt ggcggtacgc gaacgcggcg agcgtcggga
      601 tcttcgcgat gaggcggatg atctgctgcc ggcgggtctc gggctcgcgg acttccttgg
      661 cctcggggta gaacgtcgag agcgcggcga ccgtcgacgc gagcatcccc atcgggtgcg
      721 cgtcgtagcg gaagcccttc atgagggtct tgatgttctc gtggaccatc gtgtggaacg
      781 tgatgtcgtt cgtccacgac tcgtactgtg ccttcgtcgg gagctcgccg ttcacgacga
      841 ggtacgccgt ctcgaggtac gtcgtcttct ccgcgagctc ggcgatcggg tagccgcggt
      901 agttcaggat gcccttgtcc ccgtcgatga acgtgatcgt gctcttgcac gacgccgtgt
      961 tcaggaacgc cgggtcgtag gtcatgaggc cgaagtcggt gggctgttcc ttgatctggc
     1021 gcaggtcgat cgcgcggatc gtcccgtcct tgatcggaag gtcgtacgcc tttccggtcc
     1081 ggttgtcggt gatcgtcagg gaatcacgca tcttcgcgga accctcctgc gccttcgctt
     1141 cgggcgcggc cgtcgtcgcc atattcattt cctcctctgg gcccggctgg gcctccgggt
     1201 cgtggcagaa gaatggggac gcgcgaacag cccggaaatg atgaggatgc gctagttctc
     1261 cgacgtttgt gacgaaattc acaagcgcgg gcccgccgcg ggccgggggc cgggtctcag
     1321 gcgcccagga gctttcgcgt caggacgagc tggcccacgt ggtaacccgt gtggtcgacc
     1381 gcgaggagca ccgagcgcag gatggttttt ccggggtagc cggggatctc ggcgagcaga
     1441 tcgggggtcg tctcgacgaa ctgctggaac gccctgcggt cggcggcgaa ggcctcgagg
     1501 ctggctttcc aggcccgggc ggagggcgga gcgggggtgg cgggccagta cgagtcgggg
     1561 aagacgcctt cctcgtagtc cgggtcgcgg cagaaatcga ggagatcccg ctgggcgagg
     1621 cgggtgtgct cgaggagctc ccacggagag tgggcgaggc ccctcggccg tttcccacgc
     1681 agtgcggcgg gcaggccttt cacggccttt tctagggcga gatgggcctc gccccactcg
     1741 agcgcgcgga cgaggtgacg gcggagggcg tcttccggga cggccatgcc gtgaacggta
     1801 tcagccggag acgccgtcgt agccgaggta ggcctcgagg acgttcacct cgtgctcgag
     1861 gtccgcgatc cgctcctcca tgacgtgccg cagggagacg actccgacga ccttcttctt
     1921 ctcgtcgacg atcgggaggt ggcggatgtg gttcgccgcc atcttttcgg cggcgacggc
     1981 ctcgtcgccg ggggccggga tcgagaggac gtccggcgtc atgacggccg agatcggggt
     2041 cttggccggg tcgcgccgcg cgaggacgat cttgtcgagg acgtcgcgct cggaaaagat
     2101 gcccgtcatc ttcttgccct tcatgacgac gacggcgccg atgcgctccc gaaccatcgt
     2161 cttgacggcc tcgaggaccg tggaggtctc ttgaacggtg atcgccgggc gggaactctt
     2221 ccgaagcagg ttcgccatgg cggactcctt tcgcaggcgg gcgcctggga acaatctggg
     2281 agccgccggc ggagccggcc ttgatgcaag tggactaggg aacgggcgtc atcgcgagta
     2341 ggcggggcgc cggtcgtcga gcgccgggaa cttcgcccgc gcgtcggcga cctcagccgg
     2401 gtcgacgtcg gcgatcagga ccgtctcggc ctcggccgcg ctgacgagcg tctcgcccca
     2461 cggcgacgtg acggacgagt cccccatgtg ccggttgccc ttgccgtcct cgcccacgcg
     2521 gttcacgccc gccacgtaac agaggttctc gacggcccgc gcctgcagga gaagccgcca
     2581 gtggtgccgc cggcgctcgg gccagttcgc gacgacggcg aagaggtccg tcccgtccgc
     2641 cgcgaggcgg aagggctccg ggaagcgcag gtcgtagcag acgagcggcg tgacgcgcag
     2701 cccctcgatc gtccaggtga cgaccttcga tcccttcgcg taaaccgcgt cctcacccgc
     2761 gaaggagaac gggtggagct tcgtgtaccg cgagacgtcg ccttcgggcg tcacccagag
     2821 cgcgttgttg cacggcagcg gcgacggcgc gcccgaggcg tcgcacgtct cggcgagccc
     2881 ggcgaccagg tgcagcccga ggccgcgcgc catcccgcgc agccactgct cgctcggccc
     2941 gcccgggggc tgggcgatct tcccgccgtc catcgaaaag cccgtcgcga acatctcggg
     3001 cagcagcgcg agcgccgcgc cctcgtcggc cgcgcgcctg agcagcttcg ccgcgccggc
     3061 gtggttcgac ttccagtctt cccaggcgag gtccatctgg aggagggcga cgcgcatccg
     3121 gggggagatt acggcttctg cggcacgcgc gtcaccttga tcggctttcc gatccacggg
     3181 gccgtcacga cgagctccgg cgcgccccct tcgcccggga cgagcttcag ggtcgcccag
     3241 tcgagcggca tgaaccattc gtcgggcccg atgcgcagga ccgcgctcac gcgcgcgggg
     3301 ctccagacgg cgacggcctt cccgtgcgac gcttcgaggg tcacgggcat cgggaagctt
     3361 tccacgaggt acgacccggc gatccgggga tcgagcggcg ccgcctcgcc gcgtgcgggc
     3421 ggttcgatgg gcctgacgct ctccccgaat gcggcggcgg cggcggaatc ggcgatgggt
     3481 cccgtgacgt tcgcgtagtt gttcgacagg acgacgaccg tcacgccctc gtcggggaag
     3541 tagaggacgc gcgcgttcgc gcccgggaac gcgccgctcg aggagatcac acggcggccg
     3601 cgcgccttgg tcggcggaaa gacggagagc gggtcggccc cggcgggcag gagcttgcct
     3661 gcggcgagcg cgcggtggaa gcgcaggaga tcgcgcgccg tcgcgtaccc cgagccgccc
     3721 ccgatcttcc aggacgtgtc ctcggggccg ctcacggcga gcccgcgcgc cgcgagcggg
     3781 tcgggcatgt agccggaggc gaggcgcggg accacggaga tcgtgtcgag gtctccggcg
     3841 tccgtcatgc ccgcgggcgc gtagaggcgc cgcctgacga cggccgcgaa cggggagccc
     3901 tccacgcgct cgaggacgta cgcgaggacc gcgtagttgg ccgtcgtgta ggagtacgtc
     3961 tcgccgggtt cgctcgcgag cggagtcttc gcgaggagcg cgacgacctc ctcggccgtg
     4021 aggctcgccg ggatcgtccg ccgcaggctc tccgggtcgc ggattccgga cttgtggttc
     4081 aggaggtgcg cgatcgtgat ccggtccgcc ttcgggaacg cgggggccca cttcgcgagc
     4141 gggtcgtcga gcgagagctt cttctcggcg gcgagctgct ggaagaggag catcgtgaac
     4201 cgcttcgtga tggacgcaat ccggaaccgg gtgtccgggc ggttcgggac ggagaactcc
     4261 tggttcgcca ttccccacgc cttctcgaag aggacctcgt ccccccgcgc gacgaggacg
     4321 acgccgccga agacgtcgcg ctccgcgagc ggcgcgacga ccgactcgat ggcgctcgcg
     4381 agcgcctccg gagggacgga aggctccgcg ggaggggcga acgcgaggag cgcgagggcg
     4441 gcggcggcga ggcgcatgaa cgtcctcctt tcaggtgccc gagagacgcc gggccgcgcg
     4501 gaaagttcct ccgcacgtca gggagaggga cgcgtcgcgc gctccacgag gtggacgaac
     4561 gagcggtagc gttcgcccgt cgcgcgcgag aggccggcct cgcacgtgcg gctggacgac
     4621 cagtgccccg ccgcgcgggc gcctcttgct tcggcggatt cgagccgcgt ggccgaggcg
     4681 gtcagctccg ggacgaggaa gccgcggtcg cccgcgaagc cgcagcagcc cgcgccctcc
     4741 gggaccacgg ccgtctccgc gcacgcgcgg gccacggcct cgagcttcgg gacgaggccc
     4801 atcttcacga gcgagcagac gggatgcagg acggccgagg cgtccttctt cgtgacggcg
     4861 agcttcggga ggagcacgtc gtggacgaac gcgacgccgt cgaggatcgt gagagcgtcg
     4921 aagcgcttcc ggttctcgtc cgtgagggct tcgcgcgcat ggagcagccc gtacgtgcat
     4981 ggagatgtgt ccacgaagac gggcagccgg ccgccgtccg accagcgcca gagattcgcg
     5041 accgcgcggt tcgccgcgat cgcgtgcgcc tcggcgtagc ccttcgacga gaacgggacg
     5101 ccgcagcacg tgccggccgc gtccggggga acgtggacgg cgacgccggc gcgctccgcg
     5161 agcgcgacga gcgtgtcgac gacagacggg aagtcgtcgc ccgcctccgg ccccatcgtc
     5221 cgcgagaggc aggacgggaa gtagatcgcc tctgccgccg ccgcggaagt ccgcgcgcgg
     5281 gacttgcccg cggcttgcgg catctccgcg ctccagagcg ggaaggagtc gcccgccacc
     5341 gccctcagcg ccctcgtgag gcccgtcatc gcccgtgccc cgaagagcgc ctgcacggcg
     5401 tgcccggccc tgaggcccgt gcggagcgcg ggctcgacga ggcggaacga gcgcgccacg
     5461 agcgcggcgg tgcggttcgc cgccgccgag tggcgaacgg cgcggaagcg cttcgtgaga
     5521 gtgcccgtgt cgatcttcac cgggcacgcg gtcgcgcaga gtccgtccac ggcgcacgtg
     5581 tcgagcgcgt cgtacgggaa gtcggcctcg agggcggaac ggagccgagg atctctccgc
     5641 gccgcgtcga gacgggcgag ctcgcggcgc acgacgatgc gctggcgcgg cgtgagcgtg
     5701 aggtcgcggc tcgggcagag cggctcgcag aagccgcact cgatgcagcg gtcgacctcc
     5761 tcctcgacgg acggcagcgg cttgaggtcc ttgaggtgcg cgcgcgggtc ggcgttgacg
     5821 acgacgccgg ggttgaggag gccggccggg tcggcgagct ccttgacgcg cttcatgacg
     5881 gcgtacgcct cgggccccca ctccgtctcc acgaacggcg ccatgttccg gcccgtgccg
     5941 tgctcggcct tcagcgcgcc gtcgtagcgc ccgacgacga ggcggacgac gtcgtccatg
     6001 aagcgcgcgt actggtcgac ggcacggcgc tcacggaacg attgcgagag gacgaagtgg
     6061 aggttcccgt cgcgtgcgtg cccgaagatg atcgcgtcgg cgtagccgtg cctggcgaag
     6121 aggcccgtga ggtccgtcgc ggcgtccgcg aggcggtcca cggggaaggc gacgtcctcg
     6181 atgatcaccg tcgtcccgct cttcctcacg gcgccgaccg acgggaacat ccccttcctc
     6241 acgtgccaca ggcgcgcctg ctccgcggcg tcgtgcgtga agcgcgcggg ctcggcgagg
     6301 ctcaggccgg cagccgcgtc cgccgcggcg cgcccgatct cctcgcgctc gtcctcgcgc
     6361 gcggcctgga attcggcgag gaggccggcg gcgccggggg gcagcgcgcg gagagccggg
     6421 gggacgcccg gctcgttctc gaccgagcgc agggccgccc ggtccatgac ctccagcgcc
     6481 ttcgcgcccg ccgcgttgag cgggacgatg gcggagcagg cggcgtggag atcggggaag
     6541 aggagaaggc ccgtgacctt cacggggagg tcgggcacgg tggcgaggac cgcctccgcg
     6601 acgaacgcga gcgtcccctc cgaccccacg aggaggcgcg cgaagacgtc caccgggtgc
     6661 tcgaagtcga ggaacgcgtt gagggcgtaa ccggtcgtgt tcttcgtgcg gtactttgcg
     6721 cggatgcgcg cggcgagcgc cgcgtcggaa agtacgcgcg cacggaggtc ggcgaggccc
     6781 tgccagagcg ccggctcctt cgagcggagg acgtcgtccg cctcggggcg cgccgtgtcg
     6841 atcgccgtgc cggacggcag gacaaaggtc agcgactcga gcgtgtgata cgcgttctgc
     6901 gtgacgccgc agcacatgcc gctcgcgttg ttcgcgacga tgccgccgag cgtgcacgtc
     6961 gcgatggagg ccgggtcggg cccgatcttc cggccgaacg gcgcgagcgc gcggttcgcg
     7021 tgggcgccga tcacgcccgg ctggacgcgg atgcgcgcgc cgccgtcctc gaccgtgagg
     7081 ccgcggaagc cgcgcgccgt ctcgacgagg acgccgtccg tgacggactg gcccgagagg
     7141 ctcgtcccgg ccgcgcggaa gacgagcggg acgccgtggg cgcgcgagac ggcgaagagg
     7201 cgccggacgt cgtcggcggt cctcgcgtgc acgacggcct gcgggacgag ccggtagaag
     7261 ctcgcgtcgg aggcccacgc gatccggtcg atcgcgcgcg tgaggacgcg gtcctgtccg
     7321 atggccgcag cgagggcggc ggcgagagcg gggtccgtcg cgtccatggc gggtgcgatc
     7381 gtaatcgttc ctttgctacg ctggccgcga tgggccgttc gctcctctcg aaggtctggg
     7441 acctccacgc cgtccgcaag ctcccgacgg ggcagacgca gctcttcgtg ggcctgcacc
     7501 tcgtccacga ggtgacgagc ccgcaggcct tcgcgatgct gaaggagaac ggctggaagg
     7561 tcgcgttccc gtcgcgcacg gtcgcgaccg tggaccacat catcccgacc gacagcctcg
     7621 cgcgcccttt cgccgacgcg caggccgagg agatgctcgt ctcgctccag gagaactgcc
     7681 gcacgttcgg cgttccgctc cacgacctcg gcgcgccggg acaggggatc gtccacgtca
     7741 tcggccccga gctcggcctc acgcagccgg ggatgacgat cgcgtgcggc gacagccaca
     7801 cgtccacgca cggcgcgttc ggcgcgctcg cgttcgggat cggcacgtcc caggtgcgcg
     7861 acgtcctcgc ctcgcagtgc ctcgcgatgg acccgatgag gctccgccgg atcgacgtcg
     7921 cggggcgcct cgcgcccggc gtctacgcca aggacgtcat cctcgcgatc atccggacgc
     7981 tgggcgtcaa ggcgggcgcc ggctacgcgt acgagtacgg cggcgcggcc gtggacgcga
     8041 tgtcgatgga cgagcggatg acgctctgca acatgtcgat cgagggcggc gcgcgcgcgg
     8101 gctacgtgaa ccccgacgcg acgacgttcg cgtacctgaa gggccggccg gcggcgccgg
     8161 agggcggcgc ctgggaccgc gccgtcgcgt ggtggtcgtc gatggcgtcc gacgcggacg
     8221 ccgcgtacga cgaccgcgtg acgctggacg gaggagcgct cgaaccgcac gtgacgtggg
     8281 ggatcaaccc ttcccagagc acggggatct ccgggcgcgt gccgaaggcg gccgacgccc
     8341 ctctcgaggg ccgcgcccag gtcgccgagg cgctcgagta catgggattc gcaggcggcg
     8401 agccgatcgc ggggacgaag atcgacgtcg cgttcatcgg ctcgtgcacg aacgggcgcc
     8461 tctcggacct gcgcgaggcc gcgcgcgtcg tgaagggccg gaaggtcgcg aagggcgtca
     8521 aggcgctcgc ggtcccgggc tcgaatgcgg tcaggctggc cgcggaaaga gagggcctcg
     8581 acgccgtctt ccgcgacgcg ggcttcgagt ggcgcggcgc gggctgctcg atgtgcctcg
     8641 cgatgaatcc cgaccggctc gaggggcggc aggtctgcgc gtcgtcctcg aaccgcaact
     8701 tcaagggccg gcagggcagc cccacgggcc gcacgctcct catgagcccg gccatggtgg
     8761 cggcggcggc cgtcgcgggc gaggttgccg acgtgcggac gctgatcggg ggcgcgcgat
     8821 gagcctgccc gcgatccgcc gcgtgtcggg gacggcgcgc ccgctcctcg gcgacgacat
     8881 cgacacggac cggatcatcc cggcgcgttt cctcgtctcc gtcacgttcg acggcctcgg
     8941 cgcgcacgcg ttcgaggacg accggaaagc ggcgcgggcg cgcggcgagg tccacccgtt
     9001 cgacgacgcg cgcttctccg gcgcgtcgct cctcctcgtg aaccgcaact tcggctgcgg
     9061 gtcctcccgc gagcacgcgc cgcaggcgct cctgcgcggc gggatcaagg cggtcgccgg
     9121 cgagtcgtac tccgagatct tcttcgggaa ctccgtcgcg atcgggctgc cgtgcgtgac
     9181 gctgccgcac gacgcgatgg cggcgctgca ggagctcgtc gcgcgggacc ccgcgctgtc
     9241 gctcgcgctc gacctcgaga cgaagacgct cgcggccggc gaggcgacgt tccccgtgca
     9301 gatgcccgag aacgcccggc aggcgttcct ctccgggcac tgggacggca cgtcgatgct
     9361 cctcgaccgc ttcgaggcgg tcgaggagct ggcgaagcgc ctgccctacg tggccgggtt
     9421 ctgagcgttc tgcatcaggc ccggtccacg cggcagacga agcaaccggg cttcgcgttg
     9481 tggacgtgga ggtaggccgc cgacgggttc gcgaagagcc tcgagatcgc ctcctcgaga
     9541 tccgagcctt cccggacttc ggcgcccacc atcagtccct cggcgtcgta cgcgcgcacg
     9601 gagaggaggc ggtggcggag catcggcggg acgctgcccg gcgcgccggc ccacgccttc
     9661 accgcgctct tccggacgta gatcggcccc gaggcccggt agggcgacgg cgtcgcgtga
     9721 tgctcccagg gcaggaggag cacgtcttcg ccgagctcgg catcctggag gctgacgcgg
     9781 cacggatagc ccggcttcgc gtcgacgacg cggcggcgca ccccgcgcgc ggagagcgcg
     9841 cggtcgtcga ggtcgaagag aggctggaac gggtcggggg cgaggccttc gatccggaag
     9901 ctcatggcgg tcctcccggg ctcaagatgc ggccgccacg cctccgatgc gtcccggttc
     9961 ttgcggtcaa accgaagatg gaggcgccgg cgcgggcgcc tccatcttcc cggttggttc
    10021 ggcctaaggc ctgaacgtcg cgatcacccc ggcgtggtcg gaggcgtggg gcgggacggg
    10081 gaggctcccg aagggaagaa tgaccaattc ggcgccgatg gggttcatgc ccttcgagaa
    10141 gaacaggtcg atgcgctcgt acggactgct gtcggcgaag aaaggcggcg gcggaaactg
    10201 gtcctcgagg tagtagggcc atgtcagccc gctggcgcct ccgtacacct tgacccagac
    10261 gtcctcgtaa ccggccgcct cgatgagatc gaccgtcggc gttgagtcga tgaacccgcc
    10321 gtggttcgcg tcggagttga agtcgccgca gatcacgacg ggcacctgca ggttgcgcag
    10381 cgcgtggagg agttcctgtg cctgcgccac ctggacgtcc gtcgcctccg aaacgccaag
    10441 gatcgggctc tggaggtgcg tcgtgacgac acggaactgc ttgtttccga cgtgcacgtc
    10501 tgcggtgatc cagcccgccg ggacggtgag gccggcgaac aggaacgcgg cggagaaggt
    10561 gttcgcatgg acgtttgaga ggtggaactg cggaggtttg aggtcggccc ggaccaggag
    10621 cgcgttccgg tcggtgaagc ggagggcgcc gagtgtggcc cccggcacgg cgatgtcgaa
    10681 aaggtcgttc acggcgacga ctttgtaggg agctccgagg tcctggagag cggagacgag
    10741 ggtgtccagc gagtcgctga ggggttccgt ggccttgtcg ggcgcgggcc ccgttctcca
    10801 cagcgcggct tcctgcacgg cgacgaggtg tggcttccgg tacgcgatct ccgtcgccat
    10861 ggcggtgacg cggtacggga tggcgctcgc gatcaattcg taatacgtga gatcgacggc
    10921 cgcctcgagc ggaaggagcg ggatcggcag gagggccgcg acgacgtacg tcaggtccgt
    10981 cccgtcgtcc atgttctgcg tcatgatcga gacgggcatg ttgttatccg gcttggcggc
    11041 gtcggtcggg aaggcgaggg cggcgagggc aaaaacagca agggccgcga gttttcgcag
    11101 catgactcct ccttgctggc tcgggggcgc tccggaggtc aggcatgcgt ccacacggga
    11161 caccggagcc gacctcgggt cagtgttcaa cacatgggag cgcggtggcg gcccggcatt
    11221 gatgcagctg aatcaggcgg ctggcggcgc cggtgctatc ccgccgcccg ctccttccgc
    11281 accatctcca ggaacgcgtg ggcggcgggg ccgaagttac cggcggcgcg gtagacgagg
    11341 cgcaggtcgc gcgggcggcg cagctcgggc agcgtcgtcg ccgcgaggcg gccggcctcg
    11401 atttcggaga gcgccgtgcg gcgggggagg agggcgacgc ccatcccgcg ctcgaccgcg
    11461 cgcttgacgc cgtcgaggct gggcagcgag accttgatgt tcagcgcgac gccgcgctcg
    11521 gagtacgtgc ggagcacgcg ctcgcgcgcg ggcgaggggt cgctgtgcgc gatgacggtc
    11581 tcgcggccga attcctccag cgtgacgcga tgcttcttcg cgaacgggtg cgaggggtgc
    11641 gagaggacga cgagctcgtc ggaggcgagg acgacggtct tcagcccctc ctccggcggc
    11701 acgaaggcga cgacgccgaa gtcgagcgcg accgcgagga cctcggcccc gaggaggcgc
    11761 gagggcgagc gcttcacctc gaactcgacc cccgggtgcg tctcgcggaa gcggccgatg
    11821 agcgggatca cgacctcgag gcccgcctcg ttcgcgccga tcacgacgcg cccgcgcttg
    11881 acgtccttga ggtcgcgcac ggcccgctcg gcctcgtccg cgacggcgag gacgcgctcg
    11941 gcgtaaccga ggagggtctt gccggcctcc gtcagccgca cgtcgtgcgt cgtccggtcg
    12001 aacaggtcgg ccttcagctc gtcctcgagc cgccgcacgg cctggctgac ggccgactgc
    12061 gtccgaaaca ggcgcacggc cgccttcgag aagcccctct cctcggcgac cgtcagaaag
    12121 acccgcaggg cagctacttc cataactttt gctaataata gaagaagaat tatgagtttg
    12181 gagaatttct tcgaataggg tccaatttgc gccgtgattc acagcgtccg caccgccgtc
    12241 cctgtccttc tgctttcgaa ggagatttct ctcgtcctta cggagagaca ggcggcgggc
    12301 taacggggat cacgaacaca ccgagccagc cgccccgcag ccgcggggct ttttttttgg
    12361 gtccggattc atcgtgatgg aggccagggg aaacgtgaag agcgacgcga tcaagaaagg
    12421 cccggcgcgg gctcccgcgc gcgcgatgct gcgcgcgacg ggcctgaccc cggccgatct
    12481 cgagaagccg ctcgtcgcgg tcgtccaccc gtggacggac ctcggcccgt gcaacttctc
    12541 gctgcgcgcg ctcgccgagg aggtcaaggc cgggatccgc gaggccgggg gcacgccgtt
    12601 cgagttcggg acgatcgcga tctccgacgg catctccatg ggcaccgacg ggatgcgcgc
    12661 ctcgctcgtc tcgcgcgagg tcatcgcgga ctcgatcgag ctcgcggttt ccggccacgc
    12721 gctcgacgcc gtggtcgccc tctgtggctg cgacaagacg atcccgggcg ccgcgatggc
    12781 gctcgcccgt ctcgacatcc cgtccgtcgc cctctacggc ggctcgatcg agcccgggaa
    12841 ggtcgcgggc agggacgtca cgatccagga cgtcttcgag gcggtcggcg cgcacgcggc
    12901 ggggacgatc tcggatcaga aactctgcga cgtcgaagcc ggcgcatgcc ccggggcggg
    12961 cgcgtgcggc ggccagttca cggcgaacac gatggcgacg gcgctcacgt tcctcggcct
    13021 ctcgccgatg ggcgcgaacg acgtcccggc gaccgacccc gcgaaggcgg gcgtggcccg
    13081 agcggtgggc cgcctcgtga tggaccgcct gaaggacggg ctgacgcctt cgaagatcct
    13141 gacgcggcag gccttcgaga acgcgatcgc ctccgtcgcc gccacgggcg gctcgacgaa
    13201 cgccgtcctg cacctcctcg cgatcgcgcg ggaagcgggc gtgccgctcg tcctcgacga
    13261 cttcgattcg atctcggcgc ggacgccgct cttcgtcgac ctcaagccgg gcggccgctt
    13321 cacggcgccc gacctcgcgc gcgcgggcgg ctcgcgtctc gtcgcgcggc ggctcgtgga
    13381 ggcgggcctc gcaaagaacg cgaggaccgt ctcgggccgg actctctacg aggaagccca
    13441 ggacgccgtc gagacgccgg gccaggacgt ggtccgcccc gtgtcgaagg cgctcaagcc
    13501 gcacggcggg ctcgcgatcc tgcgcggcac gctcgcgccc gagggcgcgg tcgtgaagct
    13561 cgcggggcac gagcggatgc gccacaccgg ccccgcgcgc gtgttcgacg gcgaggccgc
    13621 tgcgttcg
//
LOCUS       DAOMFY010000087        13312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1436_length_13312_cov_7.554877, whole genome shotgun sequence.
ACCESSION   DAOMFY010000087 DAOMFY010000000
VERSION     DAOMFY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1436_length_13312_cov_7.554877"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLB01_09015"
     CDS             <1..238
                     /locus_tag="PLB01_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQQ77480.1"
                     /translation="ADRIAARVASANERLGVAVARLEALSPLRVLARGYAIVTKEGEA
                     SPVTDAEALIPGDSVRLRLARGRAAARILSTETE"
     gene            235..528
                     /gene="xseB"
                     /locus_tag="PLB01_09020"
     CDS             235..528
                     /gene="xseB"
                     /locus_tag="PLB01_09020"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQQ77481.1"
                     /translation="MTKTKALPKNLSFEKALERLQEIVSELEDPEKGLEASLELFEEG
                     VALSRFCRSRIDEIQKRVDVVLKETPEGLATGPLDEELEDGDEDDDDDEDAAR"
     gene            525..1448
                     /locus_tag="PLB01_09025"
     CDS             525..1448
                     /locus_tag="PLB01_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQQ77482.1"
                     /translation="MTGLRPSRALPAVLEARRRGIEAALSSVLPREGTEPRELVAAMR
                     YSVLGGGKRFRPLLFFAGAEAGPERLPEDAGDAAAALELLHTYSLVHDDLPCMDDDDL
                     RRGQPTCHVKFGEATALLAGDALQTLGMEILATRPAGEEWAVRRARAVAMTARAIGVE
                     GMAGGQALDLAATGSPVPPEERAQRLKKIHALKTGRLIRLSVELGALHAGADDAVLAA
                     IGRFGDALGLLFQIADDLLDVTQDSATLGKTAGKDAAQDKLTYPAVFGLEGALRERDR
                     ALEEAHAAAAEVEGPGGLLASLADYVAGRDR"
     gene            1488..3965
                     /locus_tag="PLB01_09030"
     CDS             1488..3965
                     /locus_tag="PLB01_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQQ77483.1"
                     /translation="MRWKSGVRERRAAAVLGIVSLAASVALFVWSAGAESRALAAAGA
                     ACDADATRAARAVGSLLAFRDADGALAAVRETIRDRRLVSTVVLDPAGHLNAAHAVPI
                     PGLSHVSRAEVFFEDRRVGSIVLGFSLEEEGAESAKERVLALVAAACLALVGLSALVA
                     AALGQVRSRIKSVSAEHARIAVEREILAARAAEMETRLDLERRDRRFAESALSVRDLF
                     EIERTGILEMIARGEQLPSILSSLAALVEHRRPNSQCIVAPLRQGVLPVGSAGDAATP
                     FGGGTGPVTIGSTAPPLALAAEMRSPVVTRDVPGDPLWESGRELFASHGVKACWSVPI
                     EAGTGTVFGTLSLLLPEPAAPGTSDIALLENAARLAAIAIEQRQLSDQLAFQARYDRL
                     TNLPNRLYLEEALRAAVSRAARRNERVTLFFVDLDRFKRVNDTFGHGGGDALLAQVSL
                     RLAAAVDEGDIVARMGGDEFCVMRVGPGADGYVELSRRVLESLRAPFDILGEEVIVGA
                     SVGISRYPDDAHDAMALQMHADAAMYAAKEEAGSGWHLFQPGQTFRGGDRLRLESDLR
                     RAIGSGELSLVFQPQADLAGILKGAEALVRWNHPVLGEVRPSRFVPIAEESGLVLELG
                     AWVFEEVCRRIASWRAAGLPIVPVSVNVSPVQFSGSDLVGIIEASLAAHGVPANLIRL
                     ELTETLLIKDAAATMTLLGRLKNLGVGLAVDDFGTGYSSLSYLQLLPIDELKIDQAFV
                     RELGTSEMNARAPKLVQSIVALGHSLGLVVVAEGVENAAQLEFLRRVECDRVQGYAIG
                     RPMPGEALTRLLAISSRPMELQLNSTT"
     gene            3962..4351
                     /locus_tag="PLB01_09035"
     CDS             3962..4351
                     /locus_tag="PLB01_09035"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HQQ77484.1"
                     /translation="MTGSSDDRRFGWTVGGAFAVLGVVLLWRHRTVAGAVLAALGGTL
                     LLLAAAAPVLLAAPHRGWLAFAKVLGRVNSALFLFLTFFLVLTPLALFLRLLGRDELR
                     RRGPAPASTWEPYPERNRDPKHYESIW"
     gene            4351..4509
                     /locus_tag="PLB01_09040"
     CDS             4351..4509
                     /locus_tag="PLB01_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HQQ77485.1"
                     /translation="MGKRDVLSQLWEFLKYRKKYWLMPIVFLLVLVGLLLVLAEKSVI
                     APFIYTLF"
     gene            4509..6488
                     /locus_tag="PLB01_09045"
     CDS             4509..6488
                     /locus_tag="PLB01_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006620908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HQQ77486.1"
                     /translation="MAAILGLSAYYHDSAACLLVDGKIVAAAQEERFTRKKHDAAFPR
                     NAALWCLKEAGLPLAKLDHVAFYDKPLVKFERLFETYLEYAPRGFTSFRRAMPLWIAD
                     RLWMSERLGKDLGWNGKVLFGDHHESHAASAFYPSPFEEAAVLTMDGVGEWATSSVGL
                     GRGNTLDMISEMRFPHSVGLLYSAFTYFCGFRVNSGEYKLMGLAPYGEPEYADVIREK
                     LVHVCDDGSIRLNMEYFTFATGLTMTGAGFERLFGGPAREAESKLTQREMDLARSIQD
                     VTEEIVLKMARHAKRATGARYLCMAGGVALNCVANGKLREAGIFDDLWIQPAAGDAGG
                     AVGAALSVWHRHLGMPRESAEKRGTWRGNRGKEGKISLSGIGPGDGGASIAGSAPAAP
                     RSSSFEEISSSLSLPPYEDGMAGAFLGPSYPSEDISSWLSSKGYPYRTVAREALPDAV
                     AELLAEGKVVGLLQGRMEFGPRALGGRSILGDPRSAAMQSAMNLKIKFRESFRPFAPS
                     VLREDVAAWFDLDGDSPYMLLCSDVARERRREMTDGERALWGIEKLNVPRSTIPAVTH
                     VDYSARLQTVRRETNPVYHAILSAFRDRTGCPVVVNTSFNVRGEPIVCSPEDAYRCFM
                     RTAMDALVLENHVLVREGQPPLAESGDWRREFTLD"
     gene            complement(6489..7901)
                     /locus_tag="PLB01_09050"
     CDS             complement(6489..7901)
                     /locus_tag="PLB01_09050"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQQ77487.1"
                     /translation="MTQSNGFLESVNRNFDAAGTFLDYPPGLLDQIKVCNSVYKFRFP
                     VRKEGGGYEVIEAWRAEHSHHKLPVKGGIRYSPDVNEDEVLALAALMTFKCAIVDVPF
                     GGAKGGIKVDPRKVSLEQLERITRRYTAELAKKNFIGPGIDVPAPDYGTGPREMSWIA
                     DTYAQFHPGEIDAIGCVTGKPISQGGIRGRKEATGRGVFFGVKEALADADLLKTLGLT
                     AGLAGKRVAVQGLGNVGYHAAKFLQEGGATIVALAEWEGTIVDPKGLDVDAVVKHRAA
                     TKKITGFPGATDLAAEDVLYTDCDVLVPAALENVITGANAGRVKAKIVAEGANGPVTA
                     DGDAILREKGVVVMPDMYLNAGGVTVSYFEWLKNLSHVRFGRMEKRYEEMSAARMVKS
                     IESATGKTLGDAERKLLIHGAAEEDLVNSGLEETMSVAFQQIRDVQRRNPKVPDLRTA
                     AFISAIDKIAVSYMELGIFP"
     gene            7954..8454
                     /locus_tag="PLB01_09055"
     CDS             7954..8454
                     /locus_tag="PLB01_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HQQ77488.1"
                     /translation="MEVGVAGVVRLDRIALHRLSKAAVSSPRLRLNRNLHAMEDPVHR
                     LLNAIEPGSYIRPHRHLHPPKAETMVVVSGRLGFLAFHDDGTVADRAVLDAAGETFGI
                     DVAPGVWHTFVSLAEGTVGFEAKAGPYVAPAGSDAAPWAPPEGDPASVRLEAGWRNAF
                     IEQAES"
     gene            8538..11669
                     /locus_tag="PLB01_09060"
     CDS             8538..11669
                     /locus_tag="PLB01_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQQ77489.1"
                     /translation="MIKLRRLLPLAMCVAVLLSAVPALGQETTAALQGVVTVKSDKSP
                     LPGVTVEALHVPTGTRFAAVTSVTGRFNILNVRVGGPYTVTAKIGGFKTETQKDVVVA
                     LGEKLELEFAMELAARAETVSVTAELEPLISPNRMGSTQAVSQDEIKALPTIRRQFQD
                     FAKTSPYVNVAASDPTQTNISVGGKNNRYNTIQIDGAVNNDLFGLSSTGTPGGQTDTQ
                     PISIDTIQEIQVAISPYDVKQGGFTGGAINAITRSGTNEFHGSVYGSSRNQNWVGSKI
                     PQAYGDAVSSPLAEFTSDQYGARLGGPIIKDTLFFFGSAERNKTSAPTNGAADGSASN
                     NFNNPANAAAFKAVLTNKYGYNPGGLGDYNTRTNSDLLLGKLDLNISSAHQLSLRYNY
                     VNALNDINGARNSSTYTFETGGYTITDKTNSGVLQINSVFGSDSFNQGRVGYQTVRDS
                     RAVPVQFPSVYICNTPNSNCTGNGTYNLAAGTERSSGANSLNQDILEVTDDFTLIKGN
                     HTITIGTHNEFFKFENLFIQDVYGTYFFNTINDLNNGNAARYQVQFANGADPRRPTQF
                     NARQWGIYAGDQWRVSNTLTLNLGLRLDIPQLPDSPSYNPLVQSTFGVDTSNVPSGQI
                     MVSPRLGFNWNPSGSGNDQIRGGIGVFAGRTPYVWISNNYGSTGIEITNLSASNVTFT
                     GDPNNPPKNFPPGTSAITVNGINPDFKFPQVLRSTLAYDRELPYGIRATVEAMFTKTL
                     QDVFYYNLGRVESGTKTFYGAPVYKQYSTQISDMIYLDNTTKGEQQNLVVQLEKRFPF
                     GLYFMGSYAYMNAKAAFEGTSSVAYSNWQFQTTDGNIYNQALTRSFWDVPNRFNIVAS
                     QAFRTGSFGHNVGLIFTAQSGQPYSILMGGNANQDGASGNDLLFVPANYNDVVWRGAG
                     APTEGQWNDYLSMTGLDKYRGRVAERNALDAPWIHTLDFHYDVTLPISVVQVQLTFDV
                     LNLINLINHNAGLLRYVSNQTYTALNYSGIDAATGKPIYTVNSGALNEGRQYTTQGLR
                     SRYQLKLGGRVSF"
     gene            11874..13046
                     /locus_tag="PLB01_09065"
     CDS             11874..13046
                     /locus_tag="PLB01_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQQ77490.1"
                     /translation="MSLATETPAIPPVSVLGDHERELAEGVYDFAVESIAPQSARMDR
                     DNVMDPDLIRKFFDLDLMGIEIPEEFGGLGANFTTSIAIIEALARVDAACAVVVDVQN
                     TLVNNAIKRWASPAIQRKYFPQLAKQKVASYALSEAGSGSDAFALATTAAQKGDRWVL
                     NGEKLWITNGLEAELFLVFANVDKAKGYKGITAFLVESQWKGFTKGKKEDKLGIRASS
                     TLALAFEDLEVPAENVVGNVGEGYKIAIETLNEGRIGIGAQMVGIARAALDYTTTYVK
                     ERRQFGQPLAQFQAVQHELARMDMELECARLAVYNAARLKEAGLPFAREGAIAKLKGS
                     EVAEMCTSKGLELLGGYGYVKDYPLEKMYRDAKIGKIYEGTSFMQLNTIAKDVLKR"
BASE COUNT         2119 a         4605 c         4472 g         2116 t
ORIGIN      
        1 ggccgaccgg atcgcggcgc gcgtggcctc cgcgaacgag aggctcggcg tcgccgtggc
       61 gcggctcgag gccctgagcc cgctgcgagt cctcgcgcgc ggctatgcga tcgtgacgaa
      121 ggagggggag gcgtcgccgg tgacggacgc cgaggccctc atccccggcg acagcgtccg
      181 tctgcggctg gcccgcggtc gcgccgccgc ccgaatcctc tcgacggaga ccgaatgacc
      241 aagacgaagg ccctgccgaa gaacctctcg ttcgagaagg ctctcgaacg gctccaggaa
      301 atcgtgtccg agctcgagga ccccgagaag ggcctcgagg cgagtctcga gctgttcgag
      361 gagggggtcg cgctgtcgcg gttctgccgc tcgcgcatcg acgagatcca gaagcgcgtc
      421 gacgtcgtcc tgaaggagac gcccgagggg ctcgcgacgg gccccctgga cgaggagctc
      481 gaggacgggg acgaggacga cgacgacgac gaggacgcgg cccgatgacg ggcctgcgcc
      541 cctcgcgggc gctgccggcc gtgctcgagg cgcggcggcg ggggatcgag gcggccctct
      601 cgagcgtcct gccccgcgag ggcaccgagc cccgggagct cgtcgccgcg atgcgctaca
      661 gcgtcctcgg aggcggcaag cgtttccgtc cgctgctctt cttcgcgggc gccgaggccg
      721 gcccggaacg gctgccggag gacgcggggg acgccgcggc ggcgctcgag cttctccaca
      781 cgtattcgct cgtccacgac gatctcccgt gcatggacga cgacgacctg cgccgcggac
      841 agccgacctg tcacgtgaag ttcggcgaag cgaccgcgct cctcgcgggc gacgcgctcc
      901 agacgctggg catggagatt ctcgcgacgc gtcccgcggg cgaggaatgg gccgtgaggc
      961 gcgcgcgggc cgtggcgatg acggcgcggg cgatcggcgt ggaaggaatg gcgggcgggc
     1021 aggcgctcga cctcgcggcc acggggagcc ccgtgcctcc cgaggagcgc gcccagcgcc
     1081 tcaagaagat ccacgcgctg aagaccggcc gcctcatccg gctctccgtc gagctcgggg
     1141 cgctgcatgc cggcgccgac gacgcggtgc tcgcggcgat cggccgcttc ggggacgcgc
     1201 tcggcctcct cttccagatt gccgacgacc tcctcgacgt cacgcaggac tcggcgacgc
     1261 tcggcaagac cgcaggcaag gacgccgcac aggacaagct cacctatccg gccgtgttcg
     1321 gcctggaagg cgcgctccgc gagcgcgacc gcgcgctcga ggaggcgcac gccgccgccg
     1381 ccgaggtcga agggcccggc ggtctcctcg cgtcgctcgc ggactacgtc gcgggccgcg
     1441 accgctgacg tgccggcgcc gggcatccga gcgttacagt acgccgcgtg aggtggaagt
     1501 cgggcgtccg ggaacgaagg gccgcggccg tcctcgggat cgtgtccctg gcggcgtccg
     1561 ttgcgctctt cgtctggtcg gcgggtgccg agtcgcgtgc gctcgcggct gcgggagccg
     1621 cctgcgatgc ggacgcgacg cgcgccgcca gggccgtcgg ctcgctgctc gcgttccggg
     1681 atgcggacgg cgcgctcgcg gcggtccgcg agaccatcag ggaccgccgc ctggtttcga
     1741 ccgtcgtcct cgatccggcg ggacacttga acgcggcgca cgcggtcccg atccccggcc
     1801 tgtcccacgt ttcgcgtgcc gaggtgttct tcgaggaccg gcgcgtgggt tcgatcgtcc
     1861 tcgggttctc gctcgaggag gagggcgccg agtccgcgaa agagcgcgtc ctcgccctcg
     1921 tcgcggccgc gtgtctcgcg ctggtcgggc tctccgcgct cgtcgcggcc gctctcggac
     1981 aggtccggtc gagaatcaag agcgtgtccg cggagcacgc gcggatcgcc gtcgagcggg
     2041 agatcctcgc ggcgcgggcg gccgagatgg agacgcgcct cgacctcgag cggcgggaca
     2101 gacgcttcgc ggagagcgcg ctttcggtcc gcgacctctt cgagatcgag aggacgggga
     2161 ttctcgagat gatcgcccgc ggtgaacagc tcccgtcgat cctctcgagc ctcgccgcgc
     2221 tcgtcgagca ccgccggccg aattcgcagt gcatcgtcgc ccccttgagg cagggggtgc
     2281 tgccggtcgg ctcggcgggt gacgccgcga ccccgttcgg aggcggaacc ggccccgtca
     2341 cgatcggctc gacggcaccg ccgctcgccc tcgccgccga gatgaggagc ccggtcgtca
     2401 cgcgcgacgt tcccggggac cccctgtggg agtcgggccg cgagctcttc gcctcgcacg
     2461 gggtcaaggc gtgctggtcc gtaccgatcg aagcggggac cgggaccgtc ttcggaacgc
     2521 tcagcctgct tcttcccgaa ccggcggcgc cggggacctc ggacatcgcc cttctcgaaa
     2581 atgcggcgcg cctcgcggcc atcgcgatcg agcagcggca gctctcggac cagctcgcgt
     2641 tccaggcacg ctatgaccgg ctcacgaacc tgccgaaccg cctctatctc gaggaggcac
     2701 tccgcgccgc cgtctcgcgc gccgcgcgcc gcaacgaacg ggtgacgctg ttcttcgtgg
     2761 atctcgacag gttcaagcgg gtgaacgaca cgttcggtca cggcggcggc gacgctcttc
     2821 tggcgcaggt gtcactccgc ctcgcggcgg cggtggacga gggcgacatc gtcgcgcgaa
     2881 tgggcggcga cgaattctgc gtgatgcgcg tcggtccggg ggcggacggc tacgtcgagc
     2941 tttcccggcg ggtgctcgag tcacttcgcg cgccgttcga catcctcggt gaggaggtca
     3001 tcgtcggcgc cagcgtcggt atcagccggt atccggacga cgcgcacgac gcgatggcgc
     3061 ttcagatgca tgcggacgcg gccatgtacg ccgcgaagga ggaggcgggg agcggctggc
     3121 acctcttcca gcccggccag accttccgcg gaggcgaccg cctcaggctc gagagcgacc
     3181 ttcggcgggc gatcggcagc ggcgagctga gcctcgtgtt ccagccccag gcggacctcg
     3241 ccggcatcct gaaaggcgcg gaggcgctcg tgcgctggaa tcaccccgtt ctgggcgagg
     3301 tccggccgtc ccggttcgtg ccgatcgccg aggagagcgg cctcgttctc gagctcggcg
     3361 cctgggtctt cgaggaagtc tgccgccgca tcgcgtcgtg gcgggcggcc ggtctgccga
     3421 tcgtgccggt gtcggtcaac gtctctcccg tgcagttctc ggggtccgac ctcgtgggga
     3481 tcatcgaggc gagcctggcg gcgcacggcg ttccggcgaa cctgatccgt ctggagctca
     3541 ccgagacgct cctcatcaag gacgccgcgg ccacgatgac gcttctgggc cggctcaaga
     3601 acctcggcgt cgggctggcc gtcgacgact tcggaaccgg ctactcgtcg ctgtcgtacc
     3661 tgcagctcct gccgatcgac gagctcaaga tcgaccaggc gttcgtccgg gaactgggga
     3721 cctccgagat gaacgcgcgg gcgccgaaac ttgtccagtc gatcgtcgcg ctcgggcaca
     3781 gcctcggcct cgtcgtcgtc gcggagggcg tcgagaacgc cgcgcagctc gagttcctga
     3841 gacgcgtcga gtgtgaccgc gttcagggct acgcgatcgg gcgccccatg cccggcgagg
     3901 cgcttacgag actcctcgcg atctcgagtc ggccgatgga gttgcagcta aactcgacga
     3961 cgtgaccggc tcgagcgacg accgcagatt cggctggacc gtgggcggcg ccttcgcggt
     4021 cctgggcgtt gttctcctct ggaggcatcg gacggtcgcg ggggccgtgc tggccgccct
     4081 cggcggaacc ctgctgctcc tcgccgccgc cgcgccggtc ctgctcgccg cgccgcaccg
     4141 cggctggctc gcgttcgcga aggtcctcgg gcgcgtgaac tcggctctct tcctcttcct
     4201 gacgttcttc ctcgtcctca cgcctctggc gctcttcctc cggctcctgg gccgggacga
     4261 gctgcggcgg cggggcccgg cgcccgcgtc cacgtgggag ccgtatccgg aacggaaccg
     4321 cgacccgaag cactacgagt cgatctggtg atgggcaagc gcgacgtcct ctcgcagctc
     4381 tgggagttcc tcaagtaccg caagaagtac tggctcatgc cgatcgtgtt cctgctcgtt
     4441 ctcgtcgggc ttctcctcgt tctggccgag aagagcgtca tcgcgccctt catctacacg
     4501 ctgttctagt ggcggcgatc ctcggcctct cggcctacta ccacgactcg gccgcgtgtc
     4561 ttctcgtgga cgggaagatc gtcgcggccg cgcaggaaga gcgcttcacc cggaagaagc
     4621 acgacgccgc cttcccgcgg aacgcggcgc tgtggtgcct gaaggaggcg ggtcttccgc
     4681 tcgcgaagct cgaccacgtc gcgttctacg acaagccgct cgtgaagttc gagcggctct
     4741 tcgagacgta cctcgagtac gccccgcgcg gattcacgtc gttccgcagg gcgatgcccc
     4801 tctggatcgc ggatcgcctc tggatgagcg agaggctcgg gaaggacctc ggctggaacg
     4861 ggaaggtcct cttcggcgac caccacgagt cccacgcggc ctcggcgttc tatccgtccc
     4921 ccttcgagga ggccgccgtc ctcacgatgg acggcgtggg cgagtgggcc acgtcctcgg
     4981 tcggactcgg ccgtggaaac acgctcgaca tgatctccga gatgcggttt ccgcactcgg
     5041 tcggcctcct gtactcggcg ttcacctact tctgcggctt cagggtgaac agcggggaat
     5101 acaagctgat gggcctcgcg ccctacggcg aaccggagta cgcggacgtg atccgcgaga
     5161 agctggtgca cgtctgcgac gacggctcga tccggctcaa catggagtac ttcacgttcg
     5221 cgaccggcct cacgatgacg ggcgcgggct tcgaacggct cttcggtggg ccggcgcgcg
     5281 aggccgagtc gaagctcacg cagcgcgaaa tggacctcgc ccggtcgatc caggacgtga
     5341 ccgaggaaat cgtcctgaag atggcccggc acgcgaaacg ggcgacgggc gcgagatacc
     5401 tctgcatggc gggcggcgtc gcgctgaact gcgtcgcgaa cgggaagctc cgcgaggcgg
     5461 ggatcttcga cgacctctgg atccagcccg ccgcgggcga cgcgggcggc gccgtgggcg
     5521 cggcgctctc ggtctggcac cgccacctcg ggatgccgcg cgaaagcgcg gaaaagaggg
     5581 ggacctggag agggaaccga ggaaaagaag ggaagatttc tttgagtgga atagggccgg
     5641 gcgacggcgg cgcatccatt gccggcagcg cgccagcggc gccgcgctct tcatcattcg
     5701 aagaaatctc ttcctctctc tctcttcccc cctatgaaga cggcatggcg ggcgcgtttc
     5761 tcggcccctc ttacccttca gaagatatct cttcctggct ctcttcgaag ggctacccgt
     5821 accggaccgt cgcccgcgag gcgctcccgg acgccgtcgc ggagctgctg gcggagggga
     5881 aggtcgtcgg cctgctgcag gggcggatgg agttcgggcc gcgggccctc ggaggccgga
     5941 gcatcctcgg cgacccgcgc tcggccgcga tgcagtccgc catgaacctg aagatcaagt
     6001 tccgcgagtc gttccgcccg ttcgcgccct cggtccttcg agaggacgtc gcggcgtggt
     6061 tcgacttaga cggggactcg ccgtacatgc tcctttgctc ggacgtggcg cgcgagcggc
     6121 gccgcgagat gacggacggc gagcgcgcgc tctgggggat cgagaagctg aacgtgccgc
     6181 gctcgacgat ccccgccgtg actcacgtcg actactccgc gcgtctccag accgtccggc
     6241 gggagacgaa cccggtctac cacgcgatcc tctcggcgtt ccgagaccgg accggctgcc
     6301 ccgtcgtcgt gaacacgtcg ttcaacgtgc gcggcgagcc gatcgtctgt tcgccggagg
     6361 acgcgtaccg ctgcttcatg cggaccgcga tggacgccct cgtcctcgag aaccacgtcc
     6421 tcgtccgcga gggccagccc ccgctcgcgg aaagcggaga ctggcgccgc gaattcacgc
     6481 tggactgatc aggggaagat cccgagctcc atgtagctga cggcgatctt gtcgatcgcc
     6541 gagatgaagg cggccgtgcg caggtccggc accttcgggt tgcggcgctg gacgtcgcgg
     6601 atctgctgga aggcgacgct catcgtctcc tcgagccccg agttgacgag gtcctcctcg
     6661 gccgctccgt ggatgaggag cttgcgctcg gcgtccccga gggtctttcc ggtcgcgctc
     6721 tcgatcgact tgaccatgcg cgcggcgctc atctcctcgt agcgcttctc catccggccg
     6781 aagcgcacgt gtgagaggtt cttgagccac tcgaagtagc tcaccgtgac accgcccgca
     6841 ttgaggtaca tgtcgggcat cacgacgacg cccttctccc gcaggatggc gtcgccgtcg
     6901 gccgtgaccg ggccgttcgc gccttcggcg acgatcttcg ccttcacccg gcccgcgttc
     6961 gcgcccgtga tgacgttctc gagcgccgcc gggaccagga cgtcgcagtc cgtgtagagc
     7021 acgtcctcgg cggcaaggtc cgtcgcgccg ggaaagccgg tgatcttctt cgtcgcggcg
     7081 cggtgcttga ccacggcgtc cacgtcgagg cccttcgggt cgacgatcgt cccctcccac
     7141 tccgcgagcg cgacgatcgt ggcgccgccc tcctggagga acttcgcggc gtggtagccg
     7201 acgttgccga gcccctggac cgcgacgcgc ttgccggcga ggccggccgt caggccgagg
     7261 gtcttcagga gatcggcgtc ggcgagcgct tccttgacgc cgaagaacac gccgcggccg
     7321 gttgcttcct tgcggccccg gatgccgccc tggctgatcg gcttgcccgt gacgcacccg
     7381 atcgcgtcga tttcgccggg gtggaactgg gcgtacgtgt ccgcgatcca cgacatctcg
     7441 cgcgggccgg tgccgtagtc tggggcgggg acgtcgattc cgggaccgat gaagttcttc
     7501 ttggcgagct cggccgtgta tcggcgcgtg atgcgttcga gctgctcgag agagaccttg
     7561 cgcggatcga ccttgatgcc gcctttcgcc ccgccgaagg gcacgtcgac gatcgcgcac
     7621 ttgaacgtca tgagggccgc gagcgcgagc acctcgtcct cgttcacgtc gggggagtac
     7681 cggatgccgc ccttgacggg cagcttgtgg tggctgtgct cggcacgcca cgcctcgatg
     7741 acttcgtagc ccccgccttc cttccggacg gggaaccgga acttgtagac gctgttacag
     7801 accttgatct ggtccagaag gcccggcgga tagtcgagaa aagtgcctgc ggcgtcgaag
     7861 ttccggttca cgctctccaa gaatccgttg gactgggtca tcaaaacctc cggttgaccg
     7921 agtttagtgg agtcgatata ggatctggca ccaatggaag tcggggtcgc cggcgtggtg
     7981 cgactggatc gcatcgcact gcaccgcctg agcaaggcgg cggtttcgtc cccccgcctc
     8041 cgcctgaacc ggaaccttca cgcgatggaa gatcctgtgc atcgccttct caacgcgatc
     8101 gagcccggga gctacatccg gcctcaccgc cacctgcatc cgccgaaggc cgagacgatg
     8161 gtcgtcgtgt ccggccggct cggattcctt gcgttccacg acgacggaac cgtcgcggac
     8221 cgggcggttc tcgacgccgc cggcgagacg ttcggcatcg acgtcgctcc gggcgtctgg
     8281 cacacgttcg tgtccctcgc cgaggggacc gtgggcttcg aggcgaaggc cggaccctac
     8341 gtggcgcccg cgggaagcga cgccgcgccc tgggcgccgc ccgagggcga cccggcgtcc
     8401 gtgcggctcg aggccgggtg gcgcaacgcg tttatcgagc aggcggagtc ctgatgcatg
     8461 aagatcttac cgtgccaagc ctgttttccc ggccgtgccg ggttgatttc gtttcacatg
     8521 atggaagggg tgtgcccatg atcaaactgc gtcgtctcct gccgctcgcc atgtgcgtgg
     8581 cggtccttct gtcggctgtt cccgccctcg ggcaggaaac cacagccgct cttcagggtg
     8641 tcgtgacggt caagtcggac aagagcccgc tgccgggcgt cacggtggag gccctccacg
     8701 tcccgaccgg cacccgcttc gcggccgtca cgtcggtcac cggacggttc aacatcctca
     8761 acgtccgcgt gggcggcccg tacacggtca ccgcgaagat cggcggcttc aagaccgaga
     8821 cgcagaagga cgtcgtcgtc gccctcggcg agaagctcga actcgagttc gcgatggagc
     8881 tcgcggcgag ggccgagacg gtcagcgtga cggcggagct ggagccgctc atcagcccga
     8941 accggatggg gtcgacgcag gcggtctcgc aggacgagat caaggcgctg ccgacgatcc
     9001 gccggcagtt ccaggacttc gccaagacga gcccctacgt caacgtcgcg gccagcgacc
     9061 cgacgcagac gaacatctcg gtcggcggca agaacaaccg atacaacacg atccaaatcg
     9121 acggcgcggt caacaacgac ctcttcggcc tctcctcgac cggcacgccg ggcggccaga
     9181 cggacacgca gcccatctcg atcgacacga tccaggagat ccaggtcgcg atctcgccct
     9241 acgacgtcaa gcagggcggc ttcacgggcg gcgcgatcaa cgcgatcacg aggagcggaa
     9301 cgaacgagtt ccacggctcc gtctacggct cgtcccgcaa ccagaactgg gtcggaagca
     9361 agatcccgca ggcttacggc gacgcggtct cgagcccgct cgcggaattc acctcggacc
     9421 agtacggcgc gcgcctcggc gggccgatca tcaaggacac gctcttcttc ttcgggagcg
     9481 ccgagcggaa caagacctcg gctccgacga acggggccgc ggacggctcg gcgtccaaca
     9541 acttcaacaa cccggcgaac gccgccgcgt tcaaggccgt gctgacgaac aagtacggct
     9601 acaacccggg cggcctcggc gactacaaca cccgcacgaa cagcgacctc ctgctcggca
     9661 agctcgactt gaacatctcg agcgcccacc agctgtccct gcgctacaac tacgtcaacg
     9721 ccctgaacga catcaacggc gcgcgcaact cgagcacgta cacgttcgag acgggcgggt
     9781 acacgatcac cgacaagacg aactcgggcg tcctgcagat caacagcgtc ttcgggtccg
     9841 attcgttcaa ccagggccgc gtgggctacc agacggtgcg ggacagccgg gcggtcccgg
     9901 tccagttccc gtccgtctac atctgcaaca ccccgaactc caactgcacc ggcaacggga
     9961 cgtacaacct tgccgccggg accgagcgct cgtccggcgc gaactcgctc aaccaggaca
    10021 tcctcgaggt cacggacgac ttcacgctca tcaagggcaa ccatacgatc acgatcggga
    10081 cgcacaacga gttcttcaag ttcgagaacc tcttcatcca ggacgtctac gggacgtact
    10141 tcttcaacac gatcaacgac ctcaacaacg ggaacgccgc ccggtaccag gtccagttcg
    10201 cgaacggcgc ggacccgcgg cgcccgaccc agttcaacgc gcggcagtgg gggatctacg
    10261 cgggtgacca gtggagggtg agcaacaccc tcacgctgaa cctcggcctc aggctcgaca
    10321 ttccccagct gcccgacagc ccgagctaca acccgctcgt ccagtcgacc ttcggtgtcg
    10381 acacgagcaa cgttccgagc ggccagatca tggtctcgcc ccgcctcggc ttcaactgga
    10441 acccgagcgg gtccggcaac gaccagatcc gcggcggcat cggcgtcttc gccggccgca
    10501 cgccgtacgt ctggatctcg aacaactacg gctcgacggg catcgagatc acgaacctga
    10561 gcgcgtcgaa cgtcacgttc acgggggacc ccaacaaccc cccgaagaac ttcccccccg
    10621 gcacgtcggc gatcacggtc aacggcatca acccggactt caagttcccg caggtcctcc
    10681 gctcgacgct ggcgtacgac cgcgagctcc cgtacgggat ccgcgcgacc gtcgaggcga
    10741 tgttcacgaa gacgctgcag gacgtcttct actacaacct cggccgggtc gagtccggga
    10801 ccaagacgtt ctacggggcg ccggtctaca agcagtattc gacgcagatc tcggacatga
    10861 tctacctcga caacacgacg aagggcgagc agcagaacct cgtcgtgcag ctcgagaagc
    10921 gcttcccctt cggcctttac ttcatgggct cctacgccta catgaacgcc aaggccgcgt
    10981 tcgagggaac gtccagcgtg gcgtactcga actggcagtt ccagacgacg gacggcaata
    11041 tctacaacca ggcgctcacg cgctccttct gggacgttcc gaaccggttc aacatcgtcg
    11101 cgtcgcaggc gttccggacc ggctcgttcg gccacaacgt cggcctcatc ttcacggccc
    11161 agtcggggca gccgtactcg atcctgatgg gaggcaacgc gaaccaggac ggcgcctcgg
    11221 gcaacgacct cctcttcgtg cccgcgaact acaacgacgt cgtgtggagg ggcgcggggg
    11281 ctccgaccga gggtcagtgg aacgactacc tgtcgatgac gggcctggac aagtaccgcg
    11341 gccgcgtcgc ggagcgcaac gcgctcgacg ccccgtggat ccacacgctc gacttccact
    11401 acgacgtgac gctgccgatc tccgtcgtcc aggtgcagct cacgttcgac gtcctgaacc
    11461 tcatcaacct gatcaaccac aacgcgggtc tgctccgcta cgtctccaac cagacgtaca
    11521 cggcgctcaa ctactcggga atcgacgcgg ccaccggcaa gccgatctac accgtcaact
    11581 cgggcgcgct caacgaaggt cgccagtaca ccacgcaggg cctccgctcg cgctaccagc
    11641 tcaagctcgg cggacgcgtc tcgttctgag atcggttcaa acgctcgctt gaacggcaac
    11701 gccggggctt cggccccggc gttttttcgt tcggaagaag attttcctag aaggtgcccg
    11761 agccgggggc cgactcgtag cgcctgcggg agcgggttgc ccgcattcac tttctaggaa
    11821 gatcttcatc cggtcgtctt tcagatattc tacgaacccg gaggatttct tcgatgtccc
    11881 tcgccacaga aacgcccgcg atcccgccgg tctccgtcct cggggaccac gagcgggagc
    11941 tcgccgaggg cgtgtacgac ttcgcggtcg agtcgatcgc cccgcagtcc gcccgcatgg
    12001 accgcgacaa cgtgatggac cccgacctca tccggaagtt cttcgacctc gacctcatgg
    12061 gcatcgagat ccccgaggag ttcggcggcc tcggcgcgaa cttcacgacg tcgatcgcga
    12121 tcatcgaggc tctcgcccgg gtggacgccg cctgcgcggt cgtcgtggac gtccagaaca
    12181 cgctcgtgaa caacgcgatc aagcgctggg cgtctcccgc gatccagagg aagtacttcc
    12241 cccagctcgc gaagcagaag gtcgcctcgt acgcgctgtc ggaggccggc tcgggatcgg
    12301 acgccttcgc gctcgccacg accgcggcgc agaagggcga ccggtgggtc ctgaacggcg
    12361 agaagctctg gatcacgaac ggcctcgagg ccgagctctt cctcgtcttc gcgaacgtcg
    12421 acaaggcgaa ggggtacaag ggcatcacgg cgttcctcgt cgaatcgcag tggaagggct
    12481 tcacgaaggg caagaaggaa gacaagctcg gcatccgcgc gtcttccacg ctcgcgctcg
    12541 ccttcgagga tctcgaagtc ccggccgaga acgtcgtcgg caacgtcggc gagggctaca
    12601 agatcgcgat cgagacgctc aacgagggac ggatcgggat cggcgcccag atggtcggca
    12661 tcgcgcgcgc cgcgctcgac tacacgacga cgtacgtcaa ggagcgccgg cagttcggcc
    12721 agccgctcgc gcagttccag gccgtccagc acgagctcgc gcgcatggac atggagctcg
    12781 agtgcgcccg gctcgccgtt tacaacgcgg cgcggctcaa ggaggccggc cttccgttcg
    12841 cgcgcgaggg cgcgatcgcg aagctcaagg gctccgaggt cgccgagatg tgcacgtcga
    12901 agggcctcga gctcctcggc ggctacggct acgtgaagga ctacccgctc gagaagatgt
    12961 accgcgacgc gaagatcggg aagatctacg aggggacgtc gttcatgcag ctcaacacga
    13021 tcgcgaagga cgtcctgaag cgctgagcgg cgactccccg gtccgtccgg ccccgcgcgc
    13081 ggccctcgcc gcggggctcg cgctcctcct cggcgcggcg gcgttcctgc gctcggcggg
    13141 atggggtctc tggtcctact ggctggacga ggcgatccag gcggtcctct cccggcagga
    13201 gctcgcgaag ctgttcgcgt cgctccgtgc ggactccgcg cacccgccgc tcgcctacct
    13261 tctcacctgg gcgatcgagc gcgccggcgg gggagaagcg gcgctccgcg gc
//
LOCUS       DAOMFY010000088        13119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1455_length_13119_cov_7.872244, whole genome shotgun sequence.
ACCESSION   DAOMFY010000088 DAOMFY010000000
VERSION     DAOMFY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13119
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1455_length_13119_cov_7.872244"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3914)
                     /locus_tag="PLB01_09070"
     CDS             complement(<1..3914)
                     /locus_tag="PLB01_09070"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HQQ77491.1"
                     /translation="MIRRGLALFPAALLALAHPLAAGVGPAAQAFVPITACRAVDTRG
                     PAGPNGGPALGANEVRVFTIAGTCGVPANALGLVVNLTVVNPAAVGYAQLFAGDAPVP
                     PTTVLGFNARKTRAGFAFVALASDGSGTVALANGSTGSADYLLDVEGYFVLACTSPIL
                     VTNPATAAGAVNSPFSQTFTSSGGVGAKTYTTSSPLPAGLTLAANGTLSGTPTQSGTF
                     PITVTATDQNGCTGTGPTYTLVIACQTITVTNPGVTTGTANTPFSQTFTQSGGVGTVT
                     FSTGSALPIGMTLAASGVLSGTPTQAGGFDIVVTATDANGCTGAGATYHLVINCQTIT
                     VTNPATTTGTVNAAFSQTFTQAGAIGSATFSTVSTLPAGLALAANGVLSGTPTQKGTF
                     PIVVKVTDGNLCTGTGATYNLTINCQTIAVSNPATSTATANTPFSQTFTASNTVGTVT
                     FTTASTLPAGLTLAANGVLSGTPTQTGSFPIVVTATDANGCAGIGATYTLVVGCQTWS
                     ILPAALPQATSGVAYTATLTQSGGIGAVTYAVTSGTLPAGLGLSSGGVLSGTTALTGS
                     YPFTVTATDANGCTATRDYLLVVSCPSTPITLSPGSLAAAPANAPFPATVFSASGGAG
                     PYTFQLAGALPSGMTFSTDTLSGTPTQTGSFPITISATDAGGCAGSQDYVVTITCNGV
                     TITVAPPSLAAGPVGSPYGPITFTASGGTAPYALAEVGALPPGMTYAAGVLSGTPTQP
                     GTFPVTVTATDANGCTGQTVYSLVTTCPTIAVTNPSTTTGTAGVAFSQQFSQSGGSGT
                     ATFSTTSTLPTGLTLAADGTLSGTTSQAGTYPIVVKATDANGCTGTGATYTLVIDCQT
                     ITVTIPATTTGTEGNSFSQTFTASGILGTVTWSTASTLPAGVTLNPSTGVLSGTPTET
                     GSFPIVVKATDTNGCFGTSATYTLVLTCPTITVSGTIPALAYNTAMSTATFSQSGTTS
                     TITWSATGLPGGVAINSSTGAVTGTPTATGTFSATITATDAGGCTGSKSVTVTVAPTA
                     PSQSYTGVGNTQVYVTGVAGAPATPAVASGTALLNGVQPLGATWVSAASCSSGGTIAT
                     FDLVGRFIFTPDVAATSATCTYTVSSNTAGTPTPTTATASLTFTLSGMVWYVDNATAS
                     GTNDGRSSTPLKTMAAVGTASTGSGDFIYVAKGSGSTTGAYAMKTSQRLVGAGATLNV
                     PTVSPILTVAGSAANTPTLSGTLTLASFVNVSGIDMATTTSRAITNFNGSSYATVTGV
                     SVTARTITTTTGTAIDIQGTGNTGVMSFTSVSANGGTAGIVLS"
     gene            complement(4058..5032)
                     /locus_tag="PLB01_09075"
     CDS             complement(4058..5032)
                     /locus_tag="PLB01_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate-resistant acid phosphatase type 5
                     family protein"
                     /protein_id="HQQ77492.1"
                     /translation="MLRRLAGAALLAAAVAACGPSPAPPARTTPTAAAVLPQPPAGSL
                     EFLVLGDWGRGGNRVQRQVAGAMAEWAGKRPIRFVVSTGDNFYEDGVSSVTDRAWKGS
                     FEDVYAAPSLLRLPWIVALGNHDYRGNVEAQVEYSKSSPRWRMPARSFTVQETAGDGT
                     RVEFFVMDTTPFLATYRSFLSRTKVAGQESAAQLRWLAGSLAASTADWKIVVGHHPIH
                     SCGPHGDSAELVRDVLPVLARFHVAVYLNGHEHGLQHLESGGIHFLTSGGGSEATRVS
                     PDARTVWAEATGGFLALAASRETLQVRAVNGLGAVRHEAAIPRPAPVP"
     gene            complement(5065..5535)
                     /locus_tag="PLB01_09080"
     CDS             complement(5065..5535)
                     /locus_tag="PLB01_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQQ77493.1"
                     /translation="MSPTQAPPAKAVTLQPIVFATDFSPASKGALGKAIELARKEGAE
                     LIVAHVYEPPASLSHSGVADDLIEAELEATIRKDVEKQLAPVLAEVRAAGVKVRGEIL
                     AGHTAKELGLLADRNGAAMIVIGTHGRTGIKKLVMGSVASQVIATAPCPVLTVH"
     gene            complement(5646..7337)
                     /locus_tag="PLB01_09085"
     CDS             complement(5646..7337)
                     /locus_tag="PLB01_09085"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQQ77494.1"
                     /translation="MGTKYIFITGGVVSGLGKGIAAASMGALLEARGFKVTLQKFDPY
                     LNVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFTSAVTGKANSITTGKIYQSVIEK
                     ERRGDYLGKTVQVIPHVTDEIKDCIRRVAGDNDVVLVEIGGTVGDIESLPFLEAIRQF
                     RQDVGRGNAINVHVTLVPHIAGSDELKTKPTQHSVKELRSIGIQPDILLCRADRSIPE
                     EMKKKIALFCNVDERAVITAIDVPTIYEVPLAFAREGLDEILLDYLSLPHYDRDLTKW
                     DTIVNRVKNPQDEVNVAFVGKYVQLGDSYKSLNEALHHGGIANNVKVKISFVDSEELE
                     SVGYPETLARADAILVGPGFGRRGIEGKLKAIRFAREMKVPFFGICLGMQCAVIEYAR
                     NVCGLKDANSTEFDTLAPHKVIYMLRDLLGVEALGGTMRLGKYPCDLGEGTLARRAYG
                     EPQIWERHRHRYEVNQEYLPMLRDAGLTFCGMSPDKKFVEMVELSDHPWFLGCQFHPE
                     FKSKPAQPHPLFKDFVRAARAHRLATREGVEKKAAELETQVVAPANLPAPNGLSA"
     gene            complement(7339..8223)
                     /gene="kdsB"
                     /locus_tag="PLB01_09090"
     CDS             complement(7339..8223)
                     /gene="kdsB"
                     /locus_tag="PLB01_09090"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQQ77495.1"
                     /translation="MHRTAASSPLNAAAVIPSRYGAERFPGKPLHPIAGVPMVVRVLE
                     QAQKARRIDLVLVATDDERIARTVEAAGGRAVMTDPALPSGTDRVWAAVKDLDVAVVL
                     NVQGDEPMMDPENVDRVAGFLLDHPEFPLATVALPIDDPTEIANPNNVKVVRADDGRA
                     LYFSRSPLPYRRRPEPGLKTLKHLGLYGYRKDALRRWTGLPPHALERAESLEQLRALA
                     AGMAMAVLDATSDSIGVDTEEDARRVEEILLRMKMGAGAGAAREIGPGPSASSVFTFK
                     EKQRDESRIVNLLATTEV"
     gene            complement(8345..8779)
                     /locus_tag="PLB01_09095"
     CDS             complement(8345..8779)
                     /locus_tag="PLB01_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77496.1"
                     /translation="MIEKLFRSFRAPFGASGREDFTLEEEVALYRHRAEVALEEERYS
                     DALVFLAKILRLNPYDLQARMTVAHTYHYALKEPTKALLTYEKVIAASGYDDSNSYSV
                     AAREGIQELSGAVDTSALPLHDLVEDEGEETPHTAGRANAAG"
     gene            complement(9023..10081)
                     /locus_tag="PLB01_09100"
     CDS             complement(9023..10081)
                     /locus_tag="PLB01_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQQ77497.1"
                     /translation="MSQTSLKDRLLVATLPAEERENGRRLLRALAPIEAKLKEVETVL
                     IDRTRSGIPTIALIGDYLVEGGGKRIRPALLLLGAHMLGYEGDRDVRYGAMIEFIHTA
                     TLVHDDIIDDAETRRGRTSVNRRWGNELTVLFGDFLYMKSMEIALEEGDLRVLRVLSD
                     VTLRMTEGEIIGAERRGLLDLTLEGYLDIVRRKTALLFAGACKIPSYLVPDAFGIGSD
                     TWGERLWDYGLSLGIAFQLQDDLLDYTANEADLGKPVLSDLKEGKLTLPLILCLPQAT
                     RAERKLIETVVKEEGFDSVRPGQILEIVHRLGALDRAHGMAEAHAERAREIAASFPET
                     ASREGFLLAAEYAANRRK"
     gene            complement(10146..10637)
                     /locus_tag="PLB01_09105"
     CDS             complement(10146..10637)
                     /locus_tag="PLB01_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HQQ77498.1"
                     /translation="MADPTFDVVSEVNLPEAQNAVAQAQKEVAQRYDLKGSSAAIEQK
                     ERELTLTADTDFALKAVNDILQTKLVKRGVSLKALEYGKIEPAAKGTVRQVVTIQQGI
                     ATEKAKEIVKAIKDAKLKVQVAIQGEQLRVSGKKKDDLQAVIALLRAADFGIPLQFTN
                     FRG"
     gene            complement(10642..11244)
                     /locus_tag="PLB01_09110"
     CDS             complement(10642..11244)
                     /locus_tag="PLB01_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HQQ77499.1"
                     /translation="MYPALLALLLLAPAGTLSKIRAAYATPGSVACTFVQTYAPAGFA
                     ETAPETGKLVLQAPDRVRFDYDGPEGKVFTFDGTAGRQYVAADRQLVVRKLAPADRER
                     LPIVFLESTEALLARYDAAETPAENGLVEIVLSPKKPGGLRSVSLLAAAASGEVKRLV
                     LVDNAGNRTTFTFMQKIPGAKRPDSDFALSAPEGTKILTE"
     gene            complement(11244..12785)
                     /gene="purH"
                     /locus_tag="PLB01_09115"
     CDS             complement(11244..12785)
                     /gene="purH"
                     /locus_tag="PLB01_09115"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQQ77500.1"
                     /translation="MTARLALLSVSDRTGLVDFARALVAQGYGLLSTGGTAQHLSSAG
                     LAVTNVSDLTGFPEVFGGRVKTLHPKLFGGILFNRSEETHRAEARENGVGPIDLVAVN
                     LYPFEDTVARTGSTFAQAIEKIDVGGPSLLRAAAKNHAHVGVVCDPADYPAVAAELAA
                     HGGALTDETRTRLAAKVFRRTAAYDAAIARWISGQTGEPFPERLSLSFGLASSLRYGE
                     NPHQKASLYADAAAHPAALSRYVFVQGKELSYNNFLDADAALYTSRVVGPNALTIVKH
                     RIPSGAATGAPMAAAFEAAWASDPIAGFGGVVAYTGTLDAAAASALTARFLEVVVVHA
                     VTEDAKPVLAKKPNLRVLTVTPESGPRPRLEARGIDGGLLVQEGDLLPDEEGSWKVVT
                     KRAPTEAERRAMRFANLVLRGVVSNGIVVAGETATYGIGGGRTSRVDACRDAAGKAGE
                     RAKGAVAASDAFFPFPDGLEVLTGAGVTAVIQPGGSVKDPEVIAAADAAGIAMVFTGK
                     RHFRH"
     gene            complement(12782..>13119)
                     /locus_tag="PLB01_09120"
     CDS             complement(12782..>13119)
                     /locus_tag="PLB01_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77501.1"
                     /translation="DTRALAAAIRRHSAFADVAYAAGSIVHAAAMAYAPDAPGTQGPS
                     PFLGFSADPFAAPEALAAAQLPAATSAQRRAAAVTLTARLLAWTWKTAGGDASIVTQY
                     PEAKGPYQP"
BASE COUNT         1862 a         4250 c         4886 g         2121 t
ORIGIN      
        1 gagaggacga ttccggccgt tccgccgttg gcgctgacgg acgtgaacga catcactccc
       61 gtgttgcccg tgccctggat gtcgatcgcc gtcccggtcg tcgtcgtgat cgtccgggcg
      121 gtaacgctga cgcccgtgac ggtcgcgtag ctcgagccgt tgaagttcgt gatcgcccgc
      181 gacgtcgtcg tcgccatgtc gatcccgctc acgttgacga agctcgcgag cgtgagcgtg
      241 cccgacagag tcggcgtgtt cgcggcgctg cccgccaccg tgaggatcgg cgagacggtg
      301 gggacgttga gcgtcgcgcc ggcgccgacg agccgctggc tcgtcttcat cgcgtaggcg
      361 cccgtcgtcg agccggagcc cttcgcgacg tagatgaagt cgccgctgcc ggtcgaggcg
      421 gtcccgaccg ccgccatggt cttgagcggg gtgctcgacc ggccgtcgtt cgtgccggac
      481 gccgtcgcgt tgtcgacgta ccagaccatc ccgctcagcg tgaacgtcag gctcgcggtg
      541 gccgtcgtgg gcgtgggcgt gccggccgtg ttggaggaaa ccgtgtacgt gcacgtcgcc
      601 gacgtggcgg cgacgtccgg cgtgaagatg aagcggccga ccaggtcgaa cgtcgcgatc
      661 gtcccgcccg acgagcagct ggcggcggag acccaggtgg cgccgagcgg ctgcacgccg
      721 ttcagaagag cggtcccgct cgcgacggcc ggcgtcgcgg gggcgcccgc gaccccggtc
      781 acatagacct gagtgttccc gacgcccgtg taggactgcg acggggccgt cggtgccacg
      841 gtcaccgtca cgctcttcga gcccgtgcag ccgcccgcgt ccgtcgccgt gatcgtggcc
      901 gagaacgtgc cggtcgccgt gggcgtgccc gtgacggcgc cggtcgagga gttgatcgcg
      961 acgccgccgg ggagccccgt ggccgaccac gtgatcgtcg aggtcgtccc gctctgcgag
     1021 aaggtcgccg tcgacatcgc cgtgttgtag gcgagggccg ggatcgttcc gctcaccgtg
     1081 atcgtcgggc aggtgaggac gagcgtgtac gtcgccgacg tcccgaagca tccgttcgtg
     1141 tccgtcgcct tgacgacgat cgggaaagag cccgtctcgg tcggcgtgcc ggagaggact
     1201 ccggtcgacg ggttgagcgt cacaccggca ggcagcgtgc tcgcggtcga ccacgtgacc
     1261 gtcccgagga tgccgctcgc cgtgaacgtc tggctgaacg agttgccctc cgtcccggtg
     1321 gtggtcgcgg ggatcgtcac ggtgatcgtc tggcagtcga tgacgagcgt gtaggtcgcg
     1381 cccgtgcccg tgcagccgtt cgcgtccgtt gccttgacga cgatcgggta ggtgcccgcc
     1441 tgcgacgtcg tgcccgagag cgtgccgtcc gccgcgagcg taaggcccgt cggcagcgtg
     1501 ctcgtcgtcg agaacgtcgc cgtgccgctg cctccggact gggagaactg ctggctgaag
     1561 gcgactccgg ccgttccggt cgtcgtcgac ggattcgtta cggcgatcgt cgggcaggtc
     1621 gtcacgaggc tgtagacggt ctgtcccgtg cagccgttcg cgtccgtcgc cgtcaccgtg
     1681 acggggaacg tgcccggctg ggtcggcgtg ccggagagga cgccggccgc gtacgtcatg
     1741 cccggaggca gggcgccgac ctcggcgagc gcgtagggcg cagtgccgcc cgacgccgtg
     1801 aacgtgatgg gaccgtacgg ggagccgacc ggcccggccg cgagcgacgg gggcgcgacg
     1861 gtgatcgtca cgccgttgca cgtgatcgtg acgacgtagt cctggctgcc cgcgcagccg
     1921 cccgcgtcgg tcgccgagat cgtgatcggg aacgagcccg tctgcgtggg cgtgcccgag
     1981 agcgtgtccg tcgagaacgt catgcccgag ggcagggctc ccgccagctg gaaggtgtag
     2041 gggcccgctc cgcccgacgc cgagaagaca gtggccggga agggcgcgtt cgccggggcg
     2101 gccgcgaggg agcccggcga gagcgtgatc ggcgtggacg ggcaggacac gacgaggagg
     2161 tagtcgcgcg tcgccgtgca gccgttcgcg tcggtggccg tgacggtgaa cgggtacgag
     2221 cccgtcaacg ccgtcgtgcc ggaaaggacg ccgcccgacg agagcccgag gcccgcgggg
     2281 agcgtgccgg acgtgacggc gtacgtcacg gcgccgatgc cgcccgactg cgtcagcgtg
     2341 gccgtgtacg cgacgcccga cgtcgcctgc ggaagcgcgg caggcaggat ggaccacgtc
     2401 tggcagccca cgacgagcgt gtacgtggcg ccgattccgg cgcagccgtt cgcgtcggtg
     2461 gccgtgacga cgatcgggaa cgagccggtc tgcgtcggcg tgccggagag gacgccgttc
     2521 gcggcgagcg tgaggccggc cgggagcgtc gacgccgtcg tgaaggtcac ggtcccgacg
     2581 gtgttcgagg ccgtgaacgt ctggctgaac ggggtgttcg cggtcgccgt cgacgtcgcg
     2641 ggattcgaca ccgcgatcgt ctggcagttg atcgtgaggt tgtaggtcgc tccggtcccc
     2701 gtgcagaggt tgccgtccgt gaccttgacc acgatcggga acgtgccttt ctgcgtgggc
     2761 gtgcccgaga gaacgccgtt tgccgcgagc gcgaggcccg ccgggagggt gctcacggtc
     2821 gagaacgtcg ccgagccgat cgcgccggcc tgcgtgaacg tctggctgaa cgccgcgttg
     2881 accgtgcctg tcgtcgtcgc cggattcgtc acggtgatcg tctggcagtt gatgacgagg
     2941 tggtacgtcg cgcccgcgcc cgtacagccg ttcgcgtctg tggccgtgac gacgatgtcg
     3001 aagccgccgg cctgtgtggg cgtgcccgag agaacgccgc tggccgcgag cgtcatcccg
     3061 atcgggaggg cgctccccgt cgagaacgtc accgtcccga cgccgcccga ctgggtgaag
     3121 gtctggctga agggcgtgtt cgccgtcccg gtcgtgacgc cggggttcgt cacggtgatc
     3181 gtctggcagg cgatcacgag cgtgtaagtg ggcccggtgc ccgtgcagcc gttctggtcg
     3241 gtggcggtca cggtgatcgg gaaggtgccg gactgcgtgg gcgtgcccga gagcgtcccg
     3301 ttcgcggcga gcgtcaggcc ggccgggagc ggcgaggacg tcgtgtaggt ctttgcgccg
     3361 actccgcccg aggacgtgaa cgtctggctg aacggggagt tcacggcgcc ggccgccgtg
     3421 gcggggttcg tgacgaggat cgggctcgtg caggcgagga cgaagtagcc ctcgacgtcg
     3481 aggagatagt ccgcgctgcc cgtggacccg ttcgcgagcg cgaccgttcc ggacccgtcc
     3541 gacgcgagcg cgacgaacgc gaagccggcg cgagtcttgc gggcgttgaa gccgaggacc
     3601 gtcgtcggcg gcacgggagc gtcgcccgcg aacagctggg cgtagcccac cgccgcgggg
     3661 ttcacgacgg tgaggttgac gacgaggccg agggcgttcg cggggacgcc gcacgtcccg
     3721 gcgatcgtga agacccgcac ttcgttggcg ccgagcgcgg ggccgccgtt cgggcccgcg
     3781 ggccctcgcg tgtccacggc ccggcaggcc gtgatcggaa cgaacgcctg ggccgcgggt
     3841 ccgacgccgg ccgcgagcgg atgcgccagg gcgagaaggg cggccggaaa gagagcgagc
     3901 ccgcgacgga tcattcgacc tccgctgcgc cgttcacgcg gcgcgctgat tctttgtacg
     3961 agcgctcggg tgagccctct ccggggtgtt gcggcggcga tcgtatctca agtggggaaa
     4021 ccgcgccgcc cggagcggga atgagagcct gacgaattca gggcacgggc gccgggcgcg
     4081 ggatcgcggc ctcgtgccgg accgcgccga gcccgttcac cgccctgacc tggagagtct
     4141 cgcggctcgc cgcgagcgcc aggaacccgc cggtggcctc ggcccagacg gtcctcgcgt
     4201 cgggagaaac gcgcgtggcc tccgacccgc cgccggaggt caggaagtga atgccgccgg
     4261 actcgaggtg ctggagtccg tgctcgtggc cgttcaggta aacggcgacg tgaaagcgcg
     4321 cgaggaccgg caggacgtcg cggacgagct cggccgagtc cccgtgcggc ccgcacgaat
     4381 ggatcgggtg gtgcccgacg acgatcttcc agtccgcggt cgaggccgcg aggctgccgg
     4441 cgagccaccg gagttgcgcc gccgactcct gcccggcgac cttcgtgcgg gagaggaacg
     4501 aacgataggt cgcgaggaag ggcgtcgtgt ccatgacgaa gaactcgacg cgggttccgt
     4561 ctccggcggt ctcctgcacg gtgaacgagc gcgccggcat gcgccagcgg gggctcgact
     4621 tcgagtattc gacctgtgcc tccacgtttc cccggtagtc gtggttgccg agcgcgacga
     4681 tccagggcag ccggaggaga ctcggcgccg cgtagacgtc ttcgaacgag cccttccagg
     4741 cccggtccgt cacggacgaa accccgtctt cgtagaagtt gtcgcctgtc gagacgacga
     4801 atcggatcgg gcgctttccg gcccattccg ccatcgcgcc ggcgacctgg cgctgcacac
     4861 ggttgccgcc gcgcccccag tcgccgagga cgaggaactc gagcgatccg gccggcggct
     4921 gcggcagcac ggccgcggcc gtcggcgtcg tgcgcgccgg cggcgcggga gacggcccgc
     4981 acgcggcgac ggcggcggcg agcagggccg ccccggcgag acgtcgaagc acgcttcgat
     5041 gctagcaggg gcggtttcgc gggctcagtg gacggtcagc accgggcacg gcgccgtcgc
     5101 gatgacctgg ctcgcgacgc tgcccatgac gagcttcttg atgcccgtcc tgccgtgcgt
     5161 tccgatgacg atcatcgccg ctccgttgcg gtccgcgagc aggccgagct ccttggccgt
     5221 atggccggcg aggatctcgc cgcgcacctt gacgccggcc gcacgaacct ccgcgagcac
     5281 gggcgcgagc tgtttctcga cgtccttgcg aatcgtcgcc tcgagctcgg cctcgatgag
     5341 gtcgtccgcg acgccggaat ggctcaggct cgcgggcggc tcgtagacgt gcgcgacgat
     5401 cagctcggcg ccttccttgc gggccagctc gatcgccttc ccgagggcgc ccttggacgc
     5461 cggcgagaag tccgtcgcga acacgatcgg ctgcagggtc accgccttcg cgggcggggc
     5521 ttgggtcgga ctcatttgcg gcgttgccat cgcgccacct cccatgccct catcaacgcg
     5581 atagggcgcg gagcacggcc cggccatgag gcatttgaat caggatccgt gtcttcgcgg
     5641 gagattcagg ccgagaggcc gttcggggcg gggaggttcg ccggcgcaac gacctgcgtt
     5701 tccagctcgg ccgccttctt ttccacaccc tcgcgcgtcg cgagccggtg ggcgcgcgcg
     5761 gcgcggacga agtccttgaa gagcgggtga ggctgggccg gcttgctctt gaactccggg
     5821 tgaaactggc acccgaggaa ccacgggtgg tccgagagct cgaccatctc gacgaacttc
     5881 ttgtccggcg acattccgca gaacgtcagg ccggcgtcgc gcagcatcgg caggtattcc
     5941 tggttgactt cgtagcggtg gcggtggcgc tcccagatct gcggctcgcc gtaggcgcgc
     6001 cgggcgagcg tcccttcgcc gaggtcgcac gggtacttgc cgaggcgcat cgtcccgccc
     6061 agcgcttcga cgccgaggag gtcgcgcagc atgtagatga ccttgtgggg agcgagcgtg
     6121 tcgaactcgg tcgagttcgc gtccttcagc ccgcagacgt tccgcgcgta ctcgatgacg
     6181 gcgcactgca tcccgaggca gatcccgaag aacgggacct tcatctcgcg cgcgaagcgg
     6241 atcgccttga gcttgccctc gatcccgcgg cggccgaagc cgggcccgac gaggatcgcg
     6301 tccgcgcggg cgagcgtctc gggatagccc acgctctcga gctcctcgga atccacgaac
     6361 gagatcttca ccttcacgtt gttcgcgatg ccgccgtggt ggagcgcctc gttcagcgac
     6421 ttgtagctgt cgccgagctg cacgtacttg ccgacgaacg cgacgttgac ctcgtcctgg
     6481 gggttcttga cgcggttgac gatcgtgtcc catttcgtga ggtcgcggtc gtagtggggg
     6541 agggagaggt agtcgaggag gatctcgtcg agcccctcgc gcgcgaacgc gagcgggacc
     6601 tcgtagatcg tcggaacgtc gatcgccgtg atgacggcgc gctcgtccac gttgcagaag
     6661 agcgcgatct tcttcttcat ctcctcgggg atcgagcggt ccgcgcggca gaggaggatg
     6721 tcgggctgga tgccgatcga gcgcagctcc ttgacggagt gctgcgtggg cttcgtcttc
     6781 agctcgtcgg agcccgcgat gtgcgggacc agggttacgt ggacgttgat cgcgttgccg
     6841 cgcccgacgt cctgccggaa ctggcggatc gcctcgagga acgggaggga ctcgatgtcg
     6901 ccgacggtcc ctccgatctc gacgaggacg acgtcgttgt cgcccgcgac gcggcggatg
     6961 cagtccttga tctcgtccgt gacgtgcggg atgacctgga ccgtcttgcc gaggtaatcg
     7021 ccgcgccgct ccttctcgat gacggactgg tagatcttgc cggtcgtgat gctgttcgcc
     7081 ttgcccgtga cggcggaggt gaagcgctcg tagtggccga ggtcgaggtc cgtctcggcg
     7141 ccgtcgtccg tgacgtagac ctcgccgtgc tggaacggag acatcgtgcc ggggtcgacg
     7201 ttcaggtacg ggtcgaactt ctgaagcgtg accttgaagc cccgggcctc gaggagcgcg
     7261 cccatcgagg ccgccgcgat gcccttgccg aggccggaaa ccacgccacc cgtgatgaag
     7321 atgtatttgg ttcccacgct agacctccgt cgtggccaga aggttgacga tccggctttc
     7381 gtcacgctgc ttttctttga aggtgaatac ggaggaggcg gatgggccag gcccgatctc
     7441 gcgagcggcg cccgcgccag cgcccatttt cattctcaga agtatctctt ctactcttct
     7501 cgcgtcttcc tcggtatcga caccgatcga gtcgctcgtc gcgtcgagga ccgccatcgc
     7561 catccccgcc gcgagcgcgc ggagctgctc gaggctctcg gcccgctcga gcgcgtgggg
     7621 cggcaggccg gtccagcgcc ggagcgcgtc cttgcggtac ccgtagagcc cgaggtgctt
     7681 gagggttttc aggccgggct cgggccgccg gcggtacggg aggggcgagc gggagaagta
     7741 gagcgcgcgg ccgtcgtcgg cgcgcacgac cttcacgttg ttcgggttcg cgatctcggt
     7801 ggggtcgtcg atcggaagcg cgaccgtcgc gagcgggaac tcggggtggt cgaggaggaa
     7861 gcccgcaacg cggtcgacgt tttcgggatc catcatcggc tcgtcgccct gcacgttcag
     7921 cacgacggcg acgtccaggt ccttcacggc cgcccagacg cgatccgttc ccgaggggag
     7981 cgcgggatcg gtcatgaccg cccggccgcc cgccgcctcc acggtccgtg caatccgctc
     8041 gtcgtccgtc gcgacgagga cgaggtctat gcggcgggcc ttctgcgcct gttcgagcac
     8101 gcggacgacc atcgggacgc cggcgatcgg gtgcaggggt ttgcctggga aacgctccgc
     8161 cccgtagcgg gaggggatga cggcggcggc gttgaggggg gaggaggccg ccgtccggtg
     8221 cacgttcgat cctaactcgg cgtgcgcgag cccgtcaacg aactccagcc ccgaaacggg
     8281 aagtcgtcac cggcccggag ggtcgatgac gacgcgaggg agaaaaacgg gattcggggg
     8341 gcttctatcc ggccgcgttg gctcggccgg cggtgtgcgg ggtttcctcg ccctcgtcct
     8401 cgacgaggtc gtgaaggggg agcgccgacg tgtcgacggc gccggacagc tcctggattc
     8461 cctcgcgagc cgcgacggag taggagttgg aatcgtcgta accggaggcc gcgatgacct
     8521 tctcgtacgt gaggagtgcc ttcgtgggct ccttcagtgc gtagtggtac gtgtgggcca
     8581 cggtcatccg ggcctgcaga tcgtagggat tgagccggag gatcttcgcg aggaagacga
     8641 gcgcgtccga gtagcgctcc tcctcgaggg cgacttccgc ccggtggcgg tagagcgcca
     8701 cttcctcttc gagagtgaag tcctcgcgtc ccgaagcgcc gaacggcgcg cggaacgaac
     8761 ggaacagctt ttcgatcatg agttctcctg atcgtccccg aagctgtggg gtgctttgct
     8821 catacccacg ataggttgtg gttaaggtac gactgtcgag aaccgaaatc aagagaagaa
     8881 ccagaaaaaa gaaggaaatt cttagaaggt gccttcttta tttcggctac ggcgcgcaca
     8941 tgaggcgaag acaccgcatg cattaggcag atttcgcatc tgccctatta tcattcgaag
     9001 aaactatctt atttattttt aattacttac gtcggttcgc ggcgtattcc gcggcgagga
     9061 ggaagccctc gcgcgaggcc gtctcgggga aagaagccgc gatctcgcgg gcccgttcgg
     9121 cgtgggcttc ggccatcccg tgcgcgcggt cgagcgcgcc gaggcggtgg acgatttcga
     9181 ggatctgccc gggccgcacg gagtcgaagc cctcttcctt cacgaccgtc tcgatgagct
     9241 tccgttcggc gcgcgtcgcc tgcggaagac agaggatgag cggaagcgtg agcttccctt
     9301 ccttgaggtc cgacaggacc ggcttgccga ggtcggcctc gttcgccgtg taatcgagca
     9361 ggtcgtcctg cagctggaac gcgatcccga gcgagaggcc gtagtcccag aggcgctccc
     9421 cccacgtgtc gctgccgatt ccgaaggcgt ccgggacgag gtacgacggg atcttgcagg
     9481 cgcccgcgaa gagcagggcg gtcttccgcc gcacgatgtc gaggtagccc tcgagcgtga
     9541 ggtcgagcag cccgcgccgc tcggcgccga tgatctcgcc ctccgtcatg cgcagcgtga
     9601 cgtccgagag gaccctgagg acgcgcagat cgccctcttc gagcgcgatc tccatggatt
     9661 tcatgtagag gaagtcgccg aacaggacgg tcagctcgtt gccccagcgg cggttcacgc
     9721 tggtccgccc gcggcgggtc tcggcgtcgt cgatgatgtc gtcgtggacg agcgtcgccg
     9781 tgtgaatgaa ctcgatcatg gcgccgtagc gcacgtcgcg gtcgccttcg tacccgagca
     9841 tgtgcgcgcc caggagaagc agcgccggcc ggatgcgctt gccgccgccc tcgacgaggt
     9901 aatcgccgat cagggcgatc gtcgggatcc cggagcgcgt ccggtcgatg aggacggtct
     9961 cgacctcctt gagcttcgcc tcgatggggg cgagggcccg gaggaggcgg cggccgttct
    10021 cgcgctcctc ggccgggagc gtcgcgacga gaaggcggtc cttgagggag gtctggctca
    10081 tacgcgcggg cgcatcttac cgcccctttc gaccggttta cgcccggcgg agggagcagg
    10141 atgctctagc cgcggaaatt cgtgaactgg agcgggatgc cgaagtccgc ggcgcggaga
    10201 agggcgatca cggcctgcag gtcgtccttc ttcttgcccg aaacccgcag ctgctcgccc
    10261 tggatggcga cctggacctt cagctttgcg tccttgatcg ccttgacgat ctccttggcc
    10321 ttctcggtcg cgatgccctg ctggatcgtg acgacctggc gcacggtgcc cttcgcggcc
    10381 ggctcgatct ttccgtactc gagggccttc agggagacgc cgcgcttgac gagcttcgtc
    10441 tggaggatgt cgttcacggc cttgagggcg aagtcggtgt cggcggtgag cgtgagctcg
    10501 cgttccttct gctcgatggc cgcggacgag cccttgaggt cgtagcgctg ggcgacctct
    10561 ttctgggcct gcgcgacggc gttctgggcc tcggggaggt tcacttcgga gacgacgtcg
    10621 aacgtggggt cggccatggg atcactccgt gaggatcttc gttccctccg gcgcggagag
    10681 ggcgaagtcc gagtcggggc gcttggcccc ggggattttc tgcatgaacg tgaacgtcgt
    10741 tcggttgccg gcgttgtcca cgaggacgag gcgcttgacc tccccggacg cggcagccgc
    10801 gagaagggac acggacctca ggccgccggg cttcttcggg ctgagcacga tctcgacgag
    10861 gccgttttcc gcgggggtct cggcggcgtc gtagcgcgcg aggagcgcct cggtggattc
    10921 gaggaacacg atggggagcc gctcgcggtc ggccggagcg agcttccgga cgacgagctg
    10981 cctgtcggcc gcgacgtact ggcgcccggc cgtcccgtcg aacgtgaaga ccttgccctc
    11041 cggaccgtcg tagtcgaagc gcacgcggtc gggcgcctgc aggacgagct ttccggtttc
    11101 gggcgcggtc tcggcgaagc cggcgggggc gtacgtctgg acgaacgtgc aggccacgga
    11161 acctggcgtc gcgtacgcgg cgcggatctt cgagagggtg ccggccggcg cgaggagaag
    11221 cagggcgagc agcgcggggt acatcaatgc ctgaagtgtc tcttccccgt gaacaccatc
    11281 gcgatgccgg ccgcgtccgc cgcggcgatg acctcggggt ctttcacgga gccgccgggc
    11341 tggatgaccg ccgtgacgcc cgcgcccgtc aggacctcga gcccgtcggg gaacgggaag
    11401 aacgcgtccg acgccgccac ggcgcccttc gcgcgctcgc cggccttgcc ggcggcgtcg
    11461 cggcaggcgt cgacgcggct cgtgcggccg cctccgattc cgtaggtcgc cgtctcgccc
    11521 gcgacgacga tgccgttcga cacgacgccg cgcaggacga ggttcgcgaa gcgcatcgcg
    11581 cggcgttcgg cctcggtcgg ggcgcgcttc gtaacgacct tccaggaccc ctcctcgtcc
    11641 gggaggaggt cgccctcctg cacgagcagg ccgccgtcga tgccccgcgc ctcgaggcgc
    11701 gggcgcgggc cggactccgg cgtgaccgtg aggacgcgca ggttcggctt cttcgcgagg
    11761 acgggcttcg cgtcctcggt gacggcgtgc acgacgacga cctcgaggaa gcgcgcggtc
    11821 aacgccgacg cggcggccgc gtcgagcgtc ccggtgtacg cgacgacgcc gccgaagccc
    11881 gcgatcgggt ccgacgccca tgcggcctcg aaagccgcgg ccatgggcgc gccggtcgcc
    11941 gcgccggacg ggatccggtg cttgacgatc gtgagcgcgt tcggcccgac cacgcgcgac
    12001 gtgtagaggg ccgcgtccgc gtcgaggaag ttgttgtacg agagctcctt gccctggacg
    12061 aagacgtagc gggagagcgc cgccgggtgg gccgcggcgt cggcgtagag ggacgccttc
    12121 tgatgcgggt tctcgccgta gcgcagggag gacgcgagcc cgaaggagag ggacaggcgc
    12181 tcggggaagg gctcgcccgt ctgcccggag atccaccggg cgatcgcggc gtcgtacgcg
    12241 gcggtgcggc ggaagacctt cgcggcgagc ctcgtccgcg tctcgtccgt cagggcgccg
    12301 ccgtgcgcgg ccagctccgc ggcgacggcg gggtagtccg ccgggtcgca gacgacgccg
    12361 acgtgcgcgt ggttcttcgc ggccgcgcgg aggagcgacg gcccgccgac gtcgatcttc
    12421 tcgatcgcct gcgcgaacgt cgagccggtc ctcgcgacgg tgtcctcgaa cgggtagagg
    12481 ttcacggcga cgaggtcgat cgggccgacg ccgttctcgc gggcctcggc gcggtgcgtc
    12541 tcctcggagc ggttgaagag gatgccgccg aagagcttcg ggtggagcgt cttgacgcgc
    12601 ccgccgaaga cctcgggaaa ccccgtgagg tccgagacgt tcgtgacggc gaggccggcg
    12661 gaggagaggt gctgcgccgt gccgccggtc gagaggaggc cgtacccttg cgcgacgagg
    12721 gcgcgggcga agtcgacgag tcccgtgcgg tcggagacgg agaggagcgc gaggcgggcg
    12781 gtcacggctg gtagggtcct ttcgcttcgg ggtactgcgt cacgatcgac gcgtcgcctc
    12841 cggccgtctt ccacgtccac gcgaggaggc gggccgtcag cgtgaccgcg gcggcgcggc
    12901 gctgggcgga ggtcgcggca gggagctgcg cggcggcgag cgcctcgggc gcggcgaagg
    12961 ggtcggcgga gaaaccgaga aacggcgaag ggccctgcgt cccgggggcg tcgggcgcat
    13021 aggccatcgc ggccgcgtgc acgatcgagc cggccgcata ggcgacgtcc gcgaacgccg
    13081 agtggcgcct gatcgcggcc gcgagggcgc gggtgtccc
//
LOCUS       DAOMFY010000089        13080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1462_length_13080_cov_8.755317, whole genome shotgun sequence.
ACCESSION   DAOMFY010000089 DAOMFY010000000
VERSION     DAOMFY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1462_length_13080_cov_8.755317"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLB01_09125"
     CDS             complement(<1..184)
                     /locus_tag="PLB01_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77502.1"
                     /translation="MKPRRHDVAREHRVEEARGRRDPERVGREEQALGVVRDLRSVPR
                     SVLEAGLRCLQGKGIVN"
     gene            167..1618
                     /locus_tag="PLB01_09130"
     CDS             167..1618
                     /locus_tag="PLB01_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HQQ77503.1"
                     /translation="MAPGLHAHDLAGDARGVLNLPPESVPSAKDLNGGDAPSPATFAT
                     EEGRRFLAPRVLLPLALADALAPRLSEGSPLSRVLEELELPLVPVLARMEKAGVAVDR
                     GVLAGLSKEFDGRLAALEAKIHEAAGEPFNIGSPLQLGRVLFEKLGYPAVKKTAKTKS
                     YATGSEVLEELAALSSGPVPSLVLEWREISKLKGTYVDALPAYIASDGRVHTRYDQAV
                     AATGRLSSNAPNLQNIPIRTEAGRAIRRAFVAPEGTKLVAADYSQIELRILAHLSGDE
                     ALIDVFRRGEDIHRATAAKIFGLDLAAVTPDQRRSAKTINFGVLYGMGPFALAAQLGV
                     PRAEAKEFIAAYFERFPKIRACLDAILEKARATGGTETILGRVRPIPGLHDRNHNVRA
                     NAERMAMNAPFQGSAADLVKKAMLALDAALEKELPGVRLLLQVHDELVLEAPAALAEK
                     AAALARRTMEGAASLAVPLTVDVGIGLNWAEAK"
     gene            1646..2527
                     /locus_tag="PLB01_09135"
     CDS             1646..2527
                     /locus_tag="PLB01_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77504.1"
                     /translation="MRRLYGFLGAAALAASGVLSAQTAALKPVEALPDPVRPALAEVP
                     YAHPLFLALEEPPKVLERPNPVDLETRVPLVVIARVLETGKVSELALVEPPLKALATP
                     LPALAPRWRYAIPRKGGQPAVTWATQILELNVSLEKPVFSAFQFEPLKKDDPLARVFR
                     ENSGDDWMARYPAAIDPKDAEAVSVEELDAPPAAEKTPWSFDATRQRGRITALVEISA
                     QGTVSRVLPTGDAEPLLVRWVRESAAKWKLSPGKAGGKAVASWMSLDATLDYTIDSAK
                     KKGERSVKKNLRALPPP"
     gene            complement(2570..4486)
                     /gene="selB"
                     /locus_tag="PLB01_09140"
     CDS             complement(2570..4486)
                     /gene="selB"
                     /locus_tag="PLB01_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HQQ77505.1"
                     /translation="MRRVLVGTAGHIDHGKSALVNALTGTDPDRLPEEKARGITIDLG
                     FAHAAWDDTTFSFVDVPGHEKFVRTMVAGAQGVDLVLLAVASDDSVMPQTREHLDILR
                     LLRVSTGVIARTKSDLVDAETGALVEEEIRALVKGSFLADAPIVACSAVTGDGLEALK
                     KALLAAARRVVREDRSRKVPRLFLDRAFTMKGFGPVVTGTLDAGRIAVEDALTLLPDG
                     VDVRVRRVEVHGEERREAFAGERTSLNLAGVDRGDLARGQALVPKGALDAASVLTTEV
                     TILPGAAAALPDGVRVRVHLGTADVAAKLSRAVPGPDDAAGRAFQPGETAFAQLFLES
                     PVAARPGDRFILRRPSPAETLGGGRVLDTGRARMKGRGGASAAVFPVLASGDEKALAD
                     LFLLEAGPAGLTAARLGARLGIVSAAAANLLEALSSEGRALRITPALFAHASVASDLA
                     GRAAALFGERRKAGSAAVALGKGEFLARLAKGISPAAADGWLATLAAGKSIALEGDRV
                     VPPGAKAADLSGEAVSFAAKLEELYRKSGFEPPKTFDAAKVLGTKPQVVEGLASHLVK
                     SGVLVRLSPELVVHREVASAAEKKLETVRGQTLAVAGFRDLLGLTRKTLIPLLEYFDS
                     RKLTRRVGDLRKVE"
     gene            complement(4530..6143)
                     /locus_tag="PLB01_09145"
     CDS             complement(4530..6143)
                     /locus_tag="PLB01_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77506.1"
                     /translation="MFRSDDDGRSWKPARSGLPAGSVGLFPEVWGLAVAPQDGTVFVA
                     FGRFGFAGTPAVFRSTDHGETWQPAGAALTQPAGPVAVDSTDSRTVYAADSGGRLFRS
                     TNSGTSWAPVLEGPAALIRGGSAAREVFTVVVSAANVSLLRSIDAGASWQSTRIGNNV
                     PSDLTSTHQGALVADGIEGVAMYDVATGALKSSALAASLHSGITVTTAGSVITSAVGD
                     GLFRSTDGGTNWTRYEGTLAPAFQLASPVVLAGRHIGTPVFAGASAPLGLSLSDDDGS
                     SWRPANIGMAHGDQTAPVISVAFSPSAPCTAYAGGEAAGVFLSKDCGNSWSATAAPFS
                     GLSLPSVSALAVDPLDSRTVYAGLSTFPFGIVKSVDGGETWSEANAGLDGGGRPRIQA
                     ILADARSSGTLFAGTSNGVFKTVDGGISWERLALGLPETWISSLAGRTEAAVDLFAAT
                     GAPAGQRSRLGRIYRLSLETGYWIDVSGDLPNVPVLSLAIDGRTEPQLWVGTEGAGLY
                     TSTIQALSRHHQQAWPSPGVTVVHPRTKP"
     gene            complement(6563..8065)
                     /locus_tag="PLB01_09150"
     CDS             complement(6563..8065)
                     /locus_tag="PLB01_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77507.1"
                     /translation="MLTRVESLEGATSKDVLVPLSCNTCSELSIEVSVREKTALVASR
                     TFRFLAAGGTMTFVAEGEFEAMAARASEQELVSAGDALAARAMARLRARGRVGVSSAV
                     ARSTASFPARSLPAPSSQSQQAWDRLTSSVRDSGRRPQTNCGDYKFANGENYYHSLSG
                     TAFYNYGGGAYSLRPGYPARIYTQIELGDECGNTIMFTRITETTISDYGSYAVGVYSA
                     EPPGGDNPVIEFHATAPDVGYGNALPLNTVEQVLNPAGPGYSGFYWSGGSPVFNPVFS
                     DAASGSGFSPYSSFFQWNNKIKELRDYWSGQGLASYTAFKAVYDPNTVFSVPYFFGGK
                     VVFEPPFKWLDDWTMTHEFGHNLQFLVQGEQIAYAGGTNLSCTPQNASGSFTEAFADW
                     HATFLSGNPGGIPSSNGDLYSSCASGGSSVSMNIFAFLWDVFDSVNATGPDGNPPVDS
                     VQYSSAYIFGNWGGHRYDTFDEFYNDFEGRGLWSGQNVAAIKQVNRVASP"
     gene            complement(8847..10502)
                     /locus_tag="PLB01_09155"
     CDS             complement(8847..10502)
                     /locus_tag="PLB01_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQQ77508.1"
                     /translation="MPRYRLAGVRARTPKIVNLATVARLLGIPREGLSDFALVRRSLD
                     ARQKPVLYWEIAVEFTSATDLDLTPEAPLRLSPAEPPPAPAPLLPELDTKAKVAIVGT
                     GPAGLFAALALVDAGVPVTILERGDPAEDRYRKVMKFWTSGVFDPESNVQFGEGGAGT
                     FSDGKLTCGKRQDKVKVVLETFHRFGAPDSILVDAKPHIGTDRLVVVLRNIRKHLLEH
                     GATLRYRAKVTDLRTRPDGSLAGLTLESGEDVPADVAIFALGHSARDTMDVLNARGVA
                     MTRKHFAMGVRIEHPQDAVDLIQYAQVSTECGVQPADYKQAHVASNGRGIYTFCMCPG
                     GEVIACSSEPGGVVTNGMSRYKRSSGYANAGIVVQTKDVDFDAMPGPELLRGRELQRR
                     VEAAAFAAAGGTYAAPALRVTDFLRSRERPISPRPLPKTTYGPWVTPVSFDGILPEPY
                     LEALAEGLKAFDRKMPGFVSEEAVLFAPESRTSSPVRIERDGWCESTSVPGLYPAGEG
                     AGFAGGIVSAALDGLRVARHVRARLAGERLEPEKSTIFKGPEY"
     gene            10536..11099
                     /locus_tag="PLB01_09160"
     CDS             10536..11099
                     /locus_tag="PLB01_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HQQ77509.1"
                     /translation="MHGPREIETKFRVRDRGALEERLAGLGARPGPREDETNVLYDEP
                     GGRLRAEGCALRLRTVNERGLLTFKGPPEVSGGIKSRVEWESEVDSPRAVAGILESLG
                     YRPSFRYHKLRTTWNFSDPARPVVVVDEIPLGLFAEIEGPDPAVRALAAELGVAEADF
                     IAESYVALWLAARAKDPSLPEDMVFGR"
     gene            11096..11839
                     /locus_tag="PLB01_09165"
     CDS             11096..11839
                     /locus_tag="PLB01_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQQ77510.1"
                     /translation="MKALVLAAGFGTRFRPATLSTPKPLLPLRGRPILFHVFDHLLGE
                     GADRFVVNAHHLARELHAAVGDSYAGVPVDWSFEERILGTAGGIRCAFERGRLGDHFL
                     VANGDVATTLPLGRLLAARHEPGVVSALAVLPNEDPERDTPLWADAAGRLVAVGGPRP
                     GSATGPWLFTGLQAATAALALRIPEGFAELAKDVLVPSAAAGDGAFALVPYTKADGLW
                     FDLGTPERLAAAEAALAAASRPEPDATRG"
     gene            complement(11836..12381)
                     /locus_tag="PLB01_09170"
     CDS             complement(11836..12381)
                     /locus_tag="PLB01_09170"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HQQ77511.1"
                     /translation="MRKAALLLLALCFLSGNADAIPVVWQLNGVTFADGGTASGSFVY
                     DSSTNTYSSVNITTTPGTVLATGATFLFVSPGVSPTSSGFLAGASNVPNLTGTRAFAL
                     FFASPLSSNGGLVNLSSGLEASCANATCGSPAAPSRSTNAGSVNGTATSPIPTLSAWA
                     LLATGLLLAAAGFAALRRGVF"
     gene            complement(12446..>13080)
                     /locus_tag="PLB01_09175"
     CDS             complement(12446..>13080)
                     /locus_tag="PLB01_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77512.1"
                     /translation="PSPLLTRTKGGTMRASERGVSLAELAVVLAILGALFLISIPALS
                     EILAEESLATAAREVAAILHAARARAVFQGVDVGVKWVANAGDLVLTVYQDGNANGVT
                     TADIRKGVDRLVAGPYWLGSRYPGVAFSFVPGMDGTDPGGAPIGNLSDPVRFGRSNIC
                     TFSPVGHASPGTVYLSNRRNRQAAVRVTPINAKIQIWTWHGKKLKWIARW"
BASE COUNT         2149 a         4481 c         4503 g         1947 t
ORIGIN      
        1 aattgacgat gcccttgccc tgcagacagc gcaggcccgc ctcgagcacc gacctcggca
       61 ccgaccgtag gtcgcgcacg acgccaagcg cctgttcctc gcggccgacg cgctcgggat
      121 cgcgccgccc gcgcgcttcc tcgacgcgat gctcgcgagc tacgtcgtgg cgccggggct
      181 tcacgcgcac gatctcgccg gcgacgcccg cggcgtcctg aacctcccgc cggagtccgt
      241 gccgtcggcg aaggacctga acggcggcga cgcgccgtcg cctgcgacgt tcgcgaccga
      301 ggagggccgc cgcttcctcg cgccccgcgt cctcctcccg ctcgccctcg cggacgcgct
      361 cgccccgcgc ctgtcggagg gctcgccgct ctcgcgggtg ctcgaggagc tcgagcttcc
      421 gctcgttccc gtcctcgcgc ggatggaaaa ggcgggcgtc gccgtcgacc ggggcgtcct
      481 cgcgggcctc tcgaaggagt tcgacggccg gctcgcggcg ctcgaggcaa agatccacga
      541 ggcggcgggc gagcccttca acatcggatc gcctcttcag ctcggcaggg tgctcttcga
      601 gaagctcggc tacccggcgg tcaagaagac ggcgaagacg aagtcgtacg cgacgggctc
      661 ggaggtcctc gaggagctcg cggcgctctc gtcggggccg gtgccctccc tcgtcctcga
      721 atggcgggag atctccaagc tcaagggcac gtacgtcgac gcgctgccgg cgtacatcgc
      781 ttccgacgga cgtgtgcaca ctcggtacga ccaggccgtc gcggcgaccg gccggctctc
      841 ctcgaacgcg ccgaacctcc agaacatccc gatccgcacg gaggcgggcc gtgcgatccg
      901 gcgcgcgttc gtcgcgccgg aagggacgaa gctcgtcgcg gcggactact cgcagatcga
      961 gctccggatc ctcgcccatc tctcgggcga cgaggcgctg atcgacgtct tccggcgcgg
     1021 cgaggacatc caccgcgcca cggcggcgaa gatcttcgga ctcgacctcg ccgccgtcac
     1081 cccggaccag cgccggagcg ccaagacgat caacttcggc gtcctctacg gaatgggccc
     1141 gttcgccctc gccgcgcagc tcggcgttcc gcgcgccgag gcgaaggagt tcatcgcggc
     1201 ttacttcgag cggttcccga agatccgggc ctgtctcgac gcgatcctcg agaaggcgcg
     1261 cgcgacgggc ggcaccgaga cgatcctggg gcgcgtgcgc ccgattcccg gcctccacga
     1321 ccgcaaccac aacgtccgcg cgaacgcgga gcgcatggcg atgaacgcgc cgttccaggg
     1381 ctcggccgcc gacctcgtca agaaggcgat gctcgccctc gacgccgcgc tcgagaagga
     1441 gctgccgggc gtccgcctgc tcctccaggt gcacgacgag ctcgtcctcg aggcgccggc
     1501 ggccctcgcg gagaaggccg cggccctggc gcggcgcacg atggagggcg ccgcgagcct
     1561 cgccgtcccc ctcacggtgg acgtcgggat cggcctcaac tgggccgagg cgaagtaaag
     1621 aggggagagg ccctagaatc gcgccatgcg gaggctctac ggtttcctcg gcgcggccgc
     1681 tctggccgcg agcggcgtcc tctccgcgca gacggcggcg ctcaagcccg tcgaggcgct
     1741 gcccgatccc gtgcgcccgg ccctcgccga ggtcccgtac gcgcatccgc tcttcctcgc
     1801 cctggaggag ccgccgaagg tcctcgagcg cccgaacccc gtcgacctcg agacgcgcgt
     1861 cccgctcgtc gtcatcgcgc gcgtcctcga gaccggcaag gtgagcgaac tcgccctcgt
     1921 cgagccgccg ctcaaggcgc tcgcgacccc gctcccggcg ctcgccccgc gctggcgata
     1981 cgcgattccg aggaagggcg gccagcccgc cgtcacgtgg gccacgcaga tcctcgagct
     2041 gaacgtctcg ctcgagaagc ccgtcttcag cgccttccag ttcgagccgc tcaagaagga
     2101 cgacccgctc gcccgggtat tccgcgagaa ctccggcgac gactggatgg cccgctaccc
     2161 ggccgcgatc gacccgaagg acgccgaggc cgtctccgtc gaggagctgg acgctccccc
     2221 ggcggccgag aagacgccgt ggagcttcga cgccacgcgc cagcgcggcc ggatcacggc
     2281 cctcgtcgag atctcggcgc agggcaccgt ctcgcgcgtt ctcccgacgg gcgacgccga
     2341 gccgctcctc gtgcgctggg tgcgcgagag cgccgcgaag tggaagctct ccccgggcaa
     2401 ggcgggcggc aaggcggtcg cgtcgtggat gtcgctcgac gcgacgctcg actacacgat
     2461 cgactcggcc aagaagaagg gcgagcggtc ggtcaagaag aacctgcggg cccttccacc
     2521 gccatgacct tggggaaccc gaccgccggg ttccccaaac ccctccgcgc tactccacct
     2581 tcctgagatc cccgacccgg cgcgtgagct tccgcgagtc gaagtactcg agcagcggga
     2641 tcagcgtctt gcgtgtcagg ccgaggaggt cgcggaagcc cgcgaccgcg agtgtctgtc
     2701 cccggacggt ctccagcttc ttctcggcgg cggaggcgac ttcccggtgt acgacgagct
     2761 cgggcgaaag gcggacgagg acgcccgact tcacgaggtg cgaggcgagg ccctcgacga
     2821 cctgcggctt cgtcccgagg accttcgcgg cgtcgaaggt cttcggcggc tcgaagccgg
     2881 acttccggta gagctcctcg agcttcgcgg cgaacgacac ggcctcgccc gagaggtccg
     2941 cggccttcgc gccgggcggg acgacgcggt cgccctcgag cgcgatcgac tttccggccg
     3001 cgagcgtcgc gagccatccg tcggcggccg cgggggagat ccccttcgcg aggcgggcga
     3061 ggaactcgcc cttcccgagc gcgacggcgg ccgagcccgc ctttcgccgc tcgccgaaga
     3121 gggcggccgc gcggcccgcg aggtccgacg cgaccgaggc gtgtgcgaag agcgcgggag
     3181 tgatccggag cgcacggccc tcggacgaga gagcctcgag gaggttcgcg gcggcggccg
     3241 acacgatccc gagccgcgcg cccagccgcg cggccgtcag gccggcggga ccggcctcga
     3301 gcaggaagag gtccgcgagc gccttctcgt cgcccgaggc gagaaccggg aagacggccg
     3361 cgctcgcgcc gccgcgcccc ttcatccgcg cgcgcccggt gtcgaggacg cgcccgcctc
     3421 cgagcgtctc cgcgggagat ggccggcgca ggatgaagcg gtcgcccggg cgggccgcga
     3481 cgggcgactc gagaaacagc tgggcgaacg ccgtctctcc cggctggaac gcccggccgg
     3541 ccgcgtcgtc cggccccgga accgcccgcg agagcttcgc cgcgacgtcg gcggtgccga
     3601 ggtgaacgcg gacgcgcacc ccgtcgggca gggcggccgc ggccccgggg aggatcgtca
     3661 cctccgtcgt gagaacggac gccgcgtcca gcgcgccctt cggaacgagc gcctgtccgc
     3721 gggcgaggtc cccgcgatcg acgcccgcga ggttcaggct cgtgcgctcg cccgcgaagg
     3781 cctcgcgccg ctcctcgccg tggacttcca cgcgccgcac gcgcacgtcg acgccgtccg
     3841 gcaggagcgt gagcgcgtcc tcgacggcga tgcgccccgc gtcgagcgtc cccgtcacga
     3901 cgggcccgaa gcccttcatc gtgaacgcgc ggtcgaggaa gaggcgcggg accttgcggg
     3961 agcggtcctc gcgcacgacg cggcgcgccg cggcgaggag ggccttcttc agcgcctcga
     4021 gcccgtcgcc cgtcacggcc gagcacgcga cgatcggcgc gtccgcgagg aacgagccct
     4081 tcacgagcgc gcggatctcc tcctcgacga gcgcgcccgt ctcggcgtcc acgaggtcgc
     4141 tcttcgtccg ggcgatcacg cccgtcgaga ccctgagcag cctcaggatg tcgagatgct
     4201 cgcgcgtctg cggcatcacg gagtcgtcgg acgccaccgc gaggaggacg aggtcgacgc
     4261 cctgcgcgcc cgcgaccatc gtccgcacga acttctcgtg cccgggcacg tccacgaacg
     4321 agaacgtcgt gtcgtcccag gccgcgtgcg cgaacccgag gtcgatcgtg atcccgcgcg
     4381 ccttctcctc ggggaggcgg tcgggatccg tcccggtgag cgcgttgacg agcgcgctct
     4441 tgccgtggtc gatgtggccg gcggttccga cgaggacgcg gcgcattaac ccgatcttgg
     4501 cacggcgacc caccgccatc gttgagggtc tatggtttcg tcctgggatg caccaccgtc
     4561 actccggggg atggccacgc ctgctgatgg tgtcgcgaca gagcttggat ggtggatgtg
     4621 taaagtccag cgccctcggt acccacccag agctgcggtt cggtcctgcc gtcgatcgcc
     4681 aacgagagaa ccgggacatt aggaagatcg ccggaaacat cgatccaata gcccgtctca
     4741 agcgacaggc ggtagattcg ccctaaccgg gaccgctggc ctgcgggcgc gcccgtggcc
     4801 gcgaacagat cgactgctgc ttcggtccta ccggcgagcg atgagatcca tgtttcgggc
     4861 aggcccaatg cgaggcgctc ccagctaatg ccgccgtcta cggtcttgaa aacaccgttg
     4921 ctcgtgccgg cgaagagggt tcccgagctg cgcgcgtcgg cgagaatggc ctgaatcctc
     4981 ggccgacctc ccccgtccag cccagcgttc gcttccgacc acgtctctcc accatccacg
     5041 gacttcacga tgccgaatgg aaaggtcgat agcccggcat agacggttcg ggagtcaagt
     5101 ggatcaacgg ctagcgccga aacgctcggc aggctcaggc cggaaaacgg ggcagcggtc
     5161 gcagaccagg agttcccaca gtccttcgat aggaagacgc ctgctgcctc gccgccggcg
     5221 tatgcggtgc atggagccga cggagagaat gcaacggaga tgacgggagc ggtctggtcg
     5281 ccgtgggcca tgccgatatt tgctgggcgc caggaagagc cgtcgtcgtc ggaaagggat
     5341 aacccgagcg gtgcgctggc gcctgcgaag acaggcgtcc cgatgtgcct tcccgccagg
     5401 acgaccggcg atgccaattg aaaggctggc gcgagtgttc cctcgtagcg ggtccagtta
     5461 gttccgccgt cggtcgaacg aaaaagtccg tcgccgacgg ccgaggtgat cacgctgccg
     5521 gcagtggtga cggtgatgcc gctatgcaat gaggccgcga gcgcggagga cttcaatgcc
     5581 ccggtcgcca cgtcgtacat cgcgacacct tcgattccgt ccgcgacgag ggctccctgg
     5641 tgtgttgaag tcagatccga aggaacatta tttccgatcc gcgtcgattg ccagctcgcg
     5701 ccagcatcta tgcttctcaa gagcgatacg ttcgctgcgg agacgacgac ggtgaagact
     5761 tcccgcgcgg ccgaccctcc tctaatgagg gcagcagggc cctcgaggac gggagcccat
     5821 gaggtcccgg aattcgtcga gcgaaaaagg cggccgccgg aatcggcggc ataaacggtt
     5881 ctcgagtccg tcgaatcgac cgcgacgggc cccgctggct gcgtcaacgc tgcaccggct
     5941 ggctgccagg tttcaccatg atccgtggac cggaaaacgg cgggcgtgcc tgcgaacccg
     6001 aatcgcccga acgcgacaaa gaccgtgccg tcctgcggcg caacggcaag gccccaaacc
     6061 tccggaaaaa gaccgacgga gccggccgga agcccggagc gtgccggttt ccacgacctg
     6121 ccgtcgtcgt cgctgcgaaa cacgccgaaa gcgtccgttg cggcgtacca ccttccggcg
     6181 gcatcagcct ctagaagcgt cacagagccc gcaggggaag cagtgggcgc cttccaggaa
     6241 agtccaccat ccgcgctaga aaagatgccg gaaggcgtgg tgacgaggac agacagagga
     6301 tttgcggggc tcacgcggac gatgctgacg cgatggtctg ggaggttggt cctcctccaa
     6361 gaccacgtca ttcctccgtc ggtgctgcga aagagcccgc ctagccctcc agcgagcgca
     6421 aaaccggcag gcgaaacgga aatcgatgcg atcggaccgc gatttggccc tttgccgatc
     6481 cactgtgcag ggaggggctg acaggccagc aagctgacca cgacagcccc tcgcgcgaaa
     6541 gctccggatg cgccgtgcat tcctaggggg acgcgactcg gttcacctgt ttgatggctg
     6601 cgacgttctg cccggaccaa agccctcgtc cctcaaagtc attgtaaaac tcatcgaagg
     6661 tgtcgtaacg atgcccgccc cagttgccga aaatatatgc ggagctgtac tgaaccgagt
     6721 ccacgggggg atttccgtcc ggccccgtcg cgttgacgct atcgaagacg tcccacagga
     6781 atgcgaatat attcatcgac actgaactgc cgccactcgc acatgatgaa taaaggtccc
     6841 cgttcgacga cggaatacct ccagggtttc cgctgaggaa cgtcgcatgc cagtcggcga
     6901 atgcttcggt gaagctcccc gaagcatttt gtggcgtgca ggagaggttg gtgccgccgg
     6961 cgtacgcgat ttgctccccc tgcacgagga actgcaggtt gtggccgaac tcgtgggtca
     7021 ttgtccagtc gtccagccac ttgaacggcg gctcaaagac gaccttaccg ccgaaaaagt
     7081 agggaacact gaagacggtg ttcgggtcgt agaccgcttt gaaggcggtg taagacgcca
     7141 gcccctgccc cgaccagtaa tcccgcaatt ctttgatctt gttgttccac tgaaagaacg
     7201 acgagtaagg agaaaatcca gagccgctag cggcgtccga aaacaccgga ttgaagaccg
     7261 ggctcccacc ggaccagtag aaccccgagt aaccgggccc cgcaggattt aggacctgct
     7321 cgacggtatt tagtggcagt gcatttccgt agcccacgtc aggcgcggta gcatggaact
     7381 cgatcaccgg attgtcgcca ccgggcggtt cagctgaata caccccaacg gcgtaagaac
     7441 catagtccga tatggtggtc tccgtgattc gggtaaacat gatggtgttt ccgcactcat
     7501 ccccaagttc gatctgcgtg taaatcctcg ccgggtaccc gggccgcagg gaatatgcac
     7561 ccccgccgta gttgtagaac gcggttcccg agaggctgtg atagtagttt tcgccgttgg
     7621 cgaacttgta gtcgccgcag ttggtctgag gccgtcgtcc agaatcccgc actgacgaag
     7681 tcaacctgtc ccaagcctgc tggctctgcg acgacggcgc tggaagtgag cgggccggga
     7741 aagacgcggt cgaccgtgcg accgcgctcg atacaccgac ccggccacga gcccgaagcc
     7801 tcgccatcgc ccgcgctgca agggcgtcac ctgccgagac cagctcttgc tcgctcgccc
     7861 gcgccgccat cgcctcgaac tctccctctg cgacaaaggt catggtgccg ccggctgcca
     7921 agaagcggaa cgtccgagac gcgacgagtg cagttttctc cctgaccgag acctcgattg
     7981 acagctcaga gcaggtgttg catgaaagcg ggacgaggac gtccttggac gttgcccctt
     8041 caaggctctc gacgcgtgtc agcacttcct ctcccgaggg gccggtgacc tgacgaaagc
     8101 gagcgaaggt attgccggag atgggcgcag tcgcgctgaa agaaacgacg agcagggccg
     8161 attcgccgtt ttctggtctt tgacacttgc cctgaatgtt gatccgtggg ggcgaggggt
     8221 ggggaggcat ggccagtagc ggcccccgaa atccgttacg acgaagatga tggctacaaa
     8281 gattctgacc ggatggaatc gcatcttcac ctctttgtct tgatcggggt ggaacgactg
     8341 acgaaccgag ttgacgcgtc agtccgaaga tgcgggtcgg agcgaggatt tgtgcgttgc
     8401 gatttgagga caggcgtctt caccttgata agcagtcgaa agctgccgac ccagcgaccg
     8461 aaccgtggta tggaattcgc gtcgtgcgaa ttgcgcgagc ccggtcgggc tcgctagacc
     8521 gagagcccgt gccagatcag cgagaggtag agaggcctct cgttgtagga ggttcaagag
     8581 gagagcggcc cgccgagcaa cagagacgct gcggagagaa agatccgcct tagaggcgcg
     8641 gcgagacaac gttcgtaccg acacaccgtg catcgctgcc agaccctttg aacccagctt
     8701 aggcaagagg tcgaggagat cggttcgaag ttcggcgaga ggacaaggag tatgcgcgcg
     8761 acgatcgctg cgtggattgc gaggccccgc gcacatgccg aattagaaga gccttgaacg
     8821 cctagctttt cagtaatgca cccgcatcag tattcggggc ccttgaagat cgtggacttc
     8881 tcgggctcga gcctctcgcc cgcgagccgc gcgcgcacgt gccgcgcgac gcgcaggccg
     8941 tccagcgcgg ccgagacgat gccgcccgcg aagccggcgc cctcgcccgc ggggtagagc
     9001 ccgggcacgg acgtgctctc gcaccagccg tcgcgctcga tccggacggg cgacgacgtg
     9061 cgcgactcgg gcgcgaagag caccgcctcc tccgacacga agccgggcat cttccggtcg
     9121 aacgccttga gcccctcggc gagcgcctcg aggtaaggct cgggcaggat cccgtcgaag
     9181 gacacgggag tcacccaggg gccgtacgtc gtcttcggga gcgggcgcgg cgagatgggc
     9241 cgctcgcggc tcctgaggaa atcggtcacg cggagggccg gcgccgcgta cgtcccgccg
     9301 gcggccgcga acgccgcggc ctcgacgcgc cgctgcagct cgcgcccgcg caggagctcc
     9361 ggccccggca tcgcgtcgaa gtccacgtcc ttcgtctgga cgacgatccc ggcgttcgcg
     9421 tagcccgagc tgcgcttgta gcgcgacatc ccgttcgtga cgacgccgcc cggctcggac
     9481 gagcacgcga tgacctcgcc gccggggcac atgcagaacg tgtagatccc gcgcccgttc
     9541 gaggcgacgt gcgcctgctt gtagtccgcg ggctggacgc cgcactccgt cgacacctgc
     9601 gcgtactgga tgaggtcgac ggcgtcctgc gggtgctcga tccgtacgcc catcgcgaag
     9661 tgcttgcgtg tcatcgcgac gccccgggcg ttcagcacgt ccatcgtgtc gcgcgccgag
     9721 tggccgagcg cgaagatcgc gacgtcggcc ggaacgtcct cgccggactc gagcgtgagg
     9781 cccgcgagcg atccgtccgg ccgggtcctc aggtccgtga ccttcgcgcg atagcgcagc
     9841 gtcgccccgt gctcgaggag gtgcttgcgg atgttgcgca ggacgacgac gaggcggtcc
     9901 gttccgatgt gcggcttcgc gtccacgaga atggagtcgg gcgcgccgaa gcggtggaac
     9961 gtctcgagga cgaccttgac cttgtcctgg cgttttccgc acgtgagctt gccgtcggag
    10021 aacgtccccg cgcccccttc cccgaactgc acgttcgact cggggtcgaa gacgcccgac
    10081 gtccagaact tcatgacctt gcggtagcgg tcctcggccg ggtcgccgcg ctcgaggatc
    10141 gtgaccggga ctcccgcgtc cacgagggcg agcgccgcga agaggccggc cgggcccgtc
    10201 ccgacgatcg cgaccttcgc cttcgtgtcc agctcgggca ggagcggcgc cggcgccggc
    10261 gggggctcgg ccggcgagag gcggagcggg gcctcgggcg tcaggtcgag gtccgtcgcc
    10321 gacgtgaact cgaccgcgat ctcccagtag aggacgggct tctggcgcgc gtcgagggag
    10381 cggcgcacga gggcgaagtc ggagaggcct tcccggggaa tgccgaggag ccgcgcgacc
    10441 gtcgcgaggt tcacgatctt cggggtgcgc gcacggacac ccgcgaggcg atagcgcggc
    10501 acgcgcgtat tgtgccgccg cgctactctg atgtcatgca cggcccgcgc gaaatcgaga
    10561 cgaaattccg ggtgagagac cgcggagcgc tggaggagcg cctggcgggc ctcggcgcgc
    10621 gtccgggccc gcgggaggac gagacgaacg tcctctacga cgaaccgggc ggccggctga
    10681 gggcagaggg atgcgcgctg cgtcttcgga ccgtgaacga gcgcggcctc ctgaccttca
    10741 agggacctcc ggaggtctcc ggcggaatca agagccgcgt cgaatgggag agcgaggtcg
    10801 attcgccgcg ggccgtcgcc ggcatcctcg agtccctcgg ctaccggcct tctttccggt
    10861 accacaagct gcggacgacg tggaacttct cggatcccgc tcggcccgtc gtcgtcgtgg
    10921 acgagatccc gctcgggctc ttcgccgaga tcgagggccc cgatcccgcc gttcgggccc
    10981 tggccgccga gctgggcgtc gcggaggcgg acttcatcgc cgagtcgtac gtcgcgctct
    11041 ggctcgcggc gcgcgcgaag gacccgtccc tgccggaaga catggtgttc ggccgatgaa
    11101 agccctcgtc ctcgccgccg gcttcggaac gcgcttccgg cccgccacgc tctcgacgcc
    11161 caagcctctc ctcccgctcc gcggccggcc gatcctcttc cacgtcttcg accatctcct
    11221 cggcgagggc gcggaccgct ttgtcgtgaa cgcgcaccac ctcgcccgag agctgcacgc
    11281 ggccgtcggc gactcgtacg caggcgttcc cgtcgactgg tccttcgagg agaggatcct
    11341 cggcacggcg ggcggaatcc ggtgcgcctt cgaacgcggc cgcctcggcg atcacttcct
    11401 cgtcgcgaac ggcgacgtcg cgacgacgct tccgctcggg cgtctcctcg cggcgcggca
    11461 cgagccgggc gtcgtgtcgg ccctcgccgt cctcccgaac gaggaccccg aacgcgacac
    11521 gccgctctgg gcggacgcgg cgggccggct cgtggccgtg ggcggcccga ggcccgggag
    11581 cgcgacgggc ccgtggctct tcacgggcct tcaggccgcg acggccgcgc tcgctcttcg
    11641 catccccgaa ggcttcgccg agctcgcgaa ggacgtcctc gtcccgtcgg cggccgcggg
    11701 ggacggcgcg ttcgcgctcg tcccgtacac gaaggcggac gggctctggt tcgacctcgg
    11761 cacacccgag cgcctcgcgg cggccgaggc agcgctggcc gctgcgagcc gccccgaacc
    11821 ggacgcgacg agaggctaga agacgcctct ccggagcgcg gcgaagcccg cggccgcgag
    11881 aaggagtccg gtcgcgagca gcgcccatgc ggacagcgtg gggatcggag aggtggccgt
    11941 cccgttgacg gagccggcgt tcgtggaacg ggagggcgcg gcagggctgc cgcaggtcgc
    12001 gttcgcacag gacgcttcca gccccgacga gaggttgacg aggccgccgt tgctcgacag
    12061 cggcgacgcg aagaacaggg cgaacgcccg tgtgccggtc aggttcggca cgttgctcgc
    12121 gccggcgagg aacccgctgg acgtcgggga gacgcccgga gaaacgaata ggaacgtggc
    12181 cccggtcgcg agcacggttc ccggcgtcgt tgtgatgttt acggagctgt acgtgttcgt
    12241 ggacgagtcg tagacgaacg agccggatgc cgtgcctccg tccgcgaacg tcacgccgtt
    12301 gagctgccag acgacgggga tcgcgtcggc gtttccggag aggaaacaga gggcgaggag
    12361 cagtagcgcc gctttgcgca tgaaacctcc accacgctca ggatcgagtg cgggaagact
    12421 atcagagaaa aaggaagacg acgtctcacc accgcgcgat ccatttcagc ttcttcccgt
    12481 gccaggtcca gatctggatc ttcgcgttga tcggggtgac gcgcacggcg gcctgccggt
    12541 tcctgcggtt cgagaggtac accgtcccgg gagacgcgtg gcccacgggc gagaaggtgc
    12601 agatgttcga ccttccgaac cgcaccgggt cggacaggtt cccgatgggc gcgcctccgg
    12661 gatccgtccc gtccatcccg ggcacgaacg agaaggcgac gcccgggtaa cggctgccca
    12721 gccagtacgg gcccgcgacg agcctgtcga cgcccttccg gatgtcggcc gtcgtcacgc
    12781 cgttggcgtt gccgtcctgg tagacggtca ggacgaggtc gccggcgttc gcgacccact
    12841 tcacgccgac gtcgacgccc tggaagacgg cgcgggcgcg ggcggcgtgg aggatcgcgg
    12901 cgacctcgcg cgcggcggtg gcgagcgact cctcggcgag gatctccgac aacgcaggga
    12961 tgctgatcag gaagagggcg ccgaggatgg cgaggacgac ggcgagctcg gccagcgaga
    13021 cgccgcgttc ggatgcgcgc atggttcctc ccttcgtgcg cgtgaggagg ggggacggcg
//
LOCUS       DAOMFY010000090        12994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1472_length_12994_cov_7.657006, whole genome shotgun sequence.
ACCESSION   DAOMFY010000090 DAOMFY010000000
VERSION     DAOMFY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12994
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_12994_cov_7.657006"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="PLB01_09180"
     CDS             <1..1446
                     /locus_tag="PLB01_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77513.1"
                     /translation="TTTTGTAIDIQGTGNTGTMSFTSVSANGGTAGIVLSNFTGGSFT
                     VTGAGGACGPADTTCSGGTIQNMTGGDDSSAVPVGSGVVLNNVASVSLTRLRIRSNSN
                     YGLRGTSVAGFTMDTCFLDGTTGTNGATPYNDGAVSFDNLTGSATVSNSNIQGGFSNN
                     FRLVNTSGTLNRITFSNVTFDVSGASPANDALTIESQGAAVVNVTVQNSFFKSAAGDL
                     FQLNNIGTGADDLVFTGNTLTNGNSAIATGGGGVTIGSNGTGNITSNFSNNTLRDAVG
                     HAILFFKSTGSATVTGTFDNNTIGVSGAANTGSAEGDGIKVQNAGQGTVKVTITNNQI
                     RQYNVFGIELLTGGGASAMPGALNATITGNTIAQPGNTAGTIGLPKNGIHVNDGTVVG
                     DTYQVCADIVSNSMTGSGADAVPPTGIVADFRLRQRQATTLRLPNYAGANNDNAAAVA
                     FVSGNNGGASGLASNTVPTGGGFTGGAGSCP"
     gene            1477..1977
                     /locus_tag="PLB01_09185"
     CDS             1477..1977
                     /locus_tag="PLB01_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HQQ77514.1"
                     /translation="MAEPFLAEIRIMSFSFPPKGWALCNGQLLPINQNQALFSLLGTT
                     YGGDGRVNFALPNLQGQVPIHMGSGHTLGERGGEQAHTLTISELPTHTHVVGASSLAQ
                     GGTASPSGSVLGGANNAYHPPTNLTALNPASLLNVGGSQAHLNMQPFLVLNFSIALQG
                     IFPSQT"
     gene            1988..2491
                     /locus_tag="PLB01_09190"
     CDS             1988..2491
                     /locus_tag="PLB01_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HQQ77515.1"
                     /translation="MAQPYVGEIRIFAGNFAPAGWMFCEGQLLPISEYETLFNLIGTT
                     YGGDGQSTFALPDLRGRVPLHFGNGFVLAETGGVETVTLTVSQIPSHSHPVLASTSPG
                     GQTSPGGNVLAASFNVTPYINDVPNGTMSANAVGSVGGSQPHNNFQPYLCVDFIISLF
                     GIFPSQT"
     gene            2502..3020
                     /locus_tag="PLB01_09195"
     CDS             2502..3020
                     /locus_tag="PLB01_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HQQ77516.1"
                     /translation="MDPFVAEIRIFPFNFAPKGWAFCDGQILPLSQNTALFSLLGTTY
                     GGDGKSNFALPNMQGNAPMHPGQGPGLSLHDLGETGGSETVSLLESEIPSHSHNLRAD
                     VVDLADTNVPSPAASLALSSGGTLYQGTPNTQMDGQMLAPAGGDQPHNNMQPYLTLNF
                     CIALQGVYPPRT"
     gene            3028..3522
                     /locus_tag="PLB01_09200"
     CDS             3028..3522
                     /locus_tag="PLB01_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ77517.1"
                     /translation="MTKVIALRPIRPEDRDLLCRIYGSTRTEELAPVPWTDEQKAAFV
                     RQQFDAQTSYWDEQYPEAERFVVEVDGTPAGRLYVQRWPKEIRLVDIALLPDFRGRGV
                     GTELIQGLFSEAARDGKPVTIHVEIFNPARVLYERLGFAPKGEQGMYVLMEWKPEAVG
                     AGVS"
     gene            complement(3515..3811)
                     /locus_tag="PLB01_09205"
     CDS             complement(3515..3811)
                     /locus_tag="PLB01_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77518.1"
                     /translation="MLGELTYSNFFERVGQEFRIAAGEGVAVLVLTEVTDLARPESPS
                     PRRKPFSLVFRGPRAPVLPQRIWPLEHAALGRLEIFLVPIGPDAEGMRYEAIFN"
     gene            3851..4276
                     /locus_tag="PLB01_09210"
     CDS             3851..4276
                     /locus_tag="PLB01_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77519.1"
                     /translation="MKTTRAQFLTTLATGVAAAALGPSSLLGAGPDVPDGRAFKALVG
                     ETFRFRGFERRDPVDLVLTDYSERALRGSTRQFTLTLVAPGGERLREATYTVDHPKAG
                     TFEMFVIPAGFDAKAQPLYRADFNLLVAVSGAPTTVQRR"
     gene            complement(4278..4889)
                     /locus_tag="PLB01_09215"
     CDS             complement(4278..4889)
                     /locus_tag="PLB01_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77520.1"
                     /translation="MKVVFDSVALSGGMLDAPIRLLNESGADLFALRIDLAGVTESVK
                     PQGQAAFSRDQDVAPPPPLAFDAIRSGAETPGGLFRGGPLSFSEDTEVVVVLGVVSGV
                     AAVPSNPKVDVRTPSTKASACPGAAPACRVDAEGNVWRVEPAEGEKPGGLSERSRDGG
                     VIRSLRFERGDRPVDLSIAPDGRLLVFFDDGSKDGAVRAFRPF"
     gene            complement(5099..5587)
                     /locus_tag="PLB01_09220"
     CDS             complement(5099..5587)
                     /locus_tag="PLB01_09220"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HQQ77521.1"
                     /translation="MSIRILVTGGTFDKEYDELTGRLFFKETHIHEMLRLGRCREPVT
                     VETVMMVDSLDMTDDCRARIADACRRAPEDRIVITHGTDTMVETAAVLAKEPTAKTIV
                     LTGAMVPWAFGSSDGLFNLGSAISFVQILPPGIYVAMNGRYFDWHDVRKDRAAGTFET
                     VK"
     gene            complement(5602..7281)
                     /locus_tag="PLB01_09225"
     CDS             complement(5602..7281)
                     /locus_tag="PLB01_09225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQQ77522.1"
                     /translation="MKKTPLDPSFAGLGLDARLVRALAALGYEEPTPIQSASIPPMLE
                     SRDVLAQAATGTGKTAAFALPILHRIGAAEKRGTAPMALVLVPTRELAMQLAEAFHRY
                     GREIGVRVLPVYGGASMVAQLNALKRGVDVVVATPGRALDHVNRRSLALDGLVALVLD
                     EADEMLDMGFAEDLDAILAATPKTRQTALFSATLPARIGAIAAKHLKDAVRITIAKDS
                     VTPGKLPKVRQTAYVVARGHKRAALGRILDLESPAAALVFCRTRLEVDDLTESLKGRG
                     MRVEALHGGISQEGRDRVMKKLRTGALELVVATDVAARGLDISHLSHVVNYDVPSSPE
                     VYVHRIGRTGRAGREGVAITLAEPREHWQLKNIERLTKQRIEVANVPTVADLRAKRLE
                     LTRASLEEAIVEGGLEHYRVVVESLAEDHDPMDVAAAAVRLIERASGAPGEDEPEIPA
                     VAVPPPSRRASPAAGAGPGRYKFGGRDSGTSARLFVGAGRAAGMRPADLVGAIANEAK
                     ANPRDIGAIQIADRFSLVEVPEAIAETVIRALRNATIRGQKVVVRRDRDKA"
     gene            7340..8188
                     /gene="kdsA"
                     /locus_tag="PLB01_09230"
     CDS             7340..8188
                     /gene="kdsA"
                     /locus_tag="PLB01_09230"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQQ77523.1"
                     /translation="MLRVKVFASITPHLSVGDRCRPVFFAGPCVLESRAHALKMAKAI
                     KQVFADCGAADRLVFKSSFDKANRSAGASFRGPGIEKGLSILSEVKERTDLPILTDVH
                     EPEQCAIAAEVADVLQIPAFLCRQTDLIEAAAATKKAVNVKKGQFLAPDDCKQIVDKC
                     RAKGNTNVTLTERGTTFGYHNLVVDFRGLPMMRALGVPVIYDATHSLQLPGGLGKETG
                     GSREYAPALARAAAAVGVDGYFFEVHDDPAHAKSDRATQLPLASLPQFLSALLSFDQL
                     RRETIG"
     gene            8269..8676
                     /locus_tag="PLB01_09235"
     CDS             8269..8676
                     /locus_tag="PLB01_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQQ77524.1"
                     /translation="MSRGLSVDPTDAVPLWKQIEENVRRLVASGALKPGAAVPSVRDL
                     ARELSINPATVAKGYQRLVDAGILAVKRGEGTFVAEKPPAISRGDRDKELEGGALRYA
                     SLAVTLSASNEEAVEHVKRSIGFLRRKREGGAE"
     gene            8673..9617
                     /locus_tag="PLB01_09240"
     CDS             8673..9617
                     /locus_tag="PLB01_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ77525.1"
                     /translation="MTTQQRGDAPVVEVRSLLVRYGGNPKPTLEEISFSVPRGSVYAL
                     LGRNGAGKSSLVRCLLGQQKPTAGGCFLFGEDVWKRRASLMAKVGVVPEDPDAPPAMT
                     ARQLSAFCAKLYPVWDAPSVEARLARFGVPAGTPYGSLSKGQRALVSLALALASSPDL
                     LVLDDPTLGLDAVARRAFFEELVGELADRGTTVLLTSHDLAGVESMATHVGFLKDGKL
                     AVDEELETLKGRFRRLRYANEQTEERTEYGQELAAFDAVRVKVRGWGIEAIVSNYGEE
                     SFTRFSSLEGVVDAESSSLSLEEIFVAVAGESPAGRNA"
     gene            9614..11587
                     /locus_tag="PLB01_09245"
     CDS             9614..11587
                     /locus_tag="PLB01_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77526.1"
                     /translation="MKAFAAVFAREVFERRMVFLVGFCCGIPTLVVSALHGWNTPGNA
                     EVRLLAASVGGLALAVALALLYGGSMIPGETAERRISFWFSRPIPAAAIWAGKLLAAI
                     FVSLSAGFLVFLPGWLTGPAPTRALWGIDLLAGQAALAALAVVAGCVFASHAVVTVAR
                     LKSPWALLDLTLAITLAFAATFLVRSLVRDHLGDPTYGLEAGPAWIGSAMLAGAVALA
                     FPLATLAQVAAGRADARRAHGAFSFVLWAVLGSGVLAVGAFAWWCASARATDLRQVAG
                     NVASARRGSWTAVGGALGGARGGASFLFDTASLASIRLRGCGETFSQNGARAAWGEPD
                     FGILDRRSNRMELVVADLASGRAVATGIVSADWPWLTLSPSGHRLASLAGLTISAYDV
                     SEPANPKQLATFRLEHAMPRFAFVDEDTIRVFPRLSGTTKPNLAAIDAEIRDLSLSSK
                     KSLVTGRLDLESLSWLRLSADGRFLVGTRRATEDASGPRTVMLLDGRTGARVATLAEG
                     LQSAQARLLVGNRIVVAGIAKAGARALFFEGERGWSAPARTVELGPAVRITLGGEIDT
                     GRVAISLFPFEEETKAHPRAKLVVVDAASGAVSQLGEGLVPVDRMGWWIDPVMTPPEA
                     GAPWTSLFLDADGRLVRLDPATGKQTVLLGKGK"
     gene            11599..12564
                     /locus_tag="PLB01_09250"
     CDS             11599..12564
                     /locus_tag="PLB01_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQQ77527.1"
                     /translation="MTTPARQQALRVIEIEAGAVRALADGLDEGFDRAVAAMKSTAGR
                     VVVTGIGKSGIVGQKIAATLASTGTAAFYLHPSEAIHGDLGMILGGDVVLALSHSGET
                     EEVVALIPHVKRRGATLVAMTGRRDSTLGKGADVVVSITIREEACPLNLAPTASTTAQ
                     LVMGDALAMALSVEKGFQPEDFAALHPGGKLGKRFLKVSDLMHAGADVPLVASAAPMK
                     DVVYEMSRKRFGITGVTEGGKLVGAVSDGDLRRLLEKDGPKIMNDSAADAMGRSPKTI
                     AKDAWATEALRIMEERKITSLFVVAPDGRPEGILHLHDLWGVESI"
     gene            12593..>12994
                     /locus_tag="PLB01_09255"
     CDS             12593..>12994
                     /locus_tag="PLB01_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQQ77528.1"
                     /translation="MPAAPIVPDDLRTKLSRIRGLVLDVDGVLTDGSMTFDEDGRETK
                     TFDVKDGLGLVLLRDLGYRIGVISSRTSRVVSRRCADLKIPEEFVLQGERDKAAAFDL
                     LAKRWDLEAWEIAAVGDDLPDLGMLRRAGVGA"
BASE COUNT         2002 a         4664 c         4381 g         1947 t
ORIGIN      
        1 acgacgacga ccggaacggc gatcgacatc cagggcacgg gcaatacggg gacgatgtcg
       61 ttcacgtccg tcagcgccaa cggcggaacg gccggaatcg tcctctccaa cttcacgggc
      121 ggcagcttca ccgtgaccgg ggcgggcggc gcctgcggcc ccgcagatac gacgtgctcc
      181 ggcggcacga tccagaacat gaccggaggc gacgactcct cggccgttcc ggtcgggtcg
      241 ggcgtggtcc tgaacaacgt cgcgagcgtc tccctgacac gcctgcggat ccggtcgaac
      301 tcgaactacg gcctgcgcgg cacgagcgtc gccgggttca cgatggacac gtgcttcctc
      361 gacgggacga ccgggacgaa cggcgcgacg ccgtacaacg acggggcggt ctccttcgac
      421 aacctgacgg gatccgcgac cgtctcgaac agcaacatcc agggcggctt ctccaacaac
      481 ttccggctcg tgaacacgag cgggacgctg aaccggatca cgttctcgaa cgtcacgttc
      541 gacgtgagcg gcgcgagccc ggcgaacgac gcgctcacga tcgaatccca gggcgccgcg
      601 gtcgtgaacg tcacggtcca gaacagcttc ttcaagagcg ccgcaggcga cctcttccag
      661 ctgaacaaca tcgggacggg cgcggacgac ctcgtcttca cgggcaacac gctcacgaac
      721 ggcaacagcg ccatcgcgac gggcgggggc ggcgtgacga tcggaagcaa cggcacgggc
      781 aacattacgt ccaacttctc gaacaacacc ctccgcgacg ccgtgggcca cgcgatcctc
      841 ttcttcaagt ccacgggctc cgccaccgtg accggcacgt tcgacaacaa cacgatcggc
      901 gtttcgggcg cggcgaacac gggctccgcc gagggcgacg gcatcaaggt gcagaacgcc
      961 ggccagggca cggtgaaggt cacgatcacg aacaaccaga tccgccagta caacgtcttc
     1021 ggcatcgagc ttctgacggg aggcggcgcg tccgccatgc cgggcgccct caacgcgacg
     1081 atcacgggga acacgatcgc gcagccgggc aacacggcgg gcacgatcgg gcttccgaag
     1141 aacggcatcc acgtgaacga tggaacggtt gtcggagaca catatcaggt gtgcgccgat
     1201 atcgtctcga actcgatgac ggggagcggg gccgacgccg tgcctcccac cggcatcgtc
     1261 gccgacttcc gcctgcggca gcgccaggcg accacgctcc gcctgccgaa ctacgccggc
     1321 gcgaacaacg acaacgcggc cgccgtggcg ttcgtgtccg gcaacaacgg cggcgcctcg
     1381 ggactcgcgt cgaacacggt ccccaccggc ggcggcttca cgggcggggc cgggagctgc
     1441 ccgtgagcac atcgtcacag agccaaggag gtttcaatgg ctgagccctt cctcgccgag
     1501 atccggatca tgagcttcag cttcccgccc aagggctggg ccctctgcaa cggccagctc
     1561 ctgccgatca accagaacca ggctctcttc tcgctgctcg gcacgacgta cggcggcgac
     1621 gggcgcgtga acttcgccct gccgaacctc caggggcagg tcccgatcca catggggagt
     1681 gggcacacgc tcggcgagcg cgggggcgag caggcgcaca cgctcacgat ctcggagctg
     1741 ccgacgcaca cgcacgtggt cggcgcctcc tctctggccc agggcgggac ggcgagcccg
     1801 agcggcagcg tcctcggcgg cgccaacaac gcctaccacc cgcccacgaa cctcaccgcg
     1861 ctcaatccgg cgagcctcct gaacgtgggc ggttcgcagg cgcacctcaa catgcagccg
     1921 ttcctcgtcc tgaacttctc gatcgccctc cagggcatct tcccgagcca gacctaggag
     1981 gcatgcaatg gcgcaaccct acgtcggcga gatccggatc ttcgcgggca acttcgctcc
     2041 cgcgggctgg atgttctgcg aaggccagct cctgcccatc tccgagtacg agacgctctt
     2101 caacctcatc ggcacgacgt acggcggcga cgggcagtcg acgtttgccc tccccgacct
     2161 gcgcgggcgc gtgccgctcc acttcggcaa cggcttcgtt ctcgccgaga cggggggcgt
     2221 cgagacggtc accctcacgg tctcccagat cccgtcgcat tcgcatcccg tcctggcgtc
     2281 cacctcgccg ggcggacaga cgtcgccggg cggcaacgtc ctcgcggcgt cgttcaacgt
     2341 gacgccctac atcaacgacg tgccgaacgg aacgatgagc gcgaacgcgg tcggctccgt
     2401 cggcggcagc cagccgcaca acaacttcca gccgtacctc tgcgtcgatt tcatcatttc
     2461 cctgttcggg atcttcccga gccagacctg aggagagccg catggatccc ttcgtcgccg
     2521 agatccgcat cttcccgttc aacttcgccc cgaagggctg ggcgttctgc gacggtcaga
     2581 tcctgccgct ttcgcagaac acggcgctct tctcgctgct cggcacgacg tacggcggcg
     2641 acggcaagag caacttcgcc ctgccgaaca tgcagggcaa cgccccgatg caccccggcc
     2701 agggtccggg gctgtcgctc cacgatctcg gcgagacggg cggttccgag acggtctcgc
     2761 tcctcgagtc cgagattccc tcccactcgc acaacctcag ggcggacgtc gtggatctcg
     2821 ccgacacgaa cgtgccttct cccgcggcgt cgctcgccct ctcgtccgga ggaaccctct
     2881 accaggggac cccgaacacg cagatggacg gccagatgct cgcccccgcg ggcggcgacc
     2941 agccgcacaa caacatgcag ccgtacctga cgctcaactt ctgcatcgct ctccagggcg
     3001 tctatccgcc gcggacctga cgccgccgtg acgaaggtga tcgcgctgcg cccgatccgc
     3061 ccggaggacc gcgacctcct ctgccggatc tacggctcga cgcgcacgga ggagctcgcg
     3121 cccgttccgt ggacggacga gcagaaggcc gccttcgtcc gccagcagtt cgacgcgcag
     3181 acttcgtact gggacgagca gtatccggag gccgagaggt tcgtcgtcga ggtggacggc
     3241 actcccgccg gccgcctcta cgtccagcgg tggccgaagg agatccgcct cgtcgacatc
     3301 gcgctcctgc ccgacttcag ggggcgtggc gtgggcaccg agctcattca aggtctcttc
     3361 tccgaggccg cccgggacgg aaaacccgtc acgatccacg tcgagatctt caaccctgcg
     3421 cgcgtcctct acgagcggct cgggttcgcg cccaaaggcg agcaggggat gtacgtcctg
     3481 atggagtgga agcccgaagc cgtcggggcc ggtgtcagtt gaagatggcc tcgtaccgca
     3541 tgccttccgc gtcgggcccg atcggcacga ggaaaatctc gagcctcccg agcgccgcgt
     3601 gctcgagcgg ccagatgcgc tgcgggagaa cgggcgcccg gggaccgcgg aagaccagcg
     3661 aaaacggctt gcggcgcggc gacgggctct ccggccgtgc gagatccgtg acctcggtca
     3721 gaacgaggac ggcgacgccc tcacccgccg cgatgcggaa ctcctgtccg accctttcga
     3781 agaaattcga gtacgtcagc tccccgagca tccatcggat cgtggcacag aacccgcctg
     3841 gaggcacccc atgaagacga cgcgcgcgca gttcctcacg accctcgcga ccggcgtggc
     3901 ggccgccgcc ctcggcccgt cctcgctcct cggcgcggga ccggacgttc ccgacggccg
     3961 cgccttcaag gcgctcgtcg gcgagacctt ccggttccgc gggttcgagc gccgcgatcc
     4021 ggtggacctc gtcctcacgg actactcgga gagagcgctg cgcgggagca cccggcagtt
     4081 cacgctcacg ctcgtcgcgc ccggcggcga gaggctccgg gaggcgacgt acaccgtcga
     4141 ccatccgaag gccggcacgt tcgagatgtt cgtgatcccc gcggggttcg acgcgaaggc
     4201 ccagccgctc taccgagcgg acttcaacct gctcgtcgcc gtgtcgggcg cgccgacgac
     4261 ggtccagcgc cgctagatca gaacggccgg aacgcgcgca ccgcgccgtc cttcgacccg
     4321 tcgtcgaaga agacgagcag acgcccgtcc ggcgcgatcg agaggtcgac gggcctgtct
     4381 ccgcgctcga accggagcga gcggatcact ccgccgtcgc ggctgcgttc ggagagcccc
     4441 cccggcttct ctccctcggc gggctcgacc cgccagacgt ttccctcggc gtccacacgg
     4501 caggccggcg cggcgcccgg gcacgcggag gccttcgtcg agggcgtccg gacgtcgacc
     4561 ttcggattcg aaggcaccgc ggcgacgccc gacacgacgc cgaggacgac gacgacctcg
     4621 gtgtcttccg agaaggagag aggacctccg cgaaagaggc ctccgggagt ctcggcaccg
     4681 gagcgaatcg catcgaaggc gagcggaggc ggaggcgcga cgtcctggtc gcgcgaaaac
     4741 gcggcctgtc cctgcggctt cacgctctcg gtcacgcccg cgaggtcgat ccgtagggcg
     4801 aacaggtcgg ctccgctttc attgaggaga cgaatcgggg cgtcgagcat ccctccggaa
     4861 agcgcgacgg agtcgaagac caccttcaag gaattcttcg tcccgggtac ccgaagcgcg
     4921 aagggtccac gagttatcgc gcggcccctc gcgtcgaacg acaacgcgaa cgtcagcccg
     4981 cgcgagaccg gcgccggcgc ggacaccgcg cgagccgctg caagaagcgc gaggccgagg
     5041 cccgcgcacg ggaccattcg gagaatcttc ggccgaggga tcttcatccc ggcgcctttc
     5101 acttcacggt ctcgaaggtc ccggccgcgc ggtccttccg cacgtcgtgc cagtcgaaat
     5161 agcgcccgtt catcgcgacg tagatgcccg gaggcagaat ctggacgaac gagatcgcgc
     5221 tcccgaggtt gaagagcccg tccgagcttc cgaacgccca gggcaccatc gcgccggtca
     5281 ggacgatcgt cttcgcggtc ggctccttcg cgaggaccgc cgccgtctcg accatcgtgt
     5341 ccgtgccgtg cgtgatcacg atccggtctt ccggcgcgcg ccggcaggcg tccgcgatcc
     5401 gcgcgcggca gtcgtccgtc atgtcgaggc tgtcgaccat catcacggtc tcgaccgtca
     5461 cgggctcgcg gcagcggccg aggcgaagca tctcgtggat gtgagtctcc ttgaagaaga
     5521 gccgtcccgt gagctcgtcg tactccttgt cgaacgtccc gcccgtgacg aggatgcgga
     5581 tggacatgcg ccgtttccct ctcaggcctt gtcgcggtcg cgccgcacga cgaccttctg
     5641 gccccgtatc gtcgcattcc ggagtgcccg gatcacggtc tcggcgatcg cttcaggcac
     5701 ctcgacgagc gagaagcggt ccgcgatctg gatcgcgccg atgtcgcgcg gattcgcctt
     5761 cgcttcgttc gcgatcgcac ccacgaggtc cgccggacgc atcccggccg cccgtcccgc
     5821 gcccacgaag aggcgcgccg aagtcccgga atcgcgccct ccgaacttgt agcgcccggg
     5881 tccggctccg gccgcgggag acgcccggcg cgacggcggc gggacggcga cagccggaat
     5941 ctcgggctcg tcctcccccg gcgccccgga ggcgcgctcg atcagacgca cggcggccgc
     6001 agcgacgtcc atcgggtcgt gatcctccgc gagggactcg acgacgacgc ggtagtgctc
     6061 gaggccgccc tcgacgatcg cctcctcgag cgaggcacgc gtcagctcga ggcgcttcgc
     6121 gcggaggtcg gcgaccgtcg ggacgttcgc gacctcgatc ctctgttttg tgaggcgctc
     6181 gatgttcttc agctgccagt gctcgcgcgg ctcggcgagc gtgatcgcga cgccttcgcg
     6241 ccccgcgcgc ccggtccggc cgatgcggtg aacgtagacc tcgggcgagg acgggacgtc
     6301 gtagttcacg acgtgcgaga ggtgcgagat gtcgaggccg cgtgccgcga catccgtggc
     6361 gacgacgagc tcgagcgcgc ccgtgcggag cttcttcatg acgcggtcgc gcccctcctg
     6421 cgagatgccg ccgtggagcg cctccacgcg catcccgcgg cccttgaggc tctccgtgag
     6481 gtcgtcgacc tcgaggcggg tgcggcagaa gacgagggca gccgccgggc tctcgaggtc
     6541 caggatgcgg ccgagcgccg cgcgcttgtg cccgcgcgcg acgacgtatg ccgtctggcg
     6601 caccttcggc agcttgccgg gcgtgacgct gtcctttgcg atcgtgatgc gcacggcgtc
     6661 cttcaagtgc ttggccgcga tcgcgccgat gcgcgccggg agcgtcgccg agaagagcgc
     6721 cgtctggcgc gtcttcggcg tcgccgcgag gatcgcgtcg aggtcttcgg cgaagcccat
     6781 gtcgagcatc tcgtccgctt cgtcgaggac gagcgcgacg aggccgtcga gcgcgagcga
     6841 cctgcggttc acgtggtcga gcgcccggcc cggcgtcgcg acgacgacgt cgacgccccg
     6901 cttcaaggca ttgagctgcg ccaccatcga cgcgccgccg taaacgggga gaacgcgcac
     6961 gccgatctcg cgaccgtacc ggtgaaacgc ctcggcgagc tgcatcgcga gctcgcgcgt
     7021 cgggacgagg acgagggcca tgggagccgt cccgcgcttc tcggcggctc cgattcggtg
     7081 gaggatcggc agcgcgaacg cggcggtctt ccccgtaccg gtggccgcct gcgcgagcac
     7141 gtcgcggctt tcgagcatgg gcggaatcga cgcgctctgg atgggagtgg gctcctcgta
     7201 ccccagggcc gcgagggccc ggacgagccg cgcatcgagg ccgaggccgg caaaggaggg
     7261 atcgagcggc gtcttcttca tgcctccatt ctgaggccgc ccgccccggc gcgcacggga
     7321 gggagccgcg ttcggtaaca tgctccgcgt gaaggtcttc gcctccatca cgccccacct
     7381 ctccgtcggc gaccgttgcc ggccggtctt cttcgccgga ccctgcgtcc tcgagtcgcg
     7441 cgcgcacgcc ctgaagatgg cgaaggcgat caagcaggtc ttcgccgact gcggcgcggc
     7501 cgaccgcctc gtcttcaagt cctccttcga caaggcgaac cggagcgcgg gagcgagctt
     7561 ccgcggcccg ggaattgaga agggcctgtc gatcctctcc gaggtgaagg agaggaccga
     7621 cctcccgatc ctcacggacg tccacgagcc cgagcagtgc gcgatcgcgg ccgaggtcgc
     7681 ggacgtcctg cagatcccgg ccttcctgtg ccgccagacg gatctcatcg aggccgccgc
     7741 cgcgacgaag aaggccgtga acgtgaagaa aggccagttc ctcgcgccgg acgactgcaa
     7801 gcagatcgtc gacaagtgcc gcgcgaaggg aaacacgaac gtgacgctga ccgagcgcgg
     7861 gacgacgttc ggctaccaca acctcgtcgt ggacttccgc gggctcccga tgatgcgcgc
     7921 gctcggcgtc cccgtgatct acgacgcgac gcactccctt cagctccccg gcggcctcgg
     7981 caaggagacg ggcggctcgc gcgagtacgc cccggccctc gcccgcgccg ccgcggcggt
     8041 cggggtggac ggctacttct tcgaggtcca cgacgacccg gcgcacgcga aatccgacag
     8101 ggccacgcag ctcccgctcg cctcgctgcc ccagttcctg tcggcgcttc tgagcttcga
     8161 ccagctccgg cgggagacga tcgggtaggt ttttcgcctc cggcccttga caggagggct
     8221 tccgcgtccg tactgtgtcg gtgtaatgat acaccgaggc ggtaccccgt gagccgcggc
     8281 ctctccgtcg atcccaccga tgccgttccc ctctggaagc agatcgagga gaacgtgcgg
     8341 cggctcgtcg cctccggggc gctgaagccc ggggcggccg tcccgtccgt gcgcgacctc
     8401 gcccgcgagc tgtcgatcaa tccggccacc gtggcgaagg gctaccagcg tctcgtggat
     8461 gccgggatcc tcgccgtgaa acggggcgag ggcacgttcg tggcggagaa accgccggcg
     8521 atatcgcgcg gcgatcggga caaggagctg gagggcggcg cgctgcgcta cgcgtccctc
     8581 gccgtgacgc tcagcgcgag caacgaggaa gcggtggagc acgtgaaacg cagcatcgga
     8641 tttcttagaa ggaagagaga gggaggggcc gaatgaccac gcagcagcgc ggggacgcgc
     8701 cggtggtgga ggttcggagc ctcctggtgc gctacggcgg caacccgaaa cccacactcg
     8761 aagaaatctc cttctccgtt ccccgcggct ccgtgtatgc cctcctcggg cgcaacggcg
     8821 ccgggaagtc gtctctcgtg cgctgcctcc tcggccagca gaagccgacg gcgggcggct
     8881 gcttcctctt cggcgaggac gtgtggaaac gccgcgcctc gctcatggcg aaagtcggcg
     8941 tcgtccccga ggaccccgac gcgccgccgg cgatgacggc ccggcagctc tccgcgttct
     9001 gcgccaagct ctaccccgtc tgggacgcgc cgtccgtgga ggcgcgcctc gcgcggttcg
     9061 gcgtcccggc cgggacccct tacggaagcc tgtcgaaggg ccagcgcgcg ctcgtctcgc
     9121 tcgcgctcgc cctcgcgtcc tcgcccgacc tgctcgtcct cgacgacccg acgctcggcc
     9181 tcgacgccgt cgcgcggcgc gcgttcttcg aggagctcgt cggcgagctg gcggaccgag
     9241 ggacgaccgt gctcctgacg tctcacgatc tcgcgggcgt cgagagcatg gcgacgcacg
     9301 tcgggttcct gaaggatggg aagctcgccg tggacgagga gctcgagacg ctgaaggggc
     9361 gctttcgtcg cttgcgctat gcgaacgagc agacggagga gcgcacggag tacggccagg
     9421 agctcgcggc cttcgacgcg gtgcgcgtca aggtgcgcgg atggggcata gaggcgatcg
     9481 tctcgaacta cggagaggag tcgttcacgc gcttctcgtc cctcgaaggc gtcgtggacg
     9541 ccgagtcgtc gtcgctgtcg ctggaagaga tcttcgtcgc ggtcgctggc gaaagccccg
     9601 cagggaggaa cgcatgaaag ccttcgccgc cgtcttcgcg cgggaggtct tcgagcgccg
     9661 aatggtcttc ctcgtcgggt tctgctgcgg gatcccgacg ctcgtcgtct cggcccttca
     9721 cggctggaac accccgggta acgccgaggt gcggctcctg gcggcgtcgg tcgggggtct
     9781 cgccctcgcc gtcgcgctcg ccctgctcta cgggggctcg atgatccctg gtgagacggc
     9841 cgaaagacgg atctcgttct ggttctcgcg tccgatcccg gcggcggcga tctgggccgg
     9901 caagctcctc gcggccattt tcgtcagcct ctcggccggc ttcctcgtct ttctcccggg
     9961 ctggctgacc ggacccgctc cgactcgcgc gttatggggc atcgacctgc tcgcgggaca
    10021 ggcggcgctc gcagccctcg cagtcgtggc cggctgcgtc tttgcctccc acgccgtcgt
    10081 cacggtggct cggctgaagt cgccgtgggc cctcctcgac ctgactctcg caatcacgct
    10141 cgcgttcgcc gccaccttcc tcgtccggtc gctggttcgg gaccatctcg gagacccgac
    10201 ctacggcctc gaagcgggtc cggcctggat cggctccgcc atgctcgccg gtgcggtcgc
    10261 gctcgccttc ccgctcgcca cgctcgccca ggtagccgcc ggacgggcgg acgcccgccg
    10321 cgcccacggc gccttctcgt tcgtcctctg ggccgtcctc gggtccggcg tcctcgccgt
    10381 cggcgctttt gcgtggtggt gcgcgtcggc gagggcgacc gacctccgac aggtcgccgg
    10441 aaacgtcgcg tcggctcgac gcggatcctg gaccgccgtc ggcggcgcct tgggaggcgc
    10501 gaggggaggc gcgtcgttcc tcttcgatac ggcgtcgctc gcgtcgatcc gcctccgggg
    10561 ctgcggcgag acgttctcgc agaacggcgc acgcgcagcg tggggcgagc ccgacttcgg
    10621 gatcctcgac cggaggagca accggatgga gctcgtggtg gcggatctcg cctcgggccg
    10681 cgccgtcgcg accggcatcg tgagcgccga ctggccgtgg ctgacgctct cgccgagcgg
    10741 ccacaggctc gcctccctgg cggggttgac gatctccgcc tacgacgtat cggagccggc
    10801 caacccgaag cagctcgcga ctttccgcct cgagcacgcc atgccccgtt tcgccttcgt
    10861 cgacgaggac acgatccgag tcttcccgcg gctctccggc actaccaaac cgaacctcgc
    10921 cgccatcgac gccgagatca gggacctctc cctgtcttcg aagaaatccc tcgtcaccgg
    10981 ccgcctcgac ctcgagtctc tctcctggct gcgtctgagc gcggacgggc gatttctcgt
    11041 cgggacgcgc cgcgcaacag aagacgcctc cggcccccgg accgtcatgc tcctcgacgg
    11101 gcggacgggc gcgcgcgtcg cgacgctcgc ggaggggctc caaagcgcgc aggcgcgcct
    11161 cctcgtcggc aaccggatcg tcgttgccgg catcgccaag gcaggggcac gggcgctttt
    11221 cttcgaaggt gagaggggct ggagcgcgcc cgcccggaca gtcgaactgg gtcccgcggt
    11281 gcgcatcacc ctcggaggcg aaatcgacac ggggcgcgtg gccatttccc tcttcccttt
    11341 cgaagaggaa acaaaggccc acccccgcgc gaagctggtc gtcgtggacg cggcgtcggg
    11401 cgcggtttcc caactcggcg agggcctcgt ccccgtcgac cgcatgggct ggtggatcga
    11461 tcccgtcatg acgcctcccg aagccggcgc gccgtggacg tcgctcttcc tcgacgccga
    11521 cggccgcctc gtccgcctcg accccgccac gggcaagcag acggtgcttc tcggcaaagg
    11581 caagtaaact cgcctggctt gacgacccca gcacggcagc aggccctccg cgtcatcgag
    11641 atcgaggcgg gagccgtccg cgcgctcgcg gacggcctcg acgagggatt cgaccgcgcc
    11701 gtcgccgcca tgaagtccac cgccggccgc gtcgtcgtga cgggcatcgg caagagcggc
    11761 atcgtcggcc agaagatcgc cgcgacgctc gcgtcgacgg gcaccgcggc gttctacctg
    11821 cacccgtccg aggcgatcca cggcgacctc gggatgatcc tcggcggcga cgtcgtcctc
    11881 gccctctcgc actcgggcga gacggaggag gtcgtcgcgc tgatcccgca cgtgaagcgc
    11941 cgcggcgcga cgctggtcgc gatgacggga cggagggact cgaccctcgg aaagggcgcg
    12001 gacgtcgtcg tctcgatcac gatccgcgag gaagcctgcc ccctgaacct cgcgcccacc
    12061 gcctccacga ccgcgcagct cgtcatgggc gacgcgctcg cgatggcgtt gtccgtcgag
    12121 aagggcttcc agcccgagga cttcgccgcg ctccaccccg gcggcaagct cggaaagcgt
    12181 ttcctgaagg tctcggacct catgcacgcg ggcgcggacg tcccgctcgt cgcctccgcg
    12241 gcgccgatga aggacgtcgt ctacgagatg tcccggaagc gcttcggcat cacgggcgtc
    12301 acggaaggcg ggaagctcgt gggcgccgtc tcggacggcg acctccgccg cctcctcgag
    12361 aaggacggcc cgaagatcat gaacgactcg gccgccgacg cgatgggccg gagcccgaag
    12421 acgatcgcga aggacgcctg ggccacggag gcgctgcgga tcatggagga acggaagatc
    12481 acgtccctct tcgtggtggc cccggacggc cgccccgagg gcatcctgca cctgcacgac
    12541 ctgtggggcg tcgagtcgat ttagcgtccc cccgcgcata tgatggcggg agatgcccgc
    12601 ggcgccgatc gttcccgacg acctccggac caagctctcg cgcatccggg ggctcgtcct
    12661 cgacgtggac ggcgtcctga cggacggctc gatgacgttc gacgaggacg gccgggagac
    12721 gaagacgttc gacgtgaagg acgggctcgg cctcgtcctc ctgcgcgacc tcgggtacag
    12781 gatcggcgtc atctcgtccc ggacgtcccg ggtcgtctcg cgccggtgcg ccgacctgaa
    12841 gatccccgag gaattcgtcc tccagggcga gcgcgacaag gccgccgcct tcgacctcct
    12901 cgcgaagcgc tgggacctcg aggcgtggga gatcgcggcc gtcggggacg acctgcccga
    12961 cctcgggatg ctccgccgcg cgggcgtggg cgcc
//
LOCUS       DAOMFY010000091        12893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1492_length_12893_cov_8.582178, whole genome shotgun sequence.
ACCESSION   DAOMFY010000091 DAOMFY010000000
VERSION     DAOMFY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12893
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1492_length_12893_cov_8.582178"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLB01_09260"
     CDS             complement(<1..880)
                     /locus_tag="PLB01_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ77529.1"
                     /translation="MTRPVPRTACAAAALTLLFAGLTPIARAQNADFVWYGGPILAGK
                     DARPEALAVKDGKIVALGSRADVEKKWKGKSTLPIDLNGQTLVAGLVPTPMPTPPPAA
                     AKKGSAKIEKPPRATPTPAPKPTPTPPPPSEPAPPLEALGADVSPPPAGLPYTIASVR
                     DALRMWILRSSLPKRHRVVVGFGYDETKLAESRPPTREELDTVSATLPVIVVSRAGDA
                     GAYNTLALQKAGISWRSKDPPGGTIVRRGRIQEPNGVLVGAAHAAAFAKLVPRLDEKE
                     RARLHAESVRLNTPEEP"
     gene            932..3184
                     /locus_tag="PLB01_09265"
     CDS             932..3184
                     /locus_tag="PLB01_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77530.1"
                     /translation="MTLFRSGEELILPELAQAQGLASNTFTVVDVIRGGMGVCAKVRQ
                     GQGPAFAVKALPAGEKKGEAFERFLVELQTWLKLSVCDGIVEALCVFTYKDTPVVCSR
                     WMNGGNLRPYLAERDPEVFYRTIVRIAGALEWAYREHGILHLDLKPENILIDDLHRAA
                     VSDWGIAGTRGEIDPAATDGAMVIYGTAAYASPEQLLGGVPLDVRSDIYSLCCLMYEW
                     ETGRVPFTGDWHEIRGAKIRNEPPRLASGMFRRSVFGADDIIMRGLERERHGRYPDWK
                     AFATDVLNASLRRELKIGRFIPKMRYQASEVRSDVLRAKVATGAMVTGRGGSTVDVRG
                     AKKELDEALRFAEKEDWTSAVEILSRVVLPSVVREHPDDPLQQTSAITLARCLLKLDR
                     PAEAVAALDALSAATDKPSDMFALLADAHLRLGNASLAERAARTGLLLHKTDATLLDL
                     LLAAQGAQGLSSDAVATARQRLAGRRDAGTLLDAAAQLLQHAATVPETRLPEQLAEFH
                     EALALAAEARNLAPKDHRPRVIRARALQGLDRWAEAKAELAGFPEGGDAALRRAAAET
                     EARGLLRLKEYAGCLALCNKALAAHPESASLARTRALAFAEGFILGVETGGRRVVDDA
                     AMGFFERAVTDPAVREPGDFVTLARYREWRGRAEAAVSVLRDGKDAFPKSWEIGVALA
                     RLLERRGDFEESLEAALETTRNAPFRPEAWRALAAVKGVLGPKAEADEARQKGAQAEK
                     RLKELRAKSA"
     gene            complement(3192..3896)
                     /locus_tag="PLB01_09270"
     CDS             complement(3192..3896)
                     /locus_tag="PLB01_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQQ77531.1"
                     /translation="MKREYGSWRSSALARPMEYLWFGDRGRPVLMFPTSMGRFYQNED
                     FGLTGALADKVDAGWLQLVCVDSVDEESWYNKKIHPRDRARRHDDYDRYLRDEMLPYI
                     ESRAGGNVVTVGASFGAYHAANLAGRYPGRVTKAICFSGLYDVSRYLDGYWDDLCYYH
                     TPNAYIANMDEGWKEKLRAVDWVVATGEQDSLIRDNRDFAALLARQGQRVTAEFWPGV
                     FGHDWPFWNEHIRRFI"
     gene            3978..4931
                     /locus_tag="PLB01_09275"
     CDS             3978..4931
                     /locus_tag="PLB01_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77532.1"
                     /translation="MARKRIGVLFGMEDTFPWALMNEINEMAAKNGDDVEAGPVKVSV
                     LVQEQSFEDAVILDRISHEVPFYRTFLKCAVARGAQVVNNPIWWCADDKYFDNIVALA
                     AGVAVPRTVLLPHKEHPPNTTATSFRNLDFVDWDRVFAYLGFPIFMKPANGGGWKDVY
                     KCDTPEEFFAAYDKTRDLEMMAQEAIEFTEYYRCYVVGRSRVKIMPYAPKEPHAMRYA
                     AVAGKPVPAAMAERVTNDALALCNALGYDLNTVEFAVRDGVPYAIDFMNCAPDADRAS
                     VGEENFRWIVKAMAEYLVDRAKNPRPFEATGTWPALLGLVK"
     gene            4948..6060
                     /locus_tag="PLB01_09280"
     CDS             4948..6060
                     /locus_tag="PLB01_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HQQ77533.1"
                     /translation="MGAPRPSFTLGIEEEFQIVDPRTRALKAHIQELFAEGESRLKDK
                     IKREFHQSVIEVGTGICRNIAEARADVTNLRQELVRLTGEAGLAIGAAGTHPFTHWSE
                     VPVTPNPRYERILYDLGTVARGNLIFGLHVHVAVEHDETRIALQNAARYFLPHVFALS
                     TNSPFWGGRETGWKSYRAKVFDRFPRTGIPDQFVSWADYMSYVETLVKTNCIDNPKKI
                     WWDVRPHPFFPTLEYRICDIPMRVDETICLAALFQAITVKLWKLHDRNLSYRLYSRAL
                     ISENKYRAARFGIDGALIDFGKKEEVPFTDLLGELLEFVDDVLDELGSRKEVEYVHTI
                     VKRRTGADRQLEVFERTKSLPDVVDYILEETRHGLD"
     gene            6083..7501
                     /gene="murF"
                     /locus_tag="PLB01_09285"
     CDS             6083..7501
                     /gene="murF"
                     /locus_tag="PLB01_09285"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQQ77534.1"
                     /translation="MTGALGVAAPAGAPAAFPSVSTDTRAIPAGALFVALKGERFDAH
                     AFLAEAKAKGAGAAVVRKGTPAVPGLPFFEVADTLGALGALAAFHRRRFEIPVAAVAG
                     SNGKTTTKEMLASILRTRGPALATEGNLNNEVGVPLTVFRLAREHRAAVLELGMSAPG
                     ELARLTEIARPDAGVVTLVAAEHLEFLKDLDGVADAEGELYRGLRPGSFAVVNADDPR
                     CVAQAERAAVGVKKIFFGRSPLADVRLVESTSLGVEGQKICLEGEEWEGVAGAGRLVG
                     GAKRQRRSVEVTLGFVGQHNAMNAAAAAATARALSYSFEEISRGLSLAKPYSHRSRVL
                     SAPGGVTILDDCYNASPASMEAALTTLASLVEGGKGRAVAVLGDMLELGAFEEEAHRA
                     LGRSARDARVAVAAFFGPRSPLTFKEFFSSSSSSAHFTEIEPLLVWLRPRLAPGDVVL
                     VKGSRGMKLERVVDALAAGAGA"
     gene            complement(7503..8033)
                     /locus_tag="PLB01_09290"
     CDS             complement(7503..8033)
                     /locus_tag="PLB01_09290"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HQQ77535.1"
                     /translation="MKRTTFALALLFGTGLAAAAGIPEKLDAPNYKRLSPSLAVSGKP
                     SKEALASLREAGFRTAVDLRQPTEGVSSAREAVEAQGLKFVSVPVSPNTFSLDDVKKV
                     EAVLKDQAAAPVFLFCSSSNRVGGAIAVIEYLRGRSKDEAIAEGKKAGLRSPEMEKAA
                     LRVMDQEPAAAARGVK"
     gene            complement(8046..9185)
                     /locus_tag="PLB01_09295"
     CDS             complement(8046..9185)
                     /locus_tag="PLB01_09295"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQQ77536.1"
                     /translation="MRVVPPGSTVGVLGSGQLGRMFALSARAMGYRVHTYSPDEDSPT
                     GHVADVEVSAPYDDLDALRAFAKGLDVLTFEFENVPRAALDALESLVPVRPGADALFV
                     SQNREREKEFLGRVNVPVAPWAVVRTSEDLASALSSVGAPAVLKTAAFGYDGKGQARV
                     HSKNLPDAEAAWAALGRVPCVLESFVDFQREVSVIAARGADGSVAFFPIFENAHKQHI
                     LDVTTAPAPLTLSEETLAHEIVQAILEALGFVGVLCVEMFHAADGRLLVNEIAPRPHN
                     SGHLTIDACVTSQFEQQLRAVAGLPLGSTELLRPAAMANLLGDLWAGGPPRWENACAA
                     PGVALHLYGKKEPRPGRKMGHLTALAATPAEAWRTVLEARASLLR"
     gene            complement(9182..9676)
                     /gene="purE"
                     /locus_tag="PLB01_09300"
     CDS             complement(9182..9676)
                     /gene="purE"
                     /locus_tag="PLB01_09300"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQQ77537.1"
                     /translation="MKKVARPLVGVVMGSTSDWETMRWAYETLERFGVPCEKRVVSAH
                     RTPKLLASYASEAEERGLEVIIAGAGGAAHLPGMLAAQTSVPVLGVPVESRALKGLDS
                     LLSIAQMPAGVPVGTLAIGVPGSINAGLLAVAIVANAHPEFRPRLKGFRAKKTREVVK
                     SRLP"
     gene            complement(9698..10555)
                     /locus_tag="PLB01_09305"
     CDS             complement(9698..10555)
                     /locus_tag="PLB01_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HQQ77538.1"
                     /translation="MNVLVLGGTGTVGSQVVRELLKRGADVSVLTRDAKKPLAAGAKP
                     VAGDLLDPSTIRSAFAGRDAVFLLTALGAGECHEGLMAVNGCRLGGVKKVVFLSVHRV
                     DEAPHLPHFGAKLPIETAIRDSGIPWTILRPNNFFQNDFWYKDAMLSYGVYPQPFGDA
                     GLSRVDVRDIAEVAALALTTDAAKNETVNLVGPEILTATGTAATWGGVLGRKIAYGGN
                     DLDAWEKATLAFMPAFAVFDFRLMYAFFQEKGLKAVPEDVARCAALLGHPPRRFDEFA
                     AETARAWSA"
     gene            complement(10569..11867)
                     /gene="icd"
                     /locus_tag="PLB01_09310"
     CDS             complement(10569..11867)
                     /gene="icd"
                     /locus_tag="PLB01_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQQ77539.1"
                     /translation="MPSAKPTVPEGGQKITISGGKLQVPDHPVIPFIEGDGTGPDIWR
                     SSVRVLDAAVAKAYGGKRQIKWMEVYAGQKSFDHLQTWLPDETIDAFREFLVGIKGPL
                     TTPIGGGIRSLNVALRQLLDLYVCLRPVRWFQGVPSPVKHPEKVNMVIFRENTEDIYA
                     GIEFAAGSDEAKKFAEFLKTNFPAQYKKVRFPETTGFGVKPVSQEGSERLIRAAIEYA
                     IKNKSKSVTFVHKGNIMKFTEGAFRNYGYALAEREFAGQTYTWDQWERTKKDKGEAAA
                     NAEQKAELAKGKILVKDAIADITLQQVLTRPDEFDVIATLNLNGDYLSDALAAQVGGI
                     GIAPGGNVNYVTGHAVFEATHGTAPKYADLDKVNPGSVVLSGEMMLRYMGWTEAADLI
                     LKGMDGAIRARTVTYDFARQMEGATEVSCSKFGDAVISHM"
     gene            11965..>12893
                     /locus_tag="PLB01_09315"
     CDS             11965..>12893
                     /locus_tag="PLB01_09315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ77540.1"
                     /translation="MAGPPAPRTYSVVIPAFNAERFLGEAIASALAQTLPPLEVLVVD
                     DGSTDGSRAVAEGFGAAVRVFTGPNAGPSAARNRGVREARGSWIAFLDADDLFEPGYL
                     AAAEEHLAAHPGVGLLCSGVRILEGGRPTSHVILKKTPGPAYTTMGLLEGDIGTICTP
                     LVARDVFLASGGFDESFRANEDGHLWLRLSRVTGVHQTREPLLLYRRHADNASGDLLA
                     NARDSVRSLERLEETHPEFARELRRPMHRLRGKEYLRLGRELLVRGEDLPGARAALRK
                     AVTFRPGRLRGWLYLAFASVPGGPGAVAAFRRR"
BASE COUNT         1921 a         4410 c         4599 g         1963 t
ORIGIN      
        1 ccggctcttc gggcgtgttc agccgcacgc tctccgcgtg gaggcgcgcg cgctccttct
       61 cgtcgagacg cgggacgagc ttcgcgaacg ccgcggcgtg cgcggcgccg acgaggacgc
      121 cgttcggctc ctggatgcgc ccgcggcgga cgatcgtgcc gcccggaggg tccttcgagc
      181 gccacgaaat cccggccttc tgcagggcga gcgtgttgta cgcgccggcg tcgccggcgc
      241 gcgacacgac gatgacgggc agcgtcgccg agaccgtgtc gagctcctcg cgcgtgggcg
      301 ggcggctctc cgcgagcttc gtctcgtcgt aaccgaagcc gacgacgacg cggtgccgct
      361 tcgggaggct cgagcgaagg atccacatcc ggagcgcgtc gcggacggac gcgatcgtgt
      421 aggggaggcc ggccggcggc ggcgacacgt cggctcccag cgcctcgagc ggcggagcgg
      481 gctcgcttgg cggcggaggc gtcggcgtcg gcttcggggc cggcgtcggc gtcgcccggg
      541 gcggcttctc gatcttcgcg gagcctttct tcgccgccgc gggaggcggc gtcggcatgg
      601 gagtcggcac gaggcccgcg acgagggtct ggccgttcag gtcgatcggg agggtggact
      661 ttcccttcca cttcttctcg acgtcggccc gcgaaccgag cgccacgatc ttcccgtcct
      721 tcacggcgag ggcttccgga cgggcgtcct ttcccgcgag gatcggcccg ccgtaccaga
      781 cgaagtccgc gttctgggcc cgggcgatcg gagtgaggcc ggcgaagagc aaggtgaggg
      841 cggcggcggc gcaggcggtc cgtgggacgg ggcgcgtcat cggagcgccg atcgtaccgc
      901 cggccggcgc tggtgagaga attgggtaca ggtgactttg tttcgttcgg gcgaagagct
      961 gatcctgccc gagctggcgc aggcgcaggg gctcgcctcc aacacgttca cggtcgttga
     1021 cgtcatccgc ggcgggatgg gcgtctgcgc gaaggtccgg cagggccagg gccccgcgtt
     1081 cgccgtgaag gcgctgcctg cgggcgagaa gaagggcgag gccttcgagc gctttctcgt
     1141 cgagctccag acctggttga agctgtcggt ctgcgacgga atcgtcgagg cgctctgcgt
     1201 cttcacgtac aaggacacgc cggtcgtgtg ctcgcgctgg atgaacggcg gaaacctccg
     1261 gccgtacctc gcggaaaggg acccggaggt cttctaccgc acgatcgtcc gcatcgcggg
     1321 cgcgctcgag tgggcctatc gcgagcacgg gatcctccac ctcgacctga agcccgagaa
     1381 catcctgatc gacgacctgc accgcgcggc cgtgtcggac tggggaatcg cgggaacccg
     1441 cggcgagatc gacccggccg ccacggacgg ggcgatggtc atctacggca ccgcggcgta
     1501 cgcatccccc gagcagctcc tcggcggcgt gccgctcgac gtgcggtcgg acatctacag
     1561 cctctgctgc ctcatgtacg agtgggagac ggggcgcgtc cccttcacgg gggactggca
     1621 cgagatccgc ggcgcgaaga tccgcaacga gcctccgcgc ctcgcgtccg ggatgttccg
     1681 ccgctccgtg ttcggcgcgg acgacatcat catgcgcggc ctcgagcgcg agcggcacgg
     1741 ccggtacccg gactggaagg cgttcgccac cgacgtcctg aacgcgtcgc tccggcgcga
     1801 gctcaagatc ggccggttca tcccgaagat gcgctaccag gcgtccgagg tgcgcagcga
     1861 cgtgctgcgc gccaaggtcg cgaccggcgc gatggtgacg ggccgcggcg ggtcgacggt
     1921 ggacgtccgc ggcgccaaga aggagctgga cgaggcgctg cgcttcgcgg agaaggagga
     1981 ctggacctcc gcggtcgaga tcctctcgcg cgtcgtcctt ccgtcggtcg tgcgggagca
     2041 cccggacgac ccgctccagc agacctcggc gatcacgctg gcgcggtgcc tcctcaagct
     2101 cgaccgccct gccgaggccg tcgccgcgct cgacgccctc tccgcggcga cggacaagcc
     2161 gtccgacatg ttcgcgctgc tcgcggacgc gcacctgcgt ctcggcaacg cgagcctcgc
     2221 cgagcgcgcc gcgcgcaccg ggctcctcct gcacaagacc gacgccaccc tcctcgacct
     2281 tcttctcgcc gcgcagggcg cccagggcct ctcgtcggac gcggtcgcga ccgcgcgcca
     2341 gcggctcgcg ggacggcgcg acgccggaac gctcctggac gcggccgcgc agctcctcca
     2401 gcacgccgcg accgtcccgg aaacccgtct cccggagcag ctcgcggagt tccacgaggc
     2461 gctcgcgctc gccgcggagg cccggaacct cgcgccgaag gaccaccggc cgcgggtgat
     2521 ccgagcgcgc gccctgcagg gcctcgaccg ctgggccgag gcgaaggcgg agctcgccgg
     2581 gttccccgag ggcggcgacg cggcgctgag gcgcgcggcc gccgagaccg aggcgcgggg
     2641 cctcctgcgc ctcaaggagt acgcgggctg cctcgccctg tgcaacaagg cgctcgcggc
     2701 gcacccggag agcgcgagcc tcgcgcgaac gcgcgcgctc gccttcgcgg agggtttcat
     2761 cctgggcgtc gagacgggcg gacgccgcgt cgtcgacgac gcggcgatgg ggttcttcga
     2821 gcgggcggtc acggacccgg cggtgcgcga gccgggcgac ttcgtgacgc tcgcgcggta
     2881 ccgggagtgg agggggcgcg ccgaggccgc cgtctcggtg ttgagggacg ggaaggacgc
     2941 gtttccaaaa tcctgggaga tcggcgtcgc gctcgcgcgc ctgctcgaga gacgcggcga
     3001 cttcgaggaa tccctcgagg cggcactgga gacgacgcgc aacgccccgt tccggccgga
     3061 ggcctggcgc gctctcgcgg cggtcaaggg cgtcctcggg ccgaaggccg aggcggacga
     3121 ggcgcggcag aagggcgccc aggccgagaa acgcctcaag gagctgcgcg cgaagtccgc
     3181 ctgaccgccg gtcagatgaa ccggcggatg tgctcgttcc agaacggcca gtcgtgcccg
     3241 aagacgccgg gccagaactc cgccgtcacg cgctgcccct ggcgggcgag gagcgccgcg
     3301 aaatcgcggt tgtcgcggat cagggagtcc tgctcgcccg tcgcgacgac ccagtccacg
     3361 gcccgcagct tctccttcca cccctcgtcc atgttcgcga tgtaggcgtt gggcgtgtgg
     3421 tagtagcaga ggtcgtccca gtagccgtcg agatagcgcg acacgtcgta gagaccggag
     3481 aagcagatcg ccttcgtcac gcgccccggg taccggcccg caaggttcgc cgcgtggtac
     3541 gcgccgaagc tcgcgccgac cgtcacgacg ttgccgccgg cgcggctctc gatgtagggg
     3601 agcatctcgt cgcgcaggta gcggtcgtag tcgtcgtggc gccgggcgcg gtcgcgcggg
     3661 tggatcttct tgttgtacca gctctcctcg tccaccgaat ccacgcagac gagctgcagc
     3721 cagccggcgt cgaccttgtc cgcgagcgcg cccgtgaggc cgaagtcctc gttctggtag
     3781 aagcgcccca tcgacgtcgg gaacatgagg acggggcgtc cgcggtcccc gaaccagagg
     3841 tattccatcg ggcgcgcgag cgcggagctt ctccacgagc cgtattcgcg tttcatgggc
     3901 gcggagtcta gcgcggtgcg tttgcggcct ctcgcgcgcc tccgctagga tgcgtcgcgc
     3961 gacgcgaggg ggcactcatg gcacgtaaga ggatcggcgt cctgttcggg atggaagaca
     4021 ccttcccgtg ggcgctcatg aacgagatca acgagatggc cgcgaagaac ggcgacgacg
     4081 tggaggcggg gcccgtgaag gtctcggtcc tcgtccagga gcagtccttc gaggacgccg
     4141 tgatcctcga ccgcatcagc cacgaggtcc cgttctaccg gacgttcctc aagtgcgcgg
     4201 tcgcgcgcgg ggcccaggtc gtgaacaacc cgatctggtg gtgcgccgac gacaagtact
     4261 tcgacaacat cgtcgcgctc gcggcgggcg tcgccgttcc caggaccgtt ctccttcccc
     4321 acaaggagca cccgccgaac acgaccgcga cgtccttccg gaacctcgac ttcgtcgact
     4381 gggaccgcgt gttcgcgtac ctcggcttcc cgatcttcat gaaaccggcg aacgggggcg
     4441 gatggaagga cgtctacaag tgcgacacgc cggaggagtt cttcgcggcg tacgacaaga
     4501 cgcgggacct cgagatgatg gcgcaggaag ccatcgagtt cacggagtac taccgctgct
     4561 acgtcgtcgg gcgctcgcgc gtgaagatca tgccgtacgc gccgaaggag ccgcacgcca
     4621 tgcgttacgc cgccgtggcc gggaaaccgg tccccgcggc catggccgag cgcgtgacga
     4681 acgacgcgct cgccctctgc aacgccctcg gatacgacct caacacggtg gagttcgccg
     4741 tgcgcgacgg ggtcccctac gcgatcgact tcatgaactg cgcgcccgac gcggaccgcg
     4801 cgtcggtcgg cgaggagaat ttccggtgga tcgtcaaggc gatggcggaa tacctcgtcg
     4861 atcgcgcgaa gaatccccgg ccgttcgagg cgacgggcac gtggccggcc ctcctcggcc
     4921 tcgtgaagtg agacggggat ccccgccgtg ggcgctcccc gcccgtcctt caccctcggg
     4981 atcgaggagg agttccagat cgtcgacccc cggacgcgcg cgctgaaggc gcacatccag
     5041 gagctcttcg ccgagggcga gtcgcgcctg aaggacaaga tcaagcgtga gttccaccag
     5101 tcggtcatcg aggtcgggac cggaatctgc cgcaacatcg ccgaggcgcg cgcggacgtc
     5161 acgaacctga ggcaggaact cgtccgcctg acgggcgagg cgggccttgc gatcggcgcg
     5221 gccgggacgc acccgttcac gcactggtcc gaggtccccg tgacgccgaa ccctcgctac
     5281 gagcgcatcc tttacgacct cggcacggtg gcgcgcggca acctcatctt cggcctccac
     5341 gtccacgtgg cggtcgagca cgacgagacc cgcatcgcac tccagaacgc ggcgcgctat
     5401 ttcctgccgc acgtcttcgc actctcgacg aactcgccct tctggggtgg gcgtgaaacc
     5461 ggctggaaga gctaccgcgc gaaggtgttc gaccggttcc cgcgcacggg catcccggac
     5521 cagttcgttt cgtgggccga ctacatgagc tacgtcgaga cgctcgtgaa gacgaactgc
     5581 atcgacaacc cgaagaagat ctggtgggac gtgcgcccgc acccgttctt cccgacactc
     5641 gagtaccgga tctgcgacat cccgatgcgc gtggacgaga cgatctgcct cgcggctctc
     5701 ttccaggcca tcaccgtcaa gctctggaag ctccacgacc ggaatctctc gtaccgcctc
     5761 tactcgcggg cgctcatcag cgagaacaag taccgggcgg cccgcttcgg gatcgacgga
     5821 gcgctcatcg acttcggaaa gaaggaagag gtgccgttca cggacctcct cggcgagctc
     5881 ctggagttcg tggacgacgt cctcgacgag ctcggctcgc ggaaggaagt cgagtacgtc
     5941 cacacgatcg tgaagcgcag gacgggcgcg gaccgccagc tcgaggtctt cgagagaacg
     6001 aagagcctcc cggacgtcgt cgactacatc ctcgaggaga cgcgccacgg cctcgactga
     6061 gccgctgttc tcggccgcgg acctgacggg tgcgctcggc gtcgccgcgc ccgcgggcgc
     6121 gccggcggcc ttcccgtccg tctcgacgga cacgcgcgcg atcccggcgg gcgcgctgtt
     6181 cgtcgcgctg aaaggggagc ggttcgacgc gcacgcgttc ctcgcggagg cgaaggcgaa
     6241 gggcgccggc gccgccgtcg tccggaaggg gacgcccgcc gtgccgggac tcccgttctt
     6301 cgaggtcgcg gacacgctcg gcgcactggg cgcgctcgcc gccttccacc gccggcggtt
     6361 cgagatcccg gtcgcggccg tggcgggttc caacggcaag acgacgacga aggaaatgct
     6421 cgcgtcgatc ctccggacgc gggggcccgc gctcgcgacc gagggcaacc tcaacaacga
     6481 ggtcggagtt ccgctgacgg tcttccggct cgcgagagag catcgcgccg ccgtcctcga
     6541 gctcgggatg tcggcgccgg gcgagctcgc ccggctcacg gagatcgccc ggcccgacgc
     6601 gggcgtcgtc accctcgtcg ccgccgagca cctggaattc ctgaaggacc tcgacggcgt
     6661 cgcggacgcg gaaggcgagc tctaccgggg cctccggccg ggctccttcg ccgtcgtgaa
     6721 cgcggacgac ccgcgctgcg tggcgcaggc ggaacgcgcc gccgtcggcg tgaagaagat
     6781 cttcttcggc cggagtcccc tggcggacgt ccggctcgtc gagtcgacgt ctctcggagt
     6841 ggagggtcag aagatttgct tagaaggtga agaatgggag ggggtcgctg gagcaggccg
     6901 cctcgtcggc ggcgccaaac gacagcggcg ctcggtcgag gtcacgctgg ggttcgtggg
     6961 gcagcacaac gcgatgaacg cggcagcggc ggcggccacg gcacgggccc tctcctactc
     7021 attcgaagaa atctcccgcg gcctgtccct cgcgaagccc tactcgcacc ggtcgcgcgt
     7081 cctctctgct cccgggggcg tcacgatcct cgacgactgc tacaacgcct cgcctgcttc
     7141 catggaggcc gcgctcacga cgctggcgtc gctcgtcgag ggagggaaag ggcgggcggt
     7201 cgcggtgctc ggcgacatgc tggagctggg agccttcgaa gaagaggccc acagggctct
     7261 gggacgcagc gcgcgcgatg ctcgcgtcgc cgtggccgcg ttcttcggcc cccgttcccc
     7321 tcttaccttc aaagaattct tctcgtcatc ttcctcttcc gcgcacttca ccgagatcga
     7381 gccgctgctc gtgtggctcc gcccgcggct ggcgccgggg gacgtcgtcc tcgtgaaggg
     7441 gagccggggg atgaagctcg aacgcgtcgt ggacgcgctc gccgccggcg cgggggcctg
     7501 agctacttca cgcccctcgc ggccgccgcg ggctcctggt ccatcacgcg cagggcggcc
     7561 ttctccatct cggggctgcg caggccggcc ttctttccct ccgcgatcgc ctcgtccttc
     7621 gaacgtcccc gcaggtattc aatgacggcg attgcgccgc cgacgcggtt gctcgacgag
     7681 cagaagagga agacgggcgc agcggcctgg tccttcagga ccgcctcgac cttcttcacg
     7741 tcgtcgaggg agaaggtatt cggcgagacg ggcaccgaga cgaacttcag cccctgcgcc
     7801 tcgacggcct cgcgagccga ggacacgcct tcggtcggct ggcgcaggtc gacggccgtc
     7861 cggaagccgg cctccctgag cgatgcaaga gcctctttcg agggcttgcc ggagacagcg
     7921 aggctcggcg acaggcgctt gtagttcggc gcgtcgagct tctcggggat gccggcggcg
     7981 gccgcgaggc cggtgccgaa gaggagggcg agggcgaacg tcgtgcgctt catgagacgg
     8041 ggaatctagc gcaggagaga ggcgcgcgcc tcgaggacgg tccgccacgc ctctgcgggc
     8101 gtcgccgcga gggccgtcag gtggcccatc ttgcggccgg ggcgcggctc cttcttgccg
     8161 tagaggtgaa gcgcgacgcc cggcgcggcg caggcgttct cccagcgcgg ggggccgccc
     8221 gcccagaggt cgccgagcag attcgccatc gcggcggggc ggagcagctc cgtcgagccg
     8281 agcggcaggc cggcgacggc gcgcagctgc tgctcgaact ggctcgtgac gcaggcgtcg
     8341 atcgtgaggt gcccggagtt gtgcgggcgc ggggcgatct cgttgacgag gaggcggccg
     8401 tccgccgcgt ggaacatctc aacgcagagg acgccgacga aaccgagcgc ttcgaggatt
     8461 gcctgtacga tttcgtgagc aagagtttct tctgaaaggg taagaggggc aggggcggtc
     8521 gtcacgtcca ggatgtgctg cttgtgcgcg ttctcgaaga tcggaaagaa cgcgacactt
     8581 ccgtcggcgc cgcgcgcggc gatgacggag acctcgcgct gaaagtcgac gaaagattcg
     8641 aggacgcagg gcacgcggcc gagggcggcc catgccgcct ccgcatccgg gagattcttc
     8701 gaatgaaccc tcgcctggcc cttgccgtcg tacccgaacg ccgccgtctt gaggacggcg
     8761 ggggcgccga cggaggagag agccgaggca aggtcctcgc tcgtgcgcac caccgcccac
     8821 ggtgcgacgg ggacattcac ccttccaaga aattctttct ccctctctct attctgagaa
     8881 acaaaaagag cgtccgcccc gggtcgaacg ggaacgagag attcgagcgc gtcgagcgcc
     8941 gcgcgcggga cgttctcgaa ctcgaacgtg aggacgtcga ggcccttggc gaacgcgcgg
     9001 agcgcgtcga ggtcgtcgta cggagccgag acctcgacgt cggcgacgtg gccggtcgga
     9061 gagtcctcgt ccggcgaata cgtgtggacg cggtagccca tggcgcgcgc ggagagcgcg
     9121 aacatgcggc cgagctggcc gctcccgagg acgccgaccg tggagccggg cgggacgact
     9181 ctcacggcag ccgggatttg acgacttcgc gcgtcttctt cgcgcggaag cccttgagcc
     9241 gggggcggaa ctccgggtgt gcgttcgcca cgatcgcgac ggcgaggagg ccggcgttga
     9301 tcgatccggg gacgccgatc gcgagcgttc cgacggggac gccggcgggc atctgcgcga
     9361 tcgagaggag ggagtcgagg cccttgagcg cccggctctc gacggggacg ccgaggacgg
     9421 gcacggacgt ctgcgccgcg agcatcccgg gcagatgcgc ggcgccgccc gcgccggcga
     9481 tgatgacctc gagtccgcgc tcctcggcct ccgacgcgta cgaggcgagg agcttcggcg
     9541 tccggtgcgc cgagacgacc cgcttctcac acgggacgcc gaatcgttcg agcgtctcgt
     9601 acgcccagcg catcgtctcc cagtcggacg tggaacccat gacgaccccg acgaggggcc
     9661 gtgcgacctt cttcattccg gggatcctag cccgaactca ggcgctccag gcgcgggcgg
     9721 tctcggcggc gaactcgtcg aagcgccgcg gcggatggcc gagaagggcc gcgcacctcg
     9781 cgacgtcctc cggcacggcc ttgaggccct tctcctggaa gaacgcgtac atgagccgga
     9841 agtcgaacac cgcgaatgcg ggcatgaagg cgagggtcgc cttctcccag gcgtcgaggt
     9901 cgttcccgcc gtacgcgatc ttgcgcccga ggacgccgcc ccacgtcgcg gccgtgcccg
     9961 tcgcggtcag gatctccggg cccacgagat tgaccgtctc gttcttcgcc gcgtccgtcg
    10021 tgagggcgag cgcggcaact tcggcgatgt cgcgcacgtc gacgcgcgag aggccggcgt
    10081 cgccgaacgg ctgcgggtag acgccgtagg agagcatggc gtccttgtac cagaagtcgt
    10141 tctggaaaaa gttgttcggc ctcaggatcg tccaggggat gccggaatcc cgaattgccg
    10201 tctcgatggg aagtttggcc ccgaagtggg ggagatgggg cgcctcgtcc acgcggtgga
    10261 ccgacaggaa gacgaccttc ttcacgccgc cgagccggca gccgttcacg gccatgaggc
    10321 cttcgtggca ctcgcctgcg cccagggccg tgaggaggaa gacggcatcg cggcccgcaa
    10381 aagccgagcg gatggtggac ggatcgagga gatcgcccgc gaccggcttt gcgccggccg
    10441 cgagcggctt tttcgcgtcg cgggtcagta cggagacgtc cgcacccctc ttgagaagct
    10501 cccggacgac ctgggatccg acggttccgg tgcctccgag gacgagtacg ttcacgggcg
    10561 cctcctcctt acatgtggga aatgaccgcg tctccgaact tgctgcagga aacctcggtg
    10621 gcgccttcca tctggcgcgc gaagtcgtac gtgaccgtcc gggcccggat cgcgccgtcc
    10681 atccccttca ggatgaggtc ggccgcttcg gtccagccca tgtaccgcag catcatctca
    10741 ccggagagga cgacggagcc cgggttgacc ttgtcgaggt ccgcgtactt cggcgccgtg
    10801 ccgtgcgtcg cctcgaagac ggcgtggccc gtcacgtagt tcacgttgcc gcccggcgcg
    10861 atgccgatgc cgccgacctg cgcggcgagg gcgtccgaga ggtagtcgcc gttgaggttc
    10921 agcgtcgcga tgacgtcgaa ctcgtcggga cgcgtcagaa cctgctggag cgtgatgtcc
    10981 gcgatggcgt ccttgacgag gatcttgccc ttcgcgagct cggccttctg ctcggcgttc
    11041 gcggcggcct cgcccttgtc cttcttcgtg cgctcccact ggtcccacgt gtacgtctgg
    11101 ccggcgaact ccctctccgc gagggcgtag ccgtagttcc ggaacgcgcc ttccgtgaac
    11161 ttcatgatgt tgcccttgtg gacgaacgtg acgctcttcg acttgttctt gatcgcgtac
    11221 tcgatggcgg cccggatgag gcgctcgctg ccttcctgcg agacgggctt cacgccgaag
    11281 ccggtcgtct ccgggaagcg gaccttcttg tactgcgccg ggaagttcgt cttgaggaac
    11341 tcggcgaact tcttcgcctc gtcggagccc gccgcgaact cgatgccggc gtagatgtcc
    11401 tcggtgttct cccggaagat gaccatgttc accttctcgg ggtgcttcac gggggagggg
    11461 acgccctgga accagcgcac gggacggagg cagacgtaga ggtcgagaag ctggcggagc
    11521 gcgacgttca gcgagcggat cccgccgccg atcggcgtcg tcaggggccc cttgatgccg
    11581 acgaggaact cgcggaacgc gtcgatcgtc tcgtccggga gccaggtctg gaggtggtcg
    11641 aaggacttct gccccgcata gacctccatc cacttgatct gcctcttgcc gccgtaggcc
    11701 ttggcgaccg cggcgtcgag cacgcgcacc gaggagcgcc agatgtccgg gccggtcccg
    11761 tcgccttcga tgaaggggat gaccggatgg tcgggaacct ggagtttccc gccggaaatc
    11821 gttatcttct ggccgccttc ggggaccgtg ggcttggcgc tgggcatgag aatcctcctt
    11881 cgttcgggcg cggatcatag gggccgggca agggtcccgg caacgaagcg aatgaatcag
    11941 gcgtgggcgc ttagactggc gcccatggcc ggcccgcccg ccccgcgcac gtacagcgtc
    12001 gtgatcccgg ccttcaatgc cgaacgcttt ctcggcgagg cgatcgcgag cgcgctcgcg
    12061 cagacgctcc cccctctcga ggtcctggtc gtcgacgacg gatccacgga cggaagccgg
    12121 gcggtcgccg aggggttcgg ggcggccgtc cgcgtcttca cgggcccgaa cgccggcccg
    12181 tcggccgccc ggaaccgcgg ggtccgcgag gcgcgcgggt cctggatcgc cttcctcgat
    12241 gcggacgacc ttttcgagcc gggctacctc gcggcggccg aggagcacct cgcggcgcac
    12301 ccgggcgtcg gcctcctctg ctccggcgtg cgcatcctcg agggcgggcg tccgacgtcg
    12361 cacgtcatcc tcaagaagac gccggggccc gcgtacacga ccatggggct cctggagggc
    12421 gacatcggga cgatctgcac gcccctcgtc gcgcgcgacg ttttcctcgc gagcggagga
    12481 ttcgacgagt cgttccgcgc caacgaggac ggccacctct ggctccgcct ctcccgcgtg
    12541 acgggcgtgc accagacgag ggagccgctc cttctctacc ggcggcacgc ggacaacgcg
    12601 agcggcgacc tcctcgcgaa cgcccgcgat tccgtgcgct cgctcgagcg gctcgaggag
    12661 acgcacccgg agttcgcccg cgagctccgg cggccgatgc accgcctgcg cgggaaggag
    12721 tacctgcggc tcgggcgcga gctcctcgtg cggggcgagg atctccccgg cgcgcgcgcg
    12781 gcgctgcgca aggccgtcac gttccgtccg ggccggctgc gcggctggct ctacctcgcg
    12841 ttcgcctcgg tccccggcgg gcccggcgcc gtcgcggcct ttcggcggcg gga
//
LOCUS       DAOMFY010000092        12856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1501_length_12856_cov_6.659480, whole genome shotgun sequence.
ACCESSION   DAOMFY010000092 DAOMFY010000000
VERSION     DAOMFY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12856
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1501_length_12856_cov_6.659480"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..2217
                     /locus_tag="PLB01_09320"
     CDS             64..2217
                     /locus_tag="PLB01_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HQQ77541.1"
                     /translation="MFASAARGSWRRAAFAAILITAPPVLGQTPAKTPAPPPRLRIEI
                     GACEHALWANMPGGMSSNVELANAGTTPECAALALGSTRQALTASSVLSAAARSTSFT
                     VELLSKLNPWGLPADVAAKVLQAALESGGTLEGFSGQLGEKAMEQLANTLGGAAGGHG
                     PGGTAAREKVVGEFAEQAFKYFRGSEFVQTHTDRFHGMTCDGEFTVIVTASFLEARGE
                     VRIVANGDCHCAPFAGGVRVGAFAVVGVAPLTFGNLRKQGDEWVLTCTLGGPHYDVWA
                     PCCQVRDDHWVSPRETPPPVTAPPAQPTPTAAERKRAADRENVLRRCDPDGTRATEIF
                     RRGMHYDWKRADGAAESVLKAAREEVVRAQRPFCDCLNSMRADPALAGAGLTEVLDEL
                     RRLYCDPPAEVTPRPTPPPRVALPPCSAERDGYESARARWSNDPNNAGATLSMVRMRE
                     GLCACLRQQHGGRLPPDLEAFCNPRSHGNGASAPVLPAGPPAAGAPPQTAAGPPRFTA
                     PPTCSAGEPLKVAGTFDGDAGNTRVTVGGQTARVVTETSRDCVTQAPAGLSTGPVEIL
                     VTEGGRTTRLRSAAIRLRMSADQRDLLRGQSTKFRVSVEGLNGIPESAWSGGPDPQFL
                     DLAALERAAPGTRIPGRNEPGHILLTIENASRGTITLAKSEGEVIRLVIRRSDVPANG
                     TYVYDGVIQSLQDGRFNIKGAVFALVAPAAGEPPP"
     gene            2278..3195
                     /locus_tag="PLB01_09325"
     CDS             2278..3195
                     /locus_tag="PLB01_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HQQ77542.1"
                     /translation="MALKPTGSATISFGLVSIPVNLHSASESKAAISFNMLHETDHAR
                     VKQQLVCTKDGEIVERDKIVKGYEFEKGQYVVFTPAELKALDEQSNQVIEITEFVPLA
                     KIDPVYFDKGYYLSPGKGGDKAYRLLSRAMTQTGRCGLAKYAARGKQYLVVVRPVTGH
                     EGGLMMQQLNYADEIRSFTDVEVPAGDVNEAELKLAIMLTDQIATDEFHPENYKDEVK
                     ERVEGLIKRKIAGQEISEAEAPAPKTHVIDLMEALKASLAARTGRKGAARAADVPEKP
                     ERKPATRADATRGETRTKLRAVGNGKKRG"
     gene            3334..4155
                     /locus_tag="PLB01_09330"
     CDS             3334..4155
                     /locus_tag="PLB01_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77543.1"
                     /translation="MKGYSARDAASMLGVTLARVLTWAGWLGLDPSADLRLSFQDLVL
                     LRTAKGLAEAKVPAATVRRSLKRLRAQLPEGRPITGVRIAADGKRVVARDGAAVWNPE
                     SGQALFDFAVAEVATAAAPHARKAAEEARKEGEKLTAADWYRLGCDVEASSPADARDA
                     YRRAVELEPHHADAHVNLGRLLHEDGKTGAAEVHYRIALTADPKHAAAAFNLGVALED
                     RGKLREAAQAYERALASDADFLDAHYNLGLLYEKLGKPAAAIRHLSAYRQALDGL"
     gene            complement(4161..4418)
                     /locus_tag="PLB01_09335"
     CDS             complement(4161..4418)
                     /locus_tag="PLB01_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77544.1"
                     /translation="MTAPVRAMAAFGFAFFLSCLGFAIPKLQWFFYLPGLFMRRLMIL
                     IGLKTAIPGWTALGLEFGTNVAALFVVVFVTLSVFGLRRAG"
     gene            complement(4415..5377)
                     /locus_tag="PLB01_09340"
     CDS             complement(4415..5377)
                     /locus_tag="PLB01_09340"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQQ77545.1"
                     /translation="MAPPAPPPGGWPPVAVFGAGAVGCWFGGMLARAGAPVTLIGRPT
                     HVDAIAASGLRLESALFEARVHVAASTDPAAARGARLVLVAVKGYATEEAGRALAPHL
                     SGDATVLSLQNGVENAARLRAATGREAYPAVVWVAASMSAPGVVRHAGRGDLVIGDPG
                     WALPAAEARRLTLAEVAAVFEAAGVRCAVSDEIEAELWAKLAANCAFNPVSALARAPY
                     GRIGSTPGGRHLLRDVVAEVLAVAAASGVAIGARDVLGAVLKLADSMSEATSSMAQDL
                     ATGRRTEIDDLNGFVERRASELGVPTPINRTLAALVKLLEEPRT"
     gene            5461..5535
                     /locus_tag="PLB01_09345"
     tRNA            5461..5535
                     /locus_tag="PLB01_09345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5493..5495,aa:Gly,seq:ccc)
     gene            complement(5564..9157)
                     /locus_tag="PLB01_09350"
     CDS             complement(5564..9157)
                     /locus_tag="PLB01_09350"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQQ77546.1"
                     /translation="MDRVVGRLPFVRCTGAALSLFSALLIPARVEAQCLLTAAPSQFE
                     PNPPGPAAPRNVVFLSPVSFYHLSLYQNGAGGAWRMFMQESFGYSVLDLTNPAVPTVL
                     KYDYLPYASHPVSRNGDGQSFTQTVAASPDGQRVAIGLTSGGPANPPWNLVVGSPDGN
                     NGFTMWGDTAPVGAAGSVIVKSGSRYVHYGLAFDQLYVADVTSLPTFWAPNNVGFERP
                     GFPGGARLAAAGSYLLYTTSAGDLQVIDASNPGPVGSIATSLPRTTIPAASALFTGHS
                     LGAVKAAVDPANPNKLWVLVELQAGAGENSPSYTLFSLTESGGSFGAPVSSGAPFRVP
                     AAAGDLWSSAGGSSALAEHNGTIFVLMWAFRRSPTFQYVLYSTSTDAWGAQSPVTVTD
                     PTFSLGEMKTLSAGSTLWGYMASQNTAWILPMACSSPYAKATSVLAATNAATGATINS
                     GDAVVYGETITFVPQVLPVPAPGQRSDLTGFRWNFDFDFHTGALVEDAGDGVSPRLKN
                     PDDDQFLSPPYPPASATLVGPCDPRNLGVPSTGAGCWASVTSNTAQGGPDFTGSEAPG
                     ALKPLVVGFEANNTNGSNGPRLFTVNWKRPTIGLAATQILGGDPLVATYDGHPQSDGT
                     HPWKWYFDGSFAAYCTTGSACTPVGSAVTPGTHTYWVTVPYAQIGYSTPDYSGTAMGT
                     YTATSFVPVFAVNGTSTGPVTVPSNGTITVVNSSKRASGTTGTYQYCLVPAGNSCSAG
                     SYQAWSMTDPSSPSGSPASSATIPNPGGGNWLLKIRVSYTGGGTVDWPSANPATGIAL
                     TVTIRLNVTATATPNPANSGDYVTFACSATGGSGSYAYSWDGQFGQVATTQNYIIRMT
                     NGSATPQNTTLTCTATDTLASAFGTADATATVLPVPPPSVTATATPNPAGNGSSVTFN
                     CSATGGTGTGYTYAWTGPSGATVATTPSFSQAFSNSTVSNQTFAHTCSVTDSGGKTGT
                     STVGLVVLPYPLAVTVTAPSPASAGNPAQLTCLAAGAGPFTYTWSLSDGGSGTGSPLV
                     HTFLNPGAYTATCTALDPATGYSGQKTVTVNATLPPGAGGPYLLAVLSPCRVLDTRTA
                     AGPLGGPAILPAGYPDRAFLVAGACGIPNDAKAISVNVTVTNVTAPGTMLVYRGDGAP
                     TGASTASLLPGKTRANNAFVQLAMDGSGTIKVLNTSAGTFDLIVDVNGYFR"
     gene            complement(9344..9928)
                     /locus_tag="PLB01_09355"
     CDS             complement(9344..9928)
                     /locus_tag="PLB01_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016870390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HQQ77547.1"
                     /translation="MSKKILMIVGDFVEDYEVMVPFQALQMVGHTVHAVCPGKKAGDK
                     VRTAIHDFEGDQTYSEKPGHNFALNCAFDDVKESAYDALVIPGGRAPEYLRLNPRVLE
                     IVRHFMAGDKPVAAICHGAQILAAAGVLDGKKSSAYPAVGPEVTRAGGRYAEIAVDQA
                     VTDGNLVSAPAWPAHPVWLSQFTKLLESRVAAHA"
     gene            9986..10201
                     /locus_tag="PLB01_09360"
     CDS             9986..10201
                     /locus_tag="PLB01_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HQQ77548.1"
                     /translation="MREILDKIPWQLAILAALTLGLAPFKPPHVVEKLEMLFKGTLVK
                     PIDWLDLVMHGTPWLIVILKGAFELKR"
     gene            complement(10217..11155)
                     /gene="hflC"
                     /locus_tag="PLB01_09365"
     CDS             complement(10217..11155)
                     /gene="hflC"
                     /locus_tag="PLB01_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQQ77549.1"
                     /translation="MKRIILAAGLFLGAVLLVSSVYTLSETEQAVLTQFGRPVGGVVK
                     DPGLHLKVPFIQEVHRFDRRWLEYDGDPNEIPTKDKKYIWVDTYARWRISDPLRFFQA
                     VRDERGGQSRLDDIVDGETRNAIASFDLIEVVRSTNRPFQVTEDLEGIGAAEAMAKVS
                     AGRSGIAKIILEKASKITPEFGIELVDVRFKRINYVDSVQQKVFERMISERKRIAERS
                     RSEGQGRAAEIRGQKERDVLGASSSGYKTAQEVKGIADAKATSVYAKAYGRDADLYQF
                     LKSMETLGGSVDEKTWLILSTNSELLKYLKSDGGPT"
     gene            complement(11152..12177)
                     /gene="hflK"
                     /locus_tag="PLB01_09370"
     CDS             complement(11152..12177)
                     /gene="hflK"
                     /locus_tag="PLB01_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQQ77550.1"
                     /translation="MSEQGWSVDLGGGKRLEFPPVPPIPKIPPGAARIVLIAAAALLV
                     VVTGYYQVEPDEVAVVQRFGKFVRTSEPGPHLKVPFGVERITKVPVQRQLKMEFGFRT
                     AHAAAQTSYQAPTKETIAESQMLTGDLNVAVVEWIVQFRIKDPAAFLFRVRDVPETFR
                     YMSEAAMRQVVGDHSVDEVLTIGREAIALQAKVELQRLCELYGIGIEVQQLVLQDVNP
                     PGPVKPAFNEVNQAIQEKERAINDAWADYNKAVPKAKGEAEQAVRAAEGYALERVNNA
                     EGDAKRFEALYDEYKKAPAVTRRRIYLETLNATLPKAGRKLILDDSARGILPLLNLEG
                     EKKEVKP"
     gene            complement(12199..>12856)
                     /locus_tag="PLB01_09375"
     CDS             complement(12199..>12856)
                     /locus_tag="PLB01_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQQ77551.1"
                     /translation="TVTDLPRGAALGVARAAGCSLTRASLAFAEIAFRPQGVPRSINP
                     RLMPGDEACRKTALVLFGLSRSVPDSPPPAGTPDRLAVVLDPDALAAMDEASADAPVP
                     RLVEGGVKEPKEVRRVKPAYPESLRRQRLQGAAVLTARIDREGRVADVRVVEPAGTLD
                     TLTRDGTSLDLEALRAVALWRYEPARLDGEPVPVLLSVRITWAVSGTGMGSPDASMRR
                     "
BASE COUNT         1927 a         4586 c         4441 g         1902 t
ORIGIN      
        1 cccgcgcccc cttcgcgtag actccgtctc aacacaagag tcggcctcac gaggaggtgg
       61 tgcttgttcg cttccgcagc tcggggctcg tggcggcgcg ccgcgttcgc cgcgatcctg
      121 atcacggccc ctccggtcct cgggcagacc cccgcgaaga ctccggctcc gccgccccgc
      181 ctgcgcatcg agatcggcgc ctgcgagcat gcgttgtggg ccaacatgcc gggcggcatg
      241 agctccaacg tggagctcgc caacgccggg acgacgcccg aatgcgcggc gctcgcgctc
      301 gggtcgacgc gccaggctct cacggcctcg tccgtactca gcgccgcggc ccggagcacg
      361 tcgttcaccg tcgagctcct ctccaagctc aacccgtggg gtcttccggc cgacgtcgcg
      421 gcgaaggtcc tccaggccgc gctcgaatcc ggagggacgc tcgaaggctt cagcggccag
      481 ctcggcgaga aagccatgga acagctcgcg aacaccctcg gcggcgcggc cggcgggcac
      541 gggccgggcg gcaccgcggc ccgcgagaag gtcgtcggag agttcgcgga gcaggccttc
      601 aagtacttcc gcggttcgga gttcgtccag acgcacacgg acaggttcca cgggatgacc
      661 tgcgacggcg agttcaccgt catcgtgacg gcctcgttcc tcgaggcgcg cggcgaggtc
      721 cgcatcgtgg cgaacggcga ctgccactgt gcgccgttcg cgggcggcgt ccgcgtcggc
      781 gcgttcgccg tcgtcggcgt cgcgccgctc acgttcggca acctccggaa gcagggagac
      841 gagtgggtcc tcacgtgcac gctcggcggc cctcactacg acgtctgggc cccgtgctgc
      901 caggtccgcg acgaccactg ggtctcgccg cgcgagacgc cgccgcccgt gacggcgccg
      961 ccggcccagc caacgcccac cgcggcggag cgcaagcgcg cggccgaccg cgagaacgtc
     1021 cttcggcggt gcgacccgga cgggacgcgc gcgaccgaga tcttccggcg tggaatgcac
     1081 tacgactgga agcgggccga cggggccgcc gagagcgtcc tcaaggcggc gcgcgaggag
     1141 gtcgtgcgcg cccagcggcc cttctgcgac tgtctcaact cgatgcgcgc cgacccggcg
     1201 ctggccgggg cggggctcac ggaggtcctc gacgagctgc gccgcctgta ctgcgatccg
     1261 ccggccgagg tcacgccgcg gccgacaccg ccgccgcgcg tcgcgctccc gccttgctcg
     1321 gccgagcgcg acggctacga gagcgcacgc gcgcgctggt cgaacgaccc caacaacgcg
     1381 ggggccactc tgtcgatggt tcgcatgcgc gagggcctct gcgcctgcct caggcagcag
     1441 cacggcggga ggctcccgcc cgacctcgag gcgttctgca acccgcggag ccacggcaac
     1501 ggtgcgtccg cgccggtcct cccggcgggc cctccggccg ccggcgctcc tccgcagacg
     1561 gccgcggggc cgccgcgatt caccgctccg ccgacgtgca gcgcgggcga gcccctgaag
     1621 gtcgccggga cgttcgacgg cgacgcgggc aacacccgtg tgaccgtcgg cggccagacg
     1681 gcgcgcgtcg tgaccgagac ctcccgcgac tgcgtcacgc aggctcccgc agggctttcg
     1741 accgggccgg tcgagatcct cgtgacggag ggcggtcgaa ccacgcgact gaggtccgcg
     1801 gcgatccggc tccgaatgag cgccgaccag cgggacctcc tgcgcggcca gtccacgaaa
     1861 ttccgcgtct ccgtcgaggg cctgaacggc atcccggagt ccgcctggag cggaggtccg
     1921 gatccgcagt tcctggacct ggccgcactc gaacgggccg cgcccggaac ccggatcccc
     1981 ggccggaacg agccgggcca catccttctg acgatcgaga acgcctcgcg cggcacgatc
     2041 actctggcaa agagcgaagg cgaggtgatc cgcctcgtga tccggcgaag cgacgtcccg
     2101 gcgaacggga cgtacgtcta tgacggcgtc atccagtcgc tccaggacgg gcgcttcaac
     2161 atcaagggcg cggtgttcgc gctcgtcgcc cccgcggccg gagaaccgcc gccctgaggc
     2221 ctcccgaggc cggcactgag aaccattcgc gcgcggaggc gtagaatcag gggtcttatg
     2281 gcactcaagc cgaccggttc cgcgaccatc tctttcgggc tcgtctcgat tccggtgaac
     2341 ctgcactcgg cgagcgagtc gaaggctgcc atctccttca acatgctcca cgagaccgac
     2401 cacgcgcgcg tcaagcagca gctcgtctgc acgaaggacg gcgagatcgt ggaacgggac
     2461 aagatcgtga agggctacga gttcgagaag ggccagtacg tcgtcttcac gcccgcggag
     2521 ctcaaggccc tcgacgaaca gtcgaaccag gtcatcgaga tcaccgagtt cgtgccgctc
     2581 gcgaagatcg acccggtcta tttcgacaag ggctactacc tctcgcccgg caagggcggc
     2641 gacaaggcct accgcctcct ctcgcgcgcg atgacccaga cgggccgctg cgggctcgcg
     2701 aagtacgcgg cgcgcggcaa gcagtacctc gtcgtcgtgc ggcccgtcac gggccacgag
     2761 ggcgggctca tgatgcagca gctgaactac gccgacgaga tccgttcctt cacggacgtc
     2821 gaggtgcccg ccggcgacgt caacgaggcc gagctgaagc tcgccatcat gctcacggac
     2881 cagatcgcga ccgacgagtt ccaccccgag aactacaagg acgaggtcaa ggagcgcgtc
     2941 gaggggctca tcaagaggaa gatcgccggt caggagatct ccgaggccga ggcgcccgcg
     3001 ccgaagacgc acgtcatcga cctcatggag gccctgaagg cgagcctcgc ggcgcgcacg
     3061 ggccgcaagg gcgccgccag ggccgcggac gttccggaga agcccgagcg caagcccgcg
     3121 acccgcgccg acgcgacgcg cggcgagacc cggacgaagc tgcgcgcggt cgggaacgga
     3181 aagaaaagag gctagagacc ccggggcacg aggattttct tagaaggtat gagtccttcg
     3241 tcttcgtccc cccgtcttac cttctaagca gatctctctc ttttctcttc ctcatttctc
     3301 ttttcttccg agcaaatctt ctattctttc ttcgtgaaag gctattcggc ccgcgatgcg
     3361 gccagcatgc tcggcgtcac gctcgcgcgc gttctcacgt gggcgggctg gctcgggctc
     3421 gatccttccg cggacctccg cctctcgttc caggacctcg tcctcctgcg caccgccaag
     3481 ggcctcgccg aggcgaaggt ccccgccgcg accgtgcgcc gctcgctcaa gcgactgcgc
     3541 gcgcagcttc cggagggccg cccgatcacg ggagtgcgca tcgcggccga cggcaagcgc
     3601 gtcgtcgccc gcgacggcgc cgccgtgtgg aacccggaat ccggtcaggc tctcttcgac
     3661 ttcgcggtcg ccgaggtcgc gaccgcggcc gccccccacg cccgcaaggc ggccgaggag
     3721 gcgcgcaagg aaggagaaaa gctcaccgcc gccgactggt accgcctcgg ttgcgacgtc
     3781 gaggcgtcct cccccgccga cgcgcgcgac gcgtaccgcc gcgccgtcga gctcgagccc
     3841 caccacgcgg acgcgcacgt gaacctcggg cgcctcctgc acgaggacgg gaagacgggc
     3901 gcggcggagg tccactaccg catcgcgctc accgcggacc cgaagcacgc cgccgccgcg
     3961 ttcaacctcg gcgtcgcgct cgaggaccgc gggaagctgc gcgaggccgc gcaggcgtac
     4021 gagcgtgcgc tcgcctcgga cgccgacttc ctcgacgccc actacaacct cggactcctc
     4081 tacgagaagc tcgggaagcc cgcggccgcg atccggcacc tctcggcgta caggcaggcg
     4141 ctggacgggc tctgagccgc tcagcccgca cgccgcaagc cgaagaccga cagcgtgacg
     4201 aagaccacga cgaagagagc ggccacgttc gtcccgaact cgaggccgag ggccgtccag
     4261 cccgggatcg cggtcttcaa gccgatcagg atcatcagcc ggcgcatgaa gaggccgggc
     4321 aggtagaaga accactgcag cttcggaatc gcgaaaccga ggcagctcag aaagaatgcg
     4381 aatccgaacg cggccatcgc gcgcaccggc gccgtcacgt gcggggctcc tcgaggagct
     4441 tcacgagggc ggcgagcgtc cggttgatcg gcgtcgggac gccgagctcg ctcgcccgcc
     4501 gttcgacgaa cccgttcagg tcgtcgatct ccgtccggcg gcccgtggcg aggtcctgcg
     4561 ccatcgagga cgtcgcctcc gacatcgagt ccgcgagctt cagcacggcg ccgaggacgt
     4621 cccgcgcccc gatcgcgacg ccgctcgcgg ccgcgacggc gaggacctcg gcgacgacgt
     4681 ccctcaggag gtggcgcccc cccggcgtcg agccgatccg gccgtacggc gcgcgcgcga
     4741 gagccgaaac ggggttgaac gcgcagttcg cggcgagctt cgcccagagc tcggcctcga
     4801 tctcgtccga cacggcgcac cggacgccgg cggcctcgaa gaccgcggcg acctcggcga
     4861 gcgtgagcct ccgggcctcg gcggccggaa gcgcccagcc cggatccccg atcacgaggt
     4921 cgccgcgtcc cgcgtgccgg acgacgcccg gggcggacat cgaggccgcc acccagacga
     4981 ccgccgggta cgcctcgcgg cccgttgcgg cgcgcagcct cgcggcgttc tcgacgccgt
     5041 tctgaaggct caggacggtc gcgtccccgg agaggtgcgg cgcgagggcc cgtccggcct
     5101 cctccgtcgc ataccccttc acggccacga ggacgagccg ggccccgcgg gccgcggcgg
     5161 gatcggtcga cgccgcgacg tgcacgcggg cctcgaagag cgcggactcg aggcggagtc
     5221 ccgacgccgc gatcgcgtcg acgtgagtcg ggcggccgat gagcgtgacc ggcgcgcccg
     5281 cgcgcgccag catcccgccg aaccagcagc cgacggcgcc cgcgccgaag acggcgacgg
     5341 gcggccagcc gccgggaggg ggcgctgggg gtgccacggg tgaagtgtgc caccgggagg
     5401 ccgccgcctc tccttgacgc cgcctcccgt cgcgcgctac aacctccgtc cggccctcga
     5461 gcgggtgtag ttcagtggta gaacgtcagc ttcccaagct gaatgtcgtg ggttcgatcc
     5521 ccatcacccg ctccacttcg tgccctccgc ccccgatcgc ccgtcagcgg aagtagccgt
     5581 tcacgtccac gatcaggtcg aaggtgccgg ccgacgtgtt cagaaccttg atcgtgcccg
     5641 agccgtccat cgcgagctgc acgaacgcgt tgttggcgcg cgtcttcccc ggcaggaggc
     5701 tggccgtgct cgcgcccgtg ggcgcgccgt cgccgcggta gacgagcatg gtccccggcg
     5761 ccgtcacgtt cgtcaccgtg acgttgaccg agatcgcctt cgcgtcgttc gggattccgc
     5821 acgcccccgc gacgaggaac gcgcggtcgg gatagcccgc cggcaggatc gcgggcccgc
     5881 cgaggggccc ggcggcggtg cgcgtgtcga ggacccggca gggactgagc accgcgagca
     5941 gatagggccc gcccgccccc ggcggaagcg tcgcgttcac ggtcaccgtc ttctggcccg
     6001 agtacccggt cgcgggatcg agcgccgtgc aggtcgccgt gtaggcgccc gggttcagga
     6061 acgtgtgaac cagcggcgaa ccggtcccgg agcctccgtc ggacagcgac cacgtgtacg
     6121 tgaacggtcc cgcccccgcg gccaggcacg tcagctgagc cgggttgccg gcggacgcgg
     6181 ggctcggcgc cgtgacggtc accgccagcg ggtacggaag gaccacgaga ccgaccgtcg
     6241 aggtccccgt ctttcccccg ctgtccgtga cggagcacgt gtgtgcgaac gtctggttcg
     6301 agaccgtgga gttgctgaag gcctggctga aggacggcgt cgtggcgacg gtcgcacccg
     6361 aaggtcccgt ccacgcgtac gtgtagcccg tgccggttcc acccgtcgcc gagcagttga
     6421 acgtcacgct cgagccgttg ccggccgggt tcggcgtcgc cgtcgccgtc accgacggcg
     6481 gcggaacggg aaggaccgtg gccgtggcgt cggcggtgcc gaacgcgctc gcgagcgtgt
     6541 ccgtcgcggt gcacgtcagc gtggtgttct ggggagtggc gcttccgttc gtcatccgga
     6601 tgatgtagtt ctgcgtcgtg gcgacctgtc cgaactggcc gtcccagctg taggcgtagc
     6661 tcccggaccc gcccgtggcc gagcacgcga aggtgacgta gtcaccgctg ttggcagggt
     6721 tcggggtcgc cgtcgccgtt acgttgagcc ggatcgtcac cgtgagcgcg atccccgtcg
     6781 cgggattcgc gctcggccag tccacggtgc ctcctcccgt gtagctgacc cggatcttca
     6841 ggagccagtt gcccccgccc ggattcggga tcgtggccga gctcgccggg ctgcccgacg
     6901 gcgaagacgg atcggtcatc gaccacgcct ggtagctgcc ggccgagcac gagttccccg
     6961 cggggacgag gcagtactgg taggtccccg tcgtcccgct cgcccgcttc gaggagttga
     7021 cgaccgtgat cgtcccgttc gaggggaccg tcaccgggcc cgtggacgtt ccgttcacgg
     7081 cgaagaccgg gacgaaggac gtggccgtgt aggtgcccat cgccgtcccg gagtagtccg
     7141 gggtcgagta cccgatctgc gcgtacggaa cggtgaccca gtacgtgtgc gtccccggcg
     7201 tcacggcgga tccgacgggc gtgcacgcgg accccgtcgt gcagtacgcc gcgaacgacc
     7261 cgtcgaagta ccacttccac ggatgcgtcc cgtcggattg cgggtggccg tcgtaggtcg
     7321 cgacgagcgg atcgccgccg agaatctgcg tcgcggcgag tccgatcgtg ggccgcttcc
     7381 agttcaccgt gaagagccgc ggcccgttgc tgccgttcgt gttgttcgcc tcgaagccca
     7441 cgacgagcgg cttgagcgcc cccggcgctt ccgaccccgt gaagtccggg ccgccctgcg
     7501 ccgtgttcga cgtgacggac gcccagcagc ccgcgccggt cgacgggacg ccgaggttgc
     7561 gcggatcgca gggcccgacg agcgtcgcgg acgcgggcgg atacggaggg gacaggaact
     7621 ggtcgtcgtc ggggttcttc aggcgcgggg acacgccgtc gccggcgtcc tccacgagcg
     7681 ctcccgtgtg gaagtcgaag tcgaagttcc agcggaagcc cgtgaggtcg gagcgctgac
     7741 ccggagcggg caccggcagc acctgcggca cgaacgtgat cgtctcgccg tacacgaccg
     7801 catcgccgga attgatcgtc gctccggtcg cggcgttcgt cgccgcgagg acggacgtcg
     7861 ccttcgcgta cggggacgag caggccatcg ggaggatcca ggccgtgttc tgcgaggcca
     7921 tgtaccccca gagggtgctt cccgccgaca gggtcttcat ctcgccgagg gagaacgtgg
     7981 gatccgtgac cgtgaccgga ctctgggcgc cccacgcgtc cgtcgacgtc gagtagagga
     8041 cgtactggaa ggtcggcgag cgccggaagg cccacatgag gacgaagatc gtcccgttgt
     8101 gctcggcgag cgcggacgag cccccggcgg acgaccacag gtctcccgcc gccgcgggca
     8161 cgcggaacgg cgcgccggac gagacggggg cgccgaagct tccgccgctc tccgtcaacg
     8221 agaagagcgt gtagctcggc gagttctcac ccgccccggc ctgcagctcg acgaggaccc
     8281 acagcttgtt cggattcgcc gggtcgacgg cggccttcac ggcgccgagc gagtggccgg
     8341 tgaacagcgc cgatgccgcg ggaatcgtcg tgcgcggaag gctcgtcgcg atgctcccca
     8401 cgggacccgg gttcgaggcg tcgatgacct ggaggtcgcc ggcgctcgtc gtgtagagga
     8461 ggtagcttcc ggcggcggcg agcctcgccc cacccgggaa ccccggccgc tcgaagccga
     8521 cgttgttcgg cgcccagaac gtcggaaggc tcgtgacgtc cgccacgtag agctggtcga
     8581 atgccagccc gtaatggacg tagcggctcc cgctcttcac gatcaccgac ccggcggcgc
     8641 ccaccggcgc cgtgtcgccc cacatcgtga agccgttgtt cccgtccgga gagccgacca
     8701 ccaggttcca cggagggttc gcggggccgc cggacgtcag gccgatcgcg acgcgctgtc
     8761 cgtcgggcga cgcggcgacg gtctgcgtga agctctggcc gtccccgttt ctcgagaccg
     8821 ggtgcgacgc gtaagggagg tagtcgtact tcaggacggt cggcaccgcc gggttcgtca
     8881 ggtcgagtac cgagtagccg aacgactcct gcatgaacat gcgccacgcc ccgcccgcgc
     8941 cgttctggta gagcgacagg tggtagaagg aaaccgggct gaggaaaacc acgttccgag
     9001 gcgcggcagg gcccggcggg ttcggctcga actgcgaggg cgccgcggtc aggagacact
     9061 gggcctcgac gcgcgccggg atgagcaggg ccgagaaaag ggaaagtgcg gcccccgtac
     9121 accgtacgaa cgggaggcgg ccgacgaccc tgtccatagc gtcctgcggc ccttccgact
     9181 gcagagcttg tgccaacttg gtgaacaccg tccggagcgg cccgacggtg ccctggctca
     9241 cgccgcggtg ggaatggcga agcccgaccg acgcgaattg tcggagtcgt ccacgcgccg
     9301 ccgccattcc gcggcgactt ccagcggggt caggctcttc tcgtcaggcg tgcgcggcga
     9361 cccgcgactc gaggagcttc gtgaactggg acagccagac gggatgggcc ggccacgcgg
     9421 gcgccgagac caggttcccg tccgtcacgg cctgatccac ggcgatctcg gcgtaacggc
     9481 cgccggcgcg cgtcacctcc ggaccgaccg ccggataggc cgaggacttc ttcccgtcga
     9541 gcacgcctgc ggccgcgagg atctgcgcgc cgtggcagat cgcggcgacc ggcttgtcgc
     9601 cggccatgaa gtgccggacg atctcgagga cgcgcgggtt gagacgcagg tactccggcg
     9661 cgcgccctcc ggggatgacg agcgcgtcgt aggcggattc cttcacgtcg tcgaaggcgc
     9721 agttgagagc gaagttgtgg cccggcttct cgctgtaggt ctggtcccct tcgaaatcgt
     9781 ggatcgccgt ccgcaccttg tcgcccgcct tcttgcccgg acagacggcg tgcaccgtgt
     9841 gcccgaccat ctggagagcc tggaacggca ccatcacctc gtagtcctcg acgaagtcgc
     9901 ccacgatcat caggatcttc ttcgacatgg ccttcctcct tctcgggtcc gcctcagtat
     9961 cttcccgcgt tagtctccgc actgcatgcg cgaaatcctc gacaagatcc cctggcagct
    10021 cgcgatcctg gcggccctca cgctcggcct cgcgccgttc aagccgccgc acgtcgtcga
    10081 gaagctcgag atgctgttca agggcacgct cgtgaagccg atcgactggc tggacctcgt
    10141 catgcacggc acgccgtggc tgatcgtgat cctcaaggga gcgttcgagc tgaagcgctg
    10201 aggggccggc tccgtcctac gtcggccccc cgtcgctctt gaggtacttc agcagctcgg
    10261 agttcgtgga caggatgagc cacgtcttct cgtccacgct gcccccgagg gtctccatcg
    10321 acttcaggaa ctggtagagg tccgcgtcgc ggccgtaggc cttcgcgtac accgacgtgg
    10381 ccttcgcgtc cgcgattccc ttgacctcct gggcggtctt gtatccgctg gacgacgccc
    10441 cgaggacgtc gcgctccttc tggccgcgga tctcggcggc gcgaccctgg ccctccgagc
    10501 ggctgcgctc cgcgatgcgc ttgcgctccg agatcatccg ctcgaagacc ttctgctgga
    10561 cgctgtccac gtagttgatg cgcttgaacc gcacgtccac gagctcgatc ccgaactcgg
    10621 gcgtgatctt cgaggccttc tcgaggatga tcttcgcgat tccgctccgt ccggcgctca
    10681 ccttcgccat ggcttcggcc gccccgatcc cctcgaggtc ctccgtgacc tggaacggcc
    10741 ggttcgtcga gcggacgacc tcgatcaggt cgaaggaggc gatcgcgttg cgcgtctcgc
    10801 cgtccacgat gtcgtcgagg cggctctgtc cgccgcgctc gtcccggacc gcctggaaga
    10861 accgcagcgg gtcggagatc cgccagcggg cgtacgtgtc gacccagatg tacttcttgt
    10921 ccttcgtggg gatctcgttc gggtctccgt cgtactcgag ccatcgccgg tcgaagcggt
    10981 ggacctcctg gatgaacggg accttcaggt gcagtcccgg atccttcacg acgcctccga
    11041 cgggccggcc gaactgcgtg agcacggcct gctcggtctc ggagagcgtg tagacggaag
    11101 aaacgagaag cacggcgccg aggaagaggc ccgccgcgag gatgatccgc ttcacggctt
    11161 cacctccttc ttctctcctt cgagattgag gagcgggagg atccccctcg cgctatcgtc
    11221 caggatcagc ttgcgtcccg ccttcgggag cgtggcattc agggtctcga ggtagatgcg
    11281 ccggcgcgtg acggcgggcg ccttcttgta ctcgtcgtac agggcctcga aacgcttggc
    11341 gtcgccctcg gcgttgttca cgcgctcgag cgcgtacccc tctgcggccc ggacggcctg
    11401 ctcggcctcg cccttggcct tgggaacggc cttgttgtag tcggcccacg cgtcgttgat
    11461 cgcgcgctcc ttctcctgga tcgcctggtt gacctcgttg aacgccggct tcacggggcc
    11521 gggaggattc acgtcctgca ggacgagctg ctggacctcg atgccgatcc cgtagagctc
    11581 gcagagccgc tggagctcca ccttggcctg cagcgcgatc gcctcgcgcc cgatcgtgag
    11641 gacctcgtcg acgctgtggt cgccgacgac ctgccgcatc gccgcttccg acatgtaccg
    11701 gaacgtctcg gggacgtcgc gcacgcggaa gaggaacgcc gccgggtcct tgatgcggaa
    11761 ctggacgatc cactcgacga cggcgacgtt gaggtccccc gtgagcatct gcgactcggc
    11821 gatggtctcc ttcgtcggcg cctggtagga cgtctgagcg gccgcgtgcg cggtccggaa
    11881 tccgaattcc atcttgagct ggcgctggac cggcaccttc gtgatgcgct ccacgccgaa
    11941 cggcaccttg aggtgagggc ccggctcgct cgtccgcacg aacttgccga acctctggac
    12001 gacggcgacc tcgtccggct ccacctggta gtaaccggtc accacgacga gaagggccgc
    12061 ggcggcgatc aggacgatgc gcgccgcacc gggcggaatc ttcgggatcg gcgggaccgg
    12121 cggaaactcc agccgcttgc cgccacccag gtcgacgctc cagccttgtt cggacatgtc
    12181 ccgttccttt cggccgcgtc agcggcgcat cgatgcatcc ggcgagccca tgcccgtccc
    12241 ggacaccgcc cacgtgattc tcaccgagag caggacaggc acgggttctc cgtccaggcg
    12301 ggccggctcg taacgccaga gcgcgaccgc cctgagtgcc tcgaggtcga ggctcgtccc
    12361 gtcccgggtc agagtatcga gcgttccggc cggctcgacg acgcgcacgt cggccaccct
    12421 cccctcccgg tcgatccggg ccgtgagcac cgcggcgccc tgcaaacgct gacggcggag
    12481 cgattccggg tacgccggct tcacgcggcg aacctctttc ggctccttca ctccgccctc
    12541 gaccagccgc ggcacgggag cgtcggcgga agcctcgtcc atcgcagcga gggcgtccgg
    12601 gtcgaggacg accgcgagcc ggtcgggcgt tccggcgggc ggaggcgaat cgggaaccga
    12661 ccgcgaaagc ccgaagagga cgagcgccgt cttccggcag gcttcgtcgc ccggcatgag
    12721 gcgcgggttg atcgatcggg gcacgccctg cggccggaac gcgatctcgg cgaaggcaag
    12781 ggatgcgcgc gtgagcgagc agcccgcggc tcgcgccacg ccgagggccg cgccgcgcgg
    12841 caggtccgtc accgtg
//
LOCUS       DAOMFY010000093        12770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1516_length_12770_cov_9.641526, whole genome shotgun sequence.
ACCESSION   DAOMFY010000093 DAOMFY010000000
VERSION     DAOMFY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_12770_cov_9.641526"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLB01_09380"
     CDS             complement(<1..516)
                     /locus_tag="PLB01_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77552.1"
                     /translation="MDGGRADLEGRVALLESALKDVSARLAALEGRTGPIQVRPAAVE
                     TAVPAAPAIAAAAVPGGPVPSQAEEGAPVANAITLAGRSFLVLGGAFLLRTLTEAGRV
                     PVVVGALLGLVYALAWVVLALRDGRRGANASADVHGLTAAIVAFPLVGEAATRMGAFT
                     PAAAAVALAVVT"
     gene            complement(533..1729)
                     /gene="nrfD"
                     /locus_tag="PLB01_09385"
     CDS             complement(533..1729)
                     /gene="nrfD"
                     /locus_tag="PLB01_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQQ77553.1"
                     /translation="MEESLLHAVQGYMYPNEVELQWSILIVLYPFTTGLVAGAFILAS
                     LERVFNVAAVKPTYRLALLSALAFLIVAPLPLQLHLGHPERSFEMYLTPHTSSAMAMF
                     GFVYLWYLMAVLVLEIWLDYRKDIVLMSRSETGWKKTLYKALSLGVDNISEESLRIDD
                     KVGRFITIIGIPSAFLLHGYVGFIFGSIKANPWWSTPVMPIVFLFSAIVSGIALVMLM
                     YMAVCVLKGVPIDMPCVDTIARYLFYAFLIDFTIELMDLIHRIYEADESFQSLDFMVH
                     TRLYTSQILIQIVFGTIVPLGLLAFTQLVKLSESARKGIYVFAGCLTLMGIFAMRWNV
                     VIGGQLFSKSFLGYTTYKMAFATKEGLLPAIVLMILPFVILWALVKILPPFEEPHEPK
                     AEPAAA"
     gene            complement(1734..2498)
                     /locus_tag="PLB01_09390"
     CDS             complement(1734..2498)
                     /locus_tag="PLB01_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ77554.1"
                     /translation="MSQDRREFLATAGKVLVLTAPAAAYWDALAAGKTEAQESAYRMQ
                     DHFWGMIVDIDKCIGCGGCVRACKTENNVPLEPFYFRTWVERYHVEPLSEHPEVDSPN
                     GGYNGFPEKFASGDKAKSFFVPKLCNQCAHSPCTQVCPVGATFEAPDGAVLVDKTYCL
                     GCRYCVQACPYGCRFIDPRTETVDKCNLCYHRITKGLTTACCENCPTGARQLVDLKDP
                     RDPVHEFLRTHKVHVLKPQMATGSKLFYNALDGSVR"
     gene            complement(2495..2965)
                     /locus_tag="PLB01_09395"
     CDS             complement(2495..2965)
                     /locus_tag="PLB01_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HQQ77555.1"
                     /translation="MNRLFKDKEHLVRVAGLFLAGIVVFVIARAILVPKGFGDYGHYR
                     AGALADVAARPVAYAGRAACVECHTDEGDRVKGGKHKNVGCESCHGALAKHAADPSAL
                     KPAKPDAKTLCQTCHLVNVAKPAKFPQIDPKSHMDGAPCLTCHDQHAPDKEPKK"
     gene            complement(2962..5937)
                     /locus_tag="PLB01_09400"
     CDS             complement(2962..5937)
                     /locus_tag="PLB01_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQQ77556.1"
                     /translation="MSATPARNLATRAWEAVDERFHLSGLVEFLSHKEVPVAHSIVWY
                     YLGGLSLFLFVVQIGTGILLLMNYQVGENTSYESMKHLVGRVPFGWLIRSIHCWSAHL
                     MSLAVLAHLFSVLFVKAYRKPRELTWYTGFAMLGLVLGFGFSGYLLPWNELSYFATAV
                     GTDSLKAIPLVGPWLLRVLRGGDEVSVQTLYRFFALHVCILPLVIAGLIGAHLLFIQR
                     QGMAEPMETGHEPLEKKRGMPFFPNFALRDLLLWVIVLNVLALLAVILPFGPGIPGME
                     WELGEKANPLKPAYPGIKPEWYFLWVYQMLKEFPAHIAGMEGPQACILLASILGGIAF
                     FIPLLDRNAAKGKPSPLFTDLGVAGLLFLGFLTVKAWDVGVTVPKGKDPTADPVLANT
                     IARTAALWILGIGIAVTVLRRLKWKHSDFDFTIAVLLQAALNGFAHLSWLVSGGIALA
                     ALAAMTIFRRVKGSGAVAASVLVLAALVAPQLRADEKGAAPAAAAAAPAGHVGETAWP
                     ADFKKMFEAAEKGVPVLDEKARARFKALPTHAQELFFAAAKAGTLSGATHLAALLALD
                     IDDRKVELILGDNCFLCHSNPDLPDEILFRKREKGDPLAHLEIREVVSDAHVRGGLMC
                     AGCHGGKPTDKEMTAEIGKRWPSSEVRHKDRSWIPEFCARCHSSSEFMRAYNPSLPVD
                     QLLKYRTSKHGQLLLEKKDSKAAQCVSCHGVHGIRPPDSTTSLVYRENIPSTCGKCHA
                     DAAYMKGYVLDDGKTPLPTNQLEQYKKSVHGIALLVKHDVGAPACNSCHGNHAALPPQ
                     VAFVSQVCRNCHAANGSLFDGSPHKKSFEKNGWPECETCHGKHDIQRPTDEMLSDDPK
                     GLCHACHAKYGQPKCDETAKYFYASITSLRTSHETLNHDVDRLLERGFDVDELRFQSS
                     AVNDALRKTRLGIHTFDRSDFTRNSDATKAALDALKAGTANVWREYRFRRNGLLLATV
                     LISVFGVLLYLKIREVDRR"
     gene            complement(5934..6407)
                     /locus_tag="PLB01_09405"
     CDS             complement(5934..6407)
                     /locus_tag="PLB01_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQQ77557.1"
                     /translation="MENSLSREPDAAFSRRRWIDVLLGSSFVAWGLAVVYPVLRYLTP
                     AKDAGGANKVTLTDAQKQELGTNGFLIARLGSERVLLLKDKDQKLRAMSAKCTHEGCT
                     VQFVPADGIVWCACHNGKFAMDGRVISGPPPRPLAAFTVEGDLKGAVTVSKGSAA"
     gene            6588..9047
                     /locus_tag="PLB01_09410"
     CDS             6588..9047
                     /locus_tag="PLB01_09410"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQQ77558.1"
                     /translation="MLFAPVLAAVAALSAPVAPASVGMSGGRLADLDAAIADSIAKKE
                     CPGAVVLVGRHGKVVFRKAYGNRAVVPAVEPMTVDTVFDLASLTKVVATATSVMTLVE
                     DGKIRLQDRVAKLIPEFAAGGGARDQVTVEELLTHRAGLVADDPMALYVGTPQEIFER
                     KYRQPLVNAPGAKFVYSDAGYEVLGEILRKVSGLRLDEYAAKRVFEPLGMKETGFLPL
                     SPSSPSRKSSPSLPLSRIAPTERINGEIRRGAVHDPRAYALGGVAGHAGLFSTADDLA
                     KFCAAMLRGGGGVLSPAGVASMLRPRFYGDGVLRGLGWDVGSSYSTNRGDLFPLGSVG
                     HTGWTGTSMWLDPATDTFVVLLTNRNHPDESGNVVALRGKVATIVAAAITDRRPEELR
                     KASEETASLAAIGAATSASKSTSAPSAPSSKVNLAAAVAAPARVVGQVRPGIDTLEEK
                     SFDVLKGLRVALLTNHTGLTRDGRSTIDVLNGEKAKAAGVSLLRLFSPEHGIRGALDE
                     KVSDSVDEKTGLPIRSLYGDTPESRRPRPEDLAGLDAVVVDLQDAGCRFYTYLTTLGY
                     VLEEAAKAKIKVVVLDRPNPIRADLAEGPPADADALTFVAYHPIPIRTGMTIGELAGL
                     FNAERKIGADLTVVKMAGYSRDLWYDETGLTWVNPSPNLRSVTEAALYPGVGLLETTN
                     VSVGRGTDTPFEQFGAPWMDGPRVAATLNARGIPGVRFTPVRFTPSSSVFKGESCSGV
                     RITVVNRDALNAVALGFHAATALRDLHPKEWKAERFNRLLVNAAALARFTRGETAGEI
                     TGAWAAGAMEFEKRRAAFLLY"
     gene            complement(9049..11049)
                     /locus_tag="PLB01_09415"
     CDS             complement(9049..11049)
                     /locus_tag="PLB01_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQQ77559.1"
                     /translation="MKGVRAAAVLAALLVAGGSSSAFSQQPGYSGHGAESVPAELVAK
                     YAAPPLDGAVSRRIQTMLDVRTPGLGVVSPDGSRLYFGWRITGTSQVFRLNAPKGFPV
                     QMTGGEDRTSVDAVTPDGKWLVLSRDVGGQENPGLYLQPADGGSLKTIQKTEKVQTFL
                     DFVSEDGKDLYFHANDVAADSYAIYRYDLATGVKTLVFGEKGLWAVADHAGSGAGLRL
                     LLVKATGSFSTEYAEYVPATKAFTPLLGAGEATEWDAAYGAQPGELLVRTNKFSDFRR
                     LYRWKKGGDATAKSFVEVLAPAGMDVAGFTVDLPRRHVYASINDGGYSRLRVLDAKTF
                     APMDIPLPKDADQVYAGSATPDGRFVTIGVETAQAPRTSYVWDWEKKTLTQWVLPSAP
                     EVDLSAFVSAKLMTYPAKDGTKIPMFVRFPKGCAPGENAASDPCPVIVEFHGGPEAQA
                     TPGFSTYAQMFVDAGFVYVEPNVRGSDGYGKAWLDADNGPRRLDVISDIDDAGKWIRA
                     NWGRNGKAPRVGVTGGSYGGYATLVAMTMFAGTYDAGAAEVGISNLETFLRNTAPYRR
                     ILRISEYGDPDKDADALRKLSPVTYLDRVKAPLLVIQGVNDPRVPAGEAIQVHDLLEK
                     RGVSAPLILFADEGHGASKRANQVLSIGHVIRFFEQNLKGTK"
     gene            complement(11194..12192)
                     /locus_tag="PLB01_09420"
     CDS             complement(11194..12192)
                     /locus_tag="PLB01_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HQQ77560.1"
                     /translation="MLLPLLLLQAAAAAPTPAALTVIPKHDELAYTFGGVAPRYRVKP
                     GTRIVSWTEDCFDGAVKTTSDLPSKVMTPGHDNPQTGPFFVEGAEPGDTLAIRILRLA
                     PARTWAVSAFGPGFGALVGTDRTAMLGPEFPETVWRYEVDAKRGVARTTSGDGKQGWE
                     VPLAPFLGCLGVAPSMGEARSTIVPGTFGGNMDCPEVRAGNTVYLGVNVPGGLLSFGD
                     GHFAMGDGEIMGAAVEGAMDVEIVVDLVKGKATPVPRIENADEVMFVGSGRPLEDAAR
                     MAFKAMVGWVREATGLSELDAYQFVSQFAKAPIVQLVDPEYTVLVKIEKKRLPKPR"
     gene            complement(12225..12575)
                     /locus_tag="PLB01_09425"
     CDS             complement(12225..12575)
                     /locus_tag="PLB01_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HQQ77561.1"
                     /translation="MTRLSPCPATPNCVLSTATDETHRIAPFPFVGDAAEALARVKAA
                     ALSFPRTRVAEEAQGYVRLTFKSALFGFVDDVEFEVDEAAKVIHVRSASRVGRSDLGV
                     NRKRVEAIRAKLGG"
BASE COUNT         1986 a         4286 c         4488 g         2010 t
ORIGIN      
        1 cgtcacgacg gcgagggcga cggccgccgc ggccggcgtg aacgcgccca tgcgtgtcgc
       61 cgcctcgccg acgagcggga aggcgacgat cgctgccgtc aggccgtgga cgtccgcgct
      121 cgcattggcc ccgcgcctgc cgtcgcggag cgcgaggacg acccaggcga gggcgtagac
      181 gaggccgagg agcgcgccca ccacgacggg cacgcggccc gcctccgtga gcgtccggag
      241 caggaacgcg ccgccgagga cgaggaagct gcgtccggcc agcgtgatgg cgttcgcgac
      301 gggagcgccc tcttccgcct gcgacgggac gggcccgccg ggaacggccg ctgcggcgat
      361 cgcgggggcg gcggggaccg cggtctcgac cgccgccggg cggacctgga tcgggcccgt
      421 ccggccctcg agcgccgcga gccgcgcgga gacgtccttc agcgcgctct cgagcagagc
      481 gacgcggccc tcgaggtcgg cccgtccgcc gtccatgcgc ggctccttcc cgtcaggcgg
      541 cggccggctc cgccttgggc tcgtgcggct cctcgaacgg aggaaggatc ttgacgagcg
      601 cccagaggat cacgaacggg aggatcatga ggacgatcgc cggaagcagc ccctccttcg
      661 tcgcgaaggc catcttgtac gtcgtgtacc cgaggaagct cttcgagaag agctggccgc
      721 cgatcacgac gttccagcgc atcgcgaaga tgcccatgag cgtcaggcag cctgcgaaca
      781 cgtagatccc ctttcgcgcc gactcgctga gcttcacgag ctgcgtgaag gcgaggagcc
      841 cgagcgggac gatggtgccg aagacgatct ggatcaggat ctgcgacgtg tagaggcgcg
      901 tgtggaccat gaagtcgagg gactggaacg actcgtccgc ctcgtagatc cggtggatga
      961 ggtccatgag ctcgatcgtg aagtcgatca ggaacgcgta gaacaggtag cgggcgatcg
     1021 tgtcgacgca cggcatgtcg atcgggacac ccttgaggac gcacacggcc atgtacatga
     1081 gcatgacgag cgcgattccc gagacgatcg ccgagaagag aaagacgatc ggcatgacgg
     1141 gcgtcgacca ccaggggttc gccttgatcg acccgaagat gaacccgacg tagccgtgga
     1201 ggaggaacgc cgacgggatg ccgatgatcg tgatgaagcg cccgaccttg tcgtcgatcc
     1261 tgagcgactc ctcgctgatg ttgtcgacgc cgaggctcag ggccttgtag agcgtcttct
     1321 tccaacccgt ctccgaccgc gacatcagga cgatgtcctt gcggtagtcg agccagatct
     1381 cgaggacgag gacggccatg aggtaccaca ggtaaacgaa gccgaacatc gccatcgcgg
     1441 acgaggtgtg cggcgtgagg tacatctcga acgagcgctc ggggtggccg aggtggagct
     1501 gaaggggcag cggcgcgacg atgaggaacg cgagggccga gaggagcgcg agacggtacg
     1561 tcggcttgac ggccgcgacg ttgaagacgc gctcgagcga ggcgaggatg aacgcccccg
     1621 cgacgaggcc cgtcgtgaac gggtagagaa cgatcaggat gctccactgg agctcgacct
     1681 cgttggggta catgtacccc tggacggcgt ggagaaggct ttcttccatg ttcctacctc
     1741 accgacccgt cgagggcgtt gtagaagagc ttggagcccg tcgccatctg cggcttcagg
     1801 acgtgcacct tgtgcgtcct caggaactcg tggacgggat ccctcgggtc cttgaggtcg
     1861 acgagctgcc gggcgcccgt cgggcagttc tcgcagcagg ccgtcgtgag gcctttcgtg
     1921 atccggtggt aacagaggtt gcacttgtcg accgtctccg tgcgcgggtc gatgaaccgg
     1981 cagccgtacg gacaggcctg gacgcagtag cggcagccca ggcagtacgt cttgtcgacg
     2041 agaacggcgc cgtcgggggc ctcgaacgtc gcgccgaccg ggcagacctg cgtgcagggc
     2101 gagtgcgcgc actggttgca gagcttgggg acgaagaagc tcttcgcctt gtcgccggac
     2161 gcgaatttct cggggaagcc gttgtacccg ccgttcggcg agtcgacctc cggatgttcc
     2221 gagagcggct cgacgtggta gcgctcgacc caggtccgga agtagaaagg ctcgaggggg
     2281 acgttgttct cggtcttgca ggcgcgcacg cacccgccgc agccgatgca cttgtcgatg
     2341 tcgacgatca ttccccagaa gtggtcctgc atccggtagg cggactcctg ggcttccgtc
     2401 ttgcctgcgg cgagggcgtc ccagtacgcg gcggcgggcg ccgtgaggac gagcaccttg
     2461 cccgcggtcg cgaggaattc gcggcggtcc tggctcattt cttcggctcc ttgtccggcg
     2521 cgtgctggtc gtgacaggtc aggcaggggg cgccgtccat gtgcgatttc gggtcgatct
     2581 gcgggaactt cgcgggcttg gcgacgttca cgaggtggca cgtctgacag agcgtcttcg
     2641 cgtccggctt cgccggcttg agagccgacg ggtccgccgc gtgcttggcg agcgcgccgt
     2701 ggcaggactc gcagccgacg ttcttgtgct tgccgccctt gacgcggtcc ccttcgtccg
     2761 tgtggcactc gacgcaggcc gcgcggcccg cgtaggcgac gggccgcgcc gcgacgtcgg
     2821 cgagcgcgcc cgcgcggtag tgcccgtagt ccccgaagcc cttgggcacg aggatcgcgc
     2881 gggcgatcac gaagaccacg atccccgcga gaaacagccc cgcgacccga accaggtgtt
     2941 ccttgtcctt gaagagcctg ttcatcgacg atccacctcg cggatcttga gatagagaag
     3001 aacgccgaag accgagatga ggacggtcgc caggaggagg ccgttccgcc ggaaccggta
     3061 ctccctccag acgttcgcgg tcccggcctt cagggcatcg agggccgctt tcgtcgcatc
     3121 cgagttgcgc gtgaagtcgc tgcgatcgaa cgtgtggatc ccgaggcgcg tcttgcgcag
     3181 cgcgtcgttc acggcgctcg actggaagcg cagctcgtcc acgtcgaacc cgcgctcgag
     3241 gagccggtcg acgtcgtgat tgagcgtctc gtgcgacgtc cggagggacg tgatcgacgc
     3301 gtagaagtac ttcgccgtct cgtcgcactt cggctggccg tactttgcgt ggcacgcgtg
     3361 gcagaggccc ttcgggtcgt cgctcagcat ctcgtccgtc ggacgctgga tgtcgtgctt
     3421 gccgtggcac gtttcgcact cgggccagcc gttcttctcg aacgacttct tgtgcgggct
     3481 gccgtcgaag aggctgccgt tcgcggcgtg gcagtttcgg cacacctgcg acacgaacgc
     3541 gacctgcggc ggcagcgccg cgtggttgcc gtggcagctg ttgcaggccg gggcgccgac
     3601 gtcgtgcttc acgaggaggg cgatcccgtg gacgctcttc ttgtactgct cgagctggtt
     3661 cgtcgggagc ggcgtcttgc cgtcgtcgag cacgtagccc ttcatgtacg cggcatccgc
     3721 gtggcacttg ccgcaggtcg acgggatgtt ctcgcggtag acgagggacg tcgtgctgtc
     3781 gggcggccgg atcccgtgca cgccgtggca gctcacgcac tgcgcggcct tggagtcctt
     3841 cttctcgaga aggagctggc cgtgcttcga cgtgcggtac ttcagaagct ggtcgacggg
     3901 cagggacggg ttgtacgccc gcatgaactc gctcgacgag tggcagcgcg cgcagaactc
     3961 cgggatccac gagcggtcct tgtgacggac ctcggacgac ggccaccgtt tgccgatctc
     4021 ggccgtcatc tccttgtcgg tcggcttgcc gccgtggcag cccgcgcaca tgaggccgcc
     4081 gcggacgtgg gcgtcgctca cgacctcgcg gatctcgagg tgggcgagcg gatccccctt
     4141 ctcgcgcttg cggaagagga tctcgtccgg caggtcgggg ttcgagtggc agaggaagca
     4201 gttgtccccg aggatgagct cgactttccg gtcgtcgata tcgagcgcga gcagcgccgc
     4261 gaggtgggtc gcgcccgaga gtgtcccggc tttcgcggcc gcgaagaaga gctcctgcgc
     4321 gtgcgtcggg agcgccttga agcgcgcgcg ggccttttcg tcgaggaccg gcacgccctt
     4381 ctcggccgct tcgaacatct tcttgaagtc cgcgggccag gccgtctcgc cgacgtgccc
     4441 ggcgggtgcg gccgccgcgg ccgcgggcgc cgcgcccttt tcgtcggcgc gcagctgcgg
     4501 ggcgacgagc gccgccagca cgaggacgga ggccgccacg gcgccggaac ccttcacgcg
     4561 gcggaagatc gtcatcgccg cgagcgccgc gagcgcgatg ccgcccgaga cgagccagga
     4621 gaggtgcgcg aagccgttca gagcggcctg caggaggacc gcgatcgtga agtcgaagtc
     4681 cgaatgcttc cacttcaggc ggcgcagcac cgtcacggcg atcccgatcc cgaggatcca
     4741 cagggcggcc gtgcgtgcga tcgtgttcgc gagaacggga tcggcggtgg ggtcctttcc
     4801 cttgggaacc gtcacgccga cgtcccacgc cttcacggtc aggaagccga ggaagaggag
     4861 gcccgcgacg ccgaggtccg tgaagagcgg gctcggcttg cccttcgccg cgttgcggtc
     4921 gaggaggggg atgaagaacg cgatgccgcc gaggatgctc gcgaggagga tgcaggcctg
     4981 cggcccttcc atgcccgcga tgtgcgccgg gaactccttg agcatctggt agacccacag
     5041 gaagtaccac tcgggcttga tgccggggta cgcgggcttg agcgggttcg ccttctcgcc
     5101 gagctcccac tccatgccgg ggatgccggg accgaacgga aggatgacgg cgaggagcgc
     5161 gaggacgttc aggacgatga cccacaggag caggtcgcgg agcgcgaagt tcgggaagaa
     5221 cggcattccc ctcttcttct cgaggggctc gtggccggtc tccatcggct cggccatgcc
     5281 ctggcgctgg atgaagagaa gatgcgctcc gatgaggccc gcgatcacga gcggcaggat
     5341 gcagacgtgc agcgcgaaga accggtagag cgtctggacg gaaacctcgt cgccgccgcg
     5401 caggacgcga aggagccacg ggcccacgag gggaatcgcc ttgagcgagt ccgtcccgac
     5461 cgccgtcgcg aagtagctca gctcgttcca cgggaggagg taccccgaga agccgaaccc
     5521 gaggacgagc ccgagcatcg cgaaccccgt gtaccacgtg agttcccgcg gcttgcggta
     5581 ggccttgacg aacaggacgc tgaacaggtg cgcgaggacg gcgaggctca tgaggtgcgc
     5641 cgaccagcag tggatcgagc ggatgagcca gccgaacggg acgcgcccga cgaggtgctt
     5701 catcgactcg taggacgtgt tctcccccac ctggtagttc atgaggagga ggatcccggt
     5761 gccgatctgc acgacgaaca ggaagaggga caggccgccg aggtagtacc agacgatcga
     5821 gtgcgcgacg gggacttcct tgtgcgaaag gaactcgacg agaccggaga ggtggaagcg
     5881 ctcgtcgacc gcctcccagg cgcgggtcgc gagattgcgg gcgggcgtcg cgctcacgcg
     5941 gcacttccct tcgagaccgt cacggcgccc ttgaggtcgc cttcgacggt gaacgcggcg
     6001 agcggccgcg gcggcgggcc cgagatcacg cgcccgtcca ttgcgaactt cccgttgtgg
     6061 caggcgcacc agacgattcc gtcggccggc acgaactgca ccgtgcagcc ctcgtgcgtg
     6121 cacttggccg acatcgcgcg gagcttctgg tccttgtcct tgaggaggag gacacgttcg
     6181 ctcccgaggc gtgcgatcag gaagccgttg gtcccgagct cctgcttctg tgcgtccgtc
     6241 agggtgacct tgttcgcgcc cccggcatcc ttggccggcg tcaggtagcg cagaaccgga
     6301 tagacgaccg cgagccccca tgcgacgaag ctgcttccga gcagcacgtc gatccagcgg
     6361 cggcgcgaga acgcggcatc cggctcacga gacaggctgt tttccaccgt tcctcccgat
     6421 cccctgtgga cgacgtcgaa tgccccattc ccgcaggcgt gttcttcgac gcgcgcgcgg
     6481 gagcctgatc tcgttgttgg cgccgatgct ggtcccggac ccggatttcg gccatgatgc
     6541 gattgcttca aaagggggcc gccgggcagg cggctagact cagacgcatg ctcttcgcgc
     6601 ccgtcctggc cgccgtcgcc gccctttctg cgcccgtggc acccgcgtcc gtcggcatgt
     6661 ccggcgggcg cctggcggat ctggatgccg ccattgcgga ttcgatcgcg aagaaggagt
     6721 gcccgggcgc cgtcgtcctc gtcgggcgcc acggcaaggt cgtcttccgc aaggcctacg
     6781 gcaaccgggc cgtcgtgccc gccgtcgagc cgatgaccgt cgacacggtc ttcgacctgg
     6841 cgtcgctcac gaaggtcgtc gcgacggcga cgagcgtcat gacgctcgtc gaggacggaa
     6901 agatccggct ccaggaccgc gtcgcgaagc tcattcccga gttcgcggcg ggcggcggcg
     6961 cccgcgacca ggtgacggtc gaggagctcc tcacgcaccg ggcggggcta gtcgccgacg
     7021 acccaatggc tctctatgtc ggaacgccgc aagagatctt tgaaaggaaa tacaggcagc
     7081 cgctcgtgaa tgcgccggga gcgaagttcg tctactccga cgcaggttac gaggtccttg
     7141 gagagattct tagaaaggtc agcgggctgc gcctcgacga gtacgcggcg aaacgcgtgt
     7201 tcgagccgct cggaatgaag gagaccggct ttctgccgct ctccccctct tcaccttcca
     7261 ggaaatcttc tccttctctt cccctctccc gcatcgcgcc caccgagagg atcaacggcg
     7321 aaattcgccg cggcgccgtg cacgacccgc gcgcgtacgc gctcggcggc gtcgcggggc
     7381 acgcgggcct cttctcgacg gcggacgacc tcgcgaagtt ctgcgccgcg atgctcaggg
     7441 gcggcggcgg cgtgctctcg ccggccggcg tggcgtcgat gctgcggccg cgtttctacg
     7501 gcgacggggt cctgcgcggc ctgggctggg acgtcggctc gagctactcc acgaaccgcg
     7561 gcgacctctt cccgctcggc tccgtcgggc atacgggctg gacgggcacc tcgatgtggc
     7621 tcgacccggc gacggacacg ttcgtggttc ttctgacgaa ccgcaaccac cccgacgagt
     7681 cgggcaacgt cgtcgcactg cgcggaaagg tcgcgacgat cgtcgcggcg gcgatcacgg
     7741 acagaagacc ggaagagctt cgaaaggctt cggaggagac cgcgtccctt gcggccattg
     7801 gcgccgcgac gtcggcgtcg aagagcacga gcgcgccttc ggcgccttct tcgaaggtga
     7861 atttggctgc ggctgtcgcc gcgccggcgc gcgtcgtggg gcaggtccgg ccgggcatcg
     7921 acacactcga agaaaaatcc ttcgatgtcc tcaagggcct gagggtcgcc ctgctgacga
     7981 accacacggg cctgacgcgc gacggccgct cgacgatcga cgttttgaat ggggagaagg
     8041 cgaaggccgc cggcgtcagc cttctaagac tcttctctcc cgaacatggc attcgagggg
     8101 cgctcgacga gaaggtctcc gactccgtcg acgagaagac ggggctgccg attcgctcgc
     8161 tctacggcga cacgccggag tcgcggcggc cgcggccgga ggatctcgcg gggctcgacg
     8221 ccgtcgtggt ggacctgcag gatgcgggct gccgcttcta cacgtatctc acgacgctcg
     8281 gctacgtcct cgaggaggcg gccaaagcga agatcaaggt cgtcgtcctc gaccggccga
     8341 acccgatccg ggcggacctg gcggaaggcc cgcccgccga cgccgatgcg ctcacgttcg
     8401 ttgcgtatca ccccatcccg atccggacgg ggatgaccat cggcgagctg gccggcctct
     8461 tcaacgcgga gcggaagatc ggcgcggacc tgaccgtcgt gaagatggcg ggttattccc
     8521 gcgacctctg gtacgacgag acgggcctga cgtgggtgaa cccgtcgccg aacctgcgct
     8581 cggtgacgga ggccgcgctc tatccgggcg tcgggctcct cgagacgacg aacgtctccg
     8641 tgggccgcgg gaccgacacg cccttcgagc agttcggcgc gccgtggatg gacgggccgc
     8701 gcgtcgcggc cacgctgaac gcgcgcggga tcccgggcgt gcgcttcacg cccgtgcgct
     8761 tcacgccgtc ctcgtccgtc ttcaagggcg agagctgctc gggcgtgcgg atcacggtcg
     8821 tgaaccgcga cgcgctgaac gccgttgcgc tcggcttcca cgccgccacg gccctccgcg
     8881 accttcaccc gaaggagtgg aaggccgagc gtttcaaccg gctcctcgtg aacgccgccg
     8941 ccctcgcccg cttcacgcgc ggcgagacgg cgggcgagat caccggagcg tgggcggccg
     9001 gcgcgatgga gttcgagaag cgccgggccg ccttcctcct ctactgacct acttcgtccc
     9061 cttcaggttc tgctcgaaga accggatcac gtgcccgatc gagagcacct ggttcgcccg
     9121 cttgctcgcg ccgtgcccct cgtccgcgaa gaggatgagc ggcgccgaaa cgccgcgctt
     9181 ctcgagaagg tcgtggacct ggatcgcctc gccggccggc acgcgcgggt cgttcacgcc
     9241 ctggatcacg aggagcggcg ccttcacgcg gtcgaggtac gtcacggggc tgagcttcct
     9301 gagggcgtcg gcatccttgt cggggtctcc gtactccgag atgcgcagga tccgccggta
     9361 cggcgccgtg tttcgcagga aggtctcgag gttcgagatc ccgacctctg ccgcgcccgc
     9421 gtcgtacgtc cccgcgaaca tcgtcatcgc gacgagcgtc gcgtacccgc cgtagctgcc
     9481 gcccgtgacg cccacgcgcg gcgccttccc gttgcggccc cagtttgcgc ggatccactt
     9541 ccccgcgtcg tcgatgtccg agatcacgtc gaggcgcctc ggcccgttgt cggcgtccag
     9601 ccacgccttg ccgtagccgt cgctgccgcg cacgttcggc tcgacgtaga cgaagcccgc
     9661 gtcgacgaac atctgcgcgt acgtcgagaa gccgggcgtc gcctgcgcct cgggcccgcc
     9721 gtggaactcg acgatcacgg ggcacgggtc cgaggccgcg ttctcccccg gcgcgcagcc
     9781 cttcgggaag cgcacgaaca tcgggatctt cgtgccgtcc ttcgccggat acgtcatgag
     9841 cttcgcggag acgaacgccg agagatccac ttcgggcgcc gacgggagga cccactgagt
     9901 cagcgtcttc ttctcccagt cccagacgta gctcgtgcgc ggggcctggg ccgtctcgac
     9961 gccgatcgtc acgaagcgtc cgtcgggagt cgccgacccc gcgtacacct ggtcggcgtc
    10021 cttcgggagc gggatgtcca tcggggcgaa ggtcttcgcg tcgagcactc tgaggcgcga
    10081 gtaccccccg tcgttgatcg aggcgtagac gtggcggcgc gggaggtcga ccgtgaaccc
    10141 ggcgacgtcc atcccggccg gcgcgagaac ctcgacgaag ctcttcgcgg tcgcgtcgcc
    10201 gcccttcttc cagcggtaga ggcgccggaa gtccgagaac ttgttcgtcc tgacgaggag
    10261 ctcgcccggc tgggccccgt acgccgcgtc ccactccgtc gcttcgcccg cgccgagcag
    10321 cggcgtgaac gccttcgtcg cgggcacgta ctcggcgtac tccgtcgaga acgagcccgt
    10381 cgccttgacg aggaggaggc gcaggccggc tcccgacccc gcgtggtccg cgaccgccca
    10441 gaggcccttt tccccgaaga cgagcgtttt cacgcccgtg gcgaggtcgt accggtagat
    10501 cgcgtaactg tcggctgcga cgtcgttcgc gtggaagtag aggtccttcc cgtcttccga
    10561 cacgaagtcg aggaacgtct gcaccttttc cgtcttctgg atcgtcttca gcgagccgcc
    10621 gtccgcgggc tggaggtaga gcccggggtt ctcctgcccg ccgacgtcac gcgagaggac
    10681 gagccacttg ccgtccgggg tgacggcgtc gaccgacgtc cggtcctcgc cgcccgtcat
    10741 ctggacggga aagcccttcg gcgcgttcag gcggaagacc tgcgacgtcc ccgtgatgcg
    10801 ccagccgaag tagaggcgcg acccgtcggg cgaaacgacg ccgaggccgg gcgtgcggac
    10861 gtcgagcatc gtctggatgc gccgggagac cgccccgtcc agcggcggcg ccgcgtactt
    10921 cgcgacgagc tcggcaggga cgctctcggc gccgtggccc gaatagccgg gctgctggga
    10981 gaaggcggaa gacgaacccc cggccacaag aagcgcggcg agaacagccg cagcgcgaac
    11041 acccttcata gacatctcct tagaaggtga atacgccggt caaggcggtg gggttttacc
    11101 gcgtccgcgc cggcccgcgc cgccgggcgc ctgcacccct gccctcttac ccttctaaga
    11161 aatcccgggg ccgcgcgaag cgcggctccc ctgtcatcgc ggcttcggca gccgcttctt
    11221 ctcgatcttc acgaggaccg tgtactcggg gtccacgagc tggacgatcg gggctttcgc
    11281 gaattgggac acgaactggt acgcgtcgag ctcggagagg ccggtcgcct cgcgcaccca
    11341 acccaccatc gccttgaacg ccatgcgcgc ggcatcctcc agcgggcgcc ccgagcccac
    11401 gaacatgacc tcgtcggcgt tctcgattcg cgggacgggc gtcgccttcc ccttcacgag
    11461 gtccacgacg atctcgacgt ccatcgcgcc ctcgacggcc gcgcccatga tctcgccgtc
    11521 gcccatcgca aagtggccgt cgccgaacga caggaggccg ccggggacgt tcacgccgag
    11581 gtagaccgtg tttcccgcgc ggacctcggg gcagtccatg ttcccgccga acgtcccggg
    11641 cacgatcgtg gagcgcgcct cgcccatcga gggcgcgacg ccgaggcagc cgaggaacgg
    11701 cgcgagcggc acctcccacc cctgcttccc gtctccggac gtcgtgcgtg cgacgccccg
    11761 cttcgcgtcg acctcgtacc gccagacggt ctccggaaac tccggcccga gcatcgccgt
    11821 gcggtccgtc cccacgagcg cgccgaagcc ggggccgaaa gccgacacgg cccacgtccg
    11881 ggcgggggcg agccggagga tgcgtatcgc gagcgtgtcg cccggctcgg cgccctcgac
    11941 gaagaaaggc cccgtctgcg ggttgtcgtg gcccggtgtc atcaccttcg acggcaggtc
    12001 ggaggtcgtt ttcacggcgc cgtcgaagca gtcctccgtc cacgacacga tccgcgtccc
    12061 gggcttgacc cggtagcgcg gggccacacc gccgaacgta taggcgagct cgtcgtgctt
    12121 cgggatcacg gtgagggcgg cgggggtcgg agccgcggcc gcggcctgga ggaggagcag
    12181 ggggagcagc atggcgcgga cgctatcagc cccgcgtgca gccgtcagcc cccgagcttc
    12241 gcccggatcg cctcgacacg cttgcggttg acgccgaggt ccgaacgccc gacgcgcgag
    12301 gccgagcgaa cgtggatcac cttcgcggcc tcgtccacct cgaattccac gtcgtccacg
    12361 aagccgaaaa gcgcgctttt gaacgtcagg cggacgtagc cctgagcctc ctccgcgacc
    12421 ctcgtccggg ggaaggagag cgccgccgcc ttgacgcggg cgagggcctc ggctgcgtcg
    12481 cccacgaacg ggaacggcgc gatgcggtgc gtctcgtccg tcgccgtgga gaggacgcag
    12541 ttcggcgtcg ccgggcaggg tgagagacga gtcataggcg gggctcctgt gcaggccgat
    12601 gcgaagagag cggcaaccgc gagagctgcg gggcggagtc cggtgcgcat gcccactcta
    12661 cgccggccgg gcgagggtcg gtttctcctc ggcgccggcg gccggcgcct gcggggccgg
    12721 ctgcgcgagc gccggcgccg gcttcggcgt cacgcggcgg aacaccgagc
//
LOCUS       DAOMFY010000094        12736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1522_length_12736_cov_7.633231, whole genome shotgun sequence.
ACCESSION   DAOMFY010000094 DAOMFY010000000
VERSION     DAOMFY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_12736_cov_7.633231"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PLB01_09430"
     CDS             <1..1202
                     /locus_tag="PLB01_09430"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQQ77562.1"
                     /translation="TALIVFLWLVLPVAAPPRPFEPQGPLGALLALERRFDSPACAFP
                     SFHVAWAFLAAPVWAARLGRARSLAWAWAWAITLSCVTTGMHTVADVVSGLVAAALVL
                     RLPAAWEALRAASERLANSWREWDFGAVRVINHGGWAALGTLAGVGLAASLAGESAVT
                     AALLVAFSGLAGAGLWAQFIEGSPRLLRPYGYYGGVIGVVLGCLAARLTLGVDAWLLL
                     GAFAVAAPWIQAAGRVRCLVQGCCHGAPAPPEVGIRYVHVRSRVCRLSDLGGVPLHPT
                     PLYSILWNVVPALLGARLWAVRAPLPVIVGVYLLLAGIGRFVEESRRGEPQTPVFARL
                     RLYQWMAAGTAAAGAAVTGLRATVVTPAPQPSAAALGTAVLFAAATWFALGVDFPRSN
                     RRFARLV"
     gene            1199..1903
                     /locus_tag="PLB01_09435"
     CDS             1199..1903
                     /locus_tag="PLB01_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77563.1"
                     /translation="MRTDFLLRIHALLLVAFVAAASAAAQEPPKKGKAFEPPPEPERR
                     WELTLGGAGGFASSSRDVDAAFGAAGYTPSSSGDFLSATVFPSIRFRLGERAAVGVSF
                     SSTKLGSTTGTGFGTSVSIGRSSTDVALVAFWRPFPGLRVGAGPAWYRLTAAPDVGES
                     LEVSKLGWIAEAGLAYPDRGAWYVDLAVQYRGTGDADFGTYVPSAKGPIAPAPISLDG
                     IGCEHGAFLAGIGFRF"
     gene            complement(1911..2246)
                     /locus_tag="PLB01_09440"
     CDS             complement(1911..2246)
                     /locus_tag="PLB01_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQQ77564.1"
                     /translation="MAQHETSASTSAPAPHVMPLSTYFLVFAALMVGTAATVGAAYVD
                     LGWANNAVALAIAIAKATLVVLYFMHVKENTRLIPAVIFSGLFMLLILFVNLLADYGT
                     RGWVGVEGR"
     gene            complement(2246..2902)
                     /locus_tag="PLB01_09445"
     CDS             complement(2246..2902)
                     /locus_tag="PLB01_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HQQ77565.1"
                     /translation="MSVESHGPSDLAHHFDSYEQQRESATLGMWTFLVTEILFFGGLF
                     AAYSLYRSLYPEAFRAASHTLDIRLGAFNTAVLIGSSLTMALAVRGGQTGKPKTTFAM
                     LLGTLLLGSTFLAVKAVEYADKFTHHHVPGPSFHFEGPDAPHAQLFFSLYFGMTGLHA
                     LHMVIGAGILVFMLRPALRGAWTPRNHNFLEGFGLYWHFVDLVWIFLFPLLYLIGRHH
                     "
     gene            complement(2892..4541)
                     /gene="ctaD"
                     /locus_tag="PLB01_09450"
     CDS             complement(2892..4541)
                     /gene="ctaD"
                     /locus_tag="PLB01_09450"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HQQ77566.1"
                     /translation="MTSSPNPVPAEFPRTHYLNAAYGLKSWLWTRDHKRIALLYLVSV
                     SVFFALGGLMAVLIRMELATPEGDFFQPDVYNRLFTMHGVLMIFFFLIPGIPAVLGNF
                     LIPLMIGAKDLAFPRINLLSWYLYTIGGAFTLWALLHGGVDTGWTFYTPLSTNYVNTS
                     VLLVGLGIFIAGFSSILTGLNFVVTIHRMRAPGLTWFRLPLFVWGHYATSIIQILGTP
                     VVAIAILMVAAERVLHIGFFDPKLGGDPVLFQHLFWFYSHPAVYIMILPAMGVISELV
                     AAFSRKPIFGYKFVAFASIGIAILGFLVWGHHMFVAGQSIYAGVVFSVLSMLVAVPSA
                     VKVFNWTATLYKGSISWDTPMLYALGFIGLFTIGGLTGVMLATLGIDAHVHDTYFIIA
                     HFHYIMVGGAIMGYLGGLHFWWPKITGRLYPVFWSKLSALVIFIGFNVTFFPQFILGY
                     LGMPRRYHTYPPEFQVLNVMSSAGATILGIGYLMPLVYFVWSMRYGPRATANPWGATG
                     LEWQTTSPPPVENFTYPPVVTAPPYTYEAQPAAGKGAARVG"
     gene            complement(4538..5518)
                     /gene="coxB"
                     /locus_tag="PLB01_09455"
     CDS             complement(4538..5518)
                     /gene="coxB"
                     /locus_tag="PLB01_09455"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQQ77567.1"
                     /translation="MPNLPDSPLFPVQGSDIARSVDHLMLFALGGLVVFSTLIAVLVL
                     VFMVKYRRKHEGEIGEDVYETNPKSHVLELVWSVIPLGLLLVLFGWGTKVFFDQNRPP
                     AGAAQYWVTGRQWMWKIQHPSGRREINELHVPVGQAVQLRMISEDVIHDFFVPAFRIH
                     VDVLPSRYTTYWFKATKTGEFHLFCGQYCGVEHSKMVGKIVVMEPRAYEAWLAGEPAA
                     GAPAAAASGEQLFVAKACNTCHRPDSSARAPVLNGLFGTQVTLQDGAKVTADENYVRE
                     SILNPGAKIVQGYQPVMPTFKGQVTEEELIQLVNYVKTLKAAEGAAEARK"
     gene            complement(5518..6348)
                     /locus_tag="PLB01_09460"
     CDS             complement(5518..6348)
                     /locus_tag="PLB01_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQQ77568.1"
                     /translation="MTRSAPLALALALALSGELRATAQGAPPAEAPLPAPPLKDIGFD
                     QKIGDQVPLDLAFRDEAGKTVHLRDYLGKKPVVLSLVYFDCPMLCGMTTDGLVRSLRA
                     LRMSVGTEFDVLSVSFDPRETSEMASAKKGPVMRQYGRKGGAEGWHFLTGDKASVDAL
                     TKAVGFRYVWDAEQKQYAHATGVLVLTPQGRIARYFFGIEYPAKDLRLGLIEASEEKL
                     GSVVDQLLLLCYHYDPKVGRYTATVRNFLRGGAVLTIVAVAGLVLTLLKREKRRTGAA
                     "
     gene            complement(6345..6740)
                     /locus_tag="PLB01_09465"
     CDS             complement(6345..6740)
                     /locus_tag="PLB01_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77569.1"
                     /translation="MAENRGVDSELNLKAVAVSVAVLAAVCIAAFALMWGVAVWQKKR
                     LVAQDPPPPVLAEARVKSLPAGVLLQDDPEKDLKVLRAREDAALAGWSWTDASKTRAR
                     IPADRALEIVAAKGFPPRTAPPAAPEVKK"
     gene            complement(6733..7944)
                     /locus_tag="PLB01_09470"
     CDS             complement(6733..7944)
                     /locus_tag="PLB01_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77570.1"
                     /translation="MTEHHPLKLAAADWAPPAADIARVGRRALVVGAVFAAASAAGFV
                     ANPVQFYRSYLVAFGLVLGVALGSLAIQALQHMTGGAWGLVIRRILEASSRTLPLVAL
                     FAVPLFFGLRHVYPWADPACVATDEILHHRAAYMNPAGMALRLALCFAVWAAFALALS
                     RLSKRQDEAPAAGLERRMQLVAAPALAIYCLLISVASIDLLMSTDAHWFSAIYGIYVV
                     GGQAISGMAFTLLVALFLAKRAPFRAAVQQRHVHDWGKLLFAFTMLWAYFSVSQLIII
                     WSGDLPEEITFYKARVTGGWMAVSLALALFQFVVPFVFLLSANFKKHLGRLSAIAALL
                     LLMRGVDLFWLVAPAWHPEGLSIHWLDAALPAALFGLWLAAFARELAKRPLLPVNDPA
                     LPAAMEVHAHG"
     gene            complement(7941..8663)
                     /locus_tag="PLB01_09475"
     CDS             complement(7941..8663)
                     /locus_tag="PLB01_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQQ77571.1"
                     /translation="MRRTLLLPLLALALAGCRNDMHNQAKTKPLRESPFFADHSSARP
                     LPEGVVARGFLRADAAIYRGVAEDGKFVLDIPVPVTRPLLLRGRERFDIFCSPCHGRT
                     GDGQGMIVQRGFKSPSTFHVDRLRNERAGYFFDVMTNGFGQMSSYASQITPEDRWAIV
                     AWMKTLQASQNMPKEFLAKDDEGKLEAAAAGGGTASPAQKSFLEGESGAAAAHPAPGA
                     APATSAPYSPSKKSSSEGSEAR"
     gene            complement(8663..9277)
                     /locus_tag="PLB01_09480"
     CDS             complement(8663..9277)
                     /locus_tag="PLB01_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HQQ77572.1"
                     /translation="MSLLSRLFPRHVAPGVGKPGPREGGGELPPHLYGVIAEFAGSPA
                     LVQAVHRARAEGYVVMEAYTPYPIEELNDAIGHGPSRVPLLVLIGGMLGFLGGFFLQY
                     WTAVVDYPLNVGGRPLNSWVAFIPITFECTILGAGIAAVLGMLVLNGLPMPYHPVFGV
                     DRFQLASRDKYFLMILARDPKFRAGDTFRFMETLGAVHVQDVED"
     gene            complement(9274..10647)
                     /gene="nrfD"
                     /locus_tag="PLB01_09485"
     CDS             complement(9274..10647)
                     /gene="nrfD"
                     /locus_tag="PLB01_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQQ77573.1"
                     /translation="MSAIPERPPVIAPGYTPATVTDKISAVVLNKTPKWWFLGFAVSF
                     VALQLLLMSMAKLLLLGVGVWGINIPVGWGFAIVNFVWWIGIGHAGTLISAILLLLRQ
                     SWRTSINRFAEAMTLFAVAAAGQFVLFHTGRQWLAAYWLFPYPNTMGLWPQFRSPLMW
                     DVFAVSTYATISALFWFVGLIPDLATLRDRSKSWFGKVAYGMLAMGWRGSARHWHNYE
                     VASLLLAGLATPLVVSVHTVVSFDFSVGIIPGWHATIFPPYFVAGAIYSGFAMVMTLS
                     IPLRKLFGLEDFITMRHLQNMAKIMLATGLIVAYGYAIEIFMAWYSGNPFEAFMIKNR
                     ILGPYGWIWFLLILCNILTPQLLWLRKVRNSVATLFVISIVVNIGMWFERFVIVVTSL
                     HRDFLPSSWDMYVPTRWDWAFYIGSLGLFFSLMFLFIRFLPVISIFEMRTIATGDPAD
                     KAPEVKA"
     gene            complement(10644..>12736)
                     /locus_tag="PLB01_09490"
     CDS             complement(10644..>12736)
                     /locus_tag="PLB01_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ77574.1"
                     /translation="IPFLLTNKVDVLVASLAVTPERAKQVQFSRPYSAATIVLYGSKK
                     IPIKAAADLKGAGARGLVVAGDYAPAEVHALVHAINGALGAIGTTVLLTGPVEAEPVD
                     QLASIRDLAADLKAGKVDTLLILGGNPVFDAPADLGFADAVQKAAFRVHLSLYDDETS
                     AYCTWQLNEAHPLEAWTDARAFDGTISIAQPLVEPLYGGKSAHEVLAAVLGQPSAKGG
                     DLVKAAWQKQRPAGFDAFWRKALHDGLVEGTALAPLALAPKAPAASPRATPVPAGSFE
                     LVLRPDPGLFDGRFANSGWLQEMPKPLTKLVWDNVAHLSPKSAEGLGLKNEDVVEIEA
                     NERKIELPVWILPGQPDGVVTVHLGFGRTRAGRVGNGVGVNAYALRTSASLWNATAAL
                     RKTGRAVELASTQQHFNMEGRHLVRTGTYETYRKDPEFAKKMEEVPEISLYPDWKYEQ
                     HAWGLAVNLSSCTGCSACVVACQAENNIPTVGKDQVRRGREMHWLRIDRYYEGTPEDP
                     AAHNQPVMCMHCEKAPCEVVCPVAATTHSEEGINEMTYNRCIGTRYCSNNCPYKVRRF
                     NFYDYNKDKHLPVLTLLANPNVTVRQRGVMEKCNYCIQRVNRARIAAEKENRRVRDGE
                     VVTACQQACPTEALVFGDLNDPTSKAAKLRKDPLEYPLLAELNTRPRTTYLAKLTNPN
                     PKLGGAPAATEHHG"
BASE COUNT         2044 a         4043 c         4752 g         1897 t
ORIGIN      
        1 cgacggcgct gatcgtgttc ctgtggctcg tgctgccggt cgcggcgccg ccgcggccgt
       61 tcgagccgca ggggccgctc ggcgcgctcc ttgcgctgga acgccgcttc gactcgccgg
      121 cctgtgcgtt cccgtccttc cacgtcgcct gggccttcct cgcggcgccc gtctgggcgg
      181 cgcggctcgg gcgcgcgcgc tccctcgcgt gggcctgggc gtgggcgatc acgctcagct
      241 gcgtcacgac cgggatgcac accgtggccg acgtcgtctc cggcctcgtt gccgcggccc
      301 tcgtcctgag gctgccggcc gcgtgggaag cgctgcgggc cgcctccgag cgcctcgcga
      361 actcgtggcg cgagtgggac ttcggcgcgg tgcgcgtcat caaccacggc ggctgggccg
      421 ctctggggac tctggccggc gtcggccttg ccgcgtctct cgcgggggag agcgccgtga
      481 ccgcggccct tctcgtcgcg ttctcgggcc tcgcgggcgc gggactgtgg gcgcagttca
      541 tcgagggctc gccgcgcctc ctccggccct acgggtacta cgggggcgtc atcggcgtcg
      601 tgctcggatg cctcgcggcg cggctgacgc tcggcgtcga cgcctggctg ctcctcggag
      661 cgttcgccgt cgcggcgccg tggatccagg ccgctggccg cgtgcgttgc ctcgtacagg
      721 gctgctgcca cggcgcgccc gcgccgccgg aggtcggcat ccggtacgtg cacgtccgtt
      781 ctcgcgtatg ccggctctcg gatctcggcg gcgtgccgct gcacccgacg ccgctctact
      841 cgatcctctg gaacgtcgtg ccggctctgc tcggcgcgcg gctctgggcg gtgcgcgccc
      901 cgctgcccgt catcgtcggc gtctacctgc tgctcgcggg gatcggccgg ttcgtggagg
      961 aatcccggcg cggcgagccg cagacgcccg tcttcgcgcg cctgcgcctc taccagtgga
     1021 tggcggcggg taccgcggcg gcgggcgccg ccgtcacggg gctgagggcg accgtcgtca
     1081 cgcccgcgcc gcaaccttcc gccgcggcgc tcggaacggc cgtgttgttc gcggccgcga
     1141 cctggttcgc cctcggcgtc gatttcccgc gctcgaaccg ccggttcgcg aggctggtat
     1201 gagaacggac tttctcttgc gcatccacgc actgttgctg gtcgcgttcg tcgcggccgc
     1261 ctcggcggcc gcccaggagc cgccgaagaa ggggaaggcc ttcgagccgc ccccggagcc
     1321 cgagcggcgc tgggagttga cgctgggcgg agcgggcggg ttcgcgtcct cgtcgaggga
     1381 cgtcgacgcc gcgttcgggg cggcgggcta cacgccgtcc tcgtcgggcg actttctctc
     1441 ggcaacggtc ttcccgtcga tccgattccg cctcggcgag cgcgccgccg tcggagtctc
     1501 gttcagctcc acgaagctcg gctcgacgac ggggaccggc ttcggcacga gcgtctccat
     1561 cgggcggtcg tcgacggacg tcgcgctcgt cgccttctgg cgcccgttcc cgggccttcg
     1621 cgtcggcgcc ggtcccgcgt ggtaccggct caccgcggcg ccggacgtcg gggagagcct
     1681 cgaggtctcg aaactcggat ggatcgccga ggcgggtctg gcgtatccgg accgcggcgc
     1741 gtggtacgtg gacctcgccg tccagtatcg cgggaccgga gatgcggact tcggaacgta
     1801 cgtgccgtcc gcgaagggac cgatcgcgcc ggcgccgatc tccctcgacg ggatcggctg
     1861 cgagcacggg gccttcctcg cgggaatcgg cttccggttc tgagaccccg tcagcgcccc
     1921 tcgaccccga cccagccccg cgtgccgtag tcggcgagca ggttgacgaa caggatgagg
     1981 agcatgaaga gcccggagaa gatcacggcg gggatgagcc gcgtgttctc cttcacgtgc
     2041 atgaagtaga ggacgacgag cgtcgccttc gcgatcgcga tcgcgagggc gacggcgttg
     2101 ttggcccagc cgaggtcgac gtaggccgct ccgaccgtcg ccgccgtacc gaccatgagc
     2161 gcggcgaaga ccaggaagta cgtcgacagg ggcatcacgt gcggggcggg agcggacgtg
     2221 gaggcggagg tctcgtgctg cgccatcagt ggtgccgtcc gatgaggtag aggagcggga
     2281 agaggaagat ccagacgaga tccacgaagt gccagtagag gccgaagccc tcgaggaagt
     2341 tgtggttccg tggcgtccag gcgccgcgca gcgccgggcg cagcatgaag acgaggattc
     2401 ccgcgccgat gaccatgtgg agggcgtgga ggccggtcat cccgaagtag aggctgaaga
     2461 agagctgcgc gtgaggcgcg tcggggccct cgaagtggaa cgagggcccg ggcacgtggt
     2521 ggtgcgtgaa cttgtcggcg tactcgaccg ccttcacggc gaggaacgtc gagccgagga
     2581 ggagcgtccc gaggagcatc gcgaacgtcg tcttcggctt gccggtctgt ccgccgcgca
     2641 cggcgagcgc catcgtgagc gagctcccga tcaggacggc ggtgttgaac gcgccgagcc
     2701 ggatgtcgag cgtgtggctc gcggcgcgga acgcctccgg gtagagcgag cggtagaggc
     2761 tgtacgccgc gaagaggccg ccgaagaaca ggatctccgt gacgaggaac gtccacatcc
     2821 cgagcgtcgc ggattcccgc tgctgctcgt agctgtcgaa gtggtgggcg aggtccgagg
     2881 ggccgtggct ctcaaccgac acgggcggcc ccctttccgg ccgcgggctg cgcctcgtac
     2941 gtgtagggcg gagccgtgac cacgggcgga tacgtgaagt tctcgaccgg cggcggagag
     3001 gtcgtctgcc actcgaggcc ggtcgcgccc cacgggttcg cggtcgcgcg cgggccgtac
     3061 cgcatggacc agacgaagta gacgagcggc atgaggtagc cgatgccgag aatcgtcgcg
     3121 ccggccgacg acatgacgtt caggacctgg aactccggcg ggtacgtgtg gtaccggcga
     3181 ggcattccga ggtagccgag gatgaactgc gggaagaacg tgacgttgaa cccgatgaag
     3241 atcacgagag cggacagctt cgaccagaag acggggtaga gccgccccgt gatcttcggc
     3301 caccagaagt gcaggccgcc gaggtagccc atgatcgcgc cgccgaccat gatgtagtgg
     3361 aagtgggcga tgatgaagta cgtgtcgtgc acgtgcgcgt cgatcccgag cgtcgcgagc
     3421 atgacgcccg tgaggccgcc gatcgtgaag aggccgatga agccgagcgc gtagagcatc
     3481 ggcgtgtccc acgagatcga gcccttgtag agcgtcgccg tccagttgaa gaccttcacg
     3541 gccgacggga ccgcgacgag catcgagagg accgagaaga cgacgcccgc gtagatggac
     3601 tggccggcca cgaacatgtg gtggccccac acgaggaagc cgaggatcgc gatcccgatg
     3661 gaggcgaacg cgacgaactt gtagccgaag atcggctttc gggagaacgc ggccacgagc
     3721 tccgagatca cgcccatggc cggcaggatc atgatgtaga cggcggggtg cgagtagaac
     3781 cagaagaggt gctggaagag caccgggtcg ccgccgagct tcgggtcgaa gaacccgatg
     3841 tggaggacgc gctcggccgc gaccatgagg atcgcgatcg cgacgacggg cgtcccgagg
     3901 atctggatga tgctcgtcgc gtagtggccc cacacgaaga gggggagccg gaaccaggtc
     3961 aggcccggcg cgcgcatccg gtggatcgtc acgacgaaat tgaggccggt gaggatcgac
     4021 gagaagccgg cgatgaagat gccgaggccg accagcagaa ccgaggtgtt gacgtagttc
     4081 gtcgagagcg gcgtgtagaa cgtccagccc gtgtcgacgc cgccgtggag gagggcccac
     4141 agcgtgaagg ccccgccgat cgtgtagagg taccacgaga gcaggttgat gcgcgggaac
     4201 gcgaggtcct tcgcgccgat catcagcggg atcaggaagt tcccgaggac cgccgggatg
     4261 cccgggatca ggaagaagaa gatcatcaac acgccgtgca tcgtgaacag gcggttgtag
     4321 acgtcgggct ggaagaagtc gccctcgggc gtcgcgagct ccatgcggat gaggaccgcc
     4381 atgaggccgc cgagcgcgaa aaagacgctc acggagacga ggtagaggag ggcgatgcgc
     4441 ttgtggtcgc gtgtccagag ccacgacttg aggccgtagg ccgcgttcag gtagtgcgtg
     4501 cgcgggaact cggcgggcac cggattcggc gacgacgtca tttccgggcc tccgcggcgc
     4561 cttcggccgc cttgagcgtc ttcacgtagt tcacgagctg gatcagctcc tcctcggtga
     4621 cctggccctt gaaggtcggc atcacgggct ggtagccctg cacgatcttc gcgcccgggt
     4681 tcaggatgga ctcccggacg tagttctcgt ccgcggtgac cttcgcgccg tcctggagcg
     4741 tgacctgcgt gccgaagagg ccgttcagga ccggcgcgcg ggccgagctg tccgggcggt
     4801 ggcacgtgtt gcacgccttc gcgacgaaga gctgctcgcc cgacgcggcg gcggccggcg
     4861 cgccggcggc cggctcgccc gcgagccagg cctcgtacgc gcgcggctcc atcacgacga
     4921 tcttgccgac catcttcgag tgctcgacgc cgcagtactg cccgcagaag aggtggaact
     4981 cgcccgtctt cgtcgccttg aaccagtagg tcgtgtagcg cgacgggagc acgtccacgt
     5041 ggatgcggaa cgcgggcacg aagaagtcgt ggatgacgtc ctcggagatc atgcggagct
     5101 ggacggcctg tccgaccggg acgtgcagct cgttgatctc gcggcggccg ctgggatgct
     5161 ggatcttcca catccactgg cggcccgtga cccagtactg ggccgcgccg gccggcggac
     5221 ggttctggtc gaagaagacc ttcgtgcccc agccgaagag gacgaggagg aggccgagcg
     5281 ggatgaccga ccagacgagc tcgagaacgt gggacttcgg gttcgtctcg tagacgtcct
     5341 cgccgatctc gccctcgtgc ttgcggcggt acttgaccat gaacacgagg acgaggaccg
     5401 cgatgagcgt cgagaagacg acgagcccgc cgagggcgaa gagcatgagg tggtcgacgg
     5461 agcgcgcgat gtcggagccc tgcacgggga agagcgggga gtccgggagg ttcggcatca
     5521 ggcggccccc gtccggcgct tctcgcgctt gagcagggtg aggacgagcc cggcgaccgc
     5581 gacgatcgtg aggacggcgc cgccgcgcag gaagttgcgc accgtggcgg tgtagcggcc
     5641 caccttcggg tcgtagtggt agcagaggag gaggagctgg tcgacgacgc tgccgagctt
     5701 ctcctcggac gcctcgatga ggccgaggcg gaggtccttg gccggatact cgatgccgaa
     5761 gaagtagcgc gcgatccggc cctgcggcgt caggacgaga acgccggtcg cgtgcgcgta
     5821 ctgcttctgc tcggcgtccc acacgtagcg gaagccgacg gctttcgtga gggcgtcgac
     5881 ggacgccttg tcgcccgtga ggaagtgcca gccctcggcc ccgcccttgc ggccgtactg
     5941 acgcatgacg gggcccttct tcgccgacgc catctcggac gtctcgcgcg ggtcgaagct
     6001 gacggagagg acgtcgaact ctgtcccgac gctcatgcgc agggcgcgca ggctccgcac
     6061 gaggccgtcc gtcgtcatcc cgcagagcat cgggcagtcg aagtacacga gggagagaac
     6121 gacgggtttc ttcccgagat agtcgcgcag gtggacggtc ttgcccgcct cgtcgcgaaa
     6181 cgcgaggtcg agcggaacct ggtcgccgat cttctggtcg aacccgatgt ccttgagggg
     6241 cggggcgggc agcggcgcct cggcgggcgg cgcgccctgc gcggtcgcgc ggagctctcc
     6301 ggagagcgcg agagcgaggg cgagcgcgag cggtgcggaa cgggtcactt cttcacctcg
     6361 ggagcggcag gcggcgcggt gcggggcggg aagcccttcg cggccacgat ctcgagcgcg
     6421 cggtcggcgg ggatgcgggc ccgggtcttc gatgcgtccg tccaggacca tccggcgagc
     6481 gcggcgtcct cccgggcgcg gaggaccttc aggtccttct cggggtcgtc ctgcaggagc
     6541 actccggccg gcaggctctt gacgcgcgcc tcggcgagga cgggcggcgg cgggtcctgg
     6601 gcgacgaggc gcttcttctg ccagacggcc acgccccaca tgagcgcaaa cgcggcgatg
     6661 cacacggccg cgaggacggc gacggagacg gcgacggcct tgaggttcag ctcggagtcg
     6721 acgccccggt tctcagccat gcgcgtggac ctccatcgca gccggcaggg ccgggtcgtt
     6781 gacggggagg agcgggcgtt tcgcgagctc gcgcgcgaac gcggcgagcc agagaccgaa
     6841 gagggcggcc ggcagcgccg cgtccagcca gtggatcgag aggccctcgg ggtgccaggc
     6901 gggcgcgacg agccagaaca ggtcgacgcc gcgcatgagg aggaggagcg cggcgatcgc
     6961 ggagagccgg ccgagatgtt tcttgaagtt cgccgagagg aggaacacga acgggacgac
     7021 gaactggaag agcgcgagcg cgagcgagac ggccatccag ccgcccgtca cgcgcgcctt
     7081 gtagaacgtg atctcctcgg ggaggtcccc cgaccagatg atgatgagct gggagacgga
     7141 gaagtacgcc cagagcatcg tgaaggcgaa gaggagcttg ccccagtcgt ggacgtggcg
     7201 ctgctggacc gcggcgcgga acggcgcgcg cttcgcgagg aagagcgcga cgaggagcgt
     7261 gaaggccatc ccggagatcg cctgcccgcc cacgacgtag atcccgtaga tcgccgagaa
     7321 ccagtgcgcg tcggtcgaca tgaggagatc gatcgacgcg accgagatca ggaggcagta
     7381 gatcgcgagc gccggcgccg cgacgagctg catccgccgc tcgagtccgg cggcgggcgc
     7441 ctcgtcctgt ctcttcgaga ggcggctcag ggcgagggcg aaagccgccc agacggcgaa
     7501 gcagagcgcg aggcgcaggg ccatgcccgc ggggttcatg tacgcggcgc ggtggtggag
     7561 gatctcgtcc gtcgcgacgc acgccgggtc ggcccacggg tagacgtgcc ggaggccgaa
     7621 gaagagcggg acggcgaaga gggcgacgag cggcagcgtg cgcgacgagg cttcgaggat
     7681 gcggcggatg acgaggcccc aggcgccgcc cgtcatgtgc tggagcgcct ggatcgcgag
     7741 cgagccgagc gcgacgccga ggacgagtcc gaaggcgacg agatacgagc gatagaactg
     7801 caccgggttc gcgacgaagc cggcggcgga ggccgcggcg aagacggcgc cgacgacgag
     7861 cgcgcggcgg ccgacgcgcg cgatgtcggc ggcgggcggc gcccaatcag cggccgcgag
     7921 cttgagcggg tgatgttcgg tcatcgggct tcgctgcctt cggaagaaga tttctttgaa
     7981 ggtgaatagg gcgcggaggt cgccggagcc gcgccggggg ccgggtgtgc agcggccgcc
     8041 ccgctttcgc cttctaagaa actcttctgc gccggtgacg cggtccctcc gccggccgca
     8101 gcagcttcga gctttccctc gtcgtctttg gctagaaatt ctttcggcat gttctgtgag
     8161 gcctggaggg tcttcatcca cgcaacgatc gcccagcggt cctccggcgt gatctgggac
     8221 gcgtagctcg acatctggcc gaagccgttc gtcatgacgt cgaagaagta gccggcgcgc
     8281 tcgttgcgga ggcggtcgac gtggaaggtg ctcgggctct tgaatccgcg ctggacgatc
     8341 atgccctgcc cgtcgcccgt ccggccgtga cacggggagc agaagatgtc gaagcgctcc
     8401 cgcccgcgga ggaggagcgg cctcgtgacg gggacgggga tgtcgaggac gaacttgccg
     8461 tcctcggcca cgccgcggta gatcgcggcg tccgcgcgca ggaaaccgcg tgcgacgacg
     8521 ccctcgggaa gcggccgggc cgagctgtgg tccgcgaaga acggcgactc gcgcagcggc
     8581 ttcgtcttgg cctggttgtg catgtcgttg cggcagccgg cgagcgcgag agcgagaagg
     8641 gggagaagaa gcgtgcgccg catcagtcct ccacgtcctg gacgtggacc gcgccgagcg
     8701 tctccatgaa ccggaacgtg tcgccggccc ggaatttcgg gtctcgggcg aggatcatca
     8761 ggaagtattt gtcgcgcgac gcgagctgga agcggtccac gccgaagacc gggtggtagg
     8821 gcatcgggag gccgttcagc acgagcatcc cgagaacggc cgcgatgccg gccccgagaa
     8881 tcgtgcactc gaacgtgatc gggatgaacg cgacccagct gttgagaggc cgaccgccga
     8941 cgttgagcgg gtagtccacg acggccgtcc agtactgaag gaagaagccg ccgaggaaac
     9001 cgagcatccc gccgatcagg acgaggagcg gcacgcgcga agggccgtgg ccgatcgcgt
     9061 cgttgagctc ctcgatcgga tacggcgtgt acgcctccat gacgacgtag ccctccgcgc
     9121 gggcgcggtg cacggcctgg acgagggcgg gcgagcccgc gaactcggcg atgacgccgt
     9181 agaggtgggg cggcagctcc ccgcctccct cgcgcggacc gggcttgccg accccggggg
     9241 cgacgtggcg ggggaagagg cgcgacagga ggctcacgcc ttcacctccg gcgccttgtc
     9301 cgccggatct cccgtcgcga tcgtgcgcat ctcgaagatc gagatgacgg gcaggaagcg
     9361 gatgaagagg aacatgagcg agaagaagag gccgagcgac ccgatgtaga aagcccagtc
     9421 ccagcgggtc ggcacgtaca tgtcccacga ggacggaagg aagtcgcggt ggaggctcgt
     9481 cacgacgatg acgaaacgct cgaaccacat gccgatgttg acgacgatcg agatgacgaa
     9541 gagcgtcgcg accgagttgc gcaccttccg gagccacagg agctggggcg tcaggatgtt
     9601 gcagagaatg agcaggaacc agatccagcc gtacggtccc aggatccggt tcttgatcat
     9661 gaaggcctcg aaggggttgc cgctgtacca ggccatgaag atctcgatcg cgtagccgta
     9721 tgccacgatg aggcccgtcg cgagcatgat cttcgccatg ttctgcaggt gccgcatcgt
     9781 gatgaagtcc tcgagcccga acagcttgcg gagcgggatc gagagcgtca tgaccatcgc
     9841 gaagccggag tagatggcgc ccgcgacgaa gtagggcggg aagatcgtcg cgtgccagcc
     9901 cgggatgatc ccgaccgaga agtcgaagct tacgaccgtg tggacggaga cgacgagcgg
     9961 cgtcgcgagg cccgcgagga ggagcgacgc gacctcgtag ttgtgccagt gccgcgccga
    10021 gccgcgccag cccatcgcga gcatcccgta cgcgaccttt ccgaaccagg actttgagcg
    10081 atcgcggagc gtcgcgagat ccgggatgag gcccacgaac cagaagagcg ccgagatggt
    10141 cgcatacgtc gaaacggcga agacgtccca catgagcggg ctgcggaact gcggccacag
    10201 gcccatcgtg ttcgggtacg ggaagagcca gtaggccgcg agccactgcc ggcccgtgtg
    10261 gaagaggacg aactggccgg cggccgcgac ggcgaagaga gtcatcgcct cggcgaaacg
    10321 gttgatcgac gtgcgccacg actggcgcag gaggaggagg atcgcggaga tcagcgtccc
    10381 cgcgtggccg atgccgatcc accagacgaa gttcacgatc gcgaatcccc agccgaccgg
    10441 gatgttgatg ccccagaccc cgacgccgag gagcaggagc ttcgccatcg acatcaggag
    10501 gagctgcagg gccacgaacg agacggcgaa gcccaggaac caccacttcg gcgtcttgtt
    10561 gaggacgacg gcgctgatct tgtccgtgac ggtggccggc gtgtagcccg gcgcgatgac
    10621 gggggggcgt tccggaatcg cgctcatccg tggtgctccg tcgcagccgg cgcgccgccc
    10681 agcttcgggt ttggattcgt gagcttcgcg agatacgtcg tccgcgggcg cgtgttgagc
    10741 tcggcgagga gcgggtactc gagcgggtcc ttgcgcagct tcgcggcctt cgacgtcgga
    10801 tcgttgaggt cgccgaagac gagcgcctcc gtcgggcagg cctgctggca tgccgtcacg
    10861 acctcgccgt cgcggacgcg ccggttctcc ttctcggccg cgatccgggc gcgattcacg
    10921 cgctggatgc agtagttgca cttctccatg acgccgcgct ggcggaccgt gacgttcgga
    10981 ttcgcgagaa gcgtgagaac gggaaggtgc ttgtccttgt tgtagtcgta gaagttgaac
    11041 cgccggacct tgtaggggca gttgttcgaa cagtaccggg tcccgatgca gcggttgtag
    11101 gtcatctcgt tgatgccttc ctcgctgtgg gtcgtcgccg cgacggggca gacgacttcg
    11161 cacggggcct tctcgcagtg catgcacatg accggctggt tgtgggcggc tgggtcctcc
    11221 ggcgtcccct cgtagtagcg gtcgatccgc agccagtgca tctcgcggcc gcgacggacc
    11281 tggtccttgc cgacggtcgg aatgttgttc tccgcctggc aggcgacgac gcatgcgctg
    11341 cagcccgtgc acgaggacag gttcaccgcg aggccccagg cgtgctgctc gtatttccag
    11401 tccgggtaga gcgagatctc cgggacctcc tccatcttct tcgcgaactc cgggtccttg
    11461 cggtacgtct cgtaggtgcc ggtccgtacg aggtgccggc cctccatgtt gaagtgctgc
    11521 tgggtcgagg cgagctctac cgcgcggccg gtcttgcgca gcgccgccgt cgcgttccag
    11581 agcgacgccg acgtgcggag cgcgtatgcg ttgacgccga cgccgttccc gacgcggccc
    11641 gcgcgcgtgc gcccgaagcc gaggtggacc gtcacgacgc cgtcgggctg gcccgggagg
    11701 atccagaccg ggagctcgat cttccgctcg ttcgcctcga tctcgacgac gtcctcgttc
    11761 ttcaggccga gcccttccgc gctcttcggc gagaggtgcg cgacgttgtc ccacacgagc
    11821 ttcgtgaggg gcttcggcat ctcctgcagc cagccgctgt tcgcgaaccg gccgtcgaag
    11881 aggcccgggt ccggcctgag aacgagctcg aacgaccccg ccggcacagg ggtggcccgt
    11941 ggagaggcgg ccggcgcctt cggcgcgagg gccagcggcg cgagggccgt gccttcgacg
    12001 aggccgtcgt gaagggcctt cctccagaac gcgtcgaatc ccgcgggacg ctgtttctgc
    12061 cacgccgcct tcacgaggtc gccgcccttc gcggacggct ggccgaggac ggccgcgagg
    12121 acctcgtggg cggacttgcc gccgtagagg ggctcgacga ggggctgcgc gatggagatc
    12181 gtgccgtcga aggcgcgcgc gtccgtccac gcctcgagcg ggtgggcctc gttcagctgc
    12241 cacgtgcagt acgcgctcgt ctcgtcgtcg tagagcgaca ggtgcacgcg gaacgcggcc
    12301 ttctggacgg cgtccgcgaa cccgaggtcc gcgggggcgt cgaagacggg gtttccgccg
    12361 aggatcagga gcgtgtcgac cttgccggcc ttgaggtcgg cggcgagatc gcggatcgac
    12421 gcgagctggt cgacgggctc ggcctcgacc ggtcccgtga ggaggacggt cgtgccgatc
    12481 gcgccgaggg cgccgttgat cgcgtggacg agggcgtgga cctcggccgg ggcgtagtcg
    12541 cccgcgacga cgaggccgcg agcgccggcg cccttgaggt cggccgcggc cttgatcggg
    12601 atcttcttgg agccgtatag cacgatcgtc gcagccgaat acggacgcga gaactgcacc
    12661 tgcttggcgc gctcgggcgt caccgcaagc gatgcgacca gcacgtcgac cttgttcgtc
    12721 agcaggaacg ggatgc
//
LOCUS       DAOMFY010000095        12724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1526_length_12724_cov_6.986424, whole genome shotgun sequence.
ACCESSION   DAOMFY010000095 DAOMFY010000000
VERSION     DAOMFY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_12724_cov_6.986424"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="PLB01_09495"
     CDS             <1..1833
                     /locus_tag="PLB01_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77575.1"
                     /translation="LALSAAPAVASVVAGSSLVVTVALGQPAVAADAVATLTLAGLPA
                     GLTGSFSPSKMDEPGTGIVSRGQNALLTLAAAASTAAGTYTLTLAAASGAVVRRVSLF
                     VTVGAQPAAPPPGPGVQAPVVLDVFGAGGSHYTSDLVAVNRGSSDATLLLRFAQTPAP
                     ATPGPTIARSLGAGRQFAVGDVIAFLNANGYSLPTDGSAKLGTLFATFVGASDPLSVF
                     VGSRTSTPNPNATVGGAFGTFAAAFATGGASNTDAWIYGLRENASYRSNLALVHAPGA
                     ASGATSGPVSLEVQIYDGDSGAPVGAPLARTLQAGEFFQYNRVLTLAPTGVANGYARI
                     RLTSGTDRYIAYGVVNDGAAAGGGTSDGSFLPANATEGLIPIVLDLPGATHYTSDLTL
                     TNPTSSAVTVTLTYTASTAFSGAGSGTKTATLGARQQLVVPNAIDYLRGLGLAIPATG
                     GQGGTLLVSGAPALARTSNPNPDTGVGGTFGLAYPAVGASARARSEAWVYGLRQDADA
                     RTNLAIADARAGNVAGVEYVVDVFDADTGATTPVLTKTVALAGGQWTQFNTILAEAGI
                     AHGYARVKPASGPSDFVVYGVVNDGPTAGSRTSDGSYVPMVVVN"
     gene            1863..2260
                     /gene="rnpB"
                     /locus_tag="PLB01_09500"
     ncRNA           1863..2260
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLB01_09500"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2259..4313)
                     /locus_tag="PLB01_09505"
     CDS             complement(2259..4313)
                     /locus_tag="PLB01_09505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ77576.1"
                     /translation="MQALRRTSVVAALLACAAGILLLRQVLLRAPIILVDLEDRFAAS
                     LGPLAARLQFPNVVDGLLVAFLGAGFLALALAWKRNGLPSPARDVRLDGPAHPFLSRG
                     VPSLACLTVLAAAELWTGAVMFRDEIPPAWLWLTGLAGFVALAVLADRKAAAAPDSSP
                     PVRAAEVAGVALVAAGSAAVAGYRLHGWEWAGSPDSYGFYGVARDLFEGRLQRPFLSP
                     DGVFGMYAIASSWLQAILFPFSGPTGAALRAGNLVLVAALAALGHVFARPLLGRTGAF
                     ATSVLLGSSVLVQTYAKNGYNNLQGLFFAMLTLTLASRAAATRRWTPLVLSGLAAGAG
                     LLSYALAAISGAAVLLWIALSARPSKRSFRAVVVFGLASLAAAAPCILSRYYVEVHLD
                     KVSPFSPELKEAGPTRPPLEGRVAQALVQPFYNLEPGHAAAGPRVDPVSALLLLAGAA
                     ALVAGVGARAASRALLAVCAFWPFAIGLLQPYFSPTSPWVLLATPAWAILGGSGVAAL
                     ASAIPLARVAGAVALAALPAAAGWSTWQVHESSFASAPIPPLSFTVGVAQAAGASGVP
                     TAFLVPMLSAGEGSPVLEAVKAFAPSVKAESTTFDDPSLPARLEALRDEPAILVLYAG
                     GEIPARDGLYAIARAAWPGAREVRYRSLPSAAGEPSVQVFVNARARAAVPFLPARNRL
                     EE"
     gene            4362..4817
                     /locus_tag="PLB01_09510"
     CDS             4362..4817
                     /locus_tag="PLB01_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQQ77577.1"
                     /translation="MPEIVDGNNLIGRLGGGSREGLVTELCEVARRKRKRLTVVFDGP
                     PEAGRAKVQAFGDVQVVYAAPRSADEEIIRRIREARDPRGVTVVTDDRALASAVAAAG
                     GRTAGIEFFQRDASARMVASPAAETGKPAAAGSPKEWERWFSDPKNRLA"
     gene            4817..5773
                     /locus_tag="PLB01_09515"
     CDS             4817..5773
                     /locus_tag="PLB01_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQQ77578.1"
                     /translation="MGNLVRLPRIAPGDTIGVAALSGPADTEKLAAGVRALEEFGYRV
                     RLAPNVLAGEPLLGLAGNAEERAAGYRALLVDPGVKAIVFARGGYGVAAALPYLDASE
                     AAFHPKIHCGFSDATVLSSWLLKAGVPSFHGPMVAADLARGLDPLSASFFPSMLEGRG
                     PGRIVFEGEEGKGGGIGSSVLVPGAAKGRLVGGCLSLLAASVGTPWEYDYSGGLLFFE
                     EVSEEAYRVDRMLGTLIDSGRFAKLSGILIGSMSAVTFGGAEDASRLRAVLEARLAPL
                     GIPVAIGLPAGHRAPNVTLPIGARASWDGAGTLTFLEEIVSP"
     gene            5770..6552
                     /locus_tag="PLB01_09520"
     CDS             5770..6552
                     /locus_tag="PLB01_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQQ77579.1"
                     /translation="MKVVVKSGTPSGFADYLMKATAGKDLFVEGEVGTEMYVIQAGTV
                     EIYKTSRKGEEKILATLEKGDFFGEMSILEDVPRTASARAKTDCELVRINQTTFDEML
                     RHNAEIAVRMLRKLSRRLRETTKLLEQSTGVAPTLDQSSSDIFAKEADRHDIFRLVAD
                     AGGEFFLNREGETTVGRIDPVTGIRPDVDLANLDTQRSVSRRHSKIVHSGNEFRVVEE
                     IGTMNGTFVNGTRIVTGQPVPIKDGDRIRFGLVDLTFRISKA"
     gene            6588..8678
                     /gene="recG"
                     /locus_tag="PLB01_09525"
     CDS             6588..8678
                     /gene="recG"
                     /locus_tag="PLB01_09525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQQ77580.1"
                     /translation="MPGIGPARATELADAGVETALDLLLHLPFRYEDRTRIGPIADLV
                     AEGPATTVKGTVLSSRLIRTRRRGFTIFEAILDDGSGTLRLVFFNQPYLQRWMAPKKV
                     FWVYGTPTLLSRFRRGLGMENPQIEDFLEGKESDGEGDSDPLSVGRVVPIYRKLPGLT
                     PRLRRRVVFHLLRNFADHLPERLRNSREREQDLPPLGESLREAHFPGSAGGPADAEAW
                     TERKSPHLHRLVYEEFLEFQVGLQRRRAERDRVPAPVLAADDAIRARLKEALPFSLTA
                     AQKRVIREIGEDFRAGRPMRRLLQGDVGSGKTIVAVLSAILAAECGRQTALMAPTEIL
                     AEQHFTTIFRIFGKTRFRAELLTGRVKGKPRAQVLDALAGGRIDMLVGTHALLEEPVE
                     FRRLGLIVIDEQHRFGVAQRTALAAKADPSGARPHLLVMSATPIPRSLALTLYGDLDV
                     STLDEKPPGRTAITTRVLPESERAAAFREIEGEIRAGGQAYVVVPLIEESDTIDARAV
                     EKHAEEIRRALPGRRVGVLHGRIPPDERERTMGEFAAGKLDVLAATTVIEVGVDVPNA
                     SYMLVENAERFGLAQLHQLRGRVGRGRRKSRCVLLFGAKTSPESRERLVVLEKTDDGF
                     VVAEEDLKRRGPGDALGTRQSGVPMFRVGDLVRDVRWMKDAKEDATRLIAEGRDAAFR
                     EPLFLRPGTGAGGD"
     gene            8745..9383
                     /locus_tag="PLB01_09530"
     CDS             8745..9383
                     /locus_tag="PLB01_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQQ77581.1"
                     /translation="MDPQKTLEIVIQVVVLLFAVSFHESAHGFVAMKCGDNTARDLGR
                     ITMNPLKHLDPIGSLFFPAVLAFSGLPVFGWAKPVPVSLRGVRNPRRANLLVSAAGPV
                     SNLILAIVFAVLTFALRPVVTTRQHVLFPLLLVAVLSVLVNVSLAIFNLLPIPPLDGF
                     GVLESLLPASALPAVMFLRRYGFIILMLLMFTGVLSFILRPAREILVGWLLR"
     gene            9380..10378
                     /gene="trpS"
                     /locus_tag="PLB01_09535"
     CDS             9380..10378
                     /gene="trpS"
                     /locus_tag="PLB01_09535"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQQ77582.1"
                     /translation="MSANPKPQIVLSGLRPTGRVHLGNYWGAVKNWVDLQEKYACRYF
                     VADLHALTTDYADTSAIREASVEAVTDWLSVGLDPQKSVIFVQSRVPQTAELALVFGM
                     ITPLGWLERVPTYKEQIEQLRERDLGTFGFLGYPVLMTADIALYLAHYVPVGKDQESH
                     LEICREIVRRFNGFYGDVFPEPKALFTATPKVPGLDGRKMSKSYGNTLALSDSAEDVT
                     KKVMGMVTDPGRARRSDPGDPDVCNLYPFHVLYSPPEEQKIVADECRSAARGCVDCKK
                     HLIRNMNAALEPIRQKRAEIVAQKDYVRDVLETGNGKARQIASETMEKVSDAMGLL"
     gene            10430..11239
                     /locus_tag="PLB01_09540"
     CDS             10430..11239
                     /locus_tag="PLB01_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQQ77583.1"
                     /translation="MAEFRIPVPAGADLMQPYPVKVPQFEGPLDLLLHLIRKNEVDLR
                     NIPVADICRQYHEYLVLMQELDLEVAAEFLYVEALLVHVKSQMVLPRAVLPDGTPQED
                     PRDELVRRLIEYRKYKGVAETLHEMEALRLGLWSRPPAKLEATETEAEETDLSEVSLF
                     DLLTFFKGALDRYRSAHPPSMEIAHQKYSIKEKMESMLARVRSGTGPVPLSDVFKALS
                     GRAEAIAVFLAVLELLRLRVVRARQEAEFSDILLESTGEDVTLEGYEESYR"
     gene            11236..11895
                     /gene="scpB"
                     /locus_tag="PLB01_09545"
     CDS             11236..11895
                     /gene="scpB"
                     /locus_tag="PLB01_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQQ77584.1"
                     /translation="MTDDVETPETPPAAAPSAPPAHVALVEALLFASGKPATVERLVE
                     ASELKAAEVEAALEVLERALEADARGIRLDRVAGGARLVSRNEFDVPVRKLLGLEGKN
                     KLSMASLETLSIIAYRQPVTAPEIAELRGVNSASSLRTLLDKKLITTAGRKEVVGTPF
                     LYKTTKEFLVHFGLDDVKDLPKPEDLDALYGVETNTSQTELFPEPEQEREEKEEDPSN
                     G"
     gene            12003..>12724
                     /locus_tag="PLB01_09550"
     CDS             12003..>12724
                     /locus_tag="PLB01_09550"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQQ77585.1"
                     /translation="MPAERLQKLLSAAGISSRREAEKIILEGRVTVNGHKVTELGVKA
                     DPAKDHVKVDGKLLRIAVVRRYVLMNKPKGLIVTRDDPGGIPTVFELLKGRVGERVVA
                     VGRLDVESEGLLLLTDDGPLVHKLTHPSGGCRKEYEVKVSGVPSPAQLERLRRGVTLP
                     DGVRTAPAEITLSETTPEKNGVGGNAWLKVILGEGRSRQIRRMFDLVGHPVTKLRRVA
                     IGPIRDSKLPPGAFRDLSPEEVA"
BASE COUNT         1991 a         4524 c         4420 g         1789 t
ORIGIN      
        1 ctcgccctgt cggcggcgcc tgcggtggcg tccgtcgtgg cgggctcgtc gctcgtcgtg
       61 acggtggccc tcgggcagcc cgcggtcgcc gccgacgcgg tcgcgacgct cacgctcgcg
      121 ggcctgcccg cggggctgac gggttcgttc agcccctcga agatggacga gcccgggacc
      181 ggcatcgtct cccgcggcca gaacgcgctt ctgacgctcg ccgccgcggc ctcgacggct
      241 gccgggacgt acacgctcac gctcgcggcc gcgtcgggcg ccgtcgtgcg gcgcgtgtcg
      301 ctcttcgtca ccgtgggggc gcagcccgcc gcgcctccgc ccgggccggg agttcaggcg
      361 ccggtcgtcc tcgacgtctt cggcgccggg ggctcgcact acacgtcgga cctcgtcgcc
      421 gtgaaccgcg gctcctcgga cgccacgctc ctcctgcggt tcgcacagac gcctgcgccc
      481 gcgacgccgg ggccgacgat cgcgcgctcg ctcggggccg gccgccagtt cgccgtcggg
      541 gacgtgatcg cgttcctgaa cgcgaacggc tactcgctcc cgacggacgg gtccgcgaag
      601 ctcgggacgc tgttcgcgac gttcgtggga gcgtcggatc ccctgagcgt cttcgtgggc
      661 tcgcggacgt cgacgccgaa cccgaacgcg accgtcggcg gcgccttcgg gacgttcgcg
      721 gccgcgttcg cgaccggcgg cgcttcgaac acggacgcgt ggatctacgg actcagggag
      781 aacgcgtcct accgctcgaa cctcgcgctc gtccacgcgc ccggcgcggc ctcgggggcg
      841 acgtccgggc cggtctccct cgaggtccag atctacgacg gcgacagcgg cgcgccggtc
      901 ggcgccccgc tcgcccgcac gctccaggcc ggggagttct tccagtacaa ccgtgtcctc
      961 acgctggcgc cgaccggggt cgcgaacggt tacgcgcgca tccgcctgac gtccgggacg
     1021 gaccgctaca tcgcgtacgg cgtcgtgaac gacggcgcgg cggcgggcgg gggcacgtcg
     1081 gacggcagct tcctcccggc gaacgcgacc gaggggctga tcccgatcgt tctcgacctg
     1141 ccgggcgcga cccactacac gagcgacctg accctgacga acccgacctc gagcgcggtc
     1201 acggtgaccc tgacctacac ggcgtcgacg gcgttttcgg gcgcgggctc cggcacgaag
     1261 accgccacgc tcggcgcgcg ccagcagctc gtcgtcccga acgcgatcga ctacctgcgc
     1321 ggtctcgggc tcgcgatccc ggccacgggc ggccagggcg gcacgctgct cgtctcgggg
     1381 gccccggcgc tcgcgcgcac ctccaacccg aatccggaca cgggtgtcgg cgggacgttc
     1441 ggcctcgcgt acccggcggt gggcgcgtct gcgcgcgcga ggagcgaggc atgggtctac
     1501 ggcctcaggc aggacgccga cgcccgcacg aacctcgcga tcgcggacgc gcgggcggga
     1561 aacgtcgccg gcgtggaata cgtcgtggac gtcttcgacg cggacacggg cgcgacgacg
     1621 ccggtcctga cgaagacggt cgcgctggcc ggcgggcagt ggacgcagtt caacacgatc
     1681 ctcgccgagg cgggcatcgc ccacgggtat gcgcgcgtga agccggcctc cgggccttcg
     1741 gacttcgtcg tgtacggcgt cgtgaacgac ggcccgacgg ccggctcgcg gacgtcggac
     1801 ggttcgtacg tcccgatggt ggtcgtgaac tgacgggcgg acgcggtaga ctgcccgcga
     1861 ctcgggccgg acaggcggtc gccgctcttg acgcctcggc ggaaggaggg gaggaaagtc
     1921 cggactccag cgggcagcgt gccggagaaa ttccggtcgg ggcaacccga aggaaagtgg
     1981 cacagaaaac ataccgccga cgcgccgcga ggcgcaggta agggtgaaaa ggtgcggtaa
     2041 gagcgcaccg cggtcccggc aacgggactg gcaggcgaaa ccccacgcgg agcaaggcca
     2101 aatagggtga cggaaggggc gcccgccctc gcggctcacg ccgacgtcac cgggtcggtc
     2161 gctcgagccc gtccggaagg acgggcctag aggaatgacc gccagccgga aggcgcgagc
     2221 cgaccggtca cagaatccgg cttatcgtcc ggcccgagtc attcttccaa ccggttgcgc
     2281 gcgggcagga acggcacggc cgcgcgtgcg cgggcgttca cgaagacctg cacggacggc
     2341 tccccggccg ccgacgggag agatcgatag cgcacctcgc gcgcccccgg ccaggccgcc
     2401 cgggcgatcg cgtagagccc gtcacgagcg ggaatctcgc cgcccgcgta gaggaccagg
     2461 atggccggct cgtcccggag ggcctcgagg cgggcgggca gagaagggtc gtcgaaggtg
     2521 gtcgattcgg ctttcaccga aggcgcgaag gccttcacgg cctccaggac cggcgagccc
     2581 tctcccgcgc tgagcatggg gacgagaaac gcggtgggga cgcctgaggc gcctgccgcc
     2641 tgcgccacgc cgaccgtgaa ggagagcggc gggatgggcg cgctcgcgaa cgaggactcg
     2701 tgcacctgcc acgtgctcca gcccgccgca gccggcaacg ccgcgagcgc caccgcgccc
     2761 gccacccgcg cgagcgggat cgccgaggcg agcgcggcga cgccgctgcc gccgaggatc
     2821 gcccaggccg gcgtcgcaag gaggacccag gggctcgtcg gagagaagta gggctgaagg
     2881 aggccgatgg cgaacggcca gaaggcgcag acggcgagca gcgcgcgcga ggctgcgcgg
     2941 gctccgaccc ccgccacgag ggccgcggct ccggcgagca gcagcagggc ggagacggga
     3001 tccacccggg ggccggcggc cgcgtgtccg ggttcgaggt tgtagaaggg ctggacgagc
     3061 gcctgcgcga cgcggccctc gagcggcggc ctcgtggggc cggcctcctt cagctcgggc
     3121 gaaaaggggg acaccttgtc gagatgcacc tcgacgtagt aacggctgag aatgcagggc
     3181 gcggcggccg cgagcgaagc gaggccgaac accacgacgg cgcggaacga tctcttcgac
     3241 gggcgggccg agagcgcgat ccagaggagg accgccgccc cggagatcgc cgcgagcgca
     3301 tagctgagaa gacctgctcc cgcggcgagt cccgacagga cgagcggcgt ccagcgccgc
     3361 gtcgcggctg cgcgcgaggc gagcgtgagg gtcagcatcg cgaagaagag gccctggaga
     3421 ttgttgtagc cgttcttcgc gtacgtctgg acgagcaccg acgacccgag gaggacggac
     3481 gtcgcgaagg cgcccgttcg cccgaggagg ggacgcgcga acacgtggcc gagcgccgcg
     3541 agggccgcga cgaggacgag gtttccggcg cggagcgcgg cgccggtcgg ccccgagaac
     3601 gggaacagaa tggcctggag ccaggacgag gcaatcgcgt acatcccgaa gactccgtcg
     3661 ggcgagagga aaggccgctg gagtctccct tcgaagagat cccgggcaac gccgtagaac
     3721 ccgtaggaat ccggggatcc cgcccactcc cagccgtgga ggcggtaacc ggcgacggcg
     3781 gcgcttcccg cggcgacgag agcgactccc gcaacctcgg cagcgcggac gggcgggctg
     3841 gagtccggcg cggccgccgc cttccggtcc gcgagaacgg cgagcgccac gaagccggcg
     3901 aggcccgtga gccacagcca cgcgggcgga atctcgtcgc gaaacatcac ggcgcccgtc
     3961 cagagctcgg cggcggcgag gaccgtcagg cacgcgagag acggaacgcc gcgggagaga
     4021 aacgggtgcg cgggtccgtc gaggcgcacg tcccgcgcgg gcgacgggag gccgttccgt
     4081 ttccaggcga gggcgagggc gaggaatccg gctccgagga acgcgaccag aagtccgtcc
     4141 acgacgttcg ggaactgcag ccgggccgcg aggggcccga gcgacgccgc gaacctgtcc
     4201 tcgagatcca cgaggatgat cggtgcgcgc agcaggacct gccggaggag gaggatgccc
     4261 gccgcgcacg ccaggagcgc cgccacgaca ctcgtgcgtc ggagggcttg cacgggcgaa
     4321 tggtcgcatg cggcggcgcc ggcgtctgcg agaattgccg ggtgcccgag atcgtcgacg
     4381 gcaacaacct gatcggccgg ctcggcggcg gctcgcgcga agggctcgtc acggagctct
     4441 gcgaggtcgc gcgccgcaag cgaaagcggc tcaccgtcgt cttcgacggg ccgccggagg
     4501 cgggacgcgc caaggtgcag gcgttcgggg acgtccaggt cgtctacgcg gcgccccgct
     4561 cggccgacga ggagatcatc cgccgcatcc gcgaggcgcg cgacccgcgg ggcgtcaccg
     4621 tcgtgacgga cgaccgcgcc cttgcgtcgg cggtcgccgc cgccgggggg cgcacggccg
     4681 gcatcgagtt ctttcagcgg gacgcttccg cccggatggt cgcctctcct gccgccgaga
     4741 ccggcaagcc cgccgcggcg ggcagcccga aggagtggga gcgctggttc tccgacccga
     4801 agaaccggct cgcctgatgg ggaatctcgt gcggctgccg cggatcgctc ccggagacac
     4861 catcggcgtg gcggcgctgt cggggcccgc cgacacggag aagctcgcgg caggcgtccg
     4921 cgcgctcgag gaattcggct accgcgtccg cctggcgccg aacgtcctcg ccggggaacc
     4981 gctcctcggc ctcgcgggga acgccgagga gcgcgccgcc ggctaccggg cgctcctcgt
     5041 cgacccgggc gtcaaggcga tcgtcttcgc ccggggaggc tatggcgtcg cggccgcgct
     5101 cccgtacctc gacgcgtccg aggcggcatt tcacccgaag atccactgtg gcttctcgga
     5161 cgcgaccgtt ctttcttcgt ggctcctgaa ggcgggcgtt ccgtcgttcc acggcccgat
     5221 ggtcgcggcc gacctcgcgc gcggcctcga cccgctctcg gcttcattct ttccatcgat
     5281 gctcgagggg aggggacccg ggagaatcgt ctttgaaggt gaagagggga agggcggcgg
     5341 gatcgggagc agcgtcctcg tgcccggcgc cgcaaagggg aggctcgtcg gagggtgcct
     5401 ctcgctgctt gccgcgtccg tcggcacgcc ctgggaatac gactattccg gcggcctcct
     5461 cttcttcgaa gaggtctccg aagaggccta tcgggtagac cggatgctcg gaaccctgat
     5521 cgattccggc cgattcgcta aactgtccgg catcttgatc ggcagcatgt ccgccgtgac
     5581 gttcggaggg gccgaggacg cgtcgcgtct cagggccgtt ctcgaggccc ggctcgcccc
     5641 cctcggcatc cccgtggcca tcggtctgcc cgcgggccat cgcgccccta acgtcaccct
     5701 gccgatcggc gcccgcgcct cctgggatgg agccgggacg ctgacgttcc tcgaggagat
     5761 cgtgtcccca tgaaagtggt cgtcaagtcg ggcactccga gcggtttcgc ggactacctg
     5821 atgaaggcca cggccggcaa ggacctcttc gtcgaaggcg aggtcggcac ggagatgtac
     5881 gtgatccagg ccggcacggt cgagatttac aagacgagcc gcaaaggcga ggagaagatc
     5941 ctcgcgacgc tcgagaaggg cgacttcttc ggcgagatgt cgatcctgga ggacgtccct
     6001 cggacggcgt ccgcgcgcgc gaagaccgac tgcgagctcg tccggatcaa ccagacgacg
     6061 ttcgacgaga tgctgcgcca caacgcggag atcgcggttc gcatgctccg caagctctcg
     6121 cgccgcctcc gcgagacgac gaagctcctc gagcagtcga cgggcgttgc cccgactctc
     6181 gaccagtcgt cgtcggacat cttcgcgaag gaggccgacc ggcacgacat cttccgcctc
     6241 gtggcggacg ccggcggcga gttcttcctg aaccgcgagg gggagaccac cgtggggcgc
     6301 atcgaccccg tcacgggcat ccggccggac gtcgacctcg cgaacctcga cacgcagcgc
     6361 tcggtctccc gacgtcattc gaagatcgtc cactccggaa acgagttccg tgtcgtcgaa
     6421 gagatcggga ccatgaacgg gacgttcgtg aacggcaccc gcatcgtcac gggccagccg
     6481 gttccgatca aggacgggga ccgcatccgc ttcggtctcg tcgacctcac gttccgcatc
     6541 agcaaggcgt aggggtcatt tccgctccgc ttccgaccct cgcgtccctg cccggcatcg
     6601 gacccgcgcg cgcgacggag ctggccgacg cgggcgtcga aaccgcgctc gaccttctcc
     6661 tccacctgcc gttccggtac gaggatcgca cgcgcatcgg cccgatcgcc gacctcgtcg
     6721 ccgaaggccc cgcgacgacc gtcaagggga cggtcctctc ctcgcgcctg atccgcacgc
     6781 gccgacgggg cttcacgatc ttcgaggcga tcctcgacga cggctccggc acccttcgcc
     6841 tcgttttctt caaccagccc tacctgcagc ggtggatggc gccgaagaag gtcttctggg
     6901 tctacgggac ccccaccctg ctctcgcgtt tccggcgcgg gctcggcatg gagaacccgc
     6961 agattgaaga tttcttagaa ggtaaagaga gcgatgggga gggcgactcc gatccgctgt
     7021 cggtggggcg ggtggttccc atctatcgga agctccccgg cctcactcca cggttgcgga
     7081 ggcgcgtggt cttccacctt ctaagaaact ttgcagacca tctccccgag cgcctccgca
     7141 actcgcgaga gagggagcaa gacctccctc ccctcggcga atccctgcgc gaggcccatt
     7201 tcccgggcag cgccggcggc ccggccgacg cagaggcctg gacggagagg aaatcgccgc
     7261 acctgcaccg tctcgtctac gaggagttcc tcgagttcca ggtcggcctt cagcgccgac
     7321 gggccgagcg tgaccgcgtg cccgcgcccg tcctcgccgc cgacgacgca atccgcgcgc
     7381 gtctcaagga ggcgctcccg ttctcgctca cggccgcgca gaagcgcgtc atccgcgaga
     7441 tcggcgagga cttccgcgcc ggccggccga tgcggcgact cctgcaaggc gacgtcggga
     7501 gcggcaagac gatcgtcgcc gtcctgtccg ccatcctcgc ggccgagtgc ggccggcaga
     7561 cggcgctcat ggcgccgacc gagatcctcg ccgaacagca cttcaccacg atcttccgga
     7621 tcttcggcaa gacgcgcttc cgcgccgagc tcctgaccgg gcgcgtgaag ggaaagccgc
     7681 gcgcgcaggt tctggacgcc ctcgccggcg gacggatcga catgctcgtc gggacgcacg
     7741 cactcctcga ggagcccgtc gagttccgaa ggctcgggct gatcgtcatc gacgagcagc
     7801 accggttcgg cgtcgcccag cgcacggcgc tcgcagcgaa ggcggacccg tccggcgcgc
     7861 ggcctcacct cctcgtgatg tccgcaacgc cgatcccgcg ctccctcgcc ctgacgctgt
     7921 acggcgacct cgacgtctcg acgctggacg aaaagccgcc gggccgaacg gcgatcacga
     7981 cacgcgtcct gccggaatcc gagcgcgccg ccgcgttccg cgagatcgag ggcgagatcc
     8041 gcgccggcgg ccaggcctac gtcgtcgtcc cgctcatcga ggagtccgac acgatcgacg
     8101 cccgggccgt cgagaagcac gccgaggaga tccgccgggc cctgccgggt cgtcgcgtgg
     8161 gcgtcctcca cgggcgcatc ccgccggacg agcgcgagcg gaccatgggg gagttcgccg
     8221 cggggaagct ggatgtcctg gccgcgacga ccgtcatcga ggtcggcgtc gacgtgccga
     8281 acgcctccta catgctcgtc gagaacgcgg agcggttcgg cctcgcccag ctgcaccagc
     8341 tgagggggcg cgtgggccgc ggccggagga aatcgcggtg cgtgctcctc ttcggggcga
     8401 agacctcgcc ggagtcccgc gagcgcctcg tcgtcctcga gaaaacggac gacggattcg
     8461 tcgtggccga ggaggacctg aagcgccgcg gcccgggcga cgccctcgga acgcgccaga
     8521 gcggcgtccc gatgttccgc gtcggcgacc tcgtccggga cgtgcgatgg atgaaggacg
     8581 cgaaggagga cgcgacgcgg ctcatcgccg aaggccgtga cgcggcgttc cgcgagccgc
     8641 tcttcctccg gcccgggacc ggcgcggggg gcgattgagc cgccgcgttc cgggttaaca
     8701 tccgcgcccg cgcgacgccc gaagcgccgc gccgaaaagg cgccgtggac cctcaaaaaa
     8761 cgctcgagat cgtcatccag gtcgtcgtcc tcctgttcgc cgtctcgttc cacgagtccg
     8821 cccacggctt cgtcgcgatg aagtgcggcg acaacacggc ccgcgacctc ggccgcatca
     8881 cgatgaaccc gctcaagcac ctcgacccga tcgggagcct gttcttcccc gcggtcctcg
     8941 cgttctccgg gctgccggtc ttcggctggg cgaagcccgt ccccgtgtcg ctgcgcggcg
     9001 tccgaaaccc gcggcgcgcg aacctgctcg tctcggcggc gggcccggtc tcgaacctga
     9061 tcctcgcgat cgtcttcgcg gtcctgacct ttgcgctccg tccggtcgtc acgacacggc
     9121 agcacgtcct cttccccctg ctcctcgtcg ccgtgctctc cgtcctcgtg aacgtgtcgc
     9181 tggcgatctt caacctcctg ccgatcccgc cgctggacgg cttcggcgtg ctcgagagcc
     9241 tccttccggc ctcggcgttg ccggcggtga tgttcctgcg ccgctacggc ttcatcatcc
     9301 tgatgcttct gatgttcacg ggcgtcctca gcttcatcct ccggccggcg cgcgagatcc
     9361 tggtggggtg gctcctccga tgagcgcgaa cccgaaaccg cagatcgtcc tttcgggcct
     9421 tcgcccgacg ggccgcgtgc acctcggcaa ctactggggc gccgtgaaga actgggtcga
     9481 cctgcaggag aaatacgcct gccggtactt cgtcgccgac ctccacgccc tcacgacgga
     9541 ctacgcggac acgtccgcca tccgcgaggc ctcggtcgag gccgtgacgg actggctttc
     9601 cgtgggcctc gacccgcaga aaagcgtcat cttcgtccag tcgcgcgtcc cgcagacggc
     9661 cgagcttgcg ctcgtcttcg ggatgatcac gccgctcggc tggctcgagc gcgttccgac
     9721 gtacaaggag cagatcgagc agctgcgcga gcgggacctc gggaccttcg ggttcctcgg
     9781 ctatccggtc ctcatgacgg cggacatcgc gctctacctc gcgcactacg tgcccgtcgg
     9841 caaggaccag gagagccacc tcgagatctg ccgcgagatc gtccgccgct tcaacggctt
     9901 ctacggggac gtcttccccg agccgaaggc gctgttcacg gccacgccga aggtgccggg
     9961 cctggacggc cggaagatgt ccaagagcta cggcaacacg ctcgcgctct cggattcggc
    10021 cgaagacgtc acgaagaagg tcatggggat ggtcacggac ccgggccgcg cccgccgctc
    10081 cgacccgggc gacccggacg tgtgcaacct gtacccattc cacgtcctgt attcgccccc
    10141 ggaggaacag aagatcgtcg ccgacgagtg ccgctcggcg gcgcgcggct gcgtggactg
    10201 caagaagcac ctcatccgca acatgaacgc cgccctcgag cccatccgcc agaagcgcgc
    10261 ggagatcgtc gcgcagaagg actacgtccg cgacgtgctg gaaaccggca acggaaaggc
    10321 aagacagatt gcatcggaaa ctatggagaa ggtctccgat gcgatggggc tgctttgagc
    10381 attgcttctc ttaaccttct aagaaatccc ggggctcttc ggacctccca tggctgagtt
    10441 ccggattccc gtccccgccg gcgcggacct catgcagccc tacccggtca aggtgcccca
    10501 gttcgagggg ccgctggacc tgctgctcca cctcatccgc aagaacgagg tggatcttcg
    10561 caacatcccg gtcgcggaca tctgccgcca gtaccacgag tacctcgtcc tcatgcagga
    10621 gctcgacctc gaggtcgccg ccgagtttct ctacgtcgag gcgcttctcg tccacgtcaa
    10681 gtcgcagatg gtccttccgc gcgccgtcct gccggacggc acgccgcagg aagacccgcg
    10741 cgacgagctc gtgcggcgcc tcatcgagta ccgtaagtac aagggcgttg ccgagacgct
    10801 ccacgagatg gaggcgctgc gcctcggtct ctggagccgt ccgcccgcga agctcgaggc
    10861 aaccgagacc gaagcggagg agacggacct ctccgaggtc tcgctgttcg acctcctcac
    10921 gttcttcaag ggcgcgctcg accgttatcg ctcggcgcac ccgccctcca tggagatcgc
    10981 gcaccagaag tactcgatca aggagaagat ggagagcatg ctcgcgcgcg tgcgcagcgg
    11041 caccggcccc gtgcccctgt cggacgtctt caaggccctc tccggccgcg cggaggcgat
    11101 cgccgtcttc ctcgccgtcc tcgagctcct ccgcctgcgc gtcgtccggg cccggcagga
    11161 ggccgagttc agcgacatcc tcctcgagtc gaccggcgag gacgtgaccc tggagggtta
    11221 cgaggagtcc taccgatgac cgacgacgtc gagacgcccg agacgccgcc cgctgcggcg
    11281 ccgtcggccc cgcccgcgca cgtggcgctc gtcgaggcgc tgctcttcgc gtcgggcaag
    11341 cccgcgaccg tcgagcgtct cgtcgaggcc tcggagctga aggccgccga ggtggaggcc
    11401 gcgctcgagg tcctcgagcg ggccctcgag gccgacgcgc gcggcatccg gctggaccgc
    11461 gtcgcgggcg gggcccgcct cgtctcgcgc aacgagttcg acgtccccgt tcgcaagctc
    11521 ctcgggctcg agggcaagaa caagctctcg atggcgagcc tcgagacgct ttcgatcatc
    11581 gcgtaccgcc agcccgtcac ggcgccggag atcgcggaac tgcgcggcgt gaactccgcg
    11641 tcgtcgctcc ggacgctcct cgacaagaag ctcatcacga cggcgggccg caaggaagtc
    11701 gtcggcacgc cgttcctcta caagacgacg aaggagttcc tcgtccactt cggcctcgac
    11761 gacgtcaagg acctcccgaa gcccgaagac ctcgacgcgc tctacggcgt cgagacgaac
    11821 acctcgcaga ccgagctgtt cccggagccg gaacaagaaa gagaagaaaa agaggaagat
    11881 ccttcgaatg gttaatgcag caggacgacg agccggagcc gcgccgcacg gccgaggcgc
    11941 catccccgcc tcattacctt ccaagaaaat cttcttctct tcgttctctt cggacgtgcc
    12001 acatgcccgc tgaacgtctc cagaagctcc tgtccgccgc cggcatttcc tcccgccgcg
    12061 aggcggagaa gatcatcctc gagggccggg tgaccgtgaa cggccacaag gtcacggagc
    12121 tcggcgtgaa ggccgatccc gcgaaggacc acgtgaaagt ggacgggaag ctcctgcgca
    12181 tcgcggtcgt gcggcgctac gtcctcatga acaagccgaa gggactcatc gtgacgcgcg
    12241 acgacccggg cgggatcccg acggtcttcg agctcctgaa gggccgcgtc ggcgagcgcg
    12301 tcgtggccgt cgggcgcctc gacgtcgagt ccgaaggcct cctgctcctc acggacgacg
    12361 ggccgctcgt gcacaagctc acgcacccct ccggcggctg ccgcaaggag tacgaggtca
    12421 aggtttccgg cgtcccctcg cccgcgcagc tcgaacgact gcgccggggc gtgacgcttc
    12481 cggacggcgt gaggacggcc cccgcggaga tcacgctgtc cgagacgacc cccgagaaga
    12541 acggcgtcgg cggcaatgcg tggctcaagg tgatcctcgg cgagggccgt tcgcggcaga
    12601 ttcggcgcat gttcgacctc gtcggtcacc cggtcacgaa gcttcggcgc gtcgccatcg
    12661 gcccgatccg cgactccaag ctcccgccgg gcgccttccg ggatctgtcg ccggaggaag
    12721 tcgc
//
LOCUS       DAOMFY010000096        12702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1530_length_12702_cov_7.355104, whole genome shotgun sequence.
ACCESSION   DAOMFY010000096 DAOMFY010000000
VERSION     DAOMFY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_12702_cov_7.355104"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLB01_09555"
     CDS             complement(<1..187)
                     /locus_tag="PLB01_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77586.1"
                     /translation="MAEEKKKISSKGPESRGAAGADAKAAHAPSKKSLRIRQVKSGIC
                     APVDQKQTLFGLGLRRIR"
     gene            complement(187..702)
                     /gene="rpsE"
                     /locus_tag="PLB01_09560"
     CDS             complement(187..702)
                     /gene="rpsE"
                     /locus_tag="PLB01_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQQ77587.1"
                     /translation="MDHVVHINRVTKVVKGGKNFSFSALVVVGDGHGRVGYGAGKAKE
                     VPMAIKKGIEMAKKKMIKVPLKGKTIPHPIVGHYGAGRVMLKPASEGTGVIAGGAVRA
                     VLESAGVQNILTKSLGTTNPHNVVKATFDALLHLQLEETIAKARGRENGEARPAEPAT
                     AAPATEATEKV"
     gene            complement(825..1193)
                     /gene="rplR"
                     /locus_tag="PLB01_09565"
     CDS             complement(825..1193)
                     /gene="rplR"
                     /locus_tag="PLB01_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQQ77588.1"
                     /translation="MQKAQARVEARTRIHARIRTKVKGTAERPRLAVSKTLRWISVQI
                     IDDAAGKTLAHATSREEGVRGKAKSAANKNAAVKVGTLIAERAQAKGISAVVFDRGGH
                     LYHGNVKALAEAAREKGLKF"
     gene            complement(1204..1761)
                     /gene="rplF"
                     /locus_tag="PLB01_09570"
     CDS             complement(1204..1761)
                     /gene="rplF"
                     /locus_tag="PLB01_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQQ77589.1"
                     /translation="MSRVGKKPVTVPKGVDVQVGASAVTVKGPKGSMTAPILAGIAVE
                     KKDGTVVLSRKGEEKQDKALHGLNRALLQNAVLGVTEGFKREIDLVGIGFKAEVKGKN
                     VVFSVGFSHQVTFPMPEGVTVNVAPDAKAKDVTHIIVTGVDKQKVGQTATEIRGIRPP
                     DPYKLKGFRFVGEVLKKKAGKATGK"
     gene            complement(1769..2167)
                     /gene="rpsH"
                     /locus_tag="PLB01_09575"
     CDS             complement(1769..2167)
                     /gene="rpsH"
                     /locus_tag="PLB01_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQQ77590.1"
                     /translation="MNVTDPVSDLLTRLRNAISARHEKADVPSSRFKVELARILKDEG
                     YIKNFKVMDDRGGSLLRLYLKYDDTGNPVIHGLSRSSKPGRRLYKGKNELPEVLGGLG
                     VAIVSTSQGLLTGNDAKKRGVGGEVVCTVW"
     gene            complement(2181..2366)
                     /locus_tag="PLB01_09580"
     CDS             complement(2181..2366)
                     /locus_tag="PLB01_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HQQ77591.1"
                     /translation="MARRAAIIKANSKLKYAIRKRNRCARCGRPRAFLRKFGMCRICF
                     RNLALAGYIPGVTKASW"
     gene            complement(2384..2926)
                     /gene="rplE"
                     /locus_tag="PLB01_09585"
     CDS             complement(2384..2926)
                     /gene="rplE"
                     /locus_tag="PLB01_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQQ77592.1"
                     /translation="MTARLSEKYKKEVVPVLKKEFGIENPMAVPKVEKVVLNMGIGEA
                     ISNAKLVDAAAEELTKIAGQRAVVTKAKKSIATFKLRAGMPIGCRVTLRGDRMYEFLD
                     RLMNVALPRVRDFRGVPTRAFDGRGNYTLGVKDHVIFPEIDPTKIDKSKGLNVTIVTT
                     AGSDDRARVLLRELGMPFGR"
     gene            complement(2930..3295)
                     /gene="rplX"
                     /locus_tag="PLB01_09590"
     CDS             complement(2930..3295)
                     /gene="rplX"
                     /locus_tag="PLB01_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQQ77593.1"
                     /translation="MKKKQPTYGGKRRIRKDDTVVVLTGKDRGAQGKVLRVIPDKGRA
                     IVEGVNKIKRATRANPQKNIKGGILEREAPIAISNLMLVDPEGGKPTRVGIRKEGAEW
                     VRYAKKSGATIGTLRAAGE"
     gene            complement(3298..3672)
                     /gene="rplN"
                     /locus_tag="PLB01_09595"
     CDS             complement(3298..3672)
                     /gene="rplN"
                     /locus_tag="PLB01_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQQ77594.1"
                     /translation="MIQMGTILEVADNSGARRLACIKVQGGSTGDYGRIGDVITCSVR
                     ESNPDAPDPVKKGKVVKAVIVRTVREHRRRDGSYIKFDTNAAVLVNNEKEPIGTRVFG
                     PVARELREKKFMKIISLAPEVI"
     gene            complement(3669..4052)
                     /gene="rpsQ"
                     /locus_tag="PLB01_09600"
     CDS             complement(3669..4052)
                     /gene="rpsQ"
                     /locus_tag="PLB01_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQQ77595.1"
                     /translation="MSTNPSKTDAPAGASAPARRSRKTGTVVSNKMEKTVVVEVSRLV
                     KHDRYGRYMKRSERFLADDRVLKSQPGDTVQIEETRPLSAKKRWRVIAIVTKGHGEVA
                     DTAEVPGSSTAKTVKRVPAPEGRAS"
     gene            complement(4081..4539)
                     /gene="rpmC"
                     /locus_tag="PLB01_09605"
     CDS             complement(4081..4539)
                     /gene="rpmC"
                     /locus_tag="PLB01_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQQ77596.1"
                     /translation="MKIQAMLDWSSDEKAAKVKEWTQSLFTLRLQKATGQLENPMKIK
                     QLRKDIARLKTAQHMGAAEVAAAAAHEVAPATTEGAEEKKPAAEPKAHAAASGKGAKK
                     RAAAAKSTGKKAAGAPAKAHSPSKKSAGPKGKTAGPSKAKSATPKKSKKK"
     gene            complement(4542..4967)
                     /gene="rplP"
                     /locus_tag="PLB01_09610"
     CDS             complement(4542..4967)
                     /gene="rplP"
                     /locus_tag="PLB01_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQQ77597.1"
                     /translation="MLMPSKVKYRKQQRGRMRGTALRGSELSFGEYGLQALEPGWLTA
                     RQIEAGRVAVQRHVKRGGKLWIRVFPDKPITKKPLETRMGKGKGAPEGWVCVVKPARI
                     LFEMEGVDEKTAREAFKLAADKIGIATRFVSRTFLSAVR"
     gene            complement(4980..5627)
                     /gene="rpsC"
                     /locus_tag="PLB01_09615"
     CDS             complement(4980..5627)
                     /gene="rpsC"
                     /locus_tag="PLB01_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQQ77598.1"
                     /translation="MGQKVHPYGFRIGYNRTWHSRWYAEKDYLKFLHDDIKLREELKR
                     DLANAAVSEVEIERGNKLKINIMTARPGVIIGKKGASVDQLRDRLQQRLGKDIHVNII
                     EIQRPETDAQLVAESIAMQLERRVAFRRAMKKGMESAFRFGAQGIKIRCGGRLNGAEI
                     ARSEWYQEGRLPLHTLKADIDYGFAEARTTYGKIGVKCWIYKGDLHRAKSRRKVA"
     gene            complement(5639..6238)
                     /locus_tag="PLB01_09620"
     CDS             complement(5639..6238)
                     /locus_tag="PLB01_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HQQ77599.1"
                     /translation="MESVARLRHYRGSAQKVRLVADLIRLKPVGKALATLRTTRKGCS
                     SDLLKLVESALANAQQNESGVDADALVVSKIHVDEAGRKPLQRNAMIRRISNYKKRFA
                     RPRFMSGPQGRMWLVPRPFCHVTVVLSDESMARKAAGLKAEAPKPLVKKRQGRVAAAV
                     SAAAARPKGAKAEHKHAEPKGEKGATKSKKPSAKPGAAE"
     gene            complement(6250..6585)
                     /gene="rpsS"
                     /locus_tag="PLB01_09625"
     CDS             complement(6250..6585)
                     /gene="rpsS"
                     /locus_tag="PLB01_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQQ77600.1"
                     /translation="MSRSTKKGPFVDAPLLEKIASMNEAGEKRVLKTWSRRSTILPDM
                     VGHTLAVHNGKKFIPVYISENMVGHKLGEFSPTRTFKGHSGRKVEKGATPGAPGAPGA
                     PAAAPAAKA"
     gene            complement(6601..7422)
                     /gene="rplB"
                     /locus_tag="PLB01_09630"
     CDS             complement(6601..7422)
                     /gene="rplB"
                     /locus_tag="PLB01_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQQ77601.1"
                     /translation="MPVKSYKPTSPGMRQRTTSDFSEITKSAPTKSLTKGKLKTGGRD
                     SRGHLTSWWRGGGHKKRYRDIDFKRDKHGIPAKVASVEYDPNRSSRIALLNYADGEKR
                     YIVAPAGLAVGATVVSGPTADIVVGNALPLKSIPLGTVVHNIEFQPGRGGQIARSAGA
                     SAQLMAKEGGFALLRVPSGELRRVRVDCYATIGQVGNLEHENVSIGKAGRNRWLGWKS
                     HNRGVAMNPVDHPMGGGEGKTSGGRHPTSPWGWKTKGKKTRNNKRTDALIVKRRK"
     gene            complement(7427..7726)
                     /gene="rplW"
                     /locus_tag="PLB01_09635"
     CDS             complement(7427..7726)
                     /gene="rplW"
                     /locus_tag="PLB01_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQQ77602.1"
                     /translation="MNPRTHQAVIVRPVLTERSTAMSQTQNVLTFDVARDANKIEIRK
                     AVEALFQVKVDAVRVVAVNGKIKKVGKSIGRAQDRKKAYVKLAKGQKLPALFEGV"
     gene            complement(7723..8427)
                     /gene="rplD"
                     /locus_tag="PLB01_09640"
     CDS             complement(7723..8427)
                     /gene="rplD"
                     /locus_tag="PLB01_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQQ77603.1"
                     /translation="MADKKTKTAAPKKAAAPKTAAPKAAGSSLKLKVRNVKAETVGEA
                     TLPEEIFGVPFKRHLVWEVVKEIRAREHRGTHKTKTRSEVSGSGRKPFKQKSTGRARQ
                     GGGRPPIHRHGGTVFGPVPHSYALKVNAKAKKAALRCVLSEKAREQKVVLLDALKLAT
                     HKTKDFAGVLAKLGVEGKALVVDRKDNENLARAVANNPKVTTLDPLGLNVYDALNAGT
                     IVLTEDALRAVSEVLA"
     gene            complement(8437..9090)
                     /gene="rplC"
                     /locus_tag="PLB01_09645"
     CDS             complement(8437..9090)
                     /gene="rplC"
                     /locus_tag="PLB01_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQQ77604.1"
                     /translation="MINGILGRKIGMTQVFDADGTVVPVTVVEAGPCLVVQRKTKDHD
                     GYDAVQLGLVGRVSPRRLTAAAKGHFEKAGVPPAKTLGEVRCDADEKAAPGDKVLCDI
                     FAEKEHVDVVGVSKGKGFQGVVKRHGFTGGAATHGSMFHRAPGSIGPSAFPSRVYPGT
                     RSSGRMGGKQTTMKNLVVVRVDTENHLIYLRGAVPGARNTVVKIVKKLSRKAGGAAA"
     gene            complement(9124..9441)
                     /gene="rpsJ"
                     /locus_tag="PLB01_09650"
     CDS             complement(9124..9441)
                     /gene="rpsJ"
                     /locus_tag="PLB01_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQQ77605.1"
                     /translation="MANDRIRIRLKGYDYRILDQSTTEIVDTARRSGAKVAGPIPLPT
                     AIARWTVNRSPHVDKKSREQFEMRTHKRLLDIFEWTPQTVDALTKLELPAGVEVEIRT
                     MGK"
     gene            complement(9548..10750)
                     /gene="tuf"
                     /locus_tag="PLB01_09655"
     CDS             complement(9548..10750)
                     /gene="tuf"
                     /locus_tag="PLB01_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HQQ77606.1"
                     /translation="MAKEKFERKKPHVNVGTIGHVDHGKTTLTAALTKVLATKGLAVS
                     KSYDDVAKADAKTFRRDATKVLTVALAHVEYETEKRHYAHIDCPGHADYIKNMITGAA
                     QMDGAVLVVSAPVGPMPQTKEHVLLARQVNVPAMVVYLNKCDLMEDPELLDLVELEVR
                     ELLSKYGFPGDKIPVIRGSAIKALNDPTGPDAKSIWELAEALDASIPEPQRATDKDFL
                     LPIEDVFSISGRGTVVTGRVERGVVKVGDEIEIVGLRDTQKKTVTGVEMFKKLLDQGQ
                     AGDNVGVLLRGVERTDVERGQVLCKPGSIKPHTKFKGKVYILSKDEGGRHTPFFNNYR
                     PQFFIRTTDVTGSAALPAGVEMVMPGDDVELEITLIAPIAMEKGLKFAIREGGRTVGA
                     GTVTEILA"
     gene            complement(10781..>12702)
                     /gene="fusA"
                     /locus_tag="PLB01_09660"
     CDS             complement(10781..>12702)
                     /gene="fusA"
                     /locus_tag="PLB01_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQQ77607.1"
                     /translation="MVQEQERGITITSAATTCHWKGIRINIIDTPGHVDFTAEVERSL
                     RVLDGAVAVFDAVSGVEPQSETVWRQADRYGVPRIAFINKMDRVGADFDRCVDMMVTK
                     LAAKPTPVQIPWGSAETLEGVIDLIEGKAYDFKDETLGAEFLTVDVPADHKAMYDKYR
                     ERLIEALAETDDALMQKYMDGHIPTPDEMRAALRKATVTGKLHPVVCGSAFKNKGVQQ
                     LLDAVVEYLPSPLDIPAIKGLDEDGKEIALDTRDDAPFAGLVFKIMTDPFVGQLAFFR
                     VYAGHLDAGSGVYNATKQTNERVGRLLKMHANKREEIKEIWAGDIGAAVGLKNVTTGD
                     TICDKNHVVVLESMKFPEPVITLSIEPKTKVDQEKMGMALAKLMQEDPTFRVHTDKET
                     NQTIIAGMGELHLEIIVDRMVREFNVGANVGKPQVAYRETIRRETPGEGKFVRQSGGK
                     GQYGHAKIRLRPHPEGKDYEFVNGITGGSIPKEFIKPIDQGIREAIQTGVLAGYPTVN
                     IQVELYDGSYHDVDSSEMAFKIAGSMAFQDAAKKANPVILEPMMAVEVVTPEDFMGDV
                     IGDLNSRRGKINHMEPRLNFQVITASVPLSEMFGYATTLRSLTQGRANYTMQFERYDE
                     VPKAIAEGIIAKVSV"
BASE COUNT         1629 a         4226 c         4341 g         2506 t
ORIGIN      
        1 gccggatgcg gcggaggccg aggccgaaca gcgtctgctt ctggtcgacg ggcgcgcaga
       61 tgcccgactt cacctggcgg attcggaggg atttcttgga aggcgcgtgc gcggccttcg
      121 cgtcggcacc ggcggctccg cgggactcgg gacccttcga agaaatcttc ttcttctctt
      181 cagccattag actttctccg tggcttccgt ggccggagcg gccgtcgcgg gttcggcggg
      241 cctggcctcg ccgttctcgc ggccgcgcgc cttcgcgatc gtctcctcga gctggaggtg
      301 caggagggcg tcgaacgtgg ccttcacgac gttgtgaggg ttcgtcgtcc cgagggactt
      361 cgtgaggatg ttctggactc cggcggactc gagcaccgcg cggaccgctc cgcccgcgat
      421 gacgcccgtg ccctcggagg ccggcttgag catgacgcgg cccgcgccgt agtggccgac
      481 gatcgggtgc gggatcgtct tgcccttgag cgggaccttg atcatcttct tcttggccat
      541 ctcgatcccc ttcttgatgg ccatggggac ttccttcgcc ttgccggcgc cgtagccgac
      601 gcggccgtgg ccgtcgccaa cgacgacgag cgcggagaag gagaagttct tgccgccctt
      661 gacgaccttc gtcacgcggt tgatgtgcac gacgtggtcg atgaggtcgg ctcccgcgcg
      721 gccctcaccg agggcgccgg ccggacgggg cggagggccg ccggggccgg agcggccgcc
      781 ggggcggttt ccacgcgggg gacgattgac gggcgcgttg ctgctcaaaa cttcaacccc
      841 ttctcgcgag cggcttccgc gagcgctttc acgttgccgt ggtagaggtg gccgccccgg
      901 tcgaagacga ccgcggaaat gcccttcgcc tgggcgcgct cggcaatgag cgttcccacc
      961 ttgacggcgg cgttcttgtt ggcggcgctc ttggccttgc cgcggacgcc ctcctcgcgg
     1021 gaggtcgcgt gcgcgagcgt cttgccggcg gcgtcgtcga tgatctggac ggagatccag
     1081 cggagggtct tgctgacggc gaggcgaggc cgctcggccg tccccttcac cttcgtgcgg
     1141 atgcgcgcgt ggatgcgcgt ccgcgcctcg acccgggcct gtgccttctg catggtcagt
     1201 tcctcacttg ccggtggcct tgccggcctt cttcttgagg acctcgccca cgaagcggaa
     1261 gcccttgagc ttgtacgggt ccggcggacg gatcccgcgg atctcggtcg ccgtctggcc
     1321 gaccttctgc ttgtcgacgc cggtcacgat gatgtgggtg acgtccttgg ccttcgcgtc
     1381 cggcgcgacg ttcaccgtga cgccctcggg catcgggaac gtcacctggt gcgagaagcc
     1441 gacgctgaac acgacgttct tccccttgac ctcggccttg aagccgatgc cgacgaggtc
     1501 gatttcccgc ttgaagcctt ccgtcacgcc gaggaccgcg ttctggagga gcgcgcggtt
     1561 caggccgtgg agggccttgt cctgcttctc ctcgcccttg cgcgacagga cgacggtccc
     1621 gtccttcttc tcgacggcga tccccgcgag gatcggcgcc gtcatggagc ccttcggccc
     1681 cttcacggtc acggccgagg cgccgacctg gacgtcgacg cccttgggca cggtcaccgg
     1741 cttttttccg actcgagaca tggcttcctc accacaccgt gcagacgacc tcgccgccga
     1801 cgccgcgctt tttcgcgtcg ttgccggtga ggaggccctg cgaagtggac acgatggcga
     1861 cgccgagtcc gccgaggacc tcgggaagct cgttcttgcc cttgtagaga cggcggccgg
     1921 gcttgctcga ccgcgagagg ccgtggatga ccgggttgcc cgtgtcgtcg tacttgaggt
     1981 acaggcgcag gaggcttccg ccgcggtcgt ccatgacctt gaagttcttg atgtagccct
     2041 cgtccttgag gatgcgggcg agctccacct tgaagcgcga cgacgggacg tcggccttct
     2101 cgtggcgcgc ggaaatcgcg ttcctcaggc gggtcaggag gtcggagacg ggatctgtga
     2161 cgttcatctc gccctcctcc ctaccacgac gccttcgtca cgcccgggat gtagccggcg
     2221 agcgcgagat tgcggaaaca gatgcggcac atgccgaact tgcggaggaa agcccgcgga
     2281 cgtccgcagc gcgcgcaacg gttgcgcttg cggatcgcgt acttcagctt cgagttcgcc
     2341 ttgatgatcg cggcgcgacg agccatttcg gtcgctcctt ggcctatcgc ccgaacggca
     2401 tgccgagttc ccgaaggaga acccgggccc ggtcatccga gccagccgtt gtgacgatgg
     2461 tgacgttgag tcccttggat ttgtcgatct tggtggggtc gatctcggga aagatcacgt
     2521 ggtccttcac gccgagggtg tagttcccgc ggccgtcgaa ggcgcgcgtc ggcacgccgc
     2581 ggaagtcgcg gacgcgcggc agcgcgacgt tcatgaggcg gtcgaggaac tcgtacatcc
     2641 ggtccccgcg cagcgtgacg cggcagccga tcggcatgcc ggcgcgcagc ttgaaggtcg
     2701 cgatggactt cttcgctttc gtcacgacgg cccgctggcc ggcgatcttc gtgagctcct
     2761 cggccgcggc gtcgacgagc ttggcgttcg agatcgcctc gccgatgccc atgttgagga
     2821 cgaccttttc caccttcggg accgccatcg ggttctcgat gccgaattcc ttcttgagga
     2881 cggggacgac ctctttcttg tacttctcgg aaagtcgcgc ggtcatggct tactcccccg
     2941 ccgcccggag cgtcccgatc gtggcgccgc tcttcttggc gtagcggacc cactcggcgc
     3001 cctccttgcg gatgccgacg cgcgtcggct tgccgccctc ggggtcgacg agcatgaggt
     3061 tcgagatggc gatcggagcc tcgcgctcga ggatgccgcc cttgatgttc ttctgggggt
     3121 tcgcccgcgt cgcgcgcttg atcttgttca cgccctcgac gatggcccgg cccttgtccg
     3181 ggatgacccg caggaccttg ccctgcgcgc cccggtcctt gccggtgagg acgacgacgg
     3241 tgtcgtcctt gcggatgcgg cgcttgccgc cgtacgtcgg ctgtttcttc ttcatggtca
     3301 gatgacttcc ggagccagcg agatgatctt catgaacttc ttctcgcgga gctcgcgcgc
     3361 gaccggcccg aagacgcggg tgccgatcgg ctctttttcg ttgttgacga ggacggccgc
     3421 gttcgtgtcg aacttgatgt agctgccgtc ccggcggcgg tgctcgcgca ccgtgcggac
     3481 gatgacggcc ttgacgacct tccccttctt gaccgggtcc ggcgcgtccg ggttcgactc
     3541 ccggaccgaa caggtgatga cgtccccgat gcggccgtaa tcgccggtcg agccgccctg
     3601 gaccttgatg caggccaggc gccgcgctcc ggagttgtcg gcgacctcga ggatcgttcc
     3661 catctggatc acgacgccct cccttcaggc gcggggaccc gcttgaccgt cttggcggtc
     3721 gagctgcccg ggacctcggc cgtgtccgcg acctctccgt ggcccttcgt gacgatcgcg
     3781 atgacgcgcc agcgcttctt cgccgacagc gggcgggtct cctcgatctg gaccgtgtcg
     3841 cccggctggg acttcagcac ccggtcgtcc gcgaggaaac gctcggagcg cttcatgtag
     3901 cggccgtacc gatcgtgctt gacgaggcgg gacacctcga cgacgacggt cttctccatc
     3961 ttgttcgaga cgacggttcc ggtcttgcgc gagcgccgcg cgggcgcgga cgcgcccgcc
     4021 ggggcgtcgg tcttgctcgg gttcgtgctc atgggcgtgc tcactgcttc ctgttaccga
     4081 ctacttcttc ttgctcttct tcggcgtggc ggacttggcc ttcgaggggc cagccgtctt
     4141 gcccttcggt cccgcagatt tcttagaagg tgaatgggcc ttcgccgggg cgccggcggc
     4201 cttcttgccg gtggacttcg cggccgccgc gcgcttcttc gcgcccttgc ccgacgccgc
     4261 ggcgtgcgcc ttcggctccg ccgccggctt cttctcctcg gctccctcgg tcgtcgccgg
     4321 cgcgacttcg tgcgccgcgg ccgccgccac ctcggccgcg cccatgtgct gggcggtctt
     4381 gaggcgggcg atgtccttgc ggagctgctt gatcttcatc gggttctcga gctggcccgt
     4441 cgccttctgg agacgcagcg tgaagaggga ctgcgtccac tccttcacct tcgcggcctt
     4501 ctcgtcggag gaccagtcga gcatcgcctg gatcttcatg ctcacctcac cgccgaaagg
     4561 aacgtgcgcg agacgaaacg cgtcgcgatc ccgatcttgt ccgccgcgag cttgaacgcc
     4621 tcgcgcgcgg tcttctcgtc cacgccttcc atctcgaaca ggatccgggc cggcttgacg
     4681 acgcagaccc agccttcggg cgcgcccttg cccttgccca ttcgggtctc gagcggcttc
     4741 ttcgtgatcg gcttgtcggg gaacacgcgg atccacagct ttccgccgcg cttgacgtgg
     4801 cgctggaccg cgacgcggcc cgcctcgatc tggcgcgccg tgagccagcc cggctcgagc
     4861 gcctgcagcc cgtactcccc gaaggagagt tcggacccgc ggagcgccgt gccgcgcatg
     4921 cggccgcgct gctgtttgcg gtacttgacc tttgacggca tcaacatggg cgttcttcct
     4981 tacgcaacct tccggcggct cttcgcgcga tgcaggtcgc ccttgtagat ccagcacttc
     5041 acgccgatct ttccgtacgt cgtccgcgcc tcggcgaacc cgtagtcgat gtcggccttg
     5101 agggtgtgga gcgggaggcg gccttcctgg taccactcgg agcgcgcgat ctcggcgccg
     5161 ttcaggcggc cgccgcagcg gatcttgatt ccctgtgcgc cgaagcggaa ggcggactcc
     5221 atgcccttct tcatcgcgcg gcggaacgcg acgcggcgct cgagctgcat cgcgatggac
     5281 tcggcgacga gctgggcgtc cgtctcgggg cgctggatct cgatgatgtt cacgtggatg
     5341 tccttgccga gccgctgctg cagccggtcg cggagctggt ccacggacgc gcccttcttc
     5401 ccgatgatga cgccgggacg ggccgtcatg atgttgatct tgagcttgtt cccgcgctcg
     5461 atctcgacct cggacaccgc ggcgttcgcg aggtcgcgct tgagctcctc gcggagcttg
     5521 atgtcgtcgt ggaggaactt gaggtagtcc ttctcggcgt accagcgcga atgccaggtc
     5581 cggttgtagc cgatgcggaa accgtagggg tggactttct gacccatcgt tgactccgtt
     5641 actcggccgc gcccggcttg gcggacggct tcttggactt ggtggcgccc ttttcgccct
     5701 tcggctcggc gtgcttgtgc tcggccttgg cgcccttcgg acgcgcggcg gcggccgaca
     5761 cggcggccgc gacgcggccc tgacgcttct tgacgagcgg cttcggcgcc tcggccttga
     5821 ggccggcggc cttgcgggcc atcgactcgt cggagaggac gacggtgacg tggcagaacg
     5881 ggcgcgggac gagccacatg cggccctgcg ggccggacat gaagcgcggg cgcgcgaacc
     5941 gcttcttgta gttcgagatc ctgcggatca tcgcgttgcg ctgcaggggc ttccttccgg
     6001 cctcgtcgac gtggatcttc gagacgacga gcgcgtccgc gtcgacgccc gactcgttct
     6061 gctgggcgtt cgcgagcgcg gactccacga gcttcaggag atcggacgag cagcccttgc
     6121 gcgtcgtgcg cagggtcgcg agggccttcc ccacgggctt gagccggatc aggtccgcca
     6181 cgaggcggac cttctgcgcg gagccgcggt agtggcggag acgggcgacg gattccatca
     6241 gtcccctcct caggccttcg ccgccggcgc ggcggccggt gcgccaggcg cgcccggagc
     6301 gccgggcgtg gcgcccttct cgaccttgcg gcccgagtgg cccttgaacg tgcgcgtggg
     6361 cgagaactcg ccgagcttgt gcccgaccat gttctcggag atgtagaccg ggatgaactt
     6421 cttgccgttg tggacggcca gcgtgtggcc gaccatgtcg ggcaggatcg tcgagcggcg
     6481 cgaccacgtc ttgaggacgc gcttttcccc cgcttcgttc atcgaggcga tcttttcgag
     6541 gagcggcgcg tcgacgaacg gccccttttt cgtggaacga gacatgtttt ccctcgtctc
     6601 ctacttgcgg cgcttgacga tgagcgcgtc ggtacgcttg ttgttgcggg tcttcttgcc
     6661 cttggtcttc cagccccacg gcgacgtggg gtggcggccg cccgaggtct tgccctcgcc
     6721 gccgcccatc gggtggtcga cggggttcat cgcgacgccg cggttgtgcg acttccatcc
     6781 gagccagcgg ttgcggccgg ccttgccgat cgagacgttc tcgtgctcga ggttgccgac
     6841 ctggccgatc gtggcgtagc agtccacacg aacgcggcgc agctcgcccg agggcacgcg
     6901 cagcagggcg aacccgccct ccttcgccat gagctgggcg ctcgcgccgg ccgaacgggc
     6961 gatctgcccg ccgcggcccg gctggaactc gatgttgtgc acgacggtgc cgagcgggat
     7021 cgacttgagg ggaagcgcgt tcccgacgac gatgtcggcc gtcgggcccg acacgacggt
     7081 cgccccgacc gcgaggccgg ccggcgcgac gatgtaacgc ttctcgccgt cggcgtagtt
     7141 caggagcgcg atgcgggacg agcggttcgg gtcgtactcg acggaggcga ccttcgccgg
     7201 gatcccgtgc ttgtcgcgct tgaagtcgat gtcgcggtac cgcttcttgt ggcctccgcc
     7261 gcgccaccag gacgtgaggt ggccacggct gtcgcggccg cccgtcttca gcttgccctt
     7321 cgtgagggac ttcgtcggcg cggacttcgt gatctccgag aaatcggagg tcgtccgctg
     7381 gcgcatgccg ggagacgtcg gtttgtagga cttcacgggc atggcgtcac accccttcga
     7441 acagggccgg gagcttctgg cccttcgcga gcttcacgta ggccttcttg cggtcctgcg
     7501 cgcgcccgat ggacttcccg accttcttga tcttcccgtt cacggcgacg acgcggacgg
     7561 cgtcgacctt gacctggaag agggcctcga cggccttgcg gatctcgatc ttgttcgcgt
     7621 cgcgggcgac gtcgaacgtg aggacgttct gggtctggct catcgccgtc gagcgctcag
     7681 tcaggacggg gcgcacgatg acggcctggt gagtgcgcgg gttcatgcca gcacctccga
     7741 gacggcgcgg agggcgtcct cggtgaggac gatcgttccg gcgttcagcg cgtcgtagac
     7801 gttgagcccg agcgggtcca gcgtcgtgac cttgggattg ttcgcgacgg cgcgggcgag
     7861 gttctcgttg tccttgcggt cgacgacgag cgccttgccc tcgaccccga gcttcgcgag
     7921 cacgcccgca aagtccttgg tcttgtgcgt cgcgagcttg agcgcgtcga gaagcacgac
     7981 cttctgctcg cgcgccttct cggacagcac gcagcgcagc gcggccttct tcgccttcgc
     8041 gttcaccttg agcgcgtacg agtgcgggac cggaccgaac accgtcccgc cgtggcggtg
     8101 gatcggcggg cgtccgccgc cctgccgggc gcggcccgtg ctcttctgct tgaagggctt
     8161 cctgcccgag ccggacacct cggagcgggt cttcgtcttg tgggttccgc ggtgctcgcg
     8221 cgcgcggatc tccttgacga cttcccacac gagatggcgc ttgaacggca cgccgaagat
     8281 ctcctcgggg agcgtcgcct cgccgacggt ctcggccttg acgttgcgca ccttgagctt
     8341 cagcgaggag ccggcggcct tcggagccgc agtcttcggg gcggcggcct tcttcggagc
     8401 ggcggtcttc gtcttcttgt ctgccatggc cgctccttac gccgccgctc cgccggcctt
     8461 gcgcgagagc ttcttcacga tcttgacgac cgtgttgcga gctcccggca cggcgccgcg
     8521 gagatagatg aggtggttct cggtgtccac gcggacgacg acgaggttct tcatcgtcgt
     8581 ctgcttgccg cccatgcgtc ccgacgagcg cgtgcccggg tagacgcggg aggggaaggc
     8641 cgacgggccg atggagcccg gcgcgcggtg gaacatcgag ccgtgcgtcg cagcgccgcc
     8701 cgtgaagccg tggcgcttca cgacgccctg gaagcccttg cccttggaga cgccgacaac
     8761 gtcgacgtgc tccttctcgg caaagatgtc acagagcacc ttgtcgccgg gggcggcctt
     8821 ctcgtcggcg tcgcagcgaa cctcgccgag ggtcttcgcc ggcggcacgc ccgccttctc
     8881 gaaatggccc ttggcggccg ccgtgaggcg gcgcggcgac acgcggccga cgagcccgag
     8941 ctggacggcg tcgtacccgt cgtggtcctt cgtcttgcgc tgaacgacca ggcaggggcc
     9001 ggcctcgacg accgtgaccg gcaccaccgt gccgtcggcg tcgaagacct gcgtcatgcc
     9061 gatcttgcgt cccaggattc cgttaatcat gtttcgttcc tcgtctcgtc tgtgacggga
     9121 cggttacttc cccatcgtcc ggatttcgac ctcgacgccc gcgggcagct cgagcttcgt
     9181 gagggcgtcg acggtctgcg gcgtccactc gaagatgtcg agcaggcgct tgtgggtccg
     9241 catctcgaac tgctcgcgcg acttcttgtc gacgtgcggc gagcggttga ccgtccagcg
     9301 ggcgatggcc gtcggcagcg gaatcggtcc cgcgaccttc gcgccggagc gccgcgccgt
     9361 gtcgacgatc tcggtcgtcg actggtcgag gatccggtag tcgtaaccct tgaggcggat
     9421 gcggattctg tcgttggcca ttggcgtgtg ctttctttcg agcgagttca ggccgccgga
     9481 gaggaccggg ggatttcttc gaaggtgaag agcccccgga ccacaacggc gacgatctct
     9541 ctatctctta agcaagaatc tccgtaacgg tgcccgcgcc gaccgtgcgg ccgccctcgc
     9601 ggatggcgaa cttgaggccc ttctccatgg cgatcggcgc gatgagcgtg atctcgagct
     9661 cgacgtcgtc gccgggcatg accatctcga cgccggccgg cagagcggcc gagcccgtga
     9721 cgtccgtcgt gcggatgaag aactgcgggc ggtagttgtt gaagaacggc gtgtggcggc
     9781 cgccctcgtc cttggacagg atgtacacct tgcccttgaa cttcgtgtgc ggcttgatcg
     9841 agccgggctt gcagaggacc tgcccgcgct cgacgtccgt gcgctcgacg ccgcggagca
     9901 ggacgccgac gttgtcgccc gcctggccct ggtcgaggag cttcttgaac atctcgacgc
     9961 ccgtgaccgt cttcttctgg gtgtcgcgca ggccgacgat ctcgatttcg tctccgacct
    10021 tcacgacgcc gcgctcgacg cggcccgtga cgaccgtgcc gcggcccgag atcgagaaca
    10081 cgtcctcgat cgggaggagg aagtccttgt ccgtggcgcg ctggggctcg gggatgctcg
    10141 cgtcgagggc ctcggcgagc tcccagatgc tcttggcgtc ggggccggtc gggtcgttga
    10201 gcgccttgat ggccgagccg cggatcaccg ggatcttgtc gccggggaag ccgtacttgc
    10261 tgagaagctc gcgcacctcg agctcgacga ggtcgaggag ctcgggatct tccatgaggt
    10321 cgcacttgtt gaggtagacg accatcgcgg gcacgttgac ctgccgggcg agaaggacgt
    10381 gctccttcgt ctgcggcatc gggccgaccg gcgccgagac gacgaggacc gcgccgtcca
    10441 tctgcgcggc gcccgtgatc atgttcttga tgtagtccgc gtggcccggg cagtcgatgt
    10501 gggcgtagtg ccgcttctcg gtctcgtact cgacgtgcgc gagggcgacc gtgaggacct
    10561 tggtcgcgtc gcgccggaac gtcttcgcgt ccgccttggc gacgtcgtcg taagacttgg
    10621 agacggcgag gcccttcgtc gcgaggacct tcgtcagagc cgccgtcagg gtcgtcttgc
    10681 cgtggtcgac gtgaccgatc gtgccgacgt tgacgtgggg cttcttgcgc tcgaatttct
    10741 ccttggccat ctgtctctcc ttcgctcctg gcggctttct ctagaccgaa accttggcga
    10801 tgatcccctc ggcgatcgcc ttcgggacct cgtcgtatct ctcgaactgc atcgtgtagt
    10861 tggcgcggcc ctgggtgagg gaccgcaggg tggtggcgta gccgaacatc tcggacagcg
    10921 ggacgctggc cgtgatgacc tggaagttca ggcgcggctc catgtggttg atctttccgc
    10981 gccgcgagtt gaggtcgccg atgacgtcgc ccatgaagtc ctcgggcgtg acgacctcga
    11041 cggccatcat cggctcgagg atgaccgggt tcgccttctt cgcggcgtcc tggaacgcca
    11101 tcgagcccgc gatcttgaag gccatttccg acgagtcgac gtcgtggtac gagccgtcgt
    11161 acagctcgac ctggatgttg accgtcgggt aaccggcgag cacgcccgtc tggatcgcct
    11221 cgcggatgcc ctggtcgatc ggcttgatga attccttggg gatcgagccg cccgtgatgc
    11281 cgttgacgaa ctcgtagtcc ttgccttccg ggtggggacg caggcggatc ttcgcgtggc
    11341 cgtactggcc cttgccgccc gactggcgca cgaacttgcc ctcgcccggc gtctcgcgcc
    11401 ggatcgtctc gcggtacgcg acctggggct tgcccacgtt cgcgcccacg ttgaactcgc
    11461 gcaccatgcg gtcgacgatg atctcgaggt ggagctcgcc catcccggcg atgatcgtct
    11521 ggttcgtctc cttgtccgtg tggacgcgga acgtcgggtc ttcctgcatg agcttcgcga
    11581 gcgccatgcc catcttttcc tggtcgacct tcgtcttcgg ctcgatcgag agcgtgatga
    11641 cgggctcggg gaacttcatg gactcgagca cgacgacgtg gttcttgtcg cagatcgtgt
    11701 cgccggtcgt gacgttcttg aggccgacgg cggcgccgat gtcgcccgcc cagatctcct
    11761 tgatctcctc gcgcttgttc gcgtgcatct tgaggaggcg gccgacgcgc tcgtttgtct
    11821 gcttcgtggc gttgtagacg cccgacccgg cgtcgaggtg cccggcgtag acgcggaaga
    11881 acgcgagctg gccgacgaac gggtcggtca tgatcttgaa gacgaggccc gcgaacggcg
    11941 cgtcgtcgcg cgtgtcgagc gcgatctcct tgccgtcctc gtcgaggccc ttgatcgccg
    12001 ggatgtcgag gggcgagggc aggtactcga cgacggcgtc gaggagctgc tggacgccct
    12061 tgttcttgaa cgccgatccg cagacgaccg gatggagctt tcccgtgacg gtcgccttgc
    12121 gcagcgccgc gcgcatctcg tccggcgtcg ggatgtgccc gtccatgtac ttctgcatga
    12181 gggcatcgtc ggtctccgcc agcgcctcga tgaggcgctc gcggtacttg tcgtacatgg
    12241 ccttgtggtc ggccgggacg tcgacggtca ggaactcggc cccgagcgtc tcgtccttga
    12301 agtcgtaggc cttgccctcg atcaggtcga tgacgccctc gagcgtctcg gccgaacccc
    12361 acgggatctg caccggcgtg ggcttcgcgg cgagcttcgt gaccatcatg tcgacgcaac
    12421 ggtcgaagtc cgcgccgacg cggtccatct tgttgatgaa cgcgatgcgc gggacgccgt
    12481 agcggtccgc ctggcgccag acggtctccg actgcggctc gacgccggaa acggcgtcga
    12541 agacggcgac ggcgccgtcc aggacgcgca gcgagcgctc gacctcggcc gtgaagtcga
    12601 cgtggcccgg cgtgtcgatg atgttgatgc ggatgccctt ccagtggcag gtcgtcgcgg
    12661 ccgacgtgat cgtgatgccg cgctcctgct cctggaccat cc
//
LOCUS       DAOMFY010000097        12532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1556_length_12532_cov_8.185061, whole genome shotgun sequence.
ACCESSION   DAOMFY010000097 DAOMFY010000000
VERSION     DAOMFY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1556_length_12532_cov_8.185061"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="PLB01_09665"
     CDS             <1..1376
                     /locus_tag="PLB01_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77608.1"
                     /translation="AARSIRGPGGGMSEGAAALEPLEPARLKEFFLDGLVADWTRVST
                     APGATHYPYLPSLTPGRVAWLLVLIGLAQRGPLRIASAVLASAGVLLAVAGATPAFHF
                     AVKTLPFFASLRYPERHAALAGFGLATLVALGLARLDAALSPRARRVVLPLLALAVLV
                     DREPMARGLSPVEDGSVLTRPPALLERWEPAGGDAPPPRIFHRDLYAPVPVYDTQDLA
                     ASSVTARASLLPAYGSLFGAGYVFDKDYDLSLSVEVFEWMRLFGRAVPTPDPLPLRIV
                     RAAGASAVLVSERGADGRYRPRLLRVADPVPPFRFASRVVASDDARAVFKRFLEEGAD
                     AGTAYVDAALPGFPDAPGGGRILRLGDRPSGLDLDVEVDGPGEGFLMLWRLREAVEDA
                     TLDGRPASAVPMAFGFAGVRVPPGRHVLALRPETRWVKIGALVTAATAAALALAWAIR
                     RRGSAAA"
     gene            1373..2806
                     /locus_tag="PLB01_09670"
     CDS             1373..2806
                     /locus_tag="PLB01_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ77609.1"
                     /translation="MSPLSRNTRRRLVLYGGAAAVAAAVWLLPRILARREPGVPVRRL
                     DLEAASAMERPEVKLLVEVLRLDSSNPPGITRPVIDVFARELACAGIPPVVTGRDPQR
                     PILVARLPGRRRGEALALLAHADVVPAGNLSDWVKPPFAAERGDKEYASNIIARGALD
                     MKAQAVAHLLALTALARSGIAPERDIVFVMESGEESYDQTLGFGWLLANRPDLVAGVT
                     DVFNEGGVNEVNTTEIERFGVEVMQKAILSVDVLAAEKGPLEDLAAVVKARDEAEPLK
                     VVPAVRDFLRFIAPSRSDVWGRRMLDDPAKIGPGTEFWKVVPEVYRSLLKDMAYTGRI
                     AERPEGGVSMRVVRTLLPGSSVAAAEAELDGWLLARHLTKRIVLKTDDCVATPAEGPA
                     WRAVTEVFGLDPAHVPVGPYILSGQYTSSSFLRARGLRAFGVSPFAVGYFDATTIHHH
                     SERIVVPFYLEGLERMKRILFEFATAP"
     gene            2905..3183
                     /locus_tag="PLB01_09675"
     CDS             2905..3183
                     /locus_tag="PLB01_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77610.1"
                     /translation="MLEKSKSDVRDERPARSRPLTDADAKALLKEVDEVTLARGKTAR
                     TLPAREVSLDDLRGPTGNFRAPMLRVGRRLLVGWHEESLRELLARSSR"
     gene            3233..3790
                     /locus_tag="PLB01_09680"
     CDS             3233..3790
                     /locus_tag="PLB01_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77611.1"
                     /translation="MKTTLVTCAVLLAALPAAAQVQVQTQQVGPGPGIAAPQQPSPIP
                     CPGMGGLEVGQTRTFAFERMAKLEPGKAKVTRDVKVVHKPEGRPWVITATFDSDAADA
                     KVLGLYYYVDPPSGIGDALRERYGKGTAVPSAADQTYWDVGSCDVRLKYRARMNEKQR
                     PIEELWVDPIPKPAPSKAAPAKKKG"
     gene            3887..6160
                     /locus_tag="PLB01_09685"
     CDS             3887..6160
                     /locus_tag="PLB01_09685"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQQ77612.1"
                     /translation="MKRVLKAVALALALAILVAAGALLFLIRRNLPSSSSPKILGLSA
                     KVSVSFDDRGVATVTAATVRDALRAQGFLTARERLFQLELQRRLARGALAEVFGASLL
                     PADRLHRTYGFARVAEAAVPLLPEGERADLEALADGINAFLDARPGRLGIEFTLLGLS
                     PRRFTAADALAVHLLMCEDLSTSWRRDLALEKLSGAAPSVRRFVADTTSPSDVLLLPD
                     SAPLVAPPLPDLGGVPLAGLGFGTLREDEPRGSNAWAVAGILAKSGKPLFANDPHLAL
                     TLPGIWLPMRFVIGGRLVEGVTLPGLPGVILGRNERVAWGMTTLRTDVQDLYRETIVG
                     KRVKRGAGWEDVRERTETIAVRGGKPETLVVRETSHGPLVQGRMALKWLALDPSLMRV
                     PESAVMTATDRESLSRALDAFPYPAHNVVWAEAAGSIGWRATGLVPIRRTGTDGSVPY
                     DGADPENDWKGYVPPSDMPRVVNPPEGFVVTANQRDIGTRFPQTVTTDWPSTERARRI
                     ADLLGEAARGKRLLDRAAMEALQLDDVSPVLAATLSALAPELPEPWNARFAGWDGRAD
                     ADSTLFLVARSTRRKLVERVLAAWRVPPEVGLPDARLLELARADDAAFRRALLGPKRD
                     LVRAAFEASRADLTARFGRGEARWTWGASNRLAVKHPLGRLPGLGGLFDPPSFPQSGA
                     GGTPRVATPTYGQSMRFVVDWGAPDEATLVIPFGVSGHLGSAHRTDQLAAWREGDPGG
                     ARTRLARPAVTSLEITP"
     gene            6157..6990
                     /locus_tag="PLB01_09690"
     CDS             6157..6990
                     /locus_tag="PLB01_09690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQQ77613.1"
                     /translation="MTGAGALLAAVLSVASAPRTIPQGGVAEIRIASDAPLPELVLVD
                     GDRRVPLERDASGRKFSGLVGVDLDAKTGLHDFVVEAPDGAARLVLALKVLRGSFRIQ
                     KLTVDPKFVEVPPEEKERVKADGERVNAAYRSAEAERRWTAFVRPVAYDGTNFGSRRV
                     YNGKTQSFHAGLDMPAPAGTPVAAAADGRVALAGDLYFSGGTVLLDHGGGLFTQYMHL
                     SRVDVKDGDVVKKGAIVGLAGATGRVTGPHLHWGARWNGARVNPLALLALPARPAPGL
                     H"
     gene            6994..7404
                     /locus_tag="PLB01_09695"
     CDS             6994..7404
                     /locus_tag="PLB01_09695"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQQ77614.1"
                     /translation="MSVLITGRLVSPTATELDHGPSHKRLPTQAPKDNGGDGSSFSPT
                     DLCAASLGSCAATTLSLYATRAGIPLTGVTFSVEKHMTSEPPRRIARLVLELTIDSPC
                     GDEDFGKLVNAAKSCPVRRSLHPDVVVDETYVRA"
     gene            complement(7409..7552)
                     /locus_tag="PLB01_09700"
     CDS             complement(7409..7552)
                     /locus_tag="PLB01_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77615.1"
                     /translation="MARLLMDRGLRLVRPLEGGLDAWAEAGFEVEAERIPLSPTGTWT
                     PAG"
     gene            complement(7577..8383)
                     /locus_tag="PLB01_09705"
     CDS             complement(7577..8383)
                     /locus_tag="PLB01_09705"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQQ77616.1"
                     /translation="MKDLFALVLKHGTSLVFGNVLLEQLGLPVPAVPTLVVAGALAAD
                     GKLSFAHLLLAAFVATVAADSLWFLLGRRYGQSLLKTLCRVSLSPDTCVRQTEGMFEK
                     YGLASLLFAKFVPGYSTVAPPLAGASGTGYPRFLLFTSGGTLLWAGSALLLGVVFHGA
                     VDRVLALLSNMGGWAFVVLAGGLLLYILFKWHQRQRFYRFLRMARIRVADLRSLMDAG
                     EAPVVVDVRSRNARLRDPRRIPGAVTIDLEDLDAHVALLPADREIVLYCT"
     gene            complement(8391..8954)
                     /locus_tag="PLB01_09710"
     CDS             complement(8391..8954)
                     /locus_tag="PLB01_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQQ77617.1"
                     /translation="MVETLTAPYLLLAGPGLADPNFSKTVVLMGHHTKDGALGWVVNR
                     LLGQPAVTLLPPPLDATLHPATPLHIGGPVLTNGLVALFREEVDGVDCNEMAPGLWVS
                     ASAEILPKLFGKPPGDGPPQGLLVLGYAGWDAEQLEGEMEEGAWLVLPWEPDLAFTRR
                     VETLWERALARLGVDPGSVSSSSTGVS"
     gene            9003..9275
                     /locus_tag="PLB01_09715"
     CDS             9003..9275
                     /locus_tag="PLB01_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77618.1"
                     /translation="MTVPGSGLLLLAVASVVVLLVLLEAWRQRRIYGKPSGRPNLAGA
                     GMLELQKHLQADRRVEKLVEIQRDESAETEQDEAGGPRQPPARPPR"
     gene            complement(9272..10156)
                     /gene="bla"
                     /locus_tag="PLB01_09720"
     CDS             complement(9272..10156)
                     /gene="bla"
                     /locus_tag="PLB01_09720"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HQQ77619.1"
                     /translation="MILVLPVVLFAFASAAPAPDPVPDHPAVAAARAAEAGGLVAGVS
                     YRHLESGRAASWRGTETFQMASVFKLPVAIAVLDAVEREKLRLDGEVEIREKDRMEVG
                     PIYDEWKPGMRVTIARMVDVMLVDSDNTAADVLVAQLGGPAAVEKALVAKGVTGVKVS
                     LDERGLGAAMRMDLDAIERGAQNGTSPDAMTGLLVRLFRGELLSKDSTDRILDSMRRC
                     ATSGRRFRAGLPKGAEVFDKTGTMRLSSNDVGILTLRDGSHAVLAVFTRGGAGAEARE
                     KAIASIAKAAWEAGAPAR"
     gene            complement(10310..12256)
                     /gene="speA"
                     /locus_tag="PLB01_09725"
     CDS             complement(10310..12256)
                     /gene="speA"
                     /locus_tag="PLB01_09725"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HQQ77620.1"
                     /translation="MTPTALARASKKPARARAPERFADAEKLYGIENWGKGFFSVSDD
                     GNLLVHPTKENHRFVDLKGVVDDVALRGISTPVVIRFPQILDQAVKELNEAFLHAIKE
                     YDYDGVFRGVFPIKVNQKKVVVREIIQSGRKYGYGLEAGSKPELIAALSQDLGSDCLI
                     TTNGYKDDAFIRLALNGIRMGKTVVLILEKVSELEKILDVAKKRGVRPLIGMRSKLYA
                     RGSGKWAKSGGEAAKFGLTTTEMLEAVEILKQRKMLDSLVMLHFHIGSQITDIRKIKQ
                     AIKEAGRVYAKLHATGVEIQYLNLGGGLGVDYDGSKTAFDSSMNYTVQEYANDIVYTI
                     KSICDEEKVPVPTLVTESGRALTAYHSVLVTNVLDVADRIEQGRSVKLDGDENHVVRE
                     LYDIWQGVTAKNVRESYHDALQYKEELFTLFNLGYVSLEDRSKGEILYWNICDRIRRY
                     LHTLKDIPEEFEQLEVMLADKYVMNFSVFQSLPDVWAIDQLFPILPIHRLRERPSEHG
                     TLADITCDSDGKIEKFIDLRDIKEALPLHAFHKGTPYYIGFCLVGAYQDVLGDLHNLF
                     GEVHEVLASVDDDGRTRIQDVLPGESCERVLSYMNYDKDELLDGIWRQLRRATERKKV
                     KEPEAKAIAKDFEHSLTHYTYLEE"
     gene            12315..>12532
                     /locus_tag="PLB01_09730"
     CDS             12315..>12532
                     /locus_tag="PLB01_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HQQ77621.1"
                     /translation="MAAPLDLTLARRWGLPAGATAWRITTDLQADIVAFSQNRGLFAG
                     LSLQGSVLNQRSDWNRSYYGREMSPQQIV"
BASE COUNT         1732 a         4438 c         4439 g         1923 t
ORIGIN      
        1 cggccgcgcg cagcatccgt ggtcccggcg gcggaatgtc cgagggcgcc gcggcgctcg
       61 agccgctcga gcccgcgcgt ctgaaagagt tcttcctgga cggactcgtg gcggactgga
      121 cgcgcgtctc caccgccccc ggcgccaccc actacccgta cctcccgtcg ctgactccgg
      181 ggcgcgtcgc gtggctcctc gtccttatcg gcctcgcgca gcgcggcccg ctgcgcatcg
      241 cctcggccgt tctcgcgtcc gcgggagttc tcctcgccgt ggcgggcgcg acgccggcgt
      301 tccacttcgc ggtgaagacg ttgccgttct tcgcgtcgct ccgctatccg gaacgccacg
      361 cggcgctcgc gggcttcggg ctcgcgacgc tcgtggcgct cgggctcgcg cggctcgacg
      421 ccgcgctttc cccgcgggcg cggcgcgtcg tcctgcctct cctcgcgctc gcggtgctcg
      481 tcgaccgcga gccgatggcc cgcggcctct cgcccgtgga ggacggttcc gtcctgaccc
      541 ggcccccggc gctcctcgaa cgatgggagc cggccggcgg cgacgctccg ccgccgcgca
      601 tcttccaccg tgacctctac gcgcccgttc cggtctacga cacgcaggac ctcgccgcgt
      661 cgagcgtcac ggcgcgcgcg tcgctgctgc ccgcgtacgg ctcgctcttc ggcgccggct
      721 acgtcttcga caaggactac gacctgtcgc tgtcggtcga agtcttcgag tggatgcggc
      781 tcttcggccg cgcggtgccg acgccggacc ccctgccgct ccggatcgtc cgcgccgcgg
      841 gcgcttccgc ggtcctcgtg agcgaacggg gagcggacgg gcgataccgg ccgcgcctcc
      901 ttcgggtcgc ggacccggtg ccgccgttcc ggttcgcgtc ccgcgtcgtg gcctcggacg
      961 acgcccgggc cgtcttcaag cggttcctcg aggaaggcgc cgacgcgggg acggcgtacg
     1021 tcgacgcggc gctgccgggt tttccggacg cgcccggggg cggccgcatc ctcaggctgg
     1081 gcgaccggcc ctccgggctc gacctggacg tcgaggtcga cggcccgggc gaggggttcc
     1141 tcatgctctg gcgcctccgc gaggcggtcg aggacgcgac gctcgacggc cggccggcct
     1201 ccgcggtccc aatggcattc ggcttcgcgg gggtccgcgt cccacccggc cggcacgtcc
     1261 tcgcgttgcg gccggagacg cggtgggtga agataggggc gttggtcacc gcggcgacgg
     1321 ctgccgccct cgcgctcgcg tgggcgattc gccgtcgcgg ttccgccgct gcatgagccc
     1381 gctttcacgc aacacccggc gccgcctggt gctctacggc ggtgccgccg ccgtcgccgc
     1441 ggccgtctgg ctcctcccgc gcatcctcgc gcgccgcgag cccggcgtgc cggtccgccg
     1501 tctcgatctc gaggccgcct ccgcgatgga gcggcccgag gtgaagctgc tcgtcgaggt
     1561 cctgcgcctc gacagctcca acccgcccgg aatcacgcgg cccgtgatcg acgtcttcgc
     1621 gcgggagctc gcgtgcgcgg gaatcccacc ggtcgtgacc ggccgcgacc cccagcgccc
     1681 gatcctcgtg gcccggctgc cgggccggcg ccgcggagag gcgctcgcgc ttctcgcgca
     1741 cgcggacgtc gtcccggcgg gcaacctctc ggactgggtg aagcccccgt tcgccgccga
     1801 acgtggagac aaggagtacg cctcgaacat catcgcgcgc ggtgctctcg acatgaaggc
     1861 gcaggcggtc gcgcacctcc ttgcgctcac ggcgctcgcg cggtcgggca tcgcgcccga
     1921 gcgcgacatc gtgttcgtca tggagtcggg ggaggagtcc tacgaccaga cgctcgggtt
     1981 cgggtggctg ctcgcgaatc gtcccgacct cgtggccggc gtcacggacg tcttcaacga
     2041 gggcggcgtc aacgaggtga acacgacgga gatcgagagg ttcggcgtcg aggtcatgca
     2101 gaaggcgatc ctctccgtgg acgtcctcgc ggccgagaaa ggcccgctcg aggacctcgc
     2161 ggcggtcgtg aaggcgcggg acgaagccga gcctctgaag gtggtgccgg cggtgcggga
     2221 cttcctgcgg ttcatcgcgc cctcccgatc ggacgtctgg gggcgccgga tgctcgacga
     2281 ccccgcgaag atcggaccgg ggacggaatt ctggaaggtc gtgccggagg tctaccgttc
     2341 gctcctgaag gacatggcct acacgggccg cattgcggag cggcccgagg ggggggtctc
     2401 gatgcgggtc gtccggacgc tcctcccggg ctcgtccgtg gcggcggccg aggcggagct
     2461 cgacggctgg ctcctcgcgc ggcacctcac gaagcgcatc gtcctcaaga cggacgactg
     2521 cgtcgcgact ccggccgaag gcccggcctg gcgcgccgtg acggaggttt tcggtctgga
     2581 tccggcccac gtgccggtcg ggccgtacat cctgtcgggc cagtacacgt cgagctcgtt
     2641 cctgagggcc agggggctgc gcgccttcgg cgtctcgccc ttcgcggtcg gctacttcga
     2701 cgccacgacg atccaccacc acagcgagcg catcgtggtc ccgttctacc tcgaggggct
     2761 cgagcggatg aagaggatcc tgttcgagtt cgcgaccgcg ccctgacagg ccggctgcta
     2821 cactttgggg ccggaggggc cgtgggacgc gtcgacttct actaccatcg ggtcggttga
     2881 acgagctgcg gacgcacccg tgaggtgctc gagaagtcga agtcggacgt gcgcgacgag
     2941 cgcccggccc gttcccgccc gctcacggac gcggacgcga aggccctgct gaaggaagtc
     3001 gacgaggtga cgctcgcgag gggcaaaacg gcacggaccc tcccggcgcg cgaggtctcc
     3061 ctcgacgacc tccgggggcc gacgggcaac ttccgcgcgc cgatgctgcg cgtcgggcgc
     3121 cgccttctcg tcggatggca cgaagaatcg ctccgggagc tgctggcacg gtcctcgcgt
     3181 tgatggttcg cccgcgccga tccggcggcg ggaaatgggt tcaggagaac ggatgaaaac
     3241 gactctcgtc acgtgtgcgg tcctgctcgc ggctctgcct gcggcggccc aggtccaggt
     3301 ccagacgcag caggtgggcc ccggcccggg catcgcggct ccgcagcagc cttccccgat
     3361 cccgtgcccc ggcatgggcg gcctcgaggt cggccagacg aggacgttcg cgttcgagcg
     3421 gatggcgaag ctcgagcccg gcaaggcgaa ggtcacgcgc gacgtgaagg tcgtccacaa
     3481 gcccgaggga cggccgtggg tcatcacggc cacgttcgac tcggatgccg cggacgcgaa
     3541 ggtcctcggc ctgtactact acgtcgaccc gccgtccggc atcggcgacg ccctgcgcga
     3601 gcgctacggc aagggaaccg cggtgccttc tgccgcggac cagacgtatt gggacgtcgg
     3661 ctcgtgcgac gtgcgcctca agtaccgcgc gcgcatgaac gagaagcagc ggccgatcga
     3721 ggagctctgg gtcgacccga tcccgaagcc ggccccctcc aaggccgcgc ccgcaaaaaa
     3781 gaagggctga agaaaagggg gagaagagga agacttcctc gaaggtgccc gtgcttggcg
     3841 ccgggccgac gcgtttcggg tggcgcttgt gccatcctcc gcgcggatga agagggtcct
     3901 caaggccgtc gccctcgcac ttgccctcgc gatcctggtc gccgccggcg ccctgctctt
     3961 cctcattcga agaaatctcc cttcttcttc ctccccgaaa attctcggcc tctctgcgaa
     4021 ggtctccgtc tcgttcgacg accgcggcgt cgcgacggtc acggccgcga ccgtccggga
     4081 cgcgctgcgc gcgcagggct ttctcacggc gcgcgagcgc ctcttccagc tcgagctgca
     4141 gcggcgcctc gcgcgcggcg cgctggccga ggtcttcggg gcgtcgcttc ttcccgcgga
     4201 ccgtctccac cgcacctacg ggttcgcccg tgtcgccgag gctgccgtgc cgctccttcc
     4261 cgagggggag cgcgccgacc tcgaggcgct cgcggacggg atcaacgcct tcctcgacgc
     4321 gcggcccggg cggctcggga tcgagttcac gctgctcggc ctctcgccgc gacgtttcac
     4381 cgcggccgac gccctcgcgg tccacctcct catgtgcgag gacctttcga cctcgtggcg
     4441 gcgcgatctc gcgctcgaga agctgtcggg cgccgcaccc tccgtccgcc ggttcgtggc
     4501 cgacacgacg tccccgtccg acgtcctcct cctgcccgac tccgctcctc tggtggcgcc
     4561 tccgctcccc gaccttggcg gcgtgccgct cgccggcctg ggtttcggaa cgttgaggga
     4621 agacgagccg cgcgggtcga acgcctgggc cgtggccggg atcctcgcga agagcgggaa
     4681 accgctcttc gcgaacgacc cccacctcgc cctgacgctt ccggggatct ggctcccgat
     4741 gcgcttcgtg atcgggggcc gcctcgtcga gggcgtcacg ctccccggcc tgcccggcgt
     4801 gatcctcggc cggaacgagc gcgtcgcctg gggcatgacg acgctccgga cggacgtgca
     4861 ggacctctac cgcgaaacga tcgtgggcaa gagggtgaag cgcggcgccg gctgggagga
     4921 cgtcagggag cgaaccgaga cgatcgccgt ccgcggagga aagcccgaga cgctcgtcgt
     4981 ccgcgagacg tcgcacggtc ccctcgtcca ggggcgcatg gccctgaagt ggctcgccct
     5041 cgacccgtcg ctcatgcgcg ttcccgagag cgccgtcatg acggcgaccg accgcgagtc
     5101 gctttctcgc gcgctggacg cgtttcccta tcccgcccac aacgtcgtgt gggcggaagc
     5161 ggccggctcg atcggctggc gcgccacggg cctcgtcccg atccgtcgaa cgggcacgga
     5221 cgggagcgtc ccgtacgacg gtgccgatcc ggaaaacgac tggaagggct acgtcccgcc
     5281 ttcggacatg ccgcgcgtcg tcaacccgcc cgagggcttc gtcgtgaccg cgaaccagcg
     5341 cgacatcggg acgcggttcc cgcagaccgt cacgacggac tggccgtcca cggaacgcgc
     5401 acgccgcatc gcggaccttc tcggcgaggc ggcgcgcggg aagaggctgc tcgaccgcgc
     5461 cgcgatggag gccctccagc tcgacgacgt gtcccccgtt ctcgcggcga cgctctctgc
     5521 gctcgcaccc gagctgccgg agccctggaa cgcccgcttc gcgggctggg acggccgcgc
     5581 ggacgcggac tcgacgctct tcctcgttgc gcgctccacg cgccgaaagc tcgtcgagcg
     5641 cgtccttgcg gcgtggcgcg tgccgcccga ggtcggtctt ccggacgcgc gcctcctcga
     5701 gctcgcgcgc gcggacgacg cggcgttccg gcgggcgctt ctcggcccga agcgcgacct
     5761 cgtccgggcc gccttcgaag cgtcccgtgc cgacctgacc gcgcgcttcg gccgcggcga
     5821 ggcgcgctgg acgtggggcg cgtcgaaccg cctcgccgta aagcacccgc tcgggcgtct
     5881 gccgggcctc ggcggactct tcgacccgcc ttcgttcccg cagtcgggtg cgggcggcac
     5941 gcctcgcgtc gcgacgccga cgtacggcca gtccatgcga ttcgtcgtcg actggggagc
     6001 cccggacgag gcgacgctcg tgatcccgtt cggagtctcc ggccacctcg ggtctgcgca
     6061 ccgtaccgac cagctggccg cgtggcggga gggggatccc gggggcgcgc ggacgaggct
     6121 ggcccggccg gccgtgacga gcctggagat cacgccgtga ccggcgccgg cgctcttctc
     6181 gccgccgtcc tgtccgttgc ctcggcgccg aggacgattc cgcagggagg cgtcgcggag
     6241 atccgcatcg cgagcgatgc gccccttcca gagctcgtgc tcgtcgacgg cgaccgacgc
     6301 gtcccgctcg agcgggatgc gtccggccgg aaattcagcg gcctcgtcgg cgtcgatctc
     6361 gacgcgaaga ccgggctgca cgacttcgtg gtggaggctc cggacggggc ggcgcgcctc
     6421 gtccttgcgc tgaaggtcct gcgcggctct ttccgcatcc agaagctcac ggtcgacccg
     6481 aagttcgtcg aggtcccccc cgaggagaag gagcgggtca aggcggacgg ggagcgtgtg
     6541 aacgcggctt accggagcgc ggaagcggag cggcgctgga cggccttcgt ccgccccgtc
     6601 gcctatgacg gaaccaattt cggctcgcgg cgggtctaca acggcaagac gcagagcttt
     6661 cacgcgggcc tcgacatgcc ggcgcccgcc ggcacgccgg tcgcggcggc cgcggacggg
     6721 cgcgtcgcgc tcgccggcga cctctacttc tccggcggaa ccgtcctcct cgaccacggc
     6781 ggcggcctct tcacgcaata catgcacctc tcgcgcgtgg acgtgaagga cggcgacgtc
     6841 gtgaagaagg gtgcgatcgt cggcctcgcc ggggcgacgg gccgcgtcac gggcccgcac
     6901 ctgcactggg gggcccgctg gaacggggcg cgcgtgaacc cgctggcgct cctcgcgctc
     6961 ccggcgcgcc cggcgccggg tctacactga ggcatgagcg tcctcatcac gggccggctc
     7021 gtgtctccga cggcgaccga gctcgaccac ggtccctcgc acaagaggct cccgacgcag
     7081 gctccgaagg acaacggcgg cgacggcagc agcttctcgc cgaccgatct ctgcgcggcc
     7141 tcgctcggtt cgtgcgccgc gacgacgctg agcctctacg cgacccgcgc cgggatcccg
     7201 ctgaccggcg tcacgttttc cgtcgagaag cacatgacgt ccgagccccc gcgccggatc
     7261 gcgaggctcg tcctggagct gacgatcgac tcgccgtgcg gcgacgaaga cttcgggaag
     7321 ctcgtcaacg ccgcgaagtc ctgccccgtg cggcgctcgc tccacccgga cgtcgtcgtc
     7381 gacgagacgt acgttcgcgc ctgacgcgtc agcccgcggg cgtccaggtt ccggtggggc
     7441 tcaggggaat ccgttcggcc tcgacctcga agccggcctc ggcccaggcg tccagaccgc
     7501 cctcgagcgg gcggacgagc cggagcccgc ggtccatcag gaggcgtgcc acacgggccg
     7561 ccgaggcttc gttgggtcac gtgcagtaga ggacgatctc ccggtccgcc gggaggagcg
     7621 cgacgtgcgc gtcgaggtcc tccaggtcga tcgtgaccgc cccggggatc cggcgcggat
     7681 cccgcaggcg cgcgttcctc gaacggacgt ccacgacgac gggcgcctca cccgcgtcca
     7741 tcagcgaccg gaggtcggcg acgcggatgc gcgccatgcg gagaaagcgg taaaagcgct
     7801 gccgctggtg ccacttgaag aggatgtaga gcaggagtcc gcccgccagg accacgaacg
     7861 cccagccccc catgttcgag aggagtgcga gaacgcggtc cacggcgccg tggaacacga
     7921 cgccgaggag gagcgcggag ccggcccaga gaagggttcc gcccgacgtg aagaggagga
     7981 agcgggggta gccggtgccg gatgcgcccg cgaggggcgg cgccacggtg gagtagcccg
     8041 ggacgaactt cgcgaagaga agggaggcga ggccgtactt ctcgaacatg ccttccgtct
     8101 gacgcacgca ggtgtccggc gagagcgaga cgcggcagag cgtcttgagg agactctgcc
     8161 cgtaccggcg gccgaggagg aaccagagcg agtccgccgc gaccgttgcg acgaacgccg
     8221 cgaggaggag gtgcgcgaac gagagttttc cgtcggcggc gagcgcgccc gcgacgacga
     8281 gcgtggggac ggcgggcacc ggcaggccga gctgctcgag gaggacgttt ccgaaaacga
     8341 gactcgttcc gtgcttgagg acgagggcga agagatcctt cacgccgctc tcagctcacg
     8401 cccgtcgacg acgaggagac ggagcccggg tccacgccga ggcgcgcgag ggcgcgctcc
     8461 cagagcgtct cgacccttct cgtgaaggcg aggtccggct cccacgggag gacgagccac
     8521 gccccctcct ccatctcgcc ctcgagctgc tcggcgtccc agccggcgta gccgagcacg
     8581 aggaggccct ggggcggccc atcgcccggc ggcttgccga agagcttcgg gaggatctcc
     8641 gcggacgccg agacccagag gccgggggcc atttcgttgc agtccacgcc gtcgacctcc
     8701 tcgcggaaga gcgcgacgag tccgttcgtg aggacgggac ctccgatgtg gagcggcgtg
     8761 gcggggtgga gcgtcgcgtc gagaggcggg ggcaggagcg tcaccgccgg ctgcccgagg
     8821 aggcggttca cgacccagcc gagcgccccg tccttcgtgt ggtggcccat caggacgacg
     8881 gtcttcgaga agttcgggtc cgcgaggccc ggccctgcca gcaggaggta cggcgccgtc
     8941 agggtctcga ccacgggggg aatttaccgc accggcgccc gggcccgcgt agcatgtccc
     9001 gcatgaccgt gcctggcagc ggcctcctgc tccttgcggt cgcgagcgtc gtcgtcctcc
     9061 tcgtcctcct cgaggcctgg cggcagaggc ggatctacgg gaagccgtcc gggcggccga
     9121 acctcgccgg agccgggatg ctcgagctcc agaagcacct ccaggccgac cggcgtgtcg
     9181 agaagctcgt cgagatccag cgggacgagt ccgccgagac cgagcaggac gaggcgggcg
     9241 gcccgcgcca gccgcccgcg cgtccgcccc gctagcgggc cggcgcgccc gcctcccagg
     9301 ccgccttcgc gatcgaggcg atcgccttct cgcgcgcctc cgcgccggca ccgccgcgcg
     9361 tgaacacggc gaggaccgcg tgcgagccgt cgcgcagcgt caggattccg acgtcgttcg
     9421 acgagagccg catcgtcccg gtcttgtcga agacctccgc gcccttcggg aggcccgccc
     9481 ggaagcgccg gccgctcgtc gcgcaccgcc gcatcgagtc caggatccgg tcggtggagt
     9541 ccttcgagag gagctcgccg cggaagaggc ggacgaggag accggtcatg gcgtccgggc
     9601 tcgtcccgtt ctgcgcgccg cgttcgatcg cgtcgaggtc catcctcatc gccgcgccga
     9661 ggcccctctc gtccagcgac accttcacgc ccgtgacgcc tttggccacg agcgccttct
     9721 cgacggccgc cggccctccc agctgcgcga cgaggacgtc ggccgcggtg ttgtcgctgt
     9781 cgacgagcat gacgtcgacc atgcgcgcga tcgtgacgcg catgccgggc ttccactcgt
     9841 cgtagatcgg cccgacttcc atgcggtcct tctcgcggat ctcgacctcg ccgtcgaggc
     9901 ggagcttctc cctttccacg gcgtcgagga ccgcgatggc gacgggcagc ttgaagacgc
     9961 tcgccatctg gaacgtctcg gtgccgcgcc aggaggccgc gcggccgctc tcgaggtgcc
    10021 gatacgacac gcccgcgacg aggccgcccg cctcggctgc gcgcgcggcg gcgacggcgg
    10081 gatggtcggg cacggggtcg ggagcgggag cggcggacgc gaaagcaaag aggacgaccg
    10141 ggagaacgag gatcaaaggg aacctcctaa gaagattttc ttagaagggg gaagaacaag
    10201 aggcgtgcca ccgggcagag gctgccctgc gccgcgcgcc ggcccttcct ttctccgttt
    10261 ctaagaaatc cggaaaacaa cgcagcaaac cggtccggcc gctcccgccc tactcctcca
    10321 ggtacgtgta gtgcgtaagg ctgtgctcga agtccttcgc gatggccttc gcctcgggct
    10381 ccttgacctt cttgcgctcg gtggcccggc gcagctggcg ccagatgccg tcgaggagct
    10441 cgtccttgtc gtagttcatg tacgaaagga ccctctcgca ggactcgccg ggcaggacgt
    10501 cctggatgcg cgtgcgtcca tcgtcgtcca cgctcgcgag cacctcgtgg acctcgccga
    10561 acaggttgtg gaggtcgccg aggacgtcct ggtacgcccc gacgaggcag aacccgatgt
    10621 aatacggcgt gcccttgtgg aaggcgtgta gcgggagcgc ttccttgatg tcgcgcaggt
    10681 cgatgaactt ctcgatcttg ccgtccgagt cgcacgtgat gtccgcgagc gtcccgtgct
    10741 cggacggcct ctcgcgcagg cggtggatcg ggaggatcgg gaagagctgg tcgatcgccc
    10801 agacgtcggg cagcgactgg aagaccgaga agttcatgac gtacttgtcg gcgagcatga
    10861 cctcgagctg ctcgaactcc tccgggatgt ccttcagcgt gtggaggtac cggcggatgc
    10921 ggtcgcagat gttccagtag aggatctcgc ccttgctccg gtcctcgagc gacacgtagc
    10981 cgaggttgaa gagcgtgaag agctcctcct tgtactggag ggcgtcgtgg tagctctcgc
    11041 ggacgttctt cgccgtgaca ccctgccaga tgtcgtagag ctcgcgcacg acgtggttct
    11101 cgtcgccgtc gagcttcacg gagcggccct gctcgatgcg gtccgcgacg tcgaggacgt
    11161 tcgtgacgag gacggagtgg tacgccgtca gcgcgcggcc cgactccgtg acgagcgtcg
    11221 ggacggggac cttctcctcg tcgcagatcg acttgatcgt gtagacgatg tcgttcgcgt
    11281 actcctggac cgtgtagttc atcgaggagt cgaacgccgt cttgcttccg tcgtagtcga
    11341 cgccgaggcc tccgccgagg ttcaggtact ggatctcgac gccggtcgcg tggagcttcg
    11401 cgtagacgcg ccccgcctcc ttgatcgcct gcttgatctt ccggatgtcc gtgatctgcg
    11461 agccgatgtg gaaatggagc atgacgagcg agtcgagcat cttgcgctgc ttgaggatct
    11521 cgacggcctc gagcatctcg gtcgtcgtga ggccgaactt tgcggcctcg ccgccggact
    11581 tcgcccactt gcccgagccg cgcgcgtaga gcttgctgcg catgccgatc aggggcctca
    11641 cgccgcgctt cttggcgacg tcgaggatct tttcgagctc ggagaccttc tcgaggatca
    11701 ggacgaccgt cttgcccatc cggatgccgt tgagggcgag gcggatgaac gcgtcgtcct
    11761 tgtagccgtt cgtcgtgatg aggcagtccg agccgaggtc ctgcgagagc gccgcgatga
    11821 gctccggctt gctcccggcc tccagcccgt agccgtactt gcggccggac tggatgatct
    11881 cgcggacgac gaccttcttc tggttgacct tgatggggaa cacgcctcgg aagacgccgt
    11941 cgtagtcgta ctccttgatc gcgtggagga aggcctcgtt cagctccttg acggcctggt
    12001 cgaggatctg cgggaagcgg atcacgacgg gcgtcgagat gccgcgcagt gcgacgtcgt
    12061 ccacgacacc cttgaggtcg acgaagcggt ggttctcctt cgtcgggtgg acgaggaggt
    12121 tgccgtcgtc ggagacggag aagaagcctt ttccccagtt ctcgatgccg tagagcttct
    12181 cggcgtcggc gaaccgctcg ggggctcggg cgcgcgcggg cttcttcgag gcgcgcgcga
    12241 gtgcagtggg tgtcatcggc gcgaatccta aacgaacgcg cgcggagggg cgggaaattc
    12301 tctaggatgg gcgcgtggcc gcccccctcg acctgacgct ggcccgccgc tggggcctgc
    12361 ccgccggggc caccgcctgg cgcatcacca ccgacctgca ggccgacatt gtcgccttct
    12421 cccagaaccg cggcctgttc gccggcctgt ccctgcaggg cagcgtgctg aaccagcgca
    12481 gcgactggaa ccgatcatac tatgggcggg agatgtcccc gcagcagatc gt
//
LOCUS       DAOMFY010000098        12347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1585_length_12347_cov_8.123576, whole genome shotgun sequence.
ACCESSION   DAOMFY010000098 DAOMFY010000000
VERSION     DAOMFY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1585_length_12347_cov_8.123576"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLB01_09735"
     CDS             <1..645
                     /locus_tag="PLB01_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77622.1"
                     /translation="ERAAAERALAAAARAGGDTARDVEEDLETFTALLARATVFRDAV
                     DLARESLGGAAASVYSDFRRGLNEASRAILATWRTPYEALEFSDDLTLSARVKGGRIA
                     TKTEIEAGLSTGAREQLHLTARLAALRYLGTGAAGVPLLLDDPLVGADDERFVAVMRF
                     LATEVLGERPVLVVSCHAWRHERLLAALGDAAPRLARVSLAPFSSRPAGVAATD"
     gene            771..2402
                     /gene="secD"
                     /locus_tag="PLB01_09740"
     CDS             771..2402
                     /gene="secD"
                     /locus_tag="PLB01_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQQ77623.1"
                     /translation="MNKPTGWRVGVTLAVLLGSIGAYWWRGHEALKKSPTAPPNPSMS
                     EVLRNGIKLGLDLKGGIHLVLQVKTADALRVEAQDTLEHLKDEGTKRGVPVTPAGPAD
                     ATGFTLKLDEKTDQAKLKDLVKSILPPNSWSVDERGREWKIGFKDVEKNAIEDQATRQ
                     AVETIRNRVDALGVAEPVIQRQGTDRIVVEIPGVDDPSRVKEIIGTTGLLELTLVDDR
                     GGPYPTAEGAKAAYGGVIPEDLRLVEGFSEDADARTRTPVFYVLKRAAAVTGRDMKNA
                     RVDRDKFNQPAVMFFLNAPGAVKFAKVTGENINKHLAIVLDNRVMSAPNINSQIKESG
                     IIEGNFTAEKADALALVLRSGALPAEMIVLEERTVGPSLGLDSIKKGVLASIVGMICV
                     FVAVVLYYRLAGVNAVVALVLNALILLGAMAYINATLTLPGIAGFILTIGMAVDSNVL
                     IFERIREEMDNGKAPKNAIDLGFKRAWGTIVDTHVTTISAALFLFQFGTGPVKGFAVT
                     LVIGLLASVFTSVFVSHLLFDLIYGPKERVETLSI"
     gene            2416..3639
                     /gene="secF"
                     /locus_tag="PLB01_09745"
     CDS             2416..3639
                     /gene="secF"
                     /locus_tag="PLB01_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQQ77624.1"
                     /translation="MRLFHNTNIDFLKIKWICISLSILSIVVGTISLVAKGGPNLGID
                     FTGGAQIVYAFSKTPDEDQIRKIVEAANVKVVSVQRYDKAERNQVLLRVPMEKKEGRD
                     VSKEVTAALTAALHPAGASADAFDLNLNGADLLLKKLIADDPEHAAQRPSVDPKVEYG
                     RIAQALIAARSERGLFASVDDAAKTPGVSPTVAEWLKARTVAGPFTLLSAENVGPQVG
                     KDLREKGMWAILFSWGAMLLYIAVRFRSASFGTAAVVALIHDTWTTLGLCSILNLEIS
                     LTVVAAFLTLVGYSVNDTVVVFDRIRENREKNRRAPLAELVNHSINQTLSRTVLTSLL
                     TFLVVVVLFFLGGEVLRGFSFVMIIGIIVGTYSSIFVAAPLVIVWEEWRMKRAAAKSA
                     GAPAPAKPGKVAKAR"
     gene            complement(3734..4975)
                     /locus_tag="PLB01_09750"
     CDS             complement(3734..4975)
                     /locus_tag="PLB01_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQQ77625.1"
                     /translation="MSGGRARSPWLSSTALIFYALVLGVVLGHFLPAETYPWAYESFR
                     FLSKAFINLIKMLVVPLIFSTIVLGIGKTGDVKTVGRMGVKALIYFEIATTIALVIGL
                     LFANLVKPGLDLKLPATGEILAKPKTAVETLLHLFPSNVIDAMARQDILQIVIFATLL
                     GVAAAHVGHERAKPFVDFFESAVAVLFKLTDYVMALTPLGVFGAMAGAVSHHGITVLG
                     SYAKLVGSLYAALLVFALVVLVPALKICRVSIRRFFAKIREPFLIAFSTASSEAALPR
                     ALERIVEFGVPKGVAGFVLPAGYSFNLDGSTLYIVLATMTIAQAAGRHLTIAEQITMC
                     LVFMLTSKGVAAVPRASLVIIAAGCASFNLPGEAGVAMILAVDELMDMARTSINLAGN
                     CVAAVVVAKWEGVLGPEAKSV"
     gene            complement(4972..5622)
                     /locus_tag="PLB01_09755"
     CDS             complement(4972..5622)
                     /locus_tag="PLB01_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HQQ77626.1"
                     /translation="MDLLKQLIDVFLHIDKHLAEVTRDYGAWTNAILFAIVFCETGLV
                     VTPFLPGDSLLFTAGALASLGTLNVWVLFLLLSVAAILGDTVNYWIGKKIGPRAFDGT
                     VKFLKQEHLRKTEAFYEKHGKKTIILARFVPIVRTFAPFVAGVGSMAYGTFIAYNVIG
                     GVAWVAICVFSGYFFGNIPIVKKNFSLVVLAIIGISVLPLAWEWWKARQPRGGTAA"
     gene            5676..7859
                     /locus_tag="PLB01_09760"
     CDS             5676..7859
                     /locus_tag="PLB01_09760"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQQ77627.1"
                     /translation="MLRFEDILEKVESYHPHVDEDLLRRAYVVSAHEHRNQLRSSGEP
                     YLVHPLNVAMILAEMKLDEASIAAGLLHDVLEDSSMTKERVGELFGPDVAHLVDGVTK
                     IGKYAFTSKEAQQAETFRKMLLAMTDDLRVILVKLADRLHNMRTLEHLPEAKQRTIAA
                     ETMEIYAPLANRLGMGKMKGELEDLSFRFLYPEEFAALQTAVDERMKASAANVEKFRQ
                     ELIAKTQAAGIEAEITGRVKRYWSVRQKLIRQAIPLEQLYDVLAFRILVSSIPDCYTV
                     LGIVHQAWRPVPGRIKDYIAMPKRNFYQSLHTTLVPEGAVPFEVQIRTREMDLIAENG
                     IAAHWKYKEGESSRAQEAHVGLLRQIFETTRDVSDPREFLSSLRIDLYPDDVYIFSPK
                     GAVFAFPRGATPVDFAYRVHTDVGHHTTGARVNGRLVPLKTPLANGDIVEILTSPSSQ
                     PSRDWLNFVLTARAKNKIRAFIHTAEKEKSVEIGRRLLDRELKKWKKSLPKLLEAKAF
                     EPFLGDFGVSKVDDLIAEVGFGKLAPRAVVAKLLPEAGGEAQAPQPSRVAAIGNAVRR
                     VLPFGSAPGIRVKGENDLLATLAKCCQPVPGEEIVGYVSRGRGVSVHSVGCPNVRSLL
                     FDPSREIAVQWETAKDQSVTVDLEIHTEDRQGMLARITQLLSAANSNIRSIEARTSGD
                     GRATIDASVTTQDRRHLERLLVGLRALPGVTGVRRKLHTSDAEAG"
     gene            8143..9198
                     /gene="pilM"
                     /locus_tag="PLB01_09765"
     CDS             8143..9198
                     /gene="pilM"
                     /locus_tag="PLB01_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HQQ77628.1"
                     /translation="MLFRKAKNLVGLDIGSSAVKLVELKEGKEGTFHLVKAGLETLSP
                     EAIVDGAIMDSSLVVETITRLLASTGVKNPNVAVSVSGHSVIVKKIMLPTMPEEELSE
                     SIRWEAEQYVPFDINDVYLDYVVLDPGAVGETMGVLLVAAKKEKVDDFKNVVTQAGRT
                     PSLVDVDAFALQNCYEVNYGADPGRVVALVNIGASVMNVNILAQGQTVFWRDITFGGN
                     QFTDALQKAYSLNFEQAESLKKGEPVAGQTAQSIQPVLTGVSEDAAAELQKTLDFFQA
                     TSGADRVDSIVVSGGGSKAAHFEDVLKDKFGLPVEVMNPFRSIFTDDRAVDAAWLSEA
                     SPALAIAVGLGVRKIGE"
     gene            9203..9874
                     /locus_tag="PLB01_09770"
     CDS             9203..9874
                     /locus_tag="PLB01_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQQ77629.1"
                     /translation="MIRINLLAEARPQRKKKGVSALGAGGRLNTLLLGGAIGLAVLVG
                     VIHYWVLRSQIKAQDEKIRIAQVEVTRLESVLKEVKDYEAKKARVQRKVDLINQLKQN
                     QRGPVRLMDEVSRALPDLVWIERMEYHGNAISLEGKAFNPPAVANFIANLRLVPAFQE
                     PKLADLSACSGASGSTLYCYKMSFTFSNLDRTQPETAAPAPAKPAEPPKKAERAAGGA
                     AAQGM"
     gene            9888..10553
                     /gene="pilO"
                     /locus_tag="PLB01_09775"
     CDS             9888..10553
                     /gene="pilO"
                     /locus_tag="PLB01_09775"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HQQ77630.1"
                     /translation="MASLDLKQLEGKPWYYGLGIGLAIAIALYAVAYYQIPNFAEMKQ
                     TLENKKNELDALQQKITQGRAAERRLPQLREEVRRTELDLQRLLAILPTARNAEELIK
                     KVQALAGQGELFTKKWSPKEYINRDFYAEWPIDLQVDGSYHNLALFFEKVARFSRIIN
                     IEDLVMTGYPDARGGRTLGANFTAKTFIYLGDTEPATPAGPKAAPKAGPKPAGGAKKD
                     MEG"
     gene            10550..11041
                     /locus_tag="PLB01_09780"
     CDS             10550..11041
                     /locus_tag="PLB01_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HQQ77631.1"
                     /translation="MSARHATLLAAAAFAVFAVPAAGPAAAQQGTAPPPPAETPKITD
                     QDFTPSVYTYEVGGRRDPFRSLLVRNPSEKERVRPPGLAGVMVDELELQGTIKVKQGW
                     VAMMRGPDNKSHLIRKGTTLFDGEVTDVSATEVTFRQNVNDPTNPKPFREVVKALALQ
                     KKP"
     gene            11128..>12347
                     /locus_tag="PLB01_09785"
     CDS             11128..>12347
                     /locus_tag="PLB01_09785"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HQQ77632.1"
                     /translation="MRNPCVLAARVAALALLVPVLSGCASSGDNVAAGSSGTTAPAPV
                     VLTGATLLADGDSPRLLLTGNGPLAPTLYNREGSTKVVVDVANAVAAPGLEPPRADGT
                     LLSRLEMKSFVEMGKPHIQFELTARVPLDPAISSESGSPAMAVSLVKATSVMTASAAA
                     PAGPAPAAASAPEIPVVKVEAEPAVKVEEVPAAATTSTTAIAVASDAAPVPVRHAASG
                     PRATRLSGVAVAQSGGGLVVTLSGNGSFAYETFALANPPRYVVDLPGVLLATPKKLQD
                     VRHPAVTRVRASQFKGGSEPVTRVVFDLANPVEPAARSASAGLAFAFGTGSSAVASAP
                     AVREAPAPVVKEAAAPLVKVEAPAPALNTSAPAPAPSVAEAQTAPAPAPGKVTAAPEK
                     VAVVAAPAASSYER"
BASE COUNT         2088 a         4315 c         4133 g         1811 t
ORIGIN      
        1 gagcgggcgg cggccgagag ggcgctcgcg gccgccgcgc gcgcgggagg ggacacggcg
       61 cgggacgtcg aggaggatct cgagacgttc acggcgctcc tcgcgcgcgc gaccgtgttt
      121 cgcgacgccg tcgacctcgc gcgtgaatcc ctcggcgggg ccgcggcgtc cgtctactcg
      181 gacttccggc gcggcctcaa cgaggcctcg cgcgcgatcc tcgccacgtg gcggaccccc
      241 tacgaggcgc tcgaattctc ggacgacctc acgctctcgg cgcgcgtgaa gggcgggcgc
      301 atcgcgacga agaccgagat cgaggcgggc ctctccaccg gcgcccggga gcagctgcac
      361 ctcacggcgc ggctcgccgc gctgcgctac ctggggaccg gggcggccgg cgtcccgctc
      421 ctcctcgacg atccgctcgt cggcgccgac gacgagcgct tcgtcgccgt catgaggttc
      481 ctcgcgaccg aggtcctggg ggagaggccg gtcctcgtcg tgtcgtgcca cgcgtggcgg
      541 cacgagcgtc tcctcgcggc gctcggagat gcggcgccgc ggctggctcg cgtgtccctc
      601 gcgccgttct cgtcccggcc ggcgggcgtc gccgcgacgg actgaccgcc cccggggttc
      661 tgctagactc cgccggttcg cccggagaag ccgggtgtat cccgcgggtt tccccgtcgc
      721 tccggtggcc tcggccgcct cggccgggcc cgctcccgga ggttgcgttc gtgaacaagc
      781 ccaccggatg gcgcgtcggc gtgacgttgg ccgtcctcct cggttcgatc ggcgcctact
      841 ggtggcgcgg ccacgaggcg ctcaagaagt cgcccacggc cccgccgaac ccctcgatgt
      901 ccgaggtcct tcgaaacggc atcaagctcg gcctcgacct caagggcggg atccacctcg
      961 tcctccaggt gaagacggcc gacgcgctgc gcgtcgaggc gcaggacacg ctcgagcacc
     1021 tcaaggacga gggcacgaag cgcggcgtcc cggtgacccc ggcgggtccc gcggacgcca
     1081 cgggcttcac gctgaagctc gacgagaaga ccgaccaggc caagctcaag gacctcgtca
     1141 agtcgatcct cccccccaac tcgtggtcgg tcgacgagcg cggccgggag tggaagatcg
     1201 ggttcaagga cgtcgagaag aacgcgatcg aggaccaggc cacgcgccag gccgtcgaga
     1261 cgatccgcaa ccgcgtcgac gcgctcggcg tggccgagcc cgtcatccag cgccagggaa
     1321 ccgaccgcat cgtggtcgag atccccggcg tcgacgaccc gagccgcgtg aaggagatca
     1381 tcgggacgac cggcctcctc gagctcacgc tcgtcgacga ccgcggcggc ccgtacccga
     1441 ccgcggaggg cgcgaaggcg gcctacggcg gcgtgatccc ggaggacctc cgtctcgtcg
     1501 aagggttcag cgaggacgcc gacgcgcgga cgcgcacgcc ggtcttctac gtcctcaagc
     1561 gcgcggccgc cgtcacggga cgcgacatga agaacgcccg cgtggaccgc gacaagttca
     1621 accagcccgc cgtcatgttc ttcctgaacg cgccgggcgc cgtgaagttc gcgaaggtca
     1681 ccggcgagaa catcaacaag cacctcgcga tcgtcctcga caaccgcgtg atgtccgctc
     1741 cgaacatcaa cagccagatc aaggagagcg gcatcatcga aggcaacttc acggccgaga
     1801 aggcggacgc gctcgcgctc gtcctgcgct cgggtgcgct gcccgccgag atgatcgtcc
     1861 tcgaggagcg cacggtcggg ccgtcgctcg gcctcgactc gatcaagaag ggcgttctcg
     1921 cctcgatcgt cggcatgatc tgcgtcttcg tcgcggtcgt cctctactac aggctcgcgg
     1981 gcgtcaacgc ggtcgtcgcg ctcgtcctga acgcactcat cctcctcggc gcgatggcgt
     2041 acatcaacgc gacgctcacg ctgcccggca tcgcgggatt catcctcacg atcggcatgg
     2101 ccgtcgactc gaacgtcctc atcttcgagc gcatccgcga ggagatggac aacggcaagg
     2161 ctcccaagaa cgcgatcgac ctcggcttca agcgcgcctg ggggacgatc gtcgacacgc
     2221 acgtgacgac gatctcggcc gccctcttcc tcttccagtt cggtacgggc cccgtcaagg
     2281 gcttcgccgt cacgctcgtc atcggcctcc tggcctccgt cttcacgtcg gtcttcgtgt
     2341 cgcacctcct gttcgacctg atttacgggc cgaaggagcg cgtcgagacc ctgtccatct
     2401 gaatcggaga atcgcgtgcg cctcttccac aacacgaaca tcgacttcct gaagatcaag
     2461 tggatctgca tctcgctgtc gatcctctcg atcgtcgtcg ggacgatctc cctcgtcgcc
     2521 aagggcggcc cgaacctcgg catcgacttc acgggcggcg cgcagatcgt ctacgccttc
     2581 tcgaagacgc ccgacgagga ccagatccgc aagatcgtcg aagccgcgaa cgtcaaggtc
     2641 gtctccgtcc agagatacga caaggccgag cgcaaccagg tgctcctccg cgtcccgatg
     2701 gagaagaagg aaggccgcga cgtctcgaag gaagtgacgg cggccctcac ggcggcgctc
     2761 cacccggccg gcgcgtccgc ggatgccttc gacctcaatc tcaacggagc cgacctgctc
     2821 ctgaagaaac tcatcgccga cgacccggag cacgccgcgc agcgtccgag cgtcgacccg
     2881 aaggtcgagt acggccgcat cgcgcaggcg ctcatcgcgg cgcggtccga gaggggcctc
     2941 ttcgcgtccg tggacgacgc cgccaagacg ccgggcgtct cgccgaccgt ggcggagtgg
     3001 ctcaaggccc ggacggtcgc cggtccgttc accctgctgt cggccgagaa cgtcgggccg
     3061 caggtcggaa aggaccttcg cgagaagggc atgtgggcga tcctcttctc gtggggcgcg
     3121 atgctcctct acatcgcggt ccggttccgc tccgcctcgt tcggcacggc ggcggtcgtc
     3181 gcgctcatcc acgacacgtg gacgacgctc ggcctctgct cgatcctgaa cctcgagatc
     3241 tcgctcacgg tcgtcgccgc cttcctgacc ctcgtcggct actcggtcaa cgacaccgtc
     3301 gtcgtgttcg accggattcg cgagaaccgc gagaagaacc ggcgcgcgcc gctcgccgag
     3361 ctcgtgaacc actcgatcaa ccagacgctg tcgcggacgg tcctgacgtc gctcctcacg
     3421 ttcctcgtcg tcgtcgtcct cttcttcctc ggcggcgagg tcctgcgcgg gttctcgttc
     3481 gtcatgatca tcgggatcat cgtcggaacg tactcgtcga tcttcgtcgc ggctccgctc
     3541 gtgatcgtct gggaggagtg gaggatgaag cgcgcggcgg cgaagtccgc gggcgccccc
     3601 gctccggcga agcccggcaa ggtggccaag gccaggtaaa gagggcgcag aaaggtgagc
     3661 cggcgagcgc aagcgagcgg gcgagacttt cgagcggaag gggtgcggga aacccgcctg
     3721 acgggtttcc cgcctacacg ctcttcgcct ccgggccgag gacgccttcc catttcgcga
     3781 cgacgacggc cgcgacgcag ttgcccgcga ggttgatcga cgtgcgggcc atgtccatga
     3841 gctcgtcgac ggcgaggatc atcgcgacgc ccgcctcgcc cggcaggttg aaactcgcgc
     3901 agccggccgc gatgatcacg agcgaggcgc gcggcacggc ggcgaccccc ttggacgtga
     3961 gcatgaacac gaggcacatc gtgatctgct cggcgatcgt gaggtgccgc ccggcggcct
     4021 gcgcgatcgt catcgtcgcg aggacgatgt agagcgtcga gccgtcgagg ttgaagctgt
     4081 agcccgcggg caggacgaag cccgcgaccc ccttcgggac gccgaactcg acgatccgct
     4141 cgagcgcgcg ggggagcgcg gcctcggaag acgccgtcga gaacgcgatc aggaacggct
     4201 cgcggatctt cgcgaaaaac cgccggatcg agacgcggca gattttcaac gccgggacga
     4261 ggaccacgag cgcgaagacg aggagggcgg cgtagagcga accgacgagc ttcgcgtagc
     4321 tcccgaggac ggtgatcccg tggtgggaga cggcgcccgc catcgcgccg aagacgccga
     4381 gcggcgtcag cgccatgacg tagtccgtga gcttgaagag caccgcgacc gccgattcga
     4441 agaagtcgac gaagggcttc gcgcgctcgt ggccgacgtg ggccgccgcg acgccgagaa
     4501 gcgtcgcgaa gatgacgatc tgcaggatgt cctgtctcgc catcgcgtcg atcacgttgg
     4561 aggggaagag gtggaggagc gtctcgacgg ccgtcttcgg ctttgcgagg atctcgcccg
     4621 tggccgggag cttgagatcg aggccgggct tgacgaggtt cgcgaacaag aggccgatca
     4681 cgagcgcgat cgtcgtcgcg atctcgaagt agatgagagc cttgacgccc atgcggccga
     4741 cggtcttcac gtcgcccgtc ttcccgatcc cgaggacgat cgtcgagaag atcagaggga
     4801 cgacgagcat cttgatgagg ttgatgaagg ccttcgagag gaaccggaac gactcgtagg
     4861 cccacgggta cgtctcggcc gggaggaagt ggccgaggac gaccccgagg acgagcgcgt
     4921 agaagatgag cgccgtcgag gagagccagg ggctccgcgc gcggccgccg ctcacgccgc
     4981 ggtgcctccg cggggctgac gcgccttcca ccattcccac gcgagcggca ggaccgagat
     5041 cccgatgatc gcgaggacga cgagcgagaa gttcttcttc acgatcggga tgtttccgaa
     5101 gaagtatccc gagaagacgc agatcgcgac ccaggcgacg ccgccgatca cgttgtaggc
     5161 gatgaacgtc ccgtaggcca tcgagccgac gcctgcgacg aagggcgcga acgtccgcac
     5221 gatcgggacg aaccgcgcga ggatgatcgt cttcttgccg tgcttctcgt agaaagcctc
     5281 ggtcttcctc aggtgctcct gcttgaggaa cttcacggtc ccgtcgaagg cgcgcgggcc
     5341 gatcttcttc ccgatccaat agttgacggt gtcgccgagg atcgcggcga cggacaggag
     5401 gaggaagagg acccagacgt tgagcgtgcc gagcgacgcg agcgcgccgg ccgtgaagag
     5461 gagcgagtct ccgggcagga acggcgtcac gacgagcccc gtctcgcaga agacgatcgc
     5521 gaagaggatc gcgttcgtcc aggccccgta gtcccgcgtg acctcggcga gatgcttgtc
     5581 gatgtgcagg aagacgtcga tcagctgctt caacagatcc atttcggccc tcgttgggag
     5641 cggaggatag ccggaaagga cgataatggg cggccgtgct gcgcttcgag gacatcctcg
     5701 agaaggtcga gtcctaccac ccccacgtgg acgaggacct cctgaggcgc gcctacgtcg
     5761 tctccgcaca cgagcaccgg aaccagctgc gctcctcggg cgagccctac ctcgtccatc
     5821 ccctcaacgt cgcgatgatc ctcgcggaga tgaagctcga cgaggcgtcg atcgcggcgg
     5881 gcctcctcca cgacgtcctc gaggactcgt cgatgacgaa ggaacgtgtc ggcgagctgt
     5941 tcgggcccga cgtcgcgcac ctcgtggacg gggtcacgaa gatcggcaag tacgcgttca
     6001 cgtccaagga ggcgcagcag gccgagacgt tccggaagat gctcctcgcg atgacggacg
     6061 atctgagggt gatcctcgtc aagctcgcgg accgcctcca caacatgcgg acgctcgaac
     6121 acctgcccga ggccaagcag cgcaccatcg cggccgagac gatggagatc tacgctccgc
     6181 tcgcgaaccg gctcgggatg ggcaagatga agggcgagct cgaggacctc tcgttccggt
     6241 tcctctaccc cgaggagttc gccgcgctgc agacggcggt cgacgagcgg atgaaggcct
     6301 cggccgccaa cgtcgagaag ttccggcagg agctcatcgc aaaaacgcag gccgcgggca
     6361 tcgaggcgga gatcacgggc cgcgtgaagc gctactggtc cgtgcgccag aaactcatcc
     6421 ggcaggccat cccgctcgag cagctctacg acgtcctcgc gttccggatc ctcgtctcga
     6481 gcatcccgga ctgctacacg gtcctcggaa tcgtgcacca ggcctggcgg cccgttcccg
     6541 ggcgcatcaa ggactacatc gcgatgccga agcggaactt ctaccagtcc ctccacacga
     6601 cgctcgtccc cgaaggagcc gtgccgttcg aggtccagat ccgcacgcgg gagatggacc
     6661 tcatcgccga gaacgggatc gcggcgcact ggaagtacaa ggaaggggag tcgagccgcg
     6721 cccaggaggc gcacgtcggc ctgctccgcc agatcttcga gacgacgcgc gacgtgtcgg
     6781 atccgcgcga gttcctctcg tccctgcgca tcgacctgta cccggacgac gtgtacatct
     6841 tcagcccgaa gggcgccgtc ttcgcctttc cgcgcggggc gacgccggtg gacttcgcgt
     6901 atcgcgtcca cacggacgtc ggccaccaca cgacgggcgc gcgcgtgaac gggcggctcg
     6961 tgcccctcaa gaccccgctc gcgaacgggg acatcgtcga gatcctgacg tcgccctcct
     7021 cccagccctc gcgcgactgg ctcaatttcg tgctcacggc gcgcgcgaag aacaagatcc
     7081 gcgccttcat ccacacggcc gagaaggaga agtccgtcga gatcgggcgg cgcctcctgg
     7141 accgcgagct caagaagtgg aagaagagcc tcccgaagct cctcgaggcg aaggcgttcg
     7201 agcccttcct cggggacttc ggcgtctcga aggtggacga cctcatcgcc gaagtcggct
     7261 tcggcaagct ggcgccgcgc gccgtcgtcg ccaagctcct gcccgaggcg ggcggcgagg
     7321 cgcaggcccc tcagccctcg cgggtggccg cgataggcaa cgccgtccgc cgggtgctgc
     7381 cgttcgggtc ggcgccgggg atccgcgtga aaggggagaa cgacctcctc gcgacgctcg
     7441 cgaagtgctg ccagcccgtt ccgggcgagg agatcgtggg ctacgtgtcc cgcggccggg
     7501 gggtctcggt gcactccgtg gggtgcccga acgtgcggag cctcctcttc gacccgagcc
     7561 gggagatcgc cgttcagtgg gagacggcga aggaccagtc cgtcacggtg gacctcgaga
     7621 tccacaccga agaccgccag ggcatgctcg cgcgcatcac gcagctcctc tcggccgcga
     7681 actcgaacat ccgttcgatc gaggcccgaa cgagcgggga cggccgcgcg acgatagacg
     7741 ccagcgtcac gacgcaggat cgacggcacc tggagcgcct cctcgtgggc ctgcgcgcgc
     7801 ttccgggcgt cacgggcgtg aggcgcaagc tccacacctc ggacgccgag gccggctgaa
     7861 gctggccccg aagggtgtcc tttttccgcg cgatcgaccg gtgcggccgg aggtcccccg
     7921 gagtgcggtg ggccgggggc cggacgaagc ttcgcctcta cccgcgcttt cctgagttcc
     7981 ggatggggcg gacttgtacc acaaacgatt ccgggtcgca agggaaatcg cacattcgca
     8041 acgacttgac actacccggg gtgggacgct caaaataagg cgagtcaatc tcttttcacg
     8101 gcggaccgtg tctcgcgttc cggggtagga ggtcagcaga ccgtgctgtt ccgcaaggcg
     8161 aaaaacctcg tcgggctcga catcggctcg tcggccgtca agctggtcga gttgaaggag
     8221 ggcaaggagg gcacgttcca cctcgtgaag gcgggactgg agaccctctc gcccgaagcc
     8281 atcgtcgacg gggccatcat ggactcgtct ctcgtcgtcg agaccatcac ccggctcctc
     8341 gcgtccacgg gcgtcaagaa cccgaacgtc gccgtgtcgg tctcgggtca ctccgtcatc
     8401 gtcaagaaga tcatgcttcc gacgatgccg gaggaggagc tctcggaatc catccgctgg
     8461 gaggccgagc agtacgtccc gttcgacatc aacgacgtgt acctcgacta cgtggtcctc
     8521 gaccccggcg ccgtcggcga gacgatgggc gtcctgctcg tggcggccaa gaaggaaaag
     8581 gtcgacgact tcaagaacgt cgtcacccag gcggggcgca cgccgtcgct cgtggacgtc
     8641 gacgcgttcg cgctccagaa ctgctacgag gtcaactacg gggccgaccc cggccgcgtc
     8701 gtcgcgctcg tgaacatcgg cgcctcggtc atgaacgtga acatcctcgc gcaggggcag
     8761 acggtcttct ggcgcgacat cacgttcggc ggcaaccagt tcacggacgc tctccagaag
     8821 gcctactcgc tcaacttcga gcaggccgag tccctcaaga agggcgagcc ggtcgcgggg
     8881 cagacggcgc agtccatcca gcccgtgctg accggcgtct cggaggacgc ggcggccgag
     8941 ctccagaaga cgctcgactt cttccaggcc acatcgggcg ccgaccgcgt ggactcgatc
     9001 gtcgtgtcgg gcggcggctc gaaagccgcg cactttgagg acgtcctgaa ggacaagttc
     9061 ggcctcccgg tcgaggtcat gaacccgttc cggtcgatct tcaccgacga ccgcgcggtg
     9121 gacgcggcgt ggctctcgga ggcctcgccg gcgctcgcga tcgccgtcgg actcggcgtc
     9181 cggaagatcg gggagtagcc gcatgattcg gatcaacctc ctcgccgaag cccggcccca
     9241 gcgcaagaag aagggcgtct ccgccctcgg cgccggcggg cggctgaaca ccctcctcct
     9301 cggcggggcg atcggcctcg cggttctcgt cggcgtcatc cactactggg tcctccgcag
     9361 ccagatcaag gcccaggacg agaagatccg gatcgcgcag gtcgaggtca cgcgcctcga
     9421 gtccgtcctc aaggaagtga aggactacga ggcgaagaag gcccgcgtcc agcgcaaggt
     9481 cgacctcatc aaccagctca agcagaacca gcgcgggccc gtgcgcctca tggacgaggt
     9541 ctcgcgtgcg ctgcccgacc tcgtgtggat cgagaggatg gagtatcacg gcaacgcgat
     9601 ctcgctcgag ggcaaggcct tcaacccgcc ggccgtcgcg aacttcatcg cgaacctgag
     9661 gctcgtcccc gcgttccagg agcccaagct cgcggacctg tccgcgtgct cgggcgcttc
     9721 ggggtccacg ctctactgct acaagatgtc cttcacgttc tccaacctcg accgcacgca
     9781 gcccgagacg gcggctccgg cgccggcgaa gccggccgag cctccgaaga aggccgagcg
     9841 ggccgcgggc ggcgccgccg cccagggaat gtagggaggg cgcgcgaatg gcttcgctcg
     9901 acctcaagca gctggaaggg aagccctggt actacgggct cggaatcggc ctcgcgatcg
     9961 cgatcgcgct gtacgccgtc gcgtactacc agatcccgaa cttcgccgag atgaagcaga
    10021 cgctcgagaa caagaagaac gagctcgacg cgcttcagca gaagatcacg cagggccgcg
    10081 ccgccgagcg ccggctgccg cagctccgcg aggaggtccg gcggaccgaa ctcgacctcc
    10141 agaggctcct cgcgatcctt ccgacggccc gcaacgccga agagctcatc aagaaggtcc
    10201 aggctctcgc gggccagggc gagctcttca cgaagaagtg gtcgccgaag gagtacatca
    10261 accgcgactt ctacgcggag tggcccatcg acctgcaggt cgacggctcg taccacaacc
    10321 tcgcgctctt cttcgagaag gtcgcgcggt tctcgcggat catcaacatc gaggatctcg
    10381 tgatgaccgg ctatcccgac gcgcggggcg gccgcacgct cggggccaac ttcaccgcca
    10441 agacgttcat ctacctcggc gacacggagc cggccacgcc ggccggtccg aaagccgccc
    10501 cgaaggccgg gccgaagccg gccggcggcg cgaagaagga catggaagga tgagcgcccg
    10561 ccacgccacc ctcctcgcgg ccgcggcctt cgccgtgttc gccgtccccg cggccggacc
    10621 cgccgccgcc cagcagggaa cggccccgcc gccgcctgcc gagacgccga agatcacgga
    10681 ccaggatttc accccgtccg tctacacgta cgaggtcggg gggcgccggg acccgttccg
    10741 gtcgctcctc gtgcgcaacc cctcggagaa ggagcgcgtg cgtccgcccg gcctcgcggg
    10801 cgtgatggtc gacgagctcg agctccaggg cacgatcaag gtcaagcagg gctgggtcgc
    10861 gatgatgcgc ggcccggaca acaagtcgca tctcatccgc aaaggcacga ccctcttcga
    10921 cggcgaggtc accgacgtgt ccgcgacgga agtcacgttc cgccagaacg tgaacgaccc
    10981 gacgaacccg aagccgttcc gcgaagtggt caaggcgctc gcactccaga agaagccgtg
    11041 agcgaaggga cgtccatgac gacgatgctc aatgggtccg gaaaaagaag gggcactccc
    11101 gatcagatgg ggtctggtgg aggaagcatg cgcaacccgt gtgtcctcgc cgcccgagtc
    11161 gcggcgctcg cgctgctcgt ccccgtgctc tccggctgcg cgtcctcggg cgacaacgtc
    11221 gccgcaggaa gctccggaac cacggcgccg gcgccggtcg tcctgaccgg agcgacgctt
    11281 ctcgcggacg gggattcccc ccgcctcctc ctcacaggga acgggccgct cgccccgacg
    11341 ctctacaacc gggaggggtc caccaaggtc gtcgtcgacg tcgcgaacgc ggtggcggcg
    11401 cccggcctcg agcccccgcg ggccgacggc accctgctct cgcgcctcga gatgaagtct
    11461 ttcgtggaga tgggcaagcc ccacatccag ttcgagctca cggcgcgcgt ccccctcgac
    11521 cccgccatct cttcggaatc cgggtcgccc gcgatggccg tcagcctcgt gaaggcgacc
    11581 tcggtcatga cggcctccgc ggccgctccg gcgggacccg cgcccgccgc cgcgtccgcg
    11641 cccgagatcc ccgtcgtcaa ggtcgaggcc gagcccgccg tcaaggtcga ggaagttccc
    11701 gcggccgcga cgacgtcgac gaccgcgatc gccgtcgcgt ccgacgcggc gcccgtcccc
    11761 gtccgccatg ccgcctccgg tccgcgcgcg acgcgtctct ccggagtcgc cgtcgcgcag
    11821 agcggcggtg ggctcgtcgt gacgctctcg ggaaacggct cgttcgccta cgagacgttc
    11881 gccctcgcga accccccgcg ctacgtcgtc gacctccccg gcgtcctcct cgcgacgccg
    11941 aagaagttgc aggacgtccg ccacccggcc gtgacgcgcg tccgtgcgtc ccagttcaag
    12001 ggcggttccg agcccgtcac acgggtcgtg ttcgacctgg cgaatccggt cgagccggcc
    12061 gcgagatccg catcggcggg gctcgcgttc gccttcggga cgggctcctc cgcggtcgcg
    12121 tccgcacccg cggtccggga agcccccgcc ccggtcgtca aggaggccgc ggcaccgctc
    12181 gtgaaggtcg aggctccggc gcctgctctg aacacctccg ctcccgcccc ggcgccgtcc
    12241 gtggccgagg ctcagacggc ccccgccccg gcgcccggca aggtcacggc cgcgcccgag
    12301 aaggtcgccg tcgtcgcggc gcccgccgcc tcgtcctacg agaggca
//
LOCUS       DAOMFY010000099        12240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1607_length_12240_cov_6.792368, whole genome shotgun sequence.
ACCESSION   DAOMFY010000099 DAOMFY010000000
VERSION     DAOMFY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1607_length_12240_cov_6.792368"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLB01_09790"
     CDS             complement(<1..832)
                     /locus_tag="PLB01_09790"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77633.1"
                     /translation="MGARRARVRGPRQGTAPRRHGRRRARRDARAEAEGENLTRRVGL
                     LLPNRFPGLPSYRLPDDAGEPVLGARVAAPFGSALVTGLVADLDPPASPEGTAERDVV
                     ALLEDEPFLPPALVRMLVRAAAYYVVAPGEMLRAAVPGRLLGGSEAVYVAGSAAVGAS
                     VAGIAGDVLSLLLERGEARLPELVEAIGRRGLASALKELVGRGYARIRSEHLRAAAAP
                     SDRAWMARPAAADHPVFARAPKRKALHAHLLALGRPASPEELRAAGGTPAVLAALAKA
                     G"
     gene            complement(718..1359)
                     /locus_tag="PLB01_09795"
     CDS             complement(718..1359)
                     /locus_tag="PLB01_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77634.1"
                     /translation="MRHVLGVAALAVLAAAAALVLTRRLPAPASPRPAAENAGARSGL
                     PTPAERRAIDAAIDAAAAAPPKLTLRPAVDADATDTDEHRDLDAVYGVYHPYFTRGDL
                     DGDGRPDFVQAFVEKGRSGLWFHVAVFFGKADGTFGGPVWVERAISLADGDVTVERSL
                     VVITPDLAGDATRRWRWEPAEHVFVDPDKEPRPVVTDDDVPDETPEQKPRARI"
     gene            complement(1368..1907)
                     /locus_tag="PLB01_09800"
     CDS             complement(1368..1907)
                     /locus_tag="PLB01_09800"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQQ77635.1"
                     /translation="MACSLCKTRKQTVFADGSPDAKIMFIGEAPGADEDAQGVPFVGR
                     AGQLLTRMIEDGMGLPRKSVYIANVLKCRPPDNRNPEPEEIASCRGYLEAQIDLVRPE
                     VLVALGKFAAQFLLETEEGIMRLRGKWGTYRGIPVMPTFHPSFLLRQPGQKKDAWEDL
                     LKVLAKVGLPPPSRRGTKA"
     gene            complement(2202..2435)
                     /gene="rpoZ"
                     /locus_tag="PLB01_09805"
     CDS             complement(2202..2435)
                     /gene="rpoZ"
                     /locus_tag="PLB01_09805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQQ77636.1"
                     /translation="MSQNLPPNVESKFRLVHIASRRAEQLVQGARPKMESKHAKVTRV
                     ALDEVDNDLVRWQLASAEPAVEEVPAIDPLPTE"
     gene            complement(2457..3080)
                     /gene="gmk"
                     /locus_tag="PLB01_09810"
     CDS             complement(2457..3080)
                     /gene="gmk"
                     /locus_tag="PLB01_09810"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQQ77637.1"
                     /translation="MTRGDLFIVSSPSGAGKTTLIRRVLEDPRIGGTLHFSVSHTTRP
                     PRAGETSGVEYHFCDEAAFRRLEAEGGFLEWATVHGNLYGTSRGEVEPRLASGLDVLL
                     DIDVQGARQVRSSVPEAVKVIVFPPSREVLEARLKARASDAPEVVARRLAVAAKEMAE
                     FGEYDYAIINDGLEPAVDELRSIIVARRAGRGRRRERLEAILRTFAP"
     gene            complement(3091..3972)
                     /locus_tag="PLB01_09815"
     CDS             complement(3091..3972)
                     /locus_tag="PLB01_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQQ77638.1"
                     /translation="MKSMTGFGKAAGTLPDGTEVGVVVKGVNHRFLDLGIKLRDEYAS
                     AEPAIRRAVAKVVQRGHVDVLIRTTKPASAAATARIDMEAAAKYAREWREDSLKMGLP
                     ADLPARDLLALPGVVRTDDAGASPDSTFEESLLPIVEQALQDFDAARAREGAALSLTF
                     KDILQRIDSGVALLDAARAGISERIAAALRERIEKLAAGIPLDEGRLAQEVAQLADRA
                     DITEEIDRLKTHLAEARRLLASGGAIGRRLDHLAQELHREVNTSGSKVREAGATKLVL
                     DLKSELEAFKEQVQNVE"
     gene            complement(3969..5858)
                     /locus_tag="PLB01_09820"
     CDS             complement(3969..5858)
                     /locus_tag="PLB01_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ77639.1"
                     /translation="MNDFRRLAGLFKPHRRAAVVAICAMLGVSFFTSSVAYLIGPLFD
                     EVLSAEAKSAVMEHTGKTGVGSLVGEKAALRRTGVARTLDRGLQTLEESFGVTEKGRR
                     LFLPLLLFVAFLLKNLCTFFAEYRFNAVGLAFVRDLRRRVYEKVLGQSGSFFAKHPSG
                     DLIARVTGDVDRIQSLFGTDLADLVQSVATLLGLMVLVVSLSPELTAAALLIAPAIVI
                     PVVLIARRLRHLSKAARERMGDLTGVLSETIRGARVVQAYGAESWESGRFSEVNERTF
                     RLAKKGARVMAFASPLVESASVLTFLLLLGYASTRIASGKMTLGTFLSFGVGLVMMYQ
                     PFKRATRTNLALQQALASARRVFEVLDAPNDVADLAGAPALAVLSREIRFEGVTFAYP
                     GGEPVLHEIDLVLPRGSVTALVGPSGGGKSTLASLLPRFMDPTAGRVTVDGTDLRGVT
                     LASLRAQIGLVTQDVVLFDETIRRNVAYGTPGVPDARVREALAAANALGFVDALPEGL
                     DARVGEGGARLSGGQRQRLAIARALLKDPPILVLDEATSALDAESERAVQQALERLMA
                     GRTTLVIAHRLSTVRRADQLVVIEAGRIVERGSHGDLLATGGLYRRLHDLQVFANGDG
                     GGGVS"
     gene            complement(5855..7003)
                     /gene="lpxB"
                     /locus_tag="PLB01_09825"
     CDS             complement(5855..7003)
                     /gene="lpxB"
                     /locus_tag="PLB01_09825"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQQ77640.1"
                     /translation="MRLLVVAGEVSGDQHASSLLAALRPRVPSLDVFGVGGEGMKAAG
                     ARLLAHQKDLAIIGLVEAFSKIRFARRLIHALVAEASREKADAAVLIDSPDFNLPLAK
                     RLAKAGVPVVFYVSPQVWAWRRRRARTIARIGRAVLVLFGFEKRWYDARGLGERVTWV
                     GHPLVDAAARELARPAAPPPAGVRRVVLMPGSRKGEVKSILPVLRDAVEILRKSARNL
                     EVVLVQADSIPRALFGEIVGESLATWHLVSGDHLSLLRDSDVLLVASGTATVEGLLAR
                     VPMVVVYKVHPVTFWVARRIVKVPHVAMANIVSDDGTGRRTVPELLQGDATAENVAAE
                     AERFLADAALAADTRARLAKGAADLGPPGAGARTAEALLAALGQGKAA"
     gene            complement(7000..7182)
                     /locus_tag="PLB01_09830"
     CDS             complement(7000..7182)
                     /locus_tag="PLB01_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77641.1"
                     /translation="MSLLKDHFVLLVWLAALLAAFLALLWREDARTRPRFFLRTFGAL
                     VVGSVLAAWALSAVAR"
     gene            complement(7179..7448)
                     /locus_tag="PLB01_09835"
     CDS             complement(7179..7448)
                     /locus_tag="PLB01_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77642.1"
                     /translation="MTKRPGEAALLALVAAAFPGVGHALLGQRARGAAFCTIVLLTAA
                     TGLALGPGNDVAALAEYARTFLIVAGIMQALLVLDVFERARGRRG"
     gene            complement(7445..7933)
                     /locus_tag="PLB01_09840"
     CDS             complement(7445..7933)
                     /locus_tag="PLB01_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQQ77643.1"
                     /translation="MEALFTPWRAAYLAGGGVDPGGCLFCELPKHDDAEALVVTRGRL
                     AYAVLNRYPYTNGHVMIAPFAHRSGLDDMTADERHEVVDLAARFEAILRTTYRPHGFN
                     AGLNLGRSAGAGVEHHAHLHIVPRWDGDTNFLTVLGGTRTIPEDLPTTRARLAAAFAR
                     AS"
     gene            complement(7933..8274)
                     /locus_tag="PLB01_09845"
     CDS             complement(7933..8274)
                     /locus_tag="PLB01_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQQ77644.1"
                     /translation="MVGRGEGTTTKADLVEEVAKATDLTKKQAEVIVESVFDGIIASL
                     RSGQKIELRGFGSFRLRQRGARIARNPKRSGVKVMVPPKRVPYFKPGKKLKELINQAA
                     PAAVAGEPDKG"
     gene            complement(8268..9194)
                     /gene="sppA"
                     /locus_tag="PLB01_09850"
     CDS             complement(8268..9194)
                     /gene="sppA"
                     /locus_tag="PLB01_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HQQ77645.1"
                     /translation="MKRSTVAWGVAGIACAFLVLLVAAAFLVSGAVARGGARTDGLGF
                     ALGGRVGVVDLDGVILSADRFRETLKRFHDAANVKAVVVRINSPGGGVAASQELNREI
                     ARFRKETKKPVVVSMESVAASGGYYAAVAADHIVANPGTITGSIGVIIQWVNYGDLLE
                     WAKLRAVTIKSGALKDSGNPTRPITDEDRAYFEDLIQKLRAQFVGAVTEGRKTKLKPG
                     AMDRMADGRVVTGEEALTLGLVDEIGDYRDAVDRAARLAGMSLPAEVWHPRPMRPGLL
                     ELLSGGPSETSIAGRLLSGAPVGGGWNVFFLW"
     gene            complement(9228..10931)
                     /locus_tag="PLB01_09855"
     CDS             complement(9228..10931)
                     /locus_tag="PLB01_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQQ77646.1"
                     /translation="MSPTDLKKTGPKAEEGDTGEFARLLAESERSLKEGELIKGRVLQ
                     VTNDEILVDIGYKSEGMIPRSEFHHVPADKLPHPGQEIEAILERTEGPNGYVVLSYEK
                     ARRGRAWDIVEAAFAANEPVKGRVIDRVKGGLSVDIGVRAFLPGSIADSRPLKNLDVL
                     KGRDLEFRVVSLDKKRGNIVLSRKAILDEVAEVKKKEAASALADGRVLKGIIKNLTDY
                     GAFVDLGGIDGLLHVTDMTWGRLAHPSDAVHVGQEVEVKVLRFDEATGRISLGMKQLK
                     ADPWQDLPAHYPVGARVRGKVVSLTDYGAFVELEEGVEGLIHVSEMSWTKRVKNPSKL
                     LNVGDTIEAVIADVNPETRRLSLSLRATEPNPWEQLAEKFHIGDRIKGTVRNLTDFGA
                     FVEIEDGIDGLIHVSDMSWGRRVKHPSEILKKGDEVQAVLTAIDVENRRISLSIKEFR
                     PNEWEEFASKHQPGDVVDGHVAKVADFGVFVRLPEGLEGLMHVSETPLPRGEKPGNVY
                     HEGDPVRVRILRIEPAEQRIGLSSRDVDQPEAGAHAEAAPSEGDASADEHVHADTEPS
                     E"
     gene            complement(11058..11732)
                     /gene="cmk"
                     /locus_tag="PLB01_09860"
     CDS             complement(11058..11732)
                     /gene="cmk"
                     /locus_tag="PLB01_09860"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQQ77647.1"
                     /translation="MALPLIVAIDGPSGVGKSTVGKSVAARLGVPYLDTGAMYRAVGL
                     AAKRAGVTLPIADPGAVVDLANRLDVRVTGSAGDARTWLGGEDVSKEIRTPEISKYAS
                     AVSAIPGVRRRLAGMQRSLALEGGGVLEGRDIGTKVVPETRHKFFLTATAEVRARRRH
                     DELAARGAPPAYEQVLADMVARDHADSTRADSPLTDDGTYVRVDTSALTAAEVVEQLL
                     RAVEKG"
     gene            complement(11916..>12240)
                     /locus_tag="PLB01_09865"
     CDS             complement(11916..>12240)
                     /locus_tag="PLB01_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77648.1"
                     /translation="RSAQPKSITATGADANTPQDRINADGSVTQRNLGRKDNQFSSLD
                     IRLSRPFDLGAVQLEPIVECFNVFNSKNLKKPETTNLIFNFDGTVQSGLGDARQVQLG
                     LRVIF"
BASE COUNT         1802 a         4268 c         4363 g         1807 t
ORIGIN      
        1 ggccggcctt cgcgagcgcg gcgaggacgg cgggagtgcc gcccgcggcg cgcagctctt
       61 cgggtgacgc cggccgcccg agggcaagaa ggtgcgcatg gagggccttc cgcttcggcg
      121 cgcgggcgaa caccgggtgg tcggccgctg ccggccgcgc catccacgcg cggtcggacg
      181 gcgccgcggc ggcccgcagg tgttccgacc ggatccgtgc gtatcccctc ccgacgagct
      241 ccttgagcgc ggacgcgagg cccctgcgcc cgatcgcctc gacgagctcg ggcagccgcg
      301 cctcgccgcg ctcgagaagc agggagagga cgtcgccggc gatgcccgcg acggacgctc
      361 ccacggccgc gctcccggcg acgtagacgg cttcactccc gccgagcagg cggccgggca
      421 cggccgcgcg cagcatctct ccgggcgcga cgacgtagta cgcggcggcg cggacgagca
      481 tccgcacgag agccgggggc aggaacggct cgtcctcgag aagcgcgacg acgtcccgct
      541 ccgcggtccc ctcgggagac gccggcgggt cgaggtccgc gacgagtccc gtgacgagcg
      601 cgctcccgaa cggcgcggcg acgcgcgcac cgaggacggg ctctcccgcg tcgtcgggca
      661 gccggtacga cgggaggccc ggaaaccggt tcggaaggag gaggccgacc cggcgcgtca
      721 gattctcgcc ctcggcttct gctcgggcgt ctcgtcgggc acgtcgtcgt ccgtgacgac
      781 ggggcgcggt tccttgtcgg ggtccacgaa cacgtgctcg gcgggctccc agcgccagcg
      841 gcgcgtggcg tcgcccgcaa ggtcgggtgt gatcacgacg aggctccgct cgaccgtcac
      901 gtctccgtcc gcgagcgaga tcgcccgctc cacccacacg ggcccgccga acgtgccgtc
      961 cgcctttccg aagaagaccg cgacgtgaaa ccacagtccg ctccggcctt tctccacgaa
     1021 cgcctggacg aaatccgggc ggccgtcgcc gtcgagatcg ccgcgcgtga agtacgggtg
     1081 gtagacgccg taaacggcgt cgaggtcgcg atgctcgtcc gtgtcggtcg cgtcggcgtc
     1141 caccgccggg cggagcgtca gcttcggagg cgccgccgcg gcggcgtcga tcgcggcgtc
     1201 gatcgcgcgc ctctcggccg gcgtcggcag cccggagcgc gctccggcgt tctcggcggc
     1261 cggccggggc gacgccggag ccggcagacg gcgcgtgagc acgagcgcgg ccgccgcggc
     1321 caggaccgca agggccgcga cgcccaggac gtgtctcaag cgcagcgtca cgccttcgtc
     1381 cccctccggg acgggggcgg gagacccacc ttcgcgagaa ccttcaggag atcctcccac
     1441 gcgtccttct tctggccggg ctggcgcagg aggaacgacg ggtgaaacgt cggcatcacg
     1501 gggatcccgc ggtacgttcc ccacttgccg cgcagccgca tgatcccttc ctccgtctcc
     1561 aggaggaact gcgccgcgaa cttgccgagc gcgacgagga cctcgggcct gacgaggtcg
     1621 atctgcgcct cgaggtagcc gcggcaggag gcgatttcct ccggctcggg gttgcggttg
     1681 tcgggcggcc ggcacttgag gacgttcgcg atgtagacgg acttgcgcgg caggcccatg
     1741 ccgtcctcga tcatgcgcgt gaggagctgc cccgcgcgac ccacgaacgg cacgccctgc
     1801 gcgtcctcgt ccgcccccgg cgcctcaccg atgaacatga tcttcgcgtc cggcgagccg
     1861 tcggcgaaga cggtctgctt gcgggtcttg cagagcgagc aggccacgca ggaagagact
     1921 ttctttgcta aggactgcag gtccgccgaa gaagcgctgc gagcggactt cgcagcagcg
     1981 ggataaggcg cgttttcatc gaaggtgtac gaagcggccg gcgcgtgggg cgccgacgcc
     2041 gcagtgactg cttcgcctcg gcccgccgcc ccatcaacct tcagagaaat cttctctttg
     2101 tcttcttctt cttctcttcc ttctctttcc aggtacagct ccgccacccc gaggtcgcgg
     2161 tagtaggcga gaaggtcttt gagcgggtca gacgtggacg atcactccgt cgggagcggg
     2221 tcgatcgccg gcacttcttc cacggccggt tcggcggagg cgagctgcca gcggacgagg
     2281 tcgttgtcga cctcgtcgag ggcgacacgc gtcaccttcg cgtgcttgct ttccatcttc
     2341 gggcgggcgc cctgaacgag ctgctcggcg cgtcgcgacg cgatgtggac gaggcggaac
     2401 ttggattcga cgttcggcgg aagattctga gacatctggg gttagcctcc gtgagatcag
     2461 ggtgcgaaag tcctgaggat cgcctcgagc cgctcgcgcc ggcgcccgcg ccccgcgcgg
     2521 cgcgccacga tgatcgagcg aagctcgtcc accgcgggct cgagaccgtc gttgattatc
     2581 gcatagtcgt actcgccgaa ctcggccatc tccttggccg ccacggccag ccgccgggcc
     2641 acgacttcgg gagcgtcgga ggcgcgcgcc ttgaggcggg cctcgaggac ctcccgcgag
     2701 ggcggaaaga cgatgacttt gacggcctcc ggaaccgacg accggacctg ccgggcgccc
     2761 tggacgtcga tgtcgagaag cacgtcgagt cccgacgcga ggcggggctc gacctccccg
     2821 cgggaggttc cgtagaggtt tccgtggacc gtcgcccact cgaggaaccc gccttcggcc
     2881 tcgaggcggc ggaacgcggc ctcgtcgcag aagtggtact ccacgccgga cgtctccccc
     2941 gcgcgcggcg gcctcgtcgt gtgcgagacg gaaaagtgga gcgtgccccc gatccggggg
     3001 tcctcgagaa cgcgccggat cagagtggtc tttccggccc cggacgggga cgagacgatg
     3061 aagaggtctc cgcgggtcat cgggcgtctc tcattcgacg ttctggacct gttccttgaa
     3121 ggcctcgagc tcggacttca ggtcgaggac gagcttcgtc gcccccgcct cgcgcacctt
     3181 cgagccggac gtgttcactt cgcgatgcag ctcctgcgcg aggtggtcga ggcgccggcc
     3241 gatcgcgccg ccggacgcga ggaggcgccg cgcctcggcg aggtgcgtct tcaggcggtc
     3301 gatctcctcc gtgatgtccg cgcggtccgc cagctgcgcg acttcctgcg cgagccgccc
     3361 ctcgtccaac gggatcccgg ccgccagctt ctcgatacgc tcgcgaagcg cggcggcaat
     3421 gcgctcggag atgccggcac gggccgcgtc gagaagcgcc accccggagt cgatcctctg
     3481 aagaatgtcc ttgaaagtaa gagagagagc ggcgccttcg cgcgcccgcg cggcatcgaa
     3541 gtcctgaagc gcctgctcga cgatcggaag aagagattct tcgaaagtgg aatcaggcga
     3601 ggcgcccgcg tcgtccgtcc gcacgacccc cggcagcgcg aggaggtccc tcgccgggag
     3661 atccgccggc aatcccattt tcaaagaatc ctctctccac tccctcgcat atttggccgc
     3721 cgcctccatg tcgatcctgg ccgtggccgc cgcgctcgcg ggtttcgtgg tccggatcaa
     3781 cacgtcgacg tgacccctct gcacgacctt cgcgacggcc cggcggatcg caggctccgc
     3841 cgaggcgtac tcgtcgcgca gcttgatgcc gaggtcgagg aagcggtggt tcacgccctt
     3901 cacgacgacg cccacctcgg tgccgtccgg cagcgtcccg gccgccttgc cgaacccggt
     3961 catcgatttc atgagacccc tcccccgccg tctccgttcg cgaagacctg caggtcgtgg
     4021 aggcgacgat acaaccctcc cgtcgcgagg aggtcgccgt gcgagccgcg ctcgacgatg
     4081 cggcccgcct cgatcacgac gagctggtcg gcgcggcgga ccgtggagag gcggtgcgcg
     4141 atcacgagcg tcgtccgccc cgccatgagg cgctcgagcg cctgttgcac ggcgcgctcg
     4201 ctctcggcgt cgagggccga cgtcgcctca tcgaggacga ggatcggcgg atccttcagg
     4261 agcgcccgtg cgatcgcgag ccgctgccgc tgcccccccg acagccgggc gccgccctcg
     4321 cccacgcgcg cgtcgagccc ctcgggaagc gcgtccacga agccgagcgc gttcgcggcc
     4381 gcgagcgcct cgcggacgcg cgcgtccgga acgcccggcg tcccgtaggc cacgttgcgg
     4441 cggatggtct cgtcgaagag gacgacgtcc tgcgtcacga gaccgatctg cgcccgcagc
     4501 gaggccaggg tcaccccgcg caggtccgtc ccgtccacgg tcacgcggcc ggcggtgggg
     4561 tccatgaaac gcgggagcag cgacgcgaga gtggacttgc cgccgcccga cggcccgacg
     4621 agggccgtga ccgagccacg cgggaggacg aggtcgatct cgtgcaggac cggctcgccg
     4681 ccgggatacg cgaacgtcac gccctcgaag cggatctcgc gggagaggac ggcaagcgcc
     4741 ggcgcgcccg caaggtcggc cacgtcgttg ggggcgtcga ggacctcgaa gacgcgtcgc
     4801 gccgaagcga gggcctgctg gagcgcgagg ttcgtgcggg tcgccctctt gaacggctgg
     4861 tacatcatga cgaggccgac gccgaacgag aggaacgtcc cgagggtcat cttcccggac
     4921 gcgatgcgcg tggacgcata gccgaggagg aggaggaagg tgaggacgga cgcggactcg
     4981 acgagcggcg aggcgaaggc catcacgcgc gcgcccttct tcgcgaggcg gaacgtgcgc
     5041 tcgttcacct cggagaaacg gccggactcc cagctctcgg cgccgtaggc ctgcacgacg
     5101 cgggccccgc ggatcgtctc ggacaggacg cccgtcaggt cgcccatccg ctcgcgcgcc
     5161 gccttcgaga ggtggcgcag gcgccgggcg atcaggacga ccgggatcac gatggcgggc
     5221 gcgatcagga gcgcggccgc cgtcagctcc ggcgagagcg acacgacgag aaccatgagg
     5281 ccgagaagcg tcgcgacgga ctgcacgagg tcggcgagat ccgttccgaa caggctctgg
     5341 atgcgatcca cgtcgcccgt cacgcgcgcg atgaggtccc cggacgggtg cttcgcgaag
     5401 aacgagcccg actggccgag gaccttctcg tagacgcgcc gcctcaggtc gcgcacgaac
     5461 gcgagtccga cggcgttgaa gcggtactcc gcgaagaacg tgcagaggtt cttgaggagg
     5521 aatgcaacga agaggagaag gggcagaaag aggcggcggc ccttttccgt gactccgaag
     5581 gattcttcga gtgtctggag accgcggtcc agcgtgcggg cgacgccggt gcgcctcagg
     5641 gccgccttct cgccgacgag gcttccgacg ccggtcttcc ccgtgtgctc catgacggcg
     5701 ctcttggctt ccgccgacag cacctcgtcg aagagcgggc cgatgaggta cgcgacggag
     5761 gaggtgaaga acgacacccc gagcatcgcg cagatcgcga ccaccgccgc ccggcggtgc
     5821 ggcttgaaga gacccgcgag gcggcggaag tcgttcacgc cgccttcccc tgtccgaggg
     5881 ccgcgagaag cgcctcggcg gttcgtgcgc ccgcaccggg aggtccgagg tcggccgcgc
     5941 ccttcgcgag gcgcgcgcgc gtgtcggccg cgagcgctgc gtccgcgaga aaccgttccg
     6001 cctcggccgc gacgttctcg gcggtcgcat cgccctggag gagctcgggc acggtccgcc
     6061 ggcccgtccc gtcgtccgag acgatgttcg ccatcgcgac gtgcggaacc ttcacgatcc
     6121 ggcgcgcgac ccagaacgtg acgggatgga ccttgtagac gacgaccatc gggacccggg
     6181 cgaggagccc ctcgaccgtc gccgtgcccg acgcgacgag gaggacgtcg gaatctctga
     6241 gaagggaaag atggtcgccg gaaaccaggt gccaggtcgc cagcgactct ccgacgatct
     6301 ccccgaaaag agcccgagga atcgagtcgg cctgcacgag cacgacctcg aggttgcgcg
     6361 cgctctttct gagaatctcg acggcgtcgc gcagaaccgg aaggatggat ttgacttcac
     6421 ccttccgtga accaggcatc aggacgacgc ggcggactcc ggccggaggc ggggccgcgg
     6481 gacgcgcgag ctcccgcgcg gcggcgtcga cgagcggatg gcccacccac gtgacgcgct
     6541 ctcccagccc gcgtgcgtcg taccagcgct tctcgaaccc gaagaggacg aggacggcgc
     6601 ggccgatccg ggcgatcgtc ctcgcccgcc gcctccgcca ggcccagacc tgcggcgaga
     6661 cgtagaagac gacggggacg cccgccttcg cgaggcgctt cgcgagcggg aggttgaagt
     6721 cgggcgagtc gatgagaacg gcggcgtccg ccttctcgcg gctcgcctcg gccacgagcg
     6781 cgtggatgag gcgccgcgcg aaccggatct tcgagaacgc ctcgacgagg ccgatgatcg
     6841 cgaggtcctt ttggtgcgcg aggagccggg cgccggccgc cttcatgccc tcgccgccta
     6901 ccccgaagac gtcgagcgac gggacccggg gacgcagggc cgcgaggagc gaggacgcgt
     6961 gctggtcgcc cgagacctcc ccggccacga cgaggagcct caccgggcca ccgccgagag
     7021 cgcccaggcc gcgaggaccg agccgacgac gagcgcgccg aaggtcctca ggaagaacct
     7081 cggccgcgtc cgggcgtcct cgcgccagag gagagcgaga aacgcggcga gcagcgccgc
     7141 gagccagacg aggaggacga agtggtcctt gaggaggctc acccgcgcct cccgcgcgcg
     7201 cgctcgaaca cgtcgaggac gaggagcgcc tgcatgatcc ccgcgacgat caggaacgtc
     7261 cgggcgtact cggcgagcgc cgccacgtcg ttgcccggtc cgagggcgag gcccgtcgcc
     7321 gcggtcagga gaacgatcgt gcagaaggcc gcgccgcggg cgcgctggcc gaggagcgcg
     7381 tgtccgacac ccgggaacgc cgccgcgacg agagcgagga gggcggcctc tcccggccgc
     7441 ttcgtcacga cgcgcgcgcg aacgcggccg cgaggcgggc ccgcgtcgtg ggcaggtcct
     7501 cggggatcgt gcgcgtgccc ccgaggacgg tgaggaagtt cgtgtcgccg tcccagcgcg
     7561 ggacgatgtg caggtgggcg tggtgctcga cgcccgcccc ggcgctgcgc ccgaggttga
     7621 gcccggcgtt gaacccgtgc gggcggtacg tcgtgcggag gatcgcctcg aagcgcgcgg
     7681 ccaggtcgac gacctcgtgg cgctcgtcgg ccgtcatgtc gtcgaggccc gagcggtgcg
     7741 cgaacggcgc gatcatgacg tggccgttcg tgtacgggta gcggttcagg accgcgtagg
     7801 cgagacggcc ccgcgtcacg acgagcgcct cggcgtcgtc gtgtttcggg agctcgcaaa
     7861 acaggcagcc gcccgggtcg acgccgccgc ccgcgaggta ggcggcgcgc cagggcgtga
     7921 acagggcctc catcagccct tgtccggctc ccccgccacg gcagccgggg ccgcctggtt
     7981 gatgagctcc ttgagcttct tgccgggctt gaagtacgga acccgcttcg gaggcaccat
     8041 gactttcacg ccgctgcgct tcgggttgcg cgcgatacgg gctccgcgct ggcgcaggcg
     8101 gaagctcccg aaaccgcgca gctcgatctt ctgccccgag cggagcgacg cgatgatccc
     8161 gtcgaacacg gactcgacga tgacctcggc ctgcttcttc gtgaggtcgg tcgccttcgc
     8221 gacctcctcc acgaggtccg ccttcgtggt cgtgccttcg ccgcggccta ccacagaaag
     8281 aacacgttcc atccgccccc caccggagcg ccggagagga ggcgccccgc aatcgaagtt
     8341 tccgaaggcc cgccggagag aagctcgaga agacccgggc gcatcggccg cgggtgccac
     8401 acctctgcag gcagggacat tccggccagg cgcgcagccc ggtccacggc gtcccggtag
     8461 tcgccgatct cgtccacgag gccgagggtc agggcttcct cgcccgtcac gacgcgcccg
     8521 tcggccatcc ggtccatcgc gccgggcttc agcttcgtct tccggccctc ggtgacggcg
     8581 ccgacgaact gggcgcgaag cttctggatg aggtcctcga agtaggcgcg gtcttcgtcc
     8641 gtgatcgggc gcgtcgggtt gcccgagtcc ttgagggcgc cgctcttgat cgtcacggcg
     8701 cggagcttcg cccactcgag gaggtcgccg tagttcaccc actggatgat gacgccgatc
     8761 gaccccgtga tcgttccagg gttcgcgacg atgtggtcgg ccgctacggc ggcgtagtag
     8821 ccgcctgatg ccgcgacgga ttccatcgaa acgacgacgg gcttcttcgt ctccttgcgg
     8881 aatcgcgcga tctcgcggtt gagctcctgc gaggccgcga cgccgccgcc gggcgagttg
     8941 atccgcacga cgaccgcctt gacgttcgcc gcgtcgtgga agcgcttgag agtctcgcga
     9001 aagcggtcgg cggacaggat cacgccgtcg aggtccacga cgccgacgcg gccgccgagc
     9061 gcaaagccca ggccgtccgt gcgggctccg ccgcgcgcga cggcgccgct cacgaggaag
     9121 gccgccgcga cgaggaggac gaggaacgcg caggcgatgc cggcgactcc ccacgcgacc
     9181 gtggagcgct tcatcgggcg gaccccgcgc gtctcacggc ggccccgcta ctcggacggt
     9241 tccgtgtccg cgtgaacgtg ttcgtcggcg gacgcgtcac cctcggaagg cgcggcctcg
     9301 gcgtgggcgc cggcttcagg ctggtcgacg tcgcgcgacg agagcccgat acgctgctcc
     9361 gccggctcga tgcgcaggat gcggacgcgg accgggtcac cctcgtggta gacgtttccg
     9421 ggcttctctc cgcgcggaag cggcgtctcg gagacgtgca tgagcccttc gagtccctcg
     9481 ggcaggcgca cgaagacgcc gaaatccgcg actttcgcca cgtggccgtc cacgacgtcg
     9541 ccgggctggt gcttcgacgc gaactcttcc cattcgttcg ggcggaattc cttgatcgag
     9601 aggctgatcc ggcggttctc gacgtcgatg gccgtgagga cggcctggac ctcgtcgccc
     9661 ttcttgagga tctcggacgg atgcttcacg cgccggcccc aggacatgtc ggacacgtgg
     9721 atgagcccgt cgattccgtc ctcgatctcg acgaaggcgc cgaagtcggt gaggttgcgc
     9781 acggtcccct tgatccggtc gccgatgtgg aacttctcgg cgagctgttc ccacgggttc
     9841 ggctccgtcg cgcgcagcga gagcgagagc cggcgcgtct cgggattcac gtccgcgatg
     9901 acggcctcga tcgtgtcgcc gacgttcagg agcttcgacg ggttcttgac ccgtttcgtc
     9961 cacgacatct cggacacgtg aatgagaccc tcgacgcctt cctcgagctc gacgaaggcg
    10021 ccgtagtcgg tcagggaaac gaccttgccg cgcacccggg cgccgaccgg gtagtgggcc
    10081 gggaggtcct gccacgggtc ggccttgagc tgcttcattc cgagcgagat gcggccggtg
    10141 gcctcgtcga accggaggac cttgacctcc acctcctggc cgacgtggac ggcgtcggac
    10201 gggtgcgcga ggcggcccca cgtcatgtcc gtcacgtgga gcaggccgtc gatcccgccg
    10261 aggtcgacga acgcgccgta gtccgtcagg ttcttgatga tgcccttgag gacgcggccg
    10321 tccgcgagcg cggacgcggc ttccttcttc ttgacctcgg cgacctcgtc gaggatcgcc
    10381 ttgcgggaga ggacgatgtt cccgcgcttc ttgtcgagcg aaacgacgcg gaactcgagg
    10441 tcgcggccct tgaggacgtc caggttcttg agcgggcgcg agtccgcgat cgaaccgggc
    10501 aggaacgcgc ggacgccgat gtcgacggag agcccgccct tcacgcggtc gatgacgcgg
    10561 cccttgacgg gctcgttcgc cgcgaaggcc gcctcgacga tgtcccacgc gcgcccgcgg
    10621 cgcgccttct cgtacgagag cacgacgtag ccgttcgggc cctccgtgcg ctcgaggatc
    10681 gcttcgatct cctggccggg gtgcggcagc ttgtcggcgg gaacgtggtg aaactcggag
    10741 cgcgggatca tcccttcgct cttgtagccg atgtccacga ggatctcgtc gttcgtcacc
    10801 tgaagcacgc ggcccttgat gagctcgccc tccttcagag agcgttcgct ctcggcgagg
    10861 aggcgtgcga attcccctgt atcgccttcc tcagccttgg ggccagtctt cttcaggtcc
    10921 gtgggcgaca tgtcgtcgtg ctccttaatc tcttcgcgat tttccggcct gcctaaatgt
    10981 ccgcgaggca atcgcttaag cggccggaac cggtgatgtt agacggcctg cccggaaacg
    11041 gtcaaccggg gggccgatca gcccttttcg acggccctga ggagctgctc gacgacctct
    11101 gcggcggtca gggcggaggt gtcgacgcgg acgtaggtcc cgtcgtccgt cagcggcgag
    11161 tcggcccgcg tggagtccgc gtggtcgcgc gcgaccatgt ccgcgagaac ctgctcgtag
    11221 gcgggcggcg cgcctctcgc ggcgagctcg tcgtgccgcc gcctcgcccg gacctcggcg
    11281 gtcgcggtca ggaagaactt gtgcctcgtc tccgggacca ccttcgtccc gatgtcacgg
    11341 ccctcgagca cgccgccgcc ctcgagagcg aggctgcgct gcatgcccgc gagcctgcgg
    11401 cgcacgccgg gaatcgcgga aacggcggac gcgtacttcg agatctcggg cgtgcggatt
    11461 tccttcgaga cgtcttcgcc acccagccac gtgcgcgcgt ccccggcgct cccggtcacg
    11521 cgcacgtcga ggcggttcgc gaggtccacc acggcgccgg ggtcggcgat cgggagcgtc
    11581 acgcccgcgc gcttcgcggc aaggccgacc gcccggtaca tcgcgcccgt gtcgaggtac
    11641 gggacgccga ggcgcgccgc gacggacttc ccgacggtcg acttccccac gcccgacgga
    11701 ccgtcgatcg cgacgatcag cgggagggcc atttcctctt cggggccttc ttcccctccg
    11761 cgggggcggg ccgggagggg cgggtgagcg gccgcgccgc gtcgttctct gtcacgggat
    11821 cggctccggc ccccggaagg ggcgaaggcg cccggcgttc cggccgggcg ccctccgtgg
    11881 aatctagcac gcgggggcct gtcccccccg gaccctcaga agatgacgcg gagcccgagc
    11941 tgcacctggc gggcgtcgcc gagaccgctc tggaccgtcc cgtcgaagtt gaagatcagg
    12001 ttcgtcgtct cgggcttctt caggttcttc gagttgaaga cgttgaagca ctcgacgatc
    12061 ggctcgagct ggaccgcgcc gaggtcgaac gggcgagaga gccggatgtc gagcgacgag
    12121 aactggttgt ccttccggcc gaggttgcgc tgcgtgaccg agccgtcggc gttgatccgg
    12181 tcctgcgggg tgttcgcgtc ggcgcccgtc gccgtgatcg acttcggctg cgcggagcgg
//
LOCUS       DAOMFY010000100        12164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1618_length_12164_cov_9.010405, whole genome shotgun sequence.
ACCESSION   DAOMFY010000100 DAOMFY010000000
VERSION     DAOMFY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_12164_cov_9.010405"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..917
                     /locus_tag="PLB01_09870"
     CDS             42..917
                     /locus_tag="PLB01_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQQ77649.1"
                     /translation="MDRPALTDGPGPRAGPPSGGLGRLSFGFLAVSVVSGVFLVPFWT
                     PAAAWDSLERIQGGIAWGFFLRALHAYSSFGVLVTAVLHVVQVLAAKTERQLLAGVWW
                     RSVVLLPVTVAALLGGFVLRGDAEAVAALSIWRRILESLPLAGAEVARLFLGSVPGDL
                     GPAALHHAGTLTLLLWCFTAEHAGRLLPDARGTVLAALVSVALAGIVPLPLGAPPSTA
                     PAHLLGPWSLLGLQGALAVLPPAAGWLAPLAAVLLFGFVRHTDGRARRALLAALVAWC
                     AAYAFFTVRVLLSAR"
     gene            928..2523
                     /locus_tag="PLB01_09875"
     CDS             928..2523
                     /locus_tag="PLB01_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77650.1"
                     /translation="MFRTFAFVAALCAALLGLLTAFSRETRRERAGGRGGDRAGGRVE
                     RCVTCHTKDSEDPGGAHARAALGCASCHLGNPLAFGKERAHEGLEREPGALSTVALTC
                     GREDCHAREAARVVTSPMTRNAGMVAVDRFAFGETASPDGTETITDVLAAGRPTAAQD
                     HLRKLCGGCHLGTRRGNRDDLIHGNGSGCSACHVARRLDEKPRRHPPVDSKVTDDRCL
                     GCHSGSGRIALSYQGLVEVKKDAPFAHAAPDVHQAAGMACIDCHLHTDLMGDGVARGH
                     KEEQVEITCEACHGPVRAGGEAAWLDVKDEISIEIRRREDLLGKGKRGESRPPDAPTR
                     LGSRGAPVLNLLRKSSSSLPPSSSSWVLVRKLDGVELPVKQTPRDRNHTLRGHERLTC
                     SACHAAWVPTCDSCHTSFDPAERQWDFAAGKETAGAWIEASEGFSFAPPLLGVRADGR
                     IVPAAPGMILDIDTSAAGGAKASRRLLAPAEPHTTGKRARTCESCHRASPQPEFEAGT
                     RTGFRGLNAAERKRLAEAKLDRH"
     gene            2702..4909
                     /locus_tag="PLB01_09880"
     CDS             2702..4909
                     /locus_tag="PLB01_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQQ77651.1"
                     /translation="MAQSRREFIKIGGAGVAVAAAGTGLASQWWGLDPDVVHDPGTDG
                     EKIVPTFCELCFWKCGVLAHVKNGRVTKIQGNPEHPLSNGRLCPRGTGGMGLLYDPDR
                     LRKPLLRRKGKRGEQAFEEVSWEAALDFTAEKLDLIRKAHGPEAVALFSHGYGGSFFK
                     TLMAAYGSASIAAPSYAQCRGAREVGFQLTFGSSLGSPENLDLANSRCITLIGSHLGE
                     NMHNTQVQDFAAAIAKGATVIAVDPRFSTAAGKAKYWLPIKPGTDVALLLAWMHVIVN
                     EGLYDREYVDAHTTGLTELKAHLAEKTPEWAFTETTIRPEVIVETARVIAGARPASLI
                     HPGRHTSWYGDDTQRSRAVAILNALVGSWGRRGGFFIPASMGAPKIALPAPATKPRPA
                     PDTPKGVSFPFADETLAHGLRDASIPGTAAYDVKGWIVYGTNLIQALPEPAKTVKAIQ
                     ELDLLVAVDVLPAEIVGWADVVLPECTYLERWDDVGGTPWREPFLAVRQPVVPPMHDS
                     KPGWWIAKQLATRLGHGDFFPYDDAGTMVEGRLAKAGYDVDAVKAKGLVRGKAVPVTT
                     EDGLALTFATPSKKIEIYSKQMEAAGLPPLPVYTHHEEAGSGRFRLLYGRSPVHTFGR
                     TTNNRFLGEVMKDNEVWLNAKIAKERGLASGQNVVLVNQDGVKSPPARLKATERMRPD
                     CVYVVHGFGHTDPGLRFAKGRGIDDNALMSRSKVDPAMGGTGMNVNFVSVEKA"
     gene            4906..5454
                     /locus_tag="PLB01_09885"
     CDS             4906..5454
                     /locus_tag="PLB01_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ77652.1"
                     /translation="MSAAKKRYAMTVDTRQCVGCKACVLACKAENDVPDGFCRDWIVE
                     EVRGEFPLLAAEIRSERCNHCSDAPCVKNCPTGASHVNEGGAVLVTHGKCSGCKACIT
                     ACPYDARFVNPRGYIDKCTFCLHRVQVGVLPACVTVCPTRTLAFGDLNDPESPVAKNL
                     ASRRWKVNHPESGAQPNLYFLL"
     gene            5465..6436
                     /gene="nrfD"
                     /locus_tag="PLB01_09890"
     CDS             5465..6436
                     /gene="nrfD"
                     /locus_tag="PLB01_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQQ77653.1"
                     /translation="MTPQLLETVTTRANAQIDPSLHVWGWEITVYLFLGGIVAGIFVL
                     TAALELASGARPLSRSLRLAPFAALALLSAGMFALWLDLAHRLWAWRFYAAFRPTSPM
                     SWGAWILWLVYPVGLTFGLGSLGSEERGVLRKWVPGVLRSAFEGVLAFSDKRRRSILV
                     AAVLVGTGLGIYTGLLLGTMPSRLAWNSPVLGPLFLASGVSTGAASLLLLRLDEGERK
                     TLVRWDAIAIGAELVLIVLLLLGWATSGEAGRFAARSFLGGPYTAVFWSLVVVTGLLV
                     PLAMEFLESRRHLKFIALVPVLILAGGLALRWILLLAGQASAFRLLP"
     gene            6450..6983
                     /locus_tag="PLB01_09895"
     CDS             6450..6983
                     /locus_tag="PLB01_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQQ77654.1"
                     /translation="MSATMSPPAERPFWNPYVAGVVLGLVLLASFLVMGFGLGSSGAV
                     TRFAVAGAHLVAPKAVETNAYFSQYYGPGKHVLEDWLVFEVLGVFLGGALGAYSAGRM
                     KLGVDKGAHTTTARRLVLAVAGGVLMGFAARLARGCTSGQALTGGAALSLGSWVFMMA
                     VFTGGYLVAPFVRKEWR"
     gene            6980..8164
                     /locus_tag="PLB01_09900"
     CDS             6980..8164
                     /locus_tag="PLB01_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQQ77655.1"
                     /translation="MTFPLYPHGLFGTYAGLLVGTLVGVAFGFVLERAGFGRARNLAA
                     QFYLTDLRVLKVMFSAIVTALVGMTVLAGVGVLDLSLITVPETFLWPQLVGGLLLGAG
                     FIVSGYCPGTGIVAIASGNLDGVFAIGGVMLGSLAFGVGYGPLESFYKSGAMGAVRID
                     TLLGVPAAVVAAAVVAMAVGAFLGGEKLEAIFAPRQGEPVPASPGRVKMRVFSGFAVA
                     SALALVALALPARRTVAAEKSIGKIGAVALAKDLVASPQSWWVVDLRPEAVAEKGRLP
                     GSIVVGPEEKGAAAAANLAATRKLVIYSQGDLASPPEGVKRFAGEIYVLKGGYDAWKA
                     EILTAPKLPENPTAAQSAEFKTRAALYARYTGAAATAGPARSEPRPAAAPAAEPKKGG
                     GC"
     gene            8164..9111
                     /locus_tag="PLB01_09905"
     CDS             8164..9111
                     /locus_tag="PLB01_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQQ77656.1"
                     /translation="MRKKTEEFLRKGYFVRTQRSVIFSVLFMLAAALAQPVLGAVDGL
                     PPVVSPAALAERLAAAPGSVAILDARPSMKAYIAGHIAGAQAVGVDSFRSTAGGVPGT
                     LFPWDMVHFVAHRLGLTSAVPVVVYSEESDIEATYVATVLRTAGVKQVSVLDGGFKRW
                     TAEKRAVTAERKPVAASTEAFTPRPADLIALDEVKKAVETKSAVLLDARPLEAYDKGH
                     LPGAKSRFWMKDIVPAGQPDAGSFRSEAELKVELEAMGVTAATPVIVYCNTGQQASSL
                     FYTLRYRLGYANVRLYNGSWLEWSITPGLPKEVAATPAS"
     gene            9171..10421
                     /locus_tag="PLB01_09910"
     CDS             9171..10421
                     /locus_tag="PLB01_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQQ77657.1"
                     /translation="MKTRIGVALVALLALAAAPALATDGYFQLGYGTAQNGMGGVGVA
                     LSLNTMSPATNPAGNAWVTGYDLGLAVFNPNREFTVTGSPSGYPGTFGLQPGTVKSGS
                     TVFLMPSIGANWKLNDAMTLGVAIYGNGGMNTNYDASVFYAGRTGVDLMQLYVAPTFT
                     YRIGQNHALGVSPVLGWQSFQAEGVGSFAPYSSSPQNLSNNGHDTSLGWGVRIGYLGK
                     LTPWLSFGASYQTEMEMQKFEKYSGLFAEGGDFDIPENWTIGIAVQPTKELTFAFDVQ
                     QINYSNTESIGNPMLPNLVTARLGEDGGAGFGWKNVTAYKFGVQVAASPAVTVRAGYA
                     YEKQPIPESEVLFNILAPGVIEQHITAGVSANVGAKTKLNFAVVRALSKSVTGPNPLE
                     VPGRQTIELKMDQWIFDLGLSIGF"
     gene            10462..10788
                     /locus_tag="PLB01_09915"
     CDS             10462..10788
                     /locus_tag="PLB01_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ77658.1"
                     /translation="MAVLLPARMLERPADVTRLARLFHALSDETRLRIVGKLLGGEQC
                     VCNLTDALAAGQSRLSFHLKTLKDAGLVSDRREGRWVHYALNPDVLQEIKNFVGSVRE
                     GAARCC"
     gene            10840..11649
                     /locus_tag="PLB01_09920"
     CDS             10840..11649
                     /locus_tag="PLB01_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HQQ77659.1"
                     /translation="MAETSIKDVVREKYGEAARRATTGGSACCGGASAIAGAKDPITS
                     NLYDASQTLGLPAAAVSASLGCGNPTALAELHEGDTVLDLGSGGGIDVLLSARRVGSS
                     GKVYGLDMTDDMLALARENQRKAGVTNVEFLKGELESIPLPDASVDVIISNCVINLSA
                     EKDRALAEAFRVLRPGGRFAVSDVVVRGEVPAAVRKNVELWIGCLAGALEEKDFELKL
                     KKAGFTDVEIVPTRIYTSEDARDFLLDVGAAPADLDGKFMSAFVRATKPAV"
     gene            11732..>12164
                     /locus_tag="PLB01_09925"
     CDS             11732..>12164
                     /locus_tag="PLB01_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HQQ77660.1"
                     /translation="MVAGVTIGKLLPGLTGAIRGLEFGAGSQINVAIAVLIWLMVYPM
                     MLKIDFTSILGVRKKPKGLLVTLFVNWLVKPFSMAFLAWLFFKHVFAGLIAPGLADQY
                     IAGAIILAAAPCTAMVFVWSYLTDGDPAYTLVQVSVNDLIML"
BASE COUNT         1743 a         4332 c         4113 g         1976 t
ORIGIN      
        1 gcggccctcg cccgcgtccc gctggtgagg cggggggaat cgtggatcgc ccggctctga
       61 cggacggccc tggcccgcgc gcgggcccgc cgtccggcgg cctcgggcgc ctctccttcg
      121 gcttcctcgc cgtgagcgtg gtctccggcg tctttctcgt tccgttctgg acgccggcgg
      181 ccgcgtggga cagcctcgag aggatccagg gcggcattgc gtgggggttc ttcctccggg
      241 ccctgcacgc gtactcgagt ttcggcgtcc tcgtgacagc ggtcctgcac gtcgtccagg
      301 tcctcgcggc gaagaccgag cgccagctcc tggccggcgt ctggtggcgg tcggtggtac
      361 tgctgccggt gacggtggcg gcgcttctcg gcggcttcgt cctgcgcggt gacgcggaag
      421 cggtggccgc cctctcgatc tggcggcgca tcctcgagtc gctcccgctc gcgggcgccg
      481 aggtcgcgcg cctcttcctc ggatcggtcc cgggcgacct cggtcccgcg gcccttcacc
      541 acgccggtac cctcacgctc cttctctggt gcttcacggc cgagcacgcc gggcgcctgt
      601 tgcccgacgc gcgcggcacc gtcctcgccg cgctcgtctc cgtcgcgctc gccgggatcg
      661 tgccgctgcc gctcggagcg ccgccatcca cggcgcccgc gcaccttctc ggcccgtggt
      721 cgctcctcgg gctccagggc gcgctcgcgg tcctgcctcc tgcggccggc tggctcgctc
      781 cgctcgcggc cgtcctcctc ttcgggttcg tccggcacac ggacggccgt gcgcgtcgcg
      841 ccctgctcgc ggcgctcgtg gcgtggtgcg cggcatacgc gttcttcacg gtccgggtct
      901 tgctctcggc gcggtgaggc aggcctgatg ttccggacgt tcgccttcgt cgcggccctc
      961 tgcgccgcgc tcctcggtct cctcacggcg ttcagccgcg agacgcggcg agagcgggcg
     1021 ggcggccggg gcggggatcg cgcgggcggg cgcgtcgagc gatgcgtgac gtgtcacacg
     1081 aaggattccg aggatcccgg cggcgctcac gcgcgcgcgg cgctcggctg cgcgtcctgc
     1141 catctcggga atccgctcgc gttcggcaag gagcgcgcgc acgaggggct ggaaagggag
     1201 cccggcgctc tctcgaccgt cgccctcacg tgcggccgcg aggactgcca cgcccgcgag
     1261 gcggcgcgcg tcgtcacgtc tccgatgacg cggaacgccg ggatggtcgc cgtcgaccgc
     1321 ttcgcgttcg gggagaccgc atcgccggac ggaacggaga cgatcacgga cgtgctcgcc
     1381 gccgggcgcc cgacggccgc gcaggatcac ctccggaaac tctgcggggg ctgccacctc
     1441 gggacgcggc gcgggaatcg cgacgacctg atccacggca acggctcggg gtgctccgcg
     1501 tgtcacgtcg cgcgaaggct cgacgagaag ccgaggcgcc acccgcccgt cgactcgaag
     1561 gtcacggacg accggtgcct cggctgccac agcggaagcg gacggatcgc cctctcgtac
     1621 cagggactcg tcgaggtgaa gaaagacgcc cccttcgccc acgcagcgcc cgacgtccac
     1681 caggcggcgg ggatggcctg catcgactgc cacctccaca cggacctcat gggcgacggc
     1741 gtcgcccgcg gtcacaagga agagcaggtc gagatcacct gcgaggcgtg ccacgggccg
     1801 gtgcgggcgg ggggcgaagc cgcatggctc gacgtgaaag acgaaatcag cattgagatt
     1861 cggagaagag aagatcttct tggaaagggg aagaggggag agagccgccc gcccgacgca
     1921 ccgacgcgcc tcgggagccg tggcgccccc gttcttaacc ttctaagaaa atcttcctcc
     1981 tctcttcctc cgtcttcgtc ctcctgggtt ctcgtgcgaa agctcgacgg cgtcgaactg
     2041 cccgtgaagc agacgccgcg cgaccgcaat cacacgctcc gcgggcacga gcgcctgacg
     2101 tgcagcgcgt gccacgcggc gtgggtgccg acgtgcgact cgtgccacac gtctttcgat
     2161 cccgccgaga ggcagtggga cttcgcggcg gggaaggaga cggccggcgc ctggatcgag
     2221 gcgtccgagg gcttctcgtt tgcgccgccg ctcctcgggg tccgcgccga cgggcgcatc
     2281 gtcccggccg cgccggggat gatcctcgat atcgacacgt cggcggccgg cggggcgaag
     2341 gcttcgcgtc gcctcctggc gccggccgag ccgcacacga cggggaagag ggcccgaacc
     2401 tgcgagagct gccatcgcgc ctcgccgcag ccggagttcg aggccggcac ccggacgggc
     2461 tttcgcggcc tgaacgcggc cgagaggaag cgtctggcgg aagcgaaact cgaccggcac
     2521 tgagctcctg gctgaaacgc cctgtttcaa acgaggccat ctgcggctgc gacatgttgt
     2581 cgcgcgctaa gacgtgacgt ttcgtgtcgc cctgcttttc ttcactttcc aaggaatctc
     2641 tctctggccc gcttcttgta atggggagtc acaaacgggc atccgcccgt ctggagaaga
     2701 gatggcccag agtcggcgag agttcatcaa gatcgggggc gcgggcgtcg cggtcgccgc
     2761 cgccggcacc ggcctcgcct cgcagtggtg gggtctcgac cccgacgtcg tccacgaccc
     2821 cggtacggac ggcgaaaaga tcgttccgac gttctgcgag ctctgtttct ggaagtgcgg
     2881 cgtcctcgcg cacgtcaaga acggccgcgt cacgaagatc cagggcaatc cggagcaccc
     2941 gctctcgaac gggcggctct gcccgcgcgg aacgggcggc atgggcctcc tctacgatcc
     3001 ggaccggctc aggaagccac tcctgcgccg caagggcaag cgcggggagc aggcgttcga
     3061 ggaagtctcc tgggaggccg cgctcgactt caccgccgaa aagctcgacc tgatccgcaa
     3121 ggcgcacggc cccgaggccg tcgcgctctt ctcgcacggg tacggcggct cgttcttcaa
     3181 gacgctcatg gccgcatacg gctcggcgtc gatcgcggcg ccgtcctacg cccagtgccg
     3241 cggcgcgcgc gaggtcggct tccagctgac gttcggctcg tccctcggct cgccggagaa
     3301 cctcgacctc gcgaactcgc gctgcatcac gctcatcggc tcgcacctcg gcgagaacat
     3361 gcacaacacc caggtgcagg acttcgcggc cgcgatcgcg aagggcgcga cggtcatcgc
     3421 cgtcgacccg cgcttctcga cggccgccgg caaggcgaag tactggctcc cgatcaagcc
     3481 gggaaccgac gtcgccctcc ttctcgcgtg gatgcacgtg atcgtgaacg aggggctcta
     3541 cgaccgcgaa tacgtggacg cccataccac gggcctgacg gagctgaagg cgcacctcgc
     3601 cgaaaagacg cccgagtggg ccttcaccga gacgacgatc cgccccgagg tcatcgtcga
     3661 gacggcgcgc gtcatcgcgg gcgcgcggcc ggcgtcgctc atccaccccg ggcggcacac
     3721 gagctggtac ggggacgaca cgcagcggag ccgcgcggtc gccatcctga acgcgctcgt
     3781 cggcagctgg ggccgccgcg gcggtttctt catcccggcc tcgatgggcg cgccgaagat
     3841 cgcgctcccg gcccccgcga cgaagccgcg tcccgccccc gacacgccga agggcgtctc
     3901 cttcccgttc gcggacgaga cgctcgcgca cggcctgcgc gacgcctcga tcccgggcac
     3961 ggccgcgtac gacgtcaagg gctggatcgt ctacggcacg aacctgatcc aggcgcttcc
     4021 cgagcccgcg aagaccgtca aggcgatcca ggagctggac ctcctcgtcg ccgtggacgt
     4081 cctgccggcc gagatcgtcg gatgggcgga cgtcgtgctc ccggaatgca cgtacctcga
     4141 gaggtgggac gacgtcggcg ggacgccctg gcgcgagccg ttcctcgccg tcaggcagcc
     4201 ggtcgtgccg ccgatgcacg acagcaagcc gggctggtgg atcgcgaagc agctcgcgac
     4261 gcgcctcggc cacggggact tcttcccgta cgacgacgcc ggaacgatgg tcgagggccg
     4321 cctcgcgaag gccggctacg acgtcgacgc cgtgaaggcg aaaggcctcg tccgcggaaa
     4381 ggccgtgccc gtgacgaccg aggacggcct cgccctcacg ttcgcgacgc cgtcgaagaa
     4441 gatcgagatc tactcgaagc agatggaggc cgcgggcctt ccgccgctgc ccgtctacac
     4501 gcaccacgaa gaggccggct ccggccggtt ccggctgctc tacggccgct ctcccgttca
     4561 cacgttcggg cgcacgacga acaaccgctt cctcggcgag gtcatgaagg acaacgaggt
     4621 gtggctcaac gcgaagatcg cgaaggagcg cggcctcgcg agcggccaga acgtcgtcct
     4681 cgtgaaccag gacggggtga agtccccgcc ggcgcggctc aaggccaccg agcggatgcg
     4741 ccccgactgc gtctacgtcg tccacggctt cgggcacacc gacccggggc tcaggttcgc
     4801 gaagggccgc gggatcgacg acaacgcgct catgtcgcgc tcgaaggtcg accccgcgat
     4861 gggcggcacc ggcatgaacg tcaacttcgt gtccgtggag aaggcatgag cgccgccaag
     4921 aaacgctacg cgatgacggt ggacacgcgg cagtgcgtcg ggtgcaaggc ttgcgtcctg
     4981 gcctgcaagg ccgagaacga cgtcccggac ggcttctgcc gcgactggat cgtcgaggag
     5041 gtgcgcgggg agttcccgct cctcgccgcc gagatccggt ccgagcgctg caaccactgc
     5101 tccgacgccc cgtgcgtgaa gaactgcccg accggcgcga gccacgtgaa cgaaggcggc
     5161 gccgtcctcg tgacgcacgg caagtgctca ggctgcaagg cgtgcatcac ggcgtgtccg
     5221 tacgacgcgc gcttcgtgaa ccccaggggc tacatcgaca agtgcacgtt ctgcctccac
     5281 cgcgtgcagg tgggcgtcct gcccgcgtgc gtgaccgtct gcccgacccg gacgctcgcc
     5341 ttcggcgacc tgaacgaccc cgaatcgccg gtcgcgaaga acctcgcgtc ccgccgctgg
     5401 aaggtcaacc accccgagag cggcgcgcag ccgaacctct acttcctgct gtgagggcct
     5461 cacgatgacg ccgcagcttc tcgagaccgt cacgacccgc gcgaacgcgc agatcgaccc
     5521 gtccctccac gtctggggct gggagatcac cgtctacctc ttcctcggcg ggatcgtcgc
     5581 ggggatcttc gtcctcacgg ccgcgctcga gctcgcctcg ggcgcccggc cgctttcgcg
     5641 ttccctgcgc ctcgcccctt tcgccgcgct cgcactcctc tcggccggca tgttcgcgct
     5701 gtggctcgac ctcgcgcacc ggctgtgggc gtggcgcttc tacgccgcct tccgcccgac
     5761 gtcgccgatg tcgtggggcg cgtggattct gtggctcgtc tatccggtgg gactgacgtt
     5821 cggcctgggg tctctgggga gcgaagagcg gggagttctt cgaaagtggg tgccgggagt
     5881 cctcagaagc gctttcgaag gagttcttgc tttctccgac aagaggcgcc ggtcgattct
     5941 cgtcgccgcc gtgctggtcg ggacgggcct cgggatctac acgggcctgc tcctcggcac
     6001 gatgccgtcg cgcctcgcgt ggaacagccc ggtcctcggg cccctgttcc tcgcgtcggg
     6061 cgtctcgacc ggggcggcgt cgctccttct cctgagactc gacgagggcg agcggaagac
     6121 gctcgtgcgc tgggacgcga tcgcgatcgg tgccgagctc gtcctcatcg tcctccttct
     6181 cctcggctgg gcgacgtcgg gcgaagcggg ccggttcgcg gcgcggagct tcctcggcgg
     6241 cccgtacacg gccgtcttct ggtctctcgt cgtcgtcacg gggctcctcg tgccgctcgc
     6301 catggagttt ctcgagtccc ggcggcatct gaagttcatc gccctcgtcc cggtcctgat
     6361 cctcgccggc ggcctcgcgc tgcgctggat cctcctcctg gccggccagg cctccgcctt
     6421 ccggctcctc ccctgaagag gtgcgcacga tgtctgcaac gatgtctccg cccgccgaac
     6481 gccccttctg gaatccctac gtcgcggggg tcgtgctcgg cctcgtcctc ctcgcgtcgt
     6541 tcctcgtcat gggcttcggc ctcggctcgt ccggcgccgt cactcgtttc gcggttgccg
     6601 gcgcccatct cgtcgccccg aaggcggtcg agacgaacgc gtacttctcg cagtactacg
     6661 ggcccggcaa gcacgttctc gaggactggc tcgtgttcga ggtcctcggc gtcttcctcg
     6721 gcggggccct cggcgcctac tcggctggcc gcatgaagct cggcgtcgac aagggtgctc
     6781 acacgacgac cgcgcggcgc ctcgttctcg ccgtggcggg cggcgtcctg atgggcttcg
     6841 cggcgcgcct cgcgcgcggc tgcacgtcgg gccaggcgct cacgggcggc gccgcgctct
     6901 cgctcggctc gtgggttttc atgatggcgg tcttcacggg cggctacctc gtcgccccgt
     6961 tcgtcagaaa ggaatggcga tgacgtttcc tctctacccc cacgggctct tcgggacgta
     7021 cgccgggctc ctcgtcggga cgctcgttgg tgtcgcgttc gggttcgtcc tcgagcgggc
     7081 gggcttcggc cgcgcgcgta acctcgcggc ccagttctac ctgacggacc tgcgcgtcct
     7141 gaaggtcatg ttcagcgcga tcgtcacggc tctcgtcggg atgacggtcc tcgcgggcgt
     7201 cggcgtcctg gacctctcgc tgatcacggt gcccgagacg ttcctttggc cccagctcgt
     7261 cggcggcctc ctgctcggcg ccggcttcat cgtgtcgggc tactgccccg ggacgggcat
     7321 cgtcgcgatc gcgtcgggca atctcgacgg cgtcttcgcg atcggcggcg tcatgctggg
     7381 ctcgctcgcg ttcggcgtcg gctacggccc tctcgagagc ttctacaaga gtggcgcgat
     7441 gggggcggtg cggatcgata cgctgctcgg cgttcccgcc gcggtcgtcg cggccgccgt
     7501 cgtcgcgatg gccgtcgggg cgttcctcgg cggcgagaag ctcgaggcga tcttcgcgcc
     7561 gcgacagggt gagcccgtcc cggcctcgcc cggccgcgtg aagatgcgcg tcttctcggg
     7621 cttcgcggtc gcctccgccc tcgcgctcgt cgccctcgcg ctcccggcgc gccgtacggt
     7681 agcggccgag aagtcgattg gaaagatcgg cgctgtcgcg ctcgcaaagg acctcgtcgc
     7741 ctccccgcag tcgtggtggg tcgtcgacct ccgccccgag gccgtcgccg agaagggccg
     7801 actgcccggc tccatcgtcg tcggccccga ggagaagggc gccgcggcgg cggcgaatct
     7861 cgccgcaacg cgcaagctcg tcatctactc ccagggcgac ctcgcctcgc cgccggaggg
     7921 cgtcaagcgc ttcgcgggcg agatctacgt cctgaagggc gggtacgacg cgtggaaggc
     7981 cgagatcctc acggccccga agctcccgga gaacccgacc gccgcgcaga gtgccgaatt
     8041 caagacgcgc gcggcgctct acgcccgcta cacgggcgcc gccgcaaccg cggggcccgc
     8101 gcgttccgaa ccgcgtcccg ccgccgcacc ggccgccgag ccgaagaagg gcgggggctg
     8161 ctgatgcgaa agaagaccga agagtttctt agaaaggggt atttcgtgag aacacaacgc
     8221 agcgtcattt tctccgtcct tttcatgctg gctgcggccc tcgctcagcc cgtcctcggg
     8281 gcggtcgacg ggctcccgcc ggtcgtttcc cccgccgctc tcgcggaacg tctcgccgcc
     8341 gcccccggca gcgtggcgat cctcgacgcc cggccgtcga tgaaggccta catcgcgggc
     8401 cacatcgcgg gcgcgcaggc cgtcggtgtc gacagcttcc ggtcgacggc cggcggcgtc
     8461 ccggggactc tcttcccgtg ggacatggtc catttcgtcg cgcaccggct cggactcacg
     8521 agcgccgtcc ccgtcgtcgt ctactccgag gagagcgaca tcgaggcgac gtacgtcgcc
     8581 accgtgctcc ggacggccgg cgtcaagcag gtctcggtcc tcgacggcgg cttcaagcgc
     8641 tggacggccg agaagcgcgc ggtcacggcg gagcgcaagc ccgtcgccgc ctcgaccgag
     8701 gccttcacgc cccggccggc cgacctgatc gcactcgacg aagtcaagaa ggccgtcgag
     8761 acgaagagcg ccgtccttct cgacgcccgt cccctcgagg catacgacaa gggccacctg
     8821 ccgggcgcga agtcgcgctt ctggatgaag gacatcgtcc cggccgggca gcccgacgcc
     8881 ggctccttcc gctccgaggc cgagctcaag gtcgagctcg aggccatggg cgtgacggcg
     8941 gcgacgcccg tcatcgttta ttgcaacacc ggccagcagg cttcgtcgct cttctacacg
     9001 ctgcgctacc gcctcggcta cgccaacgtc cgtctctaca acggctcctg gctcgagtgg
     9061 tccatcaccc ccggcctgcc gaaggaagtc gccgccacgc ccgcgtcctg aacaaacgcc
     9121 atcgatgaat cgcggtcccc gcggggtccg cctgtgagag gagtgcccag atgaagaccc
     9181 ggatcggcgt cgcgctcgtc gcgctgctcg ccctcgccgc cgcccccgcc ctcgcgacgg
     9241 acggctactt ccagctcggc tacggaaccg ctcagaacgg aatgggcggt gtgggcgtcg
     9301 cgctgtctct caacacgatg tccccggcga cgaaccccgc cgggaacgcg tgggtgacgg
     9361 gctacgacct gggcctcgcc gtcttcaacc cgaaccgcga gttcacggtc acggggtcgc
     9421 cctcgggcta tcccggcacg ttcggcctcc agccgggcac cgtgaagagc ggttcgaccg
     9481 tgttcctcat gccgtcgatc ggcgcgaact ggaagctgaa cgatgcgatg acgctcggcg
     9541 tcgcgatcta cggcaacggc ggcatgaaca cgaactacga cgcgtccgtc ttctacgcgg
     9601 gccggaccgg cgtggatctc atgcagctgt acgtcgcccc gaccttcacg tacaggatcg
     9661 gccagaacca cgcgctcggc gtcagcccgg tgctcggctg gcagagcttc caggcggagg
     9721 gcgtcggcag cttcgcgccg tactcgtcct cgccgcagaa cctctcgaac aacggccacg
     9781 acacgtcgct cggctggggc gtgcggatcg gctacctcgg caagctcacg ccgtggctct
     9841 cgttcggcgc ctcgtaccag accgagatgg agatgcagaa gttcgagaag tactcgggcc
     9901 tcttcgccga gggcggcgat ttcgacatcc ccgagaactg gacgatcggc atcgccgtcc
     9961 agcccacgaa ggagctcacg ttcgccttcg acgtccagca gatcaactac tccaatacgg
    10021 agtctatcgg caacccgatg cttccgaacc tcgtgacggc gcgcctcggc gaggacggcg
    10081 gtgccgggtt cggctggaag aacgtgacgg cctacaagtt cggcgtccag gtcgccgcaa
    10141 gcccggccgt cacggtccgc gcgggctacg cctacgagaa gcagcccatc ccggagtccg
    10201 aggtcctctt caacattctc gctcccggcg tcatcgagca gcacatcacc gcgggtgtca
    10261 gcgcgaacgt gggtgcgaag acgaagctca acttcgcggt cgtccgcgcg ctctcgaagt
    10321 ccgtcaccgg cccgaacccc ctcgaagtcc ccggcaggca gacgatcgag ctgaagatgg
    10381 accagtggat cttcgacctc ggcctttcga tcgggttctg aggggtcttg acatataaag
    10441 atttcttgat atattgacgt catggcggtt ctcctcccgg cccgcatgct cgaacgccct
    10501 gccgacgtga cgcggctcgc gcggctcttt catgccctgt cggacgagac ccgccttcgg
    10561 atcgtgggaa agctcctggg cggcgagcag tgcgtctgca acctcacgga cgccctcgcc
    10621 gcgggccagt cgcgcctctc gttccacctg aagacgctca aggacgccgg tctcgtctcc
    10681 gaccggcggg aggggcgctg ggtgcactac gcgttgaacc cggacgtcct gcaggagatc
    10741 aagaatttcg tcggatccgt gcgggaaggc gccgcgcgct gctgctgagg ctttcgcgtg
    10801 acggatacat caagaaccat tgatgagaag ggggacccca tggcggaaac gagcatcaag
    10861 gacgtcgtcc gggagaagta cggagaggcc gcgcggcgcg cgacgacggg cggcagcgcc
    10921 tgctgcggcg gcgcatcggc gatcgcgggg gcgaaggacc cgatcacgtc gaacctctac
    10981 gacgcctcgc agacgctcgg cctcccggcg gcggcagtct cggcctcgct cggctgcggc
    11041 aatccgaccg ctctcgccga gcttcacgaa ggcgacacgg tcctcgacct gggttcggga
    11101 ggcggcatcg acgtcctcct gtcggcgcgg cgcgtcggat cctccggcaa ggtctacggc
    11161 ctcgacatga cggacgacat gctcgccctc gcgcgggaga accagcggaa ggcgggcgtc
    11221 acgaacgtcg agttcctcaa gggcgagctc gagagcattc cgcttccgga tgcgagcgtc
    11281 gacgtgatca tctcgaactg tgtcatcaac ctctccgcgg agaaggaccg ggccctcgcg
    11341 gaggcattcc gggtcctgag gccgggcggg cggttcgcgg tgtccgacgt cgtcgttcgc
    11401 ggcgaggttc cggctgccgt ccgaaagaac gtcgagctct ggatcggctg cctcgcaggc
    11461 gctctcgagg agaaggactt cgagttgaag ttgaagaagg cgggcttcac ggacgtcgag
    11521 atcgtcccca cccgcatcta cacgtcggag gacgctcggg acttcctgct tgacgtaggg
    11581 gccgctccgg cggacctgga tggcaagttc atgagcgcgt tcgtgcgggc cacgaagccg
    11641 gcggtgtaag aaagagcacg tgagcgaagc gatcgcaccc aagcgcttga acgtcttcga
    11701 gcgttacctc accctctggg tggctctctg catggtcgcc ggcgtgacga tcggaaagct
    11761 cctgccgggc ctgaccggcg cgatccgcgg cctcgagttc ggcgcgggga gccagatcaa
    11821 cgtcgcgatc gccgtcctga tctggctcat ggtctacccg atgatgctca agatcgactt
    11881 cacgagcatc ctcggggtga ggaagaagcc gaagggcctc ctcgtcacgc tcttcgtgaa
    11941 ctggctcgtc aagccgttct cgatggcctt cctggcctgg cttttcttca agcacgtctt
    12001 cgcgggcctg atcgcgcccg gactcgccga ccagtacatc gcgggcgcga tcatcctggc
    12061 cgccgcccct tgcaccgcga tggttttcgt atggtcgtat ctcacggacg gcgacccggc
    12121 ctacacgctc gtccaggtct ccgtgaacga cctgatcatg ctcg
//
LOCUS       DAOMFY010000101        12143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1621_length_12143_cov_7.722948, whole genome shotgun sequence.
ACCESSION   DAOMFY010000101 DAOMFY010000000
VERSION     DAOMFY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1621_length_12143_cov_7.722948"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PLB01_09930"
     CDS             <1..1022
                     /locus_tag="PLB01_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQQ77661.1"
                     /translation="AWPAREAVLAYAGRADAAVEDALRSGACDAHADAFSTVVEHELM
                     HQETLRYMLHRLPYAEKRAPAGAPAPETAGPSVHADAVLVPAGVATLGAVPGGIPFGW
                     DNEFPKACRFVPAFTVDALPVTNGDFLAFVEAGGYADADVWAPGDAEWLASHGITHPP
                     FWRREGGAWFWVGQFGLVPLPPAWPVWVSHAEASAYARWKGRRLPTEAEYHRAAFGTP
                     EGSERSFPWGDAAPAPEHGNFGGHRDDPVPAGSRPAGASAWGVHELFGNGWEWTSTPF
                     APFEGFMPMATYPRYSADFFDGRHYVLKGASPATPAPLVRRTFRNWFQARYPHVFAKF
                     RCVHP"
     gene            1007..1963
                     /gene="egtD"
                     /locus_tag="PLB01_09935"
     CDS             1007..1963
                     /gene="egtD"
                     /locus_tag="PLB01_09935"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HQQ77662.1"
                     /translation="MRAPVSLAADVKAGFARTPRSLPPKYFYDELGSRLFEAICRLPW
                     YRITAAETRLLRRVAPAVARALAGPVRVVELGSGSGEKLAVVVEALRSGRKRPDVHVV
                     DLSAAAVSASLARLAKIPGVPAKGHRAAYETGLADATAARRGAGACLVLFLGSNIGNF
                     DPPDAAGLLAAVRRRLRPGDAILLGTDLVKPAHELLAAYDDPLGVTAAFNRNVLVRLN
                     RELGADFDVPAWCHRAVWNGRARRVEMHLVSLVRQRVTIPLAAVAAVFEAGETIHTES
                     SHKYRPADVDRLALAAGFRRERRWIDRSARFALTLCRVEEEP"
     gene            2005..2232
                     /locus_tag="PLB01_09940"
     CDS             2005..2232
                     /locus_tag="PLB01_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HQQ77663.1"
                     /translation="MVPAKAAECEECVKIGSEWVHLRTCQACGGTRCCDSSPNRHASK
                     HAKSSGHPVIASAEPGERWLYCYPDDAFVEF"
     gene            2273..2782
                     /locus_tag="PLB01_09945"
     CDS             2273..2782
                     /locus_tag="PLB01_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77664.1"
                     /translation="MKGSLPALVLLAAVAAVGAQSLNPGGKLVETKDGIVFQRNTPAP
                     RPTRERADQPADTGGGLVKVATPPGPTGPAVVDAAYVTFRGTVVRFEKGAAITILDGR
                     TGKERRVFLAKAAVVPDGIKAGDAVAVRVPLEEGSGARTADRVELQKTPPPVDPKSKF
                     TQAKAPATR"
     gene            complement(2790..3182)
                     /locus_tag="PLB01_09950"
     CDS             complement(2790..3182)
                     /locus_tag="PLB01_09950"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HQQ77665.1"
                     /translation="MTPPLAWVLYDGACGFCSRWVPFWKGTLARRGIGIATLQEPWIV
                     ERLEAKGVATENLLDDLRVLVAGSGDVMAGADAYRWAFRRIWWATPLWLLSATPGFRR
                     LFDFCYRAFAENRYRVSKACRLDSPPRG"
     gene            complement(3179..3502)
                     /locus_tag="PLB01_09955"
     CDS             complement(3179..3502)
                     /locus_tag="PLB01_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQQ77666.1"
                     /translation="MAARADSLYRRIWAVAARIPPGRVATYGQVARLAGLPNHARLAG
                     YALHALPPRSAVPWHRVVNAKGEVSPRRDGSEHHVLQRVLLEKEKVRFDRSGRLDLAA
                     LRWKP"
     gene            complement(3504..3950)
                     /locus_tag="PLB01_09960"
     CDS             complement(3504..3950)
                     /locus_tag="PLB01_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77667.1"
                     /translation="MKLPARALVPAVAALLLSCGRPADPVRDCLERMVASAEKRDADA
                     VMARVAESYRGADGGGKADAAALVRRTLAAYESLSLTLSDLAVERGATAAQAKFKVRM
                     SGRPRAVGGLEGLLPRSSRWSFDVRLESEKGGWRIASAAWSRLEDD"
     gene            complement(3985..4134)
                     /locus_tag="PLB01_09965"
     CDS             complement(3985..4134)
                     /locus_tag="PLB01_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77668.1"
                     /translation="MVLCPVALAVGCRKCPIFSFCPVKTVIGDVKVEEEKTPSKGKTR
                     SKKER"
     gene            complement(4204..4773)
                     /locus_tag="PLB01_09970"
     CDS             complement(4204..4773)
                     /locus_tag="PLB01_09970"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HQQ77669.1"
                     /translation="MRLPSHRAAVFAALLAVAAPLALAEDKPAAAPKPPKELSQLSYF
                     LGNWTCTGKTFATPMGPEHAVEAKVGFASALGGYWLAFHYDEAKTAANPMPYHAAGFM
                     GYDTGEKVFIQRCHDSFGGWCNATSKGWAGDVLTFEGPGNMDGKKMTVRDVFTRKSAT
                     EVVHAGAMQGPDGKWVNTDEETCTKAAKK"
     gene            complement(4841..5200)
                     /locus_tag="PLB01_09975"
     CDS             complement(4841..5200)
                     /locus_tag="PLB01_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HQQ77670.1"
                     /translation="MHTLAVVLVGLVALLHAGFLVLEMFLWKTPTGRRVFGLPPDVMA
                     ASAPLAANQGLYNGFLAAGLVWGLLLGPAGFAVKVFFLVCVVVAGVFGAATAKKTILY
                     VQALPGALALAAVWLAR"
     gene            5268..6614
                     /locus_tag="PLB01_09980"
     CDS             5268..6614
                     /locus_tag="PLB01_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQQ77671.1"
                     /translation="MGDPLLLHRLQFAFTILFHYLFPVLTMGLALLIVVMKALGLRKG
                     GERWNDAARFWIRIFGINFAIGVVTGIPMEFQFGTNWARFSRYAGNVIGHTLAMEGVF
                     AFFLESAFLALLVYGEKRLSRVGHFLAAVALFVGSWVSGWFITATNAFMQHPVGHRIA
                     EDGTLRLESFWAFLLNPWAFAEYVHNMTASVVTASFVVASLGAFYVLQGRHLDQAKLF
                     LKLGTTAGLVSSVLLAFPTGDVQAKLVARHQPVALAAMEGRFESGPRAGLNMIGQPNV
                     KERRLDNPVTVPIVLSVLAFGHVTSEVQGLNDFPVSEWPDNVELLYYSFHVMAGLGTL
                     LILVMALAKFLAWRGRLETSSPMLWVLMLAFPFPYIAVLAGWLTTELGRQPWLVYGLL
                     RTAQGSSPTVHAGTTLFTLLGFCGIYTVLAFLFAFLVSLEIARGPGGRPAAETSHA"
     gene            6607..7653
                     /gene="cydB"
                     /locus_tag="PLB01_09985"
     CDS             6607..7653
                     /gene="cydB"
                     /locus_tag="PLB01_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HQQ77672.1"
                     /translation="MHEVWYGTVVFMLTAYVVLDGFDLGAGALHLFVARSDAERRQVL
                     AAIGPFWDGNEVWLLAAGGALFVGFPRVLSSGISGFYFAIFLVLWCLILRGISIELRS
                     HVKDMVWRKTWDAGFAVASVLLPVFFGAALGNLIRGVPLDAEGWFELTLFTDFTPAPP
                     AGILDWYTVAVGVFALAALAAHGGTFLAWKTDGAVQSRSRRAAVALYSAVAVLWAAVT
                     GATSSVNPDLLAALPRRPLALLLAVVAFAGLAGVFVAHARGHDRAAFVSSSAFVAGLS
                     VATAVCVWPVLLRAEPDGARSLTALNAGGDPAGLRTGLAWLAIGLPLVAAYYVFLFRF
                     HRGKVAAAAEGEGY"
     gene            7657..9351
                     /locus_tag="PLB01_09990"
     CDS             7657..9351
                     /locus_tag="PLB01_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQQ77673.1"
                     /translation="MGPRRWAPGLAAARDYEPAWFRRDLLAGLVLSTVLVPVGMGYAQ
                     ASGLPAIYGLYATIVPLLAYAVFGPSRVMVLGPDSALAALVAAAIVPLAAGSVDRAVA
                     LAGMLAILTGALCALGGFFRLGFVTDLLSKPIRYGYLNGIALTVLVGQLPKVLGFPVS
                     GQSFLGEAGGLVRGLRAGEANVAACAIGLSSLIVILGFRRWAPNVPGALVAVAGATLA
                     ASQFDLARTAGLSVVGPLPQGLPAFRIPVVSAADVVSLAAAAFAIALVSLADMSVLSR
                     MFAARNGYPSDTDQELVALGAANLATGLFQGFPVASSASRTPVAEAAGGKTQLTGVVA
                     ALSVALLLIFAPALLRDLPQAALGAVVIAACLGLVEVSGVRRLYRLRPGEFAVSAVCF
                     FGVVLVGVTQGIFIAVGVALLAFVWRAWRPYDAVLGLVDGRKGYHDVSRHPEAARIPG
                     LVLFRWDAPLFFANAETFREHVLRAVAEAPTPTAWVVVAAEPVTDVDITAADALAGLD
                     LALHGAGIELCFAEMKGPVKDRLKRYGLFTQLGTENFFPTLEQAVEAYCAKFRIEV"
     gene            9355..11052
                     /locus_tag="PLB01_09995"
     CDS             9355..11052
                     /locus_tag="PLB01_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQQ77674.1"
                     /translation="MLLLNPRDLSAFGLDAPSRALLEDTVAFFEKKGKKKLKDDDRER
                     VWYRDFLDFQKEKKLFATFLTPAPLGSGAARWDTFRNCALNEVLGFYGLAYWYTWQVT
                     ILGLGPFWMSGNEAMRKKAAALLSDGGIFGFGLSEKEHGADIYGTETALAPATGGGWV
                     ANGRKYYIGNGNEAALLSVFGKVAGTEDFVFFAADPNHPAYELVRNVCNSQSYVAELA
                     LHDMPIADEAVLARGPAAWDASLNTVNVGKYNLGWASIGIATHALYEAVRHAARRRLY
                     KMSVTDFPHVKRMFTDAWARLVAMRLFALRASDYFRTASKEDRRYLLYNPVVKMKVTT
                     QGEAVVDLLWDVIAAKGFERDMAFEMAARDIRALPKLEGTVHVNIALILKFMPNYFFA
                     PAPYPDVPRRTDAADDAFLFDQGPAHGLSKIRFHDFRPAFAAWDLPNVNLFEEQIAAL
                     RGFLAKRSVTEAQQKDLDFLLTLGELFTLVVYGQLVLENATLLGVAPDVVDQIFDFMV
                     RDFSRFAVELHGRAGTTPEQAADCLTMVRKPAADPARAAAIWACEVLPLADSYEMAP"
     gene            complement(11365..>12143)
                     /locus_tag="PLB01_10000"
     CDS             complement(11365..>12143)
                     /locus_tag="PLB01_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77675.1"
                     /translation="ARADFAALGGDPFSGALCDYFEASVLCFQGAFGHAERLFKAAAR
                     VFADFAQNHWVGRAEAMLAQMLVQRGNPARALASFDAAVERLDPDRDANAFTACLMNR
                     SRCLAMLGRFAAARQGYAQALQSARRHRLDALVFNVRQNLAEVELLRGDPGKALASYR
                     AVAAEADRLGLEEDAVVTRLAAAECLGRLGRADEMTEALREIGRLVAVTDLAGNPAWT
                     ELAARLDPGDIDVGLVSEVRRHLEAASDGFVLPFRAARRA"
BASE COUNT         1621 a         4313 c         4283 g         1926 t
ORIGIN      
        1 tcgcgtggcc cgcgcgcgag gcggtgctcg cgtacgccgg ccgcgcggac gccgccgtcg
       61 aagacgcgct ccggtccggc gcgtgcgacg cgcacgcgga cgccttctcc accgtggtcg
      121 agcacgagct catgcaccag gagacgctgc ggtacatgct ccaccggctg ccgtacgcgg
      181 agaagcgcgc tcccgcgggg gcgccggctc ccgaaacggc gggcccgagc gtgcacgcgg
      241 atgcggtgct cgtccccgcg ggcgtcgcga cgctcggcgc ggttccgggc gggattccat
      301 tcgggtggga caacgagttt ccgaaggcct gccggttcgt ccccgccttc acggtcgacg
      361 cgctcccggt gacgaacggg gacttcctgg ccttcgtcga ggcggggggc tacgcggacg
      421 ccgacgtctg ggcgcccggc gacgccgagt ggctcgcgtc gcacgggatc acgcatccgc
      481 cgttctggcg gcgggagggc ggcgcctggt tctgggtcgg gcagttcggc ctcgtcccgc
      541 tgcctcccgc gtggcccgtc tgggtgagcc acgccgaggc ctcggcctac gcacgctgga
      601 agggccggag gctcccgacc gaagccgaat accaccgcgc cgcgttcgga acccccgaag
      661 ggtccgagcg gtcgttcccc tggggcgacg cggcgcccgc gccggagcac gggaacttcg
      721 gcggccaccg cgacgacccc gtgcccgcgg gctcccggcc ggcaggcgcg agcgcgtggg
      781 gcgtgcacga gctcttcggg aacggctggg agtggacctc gacaccgttc gcgccgttcg
      841 agggcttcat gccgatggcg acgtatcccc gttactccgc ggacttcttc gacggccgcc
      901 actacgtcct caagggagcg tctcccgcga cgccggcgcc gctcgtgagg cggacctttc
      961 gcaattggtt ccaggcgcgt tacccccacg tcttcgcgaa gttccggtgc gtgcacccgt
     1021 gagcctcgcc gccgacgtga aggccgggtt cgcgaggacg ccgcgctccc tgccgccgaa
     1081 gtacttctac gacgagctcg gatcccgcct cttcgaagcc atctgccggc tgccgtggta
     1141 tcggatcacg gcggccgaga cgcggctcct ccggcgggtg gcgccggcgg tcgcgcgggc
     1201 gctcgcgggg cccgtccgcg tggtcgagct gggctcgggg agcggcgaga agctcgcggt
     1261 cgtcgtggag gccctccgct ccgggcggaa gcggccggac gtccacgtcg tcgacctctc
     1321 ggccgcggcg gtgtccgcgt cgctcgcgcg cctcgcgaag atcccgggcg tgccggcgaa
     1381 gggccaccgc gcggcgtacg agaccggcct cgcggacgcg acggcagcgc ggcgcggggc
     1441 gggcgcgtgt ctcgtcctct tcctcggctc gaacatcggg aacttcgacc ccccggacgc
     1501 ggcggggctg cttgccgcgg tgcgccgccg cctgcgcccc ggcgacgcaa tcctcctggg
     1561 gaccgacctc gtgaagcccg cgcacgagct cctcgcggcc tacgacgacc cgctcggtgt
     1621 gacggcggcg ttcaaccgga acgtcctcgt gcgcctgaac cgcgagctcg gcgccgactt
     1681 cgacgtcccg gcgtggtgcc accgcgccgt ctggaacggg cgggcgcgcc gcgtcgagat
     1741 gcacctcgtg agcctcgtgc gccagcgcgt cacgatcccg ctggccgccg ttgccgccgt
     1801 gttcgaggcc ggcgagacga tccacacgga gagctcgcac aagtaccggc ctgccgacgt
     1861 cgaccggctc gccctggccg ccgggttccg ccgcgagcgc cgctggatcg accggtccgc
     1921 acggttcgcc ctgactctct gccgcgttga ggaggagccc tgaagctgcc cggagcctgt
     1981 gctcacatcg ccgcgctgga ggccgtggtt cccgcgaagg ccgcggagtg cgaggagtgc
     2041 gtcaagatcg gatcggagtg ggtccacctc cggacgtgcc aggcgtgcgg cggcacgcgc
     2101 tgctgcgatt cctccccgaa ccgtcatgct tcgaagcacg cgaagagctc gggccacccc
     2161 gtcatcgcct cggcggagcc cggcgagcgc tggctttact gctatcccga cgacgccttc
     2221 gtggagttct gaagcctcgg gacgattgag aggcgcctag aatggacggg ccatgaaggg
     2281 gagcctcccg gcgctcgtcc tcctcgcagc ggtcgcggcg gtcggcgcgc agtccctgaa
     2341 tcccggcggg aagctcgtcg agacgaaaga cggaatcgtc tttcagagga acacgccggc
     2401 cccgcggcct acccgggagc gcgcggatca gcccgccgac accggtggcg gcctcgtgaa
     2461 ggtcgcgacc ccgccgggcc ccacggggcc ggccgtcgtg gacgcggcct acgtcacgtt
     2521 ccgcggcacg gtcgtgcggt tcgagaaagg cgccgcgatc accattctcg acggccggac
     2581 ggggaaggag cggcgcgtct ttctcgccaa ggccgccgtc gtccccgatg gaatcaaggc
     2641 gggcgacgcg gtcgccgtgc gcgtccccct cgaagagggc tccggcgccc gcaccgcaga
     2701 ccgcgtggag ctccagaaga cgcccccgcc ggtggacccg aagtcgaagt tcactcaggc
     2761 gaaggcgccc gcaacccgct gactcgcgat cagccgcgcg gaggagagtc gaggcggcag
     2821 gctttcgaca cgcggtaccg gttctcggcg aaggcccggt aacaaaaatc gaacaggcgc
     2881 cgaaagcccg gcgtggcgga gagcagccac agcggcgtcg cccaccagat gcggcggaac
     2941 gcccagcggt acgcgtccgc tcccgccatc acgtctcccg aacccgccac gaggacgcgc
     3001 aggtcgtcga ggaggttctc cgtggccaca cccttcgcct cgagccgttc gacgatccac
     3061 ggctcctgga gcgtcgcgat cccgatgccg cgtcttgcga gcgtgccctt ccagaacggg
     3121 acccaccggc tgcagaaccc gcacgcgccg tcatagagga cccaggcgag gggcggcgtc
     3181 acggtttcca gcgcagggcc gcgaggtcga gacggccgct gcggtcgaag cgcaccttct
     3241 ccttctcgag gaggacgcgc tgcaggacgt ggtgctcgga gccgtcgcgg cgcgggctca
     3301 cctcgccctt cgcgttcacg acgcgatgcc acgggacggc gctgcgcggc ggaagcgcgt
     3361 ggagcgcgta gcccgcgagc cgcgcatggt tcggaaggcc cgcgagccgc gcaacctgcc
     3421 cgtacgtcgc gacgcgtccg ggcgggatcc tcgccgcgac ggcccagatc ctccggtaga
     3481 gggaatccgc gcgcgcggcc acgctagtcg tcctccagcc ggctccaggc cgccgaggcg
     3541 atccgccagc cgcccttttc ggattcgagc cgcacgtcga agctccagcg cgaggaccgc
     3601 gggaggagtc cctcgagtcc gcccacggcg cgcggccggc ccgacatccg caccttgaac
     3661 ttcgcctgcg cggccgtggc gccgcgctcg accgcgaggt ccgagagcgt gagggagagg
     3721 gactcgtacg cggcgagagt gcgccggacg agcgcggcgg cgtcggcctt tccgccgccg
     3781 tccgcgcccc ggtaactctc ggcgacgcgg gccatcacgg cgtccgcgtc gcgtttctcc
     3841 gcggaggcga ccattcgttc caggcagtcc ctcacgggat ccgccgggcg cccgcaagag
     3901 aggagcaggg cggcgaccgc gggaaccaag gcccgcgccg ggagcttcat ggggcgatcg
     3961 tacgcgcccg cccggcgccg agggctagcg ttccttcttc gaccgggtct tccccttcga
     4021 aggagtcttc tcctcttcga ctttcacgtc tccgatcacg gtcttgacgg ggcagaacga
     4081 gaagatcggg cacttcctgc aaccgacggc gagggcgacg ggacagagaa ccatgtggga
     4141 ctcctcgggc gcgatgcgca cccgcaccca gcctacaccc ggcccgccgc ccgcgtggac
     4201 ggcctacttc ttcgcggcct tcgtgcaggt ttcctcgtcc gtgttcaccc acttgccgtc
     4261 gggaccctgc attgcgcccg cgtggacgac ctcggtggcg ctcttcctcg tgaagacgtc
     4321 cctcacggtc atcttctttc cgtccatgtt cccgggacct tcgaacgtca ggacgtcgcc
     4381 ggcccagccc ttcgacgtcg cgttgcacca gccgccgaag ctgtcgtggc agcgctggat
     4441 gaagactttc tctcccgtgt cgtagcccat gaagcccgcg gcgtggtacg gcatggggtt
     4501 ggcggccgtc ttcgcctcgt cgtagtggaa ggcgagccag tagccgccga gggcgctcgc
     4561 gaatccgacc ttcgcctcca cggcgtgctc gggacccatc ggggtcgcga acgtcttgcc
     4621 ggtgcacgtc cagttgccga ggaagtacga cagctgcgaa agctcctttg gcggcttggg
     4681 agccgcggcg ggcttgtcct ccgcgagggc gagaggagcg gcgacggcga gcagggcggc
     4741 gaaaacggcg gcacggtgag acggcaggcg catgggggtt ctcctccttt tcgttccgcg
     4801 gagtctagcg ccgccgagac ggcgtctcaa cgggccctct tcaccgggcg agccagaccg
     4861 ccgcgagcgc gagggcgccc ggcaacgcct gcacgtagag aatcgtcttc ttcgccgttg
     4921 ccgctccgaa gactcccgcg acgacgacgc agacgaggaa gaagaccttc acggcgaagc
     4981 cggcggggcc gaggagcagg ccccagacga ggcccgcggc gaggaagccg ttgtagaggc
     5041 cctggttggc cgcgagcgga gcggaggccg ccatgacgtc cggcggcagg ccgaagacgc
     5101 ggcggcccgt cggcgtcttc cacaggaaca tctcgaggac gaggaaaccc gcgtggagaa
     5161 gcgcgacgag gccgacgagg acgaccgcga gagtgtgcat ggaggcggac gatatcccgg
     5221 cggcaggtgg ttggccgacg aaggccttaa actcgacctg atccgcgatg ggcgatccgc
     5281 ttctcctcca ccgcctccag ttcgccttca cgatcctgtt tcactatctc ttcccggtcc
     5341 tcacgatggg cctcgctctc ctgatcgtcg tcatgaaggc gctcgggttg cggaaggggg
     5401 gcgagcgctg gaacgatgcg gcgcggttct ggatccgcat cttcgggatc aacttcgcga
     5461 tcggcgtcgt gacgggcatt ccgatggagt tccagttcgg gacgaactgg gcccgcttct
     5521 cgcgctacgc cggcaacgtg atcggccaca cgctcgcgat ggagggcgtc ttcgccttct
     5581 tcctcgagtc cgcgtttctc gcgctcctcg tctacgggga gaagcgcctc tcgcgggtcg
     5641 ggcacttcct ggcggccgtc gcgctcttcg tcgggagctg ggtctcgggc tggttcatca
     5701 ccgcgacgaa cgccttcatg cagcacccgg tcggccaccg catcgccgag gacgggacgc
     5761 tccggctcga gagcttctgg gcgtttctcc tgaacccgtg ggcgttcgcg gaatacgtcc
     5821 acaacatgac ggcgagcgtc gtgacggcct cgttcgtggt cgcgtcgctc ggcgcgttct
     5881 acgtcctgca gggccgccac ctggaccagg cgaagctctt cctcaagctc gggacgaccg
     5941 ccgggctcgt ttccagcgtc ctcctcgcgt ttccgacggg agacgttcag gcgaagctcg
     6001 tcgcgcggca ccagcccgtc gccctcgccg cgatggaggg gcgtttcgaa agcgggccgc
     6061 gagcgggtct gaacatgatc ggccagccga acgtgaagga gcgacgcctc gacaatcccg
     6121 tcacggtgcc gatcgttctg tccgtcctcg ctttcggcca cgtgacgagc gaggtccagg
     6181 gcctgaatga ctttccggtc tccgagtggc cggacaacgt cgagctcctc tactactcgt
     6241 ttcacgtcat ggcgggcctc gggacgctcc tgatcctcgt catggcgctc gcgaagtttc
     6301 tcgcgtggcg gggacgcctc gagacgtcct cgccgatgct ctgggtgctc atgctcgcgt
     6361 tcccgttccc gtacattgcg gtgctcgcgg gctggctcac gaccgagctc gggcgccagc
     6421 cgtggctcgt gtacgggctc ctgagaacgg cgcaggggtc gagccccacg gtccacgccg
     6481 gcacgacgct cttcacactc ctcgggttct gcgggatcta cacggtcctc gccttcctgt
     6541 tcgccttcct cgtgtcgctg gaaatcgcgc gcgggcccgg gggccgccct gcggcggaga
     6601 cgtcccatgc atgaggtgtg gtacgggacc gtcgtcttca tgctcacggc gtatgtcgtg
     6661 ctcgacggtt tcgacctggg cgccggcgcg ctgcatctct tcgtcgcgcg aagcgacgcc
     6721 gagcgccgcc aggtgctcgc ggcaatcggc ccgttctggg acgggaacga ggtctggctc
     6781 ctcgcggccg gcggcgctct cttcgtcggg ttcccccgcg tcctctcctc ggggatctcg
     6841 ggcttctact tcgcgatctt cctcgtcctc tggtgcctga tcctccgcgg gatctcgatc
     6901 gagcttcgca gccacgtgaa ggacatggtc tggcggaaga cgtgggacgc gggcttcgcg
     6961 gtcgcgagcg tcctgctgcc cgtcttcttc ggcgccgcgc tcggcaacct cattcggggc
     7021 gtgccgctcg acgccgaggg ctggttcgaa ctcacgctct tcaccgactt cacgcctgcg
     7081 ccgcccgccg gaatcctcga ctggtacacg gtggccgtgg gtgttttcgc gctggccgcc
     7141 ctcgctgctc acggggggac gtttctggcg tggaagacgg acggcgccgt gcagagccgg
     7201 agccgccgcg ctgcggtcgc cctctactcc gcagtggccg tgctctgggc cgccgtgacg
     7261 ggtgcgacgt cctccgtgaa tccggacctt ctcgccgcgc tgccgcgccg tccgctcgcg
     7321 ctcttgctcg ccgtcgtcgc ctttgccggt cttgccggcg tcttcgtcgc gcacgcgaga
     7381 gggcacgatc gagcggcctt cgtctcctcg agcgccttcg tcgcggggct ttcggtcgca
     7441 acggccgtat gcgtgtggcc cgtcctgctc cgcgccgagc cggacggagc gcgctccctc
     7501 acggccctca acgcgggcgg cgatccggcg gggttgagaa cggggctcgc gtggctcgcg
     7561 atcggccttc ccctcgtcgc ggcgtactac gtctttctct tccggttcca ccgcggaaag
     7621 gtggccgcag cggctgaggg cgaggggtac tgagccgtgg gcccgcggcg ctgggccccc
     7681 ggtctcgcgg cggcccgcga ctacgagccg gcctggttcc ggagggacct cctcgcgggg
     7741 ctcgtgctct cgacggttct cgtcccggtc gggatggggt acgcgcaggc ctcggggctg
     7801 cccgccatct acggtctcta tgcgacgatc gtcccgctcc tcgcctacgc cgttttcggc
     7861 ccgagccggg tcatggtcct cgggccggat tcggccctcg cggccctcgt cgcggcggcg
     7921 atcgtgccgc tcgccgcggg aagcgtcgac cgggccgtcg cgctcgcggg gatgctcgcg
     7981 atcctgacgg gcgccctgtg cgccctcggc gggttcttcc ggttgggctt cgtgacggac
     8041 ctcctctcga agcccatccg ctacggctac ctcaacggga tcgcgctcac cgtcctcgtc
     8101 ggccagctcc cgaaggtgct cggttttccg gtgagcgggc agagcttcct cggggaggcg
     8161 ggcggcctcg tgcggggcct gcgcgccggc gaggcgaatg tggcggcgtg cgcgatcggc
     8221 ctctcgagcc tgatcgtgat cctcgggttc aggcgctggg cgccgaacgt cccgggcgcc
     8281 ctcgtcgcgg tcgccggcgc gacgctggcc gcctcacagt tcgatctcgc ccggacggcg
     8341 ggcctttccg tcgtcgggcc tctcccgcag ggtctgcccg ccttccggat cccggtcgtt
     8401 tccgcggccg acgtcgtctc gctcgctgcg gcggcattcg cgatcgccct cgtctcgctc
     8461 gcggacatga gcgtcctgtc gcggatgttc gcggcgcgga acggctaccc gtccgacacg
     8521 gaccaggagc tcgtcgctct gggagcggcg aatctcgcga cgggcctgtt ccaggggttc
     8581 ccggtcgcga gcagcgcgtc gcgcacgccg gtcgccgagg cggccggggg aaagacccag
     8641 ctcaccggag tcgtcgccgc gctctccgtc gcgctcctcc tgatcttcgc gccggcgctg
     8701 ctccgggacc tcccccaggc ggcgctcgga gccgtcgtga tcgcggcgtg cctcggcctc
     8761 gtggaggtct ccggcgtgcg gcggctgtac cggctcaggc cgggggagtt cgcggtctcg
     8821 gccgtgtgct tcttcggcgt cgtgctcgtg ggcgtcacgc agggcatctt catcgccgtc
     8881 ggcgtcgcgc ttctcgcctt cgtctggcgc gcgtggaggc cctacgacgc ggtgctcggc
     8941 ctcgtcgacg gacggaaggg ctaccacgac gtctcgcggc acccggaggc cgcgcgcatc
     9001 cccggcctcg tcctcttccg ctgggacgcc cccctgttct tcgcgaacgc cgagacgttc
     9061 cgcgagcacg tcctccgcgc cgtcgccgag gcgccgacgc cgacggcgtg ggtcgtcgtc
     9121 gccgccgagc ccgtcacgga cgtcgacatc accgccgccg acgccctcgc cgggctcgac
     9181 ctggcgctgc acggggcggg gatcgagctg tgcttcgcgg agatgaaagg ccccgtgaag
     9241 gaccgcctga agcggtacgg cctcttcacc cagctcggga cggagaactt cttcccgacg
     9301 ctcgagcagg ccgtcgaagc gtactgtgcg aaattccgaa tcgaggtgtg accgatgctc
     9361 ctcctgaacc cccgggacct ctccgccttc ggcctcgacg cgccctcgcg cgcgctcctc
     9421 gaggacaccg tcgcgttctt cgagaagaag ggaaagaaga agctcaagga cgacgaccgc
     9481 gaacgcgtct ggtaccgcga cttcctcgac ttccagaagg aaaagaagct cttcgcgaca
     9541 ttcctgacgc cggcgccgct cggctcgggc gcggcgcgct gggacacgtt ccggaactgc
     9601 gccctcaacg aggtcctcgg cttttacggc ctcgcgtact ggtacacgtg gcaggtcacg
     9661 atcctcggcc tcggaccgtt ctggatgagc gggaacgagg cgatgcggaa gaaagccgcg
     9721 gccctcctct cggacggcgg gatcttcggg ttcggcctct cggagaagga gcacggcgcg
     9781 gacatctacg gcacggagac ggcgctcgcg ccggcgaccg gcggcggatg ggtcgcgaac
     9841 ggccggaagt actacatcgg gaacggcaac gaggcggcgc tgctgtccgt cttcgggaag
     9901 gtcgcgggca cggaggactt cgtcttcttc gcggcggacc cgaatcaccc ggcgtacgag
     9961 ctcgtccgca acgtatgcaa cagccagtcg tacgtcgccg agctcgcgct gcacgacatg
    10021 ccgattgcgg acgaggccgt cctcgcgcga gggcccgcgg cgtgggacgc gtcgctcaac
    10081 acggtgaacg tcgggaagta caacctcgga tgggcgtcga tcgggatcgc gacgcacgcg
    10141 ctctacgagg ccgtccggca cgcggcacgg cggcggctct acaagatgag cgtcacggac
    10201 tttccgcacg tgaagcggat gttcacggac gcgtgggcgc gcctcgtcgc gatgcgcctc
    10261 ttcgcgctcc gggcgtcgga ctacttccgg accgcgtcga aggaggaccg gcgctacctc
    10321 ctctacaacc cggtcgtgaa gatgaaggtc acgacgcagg gtgaggctgt cgtcgatctc
    10381 ctgtgggacg tcatcgcggc gaagggcttc gagcgcgaca tggcgttcga gatggccgcg
    10441 cgcgacatcc gggcgctccc gaagctcgag ggcaccgtcc acgtgaacat cgcgctgatc
    10501 ctgaagttca tgccgaacta cttcttcgcg ccggcgccgt atccggacgt cccgcgccgg
    10561 acggacgcgg ccgacgacgc tttcctcttc gaccagggcc cggcgcacgg gctctcgaag
    10621 atccgattcc acgacttccg ccccgccttc gcggcgtggg acctgccgaa cgtgaacctc
    10681 ttcgaggagc agatcgcggc gctccgcggc ttcctcgcga agcgcagcgt gacggaggcc
    10741 cagcagaagg acctcgactt cctcctgacg ctcggagagc tcttcacgct cgtcgtctac
    10801 gggcagctcg ttctcgagaa cgcgacgctc ctcggcgtgg cgccggacgt cgtggaccag
    10861 atcttcgact tcatggtgcg ggacttctcg cgcttcgcgg tcgagctgca cggccgcgcc
    10921 gggacgacgc cggagcaggc ggccgactgc ctgacgatgg tgcggaagcc cgcggccgat
    10981 cccgcccgcg ccgccgcgat ctgggcgtgc gaggtcctgc cgctcgcgga ctcgtacgag
    11041 atggcgccgt aggaccgggc gggcgtgtcc gcccggtccc acggacctgc ggttcggttc
    11101 agcctccgat cggcgttccg ccccggccgg ggtcagtggg atcgcccgag tccggggtgg
    11161 tcgtgccgag tccgagaagc agcagaagca gggtgagagc gtccatgtct tcctccttgg
    11221 gcgcgccggt gtccggcacg cgtgtggagg cggtcgacgt gagcgctcag ctttccgcct
    11281 gttacaccct gatagacagc tggcgggggc aagattcaac cgccgcgagg gcgcttcgtg
    11341 aacgagtgcg cctgatgaag gcgcttacgc gcgacgggcg gcgcggaacg ggaggacgaa
    11401 cccgtcgctc gcggcctcga gatgccggcg cacctccgaa acgagaccga cgtcgatgtc
    11461 gcccgggtcg aggcgtgcgg cgagctccgt ccaggcggga ttgcccgcca gatcggtgac
    11521 ggccacgagc cgcccgatct cacggagcgc ctccgtcatc tcgtccgccc gcccgaggcg
    11581 gccgaggcac tccgccgctg cgaggcgggt gacgacggcg tcctcctcga ggccgaggcg
    11641 atcggcctcg gcggcgacgg cgcggtagga cgcgagggct tttccgggat cgccgcgaag
    11701 gagctcgacc tcggcgaggt tctgtctcac gttgaagacg agcgcatcca gccggtggcg
    11761 tctggcggat tgaagcgcct gagcgtagcc ctgccgcgcg gcggcgaacc gtccgagcat
    11821 ggcgaggcag cgcgatcgat tcatgagaca ggccgtgaac gcgttcgcgt cgcggtcggg
    11881 gtcgaggcgc tcgacggcgg cgtcgaagct cgcgagggcg cgggcggggt tgcccctctg
    11941 gacgagcatc tgggcgagca tggcctcggc gcggcccacc cagtggttct gagcgaagtc
    12001 cgcgaacacg cgggccgccg ccttgaagag ccgctcggcg tgcccgaacg cgccctggaa
    12061 acagagaacg ctcgcctcga agtagtcgca gagagcgccg ctgaaaggat cgccgccgag
    12121 cgccgcgaaa tcggcgcgcg cgg
//
LOCUS       DAOMFY010000102        12140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1622_length_12140_cov_7.957633, whole genome shotgun sequence.
ACCESSION   DAOMFY010000102 DAOMFY010000000
VERSION     DAOMFY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12140
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_12140_cov_7.957633"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLB01_10005"
     CDS             <1..529
                     /locus_tag="PLB01_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQQ77676.1"
                     /translation="ERLRVLTPDDVRAAAAKYLPKDRRVALTVVPRPPVAPAAPPPAP
                     APAPVVAPAPAPAETAVVTAPAARPATSTTGEILFDPAKSRLTNIAKAILDGVALRLK
                     NNLAATCTVSAWTDAKEKGDRAALAKARAEAAKDYLMKRHGIDGSRIATEVKGDGDAG
                     DATRNRRAVVSVTFP"
     gene            649..1737
                     /locus_tag="PLB01_10010"
     CDS             649..1737
                     /locus_tag="PLB01_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQQ77677.1"
                     /translation="MFRSRALVVLATAAVVGLLWAGKVLFVTFFFALFLSFALQPFVG
                     LLERLRLPRTLAVLAVILLLTALVVFFVVNALGQIEAFSRDLPSYETKIRAFTSKAQG
                     LFAQLEERTRSLLPEGDRTTRSVRLEADAFDSLSRVALQVKTVLSLLLYAAAVPMLSF
                     FMLKDREKYGGAVAKILRLKGGRAAQDFAEGVARVLSGYVLGEVFVVLITACVTTAGL
                     LVLRVPYGYVLGPLAGVCVLIPYVGVIMSTTPAFLVAILASSDAALPVKVLVFYSVVQ
                     FLEGNVLTPFIVGSRVRLHPLAVLFAFLFWGILWGVPGAILAVPLTATIKVVTERFER
                     YAPWAALLGERTPDPEPPEEPESLLANL"
     gene            complement(1759..2664)
                     /locus_tag="PLB01_10015"
     CDS             complement(1759..2664)
                     /locus_tag="PLB01_10015"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HQQ77678.1"
                     /translation="MKGAVSLGALWSETAESLVRARRMTSLSVALIAISIGLVGAFFL
                     VAENLRGVVETVRDESAVTVFLRPGVTDADRADLERVARDSRLVAKTKRVTPEQARAR
                     FSDWWRSLAPAARALPTNPFPESLELELEPSAVTSEAIPGFLKNLAAHRAVEEVQFDV
                     DWIRRLRGAVQLAKIAGFVLAALLTLGAAFTIANVVRLTILLHRDEIEILRLVGAPEL
                     LIRGPFVLGGLAQGLLGAALAVAGLWLVYRGVIAWVAATKNAFLGVFAVRFLPGSTSL
                     LLLAFGVAAGVAGGLLAVRRKNLAG"
     gene            complement(2661..3371)
                     /gene="ftsE"
                     /locus_tag="PLB01_10020"
     CDS             complement(2661..3371)
                     /gene="ftsE"
                     /locus_tag="PLB01_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HQQ77679.1"
                     /translation="MIEFYRVAKEYQGRRVLKDVTFQAKKGEFLFLTGPSGAGKTTLL
                     RLIFRAEAPTEGQIIVNGMNVSGMPPSRVPSLRRSMGVVFQDYKLLPRRTVLENVTYV
                     QKFLGVPAAERRRRAYQTLRLVELHHRMSALPEELSGGEQQRVALARALVNDPVLLVA
                     DEPTGNLDHRLAEEVMKLLVEISLRGTTVLVATHDQDLIRETGRRCLTLDGGRVVEGL
                     VEGALPPQAAHRREEAPR"
     gene            complement(3368..4831)
                     /gene="gatB"
                     /locus_tag="PLB01_10025"
     CDS             complement(3368..4831)
                     /gene="gatB"
                     /locus_tag="PLB01_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQQ77680.1"
                     /translation="MPTSPFEPVIGLEVHAQLKTKSKTFCACPVTFGAPPNTAVCPVC
                     LGYPGTLPVPNREMVTLAMRVALAAGCTIQARSVFERKNYFYPDLPKGYQISQYERPL
                     ATGGRVAVETDAGRREIRLNRIHLEEDAGKSMHEVPWDDVSSSASLVDLNRAGTPLIE
                     IVTEPEIASPEEAFDYLLRLRRLVRWVDASDGNMEEGSLRCDANVSLRPRGAGALGVK
                     VEIKNLNSIAHVKKALEHEIARQASLLSAGGTVVQETRLFEPGTGTTKTMRTKEEAMD
                     YRYFPDPDLGALEVDAAWVEAVRASLPEMPEPRAERLATQYALPAADAELLCSSRPLA
                     DWYEAAAALHPSNPKAVANWLLSDLLGRMSDADRQAGRVPVSPGHLAALVALIDAGTI
                     SGKIAKEILPDVIETGKAPVDLVKEKGLVQISDEGALGEAVAAVIAANPEQVAAYRGG
                     KAATFGWFVGQVMKATGGKAAPAVVNRLLKERLGPPA"
     gene            complement(4870..6102)
                     /locus_tag="PLB01_10030"
     CDS             complement(4870..6102)
                     /locus_tag="PLB01_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ77681.1"
                     /translation="MSAAVPAPAADPPGRWRVLVLLAVAEFLGMSLWFSASAVSPLLR
                     AEWNLSDSAATWLTLSVQLGFVAGTLASAILNLPDVWAPRQLVVGASLLGAAANACLA
                     LYSHGPVSALVLRFLTGFWLAGVYPPGMKILATWFKARRGLALGVLIGALTLGKGVPY
                     LANAIFGSAWRSTLLATSGGAVLGALLVFLFVREGPFAPAPARFDPRQVTKVFANRGV
                     RLASFGYFGHMWELYAMWTWIPVMLRSSFALRGLPHAAAEAASFLVIGCGAAGCVAAG
                     LAADRKGRTLVTSAAMAVSGACCVAIGFCYGGPPLVLLAVAAVWGFSVVADSAQFSAA
                     VTELGDVAYMGTALTLQTSIGFFLTAISIDLVPTFLPALSWKWVFVLLAPGPALGVLA
                     MLRLRGLPEAVKIAQGRR"
     gene            complement(6099..7502)
                     /locus_tag="PLB01_10035"
     CDS             complement(6099..7502)
                     /locus_tag="PLB01_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQQ77682.1"
                     /translation="MARREISPEEARSLAAIVGETHLLAAADEVAPYAKDWTRDLVAV
                     PAAVARPRTTEEVSRILAFCNARAIPVTPQGGRTGMSGGALAVFGGLGLALDRMTSIL
                     EIDADNFVAVAEPGVVTQALQEAVEGRGLYYPPDPASRGSCTIGGNVAENAGGPHAAK
                     YGVTGRWVMGLEAVFADGSIAATGGKTRKDVAGYDLTSLLVGSEGTLAVVTKAWLRLI
                     AKPAAWTTVLAPFPSLESAAQAVFEIHRRGLVPSACEVVDRPAVEVASRKKGIPVPFP
                     EAEARLLLDFDAATEEEVEREVTAAGEILIECAALDVAVAADEPRRRALWDVRRGIAE
                     AIHELGVSFELDLAVPRSELVALVTGVRRRAAEAHLRAFTYGHAADGNLHVRLLREGS
                     ATFDEAAVEAVMAAVYEETVRLGGTITGEHGVGLTLRKFLPLRRPPAFLAAMRSVKHA
                     LDPNGILNPGKLVPDAA"
     gene            7525..8385
                     /locus_tag="PLB01_10040"
     CDS             7525..8385
                     /locus_tag="PLB01_10040"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ77683.1"
                     /translation="MTRVAFLGLGAMGAPMARRIVAAGLPTTVWNRNPGRAAPFAALG
                     ASVAKTPREAAAAADVVCTMLSDPAAVEAVASGPDGLIAGLAPGALWLDFSTVTPAAS
                     RSFDARAREKGARFCDVPVAGSVKPATDGTLKILAGGDPAELRKATPVLEAVAKGVLH
                     FGAVGQGSAMKLVNNLLFGVTLAAFGEALGLARRLGLPEKETTEWLLSIPSVSPYIKA
                     KLDHLAAGGEPPAFQLSLMEKDLRLMVEAGGGPEKAPVVEAARADYERARRAGLGEKD
                     FSHVLSFLKS"
     gene            complement(8382..9116)
                     /locus_tag="PLB01_10045"
     CDS             complement(8382..9116)
                     /locus_tag="PLB01_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HQQ77684.1"
                     /translation="MSARLAAPAGRRGTRTCATRLLTVLRRELARAGSPERAAGARAY
                     MKSAMPFHGVGAVPLRAICRKAFAAHPVEDPESWRKDVLALWRGARFREERYAAIELT
                     GDRRARPFQTMASLPMYEEMIVTGAWWDLVDALATKRLGEILRNEPAPMRKAMRAWSR
                     GPDMWKRRSAILCQVTFKKDTDLDLLFGLIEPSIGSKEFFLRKAIGWALRSYAWTDER
                     PIVTYVEKNRARLSGLSVREALKNVR"
     gene            complement(9113..9691)
                     /locus_tag="PLB01_10050"
     CDS             complement(9113..9691)
                     /locus_tag="PLB01_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQQ77685.1"
                     /translation="MTHPTKQRLLDAGLKMLLEHGYNDLGVQGLLAATGTPKGSFYHH
                     FRDKEDFALQVIDRYMQNVHAALDACLGDEGRPPLGRVRRFFELTQEHYRKEGYMGCL
                     LGGLGQELSGVNDTFRRKIEGCFSEIARRLAVCLEEARRRGDIRSGSDTRRMASVLVD
                     CWEGAALRSRLRGNAAPLDAMLDFYFRSAVGK"
     gene            complement(9729..10115)
                     /locus_tag="PLB01_10055"
     CDS             complement(9729..10115)
                     /locus_tag="PLB01_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQQ77686.1"
                     /translation="MRVAKNDVDVRMQIPGAVIRQRTDFGDASGFGSLSGECFTLAAG
                     VDTTPLFQGLEGNLCQCPHWGFVLSGQLTTTDAKGAQETVKTNDLFYWPPGHNVRVDA
                     DAEIVMFSPQREHGHVIAHMIEKVKG"
     gene            complement(10240..11262)
                     /locus_tag="PLB01_10060"
     CDS             complement(10240..11262)
                     /locus_tag="PLB01_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ77687.1"
                     /translation="MRRPVVLITGASGEMGHGLITRLAAEGTRPILTLDLKALDPSLG
                     RLVAREFTGSVLDRSVLERILSEYEIDMIFHLAALLSTRGEFTPVTAHQVNVEGMINM
                     LEFAQSERQSHGRPVTFLYPSSIAAYGLPDLAAKALAGKVKEDEFNTPTTMYGCNKLY
                     AEHLGRYYARHYKQLSSENQGGRVDFRCLRFPGLISAVTVPSGGTSDYAPEMIHAAAE
                     GKPYACFVREDTRIPFMAMPDGVDALLQLASAPAGTLTRQVYNVGAFAPSAGEIRDLV
                     LSSFPAARVTFEPDDKRQGIVDSWPADVDDSAARADWGFSPKYGLESAFSEYLIPTIR
                     ERYRRA"
     gene            complement(11262..>12140)
                     /locus_tag="PLB01_10065"
     CDS             complement(11262..>12140)
                     /locus_tag="PLB01_10065"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HQQ77688.1"
                     /translation="GRLAGTGYLSILPVDQGIEHSGAASFAPSPEYFDPENIVKLAYE
                     GGCNAVASTLGVLGSVARTWAHRIPFIVKINHNELLTLPTSYDQTIFATVKQAFDMGA
                     AGIGATIYFGSDNSRRQITEISNAFHQAHDYGMFTVLWCYLRNPQFKTKEKDFHVSAD
                     LSSQANHLGVTIEADIIKQKQPENDGGFPALPFGNKPNAEAYAKLVPAHPIEWTRWQV
                     VNCYMGRAGLINSGGAAGGKDDLGQAVATAVINKRAGGMGLISGRKAFQKPMKEGVAL
                     LNAIQDVYLETKVTVA"
BASE COUNT         1756 a         4350 c         4258 g         1776 t
ORIGIN      
        1 ggagcggctc cgcgtcctca cgccggacga cgtccgcgcc gccgcggcga agtacctgcc
       61 gaaggaccgg cgcgtggccc tcaccgtcgt gccgcgcccg ccggtcgcgc ccgccgcccc
      121 gcccccggct cccgctcccg cgcccgtcgt ggcgcccgcg ccggctccgg ccgagaccgc
      181 cgtcgtgacc gctcccgccg cgcggccggc aacctcgacg acgggcgaga tcctcttcga
      241 cccggccaag agccgcctca cgaacatcgc caaggcgatc ctcgacggcg tcgccctgcg
      301 cctgaagaac aacctcgcgg cgacgtgcac cgtctccgcc tggacggacg ccaaggaaaa
      361 gggcgaccgc gcggcccttg cgaaggcccg cgccgaggcc gcgaaggact acctcatgaa
      421 gcggcacggg atcgacggat cgcgcatcgc gaccgaggtg aagggcgacg gcgacgccgg
      481 cgacgccacc cggaaccgcc gggcggtcgt ttccgtcacc ttcccctgag ccggacctga
      541 ttcggcccgc cacggcggcg ttacactgac gccgccgtgg aatcgggatc gagcggactc
      601 ttctccaccg gaacgttttc gacgggcgcc ggccccggcg ccggcggcgt gttccggtcc
      661 cgcgcgctcg tcgtcctcgc gacggccgcc gtcgtcgggc tcctctgggc gggcaaggtc
      721 ctgttcgtga ccttcttctt cgccctgttc ctttccttcg ccctccagcc gttcgtcggg
      781 ctcctggagc ggctccgcct tccgcgcacg ctcgcggtgc tcgccgtgat cctgcttctc
      841 acggcgctcg tcgtcttttt cgtcgtgaac gccctcggcc agatcgaggc gttctcgcgc
      901 gaccttccgt cgtacgagac gaagatccgc gccttcacgt ccaaggccca ggggctcttt
      961 gcgcagctcg aggagaggac gcgctcgctc ctgcctgagg gcgaccggac gacgcggtcc
     1021 gtccgcctcg aggcggacgc cttcgactct ctttcgcgcg tcgcgctcca ggtcaagacg
     1081 gtcctgtcgc tcctgctcta cgcggccgcc gtcccgatgc tctcgttctt catgctcaag
     1141 gaccgcgaaa agtacggcgg cgccgtcgcg aagatcctcc ggctgaaggg cggacgcgcc
     1201 gcgcaggact tcgccgaggg cgtcgcccgt gtcctgtcgg gctacgtcct cggagaggtt
     1261 ttcgtcgtcc tcatcacggc gtgcgtgacg acggccggcc tcctcgtcct gcgcgtcccc
     1321 tacggttacg tgctcgggcc gctcgccggc gtgtgcgtcc tcatcccgta cgtcggcgtc
     1381 atcatgtcga cgacgcccgc gtttctcgtc gcgatcctcg cctcgagtga cgcggctctg
     1441 cccgtcaagg ttctcgtctt ctactcggtt gtccagttcc tcgagggcaa cgtcctgacg
     1501 ccgttcatcg tcgggagccg cgtgcgcctg cacccgctcg ccgtgctctt cgcgtttctc
     1561 ttctggggca tcctgtgggg cgtcccgggc gcgatcctcg cggtgccgct cacggcgacg
     1621 atcaaggtcg tcacggagcg cttcgagcgc tacgcaccct gggccgcgct cctcggcgag
     1681 cggacgccgg accccgagcc gccggaggaa cccgagtcgc tcctcgcgaa tctctgatcc
     1741 tggaaacccg tcaggcggct agcccgcgag gttcttacgg cggaccgcga ggaggccgcc
     1801 cgcgacgccg gccgcgacgc cgaacgcgag caggagcagc gacgtcgacc ccggcaggaa
     1861 ccgcacggcg aagacgccga ggaacgcgtt cttcgtcgcg gccacccacg cgatgacgcc
     1921 gcggtacacg agccagagcc ccgcgaccgc gagcgcggcg ccgaggaggc cctgcgcgag
     1981 gccgccgagg acgaacggcc cgcggatcag gagctcgggc gcgccgacga gccgcaggat
     2041 ctcgatctcg tcgcggtgga ggaggatcgt gagccgcacg acgttcgcga tcgtgaacgc
     2101 ggctcccagc gtcaggagcg ccgcgaggac gaaacccgcg atcttcgcga gctggacggc
     2161 gcccctgagg cggcggatcc agtccacgtc gaactggacc tcctcgaccg cgcggtgcgc
     2221 ggcgaggttc ttcaggaagc ccgggatcgc ctcggacgtg accgcggacg gctcgagctc
     2281 gagctccagc gactcgggga acgggttcgt cgggagcgcg cgcgcggcgg gcgcgagcga
     2341 gcgccaccag tccgagaagc gcgcacgcgc ctgctccggc gtcacgcgtt tcgtcttcgc
     2401 gacgaggcgc gagtcccgcg cgacgcgctc gaggtccgcc cggtccgcgt ccgtgacgcc
     2461 gggcctcagg aacaccgtca cggccgactc gtcccgcacc gtctcgacga cgccgcggag
     2521 gttttccgcg acgaggaaga acgcgccgac gaggccgatc gagatcgcga tgagcgcgac
     2581 cgagagcgac gtcatgcgcc gcgcccggac gaggctctcg gccgtctcgc tccacagcgc
     2641 gccgagagac acggcgccct tcaccgcggt gcctcctcgc gccggtgggc ggcctgcggc
     2701 ggaagcgccc cctccacgag cccctcgacg acgcggccgc cgtccagcgt gagacagcgc
     2761 cgccccgttt cccggatgag gtcctggtcg tgcgtcgcga cgagcaccgt cgtcccgcgc
     2821 aggctgatct cgacgaggag cttcatgacc tcctcggcga ggcggtggtc gaggttgccc
     2881 gtcggctcgt cggccacgag gagcacgggg tcgttcacga gcgcgcgggc gagcgcgacg
     2941 cgctgctgtt cgccgccgga gagctcctcg ggaagcgccg acatgcggtg gtggagctcg
     3001 acgagccgca gcgtctggta ggcccgccgc cggcgctcgg ccgccggcac gccgaggaac
     3061 ttctggacgt acgtgacgtt ctcgaggacc gtccggcgcg gcaggagctt gtagtcctgg
     3121 aacacgacgc ccatcgagcg gcggagcgac gggacgcgcg acggcggcat cccgctcacg
     3181 ttcatcccgt tcacgatgat ctggccctcc gtgggcgcct cggcgcggaa gatcagccgg
     3241 aggagcgtcg tcttgcccgc gcccgagggc cccgtcagga acaggaactc gcccttcttc
     3301 gcctgaaacg tgacgtcctt gaggacgcgg cggccctggt attccttggc gacgcggtag
     3361 aactcgatca cgcgggaggc ccgagccgtt ccttaagcag gcggttcacc accgcgggag
     3421 ccgccttgcc gcccgtcgcc ttcatgacct gcccgacgaa ccacccgaac gtcgcggcct
     3481 tcccgccgcg gtaggcggcg acctgctcag ggttcgccgc gatcaccgcc gcaacggcct
     3541 ccccgagtgc gccctcgtcc gagatctgga cgaggccctt ctccttcacg agatccacgg
     3601 gcgccttccc ggtctcgatg acgtccggca ggatctcctt cgcgatcttc ccggagatcg
     3661 tcccggcgtc gatgagcgcg acgagagccg cgaggtgacc gggcgagacc gggacgcgac
     3721 cggcctgccg atccgcgtcg gacatccggc cgaggaggtc cgacaggagc cagttcgcga
     3781 ccgccttcgg gttcgacggg tggagggcgg cggccgcctc gtaccagtcc gccagcggcc
     3841 gcgacgagca caggagctcc gcgtccgcgg ccggcagcgc gtactgcgtc gcgagccgct
     3901 ccgcgcgcgg ctcgggcatc tcgggcagcg aggcgcgcac ggcctcgacc catgccgcgt
     3961 ccacctcgag cgcgccgaga tcggggtccg ggaagtagcg gtagtccatc gcttcttcct
     4021 tcgtccgcat cgtcttcgtc gtgccggtgc ccggctcgaa gaggcgcgtc tcctgcacga
     4081 ccgtgccgcc cgcggagagc agggaggcct gccgcgcgat ctcgtgctcg agagccttct
     4141 tcacgtgagc gatcgaattg aggttcttga tctcgacctt cacgcccagg gcgcccgcgc
     4201 cccggggccg gagcgacacg ttcgcgtcgc atcgcagcga gccctcctcc atgttcccgt
     4261 ccgaggcgtc cacccagcgc acgaggcggc ggagccgcag gaggtagtcg aacgcctcct
     4321 ccggcgaggc gatctcgggc tcggtcacga tctcgatgag cggcgtcccc gcgcggttca
     4381 ggtcgacgag cgaggcggac gacgacacgt cgtcccacgg gacctcgtgc atggacttgc
     4441 ccgcgtcctc ctcgaggtgt atgcggttca gccggatctc ccggcgtccc gcgtccgtct
     4501 cgacggcgac gcggcctccg gtcgcgagcg gccgctcgta ctgcgagatc tggtagccct
     4561 tcggaaggtc cgggtagaag tagttcttgc gctcgaagac ggaccgcgcc tggatcgtgc
     4621 acccggccgc gagcgcgacg cgcatcgcga gcgtgaccat ctcgcggttc gggaccggca
     4681 gcgtgccggg gtagccgagg cagaccggac agaccgcggt gttcggcggg gctccgaacg
     4741 tcacggggca tgcgcagaac gtcttgctct tcgtcttgag ctgcgcgtgc acctcgagcc
     4801 cgatcaccgg ctcgaacggg gacgtcggca tcccgcgagt gtagcggagg agcgtgggtc
     4861 cgggccgcgt cagcgccggc cctgcgcgat cttcacggcc tccggcagcc ccctcaggcg
     4921 cagcatcgcg aggacgccga gcgcgggccc gggggcgagg aggacgaaga cccacttcca
     4981 cgagagagcc gggaggaacg tcggaacgag gtcgatcgag atggccgtca ggaagaaccc
     5041 gatcgacgtc tggagcgtca gggccgtccc catgtacgcg acgtcgccca gctccgtcac
     5101 cgctgccgaa aactgcgcgg agtccgcgac gacggagaag ccccagacgg ccgcgacggc
     5161 cagaaggacg agcggcggcc ctccgtagca gaagccgatc gcgacgcagc aggcgccgct
     5221 gacggccatc gcggcggagg tcacgagcgt ccgtcccttc cggtcggcgg cgaggcccgc
     5281 ggcgacgcag ccggccgcac cgcagccgat cacgagaaac gacgcggctt cggccgccgc
     5341 gtgcggaagg ccgcggagcg cgaagctcga ccgcagcatg acggggatcc acgtccacat
     5401 cgcgtagagc tcccacatgt ggccgaagta cccgaagctc gcgaggcgga cgccgcggtt
     5461 cgcgaagacc ttcgtgacct gcctggggtc gaaccgcgcg ggcgccggcg cgaacggccc
     5521 ttcccggacg aagagaaaca cgagcaacgc gccgaggacg gcgccgcccg acgtcgcgag
     5581 cagcgtggag cgccatgccg agccgaagat cgcatttgcg aggtacggga cgccttttcc
     5641 gagcgtgagc gcgccgatca ggacgccgag ggcgaggccg cggcgcgcct tgaaccaggt
     5701 cgcgaggatc ttcatcccgg gcgggtagac ccccgcgagc cagaagcccg tcaggaagcg
     5761 caggacgagc gcggacaccg ggccgtgtga atacagcgcc aggcacgcgt ttgccgccgc
     5821 acccaggagg ctcgcgccga cgacgagctg gcgcggcgcc cagacgtccg gcaggttcag
     5881 gatcgcgctc gcgagcgtcc ccgcgacgaa gccgagctgg acggagagcg tgagccacgt
     5941 cgccgccgag tccgacaggt tccactcggc cctcaggagc ggcgagaccg cggacgccga
     6001 gaaccagagc gacatcccga ggaactcggc gacggcgagg aggacgagaa cgcgccaccg
     6061 gcccggcggg tccgcggccg gcgccgggac ggccgcgctc acgcggcgtc cggaacgagc
     6121 ttgccggggt tcaggatccc gttgggatcg agcgcgtgct tcacggagcg catcgcggcg
     6181 aggaacgccg gcggccggcg cagcggcagg aacttccgga gcgtcaggcc gacgccgtgc
     6241 tcgcccgtga tcgtcccgcc cagccggacc gtctcctcgt agaccgcggc catgacggcc
     6301 tcgactgccg cctcgtcgaa cgtggcgctc ccctcgcgaa ggaggcgcac gtgcaggttc
     6361 ccgtcggccg cgtggccgta cgtgaaggcc cggaggtgcg cctcggcggc gcgccgcctc
     6421 acgcccgtca cgagcgcgac gagctccgag cgcggcaccg cgaggtccag ctcgaaggag
     6481 acgccgagct cgtggatcgc ctcggcgatt ccgcggcgca cgtcccagag cgcgcggcgg
     6541 cgcggctcat cggccgcgac cgcgacgtcg agcgccgcgc actcgatgag gatttccccc
     6601 gccgccgtca cctcgcgctc gacctcctcc tccgtcgcgg cgtcgaagtc gaggaggagg
     6661 cgcgcctccg cctcgggaaa cggcacggga atgcctttct tcctcgacgc gacctcgacc
     6721 gccggcctgt cgacgacctc gcacgccgac ggcacgaggc cgcggcgatg gatctcgaag
     6781 acggcctgcg ccgcggactc gagcgacggg aacggcgcga gaacggtcgt ccacgcggcc
     6841 ggcttcgcga tgaggcgcag ccacgccttc gtgacgacgg cgagcgtccc ttcgctcccg
     6901 acgaggagcg acgtgaggtc gtagcccgcg acgtccttgc gcgtcttgcc gcccgtggcc
     6961 gcgatggagc cgtcggcgaa gaccgcctcg agccccatca cccagcgccc cgtcacgccg
     7021 tacttggccg cgtgcgggcc gccggcgttc tcggccacgt tgccgccgat cgtgcacgag
     7081 ccgcgcgagg ccgggtccgg cgggtagtag aggcctcttc cctcgacggc ttcctgcagc
     7141 gcctgcgtca cgacgcccgg ctcggcgacg gcgacgaagt tgtccgcgtc gatctcgagg
     7201 atggacgtca tgcggtcgag ggcgaggccg agcccgccga agaccgcgag cgcgccgccg
     7261 gacatccccg tgcggccgcc ctgcggcgtg acggggatcg cccgcgcgtt gcagaacgcg
     7321 aggatgcgcg agacctcctc cgtcgtgcgc ggccgggcga cggcggccgg aacggcgacg
     7381 aggtcgcgcg tccagtcctt cgcgtacgga gcgacctcgt ccgcggccgc gaggaggtgc
     7441 gtctctccga cgatcgccgc gagcgagcgg gcttcctcgg gagagatctc gcgccgcgcc
     7501 accgggcgat cttaagatgg tcccatgaca cgcgtcgcct ttctcgggct cggcgccatg
     7561 ggcgccccca tggcgcggcg gatcgtcgcc gccggtctcc ccacgaccgt ttggaaccgc
     7621 aaccccgggc gcgcggcgcc gttcgcggcg ctcggcgcct ccgtcgcgaa gacaccccgc
     7681 gaggcggccg ccgcggccga cgtcgtctgc acgatgctgt cggaccccgc cgccgtcgag
     7741 gcggtcgcgt ccggccccga cggcctcatc gcgggcctcg cacccggggc gctctggctg
     7801 gacttctcga cggtgacgcc cgcggcgtcg cgttcgttcg acgcgcgcgc ccgcgaaaag
     7861 ggcgcgcgtt tctgcgacgt ccccgtcgcg ggcagcgtga agcccgcgac ggatggaacg
     7921 ctcaagatcc tcgcgggcgg cgatccggcg gagctccgga aggcgactcc ggttctcgaa
     7981 gccgtcgcga agggcgtcct gcacttcgga gccgtcgggc agggctccgc gatgaagctc
     8041 gtgaacaacc tcctcttcgg cgtcacgctc gcggcgttcg gcgaggcgct cggcctcgcg
     8101 aggcgcctcg gccttcccga aaaggagacg acggagtggc tcctctcgat cccgtccgtc
     8161 tcgccgtaca tcaaggcgaa gctggaccac cttgcggcgg gcggcgagcc gccggccttc
     8221 cagctctccc tgatggagaa ggacctgcgc ctcatggtcg aggcgggcgg cgggcccgag
     8281 aaggccccgg tcgtcgaggc cgcacgggcc gactacgagc gcgcgcgcag ggcgggactc
     8341 ggcgagaagg acttctcgca cgtcctctcg ttcctgaaga gctagcggac gttcttcagc
     8401 gcctcgcgga ccgagaggcc cgagaggcgg gcgcggttct tctcgacgta cgtgacgatc
     8461 ggcctctcgt ccgtccacgc gtagcttcgg agggcccagc cgatcgcctt cctcaggaag
     8521 aactccttcg acccgatcga cggctcgatg agcccgaaga ggagatcgag gtcggtgtcc
     8581 ttcttgaacg tgacctggca gaggatcgcg ctccgccgtt tccacatgtc cgggccgcgg
     8641 ctccatgcgc gcatcgcctt ccgcatcggc gcgggctcgt tccggaggat ctcgccgagg
     8701 cgcttcgtcg cgagggcgtc cacgaggtcc caccacgcgc ccgtcacgat catctcctcg
     8761 tacatcggga gggacgccat cgtctggaac gggcgggcgc ggcggtcgcc cgtcagctcg
     8821 atcgcggcgt atcgctcctc gcggaaccgc gcgccgcgcc agagcgcgag gacgtccttc
     8881 cgccacgact ccggatcctc gacgggatgc gcggcgaacg ccttccggca gatcgcgcgg
     8941 agcggcaccg cgccgactcc atggaacggc atcgcggact tcatgtacgc ccgcgcgccc
     9001 gcggcccgct cgggcgaccc ggcccgggcg agctcccggc ggaggacggt gaggaggcgc
     9061 gtggcgcagg ttcgcgtccc acgacgtccg gccggcgccg caaggcgagc gctcattttc
     9121 cgacggccga ccggaagtag aagtcgagca tggcgtccag cggcgctgca ttcccccgca
     9181 gcctgctgcg aagcgctgcg ccctcccagc agtcaacgag aacgctcgcc atgcgccgcg
     9241 tatccgagcc ggaccggatg tcgcccctcc ttcgggcctc ttccaggcaa accgcgaggc
     9301 gccgggcgat ctcggagaag cagccctcga tcttgcgccg gaaggtgtcg ttcacaccgg
     9361 acagttcctg acccagtccc cccaggaggc agcccatata gccctctttc cggtagtgct
     9421 cctgcgtcag ctcgaagaag cggcgaacgc ggcccagcgg cggacggccc tcgtcgccaa
     9481 ggcaggcgtc gagggccgcg tggacgttct gcatgtaccg gtcgatgacc tggagggcga
     9541 agtcctcctt gtcccggaag tgatggtaga aggagccctt tggagtgccc gtggcggcga
     9601 ggagcccctg gacacccaga tcgttgtagc catgctccag gagcatcttc aatccggcgt
     9661 cgagcaggcg ctgcttggtc ggatgagtca tcggcggcgg gcagcgggcg ttcttcccgg
     9721 aaagggaatc agcctttcac cttctcgatc atgtgggcga tcacgtggcc gtgttcacgc
     9781 tgcggactga acatgacgat ctcggcatcg gcgtcgaccc tgacgttgtg gccgggcggc
     9841 cagtagaaga gatcattcgt cttcacggtt tcctgcgcgc ctttcgcatc ggtcgtcgtg
     9901 agctggccgc tcaggacgaa tccccagtgc ggacactggc agaggttgcc ttccagcccc
     9961 tgaaagaggg gcgtggtatc gacacccgcg gcgagcgtga agcactcgcc gctgagcgag
    10021 ccgaatccgc tcgcgtcgcc gaagtccgtt cgctggcgga tcacggcacc cggaatctgc
    10081 atcctgacgt ccacgtcgtt cttcgcaact cgcatggcca ccttcccttt tcggctgaaa
    10141 tccagcgact agaccgatcg gtctatttct cgctagccta cacccttccc gggctctgtc
    10201 aacgggaacg cgtgaaggcc ggacaggccc tgccgggcct caagcccggc ggtagcgctc
    10261 gcggatcgtc ggaatgaggt actccgagaa cgccgactcg aggccgtact tcggcgagaa
    10321 gccccagtcc gcgcgcgcgg cgctgtcgtc cacgtccgcg ggccacgagt ccacgatgcc
    10381 ctgccgcttg tcgtccggct cgaacgtgac gcgcgcggcc gggaacgaac tcaggacgag
    10441 gtcgcggatc tcaccggccg acggcgcgaa cgcgcccacg ttgtagacct gccgcgtgag
    10501 cgtccccgcg ggcgcggagg cgagctggag gagtgcgtcg acgccgtccg gcatcgccat
    10561 gaacgggatg cgcgtgtcct cgcggacgaa gcaggcgtag ggcttcccct cggccgccgc
    10621 gtggatcatc tcgggcgcgt agtcggacgt gccgcccgac gggaccgtga cggccgagat
    10681 gaggccgggg aagcggaggc aacggaagtc cacgcgtccg ccctggttct cggacgagag
    10741 ctgcttgtag tgccgcgcgt agtagcgccc caggtgctcg gcgtagagct tgttgcagcc
    10801 gtacatcgtg gtcggcgtgt tgaactcgtc ctccttgacc ttcccggcca gcgccttggc
    10861 cgcgaggtcg ggcaggccgt acgccgcgat ggaggacggg tagaggaacg tgaccggccg
    10921 gccgtgcgac tggcgctcgc tctgggcgaa ctccagcatg ttgatcatcc cctcgacgtt
    10981 gacctggtgc gccgtcacgg gcgtgaactc gccgcgcgtc gagagaagcg ccgcgaggtg
    11041 gaagatcatg tcgatctcgt actcggagag gatccgctcg agaacgctcc ggtcgaggac
    11101 ggagcccgtg aactcgcgcg cgacgaggcg cccgagcgac gggtcgaggg ccttgaggtc
    11161 gagcgtgagg atggggcgcg tgccttcggc cgcgaggcgc gtgatgaggc cgtggcccat
    11221 ctcgccgctc gcgcccgtga tgaggacgac ggggcggcgc atcaggccac cgtgaccttc
    11281 gtctcgaggt agacgtcctg gatcgcgttg agcagggcga cgccctcctt catcggcttc
    11341 tggaacgcct ttcgcccgga gatgaggccc atgccgcccg cgcgcttgtt gatgacggcc
    11401 gtcgcgacgg cctggccgag gtcgtccttg ccgcccgccg cgccgccgga gttgatgagg
    11461 cccgcccggc ccatgtagca gttgacgacc tgccagcgcg tccactcgat cgggtgcgcc
    11521 gggacgagct tcgcgtaggc ctcggcgttc ggcttgttcc cgaacgggag cgccgggaag
    11581 ccgccgtcgt tctcgggctg tttctgcttg atgatgtcgg cctcgatcgt cacgccgagg
    11641 tggttcgcct gagaggaaag gtccgccgag acgtggaagt ccttctcctt cgtcttgaac
    11701 tgcgggttcc ggaggtagca ccacaggacc gtgaacatcc cgtagtcgtg cgcctggtgg
    11761 aaggcgttcg agatctccgt gatctgccgg cgcgagttgt ccgacccgaa gtagatcgtg
    11821 gccccgattc ccgcggcgcc catgtcgaag gcctgcttga cggtcgcgaa gatcgtctgg
    11881 tcgtaggacg tcgggagcgt caggagctcg ttgtggttga tcttcacgat gaacgggatc
    11941 cggtgcgccc acgtgcgcgc gaccgagccg agcacgccga gcgtcgaggc cacggcgttg
    12001 cagccgccct cgtaggccag cttcacgatg ttctcggggt cgaagtactc cggcgagggc
    12061 gcgaaggacg ccgcgcccga gtgctcgatg ccctggtcga cgggcaggat cgagaggtag
    12121 ccggtgccgg cgaggcggcc
//
LOCUS       DAOMFY010000103        12091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1636_length_12091_cov_8.475739, whole genome shotgun sequence.
ACCESSION   DAOMFY010000103 DAOMFY010000000
VERSION     DAOMFY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1636_length_12091_cov_8.475739"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLB01_10070"
     CDS             <1..860
                     /locus_tag="PLB01_10070"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQQ77689.1"
                     /translation="CLRCKPSAAEPPEAVRAVRRAVAFLEAHADESVPLATLARVAGL
                     SPFHFQRTFKRLVGVSPKRYADAQRADRLKALLRDGAPVASASFEAGYGSSSRAYASA
                     PRHLGTTPAAYARGGKGLHVRYATVQTPLGRLLVAATERGVCAVTLGDSERALEAGLR
                     REYPEALLEKAPGALAEWTQAIALSLAEAVDLSSIPLDLRATTFQRRVWEALRAIPRG
                     ETRSYSELAAAIGQPSAARAVASACAHNPAALVVPCHRIVRSDGALGGYRWGVARKRE
                     LLKKESGRP"
     gene            complement(892..1338)
                     /locus_tag="PLB01_10075"
     CDS             complement(892..1338)
                     /locus_tag="PLB01_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77690.1"
                     /translation="MNTKMRNLTLTAAALAALTVTAPKAAAEPWKNFPLPPSPHEVRE
                     MWRERHETPRQEYREHGRGGYGYGHRYGYGHGPVYVAPRPVVRYGYGYAAPYRTYAGF
                     RFYSACPGPGYVYVANYGWARPPFFGAVWVPGYTDIDGFWVEGYWR"
     gene            complement(1496..1774)
                     /locus_tag="PLB01_10080"
     CDS             complement(1496..1774)
                     /locus_tag="PLB01_10080"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HQQ77691.1"
                     /translation="MSRRNLLILGTAAIAAAVLFFFDPATTRFYPPCLFKTVLGTQCP
                     GCGSLRAAHQLLHGNLEEAWRLNKPLLIALPLAAAISLFTFLRNPKAP"
     gene            complement(1771..3819)
                     /locus_tag="PLB01_10085"
     CDS             complement(1771..3819)
                     /locus_tag="PLB01_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQQ77692.1"
                     /translation="MSDKEFKPFVPADSQMREFTGRALVLGLLMSVILGAANAYLGLK
                     AGMTIAATYPAAVIGMAVLRIFKGSLLEENFARTVGSIGESVAAGAIFTIPAFVILGM
                     WKFQGWNASEYLTATALMILGGFLGILFVTVLRRVMVEDKSLPFPESVAAAEIHKAGQ
                     RGAEAAQQLFKAMGVGALIKFFGDSGFFKASNDFHLSVGTYKESFVRLGLKGDAHKIE
                     AGGVTTMSAPAATPAYFGVGYIIGPELGALNFAGGLLAWGLFVPLLVFFLGPSHIGAY
                     TGADGTQNWAGLAGHLYRYIVRPIAVGGMLVGACFTLFRMRKNLIAGIKRGVQDVKKS
                     AAASAATSRIEKDLSLKVVGLGIAAVFVLMVGLYFYFTGVLGGAFLAALVMLVTGFFF
                     AAVSGNLVGMIGSSNNPISGLTLATTIVAALTMVIIGVKGAQGVAAVLGVAAVVCVSS
                     AVAGEMLQDLKVGHILGGTPWKMQLGDILGVLVAGLVMFFPLYLLHTSDLASNPGVGG
                     FGGKNLPAPQAGLMAALSQGIVGGEMAWPLVLVGIAMGLSLILIRVKSPMLFSVGMYL
                     PLETTFAIFIGGLIKGLVTKSADRRGYNEAQKARVENAGVLAASGLIAGEALVGLLVA
                     GVVAFRSDNRFPQIGGMFDAVGGWLAIPIMLVLAAYLVWVPLGKAGAADEPAPPTAVM
                     "
     gene            4030..4440
                     /locus_tag="PLB01_10090"
     CDS             4030..4440
                     /locus_tag="PLB01_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77693.1"
                     /translation="MNRTVLVLTDDLFWRTKIDHALKSSQTPGVFLDTPAEIEKKADP
                     TRVGLVLIDLALKSDPIAAITALKKAPRTKNIMVVGYCEHKQLELIERAKKSGCDQVL
                     MRSTFSQNLGDIILKYALPGSVRQESEEVELPEE"
     gene            complement(4450..4896)
                     /locus_tag="PLB01_10095"
     CDS             complement(4450..4896)
                     /locus_tag="PLB01_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HQQ77694.1"
                     /translation="MSRALGGFLPDTSCIVAALVDWHERHEAAAREISSRLDAGERFL
                     VAAPALVETYAVLTRLPAPHRLSPGDAVALLEANLFGHPLVALEAVSYRALIAEAPAR
                     SIAGGRTYDAVIARCAVRAGADAILTLNDRHFRGLAPGIEIVTPAS"
     gene            complement(4898..5155)
                     /locus_tag="PLB01_10100"
     CDS             complement(4898..5155)
                     /locus_tag="PLB01_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQQ77695.1"
                     /translation="MKTTIDSAGRLVIPREARREAGLVAGATLDVRVSEGQVILEPET
                     RPVRLVRKGRLTVARAADGGKGPRLTTEGVERTRSILRRRG"
     gene            5198..6307
                     /locus_tag="PLB01_10105"
     CDS             5198..6307
                     /locus_tag="PLB01_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HQQ77696.1"
                     /translation="MNSSRRRFLQAAAAAAAASALPLPSSPFEEKLQAQSPSTTGNAP
                     AADGYVLVTSGNGVEAAKAARQALIQGYDPLDAAIVGVNVNELDPNDMSVGYGGIPNE
                     EGVVQLDAAVMDGRTLRAGAVGALEGVKTPSKVAKLVMERTDRVFLVGAGARKFATAH
                     GFPVEDLLTEKTRKIWLYWRERLSDSDDWIEAGKEAEDPDIKEFIRKYGSDFFRPQGT
                     IHMSVRSAKGDLVGVTTTSGLFFKIPGRLGDSPVVGAGLYSENGVGSCGSTGRGEANL
                     LTCGSHTVVEFMRQGKSPEEACLLALKRIDEKTHRVPRHRDDKGRPKFNVSFYAVDAK
                     GRTAGAAIWSGSKYVVGDDAGVKTLESAYLYKRGS"
     gene            complement(6309..7025)
                     /locus_tag="PLB01_10110"
     CDS             complement(6309..7025)
                     /locus_tag="PLB01_10110"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQQ77697.1"
                     /translation="MPLAGHLVTIDLAKSGPLVIAVDRGEDGALRAATVRDLAAKSGL
                     ALAVNASFFEVPKGKYPVAGERADAVGRLVLDGKTTGAKGTIGGLLDGALCFDAHAAR
                     VEKGFACAGARWGLATGPVLLWKGTRTDTAFADAIFKAPRHPRTAAGVDARGTTLWLL
                     VVDGRQPGVSEGATLDELKDILADAGATDAINLDGGGSSTLVERQPDGSYKVLNVPTH
                     ERTPGRERPVVNAVGVRKGP"
     gene            complement(7171..7752)
                     /locus_tag="PLB01_10115"
     CDS             complement(7171..7752)
                     /locus_tag="PLB01_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HQQ77698.1"
                     /translation="MKGPLDLSRRRLCVSALAAAAASLLPKPLMASLEKLARESAGEK
                     DVRALAFHNTHTDERIEVVYGVAGRYRESGLTAIDWILRDHRTGDVARIDPRLLDLLH
                     LLKEQAAGPDAPAVFEIVSGYRSPRSNELLRNRSGAVARHSLHLKGMAADVRLTGVPL
                     ARLHAAALDLRRGGVGYYTDQFLHVDTGRVRSW"
     gene            7836..8975
                     /locus_tag="PLB01_10120"
     CDS             7836..8975
                     /locus_tag="PLB01_10120"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQQ77699.1"
                     /translation="MLSICALPARAQAPVPQRVAQYDWLAAHLPRYRELAAKPWPGPL
                     PAVKKLSPGDPYAGVTALAAILRDLGDLPASSAVPADGTYGGGLVDAVKAFQGRHGLR
                     PDGVLGAGTFVALNHPYTKRLQEIEESLKWLETIPVPEAHALVVVNIPSFQLYAWTAA
                     DRGKKASLTMPVVVGKAAKSATPILGGEIAHLVFRPYWYVPRSVVVKEMLPAYAKNPA
                     YFEKHELELTATNDDTKPGVATTPENVELLRAGKLGVRQKPGPRNSLGKVKFIFPNPE
                     SVYLHDTPAKSLFERDRRDFSHGCVRVSDPSALAEFLLRGAPGWDAARIAAAMEAERP
                     QVVLLKPRVPVWLIYTTAIASADGTLAFFEDVYRKGAPAGGPGAR"
     gene            complement(8976..9680)
                     /locus_tag="PLB01_10125"
     CDS             complement(8976..9680)
                     /locus_tag="PLB01_10125"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQQ77700.1"
                     /translation="MANERLLPSVLTAAGIVFAGFLVGDAIRYTKKFESAVEVRGLDE
                     RLVRSDRASWQLNLAVTAATLPAANDALVESGKALREFLASQGFDERELRAGPTYVSD
                     AWANGMPNARPESRYTVRGGVVLETAKVDAVEKVSQSTGDLLKRGIRVDNSNVRYYFT
                     DLNKVKTEMLAAATANARQAAEAFAKDSKTRLGGIKSASQGLFTIGAPLAEYEGETSV
                     MKKVRVVTRVQYFLEK"
     gene            complement(9683..11689)
                     /locus_tag="PLB01_10130"
     CDS             complement(9683..11689)
                     /locus_tag="PLB01_10130"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77701.1"
                     /translation="MDPLALRDMGERLDTFISAQGVGDRSDAFVRLVRSARVIAAEPA
                     AAIPLVDALNADVAWKSRFQDALAALLAETDATALFGETGIPAERGFFAELSDRVSSR
                     LLPEPRDDRDLSRVVRRLFRSASDVDSLKERDPRVFHGLVELFAGAGAKAWAPMRQGF
                     ADGFRLLAARVLAHGLSRDLRARGTGKRVADSPFYRLKGSADALLDAWSAGGDVPAAS
                     AAFRKESGACRKEARAVQQGLEDAGVSVQSVFDLEVVDRCLTRMALMADVIDAAPGIE
                     TSRAAHRLLVRLATLAQQDRSVRHLVKWNLHLLDRKIVERSGETGEHYVAADKKDYRH
                     IWVAAAGGGALTVLTAAIKMAVHELHLAPFPESFLYGLNYAVSFLGLQAFGLILATKQ
                     PAMTAATLAKIIQSSEGEDRLERVATFFSRLTSSQFAAAAANVLVVGAGAAVFDGLWR
                     LAAGSPFLDAEVAAHTFETLSPLDSGTVFFAALTGVLLWMGSVAGGWFENWVTVHRLP
                     EALSEKKPGGVLSRERLQRAGAALSRNAAGWGTNVALGFLLGFAPAVGRFFGIPFDVR
                     HVTLSTGTLALAAAGLGERWFLGGWFLRALGGIAVMFVLNLTVAFALSLVNAARAFEM
                     TNEEILGVLRHVFRHFRKNPFSFVLPPKAAPAFADASVPDGPKE"
     gene            complement(11694..>12091)
                     /locus_tag="PLB01_10135"
     CDS             complement(11694..>12091)
                     /locus_tag="PLB01_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HQQ77702.1"
                     /translation="LGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFVADSQE
                     ARMDANIESLQNLDKNLTEHGFDLQVIPYVLQLNKRDLPTAMPAEELYRQLNFKGEPT
                     FEATAPTGQGVFDTLKAVAKQVLMELRKK"
BASE COUNT         1887 a         4264 c         4248 g         1692 t
ORIGIN      
        1 cgtgcctgcg ctgcaagccg agcgcggccg agccgcccga ggccgttcgc gccgtccggc
       61 gggccgtcgc gttcctcgag gcccacgcgg acgagagcgt cccgctcgcg acgctcgcgc
      121 gcgtggcggg cctgtctccg ttccacttcc agaggacgtt caagaggctc gtcggcgtct
      181 cgccgaagcg gtacgccgac gcgcagcgcg ccgaccgtct caaggcgctc ctcagggacg
      241 gcgctcccgt cgcctccgcg agcttcgagg ccggctacgg ctcgtccagc cgcgcgtatg
      301 cgagcgcgcc gcgccacctc gggacgacgc ctgcggcgta cgcgcgcggc ggcaaggggc
      361 tgcacgtgcg ctacgcgacg gtccagacgc ccctcgggcg gctcctcgtc gccgcaacgg
      421 agcgcggcgt ctgcgccgtg acgctcggcg actccgagcg cgcgctcgag gcgggcctca
      481 gacgcgagta ccccgaagcg ctcctcgaga aggcgcccgg ggcgctcgcg gagtggacgc
      541 aggcgatcgc gctctcgctc gccgaagccg tcgacctctc gtcgatcccc ctcgacctgc
      601 gcgccaccac cttccagcgc cgggtgtggg aggcgctgcg cgcgatcccg cgcggcgaga
      661 cacgctcgta ctccgagctc gccgccgcga tcggccagcc ctcggccgca cgcgccgtcg
      721 cctcggcctg cgcgcacaac cccgcggccc tcgtcgtccc gtgccaccgc atcgtgcgga
      781 gcgacggcgc cctcggcggc tatcgctggg gcgtcgcgcg gaagcgagag ctcctgaaaa
      841 aggaaagcgg gcggccctga ggccgcccgc cgtctccggg aaccgggccg ttcagcgcca
      901 gtacccttcg acccagaacc cgtcgatgtc ggtgtagccg gggacccaga ccgcgccgaa
      961 gaagggcggc cgggcccagc cgtagttcgc gacgtagacg tagccggggc cggggcacgc
     1021 ggagtagaag cggaagcccg cgtacgtccg gtacggtgcg gcgtagccgt agccgtagcg
     1081 gaccaccggc cgcggggcca cgtagacggg cccgtggccg tagccgtacc ggtggccgta
     1141 gccgtagccg ccgcgcccgt gctcgcggta ctcctgcctc ggcgtctcgt gccgctcgcg
     1201 ccacatctcc ctcacttcgt gcggcgacgg aggcagcgga aagttcttcc agggctccgc
     1261 ggcggctttc ggagcggtca ccgtcagggc cgcgagggcc gcggcggtga gcgtcaggtt
     1321 gcgcatcttc gtgttcatga ccggcctctc tttctgcccc gggcggcgtc gccccggacg
     1381 attcgataag agcaaggccg tgccaacccc cgcaccgtga ttcccgtcac gcgccgcacg
     1441 ctttggtgca gcgaagaggg gacagtgcac ctttctgtga cgcctcttca ggtcgtcagg
     1501 gcgctttcgg atttctcaga aaggtgaaga gggaaatcgc agctgccagc ggcagcgcga
     1561 tcagcagagg cttgttgagc ctccaggctt cttcgagatt cccgtgcagg agctggtgcg
     1621 cggcgcgcag gcttccgcat cccgggcact gcgttccgag cacggtcttg aacaggcacg
     1681 gcgggtagaa gcgcgtcgtg gccgggtcga aaaagaagag gaccgccgcg gcgatcgcgg
     1741 cggtccccag gatcagcagg ttgcggcggc tcacatcacc gcggtgggag gagccggctc
     1801 gtcggccgcg ccggccttcc cgagcggcac ccacacgagg taggccgcga ggacgagcat
     1861 gatcgggatc gcgagccagc cgcccaccgc gtcgaacatc cccccgatct gcgggaacct
     1921 gttgtccgac cggaaggcga cgacgcccgc gacgaggagg ccgacgagcg cctcgcccgc
     1981 gatgaggccc gacgccgcga ggacgcccgc gttctcgacg cgcgccttct gcgcctcgtt
     2041 gtagccgcgc ctgtcggccg acttcgtcac gaggcccttg atcaggccgc cgatgaagat
     2101 cgcgaacgtc gtctcgagcg gcaggtacat gccgaccgag aagagcatcg ggctcttgac
     2161 gcggatgagg atgagcgaga ggcccatcgc gatcccgacg aggacgagcg gccacgccat
     2221 ctcgccgccc acgatgccct gcgagagcgc ggccatgagg ccggcctgcg gggccgggag
     2281 gttcttgccg ccgaagccgc cgacgccggg gttcgaggcg aggtcggacg tgtggaggag
     2341 gtagagcggg aagaacatga cgaggcccgc gacgaggacg ccgaggatgt cgccgagctg
     2401 catcttccac ggcgtgccgc cgaggatgtg cccgaccttg aggtcctgca gcatctcgcc
     2461 cgcgaccgcc gacgatacgc aaacgaccgc ggccacgccg aggacggccg cgacgccctg
     2521 cgcgcccttg acgccgatga tgaccatcgt gagcgccgcg acgatcgtcg tcgcgagcgt
     2581 gaggcccgag atcgggttgt tcgacgagcc gatcatcccg acgaggttcc ccgagacggc
     2641 cgcgaagaag aagcccgtca cgagcatgac gagcgccgcg aggaacgcgc cgccgaggac
     2701 gcccgtgaag tagaagtaga ggccgaccat gaggacgaag acggccgcga ttccgaggcc
     2761 gacgaccttc aggctgaggt ccttctcgat gcgcgacgtg gccgcggaag ccgccgccga
     2821 cttcttgacg tcctggacgc cgcgtttgat gccggcgatc aggttcttcc gcatccggaa
     2881 gagcgtgaag cacgcgccga cgagcatgcc gccgaccgcg atgggccgga cgatgtagcg
     2941 gtagaggtgg cccgcgagac ccgcccagtt ctgcgtgccg tcggcgcccg tgtaggcgcc
     3001 gatgtggctc gggccgagga agaagacgag gagcggcacg aagaggcccc acgcgaggag
     3061 gccgcccgcg aagttcaggg cgcccagctc gggcccgatg atgtagccga cgccgaagta
     3121 cgcgggcgtc gccgcagggg ccgacatcgt cgtcacgccg ccggcctcga tcttgtgcgc
     3181 gtcgcccttg aggccgaggc gcacgaagct ctccttgtac gtcccgaccg agaggtggaa
     3241 gtcgttcgag gccttgaaga accccgagtc gccgaagaac ttgatgagtg ctccgacgcc
     3301 catcgccttg aagagctgct gcgccgcctc ggcgccgcgc tggccggcct tgtggatctc
     3361 ggccgccgcg acggactcgg ggaacggcag cgacttgtcc tcgaccatga cgcgccggag
     3421 caccgtcacg aagaggatgc ccaggaaacc gcccaggatc atgagggccg tcgcggtcag
     3481 gtactcgctc gcgttccatc cctggaactt ccacatcccg aggatgacga acgccgggat
     3541 cgtgaagatc gcgccggcgg caaccgactc gccgatcgac ccgacggtac gggcgaagtt
     3601 ctcctcgagg agggagccct tgaagatccg gaggaccgcc atcccgatga cggccgccgg
     3661 gtacgtcgcg gcgatcgtca tgccggcctt caggccgagg tacgcgttcg cggcgccgag
     3721 gatgacgctc atgaggaggc ccaggacgag ggcccgcccc gtgaactccc gcatctggga
     3781 gtcggcgggg acgaacggct tgaattcctt gtcgctcatg cggaaaaaac cctccttgca
     3841 ccgcgggtcg cgggtccggc cggccggtgc attcggcagt attcggtcat ggaaacgccc
     3901 ggagtctgac actcccgcgc ccggattgtc gaacgggaaa cgcctcacgc ggctatactt
     3961 cagggtgact cagtcccatc agacggtccg gattgccggg tccggggtct gtttctccga
     4021 ggagggacca tgaaccgcac cgtgctcgtc ctgacagacg acctcttctg gagaacgaaa
     4081 atcgatcacg cgttgaagtc cagccagacg ccgggcgtct tcctcgacac gcccgccgag
     4141 atcgagaaga aggccgaccc gacgcgcgtc ggcctcgtgc tgatcgacct cgccctcaag
     4201 agcgacccga tcgccgcgat cacggcgctc aagaaggccc cccggacgaa gaacatcatg
     4261 gtcgtcggct actgcgagca caaacagctc gagctgatcg agcgcgccaa gaagtccggc
     4321 tgcgaccagg tgctcatgcg ctcgacgttc tcccagaacc tcggcgacat catcctgaag
     4381 tacgcgctgc ccggcagcgt ccgccaggag tccgaggaag tcgagctgcc cgaggagtag
     4441 ggccggcggt caggacgccg gcgtcacgat ctcgatgccc ggagccaggc cgcggaagtg
     4501 ccggtcgttg agcgtcagga tcgcatcggc gccggcacga accgcgcacc gagcgatcac
     4561 ggcgtcgtag gtgcggcccc cggcaatgga acgggccggc gcttccgcga tgagcgcccg
     4621 atacgacacc gcctcgagag ccacgagcgg gtgcccgaag agattcgcct caagaagagc
     4681 gaccgcgtcg ccgggtgaga gacggtgcgg cgcggggagc cgtgtgagaa ctgcgtaagt
     4741 ctccacgagg gctggcgccg cgacgaggaa ccgttcaccc gcgtcgaggc gcgaagagat
     4801 ttcgcgagcc gctgcctcgt gccgttcatg ccaatcgacc agcgccgcga cgatgcaact
     4861 cgtatcgggg agaaagcccc cgagagctcg ggacacgtca gccccggcgc cgcaggatcg
     4921 atctcgtccg ctcgacgccc tcggtcgtca gtcgcgggcc cttcccgccg tcagccgcgc
     4981 gcgcgaccgt gagacggccc tttcgcacga ggcggacggg acgtgtctcc ggctcgagaa
     5041 tcacctggcc ctccgagacg cgtacgtcga gtgtcgcgcc cgcgaccagg cccgcctcgc
     5101 ggcgagcctc tcgcgggatg acgaggcgcc cggcggaatc aatggtggtt ttcatggtaa
     5161 ggattctacc acttcgatct cggctatcgt ctcccgcatg aattccagcc gccgccgctt
     5221 cctccaggcc gctgcagcgg ccgcagccgc ctcggccctt cccctcccct cttccccctt
     5281 cgaggaaaaa ctacaggcgc aaagccccag cacgaccggc aacgcgccgg ccgccgacgg
     5341 ctacgtcctc gtgacgtccg gcaacggcgt cgaggccgcg aaggccgcgc gccaggcgct
     5401 catccagggc tacgacccgc tcgacgccgc gatcgtgggc gtgaacgtga acgagctcga
     5461 cccgaacgac atgagcgtcg ggtacggcgg gatcccgaac gaggagggcg tcgtccagct
     5521 ggacgcggcc gtcatggacg ggcgcacgct gcgcgcgggc gccgtcggcg cgctcgaggg
     5581 cgtcaagacg ccgtcgaagg tcgcgaagct cgtcatggaa cgcacggacc gcgtgttcct
     5641 cgtgggcgcg ggcgcgcgca agttcgcgac ggcgcacggc ttccccgtcg aggacctcct
     5701 gacggagaag acgcggaaga tctggctcta ctggagagag cgcctgtcgg acagcgacga
     5761 ctggatcgag gccggcaagg aagccgaaga tccggacatc aaggagttca tccgcaagta
     5821 cggcagcgac ttcttccggc cgcagggcac gatccacatg tcggtccgca gcgccaaggg
     5881 cgacctcgtc ggcgtcacga cgacgtccgg gctcttcttc aagatccccg gccggctcgg
     5941 cgactcgccg gtggtcggcg cgggcctcta ctccgagaac ggcgtcggga gctgcggctc
     6001 cacggggcgc ggcgaggcga acctcctgac gtgcggctcg cacacggtcg tcgagttcat
     6061 gcggcagggc aagagccccg aggaagcctg cctcctcgcg ctcaagcgca tcgacgagaa
     6121 gacccaccgc gtcccccgcc accgcgacga caagggccgc ccgaagttca acgtcagctt
     6181 ctacgcggtc gacgcgaagg gccggaccgc cggcgccgcc atctggagcg gcagcaagta
     6241 cgtcgtcggc gacgacgccg gcgtgaagac gctggagtcg gcgtacctgt acaagcgggg
     6301 aagctgaact agggcccttt cctcaccccc accgcattca ccaccggtct ttcgcggcca
     6361 ggtgttcgtt cgtgggtggg gacgttgagc accttgtagc tgccgtcggg ttggcgctcg
     6421 accagcgtcg agctgccgcc gccgtcgagg ttgatggcgt ccgtcgcgcc cgcgtccgcg
     6481 aggatgtcct tcagctcgtc cagcgtcgcg ccttcggaga cgcccggctg cctgccgtcg
     6541 acgacgagga gccagagcgt cgtgcccctt gcgtcgacgc ccgccgccgt gcgcggatgg
     6601 cggggagcct tgaagatcgc gtccgcgaac gcggtgtccg tgcgcgtgcc cttccagagc
     6661 agcaccgggc ccgtcgcgag gccccagcgc gctcccgcgc atgcgaaccc cttctcgacg
     6721 cgcgccgcgt gcgcgtcgaa gcacagcgct ccgtcgagca ggccgccgat cgtgcccttt
     6781 gcgcccgtgg tcttcccgtc gaggacgagc ctgcccacgg cgtcggcccg ctcgcccgcc
     6841 acgggatact tccccttcgg cacctcgaag aagctcgcgt tcaccgcgag cgcaaggccg
     6901 ctcttcgccg cgaggtcgcg cacggtggcg gcgcgcagcg cgccgtcctc gccgcggtcc
     6961 acggcgatta ccagcggccc ggacttcgcg aggtcgatcg tgacgagatg gcccgcgagc
     7021 ggcacgggtt ccttccgctc gaccttccgg atcgcgacgc cggagaggcc ggccgcgggt
     7081 gaggccgcgc gtggcggcgc gagcgtcgtc gtgcacgcga cgagaagcgc cagcgagaag
     7141 gcgggagcga ggcggcggag cttcagagcc ctaccagctg cggacgcggc cggtgtcgac
     7201 gtgcaggaac tggtccgtgt agtagccgac gccgccgcgc ctgaggtcga gcgcggccgc
     7261 gtggaggcgc gcgagcggaa cgcccgtgag ccgcacgtcg gccgccatgc ccttgaggtg
     7321 caggctgtgc cgcgcgacgg cgccggagcg gttgcggagc aactcgttcg agcgcggcga
     7381 gcggtagccc gagacgatct cgaagacggc cggcgcgtcc gggcccgccg cctgctcctt
     7441 caggaggtgg agcaggtcga ggagcctcgg gtcgatgcgc gccacgtcgc cggtgcggtg
     7501 gtcgcgcagg atccagtcga tcgccgtgag gcccgactcc cggtagcggc ccgcgacgcc
     7561 gtacacgacc tcgatgcgct cgtcggtgtg cgtgttgtgg aacgcgagcg cgcgcacgtc
     7621 cttctccccc gcggactcgc gggcgagctt ctccagcgag gccatgagcg gcttcgggag
     7681 gagggaggcg gccgcggcgg cgagggccga gacgcagagg cggcgccgcg aaaggtcgag
     7741 agggcccttc aagaccggcg attttgacag cggggagcgc atgggacaac ctgcggcccg
     7801 caatgcgaag actcaccttt gttttcagtg ctttggtgct ctcgatctgc gcgcttccgg
     7861 cgcgggcgca ggctcccgtc ccccagcgcg tcgcgcagta cgactggctc gccgcgcacc
     7921 tcccgcgcta ccgcgagctc gcggcgaagc cctggcccgg gccgcttccg gccgtgaaga
     7981 agctctctcc cggcgatccg tacgcgggcg tgaccgcgct cgccgccatc ctgcgcgacc
     8041 tcggggacct gcccgcctcc tcagccgtcc ccgcagacgg cacgtacggc ggtgggctcg
     8101 tcgacgccgt gaaggcgttc cagggccgcc acggcctcag gcccgacggc gtcctcggcg
     8161 ccgggacgtt cgttgcgctc aaccacccgt acacgaagcg gctccaggag atcgaggagt
     8221 ccctgaagtg gctcgagacg atccccgtgc cggaggccca cgccctcgtc gtcgtgaaca
     8281 tcccgtcctt ccagctctac gcgtggacgg ccgccgaccg cgggaagaag gcgtcgctca
     8341 cgatgcccgt cgtcgtcggg aaggccgcga agtcggcgac gccgatcctc ggcggcgaga
     8401 tcgcgcacct cgtcttccgg ccgtactggt acgtgccgcg cagcgtcgtc gtgaaggaga
     8461 tgctcccggc gtacgcaaag aacccggcgt acttcgagaa gcacgagctc gaactcacgg
     8521 cgacgaacga cgacacgaag ccgggcgtcg cgacgacgcc ggagaacgtc gagctgctgc
     8581 gcgccggaaa gctcggcgtc cgccagaagc cgggcccgcg caactcgctc ggcaaggtga
     8641 agttcatctt cccgaacccc gaatcggtct atctccacga cacccccgca aaatcgctct
     8701 tcgagcgcga ccggcgcgac ttctcgcacg gctgcgtgcg cgtctccgac ccgtccgccc
     8761 tcgcggagtt cctcctgagg ggcgcccccg gctgggacgc cgcgaggatc gccgcggcca
     8821 tggaggccga gcgcccgcag gtcgtcctcc tcaagccgcg tgtgcccgtc tggctcatct
     8881 acacgacggc gatcgcgagc gccgacggga cgctcgcctt cttcgaggac gtctacagga
     8941 agggcgcgcc cgcgggcggg ccgggcgcgc gctgactatt tctcgaggaa gtactgcacc
     9001 cgcgtgacga cgcggacctt cttcatgacg gacgtctcgc cctcgtactc ggccagcggc
     9061 gctccgatcg tgaagagtcc ctgcgacgcg gacttgatgc cgccgagcct cgtcttcgag
     9121 tcctttgcga acgcttcggc cgcctgacgc gcattcgcgg tcgcggccgc gagcatctcc
     9181 gtcttcacct tgttgaggtc cgtgaagtag tagcggacgt tgctgttgtc gacgcggatc
     9241 ccgcgcttca gcaggtcgcc ggtcgactgc gagaccttct cgacggcgtc caccttcgcg
     9301 gtctcgagga cgacgcctcc gcgcaccgtg tagcggctct ccggccgggc gttcggcatc
     9361 ccgttcgccc acgcgtccga cacgtacgtc ggtcccgctc ggagctccct ctcgtcgaag
     9421 ccctgcgacg cgaggaactc ccgcagagcc ttgccgctct cgacgagcgc gtcgttcgcg
     9481 gccggaagcg tcgccgccgt gacggcgagg ttgagctgcc agctcgcgcg gtccgagcgc
     9541 acgagccgct cgtcgaggcc ccgcacctcg acggcgctct cgaacttctt cgtgtaccgg
     9601 atcgcgtcgc cgacgagaaa gcccgcgaag acgatcccgg cggccgtgag gacggagggc
     9661 agcaggcgtt cgttggccat ggtcactcct tggggccgtc cgggacggac gcgtcggcga
     9721 acgccggcgc ggccttcggc ggcaggacga acgagaacgg gttcttccgg aagtggcgga
     9781 acacgtggcg gaggacgccg aggatctcct cgttcgtcat ctcgaacgcg cgcgccgcgt
     9841 tgacgaggga cagcgcgaac gcgacggtca ggttcaggac gaacatcacg gcgatgccgc
     9901 cgagcgcgcg caggaaccag cccccgagaa accacctctc cccgagcccc gcggccgcga
     9961 gcgcgagcgt gcccgtcgag agcgtgacgt gccggacgtc gaacgggatc ccgaagaagc
    10021 ggcccacggc cggcgcgaac ccgagcagga atccgagcgc gacgttcgtc ccccatccgg
    10081 ccgcattccg gctcagcgcc gcgccggcgc gctggagacg ctcccgggag aggacgccgc
    10141 ccggcttctt ctccgagagc gcctcgggca gccgatgaac ggtgacccag ttctcgaacc
    10201 agccgcccgc gacgctgccc atccagagga ggacgcccgt cagcgcggcg aagaagaccg
    10261 tcccgctgtc gagcgggctg agcgtctcga aggtgtgggc ggcgacctcc gcgtcgagga
    10321 acgggctgcc cgccgcgagg cgccagagcc cgtcgaagac ggctgcgccc gcccccacga
    10381 cgaggacgtt cgcggccgcg gccgcgaact gcgagctcgt gagacgggag aagaacgtcg
    10441 cgacccgctc gagccggtcc tcgccctcgc tcgactggat gatcttcgcg agcgtcgcgg
    10501 ccgtcatggc gggctgcttc gtcgcgagga tcaggccgaa cgcctggaga ccgaggaagc
    10561 tcacggcgta gttcaggccg tagaggaagc tctcgggaaa cggcgcgagg tggagctcgt
    10621 ggacggccat cttgatcgcg gcggtgagga ccgtgagcgc accgccgccc gcggccgcga
    10681 cccagatgtg ccggtagtcc ttcttgtccg ccgcgacgta gtgctcaccc gtctcgcccg
    10741 agcgctcgac gatcttgcgg tcgagaaggt ggaggttcca cttcacgagg tggcggacgg
    10801 agcggtcctg ctgcgcgagc gtcgcgaggc gcacgagcag gcggtgcgcc gcgcgcgacg
    10861 tctcgatccc gggcgccgcg tcgatcacgt cggccatgag cgccatccgc gtgagacagc
    10921 ggtccacgac ctcgaggtcg aagacgctct ggacgctgac gcccgcgtcc tcgaggccct
    10981 gctggacggc ccgggcttcc ttgcggcacg cgcccgactc cttgcgaaag gccgccgagg
    11041 ccgcgggcac gtcgccgccc gcgctccacg cgtcgaggag cgcgtcggcg gagcccttca
    11101 ggcggtagaa cggcgagtcg gcgacccgct ttcccgtgcc gcgcgcgcgg aggtcgcgcg
    11161 agagcccgtg cgcgaggacg cgcgccgcga ggagccggaa gccgtcggcg aagccctgcc
    11221 gcatgggcgc ccaggccttc gctcccgcgc ccgcgaacag ctcgacgagg ccgtgaaaca
    11281 cgcgcgggtc gcgttccttc agggaatcga cgtcggaggc gctcctgaac aggcgccgca
    11341 cgacgcgcga gaggtcccgg tcgtcgcgcg gctcgggcag gaggcggctc gagacgcggt
    11401 cggacagctc ggcgaagaat ccacgctcgg ccggaatgcc cgtctcgccg aagagtgccg
    11461 ttgcgtcggt ttccgcgagg agcgccgcga gggcatcctg gaaacgcgac ttccacgcga
    11521 cgtccgcgtt cagggcgtcg acgagcggga tggccgcggc cggctcggcg gcgatgacgc
    11581 gcgcggacct cacgagcctc acgaacgcgt cggagcggtc gcccacgccc tgggcggaga
    11641 tgaacgtgtc gagacgttcg cccatgtccc ggagcgcgag cgggtccacg gggctacttc
    11701 ttccgcagct ccatgaggac ctgcttcgcc acggccttga gcgtgtcgaa gacgccctgt
    11761 ccggtcggcg ccgtcgcctc gaacgtcggc tcgcccttga agttcagctg gcgatacagc
    11821 tcctcggcgg gcatcgccgt cgggaggtcg cgcttgttga gctggaggac gtacgggatg
    11881 acctggaggt cgaacccgtg ctccgtgagg ttcttgtcga ggttctggag cgactcgatg
    11941 ttcgcgtcca tgcgcgcttc ctgcgagtcg gccacgaaga cgacgccgtc gacgcccttg
    12001 aggatgagct tgcggctcgc gtcgtagaag acctgtccgg ggaccgtgta gaggtggaac
    12061 cgcgtcttga agccccgcac cgtcccgagg t
//
LOCUS       DAOMFY010000104        11992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1648_length_11992_cov_6.966910, whole genome shotgun sequence.
ACCESSION   DAOMFY010000104 DAOMFY010000000
VERSION     DAOMFY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11992
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_11992_cov_6.966910"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="PLB01_10140"
     CDS             <1..1549
                     /locus_tag="PLB01_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77703.1"
                     /translation="RIAKARFAPSGQDVIYDASWQGEPWQLFATPIVDPKERSLGQPD
                     VQLLDLSRAGELVLRLKSKVADTWQFEGTLARSALGGDLAPKAILGGVLQAAWAPKSE
                     FAVVRATGGRSLLDYPPGKVRVESPGWIGDLRFSPDGSMLAYVDHANAGDDAGRVAVL
                     DVAAGKTRFLSKEWGTLRGLAWKGKEIWFTAGTHILNRALYAVDLSGKERGIFTQAGG
                     LHLHDVASDGRVLMSREEMRGGLLSQTEGVLQPLDLSWRTWSYLLDTSADGKTLLFEE
                     EEGDPAGYDLFLRPTSGGHATPLGKSAGYGALSPDGTLAVAWLAKPAPRWVLYPTGTG
                     APREIPAHGIRSIKSVSFSKDGRSLIFIGPEGEKEDRVWSLALDGKEPKPLTLEGVTG
                     LETWPLLSPDGKSFLAREKGGWSIFDLAKPDAPARRVVGLEDGEVLTQWRADGRFFVT
                     RPGAFPVDVWMLDPATGKRQPWKTVSPAGYVGADCSTIRFSEDGKRMAARYILQRSTL
                     YVVEGLR"
     gene            complement(1553..2686)
                     /gene="hisC"
                     /locus_tag="PLB01_10145"
     CDS             complement(1553..2686)
                     /gene="hisC"
                     /locus_tag="PLB01_10145"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQQ77704.1"
                     /translation="MTVPMSRRAFAGSLGAAAGVAFLDTPLVGRVAAAATKRPRPADA
                     VVLSSNENPYGPSPKALEAAAKAATNRYPDALENAAVEAIAKHHGVAPEQVLLGCGSS
                     EILQMADEAFSGPGKKVVAAEPTFEAVLAYAKVVKADGIKVALTPDFRHDLAKMAAAC
                     DASTGLVYVCNPNNPTATIVTGDEMAAFASAVPASAAILVDEAYHHFVEDPRYRSSLE
                     LIAKRPNVVVARTFSKIYGMAGMRLGYAVSSKETIAAMAPYASWSNANAGVLAAAAAS
                     LADPDLVPRQRKLLNDTRKWLVSELTKLGYRTMPSEANFVMIDVGGDVAPFIQAFRAR
                     NLLVGRKFPSLPNWLRVTIGKREEVEAFLAALPEVVPAPVRAA"
     gene            2783..5533
                     /locus_tag="PLB01_10150"
     CDS             2783..5533
                     /locus_tag="PLB01_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77705.1"
                     /translation="MTIASGTRLGPYEVLSLLGAGGMGEVYRAKDPRLNRDVAVKVLP
                     EDLFEGEERRQRFEREAKLLATLNHPNIAAIYSFEEISGRHLLTMELAAGEALDKKIS
                     SGPLPLEESLSLARQIAEALEAAHERGIVHRDLKPANVVVSSEGKVKLLDFGLAKAFE
                     NENGSSPEISLSPTLTARATAAGVILGTAAYMSPEQARGKPVDKRADVWAFGVVLFEM
                     LTGKRLFQGETVSDTLAAVLRDPVDFGKLPPSTPPSVRVLLERCLERDPKQRLQAIGE
                     SRIALERTIAGSSGAMKAATDSGSAVAVAAPSPERRAFPWAIGAAVGALAVFALWAPW
                     KKAPRPAPVRLSSELGADASLVTDLGPAAVLSPDGTLLAFVAQKTGGGSAQIWVRRLD
                     QLPAAPLAGTEGAREPFFSPDGQWIGFLADGKLKKVSVTGGAAVVLCDAPNLRGAFWT
                     EDETIVFLPNSGPSVALVKVPAAGGKPEPFTKLEEGEVTQRWPQILPGGKAILYTGHA
                     TTSSGFEDANIVVQPLPKGPRKILQRGGYHGRYLPSGHVVYVHESTLFAAPFDLGRLE
                     LTGPPVPVVEGIASTPNSAGSNFAFSNGGTLVYLPGKSVGAAAPIHWMDREGKTSPLR
                     STASAWNDIRFSPDGQKVAMDVTDGGQTDVWVYEWARDTMSRFTFDLAEDSHAAWSPD
                     GKRIAFASARADKAVTNLYWQRSDGTGDVQRLTESKVRQISTSFHPGGRFLAFMEQDP
                     KTNWDIVILPIEGDEASGWRPGKPTVFLNSPFVEVWPEFSPDGRWLAYMSNESGRFEV
                     YVRPFPGPGGKWQISTEGGGFPTWSRASRELFFGQDGKIMAVPYSADAASFRAEKPQP
                     LSGGRYTTTGPFRAFDLHPDGKRFAILKPPDAQSDVRHDKVVFVFNFFDELKRVAGTG
                     KK"
     gene            5530..8286
                     /locus_tag="PLB01_10155"
     CDS             5530..8286
                     /locus_tag="PLB01_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77706.1"
                     /translation="MTIAAGTKLGPYEIVSPLGAGGMGEVYRAKDTRVDRVVALKVLP
                     EEFFEDEERRARFGREAKLLASLSNPGIAVLYSFEEIPGSSSPSSSRHLLAMELVEGE
                     DLAARIASGSLSLEESLSLARQIAEALEAAHEKGIVHRDLKPANVKITPEGRVKLLDF
                     GLAKIFESEATLSNPSISYSPTLTARGTAAGVILGTAAYMSPEQARGKPVDKRTDVWA
                     FGCVLYEMLAGKKAFEGETVSDTLAAILRGEPDWSALRAPVSSKVNELLRRCLQRDPK
                     QRLRDIGDARIALEEETAVSASRAGQLPFEENPALPSPAVASVERAGRERGSKKNLSV
                     FLPWVLAAAFAAAAGALALRARAPEARVYRSVLPPPEGTNFWLEANGPGPVVVSPDGR
                     QVAFTAADAAGKVNLYVRALDSREARMLSGTEAAMYPFWSPDGGSLGFFTTGKLKTIE
                     ATGGSPQTICGAPEGKGGTWSPAGVIVFTPGPTSPLFRVSEKGGETTAITKIDASRGD
                     DSHRHPRFLPDGKHFLYVARSTKTGSEGHYVVAASLDGGAEKVLLRSPAAAVYASGHL
                     LYLRETTLMSRPFDAARLAFTGEASPVAEKILMPAVGTALGVFSASQNGVLAYQTARG
                     ETTSRLQWFTRDGKPDGTLGEPADFGQALLSPDGKLAAATIRDQATGTYDIWIFDVAR
                     GVRTRFTFDPAEDVGPLWSPDGSALIFASNRKGHYDLYRKALDGSSEEEALFTSDADK
                     GPVSWAPGGRTLVFDEAGKDAGVEVKTLALDGARKPEPLSKTKFSEVNSPLSPDGRWL
                     PYSTDESGRWEVYVTSFPRAGRKWQVSTDGGAYAFWSADGKEILYHDLSGMIWAVPVS
                     ARGETFEIGQGRRLFRAPGPNPTAPSFSATSDHQRFLVVGEGQKPNALVDLVVNWTIA
                     KGSR"
     gene            8283..10985
                     /locus_tag="PLB01_10160"
     CDS             8283..10985
                     /locus_tag="PLB01_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77707.1"
                     /translation="MTIAAGTRLGAYEVLGLLGAGGMGEVYRAKDSRLNREVAIKVLP
                     EDLFEGEERKQRFEREAKLLATLNHPNIAAIYSFEEISGRHLLTMELAAGEGLDKKIS
                     AGPLPLEESLSFARQIAEALEAAHEKGIVHRDLKPANVVVSAEGRVKLLDFGLAKAFE
                     SEVGSSPEISHSPTLTARATAAGVILGTAAYMSPEQARGKPVDKRADVWAFGVVLFEM
                     LTGKRLFQGETVSDTLAAVLREPVDFGQLPPSTPPSVRVLLERCLERDPKQRLQAIGE
                     SRIALERTIAGSSGIRVVSSKETAPATRRGGASLVVGWALAAVFAAVAAILALRSRVP
                     EARVYRASIPAPEGTVFSVDTTQPGPPTLSPDGKKLAFAARGADAKVRLYVRALDATA
                     AQPLAGTEGAQYPFWSPDSRFVAFGADQKLKKIEASGGPPTVLCPTTDFPKGGAWSPK
                     GVIVFAPSSGGPLWAVSENGGEARQVTKLDAKRGDNSHRLPFFLPDGAHFLFLARLGG
                     ASPVEGNQILVGSVDGGEPKPILRSPAAAEYASGYLLFMRDAALMAQRFDPSRLSLLG
                     EPHPVAESVSIVRGAAKGTFSASQTGVLVAHIGDSIVLGADLEWVDRAGKTLAPLSEH
                     APFDEAVISPDGRNVAVSVLDFKFGTHDIWICDVARNLRTRFTFEPGEELAPRWAPDG
                     RSITYVAGRGAQQGLFRKQVEGTATEELLYASETVKVPSGFSPDGKLLAFAELGQDTN
                     FDIWILPLTGERKPYVFLKTTFSEGGAVFSPDGKWIAYQSNESGKSEVYVTPFPGPGR
                     KWQVSAQGGGYPAWRQGGREIVYQEMQSNKVFSVPVAFRGDTPDFGRAAELFVAPPPL
                     AGIAARFDSAADAKKFMVVRQGKQREAQALTLVVNWTAELEGKK"
     gene            10982..11407
                     /locus_tag="PLB01_10165"
     CDS             10982..11407
                     /locus_tag="PLB01_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HQQ77708.1"
                     /translation="MSPMTLAVFESSLKRCEARPDFFDVFYGRFLGSSPEVAVKFAGT
                     NFTRQKELLRVSLHHLLLVARDPKQGPDPYLEDIAVKHGAGHLAIGAHLYDLWLDSLL
                     ETVRECDPECFPEVEKAWEEVMMVGIHYLCANYEGRHRR"
     gene            11451..>11992
                     /locus_tag="PLB01_10170"
     CDS             11451..>11992
                     /locus_tag="PLB01_10170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ77709.1"
                     /translation="MTLEAGIRLGPYEVLSPLGAGGMGEVYRARDPRLGRDVAVKVLP
                     EDFLEGEENRARFEREAKLLAGLSHPNIAAVYSFEEVPGPSPSSSRHILVMELIEGET
                     LAARLAKGAIALDQLLPIGMQIADALDKAHRQGIVHRDLKPGNIMLTKGGAKLLDFGL
                     AKVLAPAGHTSGATSLPTMAS"
BASE COUNT         1863 a         4083 c         4209 g         1837 t
ORIGIN      
        1 acgcatcgcg aaagcgcgct tcgcaccctc cggccaggac gtcatctacg acgcgagctg
       61 gcagggcgag ccgtggcagc tcttcgcgac gccgatcgtc gaccccaagg agaggtccct
      121 cggccagccc gacgtgcagc tgctcgacct ttcccgggct ggcgagctcg tgctgcgcct
      181 caagagcaag gtcgcggaca cgtggcagtt cgagggcacg ctcgcgaggt cggctctcgg
      241 cggcgacctc gctccgaagg cgattctcgg cggcgttctt caggcggcgt gggcgccgaa
      301 gtccgaattc gcagtcgtgc gcgcgacggg cgggcgatcc ctcctcgact atccgccggg
      361 caaggtcagg gtcgagtcgc cggggtggat cggagacctt cggttctcgc cggacggctc
      421 gatgctcgcg tacgtcgacc acgcgaacgc gggcgacgac gcggggcgcg tcgccgtcct
      481 cgacgtcgcg gcggggaaga cccgcttcct ctcgaaggag tggggcacgc tccgcggcct
      541 cgcgtggaag ggcaaggaga tctggttcac ggcgggcacg cacatcctga atcgcgcgct
      601 ctacgccgtg gacctctcgg gcaaggagcg cggcatcttc acgcaggccg gcggcctcca
      661 tctccacgac gtcgcatcgg acggccgcgt tctcatgagc cgcgaagaga tgcgcggcgg
      721 gctcctctcg cagaccgagg gagtgctgca gccgctcgac ctttcctggc ggacgtggag
      781 ctaccttctc gacacctccg ccgacggaaa gacgctgctt ttcgaggagg aagagggtga
      841 tcccgccggc tacgatctgt tcctcaggcc gacgtcggga ggtcacgcga cgccgctcgg
      901 gaagagcgcg ggctacggcg cgctctcgcc ggacgggacg ctcgccgtgg cgtggctcgc
      961 gaagccggct ccgcggtggg tcctttatcc gaccggaacg ggcgctccgc gtgagatccc
     1021 ggcccacgga atccgcagca tcaagtccgt ttcgttctcg aaggacggcc ggagcctgat
     1081 cttcatcggg ccggagggcg aaaaggagga tcgggtctgg tcgctggctc tcgacggcaa
     1141 ggagcccaag cccctcactc tcgaaggcgt cacgggcctc gagacgtggc cgctgctgag
     1201 tccggacggc aagagctttc tcgcgcgcga gaagggcggc tggtcgatct tcgacctcgc
     1261 gaagccggac gcgccggcga ggcgcgtcgt cggcctcgag gacggcgagg tcctgacgca
     1321 gtggcgcgcc gacggccggt tcttcgtcac gcggcccggc gcctttcccg tcgacgtgtg
     1381 gatgctggac cccgcgaccg gaaagcgcca gccgtggaag acggtcagcc cggcgggcta
     1441 cgtcggcgcc gactgctcga cgatccggtt cagcgaagac ggcaagcgca tggcggcgcg
     1501 gtacatcctc cagcgctcga ccctctacgt cgtcgagggt ctccgctagc gatcaggcgg
     1561 cgcgcacggg cgccgggacg acctcgggga gcgccgcgag aaacgcctcg acctcctcgc
     1621 gttttccgat tgtcacgcgc agccagttcg ggagggaggg gaacttcctc ccgacgagta
     1681 agtttctcgc ccggaacgcc tggatgaaag gcgcgacgtc gccgcccacg tcgatcatca
     1741 cgaaattcgc ctcggaaggc atcgtccggt agccgagctt cgtgagctcc gacacgagcc
     1801 acttccgcgt gtcgttgagg agcttcctct gccgcgggac gaggtcggga tcggcaaggc
     1861 tcgcggccgc ggccgccaga actcccgcgt tggcgttgct ccacgaggcg tagggcgcca
     1921 tcgcggcgat cgtctccttc gacgacacgg cgtacccgag cctcatgccg gccatcccgt
     1981 agatcttcga gaacgttcgc gcgacgacga cgttcgggcg cttcgcgatg agctcgaggc
     2041 tggagcggta gcgcgggtcc tccacgaagt ggtggtaggc ctcgtccacg aggatcgccg
     2101 ccgaggccgg gaccgcggac gcgaaggccg ccatctcgtc gcccgtcacg atcgtcgcgg
     2161 tcggattgtt cgggttgcag acgtagacga ggccggtcga agcgtcgcac gcggcggcca
     2221 tcttcgcgag gtcgtgccgg aagtcgggcg tgagggcgac cttgatcccg tcggccttga
     2281 cgaccttcgc gtacgcgagc acggcctcga acgtcggctc ggccgcgacg accttcttac
     2341 ccgggccgct gaacgcctcg tcggccatct ggaggatctc ggaggagcca cagccgagga
     2401 ggacctgctc gggggcgacg ccgtgatgct tcgcgatcgc ctcgaccgcg gcgttctcga
     2461 gcgcgtcggg gtagcggttc gtggcggcct tcgcggccgc ctcgagcgcc ttcggcgacg
     2521 gcccgtacgg gttctcgttc gagctcagga cgacggcgtc ggccgggcgc ggacgcttcg
     2581 tcgcagccgc ggcgacgcgg cccacgaggg gagtgtcgag aaacgcaaca cctgcggcgg
     2641 cgccgaggga tccggcgaac gcccggcgag acatcggaac ggtcatgagc ggcctcctcc
     2701 gaagaagatg cgacgcatca tctcgcatcg ggcgcgaacc gcgcacatgc gactctttcg
     2761 cggctagaat ccggtcaaag atatgacgat cgcttctgga acccgcctcg ggccgtacga
     2821 ggtcctgtcg ctcctcggcg ccgggggcat gggggaggtc taccgggcca aggaccctcg
     2881 cctcaaccgg gacgtcgcgg tcaaggtcct gccggaagat ctcttcgaag gtgaagagag
     2941 aagacagcgt ttcgagcgcg aggcgaagct cctcgccacg ctgaaccatc cgaacatcgc
     3001 ggcgatctat tcgttcgagg aaatctccgg ccggcacctc ctcacgatgg agctcgccgc
     3061 cggagaagcg ctcgacaaga agatctcctc gggccctctt ccactcgaag aatcgctttc
     3121 tctcgcccgg cagatcgccg aggcgctgga ggcggcccac gagaggggca ttgtccaccg
     3181 ggacctcaag cccgcgaacg tcgtcgtgtc gtcggagggg aaggtcaagc tcctcgactt
     3241 cggcctcgcg aaggcgttcg agaacgagaa cgggtcgtcg ccggagatct cgctctcgcc
     3301 gacgctgacg gcgcgggcga cggcggccgg cgtgatcctc gggacggcgg cgtacatgtc
     3361 gcccgagcag gcgcggggca agcccgtcga caagcgggcg gacgtctggg cgttcggcgt
     3421 ggttctcttc gagatgctca cgggcaagcg gctcttccag ggcgagacgg tcagcgacac
     3481 gctcgcggcc gtcctgcgcg acccggtgga tttcgggaag ctgccgccct cgacgccgcc
     3541 gtccgtgcgg gtgctcctcg agcgttgcct cgagcgcgac ccgaagcagc ggctccaggc
     3601 gatcggcgaa tcgcgcatcg cgctcgaacg gacgatcgcc gggagctcgg gagccatgaa
     3661 ggccgcgacg gattcgggtt ccgccgtcgc cgtcgcggct ccgtctcccg agcgtcgcgc
     3721 gttcccgtgg gcgatcggcg ccgcggtcgg cgcgctcgcc gtgtttgcgc tctgggcccc
     3781 gtggaagaag gcgccgcggc cggcgcccgt ccgcctcagc agcgagctgg gggcggacgc
     3841 ctctctcgtg acggacctcg gcccggccgc cgtgctttcg ccggacggga cgctcctcgc
     3901 attcgtcgcc cagaagaccg gcggcgggtc ggcgcagatc tgggtgcgcc ggctcgacca
     3961 gcttcccgcc gcgccgctcg cggggaccga gggtgcgcgg gagcccttct tctcgcccga
     4021 cggccagtgg atcggcttcc tcgcggacgg gaagctcaag aaggtttccg tgaccggcgg
     4081 cgcggccgtc gttctctgcg acgctccgaa cctccgtggc gctttctgga cggaggacga
     4141 gacgatcgtg ttccttccga actccgggcc cagcgtagcc ctggtgaagg tcccggccgc
     4201 gggcgggaag cccgagccgt tcacgaagct cgaggaggga gaggtcaccc agcgctggcc
     4261 ccagatcctt ccgggaggca aggccatcct ctatacgggg cacgcgacga cgtcctcggg
     4321 gttcgaggac gcgaacatcg tcgtgcagcc tctgccgaag gggccgcgga agatcctcca
     4381 acgcggcgga tatcacggcc ggtacctccc gagcgggcac gtcgtctacg tccacgagtc
     4441 gactctcttc gcggctccgt tcgatctcgg gcggctcgag ctgaccgggc cgccggtgcc
     4501 cgtcgtcgag ggcatcgcct cgacgcccaa cagcgcgggc tcgaacttcg cgttctcgaa
     4561 cggcggcacg ctcgtgtacc tgccggggaa gagcgtgggt gcggcggcgc cgatccactg
     4621 gatggaccgc gagggcaaga cgtcgccgct ccgctccacg gcctccgcct ggaacgacat
     4681 ccgcttctcg ccggacggcc agaaggtcgc gatggacgtg accgacggcg ggcagaccga
     4741 cgtctgggtg tacgaatggg cgcgggacac gatgtcccgc ttcacgttcg acctcgccga
     4801 ggattcgcat gcggcgtggt cgccggacgg caagcgcatc gcattcgcct cggcgcgagc
     4861 cgacaaggcc gtgacgaacc tctactggca gcgatccgac gggaccggag acgtccagcg
     4921 gctcaccgag agcaaggtcc ggcagatctc cacgtccttc catcccgggg ggcgctttct
     4981 ggcgttcatg gagcaggatc ccaagacgaa ctgggacatc gtgatcctcc cgatcgaggg
     5041 agacgaggcc tcgggctgga ggccggggaa gcccaccgtg ttcctcaact cgccgttcgt
     5101 cgaggtctgg ccggaattct cgccggacgg aagatggctc gcctacatgt cgaacgagtc
     5161 ggggcggttc gaggtctacg tccgcccctt cccgggcccg ggagggaagt ggcagatctc
     5221 gacggagggc ggcggattcc caacctggtc gcgggcgagc cgcgagctct tcttcggaca
     5281 ggacgggaag atcatggccg ttccctactc ggcggacgcg gcttcgttcc gcgccgagaa
     5341 gccgcagccc ctgagcggag ggcgatacac caccaccggc ccgtttcgcg cattcgacct
     5401 gcacccggac ggaaagcgct tcgcgatcct gaagccgccg gatgcccagt ccgacgtccg
     5461 gcacgacaag gtcgtcttcg tcttcaattt cttcgacgag ctcaagcgcg tcgcggggac
     5521 ggggaagaag tgacgatcgc ggcgggaacg aagctcgggc cctacgagat cgtctcgccc
     5581 ctcggggccg gggggatggg tgaggtctac cgggcaaagg acacccgcgt cgacagggtt
     5641 gtcgcgctga aggtgctgcc ggaagagttc ttcgaagatg aagagcgaag ggcgcggttc
     5701 gggcgagagg cgaagctgct cgcgagcctg agcaatccgg ggatcgccgt tctctactca
     5761 ttcgaagaaa tccccggttc ttcttctccc tcgtcttcgc gtcacctgct cgcgatggaa
     5821 ttggtcgagg gggaggacct tgcggcgcgc attgcctccg gctctctctc actcgaagaa
     5881 tccctctccc tcgcccggca gatcgccgag gcgctcgagg cggcgcacga gaagggcatc
     5941 gtccaccgcg atctgaagcc cgcgaacgtg aagatcacgc ccgagggtcg cgtgaagctg
     6001 ctcgacttcg gcctcgcgaa gatcttcgag agcgaggcga cgttgtcgaa cccgtcgatc
     6061 tcgtactcgc cgacgctgac ggcccgcggc acggcggcgg gcgtgatcct cgggacggcg
     6121 gcgtacatgt cgcccgagca ggcacgcggc aagccggtgg acaagcggac cgacgtctgg
     6181 gccttcgggt gcgtgctcta cgagatgctc gcgggaaaga aggcgttcga aggcgagacc
     6241 gtgagcgaca cgctcgcggc gatcctgcgc ggagagccgg actggagtgc gcttcgcgcg
     6301 ccggtttcct cgaaggtcaa tgagctgctg cgcaggtgcc tccagcgcga ccccaagcag
     6361 cgcctccgcg acatcggaga tgcgcgcatc gccctcgagg aggagacggc cgtgtcggcg
     6421 agccgagccg gccaattacc cttcgaagag aatcctgcgt tgccgagtcc cgcggtagca
     6481 agcgtggagc gagcgggtcg tgagagaggg agcaaaaaga atctctcggt ctttcttccc
     6541 tgggtgcttg cggccgcgtt cgcggccgcg gcaggggcgc tcgcgctgcg cgctcgcgcc
     6601 cccgaggcgc gcgtgtaccg ttccgtcctt ccgccgccgg aaggaaccaa cttctggctg
     6661 gaggcgaacg gcccggggcc cgtcgtcgtg tccccggacg gccgccaggt cgcgttcacg
     6721 gcggccgacg cggccggcaa ggtcaacctc tacgtgcgcg cgctcgactc ccgcgaggcc
     6781 cggatgctct cggggaccga agcggccatg taccccttct ggtcgccgga cggcggctca
     6841 ctcgggttct tcacaaccgg gaagctgaag acgatcgagg ccacgggcgg ttcgccgcag
     6901 acgatctgcg gcgcgcccga gggaaaaggc gggacctgga gcccggcggg tgtgatcgtc
     6961 ttcacccccg gcccgacctc gcctctcttc agggtctccg aaaagggagg cgagacgacg
     7021 gcgatcacga agatcgacgc gtcccgcggg gacgactcgc accgacaccc ccggttcctc
     7081 ccggacggaa agcactttct ctacgtcgcg cgctcgacga agaccgggtc cgaggggcac
     7141 tacgtcgtcg cggcgtccct cgacggagga gcggagaagg tgctcctgcg gtcgcctgct
     7201 gcggcggtgt acgcgtcggg ccacctcctc tacctgcgcg agacgacgct gatgtcgcgg
     7261 cccttcgacg ccgcgcgcct ggcgttcacg ggcgaggcct cgccggtcgc cgagaagatc
     7321 ctcatgccgg cggtcggcac ggcgctcggg gtcttctcgg cctcgcagaa cggcgttctg
     7381 gcctaccaga ccgcgcgcgg cgagacgacc tcgcggctcc agtggttcac ccgggacgga
     7441 aagccggacg ggacgctcgg cgaacccgcc gacttcgggc aggcgcttct ctcgccggat
     7501 gggaagctgg ccgcggccac catccgggac caggcgaccg ggacgtacga catctggatc
     7561 ttcgacgtcg cccgcggggt gcgcacgcgc ttcacgttcg atcccgccga ggacgtgggg
     7621 ccgctctggt ccccggacgg ctccgcgctg atcttcgcgt cgaaccgcaa ggggcactac
     7681 gatctctatc gcaaggccct ggacggctcc tcggaggagg aggccctgtt cacgtccgac
     7741 gccgacaagg gccccgtctc ctgggcgccc ggcgggcgca ccctcgtctt cgacgaggcg
     7801 ggcaaggacg ccggagtcga ggtcaagacg ctcgcgctcg acggggcccg gaagccggag
     7861 cccctgtcga agacgaagtt cagcgaggtg aattcccctc tctccccgga cggccggtgg
     7921 ttgccctaca gcaccgacga gtcgggccgg tgggaggtct acgtcacctc gttcccgcgc
     7981 gcgggacgaa agtggcaggt ctcgaccgac ggaggggcct atgcgttctg gagcgcggac
     8041 ggcaaggaaa tcctctacca tgacctcagc gggatgatct gggcggtccc cgtttccgcc
     8101 cggggggaaa ctttcgagat cggccagggc cggcggctct ttcgcgcccc gggccccaac
     8161 cccacggctc cttcgttctc cgcgacatcc gatcaccagc gcttcctcgt cgtgggcgag
     8221 gggcagaagc ccaacgcgct cgtggatctc gtcgtgaact ggacgattgc gaagggctcg
     8281 cgatgacgat cgccgcgggc acccggctcg gcgcttacga ggtcctcggg ctcctcgggg
     8341 ccgggggcat gggggaggtc taccgggcga aggactcccg cctcaaccgg gaagtcgcga
     8401 tcaaggtcct gccggaagac ctctttgaag gtgaagagag gaagcagcgg ttcgagcggg
     8461 aagcgaagct gctcgccacg ctcaaccacc cgaacatcgc ggcgatctac tccttcgagg
     8521 agatctccgg ccggcacctc ctgacgatgg aactcgccgc cggagaaggg ctcgacaaga
     8581 agatctccgc tgggcctctt cccctcgaag aatccctgtc tttcgcccgg cagatcgccg
     8641 aggccctgga ggccgcacac gagaagggga tcgtccaccg cgacctcaag cccgcgaacg
     8701 tcgtcgtctc ggccgagggt cgggtcaagc tcctcgactt cggtctcgcg aaggcgttcg
     8761 agagcgaggt cggctcgtcg ccggagatct cgcactcgcc gacgctcacg gcgcgcgcga
     8821 cggccgcggg cgtgatcctc gggacggcgg cgtacatgtc gcccgagcag gcgcggggca
     8881 agccggtcga caagcgggcg gacgtctggg cattcggcgt cgtcctcttc gagatgctca
     8941 cgggcaagcg gctgttccag ggcgagacgg tgagcgacac gctcgcggcc gtcctgcgcg
     9001 agcccgtgga cttcgggcag ctgccgccgt cgacgccgcc gtccgtgcgc gtgctcctcg
     9061 agcggtgcct cgagcgcgac ccgaaacagc gcctgcaggc gatcggcgag tcccggatcg
     9121 cgctcgaaag gaccattgcg gggagctcgg gcattcgtgt cgtgtcctcg aaggagacgg
     9181 cgccggcgac acggcggggc ggagcctctc tcgtcgttgg gtgggcgctt gccgccgtct
     9241 tcgcggccgt ggccgcgatt ctcgcgctgc gctcgcgggt gcccgaagcg cgcgtctatc
     9301 gcgcctcgat ccccgcgccc gaaggcacgg tcttttccgt cgacacgacg cagccgggcc
     9361 cgccgacgct ttctcccgac gggaagaaac tcgccttcgc ggcgcgcggc gcggacgcga
     9421 aggtgcgcct ctacgtccgc gccctcgacg cgacggccgc ccagcccctc gcggggaccg
     9481 agggagcgca gtacccgttc tggtcgccgg acagccggtt cgtcgccttc ggtgcggacc
     9541 agaagctcaa gaagatcgag gcttcgggcg gccccccgac ggtcttgtgt ccgacgacgg
     9601 acttcccgaa gggcggcgca tggagcccga agggcgtcat cgtgttcgcg ccgtcgtcgg
     9661 gcggcccgct ctgggccgtc tccgagaacg gcggcgaggc gcggcaggtc acgaaactgg
     9721 acgccaagcg gggcgacaac tcgcaccggc tgccgttctt cctcccggac ggcgcgcact
     9781 tcctgttcct cgcccggctg ggcggcgcct cgcccgtcga ggggaatcag atcctcgtcg
     9841 gatccgtcga cggcggcgag ccaaagccga tccttcgttc gccggccgcg gccgagtacg
     9901 cctcgggcta cctgctcttc atgcgcgacg ccgcgctcat ggcgcagcgg ttcgacccgt
     9961 cgcgcctgag tctgctcggc gagccgcatc ccgtggccga gagcgtgagc atcgtccgcg
    10021 gcgcggcgaa gggcaccttc tccgcctcgc agaccggcgt cctcgtcgcg cacatcgggg
    10081 actccatcgt tctcggcgcc gacctcgagt gggtcgaccg ggccgggaag acgctcgccc
    10141 cgctgtccga gcatgcgccc ttcgacgagg ccgtgatctc gccggacggg cgcaacgtcg
    10201 ccgtgtcggt actcgatttc aagttcggaa cgcacgacat ctggatctgc gacgtcgcgc
    10261 ggaatctccg gacgcgcttc acgttcgagc cgggcgagga gctcgcgccg cggtgggcgc
    10321 cggacgggcg gtcgatcacg tacgtcgcgg gccgcggcgc gcagcagggg ctgttccgca
    10381 agcaggtcga gggcaccgcg acggaggagc tgctctacgc ctcggaaacc gtcaaggtcc
    10441 cgtcgggctt ctcgcccgac ggaaagctcc tcgccttcgc cgagctcggc caggacacga
    10501 acttcgacat ctggatcctc cctctgaccg gcgagaggaa gccctacgta ttcctcaaga
    10561 ccacgttctc cgaaggcggc gccgtcttct cgccggacgg aaagtggatc gcgtaccagt
    10621 cgaacgagtc gggcaagtcc gaggtctacg tcacgccctt cccggggccc ggccggaagt
    10681 ggcaggtctc ggcgcagggg ggcgggtacc ccgcgtggcg acagggcggc cgcgagatcg
    10741 tctaccagga gatgcagagc aacaaggtgt tctcggtacc cgtggcgttc cggggcgaca
    10801 cgccggactt cgggcgtgcg gccgagctct tcgtcgcgcc accgcccctc gcgggcatcg
    10861 cggcccgctt cgactcggcg gcggacgcga agaagttcat ggtcgttcgc cagggcaagc
    10921 agcgcgaagc gcaggcgctc acgctcgtcg tgaactggac ggcggagctg gaggggaaga
    10981 aatgagcccg atgacgctcg ccgtcttcga atccagcctc aagcgctgcg aggcgcggcc
    11041 cgacttcttc gacgtgttct acgggaggtt cctgggctcg tcgcccgagg tcgccgtgaa
    11101 gttcgccggg acgaacttca cgcggcagaa ggagctcctg cgcgtctcac tgcaccatct
    11161 cctcctcgtc gcgcgcgatc ccaagcaggg gccggacccg tacctcgagg acatcgccgt
    11221 caagcacgga gccggccacc tcgcgatcgg cgcccacctg tacgacctgt ggctggacag
    11281 cctcctcgag accgttcgcg aatgcgaccc cgagtgtttc ccggaagtgg agaaggcgtg
    11341 ggaggaggtc atgatggtcg ggatccatta cctgtgcgcg aactacgagg ggcggcaccg
    11401 ccggtgacgg acgcgaagca gcgggtcgca gaaaggatcg aggaggaatc gtgacgctcg
    11461 aggccggaat ccgcctgggc ccctacgagg tcctgtcccc gctcggcgcg ggaggcatgg
    11521 gagaagtcta tcgggcacgg gaccctcgcc tcggacgcga cgtcgcggtg aaggtgctgc
    11581 ctgaagattt cctcgaaggt gaagagaaca gggcgcgctt cgaacgggaa gcgaagctgc
    11641 tcgcggggct gagccatccg aacatcgcgg cggtctattc attcgaagaa gtccccggtc
    11701 cctctccttc ttcttcgcgg cacatcctcg tcatggagct catcgagggc gagacgctcg
    11761 ccgcacgcct cgcgaagggg gcgatcgctc tcgaccagct cctgccgatc gggatgcaga
    11821 tcgcggacgc cctcgacaag gcccaccggc aggggatcgt ccaccgggac ctcaagcccg
    11881 gcaacatcat gctcacgaag gggggcgcca agcttctcga cttcggcctc gcgaaggtcc
    11941 tcgcacccgc ggggcacacg tccggcgcca cgtcgcttcc gacgatggcc tc
//
LOCUS       DAOMFY010000105        11911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1658_length_11911_cov_7.075995, whole genome shotgun sequence.
ACCESSION   DAOMFY010000105 DAOMFY010000000
VERSION     DAOMFY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_11911_cov_7.075995"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..6223
                     /locus_tag="PLB01_10175"
     CDS             125..6223
                     /locus_tag="PLB01_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HQQ77710.1"
                     /translation="MKHARRISLFAAAVLLAAGSARVAFTFAGARVTGPATWKDVDRL
                     VSEQKFEEAASAVGKIREAARAAGDESDEAKALVREVQLRTALHGYETSVRFLKDQPW
                     PKGLLPRTTLRLFYAQSLVTYAQAYGWEIGKREKVSSTGAVDLKAWTRDEIQAEAVRA
                     YTEVWKDREALGRVAVNAVAEFVEPNNYPKEIRGTLRDAAAYLFTALLADTSGWRPEQ
                     ANGVFALDARLLVAGDPGVSRLVKLEDSAVHPLLKLGAILDDLEAWHAGAGEKEAALE
                     ARLERLRRLDAALTDPGDKAAIRKDLAAKLPAYAGVPWFSMGKAQLSDFTRAEDKPGN
                     FVRARALALEAIQAYPKSLGAQRGRWIVGEIEARDWSIAAMAADGAGRRSIEVTHRNL
                     SALYFRAYAVDLEARIGRAKDYNLLPQGRELDELIAREKPVASFEVALPATTDYKAHR
                     TFVTPPALGRGLHVVVASADPGFNPDKGPLHAVGMIETDLVLAVRQTESGGVEARAVT
                     GGTGRPAPGTVVSLWQYDWQKGHRKIADAKAGPEGLVSFEASPDSRNGTFLVARRGDD
                     VALDASFMSLWTRGKSGEISSALVYTDRSIYRPLQKVLWKAVVYRGRADQAKFRVAPA
                     TTVSITLVDANGQAVESKTVTTNAYGSAAGEFALPAGRALGAWNVRCDWGGAASIRVE
                     EYKRPTFEATLAEAKEALRLNRPATFRGEARYYFGLPVANGTVAWRATREPEYPWWWW
                     WGWRGARAGGVETVATGNAALGADGTFAITFTPKADERQSKEITYRYRVSADVTDEGG
                     ETRSADRSFRLGFVGVKARLERERGFFLEGNRAGATFVRSDLDGAPRAGRGAWRLLRL
                     VSPEKAVLPAELPSKKSEIGASSRDNGAALAGDIMTPGDLLRPRWSTGYSVDEWLHSW
                     KDGAEQASGSLVHDAKGEAKLELPALSSGAYRLRYSTVDDFGAKYEMAQDFLVAGGKT
                     APVLPVVLLAESSSVPVGGVARFLVASGLRDQTMFLDVARAGRPIERRVLKAGTDPVV
                     IERTITEDDRGGLGVTLTAVRDHQFLQQMQSVFVPWDDRELKVSFATFRDRLRPGQTE
                     TWRVTVKGATPDHPLREAAELLAYMYDRSLDSFTPHHPQSPLFLLPNRATPLVARATL
                     GAAPQLWLTSSGFPPPPMPPILVGDRLKFESGYGIGGPGVRGRGMGRAYNEAMPAAPA
                     AMALKSAARQEMAVGAVAESKMADAKDGRSDETDSLSKKKENGAMREDAAGDASALRS
                     NFSETAFWQPQLLTGTDGSASIEFTVPDSVTSWRVFVHAVTKDLRGGSVDAETKTVKD
                     LMVRPYVPRFLREGDRAEIKVVVNNASAKPLSGRLAFEITDPATGKSLLPAFGLAGEA
                     ASRAFTVPAGGGTNLSFPVTAPKGLTTVAFKAVATAGDLSDGEVRPLPVLPGRMHLVQ
                     SRFVTLKNKDRKTLRFDDLARNDDPSLVNEQMVVTVDAQLFYTVLQALPYLVNYPYEC
                     TEQTLNRFLSTGIVSSVYRDYPAVAKMAAEMSKRTTPLESWDATDPNRKMALEETPWL
                     ATAQGNRPGENGSINVLDPRVAKANQESALARLKKAQTSIGAFPWFPGGPPSPYMTLY
                     ILHGFAKGLEFGVDVPKDVTTRAWGYVAQHWRSDWRACMVHDGCWEFVTFLNYVLSTY
                     PDVSWSGGAFTDAERKEMLDFSFRHWRQHAPYLKGYLALTLKRSGRAADAKLVWDSVM
                     DSAKSNDEQGTFWAPEDRSWLWYNDTIETQAFALRTLLELDPANGKMDGLVQWLLLNK
                     KLSQWKSTRATAEVIYSLVSYLKKQGALGVPEDATVTVGVQKVAFTFDPEKYTGKNNR
                     IVIPGEKVDPKTTSTVVVEKEGKGFVFASAAWHFSTERLPAEDRGDFFAVSRKYFRRE
                     NTGKEFTLTPLAEGAVIRPGDQIEVQISLRTKHEAEYVHLRDPRAAGLEPENVLSRWK
                     WDLGIAWYEETRDSATNFFFERLPVGEYTFKYRLRANMAGTFRVGPATVQSMYAPEFN
                     AYSAGAVLRVAGGSPP"
     gene            complement(6195..6680)
                     /locus_tag="PLB01_10180"
     CDS             complement(6195..6680)
                     /locus_tag="PLB01_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ77711.1"
                     /translation="MYTVPTLIRKADGAADVATARALFEEYQQSLGFSLCFQNFDAEL
                     AGLPGAYAPPEGRLLLAFAGDEPAGCIALRKIGEEICEMKRLWVRPAFRGSGLGRRLA
                     ESLMAEARAAGYRAVRLDTLPSMAAAQALYLSLGFLDIPPYNDHPIEGTRFMEAILPP
                     P"
     gene            complement(6646..6867)
                     /locus_tag="PLB01_10185"
     CDS             complement(6646..6867)
                     /locus_tag="PLB01_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77712.1"
                     /translation="MSPAFEAYLAKIYTDEAERAAFLTDPRGRAAAARLSPAEVDALA
                     AIDRDGLVLAAQSFQRKRDVHRAHSHQKG"
     gene            complement(6864..7721)
                     /locus_tag="PLB01_10190"
     CDS             complement(6864..7721)
                     /locus_tag="PLB01_10190"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HQQ77713.1"
                     /translation="MKDFVGLGWRPAIADRIFAAADRLDVVEVIAEDWMKRPAREVRA
                     LRTLGAHVPVVLHGVSLGLASSAPVHPKRLDAFARVVELAEPVSWSEHLAFVRGGGIE
                     IGHLAAPPRCAATIEGTARNVARARAVVGSAPLLENVATLIDPPGSDRDEATFVADVL
                     EACGTDLLLDLHNLHANARNFGFEEEDFLRRIPTQRVRAIHLAGGRSIGVPGQEGETR
                     ILDDHLHAVPDVVFTLLESVAAAVPRPLTVILERDGAFPLFEEILSELSRARASLAAG
                     RARRAEAAA"
     gene            complement(7723..8592)
                     /locus_tag="PLB01_10195"
     CDS             complement(7723..8592)
                     /locus_tag="PLB01_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HQQ77714.1"
                     /translation="MNPFALTGPQFLLFYVAFAAATVWVIARMRKSAESGDPMRVSLT
                     DPYAIAYLRGGPNETLRVASVALVDRGLLVPSGTTLQAKKGAHAQNALEAVLLEKFRS
                     FGEATSIFNDKSLEAATVPVRNSLADLGLLPGPAENAARTRVFVLGLGVLWAVSGARL
                     LQAFSRGRHNVAFLVILSIFAAVAALAAAFPRRTAKGDRLLADIRTLFSGLKGRAASL
                     RPHAGSAEVALLAAVFGVGLLPAAGFEWAKKLFPKASASGTASSSCGSSCGSSCGSSC
                     GGGCGGGCGGCGG"
     gene            complement(8589..9311)
                     /locus_tag="PLB01_10200"
     CDS             complement(8589..9311)
                     /locus_tag="PLB01_10200"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQQ77715.1"
                     /translation="MELPEPAARALDEFLAAARTSLGGDLSSAVLFGSAAEGALRPES
                     DVNVVLVLKALPERSVENLRGALRLAQAAARLEVMFLLESEIAEAAEAFAVKFADIAR
                     RRRVLFGTDPFAGLKVPEDALRRRLSQSLLNLMLRLREAWALHGASDDEAARSVSNFT
                     GPLRAAAASLLSLEGRAVPSPKEALRQAAADLPGEGWPALVESLSAVREQRPLGPGEA
                     ARILGRLPELASLLRARARHAS"
     gene            complement(9341..11362)
                     /locus_tag="PLB01_10205"
     CDS             complement(9341..11362)
                     /locus_tag="PLB01_10205"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HQQ77716.1"
                     /translation="MNRRFARPAASAAFMLALAAALPAHSQKSSPPKSPVSSPKQKSP
                     SAFATLEADYVRAFLNRFPVVATYLGASGLDPAYAALDGKLRDYAPAALLAERDEWRG
                     FEARLAKVDRKKLTESDRIDAAVMKAHLAFLIHNHERRIHEKALDVFLEEPLRGIEWT
                     LQGMEPAAGGARGTKAEWDAIASRTAAVPAYLKAALANVRRGASGDAIPDRRLVAVSV
                     DAAAATAAYFEKTLPERSKEAGPALDEAGRAALLSASAAAAAAFRDFRKGLVDLFYQP
                     DGKTLKPAFDRDRYAAGEIEYTWALKNNLGISATPRELHARGKTAVAATLSEMTALAR
                     RIALSRLWADASLPAVVAKLEDDIPKTDAEMLASYRDACQRLVAYGRKTGMFDLPADY
                     RLDVLYTPPPLRDSIDAAYYPAPQFKHGGVGQFYVTPTGNDPAALRDHNRPAIASTAA
                     HEGFPGHDWYYQFTRARSASIPVIRWLLPGGVEDSASMWEDALSSEGWALYTEQLIGE
                     PRDGYPEGFYTPEERLIQLQNQLLRDARVVVDTGLHCGFMTFDEAVAYLARNVRLVQG
                     PVSTDPKVNPSAAERAAVEGMKKAAFRYSKWPTQAITYHLGKAEILSLREEYRRIEGA
                     AFSEKRFHEEFLSEGQIPPALFRERLLARARARTASTGAPNAADIGISR"
     gene            complement(11404..11793)
                     /locus_tag="PLB01_10210"
     CDS             complement(11404..11793)
                     /locus_tag="PLB01_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77717.1"
                     /translation="MSQEILEGLAVARRAPGRYLGAALAAFAFLALHLAALFVTARGL
                     GLPLHPATLAVALLASITTAAVIPSPAGTFGPYESGFAAAAALDGLSLATGAVLGGLL
                     HLLTTLAAGAVGLAFFLGRLRENPPRD"
BASE COUNT         1835 a         4019 c         4394 g         1663 t
ORIGIN      
        1 gggcgccgac ggtgccgggg aaggacgaga gcgccgccgg ctcgaagccg tcgcggcgtt
       61 ccgtctgacg cgctgaccgg gaaccttcac gggtccccgg ggtcatagac tcaccggaga
      121 cgccatgaaa cacgcacgcc ggatctccct cttcgccgcc gccgtccttc tcgccgccgg
      181 gtccgcccgc gtggccttca cgttcgcggg ggcccgcgtg acgggcccgg ccacgtggaa
      241 ggacgtggac cggctcgtct ccgagcagaa gttcgaggag gccgcttcgg ccgtcgggaa
      301 gatccgcgag gcggcgcgcg cggcgggcga cgagtcggac gaggcgaagg ccctcgtccg
      361 ggaggtccag ctccggaccg cgctccacgg ctacgagacg tcggtccggt tcctgaaaga
      421 ccagccctgg ccgaagggcc tcctgccgag gacgacgctg cgcctcttct acgcgcagtc
      481 cctcgtcacg tacgcgcagg cgtacgggtg ggagatcggc aagcgcgaga aggtctcctc
      541 gaccggcgcg gtggacctca aggcgtggac gcgggacgag atccaggccg aggccgttcg
      601 cgcgtacacg gaggtctgga aggaccgcga ggcgctcggg cgcgtcgccg tgaacgccgt
      661 cgccgagttc gtcgagccga acaactaccc gaaggagatc cgcgggacgc tgcgcgacgc
      721 cgcggcgtac ttgttcacgg ccctgctggc ggacacgtcc ggctggcggc cggagcaggc
      781 gaacggcgtc ttcgcgctcg acgcgaggct cctcgtcgcc ggcgaccccg gcgtttcgag
      841 gctcgtgaag ctcgaagact cggccgtcca cccgctgctc aagctcggcg cgatcctcga
      901 cgacctcgag gcgtggcacg cgggcgcggg ggagaaagag gccgcgctcg aggcgcgcct
      961 cgagcgcctg cgccggctgg acgcggcgct gacggacccg ggcgacaagg ccgccatccg
     1021 gaaggacctc gccgcgaagc tcccggcgta cgcgggcgtc ccgtggttct cgatgggaaa
     1081 ggcgcagctc tcggacttca cgcgcgccga ggacaagccg ggtaacttcg tgcgggcgcg
     1141 cgccctcgcg ctcgaggcca tccaggcata cccgaaatcc ctcggtgcgc aacgcggccg
     1201 ctggatcgtc ggcgaaatcg aggcgcgcga ctggtcgatt gcggccatgg cggccgacgg
     1261 cgccgggcgc cgctcgatcg aggtcacgca ccgcaacctc tcggccctct acttccgcgc
     1321 gtacgccgtc gacctcgagg ctcggatcgg ccgggcgaag gactacaacc tcctgccgca
     1381 gggccgcgag ctggacgagc tgatcgcgcg ggagaagccc gtggcgtcgt tcgaagtcgc
     1441 cttgccggcg accacggact acaaggcgca ccgcacgttc gtgacgccgc cggcgctcgg
     1501 ccggggcctg cacgtcgtcg tcgcgtccgc cgacccgggg ttcaatccgg acaagggacc
     1561 gctgcacgcc gtcggaatga tcgagaccga tctcgtcctc gccgtgaggc agacggagtc
     1621 gggcggcgtc gaggcgcggg ccgtcacggg cggaacgggg cgccccgcgc ccgggacggt
     1681 ggtttccctc tggcagtacg actggcagaa gggccatcgg aagatcgccg acgcgaaggc
     1741 ggggccggag ggtctggtct cgttcgaggc gtccccggat tcccggaacg gaacgttcct
     1801 cgtggcgcgg cgcggcgacg acgtcgcgct cgacgcgagc ttcatgtcgc tctggacgcg
     1861 gggcaagtcc ggtgaaatct cctcggctct cgtgtacacg gaccgcagca tctacaggcc
     1921 gctccagaag gtgctctgga aggcggtcgt ctaccgtggc cgcgccgacc aggcgaagtt
     1981 ccgcgtggcc cctgcgacga cggtctcgat cacgctcgtg gacgcgaacg gccaggccgt
     2041 cgagtcgaag accgtgacga cgaacgcgta cggctcggcg gcgggggagt tcgccctccc
     2101 ggccgggcgg gcgctcggcg cgtggaacgt gcgctgtgac tggggcggcg ccgcgtcgat
     2161 ccgcgtcgag gagtacaagc gcccgacgtt cgaggcgacg ctcgccgagg cgaaggaggc
     2221 ccttcgcctg aaccggcccg ccacgttccg cggcgaggcg cgctactact tcggcctgcc
     2281 cgtcgcgaac gggaccgttg cgtggcgcgc gacgcgcgag cccgagtacc cgtggtggtg
     2341 gtggtgggga tggagggggg cgcgcgcggg cggcgtcgag acggtcgcga cgggcaacgc
     2401 ggctctcggc gcggacggga cgttcgcgat cacgttcacg ccgaaggccg acgagcggca
     2461 gtcgaaggag atcacgtacc gctaccgcgt gtcggcggac gtcacggacg agggcggcga
     2521 aacgcgatcg gccgaccggt ccttccgtct cgggttcgtg ggcgtgaaag caagattaga
     2581 gagagaaaga ggattcttct tagaaggtaa tagggcagga gcgacgttcg tgcgctccga
     2641 cctggacggc gcccctcgtg ccggcagggg agcgtggcgg ttgctccgcc tcgtgtcgcc
     2701 cgagaaagcg gtgctccctg ctgaattacc ttctaagaaa tccgaaatcg gcgcgtcgtc
     2761 tcgcgacaac ggggccgcgc tggcagggga catcatgacc cccggcgatc tcctgcgacc
     2821 gcgttggtcg acgggctact ccgtcgacga atggctgcac tcctggaagg acggggctga
     2881 gcaggcctcg ggctcgctcg tccacgacgc gaagggcgag gcgaagctgg agttgccggc
     2941 gctttcctcg ggcgcgtacc gcctgcgcta ctcgaccgtc gacgacttcg gcgcgaagta
     3001 cgaaatggcg caggacttcc tcgtcgccgg cggcaagacg gctcccgtgc tgccggtcgt
     3061 ccttctggcc gagtcctcct ccgtgcccgt cggcggggtt gcgcggttcc tcgtcgcctc
     3121 gggtctccgc gaccagacga tgttcctcga cgtggcgcgc gcgggccggc cgatcgagcg
     3181 gcgcgtcctg aaggcgggca ccgacccggt cgtgatcgag cggaccatca cggaggacga
     3241 ccgcgggggc ctcggcgtca cgctgaccgc cgtgcgcgac caccagttcc tccagcagat
     3301 gcagtccgtg ttcgtgccgt gggacgaccg cgagctgaag gtctccttcg cgacgttccg
     3361 cgaccgcctg cgcccgggcc agaccgagac gtggcgcgtg accgtgaagg gtgcgacgcc
     3421 ggaccacccg ctcagggagg cggccgagct ccttgcgtac atgtacgacc gcagcctcga
     3481 ctccttcacg cctcaccatc cgcagagccc gctcttcctc ctcccgaacc gcgcgacgcc
     3541 gctcgtggcg cgcgcaacgc tcggcgcggc gccgcagctc tggctcacga gcagcggctt
     3601 cccgccgccg cccatgccgc ccatcctcgt cggggaccgg ctgaagttcg agagcggcta
     3661 cgggatcgga ggccccggcg tcaggggccg cgggatgggc cgcgcgtaca acgaggcgat
     3721 gcccgccgct ccggcggcca tggcgttgaa atctgccgcg cgccaggaga tggccgtggg
     3781 cgccgtcgcg gaaagcaaga tggctgatgc gaaggacgga aggtccgatg agaccgattc
     3841 actttccaag aagaaagaga acggggcgat gcgcgaggac gcggccggcg acgcttcggc
     3901 gctccggtcg aacttctccg aaaccgcgtt ctggcagccg cagctcctga cgggaacgga
     3961 cggttcggcg tcgatcgagt tcaccgtgcc ggattccgtg acgtcgtgga gggtgttcgt
     4021 ccacgccgtc acgaaggacc tgcgcggagg ctccgtggac gccgagacga agaccgtcaa
     4081 ggacctcatg gtccggccgt acgtcccgcg cttcctgcgc gagggcgaca gggccgagat
     4141 caaggtcgtc gtgaacaacg cgtccgcgaa accgctgagc ggcaggctcg cgttcgagat
     4201 cacggacccg gcgacgggga agagcctcct cccggcgttc ggactcgcgg gggaggccgc
     4261 gtcccgcgcg ttcacggttc ccgcgggcgg cggcacgaac ctctcgttcc ccgtcacggc
     4321 gccgaagggc ctgacgacgg tggccttcaa ggccgtcgcg acggcgggcg acctgtcgga
     4381 cggcgaggtc cggccgctgc cggtgctgcc ggggcggatg cacctcgtcc agtcgcgctt
     4441 cgtcacgctc aagaacaagg atcgcaagac gcttcgcttc gacgaccttg cgaggaacga
     4501 cgacccgagc ctcgtgaacg agcagatggt cgtcaccgtg gacgcccagc tcttctacac
     4561 ggtcctccag gcgctgccgt acctcgtgaa ctacccgtac gagtgcacgg agcagacgct
     4621 gaaccgcttc ctttcgacgg gcatcgtgtc gagcgtctac cgggactatc cggcggtcgc
     4681 gaagatggcg gcggagatgt cgaagcgcac gacgccgctc gagtcgtggg acgcgacgga
     4741 cccgaaccgg aagatggcgc tcgaggagac gccctggctc gcgacggccc aggggaaccg
     4801 gcccggcgag aacgggtcga tcaacgtgct cgatccgcgc gtcgcgaagg cgaaccagga
     4861 gtcggcgctc gcgcggctga agaaggccca gacctcgatc ggcgcgttcc cgtggttccc
     4921 ggggggcccg ccgtcgccgt acatgacgct ctacatcctc cacggcttcg cgaagggcct
     4981 cgaattcggg gtggacgtcc cgaaggacgt gacgacgcgg gcgtggggct acgtcgcaca
     5041 gcactggcgg agcgactggc gtgcgtgcat ggtgcacgac ggctgctggg agttcgtcac
     5101 gttcctgaac tacgtcctct cgacgtaccc cgacgtgtcg tggagcggcg gcgccttcac
     5161 ggacgccgag cgtaaggaga tgctcgactt ctccttccgg cactggcggc agcacgcgcc
     5221 ctacctgaag gggtatctcg cgctgacgct gaagcggtcg ggccgggccg ccgacgcgaa
     5281 gctcgtgtgg gactccgtga tggactcggc gaagtcgaac gacgagcagg gcacgttctg
     5341 ggcgcccgag gaccgctcgt ggctctggta caacgacacg atcgagacgc aggcgttcgc
     5401 gctgcgcacg ctcctcgagc tggaccccgc gaacgggaag atggacggcc tcgtccagtg
     5461 gctgctcctc aacaagaagc tcagccagtg gaagtcgacg cgcgcgacgg cggaggtcat
     5521 ctactcgctc gtctcctacc tgaagaaaca gggcgcgctc ggcgtcccgg aggacgcgac
     5581 cgtgacggtc ggcgtccaga aggtcgcctt caccttcgac cccgagaagt acacgggcaa
     5641 gaacaaccgc atcgtcattc ccggcgagaa ggtcgatccg aagacgacgt cgacggtcgt
     5701 cgtcgagaag gaagggaagg ggttcgtatt cgcgtccgcg gcctggcact tctcgacgga
     5761 gaggctcccc gccgaggacc gcggggactt cttcgccgtc tcgcggaagt acttccggcg
     5821 cgagaacacc gggaaggagt tcacgctcac gccgctcgcg gagggcgcgg tcatccggcc
     5881 cggggaccag atcgaggtgc agatctcgct ccggaccaag cacgaggccg aatacgtcca
     5941 cctgcgcgac ccgcgggcgg cgggcctcga gcccgagaac gtcctctcga ggtggaagtg
     6001 ggacctcggg atcgcctggt acgaggagac gcgcgactcg gcgacgaact tcttcttcga
     6061 gcgtctgccc gtgggtgagt acacgttcaa gtaccgtctg cgcgcgaaca tggccggcac
     6121 gttccgggtc ggtcccgcga cggtccagtc catgtacgcg cccgagttca acgcgtactc
     6181 ggcgggcgcg gtcctcaggg tggcgggagg atcgcctcca tgaaccgcgt gccctcgatg
     6241 gggtggtcgt tgtacggagg gatgtccagg aatcccagcg aaaggtaaag agcctgggcg
     6301 gccgccatcg aaggcagtgt gtcgaggcgc acggcgcgat agccggccgc gcgcgcctcg
     6361 gccatcaggc tttcggcgag ccgccggccg aggcccgacc cgcgaaaggc gggcctcacc
     6421 cagagcctct tcatctcgca gatctcttct ccgatcttcc tgagagcgat gcagcccgca
     6481 ggctcgtcgc cggcgaacgc gaggaggagg cggccctcgg gcggcgcgta cgcgcccggc
     6541 aagccggcca gctcggcgtc gaagttctgg aagcagagcg agaacccgag cgactgctgg
     6601 tactcctcaa agagggctcg tgccgtcgcg acgtcggctg cgccgtcagc ctttctgatg
     6661 agagtgggca cggtgtacat ccctctttct ctgaaagctc tgagcggcca ggaccaggcc
     6721 gtcgcggtcg atcgcggcga gggcgtcgac ctccgcgggg gagagccgcg ctgcagcagc
     6781 gcggccgcgc gggtccgtga gaaacgcggc ccgttcggcc tcgtcggtgt agattttcgc
     6841 gagatacgcc tcgaatgcgg ggctcacgcg gccgcctccg cccggcgagc cctgcccgcg
     6901 gcgagcgagg ctcgggcacg ggagagctcc gagaggattt cttcgaaaag tggaaaggcg
     6961 ccatcgcgct cgaggatcac ggtgagcggg cgggggaccg ccgccgcgac ggattcgagg
     7021 agggtgaaga cgacgtcggg gaccgcgtgg aggtggtcgt cgaggatccg cgtctcacct
     7081 tcttggcccg gcaccccaat gctccggccc cccgccagat gtatcgccct cacgcgctgc
     7141 gtcgggatcc ttctaagaaa atcttcctct tcgaatccga aattcctggc gttcgcgtgc
     7201 agattgtgga ggtcgaggag gaggtccgtg ccgcaggcct cgaggacgtc cgcgacaaac
     7261 gtcgcctcgt cgcggtcgct gccgggcggg tcgatcagcg tcgcgacgtt ctcgaggagc
     7321 ggcgcgctgc cgacgacggc gcgggcgcgc gcgacgttgc gcgccgttcc ctcgatcgtg
     7381 gccgcacacc gcggcggagc ggcgaggtgg ccgatctcga tcccgccgcc gcgcacgaac
     7441 gcgaggtgct cggaccagga cacgggctcg gcgagctcga cgacgcgcgc gaaggcgtcg
     7501 aggcgcttcg ggtggacggg agcggacgag gcgaggccga gcgagacgcc gtgcaggacg
     7561 accggcacgt gcgccccgag cgtccgcagg gcgcggacct cccgcgccgg gcgcttcatc
     7621 cagtcttcgg cgatcacctc cacgacgtcc agccggtccg ccgccgcgaa gatgcggtcc
     7681 gcgatcgcgg gccgccagcc gagtccgacg aagtccttca cgtcagcctc cgcacccgcc
     7741 gcagccgcct ccgcatccgc cgccgcagga cgatccgcac gaggacccgc aggacgagcc
     7801 gcaggacgac gaagcagtcc cgctcgccga cgccttcgga aagagcttct tcgcccattc
     7861 gaaacctgcc gccggaagga ggccgacgcc gaacacggcg gcgagaaggg cgacctcggc
     7921 cgagcccgcg tgcggccgca gcgaagcggc ccggcccttc aggcccgaga agagcgtccg
     7981 gatgtccgcg aggagccggt cgcccttcgc ggtccgccgc ggaaacgccg cggcgagcgc
     8041 ggcgacggcc gcgaagatcg acaggatgac caggaaggcg acgttgtgcc ggccgcggga
     8101 gaacgcctgc agcaggcgtg cgccggaaac ggcccagagg actccgagac cgaggacgaa
     8161 gacgcgggtg cgggcggcgt tctctgcggg ccccgggagg aggccgaggt ccgccagtga
     8221 gtttcggacg ggcacggtcg ccgcctcgag actcttgtcg ttgaagatcg aggtggcctc
     8281 gccgaaggac cggaatttct cgaggagcac ggcctcgaga gcgttctgcg catgcgcgcc
     8341 cttcttcgcc tggagcgtcg ttcccgacgg gacgagcagg ccgcggtcca cgagcgcgac
     8401 gctcgcgacg cgcagcgtct cgttcggtcc tccccgcagg tacgcgatcg cgtaagggtc
     8461 cgtgagcgag acccgcatcg ggtcgccgct ctcggcgctc ttccgcatgc gcgcgatcac
     8521 ccagaccgtc gcggccgcaa aggcgacgta gaacagcagg aactgcgggc cggtcagcgc
     8581 gaagggattc atgacgcgtg gcgggcgcgc gcgcgcagga gagaggcgag ttcgggaagc
     8641 cggccgagga tgcgcgccgc ctcgcccggg ccgagggggc gctgctcgcg cacggcagag
     8701 aggctctcga cgagggcggg ccagccctcc ccgggcaggt ctgcggcggc ctgccgcagg
     8761 gcttccttcg gcgaggggac ggcgcggcct tccagcgaga ggagcgacgc cgcggccgcg
     8821 cgcagcgggc ccgtgaaatt cgagacggag cgcgccgcct cgtcgtcact cgcgccgtgg
     8881 agcgcccagg cctcgcggag acgcagcatg aggttgagga gcgactgcga aaggcgccgg
     8941 cgcagggcgt cctccggcac cttcagcccc gcgaacggat cggtgccgaa gaggacgcgg
     9001 cggcgccgcg cgatgtccgc gaacttgacc gcgaacgcct cggcggcctc cgcgatctcg
     9061 ctctcgagga gaaacatgac ctcgagacgg gcggccgcct gcgcgaggcg cagggcgccg
     9121 cggaggttct cgacgcttct ctccggaagc gccttcagga cgaggacgac gttgacgtcg
     9181 gactcgggcc ggagcgcccc ttccgccgcg ctgccgaaga gaacggccga ggagaggtcg
     9241 ccgcccaggc tcgtgcgcgc ggcggcgaga aactcgtcga gggctcgggc ggccggctcg
     9301 ggaagttcca tggcgaaatc ctcgcttctc cgtctccgga ctacctggag atgccgatgt
     9361 cggccgcgtt cggcgcaccc gtcgacgcgg tccgtgcgcg cgcccgggcg agcagtcgct
     9421 cccggaagag agcgggtggg atctggccct cggacaggaa ctcctcgtgg aagcgcttct
     9481 ccgagaacgc cgccccctcg attcgcctgt attcctcccg cagggagagg atctcggcct
     9541 tcccgaggtg atacgtgatg gcctgcgtcg gccacttgga gtaccggaac gcggctttct
     9601 tcatcccctc cacggcggcg cgttcggcgg cgctcgggtt gaccttcggg tcggtggaga
     9661 cggggccttg cacgaggcgc acgttgcgcg cgagatacgc cacggcctcg tcgaacgtca
     9721 tgaagccgca gtgcaggccc gtatccacga cgacgcgcgc gtcgcgcagg agctggttct
     9781 ggagctggat gaggcgttcc tccggcgtgt agaagccctc ggggtacccg tcccgcggct
     9841 cgccgatcaa ctgctcggta tagagggccc agccctcgga ggacagcgcg tcctcccaca
     9901 tcgacgccga gtcctcgacg ccgcccggca gcagccagcg gatcacgggg atcgacgccg
     9961 agcgcgcgcg cgtgaactgg tagtaccagt cgtgtccggg aaagccctcg tgggcggccg
    10021 tggacgcgat cgcgggccgg ttgtggtcgc gcagcgcggc gggatcgttc cccgtcggcg
    10081 tcacgtagaa ctgcccgacg ccgccgtgct tgaactgggg ggcggggtag tacgcggcgt
    10141 cgatcgaatc gcgcagcggc ggcggcgtgt agaggacgtc cagccggtag tcggccggca
    10201 ggtcgaacat gcccgtcttc cggccgtacg cgacgaggcg ctggcaggcg tcccggtagg
    10261 acgcgagcat ctccgcgtcg gtcttcggga tgtcgtcctc cagcttcgcg acgacggcgg
    10321 gcagggacgc gtcggcccag aggcgcgaga gggcgatgcg gcgggcgagg gccgtcatct
    10381 cggaaagcgt cgccgcgacg gcggtcttgc cgcgcgcgtg cagctcgcgc ggcgtcgcgg
    10441 agatccccag gttgttcttg agcgcccagg tgtattcgat ctcgccggcc gcgtagcggt
    10501 cgcggtcgaa ggcgggcttc agcgtcttgc cgtcgggctg gtagaagagg tcgacgaggc
    10561 ccttgcggaa gtcgcggaac gcggccgcgg cggctgcgga cgccgagagg agagccgcgc
    10621 ggccggcctc gtcgagggcg gggccggcct ccttcgaacg ctccgggagc gtcttctcga
    10681 agtaggccgc cgtcgccgcc gccgcgtcga ccgacacggc gacgaggcgc cggtccggga
    10741 tcgcgtcgcc ggaggcgccg cgccggacgt tcgcgagagc cgctttcagg tatgcgggca
    10801 ccgcggcggt gcgggaggcg atcgcgtccc attcggcctt cgtcccgcgc gcgccgccgg
    10861 cggccggctc cattccctgg agcgtccact cgatcccgcg gagcggctcc tcgaggaaga
    10921 cgtcgagcgc cttctcgtgg atccggcgtt cgtggttgtg gatcaggaag gcgagatggg
    10981 ccttcatgac ggccgcgtcg atgcggtcgg attccgtcag cttcttccgg tcgaccttcg
    11041 cgaggcgcgc ctcgaaaccg cgccactcgt cgcgttcggc gaggagggcc gccggcgcgt
    11101 agtcgcgaag ctttccgtcc agcgcggcgt acgcagggtc gagcccggag gcgccgaggt
    11161 acgtcgcgac gacggggaag cggttcagga acgcgcggac gtagtcggcc tcgagcgttg
    11221 cgaaggcaga aggagatttc tgcttcgggg aagagacggg ggatttcgga ggggaggatt
    11281 tctgagaatg cgcggggagg gccgccgcaa gggccagcat gaatgccgcg ctggctgccg
    11341 gccgtgcgaa tcgcctgttc acccttcgaa gcactttgca gaccctcccc cggcgcggcg
    11401 acgctagtcg cgagggggat tctctctcaa ccgcccgagg aagaacgcca gccccaccgc
    11461 tcccgccgcg agcgtcgtca ggaggtgcag gaggccgccg aggaccgcgc ccgtggcgag
    11521 cgacagcccg tccagcgccg cggccgccgc gaagccggat tcgtacgggc cgaacgtgcc
    11581 cgccggcgac gggatcaccg ctgcggtcgt gatggacgcg aggagcgcga cggcgagcgt
    11641 ggccgggtgc agcggaaggc cgaggcccct cgccgtcacg aagagcgcgg cgaggtggag
    11701 cgcgaggaag gcgaacgccg cgagcgccgc gccgaggtag cgtcccggcg cgcggcgcgc
    11761 cacggcgagg ccctcgagga tctcctggga cacgtcttct ccttgcggtt ctggtctcac
    11821 ggacaggggt cgtgcggatc gtgcacccgg tgccggtacg gcgcgggaat caggggagct
    11881 cgacgacctg ggcggcgtag gcgaggcctg c
//
LOCUS       DAOMFY010000106        11736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1697_length_11736_cov_8.397141, whole genome shotgun sequence.
ACCESSION   DAOMFY010000106 DAOMFY010000000
VERSION     DAOMFY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1697_length_11736_cov_8.397141"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLB01_10215"
     CDS             <1..764
                     /locus_tag="PLB01_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77718.1"
                     /translation="QEAWLASEDPAVRRKAWRVAALCALPQARTFWERGLSDPDPGVR
                     SAAREAALWCRQSWALDDVRRGRRRSDGADPDSLYLVAATGDAADLQLVLAWVKDVGA
                     GPERYRVIGVLGHPGGVETLLHAMAGLDLAASAFAGQAFTKLTGVDVDSGRRTVVPPA
                     DGTEPDSFEAEFLDEMPLPDERQARKAWETRRSEFAKGTRWCRGVDLSRGLPEAALEA
                     LDRESLWEARLRGILAGTWKGRLADLEAFPQKRRA"
     gene            774..1325
                     /locus_tag="PLB01_10220"
     CDS             774..1325
                     /locus_tag="PLB01_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77719.1"
                     /translation="MADVYGLTRGMADAIVKAVRDDAQCRLLLPRVSAFKVFGGIGHA
                     GTARNFANAWNMSLADADKFVMDKAGFPEAGNLTRDYWRRLGDRQACTGVAETTARVL
                     RAAVGKAGYEFLVSAGPQDRTPSDPPHDYHTATAVTVTGDHVYVFDWHSTLDVGDPLI
                     FPSPAAFKAGRPSMRYSLFWGWT"
     gene            1418..2593
                     /locus_tag="PLB01_10225"
     CDS             1418..2593
                     /locus_tag="PLB01_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77720.1"
                     /translation="MAPPPNAPHYAQRAYIDHWSPTGRAEPPSMAGRMPPPSGLGLPA
                     VFEYDVHGDLITDASDMSFPPRHGDPCYRVGDCVLVQSIRRSLLGATQNWRDLGRRPE
                     LGYWIILALLVNDSDEVTVGYKIRSITPTGGGDGTVQLARVAVVEGRPGRGGAQILQD
                     APGGPAVWTRPNIDENLTEFNSAGLVPIYRWVDEHAPDFFLAGMGTAVSATEEITQRG
                     IRAALRKAAKAGRTALLKSFMAALKEYAKCVWPLVRAEAQLKQRPVDAAMVFEVAEKN
                     PAGRREALDKFVETFIDETLGEWIGSSVDRATKLQMEQTLLNSTLFRPFANLVTTRMI
                     QAAREAAFQTWVVGASRPDDFPDSFMSAFCRQAADEARGVFIPPGVSASAVHDQAGS"
     gene            complement(2832..3893)
                     /locus_tag="PLB01_10230"
     CDS             complement(2832..3893)
                     /locus_tag="PLB01_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77721.1"
                     /translation="MANFFDAFSGKDITTLRVTKGSKFRIGLYGGAPGGVKLNVTTAK
                     DGSDAKAPDPAVKFIAEQGSWRLIYEIDSNRIQSEKVRACWDGNDYSAPVALTLGAGA
                     GGGKADVRRAIVQLARTFVPNAHYLWGTAGNTPGSGDGNPGGGKMAAAVMRSPSLNPS
                     ETAKDKVLGVCMAVQPQFDGYNTCAGRSRGRTGSLDEFLKACQAKIDAGQTDQTTWPG
                     GGGGNLYPRKYHFRGDLAGNGNVVWGESCAGVRHFDCVGLVNYCYGKHWYRGAFGLDI
                     KFFRNPMAGTVSLTGESDLMDADILIKPGDVHIGMLFRNGQSWSVVQAESTEIGLTDT
                     AVFDPKEWDRYRMQDAFLV"
     gene            complement(3936..5429)
                     /locus_tag="PLB01_10235"
     CDS             complement(3936..5429)
                     /locus_tag="PLB01_10235"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HQQ77722.1"
                     /translation="MSKRPPSHDVDVRLGSGTQASPEPLDPETPFRILVIGDFGGAGA
                     GPLAARALHAVDRDVLEEELARLQAVVDAPLGGGRGAKITLRDLDDFHPDRLLERVDT
                     LAGLRRTRERLEDPRTFQEAAGEIRAWAGPPPEPRERAAAPAGAGDDIVGELLAGASR
                     RAKAADTAFEAELKAIVRPHLAPEEDADLPRLRDAVDEARSGLLRAILRSPRFREVEA
                     AWRSLDLLVRRIGDEAVRVFFLDVSRASLVEDLSSHEKLSGTGLFRRLVTETLDTPGA
                     EPWGAVVGLYDFGPTLADAALLSRIAKIAQAAGAPFLAGARPELVGCASLARTPDPDD
                     WKKPEDDGAVIWEELRALPEAGWVALALPRLMLRLPYGAKTDPIEAFPFEEIPHAPEH
                     EAYLWGNAALACALVLAGAFELGGWNLRPETAAEVQGLPAHVFKDGLEMRVTPPSEVV
                     LTLRAAERILEAGLVPVLSLKGSESVRVARLQPIASPLRALAGRWLR"
     gene            5505..7046
                     /locus_tag="PLB01_10240"
     CDS             5505..7046
                     /locus_tag="PLB01_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ77723.1"
                     /translation="MPEKEPTAYDAVSYPGSPFSQTHPDRLATIAALYGLKAAPPGSC
                     RVLELGCGNGANLIPMAYGLPGGTFVGLDAARSAVEHGRAQIAALGLTNITLLHADLL
                     HAADLGTFDYVVAHGLYSWVPPPVQERVLEIAAEAVAPNGVVYVSYNALPGCHVRNAM
                     RQMMRWHVRGMEEPAERIGQARGLVRFLAEAAPKRPVFSAILAETLEHQEKQPDAVLF
                     HDDLAEVNEPLLFVDFLERAGRHRLKFLSEADYCDMAVWDADSPAGRFLLDVGSENLV
                     LQQQYRDFLTFRRFRQTLLCREETALRQEPDPEVLRALYVGANTRPKGPHPDVASDEP
                     LSFVAEKDRELATPHPLSKAAFLELGEISPRWISFPELLAAARARLSAAGGRPATAED
                     EARLLRLLLASYGANVSELRSTACPFVTRVSEHPRASALARLQARTLPDVTNLMHGSV
                     RLEDPHVRTFLGLLDGTRDRTAIERAMAAALREDPATAALEADVEKGIALNLDRVSRL
                     ALLES"
     gene            complement(7060..7746)
                     /locus_tag="PLB01_10245"
     CDS             complement(7060..7746)
                     /locus_tag="PLB01_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77724.1"
                     /translation="MTVGLNSLRYRNAAREGFTKTSPEMLAKDAVVGKAGAAWTTRIQ
                     SAFSGKGSPESIAKALELVAEHELWEKGGIPEMAALKTLPLPIEEKLRRYCDRMFGLD
                     CLGFVINYVQSQMNLLLDKSPLFADLHHFRTAPFTPRMTLLSFRMGDIVVWDEPPRHI
                     ALIGSLQNQTFAFRVPLVMSAADGNFGPAGLVLDDFTLKADGVPGSFTFVGKFDSQRV
                     RVFGPPIPIF"
     gene            complement(7840..8616)
                     /locus_tag="PLB01_10250"
     CDS             complement(7840..8616)
                     /locus_tag="PLB01_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77725.1"
                     /translation="MDEGFDLQKLRSLRKLTRAIAESLRSELREHLTTMTLLLRPRTI
                     LGDFVEGSVKGEVKGADAAFRDLRSRYESVAGGRPFGLPKELNPPIEILSSNVELVPL
                     EYPHVAKSGSESKTVTVTQPLTWILTYSGLAPTRLRELMAAKIPDSAQVKDVILHALV
                     LDTVLTKQPGLLAVFEALRFPVVTQKLPGLGELPVTCISSSVRTVRPPDDVLIESTEI
                     SGLNAFEEVVNAEDIVEMQDPFREKLLEVIKANAPEALKG"
     gene            complement(8650..9624)
                     /locus_tag="PLB01_10255"
     CDS             complement(8650..9624)
                     /locus_tag="PLB01_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77726.1"
                     /translation="MPQAASVPLAAEEPPVTLARLRHYRKLTKAVSAHLRGQLETILG
                     TLTPLFHAETVFGDYVQRSVLSAKPEADAGPRAQLAPDRWGRGSELAVKRDAAAPPTV
                     KELEDARKYAKANISIKEAIKGADRAFQDLESLYESVAKRRPYDLGLGLTPPLPINTS
                     ALEILPLDYPYVARSERQTKRVMVAAPLTWIVTYAGFPLSRLRALIADPNRSRDELQR
                     VLTHHLLLHVVLERKSGLRGLLHALNFSVRTEKLPEFGELPLTVITSAVKTERPADEV
                     ILESTDLSGVDATEEIVRVTNVAGMVDARRARLLEIMEAEKSGDNGDA"
     gene            complement(9829..10566)
                     /locus_tag="PLB01_10260"
     CDS             complement(9829..10566)
                     /locus_tag="PLB01_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQQ77727.1"
                     /translation="MTVRVGMKVWDDDVEMEMTLPAGAARLGALLPVFQRVADGLVGM
                     AVDRAGAEGRSVSCRKGCGACCRQLVPISEVEARHIGRLVEELPEPRRAEVRARFVKA
                     LERLAEAGLLAKLENRGNLEEADARSMGLAYFNLGIPCPFLEDESCSIHADRPITCRE
                     YLVTSPAVNCADPKAESIDQVEMPGKTWTALARAGSTGPPARFIPWVPLVLAPDWASR
                     HPDESSPRPVEDLFRGVFAELAGRKGA"
     gene            10683..11495
                     /locus_tag="PLB01_10265"
     CDS             10683..11495
                     /locus_tag="PLB01_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77728.1"
                     /translation="MPSWIPGYDLIEGPEETGIPFRYRAVREADGAAAWIRVDGFDSS
                     SSRAAQTVDALYTATSRFDHPLLPNVLDFGSAAVTGRTYIAFQTSEPVAGGGHRTRRG
                     TLTALAELQIVALECGFEGVHLPLHELLRLREGVRLEARHLPLNEFLCEPHRDDGRIA
                     LAGLEAEDEGAPFLAPESRSGSPGRSRRAALAYRLAAQIHVLLEGHPPGRSPVEDADK
                     TVALSALPFPPRSSVETDVPRELVRLGLSPRPEDRPDLEHFLTVRLPSPKTA"
     gene            11537..>11736
                     /locus_tag="PLB01_10270"
     CDS             11537..>11736
                     /locus_tag="PLB01_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HQQ77729.1"
                     /translation="MRDGRNSRKQVLFATAVAAFLMASAAPAQTPIPTLPPLPPLPAA
                     AGAATPAPTVAPPATEPLSTPAP"
BASE COUNT         1784 a         4137 c         3983 g         1832 t
ORIGIN      
        1 cgcaggaagc ctggctcgcc tccgaggatc ccgccgtccg ccgcaaggcc tggcgcgtcg
       61 ccgccctctg cgcactcccg caggcccgga ccttctggga gcgcggcctc tcggacccgg
      121 accccggtgt gcgctcggcg gcacgggaag cggccctctg gtgccggcag tcctgggcgc
      181 tcgacgacgt ccgccgcggc cggaggcgct cggacggcgc agacccggac tccctgtacc
      241 tagtcgccgc gacgggcgac gcggcggatc tccagctcgt tctcgcgtgg gtgaaggatg
      301 tcggtgccgg ccccgagcgc taccgtgtga tcggcgtcct cggccacccc gggggggtgg
      361 agacgctcct ccacgccatg gcgggactcg acctcgcggc gtccgcgttc gccgggcagg
      421 ctttcacgaa gctgaccggt gtggacgtcg actccggccg gcgcacggtc gttccacccg
      481 ccgacggcac ggaaccggat tcgttcgaag cggaattcct cgacgagatg ccgctgcccg
      541 acgagcggca ggcccggaag gcctgggaaa cgcgccgatc ggaattcgcg aagggaacac
      601 gatggtgccg cggagtcgat ctgtcgcggg ggctcccgga ggctgcgctc gaggctctcg
      661 accgggaatc cctctgggag gcacgcctcc gtggcattct cgccgggacc tggaagggcc
      721 gcctcgcaga cctcgaggcc ttcccgcaga agcgccgggc gtaggggggg acgatggcgg
      781 acgtatacgg tctgacccgt ggcatggccg acgcgatcgt gaaagcggtg cgggacgacg
      841 cacaatgccg cctcctgctg ccgcgcgtga gcgccttcaa ggtcttcgga ggaatcggcc
      901 acgcgggcac ggcgcggaac ttcgccaacg cctggaacat gagcctggcg gatgcggaca
      961 agttcgtgat ggacaaggcc ggattcccgg aagccggaaa cctcacgcgg gactactggc
     1021 ggcgtctcgg tgaccgccag gcctgcacgg gtgtggccga gaccacggcg cgtgtcctga
     1081 gggcggccgt ggggaaggcc ggctacgagt ttctggtcag cgccggcccg caggaccgca
     1141 cgcccagcga cccgccgcac gactatcaca cggccaccgc ggtcaccgtg accggcgacc
     1201 acgtgtacgt gttcgactgg cattccacgc tcgacgtcgg cgatccgctc atctttccct
     1261 cgccggcggc cttcaaggcc ggccgcccct cgatgcggta ctcgctgttc tggggctgga
     1321 cctagaagcc gctccggaaa gcccttgttc ggaacccgcc cccgtcacat gatccgcctg
     1381 acttccgccc ggagtgcatg ttcagggagg tgacgccatg gcccctcctc caaacgctcc
     1441 tcattacgca cagcgggcgt acatcgacca ctggtcaccg acgggacgcg ccgagccgcc
     1501 ctcgatggcc ggccggatgc ccccgccgtc cggactcggt ctgcccgcgg ttttcgagta
     1561 cgacgttcac ggggacctga tcacggacgc gagcgacatg tcctttccgc cgcgtcacgg
     1621 ggacccgtgc taccgagtgg gcgactgcgt gctggtccag tcgattcgcc gcagcctgct
     1681 cggggccacg cagaactggc gcgacctcgg tcggaggcct gaactcggct actggatcat
     1741 cctggcgctc ctcgtgaacg actcggacga ggtgaccgtc ggctacaaga tacgaagcat
     1801 cacgccgacc ggcggcggcg atggcaccgt gcagcttgcg cgggtggccg tcgtggaagg
     1861 ccggcccggt cgcggtggag cgcagatcct ccaggacgcc ccgggaggac cggccgtctg
     1921 gactcgcccg aacatcgacg agaacctgac ggagttcaac tcggcgggcc tcgtgccgat
     1981 ctaccggtgg gtcgacgagc acgcgccgga tttctttctc gcgggcatgg ggacggctgt
     2041 ctccgcgacc gaggagatca cgcagcgagg catccgtgct gctctgagga aggcggccaa
     2101 ggcgggacgg accgctctcc tgaagtcgtt catggcggca ctcaaggagt acgcgaagtg
     2161 cgtctggccc ctcgtgcggg ccgaggctca gctgaaacag cgccccgtgg acgccgcaat
     2221 ggtgttcgag gtggccgaga agaaccccgc cggccgcagg gaggcgctcg acaagttcgt
     2281 cgagacgttc atcgacgaga cgttggggga gtggatcggg tcgagcgtcg accgagcgac
     2341 aaagctgcag atggaacaaa cgctcttgaa cagcacgctg ttccgtccgt ttgcgaacct
     2401 ggtgacgacc cgcatgattc aggcggcccg ggaggcggcg ttccagacat gggtcgtggg
     2461 ggcgagtcgt ccggacgact ttccggactc gttcatgagc gccttctgcc gtcaggccgc
     2521 cgacgaggcg cgcggcgtct tcatcccgcc gggtgtctcg gcttcggccg tccacgacca
     2581 ggccggttca tgaccgaagg gcgcccgcct accgcctcgc gaggatgaag tcggggacga
     2641 ccatcgcgcc ttcgaaggtc tctgatccgg cacgatgtcg tgcgcccgcg tgaggttctt
     2701 cacgtgatcg accccggccg acgaatgccg ccccatcacc atggcgatcg ccgtggccca
     2761 gcaatcatgg gggttcgcct gctgaaggtg tgcgacgtgg tggcggatct cgatcgacat
     2821 ggtgtcgaac gctacaccag gaacgcgtcc tgcatgcggt agcgatccca ttccttgggg
     2881 tcgaaaaccg cggtatcggt caggccgatc tcggtgctct cggcctggac gacggaccac
     2941 gactggccgt tccggaagag catgccgatg tgcacgtcgc ccggcttgat caggatgtcg
     3001 gcgtccatca ggtcgctttc tcccgtgaga gacaccgtgc ccgccatcgg gttccggaag
     3061 aacttgatgt cgagaccgaa cgcccccctg taccagtgct tgccgtagca gtagttcacc
     3121 agcccgacgc agtcgaagtg ccggacgccc gcgcaggact cgccccagac cacgttgccg
     3181 tttcccgcga ggtcgccccg aaagtggtac ttgcggggat acaggttgcc gcctccgccg
     3241 ccgggccacg tcgtctggtc cgtctggccg gcgtcgatct tggcctgaca ggccttgagg
     3301 aactcgtcga ggctgccggt cctgccccgc gagcgaccgg cgcaggtgtt gtaaccgtcg
     3361 aactgcggct gcacggccat gcacaccccg aggaccttat cttttgccgt ttcggacggg
     3421 ttcaggctgg gggaacgcat cacggccgcc gccattttgc cgccgcccgg attcccgtct
     3481 ccgctgcccg gcgtgttgcc ggccgtgccc cacaggtaat gcgcgttcgg aacgaaggtg
     3541 cgggcgagct ggacgatggc ccggcgaaca tcggcctttc cgccgccggc gccggcgccg
     3601 agcgtcagcg cgacgggagc cgagtagtcg ttgccgtccc agcaggcgcg cactttctcg
     3661 ctctggatcc ggttcgagtc gatctcgtag atcaggcgcc acgagccctg ctcggcaatg
     3721 aacttgacgg cggggtccgg cgccttcgcg tcgcttccgt ccttggccgt cgtcacgttc
     3781 agtttgaccc cgccgggtgc gccgccgtag aggccgatcc ggaacttcga gcccttggtg
     3841 acgcgcaggg tcgtgatgtc cttgccggaa aacgcgtcga agaagttcgc catgacgctc
     3901 cctttcccac gaatcctgag cggcagccgg caccgtcagc gcagccatct cccggcgagc
     3961 gcgcgcagcg ggctcgcgat tggctgaagt cgcgcgacgc gcacgctctc gctccccttc
     4021 aacgacagaa ccggaacgag cccggcctcg agaatcctct ccgcggcgcg gagagtcagg
     4081 acgacctcgg acggcggagt gacccgcatt tcgaggccgt ccttgaagac gtgcgcgggc
     4141 aggccctgga cctcggccgc cgtctcgggc ctgaggttcc atccgcccag ctcgaacgcg
     4201 ccggcgagca cgagcgcgca ggcgagggcc gcgtttcccc agaggtaggc ctcgtgttcc
     4261 ggggcgtgcg ggatctcctc gaaggggaaa gcctcgatcg gatcggtctt cgcaccgtac
     4321 gggaggcgca acatgagacg aggcagcgcg agcgcaaccc agccggcttc cggcagagcg
     4381 cgcagctcct cccagatgac ggccccgtca tcctcgggct tcttccagtc gtcgggatcc
     4441 ggcgtccggg cgagggacgc acagccgacg agctccggac gggcgcccgc gaggaaggga
     4501 gccccggccg cctgggcgat cttggcgatg cgggacagca gcgccgcgtc ggcgagcgtc
     4561 gggccgaagt cgtagagccc gacgacggct ccccacggct ccgcacccgg cgtgtcgagc
     4621 gtctccgtca cgaggcgccg gaagagtccc gttccggaaa gcttctcgtg gctcgaaagg
     4681 tcctccacga gcgatgcgcg cgacacgtcg aggaagaaga cgcgaacggc ctcgtccccg
     4741 atccggcgca cgagcaggtc gagagaacgc cacgcggcct cgacctcccg gaagcggggg
     4801 ctgcggagga tcgcgcggag cagtcccgag cgcgcctcgt ccaccgcatc cctcaggcgc
     4861 ggcaggtcgg cgtcctcttc cggggcgaga tggggccgca cgatcgcctt cagttcggcc
     4921 tcgaacgcgg tatccgcggc cttcgcccgg cgcgaggccc ccgcgaggag ctcgccgacg
     4981 atgtcgtccc ctgcgccggc cggcgcggcg gctctttctc gtggctcggg cggtgggccg
     5041 gcccacgcgc gaatctcccc ggccgcctcc tggaacgtcc ggggatcctc gagccgctcg
     5101 cgcgtcctcc gcaacccggc cagggtgtcg acccgctcga ggagtcggtc cgggtggaag
     5161 tcgtcgaggt cgcgcagcgt gatcttcgcg ccgcgcccgc cgccgagcgg cgcgtccacg
     5221 accgcctgaa gacgtgcgag ctcctcctcg agtacgtccc ggtcgacggc gtgcagcgct
     5281 cgcgccgcga gcgggccggc gcccgcgccg ccgaagtccc cgatcacgag gatgcgaaac
     5341 ggcgtttcgg ggtcgagcgg ttccggcgac gcctgggttc cgctccccag cctcacgtcg
     5401 acgtcgtggg acggcgggcg cttggacatc gcggcctcct cgaactgcgg cccgcagtct
     5461 aacgcggctg cgggcgagtc tccgttctat gatcgcgtcc ccccatgccg gaaaaagagc
     5521 cgaccgccta cgacgcggtc tcgtatcccg ggtcgccgtt ctcccagacc catccggacc
     5581 ggctcgcgac gatcgcggcc ctgtacggcc tgaaggccgc tcccccgggg agctgccgcg
     5641 tgctcgagct gggctgcggg aacggggcca acctcatccc gatggcctat ggccttccgg
     5701 gaggcacgtt cgtcggcctg gacgccgcgc gctcggccgt cgagcacggc cgcgcgcaga
     5761 tcgccgccct cggcctgacg aacatcacgc tccttcacgc ggaccttctg cacgcggcgg
     5821 acctggggac gttcgactac gtcgtcgcgc acggcctgta ctcgtgggtt ccgccgcccg
     5881 tgcaggagag ggtcctcgag atcgccgccg aggcggtcgc tccgaacggc gtcgtgtacg
     5941 tgagctacaa cgcgctcccc ggatgccacg tccggaacgc catgcgacag atgatgcgct
     6001 ggcacgtgcg cggcatggag gaacccgccg agaggatcgg ccaggcgcgg ggcctcgtcc
     6061 ggttccttgc ggaggccgca ccgaagcggc ccgtcttctc ggcgatcctg gccgagacgc
     6121 tcgaacacca ggaaaaacag ccggatgccg tcctgttcca cgacgacctc gccgaggtca
     6181 acgagccgct cctcttcgtc gactttctcg agcgcgcggg ccgtcaccgc ctcaagttcc
     6241 tctcggaggc cgactactgc gacatggccg tctgggacgc cgacagtcct gcgggccggt
     6301 tcctcctcga cgtgggatcc gagaacctcg tcctgcagca gcagtaccgg gacttcctca
     6361 cgtttcggcg cttccggcag actcttcttt gccgcgagga gaccgccctc cggcaagagc
     6421 ccgatcccga ggtccttcga gcgctgtacg tcggcgcgaa cacgcggccg aagggccctc
     6481 atcccgacgt cgcctccgac gagcccctga gcttcgtggc cgagaaggac cgggagctcg
     6541 cgacgccgca cccgctctcg aaagccgcgt ttctcgagct cggagagatc tcgccgcgct
     6601 ggatctcgtt ccccgaactt ctcgccgccg cccgcgcgcg actctccgcg gccggagggc
     6661 gacccgccac ggcggaggac gaggcacggc tcctccgcct gctgctcgcc tcctacgggg
     6721 ccaacgtgag cgagctccgg agcacggcct gcccgttcgt cacgcgggtc tcggagcacc
     6781 cccgtgcgag cgcactggca cggctccagg cccgcaccct ccccgacgtg acgaacctca
     6841 tgcacggaag cgttcggctc gaagaccccc acgtgaggac cttcctcggg cttctcgacg
     6901 ggacgcgaga ccggacggcg atcgaacgcg cgatggcggc cgccctccgg gaagatcccg
     6961 ccacggcggc actcgaggcg gacgtggaaa agggaatcgc cctgaacctc gaccgcgtgt
     7021 cccggctcgc gctgctcgaa agctgaggcg cgggtcttct cagaagatcg ggatcggcgg
     7081 gccgaagacg cgcaccctct gagagtcgaa cttgccgacg aacgtgaacg acccgggaac
     7141 cccgtcggcc ttcaacgtga agtcgtcgag gacgagcccc gcgggcccga agttcccgtc
     7201 ggccgccgac atgacgagcg gaacgcggaa cgcgaacgtc tgattctgca acgagccgat
     7261 cagcgcaatg tgtcgaggcg gctcgtccca gacgacgatg tcgcccatcc ggaagctcag
     7321 caacgtcatg cggggcgtga agggggcggt tcgaaagtga tgcaggtctg cgaagagcgg
     7381 gctcttgtcg aggagaagat tcatctggct ctggacgtag ttgatgacga accccaggca
     7441 gtcgagtccg aacatccggt cgcagtagcg acgcaacttc tcctcgatcg gcagcggcag
     7501 ggtcttgagc gccgccatct cgggaatgcc gcctttttcc cacagctcgt gctccgccac
     7561 gagctccagc gccttcgcga tcgactcggg gcttcccttg ccgctgaacg ccgactggat
     7621 ccgtgtcgtc caggccgccc ccgccttgcc gacgacagcg tccttggcga gcatctcggg
     7681 tgacgtcttg gtgaagccct cccgcgcggc gttgcggtag cggagggagt tcagcccgac
     7741 cgtcacccct ctcggcggtg agccgacgtt gacctgatag gtccgatacc gtttcgcgta
     7801 ctccgcgggt gtctggccca gaaatcctcc gggcgaccct cagcctttca gcgcctccgg
     7861 ggcgttcgcc ttgatgacct cgaggagctt ttcacggaag ggatcctgca tctcgacgat
     7921 gtcctcggcg ttcacgacct cctcgaacgc attcaggccg gagatctccg tgctctcgat
     7981 caggacgtcg tccggagggc ggacggtccg cacgctcgag gagatgcacg tgaccggaag
     8041 ctcgccgaga cccgggagct tctgggtgac gaccgggaat cggagcgcct cgaagaccgc
     8101 gagaagccct ggctgcttcg tcagcaccgt atccagaacg agcgcgtgaa ggatcacgtc
     8161 cttcacctgc gcggagtcgg ggatcttcgc ggccatcagt tccctgagac gcgtgggggc
     8221 gagccccgag tacgtgagga tccacgtgag cggctgcgtc acggtgaccg tcttgctttc
     8281 cgatcccgac ttcgcgacgt gcgggtactc gagcggaacg agctcgacgt tggagctgag
     8341 gatctcgatc gggggattga gctccttcgg aaggccgaac ggccggcctc cggcgaccga
     8401 ctcgtaacgg ctgcgaaggt cgcggaacgc cgcgtcggcg cccttgacct cgcccttgac
     8461 gctgccctcg acgaaatcgc ccaggatcgt gcgcgggcgc agcaggagcg tcatcgtggt
     8521 caggtgttcc ctgagctccg agcgcagcga ttccgcgatc gcgcgcgtca gcttccggag
     8581 cgaccggagt ttctggagat cgaacccttc gtccatctga ggtgaatcct ccgttcggcg
     8641 gcgggacgct caggcgtctc cgttatcgcc ggacttctcg gcttccatga tttcgagcaa
     8701 ccgcgcgcgg cgcgcgtcga ccatgccggc cacgttcgtg acgcgcacga tctcctccgt
     8761 cgcgtcgacg cccgagaggt cggtactttc caggatgacc tcgtcggccg gccgctcggt
     8821 cttcaccgcc gacgtgatca ccgtcagggg gagctcgccg aactccggca gtttctcggt
     8881 cctcacggag aagttcaacg cgtgaaggag tccgcgcagc ccgctcttgc gctccaggac
     8941 gacgtgcagc agcaggtggt gcgtcagcac gcgctggagc tcgtcgcgcg agcggtttgg
     9001 gtccgcaatg agggcgcgca gacgcgagag aggaaacccc gcgtacgtca cgatccacgt
     9061 cagcggggcc gcgaccatga cgcgcttcgt ctgccgctcc gagcgcgcga cgtagggata
     9121 gtcgagcggg aggatctcga gagccgaggt gttgatcggg agaggcggcg tcaggccgag
     9181 cccgagatcg taaggcctcc tcttcgcgac ggactcgtac aggctctcga ggtcctggaa
     9241 cgcgcgatcg gcgcccttga tggcttcctt gatggagatg ttcgccttcg cgtactttcg
     9301 cgcgtcctcc agctccttca cggtcggagg ggcggccgcg tcacgtttga cggccagctc
     9361 ggacccgcgg ccccaccggt ccggagcgag ctgtgcgcgc gggcccgcgt cggcctcggg
     9421 cttcgcggac aggacgcttc gctgcacgta gtccccgaag acggtctcgg catggaacag
     9481 cggcgtcagg gttccgagaa tcgtctcgag ctgcccacgg aggtgcgccg acacggcctt
     9541 cgtcagcttc cgatagtgac gcaggcgggc gagcgtgacc ggcggctcct cggcggccag
     9601 cgggacggag gcggcctgcg gcacggcagc cttcggggcg gcggccttcg gcgctgccgg
     9661 agcctttcga tcctccctgg gggcggaccc ggtttcacgt ttccgagcca ggtgcctact
     9721 ccttccagac ctccggcccg ccaagggcgg cgcgacggag cgctcaaacc gtctgagtct
     9781 gtcgaattgt agtccaaggt gggtccggcc ggcgggttcg acgcccgttc acgcgccttt
     9841 gcggcccgcc agctccgcga agacgccccg gaacagatcc tccacggggc gcggcgacga
     9901 ctcgtcggga tgcctggacg cccaatccgg cgcaaggacg agagggaccc acgggatgaa
     9961 gcgcgcgggg gggccggtcg atcccgcacg cgccagcgcc gtccaggtct ttcccggcat
    10021 ttcgacctga tcgatcgact ccgccttcgg atcggcgcag ttcacggccg gcgaggtcac
    10081 gaggtactca cggcaggtga tcggccggtc cgcgtggatg gagcagctct cgtcctcgag
    10141 aaacgggcag gggatgccga ggttgaagta cgcgaggccc atcgaccggg cgtcggcctc
    10201 ctcgagattc ccccggttct ccagtttcgc gagaaggcct gcctcggcga ggcgctcgag
    10261 ggctttcacg aatcgggcgc ggacctccgc gcgccgcggc tcgggaagct cctcgacgag
    10321 ccgtccgatg tgccgtgctt cgacctctga aatcgggacg agttggcggc agcacgcgcc
    10381 gcatcccttc cggcacgaga cggaccgccc ctcggcgccc gcgcgatcga cggccatgcc
    10441 gacgagaccg tccgcgacac gctggaacac cggcaggagc gcccccagcc gcgcagcccc
    10501 ggcggggagg gtcatctcca tctcgacgtc gtcgtcccag accttcattc cgacgcgaac
    10561 ggtcacgtgg tccatcgagt ttgccggccc ttccctgtgg acgtgtagcg ccccccgcga
    10621 cgcgacgcaa cagaaatcgc gcgggccgct cagcccgcca ttcggcctaa gattccggaa
    10681 cgatgccgtc gtggattccg gggtacgacc tgatcgaggg acccgaagag acggggatcc
    10741 ccttccgtta ccgcgcggtt cgcgaggcgg acggcgccgc ggcgtggatt cgcgtcgacg
    10801 gcttcgactc gtcgtcgagc cgcgccgcgc agacggtgga cgccctctac acggccacct
    10861 cccgtttcga ccatccgctg ctgccgaacg tgctcgattt cggctccgcg gccgtgaccg
    10921 ggcggacgta catcgccttt cagacttccg aaccggtcgc gggcggcggg catcggacgc
    10981 gacggggtac gctgacggct ctcgccgagc tgcagatcgt cgcgctggaa tgcggcttcg
    11041 agggcgtcca cctgcccctg cacgagctcc tgcgcctgcg ggaaggggtg cgcctcgaag
    11101 cgaggcacct gcctctgaac gagttcctct gcgagcccca tcgcgacgac ggaaggatcg
    11161 cgctcgccgg gctcgaagcc gaggacgagg gagcgccctt ccttgcgccg gagagccgct
    11221 ccggttcgcc ggggaggtcg cggcgcgcgg cgctcgccta ccgactggcc gcccagatcc
    11281 acgtccttct cgagggacac ccgccgggcc gttcgccggt cgaagacgcc gacaagaccg
    11341 tcgccctgtc ggccctcccg ttcccgcccc ggtcttccgt cgagaccgac gtgccgaggg
    11401 agctcgtgcg gctcggcctg tccccacgcc ccgaagaccg cccggacctc gagcacttcc
    11461 tgacggtccg gctaccgtcc ccgaaaacgg cgtgagacga tcccgacgca cgcacaaacg
    11521 gatcgaggga agaacgatgc gagatggccg caactcccgg aagcaggtcc tgttcgcgac
    11581 cgccgtcgcc gccttcttga tggcgtcggc agcccccgcg cagacgccga tccccacgct
    11641 gccccccttg ccgccgctcc ccgcggcggc cggagccgcg acgcccgcgc caaccgtcgc
    11701 gccgcccgcg accgagcccc tgtccacacc ggcgcc
//
LOCUS       DAOMFY010000107        11667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1713_length_11667_cov_8.190665, whole genome shotgun sequence.
ACCESSION   DAOMFY010000107 DAOMFY010000000
VERSION     DAOMFY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1713_length_11667_cov_8.190665"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLB01_10275"
     CDS             complement(<1..464)
                     /locus_tag="PLB01_10275"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77730.1"
                     /translation="MPVNLGVGDLLAEAGDLTVRRLSRASTWQKTHGGTIERALLSTG
                     AVSEETLTSALAQAYGLPGVSRATLLSADPDIVECLPAKERRRFRAFPFSLSGKILNI
                     AISDPQNIVLQKNLSIATGFSVEIFVAPDPVLEDVIEKFEGAPAGHAPSPAA"
     gene            complement(585..2336)
                     /locus_tag="PLB01_10280"
     CDS             complement(585..2336)
                     /locus_tag="PLB01_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HQQ77731.1"
                     /translation="MRDLRAFLDALRRENDLAVVEAEADPRLEIPEIHRRVIEEGGPA
                     LLFARPKGSDFPVVTNLFGTPKRLALAFGGRPLEFVRTAARAATELVPPTPASLWKFR
                     SFFGEALRVGTTRARNAPVLDVVETAPDLTRLPALTQWPEDGGPFLTLPLVYTEHPER
                     RESNLGMYRIQIHGPRTTGVHWQIGKGGGFHYHAAEARGEALPLTIFLGGPPALILGA
                     IAPLPEGVPELLVASLLLGRKLEMADAAGAPHRLVARAEFAITGRVPPKLRRPEGPFG
                     DHYGYYSLEHDYPVLEVDRVFRRTDAIFPATVVGKPRQEDFYIGDTLQELLSPLFPLV
                     MPSVVSLWSYGDTGFHSLAAAVVRDRYGREAMASAFRILGEGQLALTKFLLVTDARRD
                     LRDFKGLLAHVLARCRFETDLFVFGNLAMDTLDYTGPKVNEGSKGVLLGLGDAIRDLP
                     AEFRAALPAGAAAAAVFCPGALVVQGPGFGADRDYPSRLAADPAVASWPLVVLADDAA
                     ETARDERAFLWRVFTRFEPAADVHGATRVVRNHLAFSPPVVIDARTKPWMPRVVEADP
                     STVSLVDRRWREYFPKS"
     gene            complement(2333..3298)
                     /gene="pip"
                     /locus_tag="PLB01_10285"
     CDS             complement(2333..3298)
                     /gene="pip"
                     /locus_tag="PLB01_10285"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HQQ77732.1"
                     /translation="MSHPVKDPVSWLYPAIEPNRTGMLKVSDVHTLYWEESGNPKGKP
                     AVFLHGGPGGGSDPKVRRFFHPDKYRIVVFDQRGCGKSTPYASLEANTTWDLVSDIEK
                     LREHLGIAKWQVFGGSWGSTLALAYAEKHPDRVTELVLRGIFLLRKKEIDWFYQEGAS
                     IIYPDAWEPYFAHIPEAERGDLVTAYHKRLTSDDPAVRLAAAKIWSGWEGATSKLLPD
                     ASFAGHYEEDEFALAFARIEAHYFANKGFFEVDGQLLRDAGKIRHIPGVIVQGRYDVV
                     CPIFSAWALHRAWPEADLIISPDAGHSAWEPPNTRALVAATDKFA"
     gene            complement(3480..3740)
                     /locus_tag="PLB01_10290"
     CDS             complement(3480..3740)
                     /locus_tag="PLB01_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQQ77733.1"
                     /translation="MFFSRAQEIALEALPLLDPRGPAGKGRGVKDLAAASGIPAPFLA
                     KILGLLVDRGLLRSRRGRTGGFVLGRPASEIALADVVLALGR"
     gene            complement(3741..4130)
                     /locus_tag="PLB01_10295"
     CDS             complement(3741..4130)
                     /locus_tag="PLB01_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQQ77734.1"
                     /translation="MADWLVKTEPGEYSFEELRTEKETIWTGVKNPVALKHLRSMKKG
                     DRVIVYHTGGEKAAVGTATVAAAEEIPKNPAEPRVAFRAGKPLPRPIPLSEIRNCSLF
                     SDSPLVRIGRLSVVPLTSAQYAFLAKG"
     gene            complement(4142..5320)
                     /locus_tag="PLB01_10300"
     CDS             complement(4142..5320)
                     /locus_tag="PLB01_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ77735.1"
                     /translation="MLGIPLKYNVRNLFVRKVTTSLTVLGIGLVVAVFLCVMALGEGL
                     TRVFTASGSDSNVLVLRQNSQSELQSGISRDQVPIITTLPGIAPDADGKPLASQELVV
                     VLNLEKLDGGSSNVTIRGIGEKGMKLRPGLTLIEGRWFTPGKSEVVVSKSVSKRFKDC
                     ATGQTIHFGAYRWTVVGLFDAGGTAPDSEIWTDVEGMVTDFKRGGYSSVLAKTSGRAA
                     RDQFVAGLAGDPRLALEGKVERKYYDDQTSTAAPIKFLGFFVGIVMAIGACFGAMNTM
                     YAAVAARTREIATLRALGFSRISVLASFVFESLCLALLGGIVGCFLGLLAVKLALSGV
                     TGTTNFATFSEVVFAFRLTPALLGTGIVFSLVMGFFGGLFPAARAAFTKITNALRQVG
                     "
     gene            complement(5333..6490)
                     /locus_tag="PLB01_10305"
     CDS             complement(5333..6490)
                     /locus_tag="PLB01_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQQ77736.1"
                     /translation="MKFLPLVLKNLLRKKTRSGLTIGSILLPFFVICLLGTFVAMLDA
                     DPSMGKGMFRIAVRHKVSFANVLPASHLEKIRQMPGVKAAMPFNWFGGRYVDFSAFHV
                     FQRFAVDPVVFFDIFDASAIVAGSAEAWKADRSGLLVGEILMKKYGWTVGQQITLTGD
                     IWPGVYTFTIRAVYKGQNEASVFFDQKVIDEALPKAAGMYTMIWLKAVDAGATRELIP
                     RINAAFENSPWPVRAETEKEFQNNYVALLGNVKLFFRTLTAVIATVVLLIAANTMAMS
                     ARERVTEIAVLRAIGFPRRTLFGILLSESIVLALFGAALGLGIYVAAFPRLRAAVMMT
                     PVASLAAALRLYPDVLAGAFLVTILVGILAGLVPAIRSVRRPITDGLRLVA"
     gene            complement(6490..7650)
                     /locus_tag="PLB01_10310"
     CDS             complement(6490..7650)
                     /locus_tag="PLB01_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ77737.1"
                     /translation="MKFFPYVFKSLFRKKTRSLLTIASIVLPLFVICIMGTLLRTLDG
                     DPSGGRGMFRLIVRHKVSLTNWVLESYMPKIAQLRGVTDVVRMNWFGGAYIDQKPENM
                     FARFSTSDPGTLLKVFDEAAIVQGSAKDWIDDRTGLLAGELLMKKYGWKLGQKVTLKG
                     DIYPVNLELTIRATFKGPDETGVYFHHQYIEEALPRVKGFVGWYWIKADSLAAVERLP
                     GEIDAMFDNSSVPTRTETEKEMQNGFVSMLGNVKLMVTGISTVIVLVILLIAGNTMAM
                     AARERVTEIAVLRTLGYPKGTILGMMLGESVLLALVGGTVGAAIFVVLFPGFRQGLLY
                     SPMGGFAAGMKLFPEIVALAFGISVLVGVLAGLVPAIRSSRRSITDGLRQVG"
     gene            complement(7667..8407)
                     /locus_tag="PLB01_10315"
     CDS             complement(7667..8407)
                     /locus_tag="PLB01_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ77738.1"
                     /translation="MATPLIQIDSLVKQYTRDTQVLTVLDKLSLSVDDGDFVALMGPS
                     GSGKTTLLNLIAGIDRPTSGRILVGDAEVSKMSEAALAKWRSANVGFIFQLYNLVPVL
                     TAYENVELPLLLTNLNRARRRKQVELALSVVGLADRMDHYPRQLSGGQEQRVAIARAI
                     ATDPKLLVADEPTGDLDAKSGEEILILMERLNDEFKKTIVMVTHDPKAASRAHRLVHL
                     DKGVLKEDVHAEKGKAVGMAAAISASAT"
     gene            complement(8436..9641)
                     /locus_tag="PLB01_10320"
     CDS             complement(8436..9641)
                     /locus_tag="PLB01_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ77739.1"
                     /translation="MEPINKSADLTGLRIDRADEPSSRRFLWAGVATACVAAVVVLFF
                     VARSKGVAIRPPEVATTRVAIVTPTQASTVLTASGYVVARSKAEISPKSVGRVSWLNL
                     EEGQRVKKGELVARLESQELEAQKRQYVASRDQVLAQLENARRERDRAKSLLDQKIGS
                     QQAFDAADSQVRALTAQIAAAEQQVRYTDELIKNAEIYAPIDGVVTVKKAFLGETVAP
                     QGFGGAGSAGATFAVIVDLSSLEMEADINEQNVAKLSIGQPAEVALDAYPDKPYKARL
                     RQIVPTADRQKGSVKVKIEIFGKDAKVLPEMSCRVVFLNPEAKVDEKAKPKVMVPSAS
                     VVTIGSEKGVLVVANEKAVFRPLVLGAASGPQVEVTSGVSGGEEIVADAAAATHAWRR
                     EQNVRLKKD"
     gene            complement(9745..>11667)
                     /locus_tag="PLB01_10325"
     CDS             complement(9745..>11667)
                     /locus_tag="PLB01_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQQ77740.1"
                     /translation="GAASLGAGEWRQLPAPGWFGGAAVPAASRFEIAVSAGSLDAYAS
                     QIDNGTGDPVVFAPAAFPSACGPPAILAFSASATSVAPGAAVTLTLASAGGSSARVTS
                     PAGDLAIAPNGTVTVSPGTTTTYRATVSGACAPDAAASVTVTVSPQTDVALTTEGAYR
                     GTYDESAIAFKGVRYAAPPVGPLRWRPPVGPEPFPGVQAATAAGSTCLQFQDSGAGPL
                     EGTEDCLFLNVWRPAAPAASPAPVLFFIHGGGNVQGSGSEFVYDGAHLASRQGVVVVT
                     LNYRLGALGFLAQPFLDAESPRRVSGNYGILDQIAALGWVRRNAAAFGADPARILIFG
                     ESAGAVDVCTLVASPLARGLFASALMQSGGCGQETLAKVEVFGNTIVQAAGCGAAADP
                     GACMRGLPASAVVSAVPGLANVVSSSGQLYGPNVDGWLLRESPMAALTNGTHNHVPFA
                     VGANADETSAYAPTLVSDAQYQAAVVAQFGALLGALVLNRYPASAYPTPTKAYVAVTT
                     DSRFVCPSRRIARAARASQAENVFRYFFTHALDSGAKRTLGAYHGLELPFVFRSLTDV
                     PGFVPSPLELSLADAMGGYWSRIAAAGDPNGAGAPSWPRYEVASDPVIQLDDVVTSGA
                     GVRTANCDFWDAFVAP"
BASE COUNT         1809 a         3892 c         4251 g         1715 t
ORIGIN      
        1 tcggcggcgg gcgagggcgc gtggcccgcg ggggcgccct cgaacttttc gatgacgtcc
       61 tcgagcaccg ggtcgggcgc gacgaagatt tccacggaga aaccggtcgc gatcgagagg
      121 ttcttctgca ggacgatgtt ctgcgggtcc gagatcgcga tgttcaggat cttccccgac
      181 agcgagaacg ggaacgcgcg gaagcgccgc cgctccttcg cgggcaggca ctcgacgatg
      241 tccgggtcgg ccgagagcag cgtcgcgcgg gaaacgccgg gcagcccgta ggcctgggcg
      301 agcgccgacg tcagcgtctc ctcgctgacg gcgccggtcg agaggagcgc gcgctcgatc
      361 gttcccccgt gcgtcttctg ccacgtggac gcacggctca ggcggcggac cgtgaggtcg
      421 ccggcttcgg cgagcaggtc tccgactccc aggtttacgg gcatgtccgc ccatcgactt
      481 gcacgatccg ggccgggcgc tgcgggacgg aaaccgccga aacgtgtcgc tcggggccgt
      541 cccgcggggc ctttcggcgg ccgcagcccg acagaaactg tcggtcagct cttcgggaag
      601 tattcgcgcc agcgccggtc cacgagcgag accgtcgacg gatcggcctc cacgactctc
      661 ggcatccacg gcttcgtccg ggcgtcgatc acgaccggcg gcgagaacgc gaggtgattc
      721 cggacgaccc gcgtcgcgcc gtggacgtcc gccgccggct cgaagcgcgt gaagacgcgc
      781 cacaggaacg cacgctcgtc ccgcgccgtc tccgccgcgt cgtcggcgag gacgacgagc
      841 ggccacgagg cgacggccgg gtcggccgcg agacgcgacg ggtaatcgcg atccgcgccg
      901 aagcccggac cctgcacgac gagcgcgccg ggacagaaga cggcggcggc cgccgccccg
      961 gccgggaggg cggcacggaa ctccgcgggg aggtcgcgga tcgcgtcgcc gaggccgagg
     1021 aggacgccct tgctgccctc gttcaccttc gggcccgtgt agtcgagcgt gtccatcgcg
     1081 aggttcccga agacgaagag atccgtctcg aaccggcagc gcgcgaggac gtgcgcgagg
     1141 agccccttga agtcgcggag gtcgcgccgc gcgtccgtca cgaggaggaa cttcgtgagc
     1201 gcgagctggc cttcgccgag gatccggaac gccgaggcca tcgcctcgcg cccgtagcgg
     1261 tcgcgcacga cggcggccgc gagcgagtgg aagcctgtgt cgccatagct ccaaaggctc
     1321 acgacggacg gcatcacgag cgggaagagc ggcgagagca gctcctggag cgtgtccccg
     1381 atgtagaagt cctcctgccg cggcttgccg acgaccgtgg cggggaagat cgcgtccgtc
     1441 cggcggaaga cgcggtccac ctcgagaacg gggtagtcgt gctcgaggga gtagtagccg
     1501 tagtggtcgc cgaacggtcc ttcgggccgg cgcagcttcg gcggcaccct gcccgtgatc
     1561 gcgaactcgg cgcgcgcgac gagccggtgc ggcgcgcccg cggcgtcggc catctcgagt
     1621 ttcctgccga ggaggagcga ggcgacgagg agctcgggca cgccctcggg gagaggcgcg
     1681 atcgcgccga ggatcagcgc gggcggtccg ccgagaaaga tcgtgagcgg cagcgcctcg
     1741 cccctcgcct ccgccgcgtg gtagtggaaa ccgccgccct tgccgatctg ccagtggacg
     1801 cccgtggtcc gcgggccgtg gatctggatg cggtacatcc cgaggttgga ttcgcgccgc
     1861 tcggggtgtt ccgtgtagac gaggggaagc gtgaggaacg ggccgccgtc ctccggccac
     1921 tgcgtgagcg cgggcaggcg ggtgaggtcc ggcgccgtct cgacgacgtc gaggaccggc
     1981 gcgttgcggg cgcgggtcgt tccgacgcgc agcgcctcgc cgaagaacga gcggaacttc
     2041 cagagcgaag cgggcgtcgg cgggacgagc tccgtcgcgg cgcgcgcggc ggtcctcacg
     2101 aactcgagcg gccgcccgcc gaacgcgagc gcgaggcgct tcggcgtgcc gaacaggttc
     2161 gtcacgacgg ggaaatcgga gcccttcggc cgcgcgaaga ggagggcggg gccgccttcc
     2221 tcgatcacgc gccggtggat ctcggggatc tcgaggcgcg ggtcggcttc ggcctcgacg
     2281 acggcgaggt cgttctccct ccggagcgcg tcgaggaacg cccgcaggtc tctcaggcga
     2341 acttgtccgt cgcggcgacg agggcgcggg tgttcggcgg ctcccaggcg ctgtggcccg
     2401 cgtcgggcga gatgatgagg tccgcctcgg gccacgcgcg gtggagcgcc cacgcgctga
     2461 agatcgggca gacgacgtcg tagcgcccct gcacgatgac gccggggatg tggcggatct
     2521 tcccggcgtc gcggagcagc tgcccgtcga cctcgaagaa gcccttgttc gcgaagtagt
     2581 gggcctcgat gcgcgcgaag gcgagcgcga actcgtcctc ctcgtagtgg ccggcgaacg
     2641 aggcgtcggg caggagcttg gacgtcgcgc cttcccagcc gctccagatc ttcgcggccg
     2701 cgagccgcac cgccgggtcg tcgctcgtga ggcgcttgtg gtacgccgtg acgaggtcgc
     2761 cgcgctcggc ctcggggatg tgcgcgaaat acggctccca cgcgtccggg tagatgatcg
     2821 acgcgccctc ctggtagaac cagtcgatct ccttcttgcg cagcaggaag atgccgcgca
     2881 ggacgagctc cgtcacgcgg tccggatgct tctcggcgta cgcgagcgcg agcgtggatc
     2941 cccacgagcc gccgaagacc tgccatttcg cgatcccgag gtgttcgcgc agcttctcga
     3001 tgtcggagac gaggtcccag gtcgtgttcg cctcgaggct cgcgtacggc gtggacttcc
     3061 cgcagccgcg ctggtcgaac acgacgatgc ggtacttgtc cgggtggaag aagcggcgca
     3121 ccttcgggtc cgaaccgcct ccggggccgc cgtggaggaa cacggcgggt ttgcctttcg
     3181 ggtttccgct ctcttcccag tagagcgtat ggacgtcgga gaccttgagc attcccgtgc
     3241 gattcggctc gatcgcgggg tacagccagc tgacggggtc cttgaccgga tgcgacatgc
     3301 gaacctccgg ccgcatcctg ccagagtccg gggccctgcg cgcgtcaggc ccgcggggcg
     3361 gggggggcga ggtcggcgag gctcgtcgac cggagccgcg cgaggaagtc acgccgcacc
     3421 gggaccagga ggcggcccgc cgcgccgggc ggctcgggga acgcggcctc gaggtcgccc
     3481 taccttccca gtgcgaggac gacgtccgcg agcgcgatct cggaagccgg tcgtccgagg
     3541 acgaagcctc ccgtccggcc cctcctgctg cgcaggaggc cgcggtcgac gaggagcccg
     3601 aggatcttcg cgaggaacgg cgccggaatg cccgaggccg ccgcgaggtc cttcacgccg
     3661 cggcccttcc cggcggggcc gcgcggatcc aggaggggca gcgcctcgag ggcgatctcc
     3721 tgcgcgcgcg agaagaacat tcagcccttc gcgaggaagg cgtattgcgc gctcgtgagg
     3781 gggacgaccg agaggcgtcc gatgcggacg agtggggagt cggagaagag agagcaattt
     3841 cggatttctg agagtggaat agggcgaggg agaggttttc ccgcgcgaaa ggcgactcgc
     3901 ggttccgccg ggttcttggg gatttcctct gcggcggcca cggtcgcggt gcccacggcg
     3961 gccttctcgc cgcccgtgtg gtagacgatc acgcggtcgc ccttcttcat cgagcgcagg
     4021 tgcttgagcg ccacgggatt cttcacgccc gtccagatcg tctctttttc cgtgcgcagt
     4081 tcctcgaacg agtattcccc gggctccgtc ttcacgagcc agtcggccat gtgtcgcctc
     4141 ctcagcccac ctgccgcaac gcgttcgtga tcttcgtgaa tgccgcccgg gcggccggga
     4201 agaggccgcc gaagaaaccc atcacgagcg agaagacgat gcccgtgccg aggagcgccg
     4261 gcgtgaggcg gaacgcgaag acgacctcgg agaacgtcgc gaagttcgtc gtgcccgtga
     4321 cccccgagag cgcgagcttg acggcgagga ggccgaggaa gcagccgacg atcccgccga
     4381 ggagcgcgag gcagagggac tcgaagacga acgaggcgag gaccgagatc cgcgagaagc
     4441 cgagcgcccg gagcgtcgcg atctcccgcg tccgcgccgc cacggccgcg tacatcgtgt
     4501 tcatcgcgcc gaagcacgcg ccgatcgcca tgacgatccc gacgaagaag ccgaggaact
     4561 tgatgggcgc cgccgtggag gtctggtcgt cgtagtactt gcgctcgacc ttgccctcga
     4621 gcgcgaggcg gggatcgcct gcgaggccgg cgacgaactg gtcgcgagcc gcgcggccgg
     4681 acgtcttcgc gaggaccgac gagtagccgc cccgtttgaa gtccgtgacc atcccttcga
     4741 cgtccgtcca gatctcgctg tcgggcgcgg tgccgccggc gtcgaagagc ccgacgacgg
     4801 tccagcggta ggctccgaag tggatcgtct ggcccgtcgc gcagtccttg aagcgcttgg
     4861 aaacgctctt ggagacgacg acctccgact tgccgggcgt gaaccagcgg ccctcgatga
     4921 gcgtgaggcc gggccgcagc ttcatcccct tctcgccgat cccgcggatc gtcacgttcg
     4981 aggagccgcc gtcgagcttt tcgaggttca ggacgacgac gagctcctgc gacgcgagcg
     5041 gcttgccgtc cgcgtccggc gcgatgccgg ggagcgtcgt gatgatcgga acctggtctc
     5101 gcgagatgcc cgattgcagc tcggactggg agttctgcct gaggacgagg acgttggagt
     5161 ccgatcccga tgccgtgaaa acgcgggtga gcccctctcc gagcgccatg acgcagagga
     5221 agaccgcgac cacgagcccg atcccgagga ccgtgagaga cgtcgtcacc ttgcggacga
     5281 agaggttgcg gacgttgtac ttcagcggga tgccgagcat cgggggctcc tcctacgcga
     5341 cgagacgaag gccgtccgtg atggggcggc ggacggaccg gatggcgggg acgaggccgg
     5401 cgagaatgcc gacgaggatc gtgacgagga acgcgccggc gaggacgtcg gggtagaggc
     5461 gcagggcggc ggcgagcgag gcgactggag tcatcatgac cgcggcgcgc agacgcggga
     5521 aggccgcgac gtagatcccg aggccgagcg ccgcgccgaa gagcgcgagg acgatgctct
     5581 cggacagcag gatcccgaag agcgtgcgcc gcgggaagcc gatggcgcgc aggaccgcga
     5641 tctccgtcac gcgctcccgc gccgacatcg ccatcgtgtt cgcggcgatc aggagcacga
     5701 cggtcgcgat gacggcggtg agggtccgga agaagagctt cacgttgccg aggagcgcca
     5761 cgtagttgtt ctggaactcc ttttcggtct cggcccgcac cggccagggc gagttttcga
     5821 aggcggcgtt gatccgcggg atcagctccc gcgtcgcccc ggcgtcgacg gccttgagcc
     5881 agatcatcgt gtacatcccc gcggccttcg ggagcgcctc gtcgatgacc ttctggtcga
     5941 agaacacgga ggcctcgttc tggcccttgt agacggcccg gatcgtgaac gtgtaaaccc
     6001 ccggccagat gtcgccggtg agcgtgatct gctggccgac cgtccagccg tacttcttca
     6061 tcaggatctc gcccacgagg aggccgctcc ggtcggcttt ccacgcctcg gccgagccgg
     6121 ccacgatcgc ggacgcgtcg aagatgtcga agaagacgac cgggtcgacc gcgaagcgct
     6181 ggaagacgtg gaacgccgag aagtcgacgt accggccgcc gaaccagttg aacggcatcg
     6241 cggctttcac gccgggcatc tgccggatct tctcgaggtg ggacgcgggg aggacgttcg
     6301 cgaaagacac cttgtgccgc accgcgatcc ggaacatgcc cttgcccatg gacgggtcgg
     6361 cgtcgagcat cgcgacgaac gttccgagaa ggcagatcac gaagaagggc agcaggatcg
     6421 agccgatcgt cagacccgag cgcgtcttct tgcgcagcag gttcttcagg acgagcggca
     6481 ggaacttcat cagccgacct gtctcaggcc gtccgtgatg gagcgtcgcg acgagcggat
     6541 ggcggggacg agtccggcga ggaccccgac gaggacggag atcccgaacg cgagagcgac
     6601 gatttccggg aagagcttca tgccggccgc gaagccgccc atcggagagt agagaagtcc
     6661 ctgtcggaag ccggggaaga ggaccacgaa aatcgccgcg ccgaccgtcc cgcccacgag
     6721 cgcgaggagg acgctctcgc cgagcatcat cccgaggatc gtgcccttcg ggtagccgag
     6781 cgtcctcagg acggcgatct ccgtcacgcg ctcgcgcgcc gccatcgcca tcgtgtttcc
     6841 cgcgatgagg aggatgacga ggacgatcac cgtcgagatg cccgtgacca tgagcttcac
     6901 gttcccgagc atcgagacga atccgttctg catctccttc tccgtctccg tgcgcgtggg
     6961 gacggagctg ttgtcgaaca tcgcgtcgat ctccccgggc aggcgctcga cggcggcgag
     7021 ggagtccgcc ttgatccagt accagcccac gaagcccttc acgcggggca gggcctcctc
     7081 gatgtactgg tggtggaaat agacgccggt ctcgtccggg cccttgaagg tcgcgcggat
     7141 cgtgagctcg aggttcacgg ggtagatgtc gcccttgagg gtgaccttct gcccgagctt
     7201 ccagccgtac ttcttcatga ggagctcgcc ggccaggagg cccgtcctgt cgtcgatcca
     7261 gtctttcgcg gagccctgga cgatggcggc ctcgtcgaag accttcagga gagtcccggg
     7321 gtccgacgtc gagaagcgcg cgaacatgtt ctcgggcttc tggtcgatgt acgccccgcc
     7381 gaaccagttc atgcggacga cgtcggtgac gccgcggagc tgcgcgatct tcggcatgta
     7441 ggactcgagg acccagttcg tcagcgagac cttgtgccgc acgatgagcc ggaacatccc
     7501 cctgccgccc gacgggtcgc cgtcgagcgt ccggagcagc gttcccatga tgcagatgac
     7561 gaagagcggc aggacgatcg acgcgatcgt caggagcgag cgcgtcttct tgcggaacag
     7621 gctcttgaag acgtaaggga agaacttcac cgggcctccg tggagctcag gtggccgacg
     7681 ccgagatcgc ggcggccatg ccgacggcct tgcccttctc ggcgtggacg tcctccttca
     7741 ggacgccctt gtcgaggtgg acgagccggt gggcgcgcga cgcggccttc gggtcgtgcg
     7801 tgaccatgac gatcgtcttc ttgaactcgt cgttgagccg ctccatgagg atcaggatct
     7861 cctcgccgct cttcgcgtcg aggtcgcccg tcggctcgtc cgcgacgagg agcttcgggt
     7921 cggtcgcgat ggcgcgcgcg atcgcgacgc gctgctcctg cccgcccgag agctggcgcg
     7981 ggtagtggtc catgcggtcc gcgaggccga cgaccgagag cgcgagctcg acctgcttcc
     8041 ggcggcgcgc gcggttcagg ttcgtgagga ggagcggcag ctcgacgttc tcgtaggccg
     8101 tcaggacggg cacgaggttg tagagctgga agatgaaccc gacgttcgcg ctccgccact
     8161 tcgcgagcgc cgcctcgctc atcttcgaga cctccgcgtc cccgacgagg atgcgccccg
     8221 aggtcgggcg gtcgatcccg gcgatgaggt tcaggagcgt cgtcttgccc gaccccgacg
     8281 gccccatgag cgccacgaag tccccgtcgt ccacggacaa cgagagcttg tcgaggacgg
     8341 tgagcacctg cgtgtcgcgg gtgtattgct tgacgagcga gtcgatctgg atgagagggg
     8401 tcgccatgcg gctctccttc gtgtccccgg ggggatcagt ctttcttgag gcggacgttc
     8461 tgctcgcggc gccacgcgtg cgtcgcggcg gcggcgtcgg cgacgatctc ctcgccgccc
     8521 gagaccccgg atgtcacctc gacctgcgga ccggaggcgg cgccgaggac gagcggccgg
     8581 aagacggcct tctcgttcgc gacgacgagg acgcccttct cgctcccgat cgtcacgacc
     8641 gaggccgacg gcaccatgac cttcggcttc gccttctcgt cgaccttcgc ctcggggttc
     8701 aggaagacga cgcggcagga catctcgggc agcaccttcg cgtccttgcc gaagatctcg
     8761 atcttgacct tcacggagcc cttctgccgg tcggcggtcg ggacgatctg cctgaggcgc
     8821 gccttgtacg gcttgtcggg gtacgcgtcg agcgcgactt cggccggctg gccgatcgag
     8881 agcttcgcga cgttctgctc gttgatgtcg gcctccatct cgagggagga caggtcgacg
     8941 atgacggcga acgtcgcgcc cgccgagccg gcgccgccga agccctgcgg ggcgaccgtc
     9001 tcgccgagga acgccttctt cacggtgacg acgccgtcga tcggcgcgta gatctcggcg
     9061 ttcttgatga gctcgtccgt gtaccggacc tgctgctcgg ccgcggcgat ctgggccgtc
     9121 agcgcgcgca cctgcgagtc cgcggcgtcg aacgcctgct gcgagccgat cttctggtcg
     9181 aggagggact tggcgcggtc gcgctcgcgg cgcgcgttct cgagctgcgc gaggacctgg
     9241 tcgcgcgacg cgacgtactg gcgcttctgc gcctccagct cctgcgactc gaggcgggcc
     9301 acgagctcgc ccttcttcac cctctggcct tcctcgaggt tcagccacga gacgcgcccg
     9361 acggatttcg gggagatctc ggccttcgag cgcgcgacga cgtaacccga cgccgtgagg
     9421 acggtcgagg cctgcgtcgg cgtcacgatc gcgacgcgcg tcgtcgcgac ctcgggcggc
     9481 cggatcgcga cgcccttgct gcgggccacg aagaagagga ccacgacggc cgcgacgcac
     9541 gcggtcgcga cgcccgccca gaggaagcgc cgggaggacg gttcgtcggc gcggtcgatg
     9601 cggaggcccg tgaggtcggc ggatttgttg atgggttcca taagcaattc agactcggca
     9661 gtacgatatc cgaagcgcga tcgtttcagg atggggggcg gttttcggcg aacgggcggc
     9721 cccgaccggc gaacggcggc gctctcaggg cgcgacgaac gcgtcccaga aatcgcagtt
     9781 cgccgtccgg accccggctc cggaggtcac gacgtcgtcg agctggatca ccgggtcgga
     9841 cgcgacctcg tagcgcggcc aggagggcgc gcccgcgccg ttcgggtccc ccgcggccgc
     9901 gatccggctc cagtagccgc ccatcgcgtc cgcgagagag agctcgagcg gcgaggggac
     9961 gaagcccggc acgtccgtga gcgaccggaa cacgaacggg agctccaggc cgtggtacgc
    10021 gccgagcgtg cgcttcgccc ccgaatcgag agcgtgggtg aagaagtagc ggaagacgtt
    10081 ttcggcctgg ctcgcgcgcg cggcgcgtgc gatccgccgc gaggggcaca cgaagcgtga
    10141 atccgtcgtc acggcgacgt aggccttcgt cggggtcggg taggcgctcg cggggtagcg
    10201 gttcagcacg agcgccccga gcagggcccc gaactgggcg acgaccgcgg cctggtactg
    10261 cgcgtccgaa acgagcgtcg gcgcgtaggc ggacgtttcg tccgcgttcg cgccgacggc
    10321 gaaaggcacg tggttgtgcg tgccgttcgt gagggcggcc atggggctct cccggagcag
    10381 ccagccgtcc acgttcggcc cgtagagctg gcccgagctc gagacgacgt tcgcgaggcc
    10441 cggcaccgcc gacacgacgg cagacgcggg aagtccccgc atgcacgcgc ccgggtcggc
    10501 cgccgcgccg cagcccgcgg cctggacgat cgtgttcccg aacacctcga ccttcgcgag
    10561 cgtctcctgt ccgcagcccc cgctctgcat gagcgcgctc gcgaagagtc ccctcgcgag
    10621 cggggacgcg acgagcgtgc agacgtccac ggcgccggcc gactcgccga agatgagaat
    10681 cctcgcggga tcggcgccga acgccgcggc gttgcgccgg acccacccga gcgccgcgat
    10741 ctggtcgagg atcccgtagt tgcccgacac gcggcgcggg ctctcggcgt cgaggaaggg
    10801 ctgtgcgagg aagccgagcg cgccgagccg gtagttcagc gtcacgacga cgacgccctg
    10861 gcgcgaggcc aggtgcgctc cgtcgtagac gaactcgctc ccggacccct ggacgttccc
    10921 gccgccgtgg atgaagaaga ggacgggcgc cggagaagcc gccggtgcgg cgggcctcca
    10981 gacgttcagg aagaggcagt cctctgtgcc ctccagcggg cccgcgcccg agtcctggaa
    11041 ctggaggcac gtgcttcccg ccgccgtggc ggcctggacg cccgggaatg gctccggccc
    11101 cactggaggc ctccagcgca ggggtccgac gggcggcgcc gcgtagcgga cgcccttgaa
    11161 cgcgatggcg ctctcgtcgt aggtcccccg gtacgccccc tcggtcgtga gcgcgacgtc
    11221 ggtttgcggg gaaaccgtca cggtcacgga cgccgcggcg tcgggtgcgc aggcgcccga
    11281 gaccgtcgcg cggtacgtcg tcgtggtgcc gggtgagacg gtgaccgttc cgttcggcgc
    11341 gatcgcgagg tctcccgcag gggacgtcac gcgagccgac gaaccacccg cggaggcgag
    11401 cgtcagcgtc acggccgcac ccggcgcgac ggacgtcgcc gacgcggaga aggcgaggat
    11461 ggccggcggt ccgcacgccg acggaaatgc cgcgggagcg aaaaccacgg ggtcccccgt
    11521 cccgttgtcg atctggctcg catacgcgtc gagcgagccc gcggagacgg cgatctcgaa
    11581 ccggctcgcc gcggggaccg ccgctccgcc gaaccagccg ggggccggga gctgccgcca
    11641 ctcgccggcg ccgagcgacg cggcccc
//
LOCUS       DAOMFY010000108        11604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1728_length_11604_cov_6.997835, whole genome shotgun sequence.
ACCESSION   DAOMFY010000108 DAOMFY010000000
VERSION     DAOMFY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11604
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_11604_cov_6.997835"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLB01_10330"
     CDS             <1..207
                     /locus_tag="PLB01_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77741.1"
                     /translation="AAPADAIALVRDAAALRLTKPGGAGAVREMCDLLIEVRRGARGD
                     KPAAVEPEKTAEDGDVIPFPFGRN"
     gene            237..707
                     /gene="nrdR"
                     /locus_tag="PLB01_10335"
     CDS             237..707
                     /gene="nrdR"
                     /locus_tag="PLB01_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQQ77742.1"
                     /translation="MRCPYCGEGDDRVVDSRESREGAVIRRRRECNACQRRFTSYETV
                     EEIPLLVVKKDGRREPFDRNKLLRGLMKACEKRPVETKTLEEICDAVESELSAREPRE
                     MTTIELGASIMEKLKDLDQVAYVRFASVYRRFEDLGDFVDELKALLGRAPGGKK"
     gene            704..1105
                     /locus_tag="PLB01_10340"
     CDS             704..1105
                     /locus_tag="PLB01_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQQ77743.1"
                     /translation="MKKREIPVGFFVRTAHEPPERPALVPPVDVVDHGSLWRLTFEIP
                     GTDPEEISVDIRGRLLTLRGTRKATDRESGVFLRVERAAGAFERVLELPEDPDPEGSR
                     ATYADGLLMLDIPKRSGGKGRSIPIVKGKKS"
     gene            1105..3534
                     /gene="lon"
                     /locus_tag="PLB01_10345"
     CDS             1105..3534
                     /gene="lon"
                     /locus_tag="PLB01_10345"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQQ77744.1"
                     /translation="MSSVDPGANAQVAIPDILPVLPLKDVVLFPYVIVPLSVSREKSI
                     AAVDAALAEQRVLLLVAQRDASVEEPTASDLYTIGTAAAVMRMLKLPDGRIRLLVQGL
                     SRVKIESILADSPSLRAKVTRIPEPDAQTPPPEEVEALVRSAKESLEKTVALGKSISP
                     EVMVVAAGLDEPARLADLAASNLELKIDEAQKILETIPAVERLKTVLEILRREVRVLT
                     VQQEISGAAKGEIDKSQREYFLRQQLKTIQQELGETEDLSEEIAAYRAKIAEKQIPEE
                     AAQEIEKQVKRLERGNPDSAENAMLRTYLDWLVNLPWSVVSTDSLDLSRAKKILDEDH
                     YDLEKVKERILEFLAVRKLSPESKGPILCFVGPPGVGKTSLGRSIARALDRKFLRLSL
                     GGVRDEAEIRGHRRTYVGAMPGRIIQGLAQAGTSNPVYILDEVDKIGADFRGDPSSAL
                     LEVLDPEQNKTFRDHYLGVNYDLSKVLFITTANVLDTIQPAFLDRMEVIRLSGYTTEE
                     KLAIAKAHTVPKQRKENGLPDGAVTFKDDALRAIIENYTREAGLRSFEREIGSCCRKV
                     AVRWASGKKKPVVVTPREVKAFLGIPKFTRDEALRTDQVGTATGLAWTAVGGDVLIVE
                     ARAVRGKGRLQLTGQLGDVMKESAQAALTFARSRADRYGLAPDYFETHDVHIHVPEGA
                     IPKDGPSAGVTMATAMISAFGGTRVRRDVAMTGEITLRGDVLPIGGVKEKVLAARRAG
                     IGTVILPKANRRDLAEIPKELRRNLKFVFAARVDDVLGAAFIGPAAARKKTAPGRAAT
                     TAAARKPGKAA"
     gene            3531..4571
                     /gene="thiL"
                     /locus_tag="PLB01_10350"
     CDS             3531..4571
                     /gene="thiL"
                     /locus_tag="PLB01_10350"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HQQ77745.1"
                     /translation="MKRRSGLPPEDAIVARLAGVFATRRRDVKVGIGDDAAVVVSPDR
                     LAVTTDVLLEDHDFRRDADPRRLGRKAMNVNLSDLAAMGARPLWAVLALGLPKKTKPE
                     WLDALAAGVKEAASEHGVAVVGGDLSASDKVFVAVTAMGHAPEAPLTRAGAEPGDALF
                     VSGTLGAAAAGFRLLEKGYRLSAERVALDPKGRRVSAPRAQEVARLIRHQIDPRPQLV
                     LGSTLASGSLASAAIDVSDGLARDLYRLCRASDVGATIEADRLPVDSALADLGPLAGL
                     DPLAAALYGGEDFGLLFTVPKKKLAAVDRLAGSFALRRIGTIDDGAGVRLVRDGRRAP
                     LPDAGFDHFDEP"
     gene            4558..5070
                     /locus_tag="PLB01_10355"
     CDS             4558..5070
                     /locus_tag="PLB01_10355"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HQQ77746.1"
                     /translation="MTNPEGSFKARFLHMLGRDSPPEVVAASFAIGVAISFTPLIGLH
                     WVIALLLAFALKLNKVDVLLGTLVVNPLTLPAVGAIALPIGRFLLQARREAIAHLPWH
                     EMFRRSFWTAAGPTMRAVGAQIAVGMFALSLVAGALTYVVLLRLIKRHRAKLAAKAAP
                     DVAGEGVEPD"
     gene            complement(5067..5726)
                     /locus_tag="PLB01_10360"
     CDS             complement(5067..5726)
                     /locus_tag="PLB01_10360"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ77747.1"
                     /translation="MALRFGDCLFDPESRQLFRGGAPVPLSPKAFHLLEILVRNRPRA
                     MAKAELQETLWPDTFVVEANLANLVAELREAMGEKGHRDGFLRTVHGFGYAFSGEAAD
                     SGVRAAHASAFSLVRDGRAFPLAAGENVIGRDPECAVFVDDGTVSRRHACVTVRGNEA
                     LLEDLGSKNGTSVNGRKPEGPCAVRHGDSVLVGSVLFTVRVSAAAQETATLRDAPASA
                     P"
     gene            5803..7080
                     /locus_tag="PLB01_10365"
     CDS             5803..7080
                     /locus_tag="PLB01_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77748.1"
                     /translation="MSGAPAGRIPSAAAIALLLAGAFRVAGQEPTILAGEAFTRTAFG
                     MTFEVPARDRSTVDEVFAGLGWIPSGPDGKTLNPEAAFLLWRQRDGGARRLRAVLVGL
                     YDDVRWNRRIAESTDLVLTFENTTFPWARSEYASGERLESEELNWGSVRAGAGLAFRS
                     PLAPGHADNFLEGSLSYEPGFLYFSKGSSTATDFTPPSNSYEGRFHLRVRADAFTRNL
                     LELPHRGWAAGLDAWAAHRGGWQDWGGGPTGAHAAAYTRSWTALSGYALAALPLPLGD
                     RRHRILASVYAGTGSDLDRFSAFRLSGGSNAGDYETITRPVLPTAGLDEIVSRGYVIA
                     GLEARMQLAFFLFLHLRGTLASVDRTVRDASGAILQRTDSPNAVTIGLSSGFFWSSMI
                     ELSWSRNAALESREGDGFTSGRDGVYFSFTRSF"
     gene            7127..9523
                     /locus_tag="PLB01_10370"
     CDS             7127..9523
                     /locus_tag="PLB01_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77749.1"
                     /translation="MTLHAGDRLGAYEILAPLGSGGMGEVWRARDTRLKREVAIKVLP
                     DSLASDPVARTRFDREARAVAALSHPNILDIHDVGSEGGIAWAVTELLEGQTLKARLD
                     EGALPWRRAVEVALALAEGLEAAHARGVVHRDIKPSNVFLTSDGRVKILDFGIATLDD
                     STSLSLGARDVTEPHAIVGTAGYLSPEQIRREAADARSDIFSLGCVLYEMVTGRRTFP
                     GDTPPEALVATLRGEPRDPADLVTGLPPELRRLILRCLEKRPDRRFQTAADLAFALKI
                     PFTGSGGGHEFVSATDETEVIASAANASPKRRAASVAAGGALFASVLAAAAWMLFFRG
                     AGPAGNVLAVLPFTNETGDSAHEYLSEGVTDSLIDALSKTPGLSVLARSTVYLVPDRH
                     DPRKAGRFLHARTVLVGRVKRQGADLHVDAELVDVATGARLWHKIVTDRAGDPVPAAA
                     DLAPGIARTLRPALTAEEEKNVAGRRHENPEAWDRVQRGRFFLKRRTEEGIFKALGYF
                     QDAALLEPREADAWCGMAEVYHLLAYYSYAEPAELVPKEREVAAKAAALDPTLSGPHA
                     ILADIRYLTDHDWPGAEEEFRKAIALNPSDAEAHQWYSNFLSAAGRPDEALAEILRAR
                     TLDPLSAVIQADVGLARYFGGDMAGGEKEIRRAIELDPHAFLPRLWASLPLLAQKRYD
                     EALVELGKAREIERAVPEPIAFWGYASALAGRPADADAARRDLDALKKERYVGGFPYA
                     ILTLGQKKREESLDWLEKSAAAGDGRLAYLGVEPGFDPLRSDPRFTAVVRHLAIPKPR
                     "
     gene            complement(9525..>11604)
                     /locus_tag="PLB01_10375"
     CDS             complement(9525..>11604)
                     /locus_tag="PLB01_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77750.1"
                     /translation="PPRVSAGPPARLGRAGALGLAILAILPFLNGLPADFTYDDKLII
                     SGNDRIASPSKVGEVFTTQYFGGSLASAQNYRPVLLLTYGVQRWIHGNRAWLFRAVNI
                     ALHAAVTVFFAAWLVELGFASRAAIAAGALFAVFTIHVEAVTSLVGRAELLAALAVFA
                     VARLWLRATAGPRLRAGPYAGTLGVFLLSVFVKENAIVAPGVVLLGELFRGGRGRSPR
                     EAWAALPRSARWALTGFLGPIVVLFAVRVIVIHGFLVSREAGIFDLENPLVAMKPALR
                     VANALTLAGLYALKTFAPVRLAADHSAYALTLAADLAEWRAWAGVAFVVALLVLALAF
                     WRRRPLAALGIALFGGSLFPVSNVPFVIGTIWAERLAYLPSAGLAALAVGIVAGLALE
                     VPRPSRFPWREALLAAAVLAYGVAAAVRNLAWADDRTLYRDMVAKNPNSAKAHYNVAW
                     EAQLRGDRETTIRHLTRAIEIFPRYYDAWALLGKQRWDEKKWDEAIGCYQRSIQIFST
                     YENGHWGLAKVYEAAGRLEEAGREFEEGARLLPDSYPIAYHRAAFLEARGKLVEAEDA
                     WDDAVENGDGAASVRLAHARVLVKLGRESEAWDEARWALVNAPSNVDARRFLAERYEA
                     SGRTLAAGAERARAVRSNPADEASAADLLEYALREPSARVRARMLVPEIRAAVPKPGP
                     RLAKALADFG"
BASE COUNT         1745 a         4251 c         4049 g         1559 t
ORIGIN      
        1 gccgcgccgg ccgacgcgat cgcgctcgtg cgcgacgccg ccgccctgcg cctcacgaag
       61 cccgggggcg ccggggccgt gcgcgagatg tgcgacctcc tgatcgaggt gcgccgcggg
      121 gcacggggcg acaagcctgc cgccgtggaa cccgaaaaga ccgcggaaga cggcgacgtc
      181 atcccgttcc ctttcgggcg gaactgacgc cccggtcctg ctaggctcgg ggccacatgc
      241 gctgtccgta ctgcggggaa ggcgacgacc gcgtcgtcga ttcgcgagag agccgggagg
      301 gcgccgtcat ccgccggcgg cgcgagtgca acgcctgcca gaggcgcttc acgtcctacg
      361 agacggtcga ggagatcccg ctcctcgtcg tgaagaagga cggtcggcgc gagcccttcg
      421 accgcaacaa gctcctgcgg ggcctcatga aggcctgcga gaagcggccc gtcgagacga
      481 agacgctcga ggaaatctgc gacgccgtcg aatccgagct gtcggcgcgc gaaccgcgcg
      541 agatgacgac gatcgagctc ggcgcgagca tcatggagaa gctcaaggac ctcgaccagg
      601 tcgcctacgt ccggttcgcg tcggtctacc gccgcttcga ggacctcggc gacttcgtgg
      661 acgagctcaa ggcgctcctc ggacgcgccc ccggagggaa gaaatgaaga agcgcgagat
      721 ccccgtcggc ttcttcgtcc gcacggccca cgagccgccc gaacggcccg ccctcgtccc
      781 gccggtcgac gtcgtggacc acggctctct ctggcgcctc acgttcgaga tccccggcac
      841 ggaccccgag gagatctcgg tggacatccg cgggcggctc ctgacgctcc gcgggacgcg
      901 caaggcgacg gaccgcgagt ccggcgtctt cctgcgcgtc gagcgcgccg cgggcgcgtt
      961 cgagcgcgtg ctcgagctgc ccgaggatcc ggatcccgaa ggctcccgcg ccacgtacgc
     1021 ggacgggctc ctcatgctcg acattccgaa gcgttcgggc ggcaagggcc ggtcgattcc
     1081 catcgtgaaa ggcaagaaga gctgatgagc tccgtggacc ccggtgcgaa cgcgcaggtc
     1141 gcgattcccg acatccttcc ggtcctgccg ctgaaggacg tcgtcctctt cccctacgtc
     1201 atcgtgccgc tctccgtgtc gcgcgagaag tcgatcgcgg cagtcgacgc cgctctcgcg
     1261 gaacagcgcg tcctcctcct cgtcgcgcaa cgggacgcgt ccgtcgagga gccgacggcg
     1321 agcgacctct acacgatcgg gacggccgcg gccgtcatgc ggatgctcaa gctcccggac
     1381 gggcgcatcc gcctcctcgt gcaggggctg tcgcgcgtca agatcgagtc gatcctcgcg
     1441 gactccccgt cgctccgggc gaaggtcacg cgcattcccg agccggacgc ccagacgccg
     1501 ccgcccgagg aggtcgaggc gctcgtgagg agcgccaagg agtccctcga gaagacggtc
     1561 gcccttggca agtcgatctc gcccgaagtg atggtcgtcg ccgcgggcct cgacgagccc
     1621 gcgcgcctcg ccgacctcgc agcctcgaac ctcgagctca agatcgacga ggcccagaag
     1681 atcctcgaga cgatcccggc cgtcgagcga ctgaagaccg ttctcgagat ccttcggcgc
     1741 gaggtgcgcg tcctgacggt ccagcaggag atctcggggg ccgccaaggg cgagatcgac
     1801 aagagccagc gcgagtactt cctgcgccag cagctcaaga cgatccagca ggagctgggc
     1861 gaaaccgagg acctctccga ggagatcgcc gcctaccgcg cgaagatcgc cgagaagcag
     1921 attcccgagg aggccgcgca ggagatcgag aaacaggtca agcgcctcga gcgcggcaat
     1981 ccggactcgg ccgagaacgc gatgctgcgg acctatctcg actggctcgt gaacctcccg
     2041 tggtcggtcg tgtccaccga cagcctcgac ctctcccgcg cgaagaagat cctcgacgag
     2101 gaccactacg acctcgagaa ggtcaaggag cgcatcctcg agttcctcgc cgtgaggaag
     2161 ctctcccccg agtcgaaggg gccgatcctc tgcttcgtcg ggccgccggg cgtcggcaag
     2221 acgtcgctcg gccgctcgat cgcccgggcc ctcgaccgga agttcctgcg cctgtcgctc
     2281 ggcggcgtgc gcgacgaggc cgagatccgc gggcaccgcc gcacctacgt gggcgcgatg
     2341 ccgggccgca tcatccaggg tctcgcgcag gccggaacct cgaaccccgt ctacatcctg
     2401 gacgaggtcg acaagatcgg cgcggacttc cgcggcgacc cgtcgtcggc cctgctcgag
     2461 gtcctcgacc ccgagcagaa caagacgttc cgcgaccact acctcggcgt gaactacgac
     2521 ctctcgaagg tcctcttcat cacgaccgcg aacgtcctcg acacgatcca gccggccttc
     2581 ctcgaccgca tggaggtgat ccgcctctcg ggctacacga ccgaggagaa gctcgcgatc
     2641 gcgaaggccc acacggtccc caagcagagg aaggagaacg gcctgcccga cggcgccgtg
     2701 acgttcaagg acgacgcgct ccgcgcgatc atcgagaact acacgcgcga ggccgggctg
     2761 cgcagcttcg agcgcgagat cggctcgtgc tgccgcaagg tggcggtccg ctgggcttcg
     2821 ggcaagaaaa agcccgtcgt cgtgacgccc cgcgaggtga aggcgttcct cggaatcccg
     2881 aaattcacgc gggacgaggc gcttcggacc gaccaggtcg ggacggcgac gggcctcgcg
     2941 tggacggccg tgggcggcga cgtcctcatc gtcgaggcgc gggccgtccg cggcaagggc
     3001 cgcctccagc tgacggggca gctcggcgac gtcatgaagg aatccgcgca ggccgcgctc
     3061 accttcgcgc gctcccgcgc cgaccggtac ggcctggctc ccgactactt cgagacgcac
     3121 gacgtccaca tccacgtgcc cgagggcgcg attccgaagg acggcccctc ggccggcgtc
     3181 acgatggcga ccgcgatgat ctcggctttc gggggcacgc gcgtgcggcg cgacgtcgcg
     3241 atgacgggcg agatcacgct ccgcggcgac gtcctcccga tcggcggcgt caaggagaag
     3301 gtcctcgcgg cccgccgcgc gggcatcggc acggtcatcc tgccgaaggc caacaggcgc
     3361 gacctcgcgg agatcccgaa ggagctccgc cgcaacctga agttcgtctt cgccgcgagg
     3421 gtggacgacg tcctcggggc cgcgttcatc ggcccggccg cggcgaggaa gaagacggct
     3481 ccgggccggg cggccacgac ggccgccgcg aggaagccgg ggaaagccgc atgaagagac
     3541 gctccggcct cccgcccgaa gacgcgatcg tcgcccgcct cgcgggcgtg ttcgcgacac
     3601 gacgacgcga cgtgaaggtc gggatcggcg acgacgcggc cgtcgtcgtg agcccggacc
     3661 ggctcgcggt cacgaccgac gtcctcctcg aggaccacga cttccgccgg gacgccgatc
     3721 cccggaggct cgggcgcaag gcgatgaacg tgaacctctc cgacctcgcc gcgatgggcg
     3781 cgcggcccct ctgggccgtc ctcgcgctcg gcctgccgaa gaagacgaag cccgagtggc
     3841 tcgacgcgct cgcggccggc gtgaaggagg cggcttccga gcacggcgtc gccgtcgtcg
     3901 gcggcgacct cagcgcctcc gacaaggtct tcgtggcggt cacggcgatg ggccacgcgc
     3961 ccgaggctcc gctcacgcgc gcgggggccg agccgggaga cgcgctcttc gtctccggga
     4021 cgctcggcgc ggccgctgcg ggcttcaggc tcctcgagaa ggggtatcgc ctctcggccg
     4081 agcgcgtcgc gctggacccg aagggccggc gcgtttccgc cccgcgcgcc caggaagtcg
     4141 cccgcctcat ccggcaccag atcgaccccc gtccccagct cgtgctcggc tcgacgctcg
     4201 cctcgggctc cctcgcctcg gccgcgatcg acgtctcgga cgggctcgcg cgcgacctct
     4261 accgcctctg ccgggcctcc gacgtcgggg cgacgatcga ggcggaccgg ctccccgtcg
     4321 actcggccct cgccgacctc gggccgctcg cgggcctcga cccgctcgcc gccgcgctct
     4381 acggaggcga ggacttcggc ctcctcttca cggtcccgaa gaagaagctc gccgcggtcg
     4441 accgcctcgc gggaagcttc gcgctgcggc gcatcgggac gatcgacgac ggcgcgggcg
     4501 tgcggctcgt gcgcgacggg cggcgggcgc cactgcccga cgccgggttc gaccactttg
     4561 acgaaccctg aggggtcgtt caaggcccgc tttctccaca tgctcgggag ggactccccc
     4621 cccgaggtcg tcgcggcctc gttcgcgatc ggcgtcgcga tctccttcac gccgctcatc
     4681 gggctccact gggtgatcgc cctcctcctc gcgttcgccc tgaagctcaa caaggtcgac
     4741 gtcctgctcg ggacgctcgt cgtgaacccg ctgaccttgc ccgcggtcgg cgcgatcgcg
     4801 ctgccgatcg gacgcttcct gctccaggcg cggcgcgagg ccatcgccca cctgccgtgg
     4861 cacgagatgt tccggcgctc gttctggacg gccgcggggc cgacgatgcg cgcggtcggc
     4921 gcccagatcg ccgtcgggat gttcgcgctc tcgctcgtcg cgggcgcact cacgtacgtc
     4981 gtgctcctgc ggctcatcaa gcgccaccgc gcgaagctcg ccgcgaaagc cgccccggac
     5041 gtcgcgggcg agggcgtcga gccggactag ggtgcggacg ccggggcgtc tcgcagcgtc
     5101 gccgtctcct gggccgccgc cgaaacccgg acggtgaaaa gcaccgagcc caccagaacg
     5161 gagtcgccgt gccggaccgc acacgggccc tccggcttgc ggccgttcac gctcgtcccg
     5221 ttcttgctgc cgaggtcctc gaggagcgcc tcgttccccc tgacggtgac gcacgcgtgg
     5281 cggcgcgaga cggtgccgtc gtcgacgaag acggcgcact ccgggtcgcg gccgatcacg
     5341 ttctctcccg ccgcgagcgg gaacgcgcgc ccgtcccgca cgagggagaa cgcgctcgcg
     5401 tgcgcggccc gcacgcccga atccgccgcc tcgccggaga acgcgtagcc gaagccgtgg
     5461 accgtgcgca ggaagccgtc gcgatgcccc ttctccccca tggcctcccg cagctcggcg
     5521 acgaggttcg cgaggttcgc ctccacgacg aacgtgtccg gccacagcgt ctcctggagc
     5581 tcggccttcg ccatggcccg cgggcggttt ctcacgagga tctcgaggag gtggaacgcc
     5641 ttcggcgaca acgggacggg cgctcccccg cggaagagct ggcggctctc cgggtcgaag
     5701 aggcaatcgc cgaacctcag ggccacgtgg atctctccga agaataccaa ttccctgcga
     5761 atgacctgtg aaatccccgg gggcgagccc catgttgtcc ccatgagtgg cgcaccggcc
     5821 gggcggatcc cttcagcggc ggcgatcgcg ctcctcctcg ccggcgcctt ccgggtcgcc
     5881 ggccaggagc ccacgatcct cgccggcgag gcgtttacgc ggaccgcctt cgggatgacg
     5941 ttcgaggtgc ccgcccggga ccgcagcacg gtcgacgaag tcttcgcggg tctcggctgg
     6001 atcccgtccg gcccggacgg caagaccctg aaccccgagg ccgcgttcct cctctggcgg
     6061 cagcgcgacg gcggcgcgcg gcgcctgagg gctgtcctcg tgggcctcta cgacgacgtg
     6121 cgctggaacc gccggatcgc ggagagcacg gacctcgtcc tgacgttcga gaacacgacg
     6181 ttcccgtggg cccggtccga gtacgcgagc ggggagcgcc tcgagagcga agaactgaac
     6241 tggggctcgg tacgggcagg cgccggcctc gcattccgat cgcctctcgc gccgggtcac
     6301 gccgacaact tcctcgaagg gtccctctct tacgaaccgg gctttctcta cttttcgaag
     6361 ggctcgtcga cggcgacgga tttcacgccg ccgtcgaatt cgtacgaggg gcgcttccat
     6421 ctgcgagttc gtgccgacgc cttcacgcgc aacctcctcg agctgcccca ccggggctgg
     6481 gcggccgggc tcgacgcctg ggccgcccac cgcggcggct ggcaggactg gggcggcggt
     6541 ccgacggggg cgcacgccgc ggcgtatacc cgttcgtgga ccgccctgag cggatacgcc
     6601 ctcgcggcgc tcccgctgcc cctcggggac cggaggcaca gaatcctcgc gtccgtgtac
     6661 gccggaacag gatcggacct cgaccggttc tcggcgttcc ggctctcggg cggctcgaac
     6721 gccggggact acgagacgat cacacggccc gtcctcccga cggccggcct cgacgagatc
     6781 gtctcgcgcg gctacgtgat cgccggcctc gaggcccgga tgcagctcgc cttctttctc
     6841 ttcctccacc tcaggggcac gctcgcgtcc gtggaccgga ccgtccggga cgcgtcgggg
     6901 gcgatcctcc agcgcaccga cagcccgaac gccgtcacga tcggcctttc gagcggcttc
     6961 ttctggtcgt cgatgatcga gctttcgtgg tcgcgcaacg cagcgctcga aagccgggag
     7021 ggggacggct tcacgtccgg ccgagacggc gtctacttct cattcaccag gagcttctga
     7081 caaattgtgc gtcccggggg cgcgcgccta gaatcggcac ggtcacgtga cgctccacgc
     7141 aggcgatcgc ctcggtgcgt acgagatcct cgcgccgctc ggctcggggg gaatgggcga
     7201 agtctggcgc gcgcgcgaca cccgcctgaa gcgcgaggtc gcgatcaagg tcctgccgga
     7261 ctcgctggcg tccgaccccg tcgctcgcac gcggttcgac cgcgaggccc gcgccgtggc
     7321 ggctctctcg cacccgaaca ttctcgacat ccacgacgtc ggctccgagg gcgggatcgc
     7381 ctgggccgtg acggagctcc tcgaaggcca gacgctcaag gcgcggctgg acgagggcgc
     7441 actgccctgg aggcgggccg tcgaggtcgc gctcgcgctc gcggagggcc tcgaagcggc
     7501 ccacgcccgc ggcgtcgtcc accgcgacat caagccctcg aacgtgttcc tcacgtccga
     7561 cgggcgcgtg aagatcctcg acttcggaat cgcgacgctc gacgattcga cgagcctctc
     7621 gctgggcgcg cgcgacgtga cggagcctca cgcgatcgtc gggaccgccg ggtacctttc
     7681 ccccgagcag atccggcgcg aggccgccga cgcgcgcagc gacatcttct cgctcggatg
     7741 cgtcctctac gagatggtca cggggcgtcg cacgttcccg ggcgacacgc cccccgaggc
     7801 gctcgtcgcg acgctgcgcg gcgagccgcg cgatccggcc gacctcgtca ccggtctccc
     7861 tcccgagctg cgccgcctga tcctccgatg cctcgagaag cggccggacc gccggttcca
     7921 gacggcggcc gacctggcgt tcgcgctgaa gatccctttc acgggctcgg gcggcgggca
     7981 cgagttcgtg tcggcgacgg acgagaccga ggtcatcgct tcggcggcga acgccagccc
     8041 gaagcggcgg gccgcctcgg tggcggccgg cggagccctt ttcgcctcgg tcctcgccgc
     8101 cgccgcgtgg atgcttttct tccgcggcgc cggacccgcg ggcaacgtcc tcgccgtcct
     8161 ccccttcacg aacgaaaccg gcgactcggc gcacgagtac ctctccgagg gcgtgaccga
     8221 ttcgctgatc gacgcgctct cgaagacccc cgggctctcc gtcctcgcgc ggtcgaccgt
     8281 ctacctggtg ccggaccggc acgacccgcg caaggccggc cgctttctcc atgcgcgcac
     8341 ggtcctcgtg ggccgcgtga agcggcaggg cgccgatctg cacgtcgacg ccgagctcgt
     8401 cgacgtcgcg accggggcgc gcctctggca caagatcgtg acggaccgcg cgggcgatcc
     8461 ggtcccggca gccgcggacc tcgccccggg catcgcgcgc accctcaggc ccgcgctgac
     8521 ggccgaagag gaaaagaacg tcgcgggccg gcgccacgaa aacccggaag cgtgggaccg
     8581 cgtccagcgc gggcggttct tcctgaagag gcgcacggag gaaggaatct tcaaggcgct
     8641 cgggtacttc caggacgcgg ctctcctcga gccgcgcgag gccgacgcgt ggtgcggcat
     8701 ggcggaggtg taccacctcc tcgcgtacta cagctacgcc gagcccgccg aactcgtccc
     8761 gaaggagcgc gaggtcgcgg cgaaggccgc cgccctcgac ccgacgctct cgggcccgca
     8821 cgcgatcctc gcggacatcc ggtacctcac cgaccacgac tggccgggcg cggaggagga
     8881 gttccggaaa gcgatcgccc tcaacccgag cgacgccgag gcgcaccagt ggtactcgaa
     8941 tttcctctcg gcggcgggcc gccccgacga ggcgctcgcc gagatcctgc gcgcccggac
     9001 gctcgatccc ctcagcgcgg taatccaggc ggacgtcggg ctcgcgcgct acttcggcgg
     9061 agacatggcg ggcggcgaga aggagatccg tcgcgcgatc gagctggacc cgcacgcctt
     9121 cctcccgcgg ctctgggcct ccctcccgct ccttgcgcag aaacgctacg acgaggccct
     9181 cgtggaactg ggcaaggcgc gcgagatcga gcgcgccgtg ccggagccga tcgctttctg
     9241 gggctacgcg agcgcgctcg ccggccggcc cgcggacgcc gacgccgccc gccgcgacct
     9301 cgacgccctc aagaaagagc gctacgtggg cgggttcccg tacgcgatcc tgacactcgg
     9361 gcagaagaag cgggaggaat cgctcgactg gctcgagaaa tctgccgcag ccggcgacgg
     9421 ccgcctcgcg tacctcgggg tcgagccggg gttcgacccg ctgcgaagcg atccgcgttt
     9481 caccgcggtc gtccggcacc tcgcgatccc gaagccgcgc tgaatcagcc gaagtccgcg
     9541 agcgccttcg cgaggcgcgg cccgggcttc ggcacggccg cgcggatctc gggcacgagc
     9601 atccgcgcgc gcacgcgtgc agacggctcg cgcagggcgt actcgaggag gtctgccgcc
     9661 gacgcctcgt ccgcggggtt cgagcgcacg gcgcgcgcgc gctcggcgcc ggcggccagg
     9721 gtgcggcccg acgcctcgta tcgctcggcg aggaaacggc gcgcgtcgac gttcgacggg
     9781 gcgttgacga gcgcccaccg cgcctcgtcc catgcctcgg actcgcggcc cagcttcacg
     9841 aggacgcggg cgtgcgcgag ccggacggac gcagcgccgt cgccgttctc cacggcgtcg
     9901 tcccaggcgt cctcggcctc gacgagcttt ccgcgcgcct cgaggaacgc cgcccggtgg
     9961 taggcgatgg ggtacgagtc cggcaggagg cgtgctccct cctcgaactc gcgcccggcc
    10021 tcctcgaggc gcccggccgc ctcgtagacc ttcgcgaggc cccagtgccc gttctcgtac
    10081 gtggagaaaa tctggatcga gcgctggtag cagccgatcg cctcgtccca cttcttctcg
    10141 tcccagcgct gtttcccgag gagcgcccag gcgtcgtaat agcgtggaaa gatctcgatg
    10201 gcgcgcgtga ggtggcggat cgtcgtctcg cggtcgccgc ggagctgcgc ctcccacgcg
    10261 acgttgtagt gggccttcgc cgagttcggg ttcttcgcga ccatgtcgcg gtacagcgtc
    10321 cggtcgtccg cccaggccag attccggacc gccgccgcca cgccgtacgc gagcaccgcc
    10381 gcggcgagga gggcctcgcg ccacggaaag cgcgacggcc gcggcacctc gagggcgagg
    10441 cctgcgacga tgcccacggc gagcgccgcc aggccggcgg acggaaggta cgcgaggcgc
    10501 tcggcccaga tcgtcccgat cacgaacggg acgttcgaga cggggaacag agacccgccg
    10561 aagagagcga tcccgagcgc ggcgagcggc cggcggcgcc agaacgcgag cgcgaggacg
    10621 aggagggcga ccacgaaggc gacgcccgcc cacgcgcgcc actcggccag atccgcggcg
    10681 agcgtcaggg cgtatgccga gtgatccgcc gccaggcgca ccggcgcgaa cgtcttcagc
    10741 gcgtagagcc cggccagggt gagagcgttc gcgacacgga gcgcgggctt catcgcgacg
    10801 agagggttct cgaggtcgaa gatccccgcc tcgcgcgaga cgagaaagcc gtggatcacg
    10861 atcacgcgca cggcgaagag aacgacgatc gggccgagga accccgtcag cgcccaccgc
    10921 gcggaacgcg ggagcgccgc ccacgcctcg cgcggcgagc gcccgcggcc gccgcggaag
    10981 agctcgccga ggaggacgac gcccggcgcc acgatcgcgt tctccttgac gaagacggag
    11041 agcaggaaga cgccgagagt ccccgcgtac ggcccggccc ggagccgcgg gcccgccgtc
    11101 gcccggagcc agaggcgcgc gacggcgaag acggccagcg ccgcgagcag ctcggcccgc
    11161 ccgacgaggg acgtcacggc ctcgacgtgg atcgtgaaca cggcgaacag cgcccccgcg
    11221 gcgatcgcgg cccgtgaggc gaaacccagc tcgacgagcc aggctgcgaa gaacacggtc
    11281 accgccgcgt ggagggcgat gttcaccgcg cggaagagcc acgcgcgatt cccgtggatc
    11341 cagcgctgga cgccgtacgt cagaagaagg accggccggt agttctgggc cgaggcgagc
    11401 gacccgccga agtattgcgt cgtgaagact tcgccgacct tcgacggcga ggcgatgcgg
    11461 tcgttgcccg agatgatgag cttgtcgtcg tacgtgaagt ctgcgggcag tccgttcaag
    11521 aacggcagga tcgcgaggat cgcgaggccg agtgcgccgg cccggccgag gcgggcgggg
    11581 gggccggcgc tcacgcgggg cggc
//
LOCUS       DAOMFY010000109        11521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1750_length_11521_cov_7.485610, whole genome shotgun sequence.
ACCESSION   DAOMFY010000109 DAOMFY010000000
VERSION     DAOMFY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1750_length_11521_cov_7.485610"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2243)
                     /locus_tag="PLB01_10380"
     CDS             complement(<1..2243)
                     /locus_tag="PLB01_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HQQ77751.1"
                     /translation="MEELLSLIQGLPKPLKYLGAVVLFIPLGYFLIKLLGLEKYWWLF
                     LLGLLVVVAAVVLFEFLRKGREKQKGRAFEGELRKDSQAGGASKEEVREALKDLSAKW
                     LEAVQNLKGAGLDIYSLPWYLLIGEPQSGKSTTLKNSGLEFPVGGDALSGAGGTRNCD
                     WWFANEAVILDTAGRFTFQEEAAPDASEWSSFLKLLKKHRGYCPINGVLVVIPATSLL
                     EDTPEEQDKKAQNIRAKLIHIQRLLDIRFPIFFLITKADRALGFSEFFTKLDPVEQRQ
                     LFGWSNPAGPEKPYDPNVVDAAWDQIVTRVHKLRLKFLAAEENVANADRFIVFPEELR
                     ALKDPVRRYLDGILTQSRYDEPFVFRGFYISSGVQQGKPIAQATRDLLKGPSGSAGDV
                     IESLENIFKKSRAFFIKDFYEKKVFPEQGLVARTRAAAEREKKTTLAFRVLTWAILPL
                     IILTMIPAFLSLRRILNPIRTHAAQAQACMKVPCTIAQKYELTRLLYEDRVSLGQHPF
                     LLGLFLRGAKSNEIHTLLATVQQKLFFEGVVSPLLKESEARMASLNWETFSDYPAFFG
                     ALQSFLGWRGFEMSLKKADGTDAFRAEDLRLLNLANFAQKTKGVAGTEYAAQIDEWAA
                     QISPADPRPDAFVTQLARGRAAGELKVGVPDPSRAIDTFDRFWTVANLARWDWKLMKG
                     LEEWVRLYTALTGIVDPQQASYLKRTSDTAKPFKANFDALAKHLETARPGERGFPGAT
                     IEQWTRYL"
     gene            complement(2250..2933)
                     /locus_tag="PLB01_10385"
     CDS             complement(2250..2933)
                     /locus_tag="PLB01_10385"
                     /inference="COORDINATES: protein motif:HMM:NF021377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HQQ77752.1"
                     /translation="MDSTSTTDLFGVSSRLFLFLSSFRQKVRRNVRLEPDAVAADLQE
                     IFDDQARAVRGDPRLDALYEKARYPLVVLADEILLHSGWDRSAEWEQHLFEEKYFKTN
                     IGGDQYFSIAKDLRPDDVELAAIVFTGLALGFRGKYRERPEKAVEVKNRVYRLLAEYL
                     ADVGDKITPEAYHVTAAPARKLSPAVTLARVAIVGVGILFLYYAITWLMWARSVDDLR
                     TVVQAMGLA"
     gene            complement(2926..4296)
                     /gene="tssK"
                     /locus_tag="PLB01_10390"
     CDS             complement(2926..4296)
                     /gene="tssK"
                     /locus_tag="PLB01_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HQQ77753.1"
                     /translation="MRKNRPVLWAEGMFLRPHHLQAAERFREETLHEEVRRIQPFFWG
                     VTRMEVAADQLENHVFELRDIEAKLKDGSILSAGANLRIFPRPFKAELEQAGGRMDVF
                     LGVPVLREDAPNTFFPGEDRRGQDRRLTSEVLEIPDENTGANRQPVETRLVNGRLFFG
                     NENRDGYECLPLAIVERSGQGKNFPVLSKDFIPPVTEIGASATLQTLCESVTNRLEAK
                     HRLVLTEVASGRLAIASEGTSGWQTLMKLKILGSFVYILQQLTRIPRVHPFPVYCEFA
                     RLLGDLAIFDEGLRPLKVPLYDHDRLGPCFFDICHNIELLTEKVVASRFIRVEFVLRE
                     DLLVADLKPEWFTSEAEFYLSVETDLPERDLKERLNTMKVGAVHDIPVLRQRRLFGLD
                     LEMLKRIPSGLPAGDDHYYFTIEKEGIYWGNVVKEHAMAVSGMLDPKLKFFLYVVSKP
                     LSATRG"
     gene            complement(4293..4766)
                     /locus_tag="PLB01_10395"
     CDS             complement(4293..4766)
                     /locus_tag="PLB01_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77754.1"
                     /translation="MPTSAGTPARAFRLASLFLAVACAALAGGCGSTAATVAFRCAPE
                     INGGTLLTVDVVRATEPEVERIRALGEKWFYDPLRESLRDRTRTATFSQAEGRCEKEL
                     TVPASKDDKFLVIVADYRFQSADPGRHLVVLRKDQWKGRTIRVQVRDREFSIVQP"
     gene            complement(4772..6649)
                     /locus_tag="PLB01_10400"
     CDS             complement(4772..6649)
                     /locus_tag="PLB01_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ77755.1"
                     /translation="MSGPVFVAVEVWLGPMEGLRHVTPAPDSRATVHIGRVSVDKKTS
                     TRNEFVLSYGYGVSSAHAEVRFEKGRLFLKDNGSTNGTSVQSGQVSGEVEIHSGDVFL
                     VSSTPVRVRLVDATLPEMPFPRVLRGEAYPDPAVQSIVRGAREAATQRGELFADSRHL
                     CDALLRSRSATIQRILRDSNWSREQALAELWEGRLFAGSYEWLNDLFLQPLGLKTGSD
                     ELILSPKVGFLLEECQRRTIESSAMDLETTLRHELLKLLRQDEESAVGSWLARRLPDF
                     APREEPSRPAPAPAPPARPAAAPPPAPEPVHKAAPARRSETPRRESAPEPAVPGAAIA
                     AAPLTHEEIALDRKARDLADDLLREAAAYRFSTSADRREALKSLVLKELHTLAPAKRP
                     RVLEQLRLQFPVAVAGDVPNLEEARLRRRVAELERRLAEAASQPAAPAPKTAAPAALP
                     WKALLAPEDGKAPALEPRLAVLRDVLRFAQDVETFTLGLVEAMTMPGAETASFRLPNF
                     RDRLSGFLAAAAEGKDVNLDDVRDYLGELRRWQIAISAGHHEAPKLWFTRLWKRISPS
                     VIEAGKAGAGWKLRGETAEWWNQYRDLVKDLNPDLVQDQVLQAAAHLSQTEFQKLNKR
                     K"
     gene            complement(6646..8778)
                     /locus_tag="PLB01_10405"
     CDS             complement(6646..8778)
                     /locus_tag="PLB01_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77756.1"
                     /translation="MQEPGGFGTARLLPSAGANAAFALHLVLDAPGRDHARSPWVRLS
                     EADDFARVALAAVMGDPGTLLELCALKLMPDVWPEGATPAALGLTNRDLEDRWERESA
                     RLVELDAVSPHFPRPVLLEGSAEAPRRLPPLVFSHPARSFFVPPCPRCRAPLSLCRDE
                     EWLANAGLPPYATTLDRFLWCESCARGPEAPTVYTTSRVSAPRVAVRSAPDLLDDLGR
                     ALREVWTEDEAIALVGSETARAVRAFPAASGASVRDLFTFLTFFGSPLLLTVAAPFDL
                     EALADALGGRPWEELAAPGPAPDLAFPPRWAWLVRPPAPTRRLLFSSESAGLDAVEIF
                     ALKMSAFRQVLDGLIDYYRATGRPHLDLHPRHLLFDLSGAGDGLPVFWTFQVRIHGLA
                     AATMPLPAPGSPTVVLPGHGLAVPYAAPEVQEFRLAGFRPGEFVFTDIDEEGTAARRF
                     RLRGRISDPYGLYPSPRPRDAIFVSLPDPTLAFGFTGLAVRATPGETRSDEAAFASDL
                     LELEESGLRRLRLALGTKLPGARYRVYPDFGTPTDLYALGVALLRVLLRNDEQDLGAV
                     LKAADRLGRELAAVAPEARRDVRLAALLKRAPDAAPVFAKTQIFWGRDDRRPSRPNAI
                     PDGLWERALLLALRLVTRVPGFAVTSGPADFDPLFREEKLERVLPEVVEILAELQSLL
                     FDRQPIHIEIQQILAEIREDERSSGGRA"
     gene            complement(8832..9902)
                     /locus_tag="PLB01_10410"
     CDS             complement(8832..9902)
                     /locus_tag="PLB01_10410"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ77757.1"
                     /translation="MRTDLTQIDLSPIGELVRIRKEETVLEERLAKMEAKNPGVSPVV
                     YARVRSDYEARRNELARGSQPLKERALREYQRLKVLRAEVESAVQQVSLEKEELEFRR
                     DLGEFPDDEFTKRIADCDKRLAEERQQLEGVLQMRNTFLAAFRSEEELEAGSPQPGAP
                     APAIPRPPQPAPPQPAPAQPAPPQPAPLQPPPPPPAPRAAPSAPPASPVAYAPAGDGL
                     TPDGTVITAMRPPVGGARIPSSHEIPAEDIPVAPPSHSATAVLPRARLTLLEGEKPVK
                     EFLLKPGVSVIGRLAQSDIQLPSPDVSRRHAQVTAGPDGCFVADLGSENGVKVNGRHV
                     ESQRLEDGDIVELGKQRLVFRE"
     gene            complement(9987..11306)
                     /gene="pepQ"
                     /locus_tag="PLB01_10415"
     CDS             complement(9987..11306)
                     /gene="pepQ"
                     /locus_tag="PLB01_10415"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HQQ77758.1"
                     /translation="MADLYAQHLVRLAASTARALEETGFDRMVVHSGVPFTYFADDND
                     APFRSTPTFAHWAPVEGPYHLLDVVPGKRPRLVRVQPRDYWYAPPAPAPAFVTREFDV
                     VDVATSEAAWKALGNGFARTAFVGGAVDESAAHGFRPENANPKGLVARLDWERSYKSD
                     WEAEQVAVANAKAARGYRALEKAFRADASEMELHHAYLTAIGDVEEALPYTSIVALDD
                     RSATLHYHGKRGRLPSSSKVLLVDAGATVNGYASDITRTWVKEGVEPVFARLLDGMKA
                     LQAKLCLEGRPGLHYRDFHLRAQRYVGELLREVGVLKISAEEAVTTRLTFPFFPHGLG
                     HFLGLQVHDVAGRQADRTGTPAPPPADHPALRTVRPIEERMLFTVEPGLYFIPMLLEP
                     VREGPHRAAIDWPLVDRLIPMGGIRVEDNIFVGKDANRNLTRESLPE"
     gene            complement(11315..>11521)
                     /locus_tag="PLB01_10420"
     CDS             complement(11315..>11521)
                     /locus_tag="PLB01_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77759.1"
                     /translation="ELEAARLGASARLETLQEDYAAARARAGAAAEAKLEELRTEIER
                     TRRELHETLQRLVEQAEGLGLAPA"
BASE COUNT         1730 a         3795 c         4232 g         1764 t
ORIGIN      
        1 aggtagcgcg tccactgctc gatcgtcgcg ccggggaagc ccctctcgcc gggacgcgcc
       61 gtctcgaggt gcttggcgag cgcgtcgaag ttcgccttga acggcttcgc cgtgtccgag
      121 gtgcgcttga ggtagctcgc ctgctgcgga tcgacgattc ccgtgagcgc cgtgtagagc
      181 cggacccact cttccagccc tttcatgagc ttccagtccc agcgcgcgag gttcgcgacg
      241 gtccagaacc ggtcgaacgt gtcgatcgcg cgggacgggt cggggacccc gaccttcagc
      301 tctcccgcgg cgcgcccgcg ggcgagctgc gtgacgaacg catccggacg cgggtcggcc
      361 ggcgagatct gcgccgccca ttcgtcgatc tgcgcggcgt actcggtccc ggccactccc
      421 ttcgtcttct gcgcgaagtt cgcgaggttc aggaggcgga gatcctcggc gcgaaaggcg
      481 tccgtgccgt ccgccttctt gagggacatc tcgaaaccgc gccagccgag gaagctctgg
      541 agcgcgccga agaacgcggg atagtcggag aacgtctccc agttcagcga cgccatccgc
      601 gcttcggact ccttgaggag cgggctcacg acgccctcga agaacagctt ctgctggacg
      661 gtcgcgagga gagtgtggat ctcgttcgac ttcgcgcctc tgaggaagag gccgaggagg
      721 aaggggtgct ggccgaggct gacgcggtcc tcgtagagca gccgggtgag ctcgtacttc
      781 tgggcgatcg tgcagggcac cttcatgcac gcctgcgcct gcgcggcgtg cgtgcggatc
      841 gggttcagga tccggcggag cgagaggaac gccgggatca tcgtgaggat gatcagcggc
      901 aggatcgccc acgtgaggac ccggaacgcg agcgtggtct tcttctcgcg ctcggcggcc
      961 gcgcgcgtcc gcgcaacgag gccctgctcc gggaagacct tcttctcgta gaaatccttg
     1021 atgaagaacg cgcgtgattt cttgaagatg ttctcgaggc tctcgatgac gtcgcccgcc
     1081 gagcccgagg ggcctttcag gaggtcgcgc gtcgcctggg cgatcggctt gccctgctgc
     1141 acgccgctcg agatgtagaa cccgcggaag acgaacggct cgtcgtagcg gctctgcgtc
     1201 aggatgccgt cgaggtagcg ccgaaccggg tccttgaggg cgcgcagctc ctcggggaac
     1261 acgatgaacc ggtcggcgtt cgcgacgttc tcttcggcgg cgaggaactt cagcctgagc
     1321 ttgtgcacgc gcgtgacgat ctggtcccac gccgcgtcca cgacgttcgg gtcgtagggc
     1381 ttctcggggc ctgcgggatt cgaccagccg aagagctgcc gctgctcgac cgggtcgagc
     1441 ttcgtgaaga actccgagaa cccgagcgcg cggtcggcct tcgtgatcag gaagaagatc
     1501 gggaaccgga tgtccaggag gcgctggatg tggatcagct tcgcccggat gttctgcgcc
     1561 ttcttgtcct gctcctccgg ggtgtcctcg aggagcgacg tcgccgggat gacgacgagg
     1621 acgccgttga tggggcaata gccccggtgc ttcttcagga gcttgaggaa gctcgaccac
     1681 tcggacgcgt cgggagcggc ctcctcctgg aacgtgaagc gccccgccgt gtcgaggatg
     1741 acggcctcgt tcgcgaacca ccagtcgcag ttgcgcgtgc cgccggctcc cgagagcgcg
     1801 tctcccccca ccgggaactc gagccccgag ttcttgaggg tcgtgctctt gcccgactgc
     1861 ggctccccga tgaggaggta ccagggcagc gagtagatgt cgagccccgc acccttcagg
     1921 ttctgtaccg cctccagcca cttggcggag aggtccttca gggcctcgcg cacctcttcc
     1981 ttcgaggcgc cgccggcctg cgagtccttg cggagttcgc cctcgaaggc ccggcccttc
     2041 tgcttctcgc ggcccttgcg caggaactcg aagaggacga cggccgccac gacgacgagc
     2101 aggccgagga ggaagagcca ccagtacttc tcgagcccga ggagcttgat gaggaaatag
     2161 ccgaggggga tgaagagaac gaccgcgccg aggtacttga gcggcttggg cagaccctgg
     2221 atgagagaga ggagttcttc catggccgcc tacgccagcc ccatcgcctg cacgacggtc
     2281 ctcaggtcgt cgaccgagcg tgcccacatg agccacgtga tcgcgtagta gaggaagagg
     2341 atgcccacgc cgacgatcgc gacgcgtgcg agcgtgaccg caggggagag cttgcgcgcc
     2401 ggcgccgccg tcacgtggta ggcctcgggc gtgatcttgt cgcccacgtc cgcgaggtac
     2461 tcggcgagga gacggtagac gcggttcttg acctcgacgg ccttctccgg gcgctcgcgg
     2521 tacttgccgc ggaagccgag cgccagcccc gtgaagacga tcgccgcgag ctccacgtcg
     2581 tcgggacgga ggtccttggc gatcgagaag tactggtcgc cgccgatgtt cgtcttgaag
     2641 tacttctcct cgaagaggtg ctgttcccac tccgcgctgc gatcccagcc ggagtgcagg
     2701 aggatctcgt ctgccaggac gacgagcggg taccgtgcct tctcgtagag cgcgtcgagg
     2761 cgcgggtcgc cccggacggc gcgcgcctgg tcgtcgaaga tctcttggag gtcggcggcg
     2821 acggcgtcgg gctcgagccg cacgttgcgg cggaccttct gccggaagga gctgaggaag
     2881 aggaagaggc gggagctgac cccgaagagg tcggtcgtgc tcgtgctatc cacgggtcgc
     2941 gctcagcggc ttcgagacga cgtacaggaa gaacttgagc ttcgggtcga gcattcccga
     3001 gaccgccatc gcgtgctcct tcacgacatt gccccagtag atgccttcct tttcgatcgt
     3061 gaagtagtag tgatcgtctc cggccggcag cccggacgga atgcgcttga gcatctcgag
     3121 gtcgaggccg aagaggcgcc gctgccgcag cacgggaatg tcgtgcacgg cgccgacctt
     3181 catcgtgttg agccgctcct tcaggtcgcg ctcggggaga tccgtctcca ccgagaggta
     3241 gaactcggcc tcgctcgtga accactcggg cttcaggtcc gcgacgagga ggtcctccct
     3301 caggacgaac tcgacccgga tgaagcgcga cgcgacgacc ttctcggtca ggagctcgat
     3361 gttgtggcag atgtcgaaga agcaggggcc gagacggtcg tggtcgtaga gcggcacctt
     3421 gaggggccga agcccctcgt cgaagatggc gaggtcgccg aggagacgcg cgaactcgca
     3481 gtagaccggg aacggatgca cgcggggaat ccgcgtgagc tgctgcagga tgtagacgaa
     3541 gctgccgagg atcttcagct tcatgagcgt ctgccagccc gaggtgcctt ccgacgcgat
     3601 cgcgaggcgc cccgaggcca cctcggtcag gacgaggcga tgcttggctt cgagccggtt
     3661 cgtgaccgac tcgcagagcg tctggagcgt cgccgaggcc ccgatctccg tgacgggcgg
     3721 aatgaagtcc ttcgagagga cggggaagtt cttcccctgg cccgagcgct cgacgatcgc
     3781 gaggggcagg cactcgtagc cgtcccggtt ctcgttcccg aagaagagcc gtccgttcac
     3841 gagacgcgtc tcgacgggct ggcggttcgc cccggtgttc tcgtccggga tctcgaggac
     3901 ttcggacgtc agccgccggt cctggccccg ccggtcctcg cccgggaaga acgtgttcgg
     3961 ggcgtcctcg cggaggacgg gcacgccgag gaacacgtcc atccgccctc cggcctgctc
     4021 gagctcggcc ttgaaggggc gcgggaagat ccgcaggttg gcgcccgccg acaggatcga
     4081 tccgtccttc agcttcgcct cgatgtcgcg cagctcgaag acgtggttct cgagctggtc
     4141 cgcggcgacc tccatccgcg tgacgcccca gaagaaaggc tggatgcgcc gcacttcctc
     4201 gtggagcgtc tcctcgcgga atcgctccgc cgcctgcagg tggtgcggcc gaaggaacat
     4261 gccttcggcc cagaggaccg gacggttctt cctcacggct ggacgatcga gaattcccgg
     4321 tcgcggacct ggacccggat cgtccggccc ttccactgat ccttgcgaag gacgacgaga
     4381 tggcggcccg ggtcggcgct ctggaagcgg tagtccgcga cgatgacgag gaacttgtcg
     4441 tccttgctcg ccgggacggt cagctccttc tcgcagcgcc cctcggcctg cgaaaacgtg
     4501 gcggtccgcg tccgatcccg cagggactcg cggagcgggt cgtagaacca cttctcgccg
     4561 agggcgcgga ttctctcgac ctcgggttcg gtggcgcgga cgacgtcgac ggtgagaagc
     4621 gtcccgccgt tgatctcggg cgcgcaacgg aaggcgaccg tggccgccgt cgatccgcag
     4681 ccgccggcga gcgcggcgca ggcgacggcg aggaagagcg aggcgagtcg gaaggcccgt
     4741 gcgggcgtgc cggcggaggt cggcatggcg tttatttcct cttgttgagc ttctggaatt
     4801 ccgtctgcga gaggtgcgcc gccgcctgga gaacctgatc ctggacgaga tcggggttca
     4861 agtccttcac gaggtcccgg tactggttcc accattccgc cgtctccccg cggagcttcc
     4921 atccggctcc cgcttttccc gcctcgatga ccgaggggct gattctcttc cagagcctgg
     4981 tgaaccacaa cttcggcgcc tcgtggtggc ccgccgagat ggcgatctgc cagcgccgga
     5041 gctccccgag atagtcccgg acgtcgtcca ggttcacgtc ctttccctcg gcggctgcgg
     5101 ccaggaagcc gctcagccgg tcgcggaagt tgggcaggcg gaaactcgcg gtctcggcgc
     5161 ccggcatcgt catggcctcg acgaggccga gggtgaacgt ctccacgtcc tgggcgaagc
     5221 gcaggacgtc gcgcagcacg gcgaggcggg gctcgagggc cggagccttg ccgtcttcgg
     5281 gcgccagcag cgccttccac ggcagagccg cgggggcggc cgtcttcggg gcgggagcgg
     5341 ccggctggga ggccgcctcg gcgaggcgcc gctccagttc cgccacgcgg cggcggagtc
     5401 tcgcctcctc gaggttcgga acgtcgccgg ccacggcgac gggaaactgc agccggagct
     5461 gctcgaggac gcgtggcctc ttggccggtg cgagagtgtg aagctccttg aggacgagcg
     5521 acttgagcgc ctcgcgccgg tcggccgacg tcgagaagcg atatgcggcg gcctcgcgaa
     5581 gcaggtcgtc ggccaggtcg cgcgccttcc ggtccagggc aatctcctcg tgcgtgagcg
     5641 gcgcggccgc gatcgccgct ccgggaacgg cgggctccgg tgcgctctcg cgtcggggcg
     5701 tttccgatcg gcgtgcgggg gccgccttgt ggacgggctc cggggccgga ggaggggcgg
     5761 cggcgggccg cgcgggagga gcgggcgccg gagccggacg ggacggctct tcccggggcg
     5821 cgaaatccgg aagccgccgg gcgagccacg agccgacggc gctctcctcg tcctgccgca
     5881 ggagcttgag cagctcgtgc cgcagcgtcg tctcgaggtc catcgccgag gactcgatgg
     5941 tccggcgctg acactcctcg agaaggaagc cgaccttcgg cgacaggatc agctcgtcgg
     6001 agccggtctt gaggccgagc ggctggagga agaggtcgtt cagccactcg taggatcccg
     6061 cgaagagacg accttcccag agctcggcca gcgcctgctc gcgggaccag ttcgagtccc
     6121 tcaggatccg ctggatggtc gcgctgcgcg aacggaggag cgcgtcgcag aggtggcggc
     6181 tgtccgcgaa gagctcgccg cgctgcgtcg cggcctcgcg ggctccgcgc acgatgctct
     6241 ggaccgcggg gtccgggtac gcctcgcccc tcagaacgcg cgggaacggc atctccggga
     6301 gcgtcgcgtc gacgagccgg acccggaccg gcgtcgacga cacgaggaag acgtcgcccg
     6361 agtggatctc gacctccccg ctgacctgcc cggactggac ggaggttccg ttcgtgcttc
     6421 cgttgtcctt gaggaagagc cgcccctttt cgaagcgaac ctctgcgtgg gccgacgaaa
     6481 cgccgtagcc gtacgagagg acgaactcgt tccgcgtgct cgtcttcttg tccaccgaca
     6541 cgcgtccgat gtggaccgtg gcgcgtgagt ccggcgcggg cgtcacgtgg cgcagtccct
     6601 ccatcggccc gagccagacc tcgacggcga cgaagaccgg gccgctcacg cccgccctcc
     6661 ggacgagcgc tcgtcctcgc ggatctcggc caggatctgc tggatctcga tgtggatcgg
     6721 ctgccggtcg aagaggagcg actgcagctc ggcgaggatc tcgacgacct cgggcaggac
     6781 gcgctcgagc ttctcttccc ggaagagcgg atcgaaatcc gcggggccgg acgtgaccgc
     6841 gaagcccggc acgcgggtga cgaggcggag cgcgaggaga agcgcccgct cccagagtcc
     6901 gtccggaatg gcgttcggcc ggctcggccg tcggtcatcc cgtccccaga aaatctgcgt
     6961 cttcgcgaag acgggcgccg cgtccggggc gcgcttgaga agggcggcga gcctcacgtc
     7021 ccgccgggcc tccggcgcca ccgccgccag ctctctcccg aggcggtcgg ccgccttgag
     7081 cacggcgccg agatcctgct cgtcgttccg gagcagtact cgaaggagag cgacgccgag
     7141 cgcgtagaga tcggtgggcg tgccgaaatc cgggtagacc cggtagcggg cgccgggaag
     7201 cttcgtgccg agagcgaggc ggagacgccg aaggccgctc tcctcgagct cgagcaggtc
     7261 actcgcgaac gccgcttcgt cggagcgcgt ctctccgggc gtcgcgcgga cggcgaggcc
     7321 cgtgaagccg aaggcgagag tcggatcggg cagcgacacg aagatcgcgt cgcgaggccg
     7381 gggcgaagga tagaggccgt aggggtcgga gatgcggccg cggagccgga agcggcgcgc
     7441 ggcggtgcct tcctcgtcga tgtcggtgaa gacgaactct cccggccgga aaccggcgag
     7501 gcggaactcc tgcacctcgg gggccgcgta cgggacggcg aggccgtgtc cggggaggac
     7561 gaccgtcggt gaacccggcg ccgggagcgg catcgtcgcg gcggcgagtc cgtgaatccg
     7621 gacctgaaac gtccagaaga cgggaagtcc gtcgccggct cccgagaggt cgaacagcag
     7681 gtggcgcgga tggaggtcga gatgcggccg gcccgtggcc cggtaatagt cgatgagccc
     7741 gtcgaggacc tgccggaacg cgctcatctt cagggcgaag atctcgaccg cgtcgagtcc
     7801 cgcgctctcg gaagaaaaga gcagccgccg cgtcggtgcc ggcgggcgga cgagccatgc
     7861 ccatcgcggg gggaaggcga ggtccggcgc cgggcccggc gccgcgagct cctcccacgg
     7921 cctgccgccg agagcgtccg cgagcgcttc gaggtcgaac ggcgccgcga cggtcaggag
     7981 gagcggcgat ccgaagaagg tcagaaacgt gaagaggtcc cggacgcttg cgccgctcgc
     8041 cgccggaaac gcgcgcaccg cccgggcggt ttccgacccg acgagcgcga tcgcctcgtc
     8101 ctccgtccag acctcccgga gcgcgcggcc caggtcgtcg agaaggtcgg gtgcggaccg
     8161 gacggcgacg cgcggagcgc tgacccggga cgtcgtgtag accgtgggcg cctcgggccc
     8221 gcgggcgcac gactcgcacc agagaaagcg gtccagggtc gtcgcgtagg gcggcagccc
     8281 ggcgttcgcg agccattcct cgtcgcggca gagcgagagc ggagcccggc agcgcgggca
     8341 gggcggaacg aagaacgagc gcgccgggtg cgagaagacg agagggggga gccggcgcgg
     8401 ggcctccgcg gagccctcga ggagcacggg gcgggggaag tggggcgaca ccgcgtccag
     8461 ctcgacgagg cgcgcactct ccctctccca ccggtcctcg aggtcgcggt tcgtgaggcc
     8521 gagggccgcc ggtgtcgcgc cttcgggcca gacgtccggc atcagcttca gcgcgcagag
     8581 ctcgaggagg gtaccggggt cccccatgac cgccgccaga gccacgcgcg cgaaatcgtc
     8641 ggcctcggac agccggaccc acgggctccg tgcgtggtcg cggcccgggg cgtcgagaac
     8701 gaggtgcaga gcgaaggcgg cgttcgcccc ggccgacggc agaagtcgcg cggtcccgaa
     8761 cccaccaggc tcttgcactg gccgtgatga tcggattcca ccgcggcccc gtcaagaagg
     8821 gcggctgttc gtcactcccg gaagacgaga cgttgcttgc cgagttcgac gatgtcgccg
     8881 tcctcgagac gctggctctc gacgtgacgg ccgttcacct tcacgccgtt ctcggagccc
     8941 agatcggcca cgaagcaccc gtcgggtccc gcggtgacct gcgcgtgccg gcgcgacacg
     9001 tcgggggaag gcagctggat gtcgctctgc gcgagacgcc cgatcaccga aacgccgggc
     9061 ttcaggagga attccttcac gggcttttcg ccttcgagga gcgtgagccg ggcgcgtggc
     9121 aggacggccg tcgcgctgtg agacggcgga gccaccggga tgtcctcggc agggatctcg
     9181 tggctcgagg ggatcctcgc gccgccgacg ggcggacgca tggccgtgat cacggtcccg
     9241 tcgggcgtga ggccgtctcc cgccggggcg tacgcgaccg gagatgcggg cggcgcgctc
     9301 ggcgcagccc ggggcgcagg cgggggaggc ggaggctgaa gaggagcggg ctgcggaggc
     9361 gcgggctgag caggtgcggg ctgcggaggc gcgggctgcg gaggccgggg aatggcgggc
     9421 gcgggagcgc cgggctgagg tgatccggcc tcgagctctt cctccgagcg gaatgcagcc
     9481 aggaacgtgt tgcgcatctg gagcacgccc tcgagctgct ggcgctcctc ggcgaggcgt
     9541 ttgtcgcaat cggcgatgcg tttcgtgaac tcgtcgtccg gaaactctcc gaggtccctg
     9601 cggaactcca gctcttcctt ttcgagcgag acttgctgca cggcgctttc gacctcggca
     9661 cgcaggacct tgagccgctg gtactcgcgc agggctctct ccttcagggg ctgcgatccg
     9721 cgtgcgagct cgttccgacg cgcctcgtag tcgctcctca cgcgcgcgta cacgaccggc
     9781 gaaacgccgg gattcttcgc ctccattttc gcgaggcgct cctcgagaac cgtctcctcc
     9841 ttgcggattc ggacgagctc gccgatcggg gacagatcga tctgtgtgag gtccgttcgc
     9901 atcggggcgt ctccggaacc cggagtctag cgctcccgcg ccgcagcggc cgcggagacg
     9961 agcggccccg ccgtccgcac gcggacttac tcggggagcg attcgcgcgt gaggttccgg
    10021 ttcgcgtcct tcccgacgaa gatgttgtcc tcgacgcgga tgcctcccat ggggatcagg
    10081 cggtccacga gcggccagtc gatcgcggcg cggtgcgggc cctcgcggac gggctcgagg
    10141 agcatcggga tgaagtagag gcccggctcg accgtgaaga gcatccgctc ctcgatcggc
    10201 cggacggtgc gaagcgccgg gtggtcggcc ggcggcggcg cgggcgtccc cgtgcggtcg
    10261 gcctggcggc ccgcgacgtc gtggacctgg agcccgagga agtgcccgag gccgtgcggg
    10321 aagaacggga acgtcaggcg cgtggtgacg gcctcctcgg cggagatctt caggacgccg
    10381 acctcccgga gcagctcgcc gacgtatcgc tgggcccgga ggtggaagtc gcggtagtgg
    10441 aggcccggcc gcccctcgag gcagagcttc gcctgcagcg ccttcatccc gtccaggagg
    10501 cgggcgaaaa cgggctcgac gccttccttc acccacgtcc gcgtgatgtc ggacgcgtag
    10561 ccgttcacgg tggcgcccgc gtcgacgagc aggaccttcg aggaggacgg cagcctgccg
    10621 cgcttgccgt ggtagtggag cgtcgcggag cggtcgtcga gcgcgacgat cgacgtgtac
    10681 gggagggctt cctccacgtc gccgatcgcc gtgaggtacg cgtggtggag ttccatctcg
    10741 gaggcgtccg cgcggaacgc cttctcgagg gcgcggtagc cgcgcgcggc ctttgcgttc
    10801 gcgacggcga cctgctcggc ctcccagtcg gacttgtacg agcgctccca gtccagccgc
    10861 gcgacgagcc ccttcgggtt cgcgttctcc ggccggaaac cgtgcgcggc cgactcgtcc
    10921 acggctccgc cgacgaaggc ggtgcgtgcg aagccgttcc cgagggcttt ccacgcggct
    10981 tccgaggtcg cgacgtccac gacgtcgaat tcccgggtca cgaacgccgg cgccggcgcg
    11041 ggcggcgcgt accagtagtc gcgcggctgg acgcgcacga ggcgcggcct ctttccgggg
    11101 acgacgtcga ggaggtggta cgggccctcc acgggcgccc agtgtgcgaa cgtgggcgtg
    11161 gagcggaacg gcgcgtcgtt gtcgtcggcg aaataggtga acggaacgcc cgaatggacg
    11221 accatccggt cgaagccggt ctcctcgagc gcccgtgcgg tcgaggcggc gagacgaacg
    11281 agatgctggg cgtagaggtc ggccatcggt cttctcaggc gggcgcgagg cccagccctt
    11341 ccgcctgctc gacgagccgc tggagcgtct cgtgcagctc gcgccgggtg cgctcgatct
    11401 cggttcggag ctcctcgagc ttcgcctcgg cggcggcgcc ggcgcgggcg cgggcggcgg
    11461 cgtagtcctc ctggagcgtc tcgaggcgcg ccgaggcccc gaggcgcgcg gcctcgagtt
    11521 c
//
LOCUS       DAOMFY010000110        11513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1751_length_11513_cov_6.830599, whole genome shotgun sequence.
ACCESSION   DAOMFY010000110 DAOMFY010000000
VERSION     DAOMFY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_11513_cov_6.830599"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLB01_10425"
     CDS             complement(<1..791)
                     /locus_tag="PLB01_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ77760.1"
                     /translation="MYFKQFYLGCLAHASYLIGDGGEAAVVDPRRDVDEYLDEARAQG
                     LAIKWILETHLHADFVSGHRELAARTGATIVFGDKARAAFPHRGVRDGEEIRLGGILL
                     RFLETPGHTPESVSILVLDPEPRLVFTGDTLFIGDVGRPDLAASPGGVTPQDMAGALY
                     DSLHGKLLTLPDDVAVYPAHGAGSLCGRNISSETSSTIGVQRRTNYALRPMPREDFVR
                     LMTADLPEVPAYFPRDVELNRAGAPPLETRAPAALPAAEVHRLAEA"
     gene            895..2223
                     /locus_tag="PLB01_10430"
     CDS             895..2223
                     /locus_tag="PLB01_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQQ77761.1"
                     /translation="MSTVSEAKNSPVSSADVRAKHKEFLFPSVTNYYKESVVLESGKG
                     MYLKDADGKRYLDFFGGILTVSVGHANDKVNAAVKAQVDRLGHVSTLYPTLPIVQLAE
                     TLARITPGRLQKSTFTGSGTEADETAVVLAQVYTGRLEIIALRHGYSGRSILAQSLTA
                     HATWRAVPTQIAGIKHAPSPYCYRCPLKLTYPSCGVACAKDLEELIKTTTTGQIAGFL
                     AEPIQGVGGFVTPPKEYFEIAVGIVRKFGGVFICDEVQTGFGRTGTHWYGISHWNVEP
                     EIMTMAKGVANGMPLGVTIATPEIADSFKALTISTFGGNPISSTAANATIAVMEEIDA
                     PKRAAEMGARLRAGLEGLKKKYPKTIGDVRGLGLMQGLELVADETVQDRTPAPEKAAR
                     LFEETKARGLLIGKGGLYGNVIRIAPPLIVSASEVDDALRTLDESLAGWA"
     gene            2283..7937
                     /locus_tag="PLB01_10435"
     CDS             2283..7937
                     /locus_tag="PLB01_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ77762.1"
                     /translation="MIGTKLADRYEILSELGRGGMGVVYRAKDPVLNRDVAVKLIPPG
                     NLTKDAEERFQREAQIVAQMDHPSIVPIYDLGRHEGSLFFVMPVLANTNLRQLLREGS
                     LRLGDVLDVGTQVADALDYSHTRGIVHRDIKPENIMTSRDDTGHIRARVMDFGLALAT
                     SEDRLTKTGTLVGTVVYFSPEQVTSRSFDGRSDVYALGTVLYECLAGEPPFTGEVQSV
                     LYRVVHELPQPMRSVGADVSEELEAAVLQCLAKDPEKRPKRAGHLAEALRRYRSKLRE
                     EDFGKSVVFTASRMLPRPAVAAPFIGREKEMAELQRRLHAALGGECEFVVLAGEPGIG
                     KSRLVEELKALARARKIRVLSGRFVEHDRAFAHQGFCELIQDYFRSKDTGLSESSRPD
                     FTDLAGDLLALFPVLSEIGELRAAASSDSGAVKPGDGRKAEDKIAIFELIARTLTRIA
                     GGKPLLLALEELHGAQLSIEALQYVARRLGPTPTLIVGTYRQTEVDKRHPLVKMLESF
                     RGDPRFAALTLGPLTASEHRALVELSAGGGKLTDDLATRLLAATEGNPLFTKELVRSL
                     LDSGGIARDDSGALNLSGATGISSDALPETIQQAVEARIERLPEEVRDVLSVASVLGK
                     SFDFKDLETLAEDAKGLDDAVDRLVKDGLLEEERESRGDRLAFASGIVRDVLYGALSR
                     RKRRTLHRSYADLLEKRHQGRLDRVYPELVHHFSEGDVPEKAVDYSLKLAKKSLDAFS
                     PEEAVRVAKTALDYLSDEEWEGDKSLEGDARLLLAQASRMTGHADAALREAEAAGKIF
                     EREKQPVRAVEAVLFAADTAWNGRKVDEARRLVERGIEAAAAAQDTTHLPKLLSLAAT
                     VANLRGDYARAAAYGSQLERLGGESKKAAEVIASGGRLVVAMTSPVAAVEPAAAHIVE
                     ETEVLGTVFETLLATDADGRIVPALCEEWSLTDHGKSALLRLRHGVRFSDGSPLTATA
                     VKTSLERAVRVRDKGMPAALAAVRGAAEHRKGEAAGVSGIRVVSEDKLEFDFVEPLPI
                     FPALLSDHVAAVARVVPGENGAETAVGTGPFVIAQRTADRIVLERNPHYWKDSALLDG
                     IEFRTGMKASAIVSGLRTGEIELARDLSPQDLDAILREPRLRAGLVETPKKGTYFALF
                     NSKSKLGGNVALRRALAGVTRSQDFVWAALGRFAVPATGILPPGILGHDAGRRRPLMA
                     RDEALALLEGAGLMPPITLTAAVHPILRDRHKALTAALFAIWEELGIEVRVTTTDMKS
                     YLEAQAAATVDLLISRWMADYEDPDDFTFNLFRSGVGYFRSFFSSDETDRLAEEARIE
                     SRPAIREGLYRKLEGLLLDSPVVIPLFHEVDYRIAGPGVRGVALRSSPPFVNYPEIGK
                     TEAPAAHAAPEWGGGVVHVPIGGVVTEFDPATMAILEQAETMPLVFETLTRDLEGGRV
                     VPWLASEVIPEEGGARFRFRLRRGVRFHDGRAVSARDVRYTFERILQKGTSAGKFLLA
                     PIRGAQGLIDGKASDLEGFHVVSPSEFVIELEKPLAFFPVLVSWPGAGIVPESTTAIG
                     ESWRKGCIGTGPYRVASFEPGKRLELERNPAYWREGFPKNDGVVFRFGVPPEEIRSEF
                     LAGRFSIASDLLPADAEALRQDPRFRATYRESPSLVTYFVTFNIHKGVFADARARRAV
                     AQGIDAAAIVRRTLGRHAIPANGLIPPGLLGYVTKPRSHPQASGQVSGAHTVSRETTE
                     FTAVVNPIMFGEFGGFTKELTQAFKEMGYSIRVVNKTMAEFLDAQHNAPTDVMIGRWI
                     ADYPDADTFAQGVLHSREGVNGRYCGMPAIDALTERARGEIDPRTRESIYREVEDIVA
                     REALMIPLFHEQVYRFARPEVEGLSVGFGQPIVLYENLSIKS"
     gene            complement(7947..9089)
                     /locus_tag="PLB01_10440"
     CDS             complement(7947..9089)
                     /locus_tag="PLB01_10440"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HQQ77763.1"
                     /translation="MAADLSVVFTGIRFENPFLLASAPPTESDVNILRAFEAGWGGVV
                     TKTIGLHPVVNVAGPKTKFLRTDTQSPRISMKPRPDSVLHSSWNWELISDKPLDWWVG
                     RLSRIKRAHPGKVLVASIMAGSGNDKELGNWQLLAKTCQDEGVDALELNFSCPHMDRE
                     DMGSNIGKDSVLTSTVTKAVKEVAKVPVWAKLTPSTSDIAVEAGAAFRGGADAISSSN
                     TFPSIPLVDPETLDFEMHVDGYTSSGGLGGPAILPLALAKMAQLTQSFPDRSFSGIGG
                     VSDFSHALNYFLLGCGTVQVCTAAMLDHAIGPNVIRALKDGMAAFLEKNAARGWTSLE
                     DFRGLRRDRVIPQSQIRRPNPGEYHGGYEAEGYAASEPTPANDRVR"
     gene            9141..10658
                     /locus_tag="PLB01_10445"
     CDS             9141..10658
                     /locus_tag="PLB01_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HQQ77764.1"
                     /translation="MRLERERRYDSPISRTGHPGEIVHADGRVELADASALAASPLWN
                     EDLAPVPVARRNWTTYNFAALWISMAHCIPTYMLASGLMNSGMDWRQALFTILLGNTI
                     VLAPILLNSHPGTKYGIPFPVFARAAYGTAGSNLPALMRALVACGWFGIQAWIGGEAL
                     HTFFAAVIPGWPTLLGAGFSGHTTTEWLSFLMFWGLNVFVIYRGMDLLRKVENWAAPY
                     VLVVTLVLLVWAVREAKGLGPLLAQPGKFRTFAEFFPVFVPSLTAMIGFWATLSLNMP
                     DFTRFGRSQKEQVVGQVVALPTTMFAFAAMGVLITSASAIIFGEAIWDPVKLVGKFTN
                     PLVVAVAMFSVVVATLAVNIAANVVSPANDFANAVPKLISFKTGGLITGLIGIAMQPW
                     RLLADPKGYIFTWLLGYSGGLGSIAGVLVADYWIVRKKSLDLKALYLKDGAYGGWNPA
                     AVVATFAGCFLAWGGLVIPFLRPLYDYAWFVGFFAAGGLYALLMRVRPLEPPPAA"
     gene            10677..>11513
                     /locus_tag="PLB01_10450"
     CDS             10677..>11513
                     /locus_tag="PLB01_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77765.1"
                     /translation="MRFKAALAALALAALPLAADDLETAVSRMSRVGFSRSPSFSPDG
                     KTIAFVSNLSGLPQVWTVPAAGGFPRMVTSFDDPVGGVEWSPDGAWIAFSLAPGGGMN
                     AQVYLVKPDGTGLKRLTDGGKETNRLGNFRHDGKRLILGSNRRTGIGIDAYIYDVEAG
                     RLDLVSEIAGTGGIEDLSRDGKRALLNRLRYRGSNDLFLVDLATKKETLLTPHEGPGS
                     FDGVFSPDGKVVWVSSNGGRDRLAFGRIVIAADGRPGPIEIVAAREDAELQSFAIDDK
                     GEK"
BASE COUNT         1789 a         4087 c         3881 g         1756 t
ORIGIN      
        1 gcttccgcga ggcgatgcac ttcggcggcg ggaagcgccg cgggggcgcg cgtctcgagc
       61 ggcggcgcgc ccgcgcggtt cagctcgacg tcgcgcggga agtacgcggg aacttccggg
      121 aggtcggcgg tcatgagccg cacgaagtct tccctcggca tcgggcggag cgcgtagttc
      181 gtccggcgct gcacgccgat cgtcgaggag gtctcgctcg agatgttccg gccgcagagg
      241 gagcccgcgc cgtgcgccgg gtagacggcg acgtcgtcgg gcagcgtgag gagcttgccg
      301 tgcagcgagt cgtacagcgc gccggccata tcctgcgggg tgacgccgcc cggcgaggcg
      361 gcgagatcgg gccggccgac gtcgccgatg aagagcgtgt cgcccgtgaa gacgaggcgc
      421 ggctccgggt cgaggacgag gatcgagacg ctctcgggcg tgtggccggg cgtctcgagg
      481 aaccgcagga ggatgccgcc gagccggatc tcctcgccgt cgcgaacgcc gcggtgcggg
      541 aacgccgcgc gggccttgtc gccgaagacg atcgtcgcgc cggtccgcgc cgcgagctcc
      601 cggtggccgc tcacgaagtc cgcgtggagg tgcgtctcga ggatccactt gatcgcgagg
      661 ccctgcgcgc gcgcctcgtc caggtactcg tccacgtcgc gtcgggggtc gacgacggcc
      721 gcttccccgc cgtcgccgat caggtacgaa gcgtgcgcga ggcacccgag gtagaactgc
      781 ttgaaataca tctgaaagaa gagattatct cgctttcgga gaggcataat taccgttcta
      841 agaaagcgcc ttgacggcct gcatctgagc cgacagcgag aaggaaggcc ctgcgtgagc
      901 acagtctccg aagcaaagaa ttccccggtc tcttccgccg acgtccgggc caagcacaag
      961 gaattcctct tcccgtcggt cacgaactac tacaaggaat ccgtcgtcct cgagtccggc
     1021 aaggggatgt acctcaagga cgccgacggg aagcgttacc tcgacttctt cggcggaatc
     1081 ctcacggtct ccgtcgggca cgcgaacgac aaggtgaacg cggccgtcaa ggcccaggtg
     1141 gaccgcctcg gccacgtctc gacgctctac ccgacgctgc ctatcgttca actcgccgag
     1201 actctcgcgc ggatcacgcc gggccggctc cagaaatcga ccttcacggg ctcgggcacc
     1261 gaggccgacg agaccgccgt cgtgctcgcc caggtctaca cgggcaggct cgagatcatc
     1321 gcgctgcgcc acggctactc cgggcgctcg atcctcgcgc agtcgctcac ggcgcacgcc
     1381 acgtggcgcg cggtccctac ccagatcgcc gggatcaagc acgcgccgtc gccttactgc
     1441 taccggtgtc cgctcaagct cacgtacccc tcgtgcgggg tcgcctgcgc gaaggacctc
     1501 gaggagctca tcaagacgac gacgacgggc cagatcgccg gcttcctcgc cgagccgatc
     1561 cagggggtcg gcggcttcgt cacgcccccg aaggagtact tcgagatcgc cgtcgggatc
     1621 gtccggaaat tcggcggcgt cttcatctgc gacgaagtgc agaccggctt cgggcggacg
     1681 gggacgcact ggtacggcat ctcgcactgg aacgtcgagc ccgagatcat gacgatggcc
     1741 aagggcgtcg cgaacgggat gccgctcggg gtcacgatcg cgactccgga gatcgcggac
     1801 tccttcaagg ccctcacgat ctccacgttc ggggggaacc ccatctcctc gacggccgcg
     1861 aacgcgacga tcgccgtcat ggaggagatc gatgcgccga agcgcgcggc cgagatgggc
     1921 gcgcgcctga gggccgggct cgaaggcctc aagaagaagt acccgaagac gatcggcgac
     1981 gtgcgcgggc tggggctcat gcagggcctc gagctcgtgg cggacgagac ggtgcaggac
     2041 cggacgcccg ccccggaaaa ggcggcccgc ctcttcgagg agacgaaggc ccgcggcctc
     2101 ctgatcggca agggcggcct ctacggcaac gtcatccgga tcgccccgcc tctcatcgtc
     2161 tccgcgtccg aggtggacga cgccctaaga acgctcgacg agtccctcgc cggctgggcc
     2221 tgaatttccc gtattatcct gagcatcatt cgcaataccc ttttcgcccg ccgagtcgtc
     2281 gtgtgatcgg aacgaagctc gcggaccggt acgaaatcct ctccgagctg gggcgcggag
     2341 ggatgggagt cgtctaccgc gccaaggatc ccgtcctcaa ccgcgacgtc gcggtcaagc
     2401 tgatcccgcc cggaaacctc acgaaggacg ccgaggagcg cttccagcgc gaggcccaga
     2461 tcgtcgcgca gatggaccac ccgtccatcg tcccgatcta cgacctcggc cgccacgagg
     2521 ggtcgctctt cttcgtgatg ccggtgctcg cgaacacgaa cctccgccag cttctccgag
     2581 agggctcgct ccggctcggc gacgtgctcg acgtggggac gcaggtcgcg gacgccctcg
     2641 actacagcca tacgcgcggc atcgtccacc gcgacatcaa gcccgagaac atcatgacct
     2701 cgcgcgacga cacgggccac atccgcgcgc gcgtgatgga cttcggcctc gcgctcgcga
     2761 cgagcgagga ccgcctcacg aagaccggga cgctcgtcgg gaccgtcgtc tacttcagcc
     2821 ccgagcaggt cacgtcccgg tcgttcgacg ggcgctcgga cgtctacgcg ctcggaacgg
     2881 tgctctacga gtgcctcgcc ggagagccgc cgttcacggg cgaggtgcag tccgtcctct
     2941 accgcgtcgt ccacgagttg ccgcagccga tgcggagcgt cggcgccgac gtgagcgagg
     3001 agctcgaggc cgccgtcctc cagtgcctcg cgaaggaccc ggagaagcgg ccgaagcgcg
     3061 ccgggcacct cgccgaggcg ctgcgccggt accggagcaa gctgcgcgag gaggacttcg
     3121 ggaagtcggt cgtcttcacc gccagccgga tgctcccgcg ccccgccgtc gcggcgccct
     3181 tcatcgggcg cgaaaaggag atggccgagc tgcagcgccg gctccacgcc gcactcgggg
     3241 gcgagtgcga gttcgtcgtc ctcgcgggcg agcccggcat cggcaagagc cgcctcgtgg
     3301 aggagctcaa ggccctcgcg cgggcccgga agatccgcgt cctctcgggg cgcttcgtcg
     3361 agcacgaccg cgcgttcgcc caccaggggt tctgcgagct gatccaggac tacttccggt
     3421 cgaaggacac gggcctctcg gagtcctcgc gtcccgactt cacggacctc gcgggcgacc
     3481 ttctcgcgct cttccccgtc ctttcggaga tcggcgagct gcgcgccgcg gccagcagcg
     3541 actcgggagc cgtcaagccc ggagacgggc gcaaggccga ggacaagatc gcgatcttcg
     3601 agctcatcgc ccgtaccctc acgcggatcg cgggcggcaa gccgctgctc ctcgccctcg
     3661 aggagctgca cggggcgcag ctctcgatcg aggcgctcca gtacgtcgcg cgccgcctcg
     3721 gcccgacgcc gacgctcatc gtcgggacgt accgccagac ggaggtcgac aagcggcacc
     3781 cgctcgtgaa gatgctcgag tcgttccgcg gcgacccccg cttcgccgcg ctcacgctcg
     3841 ggccgttgac ggcgtccgag caccgtgccc tcgtggagct ttccgcgggc ggcggcaagc
     3901 tcaccgacga cctcgcaacg cggctcctcg cggcgacgga gggcaatccc ctcttcacga
     3961 aggagctcgt ccggtccctg ctcgactccg gcggcatcgc ccgcgacgac tccggcgccc
     4021 tgaacctgtc cggggcgacc ggtatctcgt ccgacgccct gccggagacg atccagcagg
     4081 cggtcgaagc gcggatcgag cggctgcccg aagaagtgcg cgacgtcctg tccgtggcgt
     4141 ccgtcctcgg aaagtccttc gacttcaagg acctcgagac gctcgccgag gacgccaagg
     4201 gtctcgacga cgccgtcgac cgcctcgtga aggacggcct cctcgaagaa gagcgcgagt
     4261 cgcgcggcga ccggctcgcc ttcgcgagcg gcatcgtgcg cgacgtcctc tacggcgcgc
     4321 tctcgaggcg caagcgccgc acgctccacc gttcgtacgc cgacctcctc gagaagcgcc
     4381 accagggccg gctcgaccgc gtctaccccg agctcgtcca ccacttctcc gaaggcgacg
     4441 tgcccgagaa ggccgtcgac tacagcctga agctcgcgaa gaagtccctc gacgccttca
     4501 gccccgagga ggccgtgcgg gtcgcgaaga cggccctcga ctacctctcc gacgaggaat
     4561 gggaaggcga caagtccctc gagggcgacg cgcgcctcct gctcgcccag gcctcgcgta
     4621 tgacgggcca cgccgacgcc gccctccgcg aggcggaggc ggccgggaag atcttcgagc
     4681 gtgagaagca gcccgtgagg gccgtcgagg cggtcctctt cgccgccgac acggcctgga
     4741 acgggcgaaa ggtggacgag gcccgccggc tcgtggagcg cggcatcgaa gcggcggccg
     4801 ccgcgcagga cacgacacac ctgccgaagc tcctttcgct cgccgcgacg gtcgcgaacc
     4861 tgcgcggcga ctacgcacgc gccgcggcct acggctcgca gctcgagcgg ctcggcggcg
     4921 agtcgaagaa ggctgccgag gtcatcgcgt ccggcggccg cctcgtggtc gccatgacga
     4981 gccccgtggc ggcggtcgag cccgcggcgg cgcacatcgt ggaggagacc gaggtcctcg
     5041 gcacggtctt cgagacgctg ctcgcgaccg acgccgacgg ccggatcgtg cctgctctct
     5101 gcgaggagtg gtccctcacc gaccacggga agtcggccct gctgcgactc cggcacgggg
     5161 tgcgtttctc cgacggctcc cccctgacgg ccacggccgt caagacctcc ctcgagcgtg
     5221 ccgtccgcgt cagggacaag gggatgccgg cggccctcgc ggcggtccgc ggcgccgccg
     5281 agcaccggaa aggcgaggcc gccggcgtct cggggatccg ggtggtctcg gaggacaagc
     5341 tggaattcga cttcgtggag ccgctcccga tctttccggc tctcctgtcc gaccacgtcg
     5401 cggccgtcgc tcgcgtcgtg ccgggcgaga acggcgccga gaccgcggtc gggacgggcc
     5461 cgttcgtcat tgcgcagcgg accgcggacc gtatcgtcct cgagcgcaac ccgcactact
     5521 ggaaagactc tgcgctcctg gacggaatcg agttccggac cgggatgaag gcctcggcga
     5581 tcgtctcggg cctgcgcacc ggagaaatcg agcttgcgcg cgacctctcc ccccaggacc
     5641 tcgacgcgat cctccgggag ccgcgattgc gcgcagggct cgtcgagacg ccgaagaagg
     5701 ggacctactt cgcgctgttc aactcgaaga gcaagctcgg cgggaacgtc gcgctccgac
     5761 gcgccctcgc gggcgtgacc cgcagccagg acttcgtgtg ggccgcgctc ggccgtttcg
     5821 ccgtcccggc gacggggatc ctgccgcccg gcatcctcgg ccacgacgcg ggccggcgcc
     5881 ggcccctcat ggcgcgggac gaggcgctcg ccctcctcga gggcgccggc ctgatgccgc
     5941 ccatcaccct cacggcggcg gtccatccca tcctgcgcga ccgccacaag gcgctcacgg
     6001 cggcgctctt cgcgatctgg gaggagctgg gcatcgaggt ccgggtcacc acgaccgaca
     6061 tgaagtcgta cctcgaggcc caggctgcgg cgacggtcga cctcctcatc tcccggtgga
     6121 tggcggacta cgaggacccg gacgacttca cgttcaacct cttccgctcg ggcgtcggat
     6181 acttccggtc cttcttttcg tcggatgaga ccgaccgcct tgccgaggag gcgcggatcg
     6241 agagccgccc ggcgatccgc gaaggtctct acaggaaact cgaaggcctc cttctcgact
     6301 cgcccgtcgt cattccgctc ttccacgagg tcgactaccg gatcgccgga cccggcgtgc
     6361 gcggagtcgc gctccggagc agcccgccct tcgtcaacta cccggagatc gggaagacgg
     6421 aagcgccggc cgcccacgcc gcgcccgagt ggggaggcgg cgtcgtccac gtgcccatcg
     6481 gcggcgtcgt gaccgagttc gatcctgcca cgatggcgat cctcgagcag gccgaaacga
     6541 tgcccctcgt cttcgagacg ctcacccgcg acctcgaagg cggccgcgtc gtcccgtggc
     6601 tcgcctcgga ggtgattccc gaggagggcg gagcgcgctt ccgcttccgc ttgaggcggg
     6661 gggtgcggtt ccacgacgga cgggccgtct ccgcgcgcga cgtccgctac accttcgagc
     6721 gcatcctcca gaaagggacg agtgccggaa agttcctcct cgccccgatc cggggcgccc
     6781 agggcctcat cgacggcaaa gcttccgacc tcgagggctt ccacgtcgtc agcccttcgg
     6841 agttcgtcat cgagctggag aagcccctgg cgttcttccc ggtcctcgtt tcgtggcccg
     6901 gcgcgggcat cgtgccggag agcaccaccg cgatcggaga aagctggcgg aagggctgca
     6961 tcggaaccgg tccgtaccgc gtggcgagct tcgagcccgg caagcggctc gagctcgagc
     7021 ggaaccccgc ctactggcgc gagggcttcc cgaagaacga cggcgtcgtc ttccgtttcg
     7081 gcgtgccccc cgaggagatc cgcagcgagt tcctcgcggg ccggttctcg atcgcgtcgg
     7141 acctcctccc cgccgacgcc gaggccctgc gccaggatcc gcggttccgc gcgacctacc
     7201 gcgaaagccc gagcctcgtc acgtacttcg ttacgttcaa catccacaag ggtgtcttcg
     7261 cggacgcgcg ggcgcggcgg gccgtcgccc aggggatcga cgccgcggcg atcgtccggc
     7321 gcacgctcgg ccggcacgcg atccccgcga acggcctcat cccgccgggc cttctcgggt
     7381 acgtcacgaa accgaggagc cacccccagg cgtcggggca ggtttcgggc gcccacacgg
     7441 tctcccgcga gacgaccgag ttcacggcgg tcgtgaatcc catcatgttc ggcgagttcg
     7501 gcggcttcac gaaggagctc acgcaggcct tcaaggagat ggggtactcc atccgcgtcg
     7561 tgaacaagac gatggcggag ttcctcgacg cccagcacaa cgcgccgacc gacgtcatga
     7621 tcgggcggtg gatcgccgac tacccggacg ccgacacctt cgcgcaggga gtcctccact
     7681 cccgcgaagg agtcaacggc cgctactgcg ggatgccggc catcgacgcc ctgaccgagc
     7741 gcgcccgcgg cgagatcgac ccgcgcaccc gggagtcgat ctaccgcgag gtcgaagaca
     7801 tcgtggcccg cgaggcgctc atgatcccgc tcttccacga gcaggtgtac cggttcgcgc
     7861 ggcccgaggt cgaaggcctc tccgtcggct tcggccagcc gatcgtcctg tacgagaacc
     7921 tttcgatcaa gagctagggg aatccgctac cgaacgcgat cgttcgcggg cgtcggctcg
     7981 ctcgccgcgt agccctcggc ctcgtagccg ccgtggtact cgccggggtt cggcctccgg
     8041 atctgcgact gcgggatcac ccggtcgcgc cgcagcccgc ggaagtcctc gagcgacgtc
     8101 cagccccgcg cggcgttctt ctcgaggaac gcagccatcc cgtccttgag cgcccggatc
     8161 acgttcgggc cgatcgcgtg gtcgagcatc gcggccgtgc agacctgaac ggtgccgcag
     8221 ccgaggagga agtagttgag ggcgtgcgag aagtccgaga cgccgccgat gcccgagaac
     8281 gaccggtccg ggaacgactg cgtgagctgc gccatcttcg cgagcgccag cgggaggatc
     8341 gccgggccgc cgaggccgcc cgaggacgtg tatccgtcca cgtgcatctc gaagtcgagc
     8401 gtctcggggt cgacgagcgg aatggacggg aacgtgttcg acgaggaaat cgcgtccgcc
     8461 ccgccccgga atgccgcgcc cgcctcgacc gcgatgtcgc tcgtggacgg cgtcagctta
     8521 gcccagacgg gcactttcgc gacctccttg acggctttcg tgacggtgga cgtcaggacg
     8581 ctgtccttcc cgatgttcga gcccatgtcc tcgcggtcca tgtgcgggca cgagaagttc
     8641 agctcgaggg cgtcgacgcc ctcgtcctgg caggtcttcg cgaggagctg ccagtttccg
     8701 agctccttgt cgttccccga gcccgccatg atcgaggcga cgagaacctt tccggggtgc
     8761 gccctcttga tccgcgacag gcgcccgacc caccagtcga gaggcttgtc ggagatgagc
     8821 tcccagttcc acgacgaatg gagaacggag tcgggccggg gcttcatcga gatgcgcggc
     8881 gactgcgtgt ccgtcctcag gaacttcgtc ttgggccccg ccacgttcac gaccgggtgg
     8941 aggccgatcg tcttcgtgac gaccccgccc cagccggcct cgaaggcccg gaggatgttc
     9001 acgtcggact ccgtcggcgg ggccgacgcg aggagaaacg ggttttcgaa ccggatgccc
     9061 gtgaagacga ccgaaagatc ggcggccatc gcgtcctccc ctgtgaatcc cggaaaaggt
     9121 gaacccccag cataagacga ttgagactcg aacgcgagcg gcggtacgat tcgcccatca
     9181 gcagaaccgg acatccgggc gagatcgtcc acgccgacgg gcgggtggag ctggcggacg
     9241 cgtccgccct cgcggcgagc ccgctctgga acgaggacct cgcgcccgtt cccgtcgcga
     9301 ggcgaaactg gacgacgtac aacttcgccg cgctctggat ctcgatggcg cactgcatcc
     9361 cgacgtacat gctggcctcg ggcctcatga actccgggat ggactggcgg caggcgctct
     9421 tcacgatcct cctcgggaac acgatcgtcc tcgcgccgat cctcctgaac tcgcacccgg
     9481 gcacgaagta cggcattccc ttccccgtct tcgcgcgcgc ggcctacggg accgccggat
     9541 cgaacctgcc ggcgctgatg cgcgcgctcg tcgcgtgcgg gtggttcggg atccaggcgt
     9601 ggatcggcgg cgaggccctg cacacgttct tcgcggcggt gatcccgggc tggccgacgc
     9661 tcctgggggc cggcttttcc ggccacacga cgacggaatg gctctcgttc ctgatgttct
     9721 ggggcctcaa cgtcttcgtc atctaccgcg ggatggacct cctccggaag gtcgagaact
     9781 gggcggcgcc gtacgtcctc gtcgtcaccc tcgtcctcct cgtctgggcg gtgcgtgagg
     9841 ccaagggcct tgggccgctc ctcgcccagc ccgggaagtt ccggacgttc gcggagttct
     9901 tccccgtctt cgtcccgtcg ctcacggcga tgatcggttt ctgggcgacg ctctcgctca
     9961 acatgcccga cttcacgcgc ttcggcagga gccagaagga gcaggtcgtc ggacaggtcg
    10021 tcgcgctccc gacgacgatg ttcgcgttcg cggcgatggg cgtcctcatc acgagcgcga
    10081 gcgccatcat cttcggggag gcgatctggg acccggtcaa gctcgtcggg aagttcacga
    10141 atccgctcgt cgtcgcggtc gcgatgttct cggtcgtcgt cgcgacgctc gccgtcaaca
    10201 tcgcggcgaa cgtcgtctcc cccgccaacg acttcgcgaa cgccgtgccg aagctcatca
    10261 gcttcaagac gggcggcctc atcacggggc tcatcggcat cgcgatgcag ccctggcgcc
    10321 ttctcgcgga cccgaagggc tacatcttca cgtggctcct cgggtactcg ggcgggctgg
    10381 gttccatcgc gggcgtcctc gtggccgact actggatcgt ccggaagaag tcgctcgact
    10441 tgaaggcgct ctacctgaag gacggtgcgt acggagggtg gaacccggcc gcggtcgtcg
    10501 cgacgttcgc gggctgcttc ctcgcgtggg gaggcctcgt catccccttc ctcaggcctc
    10561 tctacgacta cgcgtggttc gtggggttct tcgcggccgg cggcctctac gcgctcctga
    10621 tgcgcgtccg cccgctcgaa ccgccccccg cggcctgacg ccgcctagaa tcgcccatgc
    10681 gattcaaagc cgccctcgct gcgctcgccc tcgccgccct tcccctcgcc gccgacgacc
    10741 tcgagaccgc cgtgtcgcgc atgtcgcgcg tcggcttctc tcgctcgccg agcttctccc
    10801 ccgacgggaa gacgattgcc ttcgtctcga acctcagcgg cctgccacag gtgtggacgg
    10861 tccccgccgc cggcggcttc ccgcggatgg tcacgtcctt cgacgacccg gtaggaggcg
    10921 tcgagtggtc tccggacggc gcatggatcg cgttcagcct cgcgcccggc ggcggcatga
    10981 acgcgcaggt ctacctcgtg aagcccgacg gcaccggcct caagcgcctt acggacggag
    11041 ggaaggagac gaaccggctc gggaacttcc ggcacgacgg gaagcggctg atcctcggct
    11101 cgaaccgccg cacgggaatc ggcatcgacg cctatatcta cgacgtggag gcggggcgcc
    11161 tcgaccttgt gagcgagatc gcgggcacgg gcggcatcga agacctctcg cgcgacggaa
    11221 agcgggccct cctgaaccgg ctccgctatc gcggaagcaa cgacctcttc ctcgtggacc
    11281 tcgcgacgaa gaaggagacg ctcctgacgc cgcacgaggg accgggatcc ttcgatggag
    11341 tcttttcgcc ggacgggaag gtcgtctggg tttcttccaa tgggggaaga gaccggctcg
    11401 ccttcggcag gatcgtgatt gcggcggacg gcaggcccgg ccccatcgag atcgtcgcgg
    11461 cgcgcgagga cgcggagctg cagtcgttcg cgatcgacga caagggcgag aag
//
LOCUS       DAOMFY010000111        11507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1752_length_11507_cov_6.702847, whole genome shotgun sequence.
ACCESSION   DAOMFY010000111 DAOMFY010000000
VERSION     DAOMFY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_11507_cov_6.702847"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLB01_10455"
     CDS             complement(<1..710)
                     /locus_tag="PLB01_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQQ77766.1"
                     /translation="MTAPRSARELALGVLLRVDKGAHAAPLLDARGRELDTRDRDFLR
                     ALVKKTLRGAIRLDHVVQRHLKQPAASLDSPVLAALRLGAAQLLLMDRVPAHAAVGET
                     VAAVRVFAPKAAGLVNAVLRRVAEREARPGAVRLPPGADPLTRLALETSHPEWLVRRW
                     VAAFGEPAARATMEADDVDTPTDLLADPRAGSVDEILAALAADGVTAERSPWAPLALT
                     VTGGSAAAHPLVSGGALAV"
     gene            complement(707..1645)
                     /gene="fmt"
                     /locus_tag="PLB01_10460"
     CDS             complement(707..1645)
                     /gene="fmt"
                     /locus_tag="PLB01_10460"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQQ77767.1"
                     /translation="MRTVFFGSPTFAVPTLEALRSAGHDVVLVVSQPGKPVGRKAEIT
                     DPPVAALAKSLGMHVFQPPTLKDDAAFARLAEAHADVFVVAAYGKILAQRVLDLPRLG
                     CLNVHGSLLPRWRGASPVQASILAGDEKTGVSIMKMEAGMDTGPVYTTRETEIGEEED
                     SASLGARLAAMGADALVETLNSLEGEEGRTPVPVPQDEARATYCPKIAREDARVDWTR
                     PAAELVRRARAFTPWPGLFTTRRKTRVKLAGLSLEAGRPGARTGDVLEAGSRVVVACG
                     EGAVAVATLQAEGRKALSAADFVRGERVAAGETWGE"
     gene            1737..2024
                     /locus_tag="PLB01_10465"
     CDS             1737..2024
                     /locus_tag="PLB01_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQQ77768.1"
                     /translation="MAGMTKSQIAEHMAEKTGLTKKNAALFLEELANLAYKQAKNTFT
                     FPGVGKLVLVNRKARMGRNPATGEAIKIPAKRVVKFRVAKAAKDAILGAKK"
     gene            complement(2115..4526)
                     /locus_tag="PLB01_10470"
     CDS             complement(2115..4526)
                     /locus_tag="PLB01_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQQ77769.1"
                     /translation="MATRAAEPPSALARRRDEFLGILLAAAGLLLVAALVSYHPNDPS
                     LFSAVNDSGIRPRNWAGRVGASFADGSLQFFGLAAFVAPLALLALGWSRFRGRPLEAA
                     GTKGLGLAIVVLTAAPLAHLAFGKPQAFGGGLDAGGFVGDLLGSGLVAALNPPGAAIL
                     LVAAFLIGLLLATSLSLGEGVSGASVRASGWWRGLVLERQRRRELASREQQRREVVKK
                     HLERAKEETEKPPARPPEPEAEVVYIPSVSALRVKEKAGAGKFSIRKATPDGPALRPV
                     ARPAQMAKKPDVPQRVLPFPKSRDASGWVFPPEKLLRAAPKRDRERDREEYRQTMELI
                     AAKCLEFGVEGVVDAYTPGPVVTTYEFKPSAGVKVSQVASLENDLALALAAEKVRIER
                     MPGRAAIGIEVPNRNRDIIALREVVESEKFRRSPSLLTIALGIDVHGEPVVADLARMP
                     HLLVAGQTGAGKSVGVGGMITSILFKARPDEVKFIFVDPKMNDMKDYEDLPHLLVPVI
                     TDPKKAANALKWAVDEMEGRYKLLSEWPGVRNIEQYNAAIRDPKALAEVREKLGEKLK
                     ADESGVVSPMPYIVIVIDELADLMMTAPREIEESVARLAQKARAVGVHLILATQRPSV
                     DVITGTIKANLPCRISYTTRTKIDSRTILDSMGAEALLGAGDMLFLASGTSYLQRVHG
                     GFIGGDETKEICRFLKKQGKPIYDASVIEDREAQSAAAGARSGEGDNDPMYDEAARLV
                     VRERMASISFLQRRLEIGFSRAGKLIDMMQRDGIVGPPAGGSRSREILVPTDYFEEID
                     RNRRS"
     gene            complement(4526..5419)
                     /locus_tag="PLB01_10475"
     CDS             complement(4526..5419)
                     /locus_tag="PLB01_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQQ77770.1"
                     /translation="MSVSVGQAVLLGAVQGLTEFLPVSSTAHLALAQRFLPGFAQPGI
                     LFDVLLHVGTLAAVVVFFRERLGRTFAGLASSDPASRRAAWKLAGLLILAVALTGAVT
                     LPLKSLAVENMGAFRMMGGALLAMAVLLLVAQRFGTRLGEGGRTLDEMRPRDAALVGA
                     LQSLSAIFHGFSRSGNTIATGLFCGLSRRAAAEFSFLLSIPTILAAAAVENLHAYRHH
                     TGPLSDGSSLPAYGAGMLVSAVVGYFAVGFLMKVIVAMRLTPFIAYCALLGATLLVFG
                     GRLESGSVGGATLATLAGGVR"
     gene            complement(5429..6235)
                     /gene="rsmA"
                     /locus_tag="PLB01_10480"
     CDS             complement(5429..6235)
                     /gene="rsmA"
                     /locus_tag="PLB01_10480"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQQ77771.1"
                     /translation="MRASSAARPRPRWGQHFLVNAGTVRKILDALGAGEGETVVEIGP
                     GRGALTVPLLDRGVRVLAFEIDPPLAARLSADHSERTLFVETEDALHADVGAALERIG
                     ATPPVPLVGNLPYESATPMIRAFVRRPDLYSRLVVMIQREVADRLVSPPGGDAYGFLT
                     LDVGAHASARKLFDVGRGEFSPPPKVLSSVVELVPHPPGAGIDAALKVASAGFTSRRK
                     TLVNALTPLWGRERAAGAVAEAGLPPTARAETLGLDVFRRLSLSLGAPVR"
     gene            6307..6636
                     /gene="trxA"
                     /locus_tag="PLB01_10485"
     CDS             6307..6636
                     /gene="trxA"
                     /locus_tag="PLB01_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQQ77772.1"
                     /translation="MAGNVQELTESSFESTVLKSAKPVLVDFWAVWCGPCRQVAPIVE
                     SLATKWGDKVTVTKLNVDDVPSVAERYGIMSIPTLMLFKGGQIVERVVGVSPQASLEK
                     KFEPHLN"
     gene            complement(6694..7086)
                     /locus_tag="PLB01_10490"
     CDS             complement(6694..7086)
                     /locus_tag="PLB01_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77773.1"
                     /translation="MTGTPPLRTLAASLVIAAVLAGPGSAATKKKGKATSTPAPTPAP
                     TAVPTPTPTPTPELHKAAGSCLRYEAGAYIMLSEVGQPGRAFRIDSGTDITARPRRGA
                     RLRILYVDSPDGPIARKIMPGPVEEKKN"
     gene            7227..8270
                     /locus_tag="PLB01_10495"
     CDS             7227..8270
                     /locus_tag="PLB01_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQQ77774.1"
                     /translation="MTTPSNTATPPPAAPGLDPAALTERIREKSLTVGALLTEIRKVI
                     VGQSALVERLVIGLLADGHLLLEGVPGLAKTLAVKTLARGLDASFSRVQFTPDLLPAD
                     LVGTLVFNPKTQEFVARRGPIFANVVLADEINRAPAKVQSALLEAMQERQVTVGDTTA
                     PLPDPFLVLATQNPIEQEGTYPLPEAQVDRFLMKLKVGYPTKAEERQILERMGETTEA
                     AASASVRPILTPEAIRELRVLVRGIYADERIKGYLVELVAATREPKAYGLPDLADFIS
                     YGASPRATLALLGAARGRAFLAGRGYVIPEDVKDVAPDVLRHRVLLSYEAEAENVSSD
                     QVVKRILERVEVP"
     gene            8286..9170
                     /locus_tag="PLB01_10500"
     CDS             8286..9170
                     /locus_tag="PLB01_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQQ77775.1"
                     /translation="MLPREIVKKLHRIRIRTNRLVEAGVGGEYHSVFRGRGMEFSEVR
                     EYVPGDDVRTIDWNVTARTGVPHVKKFVEERDLTVLLLLDLSASQGFGSKYLTKRELM
                     AEIAALLSFAAVKNNDRVGALLFTDRLERYHPPRKGVDHALAITRDALSLEPGGAGTD
                     IALALRSAAGLLKQRSVVFLLSDFLAAGYEKALKTLNKRHDVVAIPVSDPREREIPAS
                     GLVRLRDAETGEARVFDASDPAFREAWGLRPAELPEPAPIFRAAGIDSIPLRTDASYE
                     LPLVRFFKEREKRKAAGR"
     gene            9167..10273
                     /locus_tag="PLB01_10505"
     CDS             9167..10273
                     /locus_tag="PLB01_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77776.1"
                     /translation="MKRILLAPERTRAALATGVLLAAGFGVFSSSAEEGGLPAGTAGA
                     AAEAPSSPSKKIFSSAVADKTSVSIGDRVVVTYSAKLPAGATLSLDSLVTPAPEEGAR
                     PPGGAVLEFELPSEPTLKKSGDFFEWSQSVALFPFVAGTVQVPGPHYTFEESSSGRRL
                     DVRPPSVELTVASRLPKDQKPEQIAPKADKGIRIPPIPAKFWAALGAVVALAAGLVWW
                     GILRRRRQGDLGVPASPPVPPGEELRLELDRLAPAVDTLGDDARGFYSELTHAVKRYL
                     ERTTGDPVLEWTTFETVRRFREKGFDLPADVALPELLASADRVKFGKGAATRDDAREG
                     LARARRLHDHVEAREAARRAAEAAAAASAKRKAS"
     gene            10270..11334
                     /locus_tag="PLB01_10510"
     CDS             10270..11334
                     /locus_tag="PLB01_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ77777.1"
                     /translation="MTIPAALRDFVASHVPPGLSFKDPLFLLLLVLVPVAIALKILRE
                     RRGDGALVVSTLAPVARVVPSWRVRLRHLPFALALVGYAGLVVSLARPRLGLERTESW
                     TEGVDIFVALDASGSMAAEDFKPKNRFHVAKACTRAFIEGRPNDRVGLLVFAGRSRTV
                     SPLTTDRVMVLDRLGGLKLGDQGDGTAIGLALGNALARLKPSKAKSRVIVLVTDGGNN
                     AGEIDPDTAAGVAKALGVRVYTVGVGTNTGPVEIPIPMKDPETGRVVERRIRANVDVD
                     EALLQRIAKETGARYFRATDSQGLADTFATIDRLEKTEIKTTRYTRFREVFPEVARPA
                     ALCLALSALGALLLFPVAPR"
BASE COUNT         1742 a         4186 c         3976 g         1603 t
ORIGIN      
        1 acggcgagcg cgccccccga gacgagcggg tgcgccgccg cgctgccgcc cgtcaccgtg
       61 agcgcgagag gggcccaggg cgaccgctcc gccgtcacgc cgtccgccgc gagcgcggcg
      121 aggatctcgt cgaccgagcc tgcgcgcgga tcggcaagga ggtccgtcgg cgtgtcgacg
      181 tcgtccgcct ccatcgtcgc acgtgcggcc ggctccccga acgccgcgac ccagcgcctc
      241 acgagccatt ccgggtgcga cgtctcgagc gcgaggcgcg tgagcggatc ggcgccgggc
      301 ggaaggcgca ccgcgccggg cctcgcctcg cgttcggcga cgcgccgcag gacggcgttc
      361 acgagcccgg ccgccttcgg cgcgaagacg cggaccgccg ccacggtctc cccgaccgcc
      421 gcgtgagcgg gcacgcggtc catgagaaga agctgtgccg ccccgagccg cagggcggca
      481 aggaccggcg agtcgagcga ggcggcaggc tgcttgaggt gccgctggac gacgtggtcg
      541 agccggatcg ccccgcgcag ggtcttcttg acgagggccc ggaggaagtc gcggtcgcgc
      601 gtgtcgagct cgcgtccgcg cgcatcgaga agaggggccg cgtgggcgcc cttgtcgacg
      661 cgcaggagga caccgagcgc gagctcgcgc gcggaacgcg gggcggtcat tcgccccagg
      721 tctcgccggc cgccacgcgc tcgccgcgga cgaagtcggc cgcggagagt gccttgcggc
      781 cctcggcctg cagcgtcgcg accgcgaccg ccccctcgcc gcaggcaacg acgacacggc
      841 ttccggcttc gaggacgtct cccgtccgcg ctccaggccg gcccgcttcg agagagaggc
      901 ccgccagctt cacccgagtt tttcttcgag tggtaaagag cccgggccag ggggtgaagg
      961 ctcgcgcacg gcggaccagc tctgccgcgg ggcgcgtcca gtcgacccgc gcatcctccc
     1021 gggcgatctt cggacagtac gtggctcgtg cctcgtcctg tgggacaggc accggagttc
     1081 tgccctcttc accttcgagg gaattcaacg tctcgaccag cgcatcagct cccatcgctg
     1141 cgagccgcgc gcccagggaa gcagaatcct cctcttcccc gatctccgtt tcccgcgtcg
     1201 tgtagacggg gcccgtgtcc atcccggcct ccatcttcat gatcgagacg cccgtcttct
     1261 cgtcgccggc gaggatcgac gcctggaccg gagaggcccc ccgccagcgc gggagaaggc
     1321 ttccgtggac gttcaggcag ccgaggcggg gcaggtcgag caccctctgg gcgaggatct
     1381 tcccgtacgc ggccacgacg aaaacgtcgg catgcgcctc ggcgagccgc gcgaaggcag
     1441 cgtcgtcctt cagcgtagga ggctggaaaa cgtgcattcc cagcgacttc gcgagcgccg
     1501 cgacgggagg atccgtaatc tccgccttcc tcccgacggg ctttccgggc tgcgagacga
     1561 cgaggacgac gtcgtggccg gccgacctca acgcctcgag cgtcgggacc gcgaaagtcg
     1621 gcgagccgaa aaaaacagtg cgcacgcgca gagtgtatag acacgaaacg cagttttccc
     1681 tctagaatcc gggcattccc tcgcgggaac caagttacga gaaaggacgg aatcacatgg
     1741 ctggaatgac caagtcgcag atcgcggagc acatggcgga gaagacgggg cttacgaaga
     1801 agaacgcggc gctcttcctc gaggagctcg cgaacctcgc ctacaagcag gcgaagaaca
     1861 ccttcacgtt ccccggcgtc ggcaagctcg tgctcgtgaa ccggaaggcc cgcatgggtc
     1921 gcaacccggc gacgggcgag gcgatcaaga tcccggccaa gcgcgtcgtg aagttccgcg
     1981 tcgcgaaggc cgccaaggac gcgatcctcg gcgcgaagaa gtagcgcgga ggggttcggg
     2041 gaacccgcgc ttcgggttcc ccgattcccg cggccgaagg ccgcggagca gaaatccccc
     2101 ggagccggcc gcgatcagct cctgcggttg cggtcgatct cttcgaagta gtccgtcggg
     2161 accaggatct cgcggctgcg gctgcccccc gccggcggac ccacgatccc gtcgcgctgc
     2221 atcatgtcga tgagcttgcc cgcgcgggag aacccgatct cgagccgccg ctgcaggaac
     2281 gagatcgacg ccatgcgctc gcgcaccacg agccgggccg cctcgtcgta catcgggtcg
     2341 ttgtcgcctt ccccgctgcg cgcgccggcc gccgccgact gcgcctcgcg gtcttcgatc
     2401 acgctcgcgt cgtagatcgg cttgccctgc ttcttgagga agcggcagat ctccttcgtc
     2461 tcgtccccgc cgatgaaccc gccgtgcacg cgctgcaggt acgacgtacc ggacgcgagg
     2521 aacagcatgt caccggcccc gaggagggcc tcggccccca tcgagtccag gatcgtccgg
     2581 gagtcgatct tcgtccgggt cgtgtaggag atgcggcacg ggaggttcgc cttgatggtg
     2641 cccgtgatga cgtccacgga cggccgctgc gtcgcgagga tgaggtgcac gccgacggcc
     2701 cgcgccttct gcgcgaggcg ggcgaccgac tcctcgatct cgcgcggcgc cgtcatcatg
     2761 aggtccgcga gctcgtcgat cacgatcacg atgtagggca tcggcgagac cacgccggac
     2821 tcgtcggcct tcagcttctc gccgagcttc tcgcgcactt ccgcgagagc cttcgggtcc
     2881 cggatcgcgg cgttgtactg ctcgatgttc cgcaccccgg gccactcgga gagcagcttg
     2941 taccggccct ccatctcgtc cacggcccac ttcagcgcgt ttgcggcctt cttcgggtcc
     3001 gtgatgaccg gaacgaggag gtgcggaagg tcctcgtagt ccttcatgtc gttcatcttc
     3061 gggtcgacga agatgaactt gacctcgtcc gggcgcgcct tgaagaggat cgacgtgatc
     3121 atgccgccca cgccgacgct ctttcccgcg cccgtctggc ccgcgacgag aaggtgcggc
     3181 atgcgggcga ggtccgccac gaccggttcg ccgtgcacgt cgatcccgag cgcgatcgtg
     3241 aggagagacg gcgaacggcg gaacttctcg ctctcgacga cctcgcggag cgcgatgatg
     3301 tcgcggttcc ggttcgggac ttcgatcccg atcgccgcgc ggccgggcat gcgctcgatc
     3361 cggaccttct cggccgcgag ggcgagcgcg aggtcgttct cgagcgaggc gacctgcgag
     3421 accttgacgc ccgccgacgg cttgaactcg tacgtcgtga cgacgggccc cggcgtgtag
     3481 gcgtcgacga cgccctcgac accgaactcg aggcacttgg ccgcgatgag ctccatcgtc
     3541 tggcggtact cctcgcggtc gcgctcccgg tcgcgcttcg gcgccgcgcg caggagcttt
     3601 tcgggcggga acacccagcc ggaggcgtcg cgcgacttcg ggaacggaag gacgcgctgc
     3661 ggcacgtccg gcttcttcgc catctgggcg ggccgcgcaa cgggccgcag cgcgggcccg
     3721 tcgggcgtcg ccttgcggat cgagaacttc ccggcgcccg ctttctcctt cacgcgcagc
     3781 gccgagacgg acgggatata gacgacctcg gcctccggct cgggcgggcg cgccgggggc
     3841 ttctccgtct cctctttcgc cctctcgagg tgcttcttga cgacttcacg gcgctgctgt
     3901 tcgcggctcg cgagctcgcg gcggcgctgg cgctcgagaa ccaggccgcg ccaccagccc
     3961 gaagcgcgca ccgaggcgcc cgaaacgccc tcgccgagcg agagcgacgt cgcgaggagg
     4021 aggccgatca ggaacgcggc gacgaggagg atcgcggcgc cgggcggatt gagcgccgcg
     4081 acgaggcccg agccgaggag gtcgcccacg aagccgcccg catcgaggcc gccgccgaac
     4141 gcctgcggct tgccgaacgc gaggtgcgcg agcggcgcgg ccgtcaggac cacgatcgcg
     4201 aggccgaggc ccttggtgcc ggcggcctcg agcggcctgc cacggaagcg cgaccagccg
     4261 agcgcgagga gcgcgagcgg cgcgacgaag gccgcgaggc cgaagaactg gaggctgccg
     4321 tccgcgaacg acgcgccgac gcggcccgcc cagttgcgcg gccggatccc ggagtcgttc
     4381 acggccgaga agagcgacgg gtcgttcggg tggtacgaca cgagggccgc gacgagcagc
     4441 aggcccgcgg ccgcgaggag gatcccgaga aactcgtctc gccggcgcgc gagcgcgctc
     4501 ggcggctcgg cggcgcgggt tgccatcagc gaactccgcc ggcgagcgtc gcgagggtcg
     4561 ccccgccgac ggacccgctc tcgaggcgcc ccccgaacac gaggagcgtg gcgccgagaa
     4621 gggcgcaata ggcgatgaag ggggtcaagc gcatcgcgac gatcaccttc atgagaaatc
     4681 ccacggcgaa ataccccacg accgccgaaa cgagcatccc ggccccgtaa gcagggagcg
     4741 aagagccgtc ggagagcggc cccgtgtgat ggcggtacgc gtgcaggttc tcgacggccg
     4801 cggccgcgag gatcgtcggg atcgacagca ggaacgagaa ctcggcagcc gcgcggcgcg
     4861 agaggccgca gaacagaccc gtcgcgatcg tattaccgga gcgcgagaac ccgtggaaga
     4921 tcgccgagag cgactggagc gcgcccacga gcgccgcgtc gcgcgggcgc atctcgtcga
     4981 gcgtccgacc gccctcgccg aggcgcgtgc cgaaccgctg cgcgacgagg aggaggaccg
     5041 ccatcgcgag gagcgctcct cccatcatcc ggaacgctcc catgttctcg accgcgaggc
     5101 tcttcagcgg aagcgtcacg gcgcccgtga gtgcgacggc gaggatcagc aatcccgcga
     5161 gcttccatgc ggcgcggcgc gacgccgggt cggacgaggc gagcccggcg aacgtccgcc
     5221 cgagacgttc gcggaagaag acgacgaccg cggcgagcgt cccgacgtga agaagcacgt
     5281 cgaagaggat cccgggctgc gcgaagccgg gcaggaaccg ctgcgccagc gcgagatggg
     5341 cggtcgacga gaccggcagg aactcggtca gaccctggac ggcgcccagc agcaccgcct
     5401 ggcccacgga cacggacatc gacgaatttt agcgcacggg agcgcccagg gagagggaaa
     5461 gcctccggaa aacgtcgagg ccgagcgttt cggcgcgcgc cgtcggaggc aggccggcct
     5521 cggccacggc gcccgcggcg cgctcgcgtc cccagagcgg cgtcagcgcg ttcacgagcg
     5581 tcttgcggcg cgacgtgaag ccggccgatg cgaccttaag cgccgcgtcg atgcccgcac
     5641 cgggagggtg cgggacgagc tcgacgacgc tcgaaaggac cttcggcggc ggcgagaact
     5701 cgccgcggcc gacgtcgaag agcttcctcg cggacgcgtg ggcgccgacg tccagcgtca
     5761 ggaacccgta cgcgtcgccc ccgggaggcg agacgagccg gtcggcgacc tccctctgga
     5821 tcatcacgac gaggcgcgag tagagatcgg gccggcgcac gaacgcgcgg atcatcggcg
     5881 tggcgctctc gtacgggaga ttcccgacga gcggcaccgg aggcgtcgcg ccgatgcgct
     5941 cgagggcggc accgacgtcc gcgtgcagcg cgtcctccgt ctcgacgaac agcgtccgct
     6001 cggagtggtc cgccgagagc cgggcggcga gcggcgggtc gatctcgaac gcgaggaccc
     6061 gcacgccgcg gtcgaggagg ggaaccgtga gggcgccgcg ccccggaccg atctcgacga
     6121 cggtctcccc ctccccggcg ccgagggcgt cgagaatttt ccggaccgtc cccgcgttga
     6181 cgaggaaatg ctgaccccat cgcgggcggg ggcgcgccgc gcttgacgct ctcacgacgc
     6241 tgaccctacc atcgaaatcc gctccggccg cccggccgga gccacgccag aaaggaacgt
     6301 cccaccatgg ccggaaacgt ccaggagctc accgaaagct cgttcgaatc gaccgtcctc
     6361 aagagcgcca agcccgtcct cgtggatttc tgggccgtct ggtgcggccc ctgccgccag
     6421 gtcgcgccca tcgtcgagtc gctcgccacg aaatggggcg acaaggtcac cgtgacgaag
     6481 ctgaacgtcg acgacgttcc ctccgtcgcc gagcgctacg gaatcatgtc gattccgacg
     6541 ctcatgctct tcaaaggcgg ccagatcgtc gagcgcgtcg tcggggtgtc gccgcaggcc
     6601 tcgctcgaga agaagttcga gccgcacctg aactgagctg tcccggggga acccggcctc
     6661 gggttccccc gaaccccctc cgcggcagac ggcctagttc ttcttctctt cgacgggccc
     6721 cggcatgatc ttccgcgcga tcgggccgtc gggcgagtcc acgtagagga tccggagccg
     6781 cgcgccgcgg cgggggcggg cggtgatgtc cgtccccgag tcgatccgga atgccctccc
     6841 cggctggccg acctccgaca gcatgatgta ggcgcccgcc tcgtacctca gacaggagcc
     6901 cgccgccttg tgcaactccg gcgtaggggt cggagtcggc gtcgggacgg ccgtcggagc
     6961 gggcgtcggg gccggcgtcg acgtcgcctt tcccttcttc ttcgtggccg ctgagcctgg
     7021 tcccgcgaga acggccgcga tcacgagcga cgcggcgagc gtgcgcaggg gcggcgttcc
     7081 cgtcattccc ggaagaatag ctccggcatc cggcttgctc acaaccggat ttggagctcc
     7141 aaggtgccgt tccggcgccc cgcccgaggc tcgggccgcg accggtcggc gaagccagga
     7201 accggcgaga agggacacgc gacacgatga cgacgccgag caacaccgcc acgccgcccc
     7261 cggccgcgcc gggcctcgac ccggccgcgc tcacggagcg catccgggag aagtcgctca
     7321 ccgtgggcgc cctgctgacc gagatccgaa aggtgatcgt cggccagagc gctctcgtcg
     7381 agcgcctcgt gatcgggctc ctcgccgacg gccacctgct tctcgagggc gtgccgggcc
     7441 tcgcgaagac gctcgccgtc aagacgctcg cgcgcgggct cgacgcctcc ttctcgcgcg
     7501 tccagttcac gcccgacctc ctgcccgccg acctcgtcgg cacgctcgtg ttcaacccga
     7561 aaacgcagga gttcgtcgcc cggcgcggac cgatcttcgc gaacgtcgtc ctcgccgacg
     7621 agatcaaccg cgcgcccgcg aaggtccagt cggccctcct cgaggccatg caggagcgcc
     7681 aggtcaccgt gggcgacacg acggcgccgc ttcccgaccc gttcctcgtc ctcgcgacgc
     7741 agaacccgat cgagcaggaa ggcacgtatc ccctgcccga ggctcaggtc gaccggttcc
     7801 tcatgaagct gaaggtcggc tacccgacga aggccgagga gcgccagatc ctcgagcgga
     7861 tgggcgagac gaccgaggcg gccgcctcgg cgtccgtccg ccccatcctc acgccggagg
     7921 cgatccgcga gctgcgcgtc ctcgtgcgcg gcatctacgc ggacgagcgc atcaaaggct
     7981 acctcgtcga gctcgtcgcc gcgacgcgcg agccgaaggc ctacgggctc cccgacctcg
     8041 ccgacttcat ctcgtacggc gcgtcgccgc gcgcgacgct tgccctcctc ggagcggcgc
     8101 gcggccgcgc gttcctcgcg gggcgcggct acgtcattcc cgaagacgtc aaggacgtcg
     8161 ccccggacgt cctgcgccac cgcgttctgc tctcgtacga ggccgaggcc gagaacgtgt
     8221 cgagcgacca ggtcgtcaag cggatcctcg agcgcgtcga agttccctga ggccgccgac
     8281 gccgcatgct gccccgcgag atcgtcaaga agctccaccg catccggatc cgcacgaacc
     8341 ggctggtcga ggcgggcgtg ggcggggagt accactcggt cttccgcggg cgcgggatgg
     8401 agttctccga ggtccgcgag tacgtgcccg gcgacgacgt gcggacgatc gactggaacg
     8461 tgacggcgcg gaccggcgtc ccgcacgtga agaagttcgt cgaggagcgc gatctcacgg
     8521 tcctcctcct cctcgacctc tccgcctcgc agggcttcgg ctcgaagtac ctcacgaagc
     8581 gcgagctcat ggccgagatc gcggccctgc tctccttcgc ggccgtcaag aacaacgacc
     8641 gggtcggggc gctcctcttc acggaccggc tcgagcgcta ccacccgccg cgcaagggcg
     8701 tcgaccacgc gctcgcgatc acgcgcgacg cgctctcgct cgagcccgga ggcgccggca
     8761 ccgacatcgc gctcgcgctc cggagcgcgg ccggcctcct caagcagcga tcggtcgtgt
     8821 tcctgctctc ggacttcctc gcggccgggt acgagaaggc gctcaagact ctcaacaagc
     8881 ggcacgacgt cgtcgcgatc ccggtctccg acccgcgcga gcgcgagatc ccggcctcgg
     8941 ggctcgtgcg cctgcgcgat gccgagacgg gcgaggcgcg cgtcttcgac gcgtccgacc
     9001 ccgcgttccg agaggcgtgg gggcttcgcc ccgccgagct tcccgagccc gcgccgatct
     9061 tccgcgccgc gggaatcgat tccatcccgc tccgcacgga cgcgtcgtac gagcttcccc
     9121 tcgtgcgctt cttcaaggag cgcgagaagc gcaaggcggc gggaagatga agagaattct
     9181 ccttgctccc gagcgcaccc gcgcggcact cgcgacgggc gtccttctcg cagcgggttt
     9241 cggcgtgttt tcttcgagtg ctgaagaggg gggcttgcca gcaggcacgg ccggtgcggc
     9301 ggccgaggcg ccctcttcac cttctaagaa gatcttctct tccgcggttg cagacaagac
     9361 ctccgtctca atcggggatc gggtcgtcgt gacgtactcg gccaagctgc cggcgggcgc
     9421 gacgctctct ctggattcac tggtcacacc tgctcccgag gagggtgcga ggccgccggg
     9481 cggggccgtc ctcgagttcg aacttccctc cgaacctact ctcaagaaat ccggggattt
     9541 cttcgaatgg tcgcagtcgg tcgcgctgtt ccccttcgtg gcgggaaccg tgcaggtgcc
     9601 cgggcctcac tacacgttcg aagaatcttc ttccggccgc cgcctcgacg tgcgcccgcc
     9661 ctcggtggag ctgaccgtgg cgtcgcgtct cccgaaggat cagaagcccg agcagatcgc
     9721 cccgaaggcc gacaagggaa tccgcatccc cccgatcccc gcaaagttct gggcggctct
     9781 cggcgcggtc gtggcgctcg ccgccggcct cgtctggtgg ggaatcctgc gccgccggcg
     9841 ccagggcgac ctcggcgtgc ccgcctctcc gcccgtgccg cccggcgaag agctgcgcct
     9901 cgagctcgac cgcctcgctc ccgccgtcga cacgctcggc gacgacgcgc gcggcttcta
     9961 ctcggagctc acgcacgccg tgaagcgcta cctcgagcgg acgacgggcg accccgtcct
    10021 cgaatggacg acgttcgaga ccgtccgccg cttccgcgag aagggcttcg acctgccggc
    10081 cgacgtcgcg ctgcccgagc ttctcgcgtc ggccgaccgc gtcaagttcg ggaagggcgc
    10141 tgccacgcgg gacgacgcac gcgaggggct cgcccgcgcc cgccgcctgc acgaccacgt
    10201 cgaagcgcgc gaggcggcgc gccgcgcggc cgaagcggcg gccgctgcga gcgcgaagag
    10261 gaaggcgtcg tgacgatccc cgccgccctc cgcgacttcg tggccagcca cgtcccgccg
    10321 ggcctctcgt tcaaggaccc gctcttcctt ctcctgctcg tcctcgtccc ggtcgccatc
    10381 gccctgaaga tcctgcgcga gaggcgcggc gacggcgctc tcgtcgtctc gacgctcgcc
    10441 ccggtcgcgc gcgtcgtccc gtcgtggcgg gtccgcctgc gccacctgcc gttcgcgctc
    10501 gcgctcgtcg gctacgcggg actcgtcgtc tctctcgcgc gcccgcgcct cggcctcgaa
    10561 cgcacggagt cgtggaccga gggcgtcgac atcttcgtcg cgctcgacgc ctcgggctcg
    10621 atggcggccg aagacttcaa gccgaagaac cgcttccacg tcgcaaaggc ctgcacgcgg
    10681 gcgttcatcg agggtcgccc caacgaccga gtgggcctcc tggtcttcgc gggccgctcg
    10741 cgcaccgtga gtccgctcac caccgaccgc gtcatggtcc tcgaccgcct cggaggcctc
    10801 aagctcgggg accagggcga cggcaccgcg atcggactcg cgctcggcaa cgcgctcgcg
    10861 cgcctcaagc cctcgaaggc gaagtcgcgc gtgatcgtcc tcgtgacgga cggcggcaac
    10921 aacgcgggag agatcgaccc ggacaccgcc gccggcgtcg ccaaggcgct cggcgtgagg
    10981 gtctacaccg tcggcgtcgg gacgaacacc gggcccgtcg agatcccgat cccgatgaag
    11041 gaccccgaaa cgggccgggt ggtggaacgc cggatccggg cgaacgtgga cgtggacgag
    11101 gcgctcctgc agcgcatcgc gaaggagacg ggcgcccgct acttccgcgc gaccgactcg
    11161 cagggcctcg cggacacctt cgcgacgatc gacaggctcg agaagaccga gatcaagacg
    11221 acgcgttaca cgcgtttccg cgaggtcttc cccgaggtcg cgcggcccgc ggccctctgt
    11281 ctcgcgctct cggcgctcgg ggcgcttctc ctcttcccgg tggcgccccg atgaacgctc
    11341 tccgcggcct ccttgccgcc ttttcatcgc cgtacgcgct cctcgtgggc gcgggagcgg
    11401 cgctcgcctt cggcgtcctc ctcttctggg ccgccggccg ccgcgcggcc cgcgcggctt
    11461 ccctcgtctc gccggagctg gccgcgaaag cccgcctcgc gccgccc
//
LOCUS       DAOMFY010000112        11282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1782_length_11282_cov_7.317004, whole genome shotgun sequence.
ACCESSION   DAOMFY010000112 DAOMFY010000000
VERSION     DAOMFY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1782_length_11282_cov_7.317004"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLB01_10515"
     CDS             <1..411
                     /locus_tag="PLB01_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase 2"
                     /protein_id="HQQ77778.1"
                     /translation="LARPGLTHVFPSPAAVADADVARVGLPRARAAALRAFARAVADG
                     SFVLGAPKGLQDFEERLTALPGFGPWTAHVVALRALGEGDAFPDGDLGLVRAMERAGV
                     KRGRIAARAERWRPYRAYATLHLWASLADGKGGA"
     gene            408..932
                     /locus_tag="PLB01_10520"
     CDS             408..932
                     /locus_tag="PLB01_10520"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQQ77779.1"
                     /translation="MKREKEKEILRRVRVPTPAGAVVLVLRGSALAGLAFEDRWRGLS
                     VSLARRFGKFTFKENLQAETVAEALRRYFAGDLHALDPIEVDTGGTAFQESVWRALRR
                     IPAGTTWTYARLAREIGRPTATRAVAAANGANPVSIVIPCHRVIGSDGSLTGYGGGLP
                     RKRWLLVHEGALLL"
     gene            964..2313
                     /locus_tag="PLB01_10525"
     CDS             964..2313
                     /locus_tag="PLB01_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ77780.1"
                     /translation="MTPLLVENALVIGMTRPADVVPGGALFVEDGAIAARDAEAQTRA
                     RSYAAAGRPFQRLNAKGMWVLPGFVQTHVHLCQTLLRNGPDGLQLLPWLSTHVWPGEA
                     AHDEATLEVSARLGLAELLSGGTTSVLDMATAYHTDAVFRAAAAAGLRVTSGNALMDD
                     PGTNPAGLFRDTRAALAETERLAKAWHGGNGGRLRVAFCPRFAVSCTDRLLREVGERA
                     KAEGFLVHTHASENLDEIALVAQRTGRRNVRYLDDAGISGAHVVLAHVVHADADERTL
                     LASRGTTVAHCPSSNLKLGSGICPVPEYRAQGIRVTLGADGAPCNDRLDAFTEMRLAA
                     LLPKVRLGPAALPAWDVVRMATAEGAEALGLNAGTLETGRRADFVLLDPHSGFAGPVS
                     WREDPYGPIVYSMDRSHVAATYVDGEARYVRGEAFPLKPSAAEIDTAVAALKSRRRP"
     gene            2326..2793
                     /locus_tag="PLB01_10530"
     CDS             2326..2793
                     /locus_tag="PLB01_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQQ77781.1"
                     /translation="MAGKKSPPLLKTIVVGTDFSVCAARALSFAVSLASSQGAKVHVV
                     HVLVEPVQAFDVAGALPYLDVSTQKEWEDATKKRLAAAVASAEKRGVDASSEFLWGRP
                     SDAIIDSAKRAKASLVVLGTHGRNALEKLLIGSTAERVVRLCPVPVLTVREKR"
     gene            complement(2800..4380)
                     /locus_tag="PLB01_10535"
     CDS             complement(2800..4380)
                     /locus_tag="PLB01_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQQ77782.1"
                     /translation="MKNLFADRPIPNLMRGHEIGSDLALSADVCVVGSGPGGAIAAAR
                     LAASGARVILLEEGGFFTRKADFHMQEAEAYPKLYQDHGNRATDDLGITILQGRGVGG
                     GTLVNWTTCFRTPERTLALWRERHGLSDFTTERFGPHWDEVESRLSIRQVDVSEVNEN
                     NAVLLEGAGKLGIATELVRRNVMSCLHTGYCGMGCPTNAKRGMALTYLPDAGGKGARV
                     YADCRAVRLVLDARRERITAVEADVLDGMTDRPSGRRVTVMAARFVVAGGAINSPALL
                     LRSGLPDPHGRVGKRTFLHPVVATVALFDRRIDPFYGAPQSVASHAFVERPAKIGFFL
                     EVPPLHPMLASLALPGFGAAHRDGMKTLAKTNAIIALLRDGFLAEDEGGTVSLREGGR
                     RLSIRYSLGPAHAEAMREAMKVSARIQLAAGAREVRTLHKDPVVIREERDLRVIDERP
                     IGPNLLGVFTAHQMGGCAAGADPADSVVDGNLRHHQLANLWVFDGSVFPTSLGVNPQL
                     SILGIVSASAERMIASKT"
     gene            complement(4377..4913)
                     /locus_tag="PLB01_10540"
     CDS             complement(4377..4913)
                     /locus_tag="PLB01_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HQQ77783.1"
                     /translation="METRRGFLKKGILGGALLVVGGTAFLAFRRTLRAAKPRNPLLVL
                     SENEYAIFAAVAERVVAVDGDSPSTDAVDVAGRADAVMAQWPDPVQMEFRRVLHLFES
                     ALTGALTRTGYETFTASPARMQDARLAVWAGSRVAVFRSGYQAMKRLACACYYAAPES
                     WPATGYPGPPELPFADGE"
     gene            complement(4981..5559)
                     /locus_tag="PLB01_10545"
     CDS             complement(4981..5559)
                     /locus_tag="PLB01_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HQQ77784.1"
                     /translation="MTVPPTPVLPPSAIAEEYRTAVKSYGAKTPIQGVQVHRVPRFVD
                     DRGFFQEIYRAKADHPGSEALAEFFRDVPVAQINFTIVNAENHVKGLHYHLKQQDVWY
                     CPHPYKAKFVLFDVRKDSPTYGATQVLVAGDGQDLLVRIPEGVAHGYRALTNPCALFY
                     IVTRTFDVKDPDEWRIAWDHPAVKDLWDVPNG"
     gene            5641..7167
                     /locus_tag="PLB01_10550"
     CDS             5641..7167
                     /locus_tag="PLB01_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQQ77785.1"
                     /translation="MAQNSLFATKSVAQLLAEGSDEGEHSLKRALGRWSLVAIGIGAI
                     IGAGIFVMTGIGAHGSLNEDGTVKILGAGPALALSFVLAGIACVFAGLCYAEFASMIP
                     LAGSAYTYAYATLGELFAWIIGWDLVLEYMMGAATVSVGWSGYCVKFLKLFGIHLPEW
                     MTNDWITYSRGAAAALAGGHPWPAPPTIAGIPIVFNLLALGAVLLVTAVLARGIQESA
                     KFNAGIVATKTAVVVMVIIVGAFFVKAANWGTGWSDFAPNGFAGIASGAAYIFFAYIG
                     FDAISTTAQEAKNPQKDMPFGIIGSLAVCTVLYIAVALVLTGMVHWSKIDLKAPIAQA
                     FESYGLGWAVFLITGGALAGLTSVMLVMLLGQTRVFFAMSRDGLLPTKIFGEVHTRFR
                     TPFKMTWIVGIVCALLGAFTPIDDLGQMVNIGTLLAFVIVCAAVVILRRTNPGANRPF
                     RTPFVPAVPILGAVFCLYLMLNLGWHTWARLVIWLAIGLVIFFAYSRKNSHLTAGGTN
                     "
     gene            7169..7483
                     /locus_tag="PLB01_10555"
     CDS             7169..7483
                     /locus_tag="PLB01_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77786.1"
                     /translation="MKRAALLVLLVGAPLVLALFPALRRRIAHKARFLLLLWSGAVLF
                     FGLIGGPGRKSFLESEWGWDAGTLWPLFGAALFAAAFVSVFRDFRLSSPSKKSSSSSS
                     SS"
     gene            complement(7490..9808)
                     /locus_tag="PLB01_10560"
     CDS             complement(7490..9808)
                     /locus_tag="PLB01_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77787.1"
                     /translation="MRGRIGRAGERRRRPPQVLVWIILGGVAASRFFAIAASPGEIDE
                     AVFAGAVTHFDLFDLSPQAPGFPVWILLGRAFLPFCVTPFNALATASTILAAAGFFAL
                     WTWGCRTVGGWAALSGVVFAAALPVVWVNGGRAFSDTPATAFFLGALACLALADERRS
                     PNQTRWREMIAARRARLLCIAAGLLAAAGAGVRPHLVLAFGPALLLGIGRLAQRWDRR
                     DAVLSFLGAGAAGTAAWAAWLFAQAGGWAGLVASLSERAGFRAHAFATGTFGTFADSF
                     LVRDFLSPRRAAAVFLLAAIGLVALLLRRRRGVVDLLVILVPTFLSLWFLHSRAMSRY
                     SVPFAVVLALAVGAGLEAVLRKGPLAFGAAVLGAGLYAREAWPDVVASAHEETPPIAA
                     IRALERWVHPGRETIVADEDFHAFLRTERWEGRLVAWGYTDTEIASAPRQTNRRLVRL
                     ADFTAEPEPPDRRDPLWKTWLRGGRVAEMLGNRRLLVVAVRDPAPPLFGPGFGPKESA
                     PGKHSFRWAGPSARLLVAQDQGPLSATLSGERPDAGGPTTLTVSEAGTGRILLSRVIE
                     PGPFDFAILDRPVYGPMPGPAEYVITCDRTVDLPPSPGTSRPAKGCFTFLESTFSRPP
                     EHAWMRQGARVVADLGAPDDGRYDPEGFWQREKIADTGLDLRWTKGSASLAWAPQPGF
                     TPARVVLRARAVAAPKDAAIYVNGVFAAAVRVGSGLAEVSAPLAPAAAGFLSGPECAR
                     IEIHAATEVPKALGKGDDVRALGVAVDRIALE"
     gene            9885..>11282
                     /gene="pcnB"
                     /locus_tag="PLB01_10565"
     CDS             9885..>11282
                     /gene="pcnB"
                     /locus_tag="PLB01_10565"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HQQ77788.1"
                     /translation="MTVPRILPRAEHPISRGYIDPDALRVLYRLHRAGFKAYLVGGSV
                     RDLMTGRTPKDFDIGTDAKPQEVRRLFRNSRVIGRRFRLVHILFEGGKLVEVSTFRKT
                     PDAVQGEQGQEGDEDLLIRSDNTFGTPEEDAVRRDFTINGLFYDIATYAVLDYVGGVD
                     DLAARVVRTIGDPRLRFREDPVRMLRACEFAARLSFEMAPDLRTAIDELRREVTKSAA
                     PRVTEELLDPLRRGWGHDTYRLWSETGLFGSLLPEMDVEAGVRAPEGPVKDGFWAMLR
                     EADARTGGGERLDDAALLGMLFLPVVLAAVRRRGRPGTSAGPEQILLVLEDAVNPLAI
                     RMSLPNHTTHTLKQALYTMGRLTSTRPAEPAARRIVGRPYFPAAVTLLSLYAKATNRY
                     REAAAAWEEVVTRGSGTRVLQGTPFPDALVPPPDPGNVQPLRHHVGTRVDVGYQGSCV
                     SGRLDDLRAAAQVLAG"
BASE COUNT         1640 a         4074 c         3926 g         1642 t
ORIGIN      
        1 ctcgcgcgcc ccgggctcac gcacgtcttt ccgtcccccg ccgccgtcgc ggacgcggac
       61 gtcgcgcgtg tcgggctccc ccgcgcccgt gccgcggccc tcagggcctt cgcccgcgcc
      121 gtcgcggacg gctcgttcgt cctcggggct ccgaaaggcc tccaggactt cgaggagcgc
      181 ctcacggcgc tgcccgggtt cgggccgtgg acggcgcacg tcgtcgccct gcgagcgctc
      241 ggcgagggcg acgcctttcc ggacggcgac ctcggtctcg tcagggcgat ggagcgcgcg
      301 ggcgtgaaga gaggccggat cgccgcgcgc gccgagcgct ggcggcccta ccgcgcgtac
      361 gcgacgctcc acctctgggc gtcgctcgcg gacgggaaag gcggcgcatg aagagggaga
      421 aagagaaaga gattcttaga agggtaagag tgccgacacc cgcgggtgcg gtcgtgctcg
      481 tcctgcgcgg cagtgcgctc gccggcctgg ccttcgagga ccgctggagg ggattgagcg
      541 tctcgctcgc acggaggttc ggcaaattca ccttcaaaga aaatttgcag gccgaaactg
      601 tcgccgaggc gctgaggcgt tacttcgccg gcgacctcca cgctctcgat cccatagagg
      661 tcgacacggg cggcacggcc tttcaggagt ccgtctggcg ggctctcagg cgcatccccg
      721 ccggcacgac atggacgtac gcccgtctcg cgcgcgagat cggccgaccg accgcgacgc
      781 gcgccgtggc ggccgcgaac ggggcgaacc ccgtctcgat cgtgatcccc tgccaccgcg
      841 tcatcgggtc ggacgggagc ctcacgggct acggcggcgg cctcccccgc aagcggtggc
      901 tgctcgttca cgaaggagcc ttgctgctct gaaccgtccg ccattggata atggcgggct
      961 gccatgacgc ccctcctcgt cgagaacgcc ctcgtgatcg ggatgacgcg ccccgcggac
     1021 gtcgtcccgg gcggcgcgct gttcgtcgag gacggcgcca tcgccgcgcg cgacgccgag
     1081 gcccagacgc gcgcacgctc ttacgcggcc gcgggacggc ccttccagcg gctgaacgcg
     1141 aagggaatgt gggtcctgcc cggcttcgtc cagacgcacg tccacctctg ccagacgctc
     1201 ctgcgcaacg gccccgacgg cctccagctg ctcccgtggc tctcgacgca cgtctggccg
     1261 ggcgaggccg cgcacgacga ggcgacgctg gaggtctcgg cgcggctcgg ccttgccgag
     1321 ctcctctccg gcgggacgac gtccgtcctc gacatggcga cggcgtacca cacggacgcg
     1381 gtcttccgcg ccgcggcggc cgcgggcctt cgcgtcacga gcggcaacgc cctcatggac
     1441 gatcccggga cgaatccggc cggcctcttt cgcgacacgc gggcagccct ggcggagacc
     1501 gagcgcctcg caaaggcctg gcacggcgga aacggcggcc ggctgcgcgt cgcgttctgt
     1561 ccccggttcg ccgtctcgtg cacggaccgc ctcctgcgcg aggtcggcga gcgcgcgaag
     1621 gcggagggct tcctcgtcca cacgcacgcc tccgagaacc tcgacgagat cgccctcgtc
     1681 gcccagcgca cgggacggcg gaacgtccgc tacctcgacg acgccggcat ttccggcgcg
     1741 cacgtcgtgc tcgcgcacgt cgtccacgcc gacgccgacg agcggacgct cctcgcctcg
     1801 cgcggcacga ccgtcgcgca ctgcccgtcg tcgaacctca agctgggctc cggcatctgc
     1861 cccgtgcccg agtaccgcgc gcagggcatc cgcgtcacgc tcggtgccga cggcgctccc
     1921 tgcaacgacc ggctcgacgc cttcacggag atgcgcctcg cagccctcct tcccaaggtg
     1981 cggctcgggc ccgcggcgct cccggcgtgg gacgtcgtgc ggatggcgac ggccgagggc
     2041 gccgaggcgc tcggcctgaa cgcggggacg ctcgagacgg gccgccgggc cgacttcgtg
     2101 ctcctcgacc cgcactcggg cttcgccggc cccgtctcgt ggcgcgagga cccctacggg
     2161 cctatcgtgt acagcatgga ccggtcccac gtcgccgcca cctacgtcga cggcgaggcc
     2221 cggtacgtcc gcggggaggc cttcccgctg aagccctccg ccgccgagat cgacaccgcc
     2281 gtcgccgcgc tcaagtcgcg ccggcggccc tgagggaggc acgccatggc cggaaagaag
     2341 tccccgccgc ttctcaagac gatcgtcgtc ggcaccgact tttccgtgtg cgcggcgcgc
     2401 gccctctcgt tcgcggtctc gctcgcgtct tcgcagggcg cgaaggtcca cgtcgtgcac
     2461 gtcctcgtcg agcccgtgca ggctttcgac gtcgccggcg ccctccccta cctcgacgtc
     2521 tcgacgcaga aggagtggga ggacgcgacg aagaagcgcc tcgccgccgc ggtcgcctcg
     2581 gccgagaagc gcggggtcga cgcctcgagc gagtttctct ggggccgacc ctccgacgcg
     2641 atcatcgact ccgcgaagag ggcgaaggcc tcgctcgtcg tcctcggaac gcacgggcgc
     2701 aacgcgctcg agaagctgct gatcgggtcg acggccgagc gcgtcgtccg cctctgcccg
     2761 gtccccgtcc tcacggtgcg cgagaagcgc tgacccgcgt caggtcttcg acgcgatcat
     2821 ccgctcggcc gacgccgaga cgatgccgag gatcgagagc tggggattga ctccgagcga
     2881 cgtcgggaag acggagccgt cgaagaccca gaggttcgcg agctggtggt ggcggaggtt
     2941 tccgtccacg acggagtccg ccggatccgc ccccgccgcg cagccgccca tctggtgcgc
     3001 cgtgaagacg ccgaggaggt tcgggccgat gggccgctcg tcgatgacac ggaggtcgcg
     3061 ctcctcccgg atcacgacag gatccttgtg cagcgtcctc acctcgcgcg cgcccgcggc
     3121 gagctggatg cgcgccgaga ccttcatcgc ctcgcgcatc gcctcggcgt gagcggggcc
     3181 gagcgagtag cggatcgaca ggcgccttcc gccttcgcgg agcgacacgg tcccgccctc
     3241 gtcctccgcg agaaacccgt cgcgcaggag cgcgatgatc gcgttcgtct tcgcgagcgt
     3301 cttcatcccg tcgcggtggg ccgcgccgaa ccccgggagc gcgagcgacg cgagcatcgg
     3361 gtggagcggc ggcacctcga ggaagaagcc gatcttcgcc gggcgctcga cgaaggcgtg
     3421 agaggcgacg gactggggag cgccgtagaa ggggtcgatg cgccggtcga agagcgcgac
     3481 ggtcgcgacg acgggatgga ggaacgtgcg tttgccgacg cggccgtgcg ggtccggcag
     3541 gcccgagcgc aggaggagcg ccggcgagtt gatcgcgccg cccgccacga cgaaccgcgc
     3601 cgccatgacc gtcacgcggc ggcccgaggg ccggtccgtc atgccgtcga ggacgtcggc
     3661 ctcgacggcc gtgatgcgct cgcggcgcgc gtcgaggaca aggcgcacgg cgcggcagtc
     3721 ggcgtacacg cgcgcgccct tgccgcccgc gtcgggcagg tacgtgagcg ccatcccccg
     3781 cttcgcgttc gtcgggcaac ccatcccgca gtagcccgtg tggaggcacg acatgacgtt
     3841 gcgccgaacg agctccgtcg cgatcccgag ctttcccgcg ccctcgagga gaacggcgtt
     3901 gttttcgttc acctccgaga cgtcgacctg ccggatggag agacggctct cgacctcgtc
     3961 ccagtgcggg ccgaatcgct ccgtggtgaa gtcggaaagc ccgtggcgct cgcgccagag
     4021 ggcgagcgtc cgctccggag tccggaagca cgtcgtccag ttcacgaggg tgccgccgcc
     4081 gacgccacgc ccctggagga tcgtgatccc aaggtcgtcc gtcgcgcggt tgccgtggtc
     4141 ctggtagagc ttcgggtagg cctcggcctc ctgcatgtgg aagtcggctt tcctcgtgaa
     4201 gaagcccccc tcctcgagga ggatcacgcg cgccccggac gccgcgaggc gtgcggcggc
     4261 gatcgcgccg cccggaccgg agcccacgac gcagacgtcg gccgagagcg cgaggtccga
     4321 gccgatctca tggccgcgca tgaggttcgg gatcgggcgg tccgcgaaga ggttcttcat
     4381 tccccatccg cgaagggcag ctccgggggc cccgggtacc ccgtcgcggg ccagctctcg
     4441 ggcgccgcgt agtagcacgc gcatgcgagc cgcttcatcg cctggtagcc ggatcggaag
     4501 accgcgacgc gcgatccggc ccaaaccgcg agacgcgcgt cctgcatgcg cgcgggcgac
     4561 gccgtgaacg tctcgtaacc ggtccgcgtc aacgctcccg tcagggcgct ctcgaagagg
     4621 tgaaggacgc gccggaactc catctggacg gggtccggcc actgcgccat caccgcgtcc
     4681 gcgcggcccg cgacgtccac ggcgtccgtc gacggcgaat ccccgtccac cgcgaccacg
     4741 cgttcggcga cggccgcgaa aatcgcatac tcgttctcgg aaaggacgag aagagggttg
     4801 cgcggcttcg ccgcgcgaag agtccttcga aaggcgagga aggccgtgcc tccgacaacc
     4861 agcagcgcgc caccgaggat gcccttcttg agaaatcccc gcctcgtctc caccggtttc
     4921 tctaccttct aagaaatccc ccggccggcg cggagcgccg gctgcagaat ctcccggtct
     4981 tcacccgttc gggacgtccc agaggtcctt taccgcgggg tggtcccacg cgatgcgcca
     5041 ttcgtccgga tccttgacgt cgaacgtgcg cgtgacgatg tagaagagcg cacacgggtt
     5101 cgtgagggcg cgatagccgt gcgcgacgcc ctcggggatg cggacgagaa ggtcctgtcc
     5161 gtcccccgcg acgaggacct gggtcgcgcc gtacgtcggc gagtccttcc ggacgtcgaa
     5221 gaggacgaat ttcgccttgt acgggtgcgg gcagtaccac acgtcctgct gcttgaggtg
     5281 gtaatgcagg cccttgacgt ggttttcggc gttcacgatc gtgaagttga tctgggcgac
     5341 gggaacgtcc cggaagaact cggcgagcgc ctcgctcccc gggtggtcgg ccttcgcgcg
     5401 gtagatctcc tggaagaagc cccggtcgtc cacgaagcgc ggcacacggt ggacctggac
     5461 gccctggatc ggggtcttcg ctccgtagga cttgaccgcc gtgcggtatt cctcggcgat
     5521 tgcggacggc ggcagcacgg gtgtcggcgg aacggtcatg cggatcctct gagacggatt
     5581 ctatggtgtt atcgtccggg cgttccgtac gactcgagat cgggaagagg aggtcgacac
     5641 gtggcccaga attccctgtt cgcgacgaag tccgtcgcgc agctgctcgc ggaaggctcc
     5701 gacgagggcg agcacagcct caaacgcgcg ctcgggcgct ggtcgctcgt cgcgatcggc
     5761 atcggggcca tcatcggcgc cggcatcttc gtcatgacgg gcatcggcgc ccacggctcc
     5821 ctgaacgaag acggcaccgt aaagatcctt ggcgcggggc ccgcgctcgc cctgtcgttc
     5881 gtcctcgcgg gaatcgcgtg cgtcttcgcg gggctctgct acgcggagtt cgcctcgatg
     5941 atcccgctcg cgggctcggc gtacacctac gcgtacgcga cgctcggcga gctctttgcc
     6001 tggatcatcg gctgggacct cgtcctcgaa tacatgatgg gcgccgcaac cgtgtcggtc
     6061 ggatggtccg gctattgcgt gaaattcctg aagttgttcg ggatccacct gccggagtgg
     6121 atgacgaacg actggatcac gtacagccgc ggcgctgcgg ccgcgctcgc gggcgggcat
     6181 ccctggccgg ctccgccgac gatcgcgggc atcccgatcg tcttcaacct cctcgcgctc
     6241 ggcgcggtcc tgctcgtgac ggccgtcctc gcacgcggca tccaggagtc cgcgaagttc
     6301 aacgccggca tcgtcgccac gaagaccgcc gtcgtcgtga tggtcatcat cgtgggcgcc
     6361 ttcttcgtga aggccgcgaa ctggggaacg ggctggtccg acttcgcgcc gaacggcttt
     6421 gcgggcatcg cctcgggggc cgcgtacatc ttcttcgcgt acatcggctt cgacgcgatc
     6481 tcgacgacgg cgcaggaggc gaagaacccg cagaaggaca tgcccttcgg gatcatcggc
     6541 tcgctggccg tctgcacggt cctctacatc gcggtcgcgc tcgtcctgac gggcatggtc
     6601 cactggtcga agatcgacct caaggcgccg atcgcgcagg ccttcgagag ctacggcctc
     6661 ggctgggcgg tcttcctcat cacgggcggc gcgctcgccg gcctgacgtc cgtcatgctc
     6721 gtcatgctcc tcggccagac gcgcgtcttc ttcgcgatgt cgcgcgacgg cctgctcccg
     6781 acgaagatct tcggcgaggt ccacacgcgg ttccggacgc ccttcaagat gacgtggatc
     6841 gtcggcatcg tctgcgccct gctcggcgcc ttcacgccga tcgacgacct cgggcagatg
     6901 gtcaacatcg gaacgctcct cgcgttcgtg atcgtctgcg cggccgtcgt catcctccgg
     6961 cgcacgaacc ccggcgcgaa ccgcccgttc cggacgccgt ttgtgccggc cgtcccgatc
     7021 ctcggagccg tcttctgcct ctacctcatg ctgaacctcg gctggcacac ctgggcccgc
     7081 ctcgtgatct ggctcgcgat cggcctcgtg atcttcttcg cctacagccg caagaactcg
     7141 catctgacgg ccggaggcac gaactgacgt gaagcgcgcg gcgctcctgg tcctcctggt
     7201 cggagcgccg ctcgtcctcg ccctcttccc ggctctccgc cgccgcatcg cgcacaaggc
     7261 gcgcttcctg ctgctcctgt ggtcgggggc ggtgctgttc ttcgggctga tcgggggacc
     7321 ggggagaaag agcttcttag aaagtgaatg gggctgggac gcgggcacgc tctggccgct
     7381 gtttggcgcg gcgttgttcg ccgccgcgtt cgtgtccgtg ttccgagact tccgcctctc
     7441 ttcaccttct aagaaatctt cgtcttcgtc ttcctcttcc tgatctctcc tactcgagcg
     7501 cgatgcggtc gaccgcgacc ccgagggcgc ggacgtcgtc gcccttgccg agcgccttcg
     7561 ggacttccgt ggccgcgtgg atctcgatcc gcgcgcactc cgggccggag aggaaccctg
     7621 ccgcggccgg cgcgagcggc gccgagacct cggcgaggcc cgaccccaca cgcaccgcgg
     7681 ccgcgaacac cccgttgacg taaatcgcgg cgtccttcgg cgccgcgacc gcgcgcgcgc
     7741 gcagcacgac ccgcgccggc gtgaagccgg gctgcggcgc ccacgcaagg gacgcagacc
     7801 ctttcgtcca gcggaggtcg aggccggtgt ccgcgatctt ctcccgctgc cagaaaccct
     7861 ccgggtcgta gcggccgtcg tccggcgccc cgagatccgc cacgacgcgg gccccctgcc
     7921 gcatccacgc gtgctcgggg ggacgcgaga acgtggactc gagaaacgtg aagcagccct
     7981 tcgcggggcg cgacgtgccg ggcgagggcg gaaggtccac ggtgcggtcg cacgtgatca
     8041 cgtattccgc gggccctggc atcggaccgt agacggggcg gtcgagaatc gcaaagtcga
     8101 acggaccggg ctcgatcacg cgcgacagga ggatcctccc cgtgcccgcc tccgagacgg
     8161 tgagggtcgt cggtccgccc gcgtccggcc tctcgcccga cagagtcgcg gagagcgggc
     8221 cctggtcctg tgcgacgagg aggcgcgcgg agggccccgc ccaccggaac gaatgcttcc
     8281 cgggcgcgct ctccttcggc ccgaatcccg ggccgaagag cggcggcgca gggtcgcgca
     8341 ccgcgacgac gaggaggcgc ctgttgccga gcatctcggc gacgcgtcct ccgcgcagcc
     8401 aggtcttcca gagcgggtct cgacggtccg gcggctcggg ctcggccgtg aagtccgcga
     8461 gacggaccag gcgcctgttc gtctggcgtg gcgccgaggc gatctccgtg tcggtgtacc
     8521 cccacgcgac gagccggcct tcccagcgct cggtcctgag gaaggcatga aaatcctcgt
     8581 ccgcgacgat cgtctcgcgg cccggatgga cccaccgctc gagcgcccgg atcgcggcga
     8641 tcggcggggt ctcctcgtgc gcgctcgcca cgacgtcggg ccacgcttcc cgggcgtaga
     8701 ggcccgcacc gagcaccgcc gcgccgaagg cgagcggccc ctttctcaga acggcctcga
     8761 ggcccgcacc cacggcgagc gcgagcacga cggcgaacgg gaccgagtag cgcgacatgg
     8821 cgcggctgtg caggaaccag agcgacagga acgtcggaac gaggatcacg aggaggtcca
     8881 ccacgccccg gcgccgcctg aggagcagcg cgacgagccc gattgccgcg agaaggaaca
     8941 cggcggccgc gcgtctcggc gagaggaagt cgcgcacgag aaacgagtcc gcgaacgttc
     9001 cgaacgtgcc ggttgcgaag gcgtgcgcgc ggaagcctgc gcgctcggaa agggacgcca
     9061 cgaggccggc ccagccgccc gcctgcgcga agagccaggc ggcccaggcg gccgtcccgg
     9121 ccgcgccggc gccgaggaaa gagagcaccg cgtcgcggcg gtcccagcgt tgggcaagac
     9181 ggccgatccc gagaagcagc gccggcccga acgcgaggac gaggtgcggc cgcacgcccg
     9241 ccccggcggc ggcgaggagg cccgcggcga tgcacaggag gcgcgcacgg cgtgcggcga
     9301 tcatctcgcg ccagcgcgtc tggttcgggg atcggcgctc gtcggcgagc gcgaggcacg
     9361 cgagagcgcc gaggaagaag gccgtggccg gggtgtccga gaacgcgcgg ccgccgttca
     9421 cccagacgac gggaagcgcc gccgcgaaga cgacgcccga cagcgccgcc cacccgccga
     9481 ccgtgcggca gccccaggtc cagagcgcga agaatcccgc ggctgcgagg atcgtcgacg
     9541 ccgtcgcgag ggcgttgaac ggcgtcacgc agaaaggcag gaacgcacgt ccgagaagga
     9601 tccacaccgg gaacccgggc gcctgcggcg acaggtcgaa caggtcgaag tgcgtcacgg
     9661 cgcccgcgaa gacggcttcg tcgatctcac ccggcgaggc ggcgatcgcg aagaagcgcg
     9721 aggcggcgac gccgccgagg atgatccaca cgagtacctg cggtggccgc cgccgccgct
     9781 cgccggcccg cccgatccga cctctcacgc cgaggagtgt tccatttttc ggcccgcttc
     9841 gaaaccgcta caatgcaggg gatgtcgttc cggcagcccg cgggatgacc gttccccgca
     9901 tcctccctcg cgccgagcac cccatttcgc gcggatacat cgatcccgac gcccttcggg
     9961 tgctctaccg gctgcatcgc gccggcttca aggcgtacct cgtgggcgga tcggttcgcg
    10021 acctcatgac gggccgtacg ccgaaggact tcgacatcgg cacggacgcg aagccgcagg
    10081 aagtccggcg cctcttccgg aactcgcgcg tgatcgggag gcggttccgc ctcgtccaca
    10141 tcctcttcga gggaggcaag ctcgtcgagg tttccacgtt ccggaagacg cccgacgccg
    10201 tccagggcga acaggggcag gagggcgacg aagacctcct gatccgctcc gacaacacgt
    10261 tcgggacgcc cgaggaggac gcggtccgcc gcgacttcac gatcaacggg ctcttctacg
    10321 acatcgcgac ctacgccgtc ctcgactacg tcggcggagt cgacgacctc gccgcccgcg
    10381 tcgtccgaac gatcggcgat ccgcgcctcc ggttccgcga ggaccccgtg cggatgctcc
    10441 gggcgtgcga gttcgcggcg cgcctctctt tcgagatggc tcccgacctg cgcaccgcca
    10501 tcgacgagct ccgccgcgag gtgacgaaga gcgcggctcc gcgcgtcacc gaggagctcc
    10561 tcgacccgct tcggcgcggc tgggggcacg acacgtaccg cctctggtcg gaaaccggac
    10621 tcttcggctc gctccttccc gagatggacg tcgaggcggg ggtgcgcgcg cccgaaggcc
    10681 cggtgaagga cggattctgg gcgatgctcc gggaggccga cgcgcgcacc ggcggcggcg
    10741 aacgactcga cgacgcggcg ctcctcggca tgctcttcct ccccgtcgtg ctcgcggccg
    10801 tcaggcgccg cgggcgcccg ggcacgagcg ccgggcccga gcagatcctc ctcgtcctcg
    10861 aggacgccgt gaacccgctc gcgatccgga tgtcgctccc gaaccacacg acgcacaccc
    10921 tcaagcaggc gctctacacg atggggcggc tcacgtcgac gcggccggcc gagcccgccg
    10981 cgcgccgcat cgtgggccgc ccgtactttc ccgcggcggt gacgctgctc tccctctacg
    11041 cgaaggcgac gaaccgctac cgcgaggccg ccgctgcgtg ggaagaggtc gtcacacggg
    11101 gatccgggac ccgcgtcctg caggggaccc cgttcccgga cgccctcgtg ccgccgcccg
    11161 accccggcaa cgtgcagccg cttcgccatc acgttggcac gcgtgtggac gtcggctacc
    11221 agggctcctg cgtgtcgggg cggctcgacg atctgcgcgc ggcggcgcag gtgctggcgg
    11281 gc
//
LOCUS       DAOMFY010000113        11173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1799_length_11173_cov_8.747437, whole genome shotgun sequence.
ACCESSION   DAOMFY010000113 DAOMFY010000000
VERSION     DAOMFY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1799_length_11173_cov_8.747437"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>11173
                     /locus_tag="PLB01_10570"
     CDS             <1..>11173
                     /locus_tag="PLB01_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ77789.1"
                     /translation="AGSGATPDRARVTLAGPGSVAIVAPDANPATRPPVLPVNLDAPL
                     VGVDLPSPVPTLTATIALDPPVVSPTGRATARVVARSSDGTTPWPSGLAVQGYLDEKL
                     ILSGGAGELYEAPFTADLVLYHPPLSAADLGGTVPGAAGALTFRVSPSPRAAQVLLDT
                     GYENVRLFPFTDQLERGQVLGPAGGSVTTADGVELVVPESALAQKTVVSARKLTASEL
                     ALLPPVAGYTTVAGVRVSLSGATLARAATLKLNAPSGLPADAADDPRYVLAVFVEMPD
                     DGRGAFANAVSRVTLLPAAGAVPPKLVAAPEASGSPLPFPGVAGEGVFLYLHANAPVG
                     FVTGRVTSPNGAGIAGSRVTAAGLGTANLSVPGGVYAVPAPAGAPTLNALHPTLGVAG
                     SGQVPSLAKGQVATLDLVLTPVPPTIRALQPASGAVDQPVGSTVSVAFSAALDPSSVT
                     ASTLSVSLADATTGAPTGLLFNGAISLSADRTTVVFTPARPLPPGKRIAARFTGGVRD
                     AAGTLYGGTLPVDWSFTTSTQFVTGGAIHPEKIRLLVPVNGVAQIVGADGALPLVTGG
                     TPPWSVWADVEGPVCPSRVTQPANANGGFTLNAGCPPTVSVTIASKVWLHVIDPTGAE
                     AATFRLGPFATPDGKGFVAAPGDETVFTTAEGVEVTAPAGAFDVPTLVKVSKQPLDSL
                     NVALNPGLEMGAVLNVDFDGTAKETLRLRIPVTTASPVGGLVFAGTPIDLPWGRKLKI
                     LDVGRLVDDGKGGKLISTLEADQPDDPSLTAGAAPSSATSGGRTALVSRKLLRTIAAE
                     MTARGSVAFVFGTTLPLMGVTGAVVSGTLFVLYNALADAFVFQALANDWSGRYVLPAY
                     PGQALSVIKRDTATGWILARQDYANPAGSSGLTNLGTLDAGPDTPPVLVDASPIRVLR
                     FTAPPVDAARGIDTLRLALEMEARVGSDRTASLASVPGFAFADGTSVDFLDFTQASAK
                     TPSGAVSARSFVSGGAFATGPVAAQPADDMFVYVSPGELDPAGVSELTLTFSRRIDNP
                     TGNAADLVALWDCGDVARVLSGSAPSCPGASVKVAATADLALAGAQIVVHLPGRLAEG
                     RQYRLDLVGVKSAPKGIAYPPGAPSSFFFATRRTGDPFGKIVTSALGDTNSARDLLKF
                     GHLLMVGSATGRLVAVDVSDPKNPTTWASLVNVDATGTATDAKADMLRAFATDGHGRL
                     FYNVQFGSSWAVRTVRIEDIREHAAPCPATPFFPGVPCFTSIEGGARTAFAPGAIGTL
                     LGPDYLALVGSLPTGTPSSMEVVVRDESEPPANGSYELKELYERPEQHGADSFKSLAA
                     VNGFYEFDVKVFTETHLANRPSSCGETPWDRYQRVSVDNLSTGQTWSKDVENLGPRGS
                     VNEKIRIRGRLGDRLRIRYNVLALGYLSIVGSGVSVLDLNRMYRNPGPVAAQANKSQC
                     DRRIGKYEGADVALYSVCPAPNSAGAAQAAPALNGLSNTPALVAVPGPANVDVHSVLT
                     HFGVVHESAKLTTPGDLSIPSKAPLQSWQTPDGATCLGDRAPAGLRPSYRAIAFARDA
                     AWSDLGIRAGSGAAFIGPALKPIRTTGDLLLVSMGSAGILSISLGPLANGDPIGRYWV
                     EGHSVYRLQADVSRGLLFAGGQDRNGNSIVDVWDLARANGGPTPDGVFPPISGQAVDP
                     RLLATLYVPWDTNHIGLDETGAGLVYTWGYRRAPDGSGGTVTEEGAFVMPFDAPSITF
                     AGVFRKDTAAPAAGTLAPSVRQLVTKLDPLGVPLRLSAFDERNTPADETCSDLDRALG
                     ANCRTAAFKVRVALPGSAAGRALGATLNAKVQSLRGLPEKRLLAQEDLGALVPPCGGT
                     EPCDGGAHAAQSGWPDRDVWVTLRRIGKGYSGADITSPDGPTSNAYDLYESDETVVLV
                     ADPRARFAYWKPYQALEVKSGNPPDERHQCRRCDRPTTLPQDASDTSVVELLAGGPYV
                     RAFLSPKPGSPDELATRAAILYFNGTNYPPPSGYVRVSGFADEVPSVTQIALAEPAQN
                     PAVWTAEAGASVRLTSGEGVVETRDFSAAARSLGFALDRTYRSGVAGYGPLGAAGWNA
                     SLFAHLRETALIPPVPGGSSSYVDYHDGAGQVWRFVGLPADVSCPAGTARDGGETYCT
                     PKGLYMRLDKLTDGTFRLVGRNNDTLRFDPAGRLTMISDRHAQGDPDKKGSSVRLYYT
                     PAGDLVRVQDEMGRVYRLTYFDSTDPANAGLLKKIEDFSSPARTVEFEYDRRRLSKVT
                     LPKVTVVPSKLGIVPGIPVISYGYGASTNANTAPPLHGPLWSGKLETFTLPGETTERI
                     RFTYDPTSGRASPVLAGKATTVTWKLTATPATAEPAKSVEVLDPSGLRTTYTLDDKGH
                     VANVLRKGVEVSKGGAPALTDLTESYLYRDDGRISFATRADGTKMDYAPPPAGPQRLA
                     YRIPSVTEIGKDPDGSIKRGFEYQGDGLVEKITDPEGRTITPGVPAAGLKVDSKTSDG
                     LSSSTTFDDFGRPKTYGGGAGTKGETTYRDDALGVKGGGFPLEVVKGDKPSGKYVGAE
                     FERDERGNPTEIRESSGAVTKIDYDEWDRPVHETFGRGGEQEAPGEVFRGFDTAGRLK
                     KEIRTQLNAGDVTTTWEYNDRDQVTEIKRDHVASATPFAAPDATSTTKFTYDVTSGFL
                     KSVSTDQGTTTTYRFERAGRLAAVTPDGSGTRQFGYDELGRPTYVFDGDKGSWTGAYD
                     SFGRLGTENLPGGGIVTRTFDKAGGLKTAKLADGLGAVLSEIPFAKVTSWGALTNLER
                     RVTAGTSLLMTRELDAEGRLTLQMSDAHKDLEMRFEAGTGRPAFVGDAVNELTLGYGS
                     AATAWPKSLTYGVAVDPSKAAVPTVISTLKHDTLGRVVEDLRDDGTLRTARFDEEGRP
                     VETTDGASATSLRWDSRGALVQLIRPAGQGATGLGYDHDGRLTAKKTDAGGTSYVSMY
                     DYYAPGESHAGLLRTFARTDGPTLTVKEYYPDDMAKTVKLSDGATVGLEYDDANRMTK
                     TTVTGGSSVPGGVLYAWDAASRMRGASRLMPNGIDVDAAAAVVPGAYDFAGRPHTETV
                     GSRPALTRDYDLLGGVTSLGLPLGVGAARPFSYTRVFEPLTHRLASVTGAGDAPPPGG
                     VAPALLGASWAWVGDDRVLGVTSNGPLHAAHRFGYVGGPGGPDGSVPGAAKWKLGTLT
                     VGTDPGAKNLLPFGDPLAPGAAPWGQFAYGYNSSITGDGTKLGRRVTGVGLAGLLAGS
                     LLSNLGYAYGLDASKRLTTAYAGPGSVTGADAAAAIAAFERFKYEYGSADQLQKETRE
                     TSGRSVGYETGGDGRPDSRQLDAGPKSLFLYDALKRRTGDDRFTFTWHYDGKIATAVV
                     KDCWPIENGESQAPGCDSGYTRPAQAGHKLVFAYDALSRLLTRTHLGEIPSGGGEGDR
                     PFIETREYLFDGNTLLAEVAKAKDGAIRWRKTYVPGADLHDHVQVRVETYDAAQNLVS
                     DRLYAYIRDEQGTVLALADEASDPTHPSIPIRYLYTPYGNAHAETGPELRKGSYGFGI
                     TSVKDASGATKTQTATGTLPGGIRLSFGIDVDLSTLATGVAFEKRATDGTWGALTEMA
                     IGRAPDAANSDVDILPLGGFAFATSYRVRTTAALKDVLGRTLADPKVLELPQTPPVIA
                     PGDIGASSYEYVRTFPVEYDSLEAAANDANGLFPGGQPMGFQGQYTDPLLGFLLTPNR
                     VYDARNGVWLSQDPLGDKDSPNLYGF"
BASE COUNT         1660 a         4157 c         3820 g         1536 t
ORIGIN      
        1 gcgggcagcg gcgcgacgcc cgatcgcgcc cgcgtcacgc tcgccggccc cggctccgtc
       61 gcgatcgtcg cgccggacgc gaaccccgcg acgcggccgc cggtcctgcc cgtcaacctc
      121 gacgctccgc tcgtcggcgt cgatctaccg tctccggttc cgacgctcac ggccacgatc
      181 gctctcgacc cgcccgtcgt ctcgcccacg ggccgcgcca cggcgcgcgt cgtcgcgcgg
      241 tcgtccgacg gcacgacgcc gtggccgagc ggcctcgccg tgcagggcta cctcgacgag
      301 aagctcatcc tctcgggcgg cgcgggcgaa ctctacgagg cgccgttcac cgccgacctc
      361 gtcctctacc atccgccgct ctccgcggcc gatctcggcg gcacggttcc cggcgcggcg
      421 ggcgcgctca cgttccgcgt gtcgcccagc ccgcgcgccg cgcaggtcct cctcgacacc
      481 ggctacgaaa acgtccgcct tttcccgttc acggaccagc tcgagcgcgg gcaggtgctg
      541 ggccctgcgg gcggctcggt gacgacggcg gacggcgtcg agctcgtcgt gcccgagagc
      601 gccctcgcgc agaagaccgt cgtctccgcg cggaagctca ccgcctccga gctggcgctc
      661 ctgccgcccg tcgcgggcta cacgaccgtc gcgggcgtgc gcgtcagcct ctcgggcgcg
      721 acgctcgctc gcgcggcgac gctgaagctg aacgcgcctt cgggcctccc ggccgacgcg
      781 gccgacgacc cgcgttacgt cctcgccgtc ttcgtcgaaa tgcccgacga cggccgcggc
      841 gcgttcgcga acgccgtctc gcgcgtcacg ctcctgcccg cagcgggcgc cgtgcccccg
      901 aagctcgtag ccgcgcccga ggcctcgggc tcgccgctgc ctttccccgg cgtcgcgggg
      961 gagggcgtct tcctctatct ccacgcgaac gcgcccgtcg gattcgtcac cggccgcgtg
     1021 acgtcgccga acggcgcggg aatcgccggg tcgcgcgtga cggccgcggg cctcggcacg
     1081 gcaaacctgt ccgtgcccgg cggcgtctac gccgtgcccg cgcccgcggg cgcgcccacg
     1141 ctgaacgcgc ttcaccccac gctcggcgtc gcgggctcgg ggcaggtgcc gtcgctcgcg
     1201 aagggccagg tcgcgacgct ggacctcgtc ctcacgccgg tgccgccgac gatccgcgcg
     1261 ctccagcccg ccagcggcgc ggtggaccag cccgtcggat cgacggtgtc cgtcgcgttc
     1321 agcgcggcgc tggatccttc cagcgtcacc gcctcgacgc tctccgtctc gctcgcggac
     1381 gcgacgacag gcgcgccgac cggtctcctg ttcaacgggg cgatctcgct ctcggccgac
     1441 aggacgaccg tcgtcttcac gcccgcgcgc ccgctcccgc ccggcaagcg catcgcggcg
     1501 cgcttcaccg gcggcgtccg cgacgcggcc ggcaccctct acggcgggac gctgcccgtc
     1561 gactggagct tcacgacctc cacgcaattc gtcacgggcg gcgcgatcca ccccgagaag
     1621 atcaggctcc tcgtgccggt gaacggcgtc gcccagatcg tcggcgccga cggcgcgctg
     1681 ccgctcgtca cgggcggcac gccgccgtgg tccgtctggg ccgacgtcga aggccccgtc
     1741 tgcccgtcga gggtcacgca gccggcgaac gcgaacggcg gtttcacgct gaacgccggc
     1801 tgcccgccca cggtcagcgt cacgattgcg agcaaggtct ggctgcacgt catcgacccg
     1861 acgggcgccg aagccgcgac gttccggctc ggcccgttcg ctacgcccga cggtaagggc
     1921 ttcgtggcgg cgccgggcga cgagaccgtg ttcacgaccg cggaaggcgt cgaggtcacc
     1981 gcgcccgcgg gcgcgttcga cgtgcccacg ctcgtcaagg tctcgaagca gccgctcgac
     2041 tcgctgaacg tcgcgctgaa tcccggcctc gagatgggcg ccgtcctcaa cgtcgacttc
     2101 gacggcaccg cgaaggagac gctgcgcctc cggatccccg tcacgacggc ctcgcccgtc
     2161 ggcggcctcg tcttcgcggg cacgcccatc gatcttccgt ggggaaggaa actcaagatc
     2221 ctcgacgtcg gccgcctcgt ggacgacggc aaaggcggca agctgatctc gacgctcgag
     2281 gcggaccagc cggacgaccc gtccctgacc gcgggcgcgg ccccgagctc cgccacgtcg
     2341 ggagggcgga cggcgctcgt ctcgcgcaag ctcctgcgca cgattgccgc cgagatgacg
     2401 gcccgtggtt ccgttgcgtt cgtcttcggg acgacgctgc ccctcatggg cgtcacggga
     2461 gcggtcgtct cggggactct cttcgttctc tacaacgcgc tcgccgacgc cttcgtcttc
     2521 caggctctcg cgaacgactg gagcggtcgg tacgtgctgc cggcctaccc gggccaggcg
     2581 ctctcggtga tcaagcgcga cacggcgacg ggctggatcc tcgcgcgtca ggactacgcg
     2641 aatcccgcgg gctcgagcgg cctcacgaac ctcggcacgc tcgacgcggg tccggacacg
     2701 ccgcccgtcc tcgtggatgc gagcccgatc cgcgtcctgc gattcaccgc gcctccggtc
     2761 gacgctgcac gtgggatcga cacgctgcgc ctcgcgctcg aaatggaagc gcgggtcggc
     2821 tccgaccgca cggcgtccct cgccagcgtg cctgggttcg cgttcgcgga cgggacgtcc
     2881 gtcgacttcc tcgacttcac gcaggcctcc gccaagacgc cgagcggcgc ggtctcggcg
     2941 cgcagcttcg tctcgggcgg cgccttcgcc accgggccgg tcgccgccca gcccgccgac
     3001 gacatgttcg tgtacgtctc gccgggcgag ctcgacccgg caggagtcag cgagctcacg
     3061 ctcacgttct cgcgccggat cgacaacccg accggcaacg ccgccgacct cgtcgcgctc
     3121 tgggactgcg gcgacgtggc gcgggttctc tccggctccg ccccgtcctg cccaggggcc
     3181 agcgtgaagg tcgcggccac cgccgatctc gcgctggccg gcgcgcagat cgtcgtccac
     3241 ctcccgggcc gcctcgccga gggccgccag taccggctgg atctcgtggg cgtgaagtcc
     3301 gccccgaagg gcatcgccta tccgcccggc gcaccgtcgt cgttcttctt cgcgacgcgc
     3361 cgcaccggcg atccgttcgg caagatcgtc acgtccgcgc tgggcgacac gaactcggct
     3421 cgcgacctcc tgaagttcgg ccacctgctg atggtcggct cggccaccgg tcgcctcgtc
     3481 gccgtggacg tgtccgaccc gaagaacccg acgacgtggg cttccctcgt gaacgtggac
     3541 gcgaccggga ccgcgacgga cgcgaaggcc gacatgctga gggcgttcgc gacggacggg
     3601 cacggccgcc tcttctacaa cgtccagttc ggctcgagct gggcggtccg gacggtgagg
     3661 atcgaggaca tccgcgagca tgccgctccg tgcccggcga cgccgttctt cccgggtgtc
     3721 ccctgcttca cgagcatcga gggcggcgcc cgcacggcct tcgccccagg ggcgatcggc
     3781 acgctcctcg gaccggatta cctcgccctc gtcgggagcc tgccgacggg aacgccgtcg
     3841 tcgatggaag tcgtcgtacg ggacgagtcc gagccgccgg cgaacggttc ctacgagctc
     3901 aaggagctgt acgagcgtcc ggaacagcac ggcgccgatt ccttcaagag tctggcggcg
     3961 gtgaacgggt tctacgaatt cgacgtcaag gtcttcacgg agactcacct cgccaatcgg
     4021 ccgtcctcgt gcggcgagac gccgtgggac cgctaccagc gcgtctccgt cgacaatctt
     4081 tcgacgggcc agacctggtc gaaggacgtc gagaacctcg gcccgcgggg ctccgtcaac
     4141 gagaaaatcc gcatccgcgg gcggctcggc gaccggctgc gcatccggta caacgtcctc
     4201 gcgctcggct acctctcgat cgtcggcagc ggcgtctcgg tgctcgacct gaaccggatg
     4261 taccgcaacc ccgggcccgt cgcggcgcag gcgaacaaga gccagtgcga ccggcgcatc
     4321 ggaaagtacg aaggcgccga cgtcgccttg tacagtgtct gcccggcgcc gaatagcgcg
     4381 ggcgcggctc aggcggcgcc ggcgctgaac ggcctctcga acacgcccgc tctcgtcgcc
     4441 gtgcccgggc ccgccaacgt cgacgtccac tcggtcctca cgcacttcgg cgtcgtccac
     4501 gagagcgcaa aactcaccac tccaggtgac ctctccatac cctcgaaagc acccctgcaa
     4561 tcctggcaga cgcccgacgg cgcgacgtgc ctcggcgacc gtgcgccggc cggcctccgc
     4621 ccgtcgtacc gcgcgatcgc cttcgcacgc gacgccgcgt ggtcagacct cggcatccgc
     4681 gccggatcgg gtgcagcgtt catcgggccg gcgctcaagc ccatcaggac gacgggcgac
     4741 ctgctcctcg tgtcgatggg cagcgcgggc atcctctcga tctcgctcgg accgctggct
     4801 aacggcgatc cgatcggccg ctactgggtc gaggggcact ccgtctaccg gctccaggcg
     4861 gacgtgtcgc gcggccttct cttcgcgggc ggccaggaca ggaacggcaa ctcgatcgtg
     4921 gacgtttggg atctcgcgcg cgcgaacggc ggccccactc ccgacggcgt tttcccgccg
     4981 atctcggggc aggccgtcga cccgcgcctg ctcgcgacgc tctacgtgcc gtgggacacg
     5041 aaccacatcg gcctcgacga gaccggcgcg ggcctcgtct acacgtgggg ttaccgcagg
     5101 gcgccggacg gcagcggcgg cacggtgacg gaggaaggcg cgttcgtgat gcccttcgac
     5161 gcgccgtcga tcacgttcgc gggcgtgttc cgcaaggaca ccgcggcgcc ggcggcggga
     5221 acgctcgctc cgagcgttcg ccagctcgtc acgaagctcg acccgctggg cgttccgctg
     5281 cgcctctccg cgttcgacga gaggaacacg ccggcggacg agacctgctc cgacctcgac
     5341 cgcgcgctcg gcgcgaactg ccgtacggcg gcgttcaagg tgcgcgtcgc actgccgggc
     5401 tcggcggcgg gccgcgcgtt gggcgcgacg ctcaacgcga aggtccagag ccttcggggg
     5461 ctgcccgaaa agcgcctcct cgcgcaggaa gacctcggcg ccctcgtccc gccgtgcggc
     5521 ggcaccgagc cgtgcgacgg cggcgcgcat gcggcgcagt ccggctggcc ggaccgcgac
     5581 gtgtgggtga cgctgcggcg gatcgggaag gggtactcgg gcgcggacat cacgagcccc
     5641 gacggcccga cctcgaacgc gtacgacctc tacgaatcgg acgagacggt cgtcctcgtc
     5701 gcggaccctc gcgcgcggtt cgcttactgg aagccttatc aggccctcga ggtgaagtcc
     5761 ggcaatccgc cggacgaacg tcaccagtgc cggcgctgcg accggccgac cacgctcccg
     5821 caggacgcga gcgacacgag cgtcgtcgag cttctcgccg gcggcccgta cgtccgggcg
     5881 ttcctctcgc cgaagccggg ctcgcccgac gagctcgcga cgcgcgccgc catcctgtac
     5941 ttcaacggca cgaactaccc gccgccctcg ggttacgtcc gcgtgagcgg cttcgccgac
     6001 gaggtcccgt cggtcacgca gatcgcgctc gccgagccgg cgcagaaccc cgccgtctgg
     6061 acggcggaag cgggggccag cgtccgtctc acgtcgggag agggcgtcgt cgagacgcgc
     6121 gacttctcgg cagcggcccg gtcgctcggg ttcgcgctgg accgcacgta ccggtccggc
     6181 gtcgccggct acggcccgct cggcgccgcc ggctggaacg cttccctctt cgcgcacctc
     6241 agggaaaccg ctctcatccc gcccgtcccc ggcggcagct cgtcgtacgt cgactaccac
     6301 gacggcgccg ggcaggtctg gcgcttcgtg ggacttcctg ccgatgtttc gtgcccggcc
     6361 ggcaccgcgc gggacggtgg cgagacgtac tgcacgccga aaggcctcta catgcggctc
     6421 gacaagctga cggacgggac cttccgcctc gtcggccgca acaacgacac gctgcgcttc
     6481 gacccggccg gccgcctcac gatgatctcg gaccggcacg cgcaggggga tcccgacaag
     6541 aaggggagca gcgtccgcct ctactacacg cccgcgggcg acctcgtgcg cgtgcaggac
     6601 gagatggggc gcgtctaccg gctgacgtac ttcgactcca ccgatcccgc gaacgctggg
     6661 ctcctcaaga agatcgagga cttttcgtcg cccgcgcgaa cggtggagtt cgagtacgac
     6721 cggcggcggc tctcgaaggt cacgctgccg aaggtcaccg tcgtcccgtc gaaactcgga
     6781 atcgtgcccg gtattccggt catcagttac gggtacgggg cctcgacgaa cgcgaacacc
     6841 gcgccgccgc tccacggacc gctctggagc ggaaagctcg agacgttcac gctccccggg
     6901 gagacgaccg agcgaatccg gttcacgtac gaccctacgt cggggcgggc gagcccggtc
     6961 ctcgcgggca aggcgacgac cgtgacgtgg aagctcacgg cgacgcccgc gaccgcggag
     7021 cccgcgaagt cggtcgaggt gctcgacccg agcggcctcc ggacgacgta cacgctcgac
     7081 gacaagggcc acgtcgccaa cgtcctccgc aagggcgtcg aggtgagcaa gggcggcgcg
     7141 cccgcgctca ccgatctcac ggagtcgtac ctctatcgcg acgatggccg catctctttc
     7201 gcgacgcgcg cggacgggac gaagatggat tacgcgcctc cgccggccgg gcctcagcgc
     7261 ctcgcctacc ggatcccgtc cgtcaccgag atcgggaaag acccggacgg ctcgatcaag
     7321 cgcgggttcg agtaccaggg cgacgggctc gtcgagaaga tcaccgaccc cgagggccgc
     7381 acgatcacgc cgggcgtgcc ggcggcgggg ctcaaggtcg actcgaagac gagcgatggg
     7441 ctgagcagct cgacgacgtt cgacgacttc ggccggccga agacctacgg gggcggcgcg
     7501 ggcacgaagg gggaaacgac ctaccgcgac gacgcgctcg gcgtcaaggg cggaggtttc
     7561 ccgctcgagg tcgtcaaggg agacaagccc tccggcaagt acgtcggcgc cgagttcgag
     7621 agggacgagc gcggcaaccc gaccgagatt cgcgagtcgt cgggcgccgt cacgaagatc
     7681 gactacgacg agtgggaccg gcccgtgcac gaaacgttcg gcaggggcgg cgagcaggaa
     7741 gcgccgggcg aggtcttccg cgggttcgac acggccggcc gcctcaagaa ggagatccgc
     7801 acccagctca acgccggaga cgtgacgacg acgtgggagt acaacgaccg cgatcaggtc
     7861 acggagataa agcgcgacca cgttgccagc gccacgccgt tcgcggcgcc ggacgccacg
     7921 tcgacgacga agttcacgta cgacgtgacg agcgggttcc tcaagtccgt ctcgaccgac
     7981 caggggacga cgacgaccta ccggttcgag agggcggggc ggctcgcggc ggtgacgccg
     8041 gacggctcgg gcacgcgcca gttcggatac gacgaactgg gccggccgac gtacgtgttc
     8101 gacggggaca agggctcgtg gacaggcgcg tacgactcgt tcggccggct gggcacggaa
     8161 aacctgcccg ggggcggcat cgtcacgcga acgttcgaca aggcgggcgg cctcaagacg
     8221 gcgaagctcg cggacggcct cggagccgtc ctctcggaga ttcccttcgc caaggtgacg
     8281 agctggggtg cgctgacgaa cctcgagcgc cgggtgacgg ctggcacctc gctcctcatg
     8341 acgcgcgaac tcgacgccga ggggcggctc acgctgcaga tgtccgacgc tcacaaggac
     8401 ctcgagatga ggttcgaggc gggcacgggc cggcccgcct tcgtcggcga cgccgtgaac
     8461 gagctcacgc tcggctacgg gagcgccgcg accgcctggc cgaagtccct gacgtacggc
     8521 gtcgccgtgg acccttcgaa ggccgcggtg ccgacggtga tcagcacgct gaagcacgac
     8581 acgctggggc gcgtcgtcga ggacttacgc gacgacggca cactccggac cgcgcgcttc
     8641 gacgaagagg gccgccccgt cgaaaccacc gacggcgcct cggcgacgtc gcttcgctgg
     8701 gactcgcgcg gagcgctcgt ccagctgatc cgtcccgccg gccagggcgc gaccggcctc
     8761 gggtacgacc acgacgggcg attgacggcg aagaaaacgg atgcgggcgg cacgtcgtat
     8821 gtgtcgatgt acgactatta cgcgcccggc gagagccatg cgggcctgct caggaccttc
     8881 gcccggacgg acggcccgac gctcacggtc aaggagtact acccggacga catggccaag
     8941 accgtgaagc tgagcgacgg cgcgaccgtg ggcctcgagt acgacgacgc gaaccgcatg
     9001 acgaagacga cggtgacggg cgggtcctcc gtgccgggcg gcgtcctcta cgcgtgggac
     9061 gccgcctcgc ggatgcgggg cgcgtcccgc ctcatgccga acggcatcga cgtcgacgcc
     9121 gcggccgccg tcgtcccggg cgcgtacgac ttcgcggggc ggccgcacac cgagaccgtc
     9181 ggctcgcgcc ccgcgctcac gcgcgactac gacctcctcg gcggcgtcac gtcgctgggg
     9241 ctgccgctcg gggtcggcgc cgctcgcccg ttctcgtaca cgcgcgtgtt cgagcccctc
     9301 acgcaccgcc tcgcgtcggt caccggcgcc ggcgacgcgc cgccgcccgg cggtgtcgcg
     9361 ccggcccttc tcggcgcttc gtgggcatgg gtcggcgacg accgagtcct cggagtcacg
     9421 tccaacggcc cgctccacgc cgcacaccgc ttcgggtacg tcggcggccc gggcggaccg
     9481 gacggctccg tgcccggcgc cgcgaagtgg aagctcggaa cgctcacggt cggcacggac
     9541 ccgggcgcta agaacctcct tccgttcggc gatcccctcg cgccgggcgc ggccccgtgg
     9601 ggccagttcg cgtatggcta caattcctcc atcacgggag acggcacgaa gctcggccgg
     9661 agggtgacgg gcgtggggct tgcgggcctc cttgccgggt ctctgctgtc caacctcggc
     9721 tacgcctacg ggctcgacgc gtccaagcgc ctcacgacgg cgtacgccgg gccgggcagc
     9781 gtcacgggcg ccgacgcggc ggccgcgatc gccgccttcg agcgcttcaa gtacgagtac
     9841 ggctccgccg accagctcca gaaggagacc cgcgaaacct ccggccgcag cgtcggctac
     9901 gaaaccggcg gcgacggccg gcccgactcg cgccagctcg acgcgggacc caagtccctc
     9961 ttcctctacg acgcgctcaa gcgccgcacg ggcgacgacc gcttcacctt cacctggcac
    10021 tacgacggca agatcgccac ggccgtcgtc aaggactgct ggccgatcga aaacggcgaa
    10081 tcgcaggctc ccggctgcga ctcgggctac acccggcctg cccaggccgg ccacaagctc
    10141 gtcttcgcgt acgacgcact ctcgcgcctc ctcacgcgca cgcacctcgg cgagatcccg
    10201 tccgggggcg gcgagggcga tcgccccttc atcgaaaccc gcgagtacct cttcgacggc
    10261 aacaccctgc tcgccgaggt tgcgaaggcg aaggacggtg ccatccgctg gaggaagacc
    10321 tacgtccccg gcgccgacct ccacgaccac gtccaggtca gggtcgaaac ctacgacgcc
    10381 gcccagaacc tcgtctccga ccgcctctac gcctacatca gggacgagca gggcacggtc
    10441 ctcgcgctgg ccgacgaagc cagcgacccg acgcacccgt ccatccccat ccgctacctc
    10501 tacaccccgt acggcaacgc ccacgccgaa accgggccgg agcttcggaa ggggagctac
    10561 ggcttcggga tcacgagcgt caaggacgcg agtggagcga cgaagacgca gacggcgacg
    10621 gggacgctgc ccggcggaat ccgcctgagc ttcgggatcg acgtcgacct ctcgacgctg
    10681 gcgaccggag tcgccttcga aaagcgggcg acggacggga cgtggggagc gctgacggag
    10741 atggcgattg gcagggcgcc ggatgccgca aacagcgacg tcgacatcct cccgctcggc
    10801 gggtttgcgt tcgcgacgag ctatcgcgtc cgtacgacgg cagctttgaa ggacgttctc
    10861 ggcaggacac tggccgaccc gaaggtgctc gagctgccgc aaacgccgcc ggtgattgcg
    10921 ccgggcgaca tcggcgcgag cagctacgag tacgtgagga cgttcccggt cgagtacgac
    10981 agcctcgaag cggctgccaa cgacgcgaat ggcctcttcc ccggcggcca gccgatgggc
    11041 ttccaggggc agtacacgga cccgctcctc ggcttcctgc tgacaccgaa tcgtgtctac
    11101 gacgcgagga atggggtttg gctctcccag gatccattgg gagataagga ctccccgaac
    11161 ctctacggct tcg
//
LOCUS       DAOMFY010000114        11163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1805_length_11163_cov_6.357130, whole genome shotgun sequence.
ACCESSION   DAOMFY010000114 DAOMFY010000000
VERSION     DAOMFY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11163
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1805_length_11163_cov_6.357130"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLB01_10575"
     CDS             <1..352
                     /locus_tag="PLB01_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77790.1"
                     /translation="AVLGRAEGRGFVPARPGKAAPEKALATVDLAGEAEGTARTATFF
                     AAPAGSAAGLVAVQVTGRPERLLVEKTVLDEIRREAAALRDEAEGKSKEPKKAKAPEP
                     TPVPGGLVKGPVVR"
     gene            complement(356..1879)
                     /locus_tag="PLB01_10580"
     CDS             complement(356..1879)
                     /locus_tag="PLB01_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQQ77791.1"
                     /translation="MNPEHLELLQTPEWNEWRAARPAFTPDFTDMRLLNADLSGKNLS
                     GADLSRSKLEGCNLEGANLSRVHVTGASFNGSKLDRADLSDTDLDYLLLDRVSFVGAI
                     FRNTTLRSATVREAVMKAADFTGAKLDGTDFTATDFSYGRLLYANFRGAGIDRCVFEG
                     CDLTQAIFDGVTYKKGNFDKANLTLAFFRDAKLTGTSFKGAKLQRTVLRGSDLSGTDF
                     TGADVSEADFERSILQASNFTDAVLRKTSAHRADMRNALFANAQMEETDLTGAFLDKM
                     DLRTPRMKLVNFTGARLIEAVGARANLRGSNFTEADLSGADLQEANLEGCILPKAKVA
                     RANLRKANLKRGRLSEANAVGANLSGANLFGIEAENADFSASDLRFAQLVGAKLDGAQ
                     FKQANIGGADFTEARLGGSSFVGAFGENAKFTGQFHLKLVLAASSLLGSAKKKTEQME
                     KSKEVERLQRLAALEKAADERARAMGYRKEDGGSASKSSASLTMTGTRPSPGTPKKG"
     gene            1916..3406
                     /locus_tag="PLB01_10585"
     CDS             1916..3406
                     /locus_tag="PLB01_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQQ77792.1"
                     /translation="MAETPVRGPERLRRALLGGYPLLYVVSWEEGRVERAVQALAQKF
                     YEKPVAYSVWTCVDGLVGGGGDRKPDTTDPIKALDAILADPAPGFVLMKDLTAHLPRP
                     EVTRRLRDAYRALKGRGRFLILVSPRLVVPDDLRKEIYVLDFDLPDETEILVLLSHLG
                     KRYFGEKGLAETDAKKLAMALKGLTSDETEHVVSKVFSRRPVFDEAGYFEVLAEKEQA
                     TRKDGVLEFVPPRFSLADIGGLENLKEWLVKRKNLFTKEALDAGMPVPKGILMMGMSG
                     CGKSLSVKAISALWNLPLFRLDMNLVFGTDNPEQTFERALRTVESLSPALLWIDEIEM
                     AITGGREAGQGDASLGRIFSTFLTWMQEKQGLVFVAATANRIHLLPAEVIRKGRFDQV
                     FFIDLPNENERKQIFNVHVKKVGGDPSLFDVVFLAKATKGFVGSEIEALVQAAAIEAY
                     NQGRKLSEDDLSKMITDTVPLSRTMDEQIKAIKSWAHDRAMPASKA"
     gene            complement(3422..3781)
                     /locus_tag="PLB01_10590"
     CDS             complement(3422..3781)
                     /locus_tag="PLB01_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HQQ77793.1"
                     /translation="MTHPMTTTTFTLDGYRIARTLGVVRGITVRSRSIFGTIGGSLQT
                     LVGGNISLFTELCEKTRNEAFDMMLAHARDVGANAVVGVRYDATELMQGVTEVLCYGT
                     AVVVAPEAGSPPPPPLP"
     gene            complement(3778..4728)
                     /locus_tag="PLB01_10595"
     CDS             complement(3778..4728)
                     /locus_tag="PLB01_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQQ77794.1"
                     /translation="MSFRDVRVEDVLAGTFLALLASVIGLASLRASFDTVEIFWWDLC
                     FIVAPAAILAFLAAVRYGFRLRGAAGFAPVLGVLRDWFPFAFFSFTYELFRLSSWTTV
                     LSRDRDVELLRIDRWLFGETPAVSLQQLAGPLMTDVMTGAYSLHLVLPPVLGMLLYAR
                     QKRLFREFLLAVLLSGILGQTGYLLVPAVGPKRAFPELFSRTLEGRVYEPVTSALEAV
                     RAPRDVFPSLHVGISAIVLAFAWRRSRRLAGALAPLVLLNWVSTLYLRYHYFIDVVAG
                     WATAWASIALAGRLLALEDRIRARWAPAAAAPPADTGGPP"
     gene            complement(4715..5518)
                     /locus_tag="PLB01_10600"
     CDS             complement(4715..5518)
                     /locus_tag="PLB01_10600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77795.1"
                     /translation="MSPFLVSLAIGRLVPILLATTAVLVLARICADRFASSARMNRVL
                     FAAILSAGLAVQAPYGLRSVTLWRAEHAISRGQTERAARSIEAFRNLGGTPGAWRGAR
                     WMGFLVRERRWGDAHDLAVALLAEPRPAGGTARADLSLTLGISQYYLGDVPGAAASLR
                     AAAGADPRYAYLARYFEGRVAERGGDVRAAVDSYASALSASPRFGPALYQIVRLLLAA
                     GRKDDAAGVVGQVLAASPAAAGDALLQAARGHIAAGTIPPEKEFEIVLP"
     gene            complement(5523..6191)
                     /locus_tag="PLB01_10605"
     CDS             complement(5523..6191)
                     /locus_tag="PLB01_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQQ77796.1"
                     /translation="MSGRRLVRLFPLPNVVLLPGTTIPLHVFEPRYRRMLADALDSDR
                     LIGMQLLKPGDSADGARPAVYSIGCAGEVVHHEPLEDGSSNLLLRGAFRYRIESEPHT
                     DAPYRVAEVTPIGIAPLPAADRAGRTPRDLRRALADGVRRLADGVGRPAARELPVRLS
                     DEDLVNEAIGRLGLEPEETYQLLAMNRLEERYAWTLAHIEGVQRRLDFLSPFRRPGGD
                     SRWN"
     gene            6252..6485
                     /locus_tag="PLB01_10610"
     CDS             6252..6485
                     /locus_tag="PLB01_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HQQ77797.1"
                     /translation="MANKDDRYAENAPGKFYVDTQCIDCDVCRVTAPNNFTRQEEKGY
                     SYVSKQASTPEEEAQCQEAMDSCPVEAIGNDGE"
     gene            complement(6528..7418)
                     /gene="lipA"
                     /locus_tag="PLB01_10615"
     CDS             complement(6528..7418)
                     /gene="lipA"
                     /locus_tag="PLB01_10615"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQQ77798.1"
                     /translation="MSADSRPRYGVTPRPEWLKVRMPASGFESEITRLVEDKSLNTVC
                     QEARCPNLAECWGVERTATFMLMGDVCTRRCGFCAVTQGIGRPLDPEEPRHVAEAVRT
                     LGVAHAVVTSVNRDELPDGGAAHFAATIRAIRALAPGVTVEVLIPDFLGSAAALGTVL
                     DEAPEVLNHNTETVERLYRRVRPDAIYARSLELLRRAAEKRDREKISLKVKSGVMVGL
                     GETREELSATLRDLRASGVDVVTVGQYLQPHARRLPIERYYTPQEFEEIRKEGEEMGF
                     GVVESGPLVRSSYHARRALD"
     gene            7484..8041
                     /locus_tag="PLB01_10620"
     CDS             7484..8041
                     /locus_tag="PLB01_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77799.1"
                     /translation="MVSIASLWLPILLSAAAVFVVSSIIHMFLGYHSADYRKLPAEDD
                     FMAAVRKLDIPPGDYMVPCPASHAAFKDPAFQEKFKAGPRAMMTVFGPYEGMGAQLAQ
                     WFLYAILISVTAAYISGRALGPGAHYMKVFRFAGATAFYGYALAHLQASIWYKKSWGT
                     TFRNVFDGLVYGLVTAGFFGWLWPR"
     gene            complement(8038..8718)
                     /gene="lipB"
                     /locus_tag="PLB01_10625"
     CDS             complement(8038..8718)
                     /gene="lipB"
                     /locus_tag="PLB01_10625"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQQ77800.1"
                     /translation="MRRTDVVSLLPEGRVSWDAAFALQKQAAERVKEGGPETLFLLEH
                     ADVVTVGRNAGLGELLVSEDFLRQKGIALRVADRGGKLTFHGPGQLVAYPVLDLSPDR
                     QDVRRYVRDLEEVLIRTARDFGVEAARSPAPARWASVWVGNDKLAAIGVHLSRWITTH
                     GVALNVSTDLARFSLFVPCGIADGGVTSLAKTSGAPPPPLGAVGGRFVSHFAEVFERE
                     MVERGTRP"
     gene            8834..9328
                     /locus_tag="PLB01_10630"
     CDS             8834..9328
                     /locus_tag="PLB01_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77801.1"
                     /translation="MKRRLCLTILAALTLASAAPRLGAQEQAREPEPSARTAPLAGRS
                     PLIFASPIHGGCYIAAPGQCKIHVEPFTINISSGTKLALFKLVSIQLPGNVQTTIYDW
                     RPDQSNPAPASGTTYTPSLVTQDFAATCGNSYQISLQGRDTGDVNTFNLGLTGTFTCP
                     TTVP"
     gene            9334..10143
                     /locus_tag="PLB01_10635"
     CDS             9334..10143
                     /locus_tag="PLB01_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77802.1"
                     /translation="MRPVRSAVVLAAAIAAGAARGEEREAAVRSPLIFASPIHAGCKT
                     YNARTCQIVVEPFTINLAAGKKMVSFQVSVDGSVVYDWRPDQSNPAPALGSTYTPSRV
                     ALGFGVTCGKSHVVSLQGRDTGDPNSFNLGQTSPITCPPPVLQLTFYALPPCRLIDTR
                     KSSGPDAGAPVLSGGELRVVGTAQKCGVPPTAAALSMNVTVTAPTTGGFFTLYAADGT
                     FPVASQLSFGAGQTRAGATIVPLALDASGFRILNGAAGTAHLIVDVNGYFE"
     gene            complement(10148..>11163)
                     /locus_tag="PLB01_10640"
     CDS             complement(10148..>11163)
                     /locus_tag="PLB01_10640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQQ77803.1"
                     /translation="SSPVVRAIAEDKGVDISQIPGTGISGRVTKQDIEAFLAGAGTGR
                     EDSLKVERAAGPAAPPAPVAAPSPAPSQKSESPAPLAARPVEAPLAARPASTIAFPGG
                     NVTIEPMSAMRRKIAQRMVESKQTSAHVTTVFEVDYSGIAKLRNRVKARFEEQYGTKL
                     TYLPFLLRAAIAALKARPLVNASIVGDDIVFHKEVNLGIAVALDWGLIVPVIRRADDL
                     SLVGLARAANDLAARARAKKLLPDEVAGGTFTVTNHGSFGSLFATPVINQPQVAIMGV
                     GTIEKRAKVVEMPDGSDSLAIKTMGYVALTFDHRLIDGSEGDAFVNVVKSTLEEGTWP
                     ELE"
BASE COUNT         1727 a         3844 c         3946 g         1646 t
ORIGIN      
        1 ggccgtgctc ggccgggccg aggggcgcgg cttcgtgccg gccaggccgg ggaaggccgc
       61 tcccgagaag gcgctcgcga cggtcgacct cgccggagag gccgagggca cggcgcggac
      121 cgcgacgttc ttcgcggcac ccgccgggtc ggccgccggc ctcgtggccg tccaggtgac
      181 cggccggccg gagcgcctcc tcgtcgagaa gaccgtgctc gacgagatcc gccgcgaggc
      241 cgccgcgctc cgggacgagg ccgaaggcaa gtcgaaggag ccgaagaagg cgaaggcgcc
      301 cgagccgacg ccggtccccg gcgggctcgt caagggtccg gtcgtccgtt agcggtcagc
      361 ccttcttcgg tgtgccgggc gacggccgcg tgcccgtcat cgtcaggctc gccgacgact
      421 tcgaagcgct gccgccgtct tccttgcggt agcccatcgc cctcgcccgc tcgtccgccg
      481 ccttctcgag agccgcgagg cgctggagcc gctcgacctc cttggatttt tccatctgct
      541 cggtcttctt cttcgccgag ccgagcagcg agctcgcggc gaggacgagc ttgaggtgga
      601 actggcccgt gaacttcgcg ttttcgccga atgccccgac gaacgacgag ccgccgaggc
      661 gcgcctcggt gaaatccgcg cctccgatgt tggcctgctt gaactgggcg ccgtcgagct
      721 tggcgcccac gagctgggcg aagcggaggt cgctcgcgga gaagtcggcg ttctcggcct
      781 cgatgccgaa caggtttgcg cccgagagat tggcgccgac ggcgttcgcc tccgagaggc
      841 ggccccgctt caggttcgcc ttccggaggt tcgcgcgcgc gaccttcgcc ttcggcagga
      901 tgcagccctc gaggttcgcc tcctggagat ccgcgccgga gaggtccgcc tcggtgaagt
      961 tcgagccccg gaggttggcc cgggccccga ccgcctcgat gagccgcgcg cccgtgaagt
     1021 tcacgagctt catccgcggc gtgcggaggt ccatcttgtc gaggaacgcg cccgtcaggt
     1081 cggtctcctc catctgcgcg ttcgcgaaca gcgcgttcct catgtccgcg cgatgggcgc
     1141 tcgtcttgcg gagaacggcg tccgtgaagt tcgacgcctg gaggatcgac cgctcgaagt
     1201 cggcttcgga gacgtccgcc cccgtgaagt ccgttcccga gaggtcggag ccgcgaagga
     1261 ccgtccgctg gagcttcgcg cccttgaagg acgtgcccgt gagcttcgcg tcgcggaaga
     1321 acgcgagcgt gaggtttgcc ttgtcgaagt tgcccttctt gtacgtgacg ccgtcgaaga
     1381 tcgcctgcgt gaggtcgcag ccttcgaaga cgcaccggtc gattccggcg ccccggaagt
     1441 tcgcgtagag gaggcggccg tacgagaagt ccgtggccgt gaaatccgtg ccgtcgagct
     1501 tggcgccggt gaagtccgcc gccttcatca cggcctctcg gaccgtggcg ctccggagcg
     1561 tcgtgttccg gaagatggcc ccgacgaagg agacccggtc gagaagaagg tagtcgaggt
     1621 cggtgtcgga gaggtccgcg cggtccagct tcgagccgtt gaacgaggcg ccggtcacgt
     1681 ggacgcgcga gaggttcgcg ccctcgaggt tgcagccttc cagcttcgat cgcgacaggt
     1741 cggcgcccga gaggttcttc cccgagaggt cggcgttcag gagccgcatg tcggtgaagt
     1801 cgggggtgaa ggcgggccgc gcggctcgcc actcgttcca ttcgggggtc tggagcagtt
     1861 caagatgttc gggattcacg caccgtagtg tatcgaagcc tgctagattt gttccatggc
     1921 cgaaactccg gtccggggcc cggagcgcct gcggcgggcg ctcctgggcg ggtatccgct
     1981 cctgtacgtc gtttcgtggg aagaggggcg ggtcgagcgc gccgtccagg cgctcgcgca
     2041 gaagttctac gagaagccgg tggcctactc ggtgtggacc tgcgtggacg ggctcgtcgg
     2101 cggcggcggc gaccgcaagc cggacaccac cgaccccatc aaggccctcg acgcgatcct
     2161 cgcggacccc gctcccgggt tcgttctcat gaaggacctg acggcccatc tcccgcgtcc
     2221 cgaggtcacc cgccggctcc gggacgccta ccgggccctc aagggccggg gccggttcct
     2281 gatcctcgtc tcgccccgtc tcgtcgtccc cgacgacctc cggaaggaga tctacgtcct
     2341 cgacttcgac ctgccggacg agaccgagat cctcgtcctt ctcagccacc tcggcaaacg
     2401 ctacttcggg gagaaggggc tcgccgagac cgacgcgaag aagctcgcga tggccctcaa
     2461 ggggctgacc tcggacgaga cggagcacgt cgtctccaag gtgttctcgc ggcgtccggt
     2521 gttcgacgaa gcggggtact tcgaggtcct cgcggagaag gagcaggcga cgcggaagga
     2581 cggcgtcctg gagttcgtcc cgccgcgctt ctccctcgcc gacatcgggg ggctcgagaa
     2641 cctcaaggag tggctcgtca agcgcaagaa cctcttcacg aaggaagcgc tcgacgcggg
     2701 gatgccggtc ccgaagggga tcctcatgat ggggatgtcg ggctgcggca agtctctctc
     2761 cgtcaaggcg atctccgcgc tctggaacct tccgctcttc cgcctcgaca tgaacctcgt
     2821 cttcgggacc gacaacccgg agcagacgtt cgagcgggcg ctgcgcacgg tggagtccct
     2881 ttcgccggcg ctcctgtgga tcgacgagat cgagatggcg atcacgggcg ggcgcgaggc
     2941 ggggcagggc gacgcctcgc tcgggcgcat cttctcgacg ttcctcacgt ggatgcagga
     3001 gaagcagggc ctcgtcttcg tcgcggcgac cgcgaaccgc atccacctcc tgccggccga
     3061 ggtcatccgc aagggccggt tcgaccaggt cttcttcatc gacctcccga acgagaacga
     3121 gcgcaagcag atcttcaacg tccacgtgaa gaaggtcggc ggcgacccga gcctcttcga
     3181 cgtcgtgttc ctcgcgaaag cgacgaaggg gttcgtcgga tcggagatcg aggccctcgt
     3241 ccaggccgcc gcgatcgagg cctacaacca gggccgcaag ctctccgagg acgacctctc
     3301 aaagatgatc acggacacgg tccccctctc gcggaccatg gacgagcaga tcaaggcgat
     3361 caagagctgg gcgcacgacc gcgcgatgcc ggcgtcgaag gcctgaggca gcgacctccc
     3421 gtcaggggag gggcgggggc gggggagagc ccgcttccgg cgccacgacg acggccgtcc
     3481 cgtagcagag cacctcggtc acgccctgca tgagctccgt ggcgtcgtaa cgcacgccga
     3541 cgaccgcgtt cgcgcccacg tcccgcgcgt gcgcgagcat catgtcgaag gcctcgttgc
     3601 gggtcttctc gcagagctcc gtgaagagcg agatgttccc gccgacgagc gtctggagcg
     3661 agccgccgat cgtcccgaag atcgagcgcg agcgcaccgt gatcccgcgc acgacaccga
     3721 gcgttcgcgc gatgcggtag ccgtccagcg tgaaggtggt cgtcgtcatc ggatgcgtca
     3781 tgggggtcct cccgtgtcgg cgggtggcgc cgccgctgcc ggcgcccacc gcgcccgtat
     3841 tctgtcctcg agggcgagga gccggccggc cagcgcgatc gaggcccacg cggtcgccca
     3901 gcccgcgacg acgtcgatga agtagtggta gcgcaggtag agcgtggaga cccagttcag
     3961 gagcacgagc ggcgcgagcg cgccggcgag ccggcggctg cgccgccacg cgaatgcgag
     4021 cacgatcgcc gagatcccga cgtgcaggct cgggaacacg tcgcggggag cgcgcaccgc
     4081 ctcgagggcg ctcgtcacgg gctcgtagac gcgcccctcc agggtccggg agaagagctc
     4141 ggggaaagcg cgcttcggcc cgaccgccgg gacgaggagg tagccggtct ggccgaggat
     4201 tcccgagagg agcacggcga gcaggaactc gcggaagagg cgcttctggc gggcgtagag
     4261 cagcatgccc aggaccggcg gcaggacgag gtgcagcgag tacgccccgg tcatcacgtc
     4321 cgtcatcagg ggaccggcaa gctgctggag tgagacggcc ggagtctcgc cgaagagcca
     4381 ccggtcgatc cggaggagct cgacgtcgcg gtcgcgcgag aggaccgtcg tccaggacga
     4441 aagccggaac agttcgtagg tgaacgaaaa gaacgcgaac gggaaccagt cgcgcaggac
     4501 gcccaggacg ggcgcgaagc ccgccgcccc gcgcagccgg aacccgtacc ggacggccgc
     4561 gaggaacgcg aggatcgccg ccggcgcgac gatgaagcag aggtcccacc agaagatctc
     4621 gacggtgtcg aaggacgcgc gcagcgatgc caggccgatc acggaggcca gcagggcgag
     4681 gaaggtcccc gcgagcacgt cctcgacgcg cacgtcacgg aaggacaatc tcgaactcct
     4741 tttccggcgg aatcgttccc gccgcgatgt gaccccgtgc cgcctggagg agcgcgtcgc
     4801 ccgccgcggc gggcgacgcc gcgagcacct ggccgacgac gccggccgcg tcgtccttcc
     4861 gcccggcggc caggagcagc cgcacgatct ggtacagcgc ggggccgaag cgcggcgagg
     4921 ccgagagggc cgacgcgtag gagtccacgg cggcgcggac gtcgccgccc cgctccgcga
     4981 cacgcccctc gaagtagcgg gcgaggtacg cgtaacgggg atccgcgccc gcagcggcgc
     5041 ggagcgatgc cgcggcgccg ggcacgtcgc cgaggtagta ctgcgagatc ccgagcgtga
     5101 gggagaggtc ggccctggcc gtcccgccgg cgggccgcgg ctcggcgagc agcgcgacgg
     5161 cgaggtcgtg cgcgtccccc cagcgccgct cccggacgag gaagcccatc cagcgcgccc
     5221 cgcgccatgc cccgggcgtt ccgccgagat tccggaaggc ctcgatcgac cgggccgcgc
     5281 gctcggtctg cccgcggctg atcgcgtgct ccgcgcgcca gagggtcacg gatcgcaggc
     5341 cgtacggggc ctggacggcg agcccggcgg agaggatcgc ggcgaagagc acgcggttca
     5401 tccgcgcgct cgaagcgaac cgatccgcgc agattcgcgc caggacgagc acggccgtcg
     5461 tcgcgaggag aatcgggacg agccggccga tcgcgagcga aacgaggaac gggctcatgc
     5521 cgtcagttcc accgcgagtc cccgccgggg cgccggaacg gcgacaggaa atccagccgc
     5581 cgctggacgc cttcgatgtg cgcgagcgtc caggcgtagc gctcctcgag gcggttcatc
     5641 gcgaggagct ggtacgtctc ctccggctcg agcccgaggc gcccgatcgc ctcgttgacg
     5701 agatcctcgt cggacaggcg gacgggcagc tcgcgtgccg ccggccggcc gactccgtcc
     5761 gcaaggcgcc gcacgccgtc cgcgagagcc cgccggaggt cacgcggcgt ccgtccggcg
     5821 cggtcggccg cgggcagcgg agcgattccg atcggcgtca cctcggcgac gcggtacggc
     5881 gcgtcggtgt gcggctcgga ttcgatccgg tagcggaacg cgccgcgcag gagaaggttc
     5941 gagctcccgt cttccagcgg ctcgtggtga acgacctcgc ccgcgcagcc gatggaatac
     6001 accgcgggac gcgcgccgtc ggcggagtcg ccgggcttga gaagctgcat gccgatcagc
     6061 cggtccgagt cgagcgcgtc cgcgagcatc cggcggtagc gcggctcgaa gacgtgcagc
     6121 ggaatcgtcg ttccgggcag gaggacgacg ttcgggagcg gaaagaggcg gacgaggcgg
     6181 cgcccggaca tgaggtgaat gatgcatccg gaagggggct tcgcgcgcgg gccgtgttat
     6241 cttccgcgcc catggcgaac aaggacgacc gctacgcgga aaacgccccc ggcaagttct
     6301 acgtggacac ccagtgcatc gactgcgacg tgtgccgcgt gacggccccg aacaacttca
     6361 cccggcagga agagaagggc tactcctacg tgtcgaagca ggcttcgacg cccgaggaag
     6421 aggcccagtg ccaggaggcc atggactcct gcccggtcga ggcgatcggg aacgacgggg
     6481 aatgaccgaa gggggtccgg gggagagcgg aatctccccc ggcctgatca gtccagcgct
     6541 ctcctcgcgt ggtagctcga cctgacgagc ggccccgact ccacgactcc gaagcccatc
     6601 tcctcgcctt ccttccggat ctcctcgaac tcctgcggcg tgtagtaccg ctcgatgggc
     6661 agccttcgcg cgtgcggctg gaggtactgg ccgacggtca cgacgtcgac gcctgaggcg
     6721 cgcagatcgc ggagagtcgc cgagagttcc tctctcgtct cgccgagccc caccatgacc
     6781 ccgctcttca ctttcaaaga aatcttctct cggtctcttt tctctgcagc gcgcctcagc
     6841 agctcgagcg accgcgcgta gatcgcgtcg ggccggacgc ggcggtagag gcgctcgacg
     6901 gtctcggtgt tgtggttgag aacttccggc gcctcgtcga ggacggtccc cagcgcggcg
     6961 gcgctgccga gaaagtccgg gatgaggacc tcgaccgtga cgccgggcgc gagcgcccgg
     7021 atggcgcgga tcgtcgccgc gaagtgcgcg gcgcctccgt ccggcagctc gtcgcggttc
     7081 acggaggtca cgacggcgtg cgcgactccg agcgtccgca cggcctcggc gacgtgccgc
     7141 ggctcctcgg gatcgagggg ccggccgatt ccctgcgtga ccgcgcagaa cccgcagcgc
     7201 cgcgtgcaga cgtcgcccat gagcatgaac gtggccgtgc gctccacgcc ccagcactcc
     7261 gcgaggttcg ggcagcgcgc ctcctggcag acggtgttca ggctcttgtc ctcgacgagg
     7321 cgcgtgatct cggactcgaa cccggaggcc ggcatgcgca ccttgagcca ctcgggccgg
     7381 ggcgtgacgc cgtaacgggg gcgggagtcc gcgctcacgg agcgagtgta ccgggacgcg
     7441 ataggatcaa gggagaaacg atctcagcac aaggaggcct ccgatggtgt ccatcgcgtc
     7501 gctctggctg cccatcctgc tctccgccgc cgccgtgttc gtcgtgagct cgatcatcca
     7561 catgtttctc ggttaccact ccgccgatta ccggaagctg ccggccgagg acgacttcat
     7621 ggcggcggtc cgcaagctcg acatcccgcc cggcgattac atggtgccgt gcccggccag
     7681 ccacgccgcg ttcaaggatc cggccttcca ggagaagttc aaggccggtc cccgcgcgat
     7741 gatgaccgtt ttcggcccat acgaaggaat gggcgctcag ctcgcgcagt ggttcctgta
     7801 cgcgatcctc atcagcgtca ccgcggctta catctcgggg cgcgccctcg gccccggcgc
     7861 gcactacatg aaggtgttcc gcttcgcggg cgcgacggcg ttttacgggt acgcgctcgc
     7921 ccacctccag gcgtcgatct ggtacaagaa gtcgtggggg acgacgttcc ggaacgtctt
     7981 cgacggactc gtctacggcc tcgtgacggc cggattcttc ggctggctct ggccgcgcta
     8041 gggccgggtt ccccgttcga ccatctccct ctcgaacacc tcggcgaagt gagacacgaa
     8101 ccggccccct accgccccga gcggcggggg cggcgcgccg gacgtcttcg cgagggacgt
     8161 cacgccgccg tccgcgatgc cgcaggggac gaagagcgag aaccgcgcga ggtcggtcga
     8221 gacgttcagc gcgacgccgt gcgtcgtgat ccagcgcgaa aggtggacgc cgatggccgc
     8281 gagcttgtcg ttgcccaccc acacggatgc ccatctcgcc ggcgcggggc tccgcgccgc
     8341 ttcgacgccg aagtcgcgcg ccgtgcggat caggacctcc tcgagatcgc ggacgtagcg
     8401 gcgcacgtcc tgccggtcgg gggagaggtc caggaccggg tacgcgacga gctggcccgg
     8461 cccgtggaac gtgagctttc cgccgcggtc cgcgacgcgg agcgcgatgc ccttctgccg
     8521 gaggaaatcc tccgagacga ggagctcgcc gaggcccgcg tttcggccca ccgtgacgac
     8581 gtccgcgtgc tcgaggagga agagcgtctc cggcccgccc tctttcacgc gctcggcggc
     8641 ctgcttctgc agcgcgaagg ccgcgtccca cgagacgcgg ccttcgggga gcagggagac
     8701 gacgtcggtc cggcgcatgg cgcatgctac tgcgcagggt gagccgggtc acggaaggga
     8761 gcggaataac ggctccggct ggcgttccgg tgccgcccga cctaatgtac ccgtgccgac
     8821 ctggaggttg cccatgaaga gacgcctctg tctgacgatc ctcgcggccc tcacgctcgc
     8881 ctccgcggcg ccgcgcctcg gggcgcagga gcaggcccgg gagcccgagc cgtcggcccg
     8941 aacggcaccg ctcgcgggga ggtcgcctct gattttcgcg agcccgattc acgggggctg
     9001 ctacatcgct gcgccgggcc agtgcaagat ccacgtcgag ccgttcacga tcaacatctc
     9061 gtcggggacg aagttggcgc tgttcaagct cgtttcgatc cagctccccg gaaacgtcca
     9121 gacgacgatt tacgactggc ggcccgacca gtcgaacccg gcgcccgcgt ccggcaccac
     9181 gtacacgccg tccctcgtga cgcaggactt cgcggcgacg tgcgggaatt cctaccagat
     9241 cagccttcag gggagagaca ccggcgacgt caacacgttc aacctcggcc tcaccgggac
     9301 gttcacctgc ccgacgacgg tgccctgacg gcgatgaggc ctgtccgttc tgccgtcgtt
     9361 ctcgcggccg cgatcgccgc cggcgcggcg cggggcgagg agcgcgaggc cgcggtgcgt
     9421 tcgcccctga tcttcgcgag cccgatccac gccggctgca agacgtacaa cgcccggacg
     9481 tgccagatcg tggtcgagcc gttcacgatc aacctcgcgg cgggaaagaa gatggtctcg
     9541 ttccaggttt ccgtcgacgg ctcggtggtc tacgattggc ggccggacca gtccaacccc
     9601 gcgccggcgc tcgggtcgac gtacacgccc tcgcgggtcg ccctcggctt cggcgtcacg
     9661 tgcggcaaga gccacgtcgt gagcctccag ggccgcgaca cgggcgaccc gaactcgttc
     9721 aacctcggac agacgtctcc gatcacgtgc ccaccgcccg tgctgcagct gacgttctac
     9781 gcgctgcctc cctgtcgcct catcgacacg cggaaatcgt ccgggccgga cgccggcgcg
     9841 cccgtgctgt cggggggcga actgcgcgtc gtggggacgg cgcagaagtg cggcgttcca
     9901 ccgacggcgg cggctctctc gatgaacgtg acagtgaccg cgccgacgac cggcggcttc
     9961 ttcaccctct acgccgccga cgggacgttt ccggtcgcga gtcagctcag cttcggggcc
    10021 gggcagaccc gcgcgggcgc cacgatcgtc cccctcgcgc tcgacgcctc cggcttccgg
    10081 atcctgaacg gcgcggccgg gaccgctcac ctcatcgtgg acgtgaacgg gtacttcgag
    10141 tagcggccta ctccagctcg ggccaggtcc cctcttccag cgtcgacttc acgacgttca
    10201 cgaacgcgtc gccctcggag ccgtcgatga gacggtggtc gaaggtgagc gcgacgtatc
    10261 ccatcgtctt gatcgcgagc gagtcggagc cgtccggcat ctcgacgacc ttcgcgcgct
    10321 tctcgatcgt gccgacgccc atgatcgcga cctgcggctg gttgatgacg ggcgtcgcga
    10381 agagcgagcc gaacgacccg tggttcgtga ccgtgaacgt cccgccggcg acctcgtccg
    10441 gaaggagctt cttggcgcgc gcccgggccg cgaggtcgtt cgccgcacgc gcgaggccca
    10501 cgagcgagag gtcgtccgcg cgccggatga cgggcacgat caggccccag tccagcgcga
    10561 cggcgatccc gaggttcacc tccttgtgga agacgatgtc gtcgcccacg atcgacgcgt
    10621 tcacgagcgg gcgcgccttc agcgccgcga tcgccgcgcg gagcaggaac ggcaggtagg
    10681 tcagcttcgt gccgtactgc tcctcgaagc gcgccttcac gcggttgcgg agcttcgcga
    10741 tgccggaata gtcgacctcg aagaccgtcg tgacgtgcgc ggaggtctgc ttggactcga
    10801 ccatgcgctg cgcgatcttc cgccgcatcg cggacatggg ctcgatcgtg acgttgccgc
    10861 cggggaaggc gatcgtcgac gcgggccgcg cggcgagagg cgcctcgacg gggcgtgcgg
    10921 cgaggggagc gggagattcg gatttctgag aaggcgcggg cgagggcgcc gcgacgggcg
    10981 caggcggagc cgcggggccg gccgctctct ccaccttcaa agaatcttct ctcccggtcc
    11041 cggctcccgc caggaacgcc tcgatgtcct gcttcgtcac ccggccggag atgcccgtgc
    11101 cggggatctg cgagatgtcg acgcccttgt cctccgcgat cgcgcggacg acgggcgacg
    11161 agc
//
LOCUS       DAOMFY010000115        11115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1823_length_11115_cov_6.745841, whole genome shotgun sequence.
ACCESSION   DAOMFY010000115 DAOMFY010000000
VERSION     DAOMFY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_11115_cov_6.745841"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLB01_10645"
     CDS             complement(<1..239)
                     /locus_tag="PLB01_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77804.1"
                     /translation="MRRAPAAAAALLAAAACTFSSETTVSPHRLAKPLPGGLANLCVV
                     RPAAGAEEAFRDPAGTLRRALAAEPLLRQRTADARL"
     gene            complement(236..1954)
                     /locus_tag="PLB01_10650"
     CDS             complement(236..1954)
                     /locus_tag="PLB01_10650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQQ77805.1"
                     /translation="MPGSGASPTSVSASPATAAGGHRLLAAHARLAAIDPWSGRERLF
                     EELLAALLLGSGASRGVLLDPVQPSRSVASGLDVPPAALHALALRVAAAPRSTGPLTA
                     AELVPLFGGACTPFAIPVTAQEQTVAFVVLDAAPEDPAGFASATAHTAWLLDTVRMSE
                     KVQKADFELKYRVWELQSLYDVGLSIARTLDLESLADDVLMTSVSLLNARSGSLLVRA
                     QGDEGFFAKHVGEPLLHADAIYEVPAGAVLANTRESRPGSLKDAPAEKLLLVPIAVEN
                     RALGVLVVADKETRGGAVDDFTEADVRVASLFANQAAIALENARLHREAVEKEKMERE
                     MELAASIQKTILPDTLPEVTGLALAGANRPTKQVGGDYFDVYPLPGGLTALCVADVSG
                     KGVPAALLVSTVHACLHLLIPNLSGDLPALVARVNKHLVRFSSTRKFATLFVAVFDPA
                     TGLLRYVNAGHNPGLWLSGDGATLLPSGGVPVGMMPAAVHREAAVTLGPGDTLLLYSD
                     GITEALNRDDEEFGMDRLTELALEGRGSPPGELARRIFGAVSDFTAGVAQYDDQTVLI
                     ARVAGA"
     gene            complement(1926..2288)
                     /locus_tag="PLB01_10655"
     CDS             complement(1926..2288)
                     /locus_tag="PLB01_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQQ77806.1"
                     /translation="MSETLKVVVERPDAAVAILRTDGYINNTGGEEIAKEAYALLAGG
                     VTRLLLDLEKTKIVNSIGISILIEILEKLLDQGGKLAFCRLTPTIEKTFQIMGLAQYA
                     RIFPAPEPALAWLRGESD"
     gene            complement(2306..2743)
                     /locus_tag="PLB01_10660"
     CDS             complement(2306..2743)
                     /locus_tag="PLB01_10660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ77807.1"
                     /translation="MSTVERREVHLTIPVAPEMEIAATAQVSALGEWMEMGRDKIDEV
                     KMAVVEACINAFEHSATPDRKVALAFRIGSDDGGRGAYLEVDVADAGRGFDPTRVRTP
                     EIANALKSSRKRGWGIKIIESLMDDVRIESGEHGTRIVMRKYR"
     gene            complement(2767..2979)
                     /locus_tag="PLB01_10665"
     CDS             complement(2767..2979)
                     /locus_tag="PLB01_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77808.1"
                     /translation="MTSRNDTKAHASRSLDGVASLAPAVSSAPSAASRLLVPLLWAEL
                     AAFGALALSGKIPESLINALRALLTF"
     gene            complement(3261..4178)
                     /locus_tag="PLB01_10670"
     CDS             complement(3261..4178)
                     /locus_tag="PLB01_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77809.1"
                     /translation="MNRLETTCDVVVFCSSSGHCQLDCDYCVVHPVIKRNPSLTYEDL
                     AWFLGEVKHRTFLMFSGKGDFFAGYSKKDRLLDRLLDHDVEVALDVNGVLLQEYPEIA
                     PEKLRKIRHVNLTMHYRQIREKRVEKVWVENAKAIIERHTGELLLGTILSPLADDAWE
                     EALEFYEREIFRPTGQRMWMIRDCERPTSAAQEARLAALEQRFAPMIERSHTQDFAEA
                     FAGRSTVLCPAGQNYFRIWNDGRVEGCPYIPELADAGNLKERRLLRRDAHFRCSTPRF
                     CDCNNIEALGKMQYDTPATAGEPRPPAYV"
     gene            complement(4231..5736)
                     /locus_tag="PLB01_10675"
     CDS             complement(4231..5736)
                     /locus_tag="PLB01_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQQ77810.1"
                     /translation="MSVPRPPARDLEAFALKKKVLRLETLYDVSRSLTSASDERALLE
                     EILARAVPVLDAGRGFAAAFEDAAGAGAVASVGLERAPTPLDVASDPFVLDLARAKAP
                     LARAGETVLGRPAGSVAGVPLVSGNRVLGVLVVLEREARGGDAASFDEEDRRFLGSLA
                     ALAAPALEGSRRFRALAADLDRLREENRSLKGTTAVDELLVGDSPAMRRAKELIARAA
                     ASRVNVLITGESGTGKELAARLLHTGSSRRDGAFLALNCAAMPEGLLESELFGIEKGV
                     ATGVDGRPGKFELASGGTVFLDEIGDTPLAIQAKLLRVLQEREIERVGGRARIPVDVR
                     VLSATHQSLPDLIRQGRFREDLFYRLRVVEIAMPALRERREDIPRLAGHFLARIAARD
                     GRRPPALTRDAVKALLDYEFPGNVRELENLLEGAAALVSGDAIEAADLQFGIARGAAG
                     ALADDAKAAGVSLRHVENAHISRVLAQVKGNKSRAAKLLGVSRRTLYRKRV"
     gene            complement(5741..7186)
                     /locus_tag="PLB01_10680"
     CDS             complement(5741..7186)
                     /locus_tag="PLB01_10680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77811.1"
                     /translation="MAPRRVRVLPLVLALVGLAVAGTALAFRLRANRDVTTSSEQAYR
                     AYRSGVENDLKMYEREAMSSYAEALRYDPHFVMATLRLADKMRGRDPERAASLLASAA
                     RYRDDLTEREKLMLRIYEERWGKRDMKVLEALYDEYQKRFPKDPEGFQMRAGFLANSG
                     RMPEAIAEYERLITVNPNYAIAYNTLGYQAASRGENAKAEDYLKRYRFLAPNDANPLD
                     SLGELYAFTGRYDEAEENLRKAIAIKEDFYAAWGHLGSVEAGRGNPVKAAEYFRKASD
                     NAPNTYSRYDFRFLSAMCLVDAGRPDDAVKDLDAATAEIAAMAPGVEARKLKVGDAFR
                     RAGLLGRIGRTAEAEAALASVDLKEITDPKEPKSQEMAARQVSLIRGVIAMGAGRDAE
                     AVALLKASLEQKQDKGFASSDYYQNNAYVRLALATSLGRLGRPDEAEKALAPILEKNP
                     RFYPALLSLARARGQAPPAPIASVARAESAS"
     gene            complement(7251..9854)
                     /locus_tag="PLB01_10685"
     CDS             complement(7251..9854)
                     /locus_tag="PLB01_10685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQQ77812.1"
                     /translation="MSFSSPALLSVSGAVDAPRLTRRHLPLVAVGVMGALLALLSVVD
                     MFLPKAYDGVVPDPYSVGGILVRDLVPGGAAERAGLKSGDVILGIGHRMLNKATDAPP
                     ELLRHKVGEKVDYLVRRGGNVFETKVSLTPYRLGSATYYYFVFLGLLFFALGVFVVAR
                     RPDDSAVQVFYVLCILFMEFFVCRLRPSSYYWIDYVVQIAGTLALFLLPAVFLHFFLL
                     FPEKKTFRFADRRPGQPAAPPALEALQRFLNGSPLLLAILYSLPPVLYVLQMAGYRYG
                     GPRRLIYGAPTLNWILLADYLILGLLALAHTWWTNSDRAARRPILVLLLGTVAGIVPF
                     VVFAVFFPSLFREDRFLLWGVVPMALIPLTFAYAIVRFRLFDVQVIVRKSLVYAVLTA
                     VVTGMYALAVVAGNAIVSNVSSTTVFSSPAFAFGFGLVVVLLFDPLRRRMQKVVDRVF
                     FRDRADFQTALLEMSRSVVSQLERPKLRELLTSRTADLLRLERLELLLPRPEDGAFTE
                     PAAAAAGVQPLPMGAVLPQRLAERGAPIRLSDADDGGLDLASRRFVEAQRARGIGVVL
                     PLATHGKLLGFLAVGKKLSEEELSREDLDHLLTIANQGALGLEAAGLHEELTRRAEVE
                     RDLDIARDIQTSLFPRELPRVPGVEFFGVSRPARVVGGDFYDFLDVDSRPDGRLALVL
                     GDVSGKSIPASLLMVAAKEIVNARAMADPDPSAVFQASNRRLYEIKRRMFVSLSYFLL
                     DPRALAMTYAFAGQPTPLLVRPGSGLAAEIPSPASRLPLGAFRDTVYDAASLYLSRGD
                     LLLFYTDGLNEAMSAEMTPYGDERLKASLVRHARRSLPDLADELLEDVRQFTHGAEQY
                     DDITFVLMRVT"
     gene            9934..10593
                     /gene="tmk"
                     /locus_tag="PLB01_10690"
     CDS             9934..10593
                     /gene="tmk"
                     /locus_tag="PLB01_10690"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQQ77813.1"
                     /translation="MIRRRRSAPRGLFVTFEGIEGSGKTTQIARAAAWMEANGLPVLA
                     TRNPGGTELGKLLRGALLHSKGHIDAGAELLLMMADRRHHLQTMIEPALAKGTHVLCD
                     RYTDASRAYQGAGRGLGEKKVDELHERWCRRDPDRTYLFDLPVETALARVAKRRGASY
                     DRFEDEDRDFHERVRRAYRRRARMEPKRFVVIEASRSADDVFSSLEENLLLLFKLRGG
                     R"
     gene            10590..>11115
                     /locus_tag="PLB01_10695"
     CDS             10590..>11115
                     /locus_tag="PLB01_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77814.1"
                     /translation="MSGAPVVLLAGPGSKRLEAAALEEAAATICGRGFDPESNAARRV
                     FRREHPDLMVAAPERRRRVNAPHFEEGDTKETSIPTALVRAVAADSTRLPYEAPMRAV
                     VFLDVDRTEPAAFSALLKILEEPPKKTRFLLTATRPRLLPPTILSRVTLQALPGSTRA
                     GTIDALVARGMARED"
BASE COUNT         1743 a         3764 c         4039 g         1569 t
ORIGIN      
        1 agacgtgcgt cagccgttcg ctgccgcaac agtggttccg cggccagcgc gcgccgcagg
       61 gtgccagccg gatccctgaa cgcctcttcc gcgccggccg ccggccgtac cacacacagg
      121 ttggcgaggc cgcccggcag cggcttggcg agccggtgcg gggacacggt cgtctcactg
      181 gagaaggtgc aggccgcggc cgcgagcagg gccgccgcag cggccggcgc gcgcctcacg
      241 cgcccgcgac gcgggcgatg agcaccgtct ggtcgtcgta ctgcgcgacg cccgcggtga
      301 agtcgctcac ggccccgaag atgcgacgcg ccagctcgcc gggaggcgac ccgcgccctt
      361 ccagcgcgag ctccgtgaga cggtccatcc cgaattcctc gtcgtcgcgg ttgagcgcct
      421 ccgtgattcc gtccgagtag agaagcagcg tgtcgccggg cccgagggtc acggcggctt
      481 cgcggtggac ggcggccggc atcattccga cgggaacccc gccgctcggc aggagcgtcg
      541 cgccgtcgcc ggagagccag aggccggggt tgtgtccggc gttcacgtag cgcagcagac
      601 ccgtcgccgg atcgaacacc gcgacgaaga gcgtcgcgaa cttgcgcgtc gaggagaagc
      661 gcacgaggtg cttgttcacg cgggcgacga gtgccgggag gtcgcccgag aggttcggga
      721 tgagaaggtg gaggcaggcg tggaccgtgg acacgaggag cgccgccggg acgcccttgc
      781 ccgaaacgtc cgccacgcac agcgccgtga gcccgccggg cagcgggtag acgtcgaagt
      841 agtcgccgcc gacctgtttc gtcgggcggt tggcgcccgc gagggcgagg cccgtgacct
      901 cggggagcgt gtcgggaaga atcgtcttct ggatggaagc ggcgagctcc atctcgcgtt
      961 ccatcttttc cttctcgacc gcctcgcggt ggaggcgcgc gttctcgagc gcgatcgccg
     1021 cctggttcgc gaagagactc gcgaccctca cgtcggcctc cgtgaagtcg tccaccgcac
     1081 ccccgcgcgt ctccttgtcg gcgacgacga ggacgccgag cgcgcggttc tcgaccgcga
     1141 tcggcacgag gaggagcttc tcggcaggcg cgtccttcag ggagccgggc cggctctcgc
     1201 gcgtgttcgc gaggacggcc cccgcgggca cttcgtagat cgcgtccgcg tggaggagcg
     1261 gctcgcccac gtgcttggcg aaaaagccct cgtcgccctg cgcgcgcacg aggagcgacc
     1321 ccgagcgcgc gttcaggagc gacacggacg tcatgaggac gtcgtcggcg agggactcga
     1381 ggtcgagcgt ccgggcgatg gagagcccga cgtcgtagag gctctggagc tcccagacgc
     1441 ggtacttcag ctcgaagtcg gccttctgga ccttctcgga catccgcacc gtgtcgagga
     1501 gccaggccgt gtgcgccgtc gcggacgcaa agcccgcggg atcctcgggc gccgcgtcga
     1561 gaacgacgaa cgcgacggtc tgctcctgtg ccgtgacggg aatcgcgaag ggcgtgcacg
     1621 cgcctccgaa gagggggacg agttcggccg ccgtcagggg cccggtcgag cggggcgcgg
     1681 ccgcaacccg gagcgcgagc gcgtgcaggg cggccggagg cacatccagg ccgctggcca
     1741 ccgagcggga gggctggacc gggtcgagca ggacgccgcg cgaggccccc gagccgagga
     1801 ggagcgcagc gagaagctcc tcgaagaggc gctcgcgtcc gctccaggga tcgatcgccg
     1861 cgagccgggc gtgcgccgcc aggaggcggt ggccgcccgc ggccgtcgcc ggcgaggcgg
     1921 aaacgctagt cggactcgcc ccggagccag gcaagggcgg gctcgggagc ggggaagatg
     1981 cgtgcatatt gcgccagacc catgatctgg aacgtcttct cgatcgtcgg cgtcaggcgg
     2041 cagaaggcga gcttccctcc ctggtcgagc agcttctcga ggatctcgat gaggatcgag
     2101 atgccgatcg agttcacgat cttcgttttc tcgaggtcga ggagaaggcg cgtcacgccg
     2161 cccgcgagaa gagcgtacgc ctccttcgcg atctcttcgc cgccggtgtt gttgatgtag
     2221 ccgtcggtcc ggaggatcgc gacggccgcg tccggccgct ccacgacgac tttcagggtc
     2281 tcgctcattc ggctcccttt atatcctacc ggtacttccg catcacgatg cgcgtcccgt
     2341 gctccccgct ctcgatgcgg acgtcgtcca tcaggctctc gatgatcttg atgccccacc
     2401 cgcgcttgcg gctggacttc agggcgttcg cgatctcggg ggtcctgacg cgcgtggggt
     2461 cgaagccccg gccggcgtcg gcgacgtcca cctcgagata ggcgccgcgg ccgccgtcgt
     2521 ccgagccgat cctgaacgcc agggccacct tgcggtccgg cgtcgcgctg tgctcgaacg
     2581 cgttgatgca ggcctcgacg acggccatct tgacctcgtc gatcttgtcc cggcccattt
     2641 ccatccattc gccgagcgca gaaacctgcg ccgtcgccgc gatctccatc tccggagcaa
     2701 ccgggatggt tagatggact tcacgcctct cgacggtcga catcgcggac ggcagttcct
     2761 cactcttcaa aacgtcagga gcgcgcgaag ggcgtttatc agggactccg gaatcttacc
     2821 cgagagggcg agagcgccga acgcggcgag ctcggcccag aggagcggca cgagcaggcg
     2881 cgaggcggcg gaaggcgcgg acgacacggc aggcgcgagc gaggcgacgc cgtccaagga
     2941 tcgcgaggcg tgggcctttg tgtcgttgcg gctcgtcatg ggctggctcc ggttagcccc
     3001 aatacagcaa gcgacgcgcc agccccgtta gcgtcgcgaa tgcggcgctg aagcgaagtt
     3061 aagttattga aagagaaaga tttctaagaa ggtgatcgga acgctgcgca aaggcaagga
     3121 ttgcgccact atgtcacatt aacagcgacc agcttagaat aggcctatta accattctaa
     3181 gaaaactact taacgtgagt gagcgaaaat gtcacatcgt ggcgcattgt caccacaata
     3241 agggggagca gagcagcact tcagacgtac gccggaggcc ggggctcccc tgccgtcgca
     3301 ggcgtgtcgt actgcatctt cccgagggct tcgatgttgt tgcagtcgca gaaccgcggc
     3361 gtcgagcagc ggaagtgggc gtcgcgacgc aggaggcgcc gctccttcag gtttccggcg
     3421 tccgccagct ccgggatgta gggacagccc tccacgcgcc cgtcgttcca gatccggaag
     3481 tagttctgcc ccgccggaca gagcacggtc gagcgccccg cgaacgcctc cgcgaaatcc
     3541 tgggtgtggg agcgctcgat catcggcgcg aagcgctgtt cgagcgcggc gagcctcgcc
     3601 tcctgcgccg ccgaggtcgg ccgctcgcag tcccggatca tccacatccg ctggcccgtc
     3661 gggcggaaga tctcccgctc gtagaactcg agggcctcct cccacgcgtc gtcggccagg
     3721 ggcgagagga tggtcccgag cagcagctct cccgtgtgcc gctcgatgat cgccttcgcg
     3781 ttctcgaccc agaccttctc gacgcgtttc tcccggatct gccggtagtg catcgtcagg
     3841 ttgacgtgcc ggatcttccg aagcttctcc ggcgcgatct ccgggtactc ctgcaggaga
     3901 acgccgttga cgtcgagcgc gacctcgacg tcgtggtcga gcagccggtc gagaagccgg
     3961 tccttcttcg agtaccccgc gaagaagtcc cccttccccg agaacatgag gaacgtccgg
     4021 tgcttcacct cgcccaggaa ccacgcgaga tcctcgtagg tgagggacgg gttccgtttg
     4081 atgacgggat gcacgacgca gtagtcgcag tcgagctggc agtgcccgga cgacgagcag
     4141 aaaacgacga cgtcgcaggt cgtctcgagg cggttcatcg agggctcctc gtcacgcgcc
     4201 gaaatgctag caccgcgcgg acaccgcgac tcacactcgt ttgcggtaga gcgtccgacg
     4261 gctgacgccg aggagcttgg ccgcgcggct cttgttgccc ttcacctgcg cgaggacgcg
     4321 gctgatgtgc gcgttctcca cgtgcctcag gctgacgccc gcggccttcg cgtcgtccgc
     4381 gagggcgccc gcggcgccgc gcgcgattcc gaactgcagg tcggccgcct cgatcgcgtc
     4441 gcccgagacg agcgccgcgg cgccctcgag caggttctcg agctcgcgca cgttgccggg
     4501 aaactcgtag tcgaggagcg ccttcacggc gtcgcgcgtg agcgcgggcg gccggcgccc
     4561 gtcgcgcgcc gcgatgcggg cgaggaagtg gcccgcgagc cgcgggatgt cctcgcgccg
     4621 ctcgcgcagc gcgggcatcg cgatctcgac gacgcgcagg cggtagaaga ggtcctcgcg
     4681 gaaccggccc tggcggatga ggtccggcag gctctggtgc gtcgcggaca ggacgcgcac
     4741 gtccacgggg atgcgcgcac gcccgcccac gcgctcgatc tcgcgctcct ggaggacgcg
     4801 caggagcttg gcctggatcg cgagcggcgt gtcgccgatc tcgtccagga acacggtgcc
     4861 gccgctcgcg agctcgaact tccccggccg cccgtccacg cccgtcgcga cgcccttctc
     4921 gatgccgaac agctcgctct cgaggagtcc ctcgggcatc gcggcgcagt tcagggcgag
     4981 gaaggctccg tcccggcgcg aggatcccgt gtgcaggagt cgcgcggcga gctccttacc
     5041 ggtgccgctc tcccccgtga tgaggacgtt gacgcgggag gccgcggcgc gcgcgatgag
     5101 ctccttggcg cggcgcatgg cgggcgagtc gccgacgagg agctcgtcca cggcggtcgt
     5161 gcctttcaac gagcggttct cctcgcgcaa gcggtcgaga tccgcggcga gcgcccggaa
     5221 gcggcggctt ccctccagcg caggggccgc gagcgccgcg agcgagccga gaaaccggcg
     5281 gtcttcctcg tcgaaggagg ccgcgtcgcc gccgcgcgcc tcgcgctcga ggacgacgag
     5341 gacgccgagt acgcgattgc cggaaacgag aggcacgccc gcgacgctgc cggcgggacg
     5401 gccgaggacg gtctcccccg cccgggcgag cggcgccttc gcgcgcgcga gatcgagaac
     5461 gaacgggtcg gacgcgacgt cgagaggcgt cggcgccctt tcgaggccga cggaggcgac
     5521 ggcgcccgcg cccgcggcgt cctcgaacgc cgccgcgaag ccgcggcccg cgtcgaggac
     5581 gggcacggcc cgcgcgagga tctcctcgag gagcgcgcgc tcgtcggagg ccgacgtgag
     5641 cgagcggctc acgtcgtaga gcgtctcgag acgcaggacc ttcttcttga gcgcgaaggc
     5701 ctcgaggtcg cgcgcgggcg ggcgcgggac gctcacgagg tcaggacgcg ctctccgcgc
     5761 gggccaccga tgcgatcggg gcgggcggcg cctgcccgcg ggcgcgcgcg agcgacagca
     5821 gcgcggggta gaagcggggg ttcttctcga ggatcggtgc gagcgccttc tccgcttcgt
     5881 ccgggcggcc gagacggccg agcgacgtcg cgagcgcgag acgcacgtag gcgttgttct
     5941 ggtagtagtc cgaggacgcg aaacccttgt cctgcttctg ctcgagcgag gccttgagga
     6001 gcgccaccgc ctcggcgtcg cgcccggctc ccatcgcgat cacgccgcgg atgaggctca
     6061 cctggcgggc ggccatctcc tgggacttcg gctccttcgg gtccgtgatc tccttgagat
     6121 ccacggacgc gagagccgcc tcggcctcgg ccgtgcggcc gatgcgcccg aggaggcccg
     6181 cgcgccggaa cgcgtcgccc accttcagct tgcgagcctc cacccccggc gccatcgcgg
     6241 cgatctcggc cgtggcggcg tcgaggtcct tcacggcgtc gtccggacgg cccgcgtcca
     6301 cgaggcacat cgcggacagg aagcggaagt cgtaccggga gtaggtgttc ggggcgttgt
     6361 cgctggcctt gcggaagtac tcggccgcct tcaccgggtt cccgcggccg gcctcgacgg
     6421 agccgaggtg gccccatgcg gcgtagaagt cttccttgat cgcgatcgcc ttgcgcaggt
     6481 tctcctcggc ctcgtcgtac cggcccgtga acgcgtagag ctcgccgagc gagtccagcg
     6541 ggttcgcgtc gttcggggcg aggaaccggt agcgcttgag gtagtcctcg gccttcgcgt
     6601 tctcgccccg gctcgcggcc tgatagccca gggtgttgta ggcgatcgcg tagttcggat
     6661 tcacagtgat gaggcgctcg tactcggcga tcgcctcggg catgcggccg ctgttcgcga
     6721 ggaagccggc gcgcatctgg aagccctcgg ggtccttcgg aaagcgcttc tggtactcgt
     6781 cgtagagggc ctcgagcacc ttcatgtctc gcttgcccca gcgctcctcg tagatgcgaa
     6841 gcatgagctt ttcgcgctcc gtgaggtcgt cgcggtaccg ggccgcgctc gcgaggagcg
     6901 acgcggcgcg ctcggggtcg cgcccgcgca tcttgtcggc gagccggagc gtcgccatca
     6961 cgaagtgcgg gtcgtaccgg agggcctcgg cgtacgagga catcgcctcg cgctcgtaca
     7021 tcttgaggtc gttctcgacg cccgagcggt aggcccggta agcctgctcc gacgacgtcg
     7081 tgacgtcccg gttcgccctc agccggaacg ccagggccgt gcccgcgacg gccaggccga
     7141 cgagggccag gacgagcggc aggacgcgga cgcggcgagg ggccatggtt cctccgggga
     7201 ccccgccggg gggccctttc ccatgatacg cgccggcggc gggttggttt tcaggtcacc
     7261 cgcatcagga cgaacgtgat gtcgtcgtac tgctcggccc cgtgcgtgaa ctgccggacg
     7321 tcctccagga gctcgtcggc cagatcgggc agggaccggc gggcgtggcg cacgagcgac
     7381 gccttgaggc gctcgtcccc gtagggtgtc atttcggccg acatcgcctc gttcaggcca
     7441 tccgtgtaga agagcaggag gtccccccgg gacaggtaca gggacgccgc gtcgtagacc
     7501 gtgtcccgga aggcgccgag cggcaggcgc gaggccgggg acgggatctc ggccgcgagc
     7561 ccggagcccg ggcgcacgag aagcggcgtc ggctggcccg cgaacgcgta cgtcatcgcg
     7621 agcgccctcg ggtcgaggag gaaatacgag agcgacacga acatgcgccg cttgatctcg
     7681 tagagccgcc ggttcgatgc ctggaagacc gcggacggat ccgggtcggc catcgcgcgc
     7741 gcgttcacga tctccttggc cgcgaccatg aggagcgacg cggggatcga cttgcccgag
     7801 acgtcgccga ggacgagcgc gaggcggccg tccggacggg agtccacgtc gaggaagtcg
     7861 tagaaatccc cgccgacgac gcgcgccggc cggctgacgc cgaagaactc gacgccgggg
     7921 acgcgcggga gctcgcgcgg gaagaggctc gtctggatgt cgcgcgcgat gtcgaggtcg
     7981 cgctcgacct cggcccggcg cgtgagttcc tcgtggaggc ccgcggcctc gaggccgagg
     8041 gcaccctggt tcgcgatcgt gaggaggtga tcgaggtcct cgcgcgagag ctcctcctcg
     8101 gagagcttct ttccgaccgc gaggaagccg aggagcttgc cgtgcgtggc gagcgggagg
     8161 acgacgccga ttccgcgcgc gcgctgcgcc tccacgaagc gccgcgacgc aaggtcgagt
     8221 cctccgtcgt cggcgtccga gagacggatc ggcgccccgc gctcggccag cctctgcggc
     8281 aggaccgcgc ccatgggcag cggctggacg cccgccgccg ccgccgccgg ctcggtgaac
     8341 gcgccgtctt ccggccgcgg gaggaggagc tcgaggcgct cgagccgcag gagatcggcc
     8401 gtccgcgacg tgagcagctc ccggagcttc gggcgctcga gctgcgagac gaccgagcgg
     8461 ctcatctcga ggagcgccgt ctggaagtcg gcgcggtcgc ggaagaatac gcggtcgacg
     8521 accttctgca tccggcggcg caacgggtcg aagaggagga cgacgacgag gccgaagccg
     8581 aacgcgaagg ccggcgacga gaagaccgtc gtggaggaga cgttcgagac gatcgcgttg
     8641 cccgcgacga ccgcgagcgc gtacatcccc gtcacgacgg ccgtgaggac cgcgtagacg
     8701 agggacttgc gcacgatgac ctgcacgtcg aagagccgga accgcacgat cgcgtacgcg
     8761 aacgtgagcg ggatgagcgc catcggcacg acgccccaca ggaggaagcg gtcctcgcgg
     8821 aagagcgacg ggaagaagac ggcgaagacg acgaacggga cgatccccgc gaccgtcccg
     8881 aggagcagga cgaggatcgg ccggcgcgcc gcgcggtcgg agttcgtcca ccacgtgtgc
     8941 gcgagcgcga ggagtccgag gatgaggtag tccgcgagga ggatccagtt cagcgtcggc
     9001 gcgccgtaga tgaggcggcg cggccctccg taccggtagc cggccatctg gaggacgtac
     9061 aggaccggcg gaagcgagta gaggatcgcg aggagcagcg gcgacccgtt caggaaccgc
     9121 tggagcgcct cgagcgcggg cggggccgcc ggctggccgg ggcgccggtc cgcgaaccgg
     9181 aacgtcttct tctcggggaa gaggaggaag aagtggagga agacggccgg caggaggaac
     9241 agcgcgagcg tgccggcgat ctggacgacg tagtcgatcc agtagtagct cgacgggcgc
     9301 agccggcaga cgaagaactc catgaagagg atgcagagga cgtagaagac ctgcaccgcg
     9361 gagtcgtccg gccggcgcgc gacgacgaag acgcccagcg cgaagaagag gaggccgagg
     9421 aagacgaagt agtagtaggt ggccgagccg agccggtacg gcgtcaggga gaccttcgtc
     9481 tcgaacacgt tcccgccgcg gcggacgagg tagtcgacct tctccccgac cttgtgccgg
     9541 aggagctccg ggggagcgtc ggtcgccttg ttgagcatcc ggtggccgat cccgaggatc
     9601 acgtcgccgg acttcaggcc ggcccgctcc gccgcgcctc cggggacgag gtcgcgcacg
     9661 aggatgccgc ccacggagta agggtcgggc acgacgccgt cgtaggcctt cggcaggaac
     9721 atgtccacga ccgacaggag ggcgagcagc gcccccatga ctccgacggc gacgagcggc
     9781 aggtgccggc gcgtgaggcg cggggcgtcg acggcgcccg agacgctgag gagagccggc
     9841 gacgaaaacg acattactgg acccagtata cgaacggcgt gccagcgacg ttcccgggac
     9901 ggatccgccc cggcgcgtgt gagaatccgc gccgtgatcc gccgacgccg gtccgccccg
     9961 cggggccttt tcgtcacctt cgagggcatc gagggttccg gaaagaccac ccagatcgcc
    10021 cgggcggccg cgtggatgga ggcgaacggc ctgcccgttc tcgcgacccg gaaccccggc
    10081 ggcaccgagc tcgggaagct cctccggggg gccctcctcc actcgaaggg ccacatcgac
    10141 gcgggggccg agctcctcct catgatggcc gaccggcgcc accacctcca gacgatgatc
    10201 gagccggcgc tcgccaaagg gacgcacgtc ctctgcgacc gctacacgga cgcctcgcgc
    10261 gcgtaccagg gggccggccg ggggctcggc gaaaagaaag tcgacgagct ccacgaaaga
    10321 tggtgccgcc gcgacccgga ccggacgtac ctcttcgacc tgcccgtcga gacggcgctc
    10381 gcgcgcgtcg cgaagcgacg cggcgcgtcg tatgaccgat tcgaagacga agacagagat
    10441 ttccatgaaa gagtgcgcag ggcctaccgc cggcgcgccc gaatggagcc gaagcgcttc
    10501 gtcgtaatcg aggcctcccg ctccgcggac gacgtcttct cttcactcga agaaaatctt
    10561 cttcttctct tcaaactccg ggggggccgg tgagcggcgc gcccgtcgtc ctcctcgcgg
    10621 gcccgggttc gaagcgcctc gaggccgccg cgctcgagga ggccgccgcg acgatctgcg
    10681 ggcgcggctt cgatcccgag tcgaacgcgg cgcgccgcgt cttccgccgg gagcatcccg
    10741 acctcatggt cgccgcgccc gagcggcggc ggcgcgtgaa cgcgcctcac ttcgaagagg
    10801 gcgacacgaa ggagacgagc atcccgacgg ccctcgtgcg ggcggtcgcc gccgactcca
    10861 cgcgcctccc ttacgaggcg ccgatgcgcg ccgtcgtctt cctcgacgtg gaccgcaccg
    10921 agcccgcggc gttcagcgcg ctcctgaaga tcctcgagga gccgccgaag aagacgcgct
    10981 tcctcctgac ggcgacgcgg ccgcgccttc tgccgccgac gatcctttcg cgcgtcacgc
    11041 tgcaggcgct gcccgggtcg acgcgcgcgg ggacgatcga cgcgctcgtc gcgcgcggga
    11101 tggcccgcga ggacg
//
LOCUS       DAOMFY010000116        11072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1835_length_11072_cov_8.290188, whole genome shotgun sequence.
ACCESSION   DAOMFY010000116 DAOMFY010000000
VERSION     DAOMFY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_11072_cov_8.290188"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..967)
                     /locus_tag="PLB01_10700"
     CDS             complement(386..967)
                     /locus_tag="PLB01_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQQ77815.1"
                     /translation="MLDSPVLVLNKGFTALTLTDVKRTFTLFYKGHVRAVLPDYTTYA
                     WDEWQDIPVQADDDVVTTPSLVLKVPRVVQLLSCDRSPRHEVKFSRHNIYMRDGNRCQ
                     YCGRRFSTSDLSLDHIVPLSRGGPSNWENIVCACLPCNVKKGNRLPDEAGMKLRRPAA
                     KPKATPLGLMSPHRIHPTWKNFLDMAYWNVELK"
     gene            1008..2039
                     /gene="waaC"
                     /locus_tag="PLB01_10705"
     CDS             1008..2039
                     /gene="waaC"
                     /locus_tag="PLB01_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HQQ77816.1"
                     /translation="MRILFVRLSSFGDVLFALPAAKALRASLADAHLTWAIEPPLAPL
                     VEGAAYVDDVIGVDTRGWRRRGFGKETREEISEFLRRVRGADFDLVVDAQGLFKSALV
                     TAAARSGRKVGFGFRVATEWTNCLVTDERVDVDRAARPHVLDQMLALAEHVTGKSGFE
                     RTPDVTHLVERPDPVVDSWLEQTGGRPFAVLQPFSSKVNKEWGADDVVAFAAWLVSEG
                     IQPVLRWGPGEESRAAELIDASKKSSLLPSSLSLPLLLAPPTNPASSARLAARAALFV
                     GADTGPTHLAAAAGTPTLALFGPTPPERFGPVGPRVAVLRESSAAYNRSGGRWPVEGV
                     REAASRLLA"
     gene            2011..2850
                     /locus_tag="PLB01_10710"
     CDS             2011..2850
                     /locus_tag="PLB01_10710"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQQ77817.1"
                     /translation="MKPRADSSRRRGRAELVLAAGALAAAAFLVPARRSGADRPPVVV
                     AARETLPKDRLFAVPFGEGETLVYTIAWLKIEGGGMTLRTARETTSDGVPMHRITLTA
                     TSNDYVSKFYPVRDLYETWVDARDFQPLRFEKHAREGRYESDEVEEFDLAKKIGSWRE
                     DRTPLPDRIQDIISSFYFLRAQPLAVGSDVRVDMFSRGKIYKLKAAVLEKERVETEAG
                     VFDAFKVQPQLRENETAEDRNRGKLFLWFSDDDRRLPVMAKTVMPIGSVTARLTKVVA
                     GTR"
     gene            2861..4588
                     /gene="pilB"
                     /locus_tag="PLB01_10715"
     CDS             2861..4588
                     /gene="pilB"
                     /locus_tag="PLB01_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HQQ77818.1"
                     /translation="MAVKLGDLLVKAKLVTPEQIQEALVEVQASGMKLGEALVKLGYV
                     SEDDITETLSAQFGVPSINLSHFEIDPNVLKLVAPEVARKYNILPVNKTGATITIAMA
                     DPTNVFAMDDIKFMTGYNVEPVVASEIALQQAIDRHYGATHALELKKVMEEMSVVDTA
                     DTSLEVVEESEQETIDLEKLVDEGEEAPVIRLVNIILTDAIKRSASDIHIEPYEREYR
                     VRYRIDGILYEVMNPPPKLKEAIASRIKILAKLDIAEKRLPQDGRIKIKMKLAGKVKE
                     LDYRVSVLPTLFGEKIVCRLLDKDNLMLDMTKLGFEKDSLLRFEKAILRPWGMVLVTG
                     PTGSGKTNTLYSALSRINTPETNIMTAEDPVEFNLPGINQVQMKDAIGLNFAAALRSF
                     LRQDPNIILVGEIRDFETAEIAVKASLTGHLVLSTLHTNDAPSTINRLMNMGIEPYLV
                     ATSVVLIAAQRLIRRVCASCKAPTEVAPQVLVSLGFTPEDAKTVQVMKGKGCEKCNNT
                     GYKGRVALVEVLEITEEVRELILSGASSIEIKRKGLEEGMISLRRSGLQKIKDGLTSI
                     EEVVRETVL"
     gene            4591..5334
                     /locus_tag="PLB01_10720"
     CDS             4591..5334
                     /locus_tag="PLB01_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQQ77819.1"
                     /translation="MGARPEEVYFFGTCVVDLFFPEAGLAGIELLAREGVRVVFPRAQ
                     TCCAQPAFNCGHHEEARAVARAQMALFPKAIPVVVPSGSCAGMMKHHYPELFSGTPDH
                     EAAVAFADRVHELTQFLAGDLGVSLTDRGPPVAVTWHASCHGLRELGLDAEPKALLAQ
                     LANVTVVPLARERECCGFGGTFSVRYPDVSSALVADKAVDVEATGAPVVLSADAACLL
                     NIGGALDARGTAVAARHLAEFLWERTRAR"
     gene            5324..6769
                     /locus_tag="PLB01_10725"
     CDS             5324..6769
                     /locus_tag="PLB01_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HQQ77820.1"
                     /translation="MRADDETPASPDFHAASSRALADEGLRANFRRAMDGLVGKRAAQ
                     FPDSAEWRTLRARGAEIRARALARLPELLERLEQKCAKNGIFVHWASTTEEANGIVLE
                     ILRRNGATRLVKGKSMVSEEMHLNEFLEENGVESLETDLGEYVIQLDGETPSHIIMPA
                     IHKNRQQIARLFGQKIRDAVYTEDVDALTAIARRVLRQKFLEAEAGLSGVNFAVAETG
                     TLCLVENEGNGRMSTHVPPVHIAVMGLEKVVEKLEDVPPLLTLLTRSATGQPITTYVN
                     MISGPRGPLEKDGPREVHLVLLDAGRSRIFADPELRATLRCIRCGACMNHCPVYSRVG
                     GHAYEAVYPGPIGQVLTPQSEGLGRRHDLPDASSLCHACTEVCPVEIPISKLLVRLRA
                     EAAQPPAGSNVLGAGGGRSRLEDLAWGAWKAAHVSPLLHRAATRVFRLVHPLIPSWLG
                     PMRGWTKSRTKPRPAARTLHELVREKGLPDA"
     gene            6915..7430
                     /locus_tag="PLB01_10730"
     CDS             6915..7430
                     /locus_tag="PLB01_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HQQ77821.1"
                     /translation="MEAARTEFLEATEAAWPEKLNGFLLAEGLRRVAFPPATDAGRRL
                     TAACPAGGDVTLVPYDRPVEEMKDALFSEIDAGFTTARGAIAETGSLVLWPSPEEPRL
                     LSLVPPIHIALLDRRTIRDTFEDVLREERWAEGIPPNALLISGPSKTADIEQTLAYGV
                     HGPKRLVVVLV"
     gene            7490..7696
                     /locus_tag="PLB01_10735"
     CDS             7490..7696
                     /locus_tag="PLB01_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HQQ77822.1"
                     /translation="MRTTLNIDDAALQAALAADPGKTKTAVINAALRDYARRRALRGL
                     RRLAGKAGWSGDLDALRKRRPRAS"
     gene            7693..8091
                     /locus_tag="PLB01_10740"
     CDS             7693..8091
                     /locus_tag="PLB01_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HQQ77823.1"
                     /translation="MRVLVDTSAWAEFLNGTASPEARAVDRLLAGEDDVCTCGLVVAE
                     VFQGIRRDREEVEAFFRKMTFLEPSGIDVYLKAADVHRTLRRRGVTIRSTIDCFLAVL
                     AAENGCALLFRDRDLDRIVASGLLDLPARA"
     gene            complement(8098..9942)
                     /gene="ftsH"
                     /locus_tag="PLB01_10745"
     CDS             complement(8098..9942)
                     /gene="ftsH"
                     /locus_tag="PLB01_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQQ77824.1"
                     /translation="MLKSTRFNVTYLLLAVVGVFLLHDAWTASRQVAAIPYSEFQRLL
                     REKKVKEVVVTADRIEGELTAPENGRSRFVATRVEPGLARELDAHGIKYAARAEETLL
                     PTLLSWVVPVLIFFGAWILLGRFLAGRMGGGSGGLLSIGKSKAKVYVETDTKVTFQDV
                     AGVDEAKAELAETVSFLKDPGSYGRLGARAPKGILLVGPPGTGKTLLARAVAGEAGVP
                     FFSISGSEFVEMFVGVGAARVRDLFEQARLKAPCIIFIDELDALGKARNSQPGFGGHD
                     EKEQTLNQLLVELDGFDPRSGIVLLAATNRPEILDPALLRAGRFDRQVLVDRPDKRGR
                     ADILKVHAARVKVRADLNLEEVAALTPGFTGADLANLVNEAALAATRRNATEVSREDF
                     TAAIERIVAGLERKNRLLNDAERTIVAHHEMGHALVAAALPGMDVVHKVSIIPRGIGA
                     LGYTIQRPTDDRYLMTRAELEAKMAVLLGGRAAEKIVFDHLSTGAADDLAKATSIARA
                     MVTRYGMVPELGHVAYDDDGGSFLAPGGPLAHGRRLVGDETNREIDRAVRELLAAAYE
                     RASGILRTNRPLLEEGARRLLDEETLDDAQLAALFARVRRADGDREAA"
     gene            complement(9955..10086)
                     /locus_tag="PLB01_10750"
     CDS             complement(9955..10086)
                     /locus_tag="PLB01_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77825.1"
                     /translation="MVGPGIGFFAIAASLVVIAAILGISIVLSLVRPPKPAAVPSGS"
     gene            complement(10080..10976)
                     /locus_tag="PLB01_10755"
     CDS             complement(10080..10976)
                     /locus_tag="PLB01_10755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQQ77826.1"
                     /translation="MKNQLKTVVLLGSLSAIIVGLGALVAPGSLALFGALALAVNLGA
                     YFFSDRIVLRMHHAREAGADEAPELHAIVEELSARAGIPRPRVFLIPEEQPNAFATGR
                     NPERGVVAVTEGLVRLLDRRELRGVIAHELAHIKNRDILVSSLAAAAASLVTYVAQAV
                     SFGAFFGGSEEEESEGGAAGGLLAAVVAPLAATLIQFGISRAREFMADETGARISGEP
                     EALARALVKLEQASHLVPADAAPATASLFIVNPFGAVESLSRWFSTHPRTEERVERLL
                     AMAGRAPLDAGSPLLPRLLPEW"
BASE COUNT         1729 a         3893 c         3751 g         1699 t
ORIGIN      
        1 ggcttcgcgg tggccttctt ggcctcttcc gcgaacgcgg cacccaggga caggacggcg
       61 acggcgacga gagcggaaag cgtcttcttc atttcttcct ccgttttccc ctttcatctg
      121 tggccggccc ggggtctgcc gacagaaccc ccagcgcaac cctgctgcca ccgctccgag
      181 gcgggttcga agagaacgct aaagagattt cctagaatgg tttaggagga cgaggcgacg
      241 ggaggcggcc gcgcgcgccg agggggcagt gcacgtttcc gtgcattggc tgaactctaa
      301 gcgtgggatt tccgcgagcc ggtcgtcctc gccccgcccc tcttcacctt ctaagaaatc
      361 ccctcttctc tcttcgcttc gtcttctact tcagctcgac gttccagtac gccatgtcga
      421 ggaagttctt ccacgtcggg tggatccggt gcggcgacat gaggccgagt ggcgtcgctt
      481 tcggtttggc ggccggccgg cgcagcttca tgcccgcctc gtccggaagg cgattgccct
      541 ttttcacgtt gcagggcagg caggcgcaca cgatgttctc ccagttcgac gggccgccgc
      601 gcgacagcgg gacgatgtgg tcgagcgaga ggtccgacgt cgagaagcgg cggccgcagt
      661 actggcagcg gttcccgtcg cgcatgtaga tgttgtggcg cgagaacttc acctcgtgcc
      721 gcggcgagcg gtcgcacgag agcagctgga cgacgcgtgg caccttgagg acgaggctgg
      781 gagtcgtgac gacgtcgtcg tccgcctgga cgggaatgtc ctgccactcg tcccacgcgt
      841 aggtcgtgta gtccgggagg accgcccgca cgtgcccctt gtagaagagc gtgaaggtcc
      901 gcttcacgtc cgtcagggtc agcgccgtga accccttgtt caggaccagc accggcgagt
      961 cgagcatggc caagcatagc ccaacggctg ttaacgtagc gccccggatg aggatcctct
     1021 tcgtgcgcct ttcgtcgttc ggcgacgtgc tcttcgcgct gcccgcggcg aaggcgctgc
     1081 gagcctctct cgcggacgcg cacctgacgt gggcgatcga accgccgctc gcgccgctcg
     1141 tcgagggcgc cgcgtacgtg gacgacgtga tcggcgtcga cacgcgcggc tggcggcgcc
     1201 gaggattcgg aaaagagacg agagaagaga tttcggaatt tcttagaagg gtaagggggg
     1261 cggacttcga tctcgtcgtg gatgctcagg ggttgttcaa gtcggcgctc gtgacggcgg
     1321 cggcgcgctc cgggcgcaaa gtcggcttcg gtttcagggt tgcgacggaa tggacgaact
     1381 gcctcgtgac cgacgagcgc gtggacgtgg accgcgcggc gcgcccccac gtcctcgacc
     1441 agatgctcgc actcgcggaa cacgtcacgg ggaaaagcgg cttcgagagg acgccggacg
     1501 tgacgcacct cgtggagcgt ccggatcccg tggtcgattc atggctggag caaacgggcg
     1561 gccggccgtt cgcggtgttg cagcctttct cttcgaaggt gaacaaggag tggggcgcgg
     1621 acgacgtcgt cgcgttcgcc gcatggctcg tgtccgaggg aatccagcct gtgctccgct
     1681 gggggccggg agaagagagc cgagcggctg agctgattga cgcttctaag aaatcttcgc
     1741 ttctcccctc ttctctttct ctccctctcc tcctcgcgcc cccgacgaac cccgcgtcca
     1801 gcgcgcgcct cgcggcgcgc gccgcgctgt tcgtgggcgc ggacacgggg ccgacgcatc
     1861 tcgccgcggc tgccgggacg ccgacgctcg ccctcttcgg accgacgccg cccgagcgtt
     1921 tcgggcccgt ggggccccgc gtcgcggtcc tccgggaatc ttccgcggcc tacaatcgtt
     1981 cgggtggacg atggccagtc gagggcgtcc gtgaagccgc gagcagactc ctcgcgtagg
     2041 cgcggccggg cggaactcgt cctcgcggcc ggcgctctcg ccgcggcggc tttcctcgtt
     2101 ccggcccggc gctccggggc cgaccgcccc ccggtcgtgg tggcggcgcg cgagaccctc
     2161 ccgaaggacc gtctcttcgc cgtgccgttc ggcgagggcg agacgctcgt ctacacgatc
     2221 gcctggctga agatcgaggg cggcgggatg acgctcagga ccgcgcgcga gacgacctcc
     2281 gacggcgtcc cgatgcaccg catcacgctc acggccacct cgaacgacta cgtctcgaag
     2341 ttctaccccg ttcgcgacct ctacgaaacg tgggtggacg cgcgcgattt ccagcccctc
     2401 cgcttcgaga agcacgcgcg cgagggccgg tacgagtccg acgaggtcga ggagttcgac
     2461 ctcgccaaga agatcgggag ctggcgcgag gaccggacgc cgctgccgga ccggatccag
     2521 gacatcatct cgtcgttcta tttcctgagg gcccagcccc tcgccgtcgg gtcggacgtg
     2581 cgcgtcgaca tgttctcccg cgggaagatc tacaagctca aggccgccgt tctcgagaag
     2641 gaacgagtcg agacggaggc gggcgtcttc gacgcgttca aggtccagcc gcagctccga
     2701 gagaacgaga ccgccgagga ccgaaaccgc ggcaagctct tcctctggtt ctcggacgac
     2761 gaccgccgcc tcccggtcat ggcgaagacg gtcatgccca tcgggtcggt cacggcgcgc
     2821 ctcacgaagg tcgtcgccgg aacccgctag aatcccccgc atggccgtga agctgggcga
     2881 tctcctcgtc aaggcgaagc tcgtcacgcc cgagcagatc caggaagcgc tcgtcgaggt
     2941 gcaggcgtcg ggcatgaagc tcggcgaggc cctcgtcaag ctcggctacg tctcggaaga
     3001 cgacatcacg gagaccctct cggcgcagtt cggcgtcccg tcgatcaacc tctcgcactt
     3061 cgagatcgac ccgaacgtcc tcaagctcgt ggcccccgag gtcgcgcgca agtacaacat
     3121 cctcccagtc aacaagacgg gcgcgacgat cacgatcgcg atggcggacc cgacgaacgt
     3181 gttcgccatg gacgacatca agttcatgac gggctacaac gtcgagcccg tcgtcgcctc
     3241 cgagatcgca ctccagcagg cgatcgaccg ccactacggc gcgacgcacg ccctcgagct
     3301 gaagaaggtc atggaggaga tgtcggtcgt cgacacggcc gacacctcgc tcgaggtcgt
     3361 cgaggagtcc gagcaggaga cgatcgacct cgagaagctc gtcgacgagg gcgaggaggc
     3421 ccccgtcatc cggctcgtga acatcatcct gacggacgcg atcaagcgca gcgcgtccga
     3481 catccacatc gagccctacg agcgcgagta ccgcgtgcgg taccgcatcg acggcatcct
     3541 ctacgaggtc atgaacccgc ctccgaagct caaggaggcg atcgcgtcgc gcatcaagat
     3601 cctcgcgaag ctcgacatcg ccgagaagcg cctcccgcag gacggtcgca tcaagatcaa
     3661 gatgaagctc gcgggcaagg tcaaggagct ggactaccgc gtctccgtgc tcccgacgct
     3721 cttcggcgag aagatcgtct gccgcctcct cgacaaggac aacctcatgc tcgacatgac
     3781 gaagctgggg ttcgagaagg actccctgct tcgcttcgag aaggcgatcc tgcgcccgtg
     3841 ggggatggtc ctcgtgaccg ggccgacggg ctcgggcaag acgaacacgc tctactcggc
     3901 gctctcgcgc atcaacacgc ccgagacgaa catcatgacg gccgaggacc cggtcgagtt
     3961 caacctcccg ggcatcaacc aggtccagat gaaggacgcg atcggcctga acttcgcggc
     4021 cgcgctgcgc tccttcctcc ggcaggaccc gaacatcatc ctcgtcggcg agatccgcga
     4081 cttcgagacg gccgagatcg cggtcaaggc ctcgctcacg ggccacctcg tcctctcgac
     4141 gctccacacg aacgacgcgc cctccacgat caaccgcctc atgaacatgg gcatcgagcc
     4201 ctatctcgtc gcgacgtccg tcgtcctcat cgcggcgcag cgcctcatcc ggcgcgtgtg
     4261 cgcgagctgc aaggcgccga ccgaggtcgc gccgcaggtc ctcgtctcgc tcgggttcac
     4321 gcccgaggac gcgaagaccg tgcaggtcat gaagggcaag ggctgcgaga agtgcaacaa
     4381 cacgggctac aagggccgcg tcgcgctcgt cgaggtcctc gagatcaccg aggaggtccg
     4441 cgagctcatc ctctcgggcg cgtcctcgat cgagatcaag aggaagggcc tcgaggaggg
     4501 catgatctcc ctccgccgct cgggcctgca gaagatcaag gacggcctca cgtcgatcga
     4561 ggaagtcgtg cgggagacgg tgctctgacg gtgggtgcgc ggcccgaaga agtctatttc
     4621 ttcgggacct gcgtcgtgga cctgttcttc cccgaggcgg gcctcgccgg aatcgagctc
     4681 ctcgcgcgcg aaggcgtgcg cgtcgttttc ccccgggcgc agacgtgctg cgcccagccc
     4741 gcgttcaact gcggccacca cgaagaggcg cgggccgtgg ctcgcgcgca gatggcgctg
     4801 ttcccgaagg cgatccccgt cgtcgttccc tccggatcgt gcgccggcat gatgaagcac
     4861 cattacccgg agctcttctc gggaacaccg gatcacgagg ccgcggtcgc attcgccgac
     4921 cgcgtgcacg agttgacgca attcctcgcc ggggatctcg gggtctccct cacggaccgc
     4981 ggcccaccgg tcgcggtgac ctggcacgcc tcctgtcacg gcctgcgcga gctgggcctc
     5041 gacgcggagc cgaaggctct tctggcccag ctcgcgaacg tcacggtcgt tccgctcgcg
     5101 cgggagcgcg aatgctgcgg gttcggaggc acgttctcgg tccgctaccc ggacgtttcg
     5161 tctgccctcg tggcggacaa ggcggtcgac gtcgaggcga ccggcgcgcc ggtcgtcctc
     5221 tccgccgacg ccgcctgcct cctcaacatc ggcggagcgc tcgacgcgcg agggacggcc
     5281 gtcgcggctc gtcacctcgc cgagttcctc tgggagcgca ctcgtgcgcg ctgacgacga
     5341 gacccctgca tcaccggact tccacgccgc gtcgtcgcgg gctctcgcgg acgaaggtct
     5401 ccgcgcgaac tttcgccgcg ccatggacgg cctcgtcggc aagcgagccg cccagttccc
     5461 ggactcggcg gagtggcgaa ccttgcgcgc ccgcggcgcg gagatccgcg cccgtgcact
     5521 cgcccgcctg cccgagctcc tcgaaaggct cgagcagaag tgcgccaaga acggcatctt
     5581 cgtccactgg gcctcgacga cggaggaagc caacgggatc gtcctcgaga tcctgcgccg
     5641 caacggcgcc acgcggctcg tgaaggggaa atcgatggtc tccgaggaga tgcatctcaa
     5701 tgagttcctc gaggagaacg gcgtcgagtc cctcgagacg gacctcggcg agtacgtcat
     5761 ccagctcgac ggcgagacgc cctcccacat catcatgccg gcgatccaca agaaccggca
     5821 gcagatcgcc cgcctgttcg gacagaagat ccgggacgcg gtgtacaccg aggacgtgga
     5881 cgccctgacg gccatcgcgc gtcgggtgct gaggcagaag tttctcgagg ccgaagccgg
     5941 tctctcgggc gtcaacttcg cggtcgccga gacggggacg ctctgcctcg tcgagaacga
     6001 ggggaacggg cggatgtcga cgcacgtccc gcccgttcac atcgccgtga tgggactcga
     6061 gaaggtggtc gagaagctcg aggacgtacc tcccctgctc acgctgctca cgcggtccgc
     6121 cacggggcag ccgatcacga cgtacgtcaa catgatctcg gggccgcggg gtccactcga
     6181 gaaggacggt ccgcgggaag tccacctcgt gctcctcgac gccggccgct cgcggatctt
     6241 cgcggatccg gagctgcggg ccacgcttcg ctgcatccga tgcggcgcgt gcatgaacca
     6301 ctgccccgtc tactcgcgcg tcggcggcca cgcgtacgag gccgtgtatc cagggccgat
     6361 cggacaggtc ctcacgccgc agtccgaagg actcgggcgc cgccacgacc ttcccgacgc
     6421 ctcgagcctt tgtcacgcgt gcacggaggt ctgtcccgtc gagattccga tctcgaagct
     6481 gctcgttcgg ctgagggcgg aggccgcgca gccgcccgcc ggatcgaacg tcctcggcgc
     6541 gggaggcgga cgcagccgtc tcgaggacct cgcgtggggc gcgtggaagg cggcccacgt
     6601 ctcgcccctc ctccatcgcg cggcgaccag ggtgttccgg ctcgtccacc cgctgatccc
     6661 ctcgtggctc ggacccatga ggggctggac gaagtcccgg acgaagccga gacccgcggc
     6721 gaggacgctg cacgagctcg tgagagagaa ggggcttccg gatgcctgac ggggcgcgaa
     6781 ccgccatcct cggccggctc cgcgccgtcg caggtccgac cgcatccggc cgaaaagacg
     6841 cggtccccga tctccttcca gagaaatact cgtcttcgtc ctcgtccgac cgcgtcctgc
     6901 ggttccgccg atgcatggaa gccgcccgaa ccgagtttct ggaggcgacc gaggccgcct
     6961 ggccggagaa gttgaacggg tttctcctcg ccgagggcct gcgccgcgtc gccttccctc
     7021 ccgcgacgga cgcgggccgc cgcctcaccg cggcgtgtcc cgcggggggc gacgtcacgc
     7081 tcgtgcccta cgaccgcccg gtcgaggaga tgaaggacgc cctcttctcg gaaatcgacg
     7141 ccgggttcac gacggcacgg ggcgccatcg ccgagacggg cagcctcgtg ctctggccct
     7201 cgccggagga gccgcgtctt ctgtccctcg tcccgccgat ccacatcgcg ctcctcgacc
     7261 gccgcacgat ccgggacacg ttcgaggacg ttctgcgcga ggagcgttgg gcggaaggga
     7321 tcccacccaa tgcgctcctg atctcgggcc cgagcaagac ggccgacatc gagcagaccc
     7381 ttgcctacgg cgtccacggc ccgaagcgcc tcgtcgtcgt cctcgtgtaa cggcgcgccg
     7441 ctcgcggtac agatctcatg ctgctagaat ctgcatcgag aggcagatcg tgcgcacgac
     7501 gctgaacatc gacgacgcgg cgcttcaggc ggcgctcgcg gccgaccccg ggaagacgaa
     7561 gacggccgtc atcaacgccg cgctgcgcga ctacgcgcgg cgtcgcgcgc tccgcgggct
     7621 gagaaggctc gcgggcaagg ccggctggtc gggagatctc gacgcgctcc gcaagcggcg
     7681 gccccgggcc tcgtgagggt cctcgtcgac acgtcggcgt gggccgagtt cctgaacggg
     7741 acggcctcgc ccgaggcgag ggccgtcgac cggctcctcg cgggtgagga cgacgtctgc
     7801 acctgcgggc tcgtggtcgc cgaggtcttt cagggaatcc ggcgcgaccg cgaagaggtg
     7861 gaggcgttct tccggaagat gacgttcctc gagccctccg ggatcgacgt atacctgaag
     7921 gccgccgacg tccaccggac gctgcgccgc cgcggcgtca ccatccgctc aacgatcgac
     7981 tgcttcctcg ccgtcctcgc cgcggaaaac ggctgcgcgc tgctcttccg ggaccgcgac
     8041 ctcgaccgga tcgtcgcgag cggcctcctc gacctccccg cccgagcctg aagcgcctca
     8101 ggccgcctcg cggtctccgt cggcgcggcg gacgcgcgcg aagagcgccg cgagctgcgc
     8161 gtcgtccagc gtctcctcgt cgagcagccg ccgagccccc tcctcgagga gcggacggtt
     8221 cgtcctgagg atcccggacg cccgttcgta cgccgcggcc aggagctccc gcaccgcacg
     8281 gtcgatctcc cggttcgtct cgtcgccgac gagccggcgc ccgtgggcga gcggtccgcc
     8341 cggggccagg aaggagcctc cgtcgtcgtc gtaggcgacg tgcccgagtt ccggcaccat
     8401 gccgtagcgc gtgaccatcg cgcgcgcgat cgaggtcgcc ttggcgaggt cgtccgccgc
     8461 tccggtcgag aggtggtcga agacgatctt ctcggccgcc cgccctccga gcagcaccgc
     8521 catcttggcc tcgagctccg cgcgcgtcat caggtagcgg tcgtcggtcg gccgctggat
     8581 cgtgtagccg agggctccga tcccgcgggg aatgatcgag accttgtgca cgacgtccat
     8641 ccccgggagg gcggcggcga cgagcgcgtg gcccatctcg tgatgggcga cgatcgtccg
     8701 ctccgcgtcg ttgagcagcc tgttcttgcg ctcgaggccc gcgacgatcc gctcgatggc
     8761 cgccgtgaag tcctcgcggc tcacctccgt ggcgttccgg cgcgtcgcgg ccagcgccgc
     8821 ttcgttgacg aggttggcga gatcggcgcc cgtgaagccc ggcgtgagcg ccgcgacctc
     8881 ctcgaggttc aggtccgcgc ggaccttcac ccgcgccgcg tgaaccttca ggatgtccgc
     8941 tcgtccgcgc ttgtccgggc ggtcgacgag gacctgccgg tcgaagcggc ccgcccgcag
     9001 gagggcggga tcgaggatct cgggccggtt cgtggccgcg aggagcacga tgccgctgcg
     9061 cgggtcgaat ccgtccagct cgacgaggag ctggttgagc gtctgctcct tctcgtcgtg
     9121 cccgccgaac cccggctgcg agtttctcgc cttgcccagc gcgtcgagct cgtcgatgaa
     9181 gatgatgcaa ggggccttca gccgcgcctg ctcgaagagg tcgcgcacgc gcgcggcgcc
     9241 gacgccgacg aacatctcga cgaactcgga gccgctgatc gagaagaagg ggacgccggc
     9301 ctcgccggcc acggcccttg cgagcagcgt cttgccggtc cccggcggcc cgacgagcag
     9361 gattcccttc ggggcccgcg cgccgaggcg cccgtacgag cccgggtcct tcaggaacga
     9421 gacggtctcc gccagctcgg ccttcgcctc gtcgacgccg gcgacgtcct ggaacgtcac
     9481 cttcgtgtcc gtctccacgt agaccttcgc cttgctcttg ccgatcgaga ggaggccgcc
     9541 cgatccgccg cccatccggc ccgcgaggaa ccggcccagg aggatccagg cgccgaagaa
     9601 gatcaggacc gggacgaccc acgacagcag cgtcgggaga agcgtctcct cggcgcgcgc
     9661 ggcatacttg atcccgtgcg cgtcgagctc gcgcgcgagc ccgggctcca cgcgcgtcgc
     9721 gacgaaccgg ctgcggccgt tctccggcgc cgtcagctcg ccttcgatcc ggtccgcggt
     9781 gacgacgacc tccttgacct tcttctccct gaggagccgc tggaactcgc tgtaggggat
     9841 cgccgcgacc tgccggctcg cggtccacgc gtcgtggagc aggaagacgc cgacgacggc
     9901 gaggagcagg taggtcacgt tgaagcgcgt cgacttgagc atgtccgtct ctcctcagct
     9961 cccgctcgga accgcggcgg gcttcggcgg ccggacgagc gagaggacga tcgagatccc
    10021 gagaatggcg gcgatgacga cgagcgacgc cgcgatcgcg aagaatccta tgcccggtcc
    10081 taccactccg gcaggaggcg cggcaagagc ggagaacccg cgtccagggg cgcacgcccc
    10141 gccatggcga gaaggcgctc gacgcgctcc tcggtccggg ggtgggtgga gaaccacctc
    10201 gagagcgact cgaccgcccc gaacgggttc acgatgaaca ggctcgccgt cgccggcgcc
    10261 gcgtccgccg ggacgaggtg cgacgcctgc tcgagcttca cgagcgcgcg ggcgagggcc
    10321 tccggttcgc cggagatccg ggcgcccgtc tcgtcggcca tgaactcgcg cgcgcgggaa
    10381 atgccgaact ggatgagcgt cgccgcgagc ggcgccacga ccgcggcgag aaggcccccc
    10441 gccgcgccgc cctcggactc ctcctcctcg gacccgccga agaacgcgcc gaagctcacc
    10501 gcctgcgcga cgtaggtgac gagcgaggcc gccgcggccg cgaggctcga gacgaggatg
    10561 tcccggttct tgatgtgcgc gagctcgtgc gcgatcaccc cgcggagctc gcggcggtcg
    10621 aggaggcgca cgaggccctc cgtcaccgcg acgaccccgc gctcggggtt ccgccccgtc
    10681 gcgaacgcgt tcggctgctc ctcgggaatc aggaagacgc gcggccgcgg gatccccgcc
    10741 ctcgcggaga gctcctcgac gatcgcgtgc agctccggcg cctcgtccgc tccggcctct
    10801 cgcgcgtggt gcatccggag gacgatgcgg tccgagaaga agtacgcgcc gaggttcacc
    10861 gcgagcgcga gcgcgccgaa cagggccagt gaccccggcg cgacgagcgc gccgaggccg
    10921 acgatgatcg cggacagcga gccgaggagg acgacggtct tcagctggtt tttcacgaca
    10981 gttctccttg gggagttccc atgatctcga ggcccggatg cgcgcgccgc cgccgcggga
    11041 gccggaacgg cgccgggccg ggtccgcggg ag
//
LOCUS       DAOMFY010000117        11066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1836_length_11066_cov_6.747525, whole genome shotgun sequence.
ACCESSION   DAOMFY010000117 DAOMFY010000000
VERSION     DAOMFY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11066
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1836_length_11066_cov_6.747525"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PLB01_10760"
     CDS             complement(<1..1043)
                     /locus_tag="PLB01_10760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ77827.1"
                     /translation="MTLSPGARLGPYEILAPLGAGGMGEVYRAKDTRLARDVAVKVLP
                     EEFFEGEERRARFEREARLLAALNHPGIAAIYSFEEVPSSSSSSSRHLLVMELVEGEG
                     LDQKIAAGPLPLEESLSFARQIAEALEAAHEKGIIHRDLKPANVRVTPDGRVKLLDFG
                     LAKMFEGDGGSGSSAGVTHSPTLTARATAAGVILGTAAYMSPEQARGKPVDKRTDVWA
                     FGCVLFEMLTGKRAFEGETVTDVLAAVLMKEPDWAALPERTPGKIRDLLRKCLRREAK
                     QRLHDIADARLDLEELAATASSAGRLPFEENLNSAAPGPSAGRTAPATSASGSRTSLY
                     LSWALAAAFIVEAA"
     gene            complement(1090..1989)
                     /locus_tag="PLB01_10765"
     CDS             complement(1090..1989)
                     /locus_tag="PLB01_10765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQQ77828.1"
                     /translation="MKTARLALLVASAFLTSPLVAQAPDPHAEHAKEAAARPSPAAAP
                     APRNEAIPPDADQAKDALAKSPRHGEWVDVALPGGGKLLTWVVYPEKKEKAGIVIVIH
                     EIFGLTDWVRGVADQLAKDGFVALAPDLLSGMGPNGGGTESLGADATKAIRNLTPAEA
                     VARLDAVRAYALKLPAANGKVGAVGFCWGGSASFGYAVAQPKLDAAAVYYGSSPEDSE
                     AYSKIGAPVVGFYGGDDARVNATIPRAEEAMKKLGKRYTANVYEGAGHGFLRQQTGRD
                     GANARASAKAWAATLGFFRENLK"
     gene            complement(1986..4499)
                     /locus_tag="PLB01_10770"
     CDS             complement(1986..4499)
                     /locus_tag="PLB01_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77829.1"
                     /translation="MTLSKGTVLGAYEIVAPLGAGGMGEVYRARDPRLNRDVAVKVLP
                     EDFLEGEEKRARFEREARLLAALNHPNVAAIYSFEEIPSSSSSSSPSLQHILVMELIE
                     GETLRHAFPPEPAATKRILEVGAQIADGLAKAHASGIVHRDLKPENVMVTRDGWVKIL
                     DFGLAKLTEARSQELSSAATATGTMPGMVMGTVGYMSPEQASGKPVDFRSDQFSLGTI
                     LYELATGRRAFQRDTSAETIVAVMRDEPEPVGKLNPRVPAPLRWIIDRCLAKDPEQRY
                     ASTRDLARDLASLRDRLGELSGESTAGVAEPVSAHPTFQRLSFRRGTILSARFAPDGR
                     TVVYGAAWEGEPCRLFSTRPESPESSPLMLPDAEILAISRTGQLAISLERHWAGRFIW
                     SGTLAQVPLAGGAPRELLENVHWADWGPDGAHLAVVRSVAGKTRLEYPIGKVLYETGG
                     WISHPRVSPKGDLIAFLDHPTLGDDEGSVATVDRTGKVAVLSTDWITTYGLAWSGDGQ
                     DVWFTGTRVGVARSVWSVSLAGKERLLVRTPGELTIQDASSDGRILMTSDNGKVGVIG
                     RPPGSDRDRDLSLLDWSLVRDISKDGRQILFDESGEGGGARHAVYVRRTDGSPAVHLG
                     EGWAGNFSPDERWVASQSMSPEGRRISLLPTKAGERRELPKDDLRVHRAWWLPDGKRI
                     LAAANAPREGLRLFVHGLEGEPPRPITPEGIGMGFFPVSPDARVVVAQGPDGLFYGFP
                     IEGGDPEYVPGLAAEDRPIRFSPDGKSLFAYRRGELPAHVIRLDLATGEKHASQELMP
                     PDAAGVVEIVSVVLTPDVASYAYSYHRILSDLFLVEGLR"
     gene            complement(4496..5791)
                     /locus_tag="PLB01_10775"
     CDS             complement(4496..5791)
                     /locus_tag="PLB01_10775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQQ77830.1"
                     /translation="MSRRLAAPALALVLAGAALSRPVLAQDPRPRADAAGIAPDRLAR
                     VDAAFREAVASGKIAGAVVYVARGGRAVVFEAFGALDVEKKVPMPTDAIFRIASMSKA
                     VTTVAALLLLEEGRITLDAPVSRWIPSFAKTTVYVAPAKDAPAGTPGQVVPAKRPITV
                     RDLMTHTAGISYGTGALEDRYKAANAWWWYLSDKDETLASFVDRLATLPFEAQPGERF
                     VYGFSTDVLGRVVEAASGQSLDAFFETRIFRPLKMPDTSFFLPPEKRDRLATVYGLTE
                     SGKIERAPEKGHTGQGAYVEGPRRCFSGGAGLLSTAADFGRFLQMLLNGGVLDGVRLL
                     SPKTVALATSNHVGALYEEGRLGFGLGFEVTEKLGPAGRLDSAGAYGWGSAYYPRFFV
                     DPQEKLVAFFLVQLIPARDYDLHKRFRTLVYQSIVGGTN"
     gene            complement(5788..8529)
                     /locus_tag="PLB01_10780"
     CDS             complement(5788..8529)
                     /locus_tag="PLB01_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77831.1"
                     /translation="MTISSGTRLGPYEILAPLGAGGMGEVYRARDTRVDRAVALKVLP
                     EEFFESEERRGRFEREARMLASLNHPGIAVLYSFEEIPSFSSSPSSSPTRHLLVMELV
                     EGDDLAVRVARGPLPLEESLSFARQIAEALEAAHEKGIVHRDLKPANVKITPDGRVKL
                     LDFGLAKIFESEATLSNPSISYSPTLTARGTAAGVILGTAAYMSPEQARGKPVDKRTD
                     VWAFGCVLYEMLAGKRAFEGETVSDTLAAILRGEPDWAALPEQTPAAVRRILRRCLQR
                     DARARLHDIADARLELEEMSAPGASGTFTFEEKQAVPSHTGAGAERTSRERGSRKSLY
                     LSWAIAAAFAAAAGALALRARVPRAPAPVVQFEVRVPEGATFGNSMALSPDGRRLAFV
                     ATDEKDVDRLYVRSFDTIGAQVLAGTEGARFPFWSPDGASIAYFTGDRLARVATAGGP
                     PLTICPVTDGRGGSWSPRGIIVFAPGIRVPLHRVSASGGIPERVTTLGPKDYTHRWPK
                     FLPDGARFVFLSLSDDPARSGTRVASLDSSDDTFVVSTRGKAEYEAGRLLYVRENALY
                     SQKFDGRKLSGEPGLVVDGIVVEGDSGWTGLAAFSAADGTLAYRRRANPRQKLTWFDR
                     SGKPLGTVGEAGVMSEPFWLVGGQRLGITVTDRRTDLPDLWQVDLARGAWTRLTFGPK
                     SNNTGIASPDGKYLYFSSNREERMGLYRRPLDGTGGDEPLLATTLDNYGDFISPDGAW
                     LVYESQGATGRAQLWRLPLGGDRKPSPLLSMPQAATAHASGSPDGRFFAYSSDETGRS
                     EVYVQKFPPSGGKWQISTDGGDQALWRADGKELYFLSNDRKLMAVDVTLGGDVQVGVP
                     KVLFPVRVPANGISDNRSQYIPAPDGTRFLVLATADDRQDQPAVVVLNWTSIGGAPR"
     gene            complement(8571..9740)
                     /locus_tag="PLB01_10785"
     CDS             complement(8571..9740)
                     /locus_tag="PLB01_10785"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HQQ77832.1"
                     /translation="MQRRAIVVVCDGLGVGEAPDAAAFGDAGARTLQHILRDGRPVIP
                     HLTKLGLLHTMYGEAPAGLPAPSGAFGRLAEASAGKDSTTGHWELMGLTVPVAFPLYP
                     DGFPPEVVEPFEKAVGKKVLLNRPASGTEVIKLYGEEHMKTGRPILYTSGDSVFQIAA
                     HEDVVPIETLYRWCEIARDMLDGKHRVGRVIARPFVGAPGSFTRTHRRHDYTVPPDGP
                     TLLDELTAQGKRVYGIGKIEDLFSGRGVPKAVHTESNRDGLEKTVAALSGRDDDFVFA
                     NLNDFDTKFGHRNDPQGYARALEELDSFVPALLAGLREGDVLMVTADHGGDPSDVSTD
                     HTREFVPMVAAGPRVRAGADVGTRATFADLGATVAEHLGVPPLAGASFLSAILRG"
     gene            complement(9741..10451)
                     /locus_tag="PLB01_10790"
     CDS             complement(9741..10451)
                     /locus_tag="PLB01_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77833.1"
                     /translation="MARLTKDQLRTDQLEHALTDARDFVSSHRSQTARWIAIGAAAAV
                     VVIAIWGGLQWKSNRAASRLSAALAIFDAPLASDPSPAPGAKVYKDAAERTAAARSAF
                     QELVKDSPSSASGRAAAVLLLGIDGGSAATGTNLDAARAFAKSEKGTVAAGVAAVAAL
                     DAESAAGRSKEALEVAKRYLDSADAPLPKDVLVYSVAKLYEKTGQNVEAKSFYQRLVT
                     DFPDSPLRTEAQQKLAGL"
     gene            complement(10472..10786)
                     /locus_tag="PLB01_10795"
     CDS             complement(10472..10786)
                     /locus_tag="PLB01_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HQQ77834.1"
                     /translation="MEESNRIRVTDRALAALRAEGWEPGRTCLVVRHVLGCGGSGYRI
                     TFSERPLEDGRRVDAGGGLVVWLDPYSYSRLDGASIDYDPEKETEGFLLDHPDAAFAA
                     FC"
     gene            complement(10795..>11066)
                     /locus_tag="PLB01_10800"
     CDS             complement(10795..>11066)
                     /locus_tag="PLB01_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77835.1"
                     /translation="SPAAKPAPAAAPKKKAAPKKKKAAPKKKAAKKAKAAPKKKAARK
                     AAKKKAAPKKKAARKPARKAVKKTKRAKRAAPKRKAKKAAKRKKR"
BASE COUNT         1637 a         3833 c         3860 g         1736 t
ORIGIN      
        1 gccgcctcca cgatgaacgc ggccgcgagc gcccaggaaa gatagagcga agttctgctc
       61 ccgctcgcag acgtcgccgg agcggtccga cctgcactgg ggcctggcgc cgcggaattg
      121 agattctctt cgaagggtaa tcggccggcg gagctggccg tggcggcgag ctcttcgagg
      181 tccaacctcg cgtccgcgat gtcgtggagg cgttgcttcg cctcccgcct cagacacttt
      241 ctaagcagat ctcttatctt ccccggcgtg cgctccggca gcgccgccca gtccggctcc
      301 ttcatgagga ccgccgcgag gacgtcggtg acggtctctc cctcgaaggc cctcttcccc
      361 gtcagcattt cgaacagcac gcacccgaac gcccagacgt ccgtccgctt gtcgaccggc
      421 ttcccccgcg cctgctcggg cgacatgtac gcggccgtgc cgaggatcac gcccgccgcc
      481 gtcgcgcgcg cggtcagcgt cggcgagtgc gtgacgcccg ccgaggagcc ggagccaccg
      541 tccccctcga acatcttcgc aaggccgaag tcgaggagtt tgacgcggcc atcgggagtg
      601 acccggacgt tcgcgggctt gaggtcgcga tgaatgatcc ccttctcgtg cgcggcctcg
      661 agggcctcgg cgatctggcg agcgaaggaa agagattctt cgagtggaag agggccggcg
      721 gcgatcttct ggtcgagccc ttcgccttcg accagctcca tgacgagcag gtggcgggac
      781 gaagaagaag aggaagaggg gacttcttcg aatgagtaga tggcggcaat tccgggatgg
      841 ttgagcgctg cgagcagccg cgcctccctc tcgaatcgcg ctctcctctc ttcaccttca
      901 aagaactctt ccggcaatac cttcaccgcg acgtctctcg cgagcctcgt atcttttgcc
      961 cggtagacct ctcccatccc ccccgctccg agcggcgcga ggatctcgta aggccccagt
     1021 cgggctcccg gtgaaagcgt cacggtgtga gcggattcta gctgcgacgg agacgccggc
     1081 gctcgggccc tatttcaggt tctcgcggaa gaacccgagc gtcgcggccc aggccttcgc
     1141 ggaggcgcgg gcgttcgcgc cgtcgcggcc cgtctgctga cggaggaagc cgtgtcccgc
     1201 cccctcgtac acgttcgccg tgtatcgctt gccgagcttc ttcatcgctt cctcggcgcg
     1261 agggatcgtg gcgttcacgc gcgcgtcgtc gccgccgtag aacccgacga cgggagcgcc
     1321 gatcttcgag taggcctccg agtcctcggg cgacgacccg tagtagacgg ccgctgcgtc
     1381 cagcttcggc tgcgcgactg cgtagccgaa gctcgccgaa ccgccccagc agaacccgac
     1441 cgccccgacc tttccgttcg cggcggggag cttgagcgcg tacgcgcgca cggcgtcgag
     1501 gcgcgccacg gcctccgcgg gcgtcaggtt gcggatggcc ttcgtcgcgt cggccccgag
     1561 cgattccgtg cctcccccgt tcggccccat tcccgagagg agatccggcg cgagggcgac
     1621 gaacccgtcc ttcgcgagct ggtccgcgac gccgcgcacc cagtccgtga ggccgaagat
     1681 ctcgtggatc acgatcacga tcccggcctt ctccttcttc tcggggtaga cgacccacgt
     1741 caggagcttt ccgccgcccg gaagcgcgac gtccacccat tcgccgtgcc gcggcgactt
     1801 cgcgagcgcg tccttggcct ggtccgcgtc cggcgggatc gcctcgttgc gcggcgcggg
     1861 cgcggcagcg ggcgacggcc tcgccgccgc ctccttcgcg tgctcggcgt gcgggtcggg
     1921 ggcctgggcg acgaggggcg aggtcaagaa tgccgaggcg acgaggagag caaggcgggc
     1981 ggttttcatc ggagtccctc cacgaggaag aggtcggaca ggatgcggtg gtagctgtag
     2041 gcgtaggacg ccacgtcggg cgtgagcacg accgacacga tctccacgac gcccgcggcg
     2101 tcgggcggca tgagctcctg agaggcgtgc ttctcgcccg tcgcaaggtc caatcggatc
     2161 acgtgcgcgg gaagctcgcc gcgccggtac gcgaagagcg acttgccgtc gggcgagaaa
     2221 cggatcgggc ggtcctcggc ggcaaggccc ggaacgtatt cgggatcccc gccttcgatc
     2281 gggaatccgt agaagagccc gtcggggccc tgcgccacga cgacccgcgc gtcgggcgac
     2341 acggggaaga accccatgcc gatgccctcc ggcgtaatcg gccgcggcgg ctcgccttcg
     2401 aggccatgca cgaacagacg gagcccctcg cgcggggcgt tcgctgcggc gaggatccgc
     2461 ttcccgtccg ggagccacca ggcgcgatgc acgcgcaggt cgtccttcgg gagttcgcgc
     2521 cgctcgcccg ccttcgtggg caggaggctg atccggcggc cttccggcga catgctctgc
     2581 gacgcgaccc atcgctcgtc cggtgagaag ttgcccgccc agccctcccc gagatggacg
     2641 gccggcgagc cgtcggtgcg gcgaacgtac acggcgtggc gcgcgccgcc gccctcgccg
     2701 ctttcgtcga acaggatctg cctgccgtcc ttcgagatgt cgcgcacgag ggaccagtcg
     2761 aggagggaga ggtcccggtc ccgatcggag ccgggggggc gcccgatgac gccgaccttg
     2821 ccgttgtcgc tcgtcatgag gatccggccg tcggaggacg cgtcctggat cgtcagctcg
     2881 cccggtgtcc gcacgagaag gcgctctttt cccgcgagcg agacggacca gacggagcgc
     2941 gcgacgccga cgcgcgtgcc cgtgaaccac acgtcctgcc cgtcgcccga ccacgcgagg
     3001 ccgtacgtcg tgatccagtc cgtggacagc acggcgacct tgccggtccg gtcgaccgtc
     3061 gccaccgacc cctcgtcgtc gccgagcgtc gggtggtcga ggaacgcgat gagatcgcct
     3121 ttcggcgaga cgcgcggatg gctgatccat ccgcccgtct cgtagagcac ctttccgatc
     3181 ggatactcga gtcgcgtctt ccccgcgacg gaccgcacga cggcgaggtg ggctccgtcc
     3241 ggcccccagt cggcccagtg cacgttttcg agaagctcgc gcggcgcgcc gcccgcgagc
     3301 ggcacctgcg cgagcgtgcc gctccagatg aagcgcccgg cccagtgtcg ctcgagggag
     3361 atcgcgagtt ggccggtccg cgagatcgcg aggatctcgg catcggggag catgagcgga
     3421 ctggactccg ggctctcggg ccgcgtcgaa aagagccggc acggctcgcc ttcccaggcg
     3481 gcgccgtaga cgacggtgcg gccgtccggc gcgaaccgtg ccgagaggat cgtgccgcgg
     3541 cggaagctga gccgctggaa cgtcggatgc gcggagaccg gctcggcaac gcccgccgtg
     3601 gattccccgg acagctcgcc gaggcggtcg cgcaggctcg cgaggtcgcg cgcgagatcg
     3661 cgggtcgacg cgtagcgctg ttcgggatcc ttcgcgaggc agcggtcgat gatccagcgg
     3721 agcggcgccg gcacgcgcgg gttgagcttt ccgaccggct cgggctcgtc gcgcatgacg
     3781 gcgacgatcg tctcggcgct cgtgtcgcgc tgaaaggcac ggcggccggt tgcgagctcg
     3841 tagaggatcg ttccgagcga gaactggtcg gaccggaagt cgaccggctt gccggacgcc
     3901 tgctcgggag acatgtaccc gacggtgccc atcaccatcc cgggcatcgt ccccgtggcc
     3961 gtggccgcgg acgacagctc ctgcgagcgc gcctcggtca gtttcgcgag gccgaagtcg
     4021 aggatcttca cccagccgtc gcgcgtcacc atgacgttct cgggtttcag gtcgcgatgg
     4081 acgatcccgg acgcgtgggc cttcgcgagg ccgtcggcga tctgcgcgcc cacctcgagg
     4141 atcctcttcg tcgctgcggg ctccggcggg aacgcgtgcc gcagcgtttc gccctcgatc
     4201 agctccatga cgaggatgtg ctgcagggag ggagaagaag aggaagacga agaggggatt
     4261 tcttcgaagg aatagatcgc cgcgacgttc gggtggttca gcgccgcgag caaccgtgct
     4321 tccctttcga agcgggctct cttttcttca ccttctaaga aatcttccgg caataccttg
     4381 accgcgacgt ctcggttgag cctcgggtcc cgcgccctgt agacctctcc catcccgccc
     4441 gctccgagcg gcgcgacgat ctcgtacgcg ccgaggacgg ttcccttcga aagagtcaat
     4501 tcgtccctcc gacgatggac tggtagacga gagtgcggaa cctcttgtgg aggtcgtagt
     4561 cgcgcgcagg aatgagctgc acgagaaaga acgcgacgag cttctcctgc gggtcgacga
     4621 agaagcgcgg gtagtacgcg ctcccccagc cgtacgcgcc cgcggagtcg aggcggcccg
     4681 cggggccgag cttctccgtc acctcgaacc cgaggccgaa accgagccgg ccctcctcgt
     4741 agagcgcccc gacgtggttc gacgtcgcga gcgcgactgt tttcggcgaa agcaggcgga
     4801 cgccgtcgag cacccctccg ttcaagagca tctggaggaa gcgcccgaag tcggcggcgg
     4861 tcgagagcag tccggcgccg cccgagaagc accggcgagg cccttccaca tacgccccct
     4921 gccctgtgtg tcccttctcg ggagcccgct cgatcttccc ggactccgtg aggccgtaca
     4981 ccgtcgcgag gcggtcgcgc ttttcgggcg gaaggaagaa cgacgtgtcc ggcatcttca
     5041 gcggccggaa gatgcgcgtc tcgaagaacg cgtcgagact ctgccccgag gcggcctcga
     5101 cgacgcgacc gaggacgtcc gtcgagaagc cgtacacgaa ccgctccccg ggctgggcct
     5161 cgaacggcag ggtcgcgaga cgatcgacga acgacgccag cgtctcgtcc ttgtccgaga
     5221 ggtaccacca ccacgcgttc gcggccttgt accggtcctc cagcgcgccc gtcccgtacg
     5281 agattcccgc cgtgtgcgtc atgaggtccc ggaccgtgat cggacgcttc gccggcacga
     5341 cctgcccggg cgtcccggcg ggcgcgtcct tcgcgggcgc gacgtagacc gtcgtcttcg
     5401 cgaaggacgg gatccagcgg gaaaccggcg cgtcgagcgt gatccggccc tcctcgagga
     5461 gcaggagcgc cgcgaccgtc gtgacggcct tggacatgga cgcgatgcgg aagatcgcgt
     5521 ccgtcggcat cgggaccttc ttctcgacgt cgagagcgcc gaacgcctcg aagaccacgg
     5581 cacggccgcc ccgcgcgacg tagacgaccg cgccggcgat cttgccgctg gcgaccgcct
     5641 cgcggaaggc ggcgtccacg cgcgcgaggc ggtcgggcgc gattcccgca gcatcggctc
     5701 ggggccgcgg gtcctgcgcg aggacggggc gcgagagcgc ggcccccgcg aggacgagtg
     5761 cgagggccgg agccgcgaga cgccggctca tcggggagcc cctccaatgg acgtccagtt
     5821 caggacgacg acggcgggct gatcctgccg gtcgtccgcg gtcgcgagca cgaggaaccg
     5881 ggttccgtcg ggcgccggga tgtactggga gcggttgtcc gagatcccgt tcgcgggaac
     5941 gcgcacggga aagagcacct tcggcacgcc cacctgtacg tcgccgccca aggtgacgtc
     6001 gacggccatg agcttgcggt cgttcgagag gaaatagagc tcctttccgt ccgcgcgcca
     6061 gagagcctga tcgccgccgt ccgtcgagat ctgccatttc ccgcccgacg gcggaaactt
     6121 ctgcacgtag acctccgaac ggcccgtttc gtccgaggag tacgcgaaga accttccgtc
     6181 gggcgatccg gaggcgtggg cggtcgccgc ctgcggcatg gacaggagag gcgacggctt
     6241 gcggtcgccg ccgagcggca gcctccagag ttgggcgcgg cccgtggcgc cctgggactc
     6301 gtacacgagc cacgccccgt ccggggagat gaagtcgccg tagttgtcga gggtcgtcgc
     6361 gaggagcggc tcgtctcctc ccgttccgtc gagagggcgg cggtagagac ccatgcgctc
     6421 ctcccggttc gacgagaagt agaggtactt cccgtccggc gaggcgatgc ccgtgttgtt
     6481 ggacttgggc ccgaacgtga gccgcgtcca cgcgccgcgc gcaaggtcca cctgccagag
     6541 gtccgggagg tccgtgcgcc ggtccgtcac cgtgatcccg agacgctgcc cgccgacgag
     6601 ccagaacggt tccgacatca cgccggcctc accgacggtg ccgagaggct ttccggaccg
     6661 gtcgaaccac gtgagcttct ggcgggggtt cgcgcgacgc ctgtaggcga gcgtgccgtc
     6721 cgcggcggaa aacgccgcga ggcccgtcca cccgctgtcg ccttccacga cgatcccgtc
     6781 gacgacgagc ccgggctcgc cggacagctt gcggccgtcg aacttctgtg aatacagcgc
     6841 gttctcgcgg acgtagagga ggcggcccgc ttcgtactcc gcttttccgc gcgtcgaaac
     6901 gacgaacgtg tcgtcggacg aatcgagaga cgccacgcgc gtacccgagc gcgccgggtc
     6961 gtcgctcaag gaaagaaaga cgaaacgggc accgtccgga aggaacttcg gccaccggtg
     7021 cgtgtagtcc ttcggaccga gcgtcgtcac cctctcgggg attccccccg acgcgctcac
     7081 gcggtggaga ggcacgcgta tcccgggagc gaagacgatg atcccccgcg gagaccagct
     7141 tccgccccgg ccgtcggtga ccgggcagat cgtcagcggc ggccccccgg ccgtggcgac
     7201 gcgcgcgaga cggtcgcccg tgaagtaggc gatggaagcg ccgtccggcg accagaacgg
     7261 gaatcgggcg ccttcggtgc ccgccaggac ctgggcgccg atggtgtcga acgagcgcac
     7321 gtagaggcgg tcgacgtcct tctcgtcggt cgcgacgaag gcgagccgcc ggccgtcggg
     7381 cgagagcgcc atcgagttgc cgaacgtcgc cccctccggg acgcgcacct cgaactggac
     7441 gacgggcgca ggtgcgcgcg ggacgcgagc gcgcagcgcg agcgcccccg cggcggccgc
     7501 gaacgcggcc gcgatcgccc aggaaagata gagggatttt ctgctccctc tctcacgact
     7561 cgtgcgttct gcgcctgctc ccgtgtggct cggaaccgcc tgtttttctt cgaaggtgaa
     7621 cgtgcccgaa gcgccgggcg cgctcatttc ctcgagttcg aggcgcgcat ccgcgatgtc
     7681 gtgaagacgg gcgcgtgcat cccgctgcag acaccttcta agaattcttc ttacagcggc
     7741 cggcgtctgt tccggcagcg ccgcccagtc cggctctccg cgcaggatcg ccgcgagcgt
     7801 gtcggagacg gtctcgccct cgaaggcgcg cttgcccgcc agcatctcgt acagcacgca
     7861 cccgaacgcc cagacgtccg tccgcttgtc taccggcttg ccccgcgcct gctcgggcga
     7921 catgtacgcg gccgtcccga ggatcacgcc cgccgccgtg ccgcgcgccg tcagtgtcgg
     7981 cgagtacgag atcgacgggt tcgacaacgt cgcctcgctc tcgaaaatct tcgcgaggcc
     8041 gaaatcgagg agcttcacgc gcccgtccgg cgtgatcttc acgttcgcgg gcttgaggtc
     8101 ccggtggacg atccccttct cgtgcgccgc ctcgagcgcc tcggcgatct gccgggcgaa
     8161 agaaagagat tcttcgagcg gaagagggcc ccgggcgacg cgcaccgcga ggtcgtcgcc
     8221 ctccacgagc tccatcacga gaaggtggcg cgtcggggac gacgagggag aggaggagaa
     8281 agaagggatt tcttcgaatg agtaaagaac ggcgatcccc ggatggttca ggctcgcgag
     8341 catgcgagcc tcgcgctcga agcgccctct cctctcttca ctttcaaaga actcttccgg
     8401 cagtactttc agcgcgaccg ccctgtcgac gcgggtgtcc ctcgcccggt agacctctcc
     8461 catgcccccg gccccgagag gcgcaaggat ctcgtagggg ccgaggcgtg tgcctgaaga
     8521 aatcgtcatc ttggagttcg gtctcagtat acgggcgcgc ccgcgagccg tcagccgcgc
     8581 aggatcgcgg agaggaagct cgcgccggcg aggggcggga cgccgaggtg ctcggcgacc
     8641 gtcgccccga gatccgcgaa cgtcgcgcgc gtcccgacgt ccgctccggc gcggacacgc
     8701 ggtcccgcgg cgaccatcgg cacgaactcg cgcgtgtggt ccgtggagac gtcggacggg
     8761 tcgccgccgt ggtccgccgt gaccatgagg acgtccccct cgcggaggcc cgcgagcagg
     8821 gcgggaacga acgaatcgag ctcctcgagg gcgcgcgcgt agccctgcgg gtcgttccgg
     8881 tggccgaact tcgtgtcgaa gtcgttgagg ttcgcgaaga cgaagtcgtc gtcccgcccc
     8941 gagagggccg cgaccgtctt ctcgagcccg tcgcgattgg actccgtatg gacggccttg
     9001 gggacgccgc gtcccgagaa caggtcctcg atctttccga tcccgtagac gcgcttgccc
     9061 tgcgccgtca gctcgtcgag cagcgtcggg ccgtccggcg ggaccgtgta gtcgtgccgc
     9121 cggtgcgtgc gcgtgaacga gcccggcgcc ccgacgaagg gccgcgcaat gacccggccc
     9181 acgcggtgct tgccgtcgag catgtcgcgg gcgatctcgc accagcggta gagcgtctcg
     9241 atcgggacga cgtcctcgtg cgccgcgatc tgaaagacgg agtcccccga ggtatagagg
     9301 atcgggcggc ccgtcttcat gtgctcttcg ccgtacagct tgatgacctc ggtccccgag
     9361 gcgggccggt tgaggaggac cttcttcccg accgcttttt cgaacggctc gacgacctcg
     9421 gggggaaacc cgtccgggta gagcgggaac gcgacgggca cggtgagccc catgagctcc
     9481 cagtggccgg tcgtcgagtc cttccccgcg gaagcctccg cgaggcggcc gaaggcgccc
     9541 gagggcgcag gcagcccggc gggcgcctcg ccgtacatcg tgtggaggag gccgagcttc
     9601 gtcaggtgcg ggatgacggg cctgccgtcg cgcaagatgt gctggagggt ccgcgcgccc
     9661 gcatcgccga acgcggcggc gtccggggcc tcgccgaccc cgagaccgtc gcagacgacg
     9721 acgatggccc gcctctgcac ctagagtccg gccagtttct gctgagcttc ggtgcgcagc
     9781 ggcgaatccg ggaagtccgt cacgagccgc tggtagaagc tcttcgcctc gacgttctgg
     9841 cccgtcttct cgtagagctt tgcgaccgag tagacgagaa cgtccttggg cagcggcgcg
     9901 tccgccgagt cgagatacct cttcgcgact tccagcgctt ccttcgagcg gccggcggcc
     9961 gactcggcgt cgagcgcagc gaccgcggcg actccggcgg cgaccgtgcc cttttcgctc
    10021 ttcgcgaacg cgcgggcggc gtcgagattc gtgcccgtcg cggcggagcc gccgtcgatc
    10081 ccgagcagca gcacggcggc cgcacggccc gacgcggaag acgggctgtc cttgacgagc
    10141 tcctggaacg cgctccgggc ggcggcggta cgctcggcgg cgtccttgta aaccttcgcg
    10201 cccggggccg ggctcggatc ggaggcgagc ggcgcgtcga agatcgcgag cgccgccgac
    10261 agccgcgaag cggcgcgatt gctcttccac tggaggccgc cccagatcgc gatcacgacg
    10321 acggcggccg ccgccccgat cgcgatccat cgggcggtct gggaacggtg gctcgagacg
    10381 aagtcgcgcg cgtcggtcag cgcgtgctcg agctggtcgg tacggagctg gtctttcgtc
    10441 agtctggcca tggcgggttc ggggccgcgc gtcagcagaa cgcggcgaat gccgcatccg
    10501 ggtggtcgag caggaagccc tcggtctcct tttcggggtc gtagtcgatc gacgcgccgt
    10561 cgaggcgcga gtaggaatag gggtcgagcc acacgacgag gccgccgccg gcgtccacgc
    10621 gccggccgtc ctcgagcggg cgctccgaga acgtgatccg gtagccggaa ccgccgcatc
    10681 cgaggacgtg ccggacgacg aggcaggtcc tgccgggctc ccagccctcg gccctgagag
    10741 cggcgagggc gcggtccgtc acccggatgc ggttcgactc ctccaccggg ctccttaccg
    10801 cttctttcgc ttcgcggcct tcttggcctt gcgcttcgga gcggcccgct tcgccctctt
    10861 cgtcttcttc acggccttgc gcgcgggctt tctcgcggcc ttcttcttcg gcgccgcctt
    10921 cttcttcgcg gccttccggg cggccttctt cttcggggcg gccttggcct tcttggcggc
    10981 ctttttcttc ggagcggctt tcttcttctt gggggcagct ttcttcttgg gggcagcggc
    11041 aggtgccggc ttggcggccg gagacg
//
LOCUS       DAOMFY010000118        11056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1839_length_11056_cov_6.154350, whole genome shotgun sequence.
ACCESSION   DAOMFY010000118 DAOMFY010000000
VERSION     DAOMFY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_11056_cov_6.154350"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1361)
                     /locus_tag="PLB01_10805"
     CDS             complement(93..1361)
                     /locus_tag="PLB01_10805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HQQ77836.1"
                     /translation="MTFAPWTDAMLAATRGMGDPLADAAVAALYEEAGGGRDGLKKLN
                     DFLFGLTFEDQFTHARLPGPVHAFAVQSADPPPWARPELVKAGQDFFYRNGLGATVLL
                     VCASLPECYVMQNGIHVLGMTTYLKDRPGLRILETSQMVRDAMLPGGLEPGAKGILSV
                     QKVRLLHAAVRHLILTTATGAGHDMGKVWTEARWNPEWGHPICQEDMAYTLQTFAYVV
                     LRGLKTMGAAYTPEEAEAFIHCWNVVGHVLGVDRSLLPEPEPGDGGPSRFARAEMLYH
                     AIRTRQAGRTDDGVAITGALVGFVENLIKDKVLPDLPLKFVRDVDLVRLPRLIMRDLL
                     GKETMQMLGVELSGGSGGDTRFFLLCLRFVYDVLQRVTGGTFLARASESLHIALTEEL
                     SKLPPGYKQTFLPASLRRTGPGAPPDPVDA"
     gene            complement(1358..3796)
                     /locus_tag="PLB01_10810"
     CDS             complement(1358..3796)
                     /locus_tag="PLB01_10810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HQQ77837.1"
                     /translation="MNRDKSDPSEEETFAPTPDRASPGVPASPAGADAPHDASGAVRY
                     EIEGLIAEGGMGSVFLARDPALGREVALKVAKGDDPTRRARFLDEMRILGQLEHPNVV
                     PLHSLVVNDRAPFCTMRYVRGRTLADVLGGLRGRDPETVARYSVTRLVQVLLQVTQAI
                     DFAHSRGVLHRDLKPSNVMLGEHGEVQVLDWGLAKVMERGDGKILASGTDHTRVGEVV
                     GTPGYMSPEQASSMPVDERSDVWALGVMMYEALTLERPFSGTNPIATLAAILRDDPKP
                     PRERAPGREIPVALERTCLKALAKSPDARHASVRELQDEMQAWLEAEADKVKRQESAG
                     LLALRGREILGDYRRQKAEIAAIGREVESLRGAFQPWQPVREKAPLTSAEDRLRNARA
                     RLATTGSNLVMTLAGALGHDDSHSEARAVMADYFFEQLLEAEETHDLRNVRYFGQIAA
                     AFDDGRHAAALKGDGSLALESDPPGAEVWLYEFVEKNLRLVPENERLLGKTPLAPVPL
                     PMGNYLVVLRARGRSDTRYPVFISRGRAWAGRVRMLTPAEIGEGFVHVPAGPYVLGGD
                     AALTGWSLPGRIAESEDFCIGVHPVTMGEYVDFLNDLVATDPAKATLHSPKKDVGGST
                     YLEVDETGRFSLPPRDLLGEEMHPRRPCVSITWHSAVAYAEWRSARDGRPYRLPTEFE
                     WEKAARGVDGRAFPWGDRFDASFCNSGESNPGRPRPLPVDSYETDVSVYGMRGAAGNA
                     RDWTATLLTLGSGDAAMVSSVIRGGAAEFAGSTEFSHMSRAAFRYVIPAFYLNPAISF
                     RLAFTPEPVKGDDR"
     gene            complement(3793..4566)
                     /locus_tag="PLB01_10815"
     CDS             complement(3793..4566)
                     /locus_tag="PLB01_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ77838.1"
                     /translation="MDRHVAVTGASSGLGEAIARAFGACGDRLTLVARRAGRLEAVAT
                     AFPGRARAVAFDLTSPDPAGWLPATEAAFGPVDVLVNAAGCQVIAPFADVSPAAADEM
                     TALNFRAPVSLVRAVLPGMRQRGSGAIVNVSSIGAAAPPPYMSDYVATKAALAAFSES
                     LAAELRGTGVDVLTVYPGPMDTPMGAYGLRTYGNAPSARLMPMGAPAGLARSVVRAVD
                     GHRSRLTYPRGYALMRWLPVPTAFLVGRFGPRPVVQEAR"
     gene            4672..6711
                     /locus_tag="PLB01_10820"
     CDS             4672..6711
                     /locus_tag="PLB01_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQQ77839.1"
                     /translation="MLYRLENVRKSFGAKDVLVDATWQHDPGRIVGLVGRNGAGKSTV
                     LKIVQGLVEPDGGKRYLAGGTTFASLEQAVEPEGEEPLRAFVARAQEPLLEVEREMRR
                     LEEAISAHGASGDAAKLQDLLHEHDEAREHFERAGGYEADSRIDRVLDGVGLPRALWD
                     RHVGELSGGQKHRAQIARLLLTDASVLLLDEPTNHLDVSGMEFLENWLVERKTSANLS
                     ALVVSHDRRFLNAVADRIVEVSNGRLEDYPGDYATYRKLKADRDRVRAKEAEKQRQMI
                     ERTEEFIRRNIAGQKTKQAQGRRKMLARLERIEAPREDANDVSIRFEEARVSGGRVLD
                     VKKVVPGYPETGPLTQPVTFLLRRGDRMALWGPNGCGKTTLLKNLARVLPPLSGSTVP
                     GVGVLVGYYDQEMADLSPAASALDVVMDLDPSMKEGEARDFLALFDFRGDEVYQKIAT
                     LSGGEKGRLSLARIVFGGANFLLLDEPTNHLDLDAREALEDALLEFEGAIVFVSHDRW
                     FVDRIATQILEIAPGGAVEVHDGNYSEMVKRKKEKDREKISLKGEEAGGASRAADARS
                     GAARKAGSQPGAAAQPSSPSRKSPSSSSSSDRAAERRRAKNQRLLFDLEEAISRLEEE
                     IRNLELSLADPDVYRDGEKARGVSAARDAARKALETKLWEWEETSRKLETYEVTA"
     gene            6708..7982
                     /locus_tag="PLB01_10825"
     CDS             6708..7982
                     /locus_tag="PLB01_10825"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HQQ77840.1"
                     /translation="MTAPAGPPRTASILAVGSELLGTTRLDTNSLFLTGRMEALGIST
                     VRKACVGDGWDDLLAEIAAALERAPLLVTSGGLGPTDDDRTKEAVAAVLGRTLVRDEE
                     ILLKLKERFRKRGYEMPEVNAKQADVIEGATVLGNRRGSAPGFLVEQGGKIVVLLPGV
                     PHELKAMWDDAAAPLLARTAAAGLHRRILKVAAIPESVVETLIKPVYAAHPDVPITIL
                     AAPGEVQLHFAARGAPEEAGALLDGIEAAFQTALAGEIFGRDDETLEDVVGDLLRRER
                     KTLALAESCTGGLLAGRLTDVAGSSDYFLGSAVTYANAAKADLVGVSAGTLERFGAVS
                     EETAREMALGARVRFGASIGLSVTGIAGPGGGTPEKPVGTVHLALDDAGGTRLHQRLL
                     MPGDRALVRRWSTSAALSMLRHHLIGSTGWRP"
     gene            7979..8578
                     /gene="plsY"
                     /locus_tag="PLB01_10830"
     CDS             7979..8578
                     /gene="plsY"
                     /locus_tag="PLB01_10830"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQQ77841.1"
                     /translation="MTRELLALAAAYLLGSVPFAIVLVRVFKGVDVRTQGSGNAGATN
                     VLRTAGKGLAVGTMLLDVGKGVAAVLLMRSVTLDPRWLGAAAVAAVLGHVFPVWFSFR
                     GGKGVATAIGSFGVLAPGAVLAVVGAFILVVAATRFVSLGSITAACLLPLAMRLLIHA
                     PDAEVVAAATVTLILVVSHRANIKRLLAGTERRLGRRDS"
     gene            8575..9573
                     /locus_tag="PLB01_10835"
     CDS             8575..9573
                     /locus_tag="PLB01_10835"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQQ77842.1"
                     /translation="MTQIAIVGTGAWGTALAVHAANAGHDVRLLGRRSDVVERMLATR
                     QHPALKGAPPIPERVRVTTDPADAFPGAAAVLWSVSVQATAAELARLKDVLPAVPLVA
                     TSKGIEIGTRRRTTEIMTFVTGRTAGLAALSGPTFAAEVARALPAAAVVASPDPEASR
                     FLQGLLSSPTMRLYRSKDVLGVEIAGAAKNVIAIAAGLVDGLRLGHNTRAAMLTRALA
                     EIRRLGTQLGGDPETFYGLAGVGDLILTATGDLSRNRRVGLALASGKTLPEAIAALGG
                     EVAEGVATAPALLELAAAAGVEMPISEMVRGILAGETSPADAVRRLMTRSLKEEKS"
     gene            9579..10613
                     /locus_tag="PLB01_10840"
     CDS             9579..10613
                     /locus_tag="PLB01_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77843.1"
                     /translation="MRLPPERPPAPGTQIVVPEGLSLFYARVATPADEPPPVPGVVDI
                     AVLDMHHGYANLGHASIVESLLNYAHDERARRSGNAPAVRVLSYDVRAGHAIPASVER
                     FPLIVGTGGPGALDPRENDGVSPGSQGVHEDPAWEAPLFRFFDAVIRTEGTALLGICH
                     SFGILSRWAGAARSELRPERKGGKSAGIVTNVLTDEAWAHPFFADYFAENGGAEVKVL
                     DSRLYDLLPTGNGFARPLAFECEPGGTRPGEAVTMLEFAHVLGSALPRVWGVNHHPEI
                     GDVGLQRERLFRLWENGGLTEAWFKERLSALDAWNASAAAEHGLQKTTSWTFERPLRL
                     HIARIFDERE"
     gene            complement(10617..10937)
                     /locus_tag="PLB01_10845"
     CDS             complement(10617..10937)
                     /locus_tag="PLB01_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77844.1"
                     /translation="MSCKEHGEHAHRHGDGCGHKAVRHGDHADYLHDGHLHAPHEGHA
                     DEHRLDVSRRRPGACAPVECTCPDGHASAGHAAVPHGDHVDYDVNGALHHPHGSHCDL
                     HGKL"
BASE COUNT         1561 a         3885 c         4061 g         1549 t
ORIGIN      
        1 agtgggagcg ggagccggcg gggcctgctg cgcgcgggcg gcgcccgcga acgcgagcgc
       61 ggcggctccg agcggagcga agaagcgccg cgtcacgcgt ccactgggtc cggcggggcg
      121 ccgggacccg tgcgccggag cgaggccggc aggaacgtct gcttgtatcc cggcgggagc
      181 ttcgaaagct cctccgtgag ggcgatgtgg agcgattcgg atgcgcgcgc gaggaaggtg
      241 ccgcccgtga cgcgctggag gacgtcgtag acgaagcgga ggcagagaag gaagaaccgc
      301 gtgtcgccgc cgctcccgcc cgagagctcg acgccgagca tctgcatcgt ctccttcccg
      361 aggaggtccc gcatgatgag gcgcggcagc cggacgaggt ccacgtcgcg gacgaacttc
      421 agcgggaggt cggggagcac cttgtccttg atgaggttct cgacgaatcc gacgagcgcg
      481 ccggtgatgg cgacgccgtc gtccgtccgg cccgcctggc gcgtgcggat cgcgtgatag
      541 agcatctcgg cgcgcgcgaa gcgcgaaggg ccgccgtcgc cgggctcggg ctcggggagg
      601 aggctgcggt cgacgccgag cacatggccg acgacgttcc agcagtggat gaacgcctcg
      661 gcctcctcgg gcgtgtaggc cgcccccatc gtcttcaggc cgcgaaggac gacgtacgcg
      721 aacgtctgga gcgtgtacgc catgtcttcc tggcagatcg ggtgccccca ctcggggttc
      781 cagcgggcct cggtccagac cttgcccatg tcgtggccgg cgcccgtcgc ggtcgtgaga
      841 atgaggtggc gcacggccgc gtggaggagg cgcaccttct ggacggacag gattcccttg
      901 gcgccgggct cgaggccgcc ggggagcatc gcgtcgcgga ccatctgcga cgtctcgagg
      961 atgcgcaggc ccgggcggtc cttcaggtac gtcgtcatcc cgagcacgtg gatcccgttc
     1021 tgcatcacgt agcactcggg caggcttgcg cacacgagga gcaccgtggc tccgaggccg
     1081 ttccggtaga agaagtcctg gccggccttc acgagctccg gacgggccca gggcggcgga
     1141 tccgcgctct ggacggcgaa cgcgtgcacg ggtccgggga gccgcgcgtg cgtgaactgg
     1201 tcctcgaacg tgaggccgaa caggaagtcg ttcagcttct tcagcccgtc acggccgccg
     1261 ccggcctcct cgtagagcgc ggccacggcc gcgtcggcga gagggtctcc catgccgcgc
     1321 gtcgcggcga gcatggcgtc ggtccacggt gcgaacgtca tcggtcgtcc cctttcacgg
     1381 gctccggcgt gaacgcgagc cggaagctga tcgccgggtt gaggtagaac gcggggatca
     1441 cgtagcggaa cgcggcgcgg ctcatgtggc tgaactccgt cgagcccgcg aactccgcgg
     1501 cgccgccgcg gatgacgctc gacaccatcg cggcgtcgcc ggatccgagc gtgagaagcg
     1561 tcgccgtcca gtcccgtgcg ttgcccgcgg ctccgcgcat gccgtagact gagacgtccg
     1621 tctcgtacga gtcgaccgga aggggccgcg ggcggcccgg gttgctctcg cccgagttgc
     1681 agaagctcgc gtcgaaccgg tcgccccacg ggaacgcgcg gccgtccacg ccccgggccg
     1741 ccttctccca ctcgaactcc gtcgggaggc ggtacggccg cccgtcgcgc gccgagcgcc
     1801 actccgcgta ggcgacggcg gaatgccacg tgatgctcac gcacggccgg cgcggatgca
     1861 tttcctcgcc gagaaggtcg cgcggcggga gcgagaagcg gcccgtctcg tccacctcga
     1921 gataggtgct gccgccgacg tccttcttgg gggagtggag cgtggccttc gccgggtcgg
     1981 tcgcgacgag gtcgttcagg aagtcgacgt actcgcccat ggtgacggga tgcacgccga
     2041 tgcagaagtc ctcgctctcg gcgatccgcc ccgggaggct ccagccggtg agcgcggcgt
     2101 cgccgccgag gacgtacggc cccgcgggga catggacgaa gccctcgccg atctcggcag
     2161 gcgtgagcat ccgcacgcgg cccgcccacg cgcgcccgcg cgagatgaag acggggtagc
     2221 gcgtgtccga gcggccccgc gcgcggagga cgacgaggta gttccccatc ggaagcggca
     2281 cgggggcgag aggcgtcttc ccgagcagcc gctcgttttc ggggacgagc ctgaggttct
     2341 tctccacgaa ctcgtagagc cacacctcgg cgcccggagg gtccgactcc agggcgaggg
     2401 agccgtcgcc cttcagcgcg gccgcgtggc gcccgtcgtc gaaggcggcc gcgatctgcc
     2461 cgaagtaccg gacgttccgg aggtcgtggg tctcctcggc ctcgaggagc tgctcgaaga
     2521 agtagtccgc catgaccgcc cgcgcctcgg agtgactgtc gtcgtggccg agcgcgccgg
     2581 cgagcgtcat gacgaggttc gagcccgtgg tcgcgagccg ggcgcgcgca ttgcgcaggc
     2641 ggtcctcggc cgaggtgagc ggcgccttct cacgcacggg ctgccagggc tgaaaggcgc
     2701 cgcgcaggga ctccacctcg cgcccgatcg cggcgatctc cgctttctgg cggcggtagt
     2761 ccccgaggat ctcgcggccg cgcagggcca ggaggccggc gctctcctgg cgcttgacct
     2821 tgtccgcctc ggcctcgagc cacgcctgca tctcgtcctg cagctcgcgc acggaagcat
     2881 ggcgggcgtc gggcgacttc gcgagggcct tgaggcacgt gcgctcgagc gcgaccggga
     2941 tttcgcggcc cggcgcgcgc tcgcggggcg gcttcgggtc gtcgcggagg atggccgcca
     3001 gcgtcgcgat cgggttcgtt ccggagaagg gccgctcgag cgtgagggcc tcgtacatca
     3061 tgacgccgag cgcccagacg tcggagcgct cgtcgaccgg catggacgac gcctgctcgg
     3121 gcgacatgta gcccggggtc ccgacgactt cgccgacgcg cgtgtgatcc gtgcccgaag
     3181 cgaggatctt cccgtccccg cgctccatca ccttcgcgag accccagtcg aggacctgga
     3241 cctcgccgtg ctcgccgagc atgacgttcg agggcttgag gtcgcgatgg agcacgccgc
     3301 gcgaatgcgc gaagtcgatc gcctgtgtga cctggagcag cacctgcacg agccgcgtga
     3361 cggagtaccg ggcgacggtc tccgggtcgc gcccgcgcag gccgccgagc acgtcggcga
     3421 gcgtgcggcc ccggacgtag cgcatcgtgc agaaaggggc gcggtcgttc acgacgagcg
     3481 agtggagcgg aacgacgttc gggtgctcga gctggccgag gatgcgcatc tcgtcgagga
     3541 agcgcgcgcg gcgcgtgggg tcgtcgccct tggccacctt gagcgcgacc tcccgcccga
     3601 gcgcggggtc gcgcgcgagg aacaccgagc ccatgccgcc ctcggcgatc aagccctcga
     3661 tttcgtaacg gaccgcgccc gacgcgtcgt ggggcgcgtc ggcccccgcg ggcgacgcgg
     3721 gaacgccggg ggacgcccgg tccggcgtcg gggcgaacgt ttcctcttcg gagggatcgc
     3781 tcttgtctcg gttcaacgcg cctcctgaac cacgggccgg gggccgaacc gccccacgag
     3841 gaaggcggtc ggaacgggca gccaccgcat gagcgcgtag ccgcgggggt acgtgaggcg
     3901 cgagcggtga ccgtcgacgg cgcgcacgac ggaccgcgcg agacccgcgg gggcgcccat
     3961 cggcatcagg cgcgcggaag gcgcgttccc gtacgtgcgc agcccgtacg cgcccatggg
     4021 tgtgtccatg ggtcccgggt agacggtgag gacgtcgacg cccgtgccgc gcagctcggc
     4081 ggcaagcgac tcggagaacg cggcgagcgc ggccttcgtc gcgacgtagt cgctcatgta
     4141 cggaggcggg gccgccgctc cgatcgacga gacgttcacg atcgcgcccg agccccgctg
     4201 tcgcatgccg ggcaggaccg cccgcacgag gctcacggga gcccggaagt tcaacgccgt
     4261 catctcgtcg gccgccgcgg gtgaaacgtc cgcgaagggc gcgatgacct ggcagcccgc
     4321 ggcgttcacg aggacgtcca cggggccgaa ggcggcctcg gtcgccggaa gccaaccggc
     4381 ggggtccggg gacgtgaggt cgaatgcgac ggcgcgagcg cgcccgggga aggcggtcgc
     4441 gacggcctcg agccggccgg cgcggcgcgc gacgagggtg aggcggtcgc cgcaggcgcc
     4501 gaaggcccgg gcgatcgcct cgccgaggcc ggaagatgct cctgtgacgg cgacgtgccg
     4561 gtccatttcc ctccccggtc gtatcgaaat ccgacgctgc agccgggatg gtaccagccg
     4621 gagcgactcg ttctcgaccg cgcgccccga cccttcgttt atcctcgcgc ggtgctgtac
     4681 cggctggaaa acgtccgcaa gagcttcgga gccaaggacg tcctcgtgga cgccacgtgg
     4741 cagcacgatc ccggccggat cgtgggtctc gtcgggcgga acggggcggg gaagtcgacc
     4801 gtcctgaaga tcgtccaggg cctcgtcgag cccgacggcg gaaaacgcta tctcgcaggc
     4861 gggacgacgt ttgcctcgct cgagcaggcg gtcgagccgg agggagagga gccgctccgc
     4921 gccttcgtcg cgcgggcgca ggaaccgctg ctcgaggtgg aacgggagat gcggcgcctc
     4981 gaggaggcga tctcggccca cggcgcctcc ggggatgccg cgaagctcca ggacctcctc
     5041 cacgagcacg acgaggcccg cgagcacttc gagagggcgg gcggctacga ggccgactct
     5101 cgcatcgacc gcgtgctcga cggcgtgggc ctgccgcgcg ccctctggga tcggcacgtc
     5161 ggcgaactct cgggcgggca gaagcaccgc gcccagatcg cgcgtctcct cctgacggac
     5221 gcctccgtgc tcctcctcga cgagcccacg aaccacctcg acgtctcggg gatggagttc
     5281 ctcgagaact ggctcgtcga gcgcaagacg tcggcgaacc tctcggctct cgtcgtctcg
     5341 cacgaccggc gtttcctgaa cgccgtcgcg gaccgcatcg tcgaggtttc caacggccgg
     5401 ctcgaggact atccgggcga ctacgcgacg taccggaagc tgaaggccga tcgcgaccgc
     5461 gtgcgcgcga aggaggccga gaaacagcgg cagatgatcg agcggaccga ggagttcatc
     5521 cgccgcaaca tcgcgggcca gaagacgaag caggcccagg gtcggcggaa gatgctcgcg
     5581 cgcctcgaac gcatcgaggc gccgcgcgag gacgcgaacg acgtctcgat ccgtttcgag
     5641 gaggcgcggg tctccggcgg gcgcgtcctc gacgtgaaga aagtcgtgcc cggctacccg
     5701 gagacgggtc ccctcacgca gcccgtcacg ttcctcctgc ggcggggcga ccggatggcg
     5761 ctctgggggc cgaacggctg cggcaagacg acgctcctca agaacctcgc gcgcgtcctg
     5821 ccgccgctct cgggctccac cgtccccggc gtgggcgtcc tggtcgggta ctacgaccag
     5881 gagatggccg acctctcgcc ggccgcgtcc gcgctcgacg tcgtgatgga cctcgacccc
     5941 tcgatgaagg agggcgaggc gcgcgacttc ctcgcgctct tcgacttccg gggcgacgag
     6001 gtctaccaga agatcgcgac gctctcgggc ggggagaagg gccggctctc cctcgcgcgg
     6061 atcgtcttcg gcggcgcgaa cttcctcctc ctcgacgagc cgacgaacca cctcgacctc
     6121 gacgcgcgcg aggcgctcga ggacgcgctc ctcgagttcg agggcgcgat cgtcttcgtg
     6181 tcgcacgacc gctggttcgt cgaccgcatc gcgacgcaga tcctcgagat cgcgcccggc
     6241 ggcgcggtcg aggtccacga cgggaactac tcggagatgg tgaagagaaa gaaggagaaa
     6301 gacagagaga agatttcttt gaaaggtgaa gaggccggcg gcgcatcccg cgcggccgac
     6361 gcgcgctccg gcgcagcccg caaagccgga tcgcagcccg gcgctgcggc acagccctct
     6421 tcaccttcca ggaaatctcc ctcttcttct tcctcttcgg accgcgccgc cgagaggcgg
     6481 cgcgcgaaga accagagact tctcttcgac ctcgaggagg cgatctcccg cctcgaggag
     6541 gagatccgga atctcgagct ctccctcgcc gatcccgacg tctaccggga cggtgagaag
     6601 gcgcggggcg tttcggccgc ccgcgacgcg gcccgcaagg cgctcgagac gaagctgtgg
     6661 gagtgggaag agacgtcgcg caagctcgag acatacgagg tgacggcgtg acggcgcccg
     6721 cgggcccccc gcgcacggcc tcgatcctcg ccgtcgggag cgagcttctc gggacgacgc
     6781 gcctcgacac gaattcgctc ttcctgacgg gccgaatgga ggccctcggc atctccacgg
     6841 tccggaaggc ctgcgtcgga gacggctggg acgacctcct cgccgagatc gccgccgcgc
     6901 tcgagcgcgc gcctctgctc gtgacctccg gaggcctcgg cccgaccgac gacgaccgca
     6961 cgaaggaagc cgtcgcggcg gtgctcggcc gcacgctcgt gcgggacgag gagatcctcc
     7021 tcaaactcaa ggagcgcttc cggaagcgcg gctacgagat gcccgaggtg aacgcgaagc
     7081 aggcggacgt catcgagggc gcgaccgttc tcgggaaccg ccgcgggagc gcgccgggct
     7141 tcctcgtcga gcagggcggg aagatcgtcg tcctgctgcc gggcgtcccg cacgagctga
     7201 aggcgatgtg ggacgacgcg gccgctccgc tcctcgcgcg gacggcggcc gccggcctgc
     7261 accggcgcat cctcaaggtg gccgcgatac cggaaagcgt cgtcgaaacg ctcatcaagc
     7321 cggtctacgc ggcgcatccg gacgtgccga tcacgatcct cgcggcgccc ggcgaggtgc
     7381 agctgcactt cgcggcgcga ggcgcgcctg aggaagccgg cgccctcctc gacgggatcg
     7441 aagccgcgtt ccagacggcg ctcgcgggcg agatcttcgg gcgggacgac gagacgctcg
     7501 aggacgtggt cggggacctc ctgcgccggg aacggaagac gcttgcgctg gcggagtcct
     7561 gcacgggcgg cctgctcgcc gggcgcctca cggacgtcgc gggctcgtcg gactacttcc
     7621 tcggttcggc cgtgacgtat gcgaacgccg cgaaggcgga cctcgtgggc gtctcggccg
     7681 gaacgctcga gcgcttcggc gcggtctccg aggagaccgc ccgcgagatg gcgctcggcg
     7741 cgcgggtccg cttcggcgct tcgatcggcc tctccgtcac cggaatcgcg gggccggggg
     7801 gcggcacccc cgaaaagccg gtcgggacgg tccacctggc cctcgacgac gccggcggaa
     7861 cgcgcctcca ccagagactc ctgatgccgg gcgaccgcgc gctggtccgg cgctggagca
     7921 cctcggcggc gctctcgatg ctgcgccacc acctgatcgg gtcgacgggg tggcgcccgt
     7981 gacgcgggag ctcctcgcgc tagccgccgc gtacctcctc ggctccgtgc cgttcgcgat
     8041 cgtcctcgtg cgcgtcttca agggagtcga cgtgcggacg cagggctcgg gcaacgcggg
     8101 cgcgacgaac gttttgcgca cggccgggaa gggcctcgcg gtcggcacga tgctcctcga
     8161 cgtcggcaag ggcgtcgccg cggtcctcct catgcggtcc gtcacgctcg acccgcggtg
     8221 gctcggcgcg gccgcggtcg ccgcggtcct cggccacgtc ttcccggtct ggttttcgtt
     8281 ccgcggcggc aagggcgtcg cgactgcgat cggcagtttc ggcgtcctcg ccccgggagc
     8341 ggtcctcgcg gtcgtggggg ccttcatcct cgtcgtcgcg gcgacgcgct tcgtttccct
     8401 cggctcgatc acggccgcgt gcctcctgcc gctcgcgatg cggctcctca ttcacgcccc
     8461 ggacgccgag gtcgtcgccg ccgccacggt gacgctgatc ctcgtcgtct cgcaccgggc
     8521 caacatcaag cggctcctcg cgggcaccga acgccgcctc ggacggaggg attcgtgacg
     8581 cagatcgcca tcgtgggcac gggcgcgtgg gggacggcgc tcgccgtcca cgccgccaac
     8641 gccgggcacg acgtccggct gctcggccgg cgcagcgacg tcgtcgagcg gatgctggcg
     8701 acccgccagc atcccgcgct caagggcgcg ccgccgatcc cggagcgggt ccgcgtgacg
     8761 acggatccgg cggacgcgtt tcccggcgct gcagccgtgc tctggtccgt gtcggtccag
     8821 gcgacggccg ccgagctcgc gcgcctgaag gacgtcctcc cggcggtccc gctcgtcgcg
     8881 acctcgaaag ggatcgagat cgggacgcgc cggaggacga ccgagatcat gacgttcgtg
     8941 acgggccgga ccgccggcct cgcggcgctc tcggggccca cgttcgccgc cgaggtggcg
     9001 cgcgcgctgc ccgcggcggc cgtcgtcgcg tcacccgacc cagaggcgtc ccgcttcctg
     9061 caggggctcc tgtcgtcgcc gacgatgcgc ctctaccggt ccaaggacgt cctcggcgtc
     9121 gagatcgcgg gagccgcgaa gaacgtgatc gcgatcgcgg cgggcctcgt ggacggcctc
     9181 aggctcggcc acaacacgcg cgccgcgatg ctcacgcgtg cgctcgccga aattcggcgg
     9241 ctcgggaccc agttgggcgg cgaccccgag accttctacg gcctcgcggg cgtcggcgac
     9301 ctcatcctga cggcgacggg cgacctctcg cgtaaccggc gtgtcggtct cgcgctcgcg
     9361 tcgggaaaga cgctccccga ggccatcgcg gcgctcggcg gcgaggtggc cgagggcgtc
     9421 gcgacggctc ccgcccttct cgagctcgcc gccgctgcgg gcgtcgaaat gccgatctcc
     9481 gagatggtcc gtggcatcct cgccggcgag acgtcgccgg ccgacgccgt tcggcggctc
     9541 atgacgcgct cgctgaagga ggaaaagtcc tgaacgaggt gaggctcccg cccgagcgac
     9601 cgccggctcc cggcacgcaa atcgtcgtcc cggaagggct gtcgctcttc tatgcgcgcg
     9661 tggcgacgcc ggcggacgag ccgccgcccg tgcccggcgt cgtggacatc gcggtcctcg
     9721 acatgcacca cggttacgcg aatctcggcc acgcctcgat cgtcgagagc ctcctcaact
     9781 acgcgcacga cgagcgcgcg aggcggagcg ggaacgctcc cgccgtgcgc gtcctcagct
     9841 acgacgtgcg cgcggggcat gcgatccccg cgtccgtcga gcggttccct ctcatcgtcg
     9901 ggacgggcgg tcccggcgcg ctcgatccgc gggaaaacga cggcgtgtcg ccgggctcgc
     9961 agggcgtcca cgaggatccc gcatgggaag cgccgctctt ccggttcttc gacgccgtga
    10021 tccgcaccga gggcaccgcc ctcctcggaa tctgccattc cttcgggatc ctgtcgcgct
    10081 gggcgggcgc ggcgcgctcc gagctgcgcc cggagcgtaa gggcggcaag agcgccggaa
    10141 tcgtcacgaa cgtcctcacc gacgaagcgt gggcgcaccc gttcttcgcc gactacttcg
    10201 ccgaaaacgg cggcgccgag gtcaaggtcc tcgacagcag gctttacgac ctcctgccga
    10261 ccggaaacgg gttcgcgcgc cctctcgcgt tcgagtgcga gcccggcgga acccggcccg
    10321 gcgaggccgt cacgatgctg gagttcgccc atgtcttggg aagcgcgctg ccccgggtgt
    10381 ggggcgtgaa ccaccacccg gagatcggag acgtcggcct ccagcgcgag cggctcttcc
    10441 ggctctggga gaacggcggc ctgaccgagg cctggttcaa ggaacgcctg agcgcgctcg
    10501 atgcgtggaa cgcctcggcg gcggccgagc acgggctcca gaagacgacg tcctggacgt
    10561 tcgagcggcc cctccgcctc cacatcgcgc ggatcttcga cgaaagggag taggagctag
    10621 agcttcccgt gcaggtcgca gtgcgacccg tgcgggtggt ggagcgcgcc gttcacgtcg
    10681 tagtccacgt ggtccccgtg cgggaccgcc gcgtggccgg cgctcgcgtg cccgtccggg
    10741 cacgtgcact cgaccggcgc gcaggcgccg gggcgccttc ggctcacgtc caggcggtgc
    10801 tcgtcggcgt ggccttcgtg gggggcgtgc aggtgcccgt cgtggaggta gtccgcgtgg
    10861 tcgccgtggc ggacggcctt gtggccgcag ccgtcgccgt ggcggtgggc gtgctccccg
    10921 tgctccttgc agctcacgcc ggcatcgtct cgcgtcgcgg cggcgcggtc aaccgtggga
    10981 taatcgtcta gatgcaattg cgttgcatga aggcgggcat cctcctcgcg gggtccgtcg
    11041 ccgcgggcgc cgcggg
//
LOCUS       DAOMFY010000119        10987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1850_length_10987_cov_7.303879, whole genome shotgun sequence.
ACCESSION   DAOMFY010000119 DAOMFY010000000
VERSION     DAOMFY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1850_length_10987_cov_7.303879"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLB01_10850"
     CDS             <1..675
                     /locus_tag="PLB01_10850"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HQQ77845.1"
                     /translation="FRRDRALLVVFAVTGIGALAALLGADVATGRDPGEVAAARWAAA
                     VPEMLEYYRSSGWGESSVATVARLFEILRVALRDQLPGIVLAIVMGHAAVVVYGFARA
                     SGIAAADWQEPSFSRFRTPLAAAAVFVPAGLLAALGGPDLRRPAVDVLLPLAVLFFLR
                     GLAIIRALLDRGRAGLLGRALVYALVFQMPFPFLLALGGLFDEFVDVRARLDRMPPAE
                     VGPPDF"
     gene            715..1164
                     /gene="rplI"
                     /locus_tag="PLB01_10855"
     CDS             715..1164
                     /gene="rplI"
                     /locus_tag="PLB01_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQQ77846.1"
                     /translation="MKLVLTDDVKGLGHRGDVVTVAAGYGRNFLLPKALAYPATPGNV
                     KRLEQEKKRYDARVAKEKDQAEAVAKSVEGTKIVLHKKAGEGDHLYGSVTASEVADGL
                     AAKGITVDKRRVELEEPIKRLGTHTVHVKLHKDVTVAVIVEVLPLSQ"
     gene            1206..3782
                     /locus_tag="PLB01_10860"
     CDS             1206..3782
                     /locus_tag="PLB01_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77847.1"
                     /translation="MAIANGTRLGPYEVLAPLGAGGMGEVYRARDPKLARDVAVKVLP
                     EDFLEGEERKERFEREAKLLAALNHPNIAAVYSFEEIPGSSPNSLRPVLVMELLEGET
                     LRARLAGGALPVRKAVDYGAQIARGLAAAHGKGVVHRDLKPENLFVTKDGRVKILDFG
                     LAKLVDAGAGSGSTNLPTQSKGTEPGAVLGTLGYMSPEQLRGLPADHRTDLFSFGAVL
                     YEMLSGRKAFRGDTAADTVSAILREEPEELSVTNRSIPPALERIVRHCLEKSPDERAH
                     SAHDLAFDLESFSTASSGTTLASAPRVEIRRPSSRALFGAAVCLGAGVLGGRLLWKKP
                     PDAPPTFHRLTFRRGNVLRARFAPDGRTIVYGAAWGGAPSEIFTVRTESPESRPLGFA
                     SATVLSVSSAGEVAVLSADGMKDNSGWGTLQRVPLSGGAPRTLLEEVTDADWTPDGKE
                     LAVARIGEAGGRRIELPPGRPIYRSESRITALRVSPDGGRVAFVDGDQKPVLRVVDRA
                     GKSQALAGPFEVFSGRFAWSPSGREVFYFGGADRQDVALRAVDLSGRVRTVYRTTGYL
                     FVHDVFPDGRMLLEHASSLYGLMFGRVGGSSEADLSWFDGSGIVAVAPAGTGILFNES
                     GEAVGGRPQAFFRKADGSPAVRLADGEALALSPDGGRVLIRSGRALAIAPIGAGTPVA
                     VASDQVRELRSAAFFPDGRRILLHGADADRKDRFWIVEPLAAPREASPPGVSGQAFLM
                     SPDGRSVVAWSAETSVLSLLPLDGGPARPLAGTENDDPLGWTADSRSLYVRKNPGQTR
                     TITLLDVATLARRPWKDLAPPDPASVTFAPSVSFARDGSAYAYTYRRVLTSDLYVVEG
                     LK"
     gene            complement(3786..6203)
                     /locus_tag="PLB01_10865"
     CDS             complement(3786..6203)
                     /locus_tag="PLB01_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HQQ77848.1"
                     /translation="MSARRIAALAVLLVPVLLVAGLGVAAGYGLSRAIRIPRVSELAT
                     YRPDIVTEIRGADGSTIARFAIERRVLIERKDIPEVMVKALLAAEDARFYDHSGIDIV
                     RIGGALLKDIATRRLSQGASTLTQQLARAVFLSPEKTIARKVNEAFLTVEIERQFSKD
                     QILTMYLNQIPWGHGNYGVEAASRWYFGHAAKVLTLPEAALLAGIIQRPAYYSPVRNA
                     GAAKSRRDFVLRRMREEGYISEDQRAAAAAAPIEVSKGTRESTVGPYFCEEIRQYLEK
                     QYGESELYREGLRVDSTLDPRLQRWAEDALRWGLRRQDRRSGFRRPRNLVAEGLDPET
                     WKDPSWSDGASGGSPDVLTAVVLATSKTGAEVRVGDKRFALPAAAFRWTRAENPVKAV
                     SRGDVIQIETLTSDAGKSEAIVAQDPNVEGAAVILENATGAVRALVGGYDFNRSKFDR
                     AVQALRQVGSAFKPVVFLTAIEQGFTPADTVLDAPISITIDPKQEPWQPQNFEKKYNG
                     IITYQYALEHSVNLATVRVDLLVGTKTVLETARRLGVRQNLLAYPSLALGAFEVTPME
                     MASAYASIAAGGLVYKPAFIEKIRNADGITLEQWSPEPKEATSPASAYVLLGMMKGVV
                     QRGTAASAAKLGLNLAGKTGTTNEYSDAWFIGMTPKHTIAVWVGHDTRKPLAGGSHAQ
                     GAEVALPIFTKIVERMKAEGVVTATDDFEAPQGVVLVPVDEATGYRAAPGCTKTVLMA
                     FVNGTQPTEMCGDQPHAVANLPQYLQRAIYAPKRGETKGDEVTLTDAPKLAAPLPKPG
                     KEGGPPG"
     gene            6259..6723
                     /locus_tag="PLB01_10870"
     CDS             6259..6723
                     /locus_tag="PLB01_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77849.1"
                     /translation="MAEEPVPPAPAPLIPKLPTTLPFEDEAGRARRDGCLKWGLVGCA
                     GASVVLIVGMLFVLSNAKKLMGLAFEKIGDQVVAAAGPEVTPAEKERFRTALTAFAER
                     AKAGTAKPAAVQTWQSQVTGALADGRVTGDEMRSLTVWLETSGAAPPPAPAK"
     gene            6728..8710
                     /gene="uvrB"
                     /locus_tag="PLB01_10875"
     CDS             6728..8710
                     /gene="uvrB"
                     /locus_tag="PLB01_10875"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQQ77850.1"
                     /translation="MADFQVLSPLTPQGDQPRAIEQLVAGFREGREKQVLLGVTGSGK
                     TFTMAKVVEALNRPTLVLSHNKTLAAQLYQEFRSFFPKNRVEYFVSYYDYYQPEAYVP
                     QSDTYIEKETDINEEIDKLRIAATAALFERRDVLIVASVSCIYGLGSPESFGKMAIEI
                     EKGARYGMTWYLKRLVEAQYERTNMDLSRGTFRVRGDVLDVVPSYEELGLRVEFFGDE
                     IERITRFETVTGRNVGEIPRFELFPKSHYVTPEEVRKRALGSIEAELVVRLEELKEQN
                     KLLEAQRLEQRTRFDLEMMREIGYCAGIENYSRHLSGRKPGEPPPTLLDYFPSDYLTI
                     VDESHQTVPQVRAMYHGDQSRKKTLVEYGFRLPSALDNRPLQFEEWLERTGQRLFVSA
                     TPAPYELQETGGEVVEQVLRPTGLLDPPIFVRPVKGQVDDLLSSVKERTAKNERTLVT
                     TLTKRMAEDLTNYFLELGIRVRYLHSDVETLERVQIVGDFRRGVFDVLVGVNLLREGL
                     DLPEVSLVAVLDADKEGFLRSETSLIQTAGRAARNVNGIAVFYADKITDSMKRAMSET
                     ERRRKLQGDWNAAHGIVPETIVKSLDSPLLQMADLDYLEPSGVERRPKAKDAPSLDRE
                     IADLEKEMKVAAKELEFEKAAQLRDRIRELRQMKLF"
     gene            complement(8886..8962)
                     /locus_tag="PLB01_10880"
     tRNA            complement(8886..8962)
                     /locus_tag="PLB01_10880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8926..8928),aa:Arg,seq:cct)
     gene            complement(8974..10725)
                     /locus_tag="PLB01_10885"
     CDS             complement(8974..10725)
                     /locus_tag="PLB01_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77851.1"
                     /translation="MARKALPGLLLFAAVVTAFLPALAGGFIWNDDTYLTANRTLDGL
                     DGLRLIWTQPRANEQYYPMVFTSFWVEKRLWGLDPFGYHLVNVLLHAGSALLLWRLLA
                     RLGLRGAWLAAAAFALHPMCVESVAWVTERKNTLSLFLSLLAMHAYFESRDRREDEEK
                     TGDSLRKGKRRGSPPHSDAGPSARALTFIGFFFFVLALLAKTTASVVPPVLLVLVWWR
                     EGRVKAADARPLVPWFGAGIALGAHTAWLERTSVMAVGSEWTLSVADRIVLAGRTVFF
                     YLQKFLLPLDLSFIYPRWKVDAHAPLQWLPALGALVLLALAWRFRVRVGRGPFAFLLL
                     FGGVLFPAMGFFNVYPMRFSWVADHFAYQAVAVAAASIVCGAAAWTAGWSAKATRAAA
                     AFAVAAIALLGALTFRQGRIYRDEEALWSATLVKNPECFICHTNYGIWLMESGRDPEA
                     AAHLEASYRLKPDEVPTLLNLALIEERRGRTERAAALLRSALRVDPANTAALINLAKA
                     DARAGRVEEAIASYREALHLGSPGDHLAHNGLGAALMRQGQEAEAIEHFREALRLRPD
                     YEHARTNLERALASGRR"
     gene            10788..>10987
                     /locus_tag="PLB01_10890"
     CDS             10788..>10987
                     /locus_tag="PLB01_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77852.1"
                     /translation="MKKDERRTGERARAVIPIGVRIGERKLTAGCTLDAGPTGALILC
                     TENVPVGTELHVTNLQSDEWFT"
BASE COUNT         1655 a         3789 c         3869 g         1674 t
ORIGIN      
        1 ttccgccgcg accgggcgct cctcgtcgtc ttcgccgtga ccgggatcgg cgccctcgcg
       61 gcgcttctcg gcgcggacgt cgcgacgggg cgggacccgg gggaggtcgc ggccgcgcgc
      121 tgggccgcgg ccgtgcccga gatgctcgag tactaccgtt ccagcgggtg gggcgaatct
      181 tccgtcgcga cggtcgcccg cctcttcgag atcctccggg tggccctgcg cgaccagctg
      241 ccgggcatcg tcctcgcgat cgtgatgggg cacgcggccg tcgtcgtgta cggcttcgcc
      301 cgggcctccg gcatcgccgc ggccgactgg caggagccgt cgttcagccg gttccggact
      361 ccgctcgcgg ccgcggccgt gttcgttccg gccggcctcc tcgccgcgct gggcgggccg
      421 gacctccgcc ggccggccgt ggacgtcctg ctgccgctcg ccgtcttgtt ttttctgcgt
      481 ggtctggcca taatccgcgc tcttctcgac aggggaagag ccggccttct gggccgggcg
      541 ctcgtttacg cgctggtctt tcagatgccg tttcccttcc tcctggcgct cggggggctg
      601 ttcgacgagt tcgtcgacgt ccgggcgcgg ctcgaccgga tgcctccggc cgaggttggg
      661 ccgcccgact tctgagaccg gcgggaattc acgaacttgg atgaggagcc cccgatgaaa
      721 ctcgtactga ccgacgacgt gaagggcctc gggcaccggg gcgacgtcgt gaccgtcgcc
      781 gccggatacg gccgcaactt ccttctcccg aaggcgctcg cctatccggc cacgcccggg
      841 aacgtgaagc gcctcgagca ggagaagaag cgctacgacg cccgcgtcgc gaaggagaag
      901 gaccaggccg aggcggtcgc caagtccgtc gaggggacga agatcgtcct ccacaagaag
      961 gcgggcgagg gcgaccatct ctacggctcg gtgaccgcgt cggaagtggc cgacgggctc
     1021 gccgccaagg ggatcaccgt cgacaagcgg cgcgtcgagc tcgaggagcc catcaagcgc
     1081 ctcggaacgc acaccgtgca cgtcaagctc cacaaggacg tgacggtcgc cgtcatcgtc
     1141 gaggtccttc cgctctcgca gtaaatcccc ggtcccctcc tcccgtacaa tcgcgacccc
     1201 gtctcatggc gatcgcgaac ggaacccgtc tcgggcccta cgaggtcctc gccccgctcg
     1261 gcgcgggcgg gatgggagag gtctaccggg cgagagaccc gaagctcgcg agggacgtcg
     1321 cggtcaaggt actgcctgaa gatttcctgg aaggtgaaga gcggaaagag cgcttcgaga
     1381 gggaagcgaa gctgctggcc gcgctgaacc acccgaacat cgcggccgtc tattcattcg
     1441 aagagatccc cggttcttct ccgaattctc tgcgccccgt cctcgtcatg gagctgctcg
     1501 aaggcgagac gctgcgcgcg cgcctcgcgg gcggcgcgct gcccgtgagg aaggcggtgg
     1561 actacggggc ccagatcgcc cgcggcctcg cggccgcgca cgggaagggc gtcgtccacc
     1621 gcgacctcaa gccggagaac ctgttcgtca cgaaggacgg gcgcgtcaag atcctcgact
     1681 tcggcctcgc gaagctcgtc gacgccggcg ccggcagcgg cagcaccaat ctcccgaccc
     1741 agtcgaaggg gacggagccg ggagccgtgc tcgggacgct cggctacatg tcgcccgagc
     1801 agctgcgcgg cctgccggcg gaccatcgga ccgacctctt cagcttcggc gcggtcctct
     1861 acgagatgct ctcgggccgg aaggcgttcc gcggcgacac ggcggcggac acggtctcgg
     1921 cgatcctccg cgaggagccg gaggagctct cggtcacgaa ccgctcgatc ccgccggcgc
     1981 tcgagcgcat cgtccgccac tgtctcgaaa agagccccga cgagcgggcg cactccgcgc
     2041 acgacctcgc gttcgacctc gagtcgttct cgaccgcgtc ctccggcacc actctggcgt
     2101 ccgcgccccg ggtcgagatc cgccggcctt cgtcccgggc gctcttcggg gccgcggtgt
     2161 gcctcggagc gggcgtcctc ggaggacggc tgctgtggaa gaagccgccg gacgcgccgc
     2221 cgacgttcca ccggctcacg ttccgtcgcg ggaacgtgct gcgggcgcgc ttcgcgccgg
     2281 acgggcggac gatcgtctac ggcgcggcat ggggaggggc gccgtccgag atcttcacgg
     2341 tccggaccga gagtcccgag tcccggccgc tcgggtttgc gagcgcgacc gtcctctcgg
     2401 tttcgtcggc cggtgaggtc gccgtcctct ctgccgacgg gatgaaggac aattcgggct
     2461 gggggacgct ccagcgcgtc ccgctctcgg gcggcgcgcc gcgcacgctc ctcgaagagg
     2521 tcacggacgc cgactggacg ccagacggca aggagctcgc ggtcgcgcga atcggcgagg
     2581 cgggtggacg ccggatcgag ctgccaccgg ggcgtccgat ctaccgttcc gagtcgcgca
     2641 tcacggcgct ccgcgtctcg cccgatggag ggcgggtcgc gttcgtcgac ggcgaccaga
     2701 agcccgtcct gcgcgtcgtc gaccgcgccg ggaagtcgca ggcgctcgcg ggtcccttcg
     2761 aggttttctc tgggcggttc gcctggagcc cgtccggccg cgaagtcttc tacttcggcg
     2821 gagccgaccg gcaggacgtc gcgctccgcg cggtcgatct ctcgggacgc gtgcgcaccg
     2881 tgtaccgcac aaccgggtat ctcttcgttc acgacgtgtt tcccgacggg cggatgctcc
     2941 tcgagcacgc gtcgagcctc tacgggctca tgttcgggcg cgtgggcggc tcctcggagg
     3001 ccgatctctc ctggttcgac ggatcgggga tcgtcgcggt cgctccggca gggacgggaa
     3061 ttctcttcaa cgagtcgggc gaggccgtcg gagggcgtcc gcaggcgttc ttccgcaagg
     3121 ccgacggctc gcccgccgtg cgcctcgcgg acggcgaagc tctcgcactg tcgccggacg
     3181 gcggacgcgt gctcatccga tcgggacgag cgcttgcaat cgccccgatc ggcgccggga
     3241 cgccggtggc cgtcgcctcg gaccaggtcc gcgagctgcg ctccgctgcg ttcttcccgg
     3301 acggccgccg gatcctcctg cacggcgcgg acgcggaccg caaggaccgc ttctggatcg
     3361 tcgagccgct ggcggcgccg cgcgaggctt cgcctccggg tgtgagcggc caggcgtttc
     3421 tcatgtcgcc ggacggccgc tcggtcgtcg cgtggtcggc cgagacctcg gtcctttcgc
     3481 tccttccgct cgacgggggc cccgcgcgcc ccctggcggg cacggagaac gacgatcctc
     3541 tcgggtggac ggccgactcg aggtcgctct acgttcggaa gaacccgggg cagacgcgca
     3601 cgatcaccct gctcgacgtc gcgacgctcg cgcgccggcc gtggaaggac ctcgcgcctc
     3661 ccgatcccgc gagcgtgacg tttgcgccgt ccgtttcctt cgcccgcgac ggctccgcgt
     3721 acgcttacac gtaccgccgc gtcctgacgt cggacctcta cgtcgtcgaa ggcctgaagt
     3781 agccctcagc ccggcgggcc gccctctttc cccggctttg ggagcggggc cgcgagcttc
     3841 ggggcgtccg tgagcgtgac ctcgtctccc ttcgtctcgc cgcgcttcgg cgcgtagatc
     3901 gcgcgttgga ggtactgcgg gaggttcgcg acggcgtgcg gctggtcgcc gcacatctcg
     3961 gtcggctgcg tcccgttgac gaacgccatg agaacggtct tcgtgcagcc cggcgccgcg
     4021 cggtacccgg ttgcctcgtc gacgggcacg agaacgacgc cctgaggcgc ttcgaaatcg
     4081 tctgtcgcgg tcaccacgcc ctcggccttc atccgctcga cgatcttcgt gaagatcggg
     4141 agcgcgacct cggcgccctg cgcgtgcgag ccgcccgcca ggggcttcct cgtgtcgtgg
     4201 ccgacccaga ccgcgatcgt gtgcttcggc gtcatgccga tgaaccacgc atccgagtac
     4261 tcgttcgtcg tgcccgtctt gcctgcgagg ttcaggccga gcttcgccgc ggacgcggcc
     4321 gtcccgcgct gcacgacgcc cttcatcatc ccgaggagga cgtacgccga ggcgggcgac
     4381 gtggcctcct tcggctcggg cgaccactgc tcgagcgtga ttccgtcggc gttgcggatc
     4441 ttttcgatga acgccggctt gtagacgagc ccgcccgccg cgatggaggc gtacgcggac
     4501 gccatctcca tcggcgtcac ctcgaacgcg ccgagcgcga gcgaggggta ggcgaggagg
     4561 ttctgccgga ccccgaggcg ccgcgccgtc tcgaggaccg tcttcgtccc gacgagcagg
     4621 tcgacgcgca ccgtcgcgag gttgaccgag tgctcgaggg cgtactggta cgtgatgatc
     4681 ccgttgtact tcttttcgaa gttctgcggc tgccagggct cctgcttcgg gtcgatcgtg
     4741 atcgagatcg gcgcgtccag gaccgtgtcc gccggcgtga agccctgctc gatcgccgtc
     4801 aggaagacga cgggcttgaa cgccgacccg acctgccgaa gcgcctggac ggcgcggtcg
     4861 aacttcgagc ggttgaagtc gtagcccccg acgagcgccc gcacggcgcc cgtggcgttc
     4921 tcgaggatga ccgcggcgcc ctcgacgttc gggtcctgcg cgacgatcgc ctcggacttg
     4981 cccgcgtcgc tcgtcagcgt ctcgatctgg atgacgtcgc cgcgcgagac ggccttgacg
     5041 ggattctcgg cgcgtgtcca gcggaaggcc gcggccggaa gcgcgaagcg cttgtcgccg
     5101 acacgcacct cggcgcccgt cttcgaggtc gcgagcacga cggccgtgag gacgtcgggc
     5161 gagccgccgg acgctccgtc ggaccacgac ggatccttcc aggtctcggg atcgaggccc
     5221 tcggccacga gattgcgggg ccttcgaaag cccgaccgcc ggtcctggcg ccggaggccc
     5281 cagcgcagtg cgtcctcggc ccagcgctgg aggcgcgggt cgagcgtcga atcgacgcgc
     5341 aggccctccc ggtagagctc gctctcgccg tactgctttt cgaggtactg gcggatctcc
     5401 tcgcagaagt aaggccccac cgtggactcc cgggtgccct tggagacctc gatcggggcc
     5461 gcggcggcgg ccgcacgctg gtcctccgag atgtatccct cctcgcgcat ccggcggagg
     5521 acgaagtcgc ggcgcgactt tgcggccccg gcgttgcgca cgggcgagta gtacgccggg
     5581 cgctggatga tcccggcgag gagcgcggcc tcgggcagcg tcaggacctt cgcggcgtgc
     5641 ccgaagtacc aacgcgatgc cgcctcgacg ccgtagttgc cgtgccccca cgggatctgg
     5701 ttgaggtaca tcgtgaggat ctggtccttc gagaactggc gctcgatctc gacggtcagg
     5761 aaggcctcgt tcaccttgcg ggcgatcgtc ttctcgggcg agaggaacac cgcgcgcgcg
     5821 agctgctggg tcaacgtcga ggcgccctgt gacaagcggc gcgtcgcgat gtccttcagg
     5881 agggcgccgc cgattcgtac gatgtcgatg ccgctgtggt cgtagaagcg cgcgtcctcg
     5941 gccgcgagga gcgccttcac catgacttcg gggatgtcct tcctctcgat gaggacgcgg
     6001 cgctcgatcg cgaaccgcgc gatcgtggag ccgtcggcgc cccggatctc cgtgacgatg
     6061 tcgggccggt acgtcgcgag ctcggagacc cgagggatgc ggatcgcgcg cgacagcccg
     6121 taccccgcgg cgacgccgag gccggcgacg aggaggacgg ggacgagcag gacggcgagg
     6181 gccgcaatgc ggcgtgcgct cacggcccat tctcgcacgc acggttcgcg tggtaccgtc
     6241 ccgcgtcgcg gaggaaccat ggccgaagaa cccgttccgc cggctccggc gccgctgatc
     6301 ccgaagctcc ccacgacgct gccgttcgag gacgaggcgg ggcgcgcgcg ccgcgacggc
     6361 tgcctgaagt ggggcctcgt cggctgcgcc ggggcgtccg tcgtcctgat cgtcgggatg
     6421 ctcttcgtcc tctcgaacgc gaagaagctc atgggtctcg ccttcgagaa gatcggcgac
     6481 caggtcgtcg cggccgcggg gcccgaggtc accccggcgg agaaggagcg tttccggacg
     6541 gcgctcaccg cgttcgccga gcgcgcgaag gcgggcacgg cgaagcccgc cgccgtgcag
     6601 acgtggcagt cgcaggtcac gggcgcgctc gcggacggcc gcgtgaccgg agacgagatg
     6661 cgctccctga ccgtctggct cgagacgtcc ggcgcggcgc cgcctccggc tccggcgaaa
     6721 tagtttcgtg gcggacttcc aggtcctctc cccgctcacg ccgcagggcg accagccgcg
     6781 cgcgatcgag cagctcgtcg cgggtttccg cgaggggcgg gagaagcagg tccttctcgg
     6841 cgtcacgggc tctggcaaga cgttcacgat ggcgaaggtc gtcgaggcgc tgaaccgtcc
     6901 gacgctcgtc ctctcgcaca acaagacgct cgcggcgcag ctctatcagg agttccggtc
     6961 cttcttcccg aagaaccggg tcgagtactt cgtctcgtac tacgactact accagcccga
     7021 ggcctacgtc ccgcagtccg acacgtacat cgagaaggaa acggacatca acgaggagat
     7081 cgacaagctc cggatcgcgg cgacggccgc gctcttcgag cggcgcgacg tgctcatcgt
     7141 ggcctccgtc tcgtgcatct acggcctcgg cagccccgag tcgttcggga agatggcgat
     7201 cgagatcgag aagggtgccc gttacgggat gacgtggtac ttgaagcgcc tcgtcgaggc
     7261 ccagtacgaa cgcacgaaca tggacctctc gcgcggcacg ttccgcgtgc gcggggacgt
     7321 gctcgacgtc gtgccgtcgt acgaggagct gggcctccgg gtcgagttct tcggcgacga
     7381 gatcgagcgg atcacgcgct tcgagaccgt gacgggccgg aacgtcgggg agatcccgcg
     7441 cttcgagctc ttcccgaagt cgcactacgt cacgcccgag gaggtccgaa agcgcgcgct
     7501 cgggtccatc gaggccgagc tcgtcgtgcg gctcgaggag ctgaaggagc agaacaagct
     7561 gctcgaagcc cagcgcctcg agcagcggac gcgcttcgac ctcgagatga tgcgcgagat
     7621 cggctactgc gccgggatcg agaactactc gcgccacctg tccggccgca agcccggcga
     7681 gccgccgccg acgctcctcg actacttccc gtccgactac ctcacgatcg tcgacgagag
     7741 ccaccagacg gtcccgcagg tgcgcgcgat gtaccacggc gaccagtccc ggaagaagac
     7801 gctcgtcgag tacggcttcc gccttccctc ggccctcgac aaccgcccgc tccagttcga
     7861 ggagtggctc gagaggacgg ggcagcgcct cttcgtctcg gcgacgcctg cgccgtacga
     7921 actccaggaa acgggcggcg aggtcgtcga gcaggtgctg cgcccgaccg gccttctcga
     7981 cccgccgatc ttcgtgaggc ccgtgaaggg tcaggtcgac gacctcctct cgagcgtgaa
     8041 ggagcggacc gcgaagaacg agcgcacgct cgtcacgacg ctcacgaagc gcatggccga
     8101 ggacctcacg aactacttcc tcgagctcgg gatccgcgtg cggtacctcc acagcgacgt
     8161 cgagacgctc gagcgcgtcc agatcgtggg cgacttccgc cgcggcgtct tcgacgtcct
     8221 cgtgggcgtg aacctcctgc gggagggcct ggacctgccg gaggtctccc tcgtcgccgt
     8281 cctcgacgcc gacaaggaag gcttcctcag gtccgagacg tcgctcatcc agacggcggg
     8341 tcgcgccgcg cgaaacgtga acgggatcgc cgtgttctac gccgacaaga tcacggattc
     8401 catgaagcgc gcgatgagcg agaccgagcg ccggcggaag ctccaaggcg actggaacgc
     8461 ggcacacggc atcgtgcccg agacgatcgt gaagtcgctc gactcgccgc ttctccagat
     8521 ggcggacctc gactacctcg agccgagcgg agtcgagcgc cgcccgaagg cgaaggacgc
     8581 gccctcgctc gaccgcgaga tcgcggacct cgagaaggag atgaaggtgg cggccaagga
     8641 gctcgagttc gagaaagcgg cgcagctccg cgaccgcatc cgcgaactga ggcagatgaa
     8701 gctgttctaa ggcccccggc cctgaaatgg cgggcgccgg cggccgcccg ggcctatctt
     8761 tccccgagaa aggagtgccc cgcgatgaaa cgcgtcctct ttctcgccgc gatcgtggcg
     8821 ctcgtcttct tcgcgtgccc ggcgcactcc gtgatcgcct gacgcggccg gcgcgagcgg
     8881 aaaaatggtg ggcccgacag ggatcgaacc tgcgacaagc ggtttaggaa accgctgctc
     8941 tatccaactg agctacgggc cctcggccgc attctagcgg cgtccgctcg cgagcgcgcg
     9001 ttcgaggttc gtccgcgcgt gctcgtagtc gggcctcagg cgcagcgcct cccggaagtg
     9061 ctcgattgcc tccgcctcct gcccctgccg catgagagcc gcgccgaggc cgttgtgcgc
     9121 gaggtggtcc ccgggggacc cgaggtgcag cgcttcgcgg tacgacgcga tcgcctcctc
     9181 gacacggccg gcgcgcgcgt ccgccttcgc gaggttgatc agggcggcgg tgttcgcggg
     9241 atcgacgcgg agcgccgagc ggagcagggc cgccgcgcgt tccgtgcggc cccgccgctc
     9301 ctcgatcagc gcgaggttga ggagggtggg gacctcgtcc ggcttgaggc ggtacgacgc
     9361 ctcgagatgc gcggcggcct cgggatcgcg gccgctctcc atgagccaga tcccgtagtt
     9421 cgtgtggcag atgaagcact ccgggttctt cacgagggtt gcgctccaga gcgcctcctc
     9481 gtcgcggtag atccggccct ggcggaaggt gagagccccg aggagggcga tcgcggccac
     9541 ggcaaacgcg gcggcggcgc gcgtcgcttt cgcgctccac ccggcggtcc acgcggcggc
     9601 tccgcatacg atcgatgccg cagcgaccgc gacggcctga taggcgaaat ggtccgcgac
     9661 ccacgagaag cgcatcgggt agacgttgaa gaaacccatc gcgggaaaga ggaccccgcc
     9721 gaagaggagc aggaaggcga acgggccgcg gccgacgcgc acccggaacc gccacgcgag
     9781 ggcgagaagg acgagcgcgc cgagcgccgg cagccactgg aggggggcgt gcgcgtcgac
     9841 cttccagcgc gggtagatga aagagaggtc gagaggaaga aggaatttct gcaagtagaa
     9901 gaacaccgtt cggccggcga gcacgatgcg gtcggcgaca ctcagcgtcc actccgagcc
     9961 gacggccatg acggaagtgc gctcgagcca cgccgtgtgg gcgccgaggg cgatcccggc
    10021 gccgaaccag ggaacgagcg gccgggcgtc ggccgccttc acgcgcccct cccgccacca
    10081 gacgaggacg aggagaactg ggggaaccac gcttgcggtc gtcttcgcga ggagagcgag
    10141 gacgaagaag aagaatccta tgaaagtgag agcgcgtgcg gacggacctg cgtcggaatg
    10201 gggcggcgac ccgcgtctct taccctttct aagagaatct cctgtcttct cttcgtcttc
    10261 cctcctatct cgcgactcga agtacgcgtg catcgcgagc agggagagga agagcgaaag
    10321 ggtgttcttg cgctcggtga cccacgcgac ggactccacg cacatcgggt ggagcgcgaa
    10381 cgccgcggcc gcgagccacg cgccgcgcag cccgaggcgc gcgaggagcc gccagagcag
    10441 gagggccgaa ccggcgtgca gcagcacgtt gacgaggtgg tacccgaacg ggtcgaggcc
    10501 ccagaggcgc ttctcgaccc agaacgacgt gaacaccatc gggtagtact gctcgttggc
    10561 ccgaggttgc gtccagatga ggcgaaggcc gtcgaggccg tccagcgtgc ggttcgcggt
    10621 gaggtacgtg tcgtcgttcc agatgaagcc ccccgcgagc gccggcagaa acgccgtcac
    10681 caccgccgcg aagaggagga gcccggggag ggcctttctc gccaccggcc ccacgataag
    10741 ccagcgcccc gcccgttgcg gtgtcaggga gagttagact tgacgggatg aagaaggacg
    10801 agcggcggac cggggaacgc gcgagggccg tgatcccgat cggcgtccgg atcggcgagc
    10861 gcaagctgac ggccggctgc acactggacg cgggccccac cggggcgctc atcctctgca
    10921 ccgagaacgt cccggtgggt accgagttgc acgtcacgaa tctccagtcg gacgagtggt
    10981 tcacgtg
//
LOCUS       DAOMFY010000120        10868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1877_length_10868_cov_8.182743, whole genome shotgun sequence.
ACCESSION   DAOMFY010000120 DAOMFY010000000
VERSION     DAOMFY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_10868_cov_8.182743"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLB01_10895"
     CDS             complement(<1..490)
                     /locus_tag="PLB01_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77853.1"
                     /translation="MSRERLRASLPYAALFVLSSALTAWWIASARIPSSADQAVVALM
                     ARHILEGRGHPVFYWGATYGGTLEPHLLAPLFGLFGATPEAFRGFYVALWAAFWGGTV
                     AFTARFFGRSAALVAAAYLAVPPFFLPYKVLTSDGAYASVALLGLAALWCACEADARL
                     EAG"
     gene            complement(532..987)
                     /locus_tag="PLB01_10900"
     CDS             complement(532..987)
                     /locus_tag="PLB01_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77854.1"
                     /translation="MSKVRIARALLVASAVAAGLSATSAQAQTGLQYFALTPCRAYDS
                     RPSNPLPAATQRNVTIKGVCGVPATAKAVSLNATIATPTIDGFFSMWPAGGTFPVVST
                     LNFLAGEPAIANGAIVPLGSSPTQDLALAYGTAGGGQTHAILDVTGYFQ"
     gene            complement(1059..2984)
                     /locus_tag="PLB01_10905"
     CDS             complement(1059..2984)
                     /locus_tag="PLB01_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQQ77855.1"
                     /translation="MRLAKTVGPAFLALTIGVAGETSAQSLPPGYAVRPGFPKVLPGS
                     TGHPSNEPVWANLGLPGGRSLILGTSSKELYVLNQDGSVAAGWPQPLGGQAVSSAAVG
                     DLDGDGIPDIVVGFGGGVWPPADPATVGGVAAFKRDGTLLWVVLSANDVPTHSSFPLG
                     VVSTPAIGDIDADGHADVVWGSWDGHIYAVDGRNGSAKAGTWPLFVRDTIWSSPSLYD
                     LDGNGKLEIIIGTDSHADATANPPGVPPTINGGRLHVLTYLGQEFPGFPFDVDQIIMG
                     SPVVGDITGDGSPKIVFGTGTYWGNPAPCGSGIGTLRKRAIYALRCNGQLLPGFGVAN
                     MTSGEVTTSPALADLDGDGVLDVVVTDMDCSTGAAQNFNVYAFKGTGALMWKTKPLAY
                     AGVNLSAGQPVVADVYGDSKLEVLVPTNSEICILSNTGVQLTDAGAPHSAGEFSLYMP
                     SSSSGATLDVVGGVLNVAAASSTSGNVSIHAWTTGKSAPPVWGSYRRDSGRLAVAPNA
                     GTCAPRAPAPTVFHPLPPCRVLDTRGTFGPLGGPALAPMTPRSFNVTGVCGIPALAVA
                     ISANLTVTNVTGSGELIVFPSDVAQPGTSSVSFRAGRTRANNALVYLSATTTTFSVFN
                     NCIAGVDFILDVNGYFQ"
     gene            complement(2981..4381)
                     /locus_tag="PLB01_10910"
     CDS             complement(2981..4381)
                     /locus_tag="PLB01_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77856.1"
                     /translation="MRSGVRAAAFAPLAVLLASTPLSAQPVQGGPLPGPLPLFPADNW
                     WNVDVSAAPVDPASAAFIAWIGAAKGMHPDFGGDVSTGSTAIYGMPYVVVPGTQALEP
                     MAFDYASESDAGAPGRPAGYPIPVEAKTQSHWIEGGYPGNCDASYAGSPCRGDKHMLI
                     VDADHRLLFETWNTRCLPSGSPACAWRAGSGAVFSLDSNARRPDGWTSADAAGLAILP
                     GLVRWDEAYGASPIRHAFRVTVRDTNGYVYPASHVAGSNANAPPMGARLRLRASKDIS
                     GFTAPVQRIFQAMKTYGLIVADNGSDMYVQGTYDVRWDNGVLNPAFGAITAGDFEVVQ
                     LGWQPSAPPAATRFRSVPPCRVVDTRTAQGAPPIAANASRTFVLTGTCGVSATAVTVS
                     ANVTVVAPRASGDLVFFPAGTGVPNASTLSFRAGRTRANNTQILLSSDGTGRATAKNN
                     AAGALDLVIDVSGYYE"
     gene            complement(4378..6156)
                     /locus_tag="PLB01_10915"
     CDS             complement(4378..6156)
                     /locus_tag="PLB01_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQQ77857.1"
                     /translation="MALHFRCTARRVAARALALLTALVIGGPLAAARPDGPLSAGASG
                     KIVDLSGGRGEGELLRFDAGLVPALLALAPEEGVAVLDWPFAPGVRRAVRVERHEVWA
                     DGAKVFRVDAGGRTEIPRSRLVFFWGSDTEGAGGRVLVAVDPSTGALTALAHGPEGVH
                     EIARDPSDAAGRHLLAAPARPDFTGVRCGQEDFPDTASLPASVARHSAAGRSAAVLGS
                     LYKGILAIDTDTEYLASFGNNTTTTTNYIATLVANINVMYERDLNLRLVVGTTFLRTG
                     TDPWTVTGSGASGSQLNEFTSYWNTNYPKATYPRSFAVLFSGKSTSGLSGIAWVAGSV
                     CGSGNDYSFNQISANGSWLWGDTLIVGHEVGHNMGSPHTHCYANPAPDRCYAGESCYS
                     GATSCPAPQTINGVANVTGTIMSYCHLSGISGCGTSLVFHPLTVSRYVGPALDAGASS
                     ACISLVVPPPPPPPPVSAATGFNAVPPCRLLDTRNAAGPLGAPSLGALASRSFVATGN
                     CGIPSGAVAISANVTVVSPTVAGDLVAYPNGLASPPGTSTISFRAGRTRANNALVYLA
                     GDGSFLVTNRAAGSLDLVVDVNGYTK"
     gene            complement(6380..6904)
                     /gene="smpB"
                     /locus_tag="PLB01_10920"
     CDS             complement(6380..6904)
                     /gene="smpB"
                     /locus_tag="PLB01_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQQ77858.1"
                     /translation="MKSRLDREERKKDARAAAKNSGVRPIATNRRAFHEYFVEEKFEA
                     GLALTGSEVKSLREGKASLQDGYVAVEGGEAWLWNVHVPPYGAASYNNVDAKRKRKLL
                     LHKREIVRLASKIQKSGATCVPLTLYFKGARVKVEIALVTGKKLHDKRETIRRREADR
                     EARSAMKDARAPKR"
     gene            complement(6915..8600)
                     /locus_tag="PLB01_10925"
     CDS             complement(6915..8600)
                     /locus_tag="PLB01_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQQ77859.1"
                     /translation="MVAQKWRPAKAAFYTLDPGGAVFRLKAQFGFTRGDRLAERMGRN
                     EPLVNQIYEHREPTFINNVNQAGRLADTMIQSTSTRMLVAPLYLDGRIMGLVDMRDKA
                     GRVPFTHDDALEMADLLRRFAVELRRLSDAKSAVSILREPPPVVERPLDPPTPSPAMP
                     SPLEKTSGFAAIPATSGIFRRAPMPPSVSEPVDTALPGLAVRTLRLVEETLARGGAQR
                     LAAAPAGGLSPREAEFVQLYLPTCLNYDEVEVAAVSSFQPSVGIVTVTSRRPLDADVV
                     PALTENLEKIFAKTAVPFAFTRDPVVRPLDAPGEGRPVRRADIAAIQSSVLDSSPEGV
                     VLFSLLYRHGPNPESREALRSAHVVLRNVLAGTRGEARFRESYRGLVNKLLEPGLIRR
                     TALKTHSFNVGRMARKLAVQIGVAPMDVEQITVAAILHDVGLRELNYDTLYAKRTLTD
                     EELTLLKTHPRVGAYLVEDIPWPYPIAPLIRHHHERWDGGGYPDGLRGEQIPLGSRII
                     HLCEAFDAMTAPTSYRAVLSDYQALDIIESKAGTQFDPELAPEFKRMVESPSD"
     gene            8857..9738
                     /gene="lpxC"
                     /locus_tag="PLB01_10930"
     CDS             8857..9738
                     /gene="lpxC"
                     /locus_tag="PLB01_10930"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HQQ77860.1"
                     /translation="MLVQTTLARPVRISGIGLHSGRHVHVVLRPAGANHGIVFWRTDV
                     DAFVPAIAEAAGRFDHATSLGERGRDVSTVEHLLSAAVGAGLDNLLVEVDGPEVPILD
                     GSALPWLEAFSKAGAAPLAALKAPFTPSRVLSLTASGGKKLEIRPARDLRVTYTIDFP
                     NPAVGRQSLTVVLSPDTYATHVAPARTFGFLSEYELLKQHGLARGASPENCIVVGEDR
                     VENGDLRFPDEFVRHKVLDLLGDLALVGRPVTGHVVAHKAGHAMHASLAALLREEVRA
                     ARHAPSQAPAAARHVNR"
     gene            9748..10326
                     /locus_tag="PLB01_10935"
     CDS             9748..10326
                     /locus_tag="PLB01_10935"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HQQ77861.1"
                     /translation="MSTRPGPTAPLLLGALLALAGVSSAQAPPAAEVPGVSSWLTGRE
                     LHVEAKLSPGLPEAARQRLASGLPTTVAWTIRLFVFRNLWWDALKDERLYAVTATYRP
                     DTSDYLVERRLDARLLDTQVVPTRDEAAASLGRVPGLPVFTMGDHLLGKKLVVRLRCR
                     YGSGVALGVVPTSDETDWARSGIFEWTAAGAR"
     gene            10302..>10868
                     /locus_tag="PLB01_10940"
     CDS             10302..>10868
                     /locus_tag="PLB01_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77862.1"
                     /translation="MDGGGRPLTESVPPVPPYRWTRDNRILLAIGLALGAALAGGYAL
                     VLRTRDLTAAAATNRVLLFVLFYIVVVLILVLLFVLVRNGIKLVLESRRGVFGSRFRA
                     RVVAAYVGLALLPISLLILPTTGLLQKSVERWFAPPVENTIRAGRQVADLVRARSAAL
                     ERRTADRLLPRLAAAPERDVVALLSSARE"
BASE COUNT         1598 a         3811 c         3892 g         1567 t
ORIGIN      
        1 ggcccgcctc gagccgcgcg tcggcctcgc aggcgcacca cagcgccgcg agcccgagca
       61 gggccaccga cgcgtacgcg ccgtcggacg tcaggacctt gtacgggaga aagaagggcg
      121 ggaccgcgag gtacgccgcg gccacgagag cggcgctgcg gccgaagaat cgcgcggtga
      181 aggcgaccgt gccgccccag aacgccgccc acagcgcgac gtagaacccg cggaaggcct
      241 cgggagtcgc gccgaagagc ccgaagagcg gcgcgaggag atgcggctcg agcgtcccgc
      301 cgtacgtcgc gccccagtag aagaccgggt gtccgcggcc ctcgaggatg tgccgcgcca
      361 tgagcgcgac gacggcctgg tccgcgctgg acgggatgcg cgccgaggcg atccaccacg
      421 ccgtgagggc gcttgagaga acgaaaagag cggcgtaagg cagggacgcc cgaagccgct
      481 ctcgtgacac ctctggaatc taacccggag gccccggcct ccgggacctg cttactggaa
      541 gtagcccgtg acgtcgagga tggcgtgggt ctggcccccg cccgccgtgc cgtaggccag
      601 ggcgagatcc tgtgtcgggc tcgacccgag cggaacgatc gccccgttcg cgatggccgg
      661 ctcacccgcg aggaagttca gcgtggacac gaccggaaag gtaccgcccg cgggccacat
      721 gctgaagaag ccgtcgatgg tgggcgtggc gatcgtcgcg ttgagcgaca cggccttcgc
      781 cgtcgccggc acgccgcaga cgcccttgat cgtcacattg cgctgggtgg cggccgggag
      841 agggttggag gggcgcgagt cgtaggcgcg gcagggcgtg agcgcgaagt actgcaggcc
      901 ggtctgggcc tgcgccgacg tggccgagag gcctgcggcg acggcggacg cgacgagaag
      961 ggcgcgggcg atacgaacct tgctcatgga ctttctcctc tttccggctt tgaacggagt
     1021 ctaactgaga ttgaatttcg attggtagtg tcggcgcctc actggaagta cccgtttacg
     1081 tccaggatga agtccactcc cgcgatgcaa ttgttgaaaa cactgaaggt tgtggtcgtc
     1141 gccgagaggt agacgagggc gttgtttgcc cgggttctgc ccgcccggaa gctcacggag
     1201 ctggtgccgg gttgcgcgac atccgacggg aacacgatca gctcccccga tccggtcacg
     1261 ttcgtcacgg tcaggttcgc ggaaatggcg accgcgagcg cggggatccc gcacacgccc
     1321 gtcacgttga agcttcgcgg cgtcatcggg gcgagcgccg gcccgccgag cggaccgaac
     1381 gtcccgcgcg tgtccagaac gcggcagggc ggcaggggat ggaacacggt cggggcggga
     1441 gcgcgcgggg cgcacgtgcc cgcattcggc gcgacggcga gccgcccgga gtccctccgg
     1501 tacgagcccc agacgggggg ggcggacttg cccgtcgtcc acgcgtgaat gctgacgttt
     1561 ccggaggtcg aggacgcggc cgcgacgttc aggacgcctc cgacgacgtc gagcgtggcg
     1621 ccgctcgagg aagagggcat gtagagagag aactctccgg cgctgtgcgg cgcgccggca
     1681 tccgtcagct ggacacccgt gttcgagagg atgcagatct cggaattcgt ggggacgagg
     1741 acctcgagct tcgagtcgcc gtagacgtcg gcgacgacgg gctgccccgc gctcaggttc
     1801 acgcccgcgt acgccaacgg cttcgtcttc cacatcagcg cgcccgtgcc cttgaacgcg
     1861 tagacgttga agttctgcgc ggcgcccgtc gagcagtcca tgtccgtgac gacgacatcg
     1921 aggacgccgt ccccgtcgag gtcggcgaga gcgggcgagg tcgtgacctc gcccgacgtc
     1981 atgttcgcga cgccgaaacc cggaaggagc tgcccgttgc accgcaacgc gtagatcgcg
     2041 cgcttgcgaa gggtgccgat cccgctcccg cagggcgccg ggtttcccca gtacgtgccg
     2101 gtcccgaaga cgatcttcgg gctgccgtcg cccgtgatgt cgccgacgac cggcgagccc
     2161 atgatgatct ggtcgacgtc gaacggaaac cccgggaact cctgcccgag gtacgtgagg
     2221 acgtgcaggc ggccgccgtt gatcgtcggc ggaacgccgg gcggattcgc ggttgcatcg
     2281 gcgtgcgagt ccgtgccgat gatgatctcg agcttgccgt tcccgtcgag gtcgtagagc
     2341 gacggagagg accagatcgt gtcgcgcacg aacaggggcc aggtcccggc cttcgcgctg
     2401 ccgtttcgcc cgtcgaccgc gtagatgtgt ccgtcccacg atccccagac gacgtccgcg
     2461 tggccgtcgg cgtcgatgtc gccgatcgcc ggagtcgaga cgacgccgag cggaaaactg
     2521 ctgtgagtgg ggacgtcgtt cgcgctcagc acgacccaga gaagcgttcc atccctcttg
     2581 aacgcggcga cgccgccgac cgtggcggga tccgcgggag gccaaacgcc tccgccgaac
     2641 ccgacgacga tgtccgggat tccgtccccg tcgaggtcgc cgaccgccgc gctgctcacg
     2701 gcctgccccc ccagcggctg cggccagccc gcggcgacgc tgccgtcctg gttcaggaca
     2761 tagagctcct tcgagctcgt gccgaggatg agggacctgc cgcccggaag gccgaggttc
     2821 gcccacacgg gctcgttcga ggggtgcccg gtgctgccgg gcagcacctt cggaaacccg
     2881 ggccgcacgg cgtagcccgg gggcagggac tgggcggagg tctcaccagc gacgccaatc
     2941 gtcagcgcga gaaaagccgg accgacagtt ttcgcgagcc tcattcgtag tacccgctga
     3001 cgtcgatcac gagatcgagc gcgccagccg cgttgttctt cgccgttgca cgccccgtgc
     3061 cgtcggacga caggagaatc tgggtgttgt tcgcacgggt ccgtcccgcc cggaagctta
     3121 acgtcgaggc gttcggcacg cccgtcccgg ccgggaagaa gacgaggtcg cccgaggccc
     3181 ggggggcgac gaccgtcacg ttggccgaca cggtgactgc cgtcgcggag acgccgcagg
     3241 tgcccgtgag aacgaacgtg cgcgaggcgt tcgccgcgat cgggggcgcg ccctgcgccg
     3301 tccgcgtgtc cacgacgcgg cacgggggaa ccgagcgaaa ccgcgtcgcg gccggaggcg
     3361 ccgacggctg ccagcccagc tggacgacct cgaagtcccc ggcggtgatc gcgccgaacg
     3421 cggggttcag gacgccgttg tcccagcgga cgtcgtacgt gccctgcacg tacatgtccg
     3481 acccgttgtc cgcgacgatc aggccgtacg tcttcatggc ctggaagatc cgctgcaccg
     3541 gagcggtgaa tcccgagatg tccttcgacg ccctgagccg caggcgagcg cccatcggcg
     3601 gcgcgttcgc gttcgaaccc gcgacgtgcg aggccgggta gacgtacccg ttcgtgtcgc
     3661 ggaccgtgac gcggaacgcg tggcggatgg gcgaggcgcc gtacgcctcg tcccagcgca
     3721 cgaggccggg aaggatcgcg agaccggccg cgtccgcgct cgtccagccg tccggccgcc
     3781 gcgcgttcga gtcgagggag aagaccgcgc ccgagcccgc gcgccacgcg cacgccggcg
     3841 agcccgaagg caggcagcgc gtgttccacg tctcgaagag gagccggtgg tccgcgtcca
     3901 cgatcagcat gtgcttgtcg ccgcggcacg gggagcccgc gtacgacgcg tcgcagttgc
     3961 ccgggtagcc gccttcgatc cagtggctct gggtcttcgc ctcgacgggg atcggataac
     4021 ccgccgggcg gcccggcgcg ccggcgtcgc tctccgaggc gtagtcgaac gccatcggct
     4081 cgagcgcctg cgtcccgggc acgacgacgt acggcatgcc gtagatcgcg gtcgagcccg
     4141 tcgagacgtc gccgccgaag tcggggtgca tgcccttcgc agcgccgatc caggcgatga
     4201 acgcggcgct cgccgggtcg acgggcgcgg cgctcacgtc gacgttccac cagttgtcgg
     4261 ccgggaagag cggcagcggc cccgggagcg gcccgccctg caccggttga gccgacaggg
     4321 gcgtgctcgc gagcaggacg gcgagggggg cgaaggcggc cgcgcgaacc ccggacctca
     4381 cttcgtgtaa ccgttgacgt cgacgacgag gtccagcgag ccggcggccc tgttcgtgac
     4441 gaggaagctg ccgtcccccg cgagatagac gagcgcgttg ttcgcgcgcg tgcggccggc
     4501 gcggaagctg atcgtcgagg ttcctggagg cgacgcgaga ccgttcgggt acgcgacgag
     4561 gtcgccggcg accgtcgggc tcacgaccgt gacgttcgcg gagatcgcca cggcgcccga
     4621 cggaatgccg cagtttccgg tcgcgacgaa cgaccgtgac gcgagcgccc cgagcgacgg
     4681 agcgccgagc ggacccgccg cgttgcgcgt atcgagcagg cggcacggcg ggacggcgtt
     4741 gaagcccgtc gccgcggaca cgggaggcgg gggcgggggc ggagggacca cgagcgagat
     4801 gcacgcggac gaggcgccgg cgtcgagcgc cgggcccacg taccggctca ccgtgagcgg
     4861 gtggaagacg agcgaggtgc cgcagcccga gattccggac aggtggcagt agctcatgat
     4921 cgtgccggtg acgttcgcaa cgccgttgat cgtctggggc gccgggcagg acgtcgcacc
     4981 cgaatagcag ctctcgcccg cgtagcaccg gtcgggcgcg gggttcgcat agcagtgcgt
     5041 gtgcggcgat cccatgttgt ggccgacctc gtggccgacg atcagcgtgt cgccccagag
     5101 ccagctcccg ttcgcggaga tctggttgaa gctgtagtcg ttcccgctgc cgcaaaccga
     5161 gcccgcgacc cacgcgatgc ccgagagccc ggacgtgctc ttgcccgaga agaggacggc
     5221 gaacgacctc ggatacgtcg ccttcgggta gttcgtgttc cagtaacttg tgaactcgtt
     5281 caactggctg ccgctcgcgc ccgagcccgt gaccgtccac gggtccgtgc ccgtccggag
     5341 aaacgtcgtt ccgacgacga gcctcaggtt gaggtccctc tcgtacatca cgttgatgtt
     5401 cgcgacgagc gtcgcgatgt agttcgtcgt cgtcgtcgtg ttgttcccga acgaggcgag
     5461 gtactccgtg tccgtgtcga tcgcgaggat tcccttgtag agcgacccga gcaccgccgc
     5521 gctccggccc gcggccgaat gccgggcgac ggacgccggc agcgaggccg tgtccggaaa
     5581 gtcctcctgg ccgcatctca cgccggtgaa gtccggacgt gcgggcgctg cgaggaggtg
     5641 gcggcccgcg gcgtccgaag gatcgcgcgc gatctcgtgt acgccctcgg gcccgtgcgc
     5701 gagcgccgtc agcgcgcccg tcgacgggtc cacggcgacg aggacgcgcc cgcccgcgcc
     5761 ctcggtgtcg ctgccccaga agaacacgag ccgcgagcgc gggatctcgg tccggcctcc
     5821 cgcgtcgacg cggaagacct tcgcgccgtc ggcccagacc tcgtgccttt cgacgcgcac
     5881 ggcgcggcgc acgcccgggg cgaacggcca gtcgaggacg gcgacgccct cctcgggcgc
     5941 gagcgcgagg agcgccggga cgagccctgc gtcgaaacgc agaagctcgc cttctccccg
     6001 cccgccggaa agatccacga tcttcccgct ggcgccggcg gacagaggcc cgtcgggacg
     6061 agcggccgcg agcggcccgc cgatcacaag cgccgtcaga agcgcgagag cccgcgcggc
     6121 gacacgtcga gccgtacacc ggaaatggag cgccatgtgt cccctcgctg caccgtccat
     6181 tgcggaaccc gtgccgaccc ggagccttcg ccggggagtg cgccgggccc acggaaaatc
     6241 acgcctctta cgtgacttgg gtcaccggac gaggggaaaa ggcggatgcg gggccgcgaa
     6301 cgaacgcccg gcatccgtca aaatttgttc gccgcgccgc gacggtccct cgaaatttga
     6361 cccgggcgcg gacccggcgt cagcgcttcg gcgcccgcgc gtccttcatc gcggaacgcg
     6421 cctcgcggtc ggcctcgcgg cggcggatcg tctcgcgctt gtcgtgcagc ttcttgcccg
     6481 tgacgagcgc gatctcgacc ttcacgcgcg cccccttgaa gtacagagtc agcgggacgc
     6541 acgtggcgcc cgatttctga atcttcgagg cgagccggac gatctcgcgc ttgtggagga
     6601 gaagcttgcg cttcctcttc gcgtcgacgt tgttgtagct cgccgcgccg tacggcggca
     6661 cgtggacgtt ccagagccag gcctcgccgc cctcgacggc gacgtagccg tcctggaggc
     6721 tcgccttgcc ctcgcgcagc gacttgacct cgctgcccgt gagcgcgagg ccggcctcga
     6781 acttctcctc gacgaagtac tcgtggaacg cgcgccggtt cgtcgcgatc gggcggaccc
     6841 ccgagttctt cgcggcggcg cgcgcgtcct tcttccgctc ctcgcggtcg agccgcgact
     6901 tcacgcgcgc cccgtcagtc cgagggactc tcgaccatgc gcttgaactc gggcgcgagc
     6961 tcggggtcga actgcgtgcc cgccttcgat tcgatgatgt cgagcgcctg gtagtccgag
     7021 agcaccgccc ggtaggacgt gggcgccgtc atcgcgtcga aggcttcgca caggtggatg
     7081 atgcgcgagc cgagcggaat ctgctcgccg cgcaggccgt ccgggtagcc gccgccgtcc
     7141 cagcgctcgt ggtggtgccg gatgagcggg gcgatcgggt acggccacgg gatgtcctcg
     7201 acgaggtagg cgccgacgcg cgggtgggtc ttcaggagcg tgagctcctc gtccgtcagc
     7261 gtgcgcttcg cgtagagcgt gtcgtagttc agctcccgca gcccgacgtc gtggaggatc
     7321 gcggccacgg tgatctgctc gacgtccatg ggcgcgacgc cgatctggac ggcaagcttg
     7381 cgcgccatgc gcccgacgtt gaacgagtgc gtcttgagcg ccgtgcgcct gatcagcccg
     7441 ggctcgagga gcttgttgac aaggccgcgg taggactcgc ggaaccgggc ctccccgcgc
     7501 gtccccgcga ggacgttgcg cagcacgacg tgcgccgagc ggagcgcctc gcgcgactcg
     7561 gggttcggcc cgtgccggta gaggagcgag aagagaacga cgccctccgg cgagctgtcg
     7621 aggacggagg actggatcgc cgcgatgtcc gcgcggcgca cgggccttcc ctcgccgggc
     7681 gcgtccagcg gccggaccac cgggtcgcgc gtgaaggcga acggcaccgc cgtcttcgcg
     7741 aagatcttct cgaggttctc cgtcagcgcc gggacgacgt cggcatccag cgggcggcgc
     7801 gacgtgacgg tcacgatccc gaccgagggc tggaacgacg acacggccgc gacttcgacc
     7861 tcgtcgtagt tcaggcaggt cggaaggtag agctggacga attcggcctc gcgcggcgag
     7921 aggcccccgg ccggagccgc cgcgagacgc tgggcgccgc cgcgcgcgag cgtctcttcg
     7981 acgaggcgca gcgtgcgcac ggccaagccg ggcagggcgg tgtccaccgg ctcggaaacg
     8041 gagggcggca tcggcgcgcg gcgaaagatg cccgacgtcg cgggaatggc ggcgaatccg
     8101 gacgtcttct cgaggggcga cggcatggcg ggggaaggcg tcggcgggtc gagcggccgc
     8161 tcgaccaccg gaggcggctc acggaggatg gacaccgcgg acttcgcgtc gctgagccgg
     8221 cggagttcga cggcgaaccg ccggaggagg tccgccatct cgagggcgtc gtcgtgcgtg
     8281 aaggggacgc ggcccgcctt gtcgcgcatg tcgacgagac ccatgattcg cccgtcgagg
     8341 tagaggggcg ccacgagcat gcgcgtcgac gtcgactgga tcatggtgtc cgcgaggcgg
     8401 cccgcctggt tcacgttgtt gatgaaggtg ggctcgcggt gctcgtagat ctggttgacg
     8461 agcggctcgt tgcgccccat gcgctccgcg agccggtcgc cgcgcgtgaa cccgaactgc
     8521 gccttcaggc ggaacaccgc cccgcccggg tcgagcgtgt agaaggcggc cttcgccggg
     8581 cgccacttct gggcgaccac ggcgagaaag tcctgataaa acttttcttc ctcgggcgcg
     8641 agcacacgtt cctccgggag tgtgccgagg atagcagccg cgtcccggac gcccggcggc
     8701 cggcggccgg cgggagtcct ccgaacctgt ggcgattccg ccgcaggcgc cgcggggatg
     8761 ccgcactccg ggggcgcccg tctggcggcg gaggcgcgca actcattgca aacactgggg
     8821 gacggccgtg gcacccacca tgcaaaacgg gccgcaatgc tcgtgcagac cacgctcgcc
     8881 aggccggtcc ggatctcggg aatcggcctc cacagcggcc gccacgtcca cgtcgtcctt
     8941 cgccccgcgg gggcgaacca cgggatcgtg ttttggcgca ccgacgtgga cgcgttcgtc
     9001 cccgcaatcg cggaagctgc gggccgcttc gaccacgcga cgtcgctcgg cgagcgcggc
     9061 cgggacgtga gcaccgtcga gcacctcctc tcggccgccg tcggcgccgg gctcgacaac
     9121 ctgctcgtcg aggtcgacgg acccgaggtc ccgatcctcg acgggagcgc gctcccgtgg
     9181 ctcgaggcct tctcgaaagc cggtgcggcg cccctcgcgg cgctcaaggc gccgttcacg
     9241 ccgtcccgcg tcctctcgct gacggcctcc ggcggcaaga agctcgagat acggcccgcg
     9301 cgcgacctgc gtgtgaccta cacgatcgat ttcccgaatc cggccgtcgg gcgccagtcg
     9361 ctcacggtcg ttctctcccc cgatacgtac gcgacgcacg tcgcgcccgc acgcacgttc
     9421 ggattccttt ccgagtacga gctcctgaag cagcacggcc tcgcccgcgg cgcctcgccc
     9481 gagaactgca tcgtcgtcgg agaggaccgc gtcgagaacg gcgacctgcg cttcccggac
     9541 gagttcgtcc gccacaaggt gctcgacctg ctcggcgatc tcgcgctcgt gggccgcccc
     9601 gtcaccggcc atgtcgtcgc tcacaaggcc gggcacgcga tgcacgccag cctcgcggct
     9661 cttctccgcg aggaagtccg cgcggcacgg cacgcgccct cgcaggctcc cgccgcagcc
     9721 cgccacgtaa accgttaaag tcgcggggtg tcgacgcgcc ccgggccgac ggcccccctg
     9781 ctcttgggag ctctgctcgc cctcgccggg gtgtcgtccg cccaggctcc tccggccgcc
     9841 gaggtcccgg gcgtctccag ctggcttacg ggccgcgaac tccacgtcga ggcgaagctc
     9901 tcccccggcc ttcccgaagc ggcgcgccag cgcctcgcgt ccgggctccc gacgaccgtg
     9961 gcgtggacga tccgcctctt cgtcttccgt aacctctggt gggacgcgct gaaggacgag
    10021 cgcctctacg ccgtgacggc gacgtaccgg cccgacacct ccgactacct cgtcgagcgg
    10081 cgactcgacg cgcgcctcct cgacacgcag gtcgtcccga cacgggacga ggccgccgcg
    10141 tctctggggc gcgttccggg cctgcccgtc ttcacgatgg gagaccacct cctcggcaag
    10201 aagctcgtcg tgcgcctgcg ctgccgctac ggcagcggcg tcgcgctcgg cgtcgtgccg
    10261 acgagcgacg agaccgactg ggcgcggtcg gggatcttcg aatggacggc ggcgggcgcc
    10321 cgctgacgga gtccgtgccg cccgttccgc cgtaccggtg gacgcgcgac aaccggattc
    10381 tcctcgcgat cggcctcgcg ctcggagccg ccctcgcggg cgggtacgcg ctcgtcctca
    10441 ggacgcgcga cctcacggcc gccgccgcaa cgaaccgcgt cctgctcttc gtcctcttct
    10501 acatcgtcgt cgtcctcatc ctcgtcctcc tcttcgtcct cgtgcgcaac ggcatcaagc
    10561 tcgtcctcga gtcgaggcgc ggcgtcttcg ggtcccgttt ccgcgcgcgc gtcgtggcgg
    10621 cgtacgtcgg gctcgccctc ctcccgatct cgctcctgat cctcccgacg acgggcctcc
    10681 tgcagaagag cgtcgaacgc tggttcgcgc cgcccgtcga gaacacgatc cgcgcggggc
    10741 ggcaagtcgc ggacctcgtc cgcgcgcgct cggccgccct cgagcgccgc acggccgacc
    10801 ggcttctccc gcgcctcgcg gccgcgcccg agagggacgt cgtcgcgctc ctgtcatccg
    10861 cccgcgag
//
LOCUS       DAOMFY010000121        10599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1921_length_10599_cov_8.326536, whole genome shotgun sequence.
ACCESSION   DAOMFY010000121 DAOMFY010000000
VERSION     DAOMFY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10599
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_10599_cov_8.326536"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLB01_10945"
     CDS             <1..360
                     /locus_tag="PLB01_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77863.1"
                     /translation="WDVESDATQDVLDRYHVLEPFAGLLPVDDQVRLAERFDFDALRW
                     GRVEAIFLIVMFGPLALSSVLGALAVFEAADVPRIAIFGGITLEQVLRLRRLAAGRLA
                     PSVLGVLVRPFARKLLA"
     gene            complement(362..1078)
                     /locus_tag="PLB01_10950"
     CDS             complement(362..1078)
                     /locus_tag="PLB01_10950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ77864.1"
                     /translation="MRRLDYGTDAPGVVRNLFVIGGLALLGGALLPWPLAGVNARGML
                     VSVGVTCCLAGALMILYAEWGKFRHRERMLAMVAWTGAENVLDVGTGRGLLALGAAKR
                     LTTGTAVGIDVWSPKDLTGNTADAFLANAEAEGVAERVEVRHADARALPFPDGSFDVV
                     LSNVCLHNIPDAGGRADACHEIARVLKKGGVALVSDFIRTADYARSFTAAGLAVTQTG
                     PHVLDVFPPLRVVRAEKRAG"
     gene            complement(1108..1899)
                     /locus_tag="PLB01_10955"
     CDS             complement(1108..1899)
                     /locus_tag="PLB01_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77865.1"
                     /translation="MRYAARPSLVPLSALVFLAAAGPTAATVDFFGSTTTCAGAGFNK
                     GETYPLFRGRTVILRVSGFAVDTASEVELSSLPGVHARFVQRVHGLGSSIDVELKTDA
                     DAALGDGEVRLHYPVEVSGFDRVKARVLDPPQIDSFAVEPAPGVTPSAQAQGSSPAFE
                     LVPGNLYTLVLKGKTLLDVRPKTPFAGQGRFTDVSVLSASDAELKVRLKPVLLGAATI
                     DRQSFTFEKLRCVEPPSGAASIRLTIAVPPTPAPAPKGPLPLPKK"
     gene            complement(1908..3251)
                     /locus_tag="PLB01_10960"
     CDS             complement(1908..3251)
                     /locus_tag="PLB01_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77866.1"
                     /translation="MKHLRSLALVSALAVLPAAGQVPIQKPPVKAATPRPTLPTEALQ
                     SLLPPPPDVTDWQPRGRAVAGGKIVITGTGFRPADFEAAVGPSKYRLPVRVSSSTSTR
                     IELDVPADVLGVSGIFVVAHRGTQAKTLETNYKLDQPTPALAELSVNGTAYPFMNKAV
                     RVKVREFPGARLDANAVTFGGTCSFHKQPGISFGTSNRTEDLSLDFAVVGWFERPGTC
                     RLEIRFHPLAADGSSMTRVTLEGNLTVATPVTYTFSSTAQLKSVLKPARVHSGLGSIC
                     EGTLPNGARVGIDDDGSDFSVVIRGGPLDVSCGYQTKEWFLPEGVTLTEIQWDSKHVG
                     NRCGLEGTMTGGDLVKYSFGRGVTTIRPEAGLPVSDHMAFGDREIVVDGVSFKSNLLG
                     PVTMIRPFYLGMQCVSMATVLTDSSGTHGPTIDPQAYRITLKKLVLEGPPNLKLP"
     gene            complement(3268..4569)
                     /locus_tag="PLB01_10965"
     CDS             complement(3268..4569)
                     /locus_tag="PLB01_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77867.1"
                     /translation="MRTRDAIARAALALLLTAGAASAQIPTPKIPTPAAPKVPPLPTA
                     TPTPRPAIVGPADRPPQITMRGEPTVSNWSPKEYVVQGDKIVFTGDLVSSEFHARLGQ
                     VDLPIIAHGNCATASCDTFIHVRVPDTFSSPGAPLVVWYGVNGPKKTLTPSFKVLKKP
                     RVASFKITDGPHINLSFTRGKDTKIEVELEDFDATAETFFRPSLWSPGCRAGGLAPKS
                     EPGGSPYRLKYDFSFFADASGKNCPMEIQPYGSRGPALSLPNVLVPSLETYTVSDTWD
                     LLEFTTPSGKKLSATASKGGLPCQLLSVGTAGTFSTGVVKEGGDLTFQLRNGALDEAC
                     RFETSPALLVRDDWAVKSVDWDFTEDSLCAAVNKFATQVSSGSTVTFFNDESAVFAVK
                     FEALCKPNGNDLPKNSHVFKARLSSVKVIGPAGKSWRDAFK"
     gene            complement(4610..5464)
                     /locus_tag="PLB01_10970"
     CDS             complement(4610..5464)
                     /locus_tag="PLB01_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77868.1"
                     /translation="MTRRSRPALLLGIALTAGSAGASIDQVETCTVWADKAPLLTRAA
                     HNDVTVTGFGVDLATSVTTTLPGTPVTVVSRKNGLGSNIVLRFAPPAVGNAAEGTVTL
                     HYFGGGTDVFNASLQAGPSVTSISFASEGGVSKSGGATRVTSLDPHVVVLKGTNLQEV
                     RIDDRAFFRSSLSNAKIVLQLPDELRVSFTSTAGDRDVGSRHFIAPGFCFNLMPEFSI
                     AFTVFDPPRTPTPTPTATQTRTPAPSPTPTKPPFGPIAPPTGVRPALPTPTPTPRATP
                     LSPIAPRK"
     gene            complement(5479..6627)
                     /locus_tag="PLB01_10975"
     CDS             complement(5479..6627)
                     /locus_tag="PLB01_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77869.1"
                     /translation="MRNSARRPAFAVSALLLSAAAGMPAAGTVQSIKYSYPGTCRAML
                     TAGPTDPVTLIFGEPLDLEIAGSGLEGATGGGSSPKQAAPGPRVVTPDGVEGAVLESS
                     AKAMKIRLLYRRGDPPRRSSPPAIVRIVQGSATLGELFVRLAARPQIDRIRLAIPAAA
                     SLTTVALSPNTTFDLDVVGRGIAELEIVTNPRGAFQGLTKTRDGSFAYVGNGPKSGRL
                     GPEAFFWPVDGCPVKGPPDAGLDVETGGNDGWTAKIEGQLEAQNATCGAGLRTSLPSV
                     WCASLPAAKPRSEAVVDAPRIRWTLTAKPGVIVGAFDAVLKANASSVDTKKVPFILPG
                     QSVSFETPRTDGRRRRVFTEQACPGCWDLGGVPPWTESAWTVEIGSKR"
     gene            complement(6624..7898)
                     /locus_tag="PLB01_10980"
     CDS             complement(6624..7898)
                     /locus_tag="PLB01_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77870.1"
                     /translation="MKARPAVSVLLLAALARPAAAEIRRDPTGVNVNSQGPMSVYITF
                     GGLVNQTPAEGTWCGELISAAPDIGQKCDPKTIYGSLPARYDRSTLQGGTFTDVMTIP
                     ATVARRAYQAAERGALSPFFYVRRFTSTVGGRDEYVTITCRPAGGGARSPFSLVDVKV
                     AFAIDKPILSVKVGDVAPTLSAQISYTGTGRLKGRWEVVLPYDEPPSTTDLLTEATLP
                     PDQRPNQKRYRELERFNVYLPPTGRTVLPGPDPKRIPTELEGFHDILLRIEAADDRDS
                     ESSLALAGAGAGVVLGGAVAGFPMPALRYYVGSQPAGLFPGASGGLALLSPANRTVAE
                     PGASLTFRWAPVGSTVFYRLEILGGDGVLLHEAYVAAPSTSYAAPTWLAGKAGGAVLK
                     WSVTAQGAEGQDLASSGAAELRFEGSRKGEKP"
     gene            complement(7908..9980)
                     /locus_tag="PLB01_10985"
     CDS             complement(7908..9980)
                     /locus_tag="PLB01_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77871.1"
                     /translation="MRRGLTVNTFGRLVLSVLLGGAAPALGEDLTVRLETGGVDPVPR
                     RFPIEVVPSRPIPASEGRLAVQIGRTDVTDLFEPTDRGLRYRAQVRPLPPGPQEITVF
                     LVPPDGEWKEVARVSIKVLLPGGLERASIAPKLDLTLKGQLGEGHFPDSAAPDRHTFQ
                     DLTGQANVTADLGRGSFGLGAQMGLALAARREDALRYPQLGNDAPYVDLASYGVKVQA
                     GPAAMEWGGVSFGENRHLVNGFSSRGGKGTLALGGVVDVMVAAANGTQIVGWDNFFGL
                     DTREHQVFAGRVGIEFVPSAPGTVRVEGTGVDGSLQPQNSVNRGFVSDAEKSRGVGLR
                     ALLNLFGGRLRLEGGFSRSRFESPPDPTLEQGENVVPIAATTRSASYTVAALDVVKGA
                     GPFSATVTFRHERVDPQYRSVAVSTQADLEQDAAELALTAGPVSVQAAYGWSEDNIAN
                     LPNILKTKTRRPTLAATVPLGPLLGGAAPAWWLPQLTYGLNETHQFGTSLPEGGGFNE
                     TQVPDQLSTNHTGGVDWQAGSVRWGYRVNYSLVDNRQPGRERADALNVAQSANAAVPV
                     AAWLDAAAEYAWERSEAKETGTVDRTRRASATLGIRPFRNGTLAGTGSRTETADDAST
                     RDSLNWNVNVEAGWRFEPPRKGTHGFSGQLSLRYGWATTRSRDLVQGIDTYRKTWVVS
                     SGVSLSIF"
     gene            10047..>10599
                     /locus_tag="PLB01_10990"
     CDS             10047..>10599
                     /locus_tag="PLB01_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQQ77872.1"
                     /translation="MPNRLAGESSPYLLQHAHNPVDWYPWGDEAFARARREGKPVFLS
                     IGYSSCHWCHVMERESFENAAVADLLNEHFVSVKVDREERPDVDDVYMTAVQLTTGRG
                     GWPLSAFLLPDGKPFFAGTYYPPEDRHGRAGFKTLLLRVAQAWGNDRAALEDSARQIA
                     EEVGRAADPAGRVGTAALSRDALL"
BASE COUNT         1527 a         3584 c         3865 g         1623 t
ORIGIN      
        1 tgggacgtcg agtccgacgc gacgcaggac gtcctcgacc gttaccacgt cctcgagccg
       61 ttcgcggggc tcctgcccgt ggacgaccag gtccgcctcg cggaacgatt cgacttcgac
      121 gcactccgct ggggccgcgt cgaggcgatc ttcctgatcg tgatgttcgg gccgctcgcg
      181 ctctcgtcgg tgctgggcgc gcttgcggtg ttcgaggccg cggacgttcc gaggatcgcg
      241 atcttcggcg ggatcaccct cgagcaggtc ctgaggctgc gcaggctcgc ggcgggccgg
      301 ctcgccccga gcgtgctcgg cgtcctcgtc cgcccgttcg cccggaagct cctcgcctga
      361 gtcagccggc ccgcttctcg gcgcgcacga cgcggagcgg agggaagacg tcgaggacgt
      421 gcggccccgt ctgcgtcacg gcgagcccgg ccgccgtgaa ggaccgcgcg tagtcggcgg
      481 tccggatgaa gtccgagacg agcgcgacgc cgcccttctt caggacccgc gcgatctcgt
      541 ggcacgcgtc cgcgcgtccg cccgcatcgg ggatgttgtg gaggcagacg ttcgagagga
      601 cgacgtcgaa agacccgtcg gggaacggca gcgcgcgggc gtccgcgtgc cggacctcga
      661 cgcgctcggc gactccctcg gcctccgcgt ttgcgagaaa cgcgtcggcg gtgttgcccg
      721 tgaggtcctt cgggctccag acgtcgatcc cgacggcggt cccggtcgtg agcctcttcg
      781 cggcgccgag ggcgaggagg ccgcgtcccg tcccgacgtc gaggacgttc tccgcgccgg
      841 tccaggcgac cattgccagc atccgttcgc ggtgccggaa cttcccccac tccgcgtaca
      901 ggatcatgag cgcgccagcg aggcagcagg tgacgccgac ggagacgagc atcccgcgcg
      961 cgttcacgcc cgcgagcggc cacggaagga gggccccgcc caggagggcg aggcctccga
     1021 tcacgaagag gttccggacg acgccggggg cgtcggtgcc gtagtccagc cttcgcacac
     1081 cggaatgcta accggaaggg cggccggtca ctttttcgga agcggtagag ggcccttcgg
     1141 cgcgggcgcc ggcgtgggag gaaccgcgat cgtgaggcga atcgaggccg cgccgctcgg
     1201 gggctcgaca caacggagct tctcgaacgt aaaactctgg cggtcgatcg tcgcggctcc
     1261 gaggagcacg ggcttgaggc gcaccttcag ctcggcgtcg ctcgccgaga gcacggagac
     1321 gtccgtgaag cggccctggc ccgcgaacgg ggtcttgggc cggacgtcca gaagggtctt
     1381 gcccttgagg acgagcgtgt agaggttgcc gggcacgagc tcgaaggccg gactggagcc
     1441 ctgtgcctgc gccgaaggcg tgaccccggg cgccggctct acggcgaatg aatcgatctg
     1501 gggcgggtcg aggacccggg ccttcacccg gtcgaatccc gagacctcca cggggtagtg
     1561 aaggcggact tcgccgtcgc cgagcgccgc gtcggcatca gtcttcagct cgacgtcgat
     1621 ggacgacccg agcccgtgca cccgttgcac gaacctcgcg tggacgcccg ggagagaact
     1681 gagctcaacc tcgctcgcgg tatctaccgc gaacccggag accctgagga tgaccgtccg
     1741 gccccggaag aggggatagg tctcgccctt gttgaagccg gctccggcgc acgtcgtcgt
     1801 cgacccgaag aagtccacgg tggccgcggt tgggcccgcg gcggccagga agacgagcgc
     1861 ggacaggggg acgaggctgg gtctggcggc gtatctcatc ggtcccctca cggcagcttc
     1921 aggttgggcg ggccctcgag gacgagcttc ttgagggtga tccggtaggc ctgcgggtcg
     1981 atcgtcggcc cgtgcgtccc gctgctgtcc gtgagcacgg tcgccatcga gacgcactgc
     2041 attccgagat agaacggcct gatcatcgtc accggcccaa ggaggttgga cttgaacgac
     2101 acgccgtcca cgacgatctc tcggtccccg aacgccatgt gatcggacac gggcaagccg
     2161 gcctccggcc tgatggtggt gacgcccctc ccgaacgagt acttgacgag gtcccccccc
     2221 gtcatcgtgc cctcgaggcc gcagcggtta cccacgtgct tcgagtccca ctggatttcc
     2281 gtcagcgtga cgccttccgg aaggaaccac tccttcgtct ggtatccgca cgagacgtcg
     2341 agaggcccgc cgcggatgac gaccgagaag tcgctgccgt cgtcgtcgat cccgaccctg
     2401 gctccgttcg ggagcgtccc ctcgcagatg cttccgaggc cggaatggac gcgcgccggt
     2461 ttgagcacgg acttgagctg ggccgtgctg gagaacgtgt acgtcacggg ggtcgcgacg
     2521 gtcaggttcc cctcgagcgt cacgcgcgtc atcgacgatc cgtccgccgc gagcggatgg
     2581 aagcggatct cgaggcggca ggttcccggc cgttcgaacc agcccacgac ggcgaagtcc
     2641 agcgaaaggt cctcggtccg gttcgacgtc ccgaacgaga ttcccggctg cttgtggaac
     2701 gagcacgtcc cgccgaacgt cacggcgttt gcgtcgagcc tggcgcccgg gaactcccgg
     2761 accttcacgc ggacggcctt attcatgaag ggatacgcgg tcccgttgac gctcagctca
     2821 gcaagggcgg gcgtcggctg gtcgagcttg tagttcgtct caagcgtctt cgcctgcgtg
     2881 ccgcggtgag ccaccacgaa gatcccggag acgccgagca cgtcggccgg gacgtcgagc
     2941 tcgatgcgcg tcgacgtgct cgaggagacg cggacgggca gccggtactt cgaggggccg
     3001 accgcggcct cgaagtcggc aggccggaag cccgtgcccg tgatcacgat cttgcccccc
     3061 gccacggcgc ggccccgcgg ctgccagtcc gtcacgtcgg gcggcggggg gaggagcgac
     3121 tgcagggcct cggtcgggag cgtcgggcgg ggcgtcgctg ccttgacggg cggtttctgg
     3181 atcggcacct ggccggcagc ggggagaacg gcgagggccg agacgagcgc gagggatcgg
     3241 aggtgtttca tgagttgact cccgtattca cttgaacgcg tcgcgccagc tcttgcctgc
     3301 ggggccgatt accttgacgc tcgagagccg cgccttgaag acgtggctgt ttttcggcag
     3361 gtcgtttccg ttcggcttgc agagggcttc gaacttcacg gcgaagacgg ccgactcgtc
     3421 gttgaagaac gtcaccgtgc tcccgctcga gacctgtgtg gcgaacttgt tgaccgcggc
     3481 gcagagggag tcctccgtga agtcccaatc aaccgacttg acggcccagt cgtcgcggac
     3541 gaggagcgcc ggggaggtct cgaaccggca ggcttcgtcg agagcgccat tgcggagctg
     3601 gaacgtgagg tcgccgccct ccttcacgac gccggtcgag aacgtcccgg ccgtgccgac
     3661 ggagaggagc tggcacggga ggccgccctt gctcgcggtg gccgagagct tcttcccgga
     3721 gggcgtcgtg aactcgagga ggtcccacgt gtcggaaacc gtgtacgtct cgagggaggg
     3781 gacgagcacg ttcggaaggg acagtgccgg cccacgggac ccgtagggtt ggatctccat
     3841 cgggcagttc tttcccgaag cgtcggcgaa gaaactgaag tcgtacttca ggcggtacgg
     3901 gcttccgccg ggttcgctct tgggagccag ccccccggcc cggcagccag gggaccagag
     3961 agagggccgg aagaaggtct ccgccgtggc gtcgaagtcc tcgagctcga cctcgatctt
     4021 cgtgtccttt ccgcgtgtga aggacaggtt gatgtgcggt ccgtccgtga tcttgaagga
     4081 cgcgacgcgc ggcttcttga ggaccttgaa cgatggcgtg agcgtcttct tcggtccgtt
     4141 gacgccgtac cagacgacga gcggcgcgcc cggcgagctg aacgtgtcgg ggacgcgcac
     4201 gtggatgaac gtgtcgcacg aggccgtcgc acagttgccg tgcgcgatga tcgggaggtc
     4261 gacctggccg agccgggcgt ggaattcgct ggaaacgaga tcgcccgtga agacgatctt
     4321 gtccccctgc acgacgtact ccttcgggct ccagttcgag acggtgggct ccccgcgcat
     4381 cgtgatctgc ggcgggcggt ctgccggacc cacgattgcg ggacgcggcg tcggcgtcgc
     4441 cgtcggcagg ggcggcacct tcggcgcagc gggcgtcggg atcttcggcg tcggaatctg
     4501 tgcggaggcg gcgcccgccg tgaggagcag agcgagggcg gcccgggcga tcgcgtcacg
     4561 tgttctcatg cgaatttctc ctcgtcgaat ctcgattcga acccgcgcct cacttccgcg
     4621 gcgcgatcgg cgacaaaggc gtggcccgcg gagtgggcgt cggcgtcgga agcgccggcc
     4681 ggaccccggt cggcggagcg atcgggccga acggaggctt cgtcggcgtg gggctcggcg
     4741 ccggggttcg cgtctgcgtc gccgtcggag tgggcgtcgg tgtgcgcggc ggatcgaaga
     4801 ccgtgaaggc gatggagaat tcgggcatca ggttgaagca gaagcctggc gcgatgaagt
     4861 gccggcttcc gacgtcgcga tctcccgcgg tcgacgtgaa cgagacgcgc agctcgtcgg
     4921 gaagctggag gacgatcttc gcgttgctca ggctggatct gaagaacgcg cggtcgtcga
     4981 tgcggacttc ctgcaggttc gtccccttga ggacgacgac gtgcgggtcg agcgacgtga
     5041 cgcgcgtcgc tccgccggac ttcgacacgc ctccttcgga cgcgaaggag atcgacgtca
     5101 cggagggccc ggcctgcagg ctcgcgttga agacgtcggt gccgccgccg aagtagtgga
     5161 gcgtgacggt gccctcggcc gcgtttccga ctgccggagg ggcaaaccgc agcacgatgt
     5221 tcgacccgag cccgtttttg cgcgacacga ccgtcaccgg cgtgcccggg agcgttgtcg
     5281 tgacggacgt cgcgaggtcg acgccgaagc ccgtcaccgt gacgtcgttg tgggctgcgc
     5341 gtgtcaggag cggggccttg tcggcccaga ccgtgcaggt ctcgacctgg tcgatcgagg
     5401 cgcccgccga tccggccgtg agggcgattc cgagcaggag agcgggtctc gagcgtctcg
     5461 tcaaataccc tccggcggtc atcgcttcga cccgatctcg acggtccacg ccgactcggt
     5521 ccagggcggg acgccgccga ggtcccagca gcccgggcag gcctgctcgg tgaagacgcg
     5581 gcggcggcgc ccgtccgttc gcggcgtctc gaacgagacg gactggcccg ggaggatgaa
     5641 gggcaccttc ttcgtgtcga cactcgaggc gttggccttg aggaccgcgt cgaacgcgcc
     5701 gacgatcacg ccgggcttgg ccgtaagcgt ccaccggatc ctcggagcgt ccacgaccgc
     5761 ctcgcttcgg ggcttcgcgg ccggcagcga cgcgcaccag acggacggga gcgacgtcct
     5821 gagcccggca ccgcacgtgg cgttctgcgc ttcgagttgg ccttcgatct tcgccgtcca
     5881 cccgtcgttt ccgccggtct cgacgtcgag gcccgcgtcc gggggacctt tcacgggaca
     5941 gccgtcgacg ggccagaaga aggcctcggg cccgaggcgc ccgctcttcg gtccgttgcc
     6001 gacgtacgcg aacgacccgt cccgggtctt cgtcaggccc tggaaagcgc cgcgaggatt
     6061 cgtcacgatc tcgagctccg caatcccgcg tccgaccacg tcgaggtcga aggtggtgtt
     6121 tggggaaagc gccacggtcg tcaggcttgc agcagccggg atcgcgagcc ggatgcggtc
     6181 gatttgcggt cgcgccgcga ggcgcacgaa gagttcgccg agcgtcgccg acccctgtac
     6241 gatccggacg atggccgggg gcgacgatcg ccgcggcgga tcaccgcgcc ggtagagcaa
     6301 gcggatcttc atcgccttcg cggacgactc caggacggcg ccctcgacgc cgtcgggcgt
     6361 cacgacccgc ggtcccgggg ccgcctgctt gggggaggac ccgcctccgg tcgcgccttc
     6421 gagcccgctg cccgcgatct cgaggtcgag tggctcgccg aagatcagcg tcaccgggtc
     6481 cgtcggtcca gccgtcagca tggccctgca ggtccccggg taggagtact tgatggactg
     6541 cacggttccc gccgcgggca tgccggccgc ggccgacagg aggagggcgg aaacggcgaa
     6601 ggcgggtctg cgggcgctgt tcctcaaggc ttctctcctt ttctacttcc ttcgaaacgc
     6661 agctcggcgg cgccgctcga cgcgaggtcc tgcccctcgg cgccctgcgc cgtcacgctc
     6721 cacttgagga ccgcgccgcc cgccttgccc gcgagccacg tcggagccgc gtaggaggtg
     6781 gacggcgccg cgacgtacgc ctcgtggagg agcactccgt cgccgcccag gatctcgagc
     6841 cggtagaaca cggtcgagcc gacgggcgcc caccggaacg tgagggacgc gcccggctcg
     6901 gcgactgtgc ggttcgcggg ggagaggagc gcgaggccgc cgctggcgcc cgggaagagc
     6961 ccggcgggct ggctcccgac gtagtagcgg agcgccggca tcgggaagcc cgcgaccgcg
     7021 ccgccgagga cgacgccggc gcccgcgccc gcgagcgcga gactcgactc cgagtcgcgg
     7081 tcgtcggcgg cctcgatcct gaggaggatg tcgtggaagc cctcgagctc ggtcggaatg
     7141 cgcttcgggt cgggccccgg aaggaccgtg cggcccgtgg gcggaaggta gacgttgaag
     7201 cgctcgagct cacggtagcg cttctggttc ggccgctggt cgggcggcag cgtcgcctcc
     7261 gtgaggaggt cggtcgtcga ggggggctcg tcgtacggga gcacgacctc ccagcgcccc
     7321 ttgaggcggc ccgtgcccgt gtaggagatc tgcgccgaga gggtgggggc gacgtcgccg
     7381 accttcaccg agagaatcgg cttgtcgatc gcgaaggcga ccttcacgtc gacgagggag
     7441 aacggcgagc gcgcgccgcc gcccgcgggg cggcacgtga tcgtgacgta ctcgtcgcgc
     7501 ccgccgaccg tgctcgtgaa gcggcggacg taaaagaacg gcgagagggc tccgcgctcg
     7561 gccgcctggt aggcgcggcg ggcgaccgtg gccgggatcg tcatgacgtc cgtgaacgtg
     7621 ccgccctgca gggtcgagcg gtcgtaccgc gcggggaggc tgccgtagat cgttttcggg
     7681 tcgcacttct ggccgatgtc cggcgcggcg gagatcagct cgccgcacca cgtcccctcg
     7741 gcgggcgtct ggttcacgag gcctccgaac gtgatgtaca cggacatcgg cccctggctg
     7801 ttgacgttga cgcccgtggg gtcgcggcgg atctcggccg ccgcggggcg cgcgagcgcg
     7861 gcgagaagaa ggacggagac ggccgggcgc gctttcacga aggcctccta gaagatgctc
     7921 aggctgacgc cggagctcac gacccaggtc ttgcggtacg tgtcgatgcc ctggacgagg
     7981 tcgcgcgagc gcgtggtcgc ccagccgtat cgcagcgaga gctgacccga gaacccgtgc
     8041 gtcccttttc gcggtggctc gaagcgccag cccgcctcga cgttcacgtt ccagttcagg
     8101 ctgtcgcgcg tcgacgcgtc gtcggccgtc tccgtgcggg acccggtgcc ggcgagcgtc
     8161 ccgttccgga agggcctgat cccgagcgtc gccgaggcgc gccgcgtgcg gtccacggtg
     8221 cccgtctcct tcgcctcgga gcgctcccag gcgtactcgg ccgcggcgtc gagccacgcg
     8281 gccaccggaa cggcggcgtt cgccgactgc gccacgttga gcgcgtccgc gcgctcgcgg
     8341 cccggctggc gattgtcgac gagcgagtag ttcaccctgt agccccagcg caccgagccc
     8401 gcctgccagt cgacgccgcc cgtgtggttg gtcgagagct ggtcggggac ctgcgtctcg
     8461 ttgaagccgc cgccttcggg caggctcgtc ccgaactggt gcgtctcgtt cagcccgtag
     8521 gtgagctgcg gaagccacca tgcgggcgcc gcgccgccga ggaggggccc gaggggcacc
     8581 gtggccgcga gcgtcggccg ccgtgtcttc gtcttgagga tgttcggcag gttcgcgatg
     8641 ttgtcctcgg accacccgta ggccgcctgc acggaaaccg gccccgccgt gagggcgagc
     8701 tcggcggcgt cctgctcgag atcggcctgg gtcgagacgg cgacgctccg gtactgcggg
     8761 tccacgcgct cgtgccggaa cgtgaccgtc gcgctgaacg ggccggcgcc cttcacgacg
     8821 tcgagagccg cgaccgtata cgaagccgag cgcgtcgtcg ccgcgatcgg cacgacgttt
     8881 tctccctgct cgagtgtcgg gtccggcggg ctttcgaagc ggctccgcga gaagccgcct
     8941 tcgaggcgca gccggccgcc gaagagattc aggagcgcgc ggaggccgac gccccggctc
     9001 ttctcggcgt cgctcacgaa accccggttg acggaattct ggggctggag cgacccgtcc
     9061 acgccggtgc cctccacgcg caccgtaccg ggggcggagg gcacgaactc gatgccgacg
     9121 cgacccgcga acacctggtg ctcgcgcgta tcgaggccga agaagttgtc ccagccgacg
     9181 atttgcgtgc cgttcgcggc cgcgaccatg acgtcgacga cgccgccgag ggcgagcgtc
     9241 cccttcccgc ctcggctcga gaagccgttc acgaggtgac ggttctcgcc gaacgagacg
     9301 ccgccccact ccatcgcggc cggtccggcc tggaccttga cgccgtagct cgcgaggtcg
     9361 acatacgggg cgtcgtttcc gagctggggg taccgcaacg cgtcttcgcg gcgggccgcg
     9421 agcgccagtc ccatctgggc tccgaggccg aaggagcccc ggccgaggtc cgccgtgacg
     9481 tttgcctgcc ccgtgaggtc ctggaacgtg tgccgatcgg gcgccgccga gtcggggaag
     9541 tggccctctc cgagctggcc cttgagcgtc aggtcgagct tcggggcgat gctggcgcgt
     9601 tcgagcccgc ccgggaggag caccttgatg ctcacccggg caacctcctt ccattcgccg
     9661 tcgggcggga cgaggaagac cgtgatctcc tgcggccccg ggggaagcgg gcggacctgc
     9721 gcgcggtagc ggaggccccg gtccgtcggc tcgaagaggt ccgtcacgtc cgtccggccg
     9781 atctggacgg cgagacgccc ctcggacgcg ggaatcggcc ggctcggcac gacctcgatc
     9841 gggaaccgcc gcggcacggg gtcgacccct ccggtttcga ggcgcacggt caggtcttcg
     9901 ccgagcgcgg gcgccgctcc cccgagaagg acggacagaa cgagacggcc gaacgtgttc
     9961 acggtgagtc ctcgtctcat ggctttcttg caccggaacg cgcaggacgg cgcccggagg
    10021 ggtcacgccg ggtccagaat ccgggaatgc caaaccgcct cgccggcgaa tcctcgccct
    10081 acctcctcca gcacgcgcac aacccggtgg actggtaccc gtggggcgac gaggccttcg
    10141 cgcgggcccg gcgcgagggc aagcccgtct tcctctcgat cggctactcc tcctgccact
    10201 ggtgccacgt catggagcgg gagtcgttcg agaacgcggc cgtcgcggac ctcctgaacg
    10261 agcacttcgt gtcggtcaag gtcgaccgcg aggagcgccc cgacgtggac gacgtctaca
    10321 tgacggccgt gcagctcacc accgggcgcg gcggctggcc gctctcggcg ttcctcctgc
    10381 cggacggcaa gccgttcttc gcggggacgt actatccgcc cgaggaccgg cacggacgcg
    10441 ccgggttcaa gacgctcctc ctccgggtcg cgcaggcctg gggcaacgac cgcgcggccc
    10501 tcgaggactc ggcccggcag atcgcggagg aggtcgggcg cgcggcggac ccggccggcc
    10561 gcgtcggcac cgcggccctc tcgcgcgacg cgctgctcc
//
LOCUS       DAOMFY010000122        10581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1925_length_10581_cov_6.870321, whole genome shotgun sequence.
ACCESSION   DAOMFY010000122 DAOMFY010000000
VERSION     DAOMFY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10581
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_10581_cov_6.870321"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PLB01_10995"
     CDS             <1..1022
                     /locus_tag="PLB01_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ77873.1"
                     /translation="PEPPAAGGQAPGPLGLRGWQAAFLAVGLPGLVLAAWVRSLREPA
                     RAAAHPPLTATPVRTFLRESASVLPPFTLFTLAAAGAGAAGVLVNVAASAALGGLAWL
                     LTAALGNPAQWIALAAGFYAVFSWAQALRLTSREDFDLVFKNRALVLANLGFGLVAFG
                     TYGTSFWIAPYFVRVHHLNLAKTGLLLGGISAAGGWLGATCGGLLADAWGKRRADGRL
                     LVGAASALVPIPLALGMLAAPSLAPAAALALPITVLTALWVAPATTTAQELVPPRLRG
                     TASAVLLLHTTFLGLALGPYTIGRLSVYAGSLKAALALALPVNLAAGLLLLLAARALP
                     RPSAS"
     gene            1127..1612
                     /locus_tag="PLB01_11000"
     CDS             1127..1612
                     /locus_tag="PLB01_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQQ77874.1"
                     /translation="MKTSMGSITIELDAAKAPETVKNFLAYVNAKHYDGTVFHRVIDG
                     FMIQGGGFAVDKTQKKTNAPIKNESSNGLQNKVGTLAMARTGDPNSATAQFFINVKDN
                     GFLDKAQSQDGYGYCVFGKVTDGMDVVNKIKATPTSNQGGAFVNAPVTPVVIESVRVQ
                     P"
     gene            1631..2800
                     /locus_tag="PLB01_11005"
     CDS             1631..2800
                     /locus_tag="PLB01_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQQ77875.1"
                     /translation="MSTLPPPYGYVPPPPRRRGGPIVLPVLLGLLAGWLVFTLMQRTP
                     GGGAAPPRLKGAPAEARPVTARGDLASDEKATIELFERCSPSVVYITSLARRPVSFFE
                     MTEVPQGSGSGFVWDRQGHIVTNFHVIQDASSLVVTLADQSDWKATVVGVEPDKDLAV
                     LRIGAPEAKLPPILVGTSRDLKVGQKVFAIGNPFGLDETLTTGIVSALGRTIESVTGR
                     KIQGVVQTDAAINPGNSGGPLLDSAGRLIGVNTQIASPSGASAGIGFAVPADTVNEVV
                     PQLIAHGRVIRPRLGITPAQEGVVRRLGLTGVLVLEVQEGTGAAKAGLRGTRRERDGS
                     IALGDVVVGVAGKRVASYDDLVSALEKQKIGDTVPVRVLRDGREITVDVTLSASR"
     gene            complement(2778..3572)
                     /locus_tag="PLB01_11010"
     CDS             complement(2778..3572)
                     /locus_tag="PLB01_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HQQ77876.1"
                     /translation="MCLIVLAWKASPSVRLAVAANRDEFFARPAAAAAWWRDAPDVLA
                     GRDLRGGGTWMGVTRSGRFAALTNFRDATAPRKGDAPSRGNLVGDFLRKEEGPAAFLS
                     RLAWNADTFEGFNLLVSDGGELWSFSNAEERGPVALSPGVYGLSNHLLETPWPKVAAA
                     RERLAAAVATEAAPPELEASLLALLRNRALAPDGALPETGLPLAWERALSAAFVVLPD
                     YGTRASTVLVVGADGEARFTERTFGPGGAPLGEVRETFRLSATPRA"
     gene            complement(3583..3954)
                     /locus_tag="PLB01_11015"
     CDS             complement(3583..3954)
                     /locus_tag="PLB01_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77877.1"
                     /translation="MRRRARGSSLLLVLGILTVFGLVLGVLATVLVTDLSSSRRRANA
                     AYAAELARSGTDWARGALLARGGLPSATLRVERGEIAVATEELPDGRLRVVSTGRVRG
                     GDATLAARREVTVLSASARAR"
     gene            complement(3951..4442)
                     /locus_tag="PLB01_11020"
     CDS             complement(3951..4442)
                     /locus_tag="PLB01_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQQ77878.1"
                     /translation="MTRRGFSLVEMCVVVMIGLVLLFALLPVALGLLRQQAGLNARSL
                     GIDTWPLLVERLGGDAGRSSGALVSPPFPAAAFRLRFPPARDEDPDVTWTFGDGRAER
                     ATERRKPDGEVVRATTTWDLPGTLALEPDELENGRLLFAWNDGSGPELVAIACGRPTE
                     PAR"
     gene            complement(4439..4837)
                     /locus_tag="PLB01_11025"
     CDS             complement(4439..4837)
                     /locus_tag="PLB01_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77879.1"
                     /translation="MAAPRSRGVFLLEALVSLAILGIVLTLGASFLARRRALEADRRD
                     HDRAARALASEWVYLRTAFRHDLFPKDRRTFVGPGAFVDALDARAPVLAVRQTDVDGL
                     YRVTLDIGWGVGGKKRLVEEGFVFRGVSPP"
     gene            complement(4846..5463)
                     /locus_tag="PLB01_11030"
     CDS             complement(4846..5463)
                     /locus_tag="PLB01_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77880.1"
                     /translation="MKRPSAAPRAARVVLALSAASVLYGCASGSSAPPPSAGGAKKRL
                     VLVEARGPGPEIERFVPRLLSAASDRGLGAIVDARLSGAHLADVAAAGEISAAFRREY
                     PGDVYIGALMSPCTIQRHEGRVYDRQQTPGGVPVRTETVVVTYDAECSVVLTLVGEAG
                     AAVKTIDVTGRNGSAEVSASNTEAEGQAAADAADRAAKKLASFVR"
     gene            complement(5460..6494)
                     /locus_tag="PLB01_11035"
     CDS             complement(5460..6494)
                     /locus_tag="PLB01_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77881.1"
                     /translation="MRRAALLGLFALAASASAADEEKPAARIVRTEQVVRLVVDAYVT
                     DKRGDPITDLVASDFRVTADGRPLEVESVEFVAAGTPEGPVAMPPPGDGASASAPAPA
                     PYPPGRLIVLFVEGDLGRQRTKGLMRVKQELHRLLDRLKPADRVAVVGFFSHLTVLTD
                     FTNDRATLDEAFRLGLGSSRDRDVRISPYPSLAANLDFAEARKAAHVERALELVAMAL
                     APIPGAKAVVFAGWGFFANRAPIEGVLYDRALGALMRARANVFMLDTSDSDWHTLETA
                     LQVASQATGGTYAKTNLFAGQAVERVARALAGRWVLVCVAPDAAPRTLDVSLVQRRGD
                     VVARAYTALP"
     gene            6525..7553
                     /locus_tag="PLB01_11040"
     CDS             6525..7553
                     /locus_tag="PLB01_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HQQ77882.1"
                     /translation="MNAAVPPPTDLLVVGAGPAGLACALEATRAGLTHVVLEKGALLN
                     TLVNWPVHVVFFSTPELLEIGDHPFVSAGPKPTRREGLDYYRKVAERERLDVRLGAPV
                     TSLTKADGIFAAATPKGTFTARAAVVATGFFDTPNLLGVPGEDLPKVSHYYREPYAHA
                     RTDVLVVGGKNSAVEAALDLHRHGARVTMAVRGPALGASVKYWLKPDVENRIREGSIR
                     AFFETTVARIDEESVTLLGSGGTTELPNDFVYALTGYRPDFSFLTAAGVGITGDLEVV
                     HDAGTMETGVPGLYVAGVVAAGRHIGRLFIENGRAHAAWIVGHVLAQRGATGRPVVPG
                     IRRFQDGD"
     gene            7556..8170
                     /locus_tag="PLB01_11045"
     CDS             7556..8170
                     /locus_tag="PLB01_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77883.1"
                     /translation="MAVGRVGAVLALAAAGAAAVLVTPAVKRTIALGDRAKGAPVSFL
                     AGRGFTLELPTGRWYEARVPRFELTDGGDPADPNRPALEHRLLRAGEPAQLAVISWTL
                     LGGGSLDLDNAAALVSEGSARRLEKWKLLGVAPLPGRGATRVLHATALLEGRDVELLW
                     GLYPNGPTVYGVLVAAETSAFVRLRREMEGILASVRAFDAPGSG"
     gene            complement(8189..9220)
                     /locus_tag="PLB01_11050"
     CDS             complement(8189..9220)
                     /locus_tag="PLB01_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HQQ77884.1"
                     /translation="MKRLIKGLVLVLSVLAVIVGAGIAYLAAKKPAQRPASAEKIEAT
                     PARLARGEYLAMHVVDCLLCHSDAKFDRFGLPRIPGTEGKGGFAFTKEFGIPGVLYAR
                     NITPDPETGIGKWTDGEILRAIREGVDRDGNTLFPMMPYAAYHEMSDEDARAIVLYLR
                     TLRPIRNAVPAKKIDFPVNLLVKSAPKPVAGPVVTPDPKTDHIGYGKYLTVIGGCLGC
                     HTPETRPGAPIPGREYTGGRLFTGPWGRNVTPNLTPHKDAYVGRATKEEFIGRFRSFA
                     GLTGDAAPVASKGRNTVMPWLALSGATDEDLGAIYDFLKTLPPVENKVNPFPDAPDVA
                     PEAPKTAER"
     gene            complement(9251..>10581)
                     /locus_tag="PLB01_11055"
     CDS             complement(9251..>10581)
                     /locus_tag="PLB01_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77885.1"
                     /translation="VASSRGSLSGCSLGGASTASAPAAPPASKLGAQKAAAIANGYAG
                     GHLDFGRRQWETSGDGFQRAFAFNTKDDAAAYLAATAWAHAGNAPAALEWLDQVWRLN
                     SCLVPRPKSFEGIASDPRFNDALAVIRAQAPKTHRSAVAFTLGARDLVPADLAWDAAA
                     KVFYVASLRKRKIVRVTPGAAGAPAAMADFAGSDGTALDAVLGLKVDTARRRLWAVTA
                     GDPAMEGFRPEDYGRSQLVAFDLATGAILGRWSPLTRPPHQFGGLAVDAAGNVWVTDT
                     ASGEIHVLKAGAEELAVAAAAGAFVAPKSVAFSADGARVWVSDLARGVYRLDAATGAA
                     TLLDQPPGPWPAGLDGLVFHRNALVGVQGTVSVGRVGRWTLEPDGNSFTGMEILDCAH
                     PSYRVPVGGTAVGDDYVYVANSQVDAFGPDGVLPSEKVEDLVFLRLPLGR"
BASE COUNT         1464 a         3939 c         3760 g         1418 t
ORIGIN      
        1 accctgagcc ccctgctgcc ggagggcaag ccccagggcc gctcggcctg cgcggctggc
       61 aggccgcgtt cctcgccgtc ggcctgcccg gcctcgtcct cgccgcctgg gtgcgcagcc
      121 tccgcgagcc cgcccgcgcc gcggcgcacc cgccgctcac ggcgacgccc gtgcggacgt
      181 tcctccgcga atcggcctcg gtgctcccgc cgttcacgct cttcacgctc gctgcggcgg
      241 gcgccggcgc ggcgggcgtc ctcgtcaacg tcgcggcctc tgccgcgctc ggcggcctcg
      301 cgtggctcct cacggccgcg ctcggaaacc ccgcgcagtg gatcgcgctc gccgccggct
      361 tttacgccgt cttctcgtgg gcgcaggcgc tgcgcctgac gagccgcgag gacttcgacc
      421 tcgtcttcaa gaaccgcgcg ctcgtcctcg cgaacctcgg cttcggcctc gtcgccttcg
      481 ggacgtacgg gacgagcttc tggatcgcgc cgtacttcgt gcgggtccac cacctgaacc
      541 tcgcgaagac gggcctcctc ctcggcggca tctcggccgc cggaggttgg ctcggcgcga
      601 cctgcggcgg gctcctcgcg gacgcgtggg gcaagcggcg cgcggacggc cgcctcctcg
      661 tcggcgcggc ctcggccctc gttccgatcc cgctcgcgct cgggatgctg gcggcccctt
      721 cgctcgcgcc cgcggccgcg ctcgcgctcc cgatcaccgt cctcaccgct ctctgggtcg
      781 cgcccgcgac gacgacggcg caggagctgg ttcctccgcg gctcaggggc acggcgagcg
      841 ccgtgctcct cctgcacacg acgttcctcg gcctcgccct cgggccgtac acgatcggac
      901 gcctcagcgt ttacgccggc agcctgaagg ccgcgctggc cctcgcgctc ccggtcaacc
      961 tcgcggcggg gctgctgctc ctcctcgcag cccgcgcact gccccgcccc tcggcgtcgt
     1021 aagatcccgc cccatgaaaa gactcgcacc cgccctggcc ctcgcccttg tgttcgccac
     1081 ctctgccgtg aagggagaaa cccccgtgaa gaatccgacg gtcgtgatga agacctcgat
     1141 gggctcgatc acgatcgagc tcgacgccgc caaggcgccc gagaccgtga agaacttcct
     1201 cgcctacgtg aacgcgaagc actacgacgg cacggtgttc caccgcgtca tcgacggctt
     1261 catgatccag ggcggcggct tcgcggtcga caagacgcag aagaagacga acgcccccat
     1321 caagaacgag tcgtcgaacg gcctccagaa caaggtcggg acgctcgcga tggcgcgcac
     1381 gggcgacccg aacagcgcga cggcgcagtt cttcatcaac gtgaaggaca acgggttcct
     1441 cgacaaggcc cagtcgcagg acggctacgg ctactgcgtc ttcggcaagg tcacggacgg
     1501 catggacgtc gtgaacaaga tcaaggcgac gccaacctcg aaccagggcg gcgcgttcgt
     1561 caacgccccc gtcaccccgg tcgtcatcga atcggtccgc gtccagccct gacgcgccgg
     1621 gcgcgtccgc gtgtcgaccc tcccgcctcc ctacggctac gtcccgccgc ctccacggcg
     1681 gcggggcggc cccatcgtct tgcccgtcct cctcgggctg ctggcgggat ggctcgtctt
     1741 cacgctcatg cagcggacgc ccggcggcgg cgccgccccg ccgcggctaa agggggctcc
     1801 cgccgaggcg cggcccgtga ccgcgcgcgg cgacctcgcc tccgacgaaa aggccacgat
     1861 cgagctcttc gagcgctgca gcccgtccgt cgtctacatc acgagcctcg cccgccgtcc
     1921 ggtcagcttc ttcgagatga cggaggtccc gcagggctcc ggttccggct tcgtgtggga
     1981 ccggcagggc cacatcgtca cgaatttcca cgtgatccag gacgcctcgt ccctcgtcgt
     2041 gacgctcgcg gaccagtccg actggaaggc gacggtcgtc ggtgtcgagc ccgacaagga
     2101 cctcgcggtc ctgcgcatcg gcgcgcccga ggcgaagctc ccgccgatcc tcgtcgggac
     2161 gagccgcgac ctgaaggtcg ggcagaaggt cttcgcgatc ggaaacccgt tcggcctcga
     2221 cgagacgctc acgacgggca tcgtgagcgc gctcgggcgg acgatcgagt ccgtcacggg
     2281 ccggaaaatc cagggcgtcg tccagacgga cgcggcgatc aaccccggga actcgggcgg
     2341 gccgctcctc gacagcgcgg gccgcctcat cggcgtcaac acccagatcg cgagcccctc
     2401 gggtgcgagc gccggcatcg gcttcgcggt cccggccgac accgtgaacg aagtcgtccc
     2461 gcagctcatc gcgcacgggc gcgtcatccg gccgcgcctc ggcatcacgc ccgcgcagga
     2521 aggcgtcgtc cggcggctcg gcctcacggg ggtgctcgtc ctcgaggtgc aggaaggcac
     2581 cggcgccgcg aaagcaggcc tccgcggcac gcggcgggaa cgggacggat cgatcgccct
     2641 cggcgacgtc gtcgtcggcg tcgcggggaa gcgggtcgcg agctacgacg acctcgtctc
     2701 cgcgctcgag aagcagaaga tcggcgacac cgtgcccgtg cgcgtcctcc gggacggccg
     2761 cgagatcacg gtcgacgtca cgctctcggc gtcgcgctga gccgaaacgt ctcccgcacc
     2821 tcgccgagcg gagcgccgcc cggaccgaag gttcgctccg tgaaccgcgc ctctccgtct
     2881 gcgcccacga cgaggacggt cgaggcgcgc gtcccgtagt ccggaaggac gacgaaagcg
     2941 gccgagagag cgcgctccca ggcgagcggg agtcccgttt ccggcagcgc gccgtccggt
     3001 gccagggcgc gattccttag aagggcgaga aggctcgctt cgagctcggg cggcgccgcc
     3061 tcggtcgcga cggccgccgc gagccgctcc cgcgccgcgg cgaccttcgg ccaaggcgtc
     3121 tcgaggaggt ggttcgagag gccgtagacg cccggagaga gagcgaccgg gcctctttcc
     3181 tcggcattcg agaaggacca gagctccccg ccgtccgaga cgaggagatt gaatccttcg
     3241 aaggtgtctg cattccaggc gaggcgtgaa agaaaagccg ccggcccttc ttcctttcta
     3301 agaaaatctc cgacgaggtt tccccgcgac ggagcgtcac ccttccgcgg cgccgtcgcg
     3361 tcgcggaagt tcgtgagcgc cgcgaagcgc cccgagcgcg tcacgcccat ccacgtgccg
     3421 ccgcctcgaa gatcgcggcc cgcgaggacg tccggcgcat ccctccacca ggccgcggcg
     3481 gcggcgggac gcgcgaagaa ctcgtcgcgg ttcgcggcga cggcaaggcg tacggagggc
     3541 gaggccttcc acgccaggac gatcaggcac atcggcgcat tctcaacggg ccctcgcgga
     3601 ggccgagagc acggtcacct cgcgccgcgc ggcgagcgtc gcgtctccgc ctcgcacgcg
     3661 gcccgtcgag acgacgcgca ggcggccgtc cggcagctct tccgtcgcga cggcgatctc
     3721 cccgcgctcg acgcggagcg tcgcggacgg caggccgccg cgcgcgagga gcgccccgcg
     3781 cgcccagtcc gtgcccgagc gcgcgagctc cgcggcgtag gcggcgttcg cgcggcgcct
     3841 cgacgaggag aggtccgtga cgaggacggt cgcgaggacg ccgaggacga gcccgaagac
     3901 cgtcaggatc ccgaggacga ggaggaggga gcttccccgc gcgcgccgcc tcaccgcgcg
     3961 ggctccgtcg ggcgcccgca ggcgatcgcg acgagctccg ggccggagcc gtcgttccac
     4021 gcgaagagca gccggccgtt ctcgagctcg tccggctcga gcgcgagcgt ccccggcagg
     4081 tcccacgtcg tcgtggcccg gacgacctcg ccgtcgggct tccggcgctc ggtcgcgcgc
     4141 tcggcccgcc cgtccccgaa cgtccatgtc acgtccgggt cctcgtcgcg cgccggcggg
     4201 aagcgcaggc ggaacgccgc ggccgggaac ggcggcgaga cgagggcgcc cgaagagcgg
     4261 cccgcgtcgc cgccgagccg ttcgacgagg agcggccacg tgtcgatgcc gaggctgcgc
     4321 gcgttgaggc ccgcctgctg ccggagcagg ccgagggcga ccggcaggag cgcaaagagc
     4381 agaacgaggc cgatcatgac gacgacgcac atctcgacga gggagaagcc gcggcgggtc
     4441 acggcggcga gaccccgcgg aagacgaagc cttcctcgac gaggcgcttc ttcccgccga
     4501 cgccccaccc gatgtcgagc gtcacgcggt acaggccgtc cacgtccgtc tgccggacgg
     4561 cgaggacggg cgcgcgcgcg tccagcgcgt ccacgaaggc gcccggcccg acgaacgtgc
     4621 gcctgtcctt cggaaagagg tcgtgccgga acgccgtgcg gaggtagacc cactcgctcg
     4681 cgagcgcgcg ggccgcgcgg tcgtggtcgc gccggtccgc ctccagcgcg cgccgccgcg
     4741 cgaggaagct cgcgccgagc gtcaggacga ttccgaggat cgcgagcgac acgagcgcct
     4801 cgaggagaaa cacaccgcgg gagcgcggcg ccgccacggc cgacctcagc gcacgaacga
     4861 ggcgagcttc ttggcggccc gatcggccgc gtctgcagcg gcctgcccct cggcctcggt
     4921 gttcgaggcg ctgacctcgg cgctcccgtt gcgcccggtg acgtcgatcg tcttcacggc
     4981 ggccccggcc tcgccgacga gcgtgaggac gaccgagcat tccgcgtcgt acgtgacgac
     5041 gacggtctcc gtccgcacgg gcacgccgcc gggggtctgc tgccggtcgt acacgcggcc
     5101 ctcgtgccgc tggatggtgc acggcgacat cagggcgccg atgtagacgt cgcccgggta
     5161 ttccctgcgg aacgcggccg agatctcgcc ggcggcggcg acgtccgcga ggtgcgcgcc
     5221 ggagagccgc gcgtccacga tggcgccgag cccgcggtcg ctcgcggccg agagcagccg
     5281 cgggacgaag cgctcgatct cgggtcccgg gcccctggct tccacgagga cgaggcgctt
     5341 cttcgcgccg ccggcggatg gcggcggcgc ggacgagccg gaggcgcagc cgtagaggac
     5401 cgaagcggcg gaaagggcca ggacgacacg tgcggctcgg ggcgcggcgc tcgggcgttt
     5461 catggcagcg cagtgtacgc ccgcgcgacg acgtctccgc ggcgctgcac gagggacacg
     5521 tcgagcgtgc gcggcgcggc gtcgggcgcg acgcatacga ggacccatcg ccccgcgaga
     5581 gcgcgcgcga cgcgctccac ggcctggccc gcgaacaggt tcgtcttcgc gtacgtcccg
     5641 cccgtcgcct gcgaggcgac ctggagagcc gtctcgagcg tgtgccagtc cgagtccgac
     5701 gtgtcgagca tgaacacgtt cgcccgcgcg cgcatgagcg cgccgagcgc gcggtcgtag
     5761 aggacgccct cgatcggagc gcggttcgcg aagaagcccc agcccgcgaa gacgacggcc
     5821 ttggcgcccg gaatcggggc gagcgccatg gcgacgagct ccagcgcgcg ctcgacgtgc
     5881 gcggctttcc gcgcctcggc gaagtcgaga ttcgccgcca gagacggata cggagagatc
     5941 ctgacgtcgc ggtcgcggct cgagccgagg ccgagccgga acgcttcgtc gagggtggcc
     6001 cggtcgtttg tgaagtccgt gaggaccgtc aggtgggaaa agaagcccac cactgcgaca
     6061 cggtccgccg gcttcaggcg gtcgaggagg cggtggagct cctgcttcac gcgcatgagg
     6121 cccttcgtgc gctgccggcc gaggtcgccc tccacgaaga ggacgatgag ccgtcccggc
     6181 ggatagggag cgggtgccgg cgcggacgcc gacgcgccgt cccccggcgg gggcatggcg
     6241 accgggccct ccggcgtgcc cgccgccacg aactcgacgg actcgacctc gagcggcctg
     6301 ccgtccgccg tgacgcggaa gtccgacgcc acgagatccg tgatcgggtc gccccgcttg
     6361 tccgtcacgt acgcgtcgac gacgagcctc acgacctgct cggtcctcac gatgcgcgcc
     6421 gcgggcttct cctcgtccgc ggcggacgcg gaggctgcga gcgcgaaaag cccgaggagg
     6481 gcggcccggc gcacggaagg attctcctcc cgtatgctcc gcccgtgaac gccgccgtgc
     6541 cccctccgac ggatctcctc gtcgtcggcg ccggcccggc cggcctcgcg tgtgcgctcg
     6601 aggcgacgcg cgccggcctc acgcacgtcg tcctcgagaa gggcgcgctg ctcaacacgc
     6661 tcgtgaactg gccggtccac gtcgtcttct tctcgacgcc cgagctcctc gagatcggcg
     6721 accacccgtt cgtctcggcc gggccgaaac ccacgcggcg ggaaggcctc gactactacc
     6781 gcaaggtcgc cgagcgcgag cggctcgacg tgcgcctcgg agcgcccgtc acctcgctga
     6841 cgaaggccga cgggatcttc gccgccgcaa cgccgaaggg cacgttcacg gcccgcgcgg
     6901 ccgtcgtcgc gaccgggttc ttcgacacgc cgaacctcct cggcgtcccc ggcgaggatc
     6961 tcccgaaggt ctcgcactac taccgcgagc cctacgccca cgcccggacg gacgtcctcg
     7021 tcgtgggcgg caagaactcg gccgtcgagg ccgcgctcga cctccaccgg cacggcgccc
     7081 gcgtgacgat ggcggtccgg ggcccggcgc tcggggcgag cgtcaagtac tggctcaagc
     7141 cggacgtcga gaaccgcatc cgcgagggct cgatccgcgc gttcttcgag acgacggtcg
     7201 cgcggatcga cgaggaatcg gtcacgctcc tcggcagtgg gggcacgacg gagctcccga
     7261 acgacttcgt ctacgcgctg accggctatc gccccgactt ctcgttcctc accgccgccg
     7321 gcgtcggcat caccggggac ctcgaggtcg tccacgacgc cgggacgatg gagacgggcg
     7381 tgccgggcct ctacgtcgcg ggcgtcgtgg cggccggacg gcacatcggc cggctcttca
     7441 tcgagaacgg ccgcgcccac gccgcgtgga tcgtcggtca cgttctcgcg cagcgcggcg
     7501 cgacgggaag gccggtcgtc ccggggatcc gccgcttcca ggacggagac tgaccatggc
     7561 cgtcggtcga gtcggagccg ttctggccct cgcggcggcc ggcgccgccg cggtcctcgt
     7621 cacccccgcc gtgaagcgga cgatcgccct cggcgaccgg gccaaggggg ccccggtctc
     7681 gttcctcgcg ggccgcggct tcacgctcga gctgccgacg ggccgctggt acgaggcgcg
     7741 cgttccgcgg ttcgagctca ccgacggcgg agacccggcg gaccccaacc gcccggcgct
     7801 cgagcaccgg ctcctgcgcg cgggcgagcc ggcccagctc gccgtcatct cgtggacgct
     7861 cctgggcgga ggcagcctcg acctcgacaa cgccgccgcg ctcgtctcgg agggatcggc
     7921 gcggcgcctc gaaaagtgga agctcctcgg cgtcgcgcct ctgcccgggc gtggagcgac
     7981 gcgcgtcctg cacgcgacgg cccttctcga ggggcgggac gtggagctcc tctggggtct
     8041 ctacccgaac ggcccgaccg tctatggcgt gctcgtcgcc gcggagacgt ccgccttcgt
     8101 gaggctgcgc cgcgagatgg aaggcatcct cgcctccgtc cgcgcgttcg acgcgcccgg
     8161 atccggctga gcccggcgga gcggtcgttc acctttccgc ggtcttcggc gcctccggcg
     8221 ccacgtcggg cgcgtccggg aacgggttga ccttgttctc gaccggcggc agcgtcttca
     8281 ggaagtcgta gatcgcgccg aggtcctcgt ccgtcgcccc ggacagggcg agccagggca
     8341 tgaccgtgtt gcggcccttc gacgcgaccg gggccgcgtc gcccgtcagc cccgcgaagg
     8401 agcggaagcg tccgatgaac tcctccttcg tcgcgcggcc gacgtacgcg tccttgtgcg
     8461 gcgtcaggtt cggcgtcacg ttgcggcccc acggacccgt gaacagcctt cccccggtgt
     8521 actcacgccc ggggatgggc gcgccggggc gcgtctcggg cgtgtgacag cccaggcaac
     8581 ccccgatcac ggtgaggtac ttcccgtatc cgatgtggtc cgtcttcggg tccggcgtga
     8641 cgaccggccc ggccaccggc ttgggcgcgc tcttgacgag aaggttcacc gggaagtcga
     8701 tcttcttcgc cggaaccgcg ttccggatcg gacgcagcgt ccggagatac aggacgatcg
     8761 cgcgcgcgtc ctcgtcgctc atctcgtggt acgcggcgta cggcatcatc gggaaaagcg
     8821 tgttgccgtc gcggtcgacg ccttcgcgaa tcgcgcgcag gatctcgccg tccgtccact
     8881 tcccgatccc cgtctccgga tcaggcgtga tgttgcgggc gtagaggacg ccggggatcc
     8941 cgaactcctt cgtgaaggcg aatccgccct tcccctcggt gccgggaatg cgcggcagcc
     9001 cgaaccggtc gaacttcgcg tcggagtggc acagcaggca gtccacgacg tgcatcgcga
     9061 ggtactcgcc gcgcgcgagg cgcgcgggcg tcgcctcgat cttctcggcg gacgcgggcc
     9121 gctgggcggg ctttttcgcg gcgagatacg cgatcccggc tcccacgatc acggcgagga
     9181 cgctcaggac gagaacgagt ccctttatca atcgcttcat gcgcgaagga tacgacgatc
     9241 cggcgccgtt tcagcgcccg agcggcaggc gcaggaagac gaggtcttcg accttctcgg
     9301 acgggagcac gccgtccggg ccgaaggcgt cgacctggct gttcgccacg tagacgtagt
     9361 cgtcgccgac cgccgtcccg cccacgggca cccggtacga cgggtgagcg cagtcgagga
     9421 tttccatccc cgtgaaggag ttcccgtcgg gttcgagcgt ccagcggccc acgcgcccca
     9481 cgctgacggt gccctggacg ccgacgagcg cgttgcgatg gaagacgagc ccgtcgaggc
     9541 cggccggcca cgggcccggc ggctggtcga ggagcgtcgc cgcccccgtc gcggcgtcta
     9601 ggcggtacac gccgcgcgcg aggtcgctga cccagacgcg cgcgccgtcc gccgagaacg
     9661 cgacggattt cggcgcgacg aacgcgccag ccgccgcggc gaccgcgagc tcctcggcgc
     9721 cggctttgag gacgtggatc tctccggacg ccgtgtccgt gacccagacg ttgccggctg
     9781 cgtccaccgc gagcccgccg aactgatgcg gcggtctcgt caggggcgac cagcggccga
     9841 ggatcgcgcc ggtcgcgagg tcgaacgcaa cgagctgcga gcgcccgtag tcctcgggac
     9901 ggaacccctc catcgccggg tcgccggccg tcacggccca caggcggcgg cgcgcggtgt
     9961 cgaccttcag gccgagaacg gcgtcgaggg ccgtgccgtc cgagcccgcg aagtcggcca
    10021 tcgcggccgg cgcgccggcg gcgcccggcg tcacgcgcac gatcttccgt tttcgcaggc
    10081 tcgccacgta gaagaccttc gcggcggcgt cccatgcgag gtcggcgggg acgaggtcgc
    10141 gcgccccgag cgtgaacgcg accgccgagc ggtgcgtctt cggcgcctgc gcgcggatca
    10201 cggcgagcgc gtcgttgaat cgcgggtcgg acgcgatgcc ctcgaacgac ttcggtcgcg
    10261 ggacgaggca gctgttgagc cgccatacct ggtccagcca ctcgagcgcg gccggggcgt
    10321 tgcccgcgtg cgcccaggcc gtcgccgcga ggtatgcggc cgcgtcgtcc ttcgtgttga
    10381 acgcgaatgc gcgctggaag ccgtcgcccg aggtttccca ctgccggcgc ccgaagtcga
    10441 ggtgacctcc cgcgtagccg ttcgcgatcg cggccgcctt ctgcgcgccg agcttcgagg
    10501 ccggcggggc cgccggcgcg gaggccgtcg aagcgccgcc gagcgagcaa cccgacaacg
    10561 agccgcgcga gctggcgacc g
//
LOCUS       DAOMFY010000123        10545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1932_length_10545_cov_8.566921, whole genome shotgun sequence.
ACCESSION   DAOMFY010000123 DAOMFY010000000
VERSION     DAOMFY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1932_length_10545_cov_8.566921"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1383)
                     /locus_tag="PLB01_11060"
     CDS             complement(64..1383)
                     /locus_tag="PLB01_11060"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQQ77886.1"
                     /translation="MSAASPRVAVIGGGLAGLVAADRLLGAGLPVTVFEKYPEAGGLV
                     GVVDVGGTALERYYHHLFTSDVDYVSFAEEFGLGGDLQWLRSRMGFFSGGTLWDFGTP
                     ASLLAFAPLGILGRLEFVLSTLRLRHSKDWRSLEGDTAVAWMRRNGYARALDAVWGPL
                     LFQKYGRRAEEVGLVWLWGKIALRTRSRDKTGLGERLGYLKGSFGRGVAAVLGRVKAR
                     GGDVRTSRPVKIVRKAGGGFDVEFAGGTETFDLVLSTVAIPELLRLAPDLPEDVRKTW
                     SAISYCHALCPILELDRSLSPYYWLNVGDASMPFGGVIEHTNFIPKEEYGGRHVVYIS
                     NYVLPDDPKWRKPDDALWAEYLPALKRINPAFDESWILKKQIGRAEYAQPIVVPGYSR
                     LLPPLETPVPGLWSACMAQIYPEDRGQNYAVRIGRDAADAILKTLRG"
     gene            1437..3521
                     /locus_tag="PLB01_11065"
     CDS             1437..3521
                     /locus_tag="PLB01_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HQQ77887.1"
                     /translation="MTALSAFLFASVLLLASASGMALLARLFRGDADGDAPAEAVFAF
                     SLPVGLVAAALPGWLVSGFLPATPIRSLALPLAGLALLALLLWGGRDLAGVLARGRAL
                     AAPLVLFLAIFAVFLWIRATYGEIRQTEKPMDFAVLSGLMATPRLPFMDPWLSGSRFP
                     YYHFGTYLFALPARAARVPSEYAYNLIAALLPAIAALAAYGVVRIRGGGRRLALLAGS
                     VLVLGGTFDGARQALGDRPLQDLDWWASSRRVANAITEWPLFTFRLGDLHPHAVTFPF
                     LLAFAGLAGKVGTAWGTLLDGVLLAAVLSANPWDLPACLLVLAAGNLAERPFRPAVIR
                     SVFSILLAVPFLLPFLRSPRPALHGLTFWKEATTAGEAFLHFGVLAVVPALAFGVALV
                     RSQSREDRALVAATAFPALGLALAVLTKRPVFGLAAAFAAGVLWLLFREPAADEAPAP
                     TGALRAGFLYAAAGAVLASVPDVVLVSDSYGDQMRRMNTVFKTFMGAWPLLVIGGALL
                     LPLALSTRRARGLIRAALLVTAGGLLAHPLAAAVLRWKWTGPIDGLNGLAWMEREAPG
                     DRAAIQWLRAHAVEGAVVAEATGNAYSDYSRIGGAAGLPIVLGWDNHEGLWRGAAAGP
                     ELEGRRRDLRLLYTSEEPDAVFATLHRYGVRYVVVGSFERRDFGVNAFPLRANFRRVF
                     ETKDTAVYEILK"
     gene            3518..5251
                     /locus_tag="PLB01_11070"
     CDS             3518..5251
                     /locus_tag="PLB01_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HQQ77888.1"
                     /translation="MTAATAPAAPPLSAFEKRAWIGLIALSLFLHLARLGTHAFHHDE
                     SIHAYASVRLLKEGAYKYDPVYHGPVQYFAVTAVFAVSSVVGKAVGFLTGFAAAKTWD
                     GTSDFTARLAPALGGVALTAMALLLRRRFGARAAFAAGALAALSPNILYYTRFVRDDV
                     WSLLGTAGLFLWLDEYLATRRVKDLAIAALWAAVAFASKENFYVLLALMVPSVAAAWL
                     EPGRGLDVWNRLRQLIDWLEENATAVLGALLLFFVTSEVLYTVFLVHPESGNPAFDAI
                     SYWWGQHKMERVGGPKTFYLPRLLQYEFAVLIPALAYVVARWKKLSFAERFVAGWAVS
                     SVAMYAYLGEKAGWLLVHQVLPFLPLAGAAWALLAEKGSGWRLAGAATAFASVATALT
                     LSFIYPDLTPNSRKAESTIQVQTCPEVLGIVDEILRSGRAGEVPAAIVSGEAGWPLSW
                     YVRPAPVNWQPPTGELHPPIVVCDDADADKQAQILGPGYTRDHVPFRAWWIPDTAISP
                     LRPSPGQLLRYLFTRDAWDRADGNNPIGAVYVTVFRRQYPKGAPPPALPPPPQEPPAA
                     EPKAAEPKATS"
     gene            5248..6192
                     /locus_tag="PLB01_11075"
     CDS             5248..6192
                     /locus_tag="PLB01_11075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ77889.1"
                     /translation="MTASAPEISVALPVFNEADNLDELRDRLVKALDAAGRTWEIVFV
                     DDGSRDGSFEKMAEFARADRRIRALRFSRNFGHQMALTAGVDAARGRVVAVMDADLQD
                     PPELLVDMLKKIDEGYEVVYAQRTKREGESAFKLWTAHVFYRLLKRWTNVDIPVDVGD
                     FRLMGPKAVAAFRRLRERHRFIRGMTAWIGFRQIGVPYVRPPRTRGETKFPLRKMLRF
                     ALDGLTSFSHVPLQLATWLGFLVSAFAFLYILVVLALWILKINVPGWTTLMVFVLLLG
                     GVQLMVIGVLGEYLGRIYDEVKGRPLYLVAEEVGGDES"
     gene            6237..7220
                     /locus_tag="PLB01_11080"
     CDS             6237..7220
                     /locus_tag="PLB01_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQQ77890.1"
                     /translation="MKSGYYHHAIGDLRARLSAAVPNEVLKALHRKTPWRHFAVALRQ
                     AVLFAGAFTLAWNFESLWVRIPCALVLGFVVFDGTVLLHEVVHKAVFDGDRPAAYRAL
                     GLLYALPSGISPTQFTRWHLDHHAELGSSTDDPKRAHLSPKINARWLKLLYATPALFP
                     IYFRAARKETATYPETLRRRIAGERLAAIALHLVAAAAIARFGGAARLVWLYLVPVFL
                     VFPPAFALNRLGQHYDIVPSQPAKWGTRMARSPLWEFAYLWSNLHLEHHYYPAVPFYR
                     LPALNTALVPFFEKEGIPARRYRDLLRGWIVENRAPHSDWSAPAAPATPPA"
     gene            7238..9373
                     /locus_tag="PLB01_11085"
     CDS             7238..9373
                     /locus_tag="PLB01_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HQQ77891.1"
                     /translation="MASEAAAPRVDKYEGLTRDELLRAFRNMVTSRRIDDREIQLKRQ
                     NRIFFQISGAGHEALQTAIAAHMKKGVDWLFPYYRDRALCLGLGVTPREMFLQAVGAA
                     LDPASAGRQMPSHWGSARLNVFTTSSPTGSECLPGVGAAEAGRYLLRPDAAAIRDQAS
                     HASPDEVVVVTIGEGATSEGEFWEALNSAATLKLPVVFVVEDNGYAISVPVEVNTPGG
                     SISRLVRSYPGLFVVEVDGCDYLASYDAFRYAFAYARERKGPALVHGHVVRPYSHSLS
                     DDEKLYRPENERQKDAERDPIVLFGKFLESEGLATADEIKALRDEVDAEVARAADEAL
                     ASPQPAAESVYENVYSPDVDPTSPAFDTEDAPAFSGEPTTMVDLVNAALRDEMARDGR
                     IAVWGEDVADASRADVLGEVKGKGGVFKATAGLQKAYGSARIFNAPLAEASIVGRALG
                     WAARGLKPVVEIQFFDYIWPAMQQLRDELATVRWRSGGAWKAPVVVRVAIGGYLTGGG
                     PYHSQSGEVTFAHIPGLRVVMPSSALDANGLLRTAIRCDDPVLFLEHKHLYRQTHNKG
                     RYPGRDFMIPFGKAKVVRPGHDVTVVTYGATVYRSTVAAAALAEEAGREAEVVDLRTL
                     APYDWDAIAASVKRTNRLLVVHEDWKTHGFGAEIAARAADELFEWLDAPVRRVAAKDV
                     FCGYAPQLEDATLPQSDDILDALRDLVAY"
     gene            9502..10455
                     /locus_tag="PLB01_11090"
     CDS             9502..10455
                     /locus_tag="PLB01_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ77892.1"
                     /translation="MSKRPALLRSGLAAIAFLFFFLFGALAVDPFPAYRAVMEEWLAV
                     SGGHREVFAADDGTRLSVWVLGPLSAERPVVLLHGLGADASYWAGTARFLVHAGRTVL
                     LPDGPGSGRSERPRDVSGYRIPGRVAALDALFRALALDKADLVGHSLGGWTVAAFALR
                     HPEKTGRLVLVDAGGFELPADIEAERRRVLPEDRPGARRVLDLLFFRPPFPAAGFVVN
                     AFGRNYGGDPAAATVHALDVTDGLEGRAKDLPPGTAVIWGAEETLFPLPVGKKLAAEI
                     PGGRLFVLPGAGHDGPLETPRAFRAALADALTSASQSGDSR"
BASE COUNT         1340 a         4023 c         3546 g         1636 t
ORIGIN      
        1 cgtccacggc ctcgtctcgc ccggcgcgcc gggccccgac ggccagctgc cgctctgggt
       61 cgactagccg cgcagcgtct tcaggatcgc gtccgccgcg tcgcgcccga tgcggacggc
      121 gtagttctgg ccgcggtcct cggggtagat ctgcgccatg cacgcggacc atagcccggg
      181 cacgggcgtc tcgagcggcg gcaggagcct cgagtagccc ggcacgacga tcggctgcgc
      241 gtactccgcg cggccgatct gcttcttcag gatccagctc tcgtcgaacg ccgggttgat
      301 gcgcttgagc gcgggcaggt actcggccca gagcgcgtcg tccggcttcc gccatttggg
      361 atcgtccgga aggacgtaat tggagatgta gacgacgtgc cggccgccgt actcctcttt
      421 cgggatgaag ttcgtgtgct cgatgacgcc gccgaacggc atcgacgcgt cgccgacgtt
      481 cagccagtag tacggcgaca gcgagcggtc gagttcgagg atcgggcaga gcgcatggca
      541 gtaggagatc gcgctccacg ttttccgcac gtcctcggga aggtccggcg cgagccgtag
      601 gagttcgggg atcgcgaccg tcgagaggac gaggtcgaac gtctccgtcc cgcccgcgaa
      661 ctccacgtcg aagccgccgc cggccttccg gacgatcttc acggggcgcg aggtgcggac
      721 gtccccgccc cgagccttca cgcgcccgag cacggccgcg acgccgcgcc cgaacgagcc
      781 cttcaggtac ccgaggcgtt cgccgagccc ggtcttgtcg cgcgagcgcg tgcggagcgc
      841 gatctttccc cagagccaca cgagcccgac ctcctcggcg cggcggccgt acttctggaa
      901 gagcagcggg ccccagacgg cgtcgagcgc acgcgcgtac ccgtttcggc gcatccacgc
      961 gaccgccgtg tcgccctcga ggctccgcca gtccttgctg tgccggagcc tgagggtcga
     1021 gaggacgaac tcgagccggc cgaggatccc gagcggcgcg aaggcgagga gcgacgcggg
     1081 cgtgccgaag tcccacagcg tcccgcccga gaagaacccc atccgcgacc tgagccactg
     1141 gaggtccccg ccgaggccga actcctcggc gaaggacacg tagtcgacgt cgctcgtgaa
     1201 gaggtggtgg tagtagcgct cgagagccgt gccgcccacg tccacgacgc cgacgaggcc
     1261 gcccgcctcc gggtacttct cgaaaacggt cacggggagg cccgccccga gcaggcggtc
     1321 cgccgcgacg agccccgcga ggcctccgcc gatcacggcg acacgtggag aggcggcgct
     1381 cacgggccca gtctagcccg gcgcggaccg cgctttgtgt taccttgatt cgttccgtga
     1441 cggccctgtc cgcgttcctc ttcgcctcgg tcctcctcct ggcgagcgcg tcgggaatgg
     1501 ccctgctcgc ccgcctcttc cggggcgacg cggacggcga cgcgcccgcg gaggccgtct
     1561 ttgcgttctc ccttcccgtc ggcctcgtgg cggccgccct gcccggctgg ctcgtctcgg
     1621 gtttcctgcc ggccacgccg atccgctcgc tcgcgctgcc gctcgcgggc ctcgcgctcc
     1681 tcgcgctcct cctctggggc gggcgcgacc tggcgggcgt cctcgcgcgc ggccgcgcgc
     1741 tcgcggcgcc gctcgtcctc ttcctcgcca tcttcgccgt cttcctctgg atccgggcga
     1801 cgtacggcga gatccgccag accgagaagc cgatggactt cgccgtcctg tcgggcctga
     1861 tggcgacgcc gcgcctgccg ttcatggacc cgtggctctc cggctcgcgc ttcccgtact
     1921 accacttcgg gacgtacctc ttcgcgctgc cggcgcgcgc ggcgcgcgtg ccgtccgagt
     1981 acgcgtacaa cctgatcgcc gcgctcctgc ccgcgatcgc cgccctcgcg gcttacgggg
     2041 tcgtgcggat ccgcgggggc gggcggcgcc tcgcgctcct cgcgggctcg gtcctcgtcc
     2101 tcggcgggac gttcgacggc gcgcggcagg ctctcggcga caggcctctc caggacctcg
     2161 actggtgggc gtcctcgcgg cgcgtcgcga acgcgatcac ggaatggccg ctcttcacct
     2221 tccggctcgg ggatctccac ccgcacgccg tcacgttccc gttcctcctc gcgttcgccg
     2281 gcctggccgg caaggtcgga acggcctggg gcacgctcct cgacggggtc ctcctcgcgg
     2341 ccgtcctctc ggcgaaccct tgggacctcc cggcgtgcct cctcgtcctc gccgcgggca
     2401 acctcgccga gcgtccgttc cgtcccgccg tcatccggag cgtcttctca atccttctcg
     2461 ccgtcccgtt cctcctgccc ttcctccgtt cgccgcggcc ggcgctgcac ggcctgacgt
     2521 tctggaagga ggcgacgacc gcgggagagg cgttcctcca cttcggcgtg ctcgccgtcg
     2581 tccccgcgct cgcgttcggc gtcgcgctcg tccgctcgca gtcgcgcgag gaccgcgcgc
     2641 tcgtcgccgc gacggcattc cccgcgctcg gcctcgcgct cgccgtcctg acgaagcgcc
     2701 ccgtgttcgg cctcgcggcg gcattcgccg cgggcgtcct gtggctgctc ttccgggagc
     2761 ccgccgcgga cgaggcgccc gctcccacgg gcgcgctccg ggccgggttc ctctacgcgg
     2821 ccgcaggcgc cgtcctcgcc tccgtgcccg acgtcgtcct cgtctcggac tcgtacggcg
     2881 accagatgcg ccgcatgaac acggtgttca agacgttcat gggcgcatgg cccctcctcg
     2941 tgatcggcgg ggccctcctc ctgccgctcg cgctctcgac gcgacgcgcg cgcggcctga
     3001 tccgcgccgc ccttctcgtc acggccggcg gcctcctcgc gcacccgctc gcggccgcgg
     3061 tcctccgctg gaaatggacg ggcccgatcg acgggctgaa cggcctcgcc tggatggagc
     3121 gcgaggcccc gggcgaccgc gcggccatcc agtggctgcg cgcccacgcc gtcgagggcg
     3181 ccgtcgtcgc ggaggcgacg ggcaacgcgt actcggacta cagccgcatc ggcggcgcgg
     3241 cggggctgcc gatcgtcctc ggctgggaca accacgaggg tctctggcgc ggcgccgccg
     3301 ccggccccga gctcgagggc cgccgacggg acctgcgcct cctctacacc tccgaggaac
     3361 cggacgccgt gttcgccacg ctccaccgct acggcgtgcg ttacgtcgtc gtcgggtcgt
     3421 tcgagcgccg ggacttcggc gtgaacgcct tcccgcttcg cgcgaacttc cggcgcgtct
     3481 tcgagacgaa ggacaccgcc gtttacgaga tcctgaaatg acggccgcga ccgctcccgc
     3541 cgctcccccg ctctcggcgt tcgagaaaag ggcctggatc ggcctgatcg ccctctcgct
     3601 gttcctgcat ctcgcccggc tcggaacgca cgcgttccac cacgacgagt cgatccacgc
     3661 gtacgcgtcg gtcaggctcc tcaaggaagg cgcgtacaaa tacgaccccg tctatcacgg
     3721 gcccgtccag tacttcgccg tcacggccgt gttcgcggtg tcgtcggtcg tcggcaaggc
     3781 cgtcggattc ctgaccgggt tcgccgcggc caagacctgg gacggaacgt ccgacttcac
     3841 cgcccgcctc gcgcccgcgc tcggaggcgt ggccctcacg gcgatggccc tcctcttgcg
     3901 ccggcggttc ggagcgcgcg ccgccttcgc ggccggggcg ctcgccgcgc tctcgccgaa
     3961 catcctctac tacacgcgct tcgtgaggga cgacgtctgg agcctcctcg ggaccgcggg
     4021 gctcttcctg tggctcgacg agtaccttgc gacgcggcgc gtgaaagacc tcgcgatcgc
     4081 ggcgctctgg gccgccgtcg ccttcgcgtc gaaggagaac ttctacgtcc tcctcgcgct
     4141 catggtcccg tccgtcgcgg ccgcgtggct ggagcccggg cgcggcctcg acgtctggaa
     4201 ccgcctgcgc cagctcatcg actggctcga ggagaacgcg acggccgtcc tgggcgcgct
     4261 cctcctcttc ttcgtcacgt ccgaggtcct ctacacggtc ttcctcgtcc acccggagtc
     4321 ggggaacccc gcgttcgacg cgatctccta ctggtggggc cagcacaaga tggagcgcgt
     4381 cggcgggccg aagacgttct acctcccgcg gctgctccag tacgaattcg ccgtcctgat
     4441 cccggcgctc gcgtacgtcg tcgcccgctg gaagaagctc tcgttcgccg agcgcttcgt
     4501 cgcgggctgg gccgtgtcct cggtcgcgat gtacgcgtat ctcggcgaga aggccggctg
     4561 gctcctcgtg caccaggtcc ttccgttcct cccgctcgcg ggcgcggcgt gggcgctcct
     4621 cgcggagaag gggtccggct ggcggctggc cggcgccgcg accgcattcg cttcggtggc
     4681 cacggcgctc acgctctcgt tcatctaccc ggacctgacc ccgaactccc gcaaggccga
     4741 gtccacgatc caggtccaga cgtgccccga ggtgctcgga atcgtcgacg agatcctccg
     4801 gtccggccgc gccggcgagg tgcccgcggc catcgtctcg ggcgaggccg gctggccgct
     4861 gagctggtac gtccggcccg cgccggtcaa ctggcaaccg ccgacgggcg agctgcaccc
     4921 gccgatcgtc gtgtgcgacg acgccgacgc cgacaagcaa gcgcagatcc tcggtcccgg
     4981 ctacacgcgc gatcacgtgc cgttccgcgc ctggtggatt cccgacaccg cgatctcgcc
     5041 gctccgcccg agcccgggcc agctcctgcg ctacctcttc acgcgcgacg cgtgggaccg
     5101 cgcggacggc aacaacccga tcggcgccgt ctacgtgacc gtgttccgcc ggcagtatcc
     5161 caagggggcg cctccgcccg cgcttcctcc tccgccgcag gagccgccgg ccgcggagcc
     5221 gaaggccgcc gagccgaagg ccacgtcgtg accgcgagcg cgcccgagat ctccgtcgcc
     5281 ctgcccgtct tcaacgaggc cgacaacctc gacgagctca gggaccggct cgtgaaggcg
     5341 ctcgacgccg cgggccggac atgggagatc gtcttcgtcg acgacggcag ccgggacggc
     5401 tcgttcgaga agatggccga gttcgcgcgg gccgaccgcc gcatccgcgc cctgcgtttc
     5461 tcccgcaact tcggccacca gatggcgctc acggcgggcg tcgacgcggc gcgcgggcgc
     5521 gtcgtcgccg tgatggacgc cgacctgcag gacccgcccg agctcctcgt cgacatgctg
     5581 aagaagatcg acgagggcta cgaggtcgtc tacgcgcagc gcacgaagcg cgagggcgag
     5641 agcgccttca agctctggac cgcgcacgtt ttctaccggc ttctcaagcg ctggacgaac
     5701 gtggacatcc ccgtcgacgt cggcgacttc cggctcatgg ggccgaaggc cgtcgccgcg
     5761 ttccggcggc tgcgcgagcg gcaccggttc atccgcggga tgacggcctg gatcgggttc
     5821 cgccagatcg gcgtcccgta cgtgcgcccc ccgcggacgc gcggcgagac gaaatttccg
     5881 ctgcgcaaga tgctgcgttt cgcgctggac ggcctcacgt ccttcagcca cgttccgctc
     5941 cagctcgcga cgtggctcgg cttcctcgtg tccgccttcg cgttcctcta catcctcgtc
     6001 gtcctcgcgc tctggatcct gaagatcaac gtccccggct ggacgacgct catggtcttc
     6061 gtcctcctcc tcggcggcgt ccagctcatg gtcatcggcg tcctcggcga gtacctcggc
     6121 cggatctacg acgaggtcaa gggccggccg ctctacctcg tggcggaaga ggtcgggggg
     6181 gacgagtcct gagacggaat ctccggcggg ggaattctgt tctaacctga gggcggatga
     6241 agtcgggcta ctaccaccac gcgatcgggg atctcagggc gcgcctctcg gccgcggtcc
     6301 cgaacgaggt cctgaaggcg ctgcaccgga agacgccgtg gcggcacttc gccgtcgcgc
     6361 tgcgccaggc cgttctcttc gcgggcgcgt tcacgctcgc gtggaacttc gagagcctct
     6421 gggtccggat cccgtgcgcg ctcgtcctcg ggttcgtcgt cttcgacggg acggtcctcc
     6481 tccacgaggt cgtccacaag gccgtcttcg acggggaccg ccccgccgcc taccgcgcgc
     6541 tcggcctcct ctacgcgctc ccgtccggga tctcgccgac gcagttcaca cgctggcacc
     6601 tcgaccatca cgccgagctc ggctcctcga cggacgaccc gaagcgcgcg cacctctcgc
     6661 cgaagatcaa cgcgcgctgg ctcaagctcc tctacgcgac gccggcgctc tttccgatct
     6721 acttccgggc cgcgcggaag gagaccgcga cgtatcccga gacgctccgc cgccgcattg
     6781 ccggcgagcg cctcgccgcg atcgcgctcc acctcgtggc ggccgccgcg atcgcccggt
     6841 tcggcggcgc cgcgcgcctc gtgtggctct acctcgtccc cgtcttcctc gtcttcccgc
     6901 cggccttcgc cctgaaccgg ctcggccagc actacgacat cgtcccctcc cagcccgcga
     6961 agtggggcac gcgcatggcg cgctcgccgc tctgggagtt cgcctacctg tggtcaaacc
     7021 tccacctcga gcaccactac taccccgccg tgccgttcta ccgcctgccc gcgctgaaca
     7081 ccgcgctcgt cccgttcttc gagaaggagg gcattcccgc gcgccggtac cgcgacctcc
     7141 tccggggctg gatcgtcgag aaccgcgcgc cccattcgga ctggagcgcg ccggccgccc
     7201 cggcgacgcc gcccgcgtaa ggtaaagtcc ggcagagatg gcttccgaag ccgccgcgcc
     7261 ccgggtggac aagtacgagg ggctgacgcg ggacgaactc ctccgcgcct tccgcaacat
     7321 ggtcacgtcc cggcggatcg acgaccggga gatccagctc aagcggcaga accgcatctt
     7381 cttccagatc tccggcgccg gccacgaggc tctccagacc gcgatcgcgg cgcacatgaa
     7441 gaagggcgtc gactggctct tcccgtacta ccgcgaccgc gcgctctgcc tcggactcgg
     7501 cgtgacgccc cgggagatgt tcctccaggc cgtcggcgcc gccctcgacc ctgcgtccgc
     7561 cggccgccag atgccgtcgc actggggctc ggcgcggctg aacgtcttca cgacgtcctc
     7621 cccgacgggc tccgagtgcc tgccgggcgt cggcgccgcc gaggccggcc ggtaccttct
     7681 ccgccccgac gccgccgcga tccgcgacca ggcgtcgcac gcgtcgccgg acgaggtcgt
     7741 cgtcgtcacg atcggcgagg gggcgacgtc ggagggcgag ttctgggaag cgctcaactc
     7801 cgcggccacg ctcaagcttc ccgtcgtctt cgtcgtcgag gacaacggct acgcgatctc
     7861 cgtccccgtc gaggtgaaca cccccggcgg ctcgatctcg cgcctcgtgc gctcgtaccc
     7921 gggactcttc gtcgtcgagg tggacggctg cgactacctc gcgtcgtacg acgcgttccg
     7981 ctacgcgttc gcgtacgccc gcgagcggaa gggccccgcg ctcgtccacg ggcacgtcgt
     8041 gaggccgtac tcgcactcgc tgtcggacga cgagaagctc taccgccccg agaacgagcg
     8101 ccagaaggac gccgagcgcg acccgatcgt cctcttcggg aagttcctcg agagcgaggg
     8161 cctcgcgacg gcggacgaga tcaaggccct ccgcgacgag gtggacgccg aggtcgcccg
     8221 cgccgcggac gaggcgctcg cgagccctca gccggcggcg gaatccgtct acgagaacgt
     8281 gtactccccc gacgtcgacc cgacgtcgcc cgcgttcgac acggaagacg ccccggcgtt
     8341 ttccggcgag ccgacgacga tggtcgacct cgtgaacgcg gcgctccggg acgagatggc
     8401 gcgcgacggg cggatcgcgg tgtggggcga agacgtcgcg gacgcgagcc gcgcggacgt
     8461 cctcggagag gtcaagggca agggcggtgt cttcaaggcc acggcgggcc tccagaaggc
     8521 ctacgggagc gcgcgcatct tcaacgcgcc cctcgccgag gcgagcatcg tcgggcgcgc
     8581 tctcggctgg gcggcgcgag gcctcaagcc cgtcgtcgag atccagttct tcgactacat
     8641 ctggcccgcg atgcagcagc tgcgcgacga gctcgcgacg gtgcgctggc gctcgggcgg
     8701 cgcctggaag gcgcccgtcg tcgtgcgggt cgcgatcggc ggctacctca ccggcggcgg
     8761 gccgtaccac tcgcagtccg gcgaggtgac gttcgcgcac atccccggcc tgagggtcgt
     8821 gatgccgtcg agcgcgctcg acgccaacgg cctcctgcgc accgcgatcc gctgcgacga
     8881 ccccgtcctg ttcctcgagc acaagcacct ctaccgccag acgcacaaca agggccgcta
     8941 cccgggccgc gacttcatga tcccgttcgg gaaggcgaag gtcgtccgcc ccggccatga
     9001 cgtgacggtc gtgacgtacg gggccacggt ctaccgcagc acggtcgccg ccgcggcgct
     9061 cgccgaggag gcgggccgcg aggccgaggt ggtcgacctc cggacgctcg cgccgtacga
     9121 ctgggacgcg atcgcggcga gcgtgaagag gacgaaccgg ctcctcgtcg tccacgaaga
     9181 ctggaagacg cacggtttcg gcgccgagat cgcggcgcgc gcggccgacg agctcttcga
     9241 gtggctggac gccccggtgc gccgcgtcgc ggcgaaggac gtcttctgcg gctacgcgcc
     9301 gcagctcgag gacgcgacgc tcccgcagag cgacgacatc ctcgacgccc tccgcgacct
     9361 cgtcgcgtat tgagaagaaa gagaagagaa gattttcttt gaagggtgtg ggtgctcacc
     9421 gcgctcgagg ggcgtcgggg ccagacccgg cgcggggacg ccaccagacc ttctaggaaa
     9481 tcctgatagc gtgcccaggt gatgagcaag cggcctgcgc tcctgcgctc cggtctggca
     9541 gcaatagcat ttctcttctt cttcctcttc ggcgcgctcg ccgtcgatcc gttcccggcc
     9601 taccgcgccg tcatggagga gtggctcgcg gtctccggcg ggcaccgcga ggtcttcgcg
     9661 gccgacgacg gcacccgcct gtccgtctgg gtgctcgggc cgctctcggc cgagcggccc
     9721 gtcgtcctcc tgcacggcct cggcgccgac gcgtcgtact gggccggcac ggcgcgcttc
     9781 ctcgtccacg ccgggcgcac ggtcctcctt ccggacgggc ccggctccgg ccggagcgag
     9841 cggccgcgcg acgtgtccgg ctaccggatc ccgggccgcg tggccgcgct cgacgcgctc
     9901 ttccgcgcgc tcgcgctcga caaggccgat ctcgtcggac attcgctcgg cggctggacg
     9961 gtcgcggcct tcgcgctccg gcacccggaa aagaccggcc ggctcgtcct cgtcgacgcc
    10021 ggcggcttcg aactgcccgc cgacatcgaa gccgagcgcc gccgcgtcct ccccgaggat
    10081 cgccccggcg cgcgccgcgt cctcgacctt ctcttcttcc ggccgccgtt tcccgccgcg
    10141 ggtttcgtcg tgaacgcgtt cggccgaaac tacggcggcg atcccgcggc cgcaacggtc
    10201 cacgcgctgg acgtgaccga cggactcgag ggccgcgcga aggatcttcc tccggggacc
    10261 gccgtgatct ggggcgcgga ggagacgctc tttcccctcc cggtcgggaa gaagcttgcc
    10321 gccgagattc ccggcggccg cctgttcgtc ctgccgggcg cggggcacga cggcccgctc
    10381 gagacgccgc gcgccttccg cgcggccctt gcggacgcgc tgacgtccgc ctctcagtcc
    10441 ggcgacagcc ggtagaccgt gaacgtgggc cgcccccagg ggtcgcgccc ccgcgcggcg
    10501 acggcgcgcg gcacgacggt gcgccgcacg agcggcaccg gcaac
//
LOCUS       DAOMFY010000124        10538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1933_length_10538_cov_8.311647, whole genome shotgun sequence.
ACCESSION   DAOMFY010000124 DAOMFY010000000
VERSION     DAOMFY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1933_length_10538_cov_8.311647"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="PLB01_11095"
     CDS             complement(<1..1763)
                     /locus_tag="PLB01_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HQQ77893.1"
                     /translation="MNLTVIDWAIVFAYFALSIGIGFAFTKKGGESLEEYFVAGRKVP
                     WWLAGAAMIATTFAADTPLVVTGLVAAHGVAGNWIWWNFVMSGTLTVFLYARLWRRAK
                     VMTDVEFAEIRYSGKPATFLRGFRALYLALPINLIILGWVTLAMVKILQISLGIRPLW
                     AVGLCFVITVGYSAAAGMWAVLWTDLVQLVIKMTAVILLAYFAVKKVGGIVALEEGLA
                     KHFGSVDAAISFLPPAGSAWMPAIALFTFLGVQWWAAWYPGAEPGGGGYVAQRIFSAK
                     TERDGILATLFFNVGHYALRPWPWILTGLATVLLYPAGVLVNGNPDHEAAYVQAMVDL
                     MPAGWKGFMLAGFAAAYMSTVGTHLNWGASYLVGDVYKRFLRRGASEKHYVAVSRWTT
                     VLLFLLSIVVTLNLSSIEGAWRFLLALGSGTGLVLILRWYWWRVNAWSEISAMVTSMA
                     VSLAAFAFVPKGTGPGGEFEQQAIVMLVTVVATTLVWLAVTFLTAPEPDATLDAFYTR
                     VRPGGPGWARVSARLGFGREPIPGGAMSFLNWGLGILLVYTALFGIGKIVFGRILTGF
                     ELLDVAVVAFVLIMWNLKREE"
     gene            1850..5905
                     /locus_tag="PLB01_11100"
     CDS             1850..5905
                     /locus_tag="PLB01_11100"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQQ77894.1"
                     /translation="MRRVPLAVPLAMLFLFPGVRAAAGDESAKGEFAERLERARKAEE
                     RFREMHGAARSEVFRRAAVIGSAERRAELERARRSPLAAGPTWVSLGPADAPQGNFNG
                     AFPGLTDSGLINAIAIHPSEPRTIFVGPAGGGVWRSRDAGATWAPLTDDIGPTPIGAI
                     AIAPSDPKRIYAGGGSGRQSLGNDVPGPGLLVSTDGGDTWTLSASSPGGEFWFIRVDP
                     ANPRVLLAGTDKGLLRSTDGGDTWTKTLAAAGDAWANALAVSPANPSLLLAATFRRGV
                     ESFQGGACVVPASSTYRPGRLWKSTDGGATWDENVNLPGSGPPVKRSRVELAFAPSDP
                     AVVYALIAKGSVDANGCSDDLTYGDLFDVAKSTDGGDSWSALGAADALKDLPNGQMYY
                     AAALSVDPRDARTVWVGGLDNYKSTDGGATWKRVSAWTNGRPAAPYVHADQHFHLWAP
                     DGSTLYLGCDGGLFSTTDGGASYASLNRGLVTFQFFSVCQSPAAPGVVMGGAQDNGTS
                     LRKADGSWTQVIGGDGFGCLLHPTNPQILLGSVQNERFYRSTDGGNLFGGVSITPGTG
                     DTSTFLTRLVQDPASPEHVLTSTTLRLWESLDGGGTWKGLGTGTIPGIASVRDFALSP
                     IDSKIVGVAANSGKVLLSSDGGATFSPAGTLPVNTLSSIRFDRTNPRNLWVASALATA
                     GVERVFASTDGGATWSAISRTGKPNGLPDLPVAQMEQDPLDPNVLWAVSYVGVYRSPD
                     RGGTWARYGTGLPNVPLSAISILPDGSKMRLATFGRAIWEISLVALPPDQPPSAAIDV
                     PAATPTLDAGLPASFHGVATDPDAGDVLTLTWDFGDGRTAAGASASHAFAVPGTYTVK
                     FRATDAAGLFAEATRTVNVRIPQGSVATGPELFLPVVLDVVGAGGAHYTTELTLISRA
                     ASPTTVLLQYTGSAGGGSGYASVTLQPGEVRVVPDAISFLTSAGLPLPADGTRIGTLR
                     AIYPGAAADEVFIGGRTSTPGAGGTFGLFYTGASASATTATVFGLQQSAAQRSNLALV
                     NAGPDPVTLRVALQGPNGEALTGPSDQTLPGWGWTQINQPLLGQADSGRAVVTKISGA
                     GPFTAYGVLNDATTSDGSFVPALVPGDVSGADRLVPIVLAAAGYRSELTLTNFTTEPM
                     PLTLTYTGSPQLSASGSGSAALTLQPGEQRILPDSMAFLRSLGLQIPSSGNVGGALRV
                     KAPNGTPASSLAAGARTFTNAAGGGTFGLFYPGLTHGESATALAYINGLQQNAVQRSN
                     LAVVNRGDAADAITLKVTYFGADGAALADPDTATLAPGEWRQFNQPLGARGALAGFAR
                     IERLAGASRWVAYGILNDQHNSDGSYIPMSR"
     gene            5969..6796
                     /locus_tag="PLB01_11105"
     CDS             5969..6796
                     /locus_tag="PLB01_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HQQ77895.1"
                     /translation="MKDLSEVLAAEGEIVRLDRDHPGFKDPVYRARRNEIALHALRYK
                     AGEPIPRVDYTPEEQAVWRLTWERLGPLHETYACREWIESSKVLSLDRTAVPQLADVN
                     PTLARSGFRMLPVAGLIQGRGFLSAMAQGVFLSTQYMRHHSVPFYTPEPDVIHELVGH
                     AASLFHPDIVRLSRLFGEAAARASDEGMKRLELVYWYTLEFGLVEEGGALRTYGAGVL
                     SSFGEMERMTTEAELRPLDFADASTRPYDPTQYQPVLYVSPSWDRMVADVSDWLKTV"
     gene            6820..8118
                     /locus_tag="PLB01_11110"
     CDS             6820..8118
                     /locus_tag="PLB01_11110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ77896.1"
                     /translation="MPPPPRPYDALKHRDFRRLAITQLLSLVGSQMQNVAIDWHVWVL
                     TRSPLALGLVGLVRVVPIVTLSLVGGLVADRHDRRRVMLATQSAMTLVALALGAATLL
                     GRDTVGLVYALTAATSAAGAFDNPARQSLVPRLVPERDLPGALAILLSAFQVAAIGGP
                     ALTGLILAGSAHASPAAGSAHPHTAGLALVYLLNAASFAGVLATLATLRASGRVERAE
                     GAPAHPIESLREGLRFVFTTPILVWTMTLDFFATFFAGSMSLLPIFADQILLVGPAGY
                     GWLRAAPGIGALASSVWTSVRGLPRRQGRVFLWAVAAYGAVTIVFGLSRSFALTFAAL
                     ALVGLTDTISTVVRQTVRQLVTPDALRGRMTGVNMIFFMGGPQLGELEAGFVASLFAS
                     AAMGASFAVVSGGVGTILVAAFVAWRARDVRDYEWRAPGG"
     gene            8189..>10538
                     /locus_tag="PLB01_11115"
     CDS             8189..>10538
                     /locus_tag="PLB01_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQQ77897.1"
                     /translation="MRLSNVCAAALLVAMVAVAAPEEQPKSPSKKSLSSSSSSSSSSS
                     SSSSEAEKSLSDKLQGLQFRNIGPFRGGRVTAVAGVPGQPLTFYFGGTGGGVWKTTDG
                     GSSWEPVSDKFFRTGSVGALAVAPSDPNVVYAGMGEGCIRGNTSAGDGVWKSVDSGKT
                     WTNVGLGDTQQIPRVRVHPSNPDLVYVAALGHTWGPNPDRGIFRSADGGKTWKKVLFV
                     DEKTGASDLVMDATNPRILWAGFWQVGRKPWSLESGGPGGGVWKTTDGGDTWKKLSGG
                     LPEGVVGRVGIAVSPARPERAWAVVEAAKGGVFRTDDGGETWKKVNDENKLRQRAWYY
                     SHVVADPKNADAVYVLNVGFFRSGDGGKTFDAIRTPHGDNHDLWIDPDDPQRMIEGND
                     GGAAVTFNGGRSWSSVLNQPTAQFYRVVADARFPYRVYGAQQDNTTVEILSRTRGGGI
                     GPSDWHPVGGCESGWIAPKPDDPDVSFAGCYGGAIGRFDGRTGEERNVVAWPQLAIGQ
                     APKDLKYRFQWNAPILISRHDSRTLYHASQVLLRSTDEGATWTEVSPDLTRNEKATQG
                     YSGGPITFDNTGVEVYGTIFALAESPAEAGTIWAGTDDGLVQLTRDGGKNWANVTPKG
                     LPERIQVNSIEVSPHDAGAAWIAATMYKHDDARPYLYRTSDYGKTWTKLVNGIPDGAF
                     TRAVREDPVRKGLVFAGTERGLYVSLDGGAAWQPFSRNLPVVPITDLTIKDGDLVVAT
                     QGRAFWILDDLSPLRQWADGVEKKAVHVFKPRPAVRFGSAGRG"
BASE COUNT         1562 a         3739 c         3691 g         1546 t
ORIGIN      
        1 ttctcttccc gcttcaggtt ccacatgatg agcacgaagg cgacgacagc gacgtcgagg
       61 agctcgaagc cggtgaggat ccgtccgaac acgatcttgc cgatcccgaa cagggccgtg
      121 tacacgagga ggatcccgag gccccagttc aggaaggaca tcgcgccgcc cgggatcggc
      181 tcgcggccga agccgagccg tgccgagacg cgcgcccagc ccggcccgcc gggccgcacg
      241 cgtgtgtaga aggcgtcgag cgtcgcgtcg ggctcgggcg cggtcaggaa cgtcacggcc
      301 agccagacca gcgtcgtcgc gacgaccgtc acgagcatca cgatcgcctg ctgctcgaac
      361 tccccgcccg gccccgtccc cttcgggacg aacgcgaagg ccgcgagcga gacggccatg
      421 gacgtcacca tcgcggagat ctcgctccac gcgttgacgc gccaccagta ccagcggagg
      481 atcaggacga gacccgtccc gctcccgagc gcgaggagga accgccaggc accttcgatg
      541 gacgagaggt tgagcgtcac gacgatggac agcaggaaca gcaagaccgt cgtccaacgc
      601 gagacagcga cgtagtgctt ctcgctcgca ccccttctca ggaatctctt gtagacgtcc
      661 ccgaccagat acgaagcccc ccaattcaga tgtgtcccca cggtcgacat gtacgcggcc
      721 gcgaagccgg cgagcatgaa gcccttccag ccggccggca tgaggtcgac catcgcctgg
      781 acgtacgccg cttcgtggtc cggattgccg ttcacgagga cgccggcggg gtagaggagc
      841 accgtcgcga ggcccgtgag gatccacggc cacggccgga gcgcgtaatg gccgacgttg
      901 aagaacagcg tcgcgaggat gccgtcgcgc tcggtctttg ccgagaaaat gcgctgcgcg
      961 acgtagccgc cgccgccggg ctcggcgccg gggtaccacg cggcccacca ctgcacgccg
     1021 aggaacgtga agagtgcgat cgcgggcatc cacgccgacc ccgcgggcgg gaggaaggag
     1081 atcgcggcgt cgacggagcc gaagtgcttc gcgagccctt cctcgagcgc cacgattccg
     1141 ccgaccttct tcaccgcgaa atacgcgagg aggatgacgg cggtcatctt gatgacgagc
     1201 tggacgaggt cggtccagag aacggcccac attccggccg ccgctgagta gccgaccgtg
     1261 atcacgaagc agaggccgac ggcccagagc ggacggatcc ccagcgagat ctggaggatc
     1321 ttgaccatcg cgagcgtgac ccagccgagg atgatgagat tgatcgggag cgcgagatag
     1381 agcgcgcgga agccgcgcag gaacgtcgcg ggcttcccgc tgtagcggat ctctgcgaac
     1441 tcgacgtccg tcatgacctt cgcgcggcgc cagaggcgcg cgtagaggaa caccgtgagc
     1501 gtcccggaca tcacgaagtt ccaccagatc cagttgcccg cgacgccgtg cgcggcgacg
     1561 aggcccgtca cgacgagcgg cgtgtcggcc gcgaacgtcg tcgcgatcat cgcggcgccc
     1621 gcgagccacc acggcacctt gcggccggcg acgaagtact cctcgagcga ctcgccgccc
     1681 ttctttgtga aggcgaagcc gatcccgatc gagagggcga agtacgcgaa gacgatggcc
     1741 cagtcgatta cggtgaggtt catgccgcgg gcgtccgatt catgcgtgac ctccgaggat
     1801 tgaccgcgat gctacgacag ggagagactg agtcgcctct ggaggttcca tgcgccgcgt
     1861 ccctctcgcc gtcccgctgg ccatgctgtt cctgttcccc ggcgtgcgcg ccgcggcggg
     1921 cgacgagagc gcgaagggcg agttcgccga gcgcctcgag cgcgcgcgga aggccgagga
     1981 gcgcttccgg gaaatgcacg gcgcggcgcg cagcgaggtc ttccgccggg ccgccgtgat
     2041 cggatcggcc gagaggcggg ccgagctcga gcgcgcgcgg cggtcgcctc ttgcagccgg
     2101 ccccacgtgg gtgtcgctcg ggcccgccga cgcgcctcaa gggaacttca acggagcgtt
     2161 ccccgggctc acggactcgg gcctgatcaa cgccatcgcg atccatccgt ccgagccccg
     2221 gacgatcttc gtcggtcccg cgggcggcgg cgtctggcgg tcccgggatg cgggcgcgac
     2281 gtgggcgccg ctcacggacg acatcgggcc gacgccgatc ggggcgatcg cgatcgcccc
     2341 gtccgacccg aagcggatct acgcgggagg cggctcgggc cgtcagtctc tcggcaacga
     2401 cgtgccggga ccgggtcttc tcgtttcaac ggacgggggc gacacgtgga cgctctccgc
     2461 gtcctctccg ggcggcgagt tctggttcat ccgcgtggat ccggcgaacc cgcgcgtcct
     2521 cctcgccggg accgacaagg ggctcctgcg ttccacggac gggggcgaca cgtggacgaa
     2581 gacgctggcc gccgcgggcg acgcatgggc gaacgccctc gccgtgtcgc ccgcgaaccc
     2641 gtccctcctg ctcgccgcga cgttccggcg gggcgtcgaa tcctttcagg gcggggcctg
     2701 tgtcgtgccc gcgagctcga cgtaccggcc gggccgtctc tggaagtcga cggacggcgg
     2761 cgcgacgtgg gacgaaaacg tcaacctgcc cggctccggg ccgccggtca aacggtcgcg
     2821 cgtcgagctc gcgttcgcgc cctcggaccc ggcagtcgtg tacgcgttga tcgcgaaggg
     2881 ctccgtcgac gcgaacggct gctccgacga cctgacgtac ggcgacctct tcgacgtcgc
     2941 gaagtccacc gacgggggag actcgtggtc tgcgctcggc gcagccgacg ccctcaagga
     3001 cctcccgaac gggcagatgt actacgcggc cgccctcagc gtcgacccga gggacgcccg
     3061 gacggtgtgg gtcggcggcc tcgacaacta caagtcgacc gacggaggcg ccacgtggaa
     3121 acgcgtctcg gcctggacca acggaaggcc ggccgcgcca tacgtccacg cggaccagca
     3181 tttccatctc tgggctccgg acgggtcgac gctctatctc ggctgcgacg gggggctgtt
     3241 ctcgacgacg gacggcggcg cttcgtacgc gagcctgaac cggggactcg tgacgttcca
     3301 gttcttttcg gtctgccaga gccccgcggc gcccggcgtc gtgatgggcg gcgctcagga
     3361 caacggcacg agcctgcgca aggccgacgg gtcgtggacg caggtcatcg ggggagacgg
     3421 gtttggatgc ctcctccatc cgacgaaccc ccagatcctc ctcggcagcg tgcagaacga
     3481 gaggttctat cgctcgaccg acggcgggaa tctcttcggc ggagtcagca tcacgcccgg
     3541 gaccggcgac acttcgacct tcttgacgag gctcgtccag gatcccgcct ctcccgagca
     3601 cgtcctgacg tcgacgacgc ttcgcctgtg ggagtcgctc gacggcgggg gaacctggaa
     3661 gggcctgggg acgggcacga tccccgggat cgcgtccgtg cgcgacttcg cgctcagccc
     3721 gatcgactcg aagatcgtcg gcgtcgccgc gaattctggg aaggtcctcc tgtcctcgga
     3781 cggcggcgcc acgttttccc cggcggggac gctgcccgtg aacacgctct cgtcgatccg
     3841 gttcgaccgg acgaacccgc gcaatctctg ggtggccagc gcgctcgcga cggcgggggt
     3901 cgagcgcgtg tttgcatcta cggacggcgg cgcgacatgg agcgcgatct cgcgcactgg
     3961 caagcccaac ggcctcccgg accttccggt cgcgcagatg gagcaggatc cgctcgaccc
     4021 gaacgtcctc tgggccgtgt cgtacgtcgg cgtctaccgg tcgcccgacc gcggcggaac
     4081 ctgggcgcgc tacgggacgg gcctcccgaa cgttccgctc tcggcgatct cgatcctgcc
     4141 ggacgggtcg aagatgcgcc tcgcgacttt cgggcgcgcc atctgggaga tctcgctggt
     4201 ggcgctgccg ccggaccagc cgccgtcggc ggcgatcgac gtgccggccg cgacgccgac
     4261 gctcgacgcg ggtctcccgg cgagcttcca cggcgtcgcg acggatcccg acgccggcga
     4321 cgtcctgacc ctgacgtggg acttcggcga cgggaggacg gcggcgggcg cgtccgcgtc
     4381 ccacgcgttc gcggttcccg ggacgtacac ggtgaaattc cgcgcgacgg acgcggccgg
     4441 cctcttcgcc gaagcgacgc gcaccgtgaa cgtccgcatc ccccagggca gcgtcgcgac
     4501 cgggcccgag ctcttcctcc ccgtcgtcct cgacgtcgtc ggcgcgggcg gggcccacta
     4561 cacgaccgag ctcacgctca tctcccgggc cgcgtcgccc acgacggtcc tcctgcagta
     4621 cacggggtct gcgggcgggg gctcgggtta cgcgagcgtc accctccagc cgggcgaggt
     4681 ccgggtcgtc cccgacgcga tctccttcct gacgtcggcg ggactgcctc tcccggcgga
     4741 cgggacgcgg atcggaacgc tgcgcgcgat ctatccgggg gcggcggcgg acgaggtctt
     4801 catcggcggc cggacgtcca cgccgggcgc cggcggcacg ttcggcctct tctataccgg
     4861 ggcgtcggcg tccgcgacga cggccacggt cttcggcctc cagcagagcg cggcgcagcg
     4921 ctcgaacctc gcgctcgtga acgcaggacc cgatcccgtc accctgcgcg tcgcgctcca
     4981 gggcccgaac ggcgaggccc tgacggggcc ctccgaccag acgttgccgg gctggggctg
     5041 gacgcagatc aaccagccgc tcctcgggca ggcggactcg ggccgcgccg tcgtcacgaa
     5101 aatctccgga gccggcccgt tcacggcgta cggcgtcctg aacgacgcga cgacatcgga
     5161 cggcagcttc gttccggcgc tcgtgcccgg cgacgtctcc ggcgccgacc gcctcgtccc
     5221 gatcgtcctg gcggccgcgg gctaccggag cgagctgacg ctgacgaatt tcacgaccga
     5281 gccgatgccc ctcacgctca cgtacacggg ttcgccgcag ctctcggcgt ccggctcggg
     5341 ctccgcggcg ctgacgctcc agcccggcga gcagaggatc ctgcccgact ccatggcttt
     5401 tctgagaagt ctcggtcttc agattccttc ctccggcaac gtgggcggag cgctgcgggt
     5461 caaggcgccg aacggaacgc cggcctcctc tctggcggcg ggcgcgcgga cgttcacgaa
     5521 cgcggcgggc ggcggcacgt tcggcctctt ctacccgggc ctcacgcacg gcgagagcgc
     5581 gaccgcgctc gcgtacatca acggcctgca gcagaacgcc gtccagcggt cgaacctcgc
     5641 ggtcgtcaac cggggcgacg cggcggacgc gatcacgctg aaggtcacgt acttcggggc
     5701 ggacggcgcg gccctcgccg accccgacac cgcgacgctc gcgccggggg agtggaggca
     5761 gttcaaccag ccgctcggcg cgcgcggcgc gttggccggg ttcgcgagga tcgagcggct
     5821 cgcgggcgcg tcccggtggg tcgcctacgg catcctgaac gaccagcaca actccgacgg
     5881 gtcctacatc ccgatgagcc ggtagcccga gactccgtcg cgaaggatgg gttttcgcat
     5941 tacgatggcc ggcgatgagc gcccggccgt gaaagacctc tcggaagtcc tcgccgcgga
     6001 aggcgagatc gtccgcctcg accgcgacca cccgggattc aaggaccccg tgtaccgggc
     6061 gcgacgcaac gagatcgcgc tccacgcgct gcgctacaag gcgggagagc cgattccgcg
     6121 cgtcgactac acacccgagg agcaggcggt ctggcgtctc acctgggaac ggctggggcc
     6181 cctccacgag acgtacgcgt gccgcgagtg gatcgagagc tcgaaggtgc tctccctcga
     6241 ccggacggcc gtcccccagc tcgcggacgt gaaccccacg ctcgcgcgct cgggcttccg
     6301 catgcttccc gtcgcgggcc tgatccaggg ccgcggcttt ctctcggcga tggcgcaggg
     6361 cgtgttcctg tcgacgcagt acatgcgcca ccactccgtg cccttctaca cgccggagcc
     6421 ggacgtcatc cacgagctcg tcggccacgc cgcgagcctg ttccaccccg acatcgtccg
     6481 gctgtcacgc ctcttcggcg aagcggccgc gcgcgcgagc gacgagggaa tgaaacgcct
     6541 cgagctcgtc tactggtaca cgctcgagtt cgggctcgtc gaagagggcg gcgccctccg
     6601 gacctacgga gcgggcgtcc tctcgtcgtt cggggagatg gagcgcatga cgacggaagc
     6661 ggagctgcgc ccgctggact tcgcggacgc gtcgacgcgg ccgtacgacc cgacgcagta
     6721 ccagcccgtc ctctacgtct cgccgtcgtg ggaccggatg gtcgcggacg tctcggactg
     6781 gctgaagacg gtctgacccg ccgcccgtag actcccttcg tgcccccgcc tccgcgcccg
     6841 tacgacgcgc tgaagcaccg cgacttccgc cggctcgcga tcacgcagct cctctcgctc
     6901 gtcgggtcgc agatgcagaa cgtcgcgatc gactggcacg tctgggtgct cacgcgctcg
     6961 ccgctcgcgc tcggcctcgt gggcctcgtg cgcgtcgtcc ccatcgtgac gctctcgctc
     7021 gtcggcggcc tcgtcgccga ccgccacgac cgccgccgcg tgatgctcgc gacgcagtcc
     7081 gcgatgacgc tcgtcgccct ggccctcggc gcggcgacgc tcctcggccg ggacaccgtc
     7141 ggactcgtct acgccctgac ggcggcgacg tccgcggcgg gcgctttcga caacccggct
     7201 cgccagtcgc tcgtcccgcg cctcgtcccc gagcgcgacc ttcccggcgc gctcgcgatc
     7261 ctcctctcgg ccttccaggt cgcggcgatc ggcgggcccg ccctcacggg cctgatcctc
     7321 gcgggctcgg cgcacgcgtc gcccgccgcg ggaagcgccc acccgcacac ggcgggcctc
     7381 gcgctcgtct acctcctgaa cgcggcgtcc ttcgccgggg tgctcgccac gctcgcgacg
     7441 ctccgggcct ccggccgcgt cgagcgcgcg gaaggcgcgc cggcgcaccc gatcgagtcc
     7501 ctgcgggagg gcctgcgctt cgtgttcacg acgccgatcc tcgtctggac gatgacgctc
     7561 gacttcttcg cgacgttctt cgcgggctcg atgtccctgc ttcccatctt cgcggaccag
     7621 atcctcctcg tcgggccggc gggctacggc tggctgcgcg ccgctcccgg catcggggcg
     7681 ctcgcctcgt ccgtgtggac gtcggtccgc ggcctgccgc ggcggcaggg gcgcgtgttc
     7741 ctgtgggcgg tggccgcgta cggcgcggtc acgatcgtgt tcggcttgtc gcgaagcttc
     7801 gcgctcacgt tcgcggcgct cgcgctcgtc gggctgacgg acacgatctc gacggtcgtg
     7861 cggcagaccg tgaggcagct cgtgacgccc gacgcgctcc gcggccggat gacgggcgtc
     7921 aacatgatct tcttcatggg cgggcctcag ctcggcgagc ttgaggcggg cttcgtcgcg
     7981 agccttttcg cgtcggcggc aatgggcgcc tcgttcgccg tcgtctccgg cggcgtcggc
     8041 acgatcctcg tcgcggcgtt cgtcgcgtgg cgcgcgcggg acgtgcgcga ctacgagtgg
     8101 cgcgcgcccg gcggctgacg actgatatgg tttgagaaag agaagagaag aaccggggat
     8161 tcggattttc tttgaagggg gaagaggcat gaggctctcg aatgtgtgcg cggcggcgct
     8221 tctggttgcc atggtggcgg tggcggcgcc cgaggaacag cccaaatcac cttctaagaa
     8281 atctttgtct tcctcttcct cttcctcttc ctcttcttct tcttcttctt ccgaagcaga
     8341 gaaatctctt tccgacaagc tccagggcct tcagttccgc aacatcggcc cgttccgcgg
     8401 cgggcgcgtc acggccgtgg cgggcgtgcc cggacagccg ctcacgttct acttcggcgg
     8461 cacgggcggc ggcgtgtgga agacgacgga cggcggctcg agctgggagc ccgtctccga
     8521 caagttcttc agaacgggct ccgtcggcgc gctcgcggtg gcgccgtccg acccgaacgt
     8581 ggtgtacgcc ggaatgggcg agggctgcat ccgcgggaac acctcggcag gcgacggcgt
     8641 gtggaaatcc gtcgactccg gaaagacgtg gacgaacgtg gggttgggcg acacgcagca
     8701 gatcccgcgc gtgcgcgtcc atccgtcgaa tccggacctc gtctacgtgg cggccctcgg
     8761 acacacgtgg gggccgaacc cggaccgcgg catcttccgg tcggcggacg gcgggaagac
     8821 gtggaagaag gtcctctttg tcgacgagaa gaccggagcg tccgacctcg tcatggacgc
     8881 gacgaacccg cgcatcctct gggccggctt ctggcaggtg gggcgcaagc cgtggtcgct
     8941 cgagagcggc ggcccgggcg gcggcgtgtg gaagaccacg gacgggggag acacgtggaa
     9001 gaagctctcg ggcggcctgc ccgagggcgt cgtcggccgg gtcgggatcg cggtctcgcc
     9061 cgcgcggccc gagcgcgcgt gggccgtcgt cgaagccgcg aagggcggcg tcttccgcac
     9121 ggacgacggc ggcgagacgt ggaagaaggt caacgacgag aacaagctcc gccagcgggc
     9181 ctggtactac tcgcacgtgg tcgccgaccc gaagaacgcg gacgcggtct acgtcctgaa
     9241 cgtcgggttc ttccgctcgg gcgacggcgg caagacgttt gacgcgatcc gcaccccgca
     9301 cggcgacaac cacgacctct ggatcgaccc ggacgacccg cagcggatga tcgagggcaa
     9361 cgacggcggc gcggccgtga cgttcaacgg cggccggagc tggtcgtcgg tcctgaacca
     9421 gcccacggcg cagttctacc gggtcgtcgc ggacgcccgt ttcccgtacc gcgtctacgg
     9481 cgcgcagcag gacaacacga cggtcgagat tctctcccgc acgcgcggcg gcggcatcgg
     9541 cccgtccgac tggcatccgg tcggcggctg cgagagcggc tggatcgccc cgaagccgga
     9601 cgacccggac gtgtccttcg cgggctgcta cggcggcgcg atcggccgct tcgacggccg
     9661 gacgggcgag gaacgcaacg tcgtcgcgtg gccgcagctc gcgatcgggc aggcgccgaa
     9721 ggacctgaag taccggttcc agtggaacgc gccgatcctg atctcgcggc acgactcccg
     9781 cacgctctac cacgcctccc aggtccttct caggagcacg gacgagggcg cgacgtggac
     9841 cgaggtcagc cccgacctca cgcggaacga gaaggcgacg caggggtact ccggcgggcc
     9901 gatcacgttc gacaacacgg gcgtcgaggt gtacggcacg atcttcgcgc tcgcggagtc
     9961 gccggccgag gccgggacga tctgggcggg gacggacgac gggctcgtgc agctcacgcg
    10021 cgacggcggg aagaactggg cgaacgtgac gccgaagggg cttccggagc gcatccaggt
    10081 caactccatc gaggtttcgc cgcacgacgc cggcgccgcg tggatcgccg cgacgatgta
    10141 caagcacgac gacgcgcggc cgtacctcta ccggacgagc gattacggga agacgtggac
    10201 gaagctcgtg aacggcatcc cggacggcgc gttcacgcgc gccgtgcgcg aggaccccgt
    10261 gcgcaagggg ctcgtcttcg cgggcaccga gcgcggcctc tacgtgtccc tcgacggcgg
    10321 cgccgcgtgg cagccgtttt cacggaatct gccggtggtc cccatcacgg atctcacgat
    10381 caaggacggc gacctcgttg tcgcgacgca gggccgcgcg ttctggatcc tggacgacct
    10441 ctcgccgctc cggcagtggg ccgacggcgt cgagaagaag gcggtccacg tcttcaagcc
    10501 gcgccccgcc gtccgcttcg ggtcggccgg ccgcggcg
//
LOCUS       DAOMFY010000125        10502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1942_length_10502_cov_7.199962, whole genome shotgun sequence.
ACCESSION   DAOMFY010000125 DAOMFY010000000
VERSION     DAOMFY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1942_length_10502_cov_7.199962"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2185)
                     /locus_tag="PLB01_11120"
     CDS             complement(<1..2185)
                     /locus_tag="PLB01_11120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ77898.1"
                     /translation="MTPERWREAQRIFHEARERPPAERRAFVTSAASGDAEMEAEVAS
                     LLAADEGAGEFLETPAAPSERPRTRVGPWQILREIGHGGMGTVYLGERADGPFRKTVA
                     VKVVRRGMDTEAVLRRFRQERQILAGLEHPNIARLLDGGNTEDGLPYLVMEHVEGEPL
                     TAWCARRALDVPQRLALFRTVCATVQFAHQNLVVHRDLKPGNIYVTADGTPKLLDFGV
                     AKLLNADLAGGTRDLTVAGGGGFTPEYASPEQIRGERITTASDVWSLGVLLYELLAGV
                     RPFGAAGAPPGEVAKAVLETEPERPSAAAARAGGERMRRRLEGDLDTIVLKALRKEPE
                     RRYASAEQLSEDVRRHLEGLPVKARGDTFAYRTGRLFARHRALAASAALVLLALVGGI
                     LATAHQARIARAERARADRRLEDVRKLAGSFLFEFHDAIATLPGSTPARELVVRRALE
                     YLGTLARETSGDPAAERDLADAYERLAEVQGGGSGPNLGDTKGAVASQRAALAIRETL
                     AARSGAGAREREALAVSHGMLSKLLLKSWELGPAHDEGRKAVALLEEVAKADSSPRIL
                     LRIGGSYHNLGLLEDLLGRRREALAALGKSTASYTAAAAARPGDTDARRGLALATFET
                     ASVLGHIGDAEAARTQGEKAVAIYESLLAGDPSNARIKMDLAMALHDVGEYAESLKDV
                     RGAAARHRRALTLVEELAAADPRDARAQVAIAYCANGYGQALARGG"
     gene            complement(2182..2751)
                     /locus_tag="PLB01_11125"
     CDS             complement(2182..2751)
                     /locus_tag="PLB01_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ77899.1"
                     /translation="MAGDPSSDVTRLLREWSAGDPSAADRLLPVVYGELHRLAAGYLK
                     RERTGHTLQPTALVNEAWLKLAGQGSPWQNRAHFLGVAAQAMRRILVDHARRRQAQKR
                     GGGELRVTLTDGVAARSGDEVDLVRLDAALERLSALDDRQARMVTMRFFAGLSVEETA
                     EALGVSEKTVKRDWAAAKAFLHRELGGER"
     gene            2842..3312
                     /locus_tag="PLB01_11130"
     CDS             2842..3312
                     /locus_tag="PLB01_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77900.1"
                     /translation="MRSAPLLALAVSLAGAAAFAAVASPPLEERDVVWQEAEQGGATL
                     PEPSVADVSAGCRATTGVARSGGALFSLRCPGSLGTKSAHPAYANLHLKNGWKVKDTR
                     VVVVGSSKAGFDLEKPAPGSDQPFVKGKLWAGPGGEVLIEVYVKVEGPRGTRPY"
     gene            3326..4141
                     /locus_tag="PLB01_11135"
     CDS             3326..4141
                     /locus_tag="PLB01_11135"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77901.1"
                     /translation="MKTIYAVLAGAFFAASAVAAPPELKPKLAVTKTGNGQGTVRSAA
                     GGIDCGAKCSNDYTLGVPVRLEAAPGTGLFAGWSGDCAGTNPVCSFAMDRSRTVTARF
                     VLPKLDVKKQREVKDARVVSNGNAIDCGARCSAELAAGSRVTLEAKDLPESWFAKWSN
                     SATGPRTEVDMSAMVVGISLQGDASFQAVLVGSRIAFRVEGPNGSLGRCDVESETHCA
                     LALQKGRTFKIVSESGPLNITVQPGGSRCMGKETCDVTLSLDRPTTVSVTRSR"
     gene            4188..5117
                     /locus_tag="PLB01_11140"
     CDS             4188..5117
                     /locus_tag="PLB01_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77902.1"
                     /translation="MKSTKPAVAFAALALAGIPASAQVKEYKPPAPQVAPALDKIAMT
                     EFASEPATIPPGATKVKLWVTVKNLTGGPQAVTINGPKIRILRTTPSPDALELETAVV
                     NLAPGATQRVGQDVNIAPGTREYFARVDPDDTLHEPLVQRANNEKRLRLAVPQISGQQ
                     AAAPGSQPQLETQALDYQRAKNAGAQFSNGIEGSQGGCVTVGQLNSVNWVVDHSPGVV
                     FIADCSLVPNGVRATPEAFTGFRLKNGWRVKDYSIGENIHGGNADWQWRQVPAKGTDD
                     PSMKMHVWASANGRAKVHVKVTIEGPAGTNPYQ"
     gene            5217..10442
                     /locus_tag="PLB01_11145"
     CDS             5217..10442
                     /locus_tag="PLB01_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77903.1"
                     /translation="MRRFLGNGSLRAGLLVAGVLLLPAGRAEATVILTPITGSVFPTQ
                     SVPVTALISSGNSLPAGFHPLVITGLPAGVTTVPAAPGYNGAAGIPITTAVVNFQFAV
                     GAGAVAGGPFTITVRDTVTASQATFNLFILEPKLNLSITTPTITLGSSTVNVLVRVSP
                     DPGFGANVGAAGVPLLFSVDNVPLPPSTPANVTAGGQKVELAPYNTTLGFPFSRTGAV
                     VPGSYTVPVIAFWTGTTRLTQSASAILTLNIPDIGVTGPGPFTVCNGSPAGRGLYTFN
                     SLFGYSGNALINPVSVPAGITETSPKSLTLTAGQSLGGQSFAMQAAGAALGGQSVTYR
                     IQDAAAAVDKTFPVAFRVVNPDVAASTPSASISLQAGGPSQAFTANTAPPDLSCRVSL
                     SVDYTVTGLPAGFTPPGTVSVSPAGFSYPAATLPISAGAGVTPGSYPANVHFNVPATG
                     QSGNVPVTVNVSAGPDFTLGAAPNPITIRQGQSGTVAISLTPLNGFAGAANVTIPAIQ
                     SITPNPSSFRLASGQPQNVVFTVAANAAPGTYTASVNGTAAGVPGTRTVNVSIVVPPP
                     PDFTLTASAPTITLTPGGTGKLTFTMTALNGWNTPTTFAVPTIPNVTATPAALSFPGT
                     GGAIDVTFAASPAAPNGTTPATFTASVPPTAGPPTTRSATVNVVIQPAPDFSLTVAPL
                     SLKVLQGDAGTVAVTANPLYGFTGTVAVTMPAVGGVSFVPQTFSLAAGASANVQVVTT
                     AATPAGTSTITLSGTAPGVAGTRTGAFSLTVDPKPDFNLAVTPPSVTLAPLGTAQVTV
                     TVLGLNNLTGVVDVAAPTTIQGVSFSPPSATIPVGGSATFTVTAGASATGSAGGSFIG
                     TSAAVSGPRTSTFVVAITAAPDFQVQLTPPSLVLPAGTAGTSSLSIVPINGFAGTFNV
                     ALAPPSGITVAPQSIPNVAASVPNAVVVTVAPGVSGVFDIPVSGTSTQNGLVRTATLR
                     VTVPAPDYVLTASPASLQLAPGGSAPVTITAAPINGFSGVVTVVAALPPGVDVDLKSF
                     TLAPGESKTVTVSLLPGAASFVSLPFNGTTPGGPSHAASVAISSQTTPDFTLTVTPPV
                     INLPAGGRASAQVTLVPLNGWSSAVDVTATGSSGISVVPPAFPLLPGVPYPVEIRAAD
                     DAPAGAVTLFFKASGANGGAGVTVTRTVNVQVTVAAADFNVRVTPPAPSVVAGRTADL
                     SLLLEPIGGFGGTVVVTPVSMPPGVTLTPPQPSLTPNLPQAATLSIPRGLAPGAYTLV
                     FRADEVPSPNVRRRPLLISKTLNVPLTVLAASGGFTVTVSPSTALVSPDQAVAVRYEL
                     RSLSDAPLTITGDTYLLRDRSGTVLGSVEEPLSLVLPPRGTVIVSNTVFVTGEQFGKA
                     GSPPVVLADRTFRATPDATGYVPNATATVTATAANSLLATASATRISIVYPPSGTLVG
                     RGNSLRAQGLVVGSGTGNLLVGWFFDGILVETATVPLQNGTPTSVSNAITLPTLISGN
                     HEIALSVLAPNTLSSPPVQIYVEEGQQTLRLVSPTAGAVLSPAFGAPTFAWIPVPGIA
                     RYGVGLRRRAPGAPWRWSYTTDTRWSPPASLWTSLSEGEYEWGVRGFTNTGRAFLDSQ
                     SGGASAPPTSEGSLDMAEGWTVSSAQGRFSIGGSEKALQDLHGRATADTAGLRFVWDE
                     LAGAMYVHTLYEQAPDGLKRVRTELLNKPQLLIPSAALPRGGPLLWRVTALDKEGRAL
                     GTTAAAPVPVAGGAR"
BASE COUNT         1459 a         3860 c         3662 g         1521 t
ORIGIN      
        1 cgccgccgcg ggcgagcgcc tggccgtagc cgttggcgca gtaggcgatc gcgacctggg
       61 cgcgcgcgtc ccgcgggtcg gctgccgcga gctcctcgac gagggtgagc gcgcggcggt
      121 ggcgcgcggc cgccccgcgg acgtccttca gggattccgc gtactccccg acgtcgtgga
      181 gcgccatcgc gaggtccatc ttgatgcgcg cgttcgaggg gtcgcccgcg aggagcgact
      241 cgtagatcgc gaccgccttc tctccctggg tccgcgcggc ttccgcgtcg ccgatgtggc
      301 cgaggacgga cgccgtctcg aaggtcgcga gggcgagccc gcgccgcgcg tcggtgtccc
      361 cgggccgcgc cgcggccgcc gcggtgtagg acgccgtgga cttcccgaga gccgcgagcg
      421 cctcgcggcg ccggccgagg agatcctcga ggaggccgag attgtggtag ctgccgccga
      481 tgcggaggag gatccgcggc gaggaatccg ccttcgcgac ctcttcgagg agcgcgacgg
      541 ctttccggcc ctcgtcgtgt gcgggcccga gctcccagga tttgaggagg agcttgctga
      601 gcatcccgtg cgagaccgcg agggcctcgc gctcgcgggc gccggcgccg gaacgcgccg
      661 cgagcgtttc gcggatcgcg agcgcggcgc gctggctcgc gacggcgccc ttcgtgtctc
      721 cgaggttggg cccgctcccg cccccctgca cctcggcgag gcgctcgtac gcgtcggcga
      781 ggtcccgttc ggcggccggg tcgcccgacg tttccctcgc gagcgtcccg aggtattcga
      841 gcgcccgccg cacgacgagc tcccgtgcgg gcgtcgagcc cggcagggtc gcgatcgcgt
      901 cgtggaactc gaacaggaac gagcccgcga gcttgcggac gtcctcgagc cgccggtccg
      961 cgcgcgcgcg ctccgcccgt gcgatgcgcg cctggtgcgc cgtcgcgagg attcccccga
     1021 cgagcgcgag gaggacgagc gcggcggacg cggcgagggc ccggtggcgc gcgaagagcc
     1081 tgcccgtgcg gtacgcgaac gtgtcgccgc gcgccttcac ggggaggccc tcgagatgcc
     1141 gccggacgtc ctccgagagc tgctcggcag acgcgtaacg acgctcgggc tccttgcgga
     1201 gggccttgag gacgatcgtg tcgaggtcgc cttccagccg ccgcctcatc ctctcgccgc
     1261 cggcgcgcgc ggccgcggcg ctgggcctct ccggctccgt ctcgaggacc gcctttgcaa
     1321 cctcgccggg cggggccccc gcggcgccga acgggcggac gcccgcgagg agctcgtaga
     1381 ggaggacgcc gagcgaccag acgtcggacg ccgtcgtgat gcgctcgccg cggatctgct
     1441 ccgggctcgc gtactccggc gtgaagcctc ctccgccggc cacggtcagg tcgcgcgtcc
     1501 cgcccgcgag gtcggcgttc aggagcttcg cgaccccgaa gtcgaggagc ttgggagtcc
     1561 cgtccgccgt cacgtaaatg tttccgggct tgaggtcccg gtggacgacg aggttctggt
     1621 gcgcgaactg gacggtcgcg cagaccgtcc ggaagagcgc gaggcgctgc ggcacgtcga
     1681 gcgcgcggcg cgcgcaccag gccgtgagcg gctcgccctc gacgtgctcc atgacgaggt
     1741 acggaaggcc gtcttccgtg ttcccgccgt cgaggaggcg cgcgatgttc gggtgctcga
     1801 ggccggcgag gatctgtcgc tcctggcgga agcgccggag caccgcttcc gtgtccatcc
     1861 cgcggcggac gaccttgacg gcgacggtct tgcggaaggg cccgtcggcg cgttcgccga
     1921 ggtacacggt ccccatgccg ccgtgcccga tctcgcgcag gatctgccag gggccgacgc
     1981 gcgttcgggg acgttcggaa ggagccgccg gggtctcgag gaactccccg gcgccctcgt
     2041 ccgccgcgag gagcgacgca acctcggcct ccatttccgc gtcaccgctc gcggcgctcg
     2101 tcacgaaagc gcggcgctcc gccggcggcc gctcccgcgc ttcgtggaag atgcgctgcg
     2161 cctcgcgcca gcgctcgggc gtcaccgctc gcccccgagc tcgcggtgga ggaacgcctt
     2221 cgccgcggcc cagtcgcgct tgaccgtctt ctcggagacg ccgagggcct cggccgtctc
     2281 ctcgaccgag aggcccgcga agaaccgcat cgtgaccatc cgggcctgcc ggtcgtccag
     2341 agccgagaga cgctcgagcg cggcgtcgag gcgcacgagg tccacctcgt cgccgctccg
     2401 ggcggcgacg ccgtccgtca gcgtcacgcg cagctcgccg ccgccgcgtt tctgggcctg
     2461 ccgccggcgc gcgtggtcca cgaggatccg ccgcatcgcc tgggccgcga cgccgaggaa
     2521 gtgcgcgcgg ttctgccacg gcgagccctg tcctgcgagc ttgagccagg cctcgttcac
     2581 gagagccgtc ggctggaggg tgtggccggt gcgctcgcgc ttcagatacc cggccgcgag
     2641 gcggtggagc tcgccgtaga cgacggggag cagccggtcc gcggcggacg ggtccccggc
     2701 gctccactcg cgaagcagtc gcgtcacgtc ggaggacggg tcgccggcca tcgtcttgtt
     2761 cctgcgccgg agattctagt cgggacgatg acccctccgg gccacggacc ccgcgttccc
     2821 gggagaagga gcccctcccg gatgcgcagc gccccactcc tcgctctcgc ggtctcgctg
     2881 gccggcgccg cggccttcgc cgcggtggcg agccctccgc tcgaggagcg ggacgtcgtc
     2941 tggcaggagg ccgagcaggg cggcgcgacg ctccccgagc cctcggtcgc ggacgtctcg
     3001 gccggctgcc gcgcgacgac cggcgtcgcg cggtccggcg gcgcgctctt ctcgctgcgc
     3061 tgcccgggct cgctcgggac caagtccgcc caccccgcgt acgcgaacct ccacctgaag
     3121 aacggctgga aggtgaagga cacgcgcgtc gtcgtcgtgg ggtcctcgaa ggcgggattc
     3181 gacctcgaga agcccgcgcc cggctccgac cagccgttcg tgaaagggaa gctctgggcg
     3241 gggccgggcg gcgaggtcct catcgaggtg tacgtgaagg tcgaagggcc gaggggcacc
     3301 cgaccctact gaggagggaa gcaccgtgaa aacgatctac gcagtactcg ccggcgcgtt
     3361 cttcgccgca agcgcggtcg ccgcgccgcc cgagctgaaa ccgaagctcg ccgtcacgaa
     3421 aaccggaaac ggccagggca ccgtacgttc ggccgccgga ggaatcgact gcggggccaa
     3481 gtgctcgaac gactacacgc tcggggtccc cgtccgactc gaggcggcgc ccggcacggg
     3541 gctcttcgcg ggttggtccg gcgactgtgc gggcacgaac cccgtctgtt cgttcgcgat
     3601 ggaccgaagc cgcaccgtaa cggcgcgttt cgtgctcccg aagctcgacg tgaagaagca
     3661 acgggaggtc aaggacgccc gtgtcgtctc gaacgggaac gcgatcgact gcggcgcgag
     3721 gtgctcggcc gaactcgccg ccgggagccg cgtcacgctc gaggcgaaag accttcccga
     3781 gagctggttc gcgaagtggt cgaacagcgc gaccggtccg cgtaccgagg tggacatgtc
     3841 cgcgatggtc gtggggatct cgctgcaggg cgacgcgtcg ttccaggccg tcctcgtcgg
     3901 atcgcggatc gcgttccggg tcgaaggccc gaacggctcg ctgggtcgct gcgacgtgga
     3961 gtcggaaacc cattgcgccc tcgcgctcca gaagggaaga accttcaaga tcgtgagcga
     4021 gtccggtccc ctgaacatca ccgtccagcc cggcgggtcc cgttgcatgg gaaaggaaac
     4081 atgcgacgtc acgctgtcgc tcgatcggcc gacgacggtc agcgtgacga ggtcgcgatg
     4141 aagaggatgc gagccggacg tccaaatgcc agaagaggag gctgtccatg aagtcgacaa
     4201 agcccgccgt cgctttcgcc gcactcgcgc tcgccgggat tccggcgtcc gcgcaggtca
     4261 aggagtacaa accgcccgct ccccaggtgg cccccgctct cgacaagatc gccatgacgg
     4321 agttcgccag cgagcccgcg acgattccgc ccggggcgac gaaggtaaag ctctgggtca
     4381 ccgtgaagaa cctcacgggc ggaccccagg ccgtcacgat caacggaccg aagatccgca
     4441 tccttcggac gacgccgtca cccgacgcgc tcgagctcga gacggcggtc gtgaacctcg
     4501 cgcctggcgc gacgcagagg gtcggacagg acgtgaacat cgcccccggc acgcgcgagt
     4561 acttcgcgcg ggtggacccc gacgacactc tccacgagcc gctcgtccag cgggcgaaca
     4621 acgagaagcg gctgcggctc gcggttccgc agatctcggg ccagcaggcg gcggcgccgg
     4681 gttcgcagcc gcagctcgag acccaggcgc tcgactatca gagggcaaag aacgcggggg
     4741 cgcagttctc gaacggcatt gaaggttcgc agggcggatg tgtcacggtg ggccaattga
     4801 acagcgtgaa ctgggtcgtg gaccacagcc ccggcgtcgt cttcattgcc gactgctcgc
     4861 tcgtgccgaa cggagtccgt gcgacgcccg aagccttcac gggcttccgc ctgaagaacg
     4921 ggtggagggt aaaggactac tcgatcggcg agaacattca cggcggaaac gccgactggc
     4981 aatggcggca ggtcccggcg aagggcacgg acgacccttc gatgaagatg cacgtatggg
     5041 cgagcgccaa cgggcgcgcc aaggtccacg tgaaggtcac gatcgaaggc cccgccggga
     5101 cgaaccccta ccagtgaccg aacgagtttc ggtgatgctg cgctgcagaa tgaggcgtgg
     5161 atgcggtccg cgcctcctcc gtggcaggat tgcgggattc tgagggggac atcacgatga
     5221 ggcgtttcct cggaaacggg agcctgaggg ccggactgct ggtggccggc gtccttcttc
     5281 ttcccgcggg ccgcgcggag gcgacggtca tcctcacgcc gataaccggg agcgttttcc
     5341 cgacgcagtc ggttccagtc acggcgttga tctcgagcgg gaactcgctg ccggccggat
     5401 tccatcccct cgtcatcacg ggcctcccgg ccggcgtgac gacggtcccg gcggctcccg
     5461 ggtacaacgg cgcggcaggg atcccgatca caacggcggt cgtgaacttc cagttcgccg
     5521 tcggcgcggg cgccgtcgcg ggcgggccgt tcacgattac cgttcgggac accgttaccg
     5581 cgagccaggc gacgttcaac ctattcattc tcgagcccaa gctcaatctc tcgatcacga
     5641 cgccgacgat cacgctcggg tcgtcgaccg tgaacgtcct cgtgcgcgtc tcgccggacc
     5701 ccggattcgg agcgaacgtg ggcgcggccg gcgtcccgct gctgttctcg gtcgacaacg
     5761 tgcccctccc gccgtcgacg cccgcgaacg tgacggccgg cggccagaag gtcgagcttg
     5821 caccctacaa cacgacgctc ggctttccct tcagccggac gggtgccgtc gtgccgggca
     5881 gctacacggt tcccgtcatc gcattctgga cgggcacgac gcgcctgacc cagagcgcaa
     5941 gcgcgatcct gacgctgaac atcccggaca tcggagtcac cgggccgggg cccttcaccg
     6001 tgtgcaacgg gagccccgcc ggcaggggcc tttacacgtt caacagcctg ttcggatact
     6061 ccgggaacgc attgatcaac ccggtctccg tgccggcggg gatcaccgag acgtcaccga
     6121 agtccctcac gctcacggcc gggcagagcc tgggaggcca gagtttcgcg atgcaggccg
     6181 cgggtgcggc gctgggcggt cagtccgtca cgtaccggat tcaggacgcg gccgcggccg
     6241 tcgacaagac gttcccggtc gcgttcaggg tcgtcaaccc cgacgtcgcc gcgtcgaccc
     6301 cgtcggcgtc catctcgctg caggcgggcg gcccgagcca ggctttcacc gcgaacacgg
     6361 ccccgccgga cttgagctgc cgcgtctcgc tttccgtgga ctacaccgtg accgggctgc
     6421 cggcaggctt cacgccgccg ggcaccgtga gcgtcagccc cgcgggattc tcctatcctg
     6481 cggccacgct gccgatctcg gcgggcgccg gcgtcacgcc gggctcgtac cccgcgaacg
     6541 tccacttcaa cgtcccggcc acgggccagt ccggaaacgt cccggtcacc gtgaacgttt
     6601 ccgcgggccc cgacttcacg ctcggcgcgg cgccgaaccc gatcacgatc cggcaggggc
     6661 aatcgggcac cgtcgcgatc tcgctgacgc ccctgaacgg cttcgccggt gcggcgaacg
     6721 tcacgattcc cgcgatccag agcatcacgc cgaacccgtc ttcgttccgg ctcgcgtccg
     6781 ggcagccgca gaacgtcgtc ttcacggtcg ccgcgaacgc cgcgccgggc acgtacacgg
     6841 cgtccgtgaa cgggaccgcg gccggcgtgc cgggcacccg aacggtcaac gtctcgatcg
     6901 tcgtgccgcc cccgccggac ttcacgctca ccgcgagcgc gcccacgatc acgctgacac
     6961 ccgggggaac gggaaagctc acgttcacga tgaccgcgct gaacggctgg aacacgccga
     7021 cgacgttcgc ggtccccacg atcccgaacg tcacggcgac acccgcggcc ctgagcttcc
     7081 cggggaccgg cggcgcgatc gacgtgacgt tcgccgcgtc gcccgcggcc ccgaacggga
     7141 cgaccccggc cacgttcacg gcgtcggtgc cgccgacggc cgggccgccg acgacgcgct
     7201 cggcgacggt caacgtcgtc atccagccgg cgccggactt ctcgctcacg gtcgcgccgc
     7261 tgtcgttgaa ggtcctgcag ggcgacgcgg ggaccgtggc cgtcacagcg aacccgctct
     7321 acggtttcac gggcacggtc gccgtgacga tgcccgccgt gggcggcgtc tcgttcgtgc
     7381 cccagacgtt ctcgctcgcg gcgggcgcga gtgcgaacgt gcaggtcgtc acgacggccg
     7441 cgacccccgc cggaacgagc acgatcacgc tttcggggac cgcccccggc gttgcgggaa
     7501 cgaggaccgg ggcgttctcg ctcaccgtcg acccgaagcc ggacttcaac ctcgccgtga
     7561 cgccgccgag cgtgacgctc gcaccgctcg gtacggctca ggtcaccgtg acggtcctgg
     7621 gcctcaacaa tctcaccggg gtcgtcgacg tggccgcgcc gacgacgatt cagggcgttt
     7681 ccttcagtcc gccctccgcc acgattccgg tcggggggag cgcgacgttc accgtcacgg
     7741 cgggcgcatc ggcgacggga tcggcaggcg gcagcttcat cggcacgtcg gcggccgtct
     7801 ccggcccgcg aacttcgacg ttcgtcgtcg ccatcacggc ggcgcccgac ttccaggttc
     7861 agctgacccc tccctccctc gtccttccgg ccgggacggc gggaacttcg agcctctcga
     7921 tcgtcccgat caacgggttc gccggaacgt tcaacgtcgc gctcgcacct ccttccggaa
     7981 tcacggtcgc accccagtcg attccgaacg tggcggcaag cgtgccgaac gcggtggtcg
     8041 ttacggtcgc accgggagtg tcgggcgtct tcgacatccc ggtgtcgggc acctcgacgc
     8101 agaacggact cgtgcgcacc gcgacgctgc gcgtcacggt cccggccccg gactacgtcc
     8161 tgacggcgtc cccggcgtcg ctccagctcg cgcccggggg ctcggcgccc gtgacgatca
     8221 cggcagctcc gatcaacggc ttctccggcg tggtcacggt cgtcgcggcg cttcctccgg
     8281 gcgtcgacgt ggacctgaag tcgttcacgc tcgcccccgg ggaatcgaag accgtgacgg
     8341 tctcgctgct gcccggcgcc gcctctttcg tgagccttcc gttcaacggc acgacgcccg
     8401 gcggcccgtc gcacgcggcg agcgtcgcca tctcctccca gacgaccccg gacttcacgc
     8461 tcaccgtcac gccgcccgtc atcaacctcc cggccggggg acgcgcttcc gcgcaggtca
     8521 ccctcgtccc gctgaacggc tggtccagcg ccgtcgacgt cacggcgacc ggctcttcgg
     8581 gcatctcggt cgtcccgccg gcgttcccgc tcctgccggg cgtcccgtac ccggtcgaga
     8641 tccgcgcggc ggacgacgcg ccggccggcg ccgtcacgct cttcttcaag gcgtccggcg
     8701 cgaacggggg cgcgggtgtc accgtgacgc ggacggtcaa cgtccaggtc acggtcgcgg
     8761 cggcggactt caacgtgcgc gtgacgccgc ccgccccttc ggtggtggcg ggccggaccg
     8821 cggacctctc gctcctcctc gagccgatcg gcgggttcgg cggcaccgtg gtcgtcacgc
     8881 ccgtctcgat gccgcccggc gtgacgctca cgccgccgca gccctcgctc acgccaaacc
     8941 tcccgcaggc cgcgacgctt tcgatccccc gcggcctggc ccccggcgcg tacacgctcg
     9001 tgttccgcgc ggacgaggtg ccgtcgccga acgtgcggcg acgtccgctc ctcatctcga
     9061 agacgctcaa cgtcccgctc acggtcctgg cggcctccgg cggattcacg gtcaccgttt
     9121 ccccgtcgac ggccctcgtg tcgccggacc aggccgtcgc cgtccgctac gagctccgca
     9181 gcctctcgga cgcgccgctc acgatcacgg gcgacacgta cctgctgcgc gaccggtccg
     9241 gaacggtgct cggctccgtc gaggagccgc tctcgctcgt gctcccgccg cgaggcaccg
     9301 tgatcgtctc gaacacggtc ttcgtgaccg gcgagcagtt cggaaaggcc ggctccccgc
     9361 cggtcgtcct cgcggaccgc acgttccgtg cgactcccga cgcgacgggc tacgtcccga
     9421 acgcgacggc gacggtcacg gccacggccg cgaactcgct gctcgcgacg gcctcggcca
     9481 cgcgcatttc gatcgtctat ccgccgtccg gaacgctcgt cgggcgcggc aactccctgc
     9541 gcgcccaggg gctcgtcgtg gggagcggca cgggcaacct gctcgtcggc tggttcttcg
     9601 acggcatcct ggtggagacg gcgacggtgc cgctgcagaa cggcacgccg acgtccgtgt
     9661 cgaacgcgat cacccttccc acgctgatct cgggaaacca cgagatcgcg ctctcggtcc
     9721 tcgcgccgaa cacgctctcg tcgccgccgg tccagatcta cgtcgaggaa ggccagcaga
     9781 ccctgcggct cgtctcgccg accgcgggcg cggtcctctc tccggcgttc ggtgcgccga
     9841 cgttcgcgtg gattccggtg ccgggcatcg cccgctacgg cgtcggcctg aggcggcgcg
     9901 ctccgggagc gccgtggcgc tggtcctaca cgaccgatac gcgctggagc ccgccggctt
     9961 ctctctggac gtcgctctcc gagggcgagt acgaatgggg cgtgcgcggc ttcacgaaca
    10021 cgggccgcgc gttcctcgac tcgcagagcg ggggcgcgtc cgcgccgccc acgagcgagg
    10081 gctcactcga catggccgag gggtggacgg tctcgtcggc gcagggacgg ttctcgatcg
    10141 gcggctcgga gaaggccctg caggacctcc acggccgcgc gaccgccgac acggcgggct
    10201 tgcggttcgt ctgggacgag ctcgccggcg cgatgtacgt ccacacgctc tacgagcagg
    10261 cgccggacgg cctgaagcga gtccggaccg agctcctaaa caagccgcag ctcctgattc
    10321 cgtccgccgc tctgccgcga ggcgggccgc tcctgtggag ggtcaccgcg ctcgacaagg
    10381 aggggcgtgc gctcggcacc accgccgccg cgcccgttcc ggtcgcggga ggcgcgcggt
    10441 gaggcgcgcc ccggccctct ccacgctcgc cgcggcgctc acggccctcg cgggagccgc
    10501 at
//
LOCUS       DAOMFY010000126        10481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1947_length_10481_cov_6.811241, whole genome shotgun sequence.
ACCESSION   DAOMFY010000126 DAOMFY010000000
VERSION     DAOMFY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1947_length_10481_cov_6.811241"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1819
                     /gene="thrS"
                     /locus_tag="PLB01_11150"
     CDS             11..1819
                     /gene="thrS"
                     /locus_tag="PLB01_11150"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQQ77904.1"
                     /translation="MTPKHPDALELYRHSTAHLCANAVKRLFPGVKIGIGPAIDNGFY
                     YDFDPGRPFTPEDLEKIEAEMRRIVAEDNVVVRKEMSKAEAVKLFQGQNDPLKVEIVD
                     GIPEGSLSCYEQKDFIDLCRGPHVPSTGKLGVFKLTHSAGAYWKGDEKNPMLQRIYGA
                     VFLTQKDLDEHLQRLEAARARDHRKLGKELGLFVFHHWAPASPFFLPKGATVYNLLVA
                     FMRDLYVKYGYQEVVTPQIFDVDLFKTSGHYQNYHDNMYFTTIDEREFGVKPMNCPGH
                     FLMFASQAWSYRDLPVRFADFGRLHRYEKSGVTQGLTRVRTFAQDDSHIFAAIDQMAD
                     EMNRFLDFIREVYETFAFKEVKVGLGTRPEKFMGEIENWDKAENALKDAFEKRAASLA
                     GWSYFLNAGDGAFYGPKLDFQVTDAIGRAWQLGTLQVDFSNPERFDLHYTAEDGSKKR
                     PVVLHRAILGSLERFFGILIEHTGGDFPFWLAPEQVRVVPVSEKFNEYGEKEILRRLR
                     EAGIRASIDARNEKLGAKIRLGEMEKIPALLIVGEKEVAAGTVSLRVRHGGEFKDQKI
                     EELLRMMRGAVATRSLQWHAPAEAPATPSSPSKKIS"
     gene            1917..2417
                     /gene="infC"
                     /locus_tag="PLB01_11155"
     CDS             1917..2417
                     /gene="infC"
                     /locus_tag="PLB01_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQQ77905.1"
                     /translation="MNEYILRFKGVRLISSTGEQLGVLEPQAALAKGREEGLDVVVIA
                     EQADPPVCKIMDYGKFTFEKKKKEHESKKKQKVTQVKEIKFRPNIDEHDYDFKFRNII
                     RFLEDGDKVKATVQFRGREMSRLDNGRKVLDRLIKDLEGKAHAESGTEMMGNRMHQIL
                     SPIKKH"
     gene            2440..2640
                     /gene="rpmI"
                     /locus_tag="PLB01_11160"
     CDS             2440..2640
                     /gene="rpmI"
                     /locus_tag="PLB01_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQQ77906.1"
                     /translation="MPGKRKTHRGAAKRFHKTATGKFKRGHAMKRHILTKKNASRLRG
                     LKKSTLVSKADTKNVRSMLPYA"
     gene            2667..3044
                     /gene="rplT"
                     /locus_tag="PLB01_11165"
     CDS             2667..3044
                     /gene="rplT"
                     /locus_tag="PLB01_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002507379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQQ77907.1"
                     /translation="MPRVKRGPKRKNRRVRVLKLAKGYYGTKHNCFRIAELQVERSLA
                     YATRDRKQKKREFRGLWIVRINAAARDHDLSYSKLMAGLKKAGSDVNRKVLAEIAVED
                     PKGFASLVASAKSALGLPAAANA"
     gene            3112..4155
                     /gene="pheS"
                     /locus_tag="PLB01_11170"
     CDS             3112..4155
                     /gene="pheS"
                     /locus_tag="PLB01_11170"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009351646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQQ77908.1"
                     /translation="MRTAQEKAATSGLLPAEVRALHVKWLGQQQGAITALLKQLRDVP
                     KDQKAAAGAAVNSLKKEAEAALSSLEESSVLFERVQKQKALAVDVTLPPRVPRLGRLH
                     PLTVVRRRMEEAFFALGYEVAHGPEVESDWHNFESLNFPPDHPARDAVDTFFVKDGGE
                     GRDRLLLRTHTSPVQIRTMRSQKPPIRIVIPGRVYRKDDIDPRHSPVFHQMEGLTVGE
                     GITFADLKGTLAAFSRRLFSPETKIRFGPTFFPFVEPGVDVAVSCVFCRGTGAANGAS
                     CRPCSGSGWIEIMGAGMVHPNVLRNGGIDPEKFSGFAFGMGIDRIAFLLHGVPDLRLF
                     FENDVRFLDRVWA"
     gene            4157..6325
                     /gene="pheT"
                     /locus_tag="PLB01_11175"
     CDS             4157..6325
                     /gene="pheT"
                     /locus_tag="PLB01_11175"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQQ77909.1"
                     /translation="MKFAYSWLASYLPGPAPDPGALGDRLTAVGFIVEGVEGSGASTV
                     YDVEITANRPDGMNHRGLAREAAVAMRRAFADAEASVPVPETGPAVETRAAVVVAEPS
                     LCSRYSARVIEGVTIRPASASVTARLAAIGQSAISSPVDATNHVLWDIGQPLHAFDLD
                     ALAKGADGLPTIVVRRGKAGESLVTLDGVERKLGPQHLVIADAERPVALAGVMGGLAT
                     AISEKTTRILLESAHFHPGAVRKTARSLGMHTDASHRFERGTDPEATLDGLARAARLI
                     ALDCGGSVAKGVIDVRAERAAPATLSLRLARLASFLGMTIPEGRVVGILAELGLAPAL
                     AGGAVNVTVPSWRIDLTSEEDVVEEVIRCEGYDRLPETLPAPFVPRTLSRAAQVPERA
                     RDLLAGMGLLECATYSFVSEAENAPFASSAPGPPVVVENPLGEPFTTLRATPVIGLLQ
                     SAQHNVRRGMKDLALFEVGTSFGWNPEKKEKEKISSEGREKREDAIGDGRVVERTRVG
                     ILLAGARGRHWSQTEAANSDFFEGAGIAEALMRGLGLGEPRAPITFKEDLRFSSFLAS
                     GRAASIEVGGVPCGWVGVLEPALAASWDLVDPVVGDIDLGVLAAALPPAETSVEPPSK
                     FPGSEVDLTATHALALPWAAIEAAVRTGAPAELLAVEAKGRYRGPGVPDGFVKTTLTL
                     RFGSPRGSLAREDVNGWRDAAARRLLALPGVKVDGVPAPA"
     gene            6335..6619
                     /locus_tag="PLB01_11180"
     CDS             6335..6619
                     /locus_tag="PLB01_11180"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HQQ77910.1"
                     /translation="MKEAKGKNEKSEGSLEPVLDAIEERIEALVDVVKKLAAENARLE
                     SELAAAKAKADEGGEAAMLLARYEDERNAVKERIERVLRTLDEGAGAGKA"
     gene            6999..7178
                     /gene="ssrS"
                     /locus_tag="PLB01_11185"
     ncRNA           6999..7178
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLB01_11185"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7288..8877
                     /gene="rny"
                     /locus_tag="PLB01_11190"
     CDS             7288..8877
                     /gene="rny"
                     /locus_tag="PLB01_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQQ77911.1"
                     /translation="MTIAVEAALAVVAVGFVVWGLRIRGTASLARAEAELEAKKVREE
                     AKLAAEAAVRKAEAAAQKTELDAKERLLKAREAAEAEAAKRRAELLDQEKRLLQKEET
                     LDKKILTAEKREQDSENRAQNLTKREKRMEELEEEAKKAAEAVVAEQRAKLESIAGLT
                     AEQAKRDLIRELENEARLEATGLVRRIEEDAVESANWKAKRLLSMTIQRMASDHVVET
                     TVSVLDLPSDDMKGRIIGREGRNIRAVEAATGVDIIIDDTPEAILLSSFDPIRREVAR
                     RALERLIQDGRIHPARIEEVVEKVREELWQDVKTAGEAAAFEFGIADLHPEVVKLLGR
                     LKYRTSYGQSVLQHSKEVGWAACHLASELGADVAVTKRAGLLHDIGKAVDREQEGTHL
                     ELGRQILKKYGESEAVIHGMECHHGDYEPRTIEAVLVNAADAISAARPGARREILETY
                     VKRLEKLEKMTEGMKGVERTFAIQAGRELRVIVDAKKISDDECLWMSKDIAKRVEAEL
                     QYPGQIKITVIRETRAVDFAK"
     gene            8874..9665
                     /locus_tag="PLB01_11195"
     CDS             8874..9665
                     /locus_tag="PLB01_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HQQ77912.1"
                     /translation="MKLLFVGDVVGKPGRRALGMLEKLIAREHVDFTVVNIENAAGGF
                     GVTAELFADMSKLPIDVFTSGNHIWDKKDFVPLLDDLENLLRPANYPPGNPGRGWTIQ
                     TTPAGVPVGVVNLQGQVFMANTDSPFRVGDAVLAEMRAKRPEMKVVLIDIHAEATSEK
                     QAIGWWFDGRASLVIGTHTHVPTADARILPKGTAYQGDAGMTGAYEGIIGFDPKNILE
                     KFLHQTPRSMETATRDVRLCGAIVDVDEATGKARTITSIQEKLAT"
     gene            9683..>10481
                     /locus_tag="PLB01_11200"
     CDS             9683..>10481
                     /locus_tag="PLB01_11200"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HQQ77913.1"
                     /translation="MRTRLLVLAVFAVTFAFAAEPLLTGRGTDFRAYLEAANALRAGH
                     DPYAGVYPYLYPPLLAVLLVPLTFLPPGAAAWLWAAASAAALAASAALVARGSARILV
                     LALLFAPFAATQWNLQANAFVLLLLVLARDRLDRGFEGRGGALLGLSIALKPFGVLAA
                     CALALTGRWRAALAAGAAAVLPFILVVPFLGGAGAAGAAGSVKRILASSWVETYGGNV
                     SLNGSLDRALPEGAGSSRHRAVGLAVAGWTAALVAAAAAGAARSGKRL"
BASE COUNT         1807 a         3579 c         3535 g         1560 t
ORIGIN      
        1 gttcgaggtc gtgacgccga agcaccccga cgccctcgag ctctaccgcc actccacggc
       61 gcacctctgc gcgaacgcgg tcaagcgcct tttcccgggc gtgaagatcg gaatcggccc
      121 cgcgatcgac aacggcttct actacgactt cgaccccggc cgtccgttta cgcccgagga
      181 cctcgagaag atcgaggccg agatgcggcg catcgtggcg gaggacaacg tcgtcgtccg
      241 caaggagatg tcgaaggccg aggccgtgaa gctcttccag ggccagaacg acccgctcaa
      301 ggtcgagatc gtcgacggca tccccgaggg cagcctctcc tgctacgagc agaaggactt
      361 catcgacctc tgccgcggcc cgcacgtgcc gtccacgggg aagctcggcg tcttcaagct
      421 cacgcactcc gccggcgcct actggaaggg cgacgagaag aacccgatgc tccagcgcat
      481 ctacggggcc gtgttcctca cgcagaagga tctcgacgag cacctccaac gcctcgaagc
      541 cgcccgggcc cgcgaccacc gcaagctcgg caaggagctc ggactcttcg tgttccatca
      601 ctgggcgccc gcgtcgccgt ttttccttcc gaagggtgcg acggtctaca acctcctcgt
      661 cgccttcatg cgcgacctgt acgtcaagta cggttaccag gaggtcgtga cgccgcagat
      721 cttcgacgtc gatctcttca agacgtccgg gcactaccag aactaccacg acaacatgta
      781 cttcacgacg atcgacgagc gcgagttcgg cgtgaagccc atgaactgtc ccgggcactt
      841 cctcatgttc gcctcgcagg cgtggtccta ccgcgacctg cccgtgcgct tcgcggactt
      901 cgggcgcctg caccgctacg agaagtccgg cgtcacgcag ggcctcacgc gcgtccgcac
      961 gttcgcgcag gacgactcgc acatcttcgc cgcgatcgac cagatggccg acgagatgaa
     1021 ccgcttcctc gacttcatcc gcgaggtcta cgagacgttc gcgttcaaag aggtgaaggt
     1081 cggcctcggg acgcgccccg agaagttcat gggcgagatc gagaactggg acaaggccga
     1141 gaacgccctc aaggacgcgt tcgagaagcg cgccgcctcg ctggccggct ggtcttattt
     1201 cctgaacgcg ggcgacggcg ccttctacgg gccgaagctc gacttccagg tcacggacgc
     1261 gatcggccgc gcctggcagc tcggtacgct gcaggtcgac ttctcgaacc cggagcgctt
     1321 cgacctgcac tacacggccg aggacgggtc caagaagcgc ccggtcgtcc tccaccgcgc
     1381 gatcctcggc tcgctcgagc ggttcttcgg gatcctcatc gagcacacgg gcggagactt
     1441 cccgttctgg ctcgcgccgg agcaggtgcg tgtggttccg gtgtcggaga agttcaatga
     1501 atacggagaa aaagagattc ttagaaggtt gagagaggcc gggattcggg cgagcatcga
     1561 cgcccgcaac gagaagctcg gcgccaagat tcgtctgggg gagatggaga agattccggc
     1621 tctcctcatc gtcggcgaga aggaagtcgc ggcggggacg gtgtccctcc gcgtccgcca
     1681 cggcggcgag ttcaaggacc agaagatcga agaattgctg cgaatgatga ggggcgcggt
     1741 ggccacgagg agccttcagt ggcacgcccc ggccgaggct ccggcgaccc cctcttcacc
     1801 ttctaagaag atctcttaag cgccatttca ttcaacgcgt cgccaacagt aaggaggaaa
     1861 tccccatcgc attcttcaga ggtggtcccc gggtcccccc gaccaagaat tccccgatca
     1921 acgagtacat cctgcgtttc aaaggcgtcc ggctgatctc gtccacgggc gagcagctcg
     1981 gcgtcctcga gccccaggcc gcgctggcga agggtcgcga ggaaggcctc gacgtcgtcg
     2041 tgatcgccga gcaggccgac cctccggtct gcaagatcat ggactacggg aagttcacgt
     2101 tcgagaagaa gaagaaagag cacgagtcca agaagaagca gaaggtcacc caggtcaagg
     2161 aaatcaagtt ccggccgaac atcgacgagc acgactacga cttcaagttc cggaacatca
     2221 tccggttcct cgaagacggg gacaaggtga aggcgacggt ccagttccgc ggacgcgaga
     2281 tgtcgcgcct cgacaacggg cggaaagtcc tcgaccggct catcaaggac ctcgagggca
     2341 aggcgcacgc cgagtccggc accgagatga tggggaaccg catgcaccag atcctgagcc
     2401 ccatcaagaa gcactagaga tcgaaagaag gagaccgtca tgcccggcaa gagaaagacg
     2461 caccgggggg ctgcgaagcg cttccacaag accgcgacgg ggaagttcaa gcgcggccac
     2521 gcgatgaagc gtcacatcct cacgaagaag aacgcgagcc ggctgcgggg gctgaagaag
     2581 tcgacgctcg tgtcgaaagc ggacacgaag aacgtccgct cgatgctccc gtacgcctga
     2641 tccgaaacca ctaaggagat acgaccatgc ctcgcgtcaa gcgtggaccc aagaggaaga
     2701 atcgccgcgt tcgcgtgctc aagctcgcga agggctacta cggaaccaag cacaactgct
     2761 tccgcatcgc cgagctccag gtcgagcgtt cgctcgccta cgcgacgcga gaccgcaagc
     2821 agaagaagcg cgaattccgc ggactgtgga tcgtgcgcat caacgccgcc gcccgtgacc
     2881 acgacctgag ctacagcaag ctcatggcag ggctcaagaa ggccggctcg gacgtgaacc
     2941 ggaaggttct cgccgagatc gccgtcgagg acccgaaggg cttcgcgtcg ctcgtcgcgt
     3001 ccgccaagtc cgcgctcggg cttcccgcag ccgcgaacgc ctgatccgcg tcgacgagtg
     3061 gcggatctgc tcgcttcggt cgaggagaat cgaagagtct tcttcgaaga gttgaggacc
     3121 gcgcaggaga aggccgcgac gagcggcctc ctcccggccg aggtgcgcgc gctgcacgtc
     3181 aagtggctgg gccagcagca gggagcgatc acggccctcc tcaagcagct ccgggacgtc
     3241 ccgaaggacc agaaggctgc tgccggcgcc gccgtcaact cgctgaagaa ggaggctgag
     3301 gcggccctct cttcactcga agagtcttcg gtgctcttcg aacgagtcca gaagcagaag
     3361 gccctcgccg tcgacgtcac gctccccccg cgcgtcccgc gcctcgggcg gctgcatccg
     3421 ctgacggtgg tccggcgccg catggaggag gccttcttcg cgctcggcta cgaggtcgcg
     3481 cacggccccg aggtcgagtc ggactggcac aacttcgagt cgctgaactt cccgcccgac
     3541 cacccggcgc gcgacgccgt ggacacgttc ttcgtgaagg acggcgggga aggacgcgac
     3601 cgccttctgc tgcgcacgca cacctcgccg gtccagatcc ggacgatgcg gtctcagaag
     3661 ccgccgatcc ggatcgtcat cccgggccgc gtctaccgca aggacgacat cgacccgcga
     3721 cactcgccgg tcttccacca gatggaagga ctgacggtgg gagagggcat cacgttcgcg
     3781 gacctcaagg gcacgctcgc cgcgttctcg cggcggcttt tctcgcccga gacgaagatc
     3841 cgcttcggtc cgacgttctt cccgttcgtc gagccgggcg tcgacgtcgc cgtctcgtgc
     3901 gtcttctgca ggggaaccgg cgcggcgaac ggcgcgtcgt gccgcccgtg ctccggctcc
     3961 gggtggatcg agatcatggg ggccgggatg gtccacccga acgtgctccg gaacggcggc
     4021 atcgaccccg agaagttctc cggcttcgcg ttcggaatgg ggatcgaccg catcgcgttc
     4081 ctcctccacg gggtcccgga cctgcgcctc ttcttcgaga acgacgtgcg gttcctggac
     4141 cgcgtgtggg cctgagatga agttcgcgta ttcctggctc gcctcttacc tgccgggccc
     4201 cgcgccggac ccgggggcgc tcggcgaccg cctgacggcc gtcggcttca tcgtcgaggg
     4261 cgtcgagggc tccggcgcat ccacggtcta cgacgtcgag atcacggcga accgccccga
     4321 cggcatgaac caccgcggcc tcgcgcgcga ggcggccgtg gcgatgcgcc gggcgttcgc
     4381 ggacgccgag gcgtccgttc cggtccccga aacggggccg gccgtggaga cgcgcgccgc
     4441 cgtcgtcgtg gccgaaccct ccctgtgctc gcgctactcc gcgcgtgtga tcgagggcgt
     4501 gaccattcgc ccggcttccg cctcggtcac ggcgcgcctc gccgcgatcg gccagagcgc
     4561 gatctcctcg cccgtcgacg cgacgaacca cgtgctctgg gacatcggcc agccgctcca
     4621 cgcgttcgac ctcgacgccc tcgcgaaggg ggccgacggc ctcccgacga tcgtcgtgcg
     4681 gcgcgggaag gcgggagagt cgctcgtcac gctcgacggc gtcgagcgca agctcggccc
     4741 gcagcatctc gtgatcgcgg acgccgagag gcccgtcgcc ctcgcgggcg tcatgggcgg
     4801 tctcgcgacg gcgatctcgg agaagacgac gcggatcctc ctcgagagcg cgcacttcca
     4861 tccgggggcg gtgcgcaaga ccgcgcgctc tctcgggatg cacacggacg cctcccaccg
     4921 cttcgagcgc gggacggacc ccgaggcgac gctcgacggc ctcgcccgcg ccgcgcgcct
     4981 catcgccctc gactgcggtg ggagcgtcgc gaagggcgtg atcgacgtgc gcgcggagag
     5041 ggccgccccg gcgacgcttt ccctccgcct cgcgcggctc gcgagcttcc tcggcatgac
     5101 gatccccgaa ggccgcgtcg tcgggatcct ggccgagctg ggcctcgcgc cggccctcgc
     5161 gggcggcgcc gtgaacgtga ccgttccgag ctggcgcatc gacctcacga gcgaagagga
     5221 cgtcgtcgaa gaggtcatcc ggtgcgaggg ctacgaccgc ctgcccgaga ccctcccggc
     5281 gccgttcgtg ccgcgtacgc tctcgcgcgc cgcgcaggtg cccgaacgcg cccgcgacct
     5341 cctcgcgggg atgggcctcc tcgagtgcgc gacgtactcg ttcgtttcgg aggccgaaaa
     5401 cgcgccgttc gcctcctcgg ctcccggccc gccggtcgtc gtcgagaacc cgctcggcga
     5461 gccgttcacg actctccgcg ccactcccgt catcggcctc ctccagagcg cgcagcacaa
     5521 cgtgcggcgc ggcatgaagg acctcgccct cttcgaggtg gggacgagtt tcggatggaa
     5581 tccggagaag aaagagaaag agaagatttc ttctgaaggt agagagaaga gagaagacgc
     5641 gatcggggac ggaagggtcg tcgagcgaac gagagtcggg atcctcctcg caggagcgag
     5701 aggccgccac tggtcgcaga cggaagcggc gaattcggat ttcttcgaag gggcggggat
     5761 cgcagaagcc ctcatgaggg gactaggctt gggtgagccc cgtgccccga ttaccttcaa
     5821 agaagatctt cggttctcct cgttcctcgc ctcggggcgt gccgcctcga tcgaggtcgg
     5881 gggtgttccc tgcggctggg tcggcgttct cgagcccgct ctcgccgcct catgggatct
     5941 cgtcgacccg gtcgtcggcg atatcgacct cggggtcctt gccgccgcgc tgcctcccgc
     6001 cgagacgtct gtggaacctc cctcgaagtt ccccggcagc gaggtggacc tcacggcgac
     6061 gcacgcgctt gctctgccct gggcggcgat cgaggcggcc gtccggaccg gcgcgccggc
     6121 cgagctactc gccgtcgagg cgaaggggcg ttaccgcggc cccggcgtcc cggacggttt
     6181 cgtgaagacg accctgaccc tgcgtttcgg atccccgcgc ggctcgctcg cgcgcgagga
     6241 cgtgaacggc tggcgcgacg cggccgcacg gcgccttctc gcccttccgg gcgtgaaggt
     6301 cgacggcgtg ccggcgcccg cctgaggagg acgcgtgaag gaagccaagg gaaagaacga
     6361 aaagtccgaa ggctccctcg agcccgttct cgacgcgatc gaggagcgga tcgaggcgct
     6421 cgtcgacgtc gtgaagaagc tcgccgccga gaacgcccgg ctcgaatccg agctggcggc
     6481 tgcgaaagcc aaggcggacg agggaggcga ggccgcgatg ctcctcgcgc gctacgagga
     6541 cgagcgcaac gccgtgaaag agcggatcga gcgcgttctc aggaccttgg acgagggagc
     6601 gggcgcgggt aaggcgtaag aatgcatgct atcctttccg cgcagctcga tgctgcagcg
     6661 gaatcatggc gaaacccagc gtcacacagg tcgagatctt cgggcagacg tactccgtcc
     6721 gcgccgcggg ggatcccggc tacatcaagg agctggccgg gttcgtcgac cggaagatgc
     6781 gcgaggtctc ggagcacgcg cccacgatcg acgccgcgaa gatcgccatc ctaaccgccc
     6841 tcaacatttc cgacgagttc taccagttcc gttccaagtc aggctccgag ggtgatccgg
     6901 gccgtctcgc cgcacgggcg gggcgtctcg tggaaaagct cgacgaggtc ctgaagacgt
     6961 ctccctcagt tccggcgagg gccggcaatg aatcgatcgg caccctgcgc ggtgcgtgaa
     7021 tgggatatgt ccttgaaccg atatatttat cgtggagcct tcgacatcgg gccgaccggt
     7081 gacgcccctt cgggggaact aaaggtctgc cccgggggct ccccctgtct tttgacgggt
     7141 tcaatcgacc tttcccacac ggccgaggcg gggggccctt cttgggccgc accggaggga
     7201 cgtctccagg atcccgtcgc gctctagttc cggatcaccc tctcaaacga acgtttcgag
     7261 ggggacagac ccgtgagtcc taatctgttg accatcgccg tcgaggccgc gctggcggtc
     7321 gttgccgtgg gcttcgtcgt ctggggcctg cggatccgcg ggacggcgtc gctcgcgagg
     7381 gccgaggccg agctcgaggc caagaaggtc cgtgaggaag cgaagctcgc ggccgaggcg
     7441 gccgtccgca aggccgaggc cgccgcccag aagacggagc tcgatgccaa ggagcggctg
     7501 ctcaaggccc gcgaggccgc ggaggccgag gccgccaagc ggcgcgccga gctcctcgac
     7561 caggagaagc ggctcctcca gaaggaagag acgctggaca agaagatcct gacggccgag
     7621 aagcgcgagc aggactccga gaaccgcgcc cagaacctca cgaagcgcga gaagcgcatg
     7681 gaggagctgg aggaagaggc gaagaaggcc gcggaggccg tcgtcgccga gcagcgcgcc
     7741 aagctcgagt cgatcgccgg cctcacggcg gagcaggcca agcgcgacct gatccgcgag
     7801 ctcgagaacg aggcccgtct cgaggcgacc ggcctcgtgc gccgcatcga ggaagacgcc
     7861 gtcgagagcg cgaactggaa ggcgaagcgc ctcctgtcca tgacgatcca gcgcatggcg
     7921 tccgaccacg tcgtcgagac gaccgtgtcg gtcctcgacc tgccgtccga cgacatgaag
     7981 ggccgcatca tcggccgcga gggccggaac atccgcgccg tcgaggccgc gaccggcgtc
     8041 gacatcatca tcgacgacac gcccgaggcg atcctcctct cgtcgttcga cccgatccgc
     8101 cgcgaggtcg cgcgccgcgc cctggagcgc ctcatccagg acggtcgcat ccacccggcc
     8161 cgcatcgagg aggtcgtcga gaaggttcgc gaggagctct ggcaggacgt caagacggcc
     8221 ggcgaggccg cggcgttcga gttcggcatc gcggacctgc accccgaggt cgtgaagctc
     8281 ctcggccgcc tcaagtaccg cacgagctac ggccagagcg tcctgcagca cagcaaggaa
     8341 gtcggctggg ccgcctgcca cctcgcgagc gagctcggcg cggacgtcgc ggtcacgaag
     8401 agggcggggc tcctccacga catcggcaag gcggtcgacc gcgagcagga aggcacgcac
     8461 ctcgagctcg gccggcagat cctcaagaag tacggcgagt ccgaggcggt catccacggc
     8521 atggagtgcc accacggcga ctacgagccg cgcacgatcg aggcggtcct cgtgaacgcc
     8581 gccgacgcga tttccgccgc gcgcccgggc gcgcgccgcg agatcctgga gacctacgtc
     8641 aagcgcctcg agaagctcga gaagatgacg gaagggatga agggcgtcga gcgcacgttc
     8701 gcgatccagg ccggccgcga gctgcgcgtg atcgtggacg cgaagaagat ctcggacgac
     8761 gagtgcctgt ggatgtcgaa ggacatcgcc aagcgcgtcg aagcggagct ccagtacccg
     8821 gggcagatca aaatcacggt cattcgcgag acgcgcgcgg tggacttcgc aaagtgaagc
     8881 tcctgttcgt cggtgacgtg gtcgggaaac ccgggcggcg ggccctcggt atgctcgaga
     8941 agctgatcgc gcgcgagcac gtcgacttca cggtcgtgaa catcgagaac gccgcgggcg
     9001 gcttcggcgt gacggccgag ctcttcgccg acatgtccaa gctcccgatc gacgtcttca
     9061 cgtccggaaa ccacatctgg gacaagaagg acttcgtgcc gctcctcgac gacctcgaga
     9121 acctgctgcg ccccgcgaac tacccgccgg gcaatccggg ccgcggctgg acgatccaga
     9181 cgacgcctgc cggcgttccc gtcggcgtcg tgaacctgca ggggcaggtc ttcatggcga
     9241 acacggattc gccgttccgc gtcggcgacg cggttctcgc cgaaatgcgc gccaagcggc
     9301 cggagatgaa ggtcgtcctg atcgacatcc acgccgaggc gacgtccgag aaacaggcga
     9361 tcgggtggtg gttcgacggg cgcgcgtcgc tcgtcatcgg cacgcacacg cacgtcccga
     9421 cggccgacgc ccggatcctg ccgaagggca cggcctacca gggcgacgcc gggatgacgg
     9481 gcgcctacga ggggatcatc gggttcgacc cgaagaacat cctcgagaag ttcctccacc
     9541 agacgccgcg ctcgatggag acggcgaccc gcgacgtgcg cctctgcggc gccatcgtgg
     9601 acgtggacga ggcgacgggg aaggcccgca cgatcacctc gatccaggag aagctggcca
     9661 cctgaccggc cgcgtcgtgg acatgcgcac gcggctcctc gtcctcgcgg tgttcgcggt
     9721 cacgttcgcg ttcgcggccg agccgcttct cacggggcgg ggcacggact tccgcgcgta
     9781 cctcgaggcc gcgaacgccc tgcgagccgg ccacgacccg tacgcgggcg tctatccgta
     9841 cctctacccg ccgctgcttg cggttctcct cgtcccgctc acgttcctgc cgcccggggc
     9901 ggccgcgtgg ctctgggcgg ccgcctcggc cgccgcactc gcggcgagcg ccgctctcgt
     9961 ggcccgcggc tcggcgcgca tcctcgttct agcgctcctc ttcgcgccgt tcgccgcgac
    10021 ccagtggaac ctccaggcga acgcgttcgt cctgcttctc ctcgtccttg cccgcgatcg
    10081 cctggaccgc gggttcgaag ggcgcggcgg ggcgctgctc ggtctttcga tcgctctcaa
    10141 gccgttcggc gtcctcgccg cctgcgccct cgctctcacc ggccggtggc gtgcggcact
    10201 cgccgcggga gcggcggcgg tcctcccgtt catcctcgtc gtcccgttcc tcgggggggc
    10261 gggagctgcg ggggcggccg gatcggtcaa gcggatcctc gcctcgtcct gggtcgagac
    10321 gtacggcgga aacgtctcgc tcaacggatc gctggaccgt gcgcttccgg agggcgccgg
    10381 ttcctcccgc caccgggcgg tggggctcgc ggtcgcgggg tggacggccg ccctcgtcgc
    10441 cgcggccgcc gccggcgcgg ctcggagcgg gaagcgcctg c
//
LOCUS       DAOMFY010000127        10240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_1998_length_10240_cov_7.318311, whole genome shotgun sequence.
ACCESSION   DAOMFY010000127 DAOMFY010000000
VERSION     DAOMFY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1998_length_10240_cov_7.318311"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..683
                     /locus_tag="PLB01_11205"
     CDS             48..683
                     /locus_tag="PLB01_11205"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQQ77914.1"
                     /translation="MKSKVAARRTTPGGAVLTVERPSGYADAALGESIAVSGVCLTVV
                     QPLDPGTLTFDVSPETLARTTLGGLAPGARVNLERALLPTDRLGGHVVAGHVDGTSEV
                     LAVAKAGDSWTFTFGLPAALARYAVEKGSIAVDGISLTIAALRAGSFDVAVIPHTFAE
                     TTLGDRRAGDRVNLEVDLLGKYVERLLAARLGDTDALARDERLRQLLAEGA"
     gene            680..1870
                     /gene="hemW"
                     /locus_tag="PLB01_11210"
     CDS             680..1870
                     /gene="hemW"
                     /locus_tag="PLB01_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQQ77915.1"
                     /translation="MTRGPADLPPGATSAPGAYVHVPFCAHRCTYCSFVALEGKREEE
                     DFFEGVEREIRSIAAAPAEGGSAAIRAFDSVYFGGGTPSYVDARRLGRLLGTFKEVFG
                     LSSDVEITAEANPDDLDAAKISGLQAIGINRLSVGVQSLVDSELVPLERRHDAEAARR
                     AVRKAVAAFGNVSADLMIGIPGQTRESLGQSLEGLLEAGVRHLSVYLLEIDKAPRLVA
                     LKRDRPALFADDDEMADRWLEVDERLEAAGLPRYELSNWARTGSESRHNLKYWTLVPV
                     LGFGVAAHSFDGRKRFANTGMLAEYLERTAAGALPVATFEETEDSDFLRMKEGLMIRL
                     RLAGGVPSYSFEEIRRTLPVASAQRLEDAFAAGLLEEISSPPRIRLTRKGVLLSNEVF
                     SALV"
     gene            complement(1867..3288)
                     /locus_tag="PLB01_11215"
     CDS             complement(1867..3288)
                     /locus_tag="PLB01_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQQ77916.1"
                     /translation="MGTMTKSGTTQPAYPRTLPRRLGLLDATTIVAGSMIGSGIFITS
                     ADISRQVRSGSLLVLVWVFTALVTVAGAWSYGKLAMAFPRAGGQYVYLREAWGDLGGF
                     LFGWAMLLVIQTGTIAAVAVAFAKYLGVLVPAVSSKHVFFKARFLSVTPLEIVAIALI
                     VLLTWWNTTSVENGARLQNVFTTAKVLSLVGLLAVCFTAGSGLATTNWAFPAAADLKM
                     PLIWAFAVATVGSLFSADAWNNVTFLGEEVRDAERNVPRALVYGTGLVTTLYVLANIG
                     YLNALPLSGIASAPEDRVATAAIGAVTGSAESGTPALIMAVVILVSTFGCLNGLVLSG
                     SRVLYAMSRDGLFLKSLAKVAPKSMIPVNALGAQALWASLLCLSGKYNDLLDYVIATV
                     LIFYIATIAGRWKLARTNPALAPKTLMDRVVPVLYVIATLYVTVALALYKPTFTVPGL
                     VIVALGIPAFYFFKSRQAPAPPA"
     gene            complement(3383..5818)
                     /locus_tag="PLB01_11220"
     CDS             complement(3383..5818)
                     /locus_tag="PLB01_11220"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQQ77917.1"
                     /translation="MTTSTRLVRTARAARGRAVAFLLALAALLADGVARADESELILP
                     DLGSVTFLGMNGRTLLMGGILVCLAGLAFGLAMYGKLKNLPVHKSMLEVSELIYETCK
                     TYLVTQGKFILILELFIGTIIAVYYGVLRHMEIGKVVVILLASLVGIAGSYGVAWFGI
                     RINTFANSRTAFASLKGKPFPCYSIPLAAGMSIGTMLISTELILMLGILLFIPGHLAG
                     PCFIGFAIGESLGASALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCTGD
                     NAGDSVGPTADGFETYGVTGVALISFILLAVPDAPTQVKLLVWLFAMRIMMIVASVAS
                     YWINDAIARAKYANADKMDFEAPLTSLVWITSLVSVAITFVVSYLLIPELGEGTLWWK
                     LGAVITCGTLAGAIIPELVKVFTSTKSGHVKEVVTASREGGASLNVLAGLTAGNFSAY
                     WMGLAIASLMGIGYAISTSFPSGLMLAPATFAFGLVAFGFLGMGPVTIAVDSYGPVTD
                     NAQSVYELSVIENVPNIKAEVKKDFGFDLSFDKAKHFLEENDGAGNTFKATAKPVLIG
                     TAVVGATTMIFSIIMLLTHGLKSDLDKLSILHSPFLLGLVMGGAVIYWFTGASTQAVS
                     TGAYRAVEFIKKNIKLDASVEKASVEDSKAVVAICTKYAQKGMFNIFLTIFFSTLAFA
                     CLEPYFFIGYLISIALFGLFQAIFMANAGGAWDNAKKLVEVDLKEKGTELHAATVVGD
                     TVGDPFKDTSSVAMNPVIKFTTLFGLLAVELAIGLSRGTSATFAAVFFAISLVFVYRS
                     FYGMRIESSPTAS"
     gene            complement(5913..6362)
                     /locus_tag="PLB01_11225"
     CDS             complement(5913..6362)
                     /locus_tag="PLB01_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQQ77918.1"
                     /translation="MSMGVGSGDGPKSEINVTPLVDVVLVLLIIFMVVQPLLQRGYDV
                     QVPPNAPATPPNTPPPTDQIIVSITANKEYYLNKEKVEAANIAIRLQEVLRNRASKIV
                     FFSAEDTVKYADVMTLMDIVRNSGSGDKKVNIGIVMDWVNPAAPATP"
     gene            complement(6373..6792)
                     /locus_tag="PLB01_11230"
     CDS             complement(6373..6792)
                     /locus_tag="PLB01_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQQ77919.1"
                     /translation="MGMDSFGGRQDMKSDINVTPLVDVCLVLLIIFMVVTPMLQKGKP
                     VMLPQTERPDKKPESDKELLISIQSDKTIFIDTKWFPDKEFAAKMKEIGERSANKDVL
                     VKADQRLNYGDVKNVMRMIKDGGFERVGLITEKKGDK"
     gene            complement(6835..7515)
                     /locus_tag="PLB01_11235"
     CDS             complement(6835..7515)
                     /locus_tag="PLB01_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQQ77920.1"
                     /translation="MDMSMMGLWNSMGPMAKSVFILLCALSVYSLAVSVERWMTFQKA
                     KQQSVKFALEVGQLLKQDKLREAIDLAKKYKNSHVAKVVSAGLLEFAYEAHGASAAGH
                     DSVAAAERAIERATMMTTADMKKGLGGLATIATTAPFIGLFGTVIGIINAFRGMAASG
                     AGGLGSVSAGISEALVTTALGLFVAIPAVWLYNLFLNKIERFQVEMSNSASELIDYFM
                     KRQAAKAA"
     gene            complement(7586..8320)
                     /locus_tag="PLB01_11240"
     CDS             complement(7586..8320)
                     /locus_tag="PLB01_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQQ77921.1"
                     /translation="MFENSLIESRKQHDAAKRWYTLPLSILIHVVVIGTAVGLSMWYI
                     EEIPEPPIPVQFYSQAAPPPPPPPPPPKAAAPKQQAKVEPTKPTTVTSPVVVPDTLPP
                     PTTAPEPADSGVEGGVEGGVEGGVAGGVMGGVLGGVKGGVIGGNVEEPLRVGGEVKEP
                     IEINRVKPQYPEAARKARMQGVCILEAIITKTGDVESVRVLRGLNPLLDNAAIRAVQQ
                     WKYKPATFNGRPVPVYLTVTVTFTLQ"
     gene            complement(8337..9230)
                     /locus_tag="PLB01_11245"
     CDS             complement(8337..9230)
                     /locus_tag="PLB01_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ77922.1"
                     /translation="MTTNRTGGRKGQRLAAAALVLLSFAAAGCDKFKSKQLIREGNAY
                     FKEQLYEDALKKYEQAQALDPEEVRLDKFVAMGNMALYNPGSTHPKDIAALTKAIEHF
                     KKYLAARPDDEKAAKFLVTTYMNAQKYDEAIGYFKDFIANHPKDAQAVQTVAMLYAKK
                     GDYENSIDWQKKRAALEPTNADVFYTMGVTAWQKSYDSPGNDVSPEHGLSPERRKEIL
                     DFGMSSLDKAIELNPDYFEAMLYKNLVYRQYARIEGDPAKVAELTAKATEWQTKALEV
                     RKKVQDKQKAEQASKNPLEAM"
     gene            complement(9303..9881)
                     /locus_tag="PLB01_11250"
     CDS             complement(9303..9881)
                     /locus_tag="PLB01_11250"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQQ77923.1"
                     /translation="MTLHVRRHLTAGILLIVPVAVTVAVLKFLFGLFDGWMQPFAEKA
                     FGRPIPGLGLLLTLLLVWITGVLSTNFAGKKLLELFDRLLENLPLVRTVYTASKQLVD
                     AVSPGGRRAFRKVVLVEFPKKGTFALGFVTGAGLGPLPPDMISVYVPTALNPTSGFLV
                     FARESDILDPRLSVEEGIKLVVSGGVVRPPGN"
     gene            9923..>10240
                     /locus_tag="PLB01_11255"
     CDS             9923..>10240
                     /locus_tag="PLB01_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77924.1"
                     /translation="MRTAPAGTVLTLLAAVLGGWAAAPRLAAQEKPGAGSAPFIAREG
                     VSVTNLDVVVTDGKGNRVTGLKKEDFIVVEDGVELPVSNFTAIEQGRFLLPPEEPAAS
                     AAAS"
BASE COUNT         1717 a         3221 c         3548 g         1754 t
ORIGIN      
        1 ggcctcacga ggcgtaggat cgggtcgtat tcatgtgacg cgactccgtg aagtcgaagg
       61 tcgccgcccg ccgcacgact ccgggcggcg cggtcctcac cgtcgagcgg ccctccgggt
      121 acgcggacgc ggcgctcgga gagagcatcg cggtgtcggg ggtgtgtctc acggtggtcc
      181 agccgctcga ccccgggacg ctcacgttcg acgtgtcgcc cgagacgctg gcgcgcacga
      241 cgctcggcgg cctggcgccg ggtgcgcgcg tgaacctcga gagggccctc ctgccgacgg
      301 accgcctcgg cgggcacgtc gtcgcggggc acgtcgacgg gacgtccgag gttctcgcgg
      361 tcgcgaaggc cggcgactcg tggacgttca cgttcggcct tcccgccgct ctcgcgcgct
      421 acgcggtcga gaagggctcg atcgcggtcg acggcatctc gctcacgatc gcggcgctgc
      481 gagcgggctc gttcgacgtc gccgtcatcc cgcacacgtt cgccgagacg acgctcgggg
      541 accgcagggc cggcgaccgc gtgaacctcg aggtcgacct cctcggcaag tacgtcgagc
      601 gcctgctcgc ggcgcgcctc ggcgacacgg acgccctcgc gcgcgacgag cgcctcaggc
      661 agctcctcgc cgaaggcgcg tgacgcgcgg cccggccgac cttccgccgg gcgcgacgtc
      721 cgcgcccggc gcgtacgtcc acgtgccgtt ctgcgcgcat cgctgcacgt attgctcctt
      781 cgttgctctc gaggggaaga gagaagaaga agatttcttt gaaggggtag agagggagat
      841 ccgctcgatc gcggctgccc cggcggaggg cggctcggct gcgattcgcg cctttgattc
      901 ggtctacttc ggagggggaa cgccgtcgta cgtcgacgcg cgccgcctcg gccgcctgct
      961 cgggaccttc aaagaagtct tcggtctctc ttcggacgtc gagatcacgg cggaggccaa
     1021 cccggacgac ctcgacgcgg cgaagatctc cggtcttcaa gccattggaa tcaatcgcct
     1081 gtccgtcggc gtccagtcgc tcgtcgactc cgagctcgtc cctctcgagc gccggcacga
     1141 cgccgaggcg gcccggaggg ccgtccggaa ggccgtggcg gccttcggaa acgtctccgc
     1201 ggacctcatg atcggaatcc cgggccagac ccgggagagc ctcgggcaga gcctcgaagg
     1261 gctcctggag gccggcgtcc gccacctctc ggtctaccta ctcgagatcg acaaggcgcc
     1321 ccgcctcgtg gccctgaagc gcgaccggcc cgcgctcttc gcggacgacg acgagatggc
     1381 cgaccgctgg ctcgaggtgg acgagcggct ggaagctgcc ggcctcccgc gctacgagct
     1441 ctcgaactgg gcgcggaccg gctccgagag ccgccacaac ctcaaatact ggacgctggt
     1501 gccggtcctc gggttcggcg tcgccgcgca ctcgttcgac ggaagaaagc gctttgcaaa
     1561 tacgggaatg ctcgccgagt atctggagcg caccgccgcg ggcgccttgc ccgtcgcgac
     1621 gttcgaagag accgaagatt cggattttct tagaatgaaa gaggggctga tgattcgcct
     1681 gcgccttgca ggcggggtcc cctcttactc attcgaagaa atccgaagga cgcttcccgt
     1741 cgcctcggca cagcgactcg aggacgcgtt cgcggcaggc ttactcgaag aaatctcttc
     1801 ccctccccgc atccgcctca cgcgcaaggg cgtgctgctg tcgaacgagg tcttctcggc
     1861 cctcgtctag gccggcgggg ccggcgcctg ccgggacttg aagaagtaga aggccgggat
     1921 cccgagcgcc acgatcacga gtccggggac cgtgaacgtc ggcttgtaga gggcgagcgc
     1981 gaccgtgacg tagagcgtcg cgatcacgta gaggaccggg acgacccggt ccatgagcgt
     2041 cttcggcgcg agggccggat tcgtacgggc gagcttccag cggccggcga tcgtcgcgat
     2101 gtagaagatg aggacggtcg cgatgacgta gtcgaggagg tcgttgtact tgcccgaaag
     2161 gcagagaagg gaagcccaga gcgcctgcgc gccgagcgcg ttgaccggga tcatcgactt
     2221 gggcgcgacc ttcgcgagcg acttgaggaa gagcccgtca cgcgacatcg cgtagaggac
     2281 gcgcgatccg gacaggacga gcccgttcag gcagccgaac gtcgacacga ggatgaccac
     2341 cgccatgatg agcgccggag tgcccgattc cgcgctgccc gtcacggctc cgatggccgc
     2401 cgtggcgacg cggtcctcgg gcgccgaggc gatgcccgag agcgggaggg cgttgaggta
     2461 gccgatgttc gcgaggacgt agagcgtcgt cacgaggccg gtgccgtaga cgagcgcgcg
     2521 cgggacgttg cgctcggcgt cgcggacctc ctccccgagg aacgtcacgt tgttccacgc
     2581 gtccgcggag aagagggagc cgaccgtcgc gaccgcgaac gcccagatca gaggcatctt
     2641 gaggtcggcc gccgcgggaa aggcccagtt cgtggtcgcg aggccgctcc cggccgtgaa
     2701 gcagacggcg aggagaccga cgagggagag gaccttggcc gtcgtgaaca cgttctggag
     2761 ccgggcgccg ttctcgacgc tcgtcgtgtt ccaccacgtg aggagaacga tgagcgcgat
     2821 cgcgacgatc tcgaggggcg tcacgctcag gaagcgggct ttgaagaaca cgtgcttgct
     2881 cgacacggcc gggaccagga cgccgaggta cttcgcgaag gcgaccgcca cggcggcgat
     2941 cgtgcccgtc tggatgacga ggagcatcgc ccagccgaac aggaagcccc cgaggtcgcc
     3001 ccatgcctcc cggaggtaga cgtactggcc gccggccctc gggaaggcca tggcgagctt
     3061 cccgtagctc caggcgcctg cgaccgtgac gagagccgtg aagacccaga cgagaacgag
     3121 gagggacccg ctccgcacct gacgggagat gtccgcggac gtgatgaaga tgcccgagcc
     3181 gatcatcgag cccgcgacga tcgtggtcgc gtcgaggagt ccgagacggc gggggagcgt
     3241 ccgtggatag gccggctgcg tcgtgccgga cttggtcatc gtgcccatcg accctccttg
     3301 ggttggccgg gattctaccc acaccgcggg aagaaaaagg gccgcccgaa ggcggccctc
     3361 gatgacgcgg agttccgagg ggtcaggagg cggtcggcga actctcgatg cgcattccgt
     3421 agaaggaccg gtagacgaac acgagcgaga tcgcgaagaa gaccgcggcg aacgtggcgc
     3481 tcgtcccgcg cgacagtccg atcgcgagct ccacggcgag gaggccgaag agcgtcgtga
     3541 acttgatgac cgggttcatg gccacggagg acgtgtcctt gaaggggtcg ccgaccgtgt
     3601 cgccgacgac ggtcgccgca tgcagctcgg tgcccttctc cttgaggtcg acctcgacga
     3661 gcttcttggc gttgtcccag gcgccgccgg cgttcgccat gaagatcgcc tggaagaggc
     3721 cgaagagcgc gatcgagatg aggtagccga tgaagaaata cggctcgagg cacgcgaacg
     3781 cgagcgtcga gaagaagatg gtgaggaaga tgttgaacat gcccttctgc gcgtacttgg
     3841 tgcagatggc gacgacggcc ttgctgtcct cgacggaggc cttttcgacc gaggcgtcga
     3901 gcttgatgtt tttcttgatg aactcgacgg cacggtaggc gccggtggag accgcctggg
     3961 tggaggcgcc cgtgaaccag tagatgaccg cccctcccat gacgaggccg agcaggaacg
     4021 gcgaatgcag gatcgagagc ttgtcgaggt cgctcttgag gccgtgggtc aggagcatga
     4081 tgatcgagaa gatcatcgtc gtcgcgccga cgacggccgt cccgatgagc acgggcttcg
     4141 cggtcgcctt gaacgtgttg cccgcgccgt cgttctcctc gaggaagtgc ttcgccttgt
     4201 cgaagctgag gtcgaagccg aagtccttct tgacctcggc cttgatgttc gggacgttct
     4261 cgatgaccga gagctcgtac acggactggg cgttgtcggt gacggggccg taggagtcga
     4321 ccgcgatcgt gacggggccc attccgagga agccgaacgc gacgaggccg aacgcgaagg
     4381 tcgccggggc gagcatgagg cccgagggga acgaggtcga gatcgcgtag ccgatgccca
     4441 tcagggacgc gatggcgagg cccatccagt aggccgagaa attgccggcg gtgaggccgg
     4501 cgaggacgtt cagggacgcg ccgccctcgc gggaggcggt cacgacttcc ttgacgtggc
     4561 cggacttcgt ggacgtgaac accttgacga gctccgggat gatcgcgccg gcgagcgtgc
     4621 cgcacgtgat gaccgccccg agcttccacc agagcgtccc ctcgccgagc tcggggatca
     4681 ggaggtagga gacgacgaac gtgatggcga ccgagacgag cgacgtgatc cagacgagcg
     4741 aggtcagggg ggcctcgaag tccatcttgt cggcgttcgc gtacttcgcc ctggcgattg
     4801 cgtcgttgat ccagtacgag gcgacgctcg cgacgatcat catgatgcgc atcgcgaaga
     4861 gccagacgag gagcttgacc tgggtcggcg cgtcggggac cgcgaggagg atgaaggaga
     4921 tgagcgcgac gccggtgacg ccgtaggtct cgaagccgtc ggccgtcggc ccgacggagt
     4981 cgcccgcgtt gtcgcccgtg cagtccgcga tgacgccggg gttgcgggcg tcgtcttcct
     5041 tgatcttgaa gacgatcttc atgaggtccg acccgatgtc ggcgatcttc gtgaagatgc
     5101 cgcccgcgat gcggagggcc gaggcgccga gcgactcgcc gatggcaaac ccgatgaagc
     5161 aggggcccgc gaggtggccc gggatgaaga gaaggatccc gagcatgagg atgagctcgg
     5221 tcgagatgag catcgtcccg atcgacatgc ctgccgcgag ggggatcgaa tagcagggga
     5281 agggcttgcc cttgaggctc gcgaacgcgg tgcgcgagtt cgcgaacgtg ttgatccgga
     5341 tgccgaacca ggcgaccccg tagctcccgg cgatgccgac gaggctcgcg aggaggatca
     5401 cgaccacctt gccgatctcc atgtgacgga ggacgccgta gtagacggcg atgatcgtcc
     5461 cgatgaagag ctcgaggatc aggatgaact ttccctgggt gacgaggtag gtcttgcacg
     5521 tctcgtaaat gagctcggag acctcgagca tcgacttgtg gacgggcagg ttcttcagct
     5581 tcccgtacat cgcgaggccg aaggcgagac cggcgaggca gacgaggatg ccgcccatga
     5641 ggagcgtccg gccgttcatt ccgaggaacg tcacggaccc caggtcgggg aggatcagct
     5701 ccgattcgtc ggcgcgtgcg acgccgtcgg cgaggagcgc ggccagggcg agcagaaacg
     5761 cgacggcgcg cccccgggcg gcgcgtgccg tgcggaccag gcgggtggag gtggtcatcg
     5821 gttcagatcc tttcttgctg cggcaaacga acaacgtccc cggggtgagc cggggacgaa
     5881 gagcggaagc tttgctggcg gggtcggaag tgtcacggcg tcgcgggcgc ggcggggttc
     5941 acccagtcca tcacgatgcc gatgttgacc ttcttgtcgc cggaaccgga gttgcggacg
     6001 atgtccatca gggtcatgac gtccgcgtac ttgacggtgt cctccgcgga gaagaagacg
     6061 atcttcgacg cgcggtttcg gaggacctcc tggaggcgga tcgcgatgtt cgcggcctcg
     6121 accttctcct tgttgaggta gtactccttg ttcgcggtga tcgacacgat gatctggtcc
     6181 gtcggcggcg gcgtgttcgg aggcgtcgcc ggcgcgttcg ggggcacctg gacgtcgtag
     6241 ccgcgctgga ggagcggctg gacgaccatg aaaatgatca ggaggacgag aacgacgtcg
     6301 acgagcggag tgacgttgat ctccgacttc gggccatccc cgcttccgac tcccatgctc
     6361 atggatgact ccttacttgt cgcccttctt ctcggtgatg agtccgacgc gctcgaatcc
     6421 gccgtccttg atcatgcgca tgacgttctt gacgtcgccg tagttcaggc gctgatcggc
     6481 cttgacgagc acgtccttgt tcgcggaacg ctctccgatt tccttcatct tcgccgcgaa
     6541 ctccttgtcg ggaaaccact tcgtgtcgat gaagatggtc ttgtccgact ggatcgagat
     6601 gaggagctcc ttgtcggact ccggcttctt gtccgggcgc tccgtctgcg gaagcatgac
     6661 gggcttgccc ttctgcagca tcggcgtgac gaccatgaag atgatgagga ggacgaggca
     6721 cacgtcgacg agcggcgtca cgttgatgtc gctcttcatg tcctgtcggc cgccgaagct
     6781 atccattccc atcgaactcg ctccttcgtt tcctgtggac ggacccggga ttcctcaggc
     6841 ggccttcgcg gcctggcgct tcatgaagta gtcgatcagc tcggaggccg agttcgacat
     6901 ctccacctgg aagcgctcga tcttgttgag gaagaggttg tagagccaca cggccgggat
     6961 ggcgacgaag aggccgagcg ccgtcgtgac gagggcctcg gagataccgg ccgagactga
     7021 gccgaggccg ccggcgccgg acgccgccat gccgcggaac gcgttgatga tgccgatgac
     7081 ggtgccgaag aggccgatga acggcgccgt cgtcgcgatc gtcgcgaggc cgccgaggcc
     7141 cttcttcatg tcggccgtcg tcatcatcgt ggcgcgctcg atcgcgcgct cagcggccgc
     7201 gacgctgtcg tggcccgccg ccgaggctcc gtgggcctcg tacgcgaact cgagaagtcc
     7261 cgccgagacg accttggcga catggctgtt cttgtacttc ttggcgaggt cgatggcttc
     7321 cctcagcttg tcctgcttga ggagctggcc gacctcgagc gcgaacttga ccgactgctg
     7381 cttggccttc tggaacgtca tccaccgctc gaccgagacc gcgagagagt agaccgaaag
     7441 ggcgcagagc aggatgaaga cgctcttcgc catgggcccc atcgagttcc aaaggcccat
     7501 catgctcatg tccatttgat tcgtcctcct taacgtttcg ccgaatttga ttctacgtcg
     7561 tcgtgttctg tcattcgtgt ggcggtcact ggagcgtgaa cgtgaccgtg accgtcaggt
     7621 aaaccggaac cgggcgcccg ttgaaggtcg ccggcttgta cttccactgc tggaccgcgc
     7681 ggatcgcggc gttgtcgaga agcgggttca ggccgcgcag cacgcgcacg gactcgacgt
     7741 cacccgtctt cgtgatgatg gcctcgagga tgcagacgcc ctgcatgcgc gccttgcggg
     7801 ccgcctcggg atactgcggc ttgacgcggt tgatctcgat cggctccttg acctcgccgc
     7861 cgacgcggag gggctcttcg acgttgccgc cgatgacgcc tcccttgacg ccgccgagga
     7921 cgcctcccat cacgccgccc gcgacgccgc cttcgacgcc gccctcgacg ccgccctcga
     7981 cgccggaatc cgcgggttca ggcgcggtcg tcggcggggg gagcgtgtcg ggcacgacca
     8041 cgggggacgt gaccgtcgtg ggcttcgtcg gctcgacctt cgcctgctgt ttcggagccg
     8101 cggccttcgg gggcggcggc gggggcggcg gcgggggagc cgcctgcgag tagaactgca
     8161 ccgggatcgg cggctccggg atctcctcga tgtaccacat cgagaggccc accgccgtgc
     8221 cgatcacgac cacgtgaatc aggatcgaga gcggaagcgt gtaccagcgc ttcgccgcgt
     8281 cgtgctgctt ccgcgattcg atgagggaat tttcgaacat tcgtcctccc gcgtccctac
     8341 atcgcctcga gcgggttctt gctcgcctgc tcggccttct gcttgtcctg gaccttcttg
     8401 cggacctcga gcgccttcgt ctgccactcg gtggccttcg ccgtcagctc ggcgaccttg
     8461 gccgggtcgc cctcgatgcg ggcgtactgg cggtagacga ggttcttgta gagcatggcc
     8521 tcgaagtagt cgggattgag ctcgatcgcc ttgtccaggg agctcatgcc gaagtcgagg
     8581 atctccttgc gcctctccgg cgagagcccg tgctcggggg agacgtcgtt gccgggcgag
     8641 tcgtaggatt tctgccaggc cgtgacgccc atcgtgtaga agacgtcggc gttcgtgggc
     8701 tcgagggccg ctcgcttctt ctgccagtcg atcgagttct cgtagtcgcc cttcttcgcg
     8761 tagagcatcg cgacggtctg gacggcctgc gcgtccttcg ggtggttcgc gatgaagtcc
     8821 ttgaagtacc cgatcgcctc gtcgtacttc tgggcgttca tgtacgtcgt gacgaggaac
     8881 ttcgcggcct tctcgtcgtc ggggcgcgcg gcgaggtact tcttgaagtg ctcgatcgct
     8941 ttcgtgaggg cggcgatgtc cttggggtgc gtcgagcccg ggttgtagag ggccatgttc
     9001 cccatggcga cgaacttgtc gaggcgcact tcttcggggt cgagcgcctg ggcctgctcg
     9061 tacttcttca gggcgtcttc gtagagctgc tccttgaagt aggcgttccc ttcccggatg
     9121 agctgcttgc tcttgaactt gtcgcagccg gccgccgcga aagagaggag gacgagcgcc
     9181 gctgcggcga gccgctgccc cttccttccg ccggtccgat tcgtcgtcat gacccgttca
     9241 ctcctttcga aaaacaccca cccgcccgtg caggctccga gttggacccc gccggtgcgt
     9301 cgtcagttcc cgggcggcct caccacgccg cccgaaacga cgagcttgat cccctcctca
     9361 accgagaggc gagggtcgag aatatcgctt tcccgggcaa acaccagaaa tccgctcgtc
     9421 gggttcaggg ccgtcggcac gtaaacggaa atcatgtccg gggggagggg gccgaggccg
     9481 gcgcctgtga cgaaacccaa cgcgaaggtt cccttcttcg gaaattcgac aagcaccact
     9541 ttgcggaaag cgcggcgccc gcccggagag acggcatcca cgagctgctt cgaggccgtg
     9601 tagaccgtcc tgacgagggg gagattttcc aagaggcgat cgaaaagttc aaggagtttc
     9661 ttcccggcga agttcgtcga caaaacgccc gtaatccaaa cgagcagcaa ggttaagagc
     9721 agccccaggc ccggaatcgg acgcccgaag gccttctcgg cgaacggctg catccacccg
     9781 tcgaagagtc cgaaaaggaa cttcaggacg gcgactgtca cggcgaccgg gacgatgagg
     9841 aggatgccgg ccgtgaggtg ccggcgcacg tggagcgtca tcgggaagcg cattctatgc
     9901 gggccgtaca atcccggaaa tgatgcgaac tgctcctgcc gggaccgtcc tcaccctcct
     9961 cgccgccgtc ctcggcggat gggctgccgc gccgcgcctc gccgcccagg agaagcccgg
    10021 cgccggatcg gcgccgttca tcgcacgcga aggcgtctcg gtgaccaacc tcgacgtcgt
    10081 cgtgaccgac gggaagggca accgcgtgac gggcctgaag aaggaagact tcatcgtcgt
    10141 cgaggacggg gtcgagctgc cggtctcgaa cttcaccgcg atcgaacagg gccgcttcct
    10201 ccttcccccc gaagagccgg cggcctccgc ggcggcgtcg
//
LOCUS       DAOMFY010000128        10210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2008_length_10210_cov_7.149385, whole genome shotgun sequence.
ACCESSION   DAOMFY010000128 DAOMFY010000000
VERSION     DAOMFY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2008_length_10210_cov_7.149385"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLB01_11260"
     CDS             complement(<1..253)
                     /locus_tag="PLB01_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77925.1"
                     /translation="MRRFAAHPAAALLVAALLAPIFGAGAVLADDLRDMAAGNTGLAD
                     NPETAGPDLFAPRLWGGDIPDMPSLLVLDAPSDAAPADAP"
     gene            321..1712
                     /locus_tag="PLB01_11265"
     CDS             321..1712
                     /locus_tag="PLB01_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQQ77926.1"
                     /translation="MSATPAAEGGLKREIGLWQGTALNVIDMVGIGPFVTLPLILAAM
                     GGGRALLAWILGALLAISDGLVTAELSASLPRAGGSYAFLREAYGPERWGKLVSFLFL
                     FQIVFSAPLSMASGAIGFARYLRFVFPGIPEGWEAPAASLLCVAVTLLLMRRIGAIGK
                     FSIVLWVGVLATLGIVIVLGLPHMKLEALRFWEAPVVSAPGIPPPGVLGGLGAALILA
                     VYDYLGYYNICYLAEEIVEPTKTIPRVIVISILAVAVLYILMNVCIISVLPMDQAMSS
                     KSTVADFVQLLAGRPVARAVTGLILWTAFASVFSLTLGYSRILWAAARDGNFFGVFAR
                     LHPTEGYPFVAIAALGGAAAIFSFLSLKVVVSAIISIRALIPFVAQVAGALVLRRTQP
                     GLKRPFRMWLYPLPALVALGLWGFVLVSPEKGFKAAGLLVIVAGVSAFLVRSRLAREW
                     PFAGAAGQESAAP"
     gene            1709..2680
                     /gene="pfkA"
                     /locus_tag="PLB01_11270"
     CDS             1709..2680
                     /gene="pfkA"
                     /locus_tag="PLB01_11270"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQQ77927.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRTGIARGASVIGARHGFSGL
                     IAGDLVPLGARAVGGIIQQGGTILGSARCDEFRTPEGRAGAIEVLRRNEVEGLVVIGG
                     SGSQSGAHELQKLGFPVVGVASTIDNDLYGSDITIGVDTALNIALEAIDRLKTTASSH
                     ERAFLVEVMGRDCGYLALMAGIAGGAESVVVPEVPVDPEELARELDASRERGKKHAII
                     VVAEGASYDAEGLAHYFREHKKRLGFDLRVTILGHVQRGGAPGAYDRLLATRLGFAAV
                     QSLMEGAGGVLMGLRDGRITATPLHAVAGAKKPLDPSLFELLRTMAK"
     gene            2719..2794
                     /locus_tag="PLB01_11275"
     tRNA            2719..2794
                     /locus_tag="PLB01_11275"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2752..2754,aa:Cys,seq:gca)
     gene            complement(2761..3465)
                     /locus_tag="PLB01_11280"
     CDS             complement(2761..3465)
                     /locus_tag="PLB01_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQQ77928.1"
                     /translation="MRSSALRIDDALDLIADAQAAACGMDPRHRIVYWNESATALLGW
                     TSGEVLGKKCYDVFAGRDVFGNICCFRDCGVAVASSRGEAAAPFVMDVRTREGTKARL
                     VTRTIALPSPGAAYRTLVHVFENGDGAVLDALLARVTSVVAPPAEPVFGPPEERITLS
                     PREQQILELLAGGYGSINIAARLGLSHATVRNHVQHLLRRLGVHSQVEAVSLAFRRGM
                     LGRDEAGGDGRSRTDE"
     gene            3541..3628
                     /locus_tag="PLB01_11285"
     tRNA            3541..3628
                     /locus_tag="PLB01_11285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3574..3576,aa:Leu,seq:taa)
     gene            3698..4258
                     /locus_tag="PLB01_11290"
     CDS             3698..4258
                     /locus_tag="PLB01_11290"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HQQ77929.1"
                     /translation="MNLILMSQPGEITALLRELRSGDRTAGDRLIEKAYPELHRIAQA
                     HFRHERPGHLLQPTALVNEAWQKFVALDAVDLRDRAHFLSLCSRLMRQILVDHARRRA
                     THERAVVSMSIDDRIEENMLDLDLALSKLEKAHPRAGRVIELRYFGGMSNEEVAQCLE
                     VSPATVKRDWLAARAWLYGQLRGNSA"
     gene            4255..5985
                     /locus_tag="PLB01_11295"
     CDS             4255..5985
                     /locus_tag="PLB01_11295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ77930.1"
                     /translation="MIGRRFEDLSGRLAEALESADPEAHVAASGDPELVELFAWHRRA
                     GDFLEGGGAKRENPWVGRHIAGRYLLRSVLGRGGSGVVFEACDEQVAGRRVVVKLLHD
                     FWSSEDWMRRRFREEAGVLARLDHPGIVSLIDAGEIEDGRLFLALPFHEGRTLREALA
                     AGPLDAPFAARLLREIGEAVGYAHAEGVLHRDLKPENILLVRRADGEHPLLIDFGIAQ
                     VGDAGGPPETTTHLMGSAVYMAPEHLMGKAEAASDVYALGVIAWEMLAGARPFDSASP
                     FALPELQRKGVGDTFYRLRPDLGTGVGKLLARALAYEAVRRPAPVTAFTGEIADALQA
                     GAIDSWLARLWVVRRSRRWMLAGGATAVMGAAAGGWWLRDRLTPLDAEERVIEFPRGA
                     TPDMAGFSIRRELTERAIRDFPSGAVTAMRYLTADQGTLYRRLSARQKEWAFRRGWKL
                     SALCRPESGQAGIAVENGSVAPAFVAVLNSTRDRVELIAVTQIRTGIAGLRAPLSLPP
                     APRLVQLEMAYDPASARATVSVDGQVLVRDYAGHTEYRSDPSVFFALSRTDETMASAV
                     FGGLRFEILG"
     gene            6077..8113
                     /locus_tag="PLB01_11300"
     CDS             6077..8113
                     /locus_tag="PLB01_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77931.1"
                     /translation="MRLAAGLLVTFGILVTGAASAAGTERDATDHTALVAQWQAPPYW
                     LPPARGAEASASGRQPLAGGPTALPFIALFPCRLVDTRAPAFPAPLGGGFLPPATVRS
                     YTLTGVCNVPPGAQAISLNVTVVNPTGPGFITLYPEGGAFPPVSTLNYLGHDVIVNAA
                     VVPLSATGGISMALGVSGGDVILDTNGYYATVPSVTSVNTLAGDLTLSAGSNVTITPS
                     GNTLTIASTGGGGGTVTSVGTGSGLTGGPITTTGTVSVAPLGITTGLLANNAVTSAKL
                     APGSVGLAQIDPTQVQGRVASGCPDGLSIQSINQDGSVACLASSPQAGFLISTIDSVG
                     DVGQYTSITTGWDGLGLISYYDATNGDLKVAHCSNPLCTTATITTLDSAGDVGLTSSI
                     TIGADSLGLISYYDATNGDLKVAHCSNLSCTSATITTLDSAGDVGLHSSITVAADGAA
                     LISYFDATNGGLKVAHCVNLSCTSAIKSTIDTGGVGNHSSIAIGADARGIISYYDATN
                     GDLKVAHCSNAACTSATVTTLDSAGDVGLFTSITAGGDGSPAISYYDNTNGRLKAASC
                     SDFLCSTASIFTIDGSGDVGRFGSSLTIGPDGFGLVSYLDLTNFVLKVAHCGSAGCTA
                     TVVDNSSPAHVGRYSSLTIGPDGFALISYYDFTNQNLKVAHCSNNLCLPWVRRR"
     gene            8143..8853
                     /gene="rnc"
                     /locus_tag="PLB01_11305"
     CDS             8143..8853
                     /gene="rnc"
                     /locus_tag="PLB01_11305"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQQ77932.1"
                     /translation="MENDEADGLDAVERALGHAFRDRRLLVRALTHSSYANDDPDAED
                     NETLEFLGDSILNYLVSEMLFDAFPLEAEGTLSKARSVLVSENHFAYLARRMDLGSAL
                     RLSPGEERAGGRNRDARLADAFEAVFAALALDAGLDEARALARRLFAEDIAALDLGGL
                     HRRDPKTALQERAQGEGLGLPLYRLVEESGPAHERRFVYEVTYGEGERATGAGSSKKE
                     AQTAAAEAMLRKREEGIS"
     gene            complement(8941..9999)
                     /locus_tag="PLB01_11310"
     CDS             complement(8941..9999)
                     /locus_tag="PLB01_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQQ77933.1"
                     /translation="MAGGSTEESGGLAGKSLPLLLGLVFVSGLALNLTPCVYPLIPIT
                     LGFFSRQSGGRKGGTFGLAVAYVLGMSVTYSALGVFAALSGSLFGAWLQKPAVIIGIA
                     AIVVALALSMFGLYEIQAPHFVTDRTGSQTGALGALTMGLFVGFVAAPCIGPFVLSLL
                     TYVAAKGSAPLGFALFFTLAMGLGLPYLVLGTVSGSLKALPRSGEWMTAVRKVFGFAL
                     LALAVYFLRPLLAPRVYEIGVALPLLAGGIWFLFFEKTGASVRGFAAVKVAVAVGLLA
                     AGAAFALPRKAGPELKFAPYSDAAVAAAKAAGKPVMIDFYADWCLPCKELDHKTFTDA
                     RVQKALEGWVLLKADLTR"
     gene            9956..>10210
                     /locus_tag="PLB01_11315"
     CDS             9956..>10210
                     /locus_tag="PLB01_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77934.1"
                     /translation="MPARPPLSSVEPPAIRLRNCSGGSNPTTRPPRSFPSGPRKRIVG
                     TPTTFHASRRAAVPPGGPPDGALPSAPASAHQPSGCRASRR"
BASE COUNT         1515 a         3552 c         3582 g         1561 t
ORIGIN      
        1 ggggcgcgtc cgcgggcgcc gcgtcggagg gcgcgtcgag gacgaggaga gagggcatgt
       61 cggggatgtc cccgccccag aggcgcggcg caaagagatc cggccccgcc gtctcgggat
      121 tgtccgcgag gccggtattg ccggcggcca tgtcccggag gtcatcggcc agcacggcac
      181 cagccccgaa tatcggggcg aggagggccg cgacgaggag cgcggcggct gggtgggcgg
      241 cgaaacggcg catgacggtt cgggcgcggg atcctacgca attcgcgcgc ccggccgcgg
      301 ccgggttact gtcccggacc atgagtgcaa ccccggcggc tgaaggcggg ttgaagaggg
      361 aaatcggcct ctggcagggc accgccctga acgtgatcga catggtcggg atcggtccgt
      421 tcgtgacgct tccgctgatt ctcgcggcga tgggcggcgg gcgggcgctc ctcgcgtgga
      481 tcctcggcgc ccttctcgcg atctcggacg gcctcgtgac ggccgagctt tcggcctcgc
      541 tgccccgggc cggcgggtcg tacgcgttcc tgcgcgaggc gtacggcccc gagcgctggg
      601 gcaagctcgt ctcgttcctc ttcctcttcc agatcgtctt ctccgcgccg ctctcgatgg
      661 cgtcgggcgc gatcggcttc gcccggtacc tgcggttcgt attccccggg attccggagg
      721 gctgggaggc gcccgcggcg tccctcctgt gcgtcgcggt gacgctgctc ctcatgcgaa
      781 ggatcggggc gatcgggaag ttctcgatcg tgctctgggt cggcgtcctc gcgacgctcg
      841 ggatcgtgat cgtcctcggc ctgccgcaca tgaagctgga ggcgctgcgc ttctgggagg
      901 caccggtcgt ctccgcgccc ggaatccctc cgcccggcgt cctcggcggg ctcggagccg
      961 cgctcatcct ggcggtctac gactacctcg gctactacaa catctgctac ctcgccgagg
     1021 agatcgtcga gcccacgaag acgatccccc gcgtcatcgt catttcgatc ctcgcggtcg
     1081 cggtcctcta catcctcatg aacgtctgca tcatctcggt cctcccgatg gaccaggcga
     1141 tgtcctcgaa gtccacggtc gcggacttcg tccagctgct cgcggggcgg cccgtcgcgc
     1201 gggccgtgac aggcctgatc ctgtggacgg cgttcgcgtc ggtcttctcg ctcacactcg
     1261 ggtactcgcg catcctgtgg gccgcggcgc gcgacggcaa cttcttcggg gtcttcgccc
     1321 gcctgcaccc gacggagggc tacccgttcg tcgcgatcgc ggcgctgggc ggggccgcgg
     1381 cgatcttctc gttcctgagc ctgaaggtcg tcgtctcggc gatcatctcg atccgggccc
     1441 tcatcccgtt cgtcgcgcag gtggccggcg cgctcgtcct ccgccggacc cagcccggcc
     1501 tcaagcgccc gttccggatg tggctctacc cgctgcccgc gctcgtcgcc cttgggctgt
     1561 gggggttcgt cctcgtctcg cccgagaaag ggttcaaggc ggcgggcctt ctcgtcatcg
     1621 tggcgggcgt ctcggccttc ctcgtgagat cccgtctcgc gcgagaatgg ccgttcgccg
     1681 gagccgccgg ccaggagtcc gccgcgccat gaagcgcatc gccgtcctca cgagcggggg
     1741 agacgccccg ggcatgaacg ccgcgatccg ggccgtcgtg cggaccggga tcgcgcgggg
     1801 ggcgagcgtg atcggcgcgc ggcacgggtt ctcgggcctg atcgccggag acctcgtccc
     1861 gctcggcgcg cgggcggtcg gcggcatcat ccagcagggc ggcacgatcc tcggcagcgc
     1921 ccgctgcgac gaattccgga ctcccgaggg gcgcgcggga gcgatcgagg tcctgcgccg
     1981 gaacgaggtc gagggcctcg tcgtgatcgg cggcagcggc tcgcagtcgg gcgcgcacga
     2041 gctgcagaag ctgggattcc cggtggtcgg cgtcgcgtcc accatcgaca acgacctcta
     2101 cggctcggac atcacgatcg gcgtcgacac ggccctcaac atcgcgctcg aggcgatcga
     2161 ccggctcaag acgacggcgt cctcgcacga gcgggcgttt ctcgtcgagg tcatgggccg
     2221 cgactgcggg tacctcgcgc tgatggccgg catcgcgggc ggcgcggagt ccgtcgtcgt
     2281 gccggaagtg ccggtcgacc cggaggagct cgcgcgcgag ctcgacgcgt cgcgcgagcg
     2341 gggcaagaag cacgcgatca tcgtcgtcgc cgaaggggcc tcgtacgacg ccgagggcct
     2401 cgcgcactat ttccgggagc acaagaagcg gctcgggttc gacctgaggg tcacgatcct
     2461 cggccacgtc cagcgcggcg gggcgcccgg agcctacgac cggcttctcg cgacgcgcct
     2521 cgggtttgcc gcggttcaga gcctcatgga gggggcaggc ggcgtcctga tgggcctccg
     2581 ggacggccgg atcaccgcga ccccgctgca cgcggtggcc ggggcgaaga agcccctcga
     2641 cccgtctctc ttcgaactcc tccggacgat ggcgaaatag cgcccgtccc ggttaagatc
     2701 cgcgtccggc tctgggacgg cgacgtaccc aagtggttaa gggaaggctc tgcaaaagcc
     2761 tcattcgtcg gttcgactcc gaccgtcgcc tccagcttcg tcccggccca gcatcccccg
     2821 cctgaaggcc agcgacaccg cctcgacctg actgtgcacg cccagcctgc ggagcaggtg
     2881 ctgaacgtgg ttgcggaccg tagcgtgcga gagcccgagc cgggccgcga tgttgatcga
     2941 gccgtaaccg ccggccagga gctcgaggat ctgctgctcg cggggcgaga gcgtgatgcg
     3001 ctcctccgga ggcccgaaga cgggctcggc cggcggcgcg acgacggacg tgacgcgcgc
     3061 gagcaatgcg tcgagcacgg cgccgtcgcc gttctcgaag acgtggacga gggtgcgata
     3121 ggcggcgccg ggcgaaggaa gcgcgatcgt gcgcgtcacg agccgcgcct tggtcccctc
     3181 ccgcgtccgc acgtccatca cgaacggcgc cgcggcctcg ccgcgcgagg acgccacggc
     3241 gacgccgcag tcccggaagc agcagatgtt cccgaacacg tcccgccccg cgaagacgtc
     3301 gtaacacttc ttgcccagga cctcgcctga ggtccagccg aggagcgccg tcgcggattc
     3361 gttccagtag acgatgcgat gcctcgggtc catgccgcac gccgccgcct gcgcgtcggc
     3421 gatgaggtcg agggcgtcgt cgatgcggag ggcgctgctt ctcatggcga ctgagacgca
     3481 atttggcggc gggccgctcc gctgtcaaga cgattcggtc tccgtcgtaa gcttgatttc
     3541 gccggagtgg cggaatggca gacgcagggg acttaaaatc cccggactcg caagggtcgt
     3601 gagggttcga tcccctcctc cggtaccagg ccccgggcgt cgggggtcgg ggcgggcgaa
     3661 cgcacgcgga cggacctgtc cccgccgcga taagatgatg aatctcatcc tcatgtctca
     3721 gcccggtgag atcaccgccc ttctgcgcga actcagatcc ggcgaccgga ccgccgggga
     3781 tcgcctgatc gaaaaggcct acccggagct tcatcgcatc gcgcaggccc actttcgaca
     3841 cgagcggccc ggccatctgt tgcagcccac ggccctcgtg aacgaggcgt ggcagaagtt
     3901 cgtggcgctc gacgcggtcg atctccggga ccgcgcgcat ttcctgtcgc tgtgctcgcg
     3961 gttgatgcgc cagatcctcg tcgatcacgc ccggcgccgc gcgacacacg agcgcgccgt
     4021 cgtttcgatg agcatcgacg accggattga agagaacatg ctcgatctgg atctcgcgct
     4081 gagcaagctc gaaaaggctc acccgcgtgc ggggcgcgtc atcgagctgc gctacttcgg
     4141 cggcatgtcg aacgaagagg tcgcgcaatg cctcgaggtt tcgcccgcca cggtcaagcg
     4201 cgactggctc gccgcccggg cgtggctgta cgggcagctc cgagggaatt ccgcgtgatc
     4261 gggcggcgct tcgaggacct ctccgggcgc ctggcggaag cgctggaatc ggccgacccg
     4321 gaggcccacg tcgccgcctc gggcgatccc gaactcgtcg aactcttcgc atggcatcgc
     4381 cgggcgggcg actttctgga aggcggcggc gcgaagcggg agaacccgtg ggtgggccgc
     4441 cacatcgccg gccggtactt gctgagatcc gtgctggggc ggggaggctc gggcgtggtc
     4501 ttcgaggcct gcgacgaaca ggtcgccggg cgccgcgtcg tcgtcaagct tctgcacgat
     4561 ttctggtcct cggaagactg gatgcgcagg cgctttcgcg aggaagccgg cgtgctggcg
     4621 cgactggatc atcccggcat cgtgagcctg atcgacgccg gcgagatcga ggacgggcgc
     4681 ctctttctgg ccctcccgtt tcacgaggga cgtacgttgc gcgaggcgct cgccgccggg
     4741 cctctcgacg ctccgttcgc cgcgcgcctc ctgcgcgaga tcggtgaggc cgtcggctac
     4801 gcacatgcag agggagtgct ccatcgcgac ctgaagcccg agaacattct gctggtgagg
     4861 cgcgcggacg gcgagcatcc gctcctgatc gacttcggca tcgcccaggt cggagatgcg
     4921 ggcgggccgc ccgagaccac gacccacctc atgggcagcg ccgtctacat ggctccggag
     4981 cacctgatgg gcaaggccga ggccgcgagc gacgtctatg cgctgggcgt catcgcatgg
     5041 gagatgctgg ccggagcgcg cccgttcgat tccgcctctc cgtttgccct gccggagctt
     5101 cagaggaagg gcgtgggcga tacgttctac cggctgcgcc cggacctcgg gaccggagtc
     5161 ggcaagctgc tggcgcgcgc tctggcgtac gaggcggtgc gccgtccggc gcccgtcacc
     5221 gccttcaccg gggagatcgc cgacgcgctc caggcgggcg cgatcgactc ctggctcgcc
     5281 cggctctggg tcgtgcgccg ctcgcggcgc tggatgctcg ccggcggcgc gacggcggtc
     5341 atgggggccg ccgccggagg atggtggctg cgggatcggc tgaccccgct cgacgcggag
     5401 gagcgcgtca ttgagtttcc tcggggtgcg acgcccgaca tggccggttt cagcattcgt
     5461 cgtgagctga cggagcgtgc catccgcgat ttcccgagcg gcgccgtcac ggcgatgcgc
     5521 tatctcaccg ccgatcaggg aacgctctac aggcgcctga gcgcgcggca gaaggagtgg
     5581 gcgtttcgcc ggggctggaa gctgtcggcg ctctgtcggc cggagtcggg acaggccggc
     5641 atcgccgtcg agaacggttc cgtcgccccc gccttcgtcg ccgtcctcaa ctcgacccga
     5701 gaccgcgtcg agctgatcgc cgtcacgcag atccgcaccg ggatcgctgg acttcgcgcc
     5761 cctctcagcc tcccgccggc gccgcgcctc gtgcaactgg agatggctta cgaccccgct
     5821 tccgcccggg ccaccgtctc cgtggacggc caggtcctcg ttcgcgacta cgccggccac
     5881 acggagtatc gaagcgatcc ctccgtcttt ttcgccctct cccgcacgga cgagacgatg
     5941 gcgtccgccg tcttcggcgg gcttcgcttc gagatcctcg ggtagcgaag cgcttccgaa
     6001 agcgctccga tcggaatgag ccgttttcac gcgctcgttc gcctacaggc aagagttgct
     6061 ctcgaggggg gcgtccatgc gactggccgc gggcttgctc gtcacgttcg gaattctggt
     6121 cactggggct gcgtctgctg cgggaacgga gcgtgacgcc acggaccaca cggcgttggt
     6181 cgctcagtgg caggcgccgc cctactggct tcctcccgcg cgcggcgccg aggcgtctgc
     6241 ctctggccgc cagcctctgg ccggcggacc cacggccttg ccgttcatcg cgctcttccc
     6301 ctgccgcctc gtcgacacgc gcgcgccggc cttcccggcg cccctcggcg ggggcttcct
     6361 gccgcccgcc acggtgcgaa gttacacgct gacgggcgtc tgcaacgtcc ccccgggagc
     6421 gcaggcgatc tcgctgaacg tgacggtcgt gaacccgacc gggccgggtt tcatcacgct
     6481 ataccctgag ggcggagcgt tcccgcccgt ctcgaccctc aactacctcg ggcacgacgt
     6541 gatcgtgaac gcggccgtcg taccgctcag cgcgacgggc gggatctcga tggcgctggg
     6601 ggtgtcgggc ggggacgtca ttctcgacac caacgggtac tacgcgaccg ttccgagcgt
     6661 gacgagcgtg aacacgctgg cgggagacct gaccctgtcg gcgggcagca acgtaacgat
     6721 cacgccctcg ggcaacacgc tcacgatcgc gtcgacgggc gggggtgggg gaacggtgac
     6781 gtcggtcggg accggctcgg ggctgacggg cggcccgatc acgacgacgg ggacggtctc
     6841 ggtggcccct ctcggcatca cgaccggcct cctcgcgaac aacgcggtca cgtcggcgaa
     6901 gctcgcgccg ggctctgtgg gcctcgccca gatcgacccg acgcaggtcc agggccgcgt
     6961 cgcgagcggg tgcccggacg ggctctcgat ccagtcgatc aatcaggacg ggtccgtggc
     7021 ctgcctcgca tcctctccac aggcgggctt cctgatctcc acgatcgaca gcgtgggcga
     7081 cgtcggccag tacacctcga tcacgaccgg ttgggacggc ctcggcctga tcagctatta
     7141 cgacgccacg aacggcgacc tcaaggtcgc ccactgctcg aacccgctgt gcacgaccgc
     7201 caccatcacg accctcgaca gcgcgggaga cgtcgggctg acctcctcga tcacgatcgg
     7261 cgccgacagc ctcggcctga tcagctatta cgacgccacg aacggcgacc tcaaggtcgc
     7321 ccactgctcg aacctgtcgt gcacgagcgc cacgatcacg accctcgaca gcgccggcga
     7381 cgtggggctc cactcctcga tcacggtcgc ggccgacgga gcggctctga tcagctactt
     7441 cgacgcgacg aacggcggcc tgaaggtggc gcactgcgtg aatttgagct gtacctcggc
     7501 catcaagtcc acgatcgaca ccggaggcgt cggaaaccac tcctcgatcg cgatcggggc
     7561 cgacgcgcga ggcatcatca gctattacga cgcgacgaac ggcgatctga aggtggccca
     7621 ctgctcgaac gccgcgtgta cgagcgccac cgtgacgacc ctcgacagcg cgggcgacgt
     7681 ggggctcttc acgtccatca ccgccggggg cgacgggagc ccggcgatca gctattacga
     7741 caacacgaac ggccgtctca aggccgcctc ctgctcggac tttctctgct cgacggccag
     7801 catcttcacg atcgacggct cgggcgacgt cggccgattc ggctcgtcgc tcacgatcgg
     7861 tcccgacggt ttcggcctcg tcagctacct cgatttgacg aacttcgttc tcaaggtggc
     7921 ccattgcggg agtgcgggat gcaccgcgac cgtcgtcgac aacagctctc ccgcgcacgt
     7981 cggccgatac tcgtcgctca cgatcggccc cgacggcttc gccctgatca gctactacga
     8041 cttcacgaac cagaacctca aggtggcgca ctgctcgaac aatctctgcc tgccctgggt
     8101 ccggcggagg tgaagaaggc gggaccgcct tagactccgc gcgtggagaa cgacgaggcg
     8161 gacgggctcg acgccgtgga gcgggcgctc ggccacgcgt ttcgcgaccg gcggctcctg
     8221 gtccgggcgc tcacgcactc gtcgtacgcg aacgacgatc ccgacgccga agacaacgag
     8281 acgctcgagt ttctgggcga cagcatcctg aactacctcg tctccgagat gctcttcgac
     8341 gcgttcccgc tcgaggccga ggggacgctc tcgaaggcgc ggtccgttct cgtctcggag
     8401 aaccacttcg cgtacctcgc gcgccggatg gacctgggct cggcgctgcg gctgtcgccc
     8461 ggcgaggaac gcgccggcgg aaggaaccgc gacgcccggc tcgcggacgc gttcgaggcc
     8521 gtcttcgcgg cgctcgcgct cgacgccggg ctggacgagg cgcgcgcgct ggcgcgaagg
     8581 ctcttcgcag aagacatcgc cgcgctcgac ctcgggggtc ttcatcgccg cgaccccaag
     8641 accgcgctcc aggagcgcgc gcagggcgag gggctcggcc tgccgctcta ccgtctcgtc
     8701 gaggaatccg gcccggctca cgagcgtcga ttcgtctacg aggtgactta cggcgaaggg
     8761 gagcgagcga cgggagccgg aagctcgaag aaggaagccc agacggccgc cgcggaggcg
     8821 atgctgagga agagggaaga ggggatttct tagaagggtg agggaggcct cggcggttcc
     8881 tgcggcccct gcgccacgaa gcggcgaacc gcccgattca ccttctaaga agatcttctc
     8941 tcacctcgtc aggtctgcct tcagcagcac ccagccctcg agcgccttct gcacccgggc
     9001 gtccgtgaac gtcttgtggt cgagctcctt gcagggcagg caccaatccg catagaagtc
     9061 gatcatcacg ggcttgccgg ccgccttcgc cgcggcgacc gccgcgtcgg aatacggcgc
     9121 gaacttcagc tcgggccccg ccttgcgcgg gagcgcgaac gcggcgccgg ccgcgaggag
     9181 gcccacggcg acggcgacct tcacggccgc gaagccgcgg acggaggcgc cggtcttctc
     9241 gaagaagagg aaccagatcc cgcccgcgag gagcggcagc gcgacgccga tctcgtagac
     9301 gcggggcgcg agcagcgggc gcaggaagta gacggcgagc gcgaggagcg cgaagccgaa
     9361 gaccttccgc acggccgtca tccactcgcc cgaccgcggg agcgccttga ggctgcccga
     9421 gaccgtgccg aggacgaggt acggcaggcc gagtcccatc gcgagcgtga agaagagcgc
     9481 gaagccgagc ggcgccgagc ccttcgccgc gacgtacgtg aggagcgaca ggacgaacgg
     9541 cccgatgcag ggggccgcga cgaagccgac gaagaggccc atcgtgagcg cgccgagggc
     9601 gccggtctgc gagccggtgc ggtccgtgac gaagtgcggg gcctggatct cgtagagccc
     9661 gaacatcgag agcgcgagag cgacgacgat cgccgcaatc ccgatgatca cggccggctt
     9721 ctgcagccac gcgccgaaga gcgagcccga gagggcggcg aagacgccga gcgccgagta
     9781 cgtgacgctc atcccgagga cgtaggcgac cgcgagcccg aacgtcccgc ccttcctgcc
     9841 gccgctctgc cggctgaaga agccgagcgt gatcgggatg agcgggtaga cgcacggcgt
     9901 gaggttcagc gcgagccccg agacgaagac gaggccgagg aggaggggga gggacttgcc
     9961 ggcgaggccg ccgctctcct ccgtggagcc gccggcgatc cggctgagga actgctcggg
    10021 cggctcgaac ccgacgacgc ggccgccgcg ctccttcccg tcggggccga ggaagagaat
    10081 cgtcgggacg ccgacgacct tccacgcctc gcgcagggcg gcggtgccgc cgggcgggcc
    10141 gccggacggc gcgctgccgt ccgccccggc gtccgcccat cagccgtcag gctgtcgagc
    10201 atctcggcgg
//
LOCUS       DAOMFY010000129        10131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2029_length_10131_cov_7.432811, whole genome shotgun sequence.
ACCESSION   DAOMFY010000129 DAOMFY010000000
VERSION     DAOMFY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2029_length_10131_cov_7.432811"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3971
                     /gene="rpoC"
                     /locus_tag="PLB01_11320"
     CDS             <1..3971
                     /gene="rpoC"
                     /locus_tag="PLB01_11320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQQ77935.1"
                     /translation="KRMKHRGVVCDKCGVEVTKSKVRRERMGHIELAAPVSHVWFFKG
                     LPSRIGHLLDLTLRDLERILYFEQYVVIDPGPLSEDENLEIKSLITEEKFRELKAKYG
                     VDAFVAKMGAEAIQELLQSVNIDDLAEELRLVMKTETSALKRLKAAKRLKVVDAFRRS
                     GHRPEWMILNVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKKLLELRAP
                     EVIVRNEKRMLQEAVDALFDNGRRGRVLKGSNNRPLKSLSDTLKGKSGRFRQNLLGKR
                     VDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYHRLEQKGITSTIKASKELVEQA
                     PNEVWDALEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIEGKAIKIHPLVCAAFNADFD
                     GDQMAVHVPLSPKAQVEAQVLMLSSHNILSPAHGKPLTVPSQDLVLGLYYLTRRKEGC
                     KGEGRVFSSFDEVVLAHDAGEVDTHAGIAVRFTGSLIDLESQVLNDQDIVHTDPIACN
                     RTRIETTVGRVLLSANLPPELPFLNGTLKKKGLQDLVNYCFVTYGLERTVEILDIVKA
                     LGFTVATRAGISIGVDDMRIPGNKTELVSRARKEVLEVENQRQQGAITDGERHNKIID
                     IWHRITETVAEAMFKEMKASDGVAGGEFNPIYMMADSGARGSKEQVRQLAGMRGPMSK
                     PSGEVMEQPIISNFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRRLVDVAQDV
                     IITQEDCETVDGIVVSAIVEGGEILEPLRDRIVGRIVQEDVFDPASDEALVAANDTIT
                     EELAGKIQEAGIERVKIRSVLTCETRRGVCRKCYGRNLATGDLVDIGEAVGVIAAQSI
                     GEPGTQLTMRTFHYGGTARVSEAAKHVAKNPGIIKLLNANTVSKKDGTKVVVNRNSKL
                     VLLDAKGREKERYSLAYGSVLKVVEGEEVKPGQLLVSWDPFASPILSEIAGKVAYKDI
                     LEGENLREETDKVTGLTQKIVVETTGTTEKRSPTLVVTGKNGEERKYALPIPSHLMIA
                     DGDVVNPGDALAKKPVEKKRTRDIVGGLPRVVELFEARRPKEPAVITEISGVVRHAGT
                     VKGQQKIVVESEDGQKREMLVPRGAHVMVQDGERVEAGDPLTEGDPSPHDILAVKGEK
                     ELQRYMTDKIQEVYRSQGVGINDKHIEVIVRQMMRSVRIEEVGDTEFLVDEQVDRFRF
                     NEENERILNAGGKAAQGRPLLLGITKASLSTDSFVSAASFQETTRVLTEASIAGRVDL
                     LRGLKENVIVGRLIPAGTGMDYYRNVVLEREEIPPAPEETEELYFDDETPVIAADLDD
                     EV"
     gene            complement(4026..5771)
                     /locus_tag="PLB01_11325"
     CDS             complement(4026..5771)
                     /locus_tag="PLB01_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77936.1"
                     /translation="MKEVALPISVLLALGLSSAAAAAPPTSAAPLRFEISFSKDVSPG
                     PLDGRVLLIVSKDGSEEPRKQVGIGLKTQQIFGVDVDRLAPGSPAVVDASVLGYPLES
                     LAQVPKGTYVVQAVLHRYETFHRADGHVVKLPMDRGEGQQWNLAPGNLVSTPRKVEVD
                     PAKGGTVAVALDGMIPPIPEPKTTKFVRHERIRSERLSTFWGRDMFLGAHVLLPLGFD
                     DHPNARYPFVIFHGHFPKTIEGFREEPPDPDLKPEYSERFRLEGYNRIQQEQAWQLYK
                     DWTSPGFPRAIVVEIQHANPYYDDSYAVNSANLGPYGDAIEYELLPYLEKKYRAIGAG
                     WARFAYGGSTGGWEAMAVQIFYPDDYNGIYAACPDPIDFRANTVFDLYAEANAYYPDA
                     PWKRIPRPGHRDSFGRIAATVEDTNRYELVLGTKGRSGEQWDIWQAVYSPVGADGYPK
                     PIWDKRTGVIDKEVAEYWKEHYDLTAILKRDWDKGLGKKLEGKLHIFVGEADNYFLNN
                     AVVLAEEVLKSVKNPPSGAFVDYEPRAEHCWNGDHTRPNAISRLRYHQFFLPKIVERI
                     RKTAPPGADLKSWDY"
     gene            5803..6729
                     /locus_tag="PLB01_11330"
     CDS             5803..6729
                     /locus_tag="PLB01_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HQQ77937.1"
                     /translation="MADLLDLLEKTSRTFALSIPPLPEPVRREVTVAYLLFRIADTFE
                     DATHWPPAQRIAALEDFGFLLKEGTRAEAERLGQAWAQAGASRHAGYRELIAETPFVF
                     EAFAALRPEAIEILRDHVVRSSEGMARFVARTDEHGLTLDSLEDLKNYCYTVAGIVGE
                     LLTELFLLEEISLRAAAPDLRMRARTFGEALQLVNILKDSAEDAAEGRRYLPSGVPRA
                     DVFALARRDLGVAGEYIETLHCASAPPGILEFTALPVALAWAALAKVEERGPGAKVGR
                     PEVFRIARGVRGSIARGERPALYDRTLSGEPR"
     gene            6726..8669
                     /gene="dxs"
                     /locus_tag="PLB01_11335"
     CDS             6726..8669
                     /gene="dxs"
                     /locus_tag="PLB01_11335"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQQ77938.1"
                     /translation="MTLLDTINGPDDLRGLDHEALARLAAECRAVIVETITRSGGHLA
                     SNLGVVELTLAMHRAFHSPKDRFVWDTSNQCYTHKLVTGRAGGFATIRKPGGLSGFAE
                     PMESDHDTFAAGHAGTGLSFGMGLAVAHQGDADDPYVVVVVGDGAMTSGTSYEALNNI
                     VHIKPKRLVVVLNDNGWSISENVGWLAHWRNRFELHPAYKRLTEKGHGLFKKLPKGEE
                     AWGLAKKIKNSVEGLFFPNLVWDELGFHYVGPIDGHDFKELEEALARAKDVSRDGTPV
                     VIHALTHKGRGYDKAEENPSRFHQPGTPSPAASSGAAPTYSQVFAKTLLTMMEKDPKI
                     VAITAAMLEGTGLGEVQKVYPGRVFDVGIAEEHAVIMAAGMAKAGLKPVVSIYSTFLQ
                     RGYDQLIHDVCLQNLPVTVCIDRAGLVGDDGKTHQGIYDIAYTRGIPNMTVAAPKDEN
                     ELQHLLFTAIGSGKPFAVRYPRGAGLGVPLDSGLLAIPAGKGEILSVGKDLNLLAYGS
                     MVSVAEKAAGVLRARGVDCGVANARFVKPLDMELVGALLAASPRLMTLEEHLQQGGFG
                     SAVLEAVHAAGLPSQGVRSHAIPDQFVEHGPQAFQRQHFRLDPEGVLARIVELYPGLL
                     AADGSAPAAPAGSRVASEPVHWT"
     gene            8872..9246
                     /gene="rpsL"
                     /locus_tag="PLB01_11340"
     CDS             8872..9246
                     /gene="rpsL"
                     /locus_tag="PLB01_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQQ77939.1"
                     /translation="MPTISQLVRKGRDAVRYKTKSPALQSCPQRRGVCTQVKTTTPKK
                     PNSALRKIARVRLTNGIEVTTYIPGIGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGTL
                     DAVGVANRKQSRSKYGAKRPKA"
     gene            9256..9726
                     /gene="rpsG"
                     /locus_tag="PLB01_11345"
     CDS             9256..9726
                     /gene="rpsG"
                     /locus_tag="PLB01_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQQ77940.1"
                     /translation="MPRRREVPKREVLPDPLYQSQLVTKFINCVMERGKKSTAESIFY
                     GAMKQIEQKTSDDPLKTFKKALENVKPVLEVKSRRVGGSTYQVPVEVKPNRRTSLAIR
                     WVISYAQARGEKTMRDKLAGELLDAANNRGNAVKKKEDTHKMAEANKAFAHYRW"
     gene            9923..>10131
                     /locus_tag="PLB01_11350"
     CDS             9923..>10131
                     /locus_tag="PLB01_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQQ77941.1"
                     /translation="MPRNHPLEMCRNIGIMAHIDAGKTTTTERVLYYTGVNYKIGEVH
                     DGTATMDWMVQEQERGITITSAATT"
BASE COUNT         1800 a         3447 c         3364 g         1520 t
ORIGIN      
        1 acaagcggat gaagcaccgc ggcgtcgtct gcgacaagtg cggcgtcgag gtcacgaagt
       61 cgaaggtccg gcgcgagcgc atgggccaca tcgagctcgc ggcccccgtg tcccacgtgt
      121 ggttcttcaa ggggctgccg agccgcatcg ggcacctcct cgacctcacg ctccgcgacc
      181 tcgagcggat cctgtacttc gagcagtacg tcgtcatcga cccgggcccg ctctccgagg
      241 acgagaacct cgagatcaag agcctcatca cggaagagaa gttccgcgag ctcaaggcga
      301 agtacggcgt cgacgcgttc gtcgcgaaga tgggcgccga ggcgatccag gagctcctcc
      361 agagcgtcaa catcgacgac ctggccgagg agctgcgcct cgtcatgaag acggagacgt
      421 ccgccctcaa gcgcctcaag gccgccaagc gcctgaaggt cgtcgacgcg ttccgccgct
      481 cggggcaccg cccggagtgg atgatcctga acgtcatccc ggtcatcccg ccggagctcc
      541 gtcccctcgt gcccctcgac ggcgggcgct tcgcgacgtc ggacctgaac gacctctacc
      601 gccgcgtcat caaccgcaac aaccgcctca agaagctcct cgagctccgt gcgcccgaag
      661 tcatcgtgcg caacgagaag cgcatgctcc aggaggccgt cgacgccctg ttcgacaacg
      721 gccgccgcgg ccgcgtcctc aagggatcga acaaccgccc gctcaagtcg ctttccgaca
      781 cgctcaaggg caagtcgggc cgcttccggc agaacctcct cggcaagcgc gtcgactact
      841 cgggccgttc ggtcatcgtc gtggggccgg agctcaagct ccaccagtgc ggcctgccga
      901 agaagatggc cctcgagctg ttcaagccgt tcatctacca ccggctcgag cagaagggga
      961 tcacgtccac gatcaaggcg tccaaggagc tcgtcgagca ggcgccgaac gaggtgtggg
     1021 acgcgctcga ggaggtcatc aaggagcacc cggttctcct gaaccgcgcg cccacgctcc
     1081 accgcctcgg catccaggcg ttcgagccgg tcctcatcga gggcaaggcg atcaagatcc
     1141 acccgctcgt ctgcgcggcg ttcaatgcgg acttcgacgg cgaccagatg gccgtccacg
     1201 tcccgctgtc gccgaaggcg caggtcgagg cgcaggtcct gatgctgtcc tcgcacaaca
     1261 tcctgtcgcc cgcgcacggc aagcccctca cggtgccgtc ccaggacctc gtcctcggcc
     1321 tctactacct cacccgccgc aaggaagggt gcaagggcga gggccgcgtc ttctcgagct
     1381 tcgacgaggt cgtgctcgcg cacgacgccg gcgaggtcga cacgcacgcc gggatcgcgg
     1441 tgcgcttcac gggcagcctc atcgacctcg agagccaggt cctcaacgac caggacatcg
     1501 tccacacgga cccgatcgcc tgcaaccgca cgcgcatcga gacgacggtc ggccgcgtcc
     1561 tgctctcggc gaacctgccg cccgagctcc cgttcctgaa cggcacgctc aagaagaagg
     1621 gcctgcagga cctcgtcaac tactgcttcg tgacctacgg gctcgagcgc acggtcgaga
     1681 tcctcgacat cgtgaaggcc ctcggcttca ccgtcgcgac ccgcgccggc atctcgatcg
     1741 gcgtggacga catgcggatt ccgggcaaca agaccgagct cgtgagccgc gcccgcaagg
     1801 aagtgctcga ggtcgagaac cagcgccagc agggcgccat cacggacggc gagcgccaca
     1861 acaagatcat cgacatctgg caccggatca ccgagaccgt ggcggaagcc atgttcaagg
     1921 agatgaaggc gtcggacggc gtcgcgggcg gggagttcaa ccccatctac atgatggccg
     1981 actccggcgc ccgaggctcg aaggagcagg tccggcagct cgcgggcatg cgcggcccca
     2041 tgtccaagcc ctcgggcgag gtcatggagc agccgatcat ctcgaacttc cgcgaaggcc
     2101 tcaccgtgct cgagtacttc atctcgacgc acggcgcgcg caagggcctc gcggacacgg
     2161 cgctcaagac ggccgactcc ggctacctca cgcgccgtct cgtcgacgtc gcgcaggacg
     2221 tcatcatcac gcaggaagac tgcgagaccg tcgacgggat cgtcgtgtcg gcgatcgtcg
     2281 agggcggcga gatcctcgag ccgctccgcg accgcatcgt cggccgcatc gtccaggaag
     2341 acgtcttcga cccggcgagc gacgaagccc tcgtcgccgc gaacgacacg atcaccgagg
     2401 agctcgccgg caagatccag gaggccggca tcgagcgggt caagatccgc tcggtcctca
     2461 cgtgcgagac gcgccgcggc gtctgccgga agtgctacgg gcgcaacctc gcgacgggcg
     2521 acctcgtcga catcggcgag gcggtcggcg tcatcgccgc ccagtccatc ggcgagcccg
     2581 gcacgcagct cacgatgcgg acgttccact acggcggcac ggcccgggtc tcggaggcgg
     2641 cgaagcacgt cgcgaagaac cccggcatca tcaagctgct caacgcgaac acggtcagca
     2701 agaaggacgg cacgaaggtc gtcgtcaacc gcaactcgaa gctcgtcctc ctcgacgcca
     2761 aagggcgcga gaaggagcgc tactcgctcg cttacggctc cgtcctcaaa gtggtcgagg
     2821 gcgaagaggt gaagccgggc cagctcctcg tgagctggga cccgttcgcg tcgccgatcc
     2881 tctcggagat cgcgggcaag gtcgcctaca aggacatcct cgaaggcgag aacctgcgcg
     2941 aggagacgga caaggtcacg ggcctcacgc agaagatcgt cgtcgagacg accggcacga
     3001 ccgagaagcg ctcgccgacg ctcgtcgtca cgggcaagaa cggggaagag cgcaagtacg
     3061 cgctgccgat cccgtcgcac ctcatgatcg cggacggcga cgtcgtgaac cccggcgacg
     3121 ccctcgcgaa gaagcccgtc gagaagaagc gcacccgcga catcgtcggc ggtctcccgc
     3181 gcgtcgtcga gctgttcgag gcgcggcgcc cgaaggagcc ggccgtcatc acggagatct
     3241 ccggcgtcgt gcgccacgcg ggcacggtca agggccagca gaagatcgtc gtcgagtccg
     3301 aggacggcca gaagcgcgag atgctcgtcc cgcgcggcgc gcacgtcatg gtccaggacg
     3361 gcgagagggt ggaagcgggc gatccgctca ccgagggcga tccgtcgccg cacgacatcc
     3421 tcgcggtcaa gggcgagaag gaactgcagc ggtacatgac cgacaagatc caggaggtct
     3481 accgttcgca gggcgtcggc atcaacgaca agcacatcga ggtcatcgtc cgccagatga
     3541 tgcggagcgt ccgcatcgag gaggtcggcg acacggagtt cctcgtcgac gagcaggtcg
     3601 accgcttccg gttcaacgag gagaacgagc gcatcctgaa cgccggcggc aaggccgccc
     3661 agggccgccc gctgctcctc gggatcacga aggcatcgct ctcgacggac tcgttcgtct
     3721 cggccgcgtc gttccaggaa acgacgcgcg tcctgacgga ggcgtccatc gcgggccggg
     3781 tggacctcct ccgcggcctc aaggagaacg tcatcgtcgg gcggctcatc cccgccggca
     3841 ccggcatgga ctactaccgc aacgtcgtcc tcgagcgcga ggagatcccg cccgcgcccg
     3901 aggagaccga ggagctctac ttcgacgacg agacgccggt catcgcggcg gatctcgacg
     3961 acgaagtctg acccctcgat tccatcgttc cttcaagggc cggcgcaagc cggccctttt
     4021 cttttttagt agtcccagct cttcaggtcc gccccgggcg gagccgtctt ccggatccgc
     4081 tcgacgatct tcggcaggaa gaactggtgg tacctcaggc gcgagatcgc gttcgggcgc
     4141 gtgtggtcgc cgttccagca gtgctctgcg cgcggctcgt agtcgacgaa cgccccggac
     4201 ggcgggttct tcacggactt caggacctct tccgcgagga cgacggcgtt gttcaggaag
     4261 tagttgtccg cctcgccgac gaagatgtgg agcttgccct cgagcttctt cccgagcccc
     4321 ttgtcccagt cgcgcttcag gatcgccgtg aggtcgtagt gctccttcca gtactcggcg
     4381 acctccttgt cgatcacgcc cgtccgcttg tcccagatcg gcttcgggta gccgtcggcc
     4441 cccacgggcg agtagacggc ctgccagatg tcccactgtt cgcccgaacg gcccttcgtc
     4501 ccgaggacga gctcgtaccg gttcgtgtcc tcgacggtcg ccgcgatacg gccgaaggag
     4561 tcccggtggc cggggcgcgg gatgcgcttc cacggcgcgt cgggatagta ggcgttcgcc
     4621 tccgcgtaga ggtcgaagac cgtgttcgcg cggaagtcga tcgggtcggg gcaggccgcg
     4681 tagatcccgt tgtagtcgtc gggatagaag atctggacgg ccatcgcctc ccagccgccc
     4741 gtcgacccgc cgtacgcgaa ccgcgcccag ccggcgccga tcgcgcggta cttcttctcg
     4801 aggtacggga ggagctcgta ctcgatcgca tcgccatacg gaccgagatt cgccgagttc
     4861 acggcgtacg aatcgtcgta gtacgggttc gcgtgctgga tctcgacgac gatcgcgcgc
     4921 gggaagccgg gcgacgtcca gtccttgtag agctgccagg cctgctcctg ctggatccgg
     4981 ttgtagccct cgaggcggaa gcgctcgctg tattccggct tgaggtccgg gtcgggcggc
     5041 tcctcgcgga agccctcgat cgtcttcggg aaatgcccgt ggaagatgac gaacggataa
     5101 cgggcgttcg ggtggtcgtc gaagccgaga ggcaggagga cgtgggctcc gaggaacatg
     5161 tcccgacccc agaacgtcga caggcgctcg ctccggatgc gctcgtggcg tacgaatttc
     5221 gtcgtcttcg gctcgggaat cggcgggatc atgccgtcga gggcgaccgc gacggtgccc
     5281 cccttcgcgg ggtcgacctc gaccttccgg ggcgtcgaga ccaggttccc gggggcgagg
     5341 ttccactgct ggccctctcc gcggtccatc gggagcttca cgacgtgccc gtccgcgcgg
     5401 tggaacgtct cgtagcggtg gaggacggcc tgcacgacgt acgtcccctt cgggacctgg
     5461 gcgaggctct cgagcgggta tccgaggacg ctcgcgtcga cgacggcggg ggagcccggc
     5521 gcgagccggt ccacgtcgac gccgaagatc tgctgcgtct tcaggccgat cccgacctgc
     5581 ttgcgcggct cctctgagcc gtccttcgag acgatcagga ggacgcgccc gtcgagggga
     5641 cccggggaga cgtccttcga aaaggagatt tcgaatcgca gcggggcggc ggatgtcggc
     5701 ggtgcggccg ccgccgccga agagagaccc agagcgagca gcacggaaat cgggagcgcg
     5761 acctctttca tgggggcgag agtaccccgg taacatgggc gcgtggcgga cctcctcgat
     5821 ctcctcgaga agacgagtcg cacgttcgcg ctctcgatcc cgccgctgcc cgagcccgtg
     5881 cggcgcgagg tgacggtcgc ctacctcctc ttccggatcg cggacacgtt cgaggacgcg
     5941 acgcactggc cgccggcgca gcgaattgcg gcgctggagg atttcggatt tctgctgaag
     6001 gaaggaacgc gggcggaggc ggagcggctc gggcaggcct gggcgcaggc cggcgcttcg
     6061 cgtcacgcgg gttaccgtga actgatcgcc gagacgccgt tcgtgttcga ggccttcgcc
     6121 gctcttcggc ccgaagcgat cgagatcctt cgtgaccacg tggtccgttc gtcggagggg
     6181 atggcgcgtt tcgtcgcgag gacggacgaa cacgggctga cgctcgactc cctcgaggat
     6241 ctcaagaact actgctacac cgtggccggg atcgtgggcg agctcctgac ggagctcttt
     6301 ctgctcgagg agatctctct tcgagcggcg gcaccggatc tccgcatgcg agccaggacg
     6361 ttcggcgagg ccctccagct cgtcaacatc ctcaaggact ccgccgagga cgcggccgag
     6421 ggccgccggt acctgccctc gggcgtcccg cgcgccgacg tcttcgcgct cgcccggcgc
     6481 gacctcggcg tggcggggga atacatcgag acccttcact gcgcgagcgc gccgccgggg
     6541 attctcgagt tcacggcgct tcccgtggcg ctcgcctggg ccgcgctcgc gaaggtcgag
     6601 gagcggggcc cgggagcgaa ggtcgggcgg cccgaggtct tccgcatcgc gcgcggcgtg
     6661 cgcggatcga tcgcgcgtgg tgagcgcccg gccctgtatg atcgaacgct ttcgggggag
     6721 cctcgatgac ccttctcgac accatcaacg ggccggacga ccttcggggc ctcgaccacg
     6781 aagcgctcgc ccgcctcgcg gccgaatgcc gcgcggtgat cgtcgagacg atcacgagat
     6841 cgggcggcca cctcgcctcg aacctcggcg tcgtcgagct cacgctcgcg atgcaccgcg
     6901 cgttccacag cccgaaggac cgcttcgtgt gggacacgtc gaaccagtgc tacacgcaca
     6961 agctcgtcac ggggcgtgcg ggcggcttcg cgacgatccg caagccgggc ggcctctcgg
     7021 gcttcgccga gccgatggag agcgaccacg acacgttcgc cgccggacac gcggggaccg
     7081 gtctctcgtt cgggatgggc ctcgccgtcg cccaccaggg ggacgcggac gatccgtacg
     7141 tcgtcgtcgt ggtcggcgac ggcgcgatga cctcgggcac gagctacgaa gcgctcaaca
     7201 acatcgtgca catcaagccg aagcggctcg tcgtcgtcct gaacgacaac ggctggtcca
     7261 tctcggaaaa cgtcggctgg ctggcccact ggcgcaaccg cttcgagctg cacccggcgt
     7321 acaagcgcct cacggaaaag gggcacgggc tcttcaagaa gctcccgaag ggcgaggagg
     7381 cctgggggct cgccaagaag atcaagaact ccgtcgaggg gctcttcttc ccgaacctcg
     7441 tgtgggacga gctcggattc cactacgtcg ggccgatcga cggccacgac ttcaaggagc
     7501 tcgaggaggc cctcgcgcgc gcgaaagacg tctccaggga cggaacgccc gtcgtgatcc
     7561 acgcgctcac gcacaagggt cgcggctacg acaaggcgga ggagaacccg tcgcgtttcc
     7621 accagccggg aacgccctcg ccggccgcgt cctcgggggc ggcgcccacg tactcgcagg
     7681 tgttcgcgaa gacgctcctc accatgatgg agaaggaccc gaaaatcgtc gcgatcaccg
     7741 ccgcgatgct cgaggggacc gggctcggcg aggtccagaa ggtctacccg ggccgggttt
     7801 tcgacgtggg aatcgccgag gagcacgccg tcatcatggc ggccgggatg gccaaagcag
     7861 ggctgaagcc ggtcgtctcg atctactcga cgttcctgca gcgcggctac gaccagctca
     7921 tccacgacgt ctgcctgcag aacctgcccg tgacggtctg catcgaccgg gccggcctcg
     7981 tcggggacga cggcaagacg caccagggca tctacgacat cgcgtacacg cggggcatcc
     8041 cgaacatgac ggtcgcggcc ccgaaggacg agaacgagct gcagcacctt ctcttcacgg
     8101 cgatcgggtc cggcaagccc ttcgcggtcc ggtacccgcg cggggccggc ctcggcgttc
     8161 cgctcgactc cggcctgctg gcgattccgg ccggaaaagg cgagatcctt tcggtcggaa
     8221 aggacctgaa ccttctcgcc tacgggtcga tggtgtcggt ggcagagaag gccgcaggcg
     8281 tcctgagggc tcgcggcgtc gactgtggcg tggctaacgc cagattcgtc aagcctttgg
     8341 acatggagct cgtcggggcg cttctggccg cgagcccccg gctcatgacc ctggaagagc
     8401 accttcagca gggagggttc ggaagcgccg ttctggaagc cgtgcacgcc gccgggctgc
     8461 cgtcgcaggg cgtccgttcg catgcgatcc cggaccagtt cgtcgagcac ggcccccagg
     8521 cgttccagcg ccagcacttc cggctggacc cggaaggcgt cttggcccgg atcgtggagc
     8581 tttatccggg cctcctggcg gcggacgggt cggctccagc ggcgccggct ggctccaggg
     8641 tcgcctccga gcccgttcac tggacctgaa actctctcca cggaggggtt tgtcgcttga
     8701 cagcccttcg gggggtcact atgattcgcg ggtttccccg gggaccggcc cggcgttatc
     8761 cgccgtgtcg cgcccgggaa acggcctccc tgcggccggt tcgccactcg atagaccgtc
     8821 tttcaccgga atccgactcg ccaagcgttc cgaaggagtc tcgaaagtcc catgccgacg
     8881 atcagccagc tcgtgagaaa gggccgcgac gccgtccgtt acaagacgaa gtcgcccgcc
     8941 cttcagtcct gtccgcagcg ccgcggcgtg tgcacccagg tgaagacgac gaccccgaag
     9001 aagccgaact cggctctccg gaagatcgcg cgcgtgcgcc tcacgaacgg catcgaggtg
     9061 acgacgtaca tcccgggcat cgggcacaac ctccaggagc actccatcgt catgatccgc
     9121 ggaggccgcg tgaaggacct cccgggcgtc cgctaccaca tcatccgcgg gacgctcgac
     9181 gccgtcggcg tcgcgaaccg caagcagtcg cgctccaagt acggcgccaa gcgcccgaag
     9241 gcttaaggaa agagaatgcc gcgtcgccgt gaagtcccga agcgcgaggt cctcccggac
     9301 ccgctctacc agtcccagct cgtgacgaag ttcatcaact gcgtcatgga gcgcggcaag
     9361 aagtccaccg ccgagtcgat cttctacggc gcgatgaagc agatcgagca gaagacgtcc
     9421 gacgacccgc tcaagacatt caagaaggct ctcgagaacg tcaagccggt tctcgaggtc
     9481 aagtcgcgcc gcgtcggcgg ctcgacgtac caggttcccg tcgaggtcaa gcccaaccgg
     9541 cggacctcgc tcgcgatccg ctgggtcatc tcgtacgcgc aggcgcgcgg cgagaagacg
     9601 atgcgggaca agctcgcggg agagcttctc gacgccgcca acaaccgggg caacgccgtg
     9661 aagaagaagg aggacaccca caagatggcc gaggccaaca aggccttcgc ccattaccgc
     9721 tggtagaact gccacatccc gggtgaaggg aaaccgtccg gcctcagaac cggaaaggtc
     9781 tcttcgaaaa cccggacacg cccaaagcgt gcggaattca cgaggataga gccgggcgcc
     9841 tttgcttcgg cccagttcgc gaagcgaatt gcagaacctc tggattcgac ggcgcagtca
     9901 gcccggaacg ggacacaagc tcatgccgag aaatcatccc ctcgagatgt gccgaaacat
     9961 cggcatcatg gcccacatcg acgcgggcaa gacgacgacg accgagcgcg tcctctatta
    10021 caccggcgtc aactacaaga tcggcgaggt ccacgacggg accgcgacga tggactggat
    10081 ggtccaggag caggagcgcg gcatcacgat cacgtcggcc gcgacgacct g
//
LOCUS       DAOMFY010000130        10120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2032_length_10120_cov_6.819672, whole genome shotgun sequence.
ACCESSION   DAOMFY010000130 DAOMFY010000000
VERSION     DAOMFY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2032_length_10120_cov_6.819672"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLB01_11355"
     CDS             complement(<1..611)
                     /locus_tag="PLB01_11355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ77942.1"
                     /translation="MTLPDSAVARLREAVRAPDLSGTRYRLAGTIGAGGMGAVFEVED
                     EILGRRLALKVVDVSLEGEGPAEALLAEARVLARLEHPGLVPVHDAGRLPDGRAFYAM
                     KLVRGEGLAAAAARAVTLPERLRLFLRVCEPVAFANAAGVVHRDLKPSNVMVGPFGEV
                     LVLDWGLARAAASGPADASGTPGFMAPEQASGGPVDARADVYGL"
     gene            complement(608..1336)
                     /locus_tag="PLB01_11360"
     CDS             complement(608..1336)
                     /locus_tag="PLB01_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ77943.1"
                     /translation="MAPFPDTRGSLVAAVTGGDAAARSRALGTLAEAYWKPIYTYVRL
                     RAGRSHEEAADLTQEFFASLVEKDGLSRFDPSKARLRTYLRVLVDGLAANEWKARSRL
                     KRGGGSEVLSLDLEAVRREVDARRGPGDLSPEDLFEREWARSVFELAVARLRERCSAS
                     GHESRFALFSAYDLEDGEAGERPRYEDLALRFGTTAVDVTNQLAAARREFRGIVLDVL
                     RELTVSDAEFRAEARALLGVEPPS"
     gene            1448..2320
                     /locus_tag="PLB01_11365"
     CDS             1448..2320
                     /locus_tag="PLB01_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQQ77944.1"
                     /translation="MRWSNDGGGLGPDIEDRRGEGGGGFNFGGGGLRLGCGGIILLGV
                     LSLIFKQNFFALLSPQGGAPQARVDSRPVAPPSPKEEERARFVNFVVNDVQKTWDQKF
                     PGLGRNYTHAKLVLYRDATRSGCGFAQAQMGPFYCPADRKVYLDMGFFDELSQRFGAP
                     GDFAQAYVIAHEFGHHVQTLLGIEGGVRRAMAQNPSQEKALSVRLELQADCLAGVWGH
                     GTAERNILENGDVEKGLAAAAAVGDDRLQRAAGQRVNPESWTHGSAAQRTEWFTRGLR
                     EGTVGACDTFKTGR"
     gene            complement(2337..3386)
                     /locus_tag="PLB01_11370"
     CDS             complement(2337..3386)
                     /locus_tag="PLB01_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ77945.1"
                     /translation="MRAPSSLISRIPLPEESEGRIFVVDDDEQVRGLMRRLLTRSGYT
                     VEEFGVGATALEAIRERPPDLVLLDLQLPDASGNDVLAEIRENPATRLLPVVMMTGHG
                     TRDDRLRAQRTGVTDFLAKPVAADELLPRVKSLVLLKHFADEHEHAERVILMLAKTID
                     ARDSYTAGHSGRVAQYACDAAGKLGLKPAEIEDIRRGALFHDIGKIVVSDAVLKKVGP
                     LTPEERLTINQHPVVGCELLSGMKTMRAILPIVYHHHERLDGSGYPEGISGADIPLAV
                     RTVSVADVFDALTTTRAYRPAMTFEQAWKEIDGEVARGRFDPDPVNALKEVVAVTGIR
                     RDPGAPAAISGLFRY"
     gene            3602..6013
                     /locus_tag="PLB01_11375"
     CDS             3602..6013
                     /locus_tag="PLB01_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HQQ77946.1"
                     /translation="MKTGLRLLALAAASLAAAWVETAAAQSVPAALPALREAQIGEVW
                     ISQPTVAPASEAAAVPAAVGASARVFGEAGNQITFSFAPTGFASDGTAAAGWPPEKAA
                     ALSGFMNLMLPVLRDLYGPPATTYTVKVVRDLRYTASAVFFPSTDEIHAGDGVTYQLL
                     THELVHAFRRDRILSSGPQWQFDPTLSGFEEGFAQAVSYEAMTEFARRNPSFGLAQRV
                     YQSSNEWDYDFQNVPELRTRDFWSDSGGMLLYWTRYEMAAAAIEKIEREHPGFHRGFN
                     AEYFRRLNADPGLTPSRDLVKDIVRTVAPTIEGRPAALWIDQQYIFDCADHPGRKIWL
                     STQHYPAPADYFIFNRVFFYETFPNGSDWATPNAAGGYDYYHLNGAAGTAALHAGNGS
                     VVWQSALRITPSENPPSFYGFGNDNVDLTTQATNLPWPGGDPSRFATGLPFDLYRLNV
                     QFTSGTTVSSDSWRVIGSPIRNAAGVFGGVVGANGGTITLKHRNHAAEPPVPVVSGVF
                     WATPSWASLPHPETASVDTDPGVVDVTYVDAAGNTYTDVRTIGYGSAHGDQTFLFDIS
                     RMNAVPVLKPVSIVSQPSGRTATAGQSVPFTVAATGTPAPTYQWQVSTDAGASWADLA
                     DGGPYAGTRTATLVLAGVTLGMNGGRYRAVATNPLGSAVSAPAALAVSPSGQGATLFH
                     PLIPCRLFDTRHAEGSDAAAPALAAAATRILPLAGRCGVPPTAAAVSVNVTVVDPAAS
                     GSLVFYPGDQPVPIASTVSFRTGRTRANNAIIRIAADGSGTLGVSNDAAAPVPFILDV
                     NGYFE"
     gene            complement(6014..7372)
                     /locus_tag="PLB01_11380"
     CDS             complement(6014..7372)
                     /locus_tag="PLB01_11380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ77947.1"
                     /translation="MSRSASRALRIAVVDDDEGVRELLRSTLVREGATVDVYPGSQAF
                     LDRAVFTDYDVIFCDLMLPGLSGIDLLFEVRRKAPNVPFVIISGQAGVSDAIEAMKAG
                     ATDFLVKPFRIQSLLDVVRRLTPDTAPRTASGIWRRAKEPNAAVGTSAAWRDVLEKAR
                     RVAELSSTVLIRGETGTGKEVVAKYIASHGPRAGKPFVTLNCAAIPDDLIESELFGHV
                     RGAFSGATNARRGLFEEAEGGTLLLDEVGSMPVAAQAKVLRALEEFQIRRVGDNTNVA
                     VDVRIIASTNLDMEAAVRRQTFREDLYYRLAVVTLRVPPLREREEDKLALAEHFLKMF
                     SKPSQYPRRLSADARDLILRDPFPGNVRELKHAIEQACALSPREELAAEDFPLLLSRA
                     DAEGQGDAGSAAGASAVTPEQLLEALARTGGNRVEAARQLRISRSTLYRLLRRTAQEP
                     AP"
     gene            7477..8046
                     /locus_tag="PLB01_11385"
     CDS             7477..8046
                     /locus_tag="PLB01_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQQ77948.1"
                     /translation="MRNPAALALAAAAALLAGSGCSSVTTTVDYDPATNFGAYKTFAF
                     KDVHGRGQFQMKRVENAIERTLGAKGLVKMDAKADVKPDLWVVLHTKLKNDKQITTYN
                     SGWGWGWGWRGYGWGGGGWTTARITDVPVGTLVVDLVDTKEKELVWRGAAARDVAANE
                     TPQDRAEITQKAIDKLFEGFPPGVPPKTP"
     gene            complement(8056..>10120)
                     /locus_tag="PLB01_11390"
     CDS             complement(8056..>10120)
                     /locus_tag="PLB01_11390"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF025376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQQ77949.1"
                     /translation="DSTAHRAEQLARALRAAPEGTPDRAHEALTYLEWNPLVDARAEF
                     EETVADLLGPCSEVLLDYEGRQISPEWVDVPEKVVLALLRHGLVPGEDRHLLFRVPNP
                     WIEQDEEKISKILASVARANVLFLLACEKLGIAPARNAIHELSIPQVNARAEIGAVVK
                     IGTLYLEAAAGLFGGPPDRVDAFLSARVPAARRADVIARAARVRLVPLVENVGALAHL
                     PDFLEAYYQALERNVGVEGLPAPAGLRTGFDAKESVVRVFVAMSDTAEQSGKIATDAA
                     YTLTIAARDEAERRLAEHARRIGEPAPRVTFLIGAGRAGFRGGFDPEHEGVVAQFARA
                     DGVTLQGIRADAPEGTLRLAAKFREAAAAKRPAPAVGAGEREALLELLDAGVKAHTET
                     LLRIAPLLAPFSGLVPQTRVRIRATGSVNYGRSIPVYPEEWGGGRTLPDNRDLRDAWP
                     EGVTLPRAINYNLGCTTLGLPAVTSDLAALDKRAAVLLDRQVPGYREILASELPHFVR
                     EAVALVFGKKFAETAARRCLRAAAAVWADVRARAELVEPTCLFAMLYLRYMADDSDSW
                     DETKEHESLTTREEELIRETSSGAFGALCAEKPGGRWTILQTLVDSEEAPRLLLAREL
                     TIEEKREFVDLRIEGWLRTLPDPLARSLRAAWAPLRELGKDELTLRATEQMVALERLR
                     GSRGA"
BASE COUNT         1484 a         3644 c         3537 g         1455 t
ORIGIN      
        1 agcccgtaga cgtccgcgcg cgcgtcgacc ggcccgcccg acgcctgctc cggcgccatg
       61 aagccgggcg tgccggaggc gtccgcaggc cccgacgccg ccgcgcgggc gaggccccag
      121 tcgagcacga gcacctcgcc gaacggtccc accatgacgt tcgagggttt caggtcccgg
      181 tggacgactc cggccgcgtt cgcgaacgcg acgggctcgc acaccctgag gaagagcctc
      241 agccgttccg gcagagtcac ggcgcgcgcg gcggcggcgg cgaggccttc gccccgcacg
      301 agcttcatcg cgtagaacgc gcggccgtcg ggcagtcggc ccgcgtcgtg cacggggacg
      361 aggccggggt gctcgagccg ggcgagcacg cgggcttcgg ccagcagcgc ctccgccggc
      421 ccctcacctt cgagagacac gtccacgacc ttgagcgcga ggcgccgccc gagaatctcg
      481 tcctccacct cgaagacggc gcccatgccg ccggccccga tcgtcccggc gagccggtag
      541 cgcgtgccgg agaggtccgg cgcacgcacg gcctcccgca gccgcgcgac ggccgagtcg
      601 gggagcgtca cgaaggcggc tccacgccga gaagcgcgcg cgcctcggct cggaactccg
      661 cgtcggagac cgtgagctcg cgaaggacgt cgaggacgat gccgcggaac tcgcggcggg
      721 ccgccgcgag ctggttcgtg acgtcgacgg cggtcgtccc gaaccggagc gccaggtcct
      781 cgtaccgcgg acgctccccg gcctcgccgt cctcgaggtc gtacgccgag aagagcgcga
      841 accggctctc gtgaccggac gctgagcatc gctcccgcag gcgggccacg gccagctcga
      901 acacgctccg cgcccattcc ctctcgaaga gatcttcggg tgagagatcg ccggggccgc
      961 gccgcgcgtc gacctcgcga cgaacggctt cgaggtccag cgaaaggacc tcgcttccgc
     1021 ccccgcgctt caggcggctc cttgccttcc actcgttcgc ggcgagcccg tccacgagga
     1081 cgcgcaggta cgtccggagc cgcgccttcg acgggtcgaa ccgggacagc ccgtccttct
     1141 ccacgagcga cgcgaagaac tcctgggtca ggtcggccgc ctcctcgtgg gatcggccgg
     1201 ccctcagacg cacgtacgtg tagatgggct tccagtacgc ttccgcgagc gtcccgaggg
     1261 cgcgcgagcg cgcggcggca tcgccgcccg tgacggcggc gacgagagac ccgcgcgtgt
     1321 cgggaaaggg cgccatggag agcggagacg ctagcacgcg cccggaatcg catgcatggt
     1381 tccgcccttg cctatgcccc gctgagttga aagggcggca taagatcccc gccgacagga
     1441 gaagttcatg cgctggagca acgacggcgg cgggctcggc ccggacatcg aggaccgccg
     1501 gggcgaaggc ggaggcggct tcaacttcgg cggcggcggg ctgcgcctcg gctgcggcgg
     1561 gatcatcctg ctcggcgtcc tctccctcat cttcaagcag aacttcttcg ctctcctctc
     1621 cccgcagggc ggcgctccgc aggcgcgcgt ggactcgcgc cccgtggcgc cgccgtctcc
     1681 gaaggaagag gagcgcgcgc gcttcgtgaa cttcgtcgtg aacgacgtcc agaagacgtg
     1741 ggaccagaag ttccccggcc tcggccgcaa ctacacgcac gcgaagctcg tcctctaccg
     1801 cgacgccacg cgctcgggct gcggcttcgc gcaggcgcag atgggaccgt tctactgccc
     1861 cgccgaccgc aaggtctacc tcgacatggg cttcttcgac gagctctccc agcgcttcgg
     1921 cgcgccgggc gacttcgcgc aggcgtacgt catcgcgcac gagttcggcc accacgtcca
     1981 gacgctgctc ggcatcgagg gcggcgtccg ccgcgcgatg gcgcagaacc cctcgcagga
     2041 gaaggcgctc tccgtgcgcc tcgagctgca ggccgactgc ctcgcgggcg tctggggcca
     2101 cggcacggcc gagcgaaaca tcctcgagaa cggcgacgtc gaaaagggcc tcgccgcggc
     2161 ggccgccgtc ggcgacgacc ggctccagcg cgccgccggc cagcgcgtga accccgagag
     2221 ctggacgcac ggctcggccg cccagcgcac ggagtggttc acgcggggcc ttcgggaagg
     2281 caccgtcggg gcctgcgaca cgttcaaaac aggccgctga cgccgttcgc gcccggtcag
     2341 tacctgaaga ggcccgagat ggcggccggg gctccgggat cgcgccggat tcccgtgacc
     2401 gcgaccacct ctttgagggc attcaccggg tccgggtcga agcggccccg ggcaacttcg
     2461 ccgtcgatct ccttccacgc ctgctcgaac gtcatcgccg ggcggtaggc gcgcgtcgtc
     2521 gtcagcgcgt cgaacacgtc ggcgaccgag acggtgcgga cggccagcgg gatgtccgcg
     2581 cccgagatcc cctcggggta gccgctgccg tccagccgct cgtggtggtg gtagacgatc
     2641 ggcaggatcg cccgcatcgt cttcatgcct gacagaagct cgcacccgac gaccggatgc
     2701 tggttgatcg tgagccgttc ctcgggcgtc aggggcccga ccttcttgag caccgcgtcg
     2761 gaaacgacga tcttgccgat gtcgtggaaa agagcgccgc ggcggatgtc ctcgatctcc
     2821 gcgggtttca ggcccagctt tccggccgcg tcgcaggcgt actgcgcgac gcgtccgctg
     2881 tggccggccg tgtagctgtc gcgcgcgtcg atcgtcttgg cgagcatgag gatgacgcgc
     2941 tcggcgtgct cgtgctcgtc ggcgaagtgc ttgagaagga cgagggactt cacgcgcgga
     3001 agaagctcgt ccgcggcgac gggtttcgcg agaaagtccg tgacgccggt gcgctgggcg
     3061 cgcagccggt cgtcgcgcgt gccgtgaccc gtcatcatca cgacgggcag gagacgtgtg
     3121 gcggggtttt cacggatctc ggcgaggacg tcgttcccgc tcgcgtccgg gagctggagg
     3181 tcgagcagca cgaggtccgg cgggcgctcc cggatcgcct ccagcgcggt ggcgccgact
     3241 ccgaactcct cgacggtgta gcccgagcgc gtgagcagcc gccgcatgag gccgcgcacc
     3301 tgttcgtcgt cgtccacgac gaagatccgg ccttcggatt cttcgggcaa cgggatcctg
     3361 gaaatgagcg acgacggcgc gcgcatatgc aagggactat acgctcgcgt gactccgcca
     3421 ctccgtgctt cccacgggtg tgtctgacac cgttccgggc acagcgctga cacatcgacc
     3481 ggttccggag ccccgttaag cgctctgttt tcaagggtct ttcggcccgc gtcgagggcc
     3541 gggggcggcc ttcgggttgc aacgcgtcct gaagcgggaa accgcgcaaa gagacgcgca
     3601 catgaagacc ggactccgac tcctcgcgct tgccgccgcc tcgctcgcgg cggcctgggt
     3661 cgagacggcc gcggcgcaga gcgttccggc cgccctcccc gcgctgcgcg aggcgcagat
     3721 cggagaggtc tggatctcgc agccgacggt ggcgccggct tcggaggccg cggcggtccc
     3781 ggccgccgtc ggcgcatccg cgagggtctt cggcgaggcc ggaaaccaga tcacgttctc
     3841 gttcgcaccg acgggatttg cttcggacgg cacggcggcg gcgggctggc cgcccgaaaa
     3901 ggccgcggcc ctgagcggct tcatgaacct gatgctcccg gttctccgcg atctctacgg
     3961 gccgcccgcc acgacgtaca cggtcaaggt cgttcgcgat ctgagataca cggcttccgc
     4021 cgtcttcttc ccgtccaccg acgagatcca cgccggcgac ggcgtcacgt accagctgct
     4081 cacgcacgag ctcgtgcatg cgttccggcg cgacaggatc ctgagcagcg gaccgcagtg
     4141 gcagttcgac ccgacgctga gcggtttcga ggagggcttc gcccaggccg tgtcctacga
     4201 ggcgatgacg gagttcgcgc gccgcaatcc ctcgttcggg ctcgcgcagc gcgtctacca
     4261 gtcgagcaac gagtgggact acgacttcca gaacgttccc gagctccgga cccgggactt
     4321 ctggagcgat tcgggcggaa tgctcctcta ctggacccgt tacgaaatgg cagccgccgc
     4381 gatcgagaag atcgagcggg agcatccggg cttccaccgc ggcttcaacg ccgagtactt
     4441 ccgccggctc aacgcggacc cgggcctgac gccctcgcgc gatctcgtga aagacatcgt
     4501 ccgcaccgtc gctccgacga tcgaaggccg gcccgcggcg ctctggatcg accagcagta
     4561 catcttcgac tgcgccgatc accccggcag gaagatctgg ctttcgacgc agcactaccc
     4621 cgcgcccgcg gactacttca tcttcaaccg cgtcttcttc tacgagacgt ttccgaacgg
     4681 ttcggactgg gcgacgccga acgcggcggg cggctacgac tactaccacc tcaacggcgc
     4741 cgcgggaacg gcggcgctcc acgcgggcaa cggctcggtc gtctggcaga gcgcgctcag
     4801 gatcacgccc agcgaaaacc ctccttcctt ctacgggttc gggaacgaca acgtcgacct
     4861 cacgacgcag gccacgaatc tcccctggcc gggcggcgac ccttcccggt tcgccacggg
     4921 cctccccttc gatctctatc gcctgaacgt ccagttcacg tccggaacga cggtctcgag
     4981 cgactcatgg cgcgtcatcg gctcgccgat tcgcaacgcc gcgggagtct tcggcggagt
     5041 cgtcggcgcg aacggcggga cgatcacctt gaagcacagg aatcacgcgg ccgagcctcc
     5101 cgttccggtc gtgagcggcg tcttctgggc gacgccttcc tgggcgagcc tgccgcatcc
     5161 ggagacggcc agcgtcgaca ccgaccccgg cgtcgtagac gtgacctacg tcgacgcagc
     5221 cggcaacacg tacaccgacg tgcggacgat cggctacggc agcgcgcacg gcgaccagac
     5281 gttcctgttc gacatctcgc gcatgaacgc ggtgccggtt ctcaagcccg tctcgatcgt
     5341 ctcgcagccg tcgggccgga cggcgacggc cgggcagagc gtcccgttca cggttgccgc
     5401 gaccggaacg ccggccccga cgtaccagtg gcaggtctcg accgacgcgg gcgcttcctg
     5461 ggccgacctt gccgacggcg gcccttacgc gggcacgcgg acggcgactc tcgtgctcgc
     5521 gggcgtgacg ctcggaatga acggcggccg ctatcgggcc gtggcgacga acccgctcgg
     5581 ctcggcggtg agcgcccccg cggcgctcgc cgtgtcgcct tccgggcagg gcgccacgct
     5641 cttccatccg ctcattccct gccgcctctt cgacacccgc cacgccgagg ggtcggacgc
     5701 cgccgccccg gccctcgccg ccgcggcgac acgcatcctc ccgctcgccg ggagatgcgg
     5761 cgtcccgccg acggcggccg ccgtttcggt caacgtgacg gtcgtcgacc cggcagcctc
     5821 cggctccctg gttttctacc ccggagacca gccggtgccc atcgccagca cggtctcgtt
     5881 ccggacggga cggacgcggg cgaacaacgc gatcatcagg atcgcggcgg acgggagcgg
     5941 cacgctgggg gtatcgaacg acgcagccgc tccggttccg ttcattctcg acgtcaacgg
     6001 atacttcgag tgatcatggc gccggctcct gcgccgtcct gcggagcagc cggtagagcg
     6061 tggaccggct gatccgaagc tggcgcgccg cctccacgcg gtttccgccc gtccgggcga
     6121 gagcctccag cagctgctcg ggcgtcacgg cggaggcccc ggccgctgat ccggcgtcgc
     6181 cctggccttc cgcgtcggcg cgggagagga gcaacgggaa gtcctcggcc gccagctcct
     6241 cgcggggcga cagcgcgcac gcctgctcga tggcgtgctt gagctcgcgg acgttgccgg
     6301 gaaacggatc gcggaggatc agatcccggg cgtcggccga aagccgccgc ggatactggg
     6361 acggcttcga gaacatcttc aggaagtgct cggcgagcgc gagtttgtcc tcctcgcgct
     6421 cccggagtgg cggaacccgc agggtgacga ccgcgagccg gtagtacagg tcctcgcgga
     6481 acgtctggcg ccgtacggcg gcctccatgt cgagattcgt cgaggcgatg atccggacgt
     6541 ccacggcgac gttcgtgttg tctccgacgc ggcggatctg gaactcctcg agcgcgcgga
     6601 ggaccttggc ctgcgccgcg accggcatcg acccgacttc gtcgaggagg agcgttccgc
     6661 cctcagcttc ctcgaagagg ccgcgccgtg cgttcgtggc cccgctgaag gctccgcgca
     6721 cgtggccgaa gagctcgctc tcgatgaggt cgtccggaat cgccgcgcag ttgagggtga
     6781 cgaacggctt gccggcccgg gggccatgcg acgcaatgta cttcgcgacg acttccttgc
     6841 ccgtccccgt ctcgccccgg atcaggaccg tggacgagag ctcggcgacg cgcctcgcct
     6901 tctcgagaac gtccctccag gccgccgagg tcccgaccgc ggcgttgggt tcctttgccc
     6961 gtctccagat tccggatgcc gtccgggggg ccgtgtccgg cgtgaggcgc ctgaccacgt
     7021 cgagaaggct ctgaatccgg aacggcttga cgaggaagtc cgtcgccccg gccttcatcg
     7081 cctcgatcgc gtccgagacg ccggcctggc ccgaaatgat gacgaaggga acgttcggcg
     7141 ccttccggcg cacctcgaag agaaggtcga tacccgagag gcccggaagc atgaggtcgc
     7201 agaagatgac gtcgtagtcc gtgaagacgg cccggtcgag aaaggcctgg gagccgggat
     7261 agacgtcgac ggtcgcgcct tcgcgaacca gggtggaccg cagcagctcg cggacgccct
     7321 cgtcgtcatc gacgaccgcg atgcggagcg cgcggctggc ggatcgcgac acggcaggcc
     7381 attgtacggc ccgcccatgc gcgccggccg tcctctcggg gctgcgtgtc aacccggcaa
     7441 ggctcttgca cgtccatcct gcggagggac acacccgtga ggaatcccgc tgctctcgcg
     7501 ctcgccgccg ccgccgccct cctcgccgga tcgggctgct cgagcgtcac gacgaccgtg
     7561 gactacgacc ccgccacgaa cttcggcgcc tacaagacct tcgccttcaa ggacgtccac
     7621 ggccgcggcc agttccagat gaaacgcgtc gagaacgcga tcgagaggac gctcggcgcg
     7681 aagggcctcg tgaagatgga cgcgaaggcg gacgtgaagc ccgacctgtg ggtcgtcctc
     7741 cacacgaagc tgaagaacga caagcagatc acgacgtaca actcgggctg gggctggggt
     7801 tggggctggc gcggttacgg ctggggcggc gggggctgga ccacggcccg catcaccgac
     7861 gtccccgtgg gcacgctcgt cgtggatctc gtcgacacga aggagaagga gctcgtctgg
     7921 cgcggcgccg ccgcgcgcga cgtcgccgct aacgagacgc cgcaggaccg cgccgagatc
     7981 acgcagaagg cgatcgacaa gctcttcgag ggcttcccgc ccggcgtccc gccgaagacg
     8041 ccctgatccc ttctctcagg ccccgcggct gccgcggaga cgctccagag cgaccatctg
     8101 ctcggtcgcg cggagcgtca gctcgtcctt gccgagctcg cggagcggag cccacgcggc
     8161 cctgaggctc cgcgcgagcg ggtcgggcag cgtgcggagc cagccctcga tccggaggtc
     8221 gacgaactcg cgcttctcct cgatcgtgag ctcgcgcgcg agcaggaggc gcggcgcctc
     8281 ctcggaatcc acgagcgtct ggaggatggt ccagcgcccg ccgggcttct cggcgcagag
     8341 cgcgccgaag gcgccgctcg acgtctcccg gatgagctcc tcctcgcgcg tcgtgaggct
     8401 ctcgtgctcc ttcgtctcgt cccagctgtc ggagtcgtcc gccatgtagc ggaggtagag
     8461 catcgcgaag aggcacgtcg gctcgacgag ctccgcgcgc gcccgcacgt ccgcccacac
     8521 cgccgccgcg gccctgaggc agcggcgcgc ggccgtctcc gcgaacttct tcccgaagac
     8581 gagcgcgacg gcctcgcgca cgaagtgcgg aagctcgctt gcgaggatct cccggtagcc
     8641 cggcacctga cggtcgagga gcaccgccgc acgcttgtcg agcgccgcga ggtcgctcgt
     8701 gacggcgggg aggccgagcg tcgtgcagcc gaggttgtag ttgatcgccc gcggaagcgt
     8761 cacgccctcg ggccacgcgt cgcggaggtc ccggttgtcg ggcagcgtgc gtccgccgcc
     8821 ccactcctcg gggtagacgg gaatcgagcg gccgtagttc acggaacccg tcgcgcggat
     8881 cctgacgcgc gtctgcggga cgagcccgct gaacggcgcg aggagcggcg cgatccggag
     8941 gagcgtctcc gtgtgggcct tcacgcccgc gtcgagaagc tcgaggagcg cctcgcgctc
     9001 ccccgctccg accgcgggcg cgggccgctt cgcggcggcc gcctcgcgga acttcgcggc
     9061 gaggcggagc gtcccctccg gcgcgtcggc gcggattccc tgcagcgtga cgccgtccgc
     9121 gcgcgcgaac tgcgcgacga cgccctcgtg ctccgggtcg aagccgccgc ggaagccggc
     9181 gcgtcccgcg ccgatcagga acgtcacgcg gggcgcgggc tcgccgatcc ggcgggcgtg
     9241 ctccgcgagc cggcgctcgg cctcgtcgcg cgcggcgatc gtcagcgtgt acgccgcgtc
     9301 cgtcgcgatc ttgccgctct gctcggcggt gtcggacatc gcgacgaaga cgcggacgac
     9361 cgactccttc gcgtcgaagc ccgtccggag gccggcgggc gccgggagac cctcgacgcc
     9421 gacgttccgc tcgagcgcct ggtagtaggc ctcgaggaag tccggaaggt gcgcgagggc
     9481 gccgacgttc tcgacgagcg gcacgaggcg cacgcgcgcc gcgcgtgcga tcacgtccgc
     9541 gcgccgcgcc gccggcaccc gcgccgagag gaacgcgtcg acgcggtcgg gcgggccgcc
     9601 gaagaggccg gccgcggcct cgaggtagag cgtcccgatc ttgacgacgg cgccgatctc
     9661 cgcgcgcgcg ttcacctgcg gaatggagag ctcgtggatc gcgttgcgcg ccggggcgat
     9721 tccgagcttc tcgcacgcga gcaggaacag cacgttcgcg cgcgcgacgg acgcgaggat
     9781 cttcgagatc ttctcctcgt cctgctcgat ccacgggttc ggcacgcgga acaggaggtg
     9841 ccggtcctcg ccggggacga gcccgtggcg gaggagcgcg aggacgactt tctccgggac
     9901 gtcgacccac tccgggctga tctgccggcc ctcgtagtcg agcaggacct ccgagcaggg
     9961 cccgaggagg tccgcgaccg tttcctcgaa ctccgcgcgc gcgtcgacga gcgggttcca
    10021 ctcgaggtac gtcagtgcct cgtgcgcgcg gtcgggcgtc ccctcgggcg ccgcgcggag
    10081 cgcccgcgcg agctgctcgg cccggtgcgc cgtcgagtcc
//
LOCUS       DAOMFY010000131        10097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2038_length_10097_cov_7.501593, whole genome shotgun sequence.
ACCESSION   DAOMFY010000131 DAOMFY010000000
VERSION     DAOMFY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2038_length_10097_cov_7.501593"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PLB01_11395"
     CDS             complement(<1..855)
                     /locus_tag="PLB01_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQQ77950.1"
                     /translation="MSAPAAAAVVASIGRLVATVRTAYRGPAAAVEDAVVCLLARGHL
                     LIEDVPGVGKTTLATALGKALGVGVHRLQFTPDTLPSDILGLTVFNQRAQEFEFHPGP
                     IFTPVLLADEINRATPKTQSALLQAMNERAVSSDGVTRPLPEPFLVLATQNPVEYLGT
                     YPLPESQLDRFLLRISLGYPAPDEERRLLISGGADRVLAQLQPAMNLEDLKAAQDATE
                     RVTVADKLMDYILAIVARTRTGKDLLLGVSPRGAQGFFRAVQARALVAGRPYATPDDV
                     KRVAVPALA"
     gene            complement(852..1967)
                     /locus_tag="PLB01_11400"
     CDS             complement(852..1967)
                     /locus_tag="PLB01_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQQ77951.1"
                     /translation="MADASVRALILAGGSGTRLWPRSTDSKPKPFLALTGRDSLLRET
                     YRRAAAVAGAANVWVSGRASHGALLAAELPEVPASRLVLEPTRRNTAPAIALSVLAAC
                     ADDPRAVVLVLPSDQAVADEPAFLAALREGAAVAAKEDAFVTLGIPPTRPETGYGYLE
                     TFEEHEETGEKISSSGGEQGGRTARGEAPAARVVRRFVEKPDAAKAAEFLASGRFLWN
                     AGIFLFRAEHLLAEMQRTCPEVLAAARRAFAARASGDHTTFKESFSSSPSISIDVAVM
                     EKARRVLTIPSACGWSDLGSWEAVFEFRGGRPGADVIEGDGASEGGAGNLVLADARPV
                     RVVGLSGVAVVESPDGLLVMKLGASDDLRRAVEKGLK"
     gene            complement(2090..3004)
                     /gene="murB"
                     /locus_tag="PLB01_11405"
     CDS             complement(2090..3004)
                     /gene="murB"
                     /locus_tag="PLB01_11405"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQQ77952.1"
                     /translation="MIPDALRGPGIAVREGEPLAARTTLRIGGPARFFAEVADAEALA
                     RVLRFASAEGLPLLVLGKGSNLLVPDAGFPGVVVVLGGTFRAVRVEGAEVVAGGGVSL
                     MALAVAARDAGLSGVENVSGIPSSVGGAVRINAGSYGSEIFDVLVSATLVSRAGEARA
                     VAATEIPHGYRWTALCERDDVVAEARFRLSKKSPAEIAERMAEVAARRRDALPKQPNA
                     GSIFKNPPGLFAGKLLEECGLKGRRVGGAEISPVHANVIVNTGGATAEDVKALMTEMK
                     KAVKGRFGIELEPEIQIVGEEKISLKGE"
     gene            complement(3013..3483)
                     /locus_tag="PLB01_11410"
     CDS             complement(3013..3483)
                     /locus_tag="PLB01_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HQQ77953.1"
                     /translation="MTTLVSTWFGAGFLPIAPGTWGSLATIPVAHLIVLLWGVWGLAG
                     FALGVAIVGTHASGETARLRGVKDPSEVVVDEVAGQSLALLPVYALVPPDAVVLRIGT
                     ILLAFLLFRILDVWKPGPIGWLERLPGGFGIMADDLLGGAVAAAMVAAGLLLKG"
     gene            3784..4863
                     /gene="recA"
                     /locus_tag="PLB01_11415"
     CDS             3784..4863
                     /gene="recA"
                     /locus_tag="PLB01_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011647718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQQ77954.1"
                     /translation="MNKEKLKALEQAMQQIDRQFGKGALVRLGDKPQETIQAISTGSI
                     GVDYALGIGGVPRGRVLEIFGPESSGKTTLTLHIIAEAQKRGGLAAFIDAEHALDPDY
                     ARKLGVDVDNLMVSQPDSGEQALEIAEALVRSAAVDVVVVDSVAALVPKAELEGEMGD
                     AMVGLQARLMSQALRKLTAVVSKSKTTLIFINQIREKIGVMFGNPETTTGGRALKFYS
                     SVRIDIRRINAIKDGENVVGSRTKVKVVKNKVAPPFKVAEFDIGYGEGISKTGELVDL
                     GIEMKIVDKSGAWFSYGDLRIGQGRENTKQFFRDNPDIATEIEKKIRTNLGLPASDVF
                     AAGPVTVATPHPSAEPKSGKGFGKK"
     gene            4960..5385
                     /locus_tag="PLB01_11420"
     CDS             4960..5385
                     /locus_tag="PLB01_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77955.1"
                     /translation="MTRRAALEFWSRSVSTFSLFATSVVALTTAFHGAPFPLDTALEG
                     LALVGVLPAVWVALARLAERRYLSRPPKAFLAASLGLDCGGLLLVAAAIALLQSHALD
                     AGTLLRAGGVLEGCGTVLLVAWFVAVLAVEPSGDHMRGL"
     gene            5412..8390
                     /locus_tag="PLB01_11425"
     CDS             5412..8390
                     /locus_tag="PLB01_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77956.1"
                     /translation="MKVHRLLAVLLGGLLSPAAAGETPRPYVVPWLAVGPRDARIARI
                     ANLPTAARSANAPGLLLRLDAAGWAGGRAAAVEASRELAEHAHGAGWKWGLKLELPDA
                     AVPADVRAAEAATVEDLWPGLGEILRDAKTADLVTISFSAPAGADSKARAYLLRKCTS
                     GARAAAPHARIVFEAPPGAAGDLVSAEARLLLSEENTAYLDLIAVAGNERTTPAQVRG
                     AIDSVAFGKPGLVDLGGSVRTPGAVLAAATRLAPENIPFVTVSPAWAPADDAILERFG
                     RFVSGDFGPDTRAASVAAADGSPRDAFRVVSGADLGGVVLVSGTRDGSPADTGAFTLT
                     LDATPYASAEILELATGATKRLEIPASAVPARLTLSLAKGPLAVRLTAREKAPAEAPT
                     AAVGVTAARGITAEEILARHQAWRAARDARWKTLSATNSLSMRFRFANLGNTLDLTLA
                     GPFFYEKGAGFDWAWHDAYFNGVRWRAKTLPELPLLQPEKVSDMPLALTLDDAYRYTL
                     IGEDTVSGVACWSLDFEPRVAVTDKPSYAGRVWIARDGYGAVRIRTRQLNLTGEIQAV
                     DETSDFTDVPAPDGGPPLRFPTRTTGQWILKTFSRTTVIERESVLTDVRLDDATFRAE
                     REEAFASKDTMVRDTEKGVRYLEKTKDGGREVTEDRKRGHLFGLAGLFYDGSYDYPLP
                     LLGVYWIDLDASKRHDQVQVLFGGVLLAASWSQPRLFGSPVDVGVDVFGIAVRGTDAV
                     FVDGAEDESQRVKSRSFAAAFKAGVPLTRHLKLTGTVSESHRDFAADEDVTSPAFAIP
                     SDHWLTRVEGQLAWDWRGWALTGRWAWGKRSQWDAWGFAGNPDYSPDKDSFTTWGAEL
                     QKDFHLPRFQRIRTSVGYLGSDNTDRFSKYTFGFYGGTSLRGFRSGALRAEEAITSKF
                     AYGYVIGSAFRLEAIYEDARVTDRCAGLSWAYFSGAGISGEFVGPWATLIRFDAGTPV
                     AGRNRGYGGVVLSLNFLKIF"
     gene            complement(8387..9871)
                     /locus_tag="PLB01_11430"
     CDS             complement(8387..9871)
                     /locus_tag="PLB01_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77957.1"
                     /translation="MDTRRIVALAALGTLGGAAALAVFLAERPMAPGARFRPAAVPGE
                     DFALLPESGRTFARVVIGLRPHGTSRKNASRGARNPRAVELRRRLFGIDFELLHGACM
                     SAAPAYSTFFVAVPDPATVLEASGEEEADFRSYLESRLGWPEAAIRSRVRFFRVSRPL
                     LYPQDMAEILGLDTAGRLVLGVGADTDLQYIEPVETLVREFPRDFALRRLAGLHVGDV
                     NTEGGDLALSWLPDGKVGLLVGRHRAVRYLERLTGSEILGRPLTEAEIDESRRAFSLA
                     FFGVEVVIAGEAALRDPSRGSEELFHADMVVAAVRNDHEALAFVPTYVMRPRDANSGE
                     LFPEAFVRDVQREYDLVAGQMEGRGYRVVRLPFEDHPVRAPVNVSKFVDRTNGRPVVL
                     LGRYPEHRPAAPGEASPMLRLADAVVRLQSFVDAWEERPEEPRWAALQTEIENAWKQI
                     DAATASPNDLFEAQRALYEANGISVVPLPLYPSGEGGVHCLLLR"
BASE COUNT         1525 a         3625 c         3500 g         1447 t
ORIGIN      
        1 cgcgagcgcc gggacggcga cgcgcttcac gtcgtccggc gtcgcgtacg ggcgccccgc
       61 gacgagggcg cgcgcctgca cggcgcggaa gaagccctgc gcgccgcgcg gcgaaacgcc
      121 gagaaggagg tccttccccg tgcgcgtcct cgccacgatg gcgaggatgt agtccatgag
      181 cttgtccgcg acggtgacgc gctcggtcgc gtcctgcgcg gccttcaggt cctcgaggtt
      241 catcgcgggc tggagctgcg cgaggacgcg gtccgcgccc ccggagatca ggaggcggcg
      301 ctcctcgtcc ggcgccgggt agccgagcga gatccggagg aggaaccggt cgagctgcga
      361 ctcgggaagc gggtacgtcc cgaggtactc gacggggttc tgcgtcgcga ggacgaggaa
      421 cggctccgga agcggccgcg tcaccccgtc cgaggacacg gcgcgctcgt tcatggcctg
      481 caggagcgcg gactgcgtct tcggcgtcgc gcggttgatc tcgtcggcga ggaggacggg
      541 cgtgaagatc ggccccgggt ggaactcgaa ctcctgggcc cgctggttga agaccgtcag
      601 gcccaggatg tcgctcggga gcgtgtccgg cgtgaactgc agccggtgga cgccgacgcc
      661 gagcgccttt ccgagcgccg tcgcgagcgt cgtcttgccg acgccgggca cgtcctcgat
      721 gaggaggtgg ccgcgggcga ggagacagac gacggcgtcc tcgacggccg cggcggggcc
      781 gcggtacgcg gttcggaccg tcgccacgag gcgtccgatc gacgcgacga ccgcggcggc
      841 tgcgggagcg ctcatttcaa ccccttctcg acggcccggc gcaggtcgtc cgaggcgccg
      901 agcttcatca cgagcagtcc gtccggagat tccacgaccg cgacgcccga gaggccgacg
      961 accctcacgg gccgcgcgtc cgcgaggacg aggttgcccg cgccgccttc cgaggcgccg
     1021 tcgccttcga tcacgtccgc gcccggcctg ccgccccgga actcgaacac ggcttcccag
     1081 gatccgaggt cgctccagcc gcacgccgac ggaatcgtga ggacgcgcct cgccttttcc
     1141 atgaccgcga cgtcgatcga tatggagggg gaagaagaga aagattcttt gaaggtggtg
     1201 tgatcgccgc tcgctcgagc ggcgaatgcg cggcgggccg cggcgaggac ttcgggacag
     1261 gtgcgttgca tctccgcgag gaggtgttcc gcccggaaga gaaagatccc ggcattccac
     1321 aggaagcggc cggaggcgag gaactcggcg gcctttgccg cgtcgggctt ctcgacgaac
     1381 cggcgaacga cgcgcgcggc cggcgcttcg ccgcgcgccg tcctgccccc ctgctctcct
     1441 ccactcgaag aaatcttctc tccggtctct tcgtgctctt cgaacgtctc caggtagccg
     1501 tatcccgtct cgggacgagt cggcgggatg ccgagggtga cgaaggcgtc ctctttcgcg
     1561 gcgacggccg cgccctcgcg gagcgcggcg agaaacgcgg gctcgtcggc gacagcctga
     1621 tcggaaggca ggaccaggac gacggcccga ggatcgtccg cgcatgccgc gagcacggac
     1681 agcgcgatcg cgggcgccgt gttgcggcgc gtcggctcga gaacgagccg cgacgcggga
     1741 acctcgggca gttcggccgc gaggagcgcg ccgtgcgacg cgcgcccgga gacccagacg
     1801 ttcgcggcgc ccgcgacggc cgccgcccgg cggtacgtct cgcgcagcag cgagtccctt
     1861 cccgtgaggg cgaggaacgg cttgggcttc gagtccgtcg accgcggcca gaggcgcgtg
     1921 ccgctgcctc ccgcgaggat cagggcgcga acggacgcgt cggccatcga gacattgtac
     1981 ggaggccggg gattcggaga ttttcggatt cggattcgga tcttcttaga aggtgatccc
     2041 tgccgcgcag cgctcgagtt ctggcgagcc gggtgcaggc gccccgggct cactcaccct
     2101 tcaaagagat cttctcttct ccgacgatct ggatctcagg ttcgagctcg atgccaaacc
     2161 tgcccttcac ggccttcttc atttccgtca tcaaggcctt cacgtcctcc gccgtcgcgc
     2221 cgcccgtgtt cacgatgacg ttcgcgtgga cgggcgagat ctccgcccca cccacccgcc
     2281 ggcccttgag gccgcactcc tcgaggagct tcccggcgaa gaggcccggc gggttcttga
     2341 agatcgaccc ggcgttcggc tgtttcggaa gggcgtcgcg gcgcctcgcg gccacctcgg
     2401 ccatccgctc ggcgatttcg gccggagatt tcttcgaaag gcgaaagcgc gcttcggcca
     2461 ccacgtcgtc gcgctcgcag agcgccgtcc agcggtagcc gtgcggaatc tccgtggcgg
     2521 ccacggcccg cgcctcgccc gcgcgcgaga cgagcgtcgc ggagacgagt acgtcgaata
     2581 tctccgagcc gtaggagccc gcgttgatcc gcacggcgcc gccgacggac gacgggatgc
     2641 ccgagacgtt ctcgacgccg gagagccccg cgtcccgcgc cgcgaccgca agcgccatca
     2701 gggagacgcc gccgcccgcg acgacctctg ctccctcgac gcgaaccgcg cggaaggttc
     2761 ctccgaggac gacgaccact cccgggaatc ccgcgtccgg aacgagcagg ttcgagccct
     2821 tgccgaggac gaggagcggc agcccctccg ccgacgcgaa gcgcaggacg cgcgcgagag
     2881 cctccgcgtc ggccacctcg gcgaagaacc gcgcggggcc gccgatgcgg agcgtcgtgc
     2941 gcgcggcgag cggctcgcct tcgcggacgg cgatccccgg gccgcgcagg gcgtcgggga
     3001 tcatcccttc aatcatccct tcaggaggag accggccgcg accatcgcgg ccgcgacggc
     3061 gccgccgagg aggtcgtcgg ccatgatccc gaaaccgccc ggcagccgct cgagccatcc
     3121 gatcgggccc ggtttccaca cgtcgaggat gcggaagaga aggaaggcga ggaggatcgt
     3181 gccgatccgc aggaccacgg catcgggcgg gacgagcgcg tagacgggaa ggagcgcgag
     3241 cgactgcccg gcgacctcgt ccacgacgac ctcggacggg tccttcacgc cgcgcaggcg
     3301 ggcggtttcg ccggaggcgt gtgtcccgac gatggcgacg ccgagcgcga agcccgcgag
     3361 gccccagacc ccccacagga gcacgatcag gtgcgcgacc gggatcgtgg cgaggctccc
     3421 ccacgtcccg ggagcgatcg gaaggaaccc cgcgccgaac cacgtcgaga cgagcgtcgt
     3481 cacgcgcgcc tccgcgaaca cctcgcgcca ggggcgaaga attccttgcg tttcggattt
     3541 tccgggccta atttccatcc gttccttctg cctgcggcct catcctaggc cgtttcgccc
     3601 caagaagatt ttcttagaag gtgaatgcgg gctgtgccaa gtgcgcccgc ctcgccgttc
     3661 ccgcggcaag ccgccctcat tcacccttct aagaaatccg aattccgaaa tccgaatccg
     3721 agccgaatct cccttcttcc atccctataa tccgccacgc ttgaaggaga ccacatggtc
     3781 gaagtgaaca aggaaaaact caaggcgctc gagcaggcga tgcagcagat cgaccgccag
     3841 ttcggcaagg gcgcgctcgt ccgcctcggc gacaagccgc aggagacgat ccaggccatc
     3901 tccaccggct ccatcggggt cgactacgcc ctcggcatcg gcggcgtgcc ccgcgggcgc
     3961 gtcctcgaga tcttcgggcc cgagtcctcg ggcaagacga cgctcacgct ccacatcatc
     4021 gcggaggcgc agaagcgcgg cggcctcgcg gcgttcatcg acgccgagca cgcgctcgac
     4081 cctgactacg cccgcaagct gggcgtcgac gtcgacaacc tcatggtctc gcagcccgat
     4141 tccggcgagc aggcgctcga gatcgccgag gccctcgtgc gctcggcggc cgtggacgtc
     4201 gtcgtcgtcg actcggtcgc ggcgctcgtc ccgaaggccg agctcgaggg cgagatgggc
     4261 gacgcgatgg tcggcctgca ggcccgcctc atgtcgcagg cgctccggaa gctgaccgcg
     4321 gtcgtctcga aatccaagac cacgctcatc ttcatcaacc agatccgcga aaagatcggc
     4381 gtcatgttcg gcaaccccga gacgacgacc ggcggccgtg cgctcaagtt ctactcgtcc
     4441 gtgcgcatcg acatccgccg catcaacgcg atcaaggacg gcgagaacgt cgtcggcagc
     4501 cggacgaagg tcaaggtcgt caagaacaag gtcgcgccgc cgttcaaggt cgcggagttc
     4561 gacatcggct acggcgaggg gatctcgaag accggcgagc tcgtcgacct cggcatcgag
     4621 atgaagatcg tcgacaagtc gggagcgtgg ttcagctacg gcgacctgcg gatcggacag
     4681 ggccgcgaaa acacgaagca gttcttccgg gacaacccgg acatcgcgac cgagatcgag
     4741 aagaagatcc ggacgaacct gggccttccg gcgtccgacg tcttcgccgc gggacccgtg
     4801 acggtcgcca caccccatcc gtcggcggag ccgaaatccg gcaaaggatt cggaaagaaa
     4861 tgagctgagc cggcaaactt tttcgcgggg gctgcgtcca acgactgcat gcagctcctg
     4921 cgtatgcccg gatccgtgtc cgccagcgga cgatcgccgt tgacccgccg cgccgccctc
     4981 gagttctgga gccgctcggt ctcgacgttc tcgctcttcg cgacgagcgt cgtcgccctc
     5041 accacggcct ttcacggggc acctttcccg ttggacacgg ccctcgaggg gctggccctc
     5101 gtcggggtgc tgcccgccgt ctgggtcgcc ctcgcgcggc tcgccgagag gcgctacctg
     5161 agccgtcccc cgaaggcgtt cctggccgcc tccctgggtc tcgattgcgg tggattgctg
     5221 ctcgtggcgg ctgcgatcgc ccttctgcag tcacacgcgc tggacgccgg cacgctgctg
     5281 cgcgccgggg gcgtgctcga gggctgcggg accgtgctcc tcgtcgcctg gttcgtcgcc
     5341 gtgctcgcgg tcgagcccag cggcgaccac atgcgcggac tctgacgagg ttccgcccct
     5401 agaatgccgg ggtgaaggtg caccggcttc tcgcggtcct ccttgggggt ctgctctccc
     5461 ccgcagccgc gggggagacg ccccgtccgt acgtcgtccc gtggctcgcc gtcggtcctc
     5521 gagacgcgcg catcgcgcgc atcgcgaacc tgccgaccgc ggcccgttcg gcgaacgccc
     5581 cgggactcct gctccgtctg gacgctgccg gctgggccgg aggccgtgcg gcggccgtcg
     5641 aggcgtcgcg cgagctcgcc gagcacgcgc acggcgccgg ctggaagtgg ggcctgaagc
     5701 tggaactgcc ggacgccgcc gtgccggcgg acgtgcgtgc ggccgaggcg gcgaccgtgg
     5761 aggacctgtg gccgggattg ggagagatcc tgagggatgc gaagaccgcg gatctcgtga
     5821 cgatttcctt ctcggcgcct gcgggcgccg actcgaaggc gcgggcgtat cttcttagaa
     5881 agtgcacctc gggcgcgcgc gcggccgccc cgcacgcgcg gatcgtgttc gaagcgcctc
     5941 ccggcgccgc gggcgacctc gtgagcgccg aggcccgtct cctcctttcg gaagaaaaca
     6001 cggcttatct cgacttgatc gccgtggccg gaaacgaacg gacgactccc gcccaggtcc
     6061 gaggcgccat cgactcggtc gccttcggaa agccggggct cgtggacctc ggcggctccg
     6121 tccgcacccc cggcgcggtt ctcgccgccg cgacccggct cgcgcccgag aacatcccct
     6181 tcgtcaccgt ttcgcccgcg tgggcgcccg ccgacgacgc catcctcgag cggttcgggc
     6241 gcttcgtttc aggcgatttc gggcccgaca cgcgcgcggc ctccgtcgcc gcggccgacg
     6301 gctcgccgcg cgatgcgttc cgtgtcgtgt cgggcgccga cctcggcggc gtcgtcctcg
     6361 tgtccgggac ccgcgacgga tcgccggccg acaccggcgc cttcaccctg acgctggacg
     6421 cgacgcccta cgcctccgcc gagatcctcg agctcgcgac gggagccacg aagaggctcg
     6481 agatccccgc ctcggcggtc ccggcgcgcc tgacgctctc cctcgcgaaa ggccccctcg
     6541 ccgttcgcct gaccgcgcgg gagaaggcgc cggccgaggc tccgacggcg gcggtcggcg
     6601 tcacggcggc gcgtggcatc acggccgagg agatcctcgc ccggcaccag gcgtggcgcg
     6661 ccgcgcgcga cgcgcgctgg aagacgctct cggctacgaa ctcgctctcg atgcgcttcc
     6721 gcttcgcgaa ccttggcaac acgctcgacc tcacgctcgc cgggccgttc ttctacgaga
     6781 agggggcggg gttcgactgg gcgtggcacg acgcgtactt caacggcgtt cgctggaggg
     6841 ccaagacgct ccccgagctg ccgctcctgc agcccgagaa ggtctcggac atgccgctcg
     6901 cgctcacgct cgacgacgcg taccgctaca cgctcatcgg cgaggacacc gtcagcggcg
     6961 tggcctgctg gtccctcgac ttcgagccgc gcgtcgccgt gacggacaag ccctcgtacg
     7021 cgggccgcgt gtggatcgcc cgggacggct acggggccgt gcggatccgc acccgccagc
     7081 tgaacctcac gggcgagatc caggccgtgg acgagacgtc ggacttcacc gacgtcccgg
     7141 cgcccgacgg cgggccgccc ctccgcttcc cgacgcgtac gacgggccag tggatcctga
     7201 agacgttctc ccgcacgacc gtgatcgagc gcgagagcgt cctcacggac gtacgcctcg
     7261 acgacgcgac gttccgcgcc gagcgcgagg aggctttcgc gtcgaaggac acgatggtcc
     7321 gcgacaccga gaagggagtc cgctacctcg agaagacgaa ggacggcggc cgcgaggtca
     7381 cggaagaccg caagcgcggg cacctcttcg gcctcgccgg ccttttctac gacggctcgt
     7441 acgactaccc gctcccgctc ctcggcgtct actggatcga cctcgacgcg agcaagcgtc
     7501 acgaccaggt gcaggtcctc ttcggcggcg tcctcctcgc ggcgtcgtgg agccagccgc
     7561 gcctcttcgg gtcgcccgtc gacgtcgggg tcgacgtctt cggcatcgcg gtccgcggca
     7621 cggacgccgt cttcgtcgac ggcgccgagg acgagtcgca gcgcgtgaag agccgctcct
     7681 tcgcggccgc gttcaaggcg ggcgtgcctc tcacgcgcca cctcaagctg acggggacgg
     7741 tgagcgagtc gcaccgcgac ttcgcggccg acgaagacgt cacgtcgccc gccttcgcca
     7801 tcccgtcgga ccactggctc acgcgcgtcg agggccagct cgcgtgggac tggcgcggct
     7861 gggcgctcac gggccgctgg gcgtggggaa agcgatcgca gtgggacgcg tggggcttcg
     7921 cgggcaaccc cgactactca ccggacaagg actccttcac gacgtggggc gccgagctgc
     7981 agaaggactt ccacctgccg cgcttccagc gcatccggac ttccgtcggc tacctcggct
     8041 cggacaacac ggaccgcttc tcgaagtaca cgttcgggtt ctacggcggc acctcgctga
     8101 gaggcttccg ttccggcgcc ctgcgcgccg aggaggcgat cacctcgaaa ttcgcctacg
     8161 ggtacgtgat cgggagcgcg ttccgcctcg aagcgatcta cgaggacgcg cgggtgacgg
     8221 accggtgcgc cggcctctcc tgggcctact tcagcggcgc cggaatctcc ggcgagttcg
     8281 tcgggccctg ggcgacgctg atccggttcg acgccgggac tcccgtcgcg ggccggaacc
     8341 gcggctacgg cggcgtcgtt ctctccctga acttcctgaa gatcttctag cgcagaagca
     8401 ggcagtggac cccgccttcg ccgctcgggt agagcggcag cgggacgacg gagatgccgt
     8461 tcgcctcgta gagggcgcgc tgcgcctcga acagatcatt cgggctcgcc gtggcggcgt
     8521 cgatctgctt ccacgcgttc tcgatctccg tctggagcgc cgcccaccga ggctcctccg
     8581 gccgctcctc ccaggcgtcg acgaacgact ggagccggac gaccgcatcg gcgagccgca
     8641 gcatggggct cgcctcgccc ggggctgccg gccggtgctc cggatagcgt ccgaggagga
     8701 cgacgggtct cccgttcgtc cgatccacga acttgctcac gttcacgggc gcccggacgg
     8761 ggtggtcctc gaagggaagg cggaccacgc gatacccccg tccctccatc tggcccgcga
     8821 cgaggtcgta ctccctctgg acgtcccgca cgaacgcttc cgggaacagc tcgcccgagt
     8881 tcgcgtcgcg cggccgcatc acgtacgtgg gaacgaacgc gagcgcctcg tgatcgttgc
     8941 ggacggccgc gacgaccatg tcggcgtgga acagctcttc gcttccgcgc gacgggtcgc
     9001 gaagcgcggc ctccccggcg atcacgacct cgacgccgaa gaacgccaac gagaacgccc
     9061 ggcgcgactc gtcgatctcc gcctccgtca agggccggcc caggatctcg ctgccggtca
     9121 agcgttcgag atagcggacg gcgcggtggc gcccgacgag gaggccgacc ttcccgtcgg
     9181 ggagccagct caacgcgagg tcgccgccct cggtattcac gtcgccgacg tgcaggccgg
     9241 cgaggcggcg tagggcgaag tcccggggga actcccggac gagtgtttcg acgggctcga
     9301 tgtactgcag gtccgtgtcc gcgccgacgc ccaggacgag ccggcccgcc gtgtcgaggc
     9361 cgaggatctc ggccatgtcc tgggggtaga ggaggggacg cgagacgcgg aagaagcgca
     9421 cgcgggaccg gatcgccgcc tccggccagc cgagacgcga ctcgaggtac gaacggaaat
     9481 cggcctcctc ctcgcccgac gcctccagga cggtcgcggg gtcggggacg gcgacgaaga
     9541 acgtggagta agccggcgcc gcgctcatgc acgcgccgtg gagaagctcg aagtcgatgc
     9601 cgaagagacg ccggcggagc tcgacggcgc gagggttccg ggcgccgcgt gacgcgttct
     9661 tccgggaggt gccgtggggc cgaagcccga tgacgacccg ggcgaacgtg cgccccgatt
     9721 cgggcagcag ggcgaaatcc tctcccggca cggctgccgg ccggaaccgc gcgcccggag
     9781 ccatgggcct ctcggcgagg aagacggcga gcgccgccgc accgccgagc gttccaaggg
     9841 ccgcgagcgc cacgattcga cgcgtgtcca tccgcctgat cccgtgtcca ccgagtctac
     9901 gcgcggcccc gtacaatccg cccgccatga agagccgcgc gttctccgcc gcactcgccc
     9961 tcgccgtcgc cgcccccgcc gcccgcgcgg ccgaaccggt ccgctggacc gtcgcagacg
    10021 aggcccggat cgcggccgag gccgcgtcct gggccccgcc ggacttcaag cgccagctcg
    10081 cgaagcacgg aaagagg
//
LOCUS       DAOMFY010000132         9987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2061_length_9987_cov_8.299940, whole genome shotgun sequence.
ACCESSION   DAOMFY010000132 DAOMFY010000000
VERSION     DAOMFY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2061_length_9987_cov_8.299940"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLB01_11435"
     CDS             complement(<1..597)
                     /locus_tag="PLB01_11435"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQQ77958.1"
                     /translation="MKRPKNVHPATPAALRAAATRLRKGELVAFPTETVYGLGANALD
                     AKAVAKIFAKKGRPHDNPVIVHVSDLKMLRRVIRQMPPLATRLAARFWPGPLTLILSK
                     NSSIPSSVTAGLDTVAVRMPEGLARDLVRLAGVPIAAPSANLSGRPSPTTAAHVAEDF
                     PDVYVLDGGPTAHGVESTVLALEPHPVVLRQGALSEELL"
     gene            complement(633..1922)
                     /locus_tag="PLB01_11440"
     CDS             complement(633..1922)
                     /locus_tag="PLB01_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ77959.1"
                     /translation="MSSVALAVAPPVVAERRLSWGAKIGILWEVVIGGFIELWAHKVR
                     SLLTLTLLMLGVFALVVMTSVLDGVMDKVSIGFSGMSWDGTVAIQPKAPETTEEQKRF
                     AMSPGLRYEDLSRVAAPHPAAVAFLPRATKSTRIRTAGGPERIWVTGLTPDYALLMNR
                     PIASGRGLTEDDRKRRSAVAVVGSTLGVKLFGGADPVGRDVLVDGIPFRIVGLQAPVQ
                     IFNEELWIDSNGLMIPLETYMDRMDASHKLTSVAVKLRKRSDLDEVRALVLGRTKQAH
                     HGIEDVEIKDLESEAAKSYAGFLEQMYGWRVVLSSLAATVLLVGGVGVLSVMLISFSD
                     RRYEIGLRKSLGASDGEIFVQFLLEACVLAALGALLGTIGGAVLCKALSSKFPYGLVV
                     NPMGLVTAWAVALGLALVFGLYPAIRASRLSPMEAMR"
     gene            complement(1915..3162)
                     /locus_tag="PLB01_11445"
     CDS             complement(1915..3162)
                     /locus_tag="PLB01_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ77960.1"
                     /translation="MFSGAFRERFFGAWVEIRENLGRSTLQTLGVVLGVASVLGGFSI
                     SDSQRKRSDERWVKLGGMDRLNVQPSQAVKDGQPTALQTANLGLRNEDAVEGDALDQK
                     ATSGVAQQKNARARVRSAFADQDRQITGIGGDYLALNGYTVEKGRGFAADDFEKGQAV
                     AILGAEAVSTFFPTGDAVGQTIRVGDIPVVVVGTFAEKVFRFRENQGNQFWWRNRIIA
                     VPATLVAQRMSGDRWQRVDRVVFRMRDLNVGEKLTEGLTGLLLGNHRQQEDFRIDDIA
                     ARIRKRRSQGDVYNIIFMLSGILSLIGGGMVNVNIQMASLKERVREVGVKMAIGASGP
                     EIFKGFMTEALVLTVFGAVAGLLGGIAFSKAITSFLQIPLYMQPASFLWAFVLAAGFG
                     FLFALYPAWKASRLSPMEALRYE"
     gene            complement(3162..3860)
                     /locus_tag="PLB01_11450"
     CDS             complement(3162..3860)
                     /locus_tag="PLB01_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ77961.1"
                     /translation="MTTDRAIIETAGLTKVFGSNGMAVHALRGIDLTVARGEFVALVG
                     PSGSGKSTLMAIIGCLDSPTDGTYALDGVAVQGLSGTALARIRNEKIGFVFQSYNLLP
                     KASIVRNVELPLLYAGMSRKDRRRRALELLEQVGIPEKANVLPAQLSGGQKQRVAIAR
                     ALANTPALLLADEPTGALDSKTGEEVLGLFQQLNRQGNTVVLVTHDPHIASLAQRKVE
                     LRDGLVVAPEQKAA"
     gene            complement(3850..5070)
                     /locus_tag="PLB01_11455"
     CDS             complement(3850..5070)
                     /locus_tag="PLB01_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ77962.1"
                     /translation="MSKKLKWILSAAGIVVLAGGGVVFASRGKDKGKGPDQPPFRLGK
                     VQAEDLQVSVREVGVVDPETKVDVKSVVSGRVVSLKVRDGAVVHAGDVLAEVEPDVTQ
                     AQSLSDVRAGVTQAEVALRDAERQLASQERLFKEGLIGSESLKDFVTRRDLASENVKA
                     ARQRYQIVEGHGIPISGDAVTQRARVTAPMSGVVITKGVELGETVTSGVSSFNAGTVL
                     FTVADLKTLLIKVNLNEVDIAKVKVGQSVRITLDAYPQKNFSGRVRFVAPAAKLQDKI
                     KVFPVEIALDELGDAYRTGMSANVEILGEKKDKAVSIPLEALQRREGKTVVYRLKDNL
                     APKQIAAAKEGLGGRGKYIWLSDHWKDYYEVVPVTAGIATLERVEIVSGLKGTEQVAL
                     EDPTKKKIEKDDDN"
     gene            5155..6915
                     /gene="dauA"
                     /locus_tag="PLB01_11460"
     CDS             5155..6915
                     /gene="dauA"
                     /locus_tag="PLB01_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HQQ77963.1"
                     /translation="MALTRYLAGSEAPSLWRLPAAALRAVLREGYTAADFRRDALAGA
                     VVGTVALPLSMALSIAIGAPPQYGLYTAIVGGFVVALLGGSRTQVTGPTAAFVVILAP
                     IYARFGAAGLFMAGLLAGIMLIAFGLLRLGKLIEFIPHPVTTGFTAGIATVIATLQVK
                     DLLGLKLSSNPEHFFERIEAMWRARATAAWPELVVGLATLALLLGLPRFTKRIPAPLV
                     ALPFAAVLALALPHYVPGFSVSTIATRFTSVIGGVTVHGIPRMPPLPMLPWNAAVPDG
                     EPSGLTLAMLRAASRGAFAIAMLAAIESLLSAVVADGMARTKHDPDAELFALGVGNVV
                     TPFFGGIPATGAIARTATNIRSGGRSPVASMVHAATVLVAVLVLAPAMGYIPMASLAA
                     LLLLVAWNMSEARFFAHTVRVAPKSDVAILLTCFVLTVAIDMVVSVSVGVVLAALLFM
                     RRMAEVTETRVLEGGSHESTGPLPAGVVVYDIDGPLFFGAAQKAMAALGVVDKNARAV
                     VLRMDGVPALDSTGLVALESALDLLRAHRCMAVITGLRPQPEGALRKAEVEARHKVRL
                     ARDLPEALALIERVKGGARA"
     gene            6955..8364
                     /locus_tag="PLB01_11465"
     CDS             6955..8364
                     /locus_tag="PLB01_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HQQ77964.1"
                     /translation="MRFATLAAALLVSSSALAGPAAPGPEWFAAHRAALVAKLPKDAV
                     VVLRGPAEPPVEVGDAYRADSNFWYLTGFPEPDAIAVLRPSAPEGKRYLLFVRPNEWR
                     AEQWTGRRAGVDGAKKTYGADAAFPVADFEKESREALRGAKALFYLDAGDEKFRERLL
                     TGWNRMAHAGTEGLPATDVSVLAGQMRLVKDAAELALIREAVTLSVRGHRAAMALAAP
                     GTNEGVLKAAQVSACLAGGGVRMAYPPIVGSGPNSVIFHYPDANRTMQAGEIVVNDTA
                     CEYGMYAADVTRTYPVDGKFSPEQKALYEIVLAAQKAGIAKAVVGAAYHEIQDAAIAV
                     TVDGLVKLGLLKGTREELVKDRSFAKFLPHGTGHWLGLDVHDAGSYEPGTWPDPKDRF
                     AASMSFATKLKPGMVLTVEPGIYIPAKMEGVDAKWWNTGVRIEDDVLVTEKGPECLSC
                     DLPRELLDVERAVTVLRNR"
     gene            8376..>9987
                     /gene="mutL"
                     /locus_tag="PLB01_11470"
     CDS             8376..>9987
                     /gene="mutL"
                     /locus_tag="PLB01_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQQ77965.1"
                     /translation="MPGPVRVLPSALVDQIAAGEVVERPASAVKELVENALDAGARRV
                     TVEVEGGGVARISVLDDGSGMGPEDAALALERHATSKIRSFEDLTRVSTMGFRGEALP
                     SIASVSRLVLTTSPDASGLGTEVVADRGAAPTVRPARHPKGTRVVVEDLFGAVPARRK
                     FLKSAEGELRAVVRALTTLALANPSVAFSLRAGDRLLLDLPAAADAAARFREVLGDAL
                     PLPPLPVAFAHGGMSLSGAVTPPDVTFASRGNQWLFVNGRAIKDATASHAAALAAREV
                     LRSDRHTGFALFLACDPAACDVNVHPQKLEVRFREPNVVHALVHRGLVAALTGGKGSV
                     AVEGEAFGGREREERRGDFFERGREEGAAGWLGEGAARYGSGASGFGRYGADTRTLLD
                     AIAPGLSPSSYSEEISFSVSSSPLGTLRLLGQYRDSFLVAESEEGLVLVDQHVAHERV
                     RYERFLAKLEAASPASQALLTPLTFEAKPEEAALLGREDELLASAGFAVSELSGRTFL
                     VTAAPADMRASAVVAALRDFLDRLAALPD"
BASE COUNT         1399 a         3502 c         3521 g         1565 t
ORIGIN      
        1 aagcaactct tcgctcaggg cgccttgcct cagcacgacc gggtgtggct ccagtgcgag
       61 aaccgtggac tcgacgccgt gcgccgtcgg gccgccgtcg aggacgtaga cgtccgggaa
      121 atcctcggcg acgtgcgcgg ccgtcgtcgg gctcgggcgc ccggaaaggt tcgcgctcgg
      181 cgccgcgatc ggtacgcccg cgaggcggac gaggtcgcgc gcgaggccct ccggcatccg
      241 gaccgcgacg gtgtcgaggc cggcggtgac cgaagagggg attgaagagt tcttggaaag
      301 tatgagggtg aggggccccg gccaaaagcg ggctgccagt cgcgtggcga gcggcggcat
      361 ttgccgaatt acccttctaa gcatcttcaa atcgctcacg tgcacgatca ccgggttgtc
      421 gtgcgggcgg cctttcttgg cgaagatctt cgcgaccgcc ttcgcgtcca gcgcgttcgc
      481 cccgaggccg tagacggtct ccgtcgggaa cgcgacgagc tctcccttcc tgaggcgggt
      541 ggcggccgcg cgcagcgcgg ccggggtcgc gggatggacg ttcttggggc gcttcacgtc
      601 cgcatcttag cggcgcgcgg agcggggccg cgtcagcgca tcgcctccat gggcgagagg
      661 cgcgaggcgc ggatcgcggg gtagaggccg aagacgagag cgaggccgag cgcgaccgcc
      721 cacgccgtca cgaggcccat cgggttgacg acgagcccgt acgggaactt cgacgagagc
      781 gccttgcaca ggacggcgcc gccgatcgtt ccgagcaacg cgccgagcgc cgcgaggacg
      841 caggcctcga ggaggaactg cacgaagatc tcgccgtccg acgcgccgag cgacttgcgc
      901 agcccgatct cgtaccggcg gtccgagaac gagatgagca tgacggagag gacgccgacg
      961 ccgccgacga gaagcacggt cgccgcgagg ctcgagagaa cgacgcgcca gccgtacatc
     1021 tgctcgagga agcccgcgta ggacttcgcg gcctcggact cgaggtcctt gatctcgacg
     1081 tcctcgatcc cgtggtgggc ctgcttcgtc cggccgagga cgagcgcccg gacctcgtcg
     1141 aggtcggacc gcttgcgaag cttcacggcg acgctcgtga gcttgtggct cgcgtccatg
     1201 cggtccatgt acgtctcgag agggatcatg aggccgttcg agtcgatcca gagctcctcg
     1261 ttgaagatct ggaccggggc ctggaggccg acgatccgga acgggatccc gtccacgagg
     1321 acgtcgcggc cgacggggtc cgcgcctccg aagagcttga cgccgagcgt cgagccgacg
     1381 acggcgacgg cgctgcgccg cttgcggtcg tcctcggtga ggccgcggcc ggaggcgatc
     1441 ggccggttca tgaggagggc gtagtcgggg gtcaggcccg tgacccagat tcgctcgggg
     1501 ccgccggcgg tccggatccg cgtggacttc gtcgcgcgcg gcaggaacgc gaccgcggcc
     1561 ggatgcggcg ccgccacgcg ggagaggtcc tcgtaccgga ggcccgggct catcgcgaag
     1621 cgcttctgct cctccgtcgt ctcgggcgcc ttgggctgga tcgcgaccgt gccgtcccag
     1681 ctcatccccg agaagccgat cgagaccttg tccatgacgc cgtcgaggac ggaggtcatg
     1741 acgacgagcg cgaagacgcc gagcatgagg agcgtgagcg tgaggagcga gcgcaccttg
     1801 tgcgcccaca gctcgatgaa cccgccgatc acgacctccc agaggatgcc gatcttggcg
     1861 ccccacgaga gacgccgctc ggcgacgacc ggcggcgcca cggcaagggc cacgctactc
     1921 ataccgcagc gcttccatcg gggagaggcg gctcgccttc cacgcggggt agagggcgaa
     1981 gaggaagccg aacccggccg cgagcacgaa ggcccacagg aagctcgcgg gctgcatgta
     2041 gagcgggatc tggaggaacg acgtgatcgc cttcgagaag gcgatgccgc cgaggagccc
     2101 ggccacggcg ccgaagaccg tcaggacgag ggcctcggtc atgaagccct tgaagatctc
     2161 cgggcccgag gcgccgatcg ccattttcac gccgacctcg cggacgcgct ccttgaggct
     2221 cgccatctgg atgttcacgt tgaccatgcc gccgccgata agcgacagga tccccgagag
     2281 catgaagatg atgttgtaga cgtcgccctg gctccgccgc ttgcggatgc gcgcggcgat
     2341 gtcgtcgatc cggaagtcct cctgctggcg gtggttcccg aggaggaggc ccgtcaggcc
     2401 ttccgtgagc ttctcgccga cgttcaggtc gcgcatccgg aagacgacgc ggtcgacgcg
     2461 ctgccagcga tcgccgctca tgcgctgggc gacgagcgtg gcgggcaccg cgatgatccg
     2521 gttgcgccac cagaactggt ttccctggtt ctcgcgaaag cggaagacct tctccgcgaa
     2581 cgtgccgacg acgacgacgg ggatgtcccc gacacggatc gtctgcccga cggcgtcgcc
     2641 ggtgggaaag aacgtggaaa cggcctcggc gccgaggatc gcgacggcct ggcccttttc
     2701 gaagtcgtcg gcggcgaaac cgcgcccctt ctccaccgtg tagccgttca gcgcgaggta
     2761 gtcgccgccg atccccgtga tctggcggtc ctggtccgcg aacgccgagc ggacgcgcgc
     2821 gcgcgcgttc ttctgctgcg cgaccccgga cgtcgctttc tggtccagcg cgtcgccctc
     2881 gacggcgtcc tcgttgcgga ggccgaggtt cgccgtctgg agcgcggtcg gctggccgtc
     2941 cttgaccgcc tgcgacggct ggacgttcag ccggtccatg ccgccgagct tcacccagcg
     3001 ctcgtccgag cgcttccgct ggctgtccga gatcgagaac ccgccgagga ccgacgcgac
     3061 gccgaggacg acgccgaggg tctggagcgt cgagcggccg aggttttcgc ggatctcgac
     3121 ccaggccccg aagaagcgct cgcggaaggc gcccgagaac atcaggccgc cttctgctcg
     3181 ggtgcgacga cgaggccgtc gcgcagctcg accttgcgct gggcgaggga ggcgatgtgc
     3241 gggtcgtgcg tgacgaggac gacggtgttg ccctgccggt tcagctgctg gaagagtccg
     3301 aggacctcct cgcccgtctt cgagtcgagc gcccccgtcg gctcgtccgc gaggagaagc
     3361 gccggcgtgt tcgcgagggc gcgtgcgatc gcgacgcgct gtttctggcc gcccgagagc
     3421 tgcgcgggga ggacgttcgc cttctcgggg atcccgacct gctcgagcag ctcgagggcg
     3481 cggcggcggc ggtccttgcg cgacatgccc gcgtagagga gcgggagctc gacgttgcgg
     3541 acgatcgacg ccttcggaag gaggttgtag ctctggaaga cgaagccgat cttctcgttc
     3601 cggatgcgcg cgagggccgt gcccgagagg ccctgcaccg cgacgccgtc gagggcgtac
     3661 gtgccgtccg tgggcgagtc gaggcagccg atgatcgcca tgagcgtcga cttacccgat
     3721 cccgaggggc cgacgagggc gacgaactct ccgcgcgcga cggtcaggtc gatcccgcgg
     3781 agcgcgtgga cggccattcc gttcgacccg aagaccttcg tgaggccggc cgtctcgatg
     3841 atcgcccggt cagttgtcat cgtccttctc gatcttcttc ttcgtcgggt cctcgagggc
     3901 gacctgctcg gtgcccttga gacccgagac gatctcgaca cgctcgagcg tcgcgatgcc
     3961 ggccgtcacg gggacgacct cgtagtagtc cttccagtgg tccgagagcc agatgtactt
     4021 gccgcggccc ccgaggcctt ccttggcggc ggcgatctgc ttcggcgcga ggttgtcctt
     4081 caggcggtag acgacggtct tgccctcgcg cctctgcagg gcctcgaggg ggatcgagac
     4141 ggccttgtcc ttcttctcgc cgaggatctc gacgttcgcg ctcatgccgg tgcggtacgc
     4201 gtcgcccagc tcgtcgaggg cgatctcgac cgggaagacc ttgatcttgt cctggagctt
     4261 cgcggcggga gccacgaacc ggacccgtcc cgagaagttc ttctgcgggt aggcgtcgag
     4321 cgtgatccgg acggactggc cgaccttgac cttcgcgatg tcgacctcgt tgaggttcac
     4381 cttgatcagc agcgtcttga gatccgcgac cgtgaacagg accgtcccgg cgttgaacga
     4441 cgacacgccg gacgtgacgg tctccccgag ctcgacgccc ttcgtgatca cgacgcccga
     4501 catcggggcc gtgacgcggg cgcgctgcgt caccgcgtcg cccgagatcg ggattccgtg
     4561 accctcgacg atctggtagc gctgacgggc ggccttgacg ttctcggacg cgaggtcccg
     4621 cctcgtgacg aagtccttga gcgactcgct gccgatgagc ccttccttga agaggcgctc
     4681 ctgggaggcg agctggcgct ccgcgtccct gagggcgact tccgcctgcg tcacacccgc
     4741 ccggacgtcg gagagggact gggcctgggt cacgtccggc tcgacctcgg cgaggacgtc
     4801 gccggcgtgc acgacggctc cgtcgcggac cttgagggac acgacgcgac ccgagacgac
     4861 cgatttgacg tcgaccttcg tctcggggtc gacgacgccg acctcccgga cgctgacctg
     4921 gaggtcctcg gcctggacct tcccgagccg gaagggcggc tgatccggac ccttgccctt
     4981 gtccttgccg cgggatgcga aaacgacgcc ccctccggca aggaccacga ttccggcggc
     5041 cgagaggatc catttgagct tcttggacat gcgaaacctc gctcccgtat acgagagccc
     5101 gggccggaat gttctccgga atgagccctt tcgggctggt atcgtggggg gtcgatggcc
     5161 ctgacccgct acctggccgg ctccgaggcg ccgtccctct ggaggctgcc tgccgccgcc
     5221 ctccgtgccg tcctccggga ggggtacacc gccgccgatt ttcgccgcga cgccctcgcg
     5281 ggggccgtcg tcgggacggt cgctctgccg ctttcgatgg ccctctcgat cgccatcgga
     5341 gcgcccccgc agtacgggct ctacacggcg atcgtcgggg gattcgtcgt cgccctcctc
     5401 ggcggctccc gcacccaggt cacggggccg acggcggcgt tcgtcgtcat cctcgctccc
     5461 atctatgcgc gcttcggcgc cgcgggcctc ttcatggcag gccttctcgc cgggatcatg
     5521 ctgatcgcgt tcggcctcct ccggctcggc aagctcatcg agttcatccc ccaccccgtg
     5581 acgacgggat tcacggcggg gatcgcgacc gtgatcgcga cgctgcaggt caaggacctc
     5641 ctgggcctca agctctcctc caatcccgag catttcttcg agaggatcga ggcgatgtgg
     5701 cgggcgcgcg cgacggccgc gtggccggaa ctcgtcgtcg ggctcgcgac gctcgcgctc
     5761 ctcctcggcc ttccgcggtt cacgaaacgg atcccggcgc ccctcgtcgc cctcccgttc
     5821 gcggccgtcc tcgcgctcgc gctcccgcac tacgttccgg gcttctccgt ttccacgatc
     5881 gcgacgcggt tcacgtcggt catcggcggc gtcacggtgc acggcatacc gcggatgccg
     5941 ccgctgccca tgctcccgtg gaacgccgcc gtgccggacg gagagccctc cggcctcacg
     6001 ctcgcgatgc tccgggccgc ttcgcgggga gcgttcgcga tcgcgatgct cgcggcgatc
     6061 gagtcgctcc tctcggccgt cgtcgcggac ggaatggcgc gcacgaagca cgacccggat
     6121 gccgagctct tcgcgctggg ggtcggaaac gtcgtgacgc cgttcttcgg gggcatcccc
     6181 gcaacgggcg cgatcgcgcg cacggccacg aacatccgtt cgggcggccg gtcgcccgtc
     6241 gcttcgatgg tgcacgcggc cacggttctc gtcgcggtgc tcgtcctcgc acccgccatg
     6301 gggtacatcc cgatggcctc gctcgccgcg ctgctcctcc tcgtggcctg gaacatgtcg
     6361 gaggcccggt tcttcgccca caccgtgcgc gtcgcgccga agagcgacgt cgcgatcctc
     6421 ctgacctgct tcgtcctgac ggtggccatc gacatggtcg tgtccgtgtc cgtgggcgtc
     6481 gtcctcgccg cgctgctctt catgcgccgc atggccgagg tgaccgagac gcgcgtcctc
     6541 gagggcggct cccacgagtc gacaggcccg cttccggcgg gcgtcgtcgt ctacgacatc
     6601 gacgggccgc tgttcttcgg ggccgcgcag aaggccatgg ccgcgctcgg ggtcgtcgac
     6661 aagaacgcgc gggcggtcgt gctgcgcatg gacggcgtcc ccgcgctcga ttcgacgggc
     6721 ctcgtcgcgc tcgagagcgc gctcgacctc ctccgggcgc accgctgcat ggccgtcatc
     6781 acgggcctgc gcccccagcc cgagggcgcg ctcaggaagg ccgaggtcga ggcgcggcac
     6841 aaggtccgcc ttgcacgcga cctgcccgag gcgctcgccc tgatcgagcg cgtcaagggc
     6901 ggcgcccgcg cctgagacgg attcgcggct aaactccgcg ctccggagga acccatgcgc
     6961 ttcgcgacgc tcgccgcggc cctgcttgtt tcatcgtccg cccttgccgg ccccgccgcg
     7021 cccggccccg aatggttcgc ggcccaccgg gccgccctcg tcgcgaagct gcccaaggac
     7081 gccgtcgtcg tcctgcgggg cccggccgag ccgcccgtgg aagtcgggga cgcctaccgg
     7141 gccgactcga acttctggta cctcacgggg ttccccgagc ccgacgcgat cgcggtcctg
     7201 cgcccgtcgg ctcccgaggg gaaacgctac ctcctcttcg tgcgcccgaa cgagtggcgg
     7261 gccgagcagt ggaccggccg ccgcgcgggg gtcgacggcg cgaagaagac gtacggcgcg
     7321 gacgccgcgt tcccggtcgc ggacttcgag aaggaatcgc gcgaggcgct gcggggggcg
     7381 aaggcgctct tctacctcga cgcgggcgac gagaagttcc gcgagaggct cctgacgggc
     7441 tggaaccgga tggcccacgc cgggacggag ggccttcccg cgaccgacgt ctccgtactc
     7501 gcgggtcaga tgcgcctcgt gaaagacgcg gcggagctcg ctctcatccg ggaagccgtc
     7561 acgttgtccg tgcgcggcca ccgcgccgcg atggcgctcg cggccccggg aacgaacgag
     7621 ggcgtcctga aggcggcaca ggtctccgcg tgcctcgcgg gcggcggcgt gcgcatggcg
     7681 tacccgccga tcgtcggctc cgggccgaac tccgtcatct tccactatcc ggacgcgaac
     7741 cggacgatgc aggcgggcga gatcgtcgtg aacgacacgg cctgcgagta cgggatgtat
     7801 gcggccgacg tgacgcggac ctaccccgtg gacgggaagt tctctccgga gcagaaggcg
     7861 ctctacgaga tcgtcctcgc ggcgcagaag gccgggatcg cgaaagcggt cgtgggcgcg
     7921 gcgtaccacg agatccagga cgcggcgatc gccgtcacgg tcgacggcct cgtgaagctc
     7981 gggctcctca aggggacgcg cgaggagctc gtgaaggacc gctcgttcgc gaagttcctc
     8041 ccgcacggaa ccggccactg gctcggcctc gacgtccacg acgcggggag ctacgagccc
     8101 ggcacgtggc ccgacccaaa ggaccgtttc gccgccagca tgtccttcgc gacgaagctg
     8161 aagcccggga tggtcctcac ggtggagccc gggatctaca tccccgcgaa gatggaaggc
     8221 gtcgacgcga agtggtggaa cacgggcgtg aggatcgagg acgacgtcct cgtgaccgaa
     8281 aagggccccg agtgcctctc gtgcgacctg ccgcgggagc tcctcgacgt ggagagggcc
     8341 gtcacggtcc tgaggaaccg ctaacatccc ggcccgtgcc gggtcccgtt cgcgtccttc
     8401 cctccgccct cgtcgaccag atcgccgcgg gcgaggtcgt cgagcggccc gcgtccgcgg
     8461 tcaaggagct cgtcgagaac gcgctcgacg cgggtgcgcg gcgcgtcacc gtcgaggtcg
     8521 agggcggcgg cgtcgcgcgg atctcggtcc tcgacgacgg gtcggggatg ggccccgagg
     8581 acgcggccct cgcgctcgaa cgccacgcga cgtcgaagat ccgttccttc gaggacctca
     8641 cgcgcgtttc gacgatgggg ttccggggcg aggcgctgcc gtcgatcgcc tcggtctcgc
     8701 gcctcgtcct cacgacgtcc cccgacgcct cgggcctcgg gaccgaggtc gtcgcggacc
     8761 gcggcgcggc cccgaccgtc cgccccgcgc gccacccgaa gggcacgcgg gtcgtcgtgg
     8821 aggacctctt cggggcggtt cccgcgcgcc ggaagttcct caagagcgcc gagggcgagc
     8881 tgagggccgt cgtccgggcg ctcacgacgc tcgcgctcgc gaacccctcc gtcgccttct
     8941 cgcttcgcgc cggggaccgc ctgctcctcg accttcccgc cgccgcggac gccgccgcgc
     9001 gcttccgcga ggtgctcggt gacgcgctcc cgctcccgcc gcttccggtc gcgttcgcgc
     9061 acggcggcat gtcgctctcg ggcgccgtga cgccgccgga cgtgacgttc gcctcgcgcg
     9121 ggaaccagtg gctcttcgtg aacggccgcg cgatcaagga cgcgaccgcc tcccacgcgg
     9181 ccgccctcgc cgcgcgcgag gtcctgcgct cggatcgcca cacgggcttc gcgctcttcc
     9241 tggcgtgcga ccccgccgcc tgcgacgtga acgtccaccc ccagaagctc gaggtgcgct
     9301 tccgcgagcc gaacgtcgtc cacgcgctcg tccaccgcgg cctcgtggcg gcgctgacgg
     9361 gagggaaggg ttctgttgcc gttgaagggg aagcgttcgg aggaagagag agagaagaga
     9421 gaagagggga tttctttgaa aggggaagag aggaaggggc ggccggctgg ctcggcgagg
     9481 gggcggcgcg ctacggctcg ggcgcctcgg gcttcgggcg ctacggcgcc gacacgcgga
     9541 cgctgctcga cgcgatcgca ccgggcctat ccccctcttc ctattcagaa gaaatctctt
     9601 tttcggtctc ttcgtctcct ctcggcacgc ttcgcctttt gggccagtac cgcgactcct
     9661 tcctcgtcgc cgagagcgag gaggggttgg tcctcgtgga tcagcacgtc gcgcacgagc
     9721 gcgtgcgcta cgagcgcttt ctcgcgaagc tcgaggcggc gtctcccgct tcccaggcgt
     9781 tgctgactcc gctcacgttc gaggcgaagc cggaggaagc ggcgctcctc ggacgcgagg
     9841 acgaactcct cgcctcggcc ggcttcgcgg tctcggagct gtcgggccgg acgttcctcg
     9901 tgacggccgc gcccgcggac atgcgcgcct cggccgtcgt tgccgccctg cgcgacttcc
     9961 tcgaccgcct cgcggcgctg ccggacg
//
LOCUS       DAOMFY010000133         9886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2085_length_9886_cov_7.274438, whole genome shotgun sequence.
ACCESSION   DAOMFY010000133 DAOMFY010000000
VERSION     DAOMFY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_9886_cov_7.274438"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1071)
                     /locus_tag="PLB01_11475"
     CDS             complement(157..1071)
                     /locus_tag="PLB01_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77966.1"
                     /translation="MARLQGPPARVTGRRVAVALGAVFLAAAAALTFGAARWRLRTRD
                     FDASLRAAKSTGAPRTFDARELEHLPAPVRRYLAIALRPGQPVIESARVATSGEFLLD
                     AEKKRWAPFTASQLVTVDPPGFDWDARIRMLPGLDVFVRDAYREGAGVLRAEGAGLVT
                     VADLPASPDLARGELLRWLAEAPWFPTALLPSQGVRWEALAEDRATATVVDRGVTARV
                     EFRFGPDGLPASIFVPDRPRTVGAAIVPTPWEGTWSAYGRRNGMRVPSAGEVAWLLPE
                     GRLPYWRGRVRSAVYTFSNADAAPVVPR"
     gene            complement(1038..1505)
                     /locus_tag="PLB01_11480"
     CDS             complement(1038..1505)
                     /locus_tag="PLB01_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQQ77967.1"
                     /translation="MARVPESVASFLQGKRFAVAGVSRDGKLPANAIFRKLKASGYDV
                     VPVNPNAETLEGVPSYPNLAAIPGPLDGLVIAAHPDVAAGLVKQAAARGVKQVWFHRS
                     FGEGSVSGAALDACRAAGIDPIVGGCPLMYCAPVDVAHRCFRWWLGFKGRLPA"
     gene            complement(1518..1973)
                     /locus_tag="PLB01_11485"
     CDS             complement(1518..1973)
                     /locus_tag="PLB01_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77968.1"
                     /translation="MELSRLLEETRAEAVADAIQGLSRATLPHYTASRSEQNRERLAK
                     LYDLTTACVATRTLLPMIEYAQGVARERYRDGFDLEEVHTAFNVLEEVIWRRITSRME
                     PPQYPEAFGLVSTVLGAGKQALAIEYVALVSQNRDVHSLDLTALFKGTT"
     gene            2091..3926
                     /locus_tag="PLB01_11490"
     CDS             2091..3926
                     /locus_tag="PLB01_11490"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ77969.1"
                     /translation="MKTFRAAPVALLALSARLLAQPAPAPALSPAAARVKADVAFLAS
                     DDLKGRRAGTAEGDRAAAWVAEQFRKIGLAPAGPNGAWLQPFDFIDGVDLGPKNRLET
                     AAGAKKAWTAGNDFRPLAFSAAGLAEGDVVFAGYGIVSKDLGYDDYAGLDVKDKIVLV
                     LRYSPDGDDEKSAFSPFAALRFKAAVAREKGAKAMLVAAGPLTKDVADDLIALRTDAA
                     FSDAGIVAVSVRRPVAEALLAGSGKTLEAAQKAIDDAKKPASFAVPGSRATANVDVTP
                     RRVRAANVVGVLKGADSVKSAEFVIVGAHWDHLGLGGTTSLASASGPQIHHGADDNAS
                     GVAALLETARGLAAKRATLPRSILFISFAAEELGTLGSLHFTKNPTVPWDSVVSMFNL
                     DMVGRLRENKLDVQGVGTSPAWKGFVEASNKDANLKLALLDGGFGPSDHSPFYAAKKP
                     VLFVFTGAHGDYHKPSDTADRIDAEGIVRVVNFVEPVIVAAASAPERIAYAEVKGGAP
                     AGGGSRSFRVWVGGIPDFSEEGKGVKLSGVTGGSPAEKAGLAAGDTIVKFGDKELRNL
                     YDYTYALQGRKPGEKVNLVVLRVEDGKAVEKTFEVTLGSRPDATK"
     gene            complement(3946..6591)
                     /locus_tag="PLB01_11495"
     CDS             complement(3946..6591)
                     /locus_tag="PLB01_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ77970.1"
                     /translation="MSLSPGTRLGPYEILSQVGAGGMGEVYRARDSRLQREVAIKVLP
                     PAVAADADRLSRFEREARSASALNHPNIVTIHEIGEAGAVRFLVMELVSGSTLQELLS
                     GGPLPIRRLLQIAAQVAEGLARAHSAGIVHRDLKPANLMLTSDGVAKILDFGLAKLEP
                     PRTDAEVLSQLDTEPGRTEPGVIVGTVGYMSPEQASGAPVDFRSDQFSFGTILHEMAT
                     GQRAFLRPTAVETLAAIVREEPRPLGSVRPDAPAPLRWVVERCLSKDPRERYASTEDL
                     ARDLATLRDRLSETAAPAGAGPPSSRRRALAAGWALALLFLALLVVSSLRRRESPALP
                     AGIDATITQLTNYGESERSGAISPDGRQFAFVSEKGGSPDIWVRQVSGGEPRQVTRDD
                     AEETDLVYAPDGESLYYVAWGPDRPSIWRIPVLGGTPRKIVETARYPAPAADGKRLAW
                     VRTFVPDFPLWVDRGSIEVGNADGSGARKVYEGLGIGAVSWSPDGRRLAFTAAPFFET
                     RNLHVLDVESAREAQITRFPSGSVYSQAWLPGNRNLVFSRVSSGYVLASTADLGIVSY
                     DGTVQRRLTLAVNARFVGPRVAADGKRLLATMETDQHEVWRVPLGPDPEANGRDAVRL
                     LDGSGDPFWTQVSKDGSTLLFNSSAAGSRNLWTMSLDGAASPRQITFMNSNALTHSAL
                     SPDGTRVAYASHQTGISKIWVASVDGSNGVQLTDGLAQDFWPTWSPDAKWIAFGSLRS
                     GDPQLWKVPSTGGKAEMVTREGGFRGEWSPVDNRIVYWKAGQIQVFDADSGKVLLTVP
                     AGDLMWTLPAWSPDGRSFSAIREERRGNSSLWIFDARTGARRLAALLPTGFHTYFRAS
                     WGRGGQSLIVNRGRTASRVVLLENF"
     gene            complement(6639..6926)
                     /locus_tag="PLB01_11500"
     CDS             complement(6639..6926)
                     /locus_tag="PLB01_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HQQ77971.1"
                     /translation="MPAYILVEIDVHDAAGYARYRETVMPSITAYGGRFLVRGGAIET
                     LEGDWHPPRLAMLEFPDREKARSWWSSPEYAGPKAMRLATARSRMILVDGV"
     gene            complement(6941..7288)
                     /locus_tag="PLB01_11505"
     CDS             complement(6941..7288)
                     /locus_tag="PLB01_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77972.1"
                     /translation="MSEPVVELWGFPDPDIEAVRAVVERTLGVTMELHESASIGPYYH
                     ASLCGAHADLVLRPNLDASFDQETDDPDECLEEPDFPDFGSLLYVEWATPGHTCRQSV
                     DALGAEAQMLIVE"
     gene            complement(7362..7922)
                     /locus_tag="PLB01_11510"
     CDS             complement(7362..7922)
                     /locus_tag="PLB01_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQQ77973.1"
                     /translation="MRPLRYSVNVTLDGCCDHRAIIPDEALHRHAAENLARADALLFG
                     RVTYEMMEAAWRAPARTGERPEWMQPWMEPFARTIDAAKKYVVSSTLNRVDWNAELVR
                     GDLGKAVQRLKSESGHGLFVGGVKLALALTELGLIDEYEFVVHPRLAGHGPTLFAGLS
                     KHVDLKLVSRLELGSGAVAMRYEAKR"
     gene            complement(7922..8428)
                     /locus_tag="PLB01_11515"
     CDS             complement(7922..8428)
                     /locus_tag="PLB01_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQQ77974.1"
                     /translation="MKERPVLTVNQWLASVPAERKDAINAVRKAVNKHLPRGYEETVD
                     WGMLAWVVPLATLPNTHNGRPLLLAALGAHTKRMTLYLMSVYGDPKLRREFETAYKRT
                     GKKLDMGGCCVHFKTLDDLPLDVVGNTVARVTVDQYVERYESLRKKKSPKAKAKAKTK
                     AKAKAKRA"
     gene            complement(8546..9217)
                     /locus_tag="PLB01_11520"
     CDS             complement(8546..9217)
                     /locus_tag="PLB01_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQQ77975.1"
                     /translation="MTRVRVEGFTISLDGYGAGPNQDIRNPLGVGGTGLHQWLVPTRT
                     FQRTLFGADGGTTGIDDDFAARGFRNVGAWILGRNMFGPVRGPWPDMNWKGWWGDNPP
                     YHVRTFVLTHHARPPIPMEGGTTFHFVTGGIHEALDRAREAANGMDVRIGGGPATIQQ
                     YLRAGLVDELHVAISPVLLGGGERLFEGIDARALGYECTQFVASDKAAHVVLRRQGHD
                     DARKR"
BASE COUNT         1537 a         3423 c         3454 g         1472 t
ORIGIN      
        1 tggccatgga gaagagcacg gggggagcgt tccgggggac cgggccggcg gccgtgagcg
       61 tcccgacggt ggcgccgacg acgctggagt gcgtgggcgt gcccccggtc gagggcaggg
      121 tcattccggc gcgccgcgcc tcgaaggcgt agcctttcag cgcggaacga cgggggcggc
      181 gtccgcgttc gagaacgtgt agacggccga ccgcacgcgg cctcgccagt acggaagccg
      241 cccctcggga aggagccagg ccacctcgcc ggccgacggc acccgcatcc cgtttcggcg
      301 accgtaggcc gaccacgtcc cctcccacgg cgtcggcacg atcgccgcgc cgaccgtccg
      361 gggacggtcg gggacgaaga tcgacgcggg aaggccgtcg ggaccgaacc ggaactccac
      421 gcgggcggtc acgccgcggt cgaccacggt cgccgttgcg cggtcctcgg cgagggcctc
      481 ccagcggacg ccctggctgg gaaggagcgc cgtcgggaac cacggagcct cggccagcca
      541 gcgcagcagc tcgccgcgcg cgagatccgg cgacgcggga aggtccgcga ccgtcacgag
      601 gccggcgccc tcggccctca ggacgcccgc gccttcgcgg tatgcgtcgc gcacgaagac
      661 gtcgaggccc ggcagcatcc ggatgcgcgc gtcccagtcg aagccgggcg gatccaccgt
      721 gacgagctgg ctcgcggtga acggcgccca gcgtttcttc tccgcgtcga ggaggaactc
      781 gcccgaggtc gcgacgcgtg ccgactcgat cacgggctgg cccgggcgca gggcgatcgc
      841 gaggtaccgg cggacgggcg cggggaggtg ctcgagctcg cgcgcgtcga acgttcgcgg
      901 cgctcccgtg gacttcgcgg cccggaggga cgcgtcgaaa tcgcgcgtcc gaaggcgcca
      961 acgcgccgct ccgaacgtca gcgcggcggc ggcggcgagg aagacggccc cgagggcgac
     1021 ggcgacccgg cgccccgtca cgcgggcagg cggcccttga agccgagcca ccagcggaag
     1081 cagcggtgcg cgacgtccac cggggcgcag tacatgagcg ggcagccgcc cacgatcgga
     1141 tcgatcccgg ccgcgcggca ggcgtcgagc gctgcacccg agacgctgcc ctcgccgaac
     1201 gagcggtgga accagacctg tttcacgccg cgcgcggcgg cctgcttcac gaggccggcc
     1261 gcgacgtccg ggtgtgccgc gatcacgagc ccgtccagcg gtccgggaat cgcggcgagg
     1321 ttcgggtacg agggcacgcc ctcgagggtc tcggcgttcg ggttcacggg gacgacgtcg
     1381 tagccggacg ccttcagctt ccggaagatc gcgttcgcgg ggagcttccc gtcgcgcgag
     1441 acgccggcga cggcgaagcg ctttccctgc aggaacgagg cgacggactc ggggacgcgc
     1501 gccacgagcg ggactcccta ggtcgtgccc ttgaagagcg cggtgaggtc cagcgagtgg
     1561 acgtcgcggt tctggctcac gagggcgacg tactcgatcg cgagcgcctg cttgcccgcg
     1621 ccgagcaccg tgctcacgag cccgaacgcc tctgggtact gcggcggctc catccgggac
     1681 gtgatccgcc gccagatgac ctcctcgagc acgttgaacg ccgtgtggac ctcctcgagg
     1741 tcgaacccgt ccctgtagcg ctcgcgggcc acgccctgcg cgtactcgat catcgggagc
     1801 agcgtgcgcg tcgccacgca cgccgtcgtc aggtcgtaca gcttggcgag gcgctcgcgg
     1861 ttctgctcgg atcggctggc ggtgtagtgg gggagcgtgg cgcgtgagag gccctggatc
     1921 gcgtccgcga cggcctccgc gcgcgtctct tcgagcaacc gggaaagttc catcgactct
     1981 tccttcctcg agacgtccat cacctcaaag atagggccgc tcccacggtc cgcgccgtga
     2041 gtgcggttgc tctatcgcgg gagcgggagt tccgtggtaa agatccccgg atgaagacgt
     2101 tccgcgccgc tcctgtcgcg ctcctcgccc tttcggcccg ccttctcgcg cagcccgctc
     2161 cggcgccggc cctctcgccg gccgcggccc gcgtgaaggc cgacgtcgcg ttcctcgcct
     2221 ccgacgacct caagggccgc cgcgccggga ccgccgaagg cgaccgcgcc gccgcatggg
     2281 tggccgagca gttcaggaag atcggcctgg cgcccgcggg cccgaacggc gcgtggctcc
     2341 agcccttcga cttcatcgac ggcgtggacc tcggcccgaa gaaccgcctc gagacggccg
     2401 ccggcgcgaa gaaggcctgg accgccggca acgacttccg cccgctcgcg ttctccgccg
     2461 cgggcctcgc cgagggcgac gtcgtgttcg cgggttacgg catcgtctcc aaggacctcg
     2521 ggtacgacga ctacgccggg ctcgacgtca aggacaagat cgtcctcgtc ctgcgctaca
     2581 gcccggacgg cgacgacgag aagtccgcgt tctcgccgtt cgccgcgctc cgcttcaagg
     2641 cggccgtcgc gcgcgaaaaa ggcgcgaagg ccatgctcgt ggccgccggc ccgctcacga
     2701 aggacgtggc cgacgacctc atcgcgctgc ggaccgacgc ggcgttctcg gacgccggca
     2761 tcgtcgccgt ctccgtccgc cggcccgtcg ccgaggcgct cctcgcgggc tcgggcaaga
     2821 cgctcgaggc cgcgcagaag gccatcgacg acgcgaaaaa gccggcgtcg ttcgcggttc
     2881 ccggctcgcg cgccaccgcg aacgtcgacg tcacgccgcg ccgcgtccgg gcggcgaacg
     2941 tcgtcggcgt cctcaagggg gccgactccg tgaagagcgc cgagttcgtg atcgtcggcg
     3001 cgcactggga ccacctcggg ctcggcggaa cgacgtcgct cgcctcggcg agcgggccgc
     3061 agatccacca cggcgccgac gacaacgcgt cgggcgtcgc cgcgctgctc gagacggcgc
     3121 ggggcctcgc cgcgaagcgc gccacgctgc cgcggtcgat cctcttcatc tccttcgcgg
     3181 cggaggagct cggcacgctg ggttccctgc acttcacgaa gaacccgacc gtcccgtggg
     3241 actcggtcgt ctcgatgttc aacctcgaca tggtcggccg cctgagggag aacaagctcg
     3301 acgtgcaggg cgtcggcacg tctcccgcgt ggaagggttt cgtcgaagcg tccaacaagg
     3361 acgcgaatct gaagctcgca ctcctcgacg gcggcttcgg gccatcggac cactcgccgt
     3421 tctacgccgc gaagaagccc gtcctcttcg tgttcaccgg cgcgcacggc gactaccaca
     3481 agccctccga caccgccgac cggatcgacg ccgagggcat cgtccgcgtc gtgaacttcg
     3541 tcgaacccgt catcgtggcg gccgcctcgg cgccggagcg catcgcctac gccgaggtga
     3601 agggcggggc gcccgccggc ggcggatcgc gcagcttccg cgtgtgggtc ggcggcattc
     3661 cggacttctc cgaggagggc aagggcgtga agctctccgg agtcacgggc ggctcgcccg
     3721 cggagaaggc cggcctcgcg gccggcgaca cgatcgtgaa gttcggcgac aaggagctcc
     3781 gcaacctcta cgactacacc tatgcgctgc agggccggaa gcccggcgag aaggtgaacc
     3841 tcgtcgtcct gcgcgtcgag gacggcaagg ccgtcgagaa gaccttcgag gtcacgctcg
     3901 gctcgcgccc ggacgcgacg aaataggccg cgtcccgcgg cccgatcaga agttttccag
     3961 gaggacgacg cgtgaagccg tccttccgcg gttgacgatc aggctctgcc cccctcggcc
     4021 ccagctcgcg cgaaagtacg tgtggaatcc cgtcggaagg agggcggcca gtcgccgcgc
     4081 gcccgtccgg gcgtcgaaaa tccagagcga actgttcccg cggcgttctt cccgaatcgc
     4141 cgagaaggag cggccgtccg ggctccacgc cggcagcgtc cacatcaggt cgccggcggg
     4201 gaccgtgagc aggaccttgc cgctgtcggc gtcgaagacc tggatctgac cggccttcca
     4261 gtagacgatc cggttgtcga cgggagacca ctctcctcga aatcctccct cgcgcgttac
     4321 catctcggct tttcccccgg tgctcggcac cttccagagc tgtggatctc cgctgcggag
     4381 cgagccgaac gcgatccatt tggcgtcggg tgaccaggtc ggccagaagt cctgcgcgag
     4441 gccgtccgtc agctgcactc cgttcgatcc gtcgacgctg gccacccaga tcttcgagat
     4501 tccggtctga tgggacgcgt aggcaacgcg ggtgccatcg ggcgagagag cggagtgtgt
     4561 cagggcgttg ctgttcatga acgtgatctg gcggggggag gcggcgccgt cgagggacat
     4621 cgtccagaga ttgcggctgc ccgcggccga gctgttgaag agaagcgtcg agccgtcctt
     4681 cgagacctgg gtccagaagg gatctccgga gccgtcgagc agacggacgg cgtcgcgccc
     4741 gttggcttcc gggtctggcc cgagcggaac cctccagact tcgtgctgat cggtctccat
     4801 cgtcgcgagc aggcgcttgc cgtccgccgc gacgcgcgga ccaacgaagc gcgcgttgac
     4861 ggcgagcgtc aatcggcgct ggaccgtccc gtcgtaagag acgattccga ggtcggccgt
     4921 cgaggccagt acgtagccgg acgacacccg ggaaaagacg aggttcctgt tgccgggcag
     4981 ccaggcctgg gagtacacgg agcccgacgg gaagcgcgtg atctgagcct cgcgcgcgct
     5041 ctcgacgtcc aggacgtgga gattccgcgt ttcgaagaac ggggccgccg tgaaggcgag
     5101 cctgcgcccg tccggcgacc acgagaccgc cccgatgccg aggccttcgt acaccttccg
     5161 cgcgcccgat ccgtccgcgt tgccgacttc gatggagccg cggtccaccc agaggggaaa
     5221 gtccggaacg aacgtgcgca cccaggcgag ccgctttccg tccgccgcgg gcgcgggata
     5281 gcgcgccgtt tccacgatct tgcgcggcgt cccgcccagg accggaatgc gccagatcga
     5341 cggccggtcg ggtccccatg ccacgtaata gagcgactcc ccgtccggcg cgtacacgag
     5401 gtccgtctct tccgcgtcgt cccgcgtgac ttgccggggc tctcctcccg agacctgacg
     5461 gacccagatg tcgggcgagc cccccttttc ggagacgaag gcgaactgcc ggccgtcggg
     5521 cgagattgcg ccggaccgtt cactctcacc gtagttcgtc agctgggtga tcgtcgcgtc
     5581 gatgccggcg ggaagggccg ggctttcccg gcggcggagg gatgagacga ccaggagggc
     5641 gagaaagaga agagcgagag cccagccggc ggccagcgcc cgccgacggg atgaaggagg
     5701 accggcgccg gctggcgcgg ccgtctccga caagcggtcg cgcagcgtgg cgagatcgcg
     5761 cgcgaggtcc tccgtcgagg cgtaccgctc gcgcgggtct ttcgagaggc agcgctcgac
     5821 gacccatcga agaggcgcgg gcgcgtccgg gcgcaccgag ccgagcggcc ggggctcctc
     5881 gcgaacgatc gcggccaacg tctcgaccgc cgtgggacgc aggaaagcgc gctgacccgt
     5941 ggccatctcg tggaggatcg ttccgaagga gaactgatcc gagcggaagt cgaccggcgc
     6001 tccgctcgcc tgctccggag acatgtaccc gacggtcccg acgatgactc cgggttcggt
     6061 ccggccgggc tccgtatcga gctgcgagag cacctcggcg tccgtacggg gaggctcgag
     6121 cttggcgagg ccgaagtcga ggatcttggc gacgccgtcg gaagtcagca tcaggttcgc
     6181 gggcttgaga tcgcggtgga cgattcccgc ggaatgcgcg cgcgccagcc cctccgcgac
     6241 ctgagccgca atctgcagca ggcggcggat cggcaggggt ccgcccgaca ggagctcctg
     6301 cagcgtcgag ccggaaacga gctccatcac gaggaatcga acggcgcccg cctccccgat
     6361 ctcgtgaatg gtgacgatgt tcgggtgatt gagggccgag gcggaccgag cctctctctc
     6421 gaagcgcgaa agcctgtccg cgtcggcagc gacggccggg ggcaggacct tgatggccac
     6481 ctcgcgctgc aggcgcgagt cacgcgcgcg gtagacctcg cccatgccgc ccgcgccgac
     6541 ctgcgagagg atctcgtacg ggccgagacg ggttccggga gagagactca tccgcgtgca
     6601 gaggattcta tgcgtgagac gcgtgggatc gaggcgcgtc agaccccgtc tacgaggatc
     6661 atccgcgagc gcgccgtcgc gaggcgcatc gccttgggcc ccgcgtactc cggcgacgac
     6721 caccacgagc gggccttttc gcggtccggg aactcgagca tggcgagccg gggcgggtgc
     6781 cagtcaccct cgagcgtctc gatcgcgccg ccccgcacga ggaatcggcc cccgtaggcg
     6841 gtgatggacg gcatcacggt ctcgcggtaa cgcgcgtagc ccgcggcgtc gtggacgtcg
     6901 atctcgacca ggatgtaggc gggcatggga cctccgatcg ctattcgacg atgagcatct
     6961 gcgcttcggc gccgagcgcg tcgacggact ggcggcaggt gtggccgggt gtcgcccatt
     7021 cgacgtagag caggctcccg aagtcgggga agtcgggctc ctcgagacat tcgtccgggt
     7081 cgtccgtctc ctggtcgaag gacgcgtcga ggttcggcct caggacgagg tcggcgtgcg
     7141 cgccgcacag cgacgcgtgg tagtacggcc cgatcgacgc gctctcgtgg agctccatcg
     7201 tcacgcccag cgtacgttcc acgacggcgc gcacggcctc gatgtccggg tcggggaagc
     7261 cccagagctc gacgacgggt tccgacatga tcggattgtg cctgagggcc ggagcccggg
     7321 cgggccttct tgagacgccc gagggcaagt cgccggcggc gctacctttt cgcctcgtac
     7381 cgcatcgcca ccgcccccga gccgagctcc agccggctca cgagcttcag gtcgacgtgc
     7441 ttcgacagcc ccgcgaacaa cgtcggccca tggcccgcga gcctggggtg cacgacgaac
     7501 tcgtactcgt cgatcaaccc cagctccgtc aacgcgagcg cgagcttcac gcctcccacg
     7561 aagagtccgt ggcccgactc gctcttcagc cgctgaacgg ccttcccaag gtccccgcgc
     7621 acgagctccg cgttccaatc gacccggttc agggtgctcg acacgacata cttcttcgcc
     7681 gcgtcgatcg tccgtgcgaa gggttccatc cagggttgca tccactcggg ccgctctccc
     7741 gtccgcgccg gcgcccgcca cgccgcctcc atcatttcgt aggtcacccg gccgaacaga
     7801 agggcgtcgg cccgggcgag gttctcggcc gcgtgacgat gcagggcttc gtccgggatg
     7861 attgcgcgat gatcgcagca cccgtccagc gtgacgttga cggaatatcg aaggggacgc
     7921 atcaggctct cttcgccttc gccttcgcct tcgttttcgc ctttgccttc gccttcgggc
     7981 tcttcttctt gcgcaagctc tcgtagcgct cgacgtactg gtcgacggtg acgcgggcga
     8041 cggtattgcc gacgacgtcg agcggcagat cgtcgagcgt cttgaagtgg acgcagcagc
     8101 cgcccatgtc gagcttcttg cccgtcctct tgtaggcggt ctcgaactct ctcctgagct
     8161 tcggatcgcc gtagacgctc atgaggtaga gggtcatgcg cttcgtgtgc gcgccgagcg
     8221 ccgcgagcag cagcggacgg ccgttgtgcg tgttcgggag cgtggcgagc ggcacgaccc
     8281 aggcgagcat gccccagtcg accgtctcct cgtagccccg gggaaggtgc ttgttgaccg
     8341 ccttgcggac ggcgttgatg gcgtccttgc gctcggcggg caccgacgca agccactgat
     8401 tcacggtgag cacgggacgt tctttcacga ttcgctcctt ttcggctcat accgcgtcgg
     8461 cgcggcaccc gagacgggtt ccagccggcc gacgagcctc aggtcgtggg gcccccggag
     8521 catttccgcg gagcccacgg atgcttcagc gcttccgcgc atcatcgtgc ccttggcgcc
     8581 tgaggacgac atgggccgcc ttgtccgacg cgacgaactg agtacattcg taccccaggg
     8641 cgcgcgcgtc aatcccctcg aacagccgct cgcccccgcc cagcaggacc ggcgagatcg
     8701 cgacgtgcag ctcatcgacg aggcccgcac gaagatactg ctggatcgtg gcgggtccgc
     8761 cgccgatgcg cacatccatc ccgttggctg cttcgcgcgc gcggtcgagc gcctcgtgaa
     8821 tgccacccgt gacgaagtgg aacgtggtgc cgccttccat cgggatggga gggcgcgcgt
     8881 gatgagtcaa gacgaacgtc cgaacgtgat agggcgggtt gtccccccac cagcctttcc
     8941 agttcatgtc gggccagggc ccgcggaccg gtccgaacat gtttcttccg agaatccagg
     9001 cgccgacatt ccggaagccc cgcgcggcga agtcgtcatc gatccccgtg gtgccgccgt
     9061 cggcgccgaa cagggtccgt tggaacgtgc gggtcggcac cagccactga tgcaaacccg
     9121 tcccgcccac gccaaggggg ttcctgatgt cctgattcgg acccgctccg tacccgtcaa
     9181 gcgagatagt gaagccctcg actcggacgc gtgtcatgtc ttcgccttcc tctcgttcga
     9241 cgcctgacga taccaagcac aacaacagca gcaagtccgc cgcaatgatg ggtcaggcgg
     9301 cctcgagtgc cccagggcgc cagccagggg ctatccacaa gggcctccgg agggaaagct
     9361 ccggtctcac gaagttcgga gcaaagcgcg tcgacgtctc gaacgagaaa gcggccggtg
     9421 ggacggtcgt tggggtaggc ccactgaaac cggtcatgcc actgcaagtg cagttccacg
     9481 ccgtcgcgtg tcacggcggc gtatttcggg tcggcggggt cgtcctgaaa cgcgagggaa
     9541 aacccgaggc gctcgtagaa ccgagtcgag gcagatacgt tgctgctcat gaggaccggg
     9601 tgaacagagt cgagcatggg cgacgttagc gggcgccttc acgacgaggt cgagtgaggc
     9661 cctgaaccgg cccccgagac ccgcctgccg cgcctcgtac caggccaccg agccggattc
     9721 cgcgacgatg ctgtcccaga tggcctgaac gagctcgatt cgttccggaa ccgggaggcg
     9781 aagagcgtcg tcgagtacgg gcgtcgaaat gcagtgatga tacggcggct gacgtctggc
     9841 atcagcggcg agcgtagcga gtccgctgca tgccggggtt aggccg
//
LOCUS       DAOMFY010000134         9704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2133_length_9704_cov_8.068194, whole genome shotgun sequence.
ACCESSION   DAOMFY010000134 DAOMFY010000000
VERSION     DAOMFY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_9704_cov_8.068194"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLB01_11525"
     CDS             <1..212
                     /locus_tag="PLB01_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQQ77976.1"
                     /translation="LPAGTELVDSAHAAADALEAVLPAAARREGGEPGETRILVTDAS
                     DRLRRVAGRFLGRPAADLALVDLPM"
     gene            256..1008
                     /gene="rph"
                     /locus_tag="PLB01_11530"
                     /pseudo
     CDS             256..1008
                     /gene="rph"
                     /locus_tag="PLB01_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
     gene            complement(1041..1802)
                     /locus_tag="PLB01_11535"
     CDS             complement(1041..1802)
                     /locus_tag="PLB01_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ77977.1"
                     /translation="MPAKKSKPTLVLLHGLGGCAADWADAGVPLAKHFAIQALDLPGS
                     FRGPKSEGGYDPAALARWVLGVVPKEPVFLAGHSLGGRVAGEIAALAPGRVAAIALVS
                     PLGAASYGFTDTLKWKAMSRRAVLESVPESSLRSAAAYGFEGDAPGKKAFVARAVEAQ
                     TGPSGKDVAAATEKAVDGVLGAPPLAERLKGTTMPLLVATGERDPLVPPKDARAILKA
                     RPDATFLELPGRGHYALLEDPGRLAEVLRGFFARA"
     gene            1824..1898
                     /locus_tag="PLB01_11540"
     tRNA            1824..1898
                     /locus_tag="PLB01_11540"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1856..1858,aa:Pro,seq:tgg)
     gene            complement(1908..2684)
                     /locus_tag="PLB01_11545"
     CDS             complement(1908..2684)
                     /locus_tag="PLB01_11545"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HQQ77978.1"
                     /translation="MNFFASGVTRVVGHRGSPTKALENTFESFDRAEADGADGFELDV
                     RLTLDGEAVVQHDSEIVLLGRRVPLATVTIPELTELPVERGGYRGFVPTLRDVFLRYG
                     AAGRYLVELKAGPSPRPGLLEFRVAALIAQMHLFDRALVLSFSGDMLRRIREIEPRIE
                     TCLNYDSTARRPDGALWPDLPKGCRAIGPQAGLVTDELFARAKAEGLGVHCWTVNDPA
                     FAAQLARLGAASVISDDVSVVGPAIREVTGAAPPLSLLKA"
     gene            2708..3685
                     /locus_tag="PLB01_11550"
     CDS             2708..3685
                     /locus_tag="PLB01_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77979.1"
                     /translation="MPRAPRPPAEGPFPVPLATIDWLVSRENPGVRAVALRDLLGRPP
                     KDPDLRRARQAAVRDPFLRDLLALVRARLVPSPPVPLERRYDGGMWLALFLTEFGVDE
                     AQPALKHAADVLLARWEKTFVEIERGGGTGDLDVALFSAALRVLARTGRGADPRVVGG
                     AEEIARRSRAGKGRTVKDLLLLGELPERARTDTIRGAIAFLETRLVEAEIPAALAAGA
                     TPEILKAGYPCADETDLFEMLAALKAVRPAGRPVEPNVARALAALAAQADHRARWKLE
                     RSRPERLPLSPERTGDPSRWVTLRALTILQHFRGLAVPSTAPARRAMSK"
     gene            3682..4311
                     /locus_tag="PLB01_11555"
     CDS             3682..4311
                     /locus_tag="PLB01_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77980.1"
                     /translation="MRRSASAPFAACCAAAALAVLAGCSSDPVPGPPRAATHGYRARV
                     TFKTDGIETASYEIAVRGGLRRKGLGDGPALILDTEAKKAWRVAAGGKSASPVEFSEA
                     EKEMPAGIPLTTGFDEKKEAARRNLTLYHREGDEVFAGHACSIWRFDDDPSALNSPTT
                     TYWAANDLDGLVVRWERELPSPGGKGTKYVINLTDVRVGAPPKLFTPPS"
     gene            4386..5240
                     /locus_tag="PLB01_11560"
     CDS             4386..5240
                     /locus_tag="PLB01_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77981.1"
                     /translation="MQLARNLAAALTLLALAVPANGQTPKDPKSPIAGVPDGRGPSGP
                     VTLRIVSPKADDVIAIPPAEAGQPAPTGAPVEVKVELAGYETFQDPATKQGQYVALML
                     DNFPTVFAYYDVAKPWLFKKIPKGTHTLRAFPMRPWGESIKDDTAFASVTFHVGEKDG
                     KNVNEPGAPVLAVVSPRGKVKSASGKVLLDFLVTGCQIADKGAVPSSCRVRYKIDTEK
                     EVTLSKEDAVWLENVAPGKHAWVVGITKDDKIVPGPFTLVQGSFDLEAPGDPAAPPAA
                     PVGKAAGQ"
     gene            5251..6261
                     /gene="dusB"
                     /locus_tag="PLB01_11565"
     CDS             5251..6261
                     /gene="dusB"
                     /locus_tag="PLB01_11565"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQQ77982.1"
                     /translation="MSLVYGGAGTRPCAVDPPLVLAPMAGITDMTYRLLLRRIGGVGL
                     VTMEFVSSEGLTRGNKRTERLLRFDPAERPLSVQIYGADAERMAVAAARVEEMHVDVC
                     DINMGCPANKILKGCSGAGLMRDLGKARAIIAACVKSLPTTPLTVKFRLGINDQTENY
                     LELARICEGEGVAAVALHARTAKQMYTGRADWSKIARLKEAVAIPVVGNGDVNTADDV
                     LAMFRQTGCDAVMCGRATMKNPWIFKQAADLLAGRPWREATLAERRDVILEHFRLIQR
                     DQSDAPKAMMSKLRTFTSWYTHGLPNGSELRRRIASLDGPQAFLDAVEAFFETHAAKV
                     AA"
     gene            complement(6288..7718)
                     /locus_tag="PLB01_11570"
     CDS             complement(6288..7718)
                     /locus_tag="PLB01_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQQ77983.1"
                     /translation="MQVPFRGPAALALGLALAAGPAASAPPPTGTDAYLDATAARWED
                     VSRKIWEYAETALQETKSAALLEEVLEKEGFAVQRGAAGMPTAFVATAGSGAPVVAIL
                     AEYDALPGLSQKAGEAKKSPAVAGAPGQGCGHNLLGTAAVAAAVAANRERIAQKLPGT
                     IRVYGTPAEEQILGKTFMLRDGVFAGTDVVLAWHPEAENYVYSGGRLAITALDVEFFG
                     KTAHAAANPWLGRSSLDALELFEHAMALMREHVHPTARLHRVVKDGGLAANIIPDYAR
                     VQWFVRDATGERMNDMVGRLRKAADGAGLATETTAKVTLLATTREPLYNETISRLVQR
                     HLERIGAPRWDAADQALARAIQKEVGIPEKGLSAEVLPYAKGRGASASSDTGEVSAAY
                     PLIELGVQTAPSGAPWHHWDVASCAAAPMGVKGMLVAAKVLGASTVDLLRDPAAVAAA
                     KAEFAKATAGRPYVSPLAPDAKPKTY"
     gene            7775..9697
                     /locus_tag="PLB01_11575"
     CDS             7775..9697
                     /locus_tag="PLB01_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ77984.1"
                     /translation="MRKSLALASILLALAGAASGQPAPPADAQKSRIDAPGVSVPLDV
                     REQYLGKTGSKTAVRFILSVSRGDLRGPASSPRVVSFTLSGVVSDAKGANVDAFRIPA
                     DVDLGADAGKPVSITFLRPLPPGTYEIQFRLEGVAGRAVATRAVTIAVPEMKAEFRAE
                     DAGTDATGLPSAAAVVLESENRQMPAPNAGNLVKILAPPQEVPVGLLRIGCEVKPPVT
                     RVEFWLEEKKILVKNRAPYEVELDLGKVPKKQTLKALGFDAQGNFLDADAWAINEKDA
                     RLSVRLLELPKQKGAGDTVEIKVAVQSMAGGVAKKLELYLDDRKVKEWNQPPYVAMVP
                     VAQYQKATLIRATATDEDGKEFTDIKLLKGESRFMSKVEVDLVELPVSVYDGEGRLVK
                     TLEKEDFTVTEDAVKQDLTSFEFAESLPLSLGIVIDGSGSMKDAMPIVHQAASEFVQK
                     LVSKEKDQGFVIEFRERPALLAPMTHRAADLERAIAETHANGATALFDSVVMSLYQFR
                     ATPGRKAVVVLTDGDDNHSWSDYPTLRRYARSAGVPIYFIGLNLSFLDTGIKGRMTEL
                     AADTGAEAFFISKASALPEVYRKIETELRSQYSLRYLTNSTKGENQFRAIEVKLRDPK
                     LLRSGQNNSTTQEYAV"
BASE COUNT         1428 a         3494 c         3438 g         1344 t
ORIGIN      
        1 cgctcccggc cggaacggag ctcgtcgact cggcgcacgc cgcggcggac gcactcgagg
       61 cggtgctccc ggcggccgcc cgccgggagg gaggcgagcc cggcgagacg cgcatcctcg
      121 tcacggacgc ttcggaccgg ctccgccgcg tcgcgggccg cttcctcggg cggccggcgg
      181 ccgatctcgc cctcgtcgac ctgccgatgt gacggcccgc gggcggccgg gccgcgataa
      241 gaggacaatc cccgcgtgaa gaaccccaac gccctctccc gtgccggcgg ccgcgccgcg
      301 ctttcgctgc gtcccgtctc gatcgtgtgc gacgccgtgc cccacgcgga aggctcgtgc
      361 ctcatcacgc agggcaagac ccaggtgctc gtcacggcgt ccgtcgaggc gaaggtgccg
      421 ccgttcctcc agaagaccgg cctcggctgg atcacggccg agtacggaat gctcccgcgg
      481 gcgacgcacg agcggaccgc gcgcgaggcc gcgcgcggca agcagacggg ccgaacgctc
      541 gagatccagc gcctcatcgg gcgcgcgctg cggggcgtcg ccgaccgctc gatcctggcg
      601 gaccgcacga tcacgctcga ctgcgacgtc ctcgtcgccg acgcggggac gcgctgcgcg
      661 tccgtgacgg gcgcgtacgt cgcgctcatg ctcgccgccg gcacgctctt caagtcccac
      721 gcgatccgcg ggaacccgct gcgcgaggcc gtcgcggcgg tctcgctcgg cgtcgcggcc
      781 ggaccggcgc cggactcccg cacgatcctc ctcgacctcg actacgccga ggactcgacc
      841 gcggaggtcg acctgaacgt cgtcgggacg tcgtcgggac gcttcgtcga gatccagggc
      901 acggcggaga aggagccttt cgacgaggcg atgctcggcg agctgctcgc cgcggcgcgg
      961 cgcggcctgt ccgagctctt ccgcgtccag cgcgcggcgc tcgagggccg cgtgccggac
     1021 gcgtggctgc cggacctgcc tcaggcccgt gcgaagaacc cgcgcaggac ttcggcgagc
     1081 cggcccggat cttccaagag agcgtagtgc ccccggccgg ggagctcgag gaacgtcgcg
     1141 tccggccggg ccttcaggat cgccctcgcg tccttcggcg gaacgagcgg gtcgcgctcg
     1201 cccgtcgcga cgaggagggg catcgtcgtc cccttcaggc gctcggcgag cgggggcgcg
     1261 ccgaggacgc cgtccacggc cttctcggtc gcggccgcga cgtccttgcc ggacgggccg
     1321 gtctgggcct cgacggcgcg cgcgacgaac gccttctttc cgggcgcgtc gccctcgaag
     1381 ccgtacgccg cggcgcttct cagggacgac tcggggacgc tctcgaggac ggcgcggcgc
     1441 gacatcgcct tccacttgag cgtgtccgtg aagccgtagg acgcggcgcc gaggggagac
     1501 acgagcgcga ttgcggcgac gcgtcccggc gcaagggccg cgatctcccc ggcgacgcgc
     1561 ccgccgaggg agtgccccgc gaggaagacg ggctccttcg gcaccacgcc gagaacccac
     1621 cgcgcgagcg ccgccgggtc gtacccgccc tcggatttcg gcccgcggaa ggagcccggc
     1681 aggtcgagcg cctggatcgc gaagtgcttc gcgagcggga cgccggcgtc tgcccagtcg
     1741 gccgcgcagc cgccgagacc gtggaggagg acgagcgtgg gtttcgactt cttggcgggc
     1801 atcgcggaga atgctaacgg aatcggggcg tagcgcagcg gtagcgcggc tgctttggga
     1861 gcaacaggtc gccggttcga ccccggccgc cccgaccagt cttcccgtca ggccttcagg
     1921 aggctgagcg gcggggcagc accggtgacc tcccggatcg ccggccccac gacggacacg
     1981 tcgtcgctga tgacgctcgc ggcgccgagc cgcgcgagct gcgcggcgaa cgcggggtcg
     2041 ttaacggtcc agcagtggac gccgagcccc tcggccttcg cccgggcgaa gagctcgtcc
     2101 gtgacgaggc cggcctgcgg accgatcgcg cggcagccct tcgggagatc gggccagagc
     2161 gcgccgtcgg gccggcgcgc cgtcgagtcg tagttgaggc acgtctcgat gcgcggctcg
     2221 atctcgcgga tcctccggag catgtcgccc gagaaggaga gcacgagcgc gcggtcgaag
     2281 aggtgcatct gggcgatgag ggccgcgacg cggaactcga ggaggccggg ccgcggcgac
     2341 gggccggcct tgagctcgac gaggtaccgt ccggccgcgc cgtagcgcag gaagacgtca
     2401 cgcagggtcg ggacgaagcc gcggtaaccg cctcgctcga cgggcagctc ggtgagctcg
     2461 gggatcgtca cggtcgccag cgggacgcgg cgtccgagga gaacgatctc ggagtcgtgc
     2521 tggacgacgg cctcgccgtc cagcgtgagg cggacgtcga gctcgaaacc gtcggcgccg
     2581 tcggcctcgg cccggtcgaa gctctcgaaa gtgttttcga gcgccttcgt cggggagccg
     2641 cggtgcccga cgacgcgcgt gacgccgctc gcgaagaagt tcatcggagg gattatcctc
     2701 gcctcccatg cctcgcgctc cgcgtcctcc cgccgagggt ccgttcccgg tcccgctcgc
     2761 gacgatcgac tggctcgtct cgagggaaaa cccgggggtc agggccgtcg cgctgcgcga
     2821 cctgctcggg cgcccgccca aggatcccga cctccggcgc gcccggcagg cggccgtccg
     2881 cgacccgttc ctgcgcgatc tcctcgcgct cgtccgcgcg cggctcgtgc cgtcgccgcc
     2941 ggtcccgctc gagcggcggt acgacggcgg gatgtggctc gcgctgtttc tcacggagtt
     3001 cggcgtcgac gaggcgcagc ccgcgctcaa gcacgcggcc gacgtcctgc tcgcgcggtg
     3061 ggagaagacc ttcgtcgaga tcgagcgcgg cggcgggacg ggcgatctcg acgtcgcgct
     3121 gttctcggcg gcgctccgcg tcctcgcgcg gaccggccga ggcgccgacc cccgcgtcgt
     3181 gggcggggcc gaggagatcg cccggcgctc ccgggccggc aagggccgca ccgtgaagga
     3241 cctcctcctt ctcggagagc tgcccgagcg cgcgcgaacg gacacgatcc ggggcgcgat
     3301 cgcgttcctc gagacccggc tcgtcgaggc ggagatcccc gccgcgctcg ccgcgggcgc
     3361 gacgccggag atcctgaagg cgggctaccc gtgcgcggac gagacggacc tcttcgagat
     3421 gctcgccgcc ctgaaggccg tgcgtcccgc gggccggccc gtcgaaccga acgtcgcgcg
     3481 tgctctcgcg gcgctcgcgg cacaggccga ccaccgggcg cgctggaagc tcgagcgctc
     3541 ccggcccgag cggctgccgc tctctcccga gcgcacgggc gacccgtcac gctgggtcac
     3601 gctgcgcgcg ctcacgatcc tccagcactt tcgcgggctc gccgtcccga gcacggctcc
     3661 cgcccggagg gccatgtcga aatgagacgc tccgcttccg cccctttcgc cgcctgctgt
     3721 gccgcggcgg ccctcgccgt tctcgccggc tgctcgtccg atcccgtccc cgggccgccg
     3781 cgcgccgcga cccacggcta tcgcgcccgt gtcacgttca agacggacgg gatcgaaacg
     3841 gcgtcgtacg agatcgccgt gcgcggaggc ctgcggcgca agggcctcgg cgacggcccc
     3901 gcgctgatcc tcgacacgga ggcgaagaag gcgtggcggg tcgccgcggg cgggaagagc
     3961 gcgtcgcccg tggagttctc cgaagccgag aaagagatgc ccgccggcat cccgctaacg
     4021 acgggcttcg acgagaagaa ggaagcggcc cgccggaacc tcaccctcta ccaccgcgag
     4081 ggcgacgagg tgttcgccgg ccacgcgtgc tcgatctggc gcttcgacga cgacccgtcc
     4141 gccctgaatt cgccgacgac gacgtactgg gccgcgaacg acctcgacgg cctcgtcgtg
     4201 cgctgggagc gggagctccc gtcgccgggc ggcaagggga cgaagtacgt gatcaacctc
     4261 acggacgtgc gcgtcggggc cccgccgaag ctcttcacgc cgccctcctg agacacgggc
     4321 gcagcgtcgc tgctatgctc cgcgccctcc ggcgcgccgg aggatcctcc agaggagtgt
     4381 ttccgatgca gctcgcccga aacctcgcgg ccgctctcac cctcctggcg cttgccgtcc
     4441 cggcaaacgg ccagacgccg aaggacccga agtcgccgat cgccggagtt ccggacgggc
     4501 gcggaccttc gggccctgtc acgctccgga tcgtgtcgcc gaaggcggac gacgtgatcg
     4561 cgatcccgcc cgctgaagcc gggcagcccg cccccacggg cgcgcccgtc gaggtcaagg
     4621 tcgagctcgc gggatacgag acgttccagg accctgcgac gaagcaggga cagtacgtcg
     4681 ccctcatgct cgacaacttc ccgaccgtct tcgcctacta cgacgtcgcg aagccctggc
     4741 tcttcaagaa gatcccgaag ggcacccaca cgctccgcgc gttcccgatg cggccgtggg
     4801 gcgagtccat caaggacgac accgcgttcg cgtccgtcac gttccacgtc ggcgagaagg
     4861 acgggaagaa cgtgaacgag cccggcgcgc cggtcctcgc ggtcgtctcg ccgcgcggga
     4921 aagtcaagtc ggcgtccggc aaggtcctgc tcgactttct cgtgaccggc tgccagatcg
     4981 ccgacaaggg cgcggttccg agcagctgcc gcgtgcgcta caagatcgac accgagaagg
     5041 aagtcacgct ctcgaaggaa gacgccgtgt ggctcgagaa cgtcgcgccg ggcaaacacg
     5101 cgtgggtcgt cgggatcacg aaggacgaca agatcgttcc cggcccgttc acgctcgtgc
     5161 agggctcctt cgatctcgag gctccgggcg accccgccgc accccccgcg gcgcccgtcg
     5221 gaaaggccgc gggccagtaa aatcccccgg atgagcctcg tctacggcgg cgcggggacg
     5281 cgtccctgcg ccgtcgatcc ccccctcgtc ctcgcgccca tggcggggat cacggacatg
     5341 acgtaccggc tgctcctgcg ccggatcgga ggggtgggcc tcgtcacgat ggagttcgtc
     5401 tcgtccgagg ggctcacgcg cggcaacaag cggacggagc gcctcctccg cttcgacccg
     5461 gcggagcgtc cgctctcggt ccagatctac ggagcggacg ccgagcggat ggcggtcgcc
     5521 gcggcccgcg tcgaggagat gcacgtcgac gtctgcgaca tcaacatggg ctgcccggcg
     5581 aacaagatcc tcaagggctg ttccggggcc ggcctcatgc gcgacctcgg caaggcgcgc
     5641 gcgatcatcg cggcctgcgt caagtcgctc ccgacgacgc ccctcacggt gaagttccgg
     5701 ctcggaatca acgaccagac ggagaactac ctcgagctcg cgcgcatctg cgagggcgag
     5761 ggcgtggcgg ccgtcgcgct tcacgcccgc acggcgaagc agatgtacac gggccgcgcg
     5821 gactggtcga agatcgcgcg tctcaaggaa gccgtcgcga ttcccgtcgt cgggaacggc
     5881 gacgtgaaca ccgccgacga cgtgctcgcg atgttccgcc agacgggctg cgacgccgtc
     5941 atgtgcggcc gcgcgacgat gaagaacccg tggatcttca agcaggccgc cgacctcctc
     6001 gcgggccggc cgtggcgcga ggcgacgctc gccgagcggc gcgacgtcat cctcgagcac
     6061 ttccgcctca tccagaggga ccagtcggac gcgccgaagg cgatgatgtc gaagctccgc
     6121 acgttcacgt cgtggtacac gcacggcctc ccgaacggct cggagctgcg ccgccgcatc
     6181 gcgagcctcg acgggccgca ggcgttcctc gacgccgtcg aggcgttctt cgagacgcac
     6241 gcggcgaaag tcgcggcctg agggctcgcg cgccgcggcc tgagcgctca gtacgtcttc
     6301 ggtttcgcgt ccggggcgag ggggctgacg tagggccttc cggccgtcgc cttcgcgaat
     6361 tcggccttcg cggccgcgac cgcggcggga tccctgagga gatccaccgt cgacgccccg
     6421 agcaccttcg cggcgacgag catgcccttg acgcccatcg gggccgcggc gcaggacgcg
     6481 acgtcccagt ggtgccacgg cgctccgctc ggcgccgtct ggacgccgag ctcgatgagc
     6541 gggtacgcgg cgctcacctc gcccgtgtcg gacgacgcgg aggcgcctcg gcccttcgcg
     6601 tacgggagga cctcggccga gagccccttc tccgggatcc cgacctcctt ctggatcgct
     6661 cgtgcgaggg cctggtcggc tgcgtcccac ctcggggcgc cgatgcgctc gagatgcctc
     6721 tgaacgagcc tcgagatcgt ctcgttgtag agcggttcgc gcgtcgtggc gagcagggtc
     6781 actttcgccg tcgtctccgt cgcgagaccg gcgccgtccg cggccttcct cagccggccg
     6841 accatgtcgt tcatgcgctc gccggtcgcg tcgcgcacga accactgcac gcgggcgtag
     6901 tccgggatga tgttcgcggc gaggccgccg tccttcacga cgcggtgaag gcgcgccgtc
     6961 gggtggacgt gctcgcgcat gagcgccatc gcgtgctcga agagctctag agcatccaaa
     7021 gagcttcgac ccagccaggg gttcgcggcg gcgtgcgccg tcttcccgaa gaactcgacg
     7081 tcgagggccg tgatcgcgag gcgcccgccc gagtagacgt agttctcggc ctccgggtgc
     7141 cacgcgagga cgacgtccgt cccggcgaag acgccgtccc gcagcatgaa cgtcttgccg
     7201 aggatctgct cctcggcggg cgtcccgtag acccggatcg tgccggggag cttctgcgcg
     7261 atccgttcgc ggttcgcggc gacggcggcc gcgacggcgg cggtcccgag aaggttgtgt
     7321 ccgcacccct ggcccggcgc gccggcgacg gcgggggact tcttcgcctc gccggccttc
     7381 tgcgagaggc ccggaagcgc gtcgtactcg gcgaggatcg cgacgacggg cgctcccgac
     7441 cccgcggtcg cgacgaacgc cgtcggcatc ccggctgcgc cccgctgcac ggcgaagcct
     7501 tccttttcga ggacctcctc gaggagcgcc gccgacttcg tctcctgcag ggccgtttcg
     7561 gcgtactccc agatcttccg ggacacgtcc tcccagcgcg ccgcggtcgc gtcgaggtac
     7621 gcatcggtgc cggtgggcgg cggggcggag gcggccggac cggccgcgag agcgaggccg
     7681 agcgcgagag cggcggggcc gcggaaagga acttgcatcg ggagcctccg aacgggggat
     7741 gtgtcgcttg aggaagacag catacgatgg gggaatgcga aagagtctcg cgctggcgtc
     7801 gatccttctc gccctcgcag gggccgcatc cggtcagccg gctccccctg cggacgccca
     7861 gaagagccgg atcgacgccc ccggagtctc cgttcccctc gacgtgcgcg agcagtacct
     7921 cggaaagacg gggtcgaaga cggccgtccg gttcatcctc tccgtttcgc gcggcgacct
     7981 ccggggtccc gcgagctcgc cgcgcgtcgt ttcgttcacg ctgtcgggcg tcgtgagcga
     8041 cgcgaagggc gcgaacgtcg acgccttccg gatcccggcc gacgtcgacc tcggcgccga
     8101 cgcgggcaag cccgtttcga tcacgttcct gcgcccgctg ccgccgggga cgtacgagat
     8161 ccagttccgc ctggaaggcg tcgccggccg tgcggtcgcg acgcgcgccg tgacgatcgc
     8221 cgtgccggaa atgaaagccg agttccgcgc cgaggacgcc ggcacggatg cgaccggcct
     8281 gccgagcgcg gcggccgtcg tcctcgagtc cgagaaccgc cagatgccgg ccccgaacgc
     8341 ggggaatctc gtgaagatcc tcgcgccccc ccaggaggtc cccgtcggcc tcctccggat
     8401 cggctgcgag gtcaagccgc ccgtcacgcg cgtcgagttc tggctcgagg agaagaagat
     8461 cctcgtgaag aaccgcgccc cctacgaggt cgagctcgac ctcggcaagg tcccgaagaa
     8521 acagacgctc aaggcgctcg gcttcgacgc gcaggggaat tttctcgacg ccgacgcgtg
     8581 ggcgatcaac gagaaggacg cgcgcctgtc cgtgcgcctc ctcgagcttc cgaagcagaa
     8641 gggggcgggc gacacggtcg agatcaaggt cgccgtccag tccatggcgg gcggcgtcgc
     8701 gaagaagctc gagctctacc tcgacgaccg gaaggtcaag gagtggaacc agccgcccta
     8761 cgtcgcgatg gtgcccgtcg cccagtacca gaaggcgacg ctgatccgcg cgacggccac
     8821 ggacgaggac gggaaggaat tcacggacat caagctcctc aagggcgagt cgcgcttcat
     8881 gtcgaaggtg gaggtcgacc tcgtcgagct gccggtctcc gtctacgacg gggagggccg
     8941 cctcgtgaag accctcgaga aggaggactt caccgtcacg gaggatgcgg tcaagcagga
     9001 tctcacgagc ttcgagttcg ccgagtcgct cccgctctcc ctcgggatcg tgatcgacgg
     9061 atcgggctcg atgaaggacg cgatgccgat cgtccaccag gcggcgtccg agttcgtcca
     9121 gaagctcgtc tccaaggaga aggaccaggg gttcgtgatc gagttccggg agcgccccgc
     9181 cctcctcgcc ccgatgacgc accgcgcggc ggacctggag cgcgcgatcg ccgagacgca
     9241 cgcaaacggc gcgaccgccc tcttcgactc cgtggtcatg tcgctctacc agttccgtgc
     9301 gacgcccggc cgcaaggcgg tcgtcgtgct gacggacggc gacgacaacc actcctggag
     9361 cgactacccg acgctccggc gctacgcgcg ctcggcggga gtcccgatct acttcatcgg
     9421 gctgaacctc tcgttcctcg acacgggcat caaggggcgc atgacggagc tcgcggccga
     9481 cacgggtgcc gaagcgttct tcatctcgaa ggcgtcggcc ctgccggagg tctaccggaa
     9541 gatcgagacg gagctgcgct cgcagtattc cctcaggtat ctcacgaact ccacgaaggg
     9601 cgagaaccag ttccgcgcga tcgaggtgaa gctcagggac ccgaagcttc tccggagcgg
     9661 gcagaataat agcacaactc aggaatacgc tgtctaaaaa tcct
//
LOCUS       DAOMFY010000135         9648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2147_length_9648_cov_6.485250, whole genome shotgun sequence.
ACCESSION   DAOMFY010000135 DAOMFY010000000
VERSION     DAOMFY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2147_length_9648_cov_6.485250"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..578
                     /locus_tag="PLB01_11580"
     CDS             270..578
                     /locus_tag="PLB01_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77985.1"
                     /translation="MPPRVRGLLLVGLGLLALKGAALFFAWRGGLSSELDPEESHPLG
                     ILFVPGLLVALVGVVQTVLGRPFGQWQGAWDEMPEWGQRIVVYGILFGTPIALYLFFR
                     "
     gene            complement(601..1449)
                     /gene="ispH"
                     /locus_tag="PLB01_11585"
     CDS             complement(601..1449)
                     /gene="ispH"
                     /locus_tag="PLB01_11585"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQQ77986.1"
                     /translation="MDVVLAEKYGFCAGVRVADKLVRLAAQRGETGAILGQVVHNESV
                     ESEMAGLGFPTVHALEDARTHAGRIVFSAHGVAPSVRARAAELGLTAIDTTCKFVTDI
                     HKEIARSLAEGAFLAILGQADHREVVGYTKDLDPQRYKVFYRLEDVKAFDWSRTTVVK
                     VIFQTTINSETFAEHVAEIRRRVPDTRIADTICYATKENQDALRVLCDDPTVDAIVVI
                     GGKNSKNTKELAHIAAEKKPTLLVGTADELDPAKLAGVRKVGVSAGASTPDYDVEAVV
                     AKLKSL"
     gene            complement(1449..2309)
                     /locus_tag="PLB01_11590"
     CDS             complement(1449..2309)
                     /locus_tag="PLB01_11590"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQQ77987.1"
                     /translation="MRATAASALFTFIGFSSSAAPVELPRPSPAASVFEKIGTAKLTV
                     AYSRPAVKGREIWGALVPYGQVWRLGANDATTLEISEACKLAGHDLPAGAYALFAIPA
                     KDKWTIVVNSQAKQWGAYFRDPKKDVFTFDVTPTAGPHVEWLEYRMHPESARVVRVEM
                     TWEKLRISFPVEVDVNGIVWKNLDAARAAAGPKDYVDFYQSARFSRETGERKAEAMGW
                     LDEAMKRGDSFWMDELKGDLLADAGKYAEAAPWLEKAIEGSKKAGAPEEWRDGARKKL
                     SEWAAKAKKG"
     gene            complement(2409..4721)
                     /locus_tag="PLB01_11595"
     CDS             complement(2409..4721)
                     /locus_tag="PLB01_11595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HQQ77988.1"
                     /translation="MTRKHSAASGGQPPLPFDAEVALPEEARRRYLNYALSVITSRAL
                     PDVRDGLKPVQRRILFAMFQNLHLMPDAKFKKSASVVGDVIGKYHPHGDVAIYDAMVR
                     MAQPFSLRAPLVDGHGNFGSLDGDSAAAYRYTEARLKPLAIELLSEIRKKTVDWRPNF
                     DGVHFEPVVLPARFPNLLVNGATGIAVGMATSIPPHNPVEVLDAAIAAVDDADSDPLK
                     HIKGPDFPTGGLLLGGKREMRALYESGQGTLKLRAEWEVEEGSRGAQTIVITSIPYAQ
                     EKASLVSKIADVIIERRLPVLVDVRDESTDDVRIVLEIKRDTDPALVMTYLYKHTPLE
                     TTVPINLTCLVPGPNPDVGEPARLSLKAMLRHFLDFRFETVRRRFTFDLEELRRRIHL
                     LEGFVVIYDALDEAIRIIRKSDGKADAAGKLMKRFGLDEEQVEAILETKLYKLARLEI
                     DAIRAELKEKLAEAAIIEAILKSPKKLWNEVKGELGEVRESLAGEKRRTKVQASVDEP
                     EYDAEAFIQHEDTYAVVTVDGWLKRLGQLKDPKATRLREGDEVLAVLQGSTKELGVLF
                     SNHGAAYVLRLNDVPPSTGYGEPVQKLFKFGDGERVIAALSLDPRISRPKNLTLVAVT
                     RRGLAFRFSADVLREVTTRAGRRYARPAKGDEVAHVFVCEEKDLVALASEKGRAILFP
                     ASEVPVLSGPGKGVLGFKLAPDDRLVGAAVFGEDEKRATLTLVNGKGTEHTVTKRYTP
                     VSRGGKGFELIKRDRLVKMLPQDIELIPLG"
     gene            4847..5410
                     /locus_tag="PLB01_11600"
     CDS             4847..5410
                     /locus_tag="PLB01_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ77989.1"
                     /translation="MDAASDDLFARIVAGDREAFAAFYDLHAPVLFGFCVRILKDARD
                     AEDVLQETFVQAWRDAGRFDAARASVRGWLFTIARSRALDRWRSRRSFDKRISAADTP
                     ILEQAAGAADHTAAADVRDFVERALGQLNENEQAVLRLAYFEGLTQEEIAARLSEPLG
                     TVKSRTRSGLSKMRTIAARVQKEAGRV"
     gene            5403..6137
                     /locus_tag="PLB01_11605"
     CDS             5403..6137
                     /locus_tag="PLB01_11605"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HQQ77990.1"
                     /translation="MSDERTRALRAAEDAAFQAECLEAELDALDTGARIEPPAEVRDR
                     VLWEIRGPGAEAAARAIAEAGPDRGLATMIWALGVAASFMLAAAFGALWYATRADLAA
                     TRENVARLETMVKERDRQISLTSQEVSWLKDPRVQVALLKGLEGNAAARAKLLWNPGT
                     KQGILWVSGLPPLPLEKSYELWAFVGDQPVPAGTFDAKADGATVIPISRQESFDDRPV
                     KFAVSVEPKGGVPAPTGAIVLVGERF"
     gene            6325..6750
                     /locus_tag="PLB01_11610"
     CDS             6325..6750
                     /locus_tag="PLB01_11610"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HQQ77991.1"
                     /translation="MILAGALAGSAALAQPGPGPNRMAQGIRAAMATLDLSDAQKEKI
                     KAIFESHKEEGAALRAQAKADRDALKAAASAAKPDPAVVGAAFLKVRADGETAKAKMK
                     AVRAEIDAVLSPEQRAKLDGWIAAHRQMRGGLRGGPPQN"
     gene            complement(6754..>7116)
                     /locus_tag="PLB01_11615"
     CDS             complement(6754..>7116)
                     /locus_tag="PLB01_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HQQ77992.1"
                     /translation="LDRLASGTLALLHEDIRPYFRDVNDHLVRVIEQLEGDRDLLTSI
                     LQSNLTQVSILQNEDMRKISSWAAIFAVPTMIAGIYGMNFQHMPELKSPWGYPFALGL
                     MLVVCSGLYRAFKRSGWL"
     assembly_gap    7117..7216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7217..7515)
                     /locus_tag="PLB01_11620"
     CDS             complement(<7217..7515)
                     /locus_tag="PLB01_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HQQ77993.1"
                     /translation="MIVDCGIYDGGVRRAGTFTFEEAYEASKRPDSFVWLGLYEPTPE
                     EFASVKTAFELHELAVEDALKAHQRPKLEVYDDTLFIVVKTARYVDSDEVVSLGEL"
     gene            complement(7526..9268)
                     /gene="ade"
                     /locus_tag="PLB01_11625"
     CDS             complement(7526..9268)
                     /gene="ade"
                     /locus_tag="PLB01_11625"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HQQ77994.1"
                     /translation="MSDLPEALPLCDFVAAARGVVPADLVLRNARVVNVLTGEIHDGT
                     VGVKGDRILGLGDYRTEREEDTVDLRGAYVAPALWDAHLHVESTMTTPGTFARHAAVH
                     GTAAAVTDPHEIANVRGVAGIRDFLAAAAGLPVDVFVMLPSCVPSTHLETAGAALSAD
                     DLAPLYNLPNVLGLAELMNVPGFLFGDPGVLAKVEDARRRRVPIDGHSPLLAGLDLNA
                     YVGAGIRSDHECLSRAEAHEKVRRGMDIWIREGTAAKNLDELLPLVTAANSERFAFAT
                     DDRHPWDLLRHGHLDHHVRRAIALGLDPVLALRLASWSCARHYGFTDRGAVAPGFRAD
                     LLTFERLDDFRPDLVVLGGRRVAKGGQLLVDVPAARMEEGPGFRVEGFDRTRLRVAAG
                     EGASLRVIEVRAGSIATGASNERAALAEGAAVPDVSRDLLKLAVVERHRGTGNVGVAF
                     ARGFGLKRGAVASSVAHDSHNVVVVGADDASMETAVRRVAAIGGGIVVADGAQALAEV
                     ALPVGGLMSNGSMADVDAAERRANAAAATLGGTMDHPFMTLAFLALPVIPELKLTDKG
                     LVDVAKFDFVAPVE"
     gene            9398..>9648
                     /locus_tag="PLB01_11630"
     CDS             9398..>9648
                     /locus_tag="PLB01_11630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQQ77995.1"
                     /translation="MVDREVERSRRHGSVLSVLSIVLANWNDVTCAAPVPVVDDAVRT
                     VANTLLANLRMTDYLFRWEDDEFLALLVEANLDGCKLKVA"
BASE COUNT         1394 a         3346 c         3358 g         1450 t
ORIGIN      
        1 cgcccaccgt cgtcccgtcc ccgaccgtcg tgccgtcccc cacgccgacg cggcgcgcga
       61 ccacgactcc cgtcccgact ccgaggccct cggccggcga ggacgggtgg cacccgaagc
      121 ggcgctagga gccgcgcgcg agtcccgggc cgcggggtcc cgggcgtgat ggaatctgcc
      181 tcccttttcc gcctttaggg aagggaggca cggtgtcatc cgaacgagtc cctcgtaaga
      241 ctagcgccgc cgcagctgcc tggcgacgcg tgccgccccg cgtgcggggg ctcctcctcg
      301 tcggcctcgg cctgctcgcc ctgaaaggcg ccgccctctt cttcgcctgg cgcgggggac
      361 tttcgtcgga gctggacccc gaggaaagcc accctctcgg aatcctgttc gtgcccggcc
      421 tgctcgtcgc gctcgtggga gtcgtccaga ccgtcctcgg acgtccattc ggccagtggc
      481 agggcgcctg ggacgagatg cccgagtggg gacagcggat cgtcgtctac gggatcctct
      541 tcggcactcc gatcgccctc tacctcttct tccgctgatg ccggcggcgc ccccggcagc
      601 tcagaggctc ttcagcttcg cgacgaccgc ctcgacgtcg tagtccggcg tcgaggcgcc
      661 cgcggaaacg ccgacctttc gcacgccggc gagcttcgcg ggatcgagct cgtcggcggt
      721 gccgacgagg agcgtcggct tcttctccgc ggcgatgtgg gcgagctcct tcgtgttctt
      781 gctgttcttc ccgccgatga cgacgatcgc gtcgaccgtc gggtcgtcgc agaggactcg
      841 gagggcgtcc tggttctcct tcgtcgcata gcagatcgtg tccgcgatgc gcgtgtccgg
      901 gacgcggcgc cggatctcgg ccacgtgctc ggcgaacgtc tcggagttga tcgtcgtctg
      961 gaagatgacc ttgacgacgg tggtgcgcga ccagtcgaac gccttcacgt cctcgagcct
     1021 gtagaagacc ttgtagcgct gcgggtcgag gtccttcgtg tagccgacga cctcgcggtg
     1081 gtcggcctgg ccgaggatcg cgagaaacgc gccctcggcg agcgagcggg cgatctcctt
     1141 gtggatgtcc gtcacgaact tgcacgtcgt gtcgatcgcc gtaaggccga gctccgcagc
     1201 gcgcgcccgc acagacgggg cgacgccgtg cgccgagaag acgatccggc cggcgtgcgt
     1261 gcgggcgtcc tcgagcgcgt ggaccgtcgg gaacccgagg cccgccatct cggactcgac
     1321 gctctcgttg tggacgacct ggccgaggat cgcacccgtt tcgccgcgct gcgccgcgag
     1381 gcgcacgagc ttgtccgcga cgcgcactcc cgcgcagaag ccgtacttct cggccaggac
     1441 gacgtccatc agcccttctt tgccttcgcg gcccactcgg agagcttctt ccgcgcgccg
     1501 tcgcgccact cctcgggcgc gccggccttc ttcgagccct cgatggcttt ctcgagccag
     1561 ggcgccgcct cggcgtactt ccccgcgtcc gcgaggaggt cgcccttgag ctcgtccatc
     1621 cagaagctgt cgccacgctt catcgcctcg tccagccagc ccatcgcctc ggccttgcgc
     1681 tcgcccgtct cgcgggagaa gcgcgccgac tggtagaagt ccacgtagtc cttcgggccg
     1741 gccgcggcgc gggccgcgtc gaggttcttc cagacgatcc cgttgacgtc cacttcgacc
     1801 gggaaggaga tccgcagctt ctcccacgtc atctcgaccc tcacgacgcg cgcggactcc
     1861 gggtgcatcc ggtattccag ccactccacg tgcggcccgg cggtcggcgt cacgtcgaac
     1921 gtgaacacgt ccttcttcgg gtcgcggaag tacgcgcccc actgcttcgc ctgcgaattc
     1981 acgacgatcg tccacttgtc cttcgcgggg atcgcgaaga gcgcgtaggc acccgccgga
     2041 aggtcgtggc cggccagctt gcaggcctcc gagatctcca gcgtcgtcgc gtcgttcgcg
     2101 ccgagccgcc agacctgccc gtacgggacg agagcgcccc agatctcgcg gcccttcacg
     2161 gccgggcggg agtacgcgac ggtgagcttc gcggtcccga tcttctcgaa cacggacgcg
     2221 gccgggctcg ggcgcggcag ctcgacgggg gccgcggaag aagaaaagcc tatgaaggtg
     2281 aagagggcgg aggcggcggt cgcccgcacg accgagccca tcctcgaacc gacggctgct
     2341 ttccctgccc tattcacttt catagaaatc ccccgtcctc ctcgatctta tcgtctagca
     2401 acagcgtctc aaccaagagg aatcagctcg atgtcctgcg ggagcatctt cacgaggcgg
     2461 tcgcgcttga tgagctcgaa gcccttcccg ccgcgggaca cgggcgtgta gcgcttcgtg
     2521 acggtgtgct cggtgccttt gccgttcacg agcgtgagcg tcgcgcgctt ctcgtcttcg
     2581 ccgaacacgg ccgcgccgac gagccggtcg tccggcgcga gcttgaagcc gaggacgccc
     2641 ttgcccggcc ccgagaggac ggggacctcg gacgccggga acaggatcgc ccggcctttc
     2701 tcggacgcga gagccacgag gtccttctcc tcgcagacga agacgtgcgc gacctcgtcg
     2761 cccttggccg ggcgcgcgta gcgccgtccg gcgcgcgtcg tgacctcgcg caggacgtcc
     2821 gccgagaatc ggaaggcgag cccgcggcgg gtgacggcga cgagcgtgag gttcttcggg
     2881 cgcgagatgc gcgggtcgag ggagagcgcc gcgatcacgc gctcgccgtc cccgaacttg
     2941 aagagcttct ggacgggctc gccgtatccg gtcgacggcg ggacgtcgtt gaggcggagg
     3001 acgtaggccg cgccgtggtt cgagaagagg acgccgagct ccttcgtcga gccctgcagg
     3061 acggcgagga cctcgtctcc ctcgcgcaag cgcgtcgcct tcgggtcctt cagctggccg
     3121 aggcgcttga gccagccgtc gacggtgacg acggcgtagg tgtcctcgtg ctggatgaag
     3181 gcttcggcgt cgtactccgg ctcgtcgacg ctggcctgca ccttcgtgcg ccgcttctcg
     3241 ccggcgagag actcgcggac ctcgccgagc tcgcccttca cctcgttcca gagcttcttc
     3301 ggcgacttga ggatcgcctc gatgatcgcg gcctccgcga gcttctcctt cagctcggcg
     3361 cggatcgcgt cgatctccag gcgcgcgagc ttgtagagct tcgtctcgag gatcgcctcg
     3421 acctgctcct cgtcgaggcc gaagcgcttc atgagcttgc cggccgcgtc cgccttcccg
     3481 tccgacttgc ggatgatccg gatcgcctcg tcgagggcgt cgtagatgac gacgaacccc
     3541 tcgaggaggt ggatccgtcg gcgcagctcc tcgaggtcga acgtgaagcg gcggcgcacc
     3601 gtctcgaacc ggaagtcgag gaagtgccgg agcatcgcct tgagcgagag gcgcgcgggc
     3661 tcgccgacgt ccgggttcgg gccggggacg aggcacgtga ggttgatcgg caccgtcgtc
     3721 tcgagcggcg tgtgcttgta gaggtacgtc atgacgagcg cgggatccgt gtcgcgcttg
     3781 atctcgagga cgatgcgcac gtcgtccgtc gactcgtcgc gcacgtcgac gaggaccgga
     3841 agccggcgct cgatgatgac gtccgcgatc ttcgagacga gggaggcctt ctcctgcgcg
     3901 tacgggatag acgtgatcac gatcgtctgc gcgccgcgcg agccctcctc gacctcccac
     3961 tcggcgcgca gcttgagcgt gccctgcccg ctctcgtaga gcgcccgcat ctcgcgcttg
     4021 cccccgagga gaaggccgcc cgtcgggaag tccggcccct tgatgtgctt gagcgggtcg
     4081 gagtccgcgt cgtcgaccgc cgcgatcgcc gcgtcgagga cctcgaccgg gttgtgcggc
     4141 gggatcgacg tcgccatgcc gaccgcgatg ccggtcgcgc cgttcacgag gaggttcggg
     4201 aaccgcgcgg gcaggacgac cggctcgaag tggacgccgt cgaagttcgg gcgccagtcg
     4261 accgttttct tgcggatctc ggagaggagc tcgatcgcga gcggcttgag gcgcgcctcc
     4321 gtgtaccggt aggcggcggc cgagtcgccg tcgagcgagc cgaagttgcc gtgcccgtcc
     4381 acgagcggcg cgcgcagcga gaagggctgc gccatgcgga ccatcgcgtc gtagatcgcg
     4441 acgtcgccgt gcggatggta cttgccgatc acgtcgccga cgaccgacgc ggacttcttg
     4501 aacttcgcgt ccggcatcag gtgcaggttc tggaacatcg cgaacaggat ccggcgctgg
     4561 acgggcttga ggccgtcgcg gacgtcgggc agcgcgcgcg aggtgatgac ggacagggcg
     4621 tagttcaggt accgccgccg ggcttcctcg gggagggcga cctccgcgtc gaagggcagg
     4681 ggcggctggc cgccggaagc ggcggaatgc ttgcgtgtca tgcgtgctcc gggcgcgcgc
     4741 tgcgcgcctc gggaggcaag gtagcggggc gcggcgaggc cgtcaaatgg cctcctcctc
     4801 cgctcccgct tgacaggccc gggcggcccg gtcagtctgc cggggcgtgg atgcggcgtc
     4861 ggacgacctc ttcgcgcgta tcgtcgcggg agaccgcgag gcgttcgccg cgttctacga
     4921 cctccacgca ccggtcctat tcgggttctg cgtccgtatc ctgaaggacg cgagggacgc
     4981 cgaagacgtg ctgcaggaga ctttcgtcca ggcctggcga gacgcgggcc gcttcgacgc
     5041 cgcccgcgcc tccgtgcggg gctggctgtt cacgatcgcg cgcagccggg cgctcgaccg
     5101 ctggcgcagc cgccggtcgt tcgacaagcg gatctcggcg gcggacacgc ccatcctcga
     5161 gcaggccgca ggcgccgccg accacaccgc ggcggccgac gtgcgcgact tcgtcgagcg
     5221 cgcgctcggc cagctgaacg agaacgagca ggcggtcctc cgcctcgcgt acttcgaagg
     5281 cctgacgcag gaagagatcg ccgcgcgcct ttccgagccg ctcgggaccg tcaagagccg
     5341 gacgcgctcc ggcctttcca agatgcgcac gatcgccgcg agggtgcaga aggaggccgg
     5401 ccgtgtctga cgagcgcacg cgcgccctcc gcgccgcgga agacgccgcc ttccaggccg
     5461 agtgcctcga ggccgagctc gacgcgctcg acacgggcgc gaggatcgag ccgcccgccg
     5521 aggtccggga ccgcgtcctc tgggagatcc gggggccggg cgccgaggcc gcggcgcgcg
     5581 ccatcgccga ggccgggccg gaccggggcc ttgcgacgat gatctgggcg ctcggggtcg
     5641 ccgcgtcgtt catgctcgcg gccgcgttcg gcgccctctg gtacgcgacg cgggccgacc
     5701 tcgcggcgac gcgcgagaac gtcgcgcgcc tcgagacgat ggtgaaggag cgcgaccggc
     5761 agatctcgct gacgtcgcag gaagtctcct ggctgaagga cccgcgcgtg caggtcgcgc
     5821 tcctgaaggg gctcgaggga aacgcggccg cccgcgccaa gctgctctgg aatcccggga
     5881 cgaagcaggg aatcctctgg gtgtccggcc tgcctcccct gccgctcgag aagagctacg
     5941 agctgtgggc gttcgtcggc gaccagcccg tgcccgcggg gacgttcgac gcgaaggccg
     6001 acggggcgac cgtgatcccc atctcgaggc aggagtcgtt cgacgaccgg cccgtgaagt
     6061 tcgccgtctc cgtggagccg aagggcggcg tccccgcccc cacgggcgcc atcgtcctcg
     6121 tcggcgagcg gttctgagcg ttccgaacgg agaggtccga agaggacgag gatcttctta
     6181 gaaggttaag aggggaaagt ccgcatctgt aaacgccggc cccacgttcg gcgtctgttc
     6241 tgacggacgg aaaagaaagc cgtccggagg atcgaaaaaa tgtcacctaa accctttcaa
     6301 aagagaatct tcggcgccgc ttccatgatt ctcgccggcg ccctggccgg ctccgccgcc
     6361 ctcgcccagc cgggacccgg gccgaacagg atggcccagg gcatccgcgc ggcgatggcg
     6421 accctcgacc tttcagacgc ccagaaggag aagatcaagg cgatcttcga atcccacaag
     6481 gaagagggcg cggccctccg cgcgcaggcc aaggccgacc gcgacgccct gaaggcggcc
     6541 gcgtccgccg ccaagcccga cccggcggtc gtcggcgccg cgttcctcaa ggtgagggcc
     6601 gacggcgaaa ccgccaaggc gaagatgaag gccgtccgcg ccgagatcga cgcggtcctc
     6661 tcgcccgagc agcgggcgaa gctggacggc tggatcgccg cccaccgcca gatgcgcggc
     6721 gggctccgcg gcggaccgcc gcagaactga tcgctagagc cagcccgacc gcttgaacgc
     6781 gcggtagagc cccgagcaga cgaccagcat gagcccgagc gcgaagggat atccccacgg
     6841 ggacttcagc tcgggcatgt gctggaagtt catgccgtag atccccgcga tcatcgtcgg
     6901 caccgcgaag atcgcggccc acgacgagat cttccgcatg tcctcgttct ggagaatcga
     6961 gacctgcgtc aggttcgact gcaggatgct cgtcaggagg tcgcggtccc cctcgagctg
     7021 ctcgatgacg cgcacgaggt ggtcgttcac gtctcggaag tagggccgga tgtcctcgtg
     7081 gaggagcgcg agcgttccgc tcgcgagccg gtccagnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnagct cgccgagcga cacgacctcg tcggaatcca cgtagcgggc
     7261 cgtcttgacc acgatgaaga gggtgtcgtc gtagacctcg agcttcgggc gctggtgggc
     7321 cttcagagcg tcctcgaccg cgagctcgtg gagctcgaac gcggtcttga cggacgcgaa
     7381 ctcctcgggg gtgggctcgt agagccccag ccacacgaac gagtcgggac gcttcgacgc
     7441 ctcgtaggcc tcttcgaacg tgaacgtgcc cgcgcgccgc acgccgccgt cgtagatgcc
     7501 gcagtcgacg atcacgcgat ccctctcatt ccaccggcgc cacgaagtcg aacttcgcga
     7561 cgtccacgag gcctttgtcc gtgagcttga gctccgggat caccggcagc gcgaggaagg
     7621 cgagcgtcat gaaggggtgg tccatcgtgc ccccgagcgt cgcggcggcc gcgttcgccc
     7681 gccgctcggc ggcgtcgacg tcggccatgg agccgttcga catgaggccg ccgaccggca
     7741 gcgcgacctc ggcgagggcc tgggctccgt ccgcgacgac gatcccgccg ccgatcgcgg
     7801 cgacgcgccg gacggccgtc tccatggacg cgtcgtccgc gccgacgacg acgacgttgt
     7861 gcgagtcgtg cgcgacggac gacgcgacgg cgccgcgctt caggccgaag ccccgcgcga
     7921 acgcgacgcc cacgttcccg gtcccgcggt gccgctcgac gacggccagc ttcaggaggt
     7981 cgcgcgaaac gtccggcacg gcggcgccct ccgcgagggc cgcgcgctcg ttcgacgcgc
     8041 ccgtcgcgat cgagcccgcg cgcacctcga tgacgcgcag cgaggcgcct tcccccgccg
     8101 cgaccctgag ccgcgtgcgg tcgaagcctt ccacgcggaa gccgggccct tcctccatgc
     8161 gcgcggccgg gacgtccacg agaagctgtc cgcccttcgc gacgcgccgc ccgccgagga
     8221 ccacgaggtc cggtcggaag tcgtcgaggc gctcgaacgt gaggaggtcc gcgcggaagc
     8281 cgggcgcgac ggcgccgcgg tccgtgaagc cgtagtgccg ggcgcaggac cacgacgcga
     8341 ggcgcagcgc gagaacggga tccagcccga gcgcgatcgc gcgcctcacg tggtggtcga
     8401 ggtggccgtg ccgcagcagg tcccacgggt gccggtcgtc cgtcgcgaac gcgaagcgct
     8461 cggagttcgc ggccgtgacg agcggcagca actcgtcgag gttcttcgcg gcggtgccct
     8521 cgcggatcca gatgtccatt ccccggcgca ccttctcgtg cgcctcggcg cgcgagaggc
     8581 actcgtggtc cgagcggatg ccggcgccga cgtacgcgtt caggtcgagg ccggcgagaa
     8641 gcggcgaatg gccgtcgatc gggacgcgcc tcctccgcgc gtcctccacc ttcgccagga
     8701 cgcccgggtc cccgaaaaga aagcccggga cgttcatgag ctcggcgagg ccgagcacgt
     8761 tcgggaggtt gtagaggggc gcgaggtcgt ccgccgagag cgcggcaccc gccgtctcga
     8821 ggtgcgtcga ggggacgcac gacgggagca tgacgaagac gtccaccggg agccccgccg
     8881 ccgccgccag gaagtcccgg atgcccgcga cgccccgcac gttcgcgatc tcgtgcgggt
     8941 ccgtcacggc cgccgcggtc ccgtggacgg ccgcatgacg cgcgaacgtg cccggcgtcg
     9001 tcatcgtgga ctcgacgtgg aggtgcgcgt cccagagcgc gggggcgacg taggcgcccc
     9061 ggaggtcgac ggtgtcctct tcgcgctccg tgcggtagtc gccgaggccc aggatgcggt
     9121 cccccttcac gccgaccgtc ccgtcgtgga tctcgcccgt cagcacgttc acgacgcggg
     9181 cgttccggag gacgaggtcg gccgggacga cgccccgggc cgccgcgacg aagtcgcaaa
     9241 ggggcagggc ctcggggaga tcgctcacgc gcccgagagc gtacgacaga cgggcccgcg
     9301 tcgcgataga ctggaggaaa cggaggccgg actgagtacg tcgtttccga tccgcgaccc
     9361 gcagtcgggg gcttacacgc gcgcgttctt cgacgagatc gtcgaccggg aagtcgaacg
     9421 ctcgcgccgg cacggttcgg tcctctccgt gctgtcgatc gtcctcgcga actggaacga
     9481 cgtcacgtgc gcggcgcccg tcccggtcgt ggacgacgcg gtccggaccg tcgcgaacac
     9541 cctgctcgcg aacctcagga tgacggacta cctcttccgc tgggaggacg acgagttcct
     9601 cgcgctcctc gtcgaggcga acctcgacgg ctgcaagctg aaggtcgc
//
LOCUS       DAOMFY010000136         9531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2185_length_9531_cov_5.995568, whole genome shotgun sequence.
ACCESSION   DAOMFY010000136 DAOMFY010000000
VERSION     DAOMFY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2185_length_9531_cov_5.995568"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLB01_11635"
     CDS             <1..729
                     /locus_tag="PLB01_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77996.1"
                     /translation="ALVLVAAPEPSPTPTPTATATPTRTPEIRNPRLVIALESWQGVG
                     RERTALFDDGTLVKVLSFRGVDTMERRTVSPQELEVIKRVCQDALVVERRDVEDPSRM
                     VLGDTAARRMRLEIADGPGKIRAYPFDELSTLPLAVGHARGAIEDLRARFEKEDPSEV
                     NWDTRGLKAGSLLKKRSDGVWYRVVHDDALDHDFELEELAVSGGDGSHKMRLFLYRGD
                     IARLFENPEKAGPAPTPVPPARRH"
     gene            730..1350
                     /locus_tag="PLB01_11640"
     CDS             730..1350
                     /locus_tag="PLB01_11640"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HQQ77997.1"
                     /translation="MIRITGGEWRGRKLEVPGGIRPTTELARKAAFDILGEAIRGARV
                     LDACAGSGAYGLEALSRGAAHATFVESDRKVAEVLRGNVERLGALRRSSVEVMSVDRF
                     SSKVNMAAARADAPRADGRRGEPASHSPSNRLPSEAIARWDVIFHDPPFKEISSESDL
                     QLLFPLVRPGGWLFHERGDDLVVSPGGVAPSDRRRYGLTRFLIYRP"
     gene            1407..2573
                     /gene="metK"
                     /locus_tag="PLB01_11645"
     CDS             1407..2573
                     /gene="metK"
                     /locus_tag="PLB01_11645"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQQ77998.1"
                     /translation="MSKNRHLFTSESVTEGHPDKIADQISDAILDAILTDDPMGRVAC
                     ETLVTTGLALIAGEITTKTYVDIPGIVRQTIKDVGYTRAKYGFDYETCAVISTIDKQS
                     PDIAMGVDTGGAGDQGLMFGLAVRETDELMPLPITLAHKLTMRLAEARRNRTLEWLRP
                     DGKSQVSVEYADGKPVRVDTVVISTQHSESIPTPELREAIREEIIKPVVPAALMDEKT
                     KFHINPTGRFVTGGPQGDTGLTGRKIIVDTYGGYGRHGGGAFSGKDATKVDRSASYMA
                     RYIAKNLVAAGLADKVEVQLAYAIGVADPVSVYVDAFGTSKVDPEMLSKIVREHFLLT
                     PKGIIETLQLRRPIFKKTASYGHFGRRLPEFTWEQTDKAAALASAAGAAMSAKA"
     gene            2688..3110
                     /locus_tag="PLB01_11650"
     CDS             2688..3110
                     /locus_tag="PLB01_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ77999.1"
                     /translation="MDANVLPQGADPLEKAATERREFLAAAARFAGVLAALGITGDAW
                     TAAAAEQKVAPGAAVQHKPFLQGTERTRFIRSVLEDAIKSGDAQGALRKAGADGKLAP
                     ADISKISSLTGAELQAINSVKLKIPDLGAGADTGGIIY"
     gene            3171..4256
                     /locus_tag="PLB01_11655"
     CDS             3171..4256
                     /locus_tag="PLB01_11655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ78000.1"
                     /translation="MPDRKRFAVSQRGAGRPLAVAKRFHRTVGVNLTMRCPLECAHCS
                     VDSGPWRKETVDGNLLASRIEEMGRDGRIRLVMVSGGEPFSARDALRKVLEAAEPWGI
                     SVFVLTSGIWAKGREAADRTLDAFPSLRYVGFSLDEYHEPFVPMEDVKSGVLAALARG
                     LLVDLTIRVWDPEGDPYLDRIAETFGDELLSRVGIDLERIVPVGRARGLPQAGVEELP
                     GLPAGACDSAHQPVIDTDGTVVGCCNVALARRTPPLVLGSLRDVGLPALTRAAESDVL
                     RHAIRVFGPKYLADLVVSRGEGGRLAARYPGDICALCHDVLRQPELVAILNEELSRPE
                     RRAEIALGRLLRFGEEDPALVPAGAAS"
     gene            4253..5305
                     /locus_tag="PLB01_11660"
     CDS             4253..5305
                     /locus_tag="PLB01_11660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ78001.1"
                     /translation="MSAEAAPGRDTAFAQTIVFVPTLRCPLRCLHCVTESGPDVRREM
                     KPEDAERWIDGLAATGRVRRLVLSGGEPFVDLARLDAILEAASGRDLAVSAVTSASWA
                     PTPERARRVLESRPALSEICVSVDRWHLEFLSLGNARHAIEAAVALGRRSTVFATEAP
                     ADGSVVASLRASLDPAVASRLEIRREPLHFAGRARGAGASALAPHIAWGPLEAAVEGP
                     CTGPAAPAVLPDGTVLACCGDAISAPEEWTALVLGSLATESAESILARADANPLLHAI
                     RLLGPKALLLKARELGAASGGREYERDNICDTCRDLAAGGATRARLRPWLEAETGSLG
                     LARAAVLGEGWPEGDP"
     gene            5302..5979
                     /locus_tag="PLB01_11665"
     CDS             5302..5979
                     /locus_tag="PLB01_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HQQ78002.1"
                     /translation="MSVTPEPGLGARLLAVRAEFDDVYRFGFAKLLSATGGRARLVHY
                     LTETWHYVRFSVPLLERAAARIDDDLHDLRAWFERHVAEEKGHDDWLLDDLERLGVSR
                     VTVRTSLPSRETATLVGTQLWVLEALHPAALLGYVWSLESRPPGTAGLDALAAAFDAP
                     PEALSTLRAHGTMDPGHLAQILEVADGIPLGPVADRILWNARIASAQAADVVLGAAVR
                     NLDIMPF"
     gene            6000..6965
                     /locus_tag="PLB01_11670"
     CDS             6000..6965
                     /locus_tag="PLB01_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ78003.1"
                     /translation="MLDAAPALAALADPLRLRTVRLLAKEELQVREIQRILEAAQSRV
                     SNHLAVLRHAGLVETRRTNGSERYAATAEGTKLWESVSGAVSDAAFPTDDDRLKDVLE
                     ERTAVSPEGGFDAVAGEWDRIQGPFYASGAREAALLKLVPRGLRVADVGCGTGLLTLG
                     LASVADEVHAVDSSDRMVRLAREKVRRAGLAHVTVRKASAEDLPFADASLDAVFAFHL
                     LRHLARPAEALAEFGRVVKPGGRVVLVELEPHGHRALREATGSTHLGLPRETVRAGLK
                     RAGFADARFSALDPYRVPSEAGETLLPTYLVDAVRAARGEQKEPS"
     gene            6962..8305
                     /gene="ahcY"
                     /locus_tag="PLB01_11675"
     CDS             6962..8305
                     /gene="ahcY"
                     /locus_tag="PLB01_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQQ78004.1"
                     /translation="MTTATLPAHDVKDLALAAKGRLRMEWADRSMPVLARIRERFEKE
                     KPLKGMRLSACLHVTSETGNLMRALKAGGADVVLCGSNPLSTQDDVAAALVAEFGIST
                     YAIKGEDHKTYYAHIQAALAHRPNVTMDDGADLVSSLLFMALGKEGQLEPSLAAWAKG
                     LSASDKKSLLDGIVGGTEETTTGVIRLKAMEKEGVLKFPVVAVNDAETKHFFDNRYGT
                     GQSTLDGIIRATNLLVAGLNVVVAGYGWCGKGVAMRAKGLGANVIVTEINPVRGIEAA
                     MDGFRVMPMAEAAKVGDIFVTVTGNKNVLADEHFAVMKSGAVISNSGHFNVEIDIPAL
                     EKRAGSRRMIREFVEEFRLKDGRIVFLLGDGRLINLAAAEGHPASVMDMSFANQALGA
                     EYMVRNHATLEKKVYSIPQAIDAEIARLKLASMGVALDVLTPEQEKYLAGWEEGT"
     gene            complement(8413..>9531)
                     /locus_tag="PLB01_11680"
     CDS             complement(8413..>9531)
                     /locus_tag="PLB01_11680"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQQ78005.1"
                     /translation="RGAASRRTMRLPGLLLIDRYVARRFLATFLLVVASIAVLYVLID
                     YLEISDDIARNKTPFAMVTRYYQAFLAPILLDIVPFAFLVAALVTTAGLVRSGETTAL
                     LSHGVSLHRAAAPLLVMAAAAGVFLFLFAERVVPAAAAESDRLRYLLKGQTPPAAGAT
                     GVWVRGEHGRFFASAAYDAATRRVDGLTMLQIDPKSWHLEARTDAEHATVVPGRGLLA
                     RGGWVRSFTGPSAMAARRDDDFILEAPEAAAAFVAGPSDIKRMKFFELRRYIAARRRA
                     GADAAALATGLYQKTSTAAAALLLTLVGLPFAFKYGKRGAVAGIGVALFLGLTYLFLT
                     SLFVRFGESGSLPPLLAAWVPNVFFGLGAAWGLLGVRT"
BASE COUNT         1457 a         3374 c         3337 g         1363 t
ORIGIN      
        1 gcgctcgtgc tcgtcgcggc gcccgagccg tcgcccacgc cgacgccgac cgccacggcg
       61 acgccgaccc ggacccccga gatccgcaat ccccgcctcg tcattgcgct ggagtcctgg
      121 cagggcgtcg gccgcgagcg gacggcgctc ttcgacgacg gcactctcgt gaaggtcctg
      181 agcttccgcg gagtcgacac gatggagcgg cggaccgtct cgccgcagga gctcgaggtc
      241 atcaagcggg tctgccagga cgcgctcgtg gtcgagcggc gcgacgtcga ggacccatcc
      301 cgcatggtgc tgggagacac ggccgcccgg cgcatgcggc tggagatcgc ggacggaccg
      361 ggaaagatcc gcgcctaccc gttcgacgag ctctcgaccc tcccgctcgc cgtcgggcac
      421 gcgcggggcg cgatcgagga ccttcgcgcg cgtttcgaga aggaggaccc gtccgaggtc
      481 aactgggaca cgaggggcct caaggccggc tcgcttctca agaagaggtc cgacggcgtg
      541 tggtaccgcg tcgtccacga cgacgcgctc gaccacgact tcgagctcga ggagctggcc
      601 gtctcggggg gcgacggcag ccacaagatg cgcctctttc tctaccgcgg ggacatcgcc
      661 cggctcttcg agaatcccga gaaggcgggg ccggccccga cgccggtccc ccccgcaagg
      721 cggcactaga tgatccgcat cacgggaggg gagtggcgcg ggcgaaagct cgaggtgccc
      781 ggcgggatcc ggccgacgac ggagctcgca cgcaaggcgg cgttcgacat cctcggcgag
      841 gcgatccgcg gcgcgcgcgt cctcgacgcg tgcgcgggct cgggcgccta cggtctcgag
      901 gccctttcac gcggggcggc gcacgcgacg ttcgtcgagt cggaccggaa ggtcgcggag
      961 gtcttgaggg ggaacgtgga gcggctcggc gcgctgcgac gctcgagcgt agaagtcatg
     1021 tccgtggatc gtttttcttc gaaggtgaac atggccgcgg cgcgagccga cgctcctcgc
     1081 gcggacggcc gccgcggcga gcccgcatcc cattcacctt caaaccgtct cccgagcgaa
     1141 gccatcgcgc gatgggacgt gatcttccac gatccccctt tcaaagaaat ctcctccgaa
     1201 tccgatcttc aactcctgtt tcctctcgtg cgccccggcg gctggctgtt ccacgagcgc
     1261 ggcgatgacc tcgtcgtttc ccccggcggc gtcgcgccct ccgaccggcg ccgttacggc
     1321 ctcacgcggt tcctcatcta ccgtccctga cgggtttccc gcctgctaac atccgccctt
     1381 cgccgcacgc ggcgcggagt catttcatga gcaagaaccg gcacctcttc acgtccgagt
     1441 ccgtcaccga ggggcatccc gacaagatcg ccgaccagat ctcggacgcc atcctcgacg
     1501 ccatcctgac ggacgacccg atgggccgcg tcgcgtgcga gacgctcgtc acgacgggcc
     1561 tcgccctcat cgcgggcgag atcacgacga agacgtacgt cgacatcccg ggaatcgtgc
     1621 gccagacgat caaggacgtc ggctacacgc gggcgaagta cgggttcgac tacgagacgt
     1681 gcgccgtgat ctccacgatc gacaagcagt cgccggacat cgcgatgggc gtcgacaccg
     1741 gcggcgccgg cgaccagggg ctcatgttcg gcctcgccgt tcgcgagacc gacgagctca
     1801 tgccgctccc gatcacgctc gcgcacaagc tcacgatgcg cctcgccgag gcgcgccgga
     1861 accggacgct cgagtggctg cgcccggacg gcaagagcca ggtttccgtg gagtatgcgg
     1921 acggcaagcc cgtgcgcgtg gacacggtcg tcatctcgac gcagcactcc gagtcgatcc
     1981 cgacgcccga gctccgcgag gcgatccgag aggaaatcat caagcccgtc gtccccgccg
     2041 ccctcatgga cgagaagacg aagttccaca tcaacccgac cgggcgcttc gtgacgggcg
     2101 ggccgcaggg cgacacggga ctcacgggcc gcaagatcat cgtcgacacg tacggcggct
     2161 acggccggca cggcggcggc gcgttctccg gcaaggacgc gacgaaggtc gaccgctcgg
     2221 cgtcgtacat ggcgcgctac atcgcgaaga acctcgtcgc cgccggtctc gccgacaagg
     2281 tcgaggtcca gctcgcatat gccatcggcg tcgcggaccc ggtgtccgtc tacgtggacg
     2341 cgttcgggac ctcgaaggtc gaccccgaga tgctctcgaa gatcgtccgg gagcacttcc
     2401 ttctcacccc gaagggaatc atcgagacgc tccagctgcg ccggccgatc ttcaagaaga
     2461 ccgcgtccta cggccacttc ggccgcaggc tccccgagtt cacgtgggag cagacggaca
     2521 aggccgcggc gctcgcgtcc gcggcgggcg cggcgatgtc ggcgaaggcc tgacccggct
     2581 gtcccctccc ggacctgttt gacgattttt ctgttgcccg ggaatccgct ggcatcggag
     2641 aattcgtcgc aatctcagga ccgaaaaacg aaggaggaga aagtcgaatg gacgcaaacg
     2701 ttcttcccca gggggccgat ccgctcgaga aagccgccac ggagagacgc gagttcctcg
     2761 cagccgcagc ccgtttcgcg ggcgtcctcg ctgcactcgg gataacgggt gacgcctgga
     2821 cggccgcagc cgccgagcag aaggtcgctc ccggggcggc cgtgcagcac aagcccttct
     2881 tgcagggcac ggagcgcacg cgattcatcc gctcggtcct cgaggacgcg atcaagagcg
     2941 gcgacgctca gggcgcgctc cggaaggctg gagccgacgg aaagctcgcc cccgcggaca
     3001 tctcgaagat ctcgtcgctc acgggcgccg agctgcaggc gatcaactcc gtgaagctga
     3061 agatccctga tctcggcgcc ggggcggata cgggcggcat catctactag cggatctctc
     3121 gtcgcgggcg gctccggcgc gtgcggggcc gcccgccgag cccgcgagcc atgccagaca
     3181 ggaagcggtt cgccgtgtcg caacgcggcg ccggtcgtcc cctcgccgtc gccaagaggt
     3241 tccaccgcac cgttggcgtc aacctcacga tgcggtgccc gctcgagtgc gcgcactgct
     3301 ccgtggacag cggcccgtgg cggaaggaga cggtggacgg gaacctcctc gcctcgcgca
     3361 tcgaggagat ggggcgggac ggtcgcatcc ggctcgtgat ggtctcgggt ggggagccgt
     3421 tcagcgcgcg cgacgcgctc cggaaggtgc tcgaggcggc cgagccgtgg gggatctcgg
     3481 tgttcgttct cacgagcggg atctgggcaa agggccgcga ggccgcggac cggaccctgg
     3541 acgcgttccc gtcgcttcgc tacgtagggt tcagcctcga cgagtaccac gagccgttcg
     3601 tcccgatgga ggacgtgaag tcgggggtcc tcgcggccct cgcgaggggc ctcctcgtgg
     3661 acctcacgat ccgcgtctgg gatcccgagg gcgatccgta cctcgaccgg atcgcggaga
     3721 cgtttggcga cgagctcctc tcccgggtgg ggatcgacct cgagcggatc gtcccggtgg
     3781 gccgggcgcg cggcttgcca caggctggag tcgaggagct cccggggctc cccgccgggg
     3841 cctgcgacag cgcccaccag ccggtgatcg acacggacgg gaccgtcgtc ggctgctgca
     3901 acgtcgcgct cgcgcgccgt acgccgccgc tcgtcctggg ctctctccgc gacgtcgggc
     3961 ttccggcgct cacgcgcgcc gcggagagcg acgtccttcg ccatgcgatc cgcgtcttcg
     4021 gcccgaagta cctcgcggac ctcgtcgtct cacggggcga gggcggaagg ctcgcggcgc
     4081 gctatccggg ggacatctgt gcgctctgcc acgacgtctt gaggcagccg gaactcgtcg
     4141 cgatcctgaa cgaggagctt tcacggcccg agcggcgcgc ggaaatcgcg ctcggacggc
     4201 tcctgaggtt tggagaggag gatcccgcgc tcgtccccgc cggggcggcg tcgtgagcgc
     4261 cgaggccgcc ccgggccgcg acacggcgtt cgcgcaaacg atcgtcttcg tcccgacgct
     4321 ccgctgcccc cttcggtgcc tgcattgcgt gacggagtcc ggccccgacg tgcggcgcga
     4381 aatgaagccc gaggatgcgg agcggtggat cgacgggctc gccgcgaccg gccgcgtccg
     4441 gcggctcgtg ctctcgggag gggagccgtt cgtcgacctc gcgcgcctcg acgcgattct
     4501 cgaggccgcg tccggccggg acctcgcggt ctccgccgtg acgagcgcgt cgtgggcgcc
     4561 gacgcccgag cgggcgcggc gcgtcctcga gtcgaggccc gcgctctccg agatctgtgt
     4621 gagcgtggac cgctggcacc tcgagttcct gtcgctcggg aacgcacggc acgccatcga
     4681 ggccgcggtc gcactcgggc ggcggtcgac cgtcttcgcg acggaggcgc cggccgacgg
     4741 gtcggtcgtc gcgagcctcc gagcgtcgct cgatcctgcg gtggcctccc gcctggagat
     4801 tcggcgggag cctcttcact tcgcgggccg ggcgcgcgga gcgggcgcgt cggcgctcgc
     4861 gccgcacatc gcgtggggcc cgctcgaagc cgctgtcgaa ggtccgtgca cgggtccggc
     4921 ggcgcccgca gtgctcccgg acggaacggt cctcgcgtgc tgcggcgacg cgatctcggc
     4981 gccggaggaa tggacggcgc tcgtcctcgg ctcgctggcc acggagagcg ccgagtcgat
     5041 tctcgcccgc gcggacgcaa accctcttct ccacgcgatt cgtctcctcg ggccgaaagc
     5101 actgctcctc aaggcgcggg aactcggcgc cgccagcggc gggcgtgagt acgagcgcga
     5161 caacatctgc gacacctgcc gggacctggc cgcgggcggc gcgacgcgcg ctcgtctccg
     5221 tccgtggctc gaagccgaga ccggaagcct cggcctggcg cgcgcggcgg tcctcggtga
     5281 gggctggccg gagggcgatc catgagtgtg acgccggaac ccggcctcgg cgcgcgcctc
     5341 ctcgccgtgc gcgccgagtt cgacgacgtc taccgcttcg gcttcgcgaa actcctgtcg
     5401 gcgacgggcg gacgcgcccg gctcgtccac tacctcacgg aaacgtggca ctacgtccgg
     5461 ttctccgtgc ccctcctcga gcgcgccgcc gctcggattg acgacgacct gcacgacctg
     5521 cgcgcctggt tcgaacgcca cgtcgcggaa gagaaaggtc acgacgactg gctcctcgac
     5581 gacctcgagc gtctcggggt ctcgcgcgtg accgttcgca cctccctccc gtcgcgcgag
     5641 acggcgacgc tcgtgggtac gcagctctgg gtcctggagg cgctgcaccc ggcggcgctc
     5701 ctcggctacg tgtggtctct cgagtcccgg ccgcccggca ccgccggcct cgatgcgctc
     5761 gccgcggcgt tcgacgcacc gccggaggcg ctttcgacgc tgcgcgcgca cggcacgatg
     5821 gatccgggtc atctcgcgca gatcttggag gtcgccgacg gcattccttt gggaccggtc
     5881 gccgatcgga tcctgtggaa cgcccgcatc gcctctgccc aggccgccga cgtcgtgctc
     5941 ggggccgccg tccgaaatct cgatatcatg ccgttctgat tttgtgatat aaagcccggg
     6001 tgctggacgc cgcccccgcc ctcgccgcgc tcgcggaccc cctccgcctg aggaccgtga
     6061 ggctcctcgc gaaggaggaa ctccaggtcc gcgagatcca gcggatcctc gaagcggccc
     6121 agtcgcgcgt ctcgaaccac cttgccgtgc tgcgtcatgc cggcctcgta gagacgcgcc
     6181 ggacgaacgg gtcggagcgc tatgcggcga ccgccgaggg cacgaaactc tgggagtccg
     6241 tctccggggc ggtctcggac gcggcgttcc ccacggacga cgaccgtctc aaggacgtcc
     6301 tcgaggagcg caccgccgtg tcgcccgagg gcggcttcga cgccgtcgcg ggcgagtggg
     6361 accggatcca ggggccgttc tacgcgtccg gcgcccgcga ggccgcgctc ctgaagctcg
     6421 tcccgcgcgg ccttcgcgtc gccgacgtcg ggtgcggcac ggggctcctg acgctcggcc
     6481 tcgcgtccgt cgccgacgag gtgcacgcgg tggattccag cgatcgcatg gtccggctcg
     6541 cccgcgaaaa ggtccggcgc gccggcctcg cgcacgtgac ggtccggaag gcctctgccg
     6601 aggacctgcc gttcgcggac gcctcgctcg acgccgtctt cgcgttccat ctcctgcgcc
     6661 acctcgcgag gcccgccgag gccctcgccg agttcggccg cgtcgtgaag ccgggcggcc
     6721 gggtcgtcct cgtggagctc gagccgcacg gccaccgggc cctgcgcgaa gccacgggct
     6781 cgacccatct gggcctcccg cgcgagacgg tgcgcgcggg cctcaagcgc gccggctttg
     6841 ccgacgcccg cttctcggcc ctcgaccctt accgcgtccc ctccgaagcg ggggagacgc
     6901 tgcttcccac gtatctcgtc gacgccgtgc gcgccgcgcg cggcgaacaa aaggagccct
     6961 catgacgacc gccaccctcc ccgcccacga cgtcaaggac ctcgcgctcg ccgcgaaggg
     7021 ccgtcttcgc atggaatggg ccgaccgctc catgcccgtc ctcgcccgca tccgggagcg
     7081 cttcgagaag gagaagcccc tgaagggaat gcgcctctcg gcctgtctcc acgtgacgag
     7141 cgagacgggc aacctcatgc gcgccctcaa ggcgggcggc gcggacgtcg tgctctgcgg
     7201 gtccaacccg ctctcgacgc aggacgacgt cgcggcggcg ctcgttgccg agttcgggat
     7261 ctcgacctac gcgatcaagg gcgaggacca caagacgtac tacgcccaca tccaggcggc
     7321 gctcgcgcat cgccccaacg tcacgatgga cgacggagcc gacctcgtgt cgtcgctcct
     7381 cttcatggcg ctcggcaagg aagggcagct cgagccctcc ctcgccgcgt gggcgaaggg
     7441 tctgtccgcg tccgacaaga agtcccttct cgacgggatc gtcggcggga cggaggagac
     7501 gacgaccgga gtcatccgcc tcaaggccat ggagaaggaa ggcgtcctga agttccccgt
     7561 cgtcgccgtc aacgacgccg agacgaagca cttcttcgac aaccgctacg gcacgggcca
     7621 gtccacgctc gacgggatca tccgcgcgac gaacctgctc gtcgcgggcc tgaacgtcgt
     7681 cgtcgccggc tacggctggt gcggcaaggg cgttgcgatg cgcgccaagg gcctcggcgc
     7741 gaacgtcatc gtgaccgaga tcaacccggt ccgcggaatc gaggccgcga tggacggctt
     7801 ccgcgtgatg ccgatggccg aggccgccaa ggtcggcgac atcttcgtca cggtcacggg
     7861 caacaagaac gtcctcgccg acgagcactt cgccgtcatg aagtcgggcg ccgtcatctc
     7921 gaactcgggc cacttcaacg tcgagatcga catcccggcg ctcgagaagc gggccggctc
     7981 gagacgcatg atccgggagt tcgtcgagga gttccggctg aaggacgggc gaatcgtgtt
     8041 cctcctgggc gacggccgcc tcatcaacct cgcggccgcc gagggccacc cggcgtcggt
     8101 gatggacatg tccttcgcga accaggcact cggggccgag tacatggtca ggaaccacgc
     8161 gacgctcgag aagaaggtct actcgatccc gcaggcgatc gacgccgaga tcgcgcgcct
     8221 caagctcgcg tccatgggcg tcgcgctgga cgtcctcaca ccggagcagg agaagtatct
     8281 ggcgggttgg gaagagggaa cgtaagagaa aacggagatt tcttagaagg gtaatagggc
     8341 ttgggaaggc ggccgacgcg gcgtggcgcc ggccacgcac cccgctcggg caccttctaa
     8401 gaagatcttc tcttaggttc ttactccgag cagcccccag gccgcgccga gaccgaagaa
     8461 gacgttcggc acccaggcgg cgaggagggg agggagagag cccgactccc cgaaccgcac
     8521 gaagagggac gtcaggaaca ggtaggtcag cccgaggaac agcgcgacgc cgatccccgc
     8581 gacggcgccg cgctttccgt atttgaaggc gaaggggagc ccgacgagcg tgagcaggag
     8641 cgccgccgcc gccgtcgacg tcttctggta gaggcccgtc gcgagcgccg ccgcgtcggc
     8701 gcccgcgcgc cgccgcgccg cgatgtaacg gcgcaactcg aagaacttca tgcgcttgat
     8761 gtcggacggc cctgcgacga acgcggccgc ggcctcgggc gcctcgagga tgaagtcgtc
     8821 gtcccgccga gcggccatgg cggaggggcc cgtgaacgac cgcacccagc cgccccgcgc
     8881 gagaagcccg cgtccgggga cgaccgtcgc gtgctccgcg tccgtccgcg cttcgaggtg
     8941 ccagctcttc ggatcgatct gcagcatcgt gaggccgtcc acgcgccgcg tcgcggcgtc
     9001 gtaggcggcc gacgcgaaga agcgcccatg ctcgccgcgc acccacacgc cggtggcgcc
     9061 cgcggccggg ggcgtctggc ccttgaggag gtagcgcagg cggtcggact ccgccgccgc
     9121 ggcgggtacg acgcgctcgg cgaagagaaa cagaaagacg cctgccgctg cggccatgac
     9181 gaggagcggc gcggcggcgc ggtgcagcga gacgccgtgc gacaggaggg ccgtcgtctc
     9241 tccggagcgt acgagacccg cggtcgtgac gagggccgcg acgaggaacg cgaagggcac
     9301 gatgtcgagg aggatggggg cgaggaacgc ctggtagtag cgcgtcacca tcgcgaacgg
     9361 cgtcttgttc cgcgcgatgt cgtcggagat ctcgaggtag tcgatcagga cgtagagcac
     9421 cgcgatggac gcgacgacga gcaggaacgt ggcgaggaac cggcgcgcga cgtagcggtc
     9481 gatgaggagg aggccgggca gacgcatcgt ccgccggctc gcggcgcccc g
//
LOCUS       DAOMFY010000137         9527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2187_length_9527_cov_8.013514, whole genome shotgun sequence.
ACCESSION   DAOMFY010000137 DAOMFY010000000
VERSION     DAOMFY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2187_length_9527_cov_8.013514"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLB01_11685"
     CDS             complement(<1..509)
                     /locus_tag="PLB01_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78006.1"
                     /translation="MSRRLAACVAAAFLLLAATAEERTFGTVTDEQQMLYTAISIATT
                     GEIGISRNQRFSVSRPAGDAVSPYGMGLPLLEAPLAALAGPWEARFGVRTSQTLFVLL
                     QVLLVTAAAAGAGLAAGAFGAGAFGQALAVFAAALASPLWAYTATGYSEPLQAVCLVF
                     AFVLASAASR"
     gene            complement(506..1105)
                     /locus_tag="PLB01_11690"
     CDS             complement(506..1105)
                     /locus_tag="PLB01_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQQ78007.1"
                     /translation="MAPPFDRPMLAVFCGARAGVSTAYADEASAVGREIVRAGAGLVF
                     GGGRVGLMGVLADAVLEEGGIAYGVIPAGLFAKEVAHGGLTRLEVVNTMHERKALMAD
                     MADGFLALPGGYGTLDEFFEIVTWRQLGHHARPIVLYNRSGFFDPLLAATRRIADEGF
                     GREGGAYFAVETDPAVAVRRALAGAPRPALPVHGGEDRR"
     gene            1152..1745
                     /locus_tag="PLB01_11695"
     CDS             1152..1745
                     /locus_tag="PLB01_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQQ78008.1"
                     /translation="MESSDRLDPARAALRTALLRHRPADDREAGDRAAILALVENEPG
                     CFARTTFAPGHVTGSGFVVHAATRRVLLHHHRRLDAWLQMGGHDEGESDPRATALRES
                     AEESGLTDLVLLSPEILDVDIHPIPAGKGEPPHLHHDVRYALATRAPDAIRRDDAESI
                     ALRWFTLDEAAAAMADPDAARALDRIARLLDGVSSGR"
     gene            complement(1752..3599)
                     /locus_tag="PLB01_11700"
     CDS             complement(1752..3599)
                     /locus_tag="PLB01_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQQ78009.1"
                     /translation="MIEALPAQTLVDYFRHAAASGKAELLVSKVDGAWKPVSAGEFEE
                     RTKGLALGLALLGVDREDRVAILSENRPEWPMTDFATLSLGALTVPIYTSYLAPQVEY
                     ILRDSLAKVVVVSNAIELQKVLDVRDRCPELRHVVVLEQVPWSAGRAVPFETVVQKGL
                     SALAADPEAWEERASSLLPTDLATMIYTSGTTGEPKGAILTHGNFVANVATCSTLFDV
                     TPDMVALSFLPLSHVFERMVDYLFFSRATTIAYAESIDKLSENFAEIRPHCFAAVPRV
                     YEKMLARVRNAVDAAPALRRAIFEWGVRIGAMHLRAVESGEKPPFFLRLKYRIADRLV
                     FGKIKARLGGRFLFAISGGAPLAHDVAAFFWAAGVEVYEGYGLTETSPVLSCNRPGEW
                     RLGTVGRAIPGVTLKIAPDGEVLAKGPNVMDGGYWRKKEETASVFDADGWFLTGDIGS
                     FDLDGFLTLTDRKKEILINAYGKNIAPAPIEAALKAVRFVSSAVLIGDRQKFLSALIV
                     PNFEKLEGWAAGANVSYRTRQELVEEPKVLALFKQAIEILNGDEPHERQIRAFRLLTE
                     DFSIEGGELTPTMKIKRRVVASKYRDVIEEMYADAAAFEDRNADALEGR"
     gene            complement(3643..4848)
                     /locus_tag="PLB01_11705"
     CDS             complement(3643..4848)
                     /locus_tag="PLB01_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ78010.1"
                     /translation="MKVLVADKFEESGLDGLKAIGCEVLYEPKLEGETLGARLAESRA
                     DVLVVRSTKVTAAEIDRASGLSLIVRAGAGVNTIDLAAASARAVAVSNCPGKNAIAVA
                     ELAWGLLLALDRRLVDQAVDLRNGVWNKAEYGKAKGLAGRTLGVIGLGAIGLEVVTRA
                     QAFGMPVVAWSRSLDDDHAERLGVSRADSIHALAAASDAVSVHCALTKETKGFLDAKF
                     FEALKPGAFFVNTSRGEIMDAAALRRAISERGVRAGLDVFEKEPAGGSGPFEDDIVRL
                     PGVVGSHHVGASTDQAQNAIAAETVRIVAAFAASGAIPNAVNLASRTPATHLLTVRHR
                     DRVGVLAHVFGVLKAAGINVQQTENVVFAGAEAACARIQVDQGPGPAVLDEIRSGCAD
                     VLSVGLTVL"
     gene            complement(4869..6095)
                     /locus_tag="PLB01_11710"
     CDS             complement(4869..6095)
                     /locus_tag="PLB01_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HQQ78011.1"
                     /translation="MARIRPFRSGRPPKALAARVASVPYDVVDTKEARALAEGNPVSF
                     LHVCRPEIDLPEATDIHADEVYAKGRENLDGFYADGTLVEDPVPRLLIYRQTWRGRSQ
                     DGFVAACSVDDYDADVIRKHEKTRKDKEDDRVRHLLTQSAHAEPVFLTCRAVDAVDAR
                     VAAVTAQAPEFDFEAPDAVRHTLWLVPEADVAFFVSAFAAVPLLYVADGHHRCASASR
                     ARATLAAKGPLADDHPVNWFPAAIFPDRQLAILPYNRVVKDLGGMGKEDFLRRVRERF
                     DVQPAESPEPRRRGTFSLYLEENWQTLTPREPATGNDPVSRLDVSLLQDRLLKPVLGI
                     GDPRTDPRIDFVGGIRGTSELEERVRSGRGAVAFAMYPTQISDVMAIADAGVTMPPKS
                     TWFEPKLRSGLFVHRF"
     gene            6212..6484
                     /locus_tag="PLB01_11715"
     CDS             6212..6484
                     /locus_tag="PLB01_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQQ78012.1"
                     /translation="MKLYVGNLSYGVNDASLRALFEAYGAVESATVVADRDSGRSKGF
                     GFVEMSDADAQKAIQALNGSQHDGRTIRVNEARPKTDAPRGPRGPR"
     gene            6736..8211
                     /locus_tag="PLB01_11720"
     CDS             6736..8211
                     /locus_tag="PLB01_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ78013.1"
                     /translation="MDASGLYLAPGFIDLLGQSEYNVLVDPRAASKITQGITTEVTGE
                     GDAIAPNDEKMFREAEPTWKRYGVRADFFTLADYFARLAKAPPTINLGTFVGSGGLRQ
                     LVVGGENRRATPAEVARMAAEVEKAMQDGALGLSSSLQYVPDIYNSADEIVAMARAAA
                     KHGGVYFVHQRSESNAIDASLDEVFRIAREAKIPANVWHLKTAYKRNWGRMPAVLARL
                     EAARAEGLDVAANQYPWTAASNPLSSCLPPWMREGGNEKLLARLKDPALREKAKADMG
                     RDADDWQNQYFGSGGPSRIIVSAVLTPALKALEGRTIEEIAAARKKDARDALMDVVLE
                     DGGHSSCIFFCMDEEDVKTALKHRLVSFCTDSGASATDGIFSEEKSHPRGWASAARIL
                     GTYVRDEKVLPLEDAVRKMTSFAAARAGLKDRGLLREGFPADLVAFDLAKVRAVSTYA
                     DPLRYSEGFPYVAVNGVLVVDGSRITSERPGRALRGPGFAR"
     gene            complement(8219..9139)
                     /locus_tag="PLB01_11725"
     CDS             complement(8219..9139)
                     /locus_tag="PLB01_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HQQ78014.1"
                     /translation="MKILLADSFDESLPKRLAPFGEVLTDAARLKEADVVLVRSKTKV
                     TRDYLANAPNLKLVIRGGVGLDNVDIQACRERGIDVKNTPRASSVAVAELTLALMLAV
                     PSRVVEGHVGLKGGRFLKKELKRTELFGKTLGLVGAGLIGTEVARRAQAFGMKVLAYD
                     PQIANHALAELVDDLDELFRRADFISFHVPATAETRGLVNEHTIAKMKDGVILVNTAR
                     GSIVVEADLAAALASGKVRAYATDVYLSDPPDPNSPLLAAPNVLMTPHLGGSSQENLL
                     RIGDIVVDLMKKWQRVPLPATGAAAPVPAR"
     gene            complement(9262..>9527)
                     /locus_tag="PLB01_11730"
     CDS             complement(9262..>9527)
                     /locus_tag="PLB01_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78015.1"
                     /translation="GVTEGTPPPDAAASVSPWWLRRVLGLPAVPPMVPRLLVRSAAGY
                     LGRGETARSLPWLREAVQLSPRDPDAARLLAWAEGRGSPPATR"
BASE COUNT         1415 a         3365 c         3415 g         1332 t
ORIGIN      
        1 cgcgacgcgg cgctcgcgag gacgaacgcg aacacgaggc agacggcctg cagcggctcg
       61 gagtatccgg tcgccgtgta ggcccacagc ggcgacgcga gcgcggccgc gaagaccgcg
      121 agcgcctgtc cgaaggcccc cgcgccgaac gcgcccgccg cgagtcccgc gcccgccgcg
      181 gcagccgtca cgaggaggac ctgcaggagg acgaagagcg tctgggaggt gcggaccccg
      241 aaccgcgcct cccacggccc ggccagggcc gccagcggag cctcgagaag cgggagaccc
      301 attccgtacg gcgagacggc gtcgcctgcg gggcgggaga cgctgaagcg ctggttgcgc
      361 gagatcccga tctctcccgt cgtcgcgatc gagatcgccg tgtagagcat ctgctgctcg
      421 tcggtgacgg tcccgaacgt ccgttcctcg gccgtcgcgg cgagcaggag gaacgccgcc
      481 gcgacgcacg cggcgaggcg gcggctcacc ggcggtcctc tcccccgtgg accggcaagg
      541 cgggacgcgg cgccccggcg agggcgcgac gaaccgccac ggccgggtcg gtctcgacgg
      601 cgaagtaggc gccgccttca cgcccgaacc cctcgtccgc gatgcggcgc gtggccgcga
      661 gcagcgggtc gaagaagccg gagcgattgt agaggacgat cggccgcgcg tggtgcccga
      721 gctggcgcca ggtcacgatc tcgaagaact cgtcgagcgt gccgtatccg ccgggcaggg
      781 cgagaaagcc gtccgccatg tcggccatga gcgccttgcg ctcgtgcatc gtgttcacga
      841 cctcgagacg cgtgaggccg ccgtgcgcga cctctttcgc gaagaggccc gcgggaatca
      901 cgccgtacgc gatgccgccc tcctcgagca ccgcgtccgc gaggacgccc atgagcccca
      961 cgcgcccgcc cccgaagacg aggccggcgc ccgcgcggac gatctcgcgg cccaccgccg
     1021 aggcctcgtc ggcgtaggcc gtcgagacgc cggcgcgcgc cccgcagaac acggcgagca
     1081 tgggacgatc gaaaggcggg gccatctccc cggatgatgc ccgatcccgc gtcgcctgct
     1141 aaggtccgtc cgtggaatct tccgatcggc tcgaccccgc ccgggccgcc ctgcggaccg
     1201 ccctgctgcg ccatcgcccg gcggacgacc gcgaggcggg cgaccgcgcc gcgatcctcg
     1261 cgctcgtaga gaacgagccc ggctgcttcg cccggacgac tttcgcgccg gggcacgtca
     1321 cgggcagcgg cttcgtcgtg cacgccgcca cccggcgcgt gctcctgcac caccaccgcc
     1381 ggctcgacgc gtggctccag atgggcggcc acgacgaggg cgagagcgat ccgcgggcga
     1441 cggcgctccg cgagagcgcc gaggagtcgg gcctgacgga cctcgtcctc ctctcgcccg
     1501 agattctcga cgtcgacatc cacccgatcc ccgcgggaaa gggcgagccc ccccacctcc
     1561 accacgacgt gcgctacgcg ctcgcgacgc gcgcgcccga cgcgatccgc cgggacgacg
     1621 ccgagtcgat cgctctgcgc tggttcacgc tcgacgaggc tgcggccgcg atggccgacc
     1681 ccgacgcggc ccgcgcgctc gaccggatcg cgcggctgct ggacggcgtt tcgtccggcc
     1741 ggtaagagcc gtcagcgccc ttcgagcgcg tcggcgttcc tgtcttcgaa ggccgccgcg
     1801 tcggcgtaca tctcctcgat gacgtcgcga tacttcgagg cgacgacgcg gcgcttgatc
     1861 ttcatcgtcg gcgtcagctc gccgccctcg atcgagaagt cctccgtgag gaggcggaag
     1921 gcgcggatct gtcgctcgtg cggctcgtcg ccgttcagga tctcgatcgc ctgcttgaac
     1981 agcgcgagca ccttcggctc ctccacgagc tcctggcggg tccggtagga gacgttcgcc
     2041 cccgccgccc agccctcgag cttctcgaag ttcggcacga tgagagccga cagaaacttc
     2101 tgcctgtcgc cgatcaggac ggccgaggag acgaagcgaa ccgccttgag cgcggcctcg
     2161 atcggggccg gcgcgatgtt cttgccgtag gcgttgatca ggatttcctt cttgcggtcg
     2221 gtgagcgtga ggaagccgtc gaggtcgaac gagccgatgt cgccggtgag gaaccagccg
     2281 tcggcgtcga agacgctggc cgtctcctcc ttcttccgcc agtagccgcc gtccatgacg
     2341 ttcggccctt tcgcgaggac ctcgccgtcc ggagcgatct tgagcgtgac gcccggaatc
     2401 gcgcggccga cggtgccgag acgccactcg cccggccggt tgcacgagag gacggggctc
     2461 gtctccgtga ggccgtagcc ctcgtagacc tcgacgcccg ccgcccagaa gaacgccgcg
     2521 acgtcgtgcg cgaggggtgc gccccccgag atcgcgaaca ggaaccggcc gccgagccgg
     2581 gccttgatct tcccgaagac gaggcggtcc gcgatccggt acttgagccg gaggaagaac
     2641 ggcggcttct cgccggactc cacggcccgg aggtgcatcg cgccgatccg cacgccccat
     2701 tcgaagatcg cgcgccgcag ggccggggcg gcgtccacgg cgttccggac gcgcgcgagc
     2761 atcttctcgt agacgcgcgg gacggcggcg aagcagtgcg gccggatctc ggcgaagttc
     2821 tcggagagct tgtcgatcga ttccgcgtac gcgatcgtgg tcgcccgcga gaagaacagg
     2881 tagtcgacca tccgctcgaa gacgtgcgag agcggcagga aggagagcgc gaccatgtcg
     2941 ggcgtgacgt cgaagagcgt cgagcaggtc gcgacgttcg cgacgaagtt cccgtgggtc
     3001 aggatggcgc ccttcggttc gcccgtcgtg cccgacgtgt agatcatcgt cgcgaggtcg
     3061 gtcgggagga gggacgaggc ccgctcctcc cacgcctcgg ggtccgcggc gagggccgag
     3121 agccccttct ggaccacggt ctcgaacggg acggcacgcc ccgcgctcca cggaacctgc
     3181 tcgaggacga cgacgtgccg gagttccggg cagcggtcgc gcacgtcgag caccttctgg
     3241 agttcgatcg cgttcgagac gacgacgacc ttcgcgagcg agtcccggag gatgtactcg
     3301 acctggggcg cgaggtacga ggtgtagatc gggacggtca gcgctcccag cgagagcgtc
     3361 gcgaagtccg tcatgggcca ttcgggcctg ttctccgaga gaatcgcgac gcgatcctca
     3421 cggtcgacgc cgaggagggc gagcccgagc gccagaccct tcgtccgctc ctcgaactcg
     3481 cctgcggaga cgggcttcca ggctccgtcc accttcgaga ccaggagctc cgccttcccg
     3541 ctcgccgcgg cgtgacggaa gtaatcgacg agtgtctgcg caggaagggc ttcgatcacg
     3601 tgcgtgtgcc gggcattatg ccgaatcgcc gcgctcctcc gctcacagga cggtgagccc
     3661 cacggaaagg acgtccgcgc agcccgaccg gatctcgtcg aggacggccg gccccgggcc
     3721 ctggtcgacc tggatccggg cgcaggcggc ctcggcgccg gcgaagacga cgttctcggt
     3781 ctgctggacg ttgatgccgg ccgccttcag cacgccgaag acgtgcgcga ggacgccgac
     3841 gcggtcccgg tggcggaccg tcaggaggtg ggtcgccggc gtgcggctcg cgaggttgac
     3901 cgcgttcgga atcgcgccgc tcgccgcgaa cgccgcgacg atgcgcacgg tctcggccgc
     3961 gatcgcgttc tgagcctggt ccgtcgacgc gccgacgtgg tgcgagccga cgacgccggg
     4021 gagccgcacg atgtcgtcct cgaacgggcc gctccccccg gcgggctcct tttcgaagac
     4081 gtcgagaccg gcccggaccc cgcgctccga gatcgcccgg cgcagggcgg ccgcgtccat
     4141 gatctcgcct cggctcgtgt tcacgaagaa ggctcccggc ttcagcgcct cgaagaactt
     4201 cgcgtccagg aagcccttcg tctccttcgt gagggcgcaa tggaccgaca cggcgtccga
     4261 agccgccgcg agcgcgtgga tcgagtcggc gcgcgacacg ccgaggcgct cggcgtggtc
     4321 gtcgtcgagc gatcggctcc aggcgacgac gggcatcccg aacgcctggg cgcgcgtcac
     4381 gacctcgagg ccgatcgccc cgaggccgat cacgccgagc gtccggcccg cgaggccctt
     4441 cgccttgccg tactcggcct tgttccacac gccgttccgg aggtcgacgg cctggtccac
     4501 gaggcgccgg tcgagagcga gaagaagccc ccaggccagc tccgcgaccg cgatcgcgtt
     4561 cttgcccggg cagttcgaga cggcgacggc gcgcgcggac gcggccgcga ggtcgatcgt
     4621 gttcacgccc gctcccgccc gcacgatcag ggagagcccg gaggccctgt cgatctcggc
     4681 cgcggtcacc ttcgtcgaac ggacgacgag gacgtccgcc ctcgactccg cgaggcgggc
     4741 gccgagcgtc tcgccctcga gcttcggctc gtagaggacc tcgcagccga tcgccttcaa
     4801 cccgtcgaga ccggactctt cgaacttgtc ggcgaccagg accttcatgt tcccctcggc
     4861 ccttgggctc agaagcggtg gacgaaaagg cccgagcgca gcttcggctc gaaccacgtg
     4921 gatttcggcg gcatcgtgac gcccgcgtcc gcgatcgcca tgacgtccga gatctgcgtc
     4981 gggtacatcg cgaacgcgac ggcgccgcgg cccgagcgca cgcgctcctc gagctccgac
     5041 gtgccgcgga tcccgccgac gaagtcgatg cgcgggtccg tgcgcggatc gccgatgccg
     5101 aggacgggct tgaggagacg atcctgcaga agagagacgt ccagccgaga gaccggatcg
     5161 tttccggtcg ccggctcgcg cggggtcagg gtctgccaat tttcttcgag gtaaagagag
     5221 aaggtcccgc ggcggcgcgg ctcgggcgat tcggcgggct gcacgtcgaa ccgttccctt
     5281 acccttcgaa gaaaatcttc tttccccatc cccccaagat ccttcacgac acggttgtac
     5341 gggaggatcg cgagctggcg gtccgggaag atcgccgccg ggaaccagtt caccggatgg
     5401 tcgtcggcga gcgggccctt cgcggccagc gtcgcgcgcg cgcgcgaggc gctcgcgcag
     5461 cggtggtggc cgtcggccac gtacaggagc ggcaccgccg cgaacgccga gacgaagaac
     5521 gcgacgtccg cctccgggac gagccacagc gtgtgccgga ccgcgtcggg cgcctcgaaa
     5581 tcgaactcgg gcgcctgggc cgtcacggcc gccacgcgcg cgtcgaccgc gtcgacggcc
     5641 cggcacgtca ggaagacggg ctcggcgtgg gcggactgcg tgagcaggtg gcggacacgg
     5701 tcgtcctcct tgtccttgcg cgtcttctcg tgcttgcgga tcacgtccgc gtcgtagtcg
     5761 tccacggagc acgcagccac gaagccgtcc tgagaccggc cccgccacgt ctggcggtag
     5821 atcaggagcc gcgggacggg gtcctcgacg agcgtcccgt cggcgtagaa gccgtcgagg
     5881 ttctccctcc cctttgcgta gacctcgtcg gcgtgaatgt cggtcgcctc ggggaggtcg
     5941 atctcgggac ggcagacgtg gagaaacgac acgggattgc cctccgcgag ggcgcgggcc
     6001 tccttcgtgt cgacgacgtc gtagggcacg gaggcgacgc gcgcggcgag cgccttcggc
     6061 gggcggccgg aacggaacgg tcggatgcga gccatgggag gagagtatca acgggaggcc
     6121 cccgccggtg gcctccggag ggccggactt gcgccgggtg ctagactttt tcgatcgatc
     6181 ggaccccggt caggtgaaag gaacgcgcgg aatgaaactg tacgtcggca acctctccta
     6241 cggcgtgaac gatgcttccc tccgggccct cttcgaggcg tacggcgcgg tggagtccgc
     6301 caccgtcgtc gccgaccgcg actcgggccg ttccaagggc ttcggcttcg tggagatgag
     6361 cgacgcggac gcccagaagg cgatccaggc gctcaacggc tcccagcacg acggccgcac
     6421 gatccgcgtg aacgaggcgc gcccgaagac cgacgcgccc cgcgggccga ggggcccccg
     6481 ctgatcctta cgggcgcgcc cggcggcgcc ccgcattcgg tcaagagaat cctgacggcc
     6541 gcgatcctcc cgatcgcggc cttttttctc ctggccggag ccgggccctc cggcgacgac
     6601 ctcgtcttca ccggggcgcg ggtcctcgac ggttcgggcg ctccctggtt ccgtgccgac
     6661 gtcgccgtcg cgggcgggcg ggtcgcagcc gtcggctcgc tgcccgaggc ccgcctctcc
     6721 cgcgccgcgc ggcggatcga cgcgtccggg ctttacctcg cgcccggctt catcgacctc
     6781 ctcgggcagt ccgagtacaa cgtcctcgtc gacccgaggg ccgcctcgaa gatcacgcag
     6841 ggcatcacga cggaggtcac gggcgaaggc gacgcgatcg ccccgaacga cgagaagatg
     6901 ttccgggagg ccgaaccgac gtggaagcgc tacggcgtcc gcgcggactt cttcacgctc
     6961 gcggactact tcgcgcgcct cgcgaaggcc ccgcccacga tcaacctcgg gacgttcgtg
     7021 gggagcggcg ggctgcgcca gctcgtcgtc ggcggcgaga accgccgcgc gacgccggcc
     7081 gaagtcgcgc gcatggccgc ggaggtcgaa aaggcgatgc aggacggcgc cctcggcctc
     7141 tcgtcgtccc tccagtacgt gcccgacatc tacaactccg ccgacgaaat cgtcgcgatg
     7201 gcgagggcgg ccgcgaagca cggcggcgtg tacttcgtgc accagcgctc cgagagcaac
     7261 gcgatcgacg cgtcgctcga cgaggtcttc cggatcgcgc gcgaggcgaa gatcccggcg
     7321 aacgtctggc acctgaagac ggcctacaag cgcaactggg gtcggatgcc cgccgttctc
     7381 gcgcgcctcg aagcggcgcg cgccgaaggg ctcgacgtcg ccgcgaacca gtacccgtgg
     7441 accgcggcct cgaacccgct ctcctcgtgc cttccgccgt ggatgcgcga gggcgggaac
     7501 gagaagctcc tcgcgcgcct gaaggacccg gccctgcgcg agaaggcgaa ggcggacatg
     7561 ggccgggacg cggacgactg gcagaaccag tacttcggca gcggcgggcc gtcgcggatc
     7621 atcgtctcgg cggtgctcac gccggcgctc aaggcgctcg aaggcaggac gatcgaggag
     7681 atcgcggccg cccggaagaa ggacgcgcgc gacgccctga tggacgtcgt cctcgaggac
     7741 ggcggccact cgtcctgcat cttcttctgc atggacgagg aggacgtgaa gacggcgctg
     7801 aagcaccgcc tcgtgtcctt ctgcaccgac tcgggcgcgt ccgcgaccga cggcatcttc
     7861 tcggaggaga agtcccaccc gcgcggctgg gcctccgccg cgcgcatcct cggaacgtac
     7921 gtgcgggacg agaaggtcct gccgctcgag gacgccgtcc ggaagatgac gtcgttcgcg
     7981 gcggcgcgcg cgggcctgaa ggaccgcggc cttctccggg aaggctttcc cgccgatctc
     8041 gtcgcgttcg acctcgcgaa ggtgagggcg gtctcgacct acgcggaccc gctccgttac
     8101 tccgagggtt tcccctacgt ggccgtgaac ggcgttctcg tcgtggacgg gagccggatc
     8161 acctcggagc gcccaggacg ggcgctccga gggcccgggt tcgcgcgctg acggggcgct
     8221 accgggcggg aacgggcgcc gccgctccgg tcgccgggag gggcacccgc tgccacttct
     8281 tcatgaggtc gacgacgatg tcgccgattc ggaggaggtt ttcctggctc gatccgccga
     8341 ggtgcggcgt catgaggacg ttcggcgccg cgaggagcgg gctgttcgga tcgggcgggt
     8401 cgctcaggta gacgtccgtc gcgtaggcgc ggaccttgcc ggacgcgagg gcggccgcga
     8461 ggtcggcctc gacgacgatc gagccgcggg ccgtgttcac gaggatgacg ccgtctttca
     8521 tcttcgcgat cgtgtgctcg ttcacgagtc cgcgcgtctc ggccgtggcc gggacgtgga
     8581 agctgatgaa gtcggcccgc ctgaagagct cgtcgaggtc gtccacgagc tcggcgagcg
     8641 cgtggttcgc gatctgcggg tcgtacgcga ggaccttcat cccgaaggcc tgcgcgcggc
     8701 gggcgacctc ggtgccgatc aggccggcgc ccacgaggcc gagcgtcttg ccgaagagct
     8761 cggtgcgctt gagctccttc ttgaggaagc ggccgccctt gagcccgacg tgcccctcga
     8821 cgacccggct cgggaccgcg agcatgagcg ccagcgtgag ctccgccacg gcgaccgaag
     8881 acgcgcgcgg cgtgttcttc acgtcgatgc cgcgctcgcg gcaggcctgg atgtcgacgt
     8941 tgtcgaggcc gacgccgccc cggatcacga gcttgaggtt cggcgcgttc gccaggtagt
     9001 cgcgcgtgac cttcgtcttc gagcgcacga ggacgacgtc ggcctccttg aggcgcgcgg
     9061 cgtcggtgag gacctcgccg aacggggcga ggcgtttggg aagcgattcg tcgaacgaat
     9121 cggcgagcag gatcttcatg tgggcctcca accttcgggc acgccccgcc tcgggcgggc
     9181 ggacgcgtcc ctctacatgc tggggaccgg acgcatgtgc ggccatgatg aacccgtatc
     9241 aggggccgcg acggccccgg gtcagcgcgt cgcgggcggg cttccccggc cctccgccca
     9301 cgcgagaagg cgagcggcgt ccgggtcgcg cggcgagagc tggaccgcct cccggagcca
     9361 cggcagcgag cgcgccgtct ccccgcggcc gagatagccg gccgcggaac ggacgaggag
     9421 gcgcgggacc atcggcggga ccgccgggag ccccaggacg cggcggagcc accagggaga
     9481 aaccgacgcg gccgcatccg ggggcggggt tccttccgtg acgccgc
//
LOCUS       DAOMFY010000138         9515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2192_length_9515_cov_6.642600, whole genome shotgun sequence.
ACCESSION   DAOMFY010000138 DAOMFY010000000
VERSION     DAOMFY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2192_length_9515_cov_6.642600"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLB01_11735"
     CDS             <1..721
                     /locus_tag="PLB01_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78016.1"
                     /translation="SVRGLTKRFGGLTALDDVSFDVAPGEIFGVIGPNGAGKTTLFSC
                     LVGALAPTAGEIRFRGRRIGGLANHAVVRRGLVRTHQIVRPFRDMSVEDNVSIGAHFG
                     SGKRRGADARRRTAEALERTGLSSRARDLAGTLPIGDLKRLEIARALAAEPACLCLDE
                     VMGGLNPSEIVAAMSLVRSIRDSGVTILMIEHHVHAVAGVTDRILVLNFGEKIAEGPP
                     HDVLVDPAVVSAYLGDEEAAS"
     gene            718..1437
                     /locus_tag="PLB01_11740"
     CDS             718..1437
                     /locus_tag="PLB01_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78017.1"
                     /translation="MSLLAVEEIDVFYGDVQAVRGVSFSVEPGEVVTLIGANGAGKST
                     TLKTLAGLLAPRRGRIVFDGGDVAGSPAHALAARGLVLVPEGRQLWPGMSVLENLRMG
                     AYPRAARAKGDATLEEVFAMFPVLKRRIHQKAGTLSGGEQQMCAIGRGLMAQPRLLLL
                     DEPALGLSPLLVREVFATLREIRARGVTVLLVEQNVKHALALADRAYVMETGTITLSG
                     SADDLRNDDGVRDRYLGYSSV"
     gene            1484..3058
                     /locus_tag="PLB01_11745"
     CDS             1484..3058
                     /locus_tag="PLB01_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQQ78018.1"
                     /translation="MTTASAHDRLLGCLWRRAAHPAVVWGGRSYSFEDISRLSSLYSE
                     KLGALGIRRGDRVAVFAETCPELIAAFVGHLVSGVIHVPINTRYKADEARHILEDSGA
                     VAVLVGDEGEECGEVLREILRRCPEQLKHVIALREAFPAGQPPAAAPPSPSSPSKKIF
                     SSPSPSPPSDEDVAMLIYTSGTTGKSKGVALSYKALADNTASVTGLWRFAPEDRLVLA
                     LPLFHVHGLVLGVTGMLVNGLTLLLEPRFDPARVVEAFASEGATVLMGVPTMYVRLLE
                     HLDAHPEAADALRKARLFTAGSAPLPAADFEAFRAKTGHAILERYGMSETLFTLSNPY
                     DGERRPGTVGRATPGSSVRIVDDEGRDTADGELGEIVVKSDGMMSAYWGREADTAASF
                     RDGWFLTGDVARRAADGYVTIVGRKSVDVIKSGGYKIAAREIEDVLRRHPKVKDAAVV
                     GLEDRVWGQRIAAALVLADGCEPVAACAEVAAFAAEHLADYKKPRDVVALPELPRNAL
                     GKVQKHLLARVFPPKV"
     gene            complement(3055..5466)
                     /locus_tag="PLB01_11750"
     CDS             complement(3055..5466)
                     /locus_tag="PLB01_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ78019.1"
                     /translation="MKRFVLMAAAAALVSAPPAAAATSYRLPPKEVVQILDAPGPPQV
                     RLSPSGDAALLVEIEQNPPLSVVAEPFLRLAGVRLVPARGAQQHLLSASGLTVLSLPE
                     GRKTRVALPAAARLGMPVFSPDGNVFAFTRDTDDRTELWLGETKSGAAAAVAGIRVND
                     VAGEALRFSNDGKTLWILAIPQDRKPMPPEPAVPQGPVVEETAGKTAQPATFQDLLRS
                     SRDEDLFEHFATSQVLKLDVASKRTTAVGAPGLFTRLDPSPDGRYLLVARMKRPFSYR
                     VPYREFPHAVEIWDGAGRVVRLVADLPLADQTPRQGVPTGPRHVEWQPMEEASLFWTE
                     ALDGGDPLRKVPFRESLHTLRAPFAGEGREVRQLVHRLQGVDWTVTPGTAIVTEFDRS
                     RRWRTTSVIDLDNGSTPPRVLFDLSAQDAYKDPGRFVSGTSRRGRRTIVQEGVVAFLS
                     GEGATPDGARPFLDAVDLSTGQTRRLLKSPEDGLERFVGFLGENRTKIVIRRESASEP
                     PNLWLVDLAQHTRAKLTDFKDPAPQFSGVKKEILKYTRADGVALSGTLYLPAGYETGK
                     RLPCLVWAYPLEYSDAGTAGQVRSSPNAFTRPTGASPLFFLTQGWAVLMDATMPVVGD
                     PEKMNDTYVDQITGAARAAVDALDARGVVDRKRVVVGGHSYGAFMTANLLAHSDLFAA
                     GIARSGAYNRTLTPFGFQTERRSLWEAPETYLRVSPFMVADKIKAPLLLIHGEADNNS
                     GTFPIQSERLYAAIQGTGGTARLVLLPFEAHGYRARESVLHTLAEMLEWGARWTADKP
                     AASPN"
     gene            5517..6497
                     /locus_tag="PLB01_11755"
     CDS             5517..6497
                     /locus_tag="PLB01_11755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQQ78020.1"
                     /translation="MTLSAGQIAALFCALTAAAFGLVALTLLVASRTRRTLALYGALA
                     FATAAVLVLPILRPGRPHAVDNASAALVAALAAALLFDLGQGSRRTRAFLFAAASVLA
                     LAGLAETAQRHAFLLARTVVPPLGAGFIVFTVLLLPTVADEERRLFARATTDPLTGLL
                     NRAAFRERARAEIARAERTGRPLAVAMLDLDHFKAFNDAHGHPAGDAALSAVAGAITR
                     TIRAIDLAGRYGGEEFILLLVEADAVAAVRALERLRKSVAALEPPRVARPITISAGIA
                     VHVPPFEHSTLEGLVARADATLYAAKEAGRNKVMVEAPSRPAAASDVVYR"
     gene            complement(6505..7695)
                     /locus_tag="PLB01_11760"
     CDS             complement(6505..7695)
                     /locus_tag="PLB01_11760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQQ78021.1"
                     /translation="MRFAARASTVAGSATLEVMRTAAALRARGVDVIDLGPGEPDFPT
                     PDFIKAAGIAAIQSGFTRYTDAVGTLELRSAIAERYRREWKAPWGPGDVVVTNGGKQA
                     LHTAILALFEEGDDVLVPVPYWVSFPEMVKLAGARPVFVPTSPENGFRLAAADVEAAA
                     TPATRGILVNSPTNPSGAVIEPSEIEGIVRLCAARGWMFLFDECYDRFVFEGRHVSAA
                     AFAAEFPETVVLAGTFSKTYAMTGWRMGYACAARPVAELIGRLVGHATSNVCSVTQAA
                     ALEALRRPEESAAAIETMLAEYRRRRAYLIPALNEMPGFRCTPPGGTFYAFPDVSAWY
                     GKTVGGVAVTGSASFAKALLDGAAVAVTPGSAFGEDRCIRISFATSLERLDEALKRLR
                     GVLA"
     gene            complement(7692..8876)
                     /locus_tag="PLB01_11765"
     CDS             complement(7692..8876)
                     /locus_tag="PLB01_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78022.1"
                     /translation="MKSELPAAGAAIVAKASPLTALWTFPSVLFSAFLVAWGAEAAQF
                     LVSQGLALAILAWLQTLPEFAVEAVIAWEAGKDPAKVHLAIANFTGSIRLLVGLGWPL
                     VYFVAAFFARRTGKRKTWIAIEFDDEHSLEVMGLLPPLAWFAVIWWKGSLGLWDALIL
                     SLCYFAYLFALLRVPPREGDVEEIEDLPAVSKWALRQGPGRRGWAIAGIFVVGGLMLV
                     VSAHAFVESMLALAIAAGVAPFVFVQWVAPFLSEFPEKLSAFNWARQITKAPMAVMNL
                     VSSTINQWSILSALIPVIYSFSRGTPSAIVFDEHQRVEILLTILQSFLGWLLLVNLDL
                     RAFEAAILFVFWLVQFAVPSTRGAMVGVYAAWCGIEILMIAVGRRRPRAIGAAWRGLR
                     GS"
     gene            8918..>9515
                     /locus_tag="PLB01_11770"
     CDS             8918..>9515
                     /locus_tag="PLB01_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQQ78023.1"
                     /translation="MPRIESPTNPRIAAAIRAVAGGDRMLLEGKRMVEEALDAGVALD
                     EVFLVDATSEENEEFLRRLRGEGADAAGGAAPAARVIEVSARVLRRISDLPSTRGVAA
                     LAPPPHRSLASLYSPSKKRSSSDASRKAAATASRGSEAENSLFLLLDGIQDPANVGAI
                     VRCAEAFGVAGVVLTPGSAWPFSPRALRASAGSALRVPL"
BASE COUNT         1403 a         3447 c         3324 g         1341 t
ORIGIN      
        1 ctccgtccgc ggcctcacga agcggttcgg cggcctgacc gccctcgacg acgtctcgtt
       61 cgacgtcgcg cccggcgaga tcttcggcgt gatcggcccg aacggcgcgg gcaagacgac
      121 gctcttctcg tgcctcgtcg gcgcgctcgc gcctacggcc ggagagatcc gcttccgcgg
      181 ccggcgcatc ggcggcctcg cgaaccacgc cgtcgtccgg cgcgggctcg tccggaccca
      241 ccagatcgtc cggccgttcc gcgacatgag cgtggaggac aacgtctcca tcggcgcgca
      301 cttcggatcg ggaaagcgcc ggggcgccga tgcgcgcagg cggacggccg aggcgctcga
      361 gcgtacgggc ctgtcttccc gggcgcgcga cctcgccggc accctcccca tcggcgacct
      421 caagcgcctc gagatcgcgc gcgctctcgc cgccgagccc gcgtgcctct gcctcgacga
      481 ggtcatgggc ggcctcaatc cctccgagat cgtggccgcg atgtcgctcg tgcggagcat
      541 ccgggattcc ggcgtgacga tcctcatgat cgagcaccac gtccacgcgg tcgccggcgt
      601 cacggatcgc atcctcgtcc tgaacttcgg cgagaagatc gccgagggcc cgccgcacga
      661 cgtcctcgtg gaccccgccg tcgtttccgc gtacctcggc gacgaggagg ccgcctcgtg
      721 agcctcctcg cggtcgagga gatcgacgtc ttctacggag acgtgcaggc cgtccgcggc
      781 gtgtccttca gcgtcgagcc gggcgaagtg gtgacgctca tcggcgcgaa cggcgccggc
      841 aagtccacga cgctcaagac gctcgcgggc ctgctcgcac cgcgccgggg ccgcatcgtc
      901 ttcgacgggg gggatgtcgc cggctccccg gcccacgccc tcgcggcccg gggcctcgtc
      961 ctcgtccccg agggccgcca gctctggccg gggatgtccg ttctcgagaa cctccggatg
     1021 ggcgcgtacc cgcgggcggc gcgcgcgaag ggcgacgcga cgctcgagga agtcttcgcg
     1081 atgtttccgg tgctcaagcg ccgcatccac cagaaagcag ggacgctgtc cggcggcgag
     1141 cagcagatgt gcgcgatcgg gcgcggcctc atggcgcagc cccgcctcct cctcctcgac
     1201 gagcccgccc tcgggctgtc gccgctcctc gtccgcgagg tcttcgccac gctgcgcgag
     1261 atccgcgcgc gcggcgtgac cgtcctcctc gtggagcaga acgtgaagca cgcgctcgcg
     1321 ctcgcggacc gggcgtacgt gatggagacg ggcacgatca cgctttccgg atccgccgac
     1381 gacctccgca acgacgacgg cgtgcgggac aggtatctcg ggtattcctc ggtttagaga
     1441 agacaaagag aagaagaaga ctttcttaga aggtgaatag ggcatgacga ccgcctcggc
     1501 gcacgaccgc ctgctcggtt gcctttggcg ccgcgcggcg catccggccg tcgtgtgggg
     1561 cgggcgctct tactcattcg aagatatctc ccgtctttct tctctctact ccgagaagct
     1621 cggcgcgctc ggcatccgcc gcggcgaccg cgtggccgtg ttcgccgaga cgtgccccga
     1681 gctgatcgcg gcgttcgtcg gccacctcgt gtccggcgtc atccacgtgc cgataaacac
     1741 ccggtacaag gccgacgagg cgcggcacat cctcgaggac tccggggcag tggcggtgct
     1801 ggtgggggac gagggagagg aatgcggaga agtcctaaga gagattctta gaaggtgccc
     1861 ggagcaactg aagcacgtga tcgcgcttcg tgaggcgttc ccggctggcc agccgcccgc
     1921 cgcagctccg ccctccccct cttcaccttc taagaagatc ttctcttctc cttctccctc
     1981 tcccccctct gacgaagacg tcgccatgct catctacacg tcgggcacga cgggcaagag
     2041 caagggcgtc gcgctgtcgt acaaggcgct cgcggacaac acggcttccg tgaccggcct
     2101 ctggcgcttc gcgcccgagg accggctcgt cctcgcgctg ccgctcttcc acgtccacgg
     2161 cctcgtcctc ggtgtgaccg gcatgctcgt gaacggcctg acgctcctcc tcgagccccg
     2221 cttcgatccg gcgcgcgtcg tcgaggcttt cgccagcgag ggcgccaccg tcctcatggg
     2281 cgtgcccacg atgtacgtgc gcctcctcga gcacctcgac gcgcatccgg aggccgccga
     2341 cgcgctgcgg aaggcgcgcc tcttcacggc gggcagcgct cccctgcccg cggccgactt
     2401 cgaggcgttc cgcgcgaaga cggggcacgc gatcctcgag cgctacggga tgagcgagac
     2461 gctcttcacg ctctcgaacc cgtacgacgg cgaacgccgc cccggcaccg tcggccgcgc
     2521 gacgcccggc agttcggttc gcatcgtcga cgacgagggg cgcgacacgg ccgacggcga
     2581 gctgggcgag atcgtcgtga agagcgacgg gatgatgagc gcgtactggg gccgggaagc
     2641 cgacacggcc gcgtcgttcc gggacggctg gttcctcacg ggcgacgtcg cgcgccgcgc
     2701 ggcggacggg tacgtcacga tcgtgggccg caagtccgtg gacgtcatca agagcggcgg
     2761 ctacaagatc gccgcgcgcg agatcgagga cgtcctccgc cgccacccga aggtgaagga
     2821 cgccgccgtc gtcggcctcg aggaccgcgt ctggggccag cggatcgccg ccgcgctcgt
     2881 cctcgccgac ggctgcgagc ccgtcgcggc ctgcgccgag gtggcggcgt tcgcggcgga
     2941 gcacctcgcg gactacaaga agcctcgcga cgtcgtcgcc ctgccggagc tgccgcgcaa
     3001 cgccctcggg aaggtgcaga agcacctcct cgcgagggtc ttcccgccga aggtctagtt
     3061 cggcgaagcc gccggcttgt ccgccgtcca gcgcgcgccc cactccagca tctcggccag
     3121 cgtgtggagc acgctctcgc gcgcccggta gccgtgcgcc tcgaacggga ggagcacgag
     3181 gcgcgcggtg ccgccggtgc cctggatcgc ggcgtagagg cgctccgact ggatcgggaa
     3241 cgtgcccgag ttgttgtcgg cctcgccgtg gatcaggagg agcggcgcct tgatcttgtc
     3301 cgccaccatg aacggcgaga ccctgaggta agtctcgggc gcctcccaga ggctgcgccg
     3361 ctcggtctgg aagccgaacg gcgtgagcgt gcggttgtag gcgccgctgc gcgcgatgcc
     3421 ggccgcgaag aggtcgctgt gcgcgagcag gttcgccgtc atgaaggcgc cgtagctgtg
     3481 gcccccgacc acgaccctct tccggtccac gacgccgcgg gcgtccagag cgtcgaccgc
     3541 cgccctcgcg gcgcccgtga tctggtcgac gtacgtgtcg ttcatcttct cggggtcgcc
     3601 gacgacgggc atcgtggcgt ccatcaggac ggcccatccc tgtgtcagga agaagagcgg
     3661 cgaggcgccc gtgggccgcg tgaacgcgtt cggcgaggag cgcacctgcc ccgccgttcc
     3721 ggcgtccgag tactccagcg gatacgccca gacgaggcac gggaggcgct tgccggtctc
     3781 gtagccggcg ggcaggtaga gcgtgcccga gagcgcgacg ccgtcggcgc gcgtgtactt
     3841 caggatctcc ttcttcacgc cggaaaactg cggcgcgggg tccttgaagt ccgtgagctt
     3901 cgcgcgggtg tgctgcgcga gatcgacgag ccagaggttc gggggctcgc tcgcggactc
     3961 gcgccggatc acgatcttcg tccggttctc cccgaggaac ccgacgaaac gctccagccc
     4021 gtcctcgggt gacttgagaa ggcgccgcgt ctgcccggtg gagaggtcca ccgcgtcgag
     4081 gaacggccgg gcgccgtccg gcgtcgcgcc ctcgcccgag agaaacgcca cgacgccttc
     4141 ctgcacgatc gtccggcggc cccggcgcga cgtcccggaa acgaagcggc ccggatcctt
     4201 gtacgcgtcc tgcgcggaaa ggtcgaagag gacacgcggc ggcgtcgagc cgttgtcgag
     4261 gtcgatcacg gacgtcgtcc gccagcggcg gctgcggtcg aactcggtca cgatcgccgt
     4321 tcccggcgtc acggtccagt ccacgccctg caggcggtgg acgagctggc ggacctcgcg
     4381 cccctcgccc gcgaacggcg cgcgcagcgt gtgcagcgat tcccggaacg ggaccttacg
     4441 gagggggtcg ccgccgtcga gggcctcggt ccagaaaagc gaggcctcct ccatgggctg
     4501 ccactcgacg tgccgcggac ccgtcgggac gccctgccgc ggcgtctggt cggcgagcgg
     4561 gaggtcggcc acgaggcgaa cgacacggcc cgcgccgtcc cagatctcga cggcgtgcgg
     4621 gaactcgcga tacggaacgc ggtacgagaa ggggcgcttc atccgcgcca cgaggagata
     4681 ccgcccgtcc ggagacgggt cgagacgcgt gaagaggccc ggcgcgccga cggccgtcgt
     4741 gcgcttcgac gccacgtcca gcttgaggac ctgcgaggtc gcgaaatgct cgaagagatc
     4801 ctcgtcccgc gagctcctga ggaggtcctg gaacgtggcc ggctgggcgg tcttcccggc
     4861 cgtctcctcc acgaccggcc cctgcggcac cgcgggctcg ggcggcatcg gcttgcggtc
     4921 ctgcgggatc gcgaggatcc acagggtctt cccgtcgttc gaaaaccgca gcgcctcgcc
     4981 cgcgacgtcg ttcacgcgga tcccggccac cgcggcggcg gcgcccgact tcgtctcgcc
     5041 gagccagagc tccgtccggt cgtccgtgtc gcgcgtgaag gcgaagacgt tcccgtccgg
     5101 cgagaacacc ggcatcccga ggcgggcggc ggcggggagg gcgacccggg tcttccggcc
     5161 ctccggaagg gagagcacgg tgaggccgga cgccgacagg agatgctgct gggcgccgcg
     5221 cgccgggacg aggcgcactc ccgccagcct gaggaaaggc tcggcgacga cggagagcgg
     5281 cgggttctgc tcgatctcga cgaggagggc cgcgtcgccg gaggggctca gccgcacctg
     5341 cggcgggccg ggggcgtcga ggatctggac gacttccttc ggaggcagcc tgtaggacgt
     5401 cgccgcggcg gcgggcgggg ctgaaacgag ggcggcggcg gccgccatga gaacgaatcg
     5461 cttcatggga atctcccccg cgatgatggc acgccgaacg tagaatcgtc ctcgccatga
     5521 cgctctcggc cgggcagatt gcggcgctct tctgcgcgct cacggcggcc gctttcggcc
     5581 tcgtggccct cacgctcctc gtcgcctccc ggacccggcg gaccctcgcg ctctacggcg
     5641 cgctcgcgtt cgcgacggcc gccgtcctcg tcctcccgat cctcaggccc ggcaggcctc
     5701 acgcggtcga caacgcgtcc gcggctctcg tcgccgccct cgccgccgcg ctcctcttcg
     5761 acctcggcca gggctcccgc cggacccggg cgttcctctt cgcggccgcg agtgtcctcg
     5821 cgctggccgg cctcgccgag accgcgcagc ggcatgcgtt tctcctcgcg cgtaccgtcg
     5881 tcccgccgct cggcgcgggc ttcatcgtgt tcacggttct gctcctcccg acggtcgcgg
     5941 acgaggagcg ccgtctcttc gcccgggcca cgacggaccc tctcaccggc ctcctgaacc
     6001 gcgccgcgtt ccgcgagcga gcgcgcgcgg agatcgcgcg cgccgagcgg acgggccggc
     6061 ccctcgcggt cgcgatgctg gacctcgacc acttcaaggc cttcaacgac gcccacgggc
     6121 atccggcggg agacgccgcg ctctcggccg tggcgggggc gatcacgcgc acgatccgcg
     6181 cgatcgacct cgccggccgc tacggcggcg aagagttcat ccttctcctc gtggaggccg
     6241 acgccgtcgc ggccgtgcgc gcgctcgaac ggctccgcaa gtccgtcgcg gccctcgaac
     6301 ccccgcgggt cgcccgcccg atcacgatct ccgccggcat cgcggtccac gttcccccgt
     6361 tcgagcactc cacgctcgag ggactcgtcg cccgcgccga cgcgacgctc tacgcggcca
     6421 aggaagcggg ccgcaacaag gtgatggtcg aggcgccgtc ccggccggcg gccgcctccg
     6481 acgtcgtcta tcgctgaacg ggcgtcaggc gagaacgccg cggagccgct tcagggcttc
     6541 gtcgagccgt tcgagcgacg tcgcgaagga gatgcggatg caccggtcct ctccgaacgc
     6601 cgaaccgggc gtgacggcga ccgcggcgcc gtcgaggagg gccttcgcaa acgacgcgga
     6661 tcccgtcacg gcgacgccgc cgaccgtctt tccgtaccat gcggaaacgt ccgggaacgc
     6721 gtagaaggtg ccgcccggcg gcgtgcaccg gaagcccggc atctcgttca atgccgggat
     6781 gaggtacgcg cggcggcggc ggtactcggc gagcatcgtc tcgatcgccg ccgcgctttc
     6841 ctcggggcgc ctcagggcct cgagcgccgc ggcctgcgtc acggagcaca cgttcgacgt
     6901 cgcgtggccg acgagcctgc cgatcagctc cgccaccggc cgcgcggcgc acgcgtagcc
     6961 catgcgccag ccggtcatcg cgtacgtctt gctgaacgtc cccgcgagga cgaccgtctc
     7021 cggaaactcc gcggcgaacg ccgccgccga gacgtgccgg ccctcgaaga cgaaccggtc
     7081 gtagcactcg tcgaagagaa acatccagcc gcgcgccgcg cagagccgca cgatcccctc
     7141 gatctccgaa ggctcgatga cggcgcccga cgggttcgtc ggcgagttca cgaggatccc
     7201 gcgggtcgcg ggtgtcgccg cggcctccac gtcggcggcc gcgagccgga agccgttctc
     7261 cggcgacgtc ggcacgaaga cgggccgcgc cccggcgagc ttcaccatct ccgggaacga
     7321 cacccagtac gggacgggga cgaggacgtc gtccccctcc tcgaagagcg cgaggatcgc
     7381 ggtgtggagc gcctgcttgc ccccgttcgt cacgaccacg tcgcccggcc cccagggcgc
     7441 cttccactcg cgccggtagc gctcggcgat cgcggaacgg agctcgagcg tccccacggc
     7501 gtccgtgtag cgcgtgaaac ccgactggat cgcggcgatg ccggccgcct tgatgaagtc
     7561 cggcgtcggg aagtccggct cgccggggcc gaggtcgatg acgtcgacgc ccctcgcgcg
     7621 cagcgccgcg gcggtcctca tgacctcgag cgtcgcggag ccggcgacgg tcgacgcgcg
     7681 ggcggcgaat ctcatgatcc tctcaggccc ctccacgcgg cgccgatcgc gcgcggcctg
     7741 cggcggccga ccgcgatcat gaggatctcg attccgcacc acgcggcgta gacgccgacc
     7801 atcgcgccgc gcgtcgacgg caccgcgaac tggacgagcc agaacacgaa caggatggcc
     7861 gcctcgaacg cccggaggtc gaggttcacg agcaggagcc acccgaggaa gctctggagg
     7921 atcgtgagca ggatctccac gcgctggtgc tcgtcgaaga cgatcgcgga cggcgtgccg
     7981 cgcgagaacg agtagatgac cgggatgagg gccgagagga tcgaccactg gttgatggtg
     8041 gacgagacga ggttcatcac ggccatcggc gctttcgtga tctggcgggc ccagttgaac
     8101 gccgagagct tctccggaaa ctccgacagg aacggggcga cccactggac gaagacgaac
     8161 ggcgcgacgc ccgccgcgat cgcgagcgcg agcatggact cgacgaaggc gtgggcggag
     8221 acgacgagca tcaggccgcc gacgacgaag atcccggcga tcgcccagcc gcgccggccc
     8281 ggcccctgcc gcagagccca tttcgacacc gcaggcaggt cttcgatctc ttcgacgtcg
     8341 ccctcgcgcg gagggaccct caggagcgcg aagaggtacg cgaaatagca cagggacagg
     8401 atgagcgcgt cccacaggcc gagcgagccc ttccaccaga tgacggcgaa ccacgcgagc
     8461 ggaggcagga ggcccatgac ctcgagcgag tgctcgtcgt cgaactcgat cgcgatccac
     8521 gtcttccgct tgcccgtccg gcgcgcgaag aacgccgcca cgaagtagac gagcggccag
     8581 ccgaggccga cgaggaggcg gatcgagccc gtgaagttcg cgatcgcgag atgaaccttg
     8641 gcgggatcct tcccggcctc ccacgcgatg accgcctcca ccgcgaactc gggcagcgtc
     8701 tggagccacg cgaggatcgc gagggcgagc ccctgcgaga cgaggaactg cgccgcctcg
     8761 gcgccccagg cgacgaggaa cgccgagaag aggacggacg ggaacgtcca cagcgccgtc
     8821 agcggggacg ccttcgcgac gatggccgcc ccggccgccg gcaactccga cttcattcac
     8881 ccggatttaa gcacggcgcg ggctagactt ccgccgaatg ccccggatcg agtccccgac
     8941 gaacccgcgc atcgccgccg ccatccgcgc ggtcgctggg ggcgaccgca tgctcctcga
     9001 gggaaagagg atggtcgaag aggcgctcga cgcgggagta gcgctcgacg aggtgttcct
     9061 cgtcgatgca acgtccgaag agaacgaaga gtttcttaga aggttaagag gggaaggagc
     9121 cgatgcggcc ggcggcgcag cgcctgcggc gcgggtgatt gaggtgtcgg cccgcgtcct
     9181 tcgaagaatt tccgatctcc cctcgacccg gggcgtcgcg gctctcgcgc cgccccccca
     9241 tcgctcgctg gcctctctgt attcaccttc aaagaaaagg tcctcttcgg acgcttcccg
     9301 aaaggcagcc gcgaccgcct cgagagggtc cgaagcagag aattccctct tcctcctcct
     9361 cgacggcatc caggaccccg cgaacgtcgg cgccatcgtc cgctgcgcgg aggcgttcgg
     9421 cgtggccggc gtggtcctga cgccggggtc cgcgtggccg ttctcgccgc gggccctgcg
     9481 cgcgtcggcg ggcagcgcgc tgcgcgtgcc gctcg
//
LOCUS       DAOMFY010000139         9359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2238_length_9359_cov_7.497098, whole genome shotgun sequence.
ACCESSION   DAOMFY010000139 DAOMFY010000000
VERSION     DAOMFY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2238_length_9359_cov_7.497098"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1083
                     /locus_tag="PLB01_11775"
     CDS             247..1083
                     /locus_tag="PLB01_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78024.1"
                     /translation="MGPALTLSRLPGTIPLVFAAYAEGAADLSFESALARTALDRVQA
                     YFGDAPIRQYTVQLELLTPLEGHDYGFSQEHVDSGTFSLSAERATTAQTGADARERTL
                     VNYAHHMAHSWIPKRAYGAGYSPHTWEAPPVIDTIWFNEGFGRYAAVQALADGMPPAE
                     SAAFRTRTLARLRAILKEAPPFVRRMPLAELSRSASFLYSADFRIGMSTFARGALMAA
                     EMDDRIRAATGGEKSLRDGLRALLKAPQPFRTEELPVIFRGSTGVDVREILDRWMKET
                     TP"
     gene            1080..1616
                     /locus_tag="PLB01_11780"
     CDS             1080..1616
                     /locus_tag="PLB01_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQQ78025.1"
                     /translation="MKRTLALLVGAVLAAAGCSSLSISTDYDKAADFSSYKTFTWKDT
                     GELRDSLWSKRFEGVFSDTLASKGLRRVDSGGDLWVAAHPRLSKETQINTYNTGWGYG
                     YGWYGYGGMGMATTTVTEIPVGTLMIDLVDAKKKELVWRGVASDTLNTDPNRTAEDRE
                     KKLREVAAKMFEGYPPKK"
     gene            1645..2184
                     /locus_tag="PLB01_11785"
     CDS             1645..2184
                     /locus_tag="PLB01_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78026.1"
                     /translation="MKTITGFLLLALAGSASAAETFTATAEAKNAAGASKSAPITITY
                     SALSTPAERDALFAALKSGGHEAAKKVLAGMKDIGTIEGPKKKVAVKYAFARPVGSGR
                     MVTVIAAEPMNAIKSDLSTTPPKPGFDLTIALLVLDAGDKGSGEFTPAAKIKLREDGA
                     LTTEDSSTETIWLKGVAKK"
     gene            complement(2243..3847)
                     /locus_tag="PLB01_11790"
     CDS             complement(2243..3847)
                     /locus_tag="PLB01_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQQ78027.1"
                     /translation="MTLRRSMTLALLSSTLAASAAFAQAPKAKAAPAAKPAAAPKAAP
                     AGKPNILVIWGDDIGGFNISAYNRGMMGYRTPNIDRIAADGALFTDWYGQQSCTAGRA
                     AFVTGQSPIRTGLTKVGLPGAPEGMKKEDPTIATVLKAQGYATGQFGKNHLGDADATL
                     PTNHGFDEFFGNLYHLNAEEEPENPDYPKNPEFKKKFGPRGVIHSFADGRISDTGPLT
                     RKRMETIDEEVTAKALAFMENAKKSGKPFFLWWNSTRMHIFTHLKKESEGKTGLGVYA
                     DGMVEHDGHVGQVLAKLHDLGLDDTTIVMYSTDNGAETFTWPDGGTTMFRGEKNTNWE
                     GGYRVPAMIRWPGVIKPGTVINEIAAHEDMLTTFAAAAGDKTVKDDLLKGRTMGGATY
                     KVHLDGYDLGPALRGEAAWPRKEFIYWTDDGQVAALRWENWKITFLEQKGEGLRVWQE
                     PFTPLRAPLITNLRMDPFERAEAEHAMGYQRWFMEHMFAIAPAGGYVAQWLQSFKEFP
                     PRQKPGSFNLERVMESVTGGAQKSAN"
     gene            3993..4958
                     /locus_tag="PLB01_11795"
     CDS             3993..4958
                     /locus_tag="PLB01_11795"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQQ78028.1"
                     /translation="MHRLMPDLRLLGHAVALAEHAHFGKAAESVGLSQPAFSRSIQAL
                     ERHFGCRLFERDRGRAVPTPYGRIAVERARQLLHGASELQREIGLLRGADTGELRVSV
                     ASYPAELSVPAALQRLLAAHPALTVRVRVRDWQDLVTDVRGETVDLGIGEISELDGEA
                     HLETELVGSHRVSFICRPGHPLRRKRRVTLGDMVAYPFAGTVWPPRAARMLPPPPFAA
                     GAIDAETGRFVPAINVDRPLNVKDLVRETNSLAYLPRTLCEAELFSGTLAVVPVSVSW
                     ARLNYGFIWRRGRMRSPAAVAFSAAVRAAEAGVVAREADLAHRFP"
     gene            5014..5856
                     /locus_tag="PLB01_11800"
     CDS             5014..5856
                     /locus_tag="PLB01_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQQ78029.1"
                     /translation="MRRLLREPLVHFLAIGAALFLVFAWKGGAGASSGRIVVTRARLE
                     SLAAGFARTWQRPPTAEELKGLVDGYVREEIAVREAMATGLDRDDTIIRRRLKQKVDF
                     LAEDRIDAAPPGEAELSAWLTAHPDLYRAEERVSFRQVCLTPEKRGGPAAAAAEAKRL
                     AAALEKKGPGAEAAGDSLLLPPEMPLAPKGEIARVFGSEFADAVVKLEPGRWTAPVPS
                     GFGVHAVLVLERTAARAPALADVRSAVERDFTADRRTRELDALYARLLAKTKVVIETA
                     PEKK"
     gene            5853..6860
                     /locus_tag="PLB01_11805"
     CDS             5853..6860
                     /locus_tag="PLB01_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006854068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQQ78030.1"
                     /translation="MRARGGLGLVLLAALAAAPPALAHENRPGFLEMEETSPGVYAFL
                     WKAPFGGETEIHIAPVLPPGCVLATSGGSQLTTSAVLVRGTLRCEGGLAGKRIVIDGL
                     SSTATDVLVRVHRADGVLESHLVGPAAPSVTLGSATTGAQRAASYLKLGVEHILMGVD
                     HLLFVLGLLLIVSDRWMLVKTITSFTVAHSLTLAVATLGFASAPLPPLNAAIALSILF
                     LGPEIVRTWRGETSFTIRHPWVVAFAFGLLHGFGFASGLTAMGLPKAEIPLALLLFNV
                     GVEIGQMFFVAVILLLTRSFSILEIRWPRWAEALPGYAVGSLGAYWTIQRTVMLLGGA
                     R"
     gene            6857..7471
                     /locus_tag="PLB01_11810"
     CDS             6857..7471
                     /locus_tag="PLB01_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQQ78031.1"
                     /translation="MKATRAFRGSSPEALLALLVLVLAVSPARAHIQAGEAAGFLSGF
                     RHPVSGLDHVLAMVSVGLWGAQLGAPAVWLLPVVFPMVMAFGGFLGLAGVPLPGVEIA
                     IALSALLLGLAVAREARPPLALAAALVGFFAIFHGHAHGTELPPGQSGILYSIGFVVA
                     TGLLHLSGIAIGLIHRWSWGRVALRVAGSLVAIAGFGFLWKALR"
     gene            7468..8109
                     /locus_tag="PLB01_11815"
     CDS             7468..8109
                     /locus_tag="PLB01_11815"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQQ78032.1"
                     /translation="MRRRVPGAGILAFFAALLISGRAEAHLVTTGLGPVYDGIGHLVL
                     TPEDLVPIVALALYAGLRGARAGRLALFVLPVAWLVGGLLGLRFGGAASAAAPYPALS
                     FILLGLLVAADARLPDAAVAALATALGLVHGFLNGPALAGTGPGALGLVGIAAVAFVM
                     IALVSALVVSLKAPAARIAVRVAGSWIGAIGLLLLGWALRTRGVPPGPGMLAP"
     gene            8106..8885
                     /locus_tag="PLB01_11820"
     CDS             8106..8885
                     /locus_tag="PLB01_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HQQ78033.1"
                     /translation="MSNVAGALLTFAGFFLATLAFIEAGLRAGRRALAGPDSSRPAGL
                     GTVETVAFGLLGLLLAFTFSGAASRFEARRALIVEEANDIGTAWLRLDVLPPAFQPPL
                     RDAFRRYTDSRILTYAKVAAGDIAGGRAEYARGVAIQNEIWAGAVAACRAAPSQATIV
                     LFPALNAMIDITTTRMAATDMHPPKIIYFVLGGISLVCALLVGYEMGASTARSWLHVV
                     GFAAILAFTLYVILDFEYPRIGLIRVEDFDKYLVQVRASMK"
     gene            8882..>9359
                     /locus_tag="PLB01_11825"
     CDS             8882..>9359
                     /locus_tag="PLB01_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78034.1"
                     /translation="MKSFSARAWIAVLALLAAARFATMATDAWHWDEVLLADAVAHGI
                     DLRVHRPHPPGYPLLVETAALVHGTGVDPYRSLALVGTAGGLLAAAALAAFLAAAGLG
                     ADFACLGGLLYAVVPSVWLFGVRGFSDAPAAAAVFASAALLLSAAERRLPGRAAAGF"
BASE COUNT         1229 a         3443 c         3154 g         1533 t
ORIGIN      
        1 cggtcgcgcc cggcgcggcg gacgccggcc cgccgctcac agaggccgcg gagatatgcc
       61 ccctgtccgg caagctgcgt ggaccgcact gcccgcattc ccaggtcgag atcttcccgg
      121 ccacgggcgc cgccggaaag gcccccacgg ccgtctgcga catgcacgag cgcgtccgcg
      181 tggatcgccg gcacggcctc cgcgcgggcg cccgactacg acacgctcgc ggactcgcag
      241 gtcctcatgg gcccggccct gacgctctcc cgccttcccg gcacgatccc gctcgtcttc
      301 gcggcctacg ccgagggcgc ggcggacctc tccttcgaga gcgcgctcgc gcgcacggcc
      361 ctcgaccgcg tccaggcgta cttcggcgac gcgccgatcc ggcagtacac cgtccagctc
      421 gagctcctga cgccgctcga gggtcacgac tacggcttca gccaggagca cgtcgacagc
      481 gggacgttca gcctctccgc ggagcgcgcg acgacggcgc agacgggcgc cgacgcgcgc
      541 gaacggacgc tcgtgaacta cgcgcaccac atggcgcaca gctggatccc caagcgcgcg
      601 tacggcgccg gctactcccc gcacacgtgg gaggcgccgc ccgtgatcga cacgatctgg
      661 ttcaacgaag gcttcggccg gtatgcggcc gtccaggctc tcgcggacgg gatgccgccc
      721 gccgagagcg ccgcgttccg gacgcgaacg ctcgcgcgcc tgagggcgat tctcaaggaa
      781 gcgcccccgt tcgtccgccg gatgccgctc gcggagctct cgcgatccgc ctcgttcctg
      841 tactcggcgg acttccggat cgggatgagc acgttcgctc gcggagcgct catggcggcc
      901 gagatggacg accggatccg cgccgccacg ggcggagaga agagccttcg cgacggcctc
      961 cgggcgctcc tcaaggcccc gcagccgttc cggaccgaag aacttcccgt aatcttccgg
     1021 ggctccacgg gcgtggacgt tcgtgagatc ctcgatcgct ggatgaagga gacgacacca
     1081 tgaaacgcac tctcgccctg ctcgtcggcg cggtcctcgc ggccgcgggc tgctcgagcc
     1141 tctcgatctc gacggactac gacaaggccg ccgacttctc ctcgtacaag acgttcacgt
     1201 ggaaggacac cggcgagctc agggactcgc tctggagcaa gcgcttcgag ggagtcttct
     1261 cggacacgct cgcgtcgaaa ggcctcagga gggtcgactc cggcggcgac ctctgggtcg
     1321 cggcgcaccc gcgcctctcg aaggagacgc agatcaacac gtacaacacc ggctggggct
     1381 acggctacgg ctggtacggc tacggcggca tggggatggc gacgacgacc gtgaccgaga
     1441 tccccgtcgg gacgctcatg atcgacctcg tggacgcgaa gaagaaggag ctcgtgtggc
     1501 gcggcgtcgc gagcgacacg ctgaacacgg acccgaaccg caccgccgag gaccgcgaga
     1561 agaagctgcg cgaggtggcg gcgaagatgt tcgagggcta cccgccgaag aagtaggaaa
     1621 gaaaggaaaa gggataatca cgacatgaag acgatcacgg gattcctcct cctcgccctc
     1681 gcgggctccg cctccgccgc cgagaccttc acggccacgg ccgaggccaa gaacgccgcc
     1741 ggcgcctcga agagcgcgcc gatcacgatc acctactcgg ccctgtccac gccggccgaa
     1801 agggacgccc tctttgccgc gctcaagagc gggggccacg aagccgccaa gaaagtcctc
     1861 gccggcatga aggacatcgg caccatcgaa ggccccaaga agaaagtcgc cgtgaagtac
     1921 gcgttcgcgc gccccgtggg ctccggccgc atggtgacgg tcatcgccgc ggagccgatg
     1981 aacgcaatca agtcggacct ctcgaccacg ccgcccaagc cggggttcga cctaacgatc
     2041 gccctcctcg tcctcgatgc cggggacaag ggcagcggcg agttcacccc cgccgcgaag
     2101 atcaagctgc gcgaggacgg ggccctcacg acggaagact cgtccaccga gacgatctgg
     2161 ctgaagggcg tcgcgaagaa gtagcgcaca ggggagaaaa gggccgcgcc gggggaccgg
     2221 cgcggccctt ggccgcaggg cgtcagttcg cgctcttctg cgctccgccc gtcacggact
     2281 ccatgacgcg ctcgagattg aagctgcccg gcttctgccg cggcggaaac tccttgaagc
     2341 tctggagcca ctgcgcgacg tagccccccg ccggcgcgat cgcgaacatg tgctccatga
     2401 accacctctg gtaacccatg gcgtgctcgg cctcggcccg ctcgaaaggg tccatccgga
     2461 ggttcgtgat gagcggtgcg cgcagagggg tgaacggctc ctgccacacg cggagacctt
     2521 cgcccttctg ctcgaggaac gtgatcttcc agttctccca ccggagcgcg gcgacctggc
     2581 cgtcgtccgt ccagtagatg aactcctttc gcggccacgc ggcctcgccc ctcagcgcgg
     2641 ggccgagatc gtatccgtcg agatggacct tgtaggtcgc gccgcccatc gtccggccct
     2701 tgaggaggtc gtctttgacc gtcttgtcgc ccgccgcggc ggcaaacgtc gtgagcatgt
     2761 cctcgtgcgc cgcgatctcg ttgatgacgg tgccgggctt gatgaccccc ggccaccgga
     2821 tcatcgccgg gacgcggtag ccgccctccc agttcgtgtt cttctcgccg cggaacatcg
     2881 tcgtgccgcc gtcgggccac gtgaacgtct cggcgccgtt gtccgtcgag tacatgacga
     2941 tcgtcgtgtc gtcgaggccc agatcgtgca gcttcgcgag gacctggccg acgtgcccgt
     3001 cgtgctcgac catcccgtcc gcgtagacgc cgaggcccgt cttgccctcg gattccttct
     3061 tcaggtgcgt gaagatgtgc atgcgggtgg agttccacca gaggaagaag ggctttccgg
     3121 acttcttcgc gttctccatg aacgcgagcg ccttcgccgt gacctcctcg tcgatggtct
     3181 ccatgcgctt cctcgtgagc gggcccgtat cgctgatgcg gccgtccgcg aagctgtgga
     3241 tgacgccgcg cgggccgaac ttcttcttga actcggggtt cttcgggtaa tccgggttct
     3301 cgggctcttc ctccgcgttc aggtggtaga ggtttccgaa gaactcgtcg aacccgtggt
     3361 tcgtcgggag cgtagcgtcc gcatcgccga ggtggttctt gccgaactgc cccgtggcgt
     3421 acccttgcgc cttgaggacc gtcgcgatcg tcgggtcttc cttcttcatc ccttcgggcg
     3481 cgccggggag gccgaccttc gtgaggccgg tgcggatggg cgactggccc gtgacgaacg
     3541 cggcgcggcc cgccgtgcag ctctgctggc cgtaccagtc cgtgaagagc gccccgtcgg
     3601 cggcgatgcg gtcgatgttg ggcgtgcggt aacccatcat cccgcggttg tacgcgctga
     3661 tgttgaagcc gccgatgtcg tcgccccaga tgacgaggat gttcggcttt ccggcgggcg
     3721 cggccttcgg cgcggcggcc ggtttcgcgg cgggcgcagc cttcgccttg ggcgcctgcg
     3781 cgaacgcggc ggacgcggcg agcgtggagg agagaagagc gagggtcatg gacctacgaa
     3841 gggtcatcgg gcctcctttt gagatttcgg cagcagacgg gacggaacgt tgtttcctga
     3901 gacggtcccg tttccagaga ctgacctgag acatcgtttc atcggggcga tgcgccgtcc
     3961 aatgcatttg tcttgctaga atcatgcaat agatgcatcg tttgatgccg gacctccgcc
     4021 tcctcggaca cgccgtcgcg ctcgcggagc acgctcactt cgggaaggcg gccgagtccg
     4081 tgggcctgtc ccagcccgcg ttcagccggt ccatccaggc cctcgagaga catttcggct
     4141 gccggctctt cgagcgcgat cggggccggg cggtgccgac gccctacggg cggatcgccg
     4201 tcgagcgcgc ccgacagctc ctccacggcg cctccgagct ccagcgggag atcgggctcc
     4261 tgcgcggcgc cgacacgggc gagctgcgcg tctcggtggc gtcctacccc gctgagctgt
     4321 ccgtgcccgc cgcgctgcag cgtctcctgg cggcccaccc cgcgctcacc gtgcgggtcc
     4381 gggtccggga ctggcaggac ctcgtcaccg acgtccgggg cgagaccgtc gacctgggaa
     4441 tcggcgagat cagcgagctc gacggcgagg cccacctgga aaccgagctc gtcggcagtc
     4501 accgcgtctc cttcatctgc cggccgggac atccgctccg ccgaaaacgc cgggtgaccc
     4561 tgggcgacat ggtcgcgtac cctttcgcgg gcacggtctg gccgccgcgc gccgcgcgca
     4621 tgctgccgcc ccccccgttc gcggccgggg ccatcgacgc ggagacgggc cgtttcgtcc
     4681 cggcgatcaa cgtggaccgg cccctcaacg tgaaggacct cgtgcgcgag acgaacagcc
     4741 ttgcctacct tccccggacg ctgtgcgagg cggagctctt ctctggcacg ctcgccgtcg
     4801 tcccggtttc cgtgtcctgg gccaggctga actacgggtt catctggcgc cgcggccgga
     4861 tgcgttcccc cgcggcggtg gccttctccg ccgcggtccg cgccgccgag gcgggcgtcg
     4921 tcgcgcgcga ggccgatctc gcccaccgtt ttccgtgatg gcgccatccg cggacgcgag
     4981 actctccgtc gcttctggta tacggctcta gacatgagac gcctcctccg cgagccgctc
     5041 gtccatttcc tcgcgatcgg ggccgctctg tttctcgtct tcgcgtggaa gggcggcgcc
     5101 ggcgcctcgt ccggccggat cgtcgtgacg cgcgcgcggc tcgagtccct cgccgcgggc
     5161 ttcgcgcgca cgtggcagcg gccgccgacc gccgaggagc tgaagggcct cgtggacggt
     5221 tacgtccgcg aggagatcgc cgtgagggaa gccatggcga ccgggctgga tcgtgacgac
     5281 acgatcatcc gacggcgcct gaagcagaag gtcgacttcc tcgccgagga ccgcatcgac
     5341 gccgccccgc cgggcgaggc cgaactctcc gcgtggctga ccgcacaccc cgacctctac
     5401 cgcgccgaag agcgcgtctc cttccgacag gtctgcctga ctcccgagaa acgcggcggc
     5461 ccggccgccg cggcggccga ggcgaaacgc ctcgcggccg ctctcgagaa gaagggcccc
     5521 ggcgccgagg ccgcgggaga cagccttctc cttccccccg aaatgccgct cgcaccgaag
     5581 ggcgagattg cgcgcgtctt cgggagcgag ttcgccgacg ccgtcgtcaa actcgagccg
     5641 gggcgctgga ccgcgcccgt cccgtcgggc ttcggcgtcc acgccgtcct cgtcctcgag
     5701 agaaccgccg cccgcgcgcc cgcgctcgcg gacgtgcggt cggccgtcga gcgcgacttc
     5761 accgcggacc gcaggacgcg cgagctggac gccctctacg cgcgccttct cgcgaaaacg
     5821 aaggtcgtga tcgaaacggc gccggagaag aagtgagggc gcgcggcggc ctcggcctcg
     5881 tcctcctcgc cgcgctggcg gcggccccgc ccgccctcgc ccacgagaac cgccccgggt
     5941 tcctcgagat ggaggagacg tcgcccggcg tctacgcgtt cctgtggaag gcgccgttcg
     6001 gcggcgagac ggagatccac atcgcgcccg tccttccgcc gggctgcgtc ctcgcgacct
     6061 ccggcgggtc gcagctgacg acgtcggccg tcctcgtgcg cggcacgctg cgctgcgagg
     6121 gcggcctcgc gggaaagagg atcgtgatcg acggcctctc gtccacggcg accgacgttc
     6181 tcgtgcgcgt gcaccgcgcg gacggtgtgc tcgaaagcca cctcgtcggc ccggcggcgc
     6241 cgtccgtcac gctcgggagc gcgacgaccg gcgcccagcg cgcggcgtcg tacctgaagc
     6301 tcggcgtcga gcacatcctc atgggcgtcg atcacctgct cttcgtcctc gggctccttc
     6361 tcatcgtctc ggaccgctgg atgctcgtga agacgatcac gtccttcacg gtcgcccaca
     6421 gcctcacgct cgcggtcgcg accctcggct tcgcgtcggc gccgctcccg ccgctcaacg
     6481 cggcgatcgc gctctcgatc ctgttcctcg gcccggagat cgtgaggacg tggcgcggcg
     6541 agacgagctt cacgatccgc cacccgtggg tcgtcgcgtt cgcgttcggc ctcctgcatg
     6601 ggttcggttt cgcgagcggc ctgacggcca tgggcctccc gaaggcggag atcccgctcg
     6661 ccctcctcct cttcaacgtc ggcgtcgaga tcgggcagat gttcttcgtc gccgtgatcc
     6721 ttctcctcac acggtccttc tcgatcctcg agatccgctg gccgcgctgg gccgaggccc
     6781 tgccgggata cgccgtcggc tccctcggcg cgtactggac gattcagcgg acggtcatgc
     6841 tgctcggagg ggcgcggtga aggcgacgcg cgccttccgc gggtcctcgc ccgaggcgct
     6901 tctcgcgctc ctcgtcctcg tgctcgcggt gtcgcccgcg agggcacaca tccaggccgg
     6961 cgaggcggcg ggtttcctct ccggcttccg ccacccggtc tcgggcctcg atcacgtcct
     7021 cgcgatggtg tccgtcggac tctggggtgc gcagctcggc gcgcccgccg tctggctcct
     7081 gcccgtggtg ttcccgatgg tcatggcgtt cggcgggttc ctcggcctcg cgggcgtgcc
     7141 gctgccgggc gtcgagatcg cgatcgcgct ctcggccctc ctcctcggcc tcgccgtcgc
     7201 gcgggaggcc cgtccgcccc tcgcgctcgc cgcggcgctc gtcggcttct tcgcgatctt
     7261 ccacggacac gcgcacggca ccgagctccc gcccgggcag agcgggatcc tctacagcat
     7321 cggattcgtc gtggcgaccg gcctcctgca cctctcgggg atcgccatcg gcctgatcca
     7381 ccgctggagc tgggggcggg tcgcgctgcg tgtcgcggga tcgctggtgg cgatcgccgg
     7441 tttcggattt ctctggaagg cgctccgatg aggaggcgcg tgccgggcgc aggaatactg
     7501 gcgttcttcg ccgctctctt gatttcgggg cgcgccgagg cgcatctcgt cacgacaggc
     7561 ctcggccctg tctacgacgg aatcggccat ctcgtgctga cgcccgagga tctcgtgccc
     7621 atcgtcgcgc tggcgctcta cgccgggctc cggggcgcgc gcgcgggccg cctcgcgctc
     7681 ttcgtcctgc cggtcgcgtg gctcgtcggc ggcctcctcg gcctgaggtt cggcggcgcc
     7741 gcatcggccg ccgcgccgta tccggcgctc tcgttcatcc tcctcggcct cctcgtcgcg
     7801 gccgacgcga ggctccccga tgcggcagtc gcggcgctgg ccacagcgct cgggctcgtc
     7861 cacggttttc tgaacggccc cgcgctcgcg ggcaccggcc ccggcgccct cggcctcgtc
     7921 ggcatcgcgg ccgtcgcgtt cgtgatgatc gcgctcgtgt ctgctctcgt cgtgtctctg
     7981 aaggcccccg cggcgcgcat cgccgtgcgc gtggccggga gctggatcgg cgcgatcggt
     8041 cttctgcttc tcggctgggc gctgcgcacg cgcggcgtgc cccccgggcc cggtatgctc
     8101 gctccatgag caacgtggcg ggagccctcc tgacgttcgc cggtttcttc ctcgcgacac
     8161 tcgccttcat cgaagcgggc ctgcgggccg ggcggcgcgc cctcgccgga cccgattcga
     8221 gccggcccgc cggcctcggc acggtcgaga ccgtcgcctt cggtctcctc gggctcctcc
     8281 tcgcgttcac gttctcgggc gccgcatcgc ggttcgaagc ccgccgggcg ctcatcgtcg
     8341 aggaggcaaa cgacatcggg accgcgtggc tgaggctcga cgtcctgccg cccgccttcc
     8401 agccgccact gcgcgacgcg ttccggcgct acacggactc ccgcatcctc acctacgcga
     8461 aggtcgccgc gggggacatc gcgggcggcc gagccgagta cgcccgcggc gtcgcgatcc
     8521 agaacgagat ctgggcgggc gcggtggctg cgtgccgcgc ggccccctcg caggccacga
     8581 tcgtcctctt cccggccctc aacgcgatga tcgacatcac gacgacgcgg atggccgcga
     8641 ccgacatgca cccgcccaag atcatctact tcgtcctcgg cgggatctcc ctcgtctgcg
     8701 cactcctcgt cgggtacgag atgggcgcga gcaccgcgcg gagctggctc cacgtcgtcg
     8761 ggttcgcggc catcctcgcc ttcaccctgt acgtgatcct ggacttcgaa tatccgcgga
     8821 tcggcctcat ccgggtcgaa gacttcgaca agtacctcgt ccaggtgcgc gcgtcgatga
     8881 aatgaagtcg ttttccgcgc gcgcgtggat cgccgtcctc gcgctcctcg ccgcggcgcg
     8941 gttcgcgacg atggcgacgg acgcgtggca ctgggacgag gtgctgctcg ccgacgccgt
     9001 cgcgcacgga atcgacctcc gcgtccaccg cccgcacccg cccggctacc cgctgctcgt
     9061 ggagacggcc gctctcgtcc acgggacggg cgtcgacccg taccgttctc tcgcgctcgt
     9121 gggaacggcc gggggactcc ttgcggcggc cgcgctcgcg gcgttcctcg ccgccgccgg
     9181 cctcggcgcc gacttcgcat gcctcggcgg actcctctac gccgtcgtgc cgtcggtttg
     9241 gctcttcggc gtgcgcggat tcagcgacgc ccccgccgcg gccgccgtct ttgcgtcggc
     9301 cgccctcctc ctctccgcgg cggagcggcg cttgcccggc cgcgccgccg ccgggttcg
//
LOCUS       DAOMFY010000140         9337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2245_length_9337_cov_9.520254, whole genome shotgun sequence.
ACCESSION   DAOMFY010000140 DAOMFY010000000
VERSION     DAOMFY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_9337_cov_9.520254"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="PLB01_11830"
     CDS             complement(<1..1790)
                     /locus_tag="PLB01_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQQ78035.1"
                     /translation="MRRAFSLLCGALLLTAAASPAQAAKPAPSAARPPRVIVVGWDGA
                     DWSLLDPLLASGRLPHLKSLLEKGAQARLETYRPRASPLLWTTMATGLTPPEHGVVDF
                     QEFDVATGSSLPVSGRSRTGPAIWNVASAKGLTSGVVGWWATWPAEAVKGFFISDRAS
                     PVLFDAATLSQSPGLTWPPELADGVRLLGRREGSPSYEDVAKFLRVSRAEFDASVAAG
                     RDLDDVVTGFRKILGSTRVYARTALDLYDRSKPDLLMLYLEGTDEIGHVLGKYNPPLL
                     PGVDRGEFERYKDAVALFYAECDRILGELAARAEKDGATLMLVSDHGFKWGEDRPVLV
                     SSVRAETAYLWHEPWGVLVLSGPGAARSKERRTVNIFDVAPILCRLLGLPPDVRMKGR
                     VPAGLLSSGAPAPKKAVAWDKAFKVERAPITRNLAAEKKAGEEFAKKLVSLGYLSGSS
                     AQAPVAADSGTGDVRMTPYGVSNIGVFYAESGRVKESVPWFEKAIAADPKAPTLRTNL
                     ADAWEKLGRMDAADAEALKALELGGEDANDLLLARVVKRMESGNAPAAIVLLEKATRQ
                     VRPARPQLQDALGRLYFDRRRCAEAQRVFE"
     gene            complement(1848..4874)
                     /locus_tag="PLB01_11835"
     CDS             complement(1848..4874)
                     /locus_tag="PLB01_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ78036.1"
                     /translation="MKNRWLSILVVLALAVVGLTANVALAQTTGDIDGTVTDSNGAPL
                     PGASVTISSPALQGTRTAVTDAAGRFRFPALPGGTYTVTGALSGFTKSERRNVVVAIG
                     RVASVPLTMSVSVKEEVVVTGEAPVIDTAKTTIGVTGTSEQISRLPLARNFTSVASTA
                     PGTGTDNSGGITFYGATGLENQYIIDGVNTTGIKIGNVGKTLTNEFVQEVEVKTGGYE
                     AEFGRALGGTINVVTKSGGNEFHGDLFGYYDSDNLAASNAHVDDRTAVGQSQLILPKR
                     MDFGADLGGYFVKDRLWFFGAVNGVRRDQDYIRNTSIYYTPTGGLDTSKGTGGFATSS
                     GTDETRTTLYSGKLTFRLGEAHTLSASVFGDPGKFTGRQVTTIGPDSGVIWDTENGGL
                     DIAAKYDGIFGTSFLLSGQYSHHEEKNENSSPFENTLSNLQNRGGRTQIVSGGPTYFV
                     DESYKRDDFKLVGSFFAGAHEIKVGGEYEKLSSSFSEKYPGGARIFQFMTSAGAFNYA
                     YQRYFAKVPLNCIAKYDASGNLQTGNFGPPANAAAANACAGYQTAASVDNPPTTKNLA
                     IFAQDSWKILKNLTLNVGIRYEEQNLLDADGNSAIKINGEWSPRVGIIWDPMSNGRSK
                     VYASYGRYYSTIPQDIQTRALGNEYTVFAYNYTRDKVDPITDYAFFGYQTIQGGELTQ
                     PDLKGMYQDEIIGGIEYEVFKGWSFGVKGIYKKLGRVIEDRCDLLDTRVGLASYVPPS
                     ALTTCALVNPGDDSPLQVIKDPTNPACQGPAGSTVLSGNCPSINPSRYYRGLELTATH
                     RFSNNFYLLASYIYSQLNGNYSGNFSQTRENGQSDPNINADFDYIDLTPNNYGLLRNN
                     RKNQFKLTGTYAFNFGLVTSINAVYADGRPMSVRGYARPGYSQERYYMARGSFDTLPS
                     TYNIDLHLEYGFRLGAVTITPLLDVFNLTDFQGVTSRDEVFCTSTGTCNAVLASTGKQ
                     RKEGPYTTANSSNPNFNRDIAWQNPRLLRLGLRVSF"
     gene            complement(5212..6912)
                     /locus_tag="PLB01_11840"
     CDS             complement(5212..6912)
                     /locus_tag="PLB01_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HQQ78037.1"
                     /translation="MTIRLPKDAGERTLLVLLLLATFLLVLEPGRVPLFEPDEGRYGE
                     IPREMLATDDWVTPRLNGVLYFEKPPLYYWSVAASMAVLGPTELAVRLPGKLSAAGMV
                     LLAAAFARRRYGARTGLLAGLVCASSLLVVALARIAIIDPMLSLAMSGAAFAFAAFAE
                     GDAGGDAKRARRALYGFHVACAAAVLLKGLIGVVLPGGAIVFWTLWTGRWRTLARVFS
                     PGPLLVFLALAVPWHVAMARRHPDFLQFYFVHEHFDRFAKTEHKRTGPLVYFVPVLLA
                     GFLPWTAFLGRWKETWPGLSRAAWKARATEGFLWMFSGLVFVFFSVSRSKLIPYVLPI
                     WPALAVLLALGIERARARGASFRGERIATGALFGALLCVGAAYGFGAGYLGRFGAAAA
                     GACALLALFGGFVLNVSPRLTAGADAACLVAAPWLAFIAFLLAVLPGAAHWVTPWPVV
                     APALAALDGDAVLVQRGHYLEVLPFYAARTTPVAALGWSELDFGRSHAGTEALFPSEE
                     AFAALWNGPRKVVVMVHRDHLAAFAKPPLADTPATILGRENNAKHMALANRITESQSK
                     "
     gene            complement(6909..8477)
                     /locus_tag="PLB01_11845"
     CDS             complement(6909..8477)
                     /locus_tag="PLB01_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HQQ78038.1"
                     /translation="MARAGALGDPRPDLVWGVSPRVCVLRPAARLLPGGRLSAPAAVV
                     AKGLSKSYRKWGRTRAFGTLKSALLGRGFGAALAPSETVQALSDVSFAVQRGETFGVV
                     GPNGCGKSTLLKLVAGLFKPTAGTLEVTGKVSALIELGAGFHPEISGRENVVINGVML
                     GLTRREIERKLPEIVAFSGLEEFIDEPVKTYSSGMYVRLGFAVAVHVDPDVLVVDEVL
                     AVGDEAFAHRCLDTIADFSRRGKTIFFVSHSLALVEELCDRVLYLDHGKTKALGDPRE
                     TLARYRMDVAAEEGARLARAQGENLAALRAEGIEEERREEKIFLEGGESKAPAGTAPA
                     VVAPQPNSPFEEKPPEPAARVPQATTPPAPEPLEQKESPPRRWGSREVEITGCRMLDA
                     SGVERYAFRSGESVTVEIAVAPRAPLSDFVFGIGLFTPEDVCVHGTNTELDGFLPSKL
                     GGPAVARVTLPALDLGTGTYLVDVAVHSRREAPYDYWRGACRFQVDAPDRSAGIYRPV
                     RRWSFEGPIALERK"
     gene            complement(8366..9175)
                     /locus_tag="PLB01_11850"
     CDS             complement(8366..9175)
                     /locus_tag="PLB01_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78039.1"
                     /translation="MLKNLAGLWRHRALVGVLVSRELKARYRGSVLGFFWSLLNPLLM
                     LAVYSVVFAFIFPGRSASTSPYALFLFTGLLPWNWLAGGLTDAASSLTTHGALLKKIL
                     FPAEVLPLVAVLAQGIHFLLALPVLAVGLGLGAFGVFGAPVPLGLPVAQAVPLFFLEG
                     VFLAGLGLFLAALTVHFRDVKDILSTALSLWFFATPVLYVIGDIRSERLRAALLWNPA
                     TPFFEAWRDAFFRAAWLAPERWGILVLISSGAFLLGYAFFDRLRDSFPEAV"
BASE COUNT         1519 a         3062 c         3374 g         1382 t
ORIGIN      
        1 tcctcgaaca cgcgctgcgc ctccgcgcag cgccggcggt cgaagtagag gcggccgagc
       61 gcgtcctgga gctgcggccg cgcgggccgc acctgccgcg tcgccttctc gagaaggacg
      121 atcgccgcgg gcgcgttccc cgattccatc cgcttcacga cgcgggcgag gagcaggtcg
      181 ttcgcgtcct ctcccccgag ctcgagggcc ttcagggcct cggcgtcggc ggcgtccatc
      241 cggcccagtt tctcccaggc gtccgcgagg ttcgtccgga gcgtgggcgc cttcgggtcc
      301 gcggcgattg ccttctcgaa ccaggggacg gactccttga cgcgcccgct ctcggcgtag
      361 aagacgccga tgttggacac gccgtacggc gtcatccgca cgtcgcccgt gccggagtcc
      421 gcggcgacgg gcgcctgagc cgaggagccc gagaggtagc cgagggagac gagcttcttc
      481 gcgaactcct cgcccgcctt cttctcggcg gcgaggttgc gcgtgatcgg ggcgcgttcg
      541 accttgaacg ccttgtccca cgccaccgcc ttcttcggcg caggcgcccc ggaggacagg
      601 agccccgccg gaacgcggcc cttcatccgg acgtcgggcg gcaggccgag aagccggcag
      661 aggatcggcg cgacgtcgaa tatgttgacc gtccgccgct ccttcgagcg cgcggccccc
      721 gggccagaga ggacgaggac gccccacggc tcgtgccaga ggtacgccgt ctcggcccgg
      781 accgacgaca cgagcaccgg cctgtcctcg ccccacttga agccgtggtc cgagacgagc
      841 atgagcgtcg cgccgtcttt ctccgcgcgc gcggccagct cgccgaggat ccggtcgcac
      901 tctgcgtaga agagggccac cgcgtccttg tacctctcga actcgccccg gtcgacgccc
      961 gggaggagcg gcggattgta tttcccgagg acgtgcccga tctcgtccgt gccctcgagg
     1021 tagagcatga gaaggtcggg cttgctgcgg tcgtagaggt cgagcgcggt cctcgcgtag
     1081 acgcgcgtcg agccgaggat cttccggaag ccggtcacga cgtcgtcgag gtcgcggccg
     1141 gcggccacgg acgcgtcgaa ctccgcgcgc gagacgcgga ggaacttcgc gacgtcctcg
     1201 tacgaggggc tgccctcccg gcggccgagg aggcggacgc cgtccgcgag ctcgggcggc
     1261 cacgtgaggc cgggcgactg cgagagcgtc gccgcgtcga agagaaccgg cgacgcccgg
     1321 tcggagatga agaacccctt gaccgcctcg gcgggccacg tcgcccacca gccgacgacg
     1381 cccgaggtca gccccttcgc ggaggccacg ttccagatcg cgggccccgt gcgcgaacga
     1441 cccgagacgg gcagggacga tccggtcgcg acgtcgaatt cctggaagtc cacgacgccg
     1501 tgctcgggcg gcgtgaggcc cgtggccatc gtcgtccaga ggagcggcga ggcgcggggc
     1561 cggtacgtct cgagccgggc ctgcgcgccc ttctcgagaa gggacttcag gtgcgggagc
     1621 cgcccggacg cgagcagcgg gtcgaggagg ctccagtcgg cgccgtccca gcccacgacg
     1681 atgacgcggg gcgggcgcgc ggcggagggc gcgggtttcg cggcctgcgc gggcgacgcg
     1741 gcggccgtga ggagcagggc cccacagagg agggaaaagg cgcgtctcat ggcgaaaaaa
     1801 aaggggaagc ctttcggctt cccctcgagt ctaaccggga aagaactcta gaacgagacg
     1861 cggagcccga ggcggagcag ccgcgggttc tgccaggcga tgtcgcggtt gaagttcggg
     1921 ttggaggagt tcgccgtcgt gtacgggcct tccttgcgct gcttgccggt cgaggcgagc
     1981 accgcgttgc acgtgccggt cgacgtgcag aagacctcgt cgcgcgacgt gacgccctgg
     2041 aagtccgtca ggttgaacac gtcgaggagc ggggtgatcg tgacggcgcc caggcggaag
     2101 ccgtactcga ggtgaaggtc gatgttgtac gtcgacggca gcgtgtcgaa gctgccgcgc
     2161 gccatgtagt agcgctcctg ggagtaaccg gggcgcgcgt agccgcgcac cgacatcggg
     2221 cggccgtccg cgtacacggc gttgatcgac gtcacgaggc cgaagttgaa cgcgtacgtg
     2281 cccgtcagct tgaactggtt cttccggttg ttgcggagga ggccgtagtt gttcggggtg
     2341 aggtcgatgt agtcgaagtc cgcgttgatg ttcgggtcgc tctggccgtt ctcgcgcgtc
     2401 tgcgagaagt tgcccgagta gttgccgttg agctgcgagt agatgtagct cgcgaggagg
     2461 tagaagttgt tcgagaagcg gtgggtcgcc gtcagctcga ggccgcggta gtagcggctc
     2521 gggttgatcg acgggcagtt gcccgagagc accgtggagc ccgcggggcc ctggcaggcc
     2581 gggttcgtcg ggtccttgat gacctggagc gggctgtcgt cgcccgggtt gacgagcgcg
     2641 cacgtcgtga gcgccgacgg ggggacgtag gaggcgaggc cgacgcgggt gtcgagaagg
     2701 tcgcagcggt cttcgatgac gcgcccgagc ttcttgtaga tgcccttgac gccgaaggac
     2761 cagcccttga agacctcgta ctcgatgccg ccgatgatct cgtcctggta catgcccttc
     2821 aggtcgggct gcgtgagctc gccgccctga atggtctggt acccgaagaa ggcgtagtcg
     2881 gtgatcgggt ccaccttgtc gcgcgtgtag ttgtacgcga acacggtgta ctcgttgccg
     2941 agggcgcggg tctggatgtc ctgggggatc gtggagtagt agcggccgta ggaagcgtag
     3001 accttcgagc ggccgttgct catcggatcc cagatgatgc cgacgcgcgg ggaccactcg
     3061 ccgttgatct tgatggccga gttgccgtcc gcgtcgagga ggttctgctc ctcgtaccgg
     3121 atgcccacgt tgagggtgag gttcttcagg atcttccacg agtcctgcgc gaagatcgcg
     3181 aggttcttcg tggtgggcgg gttgtcgacc gacgccgccg tctggtagcc ggcgcaggcg
     3241 ttcgccgccg cggcgttcgc cggcgggccg aagttgcccg tctggaggtt gccggaggcg
     3301 tcgtacttcg cgatgcagtt caggggaacc ttcgcgaagt agcgctggta ggcgtagttg
     3361 aacgcgccgg cgctcgtcat gaactggaag atgcgcgcgc cgccggggta cttctccgag
     3421 aacgacgacg agagcttctc gtactcgccg ccgaccttga tctcgtgcgc gcccgcgaag
     3481 aacgagccca cgagcttgaa gtcgtcgcgc ttgtacgatt cgtccacgaa atacgtcggg
     3541 ccgcccgaga cgatctgcgt ccggccgccg cggttctgga gattcgagag cgtgttctcg
     3601 aacggcgagg agttctcgtt cttctcctcg tggtgcgagt actggcccga gaggaggaac
     3661 gacgtgccga agatgccgtc gtacttggcg gcgatgtcga ggccgccgtt ctccgtgtcc
     3721 cagatgacgc ccgagtccgg gccgatcgtc gtgacctggc ggcccgtgaa cttgccgggg
     3781 tcgccgaaga cggacgcgga gagcgtgtgg gcctcgccga ggcggaacgt gagcttgccc
     3841 gagtagagcg tcgtgcgcgt ctcgtccgtg ccgctcgacg tcgcgaagcc gccggtgccc
     3901 ttcgacgtgt cgaggccgcc cgtcggggtg tagtagatcg acgtgttgcg gatgtagtcc
     3961 tggtcgcgcc tcacgccgtt gacggcgccg aagaaccaga ggcggtcctt cacgaagtag
     4021 ccgccgaggt ccgcgccgaa gtccatgcgc ttcggcagga tgagctggct ctggccgacg
     4081 gccgtccggt cgtcgacgtg ggcgttcgag gccgcgaggt tgtcgctgtc gtagtagccg
     4141 aacaggtccc cgtggaactc gttgccgccc gacttcgtca cgacgttgat cgtgccgccg
     4201 agagcgcggc cgaactcggc ctcgtagccg ccggtcttga cttcgacttc ctggacgaac
     4261 tcgttcgtga gcgtcttgcc gacgttgccg atcttgatgc cggtcgtgtt gacgccgtcg
     4321 atgatgtact ggttctcgag gccggtcgcc ccgtagaacg tgatgccgcc cgagttgtcg
     4381 gtgcccgtgc cgggcgccgt cgacgcgacg gacgtgaagt tgcgggcgag cgggagccgc
     4441 gagatctgct ccgacgtgcc cgtgacgccg atcgtcgtct tggccgtgtc gatgaccgga
     4501 gcctcacccg tgacgacgac ctcttccttg acggaaacgg acatcgtgag cgggaccgag
     4561 gccacgcggc cgatcgccac gacgacgttg cggcgctcgg acttcgtgaa gcccgagagc
     4621 gcgcccgtca ccgtgtacgt gccgcccggc agcgccggga agcggaaacg gcccgccgcg
     4681 tccgtgacgg ccgtgcgggt gccctgcagg gccggcgacg agatcgtcac cgacgcgccg
     4741 gggagcggag cgccgttgga gtcggtgacc gtgccgtcga tgtcaccggt ggtctgtgcg
     4801 agagccacgt tcgccgtcag accgacgacg gcgagcgcga gcaccacgag gatgctcagc
     4861 cagcgatttt tcattctgtc tacctcctga gatttaaggg atcgatcgaa acttcgttaa
     4921 cggtttcgcg taatgaagac tgggaagaat tggagccggg ctcctccttt cgaagaaatt
     4981 cgttcggaag catctgagcc tgttgctttc tcgctcttct cgttccttct tcactcaacg
     5041 caggcgcttg agatcaatgc gtccgcactc cgccacacgg tgctgtgacc agacccacga
     5101 aaacgggtgc aaaggctctg ccgtcaagag tcgcaagcgt actcaatcta agcctttgca
     5161 gtcaaagggt gcggtttcgt cccagccccc gtggcccccg tcccgatcca attacttgga
     5221 ttgggattcc gtaatccggt tcgcgagtgc catgtgcttg gcgttgttct cgcggcccag
     5281 gatggtggcg ggcgtgtccg caagaggcgg tttcgcgaac gcggcgaggt ggtcccggtg
     5341 gaccatgacg acgacctttc gaggcccgtt ccagagggcc gcgaacgcct cctccgaagg
     5401 aaagagcgcc tcggtgcccg cgtgggagcg cccgaagtcg agctcgctcc agcccagcgc
     5461 cgcgaccggc gtcgtccgcg cggcgtagaa cggcagcacc tcgaggtagt ggccgcgctg
     5521 gacgaggacg gcgtcgccgt caagggccgc gagagccggc gcaacgaccg gccacggcgt
     5581 cacccagtgc gccgcgcccg gcaggacggc gaggaggaac gcgatgaacg cgagccacgg
     5641 cgcggcaacg aggcaggccg cgtcggcccc ggccgtcagg cgcggcgaga cgttcaggac
     5701 gaagccgccg aagagggcga ggagcgcgca cgcgcctgcc gccgccgcgc cgaagcgccc
     5761 gaggtagccc gcgccgaagc cataggcggc gccgacgcag agaagcgcgc cgaagagagc
     5821 tcctgtcgcg atgcgctccc cccggaacga ggcgcctcga gcgcgcgccc gctcgatccc
     5881 gagcgcgagg aggaccgcga gcgccggcca gatcgggagc acgtacggga tgagcttcga
     5941 ccgcgacacg gagaaaaaga cgaagacgag gcccgagaac atccagagga agccctccgt
     6001 ggcgcgcgcc ttccacgccg cgcgcgagag gcccggccac gtctccttcc atcgcccgag
     6061 gaaggcggtc cacgggagga accccgcgag gaggaccggc acgaagtaga cgagcgggcc
     6121 cgtgcgcttg tgctcggtct tcgcgaatcg gtcgaagtgc tcgtggacga agtagaactg
     6181 gaggaagtcc gggtgccgcc gggccatcgc gacgtgccac ggcacggcga gcgcaaggaa
     6241 gacgaggagc ggccccggcg agaagacgcg cgcgagcgtc cgccagcggc cggtccagag
     6301 cgtccagaac acgatcgcgc cgcccggcag gacgacgccg atgagcccct tcaggaggac
     6361 cgccgcggcg cacgcgacgt ggaagccgta gagcgcccgc cgcgcgcgct ttgcgtcgcc
     6421 gcccgcgtct ccctccgcga acgccgcgaa ggcgaacgcc gcgcctgaca tcgcgagcga
     6481 cagcatcggg tcgatgatcg cgatgcgcgc gagggcgacg acgaggagcg acgacgcgca
     6541 gacgaggccc gcgaggaggc cggtccgcgc cccgtaccgg cgccgcgcga acgcggccgc
     6601 gaggaggacc atccccgccg ccgagagctt gccgggcagg cgcacggcga gctccgtcgg
     6661 gccgaggacc gccatcgagg ccgcgaccga ccagtagtag agaggcggct tctcgaagta
     6721 gaggacgccg ttgaggcgcg gcgtcaccca gtcgtccgtc gcgagcatct cgcgcgggat
     6781 ctcgccgtag cggccctcgt ccggctcgaa gagcggcacg cgccccggct cgaggacgag
     6841 aaggaacgtg gcgagaagga ggaggacgag gagcgtcctc tcccccgcgt ccttcggcag
     6901 tcggatcgtc atttcctctc cagcgcgatc ggcccttcga aagaccaacg gcggaccggc
     6961 cggtagattc cggcgctccg gtcgggcgca tccacctgga agcggcacgc gccgcgccag
     7021 tagtcgtacg gggcctcgcg gcgcgagtgg accgcgacgt cgacgaggta cgttcccgtc
     7081 ccgagatcga gagcgggcag cgtcacgcgc gcgacggccg ggccgccgag cttcgacggc
     7141 aggaacccgt ccagctccgt gttcgtcccg tgcacgcaga cgtcctcggg cgtgaagaga
     7201 ccgatgccga agacgaaatc cgagagaggc gcgcgcggcg cgacggcgat ctcgacggtg
     7261 acgctctcgc cggagcggaa cgcgtaccgc tcgacgccgg aggcgtcgag catccggcag
     7321 cccgtgatct cgacctcccg gctcccccag cggcggggag gagattcctt ctgttccaga
     7381 ggttcaggcg cgggcggagt cgtcgcctgt ggcacgcgag ctgcgggttc cggtggtttt
     7441 tcttcgaagg gtgagttggg ctggggcgcg accacggccg gtgcggtgcc ggcaggcgcc
     7501 ttgctctcac caccttctaa gaaaatcttc tcttctcttc tctcctcttc gatcccctcg
     7561 gcgcgaagcg ccgcaagatt ttctccctgg gcgcgcgcga ggcgcgcgcc ctcttctgcc
     7621 gcgacgtcca tgcggtagcg cgcgagcgtt tcccgcgggt cgccgagcgc cttcgtcttt
     7681 ccgtggtcga ggtagaggac gcggtcgcag agctcctcga cgagcgcgag cgagtgcgac
     7741 acgaagaaga tcgtcttgcc gcgccgcgag aagtccgcga tcgtgtcgag gcagcggtgc
     7801 gcgaacgcct cgtcgccgac ggcgaggacc tcgtccacga cgaggacgtc cgggtccacg
     7861 tgcacggcca ccgcgaaccc gaggcgcacg tacatgcccg aggagtaggt cttgacgggc
     7921 tcgtcgatga actcctcgag ccccgagaac gcgacgatct ccgggagctt cctctcgatc
     7981 tcccggcgcg tgaggccgag catgacgccg ttgatcacga cgttctcgcg gccggagatt
     8041 tccgggtgga agcccgcgcc gagctcgatg agcgccgaga ccttgcccgt cacctcgagc
     8101 gtgcccgccg tcggcttgaa gaggcccgcg acgagcttca ggagcgtgga cttgccgcag
     8161 ccgttggggc ccacgacgcc gaacgtttca ccccgctgca cggcgaacga cacgtccgac
     8221 agcgcctgga cggtctccga cggggcgagc gcggcgccga agccccggcc gaggagcgcc
     8281 gacttgagcg tgccgaacgc gcgcgtccgg ccccacttcc ggtaggactt cgagagcccc
     8341 ttcgcgacga cggccgcggg cgcgctcaga cggcctccgg gaaggagtcg cgcagccggt
     8401 cgaagaacgc atacccgagg agaaacgccc cagacgagat caggacgagg atcccccagc
     8461 gctccggcgc gagccaggcg gcccggaaga acgcgtcgcg ccacgcctcg aagaacggcg
     8521 tcgccggatt ccacaggagc gccgcgcgca ggcgctcgga acggatgtcg ccgatcacgt
     8581 agaggacggg cgtcgcgaaa aaccagaggg aaagcgcggt ggagagaatg tccttgacgt
     8641 cgcgaaaatg caccgtgaga gccgcgagaa acagaccgag ccccgcgagg aatacacctt
     8701 ccaggaaaaa cagcggcacg gcctgcgcga ccggaagccc gaggggaacg ggcgctccaa
     8761 agacgccgaa cgctccgaga ccgagcccga cggcgaggac gggcagcgcg aggaggaagt
     8821 ggatcccctg cgcgaggacg gcgacgagcg gcagcacctc ggccgggaag aggatcttct
     8881 tgagaagcgc gccgtgcgtc gtgagagacg acgccgcgtc cgtcaggccg cccgcgagcc
     8941 agttccaggg caggaggccc gtgaagagga agagggcgta cgggctcgtc gaggcgctcc
     9001 gcccggggaa gatgaacgcg aagacgaccg agtagaccgc gagcatgagg agcgggttca
     9061 ggagcgacca gaagaacccg aggacgcttc cccggtaccg cgccttgagc tccctcgaga
     9121 cgaggacgcc gaccagcgcc cgatggcgcc agaggccggc caggttcttc agcacgtcgc
     9181 gtccatcccg aaggaccgat gatacgtagg atgggcgccg tgggagcccg cgtcgccgcc
     9241 gagtccgtct ccaagtccta cctgccgggg ccgggcgcgg cacccgtgga ggcgctcgcg
     9301 gacgtctcgc tcgagcttcc cgccgggagc ttcaccg
//
LOCUS       DAOMFY010000141         9313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2253_length_9313_cov_6.827825, whole genome shotgun sequence.
ACCESSION   DAOMFY010000141 DAOMFY010000000
VERSION     DAOMFY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2253_length_9313_cov_6.827825"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLB01_11855"
     CDS             <1..526
                     /locus_tag="PLB01_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQQ78040.1"
                     /translation="HAERVRDLALALFDQTHALHQLTALEREWLEHAALLHDLGLSVA
                     YARHHHHSHYLIRHGDLKGFTQDEVEVIALVARYHRKAPPDPSHEAFRTLDPWKKPVV
                     EKLSALLRVADALDRTHRGLVTRVRVVLRGKKAHLTLGARSACDLELWALKKKGKLFE
                     KVFGTKIAARVVVG"
     gene            535..1077
                     /locus_tag="PLB01_11860"
     CDS             535..1077
                     /locus_tag="PLB01_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78041.1"
                     /translation="MSFPQATVLLFAAAAAATVTPTPTAAPAPAETPRPRSLADVARE
                     RKLVPLKGAGESPVAGTQGPLRVEEIQDNGAVADGFLSVYGRVRNTGRSPVCRVRLFL
                     RMFDENGVLLSKGETTTDLKVVPAGDAVPFGGRLKVPPGVRGSQERAPDVLAEGPART
                     NSQRVARVEGEVLDFSEECR"
     gene            1074..1727
                     /locus_tag="PLB01_11865"
     CDS             1074..1727
                     /locus_tag="PLB01_11865"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HQQ78042.1"
                     /translation="MSVLALGFVLLQAAAATPSPAPTPTPSPRPSAAGPRTLQDVARE
                     RKLAGAQKGKGSLVTISGGPSTETPAPAGTPAPAGTPEAGAATPEPPASTASVRVTEV
                     RNDGVVDPNGGVRVNGTVRNGGYKAACNVVITVRIMDNKGIYLASGEAAPDTAVIPPG
                     AVVSFHTIVQAPPGVRGARVDPDRRDVTDGSTTMAGDWKLLGGTEATVANATEDCGR"
     gene            complement(1737..2198)
                     /locus_tag="PLB01_11870"
     CDS             complement(1737..2198)
                     /locus_tag="PLB01_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78043.1"
                     /translation="MALFGKGGGASGGMKPGFREMKWGDQPTKQMEVLDELAEEKFCR
                     LEHDDLTWSDAPVDKIIYLFWSNRLSDVHIEIPPASADRILKDLNDGWGRPEQPNKFI
                     EDFLWKNKAHGPEATEALFSRNPNTRGATLTISSSYIKAKKALAKGKPPAR"
     gene            complement(2254..4380)
                     /locus_tag="PLB01_11875"
     CDS             complement(2254..4380)
                     /locus_tag="PLB01_11875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQQ78044.1"
                     /translation="MPLAFSRLAVAVIVLFAAVPAAAQAPPPPNPFFEEWTTPFGMPP
                     FERIKAEHFKPAFEAGIAERKKEVEAIAANPAAPTFANTVEALENGGLALAKVSDVFF
                     ALTGADTNDALQAINKEVAPKLSALRDDVLLNEKLFARVKAVWEAREKLPLTVEQKKL
                     LEETWKDFVRGGANLAPEQKKRFREINQELSVLGLKFADNLLKETNGFRLVVDRKEDL
                     AGLPSALVSAAADAAKAAKLEGKWAFTPQAPSILPFLTFAENRELRKKILDGYTSRGD
                     HGDALDNKTTLSRIAALRSERARLLGFKTHADFVLEENMAKTPAGVYGLLDRVWTPAL
                     AMAKRERADLQAAVKAGGKDFALEAWDWRYYSEKVRKARFDLDDQALRPYFPIDRVRE
                     GVFYAANRLYGLTFTERTDVPKYHPEVRVFEVKDADGSFLALYTVDYHPRPGKRSGAW
                     CGRIRRGWVREGKDVRPIVTNVTNFTRPAGDAPALLSLEEVRTLFHEFGHAVNGFVSK
                     VTYRSMGSTSRDFVELPSQIMESWALEPEVLKVYAKHWKTGESIPLDLVRKIEKAGTY
                     GEGFRTTEYLAASYLDMDWHTLPEPKEMEASAFEKASLGRIGLIPEIVVRYRSPYFQH
                     IFGPGGGYSAGYYSYIWSDVLVADAYDLFREKGIFDKATATSFRKNILEKGGTEDAMT
                     LYKRFRGREPIVEPLLVKRGLADPKP"
     gene            complement(4469..5377)
                     /locus_tag="PLB01_11880"
     CDS             complement(4469..5377)
                     /locus_tag="PLB01_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78045.1"
                     /translation="MDVAVPCGNEDSAADRLALVAQPPVSSSLRRVRRRVKPHLEYYG
                     GPVVSNAKVVAVRWNATVPADHSAAMEGFYRSLTKGAMFDWLSEYDTVGLTGFADGLS
                     GSDQRIGHGSFAGTYTMTPSQGATVTDSQITAALAAHVASGAIPPPEVDPQGNPVTVY
                     MIDFPDSVQIDDGAGLRSCVNFCAYHSSTRINGVRSAYGVLPAISGGCLTGCGAHPNP
                     FDIATSNASHELVEAVTDPAIGEIGATLTRPAAWFDPQPDQAEIADICASVADRDEML
                     DGYWVQREWSNRWRACIVAPPPGALP"
     gene            complement(5519..5595)
                     /locus_tag="PLB01_11885"
     tRNA            complement(5519..5595)
                     /locus_tag="PLB01_11885"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5559..5561),aa:Glu,seq:ttc)
     gene            complement(5637..5957)
                     /locus_tag="PLB01_11890"
     CDS             complement(5637..5957)
                     /locus_tag="PLB01_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78046.1"
                     /translation="MVISPAKRSLRLRASAEAFEVLRSAKSPRISNAELLKHSVRSDH
                     EPHPRVVREVHQGLKEAQAARPAGAPSVLKKISGVFSRTGVKAEPSVDDIVRRFEQAF
                     VQPD"
     gene            complement(6053..6129)
                     /locus_tag="PLB01_11895"
     tRNA            complement(6053..6129)
                     /locus_tag="PLB01_11895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6093..6095),aa:Lys,seq:ttt)
     gene            complement(6177..7511)
                     /locus_tag="PLB01_11900"
     CDS             complement(6177..7511)
                     /locus_tag="PLB01_11900"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQQ78047.1"
                     /translation="MKGSALAIVGGQWGDEGKGKVVDLISPSFDLVARYQGGHNAGHT
                     VKFGDRHFALRLIPSGICRPGVMNVVGNGLVIDPRAMLSEMDSLRAAGVRIEENLLIS
                     DRAHVILPTHALLDGAREKALGGANIGTTSRGIGPAYETKANRTGLRMADLVDPDRFR
                     ALARPVLTHHDAVLSHFYGVEPAPIEETLDTYVECGKKLARHVTDTSAWLNAQLAAGK
                     KLLCEGAQGVMLDVDHGTYPFVTSSSCAAGGACTGLGIPPSALGAVVGVMKAYTTRVG
                     SGPFPSELFDDVGERIRARGNEFGTVTGRPRRVGWFDAVVARTSVRVCGLDGIALTKM
                     DVLDDHEEIRIATGYTIGGKAVAEIPARADHYEQAQPVYRTFPGWKTSTVGIESWEAL
                     PARAREYVTALEEIVGAKVVILSTGPKREETIIRGGTVLDAWLGNVPVAQRG"
     gene            7720..9240
                     /gene="hutH"
                     /locus_tag="PLB01_11905"
     CDS             7720..9240
                     /gene="hutH"
                     /locus_tag="PLB01_11905"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HQQ78048.1"
                     /translation="MRPTILLDGDSLSLEELSLVSRGEAAAALAPRAAARVDAARTVI
                     DRAVAEDRVVYGVTTGFGKFADVVIPHAKLGELQLNLVRSHAAGTGPALASPAVRALM
                     LLRANCLAKGYSGIRRKTLELVLACLEAGVHPVVPEQGSVGASGDLAPLAHLALAFVG
                     EGEAEFRGRRMPSADALREANLAPAVLEAKEGLALINGTQLTTALGALALARLLHLLK
                     AIDLVGALTLDVLKGTDKAFDARIHAARPHPGQVASAANLRALLANSPLRESHRDCGV
                     VQDAYTLRCMPQVHGAARDAAAHARRVIEIEMNAATDNPMVFAEEGELVSGGNFHAAP
                     VALVCDLLAAAAADLASISERRVERLVNPSLSGDLPAFLVKDGGLNSGLMMIHVTAAA
                     LVSEAKANSFPASVDSIPTSAGKEDHVSMGPIAARKLARNVDLLETVLAIELLAAGQA
                     LDLRRPLRSSPPLEELHGRLRARVPFWDRDRAAAPAVEGARAVLTGDLGPILSPLA"
BASE COUNT         1381 a         3175 c         3312 g         1445 t
ORIGIN      
        1 gcacgccgag cgcgtccgcg acctcgccct cgcgctcttc gaccagacgc atgcgctcca
       61 ccagctcacc gcgctcgagc gcgaatggct cgagcacgcg gccctcctgc acgacctcgg
      121 gctctcggtc gcctacgccc gccaccacca ccactcgcac tacctgatcc gccacggcga
      181 cctcaagggc ttcacccagg acgaggtcga ggtgatcgcc ctcgtcgccc gctaccaccg
      241 caaggcgccg cccgacccct cgcacgaggc gttccggacg ctcgacccgt ggaagaagcc
      301 cgtggtcgag aagctctccg cgctgctccg ggtcgcggac gcgctcgacc ggacgcaccg
      361 agggctcgtg acgcgcgtcc gcgtcgtcct gcggggcaag aaggcccacc tgacgctcgg
      421 ggcgcgttcc gcgtgcgatc tcgagctctg ggcgctcaag aagaagggca agctcttcga
      481 gaaggttttc gggacgaaga tcgccgcaag ggtggttgtc ggataatcag cgggatgtcc
      541 ttcccccagg ccacggtgct cctgttcgcg gccgcggccg cggcgacggt cacccccacg
      601 cccacggccg cgccggcccc ggcggagacg ccgagaccgc gttcgctcgc ggacgtcgcg
      661 cgggaacgca agctcgtgcc gctgaagggt gcgggggagt ccccggtcgc cgggacgcag
      721 gggccgctgc gtgtcgagga gatccaggac aacggagccg tcgcggacgg gttcctctcg
      781 gtctacggcc gggtgcgcaa caccggccgc tcgccggtct gccgcgtgcg cctcttcctc
      841 cgcatgttcg acgagaacgg ggtcctcctc tcgaaggggg agacgacgac ggacctcaag
      901 gtcgttcccg cgggcgacgc cgtgccgttc ggggggcgtc tcaaggtgcc gcccggcgtg
      961 cgtggatcgc aggaacgggc tcccgacgtc ctcgccgagg gccccgcccg gacgaactcg
     1021 cagcgtgtcg cgcgcgtcga gggagaggtg ctggatttct cggaggagtg ccggtgagcg
     1081 ttctcgcgct cggattcgtc ctcctgcagg ccgccgcggc gacgccgtcg cccgctccga
     1141 cgcccacgcc ctcgccgcgt ccgtcggccg cggggccgcg cacgctccag gacgtcgcgc
     1201 gcgagcgcaa gctcgcgggc gcgcagaagg ggaagggctc gctcgtgacg atcagcggcg
     1261 ggccgtccac ggagacgccc gcacccgcgg gcactcccgc gcctgcggga actccggagg
     1321 ccggggccgc gacccccgag cccccggcgt cgacggcgag cgtgcgcgtg acggaggtcc
     1381 ggaacgacgg cgtcgtcgac ccgaacggcg gggtccgcgt gaacgggacg gtccgcaacg
     1441 gcgggtacaa ggcggcatgc aacgtcgtca tcacggtccg gatcatggac aacaagggca
     1501 tctacctcgc gagcggggag gccgctccgg acacggcggt gatcccgccg ggagcggtcg
     1561 tcagcttcca cacgatcgtc caggcgccgc ccggagtgcg gggcgcccgg gtggacccgg
     1621 accgccggga cgtcacggac ggatcgacga cgatggccgg ggactggaag ctgctcggcg
     1681 ggaccgaggc gaccgttgcg aacgcgaccg aggactgcgg ccgctgacgg cgggcttcag
     1741 cgcgccggcg gcttgccctt cgcgagcgct ttcttcgcct tgatgtagct gctcgagatc
     1801 gtcagcgtcg cgccgcgcgt gttcgggttg cggctgaaga gggcctcggt cgcttccggg
     1861 ccatgagcct tgttcttcca gaggaaatcc tcgatgaact tgttcggctg ctcgggccgg
     1921 ccccacccgt cgttcaggtc cttgaggatc cggtcggcgg acgccggggg aatctcgatg
     1981 tgcacgtcgg aaaggcggtt gctccagaag aggtagatga tcttgtcgac gggcgcgtcg
     2041 ctccacgtga ggtcgtcgtg ctcgaggcgg cagaacttct cctcagcgag ctcgtcgagg
     2101 acctccatct gcttggtcgg ctggtcgccc cacttcatct cgcgaaagcc gggcttcatc
     2161 ccgccgctcg cgcccccgcc cttgccgaag agagccatcc tggcattttc ctcccgcccg
     2221 atgaggatac tccggggccg gcaggaatcg gcgtcagggc ttcgggtccg cgagcccccg
     2281 cttgacgagg agcggctcga cgatcggctc ccgcccgcgg aagcgcttgt agagggtcat
     2341 cgcgtcctcg gtcccgccct tttcgaggat gttcttccgg aacgacgtcg ccgtcgcctt
     2401 gtcgaagatc cccttctcgc ggaacaggtc gtaggcgtcg gccacgagga cgtcgctcca
     2461 gatgtagctg tagtagccgg ccgagtagcc gccgccgggg ccgaagatgt gctggaagta
     2521 ggggctccgg taccggacga cgatctcggg gatcaggccg atcctgccga gcgaggcctt
     2581 ctcgaaggcg gaggcttcca tttccttcgg ctcgggaagg gtgtgccagt ccatgtccag
     2641 gtacgacgcc gcgaggtatt ccgtggtccg gaacccctcg ccgtacgtcc cggctttctc
     2701 gatcttcctc acgagatcga gagggatcga ctcgccggtc ttccagtgct tcgcgtagac
     2761 cttcaggacc tcgggctcga gcgcccagct ctccatgatc tgcgacggca gctcgacgaa
     2821 gtcgcgcgac gtggacccca tcgagcggta cgtgaccttc gagacgaagc cgttcaccgc
     2881 gtggccgaac tcgtggaaga gagtccggac ttcctcgagc gagaggaggg cgggggcgtc
     2941 gccggccggg cgcgtgaagt tcgtgacgtt cgtgacgatg ggccggacgt ccttgccctc
     3001 gcggacccat ccgcggcgga tccggccgca ccacgctccg ctccgcttgc cggggcgcgg
     3061 gtggtagtcg acggtgtaga gcgcgaggaa cgagccgtcg gcgtccttga cctcgaagac
     3121 gcggacctcc ggatggtact tcggcacgtc ggtccgctcc gtgaacgtga ggccgtagag
     3181 ccggttcgcg gcgtagaaca cgccttcgcg cacgcggtcg atcgggaagt acgggcggag
     3241 cgcctggtcg tcgaggtcga agcgcgcctt ccggaccttc tccgagtagt agcgccagtc
     3301 ccaggcctcg agcgcgaagt ccttcccgcc ggccttgacc gcggcctgga ggtcggcccg
     3361 ttctcgcttg gccatcgcga gggcgggcgt ccacacccgg tcgaggagcc cgtagacacc
     3421 ggccggcgtc ttcgccatgt tctcctcgag gacgaagtcg gcgtgggtct tgaagccgag
     3481 aaggcgcgct cgctcggaac gcagggcggc gatccgggag agcgtcgtct tgttgtcgag
     3541 ggcgtcgcca tggtcgccgc gcgacgtgta gccgtcgagg atcttcttcc ggagttcgcg
     3601 gttctccgcg aacgtcagga aggggaggat cgaaggggcc tgcggcgtga acgcccactt
     3661 gccctcgagc ttcgcggcct ttgcggcgtc ggccgccgcg gagacgagag ccgatggaag
     3721 gcccgcgagg tcttccttgc ggtcgacgac gagcctgaag ccgttcgtct ccttcaggag
     3781 gttgtcggcg aacttcaggc cgaggacgga cagctcctgg ttgatctcgc ggaagcgctt
     3841 cttctgctcg ggcgcgaggt tggcgccgcc gcggacgaag tccttccagg tctcctcgag
     3901 aagcttcttc tgctcgacgg tgagcggcag cttctcgcgc gcctcccaga ccgccttcac
     3961 ccgcgcgaag agcttttcgt tcagaaggac gtcgtcgcgc agggccgaga gcttcggcgc
     4021 gacttccttg ttgatcgcct ggagcgcgtc gttcgtgtcg gcgcccgtca gcgcgaagaa
     4081 gacgtcgctc accttcgcga gggcgagtcc gccgttctcg agcgcctcca ccgtgttcgc
     4141 gaacgtcggg gccgcggggt tcgcggcgat cgcctcgact tccttcttcc gctcggcgat
     4201 cccggcttcg aaggccggct tgaagtgctc ggccttgatc cgctcgaacg gcggcatccc
     4261 gaacggcgtc gtccactcct cgaagaacgg gttgggcggg ggcggggcct gcgcggcggc
     4321 gggaacggcg gcgaagagga cgatcacggc gacggcgaga cgggaaaacg cgagcggcat
     4381 gggtgccagc cctccttcat gcggacgatc agccgacgtt agcgccttcg cgccggctga
     4441 gcgcctgatg cagatgaagg atgggccgtc agggcagcgc gccgggtggc ggcgcgacga
     4501 tgcacgctct ccaacggttg gaccattcgc gctgaaccca gtagccgtcg agcatttcgt
     4561 ccctgtctgc cacggaagcg caaatgtccg cgatctcggc ctggtcaggc tgagggtcga
     4621 accaggccgc aggccgagtc agtgtcgctc cgatctcgcc gatcgcaggg tcggttaccg
     4681 cttcgacgag ctcgtgagat gcgttcgacg tcgcgatgtc gaacgggttg gggtgtgcgc
     4741 cgcagccggt caggcagccg ccggagatgg ccgggaggac accgtatgcg gaccggacgc
     4801 cattgatccg cgtcgaggag tgatacgcgc agaaattcac acacgacctg aggccggccc
     4861 cgtcgtcgat ctgcacgctg tccgggaagt cgatcatgta gaccgtcacc gggtttccct
     4921 gggggtccac ctcggggggc gggatggctc cagatgcgac atgcgccgcg agcgcggcgg
     4981 tgatctgcga gtcggtgacc gtcgccccct gagaaggcgt catcgtgtac gtgcccgcga
     5041 aggagccgtg gccgatgcgc tggtccgagc cggacaagcc gtccgcaaag cccgtcaggc
     5101 cgacggtgtc gtattcggaa agccagtcga acatcgcgcc cttcgtgagg gagcggtaga
     5161 acccctccat ggcggctgaa tggtccgccg gcaccgtcgc attccaccgg accgcgacca
     5221 ccttcgcgtt cgatacgacc ggtccgccgt agtactcgag gtgcggcttt acccgacggc
     5281 ggaccctcct aagcgacgac gagacgggcg gctgcgcgac gagcgcgagg cggtcggcgg
     5341 cggagtcctc gttgccacag ggcaccgcga cgtccacgaa gagcggcttg aagttgcgag
     5401 ggagggaagc cgccgggcga cgcacgacac acgtccgctc atccggggac gccgcgaacg
     5461 ccccgccggc cgccgtgagg gcgagggcaa acacgagacc acgtctcaag tgtgggaatg
     5521 gtacccccag agggattcga accctcgatc tccaccttga aagggtggcg tcctgggccg
     5581 ctagacgatg ggggcacccg aggaacggcg gattctagcg gcttttcgct ccggcgtcag
     5641 tccggctgga cgaaagcctg ctcgaaccgg cggacgatgt cgtcgacgct cggctccgcc
     5701 ttgacgcccg tgcgcgaaaa gacccccgaa attttcttca ggaccgaggg cgcgcccgcg
     5761 ggccgcgcag cctgcgcctc cttgaggccc tggtgcacct cgcgcacgac gcgcgggtgc
     5821 ggctcgtggt cgctccgcac cgagtgcttg aggagttcgg cattggagat cctcgggctc
     5881 ttggccgagc gcaggacctc gaaggcctcg gccgaggccc tgagacggag actccgtttc
     5941 gcaggggaaa tgaccatggt cggtcctccg gacgggttct gggttccctt tgaccgctga
     6001 ggcctggatc ttacgacaca ggctcggggt ttcggtcaag aacgaaacaa ggtggtgagc
     6061 cgtgtcggac tcgaaccgac gaccccctgc ttaaaaggca ggtgctctaa ccgactgagc
     6121 taacggccct caccaacggc agtctatccg acgacggcgg agtccccgaa gcgccctcag
     6181 ccccgttgcg ccaccgggac gtttccgagc caggcgtcca ggaccgtgcc gccgcggatg
     6241 atcgtctcct cgcgtttcgg gccggtcgag aggatcacga ccttcgcgcc gacgatctcc
     6301 tcgagagccg tgacgtactc gcgggcgcgg gcggggaggg cctcccagct ctcgatcccg
     6361 acggtcgacg tcttccatcc ggggaacgtc cggtagacgg gctgggcctg ttcgtagtgg
     6421 tcggcgcgcg ccgggatctc ggcgacggcc tttccgccga tcgtgtagcc cgtcgcgatg
     6481 cggatctctt cgtgatcgtc gaggacgtcc atcttcgtca gggcgatgcc gtcgaggccg
     6541 cacacgcgga ccgaggtccg cgcgacgacc gcgtcgaacc agcccacgcg ccgcgggcgg
     6601 cccgtcacgg tgccgaactc gttgccgcgc gcgcggatcc gttcgccgac gtcgtcgaag
     6661 agctcggacg ggaatgggcc cgagccgacg cgcgtcgtgt acgccttcat gacgccgaca
     6721 acggcgccga gcgccgacgg cgggatcccc aggcccgtgc aggcgccgcc tgccgcgcac
     6781 gaggaggacg tcacgaacgg gtaggttccg tggtccacgt ccagcatgac gccctgcgcg
     6841 ccctcgcaga ggagcttctt gccggccgcg agctgcgcgt tcagccaggc gctcgtgtcc
     6901 gtgacgtggc gcgcgagttt tttaccgcat tccacgtaag tgtcgagcgt ctcctcgatc
     6961 ggagcgggct cgacgccgta gaagtgcgag agaaccgcgt catggtgggt gaggacgggg
     7021 cgcgcgagcg cgcggaagcg gtcaggatcc acgaggtccg ccatgcgcag gccggtgcgg
     7081 ttcgccttcg tctcgtacgc ggggccgatg ccgcgcgacg tcgtcccgat gttcgcgccg
     7141 ccgagcgcct tctcgcgcgc gccgtcgagg agggcgtgcg tcggcaggat gacgtgcgcg
     7201 cggtccgaga tgagcaggtt ctcctcgatg cggacgccgg cggcgcgcag gctgtccatc
     7261 tcggagagca tcgcccgcgg gtcgatcacg aggccgttgc cgacgacgtt catgacgccg
     7321 gggcggcaga tgccggacgg gatgaggcgg agcgcgaagt ggcggtcgcc gaacttcacg
     7381 gtgtggccgg cgttgtggcc gccctggtag cgggcgacga ggtcgaacga gggggagatg
     7441 aggtcgacga ccttgccctt gccctcgtct ccccactggc cgccgacgat ggcgagggcg
     7501 gagcccttga tgtgtgtcac ttgcgaattc taccggagga agaggagagg ttgagtaaga
     7561 gaagagaaag aaagagaaag aaagagaaag agaagatttc ctagaagggt gagtggggcc
     7621 ggggcgacgg agccggcgcc gcggtgactc gaggccggag cccgtattca ccttctaaga
     7681 agatccccgg atccctttcc cggtgcacaa tgtctcatcg tgcgacccac cattcttctc
     7741 gacggggatt ctctttcact cgaagaactc tctctcgtct cccgcggcga agccgcggcg
     7801 gcgctcgcgc cgcgcgccgc ggcgcgcgtg gacgcggcac ggaccgtcat cgacagggcc
     7861 gtggcggagg accgggtcgt ctacggcgtc acgacgggct tcggaaagtt cgccgacgtc
     7921 gtcatcccgc acgcgaagct cggcgagctg cagctcaacc tcgtgcggag ccacgcggcc
     7981 ggcaccgggc cggcgctcgc ctcgcccgcc gtccgcgcgc tcatgctcct gcgcgcgaac
     8041 tgcctcgcga agggctactc cggcatccgg aggaagacgc tcgagctcgt cctcgcgtgc
     8101 ctcgaggcgg gggtgcaccc ggtcgtcccc gaacaggggt ccgtcggcgc gtcgggcgac
     8161 ctcgcgccgc tcgcgcacct cgcgctcgcc ttcgtcgggg aaggcgaggc cgagttccgc
     8221 gggcggagga tgccctccgc ggacgcgctg cgcgaggcga acctcgcgcc ggcggtcctc
     8281 gaggcgaagg aagggctcgc gctcatcaac gggacgcagc tcacgacggc gctcggcgcg
     8341 ctggcgctcg cgcgcctcct ccacctcctc aaggcgatcg acctcgtcgg ggcgctcacg
     8401 ctggacgtcc tgaaggggac cgacaaggcc ttcgacgcgc gcatccacgc ggcgcgcccg
     8461 catccgggcc aggtcgcgtc ggccgcgaac ctccgggcgc tcctcgcgaa ctcgccgctc
     8521 cgggagagcc accgcgactg cggggtcgtg caggacgcct acacgctgcg ctgcatgccg
     8581 caggtgcacg gcgcggcgcg cgacgcggcc gcccacgccc ggcgcgtgat cgagatcgag
     8641 atgaacgccg cgacggacaa cccgatggtc ttcgccgagg agggcgagct cgtctcgggc
     8701 ggaaatttcc acgcggcgcc ggtcgccctc gtctgcgatc tcctcgcggc cgcggcggcg
     8761 gacctcgcgt cgatctccga gcgccgcgtg gagcgcctcg tgaacccgtc gctctcgggc
     8821 gacctcccgg cgttcctcgt gaaggacggc ggcctgaatt cgggcctcat gatgatccac
     8881 gtgaccgcgg cggcgctcgt ctcggaggcc aaggcgaatt cgttcccggc ctcggtggac
     8941 tcgattccga cgtcggccgg caaggaagac cacgtgtcga tgggcccgat cgccgcgcgg
     9001 aagctcgcgc gcaacgtcga ccttctcgag accgtcctcg cgatcgagct cctcgccgcg
     9061 gggcaggcgc tcgacctccg gcgcccgctc cgctcgtcgc cgccgctcga agagctgcac
     9121 gggcggctgc gcgcgcgcgt ccccttctgg gacagggacc gcgcggccgc tccggccgtc
     9181 gaaggcgcgc gcgccgtgct gacgggcgac ctcgggccga tcctgtcccc gctcgcctga
     9241 atcagggcgc gagcgcgtcc gcgagggcgg acgggagctc gtcggagggg accgcgcttt
     9301 cgaggagccc cag
//
LOCUS       DAOMFY010000142         9270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2267_length_9270_cov_6.916658, whole genome shotgun sequence.
ACCESSION   DAOMFY010000142 DAOMFY010000000
VERSION     DAOMFY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2267_length_9270_cov_6.916658"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1254
                     /locus_tag="PLB01_11910"
     CDS             82..1254
                     /locus_tag="PLB01_11910"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HQQ78049.1"
                     /translation="MSRAAAVALLACLSTGTLCGEEPRPVSSVAGRTDLRAAVALGCA
                     ATTVLLGEASHGTASPIELKAALVKELITRCGFTHVVFESQLYDFEALKRKYDSSSAS
                     MADLLNAIGGLWATSGELDDLARFLHERARAHELTLSGIDGQVGGAMNLYAQTALAAD
                     LTTRLPPERRTHCTAEIQRLNQWRFDASHPYDERFRAGLSDCLRDAELATPEADVGRR
                     LLLSSYAGFLKLPAQDFANQRDRLMFQNFSSIMRSMPRRTKSIIWTANRHALLAEYRG
                     IVSLGSLLSQESPGAVKSFACVAKEGSYGFQGKPAQAIGAAPQGTLKADALSGSGETG
                     VTFLAGPELARLSGRPSRLLGYGEFLEGPWAKLFDGVFIARSERMPAYVRPRAPQQ"
     gene            complement(1304..3157)
                     /locus_tag="PLB01_11915"
     CDS             complement(1304..3157)
                     /locus_tag="PLB01_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78050.1"
                     /translation="MADHHEEEVLGKAVEPRLLRRLFGLAKPYRAYAAGSLAVLLLES
                     LAQLAGPLLTAAAIDLVLTRERTGAGPAAKGVDRFFAAFGIDLAAGHGIEWIAALSVA
                     TALLAFVLMMVMVWWTSTMGQGVMYDLRTRIFGHLQRADVAVFDRTPVGRLMTRLTTD
                     VDALNELFTSGFVALLGDVVVLAGIVGILFALDPSLAAITFGILLPMLWVTNWFRKGA
                     RDTYRDVRVKIARVNAFLQEHISGIAIVQLFRRERVAAKAFSEVNDAHRAANIESIFY
                     YSVFYPALDFLSALGMASIVWYGGWQVLRGRMTIGAVVAFLQYSKRFYRPLMDLSEKY
                     NILQAAMASSERIFQLLDAEPAITAPASPVVPAGPLRGEIAFEDVRFSYKAGEEILKG
                     VSFHVPPGQTVALVGATGAGKSTILNVLLRFYDVTSGRVLVDGEDVRRYHPVRLREHF
                     GIVLQDTFLFTGTIAENVSFFDPDMPRARIEEACREVGLGPLLARIPGGLDAKLTERG
                     GGLSVGEKQLVSFARALARDPRILILDEATSSVDPETEKTIERAVQRVLAGRTSLVVA
                     HRLSTIVRADRILVLSHGEVKESGTHAELLEKGGLYARLYALQLKDREAAA"
     gene            complement(3157..4884)
                     /locus_tag="PLB01_11920"
     CDS             complement(3157..4884)
                     /locus_tag="PLB01_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78051.1"
                     /translation="MRGLPRFLGYFRRRPGVYAAGFGTMLASTGLFLAMPGLVRRAIE
                     ELGTGVTTERLFRLSATIVLLAVGDAIALFLTRRLLIGASRDIEYEMRQDLFAHLLRL
                     PPVWYRRNRVGDLMSRAVNDLSAVRMLLGPGIMQAGNTLLVGTVSLVLMFRVSPALTG
                     VALAVFPVIAIATKVMGQATHKRFTKIQEFFSEISAEAQENFTGQRLVRAFAREEREA
                     ARFDANNLEFRRRNLGLARLNALFFPLLQFLVGLGFALTLWAGGRFILAGKLSVARYV
                     EFNLYMIELIWPAIALGWVVNLWQRGAASWNRMMDVWDAAPLPEEAEGGAPLSGDVEF
                     RNLTWGYGDRPVLEGVSFRVPAGSTVAIVGRTGSGKSTLLNLLLRVDDPPAGSVLVGG
                     RDLSTEPLPLVRRSLAVVPQETFLFSDTLAANVAFARPDASRAEVEAAVFGAGLAPDV
                     ARFPKGLDTVVGERGITLSGGQKQRTALARALLSDAPILVLDDAFSSVDTETEARTLQ
                     GLRGAAGGRTVFLVSHRLSTLRNADFLVVLDHGRVAETGTHEELLARGGVYAAIAERQ
                     RLAEEMEAA"
     gene            4929..7370
                     /locus_tag="PLB01_11925"
     CDS             4929..7370
                     /locus_tag="PLB01_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQQ78052.1"
                     /translation="MDSQTTRHTDPVCGMTVDPARAAATVNWGDKDWYFCAKSCAEKF
                     RRDPERFSANEKSAATFEPMTAPVGLGLPSLTLPSAQATPMAMPAAQPHAPSKKSPSS
                     LSSSKWICPMDPVVVSDVPGACPICGMALEPAVATGEEGNPELDDMQRRFRVALALTL
                     PLFLFEMSAMAGLRLFERLPPGLNLLVQLALATPVVLWCGKPFFERAWLSLKTRNFNM
                     FTLIGLGTGAAYLYSLARTAQSLLPIQRNLSSSLPLHSAAHDVYFEAAAGIVTLVLLG
                     QVLELRARAKTSGAIRELLSLAPDSARVVRKGAEVDIPLDEVMKGDVLRVRPGERVPV
                     DGTVTEGASAVDESMLSGEPMPVEKAPGAKVTGGTLNGTGSFLMRAERVGADTVLARI
                     VALVGEAQRSRAPVQRLADRVAGIFVPAVVAAAAVTAVAWGFFGPEPRLPHALVNAVA
                     VLIIACPCALGLATPMSVMVGVGRGARSGVLFRDAAALETLGQCDTLVLDKTGTITEG
                     KPKIVETRAFGAFDESELLRVAASLERASEHPLAAAVIRAAEARGLALTAATEFKASP
                     GKGIQGRVDGRSVELGSLSLFEETLQRRYPREQLEAAGSWRRRGATILFMAVDGEPAG
                     FLAAADPIKNSAKAAIAELSALGLRVVMLTGDAEATAKAVASEAGIEEVVAGVLPAGK
                     ADEVSRLKKAGLRVAMAGDGVNDAPALAAADVGIAMGTGSDVAIESASVTLAGGDVAA
                     LPKAVRLSRATLRNIRQNLFFAFVYNVVGVPVAAGVLYPAFGLLLSPVLASAAMTFSS
                     LSVVGNALRLRSVRL"
     gene            7444..7992
                     /locus_tag="PLB01_11930"
     CDS             7444..7992
                     /locus_tag="PLB01_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78053.1"
                     /translation="MTQAVLVVALFALVAGGVVLLRSWWAWRQRRLITCPENEQPAAV
                     RVAALGAAAGSLAGHPHLKLSECSRWPEKKDCGQECLVQIEKGPSECLVRTLVARWYE
                     DKPCACCGKAIRDVAWADQRPGLRAPDGRYIAWPEVPPEALPETFKTHAPVCWNCLVV
                     QKIAREHPDDLTVRPPREQLYS"
     gene            8049..8852
                     /locus_tag="PLB01_11935"
     CDS             8049..8852
                     /locus_tag="PLB01_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78054.1"
                     /translation="MEASPRPAFELSKWYLDAVGEDGEVFIGYRAALRWRRLRASYSA
                     ALTGGADESRTASSVTSGAEPVLQMGSLVWAAPAIGIEGTWTEDAPALLRTLYDGPGG
                     SVVWACLFPRARAGVRIGSRELRGFGYAERLEMTLPPWKLPLDTLRWGRFLSPDRWVV
                     WIDWQAPARSRTWVFVDGIEAREAEVSDEEISFEGGRVRLPSASRLALRAGRVSDLLR
                     GLPLSLLLRLFRRARAMHETKWRTRGVLEFAGAPAVEGWAIHEVVRFRA"
     gene            8849..>9270
                     /locus_tag="PLB01_11940"
     CDS             8849..>9270
                     /locus_tag="PLB01_11940"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQQ78055.1"
                     /translation="MNERAGKFLYGSFFAVVLPALLVLWARAAGPNVGLPAVESPVGW
                     GVAAFGLGLLLWATAALAVFGRGLPMNAYPPPVFVGRGPYAVVAHPIYLGFVLVCAGC
                     AIGSGSAAGLWLVTPAVALGSAALVLGYEGPELDVRFGP"
BASE COUNT         1373 a         3158 c         3352 g         1387 t
ORIGIN      
        1 cgcgctgggc gggcgagccg cccccgcgct gacccgcgga cgaggtgtac gagaatgccc
       61 gggatagggg gacgccggag catgtccagg gccgccgcgg tcgcgttgct cgcgtgtctc
      121 tccacgggca ccctctgcgg tgaggagccc cggcccgtat cctcggtcgc aggccgcacc
      181 gatcttcgag cggcggtcgc tctcggttgc gccgccacca ccgttctcct gggagaagcc
      241 tcgcacggaa ccgcgagtcc gatcgagctc aaggccgcgc tcgtcaagga gctgatcacc
      301 cgttgcgggt tcacgcacgt cgtgttcgag agccagctct acgacttcga ggccctcaaa
      361 cggaagtacg acagctcctc ggcctcgatg gcggaccttc tcaatgcgat cggaggactc
      421 tgggcgacct ccggggagct ggacgacctc gcccgctttc ttcacgagag agcccgggca
      481 cacgagctca cgctgagcgg catcgacggg caggtcggag gggcgatgaa cctctacgcg
      541 cagaccgcgc tggcggccga cctgacgacc cgccttcccc cggaacggcg cacgcactgc
      601 accgccgaaa tccagcggct caaccagtgg cgtttcgacg cgagccaccc ctacgacgag
      661 cggttcaggg cgggcctgtc ggactgcctc cgagatgcgg agctcgcgac tccggaggcc
      721 gacgtcggcc ggcggctcct cctctcgagc tatgcgggct ttctgaaact cccggcccag
      781 gacttcgcga accagcggga ccggctcatg ttccagaatt tctcctcgat catgcggtcc
      841 atgccccggc ggacgaagag catcatctgg acggcgaatc gccacgccct tctcgccgag
      901 taccgcggca tcgtctcgct ggggagtctc ctgtcccagg agagccccgg agccgtcaag
      961 tccttcgcgt gtgtcgcgaa ggaagggtcg tatgggttcc agggaaagcc cgcccaggcg
     1021 atcggcgcgg ctccgcaagg cacgctcaag gccgatgctc tctccggcag cggcgaaacc
     1081 ggagtcacct ttctcgcggg tccggagctg gcccgcctga gcgggcgccc gtcccgcctc
     1141 ctcggatacg gcgaattcct ggaaggcccc tgggcgaaac tgttcgacgg cgtgttcatc
     1201 gcccggagtg aacggatgcc cgcatacgtc cgcccacggg cgccgcagca atagcggcgg
     1261 gcgcgaagac gtcagaggtc ttcgtcccgg ccgggagtcc cgctcaggcg gccgcttcgc
     1321 ggtccttgag ctggagcgcg tacaggcggg cgtagagacc gcccttctcg aggagctcgg
     1381 cgtgcgtgcc ggattccttc acctcgccgt gcgagaggac gaggatccgg tcggcacgca
     1441 cgatcgtcga caggcggtgg gcgacgacga gcgacgtgcg gcccgcgagg acgcgctgga
     1501 ccgcgcgctc gatcgtcttc tcggtctccg ggtccaccga ggacgtcgcc tcgtcgagga
     1561 tgaggattcg cgggtcgcgg gcgagggcgc gcgcgaacga gacgagctgc ttctcgccca
     1621 ccgagaggcc gccgcccctc tccgtgagct tcgcgtcgag ccctcccggg atgcgcgcga
     1681 ggagcgggcc gaggcccacc tcgcggcagg cctcctcgat gcgcgcgcgc ggcatgtcgg
     1741 ggtcgaagaa cgagacgttc tccgcgatcg tgcccgtgaa gaggaacgtg tcctgcagga
     1801 cgatcccgaa gtgctcgcgc agccgcacgg ggtggtagcg gcgcacgtcc tcgccgtcga
     1861 cgaggacgcg gcccgaggtc acgtcgtaga aacgcagcag gacgttcagg atcgtggact
     1921 tgccggcgcc cgtcgcgccg acgagcgcga ccgtctggcc cggcggcacg tggaacgaga
     1981 cgcctttgag gatctcctcg cccgccttgt acgagaacct cacgtcctcg aaggcgatct
     2041 ccccgcgcag cggcccggcc gggacgacag ggctcgccgg cgcggtgatc gcgggctcgg
     2101 cgtcgaggag ctggaagatc cgctcggacg aggccatcgc ggcctggagg atgttgtact
     2161 tctccgagag gtccatgagc ggccggtaga agcgcttgct gtactggagg aacgcgacga
     2221 cggcgccgat cgtcatccgc ccccgcagga cctgccagcc gccgtaccag acgatcgagg
     2281 ccatgccgag cgccgagagg aagtcgagcg ccgggtagaa gaccgagtag tagaagatcg
     2341 actcgatgtt cgcggcgcgg tgcgcgtcgt tgacctcgga gaaagccttt gcggcaacgc
     2401 gctcgcggcg gaagagctgg acgatcgcga tcccggagat gtgctcctgg aggaacgcgt
     2461 tcacccgcgc gatcttcacg cggacgtcgc ggtaggtgtc gcgcgcgccc ttgcggaacc
     2521 agttcgtgac ccagagcatc gggagcagga tcccgaacgt gatcgccgcg agcgaggggt
     2581 cgagcgcgaa gaggatgccg acgatcccgg cgagcacgac gacgtcgccg aggagcgcga
     2641 cgaagccgga cgtgaagagc tcgttcaggg cgtcgacgtc cgtcgtgagt cgcgtcatga
     2701 ggcgcccgac gggcgtccgg tcgaagacgg cgacgtccgc gcgctggagg tggccgaaga
     2761 tccgcgtcct caggtcgtac atgacgccct gccccatcgt ggacgtccac cagaccatga
     2821 ccatcatgag gacgaacgcg aggagcgccg tcgcgaccga gagcgctgcg atccactcga
     2881 tgccgtggcc ggccgcgagg tcgatcccga aggcggcgaa gaaacggtcg acgcccttcg
     2941 ccgcggggcc ggcgcccgtc cgctcgcgcg tgaggacgag gtcgatcgcg gcggcggtca
     3001 ggagggggcc ggcgagctgc gcgagagact cgaggagaag caccgcgagc gagccggccg
     3061 cgtaggcgcg atacggtttc gcgaggccga agagacggcg caggaggcgc ggctcgacgg
     3121 ccttcccgag gacctcttcc tcgtggtggt cggccatcag gcggcctcca tctcttcggc
     3181 gaggcgctgc cgttccgcga tcgcggcgta gacgccgccc ctggcgagca gctcctcgtg
     3241 cgtgcccgtc tcggcgacgc gcccgtggtc gaggacgacg aggaaatccg cgttgcggag
     3301 cgtggagaga cggtgcgaga cgaggaagac ggtccgtcct cccgcggcgc cccggaggcc
     3361 ctggagggtg cgcgcctccg tctcggtatc gacggaggag aacgcgtcgt cgaggacgag
     3421 gatcggggcg tccgagagaa gcgcgcgcgc gagcgcggtg cgctgtttct gtccgccgga
     3481 gagcgtgatc ccgcgctcgc ccacgacggt gtcgagccct ttcgggaagc gcgcgacgtc
     3541 gggggcgagg ccggctccga agaccgcggc ctcgacctcg gcacgggacg catccggccg
     3601 cgcgaaggcg acgttcgcgg cgagcgtgtc cgagaagagg aacgtctcct gcggaacgac
     3661 cgcgaggctc cgccgcacga ggggaagcgg ctccgtcgag aggtcgcggc ccccgacgag
     3721 gaccgatccc gcaggcgggt cgtccacgcg caggagcagg ttcaggagcg tggatttccc
     3781 cgagccggtg cggccgacga tcgcgaccgt gctccccgca gggacgcgga aggagacgcc
     3841 ctcgaggacg ggccggtcgc cgtagcccca cgtgaggttc cggaactcca cgtcgcccga
     3901 gagcggcgct ccgccctcgg cctcttcggg caggggagcc gcatcccaga cgtccatcat
     3961 ccggttccac gaggccgcgc cgcgctgcca gaggttcacg acccagccga gggcgatcgc
     4021 gggccagatg agttcaatca tgtagaggtt gaactcgacg tagcgcgcga ccgacagttt
     4081 gcccgcgagg atgaagcgtc cgcccgccca cagcgtgaga gcaaagccga ggccgacgag
     4141 gaactgcagg agggggaaga agagggcgtt gagccgcgcg aggccgaggt tgcggcggcg
     4201 gaactcgagg ttgttcgcgt cgaaccgggc cgcctcgcgc tcctcccggg cgaacgcgcg
     4261 cacgagccgc tggcccgtga agttctcctg cgcctcggcc gagatctcgc tgaagaactc
     4321 ctggatcttc gtgaagcgct tgtgcgtcgc ctggcccatc accttcgtcg cgatcgcgat
     4381 gacggggaag acggcgaggg cgacgcccgt cagggcgggc gacacgcgga acatgaggac
     4441 gagggacacc gttcccacga ggagcgtgtt ccccgcctgc atgatcccgg gcccgagcag
     4501 catccggacg gcggagaggt cgttgacggc gcggctcatg aggtcgccga cgcggttccg
     4561 gcggtaccac acgggcggca ggcgcaggag gtgagcgaag aggtcctgcc gcatctcgta
     4621 ctcgatgtcg cgcgaggcgc cgatcaggag ccggcgcgtc aggaagagcg cgatcgcgtc
     4681 gccgacggcg aggaggacga tggtcgcgga aagccggaag agccgctccg tcgtgacccc
     4741 cgtgccgagc tcctcgatgg cacgccgcac gaggcccggc atcgcgagga agagcccggt
     4801 cgacgcgagc atcgtcccga agccggcggc gtagacgccg ggccggcggc ggaaatagcc
     4861 gaggaaacgg ggaagccctc tcactccggg aatatagccg acgccgccga tggttaaatg
     4921 ctgacccgat ggattcacag acgacccggc acaccgaccc cgtctgcggc atgacggtcg
     4981 atccggcccg cgcggccgcc accgttaact ggggcgacaa ggactggtac ttctgcgcga
     5041 agagctgcgc ggagaaattc cgaagagacc cggagagatt ttctgcgaat gagaagagcg
     5101 cggcgacatt cgagccgatg acggcgccgg tgggcctggg gctcccatcg ctgaccctgc
     5161 cgtcggccca ggccacgcct atggcgatgc cggccgccca gccccatgca ccttctaaga
     5221 aatctccttc ttctctttct tcgtccaagt ggatctgccc gatggacccc gtggtcgtgt
     5281 cggacgtgcc gggcgcgtgc ccgatctgcg gcatggcgct cgagccggcc gtcgcgacgg
     5341 gcgaggaagg gaaccccgag ctggacgaca tgcagcggcg ttttcgtgtc gcgctcgcgc
     5401 tgacccttcc gctgttcctc ttcgaaatga gtgcgatggc gggacttcgg ctctttgagc
     5461 gcctcccgcc gggactcaac ctgctcgttc agctcgctct cgcgacgccc gtcgtcctct
     5521 ggtgcgggaa gccgttcttc gaaagagcct ggctgtcgct gaagacgcgg aacttcaaca
     5581 tgttcacgct catcggcctc ggcaccggcg ccgcatatct gtacagcctg gcgaggaccg
     5641 cgcagtccct cttgcccatt caaagaaatc tctcttcttc tctccctctt cattccgcgg
     5701 cccacgacgt ctacttcgag gcggcggcgg gcatcgtgac gctcgtcctc ctcgggcagg
     5761 tgctcgagct gcgcgcgcgg gcgaagacgt ccggcgcgat ccgggagctg ctctcgcttg
     5821 cgcccgactc ggcgcgtgtc gtgcggaagg gcgccgaggt cgacatcccg ctcgacgagg
     5881 tgatgaaggg agacgtcctg cgcgtgcgcc ccggcgagcg cgtgcccgtg gacgggacgg
     5941 tgacggaggg cgcgagcgcc gtcgacgaat cgatgctgag cggcgagccg atgcccgtcg
     6001 agaaggcccc cggcgcgaag gtcacgggcg ggacgctcaa cggcacgggc agcttcctca
     6061 tgcgcgcgga gcgcgtcgga gccgacaccg tgctcgcgcg gatcgtggcg ctcgtgggag
     6121 aggcccagag gagccgggcg cccgtccagc gcctcgcgga ccgggtcgcc gggatcttcg
     6181 tgcccgcggt cgtcgcggcc gcggcggtca cggcggtcgc ctggggcttc ttcggccccg
     6241 agccgcgcct cccgcacgct ctcgtgaacg ccgtcgcggt cctcatcatc gcgtgcccgt
     6301 gcgctctcgg cctcgcgacg ccgatgtccg tcatggtcgg ggtcgggcgc ggcgcgaggt
     6361 cgggcgtcct cttccgcgac gcggcggcgc tcgagacact cggccagtgc gacacgctcg
     6421 tcctcgacaa gaccgggacg atcacggagg ggaagccgaa gatcgtggag acccgcgcct
     6481 tcggcgcctt cgatgaaagt gaattgctgc gggtggccgc gtcgctggag cgcgcgagcg
     6541 aacaccccct cgcggcggcc gtcatcaggg cagccgaggc gcgggggctc gcgctcaccg
     6601 cagcgaccga attcaaggcc tcgcccggaa agggcatcca gggcagagtg gatggacggt
     6661 ccgtcgaact cgggtctctc tcactcttcg aagaaactct tcagcgacgc tatccgcgcg
     6721 aacagctgga ggcagcagga agctggaggc gcaggggcgc cacgatcctg ttcatggcgg
     6781 tcgacgggga gcccgccggc ttcctggccg ccgccgaccc gatcaagaac tccgcgaagg
     6841 ccgcgatcgc cgagctttcg gcgctcggcc tccgcgtcgt gatgctcacc ggcgacgccg
     6901 aggcgacggc gaaggccgtt gcctcggagg cggggatcga ggaggtcgtt gccggcgtgc
     6961 tccctgcggg caaggcggac gaggtctcgc gcctgaaaaa ggcgggcctc cgggtcgcga
     7021 tggcgggcga cggcgtgaac gacgcgccgg cgctcgcggc cgcggacgtg ggcatcgcga
     7081 tggggacggg ctcggacgtt gcgatcgaga gcgcctcggt caccctcgcg ggcggcgacg
     7141 tcgcggcgct cccgaaggcg gtgcgcctct cgcgtgcgac gctccgcaac atccggcaga
     7201 acctgttctt cgccttcgtc tacaacgtcg tcggagtgcc cgtggccgcg ggcgtgctct
     7261 acccggcgtt cggtctcctg ctgtcgccgg tcctggcgag cgccgcgatg acgttcagct
     7321 cgctctcggt cgtgggaaac gcgctgcgcc ttcggagcgt ccgcctctag acggctgatt
     7381 ccggcgcatc atggccgccg gcccccggcc gagccgaagc tgacccgggg gtgttccatg
     7441 tccatgaccc aggccgttct ggtggtcgcc ctcttcgccc tcgtcgcggg cggtgtcgtc
     7501 ctccttcggt cctggtgggc ctggaggcag cggcgcctca tcacgtgccc ggagaacgag
     7561 caacccgcgg ccgtgcgcgt cgccgcgctc ggcgccgcgg ccggctccct ggccggacac
     7621 ccgcatctca agctctccga gtgctcgcgc tggcccgaga agaaggattg cgggcaggag
     7681 tgcctcgtcc agatcgagaa aggcccgagc gagtgcctcg tgcggaccct cgtggcgcgc
     7741 tggtacgagg acaagccgtg cgcgtgctgc gggaaggcca tccgtgacgt ggcctgggca
     7801 gaccagaggc ccggcctgcg cgcgcccgac ggccggtaca ttgcgtggcc ggaggttccg
     7861 ccggaggcgc tcccggagac gttcaagacg cacgcgccgg tctgctggaa ctgcctcgtg
     7921 gtgcagaaga tcgcccggga acaccccgac gatctgaccg tccgcccccc gcgggaacag
     7981 ctgtatagct agtcgttccc tgaaaattcc cgaggacttc cagccggacg aggccccacg
     8041 tttgaggcat ggaagcgtca ccgcgtcccg ccttcgagct ttcgaagtgg tacctcgacg
     8101 cggtcgggga ggacggagag gtcttcatcg ggtatcgcgc ggcgctccgg tggcggcgcc
     8161 tgcgggcctc gtactcggcc gcgctgacgg gcggggccga cgagtcgcgg acagcctcga
     8221 gcgtgacgtc cggggcggag ccggtcctgc aaatgggctc cctcgtgtgg gcggcgccgg
     8281 ccatcgggat cgaggggacg tggacggagg acgcgcccgc gctccttcgg acgctctacg
     8341 acggccccgg cggatcggtc gtctgggcct gcctcttccc gcgcgcgcgg gccggcgtgc
     8401 ggatcggttc gcgcgagctc cgcggcttcg gctacgccga gcgactcgag atgacgctcc
     8461 cgccctggaa gctgccgctc gacacgctgc gctggggccg gttcctttcc ccggaccggt
     8521 gggtcgtctg gatcgactgg caggcgccgg cgcgctcacg cacatgggtc ttcgtggacg
     8581 ggatcgaagc gcgggaagcg gaggtttcgg acgaagagat ttcttttgaa ggtggaagag
     8641 ttcggctccc ttcggcaagc cggctggcgc tgcgcgccgg ccgcgtctcc gaccttctga
     8701 ggggtcttcc cctttctctc cttcttcgtc tctttcgtcg cgcgcgcgcc atgcacgaga
     8761 cgaagtggcg gacgcggggc gttctcgaat tcgccggcgc gccggccgtc gagggctggg
     8821 cgatccacga agtcgtgagg ttccgcgcat gaacgagcgc gccgggaagt tcctttacgg
     8881 ctcgttcttc gccgtcgttc tccccgcgct cctcgtcctc tgggcgcgcg cggccggccc
     8941 gaacgtcggg ctcccggccg tggagtcgcc ggtcgggtgg ggcgtcgccg ccttcgggct
     9001 cgggctcctg ctgtgggcga cggcggccct cgcggtcttt gggcgcgggc tcccgatgaa
     9061 cgcgtacccg ccgccggtct tcgtcgggcg cggcccgtac gccgtcgtcg cacacccgat
     9121 ctacctcggg ttcgtcctcg tgtgcgcggg ttgcgcgatc ggctctggct cggcggccgg
     9181 gctctggctc gtcacgcccg cggttgccct cggctcggcg gcgctcgtcc tcggttacga
     9241 gggcccggag ctcgacgtgc gcttcggccc
//
LOCUS       DAOMFY010000143         9230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2281_length_9230_cov_7.053406, whole genome shotgun sequence.
ACCESSION   DAOMFY010000143 DAOMFY010000000
VERSION     DAOMFY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2281_length_9230_cov_7.053406"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PLB01_11945"
     CDS             <1..968
                     /locus_tag="PLB01_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78056.1"
                     /translation="SDARSSTRRSRPSTSSRPSNPTSRPRRTCAAGSCPSGAISPERA
                     AASSAPRPGAAPEGVGFAAPFDPKLGRKIRVYDGEAFRRALGDVPADASGPLLDVRER
                     ALAGALRARFPLPGVTLISKWEETVAWLTFAESVQSARVAEKVSRRLESSAPDLARLL
                     LAAGKMDDLKALDRRLTDASHRLEALEPDPAKARRLRAAALAVRRLVGNGRPPFPQEA
                     RVSKKPGAAVVRIEGDIGALSLVVVENEGLPAGRSRRVLASPLLPVPGSLAVTRNGRA
                     ATWLEAETPTRLARVALALDGSGPVAVPTPAASAKGKKVKSRDHE"
     gene            complement(927..1553)
                     /locus_tag="PLB01_11950"
     CDS             complement(927..1553)
                     /locus_tag="PLB01_11950"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQQ78057.1"
                     /translation="MVKRGTGRGNARRRAPKPPSPSKKSSGAPRTSPSVWPLLARRTL
                     GDFKVFSVRGERTRSPRSGKPHDFFVLECTDWANVVALTDSREVVLVRQFRAGTRSVT
                     LEIPGGSVEKGESAAAAVRRELREETGYAAGSWTRIGVVHPNPAIQGNRCFTYLATGC
                     RRVGALMPDEGEDLAVELVPLRRIPDLIRKGRITHSLVIAAFHFLSLR"
     gene            complement(1587..2342)
                     /locus_tag="PLB01_11955"
     CDS             complement(1587..2342)
                     /locus_tag="PLB01_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQQ78058.1"
                     /translation="MNTEEQNLALFIDIDNLLIGLRESGHPKFDVKLLISRLLEKGKI
                     VYKRAYADWNRYLEYKRAFHEAAFELIDIPQHRMTGKNSADIRMVVDAMDLASNKAHI
                     TTFVIGSGDSDFSPLVSKLKENNKQVLGFGVKGSTSELLIDNCDEFLYYEDLVREKTK
                     APSLQGLPEKTAEAFSLLIDSMLALKRENKEVLWGSMVKQTMQRKYPSFNESYYGFRA
                     FSELLEAAEKAKVITLKRDAKSGSYIVTGFGNT"
     gene            2643..3650
                     /locus_tag="PLB01_11960"
     CDS             2643..3650
                     /locus_tag="PLB01_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HQQ78059.1"
                     /translation="MSAAPLLYDLAGRGTVVLTGKDRATFLHGLVTNDVRKLAPGQGC
                     AAAFLTAKGRLLAECVVLCEAERVVLDTPPELSATVESLLRTYLVFNDVTITNETEET
                     AVLHLTGEAEGGGKEEEDFLRRVIAPGAAASTPREAHAHAPAAFTFKEISSSALPLLL
                     VRENRTGVPGWDVRVPSSLSNQISSSLLSAGARAATEDEFESRRIAAGIPLWGAELTE
                     AVLPDEAGLRERGFIADNKGCYVGQEIVARIKTYGHVNRKLVRLVVFGGVPRPGEEIF
                     FEGEKAGSLTSVAPAAGGGAAALAYVKRERAGAGTALTIASLAGNLPASVAPAAPVPL
                     G"
     gene            3643..4584
                     /locus_tag="PLB01_11965"
     CDS             3643..4584
                     /locus_tag="PLB01_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQQ78060.1"
                     /translation="MAEPLPSGRPRDFRPYLALLAVQLCFGSFPVFGKLALAETAPLV
                     LAAFRAAAGAAALTLLARVLDPNGTFDAKDRRKLMVLSFFGIVANQILFIFGLSKTTA
                     TNATLLTAMTPILTLAVGSVLSEQHTPRRRLLGIPVALAGLLWLLDVSRLDFGDRTFA
                     GNALIFTNCLCYSVFLVLSRDILRRHSAVRVTAAVFRYAALPIVLVAIPDLLSFRPAA
                     LTSRAWTGVAGTIVFATIVAYALNAWGLARTSPSTAAMFMYVQPLVAMTLARVVLGES
                     PSPRTAAAAVLIFAGIALATWPSRGPGAAPAGSPARR"
     gene            complement(4538..6190)
                     /locus_tag="PLB01_11970"
     CDS             complement(4538..6190)
                     /locus_tag="PLB01_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQQ78061.1"
                     /translation="MNERKVLIVDDDEDILLIVQTLLGNAGYATVVARNGKEGIDLAA
                     AVQPDVILLDVTMPQVSGWEVCATLKNLPETASIPIVMLTVKSEIKDLITGMQVGADD
                     YITKPFTKRHLLETVERLLEPREGPRSAFLPKESGDVRTRNLLFDSVTGLPTIPVIVD
                     ALREWLLVDQEVGVLFVDFENYAHIEDFYGWEVFDDVLREASRALKRLLGTLFSAEDL
                     IAVNRPSGSEFYVFLSIPPGLRSEEALERLQRKARQVEESLRTQLNEKFRDRIQRKIA
                     LFVGWSKLLHRPQVRLERLVYRSLREAISMASTKEGERAALLREQFKDILVHRQIGTV
                     YQPIVDLATGSVYAHEALSRGPAESPFENPEVLFDYALKSDQVWALEKICMQFSAERF
                     HGKRDGRLFVNVETELIHDLKLRGHEVLQPLLAIRSGVVLEITERAAIRDYELFRESI
                     QVLRGLGFKIAIDDAGSGYASLQSIAELKPHYLKISNYLVTGLHNDSIKADVVEMLVR
                     LASRIDAATVAEGIETEEELEAVKRLGVTYGQGYLLGRPLAL"
     gene            complement(6214..6528)
                     /locus_tag="PLB01_11975"
     CDS             complement(6214..6528)
                     /locus_tag="PLB01_11975"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQQ78062.1"
                     /translation="MMRDRDVDESGGWNLSVRRFLVTGRVQGVGYRAFAARAARALRL
                     TGGAANLDDGRVEIVAKGPQHALDRLEAALAEGSRIARVDRVDVEALAALPTDASWDV
                     DF"
     gene            complement(6525..7220)
                     /locus_tag="PLB01_11980"
     CDS             complement(6525..7220)
                     /locus_tag="PLB01_11980"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQQ78063.1"
                     /translation="MTAEDSRVQREARALAADRRALVERLRAAGLVSDPRVMDALAAV
                     PRHLFVPAALAGEAYGDHALPIGHGQTITQAANVARSAELAALSPGARVLEIGTGSGY
                     QAAVLARLARWVFTLERIAELARGAQALLKGLGVANVSVKVFDGSYGWSEHAPYDAIV
                     VAAAAPEVPETLVAQLTPTGRLVVPVGAPGRQRLLVVKRLPNGRVRTEDAGEVAYVPL
                     VGRFGFAAKEPGR"
     gene            complement(7217..7993)
                     /gene="surE"
                     /locus_tag="PLB01_11985"
     CDS             complement(7217..7993)
                     /gene="surE"
                     /locus_tag="PLB01_11985"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQQ78064.1"
                     /translation="MKALISNDDGIHSEGLAALQRAAQAAGFDTYVVAPDREQSASSH
                     ALTMHRPLRVRQKDERTWIVDGTPTDCVNIALCSILKADPPDFVFSGINAGPNLGDDV
                     TYSGTVACAFEGTLLGVTSIAFSLDYVRDAVGTVDWSNAEAAARDLIQWAKDHPPAKD
                     TLWNVNVPAGAPKGFRATRMGRRRYGENIVEKIDPRGKPYYWIGGTFVDTKAEGTDLL
                     AVAEGYVSVTPLHMDMTDYRALADLDGLALKLGPRGETKG"
     gene            complement(7990..8433)
                     /locus_tag="PLB01_11990"
     CDS             complement(7990..8433)
                     /locus_tag="PLB01_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQQ78065.1"
                     /translation="MTPAAASGAPAVRITTHAHFSAAHRLHTEGRDEEWNRRVFGKCN
                     NPHGHGHTYGLEVTVEGPVQPETGWVMDFGDLKRVVAERVVKRCDRRNLNVDVPFLAG
                     VNPTAENIAVRIWEELAPAVAPARLVRVVLHETERNKVVYEGPGA"
     gene            complement(8430..8831)
                     /locus_tag="PLB01_11995"
     CDS             complement(8430..8831)
                     /locus_tag="PLB01_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78066.1"
                     /translation="MPGAIENGQRIYLRGGHEGDPLSFSVRWFVPAGFDAGGASEVQS
                     LAHGKQELTSCLTLLGVSALAVMVGDVIDGTPVFETRPCTAVAAAEHIERECQRSRGI
                     AYSQTVWVDATAEEVYRRMRLWESARERWGR"
     gene            9147..>9230
                     /locus_tag="PLB01_12000"
     CDS             9147..>9230
                     /locus_tag="PLB01_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQQ78067.1"
                     /translation="MNLILMLIVGGIIGWLASIVMKTNAQMG"
BASE COUNT         1348 a         3234 c         3169 g         1479 t
ORIGIN      
        1 cttccgacgc aaggagttcg acaaggcgct cgcggccatc gacaagttcg aggccaagca
       61 acccgacaag ccgaccgcgg cgaacctgcg cggccgggtc ctgcccctca ggggcgatat
      121 cgccggagcg cgccgcagct tcgagcgcgc ctcggccggg cgcggctccc gagggcgtcg
      181 ggttcgcggc gcctttcgac ccgaagctcg gcagaaaaat ccgcgtctac gacggcgagg
      241 ccttccgccg ggcgctcggc gacgtccccg ccgacgcctc cgggccgctt ctcgacgttc
      301 gcgagcgcgc gctggcgggg gctctccgcg cgcgctttcc gctgcccggc gtcaccctga
      361 tctccaagtg ggaagagacc gtcgcctggc tgacgttcgc ggaatcggtc cagtccgcac
      421 gggtggccga gaaggtctcg cgccgcctcg agagctcggc tccggatctc gcccgtctcc
      481 tgctcgcggc cgggaagatg gacgacctga aggccctcga ccgcaggctg acggatgcga
      541 gccaccggct cgaagcgctc gagcccgatc ccgcaaaggc ccggcgactg cgcgccgcgg
      601 ccctcgccgt gcggcgcctg gtgggtaatg ggcggccgcc gtttcctcag gaggcgcggg
      661 tctcgaagaa gccgggcgcg gcggtcgtgc ggatcgaggg cgacatcggc gccctctccc
      721 tcgtcgtcgt cgagaacgaa ggcctcccgg ccgggcgctc gcggcgcgtc ctcgcgtcgc
      781 ccctgcttcc ggtcccgggc tcgctcgcgg tgacgcggaa cggacgagcc gccacctggc
      841 tcgaagccga gaccccgacc cgcctcgcgc gcgtcgcgct cgcgctggac ggctcgggtc
      901 cggtcgcggt cccgaccccc gccgcctcag cgaagggaaa gaaagtgaaa agccgcgatc
      961 acgagtgaat gcgtgatgcg gcccttccgg atgagatccg ggattcggcg cagcgggacg
     1021 agctcgacgg cgaggtcctc accttcgtcc ggcatgagcg cgccgaccct gcggcagccc
     1081 gtcgcgaggt acgtgaagca gcggttgccc tggatcgcgg ggttcggatg gacgacgccg
     1141 atgcgcgtcc acgagcccgc cgcgtagccc gtctcctccc gcagctcgcg gcggacggca
     1201 gccgcggcgg actcaccctt ctcgacgctc ccgccgggaa tctcgagcgt caccgagcgc
     1261 gtcccggcgc ggaactgccg gacgaggacg acctcgcggg aatcggtgag cgcgacgacg
     1321 ttcgcccagt cggtgcactc taggacgaag aagtcgtggg gcttgccgct gcgcggcgag
     1381 cgcgtgcgct cgccgcggac ggagaagacc ttgaaatcgc caagcgtgcg cctcgcgagg
     1441 agaggccaga ccgacggtga cgtccgagga gcaccagaag atttcttgga aggtgacggg
     1501 ggcttcggcg cgcgtcgacg cgcgttgcca cgtccggtgc ccctcttaac cattcaaaga
     1561 aatcttcttc tctttcttct tttcgcctac gtgttcccga agccggtcac gatgtacgag
     1621 ccgctcttgg cgtcgcgctt gagggtgatg accttcgcct tctcggcggc ctcgagcagc
     1681 tcggagaacg cgcggaaccc gtagtagctc tcgttgaacg acgggtactt ccgctgcatc
     1741 gtctgcttga ccatcgagcc ccagaggact tccttgttct cgcgcttgag ggccagcatg
     1801 gaatcaatga ggagcgaaaa cgcctcggcc gtcttctcgg gcagaccctg cagggacggg
     1861 gccttcgtct tctcgcggac gaggtcctcg tagtagagga actcgtcgca gttgtcgatc
     1921 aggagctcgg acgtcgaacc cttcacgccg aagccgagga cctgcttgtt gttctccttc
     1981 agcttcgaga cgagcgggga gaagtcggag tcgcccgagc cgatcacgaa cgtcgtgatg
     2041 tgggccttgt tcgacgcgag gtccatcgcg tccacgacca tccggatgtc ggcggagttc
     2101 tttcccgtca tgcggtgctg cgggatgtcg atgagctcga acgcggcctc gtggaaggcg
     2161 cgcttgtact ccaggtagcg gttccagtcc gcataggcgc gcttgtagac gatcttgccc
     2221 ttttcgagga ggcgcgagat caggagcttg acgtcgaact tcgggtggcc ggactcgcgg
     2281 agcccgatca agaggttgtc gatgtcgatg aaaagggcaa ggttctgttc ttcggtattc
     2341 atggcggaag aagggggcgg accgccgact ccctcgccga accccgcggg cacccagccc
     2401 ggggcgaacg gaagggccgc cacggccgcc attgcggtcg gcgcaggggg aatgggtgaa
     2461 ggctccgcct cggggaacgg caggtcggtg gcgtccgcgg cgggtttctt ggcggttgtg
     2521 cggccgcgtc cgcctcggcg gcgccgccgg acgccgcccg ctttcggggc ggcttcgtgt
     2581 tcggtcacgg gaccgcattg tcgcacggat cggcggcccg cgccgggtgc gacaatcggc
     2641 gcgtgagcgc cgcgcccctt ctctacgacc tcgcgggccg cggcaccgtc gtcctgaccg
     2701 gcaaggaccg cgcgacgttc ctccacggcc tcgtcacgaa cgacgtgagg aagctcgccc
     2761 ccgggcaggg ctgcgcggcg gcgttcctga ccgcgaaggg ccggctgctc gcggagtgcg
     2821 tcgtcctctg cgaagcggag cgcgtcgtcc tcgacacgcc gcccgagctg tcggctacgg
     2881 tggagagcct cctcaggacg tacctcgtct tcaacgacgt gacgatcacg aacgagacgg
     2941 aggagacggc tgtcctccac ctcacgggcg aagcggaagg aggcgggaaa gaagaagaag
     3001 actttcttag aagggtaata gcgcctggcg ccgccgcgtc cacgccgcga gaggcgcacg
     3061 cacacgcgcc ggctgcattc accttcaaag aaatctcctc ttcggctctt cctctcctcc
     3121 tcgtccgcga gaaccgcacg ggcgttcccg gctgggacgt ccgcgtcccc tcttcccttt
     3181 caaatcaaat ctcctcttct cttctctcgg cgggagcgcg ggccgccacg gaggacgagt
     3241 tcgagagccg tcgcatcgcc gccggcattc cgctctgggg cgccgagctg acggaggccg
     3301 tcctgcccga cgaggccggg ttgcgcgagc gcggcttcat tgcggacaac aagggctgct
     3361 acgtcggcca ggagatcgtc gcgcgcatca agacgtacgg acacgtgaac cgcaagctcg
     3421 tccggctcgt cgttttcggc ggtgtcccgc ggcccggaga agagattttc tttgaaggtg
     3481 aaaaggccgg cagcctcacg agcgtggcgc cggcggcggg cggcggcgca gccgcactcg
     3541 cgtacgttaa gagagagcgt gccggggccg gcacggcgtt gaccatcgcc tcactcgcag
     3601 ggaatctccc cgcgtccgtc gcacctgccg cgcccgtgcc gcttggctga gccccttccg
     3661 tccgggcgcc cgcgggactt ccgcccgtac ctcgcgcttc tcgccgtcca gctctgcttc
     3721 ggcagcttcc ccgtcttcgg gaagctggcg ctcgcggaga ccgcgcccct cgtcctcgcg
     3781 gcgtttcgcg ccgccgcggg cgccgccgcg ctgacgctcc tggcgcgggt cctcgacccg
     3841 aacggcacgt tcgacgcgaa ggaccggcgg aagctgatgg tcctctcgtt cttcgggatc
     3901 gtcgcgaacc agatcctctt catcttcggc ctgtcgaaga cgacggccac gaacgcgacg
     3961 ctcctgaccg cgatgacgcc gatcctgacg ctcgccgtcg gctccgtgct ctccgaacaa
     4021 cacacaccgc gcagacgact cctcgggatc cccgtcgccc tcgccggcct cctgtggctc
     4081 ctggacgtgt cccgcctcga tttcggcgac cggaccttcg ccggcaacgc cctgatcttc
     4141 acgaactgcc tgtgctactc ggtctttctc gtcctctcgc gcgacatcct gcggcgccac
     4201 tcggcggtcc gcgtcaccgc ggccgtcttc cggtacgcgg ctctcccgat cgttctcgtc
     4261 gccatccccg atctcctgag cttccggccc gcggccctga cgtcccgcgc gtggacggga
     4321 gtcgccggca cgatcgtctt cgcaacgatt gtggcctacg ccttgaacgc gtggggcctc
     4381 gcacggacga gcccgtcgac ggccgcgatg ttcatgtacg tgcagcccct cgtcgccatg
     4441 acgctcgcgc gcgtcgtcct cggcgagtcc ccgtcgccgc gcacggcggc ggccgcggtc
     4501 ctgatcttcg cgggaatcgc gctcgcgacg tggccgtcta gagggccagg ggccgcccca
     4561 gcaggtagcc ctgcccgtag gtgacgccga gccgcttgac ggcctcgagc tcttcctcgg
     4621 tctcgatgcc ctcggcgacg gtcgccgcgt cgatgcgcga ggccagccgg acgagcatct
     4681 cgacgacgtc ggccttgatc gagtcgttgt ggaggccggt cacgaggtag ttcgagatct
     4741 tcaggtaatg cggcttgagc tccgcgatcg actgcaggga cgcgtagccg gagcccgcgt
     4801 cgtcgatcgc gatcttgaag ccgagcccgc gcagcacctg gatgctctcg cggaagagct
     4861 cgtagtcgcg gatggcggcg cgctcggtga tctcgaggac gacgccgctc cggatcgcga
     4921 ggagcggctg gagcacctcg tgcccgcgca gcttcaggtc gtggatgagc tcggtctcca
     4981 cgttcacgaa cagcctgccg tcgcgcttgc cgtggaagcg ctccgccgag aactgcatgc
     5041 agatcttctc gagcgcccag acctggtccg acttgagcgc gtagtcgaag agaacctctg
     5101 ggttctcgaa gggagactcg gccggcccgc gggagagggc ctcgtgcgcg tagacgctcc
     5161 ccgtcgcgag atccacgatc ggctggtaca cggtgccgat ctgccggtgg acgaggatgt
     5221 ccttgaactg ctcgcgcagg agggcggcgc gctccccttc cttcgtcgag gccatcgaga
     5281 tcgcctcgcg aagcgagcgg tagacgagcc gctcgaggcg gacctgcggc cggtggagga
     5341 gcttcgacca cccgacgaag agcgcgatct tccgctggat gcggtcccgg aacttctcgt
     5401 tgagctgggt gcggagcgat tcttcgacct gccgcgcctt gcgctgaagg cgctccagag
     5461 cctcctcgct ccggaggccg ggcggaatcg acaggaagac gtagaactcg gagcccgacg
     5521 gccggttcac cgcgatcagg tcctcggccg aaaagagcgt cccgaggagg cgcttcaggg
     5581 cacgggacgc ctcgcgcagg acgtcgtcga agacctccca gccgtagaag tcctcgatgt
     5641 gggcgtagtt ctcgaagtcg acgaagagga cgccgacctc ctgatccacg agcagccact
     5701 cacgcagcgc gtccacgatc acgggaatcg tcgggaggcc cgtgacgctg tcgaagagga
     5761 ggttcctggt ccggacgtcg cccgactcct tcggcaggaa cgccgagcgc ggtccctcgc
     5821 gcggctcgag gaggcgctcg acggtctcga ggaggtgccg cttcgtgaac ggcttcgtga
     5881 tgtagtcgtc ggcgcccacc tgcatgcccg tgatcaggtc cttgatctcg gacttcaccg
     5941 tcagcatcac gatcgggatg gacgccgtct ccgggaggtt cttgagcgtc gcgcagacct
     6001 cccagcccga gacctgcggc atcgtgacgt cgaggaggat cacgtcgggc tggacggccg
     6061 cggccaggtc gatgccttcc ttgccgttgc gggcgaccac cgtcgcgtac ccggcgttgc
     6121 cgaggagcgt ctggacgatg aggaggatgt cctcgtcgtc gtcgacgatc aggacctttc
     6181 gttcgttcac ggcgccccga tcttaaacgc ccgtcagaaa tccacgtccc acgaggcgtc
     6241 cgtgggcagc gccgcgaggg cctcgacgtc cacgcggtcg acgcgcgcga tgcgcgatcc
     6301 ttcggccagc gcggcctcca ggcggtccag ggcgtgctgc ggccccttcg ccacgatctc
     6361 cacgcggccg tcgtcgagat tcgcggcgcc gcccgtcagc cgcagtgcac gcgccgctcg
     6421 ggccgcgaac gcccggtagc cgacgccctg gacgcggccg gtcacgagga accggcgcac
     6481 ggagaggttc cacccgccgg actcgtccac gtcgcggtcg cgcatcatcg cccgggctcc
     6541 ttcgccgcga agccgaacct cccgacgagc ggcacgtagg cgacctcgcc ggcgtcctcc
     6601 gtgcggacgc gcccgttcgg aaggcgcttg acgacgagca ggcgctggcg gcccggcgcc
     6661 ccgaccggta cgacgagccg tcccgtgggc gtgagctgcg caacgagcgt ctccggcacc
     6721 tcgggcgcgg ccgcggcgac gacgatcgcg tcgtacgggg cgtgttcgct ccagccgtag
     6781 gacccgtcga agaccttcac ggacacgttc gcgacgccga gccccttgag gagggcctgc
     6841 gcgcccctcg ccagctcggc gatgcgttcg agcgtgaaga cccaccgcgc gaggcgcgcg
     6901 aggacggccg cctggtagcc ggaccccgtc ccgatctcga ggacgcgcgc gccgggcgac
     6961 agcgccgcga gctcggccga ccgggccacg ttcgcagcct gcgtgatcgt ctgcccgtgg
     7021 ccgatcggaa gcgcgtggtc gccgtacgcc tcgccggcga gggccgccgg cacgaagaga
     7081 tggcgcggca cggcggcgag cgcgtccatg acgcgcggat cggacacgag gccggccgcg
     7141 cgcaggcgct ccacgagggc ccgccggtcc gcggcgaggg cccgggcctc gcgctgcacc
     7201 cgggagtcct ccgccgtcac cccttcgtct ccccccgcgg cccgagcttc agggcgaggc
     7261 cgtccaggtc ggcgagggcg cggtagtccg tcatgtccat gtggagcggc gtgacggaga
     7321 cgtacccctc cgcaaccgcg aggaggtcgg tcccctcggc tttcgtgtcc acgaacgtcc
     7381 cgccgatcca gtagtacggc ttgccgcgcg ggtcgatctt ctcgacgatg ttctcgccgt
     7441 agcggcgccg tcccatacgc gtcgcccgga agcccttcgg cgctcccgcg ggaacgttca
     7501 cgttccagag cgtgtccttc gccggcgggt ggtccttcgc ccactggatg aggtcccgcg
     7561 cggccgcctc cgcattggac cagtccaccg tgccgaccgc gtcgcgaacg tagtccagcg
     7621 agaacgcgat cgacgtgacg ccgagaagcg tgccctcgaa cgcgcatgcg accgtcccgg
     7681 agtacgtcac gtcgtcgccg aggttcgggc cggcgttgat cccggagaag acgaagtccg
     7741 gcgggtccgc cttcaggatc gagcagagcg cgatgttgac gcagtcggtg ggcgtcccgt
     7801 cgacgatcca ggtccgctcg tccttctggc gcacgcgcag ggggcgatgc atcgtgagcg
     7861 catgggacga ggcgctctgc tcgcggtcgg gcgccacgac gtacgtgtcg aaccccgcgg
     7921 cctgcgcggc gcgctggagc gccgcgagcc cttcgctgtg gatgccgtcg tcgttggaaa
     7981 tgagggcctt cacgctccgg gcccctcgta gaccaccttg ttgcgctcgg tctcgtggag
     8041 gacgacgcgc acgagccgtg cgggggcgac cgcgggagcg agctcctccc agatcctgac
     8101 ggcgatgttc tcggccgtcg ggttcacgcc ggcgaggaac ggaacgtcga cgttcaggtt
     8161 ccgccggtcg cagcgcttca cgacgcgctc ggccacgacc ctcttgaggt ccccgaagtc
     8221 catgacccag cccgtctcgg gctggaccgg tccttccacg gtgacctcga gcccgtacgt
     8281 gtgcccgtgc ccgtgcgggt tgttgcactt gccgaagacg cggcggttcc actcctcgtc
     8341 tcgcccctcc gtgtggaggc ggtgagccgc ggagaaatgc gcgtgcgtcg tgattcggac
     8401 ggccggggcg ccggacgcgg ccgccggggt catcggcccc accgctcgcg cgcgctctcc
     8461 cagaggcgca tccggcggta gacctcctcg gcggtcgcgt ccacccagac cgtctgtgag
     8521 tacgcgatcc cgcggctcct ctggcactcg cgctcgatgt gctccgcagc agccaccgcc
     8581 gtgcacggcc gcgtctcgaa gaccggcgtc ccgtcgatga cgtccccgac catcacggca
     8641 agcgccgaga cgcccagcag ggtcaggcac gacgtcagct cctgcttgcc gtgtgcgagg
     8701 gactggactt cggaggcgcc gcccgcgtcg aatcccgcgg ggacgaacca gcggacggaa
     8761 aacgagagcg gatcgccctc gtgtcccccc cggagataaa tcctctgtcc gttctcgatg
     8821 gcgccgggca tgcgcggagt ttacgagacg aggccctttt cgcgcgcccc ttgactcatc
     8881 gcgttctagg acctacttct tggatggccc tgcgcgccgg tgacggcggg gttggacgga
     8941 gggaggacga aggcaggcga aagccggttc ccgacgaacg acggccgacg gtcgacgtcg
     9001 tttcaaggac acgcgccgat tggcgcaagc caacaaggaa tcctttcgat ccccccgacg
     9061 cggggcgtag cgagcccgag ggaaccggtg ccctcgggct catgtatttt cgggcgtact
     9121 ctctcggcga gtcgaaggag gacgggatga acctgatcct gatgctgatc gtcggaggaa
     9181 tcatcggctg gctggcgagc atcgtcatga agacgaacgc ccagatgggc
//
LOCUS       DAOMFY010000144         9201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2290_length_9201_cov_9.588782, whole genome shotgun sequence.
ACCESSION   DAOMFY010000144 DAOMFY010000000
VERSION     DAOMFY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_9201_cov_9.588782"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2108
                     /locus_tag="PLB01_12005"
     CDS             <1..2108
                     /locus_tag="PLB01_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQQ78068.1"
                     /translation="TKLGYDEHGRLLKSVDAANNTTTYGYTRDVLTSITDANDNTTTY
                     HYDPLKRLDKTTFPDGKFETYTYYADGLLKTKTDRMGQTLTYEYDAFKRLKKKIYPNP
                     AQTITYTYEGQKLKQVDDLYASPAETHTFGYDNSYRVQQNVQSTRGTLTYTYTPDDRV
                     DTMTIAGTPNVTTAHTYYADGSLKTIVWSPQAGQFKYDYTPRGQYQTVTFPNGQTRSY
                     SYDDQGRLTSLANALGATNIATYAYGYDVDWASGQNTMLGQRVSMTATVPSQGFNGAQ
                     TKYSYDPLYQLKKAEYPAAAPFNSEVHQWTYDAIGNRLTNQVNAAIQTYTYEKIGANP
                     KNAQKLLGDGVNTYAYDFNGSQTSRTGFGFVNDPDNRLASINGGTTAMYTYDYQGRRT
                     SKTVAGVTTKYLYDRLNLLSETTAGATTRYAFGPSIDEPLAMYASGAVSYLNADGLGS
                     VVATNSTAGTVTHGTVFDAWGVTKADTGTRTHSFTYTGREVGEAGLQHYRERWQYPST
                     GRFTQEDPLGIYDGPSLYLYVRGQPTQLFDPTGLVSEGECLAACYADAFANYGVGFLP
                     YAKAITAAWERSGHSLNFFQDFLGVDKIVSSSPTRVAAAAGGVAKSIAKGTYDNLGGK
                     EALQRAEDIATRKGFSGRSAKEQAKVLERLGKLRAAARQLGRLGTIFNILNAIDLFND
                     LDNCNTKCEACGWADPISK"
     gene            2178..2861
                     /locus_tag="PLB01_12010"
     CDS             2178..2861
                     /locus_tag="PLB01_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78069.1"
                     /translation="MLIRLCLVVAGGFLGVFIGGNGNETIGFLLGAALTGAGIWVQTR
                     RDRADDEAYFAALRRGERPKPAYELPAWVPKALLAVALVNVVVSLLLWATPEVVRRMT
                     SVRTIRGASLWYGALVVACFWVINQARMAPLVTAPSAIAIWSVRGIVLGGLLAAATRA
                     AMLTSVSRSDVAGLIYLGIVLFVMWSIAAAVVLLGASAFFTERVKNVLENPFARRGRP
                     DVRINGSSK"
     gene            complement(3066..3893)
                     /locus_tag="PLB01_12015"
     CDS             complement(3066..3893)
                     /locus_tag="PLB01_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78070.1"
                     /translation="MLTLYFDTNVFDALYRRGPGCPEGLELQRAVKVGRLRIAISLDV
                     IDETAEAALSDWPMALGRLNLIKDLYDARFEVLPAGTLLRNGIVETLTDARPSSPFSE
                     DPMDIRALCDLLSDRLTDFTKLVNEQRAKKARYAETLSACRNDVRAGLAEMEWPKVRF
                     AHHLEEADWITQAAVDHFFGPGALPKEVLGKLLSSRPVRMHRDVLFSMYYAQSVEGRA
                     PKDSDLVDVHHAITASAAHGIVTEDGPLRGILRRATVPGFQVLSLDEVVGSLRLSTN"
     gene            complement(4170..6404)
                     /locus_tag="PLB01_12020"
     CDS             complement(4170..6404)
                     /locus_tag="PLB01_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQQ78071.1"
                     /translation="MQNLAILLASLAAVGLGIYLFLLRRKRRQFRDTQILKTSIAKEL
                     HLPPSLHPVIDPNTCIGSLSCIKVCPEGDILGIVDGRAALVVAANCIGHSRCELECPV
                     GAIRLVFGTSERGLDLPEVDEFFETNRAGVHIVGELGGMGLIKNAVRQGLQVGRHFKG
                     IVKPLAERQVHATIVGAGPAGIAAALAMKEAGLSFVLLDQGTSLGGTIANYPRQKLVM
                     TETVDLPFYGKFGKPLISKEALMEMFAEALRKAAIKIYHGVKVSGINGEDGAFEVLTE
                     RGKIRSQKVVLAIGRMGTPRKLEVPGEDLPKVTYLLLHPEQYAGKKVLVVGGGDSALE
                     IAQMVAAEKGTEVTISYRSPAFGRAKQRNKDNIETLIRDGRVKALMSSNVLSIHPDKV
                     VLDVAGTTTEIPNDYVVINVGGVLPTEFLKSLGVSIQRHVGQAVEKPKRKKTVSTTSR
                     VITDETAEERQRIRLGTLLAFAGLVILAGLWVVGRNYYWLPPAQRAYSPLHKALKPGG
                     AWGHGIGVVATVVMLSNFLLPLRKHWRRLKGRKSIRHWLTFHVFVGVMSPLVILFHSA
                     FGSNNMVATLCYSSLICVVSTGLVGRFLFGLVPIAGDHYLEMTELRSRMEQLRGSLNP
                     LLEKVQRPQDIHWLLRKATSEPAEGIGFFRALVTFPVDLFRVRLSLVSCRSHFATRDD
                     YHEFSTMITRLLRLKRQASVFRTAKAVMTYWRGFHVVLAVFLVLVITIHIATAWYLGY
                     RWIF"
     gene            6529..6951
                     /locus_tag="PLB01_12025"
     CDS             6529..6951
                     /locus_tag="PLB01_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78072.1"
                     /translation="MEPSRILDEILKDERLSCGAVIDELGRVLTRGGNFDSFPAPSLV
                     SSLLGPTGTPRETYSSLEGQPLPQIWAEGECFAFVDRPAAGIAVVLFGVPVRPRLAFL
                     RPRSGEHEAGSLLEHSKHVSRRLREAFGRKTLEGDEES"
     gene            6964..7215
                     /locus_tag="PLB01_12030"
     CDS             6964..7215
                     /locus_tag="PLB01_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HQQ78073.1"
                     /translation="MSRVVAGKPPASVSESTLSRARHGERVIVRRGKKPVAAVVSIAD
                     LKLLEELEDRYDASISVARLASSREKRIPWAEVRKRAGL"
     gene            7220..7483
                     /locus_tag="PLB01_12035"
     CDS             7220..7483
                     /locus_tag="PLB01_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQQ78074.1"
                     /translation="MAYRLEFVSSAQREFLRLPSGVQERLEPHLNSLEQNPRPRGVKA
                     MAGHRGLFRIRVGDYRILYEIDDASHVVTVTRVRQRGSAYRGL"
     gene            7499..>9201
                     /gene="metG"
                     /locus_tag="PLB01_12040"
     CDS             7499..>9201
                     /gene="metG"
                     /locus_tag="PLB01_12040"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQQ78075.1"
                     /translation="MDGVQRHRSAGYPQQFMAARYLTTPIYYVNDRPHIGHLYTTVVT
                     DALSRYWRLRGEDVRMLTGTDEHGQNIEKVAKKEGVAPIVIADRHAPTFQKLWQTFGI
                     ANDDFIRTTEPRHRKGVEEIIRRLDAAGDLYTASHEGWYCPPCEAFYPEKDLVDGTCP
                     VHGSKLELQKEENVFFRLSKYGDRLLELYDDGVDGRPFVFPDSRLNEVRSFVAAGLKD
                     LSVSRTSITWGLPFPGRPGHVVYVWLDALVNYVTALGLGSGDMSLYERYWPGIHVIGK
                     DILRFHAVYWPAFLMSAGLPVPRQVVGHGWWLRDARKMSKSVGNVVRPDELVAAFGVD
                     ALRWHFLAAMSFGQDANFSDEDFLSVYNADLANGLGNTLSRAVKMASDAFGGKTPGEV
                     CDDNEILAAARDAAAAWTSAFEGYRLQDAASAVRTLVTRIDGYIAAKEPWKKAKAEGV
                     TPALHRIHFATLEGLRIAACLLAPIAPRTATEVFRRLGIEKRAEDVRESDLAWGQLPL
                     GAALPQAPPLFPRADAKEYFASKEKTVTEEKEKDSSKVVSQAGAAGGAPAVAGASPAA
                     AP"
BASE COUNT         1706 a         2910 c         3004 g         1581 t
ORIGIN      
        1 tcacgaagct cggctacgac gagcacgggc ggctcctgaa atccgtcgac gccgcaaaca
       61 acaccacgac gtacggctac acgcgcgacg tcctcacatc catcaccgac gccaacgaca
      121 acaccaccac ctaccactac gacccgctga agcgcctcga caagaccacc ttcccggacg
      181 ggaagttcga gacgtacacc tactacgccg acggcctcct gaagacgaag accgaccgga
      241 tgggccagac cctcacgtac gagtacgacg ccttcaagcg cctgaagaag aagatctacc
      301 ccaaccccgc ccagacgatc acgtacacgt acgaaggcca gaagctcaag caggtggacg
      361 acctctacgc gagcccggcc gaaacgcaca cgttcggcta cgacaattcc taccgcgtcc
      421 agcagaacgt ccagtcgacc cgcggcacgc tcacgtacac ctacacgccc gacgaccgcg
      481 tcgacaccat gacgatcgcc ggcaccccca acgtcacgac cgcccacacg tactacgcgg
      541 acggctccct caagaccatc gtctggagcc cgcaggcggg gcagttcaag tacgactaca
      601 cgccgagggg gcagtaccag accgtgacgt tcccgaacgg gcagacgagg agctatagct
      661 acgacgacca ggggaggctg acgagcctcg ccaacgccct cggcgccacg aacatcgcaa
      721 cgtacgccta tggctacgac gtagactggg cctcgggcca gaacaccatg ctcggccaga
      781 gggtctcgat gacggcgacg gtcccgagcc agggcttcaa cggagcccag acgaagtact
      841 cctacgaccc gctgtaccag ctcaagaagg ccgagtatcc cgccgctgcg cccttcaact
      901 cggaagtcca tcagtggacc tacgacgcca tcgggaacag gctgacgaac caggtgaacg
      961 ccgccatcca gacttacacg tacgagaaga tcggcgcgaa ccccaagaac gcgcagaagc
     1021 tgctcgggga tggtgttaat acgtatgcgt acgacttcaa cgggagccag acgtcacgca
     1081 ccggcttcgg cttcgtgaac gaccctgaca accggctcgc atcgatcaac ggtggtacga
     1141 cggcgatgta cacgtacgac taccagggaa gaaggacgag caagacggtc gccggcgtca
     1201 cgaccaagta cctgtacgac cgcctgaatc ttttatcaga gacgacggct ggcgcgacga
     1261 cgaggtacgc gtttgggccc tcaatcgatg agcctctggc gatgtatgcc tcaggggcgg
     1321 tgagctacct gaacgccgat ggactgggat cggtcgtcgc tacgaactcg accgcgggaa
     1381 ccgtcaccca cggcacggtc ttcgatgcat ggggtgtgac gaaggccgac acggggacga
     1441 ggacgcattc gtttacgtac acggggcgcg aggttgggga ggcgggcctg cagcactaca
     1501 gggagcgctg gcaatatccc tccacaggca gattcaccca ggaagacccc ctcgggatct
     1561 atgacggccc gagtctttac ctatacgttc gtggtcaacc cacgcagctc tttgatccga
     1621 ccgggcttgt ctccgaaggc gagtgcctgg cggcctgcta tgcagatgct tttgcaaatt
     1681 atggtgtcgg tttcttaccg tacgcaaagg ccataacggc tgcctgggag cgctcaggtc
     1741 acagcctgaa tttcttccag gactttctcg gggttgacaa aattgtaagc tcaagcccga
     1801 ctagggtcgc cgcggcggcc ggcggcgttg cgaagagtat cgcaaaaggc acatacgaca
     1861 accttggcgg gaaagaggct ctacagcgcg ctgaggacat tgccacacgc aaggggttct
     1921 cggggaggtc cgccaaagag caagcgaagg tgctcgagag gctcggcaag ctacgcgctg
     1981 cggctagaca gttgggccgc ctcgggacga tattcaatat tctaaatgcg atcgatcttt
     2041 tcaacgacct agacaattgc aacacaaagt gcgaggcgtg cggttgggca gacccaattt
     2101 ccaaatagcc tgacaagcgt ggaactgcgg tacctgagag gagaaaaacg ctctgtgtca
     2161 acaatgaggt acctatcatg ctaatccgcc tttgccttgt cgtcgcgggt gggttcttag
     2221 gagtgttcat cggcggcaac ggaaacgaga cgatcggctt tctactcggc gccgccctca
     2281 ctggcgctgg gatatgggtg cagacgcggc gtgaccgggc tgatgatgag gcgtatttcg
     2341 cggctctgcg ccgaggggag cggccgaagc ctgcttacga gctcccggct tgggttccga
     2401 aagctctctt ggccgtagct ctcgtaaatg ttgtcgtgtc ccttctactc tgggccaccc
     2461 ccgaagtggt taggaggatg acgtcggtcc gtacgattag aggtgcctca ctctggtatg
     2521 gcgctctggt tgtcgcatgc ttctgggtca tcaatcaggc gcgaatggca ccgctcgtga
     2581 cggcaccatc ggcgatcgcc atatggtcgg tccgtggcat cgttctgggt ggtctactcg
     2641 cggccgcaac gagagccgcg atgttgacca gtgtgtcaag gagcgacgtc gcgggcctaa
     2701 tttacctagg aattgttctc ttcgtgatgt ggtctatcgc tgccgcagtc gttctgctag
     2761 gggcgtctgc gtttttcact gagagagtga agaacgtcct cgagaatccg ttcgcacggc
     2821 ggggcaggcc tgatgtgcga atcaacggtt caagcaaata gttcttgaaa gtctgagggt
     2881 cagggctaaa ggtcaaaggt tagggtaggc tctcggcccg ggagggtcgg gggatgtcgg
     2941 ggcttttggg gttagggtct atggtctatt ggggtcaggc ctgtgttatg tggtttagcg
     3001 cggagccccg gatgcggccg atgggccgct gcaggtggtt ctggcgttcg acctcgcgag
     3061 gctcactagt tcgtcgataa cctgagcgat cccacaacct catcgagcga aagcacctga
     3121 aagccaggga cggtcgctcg acggagaatt ccgcgaagag gtccgtcttc ggtcacgatt
     3181 ccgtgtgcgg ccgacgcagt gatcgcgtga tgcacgtcca cgagatccga gtccttcgga
     3241 gcgcgtcctt ccactgactg cgcgtagtac atagagaaca ggacgtcccg gtgcatccgg
     3301 accggcctcg agctcaagag ctttccgaga acctccttcg ggagagcccc cgggccgaag
     3361 aagtggtcga cggcagcctg cgtgatccag tcggcctctt cgaggtgatg cgcgaatcga
     3421 accttcggcc attccatctc tgcgaggccg gcccggacgt cgttcctgca agcactgagc
     3481 gtttccgcgt acctggcttt tttggcgcgc tgttcattca cgagtttcgt gaaatcggtg
     3541 agcctgtccg ataggaggtc acagagggcg cggatgtcca ttgggtcttc gctgaaaggg
     3601 ctcgatggcc tcgcgtcggt cagggtctcg acgatgccgt tgcggagaag agtcccagcg
     3661 ggaaggacct cgaaacgtgc atcgtagagg tccttgatca agttgagacg accgagggcc
     3721 atgggccaat ccgaaagggc tgcctctgcg gtctcatcaa tcacgtcgag tgagatcgca
     3781 atgcgaagcc ggccgacctt gacggcgcgc tgcagttcca aaccctcggg gcagccaggg
     3841 ccgcgccggt acaaggcgtc gaagacgttg gtgtcgaagt agagcgtaag catctcgctg
     3901 gtgggccgat catcgcgcga aagcggccgc ccgcgcctca acaccgaccc ggccggcaag
     3961 gacaccacct atgcctaaag ggtcgttggg agcggtctca atcatctgac ataggtagtt
     4021 cgcgccggtt gcgatcccat ccgccgcgcg ctcctggcgg ctggatggac gtgaccggcg
     4081 ctttccacgg cgttgcgcgg acgacgaagc cgggcggaga cgacgatgga aatgacgaga
     4141 aatagagggc tggtcgattc ggagcgcacc tagaagatcc accggtaccc gagataccag
     4201 gccgtcgcga tgtggatcgt gatcacgagc acgaggaaca cggccagtac gacgtggaag
     4261 ccgcgccagt aggtcatgac cgctttcgcg gtgcggaaca cggaggcctg ccgcttcaat
     4321 ctcaggagcc gcgtgatcat cgtgctgaac tcgtggtagt cgtcccgcgt cgcgaagtga
     4381 gaccggcacg aaacgagcga caggcggacc cggaagaggt ccacggggaa ggtgacgagc
     4441 gcccggaaga agccgatccc ttcggcgggc tccgacgtcg ccttccgcag gagccagtgg
     4501 atgtcctgcg gccgctggac cttctccagc agggggttga ggctcccccg gagctgttcc
     4561 atgcggctcc ggagctcggt catttcgaga tagtggtctc ccgcgatcgg aaccagcccg
     4621 aagaggaagc gccccacgag cccggtcgag accacgcaga tcagcgagct gtagcagagc
     4681 gtcgcgacca tgttgttgga gccgaacgcc gagtgaaaga ggatgacgag ggggctcatc
     4741 acgccgacga agacgtggaa cgtcagccag tggcggatcg acttcctgcc cttcagccgg
     4801 cgccagtgct tgcggagcgg gaggaggaag ttcgagagca tcacgacggt ggcgacgacg
     4861 ccgatgccgt gcccccacgc gcctcccggc ttgagggcct tgtggagcgg tgagtacgcg
     4921 cgctgcgcgg gaggaagcca gtagtagttc cgtcccacga cccacaggcc cgccaggatg
     4981 acgaggccgg cgaaggccag gagcgtgccc agacggatgc gctgccgttc ctcggccgtc
     5041 tcgtcggtga tgacgcggga ggtcgtcgaa acggtcttct tccgcttcgg tttctcgacc
     5101 gcctgcccga cgtgccgctg aatcgaaacg ccgaggctct tgaggaactc cgtggggagg
     5161 acgcccccga cgttgatgac gacgtagtcg ttcgggatct cggtggtggt cccagcgacg
     5221 tcgagcacga ccttgtccgg atggatggac aggacgttgg agctcatcag agccttcacg
     5281 cgtccgtccc ggatgagcgt ctcgatgttg tccttgttcc gctgcttggc cctgccgaag
     5341 gcgggggagc ggtaggagat ggtgacctcc gtccccttct ccgcggcgac catctgggcg
     5401 atctcgagcg cggaatcgcc tcctccgacg acgaggacct tcttccccgc gtactgctcg
     5461 gggtgaagga ggaggtaggt caccttggga aggtcctcgc cgggcacctc cagcttccgg
     5521 ggggtgccca tgcggccgat cgccagcacg accttctggc tcctgatctt cccgcgctcc
     5581 gtcaggacct cgaaggcccc gtcttcgccg ttgatcccgc tgaccttgac gccgtggtag
     5641 atcttgatcg ccgccttcct gagcgcctcg gcgaacattt ccatcagggc ttccttcgag
     5701 atcagcggct ttccgaactt cccgtagaag gggagatcga cggtctccgt catcacgagc
     5761 ttctgccgcg ggtagttggc gatcgttccg ccgagggagg tcccctggtc gaggaggacg
     5821 aaggacaggc cggcctcctt catcgcgagt gccgccgcga tcccggccgg ccccgcgccg
     5881 acgatggtgg cgtggacctg tctttcggcc aggggcttga cgatgccctt gaaatggcgg
     5941 cccacctgca gtccctgccg cacggcgttc ttgatgaggc ccatcccgcc gagctccccg
     6001 acgatgtgaa cgccggcgcg gttcgtctcg aagaactcgt cgacctcggg aaggtcgagc
     6061 ccgcgctcgc tcgtgccgaa cacgagccgg atggcgccga ccgggcactc gagctcgcag
     6121 cgtgagtggc cgatgcagtt ggccgcgacg acgagagcgg ccctcccgtc gacgatgccc
     6181 aggatgtcgc cttccgggca gaccttgatg cacgagaggc tcccgatgca ggtgttcggg
     6241 tcgatgaccg ggtgcagcga aggggggagg tggagctcct tcgcgatcga ggtcttgagg
     6301 atctgcgtgt cccggaactg acgccgcttt cgtcgcagga ggaacaggta gatccccagg
     6361 ccgacggccg cgagcgacgc gaggaggatc gccaggttct gcatcgctta cacctcggtg
     6421 cagccgtcgg attgattcgg ttccggatgg ggtgagcgcc cgaaagacgt gcgccccgtc
     6481 accctattct cgcaggggga ctcgttatgt tacgcggaac ggagcaccat ggagccctcg
     6541 cggatactcg acgagatcct caaggacgag cgcctgtcgt gcggcgccgt gatcgacgag
     6601 ctgggccgcg tcctgactcg cgggggcaac ttcgactcct ttcccgcgcc gagcctcgta
     6661 tcctcccttc tgggtcccac cggaacgcct cgcgagacgt attccagcct cgaggggcag
     6721 ccgctgcccc agatctgggc ggagggcgag tgcttcgcgt tcgtcgaccg gccggcggcg
     6781 ggcatcgccg tcgttctctt cggcgttccc gtgcgccccc gcctcgcgtt tctcaggccc
     6841 cgctcgggag agcacgaggc cggctcgctc ctggagcact cgaaacacgt gagccgccgg
     6901 ctccgggagg ccttcggccg caagacgttg gaaggagatg aggagtcgta gaatgaggac
     6961 gctgtgagtc gcgtcgttgc cggcaagccc cccgcctccg tgagtgaatc cacgctcagc
     7021 cgcgcccgcc atggagagcg cgtgatcgtg cgccgaggaa agaaacccgt cgccgcagtg
     7081 gtatcgatcg cggacctgaa gctcctcgag gagctggaag atcgctacga cgccagcatc
     7141 tccgtcgcgc ggctcgccag ttccagggag aagaggattc cctgggccga ggtccgcaag
     7201 cgcgccggtc tgtaacggcg tggcctaccg gctcgagttc gtctcgtcgg cgcaacgcga
     7261 atttctgcgg cttccgtctg gcgtccagga gaggctcgag ccgcacctga acagccttga
     7321 gcagaacccg aggccacgcg gcgtcaaggc gatggcgggg cacagaggtc ttttccggat
     7381 tcgagtcggc gactatcgga tcttgtacga gatcgacgac gcgagccacg tcgtcaccgt
     7441 gacgcgcgtc aggcagcgag gttcggcgta tcgcgggctc tgatctacat gggctctgat
     7501 ggacggcgtc caacgacacc gctccgcggg ttatcctcag cagttcatgg cggcgcgcta
     7561 cctcacgacc ccgatctact acgtcaacga ccggccccac atcgggcacc tctacacgac
     7621 ggtcgtgacg gacgcgcttt cgcgctactg gcgcctgcgc ggcgaggacg tgcggatgct
     7681 gaccggcacg gacgagcacg gccagaacat cgagaaggtc gcgaagaagg aaggcgtggc
     7741 gccgatcgtc atcgccgacc gccacgcgcc cacgttccag aagctctggc agacgttcgg
     7801 gatcgcgaac gacgacttca tccggacgac cgagccgcgg caccggaagg gcgtcgagga
     7861 gatcatccgc cggctcgacg ccgcgggcga cctctacacg gcgtcacacg agggctggta
     7921 ctgcccgccc tgcgaggcgt tctaccccga gaaggacctc gtggacggca cgtgccccgt
     7981 ccacgggagc aagctcgagc tgcagaagga agagaacgtc ttcttccggc tctcgaagta
     8041 cggcgaccgc ctcctcgagc tctacgacga tggcgtggac ggcaggccgt tcgtcttccc
     8101 cgactcgcgc ctgaacgagg tgcgctcgtt cgtggccgcg ggactcaagg acctttccgt
     8161 ctcgcgcacg tccatcacgt ggggcctccc gttccccggg cgccccggcc acgtcgtcta
     8221 cgtgtggctc gacgcgctcg tgaactacgt cacggcgctg ggcctgggct cgggcgacat
     8281 gtcgctctac gagcggtact ggccgggcat ccacgtcatc gggaaggaca tcctccgttt
     8341 ccacgccgtc tactggcccg cgttcctcat gtcggccggc ctgcccgtcc cgcgccaggt
     8401 cgtcggccac ggctggtggc tgagggacgc gcgcaagatg tcgaagtcgg tcggcaacgt
     8461 ggtgcgcccc gacgagctcg tcgcggcgtt cggcgtggac gccctgcggt ggcacttcct
     8521 cgccgcgatg tcgttcgggc aggacgcgaa cttctccgac gaggatttcc tctccgtcta
     8581 caacgccgac ctcgcgaacg gcctcggcaa caccctctcg cgcgccgtga agatggcgtc
     8641 ggacgccttc ggcgggaaga cgcccggcga ggtgtgcgac gacaacgaga tcctggccgc
     8701 cgcacgcgac gcggctgccg cgtggacgtc cgcgttcgag ggttaccgcc tgcaggacgc
     8761 cgcgtccgcc gttcgcacgc tcgtgacgcg gatcgacggc tatatcgccg cgaaggagcc
     8821 gtggaagaag gcgaaggccg agggcgtcac gcccgcgctc caccgcatcc acttcgcgac
     8881 gctcgagggg ctgcgcatcg ccgcgtgcct cctcgcgccg atcgcgccgc gcacggcaac
     8941 ggaagtcttc cggcggctcg gaatcgaaaa gcgcgccgag gacgtccgcg agtccgatct
     9001 cgcgtggggc cagctccctc tgggcgccgc gctgccacag gcgccgccgc tgttcccgcg
     9061 ggcggacgcg aaagaatatt ttgcttccaa ggagaagacc gtgactgaag agaaagagaa
     9121 agattcttcg aaggtggtgt ctcaggcagg cgccgcgggt ggcgccccgg cggttgcggg
     9181 cgcgagcccc gcggcggcgc c
//
LOCUS       DAOMFY010000145         9177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2301_length_9177_cov_7.188336, whole genome shotgun sequence.
ACCESSION   DAOMFY010000145 DAOMFY010000000
VERSION     DAOMFY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_9177_cov_7.188336"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLB01_12045"
     CDS             complement(<1..840)
                     /locus_tag="PLB01_12045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ78076.1"
                     /translation="MTDLTGRTLGRYRLDSVLGRGGMADVWRATDVKLARTVAVKVIH
                     AAYAKDPHFVERFMREARVVASLEHPNILPVYDFGEEDGMPFLVMPHLAGGTLRDRLN
                     GAPAPLGLAAAWIRQLADALDAAHAAGVLHRDVKPANVLIGKDDRLFLADFGIAKMAE
                     TMTGLTATGVVVGTPLYMAPEQAQGRPASPATDRYALAVIAYEILAGRPPFEGDNPLS
                     LMHQHVSSPAPALSSRVGGLPAGLDAVLAAALSKDPARRPPTCRALADAVAGFLPTGA
                     MPVA"
     gene            complement(879..1436)
                     /locus_tag="PLB01_12050"
     CDS             complement(879..1436)
                     /locus_tag="PLB01_12050"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQQ78077.1"
                     /translation="MSPAESEDWKERLKALGIGRKRGKKKEKREDFLGKEEGGLPAAA
                     SGAPGSEAPRRSSFKEISLRNLQKVESGRRGEDLASSLLEENLLRILDRNVRYKDGEL
                     DIVALDGGTTVFVEVKRRRDSSLGDPAEAVTRTKRLRVVRAARRWLAAHPSRARSVRF
                     DVVAVLGDPPAVTWIKNAFGADGGA"
     gene            1461..1754
                     /locus_tag="PLB01_12055"
     CDS             1461..1754
                     /locus_tag="PLB01_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78078.1"
                     /translation="MSDYLLVHCPECAATLKVDRETGAVLSHETVAHKTKLGSLEEAA
                     RENTRRKERAEDLFAATVEREKNKSSILDKTFREALERAKADPSKPKGIFDDE"
     gene            complement(1761..2147)
                     /locus_tag="PLB01_12060"
     CDS             complement(1761..2147)
                     /locus_tag="PLB01_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78079.1"
                     /translation="MKPAPRIVGPVAPAVYTKEGKSGLYKAVADVDEPLVTEGMKARQ
                     RQLDKKRRAIAMLRQVEVEEAEDRWRHYRHWIYRLVAVLAALGVYAKIQMTYGDKWPV
                     WTVWIFLGTVILTALFWLFWYIDYSD"
     gene            complement(2206..4320)
                     /locus_tag="PLB01_12065"
     CDS             complement(2206..4320)
                     /locus_tag="PLB01_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQQ78080.1"
                     /translation="MIITPTQTTLLGMPGTVLFALVLLSALAFFAYTMSRRVQLLTMG
                     AGLPDDRFDRPWERFVHVLTYGLFQKKMFRDPYAGLYHALIFGGFIVLSVRTLTLVFE
                     GLFPGAAMPFLGPGFWEGYLLLKDVVLVTTLAGVVLALGRRHVFRKERLDPSFDADLI
                     LCLIGFLMVTDLAAGAARFALLEKVGEGTAARAAAAAGAMWEPVTFFLSKQIFSFSSS
                     SLQGIYFGAWWGHLVAILVFANYLPFAKHFHVITALPNLYFAKLDPAGKMATFDIEKA
                     AETERFGVGRIEDFTWKQKLDFMTCTECGRCREICPTHLTGKPLTPKGFTVDLRNTLY
                     AEADDLIDVDSGRGDGAAAARLAERKPLIGGWISEDTIWACTTCRYCEHACPVGITYI
                     DKITDMRRHLVLEKSEFPKEAQTSFNGMERQGNPWNLPAADRGAWAKDLPFPVLTMAE
                     AAEAGGVDVLFWVGCAGSYEERGKKVSQALARLLHEAGVTYAILGSEETCTGDAARRL
                     GNEYLFQTLAQQNIETMNGYGVKKIVTNCPHCFNTLANEYPDFGGKYEVVHGTELVAK
                     LVAEGRVKLTEKIEQTISFHDPCYLGRHNGVYEAPRDILDAIPGLSVKELPRSRDAGM
                     CCGAGGGRMWLDETLGTRINQARYAEIQANGTDAVGVSCPFCMVMLGNAKTETGGTTD
                     AFDVLELAVRALPAHPGASKTA"
     gene            4408..6216
                     /locus_tag="PLB01_12070"
     CDS             4408..6216
                     /locus_tag="PLB01_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78081.1"
                     /translation="MKSRRSVFGTASVALVLLALALASPAPAAAPAGTDLWGGPGAKG
                     TGSNGATFDTTITVSSLAAANGSVDFVAGGLVVATTPFTLASRGAASLATPAALDGKG
                     AFLYHVRSDASVSAWSETYNDTPSGRYSTSFTAFPVSDFLTAGDEAWGGGADASTSTA
                     PGRARTNVGVLCSPLGAQGCNVEWAAFDGGTLVGSAVMFASPGAAAQQALAALIPAAA
                     EKSRLSIRARVSSGSAAPYAVKNDNLTSDGAAIPLSIVRSAFSTAPTINSFTITPLTG
                     CSPLSALATWVTTGADHVNIAGASGDLPPSGSTTITVVATGDVILTAVATSGATATQP
                     KRVTVNPPTDPPTPSPSSIVTAIGQTTTGVLPINVGAVTAVFVTQGSTGSTFTVVGNQ
                     FTYKAGTVIGTDVVRLTSQGFCGPAVATFTANVVDPGTPVVTSFTADPPRGCGATTNV
                     VLAWTTENARSVIIDVAPAPYPYSANSATGITITGSTTANLTAFALFGGSGTATASLT
                     IPVDTQPYAPIVSPNNIIVAPNSPPVFLTVTGVPDATLLRTVLIQNQTGSSFQSTATP
                     GIFAYVPGFRNGTDIVRIFFTNGCGSQYAEFIANVQ"
     gene            complement(6423..7400)
                     /locus_tag="PLB01_12075"
     CDS             complement(6423..7400)
                     /locus_tag="PLB01_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQQ78082.1"
                     /translation="MSNVLVFIEQRDGKLRKSSLEALTLGHSLSKKTSGALGAVVAGS
                     GISSLVPELGAYGAAKVFVADKPELALFSSEGYTAALDAAATAFNPSIVLVSATAMGL
                     DVAARFAARRGVGVLSDVMALDVENGRLVGSRPVYSGKARVTVTAAPSAAIQVATPRP
                     NVFPAAKADAPGAAEAVAIDPVLKIRGKVVKIEAAAAGEIDVAEADKIVTGGRGIKGP
                     ESWPILKALCHELGAALGASRAAVDAGWIGHEHQVGQTGKVVSPSLYIACGVSGAIQH
                     LAGMGSSKVIVAINKDPEAPIFKAATYGIVGDLFQIVPAMTEAAKKMKA"
     gene            complement(7453..8241)
                     /locus_tag="PLB01_12080"
     CDS             complement(7453..8241)
                     /locus_tag="PLB01_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQQ78083.1"
                     /translation="MNHIVCIAYVPDTETKIKVAGDGLSIDEADVKWIVSPYDEFALE
                     GALKAKEASGGTVTAVTFGPARADAGLRECLARGADEAVRVASDGLGQVDSLGVATLL
                     AAAIKALPHDVVWMGNKGVGTDAQVLVPMLAELLDLPHANLVTKMDWTGAGATVRREI
                     EGAQEIVELPTPFVVGATKGLNEPRYASLKGIMAAKKKTIAVKTPADLGVDPAKISGD
                     AASVRWTKLELPPARQAVKLIPADDPAKAATELLRLLREEAKVL"
     gene            8444..>9177
                     /locus_tag="PLB01_12085"
     CDS             8444..>9177
                     /locus_tag="PLB01_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78084.1"
                     /translation="MRRAWPVLLPLLLAAAADAGQVLKFSEEGTRDGKAYRSSLTLRV
                     SAEGVRAEATDFSEPGAPKTVVYLYVAKDARILPIDPPTGPVISAGTIAAVEERARAA
                     GHRRRPGAFSATPLLATQTFGKWTCDSWAIRRPGQTTEIVCLADPKTLGVDATTRANL
                     RKMNALFVPFLNAVRLAGGDPREDTNAYALEGGFPVRTFRSKDGVVELDAQLASVESA
                     DFPPDVFRAPEPPGPAAAPSTPAAPAP"
BASE COUNT         1366 a         3313 c         3082 g         1416 t
ORIGIN      
        1 ggcgacgggc atcgcgcccg tcggcaggaa cccggcgacg gcgtccgcga gcgcgcggca
       61 cgtcgggggc cggcgcgccg ggtctttcga gagcgccgcc gcgaggaccg cgtcgaggcc
      121 cgcggggagg cctcccaccc tcgaggagag cgcgggcgcc ggactggaca cgtgctgatg
      181 catgagggaa agagggttgt cgccctcgaa cggaggccgc ccggcgagga tttcgtacgc
      241 gatgaccgcg agcgcgtagc ggtccgtcgc cgggctcgcc ggccggcctt gagcctgctc
      301 gggggccatg tagagcgggg tcccgacgac gacgcccgtc gccgtgagcc ccgtcatcgt
      361 ctcggccatc ttcgcgatcc cgaagtcggc gaggaagagc cggtcgtcct ttccgatcag
      421 gacgttcgcg ggcttcacgt cacggtggag gacgcccgcg gcgtgcgcgg cgtcgagcgc
      481 gtcggcgagc tggcggatcc acgccgccgc gaggccgagc ggcgcgggcg cgccgttcag
      541 gcggtcgcgc agcgtcccgc ccgcgaggtg cggcatgacg aggaacggca tcccgtcctc
      601 ttcgccgaag tcgtagaccg gcaggatgtt cggatgctcg agggacgcga ccacgcgcgc
      661 ctcgcgcatg aatcgttcga cgaagtgcgg gtccttcgcg tacgcggcgt ggatcacctt
      721 gacggccacc gtccgcgcga gcttcacgtc ggtggcgcgc cacacgtcgg ccattccgcc
      781 ccgcccgaga accgaatcga ggcggtagcg cccgagcgtg cggcccgtca ggtcggtcat
      841 ggggtgctcc gttccgacgt cgtgatcgtg tgcggagttt acgctccgcc gtccgcgccg
      901 aacgcgttct tgatccacgt cacggccggc ggatccccca ggacggcgac gacgtcgaag
      961 cgcacggacc gcgcccgcga cgggtgcgcg gccagccacc tgcgggccgc gcgcacgacg
     1021 cgcaggcgct tcgtgcgcgt cacggcctct gcgggatcac cgagcgacga gtcccggcgg
     1081 cgcttcacct cgacgaagac ggtcgtgccc ccatccagag ccacgatgtc cagctcacca
     1141 tccttgtagc ggacgtttcg gtcgaggatt cggagaagat tttcttcgag tagtgaagag
     1201 gcgaggtcct cgcctcggcg cccgctctcc accttctgga ggttccgaag agagatttct
     1261 ttgaatgatg aacgacgagg cgcctcgctt ccgggcgcac cgctcgcagc tgcgggcagc
     1321 cctccctctt cctttccaag aaaatcttcc ctcttctctt tcttcttccc cctcttccgt
     1381 ccaatgccca aggccttcag ccgctctttc cagtcctcgc tttcggccgg gctcacggga
     1441 agaggataat tctctccgcc atgagcgact acctcctcgt ccactgcccc gagtgcgcgg
     1501 ccacgctgaa ggtcgaccgg gagaccggcg cggtcctgtc ccacgagacc gtcgcccaca
     1561 agacgaagct cggctcgctc gaggaggccg cccgcgagaa cacgcggcgc aaggagcgcg
     1621 ccgaggacct gttcgccgcg accgtcgagc gcgagaagaa caagtcgagc atcctcgaca
     1681 agacgttccg ggaagcgctc gagcgcgcga aggccgatcc gtcgaagccg aagggcatct
     1741 tcgacgacga gtaactcaaa tcagtcgctg tagtcgatgt accagaacag ccagaacagc
     1801 gccgtcagaa tcacggtgcc gaggaagatc cagaccgtcc agaccggcca cttgtcgccg
     1861 taggtcatct ggatcttcgc gtacacgccg agggcggcga gcacggcgac gaggcggtag
     1921 atccagtggc ggtagtggcg ccagcggtcc tcggcctcct cgacctcgac ctgccggagc
     1981 atcgcgatcg cgcggcgctt cttgtccagc tggcgctgcc gggccttcat gccctccgtc
     2041 acgagaggct cgtccacgtc ggcaacggcc ttgtagaggc ccgacttgcc ttccttcgtg
     2101 tagaccgcgg gcgccacggg ccccacgatc cgcggggcgg gcttcacgcc ggtacccgtc
     2161 ggcgggcccc cttttttcgg ttcgtccggc acgttgagac ggagtttacg ccgttttgga
     2221 cgccccgggg tgcgccggga gggcccgcac ggccagttcg aggacgtcga acgcgtcggt
     2281 cgtcccgccg gtttccgtct tcgcgttccc gagcatgacc atgcagaacg ggcacgaaac
     2341 gcccacggcg tcggtcccgt tcgcctggat ctcggcgtag cgcgcctggt tgatccgcgt
     2401 cccgagcgtc tcgtccagcc acatccggcc gccgcccgcg ccgcagcaca tcccggcgtc
     2461 gcggctgcgc ggcagctcct tcaccgagag gcccgggatc gcgtccagga tgtcgcgcgg
     2521 cgcctcgtag acgccgttgt ggcggccgag atagcacggg tcgtggaacg agatcgtctg
     2581 ctcgatcttc tccgtgagct tgacgcggcc ctcggcgacg agcttcgcga cgagctcggt
     2641 cccgtggacg acctcgtact tcccgccgaa gtcggggtac tcgttcgcga gcgtgttgaa
     2701 gcagtgcggg cagttcgtga cgatcttctt cacgccgtag ccgttcatcg tctcgatgtt
     2761 ctgctgcgcg agcgtctgga agagatactc gtttccgagg cggcgcgccg cgtcgcccgt
     2821 gcacgtctcc tcgctcccga ggatcgcgta cgtcacgccc gcctcgtgga ggaggcgcgc
     2881 gagcgcctgc gacaccttct tgccgcgctc ctcgtacgag ccggcgcagc ccacccagaa
     2941 gaggacgtcg acgccgccgg cctccgccgc ctccgccatc gtgaggaccg ggaacgggag
     3001 gtccttcgcc cacgcgccgc ggtccgcggc gggcaggttc cacgggttgc cctggcgctc
     3061 catcccgttg aaggacgtct gcgcctcctt cgggaactcg ctcttctcga ggacgaggtg
     3121 gcgccgcatg tccgtgatct tgtcgatgta cgtgattccg accgggcacg cgtgctcgca
     3181 gtagcggcac gtcgtgcacg cccagatggt gtcttccgag atccagccgc cgatgagcgg
     3241 cttgcgctcc gcgaggcggg ccgcggccgc gccgtcgccg cggccggagt ccacgtcgat
     3301 gaggtcgtcc gcctcggcgt agagcgtgtt gcgcaggtcg accgtgaatc ccttcggcgt
     3361 gagcggcttg cccgtcaggt gcgtcgggca gatctcgcgg caccgcccgc actccgtgca
     3421 cgtcatgaag tcgagcttct gcttccacgt gaagtcctcg atgcgtccga cgccgaagcg
     3481 ctccgtctcg gcggctttct cgatgtcgaa cgtcgccatc ttcccggcgg ggtcgagctt
     3541 cgcgaaatag aggttcggca gcgccgtgat gacgtggaag tgcttcgcga acggcaggta
     3601 gttcgcgaac acgaggatcg cgacgagatg cccccaccac gcgccgaaat agatgccctg
     3661 gagggaagag gaagagaacg agaatatttg cttagaaagg aagaaggtca ccggctccca
     3721 catcgcgccc gcggcagcgg cggcgcgcgc cgcggtgccc tcgcccacct tttccagcag
     3781 cgcgaagcgc gccgcccccg ccgcgaggtc cgtcaccatc aggaacccga tgaggcagag
     3841 gatgaggtcg gcgtcgaagc tggggtcgag gcgctccttc ctgaagacgt ggcgccgtcc
     3901 cagggcgagg acgacgcccg cgagcgtcgt cacgaggacg acgtccttca ggagaaggta
     3961 gccctcccag aagcccggcc cgaggaacgg catcgcggcg cccgggaaca ggccctcgaa
     4021 gacgagggtg agcgtccgga ccgagaggac gatgaacccg ccgaagatga gggcgtggta
     4081 caggcccgcg tacgggtcgc ggaacatctt cttctggaag agcccgtacg tgaggacgtg
     4141 gacgaaccgc tcccacggcc ggtcgaaccg gtcgtccgga aggccggctc ccatcgtgag
     4201 gagctggacg cgccgggaca tcgtgtacgc gaaaaaggca agggcggaaa gcaggacgag
     4261 cgcgaagagg accgtgcccg gcattccgag gagggtggtc tgggtgggcg tgatgatcat
     4321 cgggccgagg atatcgcggg cgctctgcta agataagtcg tggaatctcc gtcctttcaa
     4381 cgcgttcccc gacacgggag taacgagatg aagtctcgcc gaagcgtctt cggaaccgcg
     4441 tccgtggccc tcgtcctgtt ggccctggcc ctcgcctcgc cggcgccggc cgcggccccc
     4501 gccgggaccg atctctgggg cggccccgga gccaagggaa ccgggtccaa cggcgccacg
     4561 ttcgacacca cgatcacggt gtcgtccctg gccgccgcga acggctccgt cgacttcgtc
     4621 gccggcggcc tggtcgtcgc cacgacgccc tttacgctcg cgagccgcgg cgccgcgtcg
     4681 ctcgcgaccc cggccgcgct ggacggaaag ggcgcgttcc tctaccacgt ccgctccgac
     4741 gcctccgtct ccgcgtggtc ggagacctac aacgacacgc cgagcggccg ctactccacg
     4801 tccttcacgg ccttccccgt ctccgacttc ctcacggccg gcgacgaggc ctggggcggc
     4861 ggcgccgacg cctccacgtc cacggccccg ggccgcgccc gcacgaacgt cggggtcctg
     4921 tgcagcccgc tcggcgcgca gggctgcaac gtcgagtggg ccgccttcga cggcggcacg
     4981 ctcgtcggtt cggccgtgat gttcgcctcc cccggagccg cggcgcagca ggcgctcgcg
     5041 gcgctcatcc cggccgccgc cgagaagtcg aggctctcga tccgcgcgcg cgtctcgtcg
     5101 ggctcggcgg cgccgtacgc cgtgaagaac gacaacctca cgagcgacgg cgcggcgatc
     5161 ccgctctcca tcgtgcgcag cgccttctcg acggcgccca cgatcaactc cttcacgatc
     5221 acgccgctca cgggctgctc gccgctctcg gccctcgcga cgtgggtgac gaccggcgcc
     5281 gaccacgtca acatcgccgg cgcctcgggc gacctgccgc cgagcggctc gacgacgatc
     5341 accgtcgtcg cgaccggaga cgtgatcctc acggccgtcg cgacctcggg cgccaccgcc
     5401 acgcagccca agcgcgtcac cgtgaacccg cccaccgacc cgccgacgcc gtcgccctcc
     5461 tcgatcgtga cggccatcgg ccagacgacc acgggcgtcc ttccgatcaa cgtcggcgcc
     5521 gtcacggccg tgttcgtcac gcaggggtcc acgggatcga cgttcacggt cgtcgggaac
     5581 cagttcacgt acaaggccgg gacggtgatc ggcacggacg tcgtgcgcct cacctcgcag
     5641 ggcttctgcg gacccgccgt cgccacgttc acggcgaacg tcgtcgaccc cggcacgccg
     5701 gtcgtcacct cgttcacggc cgatccgccg cgcggctgcg gcgcgaccac gaacgtcgtc
     5761 ctcgcttgga cgaccgagaa cgcccgctcc gtcatcatcg acgtggcgcc cgcgccgtac
     5821 ccgtactcgg cgaacagcgc gacgggcatc acgatcacgg gctcgacgac cgcgaacctc
     5881 accgcgttcg cgctcttcgg cggatccggg acggcgaccg cgtccctcac gatccctgtg
     5941 gacacgcagc cctacgcgcc gatcgtctcc ccgaacaaca tcatcgtcgc accgaactcg
     6001 ccccccgtct tcctcaccgt gacgggcgtg cccgacgcga cgctgctccg cacggttctc
     6061 atccagaacc agacgggcag ctccttccag tccacggcga cgccggggat cttcgcctac
     6121 gtgcccggct tcaggaacgg cacggacatc gtccgcatct tcttcacgaa cggctgcggc
     6181 tcgcagtacg cggagttcat cgcgaacgtt caatagggag agagaaagag attctcttag
     6241 aaggtcaaga gagggcggca gcgccgggtg cccctcgtac gcgacgtcga gcgacgcgcg
     6301 tccggggggc ctttcaattc cgggagccgg tggcgcgcgg cgcgcgagag cgaaggctaa
     6361 agggccccgg aggtccgggg cctgagcgat cgcggtagag gggtacccgg cgatgccggc
     6421 ggctaggcct tcatcttctt cgccgcttcc gtcatcgcag gaacgatctg gaagaggtcg
     6481 ccgacgatgc cgtaggtggc ggccttgaag atgggagcct cggggtcctt gttgatcgcg
     6541 acgatgacct tcgacgagcc catgcccgcg aggtgctgga tcgctccgga cacgccgcac
     6601 gcgatgtaaa gcgacggcga gacgaccttg ccggtctgcc cgacctggtg ctcgtggccg
     6661 atccagcccg cgtccaccgc cgcgcgcgag gcgccgagcg ccgcgcccag ctcgtggcag
     6721 agcgccttca ggatgggcca gctctccggc cccttgatgc cgcggccgcc cgtcacgatc
     6781 ttgtcggcct cggccacgtc gatctccccc gccgcggcgg cctcgatctt cacgaccttg
     6841 ccgcggatct tcagcaccgg gtcgatcgcg acggcctcgg cggcgccggg cgcgtcggcc
     6901 ttcgcggccg ggaacacgtt cgggcgcggc gtcgcgacct ggatggccgc ggacggcgcg
     6961 gcggtcacgg tgacgcgcgc cttgcccgag tacacggggc gcgaaccgac gaggcggccg
     7021 ttctcgacgt ccagcgccat cacgtccgac agcacgccga ccccgcggcg cgccgcgaag
     7081 cgcgcggcga cgtccagccc catcgccgtc gccgagacga gaacgatgga cgggttgaaa
     7141 gccgtggcgg ccgcgtcgag cgcagccgtg tagccctcgg aggagaagag cgcgagctcg
     7201 ggcttgtcgg ccacgaacac tttcgcggcc ccgtaggcgc cgagctcggg gaccagagag
     7261 gagattcctg aacctgccac aacagcgcca agggcgccgc tggttttctt agaaagtgaa
     7321 tggccgaggg tgagcgcctc gagggagctc ttcctcagct ttccatcccg ctgttcgatg
     7381 aagaccagga cgttcgacat gctgtctttc cctcttaacc ttctaagaaa atcttctctt
     7441 ctctcttctt cctcagagga ccttggcttc ttcgcgcagc aggcggagca gctccgtcgc
     7501 cgccttggcc gggtcgtccg ccgggatgag cttcacggcc tgccgcgcgg gcggcagctc
     7561 cagcttcgtc cagcgcacgc tcgcggcgtc gccggagatc ttcgcggggt ccacgccgag
     7621 gtcggcgggc gtcttgacgg cgatcgtctt cttcttggcc gccatgatcc ccttgagcga
     7681 ggcgtagcgc ggctcgttca ggcccttcgt cgcgccgacg acgaacggcg tcggcagctc
     7741 gacgatctcc tgcgcgccct cgatctcgcg gcgcacggtc gcgcccgcgc ccgtccagtc
     7801 catcttcgtg acgaggttcg cgtgcggcag gtcgaggagc tcggcgagca tcgggacgag
     7861 cacctgcgcg tcggtgccga ctcccttgtt gcccatccag acgacgtcgt gcggcagcgc
     7921 cttgatcgcc gcggcgagca gcgtcgccac gccgagcgag tccacctgcc cgagcccgtc
     7981 ggacgcgacg cgcacggcct cgtccgcgcc gcgcgcgagg cactcgcgca gcccggcgtc
     8041 agccctcgcg gggccgaacg tgaccgccgt gacggtgccg ccgctcgctt ccttcgcctt
     8101 gagggcgccc tcgagcgcga actcgtcgta cggcgagacg atccacttga cgtcggcctc
     8161 gtcgatcgag agcccgtcgc ccgcgacctt gatcttcgtt tcggtgtcgg gaacgtaggc
     8221 gatgcagacg atgtgattca tggcgggggg aaactagcat ggattctgcg ctgcgtcacc
     8281 gagaaaatga gccgcggctc attaacgggc aaacgtttgg atgggttcgg cttgcggccg
     8341 ccgcccgccc cgggccgatg agggtgagac tcggcatcgc aggaggcccc gcgatgcggc
     8401 catggaaggc cgaccccggg ttcgcgcccc gtaagatccc gccgtgcgcc gcgcatggcc
     8461 cgtccttctc cccctcctcc tcgcggcggc cgccgacgcc gggcaggtcc tgaagttctc
     8521 cgaggaagga acgcgggacg gaaaggcgta ccgctcgtcc ctcacgctcc gcgtctcggc
     8581 cgagggcgtg cgggccgagg cgacggactt ctcggagccc ggcgcgccga agacggtcgt
     8641 ctacctctac gtcgcgaagg acgcccggat cctcccgatc gacccgccga cgggtccggt
     8701 gatctccgcc ggcacgatcg ccgccgtcga ggagcgcgcg cgcgccgcgg gccatcgccg
     8761 ccgccccggc gcattctcgg ccacgccgct ccttgcgacg cagacgttcg ggaagtggac
     8821 ctgcgactcg tgggccatcc gccgcccggg ccagacgacc gagatcgtct gcctcgccga
     8881 cccgaaaacg ctcggcgtcg acgcgacgac gcgcgcgaat ctccggaaga tgaacgccct
     8941 cttcgtcccg ttcctgaacg cggtccgcct cgcgggaggc gacccgcgcg aggacaccaa
     9001 cgcgtacgcg ctcgagggcg gcttccccgt gcgtacgttc cgttcgaagg acggcgtcgt
     9061 cgagctggac gcgcagctcg cctccgtcga gagcgccgac ttccccccgg atgtcttccg
     9121 cgcgcccgag ccgcccggtc ccgcagccgc gccctccacg cccgcggcac ccgcacc
//
LOCUS       DAOMFY010000146         9169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2303_length_9169_cov_7.227452, whole genome shotgun sequence.
ACCESSION   DAOMFY010000146 DAOMFY010000000
VERSION     DAOMFY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_9169_cov_7.227452"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PLB01_12090"
     CDS             <1..1479
                     /locus_tag="PLB01_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78085.1"
                     /translation="AAPAAPAAAPAERPKRRVVLFVDRLTLGDQRRSARFFGSLKDFV
                     VRTIQPGDEAAVLTFDESLATRVPFTGDTFAVLKALDALAKESARPPAALTGVETSEK
                     LVAEIAQAEREMAQRESGRGGIATATIPTEAVAPQAAPISGTVLAEMRGLAAQEWGRT
                     KNKAAALKAVLSALGGLDGRRVLVVASHRLSRWSGMEYFLTKRLDVDVQVPPEAREFD
                     ARQILVDLSEAANGYGVTLHGLYPEAGGDFDLSVMQASVPQFTGQPMNGRRGIWIDAN
                     ESEGLHLAVDPTGGVVGFGAEQAGDALAGAATDLESFYSLGFPVEGIAAGKPLEIDLK
                     VSTPGVKVRTRRAVALRSPVDRATDRTVSNLFSTVAAPGFPIQARVTSSTPAEKGRVK
                     VAYEVTIPASGLALLPAANARVAKIAAFVALLDKGDVTQAAPVRQEFKTGEDAFADAE
                     GIFTFTGSAVVNPGAVISIGILDEATQESGYARIEVPGAAKK"
     gene            1542..2813
                     /locus_tag="PLB01_12095"
     CDS             1542..2813
                     /locus_tag="PLB01_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78086.1"
                     /translation="MLTFLTLLLGIVWGPREVELSAPAGTAAVELFLDGESVARRTAP
                     PWTLAVDFGSAPAPHHLDAVARDARGVELGRVRQRVNLPKPEAEAVLALLPGKGGKGR
                     VAALRWEGAVGKPRAITLNFDGKPLAALDPEHFDLPAYVPERLHFLQAVIEFEKGARA
                     EAEITFGGRDRDETSRALTAISLRVPRGKLPGVEAMSGWLIADGEPLRVVAAEGGEAS
                     IVFVLDADSPPAFRELADWSILPTRAGMFFSQPEVRMLLAYGTEEFGARTAYDAFPRS
                     FPAYLGKGLLHALAEVFPPGGSLTCPRVADATTAAGLLAASWSHPRAVVVVLTGNLDA
                     SVLSAAQARSYLSDLGVPVVVWTAASAAPEAAARWGDARPVKTLSDVRAAVKELEGAV
                     REQRIVWVEGAHLPQAIFVTAAAPAGVVVAR"
     gene            2824..4101
                     /locus_tag="PLB01_12100"
     CDS             2824..4101
                     /locus_tag="PLB01_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78087.1"
                     /translation="MLTFLTLLLGVVWGQQTLELNAPAGTAAVEIFLDGNSLARRHRP
                     PWTFLVDLGPAPAPHHLDAVAFDARGTEIGRVRQKLNVPRRDAEATLALLPGKGGTGR
                     AARLDWESLVHPAPLRVTVSFDGRPLAFTDPRRIELPPFVPEQVHFLRAVVQLERGFR
                     AEAEITFGGRARDETSRELTAVALRVPGGALPAPEEMAGWLAAGRAPLQVAATEAGEG
                     RVVFVLDAHAPDAFSRLFATLLFSDALSRLRGNPEVRTVFAYKSAVAGSRAGYDVYPR
                     SFPLHMQDGLLRALAEQEPPSEAFEKCPRTADAATAAGLTAAAWSHPRAVVVVLSGDP
                     DASILPAPYARSFLRDLGVPVFVWVVAPGSRDAAAVWGGGRTLRTRAELRAAVKELET
                     ALAEQRIVWVEGTHLPQSIAATSAAPKGVTVAR"
     gene            complement(4102..4620)
                     /locus_tag="PLB01_12105"
     CDS             complement(4102..4620)
                     /locus_tag="PLB01_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQQ78088.1"
                     /translation="MKRIFCLMSILALSACTSAPPPAIRAGDPPAEEAAVLALLDRYM
                     HAISSQDVAAMAAMQTPDGMTYRARAEGGGWAMTSRSNASWVDPSKVDARTHRERYWS
                     PTVHVRGGIAAVWAPYEFWIDGKTSHCGIDVFDFVKVDGAWRVANAMWTVEPNACSEL
                     RPADASTIRPPG"
     gene            complement(4642..4716)
                     /locus_tag="PLB01_12110"
     tRNA            complement(4642..4716)
                     /locus_tag="PLB01_12110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4682..4684),aa:Val,seq:tac)
     gene            4778..6253
                     /locus_tag="PLB01_12115"
     CDS             4778..6253
                     /locus_tag="PLB01_12115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ78089.1"
                     /translation="MERYNLVVIGGGSGGLVVAAGGAGLGARVALVEKHVLPYEPAGR
                     APVQGMGGDCLQYGCVPSKALLAAAKAVHAARGAGRFGIRGAGDPGVQDLGPVMKWVR
                     SVQAEIAPNDCVERFGSLGVDVLLGAGRLKSAHEVEVNGTTVWGRNVVVATGSRARIP
                     DAPGLAEAGFLTNESVFDLRALPKRLLVIGGGPIGVELGQAFQRLGSRVTIVSSSAHV
                     CPKEDADVAAVLAAGLREEGVVIHDEASASSVTVRGGAKVVTVRKKDGTTFDVETDAI
                     LVAAGRRPNVGGLGLEAAGVAFSDKGIVTDAKCRTNVRSVWAIGDVAGPYLFTHWAGY
                     QAGIVLRNTLSPVALAKCDVANTPWVTYSDPEVARVGLSEEDAKAQGIPHRVFRAGFA
                     HNDRAICDGTGGANFMKAVVDPKGRILGAAIVHPHAGDLLGEVVLAKKNGLPLSALGS
                     VIHAYPSLSEIHGAVARDALKTSLTPGRKALLVKLAAFLRR"
     gene            6258..6890
                     /locus_tag="PLB01_12120"
     CDS             6258..6890
                     /locus_tag="PLB01_12120"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HQQ78090.1"
                     /translation="MQELLRSFQGWVVGLGPLGWVLYALVYAFCCVLFVPASILTLGA
                     GALYGLGTGTAVVLAGATLGATLSFLLAKTVLRKKIERMTAGNAKFAALDKAIAREGA
                     KIVFLVRLSPVFPFTYINYAFGLTGVKTWPYVLASFVGMIPGTFAYVYLGAAAATAAS
                     GGTDATRKALQIGGAVAAVAVSIFVARVAAKAIKGAGVGEAATPPAPDAE"
     gene            complement(6900..7490)
                     /locus_tag="PLB01_12125"
     CDS             complement(6900..7490)
                     /locus_tag="PLB01_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQQ78091.1"
                     /translation="MKKTILAVLILGTALTAAASDQSKKPATGPAPAGQTAAGAAAAQ
                     LPKPDFRAFADGQYIFVMNGGDADWTGPLDVKATCKKEGTKQDPTICGPNFPNGSFFY
                     HIAGFQAGHGPSVAPIKGSGNAQQVSGYGWRALRMDLPPGSYEITVTVDPNGKVAEKD
                     ETNNQSVQNVSIAGSASPGAVQVNPGAIQLAPTKKP"
     gene            complement(7608..8258)
                     /locus_tag="PLB01_12130"
     CDS             complement(7608..8258)
                     /locus_tag="PLB01_12130"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQQ78092.1"
                     /translation="MLIRLVRHGESEGNFAGSLQGSRFDTPLSARGRRQAEALAIRLA
                     EEPIDAAWASPMVRAKETASIVAAPHGLPLSIDADLKEFDWGVWSGRPFDATLEMEVS
                     AVRARWRAGETDLAPSGGESPAMAATRAERFLGRLKAAGTRAPLVVAHGRFNRILMAL
                     LLGRPLARMDEIRQRNGSLSVFEWDGAAPATPLLLDDVGHIPGSIRTVSILSDSVK"
     gene            8311..9051
                     /locus_tag="PLB01_12135"
     CDS             8311..9051
                     /locus_tag="PLB01_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78093.1"
                     /translation="MARSARDFPLVSAALDDPAIRALFTERFLVATEIYDALVDAACW
                     KLLGDVGARPAPGEERDEESLLAQYPERARPVLRYTIEKLTDAGFLLRRSGGASAAGV
                     ASTPPDATAPLHLLRESESEFFISQSGREPGGVGALAAELAAVEPDAAVGAEIVSLLA
                     DEGAAFLRGEKTGEEILFSPVRLPLWIRYFSNANVLYAINNTLGAETLTRVLPASGAE
                     VLEIGGGCGSAAEAALSRLGTRASRCRR"
BASE COUNT         1224 a         3358 c         3212 g         1375 t
ORIGIN      
        1 gccgcccccg ccgcgccggc cgccgcgccc gcggagcgcc cgaagcgccg cgtcgtcctc
       61 ttcgtcgacc gcctgacgct cggcgaccag cgccgcagcg cgcgtttctt cggctcgctg
      121 aaggacttcg tcgtccgcac gatccagccc ggtgacgagg cggccgtcct cacgttcgac
      181 gagtcgcttg cgacgcgcgt cccgttcacg ggcgacacct tcgccgtcct caaggcgctc
      241 gacgccctcg cgaaggagag cgcgcgccct ccggccgccc tcaccggcgt cgagacgagc
      301 gagaagctcg tcgcggagat cgcgcaggcc gagcgcgaga tggcgcagcg cgaatccgga
      361 cgaggcggca tcgccacggc gacgattccg acggaagcgg tcgcgccgca ggcggcgccg
      421 atcagcggga cggtcctcgc cgagatgcgc ggtctcgcgg cgcaggagtg gggccggacg
      481 aagaacaagg ccgccgcgct caaagccgtc ctctcggcgc tcggagggct cgacggccgg
      541 cgcgtcctcg tcgtggcgtc gcaccggctg tctcggtggt ccggcatgga gtacttcctc
      601 acgaaacggc tggacgtcga cgtacaggtc ccgccggagg cccgcgagtt cgacgcgcgc
      661 cagatcctcg tcgacctctc cgaggccgcg aacggatacg gcgtcacgct ccacggcctc
      721 taccccgaag cgggcggaga cttcgacctc tccgtcatgc aggcttcggt accgcagttc
      781 acggggcagc cgatgaacgg gcggcgcggg atctggatcg acgcgaatga gagcgagggc
      841 ctccacctcg ccgtggatcc cacgggcgga gtcgtcggct tcggcgccga gcaggccggc
      901 gacgccctcg cgggcgcggc caccgacctc gagtcgttct actcgctggg attcccggtc
      961 gagggcatcg cggccggcaa gcctctcgag atcgacctca aggtgtcgac gccgggcgtg
     1021 aaagtgcgga cgcgccgcgc cgtcgccctc cggagccccg tggatcgcgc caccgaccgc
     1081 accgtgtcga acctcttcag cacggtcgcg gcgccggggt tcccgatcca ggcgcgcgtg
     1141 acctcgtcca cgcccgccga gaagggccgc gtgaaggtcg cgtacgaggt gacgatcccg
     1201 gcctcgggcc tcgccctcct ccccgcggcg aacgcacgcg tcgcgaagat cgcggccttc
     1261 gtcgcgctcc tcgacaaggg cgacgtcacc caggccgcgc cggtgcgcca ggagttcaag
     1321 acgggcgagg acgccttcgc ggacgccgag gggatcttca cgttcacggg ctcggccgtc
     1381 gtcaacccgg gcgccgtgat ctcgatcggc atcctcgacg aggcgacgca ggaatccggt
     1441 tacgcccgca tcgaggtgcc gggggcggcg aagaaatagc gcgcgcctga tacagccgct
     1501 cccttgcaaa cgcgcctcgg caggcgccat cgtcagtctg gatgctgacg ttcctgacgc
     1561 tcctcctcgg aatcgtctgg ggaccgcggg aagtcgagct cagcgcgccg gccggaacgg
     1621 cggcggtcga gctctttctc gacggggaga gcgtcgcgcg gcgcaccgcg ccgccctgga
     1681 ctctcgccgt ggatttcggc tccgcacccg ctccgcacca tctcgacgcc gtcgcccgcg
     1741 acgcgagggg cgtcgagctc ggccgcgtgc gccagcgcgt aaacctcccg aagcccgagg
     1801 ccgaggcggt tctcgccctc cttcccggca agggcggaaa ggggcgcgtc gccgcccttc
     1861 gctgggaggg tgccgtgggg aaacctcgcg cgatcaccct gaacttcgac ggcaagcctc
     1921 tggccgcact cgaccccgag cacttcgact tgcccgcgta cgtccccgaa cgtctccatt
     1981 tcctgcaggc cgtcatcgaa ttcgagaagg gcgcgcgcgc cgaggctgag atcacgttcg
     2041 ggggacgcga tcgcgacgag acctcgcgcg cgctcacggc aatctccctg cgcgtcccgc
     2101 ggggaaagct cccgggggtg gaagccatgt ccgggtggct catcgcggac ggcgagcctc
     2161 tgcgcgtcgt cgcggcggag ggtggcgagg cgtcgatcgt cttcgtcctc gacgcggact
     2221 ctcctcccgc attccgagag ctcgcggact ggtcgatcct ccccaccagg gcgggcatgt
     2281 tcttcagtca gcccgaagtg cgcatgctcc tcgcctacgg cacggaggag ttcggtgcac
     2341 ggaccgcgta cgacgccttc ccccgctcgt tccccgcgta cctgggaaag ggcctcctcc
     2401 acgccctcgc ggaggtcttt ccgccgggag gcagtctcac ctgtccgcgc gtcgcggatg
     2461 ccacgacggc ggcgggtctc ctcgcggcat cctggtcgca cccccgggcc gtcgtcgtcg
     2521 tcctgaccgg gaatctcgac gcgagcgtgc tctccgccgc ccaggcccgc tcgtatcttt
     2581 cggacctcgg cgtccccgtc gtcgtatgga ccgccgcgtc cgcggcgccc gaggccgcag
     2641 cccgatgggg cgatgcccgc ccggtgaaga ccctttccga cgtgcgcgct gcggtcaagg
     2701 agctggaggg cgcggtacgg gagcagcgga tcgtctgggt cgagggcgca caccttccgc
     2761 aggcgatttt cgtcaccgcg gccgctcccg cgggcgtggt ggtcgcgagg tgacacgctt
     2821 cccgtgctga cattcctgac cctcctcctc ggcgtggtct gggggcagca gaccctcgag
     2881 ctgaacgctc cggccgggac ggcggccgtg gagatcttcc tcgacggcaa cagcctcgcc
     2941 cggcggcatc ggcccccgtg gacgttcctc gtggatctcg gtcccgcgcc cgcgccccat
     3001 cacctcgacg ccgtcgcgtt cgacgcccgc gggaccgaga tcggccgcgt gcgccagaag
     3061 ctgaacgttc cccggcgcga tgccgaggcg acgctcgcgc tcctgccggg caagggcggg
     3121 accggccgcg ccgcgcgcct cgactgggag tcgctcgtgc acccggcgcc cctccgcgtg
     3181 acggtgagct tcgacggccg gcccctcgcg ttcacggatc cccgccgcat cgagctcccg
     3241 ccgttcgtgc ccgagcaggt gcactttctc cgcgccgtcg tgcagctcga gcgcggcttc
     3301 cgcgcggagg ccgagatcac gttcggcggg cgcgcgcgcg acgagacgtc ccgggagctc
     3361 acggcggtcg ccctccgggt ccccggcggc gcgcttcccg cgccggagga gatggccggc
     3421 tggctcgccg ccggccgagc accgctccag gttgccgcga cggaagccgg tgaaggccgc
     3481 gtcgtgttcg tcctcgacgc acacgctccc gatgcgttct cgcgcctctt cgcgactctc
     3541 ctcttcagcg acgccctctc caggttgaga ggcaacccgg aggtccggac cgtgttcgcg
     3601 tacaagagcg cggtcgccgg ctcgcgcgcc gggtacgacg tctacccacg ctcgttccct
     3661 ctccacatgc aggacggcct cctgcgcgcc ctcgcggaac aggagccgcc ctccgaggct
     3721 ttcgagaagt gcccccgcac ggcggacgcc gcgacggccg ccgggctcac cgccgcggcg
     3781 tggtcgcatc cgagggccgt cgtcgtcgtc ctgtccggcg atcccgacgc gagcatcctc
     3841 ccggccccct atgcacgctc gtttctgcgg gacctgggcg ttccggtctt cgtctgggtc
     3901 gtcgcgcccg gctcgcgcga cgcggccgcg gtctggggcg gcggccgcac cctgaggacg
     3961 cgcgccgagc tccgcgccgc cgtgaaggag ctggagaccg cgctcgccga gcagcggatc
     4021 gtctgggtgg aaggcacgca cctcccccag tcgatcgcgg ccacatccgc cgcgccgaag
     4081 ggcgtcaccg tcgcgaggtg atcaccccgg gggccggatc gtcgaggcgt ccgcgggccg
     4141 caactcggag cacgcgttcg gctcgaccgt ccacatcgcg ttggcgacgc gccaggcgcc
     4201 gtccaccttc acgaagtcga acacgtcgat tccgcagtgg ctcgtcttgc cgtcgatcca
     4261 gaattcgtag ggcgcccaga ccgccgcgat gccgccgcgg acgtggacgg tgggcgacca
     4321 gtaccgctcg cggtgcgtcc gcgcgtcgac cttcgacgga tccacccacg acgcgttcga
     4381 ccgcgacgtc atggcccagc cgccgccctc ggcgcgggcc cggtacgtca tcccgtccgg
     4441 cgtctgcatc gccgccatcg ccgcgacgtc ctgggaggag atcgcgtgca tgtaacggtc
     4501 gagcagcgcg agcaccgcgg cctcctcggc cggcgggtcc ccggcgcgga tagcgggcgg
     4561 aggcgcggag gtacacgcgg agagagccag gatcgacatc aagcagaaga tccgtttcat
     4621 gtgagttcct cgatcgtgtg ctggtgggag gtgccgggat cgaaccggcg acctgctgcg
     4681 tgtaaagcag acgctctacc gctgagctaa cctcccggct ccagtttacg cggggaacaa
     4741 cgcgggcgga gccggtcatt aaccccgggg agacgtgatg gaacgctaca acctcgtcgt
     4801 catcggcggc ggatccggcg gcctcgtcgt cgccgccggc ggtgcgggac tcggggcgcg
     4861 cgtcgcgctc gtcgagaagc acgtcctccc ctacgagccg gcgggccggg cgcccgtcca
     4921 gggcatgggg ggcgactgcc tgcagtacgg ctgcgtcccg tccaaggcgc tcctcgccgc
     4981 cgcgaaggcc gtccacgccg cgcgcggcgc cgggcgtttc ggcatccgcg gcgcgggcga
     5041 tccgggagtc caggacctcg ggcccgtgat gaaatgggtg cgctcggtgc aggccgagat
     5101 cgcgccgaac gactgcgtcg agcgcttcgg gagcctcggc gtcgacgtcc tcctcggcgc
     5161 gggccgcctg aagtcggccc acgaggtcga ggtgaacggg acgacggtgt ggggccggaa
     5221 cgtcgtcgtc gcgacgggct cgcgcgcgcg catcccggac gcgcccggcc tcgccgaggc
     5281 cggcttcctc acgaacgaga gcgtcttcga tctccgcgct ctcccgaaac gcctcctcgt
     5341 gatcggaggc ggcccgatcg gcgtcgagct cggccaggcg ttccagcgcc tcggatcccg
     5401 cgtcacgatc gtgtcgtctt ccgcgcacgt ctgcccgaag gaggacgccg acgtcgcggc
     5461 cgtcctcgcg gccggcctgc gcgaggaggg cgtcgtgatc cacgacgagg cttcggcctc
     5521 gtcggtcacc gtgcgcggcg gcgcgaaggt cgtgaccgtc aggaagaagg acgggacgac
     5581 gttcgacgtg gagacggacg cgatcctcgt cgcggccggc cgccgcccga acgtgggcgg
     5641 cctcggcctc gaggcggcgg gcgtcgcgtt ctcggacaag ggcatcgtga cggatgcgaa
     5701 gtgccggacg aacgtgcggt cggtctgggc gatcggcgac gtcgcgggcc cgtacctctt
     5761 cacgcactgg gcgggctacc aggccgggat cgtcctccga aacacgctct cccctgtcgc
     5821 gctcgcgaag tgcgacgtgg ccaacacgcc ctgggtcacg tactcggacc ccgaggtcgc
     5881 ccgcgtcggg ctctcggagg aggacgcgaa agcgcagggg attccgcacc gcgtcttccg
     5941 cgcgggattc gcccacaacg accgcgcgat ctgcgacggc accggcggcg cgaacttcat
     6001 gaaggccgtc gtggacccga agggccgcat cctcggcgct gcgatcgtcc acccgcacgc
     6061 gggcgacctc ctcggcgagg tcgtcctcgc caagaaaaac gggctccccc tctcggcgct
     6121 cgggtccgtc atccacgcgt acccgtcgct ctccgagatc cacggcgccg tcgcgcggga
     6181 cgcgctgaag acgtcgctca cgcccggccg caaggcgctc ctcgtgaagc tcgcggcgtt
     6241 cctgaggcgg tagccgcgtg caggagctgc tccggagctt ccagggctgg gtcgtgggcc
     6301 tcgggccact cggctgggtg ctctacgcgc tcgtctacgc cttctgctgc gtgctcttcg
     6361 tgccggcgtc gatcctgacg ctcggagcgg gcgcgctcta cggcctcggg accggcacgg
     6421 cggtcgttct cgcgggcgcg acgctgggtg cgacgctctc gttccttctc gcgaagacgg
     6481 ttctccggaa gaagatcgaa aggatgactg cggggaacgc gaagttcgcg gcgctggaca
     6541 aggcgatcgc cagggagggc gcgaagatcg tcttcctcgt gcggctctcg cccgtcttcc
     6601 cgttcacgta catcaactac gcgttcgggc tcacgggcgt gaagacctgg ccctacgtcc
     6661 tcgcgtcctt cgtcgggatg atcccgggca ccttcgccta cgtgtacctc ggcgccgcag
     6721 cggcgacggc agcatccggg gggacggacg cgacgaggaa ggcgctccag atcggcggcg
     6781 ccgtcgccgc ggtcgccgtg tcgatcttcg tcgcacgcgt cgccgcgaag gcgatcaagg
     6841 gcgcgggcgt cggcgaggcc gcgaccccgc cggcgcccga cgcggagtga ttcggaccct
     6901 cagggcttct tcgtcggcgc gagctggatc gcgcccggat tcacctggac cgcaccgggc
     6961 gaagccgagc cggcgatcga gacgttctgc acggactggt tgttcgtctc gtccttctcg
     7021 gccaccttgc cgttcggatc caccgtgacc gtgatctcgt acgaacccgg gggcaggtcc
     7081 atccggagag cgcgccagcc gtagccggag acctgctgcg cgttgccgga gcccttgatc
     7141 ggcgcgacgc tcgggccgtg gccggcctgg aagccggcga tgtggtagaa gaaactcccg
     7201 ttcgggaagt tcgggccgca gatcgtgggg tcctgtttcg tcccctcctt cttgcacgtc
     7261 gccttcacgt cgagcggacc cgtccagtcg gcgtcccctc cgttcatcac gaagatgtac
     7321 tggccgtcgg cgaaggccct gaagtcgggc ttcggaagct gcgcggcggc ggcgccggcc
     7381 gccgtctggc cggcgggcgc gggtccggtc gccggcttct tgctctggtc cgatgcggcg
     7441 gccgtgagcg cggttccaag gatcaggacg gcgaggatgg tcttcttcac gggctctcct
     7501 tctttttgcg tctcggtctc acggggaatc tacgggtttt gcggacgttg gcaaggacgc
     7561 cgcgtggtct cgccacgcca gaagcctgtt ccgaggcgtc agccggccta cttgacgctg
     7621 tccgacagga tcgagacggt ccggatcgaa ccgggaatgt ggccgacgtc gtccaggaga
     7681 agcggcgtcg ccggggcggc tccgtcccac tcgaagaccg agagggagcc gttccgctgc
     7741 cggatctcgt ccatccgcgc gagcggccgg ccgagaagca gcgccatgag gatgcggttg
     7801 aacctcccgt gggcgacgac gagcggcgcc cgcgtgccgg ccgccttcag gcggccgaga
     7861 aaccgttcgg ccctcgtcgc ggccatcgcc ggagactcgc cgcccgacgg cgcgaggtcc
     7921 gtctcccccg cccgccagcg cgcgcgcacg gccgaaacct ccatctcgag agtggcgtcg
     7981 aacggccgcc cggaccagac cccccagtcg aactccttga ggtccgcgtc gatcgagagc
     8041 ggaaggccgt gcggcgccgc gacgatcgac gccgtctcct tcgcccgcac catcgggctc
     8101 gcccacgcgg cgtcgatcgg ctcctccgcg agccggatcg cgagcgcctc ggcctggcgc
     8161 cgcccgcgcg ccgagagcgg cgtgtcgaaa cgcgagcctt gcagggagcc tgcgaagttg
     8221 ccttcgctct cgccgtgccg cacgagccgg atcagcatgt tggcggcgga ttctatgccg
     8281 ggagacgccc tcgcctatca tccgctccgg atggcccgct ccgcgcgcga cttcccgctc
     8341 gtctcggccg ccctcgacga cccggcgatc cgcgcgctct tcacggagcg cttcctcgtc
     8401 gcgaccgaga tctacgacgc cctcgtggac gcggcgtgct ggaagctgct cggcgacgtc
     8461 ggcgcgcggc ccgcgcccgg ggaagagaga gacgaagaga gtcttcttgc tcaatacccc
     8521 gagcgagccc ggccggttct ccgatacacg atcgagaagc tgacggacgc gggatttctt
     8581 cttagaaggt cgggtggcgc ctccgcagcg ggagttgcct cgacgccgcc cgacgccact
     8641 gcccctcttc accttctaag agaatccgaa tccgaattct tcatctctca atccgggagg
     8701 gagcccggcg gggtcggggc cctcgccgcc gagctcgcgg ccgtcgagcc ggacgccgcc
     8761 gtcggcgccg agatcgtctc gctcctcgcc gacgagggcg ccgcgttcct ccgcggcgag
     8821 aagacgggcg aggagatcct gttctccccc gtgcggctgc cgctctggat ccgctacttc
     8881 tcgaacgcga acgtcctgta cgccatcaac aacacgctcg gcgcggaaac gctgacgcgc
     8941 gtgctgcccg catcgggcgc cgaggtcctg gagatcggcg gcggctgcgg aagcgccgcg
     9001 gaggccgcgc tctcccggct cggaacgcgc gcgtcgcgct gccgtcgctg accgagacga
     9061 cgacgccgcc gtagctgccg gcggcgctgt agctgggggt ccactggatc aggccgctgg
     9121 cgtcgatcgt cgcgccgggc ggcaggcgtt cggcctggta gctcagcgc
//
LOCUS       DAOMFY010000147         9058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2339_length_9058_cov_8.142286, whole genome shotgun sequence.
ACCESSION   DAOMFY010000147 DAOMFY010000000
VERSION     DAOMFY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2339_length_9058_cov_8.142286"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLB01_12140"
     CDS             <1..190
                     /locus_tag="PLB01_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78094.1"
                     /translation="QRVFEELSVSTPQDPGAWLMLGRTRRCTGDASGARAALDRAARL
                     GGNPAAIAREIEALGGSR"
     gene            190..2154
                     /locus_tag="PLB01_12145"
     CDS             190..2154
                     /locus_tag="PLB01_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQQ78095.1"
                     /translation="MPDARRLVALLLAAALPALSAPPAKPAAPAPKRARVILVGWDGA
                     DWKYLDPLLKAGALPNLAALVAKGRTWNLDTYQPMASPLVWTTVATGRTPVDHGVTDF
                     QELDPKTGVRLPISGRSRRVPAIWNLASAKGLTVGVVGWWATWPAEKVNGFLVSDRAA
                     PVLFDPESLARSPAVAWPEGLGDGVRMIGRREGSPPYEDVAKALRVSRAEFDAAVAAK
                     KDLADPITGFRKILGVTRTIAKIGLELYDRSSLDLFMIYFQGTDEIGHVLGRYEAPKL
                     PAVSEDDHRKFRDGIAAIYVEADRVLGELAARAQKDGATLILASDHGFRHGADRPAFV
                     SGTQFDTAFLWHEPPGILVAAGPGVIPSPARGKASVFDLAPTLCRLLGLPADPAFEGK
                     LVPGFTGRPAVPAVSWAKAAPVERLVPATNAADEKKAADEFTKQLISLGYLTGAEASA
                     VDARPASRAGTETAGSFQNVGTFLRDRGKPAEAVGWYRKALEVNPKSATAWVNLSTGL
                     HMTGKYDESDDALLAALANGYHDPEGTVYRRVKLYTEAKGRDLRPQLVSFLKKVVAAY
                     PKEDRYRASLGKAYFETGDCASARPLFEELSGRPAPEAESLNLLALTSWCLGDLARAK
                     AAFERSLAINPNQPVVREGLATVEKGRPMR"
     gene            2151..3089
                     /locus_tag="PLB01_12150"
     CDS             2151..3089
                     /locus_tag="PLB01_12150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ78096.1"
                     /translation="MSVPRKVSVVVPVYNEEANLPLLLPRLLPVLAGLGRPFEVVFAD
                     DGSRDRSLEILKAAAAANPGTVKVVELVRNAGQHMAILAAFTATDGELVITLDADLQN
                     PPEEIPKVVAAMDAGHDVVGTVRQDRQDAVFRKVASRLVNRTTALITGMRLNDYGCML
                     RGYHRDVVDVMSASDEAATFIPALAQQYARRPVEIPVAHAERTAGESKYSLYKLVRLN
                     FDLMTGFSVVPLQVFGLLGFVTAVSGVAFGVFLLVRRLVVGAEVDGVFTLFALAFVVM
                     GVLMAGIGIVGEYVGRIYQQVRGRPRFLIRKVWGAN"
     gene            3089..4033
                     /locus_tag="PLB01_12155"
     CDS             3089..4033
                     /locus_tag="PLB01_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HQQ78097.1"
                     /translation="MGGLVVCAYSDVGYKCLEWLFDQGESVRLVFTHPDTPGEERWFG
                     SVADLARKHGIEPRIVNDLNDPADEERIRAVQPDFLFSFYFKKMIPERVLSVPKHGSL
                     NMHGSLLPAFRGRSPVNWAILKGATETGASLHYMTGKPDAGDLVDCERVKIGPDDDIL
                     GVSRRVGDAAVTVLARAMPKLKAGTAPRVPLDLAKGSYFGGRKPEDGEIDWTKSAKEV
                     HDLVRAVTKPWPGAFTDVFGKTVTIWKTRPAPYAGHDVFPGKVELTESSVVVYAGDDR
                     TLEILSARPEGGMDLDAGAFRDWLVRPSGIFPRVKEDT"
     gene            4030..5061
                     /locus_tag="PLB01_12160"
     CDS             4030..5061
                     /locus_tag="PLB01_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HQQ78098.1"
                     /translation="MRVLILGVNGFIGNALTRRILTTTPWEVFGLDVGSERIEEFLGE
                     KRFRFLEGDISINREWIEYHVKKCDVVLPLVAIATPATYVKNPLSVFELDFEENLRIV
                     KMAGKYGKRVVFPSTSEVYGMCPDKEFDEDRSPLVYGPIAKPRWIYSASKQLLDRVIA
                     AMGTAQGLQYTLFRPFNWYGPHLDNVDAPKEGSSRVLTQFLHNILYGVPIKLVDGGAQ
                     RRCFTYIEDGIDGLMTILENPKGKADGQIFNLGNPSADLSIEQLAHALIDAVATYPKF
                     AAAAKKTRIVKVSSKEYYGEGYQDITTRVPSVAKAKKLLGWTPKTDFPTGLKKTLDFY
                     LKGRKPPEV"
     gene            5058..6038
                     /locus_tag="PLB01_12165"
     CDS             5058..6038
                     /locus_tag="PLB01_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQQ78099.1"
                     /translation="MSRLALKVDVDTYRGTLEGVPRLLDLFAREGVKATFFFSLGPDT
                     SGKAIKRIFRKGFVKKVLSASPAASYGVKTMLYGTLLPAPDIGGRAETVARMREAAKA
                     GHSVGIHAWDHIDWHDHLPGMSREEIDAVVAREHARFLEIFGAPAAFQAAPGWTATPL
                     SVEVQEAHGILASSDTRGGEPFFALRADGTPSRVLEIPSTLPTLDELLALPLRGAGTQ
                     AERACEFLRVNVKEGMHVHSIHTEIEGATSFVAPFASLVAAWKRDGVTFLTFEEICRP
                     LLSMRALAGSAREQREIPVKKLVWTTLPGRATPVATGVATGVAPGASSAA"
     gene            6035..6493
                     /locus_tag="PLB01_12170"
     CDS             6035..6493
                     /locus_tag="PLB01_12170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQQ78100.1"
                     /translation="MKSLQESYGPNTVCFGCGPSNEKGLKIRSFAQGDEVVATWQAEP
                     HHLAFEGVLNGGIIGALLDCHSNWTAAWHLMQKARDGVLPSTVTADFHVVLKRPTPIA
                     EPLHLKAHVVESTADRAVVEATIEAGGKVTATCRGTFVAVKEGHPAFHRW"
     gene            6490..7863
                     /locus_tag="PLB01_12175"
     CDS             6490..7863
                     /locus_tag="PLB01_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78101.1"
                     /translation="MTGAAAPAAPAAPPSRTFVALFFLLVLAALLFSDKGQIAGDGEV
                     RWGALVALMDEGRHTPDRYTLEMPIAAAPLWAAGAAAARVEGAPGAARLDTIRRVVQR
                     FNKLVAFAIAVWLFRRLRGLGLDEFAAAGSVLILLFASLLVPHSKDFYSESLWTLLAC
                     VALGQLADGPARTRAGDAALAVAAALAIPLNPLLCPVIFVTSAMLFFLEGEEGRSAAR
                     RGAVLASAGAALGLGLALSENLLRRGALLDFGYAGEGFTAPFFEGLFGQLFSPARGAL
                     FYLPLFFVGILLLGRRARPDVRRFTLAGATFGVLLVLAYAKWHAWHGMTYWGPRFLLP
                     LSIFGAAFVALAWRESPRPSWRTALVLLVAISYAAYKVGVGVGVVPLRACLLPDPARL
                     SCYWDWARLPLASWLSPGDLASMLSHRSTAVEAGTLVLFAVLVALTGRGRSRRAIFPQ
                     GPAPRSG"
     gene            complement(7799..>9058)
                     /locus_tag="PLB01_12180"
     CDS             complement(7799..>9058)
                     /locus_tag="PLB01_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78102.1"
                     /translation="RAAYEALPALHVLRYPVKALLPFTMALAALAALGVDRLLVEEAL
                     PRFRRRASRALLGLAVLFGLLAIVTRLSPRILFAVLVRLWDPAWASPPGIVLAPVLER
                     IPSEAALAGAGLAVLGLLLARGARDARGQGLLLAATAVSLLAYTPTAIPRVPSEIFRE
                     PSPLVARAAALGGRVYERAAKDFDAVRRGAQGRVVTGDILDLSLAQARQGWALSGAPH
                     GLAYAWDPDPDGSYTILNRYASDVVKQAPWGRRLKWLRAAGVRAVIAADVPPETPGLT
                     PVYVEGSAGVPVTLWRVTDPLPGLRRAGRIVVAGSINATVSAFEDAGFDPATDVVVHA
                     RDAASVSSSVRDAAAEARFVEDGADRLVVETSGARAGILRVDRSFTPRATASVDGKSA
                     TVYAADLNLIGVPVPAGKSRVVIDLAP"
BASE COUNT         1319 a         3216 c         3185 g         1338 t
ORIGIN      
        1 gcagcgcgtg ttcgaggagc tctccgtctc gacgccgcag gatcccggcg cctggctcat
       61 gctcggccgt acgcgccggt gcacgggcga cgcctccggc gcgcgcgccg ctctcgaccg
      121 cgccgcgcgc ctcggcggca accccgccgc gatcgcgcgc gaaatcgaag ccctcggagg
      181 ttcccgctga tgcccgacgc ccgccgcctc gtggccctcc tgctcgccgc cgcgcttccg
      241 gcgctctcgg cgccgcccgc gaagcccgcg gctcccgcgc cgaagagggc ccgcgtcatc
      301 ctcgtcggct gggacggcgc ggactggaag tacctcgacc cgctgctcaa ggcgggcgcg
      361 cttccgaatc tcgcggccct cgtcgcgaag gggcggacgt ggaacctcga cacgtaccag
      421 cccatggcgt cgccgctcgt ctggacgacg gtcgccacgg gccgcacccc cgtggaccac
      481 ggcgtgacgg acttccagga gctggacccg aagacgggcg tgcgcctccc gatctcgggc
      541 cgctcgcgcc gcgttcccgc gatctggaac ctcgcctcgg cgaagggcct cacggtgggc
      601 gtcgtgggct ggtgggcgac gtggcccgcg gaaaaagtga acggcttcct cgtctccgac
      661 cgcgccgcgc cggtcctctt cgaccccgag tcgctcgcgc gctcgcccgc ggtcgcgtgg
      721 ccggaggggc tcggcgacgg cgtgcggatg atcggccgcc gcgagggcag cccgccgtac
      781 gaggacgtcg cgaaggcgct ccgcgtctcg cgcgccgagt tcgacgcggc ggtcgcggcg
      841 aagaaggacc tcgcggaccc gatcaccggc tttaggaaga tcctcggcgt gacgcggacg
      901 atcgcgaaga tcggcctcga gctctacgac cgttcgagcc tcgacctctt catgatctat
      961 ttccagggca cggacgagat cggccacgtc ctcggacgct acgaagcccc gaagctgccg
     1021 gcggtttccg aggacgacca ccggaagttc cgcgacggca tcgcggcgat ctacgtggag
     1081 gcggaccggg tcctcggcga gctcgcggcg cgcgcgcaga aggacggcgc gacgctgatc
     1141 ctcgcgtccg accacggctt ccggcacggc gcggatcgcc ccgcgttcgt gtccgggacg
     1201 cagttcgaca cggcgttcct ctggcacgag ccgcccggca tcctcgtggc ggccgggccg
     1261 ggcgtgatcc cgtcgcccgc ccgcggcaag gcgagcgtct tcgatctcgc cccgactctc
     1321 tgccgtctcc tcggcctgcc ggcggacccg gcgttcgagg gaaagctcgt tcccggcttc
     1381 acgggccggc cggcggtccc ggccgtgtcc tgggcgaagg ccgcacccgt cgaacgcctc
     1441 gtgcccgcga cgaacgcggc ggacgagaaa aaggccgccg acgagttcac gaagcagctc
     1501 atctcgctcg ggtacctcac gggcgccgag gcgtcggcgg tggacgcgcg gcccgccagc
     1561 cgcgcgggca ccgagaccgc cggctcgttc cagaacgtgg ggacgttcct gcgcgaccgc
     1621 ggaaagcccg ccgaggccgt cggctggtac cgcaaggcgc tcgaagtgaa cccgaaatcg
     1681 gccacggcct gggtgaacct ctcgaccgga ctccacatga cggggaagta cgacgagtcg
     1741 gacgacgcgc tccttgccgc gctcgcgaac gggtatcacg acccggaagg aaccgtgtac
     1801 cggcgcgtga agctctacac ggaggcgaag gggagggacc tgcgcccgca gctcgtgtct
     1861 ttcctgaaga aagtcgtcgc ggcgtacccg aaggaggacc gctaccgggc ctcgctcgga
     1921 aaggcgtact tcgaaaccgg cgactgcgcg agcgcacggc cgctcttcga ggagctctcg
     1981 ggccgcccgg cgccggaggc ggagagcctg aatctcctcg ccctcacgag ctggtgcctc
     2041 ggcgacctcg cgcgcgcgaa ggccgccttc gagcgctcgc tcgcgatcaa tccgaaccag
     2101 ccggtcgtgc gcgagggcct cgcgaccgtc gagaagggga gaccgatgcg atgagcgtcc
     2161 cgcgaaaggt ctcggtcgtc gtgcccgtct acaacgagga ggcgaacctc ccgctgctcc
     2221 tcccgcgcct cctgccggtg ctcgcggggc ttggccggcc gttcgaggtc gtgttcgcgg
     2281 acgacggttc gcgcgaccgc tcgctcgaga tcctcaaggc cgccgccgcc gcgaacccgg
     2341 ggaccgtcaa ggtcgtcgag ctcgtccgga acgcgggcca gcacatggcg atcctcgcgg
     2401 ccttcacggc gacggacgga gagctcgtca tcacgctcga cgcggacctc cagaacccgc
     2461 ccgaggagat ccccaaggtc gtcgcggcca tggacgccgg gcacgacgtc gtcggcacgg
     2521 tccgacagga ccgccaggac gccgtgttcc gcaaagtcgc gtcccggctg gtcaacagga
     2581 cgacggctct catcacgggg atgaggctga acgactacgg ctgcatgctc cgcggctacc
     2641 accgggacgt cgtggacgtc atgtccgcct cggacgaggc ggcgacgttc atcccggccc
     2701 tcgcccagca gtacgcgcgg cggccggtgg aaatccccgt cgcccacgcc gagcgcacgg
     2761 cgggcgagtc gaagtattct ctctacaagc tcgtgcgcct gaacttcgac ctcatgaccg
     2821 gattctccgt cgtcccgctg caggtgttcg ggttgctcgg gttcgtgacg gcggtctccg
     2881 gggtcgcgtt cggcgtcttc ctcctcgtcc gccggctcgt ggtcggcgcc gaggtcgacg
     2941 gcgtgttcac gctgttcgcg ctcgccttcg tcgtcatggg agtcctgatg gccggaatcg
     3001 ggatcgtggg cgagtacgtg ggccgcatct accagcaggt gcgcggccgg ccgcgcttcc
     3061 tcatccggaa ggtgtggggg gcgaactgat gggcggcctc gtcgtctgcg cgtactcgga
     3121 cgtcggctac aagtgtctcg agtggctctt cgaccagggc gagagcgtgc gcctcgtgtt
     3181 cacgcacccc gacacgcccg gcgaggagcg ctggttcggc tccgtcgcgg acctcgcgcg
     3241 caagcacggc atcgagcccc gaatcgtgaa cgacctgaac gacccggccg acgaggagcg
     3301 catccgcgcc gtgcagccgg acttcctgtt ctcgttctac ttcaagaaga tgatcccgga
     3361 gcgcgtcctg tcggtgccga agcacgggtc cctgaacatg cacggctccc tcctcccggc
     3421 attccgcggc cgctcgcccg tgaactgggc gatcctgaag ggggcgacgg agacgggcgc
     3481 ctcgctccac tacatgacgg gaaaaccgga cgcgggcgac ctcgtggact gcgagcgcgt
     3541 gaagatcggg cccgacgacg acatcctcgg cgtctcgcgc cgcgtgggcg acgcggcggt
     3601 cacggtgctc gcgcgcgcga tgccgaagct caaggccggc acggcgccgc gggtgcctct
     3661 cgacctcgcg aaggggagct acttcggcgg gcgcaagccc gaggacggcg agatcgactg
     3721 gacgaaaagc gcgaaggagg tccacgacct cgtccgcgcc gtgacgaagc cgtggccggg
     3781 cgcgttcacg gacgtcttcg gaaagaccgt cacgatctgg aagacgcgcc ccgccccgta
     3841 cgcggggcac gacgtcttcc cggggaaggt cgagctgacg gagagctccg tcgtcgtcta
     3901 cgcgggcgac gaccggacgc tcgagatcct ctccgcgcgt ccggagggcg ggatggatct
     3961 cgacgcgggc gccttccgcg actggctcgt gcgcccgtcg ggcatctttc cgcgcgtaaa
     4021 ggaagacaca tgagagtcct gatcctgggt gtgaacggtt tcatcggaaa cgccctcacg
     4081 cggcgaatcc tcacgacgac gccctgggag gtgttcggcc tcgacgtcgg gagcgagcgg
     4141 atcgaggagt tcctgggcga gaagcgcttc cgtttcctcg agggcgacat ctcgatcaac
     4201 cgcgagtgga tcgagtacca cgtcaagaag tgcgacgtcg tcctgccgct cgtcgcgatc
     4261 gcgacgcccg cgacgtacgt gaagaacccg ctctccgtct tcgagctgga cttcgaggag
     4321 aacctgcgga tcgtcaagat ggcgggcaag tacggcaagc gcgtcgtctt cccgtccacg
     4381 tccgaggtgt acgggatgtg cccggacaag gagttcgacg aggaccgctc gccgctcgtc
     4441 tacgggccga tcgcgaagcc gcgctggatc tactcggcgt ccaagcagct cctcgaccgg
     4501 gtcatcgcgg cgatggggac cgcgcagggc ctccagtaca cgctcttccg gccgttcaac
     4561 tggtatgggc cgcacctcga caacgtggac gccccgaagg agggctcgag ccgcgtcctg
     4621 acgcagttcc tgcacaacat cctctacggc gtcccgatca agctcgtgga cggcggcgcg
     4681 cagcgccggt gcttcacgta catcgaggac gggatcgacg gcctcatgac gatcctcgag
     4741 aacccgaagg ggaaggccga cggccagatc ttcaacctcg gaaacccgtc cgccgacctc
     4801 tcgatcgagc agctcgcaca cgcgctcatc gacgcggtcg cgacgtaccc gaagttcgcg
     4861 gccgccgcga agaagacgcg gatcgtgaag gtctcctcga aggagtacta cggcgagggc
     4921 taccaggaca tcacgacgcg cgtcccgtcg gtcgcgaagg cgaagaagct gctcgggtgg
     4981 acgccgaaga cggacttccc gacgggcctg aagaagacgc tcgacttcta cctcaagggc
     5041 cggaagcccc cggaagtttg agcaggctcg cactcaaggt cgacgtcgac acctaccgcg
     5101 gcacgctcga gggcgtgccg cgcctcctcg acctcttcgc acgggagggc gtgaaggcga
     5161 cgttcttctt ctcgctcggc ccggacacga gcggcaaggc catcaagcgc atcttccgca
     5221 agggcttcgt gaagaaggtc ctctccgcga gccccgcggc ttcgtacggc gtcaagacga
     5281 tgctctacgg cacgctcctg cccgcgccgg acatcggcgg gcgggcggag acggtcgcgc
     5341 gcatgcgcga ggccgcgaag gcgggccact ccgtcgggat ccacgcgtgg gaccacatcg
     5401 actggcacga ccatctcccc gggatgtcgc gcgaggagat cgacgcggtc gtcgcgaggg
     5461 agcacgcgcg cttcctcgag atcttcgggg cgccggccgc gttccaggca gcgccgggct
     5521 ggacggcgac gccgctctcc gtcgaggtgc aggaggcgca cggcatcctc gcgtcgtcgg
     5581 acacgcgggg cggcgagccc ttcttcgcgc tgcgggcgga cggcacgccg agccgggtgc
     5641 tcgagattcc gtccacgttg cccacgctcg acgagctgct cgcccttccg cttcgaggcg
     5701 caggaactca ggccgagagg gcctgcgaat ttcttcgagt gaatgtgaaa gagggaatgc
     5761 acgttcactc gattcacacg gagatcgagg gtgcgacgtc gttcgtcgcg ccgttcgcct
     5821 cgctggtcgc cgcgtggaag cgcgacgggg tgacctttct taccttcgaa gaaatttgca
     5881 gaccgttgct ctccatgagg gcgcttgcag gatccgcacg ggagcaaagg gagatcccgg
     5941 tcaagaagct cgtgtggacg acactcccgg ggagggcgac gccggtcgcc accggggtgg
     6001 cgaccggcgt cgcacccggg gcatcatccg ccgcatgaag agcctccagg aatcgtacgg
     6061 tccgaacacc gtctgcttcg gctgcggccc ctccaacgag aagggcctca agatccggag
     6121 cttcgcccag ggcgatgagg tcgtcgcgac ctggcaggcc gagccgcacc acctcgcgtt
     6181 cgagggcgtc ctgaacggcg ggatcatcgg cgcgctcctc gactgccaca gcaactggac
     6241 ggccgcgtgg cacctcatgc agaaggccag ggacggggtg ctgccctcga cggtcaccgc
     6301 ggacttccac gtcgtgctca agcgcccgac gccgatcgcc gagcccctcc acctgaaggc
     6361 gcacgtcgtc gagtcgacgg cggaccgcgc cgtcgtcgaa gcgacgatcg aggcgggggg
     6421 caaggtcacg gcgacgtgcc gcggcacgtt cgtcgcggtg aaggaagggc acccggcgtt
     6481 ccaccgctgg tgaccggagc cgccgcgccc gccgcgcccg ccgcgccccc gtcgcgcacg
     6541 ttcgtcgcgc tcttcttcct gctcgtcctc gcggcgcttc tcttctcgga caagggccag
     6601 atcgcgggcg acggcgaggt gcgctggggg gcgctcgttg cgctcatgga cgaggggcgg
     6661 cacacgcccg accgttacac gctcgagatg ccgatcgccg cggcgccgct ctgggcggcc
     6721 ggcgcggcgg cagcccgcgt ggagggcgcg cccggcgccg cccgcctcga cacgatccgc
     6781 cgcgtcgtcc agcggttcaa caagctcgtc gccttcgcga tcgccgtctg gctcttccgc
     6841 cgactccggg gtctcggcct cgacgagttc gctgccgcgg gcagcgttct gatccttctc
     6901 ttcgccagcc ttctcgttcc tcattcgaag gacttctact ccgaaagcct ctggacgctg
     6961 ctcgcgtgcg tggcgctggg ccaactcgcg gacgggccgg cgcggacgcg cgccggcgac
     7021 gccgcactcg cggtggcggc ggcgcttgcg ataccgctga acccgctcct ttgtcctgtg
     7081 atcttcgtga cgtcggccat gctgtttttc ttagaaggtg aagaggggcg atccgccgcg
     7141 cgccggggtg cggttctcgc ttcggccggc gccgccctgg gtcttggtct tgcgctttct
     7201 gagaatcttc tccgccgcgg tgcgctcctc gatttcggtt acgcgggaga aggcttcacg
     7261 gcacccttct tcgaggggct cttcggccag ctcttctccc cggcccgcgg cgccctcttc
     7321 tacctgccgc tcttcttcgt cggcatcctc ctcctcgggc gtcgcgcccg cccggacgtg
     7381 cggcgcttca cccttgcggg ggcgacgttc ggcgtccttc tcgtcctcgc ctacgcaaag
     7441 tggcacgcgt ggcacgggat gacgtactgg gggccgcgct tcctcctgcc gctctcgatc
     7501 ttcggcgcgg cgttcgtcgc cctcgcgtgg cgggagtctc cgcggccgtc ctggcgcacg
     7561 gccctcgtcc ttctcgtcgc gatctcgtac gcggcctaca aggtcggcgt cggtgtcggc
     7621 gtcgtgccgc tccgggcgtg cctcctgccc gaccccgcgc gcctctcgtg ctactgggac
     7681 tgggcccggc tgccgctcgc ctcctggctc tcaccgggag acctcgcgtc gatgctctcc
     7741 catcgctcga cggcggtgga ggcgggcacg ctcgtcctct tcgcggtcct cgtcgccctc
     7801 acggggcgag gtcgatcacg acgcgcgatt ttcccgcagg gaccggcacc ccgatcaggt
     7861 tgaggtcggc cgcgtagacc gtcgcggact ttccgtccac ggaggccgtg gcgcgcggcg
     7921 tgaagctgcg gtcgacgcgc aggatcccgg cgcgcgcgcc cgacgtctcg acgacgaggc
     7981 ggtccgctcc gtcctcgacg aagcgcgcct cggccgcggc gtcgcgcacg gatgaagaga
     8041 cggatgcggc gtcgcgggcg tggacgacga cgtccgtggc cgggtcgaac cccgcgtcct
     8101 cgaacgcgga gacggtcgcg ttgatggagc cggcgacgac gattcggccc gcacgccgga
     8161 ggcccggcag cggatccgtc acgcgccaga gcgtgaccgg gacgcccgcg gagccctcca
     8221 cgtagacggg cgtgaggccg ggtgtttccg gcgggacgtc ggccgcgatc acggcacgca
     8281 cgcccgcggc gcgcagccac ttcaggcgcc ggccccacgg agcctgcttc acgacgtcgg
     8341 acgcgtagcg gttcaggatc gtgtagctgc cgtcgggatc cgggtcccac gcgtaggcga
     8401 ggccgtgcgg cgctcccgac agcgcccagc cctgcctcgc ctgtgcgagc gagaggtcga
     8461 ggatgtcgcc tgtcacgacg cgcccctgcg ccccgcgccg gacggcgtcg aagtccttcg
     8521 cggcgcgctc gtaaacgcgg cctccgagcg cggccgcacg ggcgacgagc ggcgacggct
     8581 cccggaagat ctcggacggg acgcgcggga tcgccgtggg cgtgtaggcc agcagagaaa
     8641 ccgccgtcgc ggcgaggagc aggccctggc cgcgggcgtc tcgcgccccg cgcgcgagga
     8701 ggagcccgag cacggcgagc ccggcgcccg cgagggccgc ctcggaggga atccgctcga
     8761 gaacgggagc gaggacgatg ccgggcggtg acgcccacgc ggggtcccag agccgcacga
     8821 ggacggcgaa aaggatgcgc ggtgaaaggc gcgtgacgat ggcaaggagg ccgaagagca
     8881 ccgcgagccc gagaagcgct cgagacgcgc gccgccggaa gcgcggcagt gcctcctcga
     8941 cgagcaggcg gtcgacgccg agcgcggcga gggcggcgag cgccatcgtg aacggcagga
     9001 gcgctttcac cgggtagcgc aggacgtgga gggccggcag cgcctcgtag gcggcgcg
//
LOCUS       DAOMFY010000148         8970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2372_length_8970_cov_8.149748, whole genome shotgun sequence.
ACCESSION   DAOMFY010000148 DAOMFY010000000
VERSION     DAOMFY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_8970_cov_8.149748"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLB01_12185"
     CDS             <1..722
                     /locus_tag="PLB01_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78103.1"
                     /translation="CDAARGVPVTRRGVPAALAATLLLGACGSSNEVKLKLPLRPKLA
                     VTGRERITLAPFIVASRLSDKKDAPKYKDLDLDAEFRRYLGKQLGKRTKMTIVTMPAD
                     VRLPTTHLKELTEAGDFWKGLGARTGADIILSGVVDFRVENKSGYRSEEYISPIDGRK
                     YYRQVSIDSTGFSFDVTVLAMDGRTGARLFQENFRDTKEKNQRSVNELAGLFENLFSL
                     ENQLLGLFVVREREANRYVFD"
     gene            709..3597
                     /locus_tag="PLB01_12190"
     CDS             709..3597
                     /locus_tag="PLB01_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78104.1"
                     /translation="MSSTRRVLALALLVGVAAAAAPRVHAAGQNKIVYDTFDWSIYQS
                     THFDVYHYSREKATLQKVVSMAESAYDELSRRLNYQIPKRVPIIYYATHAEFEQNNIE
                     LNFIPEGVGAFAEPARNRMVLPIDLPDEKLQALIQHELTHVFQYEILFGGRLSRALTS
                     NPPTWFMEGMASYFGNDEDDKDRMYLRDAVNADLIPPITKVQISGYPAYRYGHAVFDF
                     IESEWGKDGVRDFIFEFRSFLGRDISVALRRTFEIQPDDFDLKFRRYLRRKYLPLLAV
                     KGEASEYGERFRVGPYGRESYEIGAAPSPSGDFIATLTTYHDDIDIGLLSVKERKLFK
                     NLSPGTTTKYEYIVGQYLTTGPESGRDLSFAPDGDRIAVFGRQERGRKLLIFSAKGGG
                     LLERIAVSPDVPLSPSWAPSGNEIVFSAIEGNSRDIFLLDLKTRAVKNLTNDAAYDEG
                     PVFSPDGRYVYHSKIVGARSKIVRFPLSDPSKVEQVTWGDGSDEDPAFSPDGKRLYYA
                     SSRGGIFNVYGQDLATGEIAQYTDVIGAALGPAAFTGPDGQEKVVFSGYQGGRFQLYM
                     AEAKKPFRKLDEKAAAPAPLKPDGTPGFVPSVEVSVDPEKISRPKFKLFLDNASLIAG
                     VNSDQTFVSQILLSFSDYLGDRRAFFQFDSVSTFSNFRIGYFNLRRRLQWGAQIYDSR
                     SYFYGYDYNTGATVRDQRIARDTGVTLMGTYPISRYTRFDGNIGFVSRSLDVPILQSN
                     ADGSQQYVYLPRSDDVPTAGVGFSNDTTRYSYFGPLSGHRLDVNLQYTPSFQTGETTL
                     SFDAFAEARLYVPLSRRVLFAFRAFAAHSNGAAPTVYAFGGLDTLRGYDYNTVIGNNV
                     FYVNSEFRFPLIDVLATPILAFQGVRGRIFLDVGGGWFEGQSFVFMTDGQLNGLSNPS
                     IPGGLASFGGGFSVYFLGLPWNVDFAKKWDFKNTLSDWQTTFYVGTTF"
     gene            3733..4494
                     /locus_tag="PLB01_12195"
     CDS             3733..4494
                     /locus_tag="PLB01_12195"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HQQ78105.1"
                     /translation="MRIRVLGAHGGSTPRHRQTSFLVNDALCVDAGAVTDALTLEQQE
                     RVRAVLVTHSHMDHVASLPFLVENVFGRTRGPLEVLAPDDVLDALRTHLFNDALWPDF
                     SRIEGQAGPSVTFRAVPVGTPFSADGLTATAVKVSHVVPTYGYVLADERSAVVFSGDT
                     GPTEAIWSAARRTPNLKALFVECSFPSDMQPIADVSCHLTPKTLRAEMAKFPGGVPVY
                     LYHMKPPALDRLAAEIAAFGDSRLRVLADGDDLVF"
     gene            complement(4491..5216)
                     /locus_tag="PLB01_12200"
     CDS             complement(4491..5216)
                     /locus_tag="PLB01_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78106.1"
                     /translation="MNAAEPHRALIARVEGRRLLADAGFPLPVLIPLQLPLKEIPSSF
                     GTLTVAAAEDGGEGSGDLRVTCDARGEVAELLRLGPGEGGVGARADATPSATRVLEDR
                     VLHWSGGVMTVLDAWSVLRYPLAGTEQAAFEALFALNLEGVDLPAVPAGEFAPALTVF
                     HTVPLPPEDVQRGLAGADRPSSDLVAAPEATIRAVPGGSLVARSARFLAPVPATGPGE
                     SVRKTLVFHLAMDLLRLGAAAGS"
     gene            complement(5587..6375)
                     /locus_tag="PLB01_12205"
     CDS             complement(5587..6375)
                     /locus_tag="PLB01_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQQ78107.1"
                     /translation="MSSEFSAATIDRPTDVLVEDRGAIRILSLSRGKANALSTEFAHA
                     LHNAALAAQEDPFVRGVVLTSTNPKIFSAGFDLRKLAHATDEEFARFVRVFDSLFFDL
                     FLLGKPLVVALTGHAVAGGALLASTADFRIAAEGPGTIGLPEVTLGIHVPRHYLEAMR
                     TTVGEQALTRWALLGETMPFAEAHELGAIDRIVPADRLIEEAVSFAQRLGGASSEVYA
                     AIKRDLRGTAHERALDIQPDARKAFVESWFSETGQRGLDRALAH"
     gene            complement(6372..6959)
                     /gene="pdxT"
                     /locus_tag="PLB01_12210"
     CDS             complement(6372..6959)
                     /gene="pdxT"
                     /locus_tag="PLB01_12210"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HQQ78108.1"
                     /translation="MIGVLALQGDFEAHLAALARAGVAARPVRSAADVRATTGLVIPG
                     GESTTFRKLMEGTGIEEAIREVAFRGDPVLGTCAGAILLAEAVTNPDASGLGLLPIGI
                     ARNAYGRQLDSTVLRLTDVDRAALGDAPLEGVFIRAPKITAAGPGVEVLARRDGDPVL
                     VRKENVLAATFHPELTDDRRVFDLFLAARMRTSAR"
     gene            complement(6965..7873)
                     /gene="pdxS"
                     /locus_tag="PLB01_12215"
     CDS             complement(6965..7873)
                     /gene="pdxS"
                     /locus_tag="PLB01_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HQQ78109.1"
                     /translation="MTTPVFSSPFSSDAFRVKVGLAEMLKGGVIMDVVNAEQAKVAED
                     AGAAAVMALERVPADIRRDGGVARMSPVSKIREIEAAVSIPVMAKCRIGHLAEARILE
                     ALGVDFIDESEVLTPADEENHVYKHDYKVPFVCGCRNLGEALRRIGEGAAMIRTKGEA
                     GTGDIVHAVKHLREVTRQMKALTLLTKDEWMTAAKNLQAPFDLVVWVAQHGRLPVPNF
                     AAGGIATPADAALCRMLGAEAVFVGSGIFKSDDPARRARAIVEATTHFEDADRVAKAS
                     EALGEGMKGIEAAKLPGAEQLQTRGW"
     gene            complement(7891..>8970)
                     /locus_tag="PLB01_12220"
     CDS             complement(7891..>8970)
                     /locus_tag="PLB01_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQQ78110.1"
                     /translation="GGKDAGGVERLYRPLRETLAERLSRRGIAADPDALLIVNGAQQG
                     LDLITRALVDPGDAVAVESPSYSGALSLLRAHRARLLGVPLDAEGMEPGAFERALREG
                     PKFVYAIPDFHNPTGLLASAPRRAEIAGRAAAAGVLLVEDAFDADLLVEGELPPPLAA
                     LAPEHVIHLGTISKALFPGVRVGWIAGPKEFVHRLAALKRVGELTSPPVLQAAVNLFL
                     TRGDYDRHLARTRLRLKGRLALAHRTVVNRFPIGTAVERPAGGWVLWVRLPEGVPGRA
                     LAAAAREQGVLLSAGADFLPDQSDVSAIRVSVARGTAGELEKALEIAGASARELARQA
                     ARQKKGRARAARDASDAKEAALIQI"
BASE COUNT         1342 a         3271 c         3024 g         1333 t
ORIGIN      
        1 cctgcgacgc cgcccggggg gttcctgtga cgcgccgggg cgtcccggcc gccctcgccg
       61 cgacgctcct tctcggggcc tgcggaagct cgaacgaggt caagctcaag ctccccctcc
      121 gcccgaagct cgccgtgacg ggccgcgagc gcatcacgtt ggctcctttc atcgtcgcct
      181 cgcgcctgtc ggacaagaaa gacgctccga agtacaagga cctcgacctc gacgccgagt
      241 tccgccgcta tctcggcaag cagctcggca agcgcacgaa gatgacgatc gtcacgatgc
      301 cggccgacgt ccgtctcccg acgacgcacc tcaaggaact cacggaagcc ggagacttct
      361 ggaagggcct cggggcgcgc accggcgccg acatcatcct gtccggcgtc gtcgacttcc
      421 gcgtcgagaa caagtccggc taccgctccg aggagtacat cagcccgatc gacggccgga
      481 agtactaccg gcaggtctcg atcgattcga cgggtttctc cttcgacgtc accgtcctcg
      541 cgatggatgg gcgaaccggc gcgcgcctct tccaggagaa cttccgcgac acgaaggaga
      601 agaaccagcg ctccgtgaac gagctcgccg ggctcttcga gaacctcttc tccctcgaga
      661 accagctcct cgggctcttc gtcgtccgcg agcgggaggc caatcgctat gtcttcgact
      721 aggcgggtcc tcgccctcgc actcctcgtc ggcgtcgcgg cggccgcggc tccgcgcgtc
      781 cacgccgccg gccagaacaa gatcgtctac gacacgttcg actggtcgat ctaccagtcg
      841 acgcacttcg acgtctacca ctactcgcgc gagaaggcga cgctccagaa ggtcgtctcg
      901 atggccgagt ccgcctacga cgagctttcg cgccggctga actaccagat cccgaagcgc
      961 gtcccgatca tctactacgc gacgcacgcg gagttcgagc agaacaacat cgagctgaac
     1021 ttcatccccg agggcgtcgg cgcgttcgcg gagcccgcgc gcaaccggat ggtcctcccg
     1081 atcgacctgc cggacgagaa gctccaggcc ctcatccagc acgagctcac gcacgtcttc
     1141 cagtacgaga tcctcttcgg cggccgcctc tcgcgcgccc tcacctcgaa cccgccgacg
     1201 tggttcatgg agggcatggc ctcgtacttc ggcaacgacg aggacgacaa ggaccggatg
     1261 tacctccggg acgccgtgaa cgcggacctc atcccgccga tcacgaaggt ccagatcagc
     1321 ggctatcccg cgtaccgcta cggccacgcc gtcttcgatt tcatcgagtc cgagtggggc
     1381 aaggacggcg tccgcgactt catcttcgag ttccggtcgt tcctcggtcg ggacatctcc
     1441 gtcgcgctcc gccggacctt cgagatccag cccgacgact tcgacctgaa gttccggagg
     1501 tacctgcgcc gcaagtacct tcccctcctc gcggtcaagg gcgaggcctc cgagtacggc
     1561 gagcgcttcc gcgtggggcc gtacggccgc gagtcctacg agatcggggc ggcgccttcg
     1621 ccgtcgggcg acttcatcgc gacgctcacg acctaccacg acgacatcga catcgggctg
     1681 ctctccgtca aggagcgcaa gctcttcaag aacctctctc ccgggacgac gacgaagtac
     1741 gaatacatcg tcgggcagta cctcacgacg gggcccgaaa gcgggcgcga cctctccttc
     1801 gcgccggacg gggaccgcat cgcggtcttc ggccggcagg agcgcggccg caagctcctg
     1861 atcttctccg cgaagggcgg cggcctcctc gagcgcatcg cggtttcacc ggacgttccg
     1921 ctttcgccgt cgtgggcccc ctcgggcaac gagatcgtct tctcggcgat cgagggaaac
     1981 agccgggaca tcttcctcct cgacctcaag acccgcgccg tgaagaacct cacgaacgac
     2041 gccgcctacg acgaggggcc ggtcttctcg cccgacggcc ggtacgtcta ccactcgaag
     2101 atcgtgggcg cccgctcgaa gatcgtccgc tttccgctct cggatccttc gaaggtcgag
     2161 caggtcacgt ggggcgacgg cagcgacgaa gacccggcgt tctcgcccga cggcaagcgg
     2221 ctctactacg cctcgtcccg cggcggcatc ttcaacgtct acgggcagga cctcgcgacg
     2281 ggcgagatcg cccagtacac ggacgtcatc ggcgccgcac tcgggccggc cgcgttcacc
     2341 gggccggacg ggcaggagaa ggtcgtgttc tcgggctatc agggcgggcg gttccagctc
     2401 tacatggcgg aagcgaagaa gccgttccgc aagctcgacg agaaggctgc ggcgcccgcg
     2461 ccgctgaaac ccgacgggac gcccggtttc gtgccgtccg tcgaggtctc cgtcgacccc
     2521 gagaagatct cgcgcccgaa gttcaagctc ttcctcgaca acgcgtccct catcgccggc
     2581 gtcaactcgg accagacgtt cgtctcgcag atcctcctgt cgttctccga ctacctcggc
     2641 gaccgccgcg ccttcttcca gttcgactcg gtgtcgacgt tctcgaactt ccggatcggc
     2701 tacttcaatc tccgccggcg tctccagtgg ggcgcgcaga tctacgacag ccgcagctac
     2761 ttctacggct acgactacaa caccggcgcc accgtgaggg accagcggat cgcgcgggac
     2821 acgggcgtga ccctcatggg cacctacccg atctcgcgct acacgcgctt cgacggaaac
     2881 atcggattcg tgagccgctc gctcgacgtc cccatcctcc agtcgaacgc ggacggctcc
     2941 cagcagtacg tctacctgcc ccggtcggac gacgttccga cggccggcgt gggcttctcg
     3001 aacgatacga cgcgctactc gtacttcggg ccgctctcgg ggcaccggct cgacgtgaac
     3061 ctccagtaca cgccgtcctt ccagacgggc gagacgacgc tctcgttcga cgcgttcgcg
     3121 gaggcccgcc tctacgtccc gctctcgcgg cgcgtcctct tcgcgttccg cgccttcgcc
     3181 gcgcactcga acggggcggc gcccaccgta tacgcgttcg gcgggctcga cacgctccgc
     3241 ggctacgact acaacacggt catcggcaac aacgttttct acgtcaacag cgagttccgt
     3301 tttcccctca tcgacgtcct cgcgacgccg atcctcgcgt tccagggcgt ccgcggccgc
     3361 atctttctcg acgtcggcgg cggctggttc gaaggccaga gcttcgtctt catgacggac
     3421 ggccagctca acgggctgag caacccgagc attccgggcg gtctcgcgtc tttcggtggc
     3481 ggcttctcgg tctacttcct gggtctgccg tggaacgtgg actttgccaa gaagtgggac
     3541 ttcaagaaca cgctgtcgga ctggcagacg accttctacg tcgggacgac cttctagagg
     3601 gggacccggc cccgggtccc cctcgcggct tcgccgctcc ccctccgcgg cggaatcgcc
     3661 ggccttcgag ctttgtgagg ccggtcacgc gcggtccgca tcgtacctcc ccggcctcat
     3721 aatccccgac ccgtgcgcat tcgcgttctc ggagcccacg gcggctccac gccccgacac
     3781 cggcagacgt ccttcctcgt gaacgacgcg ctctgcgtgg atgcgggagc ggtcacggac
     3841 gcgctgacgc tcgagcagca ggagcgcgtg cgcgccgtcc tcgtcacgca ctcgcacatg
     3901 gaccacgtcg cctcgcttcc gtttctcgtc gagaacgtct tcgggcggac gcgcggaccc
     3961 ctcgaggtcc ttgcgcccga cgacgtcctc gacgccctcc ggacgcacct cttcaacgac
     4021 gcgctgtggc cggacttctc gcgcatcgag ggccaggccg gcccgagcgt cacgtttcgc
     4081 gccgttccgg tcgggacccc gttctccgcc gacggcctca cggcgaccgc cgtgaaggtg
     4141 tcgcacgtcg tcccgacgta tggctacgtc ctcgccgacg agcgctccgc cgtcgtcttc
     4201 tccggcgaca ccggccccac ggaggccatc tggtccgcgg cgcgccgcac gccgaacctc
     4261 aaggcgctct tcgtcgagtg ctcgttcccg agcgacatgc agccgatcgc cgacgtctcc
     4321 tgccacctga cgccgaagac gctgcgcgcc gagatggcga agttccccgg gggcgtcccc
     4381 gtctacctgt accacatgaa gccgcccgcc ctcgatcgcc tcgccgccga gatcgccgcg
     4441 ttcggagact cccgcctgcg cgtcctcgcg gacggcgacg acctcgtctt ctagcttccc
     4501 gcggccgcgc cgaggcgcag gagatccatc gcgaggtgga agacgagcgt cttgcggacg
     4561 gactcgccgg gtcccgtcgc gggcaccggc gcgaggaacc gggcgctccg cgcgacgaga
     4621 gaaccgcccg gcacggcgcg gatcgttgct tcgggcgccg cgacgagatc ggacgacggc
     4681 cgatcggcgc cggcgagccc acgctgcacg tcctccggcg gcagcggcac cgtgtggaag
     4741 accgtgaggg cgggcgcgaa ctcccccgcc gggacggcgg gcaggtcgac gccctcgagg
     4801 ttcagggcga agagcgcctc gaaggccgcc tgctccgtcc ccgcgagcgg atagcgcagg
     4861 acactccacg cgtcgaggac cgtcatgacc ccgcccgacc agtggaggac acggtcctca
     4921 aggacgcgcg tggcggaggg ggtggcgtcg gcgcgcgcgc cgacgccgcc ctcccccggc
     4981 cccagtcgga ggagctcggc gacttcaccg cgcgcgtcgc acgtgacccg aaggtcgccg
     5041 ctgccctcgc ccccgtcctc ggcagcggcg acggtcaggg tgccgaagga agaggggatt
     5101 tcttttaaag gtaattgaag agggatgagc acgggcaacg ggaacccggc gtccgcgagg
     5161 aggcgccggc cctccacgcg cgcgatgagg gcgcgatgag gttctgccgc attcaccttc
     5221 aaagaaaaac caccggcagc ctcatcgggg cgcacgtcgt cgcgcaccgt ccgcgcccac
     5281 ggtcgggagc aaagagactc ttcgaggctg cattcgaacc cgacctcccc cgcgagccac
     5341 gcgaaggccc gcgcgcgctc ctcccccgtc agcccgcgct cctcgtccgg ccacgcggcc
     5401 gcaaactcct ccgcgtcgaa gagggcgggg ggggccgcct cccgcgtgag cgtctcgcac
     5461 gcgacgcggc gctggaagac gtggaggaga tcttcgagaa agcgcgtgtc ggggagccgc
     5521 gcttcgaggc cgagcgcctc gagccacgcg cgcgcgcgag gggtcgtcag gccgcctccg
     5581 cccgcgtcag tgcgcgaggg cgcgatcgag cccccgctgg cccgtctcgg agaaccagga
     5641 ctcgacgaac gccttgcgcg cgtcgggctg gatgtccagg gcgcgctcgt gcgccgtccc
     5701 gcgcaggtcg cgcttgatcg cggcgtagac ctcgctcgac gcgccgccga gccgctgggc
     5761 gaaggacacg gcttcctcga tcagccggtc ggcgggcacg atccggtcga tggcgccgag
     5821 ctcgtgcgct tccgcgaacg gcatcgtctc gccgaggagc gcccaccgcg tgagcgcctg
     5881 ctcgccgacg gtcgtccgca tcgcctcgag atagtggcgc gggacgtgga tgccgagcgt
     5941 gacctcgggg aggccgatcg tgcccggccc ctcggccgcg atccggaagt ccgccgtcga
     6001 ggcgagaagc gcgccgcccg cgaccgcgtg gcccgtgagg gcgacgacga gcggtttgcc
     6061 gaggaggaag aggtcgaaga agagcgagtc gaagacgcgc acgaagcggg cgaactcctc
     6121 gtccgtcgcg tgcgcgagct tcctgaggtc gaagcccgcc gagaagatct tcgggttcgt
     6181 cgacgtgagc acgacgccgc ggacgaacgg gtcctcctga gcggcaaggg cggcgttgtg
     6241 gagcgcgtgc gcgaattccg tcgagagggc gttcgccttc ccgcgcgaga gcgagaggat
     6301 gcggatcgcc ccccggtcct cgacgaggac gtcggtcggc cggtcgatcg tggcggcgga
     6361 aaactcggaa ctcatcgcgc cgatgttctc atgcgggcgg cgaggaagag gtcgaaaacc
     6421 cggcggtcgt ccgtgagctc cgggtgaaac gtcgccgcga ggacgttctc cttccggacg
     6481 agcaccgggt cgccgtcccg gcgcgcgagg acctcgacgc ccggcccggc ggccgtgatc
     6541 ttcggcgcgc ggatgaacac gccctcgagc ggcgcgtctc cgagggccgc gcggtccacg
     6601 tccgtgaggc gaaggaccgt cgagtccagc tgccgcccgt aagcgttccg cgcgatcccg
     6661 atcggcagga ggccgaggcc gctcgcgtcg gggttcgtca cggcctcggc gaggaggatc
     6721 gctcccgcgc aggtcccgag caccgggtcg ccgcgaaacg caacctcgcg gatggcctcc
     6781 tcgatcccgg tcccctccat gagcttgcgg aaggtcgtcg actccccgcc cgggatcacg
     6841 aggcccgtcg tggcgcgcac gtccgccgcc gagcgcacgg gccgggcggc gacgccggcc
     6901 cgggccagcg ccgcgaggtg tgcctcgaag tcgccctgga gggcgaggac gccgatcacg
     6961 ggacctacca gccgcgggtc tggagctgct cggccccggg aagcttcgcc gcctcgatcc
     7021 ccttcatccc ctcgccgagc gcctcggacg ccttcgcgac gcggtcggcg tcctcgaagt
     7081 gcgtcgtggc ctcgacgatc gcgcgcgcgc gcctggcggg atcgtccgac ttgaagatcc
     7141 ccgacccgac gaagaccgcc tcggcgccga gcatccggca gagcgccgcg tcggcgggcg
     7201 tcgcgatgcc gccggccgcg aagttcggga ccgggagccg gccgtgctgc gcgacccaca
     7261 cgacgaggtc gaacggagcc tggaggttct tggcggccgt catccactcg tccttcgtga
     7321 ggagggtgag cgctttcatc tggcgcgtga cctcgcgcag atgcttgacg gcgtgaacga
     7381 tgtcgcccgt gccggcctcg cccttcgtgc ggatcatcgc ggcgccctct ccgatccggc
     7441 gcagcgcctc accgaggttc cggcacccgc agacgaacgg gaccttgtag tcgtgcttgt
     7501 agacgtggtt ctcctcgtcg gccggcgtga ggacctcgga ttcgtcgatg aagtccacgc
     7561 cgagggcctc gaggatccgc gcctcggcca ggtggccgat gcggcacttg gccatgacgg
     7621 gaatcgagac ggccgcctcg atctcgcgga tcttcgagac gggcgacatg cgggcgacgc
     7681 cgccgtcgcg gcggatgtcg gcggggacgc gctcgagcgc catcacggcg gccgcgccgg
     7741 cgtcctcggc gaccttcgcc tgctcggcgt tgacgacgtc catgatgacg ccgcccttga
     7801 gcatctcggc gaggccgacc ttgacgcgga acgcgtcgga ggagaacggg gacgagaaca
     7861 cgggagtggt catcggagtt cctttcgtct tcagatctgg atgagggccg cttccttggc
     7921 gtcgctcgcg tcccgcgcgg ccctcgcgcg gcccttcttc tggcgcgcgg cctggcgggc
     7981 gagctcgcgc gcgctggcgc ccgcgatctc gagggccttc tcgagctcgc cggccgtccc
     8041 gcgcgcgacg gaaacgcgga tggcggacac gtcggactgg tcgggcagga agtcggcgcc
     8101 ggccgacagg aggacgccct gctcgcgcgc ggccgccgcg agcgcgcgcc cgggcacgcc
     8161 ctcgggcagg cgcacccaca ggacccaacc gcccgcaggg cgctcgacgg ccgtgccgat
     8221 cgggaagcgg ttcacgaccg tgcggtgggc gagggcgaga cggcccttca gccggaggcg
     8281 cgtcctggcg aggtggcggt cgtagtcgcc gcgcgtcagg aagaggttca cggccgcctg
     8341 caggacgggc ggtgacgtga gctcgccgac gcgcttgagc gccgcgaggc ggtggacgaa
     8401 ttccttcggg cccgcgatcc agcccacgcg cacgcccggg aagagcgcct tgctgatcgt
     8461 gccgagatgg atcacgtgct ccggcgcgag cgcggcgagc ggcggcggga gctcgccctc
     8521 gacgaggagg tccgcgtcga atgcgtcctc gacgagcagg acgcccgccg ccgcggcgcg
     8581 gcccgcgatc tcggcacgcc gcggcgccga cgcgaggagg cccgtggggt tgtggaagtc
     8641 cgggatcgcg tagacgaact tcggcccctc gcggagcgcc ctctcgaacg cgcccggctc
     8701 catgccctcc gcgtcgagcg gaacgccgag gaggcgcgcc cggtgcgcgc gcaggagcga
     8761 cagcgcgccc gaatagctcg ggctctccac ggcgacggca tcgccggggt cgacgagcgc
     8821 gcgcgtgatg aggtcgagcc cctgctgggc gccgttcacg atgaggagcg cgtcggggtc
     8881 ggccgcgatc ccccgccggg acaggcgttc cgcgagcgtc tcgcgcagcg gccggtacaa
     8941 gcgctcgacc ccgcctgcat ccttgccgcc
//
LOCUS       DAOMFY010000149         8950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2381_length_8950_cov_8.383811, whole genome shotgun sequence.
ACCESSION   DAOMFY010000149 DAOMFY010000000
VERSION     DAOMFY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2381_length_8950_cov_8.383811"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLB01_12225"
     CDS             <1..747
                     /locus_tag="PLB01_12225"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQQ78111.1"
                     /translation="ALALSGRVPAAVAVAVNALCAYAQFTVAHDAAHGSVSRLRALND
                     AAGTVAALALAAPFAAFRRIHLHHHAHVNDPAEDPDFWVVGKTWPETLARFATTWLHY
                     YRVYFLRLRALDAGLAVSVGTVFAFAAAGVLAFRAGRLADLVLYWFLPAQLAVTLLAF
                     LFDYLPHRPHTLRGRMVDTVSVTDRWLDAPFLGQNLHLLHHLFPSIPWYRYRPAWDEA
                     EEGIRAEGGVARTWREALATLPPSWRGSRA"
     gene            744..2114
                     /locus_tag="PLB01_12230"
     CDS             744..2114
                     /locus_tag="PLB01_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQQ78112.1"
                     /translation="MKIPVHVLSGFLGVGKTTAVKDLLARRADRERIAVVVNEFGTLG
                     VDGALLSDCASCILKEVPGGCVCCTAMADLEASLEEVCDIVAPTRFVLEPTGLARPSE
                     LVDLLRGPRWAPRFDVRPVITLLDPQQDLAKAYAESALFRDQVDVADVVVVNRCDLAS
                     EEEILRVEEFARALAPPKLRIVRTSHGVLPDEIWDLERKTPAAAPALLEASSSHAHAH
                     GDFLEGYVGRGVAEPPERIFDAERLLAALEELRSGRLTRGAVARAKGLFHTTAGWRVH
                     EIAGGRLSTAPTSWRRDSRVDVILKNPGEGDFLEWEEALGGALVPADAPLLTLEDGKG
                     AMRTYDPRGFSALSQSLSKKIFSSSSFPAFRLGDVLAHAGLAREADWMWIVMDSGLFG
                     VGGPRRVLENGIVFPGEEGFRFVLPDEAARGAEDDVDACRDLSNVCGVKLADSPGAEA
                     TAGEVS"
     gene            2111..3166
                     /locus_tag="PLB01_12235"
     CDS             2111..3166
                     /locus_tag="PLB01_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HQQ78113.1"
                     /translation="MTAKSGLIVYGANGYTGKLIVDVALREGLRPVVAGRRREAVEPL
                     AAAWGVPSLCFSLDDAASAAHLLEPYGAMILAAGPFSKTSAAALEACLRAKTAYLDIT
                     GEVDVFEAVFARDADAKKAGIAVLPGAGFDVVPSDCLAKALSEALPGAETLTLAFRGF
                     KTSAGTMKTMLEGIPKGGLVREGGKLVRVPAAWKTIEVPLGAKTRLAMTIPWGDLSTA
                     FRSTGIPNIEVYMAVPPSAVAAARRMRRFAKLLGLSPVQSFLKARVEKTVKGPTAEER
                     DRERSYLWGRVTRGGSAVTGKLETLEGYALTAETAVAIAKRVLAGDVGPGVHTPSQAF
                     GSRFIETIRGSRLTVPA"
     gene            3163..4314
                     /locus_tag="PLB01_12240"
     CDS             3163..4314
                     /locus_tag="PLB01_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQQ78114.1"
                     /translation="MIPVRMAVRHVTSRPLRSALTVAAIGLSAGLVGFLYLVNGALKN
                     DWSENMGFRAMVMAKTSFFERLPMAYLAKIEDVPGVQRVCPFDFLMAQWKDDRPENAV
                     PFQAAPADAFVEVYREAKIPPDQIAAWKADPTGCVIGPILAKQHGWKVGDKVVLKAPI
                     PGGVVETTIRGIMAYKVDNGLYAHRKYFENLTRDTGQVGMFWILAKSRGDVPKITAEI
                     ERRLDNAPVPIRAVSEKQWQLMFMEMLGNVQALLGGIGLATAFTLFLITSNTLAMSAR
                     ERRGEAALLRILGFPRATVLRLLVLEGAFFGAAGAVLGLGFMRLFGYLIGAALAETQW
                     GPIGALLVPDPAMVLAVAGLAFLVAVVAALVPAINLSGRPVVSLAREGE"
     gene            4358..5305
                     /gene="pip"
                     /locus_tag="PLB01_12245"
     CDS             4358..5305
                     /gene="pip"
                     /locus_tag="PLB01_12245"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HQQ78115.1"
                     /translation="MPLLFPEIEPYRAFRLPVSEIHTLHVEESGNPDGAPVIFFHGGP
                     GAGLSPMHRRFFDPKFWRVVLFDQRGCGQSTPLGELRENTTWDLVADAERIRTHLGIA
                     KWLVFGGSWGSTLGLAYAETHPERVTGLVLRGIFLGRKLEIDWTFVDGLRRMEPDGWD
                     AFVAGLTPRERGAVVRAYYRKLTSDDAAVRREAALAWNRFENHASKLVPVEEPVAEED
                     VPKEIALARIEAHYFLNGSFLKSDDQLLHDAERLRRIPGVIVQGKYDLVCPMQSAWDL
                     HLAWPEARYVVVPDAGHSADEPGITSALVAATEDFKATV"
     gene            5307..5684
                     /locus_tag="PLB01_12250"
     CDS             5307..5684
                     /locus_tag="PLB01_12250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQQ78116.1"
                     /translation="MEAVFTTGAPAPAGHYAQGVVHGGLVYVAGQLGKDPARPDSGPG
                     SLEEQTERALRNVEAVLKAAGSDLAHVLQVTVYVSDASFWGRVNEVYARVMGDARPAR
                     AVVPVNEFRGGWQVEVAAVAALP"
     gene            5681..8170
                     /locus_tag="PLB01_12255"
     CDS             5681..8170
                     /locus_tag="PLB01_12255"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HQQ78117.1"
                     /translation="MISRRQAKDINADDVVLGLDAAAIRLSFVEKLFFEVAKFPGVAT
                     RNDHYLALARAVRDRILHRWIRSARTYLEEQHRTVIYLSAEYLMGPQLGNNLFNLGIE
                     GPARAAMESLGLNLDELIEHEEEPGLGNGGLGRLAACFMDSLATLDFPAIGHGIRYEF
                     GIFDQEIVDGWQRERTDRWLRTGNPWEVRRYEIEHPVGFGGYTQTVVDGSGRSRVKWT
                     PARLVRGVPYDTPVVGYGTVTTNFLRLWAAVAPEEFDLDAFQVGEYWRAVDEKIRSEN
                     ITKVLYPNDASTAGKELRLQQEYFFVSCALQDCIRLLLQRTTIDKFADKFAIQLNDTH
                     PALAVPELMRLLMDEHGLGWDEAWDITERSFNYTNHTLLPEALETWPLPLLGRLLPRH
                     LEIVFEINRRFLDAVRARFPGDDARLSRLSLIDERGEKSVRMAHLAAVASRRVNGVAE
                     LHSRLLRETVLADFAALWPERFTNVTNGVTPRRFVGLANPRLSSLVTEAIGDGWLRDL
                     ERLSGLETLASDAVFRERWRAVKQANKERLAGWLRETVGCAVDPHTLFDAQCKRIHEY
                     KRQQLNLLHVVWLYQRILRGDVAGMTPRTFLFAGKAAPGYHAAKLIIRLASAVGETLE
                     RHPVAKDLLSVVFVPDFNVKNAQRIYPAVDLSEQISTAGKEASGTGNMKFAMNGALTI
                     GTLDGANVEIRDAAGAENFFLFGLTAAGVEDVLRRGYKPRDVIESDRELADVLWLVES
                     GHLVPDEPDLFRPFVRTLVEEDPYLVLADFRAYVECQARVSAAWSDVVRWTSASILNV
                     ARMGRFSSDRSIRDYVREVWHVRPVSLAPVR"
     gene            8226..>8950
                     /locus_tag="PLB01_12260"
     CDS             8226..>8950
                     /locus_tag="PLB01_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HQQ78118.1"
                     /translation="MPESQQPLSLRLLSLYPKKSGSLAVGALARLGLPKRLRRPLLGR
                     FAAAYGANLDEAGLPLEEYASFLDFFTRRLKPGLRPQEPAVPGGINSPVDGALIASGR
                     VTEGTLLQAKGLPYRLDELLDGDPLASELAGAHYLTLYLAPKDYHRIHVPLTGACVSV
                     GRVEGELWPVNEASTRFTPRLYVRNRRAYWIARGAGPDEGLVAAAVLVAATHVGGVVI
                     DPRWLSGRTLPRRGRLDVAALPAA"
BASE COUNT         1267 a         3184 c         3097 g         1402 t
ORIGIN      
        1 gctctcgcgc tctccgggcg cgtcccggcg gcggtcgcgg tcgcggtgaa cgccctttgc
       61 gcgtacgcgc agttcacggt cgcccacgat gcggcgcacg ggtccgtctc gcgtctccgg
      121 gcgctcaacg acgccgcggg gacggtcgcc gcgctcgccc tcgccgcccc gttcgccgct
      181 ttccgccgga tccacctcca ccaccacgcc cacgtgaacg accccgccga ggaccccgac
      241 ttctgggtcg tggggaagac gtggcccgag acgctcgcgc gcttcgcgac gacgtggctg
      301 cactactacc gcgtctactt cctgcgcctg cgggccctcg acgcgggcct cgccgtttcg
      361 gtcgggacgg tcttcgcctt cgcggccgcc ggcgttctcg cgttccgcgc cgggcggctc
      421 gcggacctcg tcctctactg gttcctcccg gcgcagctcg ccgtgacgct cctcgcgttc
      481 ctctttgact acctcccgca ccggccgcac acgcttcggg ggcggatggt cgacacggtc
      541 agcgtgacgg accgctggct ggatgcgccg tttctcgggc agaacctcca cctcctccac
      601 cacctcttcc cgtcgattcc gtggtaccgc taccggccgg cctgggacga ggcggaggag
      661 ggcatccggg ccgagggcgg cgtcgcgcgc acgtggcgcg aggccctcgc gactctgccg
      721 ccgtcgtggc gggggagccg cgcgtgaaga tccccgtcca cgttctctcg ggattcctcg
      781 gcgtcggcaa gacgacggcc gtgaaggatc tcctcgcgcg gcgcgcggac cgcgagcgga
      841 tcgcggtcgt cgtgaacgag ttcgggacgc tcggcgtgga cggcgcgctc ctgtcggact
      901 gcgcgagctg catcctcaag gaagtcccgg gcggctgcgt gtgctgcacg gccatggccg
      961 acctcgaagc gtcgctcgag gaggtctgcg acatcgtggc cccgacgcgg ttcgtcctcg
     1021 agccgacggg cctcgcgagg ccgtccgagc tcgtggacct cctgcgcggg ccgcgctggg
     1081 cgccgcgctt cgacgtgagg cccgtgatca cgctcctcga cccgcagcag gacctggcga
     1141 aggcgtatgc ggagagcgcc ctgttcaggg accaggtgga cgtcgcggac gtggtcgtcg
     1201 tgaaccggtg cgacctggcc tcggaggaag agattcttcg agtggaagag ttcgcccggg
     1261 cgctggcgcc gccgaagctg cgaatcgtcc gcacgtcgca cggggtgctg ccggacgaaa
     1321 tctgggacct cgagcggaag acccccgcgg cggctccggc gctcctcgag gccagctcct
     1381 cgcacgccca cgctcatgga gatttcttag aagggtatgt gggccggggc gtggcggaac
     1441 cgccggagcg gattttcgac gcggaacgcc tgctcgcggc actcgaggag ctccgctccg
     1501 ggcggctgac tcgcggcgcc gtcgcgcggg ccaaggggct cttccacacg accgcgggct
     1561 ggcgggtcca cgagatcgcg gggggccggc tttcgacggc tccgacgtcc tggcgccgcg
     1621 acagccgggt ggatgtgatc ttgaagaatc cgggagaagg agatttctta gagtgggaag
     1681 aggcactggg cggagcgctg gtgcctgcgg atgccccgct cctgacgctc gaagacggga
     1741 agggcgcgat gcgcacatac gatccaaggg gcttttcggc cctctcgcag tccctttcca
     1801 agaaaatctt ctcttcttct tctttccccg catttcgcct cggtgacgtg ctggcgcacg
     1861 cgggtcttgc ccgcgaggcc gactggatgt ggatcgtgat ggactcgggc ctcttcggcg
     1921 tcggcggccc gcggcgcgtg ctcgagaacg gcatcgtctt tccgggggag gaaggcttcc
     1981 ggttcgtcct gcccgacgag gccgcgcgcg gcgccgagga cgacgtcgac gcctgccgcg
     2041 acctctcgaa cgtctgcggc gtgaagctgg cggattcgcc gggagcggag gccacggccg
     2101 gggaggtctc gtgacggcga agagcggtct gatcgtctac ggcgcgaacg gttacacggg
     2161 aaagctcatc gtggacgtcg cgctccgcga gggcctgagg cccgtcgtcg cggggcgccg
     2221 ccgcgaggcc gtcgagccgc tcgccgccgc gtggggcgta ccgagcctgt gtttctcgct
     2281 cgacgacgcg gcgtccgccg ctcacctcct cgagccgtac ggcgcgatga tcctcgcggc
     2341 cggcccgttt tcgaagacga gcgcggccgc gctcgaggcg tgcctgcgcg cgaagacggc
     2401 ctacctcgac atcacgggcg aggtggacgt cttcgaggcg gtgttcgccc gcgatgccga
     2461 cgcgaagaag gcaggcatcg cggtccttcc gggcgccgga ttcgacgtcg tgccgtcgga
     2521 ctgtctcgcg aaagccctct cggaggccct gccgggggcc gagacgctca cgctcgcgtt
     2581 ccggggcttc aagacgagcg ccggcacgat gaagacgatg ctcgagggca tcccgaaggg
     2641 cggcctcgtc cgtgagggcg gaaagctcgt ccgggtcccg gccgcgtgga agacgatcga
     2701 ggtcccgctc ggcgcgaaga cgcgcctcgc catgacgatc ccgtggggcg acctctcgac
     2761 ggcgtttcgt tcgaccggaa tcccgaacat cgaggtctac atggcggtgc cgccttcggc
     2821 cgtcgcagcg gcgcgccgca tgcgccggtt cgcgaaactc ctcggcctct cgcccgtaca
     2881 gtccttcctg aaggcgcgcg tggagaagac ggtcaagggc ccgacggccg aggaacgcga
     2941 ccgcgagcgg tcgtacctct ggggccgcgt cacgaggggc gggagcgccg tcaccgggaa
     3001 actcgagacg ctcgaaggct acgcgctgac cgccgagacc gccgtcgcga tcgcgaagcg
     3061 ggtcctcgcc ggggacgtcg gcccgggagt ccacacgccg tcgcaggcct tcggctcccg
     3121 gttcatcgag acgattcgcg gatcgaggct cacggtaccc gcatgatccc cgtccgcatg
     3181 gcggtccgac acgtcacgag ccgccccctg cggagtgcgc tcaccgtggc cgcgatcggc
     3241 ctttcggcag gcctggtcgg gttcctctac ctcgtgaacg gcgcgctcaa gaacgattgg
     3301 tccgagaaca tgggcttccg cgcgatggtc atggcgaaga cgtcgttttt cgagaggctg
     3361 cccatggcct acctcgcgaa aatcgaggac gtcccgggcg ttcagcgcgt ctgcccgttc
     3421 gacttcctca tggcgcagtg gaaggacgac cggcccgaga atgccgtgcc tttccaggcg
     3481 gcgcccgccg acgccttcgt cgaggtctac cgcgaagcca agattccacc cgaccagatc
     3541 gccgcgtgga aggcggaccc gacgggctgc gtcatcggac ccatcctcgc gaagcagcac
     3601 ggctggaagg tcggcgacaa ggtcgtcctc aaggcgccca tcccgggcgg cgtcgtcgag
     3661 acgacgatcc gggggatcat ggcctacaag gtcgacaacg gcctctacgc acaccggaag
     3721 tacttcgaga atctcacgcg cgacacgggc caggtcggca tgttttggat cctcgcgaag
     3781 tcgcggggcg acgttccgaa gatcacggcg gagatcgagc gccgcctcga caacgcgccc
     3841 gtcccgatcc gggccgtgag cgagaagcag tggcagctca tgttcatgga gatgctcgga
     3901 aacgtgcagg cgctcctcgg cgggatcggg ctcgcgacgg cgttcacgct gttcctgatc
     3961 acgagcaaca cgctcgcgat gtccgcgcgc gagcgccgcg gagaggcggc cctcctccgg
     4021 atcctcgggt tcccgcgcgc gaccgtcctc cgcctgctcg tgctcgaggg agcgtttttc
     4081 ggggccgccg gcgcggtcct cgggctcggc ttcatgcggc tcttcgggta cctcatcggg
     4141 gccgccctcg cggaaacgca atgggggccg atcggtgcgc tgctcgtccc ggacccggcg
     4201 atggtgctcg ccgtcgccgg actcgcgttt ctcgtcgccg tcgtggccgc gctcgtcccg
     4261 gcgatcaacc tgtcgggccg gcccgtcgtg agcctggcgc gcgaagggga gtaggggagc
     4321 cgcgagttct tcgggacggg ctaggtaaac taggtccatg ccgctgctct ttcccgagat
     4381 cgagccgtac cgggcgttcc ggctgcccgt ctcggagatc cacacgctgc atgtcgagga
     4441 gtccggaaac ccggacggcg cacccgtcat cttctttcac ggcgggccgg gggccggcct
     4501 ctcgccgatg caccggcgct tcttcgaccc gaaattctgg cgcgtcgtcc tcttcgacca
     4561 gcgcggctgc ggccagtcga cgcccctcgg cgagctccgg gagaacacga cgtgggacct
     4621 cgtcgcggac gccgagagaa tccggacgca tctcgggatc gcgaagtggc tcgtgttcgg
     4681 aggatcgtgg ggttccacgc tgggcctcgc gtacgccgaa acgcatcccg agcgcgtcac
     4741 ggggctcgtg ctgcgcggga tcttcctcgg ccggaagctc gagatcgact ggacgttcgt
     4801 ggacggcctg cggcgcatgg aaccggacgg ctgggacgcg ttcgtggccg gcctcacccc
     4861 gcgcgaacgc ggcgccgtcg tgcgcgccta ctaccgaaag ctcacgagcg acgacgccgc
     4921 cgtgaggcgc gaagcggcgc tcgcgtggaa ccggttcgag aaccacgcct cgaagctcgt
     4981 ccccgtggag gagcccgtcg ccgaggagga cgtcccgaag gaaatcgctc tcgcgcgcat
     5041 cgaggcccac tatttcctga acggctcctt cctgaagtcc gacgaccagc tcctgcacga
     5101 cgccgagcgc ctccgccgga ttccgggcgt catcgtccag ggcaagtacg acctcgtgtg
     5161 cccgatgcag tccgcgtggg acctgcacct cgcgtggccg gaggcgcggt acgtcgtcgt
     5221 tccggacgcg ggccattccg ccgacgagcc gggaatcacg tccgcgctcg tcgcggccac
     5281 ggaagacttc aaggcgacgg tctgacgtgg aagccgtctt cacgacgggc gcgccggcgc
     5341 ccgcaggaca ctacgcgcag ggcgtcgtcc acggcggcct cgtgtacgtc gcggggcaac
     5401 tcgggaaaga cccggcccgg cccgactccg gtcccgggtc gctcgaggaa cagacggagc
     5461 gcgcgctgcg caacgtcgag gcggtcctga aggccgccgg cagcgacctc gcgcacgtcc
     5521 tccaggtgac ggtctacgtc tccgacgcgt ccttctgggg gcgcgtgaac gaggtttacg
     5581 cgcgtgtgat gggcgacgcg cggcccgcgc gggccgtcgt tcccgtgaac gaattccggg
     5641 gcggctggca ggtcgaagtc gccgcggtcg cggcgctccc atgatttccc gacgccaggc
     5701 gaaggacatc aacgcggacg acgtcgtcct cggtctcgac gccgcggcca tccggctttc
     5761 gttcgtcgag aagctcttct tcgaggtcgc gaagttcccg ggtgtcgcga cgcggaacga
     5821 ccactacctc gcgctcgccc gcgccgtgcg cgaccggatc ctgcaccggt ggatccgctc
     5881 ggcgcgcacg tatctcgagg aacagcaccg aacggtcatc tacctttccg ccgagtacct
     5941 catggggccg cagctcggaa acaacctctt caacctcggc atcgagggcc cggcacgcgc
     6001 ggccatggag tcgctcggcc tgaacctcga cgagctgatc gagcacgaag aggagcccgg
     6061 actcgggaac gggggcctgg ggcgcctcgc ggcctgcttc atggactcgc tcgcgacgct
     6121 cgacttcccg gccatcggcc acggcatccg ctacgagttc ggcatcttcg accaggagat
     6181 cgtcgacggc tggcagcgcg agcgcacgga ccgctggctc cgtaccggca atccctggga
     6241 ggtgcgccgg tacgagatcg agcaccccgt cggtttcggc gggtacaccc agacggtcgt
     6301 ggacggctcc ggccgctcgc gcgtgaaatg gacgcccgcc cggctcgtgc gcggcgtccc
     6361 ctacgacacg cccgtcgtgg gatacggcac ggtgacgacc aatttcctgc gcctctgggc
     6421 ggccgtcgcg ccggaggagt tcgacctcga cgccttccag gtcggggagt actggcgcgc
     6481 ggtcgacgag aagatccgca gcgagaacat cacgaaggtc ctctacccga acgacgcctc
     6541 cactgccggc aaggagctgc gcctccagca ggagtacttc ttcgtctcgt gcgccctcca
     6601 ggactgcatc cgcctgctgc tccagcggac cacgatcgac aagttcgccg acaagttcgc
     6661 gatccagctc aacgacacgc acccggcgct cgccgtgccg gagctcatgc ggctcctgat
     6721 ggacgagcac ggcctcggct gggacgaggc gtgggacatc acggagcgct cgttcaacta
     6781 cacgaaccac acgctgctgc ccgaggcgct cgaaacctgg cccctcccgc tcctcggccg
     6841 cctgcttccc cgccacctcg agatcgtctt cgagatcaac cgtcgcttcc tggacgcggt
     6901 gcgggcgcgt ttccccggcg acgacgcgag gctctcgcgc ctgagcctca tcgacgagcg
     6961 gggtgagaag agcgtccgca tggcgcacct ggctgcggtc gcgagccggc gggtgaacgg
     7021 ggtcgcggag ctgcattcgc ggctcctgcg ggagaccgtc ctcgccgatt tcgccgcgct
     7081 ctggccggag cgcttcacga acgtgacgaa cggcgtcacg cctcggcgtt tcgtcggcct
     7141 ggcgaacccg cgtctctcgt cgctcgttac cgaggcgatc ggcgacggct ggctccggga
     7201 cctcgaacgc ctctcgggcc tcgaaacgct cgcctccgac gcggtgttcc gcgagcgctg
     7261 gcgcgccgtg aagcaggcca acaaggagcg tctcgccggg tggctccgcg agacggtggg
     7321 ctgcgccgtc gacccgcaca cgctcttcga cgcgcagtgc aagcgcattc acgagtacaa
     7381 gcggcagcag ctcaacctgc tgcacgtcgt ctggctctac cagcggattc tgcgcggcga
     7441 cgtcgcgggc atgacgccgc ggacgtttct cttcgcgggg aaagcggcgc ccggctacca
     7501 cgccgcgaag ctcatcatcc ggctcgcgtc cgccgtcggg gagaccctcg agcggcaccc
     7561 cgtcgcgaag gacctcctct ccgtcgtgtt cgtccccgac ttcaacgtga agaacgcgca
     7621 gcggatctac ccggcggtcg acctctccga gcagatttcc accgcgggga aggaagcatc
     7681 ggggacggga aacatgaagt tcgcgatgaa cggcgcactc acgatcggca cgctggacgg
     7741 cgcgaacgtc gaaatccggg acgcggccgg cgcggagaac ttctttctct tcgggctcac
     7801 cgcggccggc gtggaggacg tcctgcgccg cggctacaag ccgcgcgacg tcatcgagtc
     7861 ggaccgggag ctcgccgacg tcctctggct cgtcgaatcc ggccatctcg tgcccgacga
     7921 gccggacctc ttccggccgt tcgtccgcac gctcgtggag gaggacccct atctcgtcct
     7981 cgcggatttc cgcgcctacg tcgagtgtca ggcacgcgtc tcggcggcct ggagcgacgt
     8041 cgtccggtgg acgagcgcgt cgatcctcaa cgtcgcgagg atgggccgct tcagctcgga
     8101 ccggtcgatc cgcgactacg tccgcgaggt ctggcacgtg cggcccgtgt cgctcgcgcc
     8161 ggtccgctga tcgcggcgcc gagatcggcg ggttgggccg cgcccgagtc tggtacggtt
     8221 gagcgatgcc cgagagccag cagcccctct cgctgcgtct cctgtccctg tacccgaaga
     8281 aatccgggtc cctcgcggtc ggcgcgctcg cccgcctcgg cctgccgaag cgcctgcgcc
     8341 ggccgctgct cgggcgcttc gcagccgcct acggcgcaaa cctcgacgag gccggccttc
     8401 cgctggagga gtacgcgagc ttcctcgact tcttcacccg ccggctcaaa ccgggcctgc
     8461 gcccgcagga accagccgtg ccgggcggaa tcaactcgcc cgtcgacggg gcgctcatcg
     8521 cgtccggccg cgtgacggaa gggacgctcc tgcaggcgaa gggcctgccg taccggctcg
     8581 acgagctcct cgacggcgac cctctcgcgt cggagctggc gggcgcgcat tacctcacgc
     8641 tctacctcgc gccgaaggac taccaccgca tccacgtccc gctcacgggc gcctgcgtct
     8701 cggtcggccg cgtcgagggc gagctctggc ccgtcaacga ggcgtcgacg cgcttcacgc
     8761 cgcgcctcta cgtgcggaat cgccgcgcgt actggatcgc gcgcggtgcg ggccccgacg
     8821 agggcctcgt cgccgcggcg gtcctcgtgg ccgcgacgca cgtcggtggc gtcgtgatcg
     8881 acccgcgctg gctctcgggc cggacgctcc cgcgccgcgg acgcctcgac gtcgcggccc
     8941 tgccggccgc
//
LOCUS       DAOMFY010000150         8869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2408_length_8869_cov_8.267983, whole genome shotgun sequence.
ACCESSION   DAOMFY010000150 DAOMFY010000000
VERSION     DAOMFY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2408_length_8869_cov_8.267983"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..2353
                     /gene="hypF"
                     /locus_tag="PLB01_12265"
     CDS             17..2353
                     /gene="hypF"
                     /locus_tag="PLB01_12265"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HQQ78119.1"
                     /translation="MRRRVRLEVRGAVQGVGFRPFVWRLAHALSLAGWVRNDVAGVFI
                     ELEGEGEALDAFVRALEEAPPPLARIRGIERSPAVPTGEKGFRILPSRGDGARTALVL
                     PDVTTCDACLAEVLDPRDRRHLYPFANCTNCGPRFTIVTELPYDRPNTTMASFPLCPE
                     CRAEYDDPRDRRFHAQPIACPVCGPSLEAWSPSGEVLARRGEALLATAEALRRGETAA
                     VKGLGGFHLVCDARSADAVAALRRRKQREEKPLALMVADVAMARALAEVSPEAEALLS
                     SPEAPIVLLPRRPDAAVASNVAPGRPELGLMLPATPLHHLLMRELGFPVVATSGNRSE
                     EPICTDEKEALTRLGEMADVFLVHDRPIARHVDDSVARVIAGAPRLLRRARGWAPLPV
                     PVRTPLPVILGVGAHMKTAVALSVGREVFLSQHVGDLETPEALDAFARVVADFERLYG
                     ATPVAVAHDLHPGYASTAFALRLGAERGIPALAVQHHHAHLASVLAENEIDTPALGVT
                     WDGTGYGTDGTIWGGEFLLGDAAGFARVAHLRPFRLPGGDAAVREPRRAALALLLEIL
                     GEKALDREDLPCVRSFSRAERDVLAQMLATGVNAPVTTSAGRLFDAVASLLGISQKSS
                     FEGQSAMALEAAAIEGFALTDGGRDVSDPLRLVVDSDGSAVLDWEPLIFTLLGKSSLG
                     APPSLLAAQFHAALADGILAAARHAEVPRVALTGGCFQNRLLTELSTARLRSAGFEPL
                     LHGSVPPNDGGIGLGQVLVAAERLKHLHRGTESRSGLG"
     gene            2372..2644
                     /locus_tag="PLB01_12270"
     CDS             2372..2644
                     /locus_tag="PLB01_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HQQ78120.1"
                     /translation="MCLGVPGKVVSIEASIIDMPMGVVSFGGITKEVCFAYTPDVKPG
                     EYVLVHVGFAISKLDEEQALEVFRTLQAMGELGDLEIPQPSGGGTP"
     gene            2641..3726
                     /gene="hypD"
                     /locus_tag="PLB01_12275"
     CDS             2641..3726
                     /gene="hypD"
                     /locus_tag="PLB01_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HQQ78121.1"
                     /translation="MKFVDEYRREEDAQAFVRAIRAKVTRPWTLMEICGGQTHTLLRS
                     GIDRMLPPEITLVHGPGCPVCVTPLTQIDRALAIAQRPGVVFTSFGDMLRVPGSTTDL
                     LTVKAKGGDVRMVYSPLDAVAIAQSSPDREVVFFAVGFETTAPANAMAVKRAKQLGLT
                     NFSVLASHVLVPPAMEAILSSPTNRVQGFLLAGHVCTVMGWTEYEPLGAKYRIPMVVT
                     GFEPLDLLQGIHMAVDALESGRHGVENQYVRTVSREGNPAAQAVIREVFEPCDRAWRG
                     IGVIPRSGYRLRPEYAPFDATLRFDVGAITADEPKECIAGEIMQGLKRPNECSAFGTR
                     CTPEHPLGAPMVSSEGACAAYWLYARA"
     gene            3726..4820
                     /gene="hypE"
                     /locus_tag="PLB01_12280"
     CDS             3726..4820
                     /gene="hypE"
                     /locus_tag="PLB01_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019748090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HQQ78122.1"
                     /translation="MAEAKPPSPAPDLGAAIAAASCPVPVASAARTILMAHGGGGRLT
                     QRLIESLFLPSFDNPALRELHDGAILETGGVRLAFSTDSFVIHPVFFPGGDIGSLAVH
                     GTVNDLAMCGAQPLALSTALILEEGFAIADLERIVASMQRAASGAGASIVTGDTKVVD
                     KGKGDGVFVNVTGVGIVPEGIVIAPRRARPGDVVLVSGEIAVHGIAIMSLREGLRFGT
                     TLESDTAALNGLVANLLAAAGESVHVLRDPTRGGVASTLNEIAGQAGVGIRLEETALP
                     VSEEVRGACEILGLDPLYVANEGKFLAIVAREAADGALAALRAHPLGGKAAAIGTVVA
                     EAPGRVTLKSRIGGVRVVDLLTGEQLPRIC"
     gene            4822..5208
                     /locus_tag="PLB01_12285"
     CDS             4822..5208
                     /locus_tag="PLB01_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HQQ78123.1"
                     /translation="MHEMGVAAEVQRIARASVADRGPGRIACVRLAVGDLAAVEPELL
                     TYAWAALVAGTPEEGATLEIDWRPATQTCLVCGDLPGRAAGSWLRVCPRCGGVLTVTG
                     GDDLDVLQVVFEEEDPVEREARGGAR"
     gene            5205..5900
                     /gene="hypB"
                     /locus_tag="PLB01_12290"
     CDS             5205..5900
                     /gene="hypB"
                     /locus_tag="PLB01_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HQQ78124.1"
                     /translation="MTEIDVKQKVLAANAAAAADLRERFGRSGTLVVNLISSPGSGKT
                     TLLERTIAALKGSLRLGGLEGDIATERDAERLRRTGIPARQILTGGSCHLDARQIDRA
                     LAEEGASLANLDILFIENVGNLICPTGYDLGEDFKVVLLSVTEGEDKPFKYPAIFSKA
                     AVTVVTKIDLLPYVPFDLPAVREQVATLAPEGRLVEVSATTGAGMDAWLSLLTDALRA
                     KRAAVYAGEGVGS"
     gene            5902..6552
                     /locus_tag="PLB01_12295"
     CDS             5902..6552
                     /locus_tag="PLB01_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78125.1"
                     /translation="MAGGLFLTLAGAAAGVGALHTLAPDHWVPFAALARAQRWSAAKT
                     ARITVLCGFGHVTVSALLGLLGLVFGRAIFEKAGARMEAVAGILLVGFGLAYGVWGMR
                     RAAGRRVHGHAHVHYDHVHDASKTTAWSLFLLFSADPCVAVIPLLFAAAPLGTAPTVG
                     IVLVYETATIATMIALVLPARAGFERLRLPWLDHWGDAVAGGVIAATGLVVMALGI"
     gene            6655..8043
                     /gene="creD"
                     /locus_tag="PLB01_12300"
     CDS             6655..8043
                     /gene="creD"
                     /locus_tag="PLB01_12300"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HQQ78126.1"
                     /translation="MRDSLLARAAVILLVFAVLLIPLGMIRGLVAEREARRREAVAEI
                     AQAWGGPQTVLGPVLVVPHRVVRKEVVVPPPAPGMPASTVVTPVEKTRVETAYAAFLP
                     DRLAFEGRLVPERRKRGLFEAVVYAVELKATGTFPALDLHALGIDPDAADFAGAFLSV
                     AVADTRGIREEASVLWDGANERFRPGPGASGLGDAGLNAPLGSLDGGKAHAFSFTLRL
                     RGTESLSVAPLGASTEATFSSHWPSPSFTGAFPPETRTVGASGFTAAWRVSSFGRPYA
                     QTWRACPPKEQIAASSFGTRLYLPADEYQQTTRALKYAVLFVGLTFLAFALFEILGHA
                     RLHPLQYLFVGFALALFYLLLLSLAEHAGFGPAYLAAASATTLLVAVYSVWALAGARR
                     GALLGAALASLYGTLYVLLRLEDWALLLGAGVLFAILAAVMFGTRRVDWWALSSAPPR
                     RFPPPAAPPVRA"
     gene            8159..8632
                     /locus_tag="PLB01_12305"
     CDS             8159..8632
                     /locus_tag="PLB01_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQQ78127.1"
                     /translation="MLTRWNDPLKDIKTLQNQVTRMLGDFPAGLRPDDMPTANWAPPA
                     DVVETDDRLSFSFEVPGFRQDELTLHVENGILSLEGDRKFEEETKDKNYHRIERAYGH
                     FLRSFALPMNVDTGKVSASLADGVLKVDIPKREEAKPKAIPIGTGSPKPLAAGKK"
BASE COUNT         1166 a         3306 c         3041 g         1356 t
ORIGIN      
        1 ccttagagtc gttgccgtgc gcaggcgcgt gaggctcgag gtccgcgggg cggtgcaggg
       61 cgtcggattc cggccgttcg tctggcgcct cgcgcacgcg ctttctctcg cgggctgggt
      121 gcggaacgac gtcgcgggag tcttcatcga gctggaggga gagggcgagg cgctcgacgc
      181 cttcgtccgc gcgctcgaag aggcgccccc tcctctcgcg cgcatccggg ggatcgagcg
      241 gtcgccggcg gtcccgacgg gagaaaaggg attccggatc cttccgagcc gcggcgacgg
      301 agcgcgcacg gccctcgtcc tgcccgacgt caccacgtgc gacgcgtgcc tcgccgaggt
      361 gctcgatccg agagaccggc ggcacctcta cccgttcgcg aactgcacga actgtgggcc
      421 gcgcttcacg atcgtgacgg agcttcccta cgaccggccc aacacgacga tggcgtcgtt
      481 cccgctctgc cccgagtgcc gggcggagta cgacgacccg cgcgaccggc ggttccacgc
      541 gcagccgatc gcctgccccg tgtgcggccc gtctctcgag gcctggtcgc cgtcgggcga
      601 ggtcctcgcg cggcgcggag aggcgctcct cgcaaccgcc gaggcgctcc ggcgcggcga
      661 aacggccgcc gtgaagggcc tcggagggtt ccacctcgtg tgcgacgcgc gcagcgcgga
      721 cgcggtggcc gccctccggc ggcggaaaca gagagaggaa aagccgctcg ccctgatggt
      781 ggcggacgtc gcgatggcgc gcgcgctcgc cgaggtgtcc ccggaagccg aggctctcct
      841 ctcgtctccg gaggcgccga tcgtcctcct tccccgccgt cccgacgccg ccgtcgcgtc
      901 gaacgtcgcc cccggccggc cggagctggg cctcatgctc ccggcgacgc ccctccacca
      961 cctcctgatg cgcgagctcg gctttcccgt cgtcgcgacg agcggcaacc gcagcgagga
     1021 gcccatctgc acggacgaga aggaagccct cacgcgcctc ggggaaatgg ccgacgtctt
     1081 cctcgtgcac gaccggccga tcgcgcggca cgtggacgac agcgtcgcgc gcgtcatcgc
     1141 gggcgctccc cgtctcctga ggcgcgcgcg cggctgggct cccctgcccg tgcccgtccg
     1201 cacgccgctc cccgtgatcc tcggcgtggg cgcgcacatg aagacggccg tggcgctctc
     1261 ggtcggacgc gaggtcttcc tctcccagca cgtcggcgac ctcgagacac ccgaagcgct
     1321 ggacgccttc gcgcgcgtcg tcgccgactt cgagcggctc tacggagcga cgccggtcgc
     1381 cgtcgcgcac gatctccatc ccggctacgc gtccacggcc ttcgcgctga ggctcggcgc
     1441 cgagcgcggc atcccggccc tcgcggtcca gcatcaccac gcgcacctcg cgtccgtcct
     1501 cgccgagaac gagatcgaca ctcccgcact cggcgtcacg tgggacggca cgggatacgg
     1561 gacggacggc acgatctggg gcggcgagtt cctcctcggc gacgcggcgg gcttcgcccg
     1621 cgtcgcgcac ctccgcccgt tccgactccc cggcggcgac gccgccgtgc gggagccgcg
     1681 gagggcggcg ctcgcgctcc tgctcgagat cctcggcgag aaggcgctcg atcgcgagga
     1741 cctcccgtgc gtgcggtcct tctcgcgcgc cgagcgcgac gtcctcgcgc agatgctcgc
     1801 cacgggcgtc aacgctccgg tgacgacgag cgccggccgt ctcttcgatg cggtcgcgtc
     1861 gctcctcgga atctcccaga agagttcctt cgaaggtcaa tcggccatgg cgctcgaagc
     1921 agccgcgatc gaagggtttg cgctcaccga cggcggccgc gacgtctcgg accctctccg
     1981 cctcgtcgtc gactcggatg gatcggccgt actcgactgg gaaccgctga tcttcaccct
     2041 tctaggaaaa tcttctctgg gtgctcctcc ttcgctcctc gccgcgcagt tccacgccgc
     2101 tctcgcggac gggatcctcg cggcggcgcg gcacgccgag gtcccccgcg tcgcccttac
     2161 ggggggctgc ttccagaaca ggctcctgac ggagctttcg accgcccggc tgcgttcggc
     2221 gggcttcgag cccctcctgc acggctcggt cccgccgaac gacggtggaa tcggcctcgg
     2281 ccaggtcctc gtcgccgcgg agaggctgaa gcatctgcat cgggggactg agtcgcggtc
     2341 cgggctaggc tgaacgaaaa tggggtgcac gatgtgtctc ggagtaccgg gcaaggtcgt
     2401 cagcatcgag gcgagcatca tcgacatgcc gatgggcgtc gtctcgttcg ggggcatcac
     2461 gaaagaggtg tgcttcgcgt acacgccgga cgtgaagccg ggcgagtacg tcctcgtcca
     2521 cgtcggcttc gcgatcagca agctcgacga ggagcaggcc ctcgaggtct tccggacgct
     2581 ccaggcgatg ggcgaactcg gcgacctcga gatcccgcag ccgtccggag gcgggacacc
     2641 gtgaagttcg tcgacgagta ccgccgcgag gaggacgcgc aggccttcgt gcgcgcgatc
     2701 cgcgcgaagg tcactcgtcc gtggacactc atggaaatct gcggcgggca gacgcacacg
     2761 ctcctgcgct ccgggatcga ccggatgctg ccccccgaga tcacgctcgt gcacgggccg
     2821 ggctgtcccg tctgcgtcac gccgctgacg cagatcgacc gggcgctcgc gatcgcgcag
     2881 cgccccggcg tcgtcttcac gtccttcggg gacatgctcc gcgtcccggg ctcgacgacg
     2941 gacctcctga ccgtcaaggc gaaaggcggc gacgtgcgga tggtctactc gccgctcgac
     3001 gccgtcgcga tcgcgcagag cagcccggac cgggaagtcg ttttcttcgc cgtcgggttc
     3061 gagacgacgg ctcccgcgaa cgcgatggcc gtcaaacgcg cgaagcagct cggactgacg
     3121 aacttctcgg tcctcgcctc gcacgtcctc gtgccccccg cgatggaggc gatcctctcc
     3181 tcgccgacga accgggtgca gggcttcctc ctcgcggggc acgtctgcac cgtcatgggc
     3241 tggacggagt acgagcccct cggagcgaag taccgcatcc cgatggtcgt cacgggcttc
     3301 gagcccctcg acctcctgca ggggatccac atggcggtcg acgcgctcga gagcgggcgc
     3361 cacggcgtcg agaaccagta cgtgcgcacg gtctcgcgcg aagggaaccc cgccgcgcag
     3421 gcggtcattc gcgaggtctt cgagccctgc gaccgcgcct ggcgcgggat cggcgtgatc
     3481 ccgcggagcg gctaccgcct gcgccccgaa tacgcgccgt tcgatgcgac cctgaggttc
     3541 gacgtcggcg cgatcaccgc cgacgagccg aaggagtgca tcgcgggcga gatcatgcag
     3601 ggcctcaagc gcccgaacga gtgctcggcc ttcgggacgc gctgcacgcc cgagcacccc
     3661 ctcggcgcgc cgatggtgtc gtccgaggga gcctgcgccg cctactggct ctacgcgagg
     3721 gcctgatggc cgaagcgaag ccgccctctc ccgcccctga tctcggcgcc gccatcgcgg
     3781 cggcctcgtg ccccgtgccc gtggcctccg ccgcacggac gatcctcatg gcgcacggcg
     3841 gcggcggccg cctcacgcag cgcctcatcg agagcctctt cctcccgagc ttcgacaacc
     3901 cggcgctccg cgagctgcac gacggcgcga tcctcgagac cggcggcgtc cgcctcgcct
     3961 tctcgacgga ctcgttcgtg atccaccccg tcttcttccc cggcggcgac atcggctcgc
     4021 tcgccgtgca cggtaccgtg aacgacctcg cgatgtgcgg cgctcagccg ctcgcacttt
     4081 cgaccgcgct catcctcgaa gagggattcg cgatcgcgga cctcgagcgg atcgtcgcgt
     4141 cgatgcagcg cgcggcctcc ggcgcgggcg cgtcgatcgt cacgggggac acgaaggtcg
     4201 tcgacaaggg caagggcgac ggggtcttcg tgaacgtgac gggcgtcggg atcgtccccg
     4261 agggaatcgt catcgcgccc cgccgcgcgc gcccgggcga cgtcgtcctc gtgagcggcg
     4321 agatcgccgt ccacgggatc gcgatcatgt cgctccgcga gggcctgcgc ttcgggacga
     4381 ccctcgagag cgacacggcc gccctgaacg gcctcgtcgc gaacctcctc gcggccgcgg
     4441 gcgagtccgt ccacgtcctg cgcgacccga cgcggggcgg cgtcgcctcg accctcaacg
     4501 agatcgcggg ccaggccgga gtcggaatcc gcctcgagga gacggccctt ccggtctccg
     4561 aggaagtgcg cggggcctgc gagatcctcg ggctcgaccc gctctacgtc gcgaacgagg
     4621 gcaagttcct cgcgatcgtc gcgcgggagg cggccgacgg ggccctcgcc gcgctgcgcg
     4681 cgcacccgct cggcgggaag gccgccgcga tcgggacggt cgtcgcggag gcgccgggcc
     4741 gcgtcacgct gaagagccgc atcggcggcg tccgcgtcgt ggacctcctc accggcgagc
     4801 agcttccgag gatctgctga gatgcacgag atgggcgtcg ccgccgaagt gcagcgcatc
     4861 gcgcgcgcgt ccgtcgcgga ccgcggcccc gggcggatcg cgtgcgtgcg cctcgccgtc
     4921 ggggacctcg cggccgtcga gcccgagctc ctgacgtatg cgtgggcggc tctcgtcgcg
     4981 ggcacgcccg aggagggagc gaccctcgaa atcgactggc ggcccgccac gcagacgtgc
     5041 ctcgtctgcg gcgacctgcc gggccgcgcc gcgggctcgt ggctcagggt ctgtccgcgc
     5101 tgcggcggcg tcctcaccgt gacgggcggc gacgatctcg acgtccttca ggtcgtcttc
     5161 gaggaggaag atcccgtgga acgcgaagct cgcggagggg cgcgatgacc gaaatcgacg
     5221 tgaagcagaa agtgcttgct gcgaacgcgg cggccgcggc cgacctccgc gagcgcttcg
     5281 gacgctccgg cacgctcgtc gtgaacctca tttcctcgcc gggctccggc aagacgacgc
     5341 ttctcgagag gacgatcgcg gccctcaagg ggtcgctgcg gctcggcggc ctcgagggcg
     5401 acatcgcgac cgagcgcgac gccgaacgcc tgcggaggac cgggatcccg gctcgccaga
     5461 tcctgacggg gggctcctgc catctcgacg cgcgccagat cgaccgcgcg ctcgccgagg
     5521 agggcgcctc cctcgcgaac ctcgacattc tcttcatcga gaacgtcggg aacctcatct
     5581 gcccgacggg ctacgacctg ggcgaggact tcaaggtcgt cctcctgtcg gtcaccgagg
     5641 gggaggacaa gccgttcaag tacccggcga tcttctcgaa ggcggccgtc acggtcgtca
     5701 cgaagatcga cctcctcccc tacgtcccgt tcgacctgcc cgccgtgcgc gagcaggtcg
     5761 cgacgctcgc gcccgagggg cgcctcgtcg aggtctccgc gacgacgggc gccgggatgg
     5821 acgcgtggct ctcgctcctg acggacgcgc tccgggcgaa gcgcgcggcc gtctacgccg
     5881 gcgagggcgt cgggagctga cgtggcgggc ggtctcttcc tcacgctggc cggagcggcc
     5941 gccggcgtcg gcgccctcca cacgctcgcg cccgaccact gggtcccctt cgccgcgctc
     6001 gcgcgcgcgc aacgatggtc cgccgcgaag accgcccgca tcacggtgct ctgcggcttc
     6061 ggccacgtga cggtctcggc cctcctcggc ctcctcgggc tcgtcttcgg ccgcgcgatc
     6121 ttcgagaagg ccggcgcgcg catggaggcg gtcgcgggga ttctcctcgt gggcttcggc
     6181 ctcgcgtacg gcgtctgggg gatgcgccgc gccgcgggcc gccgcgtgca cggccacgcg
     6241 cacgtccact acgaccacgt ccacgacgcg tcgaagacga cggcgtggtc gctctttctt
     6301 ctcttctccg cggacccctg cgtcgccgtg atcccgctcc tcttcgcggc ggcgccgctc
     6361 ggcaccgcgc ccacggtcgg aatcgtcctc gtctacgaga ccgccaccat cgcgacgatg
     6421 atcgccctcg tgctcccggc ccgtgcggga ttcgagcgcc tgcgcctccc gtggctcgac
     6481 cactggggcg acgccgtcgc gggcggcgtg atcgcggcga cgggcctcgt cgtgatggcc
     6541 ctcgggatct gacggcaccc tctccgccgt gtgacgcctt ctccgaaacg gagcgagacc
     6601 ggccgcgcgc cgcctggatt tgcgtgggca tgtactttgc gatacaaagt aaacatgaga
     6661 gattcgctcc tcgctcgtgc cgccgtcatc cttctcgtct tcgcggtcct cctcattccg
     6721 ctcggcatga tccgcggcct cgtcgccgag cgcgaagcgc ggcgccgcga ggccgtcgcc
     6781 gagatcgcgc aggcctgggg cggcccccag acggtcctcg ggcccgtcct cgtggtcccc
     6841 caccgggtcg tccgcaagga ggtcgtcgtc ccgcccccgg cgcccggaat gccggcatcc
     6901 accgtcgtga cccccgtcga gaagacgcgc gtcgagacgg cgtacgcggc gttcctgccc
     6961 gaccgcctcg cgttcgaggg ccgcctcgtt ccggagcgca ggaagcgcgg gctcttcgag
     7021 gccgtcgtct acgccgtcga gctgaaggcc acggggacgt ttcccgctct ggacctccac
     7081 gccctcggga tcgacccgga cgccgcggac ttcgcggggg cgttcctgtc ggtcgccgtc
     7141 gcggacacgc gcgggatccg cgaggaggcg tcggtcctct gggacggcgc gaacgagcgc
     7201 ttccggcccg gcccgggcgc gtcgggcctc ggggatgccg ggctgaacgc gcctctcggg
     7261 tccctcgacg gcgggaaggc gcacgcgttc tcgttcacat tgagacttcg cgggacggaa
     7321 tccctttcgg ttgccccgct cggcgcctcg accgaagcga cgttctcctc gcactggccc
     7381 tcgccgagct tcacgggcgc tttcccgccc gagacgcgca cggttggcgc gtccggcttc
     7441 acggcggcgt ggcgcgtttc gtcgttcggg cgcccgtacg cgcagacgtg gagggcctgc
     7501 ccgccgaagg agcagatcgc ggcatcctcg ttcggcacgc gcctctacct cccggccgac
     7561 gagtaccagc agacgacgcg cgcgctgaag tacgcggtcc tcttcgtcgg cctcacgttc
     7621 ctcgcgttcg cgctcttcga gatcctcggg cacgcgcgcc tccatccgct gcagtacctc
     7681 ttcgtcggct ttgcgctcgc cctcttctac ctgctgctgc tctccctcgc cgagcacgcc
     7741 ggcttcggcc cggcctacct cgcggccgcg tccgcgacga cgctcctcgt tgcggtctat
     7801 tccgtgtggg cgctcgcggg cgcgaggcgc ggcgcgctcc tcggcgcggc tctcgcgtcg
     7861 ctctacggga cgctctacgt cctcctgagg ctcgaggact gggcgctcct cctcggggcg
     7921 ggtgtcctct tcgcgatcct cgcggccgtc atgttcggca cccggcgcgt ggactggtgg
     7981 gcgctctcgt ccgcgccccc tcgccgcttc ccgccgccgg cggcgcctcc cgtgagggcc
     8041 tgatgcgtct gcatcacttg acgcgggcga tcgccgttcc catgggttct agtggaacgg
     8101 tcgacgcgcc cgggtttcgt tccctccgtt cgcgcacggc agaaacaggg aggcttgcat
     8161 gctgacgcgc tggaacgacc cgctcaagga catcaagacg ctgcagaacc aggtgacgcg
     8221 gatgctcggg gatttccccg ccggcctccg cccggacgac atgccgacgg ccaactgggc
     8281 gccgccggcc gacgtcgtcg agaccgacga ccggctgagc ttctccttcg aggtacccgg
     8341 attccgccag gacgaactga ccctccacgt cgagaacgga atcctgtccc tcgagggaga
     8401 tcgaaaattc gaggaggaaa cgaaggacaa gaactatcac cggatcgagc gtgcttacgg
     8461 gcacttcctc cgctcgttcg cgcttccgat gaacgtcgac acgggcaagg tctcggcctc
     8521 tctggccgac ggcgtgctca aggtcgacat tcccaagcgg gaagaggcga agccgaaggc
     8581 cattcccatc gggaccggct cgcccaagcc cctcgcggca gggaagaaat aacgcgaggg
     8641 ttcccggaac cgcgctccgg cgccaggccg gagcgattgg cgggcggcgc gtgaccgggc
     8701 gcgccgcccg catcgtcttc ggccgcgata tcctgagacg gtgtctcccg cccttcccca
     8761 cttcgacgcg gtccccctcc tccagtgcct caagcccgcg gagcgccgcc gcctcgcgcc
     8821 catgtgccgc gccgtcgcct acgagaaagg cggggaggtc ttccgcgag
//
LOCUS       DAOMFY010000151         8789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2443_length_8789_cov_6.865239, whole genome shotgun sequence.
ACCESSION   DAOMFY010000151 DAOMFY010000000
VERSION     DAOMFY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2443_length_8789_cov_6.865239"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="pal"
                     /locus_tag="PLB01_12310"
     CDS             <1..368
                     /gene="pal"
                     /locus_tag="PLB01_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQQ78128.1"
                     /translation="PPVVVEEPVDKYVQDIFFDYDKSEIRPDSREKLEMAAGWMKKHP
                     DAEFLLEGHCDERGTREYNLALGDRRANATKDYLASLGVDPAKLKTISYGKERPFVEG
                     HDESAWSKNRRTHFVLTKK"
     gene            539..1099
                     /gene="efp"
                     /locus_tag="PLB01_12315"
     CDS             539..1099
                     /gene="efp"
                     /locus_tag="PLB01_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQQ78129.1"
                     /translation="MISATEIRPGMIIIHNGELHRAHSVLHKTPGNLRGFVQAKLRNL
                     KSGAMNEHRFRSEDKVEKASLDTHQMQYLYSDSEGHHFMNQETFDQIRLDDEVVGESM
                     KYLLPETVIEIDFYDGNPVSVELPTTVNLKIVEVEPGMKGATASASYKPAKLETGLMV
                     QVPQFVEAGTVIKIDTRDNSYLERVS"
     gene            1177..1986
                     /locus_tag="PLB01_12320"
     CDS             1177..1986
                     /locus_tag="PLB01_12320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQQ78130.1"
                     /translation="MLVAVAGHDASGPAVAGAGAAPSRACPVRDPETGACFPSADAAD
                     LAWLKRPVDPAAVRAEDEEDPDEGAEDEDAGPAPAAAEKLSPAQLAFWRDLARTVTGL
                     VVRKKNSRPASLPEEEIASLLASDFPIPLEAFDRAKFRDTFFAKRGRQKKHYAIDLPA
                     PRGTPVLAVTDGVIERLGRDRKGGKVVYLRDTTGRYTFFYAHLAKHEKGLRPGDRVVK
                     GQRLGDVGATGHVIGGPHLHFAIFRDEDDVTGGRALVVNPYLVFSPLLAPR"
     gene            2036..4882
                     /locus_tag="PLB01_12325"
     CDS             2036..4882
                     /locus_tag="PLB01_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQQ78131.1"
                     /translation="MAEEIRRDAEGGVRDRFLSEFALALVEGVQLQEFLDWSISQIGR
                     ILEVDRLTLFLFGGEAGPATLVARAAWATGGAPPLASLVPPGAAPRIPPSLRRFQPVV
                     APDVLAEPELIDQVDACRRNGIVSLLLVPIGIDGVLRGFVGAATVERRDWSPDDVAFL
                     ESAVHHLSAALKQTELVEELGRERDRLSVLFDLASAVQRSTTVKDVVETALAGLRDTL
                     LYPIAVFSLLTPEGDALTGLGGYGGSRDGDFVDRIPLAPELPTYSFRVLESGQPMIIA
                     DVRLEPESASRERFVRIGARSVGIFPMRSAGQTIGVMAVATRDESRHIEFDDVETLQS
                     LADFVGVALEQRRSAEAVAQSMREARSLADASRALLTRTANREVLLTQILDALATHYG
                     RDASSMLLVDRDRRALVQFGRRGKWWSPGDPVSVIPLDAPGLIPLAARTARVVNVPDV
                     SRAPDYVVGWPEARSELVVPLVLDEKVIGVFDLQSGEPAAFSDADARNIAAFAERAAL
                     ALRLAELVGAFEQRTRVLEAVARATQLLNFRLHAPDVLTSVVEETTRAFPAADGAVAW
                     VADPPGRNLSVAAVFGVGVMTLHGGGSGPRPVGRLRSAGRAFRENRPVFTAVNGLDEL
                     AEGDPVESRAKARAAVADADVRALMAVPIRVGDQRLGVLEVMSYRTGAFTREDGATLT
                     LLAEQCAIALRNARLIEELQRSNRLKDDFLANLSHEVRTPLTGIVGWAEVLLDSHGGD
                     PPVKRALEAILGQSDTLSRMLGDLIDLSRIDNFGLELRRERVRLSETIAGALDAVAPG
                     AAKRGVAIRCHIEPDLPAMEGDPARLKQVVWNLLANGVKFSPTGGTVHVTVARTGDGG
                     IELSVEDEGHGIDPEFLPHVFERFRQEETSSSRRFGGLGVGLSIARAIVEAHGGTIAA
                     ESEGRERGSRFRVGFPPERVVGPVVRGTVHGSGR"
     gene            4879..6432
                     /locus_tag="PLB01_12330"
     CDS             4879..6432
                     /locus_tag="PLB01_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQQ78132.1"
                     /translation="MSPDPRSLEERLRQAEERIAALNRIGIALSAERDLEKLLETILT
                     ESRRFSGSEAGSLYLAEDAAGGGRRLRFKLAQNDAVSFAFKERTVPVDEGSLAGWVAV
                     HGVPLVLEDAYAIPKDAPYRHNDAFDVATGFRTRAMLVVPMKDHLGDLVGVLQLMNRL
                     GPAGPEPYPEDLVPLLLSLATQAAVCLKANQLTASIRRLFEDFAQAAIVAVEQRDPTT
                     AGHSNRVAALTVELAGLVDRAPDGPYAAVSFSREEMREIFTAALLHDFGKISIPERVL
                     VKAKKIEEDGLRRIRDRFDYVLAASESEDARALLERLAKDGRPVTDRDLAALAEAADA
                     RSREFEAAFEEVAAANEPTILPKDARGALERLVARTWRDRRGRTATLLLPEEFRFLSI
                     AQGSLSQEERLDVESHVTHTWRFLSTIPWTPDLARVPQIAYAHHEKLDGSGYPRRVAS
                     PEIPPAARALTVCDIYDALTASDRPYKKAMPHDRALEVLAAEAEKDKLDTWLVKAFIQ
                     GRVWLALGR"
     gene            6429..7100
                     /locus_tag="PLB01_12335"
     CDS             6429..7100
                     /locus_tag="PLB01_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78133.1"
                     /translation="MRAALAAALLLGAPLAAAAPARWHFTFGKTWEISILFEREEKGD
                     STRLLVSAPAGRFVFLSSQDPSGRDSTESIRRLPDGETFTRRLVLSSFKEIPACSSVR
                     APDACVVFAGARGTFAAGFSEFAGDRAPAAREKASALVSPGMRDALFALAPILPAFLE
                     FGSYSKDFLALVWPERFSAPQKLVRGERKPGCDFDATFGFPCSEEEKAREARRFADAG
                     AATRP"
     gene            7156..7749
                     /locus_tag="PLB01_12340"
     CDS             7156..7749
                     /locus_tag="PLB01_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ78134.1"
                     /translation="MTGPNPTPPGDVTGLLLAWNGGDREALERLVPLVYEELRKRAQR
                     ALAKERRGHTLQPTALVHEAYMRLVDQNRVPWQNRFHFYAVSARAMRQVLVDHARRRL
                     ADKRDGGKTFVPLSRAGAEAATPPPSLDVLALDRALERLATLDPRQAKLVELRVYGGL
                     TIDEAAEALGVSAATVSREWRHAEAWLHREMAGSGAP"
     gene            7746..>8789
                     /locus_tag="PLB01_12345"
     CDS             7746..>8789
                     /locus_tag="PLB01_12345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ78135.1"
                     /translation="MTPELYARAKEIFLSAQEAAEEERGTLVAERCGGDEPLRREVEA
                     LLASDRAGAGFLDRAALPVPPWDEVEPAPPTRVGPYRVVGEIGRGGMGSVYLAERADD
                     AYAGRVALKLVRRGMDTEDILRRFRTERQILASLQHPNIARLLDGGTTEDGRPYFVME
                     YIEGRTLTEDARARALPLKDRLTLFRVVCAAVQHAHRNLVVHRDLKPSNVMVTADGAV
                     KLLDFGLAKVLGTEAPAPERTTGATAIFTPEYASPEQLAGRAITTSTDVYSLGVVLYE
                     LLADAHPYREDAASPARLWQAVCETDPPPPSAVAARTGGPAARRAPALAGDLDAIVLK
                     ARRKDPSRRYATVD"
BASE COUNT         1250 a         3209 c         3070 g         1260 t
ORIGIN      
        1 ccccgccggt cgtcgtcgag gagccggtcg acaagtacgt ccaggacatc ttcttcgact
       61 acgacaagtc ggagatccgg ccggactccc gcgagaaact cgaaatggcc gcgggctgga
      121 tgaagaagca ccccgatgcc gagttcctcc tcgagggcca ctgcgacgag cgcggcaccc
      181 gcgagtacaa cctggccctc ggcgaccgcc gcgcgaacgc gacgaaggat tacctcgcct
      241 cgctcggcgt cgacccggcg aagctcaaga cgatctccta cggcaaggag cggccgttcg
      301 tggaaggtca cgacgagtcg gcctggtcga agaaccgccg gacgcacttc gtcctgacga
      361 agaagtaagc gccctttttt caggtctttc cggcggcccg ggtcctcgga cccgggccgc
      421 ttgtctttcc gtcgaagggt gggttaggct tgaagaccgc cagcgggtcg ggccgggagg
      481 atcgtcagcc cccggtgtca ggagcgcccg cggtgattcc agaactcttg gagtaccaat
      541 gatttccgcc accgaaatcc gcccgggcat gatcatcatc cacaacggcg agctgcaccg
      601 cgcgcacagc gtcctgcaca agacgcccgg gaacctccgc ggattcgtcc aggcgaagct
      661 ccggaacctc aagtccggag ccatgaacga gcaccggttc cggtccgagg acaaggtcga
      721 gaaggcctcc ctcgatacgc accagatgca gtacctctac tcggactccg agggtcatca
      781 cttcatgaac caggagacct tcgaccagat ccgcctcgac gacgaggtcg tgggcgagtc
      841 catgaagtac ctgctccccg agaccgtgat cgagatcgac ttctacgacg gcaacccggt
      901 ctccgtggag cttcccacga cggtgaacct gaagatcgtc gaggtcgagc ccggcatgaa
      961 gggcgcgacg gcgtccgcgt cctacaagcc ggccaagctg gagacgggac tcatggtgca
     1021 ggtgccgcag ttcgtcgagg ccggaacggt catcaagatc gacacgcgcg acaactctta
     1081 cctggagcgg gtgagctgag tcttctcctc gcggccgccc tcgccggagc gctgggcgcg
     1141 gccggagagg tgtcggcggc cggctcggag ccccccgtgc tcgtcgccgt cgcgggtcac
     1201 gacgccagcg gtccggccgt cgccggagcg ggtgcggccc cgtcccgggc atgccccgtg
     1261 cgcgacccgg agacgggcgc gtgcttcccc tcggccgacg cggccgacct cgcgtggctg
     1321 aaacggccgg tcgatcccgc cgccgtgcgc gccgaggacg aggaggatcc ggacgaggga
     1381 gccgaggacg aggacgccgg tccggcgccc gcggccgccg agaagctctc gccggcgcag
     1441 ctcgcgttct ggagggacct cgcgcggacg gtcacgggcc tcgtcgtgag gaagaagaac
     1501 agccggcccg cgtcgctgcc cgaggaggag atcgcgtcgc tcctcgcatc ggattttccg
     1561 atcccgctcg aggcgttcga ccgtgcgaag ttccgggaca cgttcttcgc caagcgcggc
     1621 cgccagaaga aacactacgc gatcgacctg cccgctccgc gcgggacgcc ggtcctcgcc
     1681 gtgaccgacg gcgtcatcga gcgcctcggg cgcgaccgga agggcgggaa agtcgtgtac
     1741 ctgagggaca cgaccggccg ttacacgttc ttttacgcac acctcgcgaa gcacgagaag
     1801 gggctcagac cgggggaccg cgtcgtgaaa ggccagcggc tcggcgacgt cggcgcgacc
     1861 ggccacgtga tcggcgggcc tcacctgcac ttcgcgatct tccgcgacga ggacgacgtg
     1921 accggcggcc gggccctcgt cgtgaaccct tacctggtgt tcagccccct cctcgcgccc
     1981 cgctgaggag acgcgccggc ctccccggct ccgggcggcg tcataatccc ccgccgtggc
     2041 cgaggaaatc cgccgggacg ccgagggcgg cgtaagggat cgttttctct cggagttcgc
     2101 cctcgcgctc gtcgagggcg tccagcttca ggagttcctg gactggtcca tctcgcagat
     2161 cggccggatc ctcgaggtcg accgcctcac cctctttctg ttcgggggcg aggcgggacc
     2221 cgcgacgctc gtcgcgcgcg cggcctgggc caccggggga gcgcctccgc tcgcctccct
     2281 cgtgccgccc ggcgccgctc cgaggattcc cccgtcgctt cggcggttcc agccggtcgt
     2341 cgcgccggac gtgctcgcgg agcccgagct gatcgaccag gtggacgcgt gccggcggaa
     2401 cggaatcgtc tcgctgcttc tcgtcccgat cggaatcgac ggcgtcctcc gcggcttcgt
     2461 cggcgccgcg accgtcgagc ggcgggactg gtcgcccgac gacgtcgcgt tcctcgagtc
     2521 ggcggtccac cacctctcgg cagcgctcaa gcagaccgag ctcgtcgagg agctgggccg
     2581 cgagcgcgac cgactgtccg tgctgttcga cctcgcgtcg gcggtccagc gctcgacgac
     2641 cgtgaaggac gtcgtcgaga cggccctcgc gggcctccgc gacacgctcc tctatccgat
     2701 cgccgtcttc tccctgctca cgcccgaggg cgatgccctg accggactgg gcgggtacgg
     2761 cgggagccgg gacggagact tcgtggaccg gatcccgctc gcgcccgagc ttccgacgta
     2821 ctcgttccgg gtgctcgaga gcggccagcc gatgatcatc gcggacgtgc gcctcgagcc
     2881 ggagagcgcg tcgcgcgagc gcttcgtccg gatcggggcg cgctccgtgg ggattttccc
     2941 gatgcgctcc gcgggccaga ccatcggcgt catggcggtc gcgacacggg acgagtcgcg
     3001 acacatcgag ttcgacgacg tcgagactct ccagtcgctc gcggatttcg tgggcgtcgc
     3061 cctcgagcag cgccgctcgg ccgaggccgt cgcgcagtcc atgcgcgagg cgcgcagcct
     3121 cgcggacgcg tcgcgcgcgc tcctgacgcg cacggcgaac cgcgaggtcc tcctgacgca
     3181 gatcctcgat gcgctcgcga cccactacgg ccgcgacgcg agcagcatgc tcctcgtcga
     3241 ccgcgaccgg agggcgctcg tgcagttcgg gcgtcgggga aagtggtggt ccccgggcga
     3301 tccggtctcc gtgatccctc tcgacgcgcc cggactcatt ccgctcgcgg cccggaccgc
     3361 acgcgtcgtg aacgttcccg acgtctcgcg cgccccggac tacgtggtcg ggtggcccga
     3421 ggcgcgctcg gagctcgtcg tcccgctcgt cctcgacgag aaggtgatcg gcgtcttcga
     3481 tctccagtcg ggggaaccgg cggcgttctc ggacgccgac gcgcggaaca tcgcggcctt
     3541 cgccgagcgt gcggccctcg cgttgcgcct cgcggagctc gtcggggcct tcgagcagcg
     3601 gacccgcgtg ctcgaggccg tggcgcgcgc gacgcagctc ctgaacttcc gcctccatgc
     3661 tccggacgtc ctcacgtccg tcgtggagga gacgacgcgg gcatttcccg ccgcggacgg
     3721 ggccgtggcg tgggtcgcgg atccgccggg ccggaacctc tccgtcgccg cggtgttcgg
     3781 cgtcggcgtc atgacgctcc acggcggcgg gtcggggccg cgtcccgtcg ggcgcctgcg
     3841 gagcgccggg cgcgcgttcc gcgagaaccg gcccgtgttc acggcggtga acggcctcga
     3901 cgagctggcg gaaggcgatc cggtcgagag ccgcgcgaag gcgcgcgccg cggtcgcgga
     3961 cgccgacgtg cgcgcgctca tggccgtgcc gatccgcgtc ggcgaccaga ggctcggcgt
     4021 cctcgaggtc atgtcgtacc gcacgggagc cttcacgcgc gaggacggcg ccacgctcac
     4081 gctcctcgcc gagcagtgcg cgatcgcctt gcgcaacgcg cggctcatcg aggagctcca
     4141 gcggagtaac cgtctcaagg acgacttcct cgcgaacctc tcgcacgagg tccgcacgcc
     4201 gctcacgggg atcgtgggct gggccgaggt cctcctcgac tcgcacggcg gcgatccgcc
     4261 cgtgaagcgc gccctggagg cgattctcgg acagtccgac acgctcagcc gcatgctcgg
     4321 agacctgatc gacctctcgc ggatcgacaa cttcgggctc gagctccggc gcgagcgcgt
     4381 gcggctctcc gagacgattg cgggcgcact ggacgccgtc gctccgggag ccgccaaacg
     4441 gggcgtcgcg atccgctgcc acatcgagcc cgacctgccc gcgatggagg gcgatccggc
     4501 gcgcctcaag caggtcgtct ggaaccttct cgccaacggc gtgaaattct cgccgaccgg
     4561 gggcacggtc catgtgaccg tggcacgcac cggggacggc gggatcgagc tctccgtcga
     4621 ggacgagggc cacgggatcg acccggaatt cctcccgcac gtcttcgagc gattccggca
     4681 ggaagagacg tcctcgagcc ggcgcttcgg gggcctcggc gtcgggctct cgatcgcgcg
     4741 ggccatcgtg gaggcgcacg gcggaacgat cgccgccgag agcgaaggcc gcgagagggg
     4801 gagccggttc cgagtcgggt ttccgcccga gcgggtcgtc gggccggtcg tccgcgggac
     4861 ggttcacggg agcgggcggt gagtccggac ccgcgttcgc tcgaggagcg cctccggcag
     4921 gccgaggagc gcatcgcagc gctcaaccgg atcggaatcg cgctgtccgc cgaacgcgac
     4981 ctcgagaagc ttctcgagac gatcctcacg gagtcgcgcc gtttctccgg gtcggaagcg
     5041 ggctccctct atctcgccga ggacgcagcc ggcggcggac ggcggctccg cttcaagctc
     5101 gcccagaacg acgccgtctc gttcgccttc aaggagcgca cggtgccggt cgacgagggc
     5161 agcctcgcgg gctgggtcgc cgtccacgga gtcccgctcg tcctcgagga cgcctacgcc
     5221 atcccgaagg acgccccgta ccggcacaac gacgccttcg acgtcgcgac cggcttccgg
     5281 acgcgcgcga tgctcgtcgt ccccatgaag gaccatctcg gggacctcgt gggtgtcctc
     5341 cagctcatga accggctcgg gcccgccggc ccggagccgt atccggagga cctcgttccg
     5401 ctgctccttt cgctcgcgac gcaggcggcg gtctgcctga aggcgaacca gctcacggcg
     5461 tccatccgca ggctcttcga ggacttcgcg caggcggcca tcgtggccgt cgagcagcgc
     5521 gacccgacga ccgccggcca cagcaaccgt gtcgcggctc tgacggtcga gctcgccggc
     5581 ctcgtggacc gggcgccgga cgggccgtac gccgccgtgt cgttctcgcg cgaggagatg
     5641 cgcgaaatct tcacggccgc gctcctccac gacttcggca agatctcgat ccccgagcgc
     5701 gtcctcgtca aggcgaagaa gatcgaggag gacgggttgc gccggatccg cgaccgcttc
     5761 gactacgtcc tcgccgcatc ggagtccgag gacgcgcgcg cgctgctcga gcgcctcgcg
     5821 aaggacggcc ggcccgtgac ggaccgcgac ctggccgcgc tcgcggaggc ggccgatgcg
     5881 cgaagccggg aattcgaggc ggcgttcgag gaggtcgcgg ccgcgaacga gccgacgatc
     5941 cttccgaagg acgcacgcgg cgcgctcgag cggctcgtcg cgcgcacgtg gcgcgaccgg
     6001 cgcggccgga cggcgacgct tctcctgccc gaggagttcc gtttcctctc gatcgcgcag
     6061 ggcagcctct cgcaggagga gcggctcgac gtcgagagcc acgtgacgca cacgtggcgt
     6121 ttcctctcga cgattccgtg gacgccggac ctcgcgaggg tgccccagat cgcgtacgcg
     6181 caccacgaga agctcgacgg ctcgggttac ccgaggcgcg tcgcctcccc ggagattccg
     6241 ccggcggcgc gcgcgctcac ggtctgcgac atctacgacg cgctcacggc ctcggaccga
     6301 ccgtacaaga aggcgatgcc gcacgaccgc gcgctcgagg tcctcgcggc cgaggccgaa
     6361 aaggacaagc tcgacacgtg gctcgtgaag gccttcatcc aggggcgggt ctggctcgcg
     6421 ctcgggcgtt gagggcggcg ctcgccgccg cgctccttct cggagcgccg cttgcggcag
     6481 ccgctcccgc gaggtggcat ttcacgttcg ggaagacgtg ggagatttcg attctcttcg
     6541 aaagggagga gaagggagac tcgacccgtc tcctcgtctc agcgccggca ggacgcttcg
     6601 tgttcctttc ctctcaggat ccctccggtc gcgactccac ggagagcatt cgccgcctcc
     6661 cggacggcga gacgttcacc cgccgcctcg tgctttcctc attcaaagaa atccccgcgt
     6721 gctcttcggt ccgcgccccg gacgcgtgcg tcgtcttcgc cggcgcccgc ggcacatttg
     6781 ccgcgggatt ctccgagttc gcgggagacc gcgctcccgc ggcgcgcgag aaagcgtccg
     6841 cgctcgtgtc gcccggcatg cgcgacgcgc tcttcgctct cgccccgatc ctccccgctt
     6901 ttctcgagtt cgggtcctat tcgaaggact tcctcgcgct cgtctggccc gagcgcttct
     6961 ccgcgccgca gaagctcgtc cgcggcgagc gaaagcccgg ctgcgacttc gacgcgacgt
     7021 tcggcttccc gtgctcggag gaggagaagg cccgcgaggc caggcgcttc gcggacgccg
     7081 gcgccgcaac ccggccctga ccgcgccccg aacgccgccc gccggcaatg cgagaatccg
     7141 ggaagggcgc attcgatgac cgggccgaac cccacaccgc cgggcgacgt gaccgggctc
     7201 ctcctcgcct ggaacggggg agaccgcgag gcgctcgaga ggctcgtccc cctcgtctac
     7261 gaggagcttc ggaagcgcgc gcagcgcgcc ctcgcgaagg agcggcgcgg ccacacgctc
     7321 cagcccacgg ccctcgtcca cgaggcctac atgcgcctcg tcgaccagaa ccgcgtgccc
     7381 tggcagaacc ggttccactt ctacgccgtc tcggcgcggg cgatgcggca ggtcctcgtc
     7441 gaccacgccc gccgccgcct cgccgacaag cgggacggcg gtaagacgtt cgttcccctc
     7501 tcgcgggcgg gcgccgaggc cgcgacgccg ccgcccagcc tcgacgtcct cgcgctcgac
     7561 cgcgcgctcg agaggctcgc gaccctcgac ccgcggcagg cgaagctcgt ggagctgcgc
     7621 gtgtacggcg gcctcacgat cgacgaggcc gccgaggcgc tcggcgtctc ggcggcgacc
     7681 gtgagccggg agtggcggca cgcggaggcc tggcttcacc gcgagatggc gggctccggg
     7741 gcgccgtgac tcccgagctc tacgcgcgcg ccaaggagat cttcctctcc gcgcaggagg
     7801 ccgcggagga ggagcgcggg acgctcgtcg cggaacggtg cggcggcgac gagccgctcc
     7861 ggcgcgaggt cgaggccctg ctcgcctcgg accgggcggg cgcgggcttc ctcgaccggg
     7921 ccgcgttgcc ggtcccgccg tgggacgaag tggagcccgc gccgcccacg cgcgtcgggc
     7981 cgtatcgcgt cgtcggcgag atcgggcgcg gcggcatggg ctcggtctac ctcgccgagc
     8041 gggcggacga cgcctacgcc gggcgcgtcg cgttgaagct cgtccggcgc gggatggaca
     8101 ccgaggacat cctccgccgc ttccggacgg agcggcagat cctcgcgtcg ctccagcacc
     8161 cgaacatcgc gcgcctcctc gacgggggca cgaccgagga cggccgcccg tacttcgtga
     8221 tggagtacat cgaggggcgg acgctgacgg aggacgcgcg ggcccgcgcg cttccgctga
     8281 aggatcgcct cacgctcttc cgcgtcgtct gtgccgccgt ccagcacgcg caccgcaacc
     8341 tcgtcgtgca ccgggacctc aagccgtcca acgtcatggt cacggcggac ggcgccgtga
     8401 agctcctcga tttcgggctt gcgaaggtcc tcgggaccga ggctccggcg ccggagcgga
     8461 cgacgggggc caccgccatt ttcacgcccg agtacgcgtc gcccgagcag ctcgcgggcc
     8521 gcgccatcac gacgtcgacg gacgtctact ccctcggcgt cgtcctctac gaactcctcg
     8581 ccgacgccca cccgtaccgc gaggacgcgg cctcgccggc gcgcctctgg caggccgtct
     8641 gcgagacgga cccaccgccg ccctccgccg tcgccgcgcg cacgggagga ccggccgcgc
     8701 gccgggcgcc cgcgctcgcg ggcgatctcg acgcgatcgt cctgaaggcg aggcgcaagg
     8761 atccctcgcg gcggtacgcg accgtggac
//
LOCUS       DAOMFY010000152         8775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2449_length_8775_cov_7.962385, whole genome shotgun sequence.
ACCESSION   DAOMFY010000152 DAOMFY010000000
VERSION     DAOMFY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2449_length_8775_cov_7.962385"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLB01_12350"
     CDS             <1..445
                     /locus_tag="PLB01_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78136.1"
                     /translation="PDERANRTVALGAGSPTPAPLPVPAAAPSPTRVPAPPIVEEPGR
                     SEPDTGEAPAAAGSVHRKPGVAPRPVSVAKAPSYTVVALDGKSLTLRRAEGTTVTFPL
                     AKKADVPKGLAPGQSVTIKTRTENGKKVVSRVREASDAPVLTNVN"
     gene            complement(453..4028)
                     /locus_tag="PLB01_12355"
     CDS             complement(453..4028)
                     /locus_tag="PLB01_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78137.1"
                     /translation="MPTPLRIGLLVFVLLSLALLPGCGIPEDLNGDGIADGIQDPNAV
                     VLRAPSRPLDQPPGGVVAPFVDASVASLDPSGDGSLPFPFIVPIVLTSPSATDEVFTT
                     DGVDGGGAGEVSRANFSFAFGLDFFPSAFTYLRVEGAAPSARSALAQAHRGSGVVAVG
                     NRILFASQEDLSLQSFTCPLLDGGNTATCATQLDDDAFFVGGVNATGDGRAGGYVCSR
                     ASGACTPSAGFDPSYSFGDGVTASAPASVAAGNLRTSFVSNSVILAGTAAKGVYRSTD
                     SGRTFVRVTSGLPSFLAVTRFAAAGSAVVAATGSGLYRSTDQGASFTKITAGLPAGAV
                     ADVRAAGAALYAAVNTATPGVFKSTDSGQTWQATGSGLAATRINSLASNANGIFAATN
                     GGLFKSGDGGASWRAYGKGLTVSNHLAMLVSENTVFTGTFANASGVLRSADNGLTWGA
                     PGGPLGFKIIRALWASGSVVLASGENGLYKSTDGGLSFAAIAPASVGLPTSGAYLNSF
                     VTAGNAVLGLLNTGGVFRSTNLGDTWTASSLGLPAGVAGASLAANGNTVVMGSSSALY
                     RSLDGGATWSAATPPTSATPYSVYALLFSGGTLYAGTYGFGAPDTHGLFRSNDAGTTW
                     TRLSNGIPSNTPVYALAAADGRIFAGLRRGLFASSDGGDSFQVFERRLATKSIFALAA
                     GPAGPRAPAGATRLYVGTGTGGLYIPLVPPLAQRLVPIVLDVDNGSGAHYTTELALTN
                     RGSRPAAVTYRYTASIGSGPGIATASLAAGQQLIVPDAIAALRAKGVPIPPGNAGGTL
                     LVTFDGLAEAEAVSVTARTTTATGAPQPAGAAGLAYAALDHSRSPTGKQTLYGLRSSA
                     SDRSNAAVFNMGSAPVTLRVTAWSGDGSGASAVIETVTLPSFGWKQYTRILEGPGYAT
                     GWVTIERVGTTGAFGSYAVINDNGTSDGSFVLPADDLPRPQFLNVPVLVESSSFVSEL
                     VLTNASAKNATFVLTYAGGAGGTANVAVPARTQLIFPGAIAELRSRGIAVGPAGAATY
                     AGALHVFVQGAPVNETFAGARTASPSPAAGQFGLFTPAFAAGAEANEAAWIYGLRADA
                     NNRSNVAFVNTGATPGAGSVTLQVQAYDGDAVGAAAGAPDTVTLGPGQWSQLSGFLAS
                     KGVRNGWVRITRTAGSAPWIAYGVVNDGGGPGQRTGDGAYVPMSR"
     gene            4165..4902
                     /locus_tag="PLB01_12360"
     CDS             4165..4902
                     /locus_tag="PLB01_12360"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQQ78138.1"
                     /translation="MFEPAPAPRRRPMFAVSTAAHAALAAVLVVPPLFATPEPPEPDG
                     FVHITQLPPLATDAPIRETVVFLRKGNWGGPAAPSARATSAPRNAGPRLTQPIAVPEV
                     LPPATGDAPQLPFEEWGERSEPTPRGGGREDGTEEGGDGGCSGCNAISATARGVTPPV
                     AIETIAPPYPELARRAHVEGVVVLEAVIGGDGSVRDVRVLRGASPLLDPAALEAVRRW
                     RYRAATIGVRPVAVYLQVVITFSLRNL"
     gene            4915..5604
                     /locus_tag="PLB01_12365"
     CDS             4915..5604
                     /locus_tag="PLB01_12365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQQ78139.1"
                     /translation="MILFENADVLALDKPAGVSMATSSRPGSAEEAVRRLLAAGGLAA
                     EEPMPLLVHRLDVGTSGVVLLAKNDTAHRALSRALQQKTAQKTYRALVWGRPVPARGT
                     VDLPLARDPKDGRKMRAGKDGKPSVTRYETLKRYAGLADLRVFPETGRTHQIRVHLSA
                     KGHPIAGDDFYGGATRWRGVKDPALRGALRRLSRPLLHAERIAVPEMGIDVASPLPKD
                     YEEILRLCGGC"
     gene            complement(5724..8624)
                     /locus_tag="PLB01_12370"
     CDS             complement(5724..8624)
                     /locus_tag="PLB01_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ78140.1"
                     /translation="MTAGPALAQSATGSISGTVLDTAGAPLPGAAVAAKNAKTGAVRN
                     ATSNASGAFVFPLLPVGVYDVTATLQGFTASRTGRAEVSVGADTSLKLVMAIAGVSST
                     IAVTAETPVVETTKSEQASAVNEAYIQNLPTNGRNFIDFVLTTPGVSKDPRLGDISFA
                     GQRGTLNSLVIDGANNDNTFFGQALGRTGSGRAPYQFSQDAVKEFQVNRNAYTAEYGR
                     AGGAVINVVTKSGTNEFHGTAFEFYRDKGMNANDYINVINGRPISNYHYNQFGASLGG
                     PILKDQLFFFANYDGQRNTNGQPVILNTSSLNLSDPNTAAGLAKLTPKATPYDRGQNQ
                     DVFLLKADWNVAPTAQVSMRYNRQRFTGVNNESGGSTIAEEHSGDSLVNTDTVTASVT
                     NTFGSSVFNELRGQYAKDSEPGLANSADPEALISDGSFSFTIGRNFFSPRETTITRYQ
                     VADAATFIFGNHSFKAGFDWSRDLILNWFPGNFSGSYSFNSVANYNRGISSSYTQAFP
                     GAGTTGPYTNPDLSEIAFFAQDEWRAMPNLTLNLGVRYDKQGIAQPIVRNPDAQLLAA
                     GIDTSVVPEDTNNVALRAGFAWTPKGQDKTVVRGGYGMFYGRTPSIMIGTAHSNNGIN
                     VQTLTFQGASQPTYPNIFPSIPSGVVLPKPTIFVFDPNFENPLVHQASLGIEHAVTPD
                     VAVALSYLYAKGTHLQRSTDINVGSAGTTTYTDASGNTYTILKYNAADRPFSNFGRVI
                     QFQSTADSEYNGATLEINKRFSKNWQARIAYTFSKVIDTRPDATAVVPASGDDAKFAS
                     DPRNFAVDRTVGDTDVPHRLVISAYWSLPYFRGAGGWKEAVLGGWSLSGIFTVASGQP
                     YSRTVTPNTDLNGDGNTRNDFAPGYSRNTERYPMFMSWDPRVTKDIPIGPVNLQLIAE
                     AFNVLNRSNVVAVTQTYWRVNAGQTLAPVTAYGTPTSSAGPRIIQLAAKLIF"
BASE COUNT         1290 a         2914 c         3251 g         1320 t
ORIGIN      
        1 ccccgacgag cgcgcgaacc gcaccgtggc gctgggggcc ggctcgccga ctccggcgcc
       61 gctgcccgtc ccggcagccg cgccttcgcc gacgagggtc ccggctcctc cgatcgtcga
      121 ggagcccggc cggtcagaac cggacacggg cgaggcgccg gcggcggccg gcagcgtgca
      181 ccggaagccc ggcgtcgcgc cgcggcccgt gagcgtcgcg aaggcgccct cgtacaccgt
      241 cgtcgccctg gacgggaaga gcctcacgct ccggcgggcg gaagggacga ccgtgacgtt
      301 cccgctcgcg aagaaggcgg acgtcccgaa gggcctcgcg cccgggcagt ccgtgacgat
      361 caagacccgg accgagaacg ggaagaaggt cgtgtcgcgc gtgcgcgaag cgagcgacgc
      421 gccggtcctc acgaacgtga actgagccgt tctcaccgcg acatcggcac gtacgctccg
      481 tctcccgtgc gctggcccgg gccgcctccg tcgttcacga cgccgtaggc gatccacgga
      541 gccgaccccg ccgttcgggt gatccggacc cacccgttcc gcactccctt cgaggcgagg
      601 aagcccgaga gctggctcca ttggccgggc ccgagcgtca cggtgtccgg agcgcccgcg
      661 gccgcgccga ccgcgtctcc gtcgtaagcc tgcacctgga gagtgacgga gcccgcgccc
      721 ggcgtggcgc ccgtgttcac gaacgcgacg ttggagcggt tgttcgcgtc cgcgcgcagg
      781 ccgtagatcc aggcggcctc gttcgcctct gcgcccgcgg cgaacgccgg cgtgaagagg
      841 ccgaactgtc ccgccgccgg agagggcgaa gccgtccgcg cgccggcgaa ggtctcgttc
      901 acgggagcgc cctgaacgaa cacgtggagg gcgcccgcat aggtcgcggc gccggcgggg
      961 ccgaccgcta tcccgcggct ccggagctcg gcgatggcgc cggggaagat gagctgcgtc
     1021 cgcgccggaa cggcgacgtt cgccgtaccg ccggcgccgc ccgcgtaggt caggacgaag
     1081 gttgcgttct tcgcgctcgc gttcgtgagc acgagctccg agacgaacga gctcgactcg
     1141 acgaggacgg gaacgttcag gaactggggc cgcgggagat cgtcggccgg gagcacgaac
     1201 gagccgtcgc tcgtgccgtt gtcgttgatg accgcgtaac tgccgaaggc gccggtcgtc
     1261 ccgacgcgct cgatcgtcac ccagcccgtg gcatagccgg ggccttcgag gatccgcgtg
     1321 tactgcttcc agccgaagga gggcagcgtg acggtctcga tcacggcgct ggcgccgctg
     1381 ccgtcgccgc tccaggccgt tacgcgcagc gtgacgggcg cggagcccat gttgaagact
     1441 gccgcgttac tgcggtcgga cgccgaagag cgcagcccgt agagcgtctg cttcccggtc
     1501 ggggaccggg agtggtcgag cgccgcgtag gcgaggcccg cggcgccggc gggctgcggc
     1561 gctccggtcg ccgtcgtcgt gcgcgcggtg acggacaccg cttcggcctc ggcgaggccg
     1621 tcgaacgtca cgaggagagt gccgccggcg ttgccgggcg ggatcgggac gcccttggcg
     1681 cgcagggcgg cgatcgcgtc gggcacgatc agctgctgcc cggccgcgag cgaggcggtc
     1741 gcgatgccgg ggccgcttcc gatcgaggcg gtgtaccggt acgtgacggc ggccggccgc
     1801 gagccgcggt tcgtgagcgc gagctcggtc gtgtagtgcg caccgctgcc gttgtcgacg
     1861 tcgaggacga tcggcacgag acgctgcgcg aggggcggca cgagagggat gtagaggccg
     1921 cccgtgccgg tcccgacgta cagcctcgtg gccccggccg gcgcgcgcgg ccccgcgggc
     1981 cccgccgcga gtgcgaagat cgacttcgtc gcgagcctgc gttcgaagac ctggaaggaa
     2041 tcgccgccgt cggacgaggc gaagaggccg cgccgcaggc cggcgaagat gcggccgtcc
     2101 gcggccgcga gcgcgtagac cggcgtgttg gaggggatgc cgttcgagag gcgcgtccac
     2161 gtcgtgccgg cgtcgttgct ccggaagagg ccgtgcgtgt ccggggctcc gaagccatac
     2221 gtcccggcgt acagcgtgcc gccggagaag aggagcgcat agaccgagta aggcgtcgcg
     2281 gatgtcgggg gcgtcgcggc gctccacgtc gccccgccgt ccaggctgcg gtagagcgcg
     2341 ctcgaagagc ccatgaccac ggtgttgccg ttcgcggcaa gcgacgcgcc cgcgacgcct
     2401 gcggggaggc cgaggctcga ggctgtccag gtgtcgccga gattcgtgct ccggaagact
     2461 ccgcccgtat tgaggaggcc gagcacggcg tttcctgcgg tcacgaacga gttgaggtac
     2521 gcgccgctgg tcgggaggcc gaccgaggcc ggcgcgatcg cggcgaagct caggccgccg
     2581 tccgtgctct tgtagagacc gttctcgccc gacgcgagga cgacgctgcc cgaggcccag
     2641 agagcccgga tgatcttgaa gccgagcggg ccgccgggcg cgccccaggt gaggccgttg
     2701 tcggccgaac ggaggacgcc actcgcgtta gcgaaggttc cggtgaagac ggtgttttcg
     2761 gacacgagca tcgcgaggtg gttcgagacc gtgaggccct tgccgtacgc cctccaggaa
     2821 gccccgccgt cgccggactt gaagaggccc ccgttcgtcg cggcgaagat gccgttcgcg
     2881 ttcgaggcga gggagttgat ccgcgtcgcc gcgagaccgg atcccgtcgc ctgccacgtc
     2941 tgcccggagt ccgtgctctt gaagacgccc ggcgtggccg tgttcacggc ggcatacaac
     3001 gcggcgcctg ccgcgcgcac gtcggcgacg gcgccggccg ggagacccgc cgtgattttc
     3061 gtgaaggacg cgccctgatc cgtgctgcgg tagaggcccg agcccgtcgc cgcgacgacc
     3121 gcgctccccg cggccgcgaa ccgcgtcacg gcgaggaagg acgggaggcc cgaggtgact
     3181 cggacgaacg tccggcccga gtcggtgctc cgatagacgc ccttggccgc ggtgccggcg
     3241 aggatgacgg aattcgagac gaacgacgtt cggaggttcc ccgccgcgac gctggccggc
     3301 gcgctggcgg tgacgccgtc gccgaaagag taggacgggt cgaagcctgc ggatggggtg
     3361 caggcgccgc tcgcgcggct gcacacgtag ccgccggccc ggccgtcccc ggtcgcgttg
     3421 acgccgccca cgaagaacgc gtcgtcgtcg agctgggtcg cgcacgtcgc cgtgttgccg
     3481 ccgtcgagga gcgggcacgt gaagctctgg agcgagaggt cttcctggct cgcgaagagg
     3541 atcctgttcc cgaccgccac gacgccgctc ccgcgatgcg cctgcgcgag cgcgctccgc
     3601 gcggacgggg cggccccctc gacccgcagg tacgtgaacg cggacgggaa gaagtccagg
     3661 ccgaaggcga acgagaagtt cgcccgcgac acctcgcccg cgccgcctcc gtccacgccg
     3721 tccgtcgtga agacttcgtc ggtcgcgctc gggctcgtga ggacgatcgg gacgatgaac
     3781 gggaagggaa gagagccgtc gccggacggg tcgagggacg cgacggacgc gtcgacgaac
     3841 ggagctacga cgcctcccgg cggctgatcg agaggccggc tgggagccct gaggaccacg
     3901 gcgttcgggt cctggatgcc gtcggcgatg ccgtcgccgt tcaagtcctc gggaattccg
     3961 cagcccggga ggagggccag cgaaagcaga acgaaaacca gcagcccgat tcgaagcgga
     4021 gtcggcatga gagacctccg gtcgattcct gtcttggatt tccggagact tcataacccg
     4081 atccggtccc ggtgtcgaga acgaatctcg tttgcgggaa cttccacgcc tccgcccggg
     4141 tcggatcggg gcgaggaggc cgccatgttc gagcccgctc ctgcgccccg ccggaggccc
     4201 atgttcgccg tctcgaccgc ggcgcacgcg gcgcttgcgg cagtcctcgt cgtgccgccg
     4261 ctgttcgcga cgccggagcc gccggagccg gatgggttcg tacacatcac ccagctgccg
     4321 ccgctcgcga ctgacgcccc gattcgggag acggtggttt ttcttagaaa gggtaattgg
     4381 ggcggaccgg cggctccgtc ggcgcgcgcg acgagtgccc cgcggaacgc cggaccgcgg
     4441 ctcacccagc cgatagccgt tccggaggtg ctgccgcccg ccacgggcga cgccccccaa
     4501 ttacctttcg aagaatgggg cgagcgcagc gagccaactc ctcgcggtgg cggtcgagag
     4561 gacggcaccg aagaaggcgg ggacggcggc tgctcgggat gcaacgcgat ctccgcaacg
     4621 gcccgaggcg tgacgccgcc ggtggcgatc gagacgatcg cgcccccgta ccccgaactc
     4681 gcgcggcgcg cgcacgtgga gggcgtcgtc gtcctcgagg cggtgatcgg tggcgacgga
     4741 tcggtgcgcg acgtgcgcgt cctgcggggc gcgagcccgc tcctcgaccc cgccgcgctc
     4801 gaggccgtga ggcgctggcg ctaccgcgcg gccacgatcg gagtgcggcc cgtcgccgtg
     4861 tacctgcagg tcgtgatcac gttctcgctc cggaacctgt gacataacgc cggcgtgatc
     4921 ctgttcgaga acgccgacgt cctcgcgctc gacaagcccg cgggcgtctc gatggcgacg
     4981 tcgtcgcgcc ccggctcggc ggaggaggcc gtgcggcggc tcctcgcggc gggcggcctc
     5041 gcggcggagg agccgatgcc gctcctcgtc caccgcctcg acgtcgggac gagcggcgtc
     5101 gtcctcctcg cgaagaacga cacggcgcac cgggcgctct cccgcgctct gcagcagaag
     5161 acggcgcaga agacgtaccg cgcgctcgtg tggggccgcc cggtgccggc gcgcgggacc
     5221 gtcgaccttc cgctcgcgcg ggacccgaag gacgggcgga agatgcgcgc cgggaaggac
     5281 ggcaagccgt ccgtgacgcg ctacgagacg ctcaagcggt acgcgggcct cgcggacctg
     5341 cgcgtcttcc ccgagacggg gcgcacgcac cagatccgcg tccacctctc cgcgaagggc
     5401 cacccgatcg cgggcgacga cttctacggc ggcgcgacgc gctggcgggg cgtcaaggac
     5461 ccggcgctgc gcggggcgct tcggcgcctg tcgcgtccgc tgctgcacgc cgaacggatc
     5521 gccgtgccgg aaatgggcat cgacgttgcc tcgccgcttc cgaaggatta cgaggagatc
     5581 cttcggctat gcggcggctg ctgatgcagc cgccgataga tgagaaatct gcggccgttc
     5641 cgccggtgtc aagggtctgc gaccgctacg cggcgcgcgc agcgcgccct tgactccggc
     5701 tgaacggcgc atatctcctc ttcctagaaa atcaactttg ccgcgagctg gatgatccgc
     5761 gggcccgccg agctcgtcgg cgtgccgtag gccgtgacgg gcgcgagcgt ctgtccggcg
     5821 ttcacgcgcc agtacgtctg cgtgacggcg acgacgttcg agcggttcag gacgttgaag
     5881 gcctccgcga tgagctggag gttgaccggc ccgatcggga tgtccttcgt gacgcgcggg
     5941 tcccacgaca tgaacatcgg ataccgctcg gtgttgcggg agtagcccgg cgcgaaatcg
     6001 ttccgggtgt tgccgtctcc gttgaggtcg gtgttgggtg tgaccgtgcg cgagtacggc
     6061 tggccggacg cgaccgtgaa gatgcccgag agcgaccagc cgccgaggac ggcctccttc
     6121 cagccgcccg cgccgcggaa gtacggcagg ctccagtacg ccgagatcac gagccggtgc
     6181 ggcacgtcgg tgtcgcccac ggtgcggtcc acggcgaaat tgcgcgggtc ggaggcgaac
     6241 ttcgcgtcgt cgccgctcgc cgggacgacg gccgtcgcgt ccggccgcgt gtcgatgacc
     6301 ttgctgaacg tgtacgcgat gcgggcctgc cagttcttcg agaagcgctt gttgatctcg
     6361 agagtggcgc cgttgtactc ggagtccgcc gtgctctgga actggatgac gcgcccgaag
     6421 ttcgagaacg gccggtccgc cgcgttgtac ttcaagatcg tgtacgtgtt tccgctcgcg
     6481 tccgtgtacg tcgtcgtgcc ggccgagccg acgttgatgt ccgtcgagcg ctggagatgc
     6541 gtgcccttcg cgtagaggta cgacagggcg acggcgacgt cgggcgtgac ggcgtgctcg
     6601 atcccgagcg aggcctggtg gacgaggggg ttctcgaagt tcgggtcgaa gacgaagatc
     6661 gtcggtttcg ggaggacgac gcccgagggg atcgacggga agatgttcgg gtacgtcggc
     6721 tgcgaggcgc cctggaacgt gagcgtctgg acgttgatgc cgttgttcga gtgggccgtg
     6781 ccgatcatga tcgacggcgt gcggccgtag aacatcccgt agccgccgcg cacgacggtc
     6841 ttgtcctggc ccttcggcgt ccacgcgaag cccgcgcgga gggcgacgtt gttcgtgtcc
     6901 tcggggacga cgctcgtgtc gatgccggcc gcgaggagct gcgcgtcggg attccggacg
     6961 atcggctggg cgatgccctg cttgtcgtaa cgcacgccga ggttcagcgt caggttgggc
     7021 atcgcgcgcc actcgtcctg ggcgaaaaag gcgatctccg acaggtcggg gttcgtgtag
     7081 gggcccgtcg ttcctgcacc cgggaaggcc tgggtatagg agctcgagat cccccggttg
     7141 tagttcgcga cggagttgaa cgagtagctg cccgagaagt tacccgggaa ccagttcagg
     7201 atgaggtcgc ggctccagtc gaagccggcc ttgaacgagt ggttcccgaa gatgaacgtc
     7261 gcggcgtcgg cgacctggta gcgcgtgatc gtcgtctcgc gcgggctgaa gaaattccgg
     7321 ccgatcgtga acgagaacga accgtccgag atcagggcct ccgggtcggc gctgttcgcg
     7381 aggccaggct cggaatcctt cgcgtactgg ccgcgcagct cgttgaagac ggacgagcca
     7441 aacgtgttcg tgacggaggc cgtgaccgtg tccgtgttca cgagcgagtc accggagtgt
     7501 tcctcggcga tggtcgagcc gccgctctcg ttgttgacgc ccgtgaaacg ctggcggttg
     7561 tagcgcatcg agacctgggc ggtcggcgcc acgttccagt ccgccttgag gaggaagacg
     7621 tcctggttct gaccgcggtc gtagggggtc gccttcggcg tgagcttcgc gaggcccgcc
     7681 gcggtattcg ggtcggagag attcaggctg gacgtgttca ggatgaccgg ctggccgttc
     7741 gtgttgcgct ggccgtcgta gttcgcgaag aagaagagct ggtccttcag gatcgggccg
     7801 ccgaggctcg cgccgaactg gttgtagtgg tagttgctga tcggccggcc gttgatcacg
     7861 ttgatgtagt cgttcgcgtt catccccttg tcgcggtaga actcgaacgc ggtgccgtgg
     7921 aactcgttcg tgccggactt cgtgacgacg ttgatcacgg cgccgcccgc gcggccgtac
     7981 tcggccgtgt aggcgttgcg gttcacctgg aactccttca cggcgtcctg gctgaactgg
     8041 tacggcgcac ggcccgagcc cgtgcggccg agcgcctggc cgaagaacgt gttgtcgttg
     8101 ttcgcgccgt cgatcacgag gctgttcagc gtgccgcgct ggcccgcgaa gctgatgtcg
     8161 ccgagccgcg ggtccttgga gacgcccggc gtcgtcagaa cgaagtcgat gaagttgcgg
     8221 ccgttcgtcg ggaggttctg gatgtacgcc tcgttgacgg ccgatgcctg ctcgctcttc
     8281 gtcgtctcga cgaccggagt ctcggcggtg accgcgatcg tggaactcac gccggcgatc
     8341 gccatgacga gcttcaggct cgtgtccgcg ccgacgctga cctcggcccg gccggtcctg
     8401 gacgccgtga acccctggag cgtcgccgtc acgtcgtaga cgcccacggg aagaagcgga
     8461 aagacgaatg cgccgctcgc gttcgacgtc gcgttgcgca ccgcgcccgt cttcgcgttc
     8521 ttcgcggcga cggcggcgcc cgggagcggc gccccggccg tgtcgagcac ggtgccggag
     8581 atactgccgg tggcgctctg cgcgagggcg gggccggcgg tcacgcacaa ggcgatcgcg
     8641 acaaccaggg cgagtgagac tcgatacgca cgaaccatct gaaactcctc catcaagaac
     8701 gaattcaagg cgaagccgtc ggcgcgaacg ggtggcgatc cgggaacgcc gggaagacta
     8761 ttacaaaacc cgggg
//
LOCUS       DAOMFY010000153         8728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2465_length_8728_cov_9.415888, whole genome shotgun sequence.
ACCESSION   DAOMFY010000153 DAOMFY010000000
VERSION     DAOMFY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2465_length_8728_cov_9.415888"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="atpD"
                     /locus_tag="PLB01_12375"
     CDS             complement(<1..1196)
                     /gene="atpD"
                     /locus_tag="PLB01_12375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQQ78141.1"
                     /translation="MATEGRVVQVIGPVVDVEFPAGKLPTILNAVRIVDDEKLSTVPI
                     DIITEVAQHLGENRVRCISMKPADGLVRGMKAIDTGAPITIPVGPETLGRVLNVIGEP
                     VDEMGPVTTTEHWPIHREAPAYEDQSTSVEMFETGIKVIDLLEPYTRGGKTGLFGGAG
                     VGKTVLIMELINNVAKAAGGYSVFAGVGERTREGNDLWLEFTESGIIDPKDWKKSKAA
                     LIYGQMTEPPGARLRVGLTGLTAAEYFRDKEGKDVLLFIDNIFRFTQAGSEVSALLGR
                     MPSAVGYQPNLATEMGELQERITSTKRGSITSVQAIYVPADDLTDPAPATAFAHLDAT
                     TVLSRQIAEIGIYPSVDPLASTSKILSPLTVGEEHYKVARAVQQVLQKYKDLQDIIAI
                     LGIDELS"
     gene            complement(1226..2110)
                     /gene="atpG"
                     /locus_tag="PLB01_12380"
     CDS             complement(1226..2110)
                     /gene="atpG"
                     /locus_tag="PLB01_12380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQQ78142.1"
                     /translation="MANLIDIRRRIRSVKNTQQITKAMKMVAASKLRRAQDRVLSARP
                     YATTLEATLASVAARVPANEDGTPAHPLLQGREEKNVVVVAVSGDKGLAGGFNTNVNR
                     AVGAFLREEKEKGVSVRLIALGKKSNDFWKRRPVEILDARPGLFSKLNMAVASEIARD
                     LGARFVAGEVDAVYVVYNEFRSVISQIVRTKKLLPVSLEAGAAAPGAGGTDYLYEPGP
                     DVILGTLVPRFLEFQMYRVLLESNAAFFAAQMTAMDSASKNAGEIIDGLTLTYNRARQ
                     ARITKELIEIVSGAAALD"
     gene            complement(2130..3665)
                     /gene="atpA"
                     /locus_tag="PLB01_12385"
     CDS             complement(2130..3665)
                     /gene="atpA"
                     /locus_tag="PLB01_12385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQQ78143.1"
                     /translation="MADITAQEITQLIKAQLAGVTQGVDVDEVGTVISVGDGIALVYG
                     LDKAMAGELIAFPNEVFGLALNLEESHVGCVLMGETSLVKEGDTAKRTKRIVSVPVGD
                     ALVGRVVNPLGIPLDGKGPIASKEFYPVERLAPGVVDRMPVKEPMQTGIKAIDAMIPI
                     GRGQRELIIGDRQTGKTAIALDAIINQKGKDVICVYVAIGQKNSTVAQVVQTLEKHGA
                     MDHTIIVAASASDPAPLQYIAAYAGCAMGEYFMYNGRHALVIYDDLSKQAASYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERASKLSKEKGGGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQIFLESDLFFSGQRPAVNVGISVSRVGGSAQIKVMRAISGTLRLDLAQFRE
                     LAAFAQFGSDLDKTTQAQLNRGRRLVEILKQNQYVPMDVGLQVSSVFAGTKGFLDDLL
                     VEAVLPFETSLHEYLKDQKKSLLDEIRVAGKLTKEIEEALSAAVAEAKKVFLAARPEA
                     KVA"
     gene            complement(3682..4233)
                     /gene="atpH"
                     /locus_tag="PLB01_12390"
     CDS             complement(3682..4233)
                     /gene="atpH"
                     /locus_tag="PLB01_12390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HQQ78144.1"
                     /translation="MAERFTRPYVDAFFAVAGSAEAVDAQLPALDAVAHAIESNTELS
                     KVLANPGVERTRRKALLDAVAAKVGVDALGGRLLDILLQNRRVHRLPAFLAAVRERLD
                     RERRTLEAHVTSARPLDDAVFEALRRMVEGRTGSTVRVVPSVDPSLLGGFVVSVGSAR
                     LDASLARRLEKARAALHALAPAS"
     gene            complement(4236..4781)
                     /locus_tag="PLB01_12395"
     CDS             complement(4236..4781)
                     /locus_tag="PLB01_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HQQ78145.1"
                     /translation="MISSILLGLAQEGHEAAKGFLGVPNLFWQVTNLAAFLGLLWYFL
                     RKPVAEFFGTRRTEVAKTLAKAEDDRKRAEALAAELASRLAQIETELANLRESARRDA
                     EAEHAALLKQTEEDAARFLARASTDVDNRVRAARAELTAYAGDLAVEVAREILAKNVT
                     PEDEKRLVAEGVAELSARTKG"
     gene            complement(4778..5206)
                     /locus_tag="PLB01_12400"
     CDS             complement(4778..5206)
                     /locus_tag="PLB01_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78146.1"
                     /translation="MQLPDLSLLIIMVLFWATYAVLRAWVFKPLGSILEEREKKAATA
                     TDALAKALENEKETLASIDRRLTETRREAVAARQASRNAANAKRQALLDEAREKARLA
                     AAAAQAKLDAEIAAARAELAKNVRTTAGEIASLALGRRLA"
     gene            5405..6049
                     /locus_tag="PLB01_12405"
     CDS             5405..6049
                     /locus_tag="PLB01_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78147.1"
                     /translation="MRAKQELIDRFVTTLEGADRLWDEKIAVLRPGEIACRPGCFGCC
                     VGLFAIGLPEALALRHAVSGLPAAARAAVLARATRAVEQSAATFPGDAAAGLLDPERS
                     EAAEEAWFLDARGTPCPALELPSGRCLAYAARPTTCRTYGLALRAGGETLVSACELNF
                     PAAPGGRVHSTAIAAEDLTAVDQALVEAAAEAGLPAGLETTIAHALTGAAFDIL"
     gene            complement(6124..6492)
                     /locus_tag="PLB01_12410"
     CDS             complement(6124..6492)
                     /locus_tag="PLB01_12410"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQQ78148.1"
                     /translation="MSTKKSGGTLNGFLDRGARLEGTLTFDDVFRIDGAFKGTIVSDA
                     ELVVGDGGLVEGEIRVGRLAVSGTVRGVIHARERIEVHAGGRLFAEVHAPALVVEEGA
                     VIQGAVEAGTARPAPEGAGG"
     gene            complement(6489..7352)
                     /locus_tag="PLB01_12415"
     CDS             complement(6489..7352)
                     /locus_tag="PLB01_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQQ78149.1"
                     /translation="MSRKTALGRGLSALIPTAKPGREAGGLRTVPLALLDANRRQPRR
                     RFDDDGIAELSRSIAKTGILQPVLVTREGERYRILAGERRVRAARLAGLSEVPVVVRE
                     GVADRDHLLIALVENVQRRDLTALEEAEAYRHLREDFGLTQEDVAERVGKDRATVANA
                     LRLLKLPAAVREALESGALTAGHARALLALSSAADQEALAKEVARRGLSVRAVEARVT
                     ALAKGGGAKKRKHRAVDADTRDAELRLARALGTKVEIRRKKRGGEVRIAFYSEEELIG
                     LFEKLSGGGEA"
     gene            complement(7400..8170)
                     /locus_tag="PLB01_12420"
     CDS             complement(7400..8170)
                     /locus_tag="PLB01_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019543431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQQ78150.1"
                     /translation="MKHRVLAVANQKGGVGKTTTAVSLAAALAALERPVLLVDLDPQG
                     NATGGLGVDKAELARTTYDWLLGEAAFEEVARETELPCLTLLPANRDLVGAEVELVGA
                     ERREFRLKERLAGVKDRFEYVLVDCPPSLGFLTLNALAAADGLLLPIQCEYYALEGVS
                     ELLATMERVKESLNPALEVAGVVPTMWDERTNLSRQVLEEIRKHFGDRVFRTPVPRSV
                     RLAEAPSFGKPILLYDARSRGAEAYLAIAREMLRRDAR"
     gene            complement(8167..>8728)
                     /locus_tag="PLB01_12425"
     CDS             complement(8167..>8728)
                     /locus_tag="PLB01_12425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ78151.1"
                     /translation="EAYLAFLLERNAEVNLVSARAAEPEALASSHLFDALFGLPFLPP
                     PGAGRRLLDLGSGGGFPAVPLLLVRRDLAGTLVESVGKKCAFLREAGERLSLTLGVVN
                     ARFPDSFPMAKSGPFDVLTTRAVGSAGKLVSAARPVLAPGARALLWTTEPLVKDAVRD
                     SRAKSSAFHRDPLSERRGLLVLERFT"
BASE COUNT         1210 a         3004 c         3167 g         1347 t
ORIGIN      
        1 gagagctcgt cgatgccgag gatcgcgatg atgtcctgga ggtccttgta cttctggagg
       61 acctgctgga cggcgcgcgc gaccttgtag tgctcctcgc cgaccgtgag cggcgaaagg
      121 atcttcgacg tggaggcgag cgggtcgacg gacgggtaga tcccgatctc ggcgatctgg
      181 cgcgagagga cggtcgtcgc gtcgaggtgt gcgaacgcgg tcgcgggcgc cgggtccgtg
      241 aggtcgtcgg ccgggacgta aatggcctgt accgacgtga tcgagccgcg cttcgtggac
      301 gtgatgcgct cctggagctc acccatctcg gtcgcgaggt tcggctggta gccgacggcc
      361 gagggcatgc gcccgaggag cgccgagacc tcggagcccg cctgcgtgaa ccggaagatg
      421 ttgtcgatga agaggaggac gtccttgcct tccttgtcgc ggaagtactc ggcagccgtg
      481 aggcccgtca acccgacgcg caggcgcgcg ccgggaggct ccgtcatctg gccgtagatg
      541 agagcggcct tggacttctt ccagtccttc ggatcgatga ttccggactc ggtgaactcg
      601 agccagaggt cgttgccctc gcgcgtgcgc tcgccgacgc ccgcgaagac cgagtagccg
      661 cccgcggcct tcgcgacgtt gttgatgagc tccatgatga gaacggtctt gccgacgccc
      721 gcgcccccga agaggcccgt cttgccgccg cgcgtgtagg gctcgaggag gtcgatgacc
      781 ttgatgcccg tctcgaacat ctcgacggac gtggactggt cctcgtaggc cggcgcctcg
      841 cggtggatcg gccagtgctc ggtcgtcgtg acggggccca tctcgtcgac gggctcgccg
      901 atgacgttca ggacgcggcc gagcgtctcg gggccgacgg ggatcgtgat gggcgccccg
      961 gtgtcgatcg ccttcatgcc gcggacgagg ccgtcggccg gcttcatcga gatgcagcgg
     1021 acgcggttct cgccgaggtg ctgcgcgacc tcggtgatga tgtcgatggg gaccgtggag
     1081 agcttttcgt cgtccacgat gcggacggcg ttgaggatgg tcgggagctt cccggccggg
     1141 aactccacgt cgacaacggg tccgatgacc tggacgaccc tgccttcagt agccataggt
     1201 gcgcagcctt tccgtgtccc gcggcctagt cgagcgccgc ggctcccgag acgatctcga
     1261 tgagctcttt cgtgatgcgc gcctggcgcg cgcggttgta cgtgagggtg aggccgtcga
     1321 tgatctcgcc cgcgttcttg gaggcgctgt ccatcgcggt catctgcgcg gcgaagaacg
     1381 ccgcgttcga ctcgagcagg acgcggtaca tctggaactc gaggaagcgg ggcacgagcg
     1441 tgccgaggat gacgtccggc ccgggctcgt agaggtagtc cgtgccgccg gcgccggggg
     1501 cggccgcccc ggcctcgaga ctcaccggga ggagcttctt cgtccggacg atctgggaga
     1561 tcacgctccg gaactcgttg tagacgacat agacggcgtc gacctcgccc gcgacgaagc
     1621 gcgcgccgag gtcgcgggcg atctcgctcg cgaccgccat gttcagcttc gagaagaggc
     1681 cgggccgcgc gtcgaggatc tcgaccggcc gccgcttcca gaagtcgttc gacttcttgc
     1741 cgagggcgat gagccgaacc gagacgccct tctccttctc ctcgcgcagg aaggcgccca
     1801 cggcccggtt gacgttcgtg ttgaagccgc cggcgaggcc cttgtcgccg ctgacggcga
     1861 cgacgacgac gtttttctcc tcgcgcccct ggaggagcgg gtgcgccggc gtgccgtctt
     1921 cgttcgccgg gacgcgcgcc gccacggagg cgagggtcgc ctcgagcgtc gtcgcgtacg
     1981 gccgcgccga gagcacccgg tcctgcgcgc ggcgcagctt cgacgcggcg accatcttca
     2041 tcgccttcgt gatctgctgc gtgttcttca cgctgcggat ccggcgccgg atgtcgatca
     2101 ggttggccat ggacgcgccg cgcggctccc tacgcgacct tcgcttcggg ccgcgcggcg
     2161 aggaagacct tcttcgcctc ggcgaccgcc gccgagagcg cctcctcgat ctccttcgtg
     2221 agcttgcccg cgacgcggat ctcgtcgagg agggacttct tctggtcctt caggtactcg
     2281 tggagcgacg tctcgaacgg gaggacggcc tcgacgagca ggtcgtcgag gaagcccttc
     2341 gtcccggcga agaccgacga cacctgcagg ccgacgtcca tcgggacgta ctggttctgc
     2401 ttgaggatct cgacgaggcg ccggccgcgg ttgagctggg cctgcgtcgt cttgtcgagg
     2461 tcggagccga actgggcgaa cgccgcgagc tcgcggaact gcgcgagatc caggcgcagc
     2521 gtgcccgaga tcgcgcgcat gaccttgatc tgcgccgacc cgccgacgcg cgacaccgag
     2581 atgccgacgt tgacggccgg acgctggccc gagaagaaga ggtccgactc gaggaagatc
     2641 tggccgtccg tgatcgagat gacgttcgtc gggatgtagg ccgagacgtc gcccgcctgc
     2701 gtctcgatga tcggcagcgc cgtcagcgag ccgccgccct tctccttgct gagcttgctc
     2761 gcgcgctcga ggagccggct gtggaggtag aagacgtcgc cggggtacgc ctcgcggccc
     2821 gggggccggc gcaggagcag cgagatctcg cggtaggacg ccgcctgctt cgagaggtcg
     2881 tcgtagatga cgagcgcgtg gcggccgttg tacatgaagt actcgcccat cgcgcagccc
     2941 gcgtacgcgg cgatgtactg gaggggcgcc gggtcggacg ccgacgccgc gacgatgatc
     3001 gtgtggtcca tcgcgccgtg cttctcgagc gtctggacga cctgcgcgac cgtcgagttc
     3061 ttctgcccga tcgcgacgta gacgcagatg acgtccttgc ccttctggtt gatgatcgcg
     3121 tcgagcgcga tggcggtctt gccggtctgg cggtcgccga tgatgagctc gcgctggccg
     3181 cggccgatcg ggatcatcgc gtcgatcgcc ttgatgccgg tctgcatcgg ctccttgaca
     3241 ggcatgcgat cgacgacgcc gggcgcgagg cgctcgacgg ggtagaactc cttcgaggcg
     3301 atcgggccct tgccgtccag cgggatgccg agcgggttca cgacgcggcc gacgagggcg
     3361 tcgccgaccg gcacggacac gatgcgcttc gtgcgcttgg ccgtgtcgcc ttccttgacg
     3421 aggctcgtct cgcccatgag cacgcagccg acgtgcgact cctcgaggtt cagcgcgagg
     3481 ccgaacacct cgttcgggaa cgcgatgagc tcgccggcca tcgccttgtc gagcccgtag
     3541 acgagcgcga tgccgtcgcc gaccgagatg accgtgccga cctcgtcgac gtcgacgccc
     3601 tgcgtgacgc ccgcgagctg ggccttgatg agctgggtga tttcctgggc ggtgatgtcg
     3661 gccataagct tctttctccg gtcaagacgc cggggcgagt gcgtgcagcg cggcgcgagc
     3721 cttctcgagc cggcgcgcga gcgacgcgtc gaggcgggcg ctgcccaccg agacgacgaa
     3781 gccgccgagg agcgaggggt cgacggacgg gacgacgcgc acggtcgacc cggtgcggcc
     3841 ctcgaccatg cggcgcaggg cctcgaagac cgcatcgtcg agcgggcgcg cgctcgtgac
     3901 gtgcgcctcg agggtgcggc gctcgcggtc cagccgctcg cgcacggccg cgaggaacgc
     3961 cgggaggcgg tgcacgcgtc ggttctggag gaggatgtcg aggagacggc ccccgagcgc
     4021 gtcgacgccg accttcgcgg cgacggcgtc gagaagcgcc ttgcggcgcg tccgctcgac
     4081 gccggggttc gcgaggacct tcgagagctc cgtgttcgac tcgatcgcgt gggcgaccgc
     4141 gtcgagcgcc ggaagctgcg cgtcgacggc ctcggcggag cccgcgacgg cgaagaacgc
     4201 gtcgacatag gggcgcgtga atcgttcggc catcgtcagc ccttcgtccg cgccgaaagc
     4261 tcggccacgc cctcggcgac gaggcgcttc tcgtcctcgg gcgtgacgtt cttcgcgagg
     4321 atctcgcggg cgacctcgac ggcgaggtcg cccgcgtagg cggtcagctc ggcgcgcgcg
     4381 gcgcgcacgc ggttgtccac gtcggtcgag gcgcgcgcga ggaaccgcgc cgcgtcttcc
     4441 tcggtctgct tgagaagggc cgcgtgctcg gcctcggcgt cgcgccgcgc gctctcccgg
     4501 aggttcgcaa gctcggtttc gatctgcgcg agccggctgg cgagctcggc cgcgagcgcc
     4561 tcggcgcgct tccggtcgtc ctcggccttc gcgagcgtct tggcgacctc ggtccggcgg
     4621 gtgccgaaga actccgcgac gggcttgcgc aggaaatacc agagaagccc gagaaacgca
     4681 gcgaggttcg tgacctgcca gaagaggttc ggaacgccga ggaaaccctt cgcggcttcg
     4741 tgcccttcct gcgccaggcc gagcagaatc gaggagatca cgccagcctc cgcccgagcg
     4801 cgagcgaggc gatctcgccc gccgtcgtgc ggacgttctt ggcgagctct gcgcgcgccg
     4861 cggcgatctc ggcgtcgagc ttggcctggg ccgcagccgc ggccagccgg gccttctcgc
     4921 gggcctcgtc gagaagcgct tgtcgcttcg cgttcgccgc gttccgggaa gcctgccgcg
     4981 cggccacggc ctcgcgacgg gtctcggtca gacggcggtc gatcgaggcg agcgtctctt
     5041 tctcgttctc gagcgctttc gcgagtgcgt ccgtcgccgt tgcggccttc ttctcgcgct
     5101 cctcgaggat cgacccgagc ggcttgaaga cccacgcgcg cagaacggcg tacgtggccc
     5161 agaagaggac catgatgatc agcagggaga gatcgggaag ctgcatgtcg gtccgtcgag
     5221 cgtcgtcgcc tgcgcgggcc gggacctcga ggaacggcct cgaaggaggc ggggcaacgg
     5281 cccggaagcg cgcgctggcc ggcgcgttat ctcacagcgg cggcatcggg tcaaacctcg
     5341 ggcgcgccgg ccgcggccct tcccgcgctt tcccccgcct gccatgattc cgaccccagg
     5401 ggccatgcgc gcgaaacaag aattaatcga tcgttttgta acgacgctcg agggagccga
     5461 ccggctctgg gacgagaaga tcgccgtcct gcggccgggc gagatcgcct gtcggccggg
     5521 ctgcttcgga tgctgcgtcg gactcttcgc gatcggcctg ccggaagcac tcgcgctgcg
     5581 ccacgcggtg tccgggctcc ccgcggcggc tcgcgcggcg gtcctcgccc gggcgacgcg
     5641 cgcggtcgaa cagagcgccg cgacgtttcc cggcgatgct gcggcgggtc tcctcgatcc
     5701 ggagagaagc gaggcggccg aggaggcgtg gttcctcgac gcgcgcggaa caccctgccc
     5761 cgcgctcgag ctcccttccg gccggtgcct ggcgtacgcc gcgcgcccca cgacctgccg
     5821 cacgtacggt ctcgccctcc gcgcgggcgg cgagacgctc gtctcggcct gcgaactgaa
     5881 cttccccgcg gcccccggcg ggcgcgtcca ttcgacagcg atcgcggcgg aggaccttac
     5941 cgccgtcgac caggcgctcg tcgaggccgc ggcggaggcc gggctccccg cgggcctcga
     6001 gacgacgatc gcgcacgcgc tcacgggcgc ggcgttcgac atcttgtagg cgggaacccg
     6061 tcacgcgggt tcccgcacct tccgctcggc gtgcttcgcc ggctccgctg gcgctcgccg
     6121 gcctcagccg cctgcgccct ccggggccgg tcgggcggtg cccgcctcga cggcgccctg
     6181 gatcacggcg ccctcttcga cgacgagcgc cggcgcgtgg acctcggcaa aaagccttcc
     6241 gcccgcgtgg acctcgatcc gctcccgggc gtgaatgacg ccgcggaccg tacccgacac
     6301 cgcgagacgc ccgacgcgga tctcgccctc gacgaggccc ccgtctccga ccacgagctc
     6361 ggcgtccgag acgatcgtgc ccttgaaggc gccgtcgatc cggaacacgt cgtcgaacgt
     6421 gagcgtgccc tcgagccgcg cgccgcggtc gaggaatccg ttgagggtgc cgccggactt
     6481 cttcgtgctc acgcctctcc cccgcccgaa agcttctcga agaggccgat gagctcctcc
     6541 tccgagtaga acgcaatgcg cacctcgccg ccgcgtttct tcctccggat ctcgaccttc
     6601 gtgccgaggg cgcgcgcgag ccggagttcc gcgtcgcgag tgtcggcgtc gaccgcgcgg
     6661 tgcttccttt tcttcgcgcc gccgcccttc gcgagcgccg tgacgcgcgc ctcgacggcg
     6721 cgcacgctca ggccgcggcg ggcgacctcc ttcgcgagcg cctcctggtc cgcagcggac
     6781 gacagcgcga ggagcgcgcg cgcgtgcccc gccgtgagcg cgcccgactc gagcgcctcg
     6841 cgcaccgcgg ccggaagctt caggagacgc agcgcattcg cgaccgtcgc gcggtccttg
     6901 ccgacgcgct cggcgacgtc ctcctgcgtg aggccgaagt cctcgcgcag gtgacggtag
     6961 gcttcggctt cctcgagcgc cgtgaggtcc cggcgctgga cgttctcgac gagggcgatc
     7021 aggaggtggt cgcggtccgc gacgccttcg cgcacaacga cgggaacctc ggacaggccc
     7081 gcgaggcgcg ccgcgcggac ccgccgttca cctgcgagga tccggtagcg ctccccttcg
     7141 cgcgtgacga ggaccggctg aaggatcccc gtcttcgcga tcgagcgcga gagctcggcg
     7201 atgccgtcgt cgtcgaagcg gcggcggggc tgccgcctgt tcgcgtcgag gagagcgagc
     7261 ggcaccgtgc gcaggccgcc ggcctcgcgg cctggcttcg ccgtgggaat cagggccgac
     7321 aggcctcggc cgagcgcggt ctttcgggac atcgccggaa tcgtaacagc cgcggcgcgg
     7381 aggcccggcc ctcaggacgt cagcgtgcgt cgcgccggag catctcgcgg gcgatggcga
     7441 ggtacgcctc ggccccccgc gagcgcgcgt cgtagaggag gatcggcttt ccgaaggacg
     7501 gcgcctcggc gagccgcacg ctgcgcggga ccggcgttcg gaacacgcgg tcgccgaagt
     7561 gcttccggat ctcctcgagg acctggcgcg agaggttcgt ccgctcgtcc cacatcgtcg
     7621 ggacgacgcc cgcgacctcg agggccgggt tgaggctctc cttcacgcgc tccatcgtcg
     7681 cgaggagctc ggaaacgccc tcgagcgcgt agtactcgca ctggatcgga agcaggagcc
     7741 cgtcggccgc cgcgagcgcg ttgagcgtga ggaacccgag cgaaggcggg cagtcgacga
     7801 ggacgtactc gaaccggtcc ttcacgccgg cgaggcgctc cttgagccgg aattcccgcc
     7861 gctcggcgcc cacgagctcg acctcggcgc cgacgaggtc gcggtttgcg ggcaggagcg
     7921 tcaggcaggg cagctcggtt tcccgcgcga cttcctcgaa cgcggcctcg ccgagcagcc
     7981 agtcgtacgt ggtgcgtgcg agctcggcct tgtcgacgcc caggccgccg gtcgcgttcc
     8041 cctgcgggtc gagatcgacg aggaggaccg gccgctccag cgcggcaagg gcggccgcga
     8101 ggctgacggc ggtcgtggtc ttccccactc cgcccttctg gttcgcgacg gcgagaacgc
     8161 gatgtttcac gtgaaacgct ccaggacgag gaggccgcgg cgctcgctca aggggtcgcg
     8221 gtggaacgcg gacgatttcg cgcggctgtc ccggacggcg tctttcacga gcggctcggt
     8281 cgtccagagg agcgcccggg cgccgggcgc gaggacgggc cgggcggcgg agacgagctt
     8341 ccccgccgag ccgacggcgc gggtcgtgag gacgtcgaag ggcccgcttt tcgccatcgg
     8401 aaaggagtcg gggaatctag cgttcacgac gccgagcgtc aacgacaggc gctcgccggc
     8461 ctcgcggaga aacgcgcact tcttcccaac cgattcgacg agcgtacccg cgaggtcgcg
     8521 ccgcacgagg aggagcggaa ccgccgggaa cccgccgccc gacccgagat cgagaaggcg
     8581 acgccccgcg ccgggcggcg gaaggaaggg aaggccgaaa agcgcgtcga aaaggtggga
     8641 ggacgccagg gcttccggct cggctgcgcg cgccgacacg agattcacct ccgcgtttct
     8701 ttcgaggagg aacgcgaggt acgcctcg
//
LOCUS       DAOMFY010000154         8667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2485_length_8667_cov_7.871342, whole genome shotgun sequence.
ACCESSION   DAOMFY010000154 DAOMFY010000000
VERSION     DAOMFY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2485_length_8667_cov_7.871342"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1659
                     /locus_tag="PLB01_12430"
     CDS             124..1659
                     /locus_tag="PLB01_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ78152.1"
                     /translation="MPGPRILVLFGLLLVAGGLLVAPAAALAPGALLAGLGGILILVA
                     SFRDAAGPAREAETGVRAAFGPALDALETLAVFTVDRDGRVGYVNRGAIRVLGKPARE
                     LEGRPAGVVAYSEAEDPEIVEALREVFESGRPTAARRLLLYAGGGPRVESVVTMTPVV
                     RYGKVVEVAVFRADTRGGAPLERHGRLLDSVPAGLLGVDGTGRIDAVNRRLADLLGRR
                     ADALEGLDVARTEVLPESVRELLRAHAVRAAGTTPLSAGEEECVLVTPEGASRPVHVL
                     VSARAGGGADAVFIDGASRRRLRTELEASRQALLAAREAAAEAIVTTTHDLRAKIARI
                     VEAARRSTDESSGPIQRSAASAELKATTEQLLARVEADAAEALTPEVFGSGSGRPTVL
                     LVEDNEENRELLAHMLRSRGADVLAVGTGREAVEAAARFRFRFVLLDLQMPEMDGFQV
                     LRRLRALPAGGSLPVVALTALTSDLVKERCEAEGMNDFVSKPVTLARVGELVAKWGSR
                     IDA"
     gene            complement(1667..2086)
                     /locus_tag="PLB01_12435"
     CDS             complement(1667..2086)
                     /locus_tag="PLB01_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HQQ78153.1"
                     /translation="MTVRDLPTLNAFLNATSAVLLVLGWTFIRRKNRDAHRRTMIAAF
                     VCSAVFLTSYLAYHAQVGSVRFPGTGGARTLYLGILLTHSVLAAAVAPMAVTTFVLAW
                     KGRFEKHRRLARWTLPIWLYVSVTGVVVYVMLYRMAW"
     gene            complement(2083..2862)
                     /locus_tag="PLB01_12440"
     CDS             complement(2083..2862)
                     /locus_tag="PLB01_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78154.1"
                     /translation="MSAGLALAKRELVRFLRQKSRVVGAVAPPLVMWLLVGSGFKGSF
                     RGDSFGYFFPGTVVLVVLFAAIFATISVIEDRREGFLQGVLVAPVAPASIAFGKILGG
                     SVLGLLQGLPLLVLVPVAGLSADPRGLVGAAAVLVLLAFALTSLGFAIAWSLESTQGF
                     HAIMNLFLIPMWLLSGSFFPVEGAPAWLRATMAVNPLTYGVAALRRALGGTSESLPSL
                     SLSFAVTAAFAVVMFAAATAVVARWPSGPERRRLKAGRIAP"
     gene            complement(2859..3836)
                     /locus_tag="PLB01_12445"
     CDS             complement(2859..3836)
                     /locus_tag="PLB01_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ78155.1"
                     /translation="MTAPAVETAALSCAYGARLALDGATLSVARGEIFALLGPNGGGK
                     TTLFRILATLLRATSGTFRIDGLDPSADVRAVRRRLGVVFQTPGLDRKLTVTENLALQ
                     GALLNRTGRALAGRIDELLDAFSIRDRAGDLVETLSGGLARRVEIAKALLAAPPVLLL
                     DEPSTGLDPGARRDLTALLSELAKGGTAVLLTTHLFDEADAAHRIGILDRGRLVALGA
                     PAVLKAEVGGDVVTVEAREEGGAPALAREVSERFGAEAGKVAHVGNTVRVERPEAHTF
                     VPSLVEAFPGRFRSLTLGAPSLEDVFIDRTGRAFREADAEEPAPAGGPA"
     gene            complement(3847..4725)
                     /gene="cyoE"
                     /locus_tag="PLB01_12450"
     CDS             complement(3847..4725)
                     /gene="cyoE"
                     /locus_tag="PLB01_12450"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQQ78156.1"
                     /translation="MHSLSRSLAAYVSLTKPRITVFVVMTAFVGFVAGTKGPLSGMDL
                     ALLLHTLLGTAFVASGTSAFNQVWEKELDGRMARTASRPLPSGLLSRTGAALFAGALS
                     AAGLLELWIFTDPVTTALAAFTLVSYVLVYTPMKTRSPASTIVGAVPGALPPLGGFTA
                     AAGGAVGLPGLALFAILFVWQLPHFFAIGWRHRDDYAKAGVVILPVVDPSGRSTARQT
                     LLWTAVLLPISLLPSLVGSAGFVYAVGAFAMTLLFLNASFKFAREVSDGRAKSLFLAS
                     IAWLPAVLTLLVADRL"
     gene            complement(4727..5650)
                     /locus_tag="PLB01_12455"
     CDS             complement(4727..5650)
                     /locus_tag="PLB01_12455"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQQ78157.1"
                     /translation="MPETFLRIGTRTVAVCVFLLLIAGALVTSTQSGLAVPDWPLSYG
                     KVMPPMVGGILYEHGHRLVAAAVSTLVGLEMATLFFFEGRKTVRLLGVIAFVAILAQA
                     LLGGLTVLFLLPPAVSSAHAALAEIVFALTAVVALMCSKGWENFRISLKGEEARSAFR
                     WVVLATAAVYLQIVVGAVMRHTGAGLAIPDFPTSFGGLLPTFEEISRPGVPVHLAHRL
                     GALAVVLLVFRAAKALAALSERLPLLATAGAAWAGLVCLQAFLGASSIWSAKAVPLTV
                     AHLAVGALCWVIGVLASVALAAGFSDKLARD"
     gene            complement(5643..6485)
                     /locus_tag="PLB01_12460"
     CDS             complement(5643..6485)
                     /locus_tag="PLB01_12460"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQQ78158.1"
                     /translation="MTGSTLRFRFAPLCAAVLVGAAALAGCGGKDEDEDRPKSSRAAG
                     ASKDAAAPAAASAAPAGPAAGATGAASVTGKIVFDGAVPAAEKFKMSADAFCAKAHPG
                     DVAREDVVVAADKGLANVFVYVKTGITGTYPPPATAAVIDQKGCTYHPHVFGVVTGQN
                     IEILNSDPTLHNIHSLPEKNEAFNLGMPVQGMKYTKKFDKPEVMVRIKCDVHGWMSAF
                     CGVVAHPFFAVTAADGTYMIKNLPAGTYTIEAWHEKFGTQTQQVTVGATDSKAVPFTF
                     KAGA"
     gene            6567..7673
                     /gene="menC"
                     /locus_tag="PLB01_12465"
     CDS             6567..7673
                     /gene="menC"
                     /locus_tag="PLB01_12465"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HQQ78159.1"
                     /translation="MRVESAVLRHVEMPLKFRFRTSFGETSVKKFLLLELRGEGLSGW
                     GECVAEEAPFYSPETLATALPILTTYLLPLVLGKDLASPPAFDDSARRVRGNRMAKAA
                     VECALRDLQARAAGVPLGKALGGTREVIEVGVSLGISPSARETVENVRKHVADGYRRI
                     KLKIEPGWDAEPLAAVREAFPDVTLTVDANAAYTLADADTLRSLDAFGLDYVEQPLHH
                     EDLVAHAELAKAMKTPICLDESIRSEADAKAALQLGACQVINVKIGRVGGHGEAVRIH
                     AAAREAGVPVWCGGMLEAGVGRAHNVAIASLPGFSKPGDTASSSRYFEEDIVEPALEA
                     ASGLMPVPPGPGTGVTVRQDVLARYTTAVTEFRA"
     gene            7670..>8667
                     /locus_tag="PLB01_12470"
     CDS             7670..>8667
                     /locus_tag="PLB01_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ78160.1"
                     /translation="MSRAGDLGSMFAARKERLLADLEALVVRESPSDESARVSQLALW
                     IAERLGRAGLAAACVPCEGRGDAVRVRFGEETGGTLLLGHLDTVWPAGTLAEIPFSVE
                     DGIAKGPGVFDMKSGIAVALAVLEAAAKGDVTPAGGVSLVLTPDEEVGSGASSGLLVE
                     EALRRDRVLVLEPSGDGGAAKVARKGTGLVTVRFSGVASHAGLEPEKGASALLEMARF
                     ALYADALADKPAGTSVVPTVAGSGKVTNVVPERAGLTVDFRVWTRTEADRLMAGLRAY
                     RPSDPRVTVEIDGGPNRPPMEPTEASLELYRRAASLAHALGFELPAARVGGGSDGNL"
BASE COUNT         1330 a         2997 c         3245 g         1095 t
ORIGIN      
        1 tcggcctcgt acggcgcgtg gtctcggccc gacagccgcg cgcgagcgcg cgcgaggagg
       61 tcggaccacg tggcggcgga ggactcgggt tcggtcattg ggtctcggct ctaaacttgt
      121 gccatgcccg gcccgaggat tctcgtcctt ttcggtctgc tgctcgtggc cgggggcctt
      181 ctggtcgcgc cggcggccgc cctcgcgccg ggggcgctgc tcgcaggcct gggcgggatc
      241 ctgatcctcg tcgcgtcgtt ccgggacgcc gcggggcccg cccgcgaggc cgagacgggc
      301 gtccgggccg cattcgggcc ggcgctcgac gcgctcgaga cgctcgccgt cttcaccgtc
      361 gaccgcgacg ggcgggtcgg gtacgtgaac cgcggggcca tccgcgtcct cgggaagccc
      421 gcgcgcgagc tcgaaggccg tcccgcggga gtcgtcgcgt actcggaggc cgaggacccg
      481 gagatcgtcg aggcgctgcg cgaagtcttc gagtcgggcc ggccgacggc tgcgcggcgg
      541 ctgctcctct acgccggcgg cggtccccgg gtggagtccg tcgtcacgat gacgccggtc
      601 gtgcgctacg gaaaggtcgt cgaggtcgcg gtcttcaggg ccgacacgcg gggaggcgca
      661 ccgctcgagc ggcacgggcg cctgctcgat tccgtcccgg cgggtctcct cggcgtggac
      721 ggcacgggcc gtatcgacgc ggtgaaccgc cggctcgcgg acctcctcgg acgtcgtgcg
      781 gacgccctcg agggcctcga cgtcgcgcgg accgaggttc tcccggagtc ggttcgcgag
      841 ctgctgcgcg cgcacgccgt gcgcgccgcg ggcacgacgc cgctctcggc cggcgaggag
      901 gagtgcgttc tcgtcacccc cgagggcgcc tcgcggccgg ttcacgtcct cgtctcggcg
      961 cgggccggag gcggggccga cgccgtgttc atcgacggcg cctcgcggcg ccgcctccgg
     1021 accgagctcg aggcctcgcg ccaggcgctc ctcgcggcgc gggaggccgc ggcggaagcg
     1081 atcgtgacga cgacgcacga cctgagggcg aagatcgccc gcatcgtcga ggccgcgcgg
     1141 cgctcgacgg acgagagctc ggggcccatc cagcgcagcg cggcctcggc cgagctgaag
     1201 gccacgacgg aacagctgct tgcccgcgtc gaggccgacg ccgcggaggc gctgacgccc
     1261 gaggtcttcg ggagcggcag cgggcgcccg acggttctcc tcgtcgagga caacgaagag
     1321 aaccgcgagc tcctcgcgca catgctgagg tcgcgcggcg ccgacgtcct cgccgtgggc
     1381 accggccgcg aggccgtcga ggccgcggcg cgcttccggt tccgcttcgt cctcctcgac
     1441 ctccagatgc cggagatgga cggcttccag gtgctccggc gcctccgcgc cctcccggcc
     1501 ggcgggagcc tgcccgtcgt cgcccttacc gccctcacct ccgacctcgt gaaggagcgc
     1561 tgcgaggcgg agggaatgaa cgacttcgtg tcgaagcccg tcacgctcgc gcgcgtcggc
     1621 gagctcgtcg cgaagtgggg gagccggatc gacgcgtgac gcggggctac caggccatcc
     1681 ggtacagcat cacgtagacg acgacgcccg tcacggagac gtagagccag atcgggagcg
     1741 tccatcgggc gaggcgccgg tgcttctcga agcggccttt ccacgcgagg acgaacgtcg
     1801 tcacggccat cggcgcgacg gcggccgcga ggaccgagtg cgtaaggagg atgcccaggt
     1861 acagcgtcct cgcgcccccc gtgcccggaa accgcacgct cccgacctgc gcgtggtagg
     1921 cgagatacga cgtcaggaag acggccgagc agacgaacgc ggcgatcatc gtgcggcggt
     1981 gcgcgtcacg gttcttccgg cggatgaacg tccagccgag gacgaggagc acggcgctcg
     2041 tcgcgttcag gaaggcgttc agggtcggaa ggtcgcggac ggtcatggcg caattctgcc
     2101 cgctttgagg cggcgccgct cggggccgga cggccacctc gcgaccacgg ccgtcgcggc
     2161 ggcgaacatc acgacggcga acgccgcggt caccgcgaaa gagagactca gcgagggcaa
     2221 ggactccgac gttccgccga gcgcgcggcg gagggctgcg acgccgtacg tcagcgggtt
     2281 gaccgccatc gtcgcgcgga gccacgcggg cgctccctcc accgggaaga acgacccgga
     2341 gaggagccac atggggatca ggaagagatt catgatcgcg tggaagccct gcgtcgactc
     2401 gagggaccac gcgatcgcaa agccgaggga cgtcagcgcg aaggcgagga ggacgaggac
     2461 cgccgcggcg ccgacgaggc cgcgcgggtc ggccgaaagg cccgccacgg ggacgagaac
     2521 gaggagcggg aggccctgga ggagcccgag gacgctgccg ccgaggatct tgccgaacgc
     2581 gatcgacgcc ggcgcgacgg gcgcgacgag gacgccctgc aggaacccct cgcggcggtc
     2641 ctcgatgacg gagatcgtcg cgaagatcgc cgcaaagagg acgacgagga cgaccgtccc
     2701 ggggaagaag tagccgaagg agtctccgcg gaacgagccc ttgaagccgg agccgacgag
     2761 cagccacatg acgagcggcg gcgcgaccgc gccgacgacg cggctcttct gcctcaggaa
     2821 ccggacgagc tcgcgtttcg cgagggcgag gcctgcgctc acgccggccc tcccgcaggc
     2881 gcgggctcct cggcgtccgc ctcgcggaac gcacggccgg tccggtcgat gaagacgtcc
     2941 tccagcgagg gcgcgcccag cgtgagcgac cggaaccggc ccgggaacgc ctcgacgagc
     3001 gagggcacga acgtgtgggc ctcggggcgc tcgacgcgga cggtgttccc cacgtgcgcg
     3061 accttgccgg cctccgcgcc gaagcgctcc gagacctcgc gcgcgagcgc gggcgcgccg
     3121 ccctcctcgc gcgcctcgac cgtgacgacg tccccgccga cctcggcctt gagcacggcg
     3181 ggcgcgccga gggcgacgag gcgaccgcgg tcgaggatcc cgatgcggtg cgcggcgtcg
     3241 gcttcgtcga agaggtgcgt cgtgaggagg acggccgtgc cgcccttcgc gagctccgag
     3301 aggagcgccg tgagatcgcg gcgcgcgccg gggtcgagcc ccgtggaggg ctcgtcgagg
     3361 agcaggaccg gcggcgctgc cagaagcgcc ttggcaatct cgacgcggcg ggcgaggccg
     3421 cccgagagcg tctcgacgag gtccccggcg cggtcgcgga tcgagaacgc gtcgaggagc
     3481 tcgtcgatcc ggccggcgag ggcgcggccc gtcctgttca ggagcgcccc ctgcagcgcg
     3541 aggttctccg tcacggtgag cttccggtcg aggccgggcg tctggaagac gacgccgagc
     3601 ctgcggcgca ccgccctgac gtcggccgag ggatcgagcc cgtcgatgcg gaaggtcccc
     3661 gacgtggccc tgaggagcgt cgcaaggatg cggaagagcg tcgtcttgcc gccgccgttc
     3721 gggccgagga gcgcgaagat ctcgccgcgc gccaccgaga gcgtcgcgcc gtccagcgcg
     3781 aggcgcgcgc cgtacgcgca cgagagcgcg gcggtctcga cggcgggagc ggtcacgcgc
     3841 ggcgcgtcag agccggtcgg cgacgaggag cgtgagcacg gccgggagcc acgcgatcga
     3901 agcgaggaac agcgacttcg cccggccgtc cgagacctcg cgggcgaact tgaacgacgc
     3961 gttgaggaac agcagcgtca tcgcgaacgc cccgacggcg tagacgaagc ccgcggagcc
     4021 gacgagcgac gggaggaggc tgatcgggag gaggacggcg gtccacagga gagtctggcg
     4081 cgccgtggag cggccggacg ggtcgacgac cgggaggatg acgacgccgg ccttcgcgta
     4141 gtcgtcgcga tggcgccagc cgatcgcgaa gaagtgcggg agctgccaca cgaagaggat
     4201 cgcgaagagc gcgagcccgg gaaggccgac ggcgccgccc gccgcggccg tgaagccgcc
     4261 gagcgggggg agcgcccccg ggacggcgcc gacgatcgtc gaggccggcg agcgcgtctt
     4321 catgggcgtg tagacgagaa cgtacgagac gagcgtgaac gccgccagcg ccgtcgtcac
     4381 ggggtccgtg aagatccaga gctcgaggag cccggcggcg gacagcgccc cggcgaagag
     4441 cgcggcgccg gtccgggaga ggaggcccga ggggagcgga cgcgaggccg tgcgcgccat
     4501 ccgtccgtcc agctccttct cccagacctg gttgaacgcg ctcgtgcccg acgcgacgaa
     4561 cgccgtgccg aggagcgtgt gcaggagcag cgcgaggtcc attccggaca ggggcccctt
     4621 cgtgccggcc acgaatccga cgaaggccgt catcacgacg aagaccgtga tgcgcggctt
     4681 cgtcagggac acgtaggcgg cgagggagcg gctcagagaa tgcacctcaa tcgcgcgcga
     4741 gcttatccga aaaaccggcc gcgagcgcga cggatgccag aacgccgatc acccagcaga
     4801 gggcccccac ggcgaggtgg gcgaccgtca gggggacggc cttggcggac cagatcgagg
     4861 aggccccgag gaacgcctgc aggcagacga ggccggccca ggcggcgccg gccgttgcaa
     4921 ggagcgggag acgctccgag agcgcggcga gggccttcgc ggcgcggaac acgagcagga
     4981 cgaccgccaa agccccgagg cgatgggcga gatgaacggg aacgccgggc cgggagatct
     5041 cttcgaaagt ggggagcagt ccgccgaacg aggtgggaaa gtccgggatc gcgaggcccg
     5101 cgccggtgtg ccgcatgacg gcccccacga cgatctgcag atacacagcc gcggtcgcca
     5161 ggacgaccca tcggaaggcg cttctcgcct cttcaccttt caaagaaatc cgaaaattct
     5221 cccagccctt cgagcacatg agcgcgacca cggcggtgag cgcgaagacg atctcggcga
     5281 gcgccgcgtg cgcggaagaa actgcgggag gaaggagaaa aagaaccgtc agacccccca
     5341 acagggcctg cgcgaggatc gcgacgaagg caatcacgcc gagcagccga accgtctttc
     5401 taccttcaaa gaaaaacagc gtggccatct cgaggcctac caatgtagag accgccgccg
     5461 ccacgagccg gtggccgtgc tcgtagagga tgccgccgac catcggcggc atcactttcc
     5521 cgtacgagag cggccagtcc gggaccgcga gcccggactg ggtggacgtg acgagggcgc
     5581 cggcgatcag gagcaggaag acgcagactg cgacggtccg ggtcccgatc ctgagaaacg
     5641 tctcaggcac cggccttgaa cgtgaacggc accgccttgg agtccgtcgc gcccaccgtg
     5701 acctgctgcg tctgcgtgcc gaacttctcg tgccacgcct cgatcgtgta cgtgcccgcg
     5761 ggcaggttct tgatcatgta cgtgccgtcg gcggccgtca ccgcgaagaa cgggtgcgcg
     5821 acgacgccgc agaacgccga catccagccg tggacgtcgc acttgatccg gaccatgacc
     5881 tcgggcttgt cgaacttctt cgtgtacttc atcccctgca cgggcatccc gaggttgaac
     5941 gcctcgttct tttcgggcag cgagtggatg ttgtggagcg tggggtcgct gttcaggatc
     6001 tcgatgttct ggcccgtgac gacgccgaag acgtgcgggt gatacgtgca gcccttctgg
     6061 tcgatcacgg cggcggtcgc gggcggtgga tacgtgcccg tgatgcccgt cttcacgtag
     6121 acgaagacgt tcgcgagacc cttgtcggcc gcgacgacga cgtcctcgcg ggcgacgtcg
     6181 cccggatgcg ccttggcgca gaacgcgtcg gcggacatct tgaacttctc ggccgccggg
     6241 acggcgccgt cgaagacgat cttgcccgtg acggaggcgg cgcccgttgc gcccgcggcc
     6301 ggtccggccg gggccgcgga ggcggcggcg ggagccgcgg cgtccttcga ggcgccggcg
     6361 gcgcgagagg acttcgggcg gtcctcgtcc tcgtccttcc cgccgcagcc cgcgagggcg
     6421 gcggcgccga cgagcacggc ggcgcagagg ggggcgaaac ggaaacgaag cgtggaaccg
     6481 gtcatgcggt cctccttcgg ggggtggagt ttctttgagg cagatccctt ctcggcgaag
     6541 ggaaacgaat gatagacaat cgcttcatgc gcgtcgaatc cgcggtcctc cggcacgtcg
     6601 agatgccgct caagttccgg ttccgcactt cattcggcga gacgtctgtc aagaagttcc
     6661 tcctcctcga gctgcgcggg gagggcctct cgggctgggg ggagtgcgtc gccgaggagg
     6721 ccccgttcta ctcgccggag acgctcgcga ccgcgctgcc catcctgacg acctatctcc
     6781 tccccctcgt cctcggcaag gacctcgcat cgccgccggc cttcgacgac tccgcccggc
     6841 gcgttcgcgg gaaccggatg gcgaaagccg ccgtagagtg tgctctgcgg gatctgcagg
     6901 cgcgggccgc gggcgtcccg ctcgggaagg ccctcggcgg aacgcgcgag gtgatcgagg
     6961 tcggcgtctc gctcgggatc tcgccgtccg cgcgggagac cgtcgaaaac gtgcggaagc
     7021 acgtcgccga cggctaccgg aggatcaaac tcaagatcga gcccgggtgg gatgcggagc
     7081 ctctcgcggc cgtgcgcgag gccttccccg acgtcacgct caccgtggac gccaacgcgg
     7141 cgtacacgct tgccgacgcg gacacccttc ggtcgctcga cgcgttcggc ctcgactacg
     7201 tcgagcagcc gctccaccac gaggacctcg tcgcccacgc ggagctcgcg aaggccatga
     7261 agacgccgat ctgtctcgac gagtcgatcc gctccgaggc cgacgccaag gcggcgctcc
     7321 agctcggcgc gtgccaggtc atcaacgtca agatcggccg cgtcggcggc cacggcgaag
     7381 ccgtgcgcat ccacgccgcg gcgcgcgagg cgggagttcc cgtctggtgc ggcggcatgc
     7441 tcgaggcggg ggtcggacgc gcgcacaacg tcgcgatcgc gagcctcccg ggcttctcga
     7501 agcccggcga cacggcgtcc tcgtcacgct acttcgagga ggacatcgtg gagccggcgc
     7561 tcgaggccgc gagcggtctc atgccggtcc cgccgggacc cggcaccggc gtcacggtga
     7621 ggcaggacgt tctcgcgcgg tacacgacgg ccgtcacgga gttccgcgcg tgagccgcgc
     7681 gggcgacctc ggctcgatgt tcgccgcgcg gaaggagcgc ctcctcgccg acctcgaggc
     7741 gctcgtcgtg agggagagcc cgtcggacga gtcggcccgt gtgtcgcagc tcgccctctg
     7801 gatcgccgag cgcctcgggc gcgcgggcct cgcggccgca tgcgtcccgt gcgagggccg
     7861 tggcgacgcg gtgcgcgtcc gtttcggcga ggagaccggc ggcacgctgc tcctcgggca
     7921 cctcgatacc gtctggcccg cgggcacact cgccgagatc ccgttctcgg tcgaggacgg
     7981 catcgcgaag ggccccggcg tcttcgacat gaaatccgga atcgcggtcg cgctcgcggt
     8041 cctcgaagcg gccgcgaagg gcgacgtgac gccggcgggc ggcgtctccc tcgtcctgac
     8101 gccggacgag gaggtcggca gcggcgcctc gagtggcctg ctcgtcgagg aagcgctccg
     8161 gcgcgaccgc gtcctcgtcc tcgagccctc gggggacggc ggcgccgcaa aggtcgcgcg
     8221 caaggggacc gggctcgtga ccgtgcgttt ttccggcgtc gcctcgcacg ctgggctcga
     8281 gcccgaaaag ggcgcctcgg cgctcctcga gatggcgcgc ttcgccctct acgccgacgc
     8341 cctcgcggac aagccggccg gcacgtccgt cgtcccgacc gtcgccggtt cgggcaaggt
     8401 cacgaacgtc gtgcccgagc gggccgggct cacggtggac ttcagggtct ggacgcgaac
     8461 ggaggccgac cggctcatgg cggggctccg cgcctaccga ccgtccgacc cgcgcgtcac
     8521 ggtcgagatc gacggcggcc cgaaccggcc gccgatggag cccaccgagg cgtcgctcga
     8581 gctctaccgg cgcgcggcct ccctcgcgca cgcgctcggc ttcgagctgc ccgcggctcg
     8641 cgtcggcggc ggctcggacg gcaacct
//
LOCUS       DAOMFY010000155         8604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2505_length_8604_cov_9.191718, whole genome shotgun sequence.
ACCESSION   DAOMFY010000155 DAOMFY010000000
VERSION     DAOMFY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2505_length_8604_cov_9.191718"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLB01_12475"
     CDS             complement(<1..573)
                     /locus_tag="PLB01_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ78161.1"
                     /translation="MNTTTTQRLQPDLVVLTQAAPVPGRGFLPIASYLLLGKQPVLVD
                     TGAARHRAGFVEALESVLDPKDLAWIVVSHADEDHTGALELLLDRAPKARIAMNWLST
                     GKLSAHFDPPMPRVTWVNPGEALVAGDRVLHALRPPMYDCPSTTTFFDSKSRALFTSD
                     AFGAFVPAMAETFADVDEKAVLEGMSVFCRA"
     gene            705..1181
                     /locus_tag="PLB01_12480"
     CDS             705..1181
                     /locus_tag="PLB01_12480"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQQ78162.1"
                     /translation="MRSILENLPRILAGHDAIAVGTRDAAMRPACSMAVGVAFPGEGR
                     VTVYVPEATGARTLADLEANGAIAVVFEEIPTHHTVQLKGRVTEIRPAGERERELVER
                     SIGGFFAQVEAVGGPPAVVRRKRRWPCRAVTFAVADVFEQTPGPKAGTPFVADGAR"
     gene            1178..2545
                     /locus_tag="PLB01_12485"
     CDS             1178..2545
                     /locus_tag="PLB01_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQQ78163.1"
                     /translation="MTPRLPDLMRCFQGIIPTALATCSADGVPNVTYISQLHYVGPTQ
                     LAISCQFFNKTKRNLEDNPYASVEFWDPVTFRAYRADLRYVRSETSGALFETMAHRIE
                     VIGSHTGMAGVFKLRSADVCDVLALSEVEGFLDENADLPEEPITDGPMTEFRALSLVS
                     GRINRAHDLESLYRDVLDALEEVFRFRHSIVFLADEKAETLTAIASHGYGESGVGAEV
                     PMGAGLVGYVAAKRVPIRLSGMDQLLRYGRAVRGRVQVAGDASGLSAEIPLPGLPDAE
                     SQLALPLVVEDRLLGVLAIESRNPLACAEWHEAFLSVLANQIAIGIDRLSEADEEPAT
                     AEPGPAPAPAAGPSRAFVYFRNDDCVFVDGEYLIRNVPAKILWKLLGEHVRTGRREFT
                     NRELRLDPSLGLPAVKDNLESRLILLKKRLEERCFGVRIVPVRRGRFALEVAAAVTLE
                     EREKG"
     gene            complement(2594..2950)
                     /locus_tag="PLB01_12490"
     CDS             complement(2594..2950)
                     /locus_tag="PLB01_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78164.1"
                     /translation="MKKNAPRNRTMAAAIAGILAGSALLASPARGDDKPDASKTPAAG
                     QKDKASCGGKDGCGGKKTDAKDADKHACKGQNACKGHGGCKSGDNGCAGKNSCKGHGG
                     CATAAKPKPTPAPPAA"
     gene            complement(3026..3262)
                     /locus_tag="PLB01_12495"
     CDS             complement(3026..3262)
                     /locus_tag="PLB01_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQQ78165.1"
                     /translation="MLTCRELIEFLDDYWAGQLRPEEKARFDEHLAVCPSCVAYLAQY
                     GRAAELGRAAFVEDGPDDAPPALLDAILKARTGR"
     gene            complement(3256..3888)
                     /locus_tag="PLB01_12500"
     CDS             complement(3256..3888)
                     /locus_tag="PLB01_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ78166.1"
                     /translation="MPTIETVAPPSEDAALLARLREGSDDAFTDLVRANTGRLLAVAR
                     RMLRQEQEAQDAVQEAFVCAFKALPTFEGQCRISTWLHRIAVNACLMRLRRRERKPEE
                     SIDDFLPAYEADGHTVEKFRPWDDSAIERLEKEEARALVRAAIDRLPESFRTALILRD
                     IEEMSSEEAAAVLGITPNAVKIRVHRARQALRTLLDPHFGGIGENRRAAC"
     gene            complement(3929..4249)
                     /locus_tag="PLB01_12505"
     CDS             complement(3929..4249)
                     /locus_tag="PLB01_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78167.1"
                     /translation="MNIGRKSLLVATLAGVLAAPAVARAEDEKGGGKGDEGVPCWGVN
                     KCKGVGDCGAGGCRTTGCHGSNACRGQGFLRLDKETCLKIQGGRLTQAAAKKPAKKAE
                     SNKS"
     gene            complement(4279..5091)
                     /locus_tag="PLB01_12510"
     CDS             complement(4279..5091)
                     /locus_tag="PLB01_12510"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HQQ78168.1"
                     /translation="MNGLRALQRDVLQDLNGVRGREAAERAFDFSRPPAGGVEDRWHV
                     YAHGYVARLAEALEADYAAVRRVLGPGPFAALAERYVRAFPPRSYDLGRAGDLLPEHL
                     SVDPIAVALPFLPDLARLERAVSEAFVAPDAEPLTWEALLEERPETVAALPLVLTPGV
                     TLLPSRWPVVSLWKTRTEPDEEVEVELERNPETALVFRSGLSVTCARLDAKETAFVAA
                     LTDGAATLTMLEARLAPGGGAAEIASLLAVFRSLVERSVFSSVGSTATNRSA"
     gene            complement(5088..5933)
                     /locus_tag="PLB01_12515"
     CDS             complement(5088..5933)
                     /locus_tag="PLB01_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HQQ78169.1"
                     /translation="MNGARQLTGCGIGLRKEHHDRVLAEKPDVPFFEVISENFMVEGG
                     RPAHVLERVRRDYPVALHGVSMNLGSADPLDAAYLARLKALVDRIEPAVVSDHLCWTA
                     LAGQNSHDLLPLPFTEEAVRHVAAKIHRAQDVLGRRLLVENVSSYVTFRSSAMSEAEF
                     LAAVANEADCHLLLDVNNLWVNGRNHGFDASEALRSIPAGRVRQFHLAGHADYGDVVI
                     DTHDRPVRDEVWALYRQAVRRFGAQPTLLEWDANVPELEVLLEETRLASVVQRSWAVP
                     ERLAA"
     gene            complement(5982..7025)
                     /locus_tag="PLB01_12520"
     CDS             complement(5982..7025)
                     /locus_tag="PLB01_12520"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQQ78170.1"
                     /translation="MSRDGSLRRWLTALPAALPLLGGFVALLVARLLAAGTDAPQVVR
                     KDARLDALVPPGAAVEKVADGFTWLEGPLWDARAKGLLFSDIPANAIHLWRPGAGTTR
                     VLSPSGYTGREPFAGKEPGSNGLAFDAEGRLVLCAHGDRAIVRIEHDGKRSVLAGRFH
                     ASRLNSPNDLAFSANGDLYFTDPPFGLPGGFDDPGRELPFSGVYRLAPDGRLTLLTQE
                     LDAPNGLAFSPDGRTLYVSNASARRAVWMAFPVRSDGTLAAGRVFFDATSWTRPGTGN
                     PDGLKVDAAGNLFAAGPGGVHVFTKDGSHLGTIAFPVPVSNVAFGEDGRTLYVTASSA
                     VWRVRLAARGAGF"
     gene            complement(7022..>8604)
                     /locus_tag="PLB01_12525"
     CDS             complement(7022..>8604)
                     /locus_tag="PLB01_12525"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQQ78171.1"
                     /translation="AAAGPGDPPEGTPAATLDLASAEGAAAVNAVWRVADARPVETGF
                     RLAGPDGQPGPEPSRTWDIAPHAGAADFDDSAWETVAPAALGLRRGGGRLSFAWYRVR
                     FTLPDRVGGFAVAGSTVVFETSIDDYAEVWVDGELPRRAGQAGGSVVAGWNAGNRVVA
                     LRNAKPGRTVSIAVFGANGPLSNPPTNYVWMRFARLLFYAERSKEPFAVIPEEVNVEV
                     LRNDPSLDAIVPLNPKIFRVADNFLFTEGPVWDARSTSLLFSDPNRNRIYRYTPDAGA
                     AGRLEVFREKSGYAGADVAEYGQPGSNGLTLDREGRLTFDQHGNHAVVRLERDGALTV
                     LADRYEGKRLNSPNDLVVRSNGDVYFTDPPFGLPRFFEDPRKELPFSGIFGILGGRLT
                     LLTKELSGPNGIAFSPDERVLYVGNWDDKRKVVMRYPVRDDGTLGAGTVFFDMTSAKG
                     EDAIDGIKVDARGNLYVSGPGGLWILSPDGRHLGTITGPKHVHNMAWGDPDGRTLYLC
                     ARSSLYRIRLNVPGARPE"
BASE COUNT         1217 a         2896 c         3197 g         1294 t
ORIGIN      
        1 cgcgcggcag aagacggaca tgccctcgag gacggccttc tcgtccacat cggcaaacgt
       61 ctcggccatc gccggcacga aggctccgaa cgcgtcggac gtgaagagcg cgcggctctt
      121 cgagtcgaag aacgtcgtcg tggacgggca gtcgtacatc ggggggcgga gcgcgtggag
      181 gacgcggtcg ccggcgacga gcgcctcgcc ggggttcacc cacgtcacgc gcggcatcgg
      241 cgggtcgaag tgcgccgaga gctttcccgt cgagagccag ttcatcgcga tccgcgcctt
      301 cggggcgcga tcgaggagga gctcgagggc gcccgtgtgg tcctcgtccg cgtgcgagac
      361 gacgatccac gcgaggtcct tcgggtcgag aacgctctcg agcgcctcga cgaagccggc
      421 gcggtggcgc gccgcgccgg tgtcgacgag gaccggctgc tttccgagga gaaggtacga
      481 cgcgatcggc aggaagccgc ggcccggcac gggagcggcc tgcgtcagga cgacgagatc
      541 gggctggagg cgttgtgtgg tggtcgtgtt catgtcggtc tccttgtgac gccgcaaacg
      601 tgcagcggtc acggggaggt cgccacctaa gaagttgcta ggcgcgcctt gagaacttcc
      661 tacgcttcct aaacgtttcc taagcgctgc gataatcttt gagcgtgaga tcgattctcg
      721 aaaaccttcc gcgaatcctc gcgggacacg acgcgatcgc cgtcggaacg cgcgacgccg
      781 cgatgcggcc cgcgtgctcg atggcggtgg gcgtcgcgtt cccgggggag ggccgcgtga
      841 cggtctacgt cccggaggcg accggcgccc ggacgctcgc ggacctcgag gcgaacgggg
      901 cgatcgcggt cgtcttcgag gagatcccga cgcatcacac ggtgcagctg aagggtcgcg
      961 tgacggagat ccgcccggcc ggcgagcgcg agcgggagct cgtggagcgt tcgatcggcg
     1021 ggttcttcgc gcaggtggag gcggtgggcg ggcccccggc cgtcgtgcgg cgcaagagac
     1081 ggtggccctg ccgcgccgtc acgttcgccg tcgcggatgt cttcgagcag acgccggggc
     1141 cgaaggcggg cacgccgttc gtcgcggacg gcgcccgatg acgccccgat tgccggacct
     1201 catgcgctgc ttccagggaa tcatcccgac ggcgctcgcg acgtgctcgg cggacggcgt
     1261 cccgaacgtg acgtacatca gccagctcca ctacgtgggc ccgacccagc tcgcgatctc
     1321 gtgccagttc ttcaacaaga cgaagaggaa cctcgaggac aatccgtacg cctccgtcga
     1381 gttctgggac ccggtgacgt tccgcgcgta tcgcgccgac ctgcgctacg tgcgctccga
     1441 gacgtccggg gccctcttcg agacgatggc gcaccggatc gaggtcatcg gttcgcacac
     1501 cgggatggcg ggcgtcttca aactgcgctc ggcggacgtc tgcgacgtcc tcgcgctctc
     1561 ggaggtcgag gggttcctcg acgaaaacgc cgatctgccc gaggagccga tcacggacgg
     1621 cccgatgacc gagttccgcg cgctgtcgct cgtgtccgga cgcatcaaca gggcgcacga
     1681 tctcgagagt ctctacaggg acgtcctcga cgcgctcgag gaggtcttcc gcttccggca
     1741 ctcgatcgtc tttctcgcag acgagaaggc ggagacgctc acggcgatcg cgagccacgg
     1801 gtacggcgag agcggcgtcg gtgccgaggt tcccatgggc gccgggctcg tcgggtacgt
     1861 cgcggccaag cgcgtgccca tccggctgtc gggcatggac cagctcctgc gctacgggcg
     1921 ggccgtgcgc ggccgtgtgc aggtcgcggg cgacgcgtcc ggcctctccg ccgagattcc
     1981 cctgcccggc ctcccggacg ccgagagcca gctcgcgctc ccgctcgtcg tcgaggaccg
     2041 gctcctcggc gttctcgcga tcgagagccg caacccgctc gcgtgcgccg agtggcacga
     2101 ggccttcctc tccgtcctcg cgaaccagat cgcgatcggg atcgacaggc tctccgaggc
     2161 cgacgaggag cccgcgaccg ccgagcccgg cccggctccg gctccggcgg cgggcccgtc
     2221 gcgcgccttc gtctacttcc ggaacgacga ctgcgtcttc gtggacggcg agtacctcat
     2281 ccgcaacgtc cccgcgaaga tcctctggaa gctcctcggc gagcacgtca ggacggggcg
     2341 gcgcgagttc acgaaccgcg agctccgcct cgacccgtcg ctcggactgc cggcggtcaa
     2401 ggacaacctc gagagccggc tcatcctcct caagaagagg ctcgaggagc gatgcttcgg
     2461 cgtgcgcatc gtcccggtcc ggcgcggccg cttcgccctc gaagtcgcag cggccgtcac
     2521 gctcgaggag cgcgaaaaag gctgacggcc gccgcggatg cggcggccgc cagcagatcg
     2581 tcgagggccg gcctcaggcg gcggggggag ccggcgtcgg cttgggcttc gcggccgtgg
     2641 cgcagccgcc gtggcccttg cacgagttct tgcccgcgca gccgttgtcg ccgctcttgc
     2701 agccgccatg gcccttgcac gcgttctggc ccttgcaggc gtgcttgtcg gcgtccttgg
     2761 catccgtctt cttaccgccg cagccgtcct tgccgccgca ggacgccttg tccttctggc
     2821 cggcggcggg cgtcttcgag gcgtcgggct tgtcgtcgcc gcgcgccggc gaggcgagca
     2881 gggccgagcc ggcgaggata ccggcgattg cggcggccat ggtgcgattg cggggagcgt
     2941 tcttcttcat cgtttctcct ttgtgcgcgt tcttcaacgg gcgacgatga atgccgcggg
     3001 cgggacgaaa cattcccgcc ccggatcagc gacccgtgcg cgccttcagg atggcgtcga
     3061 gaagcgccgg cggtgcgtcg tcggggccgt cttcgacgaa tgcggcccgg ccgagttccg
     3121 cggcgcgacc gtactgcgcg aggtacgcca cgcagctcgg acaaaccgcg agatgctcgt
     3181 cgaaacgagc cttctcttcg ggacggagtt gtccggccca gtagtcgtcg aggaactcga
     3241 tgagctcgcg gcaggtcagc atgcggcgcg tcgattctct ccgattcctc cgaagtgcgg
     3301 gtcgaggagc gtccggagcg cctggcgggc gcggtggacg cggatcttga cggcgttcgg
     3361 cgtgatcccg aggacggcag cggcctcctc ggagctcatc tcctcgatgt cgcgcaggat
     3421 cagcgccgtg cggaagctct cggggaggcg gtcgatcgcg gcgcggacga gcgcgcgtgc
     3481 ctcctctttc tcgaggcgct cgatcgccga gtcgtcccac ggccggaact tctccacggt
     3541 gtgaccgtcg gcctcgtacg ccgggaggaa gtcgtcgatc gactcctcgg gcttgcgctc
     3601 gcgccggcgc aggcgcatga ggcacgcgtt cacggcgatg cggtgcagcc acgtcgagat
     3661 ccggcactgc ccctcgaagg tggggagcgc cttgaacgcg cagacgaacg cctcctgcac
     3721 cgcgtcctgg gcttcctgtt cctggcggag catgcggcgg gcgacggcga ggaggcggcc
     3781 cgtgttggcg cgaacgagat cggtgaaggc gtcgtcgctg ccttctcgca ggcgcgcgag
     3841 gagtgccgcg tcttcggagg gaggggcgac cgtctcgatc gtcggcatgt tctgccgatt
     3901 ggatgcggga cggccgcgcg tttattcctc agctcttgtt cgactcggcc ttcttggcgg
     3961 gcttcttggc ggcggcctgc gtcagtcggc cgccctggat cttgaggcag gtctccttgt
     4021 cgaggcgcag gaagccctgg ccccggcagg cgttcgagcc gtggcacccg gtcgtgcggc
     4081 agccgccggc gccgcagtct ccgacgccct tgcacttgtt gacgccccag cacgggaccc
     4141 cttcgtcccc tttgccgccc cccttctcgt cttcggcgcg ggcgacggcg ggcgccgcga
     4201 gaacgcccgc gagggtggcg acgagaagcg actttcttcc gatgttcatg aacacttcct
     4261 ttcaggttgc ggaggctttc aggcgctccg gttggtggcg gttgagccga cgctggaaaa
     4321 gacactacgc tcgacgagcg agcggaagac ggcgaggagg gatgcgatct cggccgctcc
     4381 gcccccgggc gcaaggcgcg cttcgagcat cgtgagcgtg gcggcgccgt ccgtcagggc
     4441 cgcgacgaag gcggtttcct tcgcgtcgag gcgtgcgcac gtcaccgaga gcccgctccg
     4501 gaagacgagc gccgtctcgg ggttcctctc gagctcgact tcgacctctt cgtcgggctc
     4561 ggtccgcgtc ttccagaggg acacgaccgg ccagcgcgac ggcaggagcg tcacgccggg
     4621 ggtgaggacg agcgggagcg cggcgacggt ctccgggcgt tcctcgagga gcgcctccca
     4681 ggtcagcggc tcggcgtcgg gggcgacgaa ggcctccgag acggcccgct cgaggcgcgc
     4741 gaggtccggg agaaagggga gcgccacggc gatcgggtcg acggacaggt gctcgggcag
     4801 gaggtccccc gcgcgtccga ggtcgtagga gcggggtggg aacgcacgca cgtaacgctc
     4861 ggcgagcgct gcgaacggcc ccggaccgag aacccgccgc acggcggcgt agtccgcttc
     4921 gagcgcctcg gcgaggcgcg cgacgtagcc gtgtgcgtac acgtgccagc ggtcctcgac
     4981 gccgcccgcg ggcggacggg agaagtcgaa cgcgcgctcg gcggcctcgc gcccgcggac
     5041 gccgttgagg tcctgcagga cgtcgcgctg gagcgcgcgc aggccgttca cgccgccagc
     5101 ctttcgggga cggcccagga gcgctggacg accgaggcca ggcgggtttc ctccagcagc
     5161 acctcgagct cggggacgtt cgcgtcccac tcgaggagcg tgggctgcgc gccgaagcgg
     5221 cggacggcct gccggtagag cgcccagacc tcgtcgcgca cgggacggtc gtgcgtgtcg
     5281 atgacgacgt ccccgtagtc cgcgtggccg gcgaggtgga actgccgcac ccggcctgcc
     5341 ggaatcgagc ggagcgcctc tgaggcgtcg aaaccgtggt tccggccgtt cacccacagg
     5401 ttgttcacgt ccagcaggag gtggcagtcc gcctcgttcg cgacggcggc gaggaactcg
     5461 gcctcgctca tcgcggacga gcggaaggtg acgtaggacg agacgttttc gacgaggaga
     5521 cgccggccga ggacgtcctg cgcccggtgg atcttcgcgg cgacgtgccg gacggcttcc
     5581 tccgtgaagg gcaggggcag gagatcgtgg ctgttctgtc ccgcgagcgc ggtccagcag
     5641 agatgatccg acacgacggc gggttcgatg cggtcgacga gcgccttgag gcgcgcgagg
     5701 tacgcggcgt cgagagggtc cgccgagccg aggttcatcg agacgccgtg cagcgcgacc
     5761 gggtagtccc gccgcacccg ctcgagaaca tgcgcgggcc ggccgccttc gaccatgaag
     5821 ttctcggaga tgacctcgaa gaacgggacg tccggtttct cggcgaggac gcggtcgtgg
     5881 tgctccttgc gaaggccgat cccgcaaccc gtcagctgtc gtgctccgtt catcgtgtcc
     5941 ttcctcgccg tcttggatgc ccgggccgcc gcggggtttc gtcagaagcc cgcgccgcgg
     6001 gccgcgagcc ggacgcgcca gacggcggac gacgccgtca cgtagagcgt gcggccgtcc
     6061 tcgccgaagg ccacgttcga gacgggcacg gggaatgcga tcgtgccgag gtgggagccg
     6121 tccttcgtga agacgtggac gccgcccggg cccgccgcga agaggttgcc ggctgcgtcg
     6181 accttcaggc cgtcgggatt ccccgtcccg gggcgcgtcc aggacgtcgc gtcgaagaaa
     6241 acgcggccgg ccgcgagcgt cccgtcgctg cggaccggga aggccatcca gacggcgcgc
     6301 cgcgcgctcg cgttcgacac gtagagcgtc ctgccgtccg gcgagaacgc gaggccgttc
     6361 ggcgcgtcga gttcctgcgt gagaagcgtg aggcggccgt cgggcgcgag gcggtacacg
     6421 ccgctgaacg gcagctcccg gcccggatcg tcgaagcccc ccgggaggcc gaatggcgga
     6481 tcggtgaagt agaggtctcc gttcgccgag aaggcgaggt cgttcggcga gttcaggcgc
     6541 gaggcgtgga atcgcccggc gaggacgctc ctcttgccgt cgtgctcgat tcgcacgatg
     6601 gcgcggtccc cgtgcgcgca gaggacgagc cggccctccg cgtcgaaggc gaggccattg
     6661 gagccgggct ccttgcccgc gaacggctcc ctgcccgtgt acccgctcgg cgacaggacg
     6721 cgcgtggtgc cggcgccggg gcgccagagg tggatcgcgt tggcgggaat gtccgagaag
     6781 aggaggccct tcgcgcgcgc gtcccagaga gggccctcga gccacgtgaa gccatcggcg
     6841 actttctcga cggcggcgcc gggcggaacg agcgcgtcga ggcgcgcgtc cttccggacg
     6901 acctgcggag cgtccgttcc ggcggcgagg agccgcgcga cgagaagggc gacgaatccg
     6961 ccgaggaggg ggagcgcggc gggaagggcc gtcagccagc ggcggagcga accgtcccgg
     7021 ctcactcggg cctcgctccc ggcacgttca gccggatccg gtagagcgac gagcgtgcgc
     7081 agaggtagag cgtccggccg tcggggtcgc cccacgccat gttgtggacg tgcttcggcc
     7141 ccgtgatcgt cccgaggtgc cggccgtcgg gcgacaggat ccagagtccg ccgggcccgg
     7201 agacgtagag gttgccgcgg gcgtccacct tgatcccgtc gatcgcgtcc tcgcccttcg
     7261 ccgaggtcat gtcgaagaag acggtcccgg ctccgagggt gccgtcgtcc cggacggggt
     7321 agcgcatcac gaccttcctc ttgtcgtccc agtttccgac gtagaggacg cgctcgtccg
     7381 gcgagaacgc gatgccgttc gggccggaga gctccttcgt gagcagcgtc agcctgccgc
     7441 cgaggattcc gaagatgccc gagaagggaa gctccttgcg cgggtcctcg aagaaccgcg
     7501 ggaggccgaa gggcgggtcg gtgaagtaga cgtcgccgtt cgaacggacg acgaggtcgt
     7561 tcgggctgtt gaggcgcttg ccctcgtagc ggtcggcgag gacggtcagc gcgccgtccc
     7621 tttcgagccg gacgacggca tggttgccgt gctggtcgaa cgtgagccgc ccttcccggt
     7681 cgagcgtgag gccgttcgaa ccgggctggc cgtattccgc gacgtcggcg cccgcgtacc
     7741 cgctcttctc gcggaagacc tcgagacggc ctgcggcgcc ggcgtcgggg gtgtagcggt
     7801 agatgcgatt gcggttggga tccgagaaaa gcagggaggt cgagcgcgcg tcccagaccg
     7861 ggccctccgt gaagagaaag ttgtcggcga cgcggaagat cttgggattg agcggaacga
     7921 tcgcgtcgag cgaggggtcg ttccgcagga cctcgacgtt cacctcctcc gggatcaccg
     7981 cgaagggttc cttcgagcgc tcggcgtaga agaggaggcg cgcgaagcgc atccagacgt
     8041 agttcgtcgg cgggttcgag agcgggccgt tcgcgccgaa gacggcgatc gagacggtgc
     8101 ggccgggctt cgcgttccgg agggcgacga cgcggttgcc tgcgttccac ccggcgacga
     8161 ccgatccgcc cgcctggccg gcgcggcgcg gcagctcgcc gtcgacccag acctcggcgt
     8221 agtcgtcgat cgacgtctcg aagacgaccg tcgagcccgc gaccgcgaag ccgccgaccc
     8281 ggtcgggcag cgtgaagcgc acgcggtacc acgcgaagga gagccggccc ccgccgcgcc
     8341 tgagcccgag ggcggcgggc gcgacggttt cccaggccga atcgtcgaag tcggcggctc
     8401 ccgcatgggg agcgatgtcc caggtgcggg aaggctccgg gccgggctgc ccgtccggcc
     8461 ccgcgagccg gaaccctgtc tcgacgggtc tcgcgtccgc gacccgccag acggcgttca
     8521 ccgccgccgc gccctccgcg ctcgcgaggt cgagcgtcgc cgcgggcgtt ccttcgggcg
     8581 ggtccccggg gccggcggcc gcga
//
LOCUS       DAOMFY010000156         8576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2532_length_8576_cov_6.163831, whole genome shotgun sequence.
ACCESSION   DAOMFY010000156 DAOMFY010000000
VERSION     DAOMFY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2532_length_8576_cov_6.163831"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PLB01_12530"
     CDS             complement(<1..1533)
                     /locus_tag="PLB01_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQQ78172.1"
                     /translation="MTRARPFRRVLVANRGEIAVRVIRGVRAAGLESVAVYSEADRGA
                     RHAALADDAVEIGPAPATESYLSIPRLLAAAKEKGCDAVHPGYGFLSENADFARAVES
                     AGLVWIGPPPEAMEAMGSKISSRERMKAAGVPVVPGALIAGGDEEKKKISLKGEEGSG
                     SESASRVAPRQPAASGTFREVLSLGLPIVVKASAGGGGKGMRIVRDPSELSEAISSCR
                     REAAAAFGDGTVYVEKYLEKPRHVEVQVFGDSHGNVVAIGERECSLQRRHQKVVEECP
                     SPAVTPELRARLAKAAVDAARAVGYENAGTVEFLLSPTGEFYFLEMNTRLQVEHPVTE
                     EAFGVDLVLLQLAVAAGETLPPLPAATARHAMEARVYAEDPEGGFLPQTGRVLLYREP
                     SGPGVRVESGLAEGQDVGVHYDPLLAKIVASGATRDEARRRLSAALGETVILGVVTNV
                     AWLRRLLGTREVAAGAIHTGLLETLAVPAPPPPPEEAFAAAAAALSGMSAPGGASAPA
                     FPD"
     gene            complement(1530..2327)
                     /locus_tag="PLB01_12535"
     CDS             complement(1530..2327)
                     /locus_tag="PLB01_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQQ78173.1"
                     /translation="MSAPKVLLEKSADPRVARLVLNRPEVRNAWDGETVTLLQRALRD
                     LASDDSVRVVELSGAGTAFCAGADLDWMKRVANFTMEENRRDSAELAQLFKALDELDK
                     PVVARVQGAALGGGTGLVAACDVVVAAEGAVFGTTEVRLGIIPAVISPYLVRKIGESH
                     ARSWFLTGERVGAEEARRVGLVHHVVGEKDLAGAAQAVIEAILLGGPAAVVEAKRLVR
                     TVSTLPAREASAWAIQRMAERRGSQEGMEGLKSFLEKRPASWTTKGS"
     gene            complement(2324..3166)
                     /locus_tag="PLB01_12540"
     CDS             complement(2324..3166)
                     /locus_tag="PLB01_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HQQ78174.1"
                     /translation="MKGRFALAAAVFVLARPAHGETRLTCGTVDALEVTSLTRTKATA
                     FLRTTLPVSPSGSKVFFEGKIELANTVLPVGQPVTALVQHGESLHEVVFLTELNLAGV
                     PSELLGRLQAAALDFTLEGNLRTSSSAPPMPVCAAGVLRVGTNQIRASGPVGRDYARF
                     GGARLAGLSLTETQGEAAVVIYNPFSFPLDVRDLVYEVRAGDRKVASGERRGVRLHAG
                     RENSIDLPVAAGNADLAAALAGAVASGGRVEGRLVAKISVKVGKDQAMTVPLDLPGTI
                     QVGK"
     gene            complement(3169..4782)
                     /locus_tag="PLB01_12545"
     CDS             complement(3169..4782)
                     /locus_tag="PLB01_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQQ78175.1"
                     /translation="MSETLASKLSPSSGEFQENAAHHRALAERLRNELAKAREGGGEK
                     ERQRHVSRGKLPVRERIERLLDPDTAFLEVAPLAGHGLYDGAAPGAGLVTGVGKVEGR
                     AVMIVANDATVKGGTYYPVTVKKHLRAQEIALENRLPTVYLVDSGGAFLPLQAEVFPD
                     RDHFGRIFYNQARMSAAGLPQVAAVLGSCTAGGAYVPAMSDETVIVKNQGTIFLAGPP
                     LVKAATGEDVTAEELGGGDVHTRLSGVADHLADDDADALEKVRGIVAHLDVPPPPPAG
                     RIAPEEPLYPAEELYGVVPKEVRRPYDVREVLARILDGSRFHEFKPRYGATLVAGFGH
                     VAGYPVAILANNGVLFSESALKATHLIEMACQRGVPLVFLQNITGFMVGRAYEHGGIA
                     KDGAKMVHAVANASVPKITLLIGGSFGAGNYGMCGRAYQPRFLFTWPNARISVMGGEQ
                     AADVLATVKDEQRTREGAPRMTEAERAEFRRPTLEKYETEGNPYYATARLWDDGIVDP
                     LDTRRTLALALATASRAPGGETRFGVFRM"
     gene            complement(4790..5200)
                     /locus_tag="PLB01_12550"
     CDS             complement(4790..5200)
                     /locus_tag="PLB01_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQQ78176.1"
                     /translation="MSDRPIRVLVAKPGLDGHDRGAKVVARALRDAGMEVIYSGLRQT
                     PQQIAAAAVQEDVDVVGLSILSGAHNVLVPEVLLALKERGGEGIAVVVGGIIPDKDIA
                     PLKAKGVRDVFLPGTSTASTVDAIRRLAGGRAGS"
     gene            5253..6506
                     /locus_tag="PLB01_12555"
     CDS             5253..6506
                     /locus_tag="PLB01_12555"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78177.1"
                     /translation="MPRLGDLLFESRLITEEQRHRAVLRQSRLRGRFGTALLEGDASE
                     DVIAHGLAVQNRVPPATPELLQSIRSDVLRLVPARVAQRLHAVPFRKEGRVLSVAMRD
                     PGDLPALDELAALTGFRIAPHAALEVRIYALLYRFYAADVEPRYLTAAAFLREVPAEP
                     KDDTDEPTQPKVGRARSKPELRVLPPPPPPDTLTPYPQRIRRMDEGDPWQAEGAKLAA
                     QVQAVRRRAEPEIFEDPLEVLDPQPESMSFPERPEPSPEAAAEEKLLARLAAARHRDD
                     VAEAVLDDALRHVKRAALFIAQPDAVVGWAARPAPNDGFGRFRLPDTVPSVFATLRNT
                     EGFYVGPQPDLPGTASTLEALASNGLGLLAVVPVNLKGKTVVYLYGEAAEGARPPSIP
                     ALKRLAAMAATGLEIVLLKTRLTRL"
     gene            6567..6905
                     /locus_tag="PLB01_12560"
     CDS             6567..6905
                     /locus_tag="PLB01_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQQ78178.1"
                     /translation="MIKNDIIIGVAAAADVPRQKATQAVETVIDVMRQALARGERIEL
                     RGFGVFLVKPRKRGIGRNPKRPTEEVQIPPGFTIRFKPGKELRDMTGTLEPGSKEPAH
                     DAPAAPATRT"
     gene            6907..7815
                     /locus_tag="PLB01_12565"
     CDS             6907..7815
                     /locus_tag="PLB01_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQQ78179.1"
                     /translation="MADVSAWAPPRPARRHERWWLHILLFVLTFLTTTYVGANFAVNF
                     RPSLLTPAGDTAATLAALFRGAITFSVPLLLILLAHELGHYLMCRRYGIDASPPYFIP
                     FPSLVGTMGAFIRIRQQIPDKRKLFDIGVAGPIAGFVVTLPLLAWGIAHTRPNPASVL
                     SEGTILFRYPLLVTLFQKLVLGTTFTSVDAVEHPVFIAAWFGLFVTALNLIPLGQLDG
                     GHALYAVFGRHQRTIAIPLLIVLAFMGLQWPGWWIWVAFTLFTGLRHPPVFDEDEPLD
                     FRRKLVAVAVLLIFVVCFAPIPLELI"
     gene            complement(7823..8236)
                     /locus_tag="PLB01_12570"
     CDS             complement(7823..8236)
                     /locus_tag="PLB01_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HQQ78180.1"
                     /translation="MIVADTGAVYALIDSRERNHSVLRKLFEKEPEAWLLPWAILPEV
                     DYLVGKYLGKKVQEEFLEDLADGSFAVEWGTESDLVEARRICRKHRDLDLGLVDACVM
                     AVAERLRATAIATLDVRDFGAVSLKGAPSLYPRDL"
     gene            complement(8236..8466)
                     /locus_tag="PLB01_12575"
     CDS             complement(8236..8466)
                     /locus_tag="PLB01_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HQQ78181.1"
                     /translation="MPLKTTVYLDEADYRRLKAIARADGRAPAELVREAVAEYARRHA
                     KRRLPKSLGAGTGDRDDAGERAEDLLKGFGRS"
BASE COUNT         1221 a         3180 c         2902 g         1273 t
ORIGIN      
        1 atcggggaac gcaggcgccg aggcgccgcc gggcgccgac atccccgaga gcgcggccgc
       61 ggccgccgcg aacgcctcct cgggtggagg cgggggcgcg ggtacggcga gagtctcgag
      121 gagacccgtg tggatcgcgc ccgcggccac ttcgcgcgtc ccgagcaggc ggcgcagcca
      181 ggcgacgttc gtgacgacgc cgaggatcac cgtctcgccg agcgcggcgg agagccggcg
      241 gcgcgcttcg tcgcgcgtcg cgcccgaggc gacgatcttc gcgaggagcg ggtcgtaatg
      301 gacgccgacg tcctggccct cggcgagccc ggactcgacg cgcactccgg ggccggaagg
      361 ctcccggtag aggaggacgc gcccggtctg cggcaggaac ccgccctcgg gatcctccgc
      421 gtagacgcgc gcctccatcg cgtgccgcgc cgtagcggcc ggcaggggcg gaagcgtctc
      481 ccccgccgcc acggcgagct ggaggaggac gaggtccacc ccgaacgctt cctcggtgac
      541 ggggtgctcg acctggaggc gcgtgttcat ctcgaggaag tagaactcgc ccgtcggcga
      601 gaggaggaac tcgaccgtgc cggcgttctc gtagccgacc gctctcgcgg cgtcgaccgc
      661 ggccttcgcg agccgcgcgc gcagctccgg cgtcacggcc ggcgacgggc actcctcgac
      721 gaccttctgg tgccggcgct gcagcgagca ctcgcgctcg ccgatcgcga cgacgtttcc
      781 gtgggaatct ccgaacacct gcacctcgac gtgcctcggc ttctcgaggt acttctccac
      841 gtacaccgtc ccgtcgccga acgcggccgc cgcttcgcgg cggcaggaag aaatggcttc
      901 ggacagctcg gaaggatcgc ggacgatgcg cattcccttc ccgcctccac ctgctgaggc
      961 tttgacgacg ataggaagac cgagagaaag aacttctctg aaggtgccag aggctgcggg
     1021 ctgccgcggc gccactcgac tcgccgattc gctcccactc ccctcttcac ctttcaaaga
     1081 aatcttcttc ttctcttcgt ctcctcccgc gatgagggcg cccggcacga ccggcacccc
     1141 cgccgccttc atcctctctc tcgaagaaat cttgctcccc atcgcctcca tcgcctcggg
     1201 cggggggccg atccacacga ggccggccga ctcgacggcg cgcgcgaaat ccgcgttctc
     1261 ggagaggaag ccgtagccgg gatgcacggc gtcgcagccc ttctccttcg cggcggcgag
     1321 gagcctcggg atcgacaggt agctctccgt cgcgggcgcc ggcccgatct caaccgcgtc
     1381 gtccgcgagc gcggcgtggc gggcgccgcg gtccgcctcg gagtacaccg cgacggattc
     1441 gaggcccgcc gcgcggacgc cgcggatcac gcgcaccgcg atctcgccgc ggttcgcgac
     1501 gagcacccgc cggaagggcc gcgcccgcgt cacgacccct tcgtcgtcca gctcgcgggg
     1561 cgcttctcga ggaaggactt caggccctcc atcccctcct gcgagccgcg cctctcggcc
     1621 atccgctgga tggcccaggc cgacgcttcc cgcgccggca gcgtcgagac ggtgcggacg
     1681 aggcgcttgg cctcgacgac ggccgccggc ccgcccagga ggatcgcttc gatcaccgcc
     1741 tgcgcggcgc cggccaggtc cttctccccg acgacgtggt ggacgagccc gacgcgccgc
     1801 gcctcttcgg cgcccacgcg ctctcccgtc aggaaccacg agcgcgcgtg ggactcgccg
     1861 atcttccgga cgagataagg cgagatcacg gccgggatga tcccgaggcg cacctcggtc
     1921 gtcccgaaga cggccccctc ggccgcgacg acgacgtcgc acgcggcgac gaggccggtg
     1981 ccgccgccca gcgcggcgcc ctgcacgcgc gcgacgaccg gcttgtcgag ctcgtcgagc
     2041 gccttgaaga gctgcgcgag ctcggccgag tcgcgccggt tctcctccat cgtgaagttc
     2101 gcgacgcgct tcatccagtc gaggtcggcg cccgcgcaga acgccgttcc cgcgcccgag
     2161 agctcgacga cgcgcacgga gtcgtcggag gcgaggtcgc ggagcgcgcg ctgcaggagc
     2221 gtcacggtct cgccgtccca ggcgttgcgc acctcgggcc ggttcaggac gagacgcgcc
     2281 acgcgcgggt cggcggactt ctcgaggagg actttcgggg cgctcacttg cccacctgga
     2341 tcgtgccggg gagatcgagc ggaaccgtca tggcctggtc ctttccgact ttcacggaga
     2401 tcttcgcgac gagccgtccc tccacgcgcc cgcccgacgc cacggcgccc gcgagcgcgg
     2461 ccgcgaggtc cgcgttgccg gcggcaaccg gcaagtcgat cgagttctcg cggccggcgt
     2521 gcagccgcac gccgcgccgc tcgccggacg cgaccttccg gtcgccggcg cggacctcgt
     2581 aaacgaggtc ccggacgtcc agcgggaacg agaacgggtt gtagatcacg acggccgcct
     2641 ctccctgcgt ctcggtgagc gagagcccgg ccaggcgtgc gccgccgaag cgggcgtagt
     2701 cccgcccgac gggaccgctc gcgcggatct ggttcgtccc gaccctgagg actccggccg
     2761 cgcacacggg catcggaggg gcggaagagg aagttcggag atttccttcg agtgtgaaat
     2821 caagggcggc ggcctgcagt cttccaagaa gctcggatgg aacgccggcg agattcagct
     2881 cggtgaggaa caccacctcg tgaaggctct cgccgtgctg gacgagcgcc gtcaccggct
     2941 gacccaccgg cagaaccgtg ttcgcgagct cgatcttccc ttcgaagaag accttcgagc
     3001 cggaagggga aacgggaagc gtggttcgaa ggaaggccgt ggccttggtc cgcgtcagcg
     3061 aggtcacctc gagggcgtcc acggttccgc acgtcaggcg cgtctcgccg tgagcgggac
     3121 gggcgagaac gaatacggcc gcggcgaggg cgaagcgtcc cttcatctct acatccggaa
     3181 gacgccgaaa cgcgtctccc ctcccggcgc gcgcgaggcc gtcgcgagcg cgagcgcgag
     3241 cgtgcggcgc gtgtcgagcg ggtcgacgat cccgtcgtcc cacaggcgcg ccgtcgcgta
     3301 gtacggattt ccctcggtct cgtacttctc cagcgtcggg cggcggaact ccgcgcgctc
     3361 ggcctcggtc atccgcggcg cgccttcgcg cgtgcgctgc tcgtccttca cggtcgcgag
     3421 gacgtcggcc gcctgctcgc cgcccatcac ggagatgcgc gcgttcggcc acgtgaacag
     3481 gaaacggggc tggtacgccc ggccgcacat gccgtagttc ccggcgccga acgagccccc
     3541 gatcaggagc gtgatcttcg gaacggacgc attcgcgacg gcgtggacca tcttggcgcc
     3601 gtccttggcg atcccgccgt gctcgtaggc ccgcccgacc atgaagcccg tgatgttctg
     3661 gaggaacacg agcggcaccc cgcgctggca ggccatttcg atcaggtgcg tcgccttcag
     3721 ggcggactcc gagaagagca cgccgttgtt cgcgagaatc gcgaccgggt agcccgcgac
     3781 gtgcccgaag ccggccacga gcgtcgcgcc gtagcgcggc ttgaactcgt ggaagcgcga
     3841 gccgtcgagg atgcgcgcga ggacctcgcg cacgtcgtac gggcggcgga cttccttcgg
     3901 gacgacgccg tagagctcct ccgccgggta gagcggctcc tcgggcgcga tgcggcccgc
     3961 gggcggcggc ggcggcacgt cgaggtgcgc gacgattccg cgcacctttt cgagcgcgtc
     4021 cgcgtcgtcg tccgcgaggt ggtccgccac gcccgagagg cgcgtgtgga cgtcgccgcc
     4081 gccgagctcc tcggcggtga cgtcctcgcc cgtggcggcc tttacgagcg gcggccccgc
     4141 gaggaagatc gtgccctggt tcttgacgat gacggtctcg tccgacatcg ccggaacgta
     4201 cgcgccgccg gcggtgcacg agccgaggac cgcggcgacc tgcgggaggc ccgcggcgct
     4261 catgcgcgcc tggttgtaga agatgcggcc gaagtggtcg cggtccggga agacctcggc
     4321 ctgcagcggc aggaacgcgc cgccggagtc cacgagatag accgtcggga gccggttctc
     4381 gagcgcgatc tcctgggcgc gcaggtgctt cttcaccgtg acggggtaat acgtcccgcc
     4441 tttcacggtc gcgtcgttcg cgacgatcat gacggcccgc ccctcgacct tgccgacgcc
     4501 ggtcacgagg ccggcgcccg gtgcggcacc gtcgtagaga ccgtggcccg cgagcggtgc
     4561 gacctcgagg aacgcggtgt cggggtcgag aaggcgctcg atcctctcgc gcacggggag
     4621 ctttccgcgc gacacgtggc gctgccgctc cttctcgccg ccgccttccc gggccttcgc
     4681 gagctcgttc ctcaggcgtt ccgcgagcgc ccggtggtgg gcggcgttct cctggaactc
     4741 gccggaagac ggcgagagct tcgacgcgag ggtttcgctc acggacggat caggagccgg
     4801 cccgtccgcc cgcgaggcgg cggatcgcgt cgaccgttga ggccgtgctc gtgcccggca
     4861 ggaagacgtc gcggaccccc ttcgccttca ggggcgcgat gtccttgtcc gggatgatcc
     4921 cgccgacgac gacggcgatt ccctctccgc cgcgctcctt gagagcgaga aggacttcgg
     4981 gcacgaggac gttgtgggcg ccggagagga tcgagaggcc gaccacgtcg acgtcctcct
     5041 ggacggcggc ggccgcgatc tgctgcgggg tctggcgcag gcccgagtag atgacttcca
     5101 tgccggcgtc acgcagcgcg cgggcgacga ccttggcgcc ccggtcgtga ccgtcgaggc
     5161 ccggcttggc gacgagaacg cggatcggac ggtcgctcat gcccgcgatt ctacccgtgc
     5221 gccacgtgta aactcgccgg gaaagtctcc gcatgccgcg actgggtgat ctcctcttcg
     5281 aaagcaggct catcaccgag gagcaacggc accgcgccgt cctccggcag agccgtctgc
     5341 gcggccggtt cggaaccgct ctgctcgaag gggacgcctc ggaggacgta atcgcccacg
     5401 gcctggccgt gcaaaaccgc gtgccgccgg ccacgcccga gctcctccag tcgatccgct
     5461 ccgacgtcct ccggctcgtc cccgcccgcg tggcccagcg gctgcacgcc gtgccgttcc
     5521 gaaaggaggg ccgcgtcctc tcggtcgcga tgcgcgaccc cggagacctc cccgccctcg
     5581 acgagctcgc ggccctcacg ggattccgca tcgccccgca cgcggcgctc gaggtccgga
     5641 tctacgcgct cctctaccgc ttctacgcgg ccgacgtcga gccgcgctac ctcacggccg
     5701 cggcgttcct gcgcgaggtc ccggccgagc cgaaggacga caccgacgag ccgacgcagc
     5761 cgaaggtcgg gcgcgcgcga agcaagcccg aactccgcgt cctcccgccc cctccgccgc
     5821 ccgacacgct cacgccgtat ccgcagcgca tccggcgcat ggacgaaggc gacccgtggc
     5881 aggccgaggg cgcgaagctc gccgcgcagg tccaggcggt ccgcaggcgt gccgagcccg
     5941 agatcttcga ggatccgctc gaggtcctcg acccgcagcc cgagtcgatg agctttcccg
     6001 agcggcccga acccagcccc gaggcggccg cagaggagaa gctgctcgcg cgcctcgcgg
     6061 ccgcacgcca ccgcgacgac gtcgccgaag cggtcctcga cgacgcgctc cgccacgtga
     6121 agcgagccgc tctcttcatc gcccagcccg atgcggtcgt gggctgggcc gcccggcccg
     6181 cgccgaacga cggtttcggc cgttttcgcc tgcccgacac cgtgccgtcc gtctttgcga
     6241 cgctgcgcaa tacggagggg ttctacgtcg gcccgcagcc cgacctgccg ggaacggcct
     6301 cgacgctcga ggcgctcgcc tccaacggtc tcggcctcct cgccgtcgtg cccgtgaacc
     6361 tgaagggaaa gaccgtcgtc tacctctacg gcgaggccgc cgagggggcg aggccgccct
     6421 cgatccccgc gctcaagcgc ctggcggcca tggcggccac ggggctcgag atcgtcctcc
     6481 tcaagacccg gctcacccgc ctttgacggg tgctaacggg ctcgatttga acgacatggg
     6541 gccgaccggg taaactccgc gaacacgtga tcaagaacga catcatcatc ggcgttgccg
     6601 cagcggccga cgtgccgcgg cagaaagcca cgcaggccgt cgagacggtc atcgacgtca
     6661 tgcgccaggc cctcgcgcgc ggcgagcgca tcgagctgcg cggcttcggc gtgttcctcg
     6721 tcaagccgcg caagcgcggc atcgggcgca acccgaagcg gccgaccgag gaagtccaga
     6781 tcccgcccgg gttcacgatc cgcttcaagc ccggcaagga gctccgcgac atgaccggca
     6841 cgctcgagcc gggttcgaag gagcccgccc acgacgcccc cgccgccccc gccacccgca
     6901 cctgacgtgg cggacgtctc ggcctgggcg ccgccgcggc cggcgcgccg ccacgagcgc
     6961 tggtggctcc acatcctcct gttcgtcctg acgttcctga cgacgacgta cgtcggcgcg
     7021 aacttcgccg tcaacttccg cccgagcctc ctgacgccgg ccggcgacac ggcggcgacg
     7081 ctggccgcgc tgtttcgcgg cgccatcacc ttctcggtgc cgctgctcct catcctcctc
     7141 gcccacgagc tgggccacta cctgatgtgc cgccggtacg ggatcgacgc ctcgccgccg
     7201 tacttcatcc cgttcccgtc gctcgtcggg accatgggcg ccttcatccg catccgccag
     7261 cagattcccg acaagaggaa gctgttcgac atcggagtcg cggggccgat cgccggcttc
     7321 gtcgtgaccc tgcccctcct ggcctggggc atcgcgcaca cgcgcccgaa cccggcctcc
     7381 gtcctctccg agggcacgat cctcttccgc tacccgcttc tcgtgacgct cttccagaag
     7441 ctcgtcctcg ggacgacgtt cacgagcgtc gacgcggtcg agcacccggt cttcatcgcg
     7501 gcctggttcg gcctcttcgt cacggcgctg aacctcatcc cgctcggtca gctcgacggc
     7561 gggcacgccc tctacgcggt cttcggccgg caccagcgga cgatcgcgat cccgctcctc
     7621 atcgtcctcg ccttcatggg cctgcagtgg cccggctggt ggatctgggt cgcgttcacg
     7681 ctgttcacgg ggctgcggca cccgcccgtc ttcgacgagg acgagccgct cgacttccgc
     7741 cgcaagctcg tggcggtcgc cgtgctcctc atcttcgtcg tctgcttcgc gccgattcct
     7801 ctcgagctga tctgacgggt cttcagaggt cgcggggata gaggctcggg gcgcccttga
     7861 gtgatacggc gccgaaatcc ctcacatcga gagtcgcgat cgccgtagct ctcagtcgct
     7921 cggcgacggc catcacacac gcgtcgacaa ggcccaggtc gagatcccgg tgcttccgac
     7981 aaatccgccg cgcctccacg agatcgctct ccgtgcccca ttcaacggcg aacgacccat
     8041 cggcgaggtc ttcgaggaac tcctcctgga ccttttttcc gagatatttt ccgacgagat
     8101 agtcgacctc gggaaggatc gcccacggaa ggagccacgc ctcgggctcc ttctcgaaga
     8161 gcttccgaag gacggagtgg ttccgctcgc gggagtcgat cagcgcgtag acggctcccg
     8221 tgtccgcgac gatcatcagg accggccgaa ccccttcaga aggtcctcgg cccgctcacc
     8281 ggcgtcgtct ctgtcccccg tacccgctcc gaggctcttc ggcagccggc gctttgcgtg
     8341 ccggcgcgcg tattcggcga cggcctcgcg aacgagctcg gcgggcgccc gcccgtccgc
     8401 ccgcgcgatc gccttcaggc ggcggtagtc ggcctcgtcg aggtagacgg tcgtcttgag
     8461 cggcatatgg caaccatata tggcgtcagc tcgaacgtca actcctcttc ttccgccgca
     8521 gcgcgccgag tgcctcgccc agctcgggtg cccggagcag cgccgcgaag gcgagg
//
LOCUS       DAOMFY010000157         8570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2537_length_8570_cov_8.169231, whole genome shotgun sequence.
ACCESSION   DAOMFY010000157 DAOMFY010000000
VERSION     DAOMFY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_8570_cov_8.169231"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLB01_12580"
     CDS             complement(<1..891)
                     /locus_tag="PLB01_12580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HQQ78182.1"
                     /translation="MSAQPSGPDGVAAAVLPSAAADVEGAAPDPGHAAHVEASRRSIL
                     AAIGAPEDVTFVRGVGNVGDQLIAAGSRALLAGVLTREIGLADVPGARGELAVLCGSG
                     AWSGPYHELMPPILPVLERRFERVVVLPSSFDVSVPEVRHVLETTRATVFARERDSFD
                     GIRGLCDARLAHDGAFYFDFAPWRRSGRGALDAFRTDREAARGPLPPGNVDVSVACAT
                     LDEWLHTLARHALIRTDRAHVLIAAALLGKTVEWAASSTRKLPAIAAFALDGRFPVRR
                     LDHPASHGRAAAVPSTASPRG"
     gene            complement(965..1429)
                     /locus_tag="PLB01_12585"
     CDS             complement(965..1429)
                     /locus_tag="PLB01_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQQ78183.1"
                     /translation="MKVRDIMSTKVQTIDMNEKAESAWNLMKWKRIHHVVVTDGPGQV
                     VGVVSARDLGGQDREEARKIRPVAAMMTAYAVKAVPEMPVRQAANVMRGWNIGCLPVI
                     GDGKLVGIVTVSDLLRIVAEGADKPAAAPKKAAAKKAPAKKTAKKKAGKRTR"
     gene            complement(1492..2823)
                     /locus_tag="PLB01_12590"
     CDS             complement(1492..2823)
                     /locus_tag="PLB01_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ78184.1"
                     /translation="MNPRSLLSRFVRVEPGEGRGLVWAAASFFFLLGGYYIIRPLREE
                     MGASSGGGFALNWLFLGTLAGTFLVTPLFGALVSKFPRRVFVPAVYHALAASFVAFYV
                     ALSVLAGPQRLATARFFFVFVSTFNLLIVSVFWGFLADLFRSEQAKRLFGAIGLGGTL
                     GGISGGVITASLAKALGPVSLLLVAAGMFEAAVFCMGRLARTYRVDEAGPSAAEAGDE
                     PSGGLPPGTGALSGLSLFAKSRYLLGIAAFLLLFTVGSTFLYLAQARIVRATFADAGS
                     RTAFFAKADLAVNVLTAITQLFFTGRLLPALGLGPALAFLPVLSAAGFFALGGSPTAG
                     TLFVVQVVRRAAEFALVKPAREVLFTVVSREEKYSAKSFIDTFVYRSGDALGALADRL
                     VAFVGLGPVGFAWAFLPVAGLWTWNALALGSRQRALAEDREGPARALPAAP"
     gene            complement(2829..3917)
                     /locus_tag="PLB01_12595"
     CDS             complement(2829..3917)
                     /locus_tag="PLB01_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HQQ78185.1"
                     /translation="MQTAAAAAAGSALGTNRLLSGAEPKTLLILGGTRFLGPEVVEAA
                     KKSGWKITLFNRGKSHPDWFPELEHLQGDRNGDLKSLEGRAWDAVVDTSGYFPRQVAA
                     SAGLLSKSKQYVFISSISVFSDLSKPGVDENGPVGELKDPNADKITEGNYGPLKAACE
                     RAAEAAMPGRVTNVRPGLIVGPNDLTDRFTYWPVRVARGGEVLAPNKPSDFVQFIDVR
                     DLGTWIVKCLDAKTFGVFNATGNPVPVGTLLDTCKAVSGSNATFTWADAAFLEAQKVA
                     AWSDLPCWLPPTGEEAHGNEVSNAKAVGKGLTFRPLKNTCRDTLAWWKTLPAERQVKL
                     KSGLTAEREAAVLAAWHEAQAKKAPKAG"
     gene            complement(3950..4825)
                     /gene="rfbA"
                     /locus_tag="PLB01_12600"
     CDS             complement(3950..4825)
                     /gene="rfbA"
                     /locus_tag="PLB01_12600"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HQQ78186.1"
                     /translation="MKGIILAGGSGTRLYPLTIGLSKQLCPVYDKPMVYYPLSTLMLA
                     GLRDVLVITTPEDQASFMRLLGDGSQFGIRISYAAQPKPEGLAQAFLIGKDFVGQDGV
                     ALALGDNLFYGHGLPEMLQRAAGRASGATVFAYRVLDPQRYGVVEFDARGRAVGLEEK
                     PPAPRSNYAVTGLYFYDNRVLDVAANLKPSARGELEITDVNRAYLATGDLHVEILGRG
                     MAWLDTGTHEALLQASNFIQAIEARQGLKVACPEEIAWNAGWISSEDVARLAIRMNKN
                     EYGAYLLRMLEEARL"
     gene            complement(4822..5925)
                     /gene="rfbB"
                     /locus_tag="PLB01_12605"
     CDS             complement(4822..5925)
                     /gene="rfbB"
                     /locus_tag="PLB01_12605"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQQ78187.1"
                     /translation="MDSILVTGGAGFIGSNFVRLALKETPGRVAVVDKLTYSGHRASL
                     ADCEKDPRFSFVEADIADAAAMKAAFAAHRPRAVLNFAAETHVDRSIDGPGAFIHTNV
                     TGTYVLLEASRAHAASLPPGEREAFRFLHVSTDEVYGTLGPTGLFSEDTPYAPNSPYA
                     ASKAAADHLVRAWHETYGLPTLLTNCSNNYGPYQFPEKLIPLMILNALEGKPLPIYGD
                     GGNVRDWLHVDDHCRGILLVLEKAAPGSKYNIGGGNERTNLQIVDALCEAVERVRPAE
                     ENPKISLKGKKGYSQLKTFVPDRPGHDRRYAIDASKIRRELGWVPSYSFEEGLLRTVE
                     WYVSHPEWCATVQSGNYGRERLGLAAPADGVRA"
     gene            5996..7366
                     /locus_tag="PLB01_12610"
     CDS             5996..7366
                     /locus_tag="PLB01_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HQQ78188.1"
                     /translation="MRETARFLLDEARLPKAWYNVNADMPVPPTPVLHPGTLEPVTPD
                     FLSVLFPMNLVMQEVSGERWIEIPEPVREIYRLWRPTPMHRALRLEKALDTPAHIYFK
                     NESVSPVGSHKTNTAVAQAFFNKEAGTKALTTETGAGQWGSALSLACNFFDLDLEIFM
                     VKVSYHQKPYRRHIMENFGAQVFASPSDRTSFGRRVLAESPDSPGSLGMAISEAVEVA
                     ATSGGAKKYSLGSVLGHVLMHQTVIGIEALEQMEMAGEYPDVVIGCAGGGSNFAGFAY
                     PFVHRNLTQGKKTRIVAVEPASCPTLTKGRYTFDFGDTAATAPVVKMHTLGHTFVPPG
                     IHAGGLRYHGMAPSVSALVDHGDIEARSARQLETFEAAVLFSKAEGIIPAPESAHAIR
                     AAIDEALRCKAAGERKVIAFNLSGHGHFDMTAYQSFHAGELKDFEYPDDAVEEAMSHL
                     PKVAGG"
     gene            7424..>8570
                     /locus_tag="PLB01_12615"
     CDS             7424..>8570
                     /locus_tag="PLB01_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78189.1"
                     /translation="MRRGAFAAAGIAGAALLAVLVPLYDAPEPRGLTVARGKVRDVAD
                     AEARRWGIEVDKAFVVTTLDNSPLLDKELRDKPELRRRAESDPVLGPRIFGFRVTYWR
                     NGVDKYPPYGFVTVSRAGDVVAAMRQARLEETGASPKADDLRAKADAFVASRAFAGAP
                     SPVFEDVRPNVQRNRADHTFRYRVPSSFPTGDVVCYLYVTFIGEKPAGWSLIEEYRDG
                     RRFAYDAGIGETFLRFAGIFSLLAVLLAVFLRKYHAGEVGVGTGAALFAAAIGLSIVH
                     NLLTAPWAASGTGLGSIDNRTTTLAVSGFRFLFYDIPLSLLVFLAWSVGESFARERWG
                     ERLASFDALLRRDVRNATVGGSLLAGLLAAPAVAAAALLVPALPFRAG"
BASE COUNT         1284 a         2912 c         3123 g         1251 t
ORIGIN      
        1 tccgcgaggg gacgccgtgc ttggaacggc agcggcgcgg ccgtgggacg cggggtggtc
       61 gaggcggcgg acgggaaagc ggccgtcgag cgcaaatgcg gcgattgcgg gcagcttgcg
      121 cgtgctcgac gcggcccatt cgaccgtctt tccgaggagc gcggccgcga tcaggacgtg
      181 cgcgcggtcc gtccggatga gggcgtggcg cgcgagcgtg tgcagccatt cgtccagcgt
      241 cgcgcaggcg acggagacgt cgacgtttcc gggagggagc ggcccgcggg cggcttcacg
      301 gtccgtgcgg aacgcgtcga gggcgccgcg gccgctccgg cgccagggcg cgaagtcgaa
      361 gtagaacgcc ccgtcgtgcg cgagccgcgc gtcgcaaagg ccgcggatcc cgtcgaagga
      421 gtcgcgctcg cgggcgaaga cggtggctcg cgtcgtctcg agaacgtgcc gcacctcggg
      481 cacggagacg tcgaaggagg acggcaggac gacgacgcgc tcgaaccgcc gctcgaggac
      541 cgggaggatc ggcggcatca gctcgtggta cggaccggac cacgcgccgc tcccgcagag
      601 aacggccagc tcaccgcggg cgcccggaac gtcggcgagg ccgatttcgc gcgtcaggac
      661 tcccgcgagc agggcgcggg acccggcggc gatgagctgg tctccgacgt tgccgacccc
      721 gcggacgaac gtgacgtcct cgggggcgcc gatcgcggcc aggatcgaac ggcggctcgc
      781 ctcgacgtgc gcggcgtggc cgggatccgg ggcggcacct tccacgtctg ccgcggccga
      841 agggaggacc gcagcagcga ctccgtccgg gccgctgggc tgtgcgctca tgaggaaggc
      901 gcgaatgata gatcagtcgc gcctcgcgcg aggaggcgcg tggccggggc gcccgccgcc
      961 gggctcagcg cgtccgcttc ccggccttct tcttcgcggt tttcttcgcg ggcgctttct
     1021 tcgcggccgc tttcttcggg gccgcggccg gcttgtcggc gccctcggcg acgatgcgaa
     1081 gcaggtccga caccgtcacg atgccgacga gcttgccgtc gccgatgacg ggcaggcagc
     1141 cgatgttcca gccgcgcatg acgttcgcgg cctggcggac cggcatttcg ggaacggcct
     1201 tgacggcgta ggcggtcatc atcgcggcga cggggcggat cttgcgcgct tcctcgcggt
     1261 cctggccgcc gaggtcgcgg gccgagacga cgccgacgac ctgcccgggc ccgtccgtga
     1321 cgacgacgtg gtggatgcgc ttccacttca tgaggttcca ggcgctctcg gccttctcgt
     1381 tcatgtcgat cgtctggacc ttcgtggaca tgatgtcgcg gactttcatt gcagaaatct
     1441 ccccatcaaa gagtcggggc gggcccgggc cgccgccatg agccacttga atcaaggggc
     1501 ggcggggagc gcgcgcgccg ggccctcccg gtcttccgcg agcgcgcgct ggcggctgcc
     1561 gagggcgagg gcattccacg tccacaggcc cgccacgggg aggaacgccc acgcgaaccc
     1621 caccggcccg agcccgacga aggccacgag ccggtccgcg agggcgccga gcgcgtcgcc
     1681 gctccggtag acgaacgtgt cgatgaagct cttggccgag tacttctctt cccgggagac
     1741 gaccgtgaag aggacttcgc gcgccggctt cacgagggcg aactccgcgg cgcgacgcac
     1801 gacctggacg acgaacagcg tcccggccgt gggagacccg ccgagcgcga agaagcccgc
     1861 ggccgacagg acggggagga acgcgagcgc gggcccgagg ccgagggccg gcaggagccg
     1921 gcccgtgaag aagagctggg tgatcgccgt caggacgttg acggcgaggt ccgccttcgc
     1981 gaagaacgcg gtccgcgacc cggcgtcggc gaacgtcgcg cgcacgatgc gcgcctgcgc
     2041 gaggtagagg aacgtcgacc cgacggtgaa gagcaggagg aacgcggcga tgccgagcag
     2101 gtagcgcgac ttcgcgaaga gggagagccc ggagagggct cccgtgccgg gcgggaggcc
     2161 gccggagggc tcgtccccgg cctcggcggc ggaagggccg gcctcgtcga cgcggtacgt
     2221 gcgcgcgagc cggcccatgc agaagacggc cgcctcgaac attccggccg cgacgaggag
     2281 aagcgacacc gggccgagcg ccttcgcgag gctggccgtg atcaccccgc cggagatgcc
     2341 gcccagcgtg ccgccgaggc cgatcgcccc gaacagccgc ttggcctgct cggaccggaa
     2401 gaggtcggcg aggaagcccc agaagacgga gacgatgagg aggttgaacg tgctcacgaa
     2461 gacgaagaag aagcgcgcgg tcgcgagccg ctgcgggccc gcgaggacgc tcagcgcgac
     2521 gtagaacgcg acgaagctcg ccgcgagcgc gtggtagacc gcgggaacga agacgcggcg
     2581 cgggaacttc gagacgagcg cgccgaagag cggcgtcacg aggaacgtgc ccgcgagcgt
     2641 gccgaggaac agccagttca gggcgaatcc gccgccggag gacgcgccca tctcctcgcg
     2701 gagcggccgg atgatgtagt acccgcccag caggaagaag aaggaggcgg ccgcccagac
     2761 gagtccgcgg ccctcgccgg gctcgacccg cacgaagcgg gacaggaggc tccggggatt
     2821 caccgttttc agccggcctt cggagccttc ttcgcctgcg cctcgtgcca cgccgcgagg
     2881 accgcggcct cgcgctcggc cgtcaacccc gacttgagct tcacctgccg ctcggccggg
     2941 agcgtcttcc accacgcgag cgtgtccctg caggtgttct tgagcggccg gaacgtcagg
     3001 cccttgccga cggccttcgc gttcgagacc tcgttcccgt gcgcctcctc gccggtcggc
     3061 ggaagccagc aggggaggtc gctccacgcg gccaccttct gcgcctcgag gaacgcggcg
     3121 tccgcccacg tgaacgtcgc attgctgccg ctcacggcct tgcaggtgtc gaggagcgtc
     3181 ccgacgggga cggggttccc cgtcgcgttg aagactccga acgtcttcgc gtcgaggcac
     3241 ttcacgatcc aggtgccgag atcgcgcacg tcgatgaact gcacgaagtc ggacggcttg
     3301 ttcggtgcga ggacctcgcc gcccctcgcg acgcggacgg gccagtacgt gaagcggtcc
     3361 gtgaggtcgt tcgggccgac gatgaggccg ggacggacgt tcgtgacgcg gcccggcatc
     3421 gcggcctccg ccgcccgctc gcaggcggcc ttgaggggcc cgtaattccc ttccgtgatc
     3481 ttgtcggcgt tcgggtcctt gagctcgccg accgggccgt tctcgtcgac gcccggcttg
     3541 ctgaggtcgg agaacaccga gatggacgag atgaagacgt actgcttcga cttcgagagg
     3601 aggccggcgg acgcggcgac ctgcctcggg aagtagccgg acgtgtccac cacggcgtcc
     3661 caggcgcggc cctcgaggga cttgaggtcg ccgttgcggt caccctgcag gtgctccagc
     3721 tccgggaacc agtccgggtg ggacttgccc ctgttgaaga gcgtgatctt ccagccgctc
     3781 ttcttcgcgg cctcgacgac ctcggggccg aggaagcgcg ttcccccgag gatcaggagc
     3841 gttttcggct ccgcacccga aagcaggcgg ttcgtcccca gggcggagcc ggctgcggcc
     3901 gcggcggcgg tctgcaagag acgacggcgg gtcgtacggg gggtcaggct cacaaacgtg
     3961 cctcctcgag catgcgaagg agatacgcac cgtattcgtt cttgttcatc cggatggcga
     4021 gccgggcgac gtcttccgag gagatccatc cggcgttcca ggcgatctcc tcgggacagg
     4081 cgaccttcag cccctgccgg gcttcgatgg cctggatgaa gttggaggcc tgaagcagcg
     4141 cctcgtgggt gcccgtgtcg agccacgcca tcccccgccc gaggatctcg acgtggaggt
     4201 cgccggtcgc gaggtacgcg cggttcacgt ccgtgatctc gagctcgccg cgggccgagg
     4261 gcttgaggtt cgcggccacg tcgagcacgc ggttgtcgta gaagtagagg cccgtgacgg
     4321 cgtagttcga ccggggcgcg ggcggcttct cctcgaggcc gaccgcgcgt ccccgggcgt
     4381 cgaactcgac gacgccgtac cgctgcgggt cgaggacgcg atacgcgaac accgtcgcgc
     4441 ccgaagcgcg gcccgcggcg cgctgcagca tctcgggcag gccgtgcccg tagaagaggt
     4501 tgtcgccgag cgcgagggcg acgccgtcct gcccgacgaa gtccttcccg atcaggaacg
     4561 cctgcgcgag gccctcgggc ttcggctgag ccgcgtagga gatgcggatc ccgaactgcg
     4621 agccgtcgcc gaggaggcgc atgaacgagg cctggtcctc cggcgtcgtg atcacgagga
     4681 cgtcgcgcag ccccgcgagc atgagcgtcg agagcgggta atagaccatc ggcttgtcgt
     4741 agacggggca gagctgcttg gagaggccga tggtgagggg atagaggcgc gtgcccgagc
     4801 cgccggcgag gatgatcccc ttcacgcgcg gactccgtcc gcgggggccg cgagcccgag
     4861 gcgctcgcgg ccgtaattcc cggactgcac ggtcgcgcac cactcggggt gactcacgta
     4921 ccactccacg gtccgaagaa gaccttcttc gaatgagtaa gaggggaccc agcccaattc
     4981 gcggcggatc ttcgacgcgt cgatggcgta gcggcggtca tggcctggcc gatccggcac
     5041 gaacgttttg agctgcgagt agcccttctt acccttcaaa gaaatcttcg ggttctcttc
     5101 ggccggccgc acgcgctcaa cggcttcgca tagcgcgtcg acgatctgca ggttcgtgcg
     5161 ctcgttgccg ccgccgatgt tgtacttgct ccccggcgcc gccttctcga gcacgagcag
     5221 gatgccgcgg cagtggtcgt cgacgtgcag ccagtcgcgc acgttgccgc cgtctccgta
     5281 gatcgggagg ggcttgccct cgagcgcgtt gaggatcatg agcgggatga gcttctcggg
     5341 gaactggtac ggcccgtagt tgttcgagca gttcgtgagg agggtcggga ggccgtacgt
     5401 ctcgtgccag gcgcgcacga ggtggtcggc ggccgccttg gacgccgcgt acggggagtt
     5461 cggggcgtac ggggtgtcct cggagaagag gcccgtcggc ccgagcgtgc cgtagacctc
     5521 gtccgtcgag acgtggagga accggaacgc ctcgcgctcg cccggcggga gggaggccgc
     5581 gtgcgcccgc gacgcctcga ggaggacgta cgtgcccgtc acgttcgtgt ggatgaaggc
     5641 gcccgggccg tcgatcgagc ggtcgacgtg cgtctcggcg gcgaagttca ggacggcgcg
     5701 ggggcggtgc gccgcgaagg ccgccttcat ggccgcggcg tccgcgatgt cggcctcgac
     5761 gaaggagaac cgggggtcct tctcgcagtc cgcgaggctc gcccggtgcc cggagtacgt
     5821 cagcttgtcg acgacggcca cccggccggg ggtctccttg agagcgaggc ggacgaagtt
     5881 cgagccgatg aaaccggctc cgccggtgac gaggattgag tccatgcagg cgggaggata
     5941 tcggagtcgg gccgctcctg cgttatcgtc ccgcctttcc ggtggaggag gatccatgcg
     6001 cgagaccgca cgtttcctgc tggacgaagc gcggctcccg aaggcctggt acaacgtgaa
     6061 cgcggacatg ccggtgccgc cgacgcccgt gctgcacccc gggacgctcg agcccgtgac
     6121 gcccgacttc ctgtccgtcc tcttcccgat gaacctcgtg atgcaggagg tctcggggga
     6181 gcgctggatc gagatcccgg agcccgtccg ggagatctac cgcctctggc gcccgacgcc
     6241 gatgcaccgg gcgctgcgcc tcgagaaggc gctcgacacg ccggcccaca tctatttcaa
     6301 gaacgagagc gtgagcccgg tgggctcgca taagacgaac acggccgtgg cgcaggcctt
     6361 cttcaacaag gaagccggga cgaaggctct gacgacggag accggcgccg ggcagtgggg
     6421 ctcggccctg tccctcgcct gcaacttctt cgacctcgac ctcgagatct tcatggtgaa
     6481 ggtctcgtac caccagaagc cgtaccggcg gcacatcatg gagaacttcg gggcgcaggt
     6541 cttcgcgagc ccgtcggacc ggacgtcgtt cgggcgccgg gtcctcgccg agtcgcccga
     6601 ctccccgggc tccctcggga tggcgatctc cgaggccgtc gaagtcgccg cgacgagcgg
     6661 cggggcgaag aagtacagcc tcggctccgt cctcgggcac gtcctcatgc accagacggt
     6721 catcgggatc gaggcgctcg agcagatgga gatggcgggg gagtacccgg acgtcgtgat
     6781 cggctgcgcg ggcggcgggt cgaacttcgc gggcttcgcg tacccgttcg tccaccggaa
     6841 cctcacgcag gggaagaaga cgcgcatcgt cgccgtcgag cccgcgtcct gcccgacgct
     6901 cacgaagggc cgctacacct tcgatttcgg cgacacggcc gcgacggctc cggtcgtcaa
     6961 gatgcacacg ctcgggcaca cgttcgtgcc gcccgggatc cacgcgggcg gtctccggta
     7021 ccacgggatg gcgccgtccg tcagcgcgct cgtcgaccac ggcgacatcg aggcgcgttc
     7081 ggcgcggcag ctcgagacgt tcgaggccgc ggtcctcttc tcgaaggccg aggggatcat
     7141 cccggccccc gaatccgcgc acgcgatccg cgcggcgatc gacgaggcgc tccgctgcaa
     7201 ggcggcgggg gagaggaagg tcatcgcctt caacctctcg ggccacgggc acttcgacat
     7261 gaccgcctac cagagcttcc acgccggcga gctcaaggac ttcgaatacc ccgacgacgc
     7321 cgtcgaggag gcgatgtccc acctgccgaa ggtggccggc ggctgacccg ggatcgaaac
     7381 cttccggccg cgattgcgtt cgatcgatat gatcagcccg accatgcgaa ggggtgcgtt
     7441 cgcggccgcc ggtatcgccg gtgctgccct cctggcggtc ctcgtcccgc tctacgacgc
     7501 tccggaaccc cggggcttga cggtcgcgcg gggaaaggtc cgcgacgtgg cggacgccga
     7561 ggcccggagg tgggggatcg aggtcgacaa ggcgttcgtc gtgacgacgc tggacaactc
     7621 ccccctcctg gacaaggaac tccgtgacaa gcccgaactc cggcggaggg ccgagtccga
     7681 ccccgtgctc gggccgcgca tcttcgggtt ccgcgtcacg tactggcgca acggcgtcga
     7741 caagtaccct ccctatggct tcgtcaccgt gtcgcgggcg ggagacgtcg tcgccgcgat
     7801 gcgacaggcc cgcctcgagg agacgggggc gtcgccgaag gcggacgacc tacgggcgaa
     7861 ggccgacgcg ttcgtcgcgt cgcgggcctt cgcgggggcg ccgagccccg tcttcgagga
     7921 cgtgcggccg aacgtccaga ggaaccgcgc ggaccacacg ttccggtacc gggtgccgtc
     7981 ctcgttcccg acgggcgacg tcgtctgcta cctctacgtc accttcatcg gcgagaaacc
     8041 cgcgggctgg tcgctcatcg aggagtaccg ggacggccgg cgcttcgcgt acgacgccgg
     8101 catcggcgag acgttcctga ggttcgccgg gatcttctcc ctcctcgccg tcctcctcgc
     8161 cgtcttcctg aggaagtacc acgcgggcga ggtcggggtc gggaccggcg ccgccctctt
     8221 cgcggcggcg atcgggctct cgatcgtcca caacctgctg acggcgccgt gggcggcctc
     8281 cggcaccggc ctcgggtcga tcgacaaccg gacgacgacg ctcgcggttt cgggcttccg
     8341 cttcctcttc tacgacatcc cgctttcgct tctcgtcttc ctcgcgtggt cggtcgggga
     8401 gagcttcgcc cgcgagcgct ggggcgagcg cctcgcgtcc ttcgacgcgc tcctccggcg
     8461 cgacgtgcgc aatgcgaccg tcggcggctc gcttctcgcc ggcctcctcg ccgcgcccgc
     8521 cgtcgccgcg gcggcgctcc tcgtcccggc gctccccttc cgcgcgggcg
//
LOCUS       DAOMFY010000158         8473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2581_length_8473_cov_8.738774, whole genome shotgun sequence.
ACCESSION   DAOMFY010000158 DAOMFY010000000
VERSION     DAOMFY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2581_length_8473_cov_8.738774"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5102)
                     /locus_tag="PLB01_12620"
     CDS             complement(<1..5102)
                     /locus_tag="PLB01_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQQ78190.1"
                     /translation="MRHLKTCGAIVLAIGVAGGPAESAQNIVQPWETLPSFKPNQVFH
                     SGPIDDVSLFSGDAQISVPLGPEFTLSPGVKWQLSARYSSKMWHMWSQDCNAVGCTTF
                     LSRARLGGNSALGAGWTLELGHIIAGSSVQVEDPRPRYRSPAGGITFISMPAGGGYGT
                     PDDNSFVRFSSQPFWTMQRPDGTTWEFTHSFYAPSSPNNYDYCDDDRVNSGIPRYGLE
                     RVRDRFNNVVLTVTYDAQKPWQVKNVLLSNGQSIDFTWGTFQRQGSSYAWPVLSTISF
                     SSVGGNPLTVTFSRFPDGRIGRSPFEQGSSNWCPSDPTRSTIDGPLLSRIDVGGHFYD
                     FTYAANEGTGYETLVSTYTLPTGGRVEYDWLRYTVLKPCVEGNQGCNPESDSALQQAV
                     TQDTGDSCSSWRMYLQNFIDASPSVISRREIDPFGGLTRTTSYSRKQFGGKAQAGPLG
                     DFDVSLVMRQAIVVRPDGNGGSISSKHLFNHDGAELERRHYPTGAATGAPLRSEIFCY
                     DGTNPAGKVCGAIDASRQEPQYYSKFVDLWAVIPIRRSREVTWYGTNPQPGGSCLDNE
                     SIPCRFISRSEWIEGAREFRVERIGLEYTTPSYSSAMAQPGFLRRETTTNWTPARVSP
                     AWMPKIADSVTVQDVHCQSEPCNSVTFGYCPSELAPCDHSKSLTYDTSGNLTFLSISD
                     TRTGYAASVSAGYTFTGNDPTTETVTGSGSVTGSLSTTRTYQGPNLHQVLTAKRNPFT
                     FFSFDVDREAPTGLITASRDANSALVTTYTYDNLGRLKKIEAPGELKTTICYKDPSGS
                     TPAYALVKRTSSDTESVWCDPDAGQPVEGGGPVEAYGYDGFGRLIREVRKLPNLIGGG
                     SLAFRATLYNSAGYVSDETEWQPCGTNQVGTCMTVVPASGKRATYSNFDFKGRWRYRV
                     AADSSSVDRSFDDPAPIWNSDTAELVTLHEVNAAAGSGSGSLAYTAKRSDFLNRLFVT
                     SLPGDTLNPVNPPGFSFRAGPYTSYLWNTLDRVSKTTQESQGRRFDYDKLGNLRSETH
                     PESGATTYSSFDVLGNVLSRTEADGRVVAATYDNLGRLVTVDANSLNYRHNYYDGSGF
                     SGGIYKLGRLTRQVSSNPGSLLENATVEEDYSFSGLGGRLSARTTRLKRSSQAVLLEP
                     EQSFFWNNLGLLQTHVHARVSGSFSTDTYYTSGLPTRLVNGSQTIVSGATYSAFGGLQ
                     SYTTGDGVQTIFTPDATGMSRPSAITTSGVVPAAANFNTGTISYDGVGNIRQMQLSPS
                     GPTDSFTYDLAGRLRKATWGTLGSGINDDFVYDNDLSGLHTSGNITRKGVDVTCTLVL
                     GDPCTPLDVNAATNRLTTGTYGPSGEIRTFGPKTYAYDGLLRQVRFSQSGSVEHSLFG
                     ADDERLARVVPTAAVPETVKATRLFTLSPCRLYDTRNSTALVPGVERTVQASGICGVP
                     SNATALAANVTSINASAENGSMRAYPAGISFRPLMTANAIRGGRTRGMMNIIELGDPN
                     GTEPGKFRLLSDMPSASLHALVDVSGYFAPVSTATGNSWYVTLRDNDHRLSTEYRWDE
                     SGNVMNLLSDHIYLGSIRVATVGTSATEGVPLGLTYYSTDHLGTPRLAHTTGGQLVAS
                     YRYRSFGEPYTGSGQTPGTATTQGLGFALMERDALSGDHYDHARFYSGYTGRFNSPDV
                     LRGRVEDPQSWNRYAYARNNPLKYVDPDGRSA"
     gene            complement(5214..6518)
                     /locus_tag="PLB01_12625"
     CDS             complement(5214..6518)
                     /locus_tag="PLB01_12625"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQQ78191.1"
                     /translation="MKSDSPAPVRVGLLGWGNVGRAFARLLASRAPDLADRRGVSLRL
                     VAVGTRRPDEAREAGLGDVRWTTDLASVATAPDVDLVVELIGGHEPANALIRAALGGG
                     KSVVTANKLLLAKEGASLQALAREKKAGLGVEAAVAGGIPILRALRESFAGDRVASVS
                     GILNGTCNFILTEMEATGRAYVDVLKDAQRLGYAEADPASDVEGEDAAYKLALLARLA
                     FGRDAQVAQIAREGITRLLPCDFLYAKRLGRTPRQLGVARELPSGHLLLSVRTHLVSS
                     ASLLAKVTGPFNAIEVKAAAGGSFVFSGRGAGGEPTATAVLADVVELVRSRGEPRVPP
                     LGFADLLPCEAASAGEFVSAYSLRFVVRDRSGIIADLARILADHGANIDAVFQEPWPD
                     KDALPFVITLEPVAQSALDSALAKIRGLDFHVEPPLALPLTN"
     gene            6578..8128
                     /locus_tag="PLB01_12630"
     CDS             6578..8128
                     /locus_tag="PLB01_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HQQ78192.1"
                     /translation="MLTTLALTVLLAQTRAARPEATPAATPAASAKADEAAQKDDKKK
                     DEKEEPPVVTKHTLTAGGKTLSYAATTGFLPLKTDAGETEAKVFFMAYTLDRGGAPAS
                     KRPLTFSFNGGPGAASVWLHMGALGPRRVKMKDADGMMPAPPYTLVDNEETWLGDTDM
                     VFIDPVGTGYSRPAKPELGKKFWGLDGDIASVGEFIRLYLTRYERWGSPLFLVGESYG
                     TTRAAGLSGYLVDRGIAFNGILLVSSILNFQTARFTRGNDLPYPLFLPTYTATAWYHK
                     KLPADLQGKPLRAVLDEVEAFAGGEYPVALAKGDALPPAERKALAARLARYTGLDAGW
                     IERADERIEIMHFCRELLAAERKNVGRIDSRFTGDNESGIAERPDFDPSIAAIVPPYT
                     AAFNQYVRADLGFKSDLTYYVLGGGIGSPWEFPQNTFADTSGALRSAFAKNPSMRLFV
                     ASGHYDLATPHFATRYTLSHMGLTAEQRARITAKEYEAGHMMYLHVGELAKLRKDVGA
                     FFASALGK"
     gene            8125..>8473
                     /locus_tag="PLB01_12635"
     CDS             8125..>8473
                     /locus_tag="PLB01_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HQQ78193.1"
                     /translation="MTGSALALILAFPVGLSLGLLGGGGSILTVPVLVYAAGLDPKVA
                     IPASLVSVGTTSLVAALAHARQKAVAWGAALAFGGAGLVGAFLGARAGRGFSGRTLLV
                     LFSRDAGDLGESYV"
BASE COUNT         1427 a         2782 c         2781 g         1483 t
ORIGIN      
        1 ttcgctgaac gcccgtccgg gtccacgtac ttgagtggat tatttctagc gtaggcatat
       61 cgattccagc tctgtggatc ttcaaccctt ccacgcaaga cgtccgggct gttgaatcga
      121 cccgtatagc ccgagtagaa cctcgcgtgg tcgtagtggt cgccactgag ggcgtccctt
      181 tccatcaagg cgaatcccag gccctgggtc gtcgccgttc caggggtctg cccgctgccc
      241 gtgtaaggct ctccgaacga cctgtatcgg tagctcgcga ccagctgccc acccgtcgtg
      301 tgtgcgaggc ggggggtgcc gagatggtcg gttgagtagt acgtgagccc aagcgggacc
      361 ccctcggtcg cggaggtgcc cacggtcgca acccgaatgc tgccgaggta gatgtgatcg
      421 gacagcaggt tcatgacgtt cccgctttcg tcccaccggt actcggtcga gagacgatga
      481 tcgttgtcgc gcagcgtcac gtaccaggag tttccggtcg ctgtcgagac cggcgcaaag
      541 tagccgctta cgtccacgag cgcatgcagg ctcgcgctcg gcatgtccga gagcagccgg
      601 aacttccctg gctcggtgcc atttggatct ccaagctcga tgatgttcat catcccacgc
      661 gtgcggcccc cgcggatcgc attcgcggtc ataaggggac ggaaagagat tcccgccggg
      721 taggcacgca tcgagccgtt ctctgccgat gcgttgatgg atgtgacgtt tgcggcgagc
      781 gccgtcgcat tcgagggcac gccgcagatt ccgctcgcct gcacggtgcg ctcgacgccg
      841 ggaacgagcg cagtcgagtt cctcgtgtcg tagagacggc aagggctgag tgtgaagagc
      901 cgggtcgcct tcactgtctc ggggacggcc gccgtcggga cgactcgtgc gagtctctcg
      961 tcgtccgcgc cgaacagcga gtgttcgacc gaacccgatt gtgagaacct cacctggcgc
     1021 aggaggccgt cgtaggcata cgtcttgggt ccgaaggtcc ggatctcacc cgatggcccg
     1081 taggttccag tcgtcagcct attcgtcgca gcattcacgt ccagaggcgt gcacggatcg
     1141 ccgagaacca gggtgcacgt gacgtcaacc cccttccggg tgatgttgcc cgatgtgtga
     1201 aggcctgaaa ggtcattgtc gtagacgaaa tcatcgttga tccccgagcc caaggtgccc
     1261 catgttgcct ttcgcagccg cccggccagg tcgtatgtga acgaatccgt cggacccgaa
     1321 ggcgagagct gcatctggcg aatgttgccc actccatcgt aggagatcgt gcccgtgttg
     1381 aagtttgcgg ccgcagggac gacccccgac gtcgtgatcg cgctcggacg ggacataccc
     1441 gtcgcatcgg gcgtgaagat cgtctggacc ccgtctcccg ttgtatacga ctggagcccg
     1501 ccgaaggcgc tgtatgtggc gcccgacacg atggtctgcg acccgttaac gaggcgcgtc
     1561 ggaagccccg acgtgtagta ggtgtccgtg gagaaagacc ccgagacacg ggcatgcacg
     1621 tgggtctgta acaggccaag gttattccag aagaaagact gttctggctc caacaggact
     1681 gcctgagagg atctcttgag gcgagtcgtc cgtgccgaga gtcggcctcc gaggccggag
     1741 aacgaatagt cctcttcgac cgtggcgttc tccagcagtg agccggggtt cgacgaaacc
     1801 tggcgggtga ggcgacccag cttatagatg cctccactga agccgctccc gtcatagtag
     1861 ttatgccggt agttgagcga gttcgcgtcg accgtcacga ggcgtccgag attgtcgtat
     1921 gtcgccgcga caacccggcc gtctgcctcc gtcctcgaaa gcacgtttcc gaggacgtcg
     1981 aacgacgaat aggtcgtggc gcctgattcc ggatgcgttt cagaacgcag gtttcccagc
     2041 ttgtcgtagt cgaaccttcg gccttggctt tcctgagtgg ttttggagac cctgtcgaga
     2101 gtgttccaca gatacgacgt gtaggggccg gccctgaatg agaagcccgg cgggttcacg
     2161 gggttgaggg tgtctccggg gaggctcgtc acgaacaaac ggttcagaaa atccgatcgt
     2221 ttggcggtgt atgcaagtga acctgagccg cttccggctg ccgcgttcac ctcgtgaagg
     2281 gtcacgagct ccgcagtgtc ggaattccag attggggcag gatcgtcgaa agaacgatcg
     2341 acgctcgagg aatccgccgc aacgcggtac ctccagcgcc ccttgaagtc gaagttcgag
     2401 tacgttgccc gcttccccga ggccggaaca actgtcatgc aggtcccgac ctggtttgtc
     2461 ccacagggct gccactccgt ctcgtccgaa acatatcctg cgctgttata gagggtagct
     2521 cgaaaagcaa gacttccgcc gccgatgagg ttcggcagct ttctcacctc gcggatcagg
     2581 cgaccgaagc cgtcgtatcc ataggcctcg accggccctc ccccttcaac tggttgaccg
     2641 gcatcggggt cgcaccagac tgactcggtg tccgaggatg tgcgtttgac gagcgcatac
     2701 gcgggcgtcg agccggaggg gtccttgtag cagatggtcg tcttcagctc cccaggcgcc
     2761 tcgatcttct tgaggcggcc cagattgtcg taggtgtagg tcgtcacgag tgcgctgttc
     2821 gcgtcgcgac tcgcggttat caggccggta ggtgcttccc gatccacgtc gaaggagaag
     2881 aacgtgaaag gattccgttt ggccgtgagc acctggtgga ggttcgggcc ttgataggtc
     2941 ctcgttgtac tcagcgatcc agtcaccgat cccgatcccg tcacggtctc ggtcgtcggg
     3001 tcgttgcccg tgaaagtgta gccggccgag acggatgccg catacccagt ccgcgtgtcg
     3061 gagatcgaaa ggaaggtgag gttgccgctc gtatcgtagg tcagcgactt cgagtggtcg
     3121 cacggtgcca gttcgctcgg gcaatagcca aacgtcaccg aattgcatgg ctcgctctga
     3181 cagtgcacgt cctggaccgt gacggaatcg gcgatcttcg gcatccatgc cggcgagact
     3241 cgtgccggcg tccagtttgt cgtggtctcc cttcgaagaa agccgggctg ggccatggcc
     3301 gagctgtaac tgggcgtcgt gtattcgagc ccgattctct cgacgcgaaa ctcgcgtgcg
     3361 ccttcgatcc attcgctgcg cgagatgaat cggcacggaa tcgattcgtt gtccaggcag
     3421 ctgccgccgg gttgcggatt cgtcccgtac caagtcactt cccgcgagcg tcggattgga
     3481 atcaccgccc acagatcaac aaacttgctg tagtactgcg gctcttggcg gctggcatcg
     3541 atggctccac agaccttccc agcgggattc gtgccgtcgt aacagaagat ctcggacctc
     3601 aaaggcgcgc ctgtcgcggc gcccgtcggg taatggcggc gctccagctc ggcaccgtcg
     3661 tggttgaaga ggtgcttcga agagatggag cctccgtttc catcaggacg tacgactatg
     3721 gcctggcgca tgaccagcga aacgtcgaaa tctcccaagg gccccgcctg ggccttccct
     3781 ccgaactgct tccttgagta actcgtcgtc cgagtcaaac ccccaaaggg gtcaatctcg
     3841 cggcgcgata tgacactggg gctcgcgtcg atgaaattct ggaggtacat tcgccaggaa
     3901 gaacacgagt cgccggtgtc ctgcgtcacg gcctgctgca gcgcggagtc ggattccggg
     3961 ttgcaccctt ggttcccctc tacgcagggc ttcaggaccg tgtagcggag ccagtcgtat
     4021 tccacacggc cgcctgtcgg cagggtgtag gttgagacta gagtctcgta gcccgtgcct
     4081 tcgttcgccg cgtaggtgaa gtcgtagaag tggccgccga cgtcaatgcg gctgagtagt
     4141 ggcccgtcga tcgttgatct cgtcgggtcc gatgggcacc aattcgagct gccctgctcg
     4201 aacggcgacc gaccgatcct gccatccgga aaacgcgaga aggtcactgt cagcgggttt
     4261 ccacccacgg acgaaaagct gatggtgctg aggactggcc aggcatacga gctaccctgg
     4321 cgctggaacg tgccccaggt gaagtcgata gactggccgt tcgagagcag gacgttcttg
     4381 acctgccacg gcttctgggc gtcgtaggtc accgtgagga cgacgttgtt gaagcggtcg
     4441 cggacgcgct ccagcccata ccgagggatt cccgagttca cgcggtcgtc gtcacaatag
     4501 tcgtagttgt tcggcgatga cggcgcgtag aagctgtgcg tgaattccca cgtcgtcccg
     4561 tccgggcgct gcatcgtcca gaacggctgt gacgagaacc tgacgaagct gttgtcgtcg
     4621 ggcgtgccgt atccgccgcc agcaggcatc gaaatgaaag tgataccgcc agccggtgag
     4681 cgatagcggg gccgcgggtc ttcgacctgg acgctgctgc ccgcgatgat gtgcccgagt
     4741 tccagcgtcc atcccgcccc cagcgcggag ttgccgccga gacgcgcacg ggacagaaac
     4801 gtcgtgcagc cgactgcgtt gcaatcctgg ctccacatat gccacatttt gctcgagtag
     4861 cgcgctgaga gctgccactt gacccccgga ctcagcgtaa attcggggcc gagcggtacg
     4921 gagatctgag cgtctccgct aaagaggctg acgtcgtcaa tgggaccgga atgaaagacc
     4981 tggttgggct tgaatgaagg aagtgtctcc cagggctgga cgatgttctg agccgattcg
     5041 gccggaccgc cggccacgcc gatggcaagc acgatcgcac cgcaagtctt caagtgtctc
     5101 aaaacgccct ctccgcgcct cgcctgaccg agagcgaggc gtcccctatc cctatagaca
     5161 gttcagggcg ggtttttcga cctcccccaa gaggaaaagg ctcttcctgg ggtctagttc
     5221 gtcaacggca gcgcgagcgg gggctcgacg tggaagtcga gcccccggat cttcgcgagc
     5281 gccgagtcga gcgcgctctg cgcgaccggc tcgagcgtga tgacgaacgg gagcgcgtcc
     5341 ttgtcgggcc acggctcctg gaagaccgcg tcgatgttcg cgccgtggtc cgcgaggatc
     5401 cgcgcgaggt ccgcgatgat gccggaccgg tcgcgcacga cgaaccgcag cgagtaggcc
     5461 gacacgaatt cacccgccga agccgcctcg cacgggagaa ggtccgcgaa gccgagcggc
     5521 gggacgcgcg gctcgcccct ggaccggacc agctccacga cgtccgcgag gacggccgtc
     5581 gccgtcggtt cgccgcccgc gccgcggccc gagaacacga acgatccgcc cgccgccgcc
     5641 ttcacctcga tcgcgttgaa cggccccgtc accttcgcga ggagcgacgc gctcgagacg
     5701 aggtgcgtcc tcacggacag aagcaggtgg cccgagggca gctcgcgcgc gacgccgagc
     5761 tggcgcggcg tccgcccgag gcgcttcgcg tagaggaagt cgcacgggag aagacgcgtg
     5821 atgccttcgc gcgcgatctg cgcgacctgc gcgtcgcgcc cgaacgcgag gcgcgcgagg
     5881 agtgcgagct tgtaggccgc gtcctcgccc tcgacgtcgc tcgccgggtc ggcctcggcg
     5941 tacccgagcc gctgggcgtc cttcaggacg tcgacgtagg cgcggcccgt cgcctccatc
     6001 tccgtgagga tgaagttgca ggtcccgttc aggatccccg acacggaggc gacccggtcg
     6061 cccgcgaagc tctcgcgcag ggcgcggagg atcggaatgc cgcccgcgac ggccgcctcg
     6121 acgccgagcc cggccttctt ctcccgcgcg agcgcctgca ggctcgcgcc ttccttcgcg
     6181 aggaggagct tgttcgccgt gacgacgctc ttcccgccgc cgagcgccgc gcggatgagc
     6241 gcgttcgcgg gctcgtgccc gccgatcagc tccacgacga ggtccacgtc cggcgccgtc
     6301 gcgaccgacg cgaggtccgt cgtccagcgc acgtcgccga gtcccgcctc gcgcgcctcg
     6361 tccggccggc gcgtccccac cgcgacgagc cgaaggctca cgccgcggcg gtccgcgaga
     6421 tccggcgcgc gggaggcgag gaggcgggcg aacgcgcgtc cgacgtttcc ccagccgagg
     6481 agacccacgc ggaccggcgc cggactatcg cttttcatgc ccgggcaatg tacggtacgg
     6541 ccaccccgcg gtcaaagaac gcctccggag gctttccatg ctgacgacgc tcgccctcac
     6601 cgtcctcctc gcccagacgc gcgcggcgcg gcccgaagcc accccggccg cgacgcccgc
     6661 cgcctcggcg aaggccgacg aagccgccca gaaggacgac aagaagaagg acgagaagga
     6721 agagccgccg gtcgtcacga agcacacgct gacggcgggc ggaaagacgc tctcctacgc
     6781 ggccacgacg ggctttctgc cgctcaagac cgacgccggc gagaccgagg cgaaggtctt
     6841 cttcatggcg tacacgctcg accgcggcgg ggcccccgcg tcgaagcggc cgctcacgtt
     6901 ctcgttcaac ggcggccccg gcgccgcgtc cgtgtggctt cacatgggag cgctcgggcc
     6961 caggcgcgtg aagatgaagg acgccgacgg gatgatgccc gcgccgccgt acaccctcgt
     7021 cgacaacgag gagacgtggc tcggggacac cgacatggtc ttcatcgacc ccgtcggcac
     7081 cggctacagc cggcccgcca agccggagct cgggaagaag ttctggggcc tcgacggcga
     7141 catcgcgtcc gtgggcgagt tcatccgcct gtacctcacg cgctacgagc gctggggctc
     7201 gccgctcttc ctcgtgggcg agagctacgg gacgacgcgc gcggccggcc tttcgggcta
     7261 cctcgtggac cgcggaatcg cgttcaacgg catcctcctc gtgtcctcga tcctgaactt
     7321 ccagacggcc cgcttcacgc gcggcaacga cctcccgtac ccgctcttcc tcccgacgta
     7381 cacggcgacc gcctggtatc acaagaagct ccccgccgac ctgcagggca agccgctgcg
     7441 cgccgtcctc gacgaggtgg aggcgttcgc gggcggcgag tatcccgtcg cgctcgcgaa
     7501 gggcgacgcg ctgccgcccg ccgagcgcaa ggcgctcgcg gcgcgcctcg cgcgctacac
     7561 gggcctcgac gccgggtgga tcgagcgggc ggacgagcgg atcgagatca tgcacttctg
     7621 ccgggagctc ctcgcggccg agaggaagaa cgtcgggcgc atcgactccc gcttcacggg
     7681 cgacaacgag tccggcatcg ccgagcgccc cgacttcgac ccgagcatcg cggcgatcgt
     7741 cccgccctac accgccgcct tcaaccagta cgtgcgggcc gacctcggct tcaagagcga
     7801 cctgacgtac tacgtgctcg gcggcggcat cgggagcccg tgggagttcc ctcagaacac
     7861 gttcgcggac acgagcggcg ccctgcgctc ggccttcgcg aagaacccct ccatgcgcct
     7921 cttcgtcgcg tcgggacact acgacctcgc gacgccccac ttcgcgacgc gctacacgct
     7981 ctcgcacatg ggcctcacgg ccgagcagcg cgcgcggatc acggcgaagg agtacgaggc
     8041 ggggcacatg atgtatctcc acgtcggcga actcgcgaag ctccgcaagg acgtcggggc
     8101 gttcttcgcg tcggcgctcg ggaagtgacc gggagcgctc tcgcgctgat cctcgccttt
     8161 ccggtcgggc tctcgctcgg cctcctcggc ggcggcgggt cgatcctgac cgtgccggtc
     8221 ctcgtgtacg ccgccggact cgatccgaag gtcgcgatcc ccgcctcgct cgtgagcgtc
     8281 gggacgacga gcctcgtcgc cgcgctcgcg cacgcgcgac agaaggcggt cgcgtggggc
     8341 gcggcgctcg cgttcggcgg agccggcctc gtcggcgcgt tcctcggcgc gcgtgcgggc
     8401 cggggcttct cggggcgcac gcttctggtg ctgttctcga gagacgccgg ggatctcggc
     8461 gaatcctacg tcc
//
LOCUS       DAOMFY010000159         8466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2588_length_8466_cov_8.668886, whole genome shotgun sequence.
ACCESSION   DAOMFY010000159 DAOMFY010000000
VERSION     DAOMFY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_8466_cov_8.668886"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLB01_12640"
     CDS             <1..368
                     /locus_tag="PLB01_12640"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQQ78194.1"
                     /translation="RPAAAAAPLREGPLRRVVVKSGTTVTILPVETIDFVKAEDDYVL
                     LSAGGRKHLKQQTLASLEDALPKERFVRVHRSYLLNLDRLKRVEPSETGSPTAVLADG
                     TRLPVSRAGAQRLSEILGR"
     gene            397..1074
                     /locus_tag="PLB01_12645"
     CDS             397..1074
                     /locus_tag="PLB01_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78195.1"
                     /translation="MIWPFGKKAREGWRDKPQAITSAIPRDDDRAGPVPPPPRTAHAP
                     GVAIHGYSEVPSQHLLRLDTGHAGFFLEGEEGKDRSSALLVLEPGGATTPGDVLLWSS
                     PLSLSLEEISRTSPRPSHHIRRDDLSGRGVSGITGLRSRHEGGGITVLRFGPPPGARW
                     VFVGLRAIAVFAGKVTLVDGEESKVVPAGRIALVGDPTATLYVEAGNDSALAVALGGA
                     RVVSALG"
     gene            1151..>1917
                     /locus_tag="PLB01_12650"
     CDS             1151..>1917
                     /locus_tag="PLB01_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ78196.1"
                     /translation="MSPSKPAAEPPMSAKRGADLMPTVNPKGEMAWESSPSRYIAKVS
                     LSTGKEILRPIDPDELLVSAHRDKNPIEVTAQFFASRAISWTGNEQTVKDGLRKVNLL
                     GLKIAEALGQATGSRAVETQAKKLIGVSERNVFFKFREASHLTEKEVKARVEKLQKDA
                     RALLRARGRSPKLHVLLTGATGFLGKEILFQAATDRRIERVVCVVRPEKIRDPKTKEV
                     VKVLSPKQRGAILLKRIGISGAKAKKFVFLDGDIEQPR"
     assembly_gap    1918..2017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2018..2923
                     /locus_tag="PLB01_12655"
     CDS             <2018..2923
                     /locus_tag="PLB01_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78197.1"
                     /translation="AQESALVFPRNFYNNFYELTKAMASLETDYTLIEKGLRVSQLLP
                     SIVIGHSKTGNNRGDTKVVNAPINAFGRSKEAMDALGADTVGRGKAWLIGSIAAAFPG
                     DRSAELNIVPVDRVVEGILAALTVPESIGERIHLATDNRIRSEDIARITREEIGIDVH
                     LADPTLFRNVTLPVVKAVLERSGEPKLANALDKLGTIFGGYGEWGQPIHDVGNDVRIL
                     GLPLRRPNTENAFRMLCRHNKYVQEFGKVRDLDEIARREHLWEKTLKEIEHKTGRQVA
                     SIPPDEFRRLLSQRLELKGFREREK"
     gene            complement(2933..3718)
                     /locus_tag="PLB01_12660"
     CDS             complement(2933..3718)
                     /locus_tag="PLB01_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQQ78198.1"
                     /translation="MTIRIALAQPPYPATPEQSIRIVEELIEGASKDGAKVVCFPECY
                     VPGYRGLGRDPGPPDAAFLDDAHARVAAAARRSNIGVVLGTERLQGRTLYATALVFGP
                     DGSVLGRQDKVQLDPSEDATYTPGAGRRVFTVGALTFGVAICHEGWRYPETVRAAARG
                     GAQVVFHPHLELGGPGGYAPKEFGDPKNTFHEKALLCRAAENTIWIASVNYAAEGSAT
                     TSAFVRPDGTLLAFQPYGAEGLLVADLDLSLATGLLAKRFRPV"
     gene            complement(3715..5325)
                     /locus_tag="PLB01_12665"
     CDS             complement(3715..5325)
                     /locus_tag="PLB01_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQQ78199.1"
                     /translation="MKRLLEFLESRAGASSLWRAFADEPVSVRRAWLFTLGSAALFAL
                     VVQVVTGLALALSYAPTPDHAWESVKAIQTKLPAGAVVRGLHHWGASAMVVLAALHLA
                     RTFLFGAYRKPRELNWLVGVALLLLILAFGFTGYLLPWDQKAYWATVVGTKVPAALPV
                     VGPAASRVMAGGNVVGAATLTRFYAVHVILLPILTLGLVALHLLLLRKHGHAGPTAEP
                     DPRQPFFPYQAARDAVVGLAVVLLLFALAAASPAPLERLADPADTSYVPRPEWYFLPL
                     FQLLKFFQGPLEPIGTAVLPGVSMLLLALVPWLDRGASRRPGDRKPVLVLGALVAAGI
                     LALLVFGALDAPKVKPRSGLGASDVPDPRLLGGLASYERRQCASCHGVEGVLAPAAPA
                     GSVPLAMRSHALAGDALVAHVKDKTPKPANAEEGGEDPDKDVPSLLEYLGALREGAAF
                     AALPAPIRLGGAVIDREDCRQCHQVYGDGGRRGPALQHLLGKRDKAWLVEHFKDPKKL
                     VEGSKMPKYAYLSEDELAAMAEYLLALP"
     gene            complement(5322..5819)
                     /locus_tag="PLB01_12670"
     CDS             complement(5322..5819)
                     /locus_tag="PLB01_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQQ78200.1"
                     /translation="MPSRRAFTFTALAAGAAAFASVAGAALSFALSPLVRRASAGGAA
                     LDLGAAKDFEAVKAGAAGAAEVVVLRTVEDGYMTRRVKERLAVVRDASSPSGLAAVST
                     TCTHLGCGVAWNPEKKAFLCPCHGGVYGADGRVLAGPPPRPLPKAALKVAGGRVTVDP
                     AAFEA"
     gene            complement(5840..6712)
                     /locus_tag="PLB01_12675"
     CDS             complement(5840..6712)
                     /locus_tag="PLB01_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HQQ78201.1"
                     /translation="MSATPTLRALRPAQWVKNLFVLAPVLFGKVADRGELAGRTFAVA
                     VAFCALASALYLLNDVRDAEADRQHPVKKDRPVASGALPPGTALVLAAVLGPLALLAV
                     LFAAPAALPATLVYAGVTVLYSLGLKKVALLDVFIVASGYVLRVLAGSAAAQVKPSHW
                     LLLCTFFLALFLALSKRRSELAARGAAARPSLKEVPVGLLEAFENITLGTTIVCYALY
                     TVSPETIAWFRTDRLLVTVPIVLFGLFRWRLLEARGAGEDATKDLFTDAGLLATVVIW
                     GTVCAALIYGPGRT"
     gene            6754..7647
                     /locus_tag="PLB01_12680"
     CDS             6754..7647
                     /locus_tag="PLB01_12680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ78202.1"
                     /translation="MPAPDAPLRLESCACPLCGEAPPARARVSFPPYRVVECPRCTLR
                     YLSPRVAREDLGRLYEGGYWEGGGTEGGYASYAEMEALLAKTFARRLRWLGPPPPGGR
                     LLDVGCGPGAGLDAARAAGWEAWGLDLSSEAVARASARHPGRVAQGTLEDSPFPAASF
                     DAITAFDLVEHVYEPRRFATALAAALKPGGSVLIATPNVESLLARATGRRWVSYKIPE
                     HVTFFSRRTLADALAPPLTLVRAAPCGQHVSLPFLLERVGAALPAGGGLLRTAARGRT
                     LSGLTLYANSGSMLVRAVRTT"
     gene            7653..8429
                     /locus_tag="PLB01_12685"
     CDS             7653..8429
                     /locus_tag="PLB01_12685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ78203.1"
                     /translation="MDVSVVLPVYNGAAFVAESVSGLAAFLERQAFSWEIVVVDDGSR
                     DATAHEVPDGTGIHLLRLAENRGKFGALKAGMAAATGRCRVFTDADVPYDLEALPYVA
                     SLVNDRGFHVVVGDRSLTDSIYGSRLPRSRAAASRAFSLLVRLLVTGGLFDTQCGLKG
                     FRGDVADALFPLLTVEGFAGDVEVLYVALKYNLSIRRIPVRLRRHERSTIRFGAHAPA
                     MLRDALLLRGKWDRGLYASAALARIAAQDYWVPPAPETAA"
BASE COUNT         1279 a         2979 c         3000 g         1108 t
ORIGIN      
        1 cccgcccggc ggcagcggcc gcgccgctcc gcgaggggcc gctccggcgc gtcgtcgtga
       61 agtccggcac gacggtgacc atccttcccg tcgagacgat cgacttcgtg aaggccgagg
      121 acgactacgt gctcctctcc gcgggaggga ggaaacacct caagcagcag acgctcgcga
      181 gcctcgagga cgcgcttccg aaggagcgct tcgtccgtgt ccaccgctcg tacctcctga
      241 acctcgaccg gctcaagagg gtcgagccct cggagacggg ctcacccacg gcggtcctcg
      301 ccgacggcac gcgcctgccc gtgagccgcg cgggcgccca gcgcctgagc gaaatcctcg
      361 ggcgttgagg agcgggagtc cgttagagtc cccgggatga tctggccgtt cgggaagaag
      421 gcgcgggagg gctggcgcga caagccgcag gcgatcacga gcgcgatccc gcgcgacgac
      481 gaccgcgccg gccccgtccc gcctccgccg cgcacggcgc acgcgccggg cgtggcgatc
      541 catggttatt ccgaagttcc ctctcagcac ctgttgcgtc tcgacacggg ccatgctggg
      601 ttcttcttag aaggtgaaga ggggaaggat cgttcgtcgg cgctgctggt gctcgaaccc
      661 ggcggcgcga ccacccccgg cgacgtcctg ctctggtctt ctcctctttc tctttcactc
      721 gaagaaattt ccagaacatc cccccgtccg agccatcaca tcaggcgcga cgacctctcg
      781 ggtcgcgggg tctccggcat caccggcctg cgctcgcggc acgagggcgg cggaatcacg
      841 gtcctgcgct tcggtccgcc gcccggggcg cgctgggtgt tcgtcggcct ccgcgcgatc
      901 gccgtcttcg ccggcaaggt cacgctcgtc gacggcgagg agtcgaaggt cgtgcccgcc
      961 gggcgcatcg ccctcgtcgg cgacccgacg gccacgctct acgtcgaagc cggcaacgac
     1021 tcggcgctcg ccgtcgcact cggcggggct cgcgtcgtct cggcgctcgg ctgacgtcgc
     1081 gtaaacacgg cgcggcttct gtccaaccgg cgcgcgtcta gaatggtccc cccttccgga
     1141 ggacccgccc atgtcaccgt cgaaacccgc cgccgagccg cccatgtccg ccaagcgcgg
     1201 agccgacctc atgccgaccg tcaaccccaa gggcgagatg gcgtgggaga gctctccgtc
     1261 gcgttacatc gccaaggtga gcctctcgac gggcaaggag atcctccgcc cgatcgaccc
     1321 cgacgagctc ctcgtctcgg cgcaccgcga caagaatccg atcgaggtga ccgcgcagtt
     1381 cttcgcgagc cgcgcgatct cgtggaccgg caacgagcag acggtcaagg acggcctgcg
     1441 aaaggtgaac ctcctcgggc tgaagatcgc cgaggcgctc ggacaggcca cgggcagccg
     1501 cgccgtcgag acgcaggcca agaagctcat cggagtctcc gagcgcaacg tgttcttcaa
     1561 gttccgcgag gcctcccacc tcaccgagaa ggaggtcaag gcgcgcgtcg agaagctgca
     1621 gaaggacgcg cgggcccttc tgcgcgcccg cgggcgcagc ccgaagcttc acgtcctcct
     1681 gacgggcgcg acgggcttcc tcggcaagga gatcctcttc caggccgcga ccgaccggcg
     1741 gatcgagcgc gtcgtgtgcg tcgtcaggcc cgagaagatc cgcgacccga agacgaagga
     1801 ggtcgtgaag gtcctctcgc cgaagcagcg cggggcgatc ctcctgaagc gcatcgggat
     1861 ctccggcgcg aaggcgaaga agttcgtctt cctcgacggc gacatcgagc agccgcgnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg caggagtcgg cgctcgtctt
     2041 cccgcggaac ttctacaaca acttctacga gctgacgaag gcgatggcct cgctcgagac
     2101 ggactacacg ctcatcgaga aagggctgcg cgtctcgcag ctcctgccct cgatcgtgat
     2161 cggccacagc aagacgggca acaaccgcgg cgacacgaag gtcgtgaacg cgccgatcaa
     2221 cgcgttcggc cggagcaagg aggcgatgga cgccctcggg gccgacaccg tcgggcgcgg
     2281 gaaggcctgg ctcatcgggt cgatcgcggc cgcgttcccg ggcgaccgct ccgccgagct
     2341 gaacatcgtc cccgtggatc gcgtcgtcga gggcatcctc gcggcgctca ccgtgcccga
     2401 gtccatcggc gagcggatcc acctcgcgac ggacaaccgc atccgctcgg aggacatcgc
     2461 gcggatcacg cgcgaggaga tcgggatcga cgtccacctc gcggacccga cgctcttccg
     2521 caacgtcacg ctgcccgtcg tcaaggcggt cctcgaacgg agcggcgagc cgaagctcgc
     2581 gaacgcgctc gacaagctcg ggacgatctt cggcggctac ggcgagtggg gccagccgat
     2641 ccacgacgtc ggaaacgacg tccgcatcct cggcctcccg ctccggcgcc cgaacacgga
     2701 gaacgcgttc cggatgctct gccgccacaa caagtacgtg caggaattcg gcaaggtccg
     2761 cgacctcgac gagatcgcgc gccgcgagca cctgtgggag aagacgctca aggagatcga
     2821 gcacaagacc ggccgccagg tcgcgtccat cccgcccgac gagttccgcc gcttactctc
     2881 ccagcgcctc gagctgaagg ggttccggga gagggagaag taacgattcc ggctagacgg
     2941 gcctgaagcg cttcgcgaga agccccgtcg cgagcgagag gtcgaggtcg gcgacgagaa
     3001 ggccttccgc gccgtagggc tggaacgcga ggagcgtccc gtcgggcctc acgaacgccg
     3061 acgtcgtcgc ggatccctcc gccgcgtagt tcaccgacgc gatccagatc gtgttctccg
     3121 ccgcgcggca gagcagcgcc ttctcgtgga acgtgttctt tgggtcgccg aactccttcg
     3181 gcgcgtagcc gcccggcccg cccagctcga ggtgcgggtg gaagacgacc tgcgcgccgc
     3241 ctcgcgcggc tgcgcgcacc gtctcgggat accgccagcc ctcgtggcag atggcgacgc
     3301 cgaacgtgag cgcgccgacc gtgaagacgc ggcggcccgc gcccggcgtg tacgtcgcgt
     3361 cctcggacgg atcgagctgc accttgtcct gccgcccgag gacggagccg tcgggcccga
     3421 agaccagagc ggtcgcgtag agcgtgcggc cctggagcct ctccgtgccg aggacgactc
     3481 cgatgttcga ccggcgcgcc gccgcggcga cgcgcgcgtg agcgtcgtcg agaaatgcgg
     3541 cgtctggcgg cccgggatcc cttccgaggc cccgataccc ggggacgtaa cactcgggaa
     3601 agcagacgac cttcgcgccg tccttcgacg cgccttcgat gagctcttcg acgatccgga
     3661 tggattgctc cggggtcgcc gggtacggcg gctgcgcgag cgcgatccga atcgtcacgg
     3721 cagcgcgagg aggtactccg ccatcgcggc gagctcgtcc tcggagaggt aggcgtactt
     3781 cggcatcttc gagccctcga cgagcttctt cgggtccttg aagtgctcga cgagccaggc
     3841 cttgtcgcgc ttgccgagga ggtgctgcag cgcggggccg cggcggcccc cgtcgccgta
     3901 gacctggtgg cactggcggc aatcctcgcg gtcgatcacg gcgccgccga gacggatcgg
     3961 cgcagggagc gccgcgaacg ccgcgccttc cctcagcgcg ccgagatact cgaggagaga
     4021 ggggacgtcc ttgtcgggat cctctccgcc ctcttccgcg ttcgcgggct tcggcgtctt
     4081 gtccttcacg tgcgcgacca gcgcgtcgcc ggcgagcgcg tgcgaacgca tcgcgagcgg
     4141 gaccgacccg gccggcgcgg cgggggcgag cacgccttcg acgccgtggc aggacgcgca
     4201 ctggcgacgc tcgtaggacg cgaggccgcc gagaaggcgc gggtccggaa cgtcggaggc
     4261 gccgaggccg gagcgcggct tgaccttcgg cgcgtcgaga gcgccgaaga cgaggagcgc
     4321 gaggatcccc gcggcgacga gcgcgccgag aacgaggacg ggctttcggt cgcccgggcg
     4381 gcgcgacgca ccacggtcga gccacgggac gagggcgaga agaagcatcg agaccccggg
     4441 gaggaccgcc gtgccgatcg gctcgagcgg cccctggaag aacttcagga gctggaagag
     4501 cgggaggaaa taccactccg gccgcggcac gtacgacgtg tccgccgggt cggcgagccg
     4561 ctcgagcggc gcgggcgacg ccgcggcgag ggcgaagagg agcagcacga ccgcgaggcc
     4621 gacgaccgcg tcccgcgccg cctggtacgg gaagaacggc tgccgcgggt cgggctccgc
     4681 cgtcggcccc gcgtggccgt gcttacggag gagaaggagg tgcagggcga cgaggccgag
     4741 cgtcaggatc gggaggagga tcacgtgcac ggcgtagaag cgcgtgagcg tcgcggcgcc
     4801 gacgacgttt ccacccgcca tgacccgcga ggccgccggg cccacgacgg gcagcgccgc
     4861 gggaactttc gtcccgacca ccgtggccca gtacgccttc tggtcccacg ggaggagata
     4921 ccccgtgaag ccgaaggcga ggatcaggag gaggagcgcg acgccgacga gccagttcag
     4981 ctcgcgcggc ttccggtacg cgccgaagag gaacgtccgc gcgagatgga gcgcggcgag
     5041 gacgaccatc gcggacgcgc cccagtggtg gaggccgcgc acgacggcgc ctgcggggag
     5101 cttcgtctgg atcgccttca cgctctccca cgcgtggtcc ggcgtcggcg cgtacgagag
     5161 cgcgagcgcg aggcccgtga cgacctggac gacgagcgcg aagagcgcgg ccgagccgag
     5221 cgtgaacagc cacgcccggc ggaccgacac gggttcgtcg gcgaaggcgc gccagagcga
     5281 cgacgctccc gcgcgcgact cgaggaactc gagaagccgc ttcatgcttc gaacgccgcc
     5341 ggatcgaccg tgacgcgtcc gcccgcgacc ttcagcgccg ccttcgggag cggccgcggc
     5401 ggcggccccg cgaggacgcg cccgtcggcg ccgtagacgc cgccgtggca cgggcagagg
     5461 aacgccttct tctcggggtt ccacgcgaca ccgcacccga ggtgcgtgca cgtcgtcgac
     5521 acggccgcga gacccgaagg ggaggacgcg tcccgcacga ccgcgaggcg ctccttgacc
     5581 cggcgcgtca tgtagccgtc ctcgaccgtg cggaggacga cgacctctgc cgcgcccgcc
     5641 gcgccggcct tcacggcctc gaagtccttc gcggcgccga gatcgagcgc cgcgcccccg
     5701 gcggaagcgc ggcgcacgag cggcgacagt gcgaaggaaa gcgcggcgcc ggcgaccgac
     5761 gcgaacgcgg cggcgcccgc ggcgagagcg gtgaaggtga acgcgcgcct cgacggcatc
     5821 gcgggaagga tattccggcc taggtccgtc cgggaccgta aatcagcgcc gcgcaaaccg
     5881 tgccccagat cacaacggtc gcgagcagtc cggcgtccgt gaagaggtcc ttcgtcgcgt
     5941 cctcgccggc tccgcgcgcc tcgagaagac gccagcggaa gaggccgaag aggacgatcg
     6001 gcaccgtgac gaggaggcgg tccgtccgga accacgcgat cgtctcgggg gagacggtgt
     6061 agagcgcgta gcagacgatg gtcgttccga gcgtgatgtt ctcgaacgcc tcgaggaggc
     6121 cgaccggcac ctccttgagc gacgggcgtg cggccgctcc acgcgcggcg agctcgctgc
     6181 gccgcttgga gagcgcgaga aagagcgcga ggaagaacgt gcagaggagg agccagtgcg
     6241 acggcttcac ttgcgccgcc gccgagcccg cgaggacccg caggacgtat ccggacgcga
     6301 cgatgaagac gtcgaggagc gcgaccttct tcaggccgag cgagtagagg accgtgacgc
     6361 ccgcgtacac gagcgtcgcc gggagggccg caggcgccgc gaagaggacg gcgaggagcg
     6421 cgagcggtcc gaggacggcc gccaggacga gggccgtccc gggggggagc gcgcccgacg
     6481 cgaccggccg gtccttcttc acgggatgct ggcggtcggc ctccgcgtcg cgcacgtcgt
     6541 tcaggaggta gagcgccgag gcgagcgcgc agaaggccac ggcgacggcg aacgtgcgtc
     6601 ccgcgagctc cccgcggtcc gcgacctttc cgaagaggac cggcgcgagg acgaagaggt
     6661 tcttgaccca ctgggcgggc cgaagcgcgc ggagagtcgg cgtggcggac aggaatccag
     6721 tttacgcgac cttggtattc tggccgagcc ccgatgcccg cgccggacgc gccccttcgt
     6781 ctggaatcct gcgcctgtcc gctctgcggg gaggcgcctc ccgcgcgcgc gcgcgtttcc
     6841 ttcccgccgt accgggtcgt ggagtgcccg cgctgcacgc tgcgctacct ctcgccgcgc
     6901 gtcgcgcgag aggatctcgg gcgcctctac gagggcggct actgggaggg cggcgggacg
     6961 gagggcggct acgcgtccta cgcggagatg gaggcgctcc tcgcgaagac gttcgcccgg
     7021 cgcctccgct ggctcgggcc gcctcctccc ggagggcggc tcctcgacgt cggatgcggg
     7081 cccggcgcgg gcctcgacgc cgcccgtgcc gccgggtggg aggcctgggg cctcgacctc
     7141 tcctcggagg ccgttgcgcg agcttcggca cggcacccgg ggcgcgtcgc acaaggaacg
     7201 ctcgaagact ctccgtttcc cgccgcctcc ttcgacgcga tcacggcgtt cgacctcgtc
     7261 gagcacgtct acgagccccg gcgtttcgca acggcgctcg cggcggccct gaagcccggc
     7321 ggcagcgtcc tgatcgccac cccgaacgtc gagagcctcc tcgcgcgcgc gaccggccgc
     7381 cggtgggtgt cctataagat tcccgaacac gtgacgtttt tctcgaggag aacgctcgcc
     7441 gacgccctcg cgccgccgct gacgctcgtg cgcgcggcgc cctgcgggca gcacgtctcc
     7501 cttccgttcc tcctcgagcg cgtcggcgcg gcgctgccgg caggcggcgg gctcctccgc
     7561 acggctgcgc gcgggaggac gctctcgggc ctgaccctct acgcgaactc gggctccatg
     7621 ctcgtccgcg ccgtccgcac gacgtgaacg ccgtggacgt ctctgtcgtt ctgcccgtct
     7681 acaacggcgc ggccttcgtg gcggagagcg tctcgggcct cgcggccttc ctcgaacgcc
     7741 aggcgttctc ctgggagatc gtcgtcgtgg acgacggctc gcgcgacgcg acggcccacg
     7801 aggtcccgga tgggaccggg atccatctcc tgcgtctcgc cgagaaccga ggaaagttcg
     7861 gcgcgctgaa ggccggcatg gccgcggcga cgggacgctg ccgcgtcttc acggacgccg
     7921 acgtgccgta cgacctcgag gcgctgccgt acgtggcctc gctcgtgaac gaccgcggct
     7981 tccacgtcgt cgtcggggac cgctcgctga cggactcgat ctacggctcg cgcctcccgc
     8041 gctcccgcgc cgcggcgagc cgcgcgttct cgctcctcgt gcggctcctc gtcacgggcg
     8101 gcctcttcga cacgcagtgc ggcctcaagg gcttccgcgg cgacgtcgcg gacgcgctct
     8161 tcccgctgct caccgtggag ggtttcgcgg gcgacgtcga ggtcctctac gtggcgctga
     8221 agtacaacct ttcgatccgg cggatccccg tgcggctcag gcggcacgaa cggtccacga
     8281 tccgcttcgg cgcgcacgcg cccgcgatgc tccgcgacgc gctcctcctg cggggcaagt
     8341 gggaccgcgg gctctacgcg tcggcggcgc tcgcgaggat cgcggcgcag gactactggg
     8401 tcccgccggc gccggagacg gccgcgtgac gacgcgcgcc gcggcggcgc tcctcgcggc
     8461 ggcgct
//
LOCUS       DAOMFY010000160         8432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2599_length_8432_cov_6.548406, whole genome shotgun sequence.
ACCESSION   DAOMFY010000160 DAOMFY010000000
VERSION     DAOMFY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2599_length_8432_cov_6.548406"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLB01_12690"
     CDS             <1..643
                     /locus_tag="PLB01_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQQ78204.1"
                     /translation="VRAILHMLTPETTSVGGDADFARYSAIAVGPGWGEGTTARETLA
                     RAVAASVPGIFDADALNLAGPAAFFAGRTAPTVLTPHPGEAGRLLGMTADDVNADREA
                     AAVRIARAARAVVILKGFRPVVADASGRVSTVLAGNPALASGGTGDVLTGVVGALLAR
                     GLSAWDAASAAAWLHGTAGDVLREAKGEESVTASDVAEALPDAFLIAREAAAG"
     gene            640..1065
                     /gene="tsaE"
                     /locus_tag="PLB01_12695"
     CDS             640..1065
                     /gene="tsaE"
                     /locus_tag="PLB01_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQQ78205.1"
                     /translation="MTLVASASEEETRRAGAALAARLEPGDAVLVAGDLGAGKTVFVK
                     GLAEGLGLDPDEVSSPSFALVHEYGPAGKAPVLVHADLYRLPGAGGGLEDLGLEARGG
                     AILAVEWPRPPLTGLDAWRVRIDLAEGGARSITIREPNR"
     gene            complement(1129..1791)
                     /locus_tag="PLB01_12700"
     CDS             complement(1129..1791)
                     /locus_tag="PLB01_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQQ78206.1"
                     /translation="MRFRLLALAGCLVFASVAGTAAAQDRAGTFEISPFGGGYFGGNL
                     YDSGRGHVDVQSDWAYGARLAYNVNRVFGIEFDWTHSKADLEGHGFGNVAGYPPYPSS
                     GKIGQLTQDIFEANGIISWGKGRASGYFGFGGGAAVLKTEFNGAGSTSETRFTGNMSL
                     GFKGFVTPRFGFRIDGRWRYIDTDHTTSHNTYCDYYGYCYSYHTTWYSNGELTGGLIF
                     AF"
     gene            complement(1906..3192)
                     /locus_tag="PLB01_12705"
     CDS             complement(1906..3192)
                     /locus_tag="PLB01_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HQQ78207.1"
                     /translation="MIAPAAEAAPRPDVRNWRRRLTVFLAILGPGFITANVDNDAGGI
                     ATYSIAGAHFGYSLLWTLLPITVALIVVQEMSARLGAITGKGLGELIRENYGLRTTFY
                     LMVALLVTDIGNTVAEFAGWAAALEIFGIPRWVSVPIGAFVVWWLVVKGNYKTVEKVF
                     LIACTVYLTYVASALFAKPVWTDVLKQTFEPKIESSAAWILMIIGIVGTTIAPWMQFY
                     LQSSIVEKRIGPEEYRLSRWDVIVGCVVAAVVAFFIVVACGATLYPKGIRIEGAEQAA
                     LALRPLAGKWAGTLFAFGLANASLFAASILPLATAYSICEALGFEAGVDKSWSEAPQF
                     NTLYTSMIVIGAGIILIPGVPLFPVLFLSQVLNGLLLPFVLFFVVLLVNRADLMGTYV
                     NGRAWNWIAWGTVGVMVLLTLVLVVATIWPGLFPAS"
     gene            complement(3189..4496)
                     /locus_tag="PLB01_12710"
     CDS             complement(3189..4496)
                     /locus_tag="PLB01_12710"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQQ78208.1"
                     /translation="MPLPAPPPTRSTAVPAHEGFFVSEFLGEIVRDVARQPVGRIRDL
                     AVSTGVRSPFPKITGLLLRDRFDTFVLPWEDVAIFQRGTVRTRLMKDDLLHRPASEDE
                     IFLARHLLDKQIVDINGVKVVRVNDLKLDIVSGDLVLTAADVGVRGLVRRVFGAGITP
                     RGGRLSDRSLPSRLIPWDSIQPLHPQLDRLETTLPQERLQRLHPADIADILSQVSGTE
                     RTGIFRKLDVETAAETLHELEPEMQAEILEDLPEEHASEILEQMPADEAADVLGEIEG
                     EHARDLVQLMEKEAADEVTELLVHDENTAGGLMGTDFVTFPPHLTVRETLLRLRPIAR
                     DAEVLFYFYVVDEREILLGALSVRELLAAEDDVTLERVMTTPVRSVTPDVKADDVAET
                     MSKYDLLALPVTDPEGVLKGIITIDDVVDRLHDLPKRHRRRLR"
     gene            4557..5621
                     /locus_tag="PLB01_12715"
     CDS             4557..5621
                     /locus_tag="PLB01_12715"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HQQ78209.1"
                     /translation="MAEPGARGVPRSEPVVAVTRGGRVESVHRGSVAVVSEDGTLLAS
                     LGDPRQAVWLRSSAKPFQLAPFLAAGGEERFALSTEEIALAAASHSGEPAHTRVAAAM
                     LAKGGFAESDLHCGAHPPMHEPSARAVWAAGRSPTPLHNNCSGKHAAMLLACRLMDLD
                     PAGYWRKGHPLQRRILASVSRMTGVPAARIARAVDGCSVPVFRVPLASLACAYARLVV
                     GGPRRESLADASARRRIAGAMAAAPEMVAGTGRFTTDLMREFGGTLVAKEGAEGVYAV
                     GVPAPLARRGGKAVGIALKISDGAERGRDAVTVEVLGALGIARGARLARLRRLAFKPV
                     VNVRGDVVGDLRTLFRLGSA"
     gene            5623..6087
                     /locus_tag="PLB01_12720"
     CDS             5623..6087
                     /locus_tag="PLB01_12720"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQQ78210.1"
                     /translation="MRVVRIGKKTEGLLLVVYTAVLLAVTLLVKGGSGVKTNLAPFED
                     IARIVVRAGHGGVLSNAFVYAVVGIVGNLAMFALWAFLLWNFLDGPGRRPARNHADVI
                     LVGVLFSLGIESVQLFIPTRAADINDVFWNVLGTVAGSAFAHLNRNVRFEWE"
     gene            6119..6205
                     /locus_tag="PLB01_12725"
     tRNA            6119..6205
                     /locus_tag="PLB01_12725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6152..6154,aa:Leu,seq:caa)
     gene            complement(6299..6697)
                     /locus_tag="PLB01_12730"
     CDS             complement(6299..6697)
                     /locus_tag="PLB01_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78211.1"
                     /translation="MSLARAVFGPLDRLADRLHPYRWHFVLVALSATAGFLGLGWAVQ
                     EYWPEHTSRVLRYAFPIVVHLAAWGWGARFLVAWYGTRPRGPRFLRRVPQEILDPFAP
                     VKRVWHLFVLLAFLLVPVLVWFAWFRVYYP"
     gene            complement(6707..7633)
                     /locus_tag="PLB01_12735"
     CDS             complement(6707..7633)
                     /locus_tag="PLB01_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78212.1"
                     /translation="MTRIPPFRTAIAPAFAALALSGCAREEPKRKEPDPLTAAINALP
                     EKPGPTLEEIAAERARSADLQRTLDDVQSALEELRVKELKAIRTSIAVAQEGQASAGS
                     RETLKGQIDDIRRSVRANLEKLEALERANHASEERVLGLERLVRELHRQLEEKEETIA
                     ALEERTRELTQTAEGLRGTVKEKEHAVEQVRALLADREAQLAAAYVLIAPRGMLQKVG
                     YVERKGSVLGFGGAWQRTGRVDESLYRRIDIRKVTRFDVGAAADQAIVLSDHPADSYT
                     LAAVSPHESQLTVKDPARFWKMSRQLVVVLPD"
     gene            7781..8302
                     /locus_tag="PLB01_12740"
     CDS             7781..8302
                     /locus_tag="PLB01_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HQQ78213.1"
                     /translation="MAFKIGDKLVYPNHGVGVVETIQESLLEGSPAPCYQLRLLGNNS
                     RVMVPVGNSDRIGLRPLTRRKDVGGVFRVLENGILPPTGDWKGRYKQNLDKMRSGALT
                     DIADVLKNLSWVQKQKALSFREKKMYERARYLIVSEIAQINGLPEGEVATEVEKALDR
                     AMVKRRMGTGRDH"
BASE COUNT         1318 a         2813 c         3031 g         1270 t
ORIGIN      
        1 cgtgcgtgcg attctccaca tgctgacgcc ggagacgacg tcggtcggag gagacgccga
       61 cttcgcgcgg tactcggcca tcgccgtcgg gccggggtgg ggcgagggaa cgacggcgcg
      121 cgagacgctc gcgcgcgccg tcgccgcctc cgtgccgggc atcttcgacg cggacgcgct
      181 gaacctggcc ggcccggcgg cgttcttcgc cggaagaacg gcgccgacgg tcctgacgcc
      241 tcatcccggc gaggccggcc ggctgctcgg aatgacggct gacgacgtga acgccgaccg
      301 cgaggccgcg gccgtgcgga tcgcccgcgc ggcacgggcg gtcgtgatcc tgaagggctt
      361 ccgtcccgtc gtcgcggacg cctccgggcg ggtctccacc gtcctcgcgg gaaaccccgc
      421 gctggcctcg ggaggtacgg gtgacgtcct gaccggcgtc gtcggcgctc tgctcgcacg
      481 cgggctctcg gcgtgggacg ccgcgagcgc cgccgcctgg ctgcacggga cggcgggaga
      541 cgtcctgcgc gaggccaagg gggaggagtc cgtcacggcg tccgacgtgg cggaggcctt
      601 gccggacgcg ttcctcatcg cgcgcgaggc cgccgcggga tgaccctcgt ggcgagcgcg
      661 agcgaggagg agacgcgccg ggcgggcgcc gccctcgcgg cgcggctcga accgggcgac
      721 gcggtgctgg tcgcggggga cctcggggcg ggaaagaccg tcttcgtgaa gggcctggcc
      781 gagggtctcg gactcgaccc cgacgaggtc tcctcgccga gcttcgcgct cgtgcacgag
      841 tacgggcccg ccgggaaggc cccggtcctc gtgcacgccg acctctaccg cctgccgggc
      901 gcgggaggag ggctggagga cctcgggctc gaggcgcgcg gcggcgcgat cctcgcggtc
      961 gagtggccgc gcccgccgct cacgggcctc gacgcctggc gcgtgaggat cgacctcgcg
     1021 gagggcggcg cgcgctcgat cacgatccga gagccgaacc gatagcgcag gcggcggagc
     1081 cgccgagcgg aaggtgcggg aacccgcctg acgggttccc gcttcagatc agaaggcgaa
     1141 gatcagtccg cccgtgagtt cgccgttcga gtaccacgtc gtgtggtacg agtagcagta
     1201 gccgtagtag tcgcagtacg tgttgtggct ggtcgtgtgg tccgtgtcga tgtaccgcca
     1261 gcggccgtcg atgcggaaac cgaagcgcgg cgtgacgaag cccttgaaac cgagagacat
     1321 gttgcccgtg aaacgtgtct ccgacgtgga gccggctccg ttgaactcgg tcttgaggac
     1381 ggccgcgccg ccgccgaagc cgaaataccc ggaagcccgg cccttgcccc acgagattat
     1441 tccgttcgcc tcgaagatgt cctgggtgag ctgtccgatc tttccggacg aagggtaggg
     1501 cggatatccc gcgacgttcc cgaaaccgtg gccctcgagg tcggccttcg aatgggtcca
     1561 gtcgaactcg atgccgaaga cccggttgac gttgtaggcg aggcgggccc cgtaggccca
     1621 gtcgctctgg acgtccacgt ggccgcggcc ggagtcgtag aggttgccgc cgaagtagcc
     1681 gccgccgaag ggcgagatct cgaaggtgcc ggctcggtcc tgtgcggcgg ccgtgcccgc
     1741 gaccgaggcg aacacgagac acccggcgag ggcgagaaga cggaatcgca tggtcaaacc
     1801 tcctgcaaaa ccgacgtgtt gcgggcggcc ggcgtctccg gagcgtccgc cgggggagac
     1861 gaatgcagag ccatggaact cgtaagcaac ccccgttccg gcatgctacg acgccgggaa
     1921 caggcctggc cagatcgtcg cgacgacgag cacgagcgtc agcaggacca tgacgcccac
     1981 cgtcccccac gcgatccagt tccaggcgcg gccgttgacg tacgtcccca tcaggtcggc
     2041 ccggttcacc agaagaacca cgaagaagag gacgaacgga aggagcaggc cgttcaggac
     2101 ctgggacagg aagaggacgg gaaagagcgg gacgccgggg atcaggatga tgcccgcccc
     2161 gatcacgatc atggacgtgt agagcgtgtt gaactggggc gcctcggacc acgacttgtc
     2221 gacgccggcc tcgaagccga gggcctcgca gatcgagtac gccgtcgcga ggggaaggat
     2281 ggacgccgcg aagagcgagg cgttcgcgag gccgaaggcg aacagggtgc cggcccactt
     2341 cccggcgagc ggcctgagcg cgagcgcggc ctgctcggcg ccctcgatgc ggatcccctt
     2401 cggatagagc gttgcgccgc aggcgacgac gatgaagaac gcgacgacgg ccgcgacgac
     2461 gcagcccacg atgacgtccc agcgcgagag ccggtactcc tcgggcccga tgcgcttctc
     2521 gacgatcgag gactgcaggt agaactgcat ccacggcgcg atggtcgtcc cgacgatgcc
     2581 gatgatcatg aggatccacg cggcgctcga ctcgatcttc ggttcgaatg tctgcttgag
     2641 gacgtcggtc cacacgggct tggcgaagag cgcggaggcg acgtacgtga ggtacaccgt
     2701 gcacgcgatc aggaagacct tctcgaccgt cttgtaattg cccttcacga cgagccacca
     2761 gacgacgaac gccccgatcg ggacggagac ccatcgcggg attccgaaga tctcgagcgc
     2821 cgcggcccac cccgcgaact cggcgaccgt gttcccgatg tccgtgacga ggagcgcgac
     2881 catcaggtag aacgtcgtgc gcaggccgta gttctcgcgg atgagctcgc cgaggccctt
     2941 gcccgtgatc gcgccgagcc gcgcggacat ctcctggacg acgatgaggg cgaccgtgat
     3001 cgggaggagc gtccagagga gcgagtagcc gaagtgcgcg cccgcgatcg agtacgtcgc
     3061 gatgccgccg gcgtcgttgt cgacgttcgc ggtgatgaac ccggggccga ggatcgcgag
     3121 gaagacggtg aggcggcggc gccagttccg gacgtccggg cgcggcgccg cttccgcggc
     3181 cggagcgatc accgcagcct ccggcggtgg cgcttcggca ggtcgtggag acggtccacg
     3241 acgtcgtcga tcgtgatgat ccccttgagg acgccctcgg ggtccgtcac ggggagggcg
     3301 agaaggtcgt acttcgacat cgtctcggcc acgtcgtcgg ccttgacgtc gggtgtcacg
     3361 ctcctcacgg gcgtcgtcat gactctctcg agcgtcacgt cgtcctccgc ggccaggagc
     3421 tcgcggaccg aaagcgcccc gaggaggatc tcgcgctcgt cgacgacgta gaagtagaag
     3481 aggacctcgg cgtcccgcgc gatggggcgg aggcggagga gcgtctcgcg cacggtgagg
     3541 tgcggcggga acgtcacgaa gtccgtcccc atgaggccgc ccgccgtgtt ctcgtcgtgg
     3601 acgaggagct ccgtgacctc gtcggcggct tccttctcca tgagctggac gaggtcgcgc
     3661 gcgtgctcgc cctcgatctc gccgaggacg tcggccgcct cgtccgcggg catctgctcg
     3721 aggatctccg aggcgtgctc ctcgggcagg tcctcgagga tctcggcctg catctcgggc
     3781 tcgagctcgt gcagcgtctc ggcagcggtc tcgacgtcga gcttgcggaa gatcccggtg
     3841 cgttcggtcc ccgagacctg gctcaggatg tcggcgatgt ccgccgggtg gaggcgctgg
     3901 aggcgctcct gggggagcgt cgtctcgagt cggtcgagct gggggtggag cggctggatc
     3961 gagtcccacg ggatcaggcg cgaggggaga gagcggtccg agagccgacc gccgcgcggc
     4021 gtgatccccg ccccgaagac gcgccgcacg aggccgcgca cgccgacgtc ggccgccgtg
     4081 aggacgaggt cgccggagac gatgtcgagc ttgaggtcgt tgacgcggac gactttcacg
     4141 ccgttgatgt cgacgatctg cttgtcgagg aggtggcgcg cgaggaagat ctcgtcctcg
     4201 ctggccggcc ggtggaggag gtcgtccttc atgagccgcg tgcgcacggt cccgcgctgg
     4261 aagatcgcga cgtcctccca cgggaggacg aacgtgtcga aacggtcgcg gaggaggagg
     4321 cccgtgatct tcgggaacgg cgaccgcacg ccggtcgaga ccgcgaggtc gcggatcctg
     4381 ccgacgggct ggcgcgccac gtcgcgcacg atctcgccga ggaactcgga cacgaagaac
     4441 ccttcgtgcg ccggaacggc ggtcgagcgg gtcggaggcg gcgcgggaag cggcacgaca
     4501 cattgtcttg gcggatgcgc gtcgcgtcaa cgaattcggg taatctcgcg cgccaaatgg
     4561 cggaacccgg agcgcgcggc gtcccccgga gcgagccggt cgtcgccgtg acgcgcggcg
     4621 gacgcgtcga gtccgtgcac cgcggctccg tcgcggtcgt ttcggaggat ggaacgctcc
     4681 tcgcgtctct cggcgacccg cgccaggccg tgtggctgcg ctcctcggcc aagccgtttc
     4741 agctcgcccc gttcctcgcc gcgggcgggg aggaacggtt cgccctttcg accgaggaga
     4801 tcgcgctcgc ggccgcctcg cattccgggg agcctgcgca cacgcgggtc gccgccgcga
     4861 tgctcgcgaa gggaggcttc gcggagagcg atctgcactg cggcgcgcat ccgcccatgc
     4921 acgagccgtc ggcccgcgcc gtttgggccg cgggccggag cccgacgccc ctccacaaca
     4981 actgctcggg caagcacgcg gccatgctcc tcgcctgccg cctgatggac ctcgatcccg
     5041 cggggtactg gagaaagggg cacccgctcc agaggcggat cctcgcctcg gtgtcccgca
     5101 tgaccggcgt tcccgctgcg cggatcgcac gcgccgtcga cggctgctcc gttccggtct
     5161 tccgggttcc gctcgcctct ctcgcgtgcg cctacgcccg cctcgtcgtc ggcgggccgc
     5221 gccgggagag cctcgcggac gcgtccgcgc gccggaggat cgcgggcgcg atggccgcgg
     5281 cgcccgagat ggtggccggg accggccgtt tcacgacgga cctcatgcgc gaattcggcg
     5341 ggactctcgt ggccaaggaa ggggcggaag gggtctacgc ggtgggcgtt ccggcccctc
     5401 tcgcgcgacg cggcgggaag gcggtcggga tcgccctgaa gatctcggac ggcgccgagc
     5461 gcgggcgcga cgccgtcacc gtcgaggtcc tcggcgcact cgggatcgcg aggggagcgc
     5521 gcctcgcgcg cctccggagg ctcgcgttca agccggtcgt caacgtgcgc ggggacgtcg
     5581 tgggcgacct ccgcaccctg ttccggctcg ggagcgcctg acgtgcgcgt cgtgaggatc
     5641 ggaaagaaga cggaagggct tctcctcgtc gtgtacacgg ccgtgctcct cgccgtgacc
     5701 ctgctcgtga agggcggcag cggcgtgaag acgaacctcg cgccattcga ggacatcgcg
     5761 cgcatcgtcg tgcgtgcggg acacggaggc gtcctgtcga acgcgttcgt ctacgccgtc
     5821 gtgggcatcg tcgggaacct cgccatgttc gcgctctggg ccttcctgct ttggaacttc
     5881 ctcgacgggc ccggccgccg gccggcgcga aaccacgcgg acgtcatcct cgtgggcgtc
     5941 ctcttctcgc tcggcatcga gagcgtccag ctcttcatcc cgacgcgcgc cgcggacatc
     6001 aacgacgtgt tctggaacgt gctcggcacg gtcgcgggca gcgccttcgc gcacctcaac
     6061 cgcaacgtgc gcttcgagtg ggagtgacgg gaccgtagaa tcgggattgc gacggcctgc
     6121 cggagtggcg gaatggcaga cgcaggggac tcaaaatccc ccggcccaca aggccgtgag
     6181 ggttcgaccc cctcctccgg caccaactga gatacacgag atggtggtgt cgccaaggta
     6241 ttcataccta ataaaataag gaagacttta tattcttccg tatttcataa gggattacct
     6301 acggatagta aacgcggaac cacgcgaacc agacgaggac cgggacgagg aggaaggcca
     6361 ggagaacgaa gaggtgccag acgcgcttca cgggcgcgaa aggatcgaga atctcctgcg
     6421 ggacgcggcg caggaatcgc ggtccgcgcg ggcgcgtccc gtaccacgcg acgaggaagc
     6481 gagcgcccca tccccaggcc gcgaggtgca ccacgatcgg aaacgcgtac cgcaggacgc
     6541 gcgacgtatg ctcgggccag tactcctgaa ccgcccagcc gagcccgagg aagccggcgg
     6601 tggcagacag cgcgacgagg acgaagtgcc agcgatacgg atgaagcctg tccgcgaggc
     6661 gatcgagggg tccgaacacg gcgcgtgcga gtgacacggt gggccttcag tcgggcagga
     6721 cgacgacgag ctggcggctc atcttccaga agcgcgcggg gtccttcacg gtgagctggg
     6781 actcgtgcgg gctcacggcc gcgagcgtgt agctgtcggc ggggtggtcg gagaggacga
     6841 tcgcctggtc ggccgccgcc ccgacgtcga accgggtcac ctttcggatg tcgatgcggc
     6901 gatagagcga ctcgtcgacg cgccccgtcc tctgccaggc gccgccgaaa ccgaggacgg
     6961 accccttcct ctcgacgtat ccgaccttct ggagcatccc cctcggcgcg atcaggacgt
     7021 acgccgcggc gagctgcgcc tcgcggtccg cgaggagcgc ccgcacctgc tcgacggcgt
     7081 gctccttttc tttcacggtt ccacgcagcc cctccgcggt ctgagtgagc tcgcgcgtcc
     7141 gctcctcgag ggcggcgatc gtctcctctt tctcctcgag ctgccggtgc agctcgcgaa
     7201 cgaggcgttc caggccgagg accctttcct ccgaagcgtg gttcgcccgc tcgagggctt
     7261 cgagcttctc gaggttggcc ctcacggacc gtcggatgtc gtcgatctgg cccttcaggg
     7321 tttcgcggct gcccgccgag gcctgcccct cctgcgcgac cgcgatcgac gtccggatcg
     7381 ccttgagctc cttgacccgc agctcctcga gggcgctctg gacgtcgtcc agcgtgcgct
     7441 gcaggtcggc gctgcgggcg cgctccgcgg cgatttcctc gagcgtcggg ccgggcttct
     7501 ccggaagcgc gttgatcgcc gccgtgagcg ggtcgggttc cttccttttc ggctcttccc
     7561 gcgcgcaacc cgacagggcg agggccgcga acgcgggcgc aattgccgtt cgaaacgggg
     7621 gaattctcgt catttcgcgt ccagggaatc tgcgactcaa tcgtactctt tcgcctaaat
     7681 cgttcaacac gcgccacaag cgcgaatgcc tgtcatactc gttgacctgt ccggtggggg
     7741 agagtagtct ctctttcccc tttccaagga ggagttggcc gtggcgttca agatcggcga
     7801 taagctcgtc tacccgaacc acggtgtcgg cgtggtggag accatccagg aatcgctcct
     7861 cgagggctcg cctgcgcctt gctaccagct ccgcctgctg ggcaacaatt cgcgcgtcat
     7921 ggttcccgtg ggcaactcgg accggatcgg cctgcgcccg ctcacgcgcc gcaaggacgt
     7981 cggcggagtc ttccgcgtcc tcgagaacgg catcctgccg ccgacgggcg actggaaggg
     8041 ccgctacaag cagaacctcg acaagatgcg ctcgggcgcc ctcacggaca tcgccgacgt
     8101 gctcaagaac ctgagctggg tccagaagca gaaggcgctc tcgttccgcg agaagaagat
     8161 gtacgagcgc gcgcgctacc tgatcgtctc ggagatcgcc cagatcaacg ggctccccga
     8221 gggcgaggtc gcgaccgagg tcgaaaaggc gctggaccgg gccatggtca agcgccgcat
     8281 ggggacgggc cgagaccact gaagtgccca gggagaaccc gagcgcgggt tctccctgac
     8341 cctctccgcc gcccccggcg ccccctcccg cccggggtcc tcgtagtgcg agtgcattcg
     8401 cgatgcgggg ggcgggaatt ccgtatagcc gg
//
LOCUS       DAOMFY010000161         8338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2638_length_8338_cov_7.666667, whole genome shotgun sequence.
ACCESSION   DAOMFY010000161 DAOMFY010000000
VERSION     DAOMFY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_8338_cov_7.666667"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..2525)
                     /locus_tag="PLB01_12745"
     CDS             complement(270..2525)
                     /locus_tag="PLB01_12745"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HQQ78214.1"
                     /translation="MSFTKQDALDYHSTGRKGKLEVVPSKPCATARDLSLAYTPGVAE
                     PCLEIARDPELVYEYTGKGNLVAVISNGTAVLGLGDIGAAAGKPVMEGKGVLFKRFAD
                     VDVFDIEVAEHDPDKLIECVARLEPTFGGINLEDIKAPECFYIEEQLKKRMGIPVFHD
                     DQHGTAIISAAAFLNALEIAGKKIEDVKVVFAGAGAAAVACANLYITYGVKLENILMV
                     DRTGVIWKGRTENMNEYKERFARDTKKRTVAEALEGADAFIGVSAAKLINGDMIKKMA
                     KDPIVFAMANPVPEILPEEAKAARPDVIMATGRSDYPNQVNNVLGFPFIFRGALDCRA
                     TQINEAMKLAASKALANLAKEDVPDSVARAYGVDGFRFGREYLIPKPFDWRVLLHVPP
                     AVAQAAADTGVAKKPIKDMEAYKRKLEGMLSRSRSFMQVIHGKAKGDPKRVVLAEGDH
                     PKIQRAAKMLAEEGLAKPILLSRGKKAEAALKAMDVKVPIEVIDPETHPNYAKYVKAF
                     HEKRRRSGVTLEDAEHTLRSRNYFAGMMVDQGDADVTLSGLTASYPDTIKPALQTIGV
                     AAGVKKVSGLYVLILQDRTFFFADTTVNIDPTAEDLAEIALLSADTAKLFGILPRVAM
                     IAFSNFGSTVHPETNKVREAVKLVKQRRPDLECDGEMQADTAVLPEFLKEHYPWTDLK
                     GPANVLVFPELQSANAAYKLVWRLAKADAIGPILMGMSKPAHVLQRGVEVADIVNMAA
                     FGVVDAQARKK"
     gene            2646..4808
                     /locus_tag="PLB01_12750"
     CDS             2646..4808
                     /locus_tag="PLB01_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HQQ78215.1"
                     /translation="MKRAFFGAVLTTLLAVVAPAASQPFDGFATFTRNGGMYFQAPSA
                     GSGQPASAGTWEAWVKLTATPSASDCGSLIGKGYVTSYWIGICSEGTPPTLRSYTRGT
                     ASAFTSGQIPVGTWTHIAVTTDGATRRHYLNGVLAGSQADGGAPTPSTNPLRIGSDAN
                     WNFQSPVLVDEVRIWNVARTANEIKSTMWAPVASSATGLLDVWHFDGDATDGTGAANL
                     TGVGATAFGTTNTGTRTLFVPVVLKNQYSSEITFTNRGTTTATVTMQYTASSGGGSGT
                     AAAFTLAPGRQRVEADAIAFLGLQGLPIDATGTRLGTLRVTFTGLSSNDAAAVTVRTA
                     TDTTNPVGRAGLSYAGVPVERTFTGTVYLPSLAFSTSGVRTNLALQNAGAPADGNVTL
                     RVTLYNTTGGTFGILPDVTLPPGGFRQFSSAEMGLPENYSGGAKVEVVGGTARFYAYA
                     VQNDQVNSDGSFVLPVDPATATRSTVTIPSVVEAGTYRTELTIYNVGDVTRNIVASLL
                     CPSCPIPAGIGFAVPAKGEVYFQDFIAFCRASSFGGSLANPLGVAVVTLNELDASMNV
                     QDLVVVARTSSAVAGQGKFGVAYPSVRAAEGAPYATWIYAARQDATNRSNLAFVNLGD
                     LDAAPITLHLDVYDGTTGALAATVDDPRLAGIAAGGFVQINGFLAAFAPGISSGYVKV
                     TRTGGVNRFTSYLVVNDGGNPGERTGDGAYVAMDVPYR"
     gene            complement(4812..5633)
                     /locus_tag="PLB01_12755"
     CDS             complement(4812..5633)
                     /locus_tag="PLB01_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ78216.1"
                     /translation="MALDETRLTEHVSVFTHGGETLAESYGSNATVFFGKGAVVVVDP
                     FVAPRQAAALGARIADRTPDPVTHVILTHHHTDHALGASFFAALGADVIAHEAAARRM
                     AAEHAGLIAARRRDPSLAGLFDGAEPHAPRRLVRDSLAFEAGGIRFDVFHPGHAHTPG
                     DLCVYVPALGVLVSGDLVSTAYHPNLEDADVARWRAALVGLRGIPFWSLVPGHGPSGG
                     REAVEDQIAYFDTAERTVREAAAFGLDPVAALKAAFPERLLAVVLPDTVRRLAAA"
     gene            complement(5650..7431)
                     /locus_tag="PLB01_12760"
     CDS             complement(5650..7431)
                     /locus_tag="PLB01_12760"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQQ78217.1"
                     /translation="MRWSQSFLVTLREVPGDAEVVSHQLLYRAGFIQKLAAGIYSYTP
                     FGFRSLRKMIEIVREEMDATGAQEVDLPILQPKELWVESGRWDRYQAEGILFHLKDRK
                     DGEFALAPTAEEAVTDMVRRSVTSWRQLPFNLYQIRTKFRDEIRPRFGLLRGREFLMK
                     DAYSFDLDQKGLDAHYEKMDAAYRKIFDRCGLEFAVVQADSGAIGGSASEEFMVVADT
                     GEDALVFAEAGDYGANIEKAVTAPLPEPWAGEDAKPFQKAGATTPGITTTDGQAKHLA
                     TEPARIVNTMLYEAVGPDGKARLVAVLIRGDLAINEVKLPKALGALHVKLASEEILRK
                     VTGTRPGFVGPLGLQPFEENPISLFVDRSLEHGVNVWCGANLDDHHHAQVSIKRDVMS
                     DPAFAGVVEVATARDGDPSPAPKGGALRIKRGIEVGHIFKLGTKYSEAMKCEVADQAQ
                     KMVPLAMGCYGLGLGRTIAAAIEQHHDEHGIVWPVPLAPYACVVAALQRDADVLAAAD
                     KLYGDLKAAGVEVFYDDRDERPGVKFKDIDLIGFPVRVVVGGKSLAKGVVEVSTRREK
                     QAQGIALADVVGVVKKTLAAGAKTLAV"
     gene            complement(7465..7752)
                     /locus_tag="PLB01_12765"
     CDS             complement(7465..7752)
                     /locus_tag="PLB01_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQQ78218.1"
                     /translation="MRLDRVIHERLRLGIVSALAGAPSLTFNELKGILGATDGNLSVH
                     ARRLEEAGYVCCTKGYDGRVPKTEFRLTEEGRRALRRYLDHLQALIEATKR"
     gene            complement(7820..>8338)
                     /locus_tag="PLB01_12770"
     CDS             complement(7820..>8338)
                     /locus_tag="PLB01_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78219.1"
                     /translation="MGRAAAFTCVPGKGGVAMGAVGVAAAAWAARASSTREWLAIWTG
                     AAVLAFGIGAFGLLRKAARFDHRPSVSAARRFVLALLPPLVAGAALTAALASSAAVAF
                     LPGIWLLLYGCAVVAAGLLSVPVVPLFGGALLALGLLALVFPAHGDLLLGSGFGLGHV
                     VCGVIVARRHGG"
BASE COUNT         1232 a         2882 c         2931 g         1293 t
ORIGIN      
        1 caggtcccag gagaggcgcg cgttgccgaa ggcgcgcgcg gccaccggca gcgagagctc
       61 cttgtcgtcc cgccagttgc gggcgagggt cacgctggcc ccgtagcgca ggcggccgcg
      121 cgcccccccg cggcgccatc aggcgccgcc agcggaggaa tgcgtcgagg gccgggatcg
      181 ccgagtacgc ggcgaggagc ccgcagccgg tcgtgaggat gtccacgcgg cccgcatcgt
      241 aacggacggg cgggccgcgt ggccgggagc tacttcttgc gcgcctgggc gtccacgacg
      301 ccgaaggccg ccatgttcac gatgtcggcg acctcgacgc cccgctggag gacgtgcgcg
      361 ggcttgctca tccccatgag gatcggcccg atggcgtcgg ccttcgcgag gcgccagacc
      421 agcttgtagg ccgcgttcgc gctctggagc tcggggaaga cgaggacgtt tgccgggccc
      481 ttgaggtccg tccaggggta gtgctccttg aggaactccg gaaggacggc cgtgtccgcc
      541 tgcatctcgc cgtcgcactc gagatccggg cggcgctgct tgacgagctt gaccgcctcg
      601 cgcaccttgt tcgtctccgg gtgcaccgtc gagccgaagt tcgagaacgc gatcatggcg
      661 acgcgcggca ggatcccgaa cagcttcgcc gtgtcggccg agaggagcgc gatctcggcg
      721 aggtcctcgg ccgtgggatc gatgttcacg gtcgtgtccg cgaagaagaa cgtgcgatcc
      781 tggaggatga ggacgtagag gcccgagacc ttcttgacgc ccgccgcgac gccgatcgtc
      841 tggagggccg gcttgatcgt gtccggatac gacgccgtga ggcccgagag agtgacgtcc
      901 gcgtcgccct ggtcgaccat catcccggcg aagtagttcc gcgaccgcag cgtgtgctcg
      961 gcgtcctcga gggtcacgcc gctgcgccgg cgcttttcgt ggaaggcctt gacgtacttc
     1021 gcgtagttcg ggtgcgtctc cgggtcgatg acctcgatcg gcaccttgac gtccatggcc
     1081 ttgagggccg cctcggcctt cttcccgcgc gagaggagga tcggctttgc gagcccctcc
     1141 tcggcgagca tcttggccgc acgctggatc ttcgggtggt cgccctcggc gaggacgacg
     1201 cgcttcggat cgcccttggc cttgccgtgg atgacctgca tgaacgagcg cgagcgcgag
     1261 agcatgccct cgagcttccg cttgtacgcc tccatgtcct tgatcggctt cttggcgacg
     1321 ccggtgtcgg cggcggcctg cgcgacggcc ggcggaacgt gcaggagcac gcgccagtcg
     1381 aagggcttgg ggatcaggta ctcccggccg aacctgaagc cgtccacgcc gtacgcgcgg
     1441 gcgacggagt ccgggacgtc ctccttcgcg aggttcgcga gggccttgga ggcggcgagc
     1501 ttcatcgcct cgttgatctg cgtggcgcgg cagtcgaggg cgccgcggaa gatgaacggg
     1561 aagccgagca cgttgttgac ctggttcggg tagtcggagc ggccggtcgc catgatcacg
     1621 tcgggccgcg cggcctttgc ctcctccggg aggatctcgg gcaccgggtt cgccatggcg
     1681 aagacgatcg ggtccttcgc catcttcttg atcatgtcgc cgttgatgag cttcgcggcc
     1741 gaaacgccga tgaacgcgtc cgctccctcg agggcctcgg cgaccgtgcg cttcttcgtg
     1801 tcgcgcgcga atcgctcctt gtactcgttc atgttctccg tccggccctt ccagatgacg
     1861 ccggtgcggt cgaccatgag gatgttctcg agcttcacgc cgtacgtgat gtagaggttc
     1921 gcgcaggcga cggcggccgc gcccgcgccc gcgaacacga ccttcacgtc ctcgatcttc
     1981 ttgcccgcga tctcgagcgc gttcaggaac gccgcggccg agatgatcgc ggtcccgtgc
     2041 tggtcgtcgt ggaagaccgg gatccccatg cgcttcttga gctgctcctc gatgtagaag
     2101 cactcgggcg ccttgatgtc ctcgaggttg atgcccccga acgtgggctc gaggcgggcg
     2161 acgcactcga tgagcttgtc ggggtcgtgt tcggcgacct cgatgtcgaa cacgtcgacg
     2221 tccgcgaacc tcttgaacag gactcccttg ccttccatga ccggcttgcc ggcggccgcg
     2281 ccgatgtcgc cgagaccgag gacggcggtg ccgttcgaga tgacggccac gagattcccc
     2341 ttgccggtgt actcgtagac gagctcgggg tcccgggcaa tttcgagaca cggctcggcg
     2401 acgccgggcg tgtaggcgag cgaaaggtcg cgggcggttg cgcagggttt ggaggggacg
     2461 acttccagct ttcccttgcg gcccgtcgag tggtagtcga gggcgtcctg tttggtgaag
     2521 ctcacgcgtg tcttcctttc ttcctgaacc gggcgatgaa tcttagtccc ggcattccgc
     2581 gcctcccgat gatgcgggcg catcagggcc ccaaaagggt gagacaatgt ctcaacggag
     2641 gctccatgaa gagagctttt ttcggagcgg tcctgaccac cctgctcgcg gttgtcgcgc
     2701 ctgcggcctc ccagccgttc gacggcttcg ccacgttcac gcgcaacggc ggcatgtact
     2761 tccaggcccc gtccgccggg tccggccagc ccgcgtcggc cgggacctgg gaggcgtggg
     2821 tgaagctgac ggcgacgccg tcggcctccg actgcggaag cctcatcggc aagggctacg
     2881 tcacgtccta ctggatcggg atctgctcgg aggggacgcc gcccactctg cgttcgtaca
     2941 cgcgcggcac cgccagtgcg ttcacgtccg gtcagatccc cgtggggacg tggactcaca
     3001 tcgccgtcac gaccgacggc gcgacgcgcc gacactacct gaacggcgtc ctcgccggct
     3061 cgcaggccga cggcggcgcc ccgacgccca gcacgaaccc gctccggatc gggagcgacg
     3121 cgaactggaa cttccagtcg cccgtcctcg tggacgaggt gcggatctgg aacgtggccc
     3181 ggaccgcgaa cgagatcaag agcacgatgt gggcgcccgt cgcgtcgagt gcgacggggc
     3241 tcctcgacgt ctggcacttc gacggcgacg cgaccgacgg cacgggcgcg gcgaacctga
     3301 ccggcgtcgg cgcgacggcc ttcggcacga cgaacacggg cacgcggact ctcttcgtcc
     3361 ccgtcgtcct caagaaccag tattcgtccg agatcacgtt cacgaaccgc gggacgacga
     3421 ccgcgaccgt cacgatgcag tacacggcga gttccggagg cggctccggg acggccgcgg
     3481 ccttcacgct cgcccccggc cgccagcgcg tcgaggcgga cgcgatcgcg tttctcggcc
     3541 tccagggcct tccgatcgac gcgacgggta cgcgcctcgg gacgctccgc gtcacgttca
     3601 ccgggctctc ctcgaacgac gcggccgccg tcacggtgcg cacggccacg gacacgacga
     3661 acccggtggg ccgcgccggc ctttcctacg cgggcgtgcc ggtcgagagg acgttcacgg
     3721 gaaccgtcta tctcccctcc ctcgccttca gcacctcggg cgtcaggacg aatctcgcgc
     3781 tccagaatgc cggcgcgccg gcggacggga acgtcacgct ccgggtgacg ctctacaaca
     3841 cgaccggcgg gacgttcggg atcctgcccg acgtcacgct gcctcccggc ggcttccgcc
     3901 agttctcctc cgccgagatg gggttgccgg agaactactc cggcggggcc aaggtcgaag
     3961 tcgtcggcgg gaccgcgcgt ttctacgcgt acgccgtcca gaacgaccag gtcaacagcg
     4021 acggctcctt cgtcctgccc gtcgaccccg ccaccgcgac ccgctcgacg gtcacgatcc
     4081 cgtccgtcgt cgaggcaggc acctaccgga cggagctcac gatctacaac gtcggggacg
     4141 tcacgcggaa catcgtcgcg tccctcctct gcccgtcctg cccgattccc gcggggatcg
     4201 gcttcgccgt gccggcgaag ggcgaggtct acttccagga cttcatcgcc ttctgccgcg
     4261 cctcctcgtt cggcggctcc ctcgcgaacc ctctcggcgt cgccgtcgtc acgctgaacg
     4321 agctcgacgc gtcgatgaac gtgcaggatc tcgtcgtcgt cgcgcgcacg tcgtccgcgg
     4381 ttgcggggca gggcaagttc ggcgtcgcgt acccgtccgt gcgcgccgcg gaaggcgctc
     4441 cctacgcgac gtggatctac gcggcgcggc aggacgcgac gaaccggtcg aacctcgcgt
     4501 tcgtgaacct cggcgacctc gacgccgcgc cgatcacgct ccatctcgac gtctacgacg
     4561 ggacgacggg cgccctcgcg gccaccgtgg acgacccgcg cctcgcggga atcgcggccg
     4621 gcggattcgt ccagatcaac gggttcctcg cggcgtttgc gccggggatc tcgagcggct
     4681 acgtgaaggt cacgcgcacg gggggggtga acagattcac gtcgtacctc gtcgtgaacg
     4741 acggcgggaa cccgggcgaa cgcaccggcg acggggcgta cgtcgcgatg gacgtgccct
     4801 accgctgacg ctcaggccgc cgcgaggcga cggacggtgt ccgggaggac gacggcgagc
     4861 agccgctcgg ggaaggcggc cttgagggcg gcgacggggt ccagcccgaa cgccgccgcc
     4921 tccctgacgg tccgctcggc cgtgtcgaag tacgcgatct ggtcctcgac cgcttcgcgc
     4981 ccgccggagg gtccgtggcc cgggacgagc gaccagaacg gaatcccgcg gagcccgacg
     5041 agagccgcgc gccagcgggc gacgtccgca tcctccaggt tcgggtggta cgcggtcgac
     5101 acgaggtcgc ccgacacgag gactccgagc gccggcacgt acacgcagag gtcgccgggc
     5161 gtatgggcgt ggcccgggtg gaagacgtcg aaccggattc ccccggcctc gaatgcgagc
     5221 gagtcccgga ccaggcgcct cggcgcgtgc ggttcggcgc cgtcgaagag gccggcgagc
     5281 gacgggtcgc ggcgcctcgc ggcgatcagc ccggcgtgct cggcggccat gcgccgcgcg
     5341 gcggcttcgt gcgcgatcac gtccgcgccg agcgccgcga agaaagacgc cccgagcgcg
     5401 tggtccgtgt ggtggtgcgt gagaatgacg tgcgtgaccg ggtcgggcgt gcggtccgcg
     5461 atccgcgcgc cgagcgccgc cgcctgccgg ggcgcgacga acgggtccac gacgacgacc
     5521 gcgcccttcc cgaagaaaac cgtcgcgttc gagccgtagc tctcggcgag cgtctcgccg
     5581 ccgtgcgtga agaccgaaac gtgctcggta aggcgggttt cgtccagcgc cacggcggtg
     5641 cccgcgctcc tacacggcca gagtcttcgc gcccgcggcg agcgtcttct tcacgacgcc
     5701 gacgacgtcc gcgagggcga tcccctgcgc ctgcttctcg cgccgcgtcg agacctcgac
     5761 gacgcccttc gcgagcgact tcccgccgac gacgacccgc acggggaagc cgatgaggtc
     5821 gatgtccttg aacttcacgc ccggccgctc gtcgcggtcg tcgtaaaaca cctcgacgcc
     5881 ggcggccttc aggtccccgt agagcttgtc ggcggcggcg aggacgtcgg cgtcgcgctg
     5941 gagggccgcg acgacgcacg cgtacggcgc gagcgggacg ggccacacga tgccgtgctc
     6001 gtcgtggtgc tgctcgatgg cggccgcgat cgtgcggccg aggccgagcc cgtagcagcc
     6061 catcgcgagc ggcaccatct tctgcgcctg gtcggcgacc tcgcacttca tggcctcgga
     6121 gtacttcgtg ccgagcttga agatgtgccc gacctcgatg ccgcgcttga tgcgcagcgc
     6181 gccgcccttc ggcgccggcg agggatcgcc gtcgcgggcg gtcgcgacct cgacgacgcc
     6241 ggcaaaagcc ggatcggaca tgacgtcacg cttgatggac acctgagcat gatgatggtc
     6301 gtcgaggttt gccccgcacc atacgttgac tccgtgctcc agggaccggt cgacgaagag
     6361 ggagatcgga ttttcttcga atggttgaag accgagcggc ccgacgaatc cggggcgcgt
     6421 ccctgtgacc tttcgaagaa tctcctccga agcgagcttc acgtgaagcg cgccgagcgc
     6481 cttgggaagc ttgacctcgt tgatggcgag atcgccgcgg atgagaaccg cgacgagccg
     6541 tgccttgccg tccggtccaa cggcttcgta gagcatcgtg ttcacgatgc gcgccggttc
     6601 ggtcgcgaga tgtttggcct ggccgtccgt agtcgtaatg cccggggtcg tggcgcccgc
     6661 cttctggaac ggcttcgcgt cctcccccgc ccacggctcg gggagcggcg ctgtcacggc
     6721 cttctcgatg ttcgcgccgt aatcgccggc ttccgcgaac acgagcgcgt cctcgccggt
     6781 gtcggcgacg accatgaact cttccgaagc cgagccgccg atcgcgcccg agtccgcctg
     6841 cacgaccgcg aactcgaggc cgcagcggtc gaagatcttc cggtacgccg cgtccatctt
     6901 ctcgtagtgc gcgtcgaggc ccttctggtc gaggtcgaag gaatacgcgt ccttcatgag
     6961 gaactcgcgg ccgcgcagga ggccgaagcg cggccggatc tcgtcgcgga acttcgtgcg
     7021 gatctggtac aggttgaacg ggagctgccg ccaggacgtc acggagcgcc ggaccatgtc
     7081 cgtcacggcc tcctcggcgg tcggcgcgag cgcgaactcg ccgtccttgc ggtccttgag
     7141 gtggaagagg attccctcgg cctggtagcg gtcccagcgg cccgactcga cccacagctc
     7201 cttcggctgg aggatcggga ggtccacttc ctgggcgccc gtggcgtcca tctcctcgcg
     7261 cacgatctcg atcatcttcc tgagcgagcg aaagccgaac ggcgtgtacg agtagatgcc
     7321 ggcggcgagc ttctggatga agcccgcgcg gtagaggagc tgatgggaga ccacctcggc
     7381 gtcgccgggg acctcccgga gggtcacgag aaacgactgg gaccagcgca taggccgcgg
     7441 agattagcag gatgaggcgc ggcctcagcg cttcgtcgcc tcgatcaggg cttggagatg
     7501 gtcgaggtag cggcgcagcg cgcggcgtcc ctcctcggtg aggcggaatt cggtcttcgg
     7561 cacgcggccg tcgtagccct tcgtgcagca gacgtaaccc gcctcctcga gccggcgcgc
     7621 gtggaccgac aggttgccgt cggtcgctcc gaggattccc ttcagctcgt tgaatgtgag
     7681 ggacggcgca cccgcgagcg cgctcacgat tccgaggcgc aggcgctcgt ggatcacgcg
     7741 gtcgaggcgc atcgcctcgg aggacgccgg cgccgccttc ctcggccgct ccttccccgc
     7801 cgcccgcggg cgcgcggcgc tacccaccgt gcctccgcgc gacgatgacg ccgcagacaa
     7861 cgtggcccag gccgaaaccg ctcccgagga ggaggtcgcc gtgggcgggg aagacgaggg
     7921 ccaggaggcc gagggcgagc agcgctcctc cgaagagagg aacgaccggc accgagagaa
     7981 gtcccgcggc gacgacggcg cacccgtaga gaagcagcca gatccccgga aggaaggcga
     8041 cggcggccga ggacgcgagc gcggccgtga gggcggcgcc cgcgacgagg ggcggcagga
     8101 gcgcgagcac gaagcgacgg gcggccgaga ccgaaggccg atggtcgaat cgcgcggcct
     8161 tgcgaaggag gccgaacgct ccgatcccga acgcgaggac ggcggcgccg gtccagatcg
     8221 cgagccactc acgcgtcgac gacgcccggg ccgcccaggc cgcggcggcg acgccgacgg
     8281 cgcccatggc gacgccgccc ttcccgggga cgcacgtgaa cgccgcggcc cggcccat
//
LOCUS       DAOMFY010000162         8292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2661_length_8292_cov_7.956295, whole genome shotgun sequence.
ACCESSION   DAOMFY010000162 DAOMFY010000000
VERSION     DAOMFY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_8292_cov_7.956295"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLB01_12775"
     CDS             complement(<1..372)
                     /locus_tag="PLB01_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HQQ78220.1"
                     /translation="MNRRDLLGAAAATAAVSSLPSFAFAADDRAKVYAEIEKRHGEAV
                     ARLQDWVRNPTIAAEGRGVETGCAQMMSLAKDAGFQTAVRMPTSGVPGVFATLDAGAP
                     RTVGLYVMYDVEEADPAEWEDA"
     gene            complement(402..1973)
                     /locus_tag="PLB01_12780"
     CDS             complement(402..1973)
                     /locus_tag="PLB01_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HQQ78221.1"
                     /translation="MTDRPTTLGELVAAGYAPKTVKDEIRDNLIAKLKAGENVFPGIL
                     GYEKTVVPALQNAILARHDIILLGLRGQAKTRILRALVNLLDEAIPVIAGSELNESPF
                     APFTAYGRRMAASAGDDLPVAWLGRDARYREKLATPDVTIADLIGDVDPVKAATLRKT
                     FADEDVIHYGIVPRTNRGIFAINELPDLQPRIQVGLLNILEERDLQIRGFPLRIPLDV
                     LMVFSANPEDYTNRGNIITPLKDRIASQILTHYPQDKKVASAITDQEAWTDRTLHGVT
                     VLLGEEVKLLVEEVAFVARRSELVDQSSGVSARLPIAARELLISNLERRALATGEAVV
                     APRLVDLFATLPAITGKVEMVFEGEQQGAEIVAKKLIGDAVKNLFEAQFPPVEGPRGR
                     TERPRRPRGDFEDDEGGGPTTLERAEARRGAAPAPPAGPYDAIAAWFADGKKIVLSDQ
                     TPFSEHLKTLESVAGLSDFVLKHAKPRDHYERAFLMELVLEGLVQNLRIAREDLDSTL
                     TYAELAKFNLMRSRG"
     gene            2013..2540
                     /locus_tag="PLB01_12785"
     CDS             2013..2540
                     /locus_tag="PLB01_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78222.1"
                     /translation="MDLRRVLGVVSSACGAAGARWAVIGGVAMNAYGHARTTFDFDVA
                     SEARLREDLLRRLQTEGFRLLNDVPAFSNLFHDDRDLGRLDFLWLDEKTSERVFAESR
                     TLPIWGENIPVASPEHLVAMKVQAVASSPTRVFRDGADLQFLLKVPGIDDNRVREFFE
                     GAGLLELLGRLRPRR"
     gene            2593..2724
                     /locus_tag="PLB01_12790"
     CDS             2593..2724
                     /locus_tag="PLB01_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78223.1"
                     /translation="MRKTDPGSLPWDVYCDFLKQFTPTKEQLEALPLSPDVPPFRLD"
     gene            complement(2783..3301)
                     /locus_tag="PLB01_12795"
     CDS             complement(2783..3301)
                     /locus_tag="PLB01_12795"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQQ78224.1"
                     /translation="MVSPAGLTPGTRAPSAAFSRPGGGTVTTEGLLAEANGLPLLLAF
                     FKTSCPTCRLAWPYLQRLHEAYGGTGVRVVGVSQNGAAESEDFFETFGKATFDLVLDP
                     EPRFAASNAFQVESVPHFVLISPAGLIEEIFSGWSKAKMEALGERLAAGRSLARIPVV
                     PPGDLVRDFQSG"
     gene            complement(3323..3739)
                     /locus_tag="PLB01_12800"
     CDS             complement(3323..3739)
                     /locus_tag="PLB01_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78225.1"
                     /translation="MASLVLFLLLLAEPVTASGPTMSAGPAGDSRTIVGEIVWVDVPS
                     RLVLIRESVKTTKVKGQPPARQTIAVSVAPDVAVTRGAKAVPLEDLKPKDHVTARYLA
                     TAAGAQALSFRVADAAPRPLASAPAAADGSAHAGGN"
     gene            complement(3888..5279)
                     /locus_tag="PLB01_12805"
     CDS             complement(3888..5279)
                     /locus_tag="PLB01_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ78226.1"
                     /translation="MTEKEPKATLLVVDDEKHQRESLTVILEDEGYRVAAAADGREAL
                     AKAAELRPEVVLTDLKMPGLTGMDVVKSLSSSAENALPPKVILVTAHGSVERAREAHK
                     LGAFDYMSKPVVADELLFRVERALEAFRLAEKALRLERRLKGDGLESIVGDSASMRDV
                     FRLVEKIAPTNSTVLIRGESGTGKELVARAVHARSNRSQKPFFALNCAAIPENLLESE
                     LFGHEKGSFTGADARKIGLFEAASGSTLFLDEIGDLTLPLQGKILRALQEKEVKRVGG
                     NETIPVDVRVVAATNRDLTAMMKAGQFREDLFYRLNVIPVELPPLRDRASDVRALVNR
                     FLEKANASHGTSVASVSPEAMDLLVRHGWPGNVRQLESVVERAVLLCEGNVVTPADLP
                     SEVRLNTAPAARPYGFDIPAEGIDIEEFERQLIVQAMEKSGWVIAKAARLLGLTYRTL
                     QYRLEKFGLKKPG"
     gene            complement(5296..6771)
                     /locus_tag="PLB01_12810"
     CDS             complement(5296..6771)
                     /locus_tag="PLB01_12810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ78227.1"
                     /translation="MALVRGVVMAAPEGFAGMRLSTRLGLTWAGILVLAAIAQTLVYF
                     QTRNETLDEADASYQAIAKAIEVGQTQIGPEGWKDPKVLEDYTAALGARGLRDIKVTD
                     EAGKPLFEPTPSAKRRARKSDGPKDIVISGVIGSGPTNRSLTVPIVVEGRLRGHVEIA
                     YNLENLRAQLEDNFRRRLFALLGVFALGLAVMLVITRNATRPLDQLADGAARVAEGHL
                     DVSVPVDRDDEVGRLAATFNRMTERLRERQELELRLAAAEKRAEIGHLASGLAHEIKN
                     PLNALSLGLDVLRRRHSPADPSAVAEYGGRIEALREEINRLASLINNFLAYGRPLTLT
                     FAPTDLAAVVARTLADLAETAERAHVTFAVRLPSSRPSVSADANLLKSSVWNLVQNGI
                     QSMERHGGTLAVTVDPEEGGSDGPPRRLVLSVTDEGPGLSETDLPRLFDPYFSKKEGG
                     VGLGLAMVKRIVEEHGGRVTAENRTDGRPGAVFRVSLPVAG"
     gene            complement(6747..7424)
                     /locus_tag="PLB01_12815"
     CDS             complement(6747..7424)
                     /locus_tag="PLB01_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQQ78228.1"
                     /translation="MADFPLSLVIGFLAGFLGAIPPGPLNVTVLRKSLHGRRRDAYRV
                     ASGGSAVDILVCLLIGLGLGWILEKVATNFWVRTALALFLVAYGLKLLLVDRRRDKLV
                     LAAGSAEPNVSGETSAPAQKPAGLRFVVTGLLQGAANPALFVNWTILISFLVSHRLFV
                     PSAISALGFALGVGAGVFAWFALLIELVDHWKDRAGAWVSRSTFLAGALLVAFGVFFT
                     WRSFVVS"
     gene            7483..8094
                     /locus_tag="PLB01_12820"
     CDS             7483..8094
                     /locus_tag="PLB01_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78229.1"
                     /translation="MRRLLAPACAAAALAGLSGAAQSGIEFEPKVGAANTRVTIRTTV
                     PAGGQVLFGGRPVNVIEEAKGIYSFQVPPTASGTAFVEIAKDGKPVAKSAVPFVVSGP
                     SVVSVPKLVGLKEAIDVFGYADTRPEGGGKPEPKARPILKFDDDEVLTIGDPAPFVLG
                     PAVQTGDLATAGRTSMSGTGLLITARPPKKKTPVPLPPPPPEN"
     gene            complement(8030..>8292)
                     /locus_tag="PLB01_12825"
     CDS             complement(8030..>8292)
                     /locus_tag="PLB01_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78230.1"
                     /translation="AGARITGVGIPGDGLYPESVVKGWAREAGAAYAELPSSHGHDGF
                     LLEPEAAGALLANALAKAPARVSSRVEAAGAGRGSSSWAAAR"
BASE COUNT         1234 a         2781 c         3009 g         1268 t
ORIGIN      
        1 cgcgtcctcc cactcggcgg gatccgcctc ctcgacgtcg tacatgacgt agaggccgac
       61 ggtcctcggc gcgcccgcgt cgagcgtcgc gaagaccccg gggacgccgg acgtcggcat
      121 ccggacggcg gtctgaaagc ccgcgtcctt cgcgagcgac atcatctgcg cgcagcccgt
      181 ctcgacgccg cggccctcgg cggcgatcgt cgggtttcgc acccagtcct ggaggcgcgc
      241 gacggcctcg ccgtggcgct tttcgatctc ggcgtagacc ttcgcgcggt cgtcggccgc
      301 gaaggcgaaa gaggggaggg aggacacggc ggcagtcgcg gcggccgcac cgaggaggtc
      361 gcgtctgttc atcggcatag gggggagtct aacgccgccg gctagcctcg cgaccgcatc
      421 aggttgaact tcgcgagctc cgcgtacgtg agcgtcgagt cgaggtcctc gcgcgcgatc
      481 cggaggttct ggacgagccc ttcgaggacg agctccatga ggaacgcgcg ctcgtagtgg
      541 tcgcgcggct tggcgtgctt gaggacgaag tccgagaggc ccgccacgga ttcgagcgtc
      601 ttcaggtgct cggagaacgg tgtctggtcc gagaggacga tcttctttcc gtccgcgaac
      661 cacgccgcga tcgcgtcgta cggtcccgcc ggcggcgccg gggcggctcc gcgccgggcc
      721 tcggccctct cgagcgtcgt cggaccgccg ccctcgtcgt cctcgaagtc gccgcggggg
      781 cgacgggggc gctcggtacg cccgcgcggg ccctcgacgg gcgggaactg cgcctcgaag
      841 aggttcttca cggcgtcgcc gatgagcttc ttggcgacga tctcggcgcc ctgctgctcg
      901 ccctcgaaca ccatctcgac cttgcccgtg atcgcgggga gcgtcgcgaa gaggtccacg
      961 aggcggggcg cgacgaccgc ctcgcccgtc gcgagcgcgc ggcgctcgag gttcgagatc
     1021 aggagctcgc gcgcggcgat cgggaggcgc gccgagacgc ccgaggactg gtcgacgagc
     1081 tcgctccggc gcgcgacgaa cgcgacttcc tcgacgagga gcttcacttc ttcgccaagg
     1141 agcacggtca cgccgtgcag ggtccggtcg gtccacgcct cctggtccgt gatcgcggag
     1201 gcgaccttct tgtcctgcgg gtagtgcgtg aggatctgcg acgcgatgcg gtctttcagc
     1261 ggcgtgatga tgttcccgcg gttcgtgtag tcctctgggt tcgcggagaa caccatgagg
     1321 acgtcgagcg ggatgcgcag agggaagccg cggatctgga ggtcgcgctc ctcgaggatg
     1381 ttcaggagcc cgacctggat gcggggctgc aggtccggca gctcgttgat cgcgaagatg
     1441 ccgcggttcg tacgcgggac gatcccgtag tggatgacgt cctcgtcggc gaacgtcttg
     1501 cggagcgtcg cggccttcac gggatccacg tcgccgatga ggtcggcgat cgtgacgtcc
     1561 ggcgtcgcga gcttctcgcg gtagcgcgcg tcgcgcccga gccacgccac gggcaggtcg
     1621 tcgcccgccg aggcggccat gcgccgcccg tacgccgtga acggcgcgaa cggcgactcg
     1681 ttcagttcgg aacccgcgat cacggggatc gcctcgtcga gcaggttgac gagggcgcgc
     1741 aggatgcgcg tcttggcctg gccacgcagg ccgaggagga tgatgtcgtg ccgcgcgagg
     1801 atcgcgttct ggagcgccgg gacgaccgtt ttctcgtagc cgaggatgcc ggggaagacg
     1861 ttctcgcccg ccttcagctt cgcgatgagg ttgtcgcgga tctcgtcctt gacggtcttg
     1921 ggcgcgtagc ccgccgcgac gagctctccg agggtggtcg ggcgatcggt catgagactc
     1981 ccttgccgca agcttacggg ataattggcc cgatggattt gaggcgggtg ctcggcgtcg
     2041 tgagctcggc ctgcggggct gcgggcgccc gctgggcggt gatcgggggc gttgccatga
     2101 acgcgtatgg ccacgcgcgt acgacgttcg acttcgacgt cgcttcggag gcgcggcttc
     2161 gcgaagacct tcttcgaaga ctgcaaaccg aagggttccg gctgttgaac gatgttcccg
     2221 cgttctcgaa cctgttccac gacgaccgcg acctcggccg actcgatttt ctctggctgg
     2281 acgagaagac cagcgaaagg gtctttgcgg aatccaggac ccttcccatc tggggagaga
     2341 acatcccggt agcctctccg gaacatctcg tcgcgatgaa ggttcaggct gtcgcgagct
     2401 cgccgacgcg cgtctttcgc gatggcgcgg atctgcagtt tcttctgaaa gtacccggga
     2461 tcgacgataa tcgggtccgt gagttcttcg aaggtgccgg actcctggag cttcttggac
     2521 gtctccggcc acggcgctga ggacttgggg gggcttcgac tcccgcggcc gacggccgaa
     2581 gagatggaag cgatgcggaa gaccgatccg ggctctctcc cgtgggacgt ctactgcgac
     2641 ttcctgaagc agttcacgcc gacgaaggag cagctcgagg cgttgccgct ttcgccagac
     2701 gtcccgccgt tccggctcga ctgaagcgcg gcgctttcgg gtttcggact gacctcacgg
     2761 gtcagtgctg gttgcggccc agtcaacccg actggaagtc gcgcacgagg tcgcccggcg
     2821 gcacgacggg gatccgagcc agggagcgtc cggcggcgag acgttcgccg agcgcctcca
     2881 tcttcgcctt cgaccagccc gagaagattt cttcgattaa tcccgcggga gagatcagga
     2941 cgaagtgagg aacggattcc acctggaagg cgttcgaggc cgcgaagcgc ggctcaggat
     3001 ccaggaccag atcgaacgtg gctttcccga acgtctcgaa gaaatcttcg ctctcggcgg
     3061 ccccgttctg cgagacgccg acgacgcgca cgcccgtgcc gccgtacgcc tcgtgcaagc
     3121 gctgaaggta cggccacgcc aggcggcacg tcgggcagga cgtcttgaag aacgcgagga
     3181 ggagcggaag gccgttcgcc tccgcgagga ggccctccgt cgtgacggtt ccgccgccgg
     3241 ggcgcgagaa ggcggcggac ggagcgcgag tcccgggagt caaccctgcg ggcgagacca
     3301 tgcccgagag tctagaccgg gctcagttgc cgcccgcgtg cgccgagccg tcggcggccg
     3361 ccggagcgga ggcgaggggg cgcggcgcgg cgtccgcgac gcggaacgag agcgcctgcg
     3421 cgccggcagc cgtcgcgagg taacgcgcgg tcacgtggtc cttcggcttg aggtcctcga
     3481 gcggcacggc cttcgcgccg cgcgtgacgg cgacgtccgg cgcgacgctg acggcgatcg
     3541 tctgccgcgc gggcggctgg cccttcacct tcgtcgtctt gaccgactcg cggatcagga
     3601 cgaggcgcga cggaacgtcg acccacacga tctcgccgac gatggtgcgc gagtctccgg
     3661 ccggaccggc agacatcgtc gggccggacg cggtcaccgg ttcggcgaga aggagcagaa
     3721 agaggacgag gctcgccacg ccttccggct tgcaggacac gtgccgaccg gccggcgccc
     3781 cccatcggcg gggtttgcgg gggtttgggc gcccgaatgg catttcgggg gcccgtcggc
     3841 cggccccgat tgcacgattc ggggcaagag atctaccgga tcaggtctca gccgggcttc
     3901 ttgaggccga acttctcgag gcggtattgc aacgtccggt aggtgagacc gaggaggcgc
     3961 gcggccttcg cgatcaccca tcccgatttt tccatcgcct ggacgatgag ctgtcgctcg
     4021 aactcctcga tgtcgatccc ctcagccggg atgtcgaaac cgtagggccg cgcggccggc
     4081 gccgtgttga ggcggacctc ggacggcagg tccgccggcg tcacgacgtt tccttcgcag
     4141 aggaggacgg cgcgctcgac gacgctctcg agctgccgca cgttccccgg ccagccgtgc
     4201 cgcacgagga ggtccatcgc ctcgggcgag acggaggcga cggatgtccc gtgcgaggcg
     4261 ttcgccttct cgaggaagcg gttcacgagg gcgcgcacgt cggaggcacg gtcgcggagg
     4321 ggcggcagtt ccaccgggat cacgttcagg cggtagaaga ggtcctcgcg gaactggccc
     4381 gccttcatca tcgccgtgag atcgcggttc gtggccgcga cgacgcgcac gtcgacggga
     4441 atcgtttcgt ttccgcccac gcgcttgacc tccttctcct ggagggcgcg caggatcttg
     4501 ccctgtagcg ggagcgtgag gtcgccgatc tcgtcgagga acagcgtcga cccgctcgcg
     4561 gcctcgaaga ggccgatctt gcgggcgtcg gctcccgtga acgacccctt ctcgtgtccg
     4621 aagagctcgc tctcgaggag gttctccggg atcgcggcgc agttcagggc gaagaacggc
     4681 ttctgggagc ggttggagcg cgcgtggacc gcgcgcgcga cgagctcctt gcccgttccg
     4741 gactcgccgc ggatgaggac cgtcgagttc gtcggcgcga tcttctcgac gaggcggaag
     4801 acgtcgcgca tcgaggcgct gtccccgacg atcgactcga ggccgtcgcc cttcaggcgc
     4861 cgctcgagcc gcagggcctt ctccgcgagc cggaacgcct cgagggcgcg ctcgacgcgg
     4921 aagagcagct cgtcggccac gacgggtttc gacatgtagt cgaacgcgcc gagcttgtgg
     4981 gcctcgcgcg cgcgctcgac agagccgtgc gccgtcacga ggatgacttt cgggggcagg
     5041 gcgttctccg cggaggagct caggctcttg accacgtcca tgcccgtgag gccgggcatc
     5101 ttgaggtccg tgaggacgac ttcgggccgg agctcggcgg ccttcgcgag cgcctcgcgc
     5161 ccgtcggccg cggcggcgac acggtacccc tcgtcctcga ggatgacggt cagcgactcg
     5221 cgctggtgct tctcgtcgtc cacgacgagg agcgtcgcct tcggttcttt ctcggtcacg
     5281 gggcggagat taccctcagc cggccacggg gagcgaaaca cggaagacgg cgcccggtcg
     5341 tccgtccgtc cggttctcgg ccgtcacgcg gccgccgtgt tcctcgacga tgcgcttgac
     5401 catcgcgagg ccgaggccga cgccgccctc cttcttcgag aagtacgggt cgaagaggcg
     5461 cgggaggtcc gtctcggaga gcccggggcc ctcgtccgtg acggacagga cgaggcggcg
     5521 cgggggaccg tcggaaccgc cctcttccgg gtcgacggtg acggccagcg tgccgccgtg
     5581 gcgctccatc gactggattc cgttctggac gaggttccag accgacgact tcaggaggtt
     5641 cgcgtccgcc gagacgctcg gcctcgaaga gggaagccgc accgcgaacg tcacgtgggc
     5701 gcgctccgcg gtttcggcga ggtcggcgag ggtccgcgcg acgacggccg cgaggtcggt
     5761 cggcgcgaac gtgagcgtga gcggccgccc gtaggcgagg aagttgttga tcagggaggc
     5821 gaggcggttg atctcctcgc ggagggcctc gatccgcccg ccgtactcgg cgacggcgga
     5881 agggtccgcg ggggagtgcc ggcgcctgag gacgtcgagc ccgagcgaga gcgcgttcag
     5941 ggggttcttg atctcgtgcg cgaggcccga cgcgaggtgg ccgatctcgg cccgcttctc
     6001 ggcggcggcg aggcgcagct ccagttcctg ccgttcgcgc agcctctccg tcatccggtt
     6061 gaacgtcgcg gcgaggcgtc cgacctcgtc gtcgcgatcc acgggcacgc tgacgtcgag
     6121 gtgcccttcg gcgacgcgcg cggcgccgtc ggcgagctgg tcgagcggtc gcgtcgcgtt
     6181 gcgcgtgatg acgagcatga cggcaaggcc gagcgcgaac acgccgagga gcgcgaacag
     6241 ccgccgccgg aagttgtcct cgagctgggc gcggaggttc tcgaggttgt atgcgatctc
     6301 gacgtggccg cgcaggcggc cctcgacgac gatcgggacg gtgaggctcc ggttcgtcgg
     6361 gcccgagccg attacgccgg agatcacgat gtccttcggc ccgtcggact tcctcgcgcg
     6421 gcgcttcgcc gagggcgtcg gctcgaagag cggcttgccc gcctcgtccg tgaccttgat
     6481 gtcgcgcagc ccgcgggcgc cgagggcggc cgtgtagtcc tcgaggacct tcgggtcctt
     6541 ccagccctcc ggcccgatct gcgtctggcc gacctcgatc gccttcgcga tcgcctggta
     6601 cgacgcgtcc gcctcgtcga gcgtctcgtt gcgcgtctgg aagtagacga gcgtctgcgc
     6661 gatcgccgcg aggacgagga tgcccgccca cgtgaggccg agccgggtcg agaggcgcat
     6721 gccggcgaaa ccctcgggcg ccgccatcac gacaccacga acgagcgcca cgtgaagaag
     6781 acgccgaagg ccacgaggag ggcgccggcc aggaacgtgg agcgcgagac ccacgcgccg
     6841 gcccggtcct tccagtggtc cacgagctcg atcagcagcg cgaaccaggc gaagacgccc
     6901 gcgccgacgc cgagcgcgaa gccgagcgcg gagatcgccg acggcacgaa gagccggtgg
     6961 ctcacgagga aggagatgag gatcgtccag ttcacgaaga gcgcggggtt cgccgcgccc
     7021 tgcaggaggc ccgtgacgac gaaccggagg cccgcgggct tctgcgccgg agcggaagtc
     7081 tcgccggaga cgttcggctc ggcggagccc gccgcgagca cgagcttgtc gcggcgccgg
     7141 tccacgagga gcagcttcag tccgtacgcc acgaggaaga gcgcgagagc cgtccgcacc
     7201 cagaaattcg ttgcgacctt ctcgaggatc cagccgagtc cgagcccgat caggaggcag
     7261 acgaggatgt cgacggccga accgccggac gcgacgcgat aggcgtcgcg gcgccgcccg
     7321 tgcaggctct tgcgcaggac cgtgacgttc agcggacccg gagggatcgc gccgagaaac
     7381 cccgcgagaa acccgatcac gagggacagc gggaagtccg ccacggcgcg gtatcgtctc
     7441 acgccgtttg cgggtcccgg cgaggaagcg tacattgggg gtgtgagacg tcttctcgct
     7501 cccgcctgcg ccgctgccgc cctcgccggg ctctccggcg ccgcccagag cgggatcgaa
     7561 ttcgagccga aggtcggcgc ggcgaacacg cgcgtcacga tccgcacgac cgtgcccgcg
     7621 ggcgggcagg tcctcttcgg cggcaggccc gtgaacgtca tcgaggaggc gaaggggatc
     7681 tacagcttcc aggtcccccc gacggcctcc ggcacggcgt tcgtcgagat cgcgaaggac
     7741 ggaaaacccg tcgcgaagag cgcggtcccg ttcgtcgtct cgggcccgtc ggtcgtttcg
     7801 gtcccgaaac tcgtcggcct caaggaagcc atcgacgtct tcgggtacgc ggacacgcgc
     7861 cccgagggcg gcggcaaacc ggagccgaaa gcgcggccga tcctgaagtt cgacgacgac
     7921 gaggtcctca cgatcggcga cccggcgccg ttcgtcctcg gcccggccgt ccagacgggc
     7981 gacctcgcga ccgccggcag gaccagcatg tcgggcacgg gcctcctcat caccgcgcgg
     8041 ccgcccaaga agaagacccc cgtcccgctc ccgccgcctc cacccgagaa ctgacgcgcg
     8101 cgggagcctt cgcgagcgcg ttcgcgagga gcgcgccggc ggcctccggt tccaggagga
     8161 acccgtcatg cccgtgcgac gaggggagct cggcgtacgc cgcgccggcc tcgcgcgccc
     8221 agcccttcac gacgctttcc gggtagagcc cgtcgcccgg aatccccacg cccgtgatcc
     8281 gcgcccccgc gg
//
LOCUS       DAOMFY010000163         8247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2686_length_8247_cov_6.687378, whole genome shotgun sequence.
ACCESSION   DAOMFY010000163 DAOMFY010000000
VERSION     DAOMFY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2686_length_8247_cov_6.687378"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2677)
                     /locus_tag="PLB01_12830"
     CDS             complement(<1..2677)
                     /locus_tag="PLB01_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78231.1"
                     /translation="MRTSDLARVATLELAPGESQIGSGAIDTVHGFAYVGTYTAPGSV
                     IKIRLSDFTRVGSLMLADGENYLRTAVLDPSGDFAYFGTEPVFGPIDVVKVRLSDFSR
                     VGALRLPAPAQFGGTTATMDARGISAYVTNSDTVFRLRLADFTASGSWRPASSGGPFT
                     CSVLDPTGTFGYFGTASSLSTLYKVRLSDLTEQGELYDVASHGWYTASVDPGGLFAYL
                     GAFGAITRIRLSDFKSLGTTILSSAPGQPTAAVVDTALGYLVMETWGSKSQIVRITVP
                     GFVESGALGLRIGENRVSSVVIDSSAGFAYFGTYTSPGLIVKVRLSDFKRVATLTLPP
                     GEDGLTSAVIDVAGGFAYFGTYTDPGIVIKIRLSDFTRVSATTLGPLESYLYSAFLDS
                     PAGFAYFGTETSRLPAGSSGLLVKVRVSDLARIGSRDVTGGPGSVNSAAPAPEGGFAY
                     LGVESAGALGIARVRLSDFTLDGILSLPDLYSFLSAIVLDPISGFAYAVSSDMPSRVA
                     RIRLADFTSTGGLNLPDAARWVSTGVIDPAGGWAYFASYGDVYHPERVFRVRLSDFTN
                     AGSLSLDDQHYYSAGVNVGAIDPSTLLGYFATDERPSRVFKVDLGRPGVATAAVSGGG
                     EVCPGGGGTIYAYLSGVPPWSVTWSDGAQYAGLTVSPQTRTVTVSAPTIYSVTSVSDA
                     NGPGLASGVATFSIFPPTPPPTITGPLSAFSGASGLTASVPVHAASSYLWYGDSDAVI
                     TSGSSSSQIMFKAATNGPTILHASEFGPCQSAWADLVIPVQVATPPLSLQPLTPCRIF
                     DTRNATGPAAASPALAAAADRILSVTSRCGIPATASAISVNAAVTQPAAAGNLSFHAG
                     NEPTNGSLALNFSPGQTRASNAIVRLASDGSGTIGV"
     gene            complement(3073..3405)
                     /locus_tag="PLB01_12835"
     CDS             complement(3073..3405)
                     /locus_tag="PLB01_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78232.1"
                     /translation="MAIDNMYRRRRLGPPLGVRMALSIPPSVFFPTLGFLYMAFLDHP
                     RRLRPALLYVFAPITALGSTKDIGLAAMLVLLDVLVWMAVLNGLLTGIGWLFEKPDPH
                     ARRPPPPH"
     gene            complement(3409..3747)
                     /locus_tag="PLB01_12840"
     CDS             complement(3409..3747)
                     /locus_tag="PLB01_12840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQQ78233.1"
                     /translation="MAAVLRGDVVWADLNPVRGSEQAGLRPVLVLSADVFNRHSGTVV
                     AVALTSQAPRAGFPLTLPLSDPKLPKKSWVKIGQVRTLSTERLGKRLARATPDELAQV
                     LEGLNEILGG"
     gene            complement(3732..3971)
                     /locus_tag="PLB01_12845"
     CDS             complement(3732..3971)
                     /locus_tag="PLB01_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HQQ78234.1"
                     /translation="MSTAKVAVSLDRRTLTRVDRLVRRGLFPSRSRLVQDSIEEKLGR
                     LDRARLARECAKLDRGVERALAEEGLEPVAGWPPY"
     gene            complement(4023..6905)
                     /locus_tag="PLB01_12850"
     CDS             complement(4023..6905)
                     /locus_tag="PLB01_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ78235.1"
                     /translation="MRFVRSAALVLTSLLATRGNAEVAKPASPAAAKPAAKPAADILP
                     FKATEKTLPNGLKVIVVPTGFPNIVSLQIPVKTGSRNEVESGKSGFAHFFEHMMFRGT
                     KAYPPARYQEIVTRAGARQNAYTTDDYTNYHTTFPKEDLETMLKIEADRFQNLEYGES
                     EFKTEARAVLGEYNKNSANPISKLYEVTRDKAFNVHTYKHTTMGFLKDIEDMPNQFAY
                     SRTFFDRWYRPEHTTIIVAGDVDPAKTIALVEKYWSGWKRGGKTLPIPQEPPAKGPVT
                     AHVPWPSDTLPWVVVAFHGPAFSETKKDLPALDALFDLTFGPTSDLYKKLVEKEQKVD
                     SFQPSVDVNEDPELVGVFTRLKKGTDPVYVRDQILAAMAALRETPVDAKRLAEVKSNS
                     RYGLVRGLDDTERIAGLLARFVRLRRSYGTLNQYYRLIDALTPADLQAAAKTYLTDTN
                     LVLTTLSKDPMPEAMATLAPLASYGAAAKGGPSGPDVAWIEQKSPLPRLEVKLMFAAG
                     SAHDPAGKEGLAALTARMIADGGSQDLRIDEIKRALFPVAASFDAGCDREMTIFTGVV
                     HRDAWKTFADVALPQLVAPGFREEDFRRIKDALKNALVQNLRSNNDEELGKERLQQRV
                     FAGTPYGHPTLGSVASLNAITLDDVKAFAQKAYTRAALTVGVAGDVPAEFAARVKADA
                     AKLPAGPALPAPAGVAGRKPSGIDVDIVKKETRSTAISFGFPIDVTRAHPDFAALSVA
                     RAWLGEHRSSSSHLYQRIREVRGMNYGDYAYIEAFPGGMYRMLPEPNVARRAQLFEVW
                     IRPVMPQNAHMALRVAIFELDKLVSEGLSEEEFQTTRDYLMKNVYLLTATQDHALGTA
                     LDQKWYGLPDYVTDMRAKLGKLTRADVNAAVKRHLQAKNLSIVMVTKDAEALKAALVA
                     DEFSPITYDGEKPKALLDEDKVIGALKLGVKAEAVTITPVDDVFAK"
     gene            complement(6974..7792)
                     /locus_tag="PLB01_12855"
     CDS             complement(6974..7792)
                     /locus_tag="PLB01_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78236.1"
                     /translation="MRLRTPLLSFLFAAGSALGNAPAGFGLEVLVDGAARPEYPARGT
                     VYVEALRGRDFSLRITNPLGVRVAVALSVDGLNTIDAKHTTARDARKWILDPYQTVVI
                     PGWQVSGDTSRKFYFTGEKRSYGAQLGQTSNLGTIEAVFFREKQPYRTGFWGGRKEEE
                     KNSANERRKDAADAGSPEPQAGASRDLSKQSPAPSSVSSLSDELAATGIGRRTRFDVT
                     RVDVDLEDAPAMSVRLRYEFRPQLVALGVLREPRDPVARREAGRGFSDFCPEPN"
     gene            complement(7842..>8247)
                     /locus_tag="PLB01_12860"
     CDS             complement(7842..>8247)
                     /locus_tag="PLB01_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQQ78237.1"
                     /translation="CRGRRRRRCRARCPSGEAVAPLGAGVGFLTGLLGVGGGFLIVPA
                     LVLAAGLAMPLAVGTSLVVIAINSAAGFAGHIGDQNVSYGYAAAFTGVCVVGALVGER
                     LGRNVPAARLRKWFGVFVIAVGLVLLAKNLAA"
BASE COUNT         1347 a         2626 c         2968 g         1306 t
ORIGIN      
        1 ggacgccgat ggtccccgat ccgtcgctcg caagccggac gattgcgttg ctggctcgtg
       61 tctggccggg gctgaagttc agagccaagg acccgttcgt cggctcgttg cccgcgtgga
      121 aggagagatt cccggccgcg gcgggctggg tgaccgcggc gttcacggag atcgccgagg
      181 ccgtcgcggg aatcccgcag cgactcgtga cggagagaat tcgatccgca gcggccgcga
      241 gggcgggtga ggcggcggcg ggtcccgtgg cgttgcgcgt gtcgaagatg cggcaggggg
      301 tgagcggctg gagcgagagg ggcggtgtcg cgacctggac gggaatgaca aggtccgccc
      361 acgcggactg gcaaggcccg aattcgctcg cgtggaggat cgtcgggccg ttcgtcgccg
      421 ctttgaacat gatctggctc gacgaacttc ctgacgtaat gacggcgtcg gaatcgccgt
      481 accagaggta ggagctcgcc gcgtgcaccg gaacgctcgc cgtcaggccc gacgcgcccg
      541 agaatgccga gagcgggccg gtgatcgtcg gagggggcgt cggaggaaag atcgagaacg
      601 tggccacccc gctcgcgagg cccgggccgt tcgcatcgga cacggacgtc accgagtaga
      661 tcgtaggtgc gctcaccgtc accgtgcgcg tctgggggct gaccgtgagg cccgcgtatt
      721 gcgccccgtc ggaccatgtc accgaccacg ggggaacgcc gctgaggtag gcatagatcg
      781 tccccccgcc ccccggacag acctcgccgc cgccgcttac ggcggccgtc gccaccccgg
      841 gtctcccgag atcgaccttg aagacgcggg aaggccgctc gtccgtcgcg aagtacccga
      901 ggagggtcga gggatcgatg gctccgacgt tcacacccgc gctgtaatag tgctggtcgt
      961 cgagcgacag ggaacccgcg ttcgtgaaat cggagagtcg cacccgaaac acacgttccg
     1021 ggtggtagac atcgccatac gacgcgaagt aagcccatcc acccgccgga tcgatgactc
     1081 ccgtcgagac ccaccgggcc gcgtccggga gattcagtcc accggtggat gtgaagtcgg
     1141 ccagtcggat cctcgccacc cttgagggca tgtcggacga gaccgcatag gcgaagccgg
     1201 agatcgggtc gagcacgatc gcgctcagga aggagtacag gtccggcagc gacaggatgc
     1261 cgtcgagtgt gaagtcggaa agacgcaccc gcgcgattcc cagcgccccc gccgattcga
     1321 ccccgagata cgcgaatccg ccctcgggcg ccggggctgc tgagttcacg gacccggggc
     1381 cgcccgtcac gtcccgtgaa ccgatgcggg cgagatccga gacgcggacc ttgacgagga
     1441 ggccggagct ccctgccgga agcctgctcg tctccgttcc gaagtaggcg aaccccgcgg
     1501 gggagtcgag gaaagcggaa tacagatagg actccagagg cccaagtgtc gttgcgctga
     1561 cccgcgtgaa gtccgaaagc cggatcttga tcacgatgcc ggggtccgtg taggtgccga
     1621 agtaggcgaa gccgccggca acgtcgatca cggccgacgt gagaccgtcc tcgccaggcg
     1681 gaagagtgag ggtcgccacc cgcttgaagt ccgaaaggcg cactttcacg atgaggcccg
     1741 ggctcgtgta ggttccgaag tacgcgaacc ccgcggaaga gtcgatcacg acggaggaga
     1801 cgcggttctc gccgatcctg aggccgagcg ccccggactc cacgaacccg gggaccgtga
     1861 tgcggacgat ctgcgacttg cttccccaag tctccatgac gagatagccg agagccgtat
     1921 cgacgaccgc cgcggtcggc tgccccgggg ccgagctcag gatggtggtc ccgagcgact
     1981 tgaaatcgga gagccggatt cgagtaatgg ctccgaacgc gcccagatat gcgaagaggc
     2041 caccgggatc gacactcgcg gtgtaccaac cgtggcttgc aacgtcatac agctctccct
     2101 gctcggtgag gtcggagagg cggaccttgt aaagagtcga cagggacgag gcggtcccga
     2161 aatagccgaa ggtcccggtc ggatcgagga cggaacacgt gaatgggccc ccggaggacg
     2221 ccggccgcca cgagccagaa gcggtaaagt cggcaagccg taggcggaac accgtgtcgc
     2281 tgttggtgac gtacgcggat ataccccgcg cgtccatcgt ggccgtcgtg cctccaaact
     2341 gggccggtgc cggcagccgc aacgctccga cacgcgagaa gtctgaaagg cggaccttca
     2401 cgacgtcgat ggggccgaag accggttcgg tgccgaagta ggcgaagtcg cccgagggat
     2461 cgaggacggc ggtccggagg tagttctcgc cgtcggccag catcagggaa ccgacgcgcg
     2521 tgaaatccga gagcctgatc ttgatgaccg atccgggcgc ggtgtaggtc cccacgtacg
     2581 cgaatccgtg gaccgtatcg atggctcccg agccgatctg actctcgccg ggagcgagct
     2641 cgagcgtcgc gacgcgcgcc aggtccgaag tccgcacttt gacgatcgtc gccgagccga
     2701 cctcgggatc ctgggagaag gcgaaatacg cgaacccggc ctgcgaatcg acgaacgctg
     2761 ctctcgcgtc tacttcaccg gcgtcgaagt ggagagtcgt cggtcgatcg ccgaaagcga
     2821 ggaggttgcg actcccattt gacgggaccg gagggcgtac gaccggttcc gtgatcgggc
     2881 cgcgtccggg aagaagatcg ggcagcgaac tcgttcgagg ggcgcgggaa aacgtcctgt
     2941 ccgcagccgg ttccgatcgt gcgagcgtga ccgacccgat cgccgcggcg agcgacagtg
     3001 cattgataca aagacgcaac tgcttgcaag cgatttgcat acttccaaga tagggcggcc
     3061 cctctcgggg agtcaatgag gcggcggagg tctccgggca tgcgggtccg gcttctcgaa
     3121 cagccagccg atcccggtca ggaggccgtt gagcacggcc atccagacga gcacgtcgag
     3181 gaggaccagc atggccgcga ggccgatgtc cttcgtagat ccgagcgctg tgatcggagc
     3241 gaagacgtag agcagggccg gacgcagcct tcgcgggtgg tcgaggaagg ccatgtagag
     3301 gaagccgagc gtcgggaaga agaccgacgg cgggatcgac agcgccatcc ggacgcccag
     3361 cggcggcccg aggcgccgcc gccggtacat gttgtcgatt gccacgcgtc aaccgccgag
     3421 gatttcgttg agaccttcga gaacctgcgc gagttcgtcc ggagtcgccc gcgcgagtcg
     3481 cttgccgagc cgctccgtcg agagagtgcg gacctggccg atcttcaccc aggacttctt
     3541 ggggagcttc gggtcggaga ggggcagggt gagagggaac ccggcgcgag gcgcctggct
     3601 cgtcagcgcg acggccacga cggtcccaga gtggcggttg aagacgtcgg cgctcaggac
     3661 gaggacgggg cgaaggcccg cctgctcgct tccgcggacc gggttcaggt ccgcccagac
     3721 gacgtcgccc ctcagtacgg cggccatccc gccaccggct ccagcccttc ctcggcgagc
     3781 gcacgctcga cgccgcgatc cagcttcgcg cattcgcgcg cgagccgggc gcggtcgaga
     3841 cgtccgagct tctcctcgat ggagtcctgc acgagcctgc tgcggctcgg aaaaagtccg
     3901 cgccgaacga gccggtcgac gcgcgtcagg gtgcggcggt ccagcgacac ggcgactttt
     3961 gcggtgctca tgggtatgac tctaggtcat acccacgagc ctgtcaacgg ttcagccggc
     4021 gcctacttcg cgaacacgtc gtcgaccggg gtgatcgtca cggcttcggc cttgacgccc
     4081 agcttgagcg cgccgatcac cttgtcctcg tcgaggagcg ccttcggctt ctcgccgtcg
     4141 tacgtgatcg gcgagaactc gtcggcgacg agcgccgcct tcagcgcctc ggcgtccttc
     4201 gtgaccatca cgatcgacag gttcttcgcc tgcaggtgcc gcttgacggc tgcgttcacg
     4261 tcggcgcggg tgagcttccc gagcttcgcc cgcatgtccg tgacgtagtc cgggaggccg
     4321 taccacttct ggtcgagcgc cgtgccgagg gcgtggtcct gcgtcgccgt gaggaggtag
     4381 acgttcttca tgaggtagtc gcgcgtcgtc tggaattcct cctcggagag gccctcgctc
     4441 acgagcttgt ccagctcgaa gatcgcgacg cggagtgcca tgtgcgcgtt ctgcggcatg
     4501 acggggcgga tccagacctc gaagagctga gcgcggcggg ccacgttcgg ctcgggaagc
     4561 atccgataca tcccgcccgg gaaggcctcg atgtacgcgt agtcgccgta attcatgccg
     4621 cgcacctcgc ggatgcgctg gtagaggtgg gagctggacg accggtgctc gccgagccag
     4681 gcgcgcgcga ccgagagggc cgcgaagtcg ggatgcgcgc gggtcacgtc gatcgggaag
     4741 ccgaacgaga tcgccgtgga gcgcgtctcc ttcttcacga tgtcgacgtc gatgcccgag
     4801 ggcttccggc cggcgacgcc cgcgggcgcc ggcagcgcgg ggccggccgg gagcttcgcc
     4861 gcgtcggcct tgacccgggc cgcgaactcg gccggaacgt ctcccgcgac gccgaccgtg
     4921 agcgcggcgc gcgtgtacgc cttctgcgcg aaggccttga cgtcgtcgag cgtgatcgca
     4981 ttgagagagg cgaccgagcc cagcgtcgga tggccgtacg gcgtgcccgc gaagacgcgc
     5041 tgctggaggc gctccttgcc gagctcttcg tcgttgttgc tgcggaggtt ctggacgagg
     5101 gcgttcttca gggcgtcctt gatccggcgg aagtcctcct cccggaagcc gggcgccacg
     5161 agctgcggga gagcgacgtc ggcgaacgtc ttccaggcgt cccggtggac gacgccggtg
     5221 aagatcgtca tctcgcggtc gcatccggcg tcgaagctgg cggcgacggg gaacagcgcc
     5281 ctcttgatct cgtcgatccg gaggtcctgg gatccgccgt ccgcgatcat gcgggccgtg
     5341 agggcggcga ggccttcctt gccggccggg tcgtgcgccg agcccgcggc gaacatgagc
     5401 ttgacttcga ggcgcgggag cggggatttc tgctcgatcc acgcgacgtc gggcccggac
     5461 gggccgccct tcgcggccgc tccgtagctt gccagcggcg cgagcgtcgc catcgcctcg
     5521 ggcatcgggt ctttcgagag ggtcgtgagg acgaggttcg tgtcggtcag gtacgttttc
     5581 gcggcggcct ggaggtcagc cggcgtcagg gcgtcgatga ggcggtagta ctggttcagc
     5641 gtcccatacg agcggcgcag gcgcacgaag cgggcgagga ggccggcgat gcgctccgtg
     5701 tcgtcgaggc cgcgcacgag gccgtagcgc gagttcgatt tcacctcggc cagccgcttc
     5761 gcgtcgacgg gcgtttcgcg gagggccgcc atcgcggcga ggatctggtc gcggacgtag
     5821 acgggatccg tccccttctt caggcgcgtg aagacgccga cgagctcggg atcctcgttg
     5881 acgtcgacgc tcggctggaa cgagtcgacc ttctgctcct tctcgacgag cttcttgtag
     5941 aggtccgacg tggggccgaa cgtgaggtcg aacagggcgt cgagggcggg caggtccttc
     6001 ttcgtctccg agaaggccgg gccgtggaac gcgacgacga cccacgggag cgtgtcggac
     6061 ggccacggca cgtgggcggt gaccgggccc ttcgcgggcg gctcctgcgg gatcgggagc
     6121 gtcttcccgc cccgcttcca gccgctccag tacttctcga cgagggcgat cgtcttcgcc
     6181 gggtcgacgt cgccggcgac gatgatcgtc gtgtgctcgg gccggtacca gcggtcgaag
     6241 aacgtcctcg agtacgcgaa ctggttgggc atgtcctcga tgtccttgag gaagcccatc
     6301 gtcgtgtgct tgtacgtgtg gacgttgaag gccttgtcgc gcgtgacctc gtagagcttc
     6361 gaaatcgggt tcgcgctgtt cttgttgtac tcgccgagga cggcgcgcgc ctcggtcttg
     6421 aactcggact ctccgtactc gaggttctgg aagcggtcgg cctcgatctt cagcatcgtc
     6481 tcgaggtctt ccttcgggaa cgtcgtgtgg tagttcgtgt agtcgtccgt cgtgtacgcg
     6541 ttctggcggg cgcccgcgcg cgtgacgatc tcctggtacc tggcgggggg atacgccttc
     6601 gtgccgcgga acatcatgtg ctcgaagaag tgggcgaacc cggacttgcc ggactcgacc
     6661 tcgttccgcg acccggtctt cacgggaatc tggagcgaga cgatgttcgg gaagccggtc
     6721 gggacgacga tgaccttcag gccgttcggg agggtcttct ccgtcgcctt gaagggcagg
     6781 atgtcggcgg cgggcttcgc ggccggcttt gcggcggcgg gggaggctgg ctttgcgacc
     6841 tcggcgtttc cccgggtcgc caggagggag gtgaggacga gggcagcgga gcggacgaag
     6901 cgcatgcggg gctctccttc gtagacggaa tctatcccct acgcgtacga aggagagccc
     6961 gaattgtttc gaatcagttt ggttcggggc agaagtccga aaacccccgg ccggcctccc
     7021 ggcgggcgac cgggtcacgc ggctcccgga ggacgccgag ggcgacgagc tgcggccgga
     7081 attcgtagcg cagccggacc gacatcgcgg gggcgtcctc gaggtccacg tccacgcgcg
     7141 tcacgtcgaa cctcgtccgg cggccgatgc cggtggcggc gagctcgtcg gagagagagg
     7201 agacggaaga gggagcgggg gattgcttcg aaaggtcgcg ggaagcgcct gcctgcggct
     7261 cgggcgaacc cgcgtccgcg gcgtctttcc ttctttcatt cgcagaattc ttctcttctt
     7321 cctttctccc tccccagaag cccgtccggt acggctgctt ctcgcggaag aacaccgcct
     7381 cgatcgtccc gagattcgac gtctgcccga gctgagcgcc gtaggagcgc ttctcgcccg
     7441 tgaagtagaa cttccggctc gtgtcgccgg acacctgcca gccggggatc acgacggtct
     7501 ggtacgggtc gaggatccac ttgcgtgcgt cccgcgcggt cgtgtgcttc gcgtcgatgg
     7561 tgttcaggcc gtcgacggac agcgcgacgg ccacgcgcac gccgagcggg ttcgtgatcc
     7621 ggagcgagaa gtcgcggccg cggagcgcct cgacgtagac cgtgccgcgg gcggggtact
     7681 cggggcgggc cgcgccgtcc acgaggacct cgaggccgaa gccggcggga gcgtttccga
     7741 gagcggaacc ggcggcgaac aggaacgaca ggagcggggt gcgaagtcgc atggcgatct
     7801 ccttcgcacc gttggacccg gcccgtgccg ggaggttccc gtcaggccgc gaggttcttc
     7861 gcgaggagga cgaggccgac cgcgatgacg aagacgccga accacttccg cagcctcgcg
     7921 gcgggcacgt ttcgcccgag acgctccccg accagcgcgc cgacgacaca gacgccggtg
     7981 aacgccgcgg cgtagccgta cgacacgttc tggtccccga tgtggcccgc gaatcccgcc
     8041 gcggagttga tcgcgatcac gacgagcgac gtgccgacgg cgagcggcat cgcgaggccg
     8101 gccgcgagga cgagcgcggg gacgatgagg aacccgccgc cgactccgag caggccggtg
     8161 aggaacccga cgcccgcgcc gagcggggcg acagcttccc ccgaagggca ccgcgctcgg
     8221 catcgccttc tccggcggcc tcgacac
//
LOCUS       DAOMFY010000164         8229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2698_length_8229_cov_8.472229, whole genome shotgun sequence.
ACCESSION   DAOMFY010000164 DAOMFY010000000
VERSION     DAOMFY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2698_length_8229_cov_8.472229"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLB01_12865"
     CDS             complement(<1..592)
                     /locus_tag="PLB01_12865"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HQQ78238.1"
                     /translation="MPTPRCNLRTSTDYHVHTARCGHAGGAMRAYVETALARGLSEIA
                     FTDHVPLYFLPGDDPDPGIAMTRAELPGYVEEVQALSEEFAGRIDVLLGLEADYAEGH
                     EAVLAEILAMHDWDVVLGSVHWVKGDWIDAPHSAARHEREGTEVLWEEYYRLLAKAAA
                     TGLFDVLTHFDLPKKFGHRRPAALAAAEAAAVAAARE"
     gene            632..1225
                     /locus_tag="PLB01_12870"
     CDS             632..1225
                     /locus_tag="PLB01_12870"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQQ78239.1"
                     /translation="MAIRINRVYTRGGDAGETSLVGGPRIAKDAIRIQSYGTVDELNA
                     ILGLVRTANRETPGASDEDRARLDGLLTRLQNELFNLGSDLATLPQDRHPRQPVIEER
                     HVTFLEKTIDEMNDGLPELRSFVLPGGGWVSAFLHQARTVCRRAERLVTTLARQESVG
                     PHALVYLNRLSDLLFVLGRWAARARREPEPLWEPEQG"
     gene            1222..2775
                     /locus_tag="PLB01_12875"
     CDS             1222..2775
                     /locus_tag="PLB01_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78240.1"
                     /translation="MTRVRVPDLIGLLAALAACPAVAQGPPAISAVEIRTIDVFNDAE
                     AAKFFLYRWANALHVKTKASFVSTQLLFEPGDPLRPEILAETERNLRAFGLFRRVSVK
                     AEGTSVVVETADAWTLLLRGSFSNKGGVTEYSIGAEEYNLLGLGMQFGFGFEQEVTRT
                     SRSVFFADPNLIWPHAAFRIDVSDLSDGQRFSIAYGRPFYALGVPWALEGLVNRADFD
                     TKLYAGGEEAATWRKRQRIARVQGGTRLASGAWGADRLLGSIEWSEVRLQDKDGPPPP
                     DGPASNRRFLFFSGGFQHVGSEWITRRQVDRLERDEDFNLATGLRAEAGISPGFDKQS
                     AAGRLLASVQSGTSFPAGFATFTSTASTRLQSGFQNALVGADARGYVLKPPWAFVGRV
                     GTLVGWRLDPEDQIVLDGATGVRGYRLHAVAGDRRLVANAEARVLLVPEIWSLLSFGL
                     AAFGDAGYSWGAPDGFWHLADAGVGLRVGLTRASKSNLIRIDVARVLHPDPLGRTGWL
                     VSFASGQAF"
     gene            complement(2800..3747)
                     /gene="trxB"
                     /locus_tag="PLB01_12880"
     CDS             complement(2800..3747)
                     /gene="trxB"
                     /locus_tag="PLB01_12880"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQQ78241.1"
                     /translation="MPQKQHYRVVIIGSGPAGLTAAIYAARANLAPLVLEGIQPGGQL
                     TTTTEVENFPGFEHGIQGPELMDVTRKQAERFGTEIAADTVVDVDVKAKPFALTLETA
                     GRITADAVIIASGASAKYLGLESEKKLLGYGVSACATCDGFFFRGKEVVVVGGGDTAM
                     EEATYLTKFCTKVHLVHRRSTFRASAIMAQRALANPKIEVHLDTVVEEVLGEPGTGVS
                     GVRVKNAKTGETKTLAVSGYFAAIGHQPNTAIFAGKLDMNEVGYLKIAHPTTYTSVEG
                     IFAAGDVADFMYRQAISAAGEGCKAAIDAERWLGAQGIH"
     gene            complement(3767..4357)
                     /locus_tag="PLB01_12885"
     CDS             complement(3767..4357)
                     /locus_tag="PLB01_12885"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQQ78242.1"
                     /translation="MPQIVGRQAPDFALEGVLNGRFAHYRLSDYKGKWVVLFFYPLDF
                     TFVCPTEILAFSDRLGEFKKLGAEVLGASVDSKYSHLAWTEKPREQGGIEGLAYPLLE
                     DLGKELAEEYGVLDETGKVALRGLFLIDPDGVVQHATVNNNSVGRSVDETLRVLQAFQ
                     FVRENGEVCPADWKPGTKAMTADWEKSKEYFSTAAK"
     gene            4517..4936
                     /locus_tag="PLB01_12890"
     CDS             4517..4936
                     /locus_tag="PLB01_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQQ78243.1"
                     /translation="MAELKGTKTHENLKHAFAGESQANRRYLYFADKADIEGYPEIAG
                     NFRDTAEGETGHAHGHMDYLKAAGDPATGLPFGSTELNLKSAIEGETYEYTQMYPGFA
                     KTAREEGFAEVADWFETLARAEKSHANRFSKLLTTIG"
     gene            5146..6426
                     /locus_tag="PLB01_12895"
     CDS             5146..6426
                     /locus_tag="PLB01_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HQQ78244.1"
                     /translation="MGAVGTPIAVKDAATLTAELTRIADICHGCRRCFSLCPSFEVLF
                     KGLDVPEVDGEADKLPRKILEDFVDLCYECKLCLPHCPYIPPHRWAVDIPHLVLDARR
                     VRMEAQGLPLRERLLSDPDALGRMASTVAPVANFMNTLPLARWGMEKTLGVAAKKDLP
                     RFVWQTFTRWFETREAPRRVAPPVAKVVLFPTCSVEWNRPEIGRAAVSVLEKNGVEVA
                     VAYPQCCGMPLFDVGDVAGADEARRTFVGAMKGWVEKGYTIVTPGPSCSLMVKKEYPW
                     LAARGGAPDEATALVAKNTRDLFEFLAELKAQGALSLDFPKAPKSIAYHLPCHLKVQN
                     MGFKSRDVLAATGAAVTMVEKCSGHDGTWAMKAEYFDESMKVGQKLFDGLAAAGAEVV
                     ASDCALAGVQIHQGMGVDVLHPIEVLRDAYGLPR"
     gene            6423..7025
                     /locus_tag="PLB01_12900"
     CDS             6423..7025
                     /locus_tag="PLB01_12900"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HQQ78245.1"
                     /translation="MNKLTLADVKNLHDYELIRDDWKRDVIAAKARRRVALGGWISLV
                     FENRLTVLHQVQEMCRAERIVKPAAVQQELDVYNELLPGPGEIAATLLVEITDVERVK
                     DELDKLLGLTSGEHLWLEIAGRRVFARFLDGQSREDRIAAVQYLRFPVGTEPAVRAAL
                     EEGPLPVVLHLAHPHVRASALLSPETRRELARDLAPDGGQ"
     gene            7035..7526
                     /gene="sixA"
                     /locus_tag="PLB01_12905"
     CDS             7035..7526
                     /gene="sixA"
                     /locus_tag="PLB01_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HQQ78246.1"
                     /translation="MILHLLRHAEAEDLSPSGRDADRRLTEDGRRRMKSVAKAVARMD
                     PGYDAILVSPLLRARQTAEPVAEACGFRKPLVETRALEPNADPVDVLHELARLKPGTV
                     LLVGHQPHLGRLFGLLLSGRSRLEVPMKKAGLAAFEAASDPSLEPAELKFYLAPKLLE
                     MLS"
     gene            7606..8205
                     /locus_tag="PLB01_12910"
     CDS             7606..8205
                     /locus_tag="PLB01_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQQ78247.1"
                     /translation="MGKSVALGCIVLILVGVLVVGGMAAGAYNKLNAADQAVSGAWAQ
                     VENVYQRRADLIPNLVETVKGAANFEKETLTQVIQARASATQVKLDANSIKDPAAFEK
                     FQRAQDGLGSAIGRLLVSVERYPELKANQNFRDLQTQLEGTENRISVERGRFNDATQA
                     YNTLRRSFPTVIVANFAGFKERPFFKAAEGADKPPAVKF"
BASE COUNT         1281 a         2817 c         2820 g         1311 t
ORIGIN      
        1 cctcccgcgc ggcggcgacg gcggcggcct cggccgcggc gagcgccgcc ggccgccggt
       61 ggccgaactt cttcgggagg tcgaagtggg tcaggacgtc gaagaggccc gtcgccgcgg
      121 ccttcgcgag gaggcggtag tactcctccc agaggacctc ggtgccctcg cgctcgtgtc
      181 gcgcggcgga atgcggcgcg tcgatccagt cgcccttgac ccagtgcacg cttccgagga
      241 cgacgtccca gtcgtgcatg gcgagaatct ccgcgaggac ggcctcgtgg ccttcggcgt
      301 aatccgcctc gaggccgagg aggacgtcga tccgccccgc gaactcctcg gagagggcct
      361 ggacctcctc gacgtacccc ggcagctcgg cgcgcgtcat cgcgatcccg gggtccgggt
      421 cgtccccggg caggaagtag agcgggacat ggtccgtgaa cgcgatctcg gagaggcccc
      481 gggcgagcgc cgtctcgacg tacgcacgca tcgcgcctcc cgcatggccg cagcgggccg
      541 tgtggacgtg gtagtcggtg ctggtccgga ggttgcatct gggggtcggc atggaacttc
      601 gcggagtgta gtctcggggt tcggaggagt catggcgatc cggatcaacc gtgtctacac
      661 gcgcgggggc gacgcgggcg agacgagcct cgtggggggg ccgcgcatcg cgaaggatgc
      721 gatccgcatc cagagctacg gaaccgtcga cgagctgaat gcgatcctcg gtctcgtgcg
      781 cacggcgaac cgcgagacgc ccggagcgtc ggacgaggat cgtgcgcgcc tggacggact
      841 tctcacgcgc cttcagaacg agctcttcaa cctcggctcg gacctcgcga cgcttccgca
      901 ggaccgtcac ccgaggcagc cggtgatcga ggagcgtcac gtgactttcc tcgagaagac
      961 gatcgacgag atgaacgacg ggctccccga gctccgctcc ttcgtccttc cgggcggcgg
     1021 ctgggtctcg gcgttcctcc accaggcccg gacggtctgc cgacgcgccg agcgcctcgt
     1081 cacgacgctc gcgcggcagg aatccgtcgg tccgcacgcg ctcgtctacc tgaaccggtt
     1141 gtcggacctc ctgttcgtgc tcggccgctg ggccgcgcgc gcgcgccgcg agcccgaacc
     1201 gctctgggag ccggagcagg gttgacgcgc gttcgagtcc cggacctcat cggacttctc
     1261 gcggcgctgg cggcgtgccc cgccgtggct cagggcccgc ccgcgatctc ggcggtcgag
     1321 atccggacga tcgacgtctt caacgacgcg gaggcggcga agttcttcct ctaccgctgg
     1381 gcgaacgccc tccacgtcaa gacgaaggcc tctttcgtga gcacgcagct ccttttcgag
     1441 cccggcgacc cgctgcgccc cgagatcctc gcggagaccg agcgcaacct ccgggcgttc
     1501 ggtctctttc gccgggtctc cgtgaaggcc gagggaacga gtgttgtcgt cgagacggcc
     1561 gacgcctgga cgctcctcct cagggggtcg ttctcgaaca agggcggcgt gacggagtat
     1621 tcgatcgggg ccgaggagta caacctgctc ggactcggga tgcagttcgg cttcgggttc
     1681 gagcaggagg tgacgcggac gtcgcggtcc gtcttcttcg cggatccgaa cctcatctgg
     1741 ccgcacgcgg cgtttcgcat cgacgtgtcc gacctgtccg acgggcagcg cttctcgatc
     1801 gcctacggcc ggccgttcta cgcgctcggc gtgccgtggg cgctcgaggg gctcgtgaac
     1861 cgcgccgact tcgacacgaa gctgtacgcc ggcggtgagg aagccgccac ctggaggaaa
     1921 cggcagagaa tcgcgcgcgt ccagggcggg acccgcctcg cgagcggcgc ctggggcgcg
     1981 gaccgccttc tcggctccat cgagtggtcg gaggtccggc tgcaggacaa ggacgggccg
     2041 ccgccgccgg acgggcccgc gtcgaaccgg cggtttctct tcttttcggg gggattccag
     2101 cacgtcgggt ccgagtggat cacgcgccgg caggtcgaca ggctcgagcg cgacgaggac
     2161 ttcaacctcg cgacgggcct gcgcgccgag gcgggcattt cgccgggctt cgacaagcag
     2221 agcgcggccg gccgcctcct cgcgagcgtc cagtcgggca cctcgttccc ggcaggcttc
     2281 gcgaccttca cgtccacggc gtccacccgc ctccagagcg ggttccagaa cgcgctcgtc
     2341 ggcgccgacg ctcgcgggta cgtgctcaag ccgccgtggg cgttcgtcgg gcgcgtcggg
     2401 accctcgtcg gctggcgtct ggatcccgag gaccagatcg ttctggacgg cgcgaccggc
     2461 gtgcgcggtt accgcctgca cgcggttgcg ggagaccgcc ggctcgtcgc gaacgcggag
     2521 gcgcgcgttc tcctggtgcc cgagatctgg tcgctcctgt cgttcggcct cgcggccttc
     2581 ggggacgcgg ggtactcgtg gggtgcgccg gacggtttct ggcacctcgc cgacgccgga
     2641 gtcgggctgc gggtcgggct cacgcgcgcg agcaagagca acctcatccg gatcgacgtc
     2701 gcacgcgttc ttcacccgga cccgctcggg cgcacgggct ggctcgtctc cttcgcctcg
     2761 ggccaggcgt tctgaggcgc cgaaaagcgc ccgccggcgt cagtggatgc cctgggcccc
     2821 gagccagcgc tcggcgtcga tcgcggcctt gcagccctct cccgccgccg agatcgcctg
     2881 ccggtacatg aagtccgcga cgtcacccgc ggcgaagatg ccctccacgg acgtgtacgt
     2941 cgtcgggtgc gcgatcttca ggtagccgac ctcgttcatg tcgagcttgc ccgcgaagat
     3001 cgccgtgttc ggctggtggc cgatcgcggc gaagtagccg gagacggcga gggtcttcgt
     3061 ctcgcccgtc ttcgcgttct tcacgcgaac gccggatacc ccggtgcctg gctcgccgag
     3121 cacctcctcg acgaccgtgt cgaggtggac ctcgatcttc gggttcgcga gggcgcgctg
     3181 cgccatgatc gcggaagccc ggaacgtgct ccgcctgtgg acgaggtgga ccttcgtgca
     3241 gaacttcgtg aggtacgtcg cctcttccat cgcggtgtcg ccgccgccca cgacgacgac
     3301 ctccttgccg cggaagaaga agccgtcgca cgtggcgcag gccgagacgc cgtagccgag
     3361 gagcttcttc tcgctctcga gcccgaggta cttcgcggag gcgccggagg cgatgatgac
     3421 ggcgtcggcc gtgatgcggc ccgccgtctc cagcgtgagg gcgaacggtt ttgccttcac
     3481 gtcgacgtcg acgacggtgt cggccgcgat ctcggtgccg aagcgttcgg cctgtttgcg
     3541 cgtgacgtcc atgagctccg ggccctggat gccatgctcg aagccgggaa agttctcgac
     3601 ctcggtcgtc gtcgtgagct gcccgccggg ctggatgccc tcgagaacga ggggcgcgag
     3661 gttcgcacgg gccgcataga tggccgcggt gaggccggcg ggaccggacc cgatgatcac
     3721 gacccggtag tgctgcttct gaggcatggg ccgattcttt caggtcttac ttcgccgccg
     3781 tcgagaaata ctccttcgat ttttcccagt cggccgtcat cgccttcgtc ccgggcttcc
     3841 agtccgcggg gcagacctcg ccgttctcgc gcacgaactg gaaggcctgc aggacgcgga
     3901 gcgtctcgtc gaccgagcgg cccacggagt tgttgttgac cgtggcgtgc tggacgacgc
     3961 cgtccgggtc gatgaggaac aggccgcgca gagcgacctt tccggtctcg tcgaggacgc
     4021 cgtactcctc ggccagctcc ttgccgaggt cctcgaggag cgggtaggcg aggccctcga
     4081 tgccgccctg ttcgcgcggc ttttcggtcc aggcgaggtg gctgtacttc gagtcgacgg
     4141 acgcgccgag gacctcggct ccgagcttct tgaactctcc gaggcgatcc gagaacgcga
     4201 ggatctcggt cgggcagacg aacgtgaaat cgagcgggta gaagaacagg acgacccact
     4261 ttcccttgta gtccgagagg cggtagtggg cgaagcgtcc gttcaggacg ccttcgagcg
     4321 cgaagtcggg ggcctggcgg ccgacgatct ggggcatggg agttctcctt gcggtctcgt
     4381 gcggggtctc tcggggcggg accgccccgg cacttgtagt ttagacaaag tccagataaa
     4441 gtccagccct catcgttgca cgaaaggtcc ccgggaccga gattccacgg ggacacagaa
     4501 tgaagaagga ggccacatgg cagaactcaa gggaacgaag acgcacgaga acctcaagca
     4561 cgcgttcgcg ggcgagagcc aggcgaaccg gcgctacctc tacttcgccg acaaggccga
     4621 catcgagggc taccccgaga tcgccggcaa cttccgcgac acggccgagg gcgagacggg
     4681 ccacgcccac gggcacatgg actacctcaa ggccgcgggc gacccggcca cgggccttcc
     4741 gttcggctcg acggagctga atctcaagag cgcgatcgag ggcgagactt acgagtacac
     4801 gcagatgtac ccgggcttcg ccaagaccgc ccgcgaggaa ggcttcgccg aggtcgccga
     4861 ctggttcgag acgctcgcgc gcgccgagaa gtcgcacgcg aaccgcttca gcaagctcct
     4921 gacgacgatc ggctgaagag gagagaaggt ccaggcgccc tgaagcgcgc gaactatggc
     4981 gcggcaggtc ggcagggcgc attttcttag aaggtgaatg gggtgcgagg cgctcgatcg
     5041 cggctgtggc ggcgaggaat gctccgcccc cactcaccct tctaagaaat cttcgtcttc
     5101 ctcttcgcct tctcttcctc cgaatcttcc gaaagcagag tgaacatggg cgccgttgga
     5161 actcccatcg ccgtgaaaga cgcggcgacc cttaccgccg agctcacccg catcgccgac
     5221 atttgccacg gctgccggag gtgcttctcg ctctgtccct cgttcgaggt cctgttcaag
     5281 ggcctcgacg tccccgaggt ggacggcgaa gcggacaagc tcccgaggaa gatcctcgag
     5341 gacttcgtcg acctctgcta cgagtgcaag ctctgcctgc ctcactgccc gtacatcccg
     5401 cctcaccggt gggccgtcga cattccgcac ctggtgctcg acgcgcggcg cgtgcgcatg
     5461 gaggcgcagg gccttccgct ccgcgagcgg ctcctttcgg accccgacgc gctcggccgg
     5521 atggcgtcga cggtcgcgcc ggtcgcgaat ttcatgaaca cgctccctct cgcgcgatgg
     5581 gggatggaga agacgctcgg cgtcgccgcg aagaaggacc tgccgcgctt cgtctggcag
     5641 acgttcaccc gctggttcga gacgcgcgag gcgccgaggc gcgtggcgcc gcccgtcgcg
     5701 aaggtcgtcc tcttcccgac gtgctcggtc gagtggaacc gcccggagat cgggcgggcc
     5761 gccgtctccg tgctcgagaa gaacggcgtc gaggtcgcgg tcgcgtaccc gcagtgctgc
     5821 ggcatgccgc tcttcgacgt cggcgacgtc gcgggcgcgg acgaggcgcg ccggaccttc
     5881 gtcggggcga tgaagggctg ggtcgagaag ggctacacga tcgtgacgcc gggcccgtcc
     5941 tgctcgctca tggtcaagaa ggaatacccc tggctcgccg cacgtggcgg ggccccggac
     6001 gaggccacgg cactcgtcgc gaagaacacg cgggacctct tcgagttcct cgcggagctc
     6061 aaggcacagg gagcgctttc cctcgacttc ccgaaggcgc caaagtcgat cgcctaccac
     6121 ctcccgtgtc acctgaaggt ccagaacatg ggtttcaagt cgcgcgacgt cctcgcggct
     6181 accggcgcgg cggtcacgat ggtcgagaag tgctcgggcc acgacgggac gtgggcgatg
     6241 aaggccgaat atttcgacga gtccatgaag gtcggccaga agctcttcga cggactcgcc
     6301 gccgccggcg cggaggtcgt cgcatccgac tgcgccctcg caggcgtgca gatccaccag
     6361 gggatgggcg tcgacgtcct ccatcccatc gaggtcctcc gggacgcgta cgggctcccg
     6421 cgatgaacaa gctcacgctc gccgacgtca agaacctcca cgactacgag ctcatccgcg
     6481 acgactggaa gcgggacgtc atcgccgcca aggcgcggcg ccgcgtcgcg ctcggcggct
     6541 ggatctccct cgtcttcgag aaccgcctga cggtcctgca ccaggtccag gaaatgtgcc
     6601 gcgccgagcg catcgtgaag cccgcggccg tccagcagga actggacgtc tacaacgagc
     6661 ttctgccggg gccgggcgaa atcgccgcca cgctgctcgt cgagatcacg gacgtcgagc
     6721 gcgtcaagga cgagctcgac aagctcctcg ggctcacctc gggcgagcac ctctggctcg
     6781 agatcgccgg ccgccgcgtc ttcgcgcgct tcctcgacgg gcagagccgc gaggaccgca
     6841 tcgcggcggt ccagtacctg cgtttcccgg tcgggaccga gccggccgta agggccgctc
     6901 tcgaggaggg cccgctcccg gtcgtcctgc acctcgcgca ccctcacgtc cgcgcgagcg
     6961 cgctgctctc gccggagacg cggcgcgaac tcgcgcgcga tctcgcaccc gacggcgggc
     7021 aatagaatcc cgccgtgatc ctccacctcc tgcgccacgc cgaggccgag gacctgtcgc
     7081 cgtcgggccg ggacgcggac cgccggctca ccgaggacgg ccgccggcgg atgaagtccg
     7141 tcgccaaggc ggtcgcccgg atggacccgg gctacgacgc gatcctcgtc tcgccgcttc
     7201 tgcgggcgcg ccagaccgcc gagcccgtgg ccgaggcctg cggcttccga aagcccctcg
     7261 tcgaaacgag agccctcgag ccgaacgccg accccgtgga cgtcctccac gagttggccc
     7321 gcctgaagcc cgggacggtc cttctcgtgg gtcaccagcc gcacctcggg cgcctcttcg
     7381 gactcctcct ttcgggccgg tcccgcctcg aggtcccgat gaagaaggcc ggcctcgcgg
     7441 ctttcgaggc ggcatccgat ccttccctcg agcccgccga gctgaagttc tatctggccc
     7501 cgaagctgct cgaaatgctt tcctgacaga cggttacgtc gactcgaacg gagcctcgaa
     7561 ggtttcctga tagcatgtcc ggctcttgga ggaacggttt cgaatatggg taaaagcgta
     7621 gctctcggct gcatcgtcct catcctcgtc ggcgtgctcg tcgtgggggg tatggccgcg
     7681 ggggcgtaca acaaactcaa cgcggcagac caggccgtca gcggcgcctg ggcccaggtc
     7741 gaaaacgtct atcagcgccg ggccgacctg atcccgaacc tcgtcgagac ggtcaagggc
     7801 gccgcgaact tcgagaagga aaccctcacg caggtcatcc aggcccgcgc gtcggcgacg
     7861 caggtcaagc tggacgccaa ctcgatcaag gacccggcgg ctttcgagaa gttccagagg
     7921 gcgcaggacg ggctcggcag cgcgatcggc cgcctgctcg tcagcgtcga gaggtacccc
     7981 gagctgaagg cgaaccagaa cttccgcgat ctgcagacgc agctcgaggg caccgagaac
     8041 cggatctcgg tcgagcgcgg ccgcttcaat gacgcgacgc aggcctacaa cacgctgcgc
     8101 cgctcgttcc cgaccgtgat cgtcgcgaac ttcgccggct tcaaggagcg cccgttcttc
     8161 aaggccgccg agggcgccga caagccgccc gccgtgaagt tctgaagaga gaacgcagaa
     8221 acgaagaga
//
LOCUS       DAOMFY010000165         8221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2706_length_8221_cov_6.930688, whole genome shotgun sequence.
ACCESSION   DAOMFY010000165 DAOMFY010000000
VERSION     DAOMFY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_8221_cov_6.930688"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PLB01_12915"
     CDS             complement(<1..1534)
                     /locus_tag="PLB01_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78248.1"
                     /translation="MSGFWRAVAAQAAVETRMRLRAPATLVAVLLLGAASVFWIPDPS
                     GRATSLAWKMADGRDLAPEYSSGYLAWAASIVASLWASLVGFYLVAGSIRRDRERGVG
                     AILAATPLPDAAYLAGKAAAHVLYLGVLSLLAVGAALVAFLRWGVGAFHPLDFAATWA
                     LFILPALLLTASVAVLFDVAPVLRSRAGYVIWFFAFAILVSGVNTRDGRGRVVRAPRV
                     DPLGMATLDWRVSKSMPGAVSVSSGLIFHDKPFLRVPWPGVKQDPAETAWRLVSLVAA
                     LLPLGASLFFLDRFAPARSGRRRGRESPAPAAAAAEDASPVAALATFEAPDPSALRAV
                     LAEARLTWDSGSLLKWPLLAAALAAPFLPGAAFPLGAAGMLLLLALAVSEVAGREDLA
                     GTRGLVFAQPGVPASPVLWKTGANVVFVLAFGLPCAVRAAAESPAAGAAFLSGLVFTA
                     GLAAGFGSLTGGGKLFLGVYTAVWYFAVNRLPISDFTGLFAEPSALKAAAFALAGAAA
                     VGG"
     gene            complement(1531..2406)
                     /locus_tag="PLB01_12920"
     CDS             complement(1531..2406)
                     /locus_tag="PLB01_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78249.1"
                     /translation="MLEVRGLSKTYRSGPAALSDVSFDMEEGVLGLLGPNGAGKTTLL
                     SILATVTKPSAGTFRWQGRDAVRDPFAARRVLGYLPQDFGVYERLTAREFLLYLGRLK
                     GLSGAALASRVGDLLTLVNLHGAADRRLGGFSGGMRQRVGIAQALLGDPKLLIVDEPT
                     VGLDPEERVRFRNLLAEVAHGRVVILSTHIVSDVEAVASKIAMLSGGRLRGIATPEEL
                     LGRAAGRVFTAVVPAASVASVQARVPVSGMVRRADGVHVRYVGSHSDVDAARPVEPTL
                     EDAYLLSSLEGARAA"
     gene            complement(2455..4059)
                     /locus_tag="PLB01_12925"
     CDS             complement(2455..4059)
                     /locus_tag="PLB01_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQQ78250.1"
                     /translation="MARDFDTDFAVVGSGFGGSVSALRLAEKGWRVVVLEKGQRFAPE
                     DFPKTNWNLPKWLWLPGLGFRGIFKMTFLKHVTALSGVGVGGGSLVYANTLPVPADEF
                     FGLPSWGSLADWKAELAPHYATAKRMLGTARVPFTALQDRVLKQVGEDIGKGDSWHPT
                     DVAVFFGEPGVTVPDPYFGGEGPDRTGCKSTGACMIGCRTGAKNTLDKNYLWLAEKRG
                     ARIESDAEVTWVRPLEGGGYELTVRRGREPLPFLRKKTTLTARHVVFAGGVIGTVELL
                     LKLKESPEGLPKLSDRLGEFVRTNSEVLMGVVSPKDDLSKGVAIGSILETDEHSHLEP
                     VHYPAGSGFFRLLAMPHAPGATVAERFASALGVLFRQPGKTLKAWATPDWAKHTMILL
                     YMRTADGTLRMKLGRGLRNGFRHGLATDAGTGPQAKAWLPEATELARRVEEKLGGTAQ
                     SLLTETLLGIPTTAHILGGCCMGETPAGGVIDAKNRVFGYDGLWVVDGSAISANPGVN
                     PSLTITALAERAMTFVPENAAATRAA"
     gene            complement(4069..4755)
                     /locus_tag="PLB01_12930"
     CDS             complement(4069..4755)
                     /locus_tag="PLB01_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78251.1"
                     /translation="MPLALHPRVRALTAGERAAVLGAALLVFAFAAGPVWRHRWEPNA
                     AILLSYAVIPPGVFAAFFRRGTPRLVPVLVESLLLSIAKFGISAFVLVTLWSFGTPPP
                     AARVGGLLEDVLRPKSTRISSSERERAAAGGARRLAAAMDVSVGDGGFSSKVISLAAG
                     GDVAFRAADGRLHTLELLSADGSIGANVPVLASGAPRLVTFEEISLFSSIRCAVHPEE
                     RADLTGVPGR"
     gene            4812..5261
                     /locus_tag="PLB01_12935"
     CDS             4812..5261
                     /locus_tag="PLB01_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78252.1"
                     /translation="MGRILIVAAAVVFAIWYFRHKPAEPVTTSESSPAVSAGPGCIQE
                     AENASRLLADASRLLANMPVDANAWSDAENRTTQAISRAESACSGGSTEAERSALADA
                     REALSLIRTSLSEAAGAAKGSGGFQGALRQEQIDSRLSSARAKLGLR"
     gene            complement(5268..6425)
                     /locus_tag="PLB01_12940"
     CDS             complement(5268..6425)
                     /locus_tag="PLB01_12940"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQQ78253.1"
                     /translation="MTFSRLSHLACTKCGATYDVGPLLNVCTAPGCAGSLFAAYDLAP
                     FRRDDLDARPPGMWRWHEVMPARSAGEIVTLGEGGTPLLRADRLGAAHRLANLWVKEE
                     AGNPTGSFKARGLGAAVTMAKALGAKKIALPTAGNAGGAAAAYAAKAGLECHVFMPKD
                     TPKVFRIECEAYGAKVDLVDGLIDDCGRIVGERKEAEGWFDVSTLKEPYRVEGKKTMG
                     YEIAEQLGWTLPDVVVYPTGGGTGLIGMWKAFDEMEALGWIGPERPRMVSVQAEGCAP
                     IPKALAEGKDVSVRFENAHTYASGLRVPKAYADYLILKILRASKGEAVTVTDDEMRRG
                     VLELASREGLFAAPEGGAAWEAVKKLVAEGKVDRGERIVVFDTGTGYKYVE"
     gene            complement(6430..6780)
                     /locus_tag="PLB01_12945"
     CDS             complement(6430..6780)
                     /locus_tag="PLB01_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78254.1"
                     /translation="MKRSLAVLALSLAGCASTSRDMSGPVVGVPLDTPDHFMVARSGI
                     GKAEEPKADGVCRNPMVDPRDKTELLLRRSAGGKGDYGVDAGVFGGGKYGMTTQQLLR
                     IDCATGKAIGIVAQ"
     gene            complement(6789..7823)
                     /locus_tag="PLB01_12950"
     CDS             complement(6789..7823)
                     /locus_tag="PLB01_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQQ78255.1"
                     /translation="MPRSARSVLRAEIGEQPHAVARVLAREGRRTASLAARLAKADLS
                     WILVAARGTSDNAARFAQYAFGIVHRLPVALAAPSLVTLYGRPPRVKGALVIGISQSG
                     RSPDVVETVAAARRAGAPTLAIVNDPASPLARAAEWVLPLHAGVERSVAATKTYTAQL
                     AAVALLVLSMAGRKKDLAALAAIPGRMAEALGVEPEAEAAAGSLARAQRAVVIGRGLH
                     YPTACEIALKLKELALLLAEPYSAADFQHGPIAMAERDLPVVLVSPPGSRPAHEVEAL
                     GRELRRRGSPVLAIGPPRGSALGVPRVPELLAPLVSILPGQLLAFHAARARGLDPDRP
                     RGIHKVTETR"
     gene            7851..>8221
                     /locus_tag="PLB01_12955"
     CDS             7851..>8221
                     /locus_tag="PLB01_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78256.1"
                     /translation="MFGRERKTPRRRTPRFLRPVLRPMAFRVLPRELGPRRVGRLAAV
                     LPEGVRDPGFEVLLRRIDASPIHRGNTVALYFDGSKAFAAMCAAIGEAREEVLLEAYI
                     LKDDAAGRALAGALAGAARRGV"
BASE COUNT         1248 a         2971 c         2985 g         1017 t
ORIGIN      
        1 cgccccccac cgccgcggcg cccgcgaggg cgaacgcggc ggccttcagg gccgacggct
       61 ccgcgaagag gcccgtgaag tccgagatcg ggagacggtt cacggcgaag taccagacgg
      121 ccgtgtagac gccgaggaat agcttgcccc cgccggtgag ggagccgaat cccgcggcga
      181 ggcccgccgt gaagacgagg cccgacagga acgcggctcc cgcggccggg ctctcggccg
      241 cggcgcggac cgcgcacggc aggccgaacg cgaggacgaa gaccacgttc gcgcccgtct
      301 tccacaggac cggcgacgcc ggaacgcccg gctgcgcgaa gacgaggccg cgcgttccgg
      361 cgaggtcctc gcgccctgcg acttcggaca cggcgagcgc gaggagcagg agcatcccgg
      421 ccgcaccgag cgggaaagcc gcgccgggca ggaagggagc cgcgagcgcg gcggccagga
      481 gcggccactt caggagcgag cccgaatccc acgtgagccg cgcctccgcg aggacggcgc
      541 gcagcgcaga ggggtcgggc gcctcgaatg tggccagcgc ggccaccggg gacgcgtcct
      601 cggccgccgc cgcggccggc gcaggactct cgcgtccgcg cctccggccc gagcgcgcgg
      661 gggcgaagcg gtcgaggaag aaaagggagg cgccgagcgg gaggagcgcg gccacgaggg
      721 acacgaggcg ccaggccgtc tcggccggat cctgcttcac gccgggccag gggacgcgga
      781 ggaacggctt gtcgtgaaaa atgaggccgc tcgaaaccga gacggcgccc ggcatcgact
      841 tcgagacgcg ccagtcgagc gtcgccatcc cgagcgggtc gacgcgcggc gcgcgcacga
      901 ctcggcccct gccgtcgcgc gtgttcaccc cgctcacgag gatcgcgaac gcgaagaacc
      961 agatcacgta gcccgcgcga gagcgcagga cgggcgcgac gtcgaacagg accgcaaccg
     1021 acgcggtgag gaggagcgcc gggaggatga acagcgccca ggtcgccgcg aagtccagcg
     1081 ggtgaaacgc cccgacgccc cacctcagga acgcgacgag agcggcgccg acggcgagaa
     1141 gcgacaggac gccgaggtag aggacgtgcg cggcggcctt tcccgcaagg taggcggcgt
     1201 ccggaagcgg cgtcgcggcg aggatcgcgc cgacgccgcg ctctcggtcg cgacggatcg
     1261 agccggcgac gaggtagaac cctacgagcg acgcccagag gctcgccacg atcgaggcgg
     1321 cccatgcgag gtagcccgag gagtactcgg gggcgaggtc ccggccgtcc gccatcttcc
     1381 acgcgagcga cgtggcgcgg cccgacggat ccgggatcca gaagacggaa gcggcgccga
     1441 ggaggaggac ggcgacgagc gtcgcgggcg cgcgcaggcg catccgcgtc tcgacggcgg
     1501 cctgtgccgc gaccgcacgc cagaacccgc tcacgccgct cgcgccccct ccaggctcga
     1561 aaggagatag gcgtcctcga gcgtgggctc gacgggccgc gcggcgtcga cgtccgaatg
     1621 actcccgacg taccgcacgt gaactccgtc agcgcggcgg accatgcccg agaccggtac
     1681 gcgggcctgg acggacgcga cggacgcggc cgggacgacg gccgtgaaga cgcggcccgc
     1741 cgcgcgcccg aggagctcct cgggcgtcgc gatcccgcgc agccgcccgc ccgagagcat
     1801 cgcgatcttc gaggcgaccg cttcgacgtc cgaaacgatg tgcgtcgaga ggatcacgac
     1861 gcgcccgtgg gcgacctccg cgaggaggtt ccggaagcgc acgcgctcct ccggatcgag
     1921 gccgaccgtc ggctcgtcca cgatcaagag cttcgggtcg ccgaggaggg cctgcgcgat
     1981 gccgacgcgc tggcgcattc cgccggagaa gccgccgagc cggcggtccg cggccccgtg
     2041 gaggttcacg agtgtcagga ggtctcccac gcgcgacgcc agcgccgcgc ccgagaggcc
     2101 cttgaggcgt ccgaggtaga ggaggaactc gcgggccgtg aggcgctcgt acacgccgaa
     2161 gtcctgaggg aggtagccga ggacgcgccg cgcggcgaag gggtcccgga ccgcgtcccg
     2221 tccctgccag cggaacgtcc cggcgctcgg cttcgtgacg gtcgcaagga tcgaaagaag
     2281 cgtcgtcttc ccggcgccgt tcgggcctag gaggccgagg acgccctcct ccatgtcgaa
     2341 cgagacgtcc gacagcgccg ccggcccgga gcggtacgtc ttcgaaaggc cgcggacttc
     2401 gagcatggct cccccgccgc tgaaaacgga cgcggggaga agaaggttcc gggctcaggc
     2461 cgcgcgggtc gccgcggcgt tctcgggcac gaacgtcatc gcccgctcgg cgagcgccgt
     2521 gatcgtgagc gaggggttca cgccggggtt tgccgagatc gccgagccgt ccacgaccca
     2581 gaggccgtcg tacccgaaga cgcggttctt cgcgtcgatg acgccgcccg cgggcgtctc
     2641 tcccatgcag cagccgccga ggatgtgcgc cgtcgtcggg atcccgagga gcgtctccgt
     2701 caggaggctc tgcgcggtgc cgccgagctt ctcctcgacg cgccgcgcga gctccgtcgc
     2761 ctcgggcagc cacgctttcg cctgcggccc cgtcccggcg tccgtcgcga gcccgtggcg
     2821 gaatccgttg cgcaggccgc ggccgagctt catccggagc gttccgtccg cggtccgcat
     2881 gtagaggagg atcatcgtgt gcttcgccca gtcgggcgtc gcccacgcct tcagcgtctt
     2941 gcccggctgg cggaagagga cgccgagcgc gctcgcgaag cgctcggcga ccgtggcgcc
     3001 gggcgcgtgc ggcatcgcga gcaggcggaa gaatcccgag cctgcgggat agtgcacggg
     3061 ctcgaggtgc gagtgctcgt cggtctcgag gatcgacccg atcgcgacgc ccttcgagag
     3121 gtcgtccttc gggctcacga cgcccatgag cacctcggag ttcgtccgca cgaactcgcc
     3181 gagccggtcc gagagtttcg gaaggccctc cggactctcc ttgagcttca ggagaagctc
     3241 gacggtcccg atgacgccgc ccgcgaacac gacgtgccgc gccgtcagcg tcgtcttctt
     3301 cctgaggaac gggagcggct cgcggccgcg ccgcacggtc agctcgtagc cgccgccctc
     3361 gagcggcctg acccacgtga cctcggcgtc ggactcgatc cgcgcgcccc tcttctcggc
     3421 gagccagagg tagttcttgt cgagcgtgtt cttggcgccg gtccggcagc cgatcatgca
     3481 ggcgccggtg gacttgcagc ccgtgcggtc gggcccttcc ccgccgaagt acgggtccgg
     3541 gaccgtgacg cccggctcgc cgaagaagac ggcgacgtcc gtcggatgcc aggagtcgcc
     3601 cttcccgatg tcctcgccga cctgcttcag cacccggtcc tggagcgccg tgaacgggac
     3661 gcgcgccgtg ccgagcatcc gcttcgcggt cgcatagtgg ggcgcgagct cggccttcca
     3721 gtccgcgagg gaaccccagc tcggcaggcc gaagaactcg tcggcgggga cgggcagcgt
     3781 gttcgcgtac acgagcgacc cgccgccgac gccgacgccc gagagcgccg tcacgtgctt
     3841 gaggaacgtc atcttgaaga tgccgcggaa cccgagaccg gggagccaga gccacttcgg
     3901 gaggttccag ttcgtcttcg ggaagtcctc gggcgcgaag cgctgcccct tctcgaggac
     3961 gacgacgcgc cagcccttct cggcgaggcg cagcgccgac acgctcccgc caaagccgga
     4021 gcccacgacg gcaaaatcgg tgtcgaagtc gcgcgccacg tggtgagtct atcgcccggg
     4081 cacgccggtg agatcggctc tctcctccgg gtggacggcg cagcgaatcg aggagaagag
     4141 agagatttcc tcgaaggtca cgaggcgagg cgcccccgat gccagaaccg ggacgttcgc
     4201 gccgatcgac ccgtcggcgg aaagaagctc cagcgtgtgg aggcgaccgt ccgccgcacg
     4261 aaacgcgacg tcgccgcccg ctgcaaggga aatcactttc gaagagaatc ccccgtcccc
     4321 cacgctcacg tccatcgccg ccgccagtcg tcgcgcgccg ccggccgccg cacgctctct
     4381 ttcactcgaa gaaatcctcg tgctcttcgg acgcagcacg tcctcgagaa gaccgcccac
     4441 gcgcgccgcc gggggcggcg tcccgaacga ccacagcgtc acgagaacga acgccgagat
     4501 cccgaacttc gcgatcgaga ggagcagcga ctcgacgagc acgggcacga gacgcggcgt
     4561 tccgcgccgg aagaacgccg cgaagactcc gggcggaatc acggcgtagc tcaggagaat
     4621 cgcggcgttc ggctcccagc ggtgccgcca gacgggaccg gccgcgaacg cgaagacgag
     4681 gagcgcggcc ccgaggaccg ccgcgcgttc tcccgccgtg agagcgcgga cccgcgggtg
     4741 aagggcgagc ggcacggcgc ggattctcgc aggagcgcgt gctaggctgg agacggagac
     4801 cgaggctcat catgggaaga atcctcatcg tcgccgccgc cgtcgtcttc gcgatctggt
     4861 acttccggca caagccggcc gaaccggtca cgacgtccga gtcctccccg gccgtctccg
     4921 cgggcccggg ctgcatccag gaggctgaga acgcgagccg cctcctcgcg gacgcctcgc
     4981 gcctcctcgc gaacatgccg gtggacgcga acgcgtggtc cgacgcggag aaccgcacga
     5041 cgcaggcgat ctcgcgcgcc gagtccgcct gctcgggagg cagcaccgag gccgagcgct
     5101 ccgcgctcgc cgacgcgcgc gaggcgctct ctctgatccg cacctccctc tccgaggccg
     5161 ccggcgccgc gaagggctcc ggcgggttcc agggtgcgct gcgacaggag cagatcgaca
     5221 gccggctctc gtccgcgagg gcgaagctcg gcctgcggta gatcaaccta ctcgacgtac
     5281 ttgtagccgg tccccgtgtc gaagacgacg atccgctcgc cgcgatcgac cttcccttcc
     5341 gcgaccagct ttttgacggc ctcccaggcg gcgccaccct cgggcgcggc gaagaggccc
     5401 tcgcgcgagg cgagctcgag gacgccgcgg cgcatctcgt cgtccgtcac ggtcaccgcc
     5461 tcgcccttcg aggcgcgcag aatcttcagg atcaggtagt ccgcgtacgc cttcgggacg
     5521 cgcaggccgc tcgcgtacgt gtgcgcattc tcgaaacgca cggacacgtc ctttccctcc
     5581 gcgagcgcct tcgggatcgg cgcgcagccc tcggcctgca cggagaccat ccgcgggcgc
     5641 tccgggccga tccatccgag cgcctccatc tcgtcgaacg ccttccacat cccgatgagc
     5701 cccgtgccgc cgcccgtcgg gtagacgacg acgtccggaa gcgtccagcc gagctgctcg
     5761 gcgatctcgt agcccatcgt cttcttgccc tcgacgcggt acggctcttt gagcgtcgag
     5821 acgtcgaacc agccttcggc ctccttgcgc tctccgacga tccttccgca gtcgtcgatg
     5881 aggccgtcca cgagatcgac cttcgcgccg tacgcctcgc actcgatccg gaagaccttc
     5941 ggcgtgtcct tcggcatgaa gacgtggcac tcgaggcccg ccttcgcggc gtacgccgcg
     6001 gcggctccgc ccgcgttgcc cgcggtcggg agcgcgatct tcttcgcgcc gagcgccttg
     6061 gccatcgtga ccgccgcgcc gagcccgcgc gccttgaacg agcccgtcgg gttgccggcc
     6121 tcttccttga cccagagatt cgcgaggcgg tgggcggcgc cgaggcgatc cgcccggagc
     6181 agcggcgtgc cgccttctcc gagcgtcacg atctcgcccg ccgagcgggc cggcatcact
     6241 tcgtgccagc gccacatgcc gggcggccgc gcgtccaggt cgtcgcgccg gaacggcgcg
     6301 aggtcgtatg cggcgaagag cgagcccgca cagcccggag ccgtgcagac gttgaggagc
     6361 ggcccgacgt cgtacgtcgc accgcacttc gtgcaggcga gatgggagag ccgcgagaac
     6421 gtcacgccgt cactgagcga cgatgccgat cgccttgccg gtcgcgcagt cgatccgcag
     6481 gagctgctgc gtcgtcatgc cgtacttccc gccgccgaag actcccgcgt ccaccccgta
     6541 gtcgcccttc ccgccggcgg aacggcgcag gaggagctcg gtcttgtcgc gcgggtcgac
     6601 catggggttg cggcagaccc cgtccgcctt cggctcctcg gccttgccga tccccgagcg
     6661 cgccaccatg aagtggtccg gggtgtcgag cggaacgccg acgaccggcc cggacatgtc
     6721 gcggctcgtg gacgcgcagc cggcgaggga aagagcgaga acggcaagcg acctcttcat
     6781 gacggctcct atcgggtctc ggtgaccttg tgaatgccgc gcgggcggtc cgggtcgagg
     6841 ccgcgggccc gcgccgcgtg gaaggcgaga agctgtccgg ggaggatcga gacgagcggg
     6901 gcgagaagct cgggcacccg gggcacgccg agcgcggagc cgcgcggcgg gccgatcgcg
     6961 aggacggggc tcccgcgccg ccggagctcg cggccgagcg cctccacctc gtgcgcgggg
     7021 cgcgagccgg gcggcgagac gagcacgacg ggcaggtccc gctcggccat cgcgatcggg
     7081 ccgtgctgga agtccgcggc cgagtagggc tcggcgagca ggagcgcgag ctccttgagc
     7141 ttgagcgcga tctcgcaggc cgtcgggtag tgcagcccgc gcccgatcac gaccgcgcgc
     7201 tgcgcgcggg cgagcgatcc ggccgcggcc tcggcctcgg gctcgacgcc gagggcctcg
     7261 gccatgcggc cggggatcgc tgcgagagcc gcgagatcct tcttccggcc tgccatcgac
     7321 aggacgagga gcgccacggc cgcgagctgc gccgtgtagg tcttcgtcgc ggcgacgctg
     7381 cgctcgacgc ccgcgtggag cgggagcacc cactcggccg cgcgcgcaag cgggctcgcc
     7441 gggtcgttca cgatcgcgag cgtcggggcc ccggcgcggc gagccgcggc gacggtctcc
     7501 acgacgtccg gcgaccggcc cgactgcgag atcccgatca cgagagcgcc cttcacgcgc
     7561 ggcggccggc cgtagagcgt gacgagggac ggcgccgcga gtgcgacggg cagccggtgg
     7621 acgatcccga acgcgtactg cgcaaagcgg gccgcgttgt ccgacgtgcc gcgggccgcg
     7681 acgaggatcc acgagaggtc ggccttggcg agacgcgccg cgagcgacgc cgtgcgccgc
     7741 ccctcccgcg cgaggacgcg cgccacggcg tgcggctgct cgccgatctc ggccctgaga
     7801 acggagcgcg cggagcgagg catcgtgcga ggatagcgcg gaggcaggcc gtgttcggac
     7861 gcgagcgcaa gacaccgcgg cgccggacgc cgcgtttcct gaggccggtg ctgcgcccga
     7921 tggcgttccg cgtcctcccc cgcgaactcg ggccccggcg cgtgggccgc ctcgcggccg
     7981 tcctgcccga aggcgtgcgc gatcccgggt tcgaggtcct gctccggagg atcgacgcct
     8041 cgccgatcca ccgtgggaac acggtcgcgc tctacttcga cggctcgaag gcgttcgcgg
     8101 cgatgtgcgc ggcaattgga gaggcacggg aggaagttct cctcgaggct tacatcctca
     8161 aggatgacgc ggccggccgc gcgctcgccg gcgccctcgc gggcgcggcc cgccgcggcg
     8221 t
//
LOCUS       DAOMFY010000166         8220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2707_length_8220_cov_6.635395, whole genome shotgun sequence.
ACCESSION   DAOMFY010000166 DAOMFY010000000
VERSION     DAOMFY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_8220_cov_6.635395"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..171
                     /locus_tag="PLB01_12960"
                     /pseudo
     CDS             67..171
                     /locus_tag="PLB01_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563593.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
     gene            261..998
                     /locus_tag="PLB01_12965"
     CDS             261..998
                     /locus_tag="PLB01_12965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ78257.1"
                     /translation="MPKPTDEESLGRELLQLDWGLGLFRWVHLADALDRSPGATRVLS
                     VGSGGGLHEAFLARTRPGLDVTGVDLRDPWVQRDLPNLRFLTGDLLDPSFRTTLPKAD
                     FVYSIECLEHIEDDTTVARAMADVLAPGGTFYLQLPFASRAEQSDPALCRTERRNHGH
                     VRPGYDAEGLQRLVEGLGLEVERVACAFWFPLQPMVWAALEKFGPILVPRWREVLALV
                     SSDVRDGIAADRTAATAVKILARKPAR"
     gene            complement(967..2310)
                     /locus_tag="PLB01_12970"
     CDS             complement(967..2310)
                     /locus_tag="PLB01_12970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQQ78258.1"
                     /translation="MSHETPDVLREELERTRHELRQARERIAMMEMSFFWKLRNAWWA
                     FKDLFQARDSAASPSTRDRNVAWHMPPRDLPPDPDPTGGRFTGKRVLFLLPIAERGGG
                     ANIVLREALAMRSMGVDARVVNLRSFEDAFRGAYADPKVPVAFASPGGIAEVARGFDA
                     VVATANRSVEWLLPLAGVPGGPVLGYYVQDFEPYFYDEGSAGRAHALASYTLVPGLVR
                     FAKTGWNAKEVEARCGVPCAVVGPSLDTTIFRPLGLRRPDPPPVRISAMIRPSSPRRQ
                     PELTLRVFERLHERFGDSVDFTLFGVDPGDAAFRRMSKAFPHRNLGVASDERLAELFN
                     ETHVFADFSTYQAMGLTAMEAMACGAAVIVPAAGGASSFAADGRNALVVDTARSEACE
                     AALVRLVEDAGLAARLGVAARADMETFRPEGAAARILACLFETAPVSAPASARGS"
     gene            complement(2333..3862)
                     /locus_tag="PLB01_12975"
     CDS             complement(2333..3862)
                     /locus_tag="PLB01_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78259.1"
                     /translation="MSELLDRRPAGALAVLCLAGALPVVRALLVPDLNWCYRYMTSDS
                     YDWISNGLFLAGEPVTSTMRPPGLPLLIAALWKAGALSFLPVVNFLFLGLSTAALFLL
                     LRERHDGWIAALACWFFFANDAVQDLARYVMAETYGTPFLVLAALAFVRAGRDPRAYV
                     AMGAALGAGVLFSYAAALSALGLAVAVLILRPADVRRRSLWWGVLAAGAPLGAWVAFR
                     ASYYRAHPGLLRHGVETLIRPNLSNLSLYAVALPAVLGLLVLPLYVAGAFRLDARDET
                     ARRYRAAVFAPLLALSAFWLFVYDWADRRFLVYLLPFLVCFLAEGLEWMRAFARRGRL
                     ATAGAAAFVVAALLWNQIRYPTYGFYFLALTPEHFLEAAMTQTPAQKTELHLTGARIA
                     RLHGSLRSAFSRGLFDWREKAPECPQTDSSFTCLATLKREADSLLRPGQPVGLFTPRG
                     WPQDYWASTNRLANAWRRPVALPSIAEITLAGEEALPKAEGAHLPVVAHCGPYILVRT
                     R"
     gene            complement(3873..5831)
                     /locus_tag="PLB01_12980"
     CDS             complement(3873..5831)
                     /locus_tag="PLB01_12980"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQQ78260.1"
                     /translation="MTLDAPGGDPGPVWGGTPTGSIFFSTDGGSTFASRFTGLPAAPV
                     TRVVSVTPDGRSAYATFAGYLGLPSKHVFRTTDAGVTWTNISNNLPDVPILDIKVDPT
                     DPTDIFLGSDVGVFRSTNGGASWATFNAGLPNVPVYQLAFHPVTNDLWAATYGRGVWR
                     VTNPVSAAPVANFSFNPASPVAGQTIQFTDASTGSPTSWSWDFGDAGASTVQNATHAF
                     ATAGTFPVKLTVSNSGGSNAITKSVVVAPSGASTCVEDATTMCLVGGRYRIRSHWKNQ
                     YAGGAVATLSKAKLTDSTGAFWLFDGATYEYMIRINTATDNGKAWIAIPTFTQVEFWI
                     DVTDTRTGQSQQYHSPAGNQTLIYDPGTFVYPLVGGDPGGPAPSSLGSEPVREDPSTG
                     SNQSGRQSLAQPGCTICLAGTASVAWNGNSGSFHVDRVVNNRGSGFSGPLSVTVVLAN
                     PLPAYGQTISGAYTFSGALSLSPLQAGYQYSQVNSGSVSFFGSSMPPGEYWQLLFLSE
                     NVSGTWYYADWIVMDKKVSCNGTGCTTINSCVEDANTMCLIGGRYRVTSRWKNQYAGG
                     AAANLYKAKLTDATGAFWLFDGATYEYMIRINTATDNGKAWIAIPTFTSVEFWVTVTD
                     TINGLTKEYHSAPGNVTLLYDPFYFTYP"
     gene            complement(7914..>8220)
                     /locus_tag="PLB01_12985"
     CDS             complement(7914..>8220)
                     /locus_tag="PLB01_12985"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HQQ78261.1"
                     /translation="TAAPSWREAPDEERTDVRVAPPPRPTNRGRIKFRVMPDDAAVYM
                     DDKYLGAADDLAANARGIVAEPGTHTITVTRPGYKTKTVEVTARAGSPVDVVIELEK"
BASE COUNT         1457 a         2687 c         2756 g         1320 t
ORIGIN      
        1 attctattgc gggtcaggtc gataacctat ttctgagagt ggggaacggg atcggggaac
       61 acgccgatga ataaggccac cttcaaggac agcccaccgc tgaccgatcg ggtgaatgcc
      121 tatgatgaag cccatctcgc gacttatctc cggctgctcg acgcggccga gggcgcgctc
      181 gcgctcgcgg gcagcctcgc cgactcccgc cgcaacgtcc gcctcgccac cgaagcctac
      241 gcgctggggc tgtgacggcg atgccgaagc ccaccgacga agagagcctc gggcgcgagc
      301 tgctgcagct cgactggggg ctgggcctct tccgctgggt gcacctcgcg gacgccctcg
      361 accgcagccc cggcgcgacg cgcgtgctct cggtcggaag cggcggcggc ctgcacgagg
      421 cattcctcgc gcgcacgcgc ccgggactcg acgtgaccgg cgtcgacctg cgcgatccct
      481 gggtccagcg cgaccttccg aacctccgct tcctaacggg cgacctgctc gacccgtcgt
      541 tccggacgac gctgccgaag gcggacttcg tctactcgat cgagtgcctc gagcacatcg
      601 aggacgacac gaccgtcgcc cgcgcgatgg ccgacgttct cgccccggga ggaacgttct
      661 accttcagct gccgttcgcc tcccgggcgg agcagtcaga ccctgcgctc tgccggaccg
      721 agcgcaggaa ccacggccac gtgcgccccg gttacgacgc ggagggtctc cagcggctcg
      781 tcgagggcct cggcctcgag gtcgagcgtg tcgcctgcgc cttctggttc ccgctccagc
      841 cgatggtctg ggcggcgctc gagaagttcg gcccgatcct cgtaccgcgc tggcgggagg
      901 tcctcgccct cgtgtcgagc gacgtccgtg acggaatcgc cgcggaccgc acggcggcga
      961 cggccgtcaa gatcctcgcg cggaagccgg cgcgctgacg ggcgccgtct cgaacagaca
     1021 cgcgaggatc ctcgccgccg ccccctcggg ccggaacgtc tccatgtccg cgcgcgcggc
     1081 gacgccgagg cgcgcggcga ggccggcgtc ctcgacgagg cgcacgagcg cggcctcgca
     1141 cgcttcgctc cgggccgtgt cgacgacgag ggcattgcgg ccgtccgcgg cgaagctcga
     1201 cgcgcccccg gccgccggaa cgatcacggc ggcgccgcac gccatcgctt ccatcgccgt
     1261 gagccccatc gcctggtacg tcgagaagtc cgcgaagacg tgcgtttcgt tgaacagctc
     1321 cgcaaggcgc tcgtcgctcg cgacgccgag gttccggtgc ggaaacgcct tcgacatccg
     1381 gcggaacgcg gcgtcgcccg ggtcgacacc gaagagcgtg aaatcgaccg agtccccgaa
     1441 acgttcgtgg aggcgctcga acacgcggag cgtgagctcc ggctggcggc gcggagagct
     1501 ggggcggatc atcgccgaga tccgcacggg cggcggatcc ggtcgccgga ggccgagcgg
     1561 ccggaagatc gtcgtgtcga ggctcggccc gacgacggcg cacggcactc cgcagcgggc
     1621 ctcgacctct ttggcattcc acccggtctt cgcgaaccgg acgagacccg gcacgagcgt
     1681 gtacgaggcg agggcgtgcg cacggcccgc cgagccctcg tcgtagaagt acggctcgaa
     1741 gtcctggacg tagtagccga ggacggggcc gcccgggacg cccgcgagcg gcaggagcca
     1801 ctcgaccgaa cggttcgccg tcgcgacgac ggcgtcgaac ccgcgggcga cctccgcgat
     1861 cccgcccggc gaggcgaacg cgaccggaac cttgggatcg gcgtacgcgc cacgaaaagc
     1921 gtcctcgaac gagcgcaggt tgacgacgcg cgcgtcgact cccatcgacc gcatcgcgag
     1981 ggcctcccga aggacgatgt tcgcgcctcc ccctcgctcg gcgatcggca gaagaaagag
     2041 aacccgcttg ccggtgaagc gcccgccggt cggatccgga tcgggcggca ggtcccgcgg
     2101 aggcatgtgc cacgcgacgt tccggtcccg cgtcgacgga cttgccgcgg agtcccgcgc
     2161 ctggaacagg tccttgaacg cccaccacgc gtttcggagc ttccagaaga agctcatctc
     2221 catcatggcg atgcgctcgc gcgcctgtcg aagctcgtgc cgggttctct cgagctcctc
     2281 gcggagcaca tcgggggtct cgtgactcaa cgtgcttcga ttctagggtt tctcaccgcg
     2341 ttcgcacgag gatgtagggg ccgcagtggg ccacgacggg aaggtgtgcc ccctcggctt
     2401 tgggcaatgc ttcttccccg gcgagcgtga tctcggctat cgacggaagt gccaccggcc
     2461 gcctccaggc gttggcgagc cggttcgtgc tcgcccagta gtcctgcggc cagccgcgag
     2521 gcgtgaagag tcccaccggc tgtcccggcc ggagaaggct gtccgcctcc cgtttcagcg
     2581 tcgcgagaca cgtgaaggac gagtccgtct gcgggcattc cggcgctttc tcgcgccagt
     2641 cgaaaagacc gcgggagaac gccgagcgaa gcgacccgtg aagacgcgcg atgcgcgccc
     2701 ccgtcagatg gagttccgtc ttctgcgcgg gggtctgcgt cattgccgcc tcgaggaagt
     2761 gctctggcgt cagcgcgagg aagtagaaac cgtacgtcgg gtagcggatc tggttccaga
     2821 gaagggcggc cacgacgaac gcggccgcgc ccgccgtcgc gaggcgccct cttcgggcga
     2881 acgcacgcat ccactcgagg ccttccgcga ggaagcacac gaggaacgga aggagataga
     2941 cgagaaaacg cctgtcagcc cagtcgtaca cgaagagcca gaacgccgaa agagcgagaa
     3001 gcggcgcgaa caccgccgcc cggtaacgcc gcgccgtctc gtcgcgcgcg tcgaggcgga
     3061 aggcgcccgc cacgtagagg ggaaggacga ggaggccgag caccgcgggg agcgcgaccg
     3121 catagaggga gaggttcgag agattcgggc ggatcagggt ctcgacgccg tggcgcagga
     3181 gccccgggtg cgcgcggtaa tacgaggccc ggaacgcgac ccacgctccc agcggcgcgc
     3241 ctgccgccag gacgccccac cagaggctgc ggcgccggac gtccgcgggc ctcaggatga
     3301 ggaccgccac ggcgaggccg agcgccgaga gcgccgccgc gtaggagaag agaacgcctg
     3361 cgccgagggc ggcgcccatc gccacgtagg cgcgcggatc gcggccggcc cgaacgaagg
     3421 cgagtgccgc gaggacgagg aacggggtgc cgtaggtctc cgccatcacg tagcgcgcga
     3481 ggtcctgaac cgcgtcgttc gcgaagaaga accagcacgc cagcgccgcg atccatccgt
     3541 cgtggcgctc acgcaggagg agaaacagcg ccgccgtcga gagaccgaga aagaggaaat
     3601 tgacgacggg aaggaaagaa agtgcgccgg ccttccagag cgcggcgatc agaaggggca
     3661 ggcccggcgg cctcatcgtc gacgtgacgg gctcgcctgc caggaagagc ccgttcgaga
     3721 tccagtcata ggaatccgac gtcatgtacc ggtagcacca gttcaggtcc gggacgagga
     3781 gcgcgcgcac gaccggcaga gcgccggcca ggcagagcac ggcgagcgcc cctgcgggcc
     3841 ggcggtcgag gagctccgac actccgccga gtctacgggt acgtgaagta gaagggatcg
     3901 tagaggagcg tcacgtttcc cggagcgctg tggtactcct tcgtcagtcc gttgatcgtg
     3961 tccgtgaccg tgacccagaa ctccacggac gtgaacgtgg ggatcgcgat ccacgccttc
     4021 ccgttgtccg tcgccgtgtt gatccggatc atgtactcgt acgtcgcgcc gtcgaagagc
     4081 cagaaagcgc cggtcgcatc ggtgagcttc gccttgtaga ggtttgcggc cgcgccaccc
     4141 gcgtactggt tcttccatcg gctcgtgacg cgataccggc ccccgatgag acacatcgtg
     4201 ttcgcgtcct cgacgcagga gttgatcgtt gtgcacccgg tgccattgca cgacaccttc
     4261 ttgtccatga cgatccagtc cgcgtagtac caggtgccgc ttacgttctc actcaggaac
     4321 agcagctgcc agtactcgcc tgggggcatc gaactcccga aaaacgagac gctccccgag
     4381 ttgacctgtg aatattgata cccggcctga aggggcgaca acgaaagcgc gcctgagaat
     4441 gtataagcgc cgctgatcgt ctggccataa gcgggcaacg gattcgcgag aacgaccgtc
     4501 acgctcaacg gcccgctgaa gcccgagccc ctgttgttga cgacgcggtc cacgtggaac
     4561 gatccggaat tgccgttcca ggcaacagac gccgtgccgg ccaagcagat cgtgcagccc
     4621 ggctgggcga ggctctgcct cccgctctgg ttcgagccgg tcgacgggtc ttcacgtacc
     4681 ggctcggagc cgagagaaga cggggcagga ccgccggggt cgcctcccac gagcggataa
     4741 acgaaagtgc ccgggtcgta gatcagggtc tggttgccgg ccgggctgtg gtactgctgc
     4801 gactggcccg tcctcgtgtc ggtcacgtcg atccagaact ccacctgcgt gaacgtaggg
     4861 atcgcgatcc acgccttccc gttgtccgtc gccgtgttga tccggatcat gtactcgtag
     4921 gttgcgccgt cgaagagcca gaaggcgccg gtcgaatccg tgagcttcgc cttggaaagc
     4981 gtcgccacgg cgccgccggc gtactggttc ttccagtgag atcgaatccg gtagcggcca
     5041 ccgacgaggc acatcgtcgt cgcgtcctcg acacacgtgc tcgctcccga cggggcgacg
     5101 acgacggact tggtgatcgc gttcgagccg ccggagttcg aaaccgtcag cttgaccggg
     5161 aacgtgccgg ccgtcgcgaa ggcgtgggtc gcgttctgga ccgtcgaggc acccgcatcc
     5221 ccgaagtccc acgaccagga cgtggggctc ccggtcgacg cgtccgtgaa ttgaatcgtc
     5281 tggccggcca cgggagacgc agggttgaag ctgaagttgg ccacgggagc cgcgctgacc
     5341 gggttcgtca cgcgccagac gccgcggccg tatgtggctg cccagaggtc gttcgtgacc
     5401 ggatggaagg cgagctggta gacaggaacg ttcggcaggc cggcgttgaa cgtcgcccac
     5461 gaagcgccgc cgttcgtcga gcggaacacg ccgacgtcgc tgccgaggaa gatgtcggtg
     5521 gggtcggtcg ggtcgacctt gatgtcgaga atcgggacgt caggcaggtt gttcgagatg
     5581 ttcgtccacg tcacacccgc gtcagtcgtg cggaagacgt gcttcgaggg cagaccgaga
     5641 tagccggcaa acgtcgcgta cgcactccgg ccatcgggtg tcaccgaaac gacacgtgtg
     5701 acgggagccg ccggaagacc cgtgaagcgt gatgcgaatg tcgatccacc gtccgtcgag
     5761 aagaagatgc tgccagttgg tgtcccgccc cagacgggcc ccgggtctcc ccccggagcg
     5821 tccagcgtca tgaagtagga gttagtgatc ttgctcgatg atctcgcacc ccaccgcgcc
     5881 gcgtcctgac caaacgtgtc gttcgcccag attctgtttg tgcccagcaa caccgtccgc
     5941 ggctgcaacg agacggccgt cgccggtaca taaaaggaca tggactcaag cttctcgccg
     6001 gtggagcaat ccatgagctt tccgcagggt gtcgcgtcga cgaagtcggc cgccgtattg
     6061 ccggaatatt ccagaaacgc gtaatagtgc gctgaaagca cctggctcgg cgcgtcgcgg
     6121 cgaaagagac tgaagccgcc gtcgccttga gtgcgatcgc tccatgtcgc ggatcccgta
     6181 tacctttgat tgccattgtc ctgtgtccct ccgacggcga gattcacatc ggtgggatgg
     6241 agtgcgactc cgttgaactg tgcgagattc agcgaaaagt tcaaactctc gaaagacctc
     6301 gcgccatcga agctccgata gattcctccg tcgttaccga cgaacatggt cccgtcgggc
     6361 ccgaagacag ccgtatgctg gtcaggatgt gtgctcgcgg tcggtcccga gcctgaatag
     6421 acgttcaaga tattcgacca tgttgcgccc gcgtcagtgc tcttgtagag atcaatcgat
     6481 ccgaagtaca ccgttgaggg attggagggg tccggcgtga tccagtgatc gtagtgacat
     6541 tggttgttcc cgccagtagc cttagacgga cacattttcg gatcgaagga aacggcctgc
     6601 cacgtggctc caccgtcaag tgaccggtag atgcccccgc cgtccgatga atgggtgaag
     6661 tccgccgcga gcacggaggc gtagatcacc aacgggtcag tcttactcac gcccagcttg
     6721 atgttcgcga gcggagtggt cgtagtgaga cccggctgcc aagtcgttcc gaagtcggag
     6781 gaaacgaacc ctccatagga ggcacagccg ttacaccttc cagtcgtcat gaatatgcga
     6841 agtgggtttc cgggatcctg tactaggtcg ctgacacttc cgccagtgaa cgtctgagtg
     6901 aagtgaactc cgccgtcgac cgatctccag gttccaccca cgtagaatgt gttctgcgcg
     6961 acactgtggt aatagtaaat gccaacaaga actatggccg agttgttcgg ctgaacgacg
     7021 actcgcgaga ttattgcgtt agacggcaag ttgacgtcga tccggaccca cgtatccccg
     7081 gtatctgtcg acttaagtag ccccccacca aggtaggttc caagggagtc gttcggatag
     7141 ccttctgcaa gcgccttgtc ggcctctccg gtggccgcaa acgcaatcga cgggtccgaa
     7201 ggcgagaatg cgatgtggct gatggccaac gcagacgcgg tatctgacac tggccgccat
     7261 gtcgaaccgc cgtcggtaga cttccagatc ccgccggtcg ccccgcccag aagcatgacg
     7321 gcggggttcg tcggatgtat ggcgatggcg tctactcgcc cagcgacgac gccccaattc
     7381 cgacggccag aaggaaagga cgccatcggt tggggcccaa gtgactgcca agtcgtcccg
     7441 ctgaatgtgt agctcgagcg gacggaaggg cctaccgccg accgcgtaaa gctgcccacc
     7501 ggcccctgcg ccatcgaggg atcgacgggc atctggcagg tcgcatccag tgccttcagc
     7561 cggttctcgg aaagcacgcg cccgtcgcgg tcgcggcgca gcatcgcgtt ccattccgcg
     7621 cgttccgcag ggtggtcgcg gtcggcctcg cgcggttcct gcgcccgggt ggaaattgcg
     7681 gtggccagag ttaacgcggt cagcccaacg cgacaaaaca gggctagcag cttcggccgt
     7741 cccatcagga aaccctcctc ggattaatcg cgagaagtct attccaacca ctcggccacc
     7801 cgcgatatga ccttggatag gtcagccggg cggacatttg ctgtccttgc gtgcaaagaa
     7861 aaagggcccg gtttcccggg ccctcttttc gtttcgccga ttgcgcggcg ggcttacttc
     7921 tcgagctcga tgacgacgtc caccggcgag ccggcgcgtg ccgtgacctc gacggtcttg
     7981 gtcttgtacc cggggcgcgt gacggtgatc gtgtgcgtgc cgggctcggc gacgataccg
     8041 cgcgcgttcg ccgcgaggtc gtcggccgcg ccgaggtact tgtcgtccat gtagacggcc
     8101 gcgtcgtccg gcatcacacg gaacttgatc cgcccgcggt tcgtcgggcg cggcggcggc
     8161 gcgacgcgca cgtccgtgcg ctcttcgtcc ggggcctcgc gccaggacgg cgccgcggtg
//
LOCUS       DAOMFY010000167         8193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2719_length_8193_cov_8.325387, whole genome shotgun sequence.
ACCESSION   DAOMFY010000167 DAOMFY010000000
VERSION     DAOMFY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2719_length_8193_cov_8.325387"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLB01_12990"
     CDS             <1..516
                     /locus_tag="PLB01_12990"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQQ78262.1"
                     /translation="EIEAVFGAPVAEPGHPEAAVRAAIEMRARLESWNARRVAAGLAD
                     LLILALVGAVELAAGALLLDLRFPPPAFVPLAAFLFLAALVLLVLAPFVWGTTPGMAL
                     ADLRIRARDGGSPTLAAAFRRFLGFLLTGALAGVPLLVAAFDRRGRTLADLLSGTTIE
                     GVEALEPAGQS"
     gene            525..2219
                     /locus_tag="PLB01_12995"
     CDS             525..2219
                     /locus_tag="PLB01_12995"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HQQ78263.1"
                     /translation="MRLALGQIDSTIGDFAGNAAKIEAVLARAEEAGADLAAVPELAL
                     CGYPPRDLLERPSFQREAARTLAALAKRTKGTALLVGTFAPNRSRTGKPFHNVAAFLH
                     RGRVRVLAKKTLLPTYDVFDEGRWFEPGAGCAVVPFRGERLGLPVCEDLWNDKDFWRA
                     RRLYHEDPGDALVRRGATLIVSISASPFSEGKARLRRRMLARYARDGGVSVAYLNLVG
                     GNDELVFDGGSMVLDDRGRVGARAAHFEEDLLVADVVRGTGGRARVVAVSGGAPADAG
                     DDVEGEPLESLRRALVLGIRDYAAKCGFRTAVLGLSGGIDSALVAALAVDALGKENVT
                     GLGMPSPYSSEGSVADARTLAGNLGIRFEVLPIGGLFEDAKAALRPVFEGRPEDVAEE
                     NVQSRLRGLLVMGFSNKRGALVLTTGNKSELAVGYCTLYGDMCGGLAPISDLPKMRVY
                     ALARHLNARAGRDLIPLASLEKPPSAELRPGQTDQDSLPPYPVLDAILEGLVERNLTL
                     DAAARATGAPRALVERIARMMDLSEYKRRQAAPGLKVSAKAFGTGRRVPIAQRSPL"
     gene            2216..2914
                     /locus_tag="PLB01_13000"
     CDS             2216..2914
                     /locus_tag="PLB01_13000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ78264.1"
                     /translation="MSAASAGTPAYPWKEIPASSHVILLERVLARGSGLAVLDLGFGA
                     GHFAERIRPACRYLAGLELDPGAAAEGARFFDDAVTGDIFEGISRPWKEPFDVIVAGD
                     VLEHLARPEDLLATLKRLLKPDGTLLVSLPNVANVTVRAALLAGRFPYADRGILDRTH
                     LRFYTRAGARALLEQAGFRIRWETATAMPVELALPALGKPPLAAVVRGAARVSAAAWP
                     TLFGYQFVFEAAPA"
     gene            2911..3696
                     /locus_tag="PLB01_13005"
     CDS             2911..3696
                     /locus_tag="PLB01_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ78265.1"
                     /translation="MSAGALDTRTRQEAAPSRDTIDLSIVIPAYNEEARLPASLGTIA
                     AYLSARASGPRVEVLVVDDGSSDATGPRTQEAARRHGLSLRVLRLAQNRGKGAAVRAG
                     CLEAAGALVLVSDADFSTPVYEWEKLAAAGAPVAIGSRALDEGLVKEKQPLFRQAMGK
                     LFNRLVRLVAVPGIRDTQCGFKLFTREAAREIFSRAKVDRFAYDVEALLLARQLGYAI
                     AEVPVLWFNSADSRVTLFGGAQAYWDVLRIRFTTRAGRISRGA"
     gene            complement(3680..5095)
                     /locus_tag="PLB01_13010"
     CDS             complement(3680..5095)
                     /locus_tag="PLB01_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78266.1"
                     /translation="MRRRRILGILGGTVLAALVGAPRVVHAQPNITVSGSCDGAGNSS
                     FVIKNTGTAMTVNYTWEILQNGVFLTSGPFLLTAAGTTGDTLQLTINGLYGNITVNVR
                     DQSNVLVDSASAVCVTPTPTPTRTFTPTPIPPNVTVAGSCDGAGNSIFTIRNTGGAMT
                     VNYTWEIFQNGVFLTSGTFRLTAAGTTGDTLQLTINGLYGNITVGVKDGTAVTVASAT
                     AVCVTPTPTPTRTSTQTPTFTITPTFTITPTATVTPPRTPTSTATATSTSTPTPTPTP
                     TPTPTPTSTATPTPTPTPTATSTPTATPSATPTPIATSTFTSTLTPTPTRTPLPPGGA
                     VVVDRPKPAPGGTIYASGQVPVAGRAACLALVPNGTWAVGATFGGTAVVSALLPGGPS
                     ASFAHAPVGAAPASGAYDVLLLDGDCGASSPVILAAFAAGPDPGLDMSLPDVPAVGSA
                     GLLALAALLALAALPLLRRLG"
     gene            complement(5114..6394)
                     /gene="glyA"
                     /locus_tag="PLB01_13015"
     CDS             complement(5114..6394)
                     /gene="glyA"
                     /locus_tag="PLB01_13015"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQQ78267.1"
                     /translation="MSTAVRPAALLNRSLAEADPEIARIVAAETARQNEGIELIASEN
                     FTSRAVMEAVGSTLTNKYAEGYPGKRYYGGCVHVDEAESLAIARAKTLFSPGNPDAVH
                     ANVQPHAGSQANTAVYVACLKPGDTMMGMSLAHGGHLTHGHPLSISGKLYRIVSYGVG
                     KDTETIDWDAFEATAKAEKPKLIIAGASAYPRTLDFPRFRAIADAVGALLMVDMAHIA
                     GLVAAGVHPSPVGHAHFVTTTTHKTLRGPRSGLILCVPEWAKEIDKAVFPGLQGGPLE
                     HVVAGKAVAFHEAATPEFAAYQKQVVANAKVLAASVAAEGFRLVSGGTDNHLFLVDVG
                     KKGLTGKVAEKALDAAGITVNKNAIPFDPNPPMVTSGLRIGTPAVTTRGMGEGEMKTI
                     GGLIAEVLAAPEDAAVQARVKAKVRDLTAAFPLY"
     gene            complement(6416..7777)
                     /gene="dgt"
                     /locus_tag="PLB01_13020"
     CDS             complement(6416..7777)
                     /gene="dgt"
                     /locus_tag="PLB01_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HQQ78268.1"
                     /translation="MSEPSGKNLVPAYDKKALEALEDARLAVYASRASRASRRYPVAE
                     EGKTYDYRTEYQRDRDRIIHSRAFRRLKHKTQVFIPFEGDHYRTRLTHTLEVTQVART
                     VARALGLNEDLAEACALGHDLGHTPFGHSGEKVLNRLLAGGDPALPLDGKLAAQVGAF
                     KHNYQAVRVVDVLEKRYEHPGLNLTNDTREGILKHTTWRRDFPFPDVDAEGLNLSSGG
                     HLEGQAVAWADEYAQQAHDLEDGLKLVDFEKVLGLSVAKAVVEALGPAWTGATDRTVR
                     EGMLIRGMIHLLVTDLITSSRAAIGKWLEAKSVKTPDDFYAQRRKLPGSLVAPSKTGQ
                     RFYDELKEFIYQHIIHSHRVAQHDGRAQLVVAALFSAWYRNPRLLPDYVLLRYRDLAG
                     VTYLRDVPLKRLEAEIAANYLRKPLFLRSIADHLAGMTDSYALSEYDSMFSAYPRHAP
                     GTP"
     gene            complement(7787..>8193)
                     /locus_tag="PLB01_13025"
     CDS             complement(7787..>8193)
                     /locus_tag="PLB01_13025"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQQ78269.1"
                     /translation="RWRARLERIESPGRRPLLVDGAHNPPGAAALAAYLDMAELSGQV
                     DLLFGGMADKDLAAVFRPLAARARRIVLVAPASPRAEGAESLAGRLGRPDLEKAASVA
                     EGLALLEAAGGSAPILVAGSLYLAGEVLTLRG"
BASE COUNT         1097 a         2873 c         2966 g         1257 t
ORIGIN      
        1 gagatcgagg cggtgttcgg tgcgccggtg gccgagcccg gccacccgga agccgcggtg
       61 cgcgcagcca tcgagatgcg cgcacggctc gaatcgtgga acgcgcgccg cgtcgcggca
      121 gggctcgcgg acctgctgat cctcgcgctc gtcggagcgg tcgagcttgc cgcgggcgcg
      181 ctcctcctcg acctgcgctt cccgccgccg gcgttcgtgc cgctcgcggc cttcctcttc
      241 ctcgcggcgc tcgtcctcct cgtcctcgcg cccttcgtct ggggcacgac gccggggatg
      301 gcgctcgcgg accttcgcat ccgggcgcgc gacggcggat cgccgacgct cgccgcggcg
      361 ttccggaggt tcctcggctt cctgctgacg ggagcgcttg ccggcgtccc gctcctcgtc
      421 gccgccttcg accgccgcgg gcgcactctc gcggacctgc tcagcggcac gacgatcgaa
      481 ggcgtcgagg cgctcgaacc ggccggacag tcgtaggggg ccgcgtgcgg ctcgcccttg
      541 gccagatcga ctcgaccatc ggggatttcg cgggcaacgc cgcgaagatc gaggccgtcc
      601 tcgcccgggc cgaagaggcc ggcgccgacc tcgccgcggt gccggaactc gcgctctgcg
      661 gctacccgcc gcgcgacctc ctcgagcgcc cgtcgttcca gcgcgaagcg gcgcgaaccc
      721 tggccgcgct cgcgaagagg acgaagggca ccgcgctcct cgtcgggacc tttgccccga
      781 atcgcagccg gacgggcaag ccgttccaca acgtcgcggc tttcctccac cgcgggcgcg
      841 tgcgcgtcct cgcgaagaag acccttctcc cgacgtacga cgtcttcgac gagggacgct
      901 ggttcgaacc gggcgccggc tgcgccgtcg tgcccttccg aggggagcgg ctcgggctgc
      961 ccgtttgcga ggacctctgg aacgacaagg acttctggcg cgcgcggcgc ctctaccacg
     1021 aggacccggg cgacgcgctc gttcggcggg gagcgacgct catcgtgtcg atctccgcgt
     1081 cgccgttctc cgaagggaaa gcgcggctgc ggcgacggat gctcgcgcgc tacgcgcggg
     1141 acggcggcgt gagcgtggcg tacctgaacc tcgtcggcgg caacgacgag ctcgtcttcg
     1201 acggaggctc gatggtgctc gacgaccgcg gccgtgtcgg cgcgcgcgcc gcgcacttcg
     1261 aggaagatct cctcgtcgcg gacgtcgtgc gcgggaccgg cggacgcgcg cgcgtcgtcg
     1321 ccgtgtcggg cggcgcgccg gcggacgccg gcgacgacgt ggagggcgag ccgctcgagt
     1381 ccctgcgccg cgcgctcgtc ctcgggatcc gcgactacgc cgccaagtgc ggattccgga
     1441 cggccgttct cggcctttcg ggcggaatcg actcggcgct cgtcgcggcg ctcgccgtgg
     1501 acgcgctcgg gaaggaaaac gtcacgggcc tcgggatgcc ttccccgtac tcgtcggagg
     1561 gctcggtcgc ggacgcgcgc acgctcgccg gaaatctcgg gatccgcttc gaggtgctgc
     1621 cgatcggagg gctcttcgag gacgcgaagg ccgcgctccg gcccgtcttc gaggggcgcc
     1681 ccgaggacgt cgcggaggag aacgtccagt cgcgcctccg cgggctgctc gtgatgggct
     1741 tctcgaacaa gcggggcgcg ctcgtcctga cgaccgggaa caagagcgag ctcgccgtcg
     1801 gctactgcac cctctacggc gacatgtgcg gcggcctcgc gccgatctcg gacctcccga
     1861 agatgcgcgt ctacgcgctc gcgcggcacc tcaacgcgcg cgccggccgc gacctcattc
     1921 cgctcgcctc gctcgagaag ccgccgtccg cagagctccg gcccggccag acggaccagg
     1981 actctcttcc gccgtacccc gtcctcgacg cgatcctcga gggcctcgtg gagcgcaacc
     2041 tcacgctcga cgcggccgcg cgcgccaccg gcgccccgcg cgcgctcgtg gagcggatcg
     2101 cgcggatgat ggacctctcc gagtacaagc gccggcaggc cgcgccgggc ctgaaagtct
     2161 cggcgaaggc gttcggcacc gggcgccgcg tgccgatcgc ccagaggtcg cccctgtgag
     2221 cgccgcgtcc gcgggaacgc cggcgtatcc ctggaaggag attccggcgt cgtcgcacgt
     2281 catcctcctc gaaagagtgc tcgcgcgggg aagcggcctc gccgttctcg acctcgggtt
     2341 cggggccggc cacttcgcgg aacgcattcg cccggcgtgt cgatacctcg cggggctaga
     2401 gctcgatccg ggcgcggctg cggaaggcgc ccgcttcttc gacgacgccg tgaccggcga
     2461 catcttcgaa ggaatctcca gaccctggaa agagccgttc gacgtcatcg ttgcgggcga
     2521 cgtcctcgag catctggccc gcccggaaga tctcctcgcg acgctcaagc gactcctgaa
     2581 acccgacgga acgctcctcg tctcgctccc gaacgtcgcg aacgtgacgg tgcgtgcggc
     2641 gctgctggcc ggacggtttc cctacgcgga ccgcggcatc ctcgaccgca cgcacctgcg
     2701 cttctacacg cgcgccggcg ctcgcgccct gctcgagcag gcggggttcc ggattcgatg
     2761 ggagaccgcg accgcgatgc ccgtcgagct cgcgctcccg gcgctcggaa agccgcctct
     2821 cgccgcggtg gtccgcggcg cagcccgggt ttccgccgcc gcctggccca cgctcttcgg
     2881 ctaccagttc gtcttcgagg ccgctcccgc atgagcgccg gcgccctcga cacccggacg
     2941 cggcaggagg ccgcgccttc ccgcgacacc atcgacctct cgatcgtgat cccggcgtac
     3001 aacgaggaag cccggctgcc cgcgtccctc gggacgatcg cggcgtacct ctcggcccgg
     3061 gcgtccggcc cgcgcgtcga ggtcctcgtc gtggacgacg gctcctcgga cgccacgggg
     3121 ccgcggaccc aggaggccgc gcgccggcac gggctgtccc tgcgcgtcct caggctcgcc
     3181 cagaaccgcg gcaagggcgc cgcggtgcgg gcgggctgcc tcgaggcggc cggcgcgctc
     3241 gtcctcgttt cggacgccga cttctcgacg ccggtttacg agtgggagaa gctcgccgcg
     3301 gccggcgcgc ccgtcgcgat cggatcgcgc gcgctcgacg agggactcgt gaaggagaag
     3361 cagcccctct tccggcaggc catgggcaag ctgttcaacc ggctcgtacg cctcgtcgcc
     3421 gtgcccggca tccgcgacac gcagtgcggc ttcaagctgt tcacgcgaga ggcggcgcgc
     3481 gagatcttct cgcgcgcgaa ggtggaccgc ttcgcgtacg acgtcgaggc gctgctcctc
     3541 gcgcggcagc tcggctacgc gatcgccgag gtgcccgtcc tctggttcaa ctcggcggat
     3601 tcgcgcgtca cgctcttcgg cggcgcgcag gcctactggg acgtgctcag gatccgcttc
     3661 acgacccgcg ccggacggat cagccgaggc gcctgaggag cggcagcgcc gcgagggcga
     3721 ggagcgccgc aagcgcgagg aggcccgcgc ttccgaccgc cgggacgtcc ggcagagaca
     3781 tgtcgaggcc ggggtcgggg ccggcggcaa acgcggcgag gatcacgggc gacgacgccc
     3841 cgcagtcgcc gtcgagaagc aggacgtcgt acgcacccga ggcgggcgcc gccccgacgg
     3901 gcgcatgcgc gaaggacgcc gaggggccgc cgggaagcaa agccgagacg acggccgttc
     3961 ctccgaaggt cgctccaacc gcccacgtcc cgttcggcac gagtgcgagg catgcggccc
     4021 ggcccgcgac gggcacctgg cccgacgcgt agatcgtccc gcccggtgcc ggcttcggcc
     4081 ggtcgacgac gacggccccg ccgggaggca gcggcgttct cgtcggtgtc ggcgtgagcg
     4141 tggacgtgaa cgtggacgtc gcgatcgggg tcggcgtggc actcggcgtt gccgtcgggg
     4201 tagacgtggc ggtcggcgtg ggagtgggcg tcggcgttgc cgtcgaggtg ggcgtcgggg
     4261 tcggcgtggg cgtgggagtc ggcgtgggcg tcgacgtgga cgtcgcggtc gctgtgctcg
     4321 tcggtgtgcg cgggggtgtg accgtcgccg tgggcgtgat cgtgaacgtc ggcgtgatcg
     4381 tgaacgtcgg cgtttgcgtc gacgtgcgcg tcggcgtcgg tgtcggcgtc acgcagacag
     4441 cggtcgcgga cgcgacggtc acggccgtgc cgtccttgac cccgacggtg atgttcccat
     4501 agagcccatt gatcgtgagt tgcagcgtgt cccccgtggt gccggccgcc gtcagcctga
     4561 acgtgcccga ggtaaggaac acgccgttct ggaagatctc ccatgtgtag ttcacggtca
     4621 tcgccccgcc ggtgttcctg atcgtgaaga tcgagttccc ggcgccatca caggacccgg
     4681 ccaccgtcac gttcggcgga atcggcgtgg gcgtgaatgt ccgagtcggc gtcggtgtcg
     4741 gggtgacgca gaccgcggag gccgagtcga cgagcacgtt ggactggtcg cggacattca
     4801 cggtgatgtt gccgtagagg ccgttgatcg tcagttggag agtgtcaccc gtggtgcctg
     4861 ccgccgtgag caggaacggg cccgacgtga ggaacacgcc gttctggaga atctcccacg
     4921 tgtagttcac ggtcatcgcc gtgccggtgt tcttgatcac gaagctcgag ttcccggccc
     4981 cgtcgcacga acccgagacc gtgatgttcg gctgcgcatg gacgacgcgc ggcgctccga
     5041 cgagcgccgc gagcacggtt ccaccgagga tcccgagaat ccgccgtctt ctcacgaacc
     5101 cggggactct aactcagtag agaggaaacg ccgccgtcag gtcgcggacc ttcgccttca
     5161 cgcgcgcctg tacggccgcg tcctcgggcg cggcgaggac ctcggcgatg aggccgccga
     5221 tcgtcttcat ctcgccctcg cccatcccgc gcgtcgtgac ggccggcgtg ccgatgcgca
     5281 ggcccgacgt caccatcggc gggttcgggt cgaacggaat cgcgttcttg ttgacggtga
     5341 tcccggccgc gtcgagcgcc ttctcggcga ctttgcccgt gaggcccttc ttgccgacgt
     5401 ccacgaggaa gaggtggttg tcggtcccgc ccgacacgag gcggaacccc tcggcggcga
     5461 cgctcgccgc gaggaccttc gcgttcgcga cgacctgctt ctggtaggcc gcgaattccg
     5521 gcgtcgcggc ctcgtggaac gcgactgcct tgcccgcgac gacgtgctcg agcggcccgc
     5581 cctgcagtcc cggaaagacg gccttgtcga tctccttcgc ccactccggg acgcagagga
     5641 tcaggcccga gcgcggcccg cgcagcgtct tgtgcgtcgt cgtcgtgacg aagtgcgcgt
     5701 gcccgaccgg cgacggatgg acgcccgccg ccacgaggcc cgcgatgtgc gccatgtcga
     5761 ccatgaggag cgcgccgacg gcgtccgcaa tggcgcggaa gcgcgggaag tccagcgtgc
     5821 gcgggtacgc ggacgctccc gcgatgatga gcttcggctt ctcggccttc gccgtcgcct
     5881 cgaaggcgtc ccagtcgatc gtctcggtgt ccttgccgac gccgtacgag acgatgcggt
     5941 agagcttccc cgagatcgag agcgggtggc cgtgcgtgag gtggccgccg tgcgcgagcg
     6001 acatccccat catcgtgtcg ccgggcttga ggcacgcgac gtacacggcc gtgttcgcct
     6061 gcgagcccgc atgcggctgg acgttcgcgt ggaccgcatc cgggttcccg ggcgagaaca
     6121 gcgtcttggc gcgcgcgatc gcgagggact cggcctcgtc cacgtgcacg cagccgccgt
     6181 agtagcgctt ccccgggtag ccctcggcgt acttgttcgt gagcgtagag ccgacggctt
     6241 ccatcacggc gcgcgacgtg aagttctccg aggcgatgag ctcgatcccc tcgttctggc
     6301 gcgccgtctc cgcggcgacg atgcgggcga tctcgggatc ggcctcggcg agggagcggt
     6361 tcaggagggc ggcgggacgg acggcggtcg acatgggtcc tccggagccg cgatcttacg
     6421 gcgtcccggg cgcgtggcgc gggtacgccg agaacatcga gtcgtactcc gagagcgcgt
     6481 agctgtccgt catcccggcg agatggtccg cgatggagcg caggaacagc ggcttcctga
     6541 ggtagttcgc cgcgatctcg gcttcgagcc gcttgagcgg cacgtccctc aggtacgtca
     6601 cgcccgcgag gtcgcggtag cgcaggagaa cgtagtccgg caggaggcgc ggattccggt
     6661 accacgccga aaagagcgcc gccacgacga gctgcgcgcg cccgtcgtgc tgcgcgacgc
     6721 gatggctgtg gatgatgtgc tggtagatga actccttgag ttcgtcgtag aagcgctggc
     6781 ccgtcttcga cggagcgacg agcgagcccg ggagcttcct ccgctgcgcg tagaaatcgt
     6841 cgggcgtctt gacggacttc gcctcgagcc acttcccgat ggccgcgcgc gaggacgtga
     6901 tcaggtccgt cacgaggagg tggatcatcc cgcggatcag cattccctcg cgcaccgtcc
     6961 ggtccgtcgc ccccgtccat gccggtccga gagcctcgac gacggctttc gcgacggaca
     7021 aaccgagcac tttctcgaaa tccacgagct tcagcccgtc ttcgaggtcg tgcgcctgct
     7081 gggcgtactc gtcggcccac gcgacggcct ggccttcgag atggccgccc gaggagaggt
     7141 tgagcccctc ggcgtccacg tcgggaaacg gaaaatccct ccgccacgtc gtgtgcttga
     7201 ggatgccttc acgcgtgtcg ttcgtgaggt tcaggcccgg atgctcgtag cgtttctcga
     7261 ggacgtcgac gacgcgcacg gcctggtagt tgtgcttgaa ggccccgacc tgcgccgcga
     7321 gcttgccgtc gagcgggagt gcgggatcgc cgccggcgag gaggcggttc agaactttct
     7381 cgcccgagtg gccgaacggc gtgtgcccga gatcgtgccc gagcgcgcaa gcctccgcca
     7441 ggtcctcgtt caggccgagg gcacgtgcga ccgtccgcgc gacctgggtg acctcgagcg
     7501 tgtgcgtgag ccgcgtgcgg taatggtcgc cctcgaacgg gatgaagacc tgggtcttgt
     7561 gcttgagccg gcggaacgcg cgcgagtgga tgatccggtc gcggtcccgc tggtactcgg
     7621 tccggtagtc gtaggtcttc ccctcttcgg caacggggta acggcgcgag gcgcgcgagg
     7681 cgcgcgaggc gtagacggcg aggcgggcgt cttccagggc ctcgagggcc ttcttgtcgt
     7741 aagcggggac gagattcttc ccggagggtt cgctcacgcg gggatcttag cctcgaagag
     7801 tgagcacctc gccggcaaga tagagcgagc ccgcgacgag gatcggggcg gagccacccg
     7861 ccgcctcgag gagcgcgagc ccttcggcga cggatgccgc cttctcgagg tcgggccgcc
     7921 cgaggcgtcc ggcgaggctc tccgcgccct ccgcgcgcgg cgaggcggga gccacgagga
     7981 cgatccggcg cgcgcgcgcg gccagcggcc ggaagaccgc ggcgaggtcc ttgtcggcca
     8041 tcccgccgaa gaggaggtcg acctgtcccg agagttccgc catgtcgagg tatgcggcca
     8101 gtgcggccgc gcccggcggg ttgtgcgcgc cgtccacgag gagcggtcgc cgcccggggc
     8161 tctcgatccg ctcgagccgc gcgcgccagc gcg
//
LOCUS       DAOMFY010000168         8191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2723_length_8191_cov_7.545354, whole genome shotgun sequence.
ACCESSION   DAOMFY010000168 DAOMFY010000000
VERSION     DAOMFY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2723_length_8191_cov_7.545354"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PLB01_13030"
     CDS             complement(<1..1195)
                     /locus_tag="PLB01_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78270.1"
                     /translation="MSAAPDAGASRRRLVTGLLLAGVAIQIAGLLVSAAGTDLRTPQS
                     EDMVPFTYLLATIASVLAAVVAMAGSVLILSLTLGTAVQRSHLGSMILADEDFRTAVS
                     GAIVTIVAACLALLAAHLPPVSHNPTAIFLLSAGGVLEFGSCILGLARLFQRIPRLLT
                     QESLLENSLAHVDAQYLAAQEDRLLGAGATSESRREVLNDLIFSFRVYPVETDDPLVT
                     VHDVLENVLAQSPASVVATSLAQVSARFTALSGPTHDQIFQLLVTPWLAELRVAVVKR
                     GSPSVALAFFDLWADVLVAARERKMPGFYAHSARPFVASLRHVAHAGLFEEAGGRVLR
                     PLVRVMAAARADLPALGPWAGALVGWMREDAEKGRGEWTRVLWNPVEDVLERSLEDLG
                     HDTPLL"
     gene            complement(1192..2403)
                     /locus_tag="PLB01_13035"
     CDS             complement(1192..2403)
                     /locus_tag="PLB01_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78271.1"
                     /translation="MHPENRRAFNAAFTPALAAGYADDLVRRAGRAPGFRLAETPVFL
                     TPELDAALVKAAREIVDQLSAPGLLARAKSAIPARWDAPGMDALPSLTQVDFAVVRHE
                     GRLVPRLIELQGFPSLSAFEVLQADAWNAALESVPGLPKGAWSPYFSGLDRAGFVDLF
                     RRTIVGGEDPAEVVLLDLEPEKQKTAIDFAATRVLLGVDSVDPRALVKVGRGLFRDVD
                     GRRVPVKRIYNRIVFDELIQTGAVLPFDFREELDVRWVPHPNWYWAWSKACLPLLAHE
                     AAPKATVVSAFGPVPDDVGERYVLKPLFSFAGGGVNVDPTPADVARIPEAERAHWVLM
                     EKIAYDPCLRAVDGGGVKVEVRMMFFRPDGEAAPVLALNLCRLSRGKMLGVDFNKDFT
                     WVGSSVALKPA"
     gene            complement(2415..3827)
                     /locus_tag="PLB01_13040"
     CDS             complement(2415..3827)
                     /locus_tag="PLB01_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQQ78272.1"
                     /translation="MSPPEAPPPAPGVLATLREALRGGHGDFTEGPVGRAVVLLAIPM
                     VLEMALESVFAVVDVFWVSKLGAAAVATVGLTESILSILYSLAIGLSMAATAMVARRI
                     GERDRESAARTAVQAIALGVLVAVPLGVAGALLAPRLLAFMGAGPDVVASGSGFMAVM
                     LGGNVVIVLLFLVNAIFRGAGDATVAMRVLWFANVCNLVLDPCLIFGLGPFPALGVTG
                     AAVATTTGRGLAVVLQLVILARGTGRIAIRREHLALDPKTMLALLKISGAAIFQSLIG
                     TASWIGLVRILAGFGSAALAGYTIAIRIVIFAILPSWGMSNAAATLVGQNLGAKRPDR
                     AERSVFFAGVYNMAFLGAVGAVFAAFPGALVGLFTADPAILPIASNGLRVVAAGFPFF
                     AWGMVLVNAFNGAGDTWTPTVVNLGVFWLWEIPLAHVLASRAGMGPRGVFLAITIGFS
                     TLAVVGGLLFRRGKWKEKKV"
     gene            complement(3846..4949)
                     /gene="ychF"
                     /locus_tag="PLB01_13045"
     CDS             complement(3846..4949)
                     /gene="ychF"
                     /locus_tag="PLB01_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQQ78273.1"
                     /translation="MLRAGIVGLPNVGKSTLFNALTKTRKAEAANYPFCTIEPNVGIV
                     TVPDARLEPLARIAKTPVKIPATFEFVDIAGLVKGASKGEGLGNKFLSHIREVDAVVQ
                     VVRCFEDKDIVHVSETLDPVADIETVATELVLADLESLGKQKERLEKLARGGQKEAKA
                     QLAVAEKVLPHLDGGKPALTLDLTAEEKAAARAFFLLTMKPTLFACNVAEADLAHADD
                     LPLVKKVRDWAAAHSGSEAVAISAKIEADLAELSPEDAAEYLASHGVDESGVGRLIRA
                     AYHLLGLRTYFTAGEKEARAWTIHAGDTAPQAAGVIHSDFEKGFIKAETIAYDDLVTH
                     GSMAAAKEAGVVRQEGRDYVVKDGDVMLFKFNV"
     gene            5018..5503
                     /locus_tag="PLB01_13050"
     CDS             5018..5503
                     /locus_tag="PLB01_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78274.1"
                     /translation="MNETPRLRVFTLGLALLALAAAPADGAKKPSKKSPHTQIVAVCR
                     VETAPAEKTHKNGRTFLEFDVTIVSYERVKGDAAADPALAIRQDRRIHVVHDLSCGGE
                     RLALAKGDAAEIRGEYVAPDNGKDLIHFTHPADGSCGTKGGHPDGGIRKLDAAALKKA
                     A"
     gene            5500..7218
                     /gene="aceK"
                     /locus_tag="PLB01_13055"
     CDS             5500..7218
                     /gene="aceK"
                     /locus_tag="PLB01_13055"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HQQ78275.1"
                     /translation="MSPDDAGRAGAGRLLAAWDAYSREFAAITRRAPVRFSARDWRGV
                     RADALERLELYRKMLDPAVEDLGRILGPGVGNPAAWSGMKERFAALVAGRKDVELVET
                     FFNSASRRIFHTVGVDDRAEFVHREPTPLPVEAAGRFLASFDGRPGREAAVRGALSVS
                     PFAAGWEDLTRDAALATRALDRVLGAAWGSPAFDALDVLRPVFFRGKGAYLVGRVRRE
                     GRARPVVFALLNRTGRVEVDAVLCDEDEASIVFSFTRSYFHVDAEQPAALVGFLKTLM
                     PKKPVGELYNALGFNKHGKTELYRDLLRHLAASDDRFVEAPGARGMVMLVFTLPSYDV
                     VFKVIRDSFAPPKTASRKDVLDKYRLVFRHTRAGRLADVQEFEHLSFPVARFDAPLLE
                     TLLAGAAETVEVRGDEVVLRHLFTERRLTPLDIALKEASPAQARAAILDFGEAIRDLA
                     KANVFPGDLLPKNFGLTRHGRVVFYDYDEIDFLTDVNFRELPAGADEEAGGDDASFFV
                     GEHDVFPEEFLMFMGLRGPAREAFLERHADLLGVRFWLEAQRRERAGEIVDVYPYPLE
                     ARLRID"
     gene            complement(7205..>8191)
                     /locus_tag="PLB01_13060"
     CDS             complement(7205..>8191)
                     /locus_tag="PLB01_13060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ78276.1"
                     /translation="RRLPRLQEPPARRAPVTARATAAVVIPSFQHARWVGEAVESALS
                     QTRPPDEVVVVDDGSTDGTLDVLAGFGGRIRVLENARGGIGSTYNMGVAAVKADVIAF
                     LESDDVLEPRYLEETEDFLEGNREIPWVSTARLLIDASGAPTGEISRRRLPGAEFTFE
                     GFLSSELGASSTPVVRRAALLEAGPYSTDSYAADTDMALRFSLAHRMGYLDRPLYRYR
                     RHGENASASLLESVRLNVPILERLLDPPHPALAGREGAVKKALAKMLGMQAALAMESG
                     SGGAREEVLPLLKRAAALHPSPKHLRRLALVGLVGPRTYGAWRRARPLISRS"
BASE COUNT         1252 a         2900 c         2944 g         1095 t
ORIGIN      
        1 cgagaagcgg cgtgtcgtgc ccgaggtcct cgagggagcg ttcgaggacg tcctcgaccg
       61 ggttccagag cacgcgcgtc cactcgccgc ggcctttctc ggcgtcctcg cgcatccagc
      121 cgacgagcgc gcccgcccac gggccgagcg ccggcaggtc ggcgcgcgcc gcggccatca
      181 cgcggacgag gggccggagc acgcgtccgc ccgcctcctc gaagagcccg gcatgcgcca
      241 cgtgccgcag cgaggccacg aacggacgcg ccgagtgcgc atagaacccc ggcatcttcc
      301 gctcgcgcgc ggcgacgagg acgtccgccc acagatcgaa gaacgcgagc gccacggacg
      361 gcgagccgcg cttgacgacc gcgacgcgca gctcggcgag ccacggcgtc acgagcagct
      421 ggaagatctg gtcgtgcgtg ggcccggaga gcgccgtgaa acgcgccgag acctgggcga
      481 gcgacgtcgc gacgacggac gccggactct gggcgagcac gttctcgagg acgtcgtgca
      541 cggtcacgag cgggtcgtcc gtctcgaccg ggtagacccg gaacgagaag atcaggtcgt
      601 tcaggacctc gcgccggctc tccgacgtcg cgcccgctcc gaggagccgg tcttcctgcg
      661 ccgcgaggta ctgcgcgtcc acgtgggcga gagagttctc gaggaggctc tcctgcgtga
      721 gaagccgcgg gatgcgctgg aagagccgcg cgagaccgag gatgcagctg ccgaactcga
      781 ggacgccgcc agccgagagc aggaagatcg ccgtcgggtt gtgcgagacg ggcggcagat
      841 gcgcggcgag gagcgcgagg caggccgcga cgatcgtcac gatcgcgccc gacacggccg
      901 tccggaagtc ctcgtcggcg aggatcatcg agccgaggtg cgagcgctgg acggcggtcc
      961 cgagcgtgag cgagaggatg aggacggagc ccgccatcgc gacgaccgcg gcgagcacgg
     1021 acgcgatcgt cgcgaggaga tacgtgaacg ggaccatgtc ctcggactgc ggcgtgcgca
     1081 ggtcggttcc ggccgccgac acgaggaggc cggcgatctg gatcgcgacg cccgcgagaa
     1141 ggaggccggt gacgagccgg cggcggctcg cgcccgcgtc gggagcggcg ctcacgccgg
     1201 cttgagggcc acggacgacc cgacccacgt gaagtccttg ttgaagtcga cgccgagcat
     1261 cttcccgcgc gaaaggcggc agaggttcag cgcgagcacg ggcgcggctt cgccgtcggg
     1321 ccggaagaac atcatgcgga cctcgacctt cacgccgccg ccgtccacgg ccctgaggca
     1381 cgggtcgtac gcgatcttct ccatcaggac ccagtgcgcc cgctcggcct ccgggatccg
     1441 cgcgacatcc gcaggcgtcg ggtccacgtt cacgccgccg cccgcgaacg agaagagcgg
     1501 cttcaggacg tagcgttccc cgacgtcgtc cggcaccggc ccgaacgcag agacgacggt
     1561 cgccttcggg gcggcttcgt gcgcgaggag agggagacac gccttcgacc acgcccagta
     1621 ccagttcggg tgcgggaccc agcggacgtc gagctcctcc cggaagtcga acgggagcac
     1681 ggcgccggtc tggatgagct cgtcgaagac gattcggttg tagatgcgct tcacgggaac
     1741 gcgccgtccg tccacgtcgc ggaagaggcc gcgtccgacc ttcaccaggg cgcgcgggtc
     1801 gacgctgtcc acgccgagga gcacccgtgt cgccgcgaaa tcgatcgccg tcttctgctt
     1861 ctcgggctcg aggtcgagaa gaacgacctc ggcgggatcc tcgccgccga cgatcgtgcg
     1921 gcggaagagg tcgacgaacc cggcccggtc gaggcccgag aagtacgggc tccacgcgcc
     1981 cttcgggagg cccggcacgc tctcgagcgc cgcgttccag gcgtccgcct ggagcacctc
     2041 gaacgccgag agcgacggga agccctgcag ctcgatgagg cgggggacga gccgcccttc
     2101 gtgccgcacg acggcgaagt ccacctgcgt gagggaggga agcgcatcca tcccgggagc
     2161 gtcccagcgc gccgggatcg cgctcttcgc ccgggcgaga aggcccggcg ccgagagctg
     2221 atcgacgatc tcgcgggccg ccttcacgag cgccgcgtcg agctccggcg tcaggaacac
     2281 cggcgtctcc gcgaggcgga agcccggcgc gcggccggcg cgtcgcacga ggtcgtccgc
     2341 atagcccgcc gcgagcgcag gcgtgaaggc cgcgttgaac gcgcggcggt tttccggatg
     2401 catgggcgcg gagtttagac cttcttctcc ttccacttcc cgcgccggaa gagaagcccg
     2461 cccacgacgg caagcgtcga gaacccgatc gtgatcgcga ggaacacgcc gcgcgggccc
     2521 attcccgcgc gcgaggcgag gacgtgcgcg agcgggatct cccacagcca gaagacgccg
     2581 agattcacga ccgtcggcgt ccaggtgtcg ccggccccgt tgaacgcgtt cacgagcacc
     2641 attccccacg cgaagaacgg aaagcccgcg gcgacgacgc ggagcccgtt ggaggcgatc
     2701 gggaggatcg cggggtcggc cgtgaacagg cccacgagcg ctccgggaaa cgcggcgaag
     2761 accgccccta ccgcgccgag gaacgccatg ttgtagacgc ctgcgaagaa gacggagcgc
     2821 tcggcccggt cgggccgctt cgcgccgagg ttctggccga cgagcgtcgc ggccgcgttc
     2881 gacattcccc acgacggcag gatcgcgaag atcacgatcc ggatcgcgat cgtgtatccc
     2941 gcgagcgccg cgctcccgaa gcccgcgagg atccgcacga ggccgatcca gctcgcggtc
     3001 ccgatcaacg actggaagat cgcggcgccc gagatcttca ggagcgcgag catcgtcttc
     3061 gggtccagcg cgaggtgctc gcggcggatc gcgatgcggc cggttccgcg cgcgaggatc
     3121 acgagctgga ggacgaccgc gaggccgcgg cccgtcgtcg tggcgacggc agcgcccgtg
     3181 acgccgagag cgggaaacgg cccgaggccg aagatgaggc acgggtcgag gacgaggttg
     3241 cagacgttcg cgaaccacag gacgcgcatc gccaccgtgg cgtccccggc gccgcggaag
     3301 atcgcgttga cgagaaacag gaggacgatg acgacgtttc cgccgagcat cacggccatg
     3361 aaccccgagc ccgaggccac gacgtccggc cccgccccca tgaacgcgag gaggcgcggc
     3421 gcgaggagcg cccccgcgac gccgaggggc acggcgacga ggacgccgag ggcgatcgcc
     3481 tgcacggccg tccgggcggc gctctcgcgg tcgcgctcgc cgatgcgccg tgcgaccatc
     3541 gccgtcgccg ccatcgagag gccgatcgcg agcgagtaga ggatcgagag gatcgactcg
     3601 gtcaggccga ccgtcgcgac cgcggccgcg ccgagcttcg acacccagaa gacgtccacg
     3661 accgcgaaga ccgactcgag cgccatctcg aggaccatgg ggatcgcgag aaggacgacc
     3721 gcgcggccga ccggcccctc ggtgaagtcg ccgtgccctc cgcgcagcgc ctcgcgcagc
     3781 gtcgcgagga cgccgggggc cggcgggggg gcctccggag gggacatgag ggaagggtac
     3841 gcgtcctaga cgttgaactt gaagagcatc acgtcgccgt ccttcacgac gtagtcgcgc
     3901 ccttcctgcc gcacgacgcc ggcttccttc gccgccgcca tcgagccgtg cgtcacgagg
     3961 tcgtcgtagg cgatcgtctc ggccttgatg aagcccttct cgaagtccga gtggatgacg
     4021 ccggccgcct gcggggccgt gtcgcccgcg tgaatcgtcc acgcgcgcgc ttccttctcg
     4081 cccgcggtga agtacgtccg gaggcccagg aggtggtacg ccgcccggat gagccggccg
     4141 acgccggact cgtcgacgcc gtgcgacgcg aggtactccg ccgcgtcctc gggcgagagt
     4201 tccgcgaggt cggcctcgat cttcgcggag atcgcgaccg cctccgagcc gctgtgcgcc
     4261 gccgcccagt cgcgcacctt cttcacgagc ggaaggtcgt ccgcgtgcgc gaggtcggcc
     4321 tcggcgacgt tgcaggcgaa gagcgtcggc ttcatcgtga ggaggaagaa ggctcgcgcg
     4381 gcggccttct cctccgcggt gaggtcgagc gtgagggcgg gctttccccc gtcgaggtgc
     4441 gggaggacct tctcggcgac ggcgagctgg gccttggcct ccttctggcc gccgcgcgcg
     4501 agcttctcga ggcgctcctt ctgcttgccg agggactcga gatccgcgag gacgagttcc
     4561 gtcgcgaccg tctcgatgtc ggcgacggga tcgagagtct cggagacgtg gacgatgtcc
     4621 ttgtcctcga agcagcggac gacctgcacg acggcgtcga cctcgcggat gtgcgagagg
     4681 aacttgttgc cgaggccctc gcccttgctc gcgcccttga cgaggcccgc gatgtcgacg
     4741 aactcgaacg tggccgggat cttcaccggc gtcttcgcga tcctcgcgag cggctcgagg
     4801 cgcgcgtcgg gcaccgtgac gatgccgacg ttcggctcga tcgtgcagaa cgggtagttg
     4861 gccgcctcgg ccttgcgggt cttcgtgagg gcgttgaaga gcgtcgactt cccgacgttc
     4921 gggaggccga cgatgccggc tcggagcatg gaggtccttt cgggggtgag gcgccgcgcg
     4981 gacgcgggcg ccggggcgca gaggttagca tccgcgcatg aacgagacgc cgcgcctccg
     5041 ggtcttcacc ctcggcctcg ccctgctcgc cctcgccgcg gcgcccgcgg acggcgcgaa
     5101 gaagccctcg aagaagagcc cccacacgca gatcgtcgcg gtgtgccgcg tcgagacggc
     5161 gcccgccgag aagacgcaca agaacgggcg cacgttcctc gagttcgacg tgacgatcgt
     5221 gtcctacgag cgcgtgaagg gcgacgcggc cgccgacccg gccctcgcga tccggcagga
     5281 ccgccgcatc cacgtcgtcc acgacctctc gtgcggcggc gagcgtctcg ccctcgcgaa
     5341 gggcgacgcc gccgagatcc ggggcgagta cgtcgcgccg gacaacggca aggacctcat
     5401 ccacttcacg caccccgccg acggctcctg cgggacgaaa gggggccacc ccgacggcgg
     5461 catccggaaa ctcgacgcgg cggccctcaa gaaggcggct tgagcccgga cgacgccggc
     5521 cgcgcgggcg ccgggaggct cctggccgcc tgggacgcgt attcgcggga gttcgccgcg
     5581 atcacgcgcc gggcgccggt gcgcttttcg gcgcgcgact ggaggggcgt gcgcgcggac
     5641 gcgctcgagc gcctcgagct ctaccggaag atgctcgacc ccgccgtcga ggacctcggg
     5701 cgcatcctcg ggcccggcgt cgggaacccg gccgcctggt ccgggatgaa ggagcgattc
     5761 gcggcgctcg ttgccggccg gaaggacgtc gagctcgtgg agacgttctt caactcggcg
     5821 tcgcgccgca tcttccacac cgtcggcgtg gacgatcggg ccgagttcgt ccaccgcgag
     5881 ccgacgccgc tgcccgtcga ggccgcgggg cgtttcctcg cgtccttcga cgggcgcccc
     5941 gggcgcgagg ccgcggtccg cggcgcactc tccgtctccc cgttcgccgc gggctgggaa
     6001 gacctcacgc gcgacgccgc gctcgcgacg cgcgcgctcg acagggtcct cggcgcggcc
     6061 tggggttctc cggcgttcga cgccctcgac gttctgaggc ccgtgttctt ccggggcaag
     6121 ggcgcgtacc tcgtcgggcg cgtccgccgg gagggccgcg cgcgccccgt cgtcttcgcg
     6181 ctcctgaacc gcacgggccg cgtcgaggtg gacgccgtgc tctgcgacga ggacgaggcc
     6241 tccatcgtct tcagcttcac gcgctcgtac ttccacgtcg acgccgagca gccggccgct
     6301 ctcgtcgggt tcctcaagac gctcatgccg aagaagcccg tcggcgagct ctacaacgcc
     6361 ctcggcttca acaagcacgg caagacggag ctctaccgcg acctcctgcg ccacctcgcc
     6421 gcctccgacg accggttcgt cgaggcgccg ggggctcgcg ggatggtcat gctcgtcttc
     6481 acgctgccgt cctacgacgt cgtcttcaag gtcatccgcg actccttcgc gcccccgaag
     6541 acggcgagcc gcaaggacgt cctcgacaag taccgcctcg tcttccggca cacgcgcgcg
     6601 ggccggctcg ccgacgtcca ggagttcgag cacctctcct ttcccgtcgc ccgattcgac
     6661 gccccgctcc tcgagacgct cctcgcgggc gccgccgaaa cggtggaggt gcgcggggac
     6721 gaggtcgtcc tccgtcacct cttcacggag cggcgcctga cgccgctcga catcgcgctg
     6781 aaggaggcgt cgcccgcgca ggcgcgcgcc gcgatcctcg acttcggcga ggcgatccgc
     6841 gacctcgcca aggcgaacgt cttccccggc gacctcctcc cgaagaactt cggcctgacg
     6901 cgccacggcc gcgtcgtctt ctacgactac gacgagatcg acttcctgac ggacgtgaac
     6961 ttccgcgagc tgcccgcggg cgccgacgag gaggccggcg gggacgacgc gtcgttcttc
     7021 gtgggcgagc acgacgtctt ccccgaggag ttcctgatgt tcatgggcct gagagggccc
     7081 gcgcgcgagg cgttcctcga gcgccacgcg gaccttctcg gcgtccgctt ctggctcgag
     7141 gcgcagcgcc gggagcgtgc gggtgagatc gtggacgtct acccctaccc gctcgaggcg
     7201 cgtctcagga tcgactgatc agcgggcgcg cgcgccgcca cgcgccgtac gttcgcgggc
     7261 cgaccaggcc cacgagggcg aggcggcgca ggtgcttcgg cgacgggtgc agggccgccg
     7321 cgcgcttgag gagcggcagg acctcctcgc gcgcgccccc gctccccgac tccatcgcga
     7381 gcgcggcctg catcccgagc atcttcgcga gcgccttctt caccgcaccc tcgcggccgg
     7441 cgagcgcggg gtgcggcggg tcgaggagcc gctcgaggat cgggacgttc aggcgcacac
     7501 tctcgaggag gctcgccgac gcgttctctc cgtggcggcg gtaccggtag agcggccggt
     7561 cgagatagcc catccggtgc gcgagcgaga atcgcagcgc catgtccgtg tcggcggcat
     7621 agctgtccgt cgaataggga cccgcctcga gaagcgcggc gcgacgcacg acaggcgtcg
     7681 agctggcccc aagctcggaa gagagaaatc cttcgaacgt gaattcggct ccgggaagac
     7741 gccggcgcga gatttctccg gtgggggcgc cggaagcgtc gatgagaaga cgggcggtcg
     7801 aaacccaggg gatctctcta ttaccttcta agaaatcttc ggtctcttcg agatatcgcg
     7861 gttcgaggac gtcgtccgac tcgagaaagg cgatgacgtc cgccttcacg gccgcgacgc
     7921 ccatgttgta cgtcgagccg attccgccgc gtgcgttctc gagcacgcga atacggcctc
     7981 cgaaccctgc gaggacgtcg agcgtcccgt ccgtcgagcc gtcgtccacg acgacgacct
     8041 cgtcgggcgg ccgcgtctgc gagagcgcgc tctcgaccgc ctcgccgacc cagcgcgcgt
     8101 gctggaagga cgggatgacg acggcggccg tcgcccgggc cgtcacgggc gcgcgccgcg
     8161 caggcggctc ttgaagccgc ggaaggcggc g
//
LOCUS       DAOMFY010000169         8186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2729_length_8186_cov_6.991268, whole genome shotgun sequence.
ACCESSION   DAOMFY010000169 DAOMFY010000000
VERSION     DAOMFY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2729_length_8186_cov_6.991268"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLB01_13065"
     CDS             complement(<1..180)
                     /locus_tag="PLB01_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78277.1"
                     /translation="MTRRTFLATLLAFAVTARADAPRRLRRILRLRSGMVEEVPLEDY
                     VEQCYRQTVTGLNCYP"
     gene            224..1216
                     /locus_tag="PLB01_13070"
     CDS             224..1216
                     /locus_tag="PLB01_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ78278.1"
                     /translation="MRQRRAYDTRASDGSPGRAPVVAAILPAYRTASAIRAVIERIPA
                     SVTHVIVVDDASPDGAGSAASASGDPRVLVLSHDTNRGVGGAMKTGFTKALELGADVV
                     VKLDSDGQMDPSMIPALVAPLLEGRADLAKGNRFADLAVISAMPPLRRAGNLALSFLV
                     KAASGYWSLFDPCNGFLAVRCDLLKNVRQDRLADRYFFEISLLCESYRAEGVVLDVAM
                     SPRYGDETSSLSPTRSLFEFSGRLLARTLSRIGSTYFLRDFNVVSVLISAGVPLMTFG
                     VLWSAYHWYRSWVTNVVASTGTVMIGTLAIILAFQLLLQAVVLDVQNEPGRAPR"
     gene            complement(1229..1744)
                     /locus_tag="PLB01_13075"
     CDS             complement(1229..1744)
                     /locus_tag="PLB01_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ78279.1"
                     /translation="MSRGVEALYKKSDPEAAAKDFRKILAKNPEHYGATYQLAVALDR
                     GGRAAEARPLWEKSLAMAEAVKDAKTAETARQRLGVPAVAPEDAAMKAGLDALYEKKD
                     AGAAVAEFQKVLQLNPSHYGATFQLAKALDQAGKPAEARPYWGKALKMAEGYKDAKTV
                     ETARERLARRP"
     gene            1902..3566
                     /locus_tag="PLB01_13080"
     CDS             1902..3566
                     /locus_tag="PLB01_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78280.1"
                     /translation="MQSPWLRAALMVLVCAAIYAAGAALLGDWIIDDAGISYAYARNL
                     AAGHGLVSQPGRPPVEGFSNFLWVVLLTPLFLARVFDPVMVVKVLAGLLVLLSVWILQ
                     RTLRREPGGWAPALLATALVAGSSPIVIWTASGLENSLTLLLGVVLYDRLVWLPGRWE
                     LQAGILTALLAMNHPENLLYIAAGLLVCCGPLLMRRESIAEAARHVGRYVAGFALLFS
                     PFMAFRLATFGLPFPHTYYAKRRFPDAWASLAALFRHPADTARKLLDLLQGIAGPLGL
                     VAMIVTAAAALHLLRRRTVDRTFGVALAIQAVAIGAYLWMDEDWMGEYRFATLATAFS
                     LVSFVIAGFSILARPSGRGRLLVTAAYTGAAAFLLARGARRIVDFAQAPPAPFAEMAR
                     QHAFKFNVYADVLGLRHASLLLPDIGAALVYSDLTVYDAAGLCEPDVVRTLKGGTIYW
                     LDHHPAFYDWVFETVRPTFINTHDFFTHVTALERDPRFARDYAAVNEYRDEYVARMYG
                     WAAHSGDYVRRDALRSPADLEALRRSYEPLPPLRPPFEVLRARYPF"
     gene            3621..5639
                     /locus_tag="PLB01_13085"
     CDS             3621..5639
                     /locus_tag="PLB01_13085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ78281.1"
                     /translation="MKRPAPEPDGRGAGSSSAFLLTAAGCLALLFAAYANSLENAFHF
                     DDTHVVVGNLSIRNLANAGRFFTDVRTTSALPQNQAYRPLVTLSLAVDYALGRGLSPR
                     AFHLGQLFLLVSLGAALFFFYRRVMDRASPGPLNRFLALFAATLFCVHTVNTETMNLM
                     HARSEILSALGIVGGFLVYLGAPRLRRFQVHLAPMAAGALAKTPAVLLAPLLFVWEFL
                     VPGTEPNLSRPFGVRLRSALRAAAPALLAGLALFWFVEKWMAPPTLDYGGGNRIAYAQ
                     TQLWVWLHYLRLFVLPVGLSADTDLRLISVWYDSRVLAGGCVLAALAWLALRCARAPR
                     AWPVTFGLAWFAIGLLPTSSVIPLAEPLNEHRVFLPYIGLVLSAVWGARLLLSGRARQ
                     RIAIATLCLAVLAVLAAGTHVRNRTWRTRQSLWEDVTRKSPGNGRGWTNYGEALMANG
                     DYARARECFERAAPLEPNYWALETNRGVVEEALGNTATAESHFRRALDLGPGQPDVHV
                     LYARFLARVGRGPQAVQHLETALRLSPGAELARSLLMDLRAAIGEADGAAALAREALE
                     ADPANARARDYLTSGFPSDLGASPAACRQTGSALIRRGDFIAAALAYRAALRLDPADA
                     DTLNDLGWTMGKLGYFDRAVPLLERALALRGDFELARNNLAWARSGLK"
     gene            5636..6541
                     /locus_tag="PLB01_13090"
     CDS             5636..6541
                     /locus_tag="PLB01_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ78282.1"
                     /translation="MSDANACPVCGSGRPAHPRILPGLRIDSCGACGHREARHGAAKP
                     GSDYHDQYDAGPFLEALRATRVRQARRLIEVLRRHVPDASGVVDYGAGRGWFLEACRA
                     AGIERLAGVDTSQVSVDGLTRSGIEVHRLSTDEDAASALSRLSFRPRVLSLLDVIEHF
                     PPDRLESRLRSIVGACGRGLELVIVKVPVPGLLYAGASALARVGFSGPFLQLYQAGTW
                     PPHFSYFSLASARLALAAAGLSVIESFGDADFEPAFLGQRIGATGPISTALARVGGEA
                     LGAAIGVTGRFDSVVLLARLASALP"
     gene            complement(6600..6977)
                     /locus_tag="PLB01_13095"
     CDS             complement(6600..6977)
                     /locus_tag="PLB01_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78283.1"
                     /translation="MSGTPIIGGTSGTANMVSGRYMGMFAGNQSGTERSVQNIMPTAG
                     TVKNFYVFVENPPGGTTSWTLTLRKNGVDTAVTCTVTGAGQTCFDTVNTAVFAAGDLI
                     SVRESSSGSPLNSAGQWTAVFAP"
BASE COUNT         1095 a         3043 c         2600 g         1448 t
ORIGIN      
        1 cgggtagcag ttcagcccgg tgaccgtctg gcggtagcac tgctcgacgt agtcttcgag
       61 cgggacctct tcgaccatcc ccgaccggag ccgcaggatc cggcgcaggc gtcgtggggc
      121 gtcggcgcgc gcggtcacgg caaacgccaa gagcgtcgcg aggaaggttc gccgggtcac
      181 gcgctcgcga ttctagggcc tggcccgcgg tatcctctcc gaaatgcgcc agcgacgggc
      241 ctatgacacc cgggcttccg acggctcgcc gggacgcgcg ccggtcgtcg cggcgattct
      301 tcctgcctat cgaaccgcgt ccgcgatccg cgccgtgatc gagcggatcc ccgcgagcgt
      361 cacgcatgtc atcgtcgtgg acgacgcctc gccggacggc gccggctcgg ccgcctcggc
      421 gagcggagat ccgcgggtcc tcgtcctctc gcacgacacg aatcgcggcg tcgggggcgc
      481 aatgaagacc ggattcacga aggctctcga gctcggtgcg gacgtcgtcg tcaagctcga
      541 cagcgatggc cagatggacc ccagcatgat tccggccctc gtcgcgcctc ttctcgaggg
      601 gcgagccgac ctcgcgaagg gcaaccggtt cgcggacctc gccgtgataa gcgcgatgcc
      661 gccgctccgg agggccggca acctcgccct gtcctttctc gtgaaagcag catcgggcta
      721 ctggagcctt ttcgatccct gcaacgggtt cctcgccgtc cgatgcgacc tcctcaagaa
      781 cgttcgccaa gaccggctcg cggaccggta cttcttcgag atcagcctgc tgtgcgagtc
      841 gtatcgcgcc gaaggagtcg tcctcgacgt ggcgatgagc ccccgctacg gcgacgagac
      901 cagttcactg agcccgaccc gttctctctt cgagttctcg gggcggctcc tcgcgcggac
      961 gctgagccgc atcggttcca cgtacttcct gcgcgacttc aacgtcgtgt ccgtcttgat
     1021 ctcggccggc gtgcccctca tgacgttcgg cgtcctctgg tcggcgtacc actggtaccg
     1081 ctcctgggtc accaacgtgg tggcgtccac gggcacggtc atgatcggta cgctcgcgat
     1141 catcctggcg tttcagctcc tcctgcaggc cgtcgttctc gacgtgcaga acgagcccgg
     1201 gcgcgcgccg cgctgagctg actcttcgtc aggggcgcct tgcgagccgc tctctggccg
     1261 tttcgaccgt ctttgcgtcc ttgtagccct ccgccatctt gagggctttc ccccagtacg
     1321 ggcgcgcctc cgcgggtttc cctgcctggt caagagcctt ggcgagctgg aacgtggcgc
     1381 cgtagtggct cgggttgagc tgcaggactt tctggaattc ggcgacggcg gcgcccgcgt
     1441 ccttcttctc gtagagggcg tcgagtcccg ccttcatcgc cgcatcctct ggcgcgaccg
     1501 ccggcacgcc gagccgctgg cgcgccgtct cggccgtctt cgcgtccttg acggcctcgg
     1561 ccatcgcgag cgacttttcc cacagaggcc ttgcttccgc cgccctgcct ccacggtcca
     1621 gcgcaaccgc aagctggtac gtggcgccgt agtgctcggg gttcttcgcg aggatcttgc
     1681 ggaagtcctt cgcggccgcc tccgggtcgc tcttcttgta cagggcctcg acgccccgac
     1741 tcatcaaggc atccggcgtt tcctccgccc gggagcaggc caacgccgca ccgaccacga
     1801 ccaatgcgag gaaccggaag aaccttactc tcatattcct tccagtatag ggcttcgtcc
     1861 ggccctcgtc ggccgcgcga acacacccta aaatcgcaat cgtgcaatcc ccatggctcc
     1921 gcgcggcctt gatggtgctc gtctgcgcag cgatctacgc ggcgggagcc gcgctcctcg
     1981 gtgactggat catcgacgac gcgggcatct cctacgcgta cgcgagaaac ctcgccgcgg
     2041 gccatggact cgtctcccag ccggggcgtc ccccggtgga agggttcagc aacttcctgt
     2101 gggtggtctt gctgaccccg ttgtttctcg cacgcgtctt cgatcccgtc atggtcgtca
     2161 aagtgctggc ggggctcctc gtcctgctga gcgtctggat cctgcaaaga acgctgcgtc
     2221 gggagcctgg cgggtgggcg ccggccctgc tcgcaacggc gctcgtcgcg gggtcctccc
     2281 ccatcgtgat ctggacggct tcgggactcg agaacagcct gacgctcctg ctcggggtgg
     2341 tcctgtacga ccgcctcgtg tggttgcccg ggagatggga gctccaggca gggatcctca
     2401 ccgctcttct ggcgatgaac caccccgaga acctgctcta catcgcggcc ggccttctcg
     2461 tctgctgtgg cccgctcctg atgcggcgcg agtccatcgc cgaggcggcg cgccacgttg
     2521 gacgctacgt ggcggggttc gccctcctgt tctctccgtt catggccttc cgcctcgcga
     2581 cgttcggcct gcccttcccc cacacgtact acgcgaagcg acggttcccc gacgcctggg
     2641 cttcgctcgc ggctctgttc cggcatcccg cggacacggc gaggaagctc ctcgatctcc
     2701 tgcaggggat cgccgggccg ctgggtctcg tggcgatgat cgtgactgcg gccgctgcgc
     2761 ttcatctcct gcggcggcgg accgtggacc gtacgttcgg tgtggccctg gccattcagg
     2821 ccgtcgcgat cggggcctac ctctggatgg acgaggactg gatgggcgag taccgcttcg
     2881 cgactctcgc cacggcattc tccctggtct ctttcgtgat cgcgggcttc tcgatcctcg
     2941 cccgaccctc cggccgcggc cgcctgctcg taaccgccgc ctacacgggc gcggcggcgt
     3001 ttcttctcgc gaggggcgcc cggcgcatcg tcgatttcgc tcaggccccg ccggcgccgt
     3061 tcgcggagat ggcgcgccag cacgccttca agttcaacgt ctacgcggac gtcctcggtc
     3121 ttcggcacgc ctcgctgctc ctccccgaca tcggcgccgc cctcgtgtac tcggacctca
     3181 ccgtgtacga cgcggccggt ctctgcgagc cggacgtcgt gcggacgctg aagggcggaa
     3241 cgatctactg gctcgaccat cacccggctt tctacgactg ggtcttcgaa acggtccgcc
     3301 ccaccttcat caacacgcac gacttcttca cccatgtcac ggcgctcgag cgcgaccctc
     3361 gattcgcgcg cgactacgcg gcggtcaacg aataccgcga cgaatacgtc gcgcgcatgt
     3421 acggctgggc ggcgcactcg ggcgactacg tgcgccggga cgcgctccgc agccccgccg
     3481 acctcgaggc gttacggcgc agctacgagc cgctcccgcc gctccggccc cctttcgagg
     3541 tcctccgggc gcggtatcct ttctgagatg caccgacgtc gagcgcggga attccggccg
     3601 atcgccggcc gcgtccggag gtgaagcgcc ccgcgcccga gccggacggc cgaggcgccg
     3661 gctcttcgtc cgcgtttctc ctcacggccg ccggctgtct ggcgcttctc tttgcggcgt
     3721 acgccaactc cctcgaaaac gcgttccact tcgacgacac tcacgtcgtc gtcggcaacc
     3781 tctccattcg aaatctcgcg aacgccgggc ggttcttcac cgacgtccgt acgacgagcg
     3841 cccttccaca gaatcaggcc taccgccccc tcgtcacgct cagtctcgcg gtcgattacg
     3901 ccctcggccg cggcctgtcc ccacgggcgt ttcacctcgg ccagctgttc ctgctcgtct
     3961 ctctcggcgc cgcccttttc ttcttctacc ggcgggtgat ggaccgcgca tctcccgggc
     4021 ctctgaacag gttcctcgcc ctcttcgcgg ccacgctctt ctgcgtgcac acggtgaaca
     4081 ccgagacgat gaacctcatg catgcgcgct cggagatcct cagcgcgctc ggcatcgtcg
     4141 gcgggttcct cgtctacctc ggcgctcccc gcttgcgtcg tttccaggtg catctcgcgc
     4201 ccatggccgc gggggccctc gccaagacac cggccgtcct gctcgccccg ctgctgttcg
     4261 tctgggagtt cctcgtcccg ggaacggaac cgaatctatc ccgcccgttc ggcgtccgcc
     4321 tccggtcggc ccttcgcgcc gcggcgccgg cactcctcgc gggcctcgcg ctcttctggt
     4381 tcgtcgagaa gtggatggca ccgccgaccc tcgactacgg cggcggaaac cggatcgcat
     4441 acgcccagac gcagctctgg gtctggcttc actaccttcg actgttcgtc ctcccggtcg
     4501 gcctgtcggc ggacacggac ctcaggctga tctcggtctg gtacgactcc cgcgtcctgg
     4561 ccggcggctg cgtgctggcc gctctcgcat ggctggctct tcgctgcgcg cgggcgccgc
     4621 gggcgtggcc cgtcacgttc ggcctcgcgt ggttcgcgat cggtctgctt ccgacctcga
     4681 gcgttattcc cctggccgag cctctcaacg agcaccgcgt cttccttccc tacatcggcc
     4741 tcgttctgtc ggctgtctgg ggcgcccgac ttctcctctc cggccgcgcc cgacagagaa
     4801 tcgcgatcgc gaccctctgc ctcgcggtgc tcgcggtgct cgcagcgggg actcacgttc
     4861 gcaaccgaac gtggaggacg cgtcaatcgc tctgggagga cgtgacgagg aagagccccg
     4921 ggaacggccg cggatggacg aactacggcg aggccctgat ggcgaacggc gactacgcgc
     4981 gtgcccgcga gtgcttcgag cgcgcggcgc cgctcgagcc gaattactgg gctctcgaga
     5041 ccaaccgcgg cgtcgtcgaa gaggccctcg gcaatacggc gacggccgag tcgcacttcc
     5101 ggcgagcgct cgacctcggc cccggacagc ccgacgtgca cgtcctctat gcccggttcc
     5161 tcgcccgcgt cggccgcgga ccgcaagccg tgcagcatct cgaaacggcc ctccgcctca
     5221 gcccgggcgc cgagctcgcg cgaagcctcc tgatggacct tcgggccgcg atcggcgagg
     5281 ccgacggcgc ggcggcgctc gcccgggaag cgctcgaggc cgatccggcc aatgcccgcg
     5341 cccgggatta cctcacgagc gggtttcctt ccgacctcgg cgcaagcccc gccgcctgcc
     5401 gccagaccgg atcggcgctg atccgacggg gcgatttcat tgccgccgcg ctcgcgtacc
     5461 gtgcggcgct ccgcctcgac cccgcggatg cggacacact gaacgacctc ggctggacga
     5521 tgggaaagct cggttatttc gaccgggccg tcccgctgct cgaaagagct ctcgcgctcc
     5581 ggggagactt cgaactcgcg cgcaacaacc tcgcctgggc gaggagcggg ctcaagtgag
     5641 cgacgccaac gcgtgccccg tctgcggcag cgggcgcccc gcgcacccgc gaattctgcc
     5701 gggcctccgg atcgactcgt gcggcgcctg cggccaccgg gaggcccgtc acggggccgc
     5761 gaaaccggga tccgactacc acgaccagta cgacgccggc ccgtttctcg aagcgctgag
     5821 ggcgaccaga gtccgtcagg cgcggcgcct gatcgaagtc ctgcgtcggc acgtccccga
     5881 cgcctcggga gtcgtcgact acggagccgg ccggggctgg ttcctcgagg cgtgccgtgc
     5941 cgccggaatc gagcggctcg cgggcgtcga tacctcacag gtttccgtcg acggcctcac
     6001 gagatccggg atcgaggtcc atcggctttc gacggatgaa gacgcggcat cggccctgtc
     6061 acggctttcc ttccggcccc gtgtcctcag cctgctcgac gtgatcgaac acttcccgcc
     6121 cgaccggctc gagtcgcgcc tgcgaagcat cgtcggtgcg tgcggccgcg ggctcgagct
     6181 cgtgatcgtg aaggtccccg tgcccggcct cctgtacgcc ggcgcgagtg cactcgcccg
     6241 ggtcggcttt tccgggcctt ttctccagct ctaccaggcc gggacgtggc ctccgcactt
     6301 cagctatttc tcgctcgctt ccgcccgact ggcgctcgcg gcggccgggc tttcggtgat
     6361 cgagagtttc ggggatgccg acttcgagcc ggcgtttctc gggcagcgca tcggcgcgac
     6421 cggcccgatc tcgacggccc tggcgcgcgt cggaggcgaa gctctcggcg ccgcgatcgg
     6481 cgtgacggga cgattcgact ccgtcgtcct gctggcacgc ctcgcgtccg cgcttccctg
     6541 aaaggaaacg ggcgcgggtt caacccgcgc ccgccgaaat cccgaatcgc gagcggtgcc
     6601 tacggggcga acaccgccgt ccactggcct gcggagttca acggcgatcc gctggaagac
     6661 tcccgcaccg aaatgaggtc accggccgcg aatacagccg tgttcaccgt gtcgaaacag
     6721 gtctggccgg cgcccgtcac cgtgcaggtc accgcggtat ccacgccgtt cttccggagc
     6781 gtgagcgtcc aggacgtcgt gccccccgga gggttttcga cgaagacata gaagtttttc
     6841 acggtgcccg cggtgggcat gatgttctga accgaacgct ccgtgccgct ctggttgccc
     6901 gcgaacatgc ccatgtaacg gccgctgacc atattcgctg tcccgctcgt tccgccgatg
     6961 atcggcgtgc cggacacggg gccggtcgga ccggtcgcac ctgtagcacc cgtgggacca
     7021 acggcgccgg tcaaaccagt cgggccggta ggaccaacgg cgccagtcgc accggttgcg
     7081 ccggttgagc ccgtcgctcc ggtagcgcca gtagcacccg tcgctccagt tggaccaact
     7141 gcgccagttg ctcccgtcga gcccgtggct cccgttgcgc cggtcgaacc cgtggctccg
     7201 gtggctcccg tcggcccagt cggaccaact gcgccggttg aacctgtcgc tcccgtcggt
     7261 ccagtcggac caacggcacc agtcgcgccg gtcgagcccg tggctccggt agcaccagta
     7321 gctccagtcg gcccggtcgg accaactgcg ccagtcgctc cagtcgagcc agtcgctccc
     7381 gtcggaccaa cagcgcccgt tgcgcccgtc ggaccaacgg cgccagtcgc accggtcgaa
     7441 cccgtggcgc cagttgcgcc cgttgcgccg actgcacccg tggcaccagt ggctcccgtc
     7501 ggaccagttg ggccaacggc accagttgcg ccggtcgaac ccgtggcacc agttgcgccg
     7561 gttgcaccag tggctcccgt cggcccagtc ggaccaactg caccagttgc gccggtcgag
     7621 cccgtggctc cggtagcacc ggtggcacca gtagctccag tcggcccggt cgggccagct
     7681 gcacccgtcg cgcccgttgc gccggccgca ccagtggctc ccgtcggacc agtcggacca
     7741 acagcgccgg ttgctccagt cgcgcccgta gcgcccgtag gaccagtcgg accaactgcg
     7801 ccagtggctc cagtcgagcc cgtggctcca gttgcaccgg tggccccagt agctccagtc
     7861 ggcccggtcg gaccaactgc gcccgtcgaa ccagtcggac caacggcacc agtcgctccg
     7921 gtcgcacccg tggctcccgt tgcgccggtc gcacccgttg cgccagtagg accggtcgga
     7981 ccaactgcgc cagtggcgcc cgtcgaacca gtactacccg tcgctccagt cgcgcccgtg
     8041 gctcccgttg ctccggttgc gccagtggcg ccagtaggac cagtcggacc gacagcgcca
     8101 gtggcaccgg tcgaaccagt actacccgtg actccggtgg cacccgttgc acccgtcgct
     8161 ccagtcggac caacagcacc ggttgc
//
LOCUS       DAOMFY010000170         8181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2730_length_8181_cov_7.094019, whole genome shotgun sequence.
ACCESSION   DAOMFY010000170 DAOMFY010000000
VERSION     DAOMFY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2730_length_8181_cov_7.094019"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLB01_13100"
     CDS             complement(<1..181)
                     /locus_tag="PLB01_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78284.1"
                     /translation="MSENEPKERVLTTPEGEAPGGEPEGDFEPEAGDDAGDGPEGPEG
                     GEGAAAGEGGAPGSFD"
     gene            482..3052
                     /locus_tag="PLB01_13105"
     CDS             482..3052
                     /locus_tag="PLB01_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78285.1"
                     /translation="MTIPHPLPRPSRFLRLLPATLLAAALGARAQTPTPTPTPTPTPT
                     PTPAVTALTANSNLYTFPNAPNIDGANLSLDPDGSVWSVSAADNKISKLSPDRTTVTR
                     WTFAADTAPSSLLKDSDGTFWITEMGGFNVGHFNPADGTLTRWPDAYRRPTALAKRPD
                     GKFWLPETGGYMAVFDPAAPSFTYWVTPGVFYLSYPWVDDDGSVWACDFIYNSIIHWA
                     PDMSTARVWPLPLDIFVSAPSKIIRLGGKLWISIYYSSQIARFDETTGKIDFFDTGAG
                     TSPYDLHEYRGRILYSDQAGQIGFLDPTGSVPVTKTLTPTDVKPLVQAVYTTAASVST
                     LSSVDEPVTTTYLGSISGVQGPDGGIVYPANGSAVWGMVVDEKRARIYFGTNASVGAL
                     SAPPYVQADELYFPVASAGNPATGTTTQIVTWNRGTPDAQGATHTMNQVERLLPEGWI
                     AGWSPAAQQAIDAGKLLVQSDAVGTDMKAPGALGSIRLLSDRGADTFAWSRIAVPAPG
                     GGTLGSATNAVLGTAAIAAGDSGFVFTPADPAFRTHAGIFIVQDATGTVSVVDAAGQT
                     RVTFPFDWPSGFRAQGSTIFEVFGLPALPSARIVCTVTTGSVLLFGTAYDPVSGDAVR
                     LEPFRSADVGLWPVLPGFVRGGGLAGTLQLFNPGTTTAHVNLSFRAAQAPGAPAAPGQ
                     AIGSVTVPAGAVVSVDLGAGAAAQGALDLSSDQNVAAFATYRRPLAGGGTAGYGVAAQ
                     QIGAAIPAGSRGVFVSSTVNDAFTSTLQLVNTGFEPAVVTVAFTNADGTDTGGAQAVT
                     LPAQGVVNLPGWPAGQTTDAGRVAVTPADGSPAVLAILARRDRVSQDTDAISPFVVPK
                     "
     gene            3071..3895
                     /locus_tag="PLB01_13110"
     CDS             3071..3895
                     /locus_tag="PLB01_13110"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HQQ78286.1"
                     /translation="MTRRGFRAAAAVAAAVLAAIAGTPAAAQDAPGFPTTVGNRFSQT
                     STPLVNDKGTFEAVFTHRFNQNVKDAGGDSLFGLDSGASIGIGVEYVPVKNLAVQIYR
                     VNNYADYEFALKATLLRPTVKLPLGVGLRGGLDWRTAPYAPKETSWFGQAIVSYTIAD
                     RVTLAAAPSYAQNTQFQQDVWNVPLIAQVKITKTIALIGEYVPKKDYVPDSVGQWSVA
                     LEKQVFNHRFALWMGNSQATTVDQYVGGDYNGGVTDRNIRIGFNLSRAWDLFPAAK"
     gene            complement(3900..5591)
                     /locus_tag="PLB01_13115"
     CDS             complement(3900..5591)
                     /locus_tag="PLB01_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HQQ78287.1"
                     /translation="MRRPHRRLRTTPLFLLSLLVFTFIGSSSSAAADRVVVTILQTTD
                     LHGHLLPWDYLRAQPADDGLARVATRVEAIRRESPNVLLLDAGDTIQGTPIEFLHAKD
                     PSKGPDPMAEAMSAMRYDAMSVGNHEFNFGLAVLRKAQKESSFPWLSSNTRNVADGSA
                     AFPEYVVRTVGGIRIGILGLTTPNIPNWEPARNRPGLRWEDPVDAAKRLVPVLRGKEK
                     CSFVVALIHSGPEVDLKTGEPDGTEDENRVAALSKVPGIDLLLTGHTHRRIPLTRLNG
                     VALIQPGRWGDVLARVDVVFEKSGKSVKVADLTAALLPSDASVATDATVAAIAAPHDA
                     AARAYMDTVVATAEEDFPGARARLEDTALLDFVNDTMREATGADLSMTSLLPFRFEGF
                     RKGPIKVRNVYTLYPYENQLVEIEVDGATLKACLEHAAEFYGAAQWEDGKLVLKPKEN
                     MIPYNFDVVQGAAYRIDPTAPVGSRVKDLAYKGRPVQPSDRFTLAVNAYRAQGSGGYK
                     ALKGAKVVKEFPEEIRELLIERLKKLGTVRPQTDRNWVVAPDAAWGPEPPRRPAP"
     gene            5624..6481
                     /gene="nth"
                     /locus_tag="PLB01_13120"
     CDS             5624..6481
                     /gene="nth"
                     /locus_tag="PLB01_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQQ78288.1"
                     /translation="MPSKKSPPAPAPVPETLARLEHLYGDAGCSLDFGSPLQLLTATI
                     LSAQCTDARVNMVTPEVFKRFPDAAALAAAPPGALEDVIRSTGFFNAKAKSLRGMAKA
                     LVEKHGGEVPRTMAELHALPGVGRKTANVVLGNVWNTPEGVVVDTHVGRLARRLGWTK
                     NADPVRVEQDLNERIPQERWTWISHALILHGRRICASRRPKCDLCTLADICPKVGVDE
                     KKKKQETERKNKRKTKISLKVKRAGATGAAKRAAAAKAPHAPSKKILSFSSSPSSARP
                     SAARKGPRR"
     gene            6478..7080
                     /locus_tag="PLB01_13125"
     CDS             6478..7080
                     /locus_tag="PLB01_13125"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HQQ78289.1"
                     /translation="MTPPGIGSIEVICGSMFSGKSEELIRRLTRAQIARRRVQAFKPV
                     LDNRYAEMEVVSHGGLRLVAVAVTTSAEVLARTDARTEVVGIDEAQFFDAAIVEVVSR
                     LADLGKRVIIAGLDQDYLGRPFEPMPALMAAAEEVTKTRAICVRCGQPASRTQRLVES
                     TERVVVGAAGLYEARCRRCFEPGVASSEGKLPFPDGSSTD"
     gene            complement(7395..>8181)
                     /locus_tag="PLB01_13130"
     CDS             complement(7395..>8181)
                     /locus_tag="PLB01_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQQ78290.1"
                     /translation="AKFRAARTLWARLVGERFGVTDPRAAALRFHTQTAGSTLTAQQP
                     EVNVVRVAIQALAAVLGGTQSLHTNGKDEALSLPTEDSALLALRTQQVIAHETGVARV
                     ADPLGGSWAIEALTAGIVAEAGALIARIDGMGGALKAIEAGFYQREIQDAAYRAQRAI
                     EDKEQIVVGVNEFTVGEERPIPTLVIDPAVETDQVARLKALRERRDAAAADRLRKGVA
                     DAAREGRNLLPPIVEAVSGLVTLGEVVEALRSVFGEHRESAGW"
BASE COUNT         1217 a         2964 c         2743 g         1257 t
ORIGIN      
        1 ggtcgaacga gccgggcgct ccgccctctc cggcagcggc gccttcgccg ccctccggac
       61 cctcggggcc gtcgcccgcg tcgtcgccgg cttccggctc gaaatcgccc tcgggctcgc
      121 cgcccggcgc ttcgccctcc ggcgtcgtca ggacgcgctc cttgggttcg ttttcgctca
      181 acctgtcctc cggggttggc cctgtcagga gccaacgggc ttcctggcct gcggtcgggg
      241 cccgcgtcgc ccgggcggcg cacgggatcc cggatcgtca ggtcctgcgt tgccgggcgg
      301 cggcgggatt ggacccggcc cttcggctcc gcatccggag cccgctggcc caccggatcg
      361 aggccgccct gcgacgacgt ttcagatgct acgcccattc ggaagcgtcc gtcaatcgcc
      421 ctcttctttc gacggcacgg gcggaacggg ccgtgcatcc gccgccctta caatgcgcgc
      481 cgtgacaatt ccgcaccccc tcccccgtcc gtcccgtttc ctccgcctcc tgcccgccac
      541 ccttctcgcg gccgcactcg gtgcgcgcgc tcagaccccg accccgacgc cgactccaac
      601 gccaaccccc acacccacgc ccgccgtcac ggcgctcacg gcgaactcga atctctacac
      661 gttcccgaac gccccgaaca tcgacggcgc gaacctctcg ctcgatcccg acggctcggt
      721 ctggagcgtc tcggccgccg acaacaagat ctcgaagctc tcgcccgatc ggacgaccgt
      781 cacgcgctgg acgttcgccg ccgacacggc gccgagctcg ctcctgaaag acagcgacgg
      841 gacgttctgg atcaccgaga tggggggatt caacgtcgga catttcaacc cggcggacgg
      901 cacgctcacc cggtggccgg acgcgtatcg ccggccgacg gcgctcgcca agcggccgga
      961 cggaaagttc tggctgcccg agacgggcgg ctacatggcg gtcttcgacc ctgccgcccc
     1021 gtccttcacc tattgggtca ccccgggcgt gttctacctc tcctacccgt gggtcgacga
     1081 cgacggctcc gtctgggcgt gcgacttcat ctacaactcg atcatccact gggcgccgga
     1141 catgtcgacc gcgcgagtct ggccgcttcc cctggacatc ttcgtcagcg ccccctcgaa
     1201 gatcatccgg ctcggcggga agctctggat ctcgatctac tactcctcgc agatcgcacg
     1261 gttcgacgag acgaccggaa agatcgactt cttcgacacc ggcgcgggca cgagccctta
     1321 cgacctgcac gagtaccggg gccggatcct ctactcggac caggcgggcc agatcgggtt
     1381 cctggacccg acgggttccg tgcccgtcac caagaccctc acgcccacgg acgtgaagcc
     1441 cctggtccag gcggtctaca cgaccgcggc cagcgtctcc acgctgtcct ccgtcgacga
     1501 acccgtcacg acgacgtacc tcggcagtat ctcgggcgtg cagggccccg acggcggcat
     1561 cgtctatccg gccaacggca gcgccgtgtg gggaatggtc gtcgacgaga aacgggcccg
     1621 catctacttc ggcacgaacg cgagcgtcgg ggcgctttcg gcgcccccct acgttcaggc
     1681 cgacgagctt tatttcccgg tcgcgtcggc gggaaatccc gcgaccggca cgacgacgca
     1741 gatcgtcacc tggaatcgcg ggacccccga cgcgcagggg gccacccaca cgatgaacca
     1801 ggtcgagcgc ctcctccccg aggggtggat cgccggctgg tcgcccgcgg cgcaacaggc
     1861 gatcgacgcc ggcaagctgc tcgtccagtc cgacgcggtc ggaacggaca tgaaggcccc
     1921 cggcgctctc ggatcgatcc gtctcctctc ggaccggggc gccgacacgt tcgcgtggtc
     1981 gcggatcgcc gtgcccgcac cgggcggggg gacgctcggc tccgcgacga acgcggtcct
     2041 cgggactgcg gcgatcgccg ccggcgactc gggcttcgtc ttcaccccgg cggacccggc
     2101 cttcaggacg cacgcgggca tcttcatcgt gcaggacgcg accggcacgg tctccgtcgt
     2161 cgacgccgcc ggacagacgc gcgtgacgtt cccgttcgac tggccttccg gattccgcgc
     2221 ccagggctcg acgatcttcg aggtcttcgg cctccccgcg ctgccttcgg cccggatcgt
     2281 ctgcacggtc acgaccggca gcgtcctgct cttcgggacg gcctacgacc cggtcagcgg
     2341 cgacgccgtc cgcctcgaac cgttccgctc ggccgacgtc gggctctggc ccgtcctgcc
     2401 cggattcgtg cgcggaggcg ggctcgcggg aacgctccag ctcttcaatc cggggacgac
     2461 gaccgcgcac gtgaacctct cgttccgcgc cgcgcaggcg cccggagcgc ctgcggctcc
     2521 cggccaggcg atcggcagcg tgacggttcc ggcaggcgcc gtcgtgtccg tcgatctcgg
     2581 tgcgggcgcc gccgcgcagg gcgcgctcga cctctcctcg gaccaaaacg tcgcggcgtt
     2641 cgcgacctac cggcgtcccc tcgcgggtgg aggcacggcg gggtacggcg tcgcggcgca
     2701 gcagatcggc gccgcgattc ccgcgggctc gcgcggggtt ttcgtctcgt cgaccgtgaa
     2761 cgacgcgttc acgtccaccc tgcagctcgt gaacacggga ttcgaacccg ccgtcgtcac
     2821 cgtggcgttc acgaacgcgg acggcacgga caccggaggc gcccaggccg tcactcttcc
     2881 cgcgcagggc gtcgtcaatc ttcccgggtg gccggccggc cagacgaccg acgcggggcg
     2941 cgtggccgtc acgccggcgg acgggagccc ggccgtcctc gcgatcctcg cgcgacgcga
     3001 tcgcgtgagc caggacaccg acgccatttc ccccttcgtg gtacccaagt aagcgcgagg
     3061 agggcactcc atgacgcgac gtggatttcg tgcggcggcc gccgtggctg ccgcggttct
     3121 ggccgcaatc gcgggcacgc cagccgccgc gcaggacgca ccgggcttcc cgacgacggt
     3181 gggcaaccgc ttctcccaga cctcgacgcc gctcgtgaac gacaagggca cgttcgaggc
     3241 cgtcttcacg caccgcttca accagaacgt gaaggacgcc gggggcgaca gtctcttcgg
     3301 cctggactcg ggagcctcga tcgggatcgg agtcgagtac gtccccgtga agaacctcgc
     3361 cgtgcagatc taccgcgtga acaactacgc ggactacgag ttcgcgctca aggcgacgct
     3421 cctccgcccg accgtcaagc tcccgctcgg ggtcgggctc cgcggcggcc tcgactggcg
     3481 gacggcgcct tacgccccga aggagacgag ctggttcgga caggcgatcg tttcgtacac
     3541 gatcgcggac cgcgtgacgc tcgcggccgc gccgagctac gcccagaaca cccagttcca
     3601 gcaggacgtc tggaacgtcc ctctcatcgc gcaggtcaag atcacgaaga cgatcgccct
     3661 catcggcgaa tacgtcccga agaaggacta cgtcccggac tccgtgggcc aatggtccgt
     3721 cgcgctcgag aagcaggtct tcaaccacag gttcgcgctg tggatgggga actcgcaggc
     3781 gacgacggtc gaccagtacg tcggcggcga ctacaacggc ggcgtcacgg accgcaacat
     3841 caggatcggc ttcaacctga gccgcgcctg ggacctgttc ccggcggcga agtagcggct
     3901 tacggtgccg ggcgccgcgg tggctcgggc ccccaggcgg cgtccggcgc gacgacccag
     3961 ttgcggtccg tctgcgggcg cacggtgccg agcttcttca gccgctcgat gaggagctcg
     4021 cggatctctt cggggaattc cttgacgacc ttcgcgccct tgagcgcctt gtagccgccc
     4081 gagccctggg cgcggtacgc gttcacggcc agcgtgaacc ggtccgacgg ctggacggga
     4141 cgccccttgt aggcaaggtc cttcacgcgc gagccgacgg gcgccgtcgg gtcgatgcgg
     4201 tacgccgcgc cctgcacgac gtcgaagttg tacgggatca tgttttcctt gggcttcagg
     4261 acgagcttcc cgtcctccca ctgcgccgcg ccgtagaact cggccgcgtg ctcgaggcag
     4321 gccttgagcg tggccccgtc cacctcgatc tcgacgagct ggttctcgta cgggtagagc
     4381 gtgtagacgt tgcggacctt gatcgggccc ttccggaagc cctcgaaccg gaacggcagg
     4441 agcgacgtca tcgagaggtc cgcccccgtc gcctcgcgca tcgtgtcgtt cacgaagtcg
     4501 aggagggccg tgtcctccag gcgggcgcgc gcgcccggga agtcctcttc ggcggtcgcg
     4561 acgaccgtgt ccatgtacgc acgcgccgcg gcgtcgtgcg gcgccgcgat cgcggcgacc
     4621 gttgcgtccg ttgcgacgga cgcgtccgag ggcaggaggg ccgccgtcag gtcggccact
     4681 ttcacgctct tcccggactt ctcgaagacg acatccacgc gcgcgaggac gtcgccccag
     4741 cggccgggct ggatcagcgc gacgccgttc agccgcgtga gcgggatgcg gcggtgcgtg
     4801 tgcccggtga ggaggaggtc gatcccggga actttcgaga gggccgcgac gcggttctcg
     4861 tcttcggtcc cgtccggctc gcccgtcttc aggtccacct cggggccgct gtggatcagc
     4921 gcgacgacga aggagcactt ttctttcccg cgcaggacgg gcacgaggcg cttcgccgcg
     4981 tccacggggt cctcccagcg gaggccgggg cggtttcgcg ccggctccca gttcgggatg
     5041 ttcggcgtcg tgaggccgag gatgccgatg cggatcccgc cgaccgtcct gacgacgtac
     5101 tcgggaaacg cggccgagcc gtccgcgacg ttgcgcgtgt tggacgacag ccacggaaac
     5161 gacgactcct tctgcgcctt gcgcaggacc gcgaggccga agttgaactc gtggttcccg
     5221 accgacatcg cgtcgtaccg catggcgctc atcgcctcgg ccatggggtc gggccccttc
     5281 gaggggtcct tcgcgtgcag gaactcgatc ggcgtgccct ggatcgtgtc tcccgcgtcg
     5341 aggaggagaa cgttcggcga ctccctgcgg atcgcctcga cgcgcgtcgc gacgcgggcg
     5401 agaccgtcgt ccgcgggctg ggcgcgcagg tagtcccagg gaaggaggtg gccgtgcagg
     5461 tccgtcgtct ggaggatcgt gacgacgacg cggtccgccg cggcggaaga agaggatcct
     5521 atgaaagtga agacgaggag ggagaggaga aagagaggag tggtgcgaag gcggcggtgc
     5581 ggacgtctca aagtccgcga atcttggcat atcctccggc cccatgccct cgaagaaatc
     5641 tccgcccgcg cccgcgcccg tgcccgagac gctcgcccgc ctcgagcacc tctacggcga
     5701 cgcgggctgc tcgctcgact tcgggagtcc gctccagctc ctcacggcga cgatcctctc
     5761 ggcgcagtgc acggacgcgc gcgtgaacat ggtgacgccc gaggtcttca agcgcttccc
     5821 cgacgccgcc gcgctcgcgg ccgcgccgcc gggcgccctc gaggacgtga tccgctccac
     5881 cggctttttc aacgcgaagg cgaagtccct ccgcgggatg gcgaaggcgc tcgtcgagaa
     5941 gcacggcggc gaggtcccgc gcacgatggc cgagctccac gccctgcccg gcgtcggccg
     6001 caagaccgcg aacgtcgtcc tcggcaacgt gtggaacacg ccggaaggcg tcgtcgtgga
     6061 cacgcacgtc ggccgcctcg cgaggcgcct cgggtggacg aagaacgccg accccgtgag
     6121 ggtcgaacag gacctgaacg agaggatccc gcaggagcgc tggacgtgga tcagccacgc
     6181 cctcatcctc cacggccgcc gcatctgcgc ctcgcggcgg ccgaagtgcg acctctgcac
     6241 cctcgccgac atctgcccga aggtgggggt cgacgaaaag aagaagaaac aagagacaga
     6301 gagaaaaaac aaaagaaaga cgaagatttc tttgaaggtg aagagggcag gggcgacagg
     6361 cgcggcgaag cgggctgcgg ctgcaaaggc ccctcacgcg ccttctaaga aaatcctctc
     6421 cttctcctcg tccccctctt ccgcgcggcc ttcggctgcg cgcaaaggac ctcgtcgatg
     6481 acgcctcccg gaatcggatc gatcgaagtc atctgcggct cgatgttctc gggcaagtcc
     6541 gaggagctca tccgccgcct cacgcgcgcg cagatcgcgc gccgccgagt ccaggcgttc
     6601 aagccggtcc tcgacaaccg ctacgccgag atggaggtcg tctcgcacgg cggcctgcgt
     6661 ctcgtggcgg tcgccgtgac cacgtccgcc gaggtcctcg cacgaaccga cgcgcgcacg
     6721 gaagtcgtcg ggatcgacga ggcccagttc ttcgacgccg cgatcgtcga ggtcgtctcg
     6781 cggctcgcgg acctcggcaa gcgcgtgatc atcgccgggc tcgatcagga ctacctcggc
     6841 cgcccgttcg agccgatgcc ggcgctgatg gccgccgccg aggaggtcac gaagacgcgc
     6901 gcgatctgcg tccgctgcgg ccagcccgcg agccgcacgc agcgcctcgt cgagtccacc
     6961 gagcgcgtcg tcgtcggcgc cgccggcctg tacgaagccc gctgccggcg ctgtttcgag
     7021 cccggcgtcg cgagctcgga gggcaagctg ccctttccgg acggctcttc aaccgattga
     7081 gttcgaagag gttgcggaga tcctcagagg atctgtgaat tctcctgtgg attccggtgt
     7141 gcaaaagcgg gcgctccccc gtccttcgcg gacttcacaa ttcctgcacg gattcctgtg
     7201 cagcggccaa accgctgaaa atgtttgggt tcaccgcgca cggcctctgt gaagccggcg
     7261 cgaagccgca tggatggccg gttccggcga attacacagg gcgaaatcgg cttcccatgc
     7321 gggtttgatc gcgctcacaa tcggaaaacc ctcggaaaaa tgcctgtttg acgcgatctt
     7381 gcggccttcg gcccctacca gcccgcgctt tcccggtgct cgccgaacac ggaacgcagc
     7441 gcctccacga cctcaccgag cgtcacgagg cccgagacgg cctcgacgat cggcgggagc
     7501 aggttgcggc cctcgcgcgc cgcgtcggcg acccccttgc gcaggcggtc cgcggccgcg
     7561 gcgtcgcgcc gctcccggag agccttgagc cgcgcgacct ggtctgtctc gacggcgggg
     7621 tcgatgacga gcgtcgggat cgggcgttcc tcgcccacgg tgaactcgtt cacgccgacg
     7681 acgatctgct ccttgtcctc gatcgcgcgc tgcgcgcggt aggccgcgtc ctggatctcg
     7741 cgctggtaga agccggcctc gatcgccttg agggcgccgc ccatcccgtc gatccgcgcg
     7801 ataagcgcgc cggcctcggc gacgatgccc gccgtgaggg cctcgatcgc ccacgagccg
     7861 ccgagcgggt ccgcgacgcg cgcgacgccc gtctcgtgcg cgatgacctg ctgcgtccgg
     7921 agcgcgagga gcgccgaatc ctccgtgggc agcgacaggg cctcgtcctt tccgttcgtg
     7981 tggagggact gcgtgccgcc gagcacggcc gcgagcgcct ggatcgcgac ccggacgacg
     8041 ttcacctcgg gctgctgcgc ggtcagcgtc gagcccgccg tctgcgtgtg gaagcgcagg
     8101 gcggccgcgc gcgggtccgt cacgccgaag cgctcgccga cgaggcgcgc ccagagggtg
     8161 cgagccgcgc ggaacttcgc g
//
LOCUS       DAOMFY010000171         8106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2762_length_8106_cov_8.740653, whole genome shotgun sequence.
ACCESSION   DAOMFY010000171 DAOMFY010000000
VERSION     DAOMFY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2762_length_8106_cov_8.740653"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLB01_13135"
     CDS             complement(<1..287)
                     /locus_tag="PLB01_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ78291.1"
                     /translation="MPAPQKDRLSQILNLTVFVAALGYFVDMFDLLLFPIVRQPSLTD
                     LGVPQGDPQVSATFLLLNAQMVGMLLGGILWGVLGDKKGRLSTLFGSIALYS"
     gene            348..1358
                     /locus_tag="PLB01_13140"
     CDS             348..1358
                     /locus_tag="PLB01_13140"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQQ78292.1"
                     /translation="MRDPEMPEASPATPGVEAPRGRRKWVTHGLSNRVVYGGLHYGAR
                     WLPLPVLHGISLVGNTLAVTLMRETLEGIRENFRLALAAPEPEARRLARRLFFEYGKA
                     VIDVWRLRSEAFVPKITSFDADAARLAGVRRNGRGFLLVTGHVGNWEMGAVTLRGHGL
                     TPAVVGQPELDPDVEEMRLSLRSRLGVESIDIGSSMATAFRVRAAIEAGRAVALLVDR
                     AYPEDQVVVSYFGRPTPFLRSPALLARFCKCEILPGFFMRSPDGSYFNVWGAPLAADE
                     SLSPEEDARRIMSRVAADLEGVVRAHPTQWFNFYRFWKDGSASAGAAGDTPERGGRVH
                     AP"
     gene            complement(1274..2581)
                     /locus_tag="PLB01_13145"
     CDS             complement(1274..2581)
                     /locus_tag="PLB01_13145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ78293.1"
                     /translation="MSFMRGLKLAGFARRHLGRMVRGRREVMTHLSAPDPGNLPMPTF
                     VQLRVTNLCNLRCKMCGQWGDTGIYRGGADGPASDGQRERDRIRELIGLNRQMTLADY
                     VRLLDEIAPSQPIISLFGGEPLLYPDILPLIREIKGRGLTCSIITNGWRLEEMARDLV
                     EAGVDSIMVSIDGTPEVHDRIRGQAGSFARAAAGVTAIARLRGELGSAVPMLLAILPI
                     TELNLDDVAPALEALQALPLDTVNVGLRWFVSKDTGAAYERVMSETFGVSGSSWKGFE
                     FDGADFAATKASQLTQLAKLLKGLRRRRFLDSTRGRPWVSFVPDVPAARVPDYFADPG
                     KTFGHDLCPVAWYFAQVEPDGEVCFCGDFPDYFIGNVRKTPFREIWSGERAGLFREKL
                     AREPLPICSRCCGSWVYGAWTRPPRSGVSPAAPAEALPSFQNR"
     gene            complement(2663..3253)
                     /locus_tag="PLB01_13150"
     CDS             complement(2663..3253)
                     /locus_tag="PLB01_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78294.1"
                     /translation="MISVERDSSGRTYQIWKQDQLNSCGVASAWMARGIVMQMSLNED
                     EWSLAQRIYRQAVDNALAPLGVPSSSGPMSLNPGSFANNQSSMASTIANFGFYAAQLA
                     HALRAQGLKVEHVGFNGQPRIVTASKISISKPAIALVCWNGGGGHFVVVGRATQSLIS
                     FLDPWDGRVNEQPNNGRYRARYNNQGYIGEILYLSI"
     gene            complement(3372..4847)
                     /locus_tag="PLB01_13155"
     CDS             complement(3372..4847)
                     /locus_tag="PLB01_13155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQQ78295.1"
                     /translation="MPDNVHDGTPLTYVDTAWLHMEDPTNLMMVTGIFIFDGPLDLAR
                     VRDTLQARMVDRFPRLRHRVKQDGSSAHWVEDPTFSLDAHVHKMALPAPGDLGTLRDV
                     VSDLMSTPLDFTKPLWQCHYIEGLGAGSAILLRFHHCIGDGVALVHVILSLTDAAADA
                     PRPRRTRLLAHETHVGERIVSLFDEARSAVERTVGATRKVTGAILEEGVETLLHPSHV
                     LDIAQTTTEAAGVLLKLILKPADSATLLKGHLGVAKRAAWSRPLRLDEVKAVGKSVDG
                     TVNDVLLTAVAGALRRYLLQRGDRADGVEINAMVPVNLRPLSEADQLGNRFGLIYLAL
                     PVGTRDRPERLRLVKTRMDEIKSSPQPFVTFQILNALGLAPAEVAGLAVNMFGTKATA
                     VMTNVIGPKQPLYFAGERIRTWLGWVPQSGHMGLGVSIFSYDGQVVLGVASDKGLVPD
                     PESIASAFEEEFEALVEWARGTGATGAATKRKKAGPVKNAT"
     gene            complement(4901..6106)
                     /locus_tag="PLB01_13160"
     CDS             complement(4901..6106)
                     /locus_tag="PLB01_13160"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HQQ78296.1"
                     /translation="MFGLMKARSCALDDDAKETRRLQYCGTCKTLGALYGQRSRLLLN
                     HDTVFLGEVLSALEEGARGETAPDPSIASRNCLALPARDAARTTPLAFAAAATVVLAE
                     AKLADHAADSGKLRWRLGRRFYSRTFEDASHWLREQGFPIDEVTEALAGQEAREARAV
                     AVPSGPSAEASLESLSEPTAFATGAFFAHGAQFAGRPEAEEALGALGRAFGSLVYRLD
                     AFEDYDRDARSGEFNAYRAVRGGVGPLSGEDSLAIRQRIWSQGVEICDLIRSLPIPPA
                     RAEVFAERLRTNLAARLGLSPKQAACTCVSALKSSKPAEDAAAEAAPARPLRESLALL
                     LNLPFLASAVSGLASPLYFSASGPGGLPGAAGEAVKHVKKGGGGKGGSDCGCCDACDC
                     GDCCCDCDC"
     gene            complement(6273..8075)
                     /locus_tag="PLB01_13165"
     CDS             complement(6273..8075)
                     /locus_tag="PLB01_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78297.1"
                     /translation="MPAADRRRPSRPLAIAAALGAAALAAAAVWTLKPAPAVPRHPLV
                     RTSYPMGPQLGLMIQRRLLAIAPDGRTLAMVVGSLGTGSHLEVRRLDELAPSRVKGSE
                     GIQSVFFSPDSRFIGFTTDTAIKKVAIGSNEGVTVADIGTNLSSGPNGIAWGDNGIIY
                     FGTLDGIRAVPATGGAAQLVVAGRKLSHPQVLPGSRFLLYARQPQTAFTPDGAVVLRS
                     LGDGTETELTTGTSPVCPAGDVLLVARADGVFAAPLSLAGRRLTKEPVLVVPGVALVG
                     SAAQYDLSKTGTLVYMPGSVLNESLCLPRRVQATGLAAPLSKTVHEYSDPRVSPDGRH
                     LALHLQDEGDDVWAFDVARDSLTRLTFEPGEDETPVWSPDGRWIAFSATRGEKRCVIR
                     RPADGSGAEEVLWTLSDHAHVTDWAPDGRSLVVDVYRVKSRSDIVRLELGEKPTPHPF
                     LETPFDESSGRVSPDGRWIAYRSNESGRDEVYLQPFPNGGGKVSISNGGGVQPVWARD
                     GKALYFRSDTDLMVARLGAAPAPAFLPAISVFKDRFVRPQGAAHTTYDVFPDGSFVFL
                     EPTSLPDGSAAPSRSVIAAFHWLENLEMRVEAKP"
BASE COUNT         1213 a         2840 c         2863 g         1190 t
ORIGIN      
        1 gagtagagcg cgatgctccc gaagagcgtc gagaggcggc ccttcttgtc gccgaggacg
       61 ccccagagga tcccgcccag gagcatcccc accatctggg cgttgagcag caggaacgtg
      121 gcggagacct gtgggtcccc ctgagggacg ccgaggtccg tcaggctcgg ctgccgcacg
      181 atggggaaga gcagcaggtc gaacatgtcg acgaagtacc cgagggccgc cacgaagacg
      241 gtcaggttca ggatctgcga caggcggtcc ttctgcggcg ccggcacggg agccattctg
      301 cctcgcggcg ctgcgaatcg cgcgtgcggc tcgttatagt ccgggcgatg cgcgacccgg
      361 agatgcccga agcgagcccg gcgacgcccg gcgtggaagc gccgcgcggc cgccggaaat
      421 gggtcacaca cggactgtcg aaccgcgtcg tctacggcgg gctccactac ggcgcccgct
      481 ggctgccgct gccggtgctg cacgggatct cgctcgtcgg aaacaccctc gccgtgacgc
      541 tcatgcgcga gacgctcgag ggcatccgcg agaacttccg cctcgcgctc gccgctccgg
      601 agccggaggc gcgccgcctg gcccgccgcc tgttcttcga gtacggcaag gccgtgatcg
      661 acgtctggcg gctgcggagc gaggccttcg ttccgaagat cacgtcgttc gacgcggacg
      721 ccgcccgcct ggccggggtc cgccggaacg gccggggatt tctcctggtg accgggcacg
      781 tcggaaactg ggagatgggc gccgtgacgc tgcgcggcca cggcctgacg ccggccgtcg
      841 tcggccagcc ggaactcgat ccggacgtcg aggaaatgcg cctctccctg aggtcccgcc
      901 tcggcgtcga gtcgatcgac atcggctcct ccatggccac cgcgttccgg gtgcgcgcgg
      961 cgatcgaggc ggggcgggcg gtggcgctgc tcgtcgaccg cgcctatccg gaggaccagg
     1021 tggtcgtctc ctacttcggc cgccccacgc cctttctccg ctcgccggcc cttctcgccc
     1081 gcttctgcaa gtgcgagatc ctgcccggct tcttcatgcg cagccccgac ggctcgtact
     1141 tcaacgtctg gggcgcaccg ctcgccgccg acgagtcgct ctcccccgag gaggatgcgc
     1201 gccgcatcat gagccgcgtg gccgcggacc tggagggcgt cgtgcgcgcc cacccgacgc
     1261 agtggttcaa cttctaccgg ttctggaagg acgggagcgc ttcggcgggc gcggccgggg
     1321 acacgccgga gcgaggcggg cgcgtccacg cgccgtagac ccagctcccg cagcaccggc
     1381 tgcagatggg gagcggctcg cgcgcgagct tctcgcgaaa gagcccggcg cgctccccgc
     1441 tccagatttc ccggaacggc gtcttccgca cgttgccgat gaagtagtcg ggaaagtctc
     1501 cgcagaagca gacctcgccg tccggctcga cctgcgcgaa gtaccaggcc acggggcaga
     1561 ggtcgtgccc gaacgtcttg cccgggtcgg cgaagtagtc gggcacgcgg gccgcgggca
     1621 cgtccggcac gaacgagacc cagggacggc cgcgggtgga gtcgaggaag cggcggcggc
     1681 ggaggccctt gaggagcttc gcgagctggg tcagctggct cgccttcgtg gccgcgaagt
     1741 ccgcgccgtc gaactcgaat cctttccagg aggagccgga gacgccgaag gtctcggaca
     1801 tcaccctctc gtacgccgcg ccggtgtcct tcgacacgaa ccagcgaagc ccgacgttga
     1861 ccgtgtcgag cggaagcgcc tgcagggcct cgagcgccgg cgcgacgtcg tccaggttga
     1921 gctcggtgat cggcaggatc gcgagcagca tcggcacggc ggacccgagc tcgccgcgca
     1981 agcgggcgat ggccgtgacg ccggcggcgg cgcgcgcgaa gctgccggcc tgtccccgga
     2041 tccggtcgtg gacctcgggc gtgccgtcga tggagaccat gatcgaatcc acgccggcct
     2101 caacgaggtc gcgggccatc tcctcgagtc gccagccgtt cgtgatgatc gagcacgtca
     2161 ggccgcgccc cttgatctcc cggatgagcg gcaggatgtc cggatacagc agcggctcgc
     2221 cgccgaacag gctgatgatg ggctgcgagg gcgcgatctc gtcgaggagg cggacgtagt
     2281 ccgcgagcgt catctgccgg ttcagaccga tcagctcgcg gatcctgtcc cgctcacgct
     2341 gtccgtcgga cgccggcccg tcggcgcctc cgcggtagat ccccgtgtct ccccactgtc
     2401 cgcacatctt gcagcggagg ttgcagaggt tcgtgacgcg gagctgcacg aacgtgggca
     2461 tcgggaggtt gccggggtcg ggcgcggaaa ggtgcgtcat cacctcgcgg cgcccgcgca
     2521 ccatgcggcc gaggtgccgg cgcgcgaatc ccgcgagctt caggccgcgc atgaagctca
     2581 tggcgccttc ccggcctcga gagtccccgt tgctcgctcg aacgcgcccc ggctctgcat
     2641 cgctctgtcc atgttccgcc ggtcagatcg agagatacag aatctcgccg atgtaaccct
     2701 ggttgttgta gcgggcccgg tagcggccat tgttgggctg ctcgttgacg cggccgtccc
     2761 agggatccag gaacgagatc aggctctggg tggcgcgccc gaccaccacg aagtggccgc
     2821 cgccgccgtt ccagcacacg agcgcgatgg ccggcttgct gatcgagatc ttcgaggccg
     2881 tgacgatgcg cggctggccg ttgaaaccga cgtgctcgac cttcagccct tgcgcccgga
     2941 gcgcatgcgc gagctgcgcg gcatagaacc cgaaattggc gatggtggac gccatgctgc
     3001 tctggttgtt cgcgaacgag cccgggttca gcgacatcgg gcccgacgac gacggcacgc
     3061 ccagcggtgc gagcgcgttg tcgaccgcct ggcggtagat cctctgcgcg aggctccact
     3121 cgtcctcgtt gaggctcatc tgcatgacga tgccccgcgc catccaggcg ctggcgacgc
     3181 cgcaggagtt caactgatcc tgtttccaga tctggtaggt ccgcccgctg ctgtcgcgtt
     3241 cgacgctgat catggccgcc tccaaagaag agacagggaa cagggcacac ggatcggacc
     3301 ttcccctcag gggaacgatc atgacacccg tagccccttc tgatccagct tctcgagcgc
     3361 agcgccggga ttcaggtcgc gttcttcacg ggcccggctt tcttcctctt cgtcgccgcc
     3421 ccggtggcgc ccgtcccgcg ggcccattcg acgagcgcct cgaactcctc ctcgaaggcg
     3481 ctcgcgatcg actccggatc gggaacgaga cccttgtcgc tggcgacgcc gaggacgacc
     3541 tgcccgtcat agctgaagat cgagacgccc aggcccatgt ggcccgactg cggcacccag
     3601 ccgagccacg tgcggatcct ctcgccggcg aagtagagcg gctgcttcgg cccgatcacg
     3661 ttcgtcatca cggccgtcgc cttcgttccg aacatgttca cggcgagccc ggcgacctcg
     3721 gcgggggcca gccccagcgc gttcaggatc tggaaggtca cgaagggctg gggcgagctc
     3781 ttgatctcgt ccatccgcgt cttgacgagc cgcagccgct cggggcggtc ccgggtgccg
     3841 acggggagcg cgagatagat gagcccgaac cggttcccca gctggtccgc ttcggacagg
     3901 ggacggaggt tgacgggcac catggcgttg atctcgacac cgtcggcccg atcgccgcgc
     3961 tggaggaggt agcgccggag cgccccggcg accgccgtga gaaggacgtc gttcaccgtt
     4021 ccgtcaacgg acttgccgac ggccttgacc tcgtccaggc ggaggggccg cgaccaggcc
     4081 gcgcgcttgg cgaccccgag gtgccccttg aggagcgtgg cggaatccgc cggcttcagg
     4141 atcagcttca ggaggactcc ggcggcctcc gtggtcgtct gcgcgatgtc caggacgtgc
     4201 gacgggtgga ggagcgtttc caccccttcc tccaggatcg ctcctgtcac ctttcgcgtg
     4261 gccccgaccg ttctctccac cgccgaccgc gcctcgtcga acagcgagac gattcgctcg
     4321 ccgacgtgcg tctcgtgggc gaggagcctg gttcgccggg gccgcggcgc gtcggccgcg
     4381 gcgtccgtga gcgagaggat cacgtggacg agcgccacgc cgtcgccgat gcagtggtgg
     4441 aagcgcagga ggatcgcgct cccggcgccg agtccctcga tgtagtggca ctgccagagc
     4501 ggcttcgtga aatcgagcgg cgtcgacatg aggtcgctca cgacgtcgcg gagcgtcccg
     4561 aggtcgccgg gagccggcag ggccatcttg tggacgtggg cgtccaggct gaaagtcggg
     4621 tcttcgaccc agtgggcgga ggacccgtcc tgcttgaccc tgtgccggag gcgcgggaac
     4681 cggtccacca tgcgcgcctg cagcgtgtcg cgcacgcggg cgaggtcgag cggcccgtcg
     4741 aagatgaaga tccccgtcac catcatgagg ttcgtggggt cttccatgtg aagccaggcg
     4801 gtgtcgacgt acgtcagcgg cgtgccgtcg tggacgttgt ccggcatgag gatgaagatg
     4861 gggccccgcc cgagccggcg caagacggag ggtcagagaa tcagcagtcg cagtcgcagc
     4921 agcagtcccc gcagtcgcac gcgtcgcagc acccgcagtc gctcccgccc ttgccgccgc
     4981 cgcccttctt cacgtgcttc acggcctcgc ccgccgcccc cggaagcccg cccgggcccg
     5041 atgccgagaa gtagagcggc gacgcgaggc ccgagaccgc cgaggcgagg aacggcagat
     5101 tgagaaggag tgcgagcgac tcgcgcaggg gacgcgcggg ggccgcctcc gccgccgcgt
     5161 cttcggccgg cttcgagctc ttcagcgcgc tcacgcacgt gcacgccgcc tgtttcggcg
     5221 agaggccgag ccgggccgcc aggttcgtcc ggagccgctc cgcgaagacc tccgcccgcg
     5281 ccggagggat cggaagactc cggatcaggt cgcagatctc gacgccctgc gaccagatcc
     5341 gctgccggat cgcgagggag tcctccccgg acagcggccc gacgccaccg cgcaccgcgc
     5401 ggtaagcgtt gaactcaccg gagcgcgcgt cccggtcgta gtcctcgaac gcgtcgaggc
     5461 gatagacgag ggagccgaac gcgcggccga gcgccccgag cgcctcttcg gcctccggcc
     5521 ggcccgcaaa ctgcgcgccg tgagcgaaga acgcgcccgt tgcgaaggcg gtcggctcgg
     5581 agagcgactc gagcgacgct tcggcggagg gtccgctcgg cacggcgacg gcgcgcgcct
     5641 cgcgcgcctc ctggccggcg agggcctccg tcacctcgtc gatcgggaac ccctgctcgc
     5701 ggagccagtg cgacgcatcc tcgaaggtcc gcgagtagaa gcgccgcccg agccgccagc
     5761 gcaatttccc ggaatcggcg gcgtgatcgg cgagcttcgc ctccgcgagc acgaccgtgg
     5821 ccgcggcggc aaaggcgaga ggcgtcgtcc gcgccgcgtc ccgcgcgggc agcgcaagac
     5881 agttcctcga ggcgatcgag gggtccggcg cggtctcgcc gcgcgcgccc tcctccaggg
     5941 ccgagaggac ctcgccgaga aacacggtgt cgtggttgag gagaagccgc gacctctgtc
     6001 cgtacagggc cccgagcgtc ttgcaggtcc cgcagtactg gaggcggcgc gtctccttcg
     6061 cgtcgtcgtc gagagcgcac gagcgggcct tcatgagacc gaacatcgcc acctccctgc
     6121 cggatcgtcg cgggcattct aagccgcgac tgagactcgg cgtcccctca agcccgagag
     6181 ggtgacgaat cgaggatcga gcggggcttc aggatcgccc ccgtacgcaa gccccgcgac
     6241 gcccgtcgcg gggtttcgac tcatcccccg ggttacggtt tcgcctcgac ccgcatctcc
     6301 aggttctcga gccagtggaa cgcggcgatc acgctccggc tcggggccgc cgacccgtcc
     6361 ggcaggctcg tcggttccag gaagacgaag gagccgtccg ggaagacgtc gtaggtcgtg
     6421 tgcgcggcgc cctgcggccg cacgaagcgg tccttgaaga cggaaatcgc cggcaggaac
     6481 gcgggcgccg gagccgcgcc gagccgcgca accatcagat cggtgtcgga gcggaagtag
     6541 agcgccttcc cgtcgcgcgc ccacacgggc tggacgcctc cgccgttcga gatcgacacc
     6601 ttgcccccgc cgttcgggaa gggctgcaga tagacctcgt cccggccgga ctcgttcgac
     6661 cggtacgcga tccaccggcc gtcgggagag acccggcccg aggactcgtc gaacggcgtt
     6721 tcgaggaacg ggtgcggcgt cggcttctct cccagctcca gacgcacgat gtcgctgcgg
     6781 gatttcaccc ggtagacgtc caccacgagg gatcggccgt cgggggccca gtcggtcacg
     6841 tgggcgtggt cggacagggt ccagagcact tcctcggcgc cgctgccgtc cgcgggtctc
     6901 cggatcacgc accgcttctc cccccgcgtt gcgctgaagg cgatccaccg gccgtcgggg
     6961 gaccagaccg gcgtctcgtc ctctcccggc tcgaacgtca accgcgtcag ggagtcccgg
     7021 gcgacgtcga atgcccagac gtcgtccccc tcgtcctgca ggtggagcgc gaggtggcgc
     7081 ccgtcgggcg agacgcgcgg gtccgagtac tcgtggaccg tcttcgaaag gggcgcggcg
     7141 agaccggtcg cctgcacgcg ccgcggaaga cagagcgact cgttgaggac gctgcccggc
     7201 atgtagacga gcgtccccgt cttcgagagg tcgtactgcg cggcgctgcc caccagcgcg
     7261 actcccggaa ccacgaggac gggctctttc gtgagccgcc ggccggcgag agacagtggc
     7321 gccgcgaaga cgccgtcggc gcgggcgacc agcagcacgt cgccggcggg gcagaccggg
     7381 ctcgttcccg tcgtgagctc ggtctccgtc ccgtccccca gcgaccggag gacgactgca
     7441 ccgtccggcg tgaacgcggt ctgcggctgg cgcgcgtaga ggagaaagcg gctgcccggg
     7501 agcacctgcg gatgcgagag cttccggccg gcgacgacca gctgcgcggc gcctccggtc
     7561 gccggcaccg cgcggatgcc gtcgagagtc ccgaaataga tgatgccgtt atctccccag
     7621 gcgatgccgt tcggccccga cgagaggttc gttccgatgt ccgccacggt cacgccctcg
     7681 ttcgagccga tcgcgacctt cttgatggcg gtgtccgtgg tgaagccgat gaagcggctg
     7741 tcgggcgaga agaagacgct ctggatcccc tcggatccct tgacccgcga cggcgccagc
     7801 tcgtcgagcc gccgcacctc caggtgcgat cccgtcccga gagatcccac gaccatcgcg
     7861 agcgtgcggc cgtcgggcgc gatggcgaga aggcggcgct ggatcatgag gccgagctgc
     7921 ggtcccatcg ggtagctcgt cctcacgagc gggtgccgtg gaaccgcggg cgcgggtttc
     7981 agcgtccaca ccgcggcggc cgcgagagcg gccgccccga gcgccgcggc gatcgcgaga
     8041 ggacgcgacg gacgccggcg atccgcggct ggcacatgat gcacttggcg accaccggcg
     8101 tcggct
//
LOCUS       DAOMFY010000172         8036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2792_length_8036_cov_6.318757, whole genome shotgun sequence.
ACCESSION   DAOMFY010000172 DAOMFY010000000
VERSION     DAOMFY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_8036_cov_6.318757"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PLB01_13170"
     CDS             <1..551
                     /locus_tag="PLB01_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78298.1"
                     /translation="ALFSALRIVGEVFDYLLVFASAVSFAGWTALALGAVRPLEERGR
                     GRAVGIGCVVVALLAAVSNARGLSQQQPIPVATRESVAAFTAALKAHLAAEGIRRPLV
                     YIAEGEPWVPAAGALLELERAGIDYAVEEDWTVMFGRNRRATGAEDGAVWFVEKETAP
                     LMRLLAAAGKTKLLGRPMPGAR"
     gene            complement(555..4442)
                     /locus_tag="PLB01_13175"
     CDS             complement(555..4442)
                     /locus_tag="PLB01_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78299.1"
                     /translation="MDGDPERRSAGPAPRRDARSRREGSDPLTAGAPLLAGFGGILLF
                     LAAGAGLVELLPALRQRPLAARLGWAYLLGVAAVPGAVYLLGIAFDVRIRRGVVLAPV
                     VLLALAGLAARALRGRTSATWASCPPRAGRLAARVAFGVAAAVAVGLLADSATNRESG
                     WDSEMTWSAAARWVRADRSVTPRALTDARVFVDHPQYPLLMPIAQIVVQETFATGDDP
                     RAIKPLYAAFFPALLLVFFDLARRHAGTAATALASLAFAVTPLLPFERSGGAAGAYSD
                     VPLGAFWGASLLLLFGRTRASEGVAAAILLGAAVLAKNEGLPLALVALVVGVLAALLD
                     RPAARAHRIRALALAAGAVVAAALALHAWKAKIPQRWTLDYVAELRAVSLAKEAAARL
                     PLLPAAIGRETASWEDWSGLWLAALAALFAGASGLRRRVVPPVVLALAGCLTLYVGAM
                     LVSSWGGAVQVHSTWNRLLLQLTVPLSLLVALALRAGLRARGAALAWVNGADIIPAMP
                     PEPTAPGRAFRREALVFFAFLALTVAMTWPWAKHLRDHASDAGDPYLNSWILWWDFHQ
                     TFHDPLHLFDGNIFFPYKLSLAFSEHNYGLALPLFPLFALGLRPLTAQSLLTLLGFAF
                     SGYGAFRLGRTLTGSTGAAWVAGIAFAFVPYRFGQLPHVNYLFAGWIPILVEALVLFV
                     RERSPRRAAWLGAAFFLNGLAVIHWLVLTLVPLAAIALVLAFRSGADRDRAGWKRGAL
                     AVGIAGVALLPFLLPYQRAAKLYGFTRGIEETRDFSAQPHDWLNADPRNHLWRGFSEF
                     PTPGERALFPGLLLLALPLAALLLTPRGDAAAEGAAAAPDRRVLFALDAVAVVAGFLA
                     LFAASPAGIHVRLAGKEVFRATEPWRALAVFLAALAVRWWLAYPAALPLVASRSLGAS
                     VRAIRRPDVIVVGALLALLGFLGSLGVRFPFHRVLFETISLFRSIRVPARWATIAYLG
                     LAVLAGAGVLALAEAWKRRRPGARTAPVFALACLAFLFEDRAAPLDLFRGEADPDEVT
                     RFLAQTPMRGGLVELPSGTPEHGNYRAVLRAADHGKPLVTAVSGFSSPIVRRIEEDER
                     KAPIPDDLLDFLESLPTSYVLVHESWLDPTMRTTHRAWIDRALVSGRLVFVKRFDGDA
                     RNDLFAVAKNEPGARPLEPLPWTPPAGLTAEGRPWRETAELTGNIDAPAEGAVVKGEL
                     RVTGWARVPGEDLEVTVLFDGEIRPASAWARVPRADVCSVVPTLVDCSRAGYDATYVL
                     GPADAGPHEIVVLFRSKDGRERHYPPRKFVWKP"
     gene            complement(4358..4810)
                     /locus_tag="PLB01_13180"
     CDS             complement(4358..4810)
                     /locus_tag="PLB01_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78300.1"
                     /translation="MKVRRSSSWRRALPAAGVLAVAAYLAYGVPAALVTSWTRAGASR
                     AAARDGAEAALARFRGPEYAASIARIRESLPEDAEYLILESPAQMMVRFDLAPRRAVF
                     GGNAKDLAANVTPALLPSLPKWTVIPSVDPPGPRLVETRVLAEKGAIP"
     gene            4904..6595
                     /locus_tag="PLB01_13185"
     CDS             4904..6595
                     /locus_tag="PLB01_13185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ78301.1"
                     /translation="MAAAFAVLALLRLATLAPDPWEWDEILFTEGVRDGLDVRVNHPH
                     PPGYPVFIRLGQGIRALGAEPFRATALAGAIGGYLSVLALYLLLRELGSLRAWALAGA
                     LFYALTPSVWLHGVRPLSDGPGAAAGLFAAVFLVRAIAGKGRRALFWAAAFTALAAGV
                     RPQVGLALLPAAAVAAFLVFRRERSVKPLLLAAVMGVLLSAAIWIPVFRSSGGFAVWK
                     DRFDGQMKYVTTFDSPKLRDLARLRFWKRWWLDPVGHGKLAGTFLLLGLAGAALERKK
                     AGMIFLVFVPVTLLTLGVLSAESAPRYVLAFWAAPCALAAFALERIERARFGRIAAPA
                     AGVLVLAAFAALGAPAVAEVASKASPSVAAMEAVRAAGVLPGDVYVTEPLRAHADEYF
                     RYTAHVVSETQPVAVPRSGVFVGADDRPFGVVPQRVFTFDHHARLLHISRGRYLRTEI
                     FEGRAGAGVFVPIVTRPGWQLEGRVELPEGTTLSVKSATPADVTLTGTAGGNGAEVEI
                     RTSSGVNRVEVAAGREETAFFQSAPDATQTLFSFSTRRGVLVLKDFRIVAQVPRP"
     gene            6592..7383
                     /locus_tag="PLB01_13190"
     CDS             6592..7383
                     /locus_tag="PLB01_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78302.1"
                     /translation="MSSRLGAFLPAAAVAALALATAPAQVRTAREWLVESPKGLARGE
                     DAARRELRGDAYASALDEVRALVPEGGSYAIANGGKGRPDQNWLRCDLAPRVPVALRP
                     ETCGAWFFERPPKVVPRVTVVVEKDGSIRIAQTRTLLTSLWSGLRGPQEDVPGWIDAP
                     AEDATVAGRVVVTGWCQEKGKGPCAAIRVWLDGVEVDAARIERFPRPDVQAAVPDIGD
                     CSRAGWRTAFEEGVLAPGRHCVAAALIVDGGRHRRIGPWAFTVAK"
     gene            7380..>8036
                     /locus_tag="PLB01_13195"
     CDS             7380..>8036
                     /locus_tag="PLB01_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78303.1"
                     /translation="MSGAPATASLAAVAIVAASGLALADLVPLLRARQVLGRLGWAWL
                     LGCAWLGGGAYAASHVLHLPLRRGVLLPILLVPVAAALARRLRATVAARSARIPLKPF
                     EGILLSLLFVFGIFVTASLVANGLATPVSDWDGKMTWVAQARFIRGARTVDAPALREA
                     SAWVIHPRYPVLMPVLQTAAQEIVDAPWDDRFVRPLYALFWPAFLLVLFDAASARAGR
                     L"
BASE COUNT         1101 a         2815 c         3083 g         1037 t
ORIGIN      
        1 cggcgctctt ttcggccctc cgcatcgtcg gcgaggtctt cgactacctg ctcgtgttcg
       61 cgtcggccgt ctcgttcgcg ggctggacgg cgctcgcgct cggcgcggtg cgcccgctcg
      121 aggagcgcgg gcggggccgg gccgtcggca tcgggtgcgt cgtcgtggcg ctcctcgccg
      181 cggtctccaa cgcgcgcggc ctctcgcagc agcagccgat tcccgtcgcg acgagggaga
      241 gcgtcgcggc gttcacggcg gcgctcaagg cccacctcgc ggccgagggc atccggaggc
      301 cgctcgtcta catcgcggag ggcgaaccct gggtgcccgc tgcgggcgcg cttctcgagc
      361 tcgagcgcgc cggaatcgac tacgcggtcg aagaggactg gacggtcatg ttcggccgca
      421 accgccgcgc gacgggggcc gaggacggcg ccgtgtggtt cgtcgagaaa gaaacggcgc
      481 cgctcatgcg gctcctcgcc gcagcgggga agacgaagct cctcgggcgc ccgatgcccg
      541 gcgcccgctg aacgtcacgg cttccagacg aacttccgcg gcgggtagtg gcgctcgcgg
      601 ccgtccttgg accggaagag cacgacgatc tcgtgaggtc cggcgtcggc aggcccgaga
      661 acgtaggtgg cgtcgtatcc ggcgcgggag cagtcgacga gcgtggggac gacgctgcag
      721 acgtccgcgc ggggaacccg ggcccaggcg gaggcgggcc ggatctctcc gtcgaagagg
      781 acggtgacct cgaggtcctc gcccgggacg cgggcccagc ccgtgacgcg gagctcgccc
      841 tttacgaccg cgccctcggc gggggcgtcg atgttgcccg tgagctcggc cgtttcccgc
      901 cacggacggc cctcggccgt caggcccgcg ggcggcgtcc aggggagcgg ctccagcggc
      961 cgggcgcccg gctcgttctt cgcgaccgcg aagaggtcgt tgcgggcgtc gccgtcgaag
     1021 cgcttgacga aaacgaggcg gcccgagacg agcgcgcggt cgatccacgc ccggtgcgtc
     1081 gtgcgcatcg tggggtcgag ccacgactcg tgcacgagga cgtacgacgt cgggaggctc
     1141 tcgagaaagt cgaggagatc gtccgggatc ggcgccttgc gttcgtcttc ctcgatcctg
     1201 cggacgatgg gcgacgagaa gcccgagacg gccgtgacga gcggcttccc gtggtcggct
     1261 gcccgcagca cggcccggta gttcccgtgc tccggcgtgc cggagggcag ctcgaccagg
     1321 ccgcccctca tcggcgtctg cgcgaggaat cgggtgacct catccggatc cgcctcgccg
     1381 cggaaaaggt caagcggcgc cgcgcggtcc tcgaagagaa acgcgaggca cgcgagcgcg
     1441 aagacggggg cggtgcgcgc gccggggcgg cggcgcttcc acgcctcggc gagcgcgagg
     1501 acgcccgccc cggcgaggac ggcgaggccg aggtacgcga tcgtcgccca gcgggccggg
     1561 acccggatgc tgcggaagag cgaaatcgtc tcgaagagga cgcggtggaa cgggaaccgc
     1621 acgccgagcg agccgaggaa tccgaggagg gcgaggagcg cgccgacgac gatgacgtcc
     1681 gggcgccgga tcgcgcggac gctcgccccc aggctgcgcg acgccacgag cgggagcgcc
     1741 gcgggatacg cgagccacca gcgcacggcg agcgccgcga ggaagacggc gagggcgcgc
     1801 cagggctcgg tcgcgcggaa gacctctttc ccggcgaggc ggacgtggat gccggcgggg
     1861 ctcgccgcga agagcgcgag gaagcccgcg acgacggcca cggcgtcgag agcgaagaga
     1921 acgcgccgat ccggggcggc cgccgcgcct tcggcggccg cgtctccccg gggcgtcagg
     1981 agcagtgcgg cgagggggag cgcgagaagc aggagccccg ggaagagcgc gcgctcgccg
     2041 ggcgtcggga actccgagaa tcccctccag aggtggttgc gcggatcggc gttcagccag
     2101 tcgtgcggct gcgcggagaa gtcgcgcgtc tcctcgatgc ctcgcgtgaa gccgtagagc
     2161 ttcgcggcgc gctggtacgg gaggagaaac ggcaggagcg cgacgcccgc gatcccgacg
     2221 gccagcgccc cccttttcca gcccgcgcgg tcccggtccg cgccggatcg gaacgcgagg
     2281 acgagcgcga tcgccgcgag cgggacgagc gtgaggacga gccagtggat caccgcgagg
     2341 ccgttcagga agaacgcggc gccgagccag gccgcgcgcc gtgggctgcg ctcgcgcacg
     2401 aagaggacga gcgcctcgac gaggatcggg atccagcccg cgaagaggta gttcacgtgt
     2461 ggcagctgcc cgaagcggta gggcacgaac gcgaacgcga tgcccgccac ccacgcggcg
     2521 cccgtggacc cggtgagggt gcgtccgagc cggaacgcgc cgtagcccga gaacgcgaag
     2581 ccgaggagcg tgagaaggct ctgcgcggtg agcggccgga ggccgagggc gaagagcggg
     2641 aagagcggca gcgcgaggcc gtagttgtgc tcgctgaacg cgaggctcag cttgtagggg
     2701 aagaagatgt tcccgtcgaa gaggtgtaac gggtcatgga acgtctggtg gaagtcccac
     2761 cagaggatcc acgaattcag gtacgggtcc ccggcgtcgg acgcgtggtc gcggaggtgc
     2821 ttcgcccacg gccacgtcat cgcgaccgtg agcgcgagga acgcgaagaa cacgagggcc
     2881 tcgcgccgga aagcgcggcc gggcgcggtg ggttccgggg gcatcgcggg gatgatatcg
     2941 gcgccgttca cccaggcaag cgccgcgccg cgcgcccgaa ggcctgcgcg cagcgcgagc
     3001 gcgacgagga gactcagcgg gaccgtgagc tggaggagca gccgattcca tgtcgaatgg
     3061 acctgcacgg ctcctcccca cgacgacacg agcatcgcgc ccacgtagag agtgagacac
     3121 ccggcgagcg ccaggacgac gggcgggacg acgcggcgcc tcagtccgga cgctcccgcg
     3181 aagagggcgg caagagcggc cagccagagg ccggaccagt cctcccagga cgccgtttcc
     3241 ctgccgatcg ccgccggaag gagcgggagc cgcgcggcgg cctccttcgc gagcgaaacc
     3301 gcacggagct ccgccacgta gtccagggtc cagcgctgcg ggatcttcgc cttccaggcg
     3361 tggagcgcga gcgccgccgc aacgacggcg cccgccgcga gcgcgagggc gcggatccgg
     3421 tgcgcgcggg cggccggccg gtcgagcagg gctgcgagga cacccacgac gagcgcgacg
     3481 agcgccagcg gcaggccctc gttcttcgcg aggacggcgg cgccgaggag gatcgcggcg
     3541 gcgactcctt cggacgcgcg cgttctcccg aagagaagga ggaggctcgc gccccagaac
     3601 gcgccgaggg gaacgtcgga atacgcgccc gcggccccgc cggaacgctc gaacggaaga
     3661 agcggcgtca ccgcaaaggc gagggaggcg agcgccgtcg ccgcggtgcc cgcgtgccgc
     3721 cgcgcgaggt cgaagaacac gaggagaagg gcggggaaga acgccgcata gaggggcttg
     3781 atcgcgcggg ggtcgtcgcc cgtggcgaac gtctcctgga cgacgatctg cgcgatgggc
     3841 atgaggagcg ggtactgcgg gtggtccacg aacacccgcg cgtccgtgag cgcgcgcggt
     3901 gtcacgctcc ggtcggcgcg cacccagcgc gcggcagcgc tccacgtcat ctcggagtcc
     3961 caccccgact cgcggttcgt cgcggaatcc gcgaggaggc ccacggccac agccgccgcg
     4021 accccgaagg ccacgcgcgc ggcgagccgg ccggcccggg gcgggcagga tgcccacgtg
     4081 gcggaagtcc gcccccggag cgctcgggcg gcgaggcccg caagcgcgag gaggacgacc
     4141 ggcgcgagga cgacgccccg gcggatccgg acgtcgaacg cgatcccgag gagatagacg
     4201 gcgcccggca ctgcggcgac gccgaggaga tacgcccagc cgaggcgcgc ggcgagcggc
     4261 cgctggcgca gcgcgggcag gagctcgacg aggccggccc ccgccgcgag gaagaggagg
     4321 atgccgccga atccggcgag aaggggcgcg ccggcggtca ggggatcgct cccttctcgg
     4381 cgagaacgcg cgtctcgacg aggcgcgggc ccggcggatc gacgctcggg atcaccgtcc
     4441 acttcgggag cgagggcagg agagcgggtg tgacgttcgc ggcgaggtcc ttcgcgttgc
     4501 ctccgaagac ggcccggcgc ggtgcaaggt cgaagcgaac catcatctgg gccggggact
     4561 cgaggatcag gtactcggcg tcttccggca gggactcgcg tatccgcgcg atcgacgcgg
     4621 cgtactccgg gccgcgaaac cgcgcgagcg ctgcctcggc gccgtcccgc gcggccgccc
     4681 gggacgctcc ggcgcgggtc cacgacgtga cgagggccgc gggaacgccg tacgcgaggt
     4741 acgccgcgac ggcgaggacg cccgcggcgg gcagggcccg gcgccaggag gaggagcgac
     4801 ggactttcac gagccgcagg atgatatcgg cgtccggccg aatgcgtatg ctctcggggt
     4861 cctcatgtcc ttgagccgag cgtcccgccg ggagacagcc ctcgtggccg ccgcgttcgc
     4921 cgtcctcgcg ctcctgcgcc tcgcgacgct cgcgcccgat ccgtgggaat gggacgagat
     4981 cctcttcacg gagggcgtgc gcgacggcct cgacgtccgc gtcaaccatc cgcacccgcc
     5041 gggttacccg gtcttcatcc ggctcgggca ggggatccgc gcgctcgggg ccgagccgtt
     5101 ccgcgccacc gctctcgcgg gcgcgatcgg cgggtacctc tcggtcctcg cgctctacct
     5161 gctcctccgc gagctcggct cgctccgcgc ctgggcgctc gccggagcgc tcttctacgc
     5221 gctgacgccg tcggtgtggc tgcacggcgt ccggccgctg tccgacgggc cgggagcggc
     5281 cgccggcctc tttgccgcgg tgttcctcgt ccgcgcgatc gcggggaagg gccgccgcgc
     5341 cctcttctgg gccgcggcgt tcacggcgct cgcggcgggg gtgcgtccgc aggtcggcct
     5401 cgcgctcctg cccgcggcgg ccgtcgcagc ctttctcgtc ttccgtcgcg agcggagcgt
     5461 gaagccgctt ctccttgcag ccgtcatggg cgttctcctc tccgccgcga tctggatacc
     5521 ggtcttccgg agctccggcg ggttcgcggt ctggaaggac cggttcgacg ggcagatgaa
     5581 gtacgtcacg acgttcgaca gtccgaagct gcgcgacctc gcccggctcc ggttctggaa
     5641 gcgctggtgg ctcgacccgg tcgggcacgg gaagctcgcc gggacgttcc tgctgctcgg
     5701 cctcgcgggc gctgcgctcg agaggaagaa ggcggggatg atcttcctcg tcttcgtccc
     5761 ggtcacgctt ctcacgctcg gcgtcctgtc agccgagtcg gcgccgcggt acgtcctcgc
     5821 gttctgggcc gcaccgtgcg cgctcgccgc gttcgcgttg gagaggattg aacgggcacg
     5881 attcggccgg atcgccgcgc ccgcggccgg cgtcctcgtc ctcgccgcgt tcgcggctct
     5941 cggggcgccc gcggtcgccg aggtcgcgtc gaaagcgagc ccgtcggtcg ccgcgatgga
     6001 ggccgtccgc gccgccggcg tcctgcccgg cgacgtctac gtgaccgaac ctctgcgcgc
     6061 tcacgcggac gagtacttcc ggtacaccgc ccacgtcgtg agcgagaccc agcccgtcgc
     6121 cgtcccccgt agcggtgtgt tcgtcggcgc cgacgaccgg ccgttcggcg tcgtcccgca
     6181 gcgcgtcttc acgttcgacc accacgcgcg tctcctccac atctcgcgcg gccggtacct
     6241 gcgcacggag atcttcgagg gccgggcggg tgccggagtg ttcgtcccca tcgtcacgcg
     6301 gcccggctgg cagcttgagg ggcgggtcga gctcccggag gggacgacgc tctcggtcaa
     6361 gagcgcgacg cccgcggacg ttacgctcac gggaaccgcc gggggaaacg gagccgaagt
     6421 ggagatccgg acgtcgtcgg gggtgaaccg tgtggaggtt gccgccggac gcgaggagac
     6481 ggcgttcttc cagtccgctc cggacgccac gcagacgctc ttctcgttct cgacacggcg
     6541 tggcgtcctg gtcctcaagg acttccggat cgtcgcgcag gtcccgcgtc catgagcagc
     6601 cgcctcggcg cgttccttcc ggccgccgcc gtcgctgccc tcgcgctggc gacggcgccc
     6661 gcgcaggtgc ggacggcccg ggagtggctc gtggagagcc cgaaggggct cgcgcgcggt
     6721 gaggacgccg cgcgccggga gctgcgcggg gacgcctacg cgagcgcgct cgacgaggtg
     6781 cgggccctcg ttcccgaggg cgggtcgtac gcgatcgcga acggcggcaa gggccgcccc
     6841 gaccagaact ggctgcgctg cgacctcgcc ccgcgcgtgc cggtggccct ccggccggag
     6901 acgtgcgggg cgtggttctt cgagcgtcca ccgaaggtcg ttccgcgcgt cacggtcgtc
     6961 gtcgagaagg atgggagcat ccggatcgcc cagacgcgca cgctcctcac gtcgctgtgg
     7021 tccggcctcc ggggtcccca ggaggacgtc ccgggctgga tcgacgcgcc cgccgaagac
     7081 gcgacggtcg cggggcgcgt cgtcgtgacg ggctggtgcc aggagaaggg gaaaggcccc
     7141 tgcgcggcca tccgtgtctg gctggacggc gtcgaggtgg acgccgcgcg catcgagcgc
     7201 tttccgcgcc cggacgtgca ggcggccgtc ccggacatcg gcgactgcag ccgcgcgggc
     7261 tggcgcacgg cgttcgagga gggtgttctc gctccgggcc ggcactgcgt ggcggcggcg
     7321 ctcatcgttg acggcggacg gcaccgccgc atcgggccgt gggcgttcac ggtggcaaag
     7381 tgagcggtgc gcccgctacg gccagcctcg ccgccgtggc gatcgtcgcg gcaagcggcc
     7441 tcgcgctggc cgatctcgtc ccgctcctgc gcgcgcgcca ggttctcggg aggctcggct
     7501 gggcgtggct cctcggctgc gcgtggctgg gcggaggagc gtacgccgcg tcacacgtcc
     7561 tgcaccttcc gctccgccgc ggcgtgctgc tcccgatcct cctcgtgccc gtggccgcgg
     7621 cgctcgcgcg gcgcctgcgc gcaacggtgg cggcgcggtc cgcccggatc cctctcaagc
     7681 cattcgaagg aattcttctt tctcttctct tcgttttcgg catcttcgtc acggcaagcc
     7741 tcgtcgcgaa cggtctcgcg acgcccgtct ccgactggga cggcaagatg acgtgggtcg
     7801 cgcaggcgcg cttcatcagg ggcgcgcgga cggtggacgc gcccgccctg cgcgaggcct
     7861 cggcgtgggt catccatccg cgctacccgg tcctgatgcc ggtcctgcag acggccgcgc
     7921 aggaaatcgt cgacgcgcca tgggacgacc gcttcgtgcg tccgctctac gcgctgttct
     7981 ggccggcgtt tctcctcgtc ctcttcgacg cggcttcggc gcgcgccggc cgcctg
//
LOCUS       DAOMFY010000173         8030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2796_length_8030_cov_8.153730, whole genome shotgun sequence.
ACCESSION   DAOMFY010000173 DAOMFY010000000
VERSION     DAOMFY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2796_length_8030_cov_8.153730"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLB01_13200"
     CDS             complement(<1..668)
                     /locus_tag="PLB01_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78304.1"
                     /translation="MTPLPREEALRKAVHVGMAGFALLLRTLTWRQAAAAAAAALAIN
                     VFLVPRAAPNVLRVEERPKRFSVGIALYPLVVLLLVVTFQRNLPIAAAGWGYLAFGDG
                     FASIVGMTVGGPRLPWNPKKSLSGFLGYVVFGFLGAAALFGFVAAHVPSGWELGCLFA
                     AALAGAAVESLPSELDDNVLPPLVAAAALALLLPCRAGLTGALEPAGLRAFVVALGVN
                     AAVAG"
     gene            complement(665..1522)
                     /gene="rfbD"
                     /locus_tag="PLB01_13205"
     CDS             complement(665..1522)
                     /gene="rfbD"
                     /locus_tag="PLB01_13205"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HQQ78305.1"
                     /translation="MQTVILGAGGMLGRALGREFPAAERFTRAALDITDVPAVHAAVQ
                     PGVRLVLNAAADTRVDAAETDPRHNRVNHVAVGLLAARCSEIGATLVHVSTDYVFSGR
                     SIRPYREDDPVDPVNAYGRGKLAGERRAFEEGDEVLVVRTSWVFGTGGSNFVDTILKR
                     VEAGSRELSIVDDQVGRPTYAGDLAAAIRSLIDRRVTGIVHFANGGDATWHDLAREAL
                     RLSGREDVLLRAVKTEEFPRPARRPKHSVLDTSLYERLTKVTPRPWPQALAAYLEERR
                     AVVSPAPFP"
     gene            complement(1562..2890)
                     /locus_tag="PLB01_13210"
     CDS             complement(1562..2890)
                     /locus_tag="PLB01_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78306.1"
                     /translation="MSLSSRHARTVRTLAGAALAAMGVLSPAVRAAGPAPASASCAAP
                     IVIDPASLPYAAAGIDTRSAPLDPTVPVFPCYGKLFLPAGGQVQPSKVVWFSFKPQAT
                     DTYRIDTLGSSPADYDTILGVYTGECGTLAPVSGVCGRNGFFPDDAPGTLRSSVTLNL
                     SAGTTYSIAVGAIGAPDPYTGLVEPSAGGTLNLNVARVAVAYAYTYLIPNFVRSGGFT
                     SDLYVTNLENADAQFLVQYLAHGNDGDQTIPARQAVAPAQLVAAGGSRFYQDVVGLYG
                     HADDWGALLIQSTRRLLVGSRAWAPAAGGGTIGPYTAGVDVSPGLAAPEALATGETGR
                     FTGVREDAAARTNLVFVNTAPVPCVLQAEVRDGAGAALGAARSLSVPPLTAMQKSRLK
                     DTFAIGADVRNASVVVRNATAGCSVIGVAYVFDVNASGAATNSYAVPLRK"
     gene            complement(2959..5187)
                     /gene="lptD"
                     /locus_tag="PLB01_13215"
     CDS             complement(2959..5187)
                     /gene="lptD"
                     /locus_tag="PLB01_13215"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HQQ78307.1"
                     /translation="MRRIGLVLLGFLAAGRAFSQSSFDTDPFASRFREKGGFEVKFKL
                     PEKGGSIRIAIDAGEEGGPQGRQTVVDEDLFTADAPPGKFVTVEYQDIRLTARRVTGS
                     LKRRTVVAEGDVTLVQGLSRMYGVRLDLDLNDKVGVLLDGRVDLEGGFHLKGATIAKV
                     GPKSFTLTEGTVTACDGDDPAWKFTVKSGRVTLEDYARLKNATFTLGGVPLLWTPYLL
                     WPALRDRASGFLVPGLGYNSTRGGYFGLSYYWAIDRSWDATFSGDVYTEKYYGFGAEV
                     RGQPSAGTRFEGTYYTIQDPTVDAWRWQTAGRLVSDDIAPKVRGVATWLNFSDPTFFQ
                     DFNRNFALGSTRTIGSEGFLTWSPDPWALNLRLSRQEAIGFSDGNSVVSERLPTLEAR
                     LRPTPLFGQSVFLDFLAQGGVLHTDRGPNQPAGTYDRFDLFPQISVPLSIAPWLSLDS
                     SAGGRVTSYGMSLSPDGTALLSERYTRQALVTNVALTGPSFSRLFDWKAGGFAKWKHV
                     IEPRAEWAYLVGRDDFSSTPLFDEIDTLIPTNALRYSLFQHLLAKGDTGGSREVASLE
                     IARYYYFRLPGEGTAYGPSPTAQRSSPTDFILRVNAAQGLNFDSRVTWDTHFGQVTSA
                     SVTAILTGGERSLVLSLFDSHPVIIPAEADPGASAQLRVAAGLPLLPKRLRFDIEANY
                     DLTKGRMLESRSLLTFSASCFRVLVEYRDLRTGETPSRDYRVALTLRNVGSFLDFTGS
                     LP"
     gene            complement(5217..5879)
                     /locus_tag="PLB01_13220"
     CDS             complement(5217..5879)
                     /locus_tag="PLB01_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HQQ78308.1"
                     /translation="MAKLDRDGIIRRAAQELRDGMVVNLGIGMPTLVSNFIPAGMEVI
                     LQSENGILGMGPYPTEAEVDPDLINAGKETVTVIPGASFFSSADSFAMIRGGHVDLCI
                     LGAMQVDAEGSIANWMIPGRLVKGMGGAMDLVAATRRIVVTMEHVAKNGEKKILNRCT
                     LPLTGRGCVHRIVTDLAVLDVKKGGGLVLVERAPGVSVEEIRAATEPPLDVSGDVPEM
                     KV"
     gene            complement(5879..6577)
                     /locus_tag="PLB01_13225"
     CDS             complement(5879..6577)
                     /locus_tag="PLB01_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HQQ78309.1"
                     /translation="MRGASPAEAAAKAVLDVPSGATLVVGGFGLCGIPENLILALAAS
                     DVSGLTCVSNNCGVDDWGLGLLLGKKKIRKMLSSYVGENAEFERQYLAGELELEFCPQ
                     GTLAERMRAGGAGIAGFYTPTGVGTIVAEGKETKVFDGKEYLLERGIVGDFALVAAWK
                     GDRLGNLVYRKTARNFNPMAATAGKITIAEVEQLVEPGEIDPDEVDTPGIFVQRVVVA
                     PRVKRIERRTVRKG"
     gene            complement(6608..7789)
                     /locus_tag="PLB01_13230"
     CDS             complement(6608..7789)
                     /locus_tag="PLB01_13230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQQ78310.1"
                     /translation="MSQDVFILSAARTPIGSFLGSLSTLPAHALGAVAIKAAVERAEV
                     AAARVEHVWMGNVLQAGQGQAPARQALLAAGLAKGTGAVTVHKVCGSGMRAVMDAANA
                     LRCGEFELAVAGGMESMSNAPYLLPKGRLGLRMGHGQLLDSMISDGLWDPYKNIHMGV
                     CAEMCAEKYAFTREAQDAFALESYQRARRANESGDFNAEFAPVTIEGKKGATVVDRDE
                     EPFATPLEKLEKMGSLKPAFQKEGTVTAANASKINDGASALVLAGEKAAASAKPIARI
                     LSQASFAQEPEWFTTAPVAAAKKAAERAGLPLASIDLFEVNEAFAVVAMAFVKDLDLD
                     PNKVNVNGGAVALGHPIGASGARILATLVHALKARKRKYGLASICIGGGEATAVVVEA
                     L"
BASE COUNT         1226 a         2765 c         2896 g         1143 t
ORIGIN      
        1 cccgcgacgg ccgcgttgac cccgagggcg accacgaacg cgcggagacc cgcgggctcg
       61 agcgcgccgg tcaggcccgc gcggcagggg aggaggagcg cgagggccgc ggccgcgacg
      121 agcggcggca ggacgttgtc gtccagctcc gacggcaggc tctcgacggc cgcgcccgcg
      181 agagccgccg cgaagagaca gccgagttcc cagcccgacg ggacgtgcgc ggcgacgaag
      241 ccgaacaacg ccgccgcgcc gaggaagccg aagacgacat agccgaggaa gccggagaga
      301 gacttcttcg ggttccaggg gaggcgaggc ccccccaccg tcatcccgac gatcgacgcg
      361 aagccgtcgc cgaaggcgag gtatccccag ccggcggcgg cgatgggaag atttctttga
      421 aaggtgacta cgaggaggag caccacgagc ggatacagcg cgatgcccac ggaaaagcgc
      481 ttcggtcgct cttccactct aagaacattc ggcgcggccc tcggcaccaa gaaaacattg
      541 atggcgaggg cagcggcggc cgcggccgcc gcctgccgcc acgtcagcgt cctgaggagg
      601 agcgcgaatc ccgccatgcc gacgtggacc gccttgcgga gtgcttcctc gcgcggcagc
      661 ggcgtcatgg gaacggggcc ggcgacacca cggcacggcg ctcctcgagg tacgcggcga
      721 gcgcctgggg ccagggccgg ggcgtgactt tcgtgaggcg ctcgtagagc gacgtgtcga
      781 ggaccgagtg cttcggccgg cgtgccggac gcgggaattc ctcggtcttg acggcccgga
      841 gcaggacgtc ctcgcgcccc gagaggcgga gcgcctcgcg ggcgaggtcg tgccacgtcg
      901 cgtcgccgcc gttcgcgaag tggacgatgc ccgtcacgcg ccggtcgatc agggaacgga
      961 tcgcggcggc gaggtccccg gcatacgtgg gccggccgac ctggtcgtcg acgatcgaga
     1021 gctcgcgcga gccggcctcc acccgcttca ggatcgtgtc cacgaagttc gagcctccgg
     1081 ttccgaagac ccacgacgtg cgcacgacga gaacctcgtc tccctcctcg aaggcccggc
     1141 gctcgccggc gagcttgccg cgcccgtagg cgttcaccgg gtccacggga tcgtcctcgc
     1201 ggtacggccg gatcgaccgg ccgctgaaga cgtagtccgt cgagacgtgg acgagcgtcg
     1261 cgccgatctc gctgcagcgg gcggcgagga ggccgacggc gacgtggttc acgcggttgt
     1321 ggcgcgggtc cgtttcggcc gcgtccacgc gcgtgtcggc ggccgcgttc aggacgaggc
     1381 gcacccccgg ctgcacggcg gcatgcaccg ccgggacatc ggtgatgtcg agggctgcgc
     1441 gcgtgaagcg ttcggccgcc gggaactcgc gcccgagggc gcggcccagc attccgccgg
     1501 ctccgaggat caccgtctgc acggaagagg cagtgtaccg gatgagaccc gcgccgcgcg
     1561 tctacttgcg caggggaacg gcgtaggaat tggtcgcggc gccgctcgcg ttcacgtcga
     1621 agacgtacgc cacgccgatg accgagcagc ccgccgtcgc gttgcggacg acgacggacg
     1681 cgttgcgcac gtcggcgccg atcgcaaacg tgtctttcag gcggctcttc tgcatcgccg
     1741 tcagcggcgg caccgagagc gagcgggccg ccccgagcgc ggccccggcg ccgtcgcgca
     1801 cctccgcctg gaggacgcac ggcacgggcg ccgtattcac gaagacgagg ttcgtgcggg
     1861 ccgccgcgtc ctcgcggact ccggtgaacc ggccggtctc cccggtcgcg agcgcttcgg
     1921 gagcggcgag gcccggcgag acgtccacgc cggccgtgta cgggccgatc gtgccgccgc
     1981 ccgccgcggg cgcccaggcg cgcgagccga cgagaagccg gcgcgtggac tggatgagga
     2041 gcgctcccca gtcgtccgcg tggccgtaga gcccgacgac gtcctggtag aagcggctgc
     2101 cgcccgccgc gacgagctgg gccggagcga ccgcctgccg cgcgggaatc gtctggtcgc
     2161 cgtcgttgcc gtgcgcgagg tactgcacga ggaactgggc gtcggcgttc tcgaggttcg
     2221 tcacgtagag gtcgctcgtg aatccgcccg agcgcacgaa gttcggaatg agatacgtgt
     2281 aggcgtacgc gaccgcgaca cgggcgacgt tgagattcag cgttccgccc gcggagggct
     2341 cgaccagtcc cgtgtagggg tccggagcgc cgatcgcgcc gaccgcgatc gagtacgtcg
     2401 ttcccgccga caggttgagc gtgaccgagg accggagcgt tccgggagca tcgtccggga
     2461 agaagccgtt ccggccgcac acgcccgaga cgggcgcgag cgtcccgcac tcccccgtgt
     2521 agacgccgag gatcgtgtcg tagtcggcgg gagacgagcc gagcgtgtcg atcctatagg
     2581 tgtccgtcgc ctgaggcttg aacgagaacc agaccacctt cgacggctgc acctgcccgc
     2641 ccgcgggaag aaagagcttc ccgtagcacg ggaacaccgg gaccgtcgga tcgagcggcg
     2701 ccgagcgcgt gtcgatcccc gcggcggcat aagggaggga ggccgggtcg atcacgatag
     2761 gggccgcaca ggacgccgat gcgggagccg gccctgcggc ccgaacggcc ggcgaaagga
     2821 ctcccatcgc ggccagggcc gctcctgcga gcgtccggac ggtgcgtgcg tgacggctgg
     2881 aaaggctcat gtacggtttt ttggatgccc ggcgggcacg gtccagtggg aagtgtctcg
     2941 cgaaccgcct atccctcgtc agggcaggct gcccgtgaag tccaggaagc tgcccacgtt
     3001 ccggagggtg agggcgaccc ggtagtcccg cgagggggtc tcccccgtgc gcagatcgcg
     3061 gtattcgacg aggacccgga aacacgacgc cgagaaggtc agcagcgagc gggactcgag
     3121 catccggccc ttcgtgaggt cgtagttcgc ctcgatgtcg aaccggagcc tcttcgggag
     3181 gagaggcagg cccgccgcga cccggagctg cgcggacgcg ccggggtcgg cctcggcggg
     3241 aatgatgacg gggtggctgt cgaagagcga gagcacgaga gaccgctcgc cgcccgtcag
     3301 gatggccgtc acgctcgcgg acgtcacctg cccgaagtgc gtgtcccacg tgacgcggct
     3361 gtcgaagttc agcccctgcg ccgcgttcac gcgcaggatg aagtccgtcg gcgacgagcg
     3421 ctgggcggtg gggctcgggc cgtaggccgt accttcgccg ggcagcctga agtagtagta
     3481 gcgcgcgatc tcgagcgacg cgacctcgcg cgacccccct gtgtcgccct tggcgaggag
     3541 gtgctggaag agggagtacc ggagcgcgtt cgtgggaatc aacgtgtcga tctcgtcgaa
     3601 gagcggcgtc gagctgaagt cgtcgcgccc gacgaggtac gcccactccg cgcgcggctc
     3661 gatcacgtgc ttccacttcg cgaacccgcc ggccttccag tcgaacaggc gcgagaagga
     3721 gggccccgtg agggccacgt tcgtgacgag cgcctggcgc gtgtagcgct cggaaaggag
     3781 cgccgtgccg tccggcgaga gcgacatccc gtaagacgtc acgcggcccc ccgccgacga
     3841 gtccagggaa agccacggcg cgatcgagag cggcacgctg atctgcggaa agaggtcgaa
     3901 gcggtcatac gtgccggcgg gctggttcgg accgcggtcc gtgtgcagca cgccgccctg
     3961 cgcgaggaag tcgaggaaga cgctctgccc gaagagcggc gtcggccgga ggcgggcctc
     4021 gagcgtcggg aggcgttcgc tcacgacgga gttgccgtcc gaaaagccga tcgcttcctg
     4081 gcgcgacagg cgcaggttca gggcccaagg gtccgggctc cacgtcagga acccctcgct
     4141 cccgatcgtg cgcgtcgagc cgagggcgaa gttccggttg aagtcctgga agaacgtcgg
     4201 gtccgagaag ttcagccacg tcgcgacccc gcgcaccttc ggggcgatgt cgtccgagac
     4261 gagccggccc gccgtctgcc agcgccacgc gtccacggtc gggtcctgga tcgtgtagta
     4321 cgtcccctcg aaccgcgtcc cggcggacgg ctggccgcgg acctcggcgc cgaacccgta
     4381 gtacttctcc gtgtagacgt cgccggagaa cgtcgcgtcc cacgagcggt cgatcgccca
     4441 gtagtacgac aggccgaagt agccgcctcg cgtcgagttg tagccgagcc ccgggacgag
     4501 gaagcccgac gcgcggtcgc ggagtgccgg ccagagaagg tacggcgtcc agagcagcgg
     4561 gaccccgccg agcgtgaacg tcgcgttctt gaggcgcgcg tagtcctcga gcgtgacgcg
     4621 gccgctcttg acggtgaact tccacgcggg gtcgtcgccg tcgcacgcgg tgaccgtgcc
     4681 ttccgtgagc gtgaagctct tcggacccac cttcgcgatc gtcgctccct tgaggtggaa
     4741 gcccccctcg aggtcgaccc ggccgtcgag gaggacgccg accttgtcgt tcaggtccag
     4801 gtcgaggcgc acgccgtaca tgcgcgagag gccttgcacg agcgtcacgt cgccctccgc
     4861 gacgaccgtg cgccgcttca ggctcccggt gacgcggcgc gcggtgaggc ggatgtcctg
     4921 gtactcgacc gtgacgaact tgccgggcgg agcgtcggcc gtgaagaggt cctcgtccac
     4981 gacggtctgc cgcccctgcg ggccgccttc ctcccccgcg tcgatcgcga tgcggatcga
     5041 gccgcccttt tcggggagct tgaacttcac ctcgaacccg cccttctcgc ggaagcgcga
     5101 ggcgaacggg tccgtgtcga aggaggactg ggagaacgcg cgccccgcgg ccaggaaccc
     5161 gagcaggacg aggccgattc gacgcatcac gtgacgcgcc gcaagacgga ggccagtcag
     5221 accttcatct cgggcacgtc gcccgaaacg tccaggggcg gctcggtggc ggcgcggatc
     5281 tcctcgacgg agacgcccgg agcgcgctcg acgaggacga ggcccccgcc tttcttcacg
     5341 tcgaggacgg cgagatccgt gacgatgcgg tgcacgcagc cgcgcccggt gagcggcagc
     5401 gtgcagcggt tcaggatctt cttctcgccg ttcttcgcga cgtgctccat cgtcacgacg
     5461 atccggcgcg tggccgcgac gaggtccatc gcgccgccca tgcccttcac gagcctgccc
     5521 gggatcatcc agttcgcgat cgagccctcg gcgtcgacct gcatcgcgcc gaggatgcag
     5581 aggtcgacgt ggccgccgcg gatcatcgcg aaggaatcgg cggacgagaa gaacgaggcg
     5641 cccgggatga cggtcaccgt ctccttgccc gcgttgatga ggtccgggtc gacttcggct
     5701 tcggtcggat acgggcccat ccctaggatg ccgttctcgg actggaggat gacctccatc
     5761 cccgcgggga tgaagttcga gacgagcgtc ggcatcccga tgccgaggtt cacgaccatc
     5821 ccgtcgcgca gctcctgcgc ggcgcggcgg atgatgccgt cgcggtcgag cttcgccatc
     5881 agcctttcct cacggtccgc cgctcgatcc gcttgacgcg cggcgcgacg acgacgcgct
     5941 ggacgaagat gccgggcgtg tcgacctcgt ccggatcgat ctcccccggc tcgacgagct
     6001 gctcgacctc ggcgatcgtg atcttccccg ccgtcgcggc catcgggttg aagttgcggg
     6061 ccgtcttccg gtagacgagg ttcccgagcc ggtcgccctt ccacgcggcg acgagcgcga
     6121 agtcgccgac gatgccgcgc tcgaggaggt actccttgcc gtcgaagacc ttcgtctcct
     6181 tgccctcggc cacgatcgtc ccgacgcccg tgggggtgta gaagcccgcg atcccggctc
     6241 cgccggcgcg catgcgctcg gcgagcgtgc cctgcgggca gaactcgagc tcgagctcgc
     6301 ccgcgaggta ctggcgctcg aactcggcgt tctcgccgac gtaggacgag agcatcttcc
     6361 ggatcttctt cttcccgagg aggagcccga ggccccagtc gtccacgccg cagttgttcg
     6421 acacgcacgt gaggcccgag acgtccgagg ccgcgagcgc gaggatgagg ttctccggga
     6481 tcccgcagag gccgaagccg cctacgacga gcgtcgcacc ggacggcacg tcgaggacgg
     6541 ccttcgccgc cgcctccgcc gggctcgctc ccctcacgac cttgtcgatc acggcccgat
     6601 tatcccgtca gagcgcttcg acgaccaccg cggtcgcttc tccgcccccg atgcagatgg
     6661 acgcgagacc gtacttcctc ttgcgcgcct tgagggcatg gacgagcgtc gcgaggatcc
     6721 gggcgccgga ggccccgatc gggtggccga gcgccaccgc gccgccgttc acgttcacct
     6781 tgttcgggtc gaggtcgagg tccttcacga acgccatcgc gacgaccgca aacgcctcgt
     6841 tcacctcgaa gagatcgatc gacgcgaggg ggagacccgc gcgctccgcg gccttcttcg
     6901 cggccgcgac gggcgccgtc gtgaaccact cgggctcctg ggcgaacgac gcctgggaca
     6961 ggatccgcgc gatcggcttg gccgacgccg cggccttctc gcccgcgaga acgagggccg
     7021 acgcgccgtc gttgatcttc gaggcgttcg cggcggtcac ggtgccttcc ttctggaagg
     7081 cgggcttgag cgatcccatc ttctcgagct tctcgagcgg cgtcgcgaac ggctcctcgt
     7141 cgcggtcgac caccgtcgcg cccttcttgc cctcgatcgt cacgggtgcg aactcggcgt
     7201 tgaagtcgcc cgactcgttc gcgcggcgcg cgcgctggta gctctcgagc gcgaacgcgt
     7261 cctgcgcctc gcgcgtgaac gcgtacttct cggcgcacat ctcggcgcag acgcccatgt
     7321 ggatgttctt gtacgggtcc caaaggccgt ccgagatcat tgagtccagg agctgcccgt
     7381 ggcccatccg gagcccgagg cggcccttgg ggagcaggta cggagcgttc gacatggact
     7441 ccatgccgcc cgcgaccgcg agctcgaact cgccgcaccg gagcgcgttc gcggcgtcca
     7501 tgacggcgcg catccccgag ccgcagacct tgtggaccgt cacggcgccc gtgcccttgg
     7561 cgagccctgc ggcgagaagc gcctggcggg ccggcgcctg gccctggccc gcctgcagca
     7621 cgttgcccat ccagacgtgc tcgacgcgcg cggcggcgac ctcggcgcgc tcgacggcgg
     7681 ccttgatcgc gacggccccg agcgcgtgcg cggggagcgt ggacagcgaa ccgaggaagg
     7741 agccgatcgg cgtgcgggcg gcggagagaa tgaagacgtc ttgactcatg atcctggttt
     7801 ctccgaggcg gcgggcgccg ccgaaggacg aggattgtat ccggagtgga cttcgaagag
     7861 gcgcgctcct acgatgagac tcgtgcgcgt cctcgcggtc gacttcggcg agaagcggat
     7921 cggcctcgcg gtttcggacg agaccgggcg cgtcgtcgtc ccggtgggcg ccgtcgcccg
     7981 gacgagcgac gcccgggcgg ccgcggacgt cgcgcgcgcc gcgaaggagc
//
LOCUS       DAOMFY010000174         8024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2799_length_8024_cov_6.377839, whole genome shotgun sequence.
ACCESSION   DAOMFY010000174 DAOMFY010000000
VERSION     DAOMFY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2799_length_8024_cov_6.377839"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PLB01_13235"
     CDS             <1..892
                     /locus_tag="PLB01_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQQ78311.1"
                     /translation="TSMVWRPDFEGRTANGHDAAGAVLGHARRGRPRAAGSMDTTLAD
                     YAKFMAGVLRGDIKDLVLLDVTPLSLGLETHGGIFVKLIERNSTIPTKNTQIFTTVAD
                     NQTTVEIHVLQGERDIAKENKSLGKFQLVGIPASAKGVPQIEVTFAIDSSGIVNVSAK
                     DLATGKAQGLQINPAGGLSQTEIEKIIEEAASNKAADAKRKEIRQLKNKLEGMIAAND
                     RIFREFGNLLQPDEKDRVDRTLANAREVLTGDERGPIDDAMIDMKGVSRILTQAMLYK
                     PESKERRVGGDSDKNAEKPV"
     gene            916..2037
                     /gene="dnaJ"
                     /locus_tag="PLB01_13240"
     CDS             916..2037
                     /gene="dnaJ"
                     /locus_tag="PLB01_13240"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQQ78312.1"
                     /translation="MSTSTRDYYEVLGVGRDASTEEIKAAYRKAALRHHPDKNPGDAA
                     AEERFKAAAEAYSVLSDPEKRARFDRFGPEGVRGAGGGPGFDPTQFVDFADILGDFFG
                     FGGESSRAARRNGEDLKADVTISFEDAAFGAEISIPLQRFEACGACRGRGGKGGASAA
                     TCPTCRGQGRVQFRQGFFAVARPCPECSGSGEKVKDPCPDCRGEGRVRRSRTLTIGIP
                     AGVDDGARLRLTAEGNSPRPGGRPGDLYVLVSVAPHEIFRRDGSDVILAWAVPFHLAA
                     MGGSMKVPTLHGEATFDLPAGTAAGRVFTLKGKGIPRLDGRGKGDQHVLVTIRVPKKM
                     THAQKEAVEKLAAAFDGEAGAPTKDEKGFFERLKEFLGT"
     gene            2086..2934
                     /locus_tag="PLB01_13245"
     CDS             2086..2934
                     /locus_tag="PLB01_13245"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQQ78313.1"
                     /translation="MAEAGVGATRIEIPAAQRGSFEEILFEGLGELQFSSTAAPDGRL
                     LLWVNSEDVPEALAALAALGASGLSTFKEESTDWVSASAALRTAVTVERYLFDPHEGV
                     LASVPPPGVRRLYLPAVRAFGTGSHESTRLAVRLLLAEELSGARVLDVGCGTGTLAFV
                     AALEGAVRVVAFDLDRDAAFATREQARANGIPRVYAFAGPIEALAPRALFDVIAANMI
                     HEEVAPLLPALRAHLTPGGRLLSSGQLVEREGEFEDLLRKTGFRCVRALRENEWLGTA
                     WVSSNE"
     gene            2960..3709
                     /locus_tag="PLB01_13250"
     CDS             2960..3709
                     /locus_tag="PLB01_13250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HQQ78314.1"
                     /translation="MLVPDLAEFLARLEEHQKISLKVEEREHVRSRRLRDGDEVVALD
                     GKGSRAAAVLVSGGSAIALLPSKQIFSPSPSLSSSSSSSSSLPGEPANRVVVALACAE
                     PARIEWAIEKGTECGAAGFVLLDAERSQRAHVAALAKRLPRLVRIAEEATKQCDRTLV
                     PFVEGPKSTGDFLRRVRGERVLVADAGGAQLAGPFPGAALAAIGPEGGFAPAEISLFE
                     ENSSLFFSLGPRILRLETAVVVALTRLVDPG"
     gene            3764..4711
                     /locus_tag="PLB01_13255"
     CDS             3764..4711
                     /locus_tag="PLB01_13255"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HQQ78315.1"
                     /translation="MSFENVKKIWMNGKLVDFADAKIHVFTHALHYGSGVFEGERCYN
                     TARGPAVFRLDDHLDRLFWSAKIYRMTIPYTLEELREATLEVVRANGFPACYIRPLVY
                     RGYGSLGVNPFPNPVDVTIGCYEWGKYLGAEALEKGVDVCCSSWMRIAPNTMPAMAKA
                     TANYANSQLIKMEAITNGYHEGIALDHNGRISEGSGENLFLVWKGRLYTPPLSASTLP
                     GITRLSVIQLSRELGFQMEEKTIPREMIYAADEMFFTGTAAEITPIRSVDRIPVGNGL
                     RGPMTEAIQKSFFDIVEGRVEDRYGWLTPVYAGVAAGRP"
     gene            4774..5334
                     /locus_tag="PLB01_13260"
     CDS             4774..5334
                     /locus_tag="PLB01_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78316.1"
                     /translation="MSSLIGTAFVAVLFVADPVPQPSSPISGLLSPPAACSSPEYRQF
                     DFWVGEWTVTNAKGQFAGENRVSVIQNGCAVQEHWTGADGGSGTSFNGYFDGDRKWHQ
                     TWLDSRGGRLDLAGGLVAGKMILRGQVPARGEPDQKVLHQISWERRSDGSVRQLWQSS
                     PDGGSSWTVVFDGTYVRKTAAGAAAR"
     gene            complement(5338..6114)
                     /locus_tag="PLB01_13265"
     CDS             complement(5338..6114)
                     /locus_tag="PLB01_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQQ78317.1"
                     /translation="MSTKLFLDLPAILAEHGRAALATVVSATGSTPREATARMLVLPG
                     GATRGTIGGGKFESLVIEDARKLLDGRGLPFTKRYDFLPEGPGTFGAVCGGTATVLLE
                     VLEHAPRLLVVGAGHCGRALARMASFAGWAVTVADERPEQLDPAAFPEGAGLVLVKED
                     YSDLPLPGPEDSVALVSRGHVTDGRAFRRLRGVPVAYLGMMGSHAKKKALFDELRGEG
                     WAEDELARAHSPIGLAIGAESPEEIAVAIVAELVKIRRGA"
     gene            complement(6111..6794)
                     /locus_tag="PLB01_13270"
     CDS             complement(6111..6794)
                     /locus_tag="PLB01_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQQ78318.1"
                     /translation="MSVSAVLLAAGASTRFGSPKMLAPVPPDGRPMLAHVVDTWRVAG
                     FAEIVVVLGSGAREIREKTEEDLLRMRKSRGGAGARDGRPDAGDGAAVRFVENLDWES
                     GMFSSVRAGLAAIRPESTHAALSPADLPFLRTSSLRAVLSATNLPEADSRTLLVPVCG
                     LRRGHPLLIPAFLVARVLSWPADDRLNRLFAEPDVKVLPLEGFDETVLLDVDRPADLA
                     AAVSSGARA"
     gene            complement(6806..7048)
                     /locus_tag="PLB01_13275"
     CDS             complement(6806..7048)
                     /locus_tag="PLB01_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78319.1"
                     /translation="MSAPTGFGDADPLDPALRCRHLRTKTMFVATGDWDEHAREHPST
                     SAAYWCLQVMGPVGPDGQPACLDDCRTGRGCFEGEG"
     gene            complement(7045..>8024)
                     /locus_tag="PLB01_13280"
     CDS             complement(7045..>8024)
                     /locus_tag="PLB01_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQQ78320.1"
                     /translation="DHPDVEKFVWSKAKEEKTSSEVKGAGTELIDRLKEYNLAPDRVI
                     DLRRLAAHSSIQRKSSTSVSIGALVTLSAVAEGVRADFPALADACLGAATPQIRNAAT
                     LAGNLCQRPRCWYFRSAEFQCLKKGGVECLAKSGENEFHAIFGNNTCAIVHPSAAGVA
                     LVALNARIETLAAGGGRTIPAADFFVRPEVDIFTENALMSGELIVEIHVEKPQGLRSA
                     YRKIKQKQAFDWPLADAAVAWREDGGVARDVRIVIGSAAPVPWRAKAAEALVEGKKID
                     AALAAKAGAAALEGATPLGKNAYKLPIVTAAVRRTLLASAGLADGTENA"
BASE COUNT         1271 a         2742 c         2807 g         1204 t
ORIGIN      
        1 caccagcatg gtctggcgcc cggacttcga ggggcggacc gccaacggcc acgacgcggc
       61 cggcgcggtc ctgggacacg cccggcgcgg gcggccccgg gcggcgggat ccatggacac
      121 caccctcgcg gactacgcca agttcatggc gggcgtcctg cggggcgaca tcaaggacct
      181 cgtcctcctc gacgtcacgc cgctctcgct cggcctcgag acgcacggcg gcatcttcgt
      241 gaagctcatc gagcgcaact cgacgatccc cacgaagaac acgcagatct tcacgacggt
      301 cgccgacaac cagacgacgg tcgagatcca cgtcctgcag ggcgagcgcg acatcgccaa
      361 ggagaacaag tccctcggca agttccagct cgtcggaatt ccggcctcgg ccaagggcgt
      421 gccccagatc gaggtcacgt tcgcgatcga ctcgtccggc atcgtgaacg tctcggcaaa
      481 ggacctcgcg acgggcaagg cccagggcct ccagatcaac ccggccggcg gcctttcgca
      541 gacggagatc gagaagatca tcgaggaggc ggcctcgaac aaggcggcgg acgccaagcg
      601 caaggagatc cgccagctca agaacaagct cgaagggatg atcgccgcca acgaccgcat
      661 cttccgggag ttcgggaacc tccttcagcc ggacgagaag gaccgcgtcg accgcacgct
      721 cgcgaacgcc cgggaggtcc tgacgggtga cgagcgcggg ccgatcgacg acgcgatgat
      781 cgacatgaag ggcgtctcgc gcatcctcac gcaggcgatg ctctacaagc cggagtccaa
      841 ggaacgccgc gtcggcggcg attcggacaa gaacgccgag aaaccggtct gaccgcggtc
      901 ccgtaggatc cctccgtgag cacgtctacc cgcgactact acgaagtact gggcgtcggc
      961 cgcgacgcgt cgaccgaaga gatcaaggcc gcgtaccgca aggccgccct caggcaccat
     1021 ccggacaaga acccgggaga cgccgccgcc gaggagcgct tcaaggccgc ggccgaggcc
     1081 tattccgtcc tctccgatcc ggagaaacgc gcgcgcttcg accgcttcgg gcccgagggc
     1141 gtgcgggggg ccggcggcgg ccccgggttc gacccgaccc agttcgtgga cttcgccgac
     1201 atcctcggcg acttcttcgg gttcggaggc gagtcctcgc gcgccgcgcg gcggaacggt
     1261 gaagacctca aggccgacgt cacgatttcg ttcgaggacg ccgcgttcgg cgccgagatc
     1321 tccattccgc tgcagcggtt cgaggcctgc ggagcctgcc ggggacgcgg cggaaaaggc
     1381 ggagcgtccg cggcgacgtg cccgacgtgc cgcggccagg gccgcgtcca gtttcgccag
     1441 gggttcttcg cggtggcgcg cccgtgcccc gagtgctcgg gctcgggcga gaaggtcaag
     1501 gacccctgtc cggactgccg cggagagggc cgggttcgca ggtcgcggac gctcacgatc
     1561 ggaatcccgg ccggggtcga cgacggcgcg cgcctgcgcc tcacggccga gggcaactcg
     1621 ccccggccgg gcgggcgccc gggcgacctg tacgttctcg tctccgtggc gccgcacgag
     1681 atcttccggc gagacggcag cgacgtgatc ctcgcgtggg ccgtgccgtt ccacctcgcg
     1741 gcgatgggcg gatcgatgaa ggtcccgacg ctccacggcg aggcgacgtt cgacctgccg
     1801 gccggcacgg cggcggggcg ggtcttcacg ctcaagggca agggcatccc gcggctcgac
     1861 ggccgcggca agggcgacca gcacgtcctc gtgacgatcc gcgtgccgaa gaagatgacg
     1921 cacgcgcaga aggaggccgt cgagaagctc gcggcggcgt tcgacggcga agccggcgcg
     1981 ccgacgaaag acgagaaggg cttcttcgag aggctcaagg agttcctcgg aacgtgacga
     2041 gcgacgcttc ggctcttgtc tcggcgcctt tctcagaagg tgaacatggc tgaggcgggg
     2101 gtcggcgcca cgcgaatcga gattccggcc gcccagagag ggtcattcga agaaatcctc
     2161 ttcgaagggc tgggcgagct ccagttctcc tcgaccgccg cgccggatgg tcgccttctt
     2221 ctctgggtca actccgagga cgtgccggag gcgctggccg ctctcgccgc gctcggcgct
     2281 tccggccttt ccaccttcaa agaagaatct accgactggg tgagcgcatc ggcagcattg
     2341 aggacggcgg tcacggtcga gcgctatctc ttcgaccccc acgaaggggt cctggccagc
     2401 gttccgcccc ccggcgtccg gcgtctctat ctccccgccg tccgcgcgtt cggcaccggg
     2461 tcgcacgagt cgacgcggct cgcggtgcgg ctcctcctcg ccgaagagct ctccggggcg
     2521 cgcgtcctcg acgtgggctg cgggacgggg acgctcgcat tcgttgcggc gctcgagggc
     2581 gccgtgcgcg tcgtcgcgtt cgacctcgac cgcgacgctg ccttcgcgac gcgcgagcag
     2641 gcgcgcgcga acgggattcc gcgcgtttac gccttcgccg ggccgatcga ggcgctcgcc
     2701 ccgcgagccc tcttcgacgt gatcgccgcg aacatgatcc acgaggaagt cgccccgctc
     2761 ctgccggcgc tccgcgcgca cctgactccg ggcgggaggc tcctctcctc cgggcagctc
     2821 gtcgaaaggg aaggagagtt cgaagatctt ctgagaaaga cgggcttccg ttgcgttcgt
     2881 gcgcttcggg agaacgaatg gctcgggacg gcgtgggttt cttcgaatga gtgagaaggc
     2941 cgaagaacgg ccgccacgcg tgctcgttcc cgatctggcg gagttcctcg caaggctcga
     3001 agagcaccag aagatttctt tgaaagtgga agagagagag cacgtgcgtt cgaggcggct
     3061 gagggacggc gacgaggtcg tcgcgctcga cggaaagggc tccagggccg cggcagtgct
     3121 cgtgagcggc ggctccgcca ttgccctatt accttctaag caaatcttct ctccctctcc
     3181 ctctctctct tcctcttcct cttcctcttc ttctctcccc ggcgagccgg cgaaccgcgt
     3241 cgtcgtcgcg ctcgcgtgcg ccgagcctgc gcggatcgag tgggcgatcg agaagggcac
     3301 cgaatgcggc gccgccggct tcgtcctgct cgacgccgag cgctcacagc gcgcgcacgt
     3361 cgcggcgctc gcgaagcggc tcccgcgcct cgtgcggatc gccgaggagg cgacgaagca
     3421 gtgcgaccgg acgctcgtgc ccttcgtgga aggtccgaag agtaccgggg attttcttag
     3481 aagggtaaga ggggagaggg tgctggtcgc agatgccggg ggcgcccagc ttgccggacc
     3541 gttccccggg gccgcgcttg ccgcaattgg ccccgagggc ggcttcgcgc cggccgagat
     3601 ctcactattc gaagaaaact cttctctttt cttctctctc ggcccccgca tcctccgcct
     3661 cgagacggcc gtggtcgtcg cgctcacgcg gttggtcgat ccgggctgat cgagcggccg
     3721 aatcttcgac actttcggat aggatcgcgc cccggaggcc ggaatgagtt tcgaaaacgt
     3781 caagaagatc tggatgaacg ggaagctggt ggatttcgcg gacgcgaaga tccacgtctt
     3841 cacgcacgcg ctccattacg gctccggcgt gttcgaaggc gagcgctgct acaacacggc
     3901 gcgcggcccc gcggtcttcc ggctcgacga ccacctcgac cgcctcttct ggtccgccaa
     3961 gatctaccgg atgacgatcc cctacacgct cgaggagctg cgcgaggcga cgctcgaggt
     4021 cgtccgcgcg aacggatttc cggcctgtta catccgaccg ctcgtctacc gcggctacgg
     4081 ctccctcggc gtgaacccgt tcccgaaccc ggtggacgtc acgatcggct gctacgagtg
     4141 gggcaagtac ctcggcgcgg aggcgctcga gaagggcgtc gacgtgtgct gctcgtcgtg
     4201 gatgcgcatc gcgccgaaca cgatgcccgc gatggcgaag gcgacggcga actacgcgaa
     4261 cagccagctc atcaagatgg aggcgatcac gaacgggtac cacgagggga tcgcgctgga
     4321 ccacaacggg cgcatctccg agggctcggg cgagaacctc ttcctcgtct ggaagggccg
     4381 cctctacacg ccgccgctct cggcgtcgac gctgccgggc atcacgcgac tgtccgtgat
     4441 ccagctctcg cgggagctcg ggttccagat ggaagagaag acgattccgc gcgagatgat
     4501 ctacgcggcc gacgagatgt tcttcacggg aaccgcggcc gagatcacgc cgatccgctc
     4561 cgtggaccgg atcccggtcg ggaacgggct gcgcggcccg atgacggagg cgatccagaa
     4621 gtcgttcttc gacatcgtcg agggccgcgt cgaggaccgc tacggctggc tcacgccggt
     4681 ctacgcgggc gtcgcggccg gccgcccgta accgcgcaca cacagcagaa cccgaggcat
     4741 gccccctgcg taccaccgtg ggaaggggga cgcatgtcga gcctgatcgg aaccgcgttc
     4801 gtcgccgtgc tcttcgtggc ggaccccgtg ccgcagcctt cgtccccgat ttcaggcctt
     4861 ctgtccccgc ccgcggcgtg ttcgtccccg gagtaccgcc agttcgactt ctgggtcggc
     4921 gagtggacgg tgacaaacgc gaagggacag ttcgccggcg agaaccgggt gagcgtcatc
     4981 cagaacggat gcgccgtcca ggagcactgg acgggcgccg acggcgggtc gggcaccagc
     5041 ttcaacggct acttcgacgg cgaccggaaa tggcaccaga cctggctcga ttcccgcggc
     5101 gggcgtctcg acctcgcggg aggcctcgtt gccggaaaga tgatcctccg tgggcaggtt
     5161 cccgcgcgtg gcgagccgga ccagaaggtg ctccaccaga tctcctggga acgccgctcg
     5221 gacgggagcg tgcgccagct ctggcagtcc tccccggacg gcggctcgtc gtggaccgtc
     5281 gtgttcgacg ggacgtacgt gaggaagacc gccgcgggcg ctgcggcgcg gtgacgctca
     5341 ggccccgcgc cggatcttga cgagctcggc gacgatcgcg accgcgatct cctccggcga
     5401 ctcggctccg atcgcgaggc cgatcgggct gtgcgcccgg gcgagttcgt cctcggccca
     5461 gccttccccg cgcagctcgt cgaagagcgc cttcttcttc gcgtggctcc ccatcatccc
     5521 gagatacgcg acggggacgc cccgcagccg ccggaacgcg cggccgtccg tcacgtggcc
     5581 gcgggagacg agcgcgacgg agtcctccgg cccgggcagc ggaagatcgg agtagtcctc
     5641 cttcaccagc acgaggccgg cgccctcggg gaacgcggcg ggatccagct gctcgggtct
     5701 ttcgtccgcg acggtcacgg cccaccccgc gaaagacgcc atgcgtgcga gcgcccgccc
     5761 gcagtggccg gcgcccacga cgagcagccg cggcgcgtgc tcgagcacct cgagcaggac
     5821 cgtcgccgtc ccgccgcaca cggcgccgaa cgtgccgggt ccctcgggga gaaagtcgta
     5881 tctcttcgtg aacggaagcc cgcggccgtc gagaagcttc ctcgcatcct cgatgacgag
     5941 cgactcgaac ttccctccgc cgatcgtgcc gcgcgtggcg cccccgggca gaacgagcat
     6001 ccgcgcggtg gcctcgcgag gggtcgagcc cgtggcggag acgacggtcg cgagcgcggc
     6061 gcggccgtgc tccgcgagga tcgcaggaag gtcgaggaag agcttcgtgc tcacgcgcgg
     6121 gctccggagg acacggcggc ggcgaggtcg gcgggccggt cgacgtcgag gaggaccgtc
     6181 tcgtcgaagc cttcgagcgg gaggaccttc acgtccggct cggcgaagag ccggttgagc
     6241 cgatcgtcgg ccggccagga gaggacgcgc gcgacgagga acgccgggat caggagcggg
     6301 tgcccgcggc gcaagccgca gacggggacg aggagcgtgc gggagtccgc ctcgggcagg
     6361 ttcgtcgcgg agagaacggc ccgaagagaa gatgttctga gaaagggaag atcggcgggg
     6421 gagagcgcgg cgtgcgtcga ctcgggtcga atcgcggcga gcccggcgcg gacggacgag
     6481 aacatcccgg attcccagtc gaggttctcg acgaaacgga cggcggcgcc gtcgcccgcg
     6541 tcggggcgcc cgtcgcgtgc gccggcgcct cctctgctct tcctcattct gagaagatct
     6601 tcttcggtct tctcccgaat ctctcgcgcc cccgagccga gcaccacgac gatctcggcg
     6661 aagcccgcga cccgccacgt gtcgacgacg tgcgcgagca tcgggcgccc gtccggcggc
     6721 acgggggcga gcatcttcgg cgagccgaac cgcgtggagg ccccggcggc gaggaggacg
     6781 gcggagacgc tcacgggacc tgcgttcagc cttcgccctc gaagcagccg cggcccgtgc
     6841 gacagtcgtc gaggcaggcg ggctgcccgt ccggacccac gggtcccatg acctggaggc
     6901 accagtacgc cgcggacgtg gacgggtgct cgcgcgcgtg ctcgtcccag tcgcccgtgg
     6961 cgacgaacat cgtcttcgtg cggaggtggc ggcagcgcag cgccgggtcg agggggtcgg
     7021 cgtcgccgaa tcccgtgggg gcgctcatgc gttctccgtg ccgtcggcga ggcctgccga
     7081 cgcgaggagc gtccgccgga cggccgcggt cacgatcggc agcttgtacg cgttcttccc
     7141 gagcggcgtc gcgccctcga gcgccgccgc accggccttc gcggcgagcg ccgcgtcgat
     7201 cttcttccct tcgacgagcg cctcggccgc cttcgcccgc cacggcacgg gcgcggcgga
     7261 gccgatcacg atccggacgt cccgggcgac gcctccgtct tcacgccacg cgacggccgc
     7321 gtcggcgaga ggccagtcga acgcctgctt ctgcttgatc ttgcggtacg ccgagcgcag
     7381 cccctgcggt ttctcgacgt ggatctcgac gatcagctca ccgctcatca gcgcgttctc
     7441 cgtgaagatg tccacctcgg ggcgaacgaa gaaatccgcg gcgggaatcg tgcgtccgcc
     7501 tcccgcggcg agcgtctcga tgcgggcgtt cagggcgacg agcgcgacgc ccgcggccga
     7561 ggggtggacg atcgcgcacg tgttgttccc gaagatcgca tggaattcgt tctcgccgga
     7621 cttggcgagg cactcgacgc cgcccttctt gaggcactgg aactcggccg agcggaagta
     7681 ccagcagcgc gggcgctggc agaggttgcc cgcgagcgtc gccgcgttgc ggatctgggg
     7741 cgtcgccgcg ccgaggcacg cgtcggcgag agcggggaag tccgcacgca cgccctcggc
     7801 gaccgcgctg agtgtgacga gagcgccgat ggagaccgag gtagaagatt ttctttgaat
     7861 ggaggaatgg gcggcgaggc gcctcagatc gatgacgcgg tccggtgcga ggttgtattc
     7921 cttgagtcgg tcgatcagtt ccgtcccggc tcccttaacc tcagaagaag tcttctcttc
     7981 tttcgccttt gaccagacga atttttccac atccgggtga tcga
//
LOCUS       DAOMFY010000175         7972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2833_length_7972_cov_8.595806, whole genome shotgun sequence.
ACCESSION   DAOMFY010000175 DAOMFY010000000
VERSION     DAOMFY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2833_length_7972_cov_8.595806"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1693)
                     /locus_tag="PLB01_13285"
     CDS             complement(38..1693)
                     /locus_tag="PLB01_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ78321.1"
                     /translation="MEALRLTRRDAIASLVGAPALAALASALAGCSRKDVRALEFPGG
                     FAVPDATLGHLLRGGDLLVRPVARTERIGTAIVGGGVSGLSAAWRLARSGDGDFRVYE
                     MEGSPGGTAVSGGSDVSLFPWGAHYVPVPAFRNPALEAVLEEVGALVGRDADGAPEWA
                     EEMLCREPEERLYYGNRWHPGLYPYEGASRTDVEQLNRFRAAMDAFAGRKDGKGRRAF
                     AIPTRLSSDDADLLALDRMTMDEWLDANGFTSKRLRWLAEYGCRDDFGTNLGRISAWA
                     GIHYNASRLPGEDEDEEPSDFLTWPEGNGRLVAHLAKSASGKLGGNALVFDVAPSGRP
                     GTPVRLRYFDAKTKEVVAVEAKAVILALPKFAARHVFAPWRAAPPAWLDALSWAPWLV
                     ANVTLSGRPRETGHPLAWDNVLYDSRSLGYVVATHQTGKDHGSTVFTYYLPLTDEEPV
                     KARALLLAAEWKDLAAAVLADLSRAHADLPSLVTRMDFARWGHAMLRPTPGFLSSPVR
                     RLTPEGGVHFAHADAAGLPLFEEAQDAGVRAAESILAARGVHT"
     gene            complement(1675..3219)
                     /locus_tag="PLB01_13290"
     CDS             complement(1675..3219)
                     /locus_tag="PLB01_13290"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HQQ78322.1"
                     /translation="MSADRAEGRAAGSARGVTPVYLTAFAIASCGLVYELVAGALASY
                     LLGDSVTQFSLVIGVYLSSLGLGAFLSRYVVRGVARRFVDAEIAVALIGGLSAPALFF
                     AFSSPRWFPPVFYAVVVLIGTIVGLEIPLLMRLLKDAVEFRDLVSQVLTFDYLGSLAA
                     SLLFPLVLLPRLGLTRTSVLFGLVNVAVAFWSLHLFRDRIGSPAGLYVRASLTAALLV
                     AAFVLADHLTAAAEETQYVDEVIVAKTTAYQRIVVTKNRAGFQLFLNGHLQFSSVDEY
                     RYHEALVHPAFAAAGGRGRRVAVLGGGDGLAVREVLKHPEVESVTLVDLDPGMTALAR
                     THPLFVELNGGSLASPKVRIVNDDAMVWIAASKDLFDVVIVDFPDPNSYAVGKLYTSR
                     FYTLLARRLDPEGVVSVQSTSPLFARRSFWIVDATLRAAGFATKAYHVAVPSFGEWGF
                     VLAAKKPFTVASRGLPDGLRALSAQLLPTLFVFGPDTGPVDSPVNRLNDQVLVRTYER
                     EWKRFD"
     gene            complement(3239..3463)
                     /locus_tag="PLB01_13295"
     CDS             complement(3239..3463)
                     /locus_tag="PLB01_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HQQ78323.1"
                     /translation="MSWWNLHGHQVISTIVFSVVGLFLFGIFFATLPKVLPFSLKKEM
                     EEDQNVALAIVIGSIVIAMAIIIAAAVTGG"
     gene            complement(3463..3630)
                     /locus_tag="PLB01_13300"
     CDS             complement(3463..3630)
                     /locus_tag="PLB01_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78324.1"
                     /translation="MRRFFQVFGSLLLLGYAWAAFTGWEMGGAVKHETLPPGARNAGG
                     YRTYHFWSGGK"
     gene            complement(3640..5715)
                     /locus_tag="PLB01_13305"
     CDS             complement(3640..5715)
                     /locus_tag="PLB01_13305"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HQQ78325.1"
                     /translation="MSARAAQCPNCGAEVLFSAGSSLVTICRQCRSAVARRGPIIESI
                     GVVAELVPTSSPFKVGMRSRGTKTVKPFTIVGRLQLSTGEGTWDEWHVALGDGGFAWL
                     AEAQGGFWMMLPLKPPPVPDWTQLAPGQPLDLGAYGRYAVAEVRQATYVSAEGDLPFV
                     APPGSVFRYADISGADGSLGTLDYGDDPGLDGFYVGRKLELAELGIEGLTAWKDRKTS
                     AKAQALNCPACGGALELKDPANTVRVACSHCGSILGGRDETSPPDKFAVIRKLKAVPF
                     KPSIPPGSSGVLNGHPYTVLGAVQKETTSDGTTYLWVEYLLKEQKSEAYHWLAESNGH
                     FTLLEPVPGGGVEATARYATYNGARYRIFSRSRASVRAVVGEFYWAVKKGETTTATDY
                     VAPPRMLSSESGDSESAWTEGAWVPQADVAAAFPSAVLPSPSGVGAAQPWPHEGTSHL
                     WWKTARILALAAVVVFAAAKVTAPKRVVFDHTYDLVDPDKMIYAERGSAAAAGGWAQQ
                     SAPAPQNPVAAREAREEAEAAQRVILSEPFETTRTANLEAVIWSPTDNNWIGVGVALI
                     AEGTGDVRSFGLVSDRYHGVDGGESWSEGRQSRTVWVSRVPPAKWVARLDPETERGKA
                     PPQFRIRLTSGVPHLSHLIWTLVFLLVPPFLLVFSRLSWEGRRWAESDFTSAGGERTD
                     SDSGSDSDD"
     assembly_gap    5741..5840
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5841..>7972)
                     /gene="acnA"
                     /locus_tag="PLB01_13310"
     CDS             complement(<5841..>7972)
                     /gene="acnA"
                     /locus_tag="PLB01_13310"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HQQ78326.1"
                     /translation="LGWGVGGIEAEAALLGQPQSMLIPDVIGFKLVGTLPAGATATDL
                     VLTVTQMLRKKGVVGKFVEFFGPGVAGLALTDRATIANMAPEYGATMGFFPVDAETIK
                     YLKFTNRPAKTVALAEAYCKKQGLFRTNDSPEPVFTDTLTLNLKDVEPSLAGPKRPQD
                     RVPLKDSKKIFRGSLKNFLEKECATLDQTAIGRWIADGGQPAASASLSSPSKNSSSSS
                     SSLGPLVKTAPVADGRVTYDIPQGAVVIASITSCTNTSNPSVMLGAGLLAKKAVERGL
                     SVKPWVKTSLAPGSKVVTDYLKDAGLMPYLEAVGFHLVGYGCTTCIGNSGPLPEAVSE
                     AVKTGDLVVAGVLSGNRNFEGRINPQVKMNYLASPPLVVAYALAGEMDKDLTTEPIGT
                     DRSGAPVYLKDIWPTQQEVLDAIRTSVKPAHFQKAYGEVFDGDASWQGLKIPKGRTYV
                     WEESSTYVRKPPFFTNIPKVPAPAKDLAGARALVMLGDSVTTDHISPAGNIAKNSPAA
                     KYLVAHGVAPADFNSYGARRGNHEVMMRGTFANIRLKNLLIPGSEGNVTIHFPSGEQM
                     SIYDASMKYQASRIPLVVLAGAEYGTGSSRDWAAKGTMLLGVKAVIAKSFERIHRSNL
                     AGMGVLPLQFEAGQDAASLGLSGQETFAVAGIAEGLSPKKKLTVTATDTEGKKKTFTA
                     VCRLDTPNEVDYYLHGGILHFVLRQLASA"
BASE COUNT         1221 a         2645 c         2891 g         1115 t
ORIGIN      
        1 gcgacgcccg cggcggcgag cgccgcgcgg gagcgcgtca cgtgtgaacg ccccgcgccg
       61 cgaggatcga ctcggcggcg cgcacccccg cgtcctgcgc ctcctcgaaa agcggcaggc
      121 ccgccgcgtc cgcgtgcgcg aagtggacgc cgccttcggg cgtgaggcgg cggacggggc
      181 tcgagaggaa gccgggggtc gggcggagca tcgcgtgccc ccagcgtgcg aagtccatcc
      241 gcgtcacgag agacggcagg tccgcgtgcg cgcgcgagag atcggcgagg acggcggccg
      301 cgaggtcctt ccactccgcc gcgagaagga gcgcgcgcgc cttcacgggt tcctcgtcgg
      361 tgagcgggag gtagtacgtg aagaccgtcg agccgtggtc cttgcccgtc tggtgcgtcg
      421 cgaccacgta acccagagaa cgcgagtcgt agaggacgtt gtcccaggcg agggggtgcc
      481 ccgtctccct gggccgcccc gagagcgtga cgttcgcgac gagccacggc gcccaggaga
      541 gggcgtcgag ccatgcgggt ggcgccgcgc gccacggggc gaagacgtgc cgggcggcga
      601 acttcgggag cgcgaggatc acggccttcg cctccacggc gacgacctcc ttcgtcttcg
      661 cgtcgaaata ccggagccgc accggcgtcc cgggccgtcc cgaaggcgcg acgtcgaaaa
      721 cgagggcgtt gccgccgagc ttccccgacg cggatttcgc gaggtgggcg acgagacggc
      781 cgttcccttc gggccacgtc aggaagtccg acggctcctc gtcctcgtcc tccccgggca
      841 ggcgcgacgc gttgtagtgg atgcccgccc aggcggagat ccgcccgagg ttcgtgccga
      901 agtcgtcacg gcagccgtac tcggcgagcc agcgcagccg cttcgacgtg aagccgttcg
      961 catccagcca ctcgtccatc gtcatgcggt ccagcgcaag gaggtcggcg tcgtccgacg
     1021 acaggcgcgt cgggatcgcg aacgcgcggc ggcccttccc gtcctttcgg cccgcgaacg
     1081 cgtccatcgc ggcgcggaag cgattcagct gctcgacgtc cgtgcgcgac gcgccctcgt
     1141 acgggtagag cccgggatgc cagcggttgc cgtagtagag gcgctcctcg ggctcgcggc
     1201 agagcatctc ctcggcccat tcgggggccc cgtccgcgtc gcgcccgacg agagcgccca
     1261 cctcctcgag gacggcctcg agcgccgggt tgcggaacgc cggcacgggg acgtagtggg
     1321 cgccccacgg aaagaggctg acatcgctgc cgcccgagac ggcggtcccg ccgggcgagc
     1381 cttccatctc gtacacgcgg aagtcgccgt cgcccgaacg cgcgaggcgc cacgcggccg
     1441 agaggcccga gacgcctccg cccacgatcg cggtcccgat gcgctcggtc cgcgcgacgg
     1501 ggcgcacgag gaggtcgcct ccgcggagga ggtggccgag cgtggcgtcg ggcacggcga
     1561 agccgcccgg aaactcgagg gcgcggacgt ccttccgcga gcagccggcg agcgccgatg
     1621 cgagggccgc gagcgcgggg gccccgacga gcgacgcgat cgcgtcgcgg cgcgtcagtc
     1681 gaagcgcttc cactcgcgct cgtacgtgcg gacgaggacc tggtcgttga ggcggttcac
     1741 cggggagtcc acggggcccg tgtcggggcc gaagacgaag agagtgggga ggagctgcgc
     1801 cgaaagagcg cgcaggccgt ccggcaggcc ccgggacgcg accgtaaacg gcttcttcgc
     1861 cgcgaggacg aacccccact cgccgaagga cgggacggcc acgtggtacg ccttcgtcgc
     1921 gaagccggcg gccctgagcg tcgcgtcgac gatccagaag ctcctccgcg cgaagagcgg
     1981 tgacgtggac tggaccgaca cgacgccctc ggggtcgagc cgccgcgcga ggagcgtgta
     2041 gaagcgcgac gtgtagagct tcccgaccgc gtagctgttc gggtcgggga agtccacgat
     2101 cacgacgtcg aaaaggtcct tcgaagcagc gatccagacc atcgcgtcgt cgttcacgat
     2161 gcggaccttc ggtgacgcga gcgagccgcc gttcagctcg acgaagagcg ggtgcgtcct
     2221 cgcgagcgcc gtcatcccgg ggtcgaggtc cacgagcgtg acgctctcga cctcggggtg
     2281 cttgaggacc tcgcgcacgg cgaggccgtc gccgccgccg aggaccgcca cgcgccgccc
     2341 ccgccccccg gcggccgcaa acgccgggtg gacgagcgct tcgtggtagc ggtactcgtc
     2401 cacggacgag aactgcaggt gcccgttcag gaagagctgg aagcccgcgc ggttcttcgt
     2461 cacgacgatg cgctggtaag cggtcgtctt cgcgacgatg acctcgtcga cgtactgggt
     2521 ctcctcggcc gcggccgtga ggtggtcggc gaggacgaac gcggccacga gaagggcggc
     2581 cgtgagcgac gcgcggacgt agaggcccgc cgggctcccg atgcggtccc ggaagaggtg
     2641 cagcgaccag aacgcgaccg cgacgttcac gaggccgaag aggacggacg tccgcgtgag
     2701 gccgaggcgg ggaaggagga cgagcgggaa gaggagcgag gccgcgagcg agccgaggta
     2761 gtcgaacgtg aggacctgcg acacgaggtc gcggaactcg acggcgtcct tcaggagacg
     2821 catgaggagc gggatctcga ggccgacgat cgtcccgatc agcacgacga cggcgtagaa
     2881 gacgggcggg aaccaccggg gagaggagaa cgcgaagaag agggccgggg ccgagaggcc
     2941 gccgatcagc gcgaccgcga tctcggcgtc cacgaagcgc cgcgcgacgc cgcgcacgac
     3001 gtaccgcgag aggaacgccc cgagcccgag ggacgagagg tagacgccga tgacgaggga
     3061 gaactgcgtg accgagtcgc cgaggaggta actggcgagc gcgcccgcga cgagctcgta
     3121 gacgagcccg cacgaggcga tcgcgaaggc cgtcaggtag acgggcgtca cgccgcgggc
     3181 ggacccggcg gcgcggcctt ccgcgcggtc cgcgctcacg cgcactccgg gaggatcgtc
     3241 agccgcccgt gacagccgcg gcgatgatga tcgccatcgc gatcacgatc gagccgatga
     3301 cgatggcgag ggcgacgttc tggtcttcct ccatctcctt cttgagggaa aaggggagga
     3361 ccttcggcaa ggtcgcgaag aagatcccga acagaaagag cccgacgacc gagaacacga
     3421 tcgtcgagat cacctgatgc ccgtgcaggt tccaccagct catcactttc ctccgctcca
     3481 gaagtggtag gtacggtagc cgcccgcgtt gcgcgcgccc ggcggcaggg tttcgtgctt
     3541 cacggcgccg cccatctccc agcccgtgaa cgcggcccac gcgtatccga ggaggaggag
     3601 cgagccgaag acctggaaga accgtctcat cgtgtctccc taatcgtccg agtccgatcc
     3661 ggaatccgag tccgtgcgct cgccgccggc cgacgtgaaa tcgctctccg cccagcgccg
     3721 cccttcccac gagaggcgcg agaaaaccag caggaaaggc ggcacgagga ggaacacgag
     3781 cgtccagatc aggtgcgaaa gatgcggcac gccgctcgtg agccggatcc ggaactgcgg
     3841 cggagccttc ccgcgctcgg tctcggggtc gaggcgcgcg acccatttcg ccggcgggac
     3901 gcgcgagacc cacacggtgc ggctctggcg gccctcgctc cagctctcgc cgccgtcgac
     3961 gccgtggtac cggtccgata cgaggccgaa agagcgcacg tcgccggttc cctcggcgat
     4021 gagggcgacg ccgacgccga tccagttgtt gtccgtcggc gaccagatca cggcctcgag
     4081 gttcgccgtg cgcgtcgtct cgaacggctc gctcaggatc acgcgctggg cggcctcggc
     4141 ttcctcgcgg gcctcgcgcg cggcgacggg attctgggga gcgggcgcgg actgctgcgc
     4201 ccagccgccg gcggcggcgg ccgaaccgcg ctcggcgtag atcatcttat ccggatccac
     4261 gaggtcgtag gtgtggtcga acaccacccg tttcggcgcg gtgaccttcg cggccgcgaa
     4321 gacgacgact gcggcgagcg cgaggatccg ggccgttttc caccacaggt ggctcgtccc
     4381 ctcgtgcggc cacggctgcg ccgcgcccac gccggaagga gaaggcagga ccgccgatgg
     4441 gaacgccgcg gccacgtcgg cctgcgggac ccaggcgcct tccgtccacg cggattcgga
     4501 gtcgccggat tcgctcgaga gcatccgcgg cggcgcgacg tagtccgtcg cggtcgtcgt
     4561 ctcgcccttc ttgaccgccc agtagaactc gccgacgacg gcgcgcacgc tcgcgcggct
     4621 gcgcgagaag atccggtagc gcgcgccgtt gtacgtcgcg tagcgggccg tcgcttcgac
     4681 gccgcctccc gggacgggct cgaggagcgt gaagtggccg ttcgactcgg cgagccagtg
     4741 gtacgcctcg gacttctgtt ccttgaggag gtactcgacc cagagatacg tcgtcccgtc
     4801 ggacgtcgtc tccttctgga cggcgccgag cacggtgtac gggtggccgt tcaggacacc
     4861 cgaagagccc ggcgggatcg acggcttgaa cggcaccgcc ttgagcttgc ggatgaccgc
     4921 gaacttgtcg ggcgggctcg tctcgtccct gccgccgagg atcgatccgc agtgcgagca
     4981 cgcgacgcgg acggtgttcg cggggtcctt gagctcaagg gcgccgccgc aggcggggca
     5041 gttcagcgcc tgcgccttcg cggaggtctt gcggtccttc caggccgtga ggccctcgat
     5101 cccgagctcg gcgagctcga gcttccggcc cacgtagaag ccgtcgaggc ccgggtcgtc
     5161 gccgtagtcg agcgtgccga gcgagccgtc ggcgcccgag atgtccgcgt accggaagac
     5221 ggagccgggc ggcgcgacga acgggaggtc cccctccgcc gagacgtacg tggcctgccg
     5281 cacctcggcg accgcgtagc ggccgtacgc gccgaggtcc agcggctggc cgggcgcgag
     5341 ctgcgtccag tcgggcacgg gaggcggctt gagcggcagc atcatccaga agccgccctg
     5401 cgcctcggcg agccaggcga atccgccgtc tcccagcgcg acgtgccact cgtcccacgt
     5461 gccctcgccc gtcgagagct ggaggcgccc gacgatcgtg aagggcttga cggttttcgt
     5521 gccgcggctg cgcatcccga ccttgaacgg cgaggacgtc gggacgagct cggccacgac
     5581 gccgatcgac tcgatgatcg ggcctcgccg cgcgaccgcg gaacggcact gccggcagat
     5641 cgtcacgagg gacgacccgg ccgagaagag gacctccgct ccgcagttcg ggcactgcgc
     5701 cgcgcgcgcg ctcaaggtgg ggagatctta gtcgagaacg nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn gcgctcgcga gctgcctcag gacgaagtgg aggatgcctc
     5881 cgtggaggta gtagtccacc tcgttcggcg tgtcgaggcg gcagacggcc gtgaaggtct
     5941 tcttcttccc ctccgtgtcc gtcgccgtga ccgtgagctt cttcttcggg gagaggccct
     6001 cggcgatgcc ggcgaccgcg aacgtctcct gcccggagag cccgaggctc gccgcgtcct
     6061 ggcccgcctc gaactggagc gggagcacgc ccatcccggc gaggttcgag cggtggatgc
     6121 gctcgaagct cttcgcgatg acggccttca cgccgaggag catcgtgccc ttcgcggccc
     6181 agtcgcgcga ggagcccgtg ccgtactcgg cgcccgcgag aacgacgagc gggatcctcg
     6241 aggcctggta cttcatcgac gcgtcgtaga tcgacatctg ctcgccgctc gggaagtgga
     6301 tcgtgacgtt gccctcgctg cccgggatca ggaggttctt gaggcggatg ttcgcgaacg
     6361 tgccgcgcat catgacctcg tggttgccgc ggcgggcgcc gtaggagttg aagtccgcgg
     6421 gcgcaacgcc gtgcgcgacg aggtacttcg cggccgggga gttcttcgcg atgttgccgg
     6481 ccggggagat gtggtccgtc gtcacggagt cgccgagcat gacgagggcg cgcgccccgg
     6541 cgaggtcctt cgcgggcgcg gggaccttcg ggatgttcgt gaagaagggg ggcttcctca
     6601 cgtacgtcga ggactcctcc cacacgtacg tccggccctt cgggatcttg aggccctgcc
     6661 agctcgcgtc gccgtcgaag acctctccgt aggctttctg gaagtgcgcg ggcttgaccg
     6721 acgtccggat cgcgtcgagg acctcctgct gcgtcggcca gatgtccttg aggtacacgg
     6781 gcgcgccgct gcggtcggtg ccgatcggct cggtcgtgag gtccttgtcc atctcgcccg
     6841 cgagcgcgta cgcgacgacg agcgggggcg acgcgaggta gttcatcttg acctgcgggt
     6901 tgatgcggcc ctcgaagttg cggttgcccg agagcacgcc cgcgacgacg aggtcgcccg
     6961 tcttcacggc ctcggacacg gcttcgggca gcgggcccga gttgccgatg cacgtcgtgc
     7021 agccgtagcc gacgaggtgg aagccgacgg cctcgaggta gggcatgagg ccggcgtcct
     7081 tcaggtagtc cgtgacgacc ttcgagccgg gcgcgagcga cgtcttgacc cagggcttta
     7141 cggagagccc gcgctcgacg gccttcttcg cgaggaggcc ggcgccgagc atgacggacg
     7201 ggttcgacgt gttcgtgcac gacgtgatcg acgcgatcac gacggcgccc tgcgggatgt
     7261 cgtacgtgac gcggccgtcg gcgacgggcg cggtcttcac gagaggaccg agagaggaag
     7321 atgaagagga agagttcttg gaaggtgaag agagggacgc cgacgcggcg ggctggccgc
     7381 cgtccgcaat ccatctgccg attgcagtct ggtccagcgt cgcgcattcc ttttccaaga
     7441 aattcttcaa actccctctg aagatcttct tgctgtcctt gagcggcacg cggtcctgcg
     7501 ggcgcttggg gcccgcgagg gagggctcga cgtccttgag gttgagcgtc agcgtgtccg
     7561 tgaagacggg ttcgggcgag tcgttcgtgc ggaagaggcc ctgcttcttg cagtaggcct
     7621 cggccagcgc gacggtcttc gcgggccggt tcgtgaactt caggtacttg atcgtctcgg
     7681 cgtcgacggg gaagaagccc atcgtcgcgc cgtactcggg cgccatgttc gcgatcgtgg
     7741 cgcggtccgt gagcgcgagg cccgcgacgc cggggccgaa gaactcgacg aacttcccga
     7801 cgacgccctt cttgcggagc atctgtgtga ccgtgaggac gaggtcggtc gccgtcgcgc
     7861 ccgcgggcag cgtcccgacg agcttgaacc cgatgacgtc cgggatgagc atggactgcg
     7921 gctggccgag gagcgcggcc tcggcctcga tcccgcccac gccccagccg ag
//
LOCUS       DAOMFY010000176         7965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2836_length_7965_cov_7.394437, whole genome shotgun sequence.
ACCESSION   DAOMFY010000176 DAOMFY010000000
VERSION     DAOMFY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2836_length_7965_cov_7.394437"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLB01_13315"
     CDS             complement(<1..898)
                     /locus_tag="PLB01_13315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ78327.1"
                     /translation="MTLAAGTKIGPYEIVSPLGAGGMGEVYRARDPRLDRVVALKVLP
                     EDFFEEREWRTRFEREAKALAAINHPNIAAVYSFEEVSGRHLLVQELLEGETLRDRLL
                     AGPLPMRKAVEFGLQVAQGLAAVHEKGIVHRDLKPENLFITTDGRVKILDFGLARYTP
                     HAGADDTRSPTLAKPTDPGVVLGTVGYMSPEQVRGKPADARSDIFSFGAILYEMLTGQ
                     RAFQGDSPADTMSGILTKEPPDLADLNPGIPPGLERLVRHCMEKSVEERFHSAHDLAF
                     DLGALSTASGAALPLRPAGASRR"
     gene            complement(895..3627)
                     /locus_tag="PLB01_13320"
     CDS             complement(895..3627)
                     /locus_tag="PLB01_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ78328.1"
                     /translation="MTLAPGTKLGSYEILAPIGAGGMGEVYRAKDARLAREVAVKVLP
                     EEFFEGEEKRQRFEREARLLAALNHPGIAAIYSFEEIPSSASSSSRHILVMELLEGET
                     LRAKLAGGAIAPRKAVDYALQMAQGLAAAHDRGIVHRDLKPENVFVTRDGRVKILDFG
                     LARQAAPKLGEDETSAPTAARHTDPGTVMGTVGYMSPEQVKGLPVDHRSDLFAFGAIL
                     HEMLSGKGAFKRDTPPETLTAILREEPADLSGPSRAVDPALERLVLHCLEKSPEERFQ
                     SARDLAYALSTLSSSSGALSGAMGAVGARVAGRRVRLAIPAAVAAGAILGAIGARFVL
                     RPKASAPPEFQVLTYAGSDGQPSVSPDGRTIAFVSSRDGRPRVWVKQVVGGAEAVLTE
                     GPDSQPRVSPDGSSVLFLRSSAAESSVYRAALVGGEARRVVGPAVAADWSPDGKEIVF
                     LRTSAAGGTIDSSLRRIALDGTNERAIGEVPGRLLALPRWSPDGRTIAALESPGTGTA
                     TPKLVLFPVDGSAPTRVDTPETRAVLGYAWNGDGRRIVFLGGAASDANSRSRTAKVFL
                     KDIRTGTSRAVLSGIDFGGAVDVFGNGALVLVSVALRSNLREIPFGGGSAQERWLTRG
                     GSVDRQPIYAPDGEWVAFTSNRSGNQDIWELSTKTGAVHRLTEDPSDDFDPAFTRDGK
                     SLIFSSNRGGHFEIWMAARDGGGARRVTDDGVDAENASATPDGKWLVYSSGNPDKRGI
                     WKIRPDGTGATRLVAGPVILPEISPDGRLVSFVSVSGPDGSTSVSGGANSAIGAVRLD
                     DGAPVPFEAIATGAIANRGRHRWTPDGGSLVFLTGDRKETLGLTAQPFIPGRDTTPAR
                     RPVAGFTPGSLSESFGISPDGSRMTISEFQVSFGLLLAEGVDGVVARPSRGGRP"
     gene            complement(3624..6284)
                     /locus_tag="PLB01_13325"
     CDS             complement(3624..6284)
                     /locus_tag="PLB01_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ78329.1"
                     /translation="MSLAPGTKLGPYEILAPLGEGGMGEVYRAKDARLGREVALKVLS
                     ARVATSASARERFERESKAVAALSHPNILALYDVGLEGETAYAVTELLEGQTLRKALQ
                     PGSLPIRKALDVAMQVGKGLVAAHEKGIIHRDLKPENLFVTRSGHVKILDFGLAKLSL
                     AESDDSGPAGISNEGHTEPGRILGTVGYMSPEQARGQAADARSDIFGLGAVLYEMLSG
                     KRAFHKDSGIDTLSAILKEEPPDLVLTNQLVPPGLDRLVRHCLEKDPALRFQTARDLV
                     FDLETLLGESLSGSLGIAPPQEQASPRKRLRLFLAAALVAGLAAGAAWTLAIRVRSGT
                     SGGALRPTFQRLTSQPGGETKPALSPDGETVAFVSTAAGNRDIWIQRVGSDKAINLTA
                     DSSVDEMDPAFSPDGSLIAFHSDRDGGGLFVMGALGESVRRVTTGGFSPDWTPDGKEI
                     VYADEEITSPLLRSRSKLWAANAASGQKRLLLSTDGVEPAVSPHGLRVAFWGLKGDTA
                     QRDVYTIPLAGLKDGESAVPVTDDAAVDLSPFWSPDGKTLYFGSDRGGSFNLWRVPID
                     EESGRTRGEPEPVTLPTTWAGIFPGSFRGARARFRVAFTAPAELMTIEKVTLDPATLA
                     TAGPPAILRRGSAMFLDLELSPDGSTLATRTQGATENLCVLPANGTSLRQITRDSFRN
                     RWARWSPDGQRIFFYSNRSGAYGVWTIKPDGSDLTRVPALPDASWAAPVPSPDGALIA
                     LGRPSTGHVVVVPLTSSPGPGATPAPPLEIEGKWAWDWSPDGRRLLVARRGAVSASGV
                     LLCGVPSGQCEVVAPNAEQGRFFPDGRHVGYVEHGEIHVRDLSTGGSGLLVSASADSP
                     ITRFCVSRDGRALYLLRDVSEGDIWVATFP"
     gene            complement(6281..>7965)
                     /locus_tag="PLB01_13330"
     CDS             complement(6281..>7965)
                     /locus_tag="PLB01_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78330.1"
                     /translation="GGRRWAPAAAFALALAAVAASYAAGHWKGKRSIAAVASFAQLTF
                     RQEPIFKARLAPDGKTAVYSAAPSGNTPEIFALRTDYPGASPRSLGAGQLLSISSRGE
                     LALLTRAQYKGHSLFEGTLARMPLEGGAPREILDGVREADWTPDGSDVAVIRDVGGKD
                     RLEFPAGKVLAETGGYFSELRFSPRGDRIAFFQHPFKFDDRGEVAVVDLAGKKTILSE
                     GYWGEEGLAWSPTGDEVMFSAGNAYNNFRVYGVTLGGKRRIALESAGGLTIHDIRADG
                     RWLATRDDFFREMPVLAPGQGRERDLSWLDLSDPAALTSDGKTLLFMEESGSVGVNYA
                     VCLRRTDGSPVVRLGEGAALDLSRDGKWALAAVPTTPPQLVLYPTGAGEPRKLERGGL
                     VSYESGQFFPDTRRVLVCGHEEGKGVRCYVQDVGGGKPLPVTPEGTSQGFVSPDSRQV
                     LVKASGGALVLYPVDGGEARPVPGASPGDAVIRWSADGRSVLVSRNGEVPARIERLDV
                     ATGRREPVRTIGPADLTGVLSIGPFAFTDDLKSYAYACRRTSSHLFLVEGAR"
BASE COUNT         1193 a         2788 c         2794 g         1190 t
ORIGIN      
        1 ctcggcggga ggcgcccgcg gggcggagcg gcaacgccgc tcccgacgcg gtggaaagcg
       61 ccccgagatc gaacgcgagg tcgtgcgcgg aatggaagcg ctcctccacg ctcttctcca
      121 tgcagtgccg gacgaggcgc tcgagtccgg gcgggatgcc tggattcagg tcggcgagat
      181 cgggcggctc cttcgtcagg atcccgctca tcgtgtcggc cggcgaatcc ccctggaaag
      241 cccgctggcc cgtgagcatc tcgtagagga tcgcgccgaa tgagaagatg tcgctgcgag
      301 cgtcggccgg ctttccccga acttgctcgg gcgacatgta accgaccgtc cccaggacca
      361 cacccgggtc cgtgggtttc gccagtgtgg gcgaccttgt gtcgtcggcg cccgcgtgcg
      421 gcgtgtatcg ggctagaccg aagtccagga tcttcacgcg gccgtccgtc gtgatgaaga
      481 ggttctcagg cttcaagtcc ctgtggacga tccccttctc gtgaaccgca gcaagtccct
      541 gcgcgacctg gagtccgaat tcgacggctt tccgcatcgg gaggggcccc gccaggagac
      601 ggtcgcggag cgtctcgccc tcgagaagct cctgcacgag gagatgacgc cccgagactt
      661 cttcgaatga gtagacggcg gcgatgttcg ggtggttgat cgcggccagc gccttggctt
      721 ccctctcgaa gcgggttctc cactctcttt cttcgaagaa gtcttccggc aagaccttca
      781 gggccaccac ccggtcgagg cgcgggtccc tcgcccggta gacctccccc atcccgcccg
      841 ctccgagcgg gctcacgatc tcgtacgggc cgatcttcgt gccggcggcg agcgtcacgg
      901 cctccctccg cgcgaaggcc gcgcgacgac tccgtcgacg ccttccgcga ggaggaggcc
      961 gaaggagacc tggaactcgc tgatcgtcat gcgcgagccg tcgggcgaaa tgccgaagga
     1021 ctccgaaagg gagcccggcg tgaagcccgc cacgggccgc cgcgccggcg tcgtatccct
     1081 gccggggatg aagggctggg ccgtgaggcc aagagtctct tttcggtcac ccgtcaggaa
     1141 cacaagcgag ccgccgtccg gcgtccagcg gtggcggccg cggttggcga tcgcgcccgt
     1201 cgcgatggcc tcgaacggga cgggcgcgcc gtcgtcgagg cgcacggccc cgatcgcgct
     1261 gttcgctccg cccgagacgc tcgtcgatcc gtccggcccg ctcacgctga cgaacgacac
     1321 gagacggccg tcgggcgaga tctccgggag gatcacaggc cccgcgacca gccgcgtcgc
     1381 gcccgtcccg tcgggccgga tcttccagat gccgcgcttg tccgggttgc cggacgagta
     1441 gacgagccac ttcccgtccg gtgtggcgga tgcgttctcc gcgtcgactc cgtcgtccgt
     1501 gacgcggcgc gcgccgccgc cgtcgcgcgc cgccatccag atctcgaagt gcccgccacg
     1561 gttggagctg aagatgaggc tcttgccgtc gcgcgtgaac gcagggtcga agtcgtcgga
     1621 ggggtcttcc gtcagacggt ggacggcgcc cgtcttcgtg gagagctccc agatgtcctg
     1681 gtttccgctc cggttggacg tgaacgcgac ccactcgccg tccggagcgt aaatgggctg
     1741 ccggtcgacg ctgccgcccc gggtgagcca tcgctcctgc gcgctcccgc ctccgaacgg
     1801 gatttcgcgg aggttggacc ggagtgcgac cgaaacgagg acgagagcgc cgttcccgaa
     1861 gacgtcgacg gccccgccga agtcgatccc cgagagcacg gcgcgggagg ttcccgtccg
     1921 gatgtctttc aggaagacct tcgccgtccg ggaacggctg ttggcgtccg acgcggcgcc
     1981 gcccaggaag acgatgcgcc ggccgtcccc gttccaggcg tatccgagaa cggcgcgcgt
     2041 ctccggcgtg tcgacacgag tcggcgccga gccgtcgacg gggaacagca cgagtttcgg
     2101 cgtggccgtt ccggtccccg ggctctcgag agcggcgatc gttctgccgt cgggcgacca
     2161 gcgcggcagc gccagaagcc gcccgggcac ctctccgatg gcccgctcgt tcgttccgtc
     2221 cagcgcgata cgacggaggg acgagtcgat cgtgccgccc gccgcgctcg tcctgaggaa
     2281 gacgatctcc ttcccgtccg gcgaccagtc ggcggccacc gcgggaccga cgacacgccg
     2341 cgcttcgccg cccacgaggg cagcccggta gacggacgac tcggcggcgg acgatcggag
     2401 gaaaaggacg gacgagccgt ccggagagac gcgaggctgg gagtccggac cctcggtgag
     2461 caccgcctcg gcgccgccca cgacctgctt gacccagacg cggggccggc cgtcgcggga
     2521 agagacgaag gcaatcgtcc ttccgtccgg cgacaccgac ggctggccgt cgctccccgc
     2581 gtacgtgagg acctggaatt cgggcggcgc cgaggccttc ggcctcagaa cgaaccgcgc
     2641 cccgatggcg ccgagaatcg cgcccgcggc aaccgccgcc ggaattgcga gccggacgcg
     2701 ccggcccgcc acccgggcgc ccacggcgcc catcgcgccg ctgagcgcgc cggacgagga
     2761 cgacagcgtg gagagcgcgt acgcgaggtc gcgcgcggac tgaaaacgtt cctcagggct
     2821 cttctcgaga cagtggagga cgagccgctc gagcgcggga tcgacggctc ggctcgggcc
     2881 ggagagatcg gcgggttcct cgcgcaggat cgcggtcagc gtctcgggag gcgtgtcgcg
     2941 cttgaacgcg cccttgcccg agagcatttc gtggaggatc gctccgaagg cgaagaggtc
     3001 cgaccggtgg tcgaccggga ggcccttgac ctgctccggc gacatgtaac cgaccgtgcc
     3061 catcacggtg ccgggatcgg tgtggcgcgc ggccgtgggc gcgctcgtct cgtcctcgcc
     3121 gagcttcggc gcggcctgtc gggcgaggcc gaagtcgagg atcttgacgc gcccgtctct
     3181 cgtgacgaag acgttctcgg gtttcaggtc ccggtggacg atccccctgt cgtgagcggc
     3241 ggcgagcccc tgagccatct gaagggcgta gtcgaccgcc ttgcgcggcg cgatcgcgcc
     3301 gcccgccagc ttcgcgcgga gcgtctctcc ctcgagcagc tccatcacga gaatgtggcg
     3361 cgacgaagag gaagcggacg aggggatttc ttcgaatgag tagatggcgg caattccggg
     3421 atgattgagc gctgcgagca gcctcgcctc ccgctcgaac ctctgcctct tctcttcacc
     3481 ttcgaagaat tcctccggca ggaccttgac tgccacctct ctcgcgagcc tcgcgtcctt
     3541 cgcccggtag acctccccca tcccccctgc gccgatgggc gcgaggattt cgtaggagcc
     3601 gagtttcgtc ccgggcgcga gcgtcacggg aacgtcgcga cccagatgtc gccctcgctc
     3661 acgtcccgga ggaggtagag ggcccgcccg tcgcgggaga cgcagaaacg ggtgatcggg
     3721 gagtccgcgc ttgcggagac gagcagcccg gaacccccgg tggagaggtc gcgcacgtga
     3781 atctcaccgt gttccacgta gccgacgtgc cgcccgtccg ggaagaaccg cccctgctcg
     3841 gcattcggcg cgacgacttc gcactggccg gagggcactc cgcacaggag cactccggac
     3901 gcggacacgg cgcccctgcg cgcaacgagc aggcggcgcc cgtcgggcga ccagtcccag
     3961 gcccactttc cctcgatctc gagaggcggc gcaggcgtcg caccgggtcc cggagacgag
     4021 gtgagcggga cgacgacgac gtggcccgtc gacggccttc cgagcgcgat cagggctccg
     4081 tccggcgacg gcaccggggc cgcccagctc gcgtcgggca gcgcggggac acgcgtcagg
     4141 tcgctcccgt cgggcttgat cgtccagacg ccgtacgcgc cgctccggtt ggaatagaag
     4201 aagattcgct gtccgtccgg cgaccagcgt gcccagcggt tccggaaaga gtcgcgtgtg
     4261 atctggcgga ggctcgtccc gttcgcgggg aggacgcaga ggttttcggt ggctccctgc
     4321 gtccgcgtcg cgagggtcga gccgtcgggc gaaagctcga ggtccaggaa catcgccgag
     4381 ccgcgccgga ggatggccgg aggacccgcg gtcgcgaggg tcgcggggtc gagcgtcacc
     4441 ttctcgatcg tcatgagctc cgccggtgcc gtgaaggcga cgcggaagcg ggcgcgggcg
     4501 ccgcggaagg agccggggaa gatgccggcc cacgtcgtcg gaagcgtcac gggctcgggc
     4561 tcgccgcggg tccgcccgga ctcctcgtcg atcggaaccc gccagaggtt gaaactcccc
     4621 ccgcggtcgc ttccgaagta gagcgtcttg ccgtcgggcg accagaacgg gctgaggtcg
     4681 acggcggcgt cgtcggtcac ggggacggcg ctctctccgt ccttgagacc cgcaagcggg
     4741 atcgtgtaga cgtcacgctg ggcggtgtcg cccttgaggc cccagaacgc cacacgcagc
     4801 ccgtggggcg agaccgcggg ctcgacgccg tccgtcgaga ggagaagcct cttctggcca
     4861 gaagccgcgt tcgccgccca gagcttgctc cggctgcgca agagcggcga ggtgatctcc
     4921 tcgtccgcgt agacgatctc cttcccgtcc ggcgtccagt ccggagagaa gccgcccgtc
     4981 gtcacgcggc gcaccgattc gcccagagcg cccatcacga agagccctcc gccgtcgcgg
     5041 tctgaatgga aggcaatgag ggatccgtcg ggcgagaacg ccggatccat ctcgtcgacg
     5101 gaggaatccg cggtgaggtt gatcgccttg tcgctcccga cgcgctggat ccagatgtcc
     5161 ctgtttcccg ctgccgtgct cacgaacgcg acggtctctc cgtcgggcga caaggcgggc
     5221 ttggtctctc cgccgggttg cgacgtcagt cgctggaacg tcggccggag agctccgccc
     5281 gaggttccgc tccgcacgcg gatcgcgaga gtccacgcgg ccccggcggc gagccccgcg
     5341 acgagcgccg ccgcgaggaa gaggcgcagc cgcttccggg gcgacgcctg ctcctgaggc
     5401 ggggcgatcc cgagcgaccc ggacagcgat tcgcccagga gagtctcgag gtcgaacacg
     5461 aggtcccgcg ccgtctggaa acggagcgcc ggatctttct cgaggcagtg gcgcacgagg
     5521 cggtcgaggc ccggcggaac gagctggttc gtgaggacga gatcgggggg ctcttccttg
     5581 aggatcgccg agagcgtgtc gatccccgaa tccttgtgga acgctctctt gcccgagagc
     5641 atctcgtaga ggaccgctcc gaggccgaag atgtccgagc gcgcatccgc cgcctggccc
     5701 cgcgcctgct cgggcgacat gtacccgacg gtcccgagga tgcggccggg ctcggtgtgc
     5761 ccctcgttgg agatgcccgc cgggccggag tcgtcggact cggcgagcga gagtttcgcg
     5821 agcccgaagt cgaggatctt cacgtggccg ctgcgcgtca cgaagaggtt ttcgggcttg
     5881 aggtccctgt ggatgatccc cttctcgtgg gcggcgacga ggcccttccc aacctgcatc
     5941 gcgacgtcga gagcttttcg tatcgggagg cttcccggct gcagcgcctt ccggagcgtc
     6001 tgcccttcga ggagctcggt cacggcgtag gccgtctcgc cctcgagtcc cacgtcgtag
     6061 agggcgagga tgttcggatg cgaaagagcg gcgacggcct tcgactcgcg ctcgaaccgc
     6121 tcgcgtgcgg acgcgctcgt cgcgacgcgc gccgagagca ccttgagcgc gacctcgcgg
     6181 cccagccgcg cgtccttcgc ccggtagacc tcccccatcc cgccctcgcc cagcggcgcg
     6241 aggatctcgt acggtccgag cttggtgccg ggagcgagac tcatcgcgca ccctccacga
     6301 gaaagagatg agaagaggtc cgccggcagg cgtaggcgta gctcttcaaa tcgtcggtga
     6361 acgcgaacgg cccgatgctc aggacgccgg tgaggtcggc gggcccaatc gtgcgcaccg
     6421 gctcccgtct tcccgttgca acgtccagcc gctcgatacg cgccggaact tcgccgtttc
     6481 gggagacgag caccgagcgg ccgtccgcgc tccaccggat gaccgcgtcg ccgggactcg
     6541 cgcccggcac gggcctggcc tctcccccgt ccacgggata gaggaccagg gcgcctccgc
     6601 tcgccttgac gaggacctgt ctgctgtccg gagacacgaa tccctggctc gtgccttccg
     6661 gcgtgacagg caggggcttt ccgcccccca cgtcctggac gtagcaccgg acgcccttgc
     6721 cctcctcgtg accgcaaacg agaacccgcc gggtgtccgg gaagaactgt ccggactcgt
     6781 agctgacgag gccgccgcgc tcgagcttgc gcggctcgcc ggctcccgtg ggatacagga
     6841 cgagctgcgg aggcgtcgtc ggcacggccg caagcgccca cttcccgtcg cgggagaggt
     6901 cgagcgcagc cccttcgccg aggcgcacca cgggcgagcc atccgtccgc cgcaggcaca
     6961 ccgcatagtt cacgccgacg ctcccgctct cctccatgaa gagcaacgtc ttcccgtccg
     7021 acgtcagcgc cgccggatcg gagaggtcga gccaggagag atcgcgctcg cgcccctgac
     7081 cgggagcgag caccggcatc tcgcggaaga aatcgtcccg cgtcgcgagc cagcgaccgt
     7141 cggcccggat gtcgtggatc gtgagcccgc ccgcgctctc gagcgcgatt cgccgcttgc
     7201 ctccgagggt gacgccgtaa acgcggaagt tgttgtaggc gtttcccgcg gagaacatga
     7261 cctcgtctcc ggtcggcgac caggccaggc cctcctcgcc ccagtagccc tctgagagga
     7321 tcgtcttctt ccccgcgaga tccacgacgg cgacttcgcc gcggtcgtcg aacttgaacg
     7381 ggtgctggaa gaacgcaatc cggtctccgc gcggagaaaa gcggagctcg ctgaagtagc
     7441 cgcccgtctc ggcaaggacc tttccggccg ggaattccag ccggtccttt ccgccgacgt
     7501 cgcggatcac ggcgacgtcg ctgccgtcgg gagtccagtc ggcctcgcga acgccgtcca
     7561 ggatttcccg cggcgcgccg ccttccagcg gcattcgcgc gagcgtgccc tcgaagaggc
     7621 tgtgcccctt gtactgggcg cgcgtgagaa gcgcgagctc tccacgggac gagatcgaga
     7681 gcagctgtcc ggcgccgagg gagcgcgggc tcgcgcccgg gtagtccgtg cggagcgcga
     7741 agatctccgg cgtgtttccg gacggcgccg cgctgtagac ggccgtcttc ccgtccgggg
     7801 cgagccgggc cttgaagatc ggctcctgcc ggaacgtgag ctgcgcgaag gacgcgacgg
     7861 cggcgatcga gcgctttccc ttccagtgcc cggccgcgta agacgccgcc acggcggcga
     7921 gggcgagggc aaacgcggcc gcgggcgccc accgccggcc acccc
//
LOCUS       DAOMFY010000177         7962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2838_length_7962_cov_7.322626, whole genome shotgun sequence.
ACCESSION   DAOMFY010000177 DAOMFY010000000
VERSION     DAOMFY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_7962_cov_7.322626"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLB01_13335"
     CDS             <1..819
                     /locus_tag="PLB01_13335"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQQ78331.1"
                     /translation="ALDAARRARFEADLALERRRADQDHLVETCRAEFGCLPGELPAL
                     EKTEEELALAEDVFEAQLAEDVVQKTLALERLGPVNHAALEEYDVEHARFVELSGQKA
                     DLEASLAQILETIKTINATSSERFTEAFAAVNANFGAVFQRLFHGGTASMHLLDEDDP
                     LDSGLEIMAQPPGKRNQTIGLLSGGEKALTAIALLVAIFKYKPSPFCILDEVDAPLDE
                     ANIDRFTSLLVELSEETQFVLITHNKRTMETAQALYGVTQEEPGVSKLVSVRFD"
     gene            complement(816..1904)
                     /locus_tag="PLB01_13340"
     CDS             complement(816..1904)
                     /locus_tag="PLB01_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78332.1"
                     /translation="MRNDLIPALDPAPVPGPPALLVVLWIATFFLHLLAVNAALGGAL
                     FSVLLPPSMPGASRRAAALFFTEINSWALSLAITFAIAPLLFMQVLTGRFFYTASVLV
                     APVWLGLLLLLMLAYYLNWIAKFRLRAGKGAAAVLAISATCSLAIAAIQTSVHLISVQ
                     PSVWSAVLDRPSLVLGDPTFIPRLLHFVLAAVSMAAVLPAAFPRTLADAAPGAARFGL
                     FAALVLTGAQFAVGLWLLLALPRTVLSGLMTGGAAKLAPLALGILVGLALLGLLVRAV
                     GEPGNARLARVVLGHFAAAALFMIVTRHQVRDLYLAGARAGERLVVEPQWSVLALFFG
                     VFILCAGLSAWALFRAATDRPGPGEAAA"
     gene            complement(1894..3246)
                     /locus_tag="PLB01_13345"
     CDS             complement(1894..3246)
                     /locus_tag="PLB01_13345"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQQ78333.1"
                     /translation="MDYPIWDLAVGGGVLIGAIAILHVVVSHFAVGGGLVMAWAETSL
                     GRRPDPALRALLKRASLMLILVSTVFGAISGVAIWFVIGLVHPAATSALIHTFVWGWA
                     IEWAFFLLEVVTALAWYATWDRVRPRTHRLLIVLYAFAAYMSLVVIQGILGFMLTPGR
                     WLETRGFWDGFLNPTYLPGIVFRTGVCLLLAGAYLMFAALRERDRAALARLAGLLGRV
                     MAVGVAVAAAGLFWWKAALPPDVAANFLGGKPLIASLGTGWRHLFIALAASAVLALLA
                     ALLPRTVRWPQAVLALVAAFGLLGGYERIREGARKPFIVRGYMFSNGILVSEIATLNR
                     EGVLSKARWAAHEAGDDAVSKGRAVYRAECASCHTLDGYLSIRKRTASADADFLTLFL
                     TALRDDGSKWTATPPQKADYPFMPPFVGTDEELQALVAYLDSLKPTGAVKTAEATRAQ
                     "
     gene            3448..4305
                     /gene="coxB"
                     /locus_tag="PLB01_13350"
     CDS             3448..4305
                     /gene="coxB"
                     /locus_tag="PLB01_13350"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQQ78334.1"
                     /translation="MSQEAPLHRRVRRGASRTARSLAVLVPFLASAAALAGPSGPYAP
                     PRDVSTEGHRVTWLFWYTTGLGIVAFTLVCASLVYIVARYRARAGHRAVYDKGTDRRS
                     LVVTGSLALLVFLSIDLVLVHRSRADIEEYLFKWPTSADTVRIEVMPQQWVWNFRYAG
                     ADGVFNTADDVVTLNEMWIPKGRPVFLNLKAKDVIHSFYVPNFRIKQDANPGHVTRMW
                     FQAKETGSMEIACSQLCGWAHYKMKADLTVMENDAFERWYAEAQGDAKRRFDPADTEA
                     QWGWEWVAK"
     gene            4302..6095
                     /locus_tag="PLB01_13355"
     CDS             4302..6095
                     /locus_tag="PLB01_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQQ78335.1"
                     /translation="MTTRRPRLAYAGPDFHAEQHIHPDPDSFLRRWVFSLDHKVIAKQ
                     FLWLGLAFLAIGGTMAMMIRWQLGHPGTAFPVLGELIWPQSGGVMPPQSYSSFFTMHG
                     TIMIFFAITPILIGAFGNFCIPLMIGARDMAFPTLNMLSFWTMALSSGILVASFFVPG
                     GSASGGWVSYPTLSTLVGQPAPGQTLWIVAIFLAGSSSIMGAVNYITTVIRLRAPGMG
                     YFDMPLTVWGLWLTAILNALFVPVIAAGMLLLFLDRVFGSHFFVAGMAVTKGGGDPIL
                     YQHLFWIFGHPEVYILILPAWGVVSDVLSFFSRKPAFGYKATALSMTAITVLSMVVYG
                     HHMFVVGMSPLLSESFMTLTMLISMPSAVFFLNWLGTMWRGSIRFTTPMLFSMGVVFV
                     FGLGGLTGLHLGAIGTDVYLHDSYFVVGHFHLTMAASVLLGAFSAIYFWFPKLFGKMM
                     NERLGKLHFWLTIVPIVLVFGAQLVIGYAGMQRRLYNLDSYTFLQHLQPLNLFITRAA
                     LFLGATQFLFVANFLSSLISGAKASENPWEVGTLEWTVPSPPPHHNFDEIPLVLHGPH
                     EFSHPGVTGKDWLAQNEPLEAEPVPLGAVGS"
     gene            6092..6682
                     /locus_tag="PLB01_13360"
     CDS             6092..6682
                     /locus_tag="PLB01_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HQQ78336.1"
                     /translation="MNAAALTRVAAREESRRTSLVGMVMALGSWTMLFVSLFFSYAVL
                     RLRAGEWPPDGLEPLPRLLPFLNTLVLAASSLVLHAGVRSAAAVRPRALLSALRRTLA
                     LGSLFLALQLAVWIPLWRSGFRMDSGVFGSIFYGLTVFHALHVLAGLVALLVLVPGAA
                     KGRWVSGAQSPVRLTAMFWHFVDAVWVLMFVTIYLL"
     gene            6714..7646
                     /locus_tag="PLB01_13365"
     CDS             6714..7646
                     /locus_tag="PLB01_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQQ78337.1"
                     /translation="MKVGTRVAAGGVGAFLGALLLLGSGCSGPGARFSKSQTLGGKRV
                     SARRLNHGRELFVTYCAACHGLEGDGKGPAAVGLRPPPRNFKQGQFKFAAVASGQLPN
                     DEDLLRIVQKGLHGSAMLPWNDVPERDLLDIIQYLKTLSPKWAEKTAGEPIVPSPDPY
                     GAARKAEAVTRGMKVYHGLAQCLSCHPAYETPETIQAASLELSKRETTLRPDPYHASP
                     KESDYGVKIMPPDFTRDHVRSGEALTDIYRTVASGIGGTAMPTWKGALPEEDIWAMAY
                     YVRSLIDIKNTPEADARRAKLVAMAPPETTTAAK"
     gene            7692..>7962
                     /locus_tag="PLB01_13370"
     CDS             7692..>7962
                     /locus_tag="PLB01_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78338.1"
                     /translation="MAAPVFASGGSAAARVVGGAAPSPSPSKKSPSSFLLPQRLAARL
                     TGSPWFWAVFVFAVFAVPVFRSVTRPLPAAPPVLGELPAFALTDQQ"
BASE COUNT         1262 a         2810 c         2685 g         1205 t
ORIGIN      
        1 gccctcgacg cggcgcgccg cgcgcgcttc gaggccgacc tcgcgctcga gcgccgccgc
       61 gccgaccagg accatctcgt cgagacctgc cgcgccgagt tcggctgcct gcccggagag
      121 ctgcccgcac tcgagaagac cgaggaggag ctcgcgctcg ccgaggacgt cttcgaagcg
      181 cagctcgccg aggacgtcgt ccagaagacg ctcgctctcg agcggctcgg tccggtcaac
      241 cacgccgccc tcgaggagta cgacgtcgag catgcccgct tcgtcgagct ctccggccag
      301 aaggccgatc tcgaggcctc cctcgcgcag atcctcgaga cgatcaagac gatcaacgcg
      361 acgtcgtcgg agcgcttcac ggaggcgttc gcggccgtca acgcgaactt cggcgccgtc
      421 ttccagcggc tcttccacgg cggcacggcc tccatgcacc tcctcgacga ggacgacccg
      481 ctggactcgg gcctcgagat catggcgcag ccccccggca agcggaacca gacgatcggc
      541 ctcctgtcgg gcggcgagaa ggcgctcacg gccatcgcgc tgctcgtggc gatcttcaag
      601 tacaagccgt cgccgttctg catcctcgac gaggtcgacg ccccgctcga cgaggcgaac
      661 atcgaccgct tcacgtccct cctcgtggag ctgtccgagg aaacgcagtt cgtcctcatc
      721 acgcacaaca agaggacgat ggagaccgcc caggcgctct acggcgtgac gcaggaagag
      781 cccggggtct cgaagctcgt cagcgtccgg ttcgactagg ccgcagcctc ccccgggccc
      841 ggccggtccg tcgccgcgcg gaagagcgcc cacgcggaga gccccgcgca caggatgaag
      901 acgccgaaga agagagcgag aacgctccac tgcggctcga cgaccagccg ctcgcccgcg
      961 cgcgctccgg cgaggtagag gtcgcggacc tggtggcggg tgacgatcat gaagagcgcc
     1021 gccgccgcga agtggccgag gacgacccgc gcgaggcgcg cgtttccggg ctccccgacg
     1081 gcgcggacca ggagaccgag gagggcgagc ccgaccagga tcccgagggc gagcggcgcc
     1141 agtttcgcgg ctccaccggt catgaggccc gacaggacgg tcctcgggag ggcgagcagg
     1201 agccagaggc ccaccgcgaa ctgagccccg gtcagaacga gggccgcgaa gaggccgaaa
     1261 cgggccgccc cgggcgccgc gtccgcgagc gtccggggaa aggcggcggg gagaaccgcg
     1321 gccatcgaga ccgccgcgag cacgaagtga aggaggcggg gaatgaacgt ggggtcgccg
     1381 agcacgagcg acgggcgatc gaggacggcg ctccagaccg acggctggac cgagatgaga
     1441 tggaccgagg tctggatcgc cgcgatcgcg agcgagcagg tcgccgagat cgcgaggacc
     1501 gccgccgcgc ccttccccgc ccggagccgg aacttcgcga tccagttgag gtagtaggcg
     1561 agcatgagca gcaggagaag cccgagccag acgggggcga cgaggaccga cgccgtgtag
     1621 aagaagcgtc cggtgagcac ctgcatgaag aggagcggcg cgatcgcgaa cgtgatcgcg
     1681 agcgagagcg cccacgagtt gatctccgtg aagaagagag cggcggcccg ccgcgaggcg
     1741 cccggcatcg aaggcggcag gaggaccgag aagagcgcgc cgccaagcgc cgcgttgacc
     1801 gcgagcaggt ggaggaagaa cgtcgcgatc cagaggacga cgaggagcgc cgggggtccg
     1861 gggaccggcg ccgggtcgag ggccgggatc aggtcattgc gcacgggtcg cctccgcggt
     1921 cttcaccgcc ccggtgggct tcagggagtc gagataggcc acgagggcct ggagttcttc
     1981 gtcggtcccg acgaagggcg gcatgaacgg gtagtccgcc ttctgcggcg gcgtcgcggt
     2041 ccacttcgac ccgtcgtcgc gcagcgccgt gagaaacagt gtcaggaagt ccgcgtcggc
     2101 cgacgccgtc cgcttgcgaa tcgagaggta tccgtcgagc gtgtggcacg aggcgcactc
     2161 ggcgcggtag accgcccggc ccttcgacac ggcgtcgtcc cccgcctcgt gcgccgccca
     2221 acgcgctttc gagaggacgc cctcccgatt cagtgtcgcg atctcggaaa cgaggatgcc
     2281 gttcgagaac atgtagcccc ggacgatgaa gggcttgcgc gccccctcgc ggatccgctc
     2341 gtagccgccg aggaggccga aggccgcaac gagcgcgaga accgcctgcg gccaccggac
     2401 cgtgcgcggc aggagcgccg cgaggagtgc caggacggcg gaggccgcga gggcgatgaa
     2461 gaggtgccgc cagcccgtcc cgagcgacgc gatcagcggc ttgccgccca ggaagttcgc
     2521 cgccacgtcg ggcgggagcg cggccttcca ccagaagagc ccggcggccg cgacggcgac
     2581 gccgaccgcc atgacccggc cgaggagccc ggcgaggcgc gcgagcgccg cgcggtccct
     2641 ctcccggagc gccgcgaaca tgaggtacgc gcccgcgagg aggaggcaga cgcccgtccg
     2701 gaagacgatg ccgggaaggt acgtcgggtt gaggaaaccg tcccagaagc cgcgcgtctc
     2761 gagccaccga cccggcgtca gcatgaatcc gaggattccc tggatgacca cgagcgacat
     2821 gtaggccgcg aacgcgtaga gaacgatgag gaggcggtgc gtgcgggggc ggacgcggtc
     2881 ccacgtcgcg taccacgcga gggccgtgac gacctcgagc aggaagaacg cccactcgat
     2941 cgcccatccc cacacgaacg tgtgaatgag tgccgacgtc gcggcggggt ggacgaggcc
     3001 gatcacgaac cagatggcga cgcccgagat cgcgccgaag acggtcgaga cgaggatcag
     3061 catcagcgac gcgcgcttca ggagcgcgcg aagcgccgga tcgggacggc ggccgaggga
     3121 cgtttccgcc cacgccatca cgaggccgcc gccgacggcg aagtgagaca cgacgacgtg
     3181 aaggatcgcg atcgctccga tcaggacgcc tccgccgacc gccagatccc agatcgggta
     3241 gtccatggga aagacctccg cccggattct gacgaatccc cgggaacggc cgccgtcctg
     3301 agacggcgtc gctcagacct cgttgagatt ctccacggtc ccgcgctccc acgcgcccct
     3361 ggccgcgagg gcgaccgagt ctcgtctgcg aaaatctttg aaaccgagtc tcgatgatcg
     3421 tagattacgg gcctcggggg gaatcgaatg tctcaggaag ctcctctgca ccgccgcgtc
     3481 cggcgggggg cgtcccgcac ggcgcggagc ctcgcggtcc tggtcccgtt cctcgcgtcc
     3541 gcggccgccc tcgcggggcc ctccgggccc tacgcgccgc cccgcgacgt gtccacggaa
     3601 ggccaccggg tcacgtggct cttctggtac acgacgggcc tcgggatcgt cgccttcacg
     3661 ctcgtctgcg cgagcctcgt gtacatcgtc gcgcgttacc gcgcgcgggc cgggcaccgc
     3721 gccgtgtacg acaagggaac ggaccgccgg agcctcgtcg tcacgggatc gctcgcgctt
     3781 ctcgtgtttc tctcgatcga cctcgtgctc gtccaccgtt cacgcgcgga catcgaggaa
     3841 tacctgttca agtggccgac gtcggcggac accgtccgga tcgaggtcat gccgcagcag
     3901 tgggtgtgga acttccggta cgcgggcgcc gacggcgtct tcaacacggc cgacgacgtc
     3961 gtgacgctga acgagatgtg gatcccgaag ggccgcccgg tcttcctgaa cctgaaggcc
     4021 aaggacgtca tccacagctt ctacgtcccg aacttccgca tcaagcagga cgcgaacccg
     4081 ggccacgtga cccggatgtg gttccaggcg aaggagacgg gctcgatgga gatcgcctgc
     4141 tcgcagctct gcggctgggc ccactacaag atgaaggccg acctcacggt gatggagaac
     4201 gacgcgttcg agcggtggta cgccgaggcg cagggcgacg cgaagcgccg gttcgacccg
     4261 gccgacaccg aggcgcagtg ggggtgggaa tgggtggcga agtgacgacg cggcgcccga
     4321 ggctcgcgta cgccggtccg gactttcacg ccgagcagca catccacccc gaccccgact
     4381 cgttcctgag gcggtgggtc ttctcgctcg accacaaggt catcgcgaag cagttcctct
     4441 ggctgggact cgcgttcctc gcgatcggcg ggacgatggc gatgatgatc cgctggcagc
     4501 tcggccaccc ggggaccgcg ttccccgtcc tcggcgagct catctggccc cagagcggcg
     4561 gcgtcatgcc cccgcagtcc tattcgtcgt tcttcacgat gcacgggacc atcatgatct
     4621 tcttcgcgat cacgccgatc ctgatcggcg cgttcggcaa cttctgcatc ccgctcatga
     4681 tcggcgcgcg ggacatggcg ttcccgacgc tgaacatgct ctcgttctgg acgatggcgc
     4741 tctcgagcgg catcctcgtc gcgtcgttct tcgttccggg cggctccgcg tcgggcggct
     4801 gggtgtccta tccgacgctc tcgacgctcg tcggacagcc ggcgccgggc cagacgctct
     4861 ggatcgtcgc gatcttcctc gcgggctcgt cgtcgatcat gggcgccgtc aactacatca
     4921 cgaccgtcat ccgcctccgc gcgcccggca tgggctactt cgacatgccg ctcaccgtgt
     4981 ggggcctgtg gctcacggcg atcctgaacg cgctcttcgt ccccgtgatc gcggcgggaa
     5041 tgctcctcct cttcctcgac cgggtcttcg ggagccattt cttcgtggcc ggcatggccg
     5101 tgacgaaggg cggcggcgac ccgatcctct accagcacct cttctggatc ttcgggcacc
     5161 ccgaggtcta catcctcatc ctccccgcgt ggggcgtcgt gtccgacgtg ctctcgttct
     5221 tctcgcgcaa gcccgcgttc ggctacaagg cgacggccct ctcgatgacc gccatcaccg
     5281 tgctctcgat ggtcgtctac ggccaccaca tgttcgtcgt cggcatgagc ccgctcctct
     5341 cggagtcgtt catgacgctc acgatgctca tctcgatgcc gtcggcggtc ttcttcctga
     5401 actggctcgg gacgatgtgg cgcggctcga tccgcttcac gacgccgatg ctcttctcga
     5461 tgggcgtcgt cttcgtgttc ggcctcggcg gcctcacggg cctgcacctc ggcgcgatcg
     5521 gcacggacgt gtacctacac gactcgtact tcgtcgtcgg ccacttccac ctcacgatgg
     5581 cggcgtccgt gctcctcggc gccttctcgg cgatctactt ctggttcccg aagctcttcg
     5641 gcaagatgat gaacgagagg ctcgggaagc tgcacttctg gctcacgatc gtcccgatcg
     5701 tcctcgtctt cggcgcccag ctcgtcatcg ggtacgcggg gatgcagcgg cgcctctaca
     5761 acctcgattc ctacacgttc ctccagcacc tgcagccgct gaacctcttc atcacccgcg
     5821 cggcgctctt cctcggcgcc acgcagttcc tcttcgtcgc gaacttcctc tcgagcctga
     5881 tctcgggcgc gaaggcctcc gagaacccct gggaggtcgg cacgctcgag tggacggtcc
     5941 cctcgccgcc cccgcatcac aacttcgacg agatcccgct cgtcctccac gggccgcacg
     6001 agttcagcca ccccggcgtc acggggaagg actggctcgc gcagaacgag ccgctcgagg
     6061 ccgagcccgt gccgctgggg gccgtgggct cgtgaacgcg gccgctctca cgcgggtcgc
     6121 cgcgcgcgag gagtcccggc ggacgtcgct cgtcgggatg gtcatggcgc tcgggtcgtg
     6181 gacgatgctc ttcgtgagcc tcttcttctc gtacgcggtc ctgcgcctcc gcgcaggcga
     6241 gtggccgccg gacggcctgg agccgcttcc gcgcctcctt ccgttcctga acacgctcgt
     6301 gctcgcggcc tcgagcctcg tgctccacgc cggcgtccgt tccgcggcgg cggtgcggcc
     6361 gcgcgcgctc ctctcggccc tgcgccggac gctcgcgctc gggtcgctct tcctcgcgct
     6421 tcagctcgcc gtctggatcc cgctctggcg ctcgggcttc cggatggatt ccggcgtctt
     6481 cgggtcgatc ttctacggcc tcaccgtgtt ccacgcgctc cacgtcctcg cgggcctcgt
     6541 cgcgctgctc gtcctcgtgc cgggcgccgc gaagggccgc tgggtctcgg gggcgcagag
     6601 ccccgtgcgc ctcacggcga tgttctggca cttcgtggac gccgtctggg tcctcatgtt
     6661 cgtgacgatc tacctgctct gagagaagaa agaaaagaga agagaagatt gctatgaagg
     6721 ttggaacgcg ggtcgcggcg ggcggggtcg gtgcgtttct cggcgcgctc cttctgctcg
     6781 gttcgggatg ctcgggtccg ggcgcccgtt tctcgaagtc ccagacgctc ggcggcaagc
     6841 gcgtctccgc ccgccgcctc aaccacggcc gcgagctctt cgtgacgtac tgcgccgcct
     6901 gccacggcct cgagggcgac ggcaaggggc cggccgccgt cggcctccgc ccgccgccgc
     6961 gcaacttcaa gcagggtcag ttcaagttcg cggccgtcgc gagcggccag ctcccgaacg
     7021 acgaggacct gctgcggatc gtccagaagg ggctgcacgg cagcgcgatg ctcccgtgga
     7081 acgacgtccc cgaacgcgac ctcctcgaca tcatccagta cctgaagacg ctctcgccga
     7141 agtgggccga gaagacggcc ggcgagccga tcgtcccgtc cccggacccg tacggcgcgg
     7201 cccgcaaggc ggaagccgtc acgcgcggga tgaaggtcta ccacggcctc gcgcagtgcc
     7261 tctcctgtca cccggcctac gagacgccgg agacgatcca ggccgcgagc ctcgagctca
     7321 gcaagcgcga gaccacgctg cggccggacc cgtaccacgc ctccccgaag gagagcgact
     7381 acggcgtcaa gatcatgccg ccggacttca cgcgagacca cgtccgctcg ggcgaggcgc
     7441 tcacggacat ctaccgcacc gtcgcctcgg gcatcggcgg cacggcgatg ccgacgtgga
     7501 agggcgccct cccggaggag gacatctggg cgatggcgta ctacgtgcgg tcgctcatcg
     7561 acatcaagaa cacccccgaa gccgacgcgc gccgcgcaaa gctcgtcgcg atggcgcctc
     7621 ccgagacgac gacggcagcg aagtaagaga agaagagaag agaagatttt cttagaaggt
     7681 aatagaggct gatggcagca cctgttttcg cgagtggagg ttccgcagcc gcacgcgtgg
     7741 ttggcggagc cgcgccctcc ccttcacctt ctaagaaatc cccctcttcc tttcttcttc
     7801 cccaacgcct cgcggcccgg cttacgggca gcccgtggtt ctgggccgtt ttcgtcttcg
     7861 cggtcttcgc cgtcccggtc ttccgctccg tgacgcggcc gcttccggcc gcgccgcccg
     7921 tgctcggcga gctcccggcc ttcgcgctca ccgaccagca gg
//
LOCUS       DAOMFY010000178         7935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2850_length_7935_cov_10.043909, whole genome shotgun sequence.
ACCESSION   DAOMFY010000178 DAOMFY010000000
VERSION     DAOMFY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2850_length_7935_cov_10.043909"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLB01_13375"
     CDS             complement(<1..547)
                     /locus_tag="PLB01_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78339.1"
                     /translation="MSPDGPAPSPSDLAPQDVVLRFLESTGRPSEARLYLELFRARPR
                     EQFAALAIDANVMAGAADAVVQDLRFLAALDLFPTVVLGIFQPADAAAHARVLTRHLE
                     AEGVAVETLSASEPGLARRLTAAAAAGRVPAVVFEPAAVSRQRYVVTPRQHLRQWRQT
                     GRADVAFARKHPARAADLFAAH"
     gene            complement(587..1243)
                     /locus_tag="PLB01_13380"
     CDS             complement(587..1243)
                     /locus_tag="PLB01_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HQQ78340.1"
                     /translation="MSANADAPLLRESAAWRLLGLLFERPREGWRDEVDALEPIAADP
                     TLSLAVAAAREEACEEAFLAILAPGVVSPREVTWQRGADGGQLLAEIRETYDAFAFRP
                     VTEEPPDHVSVEAGFVGWLLLKEAYARAAELPDAAALTAEARARFQKAHVARLAASLA
                     ENLDRWGVSYLTLAAAALAARAGEPEAMPSLDAPPTPCPEGCTFAGDEEDEEGEVLDA
                     "
     gene            complement(1240..2013)
                     /locus_tag="PLB01_13385"
     CDS             complement(1240..2013)
                     /locus_tag="PLB01_13385"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HQQ78341.1"
                     /translation="MKAVRAAALSASPSDAAWAALPVHVAPLVPQDMVEPRLLKATTP
                     EVRVRAMHDGTSVAFLLEWADATKNDVPGPARFTDACAVQLPAAPPARDLPAPQMGEK
                     GRGVEITYWRASWQAVVDGRGDTLRDLYPNSTVDHYPFEAKSLADGSPEKKEAALRYA
                     PARALGNAMAGPRAKPVEDLVAEGPGTLAPGPAAGSNGKGVRTAAGWSVVLSRRLPNG
                     VTAPARSQVAFAVWEGADEESGARKMRTVWIPLNLEGKP"
     gene            complement(2156..3280)
                     /locus_tag="PLB01_13390"
     CDS             complement(2156..3280)
                     /locus_tag="PLB01_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HQQ78342.1"
                     /translation="MARVKNWQIQREMSYWYPEARPKKQFAAIFDVNKCIACQTCTLA
                     CKTTWTSGKGQEYMLWNNVESKPYGYYPLAWDVQILEKLGPQTWDGARYEGQTIFEAA
                     PPGKRVLGWRPEEIDYAHPNVGEDDCSGRIEKGAGFSGPHMAWFYYLARICNHCTYPA
                     CLASCPRGSIYKRPEDGIVLVDQGRCRGYQECVQACPYKKVFFNPMTGTSEKCIACFP
                     KIEAGNQPQCFVNCIGKIRMAGFINTPEKADPSNPIDYLVHVRKVALPLFPQLGLEPN
                     VYYIPPVHGAEPYLEQMFGPGAHKAIETYRTADQDLAGLLALFGCSEEVMPRWKRAGD
                     TIIGLKEDGKTEIVRVPYREPAHVRAAVDLRHGVQRTNCP"
     gene            complement(3284..6736)
                     /locus_tag="PLB01_13395"
     CDS             complement(3284..6736)
                     /locus_tag="PLB01_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQQ78343.1"
                     /translation="MNNDITRRRFLEAAAASGFGALVASAGKAWGLDAIANPLAAYPD
                     RGWEKVYRDLFKPDSSFTFLCAPNDTHNCLLRGFVRSGVLTRIGPTMRYGEATDLDGN
                     KASHRWDPRVCQKGLALTRRFYGDRRLSHCKVRTGYKAWIEKGFPRGADGKPPAEYFN
                     RGRDTWVRVTHEEAAALVAKCLKNIAETYAGEEGARKLRAQHYDEKMIEATGGAGTQV
                     LKFRGGMPLLGVTRVFGLYRMANSMALLDAQIRKLGPDKAIGGRGFDNYSWHTDLPPG
                     HPMVTGQQTVEFDLHAVEHAKTLVVWGMNWITTKMPDAHWLTEARVKGTRVVVIACEY
                     SATSTKADDVLVVRPGTMPALGIGLAHVIMKEKLYDADYVRRFTDLPLLVRMDSLTYL
                     KAQDVFGGTAAALSNQTHVMAEGEKEPPAGQHRDQLIPGKLRDAWGDYVWWDQAAKAP
                     RAVTRDDVGAKSKIADPLLQGAVEVGLKDGTKVRCRPVYDLVYEYAAHWDPKTVEEAT
                     WAPAAAVESLARQLAKSPGTTLFAVGMGPNQFFNNDNKDRALFLLASLTGNVGKLAGN
                     VGSYAGNYRVAIFNGAPQYINENPFDLELDPAKPARAKQYWKAESAHYYNHEDHYLKV
                     GKKTLTGSTHIPCPTKSLWFANANSILGNVKWHYNTVVNVLPKIEMIAVNEWWWSTSC
                     EWADVVFAVDSWAELKYPDMTASVTNPFLNVFPKTPLPRIFETRGDIEVLALVGDAMA
                     KETGDKRFSDTWKFVKEKKVEVYLQRVLDASANTRGYKFEEIEAKAKEGIPAIVMSRT
                     NPKCVGYEQVTDSRPWYTKSGRLEFYREEPEFIEAGENLPVHREPIDSTFYEPNVIVA
                     PKHEAIRPVGPEAYGVAKDDLSCEVRQGRNVVMTWEETKKTAHPLAKQGYKFIFHTPK
                     YRHGSHTLPIDTDMVAMLFGPFGDVHRHDKRTPFVTEGYVDIHPSDAKELGVEDGDYV
                     WVDSDPEDRPFKGWQKNPKDYAFARLLCRARYYPGTPRGVTRMWFNMYGATPGSKEGH
                     ETRPDGLAKNPRTNYQALFRSGSHQSATRGWLKPTLMTDSLVRKGLFGQGIGKGFLPD
                     VHCPTGAPREAIVKITKAEPGGLEGKGLWRPAEKGFRPRYENASMQKYLAGGFVSKND
                     GKRG"
     gene            complement(6733..>7935)
                     /locus_tag="PLB01_13400"
     CDS             complement(6733..>7935)
                     /locus_tag="PLB01_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQQ78344.1"
                     /translation="AGAAVFERHDCLACHKLDGRGGTLRPDGGGMEGPDLSAAGARPV
                     ASGWYESHLDKSAKAAGPPWRGSVAALSAADRAALATFLETRVGAPGLTEAKALFHSL
                     GCRGCHKVNGVGGADGPDLTREGRLDPGRLDFTHVPGGRSVPAWLAEHFRAPATLVAN
                     SQMPALGLTETEIDRLVFYLLSLRTESSAANTQPPDRLRVERFGAREFATDGATLYGA
                     FCSACHGPAGEGMRYAGMAAFPAIGNSDFLALASDEFLAATIDKGRPGRRMPGWADPA
                     SGLRPEERKAVVAYVRSFAGGIAPGAPTEPRRFVAPSLKGGDTVYARACAGCHGAKGQ
                     GAEGPALANPVLLESASDRYLVETIRRGRRGTSMEGFATPSSTRASLTDAEIESVVAL
                     LRTWEKTR"
BASE COUNT         1059 a         2724 c         2876 g         1276 t
ORIGIN      
        1 cgtgggcggc gaacaggtcc gcggcgcggg ccgggtgctt ccgggcgaat gcgacgtccg
       61 cccgcccggt ctgccgccac tgtcgcaggt gctgtcgcgg ggtcaccaca tagcgctgcc
      121 ggctcaccgc ggccggctcg aagacgaccg ccggcacgcg ccccgccgca gccgccgccg
      181 tgaggcggcg ggcgagcccg ggctccgacg cggacagggt ctcgacggcg accccctcgg
      241 cctcgaggtg gcgggtgagg acgcgggcgt gcgccgccgc gtcggcgggc tggaagatcc
      301 cgaggacgac ggtcgggaac aggtcgagcg cggcgaggaa gcgcaggtcc tgaacgacgg
      361 cgtcggccgc gccggccatg acgttcgcgt cgatcgcgag cgccgcgaac tgctcgcgcg
      421 ggcgcgcgcg gaagagctcg aggtagagcc gcgcctcgga cgggcgcccc gtgctctcga
      481 ggaagcggag gacgacgtcc tgcggggcga gatccgaggg cgaaggcgcc gggccgtcgg
      541 ggctcatgtc cccgggagga tacggaactt ccctcggatc atcggatcag gcgtcgagga
      601 cctcgccctc ctcgtcctct tcgtctcccg cgaacgtgca gccctcgggg cagggggtcg
      661 ggggcgcgtc gagggacggc atcgcctcgg gctcgcccgc ccgcgccgcg agcgcggcgg
      721 cagcgagagt gagataggaa acgccccagc ggtcgaggtt ctccgcgagg gacgccgcga
      781 gccgcgcgac gtgcgctttc tggaagcgcg cccgcgcctc ggcggtcagc gccgcggcgt
      841 cgggaagctc cgcggcccgc gcgtaggcct ccttcaggag aagccagccg acgaagccgg
      901 cctcgacgga gacgtggtcg gggggctcct cggtcacggg ccggaacgcg aacgcgtcgt
      961 acgtctcgcg gatctcggcg aggagctggc ctccgtctgc gccccgttgc cacgtgacct
     1021 cgcgcggcga gacgacgccg ggcgcgagga tcgcgaggaa cgcctcctcg cacgcctcct
     1081 cccgcgccgc ggcgacggcg agggagagcg ttggatcggc cgcgatgggc tcgagcgcat
     1141 cgacctcgtc gcgccagccc tcgcgcgggc gctcgaagag gaggccgaga agcctccagg
     1201 ccgcggactc gcggaggagc ggggcgtcgg cgttcgcgct catggctttc cctcaaggtt
     1261 gagcgggatc cacacggtgc gcatcttccg ggcgcccgac tcctcgtccg cgccctccca
     1321 cacggcgaac gcgacctgcg accgggccgg tgcggtcacg ccgttgggca gccgccgcga
     1381 cagaacgacg gaccagcccg cggcggtgcg cacgcccttg ccgttggaac cggctgccgg
     1441 gccgggtgcg agcgtgccgg gaccctcggc gacgagatcc tcgacgggct tcgcgcgcgg
     1501 gcccgccatg gcgttgccga gcgcgcgcgc cggcgcgtag cgcagcgcgg cctccttctt
     1561 ctcgggcgag ccgtccgcga ggctcttcgc ctcgaacggg tagtggtcga ccgtcgaatt
     1621 cggatagagg tcgcgcagcg tgtcgccgcg cccgtccacg acggcctgcc aggacgcgcg
     1681 ccagtaggtg atctcgacgc cgcgcccctt ctcgcccatc tgcggggcgg gcagatcgcg
     1741 ggcgggcggc gcggcgggga gctggaccgc gcacgcgtcc gtgaaacgcg cggggccggg
     1801 cacgtcgttc ttcgtcgcgt ccgcccactc gaggaggaac gcgacgctcg tgccatcgtg
     1861 catcgcgcgg acgcggacct ccggcgtcgt cgccttgagg agccgcggct cgaccatgtc
     1921 ctgcgggacg agcggcgcga cgtgcacggg gagcgcggcc catgcggcat cggagggcga
     1981 cgcagaaaga gcggccgcgc gaaccgcctt cacttcgccg ggagagagat tcggcttctt
     2041 cgcgcacgcc gccgtatcga cgaccaccaa cgccgccgcg agaagagcgc cattccgaag
     2101 tgccctattc accttcatag aaatctcccg gtctttcttt tcttcttccc atcgctcacg
     2161 ggcagttcgt gcgctggacg ccgtggcgga ggtcgacggc ggccctcacg tgcgcgggct
     2221 cgcgatacgg gacgcggacg atttccgtct tgccgtcctc cttgaggccg atgatcgtgt
     2281 cgccggcgcg cttccagcgc ggcatgacct cctcgctgca gccgaagagc gcgagcaggc
     2341 ccgcgaggtc ctggtccgcc gtcctgtacg tctcgatagc cttgtgcgcg ccgggcccga
     2401 acatctgttc gaggtacggc tcggcgccgt ggacgggcgg gatgtagtag acgttcggct
     2461 cgagtccgag ctgcgggaag agcgggagcg cgaccttccg aacgtggacg aggtagtcga
     2521 tcgggttcga cgggtctgcc ttctcgggcg tgttgatgaa ccccgccatc cggatcttcc
     2581 cgatgcagtt cacgaagcac tggggctggt tgcccgcctc gatcttcggg aagcaggcga
     2641 tgcacttctc ggacgtgccc gtcatcgggt tgaagaagac cttcttgtac gggcaggcct
     2701 gcacgcattc ctggtagccg cggcagcgcc cctggtcgac gaggacgatc ccgtcctcgg
     2761 ggcgcttgta gatcgagccg cgcgggcagg acgcgaggca cgccgggtac gtgcagtggt
     2821 tgcagatgcg cgcgaggtag tagaaccacg ccatgtgcgg gcccgagaag cccgcgccct
     2881 tctcgatcct gccggagcag tcgtcctcgc cgacgttcgg gtgcgcgtag tcgatctcct
     2941 cgggccgcca gccgaggacg cgcttgcccg ggggggccgc ctcgaagatc gtctggccct
     3001 cgtagcgggc gccgtcccag gtctgcgggc cgagcttctc gaggatctgg acgtcccacg
     3061 cgagcgggta gtagccgtag ggtttggact cgacgttgtt ccagagcatg tactcctggc
     3121 ccttgccgga cgtccacgtc gtcttgcagg ccagcgtgca cgtctggcac gcgatgcact
     3181 tgttcacgtc gaagatcgcc gcgaactgct tcttcgggcg ggcctcgggg taccagtagg
     3241 acatctcccg ctggatctgc cagttcttca cgcgcgccat ggcttacccc ctcttcccgt
     3301 cgttcttgga cacgaacccg cccgcgaggt acttctgcat cgaggcgttc tcgtagcgcg
     3361 gcctgaagcc cttctcggcg gggcgccaga ggcctttgcc ctcgaggcct ccgggctcgg
     3421 ccttcgtgat cttcacgatc gcctcgcgcg gcgccccggt cgggcagtgg acgtcgggca
     3481 ggaagccctt gccgattccc tgcccgaaga gaccctttcg cacgagcgaa tccgtcatga
     3541 gcgtcggctt gagccagccg cgcgtcgcgg actggtgcga gcccgagcgg aagagcgcct
     3601 ggtagttcgt gcgcgggttc ttcgcgaggc cgtcgggccg cgtctcgtgg ccttccttcg
     3661 agcccggcgt cgcgccgtac atgttgaacc acatgcgcgt cacgccgcgg ggcgttcccg
     3721 ggtagtaccg cgcgcggcag aggaggcgtg cgaaggcgta gtccttcggg ttcttctgcc
     3781 agcccttgaa cggccggtcc tcggggtcgg agtcgaccca cacgtagtcg ccgtcctcga
     3841 cgccgagctc cttcgcgtcg gaggggtgga tgtcgacgta gccctccgtc acgaacggcg
     3901 tccgcttgtc gtggcggtgg acgtcgccga acggcccgaa cagcatcgcg accatgtccg
     3961 tgtcgatcgg gagcgtgtgc gagccgtgcc ggtacttcgg cgtgtggaag atgaacttgt
     4021 aaccctgctt tgcgagcggg tgcgccgtct tcttcgtctc ctcccacgtc atcacgacgt
     4081 tgcggccctg ccgcacctcg cacgagaggt cgtccttcgc gacgccgtac gcttcgggcc
     4141 cgacgggccg gatcgcctcg tgcttcggcg ccacgatgac gttcggctcg tagaacgtcg
     4201 agtcgatggg ctcgcggtgg acgggcaggt tctcgccggc ctcgatgaac tcgggctcct
     4261 cgcggtagaa ctcgaggcgc ccggacttcg tgtaccacgg ccgggagtcc gtgacctgct
     4321 cgtacccgac gcacttcggg ttcgtgcggc tcatgacgat cgccgggatt ccttccttcg
     4381 ccttcgcctc gatctcctcg aacttgtagc ccctcgtgtt cgcggaggcg tcgaggaccc
     4441 gctgaaggta gacctcgacc ttcttctcct tcacgaactt ccacgtgtcg gaaaagcgct
     4501 tgtcccccgt ctccttcgcc atcgcgtcgc cgacgagggc gaggacctcg atgtcgccgc
     4561 gcgtctcgaa gatgcgcggg aggggcgtct tcgggaagac gttcaggaac gggttcgtga
     4621 ccgaggccgt catgtcgggg tacttcagct cggcccacga gtccaccgcg aagacgacgt
     4681 cggcccactc gcaggacgtc gaccaccacc actcgttcac cgcgatcatc tcgatcttcg
     4741 ggaggacgtt gacgaccgtg ttgtagtgcc acttcacgtt cccgaggatc gagttcgcgt
     4801 tcgcgaacca gagcgacttc gtcgggcagg ggatgtgcgt cgagcccgtg agcgtcttct
     4861 tcccgacctt caggtagtgg tcctcgtggt tgtagtagtg cgccgactcc gccttccagt
     4921 actgcttcgc gcgcgcgggc tttgcggggt cgagctcgag gtcgaagggg ttctcgttga
     4981 tgtactgcgg cgccccgttg aagatcgcga cgcggtaatt gcccgcgtac gagccgacgt
     5041 tccccgcgag cttcccgacg ttgcccgtga gggacgcgag gaggaagagc gcgcgatcct
     5101 tgttgtcgtt gttgaagaac tggttcgggc ccatgccgac cgcgaagagc gtcgtgcccg
     5161 ggctcttcgc gagctggcgc gcgaggcttt cgaccgcggc cgccggggcc cacgtggcct
     5221 cctcgacggt cttcgggtcc cagtgcgcgg cgtactcgta gacgaggtcg tagacgggcc
     5281 ggcagcggac cttcgtcccg tccttgagcc cgacctcgac cgcaccctgg agcagcgggt
     5341 cggcgatctt cgacttggca cccacgtcgt cgcgggtgac ggcccgcggc gcctttgcgg
     5401 cctggtccca ccacacgtag tcgccccagg cgtcgcggag cttcccgggg atgagctggt
     5461 cgcggtgctg gccggccggc ggctccttct cgccctcggc catgacgtgc gtctggttcg
     5521 agagggcggc cgcggtcccg ccgaagacgt cctgcgcctt gaggtacgtg agcgagtcca
     5581 tccggacgag gagcgggaga tccgtgaagc gccgcacgta gtcggcgtcg tacagcttct
     5641 ccttcatgat gacgtgggcg aggccgatcc cgagggcggg catcgtgccg ggcctcacga
     5701 cgaggacgtc gtcggccttc gtcgaggtcg ccgagtattc gcacgcgatc acgacgacgc
     5761 gcgtcccctt gacgcgcgcc tcggtgagcc agtgcgcgtc cggcatcttc gtcgtgatcc
     5821 agttcatgcc ccagacgacg agcgtcttcg cgtgctcgac tgcgtggagg tcgaactcga
     5881 ccgtctgctg gcccgtgacc atcgggtggc cgggcggaag gtccgtgtgc cacgagtagt
     5941 tgtcgaaacc gcggccgccg atcgccttgt cgggcccgag cttccggatc tgggcgtcga
     6001 ggagcgccat cgagttcgcc atccggtaga ggccgaagac gcgcgtgacg ccgaggagcg
     6061 gcatcccgcc gcggaacttc aggacctgcg tcccggcgcc gcccgtcgcc tcgatcatct
     6121 tctcgtcgta gtgctgggcc cggagcttcc tggccccttc ctcgcccgcg tacgtctcgg
     6181 cgatgttctt gaggcacttc gcgacgagcg cggcggcctc ctcgtgcgtg acgcgcaccc
     6241 acgtgtcgcg cccgcggttg aagtactcgg cgggaggctt cccgtccgcg ccgcgcggaa
     6301 agcccttctc gatccacgcc ttgtagcccg tccgcacctt gcagtggctg agccgcctgt
     6361 cgccgtagaa gcggcgcgtc agcgcgaggc ccttctggca gacgcgcggg tcccagcggt
     6421 ggctcgcctt gtttccgtcg aggtccgtcg cctcgccgta gcgcatcgtc ggcccgatgc
     6481 gcgtgaggac gccggagcgc acgaagccgc gcaggaggca gttgtgcgtg tcgttcggcg
     6541 cgcagaggaa cgtgaaggag gagtccggct tgaagaggtc gcggtagacc ttctcccatc
     6601 cgcggtcggg gtaggccgcg agcgggttcg cgatcgcgtc gaggccccac gcttttccgg
     6661 cggaggcgac gagcgcgccg aagcccgagg cggcggcggc ctcgaggaag cgccggcggg
     6721 tgatgtcgtt gttcatcgcg tcttctccca cgttcggagc agggccacga ccgattcgat
     6781 ctcggcgtcc gtgagggagg cccgcgtcga ggagggcgtg gcgaacccct ccatcgacgt
     6841 gccgcggcgc ccgcggcgga tcgtttcgac gaggtagcgg tcggaggcgg attcgagcag
     6901 gaccggattg gcgagggcgg gaccttccgc cccctggccc ttcgcgccgt ggcagccggc
     6961 gcatgcgcgc gcgtagaccg tgtcgccgcc cttgagagag ggcgcgacga agcgccgcgg
     7021 ctcggtcggc gcgccgggcg cgatgccgcc ggcgaaggag cggacgtacg cgacgacggc
     7081 cttgcgctcc tcgggccgga ggcccgaggc gggatcggcc cagcccggca tcctccggcc
     7141 ggggcggccc ttgtcgatcg tcgcggcgag gaactcgtcc gaggcgagcg cgaggaagtc
     7201 gctgttgccg atggcgggga aggcggccat cccggcgtag cgcatgcctt cgcccgcggg
     7261 cccgtggcac gccgagcaga acgcaccgta gagcgtcgcg ccgtccgtcg cgaactcgcg
     7321 cgcgccgaag cgctcgacgc ggagacggtc ggggggctgc gtgttcgcgg ccgagctctc
     7381 ggtgcgcagc gagaggagat agaagacgag gcggtcgatc tcggtctcgg tcagcccgag
     7441 cgccggcatc tgcgagttcg caacgagcgt cgcgggcgcg cggaagtgct ccgcgagcca
     7501 tgcaggaacg ctccggccgc ccggcacgtg cgtgaagtcg agccggccgg ggtcgaggcg
     7561 cccctcgcgc gtgaggtccg gcccgtcggc gccaccgacg ccgttcacct tgtgacagcc
     7621 gcggcagccg agcgaatgga agagggcctt cgcctccgtg aggccgggag cgccgacgcg
     7681 cgtttcgagg aacgtcgcga gcgccgcgcg gtccgcggcg gagagcgccg caacggagcc
     7741 gcgccacggg ggtccggccg ccttcgcgct cttgtcgagg tgcgactcgt accacccgga
     7801 ggcgacgggc cgggcgccgg cggccgagag atccggaccc tccatgccgc cgccgtcggg
     7861 ccgcagcgtt ccgccgcggc cgtcgagctt gtggcaggcg aggcagtcgt gccgctcgaa
     7921 gacggcggcg ccggc
//
LOCUS       DAOMFY010000179         7932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2853_length_7932_cov_9.389869, whole genome shotgun sequence.
ACCESSION   DAOMFY010000179 DAOMFY010000000
VERSION     DAOMFY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2853_length_7932_cov_9.389869"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLB01_13405"
     CDS             complement(<1..1101)
                     /locus_tag="PLB01_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78345.1"
                     /translation="MRRAFSAFVAVETFVFLAREDRWAVLFFFTRETWLLVTLLWLKN
                     VALALLAGVLFAALVRATERLERATDLRAMRATRGVVAIALLAAGIGIASRFAFPEWI
                     PPGLWFDPPFEARALLERPDGLPWIGGAPLHADPAAAGNRELVSFVYLHFYDLVFRVF
                     GRGEAGFGALSAVPGVLAIPAAAWLAWEVSGGLAASLATVAVALGLWPLVFSRWGYTA
                     AALIPLALVAGAATLAALRTRRVAFALLAGAALGLSLHTHSSSWAVAAGLGVFSLSLL
                     RARENRALVLAAWLTAGVAFLPFGIGYLQNPANLGGRIRDVPAGSRVSGAYGAPVTGP
                     LRVPATLLTNALDYAGVLVWTPDPNARDGLPER"
     gene            complement(1103..4024)
                     /locus_tag="PLB01_13410"
     CDS             complement(1103..4024)
                     /locus_tag="PLB01_13410"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HQQ78346.1"
                     /translation="MNARETKAGTRTEKANGAAPRSGARGLTFRRVYTDGVTHPYDAI
                     EWELRTAAITNEKGEVFFEQKDVEVPKAWSMTATNIVAQKYFSGKPGTPQRERSVRQL
                     IGRVVETITGFGAKGGYFRTAADRDAFSDELTHILVNQAASFNSPVWFNVGVEPKPQA
                     SACFINSVGDSMSSILDLAKTEGMLFKFGSGTGSNLSSLRSSTESLSSGGIASGPVSF
                     MKGFDSFAGAIKSGGKTRRAAKMVILNVDHPDIEEFILCKEKEEKKAWDLIDAGWDGS
                     FNGEVYSNIAFQNANHSVRVTDEFMKAVESGGTHTTKAVTTGEPMGTFDAKELLVKMA
                     ESAWVCGDPGMQYDTLINKWNPCKATHRINASNPCSEYMFIDDSACNLASLNLMKFAR
                     EDGGFEIEKFQHAVDVVFAAQEMLVSEASYPTPAIEKNSHDFRPIGLGYANLGALLMY
                     NGLPYDSDEGRHYAAAVTSLMHGQAYLTSSKIASEMGPFAGYPPNRDAFLEVISMHRD
                     AAYEVARPGVPKDLWEAQRAVWDLTLESGRQHGYRNAQATVLAPTGTIGFMMDCDTTG
                     VEPDLALVKYKKLVGGGTIKIVNQTVPHALTRLGYSDAEMHAIVRHIDEKGTIEGAPG
                     LKSEHLPVFDCAFKALGGTRSIAPMGHVRMMSAVQPFLSGAISKTVNMPNDATVEEIA
                     DVYMQGWKLGLKAIAIYRDGCKRTQPLNTSASKNDDGVKGTRDARGNTIAAAAPVAIA
                     PALRRHKLPDERRAITHKFSVAGHEGYVTVGLYEDGMPGEIFLTMAKEGSTISGLMDA
                     FATAISLTLQYGVPLEALVEKFSHMRFEPAGYTKNPEIPIAKSLVDYIFRWLASKFLT
                     ADLKEKVGIISREEPGPATSASGYKATASVTTLAPVAEAAMAPITAAFQAIAGIRSET
                     PAEAAEPRAIGFAVPLAPASKLTFENSADAPSCHECGSLMVRGGACYKCLNCGATSGC
                     S"
     gene            4368..4739
                     /gene="erpA"
                     /locus_tag="PLB01_13415"
     CDS             4368..4739
                     /gene="erpA"
                     /locus_tag="PLB01_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HQQ78347.1"
                     /translation="MSSAPETATQVASPSVFLLSVTDRAVAKVKEFAAKMPEAANKPL
                     RVFIQGGGCSGFQYGFTFDDKRPTDHVLAVKDIEVVVDPASALKLHGSTVDFVEDMRG
                     AGFAVENPNATSGCGCGKSFS"
     gene            4693..6015
                     /locus_tag="PLB01_13420"
     CDS             4693..6015
                     /locus_tag="PLB01_13420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ78348.1"
                     /translation="MRTRRPAAAAENPSADDRERETRHAGFPHPSARTLRVLRRLVKN
                     PSILKIVFLTVFIDLLGFGIVIPLLPLYAEKYHPSPLVFGLLMSSYSAMQFLFGPLLG
                     RLSDRYGRRPVIIFSLAGTVVGYVLFAFARSLPMLFVARLLPGATGGNIGTAQAVIAD
                     STAPTERARGMAMVGIAFGLGFIFGPAIGGFAVHLGEAAPGLFAAGLSLVACVWAFFK
                     LPETRPAGKVAPSGPLFSAGPLLAAFRRPEIGALMLLSFVTTTAFANFESTFAQFLSL
                     RLGAGPSTVAWFFVFVGVCSVVVQGGLVRRLAPRVGEARLVMAGCVFLIAGFLLLRLG
                     TTPPRLLAAIAVIALGIGITTPTLSSLVSRRTAATEQGEVLGAYQSMASLGRVFGPFA
                     GENLYLRVGPDAPHWAAAALQGGALVLSAAGLLRENAAAPSRAEESPR"
     gene            6012..6566
                     /gene="sufT"
                     /locus_tag="PLB01_13425"
     CDS             6012..6566
                     /gene="sufT"
                     /locus_tag="PLB01_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HQQ78349.1"
                     /translation="MTPQVPTAHQLSRDCPAIRIPSGEPITLKAGTDVFLTQSLGGTL
                     TVQAPALGGLFRISGADGDALGLSAGSEDSPAAAAGPVSEDAVWEQLRTVYDPEIPVN
                     IVDLGLVYDLRVLPAEKGGTRVEVKMTLTAPGCGMGPAIASDAQSRVARVPGVKEAEV
                     QLVWDPPWSAEMMTPEGKRILGIA"
     gene            complement(6571..6912)
                     /locus_tag="PLB01_13430"
     CDS             complement(6571..6912)
                     /locus_tag="PLB01_13430"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HQQ78350.1"
                     /translation="MTEPLRKTFTFDEARALLPAVREKTRRALEALDGLAAVADDDAE
                     SSRRRTERAVEGILNAWREELEALGIEVKGPWLVDFDSGAGYYCWKWPEESLEFFHGY
                     EEGFAGRVPLQ"
     gene            6952..>7932
                     /locus_tag="PLB01_13435"
     CDS             6952..>7932
                     /locus_tag="PLB01_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HQQ78351.1"
                     /translation="MNPLAKDAAAADASAVTFRRQLHARPETAFEEVETARLVEARMR
                     ARGLAVRTGLGKTGVAAVLDTGRPGRTVLLRADLDALPVAEKTGLPFASANGRMHACG
                     HDGHVAALDAAADLLLLDPPAGGRVVFAFQPAEEGAGGAAAMIADGVLDDPRPDAVFG
                     VHLWTSLPLGAAGVASGAMMAAVDEFRIDVTSPGGHAASPHETRDAVVAAALVVTALQ
                     TVVARRLSPLQPAVVSVTSVHGGSAFNVLPESVTLTGTCRSFDRDASERLAVLVPEIA
                     AHAASAAGCAAKTVYERRSGAVVNHPAEAARARRAADAVLELLQHAAGAEQ"
BASE COUNT         1081 a         2837 c         2743 g         1271 t
ORIGIN      
        1 ccgctccggc agcccgtcgc gcgcgttcgg gtccggcgtc cagacgagga cgcccgcgta
       61 gtcgagcgcg ttcgtgagca gcgtcgcggg cacgcggagc gggccggtca cgggagcgcc
      121 gtacgcgccc gacacgcgcg agccggcggg cacgtcgcgg atgcgtcccc cgaggttcgc
      181 ggggttctgc aggtatccga tcccgaacgg caggaacgcg acgcctgccg tgagccacgc
      241 cgcgagaacg agcgcgcggt tctccctcgc gcggaggagc gaaagcgaga agacgccgag
      301 tcccgccgcc acggcccacg acgaggaatg cgtatggagc gagaggccga gcgctgcgcc
      361 cgcgaggagg gcgaacgcga cccgccgcgt ccggagcgcc gcgagcgtcg cggcgcccgc
      421 gacgagcgcg agcgggatga gcgcggcggc cgtgtagccc cagcgcgaga agacgagcgg
      481 ccagagaccg agcgcgacgg ccacggtcgc gagggacgcg gcgaggccgc ccgagacctc
      541 ccacgcgagc cacgcggccg ccgggatcgc gaggactccc gggaccgcgg acagggcgcc
      601 gaaacccgct tcgccgcgcc cgaagacgcg gaacacgagg tcgtagaagt ggaggtacac
      661 gaacgagacg agctcgcggt tgcccgccgc ggcggggtcg gcgtggagcg gcgcgccgcc
      721 gatccacggc aggccgtcgg gacgctcgag gagcgcgcgg gcctcgaaag gcgggtcgaa
      781 ccagagcccc ggcgggatcc actccggaaa ggcgaagcgc gaggcgatgc cgatcccggc
      841 ggcgaggagc gcgatcgcga cgacgccgcg cgtcgcccgc atcgcacgga ggtccgtcgc
      901 gcgctcgagg cgctccgtcg cgcggacgag cgccgcgaag agcacgccgg ccaggagcgc
      961 gagcgcgacg ttcttgagcc agagcagcgt gacgaggagc cacgtctccc tcgtgaagaa
     1021 gaagagcacc gcccagcggt cctcgcgcgc gaggaagacg aacgtctcga ccgcgacgaa
     1081 ggcgctgaac gcccgtctca cctcagctac agccgctcgt cgctccgcag ttcaggcact
     1141 tgtagcaggc gccgccgcgc accatcaggc tcccgcactc gtggcagctc ggggcgtccg
     1201 cgctgttctc gaacgtcagt ttcgaggccg gggccagggg caccgcgaaa ccgatcgcgc
     1261 gcggctccgc ggcctcggcc ggcgtctccg accggatgcc ggcgatcgcc tggaacgccg
     1321 ccgtgatcgg cgccatcgcc gcctcggcga cgggcgcgag cgtcgtgacc gacgccgtcg
     1381 ccttgtagcc ggacgccgac gtcgccgggc cgggctcctc gcgcgagatg atcccgacct
     1441 tctccttgag gtccgcggtc aggaacttcg acgcgagcca gcggaatatg tagtcgacga
     1501 ggctcttcgc gatcgggatc tcggggttct tcgtgtagcc cgcgggttcg aagcgcatgt
     1561 ggctgaactt ctccacgagc gcctcgagag gcacgccgta ctggagcgtc agggagatcg
     1621 cggtcgcgaa cgcgtccatg aggccggaga tcgtcgagcc ttccttcgcc atcgtgagga
     1681 agatctcgcc gggcatcccg tcctcgtaga ggccgaccgt cacgtagccc tcgtggcccg
     1741 cgacggagaa cttgtgcgtg atcgcgcggc gctcgtccgg cagcttgtgc cggcgcaggg
     1801 cgggggcgat cgcaacgggc gcggcggcgg cgatcgtgtt gccgcgcgcg tcgcgcgtgc
     1861 ccttcacccc gtcgtcgttc ttggacgccg acgtgttgag cggctgcgtc ctcttgcagc
     1921 cgtcgcggta gatcgcgatc gccttgaggc cgagcttcca gccctgcatg tagacgtccg
     1981 cgatctcctc gacggtcgcg tcgttcggca tgttcacggt cttgctgatg gcgcccgaga
     2041 ggaacggctg gaccgccgac atcatcctca cgtggcccat cggggcgatc gagcgcgtgc
     2101 cgccgagggc cttgaacgcg cagtcgaaga cggggaggtg ctcgctcttc aggcccggcg
     2161 ccccctcgat cgtgcccttc tcgtcgatgt ggcggacgat cgcgtgcatc tcggcgtccg
     2221 agtagccgag gcgcgtgagc gcgtggggga ccgtctggtt gacgatcttg atcgtgcccc
     2281 cgccgacgag tttcttgtat ttcacgagcg cgaggtccgg ctccactccc gtcgtgtcgc
     2341 aatccatcat gaaaccgatc gtgcccgtgg gcgcgaggac ggtcgcctgc gcgttgcggt
     2401 acccgtgctg ccgcccggac tcgagggtga ggtcccacac ggcgcgctgg gcctcccaga
     2461 ggtccttcgg gacgcccggc ctcgcgacct cgtacgccgc gtcgcggtgc atcgagatga
     2521 cctcgagaaa cgcatcgcgg ttcggcggat agcccgcgaa gggccccatc tccgaggcga
     2581 tcttcgagga cgtgaggtag gcctgcccgt gcatgaggct cgtgaccgcg gcggcgtagt
     2641 ggcggccctc gtcgctgtcg tacgggaggc cgttgtacat gaggagcgcg ccgaggttcg
     2701 cgtagccgag accgatcggc cggaagtcgt gcgagttctt ctcgatcgcc ggcgtcggat
     2761 acgaggcctc cgagacgagc atctcctggg ccgcgaagac gacgtccacg gcgtgctgga
     2821 acttctcgat ctcgaacccg ccgtcctccc gcgcgaactt catgaggttc agcgacgcga
     2881 ggttgcaggc cgagtcgtcg atgaacatgt actccgaaca cgggttcgac gcgttgatcc
     2941 ggtgcgtcgc cttgcacgga ttccacttgt tgatgagcgt gtcgtactgc atgccggggt
     3001 cgccgcagac ccaggccgac tcggccatct tcacgaggag ctctttcgcg tcgaacgtcc
     3061 ccatgggctc gccggtcgtg acggccttcg tcgtgtgcgt gccgccggac tcgacggcct
     3121 tcatgaactc gtccgtgacg cgcacggagt gattcgcgtt ctggaaggcg atgttgctgt
     3181 agacctcgcc gttgaacgag ccgtcccatc cggcgtcgat gaggtcccag gccttcttct
     3241 cttccttctc cttgcagagg atgaactcct cgatgtcggg gtggtcgacg ttgaggatga
     3301 ccatcttcgc cgcccggcgc gtcttgccgc ccgacttgat cgcgcccgca aagctgtcga
     3361 agcccttcat gaaggagacc gggccggagg caatgccgcc cgacgagagc gactcggtcg
     3421 acgagcgcag gctggagagg ttcgagcccg tgcccgagcc gaacttgaag agcatgccct
     3481 cggtcttggc gaggtccagg atcgaggaca tcgaatcgcc gaccgagttg atgaagcacg
     3541 cggaggcctg cggcttcggc tcgacgccga cgttgaacca cacgggcgag ttgaagctcg
     3601 ccgcctggtt cacgaggatg tgcgtgagct cgtccgagaa cgcgtcgcgg tcggcggcgg
     3661 tgcggaagta gccgcccttc gcgccgaagc ccgtgatcgt ctcgacgacc cggccgatga
     3721 gctggcggac cgaccgctcg cgctgcggcg tccccggctt cccggagaag tacttctggg
     3781 cgacgatgtt cgtcgccgtc atcgaccacg ccttcgggac ctcgacgtcc ttctgctcga
     3841 agaacacctc gcccttctcg ttcgtgatcg cggccgtgcg cagctcccac tcgatcgcgt
     3901 cgtaggggtg cgtcacgccg tccgtgtaga cgcgtcggaa cgtgagcccg cgggcgcccg
     3961 atcggggggc cgcgccgttc gccttctccg tcctcgttcc ggccttcgtc tcgcgcgcat
     4021 tcatgttgct cattgccggg aaagcctccc tctcacttcg gatcacgtga gtggtttgtc
     4081 ctgtttttgc cgccacccgg cggcacctcg ggcccttcgt ctgaccggga aagacgactg
     4141 ttcgcgatcg ccggttcgtt gtcaggtctg tcggacaagg tagtgccgct gagatcgctt
     4201 ttcaagggga aaacgcaagg gatagtgttt ttctttcggg gagccactag atggtggggt
     4261 tttcaaccgc ctgtggggaa tacgcaaacc ctctgactcg tttgacttcc acgggcgttc
     4321 gcggccatat tccacaggcg gcgcgcgcgc cgtcggaggt ttctttcatg tccagcgctc
     4381 ccgagaccgc cacgcaggtt gcttccccgt ccgtcttcct cctcagcgtg acggatcgcg
     4441 ccgtcgcgaa ggtgaaagag ttcgccgcca agatgcccga ggccgcaaac aagcccctgc
     4501 gcgtcttcat ccagggcggc ggctgctccg gcttccagta cggcttcacg ttcgacgaca
     4561 agcggccgac ggaccacgtc ctcgccgtga aggacatcga ggtcgtcgtg gacccggcga
     4621 gcgcgctcaa gctccacggc tccaccgtgg acttcgtcga ggacatgcgc ggggcgggct
     4681 tcgcggtcga gaatccgaac gcgacgtccg gctgcggctg cggaaaatcc ttcagctgac
     4741 gatcgtgaac gggaaacccg tcatgcgggt ttccctcacc cttccgctcg gacgcttcgc
     4801 gtcctgcgca ggcttgtgaa gaacccctcg atcctgaaga tcgtcttcct gacggtcttc
     4861 atcgaccttc tcggcttcgg gatcgtgatc ccgcttctgc cgctctacgc cgagaagtac
     4921 cacccgtcgc cgctcgtctt cggcctcctc atgtcgagct attccgcgat gcagttcctg
     4981 ttcggccccc tcctcggccg actctcggac cgttacgggc ggcggcccgt catcatcttc
     5041 tcgctcgcgg gcacggtggt cggctacgtc ctcttcgcgt tcgcgcgctc gcttccgatg
     5101 ctcttcgtcg cgcgcctcct ccccggcgcg acgggcggaa acatcgggac cgcgcaggcc
     5161 gtcatcgcag attcgacggc gcccacggag cgcgcgcgcg gcatggcgat ggtcgggatc
     5221 gcgttcggcc tcggcttcat cttcgggccc gcgatcggcg gcttcgccgt gcacctcgga
     5281 gaggcggcgc cggggctgtt cgccgctggc ctctctctcg tcgcgtgcgt gtgggccttc
     5341 ttcaagctgc ccgagacgcg ccccgcgggg aaggtcgcgc cctccggccc gctcttctcg
     5401 gcgggcccgc tcctcgcggc gttccgccgg cccgagatcg gcgcgctcat gctcctttcc
     5461 ttcgtcacga cgacggcctt cgcgaacttc gagtccacgt tcgcgcagtt cctctcgctc
     5521 cggctcgggg cggggccgtc gacggtcgcc tggttcttcg tcttcgtcgg cgtctgctcg
     5581 gtcgtcgtcc agggcggcct cgtgcggcgc ctcgcgccgc gggtcggcga ggcccgcctc
     5641 gtgatggcgg gctgcgtctt cctgatcgcg ggtttcctcc ttctccggct cgggacgacc
     5701 ccgccgcggc tcctcgcggc catcgccgtg atcgcgctcg gcatcgggat cacgacgccg
     5761 acgctgtcgt ccctcgtttc gaggcgcacg gcggcgaccg agcagggcga ggtcctcggg
     5821 gcgtaccagt ccatggcctc cctcgggcgg gtcttcggcc cgttcgcggg cgagaacctg
     5881 tacctgcgcg tggggccgga cgcgccgcac tgggccgccg ccgcgctaca gggcggcgcc
     5941 ctcgtccttt ccgccgccgg tctcctgaga gagaatgccg ccgcgccctc gcgcgcagag
     6001 gagtcccccc gatgacgccc caggttccca cggcgcacca gctctcgcgc gactgccccg
     6061 ccatccggat cccgtcgggc gagcccatca cgctcaaggc cggaaccgac gtcttcctca
     6121 cgcagtcgct cggcggaacg ctcaccgtgc aggcgcccgc tctcggcggc ctgttccgga
     6181 tttcgggagc ggacggcgac gccctcggcc tctcggccgg ttccgaagac tccccggccg
     6241 ccgcggcggg cccggtcagc gaggacgcgg tgtgggagca gctccggacc gtgtacgacc
     6301 ccgagattcc cgtgaacatc gtggatctcg gcctcgttta cgacctgcgc gtcctccccg
     6361 cggaaaaggg cggcacgcgg gtcgaggtga agatgacgct cacggctccg ggctgcggga
     6421 tgggtccggc gatcgcgtcg gacgcgcagt cccgcgtcgc gcgggtgccg ggcgtgaagg
     6481 aagccgaagt ccagctcgtg tgggacccgc cctggagcgc ggagatgatg acgcccgagg
     6541 gaaagcggat cctcgggatc gcctgacccg ctactgcagg ggcacgcggc cggcgaagcc
     6601 ctcttcgtac ccgtggaaga actcgaggga ctcctcgggc cacttccagc agtaatagcc
     6661 ggcgcccgag tcgaagtcca cgagccacgg acccttcacc tcgatcccga gggcctcgag
     6721 ctcctcgcgc cacgcgttca ggatcccctc gacggcgcgc tccgtccgcc gccgggacga
     6781 ctcggcgtcg tcgtcggcga cggccgcgag gccgtccagc gcctcgaggg cgcgccgcgt
     6841 cttctcgcgc acggccggga gaagcgcacg ggcctcgtcg aacgtgaacg tcttccggag
     6901 gggctcggtc acggcccgag gatagcgcgg tccccggcct atgatcccgg cgtgaatccg
     6961 ctcgcgaagg acgcggccgc cgcggacgcc tccgccgtca cgttccggcg ccagctccac
     7021 gcccgcccgg agaccgcgtt cgaggaagtc gagaccgcgc gcctcgtcga ggcccgcatg
     7081 cgcgcgcgcg gactcgcggt gcgaaccggc ctcgggaaga ccggcgtcgc ggcggtcctc
     7141 gacaccggcc ggccgggccg gacggtcctt cttcgcgccg acctcgacgc gctcccggtc
     7201 gccgagaaga ccgggctgcc gttcgcgtcg gcgaacggcc ggatgcacgc ctgcgggcac
     7261 gacgggcacg tcgccgctct cgacgcggcg gccgaccttc tgctcctcga cccgcccgcg
     7321 ggcggccgcg tcgtcttcgc gttccagccg gcggaggagg gcgcgggcgg cgcggccgcg
     7381 atgatcgccg acggcgtcct cgacgacccg cgccccgacg ccgtcttcgg cgtccacctc
     7441 tggacgtcgc tcccgctcgg cgcggcgggc gtcgcctccg gggcgatgat ggccgccgtc
     7501 gacgagttcc ggatcgacgt cacgagcccc ggcgggcatg cggcctcgcc gcacgagacg
     7561 cgcgacgccg tcgtcgcggc ggcgctcgtc gtcacggcgc tccagaccgt cgtcgcgcgg
     7621 cgtctgtcgc ccctccagcc cgccgtcgtg tccgtcacgt ccgtgcacgg cggctccgcg
     7681 ttcaacgtcc tcccggaatc cgtgacgctg acgggcacct gccgctcgtt cgatcgcgac
     7741 gcgagcgagc gcctcgcggt gctcgtcccg gagatcgccg cccacgccgc ctcggctgcg
     7801 ggctgcgcgg cgaagaccgt ctacgagcgg cggagcgggg ccgtcgtgaa ccaccccgcc
     7861 gaagccgcgc gcgcacgccg cgccgcggac gccgtcctcg aactccttca gcatgcggcc
     7921 ggcgccgaac ag
//
LOCUS       DAOMFY010000180         7904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2865_length_7904_cov_8.793095, whole genome shotgun sequence.
ACCESSION   DAOMFY010000180 DAOMFY010000000
VERSION     DAOMFY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2865_length_7904_cov_8.793095"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PLB01_13440"
     CDS             <1..1191
                     /locus_tag="PLB01_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQQ78352.1"
                     /translation="RRDELGAVIAREAGKPLKAARTEVERAGATFSAAAAALEAWKGD
                     MLPLDVNAASVSRWGVVRRVPLGPVLAITPFNFPLNLTAHKVAPAVAVGAPVVQKPAS
                     QTPLSALALQAIVREAGWPVDAYAVLAISGREAEALVTDPRLPVVSFTGSGAVGWRLR
                     EIAPRKRVGLELGGNAAVVVHADADLDDAASRVAAGAFGYAGQSCISVQRALVQEDVF
                     ETFRAKLLAKTAALAVGDPLDAKTDVGPMIAPEEAVRAAAWIEEAVAGGARVLAGGTR
                     SGSLVLPTILAGTTRDMKVEAQEVFAPIVTLASYATWEEALRRVNDSKYGLQAGVFTN
                     DLSRIQRAFDVLEVGAVLVNDVPTWRADRMPYGGVKESGIGREGPAYAMEEFTEPRLL
                     VLRP"
     gene            1209..1652
                     /locus_tag="PLB01_13445"
     CDS             1209..1652
                     /locus_tag="PLB01_13445"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQQ78353.1"
                     /translation="MEGVTTFSISASWDPSAKEGRLANADGSFAAVHTGAAALDGKGG
                     TANPEELLLAAVTACFLQTWAIFVEKLKLPIASPRLDATCALEKDPAGGFHVTRIDLF
                     PHVPAALLEERKADVEKTLALAEKYCIVSKAVRGSVVLSVALRSV"
     gene            1688..1993
                     /locus_tag="PLB01_13450"
     CDS             1688..1993
                     /locus_tag="PLB01_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HQQ78354.1"
                     /translation="MPTSVRLDEKTGRLVERLAREADQTKSEVIREAITTYGRQRKTR
                     RAGNLYEAIAPYVGVATGGPPDLSERTGEKYHEALLGREKKAAASGGPGHPRRTKTR"
     gene            1990..2394
                     /locus_tag="PLB01_13455"
     CDS             1990..2394
                     /locus_tag="PLB01_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HQQ78355.1"
                     /translation="MILTDAGPLVALVHAGDRNHERCVAATRSLQGPMATVWPALTEA
                     MHLLRFSWPAQRALWSMLEEGTLRLLPLDESDAPRIGELMRKYRDLPMDLADAALVRV
                     AEREKLRRVFTLDRRDFSLYRPLRLGRFLLVP"
     gene            complement(2391..4481)
                     /locus_tag="PLB01_13460"
     CDS             complement(2391..4481)
                     /locus_tag="PLB01_13460"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQQ78356.1"
                     /translation="MILLAAVLAAQALTLEKVSESRMPVAYTWRDATRVTWVESGGPD
                     APAALWQADAAGGKKAKLLDAPAVKARDGKEKKLSLRGMAWTPKGDSFAVPFDNDLWL
                     VTPAGEARRLTNDPEEETLPAWSPDGARLAYVKKHDIWVADPATGKETRLTKTGSDTV
                     LNGSLDWVYGEELASRRAKGSFFWSPDSSAIAYLALDQARVPTYPIVDYTPVNGKLEA
                     QRYPKAGDPNAVPSVHVVDVAGTETASFAPSPDDVYVAPEMAWTQDGKSLCFLLLDRP
                     QTHLGVWLLPRAGGAPKRLLEESDAAWINAISPPHFLGDGSFVFLSERSGFFHLYRHA
                     ADGKVRNAITKGAWMVDGPWAVDEKMGTVWFRATTEDPRERQVFVAKLDGSSMVRVTG
                     EPGVHTPVFAPGAAYFADSFSSTTAPPKTVVRSASGAVAAPVDEKLHPLADFDLGSVE
                     MGSFTGSDGTLFYTRLVKPSNFDPAKKYPVVVYVYGGPHTQLVQNAWAAGMDRYFASK
                     GFLYWSLDGRGSWGRGHAFETPLLRNMGAQELKDQLEGVAELKKRPYVDGARIGITGW
                     SYGGYMTLYAATHAGATFKCAAAGAPVVDWTLYDSIYTERYMKAPKDNAEGYKASSPL
                     RTAKDLGTKLVIFHGTSDDNVHMQNTMKFADELMKARKDFVFVPLPRQAHGPRGEALL
                     YRNQRLAEWFEQNL"
     gene            complement(4478..6037)
                     /locus_tag="PLB01_13465"
     CDS             complement(4478..6037)
                     /locus_tag="PLB01_13465"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ78357.1"
                     /translation="MKYTLETVLLASACALSGTLGAAGAIPARPEQLAFKPLAWAPPA
                     AKDHRVVLKKSGVVAYLAENHDLPLVNVQILLKGGTYLNPPGREGLAETTGWLLARGG
                     TKKRKAEDLEERLAFLAAQLNPEQTFGRPGAARPTGYLDDRGVVTLNLLSKDLDEGLA
                     ILREVLTEPAFQEDRLKLRKEQLLAEMKQRNDSSASIEMRERSVLLTGADFYTNRWET
                     KASVEALTQADLAAFHARWIAPRNMIVSAAGDFRKADMATKLDALLVNWPFKGEAAPP
                     VPKPSHALPAGTFLVDKDVNQGRVSILLPGLMRTDPDFVPASVMNDILGGGGFTSRIT
                     NRVRSDEGLAYSAGSALVGGIWYPGRFGAAFQSKVRTSAYASQIVLEEMKKLRDGDVS
                     DEELETAKRAFVDTLPRRFATPVQVVDGLADEEFTGRYASDPGYWAAYQSRVEKVTKT
                     DIARVAKRLLKPESAAILVVGRKSDLLNPDPKHPVAFADLTGGKLVELPLRDPLTMQP
                     VAKAAAPGEKK"
     gene            complement(6040..7623)
                     /locus_tag="PLB01_13470"
     CDS             complement(6040..7623)
                     /locus_tag="PLB01_13470"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ78358.1"
                     /translation="MRNRIILATALAALALPVRAQELKVEEHVLPNGMKLLLVPRADE
                     PSVAAGWVAHVGSANERPGITGISHLFEHMMFKGTRTIGSRDPARDQKLIEEQERVRG
                     LMRDEDAKLRAALRRGEIDDITKPENKTARYKELDKEFDALVKEQRDLLVKNEFDRIY
                     TKNGASGMNASTNTDLTQYFITVPKNKLELWFWMESDRLSAPVFREFYSERDVVFEER
                     RLRTESTPTGRLEEAFEEMFWHGHPYSWPVVGYASDIPAITKAQADEYYALFYAPNNI
                     TAVLVGDFDPKEALALAATYFGRIPRGKNPPPEMTLLPPKWEAEMRMNGEADTNPRVE
                     VNWHTVPFGHKDSYPLSVLAQLLNGRTGRLYKALVAGENPVATTVQAGASARKYAGEF
                     GIEAQVKDGRAPEETERAIYAELDRLKKDLVPAEELQKVKNNSAAQAFRSMQTNMGIL
                     YSLMGNDGRGDWKEFREGPKKIQAVTAEDVRRVAQTYLTKENRGVATYTRKGGAAPRR
                     PGRPGGPPPGAAAAGTETR"
     gene            complement(7684..>7904)
                     /locus_tag="PLB01_13475"
     CDS             complement(7684..>7904)
                     /locus_tag="PLB01_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78359.1"
                     /translation="TPAARDAGSEALAALVADVLAGTSALGIGRPADMSVLSDDPAAP
                     DPARRAAIKVVEEVRKGRTVWRRDPTRR"
BASE COUNT         1112 a         2686 c         2842 g         1264 t
ORIGIN      
        1 cggcgcgacg agctgggggc cgtgatcgcg cgcgaagccg gcaagcccct gaaggcggcc
       61 cgcaccgagg tggagcgcgc cggcgcgacc ttctccgcgg cggcggcggc cctcgaggcg
      121 tggaagggcg acatgctgcc gctggacgtg aacgcggcgt cggtttcgcg ctggggcgtc
      181 gtgaggcgcg tgccgctcgg gcccgtcctc gcgatcacgc cgttcaactt cccgttgaac
      241 ctcacggcgc acaaggtggc gcccgcggtc gccgtcggcg cacccgtcgt ccagaagccc
      301 gcgtcgcaga cgccgctctc ggccctcgcg ctccaggcga tcgtccgcga ggccggctgg
      361 cccgtggacg cgtacgcggt cctcgcgatc tccggccgcg aggccgaggc gctcgtcacc
      421 gatccgcggc tgcccgtcgt gtcgttcacg ggctcggggg ccgtcggctg gcggctgcgc
      481 gagattgcgc cgagaaaacg cgtcggactc gagctcggcg gcaacgccgc cgtggtcgtc
      541 cacgcggacg cggacctcga cgacgcggcc tcccgcgtcg cggccggcgc gttcggctac
      601 gcggggcagt cgtgcatctc cgtccagcgc gcgctcgtcc aggaggacgt cttcgagacg
      661 tttcgcgcaa agctcctcgc gaagacggcg gcgctcgccg tcggagaccc gctcgacgcg
      721 aagacggacg tcgggccgat gatcgcgccc gaggaagccg tgcgcgcggc ggcgtggatc
      781 gaggaggcgg tcgcgggcgg cgcgcgcgtc ctcgcgggcg ggacgcggtc cggctccctc
      841 gtcctcccca cgatcctcgc gggcacgacg cgcgacatga aggtcgaggc gcaggaagtc
      901 ttcgcgccga tcgtgacgct cgcgtcctac gcgacgtggg aggaagcgct ccggcgcgtg
      961 aacgactcga agtacggcct gcaggcgggc gtcttcacga acgacctctc ccgcatccag
     1021 cgcgcgttcg acgtcctcga ggtcggcgcg gtcctcgtga acgacgtccc gacctggcgc
     1081 gcagaccgca tgccgtacgg cggcgtgaag gagtcgggca tcggacgcga gggcccggcg
     1141 tacgcgatgg aagagttcac ggagccgcgg ctcctcgtgc tccggccctg aggcccgaaa
     1201 ggaatcccat ggaaggcgtc acgacgttct cgatctccgc gtcctgggat ccctcggcga
     1261 aggagggacg cctcgcgaac gccgacggct cgttcgcggc ggtgcacacc ggcgccgcgg
     1321 ccctcgacgg gaaagggggc acggcaaacc ccgaggagct tctgctcgcg gcggtcacgg
     1381 cgtgcttcct ccagacgtgg gcgattttcg tcgagaagct gaagctgccg attgcctcgc
     1441 cgcgcctcga cgcgacgtgc gcgctcgaga aggaccccgc gggcgggttc cacgtcacgc
     1501 ggatcgacct cttcccgcac gtcccggccg cgcttctcga agaacgcaag gccgacgtcg
     1561 agaaaacgct cgcgctcgcc gagaagtact gcatcgtctc gaaggccgtg cggggctccg
     1621 tcgtgctgtc ggtggctctc aggtcggtct aggcatgtag tacaatctcg taggacgagg
     1681 tgggcacatg cctacgagcg tgagactcga cgaaaagacc gggaggctcg tggaacgcct
     1741 ggcccgggaa gccgaccaga cgaagtccga ggtgatccgc gaggcgatca cgacgtacgg
     1801 ccgtcaacga aagacgcgcc gggcgggaaa cctctacgag gcgatcgccc cgtatgttgg
     1861 cgtcgcgaca ggcgggccgc cggacctgtc ggagaggacg ggcgagaagt atcacgaggc
     1921 actcctcgga cgcgagaaga aggcggccgc gagcggcggc ccagggcacc cccggcggac
     1981 gaagacgcga tgatcctcac ggacgccggg cctctcgtcg cgctcgtcca cgctggcgac
     2041 cgcaaccacg aacggtgtgt cgcagccacc cggtcgctgc agggcccgat ggcgaccgtc
     2101 tggccggctc tgacggaggc gatgcacctg ctccgcttct cgtggccggc ccagcgtgcg
     2161 ctctggtcga tgctcgagga gggcacgctg aggctgctcc ccctcgacga gtccgacgcc
     2221 ccccggatcg gggaactgat gcggaagtat cgcgatctgc cgatggacct cgcggacgcg
     2281 gctctcgtcc gcgtcgcgga aagagaaaag ctccggcgcg tcttcacgct cgacaggcgc
     2341 gatttctctc tctatcgacc actccgcctc gggcgcttcc tgctcgttcc ctagaggttc
     2401 tgctcgaacc actccgcgag ccgctggttg cggtagagga gcgcctcgcc gcgcggcccg
     2461 tgcgcctggc gcgggagcgg cacgaagacg aagtccttcc gcgccttcat gagttcgtcg
     2521 gcgaacttca tcgtgttctg catgtgcacg ttgtcgtccg acgtcccgtg gaagatcacg
     2581 agcttcgtcc cgaggtcctt cgccgtgcga agcggggagg aggccttgta cccctcggcg
     2641 ttgtccttcg gcgccttcat gtagcgctcg gtgtagatcg agtcgtagag cgtccagtcg
     2701 acgacgggcg cgccggcggc ggcgcacttg aacgtcgcgc ccgcgtgcgt cgcggcgtag
     2761 agcgtcatgt acccgccgta cgaccagccc gtgatcccga tccgcgcgcc gtcgacgtag
     2821 ggccgcttct tcagctcggc gactccctcg agctggtcct tcagctcctg cgcgcccatg
     2881 ttcctcagga gcggcgtctc gaaggcgtgc ccgcgccccc acgagccgcg cccgtccagg
     2941 gaccagtaga ggaagccctt cgacgcgaag tagcggtcca tccccgcggc ccacgcgttc
     3001 tggacgagct gcgtgtgcgg gccgccgtag acgtagacga cgacgggata cttcttcgcg
     3061 gggtcgaagt tcgagggctt cacgagccgc gtgtaaaaaa gcgtcccgtc ggagccggtg
     3121 aacgagccca tctcgacgct cccgaggtcg aaatcggcca gcggatgcag cttctcgtcg
     3181 acgggggcgg cgaccgcgcc cgaggcggaa cggacgaccg tcttcggcgg cgccgtcgtg
     3241 ctcgaaaacg agtcggcgaa gtacgcggcg cccggcgcga aaacgggcgt gtgaacgccc
     3301 ggctcgccgg tgacgcggac catggacgag ccgtccaatt ttgcgacgaa gacctgccgt
     3361 tcgcgcgggt cttcggtcgt cgcccgaaac cagacggtcc ccatcttctc gtcgaccgcc
     3421 caggggccgt ccaccatcca cgcgcccttc gtgatcgcgt tccggacctt gccgtccgcg
     3481 gcgtggcggt agagatggaa gaaaccgctc cgctcggaca ggaacacgaa cgatccgtcc
     3541 cccaggaagt gcggaggaga gatcgcgttg atccacgcgg cgtcgctctc ctcgaggagc
     3601 ctcttcggcg cgccgcccgc gcgcgggagg agccagacgc cgaggtgcgt ctgcggccgg
     3661 tcgaggagga ggaagcacag ggacttcccg tcctgcgtcc atgccatctc cggggcgacg
     3721 tagacgtcgt ccggcgacgg cgcgaacgag gccgtctcgg tcccggcaac gtccacgacg
     3781 tggacggacg ggaccgcgtt cgggtcgccg gccttcgggt agcgctgcgc ctcgagcttc
     3841 ccgttcaccg gcgtgtagtc gacgatcggg tacgtcggga cgcgcgcctg gtcgagcgcg
     3901 aggtaggcga tcgcggacga gtcgggggac cagaagaacg accccttggc gcgccggctc
     3961 gcgagctcct cgccgtacac ccagtcgagc gacccgttga ggaccgtgtc cgagcccgtc
     4021 ttcgtgagcc gcgtctcctt tcccgtcgcg gggtcggcga cccagatgtc gtgcttcttc
     4081 acgtacgcga gacgcgctcc gtccggcgac cacgccggga gcgtctcctc ctcggggtcg
     4141 ttcgtgaggc gcctcgcctc gccggcgggc gtgacgagcc agaggtcgtt gtcgaacggg
     4201 acggcgaacg agtccccctt cggagtccac gccatgccgc gcagggaaag cttcttctcc
     4261 ttgccgtcgc gggccttcac ggcgggcgcg tcgaggagct tcgccttctt tccgccggcg
     4321 gcgtcggcct gccagagggc agccggagcg tcggggccgc cggactcgac ccacgtgacg
     4381 cgcgtcgcgt cgcgccacgt ataggcgacc ggcatgcgcg actcggagac cttctcgagc
     4441 gtgagggcct gggcggcgag gaccgcggcg aggaggatca cttcttctct ccgggggcgg
     4501 cggccttcgc gacgggctgc atcgtgagcg ggtcgcggag cggaagctcg acgagcttgc
     4561 cgcccgtgag gtccgcgaag gcgacgggat gcttcggatc ggggttgagg aggtcggact
     4621 tgcggccgac gacgaggatc gccgcggact cgggcttgag cagacgtttt gcgacgcgcg
     4681 cgatgtccgt cttcgtgacc ttctcgaccc tggactgata cgcggcccag tacccggggt
     4741 cggacgcata gcggccggtg aactcctcgt ccgcgaggcc gtcgacgacc tgcacgggcg
     4801 tcgcgaaccg gcgggggagg gtgtccacga aggcgcgctt cgccgtctcg agctcctcgt
     4861 ccgagacgtc gccgtctcgc agcttcttca tctcctcgag gacgatctgc gaggcgtagg
     4921 ccgacgtccg caccttcgac tgaaacgcgg cgccgaagcg acccgggtac cagatgccgc
     4981 ccacgagggc ggacccggcc gagtaggcga ggccttcgtc ggagcggacc cggttcgtga
     5041 tgcggctcgt gaaaccgccg ccgccgagga tgtcgttcat gaccgaggcg gggacgaagt
     5101 cggggtccgt ccgcatcagg ccggggagga ggatcgagac gcggccctgg ttcacgtcct
     5161 tgtccacgag gaaggtgccg gccggaagcg cgtgcgacgg cttcgggacg ggcggcgccg
     5221 cctcgccctt gaacggccag ttcacgagca gcgcgtcgag cttcgtggcc atgtcggcct
     5281 tccggaagtc gccggccgcc gagacgatca tgttgcgcgg cgcgatccag cgggcgtgga
     5341 acgccgcgag gtcggcctgc gtgagggctt cgacggacgc cttcgtctcc cagcggttcg
     5401 tgtagaagtc ggcgccggtg aggaggacgg agcgttcccg catctcgatc gacgcggacg
     5461 agtcgttgcg ctgcttcatc tccgcgagga gctgctcctt ccggagcttc agccggtcct
     5521 cctggaacgc gggctccgtg aggacctcgc gcaggatcgc gaggccctcg tcgaggtcct
     5581 tcgagaggag gttgagcgtg acgacgccgc ggtcgtcgag gtatccggtc ggccgcgcgg
     5641 cgccggggcg tccgaaggtc tgctcggggt tgagctgggc ggcgagaaac gcgaggcgct
     5701 cctcgaggtc ctcggccttg cgcttcttcg tgccgccgcg cgcgaggagc catcccgtcg
     5761 tctcggcgag cccttcccgg cccggcgggt tcaggtacgt cccgcccttc aacaggatct
     5821 ggacgttcac gagcgggagg tcgtggttct ccgcgaggta cgccacgacg ccggacttct
     5881 tcaggacgac gcggtgatcc ttcgcggcgg gcggcgccca cgcgagcggc ttgaacgcga
     5941 gctgctcggg ccgggcgggg atcgcgccgg cggccccgag agtgccggag agagcgcagg
     6001 ccgaggcgag gaggacggtt tcgagcgtgt acttcatgac tacctcgtct ccgtgccggc
     6061 agctgccgcg cccgggggcg ggcccccggg gcggcccgga cgccgcggag cggcgccccc
     6121 tttccgtgtg tacgttgcga cgccgcggtt ttccttcgtg agatacgtct gcgcgacgcg
     6181 ccggacgtcc tcggccgtga cggcctggat cttcttcggc ccttcgcgga actccttcca
     6241 gtcgccgcgg ccgtcgttcc ccatgaggct gtagaggatg cccatgttcg tctgcatcga
     6301 acggaacgcc tgcgcggcgg agttgttctt caccttctgg agctcctcgg ccggaacgag
     6361 gtctttcttc aaacggtcga gctcggcgta gatcgcgcgt tccgtctcct ccggggctcg
     6421 cccgtccttc acctgagcct cgatgccgaa ctcgcccgcg tacttgcgcg cggacgcgcc
     6481 cgcctggacg gtcgtcgcga cggggttctc acccgcgacg agcgccttgt aaaggcggcc
     6541 cgtacgtccg ttgaggagct gggcgaggac actgagcggg tacgaatcct tgtgcccgaa
     6601 gggaacggtg tgccagttca cctcgacgcg cgggttcgtg tccgcctcgc cgttcatccg
     6661 catctccgcc tcccatttcg gggggaggag cgtcatctcg ggcggcgggt tcttcccgcg
     6721 cgggatgcgc ccgaagtacg tcgcggcgag cgcgagggcc tccttcgggt cgaagtcgcc
     6781 gacgaggacg gccgtgatgt tgttcggcgc gtagaagagc gcgtagtact cgtccgcctg
     6841 cgccttcgtg atcgcgggaa tgtccgaggc gtacccgacg accggccagg agtacgggtg
     6901 gccgtgccag aacatctcct cgaaggcctc ctcgaggcgg ccggtcgggg tcgactcggt
     6961 cctgaggcgc cgttcctcga agacgacgtc ccgctcggaa tagaactcgc ggaagacggg
     7021 cgcgctcagg cgatccgatt ccatccagaa ccagagctcg agcttgttct tcggaacggt
     7081 gatgaagtac tgcgtgaggt ccgtgttcgt cgaggcgttc atgcccgacg cgccgttctt
     7141 cgtgtagatc cggtcgaact cgttcttcac gaggaggtcg cgctgctcct tcacgagggc
     7201 gtcgaactcc ttgtcgagct ccttgtagcg ggccgtcttg ttctcgggct tcgtgatgtc
     7261 gtcgatctcg ccgcgccgga gggcggcgcg cagcttcgcg tcctcgtcgc gcatgaggcc
     7321 gcggacgcgc tcctgctcct cgatgagctt ctggtcgcgc gccgggtctc tggacccgat
     7381 cgtcctcgtc cccttgaaca tcatgtgctc gaagaggtgg ctgatccccg tgatgccggg
     7441 gcgctcgttc gcgctgccga cgtgggcgac ccatccggcc gcgaccgagg gctcgtcggc
     7501 gcgggggacg aggaggagct tcatcccgtt cggcaggacg tgctcttcga ccttcagttc
     7561 ctgcgcgcgg acggggagcg cgagggcggc gagcgccgtc gcgagaatga ttctgtttcg
     7621 catgaaatct cctgtgaaca cggcgtgagg accgatccta cgccggtcgg cccccgtgga
     7681 gactcagcgg cgcgtggggt cccgccgcca gacggtgcgg cccttccgga cctcctcgac
     7741 gaccttgatc gcggcgcgcc gcgcgggatc cggggcggcc gggtcgtcgg agaggacgga
     7801 catgtcggcc gggcggccga tcccgagcgc ggaggtcccg gcgaggacgt ccgcgacgag
     7861 cgcggcgagg gcctcggaac ccgcgtcgcg cgccgcgggc gtcg
//
LOCUS       DAOMFY010000181         7765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2947_length_7765_cov_7.448119, whole genome shotgun sequence.
ACCESSION   DAOMFY010000181 DAOMFY010000000
VERSION     DAOMFY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2947_length_7765_cov_7.448119"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2028)
                     /locus_tag="PLB01_13480"
     CDS             complement(<1..2028)
                     /locus_tag="PLB01_13480"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ78360.1"
                     /translation="MKRAPTAAAILALIVSLAFSSPTAAQSFAVEKYKLANGMTVILY
                     PDHTLPVAAINIWYRVGSKDEPARRSGFAHLFEHLMFMGTERVPNGEFDRLMEASGGS
                     NNASTTSDRTNYYSSGPASLLPTLLWLDAERLEGLPRAMDQKKVDLQRDVVLNELRQS
                     YENQPYGRAELAIQQMLYPPSHPYHFPTIGTAEDLKAAVAADVKDFFATYYVPNNASL
                     VVAGDFDPAAIKPLVEKLFGGIPRGGEPPRKPVPPAKLDGVRRGAMYDKVQLPLVAFA
                     YHAPPALTPGDADCDLLASVLSDGKSSRLYKRLVIDDKLAASVSASNASAALGSVFRI
                     DVQARADADLGRIEKAVDEEIARLLAEGIKPGELAQRKETTELAKLSALQRLETRADL
                     LNFYEDAWGDPNGFKRDLDRYRNATPVSVLATAKQVLTADARVVYRVLPEQPKRAPSA
                     RDARPADGPDAAFRPTSPERFKLANGLAVQYWKTPDLPLTRIAAVFRPEGALDASAAR
                     AGLASLSAGMLDEGAGTRDALAFGEAVASLGGSFSTGASRQSIQASLTILSRNVGQGV
                     PLLADAVRRPRFSPADFERVKRLTLDEISQSEDEAPVVADRVAARALFGDAHPYAWPA
                     GGTPASVGALTLDEAKAAHAALVRPDAATLLVASSLPAAEIKAALEASFGDW"
     gene            complement(2137..2613)
                     /locus_tag="PLB01_13485"
     CDS             complement(2137..2613)
                     /locus_tag="PLB01_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQQ78361.1"
                     /translation="MRTRAPKTPTGANQIADLRRAGLRVTPARLAVLAALRARTAPAS
                     HADLASALEGAVSDRATVYRNLVELVKAGLARRRDLGDHVWRFEAAGARPGGKDHPHF
                     LCTSCGSLACLDTLKVSLRRGVRVPRALKDDAVEILVKGLCDECQGKRDRREEKIS"
     gene            2656..3363
                     /locus_tag="PLB01_13490"
     CDS             2656..3363
                     /locus_tag="PLB01_13490"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQQ78362.1"
                     /translation="MTDEKNDRRDFVKTAAVAAAGLAIKDAAAPSVAKGATPITLPAL
                     PWADTALEPFISKNTIGFHYGKHHKAYVDNLNNLVAGKPEADLPLEAIVKGAAGKADQ
                     AAVFNNAAQIWNHTFYWNSMRPKSDAKVPKQIADLIGASFGSHEEFVKQFSAAAMGQF
                     GSGWAWLVAEGGKLKVVKTPNAETPLTTSATPLLTLDVWEHAYYLDYQNRRKDYVAAV
                     VDNLLNWDFALKNLPKA"
     gene            3369..3827
                     /locus_tag="PLB01_13495"
     CDS             3369..3827
                     /locus_tag="PLB01_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQQ78363.1"
                     /translation="MNADLEARQAVVDTLTELFLATDRRDWPAVEGCFAETVTFDMTS
                     LAGGQPARLTPRDISSAWTQGLAPIESVHHQAGNFRVRIDGNEASAFCYAIAYHYRRV
                     VSGRNLRTFVGSYDFHLARDGAQRWRIDLFRFNIKFLDGNLDLHSEDAAG"
     gene            complement(3831..4808)
                     /gene="rfaD"
                     /locus_tag="PLB01_13500"
     CDS             complement(3831..4808)
                     /gene="rfaD"
                     /locus_tag="PLB01_13500"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HQQ78364.1"
                     /translation="MSTDTLLVTGAAGFIGARFVDSCNARGVPVVSVDRREAFTMRPE
                     HRRLDFGKILDIEELPGWLAGGRPALAGIVHMGAISATTELDEGLLKRLNVDYSKHLW
                     TEARRLGIPFVYASSAAVYGDGALGFDDDDALVPSLKPLNPYGESKRRFDLWALEEAA
                     AGRTPPTWAGFRFFNVYGFGERHKGPQASVVLHGYDQVKAKGEIRLFRSHRDGVAHGH
                     QSRDFVDVSDTVEAMWFALKRPITAGIYNVGTGKARTFLDLAKAVFASLAVPERIEFV
                     DTPVGIRERYQYFTEAKMDRLRKQGFARKPVALEDGVDWYIRRLESERA"
     gene            4869..5837
                     /locus_tag="PLB01_13505"
     CDS             4869..5837
                     /locus_tag="PLB01_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HQQ78365.1"
                     /translation="MTITSFVKHHFRHFNAATVVDAAEAWNGLLAGGGKMFLTMAGAM
                     STAEIGLSLAEMIRQDKVHAITCTGANLEEDVFNLVAHDHYVRIPHYRQLSPAEEEAL
                     HARHLNRVTDTCIPEEEAIRRIEKAVLEEWQAADRAGERRFPHEFLYRILLSGKLKES
                     YQIDPKDSWLLAAAQKNLPIIVPGWEDSTLGNIFAAHCIEGDVKNVHTVRTGIEYMQF
                     LAEWYTQTAPKSPVGFFQIGGGIAGDFPICVVPMLHQDLRREGIPLWAYFCQISDSTT
                     SYGSYSGAVPNEKITWGKLAGDTPKFIIESDAAIVAPLVFAMVLGW"
     gene            5831..6733
                     /locus_tag="PLB01_13510"
     CDS             5831..6733
                     /locus_tag="PLB01_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQQ78366.1"
                     /translation="MVRKLVLAAGLVAALGCGRSEAPKPAPPAAEKPYPLKGVVVSVD
                     AGAGRVIVRHEEIRGYMDAMTMPFNVAEPKMLAELKPGDEIEAKLVVGEKASRLEGIV
                     VSRHGAPVSTQTIPEQGPYGKPGDAAPATVLTGQDGKPLSLADFRGKAVALTFIFTRC
                     PLPEFCPRMNAQFAEMETLLAKEPALYAKTQLLSVSFDPEHDTPEVLREYRERFLRGV
                     KGVAPAHWKFATGTPDRIKEFAGFFGLTYLKSGEQFVHSLATAVIRPDGKVFSIRRGN
                     DWEPPAVIEDLRKAVAGEAPKAAP"
     gene            6730..7305
                     /locus_tag="PLB01_13515"
     CDS             6730..7305
                     /locus_tag="PLB01_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQQ78367.1"
                     /translation="MIRPLVLAAALLFAGGAAAQPATRKDGGGEKAPDFTLVTQDEKV
                     FTLGKDGRGRPILLTFIFTSCPGACPLVVDECLEAARAAGKGKPAKDRPLVVALSFDP
                     DVDTPKRLREHMKENKFKRSEIVFLTGGRGAVDRAVRDWEVNVGRDAKGDIFHGFQTA
                     VIDGNGTIVARYYGAGIDVKALTADVRAAAR"
     gene            7331..>7765
                     /locus_tag="PLB01_13520"
     CDS             7331..>7765
                     /locus_tag="PLB01_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ78368.1"
                     /translation="MSILEAQGLTKTYRLGEERVVGVVDASLTLEKQSFAVLAGPSGS
                     GKTTLLNLLGLLDSPDGGSLRFEGADVSTLDERGRARLRRERLGFVFQAHQLVPVLSA
                     EENVALALWIRKLPEETCRSRARAALASVGLGGLEGRRPDALS"
BASE COUNT         1198 a         2654 c         2749 g         1164 t
ORIGIN      
        1 ccagtcgccg aacgaggcct cgagcgcggc cttgatctcg gccgccggca ggctgctcgc
       61 gacgaggagc gtcgcggcgt cggggcgcac gagggccgca tgggctgcct tcgcctcgtc
      121 gagcgtgagg gcgccgacgg acgccggcgt gccgcctgcc ggccaggcgt acgggtgcgc
      181 gtcgccgaag agggcgcgcg cggccacccg gtcggccacg acgggcgcct cgtcctcgct
      241 ctgtgagatc tcgtcgagcg tcagacgttt gacgcgctcg aagtccgccg gcgaaaagcg
      301 cggccgccgc acggcgtcgg cgaggagcgg gacgccctgc ccgacgttgc gcgagaggat
      361 cgtgaggctt gcctggatcg attgccgcga ggcccccgtg gagaagctgc cgccgaggct
      421 cgcgaccgct tcgccgaagg ccagcgcgtc gcgcgtcccg gcgccctcgt ccagcatgcc
      481 ggcggagagg gacgcgaggc ccgcgcgcgc ggcgctcgcg tcgagcgctc cctcgggccg
      541 gaacacggcc gcgatgcgcg tgagcggcag gtcgggcgtc ttccagtact gcacggcgag
      601 cccgttcgcg agcttgaagc gctccgggga agtcgggcgg aaggcggcgt ccgggccgtc
      661 ggcgggccgc gcgtcgcgcg ccgagggcgc gcgcttcggc tgctcgggga ggacgcggta
      721 gacgacgcgg gcgtccgccg tgaggacctg cttcgcggtc gcaaggacgg aaaccggcgt
      781 cgcgttcctg tagcggtcga ggtcgcgctt gaagccgttg gggtcgcccc aggcgtcctc
      841 gtagaagttc aggaggtcgg cgcgcgtctc gaggcgctgg agagccgaga gcttcgcgag
      901 ctcggtggtc tccttccgct gggccagctc gcccggcttg atcccctcgg cgaggagccg
      961 cgcgatttcc tcgtcgacgg ccttctcgat tctcccgagg tccgcgtcgg cgcgcgcctg
     1021 gacgtcgatc cggaacaccg agccgagggc ggcgctcgcg ttcgaggccg agacggatgc
     1081 cgcgagcttg tcgtcgatca cgaggcgctt gtagagccgg ctgctcttgc cgtccgaaag
     1141 aacggaggcc aggaggtcgc aatcggcgtc gcccggggtc agcgcgggcg gcgcgtggta
     1201 cgcgaaggcg acgagcggga gctgcacctt gtcgtacatc gcgccgcgcc tgacgccgtc
     1261 gagcttcgcg ggcggcacgg gcttgcgcgg cggctcgccg ccgcgcggga tgcccccgaa
     1321 gagcttctcg acgagcggct tgatcgccgc cgggtcgaag tctcccgcca cgacgaggct
     1381 cgcgttgttg gggacgtagt acgtcgcgaa gaaatccttc acgtcggccg cgacggcggc
     1441 cttcaggtcc tccgcggtgc cgatcgtggg gaagtggtag gggtgcgacg ggggatagag
     1501 catctgctgg atcgcgagct cggcgcgccc gtagggctgg ttctcgtagc tctggcgcag
     1561 ctcgttgagg acgacgtcgc gctggaggtc gaccttcttc tggtccatcg cgcggggaag
     1621 gccctcgagg cgttcggcgt cgagccagag gagcgtcggg aggagcgacg ccggaccgga
     1681 ggagtagtag ttcgtccggt cggaggtcgt cgaggcgttg ttcgagccgc ccgacgcctc
     1741 catgagccgg tcgaactcgc cgttcgggac gcgctcggtc cccatgaaca tgaggtgctc
     1801 gaagaggtgc gcgaaaccgc tgcggcgggc cggctcgtcc ttgctcccga cccggtacca
     1861 gatgttgatc gccgcgacgg gcagcgtgtg atccgggtag aggatcaccg tcattccgtt
     1921 cgcgagcttg tatttctcga cggcgaagct ctgggctgcg gtgggggagg agaaggccag
     1981 agagacgatc agcgcgagga tggcggctgc ggtgggggca cgcttcatgg aaagagaata
     2041 cgggggattt cttagaatgg ttatagggcc ggttgccgag ccgcgtctcg cgagggatgg
     2101 ccgcgcctgc cgcgccgccg tccccctctt caccttctaa gaaatcttct cttctcttcg
     2161 gtccctcttc ccctggcatt cgtcacaaag ccctttgacc aggatctcga ccgcgtcgtc
     2221 ctttagcgcg cgcgggacgc gcacgccgcg gcggagcgaa accttgagcg tgtcgaggca
     2281 ggcgagggag ccgcaggacg tgcagaggaa gtgcgggtgg tccttgcccc cggggcgcgc
     2341 gcccgccgcc tcgaaccgcc agacgtggtc gccgaggtcc ctgcgccggg cgagtccggc
     2401 cttcacgagc tcgacgaggt tccggtacac ggtcgcccgg tccgacacgg cgccttcgag
     2461 cgccgaggca aggtcggcgt ggctcgcagg cgcggtcctt gcacgtagtg ccgcgaggac
     2521 cgcgaggcgc gccggagtga cgcgcaagcc cgcgcgcctg aggtcggcga tttggttggc
     2581 gccggtcgga gttttcgggg cgcgagtccg cacgcggcga gcctaacagg ggcagaatga
     2641 aacacgggag gacccatgac tgacgaaaag aacgaccgca gggacttcgt gaagacggcc
     2701 gccgtggccg cggcaggcct cgcgatcaag gacgcggccg cgccctcggt cgcgaagggc
     2761 gcgacgccga tcacgctccc ggcactgcct tgggccgaca ccgccctcga gcccttcatc
     2821 tcgaagaaca cgatcggctt ccactacggc aagcaccaca aagcctacgt ggacaacctc
     2881 aacaacctcg tggccggcaa gcccgaggcc gacctgcccc tcgaggcgat cgtgaagggc
     2941 gccgcgggca aggccgacca ggccgccgtc ttcaacaacg cggcgcagat ctggaaccac
     3001 acgttctact ggaacagcat gcgccccaag agcgacgcga aggtcccgaa gcagatcgcg
     3061 gacctgatcg gggcctcgtt cgggagccac gaggagttcg tcaagcagtt ctcggccgcc
     3121 gcgatggggc agttcgggag cggctgggcg tggctcgtcg ccgagggcgg caaactgaag
     3181 gtcgtcaaga cgccgaacgc ggagacgccg ctcacgacgt ccgcgacgcc gctcctgacg
     3241 ctcgacgtct gggagcacgc gtactacctc gactaccaga accgccggaa ggactacgtc
     3301 gccgcggtcg tcgacaacct cctgaactgg gacttcgcgc tgaagaacct gccgaaggcg
     3361 tgacttcggt gaacgctgac ctcgaggcgc gtcaggccgt ggtcgacacg ctcaccgagc
     3421 tcttcctcgc gacggaccgt cgcgactggc ccgcggtcga aggctgtttc gccgagaccg
     3481 tcacgttcga catgacgtcg ctggcgggcg gccagcccgc ccgactcacg ccccgggaca
     3541 tttcgtcggc gtggacgcaa ggcctcgcgc ccatcgagtc ggtgcatcac caggcgggaa
     3601 acttccgcgt ccggatcgac gggaacgaag cttcggcgtt ctgctacgcc atcgcgtatc
     3661 actaccggcg ggtcgtcagc ggccgtaacc ttcggacgtt cgtcgggagc tacgacttcc
     3721 atcttgcccg cgacggagcg cagcggtggc gcatcgacct tttccgcttc aacatcaaat
     3781 tcctcgacgg caacctcgac ctgcattcgg aggacgccgc cggctgatgc tcaggccctt
     3841 tcgctctcga ggcggcggat gtaccagtcg acgccgtcct cgagggcgac cggcttgcgc
     3901 gcgaagccct gctttctcag gcggtccatc ttcgcctccg tgaagtactg gtagcgctcc
     3961 cggatgccca cgggcgtgtc gacgaattcg atgcgctccg ggacggcgag agaggcgaag
     4021 acggccttcg cgaggtcgag gaacgtgcgc gccttgccgg tccccacgtt gtagatcccg
     4081 gcggtgatcg gccgcttcag ggcgaaccac atcgcctcga ccgtgtccga gacgtccacg
     4141 aaatcgcgcg actggtgccc gtgggcgacg ccgtcccggt gggaccggaa gaggcggatc
     4201 tcgcccttcg ccttcacctg gtcgtacccg tggaggacga cgctcgcctg cgggcccttg
     4261 tgccgctcgc caaacccgta gacgttgaag aagcggaagc cggcccaagt cggcggcgtg
     4321 cggccggccg cggcctcctc gagagcccac aggtcgaacc gtcgcttgga ctcgccgtac
     4381 gggttcaggg gcttcaggct cgggacgagc gcgtcgtcgt cgtcgaagcc gagggcgccg
     4441 tcgccgtaga cggccgcgct cgacgcgtag acgaacggga tcccgagccg ccgggcctcg
     4501 gtccagaggt gtttcgagta gtcgacgttc aggcgcttga ggagcccctc gtcgagctcg
     4561 gtggtggccg agatcgcgcc catgtggacg atgccggcga gggcgggacg tccgcccgcg
     4621 agccagccgg gcagctcctc gatgtcgagg atcttcccga agtcgaggcg gcggtgctcc
     4681 gggcgcatcg tgaaggcctc gcggcggtcc acgctgacga cggggacgcc gcgggcgttg
     4741 caggagtcga cgaagcgggc gccgatgaac ccggcggccc cggtgacgag aagcgtgtcg
     4801 gtgctcaccc cccgatccta agggatgccg cttgtcgtct tcacgcgttc ttcctatgct
     4861 ccggccctat gacgatcacc tcgttcgtga agcaccattt ccgccacttc aacgccgcga
     4921 cggtcgtgga cgcggccgag gcctggaacg gcctcctcgc gggcggcggc aagatgttcc
     4981 tgaccatggc gggggccatg tccaccgccg agatcggcct ctcgctggcc gagatgatcc
     5041 ggcaggacaa ggtccacgcg atcacgtgca cgggggcgaa cctcgaggag gacgtcttca
     5101 acctcgtcgc gcacgaccac tacgtccgga tcccgcacta ccgtcagctc agcccggccg
     5161 aggaggaggc cctccacgcg cggcacctca accgcgtgac ggacacctgc attcccgagg
     5221 aggaggcgat ccgccgcatc gagaaggccg tcctcgagga gtggcaggcc gccgaccgcg
     5281 cgggggagcg ccggttcccg cacgagttcc tctatcgcat ccttctctcg gggaagctga
     5341 aggagtccta ccagatcgac cccaaggact cgtggctcct cgcggccgcg cagaagaacc
     5401 tcccgatcat cgtcccgggg tgggaggact cgacgctcgg caacatcttc gcggcgcact
     5461 gcatcgaggg cgacgtcaag aacgtccaca cggtgaggac gggcatcgag tacatgcagt
     5521 tcctcgcgga gtggtacacg cagaccgccc cgaagagccc ggtcggcttc ttccagatcg
     5581 gcggcggcat cgcgggcgac ttcccgatct gcgtcgtccc gatgcttcac caggacctcc
     5641 gccgcgaggg catcccgctc tgggcgtact tctgccagat cagcgactcg acgacgagct
     5701 acggcagcta ttccggcgcg gtcccgaacg agaagatcac gtggggcaag ctcgcgggcg
     5761 acaccccgaa gttcatcatc gagtcggacg ccgcgatcgt ggcgcccctc gtcttcgcga
     5821 tggtgctcgg atggtgagaa agctcgtcct cgccgccggc ctcgtggccg ccctcggctg
     5881 cggcaggtcc gaggccccga agcccgcgcc gcccgccgcc gaaaagccgt acccgctcaa
     5941 gggcgtcgtc gtctccgtgg acgccggtgc aggccgcgtg atcgtccggc acgaggagat
     6001 ccgcggctac atggacgcga tgacgatgcc gttcaacgtc gccgagccga agatgctcgc
     6061 cgagctcaag ccgggcgacg agatcgaggc gaagctcgtc gtgggcgaga aggcttcccg
     6121 tctcgaaggc atcgtcgtgt cgcgccacgg cgcgccggtg tccacgcaga cgattcccga
     6181 gcagggcccg tacggcaagc ccggcgacgc cgcgcccgcg acggtcctca ccgggcagga
     6241 cggcaagccg ctctccctcg cggacttcag gggcaaggcc gtcgcgctca cgttcatctt
     6301 cacgcgctgc ccgctgcctg agttctgccc gcgcatgaac gcgcagtttg cggagatgga
     6361 gacgttgctc gcgaaagagc ccgcgctcta cgcgaagacc cagctcctct cggtgagctt
     6421 cgaccccgag cacgacacgc cagaggtgct ccgggagtac cgcgaacgct tcctcagggg
     6481 cgtcaagggc gtcgcgcccg cccactggaa gttcgcgacc gggacgcccg accgcatcaa
     6541 ggagttcgcg ggtttcttcg ggctcacgta cctcaagtcc ggcgagcagt tcgtccacag
     6601 cctcgcgacg gcggtgatca ggccggacgg gaaggtgttc tccatccggc gggggaacga
     6661 ctgggagccg ccggccgtga tcgaagacct ccgcaaggcc gtcgcgggcg aggcgccgaa
     6721 ggccgcgccg tgatccggcc tctcgtcctc gcggccgccc tcctcttcgc cggaggcgct
     6781 gcggcgcagc ccgcgacccg aaaggacggg ggaggcgaga aggcgccgga cttcacgctc
     6841 gtcacgcagg acgagaaggt cttcacgctc ggcaaggacg ggcgcggccg gccgatcctc
     6901 ctcacgttca tcttcacgag ctgtcccggc gcttgcccgc tcgtcgtcga cgagtgtctc
     6961 gaggccgcgc gggcggcggg gaaggggaag cccgcgaagg accgcccgct cgtcgtggcc
     7021 ctgtcgttcg acccggacgt ggacacgccg aagcgcctgc gcgagcacat gaaggagaac
     7081 aagttcaagc gctcggagat cgtcttcctg accggcggcc gaggcgccgt ggaccgggcc
     7141 gtccgcgact gggaagtgaa cgtcggccgc gacgcgaagg gcgacatctt ccacggcttc
     7201 cagacggccg tcatcgacgg gaacgggacg atcgtcgcga ggtactacgg ggccggaatc
     7261 gacgtgaagg cgctcaccgc cgacgtgcgg gccgcggccc gctgacacgg ccgttcccta
     7321 tactccgccc gtgtccatcc tcgaggccca agggctcacg aagacctacc gcctcggcga
     7381 ggaacgggtc gtcggcgtcg tcgacgcctc gctgacgctc gagaagcagt cctttgccgt
     7441 gcttgccggc ccttcgggca gcggcaagac gactctcctg aacctcctcg gacttctcga
     7501 cagccccgac gggggatcgc tgcgcttcga gggggcggac gtctcgacgc tggacgagcg
     7561 cggccgcgcg cgcctgaggc gcgagcgcct cggattcgtc ttccaggcgc accagctcgt
     7621 gcccgtcctg tcggccgaag agaacgtcgc gctcgcgctc tggatccgca agctccccga
     7681 ggagacgtgc cgctcgcggg cgcgggctgc gctcgcctcc gtgggcctcg gcggtctcga
     7741 ggggcgccgc cccgacgccc tctcg
//
LOCUS       DAOMFY010000182         7740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2962_length_7740_cov_7.698504, whole genome shotgun sequence.
ACCESSION   DAOMFY010000182 DAOMFY010000000
VERSION     DAOMFY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2962_length_7740_cov_7.698504"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLB01_13525"
     CDS             complement(<1..362)
                     /locus_tag="PLB01_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78369.1"
                     /translation="MLSDAVTVFREDAEDAVRIVSGMLRRGEGGEAFRERRRTIAWSA
                     TEAGLQSPSIVEESGTAVRVTRGKDSLLVACDGDGPDALREVVREAARRAGGAPFFKT
                     KAPRERPESRGPSGPDEEG"
     gene            435..1823
                     /locus_tag="PLB01_13530"
     CDS             435..1823
                     /locus_tag="PLB01_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78370.1"
                     /translation="MKVVFGFLLVLVRVGGLAGPPAATLSVRVAPALDPQGAAWIVQL
                     FEESPEGRKPVDERPVDAGGMWTSTAARAGARYCLRVSTAAGAGWFADEKSFEWSGSE
                     PSGRPREIRLSSSAFRARLVLGKKPLSARVTFTDREGLVSVPFAAKTDGTFEGVLPRD
                     GWWSVTVAADRPHFKTTFDVKVDPVFRDGTPPDVEIRVVSKAIQGEIVDLRNVRVPVA
                     SLSVMGNGNSDSMKVEGGSFKWARVVDGWMSVFGSASGRGSFDQSPIRRIRVSGGWVD
                     PGFIALEIRPTYVLKGRAVLGSGEPAANARIRLLSSTFNENLRTPADGRFQMTVRGGG
                     ESACLLVVDPSKAARLVPVPASESEQDVPVAASPGTLSLEFTPRGERRFLELAANGCN
                     LPSFFFREITGAKSAVSGMTSTFSFRASAGSYSLCGLEYGDLEDDAEIPPEACVSGVL
                     PPGGTLSLRLNP"
     gene            1864..2142
                     /locus_tag="PLB01_13535"
     CDS             1864..2142
                     /locus_tag="PLB01_13535"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HQQ78371.1"
                     /translation="MALSDARLAAQAAYVARRILSEGLVKTAAAAELRRALETALVED
                     RDRERALDDDVKKILAQNAATIRGAGVDYAEMFRKAKKMLAAQKKIPL"
     gene            2153..3715
                     /locus_tag="PLB01_13540"
     CDS             2153..3715
                     /locus_tag="PLB01_13540"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HQQ78372.1"
                     /translation="MTRSIDERLATLEALREKSRLGGGVERREKQHKAGKLTARERVE
                     LLGDPGSFDEIDPFVVHRARDFGMDDPANEIPGDGVVAGSVRVEGRPVFVFAQDFTVF
                     GGSLSETNAAKICKLMDLAMKVGAPLVGLNDSGGARIQEGVVSLGGYADIFLRNTLAS
                     GVVPQISAILGPCAGGAVYSPAITDFVLMARGTSSMFVTGPDVIKTVTHEDVTKEALG
                     GADTHASVSGVAHVVADSDADCLSRIRTLLGFFPSNNQEDPPRRENGDPVDREDPELD
                     RVVPAAPNQPYDMIGLIKRIVDDGELFEIHADYAKNIVVGFARLGGKPVGIVANQPDH
                     LAGVLDIDASVKGARFVRFCDAFNIPLVTFEDVPGFLPGTKQEFGGIIRHGAKLLYAF
                     AEATVPKLTVITRKAYGGAYCVMSSKHIRTDFNYAWPTAEIAVMGAEGAVNILYHREL
                     AASKHPSEARAEKIAEYEEAFANPYVAARRGYVDDVIAPRTTRPRLIRALDLLGNKRE
                     VMPQKKHGNIPL"
     gene            3712..5202
                     /locus_tag="PLB01_13545"
     CDS             3712..5202
                     /locus_tag="PLB01_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQQ78373.1"
                     /translation="MSAPRLLIANRGEIAVRIIRACRDLGVSPVAVYSEADRGSLHVR
                     LADHAVAIGPPPARESYLVPEKLIAAARKTGATLVHPGFGFLSENAAFAQAVIDAGLT
                     WVGPRPFSIAAMGSKTESRQRMLAAGVPVVPGMTTPAASAAEVAAFAKENGYPLLLKA
                     AAGGGGKGMRVVRAESEVAEAFARTQSEAKSYFADDRVYAELFVEHPRHIEVQILGDA
                     TGKVLAIGERECSLQRRHQKVLEECPSAAVSPELRAKMEAVAVAAGKAVDYVSAGTVE
                     FLLAKDGRFFFLEMNTRIQVEHPVTEMVYGVDLVAEMIRIALGRPMSFAEKPALRGHA
                     IECRVYAEDPGRAFAPSPGRITALRWPGGPGIRVDAGLTAGDVVPLDYDPMVAKITAW
                     GVDRDEARRRMLRALAETRIQGIQTSVPFFVSLLNDPRVEKNDISTQFLDTYKYEPPV
                     AEEGLRDLALSAAVLEALEQTKSVRPQTKGAVPAPWRTVRPTHGVR"
     gene            5202..5729
                     /locus_tag="PLB01_13550"
     CDS             5202..5729
                     /locus_tag="PLB01_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQQ78374.1"
                     /translation="MKYHARFPGAAPGGVALRVEAPSPSYEIETEGRVLAGNARRLGD
                     GVWSLVTAEGRQAEVRIERTPDGRMRVRIGAVTFEFDLLDELTARALLATGGRAVKRA
                     KHVAAAMPGRVLRVLVAAGDTVTAGQPLVVLEAMKMENEVKSPQDGVISGIAVTAGQP
                     VNAGEVLVRFAADPA"
     gene            5779..6960
                     /locus_tag="PLB01_13555"
     CDS             5779..6960
                     /locus_tag="PLB01_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQQ78375.1"
                     /translation="MKTLARTAAALLFVLGLSCHAAQTAPPPAAAKPAAAKTDPLTEW
                     VEGLFAWGAGQTTVEEITTVKIPNTRIYLAKRTYAQDPRMNDQAHLFVEDGGKVLVGD
                     IFYDEDRAKAPAPVRTDADLASMRGKVRQFLIGGFNLVLDPATDRKNWKGVTVRTDTG
                     YGSTPMSAFIKADDGTLLVLGRFWDRNRSIAEQRREGIRLAGTPMTGPADAKVTVVEY
                     SDMQCGFCRKRTADFDALVSKLSKELKIKRYVKSFPLTSAHPWAFRAASAGRCFFDLQ
                     GAESFFRWKAQVYAKQDELSVPGVDAFAMDYAYANEIPDAQFRSCYLQSKSNDKIHAD
                     LTEGWALRVRSTPTYYIDGVYVSWFADNLMEEFLRTTYLGGKGLPLPASSPAAAGTAK
                     H"
     gene            complement(7013..7579)
                     /locus_tag="PLB01_13560"
     CDS             complement(7013..7579)
                     /locus_tag="PLB01_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQQ78376.1"
                     /translation="MQKKLDEELRKQEARAAKQQDSAAKKKQAEIEQVSEEARKARET
                     EDAARAARDRADREEAARLAREGAAKRREEAQAAAAAAPTAPAVKEGDVVDIEACDTP
                     PVSTRQVSPAVPLLAKQRRVSGTVLLRVLVDETGKPAKIEILRDVTPSVGLGASSVQA
                     LEQWRWKPATKNGVRVKTWMAVQVPFRN"
BASE COUNT         1148 a         2725 c         2685 g         1182 t
ORIGIN      
        1 ccctcctcgt cggggccgga cgggccgcgg ctctccggcc gctcccgcgg ggccttcgtc
       61 ttgaagaacg gcgcgccgcc cgcgcggcgg gccgcctcgc gcacgacctc ccgcagcgcg
      121 tcgggaccgt ccccgtcgca ggcgacgagg agggagtcct tcccgcgcgt cacgcggacg
      181 gcggtcccgc tctcctcgac gatcgacggc gactggagcc cggcttccgt cgcgctccac
      241 gcgatcgtgc ggcgacgctc gcggaacgcc tcgcccccct cgccgcggcg gagcatcccg
      301 gagacgatgc ggacggcgtc ctccgcgtcc tcgcggaaga ccgtgacggc gtcgctcaac
      361 acgggggatt gtagaagcgc cccgcgatcc gacgacgggg gtccggcgag aggcgtatat
      421 agggaagaag agccatgaag gttgttttcg ggtttctcct cgttctggtg cgagtcggcg
      481 gcctggccgg gccgccggcc gcgacgctgt ccgtgcgcgt cgctccggcc ctcgatccgc
      541 agggtgccgc ctggatcgtg cagcttttcg aagaatctcc tgaaggtcgg aaacccgtcg
      601 acgagcgccc cgtcgacgcg ggcgggatgt ggacgtcgac cgcggcgcgg gcgggcgcac
      661 ggtactgcct ccgcgtttcg acggcagccg gtgcgggatg gttcgcggac gagaaatcct
      721 tcgaatggtc cggaagcgaa ccgagcggcc ggccccgcga aatccgcctt tcgtcgagcg
      781 cgtttcgcgc gaggctcgtc ctcgggaaga agcccctctc ggcgcgcgtg acgttcacgg
      841 accgcgaggg cctcgtctcg gtgccgttcg ccgcgaagac ggacgggacg ttcgaaggcg
      901 tcctcccgcg cgacggctgg tggagcgtga cggtcgccgc ggaccgcccg cacttcaaga
      961 cgacgttcga cgtgaaggtg gacccggtct tccgcgacgg cacaccgccg gacgtcgaga
     1021 tccgtgtcgt gagcaaggcc atccagggcg agatcgtgga cctcaggaac gtgcgcgttc
     1081 ctgtcgcatc gctttccgtc atgggaaacg ggaattcgga ttccatgaag gtggagggag
     1141 gttcgttcaa atgggcgcgc gtcgtcgacg ggtggatgtc cgtctttggc tcggcctcgg
     1201 gccggggctc gtttgaccag tctcccatcc gaaggatccg cgtctccggc ggctgggtcg
     1261 atcccgggtt catcgcgctc gagatccggc cgacgtatgt gctgaagggg cgcgccgtcc
     1321 ttgggtcggg cgagccggcc gcgaatgcgc gcatccgcct gctctcttcc accttcaatg
     1381 aaaacctccg aactccggcc gacggccgct tccagatgac ggtccggggc ggcggcgaga
     1441 gcgcgtgtct ccttgtcgtg gacccctcga aggccgcccg gctcgtgccg gtcccggcct
     1501 cggagagcga gcaggacgtg ccggtcgccg cctcgccggg cacgctctcc ctggagttca
     1561 cgcctcgcgg agagcgccgg ttcctggagc ttgccgcgaa cggctgcaac ctgccgtcgt
     1621 tcttcttccg cgagatcacg ggggcgaaga gcgcggtctc cgggatgacg tccactttct
     1681 cgttccgagc ttccgcgggc tcttattccc tttgcgggct cgagtacggc gatctcgagg
     1741 acgacgccga gatcccaccg gaggcctgcg tctcgggcgt cctcccgccc ggcggcaccc
     1801 tctcgttgcg actcaatccg taaagcgcga cggtttcgag ggtgggatat cctcgaacgt
     1861 cctatggcct tgtcagacgc ccggctggcc gcgcaggccg cgtacgtggc gcgccgcatc
     1921 ctttccgagg ggctcgtgaa gacggccgcg gccgcggaac tgcggcgggc gctcgagacc
     1981 gccctcgtgg aagaccgcga ccgcgagcgg gccctggacg acgacgtgaa gaagatcctc
     2041 gcgcagaacg cggcgacgat ccgcggcgcg ggagtggact acgcggagat gttccggaag
     2101 gcgaagaaga tgctcgccgc ccagaagaag atcccgctgt aaagggagac ccatgacgcg
     2161 gtcgatcgac gaacggctcg cgacgctcga agccctgagg gagaagtcgc ggctcggggg
     2221 cggcgtggag cgccgcgaaa agcagcacaa ggccggaaag ctcacggcgc gcgagcgcgt
     2281 cgagctcctc ggggatcccg gctccttcga cgagatcgac ccgttcgtcg tccaccgcgc
     2341 ccgcgacttc gggatggacg accccgcgaa cgagatcccg ggcgacggcg tcgtcgcggg
     2401 ctccgtccgc gtggagggcc ggcccgtctt cgtcttcgca caggatttca cggtcttcgg
     2461 cgggtcgctg tccgagacga acgccgcgaa gatctgcaag ctgatggacc tcgcgatgaa
     2521 ggtcggggcg ccgctcgtcg gcctcaacga ctcgggcggc gcgcgcatcc aggagggcgt
     2581 cgtctccctc ggcggctacg cggacatctt cctgcgcaac acgctggcct cgggcgtcgt
     2641 cccccagatc agcgcgattc tcggtccgtg cgcgggcggc gccgtctact cgcccgcgat
     2701 cacggacttc gtcctcatgg cgcgcgggac gagctcgatg ttcgtgaccg gacccgacgt
     2761 catcaagacc gtcacgcacg aggacgtcac gaaggaggcg ctcggcggcg ccgacacgca
     2821 cgcctccgtc tccggagtcg cgcacgtcgt cgcggactcc gacgcggact gtctgagccg
     2881 gatccggacg ctcctcggat tcttcccctc gaacaaccag gaggacccgc cgaggcgcga
     2941 gaacggcgat ccggtggacc gcgaggatcc cgagctcgac cgcgtcgtgc ccgcggctcc
     3001 gaaccagccg tacgacatga tcgggctcat caagcggatc gtggacgacg gggagctctt
     3061 cgagatccac gcggactacg cgaagaacat cgtcgtcggg ttcgcgcgcc tcggcggcaa
     3121 gccggtcggg atcgtcgcga accagccgga ccacctcgcg ggcgtcctcg acatcgacgc
     3181 ttccgtgaag ggcgcccggt tcgtgcggtt ctgcgacgcc ttcaacatcc cgctcgtcac
     3241 gttcgaggac gtcccgggct tcctgccggg cacgaagcag gagttcggcg ggatcatccg
     3301 ccacggcgcc aagctcctct acgcgttcgc cgaggccacc gtcccgaagc tcacggtcat
     3361 cacgcgaaag gcttacggcg gcgcctactg cgtcatgtcg tccaagcaca tccggacgga
     3421 cttcaactac gcgtggccga cggccgagat cgccgtcatg ggcgccgagg gtgccgtcaa
     3481 catcctctac caccgcgagc tcgcggcctc gaagcatccc tccgaggcgc gggcggagaa
     3541 gatcgccgag tacgaggagg cgttcgcgaa cccctacgtc gcggcgcggc gcggctacgt
     3601 cgacgacgtg atcgcgcccc ggacgacgcg gccgcgcctc atccgcgcgc tggaccttct
     3661 tgggaacaag cgcgaagtca tgccgcagaa gaaacacggg aacatcccgc tgtgagcgcg
     3721 ccgcggcttc tcatcgccaa ccgcggagag atcgccgtcc ggatcatccg cgcctgccgc
     3781 gacctcgggg tctcgccggt ggccgtttat tccgaggccg accgcgggtc gctgcacgtc
     3841 cgcctcgcgg accacgccgt cgcgatcggc ccgccgcccg cccgtgagag ctatctcgtc
     3901 ccggaaaaac tgatcgcggc ggcccggaaa acgggcgcga cgctcgtcca cccggggttc
     3961 ggcttcctct ccgagaacgc cgcgttcgca caggccgtca tcgacgcggg tctcacgtgg
     4021 gtagggccgc ggccgttctc gatcgccgcg atgggcagca agaccgaatc ccgccagcgg
     4081 atgctcgcgg cgggcgtccc cgtcgtcccc ggcatgacga cgcccgcggc gtcggcggcg
     4141 gaggtcgcgg cgttcgcgaa ggagaacggc tacccgctcc tcctcaaggc ggcggcgggc
     4201 ggcggcggca agggcatgcg cgtcgtgcgc gccgagtccg aggtcgcgga ggcgttcgcg
     4261 cgcacgcaga gcgaggccaa gtcctacttc gccgacgacc gcgtctacgc cgagctcttc
     4321 gtcgagcatc cgcgccacat cgaggtccag atcctcggcg acgccacggg aaaggtcctc
     4381 gcgatcggcg agcgcgagtg ctcgctccag cgccgccacc agaaggtcct ggaggaatgt
     4441 ccgtcggcgg ccgtctcgcc cgagctgcgc gcgaagatgg aagccgtcgc ggtcgcggcc
     4501 gggaaggccg tcgactacgt ctcggcgggc accgtcgagt tcctcctcgc caaggacggg
     4561 cggttcttct tcctcgagat gaacacgcgc atccaggtcg agcaccccgt cacggagatg
     4621 gtctacggtg tggacctcgt cgccgagatg atccggatcg cgctcggccg gcccatgtcg
     4681 ttcgccgaaa agcccgcgct gcgcgggcac gcgatcgagt gccgcgtgta cgccgaagac
     4741 ccggggcgcg cgttcgcccc gtcgccgggc cgcatcacgg cgctgcgctg gcccggcggg
     4801 ccggggatcc gcgtggacgc cggcctcacg gcgggcgacg tcgtcccgct cgactacgac
     4861 ccgatggtcg cgaagatcac ggcctggggc gtggaccgcg acgaggcgcg ccgccggatg
     4921 ctccgcgcgc tcgcggagac gcgcatccag ggcatccaga cgtccgtgcc gttcttcgtc
     4981 tccctcctga acgacccgcg cgtcgagaag aacgacatct cgacccagtt tctcgacacc
     5041 tacaagtacg agccgcccgt cgcggaagag ggcctgcggg acctcgcgct ctcggcggcc
     5101 gtcctcgagg cgctcgagca gacgaagagc gtccggccgc agacgaaggg cgcggttccg
     5161 gcaccgtggc gaacggtccg gccgacccac ggggtgcgtt gatgaaatac cacgcgagat
     5221 tcccaggcgc cgctccgggc ggcgtcgccc tccgcgtgga ggccccgtct ccgtcctacg
     5281 agatcgagac cgagggccgg gtcctcgcgg gcaacgcgcg ccggctcgga gacggcgtgt
     5341 ggtccctcgt gaccgcggag ggccgacagg ccgaggtccg gatcgagcgg acgcccgacg
     5401 gcaggatgcg cgtgcgcatc ggcgccgtca cgttcgagtt cgacctcctc gacgagctca
     5461 cggcgcgggc cctcctcgcg accgggggcc gagccgtgaa gagggccaag cacgtggcgg
     5521 ccgcgatgcc gggccgcgtg ctgcgcgtcc tcgtcgcggc gggagacacg gtcacggcgg
     5581 ggcagccgct cgtcgtcctc gaggcgatga agatggaaaa cgaggtcaag tcgcctcagg
     5641 acggcgtcat ctccgggatc gccgtcacgg cggggcagcc cgtcaacgcg ggcgaagtgc
     5701 tcgtccggtt cgcggcggac ccggcctgac ggcccgggga ggtttccggg ggcggggaga
     5761 tcgcttaatc tccgccccat gaagacgctc gcccgcaccg ccgccgcgct cctcttcgtc
     5821 ctcggcctct cgtgccacgc cgcccagacc gcgccgccgc cggccgccgc caagccggcc
     5881 gccgcgaaga ccgatccgct cacggagtgg gtcgaggggc tcttcgcctg gggcgccggc
     5941 cagaccacgg tcgaggagat cacgacggtc aagatcccga acacgaggat ctacctcgcg
     6001 aagcggacgt acgcgcagga cccgcgcatg aacgaccagg cgcacctctt cgtggaagac
     6061 ggcggcaagg tgctcgtcgg cgacatcttc tacgacgagg accgcgcgaa ggcacccgcg
     6121 cccgttcgca ccgacgcgga tctcgcgagc atgcgcggga aggtgagaca gttcctgatc
     6181 ggcggcttca acctcgtcct cgatcccgcg accgaccgca agaactggaa gggcgtgacg
     6241 gtgcggacgg acacgggcta cgggtccacc ccgatgagcg ccttcatcaa ggcggacgac
     6301 gggacgctgc tcgtcctcgg ccggttctgg gaccgcaacc gctcgatcgc cgagcagcgg
     6361 cgggagggaa tcaggctcgc cggcactccg atgacgggcc ctgcggacgc gaaggtcacg
     6421 gtcgtcgagt actcggacat gcagtgcggc ttctgcagga agcgcacggc ggacttcgac
     6481 gccctcgttt cgaagctttc caaggagctg aagatcaagc gctacgtcaa gtcgttcccg
     6541 ctcacgtccg cccacccctg ggcgttccgg gcggcgtcgg ccgggcgctg cttcttcgac
     6601 cttcagggcg ccgagtcgtt cttccgctgg aaggcgcagg tctacgcgaa gcaggacgag
     6661 ctcagcgtgc cgggcgtgga cgcgttcgcg atggactacg cgtacgcgaa cgagatcccg
     6721 gacgcgcagt tccggtcgtg ctacctgcag tcgaagtcga acgacaagat ccacgcggac
     6781 ctgaccgagg gctgggccct gcgcgtccgc tcgacgccga cgtactacat cgacggcgtc
     6841 tacgtctcgt ggttcgcgga caacctcatg gaggagttcc tccggacgac gtacctcggc
     6901 gggaagggcc tccccttgcc cgcgagctca ccggctgcgg caggaacggc gaagcactag
     6961 aagcgcgcga gcgccccgcc gctcttcacc ttctaagaag tcttctcttc tcttaattcc
     7021 tgaacggcac ctggacggcc atccaggtct tcacccggac gccgttcttc gtcgcgggct
     7081 tccagcgcca ctgctcgagc gcctgaacgc tcgaggcgcc gagtccgacg ctcggcgtca
     7141 cgtctctcag gatctcgatc ttcgccggct tgccggtctc gtccacgagg acgcggagca
     7201 ggaccgtccc ggagacgcgc cgctgcttgg ccaagagcgg cacggccggg ctcacctggc
     7261 gcgtggacac ggggggcgtg tcgcaggcct cgatgtccac gacgtcgcct tccttcacgg
     7321 cgggggcggt gggcgcggcg gcggcggcag cctgggcctc ttcccgtctc ttcgcggctc
     7381 cctcgcgggc gaggcgtgcg gcctcctcgc ggtcggcgcg gtcgcgcgca gccctcgccg
     7441 cgtcctcggt ttcgcgagcc ttgcgggctt cttcggacac ctgctcgatt tcggcctgct
     7501 tcttcttcgc ggcggagtcc tgctgcttgg ccgcgcgcgc ttcctgcttc ctgagctcct
     7561 cgtcgagctt cttctgcacc gcggcctgga agagcgggtc gtccttgccg acgacgacgg
     7621 gcgcgggcgt cggaaccgaa aactacgtct ttggctgcca tgtctgtatt tctccgaaat
     7681 ctgtgcgggg gatgggctta cttctcgatc acggcgaaga ggtcttcctc gcgcatcacc
//
LOCUS       DAOMFY010000183         7724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2971_length_7724_cov_6.463033, whole genome shotgun sequence.
ACCESSION   DAOMFY010000183 DAOMFY010000000
VERSION     DAOMFY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_7724_cov_6.463033"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLB01_13565"
     CDS             <1..295
                     /locus_tag="PLB01_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78377.1"
                     /translation="APPAPAKPASPAPAAPAKHPQAPAERAPKIPPTSIPTLSKPMEE
                     LMSFAFTPNEAFIVSRINGMWDVRSIARISPFPEAEVLRVFAKLQSSGVISFK"
     gene            292..1998
                     /locus_tag="PLB01_13570"
     CDS             292..1998
                     /locus_tag="PLB01_13570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HQQ78378.1"
                     /translation="MSFADLRPAVSTLVALVLLLVVAVVIRQLARARHGAAELARKNR
                     ELATLREITAVVSSVAPGETVFAHVFDALKRAVPVRALAAVSFEEDPDGQAVVAFGGE
                     VTIERRAFLDWLSLQRELESPFLPSTPPPRIAQGPGRELVLNPRLGCQVFLPLQTPAL
                     IAGVLVAESDDTALVTPQVLQELCVFADHLALSLQDRNLRRQMQAVNERLQGRAEMLQ
                     RILELSNELKTHLTLEHLVQSIVQAVHHSLGYRTVALSLYEASENVFERRAQMGFDDA
                     LPGAPPRRVPRDEVARWFAERYRISKSYFVSHLDRQESMDDSGRIDRRRRGAAPGAWH
                     PNDLLFVPLTAGDQLVGVLQVDQPRSGLVPRLEDVQALEIFANQAVTALQSARAYETT
                     RQMSVRDSLTEAFNHRYFQETLSRELARHERSGQPLALVMLDIDDFKKINDRWGHPVG
                     DVVLRGLVEEMTKGVREMDTVARYGGEEFALIFPETTPAKAWVVADRLRKRVASRLFA
                     APHVEQALAVTISLGLSTYPEDGSTKRLLIERADQALYQAKRSGKNCLVTAASLADLK
                     AL"
     gene            1995..3416
                     /locus_tag="PLB01_13575"
     CDS             1995..3416
                     /locus_tag="PLB01_13575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQQ78379.1"
                     /translation="MIEDEYRGPRWWPLSALQGKAVAGIGPAWAGLTALCIGLAILEA
                     RLDWSGMPVRIGPVTVGVSVYPPLSISLVLAVWIGPLWGLVPAWLATFSSALTAGVPL
                     PSAAVFALATPAEVFLLWGSMVALNIDPALPRFSDLRRFLAAGLVAASGSSPAILIWN
                     ESRHLDLVAGQRLWQGWLLGDLLQIALVAAPLLHFLGPRANAFLEKRFDARPKVEFRY
                     RDGVLWTGLVSVTLAAVVALGLRMALVSLRIPETAVTARGEPLLPRLREMTLFAGLLL
                     GVVLVTSSTFGASLARLGDREKSSARRDPLTGCLNRRAFYEAFALEADRSARLAAALS
                     VLTIDVDRFKVLNDAHGHAFGDEILRQLARRLTALVRSHDLVVRWGGEEFVVLLPHTS
                     PGDAAALAERIRAGVESPEFRVDGRAVSLTCSVGVAGTQVFPASADDLVAQSDAACYE
                     AKRAGRNRTVSVEIAALKAPSAS"
     gene            complement(3373..3993)
                     /locus_tag="PLB01_13580"
     CDS             complement(3373..3993)
                     /locus_tag="PLB01_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HQQ78380.1"
                     /translation="MAPQKPLPKSFYRKDTTDVARALLGCRLTRRLPDGTTRSVILVE
                     TEAYLGVKDRAAHTWGGRRTPRVAPMWREGGHAYVYFVYGMHYCLNVVTQEQGVPEAV
                     LLRAGIPEEEKEKISLKVKRGGEASGPAKLCAYLGVTTALSGASLAGPEIVLSRGPRW
                     RFDVVSGPRVGVDYAGPAAAWPLRFAVAGCPAVTKPKALSAPRSRR"
     gene            4010..4951
                     /locus_tag="PLB01_13585"
     CDS             4010..4951
                     /locus_tag="PLB01_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ78381.1"
                     /translation="MMPETRSVLVTGGAGFIGSHLADAFLEAGSRVTILDDLSTGVLE
                     RVPARARFVEGDLRTFDFEALLRAEKVDTILHHAAQIDVRKSVEDPVFDAEVNVLGTI
                     RLARAALACGVTQVVFASSGGAIYGDPEGFAADERHPTNPVSPYGVAKLAGEKYLFGL
                     AHGSPLVVTALRYANVYGPRQDGRGEAGVVGIWMARLLENQPCVIYGDGLNSRDFVYV
                     GDVVAANRAASERRLGGVFNVGTGVETTVNRLWDEVARACGSSAKAAHEAAKPGEQRR
                     SVLDVAKGRAALGLGAPVSLSEGLRRTAAWFRERPKP"
     gene            complement(4890..6209)
                     /locus_tag="PLB01_13590"
     CDS             complement(4890..6209)
                     /locus_tag="PLB01_13590"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQQ78382.1"
                     /translation="MRGRYRAEAVHSDPNMDYRALLRNVEKTLEAIEGTGNVSLTILR
                     VAEGIVHNFRNELGILGGRLYLREDNVYILERGFGRSKRTPAGLAVPASYGPIVRAVE
                     DGIVLMDRSDPSVDAEFEQRLGVTRFAAVSFGEEKYLLSFDVHPRVREEDLLLALGII
                     RTVLDSKVRTGHLEGLLQDARRIQMSILPRHVPQVGDFDIFGISNPAELVGGDFYDLI
                     PLSESLGVAIADASGHGLLAALLVRDIYVGLRMGVSNDIKIARTIERLNRIFSKSRLT
                     TKFVSLFYGEFDTDGDVVYVNAGHPGPLHFHAATGTFSEFTSTGMVLGPMPDAVYRRR
                     AAHLEPCDIVLLYTDGVVEAHDAKGREFGVARLRKLVAGSRRESAREIAHRVTNAVKS
                     WRGGAAPEDDVTVVVIKRDPKGPGANRVRASGAPGTTPPSGGAPPKG"
     gene            6208..>7724
                     /locus_tag="PLB01_13595"
     CDS             6208..>7724
                     /locus_tag="PLB01_13595"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQQ78383.1"
                     /translation="MDTLTRRQLVAASVASGIAAAARPIAAAGGATSAPAGERPFALA
                     EATIADLQAGMASGKWTARSITQAYLARIDAVDAGGPALRSVLEKNPDALAIADALDR
                     ERKEKGPRGPLHGIPVLVKDNIDTADRMATTAGSLALVGAKPPADAFVVRRLREAGAV
                     LLGKTNLSEWANIRSSTSTSGWSGRGGLTRNPYALDRNPCGSSSGSGVAAAANLCAAA
                     VGTETDGSIVSPSSANGIVGVKPTVGLVSRTGIIPISHSQDTAGPMARTVRDAAILLG
                     ALAGADGEDAATGASGHRAEPDYTAFLDPAGLRGARIGVVRGLFGFLDRVDALMAEAL
                     AALKRGGAELVDPVELTSAAKIGEPEGSVLYYEFKAGLNAYLARLGPGAPVKSLADVI
                     AFNEKHSKEEMPYFAQETFLKAEAKGPLTSFEYLEALAKSRRLARTEGIDATMDRHRL
                     DALVAPTLGPACVTDLVNGDRWLGDASTAPAVAGYPHVTVPAGFVFGLPVGLSFFG"
BASE COUNT         1051 a         2884 c         2640 g         1149 t
ORIGIN      
        1 ggctcccccg gcgcctgcca agccggcatc gccggcgccc gccgcccccg cgaagcaccc
       61 gcaggctccc gccgagaggg cgccgaagat cccgcccacg tccatcccga ccctctccaa
      121 gccgatggag gagctcatgt cgttcgcgtt cacgccgaac gaggccttca tcgtctcgcg
      181 gatcaacggg atgtgggacg tgcgctccat cgcgcggatc tcgcccttcc cggaggccga
      241 ggtcctgcgc gtcttcgcga agctccagtc gagcggcgtc atttcgttca aatgagcttc
      301 gccgacctcc gccccgccgt ctcgacgctc gtcgcgctcg tcctgctcct cgtggtcgcc
      361 gtcgtcatcc ggcagctcgc gcgggcccgg cacggcgccg ccgagctcgc gcggaagaac
      421 cgcgagctcg ccacgctgcg cgagatcacg gcggtcgtct cgagcgtcgc tcccggcgag
      481 accgtcttcg cgcacgtctt cgacgcgctg aagcgggccg tgccggtccg cgcgctcgcg
      541 gccgtgtcgt tcgaggagga tccggacggg caggcggtcg tcgcgttcgg aggcgaggtg
      601 acgatcgagc gccgcgcgtt cctcgactgg ctctcgctcc agcgcgagct cgagtccccg
      661 ttcctcccct cgacaccccc gccgcgcatc gcccaggggc cgggccgcga gctcgtcctg
      721 aacccgcgcc tcggctgcca ggtgttcctg cctctccaga cgccggccct catcgcaggc
      781 gtcctcgtcg ccgagtcgga cgacaccgct ctcgtgaccc cgcaggtgct tcaggaactg
      841 tgcgtgttcg cggaccacct cgcgctctcg ctccaggacc ggaacctcag gcgccagatg
      901 caggccgtga acgagaggct ccaggggcgc gcggagatgc tccagcggat tctcgagctt
      961 tcgaacgagc tcaagacgca cctcacgctc gagcacctcg tccagagcat cgtccaggcc
     1021 gtccaccaca gcctcgggta tcgcaccgtc gcgttgtccc tctacgaggc gtccgagaac
     1081 gtgttcgagc gccgcgcgca gatgggcttc gacgacgctc tgccgggagc gccgccgcgc
     1141 cgcgtgccgc gcgacgaggt ggcccgctgg ttcgccgagc gctaccggat ctcgaagtcg
     1201 tacttcgtga gccacctcga ccggcaggag tcgatggacg actccggccg gatcgaccgg
     1261 cggcgccgcg gagccgcgcc cggcgcgtgg catccgaacg accttctctt cgtgcctctc
     1321 acggccggcg accagctcgt cggcgtcctg caggtcgacc agccgcgcag cggcctcgtg
     1381 ccgcgcctcg aggacgtcca ggcgctcgag atcttcgcga accaggccgt cacggcgctc
     1441 cagtccgcgc gcgcctacga gacgacgcgc cagatgtccg tgcgcgattc gctgacggag
     1501 gccttcaacc acaggtattt ccaggagacg ctctcccgcg agctcgcgcg ccacgagcgc
     1561 agcggccagc cgctcgcgct cgtcatgctc gacatcgacg acttcaagaa gatcaacgac
     1621 cggtggggcc atcccgtcgg ggacgtcgtc ctgcgcggac tcgtggagga gatgacgaaa
     1681 ggcgtccgcg agatggacac ggtggcgcgc tacggcggcg aggagttcgc gctcatcttt
     1741 cccgagacga cgcccgccaa ggcctgggtc gtcgcggacc gcctccgcaa gcgcgtcgcg
     1801 tcccggctgt tcgcggcgcc ccacgtcgag caggccctcg ccgtcacgat ctccctcggc
     1861 ctctcgacgt accccgagga cggctccacg aaacgcctcc tgatcgagcg tgcggaccag
     1921 gccctctacc aggccaagag gagcggcaag aactgcctcg tcacggccgc ctctctcgcc
     1981 gatctcaagg cgctgtgatc gaagacgagt accgcggccc ccggtggtgg cctctgtccg
     2041 cgctgcaggg caaggccgtc gcgggcatcg ggcccgcctg ggccggcctg acggcgctct
     2101 gcatcgggct cgcgatcctc gaggcccggc tcgactggag cggaatgccc gtgaggatcg
     2161 gccctgtgac ggtcggcgtc tcggtctatc cccccctgtc gatctccctc gtgctcgcgg
     2221 tgtggatcgg ccccctctgg ggactcgtcc cggcctggct cgccacgttc tcgagcgccc
     2281 tcacggcggg agttccgctg ccgtcggccg ccgtcttcgc gctcgcgacg cctgcggagg
     2341 tcttcctcct ctggggttcc atggtggcgt tgaacatcga cccggcgctc ccccgcttct
     2401 cggacctccg ccggttcctc gccgcagggc tcgtcgcggc gagcggctcg tcgcccgcga
     2461 tcctgatctg gaacgagtcg cgccatctcg atctcgtcgc gggccagagg ctctggcagg
     2521 gctggctcct cggagacctt ctccagatcg ccctcgtcgc cgcgccgttg ctccactttc
     2581 tcggcccccg cgcgaacgcg tttctcgaga agcggttcga cgcgcgcccg aaggtcgagt
     2641 tccgttaccg cgacggcgtt ctctggaccg ggctcgtctc cgtgacgctc gcggcggtgg
     2701 tcgccctcgg cctgaggatg gcgctcgtct cgctccggat cccggagacc gcggtgacgg
     2761 cgaggggcga gcccctcctg ccgcgcctgc gcgagatgac gctcttcgcc gggctcctgc
     2821 tcggcgtggt gctcgtcacg agcagcacgt tcggcgcctc cctcgcacgc ctcggggacc
     2881 gggaaaagtc gagcgcgcgc cgcgacccct tgacgggctg cctgaaccgc cgcgccttct
     2941 acgaagcgtt cgcgctcgag gcggaccgga gcgcgcgcct cgcggcggcg ctctcggtcc
     3001 tgacgatcga cgtcgaccgc ttcaaggtcc tgaacgacgc gcacggccac gcgttcggag
     3061 acgagatcct ccgccagctg gcccggcggc tcaccgcgct cgtgaggtcc cacgacctcg
     3121 tcgtccgctg gggcggcgag gagttcgtcg tcctcctccc ccatacgagc cccggggacg
     3181 cggccgccct cgccgagcgg atccgtgcgg gcgtcgaatc cccggagttc cgcgtggacg
     3241 ggcgggccgt gtccctgacg tgcagcgtcg gcgtcgccgg cacgcaggta ttcccggcga
     3301 gcgcggacga tctcgtcgca cagtccgacg ccgcgtgcta cgaagccaag cgcgcgggac
     3361 ggaaccgaac ggtcagcgtc gagatcgcgg cgctgaaagc gccttcggct tcgtgaccgc
     3421 cgggcagccc gcgaccgcga agcgcagcgg ccacgccgcg gcagggcccg cgtagtcgac
     3481 tccgacgcgg gggccggaga cgacgtcgaa gcgccaccgc gggccgcggg agaggacgat
     3541 ctcaggacct gcgagagacg ccccggacag cgccgtcgtc acgccgaggt aggcgcagag
     3601 cttcgcgggc ccggaggcct cccctcccct cttcaccttc aaagaaatct tctctttctc
     3661 ttcctccgga atccccgctc tcagcagaac tgcctcgggc accccttgtt cctgcgtcac
     3721 gacgttcagg cagtagtgca tgccgtagac gaagtagacg taggcgtggc cgccctcgcg
     3781 ccacatcggc gcgacgcgcg gcgtgcggcg cccgccccac gtgtgggcgg cgcggtcctt
     3841 cacgccgagg tacgcctccg tctcgacgag gatcaccgag cgcgtcgtcc cgtccggcag
     3901 ccgccgcgtg agacggcagc cgaggagggc gcgcgcgacg tcggtggtgt ccttccggta
     3961 gaaactcttc gggagcggct tctgcggggc cattgctatg ctcggcgcca tgatgcccga
     4021 gacgcgctcc gtcctcgtca ccggcggcgc cggattcatc ggctcgcacc tggccgacgc
     4081 cttcctcgag gccggatccc gcgtcacgat cctcgacgac ctctcgaccg gcgtcctcga
     4141 gcgcgtcccc gcgagggcgc ggttcgtgga gggcgacctg cgcacgttcg acttcgaggc
     4201 cctgctgcgc gccgagaagg tcgacacgat cctccaccat gccgcgcaga tcgacgtccg
     4261 caagtccgtc gaggatcccg tcttcgacgc ggaggtgaac gtcctcggga cgatccgcct
     4321 cgcccgggcc gcgctcgcct gcggcgtcac ccaggtcgtc ttcgcctcga gcggcggcgc
     4381 gatctacggc gatcccgagg gcttcgcggc agacgagaga caccccacga accccgtctc
     4441 cccgtacggc gtcgcgaagc tcgcgggcga gaagtacctc ttcggcctcg cccacggctc
     4501 ccccctcgtc gtcacggcgc tgcgatacgc gaacgtgtac ggcccgcgcc aggacgggcg
     4561 gggcgaggcg ggcgtcgtcg ggatctggat ggcgcgcctc ctcgagaacc agccgtgcgt
     4621 catctacggc gacgggctga actcgcggga cttcgtctac gtcggcgacg tcgtcgccgc
     4681 gaaccgcgcg gcctcggagc gccggctggg cggcgtcttc aacgtcggga ccggcgtcga
     4741 gacgaccgtg aaccggctct gggacgaggt cgcgcgcgcc tgcggctcgt ccgcgaaggc
     4801 cgcgcacgag gccgcaaagc cgggcgagca gcggcgcagc gtcctcgacg tcgcgaaggg
     4861 ccgcgccgcg ctcggcctcg gcgcgcccgt cagcctttcg gaggggctcc gccggacggc
     4921 ggcgtggttc cgggagcgcc cgaagccctg acccggttcg cgcccgggcc cttcgggtcg
     4981 cgcttgatca cgacaacggt cacgtcgtcc tccggggcgg ctccgccgcg ccacgacttc
     5041 accgcgttcg tgacgcggtg ggcgatttcc cgcgcgctct cgcgccggga gcccgcgacg
     5101 agcttccgga gccgcgccac cccgaactcg cggcccttcg cgtcgtgggc ctcgacgacg
     5161 ccgtccgtgt acagcagcac gatgtcgcac ggttccaggt gcgccgcacg gcggcggtag
     5221 acggcgtccg gcatcggccc caggaccatc cccgtcgacg tgaactccga gaaagtgccc
     5281 gtcgccgcat ggaagtggag cggtcccggg tgtcccgcgt tcacgtagac gacgtcgccg
     5341 tccgtgtcga actcaccgta gaagagcgac acgaacttcg tcgtgagacg gctcttggag
     5401 aagatccggt tcaaccgctc gatcgtccgc gcgatcttga tgtcgttgct gactcccatg
     5461 cgcagtccga cgtagatgtc gcgcacgagg agcgccgcga ggaggccgtg cccggacgcg
     5521 tcggcaatcg cgacaccgag gctctcgctg agcgggatga ggtcgtagaa atcgccgccc
     5581 acgagctcgg ccgggttcga gatgccgaag atgtcgaagt ccccgacctg cgggacgtga
     5641 cgcggcagga tcgacatctg gatgcggcgc gcgtcctgca ggaggccctc gaggtggccc
     5701 gtccggacct tcgagtcgag gaccgtgcgg atgatgccga gggcgagaag gagatcctct
     5761 tccctcacgc gcgggtgcac gtcgaaggag agaaggtact tttcctcgcc gaacgagacg
     5821 gccgcgaagc gcgtgacgcc gagcctctgc tcgaactcgg cgtcgaccga cgggtcgctg
     5881 cggtccatca ggacgatccc gtcctccacg gcgcgcacga tcgggccgta gctcgcgggc
     5941 acggcgaggc cggccggcgt ccgcttcgag cggccgaagc cgcgctcgag aatgtagacg
     6001 ttgtcctcgc ggagatagag acggccgccc aggatgccga gctcgttgcg gaaattgtgg
     6061 acgatgcctt cggcgacgcg gaggatcgtg agcgagacgt tgcccgtgcc ttcgatggcc
     6121 tcgagagttt tttcgacgtt tcggaggagc gcgcggtaat ccatgttcgg gtccgagtgt
     6181 acggcctcgg cgcgatatcg tccgcgcatg gacactctca cccgccgcca gctcgtcgcc
     6241 gcgtccgtcg cctccggaat cgccgccgcc gcgcgcccga tcgccgccgc gggaggcgcc
     6301 acctccgcgc ctgccgggga acgcccgttc gcgctcgccg aggcgacgat cgcggacctc
     6361 caggccggca tggcgtccgg caagtggacg gcccgctcga tcacccaggc atacctcgcc
     6421 cggatcgacg ccgtcgacgc gggcggcccg gcgctccgga gcgtcctcga gaagaacccc
     6481 gacgccctcg cgatcgccga cgccctcgac cgcgagcgga aggagaaggg cccgcgcggg
     6541 ccgctccacg ggatcccggt cctcgtcaag gacaacatcg acaccgccga ccgcatggcc
     6601 acgacggccg ggtcgctcgc gctcgtcggc gcgaagcctc cggccgacgc gttcgtcgtg
     6661 cggcggctgc gcgaggccgg cgccgtcctc ctcggcaaga cgaacctctc ggagtgggcc
     6721 aacatccggt cgtcgacgtc cacgagcggg tggtccggcc gcggggggct cacgcggaac
     6781 ccctacgcgc tcgaccggaa cccgtgcggc tcgagctccg gctcgggcgt cgcggccgcc
     6841 gcgaacctct gcgccgccgc cgtcgggacg gagacggacg gctcgatcgt gagcccgtcc
     6901 tcggcgaacg gcatcgtcgg ggtcaagccc acggtgggcc tcgtcagccg gaccggcatc
     6961 atcccgatct cgcactcgca ggacacggcc gggccgatgg cgcgcaccgt tcgcgacgcc
     7021 gcgattctcc tcggcgcgct cgcgggcgcc gacggggagg acgcggcaac cggcgcttcc
     7081 gggcaccgcg ccgagcccga ctacacggcg ttcctcgacc cggcaggcct ccggggcgcg
     7141 cgcatcggcg tcgtccgcgg cctcttcggg ttcctcgacc gcgtggacgc gctcatggcg
     7201 gaagcgctcg cggcgctcaa gcgcggcggc gccgagctcg tcgatcccgt cgagctcacc
     7261 tccgccgcga agatcggcga gccggagggc tccgtcctgt actacgaatt caaggccggc
     7321 ctgaacgcgt atctcgcgcg tctcgggccg ggcgcgccgg tcaagtcgct cgcggacgtc
     7381 atcgcgttca acgaaaaaca ctcgaaggag gagatgccgt acttcgcgca ggagacgttc
     7441 ctcaaggccg aggcgaaggg gccgctcacc tcgttcgagt acctcgaggc gctcgcgaag
     7501 tcccgccggc tcgcgcgcac ggaggggatc gacgcgacga tggaccggca caggctcgac
     7561 gcgctcgtcg cgccgacgct cggccccgcg tgcgtgacgg acctcgtcaa cggggaccgc
     7621 tggctgggcg acgcctcgac ggccccggcc gtcgccggct atccgcacgt cacggtgccg
     7681 gccggcttcg tcttcggcct gcccgtcggc ctctcgttct tcgg
//
LOCUS       DAOMFY010000184         7699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_2988_length_7699_cov_6.303637, whole genome shotgun sequence.
ACCESSION   DAOMFY010000184 DAOMFY010000000
VERSION     DAOMFY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_7699_cov_6.303637"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLB01_13600"
     CDS             complement(<1..1101)
                     /locus_tag="PLB01_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQQ78384.1"
                     /translation="MRPALRLACSAALAAALAGCSVRARVQSAAEAAGTKDRASVCVL
                     FVDGLSKEAFDHLLAEGALPNIRREIVDRALVIDNAVASVPSETYPNLGAMLTGLLPG
                     HHGIPANVWLDRRLRVREAHTNIFRTYAAGDFLTPSARTLFERLPADTVAVTTPIARG
                     VTVHVKNTVSVMASYARYDWPFLDRKTLDDMGDAYAGAAASGRLPSLVWGHLLGPDEV
                     AHAEGPGSEDFHATLASIDKAFGRLVRRLKKMKAADRVLFVLVGDHGNRSYHTFVDAN
                     ELVNRVLRANPTEADCTNGGCVLVPVPRKDRTYDVGDAMIAVGAYRGAMIWLPAERPP
                     EEVPEAFRTRKQKRPKGPAAPRLRMPPVSAFAA"
     gene            1154..2026
                     /locus_tag="PLB01_13605"
     CDS             1154..2026
                     /locus_tag="PLB01_13605"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQQ78385.1"
                     /translation="MPPFRGADLHCHSTFSDGVDAPARLVGHAVAAGLSVLALSDHDA
                     VHGLPEFEAAAKGTGLVTVAASELSTRCNGDDVHVLGLFLDPEEPGMKAQLERFRVDR
                     DTRGEAMVEKLAALGMPLDLAAIREVVGGGSFGRPHIARAMLAKGYVRTFDEAFDTWL
                     AHGKPAYVPKPKWTLEEAIAAIRTAGGLSVIAHPVWYKAPEQVVAIGVAAGLDGLEVY
                     HVDQAGKENEFGKLAERHGLLKSAGSDYHGPAEGKRRVGTCRLDAAGWERMVAAAEAR
                     RAESGRPPLDLSPR"
     gene            2075..3430
                     /gene="dnaB"
                     /locus_tag="PLB01_13610"
     CDS             2075..3430
                     /gene="dnaB"
                     /locus_tag="PLB01_13610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQQ78386.1"
                     /translation="MSALAPAAAETMPSRPDAERSLLGAVLIDGALLSRVMEFLAPDD
                     FAAEPHRVIYEAALALADRREGIDLVMIQSELEKTGRLERAGGPAYLSSLVDVVPDVE
                     NVEGYARLVREASLRRLVILQSRRAIREAVSAPDAETVLENAQRELVEIAKGSIKGGF
                     VKLSRIAEKNEEEIQNLHGRGGMLTGLPSGFTRLNALTQGLQKTDLIVVAARPGMGKT
                     AFALNIAATLTIREKYVVGFFSLEMSAQQLGFRIQCSEARVNLQRLREGRLSKEEKKA
                     LFFSTQQMREANLFVDDTPAITLLEMRARALQLKQRHGLHALFVDYLQIMGTRGKVEN
                     RTQEVSAFSRGLKALAKELDVPVIALSQLSRRTEQRGAEKEPVLSDLRESGAIEQDAD
                     VVLFLSRPEYYDKDTEQKNVCDVIIAKQRNGPTDRFTLGWSGEYTRFSDLAMMSEGPG
                     L"
     gene            3427..4629
                     /gene="alr"
                     /locus_tag="PLB01_13615"
     CDS             3427..4629
                     /gene="alr"
                     /locus_tag="PLB01_13615"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQQ78387.1"
                     /translation="MTPALRPTRAVVDLDAVASNFHTLGAGLPRGCGVLPVVKADAYG
                     HGAVHVARRLAKEGAACFAVAVVEEGLELRRAGITQPILVMGWIGKDQIGSLVEGGLT
                     PNIHSVEQAEEMKIFLEGEEGRGKTLPVHLKLDTGMTRLGLRPEDLPRALEILEGLKS
                     KIFLEGVFQNFASAYDVTSEQNADQTRIFLEGLERVRAAGHRPSMIHMDNSGGTLARL
                     ASSPSKKSSSSVVTHVRPGLALYARIPEVPSAAGETLQDVMSFVSVVDQVKTVPAGTK
                     VGYAGTFVCSRTTTLAVVAAGYADGVRRSLSNKGQVLVGGTRCPIAGRISMDLTEVDV
                     TDLPRPPGRGDEAVFFGTQSGARLGVEEVAATADTVAWELLCGVGPRVPRIVVESGRE
                     VARVSRFL"
     gene            4626..5396
                     /locus_tag="PLB01_13620"
     CDS             4626..5396
                     /locus_tag="PLB01_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78388.1"
                     /translation="MKRVLEGIGRPVLRLLEGVGRFFELLGQTIRGFFLPPYQISMTA
                     KQVVRVGVDSVPVVFLTALFTGGVLALQTFKGFQRFHAEGYIGSVVALSMLRELGPVL
                     TGLMVTGRAGSAMAAELGSMRVTEQIDALVSLATDPVQYLFVPRVLAAIIVSPMLVVL
                     ADATGILGGYVVAVQYLGANPVVYLNNTFQYLDMDDLTSGLIKSAVFGLLFSMIGCQM
                     GYDTKGGAEGVGTSTTAAVVLGSLAILIADFFLTKVLF"
     gene            5396..6220
                     /locus_tag="PLB01_13625"
     CDS             5396..6220
                     /locus_tag="PLB01_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78389.1"
                     /translation="MAAPAPSGVPSIRLRGVTKAFGKKVVLDHLDLAVEKGESLVLLG
                     QSGIGKSVLIKHVVGLLKPDAGSVEIDGEDLWAVSSRERDVLRRKFGMSFQEGALFDS
                     MSVGENIAFPLVRHTKKTKKEIADRVAECLELVRLPGIEDKRPSELSGGMRRRVGFAR
                     AIALQPQILLFDEPTTGLDPITTDVLVRVIEEMRKRLNPTAITITHDLKVAFGIADRV
                     GLLAHGKIAIDLPPKEFEASSDPRVAAFVKGDSELSPPELEPESRPAAAPPAAAPL"
     gene            6231..7670
                     /locus_tag="PLB01_13630"
     CDS             6231..7670
                     /locus_tag="PLB01_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQQ78390.1"
                     /translation="MTTTAKIGAFFVAVLVALAILILKIEDIHFGKKARTNAVDAHFK
                     DVAGLDDKSAVRIAGVRIGKVDGISLRPDGTAVVHLALDPGVELREGASAMVRSLGLL
                     GDKYVELQPGTPDAPRLAQGALLEGGSTTGFDDLTKMASDIGKDLKELSSALAGSMGG
                     KQGEEKINRIVDNIGALAEALKDLVEHNRNNIDVTLANLKEFSGQMRETLARMDRILD
                     ENRATFKSTVSNADQISEKLKTTADNLNSITGKIDTGQGTIGKLLNDDETHKNLNDAL
                     SSVRTGVDSLNQTLSRMNRIQLDLGFRAEYVSRGGDGKYWFTLDAIPRDNKFYRLEIG
                     ATPKGMRQNIDETTTVTFPNGSEQTIHTTGERYVDQLLLSLQLGYRLKDTILRAGLIE
                     SRGGAAIEQTFKADTFRLAGEVWDFSRDNYRGHVKLYGRWNASPNLYVTGGADDVLNP
                     GLRSAFIGGGIRWKDEDLKGLIGSIPVPK"
BASE COUNT         1203 a         2682 c         2678 g         1136 t
ORIGIN      
        1 ggcggcgaac gcggagacgg gcggcatcct caggcgcggc gcggcggggc ccttcggccg
       61 cttctgcttg cgcgtccgga acgcttccgg cacttcctcc ggcgggcgct cggcggggag
      121 ccagatcatc gcgccccggt acgcgccgac ggcgatcatc gcgtcgccga cgtcgtacgt
      181 cctgtccttg cggggcacgg gcacgaggac gcagcccccg ttcgtgcagt cggcttcggt
      241 cggattcgcg cggaggacgc ggttcacgag ctcgttcgcg tccacgaacg tgtggtacga
      301 gcggttgccg tggtcgccga cgaggacgaa gagcacgcgg tccgcggcct tcatcttctt
      361 gagccgcctc acgagccggc cgaacgcctt gtcgatcgac gcgagcgtcg catggaagtc
      421 ctcgctgccg gggccctcgg cgtgcgcgac ttcgtccggc ccgagcaggt ggccccagac
      481 gaggctgggc aggcggccgg aggcggccgc gcccgcgtac gcgtcgccca tgtcgtcgag
      541 ggtcttccgg tccaggaacg gccagtcgta ccgggcgtac gacgccatca ccgagaccgt
      601 gttcttcacg tgcacggtga cgccccgcgc gatgggcgtc gtgacggcga ccgtgtcggc
      661 gggcagccgc tcgaacagcg tgcgcgccga cggggtcagg aagtcgcccg ccgcgtacgt
      721 gcggaagatg ttcgtgtggg cttcccggac gcgcaggcgg cggtcgagcc agacgttcgc
      781 ggggatcccg tggtgcccgg gcagaaggcc cgtcagcatc gcgccgagat tcgggtacgt
      841 ctcgctcggg accgaggcga ccgcgttgtc gatgacgagc gcccggtcga cgatctcgcg
      901 gcggatgttc gggagcgccc cttcggcgag gaggtggtcg aaggcctcct tcgagagccc
      961 gtcgacgaag aggacgcaga ccgaggcgcg gtccttcgtg ccggcggcct ccgcggcgct
     1021 ctggacgcgc gcgcgcacgg agcacccggc gagggccgcc gcgagggcgg cggagcaggc
     1081 gagacggagg gccgggcgca cgggcgaagg gtacaggagc ggaccgggcc ggctcccgcg
     1141 ctaccatgcc cggatgcccc cgttccgcgg cgccgacctt cactgccatt cgacgttctc
     1201 cgacggagtc gatgcacccg cgcgcctcgt cggccacgca gtcgcggcgg gcctgtccgt
     1261 cctcgccctc tccgatcacg acgcggtgca cggcctgccc gagttcgagg ccgcggcgaa
     1321 ggggacgggc ctcgtgaccg tcgcggcgag cgagctctcg acgcgctgca acggcgacga
     1381 cgtgcacgtc ctcggcctct ttctcgaccc ggaggagccc gggatgaagg cccagctcga
     1441 gcgattccgc gtggaccgcg acacgcgcgg cgaggcgatg gtggagaagc tcgccgccct
     1501 cgggatgccg ctcgacctcg ccgcgatccg cgaggtggtc ggcgggggct ccttcgggcg
     1561 cccccacatc gcgcgcgcga tgctcgcgaa gggctacgtg aggacgttcg acgaggcctt
     1621 cgacacgtgg ctcgcgcacg gaaagcccgc ctacgtcccg aagccgaagt ggacgctcga
     1681 ggaggcgatc gccgcgatcc ggacggcagg ggggctctcc gtcatcgcgc accccgtctg
     1741 gtacaaggcg cccgagcagg tcgtcgcgat cggcgtcgcc gccggcctgg acggcctcga
     1801 ggtctaccac gtcgaccagg caggcaagga gaacgagttc ggcaagctcg ccgagcgcca
     1861 cggcctcctg aagtccgcgg gctccgacta tcacggcccc gccgagggga agaggcgcgt
     1921 cggaacgtgc cgcctggacg ccgccggatg ggagcgcatg gtcgcggcgg ccgaggcgcg
     1981 ccgcgccgag tccggccggc cgcccctcga cctttcgccg cgctgacgcc ccttttccgg
     2041 gcgaggcccg cgcgcgatat tctccccggt cccgatgtcc gccctcgccc ccgccgccgc
     2101 ggaaaccatg ccgtcgaggc ccgacgccga gcggagcctc ctgggcgccg ttctgatcga
     2161 cggggccctc ctctcgaggg tcatggagtt cctcgccccg gacgacttcg ccgccgagcc
     2221 gcaccgcgtg atctacgagg ccgcgctcgc cctcgcggac cggcgcgagg gcatcgacct
     2281 cgtcatgatc cagagcgagc tcgagaagac gggccggctg gagcgggcgg gcggcccggc
     2341 ctatctctcg tccctcgtgg acgtcgtccc ggacgtcgag aacgtcgagg gctacgcccg
     2401 gctcgtccgc gaggcctcgc tccgccgcct cgtgatcctc cagtcgcgcc gcgccatccg
     2461 cgaggcggtc tccgccccgg acgccgagac cgttctcgag aacgcccagc gcgagctcgt
     2521 cgagatcgcg aaggggtcca tcaagggcgg gttcgtgaag ctctcacgga tcgcggagaa
     2581 gaacgaggag gagatccaga acctccacgg ccgcggcgga atgctcacgg ggctgccgtc
     2641 cgggttcacg cgcctgaacg cgctcacgca gggcctccag aagaccgacc tgatcgtcgt
     2701 cgcggcccgc cccggaatgg gcaagaccgc gttcgcgctg aacatcgcgg cgacgctcac
     2761 gatccgcgag aagtacgtcg tcggcttctt ctcgctcgag atgtcggccc agcagctcgg
     2821 cttccgcatc cagtgctccg aggcgcgcgt gaacctccag cggctgcgcg agggacggct
     2881 cagcaaggaa gagaagaagg cgctcttctt ctcgacgcag cagatgcgcg aggcgaatct
     2941 cttcgtggac gacacgcccg cgatcacgct cctcgagatg cgcgcgcggg cgctgcagct
     3001 caagcagcgg cacggcctgc acgccctgtt cgtggactac ctccagatca tggggacgcg
     3061 cggcaaggtg gagaaccgca cgcaggaggt cagcgcgttc tcgcggggcc tcaaggcgct
     3121 cgccaaggag ctcgacgtcc cggtcatcgc cctctcgcag ctctcgcgcc gcacggagca
     3181 gcgcggagcc gagaaggagc ccgtgctctc cgacctgcgc gagtcgggcg ccatcgagca
     3241 ggacgccgac gtcgtcctgt tcctctcgcg ccccgagtac tacgacaagg acacggagca
     3301 gaagaacgtc tgcgacgtca tcatcgcgaa gcagcgaaac ggcccgacgg accgcttcac
     3361 gctcgggtgg tcgggcgagt acacgcgctt ctcggacctc gcgatgatga gcgaaggccc
     3421 gggcctgtga cgcccgcgct ccggccgacg cgggccgtcg tcgacctcga cgcggtggcg
     3481 tcgaacttcc acacactcgg ggccggcctg cctcgaggat gcggcgtcct cccggtcgtc
     3541 aaggccgacg cgtacgggca cggcgcggtg cacgtcgccc ggcgcctcgc gaaggagggc
     3601 gcggcctgct tcgccgtcgc cgtcgtcgag gaaggcctgg agctgaggcg cgccgggatc
     3661 acgcagccga tcctcgtcat ggggtggatc gggaaggatc agattggctc cctcgtagag
     3721 ggtgggctga cccccaacat ccattcggtc gaacaggccg aggagatgaa gattttctta
     3781 gaaggtgaag aggggagggg gaagaccctt cccgtccacc tcaagctgga caccggcatg
     3841 acgcgcctcg gcctgcgccc cgaggacctg ccgcgggctc tcgaaatcct cgaggggttg
     3901 aagtcgaaga ttttcctcga aggtgtattc cagaacttcg cctcggcgta cgacgtcaca
     3961 tcggagcaga acgcggacca gacgaggatt ttcttagaag gtttggagag ggtccgcgcg
     4021 gcgggccatc ggccgtcgat gatccacatg gacaactcgg gtggaacgct ggcccgtctg
     4081 gcctcttcac cttcaaagaa atcttcctct tcggtcgtga cccacgtgcg gccgggtctc
     4141 gcgctgtacg cacgaattcc cgaggtgccg tctgcggcag gggagacact gcaggacgtc
     4201 atgtcgttcg tttccgtcgt cgaccaggtc aagaccgtcc ccgccggaac gaaggtcggc
     4261 tacgccggga cgttcgtctg ttcgcgcacg acgacgctcg cggtcgtcgc cgcgggctac
     4321 gcggacgggg tgcggcggtc gctctcgaac aagggacagg tcctcgtcgg cgggacgcgc
     4381 tgcccgatcg cgggccgcat ctccatggac ctcacggagg tcgacgtgac cgacctgccg
     4441 cgtcctcccg ggcgcggcga cgaggccgtg ttcttcggga cgcagtccgg cgcgcgactg
     4501 ggcgtggaag aggtggcggc gaccgccgac accgtggcgt gggaactcct gtgcggcgtc
     4561 ggcccgcgcg tgccgcgcat cgtcgtcgag agcggccgcg aggtggcgcg cgtgtcgagg
     4621 ttcctgtgaa gcgcgtcctc gaaggcatcg ggcgccccgt tctccgcctc ctcgaaggcg
     4681 tgggccgctt cttcgagctg ctcggccaga cgatccgcgg cttcttcctg cccccgtacc
     4741 agatctcgat gacggccaag caggtcgtgc gcgtcggcgt ggattccgtc cccgtcgtct
     4801 tcctgacggc gctcttcacc ggcggcgtcc tcgcgctcca gacgttcaag ggcttccagc
     4861 gcttccacgc cgagggctac atcgggtcgg tcgtcgcgct ctcgatgctg cgcgagctcg
     4921 gacccgtcct cacggggctc atggtgaccg gccgcgcggg cagcgccatg gcggccgaac
     4981 tcggctcgat gcgcgtcacc gagcagatcg acgcgctcgt ctccctcgcg acggacccgg
     5041 tccagtacct cttcgttccg cgcgttctcg cggcgatcat cgtctccccg atgctcgtcg
     5101 tcctcgcgga cgcgacgggc atcctcggcg gctacgtcgt cgcggtccag tacctcggcg
     5161 cgaacccggt cgtgtacctg aacaacacgt tccagtacct ggacatggac gacctgacgt
     5221 cgggactcat caagtccgcc gtcttcgggc tcctgttctc gatgatcggg tgccagatgg
     5281 gctacgacac gaagggcggg gcggaaggag tcggcacctc gacgacggcc gccgtcgtcc
     5341 tcggctcgct cgcgatcctc atcgcggact tcttcctcac gaaggtgctc ttctgatggc
     5401 cgcccccgcg ccctccggcg tcccgtcgat ccgcctgcgc ggcgtcacga aggcgttcgg
     5461 gaagaaggtc gtgctcgacc acctcgacct cgccgtcgag aagggggagt cgctcgtcct
     5521 cctcgggcag tccggcatcg gcaagagcgt cctcatcaag cacgtcgtgg ggctcctgaa
     5581 gcccgacgcg ggctcggtcg agatcgacgg cgaggacctc tgggcggtct cgtcgcgcga
     5641 gcgcgacgtc ctccggcgga agttcgggat gtcgttccag gagggcgcgc tcttcgactc
     5701 catgagcgtg ggggagaaca tcgcgttccc tctcgtgcgg cacacgaaga agacgaagaa
     5761 ggagatcgcg gaccgcgtcg ccgagtgtct cgagctcgtc cgccttcccg ggatcgagga
     5821 caagcgcccg tccgagctgt ccggcggcat gcggcggcgc gtcgggttcg cccgggccat
     5881 cgccctccag ccgcagatcc tcctcttcga cgagccgacg accgggctcg acccgatcac
     5941 gacggacgtc ctcgtccgcg tcatcgagga gatgcgcaag cgcctgaacc cgacggcgat
     6001 cacgatcacc cacgacctca aggtcgcctt cgggatcgcg gaccgcgtcg gcctcctcgc
     6061 gcacggcaag attgcgatcg acctgccccc gaaggagttc gaggcctcgt cggacccgcg
     6121 cgtcgcggcg ttcgtcaagg gcgacagcga gctgtccccg cccgagctcg agccggagag
     6181 ccgcccggcg gccgcgccgc cggcggcggc acccttatga tggaggccgc atgacgacca
     6241 ccgcgaagat cggcgccttc ttcgtcgccg tgctcgtcgc gctcgccatc ctgatcctca
     6301 agatcgagga cattcatttc ggcaagaagg cgcgaacgaa cgcagtcgac gcgcacttca
     6361 aggacgtcgc gggcctcgac gacaagtccg cggttcggat cgccggggtg cgcatcggga
     6421 aggtggacgg catctcgctg cgcccggacg ggacggcggt cgtgcacctc gcgctggacc
     6481 ccggcgtcga actccgcgag ggggcgtccg cgatggtgcg gagcctcggc ctcctgggcg
     6541 acaagtacgt ggagctgcag ccgggaacgc cggacgcgcc gcggctcgcc cagggcgcgc
     6601 tcctcgaggg cggctccacg accgggttcg acgacctcac gaagatggcg tcggacatcg
     6661 ggaaggacct caaggagctg tcttcggcgc tcgccgggtc gatggggggc aagcagggcg
     6721 aggagaagat caaccggatc gtcgacaaca tcggcgcgct cgccgaggcg ctcaaggacc
     6781 tcgtcgagca caaccggaac aacatcgacg tgacgctcgc gaacctcaag gagttctcgg
     6841 gccagatgcg agagacgctg gcgcgtatgg accggatcct cgacgagaac cgcgccacct
     6901 tcaagtcgac ggtctcgaac gccgaccaga tctccgagaa gctgaagacg acggccgaca
     6961 acctgaactc gatcaccggg aagatcgaca cgggtcaggg cacgatcggc aagctcctga
     7021 acgacgacga aacgcacaag aacctcaacg atgcgctgtc ctcggtgaga accggcgtcg
     7081 attcgctcaa ccagacgctc tcgcggatga accggatcca gctcgacctc gggttccgcg
     7141 ccgagtacgt gagccgcggc ggcgacggga agtactggtt cacgctggac gcgatccccc
     7201 gcgacaacaa gttctaccgt ctcgagatcg gggcgacgcc gaagggcatg cggcagaaca
     7261 tcgacgagac gacgaccgtc acgttcccga acgggagcga gcagacgatc cacacgacgg
     7321 gcgagcgata cgtcgaccag ctcctgctct cgctccagct cggctaccgc ctgaaggaca
     7381 cgatcctccg tgcgggcctc atcgagtcgc gcggcggcgc cgcgatcgag cagacgttca
     7441 aggccgacac gttccggctc gccggcgagg tgtgggactt ctcgcgggac aactaccgcg
     7501 ggcacgtgaa gctctacggg cgctggaacg cgagcccgaa cctctacgtg acgggcggcg
     7561 ccgacgacgt cctgaacccc ggcctgcgct ccgcgttcat cggaggcggg atccgctgga
     7621 aggacgagga cctcaagggt ctcatcggct cgatcccggt tcccaagtaa gtggcgcgaa
     7681 agcccgcgac cgtctttgc
//
LOCUS       DAOMFY010000185         7660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3006_length_7660_cov_6.229323, whole genome shotgun sequence.
ACCESSION   DAOMFY010000185 DAOMFY010000000
VERSION     DAOMFY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3006_length_7660_cov_6.229323"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1576
                     /locus_tag="PLB01_13635"
     CDS             119..1576
                     /locus_tag="PLB01_13635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQQ78391.1"
                     /translation="MDLTELLKFVTKKEASDLHLKPGRPPLLRVNGRLVPLKTDPLQP
                     KEIEDMVLPILTPYQRDKLQQNYAVDIGYGITGVARFRGNIYLQRGTWTAVFRRIPFQ
                     IPQVAALNLPDVLETFADLPAGLVLVTGPTGSGKSTTLAALVKLITERHPVHVVTIED
                     PIEYLFQDNMASVSQREMGTDTISFQEALKNVMRQDPDVIMVGEMRDWETMSTAITAA
                     ETGHLVFSTLHTNSAAQTIDRIMDSCPPSQQNQVRRQLALVLRAIVSMQLIERVDGKG
                     LIPVVEVLVASPKIAKQIETGETKEILEEVESSVAYFKMQSMNQSLIALLVNGAIDVD
                     AAMKLTTDPEDLSLKLRKLFPKIEERGRGGIMASANDFSAITELMDIKRLYEEQEEKW
                     RQRVSEKDEVIAALEHDIEERERQVQAKAQMGAEAESEIQRLRADAERQRQEMAAKVA
                     QLNERIRDLNQRLLGTGGQSAVAATPGNPQGFFKR"
     gene            complement(1584..3401)
                     /locus_tag="PLB01_13640"
     CDS             complement(1584..3401)
                     /locus_tag="PLB01_13640"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQQ78392.1"
                     /translation="MRTTRFTVAMPRPETHLYEITMEIDTRGASTVDLVLPVWTPGSY
                     LVREFSRHVRDLRAREVSSNERGVGAGARGGGRPIAAEKISKNTWRFSLPSKKSSSSS
                     SVLVSYRVYAHELTVRTSHLDSTHGYGNGANLFFYVDGRKDEPQEVVFRLPRGWRTSM
                     ALPARGGVFRAASYDELVDSPFECGTHRTFDFRVKGVPHTLAIWGSGNEDAARLVKDL
                     KKLVTEAASLFGGLPYERYLFLAHLAPGARGGLEHRASQSVAVDPWAFQPAKAYRGTL
                     LLFSHELFHAWNVKRIRPEALGPFDYTKEVHTKDLWAMEGITSYYEVLLAVRAGLLTP
                     AQGFEEWTTSWSGHLETPGRDVQSAEMASFDTWIRFYRPDENSVNVAESYYRRGQLLG
                     LALDLTIRGATAGRRGLDDVMRLLWRRWGSKGKGYPEGAVEDAAASVLASRAKARRFF
                     DRYVRGTGTADLARLLPAAGLELRLVPEAEEGVTDESPVKTRGDFGWKTKTENGRLVV
                     AEVREGGAAMRAGMSAADEIVAIDGVRASEDFLRRKAIEAGPGARLRVTVFRRERLRE
                     LGLVLGARKAGVWKIVPAKGAAPAARRLARRWLHVPVSG"
     gene            complement(3409..4200)
                     /locus_tag="PLB01_13645"
     CDS             complement(3409..4200)
                     /locus_tag="PLB01_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ78393.1"
                     /translation="MTVACLDAGATRPGGAPPLVLLHGIGADHGEWALSLAMLARKRR
                     ILAFDLLGHGKSDKPSGSGVVYRVRLLAEAVRGGIEALPDAPERVDLLGHSLGGAVAL
                     DVVRRHARLVRRLVLVDALGLPVARSIDPLAASLSFVPGSFEDSRRLLATSVNNRFLA
                     HPLVALAAAVYKGRRKNRPQLLKLAASLAAGEDAFSPRDLARIPHETLVLWGDRDRIF
                     PLATGRAMARAIPNARLEVMPRCGHVPPTERPVAFARRVVAFLDS"
     gene            complement(4230..4838)
                     /gene="lexA"
                     /locus_tag="PLB01_13650"
     CDS             complement(4230..4838)
                     /gene="lexA"
                     /locus_tag="PLB01_13650"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQQ78394.1"
                     /translation="MALTLTPRQKEIADFIRRYRSKHGVSPTQREICEEFGYSSFGTL
                     QKHIRLLLEKGVLVRDWNKRRSLALAEEDRPVGAVEIPLAGRIAAGQPIEVEPQGESV
                     SVPEALTRKGQNYVLRVSGHSMIDDGIHDGDFVVVNRREKAANGEMVAALVNGEATLK
                     RFYNEGDGRVRLQPANDRMAPIYAPEGDVKVQGVVVGLMRKY"
     gene            5058..5561
                     /locus_tag="PLB01_13655"
     CDS             5058..5561
                     /locus_tag="PLB01_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQQ78395.1"
                     /translation="MPDSAKPTLPAPPARGSFMRKAGRWTIGVLAAAIVLSAAVLWLY
                     NRAFTSERPVVRASRLTSGNWVFPAQVAVFADRVARYKPRFLGHTEDSITLRQIASVK
                     VHAGPVFADVVIDTTGGSPPVLIHGLRKKDAEALSASIASAQSTLPMPVRPVAPEAPA
                     APPPATR"
     gene            5621..6196
                     /locus_tag="PLB01_13660"
     CDS             5621..6196
                     /locus_tag="PLB01_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78396.1"
                     /translation="MKNVLKMTALVLCCVALVACSQKGPAEAALTAATTAVDGVKAEA
                     AKYVPDQAKSLMASLTAAREAFDKGDYKMALEAATAIPAKAKEAAAAVAAKKDELTKS
                     FTALTASLPATVEQIKAKVAELAAMKKLPKDMDKAKVEAAKTGVEAAQKTWAEANEAF
                     KAGNLMDAVKKGNDVKGQADQLLAALNIAAK"
     gene            complement(6259..7443)
                     /gene="argJ"
                     /locus_tag="PLB01_13665"
     CDS             complement(6259..7443)
                     /gene="argJ"
                     /locus_tag="PLB01_13665"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HQQ78397.1"
                     /translation="MRLPKGFRASGVYAGVRKKPLPDVALLAAENGANAAALFTTNRF
                     QAAPVVLGRADLRASGGRVRAVVVNAGCANAVTGEAGMAAARRVRDAAAKLLGVAKRE
                     VLVSSTGVIGVVLPDEKVRAALPEAKAKLSVSGVDAASRAIMTTDAGPKVARATFTWK
                     GRRGSVVGIAKGAGMIHPNMATMLAFVTTDAPAAPACLKAVLTHAADRSFHAISVDGD
                     TSTNDTLLLMASGAMGGPEMASAADAAPLRAAVTAVCRKLAWLMVRDGEGATRVLDVS
                     ITGARTEADAKAAAHAVATSPLVKTALNGGDPNWGRILAAVGRSGARFSASKVSLRLG
                     RLVLVDRGLPVAYREKDAAKVFRQERVPVVVDLGAGKATAFKLASDLGHAYVSCNADY
                     RS"
     gene            complement(7440..>7660)
                     /locus_tag="PLB01_13670"
     CDS             complement(7440..>7660)
                     /locus_tag="PLB01_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQQ78398.1"
                     /translation="AHEIVGRRIAEAEALGVTLLALGPKDEITDKDLQALDVRRALAK
                     RDVTGGTAPRQVARAARAAVRRLSGGAS"
BASE COUNT         1227 a         2738 c         2596 g         1099 t
ORIGIN      
        1 cgccggggag atccgcggcg tccgcgccgc ccgcgcctgg ctcgccgcgc gtgcccgggt
       61 tggaaaacgc acgcgccggc tggtacccta gagagtcccc ttcgaggaca ttcgaacgat
      121 ggacctgacc gagcttctga agttcgtcac gaagaaagag gcgtcggacc ttcacctgaa
      181 gcccggccgt ccgcccctcc tgcgcgtgaa cgggcggctc gtcccgctca agacggaccc
      241 gctgcagccg aaggagatcg aggacatggt cctcccgatc ctgacgccgt accagcggga
      301 caagctgcag cagaactacg ccgtcgacat cggctacgga atcacgggcg tcgcgcgctt
      361 ccgcggcaac atctacctcc agcgcggcac gtggaccgcc gtcttccggc gcatcccgtt
      421 ccagatcccg caagtcgccg ccctcaacct tcccgacgtc ctcgagacgt tcgcggacct
      481 tcccgcaggg ctcgttctcg tgaccgggcc gacgggatcg ggcaagtcga cgacgctcgc
      541 ggccctcgtg aagctcatca cggaacgaca ccccgtccac gtcgtcacga tcgaggaccc
      601 gatcgagtac ctcttccagg acaacatggc ctcggtctcg cagcgcgaga tggggacgga
      661 cacgatctcc ttccaggaag cgctcaagaa cgtgatgcgg caggacccgg acgtgatcat
      721 ggtcggcgag atgcgcgact gggagacgat gtcgaccgcg atcacggcgg ccgagacggg
      781 gcacctcgtc ttctcgacgc tgcacacgaa cagcgccgct cagacgatcg accgcatcat
      841 ggactcctgc ccgccctcgc agcagaacca ggtgcggcga cagctcgccc tcgtcctgcg
      901 cgccatcgtt tcgatgcagc tcatcgagcg cgtggacgga aaggggctga tccccgtcgt
      961 cgaggtcctc gtggcctccc ccaagatcgc caagcagatc gagacgggtg agacgaagga
     1021 gatcctcgaa gaggtcgaat ccagcgtcgc ctatttcaag atgcagtcga tgaaccagtc
     1081 gctcatcgcg ctcctcgtga acggcgcgat cgacgtcgac gcggcgatga aactcacgac
     1141 cgacccggaa gacctgtccc tcaagctccg caagctcttc ccgaagatcg aggagcgcgg
     1201 ccgaggagga atcatggcgt ccgcaaacga cttctccgcg atcaccgagc tcatggacat
     1261 caagcgcctg tacgaagagc aggaagagaa gtggcggcag cgggtctcgg aaaaggacga
     1321 ggtcatcgcg gccctcgagc acgacatcga ggagcgcgag cggcaggtcc aggcgaaggc
     1381 ccagatgggt gcggaggccg aaagcgagat ccagaggctg cgcgccgacg ccgagcgcca
     1441 gcgtcaggag atggcggcga aggtcgccca gctcaacgag cgcattcgcg acctgaacca
     1501 gaggctcctc ggcaccggcg ggcagtccgc cgtggccgcg acgcccggga acccgcaggg
     1561 cttcttcaag cgctgacgga ggatcagccg ctcacgggaa cgtgcagcca gcgccgcgcg
     1621 agccgtctcg cggcaggcgc cgcgcccttc gccggcacga tcttccagac gcccgccttt
     1681 cgcgccccaa ggacgagccc gagctcccgg aggcgttcgc gccggaacac ggtgacccgg
     1741 agccgcgcac cgggtccggc ctcgatcgcc ttccttcgaa ggaaatcctc cgacgcccgc
     1801 accccgtcga tcgccacgat ctcgtcggcc gcgctcatgc cggcgcgcat ggcggcgccg
     1861 ccctcgcgaa cctcggccac gacgagcctg ccgttctccg ttttcgtctt ccagccgaag
     1921 tccccccggg tcttcacggg gctctcgtcg gtcacgcctt cttccgcctc gggaacgagc
     1981 ctcagctcga ggccggccgc cgggaggagc cgcgcgaggt cggccgtccc ggttccgcgc
     2041 acgtagcggt cgaagaaccg gcgcgccttc gcacgcgacg cgagcacgct agccgccgcg
     2101 tcctcgacgg ccccctccgg gtagcccttc cccttcgagc cccagcggcg ccagaggagc
     2161 ctcatgacgt cgtccagccc gcgccggccc gcggtcgcgc cccggatcgt gaggtcgagc
     2221 gcgagcccga ggagctggcc gcgacggtag tagctctcgg cgacgttcac ggagttctcg
     2281 tcgggccggt aaaagcggat ccaggtgtcg aaggaagcca tctcggcgct ctgcacgtcg
     2341 cggccgggcg tctcgaggtg acccgaccac gacgtcgtcc attcctcgaa accctgggcc
     2401 ggcgtcagga ggccggcgcg cacggcgaga aggacctcgt agtacgacgt gatcccctcc
     2461 atcgcccaga ggtccttcgt atggacctcc ttcgtgtagt cgaacggacc gagcgcctcg
     2521 ggtcggatcc gcttgacgtt ccaggcgtgg aagagctcgt gcgagaacag cagcagcgtg
     2581 ccgcggtacg ccttcgccgg ctggaacgcc cacggatcga ccgcgacgga ctgcgaggcg
     2641 cggtgctcga ggccgccgcg cgcgcccggc gcgaggtggg cgaggaacag ataccgctcg
     2701 tacggaaggc cgccgaagag cgacgcggcc tccgtgacga gcttcttcag gtccttcacg
     2761 aggcgcgccg cgtcctcgtt gccgctgccc cagatcgcga gcgtgtgcgg gacgcccttc
     2821 acgcggaagt cgaacgtgcg gtgcgtgccg cactcgaacg gagagtccac gagctcgtcg
     2881 tacgacgcgg cccggaacac gccgcctcgc gcgggaaggg ccatggaggt cctccagccg
     2941 cgcgggaggc ggaagaccac ttcctgcggc tcgtccttcc gcccgtcgac gtagaagaac
     3001 aggttcgcgc cgttgccgta gccgtgcgtg gagtcgaggt gcgacgtgcg cacggtcagc
     3061 tcgtgggcgt agacgcggta agagacgagg accgaagagg aagaggaaga tttcttagaa
     3121 ggtaaagaga aacgccaggt gttcttcgag atcttctcgg cggcaatggg gcgtccgccg
     3181 ccgcgcgcgc cggcgcccac ccctctttca ttcgaagaaa cctcccgggc tctcaaatct
     3241 cttacgtgcc gtgagaactc gcgaacgaga taggagccgg gagtccagac gggcagaacg
     3301 aggtcgacgg tcgacgcgcc ccgcgtgtcg atttccatcg tgatctcgta gaggtgggtc
     3361 tcgggccgcg gcatcgcaac cgtgaagcgt gtcgtgcgca tggggggatt atgagtcgag
     3421 gaacgccacg acgcgccgcg cgaacgcaac gggccgctcg gtcggaggaa cgtggccgca
     3481 gcgcggcatg acctccagcc gggcgttcgg gatcgcgcgg gccatcgcgc gccccgtcgc
     3541 gagcgggaag atgcggtcgc ggtcgcccca gaggaccagc gtctcgtgcg ggatgcgcgc
     3601 gaggtcgcgc ggcgagaagg cgtcctcccc cgccgcgagc gaggccgcga gcttcaggag
     3661 ctgcgggcgg ttcttccgcc ggcccttgta caccgccgcc gcgagcgcga cgagcgggtg
     3721 cgcgaggaag cggttgttca cgctcgtcgc gaggagccgg cgcgagtcct cgaagctgcc
     3781 gggcacgaac gaaagcgacg ccgcgagcgg gtcgatcgaa cgggccaccg gaagcccgag
     3841 ggcgtccacg agcacgagcc ggcggacgag acgcgcgtgg cggcgcacga cgtccagcgc
     3901 gacggcgccg ccgagcgagt gcccgagaag gtccacgcgc tccggcgcgt ccggcagcgc
     3961 ctcgattccg ccgcggaccg cttccgcgag gagccgcacg cggtagacga ctccggaccc
     4021 ggagggcttg tcgctcttcc cgtgcccgag gaggtcgaac gcgaggatgc gcctcttccg
     4081 cgcgagcatc gcgagggaca gggcccactc gccgtggtcg gcgccgatgc cgtgcaggag
     4141 gacgagcggc ggcgccccgc cggggcgcgt cgcgccggcg tcgagacacg cgaccgtcag
     4201 gcctcccggg atgcggaggc ggcggagcct cagtacttcc tcatcaaccc gacgacgacg
     4261 ccctggacct tcacgtcgcc ctcgggcgcg tagatcggcg ccatccggtc gttcgcgggc
     4321 tgcaggcgga cgcgcccgtc gccctcgtta tagaagcgct tcagcgtggc ttcgccgttc
     4381 acgagcgccg cgaccatctc gccgttcgcc gccttttcgc gtcggttcac gacgacgaag
     4441 tcgccgtcgt ggatgccgtc gtcgatcatc gagtggccgc tcacgcgcag gacgtagttc
     4501 tggccctttc gcgtgagcgc ctccggcacg gagacggact cgccctgggg ctcgacctcg
     4561 atgggctggc cggccgcaat gcggcccgcg agcgggatct ccaccgcgcc caccggacgg
     4621 tcttcttcgg cgagcgccag cgacctccgc ttgttccagt cgcgtaccag cacgcccttc
     4681 tcgaggagca gccggatgtg cttctggagc gtgccgaaag aggagtaccc gaactcctcg
     4741 cagatctcgc gctgcgtggg cgaaaccccg tgcttggacc ggtagcggcg gatgaagtcg
     4801 gctatttctt tttgtcgggg agtaagagta agtgccataa attattcggc tccaagatag
     4861 tttcgcctta ttttcttaga atgtgttggg tgcggatgcg cgaaccacac cggtgtggca
     4921 tcggccgtga cctgccctct tcaccttcca agaaaatctt ctttttcccg gttctttctt
     4981 ctctccgacg taagataggc gtaaatagaa tagtcgtcaa gtgtctcctt tgcgcgcaag
     5041 cggtaagatc cgggttcgtg cccgactctg cgaagccgac ccttcccgct cctcccgccc
     5101 gcggctcgtt catgcgcaaa gcgggacgct ggacgatcgg ggtcctcgcg gcggcgatcg
     5161 tcctctccgc cgccgtcctg tggctctaca accgcgcctt cacgtccgag cggcccgtcg
     5221 tgcgcgcgtc gcggctcacg tccgggaact gggtgttccc ggcgcaggtc gccgtgttcg
     5281 cggaccgcgt ggcgcgctac aagccgcgtt tcctcggcca cacggaggac tcgatcacgc
     5341 tccgccagat cgcgtccgtg aaggtgcatg ccggacccgt cttcgccgac gtcgtcatcg
     5401 acacgacggg cggttccccg ccggtcctga tccatggtct ccggaagaag gacgccgagg
     5461 cgctcagcgc ctcgatcgcg tcggcgcagt ccaccttgcc gatgccggtg aggcccgtcg
     5521 cgcccgaagc cccggccgcg ccacctcccg ccacccgctg aaccccgccg cgggcgagtc
     5581 ccgcggcgtt tcacacacga aaggaactgg aggaacccga atgaagaacg tcctgaagat
     5641 gaccgccctc gtcctctgct gcgtcgcgct cgtcgcgtgc agccagaagg gccccgccga
     5701 ggccgcgctc acggccgcca ccaccgccgt cgacggcgtg aaggccgagg ccgccaagta
     5761 cgtgcccgac caggccaaga gcctgatggc gtcgctcacg gccgcccgcg aggcgttcga
     5821 caagggcgac tacaagatgg ccctcgaggc cgccaccgcg atcccggcca aggccaagga
     5881 agccgcggcc gccgtcgccg ccaagaagga cgagctcacg aagagcttca cggcgctcac
     5941 cgcctcgctc ccggccacgg tcgagcagat caaggccaaa gtcgccgagc tcgcggcgat
     6001 gaagaagctc cccaaggaca tggacaaggc gaaggtcgaa gccgccaaga ccggcgtcga
     6061 ggccgcccag aagacgtggg ccgaggcgaa cgaggccttc aaggccggca acctcatgga
     6121 cgccgtcaaa aagggcaacg acgtcaaggg ccaggccgac cagctcctcg ccgcgctcaa
     6181 catcgcggcc aagtagccgc gcgcttccgg atcccgggac ccttcggccc gcgggcgacc
     6241 gcgggcctct ctttttcttc agctccggta gtccgcgttg caggacacgt aggcgtgccc
     6301 gaggtccgag gcgagcttga aggccgtcgc cttccccgcc ccgaggtcga cgacgaccgg
     6361 gacgcgctcc tgccggaaga ccttcgccgc gtccttctcg cggtacgcga cggggaggcc
     6421 gcggtcgacg aggacgaggc gcccgagccg gagcgagacc ttcgaggccg agaagcgcgc
     6481 cccgctgcgc cccacggccg cgaggatgcg gccccagttc gggtcgccgc cgttcagggc
     6541 cgtcttcacg agcggcgagg tggcgaccgc gtgcgccgcc gccttcgcgt cggcctccgt
     6601 gcgcgcgccg gtgatcgaga cgtcgaggac gcgcgtcgcc ccctctccgt cgcgcaccat
     6661 gagccaggcg agcttccggc agaccgccgt gacggccgcg cggagaggcg ccgcgtccgc
     6721 cgcggatgcc atctccggcc cccccatcgc tccggaggcc atgaggagca gcgtgtcgtt
     6781 cgtcgaggtg tcgccgtcca cggagatcgc gtgaaagctc cggtccgcgg cgtgcgtgag
     6841 gacggccttc aggcacgccg gcgccgccgg cgcgtccgtg gtgacgaacg cgagcatcgt
     6901 cgccatgttc gggtggatca tcccggcgcc cttcgcgatc ccgacgacgc ttccgcgcct
     6961 ccccttccac gtgaacgtcg cgcgcgcgac cttggggccc gcgtcggtcg tcatgatggc
     7021 gcgggacgcg gcgtccacgc ccgagacgga gagcttcgcc ttggcctcgg gcagcgccgc
     7081 gcggaccttc tcgtcgggca ggacgacgcc gatgacgccg gtcgaggaga cgaggacctc
     7141 gcgcttcgcg acgccgagga gcttcgcggc cgcgtcgcgc acgcgcctcg cggccgccat
     7201 cccggcctcc ccggtgacgg cgttcgcgca tccggcgttc accacgaccg cgcgcacccg
     7261 cccgccgctg gctctcaggt cggccctccc gaggacgacc ggagccgcct ggaagcggtt
     7321 cgtcgtgaaa agggccgcgg cgttcgcgcc gttctcggcg gcgagcaggg cgacgtcggg
     7381 cagcggcttc ttgcggactc cggcgtagac gccggacgcc cggaagccct tcggcagcct
     7441 cacgacgccc ctcccgagag acggcgcacc gccgcccggg ccgcgcgcgc gacctgccgc
     7501 ggtgcggtcc cgccggtgac gtcccgcttg gcgagcgcgc gccgaacgtc gagcgcctgg
     7561 aggtccttgt ccgtgatctc gtccttcggg ccgagggcga gaagggtcac gccgagcgcc
     7621 tcggcctcgg cgattcgccg gcccacgatc tcgtgcgcct
//
LOCUS       DAOMFY010000186         7625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3024_length_7625_cov_7.345443, whole genome shotgun sequence.
ACCESSION   DAOMFY010000186 DAOMFY010000000
VERSION     DAOMFY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_7625_cov_7.345443"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..91)
                     /locus_tag="PLB01_13675"
                     /pseudo
     CDS             complement(1..91)
                     /locus_tag="PLB01_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
     gene            103..240
                     /locus_tag="PLB01_13680"
     CDS             103..240
                     /locus_tag="PLB01_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78399.1"
                     /translation="MPVAGGAAPVVLRIAVAETFRPSARGDRRELGIETGAAPWLMGT
                     P"
     gene            237..1226
                     /locus_tag="PLB01_13685"
     CDS             237..1226
                     /locus_tag="PLB01_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQQ78400.1"
                     /translation="MSLLRLHPYDPRRKSRSLEIGPKGGIAFAAFALLAGGATALGLF
                     AAPRLVSDIVHAAERKAVGETAQRGAEAFASVGRRALALGSRIAGDELFLARVAAVIE
                     VPRPPGFPGDSPGTPAPTASDLESAASELARRTRVLELFRRSLAALPANEPGGFRPAA
                     VPSRSPVEPSTAVPISVFGPHESPITREPDFETGLTLASPAGSPVTATAEGTVVQVGH
                     VSRKADARWRRLGVVVILAHDARTRTVYGHLASVSVRRGQRVGRGQALAKVGTSGFAP
                     TPRVHYEVQRFENGRWVARDPRVFILDQDWIGAAELRTPPAAPEAPELPPERR"
     gene            complement(1233..2360)
                     /locus_tag="PLB01_13690"
     CDS             complement(1233..2360)
                     /locus_tag="PLB01_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQQ78401.1"
                     /translation="MDFLAEFPSKKSSIQFNHAAVCPLPARAGAALEAYAENLTTRGA
                     LDWKDWIRKADEVRALAARLVGADAPSSISIVPNTSYGLNLVALGFPFAAGDSVVTTA
                     SEFPANLAPWAALRSRGVEVRRVPTRDGAFSAADVEAACDATTRLVSVSLVAFHTGFR
                     APAEELAAFCRSRGIVFGLDGIQAAGAQRVDVAAWGVDFLSADGHKWMLGPEGAGILF
                     TTPALRERLTPPAGWTNLDRASPMDYRVPETPRFRADGVRFEPGALPTPGLYALAASL
                     GLLLEIGLPGVEARIARTLAVLVEGLPKLGFEPVLFGGPPRSGILAARPPSGKDARTY
                     QKRAAEENVVISAREGFVRFSPHVGNDEEEAERVLALLKRL"
     gene            complement(2477..3649)
                     /gene="aroC"
                     /locus_tag="PLB01_13695"
     CDS             complement(2477..3649)
                     /gene="aroC"
                     /locus_tag="PLB01_13695"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQQ78402.1"
                     /translation="MPRLTLVTAGESHGPLLTGILTGLPAGLGINPSDVNADLARRQH
                     GYGRGGRMKIERDEARFTGGLRGGVTLGGPVAFMIENRDHAVWEKVMGPLEVDAEAAA
                     KRRLSSPRPGHADLAGGLKYARRDLRDVLERASARESAARVAAGALCRQLLAAFGVRV
                     RSGVLSLGAVSVPEGPRGFDDLERVDEASPFRAVDRSVEAAMTAAVDAAAKAGDTLGG
                     TILVGARGVPAGLGSHVSWEDKLDGRIARALLSIPSVKGISLGEAVANAGRPGSEAHD
                     AILWSEARGFFRATNRAGGLEGGITNGEDVLATLYQKPIATLREGLPSVDVDTKQPHR
                     AAYERSDVTAVPACGVIGEAMLAFVLADALLEKTGGDSMEECKRNFDGFVRAQKSY"
     gene            3782..4465
                     /locus_tag="PLB01_13700"
     CDS             3782..4465
                     /locus_tag="PLB01_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQQ78403.1"
                     /translation="MRIAAVPAEDPLELIAESKLPAYGMDRNDRIVYWNDGAEKILGW
                     KPEEVLGKACFDVLAGRDVFGNVYCSHDCGVVKTASEGEEPRPFLLDVRKKGTGTVKI
                     VVRTMALPETGPRFSCLMHFIEAEGESAEPVLRALREAVNEPADGDAPRPGPVAAPEA
                     PISVSPLTAREREIVLLLSNGYAALNIAAKLNLSHATVRNHIQNVLRKLDVHSQVEAV
                     ALSFRRGWI"
     gene            4485..4886
                     /locus_tag="PLB01_13705"
     CDS             4485..4886
                     /locus_tag="PLB01_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78404.1"
                     /translation="MSRVVSRAAAIRMLRDAFLARTDQEHSMCDVASRDRIFCGGFRA
                     WSDEELRKRFSTIEARRPGLPRVAFEELANKWQLGRQIVHGSPLACDVQTKEHGTCRG
                     WDAFSNADLERYCAEVLGETLVVTETGAAEA"
     gene            complement(4896..5840)
                     /locus_tag="PLB01_13710"
     CDS             complement(4896..5840)
                     /locus_tag="PLB01_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HQQ78405.1"
                     /translation="MDLSSSYLGLRLANPVVPGASPLTGDLDTIRRLEDAGAPAVVLP
                     SLFEEQVAAEALGHEGALTDPDRYLRQVRAAKAAVSIPVIASLNGTTDEGWMSFARLV
                     EQAGADALELNLFHISTNLWASGATAEAHLLESVAAARRSISIPVAVKIFPYFSSIAS
                     LARQLEEVGANGLVLFNRLYQPDIDLEGGEPTADPRLSSPSELVSRLHWIALLAGRVD
                     VSLAASGGVHTAADVLRCVLAGADVTQMVSALILHGVAHLRTVTDGIARWLEAHDHES
                     LDDVRGLMSLERRADPRAFPRPGYLQVLQGWKTAERRG"
     gene            complement(5925..7379)
                     /locus_tag="PLB01_13715"
     CDS             complement(5925..7379)
                     /locus_tag="PLB01_13715"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HQQ78406.1"
                     /translation="MTPEPAGLAARWMRPILFLGRNRLSIFGAVLTTGAVFSLVGFWI
                     IEANASKPLHPYAGIVFILVLPAIFVLGLVLIPIGILFERRRLKAEGALPSVYPRIDL
                     TDPGLRRFGSLVALLSIVNVIILTFSAYLGVEYMESSRFCGLTCHKVMSPEYGAYLDS
                     PHSRVSCTSCHIGPGASWAVKAKVDGVRQVVAVVFGTYSRPIPSPVHTLRPARATCEQ
                     CHWPQKFSGDKLRIVTKYAEDEKNTESKTVLLLKLGGRRAAGAVGIHGRHLDDKERIS
                     YTTADGRREVIPTVNYLDDDGKTVVFTSSEVKPPEPGKGRSETRTMDCIDCHNRPTHA
                     YQLPDRAVNEAMSGGAISTELPFVRKKAVEVLKAEYASREVAFDRISKDLTEFYKAKY
                     PEVYASRRASIETAVREIQRIYGRNVFPEMRLTWGTHPNHIGHEDFLGCFRCHDGNHK
                     APGGRVISQECDACHQILAQDETNPKVLADLGIK"
     gene            complement(7404..>7625)
                     /locus_tag="PLB01_13720"
     CDS             complement(7404..>7625)
                     /locus_tag="PLB01_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HQQ78407.1"
                     /translation="PMDTVIFTGVLPLEEVKHERPAEYERLIAEGRVEAIVAPAPTPQ
                     EFRRGRMIGTIAVCVGLLLVSLTIYALLS"
BASE COUNT         1119 a         2723 c         2683 g         1100 t
ORIGIN      
        1 cgacggcggc ctcgtacgag atgttctcgt cgtggtcgcc cgcctcggcc ttggaggccg
       61 gggtgaagat gggcgccggc aggcggctgg cggcgcctcg aggtgcccgt cgcgggcggc
      121 gcggcgccgg tcgtcctgcg catcgccgtc gccgagacgt tccggccatc cgcgcgcggc
      181 gaccgccgcg agctcggcat cgaaacgggc gccgcaccgt ggctgatggg gacgccgtga
      241 gcctcctgcg cctccatccc tacgacccac gccggaagag ccggtccctc gagatcgggc
      301 cgaagggagg aatcgcgttc gcggccttcg cgctcctggc cggaggcgcg acggccctcg
      361 ggctcttcgc cgccccgcgc ctcgtctccg acatcgtcca cgcggccgag cggaaggccg
      421 tcggcgagac cgcgcagcgg ggcgcggagg ccttcgcgtc ggtcggccgg cgcgccctcg
      481 ccctcggaag ccgcatcgcc ggagacgagc tgttcctcgc gcgcgtcgcc gccgtgatcg
      541 aggtgcctcg tcccccgggc tttcccggcg actcgcccgg gactcccgcg cctaccgcgt
      601 ccgacctcga gagcgcggcg tccgagctcg cacgccgcac gcgcgtcctc gagctcttcc
      661 ggcgcagcct cgcggcgctg ccggcgaacg agccgggggg cttccggccc gcggccgtgc
      721 cctcccgttc cccggtcgag ccctcgacgg ccgtgcccat ctcggtcttc gggccgcacg
      781 agtcccccat cacgcgcgag cccgacttcg agaccggact cactctggcc tctccggcgg
      841 ggagccccgt gacggccacg gcagagggta cggtcgtgca ggtgggtcat gtgtcgcgca
      901 aggccgacgc gcgctggcgg cgcctcggcg tcgtcgtcat cctcgcgcac gacgcgagga
      961 cgcgcaccgt ctacggccac ctcgcgagcg tctcggtgcg ccgcggccag cgcgtcggga
     1021 ggggacaggc gctcgcgaag gtcgggacga gcggtttcgc gccgacgccg cgcgtccact
     1081 acgaggtcca gcgtttcgag aacggccggt gggtcgcacg cgacccgcgc gtcttcatcc
     1141 tcgaccagga ctggatcggc gccgccgagc tgcgcacgcc cccggcggcg cccgaagcac
     1201 ccgagcttcc gcccgagcgc cgctgacccg agctacagcc tcttcagcag cgcgagcacg
     1261 cgctccgcct cctcctcgtc gtttccgacg tggggagaga agcgcacgaa gccctcgcgc
     1321 gccgagatga cgacgttctc ctcggccgcg cgcttctggt aggtgcgcgc gtccttcccc
     1381 gacggcggcc gtgcggcgag gatgcccgag cgcggcggcc ccccgaagag gaccggttcg
     1441 aacccgagct tcggaagccc ctcgacgagg acggcgagcg tccgcgcgat tcgcgcctcg
     1501 accccgggca gcccgatctc gaggaggaga ccgagcgagg ccgccagcgc gtacaggccc
     1561 ggcgtcggaa gcgcgcccgg ctcgaagcgg acgccgtcgg cacggaagcg cggcgtctcg
     1621 ggcacccggt agtccatcgg tgaggcgcga tcgaggttcg tccagcccgc cggcggcgtc
     1681 aggcgctcgc gcagcgccgg cgtcgtgaag aggattcccg cgccctcggg ccccagcatc
     1741 cacttgtggc cgtccgccga gaggaagtcg actccccagg ccgcgacgtc gacgcgctgt
     1801 gcgccggcgg cctggatccc gtcgaggccg aagacgatgc cgcgcgagcg gcagaacgcg
     1861 gcgagctcct cggccggcgc gcggaagccc gtgtggaacg cgacgagtga gaccgagacg
     1921 aggcgcgtcg tcgcgtcgca ggccgcctcg acgtcggcgg cggagaacgc cccgtcgcgc
     1981 gtcgggacgc gccggacctc gacgccgcgg gaacgaagtg ccgcccatgg cgcgaggttc
     2041 gccgggaact ccgaggccgt cgtcacgacc gaatcacccg cggcgaacgg gaaccccaag
     2101 gcgacgaggt tcagtccgta cgacgtgttc gggacgatcg agatggagga gggcgcgtcg
     2161 gcgccgacga ggcgtgccgc gagcgcccgc acctcgtcgg ccttgcggat ccaatccttc
     2221 cagtcgagcg cgccgcgggt ggtgaggttc tcggcgtatg cctcaagagc cgcacccgct
     2281 cgcgcgggca gcggacaaac agccgcatgg ttgaattgaa tggaagattt cttagaaggg
     2341 aattcggcca ggaagtccaa tcaaccctct gtcgcgcgcg aggcgcctct tcacccttct
     2401 aagaaaatct tctctcttct cttctcttct cttctcttct cttctcttct cttctcttct
     2461 cttctcttct tcccactcaa tagctcttct gcgcccgaac gaacccgtcg aagttccgct
     2521 tgcattcttc catcgaatcc ccgcccgtct tctcgagcag cgcgtccgcg agaacgaacg
     2581 ccagcatcgc ctcgccgatg acgccgcagg cgggcacggc cgtgacgtcc gagcgttcgt
     2641 acgccgcgcg gtgcggctgc ttcgtgtcga cgtccacgga cggcagtccc tcgcgcagcg
     2701 tcgcgatcgg cttctggtag agcgtcgcga ggacgtcctc gccgttcgtg atcccgccct
     2761 ccaggccgcc cgcgcggttc gtcgcccgga agaagccgcg cgcctcgctc cagaggatcg
     2821 cgtcgtgcgc ctcggagccg ggccggcccg cgttcgcgac ggcctcgccg agcgagatgc
     2881 ccttcaccga cgggatcgac aggagcgccc gcgcgatccg gccgtcgagc ttgtcctccc
     2941 acgagacgtg cgagccgagc cccgccggca cgccgcgggc gccgacgagg atggtcccgc
     3001 ccagcgtgtc gcccgccttc gcggccgcgt ccacggcggc ggtcatcgcc gcctcgacgc
     3061 tccggtccac ggcgcggaac ggcgaggcct cgtccacgcg ttcgaggtcg tcgaagccgc
     3121 gcggaccctc cgggaccgac acggcgccga gggacaggac gccgctccgg acgcggacgc
     3181 cgaacgcggc gaggagctgc cggcagagcg ccccggcggc cacgcgtgcc gcggattccc
     3241 gggcgctcgc gcgctcgagg acgtctcgaa ggtcgcgccg ggcgtacttg aggccccccg
     3301 cgaggtcggc gtggccgggc cgcggcgacg aaagtcggcg cttcgcggcc gcctcggcat
     3361 cgacctcgag cggtcccatg accttttccc agacggcgtg gtcgcggttc tcgatcatga
     3421 acgcgaccgg ccctccgagc gtcaccccgc cccgaaggcc ccccgtgaag cgggcctcgt
     3481 cgcgctcgat cttcatccgg ccgccgcgcc cgtagccgtg ctggcggcgc gcgaggtcgg
     3541 cattaacgtc cgacggattg attcccagtc ccgccgggag gcccgtgagg atcccggtca
     3601 ggagcgggcc gtgggactcc ccggcggtca cgagggtcag gcgcggcatg cggcgagtct
     3661 accggttgcg ggtgcggtag actccccgac gccccggccc cggtccgaga ctcaggctcg
     3721 aacgcatgcg gggagaaggg gcaggggcca tcacatttct gacagaatcg gggtccgacc
     3781 gatgagaatc gccgcagtgc ccgcagaaga tcccctcgaa ctgatcgccg agtcgaagct
     3841 cccggcctac ggaatggacc ggaacgaccg gatcgtctac tggaacgacg gcgccgaaaa
     3901 gatcctcggg tggaagcccg aagaggtgct cggaaaggcg tgcttcgacg tcctcgcggg
     3961 tcgcgacgtc ttcggaaacg tctactgctc gcacgactgc ggcgtcgtca agacggcttc
     4021 cgagggcgag gagccgcgtc ccttcctgct cgacgtccgg aagaagggga cgggcaccgt
     4081 gaagatcgtg gtccgcacga tggcgctccc cgagacgggc ccgcgcttct cgtgcctgat
     4141 gcacttcatc gaggccgagg gagagtcggc cgaaccggtc ctcagggcgc tccgcgaggc
     4201 ggtgaacgag cccgcggacg gcgacgcccc gcgcccggga cccgtcgcgg cgcccgaggc
     4261 tccgatctcc gtctcgccgc tcacggcccg cgagcgcgag atcgtcctcc tgctctcgaa
     4321 cggctacgcg gccctcaaca tcgccgcgaa gctgaacctc tcgcacgcca ccgtgcggaa
     4381 ccacatccag aacgtgctcc gcaagctcga cgtccacagc caggtcgagg ccgtcgcgct
     4441 ctccttccgc cgcggctgga tctgacggag cgcgcgaaac tcccgtgagc cgcgtcgtct
     4501 cgagggccgc cgcgatccgg atgctgcgcg acgccttcct cgcgcgcacg gaccaggagc
     4561 actcgatgtg cgacgtcgcc tcgcgcgacc ggatcttctg cggcggcttc cgcgcgtggt
     4621 ccgacgagga gctgcgaaag cgcttctcca cgatcgaggc gcgccgtccc ggactccccc
     4681 gtgtcgcttt cgaggagctc gcgaacaagt ggcagctcgg ccgccagatc gtccacggct
     4741 cgccgctcgc gtgcgacgtc cagacgaagg agcacggcac ttgccggggc tgggacgcgt
     4801 tctcgaacgc cgacctcgag cgctattgcg cggaggtcct cggcgagacg ctcgtcgtca
     4861 ccgagaccgg cgccgccgaa gcctgagccg ggggctcagc cccgcctctc ggcggtcttc
     4921 cacccctgga gcacctgcag atagcccggc cgcgggaacg cgcgcgggtc ggcccgccgc
     4981 tcgagactca tgagcccgcg gacgtcgtcg agcgactcgt ggtcgtgcgc ctcgagccac
     5041 cgggcgatcc cgtccgtcac cgtcctgagg tgcgcgacgc cgtggaggat gagggccgag
     5101 accatctggg tgacgtcggc acccgcgagg acgcagcgga ggacgtcggc cgccgtatgg
     5161 acgcctcccg aagcggcgag ggaaacgtcg actcgccccg cgaggagcgc gatccagtgg
     5221 aggcgcgaga cgagctcgga cgggctcgag aggcgcgggt cggccgtcgg ctctccgccc
     5281 tcgaggtcga tgtccggctg gtagagccgg ttgaagagga cgaggccgtt cgccccgacc
     5341 tcctcgagct gtcgcgcgag cgaggcgatc gacgaaaagt aggggaagat cttcacggcg
     5401 acgggaatcg agatgctgcg ccgcgccgcc gcgacgctct cgaggaggtg cgcctcggca
     5461 gtcgccccgc tcgcccagag gttcgtcgag atgtgaaaca gattgagctc gagggcgtcg
     5521 gcgcccgcct gctcgacgag ccgcgcgaag gacatccagc cctcgtccgt cgtcccgttc
     5581 agggacgcga tcacggggat cgacacggcg gccttggcgg cccggacctg cctcaggtaa
     5641 cggtcggggt ccgtcagggc gccctcgtga cccagggcct cggccgcgac ctgttcctcg
     5701 aacagcgacg ggaggacgac cgcgggcgcc ccggcgtcct cgagccgacg gatcgtgtcg
     5761 aggtcgccgg tcagcgggga ggcgccgggg acgacgggat tcgcgaggcg aaggccgagg
     5821 taagacgacg agagatccac tctctcgaag gtcgcccgaa cggccggggg acgccatgat
     5881 gcagaggatt caggcgtccc cggccccccg gtctcttcct cgtcctactt gatccccagg
     5941 tccgcgagga ccttcgggtt cgtctcgtcc tgcgccagga tctggtggca cgcgtcgcac
     6001 tcctgcgaaa tcacgcgccc gccgggagcc ttgtggttgc cgtcgtggca gcggaagcac
     6061 ccgaggaagt cctcgtggcc gatgtggttc gggtgcgtgc cccacgtcag gcgcatctcg
     6121 ggaaagacgt tccggccgta gatccgctgg atctcgcgca cggccgtctc gatggacgcg
     6181 cggcgggacg cgtagacctc gggatacttg gccttgtaga actccgtgag gtccttggag
     6241 atgcggtcga aggcgacctc gcgtgaggcg tactcggcct tgaggacctc gacggccttc
     6301 ttgcggacga acggcagctc cgtgctgatc gcgccgcccg acatcgcctc gttcacggcg
     6361 cggtccggca gctggtaggc gtgcgtcgga cggttgtggc agtcgatgca gtccatcgtc
     6421 cgcgtttcgg acctgccttt tccgggctcg gggggcttca cctcgctcga cgtgaagacg
     6481 accgtcttcc cgtcgtcgtc gaggtagttc acggtcggga tgacctcgcg gcggccgtcc
     6541 gccgtcgtgt aggagatgcg ctccttgtcg tcgaggtggc ggccgtggat tccgaccgcg
     6601 ccggccgccc tccggccccc gagcttgagg aggaggaccg tcttggactc ggtgttcttc
     6661 tcgtcttcgg cgtacttcgt gacgatccgg agcttgtcgc ccgagaactt ctgcggccag
     6721 tgacactgct cgcaggtggc gcgcgccgga cgcagcgtgt ggacgggcga ggggatcggg
     6781 cgcgagtagg tcccgaacac gaccgcgacg acctgacgca cgccgtcgac cttcgccttg
     6841 acggcccacg aggcccccgg tccgatgtga cagctcgtgc aggagacgcg cgagtgcggc
     6901 gagtccaggt acgcgccgta ctcgggcgac atgaccttgt ggcacgtcag gccgcagaac
     6961 cgcgaggact ccatgtactc gacgcccagg tacgccgaga acgtcaggat gatgacgttc
     7021 acgatcgaga gcagcgcgac gagggagccg aaccggcgca ggccggggtc cgtgaggtcg
     7081 atcctcgggt aaacggacgg cagcgcccct tccgccttca ggcggcggcg ctcgaacagg
     7141 atcccgatcg ggatcaggac gaggcccagc acgaagatgg ccggcaggac gagaatgaag
     7201 acgatgcccg cgtagggatg gaggggcttc gaggcgttcg cctcgatgat ccagaaaccg
     7261 accagggaga aaacggcgcc cgtcgtcagg acggcgccga agatggagag gcggttgcgg
     7321 ccgagaaaca gaatcggccg catccaccgg gccgcgaggc ccgcgggctc tggggtcatg
     7381 gactcctcct gaggttcctg gaatcagctc aacagagcat agatcgtcaa ggatacgaga
     7441 aggagcccga cgcagaccgc gatcgtgccg atcatccggc cgcggcgaaa ttcctgcggc
     7501 gtcggcgccg gggccacgat cgcctcgacc cggccttccg cgatcaggcg ctcgtactcg
     7561 gcggggcgct cgtgcttcac ctcctcgagc ggcaggacgc ccgtgaagat caccgtgtcc
     7621 atcgg
//
LOCUS       DAOMFY010000187         7603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3038_length_7603_cov_8.846184, whole genome shotgun sequence.
ACCESSION   DAOMFY010000187 DAOMFY010000000
VERSION     DAOMFY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_7603_cov_8.846184"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..405)
                     /locus_tag="PLB01_13725"
     CDS             complement(172..405)
                     /locus_tag="PLB01_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQQ78408.1"
                     /translation="MTCHDVHAFLSDWLTGDLPRATREVFARHLGACASCRAYVDSYE
                     RTRDLAKLAEREAEGRVPEALVRAILAARAGAI"
     gene            complement(402..914)
                     /locus_tag="PLB01_13730"
     CDS             complement(402..914)
                     /locus_tag="PLB01_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQQ78409.1"
                     /translation="MIADLYLKLTARAEKTFAPFGSLVLLVLRLVWGWQFLTTGLGKL
                     QNHARVTDFFTSLGIPLPGLNAWFVGGLEMVGGALLLLGLFSRPIALLLTGNMFVAYL
                     TGDRPALLGVFHDLDAFLKADPFWFLFVSVVVLTLGPGAISLDRLLAKVISGKRERDA
                     QAVRLGAAVA"
     gene            complement(911..2095)
                     /locus_tag="PLB01_13735"
     CDS             complement(911..2095)
                     /locus_tag="PLB01_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ78410.1"
                     /translation="MKTISLFRTRAGMQAPEHRRRSANFFEGLIAAVVLGAAIALDIA
                     AMAQAPPAASPLRSSPSKKSSSSVSSDVAAPLVSAVVSVGMTVSDLDRSVDWFTRVLT
                     FSKVSEAEASGPEVERLYGVFGARVRVATLRLGGETLELTEFVAPKGRPVPPDARSND
                     RSFQHVAIVVSDMAKAYARLRAAGAVHASTSPQKLPAWNPNAGGIEAFYFRDPDGHAL
                     EIIRFPEGKGDPRWQSAPSGALFLGIDHTAIVVADTDASRRFYAGLLGLKVAGESENW
                     GEEQEHLNAVFGARLRITGLRAASGPGVEFLEYLVPADGRPAPSDSRANDLWHWQTRV
                     AVADAAAAERVLFAARARFVSTGARSFPDRPLDFTRALLVRDPDGHALELTENSSQGD
                     RP"
     gene            complement(2092..3690)
                     /locus_tag="PLB01_13740"
     CDS             complement(2092..3690)
                     /locus_tag="PLB01_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQQ78411.1"
                     /translation="MPGTRYDVIIIGTGAGGGTLLSALAPSGKKILVLERGDFVKRER
                     ENWDPRAVNVEGRYAAKDVWKDKTGADLHPHTNYAVGGNTKFYGAALFRLRREDFGVL
                     PHHGGVSPAWPISYDDLEPYYTRAERQYFVHGTRGEDPTEPPASAPYPHPAVSHEPRL
                     QALAEDFAAQGARPFHTPLGIRLDEGSRRSLCIRCATCDGFPCLVNAKSDAHTCAVEP
                     ALERPNVTLLVNAKVVKLETSPSGREVTAVHVEREGVRETYAADVVVLSAGAINSAAL
                     LLASANEKHPRGLANGSDVVGRHYMGHVNSVLMAVSKCPNPTVFQKSLSLNDYYFGDA
                     DFRFPMGHISFVGKLDGVTLSAGAPALAPGFTLDLMAKHSLDFWLTSEDLPDPENRVT
                     LDRDGKIVLTYLPNNQEGHRQLHKRLKALVQGQRACVVHGKDCHAGLFGRSLFVGQQI
                     PLAGVAHQNGTIRFGTSPKSSALDVNCKAHELDNLYVVDGSFFPSSAAVNPALTIMAN
                     ALRVGDHLLERLGAKAGVEAGVAA"
     gene            complement(3750..4544)
                     /locus_tag="PLB01_13745"
     CDS             complement(3750..4544)
                     /locus_tag="PLB01_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HQQ78412.1"
                     /translation="MSAGIEPAMRDALKARWPELLIEGTLLGLFMLSASVFCVLLEHP
                     SSAVRAALPDANVRRALMGLAMGATAVSLIYSPWGKRSGAHMNPAVTLTFWRLGKVKT
                     PDAVLYAAAQLAGGLVGMGVAVLLLNERVANPAVHFVVTAPGARGPLPAFLAEVFISF
                     VLMTTVLASSARPRLAPFTGIFCSSLIFVFITFEAPVSGMSMNPARTLASAVPARDFM
                     SLWVYLVAPPLGMLMAVEARRAGGAAAAAFCARLSPHGRARCPFCG"
     gene            complement(4554..6512)
                     /locus_tag="PLB01_13750"
     CDS             complement(4554..6512)
                     /locus_tag="PLB01_13750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HQQ78413.1"
                     /translation="MTAETRDSGEWLETNGLGGYAMSTATGENTRRYHGLLVAATRPP
                     AGRSVLLSKLEETLVLAGRRVDLSTNRFPGTLHPEGWRLLTGFRMEPFPTFTWEVDGV
                     VVEKEVFLPHGENTVCVTWRIVSAPKDAGAIGLEVRPLVAFRDFHALTHENAELDRRV
                     EIEEGRIRVAPYRNLPELFVAHGGAATRAGDWYRSFEYEEEQARGFDYVEDLWHPATL
                     LFTFEEIPSSSGPRARREATVIASTSPRSASDLHLLRKNELTRREKLTGNAATDPFVR
                     ALSRASDRFLVARGGGSSVIAGYPWFSDWGRDTMIALPGLTLATGRPEIARGILLEFA
                     EHASEGMLPNRFPDTGGGDGAPEYNTVDGTLWYFEAIRAYAAATGDLDLVRKLYPVLA
                     DILAWHLRGTRYGIRVDDDGLLEWHAAGEALTWMDARVDGRPVTPRQGKPVEIQALWF
                     NALCTMQEFARKIGDQISSNECAARAAACRASFLNLFSDGAGGLADVVDDHSKDSSIR
                     PNQILAVSLHYSMVGKPLARRILATIEEHLLTPMGLRTLSPRDPAYRGRYEGGPAERD
                     AAYHQGTVWPWLLGPYVEALLKTHGRGPASLARARAVLAPLRSFVLGGGLGQIPEIFD
                     GDEPRLPRGCPAQAWSVSEVLRAALLAA"
     gene            complement(6526..>7603)
                     /locus_tag="PLB01_13755"
     CDS             complement(6526..>7603)
                     /locus_tag="PLB01_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78414.1"
                     /translation="APLGLVLGLPGVGRPGTPAGVSPLRAVGVGSLAGVLAATVFEIW
                     MASGEFFPLLAGLGEAGRSPAGLSVHFLFAVAIGSLFGLLFQRDLYGLGSSMGWGVGY
                     GILCWFVFPLTLFPLLSGMPLDWSADHAGDLFGPLVGYVLYGVIVGIVFAVVERLRVR
                     LFVETDPIRREPEGPGVQTLRTAGWGALAGLGGGIVAAPILVATGVLPKIAGVEGGLS
                     LSRGLVLHLFASALLGVLWGFLFRREGLGLGRGLSWGLVFGVVWWYVGPLTLLPLLAT
                     GEIDWRPSAASALLPSLVGHLVFGALTAVIFVAQQARQSRWLLLDPRHAAREKRRTRP
                     SGTPAPALWLFVLGLGVLLPILLG"
BASE COUNT         1243 a         2485 c         2850 g         1025 t
ORIGIN      
        1 ctccttgtct tccttggaga acatcagccg gccggcggag ccgctggagc tgccaccgca
       61 agcggcgagg gcgagagcga gagaaaacag gaagcgtttg ctgcgggaca tcgaatcctc
      121 catcggtgga ttcgatgcct ccctcggcgc gggggtttcg agagggagat cctagatcgc
      181 gccggcgcgg gccgcgagga tggcgcgcac cagcgcctcc gggacgcggc cctcggcctc
      241 gcgctcggcg agcttcgcga ggtctcgggt tctctcgtac gagtccacgt acgcacggca
      301 gctcgcgcat gcgccgagat ggcgcgcgaa gacctcccgc gtcgcgcgcg ggaggtcgcc
      361 cgtcagccag tccgacagga aggcgtggac gtcgtgacag gtcacgcgac cgcggctccg
      421 agccgcacgg cctgggcgtc gcgctcgcgc tttccgctga tgaccttcgc caggaggcga
      481 tcgagcgaga tcgcgccggg gcccagggtc aggacgacga ccgagacgaa gaggaaccag
      541 aacgggtcgg ccttcaggaa ggcgtcgagg tcgtggaaga cgccgaggag ggccgggcgg
      601 tctccggtca ggtacgccac gaacatgttc cccgtgagga ggagcgcgat cgggcgcgaa
      661 aagaggccga ggaggaggag cgcccctccc accatctcga ggccgccgac gaaccaggcg
      721 ttcaggccgg gcaagggaat cccgagcgac gtgaagaagt ccgtcacgcg cgcgtgattc
      781 tggagtttgc cgaggcccgt cgtgaggaac tgccatcccc agacgagcct gaggacgagg
      841 aggacgaggc ttccgaaggg cgcgaacgtc ttctcggccc gcgcggtgag cttgaggtag
      901 aggtcggcga tcatgggcgg tctccttgag atgaattctc cgtcagctcg agcgcgtggc
      961 cgtccggatc gcggacgagg agcgcacggg tgaagtcgag agggcggtcg gggaaggagc
     1021 gggcgcccgt cgagacgaag cgcgcgcggg cggcgaagag cacccgttcg gcggcggccg
     1081 cgtcggcgac ggccacgcgc gtctgccagt gccagaggtc gttcgcgcgg ctgtccgagg
     1141 gagcggggcg gccgtcggca ggcacgaggt actcgaggaa ctcgacgccg ggcccggagg
     1201 ccgccctgag gccggtgatc cggaggcgcg cgccgaagac ggcgttgagg tgctcctgct
     1261 cctcgcccca gttctcgctc tccccggcga ccttcagccc gagaaggccg gcatagaagc
     1321 ggcggctcgc gtccgtgtcg gcgacgacga tcgccgtgtg gtcgatgccg aggaagagcg
     1381 cacccgacgg cgcgctctgc cacctcggat cgcccttacc ctcggggaac cggatgatct
     1441 cgagggcgtg gccgtcgggg tcccggaagt agaacgcctc gatgccgccc gcgttcgggt
     1501 tccaggccgg gagcttctgc ggcgacgtcg aggcgtggac cgcgcccgcg gcgcgcaggc
     1561 gcgcgtatgc cttcgccatg tcggagacga cgatcgccac gtgctggaac gagcggtcgt
     1621 tcgaccgggc gtcgggcggc acgggccgtc ccttcggcgc gacgaactcc gtgagctcga
     1681 gcgtctcgcc gccgaggcgc agagtcgcga cgcgcacacg cgccccgaag acgccgtaga
     1741 gccgctcgac ctcggggccg gacgcctccg cttcggagac cttcgagaac gtcaggacgc
     1801 gcgtgaacca gtcgacggac cggtcgagat cggagaccgt catgccgacg gagacgacgg
     1861 ccgagacgag cggcgccgcg acgtccgaag agaccgaaga ggaagatttc ttagaagggg
     1921 aagagcggag gggcgacgcg gcaggtggtg cctgcgccat tgcggcgatg tcgagggcga
     1981 tggcggcgcc cagaacgacg gcggcaatca acccttcgaa gaaatttgca gaccggcgcc
     2041 tgtgctccgg cgcctgcatt cctgctcggg tccgaaacag agagattgtc ttcacgccgc
     2101 gacccccgcc tcgacgccgg ccttcgcacc gagccgctcg agaaggtggt cgcccacccg
     2161 gagcgcattc gccatgatcg tgagcgcggg attcaccgcg gcgctcgacg ggaagaagct
     2221 gccgtcgacg acgtagaggt tgtcgagctc gtgggccttg cagttcacgt ccagggccga
     2281 cgacttcgga ctcgtcccga accggatcgt cccgttctgg tgcgcgacgc ccgcgagcgg
     2341 gatctgctgg ccgacgaaga ggctgcgccc gaagagcccg gcgtggcagt ccttcccgtg
     2401 caccacgcac gcgcgctggc cctggacgag cgccttgagc cgcttgtgca gctgccggtg
     2461 cccttcctgg ttgttcggga ggtacgtgag gacgatcttc ccgtcccggt cgagcgtgac
     2521 gcggttctcg ggatccggga ggtcctcgga cgtgagccag aagtcgagcg agtgcttcgc
     2581 catgaggtcg agcgtgaagc ccggcgcaag cgcgggcgct cccgccgaca gcgtcacgcc
     2641 gtccagcttg ccgacgaacg agatgtggcc catcgggaag cggaagtccg cgtcgccgaa
     2701 gtagtagtcg ttgagggaca ggctcttctg gaagaccgtc gggttcgggc acttcgagac
     2761 cgccatgagg accgagttca cgtgtcccat gtagtggcgt ccgacgacgt ccgagccgtt
     2821 cgccagaccg cgcgggtgct tctcgttcgc cgaggcgagg aggagcgccg cggagttgat
     2881 cgcgccggcg gagagcacga cgacgtcggc ggcgtacgtt tcgcggacgc cctcgcgttc
     2941 gacgtgtacc gccgtgacct cgcgccccga cgggctcgtc tcgagcttca cgaccttcgc
     3001 gttcacgagg agcgtcacgt tcggccgctc gagcgcgggc tcgacggcgc acgtgtgggc
     3061 gtcggacttg gcgttcacga ggcacgggaa gccgtcgcac gtcgcgcagc ggatgcagag
     3121 gctcctccgg ctcccctcgt cgaggcggat cccgaggggc gtgtggaacg gtcgggcgcc
     3181 ctgcgccgcg aagtcctcgg cgagggcctg aagacgcggc tcgtgcgaga ccgccgggtg
     3241 cgggtacggt gcggacgcgg gcggctcggt cgggtcttcc ccgcgtgtgc cgtggacgaa
     3301 gtactggcgc tcggcgcgcg tgtagtacgg ctcgaggtcg tcgtacgaga tcggccacgc
     3361 gggcgagacg cctccgtggt gggggaggac gccgaagtcc tccctgcgga ggcggaagag
     3421 cgccgcgccg tagaacttgg tgttcccgcc gacggcgtag ttcgtgtgcg gatgcaggtc
     3481 ggcgccggtc ttgtccttcc agacgtcctt cgcggcgtag cggccctcga cgttcacggc
     3541 gcgcgggtcc cagttttccc gctcgcgctt cacgaagtcg ccgcgttcga ggacgaggat
     3601 cttcttgccg gagggagcga gggccgagag gagcgtgccc cctccggccc ccgtcccgat
     3661 gatgatcacg tcgtagcgcg tccccggcat tcctcactcc cttcgatggc gttcggaccc
     3721 tccttggatg ccggcagggc ccggaggttt cagccgcaga aggggcagcg ggcgcggccg
     3781 tgaggggaga ggcgggcgca gaacgccgct gccgccgccc cgccggcccg gcgggcctcg
     3841 acggccatca gcatcccgag gggcggcgcg acgaggtaga cccagagaga catgaaatcg
     3901 cgggcgggca cggccgaggc gagcgtgcgc gcgggattca tcgacatccc ggagacggga
     3961 gcctcgaacg tgatgaagac gaagatgaga gacgaacaga agattcctgt gaaaggggcg
     4021 agacggggcc gtgcggagct cgcgaggacg gtcgtcatga ggacgaagga gatgaagact
     4081 tctgccagga aggcggggag gggccctcgg gcccccggcg cggtcacgac gaaatggacg
     4141 gccggattcg cgactctttc attcagaaga aggacggcca cgcccatacc gacgagcccg
     4201 cctgcgagct gcgccgccgc gtaaaggacc gcgtcgggcg tcttcacctt ccccagccgc
     4261 cagaacgtca gcgtgacggc cgggttcatg tgcgcgcccg atctctttcc ccacggggag
     4321 tagatgagcg aaaccgcggt cgcccccatc gcgaggccca tcagcgcgcg gcggacgttc
     4381 gcgtccggca gcgcggcgcg caccgccgag gaggggtgct cgaggaggac gcagaagacc
     4441 gacgccgaca gcatgaagag accgagaagc gtcccttcga tgaggagctc cggccagcgg
     4501 gctttcagcg cgtcgcgcat cgccggttcg atgccggcgg acacttctcc gtttcaggcc
     4561 gcgaggagag ccgcgcggag gacctcggac acgctccacg cctgcgcggg gcagccccgc
     4621 gggaggcgcg gctcgtcgcc gtcgaagatc tcgggaatct gcccgaggcc gccgccgagg
     4681 acgaagctgc gcagcggcgc gaggacggcg cgcgcgcgcg cgagcgacgc cggaccgcgc
     4741 ccgtgcgtct tcaggagcgc ctcgacgtac gggccgagga gccacggcca gacggtgcct
     4801 tggtggtagg ccgcgtcgcg ctcggcgggc cccccctcgt accggccgcg gtacgcgggg
     4861 tcgcgggggg agagcgtccg gagacccatc ggcgtcagga ggtgctcctc gatggtcgcg
     4921 aggatgcggc gcgcgagcgg cttgccgacc atcgagtagt ggaggctcac ggcgaggatc
     4981 tgattcgggc ggatcgaaga gtccttcgaa tggtcatcga cgacgtctgc caggccccct
     5041 gcgccgtcgg aaaagagatt cagaaagctc gcgcggcacg ccgcagctct ggccgcacat
     5101 tcattcgaag aaatctggtc tccgatcttc ctcgcaaatt cctgcatcgt gcagagcgcg
     5161 ttgaaccaga gcgcctggat ctcgaccggt ttgccctgcc ggggcgtgac ggggcggccg
     5221 tcgacgcgcg cgtccatcca cgtgagggcc tcgccggccg cgtgccattc gaggaggccg
     5281 tcgtcgtcca cgcggatgcc gtaacgcgtg ccccgcaggt gccacgcgag gatgtcggcg
     5341 aggacggggt agagtttcct gacgaggtcg aggtctcccg tggccgccgc ataggcccgg
     5401 atcgcctcga agtaccagag cgtcccgtcg acggtgttgt attcgggagc gccgtctcct
     5461 ccgcccgtgt ccggaaaccg gttgggcagc atgccctcgc tcgcgtgctc tgcgaattcg
     5521 aggaggatgc ccctcgcgat ctcgggacgg cccgtcgcga gcgtgaggcc cggcagggcg
     5581 atcatcgtgt cgcggcccca gtccgagaac cacgggtagc cggcgatgac gctgctcccg
     5641 cccccccggg cgaccaggaa ccggtccgag gcgcgcgaga gcgcgcggac aaagggatcg
     5701 gtcgcggcgt ttccggtgag cttctcgcgg cgggtgagct cgttctttct tagaaggtga
     5761 agatcggacg cggagcgggg cgaggtcgag gcgatgacgg tcgcctcgcg gcgcgcccga
     5821 ggaccggagg aggaggggat ttcttcgaag gtgaagagca gcgtggcggg gtgccacagg
     5881 tcttccacgt agtcgaagcc gcgcgcctgc tcttcctcat attcgaagga tctgtaccag
     5941 tcgcccgccc gcgtggctgc cccgccgtgc gccacgaaga gttcggggag gtttctgtat
     6001 ggagcaaccc ggattcgccc ctcctcgatc tcgactctcc gatcgagctc cgcgttctcg
     6061 tgcgtcagcg cgtggaagtc gcggaaggcg acgagcggcc ggacctcgag gccgatcgct
     6121 ccggcgtcct tcggggcgct cacgatccgc cacgtcacgc agacggtgtt ctcgccgtgc
     6181 ggaaggaaga cttccttctc cacgacgacg ccgtccacct cccacgtgaa cgtcggaaac
     6241 ggctccatcc ggaagcccgt gagaaggcgc cagccctccg ggtggagggt gcccgggaag
     6301 cggttcgtcg agaggtcgac gcgcctcccg gcgaggacga gcgtctcctc gagcttcgag
     6361 aggagaaccg aacggcccgc gggcggccgc gtcgccgcca cgaggaggcc gtggtagcgc
     6421 cgcgtgttct cgccggtcgc ggtcgacatc gcgtagccgc cgaggccgtt cgtctcgagc
     6481 cactcgcccg aatcgcgtgt ctctgcggtc atgcggcttc ccccgtcagc cgaggaggat
     6541 cggcaggagg acgccgaggc ccaggacgaa gagccagagc gcgggagcgg gcgtgcccga
     6601 ggggcgcgtg cggcgctttt cacgggcggc gtggcgtgga tcgaggagga gccagcggga
     6661 ctgccgggcc tgctgggcca cgaagatgac cgccgtcagg gctccgaaga cgaggtgccc
     6721 gacgaggctc gggaggagag ctgaagccgc ggacgggcgc cagtcgatct cgccggtcgc
     6781 gaggagcggc agcagcgtca gaggtccgac gtaccaccag acgactccga acacgagtcc
     6841 ccacgagaga ccgcgaccga ggcccagccc ctcgcggcgg aagaggaagc cccagagcac
     6901 cccgaggagg gcgctcgcga aaaggtgcag gacgaggccg cgcgaaaggg agaggccgcc
     6961 ctcgacgccg gcgatcttcg ggaggacgcc ggtcgcgacg agaatcggcg cggcgacgat
     7021 cccgccgccg aggccggcga gagcgcccca gccggccgtg cggagcgtct gaacgccggg
     7081 gccctcgggc tcgcggcgga tcggatcggt ctcgacgaag agtctcacgc ggagcctctc
     7141 gacgaccgcg aagacgatgc cgacgatgac gccgtagaga acgtacccga cgagcggccc
     7201 gaagagatcg cccgcgtggt cggcggacca gtcgaggggc atgccggaga ggagcgggaa
     7261 gagggtcagc ggaaagacga accagcagag gatcccgtag cccacgcccc agcccatgct
     7321 cgagccgagc ccgtagagat cccgctggaa caggaggccg aagagcgagc cgatcgcgac
     7381 ggcgaagagg aagtggacgc tgaggccggc cggagagcgg cccgcttcac cgaggccggc
     7441 gaggagcggg aagaactcgc cgctcgccat ccagatctcg aagacggtcg ccgcgaggac
     7501 gcccgcgagg gagccgacgc cgacggcgcg gagcggcgag acgccggccg gcgtcccggg
     7561 ccgcccgacc ccgggcaggc cgaggacgag gccgaggggc gcg
//
LOCUS       DAOMFY010000188         7575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3056_length_7575_cov_8.271941, whole genome shotgun sequence.
ACCESSION   DAOMFY010000188 DAOMFY010000000
VERSION     DAOMFY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3056_length_7575_cov_8.271941"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLB01_13760"
     CDS             <1..375
                     /locus_tag="PLB01_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78415.1"
                     /translation="EPAPPAAGGVLSEAAPPAASELEPPEATAAAVLSYRDLAASRGV
                     RFLLVALDAPESRRMVAFARARGIDARILAAAPSLFLAEGHLNEEGNRQLAVYVARAL
                     TEPRAPSSSAGGRVPGVSAGGG"
     gene            complement(356..1681)
                     /locus_tag="PLB01_13765"
     CDS             complement(356..1681)
                     /locus_tag="PLB01_13765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQQ78416.1"
                     /translation="MRGCARAVLAGLSLAAHSALALAVTRGPYLQTATSTSVVVCWRT
                     DVPVDGRVFVGTSPGALSVAFSNASPATEHAIAVAGLAPRTRYYYAVGTASGVLAPAA
                     PASFVTPPPPGAAEPLRIWAVGDSGTADANARAVRDAYRAAAAGRPADLWLMLGDNAY
                     VAGTDAEYQAAVFGTYPAELASMPLWPAFGNHDGGSANGNTGKGVYFDVFRLPTAAEA
                     GGVASGSEAYYSFDAGNVHFVCLDAYDVSRFPGSPMLTWLAADLAATRQTWIVAYWHH
                     PPYTKGTHDSDTETELVEMRRYVNPILEAGGVDLVLCGHSHVYERSFLLDGHYGNSTT
                     LAPGMKKDGGTGDPAGTGPYRKPAARTPHAGTVYVVAGSGGQLGTGPLNHPAMLRSLS
                     ERGSLLIDVDAGRLDVRFVRADGAVRDSFAIVKSGGAVVGSPPFIPRRR"
     gene            complement(1681..3768)
                     /locus_tag="PLB01_13770"
     CDS             complement(1681..3768)
                     /locus_tag="PLB01_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQQ78417.1"
                     /translation="MEHRRLAGPLLAGILVWGGALALPSGRAAAQATSDYAVQVTAAV
                     QSAPPRITLTWPAFAGATAHTVYRKAWGVASWGTAVATLAGSATGYVDNGVVVGTRYE
                     YEVVRSASVTGTGYLATGIEVPLVEDRGKVVLVVDSSVAPALAAELARLQADLAGDGW
                     TVLRHDVARSATVPSVKALVKADYDADPTRVTSVFLFGHVPVPYSGSLAPDGHPDHVG
                     AWPADLYYGDMDGAWTDTQNLGGTGRQNNVAGDGKFDQSYAPGGTIELAVGRVDLANM
                     PAFAPKTEVDLLRQYLNKDHGFRVKAWTLPARGLIDDNFGAFGGEAFASTGWRAFSAF
                     FGPANVFALDWFSTLGANGYLWAYGCGGGNYTGAGGVGSTSNFVATDTKTAFTFLFGS
                     YFGDWDVSNDFLRAPLATTTYGLTCAWAGRPAWHVHHMAMGETIGYAARLTQNNSGTY
                     FYGYGPSVHVALMGDPTLRMHVVAPAANVTAAGGGGSATISWTASADAVAGYHVYRGA
                     GASGPFTRLTPSLVAGTSFVDAAAPGGNPAYLVRAVKLETSPSGSYWNASTGAANLTA
                     APVATTFRPLSPCRALDTRKPAGPGGGPSLGAGATRTFVAAGVCGVPATARSVSANVT
                     VTNTFAGGQIVLYAGDLPAPPGTVTVAFRAGQTRANNAVVGLASDASGSFRITSSAAA
                     PLDVVVDVNGWFE"
     gene            3852..5240
                     /gene="fumC"
                     /locus_tag="PLB01_13775"
     CDS             3852..5240
                     /gene="fumC"
                     /locus_tag="PLB01_13775"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQQ78418.1"
                     /translation="MTTRIEKDTMGEIAVPADRYWGAQTQRSLENFEIGEIRFTRPLI
                     RAFGLLKRAAAEVNRDLGLLPQEKAALVSAAAQEVADGKLDAHFPLVVFQTGSGTQTN
                     MNVNEVIANRAIDLAGGVVGSKNPIHPNDDVNMSQSSNDTFPAAMSIAAVCEIEERLL
                     PEVRDLRGVLDAKSKAFHDVVKIGRTHLMDAVPLTLGQEISGWVAQLDHGMAHVAQAL
                     PHLLELAIGGTAVGTGLNTHPEYAERVAHRVATLTGHPFVTAPNKFEALAAHDALAFA
                     SGALRTLAGSLMKIANDVRWLASGPRSGIGEIRIPENEPGSSIMPGKVNPTQAEALTM
                     VVAQVYGNDAAVAFAASQGNFELHVLKPVMIHNVLESVRLLADGCRSFRLHCAGGIEP
                     DRARIADLVARSLMLVTALNPHIGYDKAAAIAKKAHEEGTTLREAALALGTVTAEEFD
                     RWIDPSKMTGPG"
     gene            5204..6688
                     /locus_tag="PLB01_13780"
     CDS             5204..6688
                     /locus_tag="PLB01_13780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ78419.1"
                     /translation="MDRPVEDDRPRLTRAAGLALAVLAALAAGDQTGPSFTVAAPQAM
                     VTGRVRLEAFAQDARVESVRWEVDDWARTTARPFALEFDLGPVPREATVTAVALDAAR
                     QALYRRQAVLNPGGRRLVLEFLSPLDGGKASGPTPVLVRASAPPGDSLESVVLDAPGG
                     PVPLAGDDPFTATVDLPADTAPLTARVKTARGRTAERTILLNGRGLAAATDAHVVEQT
                     VAVTRGGEPLTGLGPADFAVKDDRGKCEVREVRFLRDAPLVIGLSIDTSMSLQYTEAL
                     RTAAAGSFLARTIRAGDVAFLQRFGVAVSEVVPWTGDRSLLEKSVLELGYDGVPGTLL
                     HTAVLRALYAFQGGQGARALVLVTDGNAFEDDVEERDAVAHARQSGVKIYALGLPYTE
                     EIHTPVSVTAPDGSVTVTDKVSLRTYPPNVEVLRRLTDATGGRTYAVKKEKDLPRIFA
                     AIERDLRSQYLVSYVSNAKRKGVFHPVEVRASRGTVTTAAGFFY"
     gene            6797..>7575
                     /locus_tag="PLB01_13785"
     CDS             6797..>7575
                     /locus_tag="PLB01_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ78420.1"
                     /translation="MSDSSGGRGAYPRYVLLVLVLVYVFNFLDRQIVSILAERIKADL
                     GVTDAQLGFLYGTVFAVFFAVFGIPLGRLADAWDRRRLIAWGAAFWSLMTALSGFAKS
                     FPQLALARMGVGVGEASAAPAAYSLLSDWFPKEKRATVLGLYSSGIYVGAGLGLGIGG
                     LVVDRWDMAFPGSTAPFGLRGWQAAFLAVGLPGLVLAAWVRSLREPPRAASHPPLTTT
                     PVRTFLRESASVLPPFTLFALAAAGAGAAGVLGNVAAFLGLA"
BASE COUNT         1045 a         2754 c         2699 g         1077 t
ORIGIN      
        1 gagcccgccc cgccggctgc gggcggcgtc ctttccgagg ccgcgcctcc ggccgcctca
       61 gagctcgagc cgccggaggc gaccgcagcc gccgttcttt cctaccggga cctcgccgcc
      121 tcacgaggcg tccggttcct cctcgtcgcc ctcgacgcgc ccgagagccg ccggatggtc
      181 gcgttcgcgc gcgcccgcgg aatcgacgcc cgcatcctcg ccgcagcgcc ctcgctcttc
      241 ctcgccgagg gccacctgaa cgaagaggga aaccgccagc tcgccgtgta cgtggcgcgc
      301 gccctcaccg agccgcgggc cccctcgtcg agcgcaggcg gccgcgtgcc tggcgtcagc
      361 gccggcgggg gatgaacggg ggggagccga cgacggctcc gccgctcttg acgatcgcga
      421 aggagtcccg cacggcgccg tccgcgcgca cgaagcgcac gtcgaggcgg ccggcgtcca
      481 cgtcgatcag gagcgagcct cgctcggaga ggctgcggag catcgccggg tggttcaagg
      541 gtccggtgcc gagctgtccg ccgctccccg cgacgacgta gaccgttccc gcatgcggcg
      601 tccgcgcggc cggtttgcgg tacggccccg ttcccgccgg gtctcccgtg ccgccgtcct
      661 tcttcatccc gggcgccaac gtcgtggagt ttccgtagtg cccgtcgagg aggaaggagc
      721 gctcgtagac gtgggagtgc ccgcagagga cgaggtccac gcccccggcc tcgaggatcg
      781 ggttcacgta gcggcgcatc tcgacgagct ccgtttccgt gtcggagtcg tgggtgccct
      841 tcgtgtacgg cgggtggtgc cagtacgcga cgatccaggt ctgtctcgtc gcggcgaggt
      901 cggccgcgag ccacgtgagc atcgggctcc cgggaaagcg gctcacgtcg tacgcgtcga
      961 gacacacgaa gtgcacgttc cccgcgtcga aggagtagta cgcctcgctg ccggacgcga
     1021 cgcccccggc ctccgccgcg gtcgggagcc ggaagacgtc gaagtagacg cccttgcccg
     1081 tgttgccgtt cgccgagccg ccgtcgtggt tgccgaacgc cggccagagc ggcatggacg
     1141 cgagctccgc gggatacgtc ccgaagaccg cggcctggta ctcggcgtcg gtgcccgcga
     1201 cgtaggcgtt gtcgccgagc atgagccaga ggtccgccgg ccggccggcc gcggcggcgc
     1261 ggtaggcgtc gcgaacggcg cgcgcgttcg cgtcggccgt gcccgagtcg ccgacggccc
     1321 agatccggag cggctcggcg gcaccgggcg gcggcggggt gacgaaggaa gccggggcgg
     1381 cgggcgccag cactcccgaa gcggtcccga cggcgtaata gtagcgcgtc cggggggcga
     1441 ggccggccac cgcgatcgcg tgttcggtgg cgggagaggc gttcgagaac gcgacggaga
     1501 gggccccggg gctcgtgccc acgaagacgc gcccgtccac gggtacgtcc gtgcgccagc
     1561 agacgacgac ggacgtgctc gtcgcggtct gaaggtacgg tccgcgcgtg acggccagcg
     1621 cgagcgcgga atgagccgcg agggagagcc cggcgaggac ggcgcgcgcg cagccccgca
     1681 ctattcgaac cagccgttga cgtccacgac gacgtccagc ggagccgctg ccgaactcgt
     1741 gatcctgaag gagccggaag cgtcggaggc gaggcccacg acggcgttgt tcgcgcgcgt
     1801 ctggccggcg cggaaggcga ccgtcaccgt tcccggcggc gccggaaggt cgccggcgta
     1861 gaggacgatc tgcccgcccg cgaacgtgtt cgtgacggtg acgttcgcgg agaccgatcg
     1921 ggcggtcgcg gggacgccgc acacgccggc cgccacgaag gtccgcgtgg ccccggcgcc
     1981 gagtgacggc ccgccgcccg ggccggcggg ctttcgggtg tccagggcgc ggcacggaga
     2041 gagcgggcgg aacgtcgtcg cgacgggcgc ggcggtcagg ttcgcggcgc ccgtgctcgc
     2101 gttccagtac gagccgctcg gactcgtctc gagtttcacc gcgcggacga ggtaggccgg
     2161 gttccccccg ggcgccgccg cgtcgacgaa agacgttccc gccacgagag agggcgtgag
     2221 gcgcgtgaac gggcccgacg cgccggctcc ccggtagacg tggtatccgg cgaccgcatc
     2281 ggcggatgcc gtccaggaga tcgtcgcgct tccgcctccg ccggcggccg tcacgttcgc
     2341 cgcgggggcc acgacgtgca tccggagggt cggatctccc atgagggcga cgtgcaccga
     2401 gggaccgtat ccgtagaaat atgttcccga gttgttctgc gtcaggcgcg ccgcgtaccc
     2461 gatggtctcg cccatcgcca tgtggtgcac gtgccacgcg ggccgtccgg cccacgcgca
     2521 cgtgaggccg taggtcgtcg tcgcgagcgg cgcgcgcagg aagtcgttcg aaacgtccca
     2581 gtccccgaag tagcttccga acaggaacgt gaacgcggtc ttcgtgtcgg tggcgacgaa
     2641 gttggacgtc gagcccacgc cgcccgcccc ggtgtagttc ccgccgccgc aaccgtaggc
     2701 ccagaggtat ccgttcgctc cgagcgtcga gaaccagtcg agggcgaaga cgttcgcagg
     2761 cccgaagaac gccgagaacg cgcgccagcc cgtcgaggcg aacgcctcgc ctccgaacgc
     2821 gccgaagttg tcgtcgatga ggccccgcgc ggggagcgtc caggccttga cccggaagcc
     2881 gtggtccttg ttcagatact ggcggaggag gtcgacttcg gttttcgggg cgaacgcggg
     2941 catgttcgcg agatcgaccc ggccgacggc gagctcgatc gtcccgccgg gggcgtagct
     3001 ctggtcgaac ttgccgtccc ccgcgacgtt gttctgccgg ccggtcccgc cgaggttctg
     3061 tgtgtccgtc caggcgccgt ccatgtcgcc gtagtagagg tccgcgggcc aggctccgac
     3121 gtggtccggg tgcccgtcgg gcgcgaggct gcccgagtac gggaccggaa cgtgcccgaa
     3181 gaggaagacg ctcgtcacgc gggtcggatc cgcatcgtag tcggccttga cgagggcctt
     3241 gacggacggg accgtcgcac tgcgcgcgac gtcgtgcctc aggaccgtcc agccgtcgcc
     3301 ggcgagatcc gcctggaggc gcgcgagctc ggcggcgaga gccggggcca cggaggagtc
     3361 gacgacgagg accacctttc cgcggtcctc gacgagcgga acttcgatcc ccgtcgcgag
     3421 gtatccggtg cccgtgaccg acgcgctccg gaccacctcg tactcgtatc tcgtcccgac
     3481 gaccactccg ttgtccacgt aaccggtcgc cgaaccggcg agcgtcgcca cggccgtccc
     3541 ccaggaggcg acgccccagg ccttccggta gaccgtgtgc gccgtcgcgc cggcgaacgc
     3601 gggccaggtg agcgtgatcc tcggcggtgc actctggacg gcggccgtca cctggaccgc
     3661 gtagtcagac gtcgcctgcg cggcggcgcg cccggaaggc agggcgaggg ccccgcccca
     3721 gacgagaatc cccgccagca agggccccgc gagcctgcga tgctccatct ctctgcagtg
     3781 aaatcgtctc acagcggtcg cggggccgcg cgtaacatgg cctgcgacgg agtctccggg
     3841 agggagcgtc catgaccaca cggatcgaaa aggacacgat gggcgagatc gccgtcccgg
     3901 cggaccggta ctggggcgcg cagacgcagc gctcgctcga gaacttcgag atcggcgaga
     3961 tccggttcac ccgcccgctg atccgcgcgt tcggcctcct caagcgcgcc gccgccgagg
     4021 tgaaccgcga cctcggcctc ctcccgcagg agaaggcggc gctcgtctcg gccgccgcac
     4081 aggaggtggc cgacggaaag ctcgacgcgc acttcccgct cgtggtcttc cagacgggca
     4141 gcgggacgca gacgaacatg aacgtgaacg aggtcatcgc gaaccgcgcg atcgacctcg
     4201 cgggcggcgt cgtcggctcg aagaacccga tccacccgaa cgacgacgtg aacatgtcgc
     4261 agtcgtcgaa cgacacgttt ccggcggcga tgtcgatcgc cgccgtctgc gagatcgaag
     4321 agcgcctcct tcccgaggtg cgggacctgc gcggcgtcct cgacgcgaag tcgaaggcgt
     4381 tccacgacgt cgtgaagatc ggccgtacgc acctcatgga cgcggtgccc ctcacgctcg
     4441 ggcaggagat ctccggctgg gtcgcgcagc tcgaccacgg gatggcccac gtcgcgcagg
     4501 cgctccccca cctcctcgag ctcgcgatcg gcgggacggc cgtgggaacg ggcctgaaca
     4561 cgcaccccga gtacgcggag cgcgtcgcgc accgtgtcgc gacgctcacg gggcacccgt
     4621 tcgtcacggc cccgaacaag ttcgaggctc tcgcggcgca cgacgcgctc gcgttcgcgt
     4681 ccggcgcgct ccggaccctc gcgggctccc tcatgaagat cgcgaacgac gtgcgctggc
     4741 tcgcctcggg cccgcgctcc gggatcggcg agatccggat cccggagaac gagccgggct
     4801 cgtcgatcat gccgggcaag gtcaacccga cgcaggccga ggcgctcacg atggtcgtcg
     4861 cgcaggtcta cggcaacgac gcggccgtcg cgttcgcggc cagccagggc aacttcgagc
     4921 tccacgtcct gaagcccgtg atgatccaca acgtcctcga gtccgtgcgc ctccttgcgg
     4981 acggctgccg gagcttccgc ctgcactgcg ccgggggaat cgagccggat cgcgcgcgga
     5041 tcgcggatct cgtcgcgcgg tctctgatgc tcgtcacggc gctcaacccg cacatcgggt
     5101 acgacaaggc ggccgcgatc gcgaagaagg cgcacgagga aggcacgacg ctccgggaag
     5161 ccgcgctcgc gctcggcacc gtgaccgcgg aagagttcga ccgatggatc gacccgtcga
     5221 agatgaccgg ccccggctga cgcgtgcggc ggggctcgct ctcgcggttc tggcggctct
     5281 cgccgccggc gaccagaccg gcccttcgtt caccgtcgcc gctccacaag ccatggtcac
     5341 gggccgcgtg cggctcgagg ccttcgcgca ggatgcgcgc gtcgagagcg tgcggtggga
     5401 ggtcgacgac tgggcgcgca cgacggcgcg cccgttcgcg ctcgagttcg acctcggccc
     5461 ggttccccgc gaagcgacgg tgaccgcggt cgcgctcgac gcggcgcgac aggctctcta
     5521 ccgacggcag gccgtcctga acccgggcgg ccggcgcctc gtcctcgagt tcctctcccc
     5581 gctcgacggc gggaaggcga gtggcccgac cccggtgctc gtgcgcgcgt cggcgccgcc
     5641 gggcgactcc ctcgagtccg tcgtgctcga cgcccccggc ggccccgtgc cgctcgcggg
     5701 cgacgacccc ttcaccgcga ccgtcgatct cccggccgac accgcgccgc tcacggcgcg
     5761 cgtgaagacg gcccgcgggc ggacggccga gcgcacgatc ctcctgaacg ggcgcggcct
     5821 cgccgcggcg accgacgcgc acgtcgtcga gcagacggtg gcggtcacgc gaggcggcga
     5881 gccgctcacg gggctcggac cggcggactt cgccgtgaag gacgaccgcg gcaaatgcga
     5941 ggtccgcgag gtgcgcttcc tccgcgacgc gccgctcgtg atcggcctct cgatcgacac
     6001 gtcgatgtcg ctccagtaca ccgaggcgct gcggacggct gcggccgggt ccttcctcgc
     6061 ccggaccatc cgcgccggcg acgtcgcgtt ccttcagcgc ttcggcgtgg ccgtctcgga
     6121 ggtcgttccg tggacgggcg accgctcgct cctcgagaag agcgtcctcg agctcggtta
     6181 cgacggcgtg cccggcacgc tcctccacac ggcggttctc cgcgcgctct acgcgttcca
     6241 gggcggccag ggggcgcgcg ccctcgtcct cgtgaccgac ggcaacgcgt tcgaggacga
     6301 cgtcgaggaa cgggacgcgg tcgcccacgc gcggcagtcg ggcgtcaaga tctacgcgct
     6361 cggcctgccg tacacggagg agatccacac gccggtctcc gtcacggcgc ccgacggcag
     6421 cgtcacggtg accgacaagg tctcgcttcg gacgtatccg ccgaacgtcg aggtcctccg
     6481 gcgcctcacg gacgcgaccg gcggccgcac gtacgccgtg aagaaggaga aggacctccc
     6541 ccgcatcttc gccgcgatcg agcgcgacct ccggtcgcag tacctcgtct cgtacgtgag
     6601 caacgcgaag aggaagggcg tcttccaccc cgtcgaggtc cgcgcctcgc gcggcaccgt
     6661 tacgacggcg gcgggattct tctactgaga gaccgggttt gaagattttc ttagagagtt
     6721 aagaggggcg cgcgccttcc gggccgccgt ttctcttcat tcgaagaaat cccccgtgtc
     6781 cctctaacat cctcgcatgt ccgacagctc cgggggccgc ggggcgtatc cacgctacgt
     6841 cctcctcgtc ctcgtcctcg tctacgtctt caatttcctc gaccggcaga tcgtctcgat
     6901 cctcgccgag cgcatcaagg ccgacctcgg cgtcacggac gcgcagctgg gattcctcta
     6961 cggaacggtc ttcgcggtct tcttcgccgt cttcgggatc ccgctcggcc gcctcgcgga
     7021 cgcctgggac cggcgccggc tcatcgcgtg gggcgcggcg ttctggagcc tcatgacggc
     7081 gctctcgggc ttcgcgaaga gcttccccca gctcgcgctc gcgcggatgg gcgtcggcgt
     7141 gggcgaggcc agcgcggcgc ctgccgcgta ctcgctcctc tcggactggt tcccgaagga
     7201 gaagcgcgcg acggtcctcg gcctctactc gagcggcatc tacgtcggcg cgggcctcgg
     7261 ccttgggatc ggcggcctcg tcgtcgaccg ttgggacatg gccttccccg ggagcacggc
     7321 gcccttcggc ctgcgcggct ggcaggccgc cttcctcgcc gtcggcctgc ccggcctcgt
     7381 tctcgccgct tgggtgcgca gcctccggga gcccccccgc gccgcgtcgc acccgccgct
     7441 cacgacgacg cccgtgcgga cgttcctccg cgaatcggct tcggtgctcc cgcccttcac
     7501 gctcttcgcg ctcgccgcgg cgggcgccgg cgcggcgggt gtcctcggaa acgtcgccgc
     7561 gttcctcggt ctcgc
//
LOCUS       DAOMFY010000189         7572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3062_length_7572_cov_6.364640, whole genome shotgun sequence.
ACCESSION   DAOMFY010000189 DAOMFY010000000
VERSION     DAOMFY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3062_length_7572_cov_6.364640"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PLB01_13790"
     CDS             complement(<1..1193)
                     /locus_tag="PLB01_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQQ78421.1"
                     /translation="MTAVEPPVPERVEGFGLYNFCVSPVHRPTSVQELAAVLASCRDR
                     GETPVFRGAGRSYGDAALNAAGPVVELTRLAAIRSLDAETGIVRAGAGLTLGELWKAS
                     IPKGLWPPVVTGTMNVTLGGAVAVNVHGKNGWKRGTIGDHLESVSVLRPDGRVEEIAA
                     GSAALADVVGNTRAADPIVEVALHLKKLRSGYLDVEGFATADLAATLSAIDGAKDDWE
                     YVVGWMDCWASGRALGRSALHVASEHVPAAGEPTGFSVEEQLADVRTGPLPVPLLLLG
                     LKLTARGPQMRLVNAAKFLAGRLAGRARYRQSLVAYSFLLDYLPGWNEAYRPGGFIQY
                     QLFVPKGKAEEAFSRALVLQQEAEVVSSLAVVKRHRADAFPRGYAPDGFSLALDFPVT
                     RGNSGR"
     gene            complement(1190..1933)
                     /locus_tag="PLB01_13795"
     CDS             complement(1190..1933)
                     /locus_tag="PLB01_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ78422.1"
                     /translation="MKRVVLVGATDGLGRALAAEYASRGCWVTILGRSPEKLAALVAE
                     LSARHPAATVRGVVCDLADASRIEPAFREALEASGHCDLFLYNAGLMPAGDGVTCDPE
                     SDAATMNVNAVAAVRMLGFAANYFRAARRGHLAAISSIAGDRGRKGNPAYNASKAALT
                     TFLEGLRNRLFPFGVTVSTVKPGFVGTRLLEGRKNLFWVVPPDVAARTIADRLEKRHE
                     VFYVYRRWGLFGLALRHVPRFLFKRMGPP"
     gene            complement(1965..2888)
                     /locus_tag="PLB01_13800"
     CDS             complement(1965..2888)
                     /locus_tag="PLB01_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HQQ78423.1"
                     /translation="MRSHGWYDLPPFQWDAQEHILNFIFLEGEEPVQVRVLRASTGLV
                     ATATALSSKGELRQRAPCSAPAAGRPSPPFKENLPFSSDVPTRSGVLGVLRRVLDLRA
                     ELSSFHALCAGDPRFAWIARRGAGRVLRAPTLFEDAVKVLATTNCTWGLTKVMVGNLI
                     ARYGNGGAFPPPAFVAGLSARELEKLKLGYRAPYLAAFADRVASGALDLARWEDLARP
                     DADVEKEIRAEKGFGPYAADTLGRLLGRHGKLGLDSWSRKKVSALRFRGRRVSDARVA
                     KVYAPFGAHAGLAFWLDVTRDWHEEKERLWP"
     gene            complement(2924..5365)
                     /locus_tag="PLB01_13805"
     CDS             complement(2924..5365)
                     /locus_tag="PLB01_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HQQ78424.1"
                     /translation="MNEPEPRPAALSAVAARVRALGAAASARLKVLAAWCGRQLGRVP
                     RPAWFALGATAVLLAVAAVVVVLYFSRIVSKTMDGRRWSLPTRLYSDVWVVRPGDALG
                     AEDVTRRLGRLRYAEVPKPPLLPGQYALSPKRVTVSVNDRETAWGRTPGVAAVLEFSG
                     RRVASVRRAEDGSPLPHVVFEPEVVGTVFDEKMQDRTLVTLDQVPKVLLDAILTTEDR
                     DFFTHAGISPRRLVGAVLQSVTRRSGVRGTSTLTQQLVKNMFLTPERTLKRKAVEALM
                     AVILESKYSKNQILEAYLNEIYLGQRGSVSVTGVEEASRFYFGKGVSRLELNEAAMLA
                     GLISSPGKYSPFRNPDNARQRRAVVLRGMFEQKKIDRAAFDAAIAAPLTPMAAPPTGV
                     VAPHFVDFVLKQAKEIPGFSKEDGTSVFTTLDPDMQQAAQAAVLKGLEELEKKFKRLR
                     ARGDEDQLQAALIALDPSTGSVRALVGGRDYQVSQFNRVVQARRQPGSLFKPFVYLTA
                     FGKRDLVPPITPASLYVDSPIALVWGTGEDETWSPKNYDGEYRGTMTARQALEQSINI
                     PTVRIAVTETAPGRTLLPDIVETARRAGITSPLRPYPSLALGSFETSPMEIASAFCTF
                     ANGGFRVKPNALLGLVTPSLRRMESKDEPIVRGADADAVSVLDSVLRGVVDRGTGGSA
                     RRLGAQGIFAGKTGTTNDGRDAWFVGFSPSLLVAVWVGFDDNRGLNLSGSHAALPIWA
                     DFVRRLPAHWFETPFPKTPGVVTASIDPTTGLLVTEECPASLDEVFLEGTEPQKRCPH
                     GAGGAPGPGIGGAGN"
     gene            5417..5959
                     /locus_tag="PLB01_13810"
     CDS             5417..5959
                     /locus_tag="PLB01_13810"
                     /inference="COORDINATES: protein motif:HMM:NF017778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HQQ78425.1"
                     /translation="MAKTLAELLRRLDAFPGRIPLGELEAALADVSMSLDDVRPWLIF
                     GADTYRRNLVHAGPAYQALVLCWRNGQRSPIHDHSGSSCAVRVLAGEALETVFERTRA
                     GMVFAVRSLAHAPGSVCATQDSDIHQVSNVADDGGDLVTLHVYSPPLLNMGTYTLFDV
                     NVRAFHEPVHEDALVDGGGI"
     gene            complement(5976..6194)
                     /locus_tag="PLB01_13815"
     CDS             complement(5976..6194)
                     /locus_tag="PLB01_13815"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HQQ78426.1"
                     /translation="MTRRLRFMVVYEEGLWYVHEVGSGQPMGAFDGQQNAVRTARHLV
                     EITGAELLVMNADGTLASREDGATEKTA"
     gene            complement(6264..7205)
                     /locus_tag="PLB01_13820"
     CDS             complement(6264..7205)
                     /locus_tag="PLB01_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HQQ78427.1"
                     /translation="MTAGRVLVPLLAAACLAPAVSSGGALVLGILAALTAGNPWPVET
                     KRLAHLFLGLSVMGLGAAADLGVVARVGARGIGYTAAGIALTCAAGFALARALRIEED
                     TAILVTAGTAICGGSAIAAVAPAIRAKAESVSVALATVFLLNAVALVVFPPLGRLLGL
                     DEARFGLFAALAIHDTSSVVGAAAAFGGKAVEVATTVKLARALWIVPLALLAARYHAR
                     RTGERAHARLAVPWFLGGFVAAAALVTWMPALAPAGHAVAGAARRLLVVTLFLIGTSL
                     TREAIGRVGARPVLLGLILWILAASATAAAILAGWIS"
     gene            7305..7466
                     /locus_tag="PLB01_13825"
                     /pseudo
     CDS             7305..7466
                     /locus_tag="PLB01_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
BASE COUNT         1127 a         2718 c         2739 g          988 t
ORIGIN      
        1 cgcccggagt tgccgcgcgt gaccgggaag tcgagcgcga gcgagaaccc gtccggcgcg
       61 tagccgcgcg ggaaggcatc ggcgcggtgg cgcttcacga cggccagcga cgagacgacc
      121 tccgcctcct gctgcaggac gagggcgcgc gagaacgcct cctcggcctt ccccttcggg
      181 acgaacagct ggtactggat gaagccgccc ggccggtacg cctcgttcca gccggggaga
      241 tagtccagaa ggaacgagta cgcgacgagc gactggcggt agcgcgcccg gcccgcgagc
      301 cggccggcga ggaacttcgc cgcgttcacg aggcgcatct gcgggccgcg cgccgtgagc
      361 ttcagcccca ggaggaggag cggcacggga agcggccccg tcctgacgtc ggcgagctgc
      421 tcttcgacgg agaacccggt cggctcgccg gccgccggaa cgtgctcgct ggcgacgtgc
      481 agagcactcc gcccgagcgc gcgccccgaa gcccagcagt ccatccagcc gacgacgtac
      541 tcccagtcgt ccttcgcgcc gtcgatcgcc gagagcgtcg ccgcgagatc cgccgtcgcg
      601 aagccctcga cgtcgaggta gcccgaacgc agcttcttga ggtggagtgc gacctcgacg
      661 atcgggtccg ccgcgcgcgt gtttccgacg acgtcggcca gcgcggccga gccggcggcg
      721 atctcctcga cgcgcccgtc cggccgcagg acggagacgc tctcgaggtg gtcgccgatc
      781 gtcccccgtt tccagccgtt cttgccgtgg acgttcacgg cgacggcgcc gccgagcgtc
      841 acgttcatgg tcccggtgac gaccggcggc caaaggccct tcgggatcga ggccttccag
      901 agctcgccga gcgtgagccc cgcgccggcg cggacgattc ccgtttcggc gtcgaggctg
      961 cggatcgcgg ccaggcgcgt gagctcgacg acgggcccgg cggcgttcaa cgccgcgtcg
     1021 ccatacgagc ggcccgcccc gcggaagacc ggcgtctcgc cgcggtcgcg gcagctcgcg
     1081 aggaccgccg ccagctcctg caccgacgtc ggccggtgga ccggcgacac gcagaagttg
     1141 tacaggccga agccctcgac gcgctcggga acgggaggtt cgacggccgt cacgggggcc
     1201 ccatgcgctt gaagaggaaa cgcggaacgt ggcggagcgc gaggccgaac aggccccagc
     1261 ggcggtagac gtagaagacc tcgtgccgct tttcgagacg gtccgcgatc gtccgggccg
     1321 ccacgtcggg cgggacgacc cagaagaggt tcttcctccc ctcgagcagc cgcgtgccga
     1381 cgaagccggg cttcacggtc gacaccgtca cgccgaacgg gaagagccgg ttgcgcagcc
     1441 cctcgagaaa cgtcgtgagc gccgccttcg acgcgttgta cgcggggttc cccttgcggc
     1501 cgcggtcgcc cgcgatcgac gagatcgccg cgaggtggcc gcggcgtgcc gcccggaagt
     1561 aattcgccgc gaaaccaagc atgcgaaccg ccgcgacggc gttgacgttc atcgtcgcgg
     1621 cgtcgctctc cgggtcgcac gtcacgccgt cgccggcggg catcagcccc gcgttgtaga
     1681 ggaagaggtc gcagtgtccg gacgcctcga gcgcctcgcg gaacgcgggc tcgatgcgcg
     1741 acgcgtccgc gaggtcgcag acgacgcccc ggaccgtggc ggcgggatgg cgcgcggaca
     1801 gctcggcgac gagcgcggcg agcttctccg gcgaacggcc gaggatggtg acccagcagc
     1861 cccgcgacgc gtactccgcc gcgagcgcgc gccccagccc gtccgtggcc ccgacgagga
     1921 cgactctctt cacgcgttct ccccgtccat cttacggcgg cccgtcacgg ccagaggcgc
     1981 tccttctcct cgtgccagtc gcgcgtcacg tcgagccaga acgcgaggcc cgcgtgggcg
     2041 ccgaacggcg cgtagacctt cgcgacgcgc gcgtcggaga ccctgcgtcc gcggaaccgc
     2101 agcgcggaca ccttcttccg cgaccacgag tcgagtccca gcttgccgtg ccgtccgagg
     2161 aggcgtccga gcgtgtcggc cgcgtagggg ccgaagccct tctcggcgcg gatctccttc
     2221 tcgacgtccg cgtcgggccg cgcgagatcc tcccagcgcg cgagatcgag cgcgccggat
     2281 gcgacgcggt ccgcgaacgc cgcgaggtac ggcgcccggt acccgagctt gagcttctcg
     2341 agctcgcggg ccgagagccc ggccacgaag gcgggcggcg ggaacgcgcc gccgttcccg
     2401 tagcgggcga tgaggttccc gaccatcacc ttcgtgaggc cccacgtgca gttcgtcgtc
     2461 gcgaggacct tcacggcgtc ctcgaacagc gtcggcgcgc gcaggacgcg ccccgccccg
     2521 cgccgcgcga tccacgcgaa gcggggatcg ccggcgcaaa gggcgtggaa agaggagagt
     2581 tctgctcgga ggtccaggac gcgccggagc acaccaagga cgcccgaccg agtcggaacg
     2641 tccgaggaga acgggagatt ttctttgaat ggtggagagg gccttcccgc cgccggagcg
     2701 ctgcagggtg cgcgctggcg caattcaccc ttcgaagaaa gcgccgtcgc agtcgcgacg
     2761 agtccagtcg acgcgcgcag gaccctcacc tgaacgggct cttcaccttc taagaagatg
     2821 aaattcagaa tgtgctcctg cgcgtcccac tggaacgggg gcaggtcgta ccacccgtgg
     2881 ctccgcacga cgaggtcgag gtcgaggggc gggagggtca gcctcagttg cccgctcccc
     2941 cgatgccggg gccgggcgcg ccgccggcgc cgtggggaca gcgcttctgc ggctcggtcc
     3001 cctcgaggaa gacctcgtcc agcgaggcgg gacactcctc cgtcacgaga agccccgtcg
     3061 tcgggtcgat cgacgccgtc acgacgccgg gcgtcttcgg gaacggcgtc tcgaaccagt
     3121 gcgccgggag gcggcgcacg aagtcggccc agatcggcag cgccgcatgc gagcccgaga
     3181 ggttcaggcc gcggttgtcg tcgaaaccca cccatacggc gacgaggagg ctcggcgaga
     3241 agccgacgaa ccatgcgtcg cgcccgtcgt tcgtcgtgcc cgtcttgccg gcgaagatac
     3301 cctgcgcgcc gagccgccgg gccgagccgc ccgttccgcg gtccacgacg cccctcagga
     3361 ccgagtcgag caccgacacc gcgtcggcgt ccgctccgcg aacgatcggc tcgtccttcg
     3421 actccatccg gcgcagggac ggcgtcacga ggccgaggag cgcgttcggc ttcacgcgga
     3481 acccgccgtt cgcgaacgtg cagaacgcgg aggcgatctc catcgggctc gtctcgaacg
     3541 agccgagcgc gagcgacggg tacggtctca gcggcgacgt gatcccggcg cggcgagccg
     3601 tctcgacgat gtcgggcagg agagtccgcc ccggcgccgt ctcggtgacc gcgatccgca
     3661 ccgtcgggat gttgatcgac tgctcgaggg cctgccgggc cgtcatcgtc cctcggtact
     3721 ccccgtcgta gttcttgggg gaccaggttt cgtcctcgcc cgttccccac acgagcgcga
     3781 tcggcgagtc gacgtagagc gacgcgggcg tgatcggagg gacgaggtcg cgcttgccga
     3841 acgcggtcag gtagacgaac ggcttgaaga gagagcccgg ctgccgccgc gcctggacga
     3901 cgcggttgaa ctggctcacc tggtagtcgc gcccgccgac gagggcccgc acggagcccg
     3961 tggaggggtc gagcgcgatc agcgcggcct ggagctggtc ctcgtcgccg cgggcgcgca
     4021 ggcgcttgaa cttcttctcg agctcctcca gccccttgag gaccgcggcc tgcgcggcct
     4081 gctgcatgtc ggggtcgagc gtcgtgaaga ccgacgtgcc gtcctccttc gagaagcccg
     4141 ggatctcctt cgcctgcttc aggacgaagt ccacgaagtg gggcgcgacg actcccgtcg
     4201 ggggcgccgc catcggcgtg agcggcgccg cgatcgcggc gtcgaacgcg gccctgtcga
     4261 tcttcttctg ctcgaacatg ccgcgcagga cgacggcgcg ccgctggcgc gcgttgtcgg
     4321 ggtttcggaa cggcgagtac ttgcccggcg acgagatgag gccggcgagc atcgcggcct
     4381 cgttgagctc gagccgcgag acgccctttc cgaagtagaa gcgcgacgcc tcttcgacgc
     4441 cggtcaccga cacggagccg cgctgcccga ggtagatctc gttgaggtag gcctcgagga
     4501 tctggttctt cgagtacttc gactcgagga tgaccgccat gagcgcctcg acggccttcc
     4561 gcttcagcgt gcgctcgggc gtcaggaaca tgttcttgac gagctgctgg gtcagcgtgg
     4621 acgtgccgcg gacgccggag cggcgggtca cgctctgcag gacggctccg acgaggcggc
     4681 gcggagagat ccccgcgtgg gtgaagaagt cccggtcctc cgtcgtgagg atcgcgtcga
     4741 ggaggacctt cgggacctgg tcgagcgtga cgagcgtgcg gtcctgcatc ttctcgtcga
     4801 agacggtgcc gacgacttcg ggctcgaaaa cgacgtgcgg aaggggcgaa ccgtcctcgg
     4861 cgcgccgcac cgatgcgacg cgtcgtcccg agaactcgag caccgccgcc acgcccgggg
     4921 tccggcccca cgccgtctcg cggtcgttca cggagaccgt gacccgtttc ggggagagcg
     4981 cgtactggcc cggcagcagc ggaggtttcg ggacctcggc gtagcgcagc cggccgaggc
     5041 ggcgcgtcac gtcctccgcc ccgagggcgt cgcccggccg cacgacccag acgtccgagt
     5101 acagccgcgt cggcaggctc cagcgccgtc cgtccatcgt cttcgagacg atgcgggaga
     5161 agtacaggac gacgacgacg gccgccacgg cgaggagaac cgccgtcgcg cccagcgcga
     5221 accaggccgg cctgggaacg cggccgagct gcctcccgca ccaggcggcg aggaccttca
     5281 agcgcgcgga cgccgccgcg ccgagggctc tcacgcgggc ggccacggcc gagagggccg
     5341 cggggcgcgg ctcgggctcg ttcacctcgg cgagtctagc cgaccgcatc cggtgctacg
     5401 atgcgattga atctcgatgg cgaagactct tgcggaactg ctccgccgcc tcgacgcctt
     5461 cccgggccgg atcccgctcg gcgagctcga agccgcactc gccgacgtgt cgatgtcgct
     5521 ggacgacgtc cggccgtggt tgatcttcgg ggccgacacg taccggcgga acctcgtcca
     5581 cgcgggaccc gcctaccagg cgctcgtcct gtgctggcgg aacggccagc gcagcccgat
     5641 ccatgaccac tccggctcga gctgcgccgt gcgcgtcctc gcgggggagg cgctcgagac
     5701 ggtcttcgag cggacacggg ccggaatggt gttcgcggtc cgctccctcg cccacgcccc
     5761 gggaagcgtc tgcgcgacgc aggactcgga catccaccag gtctccaacg tcgcggacga
     5821 cggcggcgac ctcgtgacgc tgcacgtcta ctcgccgccg ctcctgaaca tgggaaccta
     5881 cacgttgttc gacgtgaacg tgcgcgcgtt ccacgagccg gtccacgagg acgcgctcgt
     5941 ggacggcggc gggatctgac ggcgcccgcg ccgcgtcagg ccgtcttctc ggtggcgccg
     6001 tcctcgcggg acgcgagcgt tccgtcggcg ttcatgacga ggagttcggc gccggtgatc
     6061 tcgacgaggt ggcgcgcggt gcgaaccgcg ttctgctggc catcgaaggc gcccatcggc
     6121 tgtccgcttc cgacttcgtg cacgtaccag aggccctctt cgtacacgac catgaacctc
     6181 agacggcgcg tcacgttccc agtgtcgcgg ccgcggcgcg cccgcgccag agcatgatca
     6241 tgtcccccgg gcgcctcccc ggttcacgag atccagccgg cgagaatcgc cgcagccgtg
     6301 gccgacgccg cgaggatcca caggatcaag ccgagcagga ccggcctcgc tccgacccgc
     6361 ccgatcgcct cgcgcgtcag cgaggtgccg atcaggaaca gcgtgaccac gagcaggcgc
     6421 ctcgcggcgc ccgcgaccgc atggcctgcc ggcgcgagag ccggcatcca cgtcacgagc
     6481 gcagcggcgg cgacgaagcc tccgaggaac cacggaacgg cgaggcgggc gtgcgcgcgc
     6541 tctcccgtcc ggcgcgcgtg gtaccgggcc gcgaggagag ccagcgggac gatccacagg
     6601 gcgcgcgcga gcttcaccgt ggtcgcgacc tcgacggcct tcccgccgaa ggcagccgcc
     6661 gccccgacga cggagctcgt gtcgtggatc gcgagagcgg cgaagagccc gaaccgggcc
     6721 tcgtcgagcc cgaggaggcg cccgagagga gggaagacga cgagcgcgac cgcgttcagg
     6781 aggaacacgg tcgcgagggc cacggacacg gattcggcct tcgcgcggat cgcgggggcg
     6841 accgccgcga tcgcgctccc gccgcagatc gccgtccccg ccgtgacgag gatcgcggtg
     6901 tcctcctcga tgcgcagcgc gcgggcgagg gcgaagcccg cggcgcacgt gagcgcgatt
     6961 cccgccgccg tgtacccgat gccgcgggcg cccacgcgcg ccacgacgcc gaggtccgcc
     7021 gcggctccga gtcccatcac cgagaggccg aggaagaggt gcgcgaggcg cttcgtctcc
     7081 acgggccagg ggttgcccgc ggtcagggcg gcgaggatcc cgaggacgag ggccccgccc
     7141 gaggacacgg ccggcgcgag gcaggcggcc gcgagaagcg ggaccaggac gcggccggcg
     7201 gtcacgcgcg aagtgtaggc tagcgcgcga tgccggcccc gcaccgcgac gcgtccgtct
     7261 ccgtgcacgt cgtccgccgc gacggcgacg cggagagccg ggaccgggat tcactcacgg
     7321 ccgaggagcc gctcgagatc cggctccgcg acggcgacgc cgcgcccgag cggttcgtcg
     7381 tcacgatgcg cacgccgggc gacgacgacg acctcgccgt cggcctcctt tacggagagg
     7441 gcgtgatctc ggggatggac gacgtcgcgg cggtcggccc gccgggtgag cccggcgtcg
     7501 ccccggagct cgcacggaac gtccgcgtcg tggcgctcgc tcccggcgac gcgcgcggcg
     7561 caccgcggcg cg
//
LOCUS       DAOMFY010000190         7543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3077_length_7543_cov_6.922810, whole genome shotgun sequence.
ACCESSION   DAOMFY010000190 DAOMFY010000000
VERSION     DAOMFY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3077_length_7543_cov_6.922810"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="PLB01_13830"
     CDS             <1..1545
                     /locus_tag="PLB01_13830"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQQ78428.1"
                     /translation="RIGDFGFVLGIFGCFALFGTTDFGAMFSMAAADPAKYAAGGLMT
                     AVCLALFIGACGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVARCNVFFR
                     VSHEWAMKTLGEHSSVWGLLTHDASFVVAVVGGATALFAATMGLAQTDIKKVLAYSTV
                     SQLGYMFLGCGALAFSAGIFHVFTHAWFKACLFLGAGSVIHALGGEQDMTKMGGLRKL
                     IPHTFVTMFVATLAIAGVPGLAGFFSKDAILAETWGAGYKVLWVLAVLTAGLTAFYMF
                     RLIRMTFYGTFRGTAEQLKHVHESPASMTGPLYVLAAGAVVVGWFGIPKFILGSENSF
                     EKLLEPVITPLAAGWQPHPHEMSHATEWGLMGSSVLVAFLGIFLAVSFYSGAYPFRMP
                     ERIAASLGGFYRLVRDKYRVDELYEAVFVNGLIKKGGRLMWEIDARVLDLIPNGSSGL
                     TLFASRISSWFDRTFVDGAVNGVGSTLETAWRGMRRVQSGRTQNYALTMAAGVFGFVC
                     LYLLLK"
     gene            1559..3121
                     /locus_tag="PLB01_13835"
     CDS             1559..3121
                     /locus_tag="PLB01_13835"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQQ78429.1"
                     /translation="MNGLLSLLIFLPVLGTLAVFLVPREKPGLIRWVATLFTGAEFLL
                     SIPLWTFAGFRKSGDFFFRETVDWIPSLGAKYALGVDGIAALLILLTTLLSFISVYSS
                     FTAVTKREKEYYAFLLLLETGMIGVFCALDFFLFYVFWEVMLVPMYFLIGVWGGPRKL
                     YAAIKFFLYTLAGSVVMLLAIIALYFFNTSGFLWWKGLGNAPSFDIVHFQSIASQIPP
                     DLQIWLFWAFFLGFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFS
                     LPICPGASRTSWVQLSVVVLSIIGIIYGSMVAMVQKDMKKLVAYSSVAHLGFVMLGLF
                     ALNNAGIQGGILQSINHGLSTGGLFLLVGIVYERTHTRMIADYGGLSGVMPVYAMLFM
                     VILLASIGLPLLNGFVGEFTILTGAFLADWRWAAWACLGVILGAAYMLWLYQRVFFGE
                     LTNEKNKGLEDVNVREILTLAPLIVACFWIGLYPKPFFEMTAKSAEKIVAAVNAPAAS
                     ESAPRLAAAGVK"
     gene            3124..4578
                     /locus_tag="PLB01_13840"
     CDS             3124..4578
                     /locus_tag="PLB01_13840"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HQQ78430.1"
                     /translation="MPFAPADMLLILPELFLTAAGLVLLLAATVGGRERERTIALAAI
                     GSLVTTGLLLAVSVATAKGLPQSAFGGMFVLDRFSLYFKVLMLLAAAVTILFSLRFVG
                     ASPYPSGEYYGLILFATVGMLFMASGTHLVSIVVALELMALSQYVLAGYFKRETKSLE
                     AATKYFILGAFSSGVLIYGISLVYGATGTLSLAGISKALGAAPATPLLVVGIILVACG
                     MFFKIASAPFHVWAPDVYEGAPTPISAFFAVGPKLAAYAIFARIFFAGFGPRAGDWTA
                     IVAASAALTMLVGNIGALQQTNVKRLLGYSSIGHAGYALLGLLAYGDGTGIGLWAILV
                     YLLAYAFMNLGAFGLVILLESKGYAAESVDDFNGLSKKNPWAAAAMLVFLLSLAGIPP
                     TAGFVGKYLLFSAAMKAGWFWLSLLAVLMSAVSLFYYFRIARAMYLVDGEGELHWREE
                     PAVTAAIAICAAGTLAMGILPGPFVAFAKSCLFP"
     gene            4595..4876
                     /locus_tag="PLB01_13845"
     CDS             4595..4876
                     /locus_tag="PLB01_13845"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQQ78431.1"
                     /translation="MSGAAGPGNDEGPGGDRGFARYFADVLTFGWVLPAAIAAGAGLG
                     WLLDKVFGLYPVLTAVLAFMGLVAGLRQVYRESGVLSRSSGKGPGNDSA"
     gene            4863..5285
                     /locus_tag="PLB01_13850"
     CDS             4863..5285
                     /locus_tag="PLB01_13850"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HQQ78432.1"
                     /translation="MTAPEGHEAPGRPGRGWFRTAAVLALLAASGLAATGRFRPALAL
                     TLGAAVAIVSALWLSDFLGRFAAPERSTPARLDWKLGFKAVLRYGLIGAALWASVRAF
                     PADVPWLIGGLSTVVAAAALDGILEQVRGPRNGTGAAR"
     gene            5302..6093
                     /gene="atpB"
                     /locus_tag="PLB01_13855"
     CDS             5302..6093
                     /gene="atpB"
                     /locus_tag="PLB01_13855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQQ78433.1"
                     /translation="MHHEHSIFFGFFNALLTRILGPVDGAPAWWKNGVDLGGLHVAGV
                     APRGEHGELLGWIPDHLAMAIVVFLFCALFFLWASRRYRRDAPGPVQNVIEVFVDFLR
                     GVIKENIAHGEHYLPIVGAFFFFIFFSNICGLFFFLQPPTANLNVTFALSITCFLVFN
                     AEGIKAHGLIGYLKHFLGPMLALAPLMFLIEMIGNFARALSLSMRLYGNIYGEHTAGS
                     IFTGLMPIVVPWPMMALGIFTALLQAYVFTLLTTVYLGGATAHEH"
     gene            6129..6452
                     /locus_tag="PLB01_13860"
     CDS             6129..6452
                     /locus_tag="PLB01_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQQ78434.1"
                     /translation="MFKKLMSAGALGALALALPNLALAEEAAKAVSSGGAGWGHFSAA
                     FAIGLAAAAGAIAQGRTAVAACEGIARNPGAVGQIRGQAFLGLVLIESLVIYALVIAF
                     VINGK"
     gene            6572..7237
                     /locus_tag="PLB01_13865"
     CDS             6572..7237
                     /locus_tag="PLB01_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ78435.1"
                     /translation="MAFLTTKKASSPGAWDFKNGELPFADALYGAALRLTRNRQDAED
                     LLQETYLKAFAHYEGFTEGTNLKAWLFRILKNAFINGYRHAKVGPKEVDLGADGDGAL
                     EAAGTRARASANPGPEDQLVAGTMDGPVAEALAALPEDFRLAVELVDLQDFSYREVAD
                     ILEIPLGTVMSRLYRGRRLLESALLEYGRRRNYLGDGEPSRTRQNAPLPKTVQLSEYR
                     KRR"
     gene            7274..>7543
                     /locus_tag="PLB01_13870"
     CDS             7274..>7543
                     /locus_tag="PLB01_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQQ78436.1"
                     /translation="MDCRTASLLIEAYHDDELELVDAARLLAHFEACPECRSRCDEAA
                     RLRDTVRTGRPVDRCPEDLSRRLAARCVALPPRAPRRSLRPVVVVL"
BASE COUNT          980 a         2704 c         2407 g         1452 t
ORIGIN      
        1 cgcatcgggg acttcggttt cgtcctcggg atcttcggct gcttcgcgct cttcggcacg
       61 acggacttcg gcgcgatgtt ctcgatggcc gccgcggatc ccgcgaagta cgcggccggc
      121 ggcctcatga cggccgtctg cctcgcgctc ttcatcggcg cgtgcggcaa gagcgcgcag
      181 atcccgctct acgtctggct gccggacgcg atggcgggcc cgacgcccgt ctccgccctc
      241 atccacgcgg ccacgatggt cacggcgggc gtctacatgg tcgcgcgctg caacgtcttc
      301 ttccgcgtct cgcacgagtg ggcgatgaag acgctcggcg agcactcctc ggtttggggg
      361 ctcctgacgc acgacgcgtc cttcgtcgtc gccgtcgtcg gcggcgcgac cgctctcttc
      421 gccgcgacga tgggcctcgc ccagacggac atcaagaagg tcctcgcgta ctcgacggtc
      481 tcgcagctcg gctacatgtt cctcggctgc ggcgcgctcg ccttctcggc gggcattttc
      541 cacgtcttca cgcacgcgtg gttcaaggcc tgcctcttcc tcggggcggg gtccgtgatc
      601 cacgctctcg gcggcgagca ggacatgacg aagatgggcg ggctgcgcaa gctgatcccg
      661 cacacgttcg tcacgatgtt cgtcgcgacg ctcgcgatcg cgggcgtccc gggcctcgcg
      721 ggcttcttct cgaaggacgc gatcctcgcc gagacctggg gcgcggggta caaggtcctg
      781 tgggtcctcg cggtcctcac ggcgggcctc acggccttct acatgttccg gctcatccgg
      841 atgacgttct acgggacgtt ccgcggcacg gccgagcagc tcaagcacgt gcacgagtcg
      901 cccgcgagca tgaccgggcc gctctacgtc ctcgcggcgg gcgcggtcgt ggtcggctgg
      961 ttcgggatcc cgaaattcat cctcggaagc gagaattcct tcgaaaagtt gctggaaccg
     1021 gtcatcactc cattggcggc cggctggcag ccgcatcccc acgagatgtc gcatgcgacg
     1081 gagtggggcc tgatgggctc ctcggttctc gtcgcctttc tggggatctt cctcgccgtc
     1141 tcgttctact ccggcgcgta cccgttccgg atgccggaac gaatcgcggc gtccctcggc
     1201 ggcttctacc ggctcgtgcg cgacaagtac cgtgtcgacg agctctacga ggccgtgttc
     1261 gtgaacggac tcatcaagaa gggcgggcgc ctcatgtggg agatcgacgc ccgcgtcctc
     1321 gacctcatcc cgaacggctc gagcggcctc acgctcttcg cctcgaggat ctcctcgtgg
     1381 ttcgaccgga cgttcgtgga cggggcggtg aacggcgtcg gatcgacgct cgagacggcg
     1441 tggcgcggga tgcgccgcgt gcagtcgggc cgcacccaga actacgcgct gaccatggcg
     1501 gccggcgttt tcggtttcgt ctgcctctac ctcctcctga agtgaagaag gacgccgcat
     1561 gaacggactt ctctccctgc tcatcttcct gcccgtgctg gggaccctcg cggtcttcct
     1621 ggtgccgcgc gagaagccgg gcctcatccg ctgggtcgcg acgctcttca cgggcgccga
     1681 gttcctcctc tcgattccgc tctggacgtt cgcgggcttc cgcaagtccg gcgacttctt
     1741 cttccgcgag acggtggact ggatcccctc gctcggcgcg aagtacgcgc tcggggtcga
     1801 cgggatcgcg gcgctcctga tcctcctgac gacgctcctc tcgttcatct ccgtgtactc
     1861 gtcgttcacg gcggtcacga agcgcgagaa ggagtactac gcgttcctcc tcctcctcga
     1921 aacggggatg atcggcgtct tctgcgcgct cgacttcttc ctcttctacg tgttctggga
     1981 ggtcatgctc gtcccgatgt acttcctgat cggagtctgg ggcggcccgc ggaagctcta
     2041 cgcggcgatc aagttcttcc tgtacacgct cgcgggctcg gtcgtcatgc tgctcgcgat
     2101 catcgccctc tacttcttca acacctcggg cttcctctgg tggaagggtc tcggcaacgc
     2161 gccgtcgttc gacatcgtcc acttccagtc gatcgcctcc cagattccgc ccgatctcca
     2221 gatctggctc ttctgggcgt tcttcctcgg gttcgcgatc aaggtcccga tgttcccgtt
     2281 ccacacgtgg ctgccggacg cgcacgtcga ggcgcccacg gcgggctcgg tcatcctcgc
     2341 cggcgtcctc ctcaagatgg gaacgtacgg cttcctgagg ttctcgctcc cgatctgccc
     2401 aggggcgtcc cggacctcct gggtgcagct ctccgtcgtc gtgctctcga tcatcggcat
     2461 catctacggc tccatggtcg cgatggtcca gaaggacatg aagaagctcg tcgcgtattc
     2521 ctcggtcgcc cacctcggct tcgtcatgct cgggctcttc gccctgaaca acgcgggcat
     2581 ccagggcggc atcctccagt ccatcaacca cggcctctcg acgggcgggc tcttcctcct
     2641 cgtcggaatc gtctacgagc gcacgcatac ccgcatgatc gcggactacg gcggcctttc
     2701 gggcgtcatg ccggtctacg cgatgctctt catggtcatc ctgctcgcct cgatcggcct
     2761 gcccctcctg aacgggttcg tcggcgagtt cacgatcctc acgggcgcgt tcctcgccga
     2821 ctggcgctgg gccgcgtggg cgtgcctcgg cgtcatcctc ggcgccgcgt acatgctctg
     2881 gctctaccag cgcgtgttct tcggcgagct cacgaacgag aagaacaagg gcctcgagga
     2941 cgtcaacgtc cgcgagatcc tgacgctcgc gccgctcatc gtggcgtgct tctggatcgg
     3001 cctctacccg aagccgttct tcgagatgac cgcgaagtcg gccgagaaga tcgtcgccgc
     3061 ggtgaatgcg cccgcagcct ccgagtccgc gccgcgcctc gcggccgcag gagtcaagta
     3121 gccgtgccgt tcgcccccgc cgacatgctc ctcatcctgc cggagctgtt cctcacggcg
     3181 gcggggctcg tgcttctgct cgccgcgacc gtgggcggcc gcgagaggga gcggacgatc
     3241 gccctcgccg cgatcggctc gctcgtcacg accgggctgc ttctcgcggt ctcggtggcc
     3301 accgcgaagg gcctgccgca gtcggccttc ggcgggatgt tcgtcctcga ccggttcagc
     3361 ctctacttca aggtcctgat gctcctcgcg gccgcggtga cgatcctctt ctccctgaga
     3421 ttcgtcggcg cctcgccgta cccgtctggc gagtactacg ggctcatcct cttcgcgacg
     3481 gtcggaatgc tcttcatggc gtccggcacg cacctcgtct cgatcgtcgt cgcgctcgag
     3541 ctcatggcgc tctcgcagta cgtcctcgcg ggctacttca agcgcgagac gaagtccctc
     3601 gaggcggcca cgaagtactt catcctcggc gcgttttcct cgggcgtgct catctacggg
     3661 atctccctgg tctacggtgc gacggggacg ctctctctcg cggggatctc gaaggccctc
     3721 ggggccgcgc ccgccacgcc gctcctcgtc gtcgggatca tcctcgtcgc gtgcggcatg
     3781 ttcttcaaga tcgcctcggc gccgttccac gtctgggcgc cggacgtgta cgagggcgct
     3841 ccgacgccca tctcggcgtt cttcgccgtc gggccgaagc tcgcggcgta cgcgatcttc
     3901 gcgcgcatct tcttcgccgg attcggcccg cgcgcgggcg actggaccgc gatcgtggcc
     3961 gcgtccgccg cactcacgat gctcgtcggg aacatcggcg cgctccagca gacgaacgtc
     4021 aagcgcctcc tcggctactc ctcgatcggc cacgccgggt acgcgctcct cggcctcctc
     4081 gcctacggcg acggtaccgg gatcggcctc tgggcgatcc tggtctacct cctcgcctac
     4141 gcgttcatga acctcggtgc cttcggcctc gtgatcctgc tcgagtcgaa gggctacgcc
     4201 gccgagagcg tcgacgactt caacggcctc tcgaagaaga acccctgggc ggccgccgcg
     4261 atgctcgtct tcctgctctc gctcgccggc atcccgccga cggccgggtt cgtcggcaag
     4321 tacctgctct tctcggccgc gatgaaggcc ggctggttct ggctctcgct tctcgccgtc
     4381 ctcatgtcgg ccgtgtcgct cttctattac ttccggatcg cgcgggccat gtacctcgtg
     4441 gacggggaag gcgagctgca ctggcgcgag gagccggcgg tgaccgcggc aatcgcgatc
     4501 tgcgcggccg ggacgctcgc gatgggcatt ctccccggcc ccttcgtggc gttcgcgaag
     4561 agctgcctct tcccgtgagg ggagccgcgg cggaatgagt ggcgccgccg gtcccgggaa
     4621 tgacgagggt ccgggcggcg accggggctt cgcgcgctac ttcgccgacg tcctgacgtt
     4681 cgggtgggtc ctgcctgcgg ccatcgccgc gggcgccggc ctcggctggc tcctcgacaa
     4741 ggtcttcggc ctgtatccgg tcctgacggc ggtcctggcc ttcatgggcc tcgtggccgg
     4801 cctccggcag gtctaccgcg agtcgggagt cctgtcccgg agcagcggaa aaggccccgg
     4861 aaatgacagc gcctgagggc cacgaggctc ccggcaggcc cggacgcggg tggttccgga
     4921 cggcggccgt cctcgccctc ctggccgcct ccggactggc tgccacgggc cgcttccggc
     4981 ccgcgctcgc cttgactctg ggggctgccg tggctatcgt ctcggcgctc tggctgtcgg
     5041 atttcctcgg tcgcttcgcg gcgcccgagc gaagcacgcc ggcccgactc gactggaaac
     5101 tcgggttcaa ggccgttctc cggtacggcc tgatcggagc cgctctgtgg gcctccgttc
     5161 gggctttccc ggccgacgtc ccgtggctga tcgggggtct ttcgacggtc gtcgccgcgg
     5221 cggcgctcga cgggattctc gaacaggtcc gcgggccgcg gaacggaacg ggcgcggccc
     5281 gctgaaggcg gaaacggcga cgtgcaccac gaacattcga tcttcttcgg gttcttcaac
     5341 gcgctcctga cgcggatcct cgggcccgtc gacggcgctc ccgcctggtg gaagaacggc
     5401 gtcgacctcg ggggcctcca cgtcgccggc gttgcgccgc gcggagagca cggagagctt
     5461 ctcgggtgga ttcccgacca cctcgcgatg gcgatcgtcg tcttcctctt ctgcgcgctc
     5521 ttcttcctct gggcctcgcg ccgctaccgc cgggacgcgc ccggccccgt gcagaacgtg
     5581 atcgaagtct tcgtcgactt cctgcgcggc gtcatcaagg agaacatcgc ccacggggag
     5641 cactacctcc cgatcgtggg tgcgttcttc ttcttcatct tcttctcgaa catctgcggc
     5701 ctgttcttct tcctgcagcc gcccacggcg aacctcaacg tgacgttcgc cctgtcgatc
     5761 acgtgcttcc tcgtcttcaa cgccgagggc atcaaggcgc acggcctgat cggctacctc
     5821 aagcacttcc tcgggccgat gctcgcgctc gcgcctctca tgttcctcat cgagatgatc
     5881 gggaacttcg cccgcgcgct ttcgctctcg atgcgtcttt acggcaacat ctacggcgag
     5941 cacacggccg gcagcatctt cacgggcctg atgcccatcg tcgtcccctg gccgatgatg
     6001 gcgctcggga tcttcacggc gctccttcag gcgtacgtgt tcacgctcct caccaccgtc
     6061 tatctgggcg gcgccaccgc ccacgagcac tgatcttgga tttctcatct cgaaaggagt
     6121 ctcacgcaat gttcaagaag ctcatgtccg cgggcgccct cggcgcgctc gcccttgccc
     6181 tcccgaacct cgccctcgcc gaggaagccg cgaaggccgt ctcgtccggc ggcgccggtt
     6241 ggggtcattt ctcggccgcg ttcgcgatcg gcctggccgc tgcggccggc gccatcgccc
     6301 agggccgaac ggcggtcgcg gcctgtgagg gaatcgcgcg caaccccggc gctgtcggtc
     6361 agatccgcgg ccaggccttc ctcggcctcg tgctgatcga atcgctggtc atctacgccc
     6421 tcgtcatcgc gttcgtcatc aacggcaagt aagtctcttc cggtaagtga cttggcggcc
     6481 ccgggcgacc ggggccgctg ctttttcggg gatgccggaa aagccgaatg aaaccggcgc
     6541 acgtattgct tggtggagta cggtgcgacc gatggcattc ctgactacga aaaaggcctc
     6601 ttccccggga gcctgggact tcaagaacgg cgagctgccg ttcgcggacg ctctctacgg
     6661 ggccgcgttg cgcctcacgc gcaaccgcca ggacgccgag gacctcctgc aggagaccta
     6721 cctgaaggcg ttcgcgcact acgagggctt cacggagggg acgaacctca aggcgtggct
     6781 cttccgcatc ctaaagaacg cgttcatcaa cggctaccgg cacgcgaagg tcgggccgaa
     6841 ggaagtcgac ctcggcgcag acggcgacgg cgccctcgag gccgccggga ctcgcgcgcg
     6901 ggcgtccgcg aacccgggcc ccgaggatca gctcgtcgcc gggacgatgg acggccccgt
     6961 ggcggaggcg ctggcggccc tgccggagga cttccgcctc gccgtcgagc tcgtcgacct
     7021 gcaggacttc tcgtaccgcg aggtggcgga catcctcgag atcccgcttg gaacggtcat
     7081 gtcgcgcctg taccggggcc ggcgcctcct cgagtcggcg ctcctcgaat acggccggcg
     7141 gcgcaattac ctcggcgacg gcgagccctc gcggacgcgt cagaacgccc ccctgccgaa
     7201 gaccgttcag ttgtcggaat accgcaagcg ccggtaacgt tgagcggttt ggcggggtcg
     7261 accgccgctg acgatggatt gccggacggc cagcctcctc atcgaggcgt accacgacga
     7321 cgagctcgag ctcgtcgacg ccgcccgtct cctcgcccac ttcgaggcct gcccggaatg
     7381 ccgatcccga tgcgacgagg ccgcgcgcct gcgcgacacg gtgcgcaccg gacggccggt
     7441 ggaccgctgc cccgaggacc tatcccggcg tctcgccgca cgctgcgtcg cgcttccgcc
     7501 gcgcgccccg cgccgctcgc tgcggcccgt cgtcgtcgtc ctc
//
LOCUS       DAOMFY010000191         7540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3079_length_7540_cov_8.358317, whole genome shotgun sequence.
ACCESSION   DAOMFY010000191 DAOMFY010000000
VERSION     DAOMFY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3079_length_7540_cov_8.358317"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..980
                     /locus_tag="PLB01_13875"
     CDS             27..980
                     /locus_tag="PLB01_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HQQ78437.1"
                     /translation="MVPCRTYRLVLAGSLLAVVSLFTFEGNLRAAAPAAAPKQKTFSS
                     PTAAADALISAAAKFDVPALTEIFGSDGIDLVVSGDAVQDRNTAGAFARKGLEKRSVA
                     PDPLNPGHMVLLLGDDNWPSPIPLVKANGGWRFDTKAGRREVLYRRIGGNELDAIKFC
                     RGYVEAQHEYASKKRDGSRVNQYAQRVIATPGKQDGLAWKNADGTWGGPVGENVARVI
                     TEGYSDKSKPYHGYYFKVLKGQGPAAPLGEMDFVVKGVMIGGFALVAAPAEYRVTGVK
                     TFMVSHTGVVYQKDLGEKTLEAFKAMERFNPDKTWQVVPGE"
     gene            986..2338
                     /locus_tag="PLB01_13880"
     CDS             986..2338
                     /locus_tag="PLB01_13880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ78438.1"
                     /translation="MGGRGWGSVALVALLALALRAAIFFAVGFNPAFFDADGYHAQAL
                     EGLAGRPVPTDGHPPGYPWFLMAIYRIAGPRPRIVYAIQVLLSAAAVFLAGDAARRRF
                     GPRAGVLTAALLATSGILAFYPSLLVSENLCLLGVAGIAWLLLPDHPEVSPARLLCCA
                     VIVAGLALTRTGLLLLALPLVAIPFFDAIRRRQARGLALAALTLAVATAPALAYSWHR
                     AGRTGTFRLGSPADTQSLYFGNNPNATGRTEWFPEAQPGTPEAPDYESLARILGPKAR
                     TYFLTHPLREADLFLRRASFNFAPNKRDIIYLYGHGWAGERSPGTLNALYVWIAVGVP
                     VLGAAVLLALARSPREGALRLALLLVFLGVLPYQVSVGDARYLIPFHPLLAFAAGALA
                     ASPPSPGQWPARMRLAAAVLAIAFLGNAAYDVWKTDGALRKVTQPGGSRLRPPYIFAR
                     "
     gene            complement(2347..4857)
                     /locus_tag="PLB01_13885"
     CDS             complement(2347..4857)
                     /locus_tag="PLB01_13885"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQQ78439.1"
                     /translation="MLPVPRFALVVALLGGAPAYAQDLKVVAELGRVGPPTALSVQTL
                     TKGGSPSLVSVGRGFVNVWLLDGSQEPVVTAIPETYRQFTFGDFDGDGFLDLAAAGGA
                     GIDILKGDGKGSFAPSLSLARSGSFNDLSAADVDGDGRPDLVVVDGVTIQSFLSRGGL
                     RFDGPFVTPITTYGGAGTVSAGDLDGDGRADVVLSGMYRGYPSVWLGAGDGTFRVTSV
                     SLPGRSPGRVLLADLTGTGRLDLAYPNSVYIGPDTLIIYANDGHGQFRGAGGCLAVPG
                     LVTPSALDVDGDGRNELVDLFRADHYSLVVCRADGPPEGERLVLPLFSPLLEIYSVAA
                     LRYPAAGATFFLSTREGIVVLARAGARTDRSIVPVVVSTTGLFGSRFDSDLLLTNSGS
                     TPAHVILRYAAADGGGSGVVEKDLGAGAQLIAPSAVKFLRDEGLPIGVGNVVGTLRLD
                     VTGASSPRAVTASVRTTSPSGAGVSCGGVPLASALRGPAIVPWLKEDARDRTNLALVN
                     AGGDAEGPITLRVTVHPGTAGAESPAVLPDVTLPPGGFFQFGRILRTAGLAASVGWAR
                     VERVSGGAPYLSWAVVNDAGTSDGSFVAGVVEEDRAETREIPNAVQTARYTTELVATN
                     PGDSPLGLVVRLGADPFQGPSFTLLISAGETSYVPDLFAEFRRRGVPGAPPAEVEIVS
                     SVSLSASGFIAAGVRVSSSPAPGRSYGVFESARYVGRADSVVIPDLRQDAGTRSNVGV
                     SPGSGFGFIDFRVEIRDPSGALAGVRDGVHVESGVLQMNAILRNVAPGLARGWARIIP
                     SLPVEYAPPAFTAYGVINDGAEPGQGTDDGSFVPAVPE"
     gene            complement(4951..5964)
                     /locus_tag="PLB01_13890"
     CDS             complement(4951..5964)
                     /locus_tag="PLB01_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78440.1"
                     /translation="MKRIAASAAVLALASTLALPAAAATDSALALVPADAASVGVIHV
                     ADLRTSPLAARVFSDTDRIAVDGDAAHFLAEARLNPKEDVDTVVAAGSPKGAGGSAGW
                     GLVLFEGRFDVAALSSAIAARGAERKSTPAGDYFLLPDKGQNAARHEPGAIAFASNHL
                     VIAGNEASVVAALAQRSAGGTAFRSGAGLGRHLGRIDSGASAWALVDVTRIPAKDAAM
                     RHAHVQAEGKGMDAAATLVASMKNVSLLALQATAKGDALKLNATGVASDDETAGLMED
                     ALRGVLAAWRLAAQEKSPDTVAVLRKFAVTRDRSTVSVSGTLPGATVRALAEKKHAKA
                     QVD"
     gene            complement(5967..6821)
                     /locus_tag="PLB01_13895"
     CDS             complement(5967..6821)
                     /locus_tag="PLB01_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ78441.1"
                     /translation="MRALAANIEEVFREAAARWTLVAYFALSSLFILLFAVAVNLDVV
                     NGALAGAKIFGANVHMGRQSVDLDKLVLGFESGFAGFLYVVGTFLAIFATAHLVPRLM
                     EKGTVDLYLSRPVGRVPLLLSRYLGGVLLSATNVLYLLGSMWLLVIWKTRLVHPRFFL
                     AAGIILFGIAALMAFAFLVGTLTSSTAVSIMATFALFFLSAILVNHDRIAAAMSYEWA
                     AQLVQGLYWVFPKTAELGTAVVALVMGDQGPARIQKALALQPFLTTGAFGAACLALAC
                     LRFRRKDF"
     gene            complement(6834..>7540)
                     /locus_tag="PLB01_13900"
     CDS             complement(6834..>7540)
                     /locus_tag="PLB01_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78442.1"
                     /translation="RQTLSVFGRMSGVDHATLKARIPGLLERVKLGDWMDVKVKKFSK
                     GMTQRLGLAAALVHEPEVLLLDEPTDGVDPVGRREIRDLLRAEASRGRAILLNSHLLS
                     EIELTCDRVAVLRKGKVAAMGTVEELTKKDEEKTSLKGYKLVASPLSEELLSDFFERG
                     ANPERVNGHVRVLARDAQHLNSLIDAARQRGALLTELTPEKSTLEDVFVDLVKATPDA
                     EPPSSPSKKSSNGLTN"
BASE COUNT         1204 a         2625 c         2624 g         1087 t
ORIGIN      
        1 acacgaagaa cgggggattt cttcgaatgg tcccgtgccg aacgtaccgc ctcgtcctcg
       61 ccggctcgct cctcgccgtc gtctcgctct tcaccttcga aggaaatctc agggcggccg
      121 cgcccgcggc cgctccgaag caaaagactt tctcttcgcc gacggcggca gccgacgcgc
      181 tcatctccgc ggccgcgaag ttcgacgtgc ccgccctcac cgaaatcttc gggtcggacg
      241 gcatcgacct cgtcgtctcg ggagacgccg tccaggacag gaacacggcc ggcgcgttcg
      301 cccgcaaggg tctcgagaag cgttccgtcg cgcccgaccc gctgaacccg ggccacatgg
      361 tcctcctgct gggcgacgac aactggccct ccccgatccc gctcgtcaag gcgaacggag
      421 gctggcggtt cgacacgaaa gccgggcgcc gggaggtcct ctaccgccgc atcggcggca
      481 acgagctgga cgcgatcaag ttctgccggg gatacgtcga ggcccagcac gagtacgcgt
      541 cgaagaagcg cgacgggtcg cgcgtgaacc agtacgccca gcgcgtcatc gcgacaccgg
      601 gcaagcagga cggccttgcg tggaagaacg cggacgggac gtggggcggc ccggtcgggg
      661 agaacgtcgc gcgcgtcatc accgagggct actccgacaa gtcgaagccg taccacggct
      721 actacttcaa ggtcctgaag gggcagggcc ccgcggcccc gctgggcgaa atggacttcg
      781 tcgtcaaggg cgtgatgatc ggcgggttcg cgctcgtcgc ggccccggcg gaataccgcg
      841 tcaccggcgt gaagacgttc atggtcagcc acaccggcgt cgtctaccag aaggacctcg
      901 gcgagaagac gctcgaggcc ttcaaggcca tggagcgctt caacccggac aagacgtggc
      961 aggtggtccc gggcgagtag tcgacatggg ggggagaggc tgggggtcgg tcgcgctcgt
     1021 cgcgctgctg gccctcgccc tccgggccgc gatcttcttc gcagtcgggt tcaacccggc
     1081 gttcttcgac gccgacggct atcacgccca ggcgctggaa gggttggcgg gccggcccgt
     1141 tcctaccgac ggacacccgc cgggctatcc ctggttcctc atggcgatct accggatcgc
     1201 gggaccccgg ccgcggatcg tgtacgcgat ccaggtcctt ctctcggcgg ccgccgtgtt
     1261 tctcgcgggc gacgcggccc ggcgtcgctt cggcccgcgc gccggcgtgc tgacggcggc
     1321 gctcctcgcc acgagcggca tcctcgcttt ctacccgtcg cttctcgtga gcgagaacct
     1381 ctgcctcctc ggcgtggccg ggatcgcgtg gctgctcctg ccggaccatc ccgaggtttc
     1441 cccggcgcgg ctcctgtgct gcgccgtgat cgtggccggg ctcgcgctca cgcgcaccgg
     1501 tctgctcctc ctcgcgctgc ctctcgtcgc gatccccttt ttcgacgcga tccgccggcg
     1561 gcaggctcgc gggcttgcgc tcgccgcgct cacgctcgct gtcgcgacgg cgcccgcgct
     1621 ggcctactcg tggcatcgcg cgggccgcac cggcaccttc cggctcggct cgcccgccga
     1681 cacgcagagc ctctacttcg gcaacaaccc gaacgccacg ggacgcaccg agtggttccc
     1741 ggaggcgcag cccgggactc ccgaagcgcc cgactacgaa tcgctcgcgc ggatcctcgg
     1801 accgaaggcg aggacgtatt tcctcacgca tccgctccgg gaagcggacc tgttcctgag
     1861 gcgcgcgtcg ttcaacttcg cgccgaacaa gcgggacatc atctacctct acgggcacgg
     1921 gtgggccgga gagcgttcgc ccggaacgct gaacgccctc tacgtctgga tcgcggtcgg
     1981 agtgcccgtc ctgggagccg cggtgctcct cgcgctcgcg cgaagcccgc gagagggcgc
     2041 gctccggctg gcgctcctcc tcgtcttcct cggcgtcctt ccctaccagg tctccgtcgg
     2101 cgacgcccgc tacctcatcc cgttccatcc gctcctggcg ttcgcggcgg gcgctctcgc
     2161 cgcctcgccg ccttcgcccg ggcaatggcc cgcacggatg cggctcgcgg ccgccgtcct
     2221 cgcgatcgcg ttcctcggga acgccgcgta cgacgtgtgg aagaccgacg gggcactgcg
     2281 gaaggtcacg cagccgggcg ggtcccggct ccgcccgcct tacatcttcg cgcggtagag
     2341 gcgtctctat tccggaaccg cgggaacgaa cgatccgtcg tccgtgccct ggcctggctc
     2401 cgcgccgtcg ttgatgactc cgtatgcggt gaaagcgggg ggcgcgtact ccacgggcag
     2461 ggaaggaatg atccgcgccc atccgcgcgc caggcccggc gcaacattcc ggaggatcgc
     2521 gttcatctgg aggacgccgg attcgacgtg tacgccgtcc cggacgcccg cgagcgcgcc
     2581 cgaggggtcc cggatctcga cgcggaaatc gatgaacccg aaaccgctac cgggggagac
     2641 gccgacgttg ctccgcgttc ccgcgtcctg gcgcaggtcg ggaatgacga cggaatcggc
     2701 ccggcccacg tatcgcgccg actcgaaaac tccgtacgac cgccccggcg cgggagacga
     2761 cgagacgcgc acacccgccg cgatgaaccc ggacgcagag agggaaacgg acgacacgat
     2821 ttccacctcg gccggcggag cgcccgggac gcctcgcctc cggaattcgg cgaacagatc
     2881 gggcacgtaa gacgtctcac cggccgagat caaaagcgtg aacgaggggc cctggaatgg
     2941 atctgcgccg aggcgcacga cgagccccag tggagagtca cccgggttcg tcgcgaccaa
     3001 ctccgtcgtg tagcgcgcgg tctggacggc gttcggaatc tcgcgagtct ccgccctatc
     3061 ctcctccacc accccggcca cgaacgatcc gtcgctcgtt ccggcgtcgt tcacgacggc
     3121 ccatgagagg tagggcgcgc cgcccgacac cctctccacg cgcgcccagc cgaccgacgc
     3181 tgcgaggccc gcggtccgaa ggatgcgacc gaactggaag aagccgccgg gcggaagggt
     3241 gacgtccgga agaaccgccg gagactccgc gcccgccgtt cccgggtgga cggtgacgcg
     3301 cagcgtgatc gggccctcgg cgtcgccccc ggcgttcacg agcgccaggt tcgtgcggtc
     3361 ccgtgcgtcc tccttgagcc acggaacgat cgccgggccc cggagcgcgg aagcgagcgg
     3421 gactccgccg caggagacgc ccgcacccga gggcgacgtc gtccgcaccg aggcggtgac
     3481 ggctcgcggg gaagaggcgc ccgttacgtc gagccgcagc gtcccgacga cgttgcccac
     3541 gccgatcggc aacccctcgt cccgaaggaa cttgaccgcc gacggcgcga tcagctgagc
     3601 gcccgccccg aggtccttct cgacgacgcc gcttccgccg ccatcggccg ccgcgtagcg
     3661 caggatcacg tgggcgggcg tcgagcccga gttcgtgagg agcaggtccg agtcgaatcg
     3721 ggacccgaag agtcccgtcg tcgagacgac gaccggcacg atcgagcgat ccgtgcgcgc
     3781 accggcacgt gcgaggacaa caattccctc gcgtgtcgaa aggaagaacg tggctccggc
     3841 agccgggtac cggagcgccg caaccgaata aatctccagg agcggcgaga acaacggcag
     3901 gacgaggcgt tccccctcgg gtggaccgtc ggctcgacag acgacgaggg aatagtggtc
     3961 cgcccggaaa aggtcgacga gctcgtttcg cccgtccccg tccacgtcca gcgcgctcgg
     4021 ggtcaccaac cccgggactg ccagacaccc gcccgcccct ctgaattgcc catgcccgtc
     4081 gttcgcgtaa atgatgagcg tgtcgggtcc gatatagacc gagttcgggt atgcgaggtc
     4141 cagccggccg gttcccgtga ggtcggcgag cagcacacgg ccggggcttc ggcccgggag
     4201 cgagaccgac gtgacgcgga acgtcccgtc gcctgcgccg agccagacgg acgggtagcc
     4261 ccgatacatg cccgagagaa ctacgtcggc ccggccgtcc ccatcgaggt ctccagccga
     4321 cacggttccc gcgccgccgt aggtcgtgat cggcgtgacg aacggtccgt cgaatcgaag
     4381 cccgcccctc gacaggaaag actggatcgt cactccgtcg accaccacga ggtccggccg
     4441 tccgtccccg tcgacgtcgg ccgcagaaag gtcgttgaac gaaccggacc gtgcgagcga
     4501 gagcgatggc gcgaacgaac cctttccgtc gcctttcagg atgtcaattc ctgcgcctcc
     4561 cgcggccgcg agatccagaa atccgtcgcc gtcgaaatca ccgaacgtga actgtcggta
     4621 ggtctcggga atggcggtca ccacgggctc ctgcgaccca tcgaggagcc agacgttcac
     4681 gaatccgcgg ccgacactga cgagcgaggg ggagccgccc ttcgtgaggg tttggacgga
     4741 cagcgccgtc gggggtccga cgcggccgag ctcggcgacg accttcaggt cctgagcgta
     4801 cgcgggggcg ccgccgagaa gggcgacgac caaggcaaac cgcgggaccg ggagcatctt
     4861 ccctgggaag tgtcgctccg tgaaccgcat ctcacaaaga aaaacgggcg ccgaagcgcc
     4921 cgttcccaag ttgcacgatg acgttgggtc tcagtcgacc tgcgccttcg cgtgcttctt
     4981 ctcggcgagc gcgcgcaccg tggcgccggg cagcgtgccg gacaccgaga cggtcgaccg
     5041 gtcgcgcgtc acggcgaact tgcggaggac ggccaccgtg tccggcgact tctcctgcgc
     5101 ggcgaggcgc caggctgcga ggacgccgcg gagcgcgtcc tccatgaggc cggccgtctc
     5161 gtcgtcggac gcgacgcccg tcgcgttcag cttcagcgcg tcgcccttcg ccgtcgcctg
     5221 gagcgcgagg agcgagacgt tcttcatcga ggcgacgagc gtcgcggccg cgtccattcc
     5281 cttcccctcg gcctgtacgt gcgcgtgccg catggcggcg tcctttgccg ggatgcgcgt
     5341 cacgtccacg agcgcccagg cggacgcgcc ggaatcgatg cggccgaggt ggcgcccgag
     5401 gcccgcgccc gagcggaagg ccgtgccgcc cgcgctcctc tgtgcgagcg ccgcgacgac
     5461 ggacgcctcg ttgcccgcga tgacgaggtg gttgctcgcg aacgcgatcg cgcccggttc
     5521 gtgccgcgcg gcgttctgcc ccttgtcggg aagcaggaag tagtcgcccg cgggcgtgga
     5581 cttccgctcg gcgccgcgcg cggcgatcgc ggacgagagg gcggcgacgt cgaagcggcc
     5641 ctcgaagagg acgaggcccc agcctgcgct gccgccggcg cccttgggcg atccggccgc
     5701 gacgaccgtg tcgacgtcct ccttcgggtt caggcgcgcc tcggcgagga agtgcgcggc
     5761 gtcgccgtcc accgcgatcc ggtccgtgtc cgagaagacg cgcgccgcga gcgggctcgt
     5821 gcgcaggtcc gcgacgtgga tgacgcccac ggaggccgcg tcggccggaa cgagcgcgag
     5881 cgcgctgtcg gtggccgcgg cggccgggag cgcgagcgtg gaggcgaggg cgaggacggc
     5941 ggcggaggcg gcgattcgct tcatggtcag aagtcctttc tccggaaacg gaggcacgcg
     6001 agggcgaggc atgcggcgcc gaacgcgccg gtcgtgagga acggctggag ggcgagggcc
     6061 ttctggatgc gcgccgggcc ctggtcgccc atcacgaggg cgacgacggc ggtgccgagc
     6121 tcggcagtct tcggaaagac ccagtagagg ccctggacga gctgcgcggc ccactcgtac
     6181 gacatcgcgg ccgcgatgcg gtcgtggttc acgaggatgg ccgagaggaa gaagagtgcg
     6241 aacgtggcca tgatggaaac ggccgtcgag gacgtgagcg tccccacgag gaacgcgaac
     6301 gccatgagcg ccgcgatccc gaacaggatg atccccgccg cgaggaagaa cctcgggtgg
     6361 acgaggcgcg tcttccagat gacgaggagc cacatcgacc cgaggagata gaggacgttc
     6421 gtcgccgaga ggaggacgcc gccgagatag cgggagagga ggagcggcac ccggccgacc
     6481 ggccgcgaga ggtagaggtc gaccgtgccc ttctccatga ggcgcggcac gaggtgcgcg
     6541 gtcgcgaaga tcgcgaggaa cgtcccgacg acgtagagga agccggcgaa gccggactcg
     6601 aagccgagga cgagcttgtc gaggtccacg gactgccggc ccatgtggac gttcgccccg
     6661 aagatcttcg cgcccgcgag cgcgccgttg acgacgtcga ggttcaccgc gacggcgaag
     6721 aggaggatga agagcgacga gagggcaaag tacgcgacga gcgtccagcg cgccgccgct
     6781 tcgcggaaga cctcttcgat gttggcggcc agtgctcgca tgtctgctct tctctagttc
     6841 gtaaggccgt ttgaggattt cttagaaggt gaagagggcg gctccgcgtc gggggtcgcc
     6901 ttgacgagat ccacgaacac gtcctcgagc gtggatttct ccggagtgag ctcggtcagc
     6961 agcgcgccgc gctggcgtgc ggcgtcgatg agcgaattca gatgctgtgc gtcgcgggcg
     7021 aggacgcgca catgcccgtt cacgcgctcc gggttcgctc ccctttcgaa gaagtccgaa
     7081 aggagctcct cggaaagagg cgaagccacc agcttgtacc ctttcaaaga agtcttctct
     7141 tcgtctttct tcgtcaattc ctccaccgtc cccatcgccg cgaccttgcc cttgcggagc
     7201 accgccacgc ggtcgcaggt cagctcgatc tcggagagga ggtgggagtt gaggaggatc
     7261 gcgcggccgc gggaggcttc cgcgcgcagg aggtcgcgga tctcgcgccg gcccacgggg
     7321 tcgacgccgt ccgtcggctc gtcgaggagg aggacctcgg gttcgtggac gagcgcagca
     7381 gccaggccga gccgctgcgt catgcccttg gagaacttct tcaccttcac gtccatccag
     7441 tcgccgagct tcacgcgctc gagaaggccc gggatgcggg ccttcagcgt cgcgtggtcg
     7501 acgccggaca tgcgcccgaa aaccgacagc gtctggcgcg
//
LOCUS       DAOMFY010000192         7522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3093_length_7522_cov_6.798313, whole genome shotgun sequence.
ACCESSION   DAOMFY010000192 DAOMFY010000000
VERSION     DAOMFY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3093_length_7522_cov_6.798313"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2150)
                     /gene="pnp"
                     /locus_tag="PLB01_13905"
     CDS             complement(<1..2150)
                     /gene="pnp"
                     /locus_tag="PLB01_13905"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQQ78443.1"
                     /translation="MHDTLTVDLDGRLLTIETGKIAKQSDGAAVVKCGDTVVLVTACY
                     TKESKDIDFLPLTVEYKEYQYAAGRIPGGFFKREGRPTEKEILTCRMIDRPFRPLFPE
                     GFNHETQIVGLVLSADGENDPDVLAINGAAAALAINTIPFQHVLGAVRVGRIGDRWVF
                     NPTAKERKESDLDLVVVGTHDAVSMVEAGAKEVSEAVMLEAILRGHAQVRKIVEAIAA
                     MAARRGVVKAPFTSPAPVPAEFAAAVRKQWEGPMMEALTHVGKIESYAKIKEVKKYGV
                     ELIPEDQPEARKMAKRALSEMVETLTRETILKQRKRLDGRAFDQVRPITIEAGVLPRT
                     HGSSLFTRGETQALVTVTLGTSDDTQLIEDLEGESERAFLLHYNFPPFSVGEVKRFGS
                     PGRREIGHGRLAWRSLQAVLPDEKSFPYTIRVVSDILESNGSSSMATICGGSIALMDA
                     GVPVKGAVAGVAMGLVKEGDAFAILTDIAGQEDHYGDMDFKVAGTRKGITALQMDIKI
                     SGITPQIFEQALEQARHGRLFILDKMEQTLHNARPELSAFAPRLLTISVPEDKIRDII
                     GSGGKTIRALQKEYSCKIDVQDDGTVTVASSDMPTAEKCLEAIRQLTTVPEIGTEYLG
                     TVVRIEPYGCFVQILPGMDGLVHISELAPGRVRETTDVVKMGDKIKVKIIAIDPVNGK
                     VKLSRRQALTPEESAAEVERLGLTAVAAGEGGGD"
     gene            complement(2259..2528)
                     /gene="rpsO"
                     /locus_tag="PLB01_13910"
     CDS             complement(2259..2528)
                     /gene="rpsO"
                     /locus_tag="PLB01_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQQ78444.1"
                     /translation="MAQLTTKKAETITTYRRHDSDTGSPEVQVALLSKRISELTEHFK
                     AHAKDHHSRRGLLQMVGRRRRLLDYVKKRDADRYRALIERLGLRK"
     gene            complement(2619..3545)
                     /gene="truB"
                     /locus_tag="PLB01_13915"
     CDS             complement(2619..3545)
                     /gene="truB"
                     /locus_tag="PLB01_13915"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQQ78445.1"
                     /translation="MSVRPAGPDGVLLVDKPEKLTSHDVVDVARRLLGTRRIGHTGTL
                     DPAATGLLVLCVGRAGRLQSFLTGWDKTYEGTIVFGVATDTYDTEGAPAGAPHAGPAP
                     SRGALEDACRAFTGEILQVPPPFSAKKIGGKKFYELARAGEPVPDTPKKVTVRSLEIL
                     GLDGAVARFRVACTTGTYIRSLAHDIGKALGTGAHLGSLRRTSIGPFRLEDARTLPAL
                     EALPREERFGKPSWMSLSEIPLPFPVVALAPAEAAKVKSGHGVPVRVPEGAAGAAMVR
                     LTDGGELLALGILEPLGRGALALARPKVVLAG"
     gene            complement(3542..4552)
                     /locus_tag="PLB01_13920"
     CDS             complement(3542..4552)
                     /locus_tag="PLB01_13920"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HQQ78446.1"
                     /translation="MTRDAALDEAYVAVADLLRNGRRFLLTGHRNPDGDALGSALGLA
                     QALEAAGKEARVVMRDPWSYAYDGMPGISRVEVLEALPADWPAAYDGILVMECPESDR
                     TGWPNLFAGKVVNIDHHPGNARYGALNLIDLPAAAVGEIVADLLDLLQWPLTADAATN
                     LWVSLVSDTGSFRYSNTTPKALALGARLVAAGAQPGPVNEFLFEAAPLSSLKLEALVL
                     GTLALHAGGAVATVELPRRFFTESGAQESDTEGLVNRARGIHGVKAAALLREGEAGEI
                     RCSLRSKGTVDVRSVAARHGGGGHRNAAGCRIKGTLDAAKAALVAEIAAAVAADSVPE
                     AR"
     gene            complement(4549..4947)
                     /gene="rbfA"
                     /locus_tag="PLB01_13925"
     CDS             complement(4549..4947)
                     /gene="rbfA"
                     /locus_tag="PLB01_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQQ78447.1"
                     /translation="MVREKKAGPPSRRPKQVGDVVRAELARLLREELRDPAIGFATIT
                     DVVMADDLRSARVHVSVFGAKDQFAKTVAALNHARGHLRSLVGRNCGLRYAPELHFVE
                     DHTLERGARIEELLRTIPKPGGADDPGDTE"
     gene            complement(4949..5290)
                     /locus_tag="PLB01_13930"
     CDS             complement(4949..5290)
                     /locus_tag="PLB01_13930"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HQQ78448.1"
                     /translation="MVVAIAVIDFHLPGCRGLKEKRAFLRPLKARLRGEFEISAAEVA
                     HQDLLQRAVLGVAAVGPDREALEPLLQRVLGYVEGYAEENGAEIAAVHHEFTNYGDVG
                     AAGNAFGASGD"
     gene            complement(5368..>7522)
                     /gene="infB"
                     /locus_tag="PLB01_13935"
     CDS             complement(5368..>7522)
                     /gene="infB"
                     /locus_tag="PLB01_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQQ78449.1"
                     /translation="PGFGRPPLGGPAAPPLPDTADRRKKDATKPGLAVKKGDAKKAES
                     KKPRTKEVSALEEDVREYLGSYKQDTYEDIPTPSAEGTDEALAAKPLSKSAQRRAGKK
                     TMATETGEVVEVKKKAEGPVFLSEGVTIKELSEKLGVLAKDLMKSLLARGIFATINQP
                     VEQKLAVEIAKEFGVEAAVVSFEEELELSRQQTAIDSAAEAQDASAGDTSGGVRVPRA
                     PVVTVMGHVDHGKTSLLDAIRKENVAEGEAGGITQHIGAYRVEVKGKTIVFLDTPGHE
                     AFTLMRSRGAKATDIVVLVVAADDGVMPQTAEAIDHARAAKVPIVVAINKIDKPDANV
                     QRVKQELADKGVLVEEFGGDVVACEISAKKKIGIDQLLEMILLQAEVLELKAVATGHA
                     RGVVLESRREAGKGTLATVLVQQGTLRVGDVFFAGAAVGRVRAMLNDRGARLTEAGPA
                     TPVEVMGFDDLPDAGDTLQVVEDESKARQVSSFRSQKEREESLAKSSRMSLDSLFNRM
                     KEGVAKDLALIVKADVHGSEEVLVQTLGKLPSDKVKVSILHSGVGAISVNDVLLAAAS
                     EAIIIGFNVKPEKKAQDLADHEGVEIRLYSVIYTLTDDIKKSMAGLLDTVKKDVTRGH
                     AEVREIFKVPKIGTVAGCMVTDGSIPRSASVRLVRDHRVIYEGKIGSLRRFKDDASEV
                     KQGFECGIGIAGYQDVKVGDVIEAFVVEEMAASLT"
BASE COUNT         1013 a         2559 c         2707 g         1243 t
ORIGIN      
        1 tcgtcgccgc cgccctcgcc ggccgcgacg gccgtgaggc cgaggcgctc gacttcggcg
       61 gcgctctctt cgggcgtcag ggcctgacgg cgcgagagct tgaccttccc gttgaccggg
      121 tcgatcgcga tgatcttgac cttgatcttg tcgcccatct tgacgacgtc ggtcgtctcg
      181 cggacgcggc cgggcgcgag ctcggagatg tggacgaggc cgtccatgcc cgggaggatc
      241 tgcacgaagc agccgtaggg ctcgatgcgc acgaccgtgc cgaggtactc ggtgccgatc
      301 tcggggaccg tcgtgagctg gcggatcgcc tcgaggcact tctcggcggt cggcatgtcg
      361 ctggaggcga ccgtgacggt gccgtcgtcc tgcacatcga tcttgcagga gtactccttc
      421 tggagcgcgc ggatcgtctt gccgccggag ccgatgatgt cgcggatctt gtcctcgggg
      481 accgagatcg tgaggaggcg cggcgcgaag gccgagagct cggggcgcgc gttgtgcagc
      541 gtctgctcca tcttgtcgag gatgaagaga cgcccgtggc gggcctgctc gagcgcctgc
      601 tcgaagatct gcggcgtgat gccggagatc ttgatgtcca tctggagggc cgtgatgccc
      661 ttgcgcgtgc cggcgacctt gaagtccatg tcgccgtagt ggtcttcctg gccggcgatg
      721 tccgtgagga tcgcgaacgc gtcgccttcc ttcacgaggc ccatcgcgac gcctgcgacc
      781 gcgcccttga cgggcacgcc ggcgtccatg agggcgatcg agccgccgca gatcgtcgcc
      841 atcgacgacg agccgttgga ctcgaggatg tcggacacga cgcggatcgt gtacgggaag
      901 gacttctcgt cgggcaggac ggcctgcagc gagcgccagg cgaggcggcc gtggccgatc
      961 tcgcggcggc ccggggagcc gaagcgcttc acctcgccga ccgagaacgg cgggaagttg
     1021 tagtggagca ggaaggcgcg ctcggactcg ccctcgaggt cctcgatgag ctgcgtgtcg
     1081 tcggaggtgc cgagcgtgac ggtcacgagc gcctgcgtct cgccgcgggt gaagagcgac
     1141 gagccgtgcg tgcggggaag aacgcccgcc tcgatcgtga tcgggcggac ctggtcgaac
     1201 gcgcggccgt cgaggcgctt cctctgcttg aggatggtct cgcgcgtgag cgtctcgacc
     1261 atctccgaga gggcgcgctt ggccatcttg cgggcctcgg gctggtcctc ggggatcagc
     1321 tcgacgccgt acttcttgac ttccttgatc ttcgcgtacg actcgatctt tccgacgtgg
     1381 gtcagggcct ccatcatcgg gccctcccac tgcttgcgga cggcggctgc gaactcggcc
     1441 ggcaccgggg cgggcgacgt gaacggcgcc ttgacgactc cgcggcgggc ggccatcgcg
     1501 gcgatggcct cgacgatctt ccggacctgc gcgtggccgc gaaggatcgc ctcgagcatc
     1561 acggcctcgg agacttcctt cgcgcccgcc tcgaccatcg agacggcgtc gtgcgtgccg
     1621 acgacgacga ggtcgaggtc ggattccttg cgctccttcg cggtggggtt gaagacccac
     1681 cggtcgccga tccggccgac gcggaccgcg ccgaggacgt gctggaacgg aatcgtgttg
     1741 atcgcgagcg cggcggcggc gccgttgatc gcgaggacgt cggggtcgtt ctcgccgtcg
     1801 gccgagagga cgaggcccac gatctgggtc tcgtggttga agccctcggg gaagagcggg
     1861 cggaacggcc ggtcgatcat ccggcaggtc aggatttcct tctcggtcgg gcgcccctcg
     1921 cgcttgaaga agccgccggg gatccggccc gccgcgtact ggtattcctt gtactcgacg
     1981 gtgagcggga ggaagtcgat gtccttcgac tccttcgtgt agcaggcggt cacgaggacg
     2041 accgtgtcgc cgcacttcac gacggcggcg ccgtcggact gcttggcgat cttgccggtc
     2101 tcgatcgtga gtagacgtcc atccaggtcg acggtcaggg tgtcatgcat gggcatttcc
     2161 tttcggtggc ccggcgcgcc gtccggaaag cacgtctcac tcgggccgcg gggtgcgggc
     2221 cgggaagtgc cgccggacga ttgccgtgaa gaaaaggact acttgcggag tccgaggcgc
     2281 tcgatcaacg cgcggtaccg gtccgcgtcg cgcttcttca cgtaatcgag caggcggcgg
     2341 cgccggccga ccatctggag gagacccctc cgggaatggt ggtccttggc gtgggccttg
     2401 aagtgctcgg tcagctcgga aatgcgcttg gacaggagag ccacctggac ctcgggcgaa
     2461 cccgtgtcgc tgtcgtggcg ccggtacgtc gtgatcgttt ccgccttctt cgtggtgagc
     2521 tgggccaatg tcttatcgtt ccttctctaa tttttcagaa cggggagtct agccgaccgg
     2581 ccggaacggg tcaaccaacc gggaagcggg cgagagcgtc atcccgcgag gacgactttc
     2641 ggccgggcga gggcgagggc gccccgtccg aggggctcga ggattccgag ggcgagaagc
     2701 tcgccgccgt ccgtgaggcg gaccatcgcg gccccggccg ccccctcggg cacccgcacg
     2761 ggcacgccgt ggccgctctt cactttcgcg gcctccgcgg gcgcgagcgc cacgaccggg
     2821 aagggcagcg ggatctccga gagcgacatc cacgacggct tcccgaaacg ctcctcgcgc
     2881 gggagagctt ccagcgccgg cagcgtgcgc gcgtcctcga ggcggaacgg cccgatcgac
     2941 gtgcgccgca ggctgccgag gtgcgcgccg gtcccgaggg ccttcccgat gtcgtgggcg
     3001 agcgagcgga tgtacgtgcc ggtcgtgcac gcgacgcgga agcgcgccac ggcgccgtcg
     3061 agcccgagga tctcgagcga gcggacggtc accttcttcg gcgtgtccgg caccggctcc
     3121 ccggcgcgcg cgagctcgta gaacttcttc ccgccgatct tcttggccga gaacggcggc
     3181 ggcacctgca ggatctcgcc cgtgaacgcg cggcaggcgt cctcgagcgc gccccgggac
     3241 ggggccgggc ccgcgtgcgg cgcgccggcg ggcgcgccct cggtgtcgta ggtgtcggtc
     3301 gcgacgccga acacgatcgt gccctcgtac gtcttgtccc agccggtcag gaacgactgg
     3361 agccggcccg cccggcccac gcacaggacg agcaggcccg tcgcggccgg gtcgagcgtg
     3421 cccgtgtggc cgatgcggcg cgtgcccagg agccggcgcg cgacgtcgac gacgtcgtgc
     3481 gacgtgagct tctccggctt gtcgacgagg aggactccgt cggggcccgc cgggcgaacg
     3541 ctcaacgcgc ctccggaacg gagtcggccg cgacggccgc ggcgatctcc gcgacgagcg
     3601 ccgccttcgc ggcgtcgagc gtgcccttga tccggcagcc ggccgcgttg cggtggccgc
     3661 ccccgccgtg ccgcgccgcg acgctccgca cgtccacggt ccccttgctc cgcagcgaac
     3721 agcggatctc cccggcctcg ccctcgcgca ggagcgcggc ggccttgacg ccgtggatcc
     3781 cgcgcgcgcg gttcacgagg ccttccgtgt cggattcctg cgctccgctc tcggtgaaga
     3841 agcggcgggg aagctcgacg gtcgcgacgg cgcctcccgc gtggagcgcg agcgtgccga
     3901 ggacgagcgc ctcgagcttc agcgacgaca gcggcgccgc ctcgaagagg aactcgttca
     3961 cggggcccgg ctgcgcgccg gccgccacga gccgcgcgcc gagcgcgagg gccttcggag
     4021 tcgtgttcga gtagcggaac gagcccgtgt ccgacacgag cgagacccac aggttcgtcg
     4081 cggcgtcggc cgtgagcggc cactggagga ggtcgaggag gtccgcgacg atctcgccaa
     4141 cggcggccgc ggggaggtcg atcaggttca gcgcgccgta gcgcgcgttg ccgggatggt
     4201 ggtcgatgtt gacgactttc cccgcgaaca ggttcggcca gcccgtgcgg tccgactccg
     4261 ggcactccat gacgaggatc ccgtcgtacg ccgccggcca gtcggcgggc agcgcctcga
     4321 gcacctcgac gcgcgagatc cccggcatcc cgtcgtaggc gtacgaccac gggtcgcgca
     4381 tgacgacgcg cgcctccttg cccgccgcct cgagcgcctg cgcgaggccg agcgccgagc
     4441 cgagggcgtc gccgtccggg ttgcggtggc ccgtgaggag gaagcggcgc ccgttgcgga
     4501 ggaggtccgc gaccgcgacg tacgcctcgt cgagcgcggc gtcgcgcgtc attcggtgtc
     4561 gccggggtcg tcggccccgc cgggcttcgg gatcgtgcgg agaagctcct cgatgcgcgc
     4621 gccgcgctcg agcgtgtggt cctcgacgaa gtggagctcc ggcgcgtagc gcaggccgca
     4681 gttgcggccg acgagcgagc gcaggtgtcc gcgcgcgtgg ttgagcgccg cgacggtctt
     4741 cgcgaactgg tccttggccc cgaacacgga gacgtggacg cgcgccgagc gcaggtcgtc
     4801 ggccatgacg acgtccgtga tggtcgcgaa cccgatggcg ggatccctca gctcttcccg
     4861 gaggaggcgc gcgagctccg cgcgcacgac gtcgccgacc tgcttcgggc ggcggctggg
     4921 cggcccggct tttttctctc gaaccatgtc agtctcccga ggccccgaac gcgtttccgg
     4981 cggctccgac gtcgccgtaa ttggtgaatt cgtgatgcac cgccgcgatc tcggcgccgt
     5041 tctcttccgc gtatccctcg acgtacccga gcacgcgctg gagcaggggc tcgagcgcct
     5101 cccggtccgg cccgaccgcc gcgacgccga gcaccgcgcg ctggaggagg tcctggtgcg
     5161 cgacctccgc ggccgagatc tcgaactcgc ccctcagccg cgccttgagc gggcgaagga
     5221 acgccctttt ttccttcagg ccgcggcacc ccggcaaatg gaaatcgatt actgcgattg
     5281 caacgaccat tgaagatttc ttagaagggt aagaggggca ggcggcgatt cgccgcccgc
     5341 tctcccttca ttcgaagaaa atccctctta ggtaagcgaa gcggccatct cctcgacgac
     5401 gaacgcctcg atcacgtcgc ccaccttcac gtcctggtag ccggcgatgc cgatgccgca
     5461 ctcgaagccc tgcttgacct cggaggcgtc gtccttgaag cgcctgagcg agccgatctt
     5521 cccctcgtag atgacgcgat ggtcgcgcac gaggcggacg ctcgcggacc gggggatcga
     5581 cccgtcggtg accatgcagc ccgcgaccgt cccgatcttc ggcaccttga agatctcgcg
     5641 cacctcggcg tggccgcggg tgacgtcctt cttgacggtg tcgaggaggc cggccatgga
     5701 cttcttgatg tcgtccgtga gcgtgtagat gacggagtag agccggatct cgacgccctc
     5761 gtggtcggcg aggtcctggg ccttcttctc gggcttcacg ttgaagccga tgatgatggc
     5821 ctcggacgcg gccgcgagga gcacgtcgtt cacggagatc gcgccgacgc ccgagtggag
     5881 gatcgagacc ttcaccttgt ccgacgggag cttgccgagc gtctggacga ggacctcctc
     5941 ggacccgtgg acgtccgcct tcacgatcag cgcgaggtcc ttggcgacgc cctccttcat
     6001 gcggttgaag agcgagtcga gcgacatgcg cgaggacttc gcgagcgact cctcgcgctc
     6061 cttctgggag cggaacgagg agacctgccg cgccttcgat tcgtcctcga ccacctgcag
     6121 cgtgtcgccg gcgtccggaa ggtcgtcgaa gcccatgacc tcgacgggcg tcgcggggcc
     6181 ggcctcggtg aggcgcgcgc cgcggtcgtt gagcatcgcg cggacgcggc cgacggccgc
     6241 gcccgcgaag aacacgtcgc cgacgcggag cgtgccctgc tggacgagaa cggtcgcgag
     6301 cgtgcccttg ccggcctcgc ggcgcgactc gaggacgacg ccgcgggcgt ggcccgtcgc
     6361 gacggccttg agctcgagca cctcggcctg caggaggatc atctcgagga gctggtcgat
     6421 tccgatcttc ttcttcgcgg agatctcgca ggccacgacg tcgccgccga actcctcgac
     6481 gaggacgccc ttgtccgcga gctcctgctt gacgcgctgg acgttcgcgt cgggcttgtc
     6541 gatcttgttg atcgcgacga cgatcgggac cttcgcggcg cgcgcgtggt cgatcgcctc
     6601 ggccgtctgc ggcatgacgc cgtcgtcggc cgcgacgacg aggacgacga tgtccgtcgc
     6661 cttggcgccg cgcgagcgca tgagcgtgaa ggcttcgtgg ccgggcgtgt cgaggaagac
     6721 gatcgtcttg cccttgacct cgacgcggta ggcgccgatg tgctgggtga tgccgcccgc
     6781 ctccccctcg gcgacgttct ccttgcggat cgcgtcgagg agcgacgtct tgccgtggtc
     6841 gacgtggccc atgacggtga cgacgggggc ccgcggcacg cgcacgccgc ccgaggtgtc
     6901 gcccgcgctc gcgtcctgcg cctcggccgc ggagtcgatg gccgtctgct ggcgggagag
     6961 ctcgagctcc tcctcgaacg acacgacggc cgcctcgacg ccgaactcct tcgcgatctc
     7021 gaccgcgagc ttctgctcga ccggctggtt gatcgtcgcg aagatcccgc gcgcgagaag
     7081 ggacttcatg aggtcctttg cgaggacgcc cagcttctcg gacagctcct tgatcgtgac
     7141 gccttccgag aggaagacgg gcccctcggc cttcttcttg acctcgacga cctcgccggt
     7201 ctcggtggcc atcgtcttct tgccggcgcg gcgctgcgcc gacttggaga gcggcttggc
     7261 ggcgagcgcc tcgtccgtgc cctcggctga cggcgtcggg atgtcctcgt aggtgtcctg
     7321 cttgtagctg ccgaggtact cccgcacgtc ttcttcgagc gcggagacct ccttcgtgcg
     7381 cggcttcttg gactcggcct tcttcgcgtc gcccttcttg acggcgaggc cgggcttcgt
     7441 cgcgtccttc ttccgccggt ccgccgtgtc gggcagcgga ggcgcggccg ggccgccgag
     7501 cggcgggcgt ccgaagccgg gg
//
LOCUS       DAOMFY010000193         7500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3108_length_7500_cov_6.375285, whole genome shotgun sequence.
ACCESSION   DAOMFY010000193 DAOMFY010000000
VERSION     DAOMFY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3108_length_7500_cov_6.375285"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLB01_13940"
     CDS             complement(<1..1184)
                     /locus_tag="PLB01_13940"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HQQ78450.1"
                     /translation="MSTKKLLVLTGVFVALLAFVVLFESKQPTSTERAKAAKRLADFK
                     ADDVVSVTLERPDLPKVELVRKDKGRWTVSNAPASAADGFGAEALVADLGRLEVVGET
                     RTAFDPKELGLDAPKARATLAFLDKSTKVISFGAPIPGTDAVAAAAGPVFGAVRQAPL
                     AALTKPVDEYRSKQLFDVPVGDVTRVAVTRGPNTVVVARGAKTPSGAPGGWRLEKPVA
                     DLASDAFVDRLLGDLAGARVLEFPSLTDADLPRVGLAPAVATVVLEKGAEKVATIGFG
                     AEKAEGSGGRMYAKVGSLVVVIDDRVREDLDKELSAYRESRVAPVDTFTLRRVSFSSD
                     DLRAGADRVDGAWRSAGKPVTALHAEGLAAVLGRAEGRGFVPARPGKPAPEKALATVD
                     LAG"
     gene            complement(1189..2670)
                     /locus_tag="PLB01_13945"
     CDS             complement(1189..2670)
                     /locus_tag="PLB01_13945"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HQQ78451.1"
                     /translation="MTAVKTPPTRNTFRATLALSVVLGLGILVLVNYIGSRRYARFDW
                     TGSGLYSLSEKTRNVLRDLKAPVQVTVFMTPGTPLYPEVDELLKRYKAASPMLTVETL
                     DPTRNLVRAKQLVDEFGVKSSTVVFRSGERKKYVTGEQLAEMDYSRARMGGEPGIKAF
                     KGEQEFTSAILAVTQQKTPKVVFTTGHGERRFDAPRARDGFSEAADSFKNDNCTVEEW
                     ASLGAADVPAGTDLVVVAGPRTAFTEPERAALKRYFDAGGRALFLLDVELTPGSPALS
                     DFGLNPLLGAMGLTLDSDVVLDPKNALPLMGPETVFAKSFRPHAVTRLLTGSAVVFPL
                     ARSVSVAASPAPGWTATPLVETSPDGWGETDIRELQKTGRPAKDEKDVKGPVCLVAAA
                     ESAPAADKAESKKRARVVVFGDSDWVSNAGIANAANRLLLTSAANWALEREALVAIPP
                     KNADQVAVTLSRRDVGLIAFFVLLLLPVAAVGLGLAIWVRRRR"
     gene            complement(2672..3418)
                     /locus_tag="PLB01_13950"
     CDS             complement(2672..3418)
                     /locus_tag="PLB01_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78452.1"
                     /translation="MSALKAFTAIVKREFFSYFVSPLAYVVLTAFLLIQGYTFYLIVL
                     ALNQPETPRTALMSLFFTSVFYWIFMMLVSSVIAMRLLSEERKTGSIEALLTAPVSEA
                     TVVAGKFAAGWCFFLFLWLPTVLYPVLLSRFGTLDWGPIASGYVGIALVGALFISAGT
                     FASALTKNQIVAAIVGFVFILGVFIVGVFRDFVTDPAYRDALSYLNLLEHMDDFSRGI
                     VDTRRLLYVASTVVFFLFLSTRALEANKGR"
     gene            complement(3415..4422)
                     /locus_tag="PLB01_13955"
     CDS             complement(3415..4422)
                     /locus_tag="PLB01_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ78453.1"
                     /translation="MLEVQGLSKSYYGTSAVEDVSFRVETGEILGFLGPNGAGKTTTM
                     RMITGFLPPTAGRVLLADMDVTEKPLEAKRELGYLPETLALYPEMRVREYVAFRAALC
                     GVPSAKRRVRVDEAIQKCFVDDVATKPIGNLSKGYRQRVGLAGALVHGPKVLVLDEPT
                     VGLDPRQIVKVRDLIRDLKKDHTILLSTHILPEVEVLCDRVLIIDRGRIVAAGTPDTL
                     RHEMSQAPAVTVVLKGEVSEADAVLRQIPGVTRAARIGAGGETRVSLAGAPGADLREP
                     VFREILTRGWTLLELTPRQASLEDVFVRLVSRDASEDADTQPGVDSSTDTPALGTEAP
                     Q"
     gene            4539..5867
                     /locus_tag="PLB01_13960"
     CDS             4539..5867
                     /locus_tag="PLB01_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HQQ78454.1"
                     /translation="MSQPVAKVVDLTDPTLTSLRGTSVEDARARVARGDAEGVAALDG
                     SYALVGVDGIAVRLARSMDRPLRYFLAKEAAGPVLVVADRIDAIARWLADHGHADQFH
                     PSYTRMVPAHHLATVRLVGCPDPEPSYVRTFTPQRNALPADLDAIGRLYVSALADEIA
                     GWIERVPAQAPLGVCFSGGIDSGAVFLVTHHVLRKLGMSPARLKAFTLTLGGGPDLAQ
                     ARAFLDTCDMSFFLEPVEADAASLDPFEAVRVIEDYKPLDVESATMAIALLRGIRARY
                     PEWTHLLDGDGGDENLKDYPIEENPELTIRSVVNNLMLYQEGWGVGSLKHSQTYTGGQ
                     SRSYARTYAPLRLFGFTGFSPFTRPRVVAVAEGIPFADLTQGSVEKLYALKGEIVGRG
                     VKAVTGLTMPVFPKRRFQHGAGGAAVHAQRFPYGEARYRRALIEAFAGAA"
     gene            5864..6856
                     /locus_tag="PLB01_13965"
     CDS             5864..6856
                     /locus_tag="PLB01_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ78455.1"
                     /translation="MSAVFPETRAERERFMAARRPAKNVLDPRRPYGFFVEDEPAAPG
                     VPVPVATILLTNRECTTRCTMCDLWKNTLDGPTPPGAIPEQIRWALERLPAARRIKLY
                     NAGSFFDRAAVPPEDHAAIAALVRGFERVVVECHPALAGDALLRFRDLLAGPELEVAV
                     GLETANEVALEKINKGMTVEEFEACTRRLVSRGLPVRSFALVGVPFLRKEEWAEATRA
                     SIDVSFRAGAEIVSLIPTRLGNGTLEELQRTGDFTPPALSDLERAMEDFFEGEEGQAG
                     ATGMLLADLWNARDLPAPACCASARIERLRAMNTFQRNLPLPPSLSPLPCPVCS"
     gene            6853..>7500
                     /locus_tag="PLB01_13970"
     CDS             6853..>7500
                     /locus_tag="PLB01_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ78456.1"
                     /translation="MNEAFDVAVVGSGFAGSLFALVARRAGRTVALLEKGAHPRFAIG
                     ESSSPLANLLLEEIAARFELPRLLPLTTWGTWRRTYPEITCGLKRGFTFYAHEEGRRF
                     AGAPDRSDQLLVAASPNDAVADTHWYRPDFDQFLVREAEAEGAAYRDRSRLAAVSREG
                     DGWRLEGDRLGEPFYRLEMPESGWMSGVRGAVLEAIGQVYFATATGIQMCEANGRV"
BASE COUNT         1101 a         2669 c         2656 g         1074 t
ORIGIN      
        1 cccgcgaggt cgaccgtcgc gagcgccttc tcgggcgcgg gcttccccgg cctggccggc
       61 acgaagccgc gtccctcggc ccggccgagc acggccgcga ggccctcggc gtggagcgcc
      121 gtcacgggct tgcccgccga ccgccacgcg ccgtcgaccc ggtccgcccc ggcccgcagg
      181 tcgtccgagg agaacgagac gcgccggagt gtgaacgtgt ccacgggagc gacgcggctc
      241 tcgcggtagg ccgacagctc cttgtcgagg tcttcgcgga cccggtcgtc gatcacgacg
      301 acgagggagc ccactttcgc gtacatccgt ccgcccgacc cctcggcttt ctcggcgccg
      361 aagccgatcg tcgcgacctt ttcggcccct ttctcgagga cgacggtcgc gacggccggc
      421 gcgaggccca cgcggggaag gtccgcgtcg gtgagcgacg ggaactccag gacccgcgcg
      481 cccgcgaggt cgccgaggag gcggtccacg aacgcgtcgg aggcgaggtc cgcgaccggt
      541 ttctcgagac gccacccgcc cggagcgccg gaaggcgtct tcgcgccgcg cgccacgacg
      601 accgtgttcg ggccgcgcgt cacggcgacg cgcgtgacgt cgccgacggg cacgtcgaag
      661 agctgcttgc tgcggtactc gtccaccggc ttcgtgagag ccgccagcgg cgcctgcctc
      721 accgcgccga agacgggtcc ggccgccgcc gccacggcgt ccgtgcccgg gatcggggcg
      781 ccgaaggaga tgaccttggt cgacttgtcg agaaaagcga gcgtcgccct ggccttcggg
      841 gcgtcgaggc ccagctcctt cgggtcgaat gccgtacgcg tttcgccgac gacctcgagc
      901 cgcccgaggt cggccacgag ggcctccgcc ccgaacccgt ccgccgcgga cgccggggcg
      961 ttcgagaccg tccagcgtcc cttgtccttg cgcacgagct cgaccttcgg caggtccggc
     1021 cgctcgagcg tcacggagac gacgtcgtcg gccttgaagt ccgcgaggcg cttggccgcc
     1081 ttcgcgcgct cggtcgacgt cggctgcttg ctctcgaaga ggacgacgaa ggcgagcaga
     1141 gcgacgaaga ccccggtcag gacgagcagc ttcttcgtgg acatcggact agcggcgcct
     1201 ccgcacccag atcgcgaggc ccaggccgac ggccgcgacg ggcaggagca ggaggacgaa
     1261 gaacgcgatc agcccgacgt cccgccgcga gagcgtcacg gcgacctggt ccgcgttctt
     1321 cggcgggatc gccacgagcg cctcgcgctc gagcgcccag ttcgcggccg acgtcaggag
     1381 caggcggttc gcggcgttcg cgatgccggc gttcgaaacc cagtcggagt ccccgaacac
     1441 cacgacacgc gcgcgcttct tcgattcggc cttgtccgcc gcgggcgccg actccgccgc
     1501 cgcgacgagg cacacgggcc ccttcacgtc cttctcgtcc ttcgcggggc ggcccgtctt
     1561 ctggagctcc cggatgtccg tctcgcccca gccgtccgga gacgtctcga cgagcggcgt
     1621 cgcggtccag ccgggagcgg gcgaggcggc cacgctcacg gaacgcgcga gcgggaagac
     1681 cacggccgac ccggtcagga ggcgcgtcac ggcgtgcggg cggaacgact tcgcgaagac
     1741 cgtctcgggc cccatgagcg ggagtgcgtt cttcgggtcg aggacgacgt cgctgtcgag
     1801 cgtcaggccc atcgcgccga gcagcgggtt gaggccgaag tcggagaggg ccgggctccc
     1861 gggcgtcagc tcgacgtcca gaaggaagag cgcgcgtccg ccggcgtcga agtagcgctt
     1921 cagggcggcg cgctcgggct ccgtgaacgc cgtgcgcggc cccgcgacga ccacgagatc
     1981 cgtgcccgcg gggacgtccg cggcgccgag ggacgcccac tcctccacgg tgcagttgtc
     2041 gttcttgaac gagtcggccg cctccgagaa gccgtcgcgc gcgcgcggcg cgtcgaaacg
     2101 ccgctcgccg tggcccgtcg tgaaaacgac cttcggggtc ttctgctgcg tgacggcgag
     2161 gatcgccgac gtgaactcct gctcgccctt gaacgccttg atcccgggct cgccgcccat
     2221 ccgggcgcgc gagtagtcca tctcggcgag ctgctccccc gtgacgtact tcttccgctc
     2281 cccggaccgg aagacgaccg tgctgctctt gacgccgaat tcgtcgacga gctgcttggc
     2341 cctcacgagg ttccgcgtcg ggtcgagcgt ctcgaccgtg agcatcgggc tcgccgcctt
     2401 gtaacgcttg aggagctcgt cgacctcggg gtagagcggc gtcccgggcg tcatgaacac
     2461 cgtgacctgg acgggggcct tcaggtctct gaggacgttg cgggtcttct ccgagaggga
     2521 atagagcccg ctccccgtcc agtcgaagcg cgcgtagcgg cgcgagccga tgtagttcac
     2581 gaggacgagg atcccgagcc cgaggacgac ggagagcgcg agcgtcgcgc ggaaggtgtt
     2641 gcgggtggga ggcgtcttca cggccgtcat ctcagcgccc cttgttcgcc tcgagggcgc
     2701 gcgtcgagag gaacaggaag aagacgacgg tcgacgcgac gtagaggagg cggcgcgtgt
     2761 cgacgatccc gcgcgagaag tcgtccatgt gctcgaggag gttcaggtac gacagggcgt
     2821 cgcggtacgc cgggtccgtc acgaagtcgc ggaagacgcc gacgatgaag acgccgagga
     2881 tgaagacgaa cccgacgatc gccgcgacga tctggttctt cgtgagggcc gacgcgaacg
     2941 tgcccgcgga gatgaagagg gcgcccacga gcgcgatgcc cacgtacccg gacgcgatcg
     3001 ggccccagtc gagcgtcccg aagcgcgaga ggaggaccgg gtagagcacc gtcgggagcc
     3061 acaggaagag gaagaagcac cagccggccg cgaacttgcc ggccacgacg gtggcctcgg
     3121 agacgggcgc ggtgaggagc gcctcgatcg agcccgtctt gcgctcctcc gagaggagcc
     3181 gcatcgcgat gacggacgag acgagcatca tgaagatcca gtagaagacg ctcgtgaaga
     3241 agagcgacat gagcgccgtc cgcggcgtct ccggctggtt cagcgcgagg acgatcaggt
     3301 agaacgtgta gccctggatg agcaggaacg ccgtcagcac gacgtaggcg aggggcgaga
     3361 cgaagtagga gaagaactct cgcttcacga tcgcggtgaa ggccttcagc gcgctcactg
     3421 gggagcctcc gttccgaggg cgggcgtgtc cgtgctcgag tcgacgcccg gctgggtgtc
     3481 cgcgtcctcg gaggcgtcgc gcgacacgag gcgcacgaag acgtcctcga gcgaggcctg
     3541 gcgcggcgtg agctcgagga gcgtccagcc gcgcgtgagg atctcgcgga agacgggttc
     3601 ccgcaggtcg gcgcccgggg cgcccgcgag cgagacgcgc gtctcgccgc ccgcgccgat
     3661 gcgcgccgcg cgcgtgacgc ccgggatctg gcgcagcacc gcgtcggctt ccgagacctc
     3721 gcccttcagg acgacggtga cggccggcgc ctgcgacatc tcgtggcgga gcgtgtccgg
     3781 cgtgcccgcg gcgacgatgc gcccgcggtc gatgatgagg acgcggtcgc agaggacctc
     3841 gacctcggga aggatgtgcg tcgagaggag gatcgtgtgg tccttcttca ggtcgcggat
     3901 caggtcgcgc accttgacga tctgcctcgg gtcgaggccg acggtgggct cgtcgaggac
     3961 gaggaccttc ggcccgtgga cgagcgcgcc cgcgagcccg acgcgctgcc ggtagccctt
     4021 cgacaggttg ccgatgggct tcgtcgcgac gtcgtccacg aagcacttct ggatcgcctc
     4081 gtccacgcgc acccggcgct tcgcggaggg cacgccgcag agcgcggcgc ggaacgcgac
     4141 gtactccctc acgcgcatct ccgggtacag cgcgagcgtc tcgggcaggt agccgagttc
     4201 gcgcttggcc tcgagcggtt tctccgtgac gtccatgtcc gcgaggagca cgcgccccgc
     4261 cgtcggcggc aggaagcccg tgatcatgcg catcgtggtc gtcttgccgg ccccgttcgg
     4321 gccgaggaac cccagaatct cgcccgtctc gacgcggaag ctcacgtcct cgacggcaga
     4381 cgtcccgtag tacgacttgg aaagtccctg aacttcgagc attgcgttcg gtgtcctccg
     4441 cgaaaggccc ggaccggcgg gggcccccgc ccccgggtgc cggaagcgcc ccggaatata
     4501 gcagccgagg gcttaggatc agacgccagg gaggctcgat gtcccagccc gtcgccaagg
     4561 tcgtcgatct cacggatccc acgctcacgt ccctccgcgg gacctccgtc gaagacgccc
     4621 gcgcgcgcgt cgcccgcggg gacgccgagg gagtcgccgc cctcgacggc tcgtacgcgc
     4681 tcgtcggcgt cgacggaatc gccgtccgcc tcgcgcgctc gatggaccgg ccgctgcgct
     4741 acttcctcgc caaggaggcc gccggccccg ttctcgtcgt cgcggaccgg atcgacgcga
     4801 tcgcgcgctg gctcgcggac cacggccacg cggaccagtt ccacccgagc tacacgcgca
     4861 tggtcccggc ccaccacctc gccaccgtgc gcctcgtcgg ctgcccggat cccgagccgt
     4921 cctacgtgcg gacgttcacc ccgcagcgga acgccctgcc ggccgacctc gacgcgatcg
     4981 ggcgcctcta cgtctcggcg ctcgcggacg agatcgcggg ctggatcgag cgcgtccccg
     5041 cgcaggcgcc gctcggcgtc tgcttctccg gcggtatcga cagcggcgcc gtcttcctcg
     5101 tgacccatca cgtcctccgg aagctcggca tgagcccggc gcgcctcaag gcgttcactc
     5161 tgacgctcgg cggcgggccc gacctcgcgc aggcccgcgc gttcctcgac acgtgtgaca
     5221 tgagcttctt cctcgagccg gtcgaggccg acgccgcgtc gctcgatccc ttcgaggcgg
     5281 tccgcgtgat cgaggactac aagcccctcg acgtcgagtc cgcgacgatg gcgatcgcgc
     5341 tcctgcgcgg tatccgcgcg cgctacccgg agtggacgca cctcctcgac ggcgacggcg
     5401 gggacgagaa cctcaaggac tacccgatcg aggagaaccc cgagctcacg atccggagcg
     5461 tcgtgaacaa cctgatgctc taccaggagg gctggggggt ggggtcgctc aagcactcgc
     5521 agacgtacac cggcggccag agccgctcgt acgcgcgcac gtacgccccc ctccgactct
     5581 tcggcttcac gggcttcagc cccttcacgc ggccgcgcgt cgtcgcggtg gcggagggga
     5641 tcccgttcgc ggacctcacg cagggcagcg tcgagaagct ctacgccctg aagggcgaga
     5701 tcgtcggccg cggcgtgaaa gccgtcacgg gcctgacgat gcccgttttc cccaagcgcc
     5761 gcttccagca cggcgccggc ggcgcggccg tccacgcgca gcgcttcccg tacggtgagg
     5821 cccgctaccg ccgcgccctg atcgaggcct tcgcgggagc cgcgtgagcg ccgttttccc
     5881 agagacgcgc gcggaacgcg agcggttcat ggcggcgcgc cgcccggcga agaacgtcct
     5941 cgacccgcgg cgcccgtatg gcttcttcgt ggaggacgag cccgccgcgc cgggcgtgcc
     6001 ggtgccggtc gcgacgatcc tcctcacgaa ccgcgagtgc acgacccgct gcacgatgtg
     6061 cgacctctgg aagaacacgc tggacggccc gacgccgccg ggcgcgatcc cggagcagat
     6121 ccgctgggcg ctcgagcggc tgcccgcggc gcgccggatc aagctctaca acgccggaag
     6181 cttcttcgac cgggccgccg tcccgccgga ggaccacgcc gcgatcgcgg cgctcgtccg
     6241 cggattcgag cgcgtcgtcg tcgagtgcca cccggcgctc gcgggcgacg cgttgctgcg
     6301 cttccgcgac ctcctcgcgg ggccggagct cgaggtggcc gtcggcctcg agacggcgaa
     6361 cgaggtcgcc ctcgagaaga tcaacaaggg gatgacggtc gaggaattcg aggcgtgcac
     6421 ccgccgcctc gtctcgcgcg gcctcccggt gcgcagcttc gccctcgtag gagttccgtt
     6481 tcttagaaag gaagaatggg cggaggccac gcgcgcgagc atcgacgtct cgttccgggc
     6541 cggagctgaa atcgtctcgc tcatcccgac gcgcctcggc aacgggacgc tcgaagagct
     6601 tcagcggacc ggcgatttca cacctccagc gttgagcgat ctcgaacggg cgatggaaga
     6661 tttcttcgaa ggtgaagagg ggcaggccgg cgcaacgggg atgctgctcg cggacttgtg
     6721 gaatgcgcgc gacttgccgg cgcccgcatg ctgcgcctcc gcccgcatcg agcgcctgcg
     6781 cgccatgaac acctttcaaa gaaatctccc tcttcctcct tctctttctc ctcttccctg
     6841 tcccgtttgc tcgtgaacga agccttcgac gtcgcggtcg tcgggtcggg gttcgccggc
     6901 tcgctcttcg cgctcgtcgc gcggcgcgcg gggcggacgg tggcgctcct cgagaagggc
     6961 gcccacccgc gcttcgcgat cggcgagtcg tcttccccgc tcgcgaacct cctcctcgag
     7021 gagatcgcgg cgcgtttcga gctgcctcgc ctgcttcccc tcacgacgtg ggggacgtgg
     7081 cgccgcacgt acccggagat cacctgcggc ctcaagcgag gcttcacctt ctacgcgcac
     7141 gaggagggcc gccgtttcgc cggcgccccc gaccggagcg accagctcct cgtcgccgcg
     7201 agcccgaacg acgccgtcgc cgacacgcac tggtaccggc ccgacttcga ccagttcctc
     7261 gtgcgcgagg ccgaggccga gggcgcggcc taccgcgacc gctcccgcct cgcagccgtc
     7321 tcgcgcgagg gcgacggctg gcggctcgag ggcgaccggc tcggcgagcc gttctaccgg
     7381 ctggagatgc cggagtcggg ttggatgagc ggagtgcgcg gcgcggtgct ggaggcgatc
     7441 gggcaggtgt atttcgcgac ggcgacgggc atccagatgt gcgaggcgaa tgggcgcgtg
//
LOCUS       DAOMFY010000194         7493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3115_length_7493_cov_8.826701, whole genome shotgun sequence.
ACCESSION   DAOMFY010000194 DAOMFY010000000
VERSION     DAOMFY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3115_length_7493_cov_8.826701"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLB01_13975"
     CDS             <1..843
                     /locus_tag="PLB01_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78457.1"
                     /translation="TVVACVGGGSNAIGLFHAFLGDSDVEIVGVEAGGRGDAPGQHAR
                     TLAQYEKARAAKTRLLALSPRYDADEMSVYESTLAETGARIALTRRATRARLETEIAR
                     AAEALGSPFRGVALELVSDLPVEGEAADLARALAVLMRERKADERRAQRCLAGPHRDD
                     VVLKAEGVPVAARASSGETRTFLLAWTLAEMTLMAESAGNPPVLAFDDFDSEWDPRVL
                     GAFAEALPEDGQIFLSSARPEAVRGLPLPAGALYRMAAGRLAREGILGAGRAATRVAR
                     GGAR"
     gene            840..3353
                     /gene="gyrB"
                     /locus_tag="PLB01_13980"
     CDS             840..3353
                     /gene="gyrB"
                     /locus_tag="PLB01_13980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQQ78458.1"
                     /translation="MSAAGAPGDPVAPGAGVPVDPAAANPNAGDDYGAASIKLLKGLE
                     AVRKRPGMYIGNTDDITGLHHMVTELVDNAIDEAQAGYCDTISVVVHADDSVTVEDNG
                     RGIPVGPHPVHKRETLEIILTDLHSGGKFDDNAYKVSGGLHGVGLSVVNALAGDLLVE
                     VKREGKVWQQRYGKGVPLEPIREVGKTAKTGTKITFKADPEIFSVTSYNFDALSQRLR
                     ELAFLNRGVTIEAVDERDPKGEKKHVFKYEGGIKSFVEHLNKNKAPLHEAVITFADVK
                     NPTGDPAEDAKHERLDVALQWNEGYAEQIFCFTNTISNKDGGTHLEGLKAALTRAIQR
                     YAESTGLTKNLKDTTLAGDDCREGLTAVVSLKIRDPKFSSQTKEKLVSQEAKTWVQTV
                     TYERLSSFFEENPKVARRIVEKIVDAARAREAARKARELVRRKGALDGAGLPGKLADC
                     QEGDPALSEIFIVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKMLAFAEIR
                     SLILALGAGIGEDFDAGKIRYHKIILMTDADVDGSHIRTLLLTFFFRQMRAAIERGYL
                     YIAQPPLFRVAEGKKETFLKDEKEKARFLLARLGEDRTLVSETTEKTASGAALTALVA
                     EMQEWRGLVARLAMRGFPEALLRTLVNGGLTDPAAFADRARLERAAQELAAVVEAFEI
                     RADEEHGGHAIHVTQAVNGVVKTGVVDAAFLAGYEMRKVADLAAKIDGFLDGPYRLRA
                     NGSGAELVLATLPEAVDAVLASAEKGLTINRYKGLGEMNPETLWETTMNPASRRLLQV
                     RVEDAVEADEIFTILMGDAVEPRREFIEANALNVSNLDI"
     gene            3355..5973
                     /gene="gyrA"
                     /locus_tag="PLB01_13985"
     CDS             3355..5973
                     /gene="gyrA"
                     /locus_tag="PLB01_13985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQQ78459.1"
                     /translation="MADTPEKPPVIDPTEKIPVSIEEEIRRSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLFGMWEQGNRAGRPYKKSARIVGDVMGKYHPHGDSAIYDTIVRMAQ
                     PFSLRETLVDGQGNFGSVDGDAAAAMRYTEIRLARLAEDLIGDDIDKETVDWTLNYDG
                     SLQEPVVLPCAFPNLLVNGSEGIAVGMATKIPPHNLAEACDAAVLLLDEPKTDLAALL
                     KVLPGPDFPTGGIILGRQGIVDAYRTGRGILQVRGKAGIEKSRKGDRESIVITEIPFQ
                     VNKARLIELIAHLVNEKRLEGISAIRDESDREGMRVVVDVKRDAVAQVVLNQLLALTP
                     LQSSFGIIFLAIVDGQPRVLPLKDLFRHFLDHRRSVVVRRTQFDLRKAEERAHVLLGL
                     AVALASLDRVIAIIRASKDGAEAKERLCAEVVVEKAALEKFLGVDSLEISASARTGDG
                     LLRLDAVQAQAILDMRLQRLTGLEREKILAEYREVLALIADLKDVLAKPARVAAIIRA
                     ELLAVKERFGNPRRTEIQAEAGTLEMEDLIAEEEVVLTISRGGYAKRTPLSVYRAQRR
                     GGKGRTGAATAAEDVVEHLFVGSTHDYLLAFTNKGRVHWVKVYDLPSLAPATRGKALV
                     NFLALEEGERLAAMATTRTFPDDRFLIFATRNGTVKKTLLSAYGNPREKGIIAIKLED
                     GDELLSAQITDGTRKVFLGTRNGMGIKFEETDVRAMGRDTTGVKGIELREGDVLVEMD
                     LVEDSSTLLAVTEKGYGKRTDIAEYRQQGRGGTGVINVKVTEKNGPVVGVSSVTDTDH
                     LLLITEQGILIRIAVSDVRETGRATMGVKLIDLAEGDRVVAVAKLAGEDVPNGEAEPP
                     AESPTAGEGPQSAKTG"
     gene            5986..6639
                     /locus_tag="PLB01_13990"
     CDS             5986..6639
                     /locus_tag="PLB01_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HQQ78460.1"
                     /translation="MRFFLDTADADEARRVREWGLLDGILLRPDAAGAPGRDTRRLLA
                     ELAQVGDGPVVATLAAGDPKAMYKEARELHKHGKNVVLRVPMTREGLRVVRLLGDEQI
                     ATDVGLVYTASQALLAARAGSAYVSPSVGELDDAGQIGMDVVESIVRVYDNYGLETQI
                     CVQGIQNPIHVLDAAALGADVATLPFAVLDKLFDHPLTARGVALLRAPAGDGPRRIH"
     gene            6636..6917
                     /locus_tag="PLB01_13995"
     CDS             6636..6917
                     /locus_tag="PLB01_13995"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HQQ78461.1"
                     /translation="MISRERAVDLSHRMVERLGKTPGVAVVAEREFLRNHILQALLAW
                     DKENDRITEAVKAKIAARSRRVVEGTREWDLLVAEEMERAYAALLSRGE"
     gene            7003..>7493
                     /locus_tag="PLB01_14000"
     CDS             7003..>7493
                     /locus_tag="PLB01_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQQ78462.1"
                     /translation="MATTASERATPATRVPLYRTIADAVIRDIRRGKHLEGERLPASR
                     DLARALDVNRATVHQALQILKREGWIETNKGGGTRVARRSSQAPVLGPAPAWDRIASS
                     LLLRIVSEPPAAEPVASPYDLSRLAPDERDFPAAAFAALLAEVARDGALLAYGSPFGY
                     RPLR"
BASE COUNT         1209 a         2708 c         2605 g          971 t
ORIGIN      
        1 acggtggtcg cctgcgtggg tggcggcagc aacgccatcg gcctcttcca cgcgttcctc
       61 ggcgacagcg acgtggagat cgtgggtgta gaagcgggag gccgcggaga cgcgcccggc
      121 cagcacgcgc gcacgctcgc gcagtacgag aaggcgcgcg cggccaagac gcggctcctc
      181 gcgctctcgc cgcgttacga cgccgacgag atgtccgtct acgagtccac cctcgccgag
      241 accggcgcgc ggatcgcctt gacccgccgc gcgacgcgcg cgcgcctcga gaccgagatc
      301 gcgcgcgccg ccgaagccct cgggtccccg ttccggggcg tcgcgctcga gctcgtgagc
      361 gacctcccgg tggagggcga ggcggcggac ctcgcgaggg ccctggccgt gctcatgcgg
      421 gagaggaagg ccgacgagcg gcgggcccag cgctgccttg ccgggccgca tcgcgacgac
      481 gtcgtgctga aggccgaggg cgtcccggtc gcggcgcgcg cctcctccgg cgagacgcgg
      541 acgtttcttc tcgcgtggac gctcgccgag atgacgctca tggccgaatc ggcgggcaac
      601 ccgccggtcc tcgcgttcga cgacttcgac tcggagtggg acccgcgcgt cctcggcgcg
      661 tttgccgagg cgctcccgga ggacgggcag atcttcctct cgtccgcgcg gccggaggcc
      721 gtgcggggcc tcccgctgcc cgcgggcgcc ctttaccgca tggcggccgg ccgcctcgcg
      781 cgcgagggaa tcctcggagc gggacgggcc gcgacgcgcg tcgcccgtgg aggagcgcga
      841 tgagcgccgc gggagcgccg ggagatcccg tggcgcccgg ggccggcgtg ccggtggacc
      901 cggccgcggc gaacccgaac gcgggagacg actacggcgc cgcgtcgatc aagctcctca
      961 aggggctcga ggccgtgcgc aagcgccccg ggatgtacat cgggaacacc gacgacatca
     1021 cgggcctgca ccacatggtc accgagctcg tcgacaacgc gatcgacgag gcgcaggcgg
     1081 gctactgcga cacgatcagc gtcgtcgtcc acgccgacga ctccgtcacg gtcgaggaca
     1141 acggccgggg catcccggtc ggtccgcacc cggtccacaa gcgcgagacg ctcgagatca
     1201 tcctcacgga cctccactcg ggcggcaagt tcgacgacaa cgcgtacaag gtctccggcg
     1261 gcctccacgg cgtcggcctg tccgtcgtga acgcgctcgc cggcgacctc ctcgtcgagg
     1321 tcaagcgcga ggggaaggtc tggcagcagc gctacgggaa gggcgtcccg ctcgagccca
     1381 tccgcgaggt cggcaagacc gcgaagacgg gcacgaagat cacgttcaag gccgaccccg
     1441 agatcttctc cgtcacgagc tacaacttcg acgccctctc gcagcgcctg cgcgagctcg
     1501 cgttcctgaa ccggggcgtc acgatcgagg ccgtcgacga gcgcgacccc aagggcgaaa
     1561 agaagcacgt cttcaagtac gaaggcggga tcaagtcctt cgtcgaacac ctcaacaaga
     1621 acaaggcgcc gctgcacgag gccgtcatca cgttcgcgga cgtgaagaac ccgaccggcg
     1681 acccggccga ggacgccaag cacgagcggc tggacgtcgc gctccagtgg aacgagggct
     1741 acgccgagca gatcttctgc ttcacgaaca cgatctcgaa caaggacggc ggcacgcacc
     1801 tcgagggcct caaggccgcg ctcacgcgcg cgatccagcg ttacgccgag agcaccggcc
     1861 tcacgaagaa cctcaaggac acgacgctcg ccggcgacga ctgccgcgag ggcctcacgg
     1921 ccgtcgtctc gctcaagatc cgggacccga agttctcgtc gcagacgaag gaaaagctcg
     1981 tctcgcagga ggcgaagacg tgggtccaga ccgtcacgta cgagcgcctc tcgagcttct
     2041 tcgaggagaa cccgaaggtc gcgcggcgga tcgtcgaaaa gatcgtggac gcggcgcggg
     2101 cgcgcgaggc ggcccgcaag gcgcgcgagc tcgtccggcg caagggcgcg ctggacggcg
     2161 cgggcctccc cggcaagctc gcggactgcc aggaaggcga tccggcgctc tcggagatct
     2221 tcatcgtcga gggcgactcg gccggcggct cggccaagca gggccgcgac cgccggacgc
     2281 aggcgatcct ccccctcaag ggcaagattc tgaacgtcga gaaggcgcgc ttcgacaaga
     2341 tgctcgcctt cgccgagatc cgctcgctca tcctcgcgct cggcgcgggg atcggcgagg
     2401 acttcgacgc cggcaagatc cgctaccaca agatcatcct catgacggac gccgacgtgg
     2461 acgggagcca catccggacg ctcctcctga cgttcttctt ccgccagatg cgcgccgcga
     2521 tcgagcgcgg gtacctctac atcgcgcagc ccccgctctt ccgcgtcgcg gagggcaaga
     2581 aggagacgtt cctcaaggac gagaaggaga aggcgcgctt cctcctcgcg cgcctcggcg
     2641 aggaccgcac gctcgtctcc gagacgacgg agaagaccgc ctccggcgcc gccctgaccg
     2701 cgctcgtcgc cgagatgcag gagtggcgcg gcctcgtcgc gcgtctggcc atgcgcggct
     2761 tccccgaggc gctgctgagg acgctcgtga acggcggact cacggacccg gcggcgttcg
     2821 ccgaccgcgc gcgcctcgag cgcgccgcgc aggagctcgc ggccgtcgtg gaggccttcg
     2881 agatccgcgc ggacgaggag cacggcggcc acgcgatcca cgtcacgcag gccgtcaacg
     2941 gcgtcgtgaa gacgggcgtc gtcgacgccg cgttcctcgc gggctacgag atgcgcaagg
     3001 tggccgacct cgccgcgaag atcgacggct tcctcgacgg cccgtaccgc ctgcgggcga
     3061 acggttccgg cgccgagctc gtgctcgcga cgcttccgga ggccgtggat gcggtcctcg
     3121 cgagcgcgga gaagggcctg acgatcaacc ggtacaaggg cctcggcgaa atgaaccccg
     3181 aaacgctctg ggagacgacg atgaaccccg cgtcgcgccg cctcctccag gtgcgcgtcg
     3241 aagacgccgt cgaggcggac gagatcttca cgatcctgat gggcgacgcg gtcgagccgc
     3301 gccgcgaatt catcgaggcg aacgcattga acgtctcgaa cctggacatc tgagttggcc
     3361 gacacccccg aaaagccccc cgtgatcgac ccgacggaga agatccccgt ctccatcgag
     3421 gaggagatcc ggcggagcta cctcgactac gcgatgtccg tcatcgtcgg gcgcgccctg
     3481 cccgacgtgc gcgacggcct gaagcccgtg caccgccggg tcctcttcgg aatgtgggag
     3541 cagggcaacc gcgcgggccg cccgtacaag aagtccgccc gcatcgtcgg cgacgtcatg
     3601 ggcaagtacc acccgcacgg cgactcggcg atctacgaca cgatcgtccg gatggcgcag
     3661 ccgttctccc tgcgcgagac gctcgtggac ggtcagggca acttcggctc cgtggacggc
     3721 gacgcggccg cggcgatgcg ctacacggag atccgcctcg cgcgcctcgc cgaggacctc
     3781 atcggcgacg acatcgacaa ggagaccgtc gactggacgc tgaactacga cggctcgctc
     3841 caggagccgg tcgtcctgcc gtgcgcgttc ccgaaccttc tcgtgaacgg gtcggaaggc
     3901 atcgcggtcg gcatggcgac gaagatcccg ccgcacaacc tcgccgaggc gtgcgacgcg
     3961 gccgtcctcc tcctcgacga gccgaagacg gacctcgccg cgctcctcaa ggtcctgccc
     4021 ggcccggact tcccgaccgg cggcatcatc ctcggccgcc agggcatcgt ggacgcctac
     4081 cgcacgggcc gcgggatcct gcaggtgcgc ggcaaggccg ggatcgagaa gtcccggaaa
     4141 ggcgaccgcg agtcgatcgt catcacggag atcccgttcc aggtcaacaa ggcgcgtctc
     4201 atcgagctca tcgcgcacct cgtgaacgag aagcggctcg agggaatctc ggcgatccgg
     4261 gacgaatcgg accgagaggg catgcgggtc gtcgtcgacg tcaagcgcga cgccgtggcg
     4321 caggtcgtcc tgaaccagct cctcgcgctc acgccgctcc agtcgtcgtt cgggatcatc
     4381 ttcctcgcga tcgtggacgg gcagccgcgc gtcctcccgc tgaaggacct cttccgccac
     4441 ttcctcgacc atcggcgctc cgtcgtcgtg cgccgcacgc agttcgacct ccggaaggcc
     4501 gaggagcgcg cgcacgtcct cctcggcctc gccgtcgcgc tcgcgagcct cgaccgcgtc
     4561 atcgcgatca tccgcgcgag caaggacggc gcggaggcga aggagcggct gtgcgcggag
     4621 gtcgtcgtcg agaaggcggc cctcgagaag ttcctcggcg tcgactcgct cgagatctcc
     4681 gcctccgcgc gcaccggcga cggcctcctg cgcctcgacg cggtccaggc gcaggcgatc
     4741 ctcgacatgc gcctccagcg cctcacgggc ctcgagcgcg agaagatcct cgccgagtac
     4801 cgcgaggtcc tcgcgctgat cgccgacctg aaggacgtcc tcgcgaagcc cgcccgcgtc
     4861 gcggcgatca tccgcgccga gctcctcgcc gtgaaggagc ggttcgggaa cccgcgccgc
     4921 acggagatcc aggcggaggc cggcacgctc gagatggagg acctcatcgc cgaggaggag
     4981 gtcgttctca cgatttcgcg cggcggctac gcgaaacgga cgcccctgtc cgtgtaccgc
     5041 gcgcagcgcc gcggcggcaa gggccgcacg ggcgccgcga cggccgccga ggacgtcgtc
     5101 gaacacctct tcgtcggctc gacgcacgat tacctcctcg ccttcacgaa caagggccgc
     5161 gtccactggg tcaaggtcta cgacctcccg tcgctcgcgc ccgcgacgcg cggcaaggcc
     5221 ctcgtgaact tcctcgccct cgaggagggg gagcgcctcg cggcgatggc gacgacgcgc
     5281 acgtttcctg acgaccgctt cctgatcttc gcgacgagga acggcaccgt gaagaagacg
     5341 ctcctctcgg cgtacggcaa cccgcgcgag aaggggatca tcgcgatcaa gctcgaggac
     5401 ggcgacgagc tcctctcggc gcagatcacg gacggcacgc ggaaggtctt cctcgggacg
     5461 cgaaacggga tggggatcaa gttcgaggag acggacgtcc gcgcgatggg ccgcgacacg
     5521 acgggcgtca aggggatcga gctccgcgaa ggggacgtcc tcgtcgagat ggacctcgtc
     5581 gaggactcgt cgacgctcct cgcggtcacg gagaagggct acggcaagcg gacggacatc
     5641 gcggagtacc gccagcaggg ccgcggcggc accggcgtca tcaacgtgaa ggtcaccgag
     5701 aagaacggcc ccgtcgtcgg cgtctcgtcc gtcacggaca cggaccacct cctcctcatc
     5761 acggagcagg ggatcctgat ccggatcgcg gtctcggacg tgcgcgagac gggtcgcgcg
     5821 acgatgggcg tcaagctcat cgacctcgcg gagggagacc gcgtcgtcgc ggtcgcgaag
     5881 ctcgcgggcg aagacgtccc gaacggcgag gccgagcctc cggccgagag ccccacggcc
     5941 ggcgaaggcc cgcaaagcgc caagacgggc tagactccgc cccccatgcg cttcttcctc
     6001 gacactgcgg acgcggacga ggcccgccgc gtccgcgaat ggggcctcct cgacgggatc
     6061 ctcctccggc cggacgccgc cggcgccccc gggcgggaca cgcgccggct cctcgccgag
     6121 ctcgcgcagg tcggcgacgg tcccgtcgtc gcgacgctcg cggcggggga cccgaaggcg
     6181 atgtacaagg aagcgcgcga gctccacaag cacggcaaga acgtcgtcct gcgcgtgccg
     6241 atgacgcgcg agggcctgcg cgtcgtgcgc ctcctcggcg acgagcagat cgcgacggac
     6301 gtcggcctcg tctacacggc ctcacaggcg ctcctcgcag cccgcgccgg cagcgcgtac
     6361 gtgagcccgt ccgtcggcga actggacgac gcgggccaga tcgggatgga cgtcgtcgag
     6421 tcgatcgtgc gcgtgtacga caactacggc ctcgagacgc agatctgcgt gcaggggatc
     6481 cagaacccca tccacgtcct cgacgcggcc gcgctcgggg ccgacgtcgc gacgctgccg
     6541 ttcgccgtcc tcgacaagct cttcgaccac ccgctcaccg ccaggggcgt cgcgctcctg
     6601 cgcgccccgg cgggcgacgg gccgaggaga attcattgat ctcccgcgag cgggccgtgg
     6661 acctttcgca ccggatggtc gagcgtctcg gcaagacgcc cggcgtcgcg gtcgtggccg
     6721 agcgcgagtt cctccggaac cacatcctcc aggccctcct cgcgtgggac aaggagaacg
     6781 accggatcac ggaggccgtg aaggcgaaga tcgccgccag gtcgcggcgc gtcgtcgagg
     6841 gcacgcgcga gtgggacctc ctcgtcgccg aggagatgga gcgcgcatac gcggcgctcc
     6901 tgtcgcgcgg cgagtagccc tggccgggcg gcccccgccc ttggttggtc tgtgcgggcg
     6961 ccgggtttga cgttggtcta ggccaagctc taacgtgtct gcatggcgac gacggcctcc
     7021 gagcgcgcga ccccggcgac gcgcgtcccg ctctaccgga cgatcgcgga tgcggtgatc
     7081 cgcgacatcc gcagaggcaa gcacctcgag ggcgagcggc tgccggcctc ccgcgacctc
     7141 gcgcgggccc tggacgtgaa ccgggcgacc gtgcaccagg cgctccagat cctcaagcgc
     7201 gagggctgga tcgagacgaa caagggcggc gggacgcgcg tcgcgcgccg ctccagccag
     7261 gccccggtcc tcgggcccgc acccgcgtgg gaccgcatcg cgtcgtcgct cctcctgcgc
     7321 atcgtctccg agccgccggc ggccgagccc gtcgcgtcgc cgtacgacct gtcgcgcctc
     7381 gcgcccgacg agcgcgactt cccggccgcc gcgttcgccg cgctcctcgc cgaggtggcg
     7441 cgggacggcg cgctcctcgc gtacggctcg ccgttcggct accggccgct gcg
//
LOCUS       DAOMFY010000195         7314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3238_length_7314_cov_7.298801, whole genome shotgun sequence.
ACCESSION   DAOMFY010000195 DAOMFY010000000
VERSION     DAOMFY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_7314_cov_7.298801"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1933
                     /locus_tag="PLB01_14005"
     CDS             38..1933
                     /locus_tag="PLB01_14005"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQQ78463.1"
                     /translation="MTLPQDGSLLRIFVGESDRRGGKLLYEWLVLRALAARRGAPTIG
                     RAFLVATPSEGTPLASPARWDEMAAWVANLAEALPAGALAFGLDFAVEALVWIARRAG
                     GALPGLAAMDPAGEFLAALNARPADRVTYGAAVARFTPGGSLAKRLLDAGAEAIFRAP
                     NDLVVPTEGGLSLGRRRIDETRVVRFDGGVHHLNLFAQKEAREGLKIFLEGEEGTGTA
                     HAHEKRRSGPRGRGRSDGRLPKPYSPSKKSSSSPLPSSARSAQRFDILLVDGTPPRLV
                     ASWAGARVVEPFETRGGVAGERMRKIIALHERVLRALDGRGAGPLPKAAELHAYGAVL
                     FETLFPGEVRRLWDAARGASARPLELAFTSALDWIADKPWELAWDPSRRRFLSDDAVL
                     VRNVFGAIPPEAPAARQGPLRVLVAVAQPRDATPVSARLETAAIRSAFAPLVKSGHAV
                     LDVLPAATPSGLHRKLGAARVDVLHFVGHGAWDEREGTGALLLVDGRGRGTWLDADRL
                     RRLLCGRGLKLVVLNACETGRGGRNDFLRGVAPALLAGGIPAVVANQYKVLDTSATAF
                     ARHLVWALARGHALGEAAREARVAVAVARSAEPMDWAVPVLYARDTSFVLCRPARRKA
                     AEGSRHS"
     gene            1945..3969
                     /locus_tag="PLB01_14010"
     CDS             1945..3969
                     /locus_tag="PLB01_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HQQ78464.1"
                     /translation="MRAPLAASAAFVLLAPAAPARAAVAVAVDPNADRHPVSPLIYGM
                     NYGTAAQMSRLKVPVRRWGGNSTTRYNWENDTHNSASDWFFFNYAGSANPANLPNGSD
                     ADVFVDEIRAAGGEALVTVPLIGWTPIDRTRRWGFSVAKYGAQQQTECSATGNASWCN
                     PDAGNGVKPNSANVTGNDPADTSKPIDATFVTRWMAHLAGRTGTAAAGGVRLWALDNE
                     PMLWNSTHRDVHPAGTTYDELWAKTAAIGAAMKAQDPGAKLLGPALWGWCAYFYSGAD
                     GCGPGPDRAAHGGQDFLPWYLDQAKAWEVSNGKRLLDTLDVHYYPQANGVALTNDESS
                     ATAALRLRTLKSLYDPTYLDESWIGTDVGQAVYLIPRMTAWIAAHYPGTKLAISEYNW
                     GSDAGISGALAQAEALAIFGREGVDLATRWVAPADGSLTEDAFLLYRNYDGAGAQVAG
                     DSVRATSANVDLVGAYAQRGGAGGNTLYLLLFNKDVSAQSVPATVAGGLSGSASLFRF
                     TGSSRLAAAGSAAPSGGTLTLTLPARSATLAVVALPVTTPPPAATSFHAATPCRFLDT
                     RAAAGPALAANVRREFVLTGTCGIPATAAAVSANVTVTAPSSAGSLVAFPADLTSAPG
                     TNTLSFGAGQTRANNAMLLLARDASGRCAFTPAMPSGAVHLVVDVNGWWE"
     gene            4085..4324
                     /locus_tag="PLB01_14015"
     CDS             4085..4324
                     /locus_tag="PLB01_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78465.1"
                     /translation="MPQWAAVLRARGYAFTTLARAPEDPAYATPDSFVGKPGFSWLHR
                     WLFGKADATRLKEEPDPPPFVMDAYAAIEKRRAAR"
     gene            complement(4349..5230)
                     /locus_tag="PLB01_14020"
     CDS             complement(4349..5230)
                     /locus_tag="PLB01_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78466.1"
                     /translation="MKSHPQPVRVLAAAFVAALVFSVAAPAEAGSNRSGSAAAARYES
                     LVAAGAKDGPALYRAGMDLLNAGRYDLAEKAFLASAAAGNRVAASTYNAACALARAGK
                     KPEALDALQKAIEQGYTDARHMERDDDLESIQDEKRFTELVRLAEDLSLDAGVEGFMG
                     HFLPNTARAAWRGEIPRYEKVAKAHPTLGIAWYNLGYAQLMGDRPEAAAESFGKALGL
                     GYRRPTTLYNLACAEARSGDTNRAFDHLFAAIDAGFDGADHMRSDADLDSIRKDPRFR
                     QAVAKARARSKDADEED"
     gene            complement(5270..5704)
                     /locus_tag="PLB01_14025"
     CDS             complement(5270..5704)
                     /locus_tag="PLB01_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78467.1"
                     /translation="MSKETPDHNDAELAFRAYELRREAVMRESRNAINRDFWPKTYED
                     VLAVTKFDHPLNAPWRQTTTYWEMVYGTVKHGIVNADYFMESAGEGIFLFARVAPYLD
                     ELRRDVSPVAFQNAEWVAKNTGTGQRMFAMFTARVQKLLAAR"
     gene            complement(5745..6059)
                     /locus_tag="PLB01_14030"
     CDS             complement(5745..6059)
                     /locus_tag="PLB01_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78468.1"
                     /translation="MAETPAPAPAPAPEKCFLCNFPASISDEHTLFASSLVGFSVDCE
                     ECGSYAITPDAIHRLSVRPVSKPGVRFEIFRLRSNGQTPRPTVDLKMVEHFCTGYTPM
                     RG"
     gene            6102..>7314
                     /locus_tag="PLB01_14035"
     CDS             6102..>7314
                     /locus_tag="PLB01_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ78469.1"
                     /translation="MRRPDPGLVFVLPLLAFALTRPSRARGADPGERPPYPPTRTTQA
                     SEVLHGVTVMDPYRWLEDEKSPEVQAWMKAQDDFTRRTLASLPERAAIAARLKELLYV
                     DSLGAPIHRGTRHFFSRRHADREKSIVYWKEGAGGAENVLLDPNGWSADGSAGLGAWS
                     VSWDGARVAYGKKVNNSDEATLYVMDVATGKVSDVDVIEGAKYASASWTPDGAAFYYT
                     KLPVDPKIPASERPGWADVRLHRIGTDPKTDTVVKAKLGDPTAFQNVHITRDGRFLFL
                     VVAHGWTSTEIWFRDLETDPAATAWTPLATGARAHFDVETFGRTLFVSTDDGAPKGRV
                     FAVDPAKPARAAWKEIVPERKDAALQSFSVVGGKLALDYLRSAASLLEVRNLDGTLLR
                     DVPLPGIGSASN"
BASE COUNT         1003 a         2694 c         2574 g         1043 t
ORIGIN      
        1 cggccgccac ctcgcccagc tggtctggag gtgactcttg accctccccc aggacggctc
       61 cctcctccgg atcttcgtcg gggagagcga ccggaggggc ggcaagctcc tctacgagtg
      121 gctcgtcctg cgcgccctcg ccgcccgcag gggcgcgccg acgatcggcc gcgccttcct
      181 cgtcgcgacg ccgtccgagg gaacgccgct cgcgtcgccg gcccgatggg acgagatggc
      241 cgcctgggtc gcgaacctcg ccgaggcgct gcccgccgga gctctcgcct tcggcctgga
      301 tttcgcggtg gaggcgctgg tctggatcgc gcggcgcgca ggcggcgcgc tgcccggcct
      361 cgccgcgatg gatccggcgg gtgagttcct cgcggccctc aacgccaggc ccgcggaccg
      421 ggtcacgtac ggcgccgccg tcgcgcgctt cacgccgggc gggagcctcg cgaagcgcct
      481 cctcgacgcg ggcgccgagg cgatcttccg cgcgccgaac gacctcgtcg tcccgacgga
      541 aggcggcctc tccctcggcc gccggcgcat cgacgagaca cgggtcgtgc gattcgacgg
      601 cggcgtccac cacttgaatc tctttgctca gaaagaggcg agagagggat tgaagatttt
      661 cttagaaggt gaagagggga ctggcaccgc ccatgcgcac gaaaaacgcc ggagcgggcc
      721 gcggggacgc ggccgaagcg acggccgcct ccccaagccc tattcacctt caaagaaatc
      781 ttcctcttct cctcttccct cttccgcgcg cagcgcgcag agattcgaca tcctcctcgt
      841 cgacggcacg ccgccgcgcc tcgtcgcgtc gtgggcgggt gcgcgcgtcg tcgagccctt
      901 cgagacgcgg ggcggcgtcg cgggcgagcg gatgcggaag atcatcgcgc tccacgagcg
      961 cgtgctgcgt gcactcgacg ggcgcggcgc ggggccgctg ccgaaggccg ccgagctcca
     1021 cgcgtacggc gcggtcctct tcgagacgct gttcccgggc gaggtccgcc gcctctggga
     1081 cgccgcgcgg ggcgcgtccg cgcgcccgct cgagctcgcg ttcacgtccg cactcgactg
     1141 gatcgccgac aagccgtggg agctcgcgtg ggacccttcg cgccggcgct tcctctcgga
     1201 cgacgccgtc ctcgtacgga acgtcttcgg cgccatcccg ccggaggcgc ccgcggcgcg
     1261 gcagggcccg ctgcgcgtcc tcgtcgccgt cgctcagccg cgcgacgcga cgccggtctc
     1321 ggcgcggctc gagacggccg caatccgctc ggcgttcgcg ccgctcgtga aatccggcca
     1381 cgccgtcctc gacgtccttc cggcggcgac gccgtcgggc ctgcaccgga agctcggggc
     1441 cgcgcgcgtc gacgtcctcc acttcgtggg ccacggcgcg tgggacgaga gggaaggcac
     1501 gggcgcgctc ctcctcgtgg acggccgcgg acgcgggacc tggctcgacg cggaccggct
     1561 gcggcgcctc ctctgcgggc gcggcctcaa gctcgtggtc ctgaacgcgt gcgagacggg
     1621 ccgcggcgga cggaacgact tcctccgcgg cgtcgcgccc gcgctcctcg cgggcgggat
     1681 cccggctgtc gtcgcgaacc agtacaaggt cctcgacacc tccgcgacgg cgttcgcccg
     1741 ccatctcgtg tgggcgctcg cgcgcggcca cgctctcggc gaggcggcgc gggaggcgcg
     1801 cgtcgccgtg gccgtcgcgc gctccgccga gccgatggac tgggcggtgc ccgtcctcta
     1861 cgcgcgcgac acgtcgttcg tcctgtgccg gccggcgcgc cgcaaggcag ccgaggggtc
     1921 gagacactcc taggcatgcg gggaatgcgt gcgcccctcg ccgcgagcgc ggccttcgtt
     1981 ctacttgcgc ctgccgcgcc ggcccgggcg gcggtcgccg tggccgtcga cccgaacgcc
     2041 gatcgccacc ccgtcagccc cctgatctac ggcatgaact acgggacggc ggcgcagatg
     2101 tcgcggctga aagtccccgt gcgccgctgg ggcgggaact cgacgacgcg ctacaactgg
     2161 gagaacgaca cgcacaacag cgcgagcgac tggttcttct tcaattacgc aggcagcgcg
     2221 aaccccgcga acctcccgaa cggctcggac gcggacgtct tcgtggacga gatccgggcc
     2281 gcgggcggcg aggcgctcgt cacggtcccg ctcatcggct ggacgcccat cgaccggacg
     2341 cgtcgctggg gtttttccgt cgcgaagtac ggcgcgcagc agcagacgga gtgctccgcg
     2401 accggaaacg cctcctggtg caaccccgat gccggcaacg gcgtgaagcc gaacagcgcg
     2461 aacgtcacgg gcaacgatcc ggcggacacg tcgaagccga tcgacgccac gttcgtgacg
     2521 cgctggatgg cgcacctcgc aggccgcacc gggaccgccg ccgccggcgg ggtgcgcctc
     2581 tgggcgctgg acaacgagcc catgctctgg aactcgacgc accgcgacgt ccacccggcc
     2641 ggaacgacgt acgacgaact ctgggcgaag acggcggcga tcggcgccgc gatgaaggcg
     2701 caggatcccg gcgcgaagct cctcggcccc gcgctctggg gctggtgcgc gtacttctac
     2761 tccggcgcgg acggctgcgg cccgggtccc gatcgcgcgg cccacggggg tcaggacttc
     2821 ctcccctggt acctcgacca ggccaaggcg tgggaggtct cgaacggaaa gcgcctcctc
     2881 gacaccctcg acgtccacta ctacccgcag gcgaacggcg tcgcgctcac gaacgacgag
     2941 tcctccgcga cggcggcgct ccgcctgcgc acgctcaaga gcctgtacga cccgacgtac
     3001 ctcgacgagt cctggatcgg cacggacgtc ggccaggccg tgtatctgat cccgcggatg
     3061 acggcgtgga tcgcggcgca ctaccccggc acgaagctcg cgatcagcga gtacaactgg
     3121 ggctcggacg ccgggatcag cggggcgctc gcccaggccg aggcgctcgc gatcttcgga
     3181 cgggagggcg tggacctcgc gacgcgctgg gtcgcgccgg ccgacggctc gctcacggag
     3241 gacgcgttcc ttctctaccg gaactacgac ggcgcgggag cgcaggtggc gggcgactcg
     3301 gtgcgcgcga cgagcgcgaa cgtggacctc gtcggcgcgt acgcccagcg cgggggcgcg
     3361 ggcgggaaca ccctctacct gctcctcttc aacaaggacg tctccgcgca gagcgtcccg
     3421 gccacggtcg ccggcggtct ctcgggctcc gcgtcccttt tccgattcac gggctcgtcg
     3481 cgcctcgcgg cggccggcag cgcggcgccg tcgggcggga ctctcacgct gacgcttccc
     3541 gcgcgctcgg cgacgctcgc ggtcgtcgcc ctccccgtga cgacgccgcc tccggcggcc
     3601 acgtcgttcc acgccgcgac gccctgccgt ttcctcgaca cgcgcgccgc ggccggcccc
     3661 gcgctcgcgg cgaacgtccg ccgcgagttc gtcctgaccg gcacctgcgg gatcccggcg
     3721 acggccgcgg ccgtgtccgc gaacgtcacg gtcacggcgc cgtcctccgc gggcagcctc
     3781 gtggcctttc ccgccgatct cacgtcggcg ccgggcacga acacgctgag cttcggcgcc
     3841 ggccagacgc gcgcgaacaa cgcgatgctc ctcctcgcgc gcgacgcctc cggccgctgt
     3901 gcgttcacgc ccgcgatgcc gtccggcgcc gtgcacctcg tcgtcgacgt caacgggtgg
     3961 tgggagtaga gccgtccggg tttaccctca gggcgggacg cgggcgccga gctcgggaat
     4021 cacacgttct cgcccgatcc gccacgtcct cctcgtccac gcgaaccggc tcaacgcgga
     4081 caccgtgccg cagtgggccg ccgttctccg cgcgcggggc tacgcgttca cgacgctcgc
     4141 gcgcgccccc gaggacccgg cgtacgccac gcccgactcc ttcgtgggca agcccggctt
     4201 ctcgtggctc caccgctggc tcttcggaaa ggcggacgcc acgcgcctga aggaggagcc
     4261 ggacccgccg cccttcgtca tggatgcgta cgcggcgatc gagaagaggc gcgccgcccg
     4321 ctgaaccggg cccggtgcgc cggcgaggtc agtcctcttc gtcggcgtcc ttcgaccgcg
     4381 cacgcgcttt cgcgacggcc tgcctgaaac gcgggtcttt ccggatcgaa tcgaggtccg
     4441 cgtcgctgcg catgtggtcc gcgccgtcga agccggcgtc gattgcggcg aagaggtggt
     4501 cgaaagccct gttcgtgtcg ccggagcgcg cttcggcgca cgcgaggttg tagagcgtcg
     4561 tcggcctgcg gtacccgagg ccgagcgcct tgccgaacga ttcggcagcc gcctcggggc
     4621 ggtcccccat gagctgcgcg tagccgaggt tgtaccaggc gatcccgagc gtcgggtggg
     4681 ccttcgcgac cttctcgtag cgcggaatct cgccccgcca cgccgcgcgg gccgtgttcg
     4741 ggaggaagtg gcccatgaag ccctcgaccc cggcgtcgag agacaggtcc tccgcgagtc
     4801 tgacgagctc ggtgaagcgc ttctcgtcct ggatgctctc gaggtcgtcg tcccgctcca
     4861 tgtgccgcgc gtcggtgtag ccctgctcga tcgccttctg gagcgcgtcg agcgcctcgg
     4921 gcttcttccc ggcgcgcgcg agcgcgcacg cggcgttgta cgtcgaggcc gcgacgcggt
     4981 tcccggccgc cgcggaggcg aggaacgcct tctcggccag gtcgtaccgg ccggcgttca
     5041 ggaggtccat gccggcccgg tacagcgccg ggccgtcctt ggcgccggct gcgacgaggc
     5101 tctcgtagcg agcggccgcg gccgacccgg agcggttcga tcccgcttct gcgggggcgg
     5161 ccacgctgaa gacgagggcg gcgacgaagg cggcggccag gaccctgacg ggctgcggat
     5221 ggctcttcat gggaagagat acgcaccggg cgggaaaggg ttccggacgc tacctcgccg
     5281 cgaggagctt ctggacccgg gccgtgaaca tcgcgaacat cctctggccc gtccccgtgt
     5341 tcttcgcgac ccactcggcg ttctggaagg ccaccgggga gacgtcgcgg cggagctcgt
     5401 cgaggtaggg cgcaacccgg gcgaacagga agatcccctc gccggccgac tccatgaagt
     5461 aatcggcgtt cacgatgccg tgcttcacgg tgccgtagac catctcccag tacgtcgtcg
     5521 tctggcgcca cggcgcgttc agcgggtggt cgaacttcgt gaccgcaagg acgtcctcgt
     5581 aggtcttggg ccagaagtcg cggttgatcg cgttgcgcga ttcgcgcatg acggcctcgc
     5641 ggcgcagctc gtacgcccgg aacgcgagct cggcgtcgtt atggtcggga gtttccttgc
     5701 tcatgccccg actctagcgc aggacgaggc ggcgtctcgg ccggtcagcc ccgcatcggc
     5761 gtgtagcccg tgcaaaagtg ctcgaccatc ttgaggtcca cggtggggcg cggcgtctgc
     5821 ccgttcgacc ggagccggaa gatctcgaac cgcacgccgg gcttcgacac gggccggacg
     5881 ctcaggcggt ggatggcgtc cggcgtgatc gcgtacgagc cgcactcctc gcagtccacc
     5941 gagaacccga cgagggacga cgcgaagagc gtgtgctcgt cgctgatgga cgccggaaag
     6001 ttgcagagga agcacttctc gggcgcaggc gccggggcag gagcgggcgt ctcggccatc
     6061 ggcggagatc ttacgccggg agccgtgtag gcttgcgccc catgagacgt cccgaccccg
     6121 gcctcgtttt cgtcctcccc ctgctcgctt tcgccctcac gcggccgtcg cgcgcccgag
     6181 gggccgatcc gggcgaaagg ccgccgtacc cgcccacgcg cacgacacag gcatccgagg
     6241 tccttcacgg ggtcacggtc atggacccct accggtggct cgaggacgag aagagccccg
     6301 aggtccaggc ctggatgaag gcgcaggacg acttcacgcg ccggacactc gcgtccctgc
     6361 cggagcgcgc cgcgatcgcc gcgcggctca aggagctgct ctacgtcgac agcctcgggg
     6421 ccccgatcca ccgcggcacg cgccatttct tctcccggcg gcacgcggac cgcgagaagt
     6481 cgatcgtgta ctggaaggaa ggcgcaggcg gcgccgagaa cgtcctcttg gacccgaacg
     6541 gctggtccgc ggacggcagc gccgggctcg gtgcctggag cgtctcgtgg gacggcgccc
     6601 gcgtcgccta cggcaagaag gtcaacaatt ccgacgaggc gacgctctac gtgatggacg
     6661 ttgcgaccgg aaaggtctcc gacgtcgacg tgatcgaggg cgcgaagtac gcgtcggcct
     6721 cgtggactcc cgacggcgcc gccttctact acacgaagct ccccgtcgat ccgaagatcc
     6781 ccgcctcaga gcggccgggc tgggcggacg tccgcctgca ccggatcggg accgacccga
     6841 agaccgacac ggtcgtgaaa gcgaagctcg gagacccgac cgcgttccag aacgtccaca
     6901 tcacgcgcga cgggcgcttc ctcttcctcg tggtcgcgca cggctggacg tcgaccgaga
     6961 tctggttccg cgacctcgag acggacccgg ccgcgacggc atggacgccg ctggccacgg
     7021 gcgccagggc gcacttcgac gtcgagacgt tcggccggac gctcttcgtc tcgacggacg
     7081 acggcgcgcc gaagggccgt gtcttcgccg tggaccccgc gaagcccgcc cgcgcggcgt
     7141 ggaaggaaat cgtccccgag cgcaaggacg cggccctgca gagcttctcc gtcgtcggcg
     7201 ggaagctcgc cctcgactac ctgaggagcg ccgcgagcct cctcgaggtc cgcaacctcg
     7261 acgggacgct cctccgcgac gtcccactgc ccggcatcgg gtccgcgagc aacc
//
LOCUS       DAOMFY010000196         7288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3252_length_7288_cov_9.913314, whole genome shotgun sequence.
ACCESSION   DAOMFY010000196 DAOMFY010000000
VERSION     DAOMFY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3252_length_7288_cov_9.913314"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLB01_14040"
     CDS             complement(<1..375)
                     /locus_tag="PLB01_14040"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ78470.1"
                     /translation="MPGAFRRASTLAVIVSAAALARAADAAEDPSALVSRALRAREVR
                     DDATAIRLLEEAVALDRRPEWLGVLAETLAWEKQFARAEALYREALAAAPGSRDLSLG
                     LGRVLLWEGRSLEARAVLAPLAA"
     gene            413..1357
                     /locus_tag="PLB01_14045"
     CDS             413..1357
                     /locus_tag="PLB01_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HQQ78471.1"
                     /translation="MSRTVLIAIGGNSLITDKEHQRVPDQYQACSETCRHIAPLLRDP
                     DLRLVITHGNGPQVGFILRRSELASKELHPVPLDSCVADTQGALGYHLQLALSNEFRR
                     LGLEKAAVTVVTLCLVDRADPAFTKPDKPIGSFLSKEEADLRRATDGWDLVEDAGRGW
                     RRVVASPAPKEILQLDAVKSLLDAGFVVVAAGGGGIAVVEDPAGAIRGAAAVIDKDHA
                     SSLLARRLGAELFVVSTGVAKVSLAFGTPQQKEIDRMTVADARRWMAEGHFKAGSMLP
                     KIEAVIAFLEGGGREAIITDPAHVGLALAGKAGTHVVP"
     gene            1391..2293
                     /locus_tag="PLB01_14050"
     CDS             1391..2293
                     /locus_tag="PLB01_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ78472.1"
                     /translation="MLDVEIDWLYAGPMVQISRYRCHATGCAVGREQRQPAHVIGFPH
                     HGVFALHRGRERLTVEQGTVLFLNHGEPYRTSHPCGGGDHGAAMAVRPDVLVDALARF
                     DPTVADRPAAPFRVAIGATSSRTYLLQRLLHLRVSRRRDGSSLELEEAALALVGDVAA
                     AACRWEGSTPPLRALRERHRDAVHSVRALLAFRLGEPVTLQDVGRAVGVSPFHLCRLF
                     RAVTGTTIARYRHTLRLRAALERVARPDADLSAVALDYGYSSHSHFTAAFRREFGMTP
                     TEFRRAAARSRRNVADLRAPRAGS"
     gene            2356..3018
                     /locus_tag="PLB01_14055"
     CDS             2356..3018
                     /locus_tag="PLB01_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQQ78473.1"
                     /translation="MRKSIRLLALAISAIGACGAALGQAPPPAAPDSAFSWGLKGGVN
                     FANVLLAPDLPPGYSKSSRTGFIGGASAEARLSDTFSVQLEAFYTQKGFEVSSAAGKA
                     TYRLDYLELPLTAKATFGSGPLRPYLFAGPVVGFRLSATAESGSSSGDFKDSTRSTDL
                     GVDLGAGLLYRLNPGTALVLEGRYSVGLVNIFNTGAAGGTVNTRDLKILAGVTFAIGA
                     SR"
     gene            complement(3031..3825)
                     /locus_tag="PLB01_14060"
     CDS             complement(3031..3825)
                     /locus_tag="PLB01_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78474.1"
                     /translation="MSPQRGFPYMRTASAALVAVLLAAPGAPAAPPAQGAPAAKPAPD
                     PTAEEIVARYVTARGGMKKLAALKTLRQEGRINAGGGRDGLVMREIKRPGKIRFEFTV
                     QGITSVFASDGARGWKVNPLEGETSRQPLPEEALVDAREQADIDGPLVDWKSKGSRIE
                     LVGRETVGGHDAWKLKVTLKSGGILTAYVDVKSASLVRTEATRLVRGKRVRIETTLGD
                     YRKTEGILFPHLVEVQAAGRPQRVRIVVDKVEVNPPLSDARFSPPS"
     gene            complement(3822..4520)
                     /locus_tag="PLB01_14065"
     CDS             complement(3822..4520)
                     /locus_tag="PLB01_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78475.1"
                     /translation="MRKLLLALFLTPTLLVADVVYVKGGGSFSGRILEQTAEKVVIDF
                     GDGTIGLQMDQVESIVKGTSPLDEFDARAAKLGPQDVNGWRSLGLWASTKGLSAQSRA
                     AYKRVLALAPDDKEAREALGFVQVDGRWLTEEESYRARGFVKYDGEWMTPAEAQMAQA
                     DAAREQAKDAAARSASDAQFKASMNELQKQEDEKRARDEADRMRNNPVYNPVYIGGWG
                     YGVTSWPNPAGVSR"
     gene            complement(4693..5694)
                     /locus_tag="PLB01_14070"
     CDS             complement(4693..5694)
                     /locus_tag="PLB01_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ78476.1"
                     /translation="MLSALVIAMSFSAPASAQDGGKQASTRFVPVLPEVKARFLSVDP
                     EKGYLVKQVKPDVFVITDGVYQSAFVTTGKGVILFDAPPSFAPHIQQAVADVTGEPIR
                     ELVYSHTHLDHIAGAQHLLTRLPMLEIIAERGVTDFLKEKQDPRRPIPTTTFVGKRTL
                     VLGSAKIELKRGGWHSDEGDLFIYIPDKKFLMAIDTIASGYTPFMDFDLTSNVHRHLK
                     VFDELLAYDFDVLVPGHLTSLATKDDVRVGKEYAVDVYKTVKRIHDATDQKAVMTAAA
                     ATHTWDNKFAIFRTLLDGVTDQCEAEIKGRWIGKLAGVDVWGSSHCRTMLIYARWED"
     gene            complement(5739..6116)
                     /locus_tag="PLB01_14075"
     CDS             complement(5739..6116)
                     /locus_tag="PLB01_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQQ78477.1"
                     /translation="MTIESVLKSYETALNANDIDAILGLYGGDPVFMPQHAPALVGRE
                     AVRAGYAQVFDTIKLTIRFDIHEIHEAGDWAWARTSSAGRTRILAAGVEVEEGNNELF
                     VFRRENGEWRIHRYLFSTSRPRG"
     gene            6226..7140
                     /locus_tag="PLB01_14080"
     CDS             6226..7140
                     /locus_tag="PLB01_14080"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQQ78478.1"
                     /translation="MNQGPDLNAMVLFARVLQHGSFSAAARRAGVPVSTVSRKISNLE
                     RQLGVRLLERTTRAVNPTEAGREYLLHCEQIIDAMESAQAALKERQADVTGTLRLAAP
                     PSLSDVLLVPLVHGFLRSHPKVSVKVLVTDRHLDLVQDEVDISLRVGPQPPSSLVFRP
                     LLRYRHILVAAPAYLAGAHALDKPADLARHRLLGFTKWFDEARWKLSRGTVTERIPVK
                     LWLGMNDYAGVVRAAISGMGVAEIPAILCQQELRQGHLAAVLPKWQFDEVDLSAYYLT
                     RRHPSRMIELFLDYCTANAEGLSRQGSS"
BASE COUNT         1136 a         2485 c         2430 g         1237 t
ORIGIN      
        1 cgcggcgagc ggcgcgagaa ccgcgcgcgc ctcgaggctg cggccttccc agaggaggac
       61 gcgcccgagg cccagcgaaa gatcgcgcga gccgggcgcg gccgcgagcg cctcgcggta
      121 gagggcctcg gcgcgggcga actgcttctc ccacgccagc gtctcggcga ggacgccgag
      181 ccactccggc cgccggtcga gcgcgacggc ctcctcgagg aggcgaatcg cggtcgcgtc
      241 gtcgcgcacc tcgcgcgcgc ggagtgcacg ggaaacgagc gccgacgggt cctcggccgc
      301 gtccgccgcg cgtgcgagtg cggcggccga cacgatgacc gcaagggtgg aagcgcggcg
      361 gaaggctccg ggcacgaccc gtataggcta cccgagaggg aggaagcctc acgtgagcag
      421 gacggtcctc attgccatcg gcgggaactc gctcatcacg gacaaggagc accagcgcgt
      481 ccccgaccag taccaggcgt gctcggagac ctgccgccac atcgcgccgc ttctccggga
      541 cccggatctc cggctcgtga tcacgcacgg caacggcccg caggtgggat tcatactccg
      601 gcggtcggag ctcgcgtcga aagagctgca ccccgtgccc ctggactcgt gtgtcgcgga
      661 tacccagggc gcgctcgggt accacctcca gctcgcgctc tcgaacgagt tccggcgcct
      721 cggcctcgag aaggcggccg tcacggtggt cacgctctgc ctcgtggacc gggccgatcc
      781 cgcgttcacg aagccggaca agcccatcgg gtcgttcctg tcgaaggaag aggccgatct
      841 ccgccgcgcg acggacgggt gggacctggt cgaggacgcg gggcgcggct ggaggcgcgt
      901 cgtcgcctcg ccggccccga aggagatcct ccagctcgac gccgtgaaga gcctgctcga
      961 cgccggcttc gtcgtcgtcg cggcgggcgg aggcgggatc gcggtggtcg aggacccggc
     1021 gggcgcgatc cggggcgcgg ccgccgtcat cgacaaggac cacgcgtcga gcctcctcgc
     1081 gcgccggctg ggcgccgagc tcttcgtcgt ctcgacgggc gtcgcgaagg tctcgctcgc
     1141 cttcggaacg ccgcagcaga aggagatcga ccggatgacc gtcgcggacg cgcggcgctg
     1201 gatggccgag ggccacttca aggcggggag catgctcccg aagatcgagg ccgtgatcgc
     1261 cttcctggag ggtggcggac gcgaggcgat catcaccgac ccggcccacg tcggcctcgc
     1321 gctcgccggc aaggcgggca cccacgtcgt gccgtgaaac ccgacggcgc ccgtcccgta
     1381 gtcgtgggtg atgctcgacg ttgagatcga ctggctctac gccgggccga tggtccagat
     1441 ctcgcgatac cgatgccacg cgacaggttg tgccgtcgga agagagcagc gacagccggc
     1501 ccacgtcatc ggctttcccc accacggcgt cttcgcgctt caccggggcc gtgagcgcct
     1561 cacggtcgag cagggcacgg tcctcttcct caaccacggc gagccctatc gcacgagcca
     1621 tccctgcggg ggcggcgacc acggcgcggc gatggccgtc cgcccggacg tcctcgtcga
     1681 cgcgctcgcg agattcgacc cgacggtggc ggaccgcccg gcggcgccgt tccgcgtcgc
     1741 gatcggcgcg acctcctcgc gcacgtacct gctccagcgg cttctccacc tccgcgtctc
     1801 gaggaggcgc gacgggagct cgctcgagct cgaggaagcc gcgctggcgc tcgtcggtga
     1861 cgtcgcggcg gcggcctgcc gatgggaggg aagcacgccc ccgctccggg ccctccggga
     1921 acgccaccgc gacgccgtcc attcggtgcg cgccctgctc gccttcaggc tcggggagcc
     1981 ggtgactctc caggacgtcg gccgggcggt cggggtctcg cccttccatc tctgccggct
     2041 cttccgggcg gtcaccggga cgacgatcgc ccgctaccgc cacacccttc gcctgcgggc
     2101 cgcgctcgag cgggtggccc gaccggatgc cgacctgagc gccgtcgccc tcgactacgg
     2161 ctactccagc cacagccact tcacggccgc gttccggcgg gagttcggca tgacgccgac
     2221 ggaattccgg cgcgcagccg cgcggagccg gcggaacgtc gcggacctgc gcgcgccccg
     2281 agcaggatcc tgacagcgcg gggcgccggc gcgccccatc gtgcccgcag tccatgtcgg
     2341 acgtggagga ctcgcgtgcg aaaatccatc cggctcctgg ccctggcgat ctcggcgatc
     2401 ggcgcgtgcg gcgcggcgct gggtcaggcg ccgcctccgg cggctccgga ctcggcgttt
     2461 tcgtggggcc tgaagggcgg cgtcaatttc gcgaacgtcc tgctcgcccc ggaccttccg
     2521 ccgggatact cgaaatcctc ccggacgggc ttcatcgggg gcgcgtctgc cgaggcccgg
     2581 ctctcggaca cgttctcggt tcagctcgag gccttctaca cgcagaaggg attcgaggtc
     2641 tcgtcggccg ccggcaaagc gacgtaccgc ctcgactacc tggaactgcc cctcaccgcg
     2701 aaggcgacgt tcgggtcggg cccgctccgg ccatatctct tcgccgggcc ggtcgtcggc
     2761 tttcgtctct ccgctaccgc ggagagcgga tcgagttccg gtgacttcaa ggactcgacg
     2821 aggtcgacgg atctgggcgt cgacctgggt gcgggccttc tctatcggct gaatccgggg
     2881 acggcgctcg tcctcgaagg ccgctactcg gtgggcctcg tgaacatctt caacaccgga
     2941 gccgcgggcg gaacggtgaa cacgcgggac ctgaagatcc tcgcgggcgt gaccttcgcg
     3001 atcggcgcgt cgcgctagcc ctcgttcgcg tcaggacggc ggggagaagc gcgcgtcgct
     3061 cagcggcgga ttcacctcga ccttgtccac cacgatccgg acccgctgcg gccggccggc
     3121 ggcctggacc tcgacgaggt gggggaagag gatcccttcc gtcttccggt agtcgcccaa
     3181 cgtcgtttcg atccggaccc gcttcccgcg cacgagccgc gtggcctcgg tccggacgag
     3241 gctcgcggat ttcacgtcga cgtaggcggt gagaatcccg ccgctcttga gggtgacctt
     3301 cagcttccag gcgtcgtggc cgccgacggt ttcgcggccg acgagctcga tccggcttcc
     3361 cttgctcttc cagtcgacga gcggcccgtc gatgtcggcc tgctcccgcg cgtcgaccag
     3421 ggcctcctcc ggcaggggct ggcggctcgt ctctccttcg agcgggttca ccttccagcc
     3481 gcgcgcgccg tcggaagcga agacgctcgt gatgccctgg acggtgaact cgaaccggat
     3541 cttcccggga cgcttgatct cgcgcatcac gagcccgtcg cggccgcccc cggcgttgat
     3601 ccgcccttcc tggcgcagcg tcttcagggc cgcaagcttc ttcatccctc cccgtgcggt
     3661 cacgtagcgc gcgacgatct cttcggccgt cgggtcgggt gcgggtttcg cggcgggcgc
     3721 accttgcgcg ggaggggcgg ccggggcgcc gggcgccgcc agcaggaccg cgacgagggc
     3781 cgccgacgcg gtgcgcatgt agggaaatcc gcgttgcggg ctcatctcga aactcctgcg
     3841 ggattcggcc aggacgtcac gccatagccc caccccccga tgtagacggg gttgtaaacg
     3901 gggttgtttc tcatccggtc cgcttcgtcc cgggcgcgct tctcgtcttc ctgcttctgc
     3961 agctcgttca tgctcgcctt gaactgcgcg tccgaggcgc tcctggcggc cgcgtccttc
     4021 gcctgctcgc gcgcggcgtc ggcctgcgcc atctgcgcct cggcgggcgt catccactcg
     4081 ccgtcgtact tgacgaagcc ccgggcccga tagctctcct cttcggtgag ccagcggccg
     4141 tcgacctgca cgaaccccag cgcctcgcgg gcttccttgt cgtcgggagc gagcgcgagg
     4201 acccgcttgt aggccgcgcg cgactgggcc gaaagcccct tcgtcgaagc ccacagaccg
     4261 aggctgcgcc agccgttgac gtcctgcggg cccagcttgg ccgcccgcgc gtcgaactcg
     4321 tcgagcggcg aggtgccctt cacgatgctc tcgacctggt ccatctggag accgatggtg
     4381 ccgtcgccga agtcgatgac gaccttctcc gccgtctgtt cgaggatccg gccggaaaaa
     4441 ctgccgcccc ccttgacgta cacgacgtcc gcgacgagga gcgtaggtgt cagaaagaga
     4501 gccaggagaa gctttctcac ggaagtcctc tggcgccagt ctgggatcgg ccggagggcg
     4561 ctgtcaaccg cggtgcggcg ggccgggcgt cgatctggcg gtaacgaaaa ggcccggtgt
     4621 ccttcttgga agacgctgac cgcgatgatg gcgaccaaac cagtcgcacg gcgcaactcc
     4681 atcgctgatc gcttagtcct cccaccgggc gtaaatcagc atcgttctgc agtggctcga
     4741 tccccacaca tcgacgccag cgagcttacc gatccaacgg cctttgattt cagcctcgca
     4801 ctggtcggtc acgccgtcga ggagagttct aaagatcgcg aacttgttat cccaggtgtg
     4861 ggtcgcagcc gccgccgtca tcaccgcctt ctgatcggtc gcgtcgtgaa ttcgcttgac
     4921 ggtcttgtag acgtcgacgg catattcctt gccaacacgc acatcgtcct tcgtggcgag
     4981 ggaggtcagg tgcccgggca cgagcacatc gaagtcgtag gccaggagtt cgtcgaacac
     5041 cttcaggtgt cgatggacat tcgacgtcag gtcgaagtcc atgaagggcg tgtagccaga
     5101 tgcgatcgtg tcgatggcca tcaggaactt cttgtcaggg atgtagatga acagatcacc
     5161 ctcgtccgag tgccagcccc ctcgcttcaa ctcgattttg gccgaaccca gcacgagtgt
     5221 tcgcttgccg acgaacgtgg tcgttgggat gggccgccgc ggatcctgct tttccttcag
     5281 gaagtccgtg acaccacgtt cggcgatgat ctccagcatc gggagtctcg tgagcagatg
     5341 ctgggcgccg gcgatgtggt cgaggtgcgt gtgcgagtac acgagctcgc gaatcggctc
     5401 cccggtcaca tcggcgaccg cttgctgaat gtgcggtgca aacgacggcg gagcgtcaaa
     5461 caagatgacg cccttgccgg tcgttacgaa tgcggactga tacaccccgt ctgtgatcac
     5521 gaacacgtcc ggcttgacct gcttgacgag ataccccttc tcgggatcca cagaaagaaa
     5581 gcgagccttc acctccggta ggacgggtac gaacctagtg cttgcttgct tgccgccgtc
     5641 ttgcgcagaa gccggggcac tgaagctcat cgcgatgacg agcgcgctga gcacgcggct
     5701 gattcgcatc gtacaagtcc ctgagaacgc gaagccgttc atccgcgcgg acggctggtc
     5761 gagaacaggt agcggtggat cctccactcg ccattctctc gacggaacac gaacagctcg
     5821 ttgttgcctt cctcgacctc gacgccggcc gcgaggatcc gtgtccgacc agccgagctg
     5881 gtacgggccc aggcccagtc ccccgcctcg tggatctcgt gaatatcgaa gcgaatggtc
     5941 agcttgatcg tgtcgaatac ctgcgcgtaa ccggcgcgca ccgcttcgcg accgacgagc
     6001 gcgggcgcat gctgcggcat gaacacgggg tcgccgccgt acagccccag aatcgcgtcg
     6061 atatcgttgg cgttgagtgc ggtttcgtag gacttgagaa cgctctcgat ggtcatgggc
     6121 tcgctcctta ttgcagcctt tccggcggct cgcctcgtaa cgtaacgtcc tcgcgtattc
     6181 gagacaatag acatgtctgg aaaggattat tgcgataatg gaaggatgaa tcagggcccc
     6241 gacctcaatg cgatggtgct cttcgcgcgc gtccttcagc acggcagctt ctcggcggct
     6301 gcgcggcgcg caggcgttcc tgtctcgacg gtcagccgga agatcagcaa cctggagcgc
     6361 cagcttggtg tccgactgct ggaacggacg acgagggccg tcaatccaac cgaagccggc
     6421 cgcgagtacc tgctgcactg cgaacagatc atcgatgcga tggagagcgc gcaagctgcg
     6481 ttgaaggagc ggcaggccga tgtgacggga acgctccggc tcgcggcgcc gccgagcctg
     6541 tcggacgtcc tgctcgtccc tttggtccac gggttccttc gaagtcatcc gaaggtctcc
     6601 gtcaaggtgc tggtcaccga ccggcacctt gacctggtgc aggacgaggt ggacatctcg
     6661 ttacgggtcg gacctcaacc tccgagcagc ctcgtgttcc gaccgctcct gcgataccgg
     6721 cacatcctcg tcgccgcccc ggcatatctc gccggggcgc atgctctgga caaacccgct
     6781 gacctcgcgc gccaccggct cctcggtttc accaaatggt tcgacgaggc cagatggaag
     6841 ctgtcgaggg gcaccgtcac cgagcgcata ccggtgaagc tctggttggg catgaacgac
     6901 tacgccggcg tcgtccgggc ggccatctcg ggaatgggag tggcagagat tcctgccatc
     6961 ctgtgccaac aggagttgcg tcaagggcac ctggcggctg tcttgccgaa atggcagttc
     7021 gacgaggtgg atctgtccgc gtattacctc actcggcgtc atccttcacg gatgattgag
     7081 ctgtttctcg actactgcac ggcaaacgca gagggtctct cgcgccaggg ctcgtcctga
     7141 ccgccacctc caggaaatcg gacacgaaca gaaggcggac aaagaggccc gcgaaaagaa
     7201 aaacgggtta gcccgatcga gctaacccgt tgattctatt ggttgcgggg gcaagatttg
     7261 aacttgcgac ctttgggtta tgagggcg
//
LOCUS       DAOMFY010000197         7285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3256_length_7285_cov_7.184924, whole genome shotgun sequence.
ACCESSION   DAOMFY010000197 DAOMFY010000000
VERSION     DAOMFY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_7285_cov_7.184924"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLB01_14085"
     CDS             <1..889
                     /locus_tag="PLB01_14085"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQQ78479.1"
                     /translation="SEVSMKKILLASGMLSLLLGAALVAGPASGPAAVRPQLAGKFVW
                     FDLLTNDAAAAEKYYGGLFGWTFEVLAGRENPYKVIREKGQPIGGIVDMSARKSDLPE
                     STWLGYVSVPDVDAAAAAFKAKGGAVLKGPLDVTNLARAAVVTDPQRALLGLIRIAKG
                     DPPDAPPADGRFLWMEHMAKDAPGAMTFYRDVLGYDAKPLDAGVAGVDFPYFVLGKGG
                     KNRAGLYVTPWKELNSNWLPYVKVADAGAAAEKAKSLGGRVVLAPRADLRDGSLAIVV
                     DPTGAALALQKYPFEKNGK"
     gene            903..1118
                     /locus_tag="PLB01_14090"
     CDS             903..1118
                     /locus_tag="PLB01_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78480.1"
                     /translation="MKKTLSRALALAGLLALAVTASGCYPYEETGDPYGYSPVGVSVS
                     VYGGYYSPGWGGCCIGGPIGMPRPMPY"
     gene            complement(1112..4282)
                     /locus_tag="PLB01_14095"
     CDS             complement(1112..4282)
                     /locus_tag="PLB01_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase"
                     /protein_id="HQQ78481.1"
                     /translation="MRSAALLLAFSLAASAAPPAGDAGTISGLGARNIGSAQMSGRIA
                     ALAGRVEDDGKVTLFVGAASGGVWKSVDGGTTFKPVFDRQPVQSIGAIALDPSDPKVV
                     WVGTGESWTRNSTSVGDGVYRSGDGGETWKNVGLAGSERVNRILVHPKKSDVVYACVP
                     GRLWSDSADRGLYKTTDGGKSWSLVLKGGNLSTGCSGLAMDPRNPDRLFAGLWDFRRK
                     GWTFRSGSEGPKGTSGSGLFLTEDGGATWKTLDARSAPGLPEGPWGRLDVAIAPSKPD
                     RVYAVIEGVKSALFRSDDGGKTWARKDDSQRMVWRPFYFSRLIVDPTNADRVYKPNLT
                     LIASEDGGESFGDVGGGTHGDHHDVWINPKNPKHVVTADDGGLWISHDGANRWWKCNN
                     LPVSQFYHVSVDEKSPYQVYGGLQDNSSWAGDSAYPGGITNGRWENLYNGDGFWVFVD
                     PADPNFVYAEAQGGFIGRIDRRTLAARDIQPKALYKEKLRWNWNTPIHVSRNEKGKLY
                     IGAQYLFATRDGGQSWQRLSPDLTTNDPEKQKQEQSGGITVDNSAAEMHTTIYSISES
                     PRDARTIWVGTDDGNLQLTKDGGTTWTNLVKNVPGLPAGSWVSWVEAGPHADGAAYAA
                     FDRHTFGDMDPWVYATRDSGKTWERIVGPSSGVRGWAYVIKEDPVRPGLLYLGTELGL
                     WISLDAGKTWAEFRGGNFPAVSVRDLAFQTRDHDLVVATHGRGIWIVDDLTPLRALTP
                     ETLAKDLAFLPGRPVVQKPRGQGGWPEGDATFVGPNPPAGAAVSYYQRTRHTFGRMKL
                     EVLDASGRVVDTLPAGKRKGINRVIWSMQEKPPRVPTAAQAGGAGAGPRVLPGTYTVR
                     LTKGKDVVEAPLVVSLDPKAPFTLDDRKAQYDAAMRAHALFGRMSDVVDRLNGYRALA
                     AARAKGLPAGDALRRDLDAFAAKADTIRKDVVATSEGGAITGEERLREHLAYAYDAIL
                     SYEGRPGDYQVERVSVLERELKDVEARAADLAAKDLPALNGKLKAKKLAPIADAAARD
                     EGARIAAARTIEHAWAVAREGAAARQ"
     gene            complement(4279..5052)
                     /locus_tag="PLB01_14100"
     CDS             complement(4279..5052)
                     /locus_tag="PLB01_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78482.1"
                     /translation="MRSRRFFSFIGVFFSLSLSSPALAITAEEIVAKNVEARGGAAAL
                     GSLKSLRRTGQFVIPGRNLLITTTDLKERPGRVRQEATYQGLTQIQAWDGERGWQVQP
                     FEGRKEASLMSEDDVKPFRLAADLDGPFVNAKEKGHTLEYLGTEDVDGTLAHKLRVRL
                     KWGGEILVYIDPDTWMVIRDVQKNVVRGAEQEIETDYGDYEKVGGVYVAMSEESGPAG
                     SPPSSRGKSVYDKAEASVAVEADAFAFPARIAAPAGAGR"
     gene            5128..5376
                     /locus_tag="PLB01_14105"
     CDS             5128..5376
                     /locus_tag="PLB01_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQQ78483.1"
                     /translation="MPILEFRCAGCEKTSEVLVLAGDEAPEASCPACGSRDLTRLLST
                     FAAHAGSGRARTAETACGGGPCAMPGMCGPSACGFGEN"
     gene            5379..5837
                     /locus_tag="PLB01_14110"
     CDS             5379..5837
                     /locus_tag="PLB01_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQQ78484.1"
                     /translation="MLGIKRETDYAVRTVLHLASLPPGAQVQVKDVAAQKLLPLSFVR
                     RIVARLGAAGLLATTRGLKGGISLARPASEISLLDVVLAMDDPVTLNQCLDPDHTCPL
                     SAACPAHVAWAEATDMLETHLASVRFDALAGGERHAGAHRRLARTNTRRS"
     gene            5847..6494
                     /locus_tag="PLB01_14115"
     CDS             5847..6494
                     /locus_tag="PLB01_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78485.1"
                     /translation="MTFRTTALSALIAATTAFPGLAAGLDPATQARLAADKEAIRQAD
                     ASRRSVLLTRGTDQGERNRALEDFRDRRRKAVLDAVEALLAVRATVPKEEWKAIVAQV
                     GAGGGMPFLAGKAQKELPAVVLDPARLASADKTLADLAAALKKQGADPNSAGQRFLSL
                     LEKKNSTKDDFITAMEKLTAAQEKLDQRVTDGISNLQRTLTPEEWDELVRRLAAP"
     gene            6577..>7285
                     /locus_tag="PLB01_14120"
     CDS             6577..>7285
                     /locus_tag="PLB01_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQQ78486.1"
                     /translation="MQDSFKTRASLKAGSATYNYYSLKTLEKKFPRVKKLPFSLRILL
                     ENLLRHEDGRTVTKEDIEALANRDVKKPTDREIAFRPARVLLQDFTGVPAVVDLAAMR
                     EALAAMGGNPEKINPLAPADLVIDHSVIVDEFATSQAFGNNAKLEFDRNRERYAFLRW
                     GQNAFRNFRVVPPDTGICHQVNLEYLASVVFTGAGKMAYPDTLVGTDSHTTMINGLGV
                     LGWGVGGIEAEAALLGQP"
BASE COUNT         1052 a         2660 c         2462 g         1111 t
ORIGIN      
        1 atcggaggtt tccatgaaga agatcctcct cgcatccggc atgctctcgc tgctcctcgg
       61 ggccgcgctc gtggccgggc cggcgtcggg tccggcggcc gttcggccgc agctcgccgg
      121 gaagttcgtg tggttcgacc tcctcacgaa cgacgcggcg gccgccgaga agtactacgg
      181 cggtctcttc ggctggacgt tcgaggtcct cgccgggcgc gagaacccct acaaggtgat
      241 ccgcgagaag ggccagccga tcggcgggat cgtggacatg tcggcccgca agtcggacct
      301 gcccgaatcc acctggctcg gctacgtctc ggttccggac gtggacgccg cggcggccgc
      361 cttcaaggcg aagggcggcg ccgtcctgaa gggaccgctc gacgtcacga accttgcccg
      421 cgcggccgtc gtcacggacc cgcagcgcgc cctcctgggc ctgatccgca tcgcgaaagg
      481 cgatcctccg gacgctcccc ccgcggacgg ccgcttcctg tggatggagc acatggccaa
      541 ggacgcgccg ggggcgatga cgttctaccg cgacgttctc ggatacgacg ccaagccgct
      601 cgacgcgggc gtcgccggcg tcgacttccc gtacttcgtg ctcgggaagg gcggaaagaa
      661 ccgcgcggga ctctacgtga cgccctggaa ggagctgaac tcgaactggc ttccctacgt
      721 caaggtcgcg gacgcgggcg cggcggcgga gaaggcgaag tctctcggcg gccgcgtcgt
      781 gctcgcgccg cgcgcggatc tccgcgacgg cagcctcgcg atcgtcgtcg accccacggg
      841 ggcggccctc gcgctccaga agtacccctt cgagaagaac ggaaagtgac gaggaggatg
      901 cgatgaagaa gaccctctcc cgcgcgctcg cgctcgccgg cctcctcgcg ctcgcggtca
      961 ccgcctccgg ctgctacccc tacgaagaga cgggcgatcc ctacggctac tcgccggtcg
     1021 gcgtgagcgt gagcgtctac ggcggctact actccccggg ctggggaggc tgctgcatcg
     1081 gcggcccgat cgggatgccc cggcccatgc cctactgacg cgccgcggcg ccctcgcggg
     1141 cgacggccca cgcgtgctcg atcgtccgcg ccgccgcgat ccgcgcgccc tcgtcccggg
     1201 cggccgcgtc cgcgatcggc gcgagcttct tcgccttcag cttcccgttg agggccggga
     1261 ggtccttcgc ggcgaggtcc gcggcgcgcg cctcgacgtc cttcagctcg cgctcgagca
     1321 cggacacgcg ctcgacctgg tagtcgcccg gccgcccctc gtacgagagg atcgcgtcgt
     1381 aggcgtacgc gaggtgctcg cggaggcgct cctctcccgt gatcgcgccg ccctcggacg
     1441 tcgcgacgac gtccttgcgg atcgtgtcgg ccttcgcggc gaacgcgtcg aggtccctgc
     1501 ggagcgcgtc tcccgccggg aggcccttcg cgcgcgccgc cgcgagcgcg cggtagccgt
     1561 tcaggcggtc cacgacgtcg ctcatgcgcc cgaagagcgc gtgcgcccgc atcgcggcgt
     1621 cgtactgggc tttgcggtcg tcgagcgtga agggcgcctt cgggtcgaga gagacgacga
     1681 gcggcgcctc cacgacgtcc tttcctttcg tcaggcgcac cgtgtacgtg ccggggagga
     1741 cgcgaggccc tgcgccggcg ccgcccgcct gagcggccgt cgggacgcgc gggggcttct
     1801 cctgcatcga ccagatgacg cggttgatcc ctttcctctt gcccgcgggc agggtgtcga
     1861 ccacgcggcc cgaagcgtcg aggacctcga gcttcatccg cccgaacgtg tgccgcgttc
     1921 tctggtagta cgaaacggct gcgcccgccg gcgggttcgg cccgacgaac gtcgcgtcgc
     1981 cctcgggcca tccgccctgg ccgcgcggct tctggacgac cggacgcccc gggaggaacg
     2041 cgaggtcctt cgcgagcgtc tcgggcgtca gcgcgcggag cggcgtgagg tcgtcgacga
     2101 tccagatccc gcgcccgtgc gtcgcgacga cgaggtcgtg gtcgcgcgtc tggaacgcga
     2161 ggtcgcgcac ggacacggcc gggaagttcc cgcccctgaa ttccgcccac gtcttcccgg
     2221 cgtcgagaga gatccagagg cccaactccg tgccgagata gaggaggccg ggccggacgg
     2281 gatcctcctt gatcacgtag gcccagccgc ggacgcccga ggacgggccc acgatccgtt
     2341 cccacgtctt gccggagtcc ctggtcgcgt acacccacgg atccatgtcg ccgaaggtgt
     2401 gccggtcgaa cgccgcgtac gcggcgccgt cggcgtgcgg gcccgcctcc acccacgaga
     2461 cccagctccc ggccggaagg ccggggacgt tcttcacgag attcgtccag gtcgtcccgc
     2521 cgtccttcgt gagctggagg ttcccgtcgt cggtccccac ccagatcgtc ctcgcatccc
     2581 gcgggctctc cgagatcgag tagatcgtcg tgtgcatctc cgcggcggag ttgtccaccg
     2641 tgatgccgcc cgactgctcc tgcttctgct tctccgggtc gttcgtcgtc aggtccggcg
     2701 acaggcgctg ccacgactgg ccgccgtccc tcgtcgcgaa gaggtactgg gcgccgatgt
     2761 agagcttgcc cttctcgttc cgcgagacgt gaatcggcgt gttccagttc cagcgcagct
     2821 tctccttgta gagcgccttc ggctggatgt cgcgggccgc gagcgtgcgt cggtcgatgc
     2881 ggccgatgaa gcctccctgc gcctccgcgt acacgaagtt cgggtcggcg gggtccacga
     2941 agacccagaa gccgtcgccg ttgtagaggt tctcccagcg gccgttcgtg atgccgcccg
     3001 gatacgcgga gtcgccggcc cacgagctgt tgtcctgcag gccgccgtag acctggtacg
     3061 gagacttctc gtccaccgac acgtggtaga actgagagac gggaaggttg ttgcacttcc
     3121 accagcggtt cgcaccgtcg tgggagatcc agagaccgcc gtcgtcggcc gtcacgacgt
     3181 gcttcgggtt tttcgggttg atccagacgt cgtggtggtc cccgtgcgtg ccgccgccca
     3241 cgtcgccgaa gctctcgcct ccgtcctcgg acgcgatgag cgtgaggttc ggcttgtaga
     3301 cgcggtcggc gttcgtcggg tcgacgatga ggcgcgagaa atagaacggc cgccagacca
     3361 tccgctggct gtcgtccttg cgcgcccacg tctttccgcc gtcgtcggag cggaacagcg
     3421 cggacttaac gccctcgatc acggcgtaaa cgcggtcggg cttcgacggc gcgatcgcga
     3481 cgtcgaggcg cccccacggc ccctcgggca ggccgggagc gctccgggcg tcgagcgtct
     3541 tccacgtcgc gccgccgtcc tccgtgagga aaaggccaga cccggaagtc cccttcggcc
     3601 cttcactccc tgaccgaaaa gtccagcctt tccgccggaa atcccacagg cccgcgaaga
     3661 ggcggtccgg gttgcgcggg tccatcgcga ggccggaaca gcccgtcgag aggttgccgc
     3721 ccttgaggac gagcgaccag gacttcccgc cgtccgtcgt cttgtagaga ccgcggtccg
     3781 cggagtccga ccagagcctg ccgggcacgc atgcgtacac gacgtcgctc ttcttcgggt
     3841 gcacgaggat ccggttcacg cgctcggatc ccgcgaggcc cacgttcttc caggtctcgc
     3901 cgccgtcgcc ggagcggtag acgccgtctc ccaccgacgt ggagttgcgc gtccagctct
     3961 cgccggtgcc cacccagacg accttcgggt cggaggggtc gagcgcgatc gcgccgatgg
     4021 actggacggg ctgcctgtcg aagacgggtt tgaacgtggt cccgccgtcg accgacttcc
     4081 agacgccgcc cgaggcggcg ccgacgaaga gcgtgacttt gccgtcgtcc tcgacgcggc
     4141 ccgcgagcgc ggcgatgcgc ccgctcatct gggccgagcc gatgttgcgc gcgccgaggc
     4201 ccgagatcgt tccggcgtcg cccgcgggcg gcgcggcgga cgcggcgagc gagaacgcga
     4261 gcagaagagc ggcgcttctc atcggcccgc tcccgccggc gcggcgatcc gtgccgggaa
     4321 cgcaaacgcg tccgcctcga cggcgacgct ggcttcggcc ttgtcgtaga cggacttgcc
     4381 ccgagaggac ggcggcgaac ccgccggccc gctctcctcg ctcatggcga cgtagacgcc
     4441 gcccaccttc tcgtagtcgc cgtagtccgt ctcgatctcc tgctcggccc cgcgaacgac
     4501 gttcttctgc acgtcgcgga tcaccatcca ggtgtcgggg tcgatgtaga cgaggatctc
     4561 gcctccccac ttcaggcgca cgcgcagttt gtgcgcgagc gtgccgtcca cgtcctcggt
     4621 cccgaggtac tcgagcgtgt ggcctttctc cttcgcgttc acgaacggcc cgtcgaggtc
     4681 ggccgcgagc cggaacggct tgacgtcgtc ctcggacatg agcgaggcct ccttgcggcc
     4741 ctcgaagggc tgcacctgcc agcccctctc gccgtcccac gcctggatct gcgtgaggcc
     4801 ctggtacgtg gcctcctgcc ggacgcgccc cgggcgttcc ttgaggtccg tcgtcgtgat
     4861 gagaaggttg cgccccggga tcacgaactg gccggtgcgg cggaggctct tcagggaccc
     4921 gagggcggcg gcgcccccgc gggcctcgac gttcttcgcg acgatctcct cggccgtgat
     4981 cgccagggcg ggagacgaga gagagagcga gaaaaagact ccaatgaagg agaagaaccg
     5041 gcggctccgc atgtccgtcc tacctttcga agaagtcatc ttaggccgta ccccccagtg
     5101 taatcttcac tccagggacg aaagctcatg cccatcctcg aattccgttg cgcgggctgc
     5161 gaaaagacct cggaggtgct cgtcctcgcg ggcgacgagg ccccggaggc gtcctgcccg
     5221 gcctgcgggt cgcgggacct gacgcgcctc ctgtccacgt tcgccgcgca cgccggctcg
     5281 ggccgggcgc ggacggcgga gaccgcctgc ggcggcggcc cctgcgccat gccggggatg
     5341 tgcggcccgt cggcgtgcgg cttcggcgag aactgagcgt gctgggcatc aaacgcgaaa
     5401 cggattacgc ggtccggacc gtcctgcacc tcgcatccct tcctcccggc gcccaggtgc
     5461 aggtcaagga cgtcgccgcg cagaagctgc tcccgctgtc cttcgtgcgg cgcatcgtcg
     5521 cgcgcctcgg cgcggccgga ctcctcgcga cgacgcgcgg cctcaagggc gggatctccc
     5581 tcgcgcggcc ggcctcggag atctcgctcc tcgacgtcgt cctcgcgatg gacgaccccg
     5641 tgacgctcaa ccagtgtctc gacccggacc acacgtgccc gctctccgcg gcgtgtcccg
     5701 cgcacgtcgc gtgggccgag gcgacggaca tgctcgaaac gcacctcgcc tccgtacgct
     5761 tcgacgctct cgccggaggc gagcgccatg ccggcgccca ccgccggctc gcccgcacga
     5821 acacacgccg ctcctaagga gacagaatga ccttccgcac gacggccctt tcggccctta
     5881 tcgccgccac gaccgccttc cccggcctcg cggccggcct cgacccggcg acgcaggcgc
     5941 ggctcgcggc cgacaaggag gcgatccgac aggccgacgc gtcgcggcgg tccgtcctcc
     6001 tgacccgggg caccgaccag ggggaacgca accgcgcgct cgaggacttc cgcgaccggc
     6061 ggcgcaaggc ggtcctcgac gccgtcgagg cgctcctcgc cgtgcgcgcc accgtcccga
     6121 aggaggagtg gaaggcgatc gtcgcccagg tcggcgcggg cggggggatg ccgttcctcg
     6181 ccggaaaggc gcagaaggag ctgcccgccg tcgtcctcga cccggcccgt ctggcctccg
     6241 ccgacaagac cctcgcggac ctcgcggccg ccctgaagaa gcagggggcg gacccgaact
     6301 cggccggcca gaggttcctc tccctcctcg agaagaagaa ctcgacgaag gacgacttca
     6361 tcacggccat ggagaagctc accgcggccc aggaaaagct cgaccagagg gtcacggacg
     6421 gcatcagcaa cctccagagg accctgaccc ccgaggagtg ggacgagctc gtccggcggc
     6481 tcgcggctcc ctgatccgtc tgaatcaggg ctgtcccggc aatcctgcgt ctagaatgcc
     6541 ccgcccgccg gttccggcgg gactgccgga ggacacatgc aggacagttt caagaccagg
     6601 gccagcctga aggccggctc ggcgacctac aactactaca gcctcaagac gctcgagaag
     6661 aagttcccca gggtcaagaa actcccgttc tcgctccgga tcctcctcga gaacctcctc
     6721 cgccacgagg acggccggac cgtcacgaag gaagacatcg aggcgctcgc gaaccgcgac
     6781 gtgaagaagc cgacggaccg cgagatcgcg ttccggcccg cgcgcgtcct cctccaggac
     6841 ttcaccggcg tcccggccgt cgtcgacctc gcggcgatgc gcgaggcgct cgccgccatg
     6901 ggcggcaacc ccgagaagat caacccgctc gccccggccg acctcgtgat cgaccactcc
     6961 gtcatcgtcg acgagttcgc gacgtcgcag gcgttcggga acaacgcgaa gctcgagttc
     7021 gaccgcaacc gcgagcgcta cgcgttcctc cgctggggcc agaacgcgtt ccgcaacttc
     7081 cgcgtcgtcc cgcccgacac ggggatctgc caccaggtga acctcgagta cctcgcgagc
     7141 gtcgtcttca ccggcgcggg caagatggcg tacccggaca ccctcgtcgg gaccgacagc
     7201 cacacgacga tgatcaacgg gctcggcgtc ctcggctggg gcgtgggcgg gatcgaggcc
     7261 gaggccgcgc tcctcggcca gccgc
//
LOCUS       DAOMFY010000198         7270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3270_length_7270_cov_7.125295, whole genome shotgun sequence.
ACCESSION   DAOMFY010000198 DAOMFY010000000
VERSION     DAOMFY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_7270_cov_7.125295"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLB01_14125"
     CDS             <1..349
                     /locus_tag="PLB01_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ78487.1"
                     /translation="RPNELSGGQRQRVAFARALAVNPRVLLLDEPFGALDAGVRLELG
                     EWLLRLHDETGLTTLLVTHDQPEAFALASHLVVLFDGKVAQAGTPAELRANSADDRLA
                     AFLRVVRRSPAAG"
     gene            383..3055
                     /locus_tag="PLB01_14130"
     CDS             383..3055
                     /locus_tag="PLB01_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HQQ78488.1"
                     /translation="MPKSGPLDPESLRLEENRSRKANWKRWGPYLAERQWGTVREDYG
                     AEGQPWTYFPHDHARSRAYRWGEDGIAGFSDRHGLICLAPTLWNGRDPILKERLFGLS
                     GPEGNHGEDAKEYWFTLDATPTHAYMRMLYKYPQREFPYRALVEENARRTRLDPEFEL
                     LDTGVFEESRYWDVFVTYAKASPEDLLVTIEAVNRGPEAAPLHVLPTIWFRNTWSWGL
                     DARLPRLAAAAPPDGAVAIAAHHPYYGDRTLYVEGAPDLLFTDNITNAALLFGAENEG
                     RFVKDAFHARVVQGVEDAVNPAGYGTKAAAWFRFDVPAGESRKVRLRLSDRALAAPFG
                     EDFDRTVAARRSEADAFYARVIPPELSADAKNVMRQSLAGMLWSKQYYGYDVARWLKG
                     DPGQPAPPPGREQGRNAGWRHLFNADVLSMPDKWEYPWYAAWDLAFHTIPLALVDPDF
                     AKDQLILLLREWYMHPNGQLPAYEWKFEDVNPPVHAWAALRVYKIDEKRSGTPDREFL
                     ARVFHKLLLNFTWWVNRKDENGNNVFQGGFLGLDNIGLFDRSKPLPLGATLEQSDATS
                     WMGMYCLNMGTIALELARADRAYEDIASKFFEHFMYISKAMNAPEHGSGLWDEADGFF
                     YDVLRLADGHGIPMRIQSMVGLIPLFAVATLEPYVLEAFPGFRRRMQWFLDHGSAYGA
                     KVETQATAAGTRRLLSLVGRERLRRILEPLLDPDGFLSDHGVRSLSRAHRDRPFVVPW
                     DGRELRVDYEPAESRTSLFGGNSNWRGPVWFPVNFLLIESLQKFHHFFGDSFTVEFPK
                     GSGRELTLWQVADELSRRLTGIFLAGPDGRRPAYGGTAVFQTDPHWKDLLLFHEYFNG
                     DDGAGLGASHQTGWTGLVAKLLQQAGDGRPRPRE"
     gene            3187..3561
                     /locus_tag="PLB01_14135"
     CDS             3187..3561
                     /locus_tag="PLB01_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78489.1"
                     /translation="MNRRQFAATLGAAVAGLVAGKTLLAEDKPADKPAKHACKGLNSC
                     KGQGGCATKENSCAGQNACKGKGGCATAAKHDCAGKNECKGQGGCKSGDNKCAGKNSC
                     KGKGGCASPMKNAKKKDAPKAS"
     gene            3575..4447
                     /locus_tag="PLB01_14140"
     CDS             3575..4447
                     /locus_tag="PLB01_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HQQ78490.1"
                     /translation="MGDRFGLPDLGVGVGLRTVHFGHILSKEPDVDFYEALTENFLDT
                     GGRPLHVLDRVAERTPVVLHGVSMSIGGTDPLDFDYLRKVKALAVRTRAHWVSDHLCF
                     TGVMGRNTHDLLPLPYTEETLKHVTRRAKTVSDFLERPLVLENPSSYVEFRASTIPEW
                     TFFDRLLRDADCGMLLDVNNVYVSSVNHGFDPVAYLDALPHDRVVQYHLAGHTDKGTY
                     LLDTHSARVKDEVWSLFRHANSLTGPRATLLEWDEDIPDFETVHAEARKALAHRPALA
                     RSASEDPVRSGARG"
     gene            4440..5240
                     /locus_tag="PLB01_14145"
     CDS             4440..5240
                     /locus_tag="PLB01_14145"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HQQ78491.1"
                     /translation="MADLRTLERWMQAAIVHPGTIEEAVRHRSATRHLPRREAERAIL
                     PSKTMAPLERLAVYQGMYPLRMHDALAADYPGLREFLGHHGFEHFVADYVAVHPSRSY
                     TLNRLGDHVPEFVRTWHHAKRAFLADLARLELAITEAFDAEEDAPAAPPVHVDADWER
                     RRFGIAPTLRLLAFRHAAGPALAALRAGRRASTRPKASWVAVHRRSFVVYRLDLTRGE
                     FHLLSALAAGQPLGKALRLAARKAGGALSPAAVRKAFRVWTSEGIIRN"
     gene            complement(5245..5478)
                     /locus_tag="PLB01_14150"
     CDS             complement(5245..5478)
                     /locus_tag="PLB01_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HQQ78492.1"
                     /translation="MSDVKITVRNNGSLLVSGPVTLEDAAGRPFGLSGRTAIGLCRCG
                     HSENKPFCDGSHSRAGFRSEVVACELPPPKPRP"
     gene            complement(5490..5990)
                     /locus_tag="PLB01_14155"
     CDS             complement(5490..5990)
                     /locus_tag="PLB01_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HQQ78493.1"
                     /translation="MTGTPVFRRPMGALVVAVAFLAAAGRAHSASPSLVKWRPIVAGE
                     AEAKKAGKLVLYYFTADWCGPCHLLDRQVFSQKAVADQIAKDYVPIIVRDVSRETGKN
                     APEMLKLADRYGLRGFPTLVVSRPGFDKGVTMEGWDGPALAIEFLKGARQRFLTAEKA
                     EKKKKT"
     gene            complement(6000..>7270)
                     /locus_tag="PLB01_14160"
     CDS             complement(6000..>7270)
                     /locus_tag="PLB01_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78494.1"
                     /translation="PLATWLALEVVRFGRPLASYGDQRFAHPFFDGAWRLLVGANKGL
                     LLYFPLALLALWALARALRRRTVRAEAVAVLVLLGAFVGVYARWWAWDGTGGWGPRFL
                     VPLVPLLAGAAGAAASSRAGRATGVALSVLGVGVNALGTFQTEASAFAYLWSTGFARV
                     SPELYAEYPKSFQPPPVENGVFRLWRHVPAASDSAFSALRVHPFLLGARLENLGDAER
                     RQRLARPPWLASHPDAVPDVPPPSPMITTRTPLEVYLTSPFRWPHLFMSFTRPRGERP
                     GTYNPAFYAGLADQALRNLDIGRPDRAARIAETLFALLPSGYTAALRLEGLRLSGQDD
                     AAYAFAEGLPDRVRRAPAVVLVQALRARDRGQDAFAAVLLAEAARGIRTRAMEDALRR
                     PPSEWPRSFRELSSEISDGSDAGAAAAPPR"
BASE COUNT         1113 a         2666 c         2478 g         1013 t
ORIGIN      
        1 ccgcccgaac gagctctcgg gcggccagcg ccagcgcgtc gcgttcgcgc gcgcgctggc
       61 cgtgaacccg cgcgtcctcc tcctcgacga gccgttcggc gcgctcgacg cgggcgtccg
      121 gctcgagctc ggcgagtggc tcctgaggct ccacgacgag accggcctga ccacgctcct
      181 cgtcacgcac gaccagcccg aggcgttcgc gctcgcgtcg cacctcgtcg tcctcttcga
      241 cgggaaggtc gcgcaggccg ggacgccggc cgagctgcgg gcgaattcgg ccgacgaccg
      301 cctggccgct ttcctgcgcg tcgtgcgccg ctcccccgcc gccggctgac ccgccgggtt
      361 acccttccgg cgtggaggcg tgatgccgaa gtccgggccg ctcgatccgg agtccctccg
      421 cctggaggag aaccgctccc gcaaggcgaa ctggaagcgg tggggtccgt acctcgccga
      481 gcgccagtgg ggcacggtgc gcgaggacta cggcgccgag ggccagccgt ggacgtattt
      541 cccgcacgac cacgcgcgct cccgcgcgta ccgctggggg gaggacggga tcgcgggctt
      601 ctccgaccgc cacggcctga tctgcctcgc gcccacgctc tggaacgggc gcgatccgat
      661 cctcaaggag cgcctcttcg gcctctccgg gcccgagggc aaccacggcg aagacgcgaa
      721 ggagtactgg ttcaccctcg acgcgacgcc cacgcacgcg tacatgcgga tgctctacaa
      781 gtacccgcag agggagttcc cgtaccgcgc gctcgtcgag gagaacgcgc gccggacccg
      841 gctggatccc gagttcgagc tcctcgacac gggcgtcttc gaggagagcc gctactggga
      901 cgtcttcgtc acgtacgcga aggcctctcc cgaggacctg ctcgtgacga tcgaggccgt
      961 aaaccgcggc cccgaagcgg ccccgctcca cgtccttccg acgatctggt tccggaacac
     1021 gtggtcctgg gggctcgacg cgcgcctgcc gcggctcgcg gcggcggcgc ccccggacgg
     1081 cgccgtggcg atcgcggcgc accacccgta ctacggcgac cggacgctct acgtggaagg
     1141 cgctccggac ctgctcttca ccgacaacat cacgaacgcg gcactcctct tcggcgccga
     1201 gaacgaaggc cgcttcgtca aggacgcctt tcacgcacgt gtcgtccagg gcgtcgagga
     1261 cgccgtgaac cccgccggct acgggacgaa ggcagccgcc tggttccggt tcgacgtccc
     1321 cgccggagag tcgcgcaagg tgcgcctgcg cctctcggac cgtgcgctcg ccgcgccgtt
     1381 cggcgaggac ttcgaccgga cggtcgcggc gcgccggagc gaggcggacg ccttctatgc
     1441 ccgcgtgatc cccccggagc tctccgcaga cgcgaagaac gtcatgcgtc agtcgctcgc
     1501 cgggatgctc tggtccaagc agtactacgg ctacgacgtg gcgcgctggc tcaagggcga
     1561 cccgggccag ccggcgcctc cgcccgggcg cgagcagggg cgcaacgcgg gctggaggca
     1621 cctcttcaac gcggacgtcc tctcgatgcc ggacaagtgg gagtacccct ggtacgccgc
     1681 gtgggacctc gccttccaca cgatcccgct cgcgctcgtg gacccggact tcgccaagga
     1741 ccagctcatc ctcctcctgc gcgagtggta catgcatccg aacgggcagc tccccgccta
     1801 cgagtggaag ttcgaggacg tcaacccgcc cgtccacgca tgggccgcgt tgcgcgtcta
     1861 caagatcgac gagaagcgtt ccggcacgcc cgaccgcgag tttctcgcac gcgtcttcca
     1921 caagctgctc ctgaacttca cctggtgggt gaaccgcaag gacgagaacg ggaacaacgt
     1981 cttccagggc ggcttcctcg gcctcgacaa catcgggctc ttcgaccgca gcaagccgct
     2041 gccgctcggc gcgacgctcg agcagtccga cgccacgagc tggatgggga tgtactgcct
     2101 gaacatgggc acgatcgcgc tcgagctcgc gcgcgcggac agggcctacg aggacatcgc
     2161 ctcgaagttc ttcgagcact tcatgtacat ctcgaaggcg atgaacgccc cggagcacgg
     2221 gagcgggctc tgggacgagg cggacgggtt cttctacgac gttctccgcc tcgccgacgg
     2281 ccacgggatc cccatgcgga tccagtccat ggtcggcctc atccccctct tcgccgtcgc
     2341 gacgctcgaa ccgtacgtcc tcgaggcgtt ccccggcttc cgccgccgga tgcagtggtt
     2401 cctcgaccac ggctcggcgt acggcgccaa ggtcgagacg caggcgacgg ccgccggaac
     2461 ccgccgcctc ctgtcgctcg tcggacggga gcgcctccgg cggatcctgg agccgctcct
     2521 ggatccggac ggcttcctgt ccgaccacgg agtccggtcg ctgtcccggg cccaccgcga
     2581 ccggccgttc gtggttccgt gggacggccg cgagctgcgc gtcgactacg agccggccga
     2641 gtcgcgcacg agcctcttcg gcggaaactc gaactggcgc ggccccgtct ggttcccggt
     2701 gaacttcctc ctgatcgagt cgctccagaa gttccaccat ttcttcggcg attccttcac
     2761 ggtggagttt ccgaagggct ccggacgcga actcaccctt tggcaggtcg ccgacgagct
     2821 ttcccgccgt ctgaccggga tcttcctcgc ggggcccgac ggccggcggc ccgcctacgg
     2881 cgggaccgcg gtgttccaga cggaccccca ctggaaggac cttctccttt tccacgagta
     2941 cttcaacggc gacgacggcg ccgggctcgg cgcctcccac cagaccggct ggacgggcct
     3001 cgtggcgaag ctcctccagc aggccgggga cggccggccc cgtccccggg aataaacggc
     3061 cctccgggag gcattcaggg ggagcccgcg cccgttcgtt tcgagaccga gtctccgatc
     3121 cagtcgcaat ctcagatccg tagtgatatg aagagaccgc aacccgagcg aataaaagga
     3181 ggcctcatga atcgtcgtca gttcgccgcc accctcggtg ccgccgtcgc cggcctcgtc
     3241 gccggcaaga ccctcctcgc ggaggacaag cctgccgaca agccggccaa gcacgcctgc
     3301 aagggcctga actcctgcaa gggccagggg ggctgcgcca cgaaggagaa cagctgcgcg
     3361 ggccagaacg cgtgcaaggg caagggcggc tgcgccaccg ccgccaagca cgactgcgcc
     3421 ggcaagaacg agtgcaaggg ccagggcggc tgcaagtccg gcgacaacaa gtgcgccggc
     3481 aagaactcgt gcaagggcaa gggcggctgc gccagcccga tgaagaacgc gaagaagaag
     3541 gacgccccca aggcgagctg atccggcgac gttcatgggc gaccgctttg gactgcccga
     3601 cctcggggtg ggggtcgggc tccgcaccgt ccacttcggg cacatccttt cgaaggagcc
     3661 cgacgtggat ttctacgaag ccctcaccga gaacttcctc gacacgggcg ggcgcccgct
     3721 ccacgtcctc gaccgggtcg cggagcggac gcccgtcgtc ctccatgggg tctcgatgtc
     3781 gatcggcggg acggatcccc tcgacttcga ctacctccgg aaggtcaagg cgctcgcggt
     3841 ccggacgcgg gctcactggg tctcggacca cctctgcttt accggcgtca tgggccgcaa
     3901 cacgcacgac ctgcttcccc tcccctacac cgaggagacc ctgaagcacg tcacgcggcg
     3961 ggcgaagacc gtatcggact tcctcgagcg gccgctcgtc ctcgagaacc cgtcctcgta
     4021 tgtcgagttc cgcgcgtcga cgatcccgga gtggacgttt ttcgaccggc tcctccgcga
     4081 cgcggactgc ggaatgctcc tcgacgtcaa caacgtctac gtgagctccg tgaaccacgg
     4141 gttcgacccc gtcgcgtacc tcgacgccct tccgcacgac cgcgtcgtcc agtaccacct
     4201 tgccggccac acggacaagg ggacgtatct cctcgacacg cacagcgcgc gcgtgaagga
     4261 cgaggtctgg agcctcttcc gccacgcgaa ctccctcacg ggcccgcgtg cgacgctcct
     4321 cgagtgggac gaggacatcc cggacttcga gaccgtccac gcggaggcga ggaaggcgct
     4381 cgcccaccgg cccgcgctcg cacggagcgc ctccgaagat cccgtccggt cgggcgcccg
     4441 tggctgatct ccgcacgctc gagcgctgga tgcaggccgc gatcgtgcac ccgggcacga
     4501 tcgaggaggc cgtgcgccac cgctccgcga cacgccacct tccacggcgc gaggccgagc
     4561 gcgccatcct gccctcgaag acgatggcgc ccctcgagcg cctcgccgtc taccagggca
     4621 tgtacccgct gcggatgcac gacgcgctcg cggccgacta cccgggcctg cgggagttcc
     4681 tcgggcacca cgggttcgag catttcgtgg ccgactacgt ggcggtccac ccgtcccgga
     4741 gctacaccct caaccgactg ggcgaccacg tcccggagtt cgtgaggacg tggcaccacg
     4801 ccaagcgcgc gttcctcgcc gacctcgcgc gtctcgagct cgcgatcacc gaggccttcg
     4861 acgcggagga agacgccccc gcggcgcctc ccgtgcacgt ggacgccgac tgggagcgcc
     4921 gccggttcgg catcgcgccg acgctgcggc tcctggcctt ccgccacgcc gccgggcccg
     4981 cgctcgccgc cctcagggcg ggccgaaggg cctcgacgcg cccgaaggcg tcatgggtcg
     5041 ccgtccaccg ccgaagcttc gtcgtctacc ggctggacct gacccgcggg gagttccacc
     5101 tgctgtctgc gctcgccgcc ggccagccgc tcgggaaggc tctgcgtctc gcggcccgca
     5161 aggccggcgg ggcgctgtcc cccgccgcgg tccgcaaagc gtttcgcgtc tggacgtccg
     5221 aagggatcat ccggaactga aggctcaggg cctcggcttc ggcggcggca gctcgcaggc
     5281 cacgacctcg gatctgaacc ccgcgcggga gtgcgagccg tcgcagaagg gcttgttctc
     5341 cgagtggccg cagcggcaga gcccgatcgc cgtgcggccc gaaagcccga acggccgtcc
     5401 ggcggcgtcc tcgagcgtga cgggtccgct cacgagcagc gagccgttgt tgcggacggt
     5461 gatcttgacg tcgctcacgg ggcctccgat caggtcttct tcttcttttc ggctttctcg
     5521 gccgtcagaa atctctggcg cgcgcccttg aggaactcga tcgccagcgc cgggccgtcc
     5581 cagccctcca tcgtgacgcc cttgtcgaac ccggggcgcg acacgacgag agtcggaaag
     5641 ccgcgcagcc cgtatcggtc ggcgagcttc agcatctcgg gcgcgttctt tcccgtctcg
     5701 cgggacacgt cccgcacgat gatcggaacg tagtccttcg cgatctggtc ggcgaccgcc
     5761 ttctgcgaga agacctgccg atcgaggagg tggcagggcc cgcaccagtc ggccgtgaag
     5821 tagtagagga cgagctttcc cgccttcttc gcctcggcct cccccgcgac gatggggcgc
     5881 cacttgacga gactcggcga cgccgagtgc gcgcggccgg cggccgccag gaacgccact
     5941 gcgacgacga gcgcgcccat cgggcgccgg aaaacggggg ttcctgtcat tcccggatgt
     6001 tagcgcggcg gcgcggcggc ggcgcccgca tccgacccgt ccgagatctc ggagctgagc
     6061 tcccggaagc tcctcggcca ttcggacggc ggccggcgca gggcgtcctc catcgcgcgc
     6121 gtccggatgc cgcgggctgc ctcggcgagg agaaccgcgg cgaatgcgtc ctggccgcga
     6181 tcgcgcgcgc ggagggcctg cacgagcacg accgcggggg cgcgccggac gcggtccggg
     6241 agcccctcgg cgaaggcgta cgcggcatcg tcctgtcccg acaggcgcag gccctcgaga
     6301 cggagggcgg cggtgtatcc cgacggcagg agcgcgaaga gcgtctccgc gatccgggcc
     6361 gcacggtccg gccggccgat gtcgaggttc cggagcgcct ggtccgcaag gcccgcgtag
     6421 aaggccgggt tgtacgttcc cggccgctct ccgcggggcc gcgtgaagct catgaagagg
     6481 tgcggccagc ggaacggcga ggtcaggtag acctcgagcg gcgtccgcgt cgtgatcatc
     6541 ggcgacggcg gcggtacgtc gggaacggcg tccgggtgcg aagccagcca cggcgggcgc
     6601 gcgagcctct gccggcgctc cgcgtcgccg aggttctcga gccgcgcccc gaggagaaac
     6661 ggatggacgc gcagggccga gaacgccgag tcggacgccg cgggcacgtg gcgccagaga
     6721 cggaagacgc cgttctcgac gggaggcggc tggaaggatt tcgggtactc ggcgtagagc
     6781 tcgggcgaga cgcgcgcgaa gcccgtggac cagaggtacg cgaacgccga ggcctcggtc
     6841 tggaacgtcc cgagcgcgtt cacgccgacg ccgaggacgg agagcgcgac gcccgtggcc
     6901 ctcccggctc gcgaggacgc cgccgcgccc gcagcgcctg ccaggagggg gacgagcggg
     6961 acgaggaagc gcggccccca tcctcccgtc ccgtcccacg cccaccagcg cgcgtacacg
     7021 cccacgaacg ccccgaggag gacgaggacg gcgacggcct ccgcgcgcac ggtgcgtcgg
     7081 cgcagcgcgc gtgcgagcgc ccagagggcg agaagcgcga gcgggaaata gagcagcagc
     7141 cccttgttcg ctcccacgag caggcgccac gcgccgtcga agaacgggtg cgcgaacctc
     7201 tggtcgccgt acgaggccag cgggcggccg aaacgcacga cctccagcgc gagccaggtc
     7261 gcgagcggtc
//
LOCUS       DAOMFY010000199         7217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3310_length_7217_cov_8.655404, whole genome shotgun sequence.
ACCESSION   DAOMFY010000199 DAOMFY010000000
VERSION     DAOMFY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_7217_cov_8.655404"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="rpe"
                     /locus_tag="PLB01_14165"
     CDS             complement(<1..601)
                     /gene="rpe"
                     /locus_tag="PLB01_14165"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQQ78495.1"
                     /translation="MSAGKVALAPSILSADFGRLAEAVAACEAGGADWIHFDVMDGHF
                     VPNLTVGPIVLKALGKATKVPFDVHLMIEEPFRTLDQYLDAGARRVAVHVEAEPNLHR
                     FAQHVRGRGVSPGVAINPGTSLDSLDEAVRFVDFVLVMSVNPGWGGQSFIPGSLERVA
                     RLSASIRKAGSAATIAVDGGVGEANAGALAAAGASHLVAG"
     gene            complement(598..1323)
                     /locus_tag="PLB01_14170"
     CDS             complement(598..1323)
                     /locus_tag="PLB01_14170"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQQ78496.1"
                     /translation="MGGVFGRVLYALVLAGVLATATWVSFTRFVAGKSLKTPSFVSLT
                     PDEAAARAAELGLSVQVDAAQDAFDDAVPAHRVRAQVPAADTAVKSGQTIRLFLSLGP
                     RAVRMPDLAGLPARAATLALSKAGLKEAAVASARLPGPAGVVAQGVSAGTIAGPDTPV
                     DILVNRGPSDVAWVMPDLIGRDFERVRTAFEERGFRIGGVRSQAYEGAATGTILRQFP
                     LSGAPVTRRDALSFVIASPEAAP"
     gene            1390..2367
                     /locus_tag="PLB01_14175"
     CDS             1390..2367
                     /locus_tag="PLB01_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78497.1"
                     /translation="MAEPAAPDPGTPALPFSRADLHLHTNASIFKYFRAGNTRDSYND
                     PDETYRLAKARGMDFVTFTDHDTVDGCLKFQDRHPDYADFFVSAEIETFFPRTGHRIH
                     VNVFDLTLAQWKVINVKRRNIFDLVEYLRSENLLYSANHLFQSYRMRQAPEDFFTKML
                     ELFDVFEVKNGSMAYQHNALVEDLLTAAKEKRGSLALIGGSDAHTYPPVASVFTMAPG
                     ATWREFLGYVREGECLSWGTEMGFTNVLTDVYKLLGKYYTTVVDFKNPEFTAMQKARH
                     FLIALASVPFNAAGVPAAVYSLNYAKQLALSRRLKTHFDRKGFSGDSGR"
     gene            complement(2364..3593)
                     /gene="ispG"
                     /locus_tag="PLB01_14180"
                     /gene_synonym="gcpE"
     CDS             complement(2364..3593)
                     /gene="ispG"
                     /locus_tag="PLB01_14180"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQQ78498.1"
                     /translation="MPRHRTVGVPVGGIQVGGGAPVVVQSMSNTDTADVDATVRQAVE
                     LAEAGSELVRITVNVDEAARAVPEIKRRLLAAGCGVPLIGDFHYNGHVLLTKYPEMAK
                     ALDKYRINPGNVGTGKRRDEQFATICGVARDLGRPVRIGVNGGSLNQELVMQKMQENT
                     DRDLGKTSDEIVAECMVLSALQSTELALECGLKKDQIIISCKVSRPNELVSVYRDLAA
                     KTDQPLHLGLTEAGMGTKGLVWSSAAMGILLAEGIGDTIRVSLTPRPGGDRREEVYAA
                     CELLQALGLRSFAPSVTACPGCGRTTSTTFQELAERIQGYVRERMPEWKARHEGVETM
                     TLAVMGCIVNGPGESKAANIGISLPGTGEEPSCPVYVDGQKFATLKGNYDELSAAFRK
                     LVDDYVETKYPRRDGSA"
     gene            complement(3593..4927)
                     /gene="rseP"
                     /locus_tag="PLB01_14185"
     CDS             complement(3593..4927)
                     /gene="rseP"
                     /locus_tag="PLB01_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQQ78499.1"
                     /translation="MNIPTNVLALVFVLSFLIVFHEGGHFAVAKFFKFPVEIFSLGFG
                     KRLFGWKRKETDYRVSLIPLGGYVKVVGLGPDESDIVAGEHAAPAQPGTRLQRLLILF
                     AGPGVNLVLAFLLTALAFLVGVEVAAYKSETPVVKVVDRGSPAEKAGIREGDRVDTIS
                     GKPVKTWGDVEIETGLAPREKLAVGIVRDGAPLTLELTPEPRTKYDIGYTGLNPAILA
                     VVARVVPGYPGAKAGLRVGDRIVSVNGKPVVLYFEVVRFIREAASGFDKDGAKPIEMV
                     VTRGSETITLNVVPVKEGENWRIGFAPKEETTVRRLGLGAALAASWRENVRQVKVTGQ
                     IVGRLFRGTGSMRQLSGPIDIAKFSGEAARSGVAPLVMLMGVLSLQLGLLNLLPIPLL
                     DGGQIFVTLLEAVARRDFSIKVKERLLQAGFVFLVLIMVTVLYFDVIKNVTF"
     gene            complement(4943..6169)
                     /locus_tag="PLB01_14190"
     CDS             complement(4943..6169)
                     /locus_tag="PLB01_14190"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQQ78500.1"
                     /translation="MTPKKRIALLGATGSIGDSALSVVAHFPERFEIVSMSAGSNVEK
                     LLPAIAAARPRVVSVKTKEDAARVRSEFPGVSVGFGEQGLVDVATHPDADVVLGGIVG
                     SAGLVPAYEAVKLGKTLALANKETLVVAGEPLMRAARAAGAAVLPVDSEHCALHQALR
                     AGAASEVRRLVLTASGGPFRMRPFETFSAISVEDALRHPTWKMGPKITIDSATMMNKG
                     LEVIEARWLFDVEVERIGVVIHPQSIVHSMVEYVDGSVLAQMSPNDMRFPILYALCYP
                     ERIDVYSARADRALDTPLPRLDLAALGTLTFEALDEARYPAVPLACAALRTGGSAPAV
                     LNAANEVAVEAFLAGRIPYLGIVETVARVLARHKVLPISSVAAALEADAWARREAETL
                     LPALERPAATNRAVPA"
     gene            complement(6166..6984)
                     /locus_tag="PLB01_14195"
     CDS             complement(6166..6984)
                     /locus_tag="PLB01_14195"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQQ78501.1"
                     /translation="MRFQREITAAVLIPAVLAVLVFAPTWAFSLLVAAAASVALLEFY
                     ALLAAGGWYVPRAAGLVLFAGLLAAAHFAAPGVLLALAAATLIVLPTLVMFASPATAL
                     LLPSSAGAVFATLYVALGGASIIGLRAHGWQPVVFLFAVVWAGDSAAYYVGRRWGTRK
                     LAPVVSPKKTWEGLWGQLLGGTLFGAAAAFLIAPGARGTASAALAGAAFGLVVSATAV
                     LGDLIESTFKRSVAVKDSGGLLPGHGGLLDRLDSLLYASPVLLLALTLLPGGPR"
     gene            complement(6981..>7217)
                     /locus_tag="PLB01_14200"
     CDS             complement(6981..>7217)
                     /locus_tag="PLB01_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HQQ78502.1"
                     /translation="PDPDLLIRTSGEMRISNYLLWQIAYAEIYVTPVLWPDFRARHFL
                     EALAEFQKRERRYGGVTENEPLREDGPRPAAATR"
BASE COUNT         1142 a         2548 c         2539 g          988 t
ORIGIN      
        1 cccccgccac gaggtgggac gccccggcgg ccgcgagggc gcctgcgttg gcctccccga
       61 ccccgccgtc cacggcgatc gtcgccgccg aacccgcctt ccggatcgac gcggagaggc
      121 gggcgacgcg ctcgagggag cccggaatga acgactggcc gccccacccg gggttcaccg
      181 acatcacgag gacgaagtcc acgaaacgca cggcctcgtc gagggagtcg agcgaggtcc
      241 cggggttgat cgcgaccccc ggcgacacgc cgcggcccct cacgtgctgt gcgaaccggt
      301 gcaggttcgg ctcggcctcg acgtgaaccg cgacgcgccg cgcgccggcg tcgaggtact
      361 ggtcgagcgt ccggaacggc tcctcgatca tgagatgaac gtcgaacggg accttcgtcg
      421 ccttcccgag ggctttgagg acgatgggcc cgaccgtgag attcgggacg aaatgcccgt
      481 ccatgacgtc gaagtggatc cagtcggccc ctccggcctc gcacgcggcg acggcttccg
      541 cgaggcggcc gaagtccgcc gagagaatcg acggggcgag cgcgaccttc ccggcgctca
      601 cggcgccgcc tccggcgagg cgatgacgaa gctcagcgcg tcgcgccgcg tcacgggcgc
      661 cccggagagc gggaactggc ggaggatcgt gccggtcgcc gcgccctcgt acgcctggct
      721 cctcaccccg ccgatgcgga agccgcgctc ctcgaaggcc gtcctcacgc gctcgaagtc
      781 gcgcccgatg aggtcgggca tcacccacgc gacgtcggag ggcccgcggt tcacgaggat
      841 gtcgacgggg gtgtccgggc ccgcgatcgt gccggccgac acgccctgcg cgacgacgcc
      901 cgccggtccg ggaaggcgcg ccgaggcgac ggcggcctcc ttcaggccgg ccttcgacag
      961 ggcaagcgtc gcggcgcgtg ccgggaggcc tgcgaggtcg ggcatgcgca cggcgcgcgg
     1021 cccgagagag agaaagaggc ggatcgtctg gccgctcttg accgccgtgt cggccgcagg
     1081 aacctgcgcg cggacccggt gggcgggcac tgcgtcgtcg aaagcgtcct gcgccgcgtc
     1141 cacctggacc gagagcccga gctcggcggc gcgagccgca gcctcgtcgg gcgtgaggct
     1201 cacgaagctc ggggtcttga gggacttgcc cgcgacgaag cgcgtgaacg agacccaggt
     1261 tgcggtcgcg aggacaccgg ccagcacaag cgcgtacagc actctcccga agacccctcc
     1321 catggcggca ctctagcagg agcacggctc cttgactcgc cccggacgga cgcggagact
     1381 cgtttcccca tggccgaacc cgccgccccg gatccgggca ctcccgcgct ccccttctcc
     1441 cgggcggacc tccacctgca cacgaacgcg tcgatcttca agtacttccg cgcgggcaac
     1501 acacgcgact cctacaacga cccggacgag acgtaccgcc tcgcgaaggc ccgcgggatg
     1561 gacttcgtga ctttcacgga ccatgacacc gtggacggct gcctgaagtt ccaggaccgc
     1621 cacccggact acgcggactt cttcgtctcg gccgagatcg agacgttctt ccccaggacg
     1681 ggccaccgca tccacgtcaa cgtcttcgac ctcacgctcg cgcagtggaa ggtcatcaac
     1741 gtcaagaggc ggaacatctt cgacctcgtc gagtacctcc gttcggagaa cctcctctac
     1801 tcggcgaacc acctcttcca gtcctaccgg atgcggcagg cccccgagga cttcttcacg
     1861 aagatgctcg agctcttcga cgtcttcgag gtgaagaacg gctcgatggc ctaccagcac
     1921 aacgcgctcg tcgaggacct cctgacggcc gccaaggaaa agcgcggctc cctcgcgctc
     1981 atcggcgggt ccgacgcgca cacgtacccg ccggtcgcga gcgtcttcac gatggcgccg
     2041 ggcgccacgt ggcgggagtt cctcggatac gtccgggagg gcgagtgcct gtcgtggggg
     2101 accgagatgg gcttcacgaa cgtcctcacg gacgtctaca agctcctcgg gaagtactac
     2161 acgaccgtcg tggacttcaa gaatcccgag ttcacggcga tgcagaaggc gcggcacttc
     2221 ctgatcgcgc ttgcgtccgt cccgttcaac gcggccggcg ttcccgcggc ggtctattcg
     2281 ctgaactacg ccaagcagct cgcgctttcg cggaggctca agacgcactt cgaccgcaag
     2341 ggcttctccg gggactcggg ccgctaggcg gacccgtccc ggcgcgggta cttcgtctcg
     2401 acgtagtcgt cgacgagctt gcggaacgcg gccgagagct cgtcgtagtt gcccttgagc
     2461 gtcgcgaact tctgtccgtc gacgtaaacg gggcagctcg gctcctcgcc cgtgccgggc
     2521 agcgagatcc cgatgttcgc ggccttcgac tcgcccggcc cgttcacgat gcagcccatg
     2581 accgcgagcg tcatcgtctc gacgccctcg tgccgcgcct tccactcggg catccgctcg
     2641 cgcacgtatc cctggatgcg ctcggcgagc tcctggaacg tcgtcgaggt cgtgcgcccg
     2701 cagcccgggc acgccgtgac ggacggcgcg aagctgcgca acccgagcgc ctgcaggagc
     2761 tcgcacgcgg cgtagacctc ctcgcgccgg tcgccgccgg ggcgcggcgt gagcgacacg
     2821 cgaatcgtgt cgccgatgcc ctcggcgagg aggatcccca tcgccgccga cgaccacacg
     2881 aggcccttcg tccccatccc ggcttccgtg aggccgagat gcagcggctg gtccgtcttc
     2941 gcggcgaggt cgcggtagac cgagacgagc tcgttcgggc gcgagacctt gcacgagatg
     3001 atgatctggt ccttcttcag gccgcactcg agcgcgagct cggtggactg gagcgcggag
     3061 aggaccatgc actccgcgac gatctcgtcg gaggtcttcc cgaggtccct gtccgtgttc
     3121 tcctgcatct tctgcatgac gagctcctgg ttcagggagc cgccgttgac cccgatgcgc
     3181 acgggccgcc ccaggtcccg cgcgacgccg cagatcgtcg cgaactgctc gtcgcggcgc
     3241 ttgcccgtcc cgacgttgcc cgggttgatc cggtacttgt cgagggcctt cgccatctcc
     3301 gggtacttcg tcaggagcac gtgcccgttg tagtggaagt ccccgatgag cggcacgccg
     3361 cagccggcgg cgaggagccg ccgcttgatc tccgggacgg cccgcgcggc ctcgtccacg
     3421 ttcacggtga ttcgcacgag ctcggaaccc gcctcggcaa gctcgacggc ctgccggacc
     3481 gtcgcgtcca cgtccgccgt gtccgtgttc gacatggact ggacgacgac gggggcgccg
     3541 ccgccgacct ggatccctcc gacgggcacg ccgaccgtgc gatgacgcgg catcagaacg
     3601 tgacgttctt gatgacgtcg aagtagagga cggtgaccat gatgagcacg aggaacacga
     3661 agcccgcctg caggaggcgc tccttgacct tgatcgagaa gtcccgccgc gcgacggcct
     3721 cgaggagcgt gacgaagatc tggccgccgt cgaggagcgg gatcgggagg aggttcagga
     3781 ggccgagctg cagcgagagg acgcccatga gcatgacgag cggggccacg cccgagcgcg
     3841 ccgcctcgcc ggagaacttc gcgatgtcga tcgggcccga gagctggcgc atggagcccg
     3901 ttccgcggaa gagacggccg acgatctggc cggtcacctt cacctgccgg acgttctcgc
     3961 gccacgaggc cgccagcgcg gcgccgagcc cgaggcgccg gaccgtcgtc tcctccttcg
     4021 gggcgaagcc gatgcgccag ttctcccctt ccttgacggg gacgacgttg agcgtgatcg
     4081 tctcgcttcc gcgcgtgacg accatctcga tgggcttcgc gccgtccttg tcgaacccgg
     4141 aggccgcttc gcggatgaac cgcacgacct cgaagtacag gacgacgggc tttccgttca
     4201 cggagacgat ccggtcgccg accctgagac cggccttcgc gccgggatag cccgggacga
     4261 cgcgggcgac gaccgcgagg atcgcgggat tgaggcccgt gtacccgatg tcgtacttcg
     4321 tccgcggctc gggcgtgagc tcgagcgtga gcggcgcgcc gtcgcgcacg atcccgacgg
     4381 ccagcttctc gcgcggggcg aggccggtct cgatctcgac atccccccac gtcttcacgg
     4441 gcttgcccga gatcgtgtcg acgcggtcgc cttcccggat gcccgccttc tcggccgggc
     4501 ttccgcggtc gacgaccttc accacgggcg tctcggactt gtacgccgcg acttcgacgc
     4561 ccacgaggaa cgcgagcgcc gtgagcagga acgcgagcac gaggttcacg ccgggcccgg
     4621 cgaagaggat gaggaggcgc tgcaggcgcg tgccgggctg cgcgggggcc gcgtgctcac
     4681 cggccacgat gtcgctctcg tcgggcccga ggccgacgac cttcacgtag ccgccgagcg
     4741 ggatgagcga gacgcggtag tccgtctcct tccgcttcca gccgaagagc cgcttgccga
     4801 agccgaggga gaagatctcg accgggaact tgaagaactt cgccacggcg aagtgcccgc
     4861 cctcgtgaaa gacgatcagg aacgagagca cgaagacgag ggcgaggacg ttggtcggga
     4921 tgttcatgaa acctcgggtt gattacgccg gaacggcgcg gttggttgcc gccggccgct
     4981 cgagcgccgg gaggagggtc tcggcttcgc gccgcgccca ggcgtcggcc tcgagggccg
     5041 ccgcgacgga ggaaatcgga aggaccttgt gccgggcgag aacccgcgcg accgtctcga
     5101 cgatcccgag gtacgggatg cgtcccgcga ggaacgcctc gacggccacc tcgttcgccg
     5161 cgttgagcac ggccggggcc gagccgcccg tccggagcgc cgcgcacgcg agcgggacgg
     5221 cggggtaacg cgcctcgtcg agcgcttcga aagtgagcgt cccgagggcc gcgaggtcga
     5281 ggcgcgggag cggcgtgtcg agcgcgcggt ccgcgcgagc ggaatacacg tcgatcctct
     5341 ccggatagca gagcgcgtag aggatcggga agcgcatgtc gttcggcgac atctgcgcga
     5401 ggacggagcc gtccacgtac tcgaccatcg agtggacgat cgactgcggg tggatcacga
     5461 cgccgatgcg ctcgacctcg acgtcgaaca gccagcgcgc ctcgatgacc tcgaggccct
     5521 tgttcatcat cgtcgccgag tcgatcgtga tcttcgggcc catcttccac gtcgggtgcc
     5581 gaagggcgtc ttcgaccgag atcgccgaga acgtctcgaa cggccgcatc cggaacgggc
     5641 cgcccgaggc cgtgaggacg aggcgcctca cctcggaggc ggcgccggcg cgaagggcct
     5701 ggtggagcgc gcagtgctcc gagtccacgg ggaggacggc ggcgccggcc gcgcgcgccg
     5761 cgcgcatgag cggctcgccc gcgaccacga gcgtctcctt gttcgcgagc gcgagcgtct
     5821 tgccgagctt gacggcctcg taggcgggaa cgagccctgc cgatcccacg atccctccga
     5881 gcacgacgtc ggcgtccggg tgcgtggcga cgtccacgag gccctgctcg ccgaagccga
     5941 cggagacgcc cgggaattcg ctccgcacgc gcgccgcgtc ttccttcgtc ttcaccgaga
     6001 cgacgcgtgg ccgcgcggcc gcgatcgcgg ggaggagttt ctcgacgttc gagcccgccg
     6061 acatcgagac gatctcgaag cgctccggga agtgcgcgac gaccgagagg gccgagtcgc
     6121 cgatcgagcc cgtggccccg aggagcgcga tgcgcttctt cggggtcaac gcgggcctcc
     6181 gggcaggagg gtcagggcga ggagcagcac gggcgaagcg tagagcaagg agtccagccg
     6241 gtcgaggagg ccgccgtggc ccggcaggag gccgccggaa tccttgacgg cgacgctccg
     6301 cttgaacgtc gattcgatga ggtcgccgag gacggccgtg gccgacacga cgaggccgaa
     6361 ggcggctccc gcgagggcgg ccgacgcggt gccccgcgcg cccggggcga tcaggaacgc
     6421 cgcggccgcc ccgaagagcg tcccgcccag gagctggccc cagaggccct cccacgtctt
     6481 cttcgggctc acgaccggag cgagcttccg cgtcccccac cggcggccca cgtagtacgc
     6541 cgcggagtcg ccggcccaga cgaccgcgaa gaggaagacc acgggctgcc agccgtgcgc
     6601 gcgcaagccg atgatcgacg ccccgccgag ggcgacgtag agcgtcgcga agacggcgcc
     6661 cgccgaggac ggcaggagga gcgccgtcgc cggcgacgcg aacatcacga gcgtcggcag
     6721 gacgatgagc gtcgcggccg cgagcgcgag caggacgccg ggcgccgcga agtgcgcggc
     6781 cgcgaggagc cccgcgaaga gaacgagccc cgcggcgcgc ggcacgtacc agcccccggc
     6841 cgcgaggagc gcgtagaact ccagaagcgc gacggacgcg gccgccgcga cgaggagcga
     6901 gaacgcccac gtcggcgcga agaccagcac cgcgaggacc gccgggatga gaacggcggc
     6961 cgtgatctcc ctctggaacc tcaccgcgtg gccgccgccg ggcgcgggcc gtcctcgcgc
     7021 aggggctcgt tctccgtgac gccgccgtac cggcgctcgc gcttctggaa ctcggccagc
     7081 gcctcgagaa agtggcgggc ccggaaatcg ggccacagga ccggcgtcac gtagatctcc
     7141 gcgtacgcga tctgccagag gaggtagttc gagatccgca tctcgccgct cgtccggatc
     7201 aggaggtccg ggtcggg
//
LOCUS       DAOMFY010000200         7212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3314_length_7212_cov_8.929719, whole genome shotgun sequence.
ACCESSION   DAOMFY010000200 DAOMFY010000000
VERSION     DAOMFY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3314_length_7212_cov_8.929719"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLB01_14205"
     CDS             complement(<1..410)
                     /locus_tag="PLB01_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78503.1"
                     /translation="MDPLATARAFLPAWIPLVPLLALPFLFIGGWLVRSRTRFAAVMV
                     SSVAGALVAQVAGPLSVVGPGVAGFLGSAAAFAGYELGRRRAPPPPPGSIAGRIALVA
                     GSVLVIAAFQVGRAAAEPAISRRSPLAAVALTGGG"
     gene            463..1320
                     /locus_tag="PLB01_14210"
     CDS             463..1320
                     /locus_tag="PLB01_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HQQ78504.1"
                     /translation="MKLAVDAGVPAVTFLIMTIVGLDLTRADFERVRSRPRVLAAGLL
                     GPLVLLPPVALAVLAAVPMPDETRAGLLLLAVCPVGGISNTYNYLARASTALSVTLTA
                     LSCLLAVVTMPLLSSVFEVVLGRPFGFRAPVGPLAAQILVMLVLPVAVGMTIRSRAPD
                     FALRHEKAFRRAASVSIVLLLGFIVWSEWSRFVANFAVSAGAAGLFVLLAMAAGGLAA
                     VAAGADRRERFTLSVEFATRNAAIAAAVAVTLLGDTRFAVFATTYFVTEAVPILAAVA
                     LFRVRSRAA"
     gene            1317..2246
                     /locus_tag="PLB01_14215"
     CDS             1317..2246
                     /locus_tag="PLB01_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQQ78505.1"
                     /translation="MSKLQSGLGRLLAVGLLLGAASARAQDLEPRAYAPSPKDANFVI
                     VNYSYQSGEVLFDPSLPFSDVRAFINGVVGGYGHTFSLFGRISSVAAVVPYVWASVDG
                     NVGETYQAITRSGLGDPRLRFVVNLIGGPALPMKEFMARKPEPTLGFSLVAVVPTGQY
                     SNEKLINIGTNRWAFRPEFGLSFPVGKWTLEGAAGVWLFLDNDHAYPAENVRSQDPLY
                     SFQVHVGYTVRPGLWVAADATYYAGGKTYTNDVPGDTRQANTRAGLTASVPVARGHSV
                     KLSASRGVDSRIGSRFNAYGLAYQYLWLSRTGK"
     gene            2373..4088
                     /locus_tag="PLB01_14220"
     CDS             2373..4088
                     /locus_tag="PLB01_14220"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQQ78506.1"
                     /translation="MTSSTRASWTGVAAAAMSLAAALACTRGPAPEQTADAIYAGGPI
                     VTVNDAQPTAEVLAVKDGKILAVGTKAGIEKVHKGAATKAVDLAGKTLAPGFVDGHAH
                     FLGFGSQAVGANLLAPPDGTVNTVDDVVAKLQEFAKGPDVGRTGWIFGLGYDDALLGR
                     HPTRDDLDKVSKDVPVIAVHISGHFSAMNSAGLAKVGYTAATKDPEGGVIRRRPGSKE
                     PNGVLEELASIPKMIPAINATKPEDKDYFLKKGVELAKSFGYTTANEGRLMDFQQADL
                     VSAAGRGLLDIDVMSYADYTAKDVLPLISRTYKGRYRVAGVKITLDGSPQGRTAWRTI
                     PYLLPPDGQKKGYKGYPAIPDTKVVAAVIDEAYQKGWPVHVHGNGDAAIDQMIEAIRP
                     AQQKYGPADRRATLIHGQFLRKDQIPALKELQVFPSLFPMHTFYWGDWYDQIIGPELA
                     QQISPMRSVLNAGMIATSHTDAPVALPNLMQVMWATVNRTSRSGKVMGPGERLTPIEA
                     LKAITLWGAHEHFEEKAKGSLEAGKLADMVILSNNPLTIDPAKINTIQVMETIKEGKT
                     VYARK"
     gene            4118..6082
                     /locus_tag="PLB01_14225"
     CDS             4118..6082
                     /locus_tag="PLB01_14225"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQQ78507.1"
                     /translation="MRANRRNALLGASAATAVAVTLAASALAEEASLQDLAKAVQGLP
                     QVVIYTAKEIVTLDPAKPTAQAVAVVGGRILGTGSLEDVKAAVGKQPYTVNTTFANQV
                     IVPGFIAQHDHPLLAALTMTSTIIAIEDWVLPQGTSKAAKNRGEYLKRLAEANAAVKD
                     PNEPLVAWGYHQYFHGELKKADLDAISATRPILVWHRSAHEFYLNTAAEKKYGVTKEW
                     FEPLSASVKKQSDFPNAHYWEQGAFAVMPKIASAIATPEKLRAGLEFVKSYYHTNGVT
                     LGCEPGGLASKSLQDAQNAVLSDSSSPFRFYFLADGKSITATVPDEKVAEETGKLLSW
                     GQGMTAYLPKQVKLFADGAIFSQAMQVTRGYTDGHKGEWMMDLDFFARTFRVYWDLGY
                     QLHVHVNGDAGLDMVLDQLEANLRRNPRNDHRTVIIHFAVSRPEQVARIKRLGAIVSG
                     NPYYPVSLADNYRGNGLDPERADAMVRMGDVEKAGVSFSFHSDMPMAPGQPLLLMWSG
                     VNRITNDGNVRGPEQRVSRLGALKAVTLDAAYSLQMEREVGSIVSGKLANFTILAENP
                     LTVDPAKIKDIAVWGTVSEGRVLPVKRAAAAGPKAALGPVPDAPALAAAIEADRHDHE
                     NGAHGDACTAARGLASAVAMEASNPSRPAM"
     gene            complement(6096..>7212)
                     /locus_tag="PLB01_14230"
     CDS             complement(6096..>7212)
                     /locus_tag="PLB01_14230"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HQQ78508.1"
                     /translation="PGPEPRTATAGGGRWHVYFGDPHSHSILSDAKTGHPDQLLALSR
                     DRLGLDFDVVSDHSEMGLLQPSEFAELRATAGAFDAPGRFVSLTGWEWTAGSSFGHRV
                     LLYKDDGPEHTHRSTDRDSDTIEKLYRHAREHGAVMSPHHTGHALWGRWNPAAPHDEE
                     LEPNFEIASWHGRYEFYGNPREGRRQVPGHQYQDALRLGRHVGVMGASDTHHLSPGEG
                     GLTAVLAERLDRASLFDAIRNRRNYATTGARIVLEFTADGAPMGSILAGKGTMTLAVR
                     VEGTAPIDRIEIVRNLRDTFAAVRMRQAPGEAAAEYVLYDPKDPQGVNWLKTEDARRL
                     SFSVQDSPDAAGETSWYVRVTQTDGEQAWSSPVWARP"
BASE COUNT         1135 a         2570 c         2472 g         1035 t
ORIGIN      
        1 ccgccgcccg tcagcgccac ggcggccagg gggctccggc gggagatcgc gggctcggcg
       61 gccgcgcggc cgacctggaa ggcggcgatc acgaggacgg acccggcgac cagtgcgatc
      121 cggccggcga tgctccccgg gggcggcggg ggagcccgtc ttctcccgag ttcgtagccc
      181 gcgaacgcag ccgcgcttcc gagaaagccg gcgacgcccg gcccgacgac cgagagcggc
      241 ccggcgacct gcgccaccag cgcgccggcc accgacgaga ccatcacggc ggcgaagcgc
      301 gtccggctgc ggacgagcca acccccaata aagaggaagg ggagcgcgag gagggggacg
      361 agcggaatcc aggccggcag gaaggcgcgt gccgttgcga gcggatccac gcggggaggt
      421 tagcgcggag gcggggaggt tcgactacca tcggtcgaaa ccatgaagct cgccgtcgac
      481 gccggggtcc cggccgtgac gttcctcatc atgacgatcg tcgggctgga tctcacccgg
      541 gccgacttcg agcgcgtgcg gtcccggccg cgggtcctcg cggccggcct cctcgggccg
      601 ctcgtccttc tgccgcccgt ggccctcgcc gtcctcgccg ccgtcccgat gcccgacgag
      661 acgcgcgcgg gcctgctcct cctcgcggtg tgcccggtcg gggggatctc gaacacctac
      721 aactacctcg cgcgcgcgtc gacggccctc tccgtgacgc tcacggccct ctcgtgtctt
      781 ctcgcggtcg tgacgatgcc gctcctctcg agcgtgttcg aggtcgtcct gggccgcccg
      841 ttcgggttcc gcgcgcccgt gggcccgctc gcggcgcaga tcctcgtgat gctcgtcctg
      901 ccggtcgccg tcggcatgac gatccggtcc cgggcgcccg acttcgcgtt gcggcacgag
      961 aaggccttcc ggcgcgccgc gtccgtttcc atcgtcctcc tcctcggctt catcgtctgg
     1021 agcgagtggt cccggttcgt ggcgaacttc gcggtctcgg cgggcgcggc gggcctgttc
     1081 gtcctcctcg cgatggcggc ggggggcctc gccgccgtcg ccgcgggcgc ggatcgccgc
     1141 gagcgtttca cgctctccgt ggagttcgcg acgcgcaatg ccgcgatcgc cgccgccgtc
     1201 gcggtcacgc tcctcggcga cacgcggttc gccgtcttcg cgacgacgta tttcgtgacg
     1261 gaggccgtgc ccatcctcgc cgccgtggcc ctgttccggg tacgatcccg ggcagcatga
     1321 gcaaattgca gtccggtctc gggaggctgc tcgccgtcgg ccttctcctc ggcgccgcct
     1381 ccgcccgcgc gcaggatctc gagccgcgtg cctacgcccc ctcaccgaag gacgcgaact
     1441 tcgtcatcgt gaactactcg taccagtccg gcgaggtcct cttcgatccc tcgctgccct
     1501 tttcggacgt ccgcgccttc atcaacggcg tcgtcggagg gtacgggcac acgttcagcc
     1561 tcttcgggcg gatctcgtcc gtcgccgccg tggttccgta cgtctgggct tcggtggacg
     1621 gcaacgtggg cgagacgtac caggccatca cccggtccgg cctcggcgac ccgcgcctgc
     1681 gcttcgtcgt gaacctcatc ggcggcccgg ctctgccgat gaaggagttc atggcccgca
     1741 agcccgagcc gacgctcggg ttcagcctcg tcgccgtggt cccgacgggc cagtacagca
     1801 acgagaagct catcaacatc ggcacgaatc gctgggcttt tcggcccgag ttcggcctct
     1861 ccttcccggt cgggaaatgg acgctggagg gcgcggccgg cgtgtggctg ttcctcgaca
     1921 acgaccacgc ctatcccgcc gagaacgtcc gctcgcagga cccgctttac agcttccagg
     1981 tgcacgtcgg atacacggtg cgaccgggcc tctgggttgc tgccgacgcg acgtattacg
     2041 cgggcggaaa gacgtacacg aacgacgtcc caggcgacac gcgccaggcg aacacgcggg
     2101 cgggcctgac cgcctcggtc cccgttgcga ggggccattc ggtcaagctc tcggcctctc
     2161 ggggggtcga ctcccggatc ggctcccggt tcaacgcgta cggactggcc taccagtacc
     2221 tctggctcag ccgcacgggg aaataaatcg caacgtccgg gcgccccacg aggggggccg
     2281 cccttttcgt ccgctggaat gcggtcggct gacgggccgg cgggtctgcg atacgctttg
     2341 aacaaagatc gaagaacggg aagcaaaagc acatgacgag ttcgaccaga gcaagttgga
     2401 ccggcgtcgc cgcggccgcg atgtccctcg cggcggcgct cgcgtgcacg cgcggccccg
     2461 ccccggagca gaccgccgac gcgatctatg cgggcggccc catcgtgacc gtcaacgacg
     2521 cacagccgac ggccgaagtc cttgccgtca aggacggaaa gatcctcgcc gtcggcacga
     2581 aggccgggat cgagaaggtc cacaagggag cggcgacgaa ggccgtcgac ctcgcaggca
     2641 agacgctcgc tccggggttc gtggacggcc acgcgcactt cctcggcttc ggctcgcagg
     2701 cggtcggcgc caaccttctc gcgccgcccg acggaaccgt caacaccgtc gacgacgtcg
     2761 tggcgaagct gcaggagttc gcgaagggcc ccgacgtcgg gcggacgggc tggatcttcg
     2821 gcctcggcta cgacgacgcg ctgctcggac gacaccccac gcgcgacgac ctcgacaagg
     2881 tgtcgaagga cgtccccgtg atcgccgtcc acatctcggg ccacttcagc gcgatgaaca
     2941 gcgcgggtct cgcgaaggtc ggctacaccg ccgccacgaa ggacccggag ggcggggtca
     3001 ttcgccggcg gccgggatcg aaggagccga acggcgtcct cgaggagctc gcgagcatcc
     3061 ccaagatgat tccggccatc aacgcgacga agccggagga caaggactac ttcctgaaga
     3121 agggcgtcga gctcgcgaag agcttcggct acacgacagc gaacgagggc cggctcatgg
     3181 acttccagca ggccgacctc gtgagcgccg ccgggcgcgg cctcctcgac atcgacgtaa
     3241 tgtcgtacgc ggactacacg gcgaaggacg tgcttccact gatcagtcgg acatacaaag
     3301 gccgctaccg tgtggcgggg gtgaagatca ccctcgacgg ctcaccccag ggccgcacgg
     3361 cttggcggac gatcccctat ctcctgccgc ccgacggcca gaaaaagggc tacaagggct
     3421 acccggcgat cccggacacg aaggtcgtcg cggccgtcat cgacgaggcg taccagaagg
     3481 gctggccggt ccacgtgcac ggcaacggcg acgcggcgat cgaccagatg atcgaggcga
     3541 tccggcccgc ccagcagaag tacggccctg ccgatcggcg cgccaccctc attcacggcc
     3601 agttcctgcg caaggaccag atcccggccc tcaaggagct tcaggttttc ccgtcgctct
     3661 tcccgatgca cacgttctac tggggcgact ggtacgacca gatcatcggg ccggagctcg
     3721 cccagcagat ctcgcccatg cgctcggtgc tgaacgccgg gatgatcgcg accagccaca
     3781 cggacgcgcc ggtcgcgctc ccgaacctga tgcaggtgat gtgggcgacc gtcaaccgca
     3841 cgtcgcgcag cggcaaggtg atgggtcccg gcgaacggct caccccgatc gaggcgttga
     3901 aggcgatcac gctgtggggc gcccacgagc acttcgagga gaaggcgaag ggctcgctcg
     3961 aggccggaaa gctggcggac atggtgatcc tgtccaacaa cccgctcacg atcgacccgg
     4021 cgaagatcaa cacgatccag gtgatggaga cgatcaagga aggcaagacc gtctacgcgc
     4081 gcaagtaggc cggcggccgt cgacgaagga gatcaggatg agagcgaatc gcagaaacgc
     4141 cctcttgggc gcctcggccg cgacggccgt cgccgtcacg ctcgccgcga gcgcgctcgc
     4201 cgaggaggcg agccttcagg acctcgccaa ggcggtccag ggactgccgc aggtcgtgat
     4261 ctacacggcg aaggagatcg tcacgctcga ccccgcgaag cccaccgcgc aggcggtggc
     4321 ggtggtcggc ggccggatcc tcggcacggg ctcgctcgag gacgtgaagg cggccgtggg
     4381 caagcagccg tacacggtga acaccacgtt cgccaaccag gtgatcgtgc ccggcttcat
     4441 cgcccagcac gaccaccccc tcctcgccgc gctcacgatg acgagcacga tcatcgcgat
     4501 cgaggactgg gtgctgccgc agggcacgtc gaaggcggcg aagaaccgcg gcgagtatct
     4561 gaagcggctc gccgaggcga acgccgccgt caaggacccg aacgagccgc tcgtcgcctg
     4621 gggataccac cagtacttcc acggcgagct gaagaaggcg gacctcgacg cgatcagcgc
     4681 cacgcggccg atcctcgtat ggcaccgctc ggcgcacgag ttctacctga acaccgccgc
     4741 cgagaagaag tacggcgtga ccaaggagtg gttcgagccg ctctccgcca gcgtcaagaa
     4801 gcagtccgac ttcccgaacg cccactactg ggagcagggc gcgttcgcgg tcatgccgaa
     4861 gatcgcctcc gcgatcgcga cgcccgagaa gctgcgggcc ggcctcgagt tcgtgaagag
     4921 ctactaccac acgaacggcg tcaccctcgg ctgcgagccc ggcggcctcg cctcgaagag
     4981 cctgcaggac gctcagaacg ccgttctcag cgactcgtcc tcgcccttcc gcttctactt
     5041 cctggccgac ggcaagtcca tcaccgccac cgtccccgac gagaaggtgg cggaggaaac
     5101 cgggaagctc ctctcctggg gccaggggat gacggcctac ctgccgaagc aggtgaagct
     5161 cttcgccgac ggggccatct tcagccaggc catgcaggtg acgcgcggct acacggacgg
     5221 ccacaagggc gagtggatga tggacctcga tttcttcgcc cggacgttcc gtgtgtactg
     5281 ggatctcggc taccagctcc acgtccacgt caacggcgac gccgggctcg acatggtcct
     5341 cgaccagctc gaggcgaacc tgcgccgcaa cccgcggaac gaccaccgca ccgtgatcat
     5401 ccacttcgcg gtctcccggc cggagcaggt ggcgcggatc aagcgcctcg gcgcgatcgt
     5461 cagcggcaac ccgtactacc cggtgtccct cgccgacaac taccgcggca acggcctcga
     5521 ccccgagcgc gccgacgcga tggtgcggat gggcgacgtc gagaaggccg gcgtgtcgtt
     5581 ctcgttccac tccgacatgc cgatggcccc cggccagccg ctgctcctca tgtggtcggg
     5641 cgtcaatcgc atcacgaacg acggcaacgt gcgcgggccg gagcagcggg tgagccgcct
     5701 cggcgcgctc aaggcggtga cgctcgacgc ggcctactcg ctgcagatgg agagggaagt
     5761 cggcagcatc gtttcgggca agctcgcgaa cttcacgatc ctcgcggaga acccgctgac
     5821 cgtggacccc gcgaagatca aggacatcgc ggtctggggc accgtcagcg aggggcgcgt
     5881 cctgccggtg aagcgggccg ccgccgccgg gccgaaggcg gccctcggcc cggttcccga
     5941 cgccccggcg ctcgccgcgg cgatcgaggc cgatcgccac gaccacgaga acggcgcgca
     6001 cggcgacgcc tgcacggcgg cccgcggcct cgcctcggcc gttgccatgg aggcgtccaa
     6061 cccatccagg ccagccatgt gaggcacgtg ctgcgtcagg gcctcgccca gaccggcgac
     6121 gaccacgcct gttcgccgtc ggtctgggtg acacgcacgt accagcttgt ctctcccgcc
     6181 gcgtcgggcg agtcctgcac gctgaacgag aggcgccgcg cgtcctccgt cttcagccag
     6241 ttcacgccct gcgggtcctt cgggtcgtag aggacgtact cggctgccgc ctcgccggga
     6301 gcctggcgca tccgcaccgc ggcgaacgtg tcgcggaggt tccggacgat ctcgatgcgg
     6361 tcgatcgggg ctgtcccctc gacgcgcacg gccagcgtca tcgtcccctt ccccgcgagg
     6421 atcgagccca tcggggcgcc gtccgccgtg aactcgagga cgatgcgcgc gccggtcgtc
     6481 gcgtagttcc ggcgattccg gatcgcgtcg aagagggacg cgcggtccag ccgctcggcg
     6541 aggaccgccg tgagcccgcc ctcgccgggc gagaggtggt gcgtgtcgct cgcgcccatg
     6601 acgcccacgt ggcggccgag gcgcagcgcg tcctggtact ggtggcccgg cacctgccgg
     6661 cgcccttcgc gcgggttgcc gtagaactcg taccggccgt gccaggaggc gatctcgaag
     6721 ttcggctcca gctcctcgtc gtgcggggcc gccgggttcc agcgccccca gagcgcgtgc
     6781 cccgtgtggt gcggcgacat cacggcgccg tgctcgcggg cgtgccggta gagcttctcg
     6841 atcgtgtcgc tgtcccggtc ggtggagcgg tgcgtgtgct ccggcccgtc gtccttgtag
     6901 aggaggacgc ggtggccgaa gctcgagccc gccgtccact cccagcccgt gaggctcacg
     6961 aagcggcccg gcgcgtcgaa cgcgcccgcc gtcgcgcgca gctccgcgaa ctccgacggc
     7021 tggaggaggc ccatctcgga gtggtcggag acgacgtcga agtcgaggcc gagccggtcg
     7081 cgcgagagcg cgaggagctg atccgggtgg cccgtcttcg cgtccgagag gatgctgtgg
     7141 ctgtgcgggt cgccgaagta gacgtgccag cggccgccgc ccgcggtcgc ggtgcgcggc
     7201 tcggggccgg gc
//
LOCUS       DAOMFY010000201         7208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3317_length_7208_cov_6.551796, whole genome shotgun sequence.
ACCESSION   DAOMFY010000201 DAOMFY010000000
VERSION     DAOMFY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3317_length_7208_cov_6.551796"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLB01_14235"
     CDS             <1..230
                     /locus_tag="PLB01_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HQQ78509.1"
                     /translation="TPSPPPLPYDLLWRERVVRSVANATRADGREFLAGAARLPVRTH
                     VEVFPLENANEALLALKTDAIRGAAVLTVRR"
     gene            complement(227..763)
                     /locus_tag="PLB01_14240"
     CDS             complement(227..763)
                     /locus_tag="PLB01_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQQ78510.1"
                     /translation="MKTARDVMIHPVFAVREDWTVHELATFFSEKGISGAPVLDGSGR
                     LTGVVSLTDIVDQVTREREPDARVSRAWEKRFNPEDLKGLAIEEGGRLVAEIMTPTFF
                     TIPDTTPLPKIARTMVAGRIHRLLVTRQGHVVGIVTTLDLLRAFAGLGQKARGRAPVK
                     AAPTPAPGAKKARLSRLT"
     gene            851..1531
                     /locus_tag="PLB01_14245"
     CDS             851..1531
                     /locus_tag="PLB01_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78511.1"
                     /translation="MTLPEAPGLVARFRVLFAEDPRTAFREWFVLQEHLRERKEAEVS
                     ERSRALASDLLDMRGMLDFSSNEEKAVFLHNLAVFFGSRGPAEDLHSSLLLFEESSSA
                     GYGSDDLEALARLDHNRGNALQNLARSREDLVEALACYGRALEIRDATRPIARGVTLH
                     ARGLALRTLAEVAGAAGPEERAALLRSAISSFEESLSLRASEDLVRGVEETSRALDEA
                     RRALEAGC"
     gene            1542..2957
                     /locus_tag="PLB01_14250"
     CDS             1542..2957
                     /locus_tag="PLB01_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ78512.1"
                     /translation="MARRILVCDDEADIRESLRTLLTEEGYEVTAVASGVNAVGEAPD
                     HDAVLLDIKMPGQDGLDTLAKLRARGVATPVVMISGHGDVKTAMDAVRAGADDFLEKP
                     LSSEHVLNALRRVLDSSRLARENLELKSRLGIGRIVGSSEPMRRLLADIAKAAPTPAT
                     VLIVGPSGTGKELVARAIHDASLVRTAPFVQVNCAAIPDTLIESELFGHERGSFTGAD
                     RKQTGKFVEADGGTIFLDEVGDMSASAQAKVLRVLQEGEVEPVGSPRVVKVKVRVVAA
                     TNRDLTTLIAQGKFREDLYYRLNVLVLRTPALAERREDVPALVTFFAEQFAKTNNYRP
                     KRFTDEALEALAARAWPGNVRELKNHVERLMIMSDADVLDAADVDGAAAPAAEPGPVT
                     VRGGKVVDPAAAGVWERLVAIGTLQEFQDETEKLFLVAKLAENGGNVTRTAEAIDTPR
                     SNLYKKIDRYGLRRDRDGSEP"
     gene            2954..3571
                     /gene="pyrE"
                     /locus_tag="PLB01_14255"
     CDS             2954..3571
                     /gene="pyrE"
                     /locus_tag="PLB01_14255"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQQ78513.1"
                     /translation="MTPEAVRRHFEETGALLSGHFKLSSGLHADRYLQCARVLQHPDR
                     AGVLGAALAALLSPEKPEVVVSPAMGGVIIGQEVGRGLGVRAIFTERVDGVFSLRRGF
                     ALEPGERVAVIEDVVTTGKSTKEVLEVLRKAGAVPVVCGSIVDRRAAAEKAPAVDGIP
                     YRALLPLEVPAWEASACPMCARGEPVVAPGSRHLAVPPGGPPAPR"
     gene            complement(3576..4481)
                     /locus_tag="PLB01_14260"
     CDS             complement(3576..4481)
                     /locus_tag="PLB01_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQQ78514.1"
                     /translation="MSDRSASSWALLSIAAAVVTIGLKAYAWKVTGSVGLLSDAAESG
                     VNLLAALVAFWALRRASRPADSTYAFGQTKAEYFASGFEGLMILAASVAIAVTAVERL
                     RNLQPIENVGLGLGISVLASGVNGVVAWILMREGKRLRSITLRADAQHLLTDVWTSAG
                     VLVGVFLVKLTGWLILDPIVALLVAANIVYTAVRLLQETGYGLLDKAIGEDEQAALSA
                     ALAPFHERGIVLHDIRTRRAGPRHFIQMHVLVPGSWTVKRGHDVCEEIERGVAAALPG
                     STVTTHLEPIEDPAAWDDRGLDRHL"
     gene            4576..5658
                     /locus_tag="PLB01_14265"
     CDS             4576..5658
                     /locus_tag="PLB01_14265"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQQ78515.1"
                     /translation="MRALVCDGPGSAVLRDVAVPAPGPGEVLVRVAVALTCGTDLKIV
                     RRGHPKIPFPLTLGHEWAGVVEKAGRGAAFSPGTRVTSAVSSPCGACEACRAGRENLC
                     ASAFDRPLFGAFADFLLVPERLVKNGLRRIPDGMPDETAALLDPLASAVRGLNRVGDA
                     RGKDVLIVGTGPIAALLFFLLRGKEGEETGRAAERRVVIAGRRAERLAFFERNLAEVA
                     HIGPSSRAGEAGAPPPVFTFKEVFSSLSPSSSFSLVIDTTGDPGVIEALPALASDGGT
                     VLLFAGLPAEARVAIDAHAVHYREVSIVGSFHYTPREVDEALGLLSGGAIPAAEIVSA
                     RRPLAEWEAAFDVARAGAALKVGVFP"
     gene            5664..6677
                     /locus_tag="PLB01_14270"
     CDS             5664..6677
                     /locus_tag="PLB01_14270"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQQ78516.1"
                     /translation="MRAAVAHSVDRVEVVTREVPPIGPGEILVAMKAVGICGSDTTGW
                     YVETKAPAILGHEPAGVVVEAGEGTRFEPDDRVVVHHHAACGRCRFCLAGEDVQCAEW
                     RKNRLDPGGLAEYVRIDAAAVRADVLFLPDEVSFEDGALVEPVACAVKAVRRAAVHRG
                     DTAAVIGLGANGVALGLVARAAGAWKLVGCDPDPARRAHALRLGFDQVAEPGEDFSPK
                     VKRVSADGADAVFVLPTAEEAVRAALAAAAPAGRIVFYSPVQPGKRWTFEPHLPYLRD
                     LSMLFSYSSSARDLAEALQLIRRGVVRASDLVTHRVPLADAPEAFRLARRGGDVLKVM
                     VTI"
     gene            6674..>7208
                     /locus_tag="PLB01_14275"
     CDS             6674..>7208
                     /locus_tag="PLB01_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HQQ78517.1"
                     /translation="MKKKRLDVLLVERGLAESRARAEALILAGRVAVAGKERVKPGTP
                     VDDAAEVSVGEPEHPWVSRGGVKLAFGLTHFGIDPAGRVCLDVGASTGGFTDVLLARG
                     AARVYAVDVGWGQLHAKLRGDPRVVLREKVNARLLSRAEVPEPPSLLVGDLSFISLTL
                     VLPAAVPLLAPSADVLVL"
BASE COUNT          986 a         2530 c         2613 g         1079 t
ORIGIN      
        1 cgaccccgtc gccgccgccg ctgccctacg acctcctctg gcgcgagcgc gtcgtccgga
       61 gcgtcgcgaa cgcgacgcgc gccgacgggc gcgagttcct ggcgggggcc gcgcgcctgc
      121 cggtgcgaac gcacgtcgag gtcttccctc tcgagaacgc gaacgaggcg ctcctcgcgc
      181 tcaagaccga cgcgatccgg ggagcggcgg tcctgacggt acgccgttag gtgaggcgcg
      241 acaggcgcgc tttcttcgcc ccgggcgcgg gcgtcggcgc cgccttgaca ggggcgcggc
      301 cccgggcttt ctggcccagg ccggcgaacg cgcgcaggag gtcgagcgtc gtgacgatgc
      361 cgacgacgtg gccctgccgc gtcacgagga gccggtggat gcgcccggcc accatcgtcc
      421 gcgcgatctt cggcaggggc gtcgtgtccg ggatcgtgaa gaacgtcggc gtcatgatct
      481 cggcgacgag gcggccgccc tcctcgatcg ccaggccctt gaggtcctcg gggttgaacc
      541 gcttctccca cgcccgggag acgcgcgcgt cgggctcgcg ctcgcgcgtc acctggtcga
      601 cgatgtcggt caggctcacg acgccggtga ggcggccgga accgtcgagc accggggctc
      661 ccgaaatgcc cttctccgag aagaaggtcg ccagctcgtg gacggtccag tcctcgcgca
      721 ccgcgaagac gggatggatc atgacgtcgc gggcggtctt catctcgtcg ttcccgcgac
      781 gagcttcccg cgccgccggc gcgcacgaca tagcgcagct gaatcatcgc ggtgcgcgta
      841 ggatggcggc gtgacgctcc ccgaggcgcc cgggctcgtg gcgcgcttcc gcgtgctctt
      901 cgcggaggac ccgagaacgg cgttccgcga gtggttcgtg ctccaggaac acctcaggga
      961 gagaaaggaa gcggaggttt cggagagatc acgtgccctc gcgagcgacc ttctcgacat
     1021 gcgcgggatg ctggatttct cttcgaatga ggaaaaggcg gtcttcctgc acaacctcgc
     1081 cgtcttcttc ggctcgcggg gacccgccga agatcttcat tcctctctct tgctcttcga
     1141 agaatcttcc agcgccgggt acgggagcga cgatctggag gcgttggccc gcctcgacca
     1201 caacagggga aacgcgctcc agaacctcgc gcggtcgcgg gaggacctcg tcgaggcgct
     1261 cgcgtgctac gggcgggcgc tcgagatccg cgacgcgacc cggcccatcg cacggggcgt
     1321 gacgcttcac gcgaggggcc tcgcgctccg gacgctcgcc gaggtggcgg gcgcggccgg
     1381 cccggaggag agagccgcgc tcctccggtc ggccatctcc tcattcgaag aatctctttc
     1441 tcttcgtgcc tccgaggatc tcgtccgcgg cgtcgaggag acgtctcgcg ccctggacga
     1501 ggcgcggcgc gccctcgaag cgggctgcta ggatgatcgc gatggcgcgc cgcatcctcg
     1561 tctgcgacga cgaagccgac atccgggagt ccctccgcac gctcctgacg gaggagggat
     1621 acgaggtcac ggcggtcgcc tccggcgtga acgcggtggg cgaggctccg gaccacgacg
     1681 cggtcctcct cgacatcaag atgcccgggc aggacggcct cgacacgctc gcgaagctgc
     1741 gggcgcgggg cgtcgcgacg ccggtcgtca tgatctcggg tcacggcgac gtcaagacgg
     1801 cgatggacgc cgtccgcgcg ggcgcggacg acttcctcga gaagccgctc tcgtccgagc
     1861 acgtcctgaa cgccctccgc cgcgtcctcg actcgagccg cctcgcgcgc gagaacctcg
     1921 aattgaaatc gcgcctcggg atcggccgca tcgtggggtc gagcgagccg atgcggaggc
     1981 tcctcgcgga catcgcgaag gcggcgccga cgcccgcgac ggtcctcatc gtcggaccga
     2041 gcggcacggg caaggagctc gtcgcgcgcg cgatccacga cgcctctctc gttcgcacgg
     2101 cgccgttcgt tcaggtcaac tgtgccgcga tccccgacac gctcatcgaa tccgagctct
     2161 tcgggcacga gcgcggctcg ttcacgggag ccgaccgcaa gcagacgggc aagttcgtcg
     2221 aggcggacgg cggcacgatc ttcctcgacg aggtcgggga catgtcggct tccgcgcagg
     2281 caaaggtgct ccgcgtcctg caggagggcg aggtcgagcc cgtcgggagc ccgcgcgtcg
     2341 tcaaggtgaa ggtccgcgtg gtcgccgcga cgaaccgcga cctcacgacg ctcatcgcgc
     2401 aggggaagtt ccgggaggac ctctactacc gcctgaacgt cctcgtcctg aggacgccgg
     2461 ccctcgcgga gcgccgcgag gacgttcccg cgctcgtgac gttcttcgcg gagcagttcg
     2521 cgaagacgaa caactacagg ccgaagcggt tcacggacga ggcgctcgag gcgctcgccg
     2581 cgcgcgcgtg gccggggaac gtccgggagc tgaagaacca cgtggagcgg ctcatgatca
     2641 tgtccgacgc ggacgtcctc gacgccgccg acgtggacgg cgccgccgcg cccgcagccg
     2701 aacccggccc cgtcaccgtc cgcggcggca aggtcgtgga cccggcggcc gcgggggtct
     2761 gggagcggct cgtggcgatc ggcacgctcc aggagttcca ggacgagact gagaagctct
     2821 tcctcgtcgc gaagctcgcg gagaacggag gcaacgtgac gcgcaccgcc gaggcgatcg
     2881 acacgcctcg ctcgaacctc tacaagaaga tcgaccgtta cggcctgcgc cgcgaccggg
     2941 acgggagcga gccgtgacgc ccgaagccgt gcgccgccac ttcgaggaga cgggcgcgct
     3001 cctgtcgggc cacttcaagc tctcgtcggg ccttcatgcg gaccgctacc tccagtgcgc
     3061 gagggtcctc cagcacccgg accgcgccgg cgtgctcggt gccgcgctcg cggcgctcct
     3121 ttcgccggag aagccggaag tcgtcgtgtc gcccgcgatg ggcggcgtca tcatcggcca
     3181 ggaggtcggt cgcgggctcg gcgtgcgcgc gatcttcacg gaacgcgtgg acggcgtctt
     3241 ctccctccgc cgcggattcg ccctcgagcc tggagagcgc gtcgccgtga tcgaggacgt
     3301 cgtcacgacg gggaagtcga cgaaggaggt cctcgaggtc ctccgtaagg cgggcgccgt
     3361 gcccgtcgtg tgcggctcga tcgtggaccg gcgcgccgcc gcggagaagg ctccggccgt
     3421 cgacgggatc ccgtaccgcg cgctcctgcc gctcgaggtc ccggcctggg aggcctccgc
     3481 gtgtcccatg tgcgcgcgcg gggagcccgt ggtcgcaccc ggctcgcggc atctcgcggt
     3541 tcccccgggc gggccgcccg cgccgcgctg acccgtcaga ggtggcggtc gaggccgcgg
     3601 tcgtcccacg cagccgggtc ctcgatgggc tcgagatggg tcgtgaccgt gctgcccggc
     3661 agcgcggcgg cgacgccccg ctcgatctct tcgcagacgt cgtgcccccg cttgacggtc
     3721 cacgagcccg ggacgaggac gtgcatctgg atgaagtggc gcggccccgc gcggcgggtc
     3781 cggatgtcgt gcaggacgat cccgcgctcg tggaacggcg cgagggcggc cgagagcgcc
     3841 gcctgctcgt cctcgccgat cgccttgtcg aggaggccgt acccggtttc ctgcaggagc
     3901 cgcaccgccg tgtagacgat gttcgccgcg acgagcagcg cgacgatggg gtcgaggatc
     3961 agccagcccg tgagcttcac gaggaagacg ccgacgagga cgccggcgga cgtccagacg
     4021 tccgtgagga ggtgctgcgc atcggcccga agcgttatcg aacggagcct cttgccctcg
     4081 cgcatgagga tccatgcgac gacgccgttc acgccggaag ccaggaccga gatcccgagg
     4141 cccagcccga cgttctcgat cggctggagg ttgcggaggc gttcgacggc cgtcaccgcg
     4201 atcgcgacgg aggccgcgag gatcatgagg ccctcgaagc cgcttgcgaa gtactcggcc
     4261 ttcgtctggc cgaacgcgta ggtggagtcg gcggggcggc tcgcgcggcg gagagcccag
     4321 aacgcgacga gggccgcgag gagattcacg cccgactcgg ccgcgtccga gaggagaccg
     4381 accgagcccg tgaccttcca ggcgtaggcc ttcaggccga tcgtcacgac ggcggcggcg
     4441 atcgacagga gagcccagct cgacgcggag cggtcgctca tggacgcgag gagactgcgt
     4501 cgggcccgcc cgcgcgtcaa gcgaagcacg gtccctgcga cgcggtctta agcgcactcc
     4561 tgttacgctt cttccgtgcg cgcactcgtt tgcgacggcc ccggaagcgc ggtcctgaga
     4621 gacgtggcgg tgcccgcgcc cggcccgggc gaggtgctcg tccgtgtcgc cgtcgcgctt
     4681 acatgcggga cggacctcaa gatcgtgcgg cgcggtcatc cgaagatccc gttcccgctg
     4741 accctcggcc acgagtgggc gggcgtggtc gagaaggccg gccgtggcgc ggccttctcg
     4801 ccgggcacgc gcgtcacgtc ggcggtctcg tcgccttgcg gcgcgtgcga ggcctgccgc
     4861 gcgggtcgcg agaacctctg tgcgagcgcc ttcgaccgcc ctctcttcgg cgcgttcgcg
     4921 gacttcctcc tcgtgcccga gcgcctcgtg aagaacggcc tgcggaggat cccggacggc
     4981 atgcccgacg aaacggccgc gctgctcgac ccgctcgcga gcgccgtgcg cgggttgaac
     5041 cgcgtgggcg acgcgcgggg caaggacgtc ctcatcgtgg gcaccggccc gatcgcggca
     5101 ctgctgtttt ttcttcttag ggggaaagag ggagaagaga cggggagggc cgcggaacgc
     5161 cgcgtcgtca tcgcaggaag gcgggcagag agactcgctt tctttgaaag gaatttggcg
     5221 gaggtcgctc acatcgggcc ctcgtcgcgc gcgggcgaag ccggcgctcc gcctcccgta
     5281 ttcaccttca aagaagtctt ctcttctctt tctccctctt catccttttc cctcgtcatc
     5341 gacacgaccg gcgatcccgg cgtgatcgag gcgctgccgg cgttggcttc cgacggcgga
     5401 acggtgcttc tcttcgctgg gctgcccgcg gaagcgcgcg tcgcgatcga cgcgcacgcg
     5461 gtccattacc gggaggtctc gatcgtcggc tcgttccact acacgccgcg cgaggtggac
     5521 gaggcgctcg gcctgctctc cggaggcgcc attcccgccg cggagatcgt ctcggcccgg
     5581 cggccgctcg ccgagtggga ggccgcgttc gacgtcgccc gcgccggggc agcgctgaag
     5641 gtcggggtgt tcccgtgagg acgatgcgcg ccgccgtcgc gcactccgtg gaccgagtcg
     5701 aggtcgtgac gcgcgaggtg cccccgatcg gcccggggga gatcctcgtc gcgatgaagg
     5761 cggtcgggat ctgcggcagc gacacgaccg gctggtacgt cgagacgaag gcgccggcca
     5821 tcctcggcca cgagccggcg ggcgtcgtcg tggaggccgg ggaggggacg cgcttcgagc
     5881 cggacgaccg cgtcgtcgtc catcaccacg ccgcgtgcgg ccgctgccgc ttctgcctcg
     5941 cgggcgagga cgtccagtgc gccgagtggc ggaagaaccg cctcgacccc gggggcctcg
     6001 cggagtacgt ccgaatcgac gccgcggccg tgcgcgcgga cgtcctgttc ctgccggacg
     6061 aggtctcctt cgaggacgga gcactcgtcg agcccgttgc gtgcgccgtg aaggccgtcc
     6121 gccgcgccgc ggtccaccgc ggcgacacgg cggcggtgat cggcctcggc gcgaacggcg
     6181 tggcgctggg cctcgttgcg cgcgccgcgg gggcgtggaa gctcgtcggc tgcgatccgg
     6241 accccgcgcg aagggcccat gcgctgcgac tcgggttcga tcaggtggca gagccgggag
     6301 aggacttttc tccgaaggtg aagagggtct cggccgacgg agcggacgcc gtcttcgtcc
     6361 tgccgaccgc cgaagaggcg gttcgcgccg cgctcgccgc cgcggcgccc gcgggccgga
     6421 tcgtcttcta ttcgccggtc cagcccggaa agagatggac gttcgagccg cacctgcctt
     6481 acctgcgcga cctttcaatg ctcttttctt attcttcctc ggctcgcgac ctcgccgaag
     6541 cgctgcagct catccggcgg ggcgtcgtcc gcgcctcgga cctcgtgacc caccgcgttc
     6601 cgctcgcgga cgccccagag gcgttccggc tcgcgcgccg gggcggcgac gttctcaagg
     6661 tgatggtgac gatctgaaga agaagcggct cgacgtccta ctcgtcgagc gcggcctcgc
     6721 cgagtcgcgc gcccgcgccg aggccctgat cctcgccggg cgcgtcgcgg tcgccgggaa
     6781 ggagcgggtg aagcccggga cgcccgtcga cgacgccgcc gaggtctcgg tgggggagcc
     6841 cgagcacccg tgggtctcgc gcggaggcgt caagctcgcg ttcggcctga cgcacttcgg
     6901 gatcgatccc gcggggcgcg tctgcctgga cgtcggcgcg tccaccggcg ggttcacgga
     6961 cgtgctcctc gcgcgaggag ccgcgcgggt gtacgccgtg gacgtcggct gggggcagct
     7021 ccacgcgaag ctgcgcggcg accctcgcgt ggtcctccgc gagaaggtca acgcgcgtct
     7081 cctgtcgcgc gcggaggtcc ctgagccgcc gtccctcctc gtcggggacc tctcgttcat
     7141 ctcgctgacg ctcgtcctgc ctgcggccgt tccgctcctt gctccgtcgg cggacgttct
     7201 cgtcctcg
//
LOCUS       DAOMFY010000202         7203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3320_length_7203_cov_8.268327, whole genome shotgun sequence.
ACCESSION   DAOMFY010000202 DAOMFY010000000
VERSION     DAOMFY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3320_length_7203_cov_8.268327"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PLB01_14280"
     CDS             complement(<1..1501)
                     /locus_tag="PLB01_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78518.1"
                     /translation="MSPLVLSRVRAAAVLLMAGPLLAQAPPAATPAPTPRPGVTAAEE
                     VTVLLLDVVAVDGKNRPVFGLAAADFEVRVAGKVQTIDSFEPPREPRGPATAREGRPD
                     SEERIAGTTTSFDPKGPVRHVLVWVDLEQLPRVSILDTAKALHKTFDHAPSGRYGFAT
                     HFGGTSARVWDADSVDAFLTEADRMSAEVAEDAGGAAVKSAGAPGMASPRGMESPLQY
                     EARRLYEDQLIRDLIATENANGDTRLIFGAVSQYLTAERRRVKTLVEDLREAAQRFSA
                     LDGPRHLFYVSEGFERVPGFNFLARLRAEETASVRPVASGGSVQGLPSGPSRGRTGVG
                     SFPTIPGAPGASQELGAGRFVFDPSALPEADDLARFLATSGVIVHFIDPGSLGRGLPS
                     AADRYAFNASLRQDEARNLQDTPMRYVQETGGIARVSTNDMTAALEGLLDATAATYRL
                     GVRLSGVDPKKTYSVRVATRKAGVNMLARSAFKPAGPSPKAAAALAEDAR"
     gene            complement(1536..1859)
                     /locus_tag="PLB01_14285"
     CDS             complement(1536..1859)
                     /locus_tag="PLB01_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78519.1"
                     /translation="MNPSALFEVKGVMGIAFLGFAGLLLFILVVSFRSRATVFCQYLK
                     AMTGIELKPRDVGSVFKEKGQDGVRELFLDLIIKEDLRQTGPLQIPPELRAGKPGSPK
                     SLSQG"
     gene            complement(1949..3070)
                     /locus_tag="PLB01_14290"
     CDS             complement(1949..3070)
                     /locus_tag="PLB01_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQQ78520.1"
                     /translation="MRLPLSPSPSKKLFLFFFFLFAAENARADAKDVKLDTIRLPPGF
                     RISLYAEVPGARSMTMGTGGTLFVGTREGSVYAAVDAGDGTRAGEVLTLAKNLDEPNG
                     VAFRDGALYVAEISRILRFDGIEARLKNPGRPAVVTDALPKDAHHGWKFIAFGPDGWL
                     YVPVGAPCNICRRSDPYASITRMKPDGTHLEVFARGVRNTVGFDWDPATKELWFTDNR
                     RDWMDDDTPPDELNHAPAAGMHFGYPWCHGGDVKDPEFGKERPCSEFVKPALRFQAHV
                     APLGMRFYAGSAFPAEYRGRIFVAQHGSWNRKKKVGYRVVTIPIKDGKSGAEAVFAEG
                     WLEGEKAWGRPVDVLVRPDGSLLVSDDKAGVVYRISFSR"
     gene            3131..3796
                     /locus_tag="PLB01_14295"
     CDS             3131..3796
                     /locus_tag="PLB01_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HQQ78521.1"
                     /translation="MPLTDDTPPPRLPRPLDPVEIRVLGALLEKQQATPEYYPLTLHA
                     LVAACNQKTNRDPVTDLDEAAVLAALERLREHVLVWKTGGARAERWEQNVDRRWGLDA
                     AGMAVMTLLLLRGEQTPGELRGRSDRLHAFATPGDVEDALRSLAAGPEPLVAETARRP
                     GQKESRWTHLVGGAAAAALASEPARTPAPHDLSSRLASLEQAVAALARDLGELKRKLG
                     EEP"
     gene            3793..5580
                     /locus_tag="PLB01_14300"
     CDS             3793..5580
                     /locus_tag="PLB01_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQQ78522.1"
                     /translation="MNPLVDDRGLDFLLWEVHDAASLLAYPHFADHSRETFDAYVGLC
                     RRFGRETLFPAYRTLDAEPPRFESGRVTVHPLLHTIWPRLVEIGVVAASRPFDVGGAQ
                     LPLTVATAAHLYVMAGCCAVYSYAGLTTGAAHLVETFGSEALKETYLAPLYEGRWTGT
                     MALTEPQAGSSLADVATRATPAGGHFLLSGSKIFISGGDHDLAENVVHLALARIDGAP
                     AGIRGVSLFVVPKRRAENGALVPNDVTTAGLVHKIGWKALPAAVLSFGEAGDCRGWLV
                     GEANQGVPYMFQMMNAARISVGADAVATASVAYQESLAYARTRPQGRPAGSRDPGTPQ
                     VPIVEHADVRRMLLRQKAIVEGGLSLILGVAKLEDASRYAADDAERERAQLLLDLLTP
                     VAKSFPAEKGFESTCLAVQVHGGYGYTSEYLPEALWRDQKLNSIHEGTTGIQALDLLG
                     RKAVAQNGAALAALGREVAAACARAERAGVAPDWIAAVRGTGERIGALTAALGKAGAG
                     GDVEGMLRHAADYLDLFSTFVVAWQWLLQAAVAREAIDAGRGPEAFYEGKLAAAQYWI
                     RTELPKAAALATVCGENEDSYARMRSDWF"
     gene            complement(5585..5938)
                     /gene="speD"
                     /locus_tag="PLB01_14305"
     CDS             complement(5585..5938)
                     /gene="speD"
                     /locus_tag="PLB01_14305"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HQQ78523.1"
                     /translation="MKPLGTHFLLDVAGAPFATLDDPVLVETLLVDAVKQMGAKVLGI
                     HIHRLSPQGVSGVVVISESHLTIHTWPERGEAAVDLFTCGDAGRARVAVDALAKALGA
                     TKARVEERVRGAGDA"
     gene            complement(5935..7017)
                     /locus_tag="PLB01_14310"
     CDS             complement(5935..7017)
                     /locus_tag="PLB01_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78524.1"
                     /translation="MTSNWLFSRRADLLLFGGSAVLALALLALGMAAGILHGDAPPWL
                     FLLAIVGVDVAHVWSTGWRVLADGADAHERLALYVGVPLLAWAAGVAAYSVSALFFWR
                     VLAYLAVFHFVRQQYGWVALYRRKNGEGEEGSWGRRLDAAAVYGATLTPLLFWHAHLP
                     RRFQWFLQGDFLPGLPPVVSRAAFVLYAAVLAAWAVKEIARARAGRRVSWGKVLVVLT
                     TVATWFLGIVVLDSDYAFTVTNVFVHGIPYMGLVWFTSKARAEGRRAAGAPPAAADRW
                     VASLPLFLLPLLAIAFAEEWGWDRLVWHDNPRFFPGAAAEPGALALVLLVPLLALPQA
                     THYVWDAFLWKVKPANAGAVQALGIP"
BASE COUNT         1051 a         2579 c         2614 g          959 t
ORIGIN      
        1 ggcgggcgtc ctccgccagc gccgccgccg ccttcgggga cggccccgcg ggcttgaacg
       61 cggagcgcgc gagcatgttc acgcccgcct tgcgcgtcgc gaccctgacg gaataggtct
      121 tcttcgggtc cacgcccgag agcctcacgc cgagcctgta cgtcgcggcg gtcgcgtcga
      181 ggaggccctc gagcgcggcg gtcatgtcgt tcgtcgagac gcgggcgatg ccccccgtct
      241 cctgcacgta ccgcatcggc gtgtcctgca ggttgcgcgc ctcgtcctgg cggaggctcg
      301 cgttgaacgc gtaccggtcc gcggcggaag ggagcccgcg gccgagcgag ccggggtcga
      361 tgaaatggac gatcacccct gacgtcgcga gaaagcgggc gaggtcgtcg gcctcgggga
      421 gcgcgctcgg gtcgaacacg aaccgtccgg cgccgagttc ctgcgaagcc ccgggcgcgc
      481 ccggaatcgt cgggaagctc ccgacgcccg tgcggccgcg cgagggtccg gacggcaggc
      541 cctgaacgct gccacccgac gcgacgggcc gcacgctcgc ggtctcctcg gctctcaggc
      601 gcgcgaggaa gttgaagccc ggcacgcgct cgaagccctc ggagacgtag aagaggtggc
      661 gcggcccgtc gagcgcggag aatcgctgcg cggcctcccg gaggtcctcc acgagcgtct
      721 tcacgcggcg gcgttcagcc gtgaggtact gggagacggc cccgaagatc aggcgggtgt
      781 cgccgttcgc gttctcggtc gcgatgaggt cccggatgag ctggtcctcg tacagtctcc
      841 gcgcctcgta ctggagcggg gactccattc cgcgaggtga cgccattccg ggcgcgcccg
      901 cggacttgac cgccgcgccg ccggcgtcct cggcgacctc ggcgctcatc cggtcggctt
      961 cggtgaggaa tgcgtcgacc gaatccgcgt cccacacgcg cgcggacgtc ccgccgaagt
     1021 gcgtcgcgaa gccgtaccgg cccgagggcg cgtggtcgaa cgtcttgtgc agggccttgg
     1081 ccgtgtcgag gatcgacacg cgggggagct gctcgaggtc cacccagacg aggacgtggc
     1141 gcacggggcc cttcgggtcg aacgacgtcg tcgtgcccgc gatgcgttcc tcggagtcgg
     1201 gccggccttc cctcgccgtg gccgggcccc ggggctcgcg cggcggctcg aacgagtcga
     1261 tcgtctggac cttgccggcg acccggacct cgaagtccgc cgcggcgagg ccgaagaccg
     1321 ggcggttctt cccgtcgacg gccacgacgt cgaggagcag cacggtgact tcttccgccg
     1381 ccgtcactcc gggccggggc gtcggtgcag gcgtggccgc gggcggcgcc tgcgcgagga
     1441 gcggccccgc catgagcagg acggcggcgg cgcgcactct cgagaggacg agcgggctca
     1501 tcgcggaacc ggccagcacg cctcgtgccg cccggtcagc cctgcgacag ggatttcgga
     1561 ctgccgggct ttcccgcgcg cagctcgggc ggaatctgga gcggacccgt ctgccgcagg
     1621 tcttccttga tgatgaggtc gaggaagagc tcgcgcacgc cgtcctgccc cttttccttg
     1681 aagacgctgc cgacgtcgcg cggcttgagc tcgattccgg tcatcgcctt gaggtactgg
     1741 cagaacacgg tcgcgcgcga ccggaacgac acgacgagga tgaagaggag gaggccggcg
     1801 aacccgagga aggcgatccc catgacgcct ttgacctcga aaagggccga gggattcatg
     1861 cgggagctct ccgcgccgga cgagccggtt tcatgaacgt ctccttctcc ccgcgagcct
     1921 gctcatcata tcgcgagccg ggcccggttc agcgggagaa ggagatcctg tagacgactc
     1981 ccgccttgtc gtccgagacg aggagtgacc cgtcgggccg gacgaggacg tccacgggac
     2041 ggccccacgc cttctcgccc tcaagccagc cttccgcgaa gacggcctcg gcgccggatt
     2101 ttccgtcctt gatcgggatc gtcacgacgc ggtagccgac cttcttcttc cggttccacg
     2161 agccgtgctg ggccacgaag attcggccgc ggtactcggc cgggaaggcg gagcccgcgt
     2221 agaagcgcat cccgagcggc gccacgtggg cctggaaccg gagggccggc ttcacgaact
     2281 ccgagcacgg gcgttccttg ccgaactccg ggtccttcac gtcgccgccg tggcaccacg
     2341 ggtacccgaa gtgcatcccg gccgcgggcg cgtggttcag ctcgtccggc ggcgtgtcgt
     2401 cgtccatcca gtcgcgcctg ttgtccgtga accacagctc cttcgtggcc gggtcccagt
     2461 cgaacccgac cgtgttccgg acgccacgcg caaagacctc gaggtgcgtg ccgtccggct
     2521 tcatgcgcgt gatcgaggcg tacgggtcgc tgcgcctgca gatgttgcag ggcgcgccca
     2581 cgggcacgta gagccagccg tccggcccga acgcgatgaa cttccagccg tggtgcgcgt
     2641 ccttcgggag cgcgtccgtg acgacggccg gccggcccgg attcttcagg cgcgcctcga
     2701 tcccgtcgaa gcgcaggatg cgcgagatct ccgcgacata gagcgcgccg tccctgaacg
     2761 ccacgccgtt cggttcgtcg aggttcttcg cgagggtgag gacctcgcct gccctcgtgc
     2821 cgtcgcccgc gtcgacggcg gcgtagacgg agccttcgcg cgtccccacg aagagcgtcc
     2881 ccccggtgcc catcgtcatc gagcgcgcgc cgggcacctc cgcgtagagc gagatcctga
     2941 atccgggggg aagcctgatc gtgtcgagct tgacgtcctt cgcgtcggcg cgcgcgttct
     3001 ctgcagcaaa gagaaagaag aagaagagga agagtttctt ggaaggtgaa ggggagagag
     3061 gcagccgcat cggtcctccg ctcgggtcga attatcggct ctccgtcgcc gccgcataat
     3121 ctccgtcctc atgcccctca ccgacgacac gccgcctccc cggctgccgc gtcccctcga
     3181 tcccgtcgag atccgcgtcc tcggtgcgct gctcgagaag cagcaggcga cgccggagta
     3241 ctacccgctc acgctccacg cgctcgtcgc ggcgtgcaac cagaagacga accgcgatcc
     3301 cgtcacggac ctggacgagg cagcggtcct cgcggctctc gagcgcctgc gcgagcacgt
     3361 cctcgtctgg aagaccggcg gtgcgcgggc cgagaggtgg gagcagaacg tggaccgccg
     3421 ctggggcctc gacgcggccg gcatggccgt gatgacgctc ctcctcctgc gcggcgagca
     3481 gacgccgggc gaactgcgcg ggcggagcga ccggctgcac gcgttcgcga cgcccggcga
     3541 cgtcgaggac gcgctccggt ctctcgcggc ggggcccgag ccgctcgtcg ccgagacggc
     3601 gcgccgtccc ggccagaagg agtcgcgctg gacgcacctc gtggggggag cggcggcggc
     3661 cgcactcgcg tccgaaccgg cgcgcacgcc cgcaccgcac gacctctcct cccgccttgc
     3721 gtctctcgag caggccgtcg ccgcgctggc tcgcgacctc ggcgagctga aaaggaagct
     3781 gggagaggag ccgtgaaccc gctcgtcgac gaccgcggcc tcgacttcct cctctgggag
     3841 gttcacgacg ccgcgtcgct cctcgcgtac ccgcacttcg ccgatcactc ccgggagacg
     3901 ttcgacgcgt acgtcggcct ctgccgtcgg ttcgggcgcg agacgctgtt tcctgcgtac
     3961 cgcacgctcg acgccgagcc gccgcgcttc gagagcgggc gcgtcacggt gcacccgctc
     4021 ctccacacga tctggccgag actcgtcgag atcggcgtcg tggcggcctc gcgcccgttc
     4081 gacgtcggcg gggcgcagct gccgctcacc gtcgcgaccg cggcccacct ctacgtcatg
     4141 gcgggctgct gcgccgtcta cagctatgcg ggcctcacga ccggcgccgc gcacctcgtc
     4201 gagacgttcg ggagcgaagc cctgaaggag acatacctcg cgccgctcta cgaaggacgc
     4261 tggacgggga cgatggcgct cacggagccg caggccggct cgagccttgc ggacgtcgcg
     4321 acccgtgcga cgcccgcggg cggccacttc ctcctctcgg gctcgaagat cttcatctcg
     4381 ggcggcgacc acgacctcgc ggagaacgtc gtccacctcg cgctcgcgcg gatcgacggc
     4441 gcgcccgcgg gcatccgcgg cgtgagcctc ttcgtcgtcc cgaagcgccg cgccgagaac
     4501 ggcgccctcg tcccgaacga cgtcacgacg gccggcctcg tccacaagat cggctggaag
     4561 gcgctcccgg cggccgtcct gtcgttcggc gaggcgggag actgccgcgg ctggctcgtc
     4621 ggcgaggcga accagggcgt cccctacatg ttccagatga tgaacgcggc gcgcatctcc
     4681 gtcggcgcgg acgccgtcgc gaccgcctcc gtcgcctacc aggagagcct cgcgtacgcg
     4741 cggacgcgcc cgcagggccg ccccgcggga agccgcgacc cgggcacgcc gcaggtcccg
     4801 atcgtcgagc acgcggacgt ccggcggatg ctcctgcgcc agaaggcgat cgtcgagggc
     4861 ggactctcgc tcatcctcgg cgtggcgaag ctcgaggacg cctcgaggta cgcggccgac
     4921 gatgccgagc gcgagcgtgc gcagctcctc ctcgacctcc tgacgccggt cgcgaagagc
     4981 ttcccggccg agaaggggtt cgagtcgacc tgcctcgccg tccaggtcca cggcggctac
     5041 ggctacacga gcgagtacct gcccgaggcg ctgtggcgcg accagaagct gaactccatc
     5101 cacgagggga cgacggggat ccaggcgctg gacctcctcg gccgcaaggc ggtcgcgcag
     5161 aacggcgccg ctcttgcggc cctcggccga gaggtcgccg cggcgtgcgc gagggcggag
     5221 agggcgggcg tcgcgcccga ctggatcgcg gccgtgcgcg ggacgggcga gaggatcggc
     5281 gcgctcacgg cggcgctcgg gaaggccggc gcgggcggcg acgtcgaggg gatgcttcgc
     5341 cacgccgcgg actacctcga cctcttctcc acgttcgtcg tcgcgtggca gtggctcctg
     5401 caagcggccg tcgcgcgcga ggccatcgac gcgggccgcg ggccggaggc gttctacgag
     5461 ggcaagctcg cggccgcgca gtactggatc cgcaccgagc tcccgaaggc cgccgcgctc
     5521 gcgacggtct gcggggagaa cgaggactcg tacgcgcgga tgcggtccga ctggttctag
     5581 cggatcaggc gtccccggcg ccgcgcacgc gctcctcgac cctcgccttc gtggcgccga
     5641 gcgccttcgc gagcgcgtcg accgcgacgc gcgcccggcc cgcgtctccg cacgtgaaga
     5701 ggtccaccgc ggcctcgccg cgctcgggcc aggtgtggat cgtgaggtgc gactcggaga
     5761 tcacgacgac gccgctcacg ccctgcgggc tcaggcggtg gatgtggatg ccgagcacct
     5821 tcgcgcccat ttgcttcacg gcgtccacga ggagcgtctc gacgaggacg ggatcgtcca
     5881 gcgtcgcgaa cggcgcgccc gcgacgtcga ggaggaagtg ggttccgagc ggcttcacgg
     5941 gatccccagc gcctggacgg cgcccgcgtt cgcgggtttg accttccaga ggaatgcgtc
     6001 ccagacgtag tgcgtcgcct gcggcagggc caggagaggc acgagaagga cgagggcgag
     6061 ggcgccgggc tcggccgcgg cgccggggaa gaatcgcggg ttgtcgtgcc acacgagacg
     6121 gtcccagccc cactcctcgg cgaacgcgat cgcgaggagc ggcaggagaa agagcggcag
     6181 gctcgcgacc caccggtccg cggcggcggg aggagccccg gccgcgcggc ggccctcggc
     6241 ccgcgccttc gacgtgaacc agacgagtcc catgtacggg atgccgtgga cgaagacgtt
     6301 cgtgaccgtg aacgcgtagt ccgagtcgag gacgacgatg ccgaggaacc acgtggccac
     6361 ggtcgtcagg acgacgagga ccttgcccca cgaaacgcgc cgtccggcgc gggcgcgggc
     6421 gatctccttg acggcccacg cggcgaggac cgcggcgtac aggacgaacg cggcgcggga
     6481 cacgacgggc ggcaggcccg gaaggaagtc gccctgcagg aaccactgga aacggcgcgg
     6541 caggtgcgcg tgccagaaga ggagcggcgt cagcgtcgcg ccgtagacgg cggccgcgtc
     6601 gaggcggcgg ccccaggagc cttcttcgcc ctcgccgttc ttccggcggt agagcgcgac
     6661 ccagccgtac tgctggcgca cgaagtggaa gacggcgaga tacgcgagaa cccgccagaa
     6721 gaacagggcc gacacggaat acgcggcgac gccggcggcc caggcgagga gcgggacgcc
     6781 gacgtagagc gcgagccgct cgtgggcgtc cgcgccgtcc gcgaggacgc gccaccccgt
     6841 cgaccagacg tgcgcgacgt ccacgccgac gatcgcgagg aggaacagcc acggcggcgc
     6901 gtcgccgtgc aggattccag ccgccatgcc gagcgcgagc agggcgagcg cgagcaccgc
     6961 cgatccgccg aagagcagga gatcggcgcg gcgcgagaag agccagttcg aggtcatggg
     7021 ccggtcgcga tcgtacccga ggccgggcgc ctcacggcct cgccgcgagc gcgcgctcgg
     7081 cgagctgcag ctgcgcgcgg aggtccgcgc ggcccggatc acgagcgagg ccctcgcgcg
     7141 cccaccgcgc cgcgtcctcg aagcggcccg cccacagcag ccgcaggccg cgcagggcgg
     7201 cgg
//
LOCUS       DAOMFY010000203         7170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3345_length_7170_cov_6.985242, whole genome shotgun sequence.
ACCESSION   DAOMFY010000203 DAOMFY010000000
VERSION     DAOMFY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3345_length_7170_cov_6.985242"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLB01_14315"
     CDS             complement(<1..282)
                     /locus_tag="PLB01_14315"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="SAM-dependent;catalyzes the methylation of cytosine
                     at position 1962 of the 23S rRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HQQ78525.1"
                     /translation="MQTLTLARGREAAAKRRHPWLFSGSLARGAPVPGGAVEVRDADG
                     RYVGRGFASPSSPIAARVWTFEDRPLDAAFVAGRLDAAVALRAAVLPPDT"
     gene            352..975
                     /locus_tag="PLB01_14320"
     CDS             352..975
                     /locus_tag="PLB01_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78526.1"
                     /translation="MRENSVIPAHVLAEAGTSDPMLLTKLGVAAVREQKWQKALVLLA
                     EAYRIFAADPELKENMLKIETDAEEPVARIQARGRMFSYYGLALAKSDHSRVQEGARF
                     CEIAIQKEPYQAEHFAHLARIWLTGNNRLKAVQAIDRGLKEVPNSQILLELRGEIGWR
                     ERNAVGFLPRDHPVNVSLGKLLRRKRDRPKSGAPSRPQTPAPPKPKT"
     gene            complement(994..1980)
                     /locus_tag="PLB01_14325"
     CDS             complement(994..1980)
                     /locus_tag="PLB01_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQQ78527.1"
                     /translation="MSFVLFVVFVALAFDFINGFHDAANSIATVVGTRVLTPKMAVVW
                     AAFFNFIAAFSLETRVAKTIGKDIVNPAVVDPLLIFAALVGAIVWNLLTWWLGLPSSS
                     SHALIGGFAGAAVVKAGPGVLVMHGLLKTLVFIVLSPTLGMLLGFLFFLSVTWIFRKA
                     RPEPLDKAFRKVQLVSAAAFSFGHGMNDAQKTMGIIAVLLFSTGHLKSMEIPFWIVLI
                     CHAAIGLGTLSGGWRIVRTMGMRITQLKPVGGSCAEFAGAVTLIGASLGGVPVSTTHT
                     ITGAIMGVGSVRRLSAVRWGVARTIIIAWVLTIPASAFVAALVWLAASAFAR"
     gene            complement(1993..2616)
                     /locus_tag="PLB01_14330"
     CDS             complement(1993..2616)
                     /locus_tag="PLB01_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQQ78528.1"
                     /translation="MIKLFPREETFLPQFVRAADIIVEAARALDALAGDFTDVSLKVA
                     RIKHLEHEGDQVAHQTFGLLSRTWITPIDREDIHALVKALDDVLDFMDAAANRFVLYK
                     IPAPRAGVKQATEILVQSAEAIRRGMGLLGDMKNSPAILEICVEINRLENEADSLNRI
                     SIGALFDTEKDPIALLKWKEIYEVLEEATDRCEDVANVLEQIVLKSS"
     gene            2706..4460
                     /locus_tag="PLB01_14335"
     CDS             2706..4460
                     /locus_tag="PLB01_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQQ78529.1"
                     /translation="MTRRSFGASQVLALALAAPALLAGPAPTPVPAPKPKLAVVISVD
                     GLSYARLESYRPLYTSGLKRLLDEGFALRAARYQHLNTETGPGHASLGTGAPPRVTGI
                     VLNSWFQPGPGGGLVNVYCTDQKDATGKKIAGPDNLRVPTLGDRLVEKTPASRVVSVS
                     AKDRGSIFLAGKNPAHAVYWWDGETGRFQTSAAYRPPAAAAAIVKKYNDTQAGGMLPA
                     RLGLVWDKLPEPAAVPGAKPVPTAVPLNVVSRYQLPVHGLGFPHDLSTYGRIPAGGPY
                     GYFGGIYRSPFVDVLTAELALAFLNDAVLKLGQGDVPDFLAISFSGHDPVAHDYGDES
                     EESRDTLRRLDLQIGRILDALDAAFPKGTVLVALSADHGFPMMPEVARALDKNAPGGR
                     LDTGRDTLGNARDRLNRMLADALCLDRSVKIVGAVDGWSLFYSRGFPYKTVAGACGPA
                     GAPVTPADVDRVLPKIVKSYWEEEIEDVVPVSALAAWPEGPLKTFVRNDFDKERSGDA
                     FVIPRTGVMSTYNPGRGAMHGTHFEYDIHVPLVFWGAVKAGGSDEAATPYDLAPTLGA
                     WLGVALPDAVGKARALPR"
     gene            4457..5623
                     /gene="rsmH"
                     /locus_tag="PLB01_14340"
     CDS             4457..5623
                     /gene="rsmH"
                     /locus_tag="PLB01_14340"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQQ78530.1"
                     /translation="MTDGAPGPPRTRRPRYAGTHPRRFEEKYKERDAARYPEEREKVI
                     ASGKTPAGSHVPVLVAEVLEALAPAPGDVAVDATLGYGGHAAEILKRILPGGRLLALD
                     VDPVELPKATARLGALAAALGAPGAVKTVRRNFAGLRKLLDEEGLARGADVILADLGV
                     SSMQLDDPDRGFSWKHDVPLDLRMNPTRGAPAGALLLKMKREEIEDLLRRLGDEPEAA
                     GIAAALRATIDARGATPPHDANEPPGGSEPHDLLRKSSLPLLRTSDVAAAVESAYKGR
                     TDRDASEAKKSSLQRTFQAIRILANDELTALEAFLRDLPFCLAPGGRAAILTFHSGED
                     RRVKKAFKEGLEAGLYARVADAVVRATPEERGRNPRSSSAKLRWAVRAARSVPR"
     gene            5683..>7170
                     /locus_tag="PLB01_14345"
     CDS             5683..>7170
                     /locus_tag="PLB01_14345"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HQQ78531.1"
                     /translation="MPLLQSPAWNKGTAFTAKERTELGLHGLLPHQIETIDDQLVRAY
                     EAYLAKRTPLGRHIQLRALQDTNEILFYRLLLAHIEEMLPIVYTPVVAEACQQFSHIY
                     RRPRGLFIPYDMRDSIPELLRNRPNPDVDVVVVTDGERILGIGDQGVGGLGIPIGKLT
                     LYTLVGGVPPARTLPIVLDVGTNNEELLRDPQYFGWRQKRVTGEPYFAFVDRFVEAVK
                     AELPGTLLQWEDFATPNARPLLNRYRDSLLTFNDDIQGTAAVTLGALLAATHVAGRSL
                     REQTIVFLGAGSAAIGVADTLRAAMVADGLSDAEARRRFWIVDVDGLLVEGRDGLTPE
                     QRVYARAAEDVAGLPRTRGGAIGLADVIAGVEATVLVGLSTAAGAFTEAIVRAMAAKT
                     PRPVIFPLSNPTARAEARPEDLIRWTDGRALVATGSPCPPAVLGGRAWIISQCNNVFI
                     FPAVGLGVAAAGARRVSDGMLLAAARRLAELSPALTDPAASLLPPL"
BASE COUNT         1143 a         2599 c         2449 g          979 t
ORIGIN      
        1 cgtgtcggga gggagcaccg cggcgcgcag cgcgacggcc gcgtcgagcc gtccggcgac
       61 gaacgccgca tccagcggac ggtcctcgaa cgtccagacg cgcgccgcga tcggcgacga
      121 cggcgacgcg aacccgcgtc ccacgtaccg gccgtccgcg tcccgcacct ccacggcgcc
      181 cccgggcacc ggcgcgccgc gcgcgaggct tcccgaaaag agccacggat ggcggcgctt
      241 cgcggcggcc tcgcgccccc gggcgagcgt cagagtctgc atgccccgac gataccccgg
      301 gggcgacgcc ttgacgccgc gcggcgggga gccgacactt ctgagcacgg cgtgcgtgaa
      361 aacagcgtga tcccggccca cgtgctggcg gaagccggca caagcgaccc gatgctgctc
      421 acgaagctcg gggtcgccgc ggtgcgcgag cagaagtggc aaaaagccct cgtgcttctc
      481 gccgaggcct acaggatctt cgccgccgat cccgaactga aggagaacat gctcaagatc
      541 gagaccgacg ccgaggagcc ggtcgcgcgg atccaggccc gcggccgcat gttctcctac
      601 tacggcctcg cgctcgcgaa gtccgaccac tcgcgcgtcc aggagggcgc ccgattctgc
      661 gagatcgcga tccagaagga gccgtaccag gccgagcact tcgcgcacct cgcacggatc
      721 tggctgacgg gaaacaaccg gctaaaggcg gttcaggcca tcgaccgggg tctcaaagag
      781 gtcccgaaca gccaaatcct cctcgagctg cggggcgaga tcgggtggcg cgagaggaac
      841 gcagtcggat tcctcccccg ggaccacccg gtcaacgtct cgctcggaaa gctgctgcga
      901 cggaagcgcg accggccgaa gagcggggcg ccgtcccgcc cgcagacgcc cgcgccccca
      961 aagccgaaga cctgacccgc gggcggcccc cggtcagcgc gcgaacgccg aagccgcgag
     1021 ccagacgagc gcggccacga aggccgacgc cgggatcgtg agaacccacg cgatgatgat
     1081 cgtccgggcg acgccccacc gcaccgcgga gagccggcgc acggacccga cgcccatgat
     1141 cgcgcccgtg atcgtgtgcg tcgtcgagac ggggacgccg ccgagcgacg ccccgatgag
     1201 cgtgacggcg cccgcgaact cggcgcacga gccgcccacc ggcttgagct gcgtgatccg
     1261 catccccatc gtccggacga tccgccagcc gcccgagagc gtgccgaggc cgatcgccgc
     1321 gtggcagatg aggacgatcc agaacgggat ctccatggac ttcaggtgcc ccgtggagaa
     1381 gagcagcacc gcgatgattc ccatcgtctt ctgcgcgtcg ttcatcccgt gcccgaacga
     1441 gaacgccgcc gccgagacga gctggacctt gcggaaggcc ttgtcgagcg gctcgggccg
     1501 cgccttccgg aaaatccacg tcacggagag aaagaacagg aagccgagga gcatgccgag
     1561 cgtcggcgag aggacgatga agacgagcgt cttgaggaga ccgtgcatga cgaggacgcc
     1621 gggtcccgcc ttcacgaccg ccgcgcccgc gaagccgccg atgagcgcgt gggaggacga
     1681 cgagggcagg cccagccacc acgtcaggag gttccacacg atcgcgccga cgagcgccgc
     1741 gaagatgagt agggggtcca cgacggccgg attcacgatg tccttgccga tcgtcttggc
     1801 gacgcgcgtc tcgagcgaga acgccgcaat aaagttgaag aacgcggccc agacgacggc
     1861 catcttcggc gtcaggacgc gcgtcccgac gacggtcgcg atcgagttcg cggcgtcgtg
     1921 gaagccgttg atgaagtcga aggcgagcgc gacgaagacg acgaaaagga cgaaagacat
     1981 cgtcgtccgg cgctacgagg acttcaggac gatctgctcg aggacgttcg cgacgtcctc
     2041 gcagcggtcg gtcgcttcct cgaggacttc gtagatctcc ttccacttca ggagcgcgat
     2101 cgggtccttc tcggtgtcga acagggcgcc gatcgagatc cgattcaggc tgtcggcctc
     2161 gttctcgagc cggttgatct cgacgcagat ctcgaggatc gccggcgagt tcttcatgtc
     2221 gcctaggagc cccatcccgc ggcggatcgc ctcggccgac tggacgagga tctcggtcgc
     2281 ctgcttgacg ccggcgcgcg gcgccgggat cttgtagagg acgaagcggt tcgccgcggc
     2341 gtccatgaag tcgaggacgt cgtcgagcgc cttgacgagg gcgtggatgt cctcgcggtc
     2401 gatcggcgtg atccacgtgc gcgagaggag cccaaacgtc tggtgcgcga cctggtcgcc
     2461 ctcgtgctcg aggtgcttga tccgggcgac cttgagcgag acgtcggtga agtcgccggc
     2521 gagggcgtcg agcgcccggg cggcctccac gatgatgtcc gccgcgcgaa cgaactgcgg
     2581 gagaaaggtc tcctcgcggg ggaacagctt gatcacgaag cctccgttgc gggcggagac
     2641 taccaaaggt cttaacacgg attttgcgtc tcgtttacac gctgcgcccg tggcaggatc
     2701 ggcgcatgac acgacgctca ttcggcgcct cgcaggtgct cgccctggcc ctggccgctc
     2761 cagccctcct cgcagggccc gcgccgacgc ccgttcccgc gccgaaaccg aagctggcgg
     2821 tcgtgatctc ggtcgacggc ctctcgtacg cgcgcctcga gagctaccgc ccgctctaca
     2881 cgtccgggct gaagcgcctg ctcgacgagg gcttcgccct tcgtgccgcg cgttaccagc
     2941 acctgaacac ggagacgggc ccgggccacg cctccctcgg cacgggcgcg ccgccgcgcg
     3001 tgacgggcat cgtcctgaac agctggttcc aaccgggccc cggcggcggg ctcgtgaacg
     3061 tctactgcac ggaccagaag gacgcgaccg ggaagaagat cgcggggccg gacaacctcc
     3121 gcgtcccgac gctcggcgac cggctcgtcg agaagacccc ggcgtcgcgc gtcgtgtccg
     3181 tctcggccaa ggaccgcggc tcgatcttcc tcgccgggaa gaaccccgcg cacgccgtgt
     3241 actggtggga cggcgagacc ggccgcttcc agacgtctgc ggcctaccgg cctccggccg
     3301 ccgcggcggc gatcgtgaag aagtacaacg acacgcaggc cggggggatg ctgcccgcgc
     3361 gcctcggcct cgtgtgggac aagctccccg agcccgccgc cgttcccggc gcgaagcccg
     3421 tcccgaccgc cgtcccgctg aacgtcgtct cgcgctacca gctccccgtc cacggtctcg
     3481 gtttcccgca cgacctctcg acgtacgggc gcattccggc cggagggcca tacggctact
     3541 tcggcggcat ctaccgcagc cccttcgtcg acgtcctcac cgcggaactc gcgctcgcgt
     3601 tcctgaacga cgccgtgctg aagctcggac aaggcgacgt tccggacttc ctcgcgatct
     3661 ccttctccgg gcacgatccc gtcgcgcacg actacggcga cgagagcgag gagtcgcgcg
     3721 acacgcttcg ccgcctcgac ctccagatcg gccgcattct cgacgcgctc gacgcggcct
     3781 tcccgaaggg gacggtcctc gtcgccctct cggccgacca cggctttccg atgatgcccg
     3841 aggtcgccag ggccctcgac aagaacgccc cgggcgggcg gctcgacacg ggccgcgaca
     3901 cgctcgggaa cgcgcgcgac cgcctgaacc ggatgctcgc cgacgcgctc tgcctcgatc
     3961 gctccgtgaa gatcgtcggc gccgtggacg gctggagcct cttctacagc aggggattcc
     4021 cttacaagac cgtcgccgga gcgtgcgggc ccgcgggcgc gcccgtgacc cccgccgacg
     4081 tcgaccgcgt cctcccgaag atcgtgaaga gctactggga ggaagagatc gaggacgtcg
     4141 tccccgtgtc ggccctcgcc gcgtggcccg aaggtccgct gaagacgttc gtgaggaacg
     4201 acttcgacaa ggagcgctcg ggcgacgcgt tcgtgatccc gcgcaccggc gtcatgagca
     4261 cgtacaaccc cggccgcggc gccatgcacg ggacgcattt cgagtacgac atccacgtgc
     4321 cgctcgtttt ctggggcgcg gtgaaggccg gcggctcaga cgaggccgcg acgccttacg
     4381 acctcgcccc gacgctcggc gcgtggctcg gcgtcgcatt gccggacgcg gtcggcaagg
     4441 cccgcgcgct gccgcggtga cggacggcgc tcccggaccg ccccgaacga ggcggccgcg
     4501 ttacgcgggg acgcacccgc ggcggttcga ggagaagtac aaggagcgcg acgccgcgcg
     4561 gtatccggag gagcgcgaga aggtgatcgc ctcgggcaag acgcccgcgg gaagccacgt
     4621 tcccgtcctc gtcgccgagg tgctcgaggc gctcgcgccg gcgccgggag acgtcgcggt
     4681 ggacgcgacg ctcgggtacg gcggccacgc ggcggagatc ctgaagcgga tcctccccgg
     4741 cggccggctc ctcgcgctgg acgtggaccc ggtcgagctg ccgaaggcga ccgcccgcct
     4801 cggggcgctc gccgcggcac tcggcgcgcc gggcgccgtg aagacggtca ggcgcaactt
     4861 cgcggggctg cggaagctcc tcgacgagga gggtctcgca cgcggcgcgg acgtgatcct
     4921 cgccgatctc ggcgtctcgt ccatgcagct cgacgacccg gacagaggct tctcgtggaa
     4981 gcacgacgtc ccgctcgacc tccggatgaa cccgacgcgg ggcgcgcccg ccggggcatt
     5041 gctgctcaag atgaagagag aagaaatcga agatcttctt agaaggttgg gagacgagcc
     5101 agaagcggcg gggatcgccg ccgcgctgcg ggcgacgatc gatgcgcgcg gggccacgcc
     5161 gccgcacgac gcgaacgaac cgcccggtgg ctccgagcct catgaccttc taagaaaatc
     5221 ttcacttcct cttcttcgaa cctcggacgt ggcggcagcc gtcgagtctg cctacaaggg
     5281 acggacggat cgtgacgcgt ccgaagcaaa gaaatcctct cttcagcgga cgttccaggc
     5341 gatccgtatc ctcgcgaacg acgagctgac cgcgctcgaa gccttcctgc gcgacctccc
     5401 gttctgcctc gcgcccgggg gaagggccgc gattctcacg ttccactccg gcgaggaccg
     5461 gcgagtgaag aaggcgttca aggagggcct cgaggccggg ctctacgcgc gcgtcgcgga
     5521 cgcggtcgtc cgcgcgacgc cggaagagcg gggccggaac ccccgctctt cctccgccaa
     5581 gctgcgctgg gcggtgcggg ccgcccgctc cgttccgcgg taacgtaggg cgcgatgccg
     5641 aactcgaagg ccgcgcaccg gcgccccgac gggctgcgcg gaatgccgct cctccagtcc
     5701 cccgcctgga acaagggcac ggccttcacg gcgaaggagc gcaccgaact cggcctccac
     5761 ggcctcctgc cgcaccagat cgagacgatc gacgaccagc tcgtccgcgc ctacgaggcc
     5821 tatctcgcca agcggacgcc cctcgggcgg cacatccagc tgcgcgcgct ccaggacacg
     5881 aacgagatcc tcttctaccg cctcctcctc gcccacatcg aggagatgct gccgatcgtc
     5941 tacacgcccg tcgtcgccga ggcgtgccag cagttcagcc acatctaccg gcgcccgcgc
     6001 ggcctcttca ttccctacga catgcgcgac tcgattccgg agctgctgcg caaccggccg
     6061 aacccggacg tcgacgtcgt cgtcgtgacg gacggcgagc gcatcctcgg catcggcgac
     6121 cagggtgtcg gcggcctcgg catcccgatc ggcaagctga cgctctacac gctcgtcggc
     6181 ggcgtcccgc cggcgaggac tcttccgatc gtcctcgacg tcgggacgaa caacgaggag
     6241 ctgctccggg acccgcagta cttcggctgg cgccagaagc gcgtcacggg cgagccgtac
     6301 ttcgcgttcg tcgaccgctt cgtcgaggcc gtcaaggccg agctgcccgg aacgctcctc
     6361 cagtgggagg actttgcgac gccgaacgcg cgccctctcc tgaaccgcta ccgcgattcg
     6421 cttctcacgt tcaacgacga catccagggc accgcggccg tcacgctcgg cgcgctcctc
     6481 gcggcgacgc acgtcgcggg aagaagcctg cgcgagcaga cgatcgtctt cctcggggcc
     6541 ggctcggcgg cgatcggtgt cgcggacacg ctccgcgccg cgatggtcgc cgacgggctc
     6601 tccgacgccg aggcccgacg ccggttctgg atcgtggacg tggacggcct cctcgtggaa
     6661 gggcgggacg gcctcacgcc cgagcagcgc gtctacgcgc gcgcggcgga ggacgtcgcg
     6721 ggccttccgc gcacgcgcgg aggcgcgatc ggcctcgcgg acgtgatcgc cggcgtcgag
     6781 gcgacggtcc tcgtcggcct ttccacggcg gccggcgcct tcacggaagc gatcgtccgg
     6841 gcgatggcgg cgaagacgcc ccggcccgtc atcttcccgc tgtcgaaccc gaccgcgcgg
     6901 gcggaggcgc gacccgagga cctcatccgg tggacggacg gccgcgcgct cgtcgccacc
     6961 ggctcgccct gccctcccgc ggtcctcggc gggcgcgcgt ggatcatttc gcagtgcaac
     7021 aacgtcttca tcttcccggc cgtcggcctc ggcgtcgccg ccgcgggagc gcggcgcgtc
     7081 tccgacggga tgctgctcgc cgcggcccgc cgcctcgcgg agctctcgcc ggcgttgacg
     7141 gatcccgccg cgtcgctcct gccgcccctc
//
LOCUS       DAOMFY010000204         7155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3358_length_7155_cov_5.681408, whole genome shotgun sequence.
ACCESSION   DAOMFY010000204 DAOMFY010000000
VERSION     DAOMFY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3358_length_7155_cov_5.681408"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..444)
                     /locus_tag="PLB01_14350"
     CDS             complement(43..444)
                     /locus_tag="PLB01_14350"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HQQ78532.1"
                     /translation="MPPPAPGRFGEPGLTRNEGLSGHPLFEKGLVEFRAGLYFEAHEE
                     WELLWMTAEGDAKLFLQALIQLAAACVHLTRSNAAPGSRLLSLADEKLHRLGGTFGGI
                     NTDSLRKEIGRAQELLGGGASPAEAAKAIRL"
     gene            complement(402..884)
                     /locus_tag="PLB01_14355"
     CDS             complement(402..884)
                     /locus_tag="PLB01_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQQ78533.1"
                     /translation="MKRGTILPCALIALAVGSGCRGREDGASPASAPLDPSKKPLSSS
                     SLPFASLSFDEALEKARAEKRLVFVDVYADWCTWCTKMDEDVFVDPRVQKALLDFVPI
                     KVDTDKSGGRAVANRYRVAGLPAYLVVNGDGELVGRFDGYLPVEAFLLRLRAASGSRG
                     "
     gene            complement(913..1521)
                     /locus_tag="PLB01_14360"
     CDS             complement(913..1521)
                     /locus_tag="PLB01_14360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQQ78534.1"
                     /translation="MARALLLDLGNVLVPFDHGLTLARLEEATGVPASALRPHVFGPL
                     ERDFDLGRLGPAAFFRAVEAAASFPRLSDDVWIPAWRDIFFRDERALGLLRRLAPGAT
                     RVLVSNTNVLHWEGVLAVAPEIPRLLPRRALSFEIGCAKPDPAHFEAALALAGVPPRD
                     AVFADDRAEMVEAARALGIDAFAVARPDDLEEGLRTRSLLGS"
     gene            complement(1523..2770)
                     /locus_tag="PLB01_14365"
     CDS             complement(1523..2770)
                     /locus_tag="PLB01_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78535.1"
                     /translation="MKKLTSTLALVALATSLVACSKPEEKKPEVKAEAPKVVEETKTT
                     SPSTAAAPAETAASASTAAPAAAPATAAAPATKSVAAPLELENAPARRGVSLFWAGTG
                     WQVPCDDRLRRVVMTKRLGTPLALLALLAAASAPAAEPTSSDSRTAPLAPRTFVSIAP
                     RGDTGRTALDSIVTTDAFLPAVHINGASGVYRTDVWIFNPDRDFKAVVDLYYTEADRD
                     GSALPGIRITPDLDPRESVTLSDIVKDYFGKNSSYGLLEVRGSLPLIVTSNTFNVAGA
                     VAGTYGQFSPGQAYRNALGFDNSIFGDLYVTGLVNDPNHRTNAVVMNPSGATLEAGVQ
                     LVDSVGNVYKTVVKVVPPYSMHQLNDIFYQDFASSNPPQGGPWRLNVYVDLSNGAKVL
                     CYATVTDLRTGDPYLIPGQAARP"
     gene            3009..4310
                     /gene="ffh"
                     /locus_tag="PLB01_14370"
     CDS             3009..4310
                     /gene="ffh"
                     /locus_tag="PLB01_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQQ78536.1"
                     /translation="MFESLSNRLQRVFKELRGEGHLTEFHVDEALKEIRTALLEADVN
                     LSVVKDFASAVREKAVGQEVLTSFSPAQQVIKIVRDELTAILGGQQAPLAIKKRPSVI
                     LLVGLQGSGKTTTAAKLALHLKKGFGKQVLLVPADVHRPAATEQLRTLAKENGLMAFD
                     PKGETDAVARARAAVKDAKLFGYDVAIVDTAGRLHVDEVLMDEVARIRAATEPDEILF
                     VADAMTGQDAVRSAEAFGAKLPLTGVILTKTDGDARGGAALSLAKVVGKPLKFTGTGE
                     KVADLEPFHPDRAASRLLGLGDVLTLIEKVSAETDEAAALRAAEKLRKNTFTLTDLRE
                     QMKQLKKMGSLGSLLGHLPKVGPMKQLADMEIPENATKSLEAMIDSMTPAEREDPKIL
                     DAGRKKRIAKGSGTSVPEVNQLLKQYLSMKKMMKGAARMRG"
     gene            4333..4566
                     /gene="rpsP"
                     /locus_tag="PLB01_14375"
     CDS             4333..4566
                     /gene="rpsP"
                     /locus_tag="PLB01_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQQ78537.1"
                     /translation="MLKIRLRRVGGTNAPAYRIVVSDSRRTPTAKVLEELGHYDPTKN
                     PPLLKLDRDKAKVWIAKGASASETVRKLIAKPA"
     gene            4588..5106
                     /gene="rimM"
                     /locus_tag="PLB01_14380"
     CDS             4588..5106
                     /gene="rimM"
                     /locus_tag="PLB01_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQQ78538.1"
                     /translation="MIAPGERIAIGRVIKPHGLKGEVVVEVLTDFPERFSEGLRIFLS
                     GGAREAREARIVAVRPHQGRVLLTFEGISDVSMAETLRGAELSVSAADVAPRPPGFVY
                     HWEIEGAEVFDERGSRLGRVAELQDAAGRPLLVVETPRGLRDVPFAHPIVVTVDMAGK
                     RIVLAPPPGLLD"
     gene            5115..5864
                     /gene="trmD"
                     /locus_tag="PLB01_14385"
     CDS             5115..5864
                     /gene="trmD"
                     /locus_tag="PLB01_14385"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQQ78539.1"
                     /translation="MRFDIVTLFPGFFASPFDESLVGRAVEAGLLDLRVVDLRDFSDD
                     AHRKVDDEPYGGGPGMVLTAPPVFAAVEALRAEAGAPAPHVVFLSPQGRRFDAALAKE
                     LAARPRVALVCGRYEGIDERTRDAGLFDEEISLGDFVLSGGEVAALAVVEAVSRYVPG
                     VVGDAGSVRADSFEDGLLDHPHYSRPREFRGLAVPDVLFSGHHERIRRWRREQQLEAT
                     WRRRPDLLEKAPLSAEERAYLERLAARPRST"
     gene            5954..6304
                     /gene="rplS"
                     /locus_tag="PLB01_14390"
     CDS             5954..6304
                     /gene="rplS"
                     /locus_tag="PLB01_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQQ78540.1"
                     /translation="MSDKLLMAEKAGLRPDVPPFAPGDTVKVHVKVKEGEKERIQVFQ
                     GLVIARKGGGVRETFTVRKISQGVGVERTFPVHSPVLERIEVERHGQVRRAKLYYLRA
                     RRGKAARIAEKRDN"
     gene            6421..>7155
                     /locus_tag="PLB01_14395"
     CDS             6421..>7155
                     /locus_tag="PLB01_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HQQ78541.1"
                     /translation="MFRIEDDARAEGHRLIAGVDEVGRGCLAGPVYAGAVAFEGPEPL
                     LGLDDSKALPPEVREDLAVRIRAAAAGAAVGAATAAEIDALGIVDATLLAMRRALENL
                     ALAGATQPDVEAVAALARTTGGAAQSRLWACGSKVPAAEAAFVNALAASALDYDSLHD
                     NVHPEAIALPVAWAVASAMRASGEEFLRAYILGTEAICRLAESAEGPQKGWTIGAVVG
                     TFGAALTASLLLAHSPDATAHALGLCL"
BASE COUNT         1172 a         2383 c         2523 g         1077 t
ORIGIN      
        1 agattttctt agaaggtgaa gagcgcagcc gacggcgtct ggctacagcc ggatcgcctt
       61 ggcagcctcg gccggtgacg cgcccccgcc cagcagctcc tgcgctcgcc cgatttcctt
      121 tcgaagagaa tctgtattga tccctccgaa tgtccctccc agccgatgga gcttttcgtc
      181 cgcgagggaa agaagacggc tccccggcgc ggcgttgctg cgcgtcaggt ggacgcaggc
      241 ggccgcgagc tggatgaggg cctggaggaa gagcttcgcg tcgccctcgg ccgtcatcca
      301 gagtaattcc cactcctcgt gagcctcgaa gtagagcccg gcgcggaact ccacgaggcc
      361 cttctcgaag agaggatggc cggagaggcc ctcgttcctg gtcagccccg gctccccgaa
      421 gcggcccgga gccggaggag gaatgcctcg accggaaggt agccgtcgaa gcgccccacg
      481 agctctccgt cgccgttcac gacgagatac gcgggcaggc ccgcgacgcg gtatcgattc
      541 gcgacggcgc ggccgccgct cttgtcggtg tccaccttga tcggaacgaa gtcgagaagc
      601 gctttctgga cgcgtgggtc gacgaacacg tcttcgtcca tcttcgtgca ccaggtgcac
      661 cagtccgcat agacgtcgac gaagacgagc ctcttctcgg cgcgcgcctt ttcaagtgcc
      721 tcgtcgaacg agagagaggc gaacgggagg gaagaggaag aaaggggttt cttcgaaggg
      781 tccagaggcg cgctcgcggg cgacgccccg tcctcgcggc cgcggcagcc gcttccgacg
      841 gcaagagcga tcagcgcgca cgggaggatc gttcccctct tcaccctcag agaatatccc
      901 ccggacgacg tctcacgagc cgagaaggct tctcgtgcgc agtccttctt cgagatcgtc
      961 cggcctggcg accgcgaacg cgtcgatccc gagcgcccgc gccgcctcga ccatctcggc
     1021 gcggtcgtct gcgaagacgg cgtcgcgcgg cgggacgccc gcgagggcga gggcggcctc
     1081 gaagtgcgcg gggtcgggct tggcgcagcc gatctcgaac gagagggcgc gcctcggcag
     1141 gagtcggggg atctccgggg ccacggcgag gaccccttcc cagtggagaa cgttcgtgtt
     1201 cgacacgagg acccgcgtcg cgcccggcgc gaggcgccga agcagcccga gagctcgttc
     1261 gtcgcggaag aagatgtccc gccacgccgg aatccagacg tcgtcggaaa gccggggaaa
     1321 cgaagccgcg gcctcgacgg cgcggaaaaa cgcagccggg ccgagccggc cgaggtcgaa
     1381 gtcgcgctcg agaggaccga agacgtgcgg cctcaaagcg gacgcgggga cgcccgtcgc
     1441 ctcctccagc cgcgcgagcg tcagcccgtg gtcgaacggg acgaggacgt tgccgaggtc
     1501 gaggagtagg gcccgcgcca ccctagggtc gcgccgcctg tccggggatg aggtacggat
     1561 cgcccgtcct gagatccgtg acggtcgcgt aacagagcac cttcgcgccg ttggaaaggt
     1621 ccacgtagac gttcagtcgc cacgggccgc cctgcggcgg attcgacgag gcgaagtcct
     1681 ggtagaagat gtcgttgagt tggtgcatcg agtacggggg aaccaccttg acgacggtct
     1741 tgtagacgtt tccaacggaa tccacgagct ggacgccggc ctcgagcgtc gccccggaag
     1801 ggttcatgac gacggcgttc gtccggtggt tcgggtcgtt gacgaggccc gttacgtaga
     1861 ggtcgccgaa gatcgagttg tcgaagccga gcgcgttgcg gtacgcctgc cccggcgaga
     1921 actggccgta ggtgccggcg acggcccccg ccacgttgaa cgtgttgctc gtgacgatga
     1981 gggggaggct gcccctcacc tcgaggaggc cgtagctcga attcttgccg aagtagtcct
     2041 tgacgatgtc cgaaagcgtt accgactcgc gcgggtcgag gtcgggcgtg atgcggatgc
     2101 cgggcaacgc cgatccgtcg cggtccgcct ccgtgtaata gagatccacg accgccttga
     2161 agtccctgtc aggattgaag atccagacgt ccgtccgata gacgccagag gcgccgttga
     2221 tgtggaccgc cggcaggaac gcgtccgtcg tgacgatgga gtcgagggcg gttcggccgg
     2281 tgtcgccccg cggcgcgatg gaaacgaacg tgcggggcgc gagaggcgcc gtcctggaat
     2341 cggaggaggt gggctcggcg gccggcgcgg atgcggcggc gaggagggcg aggagggcga
     2401 gaggcgttcc gaggcgtttc gtcatgacca ctctccggag ccgatcatcg caaggaactt
     2461 gccagcccgt cccggcccaa aaaagagaaa cgccccggcg agccggggcg ttctcgagct
     2521 ccaggggagc agcgactgac ttggtggcgg gggcggcggc cgtcgcaggg gcagcggccg
     2581 gggcggccgt cgaggccgag gccgccgtct cggcgggagc ggcggcggtg gagggggacg
     2641 tcgtcttcgt ctcctcgacg acctttgggg cctcggcctt gacctcgggc ttcttctcct
     2701 cgggcttgga gcaggcgacg agcgacgtgg cgagggcgac gagggcgagg gtggacgtga
     2761 gcttcttcat tttcgggctc tccttgtttt tgattcgttc ttgtcgaacg ttgcgcctta
     2821 tggcgtcttt gtgttctctc gcaagagtaa gggtgatggg gcgtggggag ccgtcgggag
     2881 cgaatacctc aactccgacg gaaaccactc cgcctcgagc ttcgggagca ggataccgca
     2941 tgcagcggtt gtgtcaaccc tgtcagtgag ttggaccggt ttcggcgcct gatacactcc
     3001 gagcggcgat gttcgagtcc ctttccaatc ggttgcagcg cgtcttcaag gaattgcgcg
     3061 gtgaaggcca cctgaccgaa ttccacgtcg acgaagccct caaggagatc cgaacggccc
     3121 ttctcgaggc tgacgtcaac ctgagcgtcg tcaaggactt cgcgtctgcc gtccgggaga
     3181 aggccgtggg acaggaggtc ctgacctcct tctcgcccgc ccagcaggtc atcaagatcg
     3241 tccgcgacga gctgacggcc atcctcgggg gccagcaggc gccgctcgcg atcaagaagc
     3301 gcccttccgt gatcctcctc gtcgggctgc agggctcggg caagacgacc acggccgcca
     3361 agctcgccct ccacctcaag aaggggttcg gcaagcaggt cctcctcgtc ccggccgacg
     3421 tgcatcgccc ggccgcgacc gagcagctcc ggaccctcgc gaaggagaac ggcctcatgg
     3481 cgttcgaccc gaagggggag acggacgccg tggcccgggc gcgggcggcc gtcaaggacg
     3541 cgaagctctt cggctacgac gtggcgatcg tcgacaccgc gggccgcctg cacgtcgacg
     3601 aggtcctcat ggacgaggtc gcacgcatcc gcgccgcgac cgagcccgac gagatcctct
     3661 tcgtcgcgga tgcgatgacc ggccaggacg ccgtgcgttc ggccgaggcc ttcggcgcga
     3721 agctcccgct caccggcgtc atcctcacga agacggacgg cgacgcacgc ggcggcgccg
     3781 cgctctcgct cgcgaaggtc gtcggcaagc ccctgaagtt cacgggcacc ggcgagaagg
     3841 tcgcggacct cgagcccttc cacccggacc gggccgcctc gcgcctcctc ggcctcggcg
     3901 acgtcctgac gctgatcgag aaggtctccg ccgagacgga cgaggccgcg gccctgcgcg
     3961 cggccgagaa actgcggaag aacacgttca cgctgacgga cctgcgcgag cagatgaagc
     4021 agctcaagaa gatgggctcg ctcgggagcc tcctcggcca cctgccgaag gtcgggccca
     4081 tgaaacagct cgcggacatg gagatccccg agaacgcgac gaagagcctc gaggcgatga
     4141 tcgactcgat gacgcccgcc gaacgcgagg acccgaagat cctggacgcg ggccgcaaga
     4201 agcgcatcgc gaagggctcc gggacgagcg tccccgaggt caaccagctc ctcaagcaat
     4261 acctctcgat gaagaagatg atgaagggcg ccgcgcggat gcgcggctga cgtccagaag
     4321 aaaaggaaag acatgctcaa gatccgtctc cgccgcgtcg gcggcacgaa cgcccccgct
     4381 taccgtatcg tcgtctccga cagccggcgc acgccgacgg ccaaggtcct cgaggagctc
     4441 ggccactacg accccaccaa gaaccctccg cttctcaagc tcgaccgcga caaggcgaag
     4501 gtgtggatcg cgaagggcgc ctccgcctcc gagaccgtgc ggaagctcat cgcgaaaccc
     4561 gcctgaggcg ttgcggggag tgatcgcgtg atcgcccctg gcgaacgcat cgcgatcggc
     4621 agagtgatca aaccgcacgg cctcaagggc gaggtcgtcg tcgaggtgtt gacggatttt
     4681 ccggagagat tctcagaagg gttgagaatc tttctctccg gcggcgcgcg cgaggcccga
     4741 gaggcgcgca tcgtggcggt gcgcccgcac cagggccgcg ttcttctcac cttcgaagga
     4801 atctccgacg tttcgatggc cgagaccctt cggggcgcag agctcagcgt gagcgcggcc
     4861 gacgttgctc cccgcccgcc gggattcgtc taccactggg agatcgaggg cgccgaggtc
     4921 ttcgatgaga ggggctcgag gctgggccgc gtggcggagc tgcaggacgc cgccggacga
     4981 ccgcttctcg tcgttgaaac tccccggggt cttcgagacg tgcccttcgc gcacccgatc
     5041 gtcgtcacgg tcgacatggc gggaaaacgc atcgtcctcg cgcctccccc cgggctcctg
     5101 gactagtggc ggccatgcgc ttcgacatcg tcacgctgtt cccgggattc ttcgcctcgc
     5161 ccttcgacga atcgctcgtc ggccgcgcgg tggaggcggg gctcctcgac ctgcgggtcg
     5221 tggacctgcg cgacttcagc gacgacgctc accggaaggt cgacgacgag ccgtacggtg
     5281 gagggcccgg gatggtgctc acggcgccgc cggtcttcgc ggccgtcgag gccctgaggg
     5341 cggaagcggg tgcaccggcc ccgcacgtcg tgttcctctc tccgcagggc cggaggttcg
     5401 acgccgccct cgcgaaggag ctcgccgcgc gcccgagggt cgcgctcgtg tgcgggcgct
     5461 acgagggcat cgacgagcgc acccgggacg ccgggctctt cgacgaggag atctctctcg
     5521 gcgacttcgt cctctcgggc ggggaggtcg cggccctcgc ggtcgtcgag gccgtgtcgc
     5581 ggtacgtgcc gggcgtcgtc ggcgacgccg gcagcgtccg ggcggacagc ttcgaggacg
     5641 gactcctgga tcacccgcac tacagccggc cgcgcgagtt ccgcgggctg gccgtgccgg
     5701 acgtcctctt ctcgggtcac cacgagagga tccggcgctg gaggcgcgag cagcagctcg
     5761 aggccacgtg gcggcgccga cccgacctcc tcgagaaagc ccccctctcc gccgaggaaa
     5821 gggcctacct tgagcgactt gcggcccggc cgcgatccac gtagaatcag cggctacggg
     5881 cgaactttcc ggggtgcgga tgtacgtgtc cctgcctgat tccccccgtc agcaaggtat
     5941 tggaggttcc gcgatgtcgg acaagcttct gatggccgag aaggccggac tcaggccgga
     6001 cgttcccccg ttcgcgcccg gcgacacggt caaggtccac gtcaaggtga aggaaggcga
     6061 aaaggaacgc atccaggtct tccagggcct cgtcatcgcg cgcaagggcg gcggcgtgag
     6121 agagacgttc acggttcgca agatctccca gggtgtcggc gtggagcgca cgttccccgt
     6181 gcactctccg gtcctcgagc gcatcgaagt ggagcgccac ggacaggtcc gccgcgcgaa
     6241 gctctactat ctccgcgcgc gccgcggcaa ggccgcccgc atcgcggaga agcgcgacaa
     6301 ctagtctcca cactctccgt cgcgggccgt cccggggcgg ggtacagttg aggcacgatg
     6361 ggacctggcg ccggccggaa tgcatgcgac ctggggtctg agaggaaacg tgtctctgcg
     6421 atgttccgca tcgaggacga cgcgcgcgcc gagggccacc gtctgatcgc cggcgtggac
     6481 gaggtcggtc ggggctgcct cgcaggaccc gtgtacgcgg gtgccgtcgc gttcgagggt
     6541 cccgaaccgc tcctcggcct cgacgactcc aaggcgctcc cgcccgaggt tcgcgaggac
     6601 ctcgccgtcc gcatccgcgc cgccgcggcg ggagccgccg tcggcgcggc gaccgccgcg
     6661 gagatcgacg cgctcggcat cgtcgacgcg acgctcctcg cgatgcgccg ggccctggag
     6721 aacctcgcgc ttgccggcgc gacgcaaccc gacgtcgagg ccgtcgcggc actcgcgcga
     6781 accaccggcg gcgcggcgca gagccgcctc tgggcctgcg gttcgaaggt gccggccgcg
     6841 gaagccgcat tcgttaacgc gctcgccgcc tcggcgctcg actacgacag cctgcacgac
     6901 aacgtgcacc cggaggcgat cgcgctgccc gtggcctggg cggtcgcgag cgcgatgcgc
     6961 gcgagcggcg aggaattcct gcgcgcctac atactcggca cggaagcgat ctgccgcctc
     7021 gccgagtcgg cggaagggcc gcagaagggg tggacgatcg gcgcggtggt cggcacgttc
     7081 ggcgcagcgc tcaccgcttc gctgctgctc gcgcactcgc ccgatgcgac ggcgcacgcg
     7141 ctgggcctgt gcctg
//
LOCUS       DAOMFY010000205         7084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3411_length_7084_cov_6.773510, whole genome shotgun sequence.
ACCESSION   DAOMFY010000205 DAOMFY010000000
VERSION     DAOMFY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3411_length_7084_cov_6.773510"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..572)
                     /locus_tag="PLB01_14400"
     CDS             complement(72..572)
                     /locus_tag="PLB01_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78542.1"
                     /translation="MTVGLARLPGGPVVLRARAPLDAPADRVTAILEDVARWPTWIRR
                     LRSFELLSGNPPAYAAVFAAPWPLKEREYALAPAVARDRDGAVVFWEDASMRLAPPAS
                     GRVRVAPVSGCFAVSPGPAPGGATLVYTESDVFGESLPAWMRGAGRAKGPVRLLDGLR
                     ARLREN"
     gene            complement(749..2035)
                     /locus_tag="PLB01_14405"
     CDS             complement(749..2035)
                     /locus_tag="PLB01_14405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ78543.1"
                     /translation="MSRLPVFPDAALRERALAEAWERLDPRFRLANQVLGRRSTVGCV
                     ALEITQRCNLDCTLCYLSESSESVKDTPLEILFERIDRIRAQYGAGVSVQVTGGDPTL
                     RDRAELVAIVRRVAERDLRPSLFTNGIKATRDLLEELAAAGLYDVAFHVDTTQRRRRF
                     PTEESLHAIRREYIERARGLGIAVIFNTSVHGGNLDEIPGLVRFFLAHSDVVGMASFQ
                     VQAATGRGEWRARAPEVTMRAIRERVTAAVGEGGLAWNAALLGHPDCHEATMLATFGG
                     RAVDVLADQTLYESFLEEMKHVPFDRRDVPGTARQVLLALARRPWWVLRGGAFVLSRL
                     WRVRRELLARRKTGKITFFIQNFMDADALDEERLAHCSFMVMTDDGPVSMCAHNARRD
                     DYILKPLALPSAGGAGVWDPKTGALALPSLPAARAS"
     gene            2266..2952
                     /locus_tag="PLB01_14410"
     CDS             2266..2952
                     /locus_tag="PLB01_14410"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HQQ78544.1"
                     /translation="MLSAFVDDRGTVDYAALARAPQALDRFLAAVERTSPDVEPALFP
                     TRNDQLAYYLNAYNAWVFRGVLARGPERESVWKGGLVSGYSFFVGMDIVLGGVKTNLK
                     KLEDKTVRARFEDPRIHAALNCASRGCPRLPRKAFEPTTLDAELDAAMREFVSEERNV
                     SVDTAARRLTLSKIFDWFEGDFLAFERKQGNASPKLADYVNRYRAADATVPRDFRVRF
                     AEYDKGLNGR"
     gene            2981..3802
                     /locus_tag="PLB01_14415"
     CDS             2981..3802
                     /locus_tag="PLB01_14415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HQQ78545.1"
                     /translation="MPPRALSSPVLAVLDVGFFRHPDLTSRRVLACVDATGRRVVPFH
                     YGAPGTGAEHGTRTMLLAAGSGRASGLVSAAPRSKVVLVKVGEGGRVPRAAIVRAFRW
                     LLAHGGRWGIRVVLCPFGDDPEIRGEKSEVPALVAALAARGLVVVAAAGWDPANEVIS
                     PARSPHAIAVGGWDLADNRPARGPRVGLVEGVLKPDLLAPAVPLEVPCLQGSRTHERA
                     GGTSFAASLVAGAALRILGKEPDLGKDGVLARLAALGRPVPGHPPFLPLRVFRRT"
     gene            3902..4381
                     /locus_tag="PLB01_14420"
     CDS             3902..4381
                     /locus_tag="PLB01_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQQ78546.1"
                     /translation="MSGNTDERRVFDEFLKRKGLKVTRERTAVFDEIFSTHRHFDADD
                     LVSRMRERGTKISRATIYRTLELLYGCGLVGRVRLNEEKYRYERLRKGEHHDHLVCTN
                     CGKVVEFVDGAIEKRQDAVCKEHDFVATSHSHQIWGLCGACRKVAMKAPGRAPSTSA"
     gene            4392..5831
                     /locus_tag="PLB01_14425"
     CDS             4392..5831
                     /locus_tag="PLB01_14425"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQQ78547.1"
                     /translation="MPVAVVWLRRNLRLDDNRVLDAALRAADAVVPVFVLDDHYLEDD
                     YSPPRLAFLAASLSELAAALQAKGGRLVVRNGPAGDALAALCRETGADAVFTHADHEP
                     HGKALHDEARRTLAAQGTSFHAVEDLLLLPPGRLATQEGKPFTVYTPFSRRWLEADKP
                     SPVPEPVRVPAPDAVLSAAFPSIPLEKPRGFRETGAPANPEAGAAAARRTWDAFRASA
                     LFRYAAERDRPDLEGTSRLSPHLRFGTIGIRRVLAEARAAWKDAPAEGRKGIETFVKE
                     LAWREFYAGILHAFPRVLTENFRKDFDAFPWVSGEEEARRFAAWAEGRTGYPIVDAGM
                     RQLLAEGWMHNRVRMIVASFLTKDLLIDWRRGEAFFRRHLADGDPASNNGGWQWAAGC
                     GTDAQPFFRIFNPVLQGTKFDPAAAYVTRWIPELAHVAAKPAELHAPWTLTSPPADYP
                     APVVDHAAARAAALAAFATLAAFATLKKG"
     gene            complement(5844..>7084)
                     /locus_tag="PLB01_14430"
     CDS             complement(5844..>7084)
                     /locus_tag="PLB01_14430"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQQ78548.1"
                     /translation="PALTPAQRAEAERLLAGLSTLLAQPLADDGRRRLLENGVRLARR
                     AGRNDDARTLLLRLLEIDPVNDAGASEAFRDAFDLYTAGRYAEAARAFEEQSALWREV
                     GVKRRATYWAARSKERLGDAAGAQARYANLVSSAAPDLYARWAASALGIGLAVPVPAF
                     ADDASAQSETPLAPSRELLLCGFPDLAGDAAELEGTLDPVFAARVAAGTGDYRRAAGL
                     LKRQYPELGTPEEGAVPAEARRAYYPLAHAGIVEREAQRVGLPASLLYGVIRQESVFT
                     ADIRSKAGALGLMQVMPATGKSMNRRENGSKGRPDLRDPAENVHLGAAYLRDLLAEFQ
                     GDTAAAVAAYNAGPGRVRSWKRAAGGAPMDEFLEGIPITETRVYVKRVLYFQSAYAAL
                     YGLPLDAPVPRLEAPAPAAP"
BASE COUNT          990 a         2524 c         2589 g          981 t
ORIGIN      
        1 cgggcgcggc ccggggcggc cgcgagaacg ggcgtcgcca gcgcgagaag gagagcggcg
       61 gcgcgcacgc gtcaattctc gcgcagccgg gcgcgcaagc cgtcgaggag gcggaccggg
      121 cccttcgcgc ggccggcgcc gcgcatccac gcgggcaggc tttcgccgaa gacgtcgctt
      181 tccgtgtaca cgagcgtcgc gccgcccggc gcgggccccg gcgacaccgc gaagcacccg
      241 ctcacgggag ccacgcggac gcggccggaa gccggcggcg cgaggcgcat cgaggcgtcc
      301 tcccagaaca cgacggcccc gtcgcggtcg cgggcgacgg cgggtgcgag ggcgtactcg
      361 cgctccttca gcggccaggg ggccgcgaac accgccgcgt aggccggagg gttgccggag
      421 agaagctcga aggaacgcag gcgcctgatc cacgtaggcc agcgtgcgac gtcttcgagg
      481 atcgcggtga cccggtccgc cggggcgtcg agaggggcgc gcgcgcgcag gaccacgggg
      541 ccgcccggca ggcgcgcgag gccgacggtc acgccatgct cctcgttcag cggcgagaag
      601 agcgcgtccg ggagacggca ggggtgcgcc ggagggagct cccgcttcgg cggcgtcgag
      661 gcccgcggac cgtccgcggc cacggaggcg gggagagaag ccgcgagaag aacggcaaga
      721 agaaaagaca ctgcgccccc gcgcgggatc acgaggcgcg cgccgcgggg agggacggca
      781 gcgcgagcgc gcccgtcttc gggtcccaga ccccggcgcc gcccgcggac gggagggcga
      841 gcggcttcag gatgtagtcg tcgcgccgcg cgttgtgcgc gcacatcgag acggggccgt
      901 cgtccgtcat gaccatgaac gagcagtgtg cgaggcgctc ctcgtcgagc gcgtccgcgt
      961 ccatgaagtt ctggatgaag aacgtgatct tgccggtctt cctccgcgcg agaagctcgc
     1021 gcctcacccg ccagaggcgc gagaggacga acgcgccccc gcgcaggacc caccagggcc
     1081 tgcgggcgag cgcgaggagg acctgccgcg ccgtgcccgg gacgtcgcgg cggtcgaacg
     1141 gcacatgctt catttcctcg aggaagctct cgtagagggt ctggtcggcg aggacgtcca
     1201 cggcgcggcc gccgaacgtc gcgagcatcg tggcctcgtg gcagtccggg tgcccgagga
     1261 gcgcggcatt ccacgcgagg cccccttccc cgaccgccgc ggtgacgcgc tcgcggatcg
     1321 cccgcatcgt gacctcgggt gcgcgcgcgc gccactcgcc gcggcccgtc gccgcctgca
     1381 cctggaacga ggccatcccg acgacgtcgg agtgggcgag gaagaagcgg acgagccccg
     1441 ggatctcgtc gaggttcccg ccgtggacgg acgtgttgaa gatcacggcg atgccgaggc
     1501 cgcgggcgcg ttcgatgtac tcgcggcgga tcgcgtggag cgactcctcg gtgggaaagc
     1561 gcctgcgccg ctgcgtcgtg tccacgtgga acgcgacgtc gtagaggccg gccgccgcga
     1621 gctcctcgag gaggtcccgc gtggccttga tgccgttcgt gaacagcgag gggcggaggt
     1681 cgcgctcggc gacgcgcctc acgatcgcga cgagctccgc gcggtcgcgc agcgtcgggt
     1741 ccccgcccgt gacctgcacg ctcacgcccg ccccgtattg cgcgcggatg cggtcgatgc
     1801 gctcgaagag gatctcgagc ggcgtgtctt tcacggattc ggaggattcc gagagatagc
     1861 agagcgtaca gtcgaggttg cagcgctgcg tgatctcgag ggcgacgcac ccgaccgtcg
     1921 agcgccgccc gagcacctgg ttcgcgaggc ggaaacgcgg gtcgagcctc tcccacgcct
     1981 cggcaagcgc gcgctcgcgg agggcggcgt cgggaaaaac gggcagacgg ctcaccggat
     2041 ggaatattcg caaggggatg gaagatattc cctcggacgg gagtacgagc gacgggtaag
     2101 gggggatttc ttcgaatgag aaagagcggg catcggggcg ccgcaggcat gctgccggct
     2161 ttcctccggc tgacgtcagc tttcgtgatc ttgagcggcc ttgcgctcga tgcttcgccc
     2221 gcgccagcgc ccgatgcccc gttctcctat gcagactgga atctcgtgct ttcggccttc
     2281 gtcgacgacc gtggcacggt cgactacgcg gcgctggccc gcgcccctca ggccctcgac
     2341 cggtttctcg cggcggtgga gcggacgagc ccggacgtgg agccggcgct ctttccgacc
     2401 cggaacgacc agcttgccta ttacctcaac gcctacaacg cgtgggtctt tcgcggggtc
     2461 ctcgcccgcg ggccggagag agaaagcgtc tggaaggggg ggctcgtctc cggctactcg
     2521 ttcttcgtcg ggatggacat cgtcctcgga ggcgtgaaga cgaacctcaa gaagctcgag
     2581 gacaagacgg tgcgcgcccg cttcgaggac ccgcgcatcc acgccgcgct gaactgcgcg
     2641 agccgggggt gcccgcgcct gccgcgaaag gccttcgagc ccaccacgct cgacgccgag
     2701 ttggatgccg cgatgaggga gttcgtctcc gaggaacgga acgtctcggt cgacacggcc
     2761 gcccgccgcc tgacgctctc gaagatcttc gactggttcg agggcgactt cctcgccttc
     2821 gagagaaagc agggcaacgc gagcccgaaa ctcgccgact acgtgaaccg gtaccgcgcc
     2881 gcggacgcga cggtgccccg ggacttccgg gtccggttcg cggaatacga caaggggctc
     2941 aacgggcgct gaagggtcgc agcccgtaca ctccgcggcc gtgccgccgc gagcgctttc
     3001 ctctcctgtc ctcgccgtcc tcgacgtcgg cttcttccgc cacccggacc tgacctcgcg
     3061 tcgcgtcctc gcgtgcgtcg acgcgacggg ccggcgcgtc gtcccgtttc actacggggc
     3121 gccgggcacc ggggccgagc acggcacgcg gacgatgctc ctcgcggcgg gcagcggccg
     3181 cgcgagcggg ctcgtctcgg ccgccccgcg ttcgaaggtc gtcctcgtga aagtcggcga
     3241 gggaggccgg gtgccgcgcg ccgcgatcgt gcgcgcgttt cgctggctcc tcgcgcacgg
     3301 aggacgatgg ggcatccgcg tcgtcctctg tcccttcggc gacgatcccg agatccgggg
     3361 cgagaagagc gaggtgccgg cgctcgtcgc ggctctcgcc gcccgcggcc tcgtcgtcgt
     3421 cgccgccgcg ggctgggatc cggcgaacga agtcatctcg ccggcgcgct cgccgcacgc
     3481 gatcgcggtc ggcggctggg acctcgcgga caaccgtccc gcgcgcgggc cgcgggtcgg
     3541 cctcgtcgag ggcgtgctga agccggatct cctcgcgccg gcggtcccgc tcgaggtgcc
     3601 gtgcctgcag ggcagccgga cacacgagcg ggccggggga acgtcgttcg cggcctccct
     3661 cgtggcgggg gccgcgctcc gcattctcgg aaaggagccg gacctcggga aagacggtgt
     3721 cctcgcgcgt ctcgcggccc tcgggcgccc cgtccccgga caccccccgt tcctgcccct
     3781 ccgggtgttc cggcggacct gacccccccg gatttcttga ggttttcggg gttccgcgtc
     3841 tggactcaga tgcaactgca ccattccttt ccgttttccg gaggggcatc gtcccctcac
     3901 catgagcgga aacaccgacg aacgccgcgt cttcgacgaa ttcctcaagc gcaagggact
     3961 caaggtcacg cgcgagagaa cggccgtgtt cgacgagatc ttctcgacgc accggcactt
     4021 cgacgccgac gacctcgtga gccgcatgcg cgagcggggc accaagatca gccgggcgac
     4081 gatctaccgc acgctcgagc tgctctacgg gtgcggcctc gtgggtcgcg tccgcctgaa
     4141 cgaggagaag taccgctacg agcggctccg caagggcgag caccacgacc acctcgtctg
     4201 cacgaactgc ggcaaggtcg tggagttcgt cgacggcgcc atcgagaagc ggcaggacgc
     4261 ggtctgcaag gagcacgact tcgtcgcgac gtcgcactca caccagatct ggggtctctg
     4321 cggcgcctgc cggaaggtcg ccatgaaggc tccgggccgg gctccttcga cctccgcgta
     4381 gactcgggcg cgtgcccgtc gccgtcgtct ggctgcggcg gaacctccgc ctcgacgaca
     4441 accgcgtcct cgacgcggcc ctccgggccg cggacgcggt tgtcccggtc ttcgtcctcg
     4501 acgaccacta cctcgaagac gactactcgc cgcctcgcct cgccttcctc gccgcgtctc
     4561 tgagcgagct cgcggccgcc ctgcaggcga aggggggccg cctcgtcgtc cgcaacggtc
     4621 cggcgggcga cgcgctcgcg gcgctctgcc gcgagaccgg cgcggacgcg gtcttcacgc
     4681 acgcggacca cgagccgcat ggaaaggctc ttcacgacga agcgcgccgc accctcgcgg
     4741 cgcaggggac ctcgtttcat gccgtcgagg acctgctcct ccttccgccg gggcggctcg
     4801 cgacgcagga aggcaagcct ttcacggtgt acacgccgtt ctcgcgccgc tggctcgagg
     4861 cggacaaacc ttcccccgtt cccgagcccg tccgcgttcc ggcgccggac gcggttctct
     4921 ccgccgcgtt cccgtccatc ccgctcgaga agccgcgcgg cttccgcgaa acgggcgcac
     4981 ccgcgaaccc ggaggccggc gcggctgcgg cgcgccggac gtgggacgcg ttccgcgcgt
     5041 cggcgctctt ccggtacgcc gcggagcgcg accggccgga cctcgaagga acgtcgcgac
     5101 tctctccgca tctccgcttc gggacgatcg ggatccgccg cgtcctcgcc gaggcgcgcg
     5161 ccgcgtggaa ggatgcgccg gccgaaggcc ggaaggggat cgagacgttc gtgaaggagc
     5221 tcgcctggcg cgagttctac gccgggatcc tccacgcgtt cccgcgcgtc ctcacggaga
     5281 acttccggaa ggacttcgac gcgttccctt gggtttccgg cgaggaggaa gcgcggcgct
     5341 tcgcggcctg ggcggagggg cgcacggggt atccgatcgt cgacgcgggg atgcggcagc
     5401 ttctcgccga aggctggatg cacaaccgcg tgcgcatgat cgtggcgtct ttcctcacga
     5461 aggacctcct catcgactgg cggcggggcg aggcgttttt ccgccgtcat ctcgccgacg
     5521 gcgaccccgc gtcgaacaac gggggctggc agtgggccgc cgggtgcggg accgacgcgc
     5581 agccgttctt ccggatcttc aaccccgtcc tgcaggggac gaagttcgat cccgccgccg
     5641 cgtacgtgac gcggtggatc cccgagctcg cgcatgtcgc ggcgaaaccc gccgagctcc
     5701 acgcgccgtg gaccctgaca tccccgccgg ccgactatcc cgcccccgtg gtcgaccacg
     5761 ccgcggcgcg ggccgcggcg ctcgcggcgt tcgcgacgct cgccgcgttc gcgacgctga
     5821 agaaaggctg aagaacctcc cgcttacgga gccgcgggtg cgggggcctc gaggcgcggc
     5881 acgggcgcgt cgagcggcag cccgtagagc gcggcgtacg cgctctggaa gtagagcacg
     5941 cgcttgacgt agacgcgcgt ctccgtgatc gggatccctt cgaggaactc gtccatcggc
     6001 gcgccgcccg cggccctctt ccacgacctc acgcgtcccg gccccgcgtt gtacgcggcg
     6061 acggcggcgg ccgtgtctcc ctggaactcc gcgagcaggt cgcgcagata cgcggcgccg
     6121 aggtgcacgt tctcggcggg gtcccggagg tccggccggc ccttcgaacc gttctcgcgc
     6181 cggttcatcg acttcccggt cgcgggcatg acctgcatga ggccgagcgc accggccttc
     6241 gagcggatgt ccgcggtgaa gacgctctcc tgccggatga cgccgtagag gagcgaggcg
     6301 ggaaggccga cgcgctgcgc ttcacgctcg acgatgccgg cgtgcgcgag cgggtagtac
     6361 gcgcgcctcg cctcggcggg aaccgcacct tcctccggcg ttccgagctc cgggtactgc
     6421 cgcttgagga gccccgcggc gcggcggtag tcacccgtgc ccgcggcgac ccgcgccgcg
     6481 aacacgggat cgagcgttcc ctcgagctcg gcggcgtcgc ccgcgaggtc cgggaagccg
     6541 cagagcagga gctcgcgcga cggggcgagc ggggtctcgc tctgcgcgga ggcgtcgtcg
     6601 gcgaaggcgg gaacggggac ggcgaggcct atgccgagcg ccgacgccgc ccagcgcgcg
     6661 tagagatcgg gggcggcgct ggagacgagg tttgcgtagc gcgcctgggc gcctgccgcg
     6721 tcgccgagcc gctccttcga ccgcgcggcc cagtacgtcg cgcgcctctt gacgccgacc
     6781 tcacgccaga gcgcgctctg ctcctcgaag gcgcgcgcgg cctcggcgta ccgtcccgcc
     6841 gtgtagaggt cgaacgcgtc gcggaacgcc tcggaggcgc cggcatcgtt cacgggatcg
     6901 atctcgagga ggcgcagcag gagcgtgcgc gcgtcgtcgt tccggcccgc gcggcgggcg
     6961 aggcgcacgc cgttctcgag aaggcggcgt cggccgtcgt ccgcgagcgg ctgcgcgaga
     7021 agcgtcgaga ggccggcgag gaggcgctcc gcctcggccc gctgggcggg cgtgagcgcg
     7081 ggcg
//
LOCUS       DAOMFY010000206         7064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3429_length_7064_cov_6.817806, whole genome shotgun sequence.
ACCESSION   DAOMFY010000206 DAOMFY010000000
VERSION     DAOMFY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3429_length_7064_cov_6.817806"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLB01_14435"
     CDS             <1..650
                     /locus_tag="PLB01_14435"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQQ78549.1"
                     /translation="AAFERETSNQVLFWVDRRVPEGTTLEEFANRCFREWGVGQKGKS
                     NGVVFFYFTEDHRMRMEVGYGLEGAIPDARAHRIDAEIIAPRFRAGDVAGGLEAGADA
                     IFAAARGEPYEGTGKTAAETDGGRRTRTFVRVALLSAFALGFGFLIVVTWLRFRRRRA
                     PGSRLATSSSEPSSSSGWSSDSSSSSSSSSSDSSSSSDFSGGGGDSGGGGSSDSD"
     gene            656..1411
                     /locus_tag="PLB01_14440"
     CDS             656..1411
                     /locus_tag="PLB01_14440"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQQ78550.1"
                     /translation="MRLAAVLALSLSAAPAAVSAAGPSVPPAPTRFVTDKAGALSPDT
                     AARLEQRLESFEKETSNQFLVYTERVVPDGTTLEEYTVACAQAWKAGQTQRKNGMILF
                     VFPGSRKVRLEVGYGLEGAMPDALARRVLDEQVLPRFRTGDYDGGVSAGVEAAIAATK
                     GEYKGSGTTQAKKKGRGEIPIPFPVFLLLLFLFFFVILPAARGGRRGKYWYLSSGGFG
                     GGGFGGGGFGGGGGGGFSGGGGSFGGGGASGDW"
     gene            1411..1857
                     /locus_tag="PLB01_14445"
     CDS             1411..1857
                     /locus_tag="PLB01_14445"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQQ78551.1"
                     /translation="MGEKPAKAFFESLDHPAIVEAIRRAEARSFGEIRVHLHHGSVAD
                     ARAEAEKTFLRLGMDKTARGSGCLIFLAPESRAFAVIGGAGIHEKVGDGFWLEARDAA
                     RERFTEGKFTEGIVAAVEKLGEALALFFPRDGTSDRNELPDDVSED"
     gene            1863..3665
                     /locus_tag="PLB01_14450"
     CDS             1863..3665
                     /locus_tag="PLB01_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HQQ78552.1"
                     /translation="MKRVTLPFCALLLLAGTARAQTAPPHPTADDAVRFVEAAEKRLL
                     ALTVEASRAGWVQANFITQDTEALSALASERLISATAELAKQAETFRGVPVPPETARK
                     LEKIRLSLDLAAPADPAKSAELTRLATGLDAAYGRGRACLPGAKECLTFEDVNRRMAA
                     SRDPQELLALWKAWHAVGTPLRADYARFVTLANEGAKELGFADTGAMWRSKYDMPPDA
                     FAKELDRLWDEVKPLYVALHTYVRTKLRETYGDAVPSKGPIPAHLLGNMWAQDWTNVY
                     PLLAPKSADPGYDLTKILEGRKVDAKGMVKYGEGFFTSLGFAPLPATFWERSLFTRPR
                     DRDVVCHASAWDVDSESDIRIKMCTEPTAEDFVTVHHELGHNFYQRAYAKQPPLFRES
                     ANDGFHEAIGDAVALSITPKYLVRLGLVEKEPPPAGDIGFLLNRALEKVAFLPFGLLI
                     DQWRWRVFAGEVTPANYNAAWWELKRKIQGVVPPEVRSEADFDPGAKYHVPGNVPYMR
                     YFLAHVLQFQFHRALAQAAGCAEPLHRCSIYGSKEAGRRLNEMLEMGSSRPWPEALEK
                     LTGQRKIDATALLDYFAPLRKWLDEQNAGKPSGW"
     gene            3766..4086
                     /gene="rplU"
                     /locus_tag="PLB01_14455"
     CDS             3766..4086
                     /gene="rplU"
                     /locus_tag="PLB01_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQQ78553.1"
                     /translation="MYAVIRTGGKQVRVAEGDVVRVERLTKETLKKGAEVSLGEVLAI
                     GREDGLHLGSPLVEGASVTAVVVRETRGPKLLVYKKKRRKGFQRTHGHRQDLVEVRIA
                     SIKA"
     gene            4102..4371
                     /gene="rpmA"
                     /locus_tag="PLB01_14460"
     CDS             4102..4371
                     /gene="rpmA"
                     /locus_tag="PLB01_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQQ78554.1"
                     /translation="MAHKKGQGSSRNGRDSNAQRLGVKRFSGQLVTGGSILVRQRGTP
                     LKPGLNVGRAKDDSLFAKVDGVVNFHDKGRLGRFVSITPVEQKPN"
     gene            4375..5430
                     /gene="obgE"
                     /locus_tag="PLB01_14465"
     CDS             4375..5430
                     /gene="obgE"
                     /locus_tag="PLB01_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQQ78555.1"
                     /translation="MFIDEAAIHVAGGDGGNGCVAFRREKFIPHGGPSGGDGGDGGSV
                     TLVADPSLNTLYPFRFTKRFVAGRGGHGRGNNCHGKNGRPLEVSVPVGTVVKDRESGE
                     ILGDLVAQGQTLLIAPGGRGGRGNARFATSTNRTPRRADPGTEGVTRELALELKLLAD
                     VALVGLPNAGKSTLIAAISAARPKIADYPFTTLVPNLGVVSWDRFRTFVAADVPGLIA
                     GAHEGHGLGIRFLKHVERSRILAHLVDAASLSAEDPAGDAEAAVATIENELTAFQPEL
                     ARRERVLVATKADAATPEQIEAVSRAARRRGLTFFAISAAARQGLEPLVAHLGTRLDA
                     LAALSTPVPVPGSPEAP"
     gene            5427..6077
                     /gene="nadD"
                     /locus_tag="PLB01_14470"
     CDS             5427..6077
                     /gene="nadD"
                     /locus_tag="PLB01_14470"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQQ78556.1"
                     /translation="MTLRIGVYGGTFDPVHVGHVFPVEETRVNLGLDRVLYVPASQPP
                     HKPASPSAPAHHRFAMLALALEPYPNLLLSDFEVARGGTTYTIETLRHLRAQREDAEI
                     LLVLGSDSLAQIETWRSFREILAEFRLAVVAREGFTREALFGALPKDVAGRFAPEGSR
                     PGGDVPEQTIYWGGNAPVTISSTWLRRAIPAGEDLSGSLPENVAAYLRKHALYRLP"
     gene            6074..6535
                     /gene="rsfS"
                     /locus_tag="PLB01_14475"
     CDS             6074..6535
                     /gene="rsfS"
                     /locus_tag="PLB01_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQQ78557.1"
                     /translation="MKHPVPPIGDVDDLIAATLARATGPAGERPQRPADAELDARLTA
                     HLKRGVAALLDKKAEKLVVLNLQGLTAMADYFVLATASSDRQAQALADAVEAALKAEG
                     RRPLSIEGYSSPWILVDYGDVVFHIFHDEARRFYGLERLWGDAPDATASFR"
     gene            6541..>7064
                     /locus_tag="PLB01_14480"
     CDS             6541..>7064
                     /locus_tag="PLB01_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HQQ78558.1"
                     /translation="MPARTRAAAPAPLFFATRHRGLREQLRRLARAADSANTVLLLGA
                     PGTGKDRLARLLHERSRRAAEPFVRVDLAAIADDLFESELFGHERGAFTGAVSPKPGL
                     LEGAGAGTLYLDRIDALSLRAQGKLLRVVEERLVRRVGGTRQTALRARLVASAGPDLP
                     RRARSGEFREDLFYR"
BASE COUNT         1040 a         2544 c         2460 g         1020 t
ORIGIN      
        1 tcgccgcgtt cgagcgcgag acgtcgaacc aggtcctctt ctgggtcgac cgcagggtgc
       61 ccgagggcac gacgctcgag gagttcgcga accgttgctt ccgcgaatgg ggcgtcggcc
      121 agaagggcaa gtcgaacggc gttgtcttct tctacttcac ggaagaccac cggatgcgga
      181 tggaggtcgg gtacggcctc gagggcgcga tcccggacgc gcgcgcgcac cggatcgacg
      241 cggagatcat cgcgccgcgc ttcagggccg gcgacgtcgc cggcgggctc gaggccgggg
      301 ccgacgcgat cttcgccgcg gcacggggcg agccgtacga agggacgggg aaaacggcgg
      361 ccgagacgga cgggggacgc cggacccgta cgttcgtccg ggtcgccctg ctgtccgcgt
      421 tcgccctcgg gttcggcttc ctgatcgtcg taacgtggct ccgcttccgg cgccggaggg
      481 cacctgggtc tcgtctcgcg acgtcttcct ccgagccgtc gtcctcgtcc ggctggtctt
      541 cggattcgtc ctcctcgtcc tcctcgtcct cctccgactc gtcttcgtcg tccgacttct
      601 cgggcggcgg aggcgacagc gggggcggcg gctcgagcga ttcggactga caccgatgcg
      661 cctcgcggcc gtcctcgcgc tctccctttc cgccgcgccc gcggccgtgt ccgccgcagg
      721 gccctccgtc ccgcccgcgc cgacgcgctt cgtgaccgac aaggccggcg cgctctcccc
      781 ggacaccgcc gcccgcctcg agcagcgcct cgagagcttc gagaaggaga cctcgaacca
      841 gttcctcgtc tacaccgagc gcgtcgtgcc cgacgggacg acgctcgagg agtacaccgt
      901 cgcgtgcgcc caggcctgga aggcgggcca gactcagcgc aagaacggga tgatcctgtt
      961 cgtcttcccg gggtcgcgga aggtgcgcct cgaggtcggc tacgggctcg agggcgccat
     1021 gcccgatgcg cttgcccgcc gggtcctgga cgagcaggtg ctcccgcggt tccgcacggg
     1081 cgactacgac ggcggcgtct cggccggcgt cgaggccgcg atcgccgcga cgaagggcga
     1141 atacaagggc tcggggacga cgcaggcgaa gaagaaggga cgcggcgaga tcccgatccc
     1201 cttccccgtg ttcctcctcc tgctcttcct cttcttcttc gtcatcctcc ccgcggcgcg
     1261 cggggggcgc cgcggcaagt actggtactt gagcagcggc ggctttggcg gaggcggctt
     1321 cggtggaggc ggcttcggcg gcggcggcgg tggcggcttc tcgggcggcg gcggctcttt
     1381 cgggggcggc ggcgcgagcg gagactggtg atgggcgaaa aacccgcgaa ggcgttcttc
     1441 gagagcctcg accatccggc gatcgtcgag gccattcggc gagccgaggc gaggagcttc
     1501 ggcgagatcc gcgtccatct ccaccacgga tccgtggccg acgcgcgggc cgaagccgag
     1561 aagacgttcc tgaggctcgg gatggacaag acggcccggg ggtcgggttg cctgatcttc
     1621 ctcgcgcccg aatcgcgggc cttcgcggtc atcggcggtg cggggatcca cgaaaaagtc
     1681 ggcgacggct tctggctcga ggcccgcgac gccgcgcgcg agcggttcac cgaggggaag
     1741 ttcaccgaag gaatcgtcgc cgccgtcgag aagctgggcg aggccctcgc gctcttcttc
     1801 ccgagagacg gcacgtccga ccgcaacgag cttcccgacg acgtcagcga ggactgaaga
     1861 ccatgaagcg agtcacgctc cccttttgcg ccctccttct gctcgccggg acggcgcgcg
     1921 cccagaccgc cccaccgcat cccaccgcgg acgacgccgt ccggttcgtc gaggcggcag
     1981 aaaagcgcct gctggctctc accgtggagg cgagccgcgc gggctgggtg caggcgaact
     2041 tcatcacgca ggacacggag gccctctcgg ctctcgcgtc cgagcgcctc atcagcgcca
     2101 cggcggagct cgcgaagcag gccgagacgt tccgcggcgt cccggtgccg cctgagacgg
     2161 cgcgcaagct ggagaagatc cgcctgtcgc tggacctcgc ggcgcccgcc gaccccgcga
     2221 agagcgcgga gctcacgcgc ctcgcgaccg gcctggacgc cgcttacggc cgggggcgtg
     2281 catgcctgcc gggcgccaag gagtgcctca cgttcgaaga cgtgaaccgc cggatggcgg
     2341 cgagccgcga cccgcaggaa cttctcgcgc tctggaaggc ctggcatgcg gtcgggacac
     2401 cgctgcgcgc cgattacgcc cgcttcgtca cgctcgccaa cgagggcgcg aaggagctgg
     2461 gcttcgcgga caccggcgcg atgtggcgga gcaagtacga catgccgccc gacgccttcg
     2521 cgaaggagct ggaccggctc tgggatgagg tgaagcccct ctacgtcgcc ctccatacgt
     2581 acgtgcggac gaagctccgg gaaacctacg gcgacgccgt cccgtcgaag ggtccgatcc
     2641 cggcccacct cctcgggaac atgtgggcgc aggactggac gaacgtgtac ccgctccttg
     2701 cgccgaagag cgcggacccc ggctacgacc tcacgaagat cctcgagggg cggaaggtcg
     2761 acgcgaaggg gatggtgaag tacggcgagg gtttcttcac gtcgctcggc ttcgccccgc
     2821 tgcccgccac gttctgggag cgttcgctct tcacgcgccc gcgggaccgc gacgtcgtct
     2881 gccacgcgag cgcgtgggac gtcgacagcg agagcgacat ccggatcaag atgtgcacgg
     2941 agccgaccgc cgaggacttc gtcacggtcc accacgagct cggccacaac ttctaccagc
     3001 gcgcctacgc gaagcagccg ccgctcttcc gcgagagtgc gaacgacggc ttccacgagg
     3061 cgatcggcga cgccgtggcg ctttcgatta ccccgaagta cctcgtcagg ctcggcctcg
     3121 tcgagaaaga gccgccgccc gccggcgaca tcggcttcct gctcaaccgg gcgctcgaaa
     3181 aggtcgcatt ccttccgttc ggcctcctga tcgaccagtg gcgctggaga gtcttcgcgg
     3241 gcgaggtgac cccggccaac tacaacgccg cgtggtggga gctgaagcgg aagatccagg
     3301 gcgtcgtgcc gcccgaggtc cgctccgagg ccgacttcga cccgggcgcg aagtaccacg
     3361 ttcccgggaa cgtcccctac atgcgttact tcctggctca cgtcctccag ttccagttcc
     3421 acagggcgct cgcgcaggcc gcggggtgcg cggaaccgct ccaccgctgc tcgatctacg
     3481 gcagcaagga agccggccgg cgcctcaacg agatgctcga gatggggtcc tcccgcccct
     3541 ggccggaggc gctcgagaag ctcacgggcc agcggaagat cgacgccacg gccctcctcg
     3601 actacttcgc ccccctccgg aagtggctgg acgagcagaa cgccggaaag ccttccggct
     3661 ggtgaggttg acgagcggtc cttcgtcctt ttgacagggt gcgggccctc tggtattttt
     3721 gccggccctt ttgacctctc cgaggtcggg gtttcaggag aagccatgta cgcagtgatc
     3781 cgcaccgggg gcaagcaggt ccgcgtcgcc gaaggcgacg tggtccgcgt cgagcgcctc
     3841 acgaaggaga ccctgaaaaa gggcgccgag gtgtccctcg gagaagttct cgcgatcggc
     3901 agggaggacg gcctgcacct cggctcgccc ctcgtcgagg gagcgagcgt gacggccgtg
     3961 gtcgtccggg agacccgggg tcccaagctg ctcgtttaca agaagaagcg ccgcaagggc
     4021 ttccagcgca cccatggtca ccggcaggac ctcgtcgagg tccgcatcgc ctcgatcaag
     4081 gcctagggcg gagacggaca aatggcacac aaaaaaggtc agggctcgtc ccgcaacggc
     4141 cgcgacagca acgcgcagcg cctcggcgtg aagcgcttct caggccagct cgtcaccggg
     4201 ggctcgattc tcgtccggca gcgcggcacg ccgctcaagc cgggcctgaa cgtcggccgc
     4261 gccaaggacg actcgctctt cgcgaaggtg gacggcgtcg tgaacttcca cgacaagggc
     4321 cgcctcgggc gtttcgtctc gatcacgccg gtcgaacaaa agcccaacta acccatgttc
     4381 atcgacgaag ccgccatcca cgtcgccggg ggcgacgggg ggaacggctg cgtcgcgttc
     4441 cgccgagaga agttcatccc gcacggcggt ccctcgggcg gtgacggcgg tgacggcggg
     4501 tccgtcacgc tcgtcgcgga cccctccctg aacacgctct acccgttccg cttcacgaag
     4561 cgcttcgtcg cgggccgcgg cgggcacggc aggggaaaca actgccacgg gaagaacgga
     4621 cgcccgctgg aagtctcggt gccggtgggc accgtcgtga aggaccgcga gtcgggcgag
     4681 atcctgggcg acctcgtcgc gcaggggcag acgctcctga tcgctccggg cggccgcggg
     4741 ggccgcggca acgcccgctt cgcgacctcc acgaaccgta cgccgcgccg cgccgatccg
     4801 ggcacggagg gcgtcacgcg cgagctggcg ctggagctca agctcctggc ggacgtcgcc
     4861 ctcgtcggcc ttccgaacgc cgggaagtcg acgctcatcg cggccatctc ggcggcgcgg
     4921 ccgaagatcg cggactaccc gttcacgacg ctcgtcccga acctcggcgt cgtgtcgtgg
     4981 gaccggttcc ggacgttcgt cgccgccgac gtgccgggcc tcatcgcggg cgcgcacgag
     5041 ggtcacgggc tcgggatccg gttcctcaag cacgtcgagc gctcacgcat cctcgcccac
     5101 ctcgtcgacg cggcgtccct ctcggcggag gatcccgccg gcgacgcgga ggcggcggtc
     5161 gcgacgatcg agaacgagct gacggcgttc cagcccgagc tcgcgaggcg cgagcgtgtg
     5221 ctcgtcgcga cgaaagcgga cgccgcgacg cccgaacaga tcgaggccgt ctctcgagcg
     5281 gccaggcggc gcggcctgac gttcttcgcg atctcggccg cggcccggca gggcctcgag
     5341 ccgctcgtcg cccacctggg gacgcgcctc gacgccctgg ccgctctttc gacgcccgtt
     5401 ccggtccccg ggagcccgga ggccccgtga cgctccggat cggggtctac ggcggcacgt
     5461 tcgaccccgt ccacgtcggg cacgtcttcc ccgtcgagga gacgcgcgtc aacctcggcc
     5521 tcgaccgcgt cctgtacgtc cccgcctccc agccgccgca caagccggcc tcgccgtcgg
     5581 cgccggcgca tcaccgcttc gcgatgctcg cgctcgcgct cgagccgtat ccgaacctcc
     5641 tcctctcgga tttcgaggtc gcgcggggcg gaacgaccta cacgatcgag acgcttcgcc
     5701 atctgcgcgc gcagcgggag gacgccgaga tcctgctcgt cctcgggtcc gattcgctcg
     5761 ctcagatcga gacgtggcgg tctttccgcg agatcctcgc ggaattccgg ctggcggtgg
     5821 tcgcgcgcga gggattcaca cgcgaggcgc tgttcggggc gctgccgaag gacgtcgccg
     5881 ggcgctttgc cccggaaggc tcccggcccg gcggggacgt cccggagcag acgatctact
     5941 ggggaggcaa cgctccggtt acaatttcgt ccacgtggct aaggcgcgcg atccccgcgg
     6001 gagaagacct gagcggaagc ctcccggaga acgtggccgc gtacctcaga aagcacgccc
     6061 tctaccgcct cccgtgaagc atcccgtccc ccccatcggg gacgtggacg acctcatcgc
     6121 ggcgacgctc gcgcgcgcaa ccggcccggc cggcgaacgg ccccagcggc ccgcggacgc
     6181 cgagctcgac gcgcggctca cggcgcacct caagaggggc gtggcggcgc tcctcgacaa
     6241 aaaagcggag aaactcgtcg tcctgaacct ccagggcctc acggcgatgg ccgactactt
     6301 cgtcctcgcg acggcctcga gcgaccggca ggcgcaggcg ctcgcggacg ccgtcgaggc
     6361 ggccctcaag gccgaggggc gccggcccct ctcgatcgag ggctactcct cgccgtggat
     6421 cctcgtggac tacggagacg tcgtgttcca catcttccac gacgaggcgc gccgtttcta
     6481 cgggctcgag cgcctctggg gcgacgcgcc ggacgcgacc gcgtcgttcc gctgacgcgc
     6541 gtgcccgccc gtacgcgcgc ggccgcgccg gcgccgctgt tcttcgcgac gcggcaccgc
     6601 ggcctcaggg agcagctccg ccgcctcgcc cgcgccgccg actccgcgaa cacggtcctc
     6661 ctcctcgggg cgccgggcac gggcaaggac cgcctcgcgc gcctcctgca cgagcgctca
     6721 cggcgtgccg ccgagccgtt cgtgcgcgtg gacctcgcgg cgatcgcgga cgatctcttc
     6781 gagagcgagc tcttcggcca cgagcgcggc gccttcacgg gagcggtgtc cccgaagccg
     6841 ggcctcctcg agggcgcggg cgccgggacc ctttatctcg accggatcga cgcgctgtcc
     6901 ctgcgcgcac agggaaagct gctccgcgtc gtggaggagc ggctcgtccg ccgcgtgggc
     6961 ggaacgcggc agacggcgct ccgggcgcgc ctcgtcgcgt cggcgggccc cgacctgccg
     7021 cgccgggcgc ggagcggcga attccgcgag gatctcttct accg
//
LOCUS       DAOMFY010000207         7022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3472_length_7022_cov_7.830487, whole genome shotgun sequence.
ACCESSION   DAOMFY010000207 DAOMFY010000000
VERSION     DAOMFY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3472_length_7022_cov_7.830487"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLB01_14485"
     CDS             complement(<1..382)
                     /locus_tag="PLB01_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78559.1"
                     /translation="MPGRGDPLIQTVAAAPAVTRLAGRIEEGVLGTSTGVVSLTGAPP
                     GLFPYLLEAVGGPLGLKWAVVFAHEKDAAAFARDAASVLGAPRVALFPAPALSPYQGI
                     APSLKVRRDEFGALQRLASGEVTVL"
     gene            496..1512
                     /locus_tag="PLB01_14490"
     CDS             496..1512
                     /locus_tag="PLB01_14490"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ78560.1"
                     /translation="MFAFLLAAPLAADQKQKEEVEGCLSCHGDKSASFKLPDGSTQSV
                     FVDAGQFAKSVHGTKIACTGCHPAQAELPHPEVKAKTKAEFAASFKDLCKSCHFENYT
                     KALDGVHFAVIQKGGAAPFCTDCHGSHDVSKPSVPRTRVSDTCGGCHADIADVFAKSV
                     HGAALAQGNQDVPVCVDCHKAHDISDPRTTEWKLKLVDACAKCHTNKAVMAKYKLSTN
                     VVQTYLNDFHGMSASLASSGQTPRVITALCVDCHGTHNITKAKDGNSQVLQANLQKTC
                     AKCHPGAPPNFTAAWLSHYEPSPKRAPLVWAVGAFYKIFIPFLIGGLVLQILLHLWRV
                     MVNR"
     gene            1509..>2170
                     /locus_tag="PLB01_14495"
     CDS             1509..>2170
                     /locus_tag="PLB01_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQQ78561.1"
                     /translation="MTEYAVRFSKKQRVEHFVVMTTFVLLALTGFPQKFYEAGASHFL
                     VKIFGGLDGARLVHRVSGLLFAALTFVHLSLAILSTLQHKMKMTLVPDRQDFKDAVQQ
                     LRYYLGLTNEEAKFDRFDYRQKFEYWGLVLGGLVMIATGFILYFPMLVTRFLPGELIP
                     MAKVAHSNEGLMAFLVVITWHIYNAHLAPGIFPYDPAIFTGKIPLERLKHEHRREYDR
                     LYG"
     assembly_gap    2171..2270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2299..3771
                     /locus_tag="PLB01_14500"
     CDS             2299..3771
                     /locus_tag="PLB01_14500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ78562.1"
                     /translation="MPRLSSGPPARIRDRHGLQRRAVLLVLFGLLVPVAILAELGRRA
                     LDDVSHRLARERELLARSVAVAVELTVSDPNYPDRSLQEARAGLLLRAFREDRTLAVE
                     LLDETGCAFASSYALPESPDDVVASAPIASTAWRVRIRQPHRVAFAEETVLERWLLFL
                     TPVFLVLAGIFAWGAARSVRRPLRTLTESADRLAAGDLSLPVPLPEEKSRDDEVGRLA
                     RVFESMRVALAASMAEVTQANATLERRVAARTAELQKLYAELKDRDARRAELVRKLLS
                     AQEDERRRIARELHDETCQTVAALAVGLDTVRRAATPEEASTKLEDARALVSRTLDGL
                     HRVIFDLRPSVLDDLGLVSAVRWWVSRHLTPAGITARLEVEDLDERLPSTVEIPVFRA
                     VQEALTNALRHSGAKTVLIQMSREGGTLSVDVEDDGRGFNPADVATPSETGQGLGLLG
                     MRERIEILGGTLTIDSSPGAGTHVAFTIPVPREEPARAAH"
     gene            3758..4408
                     /locus_tag="PLB01_14505"
     CDS             3758..4408
                     /locus_tag="PLB01_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ78563.1"
                     /translation="MPRIRVVIADDHALLREGLRALLKTAEDIEVVGEAADGLEAIEA
                     CRTLGPDVVLMDIAMPGLGGLEAALEVRKVSPHTRVLVLTQYDDREYVSRFLKAGAAG
                     YILKKAAGTELVSAIRGVSRGGLVLDPAIARDAMGSDAPAGSGAGEDPYDLLTDREKQ
                     VLKLVAEGKANKEVAALLDIAVKTAMSHREHVMEKLGLHGRTELIRFALRRGIIQG"
     gene            4563..5522
                     /locus_tag="PLB01_14510"
     CDS             4563..5522
                     /locus_tag="PLB01_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78564.1"
                     /translation="MKKLLRFLAFASLSAFAFSAAPGFAADSVTILSMTPASPAGLAG
                     VPPATPMGPGNSIEVVFAYNLETQATGRIGFYTAGEPGNPPHTGVEIPFMVAKKGKGE
                     GKTRFSVQCPQGFAGCTIKSVRFNLFFDGPGGLMKLFEGHKAVDYTFKCATVGVPPGG
                     GPSDRKPNITSARPGLYIWGSDPAKKQWVDWNGSVKLKAADSINPHPTPSNGQCAFNV
                     EYYEKETNGVATGNFKNKLYSDANERAINGPFALAASEAKSLVTQPYLDSGAHGLKLV
                     LDAEGNVAETNEGDNSFSIRYHLEGTCDSRPATAPAGAAAPRK"
     gene            complement(5563..6900)
                     /locus_tag="PLB01_14515"
     CDS             complement(5563..6900)
                     /locus_tag="PLB01_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78565.1"
                     /translation="MRLRNLSLACVLAAGAAASAAAQDSMLAGSNPFVSDSGKLRLKV
                     EIKAAFRDSRDLSLTVANTGLPFPVVMQTVDPGAHVEVPNLGLTLEADLTPDVFARAV
                     VNVLDLYNRNPTSSADKILLREAFVRFGKKVETLRVADGTSVYLELGKFPRFSKQVVR
                     RLESYGLWGTAVGRFEETGAEAGGSFGPHVYWRASVTGGTPLFFRDPNALAGDNGTPE
                     RTVGSTTPVVYNSGFPILYDTVAPDVGGSGKMQLGGGAGLRWNWGEGGRSGVDVLAWI
                     FRRDLADRVSLHGTFYGADLRLLQGVGVSLPIEGNEKIEWGVNVEARLGPVHFFGQAI
                     KQTLAYLWRQGWEAELSWRIPLPGLFASGDQPVVNWIEPEVRISYVDNLWENPAGFVA
                     PSTGWDWRKYDYGVRIGIVRGLDLTAEYARHDATTRRGFVHPDEWLVTLRAAF"
BASE COUNT         1079 a         2533 c         2303 g         1007 t
ORIGIN      
        1 cgaggaccgt gacctcgccc gaggcgaggc gctggagcgc cccgaactcg tcgcgacgga
       61 ccttgagcga aggggcgatt ccctggtacg gcgagagcgc cggagccgga aacaacgcga
      121 cgcgcggggc gccgaggacg gaggccgcgt cgcgggcgaa cgcggccgca tccttctcgt
      181 gggcgaacac gacggcccac ttcaggccga gagggccccc gacggcctcg aggaggtacg
      241 ggaagagccc cgggggggcc cccgtcagcg acaccactcc cgtcgacgtt ccgaggaccc
      301 cctcctcgat ccgcccggcg agacgcgtca ccgcgggtgc ggcggcgacc gtctggatga
      361 gcggatctcc ccgccccggc acgggccgag aatatccgta attccgttgc ttcattgacc
      421 cccccgggcc ccgcgggcac cgtgcccgcg gaggctcgtg tgatcaccgg ccgcaaaggt
      481 ctcatttccg gcgccttgtt cgctttcctc ctcgccgcgc cgctcgccgc cgaccagaag
      541 cagaaggaag aggtcgaggg ctgcctctcc tgccacggcg acaagtccgc ctcgttcaag
      601 ctgcccgacg gctccacgca gtccgtgttc gtcgacgccg ggcaattcgc gaagtccgtc
      661 cacgggacga agatcgcctg cacgggctgc caccccgcgc aggccgagct cccgcacccc
      721 gaggtgaagg cgaagacgaa ggccgagttc gcggcctcct tcaaggacct ctgcaagtcc
      781 tgccacttcg agaactacac gaaggcgctc gacggcgtgc acttcgcggt catccagaag
      841 ggcggcgccg ccccgttctg cacggactgt cacgggtccc acgacgtctc caagccgagc
      901 gtgccccgga caagggtctc ggacacgtgc ggcggctgcc acgcggacat cgcggacgtc
      961 ttcgcgaaga gcgtgcacgg cgcggcgctc gcgcagggga accaggacgt tcccgtctgc
     1021 gtggactgcc acaaggccca cgacatctcg gacccgcgga cgacagagtg gaagctcaag
     1081 ctcgtcgacg cgtgcgcgaa gtgccacacg aacaaggccg tcatggccaa gtacaagctc
     1141 tcgacgaacg tcgtccagac ctacctgaac gacttccacg ggatgagcgc gtcgctcgcg
     1201 agctcggggc agacgccgcg cgtcatcacg gcgctctgcg tcgactgcca cgggacgcac
     1261 aacatcacga aggcgaagga cggcaactcc caggttctcc aggcgaacct ccagaagacc
     1321 tgcgcgaaat gccatccggg ggcgccgccg aacttcacgg ccgcctggct ctcccactac
     1381 gagccgagcc cgaagcgcgc cccgctcgtc tgggccgtcg gcgccttcta caagatcttc
     1441 atccccttcc tgatcggcgg gctcgtcctg cagatcctcc tgcacctctg gcgcgtgatg
     1501 gtgaaccgat gaccgaatac gccgtccgat tctcgaagaa gcagcgcgtc gagcacttcg
     1561 tcgtcatgac gacgttcgtg ctcctcgccc tcacggggtt cccccagaag ttctacgagg
     1621 ccggtgcgtc gcatttcctc gtgaagatct tcggaggact cgacggcgcg cgtctcgtcc
     1681 accgggtctc gggcctgctc ttcgcggcct tgacgttcgt ccacctgtcg ctcgcgatcc
     1741 tctcgaccct ccagcacaag atgaagatga ccctggtgcc ggaccggcag gacttcaagg
     1801 acgccgtcca gcagctccgc tactacctcg gactcacgaa cgaggaagcg aagttcgacc
     1861 ggttcgacta ccggcagaag ttcgagtact ggggcctcgt cctcggcggc ctcgtgatga
     1921 tcgcgacggg cttcatcctc tacttcccga tgctcgtcac ccgcttcctt cccggcgagc
     1981 tgatcccgat ggcgaaggtc gcgcacagca acgaggggct catggctttc ctcgtcgtga
     2041 tcacgtggca catctacaac gcgcacctgg ccccgggcat ctttccctac gacccggcga
     2101 tcttcaccgg aaaaatcccc ctcgagcgcc tcaagcacga gcaccggcgc gagtacgacc
     2161 gcctctacgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccgcgccgg
     2281 agaagcccgc tccggcggat gcctaggctc tcgagcggcc cgcccgcgcg catccgggac
     2341 cgccacggcc tccagcggcg ggcggtcctt ctcgttctct tcggcctcct cgtcccggtc
     2401 gcgatcctcg ccgagctcgg ccggcgcgcg ctcgacgacg tgtctcaccg cctcgcccgc
     2461 gagcgcgagc tcctcgcgcg gtcggtcgcc gtggccgtgg agctcacggt ctcggatccg
     2521 aactatcccg accgttcgct ccaggaggcg cgcgccggcc tcctcctgcg cgcgttccgc
     2581 gaggaccgaa ccctcgccgt ggagctgctc gacgagaccg gctgcgcctt cgcttcgagc
     2641 tacgcgctgc cggagtctcc cgacgacgtc gtcgcgtcgg cgcccatcgc ctcgacggcg
     2701 tggcgcgtgc ggatccgcca gccgcaccgc gtcgccttcg ccgaggagac cgtcctcgag
     2761 cgctggctcc tcttcctgac gcccgtcttc ctcgtcctcg cggggatctt cgcctggggg
     2821 gcggcgcgca gcgtgcggcg ccccctccgg acgctcacgg aatcagccga ccgcctcgcg
     2881 gcgggcgacc tctccctccc ggtcccgctg ccggaggaaa aatcgcgcga cgacgaggtc
     2941 ggccgcctcg cacgggtctt cgagtcgatg cgtgtcgcgc tcgcggcctc catggccgag
     3001 gtcacgcagg cgaacgcgac gctcgagcgc cgcgtcgcgg cgcgcaccgc cgagctccag
     3061 aagttgtacg ccgagctcaa ggaccgcgac gcccgccggg cggaactcgt ccggaagctc
     3121 ctctcggccc aggaggacga gcggaggcgg atcgcgcgcg agctccacga cgagacgtgc
     3181 cagaccgtcg cggcactcgc cgtgggcctc gacaccgtcc gccgcgccgc cactcccgag
     3241 gaggcgagca cgaaactcga ggacgcgcgc gccctcgttt cgcgcacgct cgacggcctc
     3301 caccgggtga tcttcgacct tcgcccgtcc gtcctcgacg acctcggcct cgtctcggcc
     3361 gtacgctggt gggtatcccg ccacctgacg ccggccggca tcacggcgcg gctcgaggtc
     3421 gaggacctcg acgagcgtct cccgtcgacc gtcgagatcc ccgtttttcg tgcggtccag
     3481 gaggcgctca cgaacgccct ccggcactcc ggtgcgaaga ccgtgctcat ccagatgtcg
     3541 cgcgagggcg ggacgctttc cgtggacgtg gaggacgacg gccgcgggtt caaccccgcc
     3601 gacgtcgcga cgccctcgga aaccgggcag ggcctcggcc tcctcggcat gcgcgagcgc
     3661 atcgagatcc tcgggggtac tctcacgatc gattcgtcgc cgggagcggg aacgcacgtc
     3721 gcgttcacca tcccggtccc gagggaggag cccgcccgtg ccgcgcatta gggtcgtgat
     3781 cgcggacgac cacgcgctgc tgcgcgaggg gctccgcgcg cttctgaaga ccgcggagga
     3841 catcgaggtc gtcggtgaag ccgccgacgg gctcgaggcg atcgaggcct gccgcacgct
     3901 cgggccggac gtcgtcctga tggacatcgc gatgccgggc ctcggggggc tcgaggcggc
     3961 tctcgaggtc cgcaaggtga gcccgcacac gcgcgtcctc gtcctgacgc agtacgacga
     4021 ccgcgagtac gtgtcacgct tcctcaaggc cggcgccgcg gggtacatcc tcaagaaggc
     4081 cgcgggcacc gagctcgtgt cggcgatccg cggcgtctcc cgcggcggtc tcgtcctcga
     4141 ccccgcgatc gcgcgtgacg cgatggggag cgacgcgccc gccggctccg gcgccggcga
     4201 ggacccgtac gacctcctca cggaccgcga gaagcaggtc ctcaagctcg tcgccgaggg
     4261 caaggcgaac aaggaagtgg cggcgctcct cgacatcgcc gtgaagaccg cgatgtcgca
     4321 ccgcgagcac gtcatggaga agctcggcct ccacgggcgc accgagctga tccgcttcgc
     4381 gctccgccgc gggatcatcc agggctgaag gtcgagactc cttcgagggt gattcgagtc
     4441 acccttcggg gcggcccggg gaagtctggc gctcgcggac cgttcttctt cgcgcccaga
     4501 ccatccgtgg gcctcgacag gggccgcgga cggccgcacg ttgtcaggca gaaaggaagg
     4561 ccatgaagaa actcctccgt ttcctcgcgt tcgcgtccct ctctgcgttc gccttctcgg
     4621 cggcacccgg gttcgccgcc gactcggtca cgatcctgag catgaccccg gcgtccccgg
     4681 cgggcctcgc cggggttccc cccgcgaccc cgatgggtcc cggcaactcg atcgaggtcg
     4741 tgttcgcgta caacctcgag acgcaggcca ccggccgtat cgggttctac acggccggcg
     4801 agcccggcaa ccccccgcac acgggcgtcg agattccgtt catggtcgcg aagaaaggca
     4861 agggcgaggg gaagacccgc ttctccgtcc agtgccccca gggcttcgcg ggctgcacga
     4921 tcaagagcgt gcgcttcaac ctgttcttcg acggtccggg cggcctcatg aagctcttcg
     4981 agggccacaa ggccgtcgac tacacgttca agtgcgccac cgtcggcgtc ccgcccggcg
     5041 gcgggccgag cgaccggaag ccgaacatca cgtcggctcg tccgggcctc tacatctggg
     5101 gcagcgaccc cgcgaagaag caatgggtcg actggaacgg ctcggtcaag ttgaaggccg
     5161 ccgattcgat caacccgcac ccgacgccga gcaacggaca gtgcgccttc aacgtcgagt
     5221 actacgagaa ggagacgaac ggcgtcgcga cgggcaactt caagaacaag ctctactccg
     5281 acgcgaacga gcgcgcgatc aacgggccgt tcgcgctcgc cgcgagcgaa gccaagagcc
     5341 tcgtcaccca gccgtacctc gactccggcg cccacggcct caagctcgtc ctcgacgccg
     5401 aagggaacgt ggcggagacg aacgagggcg acaacagctt ctcgatccgg taccacctcg
     5461 aggggacctg cgacagcagg cccgcgacgg cgcccgccgg cgcggctgcc ccgaggaagt
     5521 gatccgcggc cgcgggaagg ctccctcctc cccgcggcgc tttcagaacg cggcgcgcag
     5581 cgtcacgagc cactcgtccg ggtggacgaa tccgcggcgg gtcgtggcgt cgtgccgcgc
     5641 gtattcggcc gtgaggtcga gcccgcgcac gattccgatg cgcacgccgt agtcgtactt
     5701 ccgccagtcc cagcccgtgg acggcgcgac gaagcccgcc gggttctccc acaggttgtc
     5761 gacgtacgag atccggacct cgggctcgat ccagttcacg accggctggt cgccggaggc
     5821 gaagaggccc ggcagcggga tgcgccacga gagctcggcc tcccagccct gccgccacag
     5881 gtacgcgagg gtctgcttga tcgcctgccc gaagaagtgg accgggccga ggcgggcttc
     5941 gacgttcacg ccccactcga tcttctcgtt gccctcgatc gggagcgaga cgccgacacc
     6001 ctggaggagg cggaggtccg ccccgtagaa cgtgccgtgg aggctgacgc ggtccgcgag
     6061 gtcgcgccgg aagatccagg cgaggacgtc gacgccgctt ctcccgccct ctccccagtt
     6121 ccagcgcagg cccgcgccgc cgccgagctg catcttcccg gagcctccga cgtcgggcgc
     6181 gaccgtgtcg tagaggatcg ggaacccgga gttgtacacg accggcgtcg tggagccgac
     6241 cgtccgctcc ggcgtcccgt tgtcgcccgc gagcgcgttc gggtcgcgga agaagagcgg
     6301 cgtcccgccc gtgacgctcg cgcgccagta gacgtgcggc ccgaaggagc cgcccgcctc
     6361 ggcgccggtc tcctcgaagc gcccgacggc ggtcccccag aggccgtagc tctcgaggcg
     6421 gcggacgacc tgcttcgaga agcgcggaaa tttcccgagc tcgaggtaga cgctcgtgcc
     6481 gtcggcgacg cgcagcgtct cgaccttctt cccgaagcgc acgaacgcct cgcgcagcag
     6541 gatcttgtcg gcgctcgagg tcgggttgcg gttgtagagg tcgagcacgt tgacgaccgc
     6601 gcgcgcgaag acgtccggcg tgaggtcggc ctcgagcgtg aggccgaggt tcgggacctc
     6661 gacgtgcgcg ccggggtcga cggtctgcat gacgaccggg aacggcagtc ccgtgttcgc
     6721 gaccgtgagc gagaggtcgc gcgagtcgcg gaacgcggcc ttgatctcga ccttgagccg
     6781 cagctttccg gagtcggaaa cgaacggatt cgagccggcg agcatcgagt cctgcgccgc
     6841 ggccgaagcc gcggcgcccg ccgcgaggac gcaggcgaga gataggttcc ggagtctcat
     6901 gccgccccgc cattctacgg ggcggcccgg gcggcacgac ccgctgtggg agcttcaacg
     6961 gcgtgatccc tcgctcggcg atcgtgaacg tcgtcccgcc cctgccgtcg agcgcgagga
     7021 cg
//
LOCUS       DAOMFY010000208         7014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3481_length_7014_cov_9.358385, whole genome shotgun sequence.
ACCESSION   DAOMFY010000208 DAOMFY010000000
VERSION     DAOMFY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_7014_cov_9.358385"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLB01_14520"
     CDS             complement(<1..771)
                     /locus_tag="PLB01_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HQQ78566.1"
                     /translation="MKRVLVGVLATILLFLPFAASASSVERDAPNAEPFVFQGQSWVS
                     QKAFIESGARCSTRPVDDEEADLIELETERLLAERGGRHPMVTGGTIPVYFHVINNGT
                     GIANGDVPDSQITQQLNVLNAAYASTGWQFTLAGTDRTTNASWYTAQPGTTAETQMKN
                     ALRQGSADDLNIYSNNMGGGLLGWATFPADYARKPKMDGVVILYSSLPGGTAAPYNLG
                     DTATHEVGHWMGLYHTFQGGCSGSGDSVSDTPAERSAAF"
     gene            883..1026
                     /locus_tag="PLB01_14525"
     CDS             883..1026
                     /locus_tag="PLB01_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78567.1"
                     /translation="MLPGDAKADYLRVPVIGFALLATAAFNKAFRHAGTPAQKEAHVR
                     HGD"
     gene            complement(1041..1439)
                     /locus_tag="PLB01_14530"
     CDS             complement(1041..1439)
                     /locus_tag="PLB01_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HQQ78568.1"
                     /translation="MALVRASFESLRPIPRGVGRAFYEKLFDLDPSLRALFKGDLDAQ
                     GAMFVSALGLAVAGLEDAHSGERPLRDLGQRHAAYGVTDTHFATFREALVRTLRDQIG
                     AGFTEAHAAAWRAAFDRIGVVMRDAASGRS"
     gene            1578..2567
                     /locus_tag="PLB01_14535"
     CDS             1578..2567
                     /locus_tag="PLB01_14535"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ78569.1"
                     /translation="MKATPYPSIVTRSERMNVLLERLPTVAASDGSVLLVGETGVGKE
                     LFADTVHRLSPRRDGPLVKISLSAVPHELLESELFGHERGSFTHAIQTKKGLFEMAHG
                     GTLFLDDVDDVPPSIQPKLLRVLESGEVMRVGATTTIPVDVRLVSASKVNLRELVDRG
                     LFRADLYYRLNVVPVEIPPLRLRGEDVRLLAEHFLQRFAPGRGLRLSEAAARSLERYH
                     WPGNVRELRNVIQRVSLFAREEVGLEDLPPEILDGNPVELLARACARCFSEQSLSFGQ
                     VVECLETNLLRQALKDAGGNRSQAARALGLSLSTMRDKLKKYGLDGGADLDID"
     gene            2798..2995
                     /locus_tag="PLB01_14540"
     CDS             2798..2995
                     /locus_tag="PLB01_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78570.1"
                     /translation="MIEVSLRQGRRYLTVALEPHCLHASTGIPEDTHLGDRVRVEAEI
                     RVVNVAPELPHDIRQPARRPA"
     gene            3013..4137
                     /locus_tag="PLB01_14545"
     CDS             3013..4137
                     /locus_tag="PLB01_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HQQ78571.1"
                     /translation="MSEPRPTIWEQARMSGYSRRDFVQFCAWMAAAAGIHESALGAVV
                     QAMDTKPRLPVVWFHFQECTCCSESFIRSSHPIVGDIVLDKISLDYTLTLQAAAGHQA
                     EAALNETMKKYKGQYLMLVEGSVPTAEGGVYCCIAGRTALDIVKEAAANAKAIVAWGS
                     CASNGCIQAANPNPTSATPVHKLISGKPIINVPGCPPIAEVMAGTVVHLLAFDRIPQL
                     DGLGRPMAFYSRRVHDTCYRRPNYDAGLFVETFDDENARRGYCLYKVGCRGPVTYNSC
                     GVIRWNNGVSYPIQSGHGCIGCSEANFWDNGPFYRHLPSFPGFGIETTADKVGVAAGA
                     AVAVGIAAHAVTTNIAKRKQIREEIHKSDRTPEEVTGPHK"
     gene            4145..5863
                     /locus_tag="PLB01_14550"
     CDS             4145..5863
                     /locus_tag="PLB01_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQQ78572.1"
                     /translation="MSNRIVVDPITRIEGHLRIEAEIKDGKIVDAFSSGTMVRGFELI
                     LKGRDPRDAWAFTERACGVCTTVHALASVRAVEDALKIVVPPNAELVRNLMFCAQYLQ
                     DHVVHFYHLHALDWVDVVSALKADPKKTSEIAQSISNWPKSSPGYFSDLQKRLTAFVG
                     SGQLGIFANGYWGHSAYKLPAEVNLLGVAHYLEALEWQKEIVKVHTIFGGKNPHPNYL
                     VGGAPCSFNIEEASAINAERLAYVGTLFDQATEFVEKVYLPDLMAVASFYKDWGAIGG
                     GLPNYMAYGDLPTNGYADVAKFKFPRGAILNRNLAEVLPIDGKDEKQIEEYISHSWYE
                     YGAGDGAGLHPWKGETKLRYSGPKPPYEHLEVDKKYSWLKTPRWKGNAMEVGPLARCL
                     VAYASGSAEVKELVDGALKQLNVPVGALFSTLGRTAARGLETKLIARWAKEFYGQLLA
                     NIKNGDSRTFNGEKWEPATWPVEAKGVGLSEAPRGALAHWIVIKDRKIDNYQLVVPST
                     WNASPRDAKGQRSAYEAALVGTPVANPEHPLEILRTIHSFDPCIACAVHLYDEKGNHL
                     HQVKVL"
     gene            5874..6557
                     /gene="cybH"
                     /locus_tag="PLB01_14555"
     CDS             5874..6557
                     /gene="cybH"
                     /locus_tag="PLB01_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HQQ78573.1"
                     /translation="MPKPKTEVRRVYVWQLPVRLYHWVNALCVLLLAATGYLIGSPPA
                     IQSAREASFGYWFGTVRFIHFLSAFVFFFNFVFRIYWGFVGNAWARWPQFLPFRKVQR
                     EEAIEVLKVDILQAKAAPLPSIGHNPIAGFTYFLTFLAFLFQSVTGFGMYAAMSHAKL
                     PHLFAWIVPLMGGDFAVRQWHHMTMWFFILFSMVHVYLVFYHDYVEARGVLSSMAGGW
                     KFIERPAEK"
     gene            6554..>7014
                     /locus_tag="PLB01_14560"
     CDS             6554..>7014
                     /locus_tag="PLB01_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HQQ78574.1"
                     /translation="MSNDRPDEAARPVLVLGIGNVLLGDEGVGVHAVRRMEPETWPPN
                     VKLLDGGTGGFHLLSLFQEYERIVLIDATMDGQPPGTVRLIRPRFASDYPRTLSAHDI
                     GLKDLVESAALLGLEPEVVLVVVSVETLPEGLHAELSPEVAAALPRVAALAR"
BASE COUNT         1119 a         2534 c         2255 g         1106 t
ORIGIN      
        1 gaacgccgcg gagcgctcgg cgggagtgtc ggacacggag tcgccgcttc cgctgcagcc
       61 gccctggaac gtgtggtaca ggcccatcca gtggccgacc tcgtgcgtcg ccgtgtcgcc
      121 gaggttgtag ggggccgcgg tcccgcccgg gagcgacgag tagaggatca cgacaccgtc
      181 cattttcggt ttgcgcgcgt agtccgccgg gaacgtcgcc cagccgagga ggcctccgcc
      241 catgttgttc gagtagatgt tcaggtcgtc cgccgagccc tgccggagcg cgttcttcat
      301 ctgggtctcg gccgtcgtgc ccggctgcgc cgtgtaccac gacgcattcg tcgtccggtc
      361 ggtgcccgcc aaggtgaact gccatcccgt ggacgcgtac gcggcgttca ggacgttcaa
      421 ttgctgggtg atctgggaat cgggcacgtc tccgttcgcg atcccggtcc cgttgttgat
      481 gacgtggaag tagaccggga tggttccgcc cgtgaccatc gggtgccggc cgcccctctc
      541 ggcgaggagc ctctccgtct ccaactcgat gaggtccgct tcctcgtcgt ccaccggccg
      601 cgtgctgcag cgggcgccgc tctcgatgaa cgccttctgg ctcacccagc tctgcccctg
      661 gaacacgaag ggctcggcgt tcggcgcgtc gcgctcgacg ctgctcgccg aggcggcgaa
      721 cggaaggaag aggaggatcg tcgcaaggac gcccacgagg acacgcttca tgaaggacct
      781 cctgagattt gttctcagga tcctatcccc cccggctcgt gccttccagg gaattctccc
      841 gatccctctc cggactcttt cctctccccc cagcagtgcg gcgtgctgcc cggcgacgcg
      901 aaggccgact acctcagggt ccccgtcatc ggcttcgcgc tcctcgcgac ggccgccttc
      961 aacaaggcct tccgccacgc cggaacgccc gcccagaagg aagcccacgt ccgccacggc
     1021 gactgagcgg aatgaacgga tcagcttcgg ccggaggcgg cgtcgcgcat cacgacgccg
     1081 atccggtcga acgcggcgcg ccaggccgcc gcgtgcgcct cggtgaaccc cgcgccgatc
     1141 tggtcgcgca gggtcctcac gagcgcctcg cggaacgtcg caaagtgcgt gtcggtgacg
     1201 ccgtatgccg cgtggcgctg cccgagatcg cgcaggggcc gttcgcccga gtgagcgtcc
     1261 tcgaggcccg ccaccgcgag cccgagagcg ctcacgaaca tcgccccctg cgcgtcgaga
     1321 tcgcccttga agagcgccct cagtgacggg tcgaggtcga acagcttctc gtagaacgcg
     1381 cgccccacgc ccctcggaat cggacgcagg ctttcgaagc tcgctcggac gagggccatc
     1441 tcttcgcgcg tcatcctcgg gatcatgcca cggcgcggcg cgggctttcg acggcgcagg
     1501 cctcacgccg ggacccgtca tccgggctgg accccgttgt aggatgcggg cgccatcccg
     1561 gatggaacga agcctcgatg aaggcaactc cctacccgtc gatcgtgacg cgcagcgaga
     1621 ggatgaacgt ccttctcgag cggcttccga ccgtcgcggc gtcggacggt tccgttctcc
     1681 tcgtgggcga gaccggcgtc ggcaaggagc tgttcgccga caccgtccac cgcctcagcc
     1741 cgaggcgcga cggccccctc gtgaagatct ccctttcggc cgtgccgcac gagctcctcg
     1801 agagcgagct cttcggccac gaacgcggct cgttcacgca cgcgatccag acgaagaagg
     1861 ggctcttcga gatggcgcac ggcgggacgc tcttcctcga cgacgtcgac gacgtgccgc
     1921 cgtcgatcca gccgaagctc ctgcgcgtcc tcgagtccgg agaagtcatg cgcgtcgggg
     1981 cgactacgac gatcccggtg gacgtccggc tcgtgagcgc ctcgaaggtc aacctgcggg
     2041 agctcgtcga ccgcggcctc ttccgggccg acctctacta tcgcctcaac gtcgtccccg
     2101 tcgagatccc gcccctccgg ctgcggggcg aggacgtgag gctcctcgcc gaacacttcc
     2161 tccagcggtt cgcgcccggc cgcgggctca ggctctcgga ggcggcggcg cggagcctcg
     2221 agcgctacca ctggcctggc aacgtccgcg aactgcgcaa cgtcatccag cgggtttccc
     2281 tcttcgcccg cgaggaggtc ggcctcgagg acctcccccc cgagatcctc gacggcaatc
     2341 ccgtcgagct gctcgcgcgc gcgtgcgccc gctgcttctc cgagcagtcc ctctccttcg
     2401 gccaggtcgt cgagtgcctc gagacgaacc tcctgcgcca ggcgctcaag gacgccgggg
     2461 gcaaccgcag ccaggccgcg cgcgccctcg ggctctcgct ctcgacgatg cgggacaagt
     2521 tgaaaaagta cggcctcgac ggcggggccg atctcgacat cgactgaccg gcctcgcgga
     2581 aatccgcggc ctttcgcggt tttccgcaga tgcggcgcaa gacgcccacg ccggcctcca
     2641 cccgccccgc tgcgcgtaat cccccgccaa ttcgcgcgat tacgcgctcg cgctttcccg
     2701 ggcctccccg ggtcgagcgc tggccggccc cttgcagttc tccggcacac acgtggccga
     2761 gtccgccgat tcccagacgc ttcttctgag gggtgaggtg atcgaggtgt ccctccggca
     2821 gggacgccgg tacctcacgg tcgcgctcga gccccactgc ctgcacgcct cgaccgggat
     2881 tcccgaggac acccacctcg gagaccgcgt ccgggtcgag gccgagatcc gggtcgtcaa
     2941 cgtcgccccc gagctgcccc acgacatccg gcagcccgcc cggcgccctg cgtgaaacgt
     3001 cgaggaggag acatgagcga accccgtccg accatctggg agcaggcccg catgagcggg
     3061 tactcccggc gcgacttcgt gcagttctgc gcgtggatgg ccgccgccgc cggcattcac
     3121 gagagcgctc tcggcgcggt cgtgcaggcg atggacacga agccgcgcct cccggtcgtc
     3181 tggttccact tccaggagtg cacgtgctgc agcgagtcgt tcatccggtc gtcgcacccc
     3241 atcgtgggcg acatcgtgct ggacaagatc tcgctcgact acacgttgac tctccaggcg
     3301 gccgcgggcc accaggcgga ggcggccctc aacgagacga tgaagaaata caagggccag
     3361 tacctcatgc tcgtcgaggg gtcggtcccg acggcggagg gcggcgtcta ctgctgcatc
     3421 gccgggcgga ccgcgctcga catcgtcaag gaagccgcgg cgaacgcgaa ggcgatcgtc
     3481 gcgtggggca gctgcgcctc gaacggctgc atccaggcgg ccaacccgaa cccgacgtcc
     3541 gcgaccccgg tccacaagct catctcgggc aagcccatca tcaacgtccc cggctgtccg
     3601 ccgatcgccg aggtcatggc cggtacggtc gtccacctcc tcgcgttcga ccgcattccg
     3661 cagctggacg ggctcgggcg cccgatggcg ttctactcgc ggcgcgtcca cgacacgtgc
     3721 taccgccgcc cgaactacga cgcgggcctc ttcgtcgaga cgttcgacga cgagaacgcg
     3781 cggcgcggct actgcctcta caaggtcggc tgccgcggcc ccgtgacgta caactcctgc
     3841 ggcgtcatcc gctggaacaa cggggtgagc tacccgatcc agtccgggca cggctgcatc
     3901 ggatgcagcg aggcgaactt ctgggacaac gggccgttct accggcacct cccgagcttc
     3961 cccggcttcg gaatcgagac gacggcggac aaggtcggcg tcgcggccgg agccgccgtc
     4021 gccgtgggca tcgcggccca cgccgtgacg acgaacatcg ccaagcgcaa gcagatccgc
     4081 gaggaaatcc acaagagcga ccggacacct gaagaggtga ccggtccgca caagtgagga
     4141 cgccatgagc aaccgcatcg tcgtcgaccc gatcacgcgc atcgaagggc acctgaggat
     4201 cgaggccgag atcaaggacg ggaagatcgt cgacgccttc agctcgggca ccatggtccg
     4261 cggcttcgag ctcatcctga aggggcgcga cccgcgcgac gcgtgggcgt tcacggaacg
     4321 ggcctgcggt gtctgcacga cggtccacgc gcttgcctcc gtccgcgccg tcgaggatgc
     4381 gctgaagatc gtggttccgc cgaacgccga gctcgtgcgg aacctcatgt tctgcgcgca
     4441 gtatctccag gaccacgtcg tccacttcta ccacctgcac gcgctcgact gggtcgacgt
     4501 cgtgagcgcc ctgaaggccg acccgaagaa gacgtccgag atcgcccaga gcatctcgaa
     4561 ctggccgaag agctcgccgg gctacttctc ggacctccag aagcggctca cggccttcgt
     4621 cgggagcggg cagctcggca tcttcgcaaa cggctactgg ggccactcgg cctacaagct
     4681 tcccgccgag gtgaacctcc tcggggtcgc gcactacctc gaggccctcg agtggcagaa
     4741 ggagatcgtc aaggtccaca cgatcttcgg cggcaagaac ccccacccga actacctcgt
     4801 gggcggcgcc ccgtgctcct tcaacatcga ggaagcgagc gcgatcaacg ccgagcgcct
     4861 cgcgtacgtc ggaaccctct tcgaccaggc cacggagttc gtcgagaagg tctacctccc
     4921 cgacctgatg gccgtcgcgt ccttctacaa ggactggggc gcgatcggcg gcggcctgcc
     4981 gaactacatg gcgtacggag accttccgac gaacggctac gcggacgtcg cgaagttcaa
     5041 gttcccccgc ggcgccatcc tgaaccggaa cctcgccgag gtcctgccga tcgacggcaa
     5101 ggacgagaag cagatcgagg agtacatctc ccactcgtgg tacgagtacg gtgccggcga
     5161 cggagccggc ctccatccct ggaagggcga gacgaagctc aggtactcgg gcccgaagcc
     5221 cccgtacgag catctcgagg tcgacaagaa gtacagttgg ctcaagacgc cgcgctggaa
     5281 gggcaatgcg atggaggtgg gcccgctggc ccgctgcctc gtcgcctacg cctccgggtc
     5341 ggccgaggtc aaggagctcg tcgacggagc cctcaagcag ctcaacgtgc ccgtcggggc
     5401 cctcttctcg acgctcggcc ggacggccgc ccgcgggctc gagacgaagc tcatcgcccg
     5461 ctgggcgaag gagttctacg gccagctcct cgcgaacatc aagaacggcg acagccggac
     5521 gttcaacggc gagaagtggg agcccgcgac gtggcccgtc gaggccaagg gcgtcggact
     5581 ctcggaggcg ccgcgggggg cgctcgccca ctggatcgtg atcaaggacc gcaagatcga
     5641 caactaccag ctcgtcgtcc cgagcacgtg gaacgcctcg ccgcgggacg ccaaggggca
     5701 gcggtcggcc tacgaggccg ccctcgtcgg cacgcccgtc gccaaccccg agcatccgct
     5761 cgagatcctc cggaccatcc actcgttcga cccgtgcatc gcctgcgccg tccacctcta
     5821 cgacgagaag ggcaaccacc tccaccaggt gaaggtcctc taggaggcgc cccatgccca
     5881 agcccaagac cgaggtccgg cgcgtctacg tctggcagct tccggtccgc ctgtaccact
     5941 gggtgaacgc cctctgcgtc ctcctcctcg cggccacggg ctacctcatc gggtcgcctc
     6001 cggccatcca gagcgcacgg gaagcgtcgt tcggctactg gttcggcacc gtgcggttca
     6061 tccacttcct gtccgccttc gtcttcttct tcaacttcgt cttccggatc tactggggct
     6121 tcgtcggcaa cgcctgggcc cgctggccgc agttcctccc gttccggaag gtgcagcgcg
     6181 aggaggccat cgaggtcctg aaggtggaca tcctccaggc caaggccgcc ccgctcccgt
     6241 cgatcggcca caacccgatc gcgggattca cctacttcct cacgttcctc gcgttcctgt
     6301 tccagagcgt gacgggattc gggatgtacg cggcgatgag ccacgcgaag ctgccgcacc
     6361 tcttcgcgtg gatcgtgccg ctgatggggg gcgacttcgc ggtgcgccag tggcaccaca
     6421 tgacgatgtg gttcttcatc ctcttctcga tggtccacgt ctatctcgtc ttctaccacg
     6481 actacgtcga agcgcgcggc gttctctcgt ccatggccgg aggctggaag ttcatcgaga
     6541 ggccagcgga aaagtgagca acgaccggcc ggacgaggcg gcccggccgg tcctggtcct
     6601 ggggatcggc aacgtcctgc tcggggacga gggcgtggga gttcacgccg tgcggcggat
     6661 ggaaccggaa acgtggccgc caaacgtgaa gctcctcgac ggcggcacgg gcggcttcca
     6721 tctcctctcg ctcttccagg agtacgaacg gatcgtcctc atcgacgcga cgatggacgg
     6781 ccagccgccc ggcacggtcc ggctcatccg gcctcgcttc gcctcggact atccccggac
     6841 gctcagcgcc cacgacatcg gcctcaagga cctcgtcgag tcggccgccc tccttggcct
     6901 cgagcccgag gtcgtcctcg tcgtcgtctc cgtggagaca cttcccgagg gcctccacgc
     6961 cgagctgagc cccgaggtcg cggccgcgct gccgcgggtc gccgcgctcg cgcg
//
LOCUS       DAOMFY010000209         6957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3528_length_6957_cov_7.230803, whole genome shotgun sequence.
ACCESSION   DAOMFY010000209 DAOMFY010000000
VERSION     DAOMFY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3528_length_6957_cov_7.230803"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLB01_14565"
     CDS             complement(<1..733)
                     /locus_tag="PLB01_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78575.1"
                     /translation="MAAQQGRVEERFSERASSSILLVYDGQRLEPGVERSLLAALERA
                     YAELVQDLGSAPPRKVEVVVYAERDWRLATQAPDWSGGLFDGKVRVPAQGLTGVTPQL
                     MAVLKHEMTHAFVAARTGGRAPVWLHEGLAQVEEGKTAGPWAAALLDAWRREKTFGLS
                     SLEGSFRRFSAGQVRTSYVVALAAVEMLRDTRGPGAIGHLLDHLGDGTPVEEAMRGTV
                     RGDYSRVESDLADWLVRALKVPFREA"
     gene            814..2022
                     /locus_tag="PLB01_14570"
     CDS             814..2022
                     /locus_tag="PLB01_14570"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQQ78576.1"
                     /translation="MKRKNFLRVVPSAGARIAALLALFVGVAPQGAAAEEAKRQTTAE
                     PSPSSPPKKSPSTSSALSSALARIAETAVRDGRTAGISFVVTKGGAVLASGAYGFADL
                     ENRVPASVGTVYAIGSLTKTFTAAAVLALADDGKLSLDDPIGTFVPSFPEPGRSATVR
                     QLLNHTSGIRSMTSLGPRYWAQAGREIEPADLVAIFANEPPDFPPGTAYRYSNSGYVL
                     LGLVVEKASGMPWGSFVTKRFVVPLGLSGTRDGQTAALVPGRARAYSRTKAGGFENAW
                     HVSLTQGYAAGALLSTAPDVAAWIRRLSAEPPVNAASARAMTTPGVLPDGKPLSYGYG
                     IGIDTFAGRRRLFHGGGLPGFDAWAAYVPGDDLAVAVLCNTDGDAAMEVADAMAGLVL
                     GAPGARTQRP"
     gene            complement(2030..3010)
                     /locus_tag="PLB01_14575"
     CDS             complement(2030..3010)
                     /locus_tag="PLB01_14575"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HQQ78577.1"
                     /translation="MSLRAALAAALMLPAALTPAAEPSPAVSEVAGLLPGVFVSRDPS
                     ASIRFSVAAVPKNRIAADAPVFYLEAAKSARPDAPFLQRFLKLEAAGAVVAVRVYEPR
                     DLAGVRGKWREPEALAVFTERDVRERPDCRMTLKKAPAGHWTGGTAAATCFSPAAGAA
                     VAVAMSLSPQTLEWREDGKSAAGEISIFGRQRETAALAAPVTLGTGRDGGFSMKVKEE
                     ASVQGPQALEVISPSSPSKKYDLAELRAIAGSDSLSLSRLIQAPEASAAAVVVVASSS
                     GALSVFSFAEISSSALSPSLDVSGAAPRLVAAPGRGLDDVVSIELRVFDR"
     gene            complement(3007..4848)
                     /gene="typA"
                     /locus_tag="PLB01_14580"
     CDS             complement(3007..4848)
                     /gene="typA"
                     /locus_tag="PLB01_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQQ78578.1"
                     /translation="MSGIRNIAIIAHVDHGKTTLVDCLLKQSGTFRANEAVSGTERIM
                     DSNDLEKERGITILAKNTSIRYHGTKINIVDTPGHSDFGGEVERVLKMVDAVLLVVDA
                     AEGTMPQTRFVLRKAFELGLKPIVVVNKIDRPDARPHQVIDEVFDLMVDLGATDEQLD
                     FPIVYTSAKHGFARREVDHAESDVRPLLDAILVEVPEPGGNPAAPLQLLVASLDYDNY
                     LGRLLIGRIVNGTIRPGQQVSVCKLDGTITKGKVAKVMAFEGLRRAEVEEAQAGDLVV
                     ISGIDEVTIGETLADLENPVALAPINVDEPTVSMMFRVNDSPFAGREGKYVTSRNIGE
                     RLQKELRTNVAMRVEETDSPDAFRVSGRGELHLAITVETMRREGFEFALSRPQVILKE
                     IDGKQCEPIEHLVVDVPETAMGTVIEKLGQRKAEMTNMTNHGSGRVRIDFHIPTRGLF
                     GYRNEFMTDTKGEGLLYHNFHGYEPYKGEVTGRGRGAMVCKEAGETTAHSLERLEERS
                     TLFLPPGVEVYGGMICGESARPNDLVVNPCVKKHLTNMRSSGSDFAIRLTPPRLMPLE
                     LAIEWIEDDELVEVTPKSIRLRKRILDHSMRRVSEKRAGKIEEEDEE"
     gene            4974..6440
                     /locus_tag="PLB01_14585"
     CDS             4974..6440
                     /locus_tag="PLB01_14585"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ78579.1"
                     /translation="MTRAARLALTFIEISLCVFLTVLLGRGFQARGLPDLKPWHRADL
                     GDVRAGELPETATLADFLKREDAVFAALKEKVLSAVPEADRTAGNRYLAGGPLDPTRL
                     GAADGNRTFECVPEKIGGGALLLHGLTDGPYSLRPIAETLRRLGYYTLAPRMPGHGTA
                     PAGLMSAEWPDWMSVVRLAARHVRATAGAGRPFVVVGYSNGGALAVKYAQDVLEGSGL
                     PRPDRIVLMSPMIGVTPFAGFARVAMLLHRIPYFEKNAWLDVVPEYNPYKYNSFPANA
                     GLQTYRLTQALNAQTNRLAAGGRLKDLAPVLAFQSLVDATVSTEAIVHRFFDRLPANG
                     SELVLFDVSRESTLAPFITPPEEKLLTDLRARTDLPYRLTAVTNARRDTRDVVARSWG
                     PGPARFEEAALGLAWPPDVFSLSHVAIPFPESDDLYGRLAEPPPTFGIRLGRIGPRGE
                     RAVLTVPFDQWMRLTWNPFFPYLAERVSAWAAISSSAR"
     gene            complement(6418..>6957)
                     /locus_tag="PLB01_14590"
     CDS             complement(6418..>6957)
                     /locus_tag="PLB01_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78580.1"
                     /translation="AATPAAVPAPTPAGATAPQPTGTAAAAAPAWKPFQELAFLTGSW
                     AGSANLGPRIGGRVARFGPELSGAFFVVRGSTFIAADEGGAEETLEEEGWFAYDREKR
                     KYVATWLFSNGVVGAFDVELLPDGVRLLSRELVNYESGTRARMLFQRRPEGDVSMNVD
                     LALPGKDFVPWLVSALKKK"
BASE COUNT         1012 a         2481 c         2391 g         1073 t
ORIGIN      
        1 gcgcctcccg gaacggcacc ttcagcgccc gcacgagcca gtccgcgaga tccgattcca
       61 cgcgcgaata gtcgccgcgc acggtcccgc gcatggcctc ctcgacgggc gttccgtctc
      121 cgaggtggtc gagcaggtgg ccgatcgcgc ctggcccgcg cgtgtcacgc agcatctcga
      181 ccgcggcgag ggccacgacg tagctggtcc gcacctgtcc ggccgagaag cgccggaacg
      241 agccctcgag cgacgacagg ccgaacgtct tctcgcgcct ccacgcgtcg aggagcgcgg
      301 cggcccaggg tccggccgtc ttcccctcct cgacctgcgc gagaccctcg tggagccaga
      361 ccggagcgcg cccgccggtc cgcgcggcga cgaaggcgtg cgtcatctcg tgcttgagca
      421 cggccatgag ctgcggcgtg acgcccgtga ggccctgcgc agggacacgg acctttccgt
      481 cgaagagtcc gccggaccag tccggcgcct gcgtcgcgag ccgccagtcc cgctcggcgt
      541 agacgacgac ctcgaccttg cggggcggag ccgacccgag gtcctgcacg agttccgcat
      601 acgcgcgctc gagcgccgcg agaagggaac gctcgacgcc gggctcgagc cgctggccgt
      661 cgtagacgag gaggatgctc gagctcgcgc gctcggagaa ccgctcctcg acgcgtccct
      721 gctgtgcggc cacggacgcg gtcgcgagac acaccagggc cgcaaacgcc aagggggttc
      781 tcgcgcggca cggcatgcgt tcatgatagg ccgatgaaga ggaagaattt tcttcgagtg
      841 gtcccgagcg cgggcgcgcg gatcgccgcg cttctcgcac tgttcgtcgg ggtcgcgccg
      901 cagggggccg ccgccgaaga agcgaagagg cagaccaccg ccgagccatc tccctcttca
      961 cctccgaaga aatctccctc tacctcttcg gccctctctt cagccctcgc ccgcatcgcc
     1021 gaaacggcag tcagggatgg aaggactgcc ggcatctcat tcgtggtgac gaagggcggc
     1081 gccgtcctcg cctccggggc gtacgggttc gcggatctcg agaaccgtgt gcccgcgtcc
     1141 gtcggcacgg tctatgcgat cggctcgctc acgaagacgt tcacggccgc ggccgtcctc
     1201 gccctcgccg acgacgggaa gctctcgctc gacgatccga tcgggacgtt cgtcccgtcc
     1261 ttccccgagc ccggccggag cgccaccgtc cgccagctcc tgaaccacac gtcgggaatc
     1321 aggagcatga cgagcctcgg cccgcgctac tgggcgcagg cggggcggga gatcgaaccg
     1381 gcggacctcg tcgcgatctt cgcgaacgag ccgcccgatt tcccgccggg aacggcgtac
     1441 cggtacagca attccggata cgtcctcctc ggcctcgtcg tggagaaggc gtccgggatg
     1501 ccttggggca gcttcgtgac gaagcgtttc gtcgtcccgc tcggcctctc cgggacgcgc
     1561 gacggacaga cggccgcgct cgttcccggc cgcgcccgcg cctattcgcg aacgaaggcc
     1621 ggcggtttcg agaacgcctg gcacgtgagc ctgacgcagg gctacgccgc cggcgcgctc
     1681 ctctccaccg ctccggacgt cgcggcatgg atccgccggc tctccgccga gccgcccgtg
     1741 aacgccgcct cggcccgcgc gatgacgacg cccggcgtcc tgccggacgg caagcctctc
     1801 tcctacgggt acggcatcgg catcgacacg ttcgcaggcc ggaggcgcct cttccacggc
     1861 ggcggactgc cgggcttcga cgcgtgggcc gcgtacgtcc ccggcgacga cctcgcggtc
     1921 gcggtcctct gcaacacgga cggcgacgcc gcgatggagg tcgccgacgc gatggcgggc
     1981 ctcgtcctcg gcgcgcccgg cgcgaggacg cagcggccct gagccggact cagcggtcga
     2041 agacgcgaag ctcgatcgag acgacgtcgt cgaggccgcg cccgggcgcc gcgacgaggc
     2101 gcggcgcggc gccggagacg tccagggaag gagaaagagc ggaagaagag atttctgcga
     2161 aggagaatac ggaaagagct ccgctgctcg aggccacaac gacgacggcc gccgcggaag
     2221 cctccggagc ctggatgagc cgcgaaagcg acaggctgtc gcttccggca atggcgcgga
     2281 gctccgcaag gtcgtatttc ttcgaaggtg aagacgggct gatcacttcg agcgcctgcg
     2341 gcccctgaac cgaggcctcc tctttcacct tcatagaaaa gccaccgtca cgacctgtcc
     2401 caagcgtcac aggcgcggcg agggcggcgg tctcccgctg ccttccgaag atcgagatct
     2461 ctccggcggc gctcttcccg tcttcccgcc actcgagcgt ctgcggcgag agcgacatcg
     2521 cgaccgcgac cgccgcgccg gcggccggcg agaagcacgt cgccgcggcc gtgccgcccg
     2581 tccagtggcc ggccggcgct ttcttgagcg tcatgcggca gtcgggccgc tcgcggacgt
     2641 cccgctccgt gaagaccgcg agggcttccg gctcgcgcca cttgccgcgc acgcccgcga
     2701 ggtcccgcgg ctcgtacacg cggaccgcga cgaccgcgcc ggctgcttcg agcttcagga
     2761 agcgctggag gaacggggcg tcggggcgcg cggacttcgc ggcctcgaga tagaacaccg
     2821 gcgcgtcggc cgcgatcctg ttcttcggaa cggcggcgac tgagaaacgg atgctcgcgg
     2881 aggggtcgcg cgacacgaag acgcccggga ggaggcccgc gacctcggag accgcgggcg
     2941 agggctcggc cgccggcgtg agggcggcgg gaagcatcag cgcggcggcg agcgccgcgc
     3001 gcaggctcac tcctcgtcct cctcctcgat cttgccggcg cgcttctcgg aaacccgccg
     3061 catcgagtgg tcgagaatac gcttcctcag gcggatggac ttcggcgtga cctcgacgag
     3121 ctcgtcgtcc tcgatccact cgatcgcgag ctcgagcggc atgaggcgcg gcggcgtgag
     3181 gcggatcgcg aagtccgagc ccgacgaccg catgttcgtg aggtgcttct tgacgcaggg
     3241 gttcacgacg aggtcgttgg gacgggcgct ttcgccgcag atcatcccgc cgtagacctc
     3301 gacgccgggc ggaaggaaca gcgtcgagcg ctcctcgagc cgctcgaggg aatgcgccgt
     3361 cgtctcgccg gcctccttgc acaccatggc gccgcggccg cggcccgtca cctcgccctt
     3421 gtagggctcg tagccgtgga agttgtggta caggaggccc tcgcccttcg tgtccgtcat
     3481 gaactcgttg cgatagccga agaggccgcg cgtcgggatg tggaagtcga tcctcacgcg
     3541 tccggagccg tggttcgtca tgttcgtcat ctcggccttg cgctggccga gcttctcgat
     3601 gacggttccc atcgcggtct cggggacgtc gacgacgagg tgctcgatcg gctcgcactg
     3661 ttttccgtcg atctccttca ggatgacctg cgggcgcgag agggcgaatt cgaagccctc
     3721 gcggcgcatc gtctcgaccg tgatcgcgag gtggagctcg ccacggccgg agacgcggaa
     3781 cgcgtcgggg ctgtccgtct cctcgacgcg catcgccacg ttcgtgcgga gctccttctg
     3841 gaggcgctcg ccgatgttgc ggctcgtgac gtacttgccc tcgcggcccg cgaacggcga
     3901 gtcgttgacc cggaacatca tggagacggt cggctcgtcg acgttgatgg gcgcgagcgc
     3961 gacggggttc tcgaggtcgg ccagcgtctc gccgatcgtc acctcgtcga tgcccgagat
     4021 gacgacgagg tcgccggcct gcgcctcctc gacctcggcg cgccggaggc cctcgaacgc
     4081 catgaccttc gcgaccttgc ccttcgtgat ggtcccgtcg agcttgcaga ccgagacctg
     4141 ctgcccgggc cggatcgtcc cgttcacgat gcggccgatg aggagccggc cgaggtagtt
     4201 gtcgtagtcc agcgaggcca cgaggagctg gagcggagcg gcggggttgc ctccgggctc
     4261 gggcacctcg acgaggatcg catccaggag aggcctgaca tccgactcgg cgtggtccac
     4321 ctcgcgccgc gcgaacccgt gcttggcgga cgtgtacacg atcgggaagt cgagctgttc
     4381 gtccgtagcg ccgaggtcca ccatgaggtc gaagacctcg tcgatgacct ggtgcgggcg
     4441 cgcgtccggg cggtcgatct tgttcacgac gacgatcggc ttgaggccga gctcgaacgc
     4501 cttgcggagg acgaagcgcg tctgcggcat cgtgccctcc gccgcgtcca cgacgaggag
     4561 gaccgcgtcc accatcttga ggacgcgctc gacctcgccg ccgaagtcgc tgtgccccgg
     4621 cgtatcgacg atgttgatct tcgtcccgtg gtaccggatc gaggtgttct tcgcgaggat
     4681 cgtgattccg cgctctttct cgaggtcgtt gctgtccatg atccgctcgg tgcccgagac
     4741 ggcctcgttc gcgcggaacg ttccggactg cttcaggaga cagtccacga gcgtggtctt
     4801 gccatggtcg acgtgcgcga tgatcgcgat gtttcggatt ccggacatgg ggcctgcttt
     4861 ccgagcgcgg ggccgggcgg cggcctcttc gcggggctgt gcaggatatc agagaagacg
     4921 ggagagaaga ttttcttcga atgagtaaga gcgcgatacg ctttgcgcgt tccatgacga
     4981 gagccgcgcg gcttgcgctc accttcatag aaatctccct ctgtgtcttt ctcacggtcc
     5041 tcctcggccg cggattccag gcgaggggcc tgccggacct caagccctgg catcgcgccg
     5101 acctcggcga cgtccgggcc ggcgagctgc ccgaaacggc gacgctcgcc gatttcctga
     5161 agcgcgagga cgcggtcttc gctgcgctca aggagaaggt gctctccgcc gtccccgaag
     5221 cggaccgcac ggccgggaac cggtatctcg cgggcggccc tctcgacccg acgcgtctcg
     5281 gcgccgcgga cggcaaccgg acgttcgagt gcgttcccga gaagatcggc ggcggcgcgc
     5341 tgctcctcca cggcctcacg gacgggccct acagcctcag gccgatcgcc gagacgctcc
     5401 gcaggctcgg ctactacacc ctcgcgccgc gcatgcccgg ccacggcacc gcgcccgcag
     5461 gcctcatgtc cgcggagtgg ccggactgga tgagcgtcgt ccgcctcgcg gcgcggcacg
     5521 tgcgcgcgac ggcgggcgcc ggcaggccgt tcgtcgtcgt cggttactcg aacggcggcg
     5581 cgctcgccgt caagtacgcg caggacgtcc tcgagggctc gggcctcccg cggccggacc
     5641 gcatcgtcct gatgtcgccg atgatcggcg tgacgccctt cgcgggcttc gcgcgcgtcg
     5701 cgatgctcct ccaccgcatc ccgtacttcg agaagaacgc gtggctcgac gtcgtccccg
     5761 aatacaaccc gtacaagtac aactcgtttc ccgcgaacgc cgggctgcag acgtaccggc
     5821 tcacgcaggc gctgaacgcg cagaccaacc gcctcgcggc gggcggccgg ctcaaggacc
     5881 tcgcgcccgt cctcgcgttc cagtcgctcg tcgacgcgac cgtctcgacg gaggcgatcg
     5941 tccaccggtt cttcgaccgg ctgcccgcga acgggagcga gctcgtcctc ttcgacgtca
     6001 gccgggagag cactctcgcc cctttcatca cgccgccgga ggagaagctc ctcacggacc
     6061 tgagggcgcg gaccgacctc ccgtaccgcc tcacggcggt cacgaacgcc cggagggaca
     6121 cgcgcgacgt cgtggcccgg tcctgggggc ccggaccggc gcgattcgag gaggcggcgc
     6181 tcggcctcgc gtggcctccg gacgtcttct cgctctcgca cgtcgcgatc ccgttcccgg
     6241 agagcgacga cctttacggc cgcctcgcgg agcctccgcc gacgttcggc atccggctcg
     6301 ggcgcatcgg gccgcgcggc gaacgcgcgg tcctcaccgt cccgttcgat cagtggatgc
     6361 gcctgacgtg gaacccgttc ttcccgtacc tcgccgagcg cgtctcggcc tgggcggcta
     6421 tttcttcttc agcgcgctga cgagccaggg cacgaagtcc ttcccgggaa gcgcgagatc
     6481 gacgttcatc gagacgtcgc cctcggggcg ccgctggaag agcatccgcg cgcgcgtgcc
     6541 cgactcgtag ttcacgagct cgcgcgagag cagccggact ccgtcgggca gaagctcgac
     6601 gtcgaaggct cccaccaccc cgttcgagaa cagccaggtc gccacgtact tccgcttctc
     6661 gcggtcgtag gcgaaccagc cttcctcctc cagggtctcc tcggcccccc cctcgtcggc
     6721 ggcgatgaag gtgcttcccc ggacgacgaa gaacgcgccc gagagctcgg ggccaaaacg
     6781 cgcgacgcgt ccgccgatcc gggggccgag gttcgcgctt cccgcccagg atccggtcag
     6841 gaaagcgagc tcctggaatg gcttccaggc gggagcggcc gccgcggcgg tccccgttgg
     6901 ctggggcgcc gtcgcccccg ccggggtcgg cgccgggacg gcggccggcg tggctgc
//
LOCUS       DAOMFY010000210         6936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3542_length_6936_cov_9.483796, whole genome shotgun sequence.
ACCESSION   DAOMFY010000210 DAOMFY010000000
VERSION     DAOMFY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3542_length_6936_cov_9.483796"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLB01_14595"
     CDS             complement(<1..364)
                     /locus_tag="PLB01_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78581.1"
                     /translation="MLLALVVATALEAAPAQMVELTPVANAGAPVSAARRSLSDVGRE
                     LREGRKAKGGFSAVETTVPREPVDLRFVSWEEDEARGEPEVVPEPQPPGIVSNEIYGG
                     WGGGGWAPVPPRRRPPHAS"
     gene            532..2562
                     /locus_tag="PLB01_14600"
     CDS             532..2562
                     /locus_tag="PLB01_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78582.1"
                     /translation="MRPRHRASALVAVAALSALGCAAIGGVRSEDGSKSAASRELAPD
                     SSTLFVPVVLSSSGLQGAFFTSEMILTNRGTTPASIVYTYVAASGGGSGSGADTLAAG
                     EQRVVADAISYLRTKGVPIPDSGNRVGTLRVAFTGLSSAAAAAVTVRTTTLVPPAAPT
                     GRAGLAYAGVAPSQLLTGTAYLCALRENASDRTNVAIQNAGDADVRIRLTFLPGDGSS
                     TTGSVEDTLPPGGFKQYRITDVAAGAANGFIRAERISGTGPYYAYAVINDNATSDGSF
                     VTPLVESSLRSRTGLTLPVVVETGTFSTEVVLTNFSGASKQVTLAYVSDAVSTADKTA
                     TLTLTVSPGEQRILPAYVQLLRDNGVPGVGPAGPTYTGSLFATVAGGDVGGLFLGART
                     SNPGGGGRYGLFYAARPFGAAVTTEAWLYGLQQNPENRTNVALISTGEVDSNPITLRV
                     EIFDGGSGAKVATIEGDPTTTVARRGFKQIGAILSQYAAGVSQAYARVTRTSGQNGFI
                     VYAVVNDGGSPGERSGDGAFVALDTPTAVAATFAGTWRNTTFGSTGSASITITGDSYT
                     QAYSGTSSVGGNVFGGAPPPPEIYANIFTPAGGTFTTHSNFYGDVTMTVLPNGTFTLT
                     AENIPNANVSRATCSGTITPPGPNAQTISATGTIFFRAGGSASTVTTLTRQP"
     gene            complement(2580..3074)
                     /locus_tag="PLB01_14605"
     CDS             complement(2580..3074)
                     /locus_tag="PLB01_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQQ78583.1"
                     /translation="MISPTMQDALNAQINSEQYSAQLYLAMSAHCATKSFKGFAHWLR
                     IQASEETAHAAKLIQFLLDRAGRLQLRPIPAPPAEFGTVIEVFEKTLEHEKAVTAKIS
                     ALFDLARKEGDYASEITLQWYVTEQVEEEATISEIVDQLHAIGAAGGAIWYLDSRMGK
                     RAQS"
     gene            complement(3093..3917)
                     /locus_tag="PLB01_14610"
     CDS             complement(3093..3917)
                     /locus_tag="PLB01_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78584.1"
                     /translation="MTGPTAAALVLLFAAAPPLPFAARVDGARAVERARYAFVLDAAK
                     PFDDVYPRSVFEKMVTREAAEEDVLRREFGMEVTRELLAAEYDRIEKETRAPDQWEAM
                     KKALGNDRGRVEQVVCRPLLVDRALRARFAFDAKIHAAERQKARDARNAFLGGGKPAG
                     AERIRLSRRASRAGSTDDMLAKARADAAGPKLLSPGAPKTTDDGAPLPVDPEMAQALE
                     KELKAKGDVTTILEERGRFSVFRLVTAAADEWLVDGVRVPKRDFEKWIESRIGTAR"
     gene            complement(3935..5737)
                     /locus_tag="PLB01_14615"
     CDS             complement(3935..5737)
                     /locus_tag="PLB01_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78585.1"
                     /translation="MLVITLLTPLALGDEISNWAAPPYWMPPGGAASQPANRDALAGR
                     QPLAGGPNALPFISLFPCRLVDTRAPVFPAPLGGGWLPPATVRSYTLAGVCNVPANAQ
                     AISLNATVVKPTGPGFLTLYPEGGAFPPVSTLNYLGNDIIVNAAVVPLSVTGGISVVL
                     GVSGGDVILDTNGYYATVPSVTSVNTLAGDLTLAAGSNVTITPSAQTLSIAAVMPSGA
                     AVLGKPGDTTLIGQGFTEIVPMDREVWTPTSLTNAPAGRFWHCSVWTGTKMFVWGGQD
                     ATQVPIYNSGGLYDPATDSWAATSTGTNVPSARRYAHCVWTGSEVLVWGGEDASLAAL
                     NTGGRYNPVANTWAAMTTASAPAGRFSAAVVWAAEASPPRMLVWGGLDRFSGSNVYPS
                     VGGAYNPASDTWSALSTGSQPAPRAAPVFVWTGAKLVLWGGYIGAGTCLDTGGRYDPA
                     SDTWAGTAAAGLGARYAGSAVWTGTRMIVWGGTCGAPHLGNGALYDPVGDAWTAMTNT
                     GAPTARYNQVAVWTGSRMIVWSGMTGGGVTNTGGIYDPAGNTWRTMTTTNAPVANYGY
                     TGHWTGLPLNEMVVWGGYGQVGGTYRTLSLFIKN"
     gene            5868..>6936
                     /locus_tag="PLB01_14620"
     CDS             5868..>6936
                     /locus_tag="PLB01_14620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ78586.1"
                     /translation="MDQRRTPLLKSFPAVFWVANVMELFERAAYYGLNSVLAVYLSNP
                     AAKGGLGFPEQSVGFLQSLIYACNYVVPIAGGALADRYGYRRMLLFAFSMLATGYFAA
                     GNVSSYGVVFAALLLMATGGGLFKPIISGTIARSTNDENSGFGFGVYYWMINVGAFLA
                     PLVVSVLKGFSWRWVFMASSGYCALMLLPALFAFKEPPRPENTKSFGDVVRGAALVLG
                     DARFMLMIVVYSGFWILYFQNFGSVLWYLRDFVDGTPVDRLFASFGIPLKFDSEHVTV
                     INAGTIILLQIVVSRVVKNVRALPTMVSGIAFGALGFLCLALSRNVWAFILGLAVFSI
                     GEMTAHPKYYSYVGLVAPQDKK"
BASE COUNT          983 a         2487 c         2335 g         1131 t
ORIGIN      
        1 ggcttgcgtg cggcgggcgg cgccgcggtg ggaccggcgc ccatcctcct ccgccccatc
       61 ctccgtagat ctcgttcgag acgatgccgg gcggctgcgg ctcggggacg acctcgggct
      121 cgccgcgggc ctcgtcttcc tcccacgaga cgaaccgcag gtcgacgggc tcgcggggca
      181 ccgtcgtctc gacggccgag aaccccccct tcgccttccg gccctcccga agctcgcgcc
      241 cgacgtccga gaggctgcgc cgggcggcgc tgacgggagc gcctgcgttc gccaccggcg
      301 tcagctcgac catctgcgcc ggggcggctt cgagcgcggt cgcgacgacg agcgccagca
      361 acatggtttt cccccgtgcc ggaaggatag tccccggcag acctcttcgc cgccccctgt
      421 cgtccggacc gatgcggatc gccgtttttc gtttttcttt tggcccgcac tccctgagaa
      481 tctgtctctg cctcgagtcc cgcgtggaat gcgaagaggg gaggaaccgt catgcggcct
      541 cgccaccgtg cttcggctct cgtcgcagtt gctgcacttt ccgcactcgg ctgcgccgcg
      601 atcgggggcg tccgatcgga ggacggctcg aagtcggccg cgagccgcga gctcgcgccc
      661 gactcctcga cgctcttcgt cccggtcgtc ctctcttcgt cgggcctgca gggtgcgttc
      721 ttcacctcgg agatgatcct cacgaaccgc ggcacgacgc cggcgtcgat cgtctacacg
      781 tacgtcgccg cgtcgggcgg cgggagcggc tccggcgccg acacgctcgc ggccggcgag
      841 caacgggtgg tcgccgatgc gatctcgtat ctccgcacga agggagtgcc gatccccgac
      901 tccggcaacc gcgtcggcac cctgcgcgtc gcgttcacgg ggctctcgtc cgccgcggcg
      961 gcggccgtca cggtccggac gacgacactc gtgccgcccg cggcgcccac gggccgggcg
     1021 gggctcgcgt acgccggcgt cgcgccttcg cagctcctca cgggcaccgc gtacctctgc
     1081 gcgctgcgcg agaacgcgtc cgaccggacg aacgtggcca tccagaacgc cggggacgcc
     1141 gacgtccgca tccggctgac gttccttccg ggcgacggct cgtcgacgac gggctcggtc
     1201 gaggacacgc tcccgcccgg cggcttcaag cagtaccgga tcaccgacgt cgcggcgggc
     1261 gccgcgaacg gtttcatcag ggccgaacgg atctccggga ccgggcccta ctacgcctat
     1321 gcggtcatca acgacaacgc gacgtccgac ggctccttcg tgacgccgct cgtggagagc
     1381 tcgctccgga gccgcacggg cctcacgctc cccgtcgtcg tcgagacggg cactttctcc
     1441 accgaggtcg tcctcacgaa cttctcgggc gcgtcgaagc aggtcacgct cgcctacgtc
     1501 tccgatgccg tgagcacggc cgacaagacg gccacgctca cgctgacggt ctcgccggga
     1561 gagcagcgga tcctgcccgc ctacgttcag ctcctgcggg acaacggcgt ccccggcgtc
     1621 ggccctgcgg gccccaccta cacgggatca ctcttcgcca cggtcgccgg cggcgacgtc
     1681 ggcggactct tcctcggcgc ccgcacgtcg aaccccggag gcggcggacg gtacgggctc
     1741 ttctacgccg cgaggccgtt cggcgccgcg gtcacgacgg aggcgtggct ctacggtctt
     1801 cagcagaacc cggagaaccg gacgaacgtg gcgttgatct cgacggggga ggtcgattcg
     1861 aaccccatca cgcttcgcgt cgagatcttc gacggcggga gcggcgccaa ggtcgccacg
     1921 atcgaagggg atccgacgac gacggtcgcg cggcgcggct tcaagcagat cggcgccatc
     1981 ctgagccagt acgcggccgg ggtctcccag gcttacgccc gcgtcacccg gacgagcggc
     2041 cagaacggat tcatcgtcta cgccgtcgtg aacgacggcg ggagtcccgg cgagcggagc
     2101 ggcgacggcg ccttcgtggc gctggacacg ccgacggcgg tcgccgccac gttcgcgggc
     2161 acgtggcgga acaccacgtt cggcagcacg ggcagtgcgt cgatcacgat cacgggcgac
     2221 tcgtacacgc aggcgtactc cggcacctcc agcgtgggcg gcaacgtgtt cggcggcgcg
     2281 ccgccgccgc ccgagatcta cgcgaacatc ttcacgccgg ccggcgggac gttcacgacg
     2341 cactcgaact tctacggcga cgtcacgatg acggtgctgc cgaacggaac gttcacgctg
     2401 accgcggaga acatcccgaa cgcgaacgtg agccgcgcga cgtgctcggg gaccatcacg
     2461 cctcccggcc cgaacgccca gaccatctcg gccaccggga cgatcttctt ccgcgcggga
     2521 ggaagcgcga gcaccgtgac gacgctgacc cggcagccgt gagacggcct ggcccgtgat
     2581 caggactgcg cgcgcttgcc catccgggaa tcgaggtacc agatggcgcc ccccgctgcg
     2641 ccgatcgcgt ggagctggtc gacgatctcg ctgatcgtcg cctcctcctc cacctgctcg
     2701 gtgacgtacc actggagggt gatctcgctg gcgtagtccc cttccttgcg agcgaggtcg
     2761 aagagggcgg agatcttggc ggtgaccgcc ttctcgtgct cgagcgtctt ctcgaagacc
     2821 tcgatgaccg tcccgaactc ggccggcggc gccgggatcg ggcggagctg caggcggccg
     2881 gcgcggtcga ggaggaactg gatgagcttg gccgcgtggg ccgtttcctc ggacgcctgg
     2941 atccggagcc agtgcgcgaa tcccttgaag ctcttcgtgg cgcaatgcgc gctcatcgcg
     3001 agatagagct gggccgagta ctgctcggaa ttgatctgtg cgttcagggc gtcctgcatc
     3061 gtcggactga tcatgaatcc ccccgcggaa gtctagcgtg ccgttccgat gcgcgactcg
     3121 atccacttct cgaagtcgcg cttcggaacc ctcactccgt cgacgagcca ctcgtcggcg
     3181 gctgccgtga cgaggcgaaa gacggagaag cggccgcgct cctcgaggat cgtcgtgacg
     3241 tcgcccttcg ccttcagctc cttttccagg gcctgggcca tctcgggatc gacgggcaga
     3301 ggagcgccgt cgtccgtggt tttcggggcg ccgggtgaga ggagcttcgg cccggccgcg
     3361 tcggctcgcg cctttgccag catgtcgtcc gtgctgccgg cgcgcgaggc ccggcgcgag
     3421 agccggatcc gctccgcgcc cgccggcttc ccgcctccga ggaaagcgtt tcgcgcgtcg
     3481 cgcgccttct ggcgctctgc cgcgtggatc ttcgcgtcga aggcgaatcg ggcccgaagc
     3541 gcccgatcga cgagaagcgg ccggcagacg acctgctcga cccggccgcg gtcgtttccg
     3601 agcgccttct tcatcgcctc ccactggtcc ggggcgcggg tctccttctc gatgcggtcg
     3661 tattcggccg cgaggagttc gcgcgtcacc tccatcccga actcgcggcg gaggacgtcc
     3721 tcctcggccg cctcgcgcgt caccatcttt tcgaagacgc tcctcggata gacgtcatcg
     3781 aagggtttcg cggcgtcgag gacgaaggcg tagcgcgccc tctccaccgc ccgcgcgccg
     3841 tcgacacgtg cggcaaacgg aagcggcggc gccgccgcga agagaaggac gagcgccgcc
     3901 gcggtaggtc cagtcattca gccgaaagcc tgcctcagtt cttgatgaag agcgagagag
     3961 tccgataggt gccgccgacc tgtccgtatc cgccccagac gaccatctcg ttcagaggga
     4021 gacccgtcca gtgtcctgtg tacccgtagt tcgccacggg tgcgttcgtc gtcgtcatcg
     4081 tccgccacgt gtttccggcc gggtcgtaga tgccgccggt gttggtcacg ccgccacccg
     4141 tcattccgga ccagacgatc atccgcgagc cggtccagac ggcgacctgg ttgtaccggg
     4201 ccgtcggagc gcccgtattc gtcatcgcgg tccacgcgtc acccaccggg tcgtagaggg
     4261 cgccgtttcc gaggtgtggc gcgccgcagg tcccgcccca gacgatcatc cgcgtgcccg
     4321 tccagacggc gctcccggcg taacgcgcgc ccaggcccgc cgccgccgta ccagcccacg
     4381 tgtccgacgc cgggtcgtat cgtccgccgg tgtccaagca cgtcccggcg ccgatgtacc
     4441 ccccccagag gacgagtttc gccccggtcc agacgaagac gggcgccgcc cgcggggccg
     4501 gctgactccc cgtgctgaga gcggaccagg tgtcgctggc cgggttgtag gcgccgccga
     4561 ctgagggata gacgttggaa cccgagaagc ggtcgagccc tccccaaacg agcattcgag
     4621 ggggcgaggc ctcggcggcc cagacgacag cggccgagaa ccgaccggcc ggcgcggagg
     4681 ccgtcgtcat ggctgcccac gtgttcgcga ccgggttgta ccggcctccg gtgttcaggg
     4741 cggcgaggga cgcgtcctca cctccccaga cgagcacttc gcttcccgtc cagacgcagt
     4801 gggcgtagcg cctggcggaa ggaacgttcg tgcccgtgct cgtcgccgcc cagctgtccg
     4861 tcgcgggatc gtagaggccg cccgagttgt agatggggac ctgcgtggcg tcctgcccgc
     4921 cccagacgaa catcttcgtc ccggtccaga ccgagcagtg ccagaagcgc cccgccggcg
     4981 cgttcgtcag cgacgtcggc gtccagacct cgcggtccat cgggacgatc tcggtgaagc
     5041 cctggccgat gagggtcgtg tcgcccggct ttccgagaac cgccgctccg gacggcatca
     5101 ccgcggcgat gctcagcgtc tgcgccgacg gggtgatcgt cacgttgctg ccggccgcca
     5161 gcgtcaggtc gcctgcgagc gtgttgacgc tggtgacgct cggaacggtc gcgtagtagc
     5221 cgttcgtgtc gaggatgacg tcgcctcccg agacgccgag caccaccgag atgccccccg
     5281 tcacgctcag ggggacgacg gcggcgttca cgatgatgtc gttgccgagg tagttcagcg
     5341 tcgacacggg cggaaacgcg ccgccttccg ggtagagcgt gaggaatccc ggcccggtgg
     5401 gcttgacgac cgtcgcgttc agcgagatcg cctgcgcatt cgcaggcacg ttgcagacgc
     5461 ccgcgagcgt gtagctgcgg accgtggcgg gtggcagcca gcctcctccg agcggcgccg
     5521 ggaagaccgg cgctcgcgtg tccacgaggc ggcacgggaa gagcgagatg aacggcagcg
     5581 cgttcgggcc gcccgcgagg ggctggcgcc cggcgagggc gtcgcgattc gccggctggg
     5641 atgcggctcc gccgggaggc atccagtagg gcggcgccgc ccagttgctg atttcatcac
     5701 cgagggcgag cggcgtcagc agggtgatga cgagcaccac cgctgcacga caggcacacg
     5761 ccgagagacg catagatcct cctccatgcg accctccggg gagaagctag tgcctcgagg
     5821 ccgcgcccgc caccacctcc tccggcccgt cgctatcatg ccggcccatg gaccagcgca
     5881 ggaccccgct cctcaagtct tttccggcgg tcttctgggt cgccaacgtc atggagctgt
     5941 tcgagagagc cgcctactac ggactgaact ccgtcctcgc ggtctacctc tcgaacccgg
     6001 cggcaaaggg cggcctcggg ttcccggagc agtcggtcgg gttcctccag agcctgatct
     6061 acgcctgcaa ctacgtcgtg cccatcgcgg gcggcgcgct ggccgaccgg tacggctacc
     6121 ggcggatgct tctcttcgcg ttctcgatgc tcgcgaccgg gtacttcgcg gcgggcaacg
     6181 tcagctcgta cggcgtcgtc ttcgcggcgc tcctcctcat ggcgacgggc ggcggcctct
     6241 tcaagccgat catctccggc acgatcgcgc gctcgacgaa cgacgagaac tccggtttcg
     6301 gcttcggggt ctactactgg atgatcaacg tcggggcgtt cctcgccccg ctcgtcgtca
     6361 gcgtcctgaa ggggttctcc tggcgctggg tcttcatggc gtcctcgggc tactgcgcgc
     6421 tcatgctcct gccggcgctc ttcgccttca aggagccgcc gcggcccgag aacacgaaga
     6481 gcttcggcga cgtcgtccgc ggtgcggcgc tcgtcctcgg ggacgcccgg ttcatgctca
     6541 tgatcgtcgt ctactcgggc ttctggattc tctatttcca gaacttcggg tcggtcctct
     6601 ggtacctgcg cgacttcgtc gacgggacgc cggtggaccg gctcttcgcc tcgttcggca
     6661 tcccgctgaa gttcgactcc gagcacgtga cggtcatcaa cgcgggcacg atcatcctcc
     6721 tgcagatcgt cgtcagccgg gtcgtcaaga acgtccgggc gctcccgacc atggtctcgg
     6781 ggatcgcctt cggcgccctc ggcttcctct gcctcgccct gtcgcggaac gtctgggcct
     6841 tcatcctcgg cctcgccgtc ttctcgatcg gcgagatgac ggcgcacccg aagtactaca
     6901 gctacgtcgg gctcgtcgcg ccccaggaca agaagg
//
LOCUS       DAOMFY010000211         6923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3556_length_6923_cov_6.630897, whole genome shotgun sequence.
ACCESSION   DAOMFY010000211 DAOMFY010000000
VERSION     DAOMFY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3556_length_6923_cov_6.630897"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLB01_14625"
     CDS             complement(<1..367)
                     /locus_tag="PLB01_14625"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HQQ78587.1"
                     /translation="MLALAVVAFRRSSADLFSRLSSADPWLVALGFVLNVLAFAPRAL
                     RLNLLLPRDEEVPFGRAWSVSGATTFLLQVMPFRTGEVGTWAALRSVLGATWARSAAV
                     FALVKLLDTATLLLAGVAGA"
     gene            complement(513..1457)
                     /locus_tag="PLB01_14630"
     CDS             complement(513..1457)
                     /locus_tag="PLB01_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ78588.1"
                     /translation="MKLVIQIPAWDEAEQIAAALAELPKTIEGFDSVETLVVDDGSTD
                     GTAEAARRAGATHVFRLPVHRGLAFAWRAGLDAALRLGADVIVSTDADRQYAASDVAA
                     LVAPILKGEAEIVVGDRGVATKPDFSPGKRMTQRFGSWVVRRASGTDVPDATSGFRAL
                     TREAAMQLNVFSRMTYTIETLIQAGNKEMAVVSVPVRTNAPVRPSRLIRRTSNYVLVQ
                     GANILRITALYKPLPVFFGFAALLTIAGLALVGRYAWFVATAQNPAGHIQSLLLAAVL
                     FVAAVVCFLSGLLADLISINRALLEELVVEKRKRNTEE"
     gene            1521..1781
                     /locus_tag="PLB01_14635"
     CDS             1521..1781
                     /locus_tag="PLB01_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQQ78589.1"
                     /translation="MGFPDEEEKDPDFSDVKSGSSSTAGAPAGEAVTRTYTVVAGDSL
                     SKIARKIYGDGNRWKEIWEANKDTVKNPDLIHPGQQLKIPGA"
     gene            1799..2263
                     /locus_tag="PLB01_14640"
     CDS             1799..2263
                     /locus_tag="PLB01_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78590.1"
                     /translation="MRNRPALPVLVLAAAVSLAACGKKEPPPPPPTAVPAPTAIPTPV
                     PFKVVSVDLGKTLGDDKKVKDAATTFGPKDTIYAVVSTEGVAPKVALKARWTYGAKGT
                     LVNEEGRDISSTGPAATEFHIAKPSGWPVGKYKVEVSADGAVAATKDFEVKK"
     gene            2274..2957
                     /locus_tag="PLB01_14645"
     CDS             2274..2957
                     /locus_tag="PLB01_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQQ78591.1"
                     /translation="MKSLSRVPWLVLALAAVPAGAQDGPPKEERAFRPPDLVELVTLD
                     PTIRLDVRYATKNNEFGRPFYKEARAFLQRPAAEALARVNRALRAKGYGLLVFDGYRP
                     WSVTKQFWDLTPADKKVFVANPKNGSRHNRGGAVDLTLYDLKTGLEADMGGRYDEMTE
                     RSYVTYDKGPKEALARRELLADEMGKEGFFVYAFEWWHFDWKDFREYPVLDVPFAAIG
                     ARLPEAPAR"
     gene            2962..3489
                     /locus_tag="PLB01_14650"
     CDS             2962..3489
                     /locus_tag="PLB01_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HQQ78592.1"
                     /translation="MASLRHRFLMRFVNLYPPLLGAGIRVSYPKDDPYSIEVRMKLHW
                     WNRNLFGTHFGGSLYAMCDPFFVFIVMKNLGPGYLVWDKAVQIEFLRPGRGRVTGRYH
                     VPPEEIARVKALADAGEKVEPVYVGEILADDGTLVARVTKTLWVRKKAPSPAPGQSSA
                     AAPEKTAPPRAAQKP"
     gene            complement(3429..6137)
                     /locus_tag="PLB01_14655"
     CDS             complement(3429..6137)
                     /locus_tag="PLB01_14655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ78593.1"
                     /translation="MTRCPQCDTEVPGSARFCPACGQAVSGSLSHLPTEMGSAPKPVT
                     AKPPSSVRRLVSSGSLDLGAFPPGAMLGERYRIVGLLGKGGMGEVYRADDLKLGQTVA
                     LKFLPKAVSTDESLLARFHAEVRLARQVSHPNVCRIYDIGEIEGRHFLSMEYVDGEDL
                     ASLLKRIGRLPVDKAVEIARQLCAGLHAAHEKGVLHRDLKPANVMLDGRGRVRITDFG
                     LAVAAEEAASLGEISGTPAYMAPEQLAGKPASVRSDLYALGLVLYEIATGRRAFDAPT
                     LAEMRRKHEQETPTAPSNVLAGFDPAVERVILRCLEKDPRARPSSAVQVAGALPGGDP
                     LAAALAAGETPSPEMVAAAGEEGTLAAGPAWALLGGVVVLLAAVLLISGLSRDIGLAP
                     PEKSRDSLTDRAREVAVRLGWKDTPADSATAFSRDYPFLLWMAKNGPPGWARQLATSW
                     WTPASFRYRQSPRPLVPVDFRGFVHSDDPPMTVSGMVTVVLDTRGRLRRFHAVPGQIR
                     NVSAPREEPPWSALFTEAGLEIRSFTPSDAAWVPPVAFDTLAGWTGTLPGKPEAVLKV
                     VAAVHEGRPVYFELIAPWSEAAQVTTRPVSLRAKVFDVSFSLLTLAFTVAGLYFARRN
                     LRLGRGDKAGAIRVTLVVFALILAGHLLVRHMALAPGAFDRAFWESLGDSLPAGLLVG
                     VVYLALEPYFRRRYPELLVSWTRLISGKVRDPLVGRDHLWGVLAGLFVCLVVSVENLP
                     PALFAAPGQTPMPVGNLTLSGLPGIAAHVFFAAADGLVRVFFIAASLFLGRLVFRKPA
                     LAAALPWLILFVAFAGRENPLFEMPGGALCATVFVVLLFRAGLLGLAVGMGVLQLLSN
                     APITPDLSRWFAGYGLFCLAIVLAIAVYGFWAARGGAVFSGAAADD"
     gene            complement(6156..6761)
                     /locus_tag="PLB01_14660"
     CDS             complement(6156..6761)
                     /locus_tag="PLB01_14660"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HQQ78594.1"
                     /translation="MKSSAPPPAAGPRGRPRDPALADRILMAALAQMAEHGYADMSVD
                     AVAAAAGVTKPTLYLRYGSKADLATAALSAFRAAEPPAATGRTRADLVSYLANFRRSL
                     LRPNGLAMIGSVLLEEHRTPDLIARFRERVVAPRRAGLKAILERGVAKRELRRSADLD
                     AAVNLLIGSFYARYLTGEGVPEDWPKRVVAAVWPGLRRAER"
     gene            6813..>6923
                     /locus_tag="PLB01_14665"
     CDS             6813..>6923
                     /locus_tag="PLB01_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQQ78595.1"
                     /translation="MKIAILGAGNVGGALGTGWARKGHTVVFGTRDAGADG"
BASE COUNT         1180 a         2280 c         2589 g          874 t
ORIGIN      
        1 ccgcgcccgc gacgcccgcg aggaggagcg tcgccgtgtc gaggagcttc acgagcgcga
       61 agaccgcggc cgaacgcgcc cacgtcgcac cgaggaccga gcgcagcgcg gcccacgtcc
      121 cgacctcacc ggtccggaac ggcatgacct gcaggaggaa cgtcgtcgcg cccgagaccg
      181 accacgcgcg gccgaacggc acctcctcgt cgcgcggaag cagcaggttc agccggagag
      241 cgcggggcgc gaaggcgagg acgttgagga cgaaccccag cgcgacgagc cacgggtcgg
      301 cggaggagag gcgggagaag aggtctgctg aagagcggcg gaatgcgacg acggccagcg
      361 cgagcaagcc gaagacgaag agaacgccga agattctctc agaaagggta agaggggcgc
      421 ggcgcggggg gcggccgccc gcttcttgcg gagccacatc cgcggcgcgc ggccgtttac
      481 tcaccttcta agaaatccga aatccgaagt cttcactctt ctgtattcct ctttctcttt
      541 tccaccacga gctcctcgag gagggcgcgg ttgatggaga tgagatccgc gaggaggccc
      601 gagaggaagc agacgacggc cgcgacgaag aggacggccg cgaggaggag ggactggatg
      661 tggcccgccg ggttctgcgc cgtcgcgacg aaccacgcgt accgcccgac gagcgcgagg
      721 cctgcgatcg tcaggagcgc cgcgaatccg aagaagaccg gcagcggctt gtagagcgcc
      781 gtgatccgga ggatgttcgc gccctgcacg aggacgtagt tcgaggtgcg gcggatgagc
      841 cgggacggcc gcacgggggc gttcgtccgg acgggcacgg agacgacggc catttccttg
      901 ttgcccgcct ggatgagcgt ctcgatcgtg tacgtcatcc gggaaaagac gttgagctgc
      961 atcgcggcct cccgcgtgag agcgcggaag ccggacgtcg cgtcggggac gtccgtgccg
     1021 gaggcccgcc ggacgaccca tgacccgaac cgctgggtca tgcgcttgcc gggggagaaa
     1081 tcgggcttcg tcgcgacgcc gcggtcgccc accacgatct cggcctcgcc cttcaggatc
     1141 ggggcgacga gcgccgcgac gtccgaggcg gcgtactgcc ggtccgcgtc cgtcgagacg
     1201 atcacgtccg cgccgaggcg cagcgcggcg tcgaggccgg cccgccacgc gaacgcaagg
     1261 ccgcggtgga cggggagccg gaagacgtgg gtcgccccgg cccgccgggc ggcctccgcg
     1321 gtgccgtccg tcgatccgtc gtcgacgacg agcgtctcga cggagtcgaa gccctcgatc
     1381 gtcttcggca gctccgcaag ggcggccgca atctgctccg cctcgtccca ggcgggaatc
     1441 tggatgacga gcttcatggt gcctgcagaa ggttagcagg ggggcgtgat atgacgttcg
     1501 ctgcaccagg aggtgtcgac atgggctttc ccgacgaaga agagaaggac cccgatttct
     1561 cggacgtgaa gagcggcagc tcctcgacgg ccggcgcccc cgccggcgag gccgtgacgc
     1621 ggacctacac cgtcgtcgcc ggcgacagcc tgtcgaagat cgccaggaag atctacggcg
     1681 acgggaaccg ctggaaggag atctgggaag cgaacaagga cacggtcaag aaccccgatc
     1741 tcatccaccc cggccagcag ctgaagattc ccggcgcctg agtacgaagg agaagacgat
     1801 gagaaaccgc cccgccctgc ccgtgctcgt cctcgcggcc gccgtctcgc tcgcggcctg
     1861 cggaaagaag gaaccgccgc cgccgccccc gaccgcggtg ccggccccga ccgcgatccc
     1921 gacgcccgtg ccgttcaagg tcgtgtccgt cgacctcggc aagaccctcg gcgacgacaa
     1981 gaaggtcaag gacgccgcga cgaccttcgg gccgaaggac acgatctatg ccgtcgtgtc
     2041 gacggagggc gtcgcgccca aggtcgccct caaggcgcgc tggacctacg gcgccaaggg
     2101 cacgctcgtg aacgaggagg gccgcgacat ctcctcgacc ggtccggcgg ccaccgagtt
     2161 ccacatcgcg aagccctcgg gctggcccgt cgggaagtac aaggtggaag tctcggccga
     2221 cggcgccgtc gccgcgacga aggacttcga ggtcaagaag tagcttcggc ccggtgaaga
     2281 gtctgtcccg cgtcccgtgg ctcgtcctcg cgctcgccgc cgtgccggcg ggcgcgcagg
     2341 acggcccgcc taaagaagag cgcgcgttcc gcccgcccga cctcgtcgag ctcgtgacgc
     2401 tcgaccccac gatccgcctc gacgtgcgct acgcgaccaa gaacaacgaa ttcggccggc
     2461 ccttttacaa ggaggcccgc gccttcctcc agcgtccggc cgcggaggcc ctcgcgcgcg
     2521 tgaatcgcgc actgagggcg aagggctacg gccttctcgt cttcgacggc taccggccgt
     2581 ggtccgtgac gaagcagttc tgggacctga cgccggccga caagaaggtg ttcgtcgcga
     2641 acccgaagaa cggctcgcgg cacaaccggg gcggcgccgt cgacctcacg ctgtacgacc
     2701 tcaagaccgg gctcgaggcc gacatgggcg ggcggtacga cgagatgacg gagcgctcgt
     2761 acgtcacgta cgacaaaggg ccgaaggagg cgctcgcacg gagggagctg ctcgccgacg
     2821 agatggggaa ggaaggcttc ttcgtctacg cgttcgaatg gtggcacttc gactggaagg
     2881 acttccggga gtaccccgtc ctcgacgtcc cgttcgccgc gatcggggcg cggctccccg
     2941 aggcccccgc gcgctgagac gatggcttcg ctccggcacc gattcctgat gaggttcgtg
     3001 aacctgtacc cgccgctcct cggggccggg atacgggtca gctacccgaa ggacgacccg
     3061 tactcgatcg aagtgcggat gaagctccac tggtggaaca ggaatctctt cgggacgcac
     3121 ttcggagggt cgctctacgc gatgtgcgac ccgttcttcg tgttcatcgt gatgaagaac
     3181 ctcggtcccg ggtacctcgt ctgggacaag gcggtgcaga tcgagttcct ccggccgggc
     3241 cgcgggcgcg tgaccgggcg ttaccacgtc ccgcccgagg agatcgcgcg cgtgaaggcg
     3301 ctcgcggacg cgggggagaa ggtcgagccg gtctacgtcg gcgagatcct cgcggacgac
     3361 gggacgctcg tggcgcgcgt gacgaagacg ctctgggtga ggaagaaagc gccctcgccg
     3421 gcgcccggtc agtcgtcggc cgcggcgccg gaaaagacgg ctccgccgcg tgcggcccag
     3481 aagccgtaga ccgcgatcgc gaggacgatc gcgaggcaga agagcccgta gccggcgaac
     3541 cagcgggaga ggtcgggcgt gatcggcgcg ttcgagagga gctggagaac gcccattccc
     3601 accgcgaggc cgaggaggcc ggcgcggaag agaaggacga cgaagaccgt cgcgcagagg
     3661 gctccccccg gcatctcgaa gagaggattc tcgcggccgg cgaacgccac gaagaggatg
     3721 agccacggca gggcggcggc gagcgcgggc ttgcggaaga cgagccgtcc gaggaagagc
     3781 gatgcggcga tgaagaagac gcggacgaga ccgtccgcgg cggcgaagaa gacgtgggcc
     3841 gcgatgccgg ggaggccgga gagggtgagg ttgcccaccg gcatcggcgt ctggccgggc
     3901 gccgcgaaga gcgcgggcgg gaggttctcg acgctcacga cgaggcagac gaaaaggcct
     3961 gcgagaacgc cccacagatg gtcccggccg acgagagggt cgcgcacctt gccggagatg
     4021 agccgggtcc acgagacgag gagctcgggg taacgccgcc ggaagtacgg ctccagggcg
     4081 agatagacga cacccacgag gagtcccgca ggaagactgt cgccgaggct ctcccagaac
     4141 gcacgatcga aggccccggg cgcgagggcc atgtggcgca cgaggagatg gccggccagg
     4201 atgagcgcga agacgacgag ggtcacgcgg atcgcgcccg ccttgtcgcc gcgtccgagc
     4261 cggaggttgc gccgcgcgaa gtagagcccc gcgacggtga acgcgagagt gagaagagag
     4321 aaggagacgt cgaagacctt cgcgcggagc gagacgggtc gggtcgtgac ctgagcggcc
     4381 tcgctccagg gcgcgatcag ctcgaagtag acggggcggc cctcgtggac cgcggcgacg
     4441 accttcaaaa ccgcttccgg cttcccgggg agggttcccg tccagcccgc cagcgtgtcg
     4501 aaagcgacgg gcggcaccca tgcggcgtcg gagggcgtga aggacctgat ctcgaggccg
     4561 gcctcggtga agagcgcgct ccacgggggc tcctcgcggg gagcggagac gttccgaatc
     4621 tggcccggaa cggcgtggaa gcggcgcagc cgtccgcgcg tgtcgaggac gaccgtgacc
     4681 atcccggaga cggtcatcgg ggggtcgtcg gagtggacaa acccccggaa atcgacgggc
     4741 acgaggggcc gagggctctg gcggtaccgg aacgaggccg gcgtccacca ggaggtggcg
     4801 agctggcgag cccagccggg cgggccgttc ttggccatcc acaggaggaa cgggtagtcg
     4861 cggctgaaag ccgtcgcgga gtcggcgggc gtgtccttcc agccgagtcg caccgccacc
     4921 tcacgggcgc ggtccgtgag cgaatcgcgg gacttctcgg gtggcgcgag gccgatgtcc
     4981 cgcgagaggc ctgaaatgag gagcaccgcg gcgagaagta cgaccacgcc gccgaggagt
     5041 gcccaggcag gtcccgcagc gagcgtccct tcctctccgg ccgccgcgac catctcgggg
     5101 gagggcgtct cgccggccgc gagggccgcg gcgagcgggt cgccccccgg gagcgccccc
     5161 gcgacctgca cggccgacga gggccgcgcg cgcgggtcct tctcgaggca gcgcaggatc
     5221 acgcgctcga cggcgggatc gaaccccgcg aggacgttcg agggcgccgt cggcgtctcc
     5281 tgctcgtgct tgcggcgcat ctcggccagc gtcggcgcgt cgaacgcgcg cctgcccgtc
     5341 gcgatctcgt agaggacgag gccgagcgcg tagagatcgc tccggaccga ggcgggcttg
     5401 cccgcgagtt gttccggggc catgtaggcg ggcgtgcccg agatctcccc cagcgaggcc
     5461 gcttcttcgg cggccaccgc gaggccgaag tccgtgatcc ggacgcgccc gcgcccgtcg
     5521 agcatgacgt tcgcgggctt gaggtcgcgg tggaggacgc ccttctcgtg cgcggcgtgc
     5581 aggcccgcgc agagctggcg ggcgatctcg accgccttgt cgacggggag gcgcccgatc
     5641 cgcttcagga gcgacgcgag gtcctcgccg tccacgtact ccatggagag gaagtggcgg
     5701 ccttcgatct cgccgatgtc gtaaatgcgg cagacattcg ggtgcgagac ctggcgcgcg
     5761 aggcgcacct cggcgtggaa gcgcgcgagg agactctcgt ccgtcgagac cgccttgggc
     5821 aggaacttga gggcgacggt ctggccgagc ttgaggtcgt ccgcgcggta gacctcgccc
     5881 atcccgccct tcccgagcag cccgacgatg cgataacgct cgccgagcat cgcgccgggg
     5941 gggaacgcgc cgaggtcgag cgagcccgac gaaacgagcc gccggaccga cgaaggcggc
     6001 ttcgccgtca cgggctttgg agccgagccc atctcggtgg ggaggtgcga gagcgaaccc
     6061 gacacggcct ggccgcacgc cgggcagaaa cgggccgagc ccggcacctc ggtgtcgcac
     6121 tgagggcagc gtgtcacgtg tgtcgagatt ctatctcagc gttcggcgcg tctcaggccg
     6181 ggccagaccg cagcgacgac gcgtttcggc cagtcttccg ggacaccttc acccgtgagg
     6241 tagcgcgcgt agaaggagcc gatgaggagg ttgacggcgg cgtcgaggtc ggcgctgcgg
     6301 cgcagctccc gcttcgccac gccccgctcg aggatcgcct tgaggccggc gcggcggggc
     6361 gcgacgacgc gctcgcggaa ccgggcgatc aggtcgggcg tgcggtgctc ttccaggagg
     6421 acgcttccga tcatcgcgag gccgttcggt cgaagaagag aacgccggaa gttcgcaagg
     6481 tagctgacga ggtccgcccg ggtacgcccg gttgcggccg ggggctcggc ggcccggaac
     6541 gcggagagcg ccgccgtcgc gaggtcggcc ttgctcccgt agcgcaggta gagcgtcggc
     6601 ttcgtgacgc ccgcggccgc ggccaccgcg tccacggaca tgtccgcgta gccgtgctcc
     6661 gccatctgcg ccagcgcggc catcaggatc cggtccgcga gcgccgggtc ccgggggcgg
     6721 ccgcgcgggc cggcggcggg cgggggagcg cttgacttca tttaccagca agtatagtaa
     6781 tccggcccgc cgttcgcggc gtagggggag ccatgaagat cgcgatcctc ggagcgggaa
     6841 acgtgggcgg ggcgctcggg acgggctggg cccggaaagg ccacacggtc gtcttcggga
     6901 cgcgcgacgc cggggccgac ggc
//
LOCUS       DAOMFY010000212         6879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3601_length_6879_cov_5.859174, whole genome shotgun sequence.
ACCESSION   DAOMFY010000212 DAOMFY010000000
VERSION     DAOMFY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3601_length_6879_cov_5.859174"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLB01_14670"
     CDS             <1..807
                     /locus_tag="PLB01_14670"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQQ78596.1"
                     /translation="IVVTGGFVGATPDGVTTTLGRGGSDYSAALFGAALRDAGRDVGA
                     IEIWTDVDGILTADPRVVPAARLVPEVSAAEAAELAFFGAKVLHPATIRPAVARGIPV
                     AVKNSFRPDAPGTVVLKDAPGAGVRALAMRTGVAAVFVGNPRMLLAHGYAARVFSVFE
                     KHAVPVDVIATSEVSISITVDAKAPLGAVVKDLSAFAEVSVLPDLAVVSVVGKALRTT
                     AGIARRVFQALGDVNVVLISQGASDTNLTFVVAAGDAPEALRRLHKEFFE"
     gene            804..1550
                     /locus_tag="PLB01_14675"
     CDS             804..1550
                     /locus_tag="PLB01_14675"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQQ78597.1"
                     /translation="MRALLVGYGKMGRAIEAVLVTRGHGVTGRFGGREEGDFLEGNGA
                     SGGVGAEACDVAFEFTAPEAAPRLVPRLLSKKIPVLSGTTGWDTGEAVRLATELSVPF
                     LHSANFSLGVAAMRRGAAVVAAALAPFREFHPGLVERHHSAKRDAPSGTARALAADVT
                     HASGRRDVPIASLRQGGVPGEHTLYFEGEDETVEIVHRARSRAIFAQGAVRSAEWMLA
                     SGRTGPLTFDDFFAAASRTDASRENGRRTS"
     gene            1547..2497
                     /gene="dapA"
                     /locus_tag="PLB01_14680"
     CDS             1547..2497
                     /gene="dapA"
                     /locus_tag="PLB01_14680"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQQ78598.1"
                     /translation="MSSSPSKNLPSFSGVYTALVTPFRDDGALDEPGLRRLVRRQITG
                     GVAGLVPCGTTGEAVTLDVDEHERVVAITVEEARAAPRPVQVIAGCGSNDTRKAQRLA
                     ARCRSAGADALLVVTPYYNKPTPRGLVAHFSAVADAGQIPVVAYNVPGRTGLNMLPET
                     VLELARDPRIAGVKEASGSLDQSCEILRARPAGFAVLSGEDSLALPIVACGGDGVIAV
                     VSNEAPALLVELVAAALAGDRPRAAALQARLLPLMKANFRESNPIPVKWALERLGLVG
                     GALRLPLVPLSPAHFGPMEAALKAAGLLDEAGRPLEAVSA"
     gene            2494..3261
                     /locus_tag="PLB01_14685"
     CDS             2494..3261
                     /locus_tag="PLB01_14685"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQQ78599.1"
                     /translation="MTREYVFRLLDALERGEVRAAAPGPDGFWAADASVKDGILAAFR
                     LGIEEPSSAGPLHFRDRDTLPPRSPLPEGVRVVPGGTALRRGAFLAKGVVVMPPAYVN
                     VGAYVDEGSMIDSHALVGSCAQVGKRVHLSAAAQVGGVLEPAGAVPVVLEDEVFVGGG
                     AGVYEGVCVRRRAVLAAGVILTASSVLFDLVHEKEYRSTPERPLVVPEGAVVVMGARP
                     APGSWGKCHGLSLAAPVIVKYRDAKTDARTVLESALR"
     gene            3298..4572
                     /locus_tag="PLB01_14690"
     CDS             3298..4572
                     /locus_tag="PLB01_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78600.1"
                     /translation="MNRASGDGVLLRAARRFGTPLYLYDFDVIEARVRALRSALGPRF
                     TLAYAVKANPSLGILRFLTRFGLSSDVASRGELAAVRRAGWSPSRILSTGPAKSDADL
                     EALVKARVSIIHAEGEWELEQLERIGRRLKRRVSVGLRLNPPWGIAEKKVIIGGPGAK
                     KFGVDIRTAGKILRAKGKFPHLDICGFQVFNASNVLNARLLVENTRNVLELALSLSRR
                     FLVPLRSIDFGGGFGVPYAEGEKPLDLGFLGRGLMRVAREARASGLLDSTRLVFEPGR
                     FLVAEAGTYVTKVLGTKKSLGVDYVLVDGGVHHFLRPVLLDSPHRVRLIRQKASGPPA
                     PSSPSKKSLVLSGPLCTSLDVLHPSARLPMPRRGDLLAFENAGAYGYTESMPLFLSHE
                     WPAEAGVRKGRVALLRRPPRVAELQVRQKTPF"
     gene            4619..5608
                     /locus_tag="PLB01_14695"
     CDS             4619..5608
                     /locus_tag="PLB01_14695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQQ78601.1"
                     /translation="MDVRSLGTHGPSVSAIGLGCMGMSEFYGTRNDDESILTIHAALD
                     AGVDFFDTADMYGPYTNETLVGKALADRPGRAVLSTKFGIVRDAADPTKRGVCGKPDY
                     VRSSCEGSLKRLGVETIDLYTLHRVDPATPIEDTVGAMAGLVKEGKVRWIGLSEASAD
                     TLRRASRVHPIAALQSEYSLFSRDVEAAVLPACRELGIGFVAYSPLARGFLSGKYRSL
                     ADFPEGDYRRLAPRFQGGNFAKNLALVAKVADLARRRKATPAQVALAWVLSRGADVVP
                     IVGTKTRERLEENLNATDLVLRAADLAELEAAFPVGAAAGERYPAAAMATVGR"
     gene            5654..>6879
                     /locus_tag="PLB01_14700"
     CDS             5654..>6879
                     /locus_tag="PLB01_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQQ78602.1"
                     /translation="MSVKVPETAALPLSPEQERIRAAKEAWRLKAAAAFAKTPAWRKD
                     FTTVSSAEVNTLATPDDLGGFDYARDLGWPGEFPYTRGVQPTMYRGKPWTIRQFAGFG
                     TARQTNERFKQLLAHGQTGLSTAFHLPTLYGYDSDHAMAAGEVGKCGVAVDTLRDFEA
                     LFDGIDLGAVTTSMTINSTAPIALAMYLAVAEKHGTPWAKVGGTLQNDILKEYIAQKE
                     YIFPPRPSMRLVTDQFAFCAKHVPRWNTVSISGYHIREAGSTALQELAFTLRDGMEYV
                     KYGIDAGLDVDDFAPRLSFFFNAHNDFFEEIAKYRAARRIWAKVMRDRYGAKKAESWK
                     MRFHSQTAGVTLTAQQPYNNVARVALQSLAAVLGGTNSLHANALDETLALPTEFAAKI
                     AVRTQQVILHETGVVNT"
BASE COUNT          981 a         2426 c         2410 g         1062 t
ORIGIN      
        1 atcgtcgtca cggggggatt cgtcggcgcc acgccggacg gcgtcacgac gacgctcggc
       61 cggggtggct cggattactc ggcggcgctc ttcggcgcgg cgctgcggga cgcgggccgg
      121 gacgtcgggg ccatcgagat ctggacggac gtggacggca ttctcacggc cgacccgcgc
      181 gtcgtccccg ccgcgcggct cgtgccggag gtcagcgctg ccgaggccgc cgagctggcg
      241 ttcttcggcg cgaaggtcct gcacccggcc acgatccgcc cggccgtcgc gcgcgggatc
      301 cctgtcgcgg tgaagaactc gttccggccg gacgcgccgg gcaccgtggt cctgaaggac
      361 gcgcccggcg ccggcgtccg cgcgctcgcg atgcgcacgg gcgtcgcggc cgtgttcgtc
      421 gggaacccgc gcatgctcct cgcgcacggc tacgcggccc gggtgttctc ggtcttcgag
      481 aagcacgccg tgccggtgga cgtcatcgcg acgagcgagg tctcgatctc gatcacggtg
      541 gacgcgaagg cgccgctagg ggccgtcgtg aaggacctct ccgcgttcgc cgaggtgtcg
      601 gtcctccccg acctcgccgt cgtgtccgtc gtcggcaagg ccctccgcac gacggccggc
      661 atcgcgcggc gcgtgttcca ggcgctcgga gacgtcaacg tcgttctcat ctcgcagggc
      721 gcgtcggaca cgaacctgac gttcgtcgtc gccgcgggcg acgcgccgga ggcgctccgc
      781 cgcctgcaca aggagttctt cgaatgagag cgctgctcgt cggatacggg aagatgggaa
      841 gggcgatcga ggcggtgctg gtcactcgcg ggcacggggt cacggggaga ttcggaggaa
      901 gagaagaggg ggatttcttg gaaggtaatg gggcatcggg gggcgtcggt gcggaagcct
      961 gcgacgtcgc gttcgagttc accgccccgg aggccgcacc gcgcctggtg cctcgcctcc
     1021 tttcgaagaa aatccccgtt ctctccggga ccaccggctg ggacacgggt gaggcggtcc
     1081 ggctcgcgac ggagctctcg gtgccgttcc tccactccgc gaacttctcc ctcggcgtcg
     1141 ccgcgatgcg ccgcggcgcg gcggtggtcg cggcggcgct cgcaccgttc cgcgagttcc
     1201 acccgggcct cgtcgagcgg caccactcgg cgaagaggga cgcgccctcg ggcacggcaa
     1261 gggcgctcgc ggccgacgtc acccacgcgt ctggccgaag ggacgtgccg atcgcgtcgc
     1321 tgcggcaggg aggcgtcccg ggagagcaca cgctctactt cgaaggcgag gacgagacgg
     1381 tcgagatcgt ccaccgcgcg cgttcgcgcg cgatcttcgc gcagggcgcc gtccgctcgg
     1441 cggagtggat gctcgcttcc ggccggacag gccccctgac cttcgacgat ttctttgcag
     1501 ccgcgtcgcg caccgacgcg tcacgcgaaa acggaaggcg gacctcatga gctcttcacc
     1561 ttcgaagaat cttccgtctt tctcgggcgt gtacacggcc ctcgtcaccc cgttccggga
     1621 cgacggcgcg ctcgacgagc cgggcctgcg acgcctcgtg cgccggcaga tcacgggcgg
     1681 cgtcgcgggc ctcgtgcctt gcggaaccac gggcgaggcc gtgacgctcg acgtggacga
     1741 gcacgagcgc gtcgtcgcga tcacggtcga ggaggcgcgc gcggcgccgc gccccgttca
     1801 ggtgatcgcg ggatgcggct cgaacgacac gcggaaggcg cagcgcctcg cggctcgctg
     1861 ccggtcggcg ggagccgacg cgctgctcgt cgtgacgccc tactacaaca agccgacgcc
     1921 gcgcggactc gtcgcgcact tctccgcggt ggcggacgcg ggccagattc cggtcgtcgc
     1981 gtacaacgtg ccgggccgca cgggcctcaa catgcttccc gagaccgtgc tcgagctcgc
     2041 gagagacccg cgcatcgcgg gcgtgaaaga ggcgtccggc agcctcgacc agtcctgcga
     2101 gatcctgcgg gcgcggcccg ccgggttcgc ggtcctctcg ggcgaggatt cgctcgccct
     2161 cccgatcgtc gcgtgcggcg gcgacggcgt gatcgccgtg gtctcgaacg aggcgcccgc
     2221 cctcctcgtc gagctggtcg ccgccgcgct cgcaggcgac cggccgcgcg ccgcggccct
     2281 gcaggcacgg ctcctgcctc tcatgaaggc gaacttccgc gagtcgaatc cgatccccgt
     2341 caagtgggcg ctggagcggc tggggctcgt gggcggcgcc ctccggctgc cgctcgtgcc
     2401 gctgtcgccg gcgcatttcg gaccgatgga agcggcgctg aaggccgccg gcctgttgga
     2461 cgaggccgga cggcccctcg aggcggtgtc cgcgtgacgc gcgagtacgt gtttcgcctc
     2521 ctcgacgctc tcgagcgcgg ggaggttcgg gccgccgcgc cggggccgga cgggttctgg
     2581 gccgccgacg cctcggtgaa ggacggcatc ctcgcggcgt tccggctcgg catcgaggag
     2641 ccgtcctccg ccgggccgct ccacttccgc gaccgcgaca cgctcccgcc gcgctcgccg
     2701 cttcccgagg gcgtgcgcgt cgttccgggc gggacggccc tccggcgcgg cgcctttctc
     2761 gcgaagggcg tcgtcgtcat gcccccggcc tacgtcaacg tgggtgcata cgtcgacgag
     2821 ggctcgatga tcgacagcca cgcgctcgtc ggctcgtgcg cgcaggtggg caagcgcgtg
     2881 catctctcgg cggcggcgca ggtcggcggc gtcctcgagc cggcgggagc cgtgccggtc
     2941 gtcctcgagg acgaggtctt cgtgggcggg ggcgcgggcg tctacgaggg cgtgtgcgtg
     3001 cggcggcgcg cggtgctcgc ggcgggcgtg atcctgaccg cgtcgagcgt cctcttcgac
     3061 ctcgtgcacg agaaggaata ccggtcgacg cccgagcgcc ccctcgtcgt gccggaagga
     3121 gccgtcgtgg tcatgggcgc gcgccccgcc ccgggctcgt ggggcaagtg ccacggtctg
     3181 tcgctcgcgg cgccggtgat cgtgaagtac cgcgacgcga agacggacgc acggacggtc
     3241 ctcgagagcg ctctgcgatg aagaagagaa gagaagaaga gaaagatttc ttagaaggtg
     3301 aatagggcat ccggcgatgg cgttctcctt cgggccgcgc ggcggttcgg gacgcctctt
     3361 tatctctacg acttcgacgt gatcgaggcg cgcgtgcgcg cgctgcgttc ggccctcggt
     3421 ccgcgcttca cgctcgcgta cgcggtcaag gccaacccct cgctcgggat tcttcgcttt
     3481 ctgacacgat tcggtctctc ttcggacgtg gcttcgaggg gcgagctcgc cgccgtgcgc
     3541 cgcgccggct ggtcgccatc gagaattctc tcgaccggtc ccgcgaagtc ggacgccgac
     3601 ctcgaagcgc tcgtgaaggc gcgcgtctcg atcatccacg ccgaagggga gtgggagctc
     3661 gagcaactgg agagaatcgg caggcgcctc aaaaggcggg tctccgtcgg cctccggctg
     3721 aatccgccct gggggatcgc ggagaagaag gtcatcatcg gcggccccgg ggcgaagaag
     3781 ttcggcgtcg acatccggac cgccgggaag attcttcggg cgaaagggaa atttccccat
     3841 ctcgacatct gcgggttcca ggtcttcaac gcctcgaacg tcctcaatgc gcgccttctc
     3901 gtcgagaaca cgcgaaacgt cctggaactc gctctttcac tttctaggag gttcttggtc
     3961 cctcttcgtt cgatcgactt cggcgggggt ttcggagtgc cgtacgcgga gggagagaaa
     4021 cccctggatc tcggatttct tggaaggggg ttgatgcggg ttgcgaggga ggcgcgcgcg
     4081 agcgggctgc tcgactccac ccgcctcgtg ttcgagccgg ggcggttcct cgtcgccgag
     4141 gccggaacgt acgtgacgaa ggttctcgga accaagaaga gtctcggcgt cgactacgtc
     4201 ctcgtcgacg gcggcgtcca ccacttcctc cgcccggttc tcctcgacag cccgcaccgc
     4261 gtgcgcctca tccggcaaaa ggcgagcggc ccccccgcgc cctcttcacc ttcaaagaaa
     4321 tctctcgtcc tctctggccc tctctgcacg tctctcgacg tcctgcaccc gtccgcgcgg
     4381 ctgcccatgc cccggcgcgg cgacctcctc gcgttcgaga atgccggcgc gtacgggtac
     4441 acggagtcga tgccgctctt cctgtcgcac gagtggcccg cggaagccgg cgtccggaag
     4501 ggccgtgtcg cgctgcttcg ccgtccgccc cgcgtcgccg agctgcaggt ccgccagaag
     4561 acgccgttct gacggcgcgg gcggcggagg gataatcggc ggccccaggg aggcggccat
     4621 ggacgttcgc tcgctcggca cgcacggtcc cagcgtctcc gcgatcggac tcggctgtat
     4681 ggggatgtcc gagttctacg gcacccgcaa cgacgacgag tcgatcctca cgatccacgc
     4741 ggccctcgac gccggcgtcg atttcttcga cacggccgac atgtacggcc cgtacacgaa
     4801 cgagacgctc gtcggcaagg ccctcgcgga ccggcccggc cgggccgtcc tctcgacgaa
     4861 gttcggcatc gtgcgcgacg cggcggaccc gacgaagcgc ggcgtctgcg ggaagccgga
     4921 ctacgtgcgg tcatcctgcg aggggagcct caagcgcctc ggcgtcgaga cgatcgacct
     4981 ctacaccctt caccgcgtcg accccgcgac gccgatcgag gacacggtcg gcgcgatggc
     5041 gggcctcgtg aaggagggca aggtccgctg gatcggactc tccgaggcgt cggcggacac
     5101 tctccggagg gcgagccggg tccacccgat cgccgcactc cagagcgagt attcgctctt
     5161 ctcgcgcgac gtcgaggcgg ccgtcctgcc cgcgtgccgc gagctcggga tcggattcgt
     5221 cgcctacagt ccgctcgcgc gcgggtttct ctcgggcaag taccgcagcc tcgcggactt
     5281 tccggaaggc gactaccgcc gcctggcgcc gcgctttcag ggcgggaatt tcgcgaagaa
     5341 cctcgcgctc gtcgcgaagg tggccgacct cgcgcgccgc cgcaaggcga cgcctgcgca
     5401 ggtcgcgctc gcctgggtgc tctcgcgcgg cgccgacgtc gtgccgatcg tggggacgaa
     5461 gacccgcgag cggctggagg agaacctcaa cgcgaccgac ctcgtcctca gggcggccga
     5521 tctcgcggaa ctcgaggccg cattccccgt cggggccgcg gccggggagc gctatccggc
     5581 ggccgccatg gccacggtcg gccgctgagt ctccctctcg gtgcgcgggc cccggcccga
     5641 tagaatcccc gacatgagcg tcaaggttcc cgaaaccgcc gcactccccc tgagccccga
     5701 gcaggagcgc atccgcgccg cgaaggaagc gtggcggctg aaggccgcgg ccgcgttcgc
     5761 gaagacgccc gcgtggcgga aggacttcac gaccgtctcg tcggccgagg tgaacactct
     5821 cgcgacgccc gacgacctcg gcggcttcga ctacgcgcgt gacctcggct ggccgggcga
     5881 gttcccgtac acgcgcggcg tccagcccac gatgtaccgg ggcaagccgt ggacgatccg
     5941 gcagttcgcg ggcttcggca cggcgaggca gacgaacgag cgcttcaagc agctcctcgc
     6001 gcacggccag acgggcctct cgacggcgtt ccacctcccg acgctctacg gctacgactc
     6061 cgaccacgcc atggctgcgg gtgaggtcgg gaaatgcggt gtggcggtcg acacgctccg
     6121 ggacttcgag gcgcttttcg acggaatcga cctcggcgcc gtgacgacgt ccatgacgat
     6181 caattccacc gctcccatcg cgctcgcgat gtacctcgcg gtcgccgaga agcacggcac
     6241 gccgtgggcg aaggtcggcg gcactctcca gaacgacatc ctcaaggaat acatcgcgca
     6301 aaaggagtac atcttcccgc cgcgcccctc gatgcggctc gtgacggacc agttcgcgtt
     6361 ctgcgcgaag cacgttccgc gctggaatac cgtctcgatc tccggctacc acatccgcga
     6421 ggccggctcg accgcgctcc aggagctcgc cttcacgctc cgcgacggca tggagtacgt
     6481 gaagtacgga atcgacgccg ggctcgacgt cgacgacttc gcgccgcgtc tctccttctt
     6541 cttcaacgca cacaacgact tcttcgagga gattgcgaag taccgggcgg cccgccggat
     6601 ctgggcgaag gtcatgcgcg accggtacgg cgcgaagaag gccgagagct ggaagatgcg
     6661 cttccactcc cagaccgccg gcgtcactct cacggcgcag cagccgtaca acaacgtcgc
     6721 gcgcgtcgcg ctgcagtccc tcgccgccgt gctcgggggc acgaactccc tccacgcgaa
     6781 cgcgctcgac gagacgctcg cgctcccgac ggagttcgcc gcaaagatcg cggtccggac
     6841 ccagcaggtc atcctccacg aaacgggcgt cgtgaacac
//
LOCUS       DAOMFY010000213         6870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3610_length_6870_cov_7.015701, whole genome shotgun sequence.
ACCESSION   DAOMFY010000213 DAOMFY010000000
VERSION     DAOMFY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3610_length_6870_cov_7.015701"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLB01_14705"
     CDS             complement(<1..615)
                     /locus_tag="PLB01_14705"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQQ78603.1"
                     /translation="MKRIVTIAAGALALAVASVPAFAQTAAAPATSQAASGSKIGVIE
                     VQRIVQESAIGKESLARVQKLQQTKQEELTKRQKDLRDLETKIQDQGKALSEEAMEKL
                     QKEYQGKAVELKRFQDDAQKELEESQRKELGDLEKRIMPVINEVAREQGYQLVFNKFN
                     SGLLFADDKSVDLTEAVITKFNSQSAAPKAAAAKPAAAAAPAPAA"
     gene            complement(694..3300)
                     /gene="bamA"
                     /locus_tag="PLB01_14710"
     CDS             complement(694..3300)
                     /gene="bamA"
                     /locus_tag="PLB01_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQQ78604.1"
                     /translation="MRPCLLSGASAGSAVRSIRALAGAGLFGALLLAGPAGAQAPQPA
                     PAPTPAPDAAFGPKTESPLSGAMGAAPSVRLASEGEKGAAQKLPRLVQIVVKGGKTTA
                     SETVGFYLGLKPGDPYDDELVKRNFSKLWDSGLFEDVTLEKDETPQGVVLTATVVERP
                     TIADLEFRGNKKLTTSQLKDKLKEGKAEIRIGAPVSLRDVAKAKGVLLEGYKAEGFRS
                     AVVEAQLEIVSAAQRRVVFNVDEGDKMKVESINFTGNTVFGAQTLRNAMKKTATAHWW
                     RFFDSKSTFNQASYDEDVESLRKAYQDVGYKDVVVKDAIIDTFVVNPKEKRPDKIKRR
                     ARLTIPIVEGDRWFFGKLNVDGSTVYKPEILEKFFGYRTGKPLSRAILADGMKSIEAI
                     YRSQGYIYVFMNPEFTEKPNHVVDVTVKIAEGDQYRLGRVEFQGNKTTRDKVLRRELQ
                     LYEGDIMDMETFKKSIQKVTQLGYFKVEEDPDFKVNNDKKTVDITIKGTDTNRNEVQF
                     GAGYSQLDGLFGQFQFNTRNFLGRGDTLGLQFQRGNRSNFFDVSFSEPWFLDQRMSVG
                     GAIFNRTLDYSDVAGGTNQSSKGLNLSLGVGLGVWDVVSLFYGFTDTKSRYQVYPPPP
                     PPGGGYTPPAFADYTGKTSSITPGYRFDSRNDPFDPTRGLRYGASVTIAGGALGGDFS
                     FVKSAASVTAYLPATKKSNFAVNFELGMLFPYDSQAQREAGRSEIPIFERYRIGGDRS
                     VRAFKYGAIFPLDDENRAFFDQNGALLGGNRFVVFNLEYVYQLAQPLKLVAFFDAGNT
                     WIEGQTVNLLKMRTAVGLEMRIFLPIFQAPLRFIYGINLQPKVILDQTGKPLANGEES
                     KTDFQFSIGTTF"
     gene            complement(3306..>4964)
                     /locus_tag="PLB01_14715"
     CDS             complement(3306..>4964)
                     /locus_tag="PLB01_14715"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQQ78605.1"
                     /translation="KGILKELKENQDLIVFIDEIHSLIGAGSAEGSLDAANILKPALS
                     RGEISCIGATTLKEYRKYIEKDRSLLRRFQAVTVAPPDEEETLQILEGVRERYEKFHR
                     VRYTPDAIKTAVYQASRYIPDRFFPDKAIDILDEAGAKVKLRKTADTQNLRRLENEIK
                     GIVKEMKKAISEKDFERAVFLREREIEIKEEIERTKTAAQDESAAVDVTRADIEDVIS
                     SWTGIPVTSLQMEEAEKLLHMEETLKKRVIGQDRSIGAISRAIRRSRLGVKSPNRPMG
                     SFIFLGPSGVGKTEVARRLAEFLFDNQKALVRFDMSEYMEKHAVSKLIGSPPGYVGHE
                     EGGQLTERIRRQPYSVILLDEIEKAHPDIANLLLQILEDGQLTDAYGNVVDFKNTLII
                     MTSNVGTKYLVTGSRMGFGEGKNAPEQREVEEMVLKELRREFSPEFINRIDEVIVFNP
                     LGLEQLRGIGRLLLDDVAETLRHRGLKLVASQAAVDWLLKASGHDAHSGARPLRRTIQ
                     RHVEDAVSELLIASREPIECVEVTVEGDELRVQIRAPEPVGTGR"
     assembly_gap    4965..5064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5065..5887)
                     /locus_tag="PLB01_14720"
     CDS             complement(<5065..5887)
                     /locus_tag="PLB01_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HQQ78606.1"
                     /translation="MFEKYNEKARRALFFARYESSKLGSKVIESEHILLGILREGEDV
                     IKEIFARFNVKPEDIRHEIEGDRVFVERVSSTQELPLSEESKKILAYASHEAESMMHP
                     YVGTEHLLIGILRVDPCVAGRILMAHGFNLYGVREETIALIKEREASKQKKELPFLAE
                     YSRDLTALAQTGSFDPLVGRDAEVERIVQILSRRTKNNPILLGEPGVGKTAIVEGLAQ
                     KIVEGGVPLFLAQRKILALDLSLIVAGTKYRGQFEERLKGILKELKENQDLIVFID"
     gene            complement(5932..6624)
                     /locus_tag="PLB01_14725"
     CDS             complement(5932..6624)
                     /locus_tag="PLB01_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78607.1"
                     /translation="MAPDGAPLLAAEGLAKGFGSGAGRVDVFRNVSLEVRAGELLAIT
                     GPSGCGKSTLLHLFAGLDTPDAGAVRVSGTDWNSLSPEARAALRGTTIGFVFQFHHLL
                     PELTAEENVALPLFLAGRSEADGRKEAREVLDRVGLSSRASALPRTLSGGERQRVAIA
                     RAVVRRPRLLLCDEPTGSLDAAHAAAVFDLLTTVARERAGAAIVVTHDPEWARRCASI
                     KSLGGEGLSDGA"
     gene            complement(6614..>6870)
                     /locus_tag="PLB01_14730"
     CDS             complement(6614..>6870)
                     /locus_tag="PLB01_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HQQ78608.1"
                     /translation="GACVGAAGTLGGLALGVGAAVLLERAGLVPLPTQLYGMAHAPFR
                     VEALDVLAVGVLSILWSFLAASIPARTAARLSPVEALRGA"
BASE COUNT         1009 a         2203 c         2327 g         1231 t
ORIGIN      
        1 agcggccggt gcgggcgcgg cggcggcggc cggcttcgcg gcggcggcct tcggcgcggc
       61 gctctgcgag ttgaatttcg tgatgacggc ctccgtcagg tcgacggact tgtcgtccgc
      121 aaagaggaga ccgctgttga acttgttgaa gacgagctgg tagccctgct cccgggccac
      181 ctcgttgatg acgggcatga tccgcttctc gaggtcgccg agttccttgc gctgggattc
      241 ctccagctcc ttctgggcgt cgtcctggaa gcgcttgagc tcgaccgcct tgccctggta
      301 ctccttctgg agcttctcca tggcctcctc ggacagggcc ttgccctggt cctggatttt
      361 cgtctcgagg tcgcgaagat ccttctggcg cttcgtgagc tcttcctgct tcgtctgctg
      421 gagcttctgg acacgggcca ggctctcctt gccgatggcc gactcctgga cgatccgctg
      481 gacttcgatg acgccgatct tcgatccgga ggcggcctga gaggtggcgg gggccgcagc
      541 cgtctgcgcg aaggccggga cggaggcgac ggcgagggcg agtgcgccgg ccgcgatcgt
      601 gacgatgcgc ttcatggaat ctgactcctt aaggtgtttt ccgggggccg gaggccggcc
      661 gagcgccgga tgatagcaat tcgagggctc ggatcagaaa gtcgtcccga tggagaactg
      721 gaagtccgtc ttgctctcct cgccgttggc caggggcttc ccggtctggt cgaggatgac
      781 cttcggctgg aggttgatgc cgtagatgaa gcgcaggggg gcctggaaga tcggcaggaa
      841 gatccgcatc tcgaggccga ccgcagtcct catcttgagg agattcaccg tctggccctc
      901 gatccaggtg ttccccgcgt cgaagaacgc cacgagcttc agcggctggg cgagctggta
      961 cacgtactcg aggttgaaga cgacgaaacg gttcccgccc aggagggcgc cgttctggtc
     1021 gaagaacgcc cggttctcgt cgtcgagcgg gaagatcgct ccgtacttga aggcgcgcac
     1081 ggaccggtcg ccgccgatcc ggtagcgttc gaagatcggg atctcgctgc gcccggcctc
     1141 gcgctgagcc tggctgtcgt acgggaacag catcccgagc tcgaagttca cggcgaagtt
     1201 cgacttcttc gtggccggca ggtaggccgt gacgctggcg gcgctcttca cgaacgagaa
     1261 gtcgcccccg agcgcgccgc ccgcgatcgt caccgaggcg ccgtagcgca acccgcgcgt
     1321 cgggtcgaag gggtcgttgc ggctgtcgaa gcggtagccc ggagtgatcg aggacgtctt
     1381 gccggtgtag tccgcgaacg cgggcggcgt gtaacccccg cccggaggag gaggcggcgg
     1441 gtacacctga tagcggctct tcgtgtccgt gaacccgtag aagagggaga cgacgtccca
     1501 cacgccgagc ccgacgccga gcgaaaggtt caggcctttc gagctctggt tcgtgccgcc
     1561 ggcgacgtcg ctgtagtcga gcgtgcggtt gaagatcgcc ccgccgacgg acatccgctg
     1621 gtcgaggaac cagggctcgg agaacgagac gtcgaagaag ttcgaccggt tgccgcgctg
     1681 gaactgcagg ccgagcgtgt cgccccggcc gaggaaattg cgcgtgttga actggaactg
     1741 cccgaagaga ccgtcgagct gcgagtaacc ggcgccgaac tggacctcgt tgcggttcgt
     1801 gtccgtgccc ttgatcgtga tgtcgaccgt cttcttgtcg ttgttgacct tgaagtcggg
     1861 gtcttcctcg accttgaagt agcccagctg cgtgaccttc tggatgctct tcttgaaggt
     1921 ctccatgtcc atgatgtcgc cttcgtagag ctgcagctcc cggcgcagga ccttgtcgcg
     1981 cgtcgtcttg ttgccctgga actcgacgcg tccgagccgg tactggtcgc cctcggcgat
     2041 cttcaccgtg acgtcgacga cgtgattcgg cttctccgtg aactccgggt tcatgaacac
     2101 gtagatgtag ccctggctgc ggtagatcgc ctcgatcgac ttcattccgt ccgcgaggat
     2161 ggcgcgggag agcggcttgc ccgtgcggta cccgaagaac ttctcgagga tctccggctt
     2221 gtagaccgtg gacccgtcga cgttgagctt gccgaagaac cagcggtcgc cctcgacgat
     2281 cgggatcgtg agcctcgcgc ggcgcttgat cttgtcggga cgcttttcct tcggattcac
     2341 gacgaaggtg tcgatgatgg cgtccttgac gacgacgtcc ttgtatccca cgtcctggta
     2401 ggccttgcgg aggctctcga cgtcctcgtc gtagctcgcc tggttgaacg tggacttgct
     2461 gtcgaagaaa cgccaccagt gggcggtcgc cgtcttcttc attgcgttgc gcagcgtctg
     2521 ggcgccgaag acggtgttcc cggtgaagtt gatcgactcg accttcatct tgtcgccttc
     2581 gtccacgttg aagacgacgc gccgttgcgc ggccgagacg atctcgagct gcgcctcgac
     2641 gacggcggac cggaagccct cggccttgta gccctcgagg aggacgccct tcgccttggc
     2701 cacgtcccgc agggagacgg gggcgccgat ccggatctcg gccttgcctt ccttgagctt
     2761 gtccttgagc tgcgaggtcg tgagcttctt gttgccgcga aactcgaggt ccgcgatcgt
     2821 cggacgttcg accaccgtcg ccgtgaggac gacgccctgg ggcgtctcgt ccttctcgag
     2881 cgtgacgtcc tcgaagagtc cggagtccca gagcttcgag aagttccgct tgacgagctc
     2941 gtcgtcgtac gggtcgccgg gcttgaggcc gaggtagaac ccgacggtct cggacgcggt
     3001 cgtctttccg cccttgacga cgatctggac gagccgggga agcttctggg cggccccctt
     3061 ctccccttcc gacgcgagcc ggaccgacgg agcggccccc atggcgcccg agagcggcga
     3121 ctcggtcttc gggccgaagg cggcgtcggg agcgggggtc ggcgcggggg ccggctgcgg
     3181 ggcctgcgcc ccggcgggcc cggcgaggag gagtgcgccg aaaaggcctg cgcccgcgag
     3241 ggcgcgaatc gagcgtacgg cactcccggc ggaggcgccg gaaaggagac aaggcctcaa
     3301 ggcgcttacc ggcccgtccc gacgggctct ggcgcccgga tctggaccct gagctcgtcc
     3361 ccttccacgg tcacttcgac gcactcgata ggctctcggg acgcgatcag caattccgat
     3421 accgcatcct cgacgtgccg ctggatcgtc cgccggaggg ggcgggcgcc ggagtgggcg
     3481 tcgtggccgg aggccttgag cagccagtcg accgcggcct ggctcgcgac gagcttgagc
     3541 ccgcggtgcc ggagcgtctc ggcgacgtcg tcgaggagca ggcggccgat cccgcgcagc
     3601 tgctcgagac cgagcgggtt gaagacgatg acctcgtcga tgcggttgat gaactccggc
     3661 gagaactccc tccgcagctc cttgaggacc atctcctcga cctcgcgctg ctcgggagcg
     3721 ttcttgccct cgccgaagcc catgcggctt ccggtcacga ggtacttcgt cccgacgttc
     3781 gaggtcatga tgatgagcgt gttcttgaag tcgacgacgt ttccgtacgc atccgtcagc
     3841 tgcccgtcct cgaggatctg caggagcagg ttcgcgatgt ccggatgcgc cttctcgatc
     3901 tcgtcgagga ggatcacgga gtagggctgg cggcggatgc gctccgtgag ctgcccgccc
     3961 tcctcgtggc cgacgtagcc cggaggcgag ccgatcagct tggagaccgc gtgcttctcc
     4021 atgtactcgg acatgtcgaa gcgcacgagc gccttctggt tgtcgaacag gaactcggcg
     4081 agccggcgcg cgacctcggt cttcccgacg cccgagggcc cgaggaagat gaacgagccc
     4141 atgggccggt tcgggctctt cacgccgagc cgcgagcggc ggatcgcgcg gctgatcgcg
     4201 ccgatcgagc ggtcctgccc gatcacgcgc ttcttgagcg tctcctccat gtggaggagc
     4261 ttctcggcct cttccatctg gagcgacgtc acggggatcc ccgtccagct cgagataacg
     4321 tcctcgatgt cggcgcgcgt cacgtcgacg gccgcggact cgtcctgggc cgccgtcttc
     4381 gtccgctcga tctcttcctt gatctcgatc tcgcgctcgc gcaggaacac ggcgcgctcg
     4441 aagtccttct cggagatcgc cttcttcatc tccttgacga tccccttgat ctcgttctcg
     4501 agccggcgca ggttctgcgt gtccgccgtc ttgcggagct tcaccttcgc gccggcctcg
     4561 tcgaggatgt cgatcgcctt gtcggggaag aagcggtcgg ggatgtagcg gctcgcctgg
     4621 tagacggcgg tcttgatcgc gtcgggcgtg tagcggacgc ggtggaactt ctcgtaccgt
     4681 tcgcgaacgc cttccaggat ctgcagcgtc tcttcctcgt ccggcggagc caccgtgacg
     4741 gcctggaagc gccggaggag cgagcggtcc ttctcgatgt acttgcggta ctccttgagc
     4801 gtcgtcgcgc cgatgcacga gatctcgccg cgcgagagcg ccggcttgag gatgttggcg
     4861 gcgtcgagcg agccctcggc cgagcccgcg ccgatcaggg agtggatctc gtcgatgaag
     4921 acgatgaggt cctggttctc cttcagctcc ttgaggatcc ccttnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnncgtcga tgaagacgat gaggtcctgg ttctccttca
     5101 gctccttgag gatccccttg aggcgctcct cgaactggcc gcggtacttc gtgcccgcca
     5161 cgatcagcga gagatcgagc gcgaggatct tcctctgcgc gaggaacagc gggacgccgc
     5221 cctcgacgat cttctgcgcg aggccctcga cgatcgcggt cttgccgacg cccggctcgc
     5281 cgaggaggat cgggttgttc ttcgtgcggc gcgagaggat ctggacgatc cgctcgacct
     5341 ccgcgtcgcg cccgaccagc gggtcgaacg atccggtttg tgcgagcgcc gtgaggtcgc
     5401 gcgagtattc ggcgaggaac gggagctcct tcttctgctt ggaggcctcg cgctccttga
     5461 tgagcgcgat cgtctcttcg cggacgccgt agaggttgaa gccgtgcgcc atgaggatcc
     5521 ggcccgcgac gcacgggtcg acgcggagga tgccgatgag gagatgctcc gtgccgacgt
     5581 aggggtgcat catcgactcc gcctcgtgcg aggcgtaggc gaggatcttc ttggactcct
     5641 ccgagagcgg aagctcctgg gtcgaggaga cgcgctcgac gaacacgcgg tcgccttcga
     5701 tctcgtgccg gatgtcctcg ggtttgacgt tgaagcgcgc gaagatctcc ttgatgacgt
     5761 cctcgccctc ccggaggatt ccgaggagga tgtgctcgga ctcgatgacc ttcgagccca
     5821 gcttcgagga ctcgtaacgg gcgaagaaca gggcccgccg cgccttctcg ttgtatttct
     5881 cgaacatcgt tatgactgcc gtttcgggcc ggaaaaaggg tcgagccgcg cctacgcgcc
     5941 gtcgctcaag ccttctcctc ccaacgattt gatgctagca cagcgacgcg cccattccgg
     6001 gtcgtgcgtg acgacgatcg cggccccggc gcgctcccgg gcgaccgtcg tgaggaggtc
     6061 gaaaacggcc gcggcgtggg ccgcgtcgag gcttcccgtc ggctcgtcgc agaggaggag
     6121 gcggggccgc ctcacgaccg cacgcgcgat ggcgacgcgc tgccgctcgc ccccggacag
     6181 cgtccgcggg agggccgacg cacgcgacga gaggccgacg cgatcgagca cctcgcgggc
     6241 ctctttccgg ccgtccgcct cggaccggcc ggccagaaag agcggcagcg cgacgttctc
     6301 ctccgccgtg agctccggta ggagatggtg gaactggaac acgaacccga tcgtcgtccc
     6361 gcgcagggcc gcgcgagcct ccggggacag ggaattccag tcggtcccgg acacgcgcac
     6421 ggcgcccgcg tcgggcgtgt cgagcccggc gaacaggtgg aggagcgtcg acttcccgca
     6481 gcccgacggt cccgtgatcg cgagaagctc gcccgcgcgc acttcgaggg agacgttccg
     6541 gaacacgtcc acgcgcccgg cgccggagcc gaaacccttc gcgaggccct cggccgcgag
     6601 gagcggcgcg ccgtcaggcg ccacgcagcg cctccacggg cgagagccgc gcggccgtcc
     6661 gcgccgggat cgaggccgcg aggaacgacc agaggatcga gaggaccccc acggcgagga
     6721 cgtccagggc ctcgacgcgg aacggcgcgt gcgccatccc gtagagctgc gtgggcagag
     6781 gcacgagccc cgcacgctcg aggagcaccg cggctcccac gccgagggcg aggccgccga
     6841 gcgtccccgc cgcgcccacg caggcccccg
//
LOCUS       DAOMFY010000214         6868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3612_length_6868_cov_6.761926, whole genome shotgun sequence.
ACCESSION   DAOMFY010000214 DAOMFY010000000
VERSION     DAOMFY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3612_length_6868_cov_6.761926"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLB01_14735"
     CDS             <1..347
                     /locus_tag="PLB01_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQQ78609.1"
                     /translation="ESGPASLALPPAVAAATVALPVDAPGVSSGALARSGRTLTLSFR
                     DECWAEVFVDGKVVVRGLFPKGAKREFSGGGTFTLTLGNAGVVEVAVDGRSLDPIGGE
                     GEVVKNYVINHG"
     gene            367..1389
                     /locus_tag="PLB01_14740"
     CDS             367..1389
                     /locus_tag="PLB01_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78610.1"
                     /translation="MSFVPDDLLGQVESGEASSQILDFVARGLVPLPAEELVRAVASI
                     LAAGDAALSASAEATFAGFHSEDLKGAVLSGGVRPEQLDAIARRTSEHTVLEPLIRHK
                     AVPDATLAWLAERVDGPMQDIIVTNQVRLLEAPVIVERLFENPRLSPEIRRRADEFLE
                     EFFLKKERERDEAAAAAAAAPEAAPEPAPEPAPAEDEPVLSEDEKKSLFARVSVMTVV
                     QRIKLAYRGNKEERMFLVQDRNRLVSAAALKSPKTREADAETIANMKSVSEDVLRGIA
                     FRRDWMRHYSVVRALVRNPRSPIDVTLGLVSKLNAKDMKMLAGDRNVPEAVRATARRE
                     VARKET"
     gene            1477..2133
                     /locus_tag="PLB01_14745"
     CDS             1477..2133
                     /locus_tag="PLB01_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQQ78611.1"
                     /translation="MTNYYDVLGVARDATAAEITNKFRALAREQHPDRYSDLDRKRSA
                     ESKFQILTEALNVLTNPARRKVHDAELEKSRPVSQDPAGQARVFLAKGVKSYREGNFP
                     DAIQQFELAVNHYDKDAKAWHYLALSCMKVVGQTRKGVDAIEAAIKLDPNNALMRRDA
                     GKLYLMAGLPSKAERHLEEALTWMPEDPETLTLLMKVRPDRPTSGVRKPPSGIFGRKG
                     "
     gene            2141..3022
                     /locus_tag="PLB01_14750"
     CDS             2141..3022
                     /locus_tag="PLB01_14750"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HQQ78612.1"
                     /translation="MVVSAFGLTDVGRKRRHNEDAYLIDEERGLFVVADGMGGHAAGE
                     VASRITVESIQEYIAATEEEHESSWPFGFNSRVSLEGNRLTTAVEKANEKVMRAVQNR
                     PELKGMGTTVVAALFDGDRATLVHVGDSRAYLFRDGELRRLTDDHSWVQEQVNAGILS
                     EDEAKSHPLKNVVTRALGGAAHVSVDLIEVPVRTGDRYLLCSDGLTGMLPDEELFEHF
                     RTSGSLDTTVRMLIEVANARGGVDNITAILVEVKDAGSGKVDAAKTDPPRSKALSNES
                     TAVTIDGPPPKVNPAGS"
     gene            complement(3003..5480)
                     /locus_tag="PLB01_14755"
     CDS             complement(3003..5480)
                     /locus_tag="PLB01_14755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQQ78613.1"
                     /translation="MALTIDLAAKTIALSPGALLAGSGRRIGFDRGEGFERLWIGQAV
                     HRRVLGGNLETVPGYAVEKAIRLSFTVDDYAAVLEGRLDGRWEDGGRVVVDEVKSLHF
                     AEDLARLPGSPRLERFQLQLRWYMHAVAEAEGREVTGRLILADIETGATKIIEVAHEP
                     EKTLSDLVRRTRLLLDEFLEERALAEAKAAEAGTLVFPHESYRLGQSEMEDAAARAAA
                     HGEHLLAEAPTGIGKTAAAVFPLLKAALDSGRRLYVLTAKTTQQEIYEKTIGLVEGDS
                     FRSVRLRAKERMCANDVVLCHEDHCPYAKDYGAKMEASGLLERLVETHRHLDPDTVFE
                     ESRAETVCPFEVSLELAERADVVIGDYNYVFDPVVALSGAREPAALKDAFLLVDEAHN
                     LVDRGRGYYSPELAEIELRTLEQRIGASTAKTAWDAGEAVRRILKLVSESASILPEEE
                     REAADLVTLDEARLDDLRLDMESLLVRHLAEMRSGGERVPDDPVLDLYFTYARFHDVS
                     KQAAAGDKAAEAFDILASRGPRGAKLAILCKDPSRLLGETLNAAAATVSMSATLSPPE
                     FYRDLLGLDADRTTVLRLPSPFPRENRAILIAPDVDTTYAARGKGAPRIAALVADVAR
                     GCPGNVLALFPSYRFLDDVRAHLPALPGRRVVRPSDRSTEVERNRVLASLKARGAPVL
                     LLAVSGGAFAEGVDYPGEMLRAVVVVSPALPMVRFEQERMRRYFEERYERGFEYAYVI
                     PGMTRVVQSAGRLIRSESDTGVVVLACRRFLQAPYSRYLPEHWYLDDPSELACIDVGR
                     STREFFEKVGVGRPALQSTPDVRSPRD"
     gene            5541..>6868
                     /gene="dacB"
                     /locus_tag="PLB01_14760"
     CDS             5541..>6868
                     /gene="dacB"
                     /locus_tag="PLB01_14760"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HQQ78614.1"
                     /translation="MRLVARRSVACAAAVAAVFVALPGAAATKKKAPSAPATLEQRLL
                     AIARRPPVRRSEAGIYVARVGEAKPLVAFNTERPLILASTTKLFTSAAALDRLGPAYR
                     FRTRLYRDAEIGVDGSLPGHLVVVGGGDPGLSGRWYDDDPLAVFRPWAQSLVKRGLRD
                     VKEGLVLDASFFDDVQVHPDWPSEQEARWYQAPVSALSYNDNVVLVRASGGVRPGAPA
                     ILGFDPLGPPLLNLISQVVTAQRRTYVGVRREAGSHTVVAAGAVGRNRTWTGDVTVPD
                     PPLYFASALAQVLRENGIQLSAPPTVRTEPARPGPHVLLHTHETPILPVLAVCNKRSQ
                     SFFAEQILKTLGAETRGKGSWENGRAEVKAFLASLGLDPQRYDLADGSGRAHTNKASA
                     GAYGDFLQALATKWSHFEEFKSTMAVSGEPDGTLRHRLQTEATRGKVFAKT"
BASE COUNT         1056 a         2398 c         2459 g          955 t
ORIGIN      
        1 ccgagagcgg gccggcgtcg ctcgcgctcc cgccggcggt cgccgcggcg acggtcgcgc
       61 tccccgtgga cgcgccgggc gtctccagcg gagcgctggc ccgatcgggc cggacactca
      121 cgctgagctt cagggacgag tgctgggccg aggtgttcgt agatggcaag gtcgtcgtgc
      181 gcgggctctt cccgaaggga gccaaacggg agttctcggg aggcggcacg ttcacgctca
      241 cgctcggcaa cgccggcgtg gtcgaggtcg ccgtcgacgg ccgatcgctg gacccgatcg
      301 gcggcgaggg cgaagtcgtc aagaactacg tcatcaacca cggctgaccg gcgctaatct
      361 ccctccatgt cgttcgtccc cgatgacctg ctcgggcagg tcgagtccgg cgaggcatcc
      421 agccagatcc tcgacttcgt cgcgcgcggc ctcgtgccgc tgcccgccga ggaactggtt
      481 cgcgccgtcg cgagcatcct cgcggccggc gacgcggccc tgtcggcgag cgccgaggcg
      541 acgttcgccg gcttccactc ggaggacctg aagggcgccg tcctcagcgg gggagtccgg
      601 ccggagcaac tcgatgcgat cgcgcggcgg acgagcgagc acacggtcct cgaaccgctc
      661 atccggcaca aggccgtgcc cgacgcgacg ctcgcgtggc tcgccgagcg cgtcgacggg
      721 ccgatgcagg acatcatcgt cacgaaccag gtgcgcctcc tcgaggcgcc cgtcatcgtg
      781 gagcggctct tcgagaaccc gcgtctgtcg cccgagatcc gccgccgcgc ggacgagttc
      841 ctggaggagt tcttcctcaa gaaggagcgc gagcgcgacg aagccgcggc cgcggcggct
      901 gccgctccgg aagccgcgcc ggagccggcg cccgagcccg cgcccgccga ggacgagccc
      961 gtgctctccg aggacgagaa gaagagcctc tttgcgcgcg tctccgtgat gacggtcgtg
     1021 cagaggatca agctcgccta ccggggcaac aaggaggagc ggatgttcct cgtgcaggac
     1081 cgaaaccggc tcgtgtcggc ggcggccctg aagtccccga agacccgcga ggcggacgcc
     1141 gagacgatcg cgaacatgaa gagcgtgtcg gaggacgtcc tgcgggggat cgcgttccgg
     1201 cgcgactgga tgcgccacta ctccgtcgtg agggcgctgg tccggaatcc ccgctcgccg
     1261 atcgacgtga cgctggggct cgtgtcgaag ctgaacgcca aggatatgaa gatgctcgcg
     1321 ggcgaccgga acgtcccgga ggccgtgcgg gccacggcca ggcgggaggt ggcgcgcaag
     1381 gagacgtgag cctccttgaa atgacgcgcc ttttcgtgag aatctctcgg gtcaagtgac
     1441 cggtcagacg cggcctccgc gccttttgag gtgtcggtga cgaactacta cgacgtgctc
     1501 ggcgttgcgc gggacgcgac cgcggctgag atcacgaaca agttccgcgc gctcgctcgc
     1561 gagcagcacc cggaccggta ctcggacctg gaccgcaagc gctccgcgga atccaagttc
     1621 cagattctca ccgaggccct gaacgtcctg accaacccgg cgcgccggaa ggtgcacgac
     1681 gccgagctcg agaagagccg ccccgtcagc caggaccccg cggggcaggc gcgcgtcttt
     1741 ctcgcgaagg gcgtcaagtc ctaccgggaa gggaacttcc ccgacgcgat ccagcagttc
     1801 gagctcgcgg tgaaccacta cgacaaggac gcgaaggcct ggcattacct cgcgctctcg
     1861 tgcatgaagg tcgtcgggca gacgcgaaag ggcgtcgacg cgatcgaggc cgcgatcaag
     1921 ctcgacccga acaacgcgct catgcggcgc gacgccggga agctctacct catggccggg
     1981 ctgccgtcca aggccgagcg ccacctcgag gaggcgctca cctggatgcc cgaggacccc
     2041 gagaccctca cgctgctcat gaaggtgcgc ccggacaggc cgacgagcgg cgtccgcaag
     2101 ccgccgagcg ggatcttcgg gcggaaggga taatcgcggc gtggtcgtct ccgcattcgg
     2161 gctcaccgac gtcggccgga aacggcgtca caacgaggat gcgtacctca tcgacgagga
     2221 gcgcggcctc ttcgtcgtcg cggacgggat gggcgggcat gcggccggcg aggtcgcctc
     2281 gcgcatcacg gtcgagtcca tccaggagta catcgccgcc accgaggagg agcacgagag
     2341 ctcgtggccg ttcggtttca acagccgcgt ctcgctcgag ggcaaccgcc tcacgacggc
     2401 cgtcgagaag gcgaacgaaa aggtcatgcg cgccgtccag aaccgtcccg agctgaaggg
     2461 catggggacg acggtcgtgg cggcgctctt cgacggcgac agggccacgc tcgtccacgt
     2521 cggcgacagc cgggcctatc tcttccgcga cggcgagctg cggcgcctga cggacgacca
     2581 ttcctgggtc caggagcagg tcaacgccgg gatcctgagc gaggacgagg ccaagagcca
     2641 cccgctcaag aacgtcgtga cgcgggcgct cgggggcgcc gcgcacgtct cggtggacct
     2701 catcgaggtc ccggtgcgca cgggagaccg ctacctcctt tgctcggacg ggctcacggg
     2761 aatgctcccc gacgaggagc tcttcgagca cttccggacg tcgggttcgc tcgacacgac
     2821 ggtccgcatg ctgatcgagg tcgcgaacgc gcgcggcggc gtcgacaaca tcacggcgat
     2881 cctcgtcgag gtgaaggacg cgggctccgg aaaggtcgac gccgcgaaga ccgacccgcc
     2941 ccggtcgaag gccctgtcca acgagagcac cgccgtcacc atcgacggcc ccccgccgaa
     3001 ggtcaatccc gcgggctcct gacgtccggc gtcgactgga gcgcgggcct tcccactccg
     3061 accttctcga agaactcccg ggtgctcctg ccgacgtcga tgcaggcgag ctccgaaggg
     3121 tcgtcgaggt accagtgctc cgggaggtac cggctgtaag gcgcctggag gaaccggcgg
     3181 caggcgagga cgacgacgcc cgtgtccgac tcggaccgga tgagccggcc ggcgctctgc
     3241 acgacgcgcg tcatccccgg aatgacgtag gcgtactcga acccgcgctc gtatcgctcc
     3301 tcgaagtaac ggcgcatgcg ttcctgctcg aagcgcacca tcgggagagc gggcgagacg
     3361 acgacgaccg cccggagcat ctctccggga tagtcgacgc cctcggcgaa cgcgcctccc
     3421 gagacggcca ggaggaggac cggggcgccc cgggccttca gggaggcgag gacgcggttg
     3481 cgctcgacct ccgtggagcg gtcggacggg cggacgacgc ggcgccccgg cagcgcgggc
     3541 aggtgggcgc gcacgtcgtc gaggaaccgg taggacggga agagcgcgag gacgtttccc
     3601 ggacagccgc gcgcgacgtc cgcgacgaga gccgcgatgc gcggcgctcc cttgccgcgc
     3661 gccgcgtacg tcgtgtcgac gtccggagcg atcaggatcg cgcggttctc gcgcgggaac
     3721 ggcgagggca ggcgcaggac cgtcgtgcgg tccgcgtcca gcccgaggag gtcgcggtag
     3781 aactcgggcg gggagagcgt cgcggacatc gagaccgtcg cggcggccgc gttgagcgtc
     3841 tcgccgagga ggcgcgacgg gtccttgcag aggatcgcga gcttcgcgcc ccgcggcccc
     3901 cgcgaggcga ggatgtcgaa cgcctcggcg gccttgtcgc ccgcggcagc ctgtttcgac
     3961 acgtcgtgga aacgcgcgta cgtgaagtag aggtcgagga ccgggtcgtc cggcacgcgc
     4021 tcgcccccgg agcgcatctc ggcaaggtgg cgcacgagga gggattccat gtcgaggcgc
     4081 aggtcgtcga gccgggcctc gtcgagcgtc acgaggtccg ccgcctctcg ctcctcctcg
     4141 ggcaggatcg acgcggactc cgagacgagc ttgaggatgc ggcgcaccgc ctcgccggcg
     4201 tcccacgccg tcttcgcggt ggaggcgccg atcctctgct cgagcgtccg gagctcgatc
     4261 tccgcgagct cgggcgagta gtagccgcgg ccgcggtcca cgaggttgtg cgcctcgtcg
     4321 acgagaagga atgcgtcctt gagcgcggcc ggctctcgcg ccccggagag ggcgacgacg
     4381 gggtcgaaca cgtagttgta gtcgccgatc acgacgtccg cgcgctcggc gagctccagc
     4441 gaaacctcga acgggcagac ggtctcggcg cgggattcct cgaagacggt gtccgggtcg
     4501 aggtgccggt gcgtctcgac gagccgctcg aggaggccgg aggcctccat cttggcgccg
     4561 tagtccttcg cgtacggaca gtggtcctcg tggcagagga cgacgtcgtt cgcgcacatc
     4621 cgctccttgg cgcgcaggcg gacggaccgg aaagagtcgc cttcgacgag gccgatcgtc
     4681 ttctcgtaga tctcctgctg cgtcgtcttc gcggtcagga cgtagagcct gcggccggaa
     4741 tcgagggcgg ctttgaggag cgggaacacc gcggcggcgg tcttcccgat gcccgtgggc
     4801 gcctcggcga gcaggtgctc gccgtgcgcg gcggcgcgcg cggccgcatc ctccatctcg
     4861 ctctggccca ggcggtagct ttcgtgcgga aagacgagcg ttcccgcctc ggcggccttc
     4921 gcctcggcga gcgcgcgctc ctcgaggaac tcgtcgagga ggagccgcgt gcgccggacg
     4981 aggtccgaca gggtcttttc gggctcgtga gcgacctcga tgatcttcgt cgcgcccgtc
     5041 tcgatgtccg cgaggatgag gcgccccgtg acctcccggc cctcggcctc ggcgaccgcg
     5101 tgcatgtacc agcgcagctg gagctggaag cgctcgaggc gaggcgaccc gggcagccgc
     5161 gcgaggtcct cggcgaagtg cagcgacttc acctcgtcca cgacgacacg gccgccgtct
     5221 tcccagcgcc cgtcgagccg gccctcgaga acggccgcat agtcgtcgac cgtgaaggaa
     5281 agccggatcg ccttctccac cgcgtagccc ggcacggttt cgaggttccc gccgaggacg
     5341 cgccggtgga cggcctggcc gatccagagg cgctcgaagc cctcgccgcg gtcgaacccg
     5401 atgcgccgcc cgcttccggc gagaagcgcg cccggagaga gcgcgatggt cttcgccgcg
     5461 aggtcgatcg tgagcgccac gcgcgattgt agcggcgcaa ggggctatcg gggacgctcc
     5521 ggaccggcta aaattcctgc atgaggcttg tcgccaggcg gagcgtcgcg tgcgcggcgg
     5581 cggtcgccgc cgtgttcgtc gcgttgcccg gcgccgcggc gacgaagaag aaggctccgt
     5641 cggctccggc gacgctcgaa cagcgccttc tcgcgatcgc gcgccgcccg cccgtgcgtc
     5701 gctccgaggc gggcatctac gtcgcgcggg tcggcgaggc gaaacccctc gtcgccttca
     5761 acacggagcg cccgctcatc ctcgcgtcca cgacgaagct cttcacgtcg gccgcggcgc
     5821 tcgaccggct cggcccggcc taccggttcc ggacgcggct ctatcgcgac gccgagatcg
     5881 gggtcgacgg gtctcttccg ggccacctcg tcgtcgtggg cggcggagat cccgggctct
     5941 ccgggcgctg gtacgacgac gacccgctcg ccgtgttccg cccctgggcg cagtccctcg
     6001 tgaagcgcgg cctgcgtgac gtgaaggaag gcctcgtcct cgacgcgtcg ttcttcgacg
     6061 acgtgcaggt gcaccccgac tggccctcgg agcaggaggc gcgctggtac caggcgcccg
     6121 tctcggctct ctcgtacaac gacaacgtcg tcctcgtgcg cgcctcgggc ggggtgcgtc
     6181 ccggcgcgcc cgcgatcctc ggcttcgacc cgctcggccc gccgctcctg aacctcatct
     6241 cgcaggtcgt cacggcgcag cgccggacgt acgtcggcgt gcgccgcgag gccggcagcc
     6301 acaccgtcgt ggccgccggc gccgtcggaa ggaaccggac ctggacgggc gacgtgacgg
     6361 tgccggaccc gccgctctac ttcgcgagcg cgctcgcgca ggttctgcgg gagaacggga
     6421 tccagctctc ggctccgccg acggtccgga ccgagccggc gcggcccggc ccccatgtcc
     6481 tgctccacac gcacgagacg ccgatccttc ccgttctcgc ggtctgcaac aagcgctcgc
     6541 agtccttctt cgccgagcag atcctcaaga ccctcggagc cgagacccgc gggaagggct
     6601 cgtgggagaa cggccgtgcc gaggtgaagg ccttcctcgc ctccctgggc ctggacccgc
     6661 agcggtacga cctcgcggac ggctcgggcc gcgcgcacac gaacaaggcg agcgcgggcg
     6721 cctacggcga cttcctgcag gccctcgcga cgaagtggag ccacttcgag gagttcaagt
     6781 ccacgatggc cgtgtcgggc gagcccgacg gcacgctccg ccaccgcctc cagacggagg
     6841 ccacgcgcgg gaaggtcttc gcgaagac
//
LOCUS       DAOMFY010000215         6854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3622_length_6854_cov_6.277247, whole genome shotgun sequence.
ACCESSION   DAOMFY010000215 DAOMFY010000000
VERSION     DAOMFY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3622_length_6854_cov_6.277247"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..744
                     /locus_tag="PLB01_14765"
     CDS             103..744
                     /locus_tag="PLB01_14765"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQQ78615.1"
                     /translation="MVEDTTFRVAIPRADLGQTHLLVGAAARLTWRDPRLPAAWIAAE
                     VLGGGVSSRLWRDVRESRGLAYHVGAHLSMHRMAGLALITAATQPKNLERLVRTTGRV
                     LGRLLRDGVSRAELARAKNQFAAEAALSQESTASRRETAARAWLSRGRPYETEEYLAD
                     VAKVTADGVAEAAALLWGDAARMGLGVSGPLPEAGAERFARALADDFAGEAAA"
     gene            741..1199
                     /gene="dut"
                     /locus_tag="PLB01_14770"
     CDS             741..1199
                     /gene="dut"
                     /locus_tag="PLB01_14770"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQQ78616.1"
                     /translation="MKPGEEVAVRFTVAEEFRDLAPPRYASADAAGADLRAAIAEPLV
                     LAPGARALVPTGITLEIPRGFEAQVRARSGLALKKGLALANGVGTIDADYRGEVGVIM
                     VNLGAEPVTIARGERIAQLVVAPVARAVFMRAGALDGSARGAGGFGSTGS"
     gene            1196..2002
                     /locus_tag="PLB01_14775"
     CDS             1196..2002
                     /locus_tag="PLB01_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ78617.1"
                     /translation="MSAPSGLSVAFLGSGSSGNCAVVRCGKTAVLVDAGLSLRETKKR
                     LALRGVALEDVSGVFLTHEHSDHVTGALALSRKAGVPVYATAGTADAAGFPGPLFADV
                     RTVRGGRDLVIGDLHVRVTSTPHDGVESVCYVFADGAGRRVGMVTDLGHLSRPVLEAL
                     SDCEALGLEANHDEDLLREGPYPPALKRRILSDVGHLSNEAAAQALRALVGPRTRSVT
                     ALHVSRHNNTYPLAERVFREALGELGARAVLAVARHDVPTEWKTVSEEAA"
     gene            1999..2265
                     /gene="purS"
                     /locus_tag="PLB01_14780"
     CDS             1999..2265
                     /gene="purS"
                     /locus_tag="PLB01_14780"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQQ78618.1"
                     /translation="MTVLVKVMLKSGVLDPQGKAIADALHRLSYTSVSDVRAGKLFRI
                     EVDTADPRHAVEVGTQMAAKLLANPVIEDFEVALETAVAPGGSR"
     gene            2262..2984
                     /gene="purQ"
                     /locus_tag="PLB01_14785"
     CDS             2262..2984
                     /gene="purQ"
                     /locus_tag="PLB01_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQQ78619.1"
                     /translation="MTTARAPRVAVVVFPGSNCDEDAAFAARSTLSAEVKMVRHDERA
                     LGDPDLVILPGGFSYGDYLRSGAMARFSPVMESVAAFAAKGGAVLGICNGFQILCEAG
                     LLPGAMLRNAGLRFVCRDVWLRVESKRTPFTLAINRGDVLRMPIAHGDGNWTADPSVF
                     AEMKARDQVVFRYVSADRRQGGAANPNGSLDDVAGICSVEGNVVGLMPHPERAADPLL
                     GGTDGARLFKSFATTRLAMQER"
     gene            3023..3295
                     /locus_tag="PLB01_14790"
     CDS             3023..3295
                     /locus_tag="PLB01_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HQQ78620.1"
                     /translation="MALNIKDPVAERLAAEVAALAGESKTRAVRVALEERRARLSLSG
                     AAAEKSERIRRFLEEEVWPTIPKKFRGKRITKKERERILGYGPHGV"
     gene            3292..3681
                     /locus_tag="PLB01_14795"
     CDS             3292..3681
                     /locus_tag="PLB01_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQQ78621.1"
                     /translation="MILDSSAIVAILLHEDGADELEAKVAAAPALGIGAPTLVETLMV
                     LSSRFGREVAARNLDGFLSRSEAVVVSFSEVHARAAAEAFARFGKGRHRASLNFGDCM
                     SYAVAKLSGQPLLCTGTDFAKTDLKLA"
     gene            complement(3685..4557)
                     /locus_tag="PLB01_14800"
     CDS             complement(3685..4557)
                     /locus_tag="PLB01_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ78622.1"
                     /translation="MARADLRLPANVPGDLYVDSTCIDCATCRQMQPAVFAQGDGHSF
                     VARQPETPGEEARALQALVACPTGSIGTRGRSAGTREASRSFPEPVGDGVFFCGYTSE
                     KSFGAWSWLIRREAGNVLVDSPRAAAPLLARIEELGGVATMFLTHRDDVADHAAFRAR
                     FGAERVIHARDAAALGEPPERTLDGTEPVALADDLLAIPTPGHTRGHAVLLWMDVLFT
                     GDHLAWSARRGRLVAFRDANWYSWPETIRSMEKLLEFDFTRVLPGHGAPYAADSPAAM
                     RREVERCVAWMKTR"
     gene            4756..5844
                     /gene="rsgA"
                     /locus_tag="PLB01_14805"
     CDS             4756..5844
                     /gene="rsgA"
                     /locus_tag="PLB01_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQQ78623.1"
                     /translation="MTLLERFGWSEFFESRFVSFSAQGLVAARVVREERGRLLVVTES
                     GERAAELAGRLLHRAVSRLDLPAVGDWVGLRLGPPGGRSESPAIVEAVVPRRNRIVRK
                     AAGEAVRPQVLAANVDTLLVAMGLDADFNPRRLERYAALAWESEARPLVVLTKADTCE
                     DVPARVAEAEAAAPGAAVVAVSAVTGSGLEALAPHLERGRTVALLGMSGVGKSTLVNR
                     LVGAEAQAVQAVRESDGRGRHTTTRRDLLLLPSGALLVDAPGMRELALWDGGGDAGFP
                     EVAALAERCRYRDCAHEDEPHCAVRAAAAAGELDAQRLESFRKLRKEEAWLARRRDEG
                     LARAEKERWKGLTRGAKERARAKRSGGP"
     gene            5864..>6854
                     /locus_tag="PLB01_14810"
     CDS             5864..>6854
                     /locus_tag="PLB01_14810"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQQ78624.1"
                     /translation="MGAHPDVPRRVIGRLDATMLVVGSMIGSGIFIVSAESARLVGTG
                     GRLLLVWAAAGALTLLAALACAELAAMFPEAGGPYAFFREAYGPMAGFLYGWTMVLVV
                     QTGTIAAVAVAFSKFLGVLAPSVTGWSAKAAAVLVVLVLTATNAVGLKTGTRVQNVLT
                     AVKLASLALLAAACLIAPAAASEPSKNPSSPESPALLVSAFVLALVGPAFSQSAWTNV
                     TFPGGEVKEPGRTFPVALLAGCALVASLYVLTNVGYLNVLGFEGVAHAPDGRVGTAAA
                     ERLLPGAGGAAMAAAILVSTFGCANGLVLSGSRVAWALAQAGFLPEFAARLNAP"
BASE COUNT          881 a         2474 c         2497 g         1002 t
ORIGIN      
        1 ggcgatctca tcgatctaac aagcgcagtc atcgagttcg acatctccga accaggatgc
       61 aggccgctta ttaccgcttc gacaaataat gggaagatca cattggttga agacaccaca
      121 ttccgcgtgg cgatcccgcg cgccgacctc ggccagacgc atctcctggt cggcgccgcg
      181 gcccgcctca cgtggcgcga tccgcgcctt ccggcggcct ggatcgcggc ggaagtgctc
      241 ggcggcggcg tctcgtcgcg cctgtggcgg gacgtgcgcg agtcgcgcgg gctcgcgtac
      301 cacgtgggcg cgcacctctc gatgcaccgc atggccggcc tcgcgctcat cacggccgcg
      361 acgcagccga agaacctcga acggctcgtg cggacgacgg gccgcgtgct gggccggctg
      421 ctccgcgacg gcgtcagccg ggccgagctc gcccgcgcca agaaccagtt cgcggcggag
      481 gccgctctgt ctcaggagtc cacggcgtcg cgccgggaga cggccgcgcg cgcgtggctg
      541 tcgcgcggcc gcccgtacga gaccgaggag tacctcgcgg acgtcgcgaa ggtgacggcc
      601 gacggcgtcg ccgaggcggc ggcgctgctc tggggtgacg ccgcccgcat ggggctgggc
      661 gtctccgggc cactcccgga agcgggggcc gagcgcttcg cgcgcgccct tgcggacgac
      721 ttcgccgggg aggccgcggc gtgaagccgg gcgaggaggt cgccgttcgt ttcacggtcg
      781 ccgaggaatt ccgggatctc gcgccgccgc gctacgcttc ggccgatgcg gcgggagcgg
      841 acctcagggc ggccatcgcc gaaccgctcg tcctggctcc aggcgcgcgg gcgctcgtcc
      901 cgaccgggat cacgctcgag attccgcgag gattcgaggc gcaggtgcgc gcgcgctcgg
      961 gccttgcgct caagaaaggc ctcgcgctcg cgaacggcgt cgggacgatc gacgcggact
     1021 accgcggcga ggtcggcgtg atcatggtga acctcggcgc cgagcccgtg acgatcgcgc
     1081 gcggcgagcg catcgcgcag ctcgtcgtcg cgcccgtcgc acgcgccgtc ttcatgcgcg
     1141 ccggcgccct cgacggcagc gcccgcggcg ccggcgggtt cgggagcacg ggatcgtgag
     1201 cgcgccgtcc gggctgtccg tcgcgttcct cggctcgggg tcctcgggca actgcgccgt
     1261 cgtgcggtgc ggaaagacgg cggtcctcgt cgacgccggc ctctcgctgc gcgagacgaa
     1321 gaagcgcctc gcgctccgcg gcgtcgccct cgaggacgtc tcgggtgtgt tcctcacgca
     1381 cgagcattcc gaccacgtca ccggggcgct cgcgctctcg cggaaggcgg gcgtgcccgt
     1441 ttacgcgacc gccggcaccg cggacgcggc gggctttccc ggcccgctct tcgcggacgt
     1501 gaggaccgtg cgcggcgggc gcgacctcgt gatcggcgac ctccacgtgc gcgtcacgtc
     1561 gacgccgcac gacggtgtcg agtccgtctg ttacgtgttc gcggacggcg ccggccgccg
     1621 cgtcggcatg gtgacggacc tcggccacct gtcccgcccg gtgctcgagg ccctctcgga
     1681 ctgcgaggcc ctcggcctcg aagcgaacca cgacgaggac ctcctgcgcg aggggccgta
     1741 tcccccggcc ctgaagcgcc ggatcctctc ggacgtcggg cacctctcga acgaggcggc
     1801 ggcccaggcg ctccgggcgc tcgtcgggcc gaggacgcgg agcgtgacgg ccctccacgt
     1861 ctcgaggcac aacaacacct atcctctcgc cgagcgggtc ttccgggagg cgctcggcga
     1921 gctcggcgcg cgcgccgttc tcgcggtcgc acggcacgac gtcccgacgg aatggaagac
     1981 ggtctccgag gaggcggcat gacggttctc gtgaaggtga tgctcaagag cggagtgctc
     2041 gacccgcagg gcaaggcgat cgccgacgcc ctccaccgcc tctcgtacac gtccgtctcg
     2101 gacgtgaggg cggggaagct cttccgtatc gaggtcgaca ccgccgatcc ccgccacgcg
     2161 gtcgaagtcg ggacgcagat ggcggccaag ctcctcgcga accccgtcat cgaggacttc
     2221 gaggtcgcgc tcgagacggc cgtggctccc gggggctccc ggtgacgacc gcccgggcgc
     2281 cgcgcgtcgc cgtcgtcgtg ttcccgggga gcaactgcga cgaggatgcc gcgttcgcgg
     2341 cgcggtcgac gctctcggcc gaggtgaaga tggtccggca cgacgagcgc gccctcggcg
     2401 acccggacct cgtgatcctg cccgggggct tctcgtacgg cgactacctg cggagcggcg
     2461 cgatggcgcg cttctcgccc gtgatggagt ccgtcgccgc gttcgccgcg aagggcggcg
     2521 ccgtcctcgg catctgcaac ggattccaga tcctgtgcga ggcgggtctc ctgccgggcg
     2581 cgatgctgcg caacgcgggc ctgcgcttcg tctgcaggga cgtctggctg cgcgtcgagt
     2641 ccaagcggac gccgttcacg ctcgcgatca acaggggcga cgtcctccgg atgccgatcg
     2701 cgcacgggga cggaaactgg acggcagacc cgagcgtctt cgcggagatg aaggcgcgcg
     2761 accaggtcgt cttccggtac gtgagcgcgg accgcaggca gggcggcgcg gcgaacccga
     2821 acgggtccct cgacgacgtc gcggggatct gcagcgtgga gggcaacgtc gtgggcctca
     2881 tgccgcaccc cgagcgcgcg gcggaccctc ttctcggcgg cacggacggc gcgcgcctct
     2941 tcaagtcgtt cgcgacgacg cgcctcgcga tgcaggagcg ctgacggaag ataccattac
     3001 acctttctgg tagagttaca ccatggcgct gaacatcaag gaccccgtgg ccgagaggct
     3061 cgcggccgag gtcgcggcgc tcgccggcga gagcaagacg cgggcggtcc gcgtggccct
     3121 cgaggagcgc cgcgcgcgtc tttccctctc cggggcggct gcggagaaga gcgagaggat
     3181 ccggcgcttt ctcgaggaag aagtctggcc cacgattccg aagaaattcc gcgggaagag
     3241 gatcacgaag aaggagcgcg agcgcatcct cggatacggc ccgcacggcg tgtgattctc
     3301 gattcgtcgg cgatcgtcgc gatcctcctg cacgaggacg gcgcggatga gctggaggcg
     3361 aaggtcgccg cggcgcccgc gctcgggatc ggggcgccga ccctcgtcga gaccctcatg
     3421 gttctctcct cgcgctttgg ccgcgaggtt gccgcccgga acctcgacgg cttcctctcg
     3481 cgctcggagg ccgtcgtcgt ttctttttcg gaggttcacg cccgtgctgc ggccgaggcg
     3541 ttcgcccgat tcggcaaggg gcggcaccgg gcgtccctga acttcggcga ctgcatgagc
     3601 tacgcggtcg cgaagctttc gggccagccg ctcctctgca cggggacgga cttcgcgaag
     3661 accgacctga agctcgcctg accctcagcg cgtcttcatc cacgcgacgc atcgctcgac
     3721 ctcccggcgc atcgcggcgg gggagtccgc ggcgtacggc gcgccgtgtc cggggaggac
     3781 gcgcgtgaag tcgaactcga ggagcttctc catcgagcgg atcgtctcgg gccacgagta
     3841 ccagttcgcg tcgcggaagg cgacgaggcg gccgcgacgc gccgaccacg cgaggtggtc
     3901 gcccgtgaag aggacgtcca tccagaggag gaccgcgtgg ccacgcgtgt ggccgggcgt
     3961 cgggatcgcg aggaggtcgt ccgcgagggc aacgggctcc gtcccgtcga gcgtccgctc
     4021 gggcggctcg ccgagggccg cggcgtcccg cgcgtggatg acgcgctcgg ctccgaagcg
     4081 cgcgcggaac gcggcgtggt ccgcgacgtc gtcgcggtgc gtcaggaaca tcgtcgcgac
     4141 gccgccgagc tcctcgatcc gggcaaggag cggcgcggcc gcgcggggcg agtccacgag
     4201 gacgttgccc gcctcgcgcc ggatcagcca gctccacgcg ccgaaggact tctccgacgt
     4261 gtacccgcag aagaacacgc cgtccccgac gggctcggga aaggaccgcg acgcctcccg
     4321 ggtgccggcc gaccggccgc gcgtcccgat cgagcccgtc gggcacgcga cgagcgcctg
     4381 cagcgcccgc gcctcctccc cgggcgtctc cggctggcgc gcgacgaagg agtgcccgtc
     4441 gccctgcgcg aagacggcgg gctgcatctg ccggcacgtc gcgcagtcga tgcacgtcga
     4501 gtccacgtag agatcgccgg ggacgtttgc ggggaggcgc aggtcggcgc gggccacgga
     4561 tcggaaacgg gacgccggac gcgggtattc cggatgttcg tgtaaagttc tccccgaagg
     4621 cctctgacac acgatcttgt gaccccgctc tgagaacccg gcgcccgagc ggcgccgccg
     4681 agttctcgcg cccgccgcat cggcggctcc gtcgcgcgtg tgccttttct tctttccctt
     4741 caaaggagat ctctcatgac gctcctcgag cgtttcggct ggtcggaatt cttcgaatcc
     4801 cgtttcgtct ccttctccgc tcaggggctc gtcgcggcgc gcgtcgtccg cgaggagcgc
     4861 ggccgccttc tcgtggtgac cgagtccggc gagcgcgcgg ccgagctggc gggacgcctt
     4921 ctccaccgcg cggtctcgcg cctcgacctt cccgccgtcg gggactgggt gggtctgcgt
     4981 ctcgggcccc cgggaggccg gtcggaatcc cccgcgatcg tcgaggcggt cgttccccgc
     5041 cgaaaccgca tcgtccggaa ggccgcgggg gaagcggtcc ggccgcaggt cctcgccgcg
     5101 aacgtggaca cgctcctcgt cgcgatgggc ctcgacgccg acttcaaccc gaggcggctc
     5161 gagcgctacg ccgccctcgc gtgggagagc gaggcgcgcc ccctcgtcgt cctcacgaag
     5221 gcggacacgt gcgaggacgt tcccgcgcgc gtcgcggagg ccgaggccgc cgccccggga
     5281 gcggccgtcg tcgccgtgag cgccgtgacg ggctccggcc tcgaggcgct cgcgccgcac
     5341 ctcgagcggg gccggaccgt cgccctcctc ggaatgtccg gcgtcgggaa gtccacgctc
     5401 gtgaaccgtc tcgtcggcgc cgaagcgcag gccgtgcagg ccgtccgcga gtccgacggt
     5461 cggggccgcc acacgacgac gcggcgcgac ctcctcctcc tgccttcggg cgcccttctc
     5521 gtcgacgccc cggggatgcg cgagctcgcg ctgtgggacg gcgggggcga cgcgggcttt
     5581 ccggaggtcg ccgcgctcgc cgagcgctgc cggtaccggg actgcgctca cgaggacgag
     5641 cctcactgcg ccgtgcgcgc ggcggccgcg gcgggtgagc tcgacgcaca gcgcctcgag
     5701 tccttcagga agctccggaa ggaggaggcg tggctcgcgc ggcggcggga cgagggcctc
     5761 gcgcgcgccg agaaggagcg ctggaaaggc ctcacgcgcg gcgcgaagga gcgcgcgagg
     5821 gcgaaacgct ccggcggtcc ctgatgtaac gtgacgggac accatgggag cgcatccgga
     5881 cgtcccccgc cgggtcatcg gccgcctcga cgcgaccatg ctcgtcgtcg ggtcgatgat
     5941 cggctcgggc atcttcatcg tctcggccga gtccgcacgg ctcgtcggga cgggcgggcg
     6001 cctcctcctc gtctgggccg ccgcgggcgc gctcacgctc ctcgcagccc tcgcctgcgc
     6061 cgagctcgcc gcgatgttcc ccgaggccgg cgggccgtac gcgttcttcc gcgaggcgta
     6121 cggcccgatg gcggggttcc tctacggctg gaccatggtg ctcgtcgtgc agacgggcac
     6181 gatcgccgcc gtcgccgtcg cgttctcgaa attcctcggc gtcctcgcgc cgtcggtcac
     6241 gggctggagc gcgaaggccg ccgcggtgct cgtcgtcctc gtcctcacgg cgacgaacgc
     6301 cgtgggcctg aagacgggga cgagggtgca gaacgtcctg accgccgtga agctcgcgag
     6361 cctcgcattg ctggctgcgg catgcctcat tgcgccggcc gcggcctccg aaccctcaaa
     6421 gaatccctct tcgcccgaat ctcccgctct tctcgtttcc gctttcgtcc tcgccctcgt
     6481 cggcccggcg ttctcgcagt cggcgtggac gaacgtgacg ttcccgggcg gcgaggtgaa
     6541 ggagcccggc cgtacgtttc cggtcgcgct cctcgccggc tgtgcgctcg tcgcctcgct
     6601 ctacgtcctc acgaacgtcg gctacctgaa cgtcctcggg ttcgagggcg tcgcgcacgc
     6661 gcccgacggg cgcgtcggta cggcggccgc cgagcgcctg ctgccgggcg caggcggcgc
     6721 cgcgatggcg gcggcgatcc tcgtctcgac gttcggctgc gcgaacgggc tcgtcctctc
     6781 cggctcgcgc gtcgcctggg cgctcgcgca ggccgggttc ctcccggagt tcgccgcccg
     6841 gctgaacgcc cccg
//
LOCUS       DAOMFY010000216         6846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3626_length_6846_cov_7.423207, whole genome shotgun sequence.
ACCESSION   DAOMFY010000216 DAOMFY010000000
VERSION     DAOMFY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3626_length_6846_cov_7.423207"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PLB01_14815"
     CDS             <1..961
                     /locus_tag="PLB01_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQQ78625.1"
                     /translation="ADAARRGVAVRVLADAFGSSATRRSFWRELEGLGIRVRLYHPLF
                     PFLWYHPYRDHRKILAVDRRVAFTGGMNIGEEYGSSVPPAKHGETWRDTHARLEGPVA
                     WEMAVVFREGWDRAGGAPFEIPPLALPPDDAPTPPGASILVLDSAPGRGHEETASVLS
                     AAIAASRERFWLTNAYFAPRRDALPLLAEAARRGVDVRLLLPGVSDVPLVRHAGHGSY
                     RHLLSRGVRIFEYRTAVLHAKSFVADGFLSMVGSTNMDFRSFHFNAECNVVLLDAATA
                     GTLEKQFTADLEDATEITLPDWDRRTRAHRAGDALARMLSPVL"
     gene            1010..1501
                     /locus_tag="PLB01_14820"
     CDS             1010..1501
                     /locus_tag="PLB01_14820"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HQQ78626.1"
                     /translation="MPSRTTILVLWVLSLLPAAGLVGLVVRNDARKADFLRRARGRAD
                     GTVIRLVEQRGKHIQFYPVVAFTTADGRRLEFKSIYSDYLAFGEKHYPLGGRVSVAYV
                     PEDPARAEIDAPALHVKDSGLFVVGGGIYAGLMTLVFGLVALLTKKKRPGSSLPAEPL
                     APS"
     gene            1532..2629
                     /locus_tag="PLB01_14825"
     CDS             1532..2629
                     /locus_tag="PLB01_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78627.1"
                     /translation="MKRRVFSLAAAAVLAGASASAQTITTVAGTGDTAFSPDGSPAAT
                     SSLALATDNVANIVFDSDGNLTFSESRACRVRRIARKTGLLETVAGSGACGYSGDGGP
                     AKEARLRAPAEIAFDAKGNLFVADAGNNLVRRVDRKTGVITTVAGNRLPNYDGDGVAT
                     EVAIGRPSGLVFDRKGRLVISEVYGSRIRRLDFATGKTETIAGKNDLMFTAGPARATG
                     FTWPNGPRYDHSGALFFAASGNNQVLRLDPKGETLTAFAGNGLVGLKGDGGPATEAVL
                     NAPAALAVDGAGNVFIADTGNNVVRRVDAKTGIITLVAGTGAEEFSGDGGPAAAAALS
                     DPLGLGLDGKGNLYVIDARNYRIRRIDGVAR"
     gene            2676..4454
                     /locus_tag="PLB01_14830"
     CDS             2676..4454
                     /locus_tag="PLB01_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQQ78628.1"
                     /translation="MSRRALAASLAAAWLSGCTAGPSASPPSPGPVPGAGRVVAISFD
                     GLGGVRLNELLAAGTLDAGGFAAFAARGVLAGRALDVTPSLTPVAHISAITGAPPAKT
                     GIVANQFREPGSPFGTQTSGFAASIGSETLWEAARRQGRRVGVITYPGADAATEGRRG
                     DFGLVWPEKPARDSAFVSVPGDAWTDAAAAPKSFSPPRAARVAVQTPAGAATLRLVAT
                     DTTDDATANYDLVFVSREDGAAPTRLGAVAVHGWFAISLPGARGSFMAWCRLEELDPS
                     LARAELYVGAFNAVPGYPEDYRRRLEEAIGGWPGVPDYAFVRPPRGDYAAHEEQAARL
                     SEYLTKAILFTIRSERFDLLVGYQPLVDEIEHALEPGPYGGSKEAVVRAFRTADRAAA
                     AILAALGPRDSFLFFSDHGIVPLEAGLNIEKYLLEKGWTPVGPKGPRDGDRRVQICAT
                     SGIAHVYLDPALAPAAHAAAAAALLADLQGLGQLKDGLVDEIVPRADLARLGLDHPRS
                     GDVVVLLKPGTDFWRGGAQILGKPGNRGGHGYRAAYPILDASFGMIGPGIAPARPETV
                     SLLEVAARAANALGIDPPRGAAPAGR"
     gene            4467..5303
                     /locus_tag="PLB01_14835"
     CDS             4467..5303
                     /locus_tag="PLB01_14835"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HQQ78629.1"
                     /translation="MENARVEPAPIAWASLSDVGRVRPANEDYVGDPERLAPLVGGAR
                     RLAARGALFAVADGMGGHARGEIASKLAIDVLYATFYNSPGDPVEMFKKAILTANTAV
                     RRAGVLARPEKSGDGEDARTLPRMGTTLVAALVLGRWVLIGNIGDSRAYLMNDGRLEQ
                     VSRDHSYLAEEIRRGLVPPDAAETSPYRHLITRSLGTKDEVEVDLFWRPWPDGSTLLL
                     ATDGLHGCVEELEMETILASRPPDVAVKELVKAALEAGAPDNVSVVVVHKPVFSRKKE
                     RA"
     gene            5300..5995
                     /locus_tag="PLB01_14840"
     CDS             5300..5995
                     /locus_tag="PLB01_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HQQ78630.1"
                     /translation="MKLFAGTSGFSYDGWTGSFYPEDTPADGRLAYYAAKLPAVEINN
                     TFYRMPKPELLASWAAQVPADFRFALKASQRITHIKRLKEVDGEVKFLLGAARQLGDK
                     LGPILYGLPPNLKKDVPRLAAFLALLPSDAKAAFEFRNPTWLDAEVYALLKSAGAALC
                     VADDEKGTTPLEATAPWGYVRLRREDYGEKDLAAWAERVRAAGWTEAYVFFKHEEEGA
                     APALAARFAEVAG"
     gene            5992..6738
                     /locus_tag="PLB01_14845"
     CDS             5992..6738
                     /locus_tag="PLB01_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQQ78631.1"
                     /translation="MKVATWNVNGIRARELQVLEFLDTHAPDVVCLQELKAAPEQMPI
                     SLAAPAGYASYWHGHKGYSGVGLLLKTATFGETAGFSHPDFDHETRIVEAATPKFSVA
                     SVYVPNGGKDYAAKLAFLEAMADWAHAAKEAGRPLLVCGDLNVAPTDRDVHAKERKAN
                     AIGQRPDERALLGKVVAEGLVDVGRALDPDNDELFTYWAPWREMRKRNIGWRIDFVLA
                     STDLAKTATAARVYPGFGTSDHAPLVVDFA"
BASE COUNT          940 a         2582 c         2377 g          947 t
ORIGIN      
        1 cgcggacgcg gcccggcgcg gcgtcgcggt gcgcgtcctc gccgacgcgt tcggctcgag
       61 cgcgacccgt cgttcgttct ggcgggagct cgagggactc ggcatccgcg tgcgcctcta
      121 ccacccgctc ttcccgttcc tctggtacca cccctaccgg gaccaccgca agatcctcgc
      181 ggtggaccgc cgtgtcgcct tcacgggcgg catgaacatc ggggaggaat acggctcctc
      241 ggtccctccc gcgaagcacg gcgagacgtg gcgcgacact cacgcgcggc tcgagggccc
      301 cgtcgcatgg gagatggccg tcgtcttccg cgagggctgg gaccgggccg gcggcgcgcc
      361 gttcgagatc ccgccgctcg cgctccctcc ggacgatgcg cccacgccgc cgggcgcgtc
      421 catcctcgtc ctcgactcgg cgccggggcg cggacacgag gagacggcct cggtcctctc
      481 cgcggcgatc gcggcctcgc gcgagcgctt ctggctcacg aacgcgtact tcgccccgcg
      541 ccgcgacgcg cttcccctgc tcgcggaggc ggcccgaagg ggcgtcgacg tccgcctcct
      601 gcttcccggc gtcagcgacg tcccgctcgt gcgccacgcg ggccacgggt cctaccggca
      661 cctcctgagc cggggcgtga ggatcttcga gtaccgcacg gccgtcctgc acgcgaagtc
      721 cttcgtggcg gacgggttcc tctcgatggt cggctcgacg aacatggact tccggtcgtt
      781 tcacttcaat gccgagtgca acgtcgtcct gctcgacgcc gcgaccgcgg ggacgctcga
      841 gaagcagttc acggccgatc tcgaggacgc gacggaaatc acgctcccgg attgggaccg
      901 ccgcacgcga gcccaccggg cgggcgacgc gctggcgcgg atgctttcgc ccgtgctctg
      961 atggcctgag cggaggacga tgatggcgaa ctccccgaga acggccttcg tgccgtcccg
     1021 gaccacgatc ctcgtgctct gggtcctctc gctccttccc gccgccggcc tcgtcgggct
     1081 cgtcgtgcgg aacgacgcgc gaaaggcgga cttcctgcgc cgcgcgcggg gccgggccga
     1141 cggcaccgtg atccgcctcg tcgagcagcg tgggaaacac atccagttct atcccgtcgt
     1201 cgccttcacg accgccgacg gccggcgcct cgagttcaag tcgatctact ccgactacct
     1261 cgccttcggc gagaagcact atcccctcgg cggccgcgtc tcggtcgcct acgtcccgga
     1321 ggaccccgcg cgcgcggaga tcgacgcacc cgcgctgcac gtgaaggact ccgggctgtt
     1381 cgtcgtgggc ggcgggatct acgccggact catgacgctc gtcttcggcc tcgtggcgct
     1441 cctcacgaag aagaagagac ccggttcgag cctcccggcg gagccgctcg ctccgtcctg
     1501 aacgcgggcc ggcgctgagt atcatcggcc cgtgaaacgc cgggtcttct cgcttgcggc
     1561 cgccgccgtc ctcgcgggcg cttccgcgtc cgcgcagacc atcacgacgg tcgccggcac
     1621 gggcgacacc gccttctctc cggacggctc cccggcggcg acgtcgtcgc tcgcgctcgc
     1681 gaccgacaac gtcgccaaca tcgtcttcga ctccgacggg aacctgacgt tttccgagag
     1741 ccgcgcctgc cgcgtgcgcc ggatcgcccg aaagacgggc ctcctcgaaa ccgtcgccgg
     1801 aagcggcgcg tgcggctact cgggggacgg cggtcccgcg aaggaggccc gcctgagggc
     1861 tcccgcagag atcgcgttcg acgccaaagg caacctcttc gtcgcggacg ccgggaacaa
     1921 cctcgtgcgc cgcgtcgacc gcaagacggg agtcatcacg acggtcgcgg gcaaccggct
     1981 cccgaactac gacggcgacg gcgtcgcgac ggaggtcgcg atcgggcggc cgagcggcct
     2041 cgtgttcgac cggaaggggc gcctcgtgat ttcggaggtg tacgggtcgc ggatccgccg
     2101 tctcgacttc gccaccggga agaccgagac gatcgcggga aagaacgacc tgatgttcac
     2161 ggcgggaccc gctcgcgcga ccggcttcac gtggcccaac ggcccgcgct acgaccacag
     2221 cggagcgctc ttcttcgcgg cgtccgggaa caaccaggtg ctccggctcg acccgaaggg
     2281 cgagaccctc accgccttcg ccggcaacgg gctcgtgggc ctcaagggag acggcggccc
     2341 cgcgacggag gccgtcctca acgcgccggc cgcgctcgcc gtcgacgggg cggggaacgt
     2401 cttcatcgcg gacacgggga acaacgtggt gcggcgcgtc gacgcgaaga ccggcatcat
     2461 cacgctcgtc gccggcacgg gtgccgagga gttctcggga gacggaggcc cggcggccgc
     2521 ggcggccctg tcggacccgc tcggcctcgg cctcgacggc aagggcaacc tctacgtgat
     2581 cgacgcccgg aattaccgga tccgcaggat cgacggcgtc gcgcgctgag ccggtgaccg
     2641 tttccccccg gggccgggcg tggcaatctc cgcccatgtc gcgccgcgcc ctcgccgcct
     2701 ccctcgccgc cgcatggctc tccggttgca cggcgggtcc ttccgcctcg cccccatcgc
     2761 ccggcccggt tcccggagcc gggcgcgtcg tcgcgatctc gttcgacggc ctcggcggcg
     2821 tgcgcctgaa cgagctcctc gccgccggca cgctcgacgc gggcgggttc gcggccttcg
     2881 ccgcgcgcgg cgtcctcgcg gggcgcgccc tggacgtgac gccgtcgttg actcccgtcg
     2941 cgcacatctc cgcgatcacg ggcgcgccgc ccgcgaagac gggcatcgtc gcaaaccagt
     3001 tccgcgagcc cggctccccg ttcggcacgc agacgagcgg ctttgcggcc tcgatcggca
     3061 gcgagacgct gtgggaggcc gcgcgccggc agggtcgccg cgtgggcgtc atcacgtacc
     3121 ccggcgcgga cgccgccacc gagggccgcc gcggcgactt cggactggtc tggcccgaga
     3181 agcccgcacg ggactcggca ttcgtgagcg tcccgggcga cgcctggacc gacgcagccg
     3241 ccgcgccgaa gtcgttctcg cccccgcgcg cggcgcgggt cgccgtgcag actcccgccg
     3301 gcgcggcgac gcttcgcctc gtcgcgaccg acacgaccga cgacgcgacc gcgaactacg
     3361 acctcgtttt cgtgtcccgc gaggacggcg cggctcccac ccggctcggc gcggtcgccg
     3421 tgcacggctg gttcgcgatc tccttgccgg gcgcgcgcgg gtccttcatg gcgtggtgcc
     3481 gcctcgaaga gctcgacccg tcgctcgcgc gcgcggaact ctacgtcggc gcgttcaacg
     3541 ccgtgccggg ctacccggag gactaccgtc gccgcctcga agaggcgatc ggcggctggc
     3601 ccggcgttcc cgactacgcg ttcgtgcggc ctccccgcgg ggactatgcc gcgcacgagg
     3661 aacaggccgc gcgcctctcg gagtacctca cgaaggcgat cctcttcacg atccgcagcg
     3721 agcgcttcga ccttctcgtc ggctaccagc cgctcgtgga cgagatcgag cacgcgctcg
     3781 agcccgggcc gtacgggggc tcgaaggagg ccgtcgtccg cgcgttccgc acggccgacc
     3841 gtgccgccgc ggcgattctc gcggcgctcg gcccgcgcga ctcgttcctc ttcttcagcg
     3901 accacgggat cgtcccgctc gaggcgggcc tgaacatcga gaagtacctc ctcgaaaagg
     3961 gctggacgcc ggtcggcccc aagggcccga gggacggcga caggcgcgtg cagatctgcg
     4021 cgacgagcgg catcgcgcac gtctacctcg accccgcgct cgctcccgcc gcgcacgcgg
     4081 cggccgccgc tgcgctcctt gccgacctcc aggggctcgg ccagttgaag gacggcctcg
     4141 tcgacgaaat cgtcccccgc gccgacctcg cgcgtctcgg cctcgaccat ccgcgttcgg
     4201 gcgacgtcgt cgttctcctc aagcccggaa ccgacttctg gcgcggcggc gcacagatcc
     4261 tcggcaagcc cggcaaccgc ggagggcacg gctaccgcgc ggcctatccc atcctcgacg
     4321 cgtcgttcgg catgatcggc cccggcatcg cgcccgcccg gcccgagacc gtctccctcc
     4381 tcgaggtcgc cgcgcgcgcc gcgaatgcgc tcgggatcga ccctccccgc ggtgccgccc
     4441 ccgccgggcg ctaggatcgg ggcctgatgg agaacgcgcg cgtcgaaccg gccccgatcg
     4501 cgtgggcgag cctctcggac gtcggccgcg tccggcccgc gaacgaagac tacgtcggtg
     4561 accccgaacg gctcgccccg ctcgtcggcg gagcccggcg cctcgccgcg cgcggcgcgc
     4621 tcttcgccgt ggcggacggg atgggcggcc acgcacgcgg cgagatcgcg agcaagctgg
     4681 cgatcgacgt cctctacgcg acgttctaca actctcccgg cgatcccgtg gagatgttca
     4741 agaaggcgat cctcacggcg aacaccgccg tgcgccgcgc gggcgtgctc gcgaggcccg
     4801 agaagagcgg cgacggcgag gacgcccgga cgctgccgcg catgggcacg acgctcgtgg
     4861 ccgcgctcgt cctcgggagg tgggtcctca tcgggaacat cggggacagc cgcgcgtacc
     4921 tcatgaacga cggccgcctc gagcaggtca gccgcgacca ctcgtacctc gccgaggaga
     4981 tccggcgcgg cctcgtgccg cccgacgccg cggagacctc gccgtaccgg cacctcatca
     5041 cccgttcgct cggcacgaag gacgaggtgg aggtcgacct gttctggcgc ccgtggccgg
     5101 acgggtcgac tctgctcctc gccacggacg ggctccacgg atgcgtggag gagctcgaga
     5161 tggagacgat cctcgcgtcc cgtccgcccg acgtcgcggt gaaggagctc gtcaaggccg
     5221 ccctggaggc cggggcgccc gacaacgtct cggtcgtcgt cgtccacaag cccgtgttct
     5281 cgcggaagaa ggagcgcgcg tgaagctctt cgcgggcacg agcgggttca gctacgacgg
     5341 gtggacgggt tcgttctacc ccgaggacac gcccgccgac gggcgcctcg cgtactacgc
     5401 ggcgaagctt cccgcggtcg agatcaacaa cacgttctac cggatgccca agcccgagct
     5461 cctcgcgagc tgggccgccc aggttcccgc ggacttccgg ttcgcgctca aggcctcgca
     5521 gcgcatcacg cacatcaagc gcctcaagga ggtcgacggt gaggtcaagt tcctcctcgg
     5581 cgccgcgcgc cagctcggcg acaagctcgg tccgatcctc tacggcctgc cgccgaatct
     5641 caagaaggac gtcccgcgcc tcgcggcgtt cctcgcactc ctgccgtccg acgcgaaggc
     5701 cgcgttcgaa ttccgcaacc cgacgtggct cgacgcggag gtctacgcgc tcctgaagtc
     5761 ggccggcgcg gcgctctgcg tcgccgacga cgaaaagggc acgacgccgc tcgaggcgac
     5821 ggcgccgtgg gggtacgtcc ggctgcggcg cgaggattac ggggagaagg acctcgcggc
     5881 atgggcggag cgcgtgcgcg ccgccggctg gaccgaggcg tacgtcttct tcaagcacga
     5941 ggaggagggc gccgccccgg ccctcgcggc ccgcttcgcg gaggtcgccg gatgaaggtc
     6001 gccacctgga acgtcaacgg gatccgcgcg cgcgagctgc aggtcctgga attcctcgac
     6061 acgcacgcgc cggacgtcgt ctgcctgcag gagttgaagg ccgcgcccga gcagatgccg
     6121 atctcgctcg cggcgcccgc gggctacgcg tcctactggc acggccacaa gggatactcg
     6181 ggcgtcgggc tcctcctgaa gaccgcgacg ttcggcgaga cggccggctt ctcgcatccc
     6241 gacttcgacc acgagacgcg gatcgtcgag gcggcgacgc cgaagttctc cgtggcgtcc
     6301 gtctacgtcc cgaacggcgg caaggactac gccgcgaagc tcgcgttcct cgaggcgatg
     6361 gcggactggg cgcacgcggc gaaggaggcg ggccggccgc tcctcgtctg cggcgacctg
     6421 aacgtcgcgc ccacggaccg ggacgtccac gcgaaagagc gcaaggcgaa cgcgatcggc
     6481 cagaggccgg acgagcgggc gctcctcggg aaggtcgtcg ccgagggtct cgtggacgtc
     6541 ggccgcgcgc tcgaccccga caacgacgag ctgttcacgt actgggcccc gtggcgcgag
     6601 atgcgcaagc gcaacatcgg ctggcggatc gacttcgtcc tcgcgagcac ggacctcgcg
     6661 aagacggcga cggccgcgcg cgtgtacccc gggttcggca ccagcgacca cgccccgctc
     6721 gtcgtcgact ttgcctgagc ccggctagac tgggacatgg cgccgccgcg ggtgagactc
     6781 acgacgagtc ccggctggga ggtcgtgcgc gaccggcccc ggcgagccgc cgcccgggcc
     6841 gccgcg
//
LOCUS       DAOMFY010000217         6806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3651_length_6806_cov_7.683602, whole genome shotgun sequence.
ACCESSION   DAOMFY010000217 DAOMFY010000000
VERSION     DAOMFY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3651_length_6806_cov_7.683602"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLB01_14850"
     CDS             complement(<1..218)
                     /locus_tag="PLB01_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78632.1"
                     /translation="MSAKEDLPFRVRPPRPGEWPKTAADAALLATDPDGALVAADDDG
                     RIFGRAAAAVREDALLLARLEVDPAARGG"
     gene            283..903
                     /locus_tag="PLB01_14855"
     CDS             283..903
                     /locus_tag="PLB01_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ78633.1"
                     /translation="MGIVQRREREKLEVRTKILDAARELFAREGYDAVTMRRIADAIE
                     YSPTAIYLHFKDKETLIRDLCEEDFASLAKAFQRIAREPDPLERIRKAGMAYVDFGSD
                     RPNHYRLMFMTPRAHEEMRNLEKGNPEEDAYAFLERAVAEAIAKGLLRPELKDPHLVA
                     QAAWAGVHGVISLHLAKKDDPWVEWRPLKKTAALVVDSMIEGFRKG"
     gene            907..2034
                     /locus_tag="PLB01_14860"
     CDS             907..2034
                     /locus_tag="PLB01_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78634.1"
                     /translation="MVSLARKNLLHDRLRFVITISGVAFAVTLVFVQVGLFLGLLDNA
                     SVTIEKMSADLWVTSKNTPNVDFAHTFPETAVQRVRSVPGVARADNLIVWFMQMSLPS
                     GAQEGTLVYALEDFTAWNIPWNVAEGDLSDLKRGNYFFLDSSALRRFGPFAVGEYREV
                     LDRRLKIIGRTENARSFTTTPISFMDLRLAQELSYQTMHDKTTYVVVKLAPGADLESV
                     RAEIRRRLPFNDVFTRAEWAKRSRAYWVANTGIGLNMYLTVFLGVLVGIVVVAQTLYT
                     ATMEHIKEFGTVKAIGGGNRDIYAILAEQAAISAVVGFVCGLLPAFALKPLLAKADLK
                     IIVTPEFAVIVFVGTLAMCLLSAMVSFRKVAAIDPALVFRG"
     gene            2043..2717
                     /locus_tag="PLB01_14865"
     CDS             2043..2717
                     /locus_tag="PLB01_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78635.1"
                     /translation="MSVLVSENVVKRFTEGRESVEVLKGVSLSVGRGEVVSLEGPSGS
                     GKTTFLSILGCILTPTEGRVAIDGEAVDPKRPARLPEIRKKSIGFVFQQFNLFPALTA
                     LENVEYALNVKGLKGGEAAREAHRVLDAVGLSDRKRFLPRDLSGGQKQRVAIARALAG
                     SPSVLLADEPTANLDSHVGLQILELFRDLAKKENKALVVVTHDPKVRSIADRVVTIRD
                     GVLAAA"
     gene            2743..3687
                     /locus_tag="PLB01_14870"
     CDS             2743..3687
                     /locus_tag="PLB01_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ78636.1"
                     /translation="MSNQEKDLHVVFGTGPLGRAVAAALVARGRAVRAVGRSARADVP
                     AGTAYVSADASEPAAARAVTLGAAAVYQCAQPAYHRWPEEFPRLQSSILEAAAAAGAR
                     LVLADNLYALGDAGRPLTEDLPAAATTRKGRTRGAMAEAALAAHRAGRVRVAIGRASD
                     FYGPGVRLSALGERVFAPLLAGKKAGVLGNPDLPHTYTYIDDFARALVTLGERDEASG
                     GIWNVPSAPTLTTREIVAIAAEEAGVEPRLAAAGRALLSLAGLFNPGAREMLEMFYEF
                     EKPFVVDHSKFGRAFGGAATPHRDALQATLAWYRARKA"
     gene            3708..4613
                     /locus_tag="PLB01_14875"
     CDS             3708..4613
                     /locus_tag="PLB01_14875"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ78637.1"
                     /translation="MKRPPLALSIAVAASGTAVLLLCRSAAQGGGAATPAPKPLAVAS
                     APAARVAAEGRVVAYPGAEVVVGTDFAGTVRRLLVKERDAVRKGDLLAEIGADEQKAA
                     LAEAEARIAEAAADVKLFEFELARAEQLLASKVGARQAVDKASRDLEAARARRATAGA
                     EAERLKATIAKSRILAPISGAVITRHVEAGETIDRGARIATVADLSRLRVEAEVDEAD
                     AARVSVGARVAIRAEGESGAGLEGRVEEIPDAVTGRRTKPLDPGKPSDTRVLLVKIAF
                     ADASRGTALKLGRRVEVEIQGGSPR"
     gene            4669..5265
                     /locus_tag="PLB01_14880"
     CDS             4669..5265
                     /locus_tag="PLB01_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ78638.1"
                     /translation="MKKRTVGRPRDGDPAKTRREILEAAEESFAASGFVGATTRQVAA
                     RAGVNVATLHYHFGNKERLYRAVLEAAVAGDVPDGPTAGSPAERLTGVVEALWSFGLK
                     RSALSRLSLLHRLAGPARTAGRAPEPDDPRETLLAKTIADVAEKGSLPPEEAARLVLV
                     LMDGALVAVRNGHGPDAAVPEAPRRAVVAAALRITGLA"
     gene            complement(5262..6587)
                     /locus_tag="PLB01_14885"
     CDS             complement(5262..6587)
                     /locus_tag="PLB01_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78639.1"
                     /translation="MNLALALVVYGAAVLLVGVVSARKASRSTDAFLVADRGLGGLRA
                     GVALSSTVIGGSTTLVLAALVASKGLPALWLDLAGALGLLALGAFLAPRLRATGATTI
                     AEVIGRTYGGGVRRIAACLVVSAEIVWFALLAQATQTVIVATTPWDPRRVLILTAALF
                     VAYTALGGQRAVVGTDLLQFALMVCGLLFVALPLAFAALLRTPPPGRLLAFPTGPAFS
                     WADVLAFLVLVGLPHAVGSDVWSKLLSARDASAARRAAYGAAVAKLAFGLSTCAIALA
                     GVSLAVPGPPAQLFPRTVFALAGPALAPLLLVALVATMQSSADSVLLSAAAATAHDLV
                     PGAGVRFARAAVVVYGAAGLAVALVLQDLVETFRLGYTLFASGLILPTLAAFVPGVRV
                     DRRFAGSAMLLGGAAAMLERFLHVAGVDPVLAGTGVNAIVLACGVRRSP"
     gene            complement(6584..>6806)
                     /locus_tag="PLB01_14890"
     CDS             complement(6584..>6806)
                     /locus_tag="PLB01_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78640.1"
                     /translation="GRASAECRTMGGEDFSEFLQRVPGAFAFVGAARTDGPRGPHHAP
                     DFDFDERALPHAARLLAAYARDVLTAPPA"
BASE COUNT         1058 a         2387 c         2455 g          906 t
ORIGIN      
        1 cctccccgcg ccgcggggtc gacctcgagg cgcgcgagaa ggagcgcgtc ctcgcgcaca
       61 gcggccgcgg cccgcccgaa gatccgcccg tcgtcgtcgg cggccacgag agcgccgtcg
      121 gggtccgtcg cgaggagcgc ggcgtccgcc gcggttttcg gccactcgcc cgggcgcggg
      181 ggccggaccc ggaacggaag gtcttctttc gcgctcaccc ttgactgacg ctaacacggg
      241 tcctatactt aacattgtta agtaaatgag ggagattcag gcatgggcat cgtccagcgg
      301 cgggagcggg aaaaactgga ggtcaggacc aagatcctcg acgcagcgcg cgagctcttc
      361 gcccgcgagg gatacgacgc cgtcacgatg cgccgcatcg ccgacgcgat cgaatactcg
      421 ccgacggcca tctacctgca tttcaaggac aaggagacgc tcatccgcga cctctgcgag
      481 gaggacttcg cctcgctcgc gaaggcgttc cagaggatcg cgcgggagcc ggaccccctc
      541 gagcgcatcc gcaaggccgg gatggcgtac gtggacttcg gcagcgacag gccgaaccac
      601 taccggctca tgttcatgac gccgcgggcg cacgaggaga tgcggaacct cgagaaggga
      661 aatcccgaag aggacgcgta cgcgtttctc gagcgcgccg tcgccgaggc gatcgcgaag
      721 ggccttctga gacccgagct gaaggacccc cacctcgtcg cgcaggcggc ctgggccggc
      781 gtccacggcg tgatctccct ccacctcgcc aagaaggacg atccgtgggt cgagtggagg
      841 cccctgaaga agaccgcggc gctcgtcgtc gattcgatga tcgaaggctt ccggaaaggc
      901 tgagccgtgg tctccctcgc gcggaagaac ctcctccatg acaggctgcg cttcgtgatc
      961 acgatctcgg gcgtcgcgtt cgcggtcacg ctcgtcttcg tccaggtcgg cctcttcctg
     1021 ggcctcctcg acaacgcgtc cgtcacgatc gagaagatgt cggcggacct ctgggtcacg
     1081 tcgaagaaca cgccgaacgt ggacttcgcc cacacgttcc cggagacggc ggtccagcgc
     1141 gtgcgctcgg tgccgggcgt cgcgcgggcg gacaacctga tcgtctggtt catgcagatg
     1201 tcgctcccct cgggagcgca ggaggggact ctcgtttacg cgctcgagga cttcacggcc
     1261 tggaacattc cgtggaacgt cgccgagggc gacctctcgg atctcaagcg cggcaattac
     1321 ttctttctcg attcgtcggc gctccgccgt ttcgggccgt ttgcggtcgg cgagtaccgg
     1381 gaggttctcg accggcgcct caagatcatc gggcggacgg agaacgcacg ctcgttcacg
     1441 acgacgccga tctcgttcat ggacctccgc ctcgcgcagg agctctcgta ccagacgatg
     1501 cacgacaaga cgacgtacgt cgtcgtgaaa cttgcgccgg gggcggatct cgagtccgtg
     1561 cgcgccgaaa tccggcgccg gctcccgttc aacgacgttt tcacaagggc ggagtgggcg
     1621 aagcgctcgc gcgcctactg ggtcgcgaac acgggcatcg gcctgaacat gtacctgacg
     1681 gtcttcctcg gcgtcctcgt gggcatcgtc gtcgtcgcgc agacgctcta cacggcgacg
     1741 atggaacaca tcaaggagtt cgggaccgtc aaggcgatcg gcggcgggaa ccgcgacatc
     1801 tacgccatcc tcgccgaaca ggccgcgatc tcggccgtcg tcggcttcgt ctgcgggctc
     1861 ttgcccgcct tcgcgctcaa gccgcttctc gcgaaagccg acctcaagat catcgtgacg
     1921 cccgagttcg cggtgatcgt gttcgtcggg acgctcgcga tgtgcctgct ctcggcgatg
     1981 gtctcgttcc ggaaagtcgc cgcgatcgat ccggcgctcg tcttccgcgg ttaggagtcc
     2041 tcatgtccgt tctcgtgtcc gaaaacgtcg tcaagcgctt caccgaaggc agggagtccg
     2101 tcgaggtcct gaagggcgtc tcgctctccg tggggcgcgg agaggtcgtg tcgctggagg
     2161 gtccgtccgg ctcggggaag acgacgttcc tctcgattct cgggtgcatc ctgacgccga
     2221 ccgaaggccg cgtcgcgatc gacggagagg ccgtggaccc gaagcggccc gcgcgcctcc
     2281 cggagatccg gaagaagtcg atcggtttcg tcttccagca gttcaacctc ttcccggcgc
     2341 tcaccgctct cgaaaacgtg gagtacgcgc tcaacgtgaa gggcctgaag ggcggcgagg
     2401 ccgcgcgcga ggcgcaccgc gtcctcgacg ccgtcggcct ctcggaccgg aagcgcttcc
     2461 tcccgcgcga cctctcgggc ggccagaaac agcgcgtcgc gatcgcgcgc gccctcgcgg
     2521 gctcgccgtc ggtcctcctc gccgacgagc cgaccgcgaa cctcgactcg cacgtggggc
     2581 tccagatcct cgagctcttc cgcgatctcg cgaagaagga gaacaaggcc ctcgtcgtcg
     2641 tgacgcacga cccgaaggtc cgttcgatcg ccgaccgcgt cgtcacgatc cgcgacggcg
     2701 tgctcgccgc cgcctgaccc accggggaca aaggaccacg acatgtcgaa ccaggagaaa
     2761 gatctccacg tcgtcttcgg gaccggcccg ctgggccgcg cggtcgccgc ggctctcgtc
     2821 gccaggggac gggcggtccg cgccgtcggc cgctcggcgc gagccgacgt tccggcaggg
     2881 accgcgtacg tctctgccga tgcttccgaa ccggccgcgg cgcgcgccgt caccctcggg
     2941 gctgccgccg tctaccagtg cgcccagccc gcctaccacc gctggccgga ggagtttccc
     3001 cggctgcagt cctccattct cgaagcggcc gcggccgccg gggctcgcct cgtcctcgcc
     3061 gacaacctgt acgccttggg cgacgcgggg cggccgctga cggaggatct gcccgccgcg
     3121 gcgacgacgc gcaaggggag gacacggggg gcgatggccg aggctgcgct cgcggcccac
     3181 cgcgccggcc gggtccgggt cgccatcgga cgcgcttccg acttctacgg gcccggcgtc
     3241 aggctgtcgg ccctcggcga gcgcgtcttc gcgccgctgc tcgcgggcaa gaaggcggga
     3301 gttctcggca accccgacct gccgcacacc tacacgtaca tcgacgactt cgcccgagcc
     3361 ctcgtgactc tcggggagcg cgacgaggcg tccggtggga tctggaacgt gccgagcgcg
     3421 ccgacactga cgacgcgcga gatcgtcgcg atcgcggcgg aagaggcggg ggtcgagccg
     3481 cgcctcgcgg cggccggacg ggcactcctc tcactcgccg gtctcttcaa tccgggcgcg
     3541 cgggagatgc tggaaatgtt ctacgagttc gagaagccgt tcgtcgtgga tcactcgaaa
     3601 ttcggccgtg cctttggcgg cgcggcgaca ccgcaccgcg acgcgctcca ggccactctg
     3661 gcctggtacc gcgcgcgcaa agcctgaatt ccgccggagg actgccgatg aaacgcccgc
     3721 cgctcgccct ttcgatcgcc gtcgccgcct cgggaacggc ggttctgctc ctctgccggt
     3781 ccgccgcgca ggggggcggc gccgcgacgc ccgccccgaa gccgctcgcc gtcgcgtccg
     3841 cccccgccgc gcgcgttgcc gccgaggggc gagtcgtcgc gtacccgggc gccgaggtcg
     3901 tcgtcgggac cgacttcgcc ggcacggtcc ggcgcctgct cgtgaaggag cgggacgccg
     3961 tccggaaggg cgacctcctc gcggagatcg gcgccgacga acagaaggcc gccctcgccg
     4021 aggccgaggc gaggatcgcg gaggctgccg cggacgtgaa gctcttcgag ttcgagctcg
     4081 cccgcgccga acaactcctc gcgtcgaagg taggcgcccg acaggccgtc gacaaagcct
     4141 cccgcgacct cgaggcggca cgcgcgcgcc gcgcgacggc cggtgctgag gccgagcggc
     4201 tgaaggcgac gatcgccaag tcccggatcc tcgcgccgat ctccggcgcc gtgatcaccc
     4261 gccacgtcga ggcgggcgag acgatcgacc gcggagcacg catcgcgacg gtcgcggacc
     4321 tctcgcgcct gcgcgtggag gcggaggtcg acgaagccga cgccgcgcgc gtttcggtcg
     4381 gagctcgtgt ggcgattcgc gccgaggggg agtctggcgc ggggctggaa ggccgcgtcg
     4441 aagagatccc ggacgccgtc acgggccgcc ggacgaagcc tctcgatccg ggaaagccct
     4501 cggatacgcg cgtcctcctc gtgaagatcg cttttgccga cgcgagccgg ggaacggccc
     4561 tcaagctggg ccgtcgggtc gaggtggaga tccagggcgg ctcgccccgc tgaccaatca
     4621 cccggtttgt ttagaatcca aattcaccac ggggacggat tgatcgggat gaagaagcgc
     4681 accgtgggcc gtccacgcga cggcgatccc gccaagacgc gccgcgagat cctcgaagcg
     4741 gccgaggaaa gttttgcagc gtctggattt gtcggcgcga cgacccggca ggtggccgcg
     4801 cgcgccggcg tcaacgtggc gaccctccac tatcacttcg ggaacaagga gcgtctctat
     4861 cgcgccgtcc tcgaggccgc ggttgccggc gatgttcccg acggtccgac ggcgggctcg
     4921 cccgcggagc gcctgaccgg cgtcgtcgaa gcgctctgga gcttcggcct gaagcgctcc
     4981 gcgctgtccc gcctcagcct cctccaccgc ctggccggac ccgcgcgcac cgcgggacgc
     5041 gcgcccgaac cggacgaccc gcgcgagacg ctcctcgcga agacgatcgc ggacgtcgcc
     5101 gagaaggggt ctctcccgcc cgaggaggct gcgcgtctcg tactcgtcct gatggatggc
     5161 gcgctcgtgg cggtgcgcaa cgggcacggc ccggacgccg ccgttcccga ggccccccgt
     5221 cgcgccgtcg tcgcggcggc gctccgcatc accgggctgg cctagggact tcggcgcacg
     5281 ccgcacgcga ggacgatcgc gttgacgccg gttcccgcga ggaccgggtc cacgccggcg
     5341 acgtgaagga aacgctcgag catcgccgcg gccccgccga ggagcatggc ggaaccggcg
     5401 aagcgccggt cgacgcggac gccggggacg aacgccgcga gcgtcgggag gatgaggccc
     5461 gacgcgaaga gcgtgtagcc gagccggaac gtctcgacga ggtcctggag gacgagcgcg
     5521 acggcgagcc cggccgcgcc gtagacgacg acggcggccc gcgcgaagcg cacgccggcg
     5581 ccggggacaa ggtcgtgcgc cgtcgccgcg gcggccgaga ggagtaccga gtccgccgag
     5641 gactgcatcg tggcgacgag cgcgacgagg aggagcggag cgagcgcggg gcccgcgagc
     5701 gcgaagaccg tgcgcgggaa gagctgggcc ggcgggcccg ggacggcgag cgagacgcct
     5761 gcgagagcga tcgcgcacgt cgagaggccg aacgcgagct tggcgacggc cgcgccatac
     5821 gcggcgcgcc gcgccgcgga ggcgtcgcgc gcggagagga gcttggacca tacgtcgctg
     5881 ccgaccgcgt gcggcagccc gacgagaacg aggaacgcga ggacgtcggc ccacgagaac
     5941 gcaggcccgg tggggaatgc gagaagccgt ccgggcggcg gcgtccggag gagcgcggcg
     6001 aacgcgagcg gaagcgcgac gaagaggagg ccgcagacca tgagcgcgaa ctggaggagg
     6061 tccgtcccga cgacggcgcg ctggcccccg agcgccgtgt aggcgacgaa gagcgcggcc
     6121 gtcaggatga ggacgcgccg cggatcccag ggcgtcgtcg cgacgatcac ggtctgcgtc
     6181 gcctgcgcga ggagcgcgaa ccagacgatc tcggccgaga cgacgaggca cgccgcgatg
     6241 cgcctcacgc cgcctccgta cgtcctcccg atgacctccg cgatcgtcgt cgcgccggtc
     6301 gcccggaggc gcggggcgag aaacgcgccg agcgcgagga ggccgagcgc gcccgcgagg
     6361 tcgagccaga gcgcggggag acccttcgac gccacgagcg cggcgaggac gagcgtcgtc
     6421 gagccgccga tcaccgtcga ggagagcgcg acgcccgccc ggagcccgcc gagcccgcgg
     6481 tccgcgacga ggaacgcgtc cgtggagcgc gaggccttcc gggccgagac gacgccgacg
     6541 agcaggaccg cggcgccgta gacgacgagc gcgagcgcga gattcacgcg ggcggggccg
     6601 tgaggacgtc gcgggcgtac gcggcgagga ggcgggccgc gtgcgggagg gcgcgttcgt
     6661 cgaagtcgaa atccggcgca tggtgcggcc cgcgggggcc gtccgtgcgc gcggcgccga
     6721 cgaacgcgaa ggcgccgggg acgcgctgga ggaactcgga gaagtcctcc ccgcccatcg
     6781 tccggcactc ggccgaggcg cggccc
//
LOCUS       DAOMFY010000218         6746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3702_length_6746_cov_6.667015, whole genome shotgun sequence.
ACCESSION   DAOMFY010000218 DAOMFY010000000
VERSION     DAOMFY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3702_length_6746_cov_6.667015"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLB01_14895"
     CDS             complement(<1..359)
                     /locus_tag="PLB01_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78641.1"
                     /translation="MMEIRDDQRAVEALTPEEMERDRRIELLVDECVRSSPAPGPEFT
                     AAILAARPFAPWEVRKARAWRVPALAASGLLAASLAVFLAPLWSLGPAAAMELWARVV
                     VASASGALGAALAAAPAL"
     gene            complement(369..950)
                     /locus_tag="PLB01_14900"
     CDS             complement(369..950)
                     /locus_tag="PLB01_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ78642.1"
                     /translation="MKAAPERDDAELVRAALESDADAFGILVERYRVRLYRFVGRYTN
                     DAEDARDVTQEVFLKVHAALDSYDPKYKFSTWLFRIAGNAAIDHLRRRKVRPLPLEIP
                     VGEDGESRVAEPRESRPDPLEDLTRRRLREALAAAIDRLPDDYRELISLRHYGEMPYE
                     EIAELKGMPLGTVKNKLFRARQALRDLLPKDIV"
     gene            complement(947..3712)
                     /locus_tag="PLB01_14905"
     CDS             complement(947..3712)
                     /locus_tag="PLB01_14905"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HQQ78643.1"
                     /translation="MTTPGDHLLEALQERAKELSTIYRVNEACRAPQSSLDEVFRNVV
                     RILPAGWQFPEACVARLTVDGTVYGPPGAVASPWRQAATIRVQGEPAGTLEVFYEEER
                     PAADEGPFLKEERRLLDAVAERLGQLLLERRLLDALHGRREDDASDAPDAEWRVIVDF
                     LRQTDPRHLIRLSRRMINYLCWNGVAAAQDLLPHFAGGREPLESDENRPSPRRSAEHL
                     LGVADAAFRLASLHLSSAEIRSCIQKWIRDDKADFLLRAAENPGTSTAELAQALARFH
                     DSHLDDRQLSRTVQVELRVSLARRFLTDDLGFVDVAKDFIDTADFFDLVRHVISPPRS
                     YGRVGGKASGLFLASQIVKKSTEFADALGEIRIPKTWYVSSDAILDFIEFNQLDDLYD
                     RKYLEIDQVRREYPHVIQVFKNSHFSPEIVKGLSVALDDFGDRPLIVRSSSLLEDRVG
                     AAFSGKYKSLFLANQGPKAERLTALLDAIAEVYASVFGPDPLEYRAERGLLDVHEEMG
                     ILLQEVVGSRVGRYFLPTFAGVAFSNNEFRWSPRIAREDGLVRLVAGLGTRAVDRVAD
                     DYPILVAPGKPGLRVNVTPEEVVRYAPRKVDVIDLQSGTFETLDLERLLDECGADIPG
                     VEQVLSVSDETGVHRPYFLDWTSKKGRFIATFETLLESTPFLSRMRALLRVLREKTHG
                     PVDIEFASDGKDLYLLQCRPQSFAEESAASVIPSDLSGDRVIFEAKRFVSNGRVPDIT
                     HVVYVDPEGYAALPDAASLRRVGQAVGRMNKLLPKREFILMGPGRWGSRGDLRLGVPV
                     GYSDIHNAAALIEIARRRGGYVPDLSFGTHFFQDLVEASIRYLPLFPDDPDVRFREAF
                     LKDSPSVLLELAPEFRDLEAVVRVIDVPKVTGGFVLKLLLNADEDHAVAILSAPGPGA
                     RTRVSRS"
     gene            complement(3755..5038)
                     /locus_tag="PLB01_14910"
     CDS             complement(3755..5038)
                     /locus_tag="PLB01_14910"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQQ78644.1"
                     /translation="MTGSFNAFEMAQTQFDKSADILALDAPTRDLLRWPLREYWFAIP
                     VRMDDGKVKVFRGFRVQHNDARGPSKGGIRFHPQETIDTVRALSMWMTWKCAVVDIPL
                     GGAKGGVICDPHNLSAREQEGICRGWVRQVAKNVGPAIDVPAPDVMTTPQHMLWMLDE
                     WETIHGHRGPGFITGKPVGMGGSLGRTEATGYGAVFTLREALRKMGIDVAKTKASVQG
                     FGNVSQYAIQLYTKMGGTVVAVSSWDQADQTSYTFRKESGVDGAQLMGITDHFGGIDK
                     AKAKALGYEVKPGGDWIAEDVDILIPAAIENQVTGETAKKIAKRVKVVVEGANGPTTP
                     EADAILKERGIFVIPDFLANAGGVTCSYFEQVQSNMNHYWTQEEVLKKLDDKMTRAFH
                     DVADLAASRKLYMRDAAYVIAVSRVAKACRDRGWV"
     gene            5215..5949
                     /locus_tag="PLB01_14915"
     CDS             5215..5949
                     /locus_tag="PLB01_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78645.1"
                     /translation="MIRFERAVKRYGDRTALSDFTLEIPAGALFALVGPNGAGKTTAI
                     GLLTGLLSPTSGRTFLAGIDVARDPVAAKAKLGFVPDRPYLWPKWTPRETLRFVGAVF
                     GARGAALESRIDAELDAFDLVAVADARSDTLSHGTRQRVALAQAFLHDPEVLVLDEPM
                     VGLDPIAQRSLGERLRTLAARGSAVLLTTHQLAQAEELATDAGLLFDGRLLARGAPGA
                     LRTASGVTGALSEVFFALAGEGDKPA"
     gene            5946..>6746
                     /locus_tag="PLB01_14920"
     CDS             5946..>6746
                     /locus_tag="PLB01_14920"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78646.1"
                     /translation="MNAVYRANAARAAARSLLGARGRARIPALAGLFSLALFVALEVV
                     GLEAAVRASSAALAFLPELRPLFLVERLLAGAFGAAAALVLLGSLTTAVSTLFLSEEL
                     LALAVLPIPHRRLLARQAALTLALASAPSLLLALPALGVAASASHAPLLAAAAGLLSV
                     AGLFLLAGSVGIAGALVLVRIVPARRARLFSALLSALGLSAALVGFRAARPERLLDPV
                     EALGLLQSLGASAPPAPGASPAGWAARSAALALAGDPAGLLPGALLLAL"
BASE COUNT          939 a         2402 c         2357 g         1048 t
ORIGIN      
        1 agggccggag cggcggccag cgcggcgccg agcgcccccg aggccgacgc gacgaccacc
       61 cgggcccaca actccatcgc ggcggcgggg ccgagcgacc acagaggggc cagaaacacg
      121 gccaggctgg ccgccaggag gccggacgcg gccagtgccg gaacgcgcca ggcccgggcc
      181 ttccggacct cccacggggc gaacggccgg gctgccagga tggcagccgt gaactcgggc
      241 ccgggggccg gcgacgatct aacacactca tccacaagga gttcgatgcg gcggtcccgc
      301 tccatctcct caggggtgag ggcctcgacg gcccgctgat cgtcccgaat ctccatcatc
      361 ccgcctctct atacgatgtc cttcggcaaa agatctcgaa gagcctgcct cgcccggaag
      421 agcttgttct tgaccgttcc gagcggcatg cccttgagct cggcgatctc ctcgtacggc
      481 atctcgccgt agtgccggag gctgatgagc tcgcggtagt cgtccggcag gcggtcgatc
      541 gccgcggcga gggcctcgcg caggcgtctg cgcgtgaggt cctcgagagg gtcgggacgg
      601 ctctcgcgcg gctcggcgac gcgcgactct ccgtcctctc ccaccgggat ctcgagcggg
      661 agcggccgga ccttgcggcg cctcaggtgg tcgatcgcgg cgttgccggc gatccggaag
      721 agccaggtcg agaacttgta cttcgggtcg tacgaatcca gggccgcgtg gaccttcagg
      781 aagacctcct gcgtgacgtc ccgggcgtcc tcggcgtcgt tcgtgtaccg cccgacgaag
      841 cggtagagcc ggacgcggta gcgttccacg aggattccga acgcgtcggc gtcgctctcg
      901 agggcggcgc ggacgagctc ggcgtcgtcg cgttccggcg cggccttcac gaacgggaga
      961 cccgcgtccg ggcgcccggc ccgggcgccg agaggatcgc gacggcgtgg tcctcgtccg
     1021 cgttcaggag cagcttcagg acgaacccgc ccgtgacctt cggaacgtcg atcacgcgga
     1081 cgaccgcctc gaggtcgcgg aactccggcg cgagctcgag gaggacgctc ggcgagtcct
     1141 tgaggaaggc ctcgcggaac cgaacgtcgg gatcgtccgg gaagagcggc aggtagcgga
     1201 tggacgcttc gacgaggtcc tggaagaagt gcgtcccgaa cgagagatcg gggacgtaac
     1261 ccccgcgccg gcgcgcgatc tcgatgagcg cggcggcgtt gtggatgtcc gagtacccga
     1321 cggggacccc gagccggagg tcgccgcggc tcccccaccg ccccggtccc atgaggatga
     1381 attcgcgctt cggaagcagc ttgttcatgc gtcccacggc ctgacccacg cgccggagcg
     1441 aggccgcgtc gggcagagcc gcgtagcctt cgggatcgac gtagacgacg tgggtaatgt
     1501 ccggcacgcg gccgttcgag acgaaccgct tcgcctcgaa gatcacgcga tcgccggaga
     1561 ggtcggaggg gatcacggag gccgcggact cctccgcgaa gctctgcggc cgacactgca
     1621 ggaggtagag gtccttcccg tcggacgcga attcgatgtc gacggggcca tgcgtcttct
     1681 cgcgcaggac gcggagcagg gcgcgcatgc gggagaggaa cggcgtcgac tcgagaagag
     1741 tctcgaacgt cgcgatgaac cggcccttct tcgacgtcca gtccaggaag tacggccggt
     1801 ggacaccggt ctcgtcggac acggagagga cctgctcgac gcccggaatg tccgctccgc
     1861 actcgtcgag gaggcgttcg agatccagcg tctcgaacgt cccggactga aggtcgatga
     1921 cgtccacctt ccggggcgcg tagcggacga cctcctcggg cgtgacgttc acgcgcaggc
     1981 cgggcttccc gggcgcgacg aggatcgggt agtcgtccgc gacgcggtcc acggcgcggg
     2041 tcccgagccc cgccacgagc cgcacgagcc cgtcctcgcg cgcgatccgc ggcgaccacc
     2101 ggaactcgtt gttcgagaac gcgacgcccg cgaacgtcgg aaggaagtag cgcccgacgc
     2161 gactcccgac gacctcctgc agcaggatgc ccatctcctc gtggacgtcg aggagcccgc
     2221 gctcggcgcg gtactcgagg gggtcgggac cgaagaccga ggcgtacacc tcggcgatcg
     2281 cgtcgagaag cgccgtgaga cgctcggcct tcgggccctg gttcgcgagg aagaggctct
     2341 tgtacttgcc cgagaaggcg gcccccacgc ggtcctcgag aaggctcgag ctgcgcacga
     2401 tgagcggccg gtcgccgaag tcgtcgaggg cgaccgagag gcccttgacg atctcggggg
     2461 agaagtggga gttcttgaag acctggatga cgtgcgggta ctcgcgccgg acctggtcga
     2521 tctcgaggta cttgcggtcg tagaggtcgt cgagctggtt gaactcgatg aagtcgagga
     2581 tcgcgtccga cgagacgtac caggtcttcg ggatccggat ctcgccgagc gcgtccgcga
     2641 actcggtcga cttcttgacg atctgggacg caaggaagag gccggaggcc ttcccgccca
     2701 cgcggccgta gcttctcggc ggcgagatca cgtggcgcac gaggtcgaag aagtccgccg
     2761 tgtcgatgaa gtccttcgcg acgtccacga acccgaggtc gtcggtcagg aaccggcgcg
     2821 cgagcgacac gcggagctcg acctggaccg tgcgcgaaag ctggcggtcg tcgagatggg
     2881 agtcgtggaa gcgggcgagc gcctgcgcga gctccgcggt ggacgtgccc gggttctccg
     2941 cggcgcggag aaggaagtcg gctttgtcgt cccggatcca cttctggatg cacgagcgga
     3001 tctccgcgga cgagaggtgc aaggacgcga ggcggaacgc ggcgtcggcg acgccgagca
     3061 ggtgctcggc gctccggcgc ggcgagggcc ggttctcgtc ggactcgagc ggctcgcgcc
     3121 cgcccgcgaa gtgagggagg aggtcctgcg cggccgcgac gccgttccag caaaggtaat
     3181 tgatcatccg gcgcgagagc cggatcaggt ggcgcggatc cgtctggcga aggaagtcga
     3241 cgatgacccg ccactcggcg tccggagcgt ccgaggcgtc gtcttcgcgg cgcccgtgga
     3301 gcgcgtcgag aaggcgccgc tcgaggagga gctgcccgag ccgctcggcg accgcgtcga
     3361 ggagacggcg ctcttccttg aggaacggcc cctcgtccgc cgccgggcgc tcctcctcgt
     3421 agaagacctc gagcgtgccc gcgggttcgc cctggacgcg tatcgtcgcg gcctgccgcc
     3481 acggagaggc gaccgctccc ggcgggccgt agaccgttcc gtccaccgtg agacgcgcga
     3541 cgcacgcctc gggaaactgc cacccggccg ggaggatccg gacgacgttc cggaacacct
     3601 cgtccagcga ggactgcggc gcccggcacg cctcgttgac gcggtaaatc gtgctgagct
     3661 ctttggcccg ctcctggagc gcctcgagga gatgatcgcc cggcgtcgtc acgtcaggcc
     3721 ccagtttacc gggacgacgc tggacggaag gaccctagac ccagccgcgg tcgcggcagg
     3781 ccttcgcgac cctgctgacc gcgatcacgt acgcggcgtc ccgcatgtag agcttgcgcg
     3841 acgccgcaag gtccgcgacg tcgtgaaacg cgcgcgtcat cttgtcgtcg agcttcttga
     3901 ggacctcttc ctgcgtccag tagtggttca tgttgctctg gacctgctcg aagtagctgc
     3961 acgtgacgcc gccggcgttc gcgaggaagt ccgggatcac gaagatgccg cgttccttga
     4021 ggatcgcgtc cgcctcgggc gtcgtcgggc cgttcgcgcc ctcgacgacg accttcacgc
     4081 gcttcgcgat cttcttcgcg gtctcgccgg tgacctggtt ctcgatggct gccgggatca
     4141 ggatgtcgac gtcctcggcg atccagtcgc cgcccggctt gacctcgtag cccagcgcct
     4201 tcgccttggc cttgtcgatc ccgccgaagt ggtccgtgat ccccatgagc tgggcaccgt
     4261 ccacgcccga ctccttgcgg aacgtgtagg acgtctggtc ggcctggtcc cacgacgaga
     4321 ccgcgacgac cgtgccgccc atcttcgtgt agagctggat cgcgtactgc gagacgttgc
     4381 cgaacccctg aacggacgcc ttcgtcttcg cgacgtcgat ccccatcttc ctgagcgcct
     4441 cgcgcagcgt gaagaccgcc ccgtagcccg tcgcctcggt gcggccgagc gagccgccca
     4501 tgccgaccgg cttgcccgtg atgaagccgg ggccgcggtg gccgtggatc gtctcccact
     4561 cgtcgagcat ccagagcatg tgctgcggag tcgtcatgac gtccggagcc ggcacgtcga
     4621 tcgcggggcc gacgttcttc gcgacctggc ggacccagcc gcggcagatg ccttcctgct
     4681 cgcgcgcgga gaggttgtgg gggtcgcaga tgacgccgcc cttggcgccg ccgagcggga
     4741 tgtcgacgac cgcgcacttc cacgtcatcc acatggagag agcgcgcacc gtgtcgatcg
     4801 tctcctgcgg gtggaagcgg atgccgccct tgctggggcc gcgggcgtcg ttgtgctgaa
     4861 cgcggaagcc gcggaacacc ttgaccttcc cgtcgtccat gcggaccggg atcgcgaacc
     4921 agtattcgcg gagcggccac cggaggaggt cgcgggtcgg tgcgtcgagc gcgagaatgt
     4981 cggcgctctt gtcgaactgg gtctgggcca tttcgaaggc gttgaacgag cctgtcatct
     5041 ggttctcccc tgtcttcctt cctgtttctg cgtatcctga gggatcggcg gccgtccccg
     5101 aagagacgtc gcagacgtgg gacgcattca aaggaagtgt agggaggcgc ccgcggaccc
     5161 ggccatgagg cgggtgaatc aggccccgaa agccgctccg tttaagatcc gggcgtgatc
     5221 cggttcgaga gggccgtcaa acgctacggc gacaggacgg ccctctcgga cttcaccctg
     5281 gagattcccg ccggcgcgct cttcgcgctc gtgggcccca acggggcggg aaagacgact
     5341 gcgatcggcc ttctcacggg cctcctgtcc ccgacctcgg gccggacctt cctcgcgggc
     5401 atcgacgtcg cccgcgaccc cgtcgccgcc aaggcgaagc tcgggttcgt gccggaccgg
     5461 ccctacctct ggccgaagtg gacgccgcgc gagaccctcc gcttcgtcgg cgccgtcttc
     5521 ggcgcgcgcg gcgccgcgct cgagtcgcgg atcgacgccg agctcgacgc cttcgacctc
     5581 gtggccgtcg cggacgcacg gagcgacacg ctctcccacg gcacgcggca gcgggtcgcg
     5641 ctcgcgcagg cgttcctgca cgaccccgag gtgctcgtcc tcgacgagcc gatggtcggg
     5701 ctcgatccga tcgcccagcg gagcctcggc gaacgcctgc gcacgctcgc cgcccgcggc
     5761 tcggccgtcc tcctgaccac ccaccagctc gctcaggccg aggagctcgc gacggacgcg
     5821 ggcctcctgt tcgacggccg actcctcgcg cgcggagcgc ccggggcgct ccgcaccgct
     5881 tccggtgtca cgggcgcgct ctcggaggtc ttcttcgccc tcgcgggcga aggggacaag
     5941 ccggcgtgaa cgccgtctac cgggccaatg ccgcgcgcgc cgccgcgcga tcccttctcg
     6001 gcgcccgggg ccgcgcccgg attcccgccc tcgccggcct cttttcgctc gccctgttcg
     6061 tggcgctcga ggtcgtcggg ctcgaagcgg cggtccgcgc atcgtcggcc gcgctcgcgt
     6121 tcctccccga gctccggccc ctcttcctcg tcgaacggct cctcgcgggc gcgttcggcg
     6181 ccgccgcggc gctcgtcctc ctcggctcgc tcacgacggc cgtctcgacg ctcttcctct
     6241 ccgaggagct gctcgccctc gccgtcctcc cgattccgca ccgccgcctc ctcgcgcggc
     6301 aggccgccct cacgctcgcg ctcgcgtcgg ccccgtcgct cctactcgcg ctccccgcgc
     6361 ttggggtcgc cgcgtccgcg tcgcacgccc cgctccttgc cgccgcggcc ggcctcctct
     6421 ccgtcgcggg cctgttcctg ctcgccgggt cggtcggaat cgccggcgcg ctcgtcctcg
     6481 tgcgaatcgt ccccgcgcgc cgggcccggc tcttctccgc gcttctctcc gcgctgggcc
     6541 tctcggcggc gctcgtcggc ttccgtgcgg cgcgcccgga gcgtctcctc gatcccgtcg
     6601 aggcgctcgg actcctgcag tcgctcggag cgtccgctcc tcccgcaccc ggagcgagcc
     6661 ccgccggctg ggccgcgcgc agcgcggcgc tcgcactcgc gggcgatccc gcgggcctcc
     6721 ttccgggcgc cctgctcctc gccctc
//
LOCUS       DAOMFY010000219         6725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3722_length_6725_cov_6.302549, whole genome shotgun sequence.
ACCESSION   DAOMFY010000219 DAOMFY010000000
VERSION     DAOMFY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3722_length_6725_cov_6.302549"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLB01_14925"
     CDS             complement(<1..741)
                     /locus_tag="PLB01_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ78647.1"
                     /translation="MTAATRPAPVRFHTTPRFTLESGEVLRDVRQAYVLEGELNEAKD
                     NVVVVVHSLTGSPETLGGWKGSVIGPGKAIDTNKWAVLCPNLLGSCYGTEFKRLLRKE
                     ERRSAHPSVTTRDQAHLIENLLSSLGVSSLALVTGGSLGGMVTLELAASFPSLFRAAV
                     VFAAPAAHTAWAIGWNHVQRRALDAGGPEAGLAVARMAGMLTYRTPEEFEARFGRQGG
                     ADEPFAVQSYLSRHGAKLVARFDAKSYRT"
     gene            complement(738..2054)
                     /locus_tag="PLB01_14930"
     CDS             complement(738..2054)
                     /locus_tag="PLB01_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HQQ78648.1"
                     /translation="MSTTAAEPKPRYRPGTLAVHAAQETADPATNARAVPIYATTSYV
                     FDGPDHAASLFGLREFGNIYTRLMNPTTDVFERRIAELEGGVAAVATASGQAAQTLAL
                     LNLAQAGDTIVSSSALYGGTFTLFQHTLPRLGIRTRFVDANDAAQVRRAIDSTTRAVY
                     VETIGNPKLDVPDFRALADVAHAAGVPLVVDNTFATPLLCRPIEHGADVVVQSATKWI
                     GGHGTAIGGVVVDGGTFDWGSNPRFQGFYRDPEPAYHGLSFADAFGAAAFAVRLRVVL
                     LRDVGASLSPFNSFLFLQGIETLHLRIRRHSENALAVARFLEAHPAVAWVRYPGLPSH
                     PAHATAARYLEGGFGGVLTFGVKGGEAEARAVIKEAKLFSLVANVGDAKSLVIHPWST
                     THEQLAPEDRIVAGVTPDLIRLSVGIEDAEDLTEDLDQALRKAVRT"
     gene            complement(2305..2616)
                     /locus_tag="PLB01_14935"
     CDS             complement(2305..2616)
                     /locus_tag="PLB01_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQQ78649.1"
                     /translation="MKLYVGNLPYSVNDNVLRSTFEAYGAVESARVISDRETGQSKGF
                     GFVEMADGDAQKAMSALNGQELDGRALRVNEARPQEPRTGGGGGRGGYGGGGGYGRER
                     Y"
     gene            2805..3209
                     /locus_tag="PLB01_14940"
     CDS             2805..3209
                     /locus_tag="PLB01_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQQ78650.1"
                     /translation="MTQRAIWDFHGDPRVLERLTPPGKKVRVVERPSAMGAGARVVIR
                     VRQFGVWLTWVSLIEAWEPHSRFVDVQERGPFASWRHEHLFADGRLVDRVTYEVPLAR
                     FGGRLADVSLVRPDIERMFAHRHAVTREALLR"
     gene            3219..5243
                     /locus_tag="PLB01_14945"
     CDS             3219..5243
                     /locus_tag="PLB01_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78651.1"
                     /translation="MIATTLFAMALSTTSISEARTALLAAHGEAQRARIERGVGQAAS
                     LWRASDGDGAAFVAFAKENFASDPKVLDGLLVRFEENLESLDGHMAEIGRDLSRRTVI
                     EMGPILPVDRLFGAWEPSSHVAEDLFAQKIAFVALLNFPLSTLEERIEKGGAWTRRQW
                     AEARLARRFETRVPADVAQGLAQAAADAELYIAEYNVWMHHVVDAKGTRLWPKGLKLL
                     SHWNLRDEIKANYASKDGFGKQRVVAKVMDRIVTQTIPAAVVDNPAVDWDPFTNTVTA
                     APAAEVEAGGRHKSAGADREPDTRYERLLACFRAARAADPYSPTEPTLIRRRFNVDRE
                     IPEARVEALFTEILTSPLVPRIAGVIEKRLGRKLEPFDVWYDGFRPRAAHPEAELDAL
                     TKAKYPTAEAYKADLPRLFEGLGFTKDKARWLADRIVVDPSRGSGHALGAQRKGDVPH
                     LRTRVEPGGMDYKGYNIAVHEMGHNVEQLFSLYEVDHWLLAGVPNNAFTEALAFVFQA
                     RDLQLLGLPAPGAEARRLRALNELWQTWEIAGPALVDMRVWHWMYEHPDATPAALKDA
                     AVAISRDVWNRYYAPVLGGKDATLPGVYSHMVGYFLYLPDYVLGHLIANQVEERFEKS
                     GRPLGEEFERVSKFGSVTPDLWMKNATGEPLSAKPMFRAAEAALEAEAKK"
     gene            5377..5967
                     /locus_tag="PLB01_14950"
     CDS             5377..5967
                     /locus_tag="PLB01_14950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ78652.1"
                     /translation="MSASIHASDRDWPGDERALSRFLSGDVLGFEQIVRHYSGMVFLL
                     AARLVGPAEAEDVVQETFLRAYHGLERFRGEASLKTWLYAIALNRARARHGRLARLRA
                     VFTPGKTREGDVFASLDHAADPALSPEENALLTERRRRLRAAVAALPDEFRAAVVLRD
                     LEGLSYEEVAEVLGVPVGTVRSRLARGRALLKETLS"
     gene            5964..>6725
                     /locus_tag="PLB01_14955"
     CDS             5964..>6725
                     /locus_tag="PLB01_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78653.1"
                     /translation="MSARPDVPRLSDEELDLLISRSLDGDLSPEEEQQLERLVALDPA
                     AARRRADLAALVADVKALPEPATPFALATRVSSNVAERAGRRGSLGSRVGFFPAPGFA
                     KAALVILGIVGVSIAILRPVPKRLAEGPVDVYLYNPATPASPAPRTLIAEAKPARERA
                     NGVAKDLAKEKAFAPEADSRRKADAGETPESAVDGDKKATAKLEGARQNEVAVAADAP
                     VLAEATARPRADRDEAGVLDAVRPQAPAKAMSSGTL"
BASE COUNT         1005 a         2352 c         2414 g          954 t
ORIGIN      
        1 agtcctgtag ctcttcgcgt cgaagcgcgc gacgagcttc gcgccgtggc gcgagaggta
       61 cgactgcacc gcgaacggct cgtccgcgcc gccctggcgg ccgaaccgcg cctcgaattc
      121 ctccggcgtc cggtacgtga gcatgccggc catgcgcgcg accgcgagcc ccgcctccgg
      181 tccgcccgcg tcgagggcgc ggcgctggac gtggttccag ccgatcgccc aggccgtgtg
      241 cgccgcgggc gcggcgaaga cgacggcggc gcggaagagc gagggaaagg acgcggcgag
      301 ctccaacgtg accattccgc cgagggaccc tccggtcacg agagcgaggg aagaaacccc
      361 gagagaagag agaagatttt ctatgaggtg tgcctgatcg cgtgtggtga cggagggatg
      421 cgccgagcgg cgctcttcct ttctaaggag tctcttgaat tccgtgccgt agcacgaccc
      481 cagcagattc gggcacagca cggcccactt gttcgtgtcg atcgccttcc cgggcccgat
      541 cacggagccc ttccacccgc cgagcgtctc gggcgagccg gtcagcgagt ggacgacgac
      601 gacgacgttg tctttcgcct cgttcagctc gccttcgagg acgtaggcct gccgaacgtc
      661 gcgcaggacc tcgccggact ccagcgtgaa gcgcggggtc gtgtggaacc ggaccggcgc
      721 aggacgggtg gcggccgtca cgttcgcacc gccttgcgca gcgcctggtc gaggtcttcc
      781 gtgaggtcct cggcgtcctc gatgccgacc gagagccgga tcaggtccgg cgtgacgccc
      841 gcgacgatcc ggtcctccgg cgcgagctgc tcgtgcgtcg tggaccaggg gtggatcacg
      901 aggctcttcg cgtcgccgac gttcgcgacg agcgagaaga gcttcgcttc cttgatgacc
      961 gcccgggcct cggcctcgcc gcccttcacg ccgaacgtga ggacgccgcc gaacccgcct
     1021 tcgaggtaac gcgccgcggt cgcgtgcgcc gggtgcgacg gcaggccggg atagcggacc
     1081 cacgcgacgg ccgggtgggc ctcgaggaag cgcgcgaccg cgagggcgtt ctcgctgtga
     1141 cgccggatgc gcaggtgcag cgtctcgatc ccctggagga agaggaacga gttgaacggg
     1201 gagagcgagg cgccgacgtc gcgcaggagg acgacgcgca ggcgcacggc gaacgcggcg
     1261 gccccgaacg cgtccgcgaa cgagagcccg tggtacgcgg gctcgggatc gcggtagaag
     1321 ccctggaacc ggggattcga cccccagtcg aacgtcccgc cgtccacgac gacgccgccg
     1381 atcgcggtcc cgtggccgcc gatccacttc gtggcggact ggacgacgac gtcggccccg
     1441 tgctcgatcg gccggcagag gagcggagtc gcgaaggtgt tgtccacgac gagcggaacg
     1501 ccggccgcgt gggcgacgtc cgcgagggcg cggaagtccg gcacgtcgag cttcgggttg
     1561 ccgatcgtct cgacgtagac ggcgcgcgtc gtggagtcga tcgcccgacg aacctgcgcg
     1621 gcgtcgttcg cgtcgacgaa cctcgtcctg atcccgagac gggggagcgt gtgctggaag
     1681 agcgtgaacg tgccgccgta gagcgcggac gacgagacga tcgtgtcacc cgcctgggcg
     1741 aggttgagca gggcgagcgt ctgcgccgcc tgcccgctgg ccgtcgcgac ggccgcgacg
     1801 ccgccctcga gctcggcgat ccggcgctcg aagacgtccg tcgtggggtt catgagacgc
     1861 gtgtagatgt tcccgaactc gcgcaggccg aagaggctcg cggcgtggtc cggcccgtcg
     1921 aacacgtacg acgtcgtcgc gtagatcggg acggcgcggg cgttcgtggc cgggtccgcc
     1981 gtctcctggg ccgcgtggac ggcgagcgta ccggggcggt agcggggttt cggttcggcg
     2041 gcggtcgtgc tcatgggctt ctccttcgtc gttttcgatc tcaagacaca aaaaagaccg
     2101 gccctctcgt gcgagagggc cggtttggga ttccgagtct ggctcgggcc gcttggttcg
     2161 aggacgctta ggtcgtcgag cctccgtcca cctcgcgcga ggcgcaagga atgcacatgc
     2221 acatgcacat gcaggacgca gcggaggcgg agcggacctt catcgtcggg cgacggtaga
     2281 gatggcggag gtcgaagtca agcgtcagta gcgctcgcgg ccgtagccgc cgccgccacc
     2341 gtagccgccg cggccgccac cgccgccggt gcgcggctcc tgcggacggg cctcgttgac
     2401 gcgcagggcg cggccgtcga gctcctggcc gttcagggcg ctcatggcct tctgggcgtc
     2461 tccgtcggcc atctcgacga agccgaagcc cttcgactgg ccggtctcgc ggtcggagat
     2521 gacgcgcgcc gactcgacgg cgccgtaggc ctcgaacgtg gaacggagga cgttgtcgtt
     2581 gacggagtac gggaggtttc caacgtagag cttcatgaga acgagtccta ttcttacttt
     2641 ctattcggag acggtaaagc ggaccgcgtg cgcggccagc ggggcctgga gatggtgggg
     2701 gagatgaacg gcggcgggac gatccttcta caggcgaata tgacagcatc gggaaaagcg
     2761 aaagtcaaat gccgatcctc gaattcgccc tcgaacttcc gggcgtgacg caacgcgcaa
     2821 tctgggattt ccacggcgac ccgcgcgtcc tcgagcgtct cacgcctccc ggcaagaaag
     2881 tccgcgtcgt ggagaggcct tcggcgatgg gcgccggcgc gcgcgtcgtc atccgcgtca
     2941 ggcaattcgg cgtctggctg acgtgggttt ccctcatcga ggcgtgggag ccccattcgc
     3001 gcttcgtgga cgttcaggag agagggccgt tcgcctcctg gcggcacgag cacctcttcg
     3061 cggacggccg tctcgtggac cgcgtgacgt acgaggtccc gctcgcgcgc ttcggcggcc
     3121 gcctggccga cgtctccctc gtgcgccccg acatcgagcg catgttcgcg caccgccacg
     3181 cggtcacgcg ggaggcgctc ctccgatagg atcggcggat gatcgctacg acgctcttcg
     3241 cgatggccct ctccacgacg tcgatctccg aggcccggac cgcgctcctc gcggcgcacg
     3301 gcgaggccca gcgcgcgcgc atcgagcgcg gcgtcggcca ggccgcgtcc ctctggcgcg
     3361 cctcggacgg cgacggcgcc gccttcgtgg cgttcgcgaa ggagaacttc gcgtccgacc
     3421 cgaaggtcct cgacggcctc ctcgtgcggt tcgaggaaaa cctcgaatcg ctggacgggc
     3481 acatggccga gatcggccgc gacctctcgc ggcggacggt catcgagatg gggccgatcc
     3541 tcccggtcga ccggctcttc ggggcgtggg agccctcctc ccacgtcgcc gaggacctct
     3601 tcgcacagaa gatcgcgttc gtcgcgctcc tgaacttccc gctctcgacg ctcgaggagc
     3661 ggatcgagaa gggcggcgcg tggacgcggc ggcagtgggc ggaggcgcgg ctcgcgcgcc
     3721 gattcgagac gcgagtgccg gcggacgtcg cgcaggggct cgcgcaggcc gcggccgacg
     3781 ccgagctcta catcgccgag tacaacgtct ggatgcacca cgtcgtggac gcgaagggga
     3841 cgcggctctg gccgaagggc ctcaagctcc tctcgcactg gaacctccgc gacgagatca
     3901 aggcgaacta cgcgtcgaag gacgggttcg ggaagcagcg cgtcgtcgcg aaggtcatgg
     3961 accgcatcgt gacgcagacg atcccggccg ccgtcgtcga caaccctgcg gtcgactggg
     4021 acccgttcac gaacacggtc acggcggcgc cggctgcgga ggtggaggcc ggcgggcggc
     4081 acaagagcgc gggcgcggac cgcgagccgg acacccgcta cgagcggctc ctcgcctgct
     4141 tccgggcggc acgcgcggcg gacccgtact cgcccacgga gcccacgctc atccggcgcc
     4201 gcttcaacgt ggaccgcgag attcccgagg cgcgcgtcga ggcgctgttc acggagatcc
     4261 tgacgtcgcc gctcgttccc cgcatcgcgg gggtcatcga gaagcgcctc ggcaggaagc
     4321 tcgagccgtt cgacgtctgg tacgacggct tccgcccgcg cgccgcgcac cccgaggcgg
     4381 agctcgacgc gctcacgaag gcgaagtacc cgacggccga ggcctacaag gccgacctgc
     4441 cgcgcctctt cgagggcctc ggattcacga aggacaaggc ccgctggctc gcggaccgga
     4501 tcgtcgtcga cccgtcgcgc ggctccggcc acgcgctcgg cgcacagcgg aagggcgacg
     4561 tcccgcacct ccgcacacgc gtcgagccgg gcggcatgga ctacaagggc tacaacatcg
     4621 ccgtccacga gatgggccac aacgtcgagc agctcttctc gctctacgag gtcgaccact
     4681 ggctcctcgc gggcgtgccg aacaacgcgt tcaccgaggc gctcgcgttc gtcttccagg
     4741 cgcgggacct ccagcttctc ggcctgccgg ctccgggggc cgaggcgagg cggctccggg
     4801 cgctcaacga gctctggcag acttgggaga tcgcggggcc cgcgctcgtc gacatgcgcg
     4861 tctggcactg gatgtacgaa catcccgacg ccactcccgc ggcgctgaag gacgcggcgg
     4921 tcgcgatctc gcgggacgtg tggaaccggt actacgcgcc ggtcctgggc gggaaggacg
     4981 caacgctgcc cggcgtctac tcgcacatgg tcggctactt cctctacctg ccggactacg
     5041 tcctcgggca cctcatcgcg aaccaggtcg aggagcggtt cgagaagtcc gggcggcccc
     5101 tcggcgagga gttcgagcgc gtctcgaagt tcggctcggt cacgccggac ctctggatga
     5161 agaacgcgac gggggagccc ctctccgcga agcccatgtt ccgcgcggcc gaggcggcgc
     5221 tcgaggccga ggcaaagaag tagggaggaa gaaggggcgg gccgggaact tcccggcccg
     5281 cgcggggtcc aacgcgcagg ggacgatgac gtgccggcgc gaatcctcgc cttcgaggct
     5341 tcccgggcaa acgacgcggg tgcgagaatc gctcccgtga gcgcctcgat ccacgccagc
     5401 gaccgggact ggcccggcga cgagcgggcc ctcagccggt tcctgtccgg ggacgtcctc
     5461 gggttcgagc agatcgtgcg ccattactcg gggatggtct tcctgctcgc ggcccgcctc
     5521 gtcggcccgg cggaggccga ggacgtggtc caggagacgt tcctccgcgc gtaccacggg
     5581 ctcgagcgct tccgcggcga ggccagcctc aagacgtggc tctacgcgat cgccctcaac
     5641 cgggcgcgcg cccggcacgg tcggctcgcg cgccttcgcg ccgtgttcac gcccgggaag
     5701 acgcgcgaag gcgacgtttt cgcgtccctc gaccacgccg ccgacccggc gctctcgcca
     5761 gaggagaacg ccctcctgac cgagcgccgc cggcgcctgc gcgcggccgt cgccgccctg
     5821 cccgacgagt tccgcgcggc ggtcgtcctg cgcgacctcg agggcctctc gtacgaggaa
     5881 gtcgccgagg tgctcggcgt tccggtcgga accgtgcgga gccgcctcgc gcgcgggcgc
     5941 gccctgctga aggagacact ttcgtgagcg cccgccccga cgtcccgcgt ctctccgacg
     6001 aggagctcga cctcctcatc tcgcgctccc tcgacggcga cctctcgccg gaggaggagc
     6061 agcagctcga acgtctcgtg gccctcgacc ccgccgccgc ccgccgcagg gccgatctcg
     6121 ccgcgctcgt cgcggacgtg aaggccctgc cggagcccgc gacgccgttt gccctcgcga
     6181 cccgcgtgag ttccaatgtc gccgagcgcg cggggcgccg cggctcgctc ggctcgcgcg
     6241 tcgggttctt cccggcgccc ggtttcgcga aggccgcgct cgtcatcctc ggcatcgtcg
     6301 gcgtttcgat cgcgatcctc cgcccggtgc cgaaacgcct cgcggagggc cccgtggacg
     6361 tctacctcta caacccggcc acgccggcct ccccggctcc acggacgctc atcgccgaag
     6421 cgaagcccgc gcgagagcgg gcgaacggag tcgcgaagga cctcgcgaag gaaaaggcgt
     6481 tcgcgccgga ggccgactcc cgccggaaag cggacgcggg cgaaacgccg gagagcgccg
     6541 tggacggcga caagaaggcg accgcgaagc tcgagggcgc gcgccagaac gaagtcgcgg
     6601 tcgcggccga cgccccggtt ctcgccgaag ccacggcgag gccgcgggcc gaccgggacg
     6661 aggccggcgt gctcgacgcc gttcgcccgc aggctcccgc gaaggcaatg agctccggaa
     6721 cgctc
//
LOCUS       DAOMFY010000220         6720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3726_length_6720_cov_8.725881, whole genome shotgun sequence.
ACCESSION   DAOMFY010000220 DAOMFY010000000
VERSION     DAOMFY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_6720_cov_8.725881"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLB01_14960"
     CDS             complement(<1..817)
                     /locus_tag="PLB01_14960"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQQ78654.1"
                     /translation="MPKPAPKRSTKRTGPKQTLLLVGGAGRMGRRLAPHFRKKGFRVL
                     IADPAGRVPGYVPADLDAASTADVVCVAASLDASPAALEAVLSRAPRGLVFDIASLKT
                     PLLPLYARARKAGVAIASTHPMFGPSAKFRGNDFLVLDCGDAAAAARVSRLFSGEGLR
                     VRRMPVTEHDPWAARTMGLAHVVALACAGALVRLKVDAFDVDGRATTSFRRLLDLVGS
                     LLDQDPALTRAIQTQNPDAPWAAEVLAEEVEAFRAALFAPDPAPLAARIGALRR"
     gene            complement(852..2105)
                     /gene="tyrS"
                     /locus_tag="PLB01_14965"
     CDS             complement(852..2105)
                     /gene="tyrS"
                     /locus_tag="PLB01_14965"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQQ78655.1"
                     /translation="MSTFPPVPEQMDLLLKGVEACEKREELEKKLTRSAKMGKPLRVK
                     VGFDPSAPDLHLGHTVVFRKMRHFQELGHEVIFLIGDFTGMIGDPTGKKATRPQLTKD
                     EILANAETYKAQVFKILDPVKTRVMFNSEWLSALGAEGLVRLCAKYTVAQLLERDDFA
                     KRFREQKPIAVHELLYPLCQGYDSVALDADVELGGTDQLFNLLVGRELMRAWDKEPQC
                     VLTLPLLVGTDGVEKMSKSLNNAIGVTEPPNEIFGKVMSVSDTAMWAYWTLLTDRRPA
                     EIEEMRAAVATGTLHPKKLKMDLGLQIVSDFHGQAAAFAAQEEFERRFAGAGGAVSAD
                     EKTLSSLFPGEDVLERNIGDVLVAVALAPSKNVARQKIKEGAVATSLDGQAWTKVGSP
                     ADLVRFDAPDGIFLRLGKRFVRVTK"
     gene            complement(2137..3603)
                     /locus_tag="PLB01_14970"
     CDS             complement(2137..3603)
                     /locus_tag="PLB01_14970"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ78656.1"
                     /translation="MRSRLAASLLAFGLSTSALAAPPDDAARLAGRAVGDTPLIADTK
                     ELCDTIGGRPVGSAANARAVSWAAAKFKAAGADAVRTEAFPVPFLWLPGTAELSIVAP
                     ENVPLRVVACPAAPSTPGAITAKVVDAGEGTDADYARLGAKAKGAIAVVRSKEMKTAD
                     DLFGEYMRNGPLFDAAKKAGAVAVLLQSTRPRGLLYRHPVGLGRDLAPLPAAMVSREH
                     AARLLRLAEQGDVTVRLNVSNKTGPTYDAQNVVAEIKGMEKPDEVVLLGAHLDSWDLG
                     TGANDNGVNVALLVDALRGIKELGLKPRRTIRFVAFNAEELGMWGSAEYVRAHAKEMG
                     DIAAVVIFDVGSGRTTNFYLNGREDLRKPADAALAGVAGLVGVPHTLEGVDGTDNFDF
                     LLSGVPNLVANQDWAPYLPDYHAESDTFDMVDAREARANVAIASALVWGLANSDARAP
                     RQSRAEVDKLLKETKLDEQMKAMGQWEDWIAGKRGVSK"
     gene            complement(3648..4262)
                     /gene="udk"
                     /locus_tag="PLB01_14975"
     CDS             complement(3648..4262)
                     /gene="udk"
                     /locus_tag="PLB01_14975"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HQQ78657.1"
                     /translation="MTPFVIGIAGGTGSGKTTVAHAIVKTVGEERIAFLSHDAYYRDF
                     VDLPKDILDGKNFDHPDSLETELLVRHLKALKQGMVVETPVYDFKVHRRAAETRRVEP
                     RKVILVDGILIYVEPELRKLFDVKIFVDTDSDVRLIRRIRRDIAERGRTVDSVVKQYE
                     STVRPMHLEFVEPSKRYADLIIPEGGENAVALEFLFARLNAILA"
     gene            complement(4267..5208)
                     /locus_tag="PLB01_14980"
     CDS             complement(4267..5208)
                     /locus_tag="PLB01_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78658.1"
                     /translation="MTTKFSKIAAGAALALLLAAGSAWAGAQGRITGKVTDGKSVPLA
                     DVKITITTKSLSNFKVELKTDKDGKWGTILNDSTIPYHYKFEKQGYIGVEQDKKVPIG
                     ESQVLDVQLLTQQQAIETGVVKAVDDPFVSAYNACVEAYQADNLDLAWTKAQEAVKAG
                     PEKATGYDLAAKVALKKKDWDGVVAMGEKSLSLEADNPPLIGMLMEAYRAKGDKVKFA
                     EYEKKYIAANPDQPDVIYNQAVDLYNKGKFKEAEPLLVKVVEAKPDHAKAHYLLGMCD
                     VNLNKIPEMKKHLSEYLKLEPKGSDASAAKEMLDAFK"
     gene            5355..5957
                     /locus_tag="PLB01_14985"
     CDS             5355..5957
                     /locus_tag="PLB01_14985"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HQQ78659.1"
                     /translation="MRRRLILGLAAALAAAALLFALGFFAGKSASRGPGSAPVVTAVK
                     KIARLATVQMEISDVLRVEEVKTILVFDVPKNATLRLRGRVMGGFDLDKGFDVSADET
                     RRIVKVRLPAAQVLSVDDRLEWFDEKSGFLNPITPDDRTRWTTWARAALAKSAKDAGL
                     FDAAARHAAGLFSDAAAAFGWTAEVTVAGAAVKPAPAPKP"
     gene            complement(5962..6438)
                     /locus_tag="PLB01_14990"
     CDS             complement(5962..6438)
                     /locus_tag="PLB01_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HQQ78660.1"
                     /translation="MYYVIGANGSQYGPVDEATLRAWIAEGRVGAPSLSFKSGEASWA
                     PLSTRDEFRDLLVAVPAATPSAAPAAGAPPVAPPPYPAQQAPLAPGERDWLVALLLSI
                     FLGTFGVDRFYLGQVGLGLLKLFTLGGCGIWWLIDVILIATNTLRDVNGRLPVKRM"
     gene            6528..>6720
                     /locus_tag="PLB01_14995"
     CDS             6528..>6720
                     /locus_tag="PLB01_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78661.1"
                     /translation="MSPEFLRSPEGGDAGRDWGAACGAGRACGADWGAGRDTLCWRAP
                     ESRLRSRTRSSPRWRAEKSR"
BASE COUNT          943 a         2298 c         2370 g         1109 t
ORIGIN      
        1 cgcggcggag ggcgccgatg cgcgcggcga gcggggctgg atcgggcgcg aagagggcgg
       61 cgcggaacgc ctccacctcc tcggcgagga cttcggcggc ccacggcgcg tccgggttct
      121 gcgtctggat cgcccgcgtc agggccgggt cctggtcgag gagcgacccg acgaggtcga
      181 gcaggcggcg gaaggacgtc gtcgcgcggc cgtcgacgtc gaaggcgtcc accttcaggc
      241 gcacgagagc ccccgcgcag gcgagcgcga cgacgtgcgc gaggcccatc gtgcgcgcgg
      301 cccacgggtc gtgctcggtc acgggcatgc gccggacccg gaggccctcg cccgagaaga
      361 gccgcgacac gcgcgccgcc gcggccgcgt cgccgcagtc gaggacgagg aagtcgttgc
      421 cccggaactt cgcggacggc ccgaacatgg ggtgcgtgga cgcgatcgcg acgcccgcct
      481 tgcgggcgcg cgcgtagagg gggaggagcg gcgtcttgag gctcgcgatg tcgaagacga
      541 gcccgcgcgg cgcgcgcgag aggacggcct cgagcgccgc gggcgacgcg tcgagggatg
      601 cggcgacgca gacgacgtcg gccgtcgacg ccgcgtcgag gtccgccggg acgtaacccg
      661 ggacgcgccc cgcgggatcg gcgatcagga cgcggaagcc cttcttccgg aagtgcgggg
      721 cgagacggcg ccccatgcgc ccggccccgc cgacgaggag gagggtctgc ttcggacccg
      781 tgcgcttcgt gctgcgcttc ggcgcgggtt tgggcatcgg aagatttctt agaaggtgaa
      841 gaggggacgg gtcacttcgt gacccgcacg aatcgctttc cgaggcggag gaagatgccg
      901 tcgggagcgt cgaagcggac gaggtcggcc ggcgatccga ctttcgtcca ggcctgccca
      961 tccagcgatg tcgcgaccgc cccttccttg atcttctggc gcgcgacgtt cttgctcggc
     1021 gcgagagcca cggcgacgag cacgtccccg atattccttt ctaagacatc ttctcccgga
     1081 aacaagctgg acagagtctt ctcgtccgcg ctcaccgcgc cccccgcccc cgcgaagcgg
     1141 cgctcgaact cctcttgcgc ggcgaaggcg gcggcctggc cgtggaagtc cgacacgatc
     1201 tggagcccga ggtccatctt cagcttcttc ggatgcagcg tcccggtcgc gacggcggcc
     1261 ctcatctcct cgatctcggc ggggcggcgg tccgtgagga gcgtccagta cgcccacatc
     1321 gccgtatctg agacggacat gaccttgccg aagatctcgt tcgggggctc cgtgacgccg
     1381 atcgcgttgt tcagggactt cgacatcttc tcgacgccgt ccgtcccgac gaggagcggc
     1441 agcgtgagga cgcactgcgg ctccttgtcc cacgcacgca tgagctcgcg cccgacgagg
     1501 aggttgaaga gctggtccgt gccgccgagc tcgacgtccg cgtccagcgc gaccgagtcg
     1561 tagccctggc agagcgggta gaggagctcg tggaccgcga tcggcttctg ctcccggaac
     1621 ctcttcgcga agtcgtcgcg ctcgaggagc tgcgcgaccg tgtacttcgc gcagaggcgg
     1681 acgaggccct cggccccgag cgctgagagc cactcggagt tgaacatcac ccgcgtcttc
     1741 acggggtcga ggatcttgaa gacctgcgcc ttgtacgtct ccgcgttcgc gaggatctcg
     1801 tccttcgtga gctgcgggcg cgtggccttc ttccccgtcg ggtcgccgat catccccgtg
     1861 aagtcgccga tcaggaagat gacttcgtgg ccgagctcct ggaagtggcg catcttgcgg
     1921 aagacgaccg tgtggccgag gtggaggtcg ggcgcggacg ggtcgaagcc gaccttcacg
     1981 cgcaggggct tgcccatctt cgcggaacgg gtcagcttct tctcgagctc ctccctcttc
     2041 tcgcaggcct cgacgccctt gaggaggagg tccatctgtt cggggacggg cggaaaggtg
     2101 ctcacagggg cgcgattgta ggggacggag tcggaatcac ttcgaaacgc cgcgcttccc
     2161 cgcgatccag tcctcccact gccccatcgc cttcatctgc tcgtcgagct tcgtctcctt
     2221 gaggagcttg tcgacctcgg ccctcgactg gcgcggcgcg cgggcgtcgg agttcgcgag
     2281 gccccacacg agcgccgacg cgatcgcgac gttcgcgcgc gcctcgcgcg cgtccaccat
     2341 gtcgaacgtg tccgattccg cgtggtagtc gggcagatag ggcgcccagt cctggttcgc
     2401 gacgaggttc gggacgcccg agaggaggaa gtcgaagttg tccgtcccgt ccacgccctc
     2461 gagcgtgtgc ggcacgccca cgaggcccgc gaccccggcg agcgccgcgt cggcgggctt
     2521 gcgcaggtcc tcgcggccgt tcaggtagaa gttcgtcgtg cggcccgagc cgacgtcgaa
     2581 gatcacgacc gcggcgatgt cgcccatttc ctttgcgtgc gcgcggacgt actcggccga
     2641 gccccacatc ccgagctcct cggcgttgaa ggcgacgaag cggatcgtcc gcctcggctt
     2701 gaggcccagc tccttgattc cgcgcagggc gtccacgagg agcgcgacgt tcacgccgtt
     2761 gtcgttcgcg ccggtcccga ggtcccatga gtcgaggtgc gcgccgagga ggacgacctc
     2821 gtccggcttc tccattccct tgatctccgc gacgacgttc tgcgcatcgt aggtcgggcc
     2881 ggtcttgttc gacacgttca ggcggacggt cacgtcgccc tgctcggcga ggcgcaggag
     2941 tcgcgccgcg tgctcgcgcg agaccatcgc ggccgggagc ggcgcgaggt cgcggccgag
     3001 gccgaccggg tggcggtaga ggaggccgcg cggacgcgtg gactggagca ggaccgccac
     3061 ggctcccgcc ttcttcgcgg cgtcgaacag cgggccgttg cgcatgtact cgccgaacag
     3121 gtcgtcggcc gtcttcatct ccttcgaccg gacgacggcg atcgcgccct tcgccttcgc
     3181 gccgagccgg gcgtagtccg cgtcggtccc ctcgcctgcg tcgacgacct tcgcggtgat
     3241 cgcgccgggc gtggagggcg ccgcggggca cgcgacgacg cgcagcggga cgttctcggg
     3301 tgcaacgatc gagagctccg ccgttcccgg cagccagagg aagggcacgg ggaacgcctc
     3361 cgtgcggacg gcgtcggcgc cggcggcctt gaacttcgcg gcggcccagc tcacggcgcg
     3421 cgcgttcgcg gccgagccca cgggccgacc gccgatcgtg tcgcacagct ccttcgtgtc
     3481 ggcgatcagc ggcgtgtctc ccacggcgcg gccggcgaga cgggcggcgt cgtcgggggg
     3541 cgccgcgagg gcggacgtgg agaggccgaa ggcgaggagg gaggcggcga gacgtgatct
     3601 catgcgccca ttctccaaca cgggcgctcc gggcgcccgg gacgccctca ggcgaggatc
     3661 gcgttcagcc gggcgaagag gaactcgagg gcgacggcgt tctcgccgcc ctccgggatg
     3721 atgaggtccg cgtaccgctt cgagggctcg acgaactcga ggtgcatcgg ccgcaccgtg
     3781 ctctcgtact gcttgacgac gctgtccacc gtgcgtccgc gctcggcgat gtcgcggcgg
     3841 atgcgccgga tgagccggac gtccgagtcg gtgtcgacga agatcttcac gtcgaagagc
     3901 ttccggagct cgggctcgac gtagatgagg atgccgtcca cgaggatgac cttgcgcggc
     3961 tcgacgcgac gggtctcggc cgcccggcgg tgcaccttga agtcgtagac gggcgtctcg
     4021 acgaccatgc cctgcttgag agccttgagg tgcctgacga gaagctcggt ctcgagcgag
     4081 tccgggtggt cgaagttctt cccgtcgagg atgtccttcg ggaggtcgac gaagtcgcgg
     4141 tagtaggcgt cgtgggagag gaacgcgatg cgctcctccc ccaccgtctt cacgatggcg
     4201 tgcgcgacgg tcgtcttgcc cgacccggtg cccccggcga tcccgatgac gaacggcgtc
     4261 acgcggtcac ttgaacgcgt cgagcatttc cttcgcggcg gacgcgtcgg agcccttcgg
     4321 ctcgagcttc aggtactccg agaggtgctt cttcatctcc gggatcttgt tgaggttcac
     4381 gtcgcacatc ccgaggaggt agtgcgcctt cgcgtggtcg ggcttggcct ccacgacctt
     4441 cacgaggaga ggctcggctt ccttgaactt gcccttgttg tagaggtcga ccgcctggtt
     4501 gtagatcacg tcgggctggt cggggttcgc cgcgatgtac ttcttctcgt actcggcgaa
     4561 cttcaccttg tcgcccttgg cgcggtacgc ctccatgagc atgccgatga gcggcgggtt
     4621 gtcggcctcg agggagagcg acttctcgcc catcgcgacg acgccgtccc agtccttctt
     4681 cttgagggcg accttcgcgg cgaggtcgta gccggtcgcc ttctcgggcc cggccttcac
     4741 ggcctcctgc gccttcgtcc aggcgaggtc gaggttgtcg gcctggtagg cctcgacgca
     4801 cgcgttgtag gcggagacga acggatcgtc gacggccttc acgacgcccg tctcgatcgc
     4861 ctgctgctgc gtgaggagct ggacgtccag gacctgggat tcgccgatcg gaaccttttt
     4921 gtcctgctcg acgccgatgt acccctgctt ctcgaacttg tagtggtacg ggatcgtcga
     4981 gtcgttgagg atcgtccccc acttgccgtc cttgtcggtc ttgagctcga ccttgaagtt
     5041 cgagagggac ttcgtcgtga tcgtgatctt gacgtcggcg agcgggacgc tcttgccgtc
     5101 cgtcaccttg cccgtgatgc ggccctgcgc cccggcccag gcgctgcccg cggcgagaag
     5161 cagcgcaagc gcggcgccgg cggcgatctt ggagaatttc gtggtcatgc gtcctccgag
     5221 tccttcccgg actgtccttg catttcgggt gccgattcta accgcctgca cggcggcgtc
     5281 ttcgcccgtc agggcgacgt tttcgggcca ctttcgcacc aaaccgggcc ggacgggact
     5341 agtcttcctg cttcatgcgc cgacgcctga tcctcggcct cgccgccgcc ctcgccgcgg
     5401 ccgccttgct cttcgccctc ggctttttcg cagggaaatc ggcctcgcgc gggccgggct
     5461 ccgcccccgt cgtgaccgcg gtgaagaaga tcgcccgcct cgcgaccgtc cagatggaga
     5521 tctcggacgt cctccgggtc gaagaggtca agaccatcct cgtcttcgac gttccgaaga
     5581 acgcgacact gcgcctgagg ggccgcgtta tgggcgggtt cgacctcgac aagggcttcg
     5641 acgtctcggc cgacgagacg cgccggatcg tgaaggtccg cctcccggcg gcgcaggtgc
     5701 tctccgtcga cgaccggctg gaatggttcg acgagaaatc gggcttcctc aacccgatca
     5761 cgcccgacga ccgaacgcgc tggacgacgt gggcgcgcgc ggccctcgcg aagtccgcga
     5821 aggacgccgg gctcttcgac gccgccgcgc gccacgcggc gggcctcttc tcggacgcgg
     5881 ccgccgcgtt cggctggacg gccgaggtca cggtcgcggg cgcggccgtg aagcccgcgc
     5941 ccgcgccgaa accctgagcc cctacattct cttgacgggc agccttccgt tgacgtcccg
     6001 gagcgtgttc gtcgcgatca ggatcacgtc gatcagccac cagatcccgc agccgcccag
     6061 cgtgaacagc ttcaggaggc cgaggccgac ctggcccagg tagaagcggt cgacgccgaa
     6121 cgtcccgaga aagatcgaga gcagaagcgc cacgagccag tcgcgctcgc cgggcgcgag
     6181 cggcgcctgc tgcgccggat agggcggagg cgcgacggga ggtgcgcccg cggcaggcgc
     6241 ggccgacgga gtagcggccg gcaccgccac gagaaggtcg cggaactcgt cgcgcgtgga
     6301 aagaggcgcc caggacgcct cgcccgactt gaacgaaagg ctcggcgcgc ccacgcggcc
     6361 ctcggcgatc caggcgcgca gcgtcgcctc gtcgaccggt ccgtactggc tgccattggc
     6421 cccgatgacg tagtacatcg cagctcctca cccctagaaa cggattccgg ccccgaaggt
     6481 tctctccggg gccggggcat gctaaccctc tgtctcagtt cctcttcttg tcgcccgagt
     6541 tcctgcgctc tccggaaggc ggagacgccg gccgcgactg gggagccgcc tgcggcgccg
     6601 gacgcgcctg cggcgccgac tggggcgccg ggcgggacac gctctgctgg cgcgcgccgg
     6661 aatcccggct gcgttcgcgc acccgttcgt ctccgcgctg gcgcgccgag aaatcgcggt
//
LOCUS       DAOMFY010000221         6699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3743_length_6699_cov_7.887267, whole genome shotgun sequence.
ACCESSION   DAOMFY010000221 DAOMFY010000000
VERSION     DAOMFY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3743_length_6699_cov_7.887267"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3358)
                     /locus_tag="PLB01_15000"
     CDS             complement(<1..3358)
                     /locus_tag="PLB01_15000"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQQ78662.1"
                     /translation="MLLLSFLPVSRLAAVSNTVVISQVYGGGGNSGATIKNDFVELFN
                     RGTTTVSLNGWSVQYASATGTTWQVTNLTNVSLAPGQYYLVQEAQGAGGTVNLPTPDA
                     TGTIPMSATAGKVALLSSTTALSGACPAGGSIVDFVGFGTTANCFEGSGPTPAPSNTT
                     AVLRAAGGCTETDASSTDFASGAPNPRNTATALAPCSGGSIALSINDVSLAEGNSGTT
                     TFTFTVSLSAPAPAGGVTFNIATQDNTATVADNDYVTRSLIGQSISAGSSTYTFDVTV
                     NGDATVEPTETFFVNVTSVVGTNVTVADGQGAGTITNDDVVLTPIHAIQGSSATSPLV
                     STAVSTSGIVTGLKSNGFFLQAPDVEADADPSTSEGIFVFTSSAPPAAAAVGNSVLVT
                     ATVQEFIPSSDPNSPPVTELSGSPTVLLLSTGNTLPTPVVLSAADTNPAGAIDQLEKY
                     EGMRVSVSSLTVVGPTSGTVNEANATATSNGVFYGVITGIARPFREAGVEVPDPLPSG
                     APCCVPRFDANPERLRVDGDGLVGGTALEVTSGAVVTNLVGPLDYAFRTYTILPDPGS
                     SPGVSGNVSFTPAPIPASDEFTVTSFNMQRFFDTADEPGISDVVLTAAALDKRLNKAS
                     LAIRNVMRTPDIIGVEEMENLATLQAVAARVNADAVAASDPDPGYQAYLVEGNDVGGI
                     DVGFLVKSSRVSVVDVTQVNKDETYVDPNTGNPALLNDRPPLVLRATVTGPSGPAFPI
                     TVIVNHLRSLSGVDDPVDGNRVRTKRRAQAESLANLIQARQSANSAERIISLGDYNAF
                     QVNDGYVDVIGTVKGTPAPATDVVLASADLVNPDLTDLLDLLPAAERYSYSFDGNAQV
                     LDHELVNAALLPFVSRVAYARSNADFPESFRNDANRPERISDHDVAVAYVRFPAPATA
                     VVTGDATICFGASATIQAALTGTPPWSVTWSDGVVQANVPASPATRVVNPTVTTTYTV
                     TAVADANGPGTASGSAQVVVGPPPPPLVMNAPSVVGALSPNRAASVASIAGAAYLWTI
                     TNGTITSGQGTNEITFTAGSAGTPLTLNVGLTLGPCPYGGASATVTVLPAGSAVPFYT
                     VTPCRIVDTRGAAGPAGGPALPAAGGADRTF"
     gene            complement(3502..>6699)
                     /locus_tag="PLB01_15005"
     CDS             complement(3502..>6699)
                     /locus_tag="PLB01_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQQ78663.1"
                     /translation="PPTPFDARAARVKARLADALRRPLAGTVNSQGDRTHRADDVRTT
                     YGYSGLGVRIGVISDSFNALGAASADVASGNLPGPGNPFGRTTPVTVIEEDPTGSDEG
                     RAMLQIVHDLAPDAQLFFATADTSEAGFASNIVALRNAPYNCDIIIDDVFYFDEPVFQ
                     DGIVAQAVNTVTAAGALYFSAAGNEGSVAKGTSGYFEGDFNDTGSPVFTFPGGAKSGT
                     IHNFGGANGDIITTAGPFAYTLNWSDPATASANDYDLFLVSSTGTVKAASTNIQNGAQ
                     APFEIFGPPSFAAGDRLVVFKTAAAAVRAFALNTLRGRLTVTTTGQTHGHSSAVNAFS
                     VAAAPAPAPGPYPAAFSGTSPAENFTADGPRRVFYDPAGVPITPGNVLFGTNGGTVRS
                     KPDITAADGVSTTLPGTSGLNPFYGTSAAAPHAGAIAGLLKSANPALTPAQIRTILTT
                     TAVDIESAGYDNITGFGIVQAFQAMQAVAPTPHSDVALGTTTFAEGTFSNGNGGVDPG
                     ELATLVVQLTNPTATAATAVQATLTTSTPGVTITQGSASYGTLAPSASATNGATPFGF
                     GVSSSVPCSSVITFTLTVSFGGGSSPRVYSVSMTVGKFPAPLAGTLGSAPPTGTGFTS
                     TSGQQTGRLTRNGVGATCAAAKSNPGLTAVTGARQFDAYTFTNTASVSQCVTVTLSAA
                     NGLALYTAAYTSAGFVPANPSTNFLADPGLSGTLQTYSFNVAPATAFTVVVHDINVTP
                     ASGSPYTLTVALATCASGPACTPVTITTPSIASGTINVPYTQSFAATGGSGAVTWSIS
                     GALPTGVTLTGNTLSGTPTQGGSFPISVTATDAAGCTPDTNPYTLSIAGGCPPPPPAL
                     VVTAPAFVGQGSPNRVASIAPIVGATYVWGITNGTITGGQGTPQVTFTAGTAGTPLTL
                     TVNATIGVCPAGGGFANVTVLPIGSSVQFYTVTPCRIVDTRKPIGPVGGPALASGGPD
                     RAFALAGACGIPAGASSVSANVTVTDPAAPGNLVIYRGDGVLPGTSTINFGVGQTRAN
                     NAQLQLALDGSGTVKVSNASAGPVHFILDVNGYFQ"
BASE COUNT          956 a         2223 c         2512 g         1008 t
ORIGIN      
        1 cgaacgtgcg gtccgctccg cccgcggcgg gcagcgccgg accgcccgcc ggtccggccg
       61 cgccgcgcgt gtcgacgatc cggcagggcg tcaccgtgta gaacggaacc gcggagccgg
      121 ccggcaggac cgtcacggtc gcggacgcac cgccgtacgg acacggcccg agcgtgaggc
      181 ccacgttcag ggtcagcggc gttccggccg atcccgcggt gaacgtgatc tcgttcgtgc
      241 cctgtccgga cgtgatcgtc ccgttcgtga tcgtccagag gtacgcagcc cccgcgatcg
      301 acgcgacgct cgcggcgcgg ttcggcgaga gagctcccac gacggagggt gcgttcatga
      361 cgagcggcgg cggcggcggg ccgacgacga cctgcgcgct ccccgaggcg gtgcccggcc
      421 cgttggcgtc cgcgacggcc gtcacggtgt acgtcgtcgt cacggtcggg ttcacgacgc
      481 gcgtcgccgg gctcgccgga acgtttgcct gcacgacgcc gtcggaccag gtcacactcc
      541 agggcggagt ccccgtgaga gcggcctgga tcgtcgctga ggcgccgaag cagatcgtgg
      601 cgtcgccggt caccacggcc gtcgccggcg cggggaatcg cacgtacgcg acggccacgt
      661 cgtggtcgga gatccgctcg ggccggttcg cgtcgttccg gaagctctcg gggaagtccg
      721 cgttgctccg ggcgtacgcg acgcgggaga cgaagggaag cagcgcggcg ttcacgagct
      781 cgtggtcgag gacctgggcg ttgccgtcga aactgtacga gtagcgctcg gccgccggga
      841 ggaggtcgag gaggtcggtc aggtccgggt tcacgaggtc ggcgctcgcg aggacgacgt
      901 ccgtggcggg cgcgggcgtt cccttgaccg tgccgatcac gtccacgtac ccgtcgttca
      961 cctggaacgc gttgtagtcg ccgagagaga tgatccgctc cgcggaattc gcgctctgcc
     1021 gcgcctggat gaggttcgcg agggactcgg cctgcgcgcg gcgcttcgtg cggacgcggt
     1081 tgccgtcgac cgggtcgtcg acgcccgaga gcgagcgcag gtggttcacg atgaccgtga
     1141 tcggaaacgc cgggcccgag ggacccgtga cggtcgcgcg gaggacgagc ggcggccggt
     1201 cgttcaggag ggccgggttc ccggtgttcg gatcgacgta cgtctcgtcc ttgttgacct
     1261 gcgtgacgtc cacgacgctc acgcgggagg acttcacgag gaagccgacg tcgatcccgc
     1321 cgacgtcgtt cccctcgacg agataggcct ggtagccggg atccgggtcg ctcgcggcaa
     1381 cggcgtccgc gttgaccctc gccgcgaccg cctggagcgt cgcgaggttc tccatctcct
     1441 cgacgccgat gatgtcgggc gtccgcatca cgttgcggat cgcgagcgag gccttgttca
     1501 ggcgtttgtc cagcgccgcc gccgtgagga cgacgtcgct aatgccaggc tcgtcggcgg
     1561 tgtcgaagaa ccgctgcatg ttgaacgagg tcaccgtgaa ctcgtcgctc gcgggaatcg
     1621 gagcgggcgt gaacgacaca ttgccgctga cgccgggcga ggagcccggg tccggcagga
     1681 tcgtgtacgt gcggaaggcg tagtcgaggg gcccgacgag gttcgtgacg acggcgccgg
     1741 acgtgacctc gagcgccgtg ccgcccacca ggccgtctcc gtccacccgc agcctctccg
     1801 ggttcgcgtc gaagcgcggg acgcagcagg gcgcgccgga gggcagcggg tccgggacct
     1861 cgacgcccgc ctcgcggaac ggccgcgcga tgccggtgat gaccccgtag aagacgccgt
     1921 tcgacgtcgc cgtcgcgttc gcctcgttga ccgtgccgga cgtcgggccg acgaccgtca
     1981 gcgacgacac cgacacgcgc atgccctcgt atttctcgag ctggtcgatc gccccggccg
     2041 gattcgtatc ggccgccgaa aggacgacgg gagttggaag cgtattcccc gtcgacagga
     2101 gcaggaccgt cggcgagccc gacagctccg tcacgggcgg gctgttcggg tccgacgacg
     2161 gaatgaactc ctggacggtc gcggtcacga ggaccgaatt gcccaccgcg gccgcggccg
     2221 gaggcgcgga cgacgtgaac acgaagatcc cctccgacgt gctcgggtcc gcgtccgctt
     2281 cgacgtcggg cgcctggagg aagaagccgt tcgacttcaa cccggtcacg atgcccgacg
     2341 tcgaaacggc ggtgctcacg aggggcgagg tcgcactgct cccctggatc gcgtggatcg
     2401 gcgtcagcac gacgtcgtca ttcgtgatcg tcccggcgcc ctggccgtcc gcgacggtca
     2461 cgttcgtgcc gacgacgctc gtgacgttca cgaagaacgt ctcggtgggc tcgacggtgg
     2521 cgtcgccgtt caccgtgacg tcgaacgtgt aggtcgagct gcccgcggag atgctctggc
     2581 cgatcaacga acgcgtcacg tagtcgttgt cggcgaccgt cgcggtgttg tcctgcgtcg
     2641 cgatgttgaa cgtcacgccg cccgcaggcg cgggcgcgga caggctgacc gtgaacgtga
     2701 acgtcgtcgt cccggagttc ccttccgcga gcgagacgtc gttgatcgag agcgcgatcg
     2761 agccgcccga gcacggggcg agcgccgtcg ccgtgttgcg cgggttcggt gcgcccgacg
     2821 cgaagtccgt gctgctggcg tcggtctccg tgcagccgcc cgcggccctc aggaccgccg
     2881 tcgtgttgga cggagcggga gtcggccccg agccctcgaa acagttcgcg gtcgtcccga
     2941 agccgacgaa gtcgacgatc gacccgccgg ccgggcaggc gcccgagagg gcggtggtgg
     3001 agctcaggag ggcgaccttg cccgccgtgg cgctcatcgg gatggtgccg gtggcgtcgg
     3061 gcgtaggcag gttcaccgtt ccgccggcgc cctgcgcctc ctggacgagg tagtactgcc
     3121 ccggcgcgag ggagacgttc gtcaggttcg tgacctgcca cgtcgttccc gtcgcggacg
     3181 cgtactggac ggaccagccg ttcaggctga ccgtcgtcgt cccgcggttg aagagctcga
     3241 cgaagtcgtt cttgatcgtc gcgccggagt tcccgccgcc gccgtacacc tggctgatga
     3301 cgacggtgtt cgaaacggcg gcgagccgcg agaccggcag gaaggacaga aggagcacaa
     3361 ggacacgaag gatcctggag cgcacaaaca cgcgtctact cctcggcggg cattgcgccg
     3421 cgcccggatc ccgagcctac cccagaaagt gaaaggccgc ggcgaggacc gcggcccgag
     3481 tctgccgaga acgcgtcgcg gctattggaa gtagccgttc acgtcgagga tgaagtggac
     3541 cggcccggcc gacgcgttgc tcaccttcac ggtccccgac ccgtcgagcg caagctggag
     3601 ctgcgcgttg ttcgcgcgcg tctgcccgac accgaagttg atcgtgctcg ttcccgggag
     3661 aacgccgtcg ccccggtaga tcacgagatt tcccggcgcg gccggatccg tcaccgtgac
     3721 gttcgcggac accgaggatg cgcccgccgg aatgccgcac gcgcccgcga gcgcgaatgc
     3781 ccggtccggg ccgccggacg cgagagcggg accgccgacc ggaccgatcg gtttacgggt
     3841 gtcgacgatc cggcagggcg tgaccgtgta gaactgcacc gacgacccga tcggcagcac
     3901 ggtgacgttc gcgaacccgc cgccggccgg gcagacgccg atcgtggcgt tcacggtgag
     3961 cgtcagcggc gtgcccgccg tgccggcggt gaaggtcacc tgaggagtcc cctggccgcc
     4021 cgtaatcgtc ccgttcgtga ttccccagac gtacgtcgcc ccgacgatcg gcgcgatgct
     4081 cgcgacgcgg ttcggggacc cctggccgac gaacgccggc gcggtcacga cgagcgcggg
     4141 cggcggcggc gggcagccgc ccgcgatcga gagcgtgtag ggattcgtgt ccggcgtgca
     4201 gcccgcggcg tccgtcgccg tcaccgagat cgggaagctc ccgccctgcg tcggcgtccc
     4261 cgagagggtg ttgcccgtca gcgtcacgcc ggtggggagc gcgccggaga tcgaccacgt
     4321 gacggcgccg ctcccgccag tggccgcgaa cgactgggtg taggggacgt tgatcgttcc
     4381 gctcgcgatc gacggcgtcg tgatggtgac cggcgtgcag gcgggcccgg aggcgcacgt
     4441 cgcgagcgcg accgtgagcg tgtacggcga accggaagcc ggcgtgacgt tgatgtcgtg
     4501 cacgacgacg gtgaacgccg tcgccggggc cacgttgaac gagtacgtct gcagggtccc
     4561 cgacagcccc gggtccgcga ggaagttcgt gctcgggttg gcgggcacga agccggcgct
     4621 cgtatacgcc gccgtgtaga gagcgagccc gttcgccgcc gagagcgtca cggtgacgca
     4681 ctgggagacc gaggccgtgt tcgtgaacgt gtacgcgtcg aactgccgcg cgcccgtcac
     4741 tgcggtcagg cccgggttgc tttttgccgc ggcgcaggtc gcaccgacgc cgttgcgcgt
     4801 gaggcgcccc gtctgctggc cgctcgtgga cgtgaacccg gtcccggtgg gaggcgccga
     4861 tccgagcgtc cccgcgagcg gcgcggggaa ttttccgacc gtcatggaga cggagtacac
     4921 ccgcgggctc gatccgccgc cgaaggagac cgtgagcgtg aacgtgatca cactgctgca
     4981 gggcacgctc gacgagacgc cgaagccgaa cggagtggcg ccgttcgtcg cgctcgcgga
     5041 tggtgcgagc gtcccgtagg aggccgagcc ctgggtgatc gtgacgcccg gcgtcgacgt
     5101 cgtgagcgtc gcctggaccg ccgtcgcggc cgtcgccgtg gggttcgtga gctggacgac
     5161 gagcgtcgcg agctcgccgg gatcgacgcc gccgttcccg ttcgagaacg tcccttccgc
     5221 gaacgtggtc gtgccgaggg ccacgtcgga atggggcgtg ggagccacgg cctgcatggc
     5281 ctggaacgcc tggacgatgc cgaaacccgt gatgttgtcg tagcccgccg actcgatgtc
     5341 gaccgcggtc gtcgtgagga tcgtgcggat ctgggcgggc gtgagcgcgg ggttcgccga
     5401 cttgaggagg cccgcgatcg cgcccgcgtg cggcgcggcc gccgaggtcc cgtagaacgg
     5461 gttcagtccg gacgtgcccg gcagcgtcgt cgaaacgccg tccgccgccg tgatgtcggg
     5521 cttcgagcgg accgtcccgc cgttcgtccc gaacaggacg tttccgggcg tgatcgggac
     5581 gcccgccggg tcgtagaaca cgcggcgcgg tccgtccgcg gtaaagttct cggccgggct
     5641 ggtcccgctg aacgccgccg gataggggcc gggcgccggg gcgggcgccg ccgccacgct
     5701 gaacgcgttc accgccgagg agtggccgtg cgtctggccc gtcgtcgtga cggtcagtcg
     5761 gccgcggagc gtgttgaggg cgaacgcgcg tacggcggcg gccgccgtct tgaagacgac
     5821 gaggcgatcg cccgcggcga acgacggcgg tccgaagatc tcgaacgggg cctgcgcccc
     5881 gttctggatg ttcgtggagg ccgccttcac cgtgcccgtg gagctgacga ggaagaggtc
     5941 gtagtcgttc gcggaagcgg tcgcgggatc cgaccagttc agcgtgtagg cgaacggccc
     6001 ggccgtcgtg atgatgtcgc cgttggcgcc gccgaagttg tggatcgtcc ccgacttggc
     6061 gcctcccggg aacgtgaaga cgggagagcc cgtatcgttg aagtcgccct cgaagtagcc
     6121 ggacgtgccc ttggcgacgc tgccctcgtt gcctgcggcc gagaagtaga gcgctccggc
     6181 ggccgtcacg gtgttgacgg cctgggcgac gatgccgtcc tggaagaccg gctcgtcgaa
     6241 gtagaagacg tcgtcgatga tgatgtcgca gttgtacggc gcgttgcgca gagccacgat
     6301 gttcgacgcg aaacccgcct cgctcgtgtc cgcggtcgcg aagaagagct gggcgtcggg
     6361 cgcgaggtcg tggacgatct ggagcatcgc ccgcccttcg tcgctgcccg tcgggtcttc
     6421 ctcgatgacg gtgacggggg tcgtgcgtcc gaagggattg cccggcccgg gcaggtttcc
     6481 gctcgcgacg tcggcggagg cggcgccgag cgcgttgaag ctgtcggaga tcacgccgat
     6541 cctcacgccg aggccggagt agccgtaggt cgtacggacg tcgtcggcgc ggtgcgtgcg
     6601 gtcgccctgc gagttcaccg tcccggcgag cggccggcga agggcgtcgg cgagacgcgc
     6661 cttcacgcgc gcggcacgcg cgtcgaacgg cgtgggggg
//
LOCUS       DAOMFY010000222         6678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3756_length_6678_cov_7.338366, whole genome shotgun sequence.
ACCESSION   DAOMFY010000222 DAOMFY010000000
VERSION     DAOMFY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3756_length_6678_cov_7.338366"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLB01_15010"
     CDS             complement(<1..906)
                     /locus_tag="PLB01_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78664.1"
                     /translation="MPHDALGCIRRAGSDPGASRGRPLVRAAPGGGTRLTPPRRATLA
                     FGATLFLTALSLWAIPDTAAGATRVAGFLLAAAGLVLGWMRPSLATCLVAAAAPLGMG
                     LPRLLGDHGGAPLLPVLAFAALGSALARRLLRDERSLLPPKLVVFASAFLVLAGGSAV
                     ASSVRGETLFLLLRGRVDPLVVNVLGMTAAERTRDAALVFLGVVLLLLALDAFARLSL
                     DPDGRDRLLLWVAAGGGGALLVAAVDRLRPLDPLFQPWSAMQRRAATFTDPNALGVGI
                     GLLVPLLFAALAGRGAASDGARRAAA"
     gene            complement(801..4007)
                     /gene="carB"
                     /locus_tag="PLB01_15015"
     CDS             complement(801..4007)
                     /gene="carB"
                     /locus_tag="PLB01_15015"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQQ78665.1"
                     /translation="MPKRTDLKSILVLGSGPIVIGQACEFDYSGTQACRALRKEGYRV
                     VLVNSNPATIMTDPEFADATYIEPLERDTVAAIIAQEKPDALLPTVGGQTALNLAVAL
                     DEAGALAKHGVETLGASIHSVKLGEDRLLFKQAMQEVGVDVLKSGMARSLEEARAIAQ
                     PFGYPVIIRPSFTLGGSGGGIAFNKDDFDGIVKRGLTLSPVHEVLVEESVLGWKEFEL
                     EVMRDAADTFVVVCSIENLDPMGVHTGDSITIAPQMTLSDVEYQAMRDDAKKVIRAVG
                     VATGGSNIQFAVHPKTGRRIVIEMNPRVSRSSALASKATGFPIAKIAALLAVGYRLDE
                     IPNDITKKTPASFEPTLDYVVTKIPRFTFEKFPGADSTLGVQMKSVGEVMALGRTFAE
                     SFGKALRSLETGRTGWVDAVPAPAPKPDALRVPRPERIYAVLAALRHGTSVEAVAEVT
                     GIDRWFLDRFAEALAVEARVKAAGPAALEDADLLREAKRSGYSDPDLAALTGRPAAAV
                     ATARERLSVTPVFSRVDTCAAEFESTTPYLYSCYETEDEAGVTSKEKVIVLGSGPNRI
                     GQGLEFDYCCVHAAEAITEAGYESVMVNCNPETVSTDYDTSDRLYFEPLTFEDVMAVV
                     KREKPKGVLVQFGGQTPLKLAHALENAGVPILGTQPEAIDRAEDRERFGELLKEVNLQ
                     APPWGVAHTAEEGMRVGRKLGFPLLVRPSYVLGGRAMRIAYDEDALKHVLVEALAAFP
                     DRPILIDRFLEDAYELDVDALGDGEHVVVAGVMQHIEQAGIHSGDSYAVLPPYTRVPR
                     AALGEIRQATVKLGLALKVVGLMNVQYAIRGSELYVLEVNPRASRTVPFVAKTTGVPF
                     ARLAARLCLGETLPEVGLTEEPPVLGFSIKAPVFPFRKFEGVDTILGPEMRSTGEVMG
                     RDRGFGLAFLKAALGAGIRLPEKGAAFLSVHDGDKDALLPLARELHALGFTLLATGGT
                     ARALQKGGLAPRTVLKVNEGRPNIVDLMINGSVDLVVNTPLGKESFYDEVAIRRTALE
                     RDIPCLTTLSAASAALEAIRARAAGALSYAPLQEEVRV"
     gene            4117..4893
                     /locus_tag="PLB01_15020"
     CDS             4117..4893
                     /locus_tag="PLB01_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQQ78666.1"
                     /translation="MKKSLWNTFVSFGLAGAMVASAPAALSASKKTPTPAAEAPAAKT
                     SSDKSAKTEKVNLNTATEKEIAAAKGVGDKLAKELVAGRPYKTWDEVSKVKGVGTGKK
                     LDSLKKVLKLSGGEAAAAKEEKPAKEEKKDTKKKSSSKGDEAAAPSAAGAAAGGAAAG
                     SSAAAGKASASSEPSSPSKKSSSSSSSSSSLAPGTKININTASAEELDKLPEIGPVKA
                     QAIVDYRKANGKFATPEDIMKVSGIKEGTYAKIRDYIVVR"
     gene            4906..5313
                     /locus_tag="PLB01_15025"
     CDS             4906..5313
                     /locus_tag="PLB01_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HQQ78667.1"
                     /translation="MSLLDDILREVGGASGASADPSQHSLATDLLGMLSGGGMSRGLG
                     GLVDMFNQKGLGDVMSSWVSTGQNLPISADQIQSVLGSAQVQALAAKAGIDPQMASAA
                     IARILPLLVDKATPNGQLPAGGILDAISGFFKA"
     gene            5332..5772
                     /locus_tag="PLB01_15030"
     CDS             5332..5772
                     /locus_tag="PLB01_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HQQ78668.1"
                     /translation="MTEINFHPAAPILRVVNLEASLAYYSSALGFAKDWGDRRFASVS
                     RGRCTLFLAEGDQGHPGGWVYVGVGDAAALHDELRAKGAKVRQPPTNHPWALEIQVED
                     LDGNVLRFGSDSLEGQPFGEWLDMRGARWIRSAAGAFTRREEES"
     gene            5769..6164
                     /locus_tag="PLB01_15035"
     CDS             5769..6164
                     /locus_tag="PLB01_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78669.1"
                     /translation="MSPIRTTLGVCAGAFLVLSSAAHSLLGWPEMAAALSKTNAPAEL
                     VRGLAIGWHFGGVAMLVFGVLVLQLFLRARRDDSVSLGPALLIGGAYAAFGLGALAIS
                     GLNPFYFVFIAPGAALVLASLRKSSNRRR"
     gene            complement(6136..>6678)
                     /locus_tag="PLB01_15040"
     CDS             complement(6136..>6678)
                     /locus_tag="PLB01_15040"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQQ78670.1"
                     /translation="WGCASAPPPPPPAPEPAPAPEPAPAPQAAEVSWIVPKTVNVRET
                     PSTKAATVAKAAKGIRLTSFEEKDGWVRVKLPDTRIGWVKKDLLKRDDGCLPDRKEVL
                     IEPPMLRVSDAPGAKGKVVVEADIDEAGSVKAVRVVTNETGSPERAEAVKAEVKTVKF
                     QPPVRRCKVTGFTYVYSRTF"
BASE COUNT         1050 a         2226 c         2424 g          978 t
ORIGIN      
        1 cgcggccgcg cggcgggctc cgtccgaggc ggcgccgcgc ccggcgagtg cggcgaagag
       61 cagcgggacg aggaggccga tcccgacgcc gagcgcgttc gggtcggtga acgtcgcggc
      121 ccggcgctgc atcgccgacc acggctggaa cagggggtcg agcggccgaa ggcggtccac
      181 ggcggccacg aggagggcgc ctccgccccc ggcggcgacc cagagcagca atcggtcgcg
      241 gccgtcgggg tcgagcgaga ggcgtgcgaa cgcgtccagc gcgaggagga gcaagacgac
      301 cccgagaaag acgagggccg cgtcgcgcgt gcgttcggcc gcggtcatcc cgaggacgtt
      361 cacgacgagc gggtcgacgc gcccgcggag gaggaggaac agcgtctcgc cgcgaaccga
      421 ggacgcgacc gcggagccgc cggcgaggac gagaaacgcg gacgcgaaga cgacgagctt
      481 cgggggaagg agcgagcgct cgtcgcgaag gagacgccgg gcgagtgcgc tccccagggc
      541 cgcgaaggcg agcacgggca ggagcggggc tccgccgtgg tcgccgagaa ggcgggggag
      601 gcccattccg aggggggcgg ccgccgccac gaggcacgtc gcgagggagg gccgcatcca
      661 gccgaggacg aggcccgcgg cggcgagaag gaatccggcg acccgggtcg cgccggcggc
      721 ggtgtccggg atcgcccaga gggagagcgc ggtcaggaag agcgtcgcgc cgaaggcgag
      781 ggtcgcccgg cgcggcgggg tcagacgcgt acctcctcct ggagcggcgc gtacgagagg
      841 gcgcccgcgg ctcgcgcccg gatcgcttcc agcgcggcgg atgcagccga gagcgtcgtg
      901 aggcacggga tgtcgcgctc gagcgccgtg cggcggatcg caacctcgtc gtagaaggac
      961 tccttgccga gcggcgtgtt cacgacgagg tccacggacc cgttgatcat gaggtcgacg
     1021 atgttcgggc ggccctcgtt caccttcagg acggtgcgcg gcgcgaggcc gcccttctga
     1081 agcgccctcg cggtgccccc cgtcgcgagg agcgtgaagc cgagtgcgtg cagctcgcgg
     1141 gcgagaggca ggagcgcgtc cttgtcgccg tcgtggacgg acaggaacgc ggcgcccttc
     1201 tcggggagcc ggatgcccgc gccgagcgcg gccttcagga acgcgaggcc gaagccgcga
     1261 tcgcggccca tgacctcgcc cgtcgaacgc atctcgggac cgaggatcgt gtcgacgccc
     1321 tcgaacttcc ggaacgggaa gacgggcgcc ttgatggaga acccgaggac gggcggctcc
     1381 tcggtgaggc cgacctcggg aagcgtttcg ccgaggcaga ggcgcgccgc gaggcgcgcg
     1441 aacggcacgc ccgtcgtctt cgcgacgaag ggcacggtgc gcgacgccct cgggttcacc
     1501 tcgagcacgt agagctcgct gccgcggatc gcgtactgga cgttcatgag gccgacgacc
     1561 ttcagggcga ggccgagctt cacggtggcc tgacggatct cgccgagcgc ggcacgcggg
     1621 acgcgcgtgt agggcgggag gacggcgtac gaatccccgg agtggatgcc cgcctgctcg
     1681 atgtgctgca tgacgccggc cacgacgacg tgctcgccgt cgccgagggc gtcgacgtcc
     1741 agctcgtagg cgtcctcgag gaagcggtcg atgaggatcg ggcggtccgg gaacgcggcg
     1801 agcgcctcga cgagaacgtg cttgagcgcg tcctcgtcgt acgcgatgcg catcgcgcgc
     1861 ccgccgagca cgtagctcgg gcggacgagg agcgggaagc cgagcttgcg gcccacgcgc
     1921 atcccttcct cggcggtgtg cgcgacgccc cagggcggcg cctggaggtt gacttccttg
     1981 aggagctcgc cgaagcgctc gcggtcctcc gcgcggtcga tcgcctcggg ctgcgttccg
     2041 aggatcggca cgccggcgtt ctcgagcgcg tgtgcgagct tgagcggcgt ctggccgccg
     2101 aactgaacga ggacgccctt cggcttctcg cgcttcacga cggccatgac gtcctcgaac
     2161 gtgagcggct cgaagtagag cctgtcggac gtgtcgtagt ccgtcgaaac cgtctcgggg
     2221 ttgcagttca ccatgacgga ctcgtagccc gcctccgtga tcgcctcggc cgcgtggacg
     2281 cagcagtagt cgaactcgag gccctgcccg atccggttcg gcccggagcc gagcacgatg
     2341 accttttcct tcgacgtgac gccggcctcg tcctccgtct cgtagcagga gtagaggtag
     2401 ggcgtcgtcg actcgaactc ggccgcgcag gtgtcgacgc ggctgaagac gggcgttacg
     2461 gagagccgct ctcgcgccgt cgcgacggcg gcggcgggcc ggccggtgag cgcggcgagg
     2521 tcgggatccg agtagccgct ccgcttggcc tcgcgcagga ggtccgcgtc ctcgagcgcc
     2581 gcgggcccgg ccgccttgac gcgcgcctcg acggcgagcg cctccgcgaa gcggtcgagg
     2641 aaccaccggt cgattcccgt gacttccgcg accgcctcga cgctcgtccc gtgccgcagc
     2701 gccgcgagga ccgcgtagat gcgctccggg cgcgggacgc ggagcgcatc gggcttgggc
     2761 gcgggcgccg ggacggcgtc cacccagccc gtccggcccg tctcgagcga gcgcagggcc
     2821 ttcccgaacg actcggcgaa cgtccggcca agcgccatga cctcgcccac ggacttcatc
     2881 tggacgccga gcgtcgagtc ggcgccgggg aacttctcga acgtgaagcg cgggatcttc
     2941 gtgacgacgt agtcgagcgt cggctcgaac gaggccggcg tcttcttcgt gatgtcgttc
     3001 ggaatctcgt cgagccggta gccgacggcc aggagcgccg cgatcttcgc gatcgggaag
     3061 cccgtggcct tcgaggcgag ggcggacgac cgcgagacgc gcgggttcat ctcgatgacg
     3121 atccggcggc ccgtcttcgg gtgcaccgcg aactggatgt tcgagccgcc cgtcgcgacg
     3181 ccgacggcgc ggatgacctt cttcgcgtcg tcgcgcatcg cctggtactc gacgtccgag
     3241 agcgtcatct gcggggcgat cgtgatcgag tcgcccgtgt ggacgcccat cgggtccagg
     3301 ttctcgatcg agcacacgac gacgaacgtg tcggccgcgt cgcgcatgac ctccagctcg
     3361 aactccttcc acccgagaac ggactcctcg acgaggacct cgtggacggg ggagagcgtg
     3421 aggccgcgct tcacgatgcc gtcgaagtcg tccttgttga aggcgatgcc gccgccggaa
     3481 ccgccgagcg tgaacgacgg ccggatgatg acggggtaac cgaagggctg cgcgatggcc
     3541 cgggcctctt ccaggctccg ggccatgccg gacttcaaga cgtcgacgcc gacctcctgc
     3601 atcgcctgct tgaagaggag gcggtcctcg ccgagcttca cggagtggat ggacgccccg
     3661 agcgtctcga cgccgtgctt cgcgagggcg ccggcctcgt cgagcgccac ggcgaggttc
     3721 agggccgtct gtccgccgac ggtcgggagg agcgcgtccg gcttttcctg cgcgatgatc
     3781 gcggcgaccg tgtcgcgctc cagcggctcg atgtacgtcg cgtccgcgaa ctcagggtcc
     3841 gtcatgatcg tggcggggtt cgagttcacg aggacgaccc ggtagccctc cttccgaagg
     3901 gcccggcagg cctgggtccc ggagtagtcg aactcgcagg cctggccgat cacgatcggg
     3961 cccgaaccca gcacgaggat tgacttgaga tcggtccgct tcggcatcgg tcgcggagtt
     4021 tacgggatgc ggcggcgccc cgctggcgcc cgcggaccgc gcgagataga gtctcgcgag
     4081 agcgagaatc gaagagaaag aaggagattt ctgcgaatga aaaagagtct ttggaacacg
     4141 ttcgtctctt ttgggctcgc cggggcgatg gttgcgtcgg ctcccgccgc tctttccgct
     4201 tccaagaaaa ctcccactcc cgccgccgag gcgccggccg caaagacgtc ctccgacaag
     4261 tccgccaaga ccgagaaggt caacctgaac acggccaccg agaaggaaat tgcggcggcc
     4321 aagggcgtcg gggacaagct cgccaaggag ctcgtcgcgg gccgtcccta caagacgtgg
     4381 gacgaggtct cgaaggtcaa gggcgttggc acgggcaaga agctcgactc gctcaagaag
     4441 gtcctcaagc tcagcggcgg cgaggccgcc gcggcgaagg aagagaagcc cgcgaaggaa
     4501 gagaagaagg acacgaagaa gaagagttct tcgaagggtg atgaggcggc ggccccctcg
     4561 gcggccggcg cagcggccgg tggcgccgcg gcgggaagct ctgccgcagc gggcaaggct
     4621 tcggcttcct ccgagccctc ttcaccttct aagaaatctt cttcttcctc ttcctcttct
     4681 tcttcgctcg cgcccggtac gaagatcaac atcaatacgg cctcggccga agagctcgac
     4741 aagctgcccg agatcggacc cgtgaaagcg caggcgatcg tcgactaccg caaggccaac
     4801 gggaagttcg cgacgcccga ggacatcatg aaggtgtccg gcatcaagga aggcacgtac
     4861 gcgaagatca gggactacat cgtggtccgg tagagagagc gcgaaatgag tcttctcgac
     4921 gacatcctca gggaagtcgg cggcgcctcc ggcgcctccg cggacccgtc ccagcacagc
     4981 ctcgcgaccg acctcctggg catgctctcg ggcggcggga tgtcgagggg cctcggcggc
     5041 ctcgtcgaca tgttcaacca aaaagggctc ggtgacgtca tgagctcgtg ggtgtcgacg
     5101 gggcagaacc tcccgatctc ggcagaccag atccagtccg tactcgggag cgcacaggtg
     5161 caggctctcg cggccaaggc aggcatcgac ccacagatgg cgagcgccgc gatcgcccgg
     5221 atcctcccgc tcctcgtcga caaggccacg ccgaacggcc agctgccggc cggcggcatc
     5281 ctcgacgcga tctcgggatt cttcaaagcc tgaggtgaga ggggcggcgg gatgaccgag
     5341 atcaactttc atcccgccgc tccgatcctg cgcgtcgtaa acctcgaggc gagcctcgcc
     5401 tactactcct ccgcgctcgg attcgcgaag gactggggag acaggcggtt cgcctcggtg
     5461 tcacgcggcc gctgcacgct cttcctcgcc gagggcgacc aggggcaccc gggaggatgg
     5521 gtgtacgtcg gcgtcggaga cgccgcggcc ctgcacgacg agctccgcgc gaagggagcg
     5581 aaggtgcgtc agccgccgac gaaccacccc tgggcgctcg agatccaggt cgaggatctc
     5641 gacggcaacg tcctgcgttt cgggagcgac tctctcgaag gacagccctt cggcgagtgg
     5701 ctcgacatgc ggggcgcgcg ctggatcagg tccgcggccg gcgccttcac gcgccgcgag
     5761 gaagagtctt gagtccgatc cgtacgacac tgggcgtttg tgccggggcg tttctcgtcc
     5821 tcagcagcgc cgcccactcc ctgctcggat ggcccgagat ggccgccgcg ctttccaaga
     5881 cgaacgcgcc tgcggaactc gtgaggggac tggccatcgg gtggcacttc ggcggagtcg
     5941 cgatgctcgt cttcggggtc ctcgtgctgc agctcttcct gcgcgcgcgg agggacgact
     6001 ccgtttccct cggccccgct cttctgatcg gcggcgctta cgcggccttc gggctcggtg
     6061 ccctcgcgat cagcggcctg aatccgttct acttcgtctt catcgcgccg ggggcggctc
     6121 tcgttctcgc ctctctcaga aagtcctcga atagacgtag gtgaagccgg tcaccttgca
     6181 ccggcgcacg ggcggttgga acttcacggt cttcacctcg gccttcacgg cttcggcgcg
     6241 ctcgggcgag cccgtctcgt tcgtcacgac gcggacggct ttcacagaac ccgcctcgtc
     6301 gatgtcggcc tcgacgacga ccttgccctt cgcgccgggc gcgtcgctga cgcggagcat
     6361 gggcggctcg atcaggactt ccttgcggtc cgggaggcag ccgtcgtcgc gcttgaggag
     6421 atccttcttc acccagccga tcctcgtgtc ggggagcttc acgcgaaccc agccgtcttt
     6481 ttcttcgaac gaggtcaggc ggatgccttt cgcggccttg gcgacggtcg ccgctttcgt
     6541 ggagggagtc tcccggacgt tcaccgtctt cggcacgatc caggagacct cggcggcctg
     6601 cggcgcgggc gcgggctcgg gagcgggtgc cggctcggga gcgggcggcg gcggcggcgg
     6661 ggccgaggca cagcccca
//
LOCUS       DAOMFY010000223         6672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3761_length_6672_cov_7.363307, whole genome shotgun sequence.
ACCESSION   DAOMFY010000223 DAOMFY010000000
VERSION     DAOMFY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3761_length_6672_cov_7.363307"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1416)
                     /locus_tag="PLB01_15045"
     CDS             complement(91..1416)
                     /locus_tag="PLB01_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ78671.1"
                     /translation="MNLRFLLLAAALPALAAAQPGAPVVSSQAPNLAVRKPTDLASKG
                     MIGAVGDTVPLKATLTSKADGKPLAGKSISFKVAGKDAGNATTGPTGEAKLDYKVPND
                     PPPGPHPMEARFAGDSEWGSSSVSANFGVFKASTKITFNAPPTGVNEGETANVSGKLV
                     RITDQAGIHGREISMTVNGKTAGKVTTSNGGFTLSYPVPKNFPPKATVEAQFEGDVLY
                     AATAATTAFDVKNPVKPGFLLWEGAKGKIGETVTLKARLTEAPVGIGKGISGIVVRFW
                     MDRGPRWAAPHVPQINLCQGTTDSSGGASCSVKLNATALPYTAYAHADVDTQVWNVTK
                     VSDPIIDIDKAAVHLALSGPSTAHIGQSISIKVRVTRTTDGGPVKNASIHLSPAAAKT
                     TDDNGDATFTLPVPSGGTGPKPFKATYAGNDNYHPGEGSITINVTPATN"
     gene            complement(1556..2653)
                     /locus_tag="PLB01_15050"
     CDS             complement(1556..2653)
                     /locus_tag="PLB01_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HQQ78672.1"
                     /translation="MKNLTPAPKKDRTTVIRGSGMYVPPQVVTNEMLGRIMDTTDEWI
                     VPRTGIKERRYVTPGVGSAEIGAKAAEAALLDAGLAASDIDLIVFATMTPDHYFPGNG
                     GLLGTRLGLKTTHALDIRMQCAGFLSGLQTADAFIRSGMYDRVLLVGSEVHSGLMPWP
                     ESVWDVMLGKTTAACDPFWHERATGMRDRAVLFGDGAGAMVLEANTSGDGRGFLGFAM
                     YTDGEHFDKLYVPGGGCKSRPFFTPEMFQNQGTIPIVEGRQVFRLATTLMPAAVQDLL
                     AAHGKTVADLGLLLMHQANLRINEAAQKVLGLPDERVFNNIQKYGNTTAATLPMAYHE
                     AKLAEMVKPGGLVAFTALGAGLHWGAGIAVV"
     gene            complement(2681..2971)
                     /locus_tag="PLB01_15055"
     CDS             complement(2681..2971)
                     /locus_tag="PLB01_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQQ78673.1"
                     /translation="MGPEAPETPVTLPVEDTLDLHTFRPGETAAAVSEYVAAAAARGF
                     REVRLIHGRGIGVQRSIVRRALERSPHVVSFADATPDRGGWGATVAVLGPQR"
     gene            3013..3927
                     /locus_tag="PLB01_15060"
     CDS             3013..3927
                     /locus_tag="PLB01_15060"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HQQ78674.1"
                     /translation="MKRILSAFSLLALLSSPAFAQGNKPRVAILEFKNKASHYTWTWY
                     EAGRGAQDMLVTELVKSGKYRVIEREQLAAIMREKNLSLSGDIDPRTAVKVGKMLGVE
                     YLIAGAVTELGVTDRGANVPGGLLRGLPSVNVRSQKAEAALDARAFSTTTGEIVWADS
                     ARDETSDASVYVFGAGGGANDRGKVDKLMRPVVQKLAASFAKADVKSSGMGGHGDASG
                     LVGKIANVDGGTLYLNAGSEGGVKEGDEFTVYRVGKQIKDPDTGEVLGADETKVGRIR
                     VTAVKGPRLSTASVVSGGGFKAGDTVKN"
     gene            complement(3931..5757)
                     /locus_tag="PLB01_15065"
     CDS             complement(3931..5757)
                     /locus_tag="PLB01_15065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ78675.1"
                     /translation="MPIKTGTVLGRYRMLERAGTGGMSEVWKAEDETLKRTVAVKVIL
                     GPVAQDATFRERFLREARLVAGLEHPNVLPVYDYGSSTVDGEDVSYLVMPLVAGGSLK
                     GRVAGPVPPALAVSWLAAIASALDHAHAKGILHRDIKPANVLMDSQGRPLLADFGLAR
                     STDATSGLTATGTVMGTPLYMAPEQATGGALDGRADEYALAVVAFELLSGRVPFIADS
                     PLAVLHQQVTAPPPPLSTVLPGSPAGADAVLGKALAKKAEERYASCGAFVRALGEALG
                     VPSAAATVPQAASAGGESQLRTVATNDFGAPPLAAPASAPVPGVDTSGRTKWALAAIL
                     LLSAGGGAWLLLRGTGRPGASSEADAKPTPGISLSNKETPAAAAATASTDAGAPSTVE
                     ISDSSKVNEASSASTGTAGPAVEPTPHSPFKEKSERPTPASRIAGNTTRPDAAPVRPR
                     QTAFPPLLSGAGFSGDTQLSAAWEALDTHGRSRLTREDFVNAMGAAREVLARRPTAEV
                     RFLDQYARAGVAYADGRNAEAWQLLTMALRDSGPAADTRVMRFVADEVHAMGPNPGPD
                     SEWVMGLAFGDARGELREELDKAQARAPRSPRVQEARVISGR"
     gene            complement(5782..>6672)
                     /locus_tag="PLB01_15070"
     CDS             complement(5782..>6672)
                     /locus_tag="PLB01_15070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQQ78676.1"
                     /translation="KSVKIGARWGADVGDELARAVPFDNASHPIVRTFLDKTLAIASP
                     AEGAAFFPGNAGGGAAPLIRAAAVPLTYHDELIGVLSVAARRGVDDWPVDLKERLELL
                     ADSAAVALHNARLMRLIEQQTERDSQSGLYNRSSLGKRLESEVRRAERNGQSVAVAHI
                     RMDGLKESVVRLGAEYGDSLLPKLAAKLVRSTRAVNFVARDRDDRFYILIFEAGKVQA
                     HRAMRSIQKNFDNGIDERLVQSGVTLALSAGISVYPDDAFDTATLLARAEDALAEAVK
                     AGPGSVVLYHATEADAAAAG"
BASE COUNT          929 a         2254 c         2497 g          992 t
ORIGIN      
        1 cggccacgct gctctccgcc gagggcctcg acgacgagcg cgacggcgaa gcgtgacggg
       61 cgcccgccgt gaggcgggcg cccgtccgcg tcagttcgtg gcgggcgtca cgttgatcgt
      121 gatcgagccc tcgcccgggt ggtagttgtc gttccccgcg taggtcgcct tgaacggctt
      181 gggcccggtg ccgccgctcg ggaccgggag cgtgaacgtc gcgtcgccgt tgtcgtcggt
      241 cgtcttggcg gcggcggggg acaggtggat cgaagcgttc ttgaccgggc ctccgtcggt
      301 cgtccgcgtc acgcggacct tgatggagat gctctggccg atgtgcgcgg tggacgggcc
      361 ggagagcgcg aggtggaccg ccgccttgtc gatgtcgatg atggggtcgc tgaccttcgt
      421 gacattccag acctgcgtgt cgacgtccgc gtgcgcgtag gccgtgtaag gaagcgccgt
      481 ggcgttcagc ttgacggagc acgacgcgcc tcccgaggag tccgtcgtcc cctggcagag
      541 gttgatctgg gggacgtgcg gagccgccca gcgcggcccg cggtccatcc agaagcggac
      601 gacgatgccc gagatcccct ttccgatgcc cacgggcgcc tcggtgagcc gcgccttgag
      661 cgtcaccgtc tcgccgatct ttcccttggc gccctcccac aggaggaagc cgggcttgac
      721 cgggttcttg acgtcgaacg cggtcgtcgc cgccgtcgcc gcgtagagaa cgtcgccctc
      781 gaactgggct tcgaccgtcg ccttcggggg gaagttcttg gggacgggat aggagagcgt
      841 gaagccgccg ttcgaggtcg tgaccttgcc ggcggtcttg ccgttcacgg tcatcgagat
      901 ctcgcgcccg tggatcccgg cctggtccgt gatccggacg agcttgcccg agacgttcgc
      961 cgtctcgccc tcgttcacgc cggtcggcgg cgcgttgaac gtgatcttcg tcgacgcctt
     1021 gaagacgccg aagttggccg agacggagga ggagccccac tcggagtcgc ccgcgaagcg
     1081 cgcctccatc gggtgcggcc ccggcggcgg gtcgttcggg accttgtagt ccagcttcgc
     1141 ctcgccggtc gggcccgtgg tcgcgttccc ggcgtccttg cccgcgacct tgaaggagat
     1201 cgacttcccc gcgaggggct tgccgtccgc cttcgaggtc agggtcgcct tcaggggcac
     1261 ggtgtcgccg accgcgccga tcatcccctt cgacgcgagg tcggtcggct tgcggacggc
     1321 gaggttcggg gcctggctcg agacgacggg cgcccccggc tgggcagcgg ccagggcggg
     1381 cagggcggcc gcgagaagga ggaaacggag gttcatgtcg gcagcatcct ttcttgtcga
     1441 gatcggagtc tcgccgccaa ggtaggcgct ctgcgcccgc cggtcacgcc aggcgcatgg
     1501 tatgggtgcg aagaaagcag gtctggcggc gccaaacggg cgtggagccg cctcgtcaaa
     1561 ccacggcgat gcccgcaccc cagtggaggc cggcgccgag cgccgtgaag gcgacgaggc
     1621 cgccgggctt caccatctcc gcgagcttcg cctcgtggta cgccatgggc agcgtcgcgg
     1681 cggtcgtgtt gccgtacttc tggatgttgt tgaacacccg ttcgtccggc agcccgagca
     1741 ccttctgagc ggcctcgttg atccgcaggt tcgcctggtg catgaggagg aggccgaggt
     1801 cggcgaccgt cttgccgtgg gccgcgagga gatcctgcac cgccgcgggc atgagggtcg
     1861 tcgcgaggcg gaagacctgg cgcccctcga cgatcggaat cgtcccctgg ttttggaaca
     1921 tttcgggcgt gaagaacggg cgtgacttgc agccgccgcc cggaacgtag agcttgtcga
     1981 agtgctcgcc gtccgtgtac atggcgaagc cgaggaagcc ccggccgtcg cccgacgtgt
     2041 tcgcctcgag gaccatcgcg ccggcgccgt cgccgaaaag aaccgcgcgg tcgcgcatgc
     2101 ccgtcgcccg ctcgtgccag aacgggtcgc acgccgcggt cgtcttcccg agcatgacgt
     2161 cccacacgga ctcgggccac ggcatgaggc cgctgtgaac ctccgacccg acgagaagga
     2221 cgcggtcgta catccccgag cggatgaacg cgtcggccgt ctggagaccc gagaggaacc
     2281 ccgcgcactg catccggatg tcgagcgcgt gcgtcgtctt gaggccgagg cgggtcccga
     2341 gaagccctcc gttgcccggg aagtagtggt cgggcgtcat cgtcgcgaac acgatgaggt
     2401 cgatgtccga agcggcgagg ccggcgtcga ggagcgcggc ctcggcggcc ttcgcgccga
     2461 tctcggcgga gccgactccg ggcgtcacgt accggcgctc cttgatgccg gtccgcggga
     2521 cgatccattc gtcggtcgtg tccatgatcc ggcccagcat ctcgttcgtg acgacctgcg
     2581 gggggacgta catccccgaa cccctgatga cggtcgtgcg gtctttcttg ggcgcaggcg
     2641 tcagattttt cacgcggcgg agtctactgg atgggtcgtg ttaacgctgc gggcccagga
     2701 ccgccacggt cgcgccccag ccgccccggt cgggcgtcgc gtcggcgaac gagacgacgt
     2761 gcggggaccg ctcgagcgcc cgccggacga tcgagcgctg gactccgatc ccgcgcccgt
     2821 ggatcaggcg cacttcccgg aagcccctcg cggcggccgc ggcgacgtac tcggagaccg
     2881 cggccgcggt ctcgcccggc cggaaggtgt gcaggtcgag cgtgtcctcg accgggagcg
     2941 tcaccggcgt ttcgggcgcc tcgggcccca tcgtcctaga atagcgtcct ccgaaggaca
     3001 tgaggtgaca cgatgaagcg aatcctctcc gcgttctccc tgctcgcgct cctctcgtcg
     3061 ccggcgttcg cgcagggcaa caagccgcgc gtggccatcc tcgagttcaa gaacaaggcg
     3121 tcccactaca cgtggacgtg gtacgaggcg gggcgcggcg cgcaggacat gctcgtgacc
     3181 gagctcgtca agtccggcaa gtaccgcgtg atcgagcgcg agcagctcgc cgcgatcatg
     3241 cgcgagaaga atctctcgct ctccggcgac atcgacccgc gcacggccgt gaaggtcggc
     3301 aagatgctcg gcgtcgagta cctgatcgcg ggagccgtga ccgagctcgg cgtcacggac
     3361 cgcggcgcga acgtgcccgg cggcctcctg cgcggcctgc cgtccgtcaa cgttcgctcg
     3421 cagaaggccg aggccgcgct cgacgcgcgc gcgttctcga cgacgacggg cgaaatcgtc
     3481 tgggcggact cggcgcgcga cgagacgtcc gacgcgagcg tctacgtctt cggcgccggc
     3541 ggcggcgcga acgaccgtgg caaggtcgac aagctcatgc ggcccgtcgt ccagaagctc
     3601 gcggcgtcgt tcgcgaaggc cgacgtcaag tcgtccggga tgggtggcca cggcgacgcc
     3661 tccggcctcg tcgggaagat cgcgaacgtg gacggaggca cgctgtacct caacgccggc
     3721 tccgagggcg gcgtgaagga aggcgacgag ttcacggtct accgcgtcgg caagcagatc
     3781 aaggacccgg acaccggcga ggtcctcggc gcggacgaga cgaaggtcgg gcgcatccgg
     3841 gtcacggccg tgaaggggcc gcgcctctcg acggcctcgg tcgtctccgg cggcggcttc
     3901 aaggccggcg ataccgtgaa gaactagcgg ctaacgcccc gaaatcaccc gggcttcctg
     3961 cacgcgcggc gagcggggag cccgggcctg cgctttgtcg agctcttcgc ggagctcgcc
     4021 gcgcgcgtcg ccgaaggcga ggcccatcac ccactcggag tccggaccgg ggttcggccc
     4081 catcgcgtgg acctcgtcgg cgacgaagcg catgacgcgc gtgtcggccg ccgggccgga
     4141 gtcccggagc gccatcgtga ggagctgcca ggcctcggca ttgcggccgt ccgcgtaggc
     4201 gacgccggcg cgagcgtact ggtccaggaa ccgcacctcg gcggtcggcc ggcgcgcgag
     4261 gacctcgcgc gcggcgccca tcgcgttcac gaagtcctcg cgcgtgaggc gcgaacgccc
     4321 gtgcgtgtcg agcgcctccc acgccgcgga gagctgcgtg tcgcccgaga acccggcgcc
     4381 ggagaggagg ggaggaaaag cggtctgccg aggacgtacg ggggcggcgt cggggcgcgt
     4441 cgtgttccct gcgatgcgcg aggccggcgt tggcctttcg gatttctctt tgaagggtga
     4501 gtggggcgtc ggctcgactg ccggcccagc ggtgccggtg gaggcggagc tcgcctcatt
     4561 caccttcgaa gaatccgaaa tctcaaccgt cgagggcgct ccggcatccg tcgaagcggt
     4621 ggcggccgcg gctgccgggg tctctttatt actcaaagaa atcccgggcg ttggttttgc
     4681 gtcggcttcg ctcgacgcgc ccgggcggcc cgtcccgcgg agcagcagcc acgcaccgcc
     4741 ccccgcgctc aggaggagga tcgccgcgag cgcccacttc gtccgtccgc tcgtgtcgac
     4801 cccgggaacc ggcgcggatg cgggggccgc gagcggcggc gcgccgaaat cgttcgtggc
     4861 gaccgtccgg agctgcgact cgccgccggc ggaggccgcc tgcggcacgg tcgccgccgc
     4921 gctcgggacc ccgagcgcct caccgagcgc gcggacgaag gctccgcacg aggcatagcg
     4981 ctcctccgcc tttttcgcga gagccttccc gaggacggcg tcggccccgg ccgggctgcc
     5041 gggaaggacc gtcgagagcg gcgggggcgg cgccgtgacc tgctggtgca ggacggcgag
     5101 cggcgaatcg gcgatgaagg gcacgcggcc cgacaggagc tcgaacgcga cgaccgcgag
     5161 cgcgtactcg tccgcgcggc cgtccagcgc gccgcccgtg gcctgctcgg gcgccatgta
     5221 gagcggcgtc cccatgacgg tccccgtcgc ggtgagcccg gacgtcgcgt ccgtcgagcg
     5281 cgcgagaccg aagtccgcga ggagggggcg gccctgcgag tccatgagga cgttcgcggg
     5341 cttgatgtcg cggtggagga tgcccttggc gtgcgcgtgg tcgagcgcgc tcgcgatcgc
     5401 ggccagccac gagacggcga gggcgggagg gacggggccc gcgacgcggc ccttgagcga
     5461 gccgcccgcg acgaggggca tgacgaggta cgagacgtcc tcgccgtcga ccgtcgagga
     5521 gccgtagtcg tagacgggga ggacgttcgg gtgttcgagg cccgcgacga ggcgggcctc
     5581 gcgcaggaag cgctcgcgga acgtcgcatc ctgcgcgacg ggcccgagga tcaccttgac
     5641 ggcgaccgtg cgcttgagcg tctcgtcctc cgccttccag acctcggaca tcccgcccgt
     5701 gccggcgcgc tcgagcatcc ggtagcggcc caggaccgtt cccgtcttga tcggcatgga
     5761 ggaaggatag cggagggcgg gtcagccggc cgcggcggcg tccgcttcgg tcgcgtggta
     5821 gagcacgacc gagccggggc ccgctttcac ggcctcggcg agcgcgtcct cggcgcgggc
     5881 gaggagcgtc gccgtgtcga acgcgtcgtc ggggtagacc gagatcccgg ccgagagcgc
     5941 gagggtgacg ccggactgca cgaggcgctc gtcgattccg ttgtcgaagt tcttctggat
     6001 cgagcgcatc gcgcggtgcg cctgcacctt gccggcctcg aagatgagga tgtagaaccg
     6061 gtcgtcgcgg tcccgcgcga cgaagttcac ggcgcgcgtc gagcggacga gcttggccgc
     6121 gagcttcggc agcagggagt cgccgtactc ggcgccgagg cgcacgacgg actccttgag
     6181 gccgtccatg cggatgtgcg cgacggcgac ggactgcccg ttgcgctcgg cgcgccgcac
     6241 ctcggactcg aggcgcttgc cgagcgagga gcggttgtag aggcccgact ggctgtcgcg
     6301 ctcggtctgc tgctcgatga ggcgcatgag gcgcgcgttg tggagcgcga cggcggccga
     6361 gtccgcgagg agctccaggc gctccttgag gtccaccggc cagtcgtcga cgccgcggcg
     6421 cgccgcgacg gagaggacgc cgatgagctc gtcgtggtac gtgagcggca cggcggcggc
     6481 ccggatgagc ggcgcggcgc cgcccccggc gttgcccggg aagaacgccg cgccctcggc
     6541 ggggctcgcg atggcgagcg tcttgtcgag gaacgtgcgg acgatcgggt gcgaggcgtt
     6601 gtcgaaggga acggcccgcg cgagctcgtc gccgacgtcg gcgccccagc gcgcgccgat
     6661 cttcacggac tt
//
LOCUS       DAOMFY010000224         6660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3775_length_6660_cov_6.257835, whole genome shotgun sequence.
ACCESSION   DAOMFY010000224 DAOMFY010000000
VERSION     DAOMFY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_6660_cov_6.257835"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PLB01_15075"
     CDS             complement(<1..1347)
                     /locus_tag="PLB01_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQQ78677.1"
                     /translation="MAVPPGKFGDHLTDVYFADLKLPHAVSAGVAACGFVRATPVQAA
                     TLPLLLAGKDVAAQAQTGTGKTAAFLIGIFTRLARTKRPKPSPYPRALAVAPTRELAI
                     QIQEDAKALGHFLKPRIVTVFGGIDYVKQRDQLRAGCDLLIGTPGRLLDYEQQGATSF
                     GEVESLVIDEADRLFDLGFIRDLRRILRRCPPPGKRHSMMFSATLSLRVMELAYEHMN
                     DAVKVEIRPEQVTADRVTQALYHVSQREKMPLLLGLLRREADAAPRGEPLRTLLFVNT
                     KRFAERLVGTLERHGWKIGALTGDIPQARRMKILADFKNGTLPLLVATDVASRGLHIE
                     GVTHVVNVDLPQDPEDYVHRIGRTARAGRTGKAISLADEDYVFSLDAIQKLIGMKIPV
                     EFADDKLFAPPGAAPAAPRKEEKKEEKTAPAAPKISLKVEEKLAPSAPEIPAKVAEP"
     gene            1407..3383
                     /locus_tag="PLB01_15080"
     CDS             1407..3383
                     /locus_tag="PLB01_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQQ78678.1"
                     /translation="MPVRELTKRIFLGKSRDPLDPHVFHQLSLVAFLAWVGLGADGLS
                     SSAYGPEEAFLALGTHTHLAVFLAVATALTVILISASYAQLIELFPSGGGGYLVATHL
                     LGETAGLVSGAALLIDYVLTISTSCVAGVAALSSFFGEISPSRQLAYACVSVVVLVVL
                     NLRGVRESIQVLLPIFLAFLLSHVGILLFGMFRHVGAVPELVAQTAHETRSTWAELGA
                     WGTIMLLLKAFSLGGGTYTGIEAVSNGLQILREPRVKTGKRTMAYMAVSLSFMAGGLI
                     LCYLLANVRKTQGVTLNASLYQILSRELFGAGGVAAAFVLLLLVSEGLLLFVAAQSSF
                     LAGPRVLANMAIDSWVPHRFSLLSERLVTQNGVILMGAAAIAMLVYARGSIHILVLMY
                     SINVFLTFTLSQLGMCVHWWQVRKTERTWGRRFAVNGAGLAISVTILVVSTALKFREG
                     AWMTVVITGALVALCVAIKKHYGSVSLLMKRADDVLTTLPTPKTAPGASAPAPERGAP
                     TAVFLVSGFNGLGIHSLLQVQKYWPRYYRNAVFLSVGVVDSAKFKGTDEMENLRLETQ
                     RDLEKYVEFARGLGMNAEQHYAIGIDLLDEIVDLCKSVKEQYDRPVFFASKLIFPNEN
                     LANRLLHNQTPFAVQRRLQFEGLQTVILPIQVSI"
     gene            3380..5332
                     /locus_tag="PLB01_15085"
     CDS             3380..5332
                     /locus_tag="PLB01_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78679.1"
                     /translation="MKPEARPSPRGPLSSRRPLAFALGLAGLVLAVTTDERVFGLVTD
                     GQIMTRTAYAMAALGEIGVARGHLVGAVRPAGDAVTRYGMGPSLVRVPAAALGGPFEN
                     AFGLGASQTLFVLEQILLVLLAAFSAGLLARALGGDARAVRRAVLGTAIASPLWAYAS
                     SDWSEPLQAALVGGAFSAAAWAVTESPNEEQAPRRAVLLAALAGFLAGFGLLSKSLLV
                     ILFPSVLAALWTPAAGRARRLAAACAGFAPPAALWLALEIVRFGAPFASYGGERFSHP
                     PLDGLWRLTVGPNKGLFVYFPLALLGVWGFVRLARTRRILGLALAGFSAFVLLSTAAW
                     WSWDGTAGWGPRLLVPLVPLLAAAAALAAPALPVAVFGALFTLGVAVNALGALQPDAV
                     LTWYYATVRPRVLTAPEQRAFPAFATQTGADGAVRLLPLHDVANHAALSPLRVNAWLL
                     GRRLAGGDVLAALKTPPWRTDVPGQEAGLPPEQAIPPSALVFLTSPFRWPHLGMSLAR
                     ASGQTDTVLAWIDCLFDQALRAQDMERGERAVAFAEELYRRVPGAQTAATLAEAYRLA
                     GRREALADFVGSLPAAQKASPEFGMVLALAMRDAGNEARARQVLGQVLAASPRPEYAR
                     LANTPLAAWPSTLRKIQFPADAATTP"
     gene            5329..>6660
                     /locus_tag="PLB01_15090"
     CDS             5329..>6660
                     /locus_tag="PLB01_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78680.1"
                     /translation="MKIRRPLAAALLLAGAVLALTADERVYGLMADGRTMLRTAVSIA
                     TLGEIGIARGGAVDVPRPAGDAVSRYGMGPSLVLAVPALLAPRFEKAFGPGASQTLFV
                     LHQISFVLLAALFAGLLARAWGASERAAAAAALATALASPLWAYVALDFSEPLQAALC
                     GGAFAAAAWAVKEPAMEAAPRRVPLLEVLAGFLAGFALLSKSLLVILFVAILAALWAP
                     SVGRARRLAAACAGFVPPAAAWLVFEIVRFGRPFASYGGERFTHAPLDGLWRLLVGAN
                     KGLVFYFPLVLLAAWGAFALLKARRTSSGPAFAFALALLAAAAAWWAWDGTFGWGPRL
                     LMPAIPILAAAAAVAPAPRVVFMALFAAGAAVNGLGALQPSTLTTWTFATLAPRPLAP
                     AEAARYPDFAMRRGPDGASLYPQYFASSEAALAPIPLAARLLAARLSGSGVA"
BASE COUNT          744 a         2464 c         2303 g         1149 t
ORIGIN      
        1 aggctcggcc accttcgcag gaatctccgg cgccgaaggc gcgagcttct cttccacctt
       61 caaagaaatc ttcggcgccg caggcgccgt cttctcttcc ttcttctctt cctttcgcgg
      121 cgccgcaggc gccgcgccgg ggggcgcgaa gagcttgtcg tccgcgaact ccacggggat
      181 cttcatcccg atgagcttct ggatcgcgtc gagcgagaag acgtaatcct cgtccgcgag
      241 cgagatggcc ttgcccgtcc gaccggcgcg cgccgtgcgg ccgatgcggt ggacgtagtc
      301 ctcggggtcc tgcggaagat ccacgttgac gacgtgcgtg acgccctcga tgtggaggcc
      361 gcgcgaggcg acgtccgtcg cgacgaggag cgggagcgtt ccgttcttga agtccgcgag
      421 gatcttcatc cgccgcgcct gcgggatgtc gccggtcagc gcgccgatct tccagccatg
      481 gcgctcgagc gtgccgacga gcctttcggc gaaccgcttc gtgttcacga agagcagcgt
      541 gcggagaggc tctccgcgcg gggccgcatc ggcctcgcgc cggaggagac cgaggaggag
      601 cggcatcttc tcgcgctgcg acacgtggta gagcgcctgc gtgacgcggt cggcggtgac
      661 ctgctccggc cggatctcga ccttcacggc gtcgttcatg tgctcgtagg cgagttccat
      721 cacgcgcagc gagagcgtcg ccgagaacat catcgagtgg cgtttgccgg ggggcgggca
      781 gcggcggagg atgcgccgga ggtcccggat gaagccgagg tcgaaaaggc ggtcggcctc
      841 gtcgatcacg agggactcca cctcgccgaa ggacgtcgcg ccctgctgct cgtagtcgag
      901 gaggcggccc ggcgttccga tcaggagatc gcagcccgcc cgaagctggt cgcgctgctt
      961 gacgtagtcg atccctccga agacggtgac gatcctcggc ttgaggaagt ggccgagggc
     1021 cttcgcgtcc tcctggatct ggatcgcgag ctcgcgcgtg ggcgcgaccg cgagggcgcg
     1081 cgggtacggc gagggcttcg ggcgtttcgt gcgcgcgagg cgcgtgaaga tcccgatcag
     1141 gaaggcggcc gtcttcccgg tgcccgtctg cgcctgcgcg gcaacgtcct tgccggcgag
     1201 gaggaggggc agcgtcgcgg cctgcacggg ggtcgcgcgc acgaatccgc acgcggcgac
     1261 gcccgcgctc acggcatggg ggagcttgag gtcggcgaag tagacgtcgg tgaggtgatc
     1321 gccgaatttt cccgggggga cggccatcca gccgtcattg taacgtccgc ggcttcccgg
     1381 aacgtccgcg tatactcgat cgagtcgtgc ctgttcggga gctgacgaaa cggatcttcc
     1441 tcggcaagag ccgggatcct cttgatccgc acgtcttcca tcagctgtcc ctggtggcct
     1501 ttctcgcgtg ggtgggcctc ggggcggacg ggttgtcgtc ctcggcgtac ggcccggaag
     1561 aagcgttcct cgctctcggg acgcacaccc acctcgccgt cttcctcgcc gtggcgaccg
     1621 cgctcacggt catcctgatc tcggcgtcct acgcccagct catagagttg ttcccgtctg
     1681 gaggaggcgg ctacctcgtc gcgacccacc tgctcggcga gacggccggg ctcgtctccg
     1741 gcgccgccct cctcatcgac tacgtcctga cgatttccac gtcctgcgtg gcgggcgtcg
     1801 ccgccctctc ctcgttcttc ggcgagatct ccccgtcccg ccagctcgcg tacgcctgcg
     1861 tctccgtcgt cgtcctcgtg gtcctgaacc tgcgcggcgt gcgcgagtcg atccaggtgc
     1921 tgctcccgat cttcctcgcg ttcctgctct cgcacgtcgg gatcctgctc ttcgggatgt
     1981 tccgccacgt cggggcggtt cccgagctcg tcgcccagac ggcccacgag acgcggtcca
     2041 catgggccga gctcggcgcg tggggcacga tcatgctgct cctcaaggcg ttctccctcg
     2101 gcggcggcac gtacaccgga atcgaggcgg tctcgaacgg gctccagatc ctgcgcgagc
     2161 cgcgcgtgaa gaccggcaag cgcacgatgg cgtacatggc ggtctccctc tcgttcatgg
     2221 cgggagggct gatcctctgc tacctcctcg cgaacgtgcg gaagacccag ggcgtgacgc
     2281 tgaacgcgtc gctctaccag atcctctccc gcgagctgtt cggcgcgggc ggcgttgccg
     2341 ccgcgttcgt cctgctcctc ctcgtctcgg agggcctcct cctcttcgtc gcggcgcagt
     2401 cgagcttcct cgcgggaccg cgcgtcctcg cgaacatggc gatcgactcc tgggtgccgc
     2461 accgcttcag cctgctctcg gagcggctcg tgacgcagaa cggcgtgatc ctcatgggcg
     2521 cggccgcgat cgcgatgctc gtctacgcgc gcggttcgat ccacatcctc gtcctgatgt
     2581 actcgatcaa cgtcttcctg acgttcacgc tgtcccagct cgggatgtgc gtgcactggt
     2641 ggcaggtgcg gaagacggag cgcacgtggg gccggcgctt cgccgtgaac ggcgcggggc
     2701 tcgccatctc ggtgacgatt ctcgtcgtct ccacggccct gaagttccgc gaaggcgcgt
     2761 ggatgaccgt cgtcatcacg ggagcgctcg tcgcgctctg cgtcgccatc aagaagcact
     2821 acgggtcggt gagccttctc atgaagcgcg ccgacgacgt cctcacgacg ctcccgacgc
     2881 cgaagacggc ccccggcgcg tccgcgcccg cgccggaacg cggggcgccg acggccgtct
     2941 tcctcgtctc gggcttcaac ggcctcggca tccactcgct cctccaggtg cagaagtact
     3001 ggccccggta ctaccgcaac gccgtcttcc tctccgtcgg cgtcgtggac tcggcgaagt
     3061 tcaagggcac ggacgagatg gagaacctgc gcctcgagac gcagcgcgac ctcgagaagt
     3121 acgtcgagtt cgcccgcggg ctcgggatga acgcggagca gcactacgcg atcgggatcg
     3181 accttctcga cgagatcgtc gacctctgca agagcgtcaa ggagcagtac gacaggcccg
     3241 tcttcttcgc gtcgaagctc atcttcccga acgagaacct cgcgaaccgg ctgctccaca
     3301 accagactcc gttcgccgtc cagcggcgcc tccagttcga gggcctgcag acggtgatcc
     3361 tgccgatcca ggtctcgatt tgaagccgga ggcgcggccg tccccccggg ggcctctctc
     3421 gtcgcgccgc ccgctcgcct tcgccctcgg cctcgccggc ctcgtcctcg ccgtcacgac
     3481 ggacgagcgc gtcttcggcc tcgtcacgga cggccagatc atgacccgca cggcatacgc
     3541 gatggccgcg ctcggcgaga tcggcgtggc ccgcggccac ctcgtcggcg ccgtgcggcc
     3601 cgcgggcgac gcggtgaccc gctacggcat ggggccgtcg ctcgtgcggg tgccggcggc
     3661 ggcgctcggc ggcccgttcg agaacgcgtt cggactcggc gcctcgcaga cgctgttcgt
     3721 cctcgagcag atcctcctcg tcctgctcgc ggcgttctcc gccggactcc tggcccgtgc
     3781 gctcgggggg gacgcccgcg ccgtgcgtcg ggcggtcctc ggcaccgcga tcgcgtcgcc
     3841 gctctgggcc tacgcctcgt cggactggtc cgagcccctc caggctgcct tggtgggagg
     3901 tgcgttctcc gcagcggcct gggccgtcac ggagtctccg aacgaggagc aggcaccgcg
     3961 gcgtgcggtt ctcctcgccg cgctcgccgg attcctcgcc ggattcggac ttctttcgaa
     4021 gagtctcctg gtcattctct tcccctcagt cctcgccgca ctgtggactc ccgcggcggg
     4081 ccgcgcgcgc cggctcgcgg cagcctgcgc gggctttgca ccgcccgccg cgctgtggct
     4141 cgcgctggag atcgtgcgct tcggcgcgcc gttcgcctcg tacggcgggg aacgcttcag
     4201 ccacccgccg ctcgacggcc tgtggcggct gacggtcggg ccgaacaagg gcctcttcgt
     4261 ctactttccg ctcgcgctcc tgggcgtctg gggcttcgtc cgcctcgcgc ggacgcgccg
     4321 catcctcggg ctcgcgctcg ccgggttctc ggcgttcgtc ctcctctcga cggcggcgtg
     4381 gtggtcgtgg gacggaaccg cgggctgggg cccgcggctc ctcgtcccgc tcgttccgct
     4441 cctcgcggcg gcggcggccc tcgcggctcc ggcccttccc gtggcggtgt tcggcgcgct
     4501 cttcacgctc ggcgtcgccg tgaacgccct cggcgcgctc cagcccgacg cggtcctgac
     4561 ctggtactac gcgacggtca ggccgcgcgt cctcacggcg ccggagcagc gcgcgttccc
     4621 ggcgttcgcc acccagaccg gcgcggacgg cgccgtgcgg ctccttccgc tccacgacgt
     4681 cgcgaaccac gccgcgctgt cgcctcttcg cgtgaacgcc tggctcctcg gccggcgcct
     4741 cgcgggcgga gacgttctcg ccgcgttgaa gaccccgccg tggcgcacgg acgttcccgg
     4801 acaggaggcg ggcctcccgc ccgagcaggc gattccgccc tccgcgctcg tcttcctgac
     4861 gtcgccgttc cgctggccgc acctcgggat gtcgctcgcg cgcgcatccg ggcagacgga
     4921 cacggtgctc gcgtggatcg actgcctgtt cgaccaggcg ctccgcgcgc aggacatgga
     4981 gcgcggcgag cgggccgttg cgttcgccga ggagctctac cggcgcgtgc cgggcgcgca
     5041 gaccgccgcg acgctcgcgg aggcgtatcg gctggcgggg cggcgcgagg ctctcgcgga
     5101 cttcgtcgga tcgctgcccg ccgcgcagaa ggcgtcgccg gagttcggga tggtcctcgc
     5161 cctcgcgatg cgcgacgcgg gcaacgaagc gcgggctcgc caggtgctcg ggcaggtcct
     5221 cgcggcgagc ccgcggcccg agtacgcgcg gctcgcgaac acgccgctcg ccgcatggcc
     5281 gtccacgctg cggaagatcc agttcccggc cgacgccgcg acgacgccgt gaagatccgg
     5341 cgcccgctcg ccgccgcgct cctcctcgcg ggcgccgtcc tcgcgctcac cgccgacgag
     5401 cgcgtctacg ggctcatggc ggacggccgg acgatgctcc gcaccgccgt gtcgatcgcg
     5461 acgctcgggg agatcgggat cgcgcgcggg ggcgccgtgg acgtcccgcg gcctgcgggc
     5521 gacgccgtgt cccgctacgg aatgggtcct tctctcgtcc tggccgtgcc ggccctcctc
     5581 gccccccgct tcgagaaggc gttcggaccg ggggcgtcgc agaccctttt cgtgctgcac
     5641 cagatctcgt tcgtcctcct cgcggccctc ttcgcgggtc tcctcgcgcg cgcgtggggc
     5701 gcttcggagc gcgccgcggc cgcggccgcc ctggcaaccg ctctcgcctc gccgctctgg
     5761 gcctatgtcg ccctcgattt ctcggagcct ctgcaggccg cgctgtgcgg cggggcgttc
     5821 gcggcggcgg cgtgggccgt gaaggaaccc gcaatggagg ccgccccgcg acgcgtgcct
     5881 ctcctcgagg ttctcgccgg gttcttggcc ggattcgccc ttctttcgaa gagtctcctg
     5941 gtgattctct tcgtcgcgat cctcgcggcg ttgtgggctc ccagcgtggg ccgcgcgcgc
     6001 cggctcgcgg ccgcgtgcgc gggcttcgtg ccgccggcgg ccgcgtggct cgtcttcgag
     6061 atcgtccggt tcgggcgccc gttcgcctcc tacgggggcg agcggttcac gcacgctccg
     6121 ctcgacgggc tgtggcgcct cctcgtcggc gcgaacaagg gcctcgtctt ctacttcccg
     6181 ctcgtcctcc tcgccgcgtg gggcgcgttc gcgctcctga aggcgcggcg cacctcgtcc
     6241 gggccggcct tcgcgttcgc cctcgcgctc cttgcggcgg ccgccgcgtg gtgggcgtgg
     6301 gacgggacgt tcgggtgggg gccgcgcctc ctgatgccgg cgattccgat cctcgccgcc
     6361 gcggcggccg tcgcgcccgc gccgcgcgtc gtcttcatgg cgctcttcgc cgccggagcc
     6421 gcggtgaacg gcctcggggc gcttcagccc tcgacgctca cgacgtggac gttcgcgacg
     6481 ctcgctccga ggcccctcgc ccccgccgag gccgcgcgct atccggactt cgcgatgcgg
     6541 cgcgggccgg acggcgcgtc cctctacccg cagtacttcg cgtcctccga ggcggcgctc
     6601 gcgccgatcc cgctcgcggc ccggctcctc gcggcgcggc tgtccggctc cggcgtcgcc
//
LOCUS       DAOMFY010000225         6642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3792_length_6642_cov_5.557158, whole genome shotgun sequence.
ACCESSION   DAOMFY010000225 DAOMFY010000000
VERSION     DAOMFY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3792_length_6642_cov_5.557158"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLB01_15095"
     CDS             complement(<1..522)
                     /locus_tag="PLB01_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78681.1"
                     /translation="MPAGLVLAAALFTGSAPAAAPVLPVLVSYDVVEATRTVTPAGER
                     NGGTAGSVRVSAGRARWELPHGTFPRSSASVAISDGPVVTLLDPKERLAAAATADDFT
                     ALFRGRPATEGSAATALRDVSVRLTPGGKGRAFDGRPTSRFLLDAGWTLVVTTTGRVS
                     RVTTEIKGVVETVD"
     gene            584..2299
                     /locus_tag="PLB01_15100"
     CDS             584..2299
                     /locus_tag="PLB01_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ78682.1"
                     /translation="MEPKADAMARPLRDGVLRTVRLVGEPEAMDWVARTLAGSGLLVE
                     RHDERDVHDPKFFLHWPVVWVTRPEAFGRGFALARPPLLDVAKAPPPCLVVVPPGVEI
                     TDPLVRRIPVESFEVVRERDEAPLLAMRLERLLLLHRRRSASEENAFKERLRVEAKRN
                     EILASIALAARDSLDLEEILDAATELLGTRFSANSVEIWFLNDDGESCTVLMDWRPGD
                     VPTSFVGYERPLPDSEPFRALVTSTDPYVVADRGELAQTDPLAAEALEALGAESFLGI
                     PIHREGEAIGVLGMSWESPRAFPQDELVFFGRVADQLALAIRAARLYGNLQSQVEELA
                     LEQRRRALADRDRSRLTAMLVHDMKNPLSAISAALELTRDKERRAGDERLAKLLDGSL
                     ASARGLQGLIEDALLVYRPGDAPETERRPASVAEALALPLEEARWMAVPRGVAVAVDV
                     PASLPHVRLDSKMFRRAAANLLGNAVKFSPKGGTVRIAARVVDEDGKAFLRLDVSDEG
                     PGFPPAEKSRLATPYLRFQGSETVPGTGLGLTVVQKVLQAHRGRLDVANNDGPGATFT
                     LWIPA"
     gene            complement(2301..2786)
                     /locus_tag="PLB01_15105"
     CDS             complement(2301..2786)
                     /locus_tag="PLB01_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78683.1"
                     /translation="MTAPDPDLPMLRHTVATLAYRAGKVLRDAPPAFGSFRAGEGGKT
                     AVEILAHMGDLFDWALTMADGKAVWHDAPPLPWSEEVTRFFAALERFDARLATGAPLA
                     QPAPKIFQGPVADALTHTGQLAMLRRMSGAKMKGENYFVADVAAGRVGLAQSPPKREF
                     D"
     gene            complement(2783..3817)
                     /locus_tag="PLB01_15110"
     CDS             complement(2783..3817)
                     /locus_tag="PLB01_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQQ78684.1"
                     /translation="MKALFLAAHGGRDVLQYGDRPDPAAGPGEILVRVRAAALNRLDL
                     FVLAGIPGVPIALPHVPGADGCGVVEALGAGVKGPAPGTRVVIQPGLSCGACEFCRAG
                     QQSLCISFRILGEHVPGTFAELVSVPAVNVSPAPAGLTDEQAAAFPLAALTAWRLLVT
                     RAALRPGETVLIHGIGGGVSTFALQIAKLCGASRVIVTSSSEEKRARAKDMGADDVLD
                     SREDVGKRVRELTKKRGVDVVVDSVGAATWKHSLVAAAKGGRIVTPGATSGPNPEEEI
                     RHIFWKQLSILGSTMGTDAEFASLLAAVSAGRIVPVVDSVVPLAEGRKAYERMQSSQG
                     LGKIVVEVAG"
     gene            3898..4215
                     /locus_tag="PLB01_15115"
     CDS             3898..4215
                     /locus_tag="PLB01_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78685.1"
                     /translation="MRAWEIRQKRAQKRARELARKEKNKHAKETSTFRLVVAGREPAV
                     VESVIVKNPDESVLEATFDEIETGMTLIVEGDRGLIQRAAVEEKVEITEPELVREVRV
                     RIL"
     gene            4267..5732
                     /locus_tag="PLB01_15120"
                     /pseudo
     CDS             4267..5732
                     /locus_tag="PLB01_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558474.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            5729..6211
                     /locus_tag="PLB01_15125"
     CDS             5729..6211
                     /locus_tag="PLB01_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQQ78686.1"
                     /translation="MSAAAGRRHRLVRTQSIPRHREAVFGFFADASNLEEITPPFLRF
                     RILTPAPVEMRTGARIDYALSLFGIPLRWRTRITEWEPGVRFVDEQESGPYAVWRHAH
                     EFESDGATTLVRDVVDYSLPMGPLGSLAHVLFVERTLGRIFDFRREAIRRRFGPGESA
                     "
     gene            6244..>6642
                     /locus_tag="PLB01_15130"
     CDS             6244..>6642
                     /locus_tag="PLB01_15130"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HQQ78687.1"
                     /translation="MPARKRPVSARPDRPDWGPLLVALLAGDESAVQDAFERARAHRM
                     DLAAVARDLVEPALEQIGVMWQRGEISIGEEHLATALVARAFQLGARDVPLPGLGAPR
                     LVLVCPAGEFHDLGARIVAEIGRQEGWQVEF"
BASE COUNT          900 a         2443 c         2394 g          905 t
ORIGIN      
        1 gtcgacggtt tcgacgacgc ccttgatctc cgtcgtcacg cgggagacgc gcccggtcgt
       61 cgtcacgacg agcgtccagc ccgcgtcgag gaggaacctc gacgtcggcc ggccgtcgaa
      121 agcgcgcccc ttcccgcccg gggtcagccg gaccgagacg tcgcgcagcg ccgtcgcggc
      181 gctgccctcc gtcgcgggcc gcccgcggaa cagcgccgtg aagtcgtccg cggtcgcggc
      241 ggccgcgagc cgctccttcg ggtcgaggag cgtcacgacg ggaccgtcgg agatggccac
      301 ggacgcgctc gagcgcggga acgtcccgtg cggaagttcc cagcgcgccc gcccggccga
      361 gacgcgcacg gagcccgcgg tgccgccgtt cctctcgccc gcgggcgtga cggtgcgcgt
      421 ggcctcgacg acgtcgtagg agacgagaac gggcaggacc ggggcggccg cgggcgcgga
      481 gccggtgaag agagcggcgg cgaggacgag gccggccggc acggagcgat catcgcacgg
      541 ggaccggtgg acgccgggtc gcgcccgcgg cgagaatgcg tccgtggagc ccaaggccga
      601 cgcgatggcg cgcccgctgc gcgacggggt tctgaggacc gtccggctcg tgggagaacc
      661 cgaggcgatg gactgggtcg cgcgcacgct ggccggctcc ggcctcctcg tcgagcggca
      721 cgacgaacgt gacgtccacg acccgaagtt cttccttcac tggccggtcg tgtgggtcac
      781 gcgccccgaa gcgttcgggc gcggcttcgc gctcgcccgc ccgccgctcc tcgacgtcgc
      841 gaaggcgccg ccgccgtgcc tcgtcgtcgt gccgcccggc gtcgagatca cggatcctct
      901 cgtgcgccgc atccccgtcg agtcgttcga ggtcgtgcgc gagcgcgacg aggccccgct
      961 gctcgcgatg cgcctcgagc gcctgctgct cctccaccgg cggcgctcgg cgagcgagga
     1021 gaacgcgttc aaggagcgcc tgcgcgtcga ggcgaagcgc aacgagatcc tcgcctcgat
     1081 cgcgctggcc gcgcgcgact cgctcgacct cgaggagatc cttgacgcgg cgaccgagct
     1141 cctcggcacg cgcttcagcg cgaactccgt cgaaatctgg ttcctgaacg acgacggcga
     1201 gtcgtgcacg gtcctgatgg actggaggcc cggcgacgtc ccgacctcgt tcgtcggata
     1261 cgagcggccc ctgcccgaca gcgagccgtt ccgcgccctg gtgacgtcga cggacccgta
     1321 cgtggtcgcc gaccgcggcg agctggccca gaccgatccg ctcgcggcgg aggccctcga
     1381 ggcgctcggg gccgagagtt tcctcggcat cccgatccac cgcgagggcg aggcgatcgg
     1441 cgtcctcggg atgtcgtggg agtctccgcg cgcgttcccg caggacgagc tcgtcttctt
     1501 tggccgcgtc gcggaccagc tcgcgctcgc gattcgcgcc gcgcggctct acggcaacct
     1561 gcagagccag gtcgaggagc tcgcgctcga gcagcgccgg cgcgcgctcg ccgaccggga
     1621 ccgctcgcgg ctgacggcga tgctcgtgca cgacatgaag aacccgctct cggcgatctc
     1681 ggccgcgctc gagctcacgc gcgacaagga acgccgcgcg ggcgacgagc ggctcgcgaa
     1741 gctgctcgac ggctcgctcg cttcggcgcg cggcctgcag ggtctcatcg aggacgcgct
     1801 cctcgtctac cgccccgggg acgcgcccga aaccgagagg aggcccgcgt ccgtcgccga
     1861 ggcgctcgcg ctgcctctcg aggaggcgcg gtggatggcc gtcccgcgcg gcgtggcggt
     1921 tgccgtggac gtccccgcga gcctcccgca cgtgcgcctg gactcgaaga tgttccggcg
     1981 cgcagcggcg aacctgctgg gcaacgccgt gaagttcagc ccgaagggcg ggaccgtccg
     2041 catcgcggcg cgcgtcgtgg acgaggacgg aaaggcgttc ctccgcctcg acgtctcgga
     2101 cgaaggcccg ggcttccctc ccgcggagaa gtcccgactc gcgacgccct acctgagatt
     2161 ccaggggtcc gagaccgtgc ccggcacggg cctcggcctc acggtcgtgc agaaggtgct
     2221 gcaggcgcac cgcgggcggc tggacgtcgc gaacaacgac ggccccggcg cgacgttcac
     2281 gctctggatc ccggcatagg tcagtcgaac tcgcgcttcg gcggcgactg cgcgagcccg
     2341 acgcggccgg cggcgacgtc ggcgacgaag tagttctcgc ccttcatctt cgcccccgac
     2401 atccggcgca gcatcgcgag ctgccccgtg tgcgtgagcg cgtccgcgac ggggccctgg
     2461 aagatcttcg gcgcgggctg cgcgagcggc gctccggtcg cgagccgggc gtcgaagcgc
     2521 tcgagcgccg cgaagaagcg cgtgacctcc tcgctccacg ggagaggcgg cgcgtcgtgc
     2581 cagacggcct tcccgtccgc catcgtgagc gcccagtcga agaggtctcc catgtgcgcg
     2641 aggatctcga ccgccgtctt cccgccctcc cccgcccgga aagagccgaa cgcgggcggc
     2701 gcgtcgcgca gcaccttgcc cgcgcggtac gcgagcgtcg cgacggtgtg gcgcaacatc
     2761 gggagatcgg ggtccggcgc ggtcatccgg ccacctccac gacgatcttg ccgaggccct
     2821 gggacgactg catccgctcg taagccttcc gcccctcggc gagcgggacg accgagtcca
     2881 ccaccggaac gatccggccc gcggagacgg cggcgaggag ggacgcgaac tcggcgtcgg
     2941 tccccatcgt cgagccgagg atcgagagct gcttccagaa gatgtgccgg atctcctcct
     3001 ccgggttcgg gcccgaggtc gcgccggggg tcacgatccg gccgcccttg gccgccgcga
     3061 cgagggagtg tttccacgtg gccgcaccga ccgagtcgac gacgacgtcg acgccgcgct
     3121 tcttcgtcag ctccctcacg cgcttgccga cgtcctccct cgagtcgagg acgtcgtcgg
     3181 cgcccatgtc ctttgcgcgg gcgcgcttct cctcggacga cgacgtgacg atcacgcggg
     3241 aggcgccgca gagcttcgcg atctggagcg caaacgtgga gacgccgccg ccgatgccgt
     3301 ggatgaggac cgtctcgccc ggcctcagcg ccgcgcgcgt cacgagaagg cgccaggccg
     3361 tgagcgcggc gagcgggaac gccgccgcct gctcgtccgt gaggccggcg ggcgccgggg
     3421 agacgttcac ggccggcacg gagacgagct ccgcgaaggt gccggggacg tgctcgccga
     3481 ggatccggaa cgagatgcac aggctctgct ggcccgcccg gcagaactcg caggcgccgc
     3541 acgagagccc gggctggatc acgacgcgcg tgcccggcgc gggccccttg acgccggcgc
     3601 cgagcgcctc gacgacgccg cagccgtccg cgcccgggac gtgcggcagg gcgatcggga
     3661 cgccggggat cccggccagg acgaagaggt cgagccggtt cagggcggcg gcgcggacgc
     3721 gcacgaggat ctcgccggga ccggccgcgg gatcgggccg gtcgccgtac tggagcacgt
     3781 cgcggccgcc gtgcgcggcg aggaagaggg ccttcatcga tgcctccgaa gcggacgata
     3841 ccgcccggcg ggtatcatgc ccgcggacgg cgccacgggc gccccggaag gagccgaatg
     3901 cgggcctggg aaatcagaca gaagagagcg cagaagaggg cgcgcgagct cgcgcgcaag
     3961 gaaaagaaca agcacgccaa ggaaacgtcc acgttccgcc tcgtcgtcgc gggccgcgag
     4021 ccagccgtcg tggagtccgt catcgtgaag aacccggacg agtccgtcct ggaggccacg
     4081 ttcgacgaga tcgagacggg tatgacgctc atcgtcgagg gcgaccgcgg gctcatccag
     4141 cgcgccgccg tcgaggaaaa ggtcgagatc acggagcccg agctggtccg cgaggtgcgg
     4201 gtccggatcc tctagctccg cgacatccca ttccggcgcc ggtgcgtagg ggctaccgga
     4261 gactccatga acacgccgac ggcccgacgc ctgatcctgc tgaccggcgc cacgggttac
     4321 gtgggcggga ggctgcacgc ggagctcgag aggcggggcg aggcccttcg atgcttcgtc
     4381 cgccggccgg aggagctgcg cggccgggcg gccgcctcga ccgagctcgc cgccggagac
     4441 gcgctcgacg ccccggccgt tgcgcgcgcc ctcgcgggcg tcgacgtcgc ctactacctg
     4501 atccactcga tgggcggcga ggatttcgcc gcgcgcgacc gggaggccgc ccgcatcttc
     4561 ggcaccgccg cacgggaagc cggcgtcagg cgcatcgtct acctgggcgg tctgggcgag
     4621 gccgggggcg ggctctccga gcatctcctg agccgccacg aaacgggcgc gatcctgcgg
     4681 gagtcgagct cagggcctcg atcgtgatcg gctccggaag cctctccttc gagatgatcc
     4741 gcgcgctggt cgagcggctt cccgtgatga tctgcccacg ctgggtcggg gtggaagccc
     4801 agcccatcgc cgtcgaggac gtgatcgcct tcctggctgc ggcgctcgat ctcccgcaag
     4861 gcgccgagcg cgtctacgaa atcggcggcc ccgatcgcgt gacctacgga gacctgatgc
     4921 gcgagtacgc gcgccagcgc ggcctcaagc ggctcctgat tcccgttccg ctcctgacac
     4981 cccggctgtc gagcctctgg ctcggcctcg tgacgccgct ctacgcccgc gtcggacgca
     5041 agctcgtgga cagcatgcgc aatcccaccg tcgtccgcga cttcgccgcc gccgccgcct
     5101 tccccgtcag gccgcgcggc gtcaccgagg cgatcgggcg ggctctcgtg aacgaggacg
     5161 acgcgttcgc ccggacgcgc tggtcggacg ccgtctccgc ctccgggctg agcgagcgct
     5221 acggaggcga ccggatcggc acgcgcttcg tcgacagccg gaaggccgtc gtcggggtgc
     5281 ccccttccgc ggcattcgcc ccgatccggc gcatcggagg cgacaccggc tggtactacg
     5341 gcaacgccct ctggaggctc cgagggttcc tcgacctgct cgccggaggc gtcggcctgc
     5401 gccgcggccg ccgccatccg gacgagctcc gggagggcga cgctctcgac ttctggcgcg
     5461 tcgacgccgt cgagccgaac cgcctgctgc gcctgcgggc cgagatgagg gttccgggcc
     5521 gggcctggct gcagttcgag gtcacgcccg ttgcggacgg ctccgagatc cgccagaccg
     5581 ccgtcttcga cgcctccggt ctcctcggcc tcgcgtactg gtacgccctc tatccgcttc
     5641 atcgcttcgt cttcggcggc atgctccgcg gcatcgcggc ctccgcgctc ggctccccgc
     5701 agaccccgac gtctccgcgg aaggcggcat gagcgcggcc gcgggacgca ggcaccggct
     5761 cgtgcgcacg cagagcatcc ctcggcaccg ggaggccgtt ttcgggttct tcgccgacgc
     5821 atcgaacctg gaagagatca cgccgccctt ccttcgcttc cgcatcctca cgccggcgcc
     5881 cgtcgagatg cgcacgggag cgcgcatcga ttacgcgctc tccctcttcg gaatcccgct
     5941 ccggtggcgc acccggatca ccgagtggga gcccggcgtg cgcttcgtgg acgagcagga
     6001 atccggcccc tacgccgtct ggcgccacgc ccacgagttc gagtccgacg gggcgacgac
     6061 gctcgtgcgc gacgtcgtcg actattccct gccgatggga ccgctcgggt cgctggccca
     6121 cgtcctcttc gtcgaacgca ccctcggccg gatcttcgac ttccggcgcg aggcgatccg
     6181 gcggcggttc ggccccggag aatccgcgtg aagatgcgag gcgtatccct tatcgtgcct
     6241 tccgtgcctg cccgaaaacg cccggtttca gcccgcccgg accggcccga ctggggtccc
     6301 cttctcgtgg cgctcctcgc gggcgacgag tcggccgtgc aggacgcctt cgagcgcgcc
     6361 cgcgcgcacc ggatggatct cgcggccgtg gcgcgggacc tcgtcgagcc ggccctcgag
     6421 cagatcgggg tgatgtggca gcgcggcgag atttccatcg gagaagagca cctcgccacg
     6481 gcgctcgtcg cgcgcgcctt ccagctcggc gcgcgggacg tccccctgcc cggcctcggc
     6541 gcgccccgcc tcgtgctcgt ctgtcccgcc ggggagttcc acgacctcgg ggcgcgcatc
     6601 gttgccgaga tcgggcgcca ggaagggtgg caggtcgagt tc
//
LOCUS       DAOMFY010000226         6601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3824_length_6601_cov_6.445005, whole genome shotgun sequence.
ACCESSION   DAOMFY010000226 DAOMFY010000000
VERSION     DAOMFY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_6601_cov_6.445005"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1406
                     /gene="glmM"
                     /locus_tag="PLB01_15135"
     CDS             93..1406
                     /gene="glmM"
                     /locus_tag="PLB01_15135"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQQ78688.1"
                     /translation="MRRLFGTDGIRGKAGEPPLDAATVSRVGAALAASLGHANPAVVV
                     GCDTRESSGAIVWALTGGLTKAGGLVRFAGVVPTPAVAFLAKELGADAGVVVSASHNP
                     WTDNGVKIFSGEGRKLPDDVELEIERHIDEAKPAPMAPAVVEPDLGRAYVDHLVATLP
                     HRLDGLRVVIDAANGAAFEAAPAAFAAAGATVIARNASPDGRNINEGCGALHPGAMRD
                     AVRAEGADLGIALDGDADRIIVADDAGTLLDGDDVLFLWTLEMEREGKRPASVVGTVM
                     SNWGLETALAGRGVALVRAAVGDRYVVEEMEKSGALLGGEQSGHLIRADLTTTGDGTL
                     TGLHLAALVAASGMKLSAQPRFVHTPQVLKNVKVKARVPFEEIPGFAPLWRAAEAKMS
                     GAGRILLRYSGTEALARVMVEGRDAALVDTVASDLAAHIGARLGA"
     gene            1411..2832
                     /locus_tag="PLB01_15140"
     CDS             1411..2832
                     /locus_tag="PLB01_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HQQ78689.1"
                     /translation="MEFPLEPSEEQLRTLLDDAVRRVVAHVASLPAQPADGTTGAGDL
                     ARALIERDPPEQGGAAGPLLDLLFDRAIPASFNNAGPGFLAYIPGGGQPYAAAADLIS
                     ASVNRYTGVFAPAPALVQLETNVIRWFARIVGYPGGAGGFLTSGGSLANFSAVVAARH
                     EKLPENFLKGTLYVSDQGHHCIRKAALLAGFPDASVREIPSDAAFRMRLDALAARVAA
                     DRAAGFTPFLVVASAGTTNTGAVDDLDALANFAAHEGLWLHTDAAYGGFFALTERGRK
                     ALHGLSRADSIVLDPHKGLFMPYGSGCLLVKDPGALRRSHSVQAHYMPGMQEDPDSVD
                     FCEISPELSRNFRGLPVWLAIKLCGLAAFRASLDEKLDLARDAVAAVRAIPGIAIAAE
                     PQLSLFAFRLAPPGASLEESNRLSRELMARVNARRRVLLTGTLLGEEFAVRMCILSFR
                     THRDRIEMALDDLRDAAGALLRE"
     gene            2964..3389
                     /gene="msrB"
                     /locus_tag="PLB01_15145"
     CDS             2964..3389
                     /gene="msrB"
                     /locus_tag="PLB01_15145"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQQ78690.1"
                     /translation="MTAPASPAASQDRVIKTDEEWKKLLTPAQYNVTRKKGTERAFTG
                     PNWDNHAKGTYVCVCCDLPLFDSETKFDSGTGWPSFWQPIAPGAVRTEEDRSFFSVRT
                     EVLCRRCDAHLGHVFDDGPKPTGLRYCMNGTAMKFIPAK"
     gene            complement(3373..4410)
                     /locus_tag="PLB01_15150"
     CDS             complement(3373..4410)
                     /locus_tag="PLB01_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78691.1"
                     /translation="MSVDPGPAFVDDLFARLLIDDAWALRDERSFSWWPHRVVQRVHA
                     EEPVGEGEAAASRVHVETDVLFADAVTLRQAGVLADLLRYPPLASFVLDREDGVVRLW
                     SAALVTRETAPVALGFLSASAALQAIYAEGGREGLEDELGLPAAASEHPKSGSRPHPD
                     GMLDLLSARIAPEGKHLSRFTDPADWHAAADALAPFGARAEASPRGLDARLPFLDPLE
                     GTHPLGPRLSALLQARHAERHPEMGTGVFLRLFLPADAAPATPDVALNLNVKEREMPF
                     AIDATGAWTIESPEASFEFGTLAGAPRLCYVRFVPNALHLPGLLAALAADMARRADAA
                     REHLHEVISPG"
     gene            complement(4407..4799)
                     /locus_tag="PLB01_15155"
     CDS             complement(4407..4799)
                     /locus_tag="PLB01_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQQ78692.1"
                     /translation="MDERNDSAGTLAGIARSLGIPNARRHIFLCSDPAKPKCCDRDRG
                     VAAWDYLKMRLKELGLADSGEILRTRANCLRVCAGGPIAVVYPEGTWYRGCDPPVLER
                     IITEHLVGGRPVAEYAIAEHELPGGEGV"
     gene            4824..5720
                     /locus_tag="PLB01_15160"
     CDS             4824..5720
                     /locus_tag="PLB01_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ78693.1"
                     /translation="MKIFVTGATGHIGFRIATALRRRGHHVLGLTRADAGAARLARHE
                     IHAVKGTLQDPGTWRGAEDCSTLVHAAADYTTDTMALDRAAVHSLLSLAGRGAGVKTV
                     VYTSGVWVYGSAGDTLVDETSPLAPLPMSVPRVETEKAVLTTHGVKGLVMRPGCVYGY
                     GGSMTAAWFAGAAAGTLEVVGDGSNRWATVHADDVADAYVRAIESGLAGEIFNVTDRS
                     RAPVRRLVLAAARAAGFGGEIRWIPLAEAQQTMGDFATALAFDQHVDSRKAVARLGWQ
                     PTHGGFEDEAATYYASWKAAQG"
     gene            complement(5734..6351)
                     /locus_tag="PLB01_15165"
     CDS             complement(5734..6351)
                     /locus_tag="PLB01_15165"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HQQ78694.1"
                     /translation="MDVPAQFAAHPWHGVDPGPEFPAVFTAFIEIVPTDTVKYELDKA
                     TGILKADRPQLFSNVCPTLYGLVPRTYCGTRVAQLCEERTGRAGLKGDGDPMDVCVLT
                     EKPIAHGQILVPAVPIGGLRMLDNDEVDDKILAVLRGDAVYGALADVTEMPKSLVDRL
                     RHYFLTYKQKPGEPAQSVEITHVYGHGEALAVLAASRDDYRARFG"
BASE COUNT          918 a         2466 c         2324 g          893 t
ORIGIN      
        1 tcgtgcgcgt ccacgacgcc ggcgagacgc tgcgtttcct cgcgacgctc gggcgcacgc
       61 gccgcccggc gctcacgtaa catcggaccc gaatgcgcag gctgttcggc accgacggca
      121 tccgcgggaa ggcgggcgag cctccgctcg acgcggcgac ggtctcgcgc gtcggcgccg
      181 cgctcgcggc gtcgctcggg cacgcgaacc ctgccgtcgt cgtgggctgc gacacgcggg
      241 agtcctcggg cgccatcgtg tgggccctga ccggcggcct cacgaaggcc ggcggcctcg
      301 tgcgcttcgc cggcgtcgtc ccgaccccgg ccgtcgcgtt cctcgcgaag gagctcggtg
      361 ccgacgccgg cgtcgtcgtt tcggcctcgc acaacccgtg gacggacaac ggcgtgaaga
      421 tcttctcggg cgagggccgc aagctccccg acgacgtcga gctcgagatc gagcgtcaca
      481 tcgacgaggc gaagcccgcg cccatggcgc ctgcggtcgt cgagcccgac ctcggccgcg
      541 cgtacgtgga ccacctcgtc gcgacgcttc cccaccgcct cgacggcctg agggtcgtga
      601 tcgacgcggc gaacggcgca gcgttcgagg ccgcgcccgc cgcgttcgcg gccgccggcg
      661 ccaccgtgat cgcccgcaat gcctcgcccg acggccggaa catcaacgag ggctgcggcg
      721 cgctccaccc cggagcgatg cgcgacgccg tccgcgccga gggcgccgac ctcgggatcg
      781 cgctcgacgg cgacgcggac cggatcatcg tcgcggacga cgcgggaacg ctcctcgacg
      841 gcgacgacgt cctcttcctc tggacgctcg agatggagcg cgaaggcaag cggcccgcgt
      901 ccgtcgtcgg caccgtgatg tcgaactggg ggctcgagac ggccctcgcc gggcgcggcg
      961 tcgcgctcgt gcgagcggcc gtcggcgacc gctacgtcgt cgaggagatg gagaagtccg
     1021 gcgcactcct cggcggggaa cagtccgggc acctcatccg cgccgacctc acgacgaccg
     1081 gcgacggcac gctcacgggc ctgcacctcg cggcgctcgt cgcggcgtcg ggcatgaagc
     1141 tctccgcgca gccccgcttc gtccacacgc cgcaggtcct gaagaacgtg aaggtgaagg
     1201 cgcgcgtgcc gttcgaggag atccccggct tcgcgcccct ctggcgcgcc gccgaagcga
     1261 agatgtcggg cgccggccgc attctgctgc gctactcggg caccgaggcg ctcgcccgcg
     1321 tgatggtcga gggccgcgac gcggcgctcg tcgacacggt cgcctcggac ctcgccgccc
     1381 acatcggagc gcgcctcggc gcctgatccc gtggaattcc ctctcgaacc atcggaagag
     1441 cagctgagga cgcttctcga cgacgccgtc cggcgcgtcg tcgcccacgt cgcatcgctt
     1501 ccggcgcagc ccgccgacgg caccaccggg gccggcgatc tcgcgcgcgc cctgatcgag
     1561 cgcgacccgc ccgagcaggg aggcgcggcc gggccgctcc tcgacctcct gttcgaccgg
     1621 gcgattcccg cgagcttcaa caacgcgggc cccggcttcc tcgcgtacat ccccggcggc
     1681 ggccagccct acgccgcggc cgcggacctg atctcggctt ccgtgaaccg ctacacgggc
     1741 gtcttcgcgc cggccccggc gctcgtccag ctcgagacga acgtcatccg ctggttcgcg
     1801 cgcatcgtgg gttacccggg aggcgccggc gggttcctga cgtccggcgg ctccctcgcg
     1861 aacttctccg ccgtcgtcgc ggcgcgccac gaaaaactgc cggagaactt cctgaagggc
     1921 acgctctacg tctccgacca gggccaccac tgcatccgca aggcggcgct cctcgcaggc
     1981 ttcccggatg cgagcgtgcg cgagatcccg tccgatgcgg cgttccggat gcggctcgac
     2041 gccctggccg cgcgggtggc cgcggaccgc gcggcgggtt tcacgccgtt cctcgtcgtc
     2101 gcgagcgccg gcaccacgaa cacgggcgcc gtggacgacc tcgacgccct cgcgaacttc
     2161 gcggcgcacg agggactctg gctccacacc gacgcggcct acggcggctt cttcgcgctg
     2221 acggagcgcg gccgcaaggc gctccacgga ctctcgcgcg ccgactccat cgtcctcgac
     2281 ccgcacaagg gcctcttcat gccctacggc tcgggttgcc tcctcgtgaa ggaccccggc
     2341 gcgctgcgcc gctcgcacag cgtccaggcc cactacatgc cgggcatgca ggaagacccc
     2401 gacagcgtcg acttctgcga gatctcgccc gagctctcgc gcaacttccg cggcctcccc
     2461 gtgtggctcg cgatcaagct gtgcggcctc gcggcgttcc gcgcgtccct ggacgagaag
     2521 ctggacctgg cgcgcgacgc ggtggccgcc gtacgcgcga tccccggcat cgcgatcgcc
     2581 gcagagcctc agctctcgct cttcgccttc cgcctcgccc cgcccggtgc ctccctcgag
     2641 gagtcgaacc gcctgagccg cgagctcatg gcgcgcgtga acgcccgccg gcgggtgctc
     2701 ctgacgggca cgctgctcgg ggaggagttc gccgtccgca tgtgcatcct gagcttcagg
     2761 actcatcggg atcggatcga aatggccctc gacgaccttc gggatgccgc cggcgcgctc
     2821 ctcagggaat aaaccgcccc cgaaccgact ttacgggcgt ggacatgacc cgacgcggcc
     2881 tcttcctcct cctttccctt cccgtcctgg cggccggcgc cctcctggtc gcccgccccc
     2941 agtcgccctc caaaggagct ccgatgaccg ctcccgcctc gcccgccgcc tcgcaagacc
     3001 gcgtgatcaa gaccgacgag gagtggaaga agcttctgac gcccgcgcag tacaacgtca
     3061 cgcgcaagaa gggcacggag cgcgcgttca cgggcccgaa ctgggacaac cacgcgaagg
     3121 gcacgtacgt ctgcgtgtgc tgcgacctgc ctctcttcga ctcggaaacg aagttcgact
     3181 ccggaacggg ctggccgagc ttctggcagc ccatcgcgcc gggcgccgtg aggacggagg
     3241 aggaccgcag cttcttcagc gtgcgcaccg aggtcctgtg ccggcgctgc gacgcgcacc
     3301 tcggccacgt cttcgacgac ggcccgaagc cgaccggcct ccgttactgc atgaacggga
     3361 cggccatgaa gttcatcccg gcgaaatgac ctcgtgcagg tgctcgcgcg ccgcgtccgc
     3421 gcggcgcgcc atgtcggccg cgagggccgc caggagcccc ggcaggtgga gcgcgttcgg
     3481 gacgaaccgg acgtagcaga ggcgcggcgc cccggcgagg gttccgaact cgaagctcgc
     3541 ctcgggcgat tcgatcgtcc acgcgcccgt cgcgtcgatc gcgaacggca tctcgcgctc
     3601 tttcacgttg aggttcaggg cgacgtcggg ggtcgccgga gcggcgtccg ccggcaggaa
     3661 gaggcgcagg aagacgcccg tgcccatttc cggatggcgc tcggcgtgcc gcgcctgcag
     3721 gagcgccgag agccgggggc cgagcggatg cgtcccctcg agcgggtcga ggaacggcag
     3781 ccgtgcgtcc agcccgcgcg gcgaggcttc cgcgcgcgcc ccgaacggcg caagcgcgtc
     3841 cgccgccgcg tgccagtcgg ccgggtccgt gaagcgcgag agatgcttcc cctcgggcgc
     3901 gatgcgcgcc gagaggaggt cgagcatccc gtcggggtgc gggcgcgagc cggacttcgg
     3961 gtgctcgctc gcggcagcgg gcaggccgag ctcgtcttcg agcccttcgc gcccgccctc
     4021 ggcgtagatc gcctgcagcg cggccgacgc cgagaggaag ccgagggcga cgggcgccgt
     4081 ctcccgggtg acgagagccg ccgaccagag ccgcacgacg ccgtcctcgc ggtcgaggac
     4141 gaaggaggcg agaggcggat agcgcagaag gtccgcgagg acgccggcct gccggagcgt
     4201 cacggcgtcc gcgaagagga cgtccgtctc gacgtgaacc cggctggcgg cggcctcgcc
     4261 ctcgccgacc ggctcctcgg cgtggacgcg ctggacgacc cggtgcggcc accacgagaa
     4321 cgaccgctcg tcacggagcg cccaggcgtc gtcgatcagg agacgcgcga agaggtcgtc
     4381 gacgaacgcg gggcccgggt cgacgctcac acgccctcgc cgccggggag ctcatgctcc
     4441 gcgatcgcgt actcggccac gggcctgccg cccacgaggt gctccgtgat gatccgctcg
     4501 aggaccggcg ggtcgcagcc gcgataccac gtcccctcgg ggtagacgac ggcgatcggg
     4561 ccgcccgcgc agacccgcag gcagttcgcc cgcgtccgga ggatctcgcc cgagtccgcg
     4621 aggccgagct ccttgaggcg catcttgagg tagtcccacg ccgcgacgcc gcggtcccgg
     4681 tcgcagcact tcggcttcgc cggatccgag cacaggaaga tgtgccgccg cgcgttcggg
     4741 atcccgagcg accgggcgat ccccgcgagc gtcccggccg aatcgttgcg ctcgtccatg
     4801 aggggagaag gataatcccg ggcatgaaga tcttcgtcac cggcgccacc ggccacatcg
     4861 gattccgcat cgcgacggcc ctccgccgcc gcggccacca cgtcctcggc ctcacgcgcg
     4921 ccgacgcggg cgcggcgcga ctcgcgcgcc acgagatcca cgccgtgaag ggcacgctcc
     4981 aggaccccgg cacctggagg ggagccgagg actgctcgac gctcgtccac gccgccgccg
     5041 actacacgac ggacacgatg gccctcgacc gggcggccgt gcattcgctc ttgtccctcg
     5101 ccggtcgggg ggcgggcgtc aagacggtcg tctacacgtc cggcgtctgg gtctacggat
     5161 ccgcaggcga cacgctcgtg gacgagacgt ccccgctcgc gccgcttccg atgagcgtcc
     5221 cgcgcgtcga gacggaaaag gcggtcctca cgacgcacgg cgtgaagggc ctcgtgatgc
     5281 ggcccggatg cgtctacggc tatggcggca gcatgacggc cgcgtggttc gcgggcgcgg
     5341 cggcgggcac gctcgaggtc gtgggcgacg gctcgaaccg ctgggcgacc gtccacgcgg
     5401 acgacgtcgc cgacgcgtac gtccgcgcga tcgagagcgg cctcgcgggc gagatcttca
     5461 acgtgacgga ccgctcgcgc gcgccggtcc ggcgcctcgt cctcgccgcg gcgcgcgccg
     5521 ccgggttcgg cggcgagatc cgctggatcc cgctcgcgga ggcgcagcag acgatgggtg
     5581 acttcgcgac cgcgctcgcg ttcgaccagc acgtcgactc gcgcaaggcc gtcgcgcgcc
     5641 tcggctggca gcccacgcac ggcggcttcg aggacgaagc cgcgacgtac tacgcctcgt
     5701 ggaaggcggc gcagggctga aggatcgcgt cgctcagccg aatcgcgccc ggtagtcgtc
     5761 gcggctggcc gcgaggaccg cgagggcctc gccatgcccg tagacgtgcg tgatctcgac
     5821 ggactgggcc ggctcgccgg gcttctgctt gtacgtgagg aagtagtggc ggagcctgtc
     5881 cacgagcgac ttcggcatct cggtgacgtc ggcgagcgcg ccgtagacgg cgtcgcccct
     5941 gaggacggcg aggatcttgt cgtcgacctc gtcgttgtcg agcatcctca ggccgccgat
     6001 cgggacggcg ggaacgagga tctgcccgtg cgcgatgggc ttttccgtga ggacgcagac
     6061 gtccatcgga tcgccatcgc ccttgaggcc cgcgcggccg gtcctctcct cgcagagctg
     6121 cgcgacgcgc gtcccgcagt acgtgcgcgg gacgaggccg tagagcgtcg ggcacacgtt
     6181 cgaaaagagc tgcggccggt ccgccttgag gatcccggtc gccttgtcga gctcgtactt
     6241 gacggtgtcc gtcgggacga tctcgatgaa cgccgtgaag accgccggaa actccggacc
     6301 cggatccacg ccgtgccagg ggtgggccgc gaactgcgcg gggacgtcca tgggccaagg
     6361 ttatcctgag cgcgatggaa ccgctctctc ccgccgaccg cgcggcgatc gcggcgcacg
     6421 ggctcaccga ggcggaggtc gcgcggcagc tcgcgctctt acgcgatccg cccgcgcctc
     6481 agcgtctcgc gcgcgccgcg acggcggacc gccgctgccc ggcccgcgcg agatcctgac
     6541 caagcgctgc cagacgtgcc acggcgactt ccaccagcgt gtgatgctcg aagggccgat
     6601 c
//
LOCUS       DAOMFY010000227         6590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3834_length_6590_cov_5.078041, whole genome shotgun sequence.
ACCESSION   DAOMFY010000227 DAOMFY010000000
VERSION     DAOMFY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_6590_cov_5.078041"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLB01_15170"
     CDS             complement(<1..188)
                     /locus_tag="PLB01_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQQ78695.1"
                     /translation="MNATLAALGTPRRREILRLVWEEERSAGAIHGALPDVTFGAVSQ
                     HLRVLRDAGLVACRVEGR"
     gene            complement(269..586)
                     /locus_tag="PLB01_15175"
     CDS             complement(269..586)
                     /locus_tag="PLB01_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HQQ78696.1"
                     /translation="MSTGELDFTGDGGGARLRLRVSAGASRSRVLGVHGGALKLSVKA
                     PPERGKANREVLALVADVFGLPPSDVTLVAGETAPDKVVRLPIPPDEAARRWTVAAGR
                     GRV"
     gene            complement(591..2084)
                     /gene="purF"
                     /locus_tag="PLB01_15180"
     CDS             complement(591..2084)
                     /gene="purF"
                     /locus_tag="PLB01_15180"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQQ78697.1"
                     /translation="MAGAFRDEALDKFRDECGVFGIFGHPDAANLTYLGLYAMQHRGQ
                     ESAGIVTWTGQRMAVEKQMGYVADIFDEARLARLPGHSAIGHVRYSTTGESRLENAQP
                     IVYNTNKGPLAIGHNGNLVNAEEIKRELEQEGSIFTTSSDTEVFLHLMARSRREDVVG
                     ALREALARVRGAYSIVLLAKDRVLAARDPQGIRPLTMGRLRVPGQPDATVVASETCAF
                     DLIDAEPLRDVEPGEIVVFDAGGTASASFAMGQRTAFCVFEHVYFARPDSSTFGKSVA
                     ESRRVFGQRLAREHPVPADVVVPVPDSGMFAALGFAEESGIRYGMGLVRNHYVGRTFI
                     EPKQSIRNFGVKVKLNAVRSVVGGKRVVLVDDSIVRGTTSKKLVRMLKAAGASEVHMR
                     ISSPPTVNPCHYGIDTPRRKELIASAKSLDEIRAFIEADSLGYLSLEGMLAAFGHGDH
                     ETCSACFSGRYPVPLRAPDPQATLFPADEASESAGDEAPVPAPAPRP"
     gene            complement(2139..3287)
                     /locus_tag="PLB01_15185"
     CDS             complement(2139..3287)
                     /locus_tag="PLB01_15185"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQQ78698.1"
                     /translation="MKQNLRTLALAAAAALVTVWAGPALAQPHHLPASGDNQRASVTQ
                     QIGPVDVTISYGSPRVVLRGNDRRGKIWGALVPYGMTELDFNDCKSCPWRAGANEGTV
                     FTTTGEIKVQGKSLPAGSYGLFAITGPEEWTFIFSKNTTSWGAYWYDPKEDVLRVTTK
                     PAKSEYHEWLTYEFTEREPAKATVALKWEDLQVPLALTVDDAPARWVAKMKDALRGYT
                     GFTWQNWQQAADYCLKNKIALPEALDWAQRAAGPNFGGENQLPAQMTLARALAANGKE
                     AEAAKVWDKAVNLPGATPIQIHVVGRTLLTEGKTDQAMKVFQLNAKRYPNQWPVHVGL
                     MRGYAAAGDAKKALAEARLALPQAPDPGNKKGLEDAIAKLEKGDTKIN"
     gene            complement(3402..4079)
                     /locus_tag="PLB01_15190"
     CDS             complement(3402..4079)
                     /locus_tag="PLB01_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQQ78699.1"
                     /translation="MADSLGKIAREIAACRACPRLVAWREAAAGNPPRRFRGQRYWAR
                     PVPGFGDPGARLLVVGLAPAAHGGNRTGRVFTGDASGDFLFAALHRANFANQAVSVSR
                     EDGLELTGAYVAAAARCAPPDNKPTPAEFARCLPFLAREIAQLPELRVVLALGAHGWR
                     AAIEALAANGAPRPKPLPRFAHGAEAQVGGVRLIASYHVSQQNTFTGRLTPKMLDVVL
                     RRTREIS"
     gene            complement(4093..4407)
                     /locus_tag="PLB01_15195"
     CDS             complement(4093..4407)
                     /locus_tag="PLB01_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78700.1"
                     /translation="MNVNFRRALGGTLAVALVSAGLLSGCSKKGSLEKAGAAADKAIE
                     KAKDAAAETAKSYGAAAEKAADKAVVATKEAAEKAAAATKEAAASAAKATSTAVAPVA
                     KH"
     gene            complement(4404..5201)
                     /locus_tag="PLB01_15200"
     CDS             complement(4404..5201)
                     /locus_tag="PLB01_15200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ78701.1"
                     /translation="MRAPLSVVILTKNEEDRLPAALASVAFADEVVVADTGSTDATEE
                     RARLAGARFVRIGWDGFVGSRNKALALARHDWVLVLDADERVPEALRREILAALDDAD
                     PAVAGWRMPRLSHFLGRPIRHGSWYPDYKLRLGRRSRGLRAEGGRVHEALAVDGSVER
                     LATPLIHHPYRDLSDALRKASRYAQLGAEDRYDRGARGSAAGLFLRPAFEFFRFLVFK
                     RGFLDGSVGWSVAFLHASSYFLRAAFLLEIQRRPETGKHPGRPKEES"
     gene            complement(5279..5785)
                     /gene="rnc"
                     /locus_tag="PLB01_15205"
                     /pseudo
     CDS             complement(5279..5785)
                     /gene="rnc"
                     /locus_tag="PLB01_15205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
     gene            complement(5817..6194)
                     /locus_tag="PLB01_15210"
     CDS             complement(5817..6194)
                     /locus_tag="PLB01_15210"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HQQ78702.1"
                     /translation="MRNRRRQQGIGFIGLSFVVIVVGALVLLGFRLLPAYLQFLTVKS
                     ALKEVTANPELRNAGAPEIRSAFDKRAMVNDIKVISSQDLDIEKDGEGGFTVSANYSQ
                     QIPLFQNVSACIDFSTTSSTGGK"
     gene            complement(6204..>6590)
                     /gene="lepB"
                     /locus_tag="PLB01_15215"
     CDS             complement(6204..>6590)
                     /gene="lepB"
                     /locus_tag="PLB01_15215"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQQ78703.1"
                     /translation="RLWVNGERVPTKPDGVYSYVEGSLALVTADRYVEDLGGHDHSII
                     VNPQVPPIQVAGVRQFAYRDACVYNDDGFRCKVPAGHFFMMGDNRDASSDSRYWGFVP
                     ERNIVGKAFVIWWNFDDLSRIGRSIN"
BASE COUNT          968 a         2306 c         2283 g         1033 t
ORIGIN      
        1 atgcggccct cgacccggca cgcgaccagg cccgcgtccc tgaggacgcg gaggtgctgc
       61 gacaccgcgc cgaaggtgac gtccgggagc gccccgtgga tcgcccccgc gctcctctct
      121 tcctcccaga cgagacgcag gatctcgcgg cgacgcggcg tcccgagagc ggcgagggtg
      181 gcgttcatgg cgtccacgca tttaatttag taattactaa ataaaagtca agggaaaacc
      241 gccggtttcc cggcggcgag tcgaaaccct aaacccgccc gcggccggcc gcgacggtcc
      301 accgccgggc cgcctcgtcc ggtggaatcg gcagccggac gaccttgtcg ggcgccgtct
      361 cccccgcgac gagcgtcacg tccgacggcg ggaggccgaa cacgtccgcc acgagggcga
      421 gcacctcgcg gttcgccttg ccccgctccg gcggtgcctt gaccgagagc ttcagggcgc
      481 ctccgtgcac gccgaggacg cgcgagcgcg aggcgcccgc cgagacgcga aggcgcagcc
      541 tcgccccgcc gccgtcgccc gtgaagtcga gctcgcccgt gctcacgcgc tcagggccgc
      601 ggggcgggcg ccggaaccgg ggcctcgtct cccgcgctct cggaggcctc gtccgccggg
      661 aagagcgtgg cctgcgggtc gggcgcgcgg agcggcaccg gataccggcc cgagaagcag
      721 gccgagcacg tctcgtggtc gccgtgtccg aaggccgcga gcatgccctc gaggctcagg
      781 taaccgaggg agtcggcctc gatgaacgcc cggatctcgt cgaggctctt cgccgacgcg
      841 atgagctcct tgcggcgggg cgtgtcgatc ccgtaatggc aggggttcac ggtgggcggc
      901 gacgagatcc gcatgtggac ctcgctcgcg cccgcggcct tgagcatccg gacgagcttc
      961 ttcgacgtcg tcccgcgcac gatcgaatcg tccacgagga ccacgcgctt gccgccgacg
     1021 accgagcgga cggcgttcag cttcaccttc acgccgaagt tgcggatgga ctgcttcggc
     1081 tcgatgaacg tgcggcccac gtagtggttc cggacgaggc ccattccgta ccggatcccg
     1141 ctctcctcgg cgaatccgag cgccgcgaac atcccggagt ccggcaccgg cacgacgacg
     1201 tcggccggca ccgggtgctc gcgggcgagc cgctggccga agacgcgccg cgactccgcg
     1261 accgatttgc cgaaggtgga cgagtcgggc cgcgcgaagt agacgtgctc gaagacgcag
     1321 aaggcggtcc gctggcccat cgcgaaggaa gccgaggccg tgcccccggc gtcgaagacg
     1381 acgatctcgc cgggttcgac gtcgcgcagc ggctcggcgt cgatgaggtc gaacgcgcac
     1441 gtctccgagg cgacgacggt cgcatccggc tgccccggca cccggagccg gcccatcgtg
     1501 agcgggcgga tcccctgcgg gtcgcgggcc gcgaggacgc ggtccttcgc gaggaggacg
     1561 atcgagtagg ccccgcggac gcgcgcgagc gcctcgcgca gcgcgccgac gacgtcctcg
     1621 cgccgcgagc gcgccatgag atggaggaag acctccgtgt ccgacgacgt cgtgaagatc
     1681 gagccttcct gctcgagctc gcgcttgatc tcttcggcgt tcacgaggtt cccgttgtgg
     1741 ccgatcgcga gcgggccctt gttcgtgttg tagacgatcg gctgggcgtt ctcgaggcgc
     1801 gactcgcccg tcgtcgagta gcggacgtgg ccgatcgccg agtgcccggg caggcgcgcg
     1861 agacgcgcct cgtcgaaaat gtccgccacg tagcccatct gcttctcgac ggccatgcgc
     1921 tggcccgtcc acgtcacgat tcccgcggac tcctgcccac ggtgctgcat cgcgtagagg
     1981 ccgaggtacg tcaggttcgc ggcgtccggg tggccgaaga tgccgaaaac gccgcactcg
     2041 tcgcggaact tgtccagcgc ttcgtcccgg aaggcccccg ccacgcaggg agtctaatgg
     2101 aaacggcgcc ggcggtgcct gccgccggcg ccccgagttc agttgatctt cgtgtcgccc
     2161 ttctcgagct tcgcgatcgc gtcctcgagt cccttcttgt tgcccgggtc cggcgcctgc
     2221 ggaagcgcga ggcgggcctc cgcgagagcc ttctttgcgt cgcccgccgc cgcgtagccg
     2281 cgcatgaggc cgacgtggac gggccactgg ttcgggtagc gcttcgcgtt cagctggaag
     2341 accttcatcg cctggtcggt cttcccctcc gtgaggagcg tgcgtccgac cacgtggatc
     2401 tggatcggcg tggcgcccgg caggttcacg gccttgtccc agaccttcgc ggcctccgcc
     2461 tccttgccgt tcgcggcgag ggctctggcg agcgtcatct gcgcggggag ctggttctcg
     2521 ccgccgaagt tcggcccggc cgcccgctgc gcccagtcga gcgcctcggg gagcgcgatc
     2581 ttgttcttga ggcagtaatc cgcggcctgc tgccagttct gccacgtgaa gcccgtgtat
     2641 ccgcggagcg cgtccttcat cttcgcgacc cagcgcgccg gcgcgtcgtc gaccgtcaga
     2701 gcgagcggga cctgcaggtc ctcccacttc agggcgacgg tcgccttcgc gggttcgcgc
     2761 tccgtgaact cgtacgtgag ccactcgtgg tactcgctct tcgcgggctt cgtcgtgacc
     2821 ctgaggacgt cttccttcgg gtcgtaccag tacgcgcccc aagacgtcgt gttcttcgag
     2881 aagatgaacg tccactcctc cggtcccgtg atcgcgaaga ggccgtagga gccggccggg
     2941 agcgacttgc cctgcacctt gatctccccc gtggtcgtga agaccgtgcc ctcgttggcg
     3001 ccggcgcgcc acgggcacga cttgcagtcg ttgaagtcca gctcggtcat cccgtagggg
     3061 acgagcgccc cccagatctt gccgcggcgg tcgttccccc tgaggacgac gcgcggactt
     3121 ccgtacgaga tcgtgacgtc gacgggcccg atctgctggg tgaccgaggc ccgctggttg
     3181 tccccgctcg cggggaggtg atgcggctgg gcgagggcgg gacccgccca aacggtgacg
     3241 agcgcggcgg ctgccgcgag cgcgagggta cgcaggttct gtttcatggt ggctgatacg
     3301 caccgggaat ggacgtgttt cttagaaggt gaatgggacg acgccatggc ccgcctacgg
     3361 gcctcgcgaa ccggctccga accgcccccg cacccaactc tctaggaaat ctctctcgtt
     3421 ctcctcagga ccacatccag catcttcggc gtcaggcgcc ccgtaaaggt gttctgctgg
     3481 ctgacgtggt acgaggcgat taggcgcacg ccgccgactt gcgcttccgc gccgtgggcg
     3541 aatctcggga ggggcttcgg gcgcggggcg ccgttcgcgg ccagcgcctc gatcgcggcc
     3601 ctccagccgt gcgcaccgag tgcgaggacg accctcagct cggggagctg ggcgatctcg
     3661 cgggcgagga acgggaggca gcgcgcgaac tctgccggtg tcggcttgtt gtccggcggg
     3721 gcgcagcgcg ccgcggccgc aacgtacgct cccgtcagct cgaggccgtc ctcccgcgaa
     3781 acggaaaccg cctggttcgc gaagttcgcc ctgtggagcg cggcgaacag gaaatcgccg
     3841 gaggcgtcgc ccgtgaagac gcggcccgtc cggttgcctc cgtgcgcggc gggcgcgagt
     3901 ccgaccacga ggagacgcgc cccggggtcg ccgaagcccg gcaccggacg ggcccagtag
     3961 cgctgtcccc ggaagcgccg gggcgggttc ccggccgcgg cctcgcgcca cgcgacgagg
     4021 cgagggcagg cgcggcaggc ggcgatctcc cgtgcgatct tcccgaggga gtccgccacg
     4081 cggcgggcgg ccctagtgct tggcgacggg cgcgacggcg gtgctcgtgg ccttcgccgc
     4141 gctcgcggcc gcttccttcg ttgcggcggc ggccttttcg gccgcttcct tcgtggcgac
     4201 gacggccttg tcggcggcct tctcggcagc ggcgccgtac gacttggcgg tctcggccgc
     4261 cgcgtccttg gccttctcga tcgccttgtc ggcggccgcg ccggcctttt ccagggaccc
     4321 cttcttcgag cagcccgaga gaaggcctgc ggagacgagc gcgacggcga gcgtcccgcc
     4381 cagcgcgcgg cggaagttga cgttcatgat tcctccttcg gtcggccggg atgctttccc
     4441 gtttcggggc gtcgctggat ctcgagcaga aacgcggcgc gcagaaagta cgacgacgca
     4501 tggaggaagg caaccgacca gccgacggag ccgtccagga atccccgctt gaagacgaga
     4561 aaccggaaga attcgaacgc cggccggaga aagaggcccg ccgcgctgcc gcgcgctccc
     4621 cggtcgtaac ggtcctcggc gccgagctgc gcgtagcgag acgccttccg gagcgcgtcc
     4681 gaaaggtcgc ggtacggatg gtggatgagc ggcgtggcga gccgctcgac gcttccgtcg
     4741 accgcgagcg cctcgtggac gcgtccgccc tcggcgcgaa gcccgcggga ccgccgcccg
     4801 agacggagct tgtaatccgg gtaccagctg ccgtgccgga tcggacgccc gagaaaatgg
     4861 gacagacgcg gcatgcgcca acccgccacg gcggggtcgg cgtcgtcgag ggccgcgagg
     4921 atctcccgtc gaagcgcttc cgggacgcgc tcgtcggcat cgaggacgag gacccagtcg
     4981 tgacgcgcga gcgcgagcgc cttgtttcgc gaccccacga agccgtccca gccgatgcgg
     5041 acgaaacgcg cgcccgcgag acgcgcccgc tcctcggtgg cgtccgtcga acccgtgtcg
     5101 gcgacgacga cctcgtccgc gaaggccacc gaggcgaggg ccgccggcag gcgatcctcc
     5161 tcgttcttcg tcaggatcac gaccgagagc ggcgcgcgca cggcgcggac attaacaggc
     5221 gctatcctcg tccgccgtga ggctgctggc gctggatctc gggcccggaa tgcgcggggg
     5281 gcagcgccag ccggcgcccc tggagatatt cctgcagctg cgtcttcggg tccttcccct
     5341 gcgtgcgtgg atcgatgccg gcaagcagcg gtgagaagca gcctgccacg acgtcgcgcg
     5401 ccgcctcgaa ctggccatcg acgaacaccg cgccgctcac cgcctcgagc gcatcggcga
     5461 gaatcgaggg tcggcgcgcc ccgccgctcc tgagttcccc ctcgcccagc tgcagcgcat
     5521 cgccgagctt gagcgtctgc gccacgttga agaggctctc ctgattcacc agacttgcgc
     5581 gggtgcgcga gagttccccc tcgctcaacc gtggaaagcg ctcgtagagg agcgccgcga
     5641 tgacgcagtt gagaacgctg tcgcccagga actccaggcg ctcgttgtgg ctcgcgctgt
     5701 ggctgcgatg ggtcagcgcc tgacgcagca gcgcgggctc acgaaatcgg taaccgatgc
     5761 cgcgctcggt ggctgagaga tccatctgac gctgatggcg tcgcgctcac gacccgctac
     5821 ttcccgcccg tggagctcgt ggtcgagaaa tcgatgcacg cgctcacgtt ctgaaacagc
     5881 ggaatctgct gggaatagtt ggccgacacg gtgaaaccgc cttcaccgtc cttctcgatg
     5941 tcgagatcct ggctgctgat gaccttgatg tcgttcacca tcgcgcgctt gtcgaacgcg
     6001 ctgcggatct cgggcgcacc ggcattcctg agctcgggat tggcggtgac ttccttcagg
     6061 gcgctcttca ccgtcaggaa ctgcagatac gcgggcaaga ggcgaaagcc cagcagcacg
     6121 agggccccca ccacgatcac cacgaacgag agcccgatga acccgatccc ctgctgccga
     6181 cgtcgattgc gcatgctgtc ccctcaattg atgctgcggc cgatgcgcga caggtcgtcg
     6241 aagttccacc agatgacgaa tgccttgccg acgatgttgc gctccggcac gaaaccccag
     6301 taccggctgt cgctgctcgc atcgcggttg tcccccatca tgaagaagtg acccgcgggc
     6361 accttgcagc ggaagccgtc gtcattgtag acacaagcgt cgcggtaggc gaactgccgc
     6421 acgcccgcca cctggatcgg cggcacctgc gggttgacga tgatgctgtg gtcgtggccg
     6481 ccgaggtcct cgacgtagcg gtccgcagtc accagggcga ggctgccttc gacgtaggaa
     6541 tagacgccgt ccggcttggt cggcacccgc tcgccgttca cccacaggcg
//
LOCUS       DAOMFY010000228         6522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3895_length_6522_cov_10.295346, whole genome shotgun sequence.
ACCESSION   DAOMFY010000228 DAOMFY010000000
VERSION     DAOMFY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3895_length_6522_cov_10.295346"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLB01_15220"
     CDS             complement(<1..656)
                     /locus_tag="PLB01_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HQQ78704.1"
                     /translation="MSAARAPRPRLVLFDIDGTLLSGGRAARTVFAGALTEVFGTCGD
                     VDTFAFEGKLDPMIVRELLLAAGVPEETVLRRRADALALYLDRLEAALAAERPVLKPG
                     VAAVLDGVARSGGTVQALLTGNVERGARIKLSAVGLWDRFAFGAFGDEAPRREDLGPV
                     ALEKARRLGHAFRGPDCVVVGDAPQDVACALALGARIVARIAGDRQPGARFQAGEVVL
                     "
     gene            complement(653..1528)
                     /locus_tag="PLB01_15225"
     CDS             complement(653..1528)
                     /locus_tag="PLB01_15225"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQQ78705.1"
                     /translation="MGRRIACVLLALLAAAAPARAARRDERVPTRNMPGPVPVAVATP
                     PSYDATPLRRYPVLYFLHDGQGDEAVLFRQGVIDALDAAMRAGRLPEMIIVCPRGKGT
                     WWVDAQGGARRMAAFLTDDLVPFVDRTFRTRPSRDGRVAAGISMGGYGALRWALARPD
                     LFTAAGGLSPAIQQLCVPSVAALPFFLRPSLEAVFGADPVANVLRRNDLYQMLLDDPS
                     LAARAPFLLVRCGTEDKYRLAEIAGFFGRFASAVGAAHEVRLETGVHDWPYWRRVFPD
                     FAASLAARLPSGETP"
     gene            1562..2302
                     /locus_tag="PLB01_15230"
     CDS             1562..2302
                     /locus_tag="PLB01_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ78706.1"
                     /translation="MPRFLEGQTAIVTGGGRGIGRAIALALAREGATVAVASRSADEV
                     ERTAAEIRARAGRALAIPTDVTSRAAVEALVAQTLEETQRLDVVVNNAGVFVWRAFAK
                     LEEDDWDRILDTNLKASYLLARAALPALSASGHGRIVNVASIHGSVGEASVVAHCAAK
                     FGLIGLTKALAAELRTVGVTVNAVSPGATDNKSLDLDPPAHAEPLKEKLHARDVAEAV
                     LFLVSPAATTISGAVLDVWGGTSLAIRT"
     gene            complement(2324..2956)
                     /locus_tag="PLB01_15235"
     CDS             complement(2324..2956)
                     /locus_tag="PLB01_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQQ78707.1"
                     /translation="MPTRRASLATLAALASLALSSSARAGTKVKESWADPQFGKMKFK
                     NVLAIVATKDASLRRTAEDEICRNVVKTACTRGYTILSEADAVDKEKAVAAIKAGGFD
                     GVVLLRALGGDGGMTREAGAARPTYYWTFYTYYGYWGTSWGVPYVAANTSYTKTQTYV
                     TLETNIYDMATDKLVWGGSTETKNPDSARELVRDVTKAVRKDLKAKGLVD"
     gene            3079..4884
                     /locus_tag="PLB01_15240"
     CDS             3079..4884
                     /locus_tag="PLB01_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQQ78708.1"
                     /translation="MAVGRSSTAETSRRDFLRMAGTGATALTFGVGAASGAEPPGPGV
                     SGAPAAEASRSGRYNILFLLTDQERFFRPGELPAGFRLPGHERLRSRGVSFVNHRINS
                     CVCTPSRSVIYTGRHIQQTRMFDNTNFPWTKSLSPEIPTVGHRLRRAGYYTAYKGKWH
                     LTKEFETVNTLGTPTKIFTKEMEEYGFSDYFGVGDIIAHTRGGYLHDGVIASMGVSWL
                     RGKGRDLAAEGKPWFLAVNLVNPHDVMFYDTDAPGTPVQSKRGITHVARDPADPLYAT
                     QWDFRLPASHAQPLDAPGRPRAHLDFLRSHDALVGAIPDEEARWRRRHNYYLNCLRDV
                     DRNIVMLLDELDASGLADRTIVVLTADHGDMDGAHKLHAKGAVVYREQNQVPLLVAHP
                     AHPGGRECRAITSHLDLAPTLVALAGPAADQKPSLVDGLPGKDLSGLLAAPETAAVDA
                     VHAGTLFNYNMFAYLDGEYLNKAVDYVQKGGNPKELKAAGLVPDMMKRGAVRSVHDGR
                     YVFARYFSPKQHNRPTTLEDIYRNNDVELFDTTADPLEMTNLAAGDRKHRDLVLAMNE
                     KLNLLIDREVGEDRGQMLPGGVEAGWEVTAETMAP"
     gene            complement(4906..5733)
                     /locus_tag="PLB01_15245"
     CDS             complement(4906..5733)
                     /locus_tag="PLB01_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQQ78709.1"
                     /translation="MKKAILLLSMLGLLPLRAAVVQKDAVNCADHPLFTRMPESWIHS
                     CSQKTFDAHAFPVAKDKTERVEGRKWTINYYPQAAAKEKPSELQILRNFENAVRKIGG
                     TVVFADKAKETFRIARDGKEFWVDLRAEFTGKYFLTIVEKEAMAQDIVGNADAFSNDI
                     RATGHAAVYGIHFDTAKTEIKPESARAIAEIAKLLNGDAALKLFVVGHTDLVGGVDGN
                     LKLSQGRADAVVKALVEGHGIAAARLRAFGVGPFAPVASNDSEDGRAKNRRVELVKQ"
     gene            complement(5815..6354)
                     /locus_tag="PLB01_15250"
     CDS             complement(5815..6354)
                     /locus_tag="PLB01_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78710.1"
                     /translation="MRRALPILAALLLASPPAGAQDQAGRAPSDYGLRSSRFTIGGGI
                     GFGFGSVNWVSVSGELGYLPADRVWVGTNGMFRYTNDTRYEPSFNATDYGVGVFGRYF
                     VFDRFFADTEWAWTSYESRTYGASRSNITSFLVGGGYGAPLGGSSSAMLEVLYDVTGN
                     ANGVYGTPWVVRAGFATGF"
     gene            6445..6522
                     /locus_tag="PLB01_15255"
     tRNA            6445..6522
                     /locus_tag="PLB01_15255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6480..6482,aa:Arg,seq:tct)
BASE COUNT         1004 a         2274 c         2313 g          931 t
ORIGIN      
        1 tcgagaacaa cctcacccgc ctggaagcgc gcgcccggct ggcgatcacc ggcaatccgg
       61 gcgacgatgc gagcgccgag cgcgagggcg caggcgacgt cctgcggcgc gtcgccgacc
      121 acgacgcagt ctggtccgcg gaacgcgtgg cccaggcggc gggccttctc gagagccacc
      181 gggccgaggt cctcgcggcg cggcgcttcg tcgccgaacg cgccgaacgc gaaccggtcc
      241 cagaggccga cggcggagag cttgatgcgc gcaccgcgct cgacgttccc cgtgaggaga
      301 gcctggaccg tgccgccgga gcgggcgacg ccgtcgagaa ccgccgcgac gcccggcttg
      361 aggaccggtc gctcggcggc gagcgcggcc tccaggcggt cgaggtagag cgccagggcg
      421 tcggcacggc gtcggaggac ggtctcctcg gggacgcccg cggcgaggag gagctctctc
      481 acgatcatcg gatcgagctt gccttcgaac gcgaacgtgt cgacgtcgcc gcacgtcccg
      541 aagacttccg tgagcgcgcc ggcgaagacc gtgcgcgcgg cccggccgcc cgagaggagc
      601 gtcccgtcga tgtcgaacag gacgagccgc gggcgcggcg cgcgggcggc gctcacgggg
      661 tctccccgga cgggaggcgc gcggcgaggg aggcggcgaa gtcgggaaac acgcgtctcc
      721 agtacggcca gtcgtgcacg ccggtttcga gccggacctc gtgggccgcg ccgacggccg
      781 aggcgaagcg gccgaagaat ccggcgatct ccgcgaggcg gtacttgtcc tccgtgccgc
      841 agcgcacgag cagaaacggc gcccgtgccg ccagggaggg gtcgtcgagg agcatctggt
      901 aaagatcgtt ccggcgcagg acgttcgcga ccggatcggc gccgaagacg gcttcgagag
      961 acggcctgag gaagaacggg agcgcggcga cggagggaac gcacagctgc tggatcgcgg
     1021 gcgaaaggcc gcccgccgcg gtgaagaggt ccggccgggc gagggcccag cgcagggcgc
     1081 cgtagccgcc catcgagatg cctgcggcga cgcggccgtc acgcgagggg cgggtgcgga
     1141 aagtccggtc cacgaacggg acgaggtcgt ccgtgaggaa cgcggccatc cggcgcgcgc
     1201 cgccctgcgc gtccacccac cacgtgccct ttccccgcgg acagacgatg atcatctcgg
     1261 ggagacgccc ggcgcgcatc gccgcgtcga gcgcgtcgat cacgccctgc cggaagagca
     1321 ccgcctcgtc gccctgcccg tcgtgcagga agtagaggac cgggtaccgc cggagcggcg
     1381 ttgcgtcgta agaaggcggc gtcgcgacgg cgacgggaac cggccccggc atgttgcgcg
     1441 tgggcacgcg ctcgtcgcgc cgcgccgccc ttgccggcgc ggcggcggcg agcagcgcga
     1501 ggagcacgca cgcgatacga cgtcccatga cgggattcta ggggcctgct aacctttctc
     1561 catgcctcgc ttcctcgaag gccagacggc gatcgtgacg ggcggcgggc gcgggatcgg
     1621 gcgcgcgatc gcgctcgcgc tcgcgcgcga gggggcgacg gtcgccgtcg cgtcgcgttc
     1681 cgccgacgaa gtcgaacgga cggcggccga gatccgtgcg cgcgccgggc gggcgctggc
     1741 gatccccacg gacgtgacgt cccgggcggc cgtcgaggcc ctcgtggcgc agacgctgga
     1801 ggagacgcag cgcctcgacg tcgtcgtgaa caacgcgggc gtcttcgtgt ggcgggcgtt
     1861 cgcgaagctc gaagaggacg actgggaccg gatcctcgac acgaacctca aggcgagtta
     1921 cctgctcgcg cgggccgcgc tcccggcgct ctcggcgtcg gggcacggcc gcatcgtgaa
     1981 cgtcgcgtcg atccacggct ccgtcggcga ggcgagtgtc gtcgcgcact gcgcggcgaa
     2041 attcgggctc atcgggctca cgaaggccct cgcggccgag ctacggaccg tcggcgtcac
     2101 ggtcaacgcc gtcagcccgg gcgcgacgga caacaagagc ctcgacctcg acccgcccgc
     2161 ccacgccgag cccctgaagg aaaagctcca cgcgcgcgac gtggccgagg cggtcctctt
     2221 cctcgtctcg cccgccgcga ccaccatctc gggtgccgtg ctcgacgtgt ggggcgggac
     2281 gtccctcgcg atccggacgt aggaatcggc cggaggggcg tcgctagtcg acgaggccct
     2341 tggccttcag gtccttgcgg acggcctttg tgacgtcccg gacgagctcc cgggccgagt
     2401 cggggttctt ggtctcggtc gagccccccc agacgagctt gtcggtcgcc atgtcgtaga
     2461 tgttcgtttc gagcgtgacg taggtctgcg tcttcgtgta gctcgtgttc gcggcgacgt
     2521 acgggacgcc ccagctcgtg ccccagtagc cgtagtacgt gtagaacgtc cagtagtacg
     2581 tcgggcgggc ggcgccggcc tcgcgggtca tgccgccgtc cccgccgagc gcgcggagga
     2641 ggacgacgcc gtcgaagccg cccgccttga tcgccgcgac cgccttctcc ttgtcgaccg
     2701 cgtcggcctc ggagaggatc gtgtagccgc gcgtgcacgc ggtcttcacg acgttcctgc
     2761 agatctcgtc ctcggccgtg cggcggaggg acgcgtcctt cgtcgcgacg atcgcgagga
     2821 cgttcttgaa cttcatcttg ccgaactgcg ggtcggccca gctctccttg accttcgtgc
     2881 cggcgcgggc ggacgaggag agcgcgagcg aagcgagggc ggcgagggtc gcgagggagg
     2941 cgcgtcttgt cggcatggcg cgagagctta gccgatgggc ggcgccacgg tgattccgcg
     3001 tctccggccg cacccggtcc ggggggcagg gcattgctaa gatccctcga aatttttcgg
     3061 aacgagggag ggccagcgat ggcggttggt cgttcgtcta ccgccgagac ttcgcggcgt
     3121 gactttcttc gcatggccgg caccggggcg accgcgctca ccttcggggt gggggccgcg
     3181 agcggcgccg aacccccggg ccccggcgtc tccggagcgc cggctgcgga ggcctcccgc
     3241 tccggccggt acaacattct cttcctgctg accgaccagg aacgcttctt ccgcccggga
     3301 gagctgccgg cggggttccg cctccccggc cacgagcgcc tgcgctcgcg cggcgtgtcg
     3361 ttcgtgaatc accgcatcaa ctcctgcgtc tgcacgccgt cgcgctcggt gatctacacc
     3421 ggccggcaca tccagcagac ccggatgttc gacaacacga acttcccgtg gacgaagagc
     3481 ctgtcgccgg agatcccgac ggtgggccac cggctccggc gggcggggta ctacacggcc
     3541 tacaagggga agtggcacct gaccaaggag ttcgaaaccg tcaacacgct cggcacgccg
     3601 acgaagatct tcacgaagga aatggaggag tacggcttct ccgactactt cggcgtgggg
     3661 gacatcatcg cccacacgcg cggcggttac ctgcacgacg gggtcatcgc ctcgatgggg
     3721 gtgagctggc tgcgcgggaa gggacgcgac ctggccgcgg agggaaagcc ctggttcctc
     3781 gcggtcaacc tcgtgaaccc ccacgacgtg atgttctacg acacggacgc gccggggacg
     3841 ccggtgcagt cgaagcgcgg catcacgcac gtggcgcgcg atcccgccga cccgctctac
     3901 gcgacgcagt gggacttccg gctcccggcg agccacgcgc agcccctcga cgcgccgggc
     3961 cggccgcgcg cgcacctcga tttcctccgg tcgcacgacg cgctcgtcgg cgccatcccc
     4021 gacgaggagg cccgctggcg ccggcggcac aactactacc tcaactgcct gcgcgacgtg
     4081 gatcgcaaca tcgtgatgct gctggacgag ctagacgcgt cgggcctcgc ggaccggacg
     4141 atcgtcgtcc tcacggcgga ccacggcgac atggacgggg cgcacaagct ccatgcgaaa
     4201 ggggcggtgg tgtaccggga gcagaaccag gtcccgctcc tcgtcgcgca cccggcccac
     4261 ccgggcggcc gggagtgccg cgccatcacc tctcacctcg acctcgcgcc cacgctcgtc
     4321 gccctggccg gacccgccgc ggatcagaag ccgagcctcg tcgacggact ccccggcaag
     4381 gatctctccg gcctcctcgc ggcgcccgaa accgcggcgg tcgacgccgt ccacgcgggg
     4441 acgctcttca actacaacat gttcgcctat ctggacggcg agtacctcaa caaggccgtc
     4501 gactacgtgc aaaagggagg gaatcccaag gagctgaagg ccgccgggct cgtcccggac
     4561 atgatgaagc gaggggcggt gcggtccgtc cacgacgggc gctacgtctt cgcgcgctac
     4621 ttctcgccga agcagcacaa ccgcccgacg accctcgagg acatctaccg gaacaacgac
     4681 gtcgagctct tcgacacgac cgcggacccc ctcgagatga cgaacctcgc cgcgggcgac
     4741 cggaagcacc gggacctcgt cctcgccatg aacgaaaaac tcaacctcct gatcgaccgg
     4801 gaggtgggcg aagaccgggg gcagatgctc cccggcggcg tggaggcggg ctgggaggtc
     4861 accgccgaga cgatggcgcc ctgaacgccc cgggtggaag ccctcctact gcttgacgag
     4921 ctcgacgcgg cggttcttcg cccgtccgtc ctcggagtcg ttcgaggcga ccggcgcgaa
     4981 cgggcccacc ccgaaggccc tgagccgggc cgcggcgatt ccgtgcccct cgacgagcgc
     5041 cttcacgacg gcatccgccc gcccctgcga gagcttgagg ttgccgtcga ctccgcccac
     5101 gaggtcggtg tgtccgacga cgaagagctt gagggcggcg tccccgttca ggagcttcgc
     5161 aatctccgcg atcgcccgcg ccgactccgg cttgatctcg gtcttcgccg tgtcgaagtg
     5221 gatgccgtag accgcggcgt gcccggtcgc gcggatgtcg ttcgagaagg cgtcggcgtt
     5281 gccgacgatg tcctgcgcca tcgcctcctt ctcgacgatc gtcaggaagt acttgcccgt
     5341 gaactcggcc cggaggtcga cccagaactc cttcccgtcg cgcgcgatcc ggaaggtctc
     5401 cttggccttg tcggcgaaga cgaccgtgcc cccgatcttc cggacggcgt tctcgaagtt
     5461 gcggaggatc tggagctcgc tgggcttctc cttcgccgcc gcctgcgggt agtagttgat
     5521 cgtccacttc cggccctcga cccgctccgt cttgtccttg gcgacgggga aggcgtgggc
     5581 gtcgaacgtc ttctgagagc agctgtggat ccagctctcc ggcatccgcg tgaagagcgg
     5641 gtggtccgcg cagttcacgg cgtccttctg caccacggcg gcgcggaggg gcagcaggcc
     5701 aagcatcgac aggaggagga tcgccttctt catgtacgac tccttgacct caagatggtt
     5761 cgggagccgc gttcgcgcaa gcgggtccgg cccgacgtgg caggacgcgg ccgctcagaa
     5821 tccggtcgcg aagccggcgc ggacgaccca gggggtaccg tagacaccgt tcgcgtttcc
     5881 cgtgacgtcg tacaggacct ccagcatcgc gctgctgctg ccgccgagcg gcgcgccgta
     5941 cccgccgccg acgaggaagc tcgtgatgtt gctccgggag gcgccgtagg tcctggactc
     6001 gtaactcgtc caggcccact cggtgtccgc gaagaagcgg tcgaagacga agtaccgtcc
     6061 gaagacgcca acgccgtagt cggtcgcgtt gaagctcggc tcgtaacgcg tgtcgttcgt
     6121 gtaacggaac atgccattcg ttccgaccca gacgcgatcc gccgggaggt agccgagctc
     6181 gccggagacc gacacccagt tcacggaccc gaagccgaac ccgatccccc ctccgatcgt
     6241 gaagcggctg gaacgcagcc cgtagtcgga gggagcacgg cccgcctggt cctgcgcccc
     6301 cgcgggcggc gaggcgagga gcagggcggc gagaatcggc aacgctcgcc tcacgatgtg
     6361 attttcacac gcggccgccc cgcaggggcg cgcacggccc gacggcggtg ctacagtgat
     6421 ttcgcgttcg gctgacgtcc gtgcctgccc gtagctcagc tggataagag catcggcctt
     6481 ctaagccgag ggtcgggagt tcgaatctcc ccgggcaggc ca
//
LOCUS       DAOMFY010000229         6499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3916_length_6499_cov_7.090161, whole genome shotgun sequence.
ACCESSION   DAOMFY010000229 DAOMFY010000000
VERSION     DAOMFY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3916_length_6499_cov_7.090161"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="PLB01_15260"
     CDS             <1..1788
                     /locus_tag="PLB01_15260"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HQQ78711.1"
                     /translation="ERIAFLLAVGLFSGFLFFGLEVVWTHLIGAVLGNSIYAFAAMLA
                     LVLAGLGIGGALSTRLLREERPISGGALGAMLLAGSLLLAWQFTLWPRIPNEFIRWGG
                     GLTTFAQGEMLRWAQAARLLLPPAIVFGMVYPALFRMEAFPLRRRARHAAALGAMNSV
                     GCVLGALLTGFFLIPALGSEATLILFGTLALAAGVLVALAHDRGWRRAGVAAAGLALV
                     FVWSNRPAWDRLALTSGGHVYFKPAFVGPATRLLYLQEDTLGGITTVVENPRSSGPGS
                     TRTLLSNGKFQASDSGERLAQTGFALVPILHSTGRDDALVIGLGSGQTAEVVSRMGFR
                     RIDVAEIAPGIVEAADAYFDHINGKVLRRPGVRLRLEDGRNHLLLTDRAYDLIAIEIS
                     SIWFAGSTSLYCEEFFELTRRRLRPGGVLQQWVQVHHLGMDEIGSIVATMRRVYPHVS
                     FWLVGGQGILVASVEPQRLQPGSLEAAVRTNPWRENSPLATSERLVSLLCSRLLSSAD
                     VDRFLASRPFRVNTDANRYLEYATPRYNLDPRPLDAMNRNTLALFARFEGQEVAPGPA
                     SEVSDLLIRVTPERQREVLGLRRPSPGSP"
     gene            complement(1797..2741)
                     /locus_tag="PLB01_15265"
     CDS             complement(1797..2741)
                     /locus_tag="PLB01_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78712.1"
                     /translation="MKMFKAPLCGVLLLLFAALAPRASACSICRCGDPTFNALGSAIY
                     EPGKFRLALDWERFDKENGVSDPDTGEITGRDAEVENRFSLSAAYSFGERLTLVGRLP
                     VSVRRLTSSDADGATTTNTTGLSDPDFTALFRVWASDAVPGLGRRAWVSLVVGVKTRW
                     GRNDLSENGVRLDEHAQSGTGATDVYGGLSAAVLLDPQSSVFASFQYRRTGTNDYGYQ
                     YGRTTTANFAYERKLGRVVDAILEVNWRHAEQDVVDADGNRDPNTGGDLVYLTPRVVL
                     DLGGGILARAGVQIPVVKSLYGDQTERANVNIGLTMLF"
     gene            complement(2784..3158)
                     /locus_tag="PLB01_15270"
     CDS             complement(2784..3158)
                     /locus_tag="PLB01_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78713.1"
                     /translation="MQRVKALSAALLLALVAAAAVLPLVAAGAARGGCHCAVRMACCE
                     DGTCMMGGDEQPADGPEWRTCRREAPAGSMNPLDSYERALKSFFEWKRASATASLDDI
                     PVAPGRAASSCPATPPPRLFSF"
     gene            complement(3183..5291)
                     /locus_tag="PLB01_15275"
     CDS             complement(3183..5291)
                     /locus_tag="PLB01_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ78714.1"
                     /translation="MKKALLLLLCATLAPAASGEEYAAPEDAASKSKVKADVKVTAPR
                     PIRDLSDPDVRPDDLVGVADSAGTGIVTADRVAAREVHRAGDILEAVPGLLTSQHSGE
                     GKANQYYLRGFNLDHGTDVASAVAGVPVNMPTHAHGQGYSDNNFLIPELVSGIQYKKG
                     PYFADEGDFSAAGAVNVNVVNALDRPIVDLSFGGGGYGRGLFAGSVAAAGGTLLGALE
                     LYHNDGPWTTPDDFRKINGFLRWSTGAKGNGFSVTGVLYQGVWNSTDQVAQRGVDEGL
                     YGRYDAIDPTDGGRTHRYSLSADWETTGQGSRTRAQAYVMDYALDLWNNFTYFLNDPV
                     NGDQFQQVDRRVVTGGKVSHGRLLTLFGADAELTAGIDFRNDNIPTLGLLNTRARDLV
                     SIVRLDHVSQTSGALWAQAAVQVAPWFRAVAGLRGDLYRFNVASNVAENSGTETSGIV
                     SPKLSLVLGPFSNTEIYLNGGYGFHSNDGRGATLHVDPVTKDPVEPVTPLVRAKGAEI
                     GVRTTAIRGLQSTLGVWLLDIGSELVFSGDAGITEPSRPSRRIGFEWANYWSPFPWMT
                     VDADFAYSRARFTEDDPVGNYIPGAIEGVASAGVSVNNLEGFFGSVRLRYFGPRALIE
                     DDSVRSKASTLVNLKLGWQATTWARLTLDVYNLFNTQASDIDYYYTSRLPGEGAKGID
                     DVHFHPVDSRTIRAGLTLTF"
     gene            complement(5439..5861)
                     /locus_tag="PLB01_15280"
     CDS             complement(5439..5861)
                     /locus_tag="PLB01_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78715.1"
                     /translation="MAVVASMRSRIAALVGALLWSLGTIVLPALHLARHARPHDHEGG
                     GIHYHLDAEAYHDGHDDHDDDDEPGGPFTVEAPHGRRHAPHAAGLAHFAAATGEGAAV
                     VLAFTSEPDPERVPDALPGRCAEYFLEGQKAARAPPPA"
     gene            complement(5876..>6499)
                     /locus_tag="PLB01_15285"
     CDS             complement(5876..>6499)
                     /locus_tag="PLB01_15285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQQ78716.1"
                     /translation="DIVARYGGDEFVVLLPDTHLDQAALLAERLRAAVRTHADVGIST
                     GVAAALVDYRRAMGDAAGEARAKKAIADLDAKLPEDHRLVHRPLALYFSDHGEPARGL
                     AMTERELRGRRDVYGYDAYAWALYRAGRPAEAVEPMKLALSRGTVDPLLEFHAGAIAR
                     AAGDAAGARRHLERALKIDPRFHVLYAEEARQMLGGIARPAALASRR"
BASE COUNT          931 a         2224 c         2320 g         1024 t
ORIGIN      
        1 gagcgcatcg cgttcctgct cgccgtcggg ctgttctcgg ggttcctgtt cttcggtctc
       61 gaggtcgtct ggacgcacct catcggcgcg gtgctcggca acagcatcta cgcgttcgcg
      121 gccatgctcg cgctcgtgct ggcgggcctc gggatcggcg gcgctctgtc gacgcggctg
      181 ctgcgcgagg agcggccgat ctcgggcggg gccctcggcg cgatgctcct ggccggcagc
      241 ctgcttctcg cctggcagtt cacgctctgg ccccgcatcc cgaacgagtt catccgatgg
      301 ggaggaggcc tgaccacgtt cgcccaggga gagatgctgc gctgggccca ggcggcgcgc
      361 ctgctgctgc cgccggcgat cgtgttcggc atggtgtatc cggcgctgtt ccgcatggag
      421 gcctttcccc tgcggcggcg cgcccgccac gccgccgcgc tgggcgcgat gaactccgtg
      481 ggctgcgtcc tgggcgccct cctgaccggt ttcttcctca tcccggcgct gggctccgag
      541 gccacgctga tcctgttcgg gacgctcgcg ctggccgccg gcgtcctcgt ggcgctggct
      601 cacgaccggg gctggcggcg ggcgggagtg gccgcggcgg ggctcgcgct cgtcttcgtc
      661 tggtcgaatc ggcccgcgtg ggaccggctg gcgctgacct cgggagggca cgtctacttc
      721 aagcccgcgt tcgtgggccc cgcgacgcgt ctcctgtacc ttcaggaaga caccctcggc
      781 ggcatcacga cggtcgtcga gaatccgagg agcagcggac caggctccac acgcacgctg
      841 ctcagcaacg ggaagttcca ggcgagcgac tccggtgaac gcctcgccca gaccggcttc
      901 gccctcgtcc cgatcctgca ttccacgggc cgggacgacg cgctcgtcat cggcctcggg
      961 tccgggcaga cggcggaggt cgtgagcagg atgggattcc gccggatcga cgtcgcggag
     1021 atcgccccgg gaatcgtcga ggccgcggac gcctatttcg accacatcaa cggaaaggtc
     1081 ctgcggcgac cgggcgtccg cctccgcctc gaggacggcc gcaaccatct cctgctcacc
     1141 gacagggcct acgacctgat cgcgatcgag atcagcagca tctggttcgc cgggtccacg
     1201 agcctctact gcgaggaatt cttcgagctg acgcgccggc gtctccggcc cgggggcgtc
     1261 ctccagcaat gggtccaggt tcaccacctc ggcatggacg agatcggctc gatcgtggcg
     1321 acgatgcgac gcgtctaccc ccacgtgagt ttctggctcg tgggagggca gggcatcctc
     1381 gtggcgagcg tcgagccgca gcgcctgcag cccggctcgc tcgaggccgc ggtccggacg
     1441 aacccctggc gggagaactc gccgctggcg acctcggaac ggctcgtgtc cctgctctgc
     1501 agcaggttgc tgagctccgc cgacgtcgac cgtttcctcg cgtcacggcc gttccgcgtg
     1561 aacaccgacg ccaaccgtta cctcgagtac gcgacgcccc gctacaacct cgacccgcgt
     1621 ccgctggacg ccatgaaccg gaataccctc gcgctcttcg cccgcttcga ggggcaggag
     1681 gtcgcgccgg gccccgcctc ggaggtctcc gacctgctga tacgggtcac gcccgagcgg
     1741 cagcgggagg ttctcggact acggcgaccg tcgcccggct ccccgtagcg aatggactag
     1801 aagagcatgg tcaatccgat gttcacgttc gcgcgctccg tctggtcgcc atagaggctc
     1861 ttgacgacgg gaatctggac gccggcgcgg gcgaggatcc cgccgccgag gtcgagcacg
     1921 acgcgcggcg tgaggtacac gaggtcgccg cccgtgttcg ggtcgcggtt cccgtccgcg
     1981 tccacgacgt cctgctcggc gtggcgccag ttcacctcga ggatggcgtc gacgacgcgg
     2041 ccgagcttgc gctcgtacgc gaagttggcg gtggtcgtcc ggccgtactg gtagccgtag
     2101 tcgttcgtcc ccgtccggcg gtactggaac gaggcgaaaa cggaggactg cgggtcgaga
     2161 aggacggcgg cggagaggcc cccgtacacg tcggtcgccc cggtgcccga ctgcgcgtgc
     2221 tcgtcgaggc gcacgccgtt ctcggagagg tcgttccggc cccaccgcgt cttgacgccg
     2281 acgacgaggg acacccacgc gcggcgcccg aggccgggaa ccgcgtccga ggcccagacg
     2341 cggaagagcg cggtgaagtc ggggtccgag aggccggtcg tgttcgtcgt cgtcgccccg
     2401 tcggcgtcgc tcgacgtgag ccggcgaacg ctcaccggca accggccgac gagcgtgagc
     2461 cgctccccga acgagtacgc ggcgctcagc gagaatcggt tctcgacctc ggcgtcgcgg
     2521 cctgtgatct cgcccgtgtc cggatccgag acgccgttct ccttgtcgaa gcgctcccag
     2581 tccagggcga ggcggaactt tcccggctcg tagatcgcgg agccgagcgc gttgaacgtc
     2641 gggtcgccgc agcggcagat ggagcacgcg gaggcgcgcg gcgcgagcgc cgcgaaaagc
     2701 agcagcaaca ccccgcagag cggggccttg aacattttca agatgcagtt ctccttctga
     2761 ggaaattcgg agagaagaat ttcttagaaa gagaagagcc ggggaggagg cgtggcgggg
     2821 caggagctgg ccgcgcgccc gggggcgacg ggaatgtcgt cgagcgaggc cgtggcgctc
     2881 gctctcttcc attcgaagaa actcttcagc gccctctcat acgaatcgag ggggttcatc
     2941 gagcctgccg gcgcctcgcg ccggcaggtg cgccactcgg gcccgtcggc cggctgctcg
     3001 tccccgccca tcatgcaggt cccgtcctcg cagcacgcca tccgcacggc gcagtggcag
     3061 cctcctcgcg ccgcgcccgc ggccacgagt ggcagcaccg ccgcggccgc cacgagggcg
     3121 aggaggagag cggcggagag cgccttcacg cgctgcatcg gatgcggatt ctaacggcct
     3181 cgtcaaaacg tcagcgtcag ccccgctcga atcgtccgcg agtcgacggg atggaagtgc
     3241 acgtcgtcta ttcccttggc gccctcgccg gggaggcgcg acgtgtagta gtagtcgatg
     3301 tcgctggcct gcgtgttgaa gaggttgtag acgtcgagcg tgaggcgcgc ccacgtcgtc
     3361 gcctgccagc cgagcttgag gttgacgagc gtcgaggcct tcgaccggac gctgtcgtcc
     3421 tcgatcagcg cgcgcgggcc gaagtagcgc agacgcacgc tgccgaagaa gccctcgagg
     3481 ttgttgacgg agacgcccgc cgacgcgacg ccttcgatcg cgcccgggat gtagttcccg
     3541 acgggatcgt cctcggtgaa gcgcgcgcgc gagtatgcga agtcggcgtc cacggtcatc
     3601 caggggaagg ggctccagta gtttgcccat tcgaagccga tgcggcggct cggtcggctg
     3661 ggctccgtga tcccggcgtc gcccgagaag acgagctccg acccgatgtc gaggagccag
     3721 acgccgagcg tcgactggag gcccctgatc gcggtggtac gcacgccgat ttcggctccc
     3781 ttcgcgcgca cgaggggcgt cacgggctcg acggggtcct tcgtcacggg atcgacgtgg
     3841 agcgtcgcac cgcggccgtc gttgctgtgg aagccgtaac cgccgttcag gtagatctcc
     3901 gtgttcgaga acggcccgag gacgaggctg agcttcgggc tcacgatgcc gctggtttcc
     3961 gtgcccgagt tctcggccac gttgctcgcc acgttgaacc ggtagaggtc gccgcgcagg
     4021 cccgcgaccg cgcggaacca cggggcgacc tggacggcgg cctgcgccca cagggcgccg
     4081 ctcgtctgcg agacgtggtc gaggcggacg atcgagacga ggtcgcgggc tctcgtgttc
     4141 aggaggccga gcgtcgggat gttgtcgttg cggaagtcga tgccggccgt gagttcggcg
     4201 tcggcgccga agagcgtgag cagccgcccg tggctgacct tgccgcccgt gaccacgcgg
     4261 cggtccacct gctggaactg gtcgccgttc acggggtcgt tgaggaagta cgtgaagttg
     4321 ttccagaggt cgagcgcgta gtccatgacg tacgcctgag cgcgcgtccg cgagccctgg
     4381 cccgtcgtct cccagtccgc cgagaggctg tagcggtgcg tccgcccgcc gtcggtcggg
     4441 tcgatcgcgt cgtaccgccc gtaaagcccc tcgtccacgc cgcgctgcgc gacctggtcc
     4501 gtggagttcc agacgccctg atagaggaca ccggtcacgc tgaatccgtt gcccttggcg
     4561 cccgtcgacc agcgcaggaa gccgttgatc ttccggaagt cgtccggggt cgtccacggt
     4621 ccgtcgttgt ggtacagctc gagggcgccg agcagcgtgc cgccggccgc cgcgaccgag
     4681 cctgcgaaga ggccccgccc gtagccgccg ccgccgaacg agaggtctac gatcggcctg
     4741 tcgagcgcgt tgacgacgtt gacgttcacg gcgccggcgg ccgagaagtc gccttcgtcc
     4801 gcgaagtacg ggcccttctt gtactggatg cccgagacga gctcggggat caggaagttg
     4861 ttgtccgaat aaccctggcc gtgcgcatgc gtcggcatgt tcacgggcac gccggcgacc
     4921 gccgaagcga cgtccgtgcc gtggtcgagg ttgaagccgc gcaggtagta ctggttcgcc
     4981 tttccctcgc ccgagtgctg gctcgtcagg agcccgggca cggcctcgag gatgtcgccg
     5041 gcgcggtgca cctcgcgcgc cgccacccga tccgcggtga cgatgccggt gcccgcggag
     5101 tccgcgacgc cgacgaggtc gtcgggccgc acgtcggggt ccgagaggtc gcggatcgga
     5161 cgaggggccg tgaccttcac gtcggccttc accttcgact tcgaagccgc atcctcgggc
     5221 gcggcgtact cctcgccgga cgcagccggc gcgagggtcg cacagagcag cagcagcagc
     5281 gccttcttca cgtcctcgca cgggcacctt cgaagaggat cttcggattt cttcgaacga
     5341 aagaaaacac gggcagcctt cctttcgatg gcgaggtcgt tgcacgctca tccctccgaa
     5401 gaagggcggg tgcaagcaga cgcgggctcg atcgagcgtc aggccggggg cggagcgcgg
     5461 gcggctttct gaccttctaa gaagtattcg gcacaacggc ccggaagagc gtcgggaacg
     5521 cgctccgggt ccggctcgct cgtgaacgcc agcaccacgg ccgcgccctc gcccgtcgcc
     5581 gccgcgaaat gcgcgaggcc ggcggcgtgc ggcgcgtgac ggcggccgtg cggagcctcc
     5641 acggtgaacg ggccgccagg ctcgtcgtca tcgtcatgat cgtcatgtcc gtcgtggtac
     5701 gcctctgcgt cgaggtggta gtggatgccg ccgccctcgt ggtcgtgggg ccgcgcgtgg
     5761 cgagcgaggt ggagcgcggg aaggacgatc gtgccaagcg accacagaag ggcgcccaca
     5821 agggcggcga tcctcgagcg catcgacgcg acgacagcca tgccttcgaa gaatcctacc
     5881 ttcgactcgc gagcgcggcg gggcgcgcga tgccgccgag catctgcctc gcctcttccg
     5941 cgtaaagcac gtggaagcgc gggtcgatct tcagagcgcg ctcgaggtgg cggcgggctc
     6001 cggccgcgtc gccggcggcg cgcgcgatgg ctcccgcgtg gaactcgagg aggggatcga
     6061 cggtcccgcg cgacaaggcc agcttcatcg gctccacggc ttcggcgggg cgcccggcgc
     6121 ggtagagcgc ccaggcgtac gcgtcgtacc cgtagacgtc cctgcggccc ctgagctccc
     6181 gctccgtcat cgcgaggccg cgcgcaggct cgccgtggtc cgagaagtag agcgcgagcg
     6241 gacggtgaac gaggcggtgg tcctcgggca gtttcgcgtc gaggtcggcg atcgccttct
     6301 tcgcgcgcgc ctcgcccgcg gcgtcgccca tcgcgcggcg atagtccacg agcgcggccg
     6361 ccacgccggt gctgatgccc acgtcggcgt gcgtccgcac ggcagcccgc aggcgctcgg
     6421 ccagcagggc tgcctgatcg aggtgggtgt ccggcagcag cacgacgaac tcgtcgcctc
     6481 cgtagcgcgc gacgatgtc
//
LOCUS       DAOMFY010000230         6474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_3946_length_6474_cov_6.737498, whole genome shotgun sequence.
ACCESSION   DAOMFY010000230 DAOMFY010000000
VERSION     DAOMFY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3946_length_6474_cov_6.737498"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PLB01_15290"
     CDS             <1..1142
                     /locus_tag="PLB01_15290"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HQQ78717.1"
                     /translation="LVALFAVAAGAPEPRLSVLAAGLAVLAPLAWGFWRRSGAPWQEV
                     ALFAAVLWLLFVLHPVVLGRRAGRGRAPYLAAVLASAGFFPILRVALEEGGYRPVIGL
                     LPLAMALGMAGLVARLLTLGIPPEEDRSRLALVAGAALAFLTVAIPLQFEKEWLTLGW
                     AFLGAALAWLYGRIPHRGLVVWSLGLFAAAFVRLALNPAVLTYHARTATPVFNWYLWV
                     YAAAAAAFFVGAALLRGPGAAAWPVGVALLPAGGVALLFVLLNLEIADLFAEGRRLVV
                     NPFSTSLAEGLAYTLGWALFAIALLVAGIARRSRGARAAALVLLVVTILKCFLFDLAQ
                     LGGLYRVGSFVGLAVCLALVALLLQRFVLRREGPGVSTPAPGSSG"
     gene            complement(1111..4335)
                     /locus_tag="PLB01_15295"
     CDS             complement(1111..4335)
                     /locus_tag="PLB01_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78718.1"
                     /translation="MKNARRTFRRAVISVTNIAPLLAALLAAHAAQAGLRFQQVPTLP
                     GATATALASDGTNVWAGTPGGVWKLASGAWAPDGLAGQNVLSLSSGASVWAADGVKTW
                     RRGADGTWTAETLPASVTQPSALATDGTSLWAAGFGVAKKTGGAWAALASPGGKVLSA
                     TVWNGDLVAGLRGNVARYAGASVSYLSAGMPVTANVQALASVNGVLWAGTDQTLYSWS
                     GAAWVAEPGFGFHDVRAITGAGGLLRAATADAGVLAKSGAWSADNSGVLSPGALSFAA
                     AGADVYVGTSGAPVYRRVGSGWVEAGTGLWAASISDVMSLGGSSGTFASSHGAGLGEI
                     ASTSGGAAGAGCGDVSALAAAPGGFLAATNCDVTSFLLSGSSIVGSSSASAGLPLGVR
                     PTTLARVPADGSVAGGTPSAGMWRFVGSSWSADNGGLSGTEAILTMRSVGGALYASTG
                     TALFTRRPSGWAGATGAPSLVQALGGDTATLFAAPATGIAAATLGGPLPAAWRADDFG
                     ANTAFVSSLDAGNGMAFAAGGGAGVLRKKDGGWQPESTGLAAGADARVVRTLGSTLYA
                     GTAANGLFAAITVPAARTIPVVLDVTGATGARFRSELTIGNTGSQAGPWRVTFTGGGV
                     SVATSLTVPARTEIRAADALDFLRSLGLAIPPAPVTGSLTFDSGDTAGLYALTRTYSR
                     DTSGSYGVFLDAPTDLDAAEDAGFIYGLRSVAGVSRSNLAFASLPGRGSDPVTLSVQV
                     YDQGGAAAGAPIVVTLGPGEWKQLNGVLLTAGMTEPAYGYAKITRTSGVNAWTGYGVV
                     NDAKTSDGSILPLYRPGGLAAARRLVVPVVLDVFGAAGSHYTTELTLANDGAFATPVD
                     LFYKPALGSSTGVPFVTLTLAAGQQTTLPDVIAYLRTKGLNVPDASTGPQAGTLTVEF
                     RNLFNLDAPRTVAIARTTTPNPNAATGGAFGVAYPAAAKGGGARASALVPGLTRDASV
                     RSNLAVVHLGGGSESALSLSVQLYDAATGQPVGGPVTVSLLPGDWTQWSGIFDTAGVP
                     ANVTQAYAVVTRIAGDDTWLAYGVLNDAQTSDGSFIRMIPEQEY"
     gene            4417..5622
                     /locus_tag="PLB01_15300"
     CDS             4417..5622
                     /locus_tag="PLB01_15300"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQQ78719.1"
                     /translation="MAAVERGDAGSVSEELEKEEKRRFSSSGRGGEGAAAEIAAFAIS
                     TQGLSALVAGTRGASTLPAPVSDFLLAEISFSGARARRLQETLESVVLALRAAGVEVV
                     ALKGAVLASFHYPDPALRPMGDLDLLLKDPRDLARATEALGGAGWRALLDTPRHRVFA
                     RPDERVVRPAAEDPDNPIRVEIHTSFRLPVLGRAYDASRELREEAETRDIGGTRVNVA
                     AGSALLTHLLFHAAEDFAGNGIRGIQAYDFRLLASAREGERKTGALSPEISSSGRGRG
                     DGGSAAPLLYAADAIERLFPLTFKEVFLSSLSSGVPGELRRRAAALPPLRYTRPARGW
                     TRTLLSLIDARAPRARFLLRILFPTLGEVKANAAPNSSGISLVAVYARVFGRRVKSAL
                     RGLFSRVAG"
     gene            complement(5606..>6474)
                     /locus_tag="PLB01_15305"
     CDS             complement(5606..>6474)
                     /locus_tag="PLB01_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78720.1"
                     /translation="LTVFTATVYGDVARLWHACVTRALGDAARIEIFDDSPGQDLDAE
                     FFPGAEILRRSPSRRDFHEAYNDAIRRCGTPFLAFVDSDILWLDTGAWKRAAAELEDP
                     RVAAVSCVSRAKTESHGTFAVVMRAEDYRCALARLPGGFFPAIAKLNPEIPKERWTWH
                     DTGDLMTRAVVSSGSDVRLLNLERKGEFARFDGITLSRRAALWMGEAALAKLSRENDY
                     FWHGWIGNATLKSLHDAAFPDGPRYAFPFSRAAALVHAVSPSPRRTRDRLSYLLRLRR
                     QAAAVRALIRRP"
BASE COUNT          817 a         2346 c         2379 g          932 t
ORIGIN      
        1 tcctcgtcgc gctcttcgcc gtggccgcgg gcgcgcccga gccgcgcctc tccgtgctcg
       61 cggcggggct cgcggtcctc gcgccgctcg cctggggatt ctggcgccgg agcggcgcgc
      121 cgtggcagga ggtcgcgctg ttcgcggccg tcctctggct gctgttcgtc ctccatcccg
      181 tggtgctcgg gcggcgcgca ggccgaggcc gggcgccgta tctcgcggcg gtcctcgcga
      241 gcgccggttt cttcccgatc ctgcgggtcg cgctcgaaga gggcgggtac cgcccggtga
      301 tcggtctcct tccgctcgcg atggcgctcg ggatggccgg gctcgtcgcg cggctcctga
      361 ccctcggaat cccgccggag gaggatcgct cgcgcctcgc gctcgtcgcg ggcgcggcgc
      421 tcgcgttcct gaccgtcgcg atcccgctgc agttcgagaa ggagtggctg acgctcggct
      481 gggcgttcct cggcgccgcg ctcgcgtggc tgtacggccg gattccccac cgcggcctcg
      541 tcgtgtggtc cctcgggctc ttcgcggccg cgttcgtgag gctcgccctc aatcccgccg
      601 tcctgacgta ccacgcgcgt acggcgacgc ccgtcttcaa ctggtatctc tgggtctacg
      661 cggccgcggc cgcggccttc ttcgtcggtg cggccctgct gcggggcccg ggcgcggcgg
      721 cgtggccggt cggcgtcgcc ctcctgcccg cgggcggggt tgcgctcctc ttcgtcctcc
      781 tgaacctcga gatcgcggac ctctttgcgg aggggcggcg gctcgtcgtg aacccgttct
      841 cgacgtcgct cgccgagggc ctcgcctaca cgctcggctg ggcgctgttc gcgatcgccc
      901 tgctcgtcgc gggaatcgcg cggaggagcc gcggagcgcg cgccgccgcg ctcgtcctcc
      961 tcgtcgtcac gattctcaag tgcttcctct tcgacctcgc gcagctcggc ggcctgtacc
     1021 gggtcggctc gttcgtcggc ctcgcggtct gtctcgcgct cgtcgcgctg ctcctgcagc
     1081 ggttcgtcct gcggcgcgag ggcccgggcg tcagtactcc tgctccggga tcatccggat
     1141 gaagcttccg tcggacgtct gggcgtcgtt caggacaccg tatgcgagcc acgtgtcgtc
     1201 gcccgcgatg cgcgtcacga cggcgtacgc ctgcgtcacg ttcgccggga cgcccgcggt
     1261 gtcgaagatc cccgaccact gcgtccagtc gccggggagg agcgagacgg tgacggggcc
     1321 tccgacgggc tgccccgtcg cagcgtcgta gagctggacg gagagcgaga gcgccgactc
     1381 cgagccgccg ccgaggtgca cgacggcgag gttcgagcgc acggacgcgt cgcgcgtgag
     1441 gcccgggacg agcgcggagg cgcgcgcccc gccgcccttc gcggcggccg gatatgcgac
     1501 gccgaacgcg ccgccggtcg cggcgttcgg attgggcgtc gtcgtccggg cgatcgcaac
     1561 cgtgcgcggc gcgtcgaggt tgaagaggtt gcggaactcg accgtgagcg tcccggcctg
     1621 cggccccgtc gaggcgtcgg gcacgttcag acccttcgtc cggaggtacg cgatcacgtc
     1681 cgggagcgtc gtctgctggc cggccgcgag cgtgagcgtc acgaacggga cgcccgtcga
     1741 ggacccgagc gccggcttgt agaagaggtc gacgggcgtc gcaaacgcgc cgtcgttcgc
     1801 gagcgtgagc tcggtcgtgt agtgcgagcc ggccgcgccg aagacgtcga ggacgaccgg
     1861 cacgacgagc cgccgcgccg cggcgaggcc gccggggcgg tagagcggaa ggatcgagcc
     1921 gtcggacgtc ttcgcatcgt tcacgacgcc gtagcccgtc cacgcgttga cgccggacgt
     1981 gcgcgtgatc ttcgcgtacc cgtacgcggg ctccgtcatt ccggccgtga ggaggacgcc
     2041 gttgagctgc ttccattcgc cgggaccgag cgtgacgacg atcggcgctc cggccgccgc
     2101 gccgccctgg tcgtagacct ggacggagag cgtgaccggg tccgagccgc gccccggcag
     2161 ggaggcaaac gcgaggttcg accgcgaaac gcccgcgacg ctccggaggc cgtagatgaa
     2221 tcctgcgtcc tcggcggcgt cgaggtcggt cggggcgtcg aggaagacgc cgtaactgcc
     2281 gctcgtgtcg cgcgaatacg tccgcgtcag cgcgtagagc ccggcggtgt cgccggagtc
     2341 gaacgtcagc gaacccgtga cgggcgccgg cgggatcgcg aggccgaggc ttcgcaggaa
     2401 gtcgagcgcg tcggccgcgc ggatctcggt gcgcgccggc acggtgagcg acgtcgcgac
     2461 gctcacgccg ccgccggtga acgtcacgcg ccacggcccg gcctgggacc cggtgtttcc
     2521 gatcgtgagc tcggaccgga accgcgcgcc cgtcgcgccc gtgacgtcga ggacgacggg
     2581 aatcgtccgg gccgcgggaa ccgtgatcgc cgcgaacagc ccgttcgctg ccgttcccgc
     2641 gtaaagcgtc gacccgaggg tgcgcacgac gcgcgcgtcg gcgcccgccg cgagacccgt
     2701 gctctcgggc tgccagccgc cgtccttctt gcgcaggacg cccgcgccgc cgccggccgc
     2761 gaaggccatg ccgtttcccg cgtcgagcga cgagacgaag gccgtgttgg cgccgaagtc
     2821 gtcggcgcgc cacgcggcgg ggagcgggcc tccgagcgtc gcggccgcga ttcccgtggc
     2881 cggagctgca aagagcgtcg cggtgtcgcc gccgagagcc tgaacgagcg acggcgcgcc
     2941 cgtcgcgccg gcccagccgc tcggcctgcg cgtgaagagg gccgtgccgg tcgaggcgta
     3001 gagcgctccg ccgacggagc gcatcgtgag gatggcctcc gtacccgaga ggccgccgtt
     3061 gtccgccgac cacgacgatc ccacgaatcg ccacatcccg gcgctcggcg tgccgcccgc
     3121 aacgctgccg tcggccggga cgcgcgcgag cgtcgtcggc cggacgccga gcggaaggcc
     3181 cgccgaggcg ctcgaggaac ccacgatcga ggagcccgag agaagaaacg acgtgacgtc
     3241 gcagttcgtg gccgcgagaa agccgcccgg ggccgccgcg agggcgctga cgtcgccgca
     3301 gcccgctccc gccgccccgc ccgacgtcga tgcgatctcg ccgaggcccg caccgtgcga
     3361 ggacgcgaag gtcccggatg atcccccgag gctcatcacg tccgagatgc tcgcggccca
     3421 gaggcccgtg cccgcctcga cccagcccga gccgacgcgg cggtagaccg gcgctccgga
     3481 ggtcccgacg tagacgtcgg cgcccgcggc ggcgaagctc agcgccccgg gcgagaggac
     3541 gccggagttg tccgcggacc acgcgcccga cttcgcgagc acgcccgcgt cggccgtcgc
     3601 ggcccgcagg aggccgccgg cgcccgtgat cgcacgcacg tcgtggaagc cgaagcccgg
     3661 ctcggcgacc cacgcggcgc cgctccacga gtagagagtc tggtccgtgc cggcccagag
     3721 gacgccgttc acggacgcga gcgcctgaac gttcgccgtg accggcattc cggccgacag
     3781 gtacgagacg gaggctcccg cgtagcgcgc gacgttgccg cgcaggcccg cgacgaggtc
     3841 gccgttccag acggttgcgg agagcacctt gccgcccggg ctcgcgagcg cggcccatgc
     3901 gccgccggtc ttcttcgcga cgccgaatcc cgcggcccag aggctcgtcc cgtcggtcgc
     3961 gagggcggag ggctgcgtga cggaggcggg cagcgtctcg gccgtccagg tgccgtcggc
     4021 gccgcgcctc caggtcttca ccccgtcggc ggcccacacg gacgcgccgg acgaaaggga
     4081 gagcacgttc tgccccgcga gcccgtccgg cgcccatgct ccggacgcga gcttccagac
     4141 gcctcccggc gtgcccgccc acacgttcgt gccgtcggag gcgagcgccg tcgcggtcgc
     4201 gcccgggagc gtcggcacct gctggaaccg gagcccggcc tgcgccgcgt gggcggcaag
     4261 gagcgccgcg aggagcggag cgatgttcgt gacggagatc acggcgcggc ggaaggtgcg
     4321 tctggcgttc ttcacgtcgg agagaatagc ggcattctct tcgagcccga acgcctcttt
     4381 gcccatcccc gttacgccgc attcgccgcg gcggcggtgg cggccgtcga gcggggggac
     4441 gcggggtccg tctccgagga gctggagaaa gaagagaaga ggaggttttc ttccagtgga
     4501 agagggggag agggtgcagc ggcggagatc gcggcctttg cgatctcgac gcagggtctc
     4561 tcggcgctcg tggccggcac ccgcggcgcc tcgacgctcc ccgctcccgt ctccgatttt
     4621 ctactcgcag aaatctcctt ctccggtgcc cgcgctcggc gcctccagga gacgctcgag
     4681 tccgtcgtcc ttgcgctgcg cgccgcgggc gtcgaggtcg tcgcgctcaa gggcgccgtc
     4741 ctcgcgtcct tccactaccc ggaccccgcg ctccgcccga tgggcgacct cgatctcctc
     4801 ctcaaggacc cccgcgatct cgcgcgcgcg acggaggcgc tcggcggcgc cggctggcgc
     4861 gccctcctcg acacgccgcg ccaccgcgtc ttcgcacgcc cggacgagcg cgtggtccgt
     4921 cccgccgccg aggatccgga caacccgatc cgcgtcgaga tccacacgtc gttccgcctg
     4981 cccgtcctcg ggcgcgctta cgacgcgagc agggaactgc gcgaagaggc cgagactcgc
     5041 gacatcggcg gtacgcgcgt gaacgtcgcc gcgggaagcg cgctcctcac gcacctcctc
     5101 ttccacgccg cggaggactt cgcgggaaac ggcatccggg ggatccaggc gtacgacttc
     5161 cggcttctcg cgagcgcgag ggagggagag cggaagaccg gcgctctctc gccggagatt
     5221 tcttcgagtg gaagagggag gggagacggt ggctcggcgg cgccgcttct ctacgcagcc
     5281 gatgcgatcg agcgcctctt ccctctaact ttcaaagaag tcttcctctc ttctctttct
     5341 tccggtgtcc ccggggagtt gcgccgccgc gcggcggcgc tgccgccgct ccgctacacg
     5401 cgtcccgctc gcggctggac gcgcacgctc ctctcgctga tcgacgcgcg ggccccgcgc
     5461 gcgcgctttc tcctgcggat cctcttcccg accctcggcg aggtgaaggc gaacgcggcg
     5521 ccaaactcct cgggaatctc gctcgttgcc gtgtacgcgc gcgtcttcgg gcgtcgggtg
     5581 aagtccgcac tccgcgggct gttctctagg gtcgccggat gagcgcacgg acggctgccg
     5641 cctgccggcg gaggcgcaga aggtaggaga gccggtcgcg ggtgcggcgc gggctcggcg
     5701 agaccgcgtg gacgagcgcg gccgcgcgcg aaaacgggaa ggcgtaccgc gggccgtccg
     5761 ggaaggcggc gtcgtgcagc gatttcagcg tcgcgttgcc gatccacccg tgccagaagt
     5821 agtcgttctc ccgtgagagt ttcgcaagcg cggcctctcc catccacagc gcggcccggc
     5881 gcgagagcgt gatgccgtcg aagcgcgcga actccccttt ccgttcgagg ttcaggaggc
     5941 gcacgtcgct tccggaggag acgacggcgc gcgtcatgag gtctcccgtg tcgtgccacg
     6001 tccagcgctc cttcggaatc tcgggattca gcttcgcgat cgccggaaag aaccccccgg
     6061 ggaggcgggc gagcgcgcag cggtagtcct ccgccctcat gacgacggcg aaggtgccgt
     6121 ggctctcggt cttcgcgcgg gacacgcagg aaaccgcggc gacgcgcggg tcctcgagct
     6181 cggcggccgc gcgtttccat gcgccggtgt ccagccagag gatgtcgctg tcgacgaacg
     6241 ccaggaacgg cgtcccgcat cggcggatcg catcgttgta ggcctcgtgg aaatcgcgcc
     6301 gcgagggcga gcgccggagg atctccgcgc cggggaagaa ctcggcgtcg aggtcctggc
     6361 cgggcgagtc gtcgaagatc tcgatccgcg ccgcgtcgcc gagcgctcgg gtgacgcagg
     6421 cgtgccagag gcgcgcgacg tcgccgtaga cggtcgccgt gaagacggtg agcg
//
LOCUS       DAOMFY010000231         6417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4001_length_6417_cov_7.186262, whole genome shotgun sequence.
ACCESSION   DAOMFY010000231 DAOMFY010000000
VERSION     DAOMFY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4001_length_6417_cov_7.186262"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PLB01_15310"
     CDS             complement(<1..1413)
                     /locus_tag="PLB01_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78721.1"
                     /translation="MTTRGAAAGTGRSFPAFAWTLALGLPALLAVHALSFGRIPSGLE
                     GGPAVEALRGVHLVVRRRFDVLSFAIGPSAETLWLYVMGGSVELFGPRWGSVVFPSVL
                     ASATVVFLTVLAARRLDPDTPPAIPFLLSAGSVWLFHYGQVGLRAISAPLFFLLAVLL
                     VHAGAAAGATWRVRFAAGFVLGLSVYAYSSCRVLAPAWLLWAAAGELKKPRAERAWRA
                     ALGPALAGIAVASIPNVLFFLREPGEFLFRGYYVYRGGWTWKVVNVFWTALLPFHLPR
                     FYTVWLGPGHNFDATAVSVTSAGVHPVDPVTAVAFACGLVLAARGPRPAGLSYLLWVL
                     GAGTLLLGVSGPSLTRLLMLQPAIVLLAALAVGRAWRRWPRARLALAAALLAPGALGF
                     AEYATLFGPSADAQSEYLAPANAMGARARDLLVVTFTDVTRQRDTEDSRVQLQFVVDS
                     LCELVAVLAPDGRIRLVNRAW"
     gene            complement(1466..2041)
                     /locus_tag="PLB01_15315"
     CDS             complement(1466..2041)
                     /locus_tag="PLB01_15315"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQQ78722.1"
                     /translation="MKRALVFAAVFAMAAGSAAAQNTTPAPMPDLVITSFGLASWGTS
                     CVKGQTLFTFSVGVKNQGTASWSGPNRPVVVVADMHLPNPDSFGTGVAIDPPLAPGET
                     RQVSVPITYYDADPAHIKAGAPHPFHATVNRDHKIAESNFANNDGPGPATWNGMKVIN
                     VGAPEACLKPAKPAPGRAAPPAPTPTLVPIR"
     gene            2224..3861
                     /locus_tag="PLB01_15320"
     CDS             2224..3861
                     /locus_tag="PLB01_15320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ78723.1"
                     /translation="MTLSRIPFHRRLGFKITATAAAAILVPVAIVAAVTIRAQHDELV
                     ARAQGSAAILSETVRSATHDFMLKDQREDAYRIVEIVGHESLREGSSIESVRIFNKQG
                     ATVYSTDRAEIGRIADKSAGACAPCHGGAGAPIANPDPATRSRISAVGDRRVLSFVTP
                     IYNEAACAAAGCHAAPKEQRVLGLLDVALSLRDVDDRRAAATWRIVGLSLLALVALTA
                     LVSAIVRVLVFRPVSDMLAATKDLAHGGTPAPLPIRSDDELGMLGRSFNEMSDSLRKA
                     RAELERAADGLERQVEERTAALQRAQEALVQSEKLVSLGTLSASIAHEINNPLAGILT
                     FARLMTRTLESGEVDDAARAACLKNLGLIQRETERCTRIVRSLLDFARARPIELHAVD
                     AVAALDEAVSLTQHKLQLEQVAVTKDLDGAAVVQGDFGQLRQVFVNLILNACDAMPGG
                     GTLTVTARKVDDGRTVEIALSDTGAGIAPEHLSRIFDPFFTTKQMGTGLGLSVAYGVV
                     EKHGGSMKAVSRVGAGTTMTIRLPLAGETAAPAGAAA"
     gene            3858..4439
                     /locus_tag="PLB01_15325"
     CDS             3858..4439
                     /locus_tag="PLB01_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HQQ78724.1"
                     /translation="MSEVLLLWIGVRDPAADLLEGVRRSVAREFEIDARVSVSAERPE
                     DAFDARRGQHSSTRILAWLQERREGAHRVLGVTDADLFIPILTFVFGEAQLGGRAAVV
                     STARLGDTPLIPGEPARIALRLQKEAVHELGHTFGLLHCADARCAMARSPSLRHVDEK
                     SPVLCRACRARTRELSENGDVHEQEEHANPDRR"
     gene            4402..5793
                     /locus_tag="PLB01_15330"
     CDS             4402..5793
                     /locus_tag="PLB01_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ78725.1"
                     /translation="MSKKNTRILIVDDEEIVRESLSGWLGKDGYTIGTAPDGLTALKL
                     MEEHPWTILLVDLKMPGIDGLEVLRRVKERWSDTAVLIMTAYATVDTAVNAMKAGAFD
                     YVVKPFDPEEVSLVVEKIVAQQALVRENVILRKALKKEYRFHDLVTKSPALLATLELA
                     KTAARSPSTVLVLGESGTGKELLARAIHAESPRSGKPFVAMSCAALTETLLESELFGY
                     EKGAFTGANGRREGKFEAASGGTLFLDEIGDISPKLQLDLLRVLEERRVTRVGGTDSL
                     PVDVRIIAATNRDLRRAVEEGRFRQDLFYRLNVITITLLPLRERKEDIPLLVDRLLEQ
                     LSSELKRPAERVSPEAMAILLAHSWPGNVRELRNVLERAIVVSEGPVLEARHLGLVLQ
                     DGAAAPVPEHPASLEEIERQYIGSVLKQSGGNVTQAARILDIDRVTLYAKIRKYGLKR
                     AEDGEFEPATPAR"
     gene            5944..6195
                     /locus_tag="PLB01_15335"
     CDS             5944..6195
                     /locus_tag="PLB01_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78726.1"
                     /translation="MMNDTTTACPWLKQVVMLYCDACPTKKMVPRDQLVSMGPCLAGS
                     FSECAMYREMVQRLEAGAARNDDETEASLAAASGREVPS"
BASE COUNT         1017 a         2275 c         2307 g          818 t
ORIGIN      
        1 ccacgcccgg ttgaccagcc ggatccgccc gtctggcgcc agcaccgcca ccagctcgca
       61 gagcgagtcg acgacgaact ggagctgcac ccgcgagtcc tcggtgtccc tctgccgggt
      121 gacgtcggtg aacgtgacca ccaggaggtc gcgcgcgcgc gcgcccattg cgttcgccgg
      181 agcaaggtac tcgctctggg cgtccgcgga cggcccgaag agcgtcgcgt actcggcgaa
      241 cccgagggcg ccgggcgcga gaagcgcggc cgcgagggcg agacgcgcgc gcggccacct
      301 ccgccacgcc cggccgaccg cgagggcggc gaggaggacg atcgcgggct gcagcatgag
      361 gagccgcgtg aggctcggtc ccgagacgcc gaggaggagc gtccccgccc cgagcaccca
      421 gagaagatag gaaagcccgg cgggccgagg cccgcgcgcc gcgaggacga ggccgcacgc
      481 gaacgcgacc gccgtgacgg ggtcgacggg gtgcacgccg gcgctcgtga cggagacggc
      541 cgtcgcgtcg aaattgtggc cgggcccgag ccacaccgtg tagaagcgcg gaaggtggaa
      601 aggcaggagc gccgtccaga agacgttcac gaccttccac gtccagccgc cgcggtacac
      661 gtagtagccg cggaagagga actcgccggg ctcccgcagg aagaagagga cgttcggaat
      721 cgacgcgacg gcgatcccgg cgagcgccgg gccgagcgcc gcgcgccacg cgcgttccgc
      781 ccgcggcttc ttcagctcgc ccgcggccgc ccagagcagc caggccgggg cgaggacgcg
      841 gcacgagctg taggcgtaga cggacaggcc gaggacgaag cccgccgcga accggacccg
      901 ccacgtcgct ccggccgctg ctcccgcgtg aaccaggagg acggcgagca ggaagaagag
      961 aggggcggag atcgcgcgca ggccgacctg cccgtagtgg aagagccaga cggaacccgc
     1021 cgacaggaga aacgggatcg cggggggcgt gtccgggtcg agccggcgcg ccgcgaggac
     1081 ggtgagaaag acgaccgtgg ccgaggcaag gacggacgga aagacgacgg agccccagcg
     1141 cggaccgaag agctcgacgg acccgcccat cacgtagagc cagagcgtct ccgcggaagg
     1201 cccgatcgcg aacgagagga cgtcgaagcg ccgccgcacg acgaggtgca cgccgcgcag
     1261 cgcttcgacg gcgggtccgc cctcgaggcc ggacgggatg cggccgaacg acagcgcgtg
     1321 gacggcgagg agagccggaa gccccagggc gagcgtccac gcgaacgcgg ggaaggagcg
     1381 gcccgtcccg gcggccgcgc cgcgcgtcgt catcgcggca ttctagaagc ggagcgggcc
     1441 ccgcggcccg ctccgccggt atgcctcagc ggatcgggac gagcgtgggc gtgggcgcgg
     1501 gcggcgcggc gcggccgggc gcgggcttcg cgggtttgag gcaggcctcg ggggcgccga
     1561 cgttgatgac cttcatgccg ttccaggtcg cgggccccgg cccgtcgttg ttcgcgaaat
     1621 tggattcggc gatcttgtgg tcgcggttca ccgtcgcgtg gaaggggtgc ggggctcccg
     1681 ccttgatgtg ggcgggatcg gcgtcgtagt acgtgatcgg aaccgacacc tgccgggttt
     1741 cgccgggcgc gagcggcggg tcgatcgcga cgccggtgcc gaacgagtcg ggattcggca
     1801 ggtgcatgtc cgccacgacg acgaccggtc ggttggggcc gctccagctg gcggtcccct
     1861 ggttcttcac gccgaccgag aacgtgaaga gggtctgacc cttcacgcac gaggtccccc
     1921 acgaagccag tccgaagctc gtgatcacga gatcgggcat cggggcgggc gtggtgttct
     1981 gtgcggccgc ggaacccgcg gccatggcga atacggcggc gaaaacgaga gctcttttca
     2041 cgagacctcc tgatgccgcc gaatgtaagg cctggcgggc ccggcgcaag gcccggccgt
     2101 ggctcccgga cgccagaacc gggtttcggg gcgtcacggc gccccgaaac ggccctccgg
     2161 cgcggccgaa tggttcaaat gagggatgga cggccctcgg ccgccccccg acattgacct
     2221 tggatgactc tgtcccggat tcccttccac cgccggctcg gcttcaagat cacggccact
     2281 gcggcggcgg cgatcctcgt gcccgtcgcg atcgtggcgg ccgtcacgat ccgcgcccag
     2341 cacgacgagc tcgtggcgcg cgcgcagggc agcgccgcga tcctctcgga gaccgtccgg
     2401 agcgcgacgc acgacttcat gctgaaggac cagcgcgagg acgcgtaccg gatcgtcgag
     2461 atcgtcgggc acgagagcct ccgcgagggc agctcgatcg agagcgtccg gatcttcaac
     2521 aagcagggcg ccaccgtgta ctcgaccgac cgcgccgaga tcgggcggat cgcggacaag
     2581 agcgccgggg cctgcgcgcc gtgccacggc ggggcgggcg cgccgatcgc gaaccccgac
     2641 cccgcgacgc gcagccggat ctcggcggtc ggcgaccggc gcgtcctctc gttcgtgacg
     2701 ccgatctaca acgaggccgc gtgcgcggcg gccggctgcc acgcggcgcc gaaggagcag
     2761 cgcgttctcg ggctcctcga cgtcgcgctc tcgctgcgcg acgtggacga ccggcgcgcc
     2821 gccgcgacgt ggcggatcgt cgggctgtcg cttctcgcgc tcgtcgccct gacggcgctc
     2881 gtctcggcga tcgtccgcgt cctcgtgttc cgtccggtct cggacatgct cgcggcgacg
     2941 aaagacctcg cgcacggcgg cacgccggcg cccctcccga tccggtcgga cgacgagctc
     3001 gggatgctcg gccgctcgtt caacgagatg agcgactcgc tgcgcaaggc ccgcgcggag
     3061 ctcgagcgtg ccgcggacgg cctcgagcgc caggtggagg agcggacggc cgcgctccag
     3121 cgggcgcagg aggcgctcgt gcagtccgag aagctcgtct cgctcgggac gctctccgcg
     3181 tcgatcgccc acgagatcaa caacccgctc gcgggcatcc tgacgttcgc gcgcctcatg
     3241 acgcgcacgc tcgagtcggg cgaggtcgac gacgcggcgc gcgcggcctg cctgaagaac
     3301 ctcgggctga ttcagcgcga gaccgagcgc tgcacgcgaa tcgtgcggag cctcctcgat
     3361 ttcgcgcggg cgcgcccgat cgagctccac gcggtcgacg ccgtcgccgc tctcgacgag
     3421 gccgtgtcgc tcacgcagca caagctccag ctcgagcagg tcgccgtgac gaaggatctc
     3481 gacggcgcgg cggtcgtcca gggcgacttc ggccagctgc ggcaggtctt cgtgaacctc
     3541 atcctgaacg cctgcgacgc gatgccgggc ggcgggacgc tcaccgtgac cgcgcggaag
     3601 gtggacgacg ggcgcaccgt ggagatcgcc ctctcggaca cgggcgcggg catcgcgccc
     3661 gagcacctct cgcggatctt cgacccgttc ttcacgacga agcagatggg cacgggcctc
     3721 ggcctctccg tcgcgtacgg cgtcgtcgag aagcacggcg gctcgatgaa ggcggtcagc
     3781 cgcgtcggcg cggggacgac gatgacgatc cggctcccgc tcgccgggga gacggcggcg
     3841 ccggcgggcg ccgccgcgtg agcgaggtcc tcctcctgtg gatcggagtt cgggacccgg
     3901 ccgcggatct cctcgaaggc gttcgccgga gcgtggcgcg cgagttcgag atcgacgccc
     3961 gcgtgagcgt ctcggccgag cgccccgagg acgcgttcga cgcgcgccgg ggtcagcact
     4021 cgtccacgcg gatcctcgcg tggctgcagg agcgccggga gggggcgcac cgggtcctcg
     4081 gcgtgacgga cgccgacctc ttcatcccga tcctgacctt cgtcttcggc gaggcgcagc
     4141 tcggcggccg cgccgcggtc gtctcgacgg cccgcctcgg cgacaccccg ctgatccccg
     4201 gcgagccggc gcgcatcgcc ctgcgcctcc agaaggaggc cgtgcacgag ctcgggcaca
     4261 cgttcggcct cctgcactgc gccgacgcgc gctgcgccat ggcgcgttcg ccgagcctga
     4321 gacacgtcga cgagaaatcc cccgtccttt gccgcgcctg ccgcgcccgc acccgcgaac
     4381 tctcggagaa cggagacgtc catgagcaag aagaacacgc gaatcctgat cgtcgatgac
     4441 gaggagatcg tccgggagtc cctctcgggc tggctcggca aggacggcta cacgatcggg
     4501 accgcgcccg acgggctgac ggccctgaag ctcatggagg agcacccgtg gacgatcctc
     4561 ctcgtggatc tcaagatgcc cggcatcgac gggctcgagg tcctgaggcg cgtcaaggag
     4621 cgctggagcg acacggccgt cctcatcatg acggcctacg ccaccgtcga cacggccgtc
     4681 aacgcgatga aggccggcgc gttcgactac gtcgtcaagc cgttcgaccc cgaggaggtg
     4741 agcctcgtcg tcgagaagat cgtcgcgcag caggcgctcg tgcgcgagaa cgtgatcctc
     4801 cggaaggccc tgaagaagga gtaccggttc cacgacctcg tcacgaagag cccggcgctt
     4861 ctcgcgacgc tcgagctcgc gaagacggcc gcgcgcagcc cgtccacggt cctcgtcctc
     4921 ggggagagcg gaaccggcaa ggagctcctc gcccgcgcga tccacgccga gagcccgcgc
     4981 agcggcaagc cgttcgtcgc gatgtcctgc gcggccctga cggagacgct cctcgagtcc
     5041 gagctcttcg ggtacgagaa gggcgcgttc acgggcgcga acggccggcg ggaagggaag
     5101 ttcgaagcgg cgagcggcgg cacgcttttc ctcgacgaga tcggcgacat cagcccgaaa
     5161 ctccagctcg acctcctgcg cgtcctcgag gagcgccggg tcacgcgggt gggcggcacg
     5221 gattcgctgc ccgtcgacgt ccggatcatc gccgcgacga accgggacct ccggcgggcg
     5281 gtcgaggagg gccgattccg gcaggatctc ttctaccgcc tgaacgtcat cacgatcacg
     5341 ctcctcccgc tgcgcgagag gaaggaggac attcccctgc tcgtcgaccg gcttctcgaa
     5401 cagctctcgt cggagctcaa gcgccccgcg gagcgcgtct cgcccgaggc gatggcgatc
     5461 ctcctcgccc actcgtggcc cgggaacgtc cgcgagctgc gcaacgtcct cgagcgcgcg
     5521 atcgtggtct ccgagggccc cgtcctcgag gcccggcacc tcggtctcgt cctccaggac
     5581 ggcgccgcgg cgcccgttcc cgagcacccg gcctcgctcg aggagatcga gcgccagtac
     5641 atcggaagcg tcctcaagca gagcggcggc aacgtgacgc aggccgcccg catcctcgac
     5701 atcgaccgcg tgactctcta cgccaaaatt cggaagtacg ggctgaaacg cgcggaagac
     5761 ggggagttcg aacccgcaac gcccgcccgc tgagaatcac aaccctccgt tgagaaaacg
     5821 aacgcgcggg cctcccgcgc gttctttcgt tcttcaaggg caagacgctg cgcccgtgcg
     5881 tgttgcaggc gcgacgagcc gccgcgcgaa gcgtggcccg gtccgtgcaa tccctccgtc
     5941 gccatgatga acgacaccac gaccgcctgc ccctggctga agcaagtcgt gatgctctat
     6001 tgcgacgcct gcccgacgaa aaagatggtc ccgcgcgacc agctcgtgtc catgggtccg
     6061 tgcctcgccg gctccttctc ggagtgcgcg atgtaccgcg agatggtcca gaggctcgag
     6121 gccggcgccg cccgcaacga cgatgaaacg gaagcgagcc tcgcggccgc ttccgggagg
     6181 gaggtcccgt catgatcgcc accgttccgc tcgccgttct cgccctcctg acgctcctcg
     6241 cgatggcccg ccagagccgg gtccacgccg cgtgagaagg agaaagacgc gatgaacctg
     6301 aaccgccgca accttctcaa gatcgccggc gccgccggtg cgggagccgc cctcaccggc
     6361 ggtgccctcg cgtcggccac gacgcgaggg gccgcgaagg acgccgtcgg cgttctc
//
LOCUS       DAOMFY010000232         6395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4020_length_6395_cov_6.616404, whole genome shotgun sequence.
ACCESSION   DAOMFY010000232 DAOMFY010000000
VERSION     DAOMFY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4020_length_6395_cov_6.616404"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLB01_15340"
     CDS             <1..492
                     /locus_tag="PLB01_15340"
                     /inference="COORDINATES: protein motif:HMM:NF014712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HQQ78727.1"
                     /translation="LDAVPSARTCLVLGGPGFDASPLAALEADPPAAPPSPALRAHEI
                     RFTPDGADLDEIASRCGLTPAEFLRAFTALTYTVGFLGFTPGFAYLYGLPLAFHLPRR
                     PGPRLAVPAGSIALAGPYVGIYPGATPGGWNLLGTTAQRLFDLRGDPPFLFSPGDTVR
                     FIA"
     gene            489..1400
                     /locus_tag="PLB01_15345"
     CDS             489..1400
                     /locus_tag="PLB01_15345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HQQ78728.1"
                     /translation="MRRGLVVLEPGILSTVQDLGRPGWGAWGVPASGALDEEALRLAN
                     RLVGNPEDAAALEITLSGPVFKAVGDVLLAVVGGPFGPAPGEVMPIHDGGTLALTAGP
                     GAVRAVVAVAGGIDVPQVLGSRSTCVSARFGGFQGRRLQKGDVLAIGEPFGRPLPGRA
                     NVPRPPAGDVTLRAMPGPQEDLFDPEARAAFWASTWRVLPESNRMGIRLRGASLGFVE
                     TAALPSEGTAPGAVQITAEGQPIVLLAERPTTGGYPKIATVASVDLGLLARTAPGRNV
                     RFERISVDEARRLLAERETSGRAGSAR"
     gene            1397..2224
                     /gene="pxpA"
                     /locus_tag="PLB01_15350"
     CDS             1397..2224
                     /gene="pxpA"
                     /locus_tag="PLB01_15350"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HQQ78729.1"
                     /translation="MTLHAVDYRKKPRFAVDLSFDGGEGFDDEPLMEYVASVNIACGG
                     HAGDERSMRTATRLALRRFVAINAHPSYQDSAGFGRASVDVSPEELELSLTSQIETLR
                     NIVRQEEGELVGVKPHGALYHDAARRADVAACVARAAYAVSPRLVLVTSPGSKLLEEG
                     AALEMVVAVEGFADRAYRPDGSLVPRDEAGAVVTDPKAAVVQALSIVRERRVKTTDGG
                     FHDLTVDTLCLHGDTPGARDIGRAVRAALLKAEVVVRPLRGWKPRGPDPGPPKMPSR"
     gene            2237..4453
                     /gene="purL"
                     /locus_tag="PLB01_15355"
     CDS             2237..4453
                     /gene="purL"
                     /locus_tag="PLB01_15355"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HQQ78730.1"
                     /translation="MGNAAAPLTPAVVAEHGLSPDEFERIKAILGREPSLLELGIYSA
                     LWSEHCSYKSSRPFLKEFPTTGPRVLQGPGENAGAVDIGDGLAVVFKMESHNHPSFIE
                     PYQGAATGVGGILRDVFTMGARPVAILDPLFFGDPEAPRMRDLVDGVVRGIGGYGNCI
                     GVPTVGGMTFFHPAYNKNILVNAMAVGLVKQDRIFRAKASGPGNPVLYAGSKTGRDGI
                     HGASMASDVFDDEKAQRRPTVQVGDPFVEKLVLEAVLEVLEGDAVVAIQDMGAAGLTS
                     STFEMCSRGGVGMRLDLSKVPMRETGMTPYELMLSESQERMVLVVRKGREAEVAAVFR
                     KWGVDVETIGVVQSERKMTLLWNGEVAADLAIGPLVEEAPVYQRPFALPKPLPAATPV
                     SLDGAPAPEAALLRLLASPNLCSKRWIWEQYDVSVRTSTVAGPGGDAAVLRVPGTAKG
                     IAATTDVAPRYVAADPETGAAHAVAEAALNLACVGARPLAVTDCLNFGNPERPEILGQ
                     FVASIRGLAKACRAFETPVVSGNVSLYNETDGVSILPTPTVGMVGLLEDVSTALGARF
                     VREKDLVALLGTTRDEMGAGEYLATVLGRDEGPCPSLDLASVRAVVNLLVEVAGDGMI
                     SSAHDLSSGGLAVALAEASAGGLGAVVNVGMPLSSTRALFAESAGRALVSFPPGRERN
                     LVDAARRHGVAVSLLGRVVPGRLTVTVNGEPAIDLPVSRLATASEEAFVKLVEVRS"
     gene            4450..5028
                     /locus_tag="PLB01_15360"
     CDS             4450..5028
                     /locus_tag="PLB01_15360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQQ78731.1"
                     /translation="MNKLLAVSSVLFFAAAAPAADLKIGAPAPDFTLPSASDGKMVAL
                     KDLLAKNKAVAVIFVATKCPVSNAYNGRMAALGRDYAARGIAVVGINSNKTEPAAEVA
                     AHAKEHGFAFPVVKDDGNKVADAYRAQKTPEVFVISPKGDLLYHGRIDESQDDPRGVK
                     SPDLRNALEAILAGKPVPAAETKAFGCTIKRV"
     gene            5025..5537
                     /locus_tag="PLB01_15365"
     CDS             5025..5537
                     /locus_tag="PLB01_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQQ78732.1"
                     /translation="MRRSARLRAALAAAALACAARTASAEPVKLVKPEQYKARIVAPK
                     KGRVLLVNFWATWCDPCREEMPALVAAAKKFPAKDLAVVLVSVDSLKATPAVEKYLAK
                     EKVPFVCWQVKSHDPQTFVDAVDRDGWNGAVPYTLVYSRSGALVAKLAGPQSEQAFGD
                     AVKKALAGGT"
     gene            5534..6142
                     /locus_tag="PLB01_15370"
     CDS             5534..6142
                     /locus_tag="PLB01_15370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HQQ78733.1"
                     /translation="MRASPAVRARRRPRWDALFIDRDGTLIVERGYLKSPRGVRLSRG
                     AARRLRTFTDEGTLLFVVTNQSGLARGVLTRDEVDAVNAEVVRRLAARGVPVAGVLVC
                     PHFPEGVVPELSIRCRCRKPGTLLHARALAAHGLSARRSAVVGDKWDDVGAGVALGAA
                     QAHVLTGHGREHRAKVVERAPDAILAASLAGALDQLHRRKPP"
     gene            6139..>6395
                     /locus_tag="PLB01_15375"
     CDS             6139..>6395
                     /locus_tag="PLB01_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HQQ78734.1"
                     /translation="MTPREIAVLAAASPSVTVAVAGDLMLDETWIGSVERVAPEAPVP
                     LLALASMSRRAGGAGNVVENLASLGARAVTLGAVGPGEEGA"
BASE COUNT          825 a         2365 c         2297 g          908 t
ORIGIN      
        1 ctcgacgccg tgccgtcggc gcgcacgtgc ctcgtcctcg gcgggccggg gttcgacgcc
       61 tcgccgctcg cggcgctgga agccgatccg cccgcggcgc ctcccagtcc cgctctgcgc
      121 gcgcacgaga tccggttcac gccggacggc gccgacctcg acgagatcgc ctcgcgctgc
      181 ggcctgacgc cggcggagtt cctccgcgcc ttcacggcgc tcacgtacac ggtgggcttc
      241 ctcgggttca cgccgggctt cgcgtacctc tacggcctgc cgctcgcttt tcaccttccg
      301 cggcgccccg gcccgcgcct cgcggtgccc gcgggcagca tcgcgctcgc gggcccgtac
      361 gtcgggatct acccgggcgc gacgccgggc ggctggaacc tcctcgggac gacggcgcag
      421 cgcctcttcg acctccgcgg cgacccgccg tttctcttct ccccgggcga cacggtgagg
      481 ttcatcgcgt gaggcgcggc ctcgtcgtcc tcgagccggg gatcctctcg accgtgcagg
      541 atctggggcg gccgggctgg ggcgcgtggg gcgtgccggc gtcgggcgct ctcgacgagg
      601 aagccctgcg tctcgcgaac cgtctcgtcg gcaacccgga ggacgccgcg gcgctcgaga
      661 tcacgctctc gggcccggtc ttcaaggcgg tgggcgacgt cctcctcgcg gtcgtcggcg
      721 gcccgttcgg gcccgcgccc ggcgaggtga tgccgatcca cgacggaggg acgctcgcgc
      781 tgacggccgg gcccggcgcc gtgcgcgcgg tcgtcgccgt ggcgggcggg atcgacgtcc
      841 cgcaggtcct cggcagccgc tcgacgtgcg tgtcggcgcg cttcggcggg ttccagggcc
      901 ggcggctcca gaagggcgac gtcctcgcga tcggcgagcc gttcgggagg ccgcttccgg
      961 gccgtgcgaa cgtcccgcgg cctccggcgg gcgacgtgac ccttcgggcg atgccgggtc
     1021 cccaggaaga tctgttcgac cccgaggcgc gcgccgcgtt ctgggcgtcc acgtggcgcg
     1081 tcctcccgga gtcgaaccgg atgggaatcc ggctgcgcgg cgcgtcgctc ggattcgtcg
     1141 agacggcggc gctcccttcc gagggaaccg ccccgggcgc ggtccagatc accgcggaag
     1201 ggcagccgat cgtcctcctc gccgagcggc ccacgacggg cggctacccg aagatcgcga
     1261 cggtggcctc ggtcgatctc ggcctcctcg cgaggacggc gccgggccga aacgtgcgct
     1321 ttgagagaat ctccgtcgac gaagcccgga gactcctcgc ggaacgcgag acgagcggcc
     1381 gggccgggag cgctcgatga ccctgcacgc cgtcgactac cgcaagaagc cgcgtttcgc
     1441 cgtcgacctg agtttcgacg gcggagaggg tttcgacgac gagccgctca tggaatacgt
     1501 cgcgagcgtg aacatcgcct gcggcggcca cgcgggcgac gagcgctcga tgcggacggc
     1561 cacgcgcctc gcgctccggc gcttcgtcgc catcaacgcg catccgtcgt accaggattc
     1621 ggccggcttc ggccgcgcgt cggtcgacgt ctctcccgag gagctcgagc tgtccctgac
     1681 gagccagatc gagaccctgc gcaacatcgt tcggcaggag gagggcgagc tcgtgggcgt
     1741 caagccgcac ggggcgctct accacgacgc ggcgcgccgc gcggacgtcg cggcgtgcgt
     1801 cgcccgggcc gcgtacgcgg tgtccccgcg cctcgtcctc gtcacgtcgc ccggctcgaa
     1861 gctcctcgag gagggcgccg ccctcgagat ggtcgtcgcg gtcgagggct tcgcggaccg
     1921 tgcgtaccgg cccgacggct ccctcgtccc gcgcgacgag gccggcgccg tcgtgacgga
     1981 cccgaaggcg gcggtcgttc aggccctctc gatcgtccgc gagcggcgcg tgaagacgac
     2041 ggacgggggc tttcacgacc tcaccgtcga cacgctctgc ctccacggcg acacgcccgg
     2101 ggcccgcgac atcggccgcg cggtgcgcgc cgcgctcctc aaggcggagg tcgtcgttcg
     2161 ccccctccgg ggctggaagc cccgggggcc ggaccccggc ccgccgaaga tgccgagccg
     2221 gtaaactacg gcctcgatgg ggaacgcggc cgcgcctctc acccccgccg tcgtggcgga
     2281 gcacggcctc tcgccggacg agttcgagcg catcaaggcg atcctcggcc gcgagcccag
     2341 cctcctcgag ctcgggatct attcggccct gtggagcgag cactgctctt acaagtcgtc
     2401 gcgacccttc ctgaaggagt tcccgacgac gggcccgcgc gtcctccagg gcccgggcga
     2461 gaacgcgggg gccgtggaca tcggcgacgg cctcgccgtc gtgttcaaga tggagtccca
     2521 caaccacccg tcgttcatcg agccgtacca gggcgccgcg accggcgtgg gcgggatcct
     2581 ccgcgacgtc ttcacgatgg gcgcgcggcc cgtggcgatc ctcgacccgc tcttcttcgg
     2641 agacccggag gcgccgcgga tgcgcgatct cgtggacggc gtcgtgcgcg gaatcggcgg
     2701 ctacggaaac tgcatcggcg tgccgaccgt cggcgggatg acgttcttcc accccgccta
     2761 caacaagaac atcctcgtga acgcgatggc cgtcggcctc gtcaagcagg accgcatctt
     2821 ccgggcgaag gcctccggcc ccggcaaccc cgttctctac gcgggctcga agacgggccg
     2881 cgacgggatc cacggggcgt ccatggcctc ggacgtgttc gacgacgaga aggcgcagcg
     2941 gcggcccacg gttcaggtgg gcgatccctt cgtcgagaag ctcgtgctcg aggccgtcct
     3001 cgaggtcctc gagggcgacg cggtcgtcgc gatccaggac atgggcgccg cgggcctcac
     3061 gtcctccacg ttcgagatgt gctcgcgcgg cggcgtcggg atgcgcctcg acctctcgaa
     3121 ggtccccatg cgcgagacgg ggatgacgcc ctacgagctc atgctgtccg aatcgcagga
     3181 acggatggtc ctcgtcgtgc gcaaggggcg cgaggccgag gtcgcggccg ttttccgcaa
     3241 gtggggcgtg gacgtcgaga cgatcggcgt cgtgcagtcc gagcggaaga tgacgctcct
     3301 gtggaacggc gaggtcgcgg cggacctcgc gatcggcccg ctcgtcgagg aggcgccggt
     3361 ctaccagcgc ccgttcgcgc tgcccaaacc gctgcccgcg gcgacgccgg tctccctcga
     3421 cggcgcgccc gcgccggaag ccgcgcttct ccggcttctc gcgtcgccga acctctgcag
     3481 caagcgctgg atctgggagc agtacgacgt gagcgtccgc acgagcacgg tcgcagggcc
     3541 cggcggcgac gccgccgtcc tgcgcgtgcc cgggacggcg aagggaatcg cggcgacgac
     3601 ggacgtcgcc ccgcgctacg tcgcggcgga ccccgagacc ggcgcggccc acgcggtcgc
     3661 cgaggcggcg ctgaacctcg cgtgcgtcgg cgcgcgcccg ctcgcggtca cggactgcct
     3721 gaacttcggc aaccccgagc ggcccgagat cctggggcag ttcgtcgcgt ccatccgcgg
     3781 cctcgcgaag gcctgtcgcg cgttcgagac gcccgtcgtc tcgggcaacg tgtcgctcta
     3841 caacgagacg gacggcgtct cgattcttcc gactccgacg gtcgggatgg tcggcctcct
     3901 cgaggacgtc tcgaccgccc tcggcgcgcg cttcgtccgc gaaaaggacc tcgtggccct
     3961 cctcgggacg acccgggacg agatgggcgc gggcgaatac ctcgccacgg tcctcgggcg
     4021 cgacgagggc ccgtgcccgt cgctcgacct cgcgtcggtc cgggcggtcg tgaacctcct
     4081 cgtcgaggtc gccggcgacg gcatgatctc ctcggcccac gacctctcgt cgggcggtct
     4141 cgccgtcgcg ctcgccgagg cttcggccgg cggcctcggc gcggtcgtca acgtcggcat
     4201 gcccctgtct tccacgcgcg cgctcttcgc ggaatccgcc gggcgcgcgc tcgtctcgtt
     4261 cccgcccggg cgcgagcgca acctggtgga cgcggcgcgc cggcacggcg tcgccgtctc
     4321 actcctcggc cgcgtcgtcc ccggacgtct caccgtcacc gtcaacggcg agcccgcgat
     4381 cgacctgccc gtgtcccgtc tcgccacggc ctcggaagag gccttcgtca agctcgtgga
     4441 ggtccgttcg tgaacaagct cctcgccgtc tcctccgtcc tcttcttcgc cgccgccgcg
     4501 cccgccgcgg acctgaagat cggcgccccc gcgcccgact tcacgctccc gtccgcgtcg
     4561 gacgggaaga tggtggcgct gaaggacctg ctcgcgaaga acaaggccgt cgcggtcatc
     4621 ttcgtcgcga cgaagtgccc ggtctcgaat gcctacaacg gccgcatggc cgcgctcggg
     4681 agggactacg ccgcgagggg gatcgccgtc gtcggaatca actccaacaa gacggagccc
     4741 gccgcggaag tggccgcgca cgcgaaggag cacgggttcg ccttcccggt cgtgaaggac
     4801 gacggaaaca aggtcgcgga cgcctaccgc gcgcagaaga cccccgaggt gttcgtgatc
     4861 tcgccgaagg gcgacctcct gtaccacggg cgtatcgacg agtcgcagga cgatccgagg
     4921 ggcgtgaagt cccccgacct gcgcaacgcg ctcgaggcga tcctcgcggg caagcccgtg
     4981 cccgcggccg agacgaaggc gttcgggtgc acgatcaagc gggtgtgagg cgctccgcgc
     5041 gcctccgggc ggccctggcg gcggcggcgc tggcctgcgc ggcgcgaacg gcttcggccg
     5101 agcccgtcaa gctcgtcaag cccgagcagt acaaggcgcg catcgtcgcg ccgaagaaag
     5161 ggcgcgtcct gctcgtgaac ttctgggcga cgtggtgcga cccgtgccgc gaggagatgc
     5221 ctgcactcgt cgccgcggcg aagaagttcc ccgcgaagga cctcgccgtc gtcctcgttt
     5281 cggtcgactc cctcaaggcg acgccggccg tcgagaagta cctcgcgaag gaaaaggttc
     5341 cgttcgtctg ctggcaggtc aagagccacg atccgcagac cttcgtcgac gcggtggacc
     5401 gggacggctg gaacggagcg gtcccgtaca cgctcgtcta ctcccgctcg ggagcgctcg
     5461 tcgcgaagct cgccggtccg cagtcggaac aggcgttcgg cgacgcggtc aagaaggcgc
     5521 tcgccggggg cacttgaggg cctcgccggc cgtgcgggcc cggcggcgcc cgcgctggga
     5581 cgcgctcttc atcgaccgcg acggaacgct catcgtcgag cgcggctacc tgaagagccc
     5641 gcgcggcgtc cgtctctcgc gcggtgcggc ccggcgcctc aggacgttca cggacgaagg
     5701 cacgctcctc ttcgtcgtca cgaaccagtc gggactcgcc cgcggcgtcc tgacgcggga
     5761 cgaagtcgac gccgtgaacg cggaggtcgt ccggcggctc gcggcccgcg gcgttcccgt
     5821 ggccggcgtc ctcgtctgcc cgcactttcc cgaaggcgtg gtgccggagc tttcgatccg
     5881 ctgccggtgt cgcaagcccg gcacgctcct ccacgcgcgg gcgctcgccg cgcacgggct
     5941 ttcggcgcgg cgctcggccg tcgtcggcga caaatgggac gacgtcggag ccggcgtcgc
     6001 gctcggggcg gcgcaggccc acgtcctcac ggggcacggc cgggagcacc gcgcgaaggt
     6061 cgtggagcgc gcgccggatg cgatactggc ggcctcgctg gccggtgcgc tcgaccagct
     6121 tcaccggagg aaaccgccgt gacaccccgg gaaatcgccg ttctggccgc ggcctcgccc
     6181 agcgtcacgg tcgccgtggc gggcgacctc atgctcgacg agacgtggat cggcagcgtc
     6241 gagcgcgtcg ccccggaagc gcccgttccg ctcctggccc tcgcgtccat gagccggcgc
     6301 gcgggcgggg cgggaaacgt ggtcgagaac ctcgcgtcgc tcggcgcgcg cgccgtcacg
     6361 ctcggcgccg tcggcccggg cgaggagggc gcgtg
//
LOCUS       DAOMFY010000233         6380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4029_length_6380_cov_8.325375, whole genome shotgun sequence.
ACCESSION   DAOMFY010000233 DAOMFY010000000
VERSION     DAOMFY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4029_length_6380_cov_8.325375"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /gene="bshC"
                     /locus_tag="PLB01_15380"
     CDS             <1..1422
                     /gene="bshC"
                     /locus_tag="PLB01_15380"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HQQ78735.1"
                     /translation="SRGAEGLPGGGGGAARAARGERAAVLTGQQAGLFGGPHLTLVKA
                     VAAEKIASDLSASGAPASAAFWCAAEDHDLVEVTRVVLPTPEGPRDFGPDPAALGANR
                     APVGALPIAADLDGILGAAAANAGGLPDEDALAALRTAHAGATYGAAFTRTLGWLLDG
                     ELPVVDAADPAEKPALVPLAVRLVRERRDVRARLEARGAALAKAGHPLQVKTDPAALP
                     LFVRVGDERLLLVEETGRLALKSRDGTFAEEDVVARIESGDWLPSFSALTRPLAASVL
                     YPVGATVLGPAEVAYWAQAWPLFEWAGLVPPAVLLRPFVAFETPSARRLLAKLDVSLA
                     DVLEGDDALLRRKGAGSARSLLARVAAIRARAAAELDAARPALVAVDPSLEKAVAVTR
                     EKLAYAFEKLVEKTEAAAGRADAQVAQQVRRLAGELLPDGKLAERLYPVLPYVLKLGR
                     GAVVGALRRDLKWDEPGLQEIVL"
     gene            1419..2171
                     /gene="bshB1"
                     /locus_tag="PLB01_15385"
     CDS             1419..2171
                     /gene="bshB1"
                     /locus_tag="PLB01_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HQQ78736.1"
                     /translation="MNLDDAVPVDVLAFGPHPDDVELGCGGTLAALAGRGRGVGIVDL
                     TRGEMATRGTPETRAAEAAEAARILGARFRVNLDLGDGDLRTDRAAQLRVVEAVRRAR
                     PRLVLAPWTEDRHPDHERAGRLVGEAAWYAGLAKLETGLPPHRPDQVVFYAAYALLSP
                     TFLVDVTATFETKRAALRAYRSQFHDPSAPATGAPETYVSSKSFWDGVEARARSYGRV
                     ANVAFAEGFVSKAPPTLADPAAAFAGYEGAKP"
     gene            2168..3289
                     /gene="bshA"
                     /locus_tag="PLB01_15390"
     CDS             2168..3289
                     /gene="bshA"
                     /locus_tag="PLB01_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HQQ78737.1"
                     /translation="MKIGITCYPTFGGSGVVATELGHELARRGHDVHFITYAMPSRLN
                     VFADRVTYHEVTVPSYPLFQYAPYDLALATRMGDVATHEKLDLIHVHYALPHAISAHL
                     AREMLAPVRLGLVTTLHGTDVTIVGQDRSYLPITRFGIEKSDAVTAVSEHLKQVTIDV
                     FQPKKDIVVIPNFIDPVRFSPESPQLSCRFVPAGRRVLMHVSNFRPVKRVLDVVEVFD
                     RVQKRVPASLVMVGDGPDRPAAEALCREKGLAGHVTFLGNMPAVEALMPSADLYLLPT
                     DSESFGLSALEAQACGVPVLGYAVGGLPEVVVSGETGFLRAVGDVAGLADDGAALLLD
                     DARYKAISQTARARAVKLFNADAIVSRYLALYERVLAAA"
     gene            complement(3250..4245)
                     /locus_tag="PLB01_15395"
     CDS             complement(3250..4245)
                     /locus_tag="PLB01_15395"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HQQ78738.1"
                     /translation="MGIAPSAARFLRRLAAAPAVELHLHLEGAVSAAALVRLSSRAPE
                     PIFPDLASVRARRRALGSPEAFFSFYRDVCRQIRSAADYGAVARALVTRLAKERIRHA
                     EVYVSPAVAEKLGLDWFDVREALEAVFAAHEAAGRGRIVVLLDAVRHWGAESASRVLD
                     LHERSPWPRAAGFGLGGEETAFPARDFADAFRRARRLGLMPVAHAGEWAGPDSVAETL
                     THLSPVRLAHGIRAAEDPALLALLARRSIVCDVCPTSNLATGAVPRGATHPVRALLAA
                     GVPVTLSTDDPGLFGTTLRREFRRVAGWGATGGELLACARTARHAAARTLSYSAR"
     gene            4318..4701
                     /locus_tag="PLB01_15400"
     CDS             4318..4701
                     /locus_tag="PLB01_15400"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQQ78739.1"
                     /translation="MRAAAFGVDLLLVAGGPLLVASAIVFGIAVVSEEAPVGLDEGFR
                     AAQVLAGLLFLFRDAAGGSPGKKLFGLRLVQKGGSGSGPAASLARNLTLLVPGWNLIE
                     IASVIRRRDGRRPGDKLAGTTLVEA"
     gene            4759..5589
                     /locus_tag="PLB01_15405"
     CDS             4759..5589
                     /locus_tag="PLB01_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HQQ78740.1"
                     /translation="MYAKPRTRPYRLARIGGAFAAALLAVAASSGARADDAKEERFEN
                     TYELTGVRKVRVQNVNGAVNIESGGEHLKVVAVKSVRRASDADVLKETEIRVTKTGSA
                     IEIETILPKQGRFFRWSFFGRSSAADVAYQVTLPAGVAVEAETVNGRIGASKRTGDLV
                     LSTVNGAVKVDGQDGPLKVNTVNGSVEVSFANAMRQASLETVNGSVTVSCSKDSSIRC
                     ALQTTNGRIQSEFPVTIEGKWGPKEAHGSINGGKESLAVETVNGDVRLFVADSSAVRK
                     "
     gene            5651..6298
                     /locus_tag="PLB01_15410"
     CDS             5651..6298
                     /locus_tag="PLB01_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78741.1"
                     /translation="MSRLQRITLAAAALVALPALGQFKTPVPIVSPTPVPGVPPRELP
                     NHGPKELPPTRTPAPTRTPTRTATPTPMPPDSRYAGPTWGPDRYALPIAVTRLDSNSQ
                     CKSNAFFFNSGMFLVSIDCYAQGRGPLGLKVDAEVAKGLRLKNGWKVKSVTIGSWDGT
                     EPPNPDSHHGFRVTKSPVVGSDDPSVSLHLFSDGFKGIFVRGGVLIEGPHGTTPW"
BASE COUNT          881 a         2316 c         2302 g          881 t
ORIGIN      
        1 agccgcgggg ccgagggcct gccgggaggg ggtggtggtg ccgcgcgggc tgcgcggggc
       61 gagcgggccg ccgtcctcac cggccagcag gccggtctct tcggcggccc gcacctcacg
      121 ctcgtgaagg ccgtcgccgc ggagaagatc gcgtcggacc tgtcggcctc gggcgcgccc
      181 gcttccgccg cgttctggtg cgcggccgaa gatcacgacc tggtcgaggt cacgcgtgtc
      241 gtcctgccga cccccgaggg accgcgtgac ttcgggcccg atccggccgc gcttggggcg
      301 aaccgggcgc ctgtcggcgc gcttccgatc gccgcagacc tcgacggaat cctcggagcg
      361 gcggccgcga acgccggcgg cctgcccgac gaggacgccc ttgccgcgct caggaccgcg
      421 cacgcgggcg cgacgtacgg cgcggccttc acgcggacgc tcggctggct gctcgacggg
      481 gagctcccgg tcgtggatgc ggccgacccg gccgaaaagc ccgcgctcgt gccgctcgcc
      541 gtccgcctcg tgcgggagcg gcgcgacgta cgtgcgcgcc tcgaggcgcg cggagcggcg
      601 ctcgcgaagg ccggccatcc gctccaggtg aagacggatc ccgccgcgct gcccctcttc
      661 gtgagagtgg gggacgaaag gctgcttctc gtcgaagaaa cgggccggct cgcattgaag
      721 agccgagacg gaacgttcgc cgaggaggac gtcgtcgcgc ggatcgagtc cggggactgg
      781 ctgccgtcgt tctcggcgct cacgcgcccg ctcgcggcct cggtcctcta ccccgtcggc
      841 gcgacggtgc tcggtccggc ggaggtggcc tactgggcgc aggcgtggcc cctcttcgaa
      901 tgggcgggtc tcgtccctcc cgccgtcctc ctccgcccct tcgtcgcctt cgaaacgccc
      961 tcggcgcgaa ggctcctcgc gaagctcgac gtctctctcg cggacgtcct cgagggcgac
     1021 gacgctctcc tccggcggaa gggcgcggga tccgcgcgct ccctcctcgc gcgcgtcgcg
     1081 gcgatccgcg cgcgcgcggc cgcggaactc gacgcggccc ggcccgccct cgtcgccgtg
     1141 gacccttccc tcgaaaaggc cgtcgccgtg acgcgcgaga agctcgcgta cgccttcgag
     1201 aagctcgtcg aaaagacgga ggccgcggcg ggccgcgcgg acgcccaggt ggcccagcag
     1261 gtgcgccggc tcgcgggcga gctcctgccg gacgggaagc tcgccgaacg gctctacccg
     1321 gtcctgccgt acgtactgaa gctcggacgg ggcgccgtcg tcggcgcgct ccgacgggac
     1381 ctgaaatggg acgagcccgg cctgcaggag atcgtgctgt gaacctcgac gacgccgttc
     1441 ccgtggacgt cctcgcgttc ggcccgcacc cggacgacgt cgagctcggg tgcggcggga
     1501 cgctcgcggc cctcgccggc cgcggacgcg gcgtcgggat cgtggacctc acgcgcggtg
     1561 agatggcgac gcgcgggacc cccgagacgc gcgcggccga ggccgccgag gccgcccgaa
     1621 tcctcggcgc acgcttccgc gtgaacctcg acctcgggga cggcgatctc cggacggacc
     1681 gcgccgcgca gctccgcgtc gtcgaggccg tccgccgcgc ccgcccccgc ctcgtcctcg
     1741 ctccgtggac ggaggatcgc catcccgacc acgagcgcgc gggccggctc gtcggcgagg
     1801 ccgcgtggta cgcgggcctc gcgaagctcg agacgggcct gccgccccat cgcccggacc
     1861 aggtcgtctt ctacgcggcg tacgcgctgc tctcgccgac gttcctcgtc gacgtgacgg
     1921 cgacgttcga gacgaagcgc gccgcgcttc gcgcgtaccg gagccagttc cacgatcctt
     1981 ccgcgccggc gaccggcgcg ccggagacgt acgtctcgtc gaaatccttc tgggacggcg
     2041 tcgaggcgcg cgcgcgctcc tacgggcgcg tcgcgaacgt cgcgttcgcg gagggcttcg
     2101 tctcgaaggc cccgccgacg ctcgccgacc ccgccgccgc gttcgcgggc tacgaagggg
     2161 cgaagccgtg aagatcggga tcacgtgcta cccgacgttc ggcgggtcgg gggtcgtcgc
     2221 gaccgagctc gggcacgagc tcgcgcggcg cggccacgac gtccacttca tcacgtacgc
     2281 gatgccctcg cgcttgaacg tcttcgcgga ccgcgtgacg taccacgagg tgaccgtccc
     2341 gtcgtacccg ctcttccagt acgcgccgta cgacctcgcc ctcgcgacgc gcatgggcga
     2401 cgtcgcgacg cacgagaaac tggacctcat ccacgtccac tacgcgctcc cgcacgcgat
     2461 ctcggcgcac ctcgcgcgcg agatgctggc ccccgtgcgc ctcggcctcg tcacgacgct
     2521 ccacgggacg gacgtgacga tcgtcgggca ggaccgctcg tacctcccga tcacgcggtt
     2581 cggaatcgag aagtcggacg ccgtgaccgc ggtgtccgag cacctgaagc aggtcacgat
     2641 cgacgtcttc cagccgaaga aggacatcgt cgtcatcccg aacttcatcg accccgtgcg
     2701 cttctcgccg gagtcgccgc agctgtcgtg ccgtttcgtg cccgcgggcc ggcgcgtcct
     2761 catgcacgtc tcgaacttcc ggccggtcaa gcgcgtcctc gacgtggtcg aagtcttcga
     2821 ccgcgtccag aagcgcgtcc cggcgtcgct cgtcatggtc ggcgacggtc ccgaccggcc
     2881 cgcggccgaa gcgctctgcc gcgagaaggg gctcgcgggc cacgtgacgt tcctcgggaa
     2941 catgcccgcc gtcgaggccc tgatgccgtc ggccgacctc taccttctcc cgaccgactc
     3001 cgaatccttc ggcctctcgg cgctcgaggc gcaggcctgc ggtgtgcccg tgctcggcta
     3061 cgccgtcgga ggcctgcccg aggtcgtcgt ttccggcgaa acggggtttc tccgcgccgt
     3121 cggcgacgtc gccggcctcg cggacgacgg ggctgcactg cttctcgacg acgcgcgcta
     3181 caaggcgatc tcgcagaccg cccgcgcccg cgcggtcaag ctcttcaacg cggacgcgat
     3241 cgtctcgcgc tacctcgcgc tgtacgagag ggtcctggcc gcggcgtgac gggccgtgcg
     3301 cgcgcaggcg agaagctctc cgcccgtcgc gccccagccg gcgacgcgcc ggaattccct
     3361 ccgcaacgtc gtcccgaaga ggccggggtc gtcggtcgac agcgtgacgg gcacgccggc
     3421 cgcgaggagg gcgcgcacgg ggtgcgtcgc gccccgcggc acggcgccgg tcgcgagatt
     3481 cgacgtcggg cagacgtcgc agacgatgct gcgccgcgcg agcagggcga ggagcgcggg
     3541 atcttcggcg gcgcggatcc cgtgcgcgag gcgaacggga gaaaggtgcg tgagggtctc
     3601 ggcgaccgag tcgggccccg cccactcgcc cgcgtgagcg accggcatca ggccgaggcg
     3661 gcgggcgcgg cggaaggcgt ccgcgaagtc gcgggccggg aaggccgttt cctcgccgcc
     3721 gaggccgaat ccggcggcgc gcggccacgg gctcctttcg tggaggtcga gaacccggct
     3781 cgccgactcg gcgccccagt ggcgcacggc gtcgaggaga acgacgattc gtccccgccc
     3841 cgcggcctcg tgcgcggcga agaccgcttc gagcgcctcg cgcacgtcga accagtcgag
     3901 gccgagcttc tcggcgacgg ccggcgagac gtagacctcg gcgtggcgga tgcgctcctt
     3961 cgcgaggcgc gtgacgaggg cgcgcgccac ggccccgtag tcggcggccg aacggatctg
     4021 ccggcagacg tcccggtaga aggagaagaa cgcctccggc gagccgagcg cccgccggcg
     4081 cgcgcgcacg gacgcgagat ccgggaaaat gggctcgggc gctcgggagg agaggcgcac
     4141 gagcgccgcc gcggaaaccg cgccctcgag gtgaaggtgg agctcgacgg cgggagcggc
     4201 ggcgagacgc cggaggaagc gggcggcgga cggagcgatt cccacgcgga cgcatcctac
     4261 ggcgtcttcc agacggggcc tgtcccgctc ggcgcgccgc cgcgggggcg gctctggatg
     4321 cgcgcggcgg ccttcggcgt cgacctcctg ctcgtggcgg gcgggcccct tctcgtcgcg
     4381 tccgcgatcg tcttcggaat cgcggtcgtg tcggaagagg ccccggtggg tctggacgag
     4441 ggttttcggg cggcgcaggt gctcgcgggc ctcttgttcc tcttcaggga cgccgcggga
     4501 ggaagccccg gaaagaagct cttcggcctg cggctcgtcc agaagggcgg ctcggggtca
     4561 ggcccggcgg cctcccttgc ccggaacctg acgcttctcg tcccgggctg gaatctcatt
     4621 gaaatcgcat cggttattcg tcgccgcgac gggcggaggc ctggcgacaa actggccgga
     4681 acgacgctcg tggaagcctg agagcccttc cccgaaacct ttccccggcg ccgcacgttc
     4741 tctcgggtgg gagccgtcat gtacgcaaag cccagaaccc gcccgtaccg tctcgcccgc
     4801 atcggcggag cgttcgcagc cgcgctgctc gcggtggccg cctcctccgg cgcgcgggcg
     4861 gacgacgcca aggaagagcg cttcgagaac acgtacgagc tgaccggcgt ccggaaggtg
     4921 cgcgtccaga acgtcaacgg cgccgtgaac atcgagtcgg gcggcgagca cctgaaggtc
     4981 gtggccgtga agtcggttcg ccgcgcctcc gacgccgacg tgctgaagga gaccgagatc
     5041 cgcgtgacga agacgggttc ggcgatcgag atcgagacga tcctcccgaa gcagggccgg
     5101 ttcttcagat ggtccttctt cgggcggtcg agcgcggcgg atgtcgcgta tcaggtcacg
     5161 ctgcccgcgg gcgtcgccgt cgaggcggaa accgtcaacg gccggatcgg cgcgtcgaag
     5221 cggacggggg acctcgtcct gtcgacggtg aacggcgccg tgaaggtgga cggccaggac
     5281 ggtcccctga aggtcaacac ggtgaacggc tccgtcgagg tttcgttcgc gaacgcgatg
     5341 cggcaggcca gtctcgagac cgtcaacggc tcggtgaccg tcagctgctc gaaggactcc
     5401 tcgatccgct gcgcgctcca gacgacgaac ggccggatcc agtccgagtt ccccgtcacg
     5461 atcgagggca agtggggccc gaaggaagcc cacggctcga tcaacggcgg caaggagagc
     5521 ctcgccgtcg agaccgtgaa cggcgacgtc cggctgttcg tggccgactc ctcggccgtg
     5581 cgcaagtaac gcgccgcggg ctgaccccgt ccggaggagt cctgcgcgtt ctcccgtgaa
     5641 ggagaacccc atgtcgcgac tccagcggat cacgcttgcg gcggcggccc ttgtcgcgct
     5701 gcccgccctg ggccagttca agacgccggt cccgatcgtc tcgccgacgc cggtgccggg
     5761 cgtcccgccc agggagcttc ccaatcacgg cccgaaggag ctgccgccga cgcgcacccc
     5821 cgcgccgacg cgcaccccga cgcgcaccgc gactccgacg ccgatgccgc cggactcgcg
     5881 ctacgcgggc ccgacgtggg gcccggaccg gtacgcgctt cccatcgccg tcacgcgtct
     5941 cgactccaac tcccagtgca agtcgaacgc gttcttcttc aacagcggca tgttcctcgt
     6001 ctcgatcgat tgctacgcgc aggggcgcgg tccccttggg ctcaaggtgg acgccgaggt
     6061 cgcgaagggg ctgcgcctga agaacggctg gaaggtgaag agcgtcacga tcggctcgtg
     6121 ggacggcacc gagccgccga acccggactc acaccacggg ttccgggtca cgaagagccc
     6181 ggtcgtcggg tcagacgatc cgtcggtctc cctgcacctc ttctcggacg gcttcaaggg
     6241 gatcttcgtg aggggcgggg tcctcatcga gggaccgcac ggcacgacgc cctggtgagc
     6301 gggccgcggg ggagaggata aggaatggtg ccctgggcca gactcgaact ggcacggatt
     6361 gctccacacg cccctcaaac
//
LOCUS       DAOMFY010000234         6332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4082_length_6332_cov_7.918114, whole genome shotgun sequence.
ACCESSION   DAOMFY010000234 DAOMFY010000000
VERSION     DAOMFY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4082_length_6332_cov_7.918114"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLB01_15415"
     CDS             complement(<1..429)
                     /locus_tag="PLB01_15415"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQQ78742.1"
                     /translation="MKFNRRILQSSFSTAEVAKLTGLTARQLDWWDRRGFLRPSVTAA
                     SGYGTRRRYSFTDVVKLRLASRLRAAGFGLPQVRRVVETLKRLDATLDGLASVRLLVA
                     DRRVVWARTDSELVDLLHGGQLMLVFPVGEAVADVARAAED"
     gene            complement(568..1737)
                     /gene="asnA"
                     /locus_tag="PLB01_15420"
     CDS             complement(568..1737)
                     /gene="asnA"
                     /locus_tag="PLB01_15420"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HQQ78743.1"
                     /translation="MKHKTEVPAKKTVALDKKADLAGPGIGNYAELEKVLPNDYMPLL
                     DPKETQRAITAVKRVIEDGLCRELGLMRVEVPLIVDAESGVNDYLDRDGSRGPVTFHI
                     SNDNGKNPIEAQVVQAATKWKRVALAQFGMKPGEGLVTDMRAVRKDYFLDHDHSAYVD
                     QWDWEKVITPEQRSLDFLTATVEGIWRVIKGAEDFALETFPGLKKRKLPTLPDRLVFL
                     HAEDILDMYPDLPRKQRETAILQEHPAVFIYGIGWTLKDGYPHEMRAADYDDWVTQTH
                     AKDGRTMHGLNGDILVWNPITKRRHELSSMGIRVNAETLKKQLALTHQEHFLKFPYHQ
                     GIVNGTLPLSIGGGIGQSRVLMLLLRKAHLGEVSVTVWPKILKEMCAKKNIHVIE"
     gene            1984..2925
                     /locus_tag="PLB01_15425"
     CDS             1984..2925
                     /locus_tag="PLB01_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQQ78744.1"
                     /translation="MRIPRVRRAGAGASASPSSSFKALLFGLLFLASAAAGPPASAAP
                     GDLVRTVRYKISAGDLFTGEAAAAEYKREHGVDAEYLNAVGWLARGAPLLGKTDKAWR
                     YVAEVRREIPEEKDGLTVPLGAAIETEGRLRAARDGRGAALKFLEAEYARAKDTELRC
                     RIRKNVNLLSLEGQPAPEMTATVPKGRPAVLFFWAHWCGDCKAQAPALGRVLEKYRAK
                     GLAMVAPTRLFGTGAENKPATPAEEKAWIEKVWTESYGMLGGVPTPIDTETMVRWGVS
                     SNPTLALVDRKGLVRLYTPTRLSEEELSRRVEELLAE"
     gene            complement(2935..4764)
                     /locus_tag="PLB01_15430"
     CDS             complement(2935..4764)
                     /locus_tag="PLB01_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQQ78745.1"
                     /translation="MGILLTTTGLTKAFGSHPLFEGIGCSVDSGDRIGLIGPNGAGKS
                     TLLAILAGEIPPDEGTRSAQRGLRIGYLSQMPVFREGATIREAVLEGAAGGHAGEEDW
                     EAGFAADEYIARLSLATEGRSAATPVATLSGGWKKRVALARELVRKPDLLLLDEPTNH
                     LDVDAILWLEELLARAPFATLTVTHDRVFLQRVANRILELDRRNANGLLDVRGDYATY
                     LRVKEERLATDAQRELSLRNQLRRETEWLLRGAKARSTKQQARIQRAGALETEVGQLA
                     ERNRSQNVRLDFESSGKNPKRLIEAKGIGKAYGANVLFSQLDLLVTPHSRIGLLGPNG
                     CGKSTLLRILLGTERPDTGEVFRAENLQPVTFEQGRESLDPDSTVARTICPEGDHVEY
                     RGALVHIRSYLDRFLFKEEQRDMKVGRLSGGEQSRLLLARLMLKKTNLLVLDEPTNDL
                     DLATLQVLEDCLADFPGAVILVTHDRYFLDRVTNRILAFGKDEKGEGTLTALAGLDQW
                     EAWFAERKGAAPARRTAAKPSARKRRVGFNEQRELDGIEARVQAAEAARDALRDESEH
                     PDHASDALKLVAILAALEEKQDEVDRLYARWAELERLAAGDPA"
     gene            complement(4778..4854)
                     /locus_tag="PLB01_15435"
     tRNA            complement(4778..4854)
                     /locus_tag="PLB01_15435"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4818..4820),aa:Asp,seq:gtc)
     gene            4911..>6332
                     /locus_tag="PLB01_15440"
     CDS             4911..>6332
                     /locus_tag="PLB01_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78746.1"
                     /translation="MTGCKRASTLLLAVLWAAPAPGAEGMRILTFVPGVVQGRLPVTV
                     DLGSAPPPAELLLDGKSVCRVMSRRQTCTVDLGPAPRVRLLELVRRGSAGPAERVRRW
                     VNKPSVAHAEVLPRTDCVKASGPCTLSVAWSHEQDLDPALLSVSIDGKKEYEGPPKNV
                     PIPFAAERPVRVVAVDLSFIDGQRASETLLVGSGTSSAVEAPLNAVVVSTRGKEGAAR
                     TAPATLGGRVVRAAEPGESDLLFFVAPSAVPKLLALEAGAQKSFSKTNSRLAKLLDGV
                     THVIHFSPLATRASYRTAAEVSDRLFRLQGFDCTPGSGHRQLILPCVDASVLTGGQYR
                     LAATVTSGAFVRAATPRRRVAVVVLGDGESSPDESAFGPAEARAYLSEIFVPLAVWRV
                     GKAEGGAWGEGRRVTTPQEFVDAFAQLRAELDAQKICWVAEDLDPASFRLAAGDEGLV
                     LAGRGAGAAPLAVVSAAEPEEPGT"
BASE COUNT          882 a         2209 c         2243 g          998 t
ORIGIN      
        1 gtcctccgcg gcgcgcgcga cgtcggcgac cgcttcgccg accgggaaga cgagcatgag
       61 ctgcccgccg tgaaggaggt cgacgagctc gctgtccgtc cgcgcccaca cgacgcggcg
      121 gtccgcgacc agcaggcgca cggacgcgag cccgtccagg gttgcgtcga ggcgcttcag
      181 ggtctcgacg acgcgccgca cctggggcag gccgaacccg gccgcgcgca ggcgcgaggc
      241 gaggcgcagc ttcacgacgt ccgtgaacga gtaacgcctg cgcgtgccgt aaccggacgc
      301 cgccgtcacc gacgggcgca ggaacccgcg gcgatcccac cagtccagct ggcgggctgt
      361 gagccccgtc aacttcgcga cctcggcggt cgagaaggaa gactggagga tccggcgatt
      421 gaatttcacg tgtgtatttc aatcataccc gaaaaatgcc gaattggccg cggaaggggc
      481 cagaacatca agagtttctt cgaaagaaaa gagcggccgc gaagccgcgg ccgccccctc
      541 ttcatcggga tctgaacctc cgggtcgtta ctcgatgacg tggatgttct tcttcgcgca
      601 catctccttg aggatcttcg gccacacggt cacgctgacc tccccgaggt gcgccttgcg
      661 caggaggagc atgagcacgc gcgactggcc gatgccgccg ccgatcgaga gcggcagcgt
      721 cccgttcacg atgccctggt ggtacgggaa cttcaggaaa tgctcctggt gcgtgagcgc
      781 gagctgtttc ttcagcgtct cggcgttcac gcggatgccc atcgacgaga gctcgtggcg
      841 gcgtttcgtg atcgggttcc agacgaggat gtcgccgttg agcccgtgca tcgtgcggcc
      901 gtccttcgcg tgcgtctggg tgacccagtc gtcgtagtcg gcggcacgca tctcgtgcgg
      961 gtagccgtcc ttcagggtcc agccgatccc gtagatgaag acggccgggt gctcctgcag
     1021 gatcgccgtc tcgcgctgct tgcgcggaag atccgggtac atgtcgagga tgtcctccgc
     1081 gtggaggaac acgagcctgt cgggcagcgt cgggagcttc ctcttcttga ggcccgggaa
     1141 cgtctcgagc gcgaagtcct ccgcgccctt gatgacgcgc cagatcccct cgacggtcgc
     1201 cgtcaggaag tcgaggctcc tctgctcggg cgtgatgacc ttctcccagt cccactggtc
     1261 gacgtacgcg ctgtggtcgt ggtcgaggaa gtagtccttg cgcacggcgc gcatgtcggt
     1321 cacgaggccc tcgccgggct tcatcccgaa ctgcgcgagc gccacgcgtt tccacttcgt
     1381 ggcggcctgg acgacctgcg cctcgatggg gttcttgccg ttgtcgttcg agatgtggaa
     1441 cgtgacgggg ccgcgcgaac cgtcgcggtc gaggtagtcg ttcacaccgc tttcggcgtc
     1501 gacgatgagc ggcacttcga cgcgcatgag gcccagctcg cggcagaggc cgtcctcgat
     1561 gaccctcttg acggccgtga tcgcgcgctg cgtctccttt gggtcgagga ggggcatgta
     1621 gtcgttcggg aggaccttct cgagctcggc gtagttcccg atcccgggac ccgcgaggtc
     1681 ggccttcttg tcgagggcga cggtcttctt cgcggggact tcggtcttgt gcttcatgcg
     1741 cacgtccttt ctccggggag cgccgccgga cgtgcgacac acggatcacg ggcggacggc
     1801 cggaccccga cgcgtccccg aggggacgag ttccagaaag aacatggggc cgcccgcggc
     1861 ggcccgcgac tgatgcggac gcatcagggg ctggcacggt ccctgctcag ccgaagcgcc
     1921 ccacgccgaa aaggcgggaa gaagagagaa gaggacgatc ttcttagaaa gaggtcgcac
     1981 gtgatgcgca ttcctcgtgt ccgccgggcc ggtgccggag cctcggcgtc cccatcctca
     2041 tcgttcaaag ctcttctctt cggtcttctc tttctcgcct cggcggcggc cggcccgccg
     2101 gcctccgccg cccccggcga cctcgtccgg accgtgcgct acaagatctc cgccggcgac
     2161 ctcttcacgg gcgaggcggc cgccgccgag tacaagcgcg agcacggcgt cgacgccgag
     2221 tacctgaacg cggtcggctg gctcgcccgc ggcgcgccgc tcctcggcaa gaccgacaag
     2281 gcctggagat acgtcgccga ggtgcggagg gagatcccgg aggagaagga cggcctcacg
     2341 gttccgctcg gcgcggcgat cgagacggag ggccgcctcc gggcggcgcg tgacgggcgc
     2401 ggagcggcgc tgaaattcct cgaggccgag tacgcgcgcg cgaaggacac cgagctgcgt
     2461 tgccggatcc gcaagaacgt caacctcctc tctctcgaag gccagcccgc gccggagatg
     2521 accgcgacgg tcccgaaggg ccggcctgcc gtcctcttct tctgggccca ctggtgcggc
     2581 gactgcaagg cgcaggcgcc ggccctcgga cgcgtcctcg agaagtaccg cgcgaagggc
     2641 ctcgcgatgg tcgctccgac gcgcctcttc ggcacgggcg cggagaacaa gcccgcgacg
     2701 ccggccgaag agaaagcctg gatcgagaag gtgtggacgg agtcgtacgg gatgctcggc
     2761 ggcgtgccca cgccgatcga cacggagacg atggtccgct ggggcgtgtc gtcgaacccg
     2821 acgcttgcgc tcgtggaccg aaagggcctc gtccgcctct acacaccgac acgcctctcg
     2881 gaagaggagc tctcccggcg ggtggaagag ctcctcgcgg agtagcggac cggtctacgc
     2941 cgggtcgccc gccgcgaggc gctcgagctc ggcccaccgc gcgtagaggc ggtcgacctc
     3001 gtcctgcttt tcctcgagag cggcgaggat cgcgacgagc ttcagcgcgt cgctcgcgtg
     3061 gtccggatgc tcgctctcgt cgcgcagtgc gtcgcgggcg gcttccgccg cctggacgcg
     3121 cgcctcgatc ccgtcgagct ccctctgctc gttgaagccg acccggcgct tccgcgcgga
     3181 gggcttcgcg gcggtgcggc gcgccggggc ggcgcccttc cgctccgcga accacgcttc
     3241 ccactggtcg aggccggcga gcgccgtgag cgtcccttcg cccttctcgt ccttcccgaa
     3301 cgcgaggatg cggttcgtga cgcggtccag gaagtaccgg tcgtgcgtca cgaggatgac
     3361 ggcacccggg aagtccgcga ggcagtcctc gaggacctgg agcgtcgcga ggtcgaggtc
     3421 gttcgtgggc tcgtcgagga cgaggaggtt cgtcttcttc agcatgagcc gcgcgaggag
     3481 cagccggctc tgctcgccgc ccgagaggcg gccgaccttc atgtccctct gctcttcctt
     3541 gaacaggaag cggtcgaggt agctccggat gtggacgagc gcgccgcggt actcgacgtg
     3601 gtcgccctcg gggcagatcg ttctggcgac cgtcgagtcc gggtcgaggc tctcgcggcc
     3661 ctgctcgaac gtgaccggct ggaggttctc cgcgcggaag acctcgcccg tgtcgggccg
     3721 ctccgtgccg agcaggatcc ggagcagcgt cgacttgccg cagccgttcg gcccgaggag
     3781 gccgatccgg ctgtgcggcg tcacgaggag gtcgagttgc gagaagagga cgttcgcgcc
     3841 gtaggccttg ccgatgccct tcgcctcgat gaggcgcttc gggttctttc cggacgactc
     3901 gaagtcgagg cggacgttct gcgagcggtt ccgctccgcg agctgcccga cctcggtctc
     3961 cagcgcgccc gccctctgga tgcgcgcctg ctgcttcgtc gagcgcgcct tcgcgccgcg
     4021 gaggagccac tccgtctcgc gccggagctg gttccggagc gagagctcgc gctgcgcgtc
     4081 ggtcgcgagg cgctcttcct tcacgcggag gtacgtcgcg tagtcgcccc ggacgtcgag
     4141 gaggccgttt gcgttccggc ggtcgagctc gaggatgcgg ttcgcgacgc gctgcaggaa
     4201 cacccggtcg tgcgtcaccg tgagcgtcgc gaacggcgcg cgggcgagga gctcctcgag
     4261 ccagaggatc gcgtcgacgt cgaggtggtt cgtgggctcg tcgagaagga ggaggtcggg
     4321 cttcctgacg agctcgcgcg cgagcgcgac gcgcttcttc cacccgccgg agagcgtcgc
     4381 gacgggcgtc gcggccgagc ggccctcggt cgcgagcgag agccgcgcga tgtactcgtc
     4441 ggccgcgaac ccggcttccc agtcttcctc gccggcgtgg ccgcccgcgg cgccctcgag
     4501 caccgcctcc cggatcgtcg cgccctcgcg gaacacgggc atctgggaca ggtatccgat
     4561 gcgcagcccc ctctgggccg agcgcgtgcc ctcgtccggc gggatctcgc ccgcgaggat
     4621 ggcgagcagc gtggacttgc ccgcgccgtt cgggccgatg aggccgatgc ggtcgcccga
     4681 gtccacggag cacccgatcc cctcgaacag gggatgggag ccgaaggcct tcgtgagacc
     4741 cgtcgtggtg aggaggattc ccatggggag gggagagtgg cggggccgac ggggctcgaa
     4801 cccgcgacct caggcgtgac aggcctgcgc tctaaccaac tgagctacga ccccgatttt
     4861 ttcgtccctt cgcaggtgcg cgacgggagg acgcggagta tacagaggtc gtgacgggct
     4921 gcaagcgcgc ctcgaccctc ctcctggccg tcctctgggc cgctccggcg ccgggcgcag
     4981 agggaatgcg gatcctgacg ttcgtgcccg gcgtcgtaca gggacggctc ccggtcacgg
     5041 tcgacctcgg gtccgcgcca cccccggcgg agctcctcct cgacggcaag agcgtctgcc
     5101 gcgtgatgag ccgccgccag acgtgcaccg tggacctcgg acccgctccc cgcgtgcgcc
     5161 tcctcgagct cgtgcgccgc gggagcgcag gcccggccga gcgcgtgcgc cgctgggtga
     5221 acaagccttc cgtcgcgcac gccgaggtcc tgccccgcac cgactgcgtg aaggcgagcg
     5281 ggccgtgcac gctgtccgtc gcgtggtcgc acgagcagga tctcgacccc gctctcctca
     5341 gcgtctcgat cgacgggaag aaggagtacg aggggccccc gaagaacgtc ccgatcccct
     5401 tcgcggccga gcggccggtg cgggtcgtcg ccgtcgacct ctccttcatc gacggccagc
     5461 gtgcgtccga gacccttctc gtcgggagcg gcacgtcgag cgccgtcgag gctccgctca
     5521 acgccgtcgt cgtgtcgacc cggggcaagg agggcgccgc gcggaccgcg cctgccacgc
     5581 tcggtggccg cgtcgtccgc gccgccgaac ccggcgagag cgacctcctc ttcttcgtcg
     5641 cgccctcggc ggtcccgaag ctcctcgccc tggaggcggg ggcgcagaag tcgttctcca
     5701 agacgaactc gaggctcgca aagctcctcg acggcgtcac gcacgtcatc cacttctccc
     5761 cgctcgcgac gcgcgcgtcc tatcgcaccg ccgcggaagt ctcggaccgg ctcttccgcc
     5821 tccaggggtt cgactgcacg ccgggctcgg gacataggca gctgatactc ccctgcgtcg
     5881 acgcgagcgt cctcacgggc ggccagtacc gcctcgcggc caccgtcacg tcgggtgcat
     5941 tcgtgcgcgc cgccacgccg aggcggcgcg tcgccgtcgt cgtcctcggc gacggggaga
     6001 gcagccccga cgagagcgcc ttcgggcccg ccgaggcgcg cgcgtacctc tcggagatct
     6061 tcgtcccgct cgcggtctgg cgcgtcggca aggcggaggg cggcgcctgg ggcgagggcc
     6121 gccgcgtcac gactccgcag gaattcgtgg acgcgtttgc ccagctgcgc gccgagctcg
     6181 acgcgcagaa gatctgctgg gtcgccgagg acctcgaccc cgcgtccttc cgtctcgcgg
     6241 cgggagacga agggctcgtc ctcgcgggcc gcggtgccgg cgccgcgccg ctcgccgtgg
     6301 tgtcggccgc ggagcccgag gagccgggca cg
//
LOCUS       DAOMFY010000235         6317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4095_length_6317_cov_6.535771, whole genome shotgun sequence.
ACCESSION   DAOMFY010000235 DAOMFY010000000
VERSION     DAOMFY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4095_length_6317_cov_6.535771"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLB01_15445"
     CDS             <1..455
                     /locus_tag="PLB01_15445"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQQ78747.1"
                     /translation="PLPGLGAPRLVLVCPAGEFHDLGARIVAEIGRQEGWQVEFLGAN
                     LPREAAIHCVSMRQPDAVGLSIALAAHVAGCAPLVKEIRRVAPGARVLAGGLAFRLDP
                     ALVPLAGADACVSDAVALRDWLRANRPPARKAPRLPKKLLRKASRTAP"
     gene            537..1781
                     /locus_tag="PLB01_15450"
     CDS             537..1781
                     /locus_tag="PLB01_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ78748.1"
                     /translation="MIGTGISGLSAAWLLSRRHTVRVFDREARPGGHAHTQDAPARGG
                     AVPVDTGFLVFNRETYPNLCRLFEALGTPHHESDMSFSVRCEACSLEWCGTGLSGLFA
                     QPSNLVSPSFLGMLRDIARFNREAPRLLERADADRITLGAFLREGRYGDAMKRHYLAP
                     MAAAVWSSGTLGIEDFPAGLLVRFFRNHGFLGVTTQYRWRTVTGGSRRYVEALTAPFR
                     ERLHLATPVRSLSRDAAGVTLGFADGGTQRFDAAVVATHADEALALLADPSGDEARLL
                     GAWTYSANDTLLHSDASFLPSREKARASWNYRLADCREPGQQATVTYDLNRLQGVPGP
                     EKYLVTLNPGPRVAEGARIATMLYTHPVYTTQSLATHAELPRLSGTRRTYFAGAYFGN
                     GFHEDGLNSGIAVAAALGVAFP"
     gene            1778..2611
                     /locus_tag="PLB01_15455"
     CDS             1778..2611
                     /locus_tag="PLB01_15455"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HQQ78749.1"
                     /translation="MSLPAPSLYTGVVRHERFTPKPHRFQYRVYEALVDVDDLDSLAR
                     KIPFFSHGRPNLTSLHDGDYMGKGEGSIREKANRWLAARGVPPPARLFLLTHLRVLGY
                     AFNPVNYYYAFDEAGRFAFAIAEIHNTFGEGYAYLLERPDGENAETISARFPKAFHIS
                     PFIGMDTTYTFVVTPPGETLRTHVDEFEAGPGGGKFFEAFFAGRRSPLTARSLALALI
                     RHPLLPLRVMAWIHLHAFFLLLKRVPSFTKPAPPAGALYAGAAPRSPFARKRPEARRN
                     A"
     gene            2608..3903
                     /locus_tag="PLB01_15460"
     CDS             2608..3903
                     /locus_tag="PLB01_15460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQQ78750.1"
                     /translation="MSSTPFAEDRSLAATAGAPSAADRLAARLVLRQLEGLEGGALRL
                     VLPDGAVRHFGVPGAGPSARVEVVRWRAFRRLLTGGDLAAAEAFMDGDWKTDDLVAVV
                     RLFIVNADRFDRDTPLARLGNWGNRLLHAARRNSPAGARRNIAAHYDLGNAFYRLFLD
                     ETMTYSCALFSRDDESLEDAQRNKWRRIADLARLGPGVTVLEIGCGWGGFAVWAARTY
                     GCRVTGLTLSTEQRTLAEERAREGGVSDLVDVRLVDYRTLPAEGKRFDRIVSIEMLEA
                     VGFEYLGRFFETCDALLAPDGLAVLQSITVPDHRHDRLLARPDFIKKHVFPGSHVPSL
                     AAIASALSRRTALFVDRLEEIGPHYAETLRRWRTRFLERRAEAAALGFDERFLRMWEY
                     YLAYCEGGFVERYVNDVQFVLTRSGNRSLGPIPAYRGTP"
     gene            3900..4697
                     /locus_tag="PLB01_15465"
     CDS             3900..4697
                     /locus_tag="PLB01_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HQQ78751.1"
                     /translation="MNGPEALRLALLATALALGMMAALWAWHFRLKDAGIVDPGWSLG
                     IGLVALLYALLGSGWPPRRAAVAAMALVWSVRLTVHLARRLHGEPEEGRYRALRAEWS
                     ARGANVSRRFLFFFLFQGLLVVFLSLPMLLAVLNPEPRFSGWEIAGAALWLVSIAGES
                     VADAQLAAFKRDPANRGRTCDAGLWRYSRHPNYFFEWLVWVAWLLFALGSPWGAAAAL
                     GPALMLFFLLRVTGIPATEEQALRSRGDDYRRYQRTTSAFVPWFPKT"
     gene            4694..5725
                     /locus_tag="PLB01_15470"
     CDS             4694..5725
                     /locus_tag="PLB01_15470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQQ78752.1"
                     /translation="MNADRLVASGLVPDFVLRAAIRRLCARRLVEEHAGDAEEVQRRH
                     AAFVAAMRQGPIALETRAANDQHYEVPPAFFKLALGKRLKYSSALFPPGVTSLDAAEE
                     AMLDLTCRRARLEDGQDVLELGCGWGSLSLFMAERFPNSRITGVSNSRDQRGHIEEEA
                     RRRGLANLRIVTADMNDFDPRATFDRVVSVEMFEHMRNWEALFTRVAAWMRPGARFFL
                     HVFSHRDAAYPFVADGEADWMARNFFTGGLMPSDRLAFCFADDLTVEEHWRVSGTHYA
                     RTAEAWLENLDARRAEASAVLAAPGETARARVEAWRVFFMACAELFAFRGGNEWMVSH
                     YLLKRREPG"
     gene            5753..6310
                     /locus_tag="PLB01_15475"
     CDS             5753..6310
                     /locus_tag="PLB01_15475"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ78753.1"
                     /translation="MQDSAADLDLLARIKQGDSAALDALYARYSSPVHSFVWKILRSA
                     EDTEDVVLDVFWQIWRQAGQYDASRGAPPAWIFAVARSRAIDRRRQRHRREDRTISFD
                     DPLTNLDPLDEEAPPDQVASHRQARDLMRDAVANLPPAQREAVELAFFQGLTHVEIAD
                     RLKKPLGTVKTRIRQGLIRLRKTLD"
BASE COUNT          815 a         2385 c         2117 g         1000 t
ORIGIN      
        1 tccccctgcc cggcctcggc gctccccgcc tcgtgctcgt ctgccccgcc ggggagttcc
       61 acgacctcgg ggcgcgcatc gtcgccgaga tcggccgcca ggaaggctgg caggtcgagt
      121 tcctcggcgc gaacctgccg cgcgaggcgg cgatccactg cgtctcgatg cgccagccgg
      181 acgccgtggg actctcgatc gccctcgcgg cgcacgtcgc cggctgcgcg ccgctcgtga
      241 aggagatccg gagggtcgcc cccggcgcca gggtgctcgc gggagggctc gcgttccgcc
      301 tcgacccggc gctcgttccg ctcgccggcg ccgacgcctg cgtgagcgac gccgtcgcgc
      361 tgcgcgactg gctccgcgcg aaccgaccgc ccgcgcgcaa ggccccgcgg ctcccgaaga
      421 agctgctccg caaggcgtcg cgcaccgccc cctgagcggg cacgtccgca cacgctcatc
      481 cggattccgt cgacagacgt attccagtcc gtggaggtgc gctcctggac atcgcggtga
      541 tcgggacggg gatttccggc ctgtcggcgg cctggctgct gtcgcgccgc cacactgtcc
      601 gcgtcttcga ccgcgaggcc cgcccgggcg ggcacgccca cacgcaggac gcgccggccc
      661 gcgggggcgc cgttcccgtg gataccggct tcctcgtgtt caaccgcgag acgtatccga
      721 acctctgccg gctgttcgag gcgctcggca cgccacacca cgagagcgac atgtcgttct
      781 cggtccgttg cgaggcctgc tctctcgagt ggtgcggcac gggcctctcg ggcctcttcg
      841 cacagccctc gaacctcgtc tcgccgtcct tcctcggaat gctccgggac atcgcccgct
      901 tcaaccgcga ggccccgcgc ctcctcgagc gcgcagacgc cgaccggatc acgctggggg
      961 cctttcttcg cgagggccgc tacggcgacg cgatgaagcg gcactacctc gcgcccatgg
     1021 cggcggccgt gtggtcgtcc ggcacgctcg gtatcgagga cttcccggcc gggctcctcg
     1081 tgcgtttctt ccggaaccac ggcttcctcg gcgtgacgac gcagtaccgg tggcggacgg
     1141 tcacgggcgg cagccggcgg tacgtggagg cgctcacggc gcccttccgc gaacggctgc
     1201 atctcgccac gccggtgcgt tccctttccc gcgacgcggc gggcgtgact ctgggtttcg
     1261 cggacggcgg gacgcagcgc ttcgacgccg ccgtcgtcgc cacgcacgcg gacgaggctc
     1321 tcgccctcct ggccgacccc tcgggcgacg aggcgcgcct cctcggcgcg tggacgtact
     1381 ccgcgaacga caccctgctg cattcggacg cgtcgttcct gccttcgcgg gagaaggcgc
     1441 gggcctcgtg gaactaccgt ctcgccgact gccgggagcc cgggcagcag gccacggtga
     1501 cgtacgacct gaaccgcctc cagggcgtgc cggggccgga aaagtacctc gtgaccctga
     1561 accccgggcc gcgcgtggcc gaaggcgcgc ggatcgcgac gatgctctac acccatcccg
     1621 tctacacgac gcagagcctc gcgacgcatg cggagcttcc gcgcctctcc gggacgcggc
     1681 gcacgtactt cgccggcgcg tacttcggaa acggcttcca cgaggacgga ctcaattccg
     1741 gaatcgccgt cgcggcggcc ctcggcgtgg cgtttccatg agcctgcccg ccccttccct
     1801 ctacaccggc gtcgtgcggc acgagcgctt cacgccgaag ccgcaccgct tccagtaccg
     1861 cgtgtacgaa gcgctcgtgg acgtggacga cctcgactcc ctcgcgagga agatcccgtt
     1921 cttctcgcac gggcggccga acctcacgag cctccacgac ggcgactaca tggggaaggg
     1981 cgagggctcg atccgcgaaa aggcgaaccg ctggctcgcg gcgcgcggcg tgcctccgcc
     2041 ggcccggctt ttcctgctga cgcacctccg cgtcctcggg tacgcgttca acccggtcaa
     2101 ctactactac gccttcgacg aggccgggcg cttcgcgttc gcgatcgccg agatccacaa
     2161 cacgttcggc gaggggtacg cctacctcct cgagcgcccg gacggcgaga atgccgagac
     2221 gatctcggcc cgcttcccga aggcgtttca catttcgccg ttcatcggaa tggacacgac
     2281 gtacacgttc gtcgtgacgc ctcccgggga aaccctgcgc acgcacgtcg acgagttcga
     2341 agccggaccc ggaggcggca agttcttcga ggcgttcttc gcgggccggc gctcgccgct
     2401 gacggcccgt tccctcgccc tcgcgctgat ccggcacccg ctcctgcctc tccgcgtcat
     2461 ggcgtggatc cacctgcacg cgttcttcct gctcctgaaa cgcgtcccgt ccttcacgaa
     2521 gcccgcgccg cccgcgggcg ccctctacgc cggggccgcg ccccgatccc cgttcgcacg
     2581 gaaacgcccg gaagcgagga gaaacgcttg agctcgaccc cgtttgccga agaccgttcg
     2641 ctcgccgcca cggccggggc tccctcggcc gccgaccgtc tcgccgcccg cctcgtgcta
     2701 cgacagctgg aggggctcga aggcggcgcc ctccgcctcg ttctcccgga cggcgccgtc
     2761 cggcacttcg gggttcccgg cgcgggcccg tccgcgcgcg tcgaggtcgt ccgctggcgc
     2821 gcgttccgca ggctcctcac gggcggcgac ctcgcggcgg ccgaagcctt catggacggc
     2881 gactggaaga ccgacgacct cgtcgcggtc gtccggctct tcatcgtgaa cgccgaccgc
     2941 ttcgaccgtg acacgcccct cgcgcgcctc ggcaactggg gcaaccgcct tctccacgcg
     3001 gcgcggcgca actcgccggc gggtgcgcgc cggaacatcg cggcgcacta cgacctcggg
     3061 aacgcgttct accgcctctt cctcgacgag acgatgactt attcgtgcgc gctgttctcc
     3121 cgggacgacg agtcgctcga ggacgcgcag cgcaacaaat ggcggcggat cgccgacctc
     3181 gcccgcctcg gcccgggcgt caccgtgctc gagatcggct gtgggtgggg cggcttcgcg
     3241 gtctgggccg cccgcacgta cgggtgccgc gtcaccggcc tcacgctctc gacggagcag
     3301 aggacactgg cggaggagcg ggcgcgggag ggcggggttt cggacctcgt ggacgtccgt
     3361 ctcgtggact accggacgtt gccggcggag ggaaaacggt tcgaccgcat cgtgtcgatc
     3421 gagatgctcg aggcggtggg gttcgagtac ctcgggcgtt tcttcgagac ctgcgacgcc
     3481 cttctcgccc cggacggcct cgccgtcctt cagtcgatca cggtgccgga ccaccggcac
     3541 gaccggcttc tcgcgcggcc cgatttcatc aagaagcacg tcttcccggg cagccacgtg
     3601 ccctcgctcg cggccatcgc ctcggccctc tcgcgccgca cggcgctctt cgtcgaccgg
     3661 ctcgaggaga tcggacctca ctacgccgag acgctgcgcc gctggcgcac gcgcttcctc
     3721 gagcggcgcg ccgaggccgc ggccctcggt ttcgacgagc ggttcctccg catgtgggag
     3781 tactacctcg cctactgcga ggggggattc gtcgagcgct acgtgaacga cgtccagttc
     3841 gtcctgaccc ggtccggcaa ccgctcgctc gggccgattc ccgcctacag gggtacgccg
     3901 tgaacggacc cgaggcgctc cgccttgcgc tcctcgcgac ggccctcgcc ctcgggatga
     3961 tggcggcgct ctgggcgtgg cacttccggc tgaaggacgc cgggatcgtg gatccgggat
     4021 ggtccctcgg catcggcctc gtcgcgctcc tctatgcgct cctcggctcg ggctggccgc
     4081 cgcgccgcgc ggccgtggcc gcgatggcgc tcgtctggtc ggtgcgcctc acggtccacc
     4141 tcgcgcgccg gctgcacggc gagcccgagg agggtcgata ccgggcgctc cgtgccgagt
     4201 ggtccgccag gggagcgaac gtctcgcgga gattcctgtt cttcttcctc ttccaggggc
     4261 tcctcgtcgt cttcctgtcg ctgccgatgc tgctcgccgt tttgaacccc gagccgcgct
     4321 tctcgggctg ggagatcgcg ggagccgcgc tctggctcgt ctcgatcgcg ggcgagagcg
     4381 tcgccgacgc ccagctcgcc gccttcaagc gggatccggc gaatcgcggc aggacctgcg
     4441 acgccgggct ctggcggtat tcccgccacc cgaactactt cttcgaatgg ctcgtctggg
     4501 tggcgtggct cctcttcgcg ctcggctcgc cgtggggcgc cgcggcggcg ctcggcccgg
     4561 cgctcatgct gttcttcctg ctccgcgtga ccggaattcc ggccacggag gagcaggcgc
     4621 tgcggtcgcg cggcgacgac taccgccgct accagcgcac gacgagcgcg ttcgtgccgt
     4681 ggttcccgaa gacatgaacg ccgaccgtct cgtcgcctcc ggcctcgtcc cggacttcgt
     4741 cctccgggcc gcgatccggc ggctctgcgc ccggcgcctc gtggaggagc atgccggcga
     4801 cgccgaagaa gtccagcggc ggcacgcggc cttcgtcgcc gcgatgcggc agggcccgat
     4861 cgcgctcgaa acccgcgccg cgaacgacca gcactacgag gtgccgccgg cgttcttcaa
     4921 gctcgcgctc gggaagcgcc tcaagtactc gagcgcgctg tttcctcccg gcgtcacgtc
     4981 cctggacgcc gccgaggagg ccatgctcga cctcacctgc cggcgcgcgc ggctcgagga
     5041 cggacaggac gtgctcgagc tgggctgcgg ctggggctcc ctttcgctct tcatggccga
     5101 gcggtttccg aactcgcgca tcacgggggt ctcgaactcc cgggaccagc ggggccacat
     5161 cgaggaggag gcccgccgcc gcggcctcgc gaatctccgg atcgtcacgg ccgacatgaa
     5221 cgacttcgac ccgcgggcga cgttcgaccg ggtcgtgtcg gtcgagatgt tcgagcacat
     5281 gcgcaactgg gaggcgctct tcactcgcgt cgcggcgtgg atgcggcccg gcgcgcggtt
     5341 cttcctgcac gtcttctcgc accgcgacgc ggcctacccg ttcgtcgcgg acggcgaggc
     5401 cgactggatg gcccgcaact tcttcacggg cggcctcatg ccctccgacc gcctcgcctt
     5461 ctgcttcgcc gacgatctca ccgtcgagga gcactggcgc gtctcgggca cgcactacgc
     5521 ccgcacggcc gaggcgtggc tcgagaacct cgacgcgcgg cgtgcggaag cgagcgccgt
     5581 gctcgcggcg ccgggcgaaa cggcgagggc gcgcgtcgag gcctggcgcg tgttcttcat
     5641 ggcctgcgcc gagctgttcg cgttccgcgg cgggaacgaa tggatggtgt cgcactacct
     5701 cctgaaacgg cgggaaccgg gctgacttct ctccaacgta agatctgatg cggtgcagga
     5761 ctccgccgcc gacctcgatc tcctcgcgcg gatcaagcag ggagactcgg ccgccctcga
     5821 cgcgctctac gcgcggtact cctcgcccgt ccattcgttc gtctggaaga tcctccggag
     5881 cgccgaggac accgaagacg tcgtcctcga cgtcttctgg cagatctggc ggcaggccgg
     5941 ccagtacgac gcctcgcggg gagcgccgcc ggcatggatc ttcgcggtcg cgcgctcgcg
     6001 cgccatcgac cgccggcgcc agcgccaccg ccgcgaggac cgcacgatct cgttcgacga
     6061 tcccttgacg aatctcgatc ctctcgacga agaggcgcct cccgaccagg tcgcctccca
     6121 ccgccaggcg cgcgacctca tgcgtgacgc cgtcgccaac cttccccccg cgcagcgcga
     6181 ggccgtggaa ctcgcgttct tccagggcct gacccacgtc gagatcgcgg accggctgaa
     6241 gaagccgctg ggcacggtca agacgcggat ccgccagggc ctgatccgcc tgaggaagac
     6301 gctcgactga gggggcc
//
LOCUS       DAOMFY010000236         6231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4182_length_6231_cov_6.605408, whole genome shotgun sequence.
ACCESSION   DAOMFY010000236 DAOMFY010000000
VERSION     DAOMFY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4182_length_6231_cov_6.605408"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /gene="secA"
                     /locus_tag="PLB01_15480"
     CDS             complement(<1..2026)
                     /gene="secA"
                     /locus_tag="PLB01_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQQ78754.1"
                     /translation="MIIDKALSKVFGTKHERDVKAMRPRVAEINALEAGIKPLSDDEL
                     RARVGAIRTRVQDAMKSLPENVEERRALARETLNKELVETFALVREAAWRAIGQRHYD
                     VQLMGGMVLHEGKIAEMRTGEGKTLVATLAVSLNALSGRGVHVVTVNDYLARRDADWM
                     GRIYKFLGFTVGCIQHDMPDEDRRAAYRCDITYGTNNEFGFDYLRDNMKFELAQMVQR
                     GHVFAVVDEVDSILIDEARTPLIISGPSEGETDIYYKVDRIIPKLVRGEEVKDKYGNK
                     TTTGDFLLDEKAHTSSLTEEGVTKCERLLGVENLYDPVNIDVLHACQQALRAHTLYKR
                     DVAYVVKDGQVIIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIERENQTLATITLQNY
                     FRMYDKLSGMTGTADTEAAEFDKIYKLDVVVIPTNRDMVRKDEHDLVYRTEREKFDAV
                     VEEIAEKNEKGQPVLVGTISIEKSELLSTMLKKRKVPHVVLNAKYHEQEATIVAQAGQ
                     VGAVTIATNMAGRGTDILLGGNPEYLARQKAKEDEELYKKLLVELQKETAEAHEQVIE
                     LGGLHIIGTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFGSDRLSGLMK
                     RLGMEEGVPIEHRWVTKSIVRAQGQVEGRNFDTRKHLLEYDDVMNKQREEI"
     gene            complement(2201..3079)
                     /locus_tag="PLB01_15485"
     CDS             complement(2201..3079)
                     /locus_tag="PLB01_15485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQQ78755.1"
                     /translation="MPKREHTIIFVPHARARFRQFHVSSRLLWGLGAAFLTAVFLGAT
                     FSILWVRSINKNREVTAILAENSDLRSRAALTNARLESLEKQLAEFEDRTRRLSIVAG
                     LSAVRDPGTGGVGGLSAAPIDPSASADTGLTEAGRRGALLSSRLQLVEKRLATQADQL
                     ALTPTIAPAAGVLTAGFGQRPDPFTGHPEYHPGIDISTPAGNRIVAPASGTVVKVGVD
                     KGYGRFVQIAHGYGVTTLYGHLQAARVAEGQRVKRGDLVALVGSTGRSTGPHLHYEVR
                     VDGKPNNPLDYVLNAF"
     gene            complement(3244..4770)
                     /locus_tag="PLB01_15490"
     CDS             complement(3244..4770)
                     /locus_tag="PLB01_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQQ78756.1"
                     /translation="MTVARAHSATVVGLDGVPVVVECAVGAGLPGLTLVGLPDAAVKE
                     SRERIRSALRHVGFPAPPKNVVVNLAPADLPKSGTALDLAVALAILAANGDVPAEALD
                     GAMLVGELGLDGALRPVPGVLALAEAARDQGKKILVPSENGPEAAALGGLVVHAADHL
                     GAAIAHLLGKIPLPKSTPTVERTATAAAEPDLSDVRGQAVARRALEIAAAGGHNLLMT
                     GPPGSGKTMLARRLPGLLPPLSREESLEVTRIWSVAGRLPARSGLLVQRPFRSPHHGA
                     SAAALAGGGSDPRPGELSLAHYGVLFLDELPEFRRDALEALREPLEDGVVSVARAAGA
                     RTFPARFLFVAAMNPCPCGHRGDPRRECACSPRDVARYRRKISGPLLDRIDLHVEVPA
                     LSSSDFGADGDGEPSRAVRARVVAAREVQRARTGDPRLVNAALSGRSGRVALRPTPDA
                     KALLERAVDRLTLSARAYQRVLRVSRTIADLAGRTSIGASEVAEALRYRPGRETSESS
                     "
     gene            complement(4784..>6231)
                     /locus_tag="PLB01_15495"
     CDS             complement(4784..>6231)
                     /locus_tag="PLB01_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQQ78757.1"
                     /translation="EDPRDSLVAAAPTASPEEFEKARREIDAVSPGISFDIPIVVNEQ
                     VLRAVAFYQFRIPLGFATALKRSGRYLDLMRGILKEEGVPQDLVYVAMVESAFKYQAH
                     SRAAAHGFWQFIEGTGRRYGLHRTREYDERSDAVKSTRAAAAYFRDLYEMFGDWHLAM
                     AAYDAGEGRILKGLQRTGARDFWELAQGSTLRRETRDYVPFVLATALMAKDPARFGFD
                     VVPDPPIAWDTVTISKPVDLGRVAAAVGATLPELQLLNSELRTRSTPHRVPSYGLRIP
                     PGTSALVVARLPGLPSAPEVAEKRITVKKGETLNKVAARAGVSVAELCDWNDIPRTAK
                     LQKGTVLVVPSRPKHQAKEQLAAKSSNPQGEIRSVPTPASAITRASDVGPFTSAPAAA
                     TPASPAAAAAALPARVDIPAEGFAGAGTAAAGNAAARKVKHTVKPGETLYSLAARYGT
                     TTDAILRENRLRSARSLRAGQTLTLTLAVLN"
BASE COUNT          904 a         2030 c         2302 g          995 t
ORIGIN      
        1 agatctcctc gcgctgcttg ttcatgacgt cgtcgtactc gaggaggtgc ttgcgcgtgt
       61 cgaagttgcg gccctcgacc tggccctggg cgcgcacgat cgacttcgtg acccagcggt
      121 gctcgatcgg gacgccctcc tccatgccga ggcgcttcat gagccccgag aggcggtcgg
      181 atccgaagat ccgcatgagg tcgtcctcga gcgagaggta gaaccgcgag gagccggggt
      241 cgccctggcg gcccgcgcgg ccgcggagct ggttgtcgat gcgccgcgac tcgtgccgct
      301 cggtgccgat gatgtggagc ccgccgagct cgatgacctg ctcgtgcgcc tcggccgtct
      361 ccttctggag ctcgacgagg agcttcttgt agagctcctc gtcctccttc gccttctggc
      421 gcgcgaggta ctcggggttg ccgccgagga ggatgtccgt gccgcggccg gccatgttcg
      481 tcgcgatcgt gaccgcgccc acctggcccg cctgggcgac gatcgtcgcc tcctgctcgt
      541 ggtacttcgc gttcaggacg acgtgcggca ccttccgctt cttgagcatc gtcgacagga
      601 gctcggactt ctcgatggag atcgtgccga cgaggacggg ctgtcccttc tcgttcttct
      661 cggcgatctc ctcgacgacg gcgtcgaact tctcgcgctc ggtccggtag acgaggtcgt
      721 gctcgtcctt gcggaccatg tcgcggttcg tcgggatcac gacgacgtcg agcttgtaga
      781 tcttgtcgaa ctcggcggcc tcggtgtcgg ccgtgcccgt catgcccgac agcttgtcgt
      841 acatgcggaa gtagttctgg agggtgatcg tcgcgagcgt ctggttctcg cgctcgatct
      901 tgaccccttc cttcgcctcg acggcctggt ggagcccgtc ggaccagcgg cgtccgggca
      961 tgaggcggcc cgtgaactcg tcgacgatga tgacctgccc gtccttgacg acgtaggcca
     1021 cgtcgcgctt gtagagcgtg tgcgcgcgca gggcctgctg gcaggcgtgc aggacgtcga
     1081 tgttgaccgg gtcgtagagg ttctcgacgc cgaggaggcg ctcgcatttc gtgacgcctt
     1141 cctccgtcag cgacgaggta tgggccttct cgtcgaggag gaaatcgccg gtcgtcgtct
     1201 tgttcccgta cttgtccttg acctcttctc cgcggacgag cttcgggatg atccggtcga
     1261 ctttgtagta gatgtccgtc tcgccctcgg acgggcccga gatgatgagc ggcgtccggg
     1321 cctcgtcgat gaggatcgag tcgacctcgt cgacgaccgc gaagacgtgc cctcgctgga
     1381 ccatctgcgc gagctcgaac ttcatgttgt cgcgcaggta gtcgaagccg aactcgttgt
     1441 tcgtgccgta cgtgatgtcg cagcggtagg cggcgcggcg gtcctcgtcc ggcatgtcgt
     1501 gctggatgca gccgaccgtg aagccgagga acttgtagat ccgccccatc cagtcggcgt
     1561 cgcggcgggc gaggtagtcg ttgacggtca cgacgtggac cccgcgcccg gacagggcgt
     1621 tcagggaaac ggcgagcgtc gcgacgagcg tcttgccctc gcccgtccgc atctcggcga
     1681 tcttgccctc gtggaggacc atgccgccca tgagctggac gtcgtagtga cgctgcccga
     1741 tggcgcgcca ggccgcctcg cggacgagcg cgaacgtctc gacgagctct ttgttcagcg
     1801 tttcccgggc gagggcgcgg cgctcctcga cgttctcggg aagggacttc atggcgtcct
     1861 ggacgcgtgt gcggatcgcg ccgacgcgcg cgcgcagctc gtcgtccgag agcggcttga
     1921 ttccggcctc gagtgcgttg atctccgcca cgcggggccg catggccttc acgtcgcgtt
     1981 cgtgcttcgt gccgaagact ttagagagag ccttgtcgat gatcatgaaa ggtgggctcc
     2041 gcgcgcttcg cgcgaagaac ggtgagttta gcaggagaag aggagagggg attcttagaa
     2101 gaaggtgaag agggcaaggg tgcgctcgtc agcgcgccgt gccgccgccg tgcaggaccc
     2161 agcagaaaat gccccgaaaa ccaaaggacc cggactccgg tcaaaaagcg ttcagaacgt
     2221 agtccagggg gttattcggc ttgccgtcca cgcgcacctc gtagtggagg tgcgggcctg
     2281 tggaacggcc cgtggatcct acgagggcga cgagatcgcc ccgcttgacg cgctgtcctt
     2341 cggccacgcg cgcggcctgc aggtgcccgt agagcgtcgt gacgccgtag ccgtgcgcga
     2401 tctggacgaa tcggccgtag cccttgtcca cgccgacctt cacgacggtg ccggaggcgg
     2461 gcgcgacgat gcggttgccc gccggcgtcg agatgtcgat cccggggtgg tattcggggt
     2521 ggccggtgaa cgggtcgggc ctctggccga aaccggccgt caggaccccg gccgccggag
     2581 cgatcgtggg cgtgagggcg agctggtcgg cctgagtcgc cagccgcttc tcgacgagct
     2641 gcagccgcga cgagaggagg gcgccgcgac ggccggcctc ggtgagcccc gtgtcggccg
     2701 aagccgaagg gtcgatcggg gccgcggaca ggccgccgac gccaccggta ccggggtcgc
     2761 gtacggccga gaggccggcg acgatcgaaa ggcggcgggt gcggtcctcg aactccgcca
     2821 gctgcttctc gagggactcg agacgggcgt tcgtgagggc cgctcgggag cgcaggtccg
     2881 agttctcggc caggatcgcc gtgacttcgc ggttcttgtt gatggatcgc acccagagga
     2941 tcgagaaggt ggcgcccagg aagacggcgg tcaggaaggc ggcgccgagg ccccagagca
     3001 gcctcgagga gacgtggaac tgcctgaaac gcgctctggc gtgggggacg aagatgatcg
     3061 tgtgctcgcg cttcggcatc tcgtctcgct cgttaccgct cgtgcgttcc gggaaccgcc
     3121 cggacaaaaa aaagcacggc ctacccacga aggccctgag gtgtgcgggc cgaatttgag
     3181 cccctgatgg gagcctgtca agtgaattgg atgcatcatg agaaccatac ggatttcaat
     3241 gacttacgaa gattcggaag tctcccgccc gggcctgtat cgcagcgcct ccgccacctc
     3301 cgaggccccg attgaggttc ggccggcgag gtccgcaatc gtccgggaca cgcgcagaac
     3361 gcgctggtag gcgcgggccg agagcgtcag gcggtcgacg gcgcgttcga gcagggcctt
     3421 ggcgtcgggg gtcggcctca gcgccacgcg ccccgaacgg ccggacaggg cggcgttgac
     3481 gagccgagga tcgcccgtgc gggcccgctg gacctcccgc gcggccacga cccgggccct
     3541 cacggcgcgg gagggctccc cgtcgccgtc cgctccgaaa tcggaagacg agagggcggg
     3601 aacctcaacg tggaggtcga tccggtccag cagcggcccc gagatcttcc ggcggtagcg
     3661 ggccacgtcc cggggggagc aggcgcattc gcgccgcgga tctccccgat gcccgcacgg
     3721 gcaggggttc atggccgcga cgaacaggaa gcgcgcgggg aaggtccgcg cgccggcggc
     3781 gcgggcgacg gacacgacgc cgtcctcgag aggctcgcgc agcgcttcga gggcgtcacg
     3841 cctgaactcc ggcagctcgt cgaggaagag gacgccgtag tgcgcgagtg acagctctcc
     3901 ggggcgcggg tcggaaccgc cccccgccag ggccgcggcc gatgctccgt ggtgcggcga
     3961 gcggaagggg cgctggacga ggagcccgct gcgcgcaggc agcctgcctg cgacgctcca
     4021 gatgcgcgtg acctcgaggc tctcctcgcg cgagagcggc gggaggagtc cgggcaggcg
     4081 ccgcgcgagc atcgtcttgc cggaccccgg cggccccgtc atcagaaggt tgtgtccgcc
     4141 ggcggccgcg atctcgaggg cccgcctcgc gacggcctgg ccgcgcacgt ccgagaggtc
     4201 gggctccgcg gcggcggttg cggttcgttc gacagtcggg gtggatttcg gaagggggat
     4261 ttttcctaga aggtgcgcga tggccgcgcc cagatgatcg gccgcgtgca caacgaggcc
     4321 gccgagtgcc gcagcctccg gaccattctc agaaggaacg agaattttct tgccctggtc
     4381 ccgcgccgcc tccgcgaggg caaggacgcc gggcacgggc cggagggcgc cgtccaaccc
     4441 gagctccccg acgagcatcg cgccgtcgag cgcttcggcc gggacgtcgc cgttcgcggc
     4501 gaggatcgcg agggcgacgg cgagatcgag cgcggtgccg gacttcggaa ggtcggcggg
     4561 cgcgaggttg acgacgacgt tcttcggcgg ggcggggaag ccgacgtggc ggagtgccga
     4621 acggatgcgc tcgcggctct ccttcacggc ggcgtcgggc agaccgacga gcgtcaggcc
     4681 gggcaggccg gcgccgacgg cgcactccac gaccaccggg actccgtcga ggccaacgac
     4741 cgtcgcggaa tgcgcacggg cgaccgtcat ggaggcgccg cggtcagttc aggacggcga
     4801 gcgtgagggt cagcgtctgg cccgcgcgga gcgaccgggc cgaacgcagg cggttttcgc
     4861 ggaggatcgc gtcggtcgtc gtgccgtagc gcgccgcgag cgagtagagc gtctcgccgg
     4921 gcttgacggt gtgcttgacc tttcgggccg cggcgttgcc ggctgcagca gtccccgccc
     4981 cggcgaaacc ctccgccggg atgtcgacgc gcgcgggaag cgccgcggcg gcggcggcag
     5041 gggaagccgg tgtcgccgcg gcgggcgccg acgtgaacgg gcctacgtcc gacgcgcgcg
     5101 tgatcgcgga cgcgggggtc gggacgctgc ggatctcgcc ctgcgggttc gaggacttcg
     5161 cggcgagctg ctctttcgcc tgatgcttcg ggcgcgacgg gacgacgagg acggtcccct
     5221 tctggagctt cgcggtgcgc gggatgtcgt tccagtcgca gagctcggcg acggagacgc
     5281 ccgcgcgcgc ggcgaccttg ttcagagtct cgcccttctt caccgtgatg cgcttctcgg
     5341 caacctcggg cgccgacggg aggccgggca gccgcgcgac gacgagcgcc gaggtgcccg
     5401 gcgggatccg gaggccgtaa gacgggacgc ggtggggcgt cgagcgagtc cggagctcgc
     5461 tgttgaggag ctggagctcc ggcagcgtgg cgccgacggc cgcggccacg cgcccgaggt
     5521 ccaccggctt cgagatcgtc acggtgtccc acgcgatcgg cgggtcgggg acgacgtcga
     5581 acccgaagcg ggccggatcc ttggccatga gagccgtcgc gaggacgaag ggaacgtagt
     5641 cgcgcgtctc ccggcgcagc gtcgagccct gcgcgagttc ccagaaatcg cgggccccgg
     5701 tccgctgaag tcccttcagg atccggccct cgccggcgtc gtacgccgcc atcgcgaggt
     5761 gccagtcgcc gaacatctcg tagaggtccc ggaagtacgc ggccgcggcc cgcgtcgact
     5821 tcacggcgtc gctgcgctcg tcgtattcgc gcgtgcggtg gaggccgtag cggcggccgg
     5881 tgccctcgat gaactgccag aatccgtgcg ccgcggcgcg cgagtgcgcc tggtacttga
     5941 aggcggactc gaccatcgcg acgtacacga ggtcctgcgg gacaccctct tccttcagga
     6001 tgccgcgcat gagatcgagg tagcggccgc tgcgcttgag agccgtcgcg aagccgagcg
     6061 ggatccggaa ctggtagaag gcgaccgcac gcagcacctg ctcgttgacc acgatcggaa
     6121 tgtcgaacga gatgcccggg gaaacggcgt cgatctcccg cctcgccttc tcgaactcct
     6181 cggggcttgc cgtcggcgcc gcggcgacga ggctgtcgcg cgggtcctcc g
//
LOCUS       DAOMFY010000237         6218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4197_length_6218_cov_6.818433, whole genome shotgun sequence.
ACCESSION   DAOMFY010000237 DAOMFY010000000
VERSION     DAOMFY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_6218_cov_6.818433"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..2069)
                     /locus_tag="PLB01_15500"
     CDS             complement(186..2069)
                     /locus_tag="PLB01_15500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQQ78758.1"
                     /translation="MSVPQGSSPFEESIAEFTFKAVAAGVVLGLVFGAANAYLGLRVG
                     LTVSASIPAAVMTVAVFRLFGAKGTILEANLSQTIGSASTSLATGTIFTIPALFLWGV
                     APPYLQVVVLCFLGALLGLAAMIPLRRLLIVDAHAELPYPEGTACAEVLRATAADSSG
                     SAWIFRGMAVGAAVKLIVGLFVLVPNEVGTALPFLPKAEVAVELAPALLAVGFILGYR
                     SSGVLVAGSVVSALVLTPLIAFFGASLSAPLYPETAKLVSAMSAGEIWSRYVRYIGAG
                     AVAAAGILTVLKNLPTMAGAFLAVIRGVRGGGAHAGGRERTDRDLPGSFVFGGIAAVV
                     LVAGLVPGVFAGGMGPLQRAVCAAGVGVFGVLFVAVAARIVGIVGVSSQPTSGITLVT
                     LLGVASLFAAAGWTDGGARAAVLTVGTIVAVAASKAGDISQDLKTGWLVGATPARQQL
                     GQLIGAAFACWGVAATVLLLGSVYTFGTKEIPAPQATLMKTIIEGVLSGALPWGLVLS
                     GAGISVAVILCGVSGLAFAIGVYLPLASMAAIFAGGCARALVERARKGRPAPETDAGV
                     LAASGLVAGEGLVGVVVAALVALGAASKGRPPLAGGLPGEAITFALLLLVCTFLVRAG
                     RAR"
     gene            2169..3629
                     /locus_tag="PLB01_15505"
     CDS             2169..3629
                     /locus_tag="PLB01_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HQQ78759.1"
                     /translation="MSPRKRVIAALAAAASLFLFAEDSSSSSPSSVPSSAFDAAFTRR
                     TMRLDLFHTGGPKGEVVAVAGAGAVDDGPWAGSLTRLLDDTNLGTHFFEVRDPKSNRV
                     LFSRGYSSLYAEWETTPEFKKVSRTFPESLRFPWPKAPVQVVLSRRDSDNVFQPFFST
                     NVDPAAANPAPRAALGKVWTVFENGPATEKLDLLMLGEGYAEKDLPKFHSDVKKLVDL
                     LFSYEPFKSRRADFNVRALDLPSAETGVLRPDSKIFRRTPLSAQYGIFGSERYVLTYD
                     DRALREAAAAAPYDFLEILVNDARYGGGGIFNAQATAAVDSSWAEYLFVHEFGHHFAA
                     LADEYYTSDVAYETGGEKPEPWEPNVTALKDAKKLKWADLVEAGTPLPTPWEKETFEK
                     RSREIQKTRRAIVAAGKPPSALDELFREEAKEETALLGSMKWSGKVGAFEGAAYEAKG
                     LYRSSADCIMFTRDNVGFCPVCRRAITRIIDQYARP"
     gene            complement(3642..4166)
                     /gene="bstA"
                     /locus_tag="PLB01_15510"
     CDS             complement(3642..4166)
                     /gene="bstA"
                     /locus_tag="PLB01_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HQQ78760.1"
                     /translation="MSDLAYPIGKFSWDGPKGAADRALRIDVIAAAPAALRAAVDGLT
                     QAQLDTPYRPGGWTVRQVVHHLPDSHLNAYVRFKLAVTEDVPTVKPYDETAWAELADV
                     KAVPVGTSLALLDALHARWTAFLRSLGDAEWGRTFRHPDLGVVPLEKNLALYAWHGRH
                     HVAHVTALRERMGW"
     gene            complement(4163..4642)
                     /locus_tag="PLB01_15515"
     CDS             complement(4163..4642)
                     /locus_tag="PLB01_15515"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ78761.1"
                     /translation="MDTREQLLESLHRAFDGRSWHGPNLLGAIRGLTPAQAFFRPGPG
                     RHSVYDLILHAAYWKYVARRRLTGEKRGSFPVNGSNFFPEPASKDARAVRDARTLLES
                     MHLELVQVVEALPDAAFSTQHGPWTAGEMIAGVAAHDLYHAGQIQLVKRFFGKRRAR"
     gene            complement(4718..5893)
                     /locus_tag="PLB01_15520"
     CDS             complement(4718..5893)
                     /locus_tag="PLB01_15520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQQ78762.1"
                     /translation="MSAQLREVLTPPLLERCHERAPAYDRENRFCQEDFDDLKAAGYL
                     LMAVPKEFGGAGLTLEEVALETRKLARYAPATALCINMHNYWVGDAADLWRSGDKSCE
                     WILREAVAGEVFAAGHAEHGNDIPGLLSTSKAERVDGGWRITGRKSFGSLTPVWTRLG
                     LHAMDTSDPAAPKIVHAFLPKGTKGVTVKETWDVMGMRATRSDDTVLEGAFVPDKYVA
                     RVVPAGVAGVDMFILGFFTWGLTGFANVYYAIGQRTREIIIEQLKTKTSIGLTRPMIY
                     HPEIQHGIAEISLELESIGPHVDAIARDWTRGAIGPDWFLRLVAMKYHAVEGAFRIVD
                     RALDLSGGFGMFKKSELERLFRDCRAGRFHPANSALVHEIVGKISLGINPDEQPRWG"
     gene            complement(5975..>6218)
                     /locus_tag="PLB01_15525"
     CDS             complement(5975..>6218)
                     /locus_tag="PLB01_15525"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HQQ78763.1"
                     /translation="GRELEARGFPAEERPFAPHLTLARARDPRGSKENARAVAAFAPS
                     VGSFGTQRVDALVLFRSDLGTGPPVHTPIGRFPLGA"
BASE COUNT          990 a         2183 c         2184 g          861 t
ORIGIN      
        1 cgggttgcga agacgaatcg atccttaccc actgccggag cgtaggtggc catgctcgcg
       61 ggtgctgggt cgtcgatctg gtcctcggca aagagtagcc gagaatctcc ccgtggacgc
      121 gctgcgcccg ccgtgggtcg atccacgctc gcaaggatgg cccctcaaag accccggccc
      181 gatcctcagc gcgcgcggcc cgcgcggacg aggaacgtgc agacgaggag caggagcgcg
      241 aacgtgatcg cctcgccggg caggcctccg gcgagcggcg gccggccttt cgacgcggcg
      301 ccgagggcga cgagcgcggc gacgacgacg cccacgagcc cctccccggc gacgaggccg
      361 gaggccgcga ggacgcccgc gtcggtctcc ggggccggcc ggcccttgcg cgccctctcg
      421 acgagcgcgc gggcgcaccc gccggcgaag attgccgcca tcgaggcgag cgggaggtag
      481 acgccgatcg cgaaggcgag gccggagacc ccgcagagga tgacggcgac ggagattccc
      541 gctcccgaga ggacgagccc ccacggcagc gcgccagaca gcacgccctc gatgatcgtc
      601 ttcatgagcg tcgcctgcgg cgccggaatc tccttcgtcc cgaacgtgta cacactcccg
      661 aggaggagga cggtcgccgc gacgccccag catgcgaacg cggcgccgat gagctggccg
      721 agctgctggc gggccggcgt tgcacccacg agccagcccg tcttgaggtc ctgagagatg
      781 tcgccggctt tcgaggccgc gaccgcgacg atcgtcccga ccgtgaggac cgccgcgcgg
      841 gccccgccgt ccgtccagcc cgccgccgcg aagagcgagg ccaccccgag gagcgtcacg
      901 agcgtgatgc ccgacgtggg ctgcgaggag acgccgacga tcccgacgat gcgcgccgcg
      961 accgcgacga agaggacgcc gaagaccccg acgcccgccg cgcagacggc ccgctgaagg
     1021 gggcccattc cgcccgcgaa gacgccgggc acgaggcccg cgacgaggac gacggccgcg
     1081 atgccgccga agacgaacga gccgggcaga tcgcggtccg tgcgctcccg cccgccggca
     1141 tgcgctccgc cgccgcgcac tccgcggatc acggcgagga acgcgcccgc catcgtcggg
     1201 aggttcttca ggacggtcag gatgcccgcc gccgcgacgg cgcccgcgcc gatgtaccgc
     1261 acgtaccgcg accagatctc tcccgcagac atcgcggaca cgagcttcgc ggtctcgggg
     1321 tagagcggcg ccgagaggct cgcgccgaag aacgcgatga gcggggtcag gacgagggcg
     1381 gagacgaccg agcccgcgac gaggacgccc gacgatcggt acccgaggat gaagcccacg
     1441 gcgagaagcg cgggcgcgag ctcgacggcg acctcggctt tcggaaggaa cggcagcgcc
     1501 gtcccgacct cgttcggcac caggacgaag agcccgacga tcagcttgac ggcggccccg
     1561 acggccatgc cgcggaagat ccaggcgctg ccgctcgagt ccgcggcggt cgcgcgcaga
     1621 acctcggcgc aggcggttcc ctccggatac ggaagctccg cgtgggcgtc gacgatcagg
     1681 aggcggcgga gcgggatcat cgcggcgagg ccgaggagcg cgccgaggaa acagaggacg
     1741 acgacctgca ggtagggcgg ggcgacgccc cagaggaaga gcgcggggat cgtgaagatc
     1801 gtccccgtcg cgagggacgt cgacgccgag ccgatcgtct gcgacaggtt cgcctccagg
     1861 atcgtcccct tcgcgccgaa gaggcggaag accgcgaccg tcatgacggc agccgggatc
     1921 gacgccgaga ccgtgagtcc gacgcgcagg ccgaggtagg cgttcgcagc gccgaagacg
     1981 agcccgagca cgacgcccgc ggcgacggcc ttgaacgtga actcggcgat ggactcctcg
     2041 aagggagagc taccctgcgg cacggacacg gtttcaggac tttagcagcc gggccgcgcg
     2101 ctgagcgcgc ggatgaggga agacgcgctt cgcgcgaagt tttcctggaa ggctaatagg
     2161 atgggggcat gagcccacgc aaaagagtga tcgctgccct tgccgcagcc gcctccctct
     2221 tcctgttcgc agaagattct tcgtcttcgt ctccctcttc ggttccctcc tccgccttcg
     2281 acgccgcgtt cacgcggcgg acgatgcgtc tggacctgtt ccacaccggc gggccgaagg
     2341 gcgaggtcgt cgccgtcgcc ggcgcgggcg cggtggacga cgggccctgg gccgggagcc
     2401 tgacccggct cctcgacgac acgaacctcg ggacgcactt cttcgaagtg cgcgacccga
     2461 agtcgaaccg ggtcctcttc tcgcgcggat actcctcgct ctacgccgag tgggagacga
     2521 cgcccgagtt caagaaagtc tcgcggacgt tccccgaatc gctgcgcttc ccgtggccga
     2581 aggcgccggt gcaggtcgtg ctctcccggc gcgattccga caacgtcttc cagcccttct
     2641 tctcgacgaa cgtggacccg gccgccgcga accccgcgcc gcgcgccgcc ctcggcaagg
     2701 tctggaccgt gttcgagaac gggcccgcga ccgagaagct cgacctcctc atgctcggcg
     2761 agggctacgc cgagaaggac ctgccgaagt tccactcgga cgtgaagaag ctcgtcgacc
     2821 tcctcttctc gtacgagccc ttcaagtccc gccgggccga cttcaacgtc cgggcgctcg
     2881 acctgccgtc ggccgagacg ggcgtcctcc gtcccgactc gaagatcttc cggcgcacgc
     2941 cgctctcggc ccagtacggg atcttcggct cggagcgcta cgttctcacg tacgacgacc
     3001 gcgcgctgcg cgaggcagcc gcggcggcgc cgtatgactt cctcgagatc ctcgtgaacg
     3061 acgcgcgcta cggcgggggc ggcatcttca acgcgcaggc gaccgcggcc gtcgactcgt
     3121 cgtgggccga gtacctcttc gtccacgagt tcggccacca cttcgcggct ctcgcggacg
     3181 agtactacac gtccgacgtc gcctacgaga cgggcggcga gaagccggag ccgtgggagc
     3241 cgaacgtgac ggcgctgaag gacgcgaaaa agctgaagtg ggcggacctc gtcgaggccg
     3301 ggacgcctct cccgacgccg tgggagaagg agacgttcga gaagcggagc cgcgagatcc
     3361 agaagacgcg ccgcgcgatc gtcgcggccg gcaagccccc ctcggccctc gacgagctgt
     3421 tccgcgagga ggcgaaggag gagacggctc tcctcggctc gatgaaatgg tccgggaagg
     3481 tcggcgcgtt cgaaggcgcc gcgtacgagg cgaagggcct ctaccgctcg tcggccgact
     3541 gcatcatgtt cacgcgcgac aacgtgggct tctgccccgt ctgccgccgc gcgatcacgc
     3601 ggatcatcga ccagtacgcg cggccgtagg ccgtcctggg gctaccagcc catccgctcc
     3661 ctgagcgccg tgacgtgcgc cacgtggtgg cggccgtgcc acgcgtagag cgcgaggttc
     3721 ttctcgagcg gaacgacgcc gaggtccggg tggcggaacg ttcgccccca ctcggcgtcg
     3781 ccgagcgagc gcaggaacgc ggtccagcgc gcgtgcagcg catcgagcag cgcgagcgac
     3841 gtcccgacgg gcaccgcctt cacgtccgcg agctccgccc acgcggtctc gtcgtacggc
     3901 ttgaccgtgg gcacgtcttc cgtgaccgcg agcttgaacc gcacataggc gttcaggtgg
     3961 ctgtccggca ggtggtggac gacctgccgc acggtccagc ccccggggcg gtagggcgtg
     4021 tcgagctgcg cctgggtgag gccgtcgact gccgcgcgca gagcggcggg cgccgccgcg
     4081 atcacgtcga tcctgagggc gcggtccgcc gctcccttcg gcccgtccca ggaaaacttc
     4141 ccgatcggat acgcaaggtc gctcaccgcg ccctcctctt cccgaagaac ctcttcacga
     4201 gctggatctg ccccgcgtga tagaggtcgt gcgccgcgac gcccgcgatc atctccccgg
     4261 cggtccacgg cccgtgctgc gtggagaacg cggcatcggg cagggcctcg acgacctgaa
     4321 cgagctccag gtgcatgctc tcgaggagag tgcgtgcatc ccgaaccgct cgggcatcct
     4381 tcgacgcagg ttccgggaag aagttcgagc cgttcacggg gaacgagccg cgtttctcgc
     4441 cggtgaggcg cctccgcgcg acgtacttcc agtatgccgc gtggaggatg aggtcgtaga
     4501 ccgagtgacg tccggggccg gggcggaaaa aggcttgcgc gggcgtcagt ccgcggatcg
     4561 ccccgaggag gttgggcccg tgccacgacc gcccgtcgaa ggcgcgatgg agggactcaa
     4621 gaagctgctc ccgagtgtcc atgcgagata actccacgag aggcgcgccg tccgcccctg
     4681 tccattctaa gaaaatctcc ggtccccttc gggcctctca accccagcgc ggctgttcgt
     4741 ccggattgat cccgagcgag atcttcccga cgatctcgtg gacgagcgcc gagttcgccg
     4801 ggtggaagcg cccggcgcgg cagtcgcgga agagccgctc gagctcgctc ttcttgaaca
     4861 tgccgaaccc gcccgagagg tcgagcgcgc ggtccacgat ccggaaggcg ccctcgaccg
     4921 cgtggtactt catcgccacg agcctcagga accagtcggg tccgatcgcg ccccgcgtcc
     4981 agtcgcgtgc gatcgcgtcg acgtgcgggc cgatcgactc gagctcgagc gagatctcgg
     5041 cgatgccgtg ctggatctcc gggtggtaga tcatcggccg cgtcaggccg atcgacgtct
     5101 tcgtcttgag ctgctcgatg atgatctcgc gcgtccgctg tccgatcgcg taatagacgt
     5161 tcgcgaagcc cgtgaggccc catgtgaaga agccgaggat gaacatgtcc acgccggcga
     5221 cgccggcggg aacgacgcgc gcgacgtact tgtcggggac gaacgcgccc tcgaggaccg
     5281 tgtcgtcgct ccgcgtcgcg cgcatgccca tcacgtccca cgtttccttg acggtgacgc
     5341 ccttcgtgcc cttcggcagg aaggcgtgga cgatcttcgg cgcggcggga tcgctcgtgt
     5401 ccatcgcgtg gagcccgaga cgcgtccaga ccggcgtcag gctgccgaag gacttgcggc
     5461 ccgtgatgcg ccagcccccg tcgacgcgct ccgccttcga cgtcgagagg aggcccggga
     5521 tgtcgttgcc atgctcggcg tgtcccgcgg cgaacacctc gcctgccacg gcctcccgga
     5581 ggatccactc gcaggacttg tccccgctcc gccagaggtc ggccgcgtcg ccgacccagt
     5641 agttgtgcat gttgatgcag agcgccgtgg cgggggcgta gcgcgcgagc ttgcgcgtct
     5701 cgagcgccac ctcctccagg gtcaggcctg ctccgccgaa ttccttcggg accgccatga
     5761 ggaggtaccc ggcggccttg agatcgtcaa agtcctcctg gcagaaccgg ttctcacggt
     5821 cgtaggcggg cgcccgttcg tggcatcgct cgaggagggg cggggtgagg acttcgcgca
     5881 gctgggccga catggtccac ctcctgattg atattgcgac tcgatttctg ccacggacgg
     5941 gggccacgcg tcaagggcgg cgtcgccgag gggttcaggc cccgagcggg aaacgtccga
     6001 tcggggtgtg gaccggcggt cccgtgccga ggtcgctccg gaagaggacg agcgcgtcca
     6061 cccgctgcgt gccgaacgaa cccacggacg gcgcgaacgc ggctacggcc cgggcgttct
     6121 ccttcgagcc gcgcgggtcg cgcgcccgcg cgagcgtgag gtgcggtgcg aacggccgtt
     6181 cctccgcagg gaacccgcgc gcctcgagct cgcgcccc
//
LOCUS       DAOMFY010000238         6205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4215_length_6205_cov_7.818699, whole genome shotgun sequence.
ACCESSION   DAOMFY010000238 DAOMFY010000000
VERSION     DAOMFY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_6205_cov_7.818699"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLB01_15530"
     CDS             complement(<1..800)
                     /locus_tag="PLB01_15530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQQ78764.1"
                     /translation="MLWKGFQRPNRLEVEEEKGNYGRFTAQPLERGWGTTVGNALRRV
                     LLSSIEGAAITAVKIEGVEHEFTAVPGVVEDVTDIILNLKGLAIRLHEDGPRSLNLDV
                     KSKGLVTADAFEDDAQLEILNADAPIATLADGGRLRLEATVTKGRGYVPADRNFDAEA
                     PIGTIPLDSAHSPVRRVNYHVEAARVGQATDYDKLVLEVWTNGTITPRDAVGVAAVLL
                     RDHLAIFVNVETDDAAQAAGAEMPQSELDALLDTNVEELELSVRSANCL"
     gene            complement(865..1491)
                     /gene="rpsD"
                     /locus_tag="PLB01_15535"
     CDS             complement(865..1491)
                     /gene="rpsD"
                     /locus_tag="PLB01_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQQ78765.1"
                     /translation="MARYRESVCRLCRREGMKLFLKGDRCFTNKCAVERRNFPPGQHG
                     KRRSKILGYGIQLREKQKVKRFYGVLEGQFRLTFQQAERMRGVTGENLLSLLERRLDN
                     VVHRLGFSGSRPQARQLVRHGHVRVNGKKVNVPSMIVPQGAVVSIKDKSRTNPLIASA
                     VETAKGRGIPAWLELDAAQFQGKVVALPKREDVSIPISEKLIVELYSK"
     gene            complement(1584..2015)
                     /gene="rpsK"
                     /locus_tag="PLB01_15540"
     CDS             complement(1584..2015)
                     /gene="rpsK"
                     /locus_tag="PLB01_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQQ78766.1"
                     /translation="MATPTESTAAAKASAAKGKKKGPKKEKKNIPHALATVQASFNNT
                     IVSITDQQGNVLAWSSAGRIGFKGSRKGTPFAAQLAAGNAAQIAQEHGVRSLDVKVTG
                     PGAGRESAIRALAAAGIDIRSIKDTTPIPHNGCRPRKRRRV"
     gene            complement(2024..2407)
                     /gene="rpsM"
                     /locus_tag="PLB01_15545"
     CDS             complement(2024..2407)
                     /gene="rpsM"
                     /locus_tag="PLB01_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQQ78767.1"
                     /translation="MARIAGVDLPPNKRVEIGLTYIFGIGRPVSNKILVTAKVDRNTR
                     VKDLSEDEVSRIRKTIQDSVTVEGDLRKDTAQNIKRLMEIGCYRGLRHRRNLPTRGQR
                     THTNARTRKGPRKGAVAGKKKATKK"
     gene            complement(2421..2534)
                     /gene="rpmJ"
                     /locus_tag="PLB01_15550"
     CDS             complement(2421..2534)
                     /gene="rpmJ"
                     /locus_tag="PLB01_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQQ78768.1"
                     /translation="MKVRTSVKKICSKCKVIRRAGVVRVICENVKHKQRQG"
     gene            complement(2548..2766)
                     /gene="infA"
                     /locus_tag="PLB01_15555"
     CDS             complement(2548..2766)
                     /gene="infA"
                     /locus_tag="PLB01_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQQ78769.1"
                     /translation="MSKEDAIEVTATVIEPLPNAMFKVELENKHQVLAHISGKMRKHF
                     IRILPGDKVLVELSPYDLTRGRIIYRYK"
     gene            complement(2777..3601)
                     /gene="map"
                     /locus_tag="PLB01_15560"
     CDS             complement(2777..3601)
                     /gene="map"
                     /locus_tag="PLB01_15560"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQQ78770.1"
                     /translation="MITCKGKSEILKMERAARIVHEALAECVAACRPGVTTEEIDRIA
                     AQGIASRGGKAAFPGYRGYPKTICISINDEVVHGIPSPTRALKAGDVVGLDLGAIVEG
                     YYADAARSVAVGPVSDEIKTLVETTHAALLAGIDAVRVGGRIGDIGAAVEAVAKQKKY
                     GVVREFVGHGIGTALHEEPQVPNYGPAGKREAIREGMTLAIEPMFNLGGAAVTTDPDG
                     WTVRTKDRQPSAHFEHTVVATARGPVVLGFGRYASEAILAGAPGAHEYPLPVAVQA"
     gene            complement(3598..4197)
                     /locus_tag="PLB01_15565"
     CDS             complement(3598..4197)
                     /locus_tag="PLB01_15565"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQQ78771.1"
                     /translation="MPSAIVFFGPPGSGKGTQASRLAASLGIPQVSTGDLLRSHVAKG
                     TPLGAIAKPIMESGALVPDDLVTKMLKERLSEKDAAGGAIFDGYPRTVAQAKSLEAIL
                     KDAGGKVDAVLFIDVPDAALVDRLLKRAVLEGRADDTKETIAERLRVYREKTAPLADL
                     YRSAGQLVTIDGDRAVDTVAADVANAVRSSRMTVPKGTA"
     gene            complement(4200..5600)
                     /gene="secY"
                     /locus_tag="PLB01_15570"
     CDS             complement(4200..5600)
                     /gene="secY"
                     /locus_tag="PLB01_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQQ78772.1"
                     /translation="MFESFRNIFAIPDLRKRVLFTFGLLAIYRIGSHIPTPGLDPSAL
                     EEFFKASSGGILGFLDVFSGGALRRLSVFAMGIAPYITASIILQLLTVVWPYLEKLSK
                     EGEMGRRKITQYTRYGTVVLSVVQGAGISLWLWSQTTPSGRYLAVRDPGSESGKLGFI
                     LMAILTLTTGTAFVMWLGEQITERGVGNGISLIIFAGIVANLPTAVLNSIDQIRRGSM
                     SLFTALFLLAFMAAVVAAIVFVERAQRRIPVQYAKRVVGRKVFGGQNTYLPLRVNTGG
                     VIPIIFAVSILQFPQYIASAFKSDFMKKIAEQLAVGQPLYNLFYAAGIIFFCYFYISI
                     IFNPNDTAENMRKYGGFIPGIRPGKATSDYIDSVLTRITLIGAVYLVVVALIPEFLTM
                     GFRVQSIPGIGPWLDAHLPNFVTQGLGVSFFFGGTSLLIVVGVAMDTVQQIESQLVMR
                     NYDGFLKKGRIRGRRG"
     gene            complement(5603..6055)
                     /gene="rplO"
                     /locus_tag="PLB01_15575"
     CDS             complement(5603..6055)
                     /gene="rplO"
                     /locus_tag="PLB01_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQQ78773.1"
                     /translation="MSHGLHNLGPRKGATTGKKRVGRGPGSGLGKTSGKGHKGQNSRA
                     GYSHLRGHEGGQMPLHRRMPKRGFTNIFRKEWATVSVESLERLFAAGENVTVELLRKS
                     GAVKRRGEIKVLGTGEIKKSLVVAAHKFTDGAKKKIEAAGGRCEVLTA"
     gene            complement(6065..>6205)
                     /gene="rpmD"
                     /locus_tag="PLB01_15580"
     CDS             complement(6065..>6205)
                     /gene="rpmD"
                     /locus_tag="PLB01_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004013132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQQ78774.1"
                     /translation="APVDQKQTLFGLGLRRIRQEVVRPDTPAVRGQIHKVRHLVEIVE
                     GA"
BASE COUNT          894 a         2037 c         2146 g         1128 t
ORIGIN      
        1 aggcagttcg cgctgcgcac ggacagctcg agctcctcga cgttcgtgtc gagcagggcg
       61 tcgagctcgc tctgcggcat ctcggcgccg gcggcctggg ccgcgtcgtc ggtctcgacg
      121 ttcacgaaga tcgcgaggtg gtcgcggaga aggacggccg ccacgcccac ggcgtcgcgc
      181 ggggtgatcg tgccgttcgt ccagacctcg aggacgagct tgtcgtagtc ggtcgcctgg
      241 ccgacgcggg ccgcctcgac gtggtagttc acgcggcgga ccggcgagtg ggccgagtcc
      301 agcgggatcg ttccgatggg cgcctcggcg tcgaagttgc ggtcggccgg gacgtagccg
      361 cggcccttcg tcaccgtggc ctcgaggcgc aggcggccgc cgtcggcgag cgtcgcgatc
      421 ggcgcgtccg cgttcagaat ctcgagctgc gcgtcgtcct cgaaggcgtc cgcggtcacg
      481 agtcccttgc tcttgacgtc caggttgagc gagcgcgggc cgtcctcgtg gagtcggatc
      541 gcgaggccct tgaggttcag gatgatgtcc gtgacgtcct cgacgacgcc cggcacggcg
      601 gtgaactcgt gctcgacgcc ctcgatcttg acggccgtga tcgcggcgcc ctcgatcgac
      661 gagaggagga cgcgccggag cgcgttgccc acggtcgtcc cccacccgcg ctcgagcggc
      721 tgggcggtga accggccgta gttgcccttc tcttcttcga cctcgagacg gttgggacgc
      781 tgaaagccct tccacagcat ggtgtcttct cctggccctg cgacttaccc gctgagcggc
      841 gcgcgagagc tcctgttggc agcgttactt ggagtacagc tcgacgatca gcttttcgct
      901 gatggggatc gagacgtcct cgcgcttcgg aagcgcgacg accttcccct ggaactgcgc
      961 ggcgtcgagc tcgagccacg cggggatccc gcggcccttc gcggtctcga ccgcggaggc
     1021 gatgagcggg ttcgtgcggc tcttgtcctt gatggacacg accgcgccct gcggcacgat
     1081 catgctcggg acgttgacct tcttgccgtt cacgcgcacg tggccgtggc ggacgagctg
     1141 gcgggcctgc gggcgagagc ccgagaagcc gagccggtgc acgacgttgt cgagccggcg
     1201 ctcgaggagc gagagcaggt tctcgcccgt gacgccccgc atgcgctcgg cctgctggaa
     1261 cgtgaggcgg aactggccct cgaggacccc gtagaagcgt ttgaccttct gcttctcgcg
     1321 gagctggatg ccgtacccga ggatcttgga gcggcgcttg ccgtgctgac ccggcgggaa
     1381 gttgcggcgc tcgacggcgc acttgttggt gaagcagcgg tcccccttga ggaacagctt
     1441 cattccttcg cgccggcaca gccggcagac cgattcacga taacgagcca cgggacttca
     1501 ggacctccgt cgattccgtc atttgtcgtc tgtccttctt cccggcccgg aatcgagagc
     1561 gcggtttacg gacgtttcgg ctcttagacc cgccggcgct tgcgcgggcg gcagccgttg
     1621 tgcgggatgg gggtcgtgtc cttgatggac cggatgtcga tgcccgcggc ggcgagggcg
     1681 cggatcgcgc tctcgcggcc ggctccgggc ccggtcacct tgacgtccag agagcgcacg
     1741 ccgtgctcct gggcgatctg ggccgcgttg cccgcggcga gctgcgccgc gaacggcgtg
     1801 cccttgcgcg atcccttgaa gccgatgcgg cccgccgacg accaggccag gacgttgccc
     1861 tgctggtccg tgatcgagac gatcgtgttg ttgaaggacg cctgcacggt cgcgagagcg
     1921 tgcgggatgt tcttcttttc cttcttcggg ccttttttct tgcccttggc ggcagaggcc
     1981 ttcgcagcgg cggtggattc agtgggtgtt gccattcatc tacctacttt ttcgtagcct
     2041 tcttcttacc ggccacggcg cccttccggg ggcccttccg cgtgcgggcg ttcgtgtggg
     2101 tgcgctggcc gcgcgtcggg aggttgcggc ggtggcgcag cccgcgatag cagccgatct
     2161 ccatcaggcg cttgatgttc tgcgccgtgt ccttgcggag gtctccctcc accgtgacgg
     2221 agtcctggat cgtcttgcgg atccgcgaca cctcgtcctc ggacaggtcc ttgacccggg
     2281 tgttgcggtc caccttcgcg gtcacgagga tcttgttcga gaccgggcgc ccgatcccga
     2341 agatgtaggt caggccgatt tcgacgcgct tgttcggggg aaggtcgaca cctgcgatac
     2401 gtgccattcg tggttcctct tcagccctga cgctgcttgt gcttgacgtt ctcgcagatg
     2461 accctcacga cgccggcgcg acggatcacc ttgcacttgc tgcagatctt cttgacggac
     2521 gtgcgaactt tcatggtttt cctcggctca cttgtaccgg tagatgatgc gcccgcgggt
     2581 caggtcgtac ggcgagagct cgacgaggac cttgtccccc ggcaggatgc ggatgaagtg
     2641 cttccgcatc ttgccggaga tgtgcgcgag cacctgatgc ttgttttcca gctcgacctt
     2701 gaacatcgcg ttcggcaacg gttcgatcac cgttgcagtc acttcgatgg cgtcttcctt
     2761 cgacatgttc ctccgtctac gcctggaccg ccacgggcag cggatactcg tgggcgccgg
     2821 gggctccggc gaggatggcc tcggacgcgt aacgcccgaa gccgaggacg acggggcccc
     2881 gtgccgtcgc gacgaccgtg tgctcgaagt gggccgacgg ctgccggtcc ttcgtgcgca
     2941 cggtccaccc gtccgggtcc gtcgtgacgg ctgcgccgcc gaggttgaac atgggctcga
     3001 tcgcgagcgt catcccctcg cggatggcct cgcgcttgcc ggcagggccg tagttcggga
     3061 cctgcggctc ctcgtggagc gccgtcccga tgccgtgccc gacgaactcg cgcacgacgc
     3121 cgtacttctt ctgcttcgcc accgcctcga cggccgcgcc gatgtctccg atgcggccgc
     3181 cgacgcggac ggcgtcgatg cccgccagga gcgccgcgtg ggtcgtctcg acgagggtct
     3241 tgatctcgtc ggagaccggc ccgaccgcga ccgaacgggc ggcgtccgcg taatagcctt
     3301 cgacgatcgc gccgaggtcg aggccgacga cgtcgccggc tttcagggcg cgggtcggcg
     3361 acgggatccc gtgaacgacc tcgtcgttga tcgagatgca gatcgtcttc ggatagccgc
     3421 ggtagccggg gaacgccgcc ttgccgccgc gcgaggcgat gccctgcgcc gcgatgcggt
     3481 cgatctcctc ggtcgtgacc cccggacggc acgcggccac gcactcggcg agcgcctcgt
     3541 ggacgatgcg cgcggcccgc tccatcttga ggatctcgga cttgcccttg caggtgatca
     3601 cgccgtcccc ttgggcacgg tcattcgaga ggatcgaaca gcgttcgcca cgtcggccgc
     3661 gacggtgtcg acggcgcggt cgccgtcgat cgtgacgagt tgacccgcgc tccggtagag
     3721 gtccgcgagc ggggccgtct tctcgcggta gacgcggaga cgctccgcga tcgtctcctt
     3781 cgtgtcgtcc gcgcggccct cgaggaccgc gcgcttcagg agccggtcga cgagcgccgc
     3841 atcgggaacg tcgatgaaga ggaccgcatc caccttgccg cccgcgtcct tcaggatcgc
     3901 ttcgagcgac ttcgcctgcg cgaccgtgcg gggatacccg tcgaagatcg ccccgccggc
     3961 ggcgtccttc tcggagagcc gctccttgag catcttcgtg acgaggtcgt ccggcacgag
     4021 cgccccggac tccatgatgg gcttggcgat cgcgccgagc ggcgtcccct tggccacgtg
     4081 gctcctcagg aggtcccccg tcgacacctg gggaatgccc agcgacgccg cgagacgcga
     4141 cgcctgggtc cccttgcccg atccgggagg tccgaagaac acgatggcgg acggcatggt
     4201 taccccctgc ggccccggat gcggcccttc ttcaggaagc cgtcgtagtt ccgcatgacg
     4261 agctgcgatt cgatctgctg gaccgtgtcc atcgcgacgc cgaccacgat gaggagcgac
     4321 gtgccgccga agaagaacga gacgccgagg ccctgcgtca cgaagttcgg caggtgcgcg
     4381 tcgagccacg ggccgatgcc cgggatgctc tggacccgga agcccatcgt caggaactcg
     4441 ggaatgagtg cgacgaccac gaggtagacc gctccgatga gcgtgatccg cgtgagcacc
     4501 gagtcgatgt agtccgacgt ggccttcccg ggacggatgc ccgggatgaa gccgccgtac
     4561 ttgcgcatgt tctcggcggt gtcgttcggg ttgaagatga tcgagatgta gaagtagcag
     4621 aagaagatga tccccgccgc gtagaagagg ttgtagagcg gctggccgac ggcgagctgc
     4681 tcggcgatct tcttcatgaa gtcgctcttg aacgcggacg cgatgtactg ggggaactgc
     4741 aggatcgaga cggcgaagat gatcggaatg acgccgcccg tgttcacgcg caggggcagg
     4801 tacgtgttct gcccgccgaa gaccttgcgg ccgacgacgc gcttggcgta ctggaccggg
     4861 atgcgccgtt gcgcgcgttc gacgaagacg atagctgcta cgaccgccgc catgaaggcg
     4921 agcaggaaga gggccgtgaa gaggctcatg gagccgcggc ggatctggtc gatcgagttc
     4981 aggacggccg tcgggaggtt cgcgacgatg ccggcgaaga tgatgagcga gatgccgttg
     5041 cccacgcccc gctccgtgat ctgctcgccg agccacatga cgaacgcggt gcccgtcgtg
     5101 agcgtcagga tcgccatcag gatgaacccg agctttccgg actccgaacc ggggtctcgc
     5161 acggcgaggt agcggccgct gggcgtcgtc tggctccaca gccaaagcga gatgcccgcg
     5221 ccctggacga ccgaaaggac gaccgtcccg tagcgggtgt actgcgtgat cttccgccgg
     5281 cccatctccc cttccttcga gagcttctcg aggtagggcc agacgaccgt caggagctgc
     5341 aggatgatcg acgccgtgat gtacggcgcg atgcccatcg cgaacacgga gagccggcgc
     5401 agcgcgcctc ccgagaacac gtcgaggaag cccaggatgc ctcccgagct cgccttgaag
     5461 aattcctcga gcgccgacgg gtccagaccc ggcgtcggga tgtgcgaacc gatccggtaa
     5521 atcgcgagga ggccgaacgt aaagaggacg cgcttcctca ggtccgggat cgcgaagatg
     5581 ttgcggaacg actcgaaaat tttcacgccg tcaggacctc gcagcgtccg ccggcggcct
     5641 cgatcttctt cttggctccg tcggtgaact tgtgggccgc gacgacgagg ctcttcttga
     5701 tctcgcccgt cccgagcacc ttgatctcgc cgcggcgctt gaccgcgccg gacttgcgga
     5761 ggagctcgac ggtgacgttc tcacccgccg cgaaaaggcg ctcgagcgac tcgacgctca
     5821 ccgtcgccca ttccttgcgg aagatgttcg tgaagccgcg cttgggcatg cgccggtgga
     5881 gcggcatctg gccgccctcg tggccgcgca ggtgcgagta gcccgcgcgg gagttctggc
     5941 ccttgtggcc cttccccgac gtcttgccga ggccggagcc cgggccgcgt cccacgcgct
     6001 tcttgcccgt ggtcgcgccc ttgcggggtc cgaggttgtg cagtccgtgc gacatggcgt
     6061 tcctttacgc cccctcgacg atttcgacga ggtggcgaac tttgtggatc tggccgcgga
     6121 cggccggggt gtccggacgg acgacttcct gccggatgcg gcggaggccg aggccgaaca
     6181 gcgtctgctt ctggtcgacg ggcgc
//
LOCUS       DAOMFY010000239         6194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4228_length_6194_cov_9.979475, whole genome shotgun sequence.
ACCESSION   DAOMFY010000239 DAOMFY010000000
VERSION     DAOMFY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4228_length_6194_cov_9.979475"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PLB01_15585"
     CDS             complement(<1..864)
                     /locus_tag="PLB01_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQQ78775.1"
                     /translation="MGLLDNLKNQIGNQLGSQFIEIIQWLDDSNDAMVYRFPVYNQEI
                     KMGAQLTVRENQAALFINEGKAADLFGPGRHELSTQNLPILTTLRGWKYGFQSPFKAE
                     VYFFNTRLFTDLKWGTTNPVMMRDAEFGMIRLRAFGTYAMKIADPKTFFATIVGTQGL
                     TTTEEITGQLRSTILSRLSDAIAEAKVSALDIASKYDELSGNGKQILAPEFSSFGLDL
                     SKFFIENVSLPEEVEAAIDQRTKLGVLGDKMGQYTQLQAADAMKIAAANPGGAAGAGV
                     GIGAGIAMGGMM"
     gene            complement(960..1619)
                     /locus_tag="PLB01_15590"
     CDS             complement(960..1619)
                     /locus_tag="PLB01_15590"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006454099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQQ78776.1"
                     /translation="MARLVDLSHTVRAGMATYKGLPGPVITDHLSREASRPLYAGGTT
                     FQIGKIEMVANTGTYVDAPSHRWEDGVDLSGLSLDLLAGREGICIAAPHARGLAIGED
                     FLEGKEVGGKAVLLCTGWASRWGTPGYQERSPHLTLEASSLLVSRGAAIVGIDSHNID
                     DTEDLTRPAHSILLRAGIPVVEHLTNLEALPPSGFRFFAVPVKVAGMGTFPVRAFAEV
                     P"
     gene            complement(1522..2928)
                     /locus_tag="PLB01_15595"
     CDS             complement(1522..2928)
                     /locus_tag="PLB01_15595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQQ78777.1"
                     /translation="MAFTSFGLHPDLLRGVKEMGFTRPTPIQSDSIPPAMAGRDLLAC
                     AMTGSGKTAAFVLPILHRLMGKPRGTTRALILTPTRELAAQIDEHLKQLAVHTAMSGA
                     AVFGGVGMGPQEHAFRSGVDVIVATPGRLLDHFRFPYAKLAGLEVLVLDEADRMLDMG
                     FLPDIRRVLKHLPPRSQTLFFSATMPPPIAQLSKEMLKDPVLVNIERQSLPAVGITQA
                     VYPVPQDLKFALLLALMKRGDFKSVLTFTRTKHRANRLADQLTRHGVACERIHGNRSQ
                     AQRTAALAGFKDGKFKVLVATDIAARGIDVEALSHVVNFDVPHVPEDYIHRVGRTARA
                     EMTGDAFTFVAPDEEADLRQIERAIGKRLPRVTVPDFDYTTRPVERFEVPIAERIAAI
                     RARKAEERKRAKEKAERRLQHEAAMAGRGRPAAPRPGAQPVSSRHGAPRGPQPHGPRR
                     DGHVQGPARPGHHRPPQS"
     gene            3202..6081
                     /locus_tag="PLB01_15600"
     CDS             3202..6081
                     /locus_tag="PLB01_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ78778.1"
                     /translation="MIGTTLSHYRILEKIGEGGMGVVYRAVDTRLDRTVALKVLRPET
                     VGDPERKWRFIREAKAASALNHPHIVTIHDVDADQGVDFMVMEYVEGTPLNRLILPGG
                     LPVREAVDIAVRIASALGAAHAAGIVHRDVKPANVVVGPGGRVKVLDFGLAKLVERGP
                     GPDDMTSDSTATAASIIERTREGVILGTVAYMSPEQAEGKPLDARSDVFSLGAVIHEM
                     LTGRRPFQGDSYLRTLTAILRDPPPALAAVRPDVPSGLEKIVTRALAKRPEDRFSNAG
                     DVEKELLALGGGASTAAGSPRPLWKRPASVLTACVLLAVVGVGAWRLAETARVRRVRE
                     KTLPEIARLVEQQRFTEAIRLAREAEKTLPEEVARLKTSWFALTVRTDPPGAEAFFAH
                     YPEGDSAWIPAGKTPLEGYRLPIAHYRWRFTLPGYQTVEGTTESWSKVLRLDPVAAAP
                     PGMVRVPGGPFSLRSLPAVTLGEFFLDRTEVTNRQYKAFVDAGGYTKREYWRPPFVKD
                     GRTLSFEEAVAAFRDATGRPGPATWELGTYLEGREDFPVSGVSWYEAAAYAEFAGKAL
                     PTVYHWYQATGNGNFSDILRLSNFGGKGPAAVASLKGMGPYGTYDMAGNVKEWCWNES
                     GGRRYLLGGAFSDAVYAYSEPDAQAPFERSPLYGFRCARYEAPLPEEQLREVPVLARD
                     FSMEKPVPDDVFRVFQGFYAYDKGPLDVKSEGVDDASPYWRTEKVSYLAASGERIPAF
                     LFLPRNARPPYQTVVYFPSSQARVTPSSADLDIRFLDFVIRSGRALLHPIYNDTYERR
                     LAVRPAGPASFRDLRIQWSKELGRSIDFIETRPDLDKGALAFYGLSLGAIDGVTFVAL
                     EPRIRTAVLAAGGLRFMRVPPEVEPYNFAPRIRIPVLLIAGRYDFGHPYETMQVPLFR
                     ALGTPEKDKKHVTFEGGHIPPKIQSLIKEILDWLDRTLGPVPVTG"
BASE COUNT          939 a         2092 c         2196 g          967 t
ORIGIN      
        1 catcatgccg cccatcgcga tgccggcgcc gatcccgacg cccgcgcccg cggcgccgcc
       61 cgggttcgcg gcggcgatct tcatcgcgtc cgcggcctgg agctgcgtgt actgccccat
      121 cttgtcgccg aggacgccca gcttcgtccg ctggtcgatc gcggcctcga cctcctcggg
      181 gagcgagacg ttctcgatga agaacttcga gaggtcgagc ccgaagctcg agaactcggg
      241 cgcgaggatc tgcttgccgt tgcccgagag ctcgtcgtac ttcgaggcga tgtcgagcgc
      301 ggagaccttc gcctcggcga tcgcgtccga cagcctcgac aggatcgtcg agcgaagctg
      361 gccggtgatc tcctccgtcg tcgtgaggcc ctgggtcccg acgatcgtcg cgaagaacgt
      421 cttcgggtcc gcgatcttca tcgcgtacgt tccgaacgcg cgcaggcgga tcattccgaa
      481 ctcggcgtcg cgcatcatca cggggttcgt cgtgccccac ttgaggtccg tgaagagacg
      541 cgtgttgaag aagtagacct cggccttgaa gggcgactgg aagccgtact tccacccgcg
      601 cagcgtggtc aggatcggaa ggttctgcgt cgagagctcg tggcggcccg ggccgaacag
      661 gtccgccgcc ttgccctcgt tgatgaagag tgcggcctgg ttctcgcgaa ccgtgagctg
      721 cgcgcccatc ttgatttcct ggttgtagac cgggaaccgg tagaccatcg cgtcgttcga
      781 gtcgtccagc cactggatga tctcgatgaa ctgcgagccg agctggttgc cgatctgatt
      841 cttgagattg tcgagcaggc ccacgaggaa cctccttcgg cgattgaaaa gtggacgcta
      901 cagtctatcc aaatatacga acgggggccc ctccgggttc accgttgcgg gcgaaggagt
      961 tacgggacct cggcgaacgc gcgcacgggg aacgtcccca tcccggccac cttcacgggc
     1021 accgcgaaaa accggaaacc ggacggcggc agcgcctcga ggttcgtgag gtgctcgacg
     1081 acggggatcc cggcccgcag caggatcgag tgcgccggcc gcgtgaggtc ctccgtgtcg
     1141 tcgatgttgt gcgagtcgat cccgacgatg gcggccccgc gggagaccag gagagaagat
     1201 gcttcgaggg tcagatgggg cgagcgctcc tggtatccgg gagtgcccca gcgagacgcc
     1261 cagcccgtgc aaagcagcac ggccttgccc ccgacctctt taccttctaa gaaatcttct
     1321 ccgatggcga gcccccgcgc atgcggcgcc gcgatgcaga tcccctcgcg gcccgcgagc
     1381 aaatcgagcg agagcccgga gagatccacc ccgtcttccc atcggtgaga gggcgcgtcc
     1441 acgtacgtgc ccgtgttcgc gaccatctcg atcttcccga tctggaacgt cgtcccgccc
     1501 gcgtagagcg ggcgggaggc ctcacgactg aggtggtctg tgatgaccgg gccgggcagg
     1561 cccttgtacg tggccatccc ggcgcggacc gtgtggctga ggtccacgag gcgcgccatg
     1621 ccgcgaggat accggctgcg cccctgggcg cggcgcggcg ggacggccgc ggccggccat
     1681 cgcggcctcg tgctgcaagc gcctctcggc cttctccttg gccctcttcc gctcctcggc
     1741 cttgcgggcg cggatcgcgg cgatgcgttc ggcgatcgga acctcgaagc gctccacggg
     1801 acgcgtcgtg tagtcgaagt ccggcaccgt cacgcgcggc aggcgcttgc cgatcgcgcg
     1861 ctcgatctgg cggaggtccg cctcctcgtc cggcgcgacg aacgtgaacg cgtcgcccgt
     1921 catctcggcg cgcgccgtgc ggccgacgcg gtggatgtag tcctcgggga cgtgcgggac
     1981 gtcgaagttc acgacgtgcg agagcgcctc gacgtcgatg ccgcgcgccg cgatgtccgt
     2041 cgcgacgagg accttgaact tcccgtcctt gaagcccgcg agcgcggccg tgcgctgcgc
     2101 ctggctgcgg ttgccgtgga tgcgctcgca cgcgacgccg tggcgcgtga gctggtccgc
     2161 gaggcggttc gcgcggtgct tcgtgcgcgt gaacgtgagg acgctcttga agtcgccgcg
     2221 cttcatgagc gcgaggagga gcgcgaactt gaggtcctgc gggaccgggt agaccgcctg
     2281 cgtgatcccg acggccggca gcgactgccg ctcgatgttc accaggaccg ggtccttgag
     2341 catctccttc gagagctgcg cgatcggtgg cggcatcgtg gccgagaaga acagagtctg
     2401 gctgcgcggg ggcaggtgct tgaggacgcg gcggatgtcc gggaggaacc ccatgtcgag
     2461 catccggtcc gcctcgtcga ggacgaggac ctcgaggccg gcgagcttcg cgtacgggaa
     2521 gcggaagtgg tcgagaaggc gcccgggggt cgcgacgatt acgtcgacgc cgctccggaa
     2581 cgcgtgctcc tgcgggccca tgccgacgcc gccgaagacg gccgcgcccg acatcgccgt
     2641 gtggacggcg agctgcttca agtgctcgtc gatctgcgcg gcgagctcgc gcgtgggggt
     2701 gaggatcagc gcgcgcgtcg tgccccgcgg cttgcccatg agacggtgaa ggatcgggag
     2761 gacgaacgcg gccgtcttgc cgctgcccgt catcgcgcag gcgaggagat cgcggcccgc
     2821 catcgcgggc gggatggaat cgctctggat gggcgtggga cgggtgaagc ccatctcctt
     2881 gacgccgcgg agaaggtcgg ggtggagacc gaacgaggtg aaggccatgt ggtgagggat
     2941 cgctttccgg gcccggccgc cgaggcgcac cgttcccgtg tggggtccgg cggggcgggg
     3001 aacgatggat cgccggcggg ccatcgagcg gaccggggcg gccgtttccc gtgcggaggg
     3061 cggcggcggg tcctgtgggg ttcggggccg ggacgcgttc gcgcccgtca gccggacgtc
     3121 agggtctcac ggagggcccg atccgtcaaa cgcggagccg aagcggtaaa gtcgcgcgaa
     3181 aggaaacacg ttctcaaact catgatcggg accacgctct cgcactaccg catcctcgag
     3241 aagatcggcg agggcgggat gggcgtcgtc taccgcgccg tcgacacgcg cctcgaccgg
     3301 acggtcgctc tcaaggtcct ccggccggaa accgtcggcg accccgagcg caagtggcgc
     3361 ttcatccggg aggcgaaggc cgcgtcggcc ctgaaccacc cgcacatcgt cacgatccac
     3421 gacgtcgacg cggaccaggg cgtcgacttc atggtcatgg aatacgtcga ggggacgccg
     3481 ctcaaccggc tgatcctgcc cggcggcctg cccgtccgcg aggccgtcga catcgccgtc
     3541 cggatcgcgt cggctctcgg tgcggcgcac gcggcgggca tcgtccaccg cgacgtgaag
     3601 cccgcgaacg tcgtcgtggg tccgggcggg cgcgtgaagg tgctcgactt cggcctcgcg
     3661 aagctcgtcg agaggggccc cggcccggac gacatgacga gcgactcgac cgcgaccgcc
     3721 gcgtcgatca tcgaacgcac gcgcgagggc gtgatcctcg ggacggtggc gtacatgtct
     3781 cccgagcagg cggaggggaa gcccctcgac gcgcgctcgg acgtcttctc gctcggcgcc
     3841 gtgatacacg agatgctcac gggccggcgg ccgttccagg gcgactcgta cctccggacg
     3901 ctcacggcga tcctccgcga cccgccgccg gcgctcgcgg ccgtgcggcc cgacgtgccg
     3961 tctgggctcg agaagatcgt cacgcgcgct ctcgcgaagc gtccggaaga ccggttttcc
     4021 aacgccggag acgtcgaaaa ggaactcctc gcgctcggtg gcggcgcatc gaccgcggcc
     4081 ggatcgcccc ggcctctctg gaaacgcccg gcctccgtcc tgaccgcctg cgttcttctg
     4141 gccgtggtcg gtgtcggcgc atggcggcta gcagagacag cgcgcgtgcg ccgcgttcgc
     4201 gagaagacgt tgcccgagat cgcgcggctc gtggagcagc agagattcac ggaggccatc
     4261 cgcctcgcgc gggaggccga aaagacgctg cccgaggaag tcgcgcggct caagacgagc
     4321 tggttcgcgt taacggtccg gaccgacccg ccgggcgccg aggccttttt cgcgcactat
     4381 cccgagggcg actcagcctg gataccggcg gggaagacgc cgctcgaggg gtaccgtctc
     4441 ccgatcgcgc actaccggtg gcgcttcacg ctgcccggct accagacggt ggagggcacg
     4501 acggagtcct ggtcgaaggt gttgcggctc gaccccgtcg cggccgcgcc gccggggatg
     4561 gtgcgcgtgc cgggcgggcc tttctccctg cgcagcctcc cggccgtgac gctcggcgag
     4621 ttcttcctgg accggaccga ggtcacgaac cgccagtaca aggcgttcgt agacgccggg
     4681 ggatacacga agcgagagta ctggaggccg cccttcgtca aggacggccg gacgctctcg
     4741 ttcgaggagg cggtcgcggc cttccgcgac gcgacgggcc gcccgggccc tgcgacgtgg
     4801 gagctcggaa cgtacctgga gggccgcgag gacttcccgg tgagcggcgt ctcctggtac
     4861 gaggccgcgg cctacgccga gtttgccggc aaagccctgc cgacggtcta ccactggtac
     4921 caagcgaccg ggaacggaaa tttctcggac atcctccgcc tgtccaactt cgggggaaag
     4981 ggacccgcgg ccgtcgcgag cctcaagggc atgggccctt acggaacgta cgacatggcg
     5041 gggaacgtca aggagtggtg ctggaacgag tccgggggcc gccggtacct cctcggcggc
     5101 gcgttttccg acgcggtcta cgcgtactcc gagccagacg cgcaggctcc gttcgagcgt
     5161 tccccgctgt acgggttccg ctgcgcccgg tacgaggcgc cgctccccga ggagcagctc
     5221 cgcgaggtcc ccgtcctcgc gcgagacttc tcgatggaaa agcccgtgcc ggacgacgtc
     5281 ttccgcgtct ttcagggctt ctacgcgtac gacaaggggc cgctcgacgt gaagagcgag
     5341 ggtgtagacg acgcctctcc gtactggcgg acggagaaag tcagctatct tgcggcctcc
     5401 ggcgagagga tccccgcgtt cctcttcctc ccgaggaacg cgcgcccgcc gtaccagacc
     5461 gtcgtgtact tcccgagcag ccaggcgcgc gtcacgccgt cgagcgcgga cctcgacatt
     5521 cggtttctcg acttcgtgat ccggagcggc cgggcgctgc tccacccgat ctacaacgac
     5581 acgtacgagc ggcgcctcgc ggtgcggccc gcggggcccg cgtccttccg cgacctgagg
     5641 atccagtggt ccaaggagct gggacgctcg atcgacttca tcgaaacgcg gcccgacctc
     5701 gacaagggtg cgctcgcgtt ctacgggctg agccttggcg cgattgacgg cgtgaccttc
     5761 gtcgcgctcg agccgcggat caggacggcg gtcctggcgg cgggagggct ccggttcatg
     5821 cgggtcccgc ccgaagtcga gccctacaac ttcgcgccgc gcatccggat cccggtcctc
     5881 ctcatcgccg ggcggtatga cttcgggcat ccctacgaga cgatgcaggt tccgctcttc
     5941 cgtgccctcg gaacgcccga gaaggacaag aaacacgtca cgttcgaggg cggccacatc
     6001 cctccgaaga tccagtccct catcaaggaa atcctcgact ggctggaccg gacgctcggc
     6061 cccgtgcccg tgacgggctg acgcggcccc gtcagcgcgc gggcgtcggc cgcgtctcga
     6121 cgacgtcgac gagcgggctc gcgaagaggc gccgcgcggg cccgagctgc agttcgcggt
     6181 catcgattcc gggc
//
LOCUS       DAOMFY010000240         6194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4229_length_6194_cov_6.900472, whole genome shotgun sequence.
ACCESSION   DAOMFY010000240 DAOMFY010000000
VERSION     DAOMFY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4229_length_6194_cov_6.900472"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PLB01_15605"
     CDS             <1..844
                     /locus_tag="PLB01_15605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HQQ78779.1"
                     /translation="DGGFAVRLADAPDVRASRVILATGGLSVLGGGADGRGFAWAEAL
                     GHTLVPTYPALVPLTGDAPAHHALSGVSVNARVTATCAASGEKAGSEGGFLFTHRGWS
                     GPAVLDVSHIVERAARADARAEVNICFGFGEEEISANGPVRDRWDESLRVGGGAASSL
                     SSFLRKSSSLPDRVIQLVLQEAGVADQPLARLTRDARRRLAQTLAAFPIPATGTEGYR
                     TAEVTGGGVALDEVDPGSGRSKVVPGLWLAGEVLDAFGPIGGFNFQWAWGTGWTAGVS
                     AARP"
     gene            complement(846..1529)
                     /locus_tag="PLB01_15610"
     CDS             complement(846..1529)
                     /locus_tag="PLB01_15610"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQQ78780.1"
                     /translation="MPTRVFLVRHGATTLSAEDRFAGETEVELSEEGREQLHHLAARL
                     AREPLAAVCASPMRRTLESARILAAPHGLSVLPVPALREISHGRWEARTRTEVEALWP
                     DEYACWERDPYTFAPKGGETGLAVTARALPALVSLVNEHAGRSILVVSHKATIRLLLS
                     ALIGFDPRTYRDRLDQSPACLNVLDFKNPAQARLTLFNDVSHYAHAGTSTPGLPKGRL
                     SKWWDEPPI"
     gene            complement(1535..1888)
                     /locus_tag="PLB01_15615"
     CDS             complement(1535..1888)
                     /locus_tag="PLB01_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013796265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78781.1"
                     /translation="MIRRDPVRLVYSTASGRVCATCGWPADECKCSSKEEEKVPAKVV
                     VKLRLEKKGRGGKTVTVLDGLPRNEDFLEGLAKDLKKSLGTGGSVVGATIELQGEWRE
                     RLRILLPSKNLLVKG"
     gene            1910..2341
                     /gene="arfB"
                     /locus_tag="PLB01_15620"
     CDS             1910..2341
                     /gene="arfB"
                     /locus_tag="PLB01_15620"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQQ78782.1"
                     /translation="MADPISAPPGVFVPPAALEVHAVRSGGPGGQNVNKVASKILLEV
                     DLAKIEGLSDLARRRLATLAGRRLGADGILRVTAQDSRDQYRNLAAARDKVRELLEQA
                     QVAPKARRATRPSRAAKEKRLKAKRVAAIRKARRGSVRDED"
     gene            2357..2860
                     /locus_tag="PLB01_15625"
     CDS             2357..2860
                     /locus_tag="PLB01_15625"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQQ78783.1"
                     /translation="MNRKLAFALFAAALFAPVPARAQAKADLQVTAFGFTGPTANTVP
                     KPSCEPNTVVYSFAVVVTNAGAGPSPSSASLGGRPLLTVAAQDRAGWMASVPLPEIAP
                     GKSVSVNADVLFLASDPAYMVKANHPFLATVDPGNLVDEADEANNTKGPLTMGPPAGC
                     ERFFKKK"
     gene            complement(2864..3259)
                     /locus_tag="PLB01_15630"
     CDS             complement(2864..3259)
                     /locus_tag="PLB01_15630"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQQ78784.1"
                     /translation="MDTNAHEPAIRVSMLPRDTNGHGTIFGGVLLAYIDQAGAIATRP
                     FCNLVVTVKMNEVVFHHPVYVGDVVSFYAKVVKIGTTSISVHVTVTAERWKESGKVLK
                     VTEAEVVYVNVGEDGRPVPIRKPASGAWA"
     gene            3294..5471
                     /locus_tag="PLB01_15635"
     CDS             3294..5471
                     /locus_tag="PLB01_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQQ78785.1"
                     /translation="MPDTKTALFLGRAVDPSTGKTGREDFRLDPSDLTTHGLIVGMTG
                     SGKTALGIVLIEELLQRGIPVLAVDPKGDLGNLLLNFPGLAPEEFAPFVDPSAGTPES
                     EAKKWTDGLAGWGLGPADVKALLASRDAVVYTPGSTAGVPLDLFGACTPPLAGADEED
                     GRDLVGSWVGGLLGLAGRDADPVKSRDFVFLAACVDALWADGRTATLETLLDTASSPP
                     FAKVGALSLDAFYPAKDRQALVLALNGLLASPSTAAFRQGDALDVAAMLAPAKDGRAR
                     LSIVSIAHLGDAERLFVVATLLARVKSWMRTLPGSPALRALVYVDEIFGFFPPTAEPP
                     TKRPLLTLLKQARAFGVGVVLASQNPVDLDYRGLANCGCWWVGTLQTERDRNRLSEGL
                     VEAGGRDASKLLDRTRKRVFLLHDVHRKEPALVETRWAMSYLRGPMTKSDLAKLRGSA
                     PAPSKKAEAAPAGSAPPPLPQEWPARWVDRRGADLASPALYVRYAVRYKTAKGTSPET
                     QGVKLFPLSAPTAADVLEGEATDMAADGLLDKAPRALRYPDLPAWVGPAGVKAAEKAV
                     RSRLPDKLATTLLRDPVTGALSLPGETPDAFAARAASGAEAPAALRERLEKKKRDLAA
                     AEATEQGRSMETYASIGTAALDVLGGLLGKKKTLRVSKVGSVLSKKRMEGTAEAKVEG
                     LKAEIAELEAKLAPPDPSRFQKVEVVPAASSVDVLSIGIAWLC"
     gene            5637..6029
                     /locus_tag="PLB01_15640"
     CDS             5637..6029
                     /locus_tag="PLB01_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78786.1"
                     /translation="MPKISPRESVREHPLYRSAMAAQHAAYQLMRDVPASAKADATRL
                     HAALVHATTWATALLDDDVAEKDRTAARKGFEASVASASEMLGPLAPLADDAGIVDTL
                     EKRMEELRKVQIEAKSEPAAAPQAEPAA"
BASE COUNT          912 a         2198 c         2181 g          903 t
ORIGIN      
        1 ggacgggggc ttcgcggtgc ggctcgccga cgcgccggac gtccgggcgt cccgcgtgat
       61 cctcgcgacg ggcggccttt ccgtcctcgg cggcggcgcg gacggccgcg gctttgcgtg
      121 ggccgaggcg ctcggccaca cgctcgtgcc gacctatccc gcgctcgtcc cgctcacggg
      181 cgacgcgccc gcgcaccacg cgctctcggg cgtgagcgta aacgcgcgcg tcacggcgac
      241 gtgcgcggcg agcggcgaga aggccggttc ggaaggcggc ttcctcttca cgcaccgggg
      301 ctggagcggc cccgcggtgc tcgacgtctc gcacatcgtc gagcgggcgg cgcgcgcgga
      361 cgcgcgcgcg gaggtgaaca tctgcttcgg attcggagaa gaagagattt ctgcgaatgg
      421 tcccgtgcgc gaccgctggg acgagtctct gcgtgtgggc ggtggtgccg cctcctccct
      481 ctcttccttt ctaagaaaat cttcttctct tccggatcgc gtgatccaac tcgtccttca
      541 agaggctggc gtcgcggatc agccgctcgc gcggctgacg cgcgacgcgc ggcggcggct
      601 cgcgcagacg ctggccgcgt tcccgatccc cgcgacgggg acggagggct accgcaccgc
      661 ggaggtcacc ggcggcgggg tcgcactgga cgaggtcgat cccggcagcg gacggagcaa
      721 ggtcgtcccg gggctgtggc tcgcgggcga ggtgctggac gccttcggtc cgatcggcgg
      781 cttcaacttc cagtgggcgt ggggcacggg ctggacggcg ggcgtttcgg ccgcgcggcc
      841 gtgacctaga tcggcggctc gtcccaccat ttcgagaggc ggcctttcgg gaggccgggc
      901 gtcgacgtgc cggcgtgcgc gtaatgcgac acgtcgttga agagcgtgag ccgcgcctgc
      961 gccgggttct tgaagtcgag gacgttcagg cacgcggggc tctggtcgag gcggtcgcgg
     1021 tacgtgcgcg ggtcgaagcc gatgagcgcc gagaggagga ggcggatcgt cgccttgtgc
     1081 gagacgacga ggatcgagcg accggcgtgc tcgttcacga gcgagacgag tgcgggcagc
     1141 gcgcgcgccg tgacggcgag gcccgtctcg ccgcccttcg gcgcgaacgt gtacgggtcc
     1201 ctctcccagc aggcgtactc gtccggccac agggcctcga cctcggtgcg ggtccgggcc
     1261 tcccagcggc cgtgcgagat ctcgcgcaag gccggcaccg gaagaacgga cagcccgtgc
     1321 ggcgccgcga gaatcctcgc gctctcgagc gtgcgccgca tggggctcgc gcagacggcg
     1381 gcgagcggct cgcgcgcgag gcgcgcggcg aggtggtgca gctgttcgcg cccctcctcc
     1441 gagagctcga cctcggtttc gcccgcgaag cggtcctcgg ccgagagcgt cgtcgcgccg
     1501 tggcgaacca ggaacacgcg cgtcggcatt cgggtcagcc cttcacgagg agattcttag
     1561 aaggtaagag aatgcggagg cgttcgcgcc actcgccctg cagctcgatg gtcgcgccga
     1621 cgacggatcc acctgtgccg agggatttct tgaggtcctt cgctaagcct tccaagaaat
     1681 cttcattcct cggcagcccg tcgagaaccg tcaccgtctt gccgccgcgc cccttcttct
     1741 cgagccgcag cttcacgacg accttcgcag ggaccttctc ttcctctttc gaagagcatt
     1801 tgcattcgtc cgccggccag ccgcacgttg cgcagacgcg gccggacgcg gtggagtaga
     1861 cgaggcggac ggggtcgcgc cggatcaccg gaggattatg ctcggcgccg tggccgatcc
     1921 gatcagcgcg ccgcccggcg tgttcgtccc gcccgctgcc ctcgaggttc acgccgtgcg
     1981 ctcgggcggg ccgggcgggc agaacgtcaa caaggtcgcc tcgaagatcc tcctcgaagt
     2041 ggatctcgcg aagatcgagg gcctgagcga cctcgcgcgc cgccgcctcg cgaccctcgc
     2101 gggccgacgc ctcggcgcgg acggaatcct gcgcgtcacg gcgcaggaca gccgcgacca
     2161 gtaccgcaat ctcgccgcgg cgcgcgacaa ggtccgcgag ctcctcgagc aggcgcaggt
     2221 cgcgccgaag gcgcggcgcg cgacgcggcc gtcacgagcc gcgaaggaaa agcgactgaa
     2281 agccaagcgc gtggccgcga tccgaaaagc gcggcgcggc tcggtgcgcg acgaagactg
     2341 acctgaggag atcgacatga accgaaagct cgccttcgcc ctcttcgccg cggccctctt
     2401 cgccccggtg cccgcccgcg cgcaggcgaa ggcggacctc caggtcacgg cgttcgggtt
     2461 caccggtccg acggcgaata cggtcccgaa gccgtcctgc gagccgaaca cggtcgtcta
     2521 cagttttgcc gtggtcgtca cgaacgcggg cgccggaccg tcgccgtcat cggcttcgct
     2581 cgggggccgg ccgctcctga cggtcgcggc ccaggaccgc gccggctgga tggcgagcgt
     2641 tccgctcccg gaaatcgcgc ccgggaagtc cgtttccgtg aatgccgacg tcctcttcct
     2701 cgcttcggac ccggcctaca tggtcaaggc caaccacccg ttcctcgcga cggtcgaccc
     2761 cggcaacctc gtcgacgagg cggacgaggc gaacaacacg aagggtccgc tcacgatggg
     2821 cccgcccgcc ggctgcgagc gcttcttcaa gaagaagtga ggctcaggcc cacgcgccgg
     2881 aggccggctt ccggatcggc acgggcctgc cgtcctcgcc gacgttcacg tagaccacct
     2941 cggcctccgt caccttcagg accttgcccg actccttcca ccgctccgcg gtgacggtga
     3001 cgtgcacgct gatcgacgtc gtcccgatct tcacgacctt cgcatagaag ctcacgacgt
     3061 cgcccacgta caccgggtga tggaagacga cttcgttcat cttcacggtc accacgaggt
     3121 tgcagaacgg gcgcgtcgcg atcgcgccgg cctggtcgat gtaggcgaga aggacgccgc
     3181 cgaagatcgt cccgtgcccg ttcgtgtcgc ggggcagcat cgagacgcgg atcgcgggct
     3241 cgtgggcgtt cgtgtccatg ccgagacgat acgccggcgc taagatcgcg cgcatgcccg
     3301 acacgaagac cgcgctcttc ctgggccgcg ccgtggaccc ctcgaccgga aagaccggcc
     3361 gcgaggattt ccggctcgac ccctccgacc tcacgactca cggcctcatc gtgggcatga
     3421 cgggcagcgg caagacggcg ctgggcatcg tcctgatcga agagcttctc cagcgcggga
     3481 tcccggtcct cgccgtggat cccaagggcg acctcggcaa tctgctcctg aacttcccgg
     3541 gcctcgcacc ggaggagttc gcgccgttcg tcgacccgtc cgccgggacg ccggagtccg
     3601 aggcgaagaa gtggacggac ggactcgcgg gatggggcct cggccctgcg gacgtgaagg
     3661 cgctcctggc cagccgcgac gccgtcgtct acacgccggg ctcgacggcg ggcgtgcccc
     3721 tcgacctctt cggcgcgtgc acgccgccgc tcgcgggcgc ggacgaggag gacggccgcg
     3781 acctcgtggg ctcgtgggtg ggcggcctcc tcggccttgc gggccgcgat gccgaccccg
     3841 tgaagtcgcg cgacttcgtc tttctcgcgg cctgcgtgga cgcgctctgg gcggacggca
     3901 ggaccgccac gctcgagacg ctcctcgaca cggcctcctc gccgcctttc gcgaaggtcg
     3961 gcgcgctctc cctcgatgcg ttctacccgg cgaaggaccg gcaggcgctc gtcctggcgc
     4021 tgaacggact tctcgcgtcg ccgtccacgg cggccttccg gcagggcgac gcgctcgacg
     4081 tcgcggcgat gctcgccccg gcgaaggacg gcagggcgcg cctctcgatc gtctcgatcg
     4141 cgcacctcgg cgacgccgag cgcctcttcg tcgtcgcgac gctgctcgcg cgcgtgaagt
     4201 cgtggatgcg cacgcttccc ggcagcccgg cgctccgcgc gctcgtctac gtcgacgaga
     4261 tcttcggctt cttcccgccc actgccgagc cgccgacgaa gaggccgctc ctcacgctcc
     4321 tcaagcaggc gcgcgcgttc ggcgtgggcg tcgtcctcgc gtctcagaac cccgtggacc
     4381 tcgactaccg cggcctcgcg aactgcggct gctggtgggt cgggacgctg cagaccgagc
     4441 gcgaccgcaa ccggctctcc gagggcctcg tcgaggccgg cggcagggac gcgtcgaagc
     4501 tcctcgacag gacgcgcaag cgcgtctttc tgcttcacga cgtccaccgc aaggagcccg
     4561 cgctcgtcga gacgcgctgg gcgatgagct acctgcgggg cccgatgacg aagtcggacc
     4621 tcgcgaagct gcgcggttcc gcgcccgccc cttcgaagaa agcggaggcg gcccctgccg
     4681 ggtccgcacc cccgcccctt ccgcaggagt ggcccgcgcg gtgggtcgac aggcgcgggg
     4741 cggatctcgc gtcgcccgcg ctctacgtcc ggtacgccgt ccgctacaag acggcgaagg
     4801 gcacgtcgcc cgagacgcag ggcgtcaagc tgttcccgct gtcggcgccc acggccgccg
     4861 acgtcctcga aggcgaggcg acggacatgg cggcggacgg cctcctcgac aaagcgccgc
     4921 gcgccctgcg ctacccggac ctcccggcct gggtcgggcc ggccggcgtg aaggccgccg
     4981 agaaggccgt gcgctcgcgc ctccccgaca agctcgccac gacgctcctg cgcgacccgg
     5041 tcaccggcgc gctctcgctc cccggcgaga cgccggacgc gttcgcggcg cgcgccgcgt
     5101 cgggcgccga ggctcccgca gcgctccggg agaggctgga aaagaagaag cgggacctcg
     5161 cggcggcgga agccaccgag caggggaggt cgatggaaac ctacgcgtcg atcggcacgg
     5221 cggcgctcga cgtgctcggc ggcctgctcg gaaagaagaa gacgctccgt gtgagcaagg
     5281 tgggctccgt gctgtccaag aagcgcatgg aggggacggc cgaggcgaag gtcgagggcc
     5341 tgaaggccga gatcgcggag ctcgaggcca agctcgcccc gcccgacccg agccgtttcc
     5401 agaaagtcga ggtcgtgccg gcggcgagca gcgtcgacgt gctttcgatc gggattgcgt
     5461 ggctttgctg aggaggaaag aagagggaga aagaagaaga ttttcttgga aggtgaatag
     5521 ggcggcggga agcgcgggcc cttccagcgc ggcgccggag cgcccccagc ccctcttcac
     5581 tttcaaagaa atcctctctt ctccgaagcc cctttttctt tctctacaat ccctccatgc
     5641 cgaagatctc cccgcgggag tccgtccgcg agcatccgct ctaccgctcc gcgatggcgg
     5701 cgcagcacgc cgcctaccag ctcatgcgcg acgtgcccgc gtccgcgaaa gccgacgcga
     5761 cacgccttca cgccgcgctc gtccacgcga cgacgtgggc gacggcgctc ctcgacgacg
     5821 acgtcgccga gaaggaccgg acggccgcac gcaaggggtt cgaggcgtcc gtcgcgagcg
     5881 caagcgagat gctcggcccc ctcgcgccgc tcgcggacga cgcggggatc gtggacacgc
     5941 tcgagaagag aatggaagag cttagaaagg tgcagatcga ggcaaagtca gaacccgcgg
     6001 ccgcgccgca ggccgagccg gcggcttgaa cgtcgccagc cctcttcacc ttctaaggag
     6061 atccccggtc attcttcctt tttcgtcccg aggaccacga ggacgatcag gagcaggagg
     6121 acgatgaggc cgatgacgat cgtcgtcgtg cgcgaggagc cggccggcgg ctcgggcgcg
     6181 ggcgccgcgg cctt
//
LOCUS       DAOMFY010000241         6181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4245_length_6181_cov_5.861084, whole genome shotgun sequence.
ACCESSION   DAOMFY010000241 DAOMFY010000000
VERSION     DAOMFY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4245_length_6181_cov_5.861084"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLB01_15645"
     CDS             <1..210
                     /locus_tag="PLB01_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78787.1"
                     /translation="LTEAALSFLGLGAPPPEASWGRALAEARNLLPGAWWCVLPPAAA
                     LFLLVLSARRLGDAAASRDVQSPSP"
     gene            207..692
                     /gene="coaD"
                     /locus_tag="PLB01_15650"
     CDS             207..692
                     /gene="coaD"
                     /locus_tag="PLB01_15650"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQQ78788.1"
                     /translation="MKQTAVYPGTFDPMTNGHVDLIRRGCHIFDRVMVAVLINTDKSP
                     LFTVEERLRMIGQIFKREPKVVVKSFRGLLVDFMRKENAHIVVRGLRVMSDFEHEFQM
                     ALMNRRLGPQVETLFLTPKEELSYTSSRLVKQVHQLGGDVTGLVPPLVRRALLKKLPH
                     R"
     gene            complement(711..1154)
                     /locus_tag="PLB01_15655"
     CDS             complement(711..1154)
                     /locus_tag="PLB01_15655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQQ78789.1"
                     /translation="MPDEKQAEPKGPAVTCTLAVRYAETDAQRVVYHGNYIVWFEVGR
                     TTYCERVGYPYARMEAEGIFITVVDVRVRYRKSARYGDVVTIRTRCSEMKSRGCAFTY
                     DVLLPDGSLAAEGETRHLFLDAGGRPRTAPPGIADAFRAFMGPAA"
     gene            1174..2373
                     /locus_tag="PLB01_15660"
     CDS             1174..2373
                     /locus_tag="PLB01_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HQQ78790.1"
                     /translation="MSARVLFAGNGWGLGDGGEEKIFFEGDEGRARADGPEQLPEVLA
                     ALDAVAACGDPHLLAAGFISYEAGVWIEGSTALVRGHDFLPFAEFFIFNTRSSSVSRR
                     APPPPADFSSFTLEERASAERSLSSDEWARGVGAIRDGIARGDVYQVNLSHRTRFAAR
                     VDPFGLARELHAKSPVPYAVTLAAEDWAVVSNSPELFLDVDLRARSAVTKPIKGTAPR
                     GDSAESDAEAGRRLVASAKDRAENVMITDLLRNDLGRVAVPGGVATTALAGLRTFAHL
                     HHLESTVEARLAPGTRLSDVLRATLPGGSITGAPKRAALGFIRRLEPVARGAYTGAAG
                     YVRGDGRAVFNVAIRTAITGRDTVDYHAGGGIVWDSNAGAEWAETLTKSREMEAFLGA
                     RAEPARG"
     gene            2366..3154
                     /locus_tag="PLB01_15665"
     CDS             2366..3154
                     /locus_tag="PLB01_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQQ78791.1"
                     /translation="MAEFAPRPYHSPAVQRGFGFFETALLVGRRAVLWDAHLDRLLRG
                     LAHLDLPAPSREALDAAARAALDVRAPRGEEQSGLRLTWLAAGEDVDAAASWRLDCYV
                     LPIPESRLARRNGSHAVSLPLDLQRDTPGVKSTSYFAALQGGRLAKKAGADEGLFRGH
                     DGRYLEGTTTGLAAWNGGTPVLAPEGVLPSVTAAAFFDGGAALAPIGEEHLRAGAVLL
                     GSLTTAAPLLSLDGRPCAQPTEMLARIRAFRERLISDPSLSRAL"
     gene            3176..4303
                     /locus_tag="PLB01_15670"
     CDS             3176..4303
                     /locus_tag="PLB01_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ78792.1"
                     /translation="MATRKVRKPASERSTGGTILFTGFPGFIGARLIPRLLELRPGAF
                     FECLVQEKFLPAADASVAALAAAHPHTKGRIRLVTGDITAPGLGLGAGAAKALAKKVT
                     GCFHLAAVYDLAVARDVAMRINVEGTKNVLEFVGGAPGFRRLDYVSTAYVSGTAVGSY
                     REKDLDVGQSFKNHYEETKFLAEVAVKESGLPAAIYRPGIVVGDSRTGETAKFDGPYF
                     ALNAMSLLPSPGAFIRIGDGAAEVNLVPIDYVLEAIAQLGSWDGAIGKTYHLTDPAPE
                     TALGVAELLAKSIGKSFVFVPVPLPIAKLAFVVPQVSGWLGMPGQTLDYFDHPVHYDS
                     AQATKDLARFGLECPPFRAYVQRLVAFWKKKRAEITRGAMI"
     gene            4314..5960
                     /locus_tag="PLB01_15675"
     CDS             4314..5960
                     /locus_tag="PLB01_15675"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQQ78793.1"
                     /translation="MKKRAFTNKPIIDGQDVDTGKTFKAVNPATGEPFAEVFRAGPAE
                     IDAAVASARKAFLSWSKTPVTARQKLLARLLEEVRKSHEDIARLIATEQGKPVGEARA
                     VDVVPAADTLRYLSRHAASILAERPVDYAQILFAHKSGSYRLEPLGVVAIVTPWNFPF
                     GIPFVEVAACLAAGDTVVLKPASATALTGLAVGDLCRRAGIPPGVVNVVTVGGEDTNR
                     LIEHPGIAKILFTGAVETGVGVMQRAAKNLTGVVLELGGKDPAVVAADADLDRASSGL
                     VWGAFVNAGQACGSVERVYVVREVAEEFLGKVVAKAKALRLGDPLSPSTDMGPLTTEA
                     QRALVEAHVADAKAKGARILTGGKRPRAKGWWYPPTVLANVDHSMRCMHEETFGPLLP
                     VMVVDSLDEGIRLANESEFGLTASGWTRSRKTAQRLVEELHAGTVTINDHLFSFGEPT
                     ATWGGVKKSGVGRSHAAFGLHELVNIKHVSVDMGNAGAMPWWYPYDSAFHAFTKKAFG
                     TLYSNDPRTKVPDALGLLGSGRFFGYVKVSTLASRLGKVF"
BASE COUNT          879 a         2197 c         2182 g          923 t
ORIGIN      
        1 ctcacggagg ccgcgctctc gttcctcggc ctcggcgcgc cgccgccgga ggcctcctgg
       61 ggccgggcgc tcgccgaggc gcgaaacctg ctgccgggcg cgtggtggtg cgtcctgccg
      121 cccgcggccg cgctcttcct gctcgtcctc tcggcgcgcc ggctgggaga cgccgcggcg
      181 tcccgggacg tacaatcgcc gtccccgtga agcaaaccgc cgtctacccg ggcacgttcg
      241 accccatgac gaacggccac gtcgacctca tccggcgggg ctgtcacatc ttcgaccgcg
      301 tcatggtcgc cgtcctcatc aacacggaca agtccccgct cttcacggtc gaggagcgcc
      361 tccggatgat cggccagatc ttcaagcgcg agccgaaggt cgtcgtgaag tcgttccggg
      421 gcctcctggt ggatttcatg cgcaaggaga acgcgcacat cgtcgtgcgg ggcctgcgcg
      481 tgatgtcgga cttcgagcac gagttccaga tggcgctcat gaaccgccgc ctcggaccgc
      541 aggtcgagac cctgttcctg acgccgaagg aagagctctc gtacacgtcg agccggctcg
      601 tgaagcaggt ccaccagctc gggggcgacg tcacgggcct cgtgccgccg ctcgtgcggc
      661 gcgccttgct caagaagctc ccgcaccgct gagacgcgcg cgcggcctgc ctaggccgcc
      721 gggcccatga acgcgcggaa cgcgtccgcg atgccgggcg gggccgtccg cggacggccg
      781 ccggcgtcga ggaacaggtg gcgcgtctcg ccctccgcgg cgaggctgcc gtcgggaagg
      841 agcacgtcgt aggtgaacgc acagccgcgg ctcttcatct ccgagcaccg tgtccggatg
      901 gtcacgacgt cgccgtaccg ggcggacttc cggtaccgca cgcgcacgtc gacgaccgtg
      961 atgaagatgc cctcggcctc catccgcgcg tacgggtacc cgacgcgctc gcagtacgtc
     1021 gtgcgcccga cctcgaacca gacgatgtag ttgccgtgat agacgacgcg ctgcgcgtcg
     1081 gtctcggcgt agcgcacggc gagcgtgcaa gtgaccgcgg gacccttcgg ctcggcctgc
     1141 ttctcgtcgg gcatgtcgcg cagaatagcc gacgtgtccg cacgcgtgct gttcgccgga
     1201 aacggatggg gattggggga cggcggagaa gagaagatct tctttgaagg tgacgagggg
     1261 agggcgcgcg ctgacgggcc ggagcagctc cctgaagtcc tcgccgcgct cgacgccgtg
     1321 gcggcctgcg gcgacccgca cctcctggcg gccggattca tctcctatga ggcgggtgtg
     1381 tggatcgaag gctcgaccgc gctcgtgaga gggcacgatt ttcttccctt cgcagaattc
     1441 ttcatcttca atacccgcag ttcttccgtc tctcgccgag ccccgccgcc tcccgctgac
     1501 ttttcctctt tcacactcga agaacgggcg agcgcagagc gctcgctctc ctcggacgag
     1561 tgggcgcgcg gtgtcggcgc catccgggac gggatcgcgc gaggcgacgt ctaccaggtc
     1621 aatctctccc atcgcacgcg gttcgccgca cgcgtcgacc cgttcgggct ggcccgggag
     1681 ctccacgcga agagcccggt gccgtacgcc gtgaccctcg cggcggagga ttgggccgtc
     1741 gtctcgaact cgccggagct gttcctcgac gtcgaccttc gcgcccggag tgccgtcacg
     1801 aagccgatca aggggacggc gccgcggggc gattcggccg agtccgacgc ggaagccggc
     1861 aggcgcctcg tcgcgtcggc gaaggaccgc gccgagaacg tcatgatcac ggatctgctg
     1921 cggaacgacc tcgggcgcgt cgcggtcccg ggaggcgtcg cgacgacggc tctggccggc
     1981 ctgcgcacgt tcgcgcacct ccatcacctc gagtcgaccg tcgaggcgcg tctcgccccc
     2041 ggcacgcggc tctccgacgt tctccgggcg acgctgcccg ggggttcgat cacgggcgcc
     2101 ccgaagcggg ccgccctcgg cttcatccgg cgcctcgaac cggtcgcacg gggcgcgtac
     2161 acgggcgccg ccgggtacgt ccgcggcgac ggccgggcgg tcttcaacgt ggcgatccga
     2221 acggcgatca ccggccggga tacagtcgac tatcacgcgg ggggtggaat cgtgtgggac
     2281 tcgaatgccg gcgcggagtg ggccgagacg ctcacgaagt cgcgggagat ggaagcgttc
     2341 ctcggcgcgc gcgcggagcc ggcccgtggc tgagttcgcg cctcgcccgt atcactcgcc
     2401 ggcggtccag aggggcttcg ggttcttcga gacggcactt ctcgtcggcc gcagggcggt
     2461 gctgtgggac gcccacctcg accggctcct caggggcctc gcgcacctcg acctgccggc
     2521 gccttcgcgc gaagcgctcg acgccgccgc ccgcgccgcc ctcgacgtcc gcgcgccccg
     2581 cggcgaggag cagtccggcc tgcgcctcac gtggctcgcg gccggggagg acgtcgacgc
     2641 cgcggcttcg tggaggctgg actgttacgt gctcccgatt cccgagagcc gcctcgcgcg
     2701 ccgcaacggc tcgcacgcgg tgtcgctgcc cctcgacctg cagcgcgaca cgcctggcgt
     2761 gaagtcgacg agctacttcg cggcgctcca gggcggccgc ctcgcgaaga aggcgggcgc
     2821 cgacgagggc ctcttccgag gccacgacgg ccgttacctc gaagggacca ccaccggact
     2881 cgcggcctgg aacgggggaa cgcccgtcct cgcgcccgag ggcgtcctgc cgagcgtcac
     2941 cgcggccgcg ttcttcgacg gaggggcggc ccttgctccg atcggcgagg agcacctccg
     3001 cgccggcgcc gtcctgctcg gctcgctcac gacggcggcg ccccttctgt cgctggacgg
     3061 gaggccctgc gcgcagccga cggagatgct cgcgcggatc cgggccttcc gggagaggct
     3121 gatctccgac ccgtccctct cacgggctct ctgaggggac tatattgacc tcctaatggc
     3181 gacgcgcaag gtccggaaac ccgcctccga acgctccacc ggcgggacca tcctcttcac
     3241 cggcttcccg ggcttcatcg gagcccggct cattccgcgc ctcctcgagc tccggcccgg
     3301 ggctttcttc gagtgcctcg tccaggagaa gttcctgccc gcggccgacg cctcggtggc
     3361 agccctcgcg gccgcccacc cgcacaccaa gggccggatc cggctcgtga cgggcgacat
     3421 cacggcgccg ggcctcggcc tcggcgcggg cgccgcgaag gccctcgcga aaaaggtgac
     3481 gggctgcttc cacctcgccg ccgtgtacga cctcgcggtg gcccgcgacg tggcgatgcg
     3541 catcaacgtc gagggcacga agaacgtgct cgagttcgtg gggggcgctc cgggctttcg
     3601 gcgcctcgac tacgtgtcga ccgcgtacgt ttcggggacg gcggtcgggt cgtaccgcga
     3661 gaaggacctc gacgtcggcc agtccttcaa gaaccattac gaggaaacga agttcctcgc
     3721 cgaggtcgcc gtgaaggaga gcggcctgcc cgccgcgatc taccgtcccg gcatcgtcgt
     3781 cggggactcg aggacgggag agacggcgaa gttcgacggt ccctacttcg cgctcaacgc
     3841 gatgagtctc ctcccgtcgc ccggcgcgtt catcaggatc ggggacggcg cggccgaggt
     3901 gaacctcgtg ccgatcgact acgtcctcga ggcgatcgcg cagctcggct cgtgggacgg
     3961 ggcgatcggg aagacgtacc acctgacgga ccccgcgccc gagaccgccc tcggtgtggc
     4021 cgagctcctc gcgaagtcga tcgggaagtc cttcgtgttc gtgcccgtcc cgctcccgat
     4081 cgcaaagctc gcgttcgtcg tgccgcaggt ctcgggctgg ctcggaatgc ccggccagac
     4141 gctcgactac ttcgaccatc cggtccacta cgactcggcg caggcgacga aggacctcgc
     4201 acgcttcggg ctcgagtgcc ctccgttccg cgcgtacgtc cagcggctcg tggcgttctg
     4261 gaagaagaaa cgcgccgaga tcacgcgcgg ggcgatgatc tgagggcgcg ccggtgaaga
     4321 agcgcgcgtt cacgaacaaa cccatcatcg acggacagga cgtcgacacg gggaagacct
     4381 tcaaggccgt gaatcccgcc acgggagagc cgttcgcgga agtcttccgc gcgggtccgg
     4441 ccgagatcga cgccgcggtc gcgtccgccc gaaaggcgtt cctgtcgtgg tcgaagacgc
     4501 cggtgaccgc gaggcagaag ctccttgcgc gcctcctcga ggaggtccgc aagtcgcacg
     4561 aggacatcgc gcgcctcatc gcgacggagc agggcaagcc cgtgggcgag gcgcgcgccg
     4621 tggacgtcgt cccggccgcg gacacgctga ggtatctctc gcggcatgcc gcttcgatcc
     4681 tcgccgagcg cccggtcgac tacgcgcaga tcctcttcgc gcacaagtcg ggctcctacc
     4741 ggctcgagcc gctcggcgtc gtcgcgatcg tcacgccgtg gaacttcccg ttcgggatcc
     4801 cgttcgtcga ggtcgccgcc tgcctcgcgg ccggggacac cgtcgtcctc aagccggcct
     4861 cggcgaccgc gctcacgggc ctcgccgtcg gcgacctctg ccggcgcgcc ggcatcccgc
     4921 cgggggtcgt gaacgtcgtg accgtgggcg gcgaggacac gaaccgcctc atcgagcatc
     4981 cggggatcgc gaagatcctc ttcacgggcg ccgtcgagac gggcgtcggc gtcatgcagc
     5041 gcgcggcgaa gaacctcacg ggcgtcgtcc tcgagctggg cggcaaggac ccggcggtcg
     5101 tcgcggccga cgccgacctc gacagggcct cgtccggcct cgtctggggc gcgttcgtga
     5161 acgccggcca ggcctgcggc tccgtggagc gcgtctacgt cgtgcgggag gtggccgagg
     5221 aattcctcgg gaaggtcgtc gccaaggcga aggcgctgag gctcggcgac ccgctctcgc
     5281 cgtccacgga catgggcccc ctgacgacgg aggcgcagcg cgcgctcgtc gaggcgcacg
     5341 tcgccgacgc gaaggcgaag ggtgcgagga tcctcacggg ggggaagcgc ccgcgcgcga
     5401 agggctggtg gtacccgccg acggtcctcg cgaacgtcga ccactcgatg cgctgcatgc
     5461 acgaggagac gttcggtccg ctcctgccgg tcatggtcgt cgactcgctg gacgagggaa
     5521 tccgcctcgc gaacgagtcg gagttcggcc tcacggcctc gggctggacg cgctcgcgca
     5581 agacggcgca gcggctcgtc gaggagctcc acgcgggcac cgtgaccatc aacgaccacc
     5641 tgttctcgtt cggcgagccg acggcgacgt ggggcggcgt caagaagtcg ggcgtgggac
     5701 gcagccacgc cgccttcggc ctgcacgagc tcgtgaacat caagcacgtg agcgtggaca
     5761 tggggaacgc gggtgcgatg ccgtggtggt acccgtacga ctcggccttc cacgcgttca
     5821 cgaagaaggc gttcgggacc ctctactcga acgacccgcg gacgaaggtg ccggatgccc
     5881 tcggactgct cggcagcggg cgcttcttcg gctacgtcaa ggtctcgacg ctcgcgtcgc
     5941 ggctgggcaa ggtcttctga aacaagaaag cggccccccc ttccgggagg gccgcttcgt
     6001 gaccggagct tctctgaact agctctggcg gcggatcgcg cggaggcccg cgccagccgc
     6061 gacggacagg ccgccgagga ggccgacgag cggcatcggg ctcgccgtct tcgggagctt
     6121 cttcttggcg ggcttggcct cggcgacggg ggccggagcc ggggccgggg ccggagccgg
     6181 g
//
LOCUS       DAOMFY010000242         6171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4259_length_6171_cov_5.158764, whole genome shotgun sequence.
ACCESSION   DAOMFY010000242 DAOMFY010000000
VERSION     DAOMFY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_6171_cov_5.158764"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PLB01_15680"
     CDS             complement(<1..1642)
                     /locus_tag="PLB01_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQQ78794.1"
                     /translation="MPSARFSGLPLLKAFEGALKEGLLLDEALGGILNAALHFFDAPV
                     VALLPGAGVPPMTRSGRSSVAAAAETRLNQHLAEVLAQGRAKKANEGGLSFFGAPVKV
                     KDQTQAVFGVVLETIAGTDAEAEEAAREFARTIGHVLERDRTLGTLMKRREEAVALFE
                     LASGAFLSLNPDEVIRLTVASLSRELEFDRVTAYRFLPETKEVAEILTHGAPTSGERP
                     VSGRTPIASDELLARCLAAHGPAFEDEPGTQPVRRRRMALPLHAGAHVFGFLAVSRRG
                     GFALTPQELRLSQELAKLAAGALEKARLLDGERRNNDRVAFVGRLHAALTGLTDVPSI
                     MSRTVAELGPQFDLDLCAVKLLPSGDLPGAEAVYVKAGGTTELQIPDALHAALSTEGS
                     HAFYGNASADKHGQALLPSGPAIAALAKPLGLLAVPLAFRGTPVGILCAIAAAPQSLS
                     LDLLPSFEALGVEVSLAVASVRLLQQERESYRFLDRLREVGRSLSTTFDVERIKQTLC
                     EQAVSLLKADAAQFWDADPASKSVKIGARWGADVGDELA"
     gene            1683..3194
                     /locus_tag="PLB01_15685"
     CDS             1683..3194
                     /locus_tag="PLB01_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQQ78795.1"
                     /translation="MPRANANPGALEAPAAATESASTGALAESLAEILPALERFTRYE
                     GPDGAAKRTAWRRALDGPLPEDGEGREAVLAALRDVVIPNGLRIGAPGFTGWVTTSPT
                     TVPAVAQLAGSITAAQRWWATPGNFLEVLALRWVGRLVGLPDTFEGSFTSGGATANLL
                     GLAAARQHAGERLGVDAGLEGLAALPSPRVYASTEVHHVVHRACGVLGLGRKALRLVP
                     MDGARRVDLAALERLLDEDVRAGATPVAVVASAGDVNTGVVEPVDAMREIAHARGVWL
                     HVDGAYGGFGVLDPRVAPLYGDLGKIDSFAVDPHKWMAAPVGCGAAFVRDGGLLARAL
                     ALEPAAYVEAAPTDTADTGSPFDQMGEGDPNYSLDHSAPPRGLAVWALLKEIGAKGMR
                     ERVVRHHDCARRVAERVRAHPALELLAEPVLSICCFRVRPAGVTDEAALEALNEKVLL
                     AVRAKGRAIPSHTRVDGRFALRPCFINPRQGLADADATVDEVLAAAQALGAIP"
     gene            complement(3199..4422)
                     /locus_tag="PLB01_15690"
     CDS             complement(3199..4422)
                     /locus_tag="PLB01_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQQ78796.1"
                     /translation="MPSSHRSRAIQLFLSTALVPALLFPAAARADDEPCAPTFRIASG
                     IGPANAVVLGAWDLDRDGRTDLLVRTAAAEVRVAHNSGGGAFVLGDFLTYGRVVVGDF
                     DRDGILDVVAVTTSGSSSVVAFLRGDGRAVFRQVSTVSVAAALTAPVAADFDGDGALD
                     LAASAGSDVWWFRGDGHGGFAAPSVLHTSASTVSRLYALDVDADGRPELLAAGTGGFA
                     YDTLATYTVRSGAFVEVAGPEAGGFQCPFDVALGDVDGDGHADAVTASRYIGGCPDGI
                     RTFLARSGGPVLLRQATPSLWSPVVADFDGDGHADLAAIQGSLYGAPTGLVVLLGDGA
                     GAFGPATFFTLPGNVNRETALLTAADLDGDRRIDLVLSGVPGLPPLFLRNQCGNPHEP
                     ATPGPALPAQRRVRP"
     gene            complement(4454..4930)
                     /locus_tag="PLB01_15695"
     CDS             complement(4454..4930)
                     /locus_tag="PLB01_15695"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HQQ78797.1"
                     /translation="MTLSDRLASPWLTVRVQIALGVFFVAAALPKVVDPPAFAHMIYN
                     YRIVPGAFLNVMALVMPWFELLAGIALILGIWTRTSAALIGALLLVFIAAISFNLARG
                     NAIDCGCFDVASAGKSVAERIADMRWVVIRDVGMLVMVAQILWATGRMEDENPSLR"
     gene            complement(4927..5703)
                     /locus_tag="PLB01_15700"
     CDS             complement(4927..5703)
                     /locus_tag="PLB01_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQQ78798.1"
                     /translation="MRFKRHIGEALTLVAAAVLCALVANALARAERKLPLVGTYPNAL
                     KVPELKTAAASSPADSGAPATSGISSKVNGGGAPAPDAPATVATPQATPAAPAAAKSP
                     FEEKVPGPRAKSAGAAPVPTPAAEQRESLLSRFPPHPDKPYVEISGDDAAWLVARGVL
                     VIDARRKKDYEQGHVAFARNISPWEGDADAKITALVNEGRDGAQPVVVYCSGGDCEDS
                     HMLATRVYGGGFNNLLVYKDGWPDWVKRGGKSATGPEPAP"
     gene            5778..>6171
                     /locus_tag="PLB01_15705"
     CDS             5778..>6171
                     /locus_tag="PLB01_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQQ78799.1"
                     /translation="MAEKNSISITDNRTGKSYELPVENGTIRALDLRKIKTGDDDFGL
                     MTYDPAFTNTASCKSGITYIDGDKGILEYRGYPIEQLAQKSTFLEVAYLLLKGELPTA
                     SELKEFTHHVSIHTYLHENLKKLMEGFRH"
BASE COUNT          885 a         2149 c         2362 g          775 t
ORIGIN      
        1 gcgcgagctc gtcgccgacg tcggcgcccc agcgcgcgcc gatcttcacg gacttggacg
       61 cggggtccgc gtcccagaac tgcgccgcgt ccgccttgag taggctcacg gcctgttcgc
      121 agagcgtctg cttgatgcgc tcgacgtcga acgtcgtcga gagcgagcgg ccgacctcgc
      181 gcagccggtc gaggaaccgg taggactcgc gctcctgctg gaggagtcgg acggacgcga
      241 ccgcgaggga cacctcgacg ccgagggcct cgaaggaggg gaggaggtcg agggagaggc
      301 tctgcggagc cgccgcgatc gcgcagagga tgccgacggg cgtgccgcgg aacgcgagcg
      361 ggaccgcgag aaggccgagc ggcttggcga gggcggcgat cgcgggcccg gacggcagga
      421 gcgcctggcc gtgcttgtcg gccgacgcgt tgccgtagaa cgcgtgcgag ccctcggtcg
      481 agagggcggc gtggagagcg tcggggatct gcagctccgt ggtgcccccg gccttcacgt
      541 acacggcctc ggcgccgggc aggtcgcccg acgggagcag cttcacggcg cacaggtcga
      601 ggtcgaactg cggcccgagc tcggcgaccg tgcggctcat gatcgagggc acgtccgtga
      661 ggcccgtgag cgcggcgtgc aggcggccga cgaaggcgac gcggtcgttg ttgcggcgct
      721 cgccgtcgag gaggcgggcc ttctcgagcg cgcccgcggc gagcttcgcg agctcctgcg
      781 agaggcgcag ctcctgcggc gtgagcgcga agccgccgcg ccgcgagacc gcgaggaatc
      841 cgaacacgtg cgcgcccgcg tgcaacggga gcgccatccg gcgcctccgc acgggctgcg
      901 tgccgggctc gtcctcgaac gcgggcccgt gcgcggcgag gcagcgcgcg aggagctcgt
      961 cggaggcgat gggcgtccgg ccggacacgg gccgctcgcc gctcgtgggg gccccgtgcg
     1021 tcaggatctc ggcgacctcc ttcgtctcgg gcaggaagcg gtacgccgtg acgcggtcga
     1081 actcgagctc gcgcgagagg gacgcgaccg tgagccggat gacctcgtcg gggttcaggg
     1141 agaggaacgc gccggaggcg agctcgaaga gcgccacggc ctcctcgcgc cgcttcatga
     1201 gcgtgccgag cgtgcggtcg cgctcgagga cgtggccgat cgtccgcgcg aactcccgcg
     1261 cggcctcctc ggcttcggcg tccgtgcccg cgatcgtttc gaggacgacg ccgaacacgg
     1321 cctgcgtctg gtccttgacc ttcacggggg cgccgaagaa ggagagcccg ccctcgttcg
     1381 ccttcttggc gcggccctgg gcgaggacct cggcgaggtg ctggttcagg cgcgtctcgg
     1441 ccgcggcggc gacgctcgaa cggcccgagc gcgtcatggg cggcaccccg gcgccgggca
     1501 ggagcgcgac gacgggggcg tcgaagaagt gcagcgcggc gttgaggatg ccgccgagcg
     1561 cctcgtcgag gagcaggccc tccttgagcg ctccttcgaa cgccttcagg agtgggaggc
     1621 cggagaagcg ggcggacggc atggcggcga acggccataa taccgcctcc ccggaggtgc
     1681 ccatgccccg cgcaaacgcg aatcccggcg ctctcgaggc gcccgccgcc gcgaccgagt
     1741 ccgcgtccac gggcgcgctg gccgaatcgc tcgcggaaat cctgcccgcg ctcgagcgct
     1801 tcacgcgtta cgaggggccg gacggcgccg cgaagcggac cgcgtggcgc cgcgcgctgg
     1861 acggcccgct gcccgaggac ggcgaggggc gcgaggcggt cctcgcggcc ctgagggacg
     1921 tcgtcatccc gaacggcctg aggatcgggg cgccgggctt cacgggctgg gtcacgacgt
     1981 cgccgacgac cgtccccgcc gtcgcgcagc tcgcgggctc gatcaccgcg gcccagcgct
     2041 ggtgggcgac gccgggcaac ttcctcgagg tcctcgcgct ccggtgggtc gggcggctcg
     2101 tcggcctccc ggacacgttc gagggctcgt tcacgagcgg cggcgcgacc gcgaacctcc
     2161 tcggcctcgc cgcggcgcgc cagcacgcgg gagagcgcct cggcgtggac gccggcctcg
     2221 aggggctcgc ggcgctcccg tccccgcgcg tctacgcctc gacggaggtc caccacgtcg
     2281 tccaccgggc ctgcggcgtc ctcggtctcg gccgcaaggc gctgcgcctc gtcccgatgg
     2341 acggggcccg gcgcgtcgac ctcgccgccc tcgagcgcct cctcgacgag gacgtccgcg
     2401 cgggcgcgac gcccgtcgcg gtcgtcgcct ccgcggggga cgtgaacacg ggcgtcgtcg
     2461 agcccgtcga cgcgatgcgc gagatcgcgc acgcgcgcgg cgtctggctg cacgtggacg
     2521 gggcgtacgg cggcttcggc gtcctcgacc cgcgcgtcgc cccgctctac ggcgacctcg
     2581 ggaagatcga ctccttcgcc gtcgatccgc acaagtggat ggcggcgccg gtgggctgcg
     2641 gggcggcctt cgtgcgcgac ggcgggctcc tcgcgcgcgc cctcgccctc gagcccgcgg
     2701 cgtacgtcga ggcggccccg accgacacgg cggacacggg ctcgcccttc gaccagatgg
     2761 gcgaggggga cccgaactac tcgctcgacc actccgcgcc gccgcgcggc ctcgccgtgt
     2821 gggcgctcct gaaggagatc ggcgcgaagg ggatgcgcga gcgcgtcgtg cggcaccacg
     2881 actgcgcgcg gcgcgtcgcc gagcgcgtgc gggcccaccc cgcgctcgag ctcctcgccg
     2941 agcccgtcct ctcgatctgc tgcttccgcg tcaggcccgc gggcgtgacg gacgaggccg
     3001 cgctcgaggc gctgaacgag aaggtcctcc tcgccgtgcg ggcgaagggg cgcgcgatcc
     3061 cctcccacac gcgcgtggac gggcggttcg cgctgcgccc ctgcttcatc aacccgcggc
     3121 aggggctcgc ggacgcggac gccacggtgg acgaggtgct cgcggcggcg caggcgctcg
     3181 gcgcgattcc ctgacgcgct acggccggac gcgccgctgc gcgggcagcg ccgggccggg
     3241 ggtcgcgggc tcgtgcggat ttccgcactg gttgcggagg aagagcggcg gcaggccagg
     3301 gacgcccgag aggacgaggt cgatccgcct gtctccgtcg aggtccgccg cggtgaggag
     3361 ggcggtctcg cggttcacgt tccccggcag cgtgaagaac gtcgccggtc cgaaagctcc
     3421 ggcgccgtcg ccgagcagga cgacgaggcc cgtcggggcg ccgtagaggg agccctggat
     3481 cgccgcgagg tcggcgtggc cgtcgccgtc gaagtcggcg acgaccgggc tccagaggga
     3541 cggcgtggcc tgccggagca ggacggggcc gccggagcgc gcgaggaacg tccggatgcc
     3601 gtccggacag ccgccgatgt agcggctcgc cgtgacggcg tcggcgtgcc cgtcgccgtc
     3661 cacgtcgccg agggcgacgt cgaaggggca ctggaagccg ccggcttcgg ggccggcgac
     3721 ctcgacgaac gcgcccgagc gcacggtgta cgtcgcgagc gtgtcgtacg cgaacccgcc
     3781 cgtgccggcc gcgaggagct cgggccggcc gtccgcgtcg acgtcgagag cgtagaggcg
     3841 cgacaccgtg gacgcggacg tgtggaggac ggagggcgcg gcgaagccgc cgtgcccgtc
     3901 gccgcggaac caccagacgt cggacccggc ggacgccgcg agatcgaggg cgccgtcgcc
     3961 gtcgaagtcc gccgcgacgg gcgccgtgag cgcggcggcg acggagacgg tggagacctg
     4021 gcggaagacg gcgcggccgt cgccccggag gaacgcgacg acggacgagg agccgctcgt
     4081 cgtcacggcg acgacgtcga ggatcccgtc gcggtcgaag tcgcccacga cgacgcggcc
     4141 gtacgtcagg aagtcgccga ggacgaacgc gccgccgccg gagttgtgcg cgacgcgcac
     4201 ctcggcggcc gccgtgcgga cgaggaggtc cgtcctgccg tcgcggtcga ggtcccacgc
     4261 gccgaggacg accgcgttcg cggggccgat gccggacgcg atccggaagg tgggcgcgca
     4321 gggctcgtcg tcggcccgcg ccgcggcggg gaagaggagg gccgggacga gagcggtgga
     4381 gaggaagagc tggatcgccc gggagcgatg agaagagggc atcttctctg gaaatgtccc
     4441 cgcgcggagg cggctaacgc agagacgggt tctcgtcttc catccgcccc gtcgcccaca
     4501 ggatctgcgc gaccatgacg agcatcccga cgtcgcggat gacgacccag cgcatgtccg
     4561 cgatgcgctc cgcgacgctc ttgccggccg acgcgacgtc gaagcagccg cagtcgatcg
     4621 cgttgccgcg cgcgaggttg aacgagatcg ccgcgatgaa gacgaggagg agcgcgccga
     4681 tgagcgccgc ggacgtgcgc gtccagatcc cgaggatgag cgcgattccc gccaggagct
     4741 cgaaccacgg catgacgagc gccatgacgt tcaggaacgc gcccggcacg atgcggtagt
     4801 tgtagatcat gtgggcgaac gccggcgggt cgacgacctt cggcagcgcc gccgcgacga
     4861 agaagacgcc gagcgcgatc tggacgcgca cggtgagcca gggggaggcg agccggtccg
     4921 agagcgtcac ggcgccggct ccgggcccgt cgcgctcttt ccgccgcgct tcacccagtc
     4981 cggccatccg tccttgtaga cgaggaggtt gttgaacccg cctccgtaaa cgcgcgtcgc
     5041 gagcatgtgc gagtcctcgc aatcgccgcc cgaacagtag acgacgaccg gctgcgcgcc
     5101 gtcgcgcccc tcgttcacga gcgccgtgat cttcgcgtcc gcgtcgccct cccagggcga
     5161 gatgttccgc gcgaacgcga cgtggccctg ctcgtagtcc ttcttgcgcc gcgcgtcgat
     5221 gacgaggacg ccgcgcgcga cgagccaggc cgcgtcgtcg ccggagatct cgacgtaggg
     5281 cttgtccggg tggggcggga agcgggagag aagagactcc ctctgctccg cggccggcgt
     5341 gggcacagga gccgcgcctg cggacttcgc gcgagggcca gggacctttt cttcgaatgg
     5401 tgatttggcc gcggcaggag cggccggcgt ggcctgcgga gtggcgaccg tcgcgggggc
     5461 gtccggcgcg ggcgctccgc ccccgttcac cttcgaagaa atcccggacg tcgccggtgc
     5521 tccggagtcc gccggcgacg aggccgccgc cgtcttcaac tccggcacct tcaaggcgtt
     5581 gggataggtc ccgacgagcg gcagtttgcg ctcggcgcgg gcgagggcgt tcgcgacgag
     5641 ggcgcagagg acggcggcgg cgacgagggt cagggcctcg ccaatgtgac gcttgaatct
     5701 cacgtttgca tcatatgcaa gccgatggcc gggaatagaa tgttcgatgg tccgcaagcg
     5761 gactccggaa ggaacgtatg gctgaaaaga actcgatttc gatcaccgac aaccgcaccg
     5821 gaaagtccta cgagctgcct gtcgagaacg ggaccattcg cgcgctcgac ctgaggaaga
     5881 tcaagacggg cgacgacgac ttcggcctga tgacgtacga ccccgcgttc acgaacacgg
     5941 cgtcgtgcaa gtcgggcatc acgtacatcg acggcgacaa gggcatcctc gagtaccgcg
     6001 ggtacccgat cgagcagctc gcgcagaagt cgacgttcct cgaggtcgcc tatctcctcc
     6061 tgaagggcga gctcccgacg gcctccgagc tcaaggagtt cacgcaccac gtctccatcc
     6121 acacgtacct ccacgagaac ctcaagaagc tcatggaggg cttccggcac g
//
LOCUS       DAOMFY010000243         6164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4266_length_6164_cov_8.264528, whole genome shotgun sequence.
ACCESSION   DAOMFY010000243 DAOMFY010000000
VERSION     DAOMFY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4266_length_6164_cov_8.264528"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLB01_15710"
     CDS             complement(<1..1206)
                     /locus_tag="PLB01_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78800.1"
                     /translation="MRGDGGFGRDSGTPKRRMLPAVTDVVRELARTVSADPAVLFKAA
                     RSVVADELGKVKQGFESAPLDVLVRRARRQLEQEGVAAPAAEEPDEPRSVSIPAPPLK
                     PRKPASPPADDPFQGAGSADLEWEKDLNIQPDDAPFRSAILPLPPRSRVRGIAQPEST
                     VVPASAPPSPPRLPTPDRVVAPEGFAMERSGAASRPKAPVDDLPLFSSPTLELSSPLA
                     PAPPPRATEPAPPEAPALSEFPAFTPPEPPSPPSPPRVEEPDFARPDPWALPQDEPAF
                     EAASEPLGAAAPRFEPPAPPVEIPAPWVEPPVRRIDPPVKSLSEMPPLTNLVGDVTEP
                     VEPAMEEFAFKAAEGAPAKKSGMTGWIVAIAVVLVVAGGLTWAVRTFLSGDVVKKVEA
                     PAPAPKKVEA"
     gene            complement(1203..2222)
                     /locus_tag="PLB01_15715"
     CDS             complement(1203..2222)
                     /locus_tag="PLB01_15715"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQQ78801.1"
                     /translation="MTSKKDVLHVGIDLGTSRSAIAASNGTKHVVESYVGWPLDMVAR
                     KIVKKTILFGAEALDARSMLDLHRPLERGLIKQGSAKDEEAVHELLRHLIALAGRVEG
                     QRVEAVVGVPAEALRVSRQHLRGAVKGIADALLIVSEPFAVAYGMDALLHTMVIDIGA
                     GTTDFCVMNGRYPTEEDQRTLTHAGDSIDDQLMKLVKTKAPDGRFSIHMIREWKEKFS
                     FVGDSKQPVVVRFPVKGKPADVDITAEMKKACESIVGPIAETMLDLLSKVEPEYQEKV
                     QRNVILAGGGSQIPGLAAALEKVLLDVGGGRVRAVEDPVFAGANGGLAIAIDAPEGEW
                     EPLGA"
     gene            complement(2224..3219)
                     /locus_tag="PLB01_15720"
     CDS             complement(2224..3219)
                     /locus_tag="PLB01_15720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQQ78802.1"
                     /translation="MASTGRLKQTLVRDAIRENVDVIEGRLPKGLRRSDRVRRAIFRA
                     SVFVIVPLALFLSINALTTGKGERTDVVYVSTPAAPPAPAAAVLHAPKGLEPSLFRLS
                     VRSVVLDPGHGGTDPGATGAGGVREKDVTLDVATRVKALLEQGHLAVSLTRDGDTTLS
                     LKDRVLLANAKKPDLFVSIHVNSLPAVRDKRVVETYVLGTTNDARVQALAGDENRESG
                     YTLADYRRLLEGVLQDARSGESKRLAESVQGGLVAGLRPSVPRLEDHGVKEAPFVVLI
                     ATEMPGILAEVSTVSNDDEARRLKDSAYRQTIARALADGILAYAESRNHPPSKRS"
     gene            3306..3866
                     /locus_tag="PLB01_15725"
     CDS             3306..3866
                     /locus_tag="PLB01_15725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQQ78803.1"
                     /translation="MKRLIPIGVCSLVLILFSAAPAAAQGTGGFQFGLSGGASFPTSD
                     WSDAYGTGYNGGIVLNYELPALPLGIRVDGDYRNFSAKTYGAFSNSAEIFDGNANLVV
                     GIRILLVKVYALGGGGVYNMKFKSESAGVSSSISQTDFGWNAGAGVAFVVGKVSIFGE
                     GRYHEVTLDNSAGKFKFVAATAGILF"
     gene            complement(3870..4700)
                     /locus_tag="PLB01_15730"
     CDS             complement(3870..4700)
                     /locus_tag="PLB01_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ78804.1"
                     /translation="MSASAARFLAIEGLCLSRGGTDVLKGVTLAVSRGELVALMGLSG
                     GGKTTVLRAVVGLEGFGAGTIDVERVALRPGPPPAGETLKALHARVGMVFQYHGLFDH
                     LSVLDNVTLAPLHVAKLERTAAEARALALLASLGVERHARAYPRELSGGEAQRVAIAR
                     TLAMEPPLLLMDEPTASLDPARRNELGRSLTRLVREEGRTLVVATHDDDLVRDFATRV
                     VILADGAVVEEGPPGKVLTDPQHPATRFLLQTKDRGPAPERPPKARGPRVPRGRAPAA
                     "
     gene            complement(4697..>6164)
                     /locus_tag="PLB01_15735"
     CDS             complement(4697..>6164)
                     /locus_tag="PLB01_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HQQ78805.1"
                     /translation="EGGAPFVEADPTDPSKLRGFDVEIAALVARKLGRTPAFVQVAFP
                     SLDQSAARGDFDLGLSGIEDTPARRAALAVTIPYYEFREVLTVRAADVGRFHGLADLK
                     GRRVGTLAGTIAWDALVEAEKRDGIAPVSYDDDVHPYEDLAKGRLDAVLLDHVLAARS
                     VRRVAGLAIQPATVATGRYVAILAKENGPLRDRIDGILLDAMRDGTLKEIFERWGVWD
                     EEQASYQEKTKKEISLNGNRVGGAAGAPRASEQQAAAETLPSSPSKRSSSFFSSSSLS
                     LVVSYLPSLLKASLVTITLSCLAMALAVVLGVCIASGRVYGSAPVRFVLTAYVEVTRG
                     TPVLLQLFVLYYGLSSVVRLPAFVAALLGLGLNYAAYESEIYRGALEAVPKGQLEAAR
                     TLGLTEGQVLRLVRGPQAFRLALAPMTNDFVALLKDSSLVSVVTVVELTKQTAIFATN
                     IASWAVPGALCAALYLAMSLPLARLARRLEGRWKAASA"
BASE COUNT          920 a         2118 c         2237 g          889 t
ORIGIN      
        1 agcctcgacc ttcttcggcg ccggagcggg cgcctccacc ttcttcacga cgtctccgga
       61 caggaaggtc cggacggccc aggtgaggcc gccggcgacc acgaggacga cggcgatcgc
      121 gacgatccag cccgtcatcc cgctcttctt cgcaggcgcg ccctcggccg ccttgaacgc
      181 gaactcctcc atcgccggct cgaccggctc cgtcacgtcc ccgacgaggt tcgtgagcgg
      241 cggcatctcc gacagggact tgacgggcgg gtcgattcgc cgcaccggcg gctcgaccca
      301 cggtgccgga atctcgaccg ggggagcggg aggctcgaaa cgcggcgcgg cggccccgag
      361 aggctcgctc gcagcctcga acgccggctc gtcctgcggt aaggcccagg gatccggccg
      421 cgcgaagtcg ggctcctcga cgcgcggcgg agacggcgga gacggcggct ccggaggcgt
      481 gaaggccggg aactcggaga gcgccggcgc ctcgggcggg gcaggctcgg tcgcacgggg
      541 cggaggcgcc ggggcgagag gcgacgaaag ctcgagcgtc ggcgacgaga acaacgggag
      601 gtcgtccacc ggggccttcg ggcgcgacgc cgcgccgctg cgctccatgg cgaacccttc
      661 cggcgcgacg acccgatccg gcgtgggcag ccgcggagga gacggcgggg cagaagcggg
      721 aacgacggtg ctttccggct gcgcgattcc ccggacgcgc gagcgcgggg gcagcggaag
      781 gatcgccgac cggaacggcg cgtcgtccgg ctggatgttg aggtccttct cccactcgag
      841 gtcggccgag ccggctccct ggaacgggtc gtcggccggc ggggaggcag gcttccttgg
      901 cttgagcggc ggcgccggga tcgagaccga cctgggctca tccggctcct cggcggccgg
      961 ggccgcgacc ccttcctgct cgagctggcg gcgggcccgc cgcacgagca cgtcgagcgg
     1021 cgcggactcg aagccctgct tgaccttccc gagctcgtcc gcgacgaccg agcgcgccgc
     1081 cttgaacagc acggccgggt ccgcggagac ggtgcgcgcc agttcacgca cgacgtccgt
     1141 cacggcgggc agcatgcgcc gcttcggcgt cccggagtcg cggccgaatc cgccgtcgcc
     1201 tctcatgcgc cgaggggctc ccactcccct tcgggggcgt cgatcgcgat ggcgagaccg
     1261 ccattcgcgc ccgcgaagac cgggtcctcc acggccctca cgcgcccgcc gccgacgtcg
     1321 aggagcacct tctcgagcgc ggccgcgagg cccgggatct gcgagccgcc gcccgcgagg
     1381 atgacgttgc gctggacctt ctcctggtac tcgggctcca ccttggagag gaggtcgagc
     1441 atcgtctcgg cgatgggccc gacgatgctc tcgcaggcct tcttcatctc ggccgtgatg
     1501 tcgacgtcgg cgggcttgcc cttgacggga aagcgcacga ccaccggctg cttcgaatcg
     1561 ccgacgaagc tgaacttctc cttccactcg cggatcatgt ggatggagaa gcggccgtcc
     1621 ggcgccttcg tcttcacgag cttcatgagc tggtcgtcga tggaatcacc cgcgtgcgtc
     1681 agcgtccgct ggtcctcctc ggtcgggtag cggccgttca tgacgcagaa gtccgtggtg
     1741 cccgcgccga tgtcgatgac catcgtgtgc aggagcgcgt ccattccgta ggcgaccgcg
     1801 aacggctcgg agacgatcag gagcgcgtcc gcgatgccct tcacggcgcc gcgcaggtgc
     1861 tggcgggaga cgcgcagcgc ctctgcgggc acgccgacca ccgcctcgac gcgctggccc
     1921 tccacgcggc cggccagcgc gatcaggtgc cggagaagtt cgtgcacggc ctcctcgtcc
     1981 ttcgcggagc cctgcttgat gaggccgcgc tcgagcgggc gatgaaggtc gagcatcgac
     2041 cgcgcgtcga gcgcctcggc gccgaacagg atcgtcttct tcacgatctt ccgcgcgacc
     2101 atgtcgagcg gccagcccac gtagctttcc acgacgtgct tcgtgccgtt cgacgccgcg
     2161 atcgcgctgc gcgaggtgcc gaggtcgatg ccgacgtgga ggacgtcttt cttcgaggtc
     2221 atgtcaactc cgtttcgagg ggggatgatt gcgggattcc gcgtacgcga ggatcccgtc
     2281 cgcgagggcg cgtgcgatcg tctggcggta ggcggagtcc ttgaggcgcc gcgcttcgtc
     2341 gtcgttggag accgtggaaa cctccgccag gatgcccggc atctccgtcg cgatgaggac
     2401 cacgaacggg gcttccttca cgccgtggtc ctcgaggcgc gggacggagg gcctgaggcc
     2461 cgcgacgagg ccgccctgca cggactcggc gagccgcttc gactcgccgg agcgcgcgtc
     2521 ctgaagaacg ccctcgagga ggcgccggta gtccgcgagc gtgtagcccg actcgcggtt
     2581 ctcgtcgccc gcgagggcct ggacgcgcgc gtcgttcgtc gttccgagga cgtaggtctc
     2641 cacgacgcgc ttgtcgcgca cggccgggag ggagttcacg tggatcgaga cgaaaaggtc
     2701 gggcttcttc gcgttcgcga ggaggacgcg gtccttgagg gacagcgtcg tgtcgccgtc
     2761 gcgcgtgagc gagacggcga ggtggccctg ctcgaggagc gccttgacgc gggtcgcgac
     2821 gtcgagcgtg acgtccttct cgcgcacgcc gccggcgccc gtcgcgccgg ggtcggtccc
     2881 gccgtgcccc gggtccagga ccacggaccg gacggaaagc cggaacagcg acggctcgag
     2941 gcccttcggg gcgtgaagga cggccgctgc gggcgccgga ggggctgccg gcgtcgagac
     3001 gtagacgacg tccgtgcgct cgcccttgcc cgtcgtgagc gcgttgatcg agaggaagag
     3061 cgcgagcggc acgatgacga agaccgaagc gcggaagatc gcgcggcgga cccggtcgga
     3121 gcgcctgagg cctttcggaa gacgcccctc gatcacgtcc acgttctcgc ggatcgcgtc
     3181 cctgacgagg gtctgcttga gccgtccggt cgacgccatg atttccgtga aaaggttaac
     3241 actcggggac ttaagggcca gcggcgggtt taggatgcgt gtgtgaattc ttcgaaggag
     3301 agaacgtgaa gagactcatt ccgatcggcg tctgcagcct ggtcctcatc ctgttttccg
     3361 ccgctccggc cgccgcccag gggaccggag ggttccagtt cggcctctcg ggaggcgcct
     3421 cgttcccgac gagcgactgg tccgacgcct acggcaccgg ctacaacggc ggcatcgtgc
     3481 tgaactacga gctcccggcg ctgccgctcg gaatccgcgt cgacggcgat tacaggaact
     3541 tctccgccaa gacctacggc gccttttcga attccgcgga gatcttcgac gggaatgcga
     3601 acctcgtcgt cgggatccgc atcctcctcg tcaaggtcta cgccctcggc ggaggcggcg
     3661 tctacaacat gaaattcaag tccgagagcg cgggcgtctc gtcgtcgatc tcccagaccg
     3721 acttcgggtg gaacgcgggc gccggcgtcg cgttcgtcgt gggcaaggtc tcgatcttcg
     3781 gcgagggccg gtatcacgag gtcaccctcg acaactcggc cggcaagttc aagttcgtcg
     3841 ctgcgaccgc gggaatcctc ttctagcctt caggccgcgg gcgcccgtcc ccgcgggacg
     3901 cgcggcccgc gtgccttggg cgggcgctcg ggcgccgggc cgcggtcctt cgtctggagc
     3961 aggaagcgcg tcgcggggtg ctgcgggtcc gtgaggacct tgccgggcgg cccctcctcc
     4021 acgacggccc cgtccgcgag gatcacgacg cgcgtcgcga agtcgcgcac gaggtcgtcg
     4081 tcgtgcgtcg cgacgacgag cgtccggccc tcctcccgca cgagcctcgt gagcgaccgc
     4141 ccgagctcgt tccgccgcgc cgggtcgagg gaggcggtcg gctcgtccat gaggaggaga
     4201 ggcggctcca tcgcgagcgt gcgggcgatt gcgacgcgct gcgcctcgcc acccgaaagc
     4261 tcccgcggat aagccctcgc gtgccgctcg acgccgaggg acgcgaggag ggcgagcgcc
     4321 cgtgcctcgg cggccgttcg ctccagcttc gcgacgtgga gcggcgcgag cgtcacgttg
     4381 tcgaggaccg agaggtggtc gaagagaccg tggtactgga agaccatccc gacccgggca
     4441 tgaagcgcct tcagcgtctc cccggccggc gggggaccgg gtcggagcgc gacgcgctcg
     4501 acgtcgatcg tcccggcgcc gaagccctcg aggccgacga ccgcccggag cacggtcgtc
     4561 tttccgccgc cggagagccc catgagggcg acgagctcgc cgcgcgagac ggcgagcgtg
     4621 acgcccttga ggacgtccgt gccgccgcgc gagaggcaga ggccctcgat cgcgaggaac
     4681 cgggcggcgg acgcgctcac gccgacgccg ccttccagcg cccctcgagg cggcgcgcga
     4741 ggcgtgcgag cgggagcgac atcgcgagat acagggccgc gcagagcgcc ccgggcacgg
     4801 cccagctcgc gatgttcgtc gcgaagatcg ccgtctgctt cgtgagctcg acgaccgtga
     4861 cgacggacac gagcgaggag tccttcagga gcgcgacgaa gtcgttcgtc atgggcgcga
     4921 gcgcgaggcg gaacgcctgc gggccgcgca cgaggcgcag cacctggccc tccgtgaggc
     4981 cgagcgtccg cgcggcctcg agctggccct tcggcaccgc ctccagcgcg ccgcggtaga
     5041 tctcgctctc gtacgcggcg tagttgaggc ccagcccgag gagcgccgcg acgaacgcgg
     5101 gcagtcgcac gacggaggag aggccgtagt agaggacgaa gagctgaagg agaacgggcg
     5161 tcccgcgggt gacctcgacg tacgccgtca gcacgaagcg caccggcgcg ctcccgtaga
     5221 cgcgcccgct cgcgatgcag acgccgagca cgacggcgag cgccatcgcg aggcacgaaa
     5281 gcgtgatcgt gacgagggac gccttgagaa gagacgggag ataggagacg acgagagaaa
     5341 gagaggagga agagaagaaa gaagaagatc tcttagaagg tgaagagggg agggtctcgg
     5401 ctgcggcctg ctgctcgctc gcacgcggcg ctccggcagc ccccccaacc ctattaccat
     5461 tcaaagaaat ctctttcttt gtcttttcct gatacgaagc ctgctcctcg tcccagactc
     5521 cccatctctc gaagatctcc ttcagcgtcc cgtccctcat cgcgtcaagg aggatcccgt
     5581 cgatgcggtc gcggagcggc ccgttctcct tcgcgaggat cgcgacgtac cggcccgtcg
     5641 cgacagtggc gggctggatc gcgagacccg cgacgcggcg cacggagcgc gccgcgagga
     5701 cgtggtcgag gaggacggcg tcgaggcggc ccttggcgag gtcctcgtac gggtggacgt
     5761 cgtcgtcgta ggacacgggc gcgattccgt cccgcttctc ggcctcgacg agcgcgtccc
     5821 acgcgatcgt gccggcgagc gtcccgacgc gccgcccctt gaggtccgcg aggccgtgga
     5881 accggccgac gtcggccgcg cggacggtca ggacctcccg gaactcgtag tacggaatcg
     5941 tcaccgcgag cgccgcgcgg cgcgccgggg tgtcttcgat gcccgagagg ccgaggtcga
     6001 agtcgccacg ggcagccgac tggtccagag acgggaacgc gacctggacg aacgcgggcg
     6061 tccgtccgag cttcctcgcg acgagcgccg cgatctcgac gtcgaagccg cgcagcttcg
     6121 acgggtccgt ggggtcggcc tcgacgaagg gcgcgccccc ttcc
//
LOCUS       DAOMFY010000244         6053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4391_length_6053_cov_7.514171, whole genome shotgun sequence.
ACCESSION   DAOMFY010000244 DAOMFY010000000
VERSION     DAOMFY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4391_length_6053_cov_7.514171"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLB01_15740"
     CDS             complement(<1..957)
                     /locus_tag="PLB01_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ78806.1"
                     /translation="MKIGKAFGFGFGRGFGFGISLKVVSGLLAAGVVSAASAGCSGPH
                     SPSKKDLAPVAAATAIVERASLPDRVALYGTVTADRSAAVSSRVMANVVAVPARVGQS
                     VAAGDVLVEIDAATASGQVAQAGGALAQARAALSLAERNYERYKALAASGSASALELD
                     MARMQYEQAKGAVQQGEGAVEAASSVAKESRVTAPFAGRVAARLVEAGDLAAPGRPLV
                     TIESATGRRLVVQVPAGTVATSGLSTGRKLSVKIDGVAAELTGTVVEMSPGADPASHT
                     FTAKIELAGAPAATGLTGRAFVRAAVRKTVLVPAAAVGDGVDI"
     gene            complement(954..2336)
                     /locus_tag="PLB01_15745"
     CDS             complement(954..2336)
                     /locus_tag="PLB01_15745"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQQ78807.1"
                     /translation="MNDKPPVAARAVFLAAALLAPSLARSETAAGAAPPLTLAGAMQK
                     AREGAREVVAAQARAGAAEARASQAKGFRWPSLNLTALWMRTNNPAEVFALKLNQGVF
                     SFPEFVASNPNDAAPLNTGITRAELVLPLFTGGELSGRIDQAKLAADAASGTSTWVAD
                     NAALAAAEAYVMVAQAEEYAALLQKSRDTVAAHVKLAGDYAAQGMLVRSELLRAEVEL
                     SKMDDLVTEADGRVRVANANLAFRLGAAGSSSWALAPLPDPRPVATPVDGWLATAAER
                     SDLGSARKLLAAGELEEKVKKAPIWPKLALVAKGELYGDKPFGSTGTSGSIMAVASWN
                     VFAGGSDHAAAVAAREDARAGREDVARFAEGVRLEVRQAYEEAKTAEARHETAKKALE
                     SAREAERITNERYRAGVVKTLDLLDVTTARREAETRELVARADAHTAALKLAVKAGRR
                     PESVLNGGTE"
     gene            2409..2642
                     /locus_tag="PLB01_15750"
     CDS             2409..2642
                     /locus_tag="PLB01_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQQ78808.1"
                     /translation="MTINDALRGLGGFFTLASVALGYWVHPGFFLFTAFVGANLLQSA
                     FTKTCPMVPILKKLGLKETLDQPQASCCAEKAA"
     gene            2639..3025
                     /locus_tag="PLB01_15755"
     CDS             2639..3025
                     /locus_tag="PLB01_15755"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQQ78809.1"
                     /translation="MRYDVAFPGGVAVEASAKGHTIRTDQPEPFGSDSAPAPFDLFIA
                     SIATCMGYYALRFCQERGIPTEGLGLSVETVKDPEKKRLSTIRAVLTLPPGFPEKYAG
                     AIQRAVDQCAVKKHLVEPPAFELTVN"
     gene            complement(3034..3741)
                     /locus_tag="PLB01_15760"
     CDS             complement(3034..3741)
                     /locus_tag="PLB01_15760"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQQ78810.1"
                     /translation="MSSLKLSFFDRWLRRGIITSAAFGFTLPYFRLLNRVQATGDAIL
                     DTLPRKNVVFLSNHQTYFMEAFAFFDLVYVKHALPLEDPFTRFSAATETMQKNLVTQL
                     FTKAGGVTFRRSFREGGKDVKRSVDFEGIAKVEEAIATGWLLHFPAGTTQKGAPLRPG
                     VAQLLHRTKAVAVPVRVDGFRELLLHKQLPGKFLRKCHLTLHPPMDLDAFYAAPYDKE
                     SGAEVVERLSAIIADPA"
     gene            complement(3771..4280)
                     /locus_tag="PLB01_15765"
     CDS             complement(3771..4280)
                     /locus_tag="PLB01_15765"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ78811.1"
                     /translation="MSFPPDDLAALYAFNAWANGRVVEALRTIPEHDYEKEQGGGWPS
                     LRATFVHIAGATDAWAERFAGNDATRLPTIHDLPTLDDAVRVLLAAQEKHAKLLVTYD
                     TARLAGPFTWKNLKGEEKTAPFWVVVRHVVNHQTYHRGQISSMLRRLGHAPKATDMVV
                     WGIEIASGA"
     gene            complement(4277..5284)
                     /locus_tag="PLB01_15770"
     CDS             complement(4277..5284)
                     /locus_tag="PLB01_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQQ78812.1"
                     /translation="MYNFIGMKLVSFLPPEGEASQSGVLRRHPRLGAVIGDDRILDFA
                     RAHPRFANVSMLDLVKRSGELLPEAASLVAMASELPREAFWSEATVTLAAPLPRPVSI
                     RDGYAFRQHVETARRNRGLPMIPEFDDFPVFYFTNALAVVGPGELRVGGRRLERLDFE
                     LECFCVIGQRVTNPTLEEADAAIFGYGVMNDFSARALQMEEMKLNLGPAKGKDFATGL
                     GPWLVTKDELAPRLRPSHKGNVLYAAMSATVNHVPVSHGDVSQMNWTFAEIVQRAADG
                     VTLEPGEVIGSGTVGTGCFLELNGSKVTHDQWLKPGDVVSLTIDGLGTLTNTIVEDPA
                     R"
     gene            5318..>6053
                     /locus_tag="PLB01_15775"
     CDS             5318..>6053
                     /locus_tag="PLB01_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78813.1"
                     /translation="MPRAVGRDRLGVETAGQVLLDCDAPKGWRARTPATPTSAEHPGT
                     ALSWEGEFFEVLEVRSRGSGVQYVLAPWDDRHAMRVVMAYNEDTEAARGRERTDAARR
                     SDRYVYLLLGAPLIGSLPGHVQEFFEREYNVPARRLSLASALPLWIVGWLALVLLLAS
                     AVGGAGAPPLPVLVFGVYLLAESTARLAVCLLQGRAIGTFVGTLAWEVWRRLKTAFGG
                     TPLPAERSIWDVESDATQDVLDRYHVL"
BASE COUNT          890 a         2051 c         2179 g          933 t
ORIGIN      
        1 gatgtcgacg ccgtcgccta cggcggcggc ggggacgagg accgtcttcc ggaccgcggc
       61 gcggacgaag gcgcggccgg tcaggcccgt ggcggcgggc gcccccgcca gctcgatctt
      121 cgccgtgaac gtgtggctcg ccgggtccgc gcccggggac atctcgacga cggtgcccgt
      181 gagctcggcg gcgacgccgt cgatcttcac ggagagcttc cgtccggtcg agaggcccga
      241 ggtggcgacc gtgcccgcgg gaacctgtac gacgaggcgt cggcccgtcg cggattcgat
      301 cgtgacgagc gggcggcccg gggccgcgag gtccccggcc tcgacgaggc gcgccgccac
      361 gcggcctgcg aagggagccg tcacgcggga ctccttcgcg acggaggagg cggcttccac
      421 ggcgccctca ccctgctgga cggcgccctt cgcctgctcg tactgcatgc gggccatgtc
      481 gagctcgagg gcggaggccg agccggatgc cgcgagggcc ttgtagcgct cgtagttgcg
      541 ctcggccagg gagagcgccg cacgcgcctg ggcgagagcg cccccggcct gagcgacctg
      601 tccgctcgcg gtcgcggcat cgatctcgac gaggacgtcg ccggcggcga cggactgccc
      661 gacgcgcgcg gggacggcga cgacgttcgc catcacgcgc gacgaaacgg cggccgagcg
      721 gtcggcagta acggtgccgt agagagcgac gcgatcggga agacttgccc gctcgacgat
      781 ggcagtcgca gccgcaaccg gagcgagatc cttcttagaa ggtgaatgcg ggccggagca
      841 gcctgcggag gctgccgaga caactcccgc cgcgagcagc ccgctaacta ctttcaaaga
      901 aattccgaat ccgaatcccc gtccgaatcc gaaaccaaac gcttttccga tcttcactcc
      961 gttcctccgt tcagaacgct ctcggggcgg cgccccgcct tgacggcgag cttcagcgcc
     1021 gccgtgtgcg cgtcggcgcg ggcgacgagc tcgcgcgtct ccgcctcgcg gcgggccgtg
     1081 gtgacgtcca ggagatcgag cgtcttcacg acgcccgccc ggtacctctc gttcgtgatg
     1141 cgctcggcct cgcgggccga ttcgagcgcc ttcttcgcgg tctcgtgccg cgcctcggcc
     1201 gtcttcgcct cttcataggc ctggcggacc tcgaggcgca cgccttcggc gaaccgggcg
     1261 acgtcctcgc ggcccgcgcg cgcgtcctcg cgcgccgcga cggcggccgc atggtcgctg
     1321 ccgcccgcga acacgttcca ggacgcgacc gccatgatgg agccggacgt gcccgtcgat
     1381 ccgaacggct tgtcgccgta gagctcgccc tttgcgacga gcgcgagctt cggccagatg
     1441 ggagcctttt tcaccttctc ctcgagctcg cccgcggcga gaagcttccg cgcagagccg
     1501 aggtcggagc gctcggcggc ggttgcgagc cagccgtcca cgggtgtcgc gaccgggcgc
     1561 gggtcgggaa ggggagcgag ggcccacgag gagctgccgg ccgcgccgag ccggaacgcg
     1621 aggttcgcgt tcgcgacgcg cacgcggccg tccgcctccg tgacgaggtc gtccatcttg
     1681 gagagctcga cctcggccct gaggagctcc gagcggacga gcatgccctg ggcggcgtag
     1741 tcgccggcga gcttgacgtg cgcggcgacc gtgtcgcgcg acttctggag gagagcggcg
     1801 tattcctcgg cctgggcgac catgacgtac gcctcggcgg ccgcgagggc cgcgttgtcg
     1861 gccacccacg tggacgtgcc cgaggccgcg tcggccgcga gcttcgcctg gtcgatccgg
     1921 cccgagagct cgccgcccgt gaagaggggg aggacgagct cggcccgtgt gatgcccgtg
     1981 ttcaggggcg ccgcatcgtt cgggttcgaa gcgacgaact ccgggaacga gaagacgccc
     2041 tggttgagct tgagggcgaa gacctcggcc gggttgttcg tgcgcatcca gagcgcggtc
     2101 aggttcaggg acggccaccg gaagcccttc gcctgcgagg cgcgggcctc ggcggcgccc
     2161 gcgcgggcct gggcggcgac gacctcccgt gcgccttccc gggccttctg catggcgccg
     2221 gcgagcgtga ggggcggagc ggccccggcc gccgtctcgg accgggcgag ggagggcgcc
     2281 aggagggcgg ccgccaggaa gacggcccgg gcggccacgg ggggcttgtc attcatagaa
     2341 ttctcttata taactatata gtgattgaag tcaaccggca ggatgccggc ataaacaagg
     2401 agaacacgat gaccatcaac gacgccctcc gcggactcgg cggtttcttc accctggcct
     2461 ccgttgcgct cggatactgg gttcaccccg gcttcttcct cttcacggcc ttcgtggggg
     2521 cgaacctgct ccagagcgcc ttcacgaaga cctgcccgat ggtcccgatc ctgaagaagt
     2581 tagggctgaa ggagacgctt gaccagcccc aggcgtcctg ctgcgccgag aaggccgcat
     2641 gagatacgac gtggccttcc cgggcggcgt cgccgtggag gcgtccgcca aggggcacac
     2701 gatccggacg gaccagcccg aacctttcgg ctcggactcg gccccggcgc ccttcgacct
     2761 cttcatcgcg tcgatcgcga cgtgcatggg ctactacgcc ctgcgcttct gccaggagcg
     2821 gggaatcccg acggaaggcc tcgggctctc ggtcgagacc gtcaaggatc ccgagaagaa
     2881 gcgtctctcg acgatcaggg cggtcctcac gctcccgccg ggcttcccgg agaagtacgc
     2941 aggcgcgatc cagagggccg tcgaccagtg cgccgtcaag aaacacctgg tcgagccgcc
     3001 cgcgttcgag ctgacggtga actgaagaac cggtcaggcc gggtcggcga tgatcgcgga
     3061 gaggcgttcg acgacctccg cgccggattc cttgtcgtac ggcgccgcgt agaacgcgtc
     3121 gaggtccatg ggaggatgga gcgtcaggtg gcatttccgg aggaacttgc cggggagctg
     3181 cttgtgcagg agcagctcgc ggaatccgtc cacgcgcacg ggcacggcga ccgccttcgt
     3241 gcggtggagg agctgggcga cgccgggtcg cagcggcgcg cccttctggg tcgtccccgc
     3301 ggggaaatgc aggagccagc cggtcgcgat cgcttcctcg accttcgcga tgccctcgaa
     3361 gtccacggag cgcttcacgt ccttcccgcc ctcgcggaag ctgcgccgga acgtcacgcc
     3421 gccggccttc gtgaagagct gggtcacgag gttcttctgc atcgtctcgg tcgcggcgga
     3481 gaagcgcgtg aacgggtcct cgagaggcag ggcgtgcttc acgtacacga ggtcgaagaa
     3541 cgcgaacgcc tccatgaagt acgtctggtg attggacagg aagacgacgt ttttccgggg
     3601 gagcgtgtcg aggatcgcgt cgccggtcgc ttggacgcgg ttcaggaggc ggaagtaggg
     3661 cagcgtgaac ccgaacgccg cgctcgtgat gatgccgcgg cggagccagc ggtcgaaaaa
     3721 cgacagtttc agcgaggaca tgtttacgcg attgtaaggg gaggccggac tcaggcgccg
     3781 gaggcgatct cgatccccca gacgaccatg tccgtcgcct tcggggcgtg cccgagccgg
     3841 cgcagcatcg aggagatctg gccgcggtgg taggtctggt ggttcacgac gtgccggacg
     3901 acgacccaga agggcgccgt cttctcctcg cccttgaggt tcttccacgt gaagggtccc
     3961 gcaaggcgcg ccgtgtcgta cgtcacgagc agcttcgcgt gcttctcctg cgccgcgagg
     4021 aggacgcgga cggcgtcgtc gagcgtgggc aggtcgtgga tcgtcggcag acgcgtcgcg
     4081 tcgttcccgg cgaagcgctc ggcccacgcg tccgtcgcgc ccgcgatgtg aacgaacgtg
     4141 gccctgagcg agggccagcc gccgccctgt tccttctcgt agtcgtgctc cggaatggtg
     4201 cggagggcct cgacgacgcg cccgttcgcc caggcgttga acgcgtagag ggcggcgagg
     4261 tcgtcggggg ggaagctcat cgcgcggggt cctccacgat cgtgttcgtg agcgtcccga
     4321 ggccgtcgat cgtgaggctc acgacgtcgc cgggcttcag ccactggtcg tgcgtgacct
     4381 tcgagccgtt cagctcgagg aagcatcccg tcccgaccgt gcccgatccg atcacctcgc
     4441 cgggctcgag cgtgacgccg tcggcggcgc gctgcacgat ctcggcgaac gtccagttca
     4501 tctgcgagac gtcgccgtgc gaaacgggca cgtggttgac cgtggccgac atggccgcgt
     4561 agaggacgtt gcccttgtgc gacgggcgga gcctcggcgc gagctcgtcc ttcgtgacga
     4621 gccacgggcc gaggcccgtc gcgaagtcct tccccttggc gggtccgagg ttcagcttca
     4681 tctcctccat ctggagggcg cgcgccgaga agtcgttcat gacgccgtac ccgaagatcg
     4741 cggcgtcggc ctcttcgagc gtcgggttcg tgacgcgctg gccgatcacg cagaagcact
     4801 ccagctcgaa gtccagcctc tcgaggcggc ggcccccgac gcggagctcg ccggggccga
     4861 cgaccgcgag ggcgttcgtg aagtagaaga cggggaagtc gtcgaactcc gggatcatcg
     4921 ggagcccgcg gttcctccgg gccgtctcga cgtgctggcg gaacgcgtag ccgtcgcgga
     4981 tcgagacggg cctcggcagc ggggcggcga gcgtgacggt cgcctcggac cagaacgcct
     5041 cgcgcggcag ctcggaagcc atggcgacga gcgacgccgc ctcggggagc agctcgccgg
     5101 accgtttcac gaggtcgagc atcgacacgt tcgcgaaacg cggatgggcg cgtgcgaagt
     5161 cgaggatcct gtcgtcgccg atcacggcgc cgagccgcgg gtgccggcgg aggacgcccg
     5221 actgggaggc ttcgccctcg ggaggcagga acgagacgag cttcatcccg atgaagttat
     5281 acaccccgcg ctaacatcag gggacggagg aacgggcatg ccgcgcgccg tgggacgcga
     5341 ccgcctcggg gtcgagaccg ccgggcaggt ccttctcgac tgcgacgctc cgaagggctg
     5401 gcgcgcccgc acgccggcga cgcccacctc cgccgagcac cctgggacgg ccctttcgtg
     5461 ggaaggggag ttcttcgaag tcctcgaggt gcggtcccgc ggatcgggcg tccagtacgt
     5521 cctcgcgccg tgggacgacc gccacgcgat gcgcgtcgtc atggcctaca acgaggatac
     5581 ggaggcggcg cggggccgcg agcggacgga cgcagcgcgc cgttcggacc gatacgttta
     5641 cctcctcctg ggcgcgcctc tcatcggcag cctcccgggg cacgtccagg agttcttcga
     5701 aagggaatac aacgtccccg cgcgacgcct gagcctcgct tctgccctcc cgctctggat
     5761 cgtcgggtgg ctcgctctcg ttcttctcct cgcctccgcc gtcggtggcg ccggcgcgcc
     5821 gccgctgccg gttctcgtct tcggcgtgta tctcctcgcc gaatccacgg cccgtctcgc
     5881 cgtgtgcctg ctgcagggac gcgccatcgg gactttcgtc ggcacgctcg cgtgggaggt
     5941 ctggaggcgc ctgaaaaccg ctttcggagg aactccgttg cccgccgagc ggtcgatatg
     6001 ggacgtcgag tccgacgcga cgcaggacgt cctcgaccgt taccacgtcc tcg
//
LOCUS       DAOMFY010000245         6005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4441_length_6005_cov_7.519664, whole genome shotgun sequence.
ACCESSION   DAOMFY010000245 DAOMFY010000000
VERSION     DAOMFY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4441_length_6005_cov_7.519664"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLB01_15780"
     CDS             <1..856
                     /locus_tag="PLB01_15780"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQQ78814.1"
                     /translation="EQVATWRQMDETIRARYAAGMGTQQDVIRAQSEQTRLLQQRRRD
                     QAAEEGALAELRRLLDFSPEAPVAAAARLSPGVSLAVPGRDEAVAKALGATPEIRDAA
                     ALGERSRFAADLAKRGTQPDFVVSAAYMNRGSLPLMWAASVGVSVPIFAGARTRPLVA
                     EAQGLSDSAARAEAALKSIARARAEERLIRMRQLADEARLDADGVLVQDRLSVDAALA
                     SYRSGAVPFVTVLEALGTFYQDRRAAVGRIAGFLRADADLREYALDRTAATAGSSTPS
                     SSTSGAGM"
     gene            867..2144
                     /locus_tag="PLB01_15785"
     CDS             867..2144
                     /locus_tag="PLB01_15785"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ78815.1"
                     /translation="MNHLRRNLHSLPALAAVAALLLFAPACGSKKPSSGGGAAAKAAK
                     WQCPMHPEVVKDGPGDCPICGMKLVPIEEAAPAAPKSAGAGPEGLATVTIDAAKRQLL
                     GLKTVAVTRAPFATSIRTTGRIAADERRVHHVHTRYEGFVEHVTADFTGKYVHRGEVL
                     ALIYSPELYATQQEYLLALKASRSLGPSGVPSVAQGGRDLLEAARQRLLLWEITPADI
                     AAIEKKGEPIRAMPVYAPISGYVTGRTAYHGMKVTSADTLFDILDLSAVWVLADVYEY
                     ELPRLAAGEKATMTLSYWPGRSWQGKVTYVYPSVDEKTRTVKVRAEFDNPKGELKPEM
                     FADVTLHAKPRSVLQVPDDAVLESGTRNIVFVTEGEGALAPREIALGDRGEGMVEVRS
                     GLKEGDVVVRGANFLVDSESRLKAAISAMGTSR"
     gene            2141..5401
                     /locus_tag="PLB01_15790"
     CDS             2141..5401
                     /locus_tag="PLB01_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQQ78816.1"
                     /translation="MIRRIIRFSAENKYLVIALYAIVIVLAIGVIRRTPLDAIPDLSD
                     TQVIVYSRWDRSPDVLEDQVTYPIVASLLGSAKVKTIRGFSDFGFSYVYVIFEDGTDI
                     YWARSRVLEKLSTIQSRLPAGVKTELGPDATSVGWIYQYALVDESGRNATDDIRSYQD
                     WFLRYAVQSVPGVAEVASVGGFQKQYQVTLDPNTLSAYGIPLESVVEAIRKSNNEIGG
                     RLIEFSGREYMVRGRGYLKTVEDIGRIVVKTNEKGTPLTVADVGRVALGPEIRRGVTD
                     LDGKGDAVGGIVVMRSGENALDVIDRVKARLKELEPSLPPGLKIVTTYDRSDLIKRSV
                     DTLRSKLIEEIIVVSLVILVFLWHVPSAIVPIVTIPISIVLAFIPFNAMGLNINIMSL
                     GGIAISIGILVDGAIVEVENAYKKLQEWNDGGRPGDFHKVRLEALMEVGPSVFFSLLV
                     VAVAFLPVFTLVDQEGRLFKPLAFSKNLAMAIAAFLAITLDPAIRMLFTRMDGFSFRP
                     RWLAWIVNHAVVGKYYAEERHPISVVLHRLYERPCRAVLRHPRKTIAIALLALLATVP
                     VFLKLGSEFMPPLYEGSILYMPTTLPGISVTEAERLLQTQDRVLKSFPEVERVFGKAG
                     RAETSTDPAPFSMMETTIILKPESEWRGKDRWYSSWAPGWLAGVFRHVWRDRITHDEL
                     VDEMDRALKIPGTTNAWTMPIKARIDMLSTGIRTPVGIKIFGSSLGEIERIGAEVEAT
                     VKSVSGTRSAFAERVAGGYFVDFDLNRDALARYGLTVDDANAVIMTAIGGETVTTVIK
                     GRERYSVNVRYSRELREDFDRLARVLVPTMGGAQVPLGQLATIRQVRGPAMIRNENGL
                     LSGYVYVDFDTSKEDVGGYVERAKKAVAAHVTLPPGYALVWSGQYENMLRVKERLKVV
                     IPVTLLLIWLLMYANTKSAMKTMIVLLAVPFSIIGAVWFLYLLGYNLSIAVWVGMIAL
                     MGLDAETGIFMLLFLDLAHDDAKAKGLLRNRADLIESIIHGAVKRVRPKAMTVCAAFF
                     GLIPIMWSTGAGADLMKRIAAPMVGGLVTSFALELLVYPAVYMLWRERELPRALPA"
     gene            5398..>6005
                     /locus_tag="PLB01_15795"
     CDS             5398..>6005
                     /locus_tag="PLB01_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78817.1"
                     /translation="MIPRRRNLARAVRVLGVLFVLTGCAGLLAEQCFEKMLTGLVGAS
                     TPAAAIVLSVYFLGLTLGSLTYGWWLSGRWPPLRAYAMLEGGVAIWALLLALAAGRGV
                     EWFVPLLRLGADGFWSLQALRFVVACVWILPPTFLMGASFPAIVNALEEMRVPQPRRA
                     MSRFYALNLAGAFLGALLGPYVFFPALGLRGTLLLTCGVDAAA"
BASE COUNT          894 a         2078 c         2056 g          977 t
ORIGIN      
        1 cgagcaggtc gcgacgtggc gccagatgga cgagacgatc cgcgcccgtt acgcggccgg
       61 gatggggacg cagcaggacg tgatccgcgc ccagagcgag caaacgcggc tcctccagca
      121 gaggcggcgc gaccaggccg ccgaggaagg cgcgctggcg gagctgcggc gcctcctcga
      181 tttctctccg gaggcgcccg tggccgccgc cgcgcggctt tcccctggag tctcgctcgc
      241 cgttccgggc cgcgacgaag cggtcgcgaa ggccctcggc gcgacgcccg agatccggga
      301 cgccgcggcg ctcggcgagc gctcgcgctt cgcggcggac ctcgccaaac gggggactca
      361 gccggacttc gtcgtctcgg ctgcgtacat gaaccgcggc tcgctgccgc tcatgtgggc
      421 cgcgagcgtc ggcgtgtcgg tgccgatctt cgcgggcgcc cgcacgcggc cgctcgtcgc
      481 ggaggcccag gggctctccg actcggcggc ccgggcggag gcggccctga aatcgatcgc
      541 gcgcgcccgc gcggaggagc gcctcatccg gatgcgccag ctcgcggacg aggcgcggct
      601 ggacgccgac ggcgtcctcg tccaggaccg tctctcggtg gacgccgcgc tcgcgagcta
      661 ccgctccgga gccgtcccgt tcgtgacggt cctcgaggcc ctcggcacgt tctaccagga
      721 ccggcgcgcc gcggtcggga ggatcgcggg ctttcttcgc gcggacgcgg acctccgcga
      781 gtacgcgctc gaccgaaccg ccgcgacggc gggttcatcg acgccctcgt cctcgacgag
      841 cggcgccggg atgtagggag accaggatga atcaccttcg aagaaatctt cactccctcc
      901 cggccttggc cgccgtggcc gcgctgcttc tcttcgcgcc tgcgtgcggg agcaagaaac
      961 cctcctccgg cggcggcgcc gccgcgaagg cggcgaagtg gcagtgcccg atgcatcccg
     1021 aggtcgtcaa ggacggtccg ggcgactgcc cgatctgcgg catgaagctc gttcccatcg
     1081 aggaggccgc ccccgcagcg ccgaagagcg cgggcgccgg gcccgagggc ctcgcgacgg
     1141 tcacgatcga cgcggcaaag cgtcagctcc tcggactgaa gacggtggcc gtgacgcgcg
     1201 ccccgttcgc cacgtccatc cggacgaccg ggcggatcgc ggcggacgag aggcgcgtgc
     1261 atcacgtcca tacgcgctac gaaggcttcg tcgagcacgt caccgcggac ttcacgggca
     1321 agtacgtgca ccggggcgag gtcctcgcgc tgatctacag cccggagctc tacgccacgc
     1381 agcaggagta cctgctcgcg ctcaaggcgt cgcgctcgct gggaccctcc ggcgtgccct
     1441 ccgttgcgca gggcgggcgc gacctcctcg aggccgcccg ccagcgcctc ctcctctggg
     1501 agatcacgcc cgccgacatc gcggcgatcg agaagaaggg cgagccgatc cgcgcgatgc
     1561 ccgtctacgc gccgatctcc ggatacgtca cgggacgcac cgcgtaccac gggatgaagg
     1621 tgacgtcggc cgacacgctg ttcgacatcc tcgacctgtc ggcagtgtgg gtgctcgcgg
     1681 acgtctacga gtacgagctg ccgcgcctcg cggcgggcga gaaggcgacg atgacgctct
     1741 cgtactggcc gggccgctcc tggcagggca aagtcacgta cgtctacccg tccgtggacg
     1801 agaagacgcg caccgtgaag gtgcgcgcgg agttcgacaa cccgaagggc gagctgaagc
     1861 cggagatgtt cgcggacgtc acgcttcacg ccaagccgcg ctcggtcctc caggtgccgg
     1921 acgacgccgt tctcgagagc ggcacgcgga acatcgtctt cgtgaccgag ggcgaggggg
     1981 ctctcgcccc gcgcgagatc gcgctcggcg atcgcggcga ggggatggtg gaagtccgga
     2041 gcggactcaa ggagggcgac gtcgtcgtcc ggggcgccaa cttcctcgtg gattccgagt
     2101 cgcgcctgaa ggccgcgatc tcggcgatgg gaacgtcgag atgatccggc ggatcatccg
     2161 gttctcggcc gagaacaaat acctcgtcat cgcgctctac gcgatcgtca tcgtcctcgc
     2221 gatcggcgtc atcaggagga cgccgctgga cgccatcccc gacctctcgg acacgcaggt
     2281 catcgtgtac tcgcgctggg accgcagccc ggacgtcctc gaggaccagg tcacgtaccc
     2341 gatcgtggcc tcgctcctcg gctcggcgaa ggtcaagacg atccgcgggt tctccgattt
     2401 cggcttctcg tacgtctacg tgatcttcga ggacggcacg gacatctact gggcgcgctc
     2461 ccgcgtcctc gagaagctct cgacgatcca gtcgcgcctg cccgcgggcg tcaagaccga
     2521 gctcgggccg gacgcgacga gcgtcggctg gatctaccag tacgcgctcg tcgacgagag
     2581 cgggcggaac gcgaccgacg acatccgctc ctaccaggac tggtttctgc gatacgccgt
     2641 gcagtccgtc cccggggtcg cggaggtcgc gagcgtcggg ggcttccaga agcaatacca
     2701 ggtcacgctg gacccgaaca cgctttcggc ctatggaatc ccgctcgagt ccgtcgtgga
     2761 ggcgatccgg aagagcaaca acgagatcgg cgggcgcctc atcgagttct cgggccgcga
     2821 gtacatggtg cggggccgcg gctacctcaa gacggtcgag gacatcggca ggatcgtcgt
     2881 caagacgaac gagaagggga cgccgctgac cgtcgcggac gtgggccgcg tcgcgctcgg
     2941 ccccgagatc cggcggggcg tgaccgatct cgacggcaag ggggatgccg tcggcggcat
     3001 cgtcgtgatg cgcagcgggg agaacgcact cgacgtcatc gaccgcgtga aggctcgcct
     3061 gaaggagctc gagccgtcgc ttccgccggg actgaagatc gtcacgacgt acgaccggtc
     3121 ggacctcatc aagcgatccg tcgacacgct ccggtcgaag ctgatcgagg agatcatcgt
     3181 cgtctcgctc gtcatcctcg tcttcctctg gcacgtgccg agcgcgatcg tcccgatcgt
     3241 gacgatcccg atctcgatcg tcctcgcgtt catcccgttc aacgcgatgg gcctgaacat
     3301 caacatcatg tcgctcgggg gcatcgcgat ctcgatcggc atcctcgtgg acggcgcgat
     3361 cgtcgaggtg gagaacgcct acaagaagct ccaggagtgg aacgacggcg gccggcccgg
     3421 ggacttccac aaggtgcgcc tcgaggcgct catggaggtc gggccgtcgg tgttcttctc
     3481 gctcctcgtc gtcgccgtcg ccttcctccc ggtcttcacg ctcgtcgacc aggaagggcg
     3541 cctcttcaag ccgctcgcgt tctcgaagaa cctcgcgatg gcgatcgcgg cgttcctcgc
     3601 gatcaccctc gacccggcga tccggatgct cttcacgcgc atggacggct tctcgttccg
     3661 gccgagatgg ctggcctgga tcgtcaacca cgccgtcgtg gggaagtact acgccgagga
     3721 aaggcacccg atcagcgtcg ttctccaccg gctctacgag cggccctgcc gggccgtgct
     3781 ccggcacccg agaaagacga tcgcgatcgc cctcctcgcg ttgctcgcca cggtccccgt
     3841 cttcctgaag ctcgggtccg agttcatgcc gccgctctac gagggatcga tcctctacat
     3901 gccgacgacc ctgcccggaa tctccgtgac cgaggccgag cggctgctcc agacgcagga
     3961 ccgggttctc aagtcctttc ccgaggtcga gcgcgtcttc ggcaaggcgg gccgcgccga
     4021 gacgtccacg gacccggctc cgttctcgat gatggagacg acgatcatcc tcaagcccga
     4081 gtccgagtgg cgcggcaagg accggtggta ctcgtcgtgg gcgcccggct ggctggcggg
     4141 cgtcttccgg cacgtctggc gggaccgcat cacccacgac gagctcgtcg acgagatgga
     4201 ccgcgccctg aagatcccgg ggacgacgaa cgcctggacg atgccgatca aggcgcggat
     4261 cgacatgctc tcgaccggca tccggacgcc ggtcgggatc aagatcttcg gctcgagcct
     4321 cggagagatc gagcggatcg gcgccgaggt cgaggcgacg gtgaagagcg tctccggcac
     4381 gcgttccgcg ttcgccgagc gcgtggccgg cggctacttc gtggacttcg acctgaaccg
     4441 ggacgcgctc gcgcggtacg gcctcacggt ggacgacgcg aacgcggtca tcatgacggc
     4501 gatcgggggc gagaccgtca cgacggtgat caagggccgc gagcggtact ccgtcaacgt
     4561 gcgttactcg cgcgagctgc gcgaggactt cgaccggctc gcgcgcgtcc tcgtcccgac
     4621 gatgggaggc gcccaggtgc ctctcggcca gctcgcgacg atccgccagg ttcgcgggcc
     4681 ggcgatgatc cggaacgaga acgggctcct ctcgggctac gtctacgtcg acttcgacac
     4741 gtcgaaggag gacgtcggcg ggtacgtcga acgggcgaag aaggccgtcg ccgcgcacgt
     4801 gacgcttccg ccggggtacg cgctcgtctg gagcggccag tacgagaaca tgctgcgcgt
     4861 caaggagcgg ctcaaggtcg tcatcccggt cacgctcctc ctgatctggc tcctcatgta
     4921 cgcaaacacg aagtcggcga tgaagacgat gatcgtcctc ctcgccgtgc cgttctcgat
     4981 catcggcgcc gtctggttcc tgtacctcct cggctacaac ctctcgatcg ccgtgtgggt
     5041 cggcatgatc gcgctcatgg gcctcgacgc ggagacgggg atcttcatgc tcctcttcct
     5101 cgacctcgcg cacgacgacg cgaaggcgaa ggggctcctg cggaaccgcg cggacctgat
     5161 cgagtcgatc atccacgggg ccgtcaagcg cgtgaggccg aaggccatga cggtctgcgc
     5221 cgcgttcttc ggcctgatcc cgatcatgtg gtcgaccggt gccggcgccg acctcatgaa
     5281 acgcatcgcc gcgccgatgg tgggcggtct cgtgacgtcg ttcgcgctcg agctcctcgt
     5341 ctacccggct gtctatatgc tctggaggga aagggaactg ccgcgagcgc tgccggcttg
     5401 attccgcgcc gacgaaatct ggcccgcgcc gtccgggtcc tgggcgtcct cttcgtgctg
     5461 accggctgcg cggggcttct cgccgagcag tgcttcgaga agatgttgac cggtctcgtc
     5521 ggcgcgagca cgcccgcggc ggcgatcgtc ctgtccgtct actttctcgg cctcacgctg
     5581 ggcagcctga cctacggttg gtggctgtcc ggccgctggc cgcctctccg tgcctacgcg
     5641 atgctcgagg gaggcgtggc gatctgggcg ctgctcctcg ccctcgcggc cggtcgcggc
     5701 gtcgagtggt tcgtgccgct gctccgcctc ggagccgacg ggttctggtc ccttcaggcc
     5761 ctgcgcttcg tcgtggcgtg cgtctggatc ctgccgccca cgttcctgat gggcgcgagc
     5821 tttccggcga tcgtcaacgc cctggaggag atgcgcgttc ctcagccgcg ccgcgcgatg
     5881 tcgcgtttct acgcgctcaa cctggcgggt gctttcctcg gtgcgcttct cggcccgtac
     5941 gtgttctttc ccgccctcgg gctccggggg actctcctgc tgacgtgcgg cgtggacgcc
     6001 gccgc
//
LOCUS       DAOMFY010000246         5998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4446_length_5998_cov_6.555612, whole genome shotgun sequence.
ACCESSION   DAOMFY010000246 DAOMFY010000000
VERSION     DAOMFY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4446_length_5998_cov_6.555612"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLB01_15800"
     CDS             <1..1078
                     /locus_tag="PLB01_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQQ78818.1"
                     /translation="RLPDDAYAPLGRVHPRVSRVGFGCYRVDDREDVHRRALSDALAA
                     GVTLIDTSTNYADGHSETLVGAVLADSALRGAVRRADIVVVTKIGYLQGRNLDEARRR
                     VRAGRPWSEVVEYGPDLWHAISPDFLEDQLTASLERLAMPRVDLLLLHNPEYFLTHAM
                     HAGVARDEARAEFYDRTARAFRRLESEVSAGRIGAYGVSSNTLVVARERADAVSLERL
                     LAGAGPGFAAIQLPFNPIETGAREPIHTEDGRSVLDVARAAGLGVLVNRPFNAFSGRG
                     LVRFADLPPEALAEIGRDAAWEKSRAAELQAWVTSAFAGAAPAAGSLAQRTLGALLAT
                     PGVDVVLAGMRRPEYVEDVVGAFA"
     gene            complement(1075..2514)
                     /locus_tag="PLB01_15805"
     CDS             complement(1075..2514)
                     /locus_tag="PLB01_15805"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HQQ78819.1"
                     /translation="MLIHGAIAPAAAFLALFAAGPAAPAESPAAGFWNAWPAARVYTG
                     DPFALKSAALRAEIARLVDRYPGVVRVAEEGVSAEGRPIPLLLVGEGPTTVLLWSQMH
                     GDEPTATAALLDVLNHIGATRAVPATKALLSELTLAVIPMLNPDGAERTRRTNAQGID
                     INRDAGRLQTPEGRFLKSVRDRRSPAVGYNLHNQGPNQLAGAAGAQAAISLLAVPFDE
                     AFTENEGRRTTKRLAVLVRDSLKPWAAGRVSRYDMEYTARAFGDSMTRWGTPTLLIES
                     GGFAAAGADEAAALVRLNFVAILATLSALADGSVGKVDAAAYDAIPLNVRDRLMDVVV
                     RNALVIGGGGLAPFLADVGWNVPVTRPGFTGSAVGRARGGAVLEVGDLDSYKGKTEVD
                     ATNRVLVVAPKGGADGWAKALEGLKARGLATADGTLTLTLDVLSQEAKAWLDGAPALA
                     PGYGGDFLLIAPSGEGRFRVDRRLSVAGP"
     gene            2548..5163
                     /locus_tag="PLB01_15810"
     CDS             2548..5163
                     /locus_tag="PLB01_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ78820.1"
                     /translation="MTLEPGSRLGPFEVLSRLGAGGMGEVWRARDTRLQRDVAIKVLP
                     EAFAQDADRLLRFTREAHVLASLNHPNIAAIYSLEEIDGVRFLVLELVTGETLKQRIV
                     RAPVSTNETIRFALQIADALEAAHAKGVLHRDLKPANVNVTPEGKIKLLDFGLAKAFA
                     LGAASPEISHSPTLAVDPTHQGVVLGTASYMSPEQARGRALDGRTDLWSFGCVLYEML
                     AGKKAFDGESVSDILVAILDREPDWGALPAATPAPLRELLKRLLQKDPAGRLATIGET
                     RGFLEAAAGSRTTAIFPVSGRSASFARRGGGRTLLVAGAVLLAVGAGFLWLAVRERAG
                     SALPASKLLAILPATDLTGRPDGRQLCDGVSFSLGVKLRELSPDFMLIRPDAPAELKE
                     READPSKWAREKGANIIVQPVVRQLGEIREISFSIARAGSPVQIDAGEVRGAAAEHFK
                     LEDDLARKLVAALKLHLAPEARTSPRSTIPAGPSQTDYVVALGYLERYDDPASLQKAI
                     DLLKTIPGGENTATVQATLGRAYLAVYQNTHDIAFSRLAEASADRAQNLDPGRLETLL
                     TLGRIKAAAGRLDDGIVDFRRALERDPSSVEALLSLGDALADSGKPADAEAAYRRAAA
                     ARPSYWAPHNRLGALFYSKGDYGRAIPEYEEAARLNPSTPRPLSNLVGVYLQSGRLDD
                     AVRTAQKSLDLSQSASGWANLGTAHYYAGRFSDAAAAFEKATAAAPKRSSLWIGLGDA
                     LRSSGGEATRTRAAYQEALRRTDDELSVNPRSASALRNRARALRNLGEEAKAREAILG
                     ARAAAPDDSSILVEASRLALLHQDRAAAFALLSDAVRKGFSPALLRADPEFTEVRADP
                     LFEKALATPRSPGAH"
     gene            5216..5680
                     /locus_tag="PLB01_15815"
     CDS             5216..5680
                     /locus_tag="PLB01_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78821.1"
                     /translation="MGLAAIVLLAAACANGGTPPPKPAPPAPAPTGQGKTLDCKRRDP
                     EHVVLIREGQGTQDPKKKKGVAYPYRLIVGSYDPAAPHSTIIWSFRNQSTEITFERPE
                     VGVPDCKKVKYECELRLPKNLSFETQYKYTISGTDSEGKPFEDNDPFIEVDR"
     gene            complement(5690..>5998)
                     /locus_tag="PLB01_15820"
     CDS             complement(5690..>5998)
                     /locus_tag="PLB01_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78822.1"
                     /translation="PHGSTVPRTTPPPLEKRVTPAPSHSTPAGSPGAAGNTLYMTDED
                     LRALEAELAKHVGPLARVLVKKAVKGSGNMSHVITKLELEIDSDDARRAFRAAVKKMR
                     "
BASE COUNT          857 a         2210 c         2076 g          855 t
ORIGIN      
        1 acgtcttccg gacgacgcgt acgcccccct cggccgcgtc cacccgcgcg tctcgcgcgt
       61 cgggttcggg tgctaccgcg tggacgaccg cgaggacgtc caccgccggg cgctctcgga
      121 cgcgctcgcg gcgggcgtca cgctgatcga cacgtcgacg aactacgccg acggccacag
      181 cgagacgctc gtcggcgcgg tcctcgcgga ctccgcgctc cgcggcgccg tccggcgcgc
      241 ggacatcgtc gtcgtcacga agatcggcta cctgcagggc cgcaacctcg acgaggcgcg
      301 ccgccgcgtc cgcgcggggc ggccgtggag cgaggtcgtc gagtacgggc ccgatctctg
      361 gcacgcgatc tcgcccgact tcctcgagga ccagctcacg gcctcgctcg agcgtctcgc
      421 gatgccgcgc gtggaccttc tcctcctcca caatcccgag tacttcctca cgcacgcgat
      481 gcacgcgggc gtcgcacggg acgaggcgcg ggccgagttc tacgaccgga ccgcgcgggc
      541 gttccgccgc ctcgaatccg aggtctccgc cgggcgcatc ggggcgtacg gagtctcctc
      601 gaacacgctc gtcgtcgcgc gcgagcgggc ggacgcggtc tcgctcgagc ggctcctcgc
      661 aggcgccggg ccgggcttcg ccgcgattca gcttccgttc aacccgatcg agacgggcgc
      721 ccgcgagccg atccacacgg aggacggccg gagcgtcctc gacgtcgcgc gcgccgccgg
      781 cctcggcgtc ctcgtcaacc ggccgttcaa cgcgttctcg ggccgcgggc tcgtccgctt
      841 cgcggacctt ccgccggagg cgctcgcgga gatcggccgc gacgcggcct gggagaagtc
      901 gcgagccgcc gagctccagg cgtgggtcac gtcggccttc gcgggcgcgg cgcccgccgc
      961 gggctcgctc gcccagcgca cgctgggcgc cctcctcgcg acgccgggcg tcgacgtcgt
     1021 cctcgccggc atgcgccggc ccgaatacgt cgaggacgtc gtcggcgcct tcgcctaggg
     1081 acccgcgacc gagagccgcc ggtccacgcg gaagcggcct tcgccggacg gtgcgatgag
     1141 gaggaagtcg ccgccgtagc cgggagcgag cgccggggcg ccgtcgagcc acgccttcgc
     1201 ctcctgcgag agcacgtcga gcgtgagcgt gagcgtcccg tcggccgtcg cgaggcccct
     1261 cgccttcagc ccctcgagcg ccttggccca cccgtcggcg ccccccttcg gcgcgacgac
     1321 gaggacgcgg ttcgtcgcgt cgacctcggt cttgcccttg tacgagtcga ggtcgcccac
     1381 ttcgagcacc gcgccgcccc gggcgcggcc gacggcggag cccgtgaatc ccggccgcgt
     1441 gacgggaacg ttccagccga cgtccgcgag gaagggcgcg agaccgccgc ccccgatgac
     1501 gagtgcgttc cggacgacga cgtccatgag ccggtcgcgc acgttcagcg ggatcgcgtc
     1561 gtaggccgcc gcgtccacct ttccgacgga gccgtccgcg agggccgaaa gcgtcgcgag
     1621 gatcgcgacg aagttgaggc gcacgagcgc cgcggcctcg tcggccccgg cggccgcgaa
     1681 gccgcccgac tcgatcagga gcgtcggcgt cccccagcgc gtcatcgagt cgccgaacgc
     1741 ccgcgccgtg tactccatgt cgtagcgcga gacgcggccc gccgcccagg gcttcaggct
     1801 gtcgcggacg aggaccgcga gccgcttcgt cgtgcgccgc ccctcgttct ccgtgaacgc
     1861 ctcgtcgaag ggcaccgcga ggagcgagat cgcggcctgc gcgccggcgg cgcccgcgag
     1921 ctgattcggg ccctggttgt ggaggttgta gccgaccgcc gggctgcgcc tgtcgcgcac
     1981 ggacttgagg aaccgccctt cgggcgtctg gaggcggccc gcgtcgcgat tgatgtcgat
     2041 gccctgcgcg ttcgtccggc gggtgcgttc ggccccgtcg ggattgagca tcggaatgac
     2101 cgcgagcgtc agctccgaga gaagcgcctt cgtcgcgggc accgcgcgcg tggcgccgat
     2161 gtggttcagg acgtcgagga gagcggccgt ggccgtcggc tcgtccccgt gcatctgcga
     2221 ccagagcaga accgtcgtcg gcccctcgcc gacgaggagg agcggaatgg gacggccctc
     2281 ggcggagacg ccctcctcgg ccacgcggac gacgccgggg taacggtcca cgaggcgcgc
     2341 gatctccgcc cggagtgccg ccgacttgag cgcgaaggga tcgcccgtgt aaacgcgcgc
     2401 cgccggccat gcgttccaga agcccgccgc gggtgattcg gccggtgcgg ccggacccgc
     2461 ggcgaacagg gcgaggaagg cggcggcggg cgcgatcgca ccgtgaatca acatagggtc
     2521 cggattgtag ataatcgtca cgacaccatg actctcgaac ccggttcccg cctcgggccg
     2581 ttcgaggttc tgtcgcgcct cggcgcggga gggatgggcg aggtgtggag ggcgcgcgac
     2641 acgcgcctgc agcgcgacgt cgcgatcaag gtccttccgg aagcgttcgc acaggacgcc
     2701 gaccgcctcc tgcgcttcac gcgcgaagcc cacgtcctcg cgtccctgaa ccacccgaac
     2761 atcgcggcga tctacagcct cgaggagatc gacggcgtcc gcttcctcgt cctcgagctc
     2821 gtgaccggcg agacgctcaa gcagcgcatc gtgcgcgcac ccgtctccac gaacgagacg
     2881 atccggttcg cgctccagat cgcagacgct ctcgaggccg cgcacgccaa gggcgtcttg
     2941 caccgcgacc tgaaacccgc gaacgtcaac gtgacgcccg agggcaagat caagctcctc
     3001 gacttcggcc tcgcgaaggc cttcgccctc ggcgcggctt cgcccgagat ctcgcactcg
     3061 ccgacgctcg cggtcgaccc gactcaccag ggcgtcgtcc tcgggacggc gagctacatg
     3121 agccccgagc aggcgcgcgg ccgggctctg gacggccgca cggacctctg gtccttcggc
     3181 tgcgtgctct acgagatgct cgcgggaaag aaagcgttcg acggcgagag cgtgtcggac
     3241 atcctcgtcg cgatcctcga ccgcgagccg gactggggcg cgctgccggc cgcgacgccg
     3301 gcgccgcttc gagagctcct gaagcggctg ctccagaaag acccggccgg gcgcctcgcg
     3361 acgatcggcg aaacgcgggg tttcctcgag gctgcggcgg gctcccgcac cacggccatc
     3421 ttccccgtct cggggcgatc cgcgtccttc gcgcgccgcg gaggcggccg gaccctcctc
     3481 gtcgccgggg ccgtgcttct cgccgtcggc gccgggttcc tctggctggc cgtgcgcgag
     3541 cgggccggct cggccctgcc cgcctcgaag ctgctcgcga tcctgcccgc caccgacctc
     3601 accgggcgcc cggacgggcg ccagctctgc gacggcgtgt ccttcagcct gggcgtgaag
     3661 ctccgggagc tctctccgga cttcatgctg atccggccgg acgctccggc agagctgaag
     3721 gagagagagg cggacccctc gaagtgggcc cgcgagaagg gcgcgaacat catcgtccag
     3781 cccgtcgtcc gtcagctggg agagattcgc gagatctcgt tctcgatcgc gcgggccggc
     3841 tctcccgtac agatcgatgc cggcgaggtc aggggcgcgg ccgcggagca tttcaagctc
     3901 gaagacgatc tcgcccggaa gctcgtggcc gcactcaagc ttcacctcgc gccggaagcg
     3961 aggacctcgc cccgctccac gatccccgcc ggcccgtcgc agacggatta cgtcgtcgcg
     4021 ctcgggtacc tcgagcgtta cgacgatccc gcgtcgctcc agaaggcgat cgacctcctg
     4081 aagacgatcc cgggcggaga gaacaccgcg accgttcagg cgactctcgg ccgcgcctat
     4141 ctcgccgtgt accagaacac gcacgacatc gccttctcgc ggctcgccga ggcgagcgcc
     4201 gatcgcgctc agaacctgga ccccggccgg ctggagacgc tcctgacgct cggccggatc
     4261 aaggcggcgg cgggacgcct cgacgacgga atcgtggact tccggcgcgc gctcgaacgc
     4321 gatccgtcat ccgtggaagc gctcctttcg ctgggcgatg cgctggcgga ctcggggaaa
     4381 cccgccgacg cggaggccgc gtaccggcgg gccgccgccg ctcgtccgtc ctactgggct
     4441 ccgcacaatc ggctcggtgc gctcttctac tcgaaaggcg actacggccg cgcgatcccg
     4501 gagtacgaag aggcggcgcg cctgaatccc tcgacgcctc gtccgctctc caacctggtc
     4561 ggggtctacc tccagagcgg caggctggac gacgcggtcc gtacggcgca gaagtcgctg
     4621 gatctctcgc agagcgccag cggctgggcg aacctcggaa ccgcccacta ttacgcgggt
     4681 cgtttctcgg acgcggccgc cgccttcgag aaggcgaccg cggccgcgcc gaagcgctct
     4741 tccctgtgga tcggcctcgg cgacgcgctc cggtcgtccg gcggcgaggc gacccgcacg
     4801 cgcgccgcct accaggaagc cctgcgccgg accgacgacg agctgagcgt caacccccgg
     4861 tccgcgtccg cgctgcgcaa ccgggcacgt gcccttcgaa acctgggcga agaggcgaag
     4921 gcgcgcgagg cgattctcgg ggcgcgcgcc gcggcgcccg acgactcgtc gatcctcgtc
     4981 gaggcctcgc gcctcgccct cctgcaccag gaccgcgccg ccgccttcgc cctcctgtcc
     5041 gatgccgtgc gcaaggggtt cagtccggcc ctcctgcggg ccgatcccga gttcaccgag
     5101 gtcagggccg atccgctctt cgagaaggca ctcgcgacac cgcgctctcc gggagcgcat
     5161 tgaacgaagc tcaaggaggc tctctatgtc cgtgcattct tcatggcgcg ttcagatggg
     5221 gctcgccgcg atcgtcctgc tcgccgccgc ctgcgccaac ggcggaacgc cgccgccgaa
     5281 gccggcccct ccggcgcccg cgccgaccgg ccaaggcaag acccttgatt gtaagaggcg
     5341 agatccggag cacgtggttc tgatccggga gggtcagggg acccaagatc ccaagaagaa
     5401 gaagggggtc gcctatccgt accgactcat tgtcggaagc tacgacccgg ctgctccaca
     5461 ttccacgatc atctggtcgt tccgtaacca gtcgacggaa atcaccttcg agaggccgga
     5521 ggtcggagtt cccgactgca aaaaggtcaa gtatgagtgc gagttgcggc tcccgaagaa
     5581 cttgagtttc gagactcagt acaagtacac gatctcgggt accgacagcg aaggcaagcc
     5641 cttcgaggac aacgaccctt tcatcgaggt cgaccgctag cgtccgcggc taccgcatct
     5701 tcttgacggc cgcgcggaag gcgcggcggg cgtcgtcgga gtcgatctcg agctcgagct
     5761 tcgtgatcac gtgcgacatg ttgcccgagc ccttcacggc cttcttcacg aggacgcgcg
     5821 cgagcggacc gacgtgcttc gcgagctcgg cctcgagggc gcgcaggtcc tcgtccgtca
     5881 tgtacagcgt gttccccgcc gcgccgggcg agccggcggg cgtggagtgc gagggcgccg
     5941 gcgtcaccct cttctcgagc ggcggcggcg tcgtgcgcgg caccgtggag ccgtgcgg
//
LOCUS       DAOMFY010000247         5883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4590_length_5883_cov_6.560913, whole genome shotgun sequence.
ACCESSION   DAOMFY010000247 DAOMFY010000000
VERSION     DAOMFY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4590_length_5883_cov_6.560913"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..344)
                     /locus_tag="PLB01_15825"
     CDS             complement(72..344)
                     /locus_tag="PLB01_15825"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HQQ78823.1"
                     /translation="MSQKCVEVIFGKLATDEELRRRFQADPLGTLGQLAEHGFELTRA
                     ECEALAATRSDLFTRLSEAIDPRLQKASLRPDPPVPAPSSGERRPS"
     gene            complement(601..894)
                     /locus_tag="PLB01_15830"
     CDS             complement(601..894)
                     /locus_tag="PLB01_15830"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQQ78824.1"
                     /translation="MARPPLLSENDVTARLAAHPAWTYSGGRLHREFRFAGFSEAFGF
                     MARVALFAEKLDHHPDWSNVWNRVVVSLHTHDAGGVTDLDFRLAAEMDRIAGG"
     gene            960..2282
                     /locus_tag="PLB01_15835"
     CDS             960..2282
                     /locus_tag="PLB01_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78825.1"
                     /translation="MRLLKALALTALLAAGAGLVWFAGTAAWAARRESQALGAWNALG
                     LPLSEAAPFPKKETNAAALALEKAAEPLGIHLKPGGGAAEVEPEPVGMVREEKTEWGR
                     VRGALTKWATNEAERPEANVQAPPPEVAAWLTGQAAVLDAIEGALVGGPAPEWASDSS
                     LLFAAPAPGMSGHLQLHVVLLGRALARAAGGDAAGAERSLLASWNFAAPQRLRADVPS
                     RSIAALATHLELGVLRKLAVDPGPWRARIAGWDPRDAVKRTWANEAWTTWQAGRRQVP
                     APAQGSFARLVGRPGKRLEAAAFLDGWRSMTEAGVKSPVSDGDGREIAAAFRAGMGRW
                     ADAAPAIPNLASAWKRADRLILEAELTNKVFDVRAARDASGAWPASVKGIEASKAKDL
                     SWTYAVTSEGRASIATNRTLTWPDRASSPLGWVSEPPAASKAPAPKRR"
     gene            2292..3419
                     /gene="mnmA"
                     /locus_tag="PLB01_15840"
     CDS             2292..3419
                     /gene="mnmA"
                     /locus_tag="PLB01_15840"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQQ78826.1"
                     /translation="MRIAVAMSGGVDSSVAALLLKREGAELVGLSMHLWDHDRDGTGS
                     AAGRCCTLDDLTVARRAAEAIGIPHYVLDLTERFEEAVVRPFVTSYLEGRTPIPCTAC
                     NTEVKFKTLLARAEALGCDAVATGHYARVETDAVTGERRLLKARDLGRDQSYFLYDLT
                     SEQLARVRFPLGELEKTAVREMAREAGLPNWDKPDSQEICFVPNGTTPADFIRKEAAN
                     LGIPLPALAGARPGKIFLEGGESVGAHEGTFGYTIGQRRGLGISSNDPLYVLSIQPDE
                     GALVVGPAASLLSKKILLEAVTLRSPRRHAGAISVLARIRSRHPEQPAALTLEDGGRA
                     FLEFEEPVRAAAPGQSAVFYDPARPDLVLGGGTISRPTPPA"
     gene            3511..4227
                     /locus_tag="PLB01_15845"
     CDS             3511..4227
                     /locus_tag="PLB01_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78827.1"
                     /translation="MVPRTKLSIDAADLRDLIVPGLGNRRIFRILLADACRHACDDCP
                     MRAERDLPPHALDAPRLARLFMTAFRRGWCDGLFITSGIPKDAVRAMDRMLEVVELLR
                     VAHGYRGYLHVKAVAGAEAGQIERLVRLVDRVSYNLEVRCRRAQEEDATVPGSGKQSE
                     VPVFELAAAAAVREARRDSARKAPPELPPVAVDRGKRATAFAAWRGLMVRPRPAQASL
                     FPAASSVLSRPWGVAPGTFR"
     gene            4312..5106
                     /locus_tag="PLB01_15850"
     CDS             4312..5106
                     /locus_tag="PLB01_15850"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HQQ78828.1"
                     /translation="MAKSAAAAASILKDVRALLEARADEKETAKDGKANGRSKGGVLQ
                     PRSPLKALGVKPSFVRSIAREIQTKNRPAMDYTAAVALMDAAVSRKVREEILVALDVL
                     ERHRKDFAAGLFSHVEKWSLVADDLEVAEALGTRVAAPALGLEPAKINVLRKWARLRS
                     VGKRRLAVLAAVGLVSDGRREAPAVLELAELLLHEEHPAMVSAVASLLRETTKVDAKA
                     VQDFLFRRSVDGNPDILRAGSENLDAARRAALIAKLEAQAALVATT"
     gene            complement(5224..>5883)
                     /locus_tag="PLB01_15855"
     CDS             complement(5224..>5883)
                     /locus_tag="PLB01_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78829.1"
                     /translation="FYDHFNHHPARPGPPASPKVRPAPPERPPGIVAVGSTPISLDYA
                     PLTNEEKAQAAALHQFDFLNTFETLARLDAFFPEPVGPRAPVPVPPGGFPSRPPDTPD
                     GLTGRFLDWQRASAKERARLVALESSFRNRPEDERALLEDRWTLVKDFTEEQQVGLRR
                     LASRLDEVDARSHDRLESEIRLIGRLPMSQRVARWRGLPFAKPLTGQELESAERLLLS
                     R"
BASE COUNT          842 a         2020 c         2111 g          910 t
ORIGIN      
        1 cgcgggcgcg ggggcgggct gagccgcgag cgtcgccgtg ccgaggagaa gggcggcgag
       61 agccgtacgg atcacgaggg tctcctttct ccggaggacg gagcgggaac gggcgggtcc
      121 ggacgcaggc tggccttctg gagccgcggg tcgatcgcct cggagaggcg ggtgaacagg
      181 tcggacctgg tggcggcgag cgcctcgcat tcggcgcgcg tcagctcgaa gccgtgctcg
      241 gccagctgac cgagggtccc gagcgggtcc gcctgaaagc ggcgcctgag ttcctcgtcg
      301 gtggcgagct ttccgaaaat cacctcgacg cacttttgcg acatggcggc agtatctccc
      361 gcattgtcgc tttacatacg ccgtgccatg cgccctgggt tttgttcgaa gagaagataa
      421 agatttctta gaaggtggtt taggcgattg ctgcggcggc gcgcggcggg agccgcccac
      481 cttggggtaa tgccgggaaa tcgacacatt gaggcgggcc gcccgcacga gcctcggccc
      541 cgcccctctt caccttctaa gaaatccccc gggtcttcct cttcctcttc ctctttctct
      601 tcagccgccc gcgatccgat ccatctccgc cgcgaggcgg aagtcgaggt cggtgacgcc
      661 gccggcatcg tgcgtgtgga gggagacgac gacgcggttc cacacgttcg accagtcggg
      721 atggtggtcg agcttctcgg cgaagagcgc gacgcgcgcc atgaagccga acgcttccga
      781 gaatcccgcg aaacggaact cgcggtggag tcttccgccc gagtaggtcc atgcgggatg
      841 cgcggcgagt cgggccgtca cgtcgttctc ggagaggagg ggagggcgtg ccatggcgcg
      901 attctgcccg atggggccgc ggcgccggga cctttatgat cgggggttcg gaggtcccga
      961 tgagactctt gaaagccctc gcgttgaccg ccctcctcgc cgccggcgcc ggcctcgtct
     1021 ggttcgccgg aactgcggcc tgggcggcgc ggcgcgagtc gcaggcgctc ggcgcgtgga
     1081 acgccctcgg acttcccctg agcgaggcgg cgccgttccc gaagaaggaa acgaacgccg
     1141 cggccctcgc gctcgagaag gccgccgagc cgctcgggat ccacctcaag cccggcggcg
     1201 gcgcagcgga ggtcgagccg gagcccgtcg gaatggtccg ggaagagaag acggagtggg
     1261 gccgcgtgcg cggcgccctc acgaagtggg cgacgaacga ggcggagcgg cccgaggcga
     1321 acgtccaggc gcctccgccc gaggtggcgg cctggctgac gggccaggcg gccgtgctcg
     1381 acgccatcga gggcgcgctc gtcggcggtc ccgcacccga gtgggcctcg gattccagcc
     1441 tccttttcgc ggctcccgca cccggaatgt ccggtcacct ccagctccac gtcgtgcttc
     1501 tcggacgcgc cctcgcgcgc gcggcaggcg gcgacgcggc cggagcggag cgcagcctcc
     1561 tggcttcgtg gaacttcgcg gccccccaac gcctgcgcgc cgacgtgccg agccgttcga
     1621 tcgcggcgct cgcgacgcat ctcgagctcg gcgttctgcg caagctggcc gtggatcccg
     1681 gaccgtggcg agcccggatc gccggctggg atccgcgcga cgccgtcaag cgcacgtggg
     1741 cgaacgaggc gtggacgacg tggcaggccg gccggcggca ggtgccggca ccggcgcagg
     1801 gctccttcgc gcggctcgtc gggcgccccg gaaagcgcct cgaggccgcc gcgttcctcg
     1861 acgggtggcg ctccatgacg gaagcgggag tcaagagccc ggtctcggac ggcgacggcc
     1921 gggagatcgc cgcggcgttc cgcgcgggca tgggtcgctg ggcggacgca gccccggcga
     1981 tcccgaacct cgcgagcgcc tggaagcgcg ccgaccggct catcctggag gccgagctca
     2041 cgaacaaggt gttcgacgtg cgcgccgcgc gcgacgcgtc gggcgcgtgg cccgcgtcgg
     2101 tcaaagggat cgaggcctcg aaggcgaagg acctgtcgtg gacgtacgcc gtgacgtccg
     2161 aggggcgcgc ctcgatcgcg acgaatcgca cgctgacgtg gcccgaccgt gcgtcctcgc
     2221 cgctgggctg ggtctccgag ccgcccgcgg cgtcgaaggc cccggcgccg aagcgccgat
     2281 aatcccgtcc cgtgcggatc gccgtcgcca tgtcgggagg ggtcgactcc tccgtcgcgg
     2341 cgcttctcct gaagcgcgag ggggcggagc tcgtcggcct ctcgatgcac ctgtgggacc
     2401 acgaccgcga cggcaccggc tcggcggccg gccgctgctg cacgctggac gacctcaccg
     2461 tggcgcggcg cgccgccgag gcgatcggga tcccgcacta cgtcctcgac ctcacggagc
     2521 gtttcgagga ggcggtcgtc cgacccttcg tgacgagcta cctcgaaggc cggacgccga
     2581 tcccctgcac ggcctgcaac accgaggtca agttcaagac gctcctcgca cgcgcggagg
     2641 cgctcgggtg cgacgccgtg gcgaccggcc actacgcgcg cgtcgagacg gacgctgtga
     2701 ccggagagcg gcgtcttctc aaggcccggg acctcggccg cgaccagtcg tacttcctct
     2761 acgacctcac gagtgagcag ctcgcgcgcg ttcgcttccc gctcggggag ctcgagaaga
     2821 cggccgtgcg cgagatggcc cgcgaggcgg gccttccgaa ctgggacaag ccggactcgc
     2881 aggagatctg tttcgtcccg aacgggacca cgcccgcgga cttcatccgc aaggaggccg
     2941 cgaacctcgg cattcctctg ccggctctcg cgggggcacg gcccgggaag atcttcttag
     3001 aaggtggaga aagcgtcgga gcgcacgaag ggactttcgg ctacacgatc ggccagcgcc
     3061 ggggacttgg gatttcttcg aatgatcctc tctacgtact ctcgattcag cccgatgagg
     3121 gggcgctcgt cgtggggccg gccgcctctc tactttctaa gaagatcctc cttgaggcgg
     3181 tgactctgcg cagcccgcgc cgccatgccg gggcgataag cgttcttgcc cgcatccgct
     3241 cgcgccatcc ggagcagccc gctgcgctga ccctcgagga cggcggccgc gcatttctcg
     3301 agttcgaaga gccggtccgg gccgccgccc ccggccagtc ggcggtcttc tacgacccgg
     3361 cccggcccga tctcgttctg ggcggcggaa cgatctcccg gccgactcct cccgcctgac
     3421 ctcgatttca ttgtgggttt tgtcatttct cattccggcc cttgacttgc gcgtaactta
     3481 cgcctatatt gcgtcccggt gaggtgagac atggtcccga ggacgaagct ctcgatcgac
     3541 gccgccgacc tccgggatct catcgtgccc gggctcggga accggcgcat cttccggatc
     3601 ctcctcgcgg acgcgtgccg gcacgcgtgc gacgactgcc cgatgcgcgc ggagcgcgac
     3661 cttccgccgc acgcgctcga cgcgccgcgc ctcgcgcgcc tattcatgac ggcgttccgg
     3721 cgcggctggt gcgacggcct gttcatcacg agcggcatcc cgaaggacgc cgtgcgagcg
     3781 atggacagga tgctcgaggt cgtcgagctc ctgcgtgtcg cgcacggcta ccgcggctat
     3841 ctgcacgtca aggccgttgc gggcgcagag gcggggcaga tcgagaggct cgtgcgtctc
     3901 gtcgatcgcg tgtcgtacaa cctcgaggtt cgctgccggc gcgcgcagga agaagacgcg
     3961 acggtcccgg gttccgggaa gcagtccgag gtgcccgtct tcgagctcgc cgcagccgcg
     4021 gccgtccgcg aggcgcggcg cgactcggcg cgcaaggcgc ctccggagct gccgcccgtc
     4081 gctgtagatc gcgggaaacg tgccacggcg ttcgcggcct ggcgcggcct gatggtccgg
     4141 ccgcggccgg cccaggccag cctcttccca gctgcaagta gcgtcctgtc aaggccttgg
     4201 ggagtcgctc ccgggacttt ccgctgacgc cgaagccgcc tggacgcggt ccgttgccgg
     4261 ttccgtgacc tcggaagtgg tcaccccgtg tgctagaatc agagtttgca tatggccaag
     4321 tctgccgctg ccgccgcctc gattctcaag gacgtccgag cccttctcga ggcacgcgcc
     4381 gacgagaaag agaccgcgaa ggacggcaag gctaacggaa ggtcgaaggg cggcgtcctc
     4441 cagccgcgga gcccgctgaa ggcgctcggc gtgaagccgt cgttcgtgcg ctcgatcgcg
     4501 cgcgagatcc agacgaagaa ccggcccgcg atggattaca ccgccgcagt cgccctcatg
     4561 gacgccgccg tgagccgcaa ggtgcgcgag gagatcctcg ttgcgctcga cgtcctcgag
     4621 cgccaccgga aggacttcgc ggccggtctg ttctcccacg tcgagaaatg gagtctggtg
     4681 gccgacgacc tcgaggtcgc cgaggcgctc ggaacccgcg tcgcggcgcc cgcgctcggc
     4741 ctggagcccg caaagatcaa cgtgcttcgc aagtgggcgc gcctccgcag cgtcggcaag
     4801 cggcgccttg ccgtccttgc cgccgtcggc ctcgtgagcg acgggcgccg cgaggcgccg
     4861 gccgtgctcg agctggccga gctccttctg cacgaggaac acccggcgat ggtttcggcc
     4921 gtcgcgagcc tgctccgcga gacgacgaag gtcgacgcga aggccgttca ggacttcctg
     4981 ttccgccgtt cggtggacgg caacccggac atcctccgcg ccggctccga gaatctcgat
     5041 gcggcgcgcc gcgccgcgct catcgcgaag ctcgaagccc aggctgcgct ggttgcaacg
     5101 acctgacgcc cggtccggtt tgttttcggg tcttctgtct cttttcgttt tgagagagcc
     5161 gcgcaatcgg cgctgcgccg gtgcccgagg ctcgggaacc ttctaggaat ctcttccgag
     5221 gattcagcgc gagagcagca ggcgctcggc gctctcgagc tcctgtcccg taagcggttt
     5281 cgcgaacggg agtccgcgcc agcgcgccac gcgctgggac atcgggagcc ggccgatgag
     5341 ccggatctcg gactcgaggc ggtcgtggga acgcgcgtcc acctcgtcca ggcggctcgc
     5401 gaggcggcgc agcccgacct gttgctcttc agtgaaatcc ttcacgaggg tccagcggtc
     5461 ctcgaggaga gcgcgctcgt cctcgggcct gttccggaac gacgattcga gcgcgacgag
     5521 ccgtgcgcgt tccttcgcgc tcgcacgctg ccagtcgagg aagcgtcccg tgaggccgtc
     5581 gggcgtgtcc ggcgggcgcg acgggaaccc gccgggcggc accggcacgg gcgcgcgggg
     5641 gccgaccggc tccggaaaga aggcgtcgag gcgcgcgagc gtctcgaacg tgttcaggaa
     5701 gtcgaactgg tgcagggccg ctgcctgcgc cttctcttcg ttcgtgagag gcgcgtagtc
     5761 gaggctgatc ggggtcgagc ccaccgcgac gatgccgggc gggcgctcgg gcggcgccgg
     5821 acggaccttc ggggaggcgg ggggcccggg ccgcgctgga tggtggttaa aatgatcata
     5881 gaa
//
LOCUS       DAOMFY010000248         5872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4608_length_5872_cov_6.307203, whole genome shotgun sequence.
ACCESSION   DAOMFY010000248 DAOMFY010000000
VERSION     DAOMFY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4608_length_5872_cov_6.307203"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PLB01_15860"
     CDS             <1..1053
                     /locus_tag="PLB01_15860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQQ78830.1"
                     /translation="ALVWNVAGRHEMVIEDLLRMKVTATPKLPGDLVSGVTWSRDGKA
                     LAMTASGATAPADIFLLEEISSSSSSSPSSRFTRITESPHPGVDLSSLVSPELLKFSA
                     HDGEPLSGWLYRPRGAKGPGPVVLSFHGGPEGQEVPSFRADYQALVAHGVAVFAPNVR
                     GSSGFGKRFVNLDNGALRVNAVKDIVSCVDAVVKAGVADPKRIGIMGGSYGGYMTMAG
                     LVDYPDMFAAGADLFGVVNFKTFFKHTEAWMAAISKVEYGDPATEGDMLDRLSPLTRI
                     DRVKAPTLVLHGANDTNVPVIEAEQVVDSLKKRGIPVEYVLFPDEGHGFQKLPNRIKA
                     NVTLVKWFVKWLGAGR"
     gene            1067..3199
                     /locus_tag="PLB01_15865"
     CDS             1067..3199
                     /locus_tag="PLB01_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ78831.1"
                     /translation="MTIQPGRRLGPYEVLAPLGAGGMGEVYRARDTRLGREVALKVVT
                     AKHGEDPGALRRFAREAKIAARLTHPNVCTLFDADTDGEVAYFVMELLEGETLASRIA
                     RGPVPKEEALRIAADVARGLARAHELGFVHRDLKPQNIFLTPTGAKILDFGLARSGPA
                     EAAEDQETASLLTEAGRILGTVGYMAPEQVRGEPPAAAADLWALGCVLYECLSGRRTF
                     SGRSAQEAFAATLTSPPDWSALPAGVSSATRTVLQRLLDKDPAGRPSDAAAVERELSG
                     GSAPTGPSIAPAPPRRRKALVTFAVLVVLLAAAAAVWFVREGRAPIDSLAVLPFSSTG
                     SDADLADVCASLSEGVLARISQAPNLRVMASGAVERFSGPHVDPLAAARELGVRAVLT
                     GKAVGRAGRVVVSTELVDARDGRRLWGERYERPVAEIASLPAEISAALAARLRLRLSG
                     SDRARLARKETENGPAYEYWLSGRQRVSRRTESDLGGALADFQKAIDADPQFARAWAG
                     VAQAWDILGYTGRRPTPEAYERSKAAARKAIELDPDLADAHAVLAHATMLTGDDKGAE
                     PEFKKALELDANSVTALHWYSHLLVKQKRWDESLALSKRLLELDPMGYWNVHLGEHYR
                     AKGERALALEQFRRAVELDANLPNAQWELGRFLLEEGRVAEALPHLEIAHRLDPEAPQ
                     ARLSLADAYEKSGRPADAARLRAGAAPR"
     gene            complement(3196..3897)
                     /locus_tag="PLB01_15870"
     CDS             complement(3196..3897)
                     /locus_tag="PLB01_15870"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HQQ78832.1"
                     /translation="MFRAAALLLAVVSAAEDPAALLRRADRVKDAWPEVVITLRVTVT
                     KPGAPPSGGTFKVEAKGRNSRLTFQDPADAGKAVVSKGDDSWLILPGTKNPIRIPKSQ
                     RLSGGFSASEMSKTRFTEDYDAVFERSDVLDGRECAVLRLMARKGRSPTYPVARVWID
                     GKEGLYRKAVFLVASGKTAKETTFDEYRVVRGVLSLSKMTIVDGLRPGTTVVEYLDYE
                     KASLPDAIFEPRAAP"
     gene            complement(3890..5119)
                     /locus_tag="PLB01_15875"
     CDS             complement(3890..5119)
                     /locus_tag="PLB01_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQQ78833.1"
                     /translation="MNRWVLLAARNLMRHRRRTFLTGSIVVVGFVAVTMTAGFVSQTF
                     SALKSATIRGVGGHLRLLDPRAAGKTDDEASTILLDDWPGLAGLLSRDPRVVQAMPKL
                     SFFGLAVKGDKSAAYLGTGTIPALEKQASLAADTVVSGSFMTRNDADEIMLGTGLARA
                     IDAKAGDLVTVMTTTPEGGINAVDATVAAVLAYPIKELDDRLLFMPYGAAARLLKAEG
                     KANSVVVLLKDDRDTELEAVELPKALAAQGRPVALKTWLDTATFYKQVKLLYISIFFF
                     MGLVLAVVVILATANTMTMSVFERTREIGTLLAIGMERSSIRALFLLEGALLGLIGSG
                     IGAALSVLLRALLNASGIMLPPPPGATRGNVLHVDFIPLAYGIGFAVMSITLLVAAWW
                     PARRAARLNPVEALGHV"
     gene            complement(5212..5787)
                     /locus_tag="PLB01_15880"
                     /pseudo
     CDS             complement(5212..5787)
                     /locus_tag="PLB01_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          875 a         2107 c         2031 g          859 t
ORIGIN      
        1 gccctggtgt ggaacgtcgc gggcagacac gagatggtga tcgaagatct tcttcgaatg
       61 aaggtgacgg caacgccgaa gctgccgggc gatctcgtct ctggggtcac gtggtcgcgc
      121 gacggcaagg ctctggccat gaccgcttcg ggcgccacgg ctccggccga catcttctta
      181 ctcgaagaaa tctcctcttc ttcctcctcg tctccttctt cccgtttcac ccgcatcacc
      241 gaatcgcccc acccgggcgt cgacctctcg tcgctcgtgt cccccgagct cctcaagttc
      301 tccgcccacg acggcgagcc gctctcgggc tggctctacc gccctcgcgg cgcgaagggc
      361 ccggggccgg tcgtcctctc gttccacggc ggccccgagg ggcaggaagt cccgtcgttc
      421 cgggccgact accaggcgct cgtcgcgcac ggcgtcgccg tcttcgcgcc gaacgtgcgc
      481 ggctcttccg gcttcggcaa gcgcttcgtg aacctcgaca acggcgcgtt gcgcgtgaac
      541 gccgtcaagg acatcgtgtc gtgcgtggac gccgtcgtga aggcgggcgt cgcggacccc
      601 aagcgcatcg gaatcatggg cggctcgtac ggcggttaca tgacgatggc cggactcgtc
      661 gattatcccg acatgttcgc ggcgggcgcg gacctcttcg gcgtcgtgaa cttcaagacg
      721 ttcttcaagc acaccgaggc gtggatggcg gcgatctcga aggtcgagta cggcgacccc
      781 gccaccgagg gcgacatgct cgaccgcctc tcgccgctca cgcggatcga ccgggtcaag
      841 gcgcccacgc tcgtcctcca cggcgcgaac gacacgaacg tgcccgtcat cgaggccgag
      901 caggtcgtcg acagcctgaa gaagcgcggg attcccgtgg agtacgtcct cttcccagac
      961 gagggccacg gcttccagaa gctcccgaac cggatcaagg cgaacgtcac gctcgtgaag
     1021 tggttcgtga agtggctcgg ggcgggacga tgacggcgca cgcaacgtga cgatccagcc
     1081 cggcaggcgc ctcgggccgt acgaggtcct cgctccgctc ggagccggag gcatgggcga
     1141 ggtgtaccgg gcgcgcgaca cgcgcctcgg ccgcgaggtc gcgctcaagg tcgtcaccgc
     1201 gaagcacggc gaggatccgg gcgccctccg cagattcgcg cgggaagcga agatcgcggc
     1261 gcgcctgacg cacccgaacg tctgcacgct cttcgacgcg gacacggacg gtgaagtcgc
     1321 gtacttcgtc atggagctcc tcgagggcga gacgctcgcc tcgcgcatcg cccgcggacc
     1381 cgttcccaag gaggaggcgc tccgcatcgc ggccgacgtc gcccgcggtc tcgcgcgcgc
     1441 ccacgagctc ggcttcgtcc accgcgacct caagccccag aacattttcc tcacccccac
     1501 cggtgcgaag atcctcgact tcggcctcgc ccggtcgggc ccggccgaag ccgccgaaga
     1561 ccaggagacc gcttcgctcc tcaccgaggc cgggcgcatt ctcggcaccg tcggctacat
     1621 ggcgccggag caggtgcgcg gcgagcctcc cgcggccgcc gccgacctgt gggcgctcgg
     1681 ctgcgttctc tacgagtgcc tctccggccg gcggaccttt tccgggcgca gcgcccagga
     1741 agcgtttgcc gcgacgctca cgagcccgcc ggactggagc gccctgcccg ccggagtctc
     1801 gtcagcgacg cgcaccgtcc tccagcgtct tctcgacaag gatccggcgg ggcgcccctc
     1861 ggacgccgcg gccgtcgagc gcgagctctc gggcggatcc gcgccgactg ggccctcgat
     1921 cgccccggcg ccgccgcggc ggcgcaaggc cctcgtgacc ttcgcggtcc tcgtcgtcct
     1981 cctcgccgcc gcggcggccg tgtggttcgt ccgggaggga cgtgcgccga tcgactcgct
     2041 cgccgtcctg cccttctcgt ccacggggag cgacgccgac ctcgcggacg tgtgcgcgtc
     2101 gctgtcggaa ggggtgctcg cgcggatctc gcaggcgccg aacctccgcg tgatggcctc
     2161 gggcgccgtc gagcgtttct cggggccgca cgtcgacccg ctcgccgccg cccgggagct
     2221 cggcgtgcgt gccgttctca ccgggaaggc agtcggacgc gcggggcggg tcgtcgtctc
     2281 gaccgagctc gtcgacgcac gggacggccg ccgcctctgg ggcgagcgct acgagcggcc
     2341 cgtcgccgag atcgcctcgc ttcccgcgga gatctccgcg gccctcgccg caaggctccg
     2401 gctccgcctg agcggctcgg accgagcgcg gctcgcgcgg aaggagaccg agaacggccc
     2461 cgcctacgag tactggctca gcggccgcca gcgcgtgtcg cgccggacgg agtccgatct
     2521 cggaggcgcc ctcgcggatt tccagaaggc gatcgacgcc gatccgcagt tcgcgcgggc
     2581 gtgggcggga gtcgcccagg cctgggacat cctcggctac accggtcggc gtccgacacc
     2641 cgaggcctac gagaggtcca aggcggccgc ccgcaaggcg atcgagctcg accccgacct
     2701 cgcggacgcc cacgcggtcc tcgcgcacgc gacgatgctg accggagacg acaagggcgc
     2761 cgagccggag ttcaagaagg ctctcgagct cgacgcgaac agcgtcaccg ccctccactg
     2821 gtattcgcac ctcctcgtca aacagaagcg atgggacgag tcgctcgcgc tgagcaagcg
     2881 tctcctcgag ctcgacccca tgggctactg gaacgtccac ctgggcgagc actaccgcgc
     2941 caagggcgag cgggcgctcg ccctcgagca gttccggcgg gcggtcgagc tcgacgcgaa
     3001 ccttccgaac gcacagtggg agctggggcg gttcttgctc gaggaaggcc gcgtcgccga
     3061 agcgcttcca cacctcgaga tcgcccaccg cctcgacccg gaggcccccc aggcgcgcct
     3121 ttctcttgcc gacgcctacg agaaatccgg ccggcccgcg gacgctgcgc ggctgcgcgc
     3181 cggggctgct ccgcgttagg gcgcggccct cggctcgaag atcgcgtccg gcaggctcgc
     3241 tttctcgtag tcgaggtact cgacgaccgt ggtgccgggt cggagcccgt ccacgatcgt
     3301 catcttcgag agagacagga cacccctgac gactcggtac tcgtcgaacg tcgtctcctt
     3361 cgcggtcttc ccggatgcca cgaggaagac ggccttgcgg tagaggccct ccttgccgtc
     3421 gatccacacg cgcgcgaccg gatacgtcgg cgagcggccc tttcgcgcca tgaggcgcag
     3481 gaccgcgcac tcacgcccgt cgaggacgtc cgaacgttcg aacaccgcgt cgtagtcctc
     3541 ggtgaaacgc gtcttcgaca tctcggacgc cgagaagccg cccgagaggc gctggctctt
     3601 cgggatccgg atcgggttct tcgtgcccgg gaggatcagc cacgagtcgt cgcccttcga
     3661 gacgacggcc ttccccgcgt ccgccgggtc ctggaacgtc aggcgtgaat tccgcccctt
     3721 cgcctcgacc ttgaacgtgc ccccggacgg cggagccccg ggcttcgtga cggtcacgcg
     3781 cagcgtgatc acgacctccg gccacgcgtc cttcacacgg tccgcgcggc gcagcagcgc
     3841 cgcgggatct tccgccgcgg atacgacggc caggagcagc gcagcggccc taaacatgcc
     3901 cgagcgcttc cacagggttc aggcgcgccg cgcggcgggc gggccaccag gcggccacga
     3961 ggagcgtgat cgacatgacc gcgaagccga tcccgtaggc gagcgggatg aagtcgacgt
     4021 gcaggacgtt gccgcgcgtc gcgccgggag gcggcggcag catgatcccc gaggcgttca
     4081 gcagggccct cagcagcacc gagagagccg ccccgatccc cgatccgatc agaccgagca
     4141 gcgccccctc gagcaggaac agggcgcgta tcgaagagcg ctccatcccg atggcgagga
     4201 gggtcccgat ctcccgcgtg cgctcgaaga cggacatcgt catcgtgttc gccgtcgcga
     4261 ggatcacgac caccgcgagg acgaggccca tgaagaagaa gatcgagatg tagagcagct
     4321 tcacctgctt gtagaacgtg gccgtgtcca gccacgtctt cagggcgacg ggccggccct
     4381 gcgccgcgag agccttcggc agctccaccg cttcgagctc cgtgtcgcgg tcgtccttga
     4441 gaagcacgac gacggagttc gccttcccct cggccttcag gaggcgggcg gcggcgccgt
     4501 acggcatgaa gaggaggcgg tcgtccagct ccttgatcgg gtacgcgagc acggcggcga
     4561 cggtcgcgtc caccgcgttg atgccgccct cgggcgtcgt cgtcatgacg gtgacgaggt
     4621 cgccggcctt cgcgtcgatc gcgcgggcga gcccggtgcc gagcatgatc tcgtccgcgt
     4681 cgttgcgggt catgaacgac ccggagacga ccgtatccgc cgcgagcgac gcctgctttt
     4741 cgagcgccgg gatcgtgccg gttcccaggt acgcggcgct cttgtcgccc ttcaccgcga
     4801 ggccgaagaa cgacagcttc ggcatggcct gcacgacgcg cgggtcacgg gacaggagcc
     4861 ccgcgaggcc cggccagtcg tcgaggagga tcgtcgaggc ctcgtcgtcc gtcttgccgg
     4921 ccgcgcgcgg gtcgaggaga cggaggtggc cgccgacccc gcggatcgtc gcgctcttga
     4981 gggccgagaa cgtctgggac acgaaccccg ccgtcatcgt gaccgcgacg aagcccacga
     5041 cgacgatcga gcccgtcagg aacgtccggc gacggtggcg catgaggttg cgggcggcga
     5101 ggagaaccca gcggttcacg cgctcacccc cgcggccgcg cggtcgtccg cggtgatccg
     5161 cccgtccagg agacggatgc ggcggtccgc gcgatcgacg atgcgcgggt cgtggctcga
     5221 gaagaggaac gcgacgcccc gctcctcgtg gatccgcgcg aagaggtcga tgagcttccc
     5281 ggcggtctcc gagtcgagcg aggccgtcgg ctcgtccgcg aggacgagcg cgggctcccc
     5341 gacgatcgcg cgggcgatcg cgacccgctg gcgctgcccg cccgacagtt cgtgggggaa
     5401 gcggctggcg aaccccgaca ggcgcacccc gtccagcgca tcggcgacct tggccgggat
     5461 ctcgccggcc ggcgtcttcg ccatcttgag cggaaacgcc acgttggcct gcacggtcat
     5521 gtgggggaac agcgcgtagt tctggaaaac cgtgcgcacc ggccgcgcct cgggcgggag
     5581 ggcggcaagg tcctcgccat tgagcaggat ctgcccgccg tcgggcaggt cgaacccggc
     5641 gatcatgcgc agcagggtcg tcttgccgca gccggacggg cccaggagcg tgaagaactc
     5701 tccggccttg accgacaggc tcacatcgtc gacggcggtg aattccgcga agcggcgggt
     5761 gacgtttcgg acttcgagca gcgccatcgt gcggggattt cccgggaggg gtgtgcgact
     5821 ggcccggatt gtatcggcta ttgtcgcacc gcagtaacgg cgcggcgcgg tc
//
LOCUS       DAOMFY010000249         5859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4621_length_5859_cov_6.531185, whole genome shotgun sequence.
ACCESSION   DAOMFY010000249 DAOMFY010000000
VERSION     DAOMFY010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_5859_cov_6.531185"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLB01_15885"
     CDS             <1..194
                     /locus_tag="PLB01_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78834.1"
                     /translation="VGTELHVTNLQSDEWFTCRVVRTAGKHESGRHALGVEILGTDDK
                     GPAPAGAASAKAKKRKPAH"
     gene            complement(188..1381)
                     /locus_tag="PLB01_15890"
     CDS             complement(188..1381)
                     /locus_tag="PLB01_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQQ78835.1"
                     /translation="MNRVTSTALLILLAAPFVARAATFIPGGERVSLVSKVLGEERTL
                     LVAVPDGYAASNLRYPVLYLTDAETQFAHTSATAGFLARTGSMPGVIVVGIVNTDRSR
                     DLTPTRDPQFANSGGADRFLDFLETELVPFVEARYRTAPYRIFAGHSAGGNFAFHAMR
                     ARPGLFQGVIAVSPWLVWDKRKGLAPLTAFFAGGGVRTRALFFTSGDEGEEMRDVLGQ
                     VSAALKSSSPKGLRWSSAQFPGEHHDSVVLQSHYAGFRMIFDGWSIPRDPETEQLVGS
                     LDDVKKRYADLSERFGFTVTPPEASVNRLGYQALVRKNLPAALGFFRFNASTYPESPN
                     VHDSLGDALEASGQLEEAFASFSRAAELGEKSADANTKIFKANADRVRVKIATRASET
                     PARRQ"
     gene            complement(1450..1683)
                     /locus_tag="PLB01_15895"
     CDS             complement(1450..1683)
                     /locus_tag="PLB01_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78836.1"
                     /translation="MDNKYLHTFSLVVEGLPEPVEFHMFHELEDVAEVTDSFAKYVAR
                     QEDDFLPIGENEAVRANRVLRITHLRSERQPDA"
     gene            1750..3372
                     /locus_tag="PLB01_15900"
     CDS             1750..3372
                     /locus_tag="PLB01_15900"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HQQ78837.1"
                     /translation="MTAPPTRLSVPLWIAAAAAALCLLAAAGRDFWSPDEPDFAEHVR
                     EMLERKSLLLPYENGKPYSEKPILFYWAVAATTPFTGGDVSPLGTRVPSALAAAVLVF
                     GAAALAGRRGGEKEALIAGAATAVAPIVFWQGQYVQVDALFSGLLMLSLLAVFFLEGE
                     ERKASARRWRWVLRVTLAAAVLTKGPLAVVLVGLVVLARCALRRSWGPLLATHPFQSI
                     FIFFLLVLPWYFFAARAGGPAYAYDLIVNQNWNRFFQAFDHIQPWWFYFESMWSDFFP
                     WTALALAAPLVLAPRGLFRERPELGFSATVAAVCFVFLSTSQAKQGKYLLVAYPFAAV
                     LVAALVAVADRPRDPRDAARGYVRGYVGLVAGILLAAAIALGPVVARRAPEFSGLATL
                     VAIPLGVGAIGTFFVLFRRREAAPAVLALAAAVAAEEITAGAVVLPALDVLKTGRPFY
                     ERIRPRVSAGQPLAYFGGTYRCYPILVLRRKTEHFKTDRDLADWLERTPGALVLADES
                     ERQYWKDERLSRLVVLDRQRVGGDVAQLLGLP"
     gene            3369..4070
                     /locus_tag="PLB01_15905"
     CDS             3369..4070
                     /locus_tag="PLB01_15905"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HQQ78838.1"
                     /translation="MTTLADSVTFPAAGVRHPGRHEVLEATFARGEERIDAVVKKVPA
                     GGGRDRAVRSWETAHALVARGIDTPEPLAVGRVGGEGWYVARRLEGAEQIRRWFLARD
                     GAGAPPPSLPYGFEEIVTALARLARRLHDSGVFFRDFTDGNVLVTRGEAGPRLWLVDL
                     DRARVGDAPLPFLGRCRDLARPGLNRPEDLKLLLSRYFEPGRTPAAAAFWVAALRRRI
                     VLWDDFKARARPWRK"
     gene            4129..4815
                     /locus_tag="PLB01_15910"
     CDS             4129..4815
                     /locus_tag="PLB01_15910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ78839.1"
                     /translation="MTAYAMAKVLSFPPRADERASATDGLRREEEPRARAERLAAEAD
                     VIARVLAGDVDAFEYFVKTYQKRITRFVFTLLRDPSDADCVAQDVFVKAFRALPEFKG
                     ESAFETWLTRIAINTVRDRIRRRRPVVSFSDLRDLDDDDGPEIPAALDPADGTSPERD
                     LLSGDIRRRIAVALVSLSPRQRSIFVMKHYEEKSIAEIGDATGLDEGTIKSHLFRAAR
                     KLRVCLEDLR"
     gene            4812..5321
                     /locus_tag="PLB01_15915"
     CDS             4812..5321
                     /locus_tag="PLB01_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78840.1"
                     /translation="MSATPHLSADEIVARVFPEEGGPAAVPAHLASCTACQERVARLR
                     EAWLLDRGAISGYVDGLPESFWSRQASRTMEEIRSSVAPRARVSSVRPRLVRHPFLAF
                     GSLAAALVLVATATVSHFASRPPAVQSAVAASPTPSSVDSSDEELLRAIDRVLDDSSL
                     TRLAAEETL"
     gene            5324..5836
                     /locus_tag="PLB01_15920"
     CDS             5324..5836
                     /locus_tag="PLB01_15920"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HQQ78841.1"
                     /translation="MRLALAAALGAALALPALSQMPETPPGKWWKRPRVVQMLKLTPE
                     QQEKLEEIFARNRKSFVDLKADVEKNQIDVEELVAKKDADPKKVSSAVDALEQSRMRL
                     RKSVTMMFLEQKAVLTSQQWSVLLERRDEWRRERGEERRRGEMERRGDMSPDGAPAPP
                     RSPAGSQPPQ"
BASE COUNT          807 a         2081 c         2047 g          924 t
ORIGIN      
        1 cggtgggtac cgagttgcac gtcacgaatc tccagtcgga cgagtggttc acgtgccgcg
       61 tcgttcggac cgccggaaag cacgagtccg gccgccacgc gctcggcgtc gagatcctgg
      121 gcaccgacga caaggggccc gcgccggcgg gcgccgcctc cgccaaggcc aagaagcgga
      181 agcccgctca ctgacgccgg gcgggcgtct cggaggctcg cgtcgcgatc ttcacgcgca
      241 cgcgatccgc gtttgccttg aagatcttcg tattcgcgtc ggcgctcttt tcgccgagct
      301 cggcggccct cgagaagctc gcgaacgcct cctcgagctg gccgctcgcc tccagcgcat
      361 ccccgaggct gtcgtggacg ttcggggact cgggataggt gctcgcgttg aagcggaaga
      421 agccgagcgc cgccgggagg ttcttccgca cgagcgcctg gtagccgagg cggttcaccg
      481 aggcctccgg cggcgtcacc gtgaagccga aacgctcgct caggtccgcg tatcgcttct
      541 tcacgtcgtc cagcgaaccc accagctgct cggtctccgg gtctctcgga atcgaccagc
      601 cgtcgaagat catccggaat ccggcgtagt ggctctggag gacgaccgag tcgtggtgtt
      661 cgccggggaa ttgcgcgctc gaccagcgga ggcccttcgg cgaggaggac ttcagggcgg
      721 cggagacctg tccgaggacg tcgcgcatct cctcgccttc gtcgccggag gtgaagaaga
      781 gcgcacgggt ccgtacgccg cccccggcga agaacgcggt gagcggcgcg aggccctttc
      841 gcttgtccca cacgagccac gggctcacgg cgatgacccc ctggaacagg cccggccggg
      901 cccgcatcgc gtggaaggca aagttcccgc cggccgagtg gcccgcgaag atccggtagg
      961 gtgcggtgcg gtagcgcgcc tcgacgaacg gaacgagctc cgtctcgagg aagtccagga
     1021 accggtctgc gcccccgctg ttcgcgaact gcgggtctcg ggtcggcgtg agatcccggg
     1081 agcggtcggt gttcacgatg cccacgacga tgacgccggg catggagccg gtccgggcca
     1141 gaaagccggc agtggcgctc gtgtgggcga actgcgtctc ggcgtccgtg aggtagagga
     1201 ccgggtagcg gagattcgac gcggcgtacc cgtccgggac ggcgacgagg agcgtccgct
     1261 cctctcccag caccttcgac acgagggaaa cgcgctcgcc gcccgggatg aacgtcgcgg
     1321 cacgggcgac gaaaggcgct gcgagcagga tgagaagggc ggtgctcgtg acgcgattca
     1381 ttcgtttcct ccctgcagtc ccgtggcgac accgggttta cgggcgggag gcgtggaagt
     1441 tgcgtccgct cacgcgtcgg gctggcgctc gctccggagg tgcgtgatcc tgaggacccg
     1501 gttcgcgcgc accgcctcgt tctcgccgat ggggaggaag tcgtcctcct ggcgtgcgac
     1561 gtatttcgcg aaggaatccg tgacttcggc aacgtcctcg agctcgtgaa acatgtggaa
     1621 ctcgaccggc tcggggaggc cttcgacgac gaggctgaac gtgtgcaggt acttgttgtc
     1681 catgaaaggg cctccgcccc ccattgtcgc ccggagcgcg aaacgccgct cgtcgtatcg
     1741 ttgggccgcg tgaccgcccc tccgacccgc ctctccgtac ccctgtggat cgcggccgcc
     1801 gcggccgcgc tctgcctcct cgcggcggca gggcgcgact tctggtcgcc ggacgagccc
     1861 gacttcgccg agcacgtgcg cgagatgctc gagcgaaaga gcctcctcct cccgtacgag
     1921 aacggaaaac cgtactccga gaagccgatc ctcttctact gggcggtcgc cgcgacgacg
     1981 ccgttcacgg gcggcgacgt gtcgccgctc ggcacgcgcg tcccgtcggc cctcgcggcg
     2041 gcggtcctcg tcttcggcgc cgcggcgctc gcggggcggc gcgggggaga gaaggaagcc
     2101 ctgatcgcgg gcgcggcgac ggcggtcgcg ccgatcgtct tctggcaggg gcagtacgtc
     2161 caggtggacg cgctcttttc cgggttgctg atgctcagtt tgctggctgt ttttttctta
     2221 gaaggtgaag agcggaaggc ttcggcgcgc cgctggcggt gggtcctgcg cgtgacgctc
     2281 gcggccgcgg tcctgaccaa agggcccctc gccgtggtgc tcgtagggct ggtggttctc
     2341 gcccgttgcg ccctgcgccg ctcgtggggg ccgctgctgg ccacgcaccc cttccaaagc
     2401 atcttcatct tctttctcct cgtcctcccc tggtacttct tcgccgcccg agcgggcgga
     2461 cccgcgtacg cgtacgacct gatcgtcaac cagaactgga accggttctt ccaggcgttc
     2521 gaccacatcc agccgtggtg gttctacttc gagagcatgt ggagcgattt cttcccgtgg
     2581 acggctctcg cgctcgccgc gccgctcgtc ctcgcgccgc gcgggctgtt tcgcgagcgg
     2641 ccggagctcg gcttctccgc caccgtcgcc gcagtctgct tcgtcttcct gtcgacgtcg
     2701 caggccaagc agggcaagta cctgctcgtc gcgtacccgt ttgcggcggt tctcgtcgcg
     2761 gcgctcgtcg cggtggccga ccgcccgcgc gacccgcggg acgcggcgcg cggatacgtg
     2821 cgggggtacg tcggcctcgt ggcggggatc ctcctcgccg cggcgatcgc gctggggccg
     2881 gtcgtggcgc gccgggcgcc ggagttctcg ggcctcgcga ccctcgtcgc gattccgctc
     2941 ggcgtcggag cgatcgggac cttcttcgtc ctcttccgcc gccgcgaggc cgcgccggcc
     3001 gtccttgcgc tcgccgcggc ggtcgcggcg gaagaaatca ccgccggcgc cgtcgtgctc
     3061 ccggccctcg acgtcctgaa gaccggccgg ccgttctacg agcgcatccg gccgcgagtc
     3121 tccgcgggcc agccgctcgc gtacttcggc ggcacgtacc ggtgctaccc gatcctggtc
     3181 ctgcgccgca agacggagca cttcaagacc gaccgcgacc tcgccgactg gctcgagcgg
     3241 actcccggcg cactcgtcct ggcggacgag agcgagcggc agtactggaa ggacgagcgg
     3301 ctctcgcgcc tcgtcgtcct cgaccggcag cgggttggcg gagacgtcgc gcagctcctc
     3361 gggctcccat gacgacgctc gccgactccg tcacgtttcc ggcggccgga gtgcgccacc
     3421 ccggacggca cgaggttctc gaggcaacgt tcgcgcgcgg tgaggagcgc atcgacgccg
     3481 tcgtgaagaa ggtcccggcg ggcggcggcc gcgaccgcgc cgttcgatcg tgggagaccg
     3541 cgcacgcgct cgtcgcgcgc ggaatcgaca cgcccgagcc gctcgccgtg ggccgcgtcg
     3601 gcggcgaggg ctggtacgtc gcgcgccggc tagagggcgc cgaacagatt cgccggtggt
     3661 ttctcgcgcg cgacggtgcc ggcgcgcctc cgccatcgct tccgtacggc ttcgaggaga
     3721 tcgtgacggc gctcgcgcgc ctcgcgcggc gcctccacga ttcgggagtc ttcttccggg
     3781 acttcacgga cggcaacgtc ctcgtgacga ggggagaggc cgggccccgc ctctggctcg
     3841 tcgacctgga ccgagcccgc gtcggggacg cgcccctgcc gttcttaggg cgctgccgag
     3901 acctcgcgcg tccgggcctg aaccggccgg aggacctcaa gctgcttctt tccaggtact
     3961 tcgagccggg gcgaacaccc gcggcggccg ctttctgggt tgcggccctg cgccggcgga
     4021 tcgtgctctg ggacgacttc aaggcccggg cccgtccctg gcggaagtga aggggcggca
     4081 agggcttatc cttgtaaacc tgcacgggta cggcggcgtc caactcccgt gacggcatac
     4141 gcgatggcga aggtcctcag cttcccaccc cgggcggacg aacgcgcgag cgcgaccgac
     4201 gggctccgcc gcgaggaaga accgcgtgcg cgcgccgagc gcctggcagc cgaggcggac
     4261 gtcatcgccc gtgtcctggc aggggacgtc gacgcgttcg agtatttcgt gaagacgtac
     4321 cagaagcgga tcacgcgctt cgtcttcacg ctcctgcgcg acccgtccga cgccgattgc
     4381 gtcgcgcagg acgtgttcgt caaggcgttc cgcgcgctcc ccgagttcaa gggcgagtcc
     4441 gcgttcgaga cctggctcac ccgcatcgcg atcaacaccg tgcgcgaccg catccgccgc
     4501 cgccgccccg tcgtgtcgtt ctccgacctg cgcgacctcg acgacgacga cggcccggag
     4561 atccctgccg cgctcgaccc ggcggacggc acgtcgccgg agcgcgacct cctgtcggga
     4621 gacatccgcc gccgcatcgc ggtcgcgctc gtctccctgt ccccgcggca gcgatcgatc
     4681 ttcgtcatga agcactacga ggaaaaatcc atcgccgaga tcggcgacgc gaccggactc
     4741 gacgaaggga caatcaagtc acacctcttc cgcgcggccc gcaagctgcg cgtctgcctg
     4801 gaggacctgc gatgagcgcc acgccccacc tgagcgccga cgagatcgtc gcgcgcgtgt
     4861 tccccgagga aggcggcccg gccgccgtcc cggcgcacct cgcgagctgc accgcgtgcc
     4921 aggagcgggt cgcccgcctg cgcgaggcct ggctcctgga ccgcggggcc atctcgggct
     4981 acgtggacgg cctgcccgag tcgttctgga gcaggcaggc ttcgcgcacc atggaggaga
     5041 tccgctcgtc cgtcgcgccg agggcccgcg tttcgagcgt ccgcccgcgg ctcgtccggc
     5101 acccgttcct cgcgttcggg agcctcgcgg ccgcgctcgt cctcgtggcg accgcgacgg
     5161 tttcccactt cgcgtcccgg ccgcctgccg tgcagagcgc cgtggccgcc agcccgacgc
     5221 cttcgtccgt cgacagctcc gacgaggagc ttctccgcgc gatcgaccgc gttctcgacg
     5281 actcgtcgct cacgcgcctc gcggccgagg agacgctgtg aggatgcgcc tcgccctggc
     5341 cgcggcgctc ggcgccgcac tcgcgcttcc ggcgctttca cagatgcccg agacgcctcc
     5401 gggcaagtgg tggaagaggc cgcgcgtcgt ccagatgctc aagctcacac ccgagcagca
     5461 ggaaaagctc gaggagattt tcgcccgcaa ccggaagtcg ttcgtggacc tcaaggccga
     5521 cgtcgagaag aaccagatcg acgtcgagga gctcgtcgcg aagaaggacg cggatccgaa
     5581 gaaggtctct tcggcggtcg acgcgctcga gcagtcacgg atgaggctcc gaaagtccgt
     5641 gacgatgatg ttcctcgagc agaaggccgt cctcacgtcg cagcagtggt cggtgctcct
     5701 cgagcgccgc gacgagtggc gccgcgagcg aggcgaggag aggcggcgcg gtgagatgga
     5761 gcgccgcggc gacatgtcac cggacggggc gcccgcccct ccgcgctccc cggccggttc
     5821 ccagccgccg caatagcgct cagggcgccg gcgttccga
//
LOCUS       DAOMFY010000250         5841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4637_length_5841_cov_10.912720, whole genome shotgun sequence.
ACCESSION   DAOMFY010000250 DAOMFY010000000
VERSION     DAOMFY010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4637_length_5841_cov_10.912720"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLB01_15925"
     CDS             <1..185
                     /locus_tag="PLB01_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257056.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQQ78842.1"
                     /translation="ERLPAWGRARGVDVRVAPKALTGDNAVMIGFAGLRRLAGGRLGE
                     GLAAVARSRWPLETLD"
     gene            269..2071
                     /gene="lepA"
                     /locus_tag="PLB01_15930"
     CDS             269..2071
                     /gene="lepA"
                     /locus_tag="PLB01_15930"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQQ78843.1"
                     /translation="MTDPSRIRNFSIVAHIDHGKTTLSDRILEITGALTAREMTEQVL
                     DDNPIEKERGITIKARSVTLRYRAEDGRDYVLNLIDTPGHVDFTYEVSRSLAACEGAV
                     LVVDATQGVEAQTLANAYLAIHNDLTIVPCINKVDLPSAVPEMAREQIEEVIGIPARH
                     AVLTSGKVGTGVRDLLEQIVHDIPAPKGDAGAPLKALLFDSWFDVYRGVTCLVRVHEG
                     TIRPGMKIRMMGTGLGGEVQEVGIFNPKQQVVSELSVGEVGYVIAGIKDLHAAKIGDT
                     VTEAERPTAEALPGFQDVKPMVFASIFPTETEAYEDLRDAMEKLQLNDASFTFEPESS
                     TALGFGFRCGYLGLLHMEIVQERLEREWNLSLITTAPSVPYRITTTRGEELEISNPVK
                     LPEPQLIEEMREPYILATIITKDEFLGGILALCQERRGTQVSLEYAGTQRAVLKYELP
                     LNEVILDFYDKLKSLSRGYASFDYQLIDYRPGDLVKLDILINGEPIDALAFIVHRDKA
                     QAKGRSLVEKMKEMIPQQMFEVVIQAAIGSKILARSTVRAMRKNVLAKCYGGDITRKK
                     KLLEKQKEGKRRMKKVGRVDLPQEAFLAVLKVDS"
     gene            2068..2748
                     /gene="lepB"
                     /locus_tag="PLB01_15935"
     CDS             2068..2748
                     /gene="lepB"
                     /locus_tag="PLB01_15935"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQQ78844.1"
                     /translation="MSRPGRFAETAEAVLVAAALALFVKAFVFQIFHVPTASMEDTVL
                     AGDRIVVDKFAYAPHAGPWARFLPYRSLTRGDVVVFRDPRDASRDLLKRAVATAGDRV
                     EQRAKALRVNGVSRAEPYAVHRDGSTYGADAPPALAKRDTFGPAEVPEGRFFAMGDNR
                     DDSQDSRTWGPAPAANLEGRAVCVLWSWAPPDRSFSGRGAALRRTLDTGLHFFARTRW
                     NRTFRAVR"
     gene            2794..3153
                     /locus_tag="PLB01_15940"
     CDS             2794..3153
                     /locus_tag="PLB01_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78845.1"
                     /translation="MRRNGNRGEGAFGLIVGLAILFVAAVSLVRIVPLHIHGNEVLDA
                     MNEAANFGGLKPLEKLRMDVYLKAQDAKVPLQMQDIHVDKNGSYIVISARYKQTADIF
                     GYKYVYTFDKKVEKPVF"
     gene            3161..3571
                     /locus_tag="PLB01_15945"
     CDS             3161..3571
                     /locus_tag="PLB01_15945"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQQ78846.1"
                     /translation="MAKPRDDESPTLTEVRLDVWLDVACLFPTRSQASAACAGGKVDL
                     NGHAAAAHKLVRSGDRLSISFARGRRTFVVKGLAERHVKKSDARALFEETTPPPSAEV
                     LEARRLERMFAPKDAGGARPTKKERRQRERTRGW"
     gene            3565..4551
                     /locus_tag="PLB01_15950"
     CDS             3565..4551
                     /locus_tag="PLB01_15950"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQQ78847.1"
                     /translation="MVRRLARPRLYEILLLADVVLVAVLLRTRARMNVDLVSALLDMR
                     QFVAITGSVLLAGIVLRALLAARRGRKRGAARLRALLRPGSLPDLARLLAVLALTSWA
                     YSWLKVSLPLLRPDVLFDPGLFRIETALHMGVNPGRFLQGLFPYPSLWRALDAYYGAF
                     LWTVMAGLAWFGAALSVRDRARFAAGFTFLWVFGAWFYFAVPSLGPCYVLKDDYTEVR
                     AAMPAQSATMDLLFAHYGRIRSLHRHPEGFDISPYLGVAAMPSLHVAAQAFLMFFARR
                     RSKALFVLFAALTAVTLFTSVVSGWHYAIDGYAGLLLAWACEAAGRRSVPSA"
     gene            4562..5758
                     /locus_tag="PLB01_15955"
     CDS             4562..5758
                     /locus_tag="PLB01_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HQQ78848.1"
                     /translation="MRTILFTGKGGTGKTTVAAATGLTLAAQGLKTLVMSVDAAHSLA
                     DSFDLPGALTDKHRGEAVPVAKNLWIQEVDVTEEIGRHWKDISGYITALLAVTGVEEV
                     LAEELAIFPGMEEVSALLYVNRYAKEKAFDVLILDCAPTGESLRFVSLPPTLDWYMTK
                     LFRIQRSILKVARPVAKKVANVPLPPDRYFDNVQSLAEKLDGVDALLKDPMTTTVRLV
                     TNAEKIVIKETQRAFMYFGLYGFTVDAVIVNRLLPKGMHDPFFDKWRKTQALFLEEAH
                     RYFDPVPLFTLPLRDDQVLGPAALLSLGTELWAGREAAASFRSDAPYRYAKQNGRYVL
                     TLDLPFVAKEDVDLAVASGDLIVRIGNVRHHVPIPRTLAGFTPSGAKVEDGKLTVRFA
                     RATEAS"
BASE COUNT          914 a         2081 c         1947 g          899 t
ORIGIN      
        1 gggagcgcct cccggcgtgg ggaagggcgc gcggcgtcga cgtgcgcgtc gcgccgaagg
       61 cgctcacggg ggacaatgcc gtcatgatcg gcttcgcggg gctgcggcga ctggccgggg
      121 gccgcctcgg cgagggcctc gcggccgtcg cgcgctcgcg ctggccgctc gagacgctcg
      181 actgacgcac ggcgcgccgg gggcagaaaa gaacgcgctc cgctagaatc cgcgctccgc
      241 cgcgcctttt tcgcgccgcg ccccccgcat gaccgatccc tcgcgcatcc gcaacttctc
      301 gatcgtcgcc cacatcgacc acgggaagac gaccctctcc gaccggatcc tcgagatcac
      361 gggcgcgctg acggcgcgcg agatgacgga gcaggtcctc gacgacaacc cgatcgagaa
      421 ggagcgcggg atcacgatca aggcgcggtc cgtgacgctc aggtaccggg ccgaggacgg
      481 acgggactat gtcctgaacc tcatcgacac gcccggccac gtggacttca cgtacgaggt
      541 ctcgcgctcg ctcgcggcgt gcgagggagc cgtcctcgtc gtggacgcga cgcagggcgt
      601 cgaggcccag acgctcgcga acgcctacct cgcgatccac aacgacctca cgatcgtccc
      661 gtgcatcaac aaggtcgacc tcccgtcggc cgtgcccgag atggcgcgcg agcagatcga
      721 ggaggtcatc gggatcccgg cgcgccacgc ggtgctcacg tccggcaagg tcgggacggg
      781 cgtgcgcgac ctcctcgagc agatcgtgca cgacatcccg gcgccgaaag gcgacgccgg
      841 ggctcccctc aaggccctcc ttttcgattc ctggttcgac gtctaccgcg gcgtcacgtg
      901 cctcgtgcgc gtgcacgagg ggacgatccg gccgggcatg aagatccgga tgatggggac
      961 cggcctcggc ggcgaggtcc aggaagtcgg gatcttcaac ccgaagcagc aggtcgtctc
     1021 cgagctctcg gtgggcgagg tcggatacgt catcgcgggc atcaaggacc tccacgccgc
     1081 caagatcggc gacacggtca ccgaggccga gcgtccgacg gccgaagcgc tgcccggctt
     1141 ccaggacgtc aagccgatgg tcttcgcgtc gatcttcccg acggagacgg aggcgtacga
     1201 ggacctccgc gacgcgatgg agaagctcca gctaaacgac gcctccttca cgttcgagcc
     1261 cgagtcctcg accgcgctcg gcttcggctt ccggtgcggg tacctcggcc tcctccacat
     1321 ggagatcgtc caggagaggc tcgaacgcga gtggaacctc tctctcatca ccacggcgcc
     1381 gtccgtcccg taccggatca cgacgacccg cggcgaggag ctcgagatct cgaaccccgt
     1441 gaagcttccc gagccgcagc tcatcgagga gatgcgcgag ccgtacatcc tcgcgacgat
     1501 catcacgaag gacgagttcc tcggcggaat cctcgccctc tgccaggagc gacgggggac
     1561 gcaggtgtcg ctcgagtacg cggggacgca gcgcgccgtg ctcaagtacg agctcccgct
     1621 caacgaggtc attctcgact tctacgacaa gctcaagtcg ctctcgcggg gttacgcgtc
     1681 gttcgactac cagctcatcg actaccggcc cggcgacctc gtgaagctcg acatcctcat
     1741 caacggcgag ccgatcgacg cgcttgcctt catcgtccac cgcgacaagg cgcaggccaa
     1801 gggccgctcg ctggtcgaga agatgaagga aatgatcccg cagcagatgt tcgaggtcgt
     1861 gatccaggcc gcgatcggct cgaagatcct cgcgcgctcg acggtccggg cgatgcgcaa
     1921 gaacgtcctc gcaaagtgct acggcggcga catcacgcgc aagaagaagc tcctcgagaa
     1981 gcagaaggaa ggcaagcgcc ggatgaagaa ggtcggccgc gtcgacctcc cccaggaggc
     2041 cttcctcgcc gtcctcaagg tcgactcgtg agccgtcccg ggcgcttcgc ggagacggcg
     2101 gaggccgtcc tcgtcgcggc cgcgctcgcg ctcttcgtca aggccttcgt cttccagatc
     2161 ttccacgtcc cgacggcgtc catggaagac acggtcctcg cgggggaccg catcgtcgtg
     2221 gacaagttcg cctacgcgcc gcacgcgggg ccgtgggcgc ggttcctgcc ctaccggagc
     2281 ctgacgcggg gcgacgtcgt cgtgttccgc gacccgcgcg acgcgtcgcg cgacctcctg
     2341 aagcgcgccg tcgcgaccgc cggcgatcgg gtggagcagc gcgcgaaggc gctgcgcgtg
     2401 aacggtgtct cccgcgcgga gccctacgcc gtccaccgcg acggttccac gtacggcgcc
     2461 gacgcgccgc cggcgctcgc gaagcgggac acgttcgggc ccgccgaggt cccggagggc
     2521 cggttcttcg ccatgggcga caaccgggac gactcgcagg actcgcgcac atgggggccc
     2581 gccccggccg cgaacctcga gggccgtgcc gtctgcgtcc tctggtcgtg ggcgcccccc
     2641 gacaggtcgt tttcggggcg gggcgcggcg ttacgccgga cgctcgacac cgggttacac
     2701 tttttcgcca ggacgcgctg gaaccggacg ttccgggcgg tccgctgagg aacattcggt
     2761 ccggacggcc gcgtcgaaga gaggagttcg cacatgcgtc gcaacgggaa tcgtggggag
     2821 ggcgccttcg ggctgatcgt cggtcttgcg atcctgttcg tggccgcggt ctcgctcgtg
     2881 cggatcgttc cgctgcacat acacggcaac gaggtcctcg acgccatgaa cgaggcggcg
     2941 aacttcggcg ggctcaagcc gctcgaaaaa ctccggatgg acgtgtacct caaggcgcag
     3001 gacgcgaagg tcccgctcca gatgcaggac atccacgtcg acaagaacgg aagctacatc
     3061 gtgatctcgg cgcggtacaa gcagaccgcc gacatcttcg gctacaagta cgtctacacg
     3121 ttcgacaaga aagtcgaaaa gcccgtcttc tagccgcgcg atggcgaagc cgcgcgacga
     3181 cgagagcccg acgctcaccg aggtccggct ggacgtgtgg ctggacgtcg cgtgcctctt
     3241 cccgacgcgc tcgcaggcgt cggcggcctg cgccggcggc aaggtggacc tgaacggcca
     3301 tgcggccgcg gcgcacaagc tcgtccggtc cggggaccgc ctgtcgatct cgttcgcgcg
     3361 cggcaggcga acgttcgtcg tgaaggggct cgccgagcgg cacgtcaaga agtccgacgc
     3421 gcgcgctctc ttcgaggaga cgacgccgcc cccttccgcg gaagtcctcg aggcgcggag
     3481 gctcgagcgg atgttcgcgc cgaaggacgc gggcggcgcc cgccccacca agaaggaacg
     3541 gcggcagcgc gagaggacgc gcgggtggtg aggcgcctcg cgcgcccgag actctacgag
     3601 atcctcctcc tcgccgacgt cgttctcgtc gcggttctcc tccggacacg cgcccggatg
     3661 aacgtcgacc tcgtgagcgc gctcctcgac atgcgccagt tcgtcgcgat cacgggatcc
     3721 gtcctcctcg cgggcatcgt cctgcgcgcg ctcctcgcgg cgcgccgcgg gaggaagcgc
     3781 ggagcggccc ggctccgcgc gctcctccgg ccgggatccc ttccggacct cgcgcggctc
     3841 ctcgcggtcc tcgcgctcac gtcctgggcc tacagctggc tcaaggtctc cctcccgctc
     3901 ctccgccccg acgtcctctt cgaccccggc ctgttccgga tcgagacggc gcttcacatg
     3961 ggcgtgaacc ccggacgctt cctccagggc ctcttcccgt atcccagcct gtggcgcgcc
     4021 ctcgacgcct actacggcgc gttcctctgg accgtcatgg cgggcctcgc ctggttcggg
     4081 gcggcgctct ccgtgcgcga ccgcgcgcgt ttcgcggcgg gcttcacgtt cctctgggtc
     4141 ttcggcgcat ggttctactt cgcggtgccg tcgctcgggc cctgctacgt cctcaaggac
     4201 gactacaccg aggtccgcgc cgcgatgccg gcgcagtccg cgacgatgga ccttctcttc
     4261 gcgcactacg gacggatccg ttcgctccac cgtcacccgg aggggttcga catctccccg
     4321 tacctcgggg tggccgcgat gccctcgctg cacgtcgcgg cccaggcgtt cctcatgttc
     4381 ttcgcgcgcc ggcggtcgaa ggctctcttc gtcctcttcg cggccctgac cgcggtgacc
     4441 ctcttcacct ccgtcgtgag cggctggcac tacgcgatcg acgggtacgc gggccttctc
     4501 ctcgcgtggg cctgcgaggc ggccggaagg cgcagcgtgc cgtccgcgta acatcctcgg
     4561 gatgcggacc atcctcttca cgggcaaggg cgggaccggc aagacgacgg tcgcggccgc
     4621 gacgggcctg acgcttgccg cgcagggcct gaagacgctc gtgatgtccg tggacgccgc
     4681 acattctctc gccgattcgt tcgatcttcc cggcgccctc acggacaagc accgcggcga
     4741 ggccgtgccg gtcgcgaaga acctctggat ccaggaagtg gacgtcacgg aggagatcgg
     4801 gcgccactgg aaggacatct cgggatacat cacggcgctg ctcgccgtga cgggcgtcga
     4861 ggaagtcctc gccgaggagc tcgcgatctt ccccggaatg gaggaggtct ccgccctcct
     4921 ctacgtgaac cgctacgcga aggagaaggc gttcgacgtc ctgatcctcg actgcgcgcc
     4981 cacgggcgag tcgctgcgct tcgtctcgct gcctccgacg ctcgactggt acatgacgaa
     5041 gctcttccgg atccagcgca gcatcctgaa ggtcgcccgg cccgtcgcga agaaggtcgc
     5101 gaacgttccg ctcccgccgg accggtactt cgacaacgtg cagtccctcg ccgagaagct
     5161 ggacggcgtg gacgcgctcc tgaaggaccc catgacgacg accgtccggc tcgtcacgaa
     5221 cgccgagaag atcgtgatca aggagacaca gcgagccttc atgtacttcg gcctctacgg
     5281 cttcaccgtg gacgccgtga tcgtgaaccg cctcctcccc aagggaatgc acgacccgtt
     5341 cttcgacaag tggcggaaga cgcaggccct gttcctcgag gaggcgcacc gctacttcga
     5401 ccccgttccg ctgttcacgc tcccccttcg cgacgaccag gtgctcgggc cggcggcgct
     5461 gctctcgctc ggaaccgagc tgtgggcggg ccgcgaagcg gcggcgtcgt tccgctcgga
     5521 tgcgccctac cggtacgcga agcagaacgg acgctacgtc ctcacgctcg atcttccttt
     5581 cgtcgcgaag gaggacgtcg acctcgcggt cgcgtccggc gacctcatcg tccgcatcgg
     5641 caacgtccgg caccacgtcc cgatcccgcg gacgctcgcg ggattcacgc cgtccggcgc
     5701 gaaggtcgaa gacggcaagc tcaccgtgcg cttcgcgcgc gcgacggagg catcctgatg
     5761 gcacgcaaga agaagaccgc cgatcccctg gaggccgact tcgggcccgg cgtcgcggcc
     5821 ggcggcggcg cggaggaggg c
//
LOCUS       DAOMFY010000251         5803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4674_length_5803_cov_6.825505, whole genome shotgun sequence.
ACCESSION   DAOMFY010000251 DAOMFY010000000
VERSION     DAOMFY010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4674_length_5803_cov_6.825505"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /gene="pilQ"
                     /locus_tag="PLB01_15960"
     CDS             <1..1479
                     /gene="pilQ"
                     /locus_tag="PLB01_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HQQ78849.1"
                     /translation="AAPAPAASDAPRLVTATPEIRLIPGEKPAPVATAASRPVSPAAE
                     PAPKRRKTSEDKALIEAAETLLNQQDAAGKPREIANPFEARTLGAGDKQYTGEPITLN
                     LKDADIKDTLQRFSELTQLNIVLDPDVRGTVTVSLQEIPWDQALELILKINQLGYVLE
                     GNVMRIASSNKLLQEEQARLAFSAAQDKNRPLRTVLQKISYSNVNEMAATARKVMSSR
                     GDIFVDARSNTLVIKELPDYLPTVLDLIKNLDVASPQVMIEARIIEANRTFSNEIGVV
                     WGFQGVADAAHGNTTGLVFPNSGNVAGNVSLPSGAPQVLSLSLANVLDTFRLDASIHA
                     AESRGLVKVVSTPKVQTQTGEAASIQSGFQIPVQTTVNNTTSVLYINATLRLDVTPQI
                     TNEGTVIMDVTVQKREPAVGINIAGGQNIPLTTRDAKTRLMVRDGGTAVIGGIFKITS
                     NDGQSMIPGLWKIPIIGNLFRSRTQSENTDELMIFITPRIIR"
     gene            1494..2084
                     /locus_tag="PLB01_15965"
     CDS             1494..2084
                     /locus_tag="PLB01_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78850.1"
                     /translation="MKKTTLPVLAIGALLLALLPACSTNTQGDDGAPVFLLGEFNELL
                     LEKLLVEGTPVQFKTTTLRCRLKVPGSGTVQFLDVQVDSYVVRWTRLDGGTKASASET
                     FGGNIIVPVGGTATLNNYQYMTADALLRSPLDQLYPFNGGIDRETGKTSIRQAGHVIW
                     YGHTLSGQPVVSNEATFDLTFRFSAASGRIEGTLAR"
     gene            2098..4695
                     /locus_tag="PLB01_15970"
     CDS             2098..4695
                     /locus_tag="PLB01_15970"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HQQ78851.1"
                     /translation="MRRIPFPAAAMAVGLVALLTGCAGETPTSPSTNGGGGSGGTGTC
                     TVSIAMLSTSLSPTVGTEVVVRASVTKGGAAVPDGTSVQMTTDLGVFAENGLPTVSKT
                     TVSGAADITLFSQFSGAAHVKAAYDCGSSTLTINYSGASTLGPYVSSYSPTTGICTGG
                     DTVTLLGGLFGTTPGIVYFGGAPASLVSWATNTITVKTPVRTLKNSSIPEQVDVVVSA
                     AGTTTTAPIKFTYFCIDPSQRMSVSSINPIAGSPGGGDAVVILGSHFGLNISTTQVTF
                     CGLPAQITGQSDTQISVSTPAHTLANPALSEACPVVVTRDLGLVSTQNATSPVPFTYR
                     GSGSTGACNADPGFFVTSLRPTSGSPDGGDVVTITGSGFPTNATLLRVDFGGSPGTIV
                     GSPTTTTFQVSTPRRVLATPDVPETVDVVVTDLGSATQRCARVGGAFVYTAKALDPAI
                     YGNSPVTGPNDQSTRVTLFGTNFQFPMQVFMTGGTSCGAQRVEAAQPIQVSPTQIIFN
                     TPVANGGNACLAGQLVDIVIVNPSTGKTAKCTSCFKYYSCPVAGVASPSVGSTTQPTT
                     VVVSGNNFPEPTIANFRLASSGTPLTSLQVTSVSNTSIIVTLPSLQTLLGGSLSCGNT
                     DGFIDLTFPGITCVPNTISVPFAYHSDPPTASTASPNNLSQDGSAYPATGSPATITLL
                     GTNFQAPMTVTLIKDGVPVPNTPINNASVSNPTALTFAAPAVPNNSLNQQNCSSGGPI
                     NGLKYVPTSFGIRVRNTLTGCSVDLPNVLVYYPIDTTCRAALQIVTPSLPNGTAGAAY
                     SAQFIASGGAGAPYSWSAVGLPAALVLNAGTGVISGTPAAAGTSTINVTVVDAAANTS
                     TRIYTLQVN"
     gene            4790..>5803
                     /locus_tag="PLB01_15975"
     CDS             4790..>5803
                     /locus_tag="PLB01_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ78852.1"
                     /translation="MALFGGPTQPVTRLEELKRLLQRDPTSRQFLALAEEFRRHGKYR
                     DAVITLERGLALHTNSVAGHVALGKTYQQLDRLEDAIRAFTNALRIDRENLVAIRQLA
                     EVYLAKGDKIEAIKKLKLYRGLKAGDRDVNDIIARLEQEMTAAVGERAGFRSGARPAV
                     EAPPAFVPGPASSSTLRRAALLRKLETRAPDATAPPFPVESRPADPMGLTYDGASSAR
                     ALERSRPIDVPPPPAPPPPLEDLFVQTTPEPAAPSVPAIEVSPFTAPIPQMRDAAPAL
                     DVPAPPAPFEVSPPEVDLDDVPTAPFQFPVDLYRVGSAPEPAAAPPEAPAPAAAAPEC
                     SGVL"
BASE COUNT          957 a         2181 c         1807 g          858 t
ORIGIN      
        1 gcggctcccg cgcccgccgc ttccgacgcg ccgcgcctcg tgacggcgac gccggagatc
       61 cggctgatcc cgggcgagaa gcccgcgccc gtcgcgaccg ccgcctcccg ccccgtttcc
      121 cccgccgccg agccggcgcc gaaacgccgg aagacgtccg aggacaaggc gctcatcgag
      181 gcggccgaaa cgctcctgaa ccagcaggac gcggcgggga agccgcgcga gatcgcgaac
      241 cccttcgagg cgcgcacgct cggcgccggc gacaagcagt acacgggcga gccgatcacg
      301 ctcaacctca aggacgcgga catcaaggac acgctccaga ggttcagcga gctcacccag
      361 ctcaacatcg tcctcgaccc cgacgtgcgc ggcacggtca cggtctccct gcaggagatt
      421 ccgtgggacc aggcgctcga gctgatcctc aagatcaacc agctcggcta cgtgctcgaa
      481 ggcaacgtca tgcggatcgc gtcgtcgaac aagctcctcc aggaggagca ggcgcgcctc
      541 gcgttctcgg cggcgcagga caagaaccgc ccgctccgca cggtcctcca gaagatctcg
      601 tactccaacg tgaacgagat ggcggccacg gcccgcaagg tcatgtcctc gcgcggcgac
      661 atcttcgtgg acgcccggtc gaacacgctc gtgatcaagg agctgccgga ctacctgccg
      721 acggtcctcg acctcatcaa gaacctcgac gtcgcgagtc cccaggtcat gatcgaggcc
      781 cgcatcatcg aggcgaaccg cacgttctcg aacgagatcg gcgtcgtgtg gggcttccag
      841 ggagtcgcgg acgccgccca cggcaacacg acgggcctcg tcttcccgaa ctcggggaac
      901 gtcgcgggca acgtctcgct cccgagcggc gcgccgcagg tcctgagcct gtcgctcgcg
      961 aacgtcctcg acacgttccg gctggacgcg tcgatccacg cggcggagtc gcgaggcctc
     1021 gtgaaggtcg tgtcgacgcc gaaggtccag acgcagacgg gggaggccgc gagcatccag
     1081 tcgggcttcc agatcccggt ccagacgacc gtcaacaaca cgacgtccgt cctctacatc
     1141 aacgcgacgc tccgcctcga cgtgaccccg cagatcacga acgaaggcac ggtcatcatg
     1201 gacgtgaccg tccagaagcg cgaacccgcg gtcggcatca acatcgccgg cggccagaac
     1261 atcccgctca cgacgcgcga cgccaagacg cgcctcatgg tgcgcgacgg tgggacggcg
     1321 gtcatcggcg gcatcttcaa gatcacgagc aacgacggcc aatcgatgat ccccgggctc
     1381 tggaaaatcc cgatcatcgg gaacctcttc cggagtcgca cccagagcga gaacaccgac
     1441 gaactcatga tcttcatcac tccgcggatc atccgctgac aggagcgcgt gcgatgaaga
     1501 agacgactct ccccgttctg gccatcggag ccctgctcct ggcgctcctt ccggcctgct
     1561 cgacgaacac gcagggcgac gacggcgcac cggtcttcct cctcggggag ttcaacgagc
     1621 tccttctcga gaagctgctc gtcgagggca cccccgtgca gttcaagacg acgacgctgc
     1681 gctgccgcct caaggtgccc ggctcgggta cggtgcagtt cctcgacgtg caggtggaca
     1741 gctacgtcgt gcgctggacc cggctcgacg gcgggacgaa ggcctccgca tcggagacgt
     1801 tcggaggaaa catcatcgtg ccggtgggcg ggacggcgac gctcaacaac taccagtaca
     1861 tgaccgcgga cgcccttctg cggtcccctc tcgaccagct ctatccgttc aacggcggca
     1921 tcgaccgcga gaccgggaag acctcgatcc gtcaggcggg ccacgtcatc tggtacggcc
     1981 atacgctctc gggtcaaccc gtcgtgtcga acgaggccac gttcgacctc acgttccggt
     2041 tcagcgctgc gtccggccga atcgagggaa cccttgctcg gtgacgaggt gaaacccatg
     2101 cgacgaatcc cgtttccggc cgccgcgatg gccgtcggcc tcgtggccct cctcaccggt
     2161 tgcgccggcg agacgcccac gtctccatcc accaacggcg gcggcggcag cggcgggacc
     2221 gggacctgta cggtctcgat cgcgatgctg tcgacgtccc tctcgcccac cgtgggaacc
     2281 gaggtcgtcg tgcgcgcttc ggtgacgaag ggcggcgcgg cggtccccga cggaacctcc
     2341 gtgcagatga cgacggacct cggcgtcttc gccgagaacg gcctgcccac cgtgtccaag
     2401 acgacggttt ccggcgcggc cgacatcacg ctcttctcgc agttctccgg cgccgcgcac
     2461 gtgaaagcgg cctacgactg cggatccagc accctcacga tcaactactc gggcgcgtcg
     2521 acgctgggcc cctatgtgtc ctcgtactcg ccgacgaccg gaatctgcac gggcggcgac
     2581 accgtcacgc tcctcggcgg cctcttcggg accacgccgg gcatcgtcta cttcggcggc
     2641 gctccggcct cgctcgtgag ctgggcgacc aacacgatca ccgtcaagac gccggtgagg
     2701 acgctcaaga actcttcgat tcccgagcag gtggacgtcg tggtttcggc ggccgggacg
     2761 acgaccacgg cgccgatcaa gttcacgtac ttctgcatcg acccgtcgca gcgcatgagc
     2821 gtctcctcga tcaacccgat cgccggttcg ccgggcggcg gcgacgccgt cgtgatcctc
     2881 ggcagccact tcggcctgaa catctcgacg acccaggtga cgttctgcgg cctccccgcg
     2941 cagatcacgg gccagtcgga cacgcagatc tcggtctcga cgccggccca cacgctggcc
     3001 aacccggccc tctcggaggc gtgtccggtc gtcgtgacgc gcgacctcgg gctggtctcc
     3061 acccagaacg cgacgtcgcc cgtgccgttc acgtatcgcg gcagcggctc gacgggcgcc
     3121 tgcaacgcag accccggctt cttcgtgacg agcctcaggc cgacctcggg cagccccgac
     3181 ggcggcgacg tcgtgacgat caccggctcg ggcttcccga cgaacgcgac gctcctccgg
     3241 gtcgatttcg gcggcagccc cggcacgatc gtcgggagtc cgacgacgac gacgttccag
     3301 gtctcgactc cccgccgggt cctcgcgacg ccggacgtgc ccgagaccgt cgacgtcgtc
     3361 gtgacggacc tcggcagcgc gacgcagcgc tgtgcccgcg tcggcggcgc cttcgtctac
     3421 acggccaagg cgctcgaccc cgcgatctac ggcaactcgc cggtcacggg cccgaacgac
     3481 cagtcgacgc gcgtcacgct gttcggcacg aacttccagt tcccgatgca ggtcttcatg
     3541 acgggcggaa cgtcctgcgg cgcccagcgc gtggaagcgg cccagcccat ccaggtgtcc
     3601 ccgacgcaga tcatcttcaa caccccggtt gcgaacggcg ggaacgcctg tctcgcgggc
     3661 cagctcgtcg acatcgtcat cgtgaacccg tcgaccggca agacggccaa gtgcacgtcg
     3721 tgcttcaagt actactcgtg cccggtcgcc ggcgtcgcgt ccccctccgt cgggagcacg
     3781 acgcagccga cgacggtcgt ggtctcgggc aacaacttcc ccgagccgac gatcgcgaac
     3841 ttccgcctcg cctcctcggg cactcctctc acgtccctcc aggtcacgag cgtttccaac
     3901 acctcgatca tcgtcacgct gccgtcgctt cagacgctgc tcggcggctc gctgagctgc
     3961 gggaacacgg acggcttcat cgacctcacg ttcccgggaa tcacgtgcgt cccgaacacg
     4021 atctccgtgc cgttcgcgta ccacagcgac ccgcccacgg cgtcgaccgc ctcgccgaac
     4081 aacctgagcc aggacggctc ggcgtatccg gcgacgggct cgcccgcgac gatcacgctt
     4141 ctcgggacga acttccaggc cccgatgacc gtcacgctca tcaaggacgg cgtcccggtg
     4201 ccgaacacgc cgatcaacaa cgcgtccgtt tcgaacccga cggctctcac gttcgcggcg
     4261 cccgcggtcc cgaacaactc tctcaaccag cagaactgct cgtcgggcgg gccgatcaac
     4321 ggcctcaagt acgtcccgac gtcgttcggc atccgggtga ggaacacgct cacgggttgc
     4381 tcggtcgacc tgccgaacgt cctcgtctac tacccgatcg acacgacgtg ccgtgcggcc
     4441 cttcagatcg tcacgccgtc gctgccgaac ggcacggcgg gagcagccta ctcggcgcag
     4501 ttcatcgcct cgggtggcgc gggcgcgccg tactcgtggt cggccgtcgg cctcccggcg
     4561 gcgctcgtgc tcaatgccgg aacgggcgtc atctcgggca cgcccgccgc ggccggtacc
     4621 tccaccatca acgtcacggt cgtagacgcg gccgccaaca cctcgacccg catctacacg
     4681 ctgcaggtca actgacgccg taaccaggcc tccggggttc gggccgcctt cgggcggccc
     4741 gaactgtctc ggaaccggat gccgtagact cggagacgga tcgaatcaca tggctctctt
     4801 cggcggcccg acccagcccg tcacccgtct cgaggagctg aaaaggctcc tccagcggga
     4861 ccccacgtcg cgccagttcc tggcgctggc cgaggagttc cggcggcacg ggaagtaccg
     4921 cgacgccgtc atcaccctgg agagaggcct cgccctccat acgaactcgg ttgccggcca
     4981 cgtcgcgctg gggaagacgt accagcagct cgaccgcctc gaggacgcga tccgggcgtt
     5041 caccaacgcc cttcggatcg accgcgagaa cctcgtcgcg atccggcagc tcgccgaggt
     5101 ctaccttgcc aagggcgaca agatcgaggc gatcaagaag ctgaagctct atcgcggcct
     5161 caaggccggg gaccgcgacg tgaacgacat catcgcgcgc ctcgagcagg agatgaccgc
     5221 cgccgtcggc gagcgtgccg gattccgcag cggggcccgc cccgcggtcg aggcgccgcc
     5281 ggcgttcgtc ccgggcccgg cgtcgtcgag cacgctacgc cgcgccgcgc tcctcaggaa
     5341 gctcgagacg cgcgctcccg atgcgacggc gccccccttc ccggtggagt cgcggcccgc
     5401 cgacccgatg ggcctcacgt acgacggcgc gtcgagcgca cgcgccctgg aacgctcccg
     5461 gccgatcgac gtgccgccgc cacccgcgcc gccgccgccg ctcgaagacc tcttcgtcca
     5521 gacgacgccc gagccggccg cgcccagcgt cccggccatc gaggtttctc ctttcacggc
     5581 accgattccc cagatgcgcg atgcggctcc ggccctcgac gtgccggctc ctccggcccc
     5641 cttcgaagtc tcaccgccgg aagtcgacct cgacgacgtg ccgaccgctc ccttccagtt
     5701 tccggtggat ctgtaccgcg tcgggtcggc gccggagccc gcggcggctc ctcccgaagc
     5761 cccggcgcct gctgccgctg cgccggaatg ttcaggtgtg ttg
//
LOCUS       DAOMFY010000252         5780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4711_length_5780_cov_6.166987, whole genome shotgun sequence.
ACCESSION   DAOMFY010000252 DAOMFY010000000
VERSION     DAOMFY010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4711_length_5780_cov_6.166987"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLB01_15980"
     CDS             complement(<1..283)
                     /locus_tag="PLB01_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78853.1"
                     /translation="MTRVRRLGTLAAAGLLAAAAGCVSGTDIDGLHKHVSVVEKQVDT
                     LQKQSSSKDEMAKLNDSLAKQSATLLRSNADLGAKFDELTREMQSLAGKL"
     gene            complement(280..834)
                     /gene="pal"
                     /locus_tag="PLB01_15985"
     CDS             complement(280..834)
                     /gene="pal"
                     /locus_tag="PLB01_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQQ78854.1"
                     /translation="MKNLLKSGSLALAAVALVVACSPKPAPEPPPPPPAATPTPTIVT
                     LEPTPAPTPKPTPRSQEDDIKAMSLDRVSSYLKPVFFDYDKADLRSDARDVLAANAAW
                     LKSHPTILFTIEGHCDERGTAQYNLALGDRRANSAKEYLVSLGVDAGRVKTVSYGKER
                     PFATGHDEDSWAKNRRGHFVVTAK"
     gene            complement(914..2293)
                     /locus_tag="PLB01_15990"
     CDS             complement(914..2293)
                     /locus_tag="PLB01_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78855.1"
                     /translation="MNRPTPIRTLAALVLAAAAAGASAQPTPANGKPGTGPVAPPVSS
                     LPSQVVVDILGAKGARIPLAIPVTIASLKPELQKGAVDPFFTTLNDDLSGSRVFLVSD
                     PTLYPKGLRPPRNRSEGDQWKASSAQYLLDTRLAQETDASIVVEAQLYDLGTLQPILA
                     KMYSGPLAASRRIAHTLANDLTRQFTGKPGPYLTKIAFVSDRDEPRVKEIYTMDFDGE
                     TQRRVTNSRTLSLAPDWSPDGKKIVYQSYEKDNPGVFLVGRDGTDKFRVPLSTQLNAS
                     PSFSPDGKTIAFCGSVKGNPEIYTVQTDGSNLKRLTENVAIDSTPRWSPNGRELAFTS
                     NRQGSPQIYMMDLEGANVRRVSLAGSWNDEAAFSPDGGRIAFACRNEGDFQICVMEIG
                     TGRTLQISEGPGAHENPTWSPDGTKVAWEVTRGSSTQIAVANADGSAMRIVTSAGNNY
                     SPAWIKTLE"
     gene            complement(2315..3109)
                     /locus_tag="PLB01_15995"
     CDS             complement(2315..3109)
                     /locus_tag="PLB01_15995"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQQ78856.1"
                     /translation="MTVNTTAVGAPLTVSEILARRARGGRAPAPLSAGLSILAHAAFV
                     AAVILTSRPRHVPAAIPISLPVRVVSPAALGRPEAAPAPVAAVPVPEPAPEPVKSRPV
                     IEKKQVEKIEPSPNAMPSLKPAKAKPTPPKPAAAPGKGAPAVDLPSAPGLPSGTAGGT
                     GAALSFGADVAAFDADFPFSYYVQQLVSLIGANWFRPDAPDGSLCTVSFRIQRSGQIS
                     DVKIESGSGASYYDRAAVRAVYAANPLPPLPNDYRNEQLGVHLRFR"
     gene            complement(3123..3545)
                     /gene="tolR"
                     /locus_tag="PLB01_16000"
     CDS             complement(3123..3545)
                     /gene="tolR"
                     /locus_tag="PLB01_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HQQ78857.1"
                     /translation="MAFKLDDEADSFHDINVTPLVDVMLVLLVIFMVTAPLLHQGVEV
                     QLPKSVAQNLPKTPEDPLILSITKSGNYYLNETPVAKSQLKDRLQLVLSRRRDKSVYL
                     KADRNLPYGSVVEMMDTLNRLGVESLGMVTELKSEDRR"
     gene            complement(3553..4248)
                     /locus_tag="PLB01_16005"
     CDS             complement(3553..4248)
                     /locus_tag="PLB01_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQQ78858.1"
                     /translation="MPESNGSNLVQLFLTAGPTAKAVLVVLAFFSLVSWAIIIGKIWS
                     FSRAGASSRAFLAHFRKSRKFADVLGICDRCEPSPLVGIFRAGYAELDQQVKDGREVA
                     PADTGRLFVKSLASIERALERAAGVETTRLTRGLSFLATTASATPFIGLFGTVMGILI
                     SFSQIASKGSTSIVVVAPGIAEALINTAAGLVAAVPALIAYNNFNARVRAARGEMRDF
                     ALEFMNLAERHFT"
     gene            4460..>5780
                     /locus_tag="PLB01_16010"
     CDS             4460..>5780
                     /locus_tag="PLB01_16010"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HQQ78859.1"
                     /translation="MKGWGLTVMKGAVPERFEVEVLGVLPNTLGRSRILVKVSGLGLE
                     RTGVIAGMSGSPVYLDGKLAGAVSATWAFGKDPVGQVTPIENMLEGGSGGASPDAPRA
                     RATVSSAAAFDALASAHGLPPDERVAALEKIAAPYRPAAGREAASLLAPVSAGLPSAT
                     LSRFAEDLGRLGLPAPFLASAGGAAESGGAPEAGAAPGRLVNGGALTAFLLDGDLRLG
                     ATGTVTRVEADGSFVAFGHPFLGMGEIELPVAATDVVEVFASSMMSFKLAAPHAAAFR
                     LTRDRDPGVSGRMDRVAPMIPVRFRLEAGGAAKELRWSVAPAPALFPLLLAMSTDVAL
                     VLNDPTPAQKTIALRVSIGTAAGAFVYEDILSGPRAREIAILTAIALGGLVSDNEFED
                     PKISGVEISLANAPGERRFRVVNAGVSARRVAPGHRAGQRLGEAPLLD"
BASE COUNT          817 a         1904 c         2143 g          916 t
ORIGIN      
        1 cgagcttgcc ggcgagcgac tgcatctcgc gcgtcagctc gtcgaacttc gccccgaggt
       61 cggcgttcga gcgcaggagc gtggccgact gcttcgcgag cgaatcgttg agcttcgcca
      121 tctcgtcctt ggaggacgac tgcttctgaa gggtgtcgac ctgcttctcg acgacgctga
      181 cgtgcttgtg cagcccgtcg atgtcggtgc cgctcacgca tcctgccgcg gccgcgagca
      241 ggcccgcggc cgcgagcgtc ccgagcctcc tcacgcgcgt cacttcgcgg tcacgacgaa
      301 gtgtccgcgg cggttcttcg cccacgagtc ctcgtcgtgt ccggtcgcga aggggcgctc
      361 cttgccgtac gacaccgtct tgacgcggcc ggcgtccacg ccgagcgaga cgaggtactc
      421 cttggcggag ttcgcacggc ggtcgccgag cgcgaggttg tactgcgcgg tcccgcgctc
      481 gtcgcaatgg ccctcgatcg tgaacaggat cgtcgggtgg gacttgagcc acgccgcgtt
      541 ggcggcgagg acgtcgcgcg cgtcgctgcg gaggtcggcc ttgtcgtagt cgaagaacac
      601 gggcttcagg tagctcgaaa cgcgatcgag cgacatcgcc ttgatgtcgt cctcctgcga
      661 gcgcggggtc ggcttcggcg tcggggccgg agtcggctcc agggtgacga tcgtcggcgt
      721 cggcgtcgcc gccgggggag gaggcggcgg ctcgggagcg ggcttcgggg agcaggccac
      781 gacgagcgcc acggccgcga gcgcgaggga tccggacttc aagaggttct tcatggggcg
      841 agtccttcca ttgtgagaga agaggtgcga tcgatttccg gaggcgcaac gcccgcctcc
      901 gggggagagg tttctattct agcgtcttga tccacgcggg cgaataattg ttccccgccg
      961 acgtcacgat gcgcatcgcg gaaccgtccg cgttcgcgac cgcgatctgc gtcgagctgc
     1021 cccgcgtgac ttcccacgcc accttcgtgc cgtccggcga ccacgtgggg ttctcgtgcg
     1081 cgccggggcc ctcggagatc tgcagggtgc gccccgttcc gatctccatg acgcagatct
     1141 ggaagtcgcc ctcgttgcga cacgcgaagg cgatccgtcc gccgtccggc gagaaggcgg
     1201 cctcgtcgtt ccagctcccg gcgagcgaga cgcggcggac gttggcgccc tcgagatcca
     1261 tcatgtagat ctgcggcgag ccctggcggt tcgacgtgaa ggcgagctcg cggccgttcg
     1321 gggaccagcg cggcgtcgag tcgatcgcca cgttctcggt gagcctcttc aggttcgagc
     1381 cgtcggtctg cacggtgtag atctcggggt tccccttcac gctcccgcag aacgcgatcg
     1441 tctttccgtc gggcgaaaac gacggggacg cgttgagctg cgtggagaga ggcacacgga
     1501 acttgtcggt tccgtcccgg ccgacgagga acacgcccgg gttgtccttc tcgtaggact
     1561 ggtagacgat cttcttcccg tccggggacc agtccggagc gagcgagagc gtgcgcgagt
     1621 tcgtgacccg gcgctgggtc tcgccgtcga agtccatcgt gtagatctct ttcacgcgcg
     1681 gctcgtcgcg gtcggagacg aaggcgatct tcgtgaggta cggcccgggc ttcccggtga
     1741 actggcgcgt gaggtcgttc gcgagcgtgt gcgcgatgcg ccgggaggcg gcgagcgggc
     1801 ccgaatacat tttcgcgagg atcggctgga gggtgccgag atcgtagagc tgcgcctcga
     1861 cgacgatgct cgcgtcggtt tcctgggcga gccgcgtgtc gaggaggtac tgcgccgagg
     1921 aggccttcca ctggtcgcct tccgagcggt tgcgcggcgg gcggaggccc ttcgggtaga
     1981 gggtgggatc cgagacgagg aagacgcgcg agcccgacag gtcgtcgttg agcgtcgtga
     2041 agaacgggtc gacggcgccc ttctggagct ccggcttgag ggaggcgatc gtgacgggga
     2101 tcgcgagagg gatccgcgcg cccttggccc cgaggatgtc gacgacgacc tggctcggca
     2161 acgacgaaac aggcggcgcg acggggccgg tgccgggctt gccgttggcc ggggtcggct
     2221 gggcggaggc gcccgccgcg gctgcggcga ggacgagagc ggccagggtg cggatcggtg
     2281 tcggacggtt cacgaagcct cccatgacag gttctcagcg gaatcgcagg tggacgccga
     2341 gctgctcgtt tcggtagtcg ttcggcagcg gcggaagcgg gttggcggcg tagacggcgc
     2401 ggacggcggc gcggtcgtaa tagctcgccc cggagccgga ctcgatcttc acgtcggaga
     2461 tctggcccga ccgctggatg cggaaggaca cggtgcagag cgacccgtcg ggggcgtccg
     2521 gacggaacca gttcgcgcca atgagcgaga cgagctgctg gacgtagtag gagaagggaa
     2581 aatcggcgtc gaaggcggcc acgtcggcgc cgaagctcag ggccgcgccc gtcccgccgg
     2641 cggtcccgct cgggagcccc ggtgcgctcg gaagatcgac cgcgggcgcg ccctttcccg
     2701 gagccgccgc gggcttcggg ggcgtcggtt tcgctttcgc cggtttgagg gacggcatcg
     2761 cgttcggcga cggctcgatc ttctcgacct gcttcttctc gatcaccggc cgggacttca
     2821 cgggctcggg tgccggctcc ggaaccggga cggccgccac cggagccggc gcggcctccg
     2881 gccgtccgag ggcggccggg ctgacaacgc ggaccgggag cgaaatcgga atcgcggcgg
     2941 gaacgtgccg tggacggctc gtgaggatca ccgccgcgac gaaggcggcg tgggcgagga
     3001 tcgagaggcc cgccgagagg ggggcggggg ccctcccgcc gcgcgcgcgc cgcgcgagga
     3061 tctccgaaac cgtgagcggc gcgccgacgg ccgtcgtgtt cacggtcacg ccgtcgcccc
     3121 tgtcaacgac gatcctccga cttcagctcc gtgaccatgc cgagactctc cacccccagc
     3181 cggttgagcg tgtccatcat ttcgaccacg gaaccatacg gaaggttccg gtcggccttg
     3241 aggtagacgg acttgtcccg ccgccgggag aggacgagct ggagacgatc cttgagctgc
     3301 gacttggcga cgggcgtctc gttgaggtag tagttcccgc tcttcgtgat cgagaggatc
     3361 agggggtcct cgggggtctt cggcaggttc tgggcgacgg acttgggcag ctgcacttcc
     3421 actccctggt ggaggagtgg cgccgtgacc atgaagatca cgaggaggac gagcatgacg
     3481 tccacgagcg gcgtcacgtt gatgtcgtgg aacgagtcgg cttcgtcgtc gagcttgaag
     3541 gccacgggtg cctcaggtga agtggcgctc ggccaggttc atgaactcga gcgcgaagtc
     3601 ccgcatctcg ccgcgggccg cgcggacgcg cgcgttgaag ttgttgtacg cgatgagggc
     3661 cggaacggcc gcgacgaggc ccgcggccgt gttgatgagc gcctccgcga tgcctggcgc
     3721 cacgacgacg atcgacgtcg agcccttcga ggcgatctgc gagaacgaga tcaggatgcc
     3781 catgaccgtg ccgaaaaggc cgatgaacgg cgtcgccgag gccgtcgtcg cgaggaacga
     3841 gagcccccgc gtgaggcgcg tcgtctcgac gcccgcggcg cgctccagcg cgcgttcgat
     3901 ggaggcgagg ctcttcacga agagccggcc cgtgtccgcg ggcgcgacct cccggccgtc
     3961 cttgacctgc tggtcgagct cggcgtaacc cgcgcggaag atcccgacga gcgggctggg
     4021 ctcgcaccgg tcgcagatcc cgaggacgtc ggcgaacttg cggctcttcc ggaagtgcgc
     4081 gaggaacgcg cgcgaactcg cgcccgcgcg tgagaaactc cagatcttgc cgatgatgat
     4141 cgcccacgac acgagcgaga agaacgcgag cacgacgagg acggccttgg ccgttggtcc
     4201 ggccgtgagg aacagctgga ccaggttcga tccgttcgat tcgggcatga gtgttctttc
     4261 ggcctccctc gaaaaagggg gatggaatcc gcgacggtcc ggtcgccggc cgtcccgtca
     4321 aggtagcacg gaaactcgaa cggggtcaaa gcgttgggcg gcttttgcag ccctcgccct
     4381 ggccttcgcg gaggccgtcc acgcggcgcc ggcggcgctg ccgccggacg tcctcccgct
     4441 gtcggccgtc cgcgccggaa tgaagggctg gggcctcacc gtgatgaagg gcgccgtgcc
     4501 ggagcgcttc gaggtggagg tgctcggcgt cctcccgaac acgctcgggc ggagccggat
     4561 tctcgtgaaa gtctccgggc tcggcctcga gaggaccggc gtcatcgcgg gcatgagcgg
     4621 cagcccggtc tatctcgacg gcaagctggc gggagccgtt tcggcgacgt gggcgttcgg
     4681 caaggacccg gtcgggcagg tgacgccgat cgagaacatg ctcgaaggag gatcgggggg
     4741 cgcatcgccc gacgcgcccc gcgcccgggc cacggtctcg tccgcggccg ccttcgacgc
     4801 gctcgcatcc gcccacggcc ttccgcccga cgagcgcgtg gccgccctcg agaagatcgc
     4861 ggcgccctac cggccggcgg ccggccgcga ggccgcctcc ctcctcgcgc cggtctcggc
     4921 cgggcttccg tccgcgacgc tctcgcgctt cgccgaggat ctcggacggc tcggccttcc
     4981 ggccccgttc ctcgcgtccg cgggaggagc ggcggagtcc ggcggcgcgc cggaagcggg
     5041 ggccgctccg ggacggctcg taaacggagg cgcgctcacg gcgttcctcc tggacggcga
     5101 cctccggctg ggcgcgacgg gtacggtcac gcgcgtcgaa gcggacggga gcttcgtggc
     5161 gttcgggcat ccgttcctcg ggatggggga gatcgaactc ccggtcgccg ccaccgacgt
     5221 cgtcgaggtc ttcgcgagct cgatgatgtc gttcaagctc gcggcgccgc acgcggcggc
     5281 gttccggctc acgcgcgacc gcgacccggg cgtctcgggc cggatggacc gcgtcgcgcc
     5341 catgatcccc gttcgcttcc gcctggaagc cggcggcgcg gcgaaggagc tgcgctggag
     5401 cgtggcgccc gcccccgcgc tgttccctct ccttctcgcg atgtcgacgg acgtggcgct
     5461 cgtcctgaac gacccgacgc ccgcgcagaa gaccatcgcg ctgcgcgtct cgatcggcac
     5521 ggcggcggga gcgttcgtct acgaggacat cctgtccggc ccgcgcgcgc gagagatcgc
     5581 gatcctcacc gcgatcgcgc tcgggggcct cgtctccgac aacgagttcg aggacccgaa
     5641 gatctccggc gtggagattt ccctcgcgaa cgcgccgggc gagcgccgct tccgcgtcgt
     5701 gaacgcgggc gtgtccgcgc gccgcgtcgc gcccggccac cgtgcgggcc agcgtctcgg
     5761 cgaggcgccg ctccttgatc
//
LOCUS       DAOMFY010000253         5762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4738_length_5762_cov_6.720694, whole genome shotgun sequence.
ACCESSION   DAOMFY010000253 DAOMFY010000000
VERSION     DAOMFY010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4738_length_5762_cov_6.720694"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLB01_16015"
     CDS             complement(<1..276)
                     /locus_tag="PLB01_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78860.1"
                     /translation="MRPSAKVAAAVLGLAILAALLAPLLPSAPATRIDLTRRLVPPAS
                     APPLGTDELGRDLFSRLLRGATVSLGVAGAAVLASAAMGSTLGLVAGE"
     gene            complement(273..1205)
                     /locus_tag="PLB01_16020"
     CDS             complement(273..1205)
                     /locus_tag="PLB01_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78861.1"
                     /translation="MPPLRRLLEVPLLLLGAATVVFFLIHLIPGDPAAAMLGEGAAPA
                     DVAGLRKDLGLDRPLLGQYVSWLAGAVRGDLGSSILTKRKVTREILAAVPATAELALA
                     AFLGALLVAVPTGVASALARGKPLDGVLRAVSLLGVSTPNYVSGPLLVLVFSIGLGLL
                     PVSGRDGRGALVLPAATLAFALAALLSRMVRACLADEMDRPYVAAAIARGERPLRAIL
                     RHAGRNALPPVLVVAGLQFGSLLTGAVVTETIFSWPGVGRLLVQSIQRRDYPLVQGCV
                     LFIAVTFILVNASVDALAAALDPRARARAAGEST"
     gene            1355..2176
                     /locus_tag="PLB01_16025"
     CDS             1355..2176
                     /locus_tag="PLB01_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78862.1"
                     /translation="MRHVFLHAALVLNLATGVASAAEPAEQQRRPLGEESRPASRDAL
                     ATPHAQAVGAVSHLLLPTSANVNGQFGSVYKTKVSIFNAVNATYNIRAGLSVSNGEIS
                     HTFIQIGPGQTLTFNNFLSDVFGYYGGGAVDLDSGNTNLKFVVNSQVYVDTVNGRYTT
                     AVQFADDLGAIIPSRPGYVVGVSVNSVRRTNIGCASNSSLDQTITFQAFDANNFAIGN
                     PFSFTLAGYGWAQYSYTGSLTNGGIYITATRNAVCFGVEVDNTSNDGTYQLATPF"
     gene            complement(2259..5063)
                     /locus_tag="PLB01_16030"
     CDS             complement(2259..5063)
                     /locus_tag="PLB01_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ78863.1"
                     /translation="MRGFRRFAFVLLCALAASRATGQTTSSIVGRVADDAGVPLPGVT
                     VEATSPALQGSGRAVTDAQGRYRLSLLPPGTYTVTFAISGFASEKQTNVVVALGRDTS
                     LSADLHLATKVQTSVTSEASAIELTSSGVGTSLDQRAVTTLPTGRNYSSVAQVVPGVS
                     SDANPGNTEQATITVYGSSGAENSFYIDGVNTTGAEYGFQGKELNFEFIQAVDVKTGG
                     YEAEFGRSTGGIVNVITKSGGNDFHGDVFGYYDGDNLQANAEPVVSNTTSAGYTRKDF
                     GIGIGGYILKDRLWFFGAYDRVDNTQDSTLPEGPRAGQVARSQSTRDLGSGKLTFKAS
                     QNQTVTGTVFLDPRDDTGAINDATHSLNGDPLTYDGKKSFGGTDYALRYEGLFGARWL
                     FTLQGARHQEKNEVGPASAAGDVIQYRDVQNNFYQTGGFGLIQNKSFTRDMLTFSATA
                     FLGAHEAKFGVEYERESADVLKRYSGGQQVDVFANPENPAKPVYSHFYWTTPNATVAN
                     APVSALNASPEHKVTTLYLQDKWTVLPNLTVNIGLRWDRQEIVDASGVTQIDLKDDFA
                     PRFGFAWDATKDAKTKIYGSYGRYYEQLSMDLVIRSFSYERQPRIFNYSPTSTTPDPA
                     AEADLGQTSNILGGFTEPSDPNLRNQYIHEYVFGAEREVAKNVVVGIKGIYRNYGEVI
                     EDFLCADDGTYCIGNPGKGIMERLYTLDYSQTFASPEARRTYKAVQLDVTKSFADSWW
                     GQASYIYSKLEGNFDGLYSPFTNVGADPNISAAFDYYDFFTDGKNLKNITNDGPLSND
                     RRHQFKVSGYYVFPFGLSVGAAGYYRSGTPVTRYGYSDAYGRYEFFMTPRGAEGRAPD
                     NYDLDLHLGYPIALGPVKVNVLLDVFNLFNTQKAILLDQRYGFQEDDNSAAKPVNRDY
                     LQPVLRTPPTTVRLGARVSF"
     gene            complement(5160..>5762)
                     /locus_tag="PLB01_16035"
     CDS             complement(5160..>5762)
                     /locus_tag="PLB01_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQQ78864.1"
                     /translation="PDEWRAFIDAAEGDETLSAAAPLWRFKLLTASRISEMVGLRWSD
                     ISFERRVVAIRQKKTGTVKTLTLTPEMEKILKSVRPGIGAALVFPSPEGPAWGFFRLR
                     GHFHRTVRLAGLTGAWTPHSIRHTAATWARKAGTPLDRIAKTLGHAGLNLVERYAHFS
                     VEDLNPTLDAISAMERRGGERTVNENLTNIKTGLTASSTR"
BASE COUNT          962 a         1885 c         1992 g          923 t
ORIGIN      
        1 ctcgcccgcg acgagcccga gggtggagcc catcgccgcc gaggcgagga cggccgcgcc
       61 cgcgacgccg agggacaccg tcgcgccgcg caggagtcgc gagaagaggt cgcgcccgag
      121 ctcgtcggtc ccgagtggcg gtgcggacgc cggcgggacg agccgccggg tcaggtcgat
      181 ccgcgtcgcc ggcgcggaag ggaggagcgg tgcgagcagg gccgccagga tcgcgaggcc
      241 gaggacggcg gccgcgacct tcgcggacgg cctcacgtgc tctcccccgc cgcgcgggcg
      301 cgggcgcgcg ggtcgagcgc cgcggcgagc gcgtccacac tcgcgttcac gaggatgaac
      361 gtcacggcaa tgaacaggac gcagccctgc acgagcgggt agtcgcggcg ctggatcgac
      421 tggacgagaa ggcgtccgac cccgggccac gagaagatcg tctccgtcac gacggcgccc
      481 gtcaggagcg agccgaactg caggcccgcg acgacgagca cgggcgggag cgcgttgcgc
      541 cccgcgtggc ggaggatcgc gcggagcggg cgctcgccgc gcgcgatcgc ggccgcgacg
      601 tacgggcggt ccatctcgtc ggcgaggcac gcccgcacca tccgggacag gagcgccgcg
      661 agggcgaacg cgagcgtggc cgcggggagg acgagcgcgc cgcggccgtc gcgccccgac
      721 acgggaagga ggccgagacc gatcgaaaag acgaggacga ggagcggccc cgagacgtag
      781 ttcggggtcg agacgccgag gagcgacacg gcgcgcagga cgccgtccag gggcttgccg
      841 cgcgcgagcg ccgacgccac gcccgtcggg accgcgacga ggagcgcccc gaggaacgcg
      901 gcgagcgcga gctcggccgt cgcggggacg gcggcgagga tctcccgggt gaccttccgc
      961 ttcgtgagga tcgacgagcc gaggtccccg cgcacggcgc cggcgagcca cgacacgtac
     1021 tgtcccagga gcggccggtc gagaccgagg tctttccgca ggcccgccac gtccgccggc
     1081 gccgcgccct cgccgagcat cgcggcggcc gggtcgccgg ggatcaggtg gatcaggaag
     1141 aaaacgaccg tcgccgcgcc cagcaggagc agcgggacct cgagcagtcg gcggagcggc
     1201 ggcacgcgcg gagtctagcg tccgcgggcc aacgacggcg gcatcccggg ggatgagagg
     1261 gaagtccttg cggacggcgc gctcacgggc tgagaatcgc tgtgccacac cgcccgtcaa
     1321 cgccgcgaca ctctttgccg aacaggagga agacatgcgt cacgtgtttc ttcacgccgc
     1381 cctcgttctc aatctcgcaa cgggagttgc aagcgccgcg gagccggccg agcaacagcg
     1441 ccggcctctc ggcgaggaga gccgtcccgc ctcgagggac gcgctcgcca cgccgcacgc
     1501 ccaggccgtc ggagccgttt cacacctgct gctcccgacc tccgcgaacg tgaacggcca
     1561 gttcggctcc gtctacaaga cgaaggtcag catcttcaac gcggtgaacg ccacctacaa
     1621 catccgggcc gggctcagcg tctcgaacgg agagatctcg cacacgttca tccagatcgg
     1681 cccgggccag acgctgacct tcaacaactt cctctcggat gtcttcggct actacggcgg
     1741 cggcgcggtc gacctggaca gcggaaacac gaacctcaag ttcgtcgtca attcccaggt
     1801 atacgtggac acggtcaacg gacggtacac gaccgccgta cagttcgccg acgacctcgg
     1861 ggccatcatc ccgagccggc ccggttacgt cgtcggcgtc tccgtcaact cggtgcgacg
     1921 cacgaacatc gggtgtgcga gcaactcgag cctcgaccag accatcacgt tccaggcttt
     1981 cgatgcgaac aacttcgcca tcggaaaccc gttctccttc accctcgccg gctacgggtg
     2041 ggcccagtac tcgtacacgg gctccctgac gaacggcggc atctacatca cggcgacacg
     2101 gaacgccgtc tgcttcggag tcgaggtcga caacacgtcg aacgacggga cgtatcagct
     2161 cgcgacaccg ttctgacgtc cgccctgcgg ttcacgccgg agcgcgggag gcgggacaaa
     2221 gtcgccgccc gcgctccgga accacgacgc catgaccttc agaagctgac gcgcgcgccg
     2281 agccgcaccg tcgtcggcgg cgtccggagc acgggctgga ggtaatccct gttgacgggc
     2341 ttcgcggcag agttgtcgtc ctcctggaaa ccatagcgct ggtcgagaag gatcgccttc
     2401 tgcgtgttga agaggttgaa gacgtcgagg aggacgttga ccttcacggg cccgagcgcg
     2461 atcggatacc cgaggtggag gtcgaggtcg tagttgtcgg gcgcgcgtcc ctctgcacca
     2521 cgcggcgtca tgaagaactc gtagcggccg taggcgtccg agtagccgta gcgcgtgacc
     2581 ggcgtcccgg accggtaata ccccgcggcg ccgacggaaa ggccgaacgg gaagacgtag
     2641 tagcccgaga ctttgaactg gtggcgccgg tcgttcgaga gcggcccgtc gttcgtgatg
     2701 ttcttgaggt tctttccgtc ggtgaagaag tcgtaatagt cgaaggcggc cgagatgttc
     2761 gggtcggcgc cgacgttcgt gaacggtgag tagaggccgt cgaaattgcc ctcgagtttc
     2821 gaatagatgt acgaggcctg gccccaccag ctgtccgcga agctcttcgt gacgtcgagc
     2881 tggacggctt tgtacgtccg tctcgcctca ggcgacgcga acgtttgcga gtagtcgagc
     2941 gtgtagagcc tctccatgat tcccttgccg gggttcccga tgcagtacgt cccgtcgtcc
     3001 gcgcagagga agtcctcgat gacctcgccg taattgcggt agatcccctt gatcccgacg
     3061 acgacgttct tcgcgacctc gcgctcggcc ccgaaaacgt actcgtggat gtactggttg
     3121 cgaaggttcg ggtcggacgg ttccgtgaag cccccgagaa tgttcgacgt ctggccgagg
     3181 tcggcctctg ccgccgggtc cggcgtcgtg gacgtcgggc tgtagttgaa gatgcgcggt
     3241 tgccgctcgt acgagaacga ccggatcacg aggtccatcg agagctgctc gtaataacgt
     3301 ccataagatc cgtagatctt cgtcttggcg tccttcgtcg catcccatgc gaacccgaag
     3361 cggggcgcga agtcgtcctt gaggtcgatc tgcgtgacgc ccgacgcgtc gacgatttcc
     3421 tgccggtccc accgcaggcc gatgttcacg gtgaggttcg ggaggaccgt ccacttgtcc
     3481 tgaaggtaga gcgtcgtgac cttgtgctcg gggctcgcgt tgagcgccga gaccggggcg
     3541 ttcgcgaccg tggcgttcgg cgtggtccag tagaaatggc tgtaaacggg cttggcgggg
     3601 ttctcggggt tcgcgaagac gtccacttgc tggccgcccg aataccgctt caggacgtcg
     3661 gcggactccc gctcgtattc gacgccgaat ttcgcctcgt gagctccgag gaaggccgtc
     3721 gccgagaacg tgagcatgtc ccgcgtgaag ctcttgttct ggatgaggcc gaagccgccg
     3781 gtctggtaga agttgttctg cacgtcgcgg tactggatga cgtcgcccgc ggcggaggcc
     3841 gggccgacct cgttcttctc ctggtgccgc gcgccctgca gcgtgaagag ccaccgcgcc
     3901 ccgaaaaggc cctcgtagcg gagggcgtag tcggtcccgc caaagctctt cttcccgtcg
     3961 taggtgaggg ggtcgccatt caacgagtgc gtggcgtcgt tgatcgctcc ggtgtcgtcg
     4021 cgcggatcga ggaagaccgt ccccgtcacg gtctggttct ggctggcctt gaacgtgagc
     4081 ttgccggacc cgaggtcacg cgtgctctgc gagcgcgcca cctgtcccgc ccgcggcccc
     4141 tccggaagcg tcgaatcctg cgtgttgtcg acgcggtcgt aggcgccgaa gaaccagagc
     4201 cggtccttga gaatgtatcc gccgataccg atgccgaagt ccttccgggt gtagcccgca
     4261 gacgtcgtgt tcgagacgac cggctccgcg ttcgcctgca ggttgtcgcc gtcgtagtag
     4321 ccgaagacgt cgccgtggaa atcgtttccg ccggacttcg tgatcacgtt cacgatgccg
     4381 cccgtcgagc gcccgaactc ggcctcgtag ccgcccgtct tgacgtccac ggcctggatg
     4441 aactcgaagt tgagctcctt gccctggaag ccgtattcgg cgccggtcgt gttcacaccg
     4501 tcgatgtaga acgagttctc cgcgccgctc gacccgtaga ccgtgatcgt ggcctgttcc
     4561 gtgtttccag gattcgcatc ggacgaaact cccggaacga cctgggcgac cgaggagtag
     4621 ttgcggcccg tcggaagggt cgtgacggca cgctggtcga ggctcgtccc gacgccggag
     4681 gacgtcagtt cgatcgcgga ggcttcgctc gtcaccgacg tctggacttt cgtcgcgagg
     4741 tgcaggtccg cggaaaggga cgtgtcgcgt ccgagggcca cgacgacgtt cgtctgcttt
     4801 tccgacgcga agcccgaaat ggcgaaggtc acggtgtacg tgcccggcgg gagaagcgac
     4861 aggcggtacc gcccctgcgc gtccgtcacg gcccgccccg agccctgcag ggcaggcgag
     4921 gtcgcctcga ccgtgacacc cgggagcggc accccggcgt cgtcggcgac gcggcccacg
     4981 atgctgctcg tggtctggcc cgtggcccgc gaggcggcca gggcgcacag gaggacgaag
     5041 gcgaagcggc ggaaaccacg catgggctcc tcctcgtcgg aactcaagaa aaaagaaccc
     5101 tagggtcggg gcactctagc acgctagaga gactcagcct cagcggatcc cgagcttctt
     5161 catctcgtag aacttgccgt caagccagtc ttgatgttcg tgagattttc gttcacggtt
     5221 cgttcaccgc cgcgccgttc catcgcagag atggcgtcca gagtcgggtt gagatcctct
     5281 acggagaagt gcgcataccg ctcgacgagg ttgaggccgg cgtgccccag ggtcttcgca
     5341 atccggtcga ggggcgtccc tgccttcctc gcccacgttg cggccgtgtg ccgaatgctg
     5401 tgcggcgtcc aggcgcccgt caggccggcg aggcggacag ttcggtggaa gtgcccgcgg
     5461 aggcggaaga agccccacgc aggtccctcc gggctcggga agaccagggc ggcgccgatg
     5521 cccgggcgaa cagacttcag gatcttctcc atctcggggg taagggtgag ggtcttgact
     5581 gtgccggtct tcttctggcg aatcgcaacg acccgacgct cgaagctgat gtcggaccag
     5641 cgcagaccca ccatctcgga gatgcggctc gcggtaagga gcttgaagcg ccagagaggg
     5701 gcggccgcac taagcgtctc gtctccctct gcggcatcga tgaatgccct ccactcgtcc
     5761 gg
//
LOCUS       DAOMFY010000254         5749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4760_length_5749_cov_7.529856, whole genome shotgun sequence.
ACCESSION   DAOMFY010000254 DAOMFY010000000
VERSION     DAOMFY010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4760_length_5749_cov_7.529856"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLB01_16040"
     CDS             <1..389
                     /locus_tag="PLB01_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78865.1"
                     /translation="LLFGASSSGDPLFLRLEPGAAGASALVGLTLRVELLVPGADEPA
                     AFEAPIGSGITDASGARIALGKALEARLPLGVPHAGPLVFRVTVLDASGRVAEAIPAD
                     GWLRFTPPGPASSAMSLLKLRAGPPS"
     gene            414..1295
                     /locus_tag="PLB01_16045"
     CDS             414..1295
                     /locus_tag="PLB01_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HQQ78866.1"
                     /translation="MCRFALYVGPPITVDSLTTRPEHSILKQSLHAEEREDPTNGDGF
                     GVGWYVPELGPEPALYRSPVPAWNDPNLLHISRVSKSPCILAHVRAASPGTAVSVSNC
                     HPFASGPFAFMHNGSIGGFARIRRRLEAGLSEDAFAGIQGTTDSELAFALFLDAYAQV
                     KAEDATERMVGALTEAVAKIEWTADDAGVTEPSYLNFAVTDGRRAVVMRYATPPMVPA
                     SLWGSHGRRFVCEGGACRMSEPGKDGAATLVCSEPLSLDGDWQKVPAQNMVVIRGGGH
                     AELLPIPFNDLTRSVAG"
     gene            1338..2933
                     /locus_tag="PLB01_16050"
     CDS             1338..2933
                     /locus_tag="PLB01_16050"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HQQ78867.1"
                     /translation="MRSPNTLVLVLHGHIPDVLGHGTWPHGANWLYEAAAETYLPFLR
                     VAERLLDRGVPIKATVGMTPILCEQLSDARFGPGFDAYLAQRAAAARKDLETLGALGD
                     AGAVSVATFWATFYEEARADFAAREGGLLPAFRNLADRGALEMIASAATHGFLPLLPN
                     DRAIERQLDVGLAAHARHFGRPARGIWLPECAYRPAGAWVSPADGHIEERRGLEDFLS
                     PRGVGFFFVETHLVKGGRHEPAYGGVVETPEGGSTPYRIHAVRTSDGGRVGVLARDPL
                     SSQQVWSGKVGYPGDGRYLEFHKKRAPSGHRYWRVTDSRLDLGDKLPYEPAAIPAALD
                     AHATHFVHLLERAPTLPGGEGATVVAMFDFELFGHWWFEGVEFLAAVFEKLAGRAGVV
                     PRTASEALAATRPEGIRMPAGTWGRGGDFQVWWNDHTVPYWREVAATELAIERFEEAR
                     DAIPPRLFAALERQALLLQSSDWPFLIDNEVSRDYADARIVGHVRDFWRLARMADGGA
                     SDDAFLDELDDRDRLFAPELEGR"
     gene            2988..5531
                     /locus_tag="PLB01_16055"
     CDS             2988..5531
                     /locus_tag="PLB01_16055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQQ78868.1"
                     /translation="MAATPSGAPWMVAILEEQRRRGHDVSAVIAGRDGTLAPILAGLG
                     IPFEIFPFGLFGRGVLGASLQGLRLVRLLRRLRADVVQTHLFPSIVLGRIASWAADVP
                     LRYSMIPGPYYLECPVLGDLEAGTAWADTRVIASCERTRELYMEKGVPRERVDLVYYG
                     VDPARFDPARADGGRVRRELGISPGIPVVGLVAYFYPKVPDAPTSPTRFVGLGIKGHD
                     VLLAAVPEVLRHVPDAVFLLVGGGWGAAGDELLAETRALAERLGVAHAVRFTGERRDV
                     ADVLAALDVSLQCSRSENLGGAIEALLMARPLVVTRVGGLVDAVKDGKTGLVVPPDDA
                     ASLAAAIVLLLRDRDLAGRLGDAGRSLMAERFSLARTVDDLERIYHEDAAAAGNGQLR
                     GYRPSVTAARLAAVPFRSLPALARARRAWRAPRRTTAGDAPLPPPRTASGVPSSRVAL
                     VVGVPVGSSWLVDHARGLKARGWDVVVTVDSGPGGLAPLVESAGVPVRRLPLWVGPKR
                     LRALTWLAKFPGAARALARQFLKDGVTLVVTHQFTSILIARAAARIAGIPHVSMVPGP
                     RHLEALATRVADRLTWRTDAATVAGCRHTRDLYVALGRDPRRLETIYYGLDPDAVRPV
                     RARADVRRGLGLADDAEVVVHVAHFYPPITGVQVPGHTRGLDLKGRGDLLDAARALFA
                     RRPRARLLFVGGGLDDGGEAYRGWLADRCASDPALAGRVLFTGHRDDVADLLAASDVA
                     VQCSLTENLGGTLESLVLARPTVATRVGGMPESVKHGLSGLLVPPSDPRALAAAIERL
                     LENPAEAEAMGRAGRTLVLERFTLSRTLDELSRLFVRLGQTPSRPTEAA"
     gene            5528..>5749
                     /locus_tag="PLB01_16060"
     CDS             5528..>5749
                     /locus_tag="PLB01_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78869.1"
                     /translation="MSDGPHETWDSAARPPRAAEEWRELWRHRSLVGALVARNVKVRY
                     KRSLLGVAWSMLAPAGMMIVLSLVFSQVFR"
BASE COUNT          686 a         2195 c         2007 g          861 t
ORIGIN      
        1 agcttctctt cggcgcgtcc tcgtcgggcg accccctctt cctgcgcctc gagcccggcg
       61 cagcgggcgc gtccgccctc gtcggcctca cgctgcgcgt ggagcttctc gtccccgggg
      121 cggacgagcc cgccgcgttc gaggcgccga tcggttccgg gatcacggac gcgagcggcg
      181 cgcggatcgc gctcggcaag gcgctcgagg cgcgccttcc gctcggcgtc ccgcacgccg
      241 gccctctcgt tttccgggtc acggtcctcg acgcgagcgg ccgagtcgcc gaggcgatcc
      301 cggccgacgg atggctgcgc ttcacgccgc cgggccccgc gtcgtcggcg atgagcctcc
      361 tcaagctgag agccgggccg ccgtcctgag gctccccggc gtacgatgcc cgcatgtgcc
      421 gattcgccct ctacgtcggg ccgccgatca cggtcgactc cctgacgacg cgtcccgagc
      481 actcgatcct caagcagagc ctgcacgccg aggagcgcga ggacccgacg aacggcgacg
      541 gcttcggagt cggctggtac gtccccgagc tcgggcccga gccggcgctc taccgctcgc
      601 ccgtccccgc gtggaacgac ccgaacctcc tccacatctc gcgcgtctcg aagagcccgt
      661 gcatcctcgc gcacgtgcgc gccgcgtcgc ccgggacggc cgtctccgtc tcgaactgcc
      721 acccgttcgc gtcgggcccg ttcgcgttca tgcacaacgg ctcgatcggc gggttcgccc
      781 gcatccgccg gcgcctcgag gcgggtctct ccgaggacgc cttcgcgggc atccagggca
      841 cgacggactc cgagctcgcg ttcgcgctct tcctcgatgc gtacgcgcag gtgaaggccg
      901 aggacgcgac ggagcgcatg gtgggcgctc tcacggaagc cgtcgcgaag atcgaatgga
      961 ccgccgacga cgcgggcgtg acggagccgt cgtacctgaa cttcgccgtc acggacggcc
     1021 ggcgcgccgt cgtcatgcgg tacgcgacgc cgccgatggt gcccgcgagc ctctggggct
     1081 cccacggccg ccggttcgtc tgcgagggcg gcgcctgccg catgagcgaa cccggcaagg
     1141 acggcgccgc gacgctcgtg tgctccgagc cgctctctct cgacggcgac tggcagaagg
     1201 tccccgcgca gaacatggtc gtgatccgcg gcggcggaca cgcggagctt ctcccgatcc
     1261 cgttcaacga cctcacgcgc agcgtcgcgg gctgagagcg gatcgcgatt gcgcgcagcg
     1321 tagaatcgcc gcccttcatg cgctcgccga acacgctcgt cctcgtcctg cacggccaca
     1381 tccccgacgt cctcgggcac ggcacgtggc cgcacggcgc gaactggctc tacgaggccg
     1441 ccgccgagac gtacctcccg ttcctgcgcg tggccgagcg gcttctcgac cgcggcgtgc
     1501 cgatcaaggc gaccgtcggg atgacgccga tcctctgcga gcagctctcg gacgcccgct
     1561 tcggcccggg attcgacgcc tacctcgcgc agcgcgcggc cgccgcccgg aaggacctcg
     1621 agaccctcgg ggcgctcggc gacgcgggcg ccgtctccgt cgcgacgttc tgggcgacct
     1681 tctacgagga ggcgcgcgcg gacttcgcgg cgcgagaggg cggccttctc ccggcgttcc
     1741 ggaatctcgc cgaccgcggc gcgctcgaga tgatcgcctc ggccgcgacg cacggctttc
     1801 tcccgctgct cccgaacgac cgcgcgatcg aacgccagct ggacgtcggc ctcgcggcgc
     1861 acgcgcggca tttcggccgc ccggcgcgcg ggatctggct gcccgagtgc gcgtaccggc
     1921 ccgcgggcgc ctgggtctcc cccgcggacg ggcacatcga ggagcgccgc ggcctcgagg
     1981 acttcctctc gccgcgcggc gtcggcttct tcttcgtcga gacgcacctc gtgaagggcg
     2041 ggcggcacga gcccgcgtac ggcggcgtgg tcgagacgcc cgagggcggt tcgaccccgt
     2101 accggatcca cgcggtccgc acgtcggacg gcggccgtgt cggcgtcctc gcacgcgacc
     2161 cgctctcctc gcagcaggtc tggtccggga aggtcggcta ccccggagac ggccgctacc
     2221 tcgagttcca caagaaacgc gcgccgagcg gccaccgcta ctggcgcgtc acggattcgc
     2281 gtctcgacct cggcgacaag ctcccctacg agccggccgc gatcccggcg gcgctggacg
     2341 cgcacgcgac gcacttcgtc cacctgctcg agcgcgcacc cacgctgccg ggcggcgaag
     2401 gcgccaccgt cgtcgcgatg ttcgacttcg agctgttcgg ccactggtgg ttcgagggcg
     2461 tcgaattcct cgcggcagtc ttcgagaagc tggccgggcg ggccggcgtc gtgccgcgca
     2521 cggcgagcga agccctcgcc gcgacccgcc ccgagggcat ccgcatgccg gccggcacgt
     2581 ggggacgcgg aggggacttc caggtgtggt ggaacgacca caccgtgccc tactggcggg
     2641 aggtcgccgc cacggagctc gcgatcgaga ggttcgagga agcacgtgac gcgatcccgc
     2701 cccgcctctt cgccgcactc gaacgccagg cgctgctcct gcagtcgagc gactggccgt
     2761 ttctcatcga caacgaggtc tcgcgcgact acgccgacgc gcgcatcgtc ggccacgtcc
     2821 gcgacttctg gcggctcgcg cggatggccg acggcggggc gtcggacgac gcgttcctcg
     2881 acgagctcga cgaccgcgac cgcctgttcg cgccggagct cgagggccgc tgagcttcgg
     2941 gtagtctcgg gcaggtgcac ccggaacggc cgcttcggat cgtccacatg gccgcgacgc
     3001 ccagcggcgc gccgtggatg gtcgcgatcc tcgaggagca gcgccgccgt ggccacgacg
     3061 tctcggccgt gatcgccggc cgggacggca cgctcgcgcc gatcctcgcg ggcctcggga
     3121 tcccgttcga gatctttccg ttcggccttt tcggaagggg cgtcctcggc gcgtcgcttc
     3181 agggcctgcg cctcgtgcgc ctcctgcgcc gcctccgcgc ggacgtcgtc cagacccacc
     3241 tcttcccgtc catcgttctc gggcgcatcg cgtcatgggc tgcggacgtg cccctgcggt
     3301 actcgatgat cccgggcccc tactacctcg agtgcccggt tctcggcgac ctcgaggcgg
     3361 gcacggcgtg ggccgacacg cgcgtgatcg cgtcgtgcga gagaacgcgc gagctctaca
     3421 tggagaaagg cgtgccgcgc gaacgcgtcg acctcgtcta ctacggagtc gacccggccc
     3481 gcttcgaccc ggcgcgggca gacggcgggc gcgtgcggcg cgaactcggc atctcccccg
     3541 ggattccggt cgtcgggctg gtcgcctact tctacccgaa ggtcccggac gcgccgacgt
     3601 cgccgacccg gttcgtcgga ctcgggatca agggacacga cgtcctcctc gccgcggtcc
     3661 cggaggtcct gcgccacgtt cctgacgcgg tcttcctcct cgtgggcgga ggatgggggg
     3721 ccgctggcga tgagctgctt gccgaaacgc gcgcgctcgc ggagcgcctc ggtgtcgcac
     3781 acgccgtccg cttcaccggg gagcggcgag atgtcgcgga cgtccttgcg gcgctcgacg
     3841 tctcgctcca gtgctcgcgg agcgagaacc tcggcggcgc gatcgaggcc ctcctcatgg
     3901 cccggccgct cgtcgtgacg cgcgtcggcg gcctcgtcga cgccgtgaag gacgggaaga
     3961 ccggtctcgt ggtgccgccc gacgatgccg cctcgctcgc cgcggcgatc gtcctcctcc
     4021 tgcgcgaccg tgacctcgcc gggcgcctcg gggacgccgg ccgctcgttg atggcggaac
     4081 gattcagcct cgcgcggacg gtcgacgacc tcgagcgcat ctaccatgag gacgcggccg
     4141 cggccgggaa cggacagctt cggggctacc gcccgtccgt gaccgccgcg cgcctcgcgg
     4201 cggtgccctt ccgctcgctc ccggccctcg cgcgtgcgcg ccgggcatgg agagcgcccc
     4261 gccgaacgac cgccggcgac gctccgcttc ccccgccgcg gactgcctcc ggagtcccgt
     4321 cttcccgcgt cgctctcgtc gtcggcgtgc cggtcggctc ctcctggctc gtggaccacg
     4381 cgcgtggatt gaaggcccgc gggtgggacg tcgtcgtcac cgtcgattcg ggccccggcg
     4441 gcctcgcgcc gctcgtcgag tcggccggcg ttccggtccg gcgcctgccc ctctgggtcg
     4501 ggccgaagag gctgcgcgcg ttgacctggc tcgcgaagtt tcccggtgcg gcccgggcac
     4561 tcgcgcgcca gtttctgaaa gacggcgtga cgctcgtcgt cacgcaccag ttcacgtcga
     4621 tcctgattgc gcgcgccgcg gcaaggattg ccgggatccc acacgtctcg atggtcccgg
     4681 gcccgcgaca cctggaggcg ctcgccacgc gcgtcgccga tcgcctgacg tggcggacgg
     4741 acgcggcgac ggtcgccggc tgccggcaca cccgcgatct ctacgtcgcg ctcggccgcg
     4801 acccgaggcg gctcgagacc atttactacg gcctcgaccc cgatgcggtg cgacccgtgc
     4861 gcgcgcgcgc cgacgtgcgg cgcgggctcg gcctggctga cgacgccgag gtcgtcgttc
     4921 acgtcgccca cttctacccc ccgatcaccg gcgttcaggt cccgggacat acgcgcggtc
     4981 tcgatctcaa gggtcgtggg gacctgctcg acgcggcgcg cgccttgttc gcaaggcgtc
     5041 cccgggcgcg gctgcttttc gtcggcggcg gcctggacga tggcggcgag gcctaccgcg
     5101 gctggctcgc ggaccgctgc gcctcagacc ccgcgctcgc gggccgcgtc ctcttcaccg
     5161 gtcaccggga cgacgtcgca gacctcctcg ccgcctcgga cgtggcggtc cagtgctcgc
     5221 tcacggagaa cctcgggggg acgctcgagt cgctcgtcct cgcgcgtccc acggtggcca
     5281 cgcgggtcgg cggaatgccc gagagcgtga agcacggcct gagcggcctc ctcgtcccgc
     5341 cttccgaccc gagggccctc gcggccgcga tcgagaggct tctcgagaat ccggccgaag
     5401 ccgaggcgat ggggcgggcc gggcggacgc tcgtcctcga gcgcttcacg ctttcccgca
     5461 cgctcgacga gctctcccgt ctgttcgtgc ggctcgggca gacgccctcg cgccccacgg
     5521 aggcggcttg agcgacggcc ctcacgagac gtgggactcg gcggcgcgcc ctccgcgcgc
     5581 cgccgaggaa tggcgcgagc tgtggcgcca tcggagcctc gtcggcgccc tcgtcgcacg
     5641 caacgtgaag gtccgttaca agcggtccct tctcggagtc gcgtggtcga tgctcgcccc
     5701 cgccgggatg atgatcgtcc tctcgctcgt cttctcgcag gtcttccgg
//
LOCUS       DAOMFY010000255         5727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4789_length_5727_cov_8.320874, whole genome shotgun sequence.
ACCESSION   DAOMFY010000255 DAOMFY010000000
VERSION     DAOMFY010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4789_length_5727_cov_8.320874"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLB01_16065"
     CDS             complement(<1..366)
                     /locus_tag="PLB01_16065"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HQQ78870.1"
                     /translation="MAYAVRRPVENVYLVRERDRHRSRELFGFALAAVPPVIVLFVAI
                     WANVKTVELGYRIATVAKRREALLETKRKLEMDRAQAAALSRVEDVSRRTLGLVPPTL
                     DQVVLVKDAALAPAPPPARP"
     gene            complement(376..1344)
                     /gene="rsmH"
                     /locus_tag="PLB01_16070"
     CDS             complement(376..1344)
                     /gene="rsmH"
                     /locus_tag="PLB01_16070"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQQ78871.1"
                     /translation="MEGERAGHVPVLLREVVDALRPEGGGVFVDATLGLGGHAEALLE
                     ASPLVRVVGIDRDPEALVLARERLARFGDRLVTVLGRHEDVAAHLDALGIGRVAGVLA
                     DLGVSSMQIDRAERGFSFMKDGPLDMRMGPEGPTAADLVATASRDELTRIFREWGEER
                     MAGPIARRVVEARETAPIRTTGEMRRLVLRALGPRREKHKDPATRVFQALRIATNREL
                     VELERFLDDAIARLALGARLSVLSYHSLEDRIVKRAFQRHTAGCTCPPSFPVCVCSRR
                     RVMALVTKKAIRPTLAELGENPRARSARLRVLERVAEETGPLTPPR"
     gene            complement(1354..1800)
                     /locus_tag="PLB01_16075"
     CDS             complement(1354..1800)
                     /locus_tag="PLB01_16075"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQQ78872.1"
                     /translation="MDRLRGSAETTVDEKGRFKVPAVFREPVEDTFGPDFFLTSISGA
                     DVLIFPMPVWNALEEKLAAMPAIHRAKAKFQERFNTFGQTVRMDAQGRLLVPSLLREV
                     SGLSGDALVLGQTDHLRLVDRTRHLKTLKTATITDEDYDELARHLV"
     gene            1990..3147
                     /locus_tag="PLB01_16080"
     CDS             1990..3147
                     /locus_tag="PLB01_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HQQ78873.1"
                     /translation="MTAPPLSPLPPPAPAPLFVASEGPLAALLDRLDAEPAATVALDT
                     EADSFHHYYEKVCLVQVGLGADAFLVDPLAGVDLAPLFARLSARPLLLHGADYDLRLL
                     FRGYGFRASSLFDTMIAAQLVGEKEIGLAALLAARAGVTLDKAHQRADWSERPLSPSM
                     ASYAAADVVHLPALVASLTADLEAKGRLAWHAEECARLAETAFPSERAADTENDWRIK
                     GSNGVSDRERAFVRAFWEVREARARALDRPPFRVLTNERLLAAAALAAKGERDLGRLF
                     PGPRPLPGPLAQALKGALEAAAAVDPADWPKPRRGERAETDPALERDVDALKKARDAK
                     ALALGLDPGFLGSRAALTAAARVKRAHGQLTPELLATEAGLSRWRAELLAG"
     gene            complement(3155..4834)
                     /locus_tag="PLB01_16085"
     CDS             complement(3155..4834)
                     /locus_tag="PLB01_16085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQQ78874.1"
                     /translation="MTSNRPLFRPLGAAAATLAALVALAPAPPLSASEVVNRILIHVN
                     SRIITQSQFDARMDQSIRENGAPPTPAQREALKKSVMEELVNEALLEDRARELDLITT
                     DQEIEDQIKRLKEQNNVRTDEEFTKSLAASGLTIDRLRDQLRRSQTLQRVVGREVQAK
                     VDLSDDALRLIYEREKEAWRVPEKAHLAEILISKGDDPAAAARRAKEASDLLKGGAKF
                     EAVVKEFSDGGTKAKGGDLGVVAKGELAADIDKAVFSLPVGAVSDPIQTKFGWHLVKV
                     IEKVPVSYKAFADVKADLLKREQDTQFQKKLAEYLEKLKRDAVIRVNPEAQAYFTPPP
                     PPPGFSQTAGPTVFDQAAGVVGVGTGPATKASRDPVVEITPTVGWRWGGTTSESVTSY
                     FEKIGVPNAMSWGLTFEYVITPWGSLEALWSHQNTTLTAQFTAGTVGYDDKLSHLNVD
                     TFQIGGMWMSGDASNKARLYFDLLLGVTVLTPSPGFETITRFSGSVGGGIKYYFADHF
                     GLRAGARWMPVYVNSSASGGGYCDPIYGCYTWYGTNYLNQGDAHLGLIARF"
     gene            complement(4865..>5727)
                     /locus_tag="PLB01_16090"
     CDS             complement(4865..>5727)
                     /locus_tag="PLB01_16090"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQQ78875.1"
                     /translation="GVRVRRPPALAAALLVLDAGCSRNGRPGAPGPSVVATVGDRQVS
                     LEEFFKSARNAAGEDPKNVSPRVLSSLLDQFLDEVLLERAVAAAQPPVTGATPAERRR
                     AYLSRRAGLEKLDDTDLRRDYGAHPDRYRRPALVRVSQMFFPTREAADAALRRLKDGA
                     AWMDVSRGSSAAPNAASGGALGLLARSDLPQEFEKAIWALPAGGTTGILAAPHGFHVF
                     RVEERLDARDITFEEARPGLRISLAEELSAKAVEDAIAEARKAFPVTVLEDHLPFPYV
                     GTNPKAAAAR"
BASE COUNT          788 a         2037 c         2058 g          844 t
ORIGIN      
        1 cggccgcgcg ggcggaggcg ccggagcgag cgccgcgtcc ttcacgagca cgacctggtc
       61 gagcgtcggg ggcacgaggc cgagcgtccg tcgcgagacg tcctcgaccc gcgagagcgc
      121 ggccgcctgg gcgcgatcca tctcgagctt gcgcttcgtc tcgagaagcg cctcccgtct
      181 cttggccacg gtggcgatcc ggtagccgag ctcgaccgtc ttgacgttcg cccagatcgc
      241 gacgaacagc acgatgaccg gcggcacggc ggcgagcgcg aatccgaaga gctcgcgcga
      301 ccggtggcgg tcgcgctccc ggaccaggta gacgttctcg acagggcggc ggaccgcgta
      361 ggccatggga aacccctaac gcggcggcgt cagcgggccg gtctcctcgg cgacgcgctc
      421 gaggacgcgg agacgcgccg agcgggcgcg cgggttctcg ccgagctcgg cgagcgtggg
      481 gcggatggcc ttcttcgtga cgagcgccat cacgcggcgg cgcgagcaga cacacacggg
      541 gaacgacggc gggcacgtgc agccggccgt gtggcgctgg aacgcgcgct tgacgatgcg
      601 gtcctcgagc gagtggtacg agaggacgga gagccgcgcg ccgagcgcga gtcgcgcgat
      661 ggcgtcgtcg aggaagcgct cgagctcgac gagctcccgg ttcgtggcga tccggagggc
      721 ctggaagacg cgcgtcgcgg gatccttgtg cttctcacgg cgggggccga gcgcccggag
      781 gacgaggcgc cgcatctcgc cggtggtccg gatgggagcc gtctcccggg cttccacgac
      841 ccgtcgcgcg atcggtccgg ccatgcgttc ttccccccat tcgcggaaaa tccgcgtcag
      901 ttcgtcccgt gaggcggtcg cgacgaggtc cgcggccgtc gggccttccg gtcccatccg
      961 catgtcgagc ggcccgtcct tcatgaacga gaacccccgt tcggcccggt cgatctgcat
     1021 cgaagaaacg ccgaggtccg cgaggacgcc ggccacgcgg ccgattccca gcgcgtcgag
     1081 atgcgcggcg acgtcctcgt ggcgccccag gaccgtgacg aggcggtccc cgaagcgcgc
     1141 gaggcgctcg cgcgcgagga cgagcgcctc gggatcgcgg tcgatcccca cgacccgaac
     1201 gaggggcgac gcctcgagga gggcctccgc gtgtccgccc aggccgagcg tcgcgtcgac
     1261 gaacacgccg ccgccctccg gccggagagc gtcgacgacc tcacggagga ggacggggac
     1321 gtgacccgcg cgttctcctt ccatgtcgga ccgtcagacg agatggcgcg cgagttcgtc
     1381 gtagtcctca tccgtgatcg tggccgtctt gagcgtcttg agatggcggg tgcggtcgac
     1441 gaggcggagg tggtccgtct gccccaggac gagcgcgtcg cccgagaggc ccgagacctc
     1501 gcgcaggagc gagggcacga gaaggcggcc ctgggcgtcc atccgcaccg tctggccgaa
     1561 cgtgttgaag cgttcctgga atttcgcctt cgcgcggtgg atcgccggca tcgcggcgag
     1621 cttctcctcg agcgcgttcc agacgggcat cgggaagatc aggacgtcgg cgcccgagat
     1681 cgacgtcagg aagaagtccg ggccgaacgt atcctcgacg ggctcgcgga agacggccgg
     1741 gaccttgaag cggcccttct cgtccacggt cgtctcggcg ctgccccgga ggcggtccat
     1801 aatccctctc gcgaaaagcg acggcggagg cgatggcagg ccccaggggc ccaccattca
     1861 ccaccactga ccactttttt ccaccagcga gtatccggcg gctccgccgg gggcgtcaag
     1921 agaaatccct tctcacgagc gggttgcggc ccgggggctc ggcgcggcgg ggtggtaccc
     1981 tcgccgcccg tgacggcgcc gcccctttct cccctcccgc ctcccgctcc cgcgccgctc
     2041 ttcgtcgcct ccgaaggtcc gctcgcggcg ctcctcgacc ggctcgacgc ggagccggcg
     2101 gcgaccgtcg cactcgacac ggaggccgat tccttccacc actactacga aaaggtgtgt
     2161 ctcgttcagg tcggcctcgg ggcggacgcc ttcctcgtgg acccgctcgc cggcgtcgat
     2221 ctcgcgcccc tcttcgcgcg gctctcggca cggccgctcc tcctgcacgg ggccgactac
     2281 gatctccgac tcctgttccg cggctacggc ttccgggcgt cgtcgctctt cgacacgatg
     2341 atcgcggcgc agctcgtcgg cgaaaaagag atcggcctcg cagcgctcct cgcggcacgc
     2401 gccggcgtca cgctcgacaa ggcgcaccag cgggcggact ggagcgagcg gccgctctcg
     2461 ccgtcgatgg cctcgtacgc ggcggccgac gtcgtccacc tgcccgcgct cgtcgcgtcg
     2521 ctcacggccg acctcgaggc gaagggccgc ctcgcgtggc acgcggagga gtgcgcacgc
     2581 ctcgcggaaa ccgcgttccc ctccgagcgc gccgccgaca cggaaaatga ctggcgcatc
     2641 aagggctcga acggcgtgtc cgacagggag cgcgcgttcg tgcgggcgtt ctgggaggtc
     2701 cgcgaggctc gcgcccgcgc cctcgaccgg ccgcccttcc gcgtcctcac gaacgaacgg
     2761 ctcctcgcgg ccgccgcgct ggcggccaaa ggcgagcgcg acctcggacg gctctttccc
     2821 gggccgcggc ctctcccggg tccgctcgca caggcgctga agggcgccct cgaggccgcc
     2881 gcggccgtcg atcccgccga ctggccgaaa ccgcggcgcg gagagcgggc cgaaacggac
     2941 cccgcgctcg agcgggacgt ggacgcgctc aagaaggcgc gcgacgcgaa ggctctcgcg
     3001 ctcggcctcg accccggctt cctcgggtcg cgcgcggcgc tcacggcggc ggcgcgggtc
     3061 aagcgcgcgc acgggcagct cacgcccgaa ctcctcgcga ccgaagccgg cctctcgcgc
     3121 tggcgcgcgg agctcctcgc gggctgaccc ctcctcagaa cctcgcgatg aggccgaggt
     3181 gggcgtcgcc ctggttcaga tagttcgtcc cgtaccaggt gtagcagccg tagatcggat
     3241 cacagtagcc gccgccgctt gcggacgagt tgacgtagac gggcatccag cgcgcaccgg
     3301 cgcgcaggcc gaaatggtcg gcgaagtagt acttgatccc gccgccgacg cttcccgaga
     3361 agcgtgtgat cgtctcgaac ccgggcgagg gcgtcaggac cgtgacgccg aggaggaggt
     3421 cgaagtagag gcgggccttg ttgctcgcgt cgcccgacat ccacatgccg ccgatctgga
     3481 acgtgtcgac gttcaggtgg gagagcttgt cgtcgtaccc gacggtgccc gcggtgaact
     3541 gcgcggtgag cgtcgtgttc tggtggctcc agagcgcctc gagggagccc cacggcgtga
     3601 tcacgtactc gaacgtcagg ccccagctca tggcgttcgg cacgccgatc ttctcgaagt
     3661 aggacgtcac ggactccgac gtcgtgccgc cccagcgcca gccgaccgtc ggcgtgatct
     3721 cgacgaccgg gtcgcgcgac gccttcgtgg ccgggcccgt gccgaccccg accacgccgg
     3781 cggcctggtc gaagacggtg gggccggcgg tctgcgagaa gcccggaggg ggcggcggcg
     3841 gcgtgaaata ggcctgcgcc tcggggttca cgcggatgac ggcgtcccgc ttgagcttct
     3901 cgaggtattc ggcgagcttc ttctggaact gggtgtcctg ctcacgtttc aggaggtcgg
     3961 ccttcacgtc ggcgaacgcc ttgtacgaca ccggcacctt ctcgatgacc ttcacgaggt
     4021 gccagccgaa cttcgtctgg atcgggtcgg acacggcccc gaccgggagc gagaagacgg
     4081 ccttgtcgat gtcggccgcg agctcgccct tggcgacgac ccccaggtcg ccgcccttgg
     4141 ccttcgtgcc gccgtcggag aattccttca cgacggcctc gaacttcgcg ccgcccttga
     4201 gcaggtcgga cgcttccttg gcccggcgcg ccgcggccgc ggggtcgtcg cctttcgaga
     4261 tcaggatctc ggcgaggtgc gccttctcgg gcacccgcca cgcttccttt tcgcgttcgt
     4321 agatgagccg gagcgcgtcg tccgagaggt cgactttcgc ctgcacctcg cgcccgacga
     4381 cgcgctggag cgtctgcgag cggcggagct ggtcgcgcag gcggtcgatc gtcaggcccg
     4441 acgccgcgag gctcttcgtg aactcctcgt cggtcctgac gttgttctgt tccttgagcc
     4501 gcttgatctg atcctcgatc tcctggtccg tcgtgatgag gtcgagctcg cgggcccggt
     4561 cctcgaggag cgcctcgttg acgagctcct ccatgacgct cttcttgaga gcctcgcgct
     4621 gcgccggggt cggcggcgcg ccgttctcgc ggatggactg gtccatccgg gcgtcgaact
     4681 ggctctgcgt gatgatccgc gagttcacgt ggatgaggat ccggttcacg acctcgctgg
     4741 cggagagcgg gggcgccggg gcgagggcga cgagcgcagc cagagtggcg gccgccgcgc
     4801 cgagcggtct gaaaaggggc cgatttgacg tcacgaggcc ggagtctagc gaaatccggg
     4861 ccgcttaccg ggctgcggcc gctttcgggt tcgtcccgac gtacgggaac ggcaggtgat
     4921 cctcgaggac cgtcaccgga aacgccttcc gcgcctcggc gatcgcgtcc tcgacggcct
     4981 tcgcgctgag ctcctcggcg agcgagatgc gcaggccggg acgcgcctcc tcgaacgtga
     5041 tgtcccgcgc gtcgaggcgc tcctccacgc ggaagacgtg aaagccgtgc ggcgccgcga
     5101 ggatgccggt cgtgccgccc gcgggcagcg cccagatcgc cttctcgaac tcctgcggga
     5161 ggtcggaccg ggcgaggagc ccgagggcgc cgccggaggc cgcgttcggc gccgcgctcg
     5221 agccgcgtga cacgtccatc cacgccgcgc cgtccttgag gcgccggagc gccgcgtccg
     5281 cggcctctcg cgtcgggaag aacatctgtg agacccgcac gagcgcgggc cgccggtaac
     5341 ggtccgggtg cgcgccgtag tccctgcgga ggtccgtgtc gtcgagcttc tcgagcccgg
     5401 cccgccggga gaggtacgcg cggcgccgct cggccggcgt tgcgcccgtg acgggcggct
     5461 gcgcggcggc gacggcccgc tcaaggagaa cctcatcgag aaactggtcg aggaggctcg
     5521 agaggacgcg cggcgagacg ttcttcgggt cctcgccggc cgcgttccgc gccgacttga
     5581 agaattcctc cagcgagacc tgccggtccc ccaccgtcgc gacgacggac ggcccgggcg
     5641 ctcccggccg gccgttccgc gagcagcccg cgtcgagtac gaggagggcg gccgcgagcg
     5701 ccggcggccg cctcacgcga acgcctc
//
LOCUS       DAOMFY010000256         5715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4805_length_5715_cov_5.724028, whole genome shotgun sequence.
ACCESSION   DAOMFY010000256 DAOMFY010000000
VERSION     DAOMFY010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4805_length_5715_cov_5.724028"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PLB01_16095"
     CDS             <1..1086
                     /locus_tag="PLB01_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQQ78876.1"
                     /translation="LVGLIDPPRANVRGAIEELRSAGIRTVMVTGDQKGTAMAVARTL
                     AIAGPEDLCLDSAELALYLQEHRWEDLRRTAVFARVTPEDKLTIVHALREAGRVVAMT
                     GDGINDAPALKAADIGIAIGLGSADVARAASDLVVTSGDYATIPTAVAEGRQIYANIR
                     RAVHFLLLCSLSTIGVMLVAVVTNLPLPMSPLQILWLNLVVHIFPAVALVLVPGETDV
                     MSRPPRDPKEPLLTWGATGRIALKSAVVAAAVLWSYTAGGGPGDAPRGQTLVMATLAL
                     TLLVATFPGLSETGPFWRMGRSMTPPFWLALAGGLGLQALALHWPPLASVLITEPLSA
                     PDWLRVLGMAAVALAAVEALKRLATPR"
     gene            complement(1090..2262)
                     /locus_tag="PLB01_16100"
     CDS             complement(1090..2262)
                     /locus_tag="PLB01_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQQ78877.1"
                     /translation="MKKKRTALVVAAVVLLGAVAVALRLRPEAVPVDLGRCDQGPLVV
                     TVDEEGKTRVVDRFVVSAPVAGKLARLEVHEGDALARGDVVARLDPLPLDARTRAELS
                     ARHESTRAAERAARARELQAAAAAEQAKRNRDRIEKLAKKGVMAPEEREQAELAETVR
                     LRELEAARLVVRAAGFDAAAAQAALLAAESGRGEALTVVRCPAAGRVLKVVQESERAV
                     VAGTPLLEVGDPSKLEIVLDVLSRDAVRVRPGARMWLEEWGGPEALPATVRLVEPSAF
                     TKLSALGVEEQRVRVVGDLASAPAGLGDGFRVEARIVVDEAKDVVRVPATSLFRKGDA
                     WAVFTVAGGRARLKKVETGLANRTHVEIRGGLAAGDSVVLHPSDRVADGVRLRERK"
     gene            complement(2269..4632)
                     /locus_tag="PLB01_16105"
     CDS             complement(2269..4632)
                     /locus_tag="PLB01_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ78878.1"
                     /translation="MKTLDRMLVRDLWHLRGPLAAITVVVACGVATVVTTFTAYESLV
                     LSQEAYYEQYRFADVFASLKRAPESVARTLERIPGVAAVETRIVFEVTADVAGLAEPA
                     TLRLVSIPDRAPPRLNALHVRSGRLPEPGRRNEVVASDAFASANGLKPGGTVGAILNG
                     RWETLLIVGVGLSPEYVYEVRAGDILPDNRRFGVVWMGRDAMAPAFSMDGAFNDVVLG
                     LDAKAREGDVLAEVDRVLAPWGGLGAVAARDQVSNRFLSDEIAENQATGTVIPFVFLG
                     VSAFLLNLVLSRLVSTQREQIGVLKAFGYSNAVLGGHYLKLALAAVLAGSALGVAAGV
                     RLGVAVNELYGQYFRFPVMRYEANVTVIGVAVGLTLAAAVVGSLGAVRRAVKLPPAEA
                     MRPEAPPRFRPGPLDRLGLARRLATPGRIILRNLERRPGRAVLSAFGIALAFAILVLG
                     RFLGDVVQWLVDVQFREIQREDMTIVFQQPRPRETRFEVERLPGVRRAETFRAVPARL
                     RFEHRSRRVAILGLDRGAELRRLLDRDLRPVPLPPDGFLLGAGLADILEARPGDVLTA
                     EVLEGARPTRRVVLAGVVEEPVGLSVYMERGALDRLLGDEGAVSGAYLAVEPSAVDAL
                     YARLKRTPAVAGASFRTVALASFEETFARSFGVMTGIIVFFACAIAFAVVFNSARISL
                     AERARELASLRVLGFTTREVSRMLLGEQAVLTCAAIPLGIATGTWVCRAILPLYQRET
                     FRLPLVLTPATYAFAALVVAAAAALSALAVSGRIRRLDLIEVLKSRE"
     gene            complement(4634..5395)
                     /locus_tag="PLB01_16110"
     CDS             complement(4634..5395)
                     /locus_tag="PLB01_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ78879.1"
                     /translation="MRRHYRRCFRPGLTLIQVVEAPVLRARGLTKVYRTGEVEVHALR
                     ATNLEIRRGEFLVLLGPSGGGKSTLLNILGGLDVPTSGSVLFFDHDLTAADDRERTRF
                     RREHVGFVFQFYNLIPSLTARENVALVTEIASNPLDPAECLRLVGLGDRLDHFPAQLS
                     GGEQQRVAIARAIAKRPDVLLCDEPTGALDAATGKLVLEALVEVNRTLATTTAVITHN
                     AAVAGIADRVLRMHSGRIASETRNEKKVAPADLEW"
     gene            5433..>5715
                     /locus_tag="PLB01_16115"
     CDS             5433..>5715
                     /locus_tag="PLB01_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQQ78880.1"
                     /translation="MATALTLLVPVDFSPASLKAVREAGRLADAMVASVTLLHVRPIS
                     DVMAAIAEGREDLIKGNARALAAALKRHYADRLAALATRSGAVEARLATG"
BASE COUNT          839 a         2107 c         2036 g          733 t
ORIGIN      
        1 ctcgtgggac tcatcgaccc gccgcgggcg aacgtccgcg gcgcgatcga agaactccgc
       61 agcgccggca tccgcaccgt catggtgacc ggcgaccaga agggaaccgc gatggccgtc
      121 gcgagaacgc tcgcgatcgc cggtccggaa gacctctgtc tcgactcggc cgagctcgcc
      181 ctgtatctcc aggagcaccg ctgggaggac ctgcgccgga ccgccgtctt tgcgcgcgtc
      241 acgccggagg acaagctcac aatcgtccac gcccttcgag aggccggacg cgtggtcgcg
      301 atgacgggag acggcatcaa cgacgcgccc gcgctaaagg cggcggacat cggaatcgcg
      361 atcgggctcg gctcggcgga cgtcgcgcgc gcggcctccg atctcgtcgt cacgagcggc
      421 gactacgcga cgattccgac cgcggtagcg gagggccgcc agatctacgc gaacatccgg
      481 cgcgccgtcc acttcctgct gctctgcagc ctgtcgacga tcggcgtcat gctcgtggcg
      541 gtcgtcacga acctgccgct cccgatgagc ccgctccaga tcctctggct caacctcgtc
      601 gtgcatatct ttcccgcggt cgcgctcgtc ctcgtgccgg gcgagaccga cgtgatgagc
      661 cgccccccac gcgacccgaa ggagccgctc ctcacgtggg gagcgacggg ccggatcgcc
      721 ctcaagagcg ccgtcgtcgc cgcggccgtc ctctggagct acacggcggg cggcgggccc
      781 ggcgacgccc cgcgcggcca gaccctcgtg atggcgacgc tcgcgctgac gctcctcgtc
      841 gcgaccttcc cgggcctctc ggagacgggc ccgttctggc gcatgggacg aagcatgacc
      901 ccgcccttct ggctcgcgct cgccggcggc ctcggtctgc aggccctcgc gctgcactgg
      961 ccgcctctcg cctcggtgct catcacggag ccgctctcgg caccggactg gctgcgcgtc
     1021 cttgggatgg cggcggtcgc cctcgcggcc gtcgaggccc tcaagcggct cgccacgcct
     1081 cgctgaggct cacttccgct cccgcaggcg cacgccgtcc gccacgcggt cgctcggatg
     1141 gaggacgacc gaatctccgg cggcgaggcc gccgcggatc tcgacgtgcg tccgattcgc
     1201 gaggcccgtc tcgaccttct tcagacgcgc ccgccccccg gcgaccgtga acaccgccca
     1261 cgcgtcgccc ttgcggaaga gagaggtcgc cgggacgcgc acgacgtcct tcgcctcgtc
     1321 gacgacgatg cgcgcctcga cgcggaaccc gtcgccgagc cccgccggcg cgctcgcgag
     1381 gtcgcccacg acgcggacgc gctgctcctc gacgccgagc gccgagagct tcgtgaacgc
     1441 cgacggctcg acgagacgca cggtcgccgg cagcgcctcg ggaccgcccc attcctcgag
     1501 ccacatgcgc gccccgggcc ggacacggac cgcgtcgcgg gagaggacgt ccagcacgat
     1561 ctcgagtttc gagggatccc cgacctcgag cagcggcgtc cccgcgacga cggcgcgctc
     1621 gctctcctgg accaccttga gcacgcgccc cgccgcgggg cagcggacga cggtcagggc
     1681 ctcgccgcgg cccgactcgg ccgcgagaag agcggcctgc gcggccgccg cgtcgaatcc
     1741 cgcggcgcgg acgacgagac gcgcggcttc cagctccctc aggcgcacgg tctcggcgag
     1801 ctcggcctgc tcgcgctctt cgggcgccat gacgcccttc ttcgcgagct tctcgatccg
     1861 gtcgcggttc cgcttcgcct gttcggcggc ggcggccgcc tggagctccc gcgcccgggc
     1921 ggcccgttcg gcggcgcgcg tggactcgtg gcgcgcggag agctctgccc gcgtccgcgc
     1981 gtcgagggga agcggatcga gacgcgcgac cacgtcgccg cgggcgagcg cgtccccctc
     2041 gtggacctcg agccgtgcga gcttcccggc caccggcgcg ctcacgacga agcggtccac
     2101 gacgcgggtc tttccctctt cgtcgaccgt gacgacaaga ggtccctggt cgcaccgccc
     2161 gaggtcgacg ggcaccgcct cgggccggag gcgaagcgcg acggccacgg ccccgaggag
     2221 gaccacggca gcgacgacga gggcggtgcg tttcttcttc atgggcggtc actccctcga
     2281 tttcaggact tcgatcaggt cgagccgccg gattcgcccc gagacggcga gcgcggacag
     2341 ggccgcggcg gccgcgacga cgagcgcggc gaacgcgtac gtcgcgggcg tcaggacgag
     2401 cggaaggcgg aacgtctcac gctggtagag cggaaggatg gcccggcaga cccacgtccc
     2461 cgtcgcgatg ccgaggggaa tcgcggcgca cgtgaggacg gcctgctccc cgagcagcat
     2521 tcgcgagacc tcccgggtcg tgaagccgag gacgcgcagg ctcgcgagct cgcgcgcgcg
     2581 ctccgcgagg gagatgcgcg cggagttgaa gacgaccgcg aacgcgatcg cgcaggcgaa
     2641 gaagacgatg atccccgtca tgaccccgaa actcctcgcg aacgtctcct cgaaggacgc
     2701 gagcgccacg gtgcggaacg acgcgcccgc gacggccggc gtgcgcttga gccgggcata
     2761 gagggcgtcc acggcggaag gctcgacggc gaggtacgcc cccgagacag ccccctcgtc
     2821 gccgaggagg cggtccaggg cgccgcgctc catgtacacg gacaatccga ccggctcctc
     2881 gacgactccc gcgagaacga cgcgccgcgt cgggcgcgcg ccctcgagga cttccgcggt
     2941 gagcacgtct cccgggcgcg cttccagaat gtcggccagc ccggccccga ggaggaagcc
     3001 gtcgggcggc agcggcacgg gtctcaggtc gcggtccaga aggcggcgca gctcggcgcc
     3061 gcggtcgagc ccgagaatcg cgacgcgccg gctgcgatgc tcgaacctca ggcgcgccgg
     3121 gacggcccgg aacgtctcgg cgcgccggac gcccggcagg cgttcgacct cgaagcgggt
     3181 ctcgcggggg cgcggctgct ggaagacgat cgtcatgtcc tcccgctgga tctcgcggaa
     3241 ctgaacgtcg acgagccact gcacgacatc ccccaggaac cgaccgagga cgaggatcgc
     3301 gaacgcgagc gcgatgccga aggccgagag gaccgcgcgg cccgggcgtc gttcgaggtt
     3361 gcggaggatg atccggcccg gcgtcgcgag gcggcgcgcg aggccgagcc ggtcgagcgg
     3421 cccgggtctg aacctcggcg gcgcctcggg ccgcatcgcc tcggccggag gcagcttcac
     3481 ggcgcgcctc acggcgccga gcgaccccac gacggccgcc gcgagcgtga gccccacggc
     3541 cacgccgatg acggtgacat tcgcctcgta gcgcatgacg gggaagcgga agtactgccc
     3601 gtagagctcg ttcacggcga ctccgaggcg caccccggcg gcgacgccga gcgccgagcc
     3661 ggcgagcacg gccgcgagcg ccagcttcag gtaatggccg ccgaggaccg cgttcgagta
     3721 gccgaacgcc ttgagcacgc cgatctgctc gcgctgcgtc gagacgagcc gcgacaggac
     3781 gaggttcagc aggaacgccg aaacgcccag gaaaacgaac ggaatgaccg tgccggtggc
     3841 ctggttctcg gcgatctcgt cggagaggaa acggttcgac acctggtccc gggcggcgac
     3901 ggcgccgagg ccgccccacg gagcgaggac gcgatccacc tcggcgagca cgtcgccttc
     3961 gcgcgcctta gcgtcgagcc cgaggacgac gtcgttgaac gccccgtcca tgctgaacgc
     4021 cggcgccatg gcgtcgcggc ccatccagac gacgccgaag cggcggttgt cgggcaggat
     4081 gtcgccggcc cgcacctcgt agacgtactc gggcgagagg ccgacgccca cgatcagaag
     4141 cgtctcccac cgtccgttca ggatcgcacc gacggttcct ccgggcttca gcccgttcgc
     4201 gctcgcgaag gcgtcgctcg cgacgacctc gttccgccgg ccgggttccg ggaggcgccc
     4261 gctccgcacg tgcagggcgt tcagacgggg cggcgcgcga tccgggatcg acacgaggcg
     4321 cagggtcgcg ggttccgcga gtcccgcgac gtcggccgtc acctcgaaga cgatccgcgt
     4381 ctcgacggcc gcgacgcccg ggatccgctc gagcgtccga gcgaccgact ccggcgcgcg
     4441 cttgagcgag gcgaagacgt ccgcgaatcg gtactgctcg tagtaggcct cctgggagag
     4501 gacgagagac tcgtaggcgg tgaacgtcgt cacgaccgtc gcgacgccgc acgcgaccac
     4561 cacggtgatg gccgcgagcg gcccgcggag gtgccagagg tcccggacga gcatgcggtc
     4621 gagcgtcttc acgtcaccac tccaggtcgg cgggcgcgac cttcttctcg tttcgggtct
     4681 cggaggcgat gcggccgctg tgcatcctga ggacgcggtc cgcgatcccc gcgacggccg
     4741 cgttgtgcgt gatcacggcc gtcgtcgtgg cgagcgtgcg gttcacctcg acgagcgcct
     4801 cgagcacgag ctttccggtc gccgcgtcga gggcgcccgt cggctcgtcg cagagcagga
     4861 cgtccggcct cttcgcgatc gcgcgcgcga tcgcgacgcg ctgctgctcg cccccggaga
     4921 gctgagccgg gaagtggtcc aggcggtccc cgaggccgac gaggcgcagg cactcggcgg
     4981 gatccagcgg gttcgaagcg atctcggtca cgagcgcgac gttctcgcgc gccgtcaggc
     5041 tgggaatcag gttgtagaac tggaacacga agcccacgtg ctcgcggcga aagcgggtcc
     5101 tctcccggtc gtccgcggcg gtgagatcgt ggtcgaagaa gaggaccgat ccgctcgtgg
     5161 ggacgtcgag gccgccgagg atgttcagga gcgtcgactt gccgcctccg gagggcccca
     5221 ggagaacgag gaactccccc cgccggatct cgagattggt ggcacgaagc gcgtgcactt
     5281 cgacctcccc ggtcctgtag accttggtga ggcctcgcgc gcgcaggaca ggcgcctcga
     5341 ccacctgaat caaggtgagg cccgggcgga aacagcgtct gtagtgccga cgcatcaggc
     5401 gcccgaccgc ctcccgcgct agcattgccg gaatggcgac cgccctgacg ctcctcgtcc
     5461 ccgtcgactt ctcccccgcg tccctcaagg ccgtccgcga ggcgggccgg ctcgcggacg
     5521 cgatggtggc gtccgtgacg ctcctgcacg tgcgcccgat ctccgacgtg atggccgcga
     5581 tcgccgaagg gcgcgaggac ctcatcaagg ggaacgcgcg cgcgctcgcg gcggcgctca
     5641 agcgccacta cgccgaccgg ctcgccgccc tcgcgacgcg ctccggcgcc gtcgaggcgc
     5701 gcctcgcgac gggcc
//
LOCUS       DAOMFY010000257         5700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4827_length_5700_cov_8.990611, whole genome shotgun sequence.
ACCESSION   DAOMFY010000257 DAOMFY010000000
VERSION     DAOMFY010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4827_length_5700_cov_8.990611"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLB01_16120"
     CDS             <1..302
                     /locus_tag="PLB01_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78881.1"
                     /translation="KYHEVTPCAGIADAAEKHERFHVTTCKPGQSRSVAQIAQNEVDA
                     YKVELTELENLRKALEKVCEKKTCTVKHEGGDVKKRLEEELELLRQVLVKKGVKK"
     gene            299..1291
                     /locus_tag="PLB01_16125"
     CDS             299..1291
                     /locus_tag="PLB01_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78882.1"
                     /translation="MSAPRAIALLVISASLPVLASQRAVWISAKPGNVATSCGDVNSP
                     ACTAALEALARKAADDLTLLANSGEPGNRPLAREAAKMPFASLRASAASALGRFSPGP
                     EDTPLLSSLLNDPVPRVRRSARSALEFSSDPSARPLAERARLYESDGLRPQAAPTAEQ
                     LKTPLYAGAQYLYYASDRSRGQSEFATGDAVDKVAAFYAGKFGNAMTLEQFQAAAKSG
                     KNAMPDMGSQAFQDQMQAAMDAQKAYEAAIKAGKSQQDASMAMVNAMQAKAPADTSRI
                     RSALERKEIYGSPKLFVVEKGMMPGAPNRLVAVYKDLLLGKTGIAVFTAPLPEE"
     gene            complement(1298..2200)
                     /locus_tag="PLB01_16130"
     CDS             complement(1298..2200)
                     /locus_tag="PLB01_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78883.1"
                     /translation="MHRTRRALALAATLAACGATAQQVRPLTERYAALGTMIVTTSDH
                     AMFPHEKRAKGHTYEGKAYPADVHYSDKTVAIFVPKGFRPGETTDLVFYFHGWRNNVD
                     DTLRQFKPAEQLAASGVNAVLVLPEGPKNAPDSFGGRLEETGVFAGLVADVLGTLKAR
                     GLVATTKPGAIVLAGHSGAYRVMAFILTRGGLTANVKEVYLFDALYGQIEKFAHWIDR
                     FPGKLIDIHSADGGTRAQSLDLMDDLRAWDIPFVSVPESAVTPDLLKRNRLVFVDSPL
                     AHDDVVAKNGQLRAYLEASSLPRR"
     gene            complement(2211..5588)
                     /locus_tag="PLB01_16135"
     CDS             complement(2211..5588)
                     /locus_tag="PLB01_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQQ78884.1"
                     /translation="MRVRLATAGLSLFLSSALSAAPAPAGPSKMLTDPALSADRIAFV
                     YGNDVWTCRLDGGGVSRVTSGPGAKLRPAFSPDGSLLAFSAELDGNLDVYVVPAAGGV
                     PKRLTWHPGRDIVQGFTPDGKSVLFTSPRAASNNRHTQLYTVPVEGGLETALPIPYAN
                     RAAWSPDGKRIAYNPNAPAHLQWKRYRGGTVSTIWLYDVATHAIQRVPQPEGRSNDAD
                     PMWVNGPGGATLALRSDRDGEFNLYALTQTADPNNPSKKSFSLSRLTSYTDFPVLGAS
                     SAAGRILFEQAGTLHLLELSSNKTTDLSITVPGDLLETRPRWAKGAKWIRGAALSPSG
                     ARAAFDFRGEIVTVPAEKGDPRNLTNTVSAHESAPAWSPDGKSIAYVSDESGENEIVV
                     RAQDGKGAPKRLKVPGAGFYSDLVFSPDGKKLVLTDNSLTLWVVDVAGGSSKKIASER
                     LYAPVRYKMLRGSWSPDSRWVAYTLSGPTYIRAAYVYSVDEDKSFALTDGLSDVSDPV
                     FDRGGKLLYFLASTDSGPQRNWFSLQNADARVTNAIYVATLKKGTPSPLAKESDEEKG
                     DARDAKAEGKDKEEDSLKGKEGGRPADSRDGEAAGKGEEKGAKARKPEPVVIDHDGLG
                     SRIVDLPVHPAEISNLQAGSAGQLFFERRADGKTSVQRFDFKDRKTETVVPEADDYVV
                     SFDGKKILSRLKDAWTIGASSGKKPDPKEGGDGRLKLDAIQVRVDPRAEWPEIFDEAW
                     RVNRDYFYDPKMHGRDWAAMKAKYAPFLPYVSSRGDLNRLIQWMCSELAVGHHRTTPG
                     ESFAEATPVPGGLLGADYEVVSGRYRFKKVFGGLNWNPELRAPLSEPGVEVKAGEYLL
                     AVNGRDLAPPENLYARFEATAGKIVEITVGPSPDGKGSRTVNVVPVAEENALRNRDWV
                     ESNLRKVDAATGGRVAYVYVPNTAGLGHTYFKRYFYPQAWKDAVIVDERFNGGGSVAD
                     YYIEALLRQPIAWWTFRYGEDMKTPSASIQGPKVMLIDETAGSGGDLLPWMFRKFKVG
                     TIVGQRTWGGLVGILGFPTLMDGAVVTAPNLAFWTPEEGFGVENVGVPPDVEVEQTPA
                     DVIAGRDPQLEKAIAIVMDELKKNPPVKPKRPAMPVR"
BASE COUNT          856 a         1867 c         2071 g          906 t
ORIGIN      
        1 agaagtacca cgaggtcacg ccctgcgcgg gaatcgcgga cgccgccgag aagcatgagc
       61 ggtttcacgt gacgacgtgc aagccgggac agagccgctc ggtcgcccag atcgcgcaga
      121 acgaggtcga cgcgtacaag gtcgagctca cggagctcga gaatctccgg aaggccctcg
      181 agaaggtctg cgagaagaag acgtgcacgg tgaagcacga gggaggggac gtcaagaaac
      241 ggctcgagga ggagctcgag ctcctgagac aggtgctcgt gaagaagggg gtgaaaaagt
      301 gagcgccccc cgcgcgatcg cccttctcgt tatttcggcg tcgcttcccg ttctggcctc
      361 gcagcgcgcg gtctggatct ccgcgaagcc cggcaacgtc gcgacctcgt gcggggacgt
      421 gaattcgccc gcctgcacgg cggcgctcga ggcgctcgcg cggaaggcgg ccgacgacct
      481 cacgctcctc gcgaactcgg gagagcccgg aaaccggccc ctcgcgcgcg aggccgcgaa
      541 gatgccgttc gcctcgctgc gcgcctcggc ggcctccgcc ctcgggcgct tctcgccggg
      601 gcccgaggac acccctctgc tctcgtccct cctgaacgat ccggtgcccc gcgtgaggcg
      661 ctccgcccgg agcgcgctcg agttcagctc cgatccgtcc gcgcgcccgc tcgcggagcg
      721 tgcgcgcctc tacgagagtg acggcctgcg cccacaggcg gccccgacgg ccgagcagct
      781 caagacgccc ctctatgccg gcgcgcagta cctctactac gcgagcgaca ggtcccgcgg
      841 gcagagcgag ttcgcgacgg gcgacgcggt cgacaaggtg gccgcgttct acgcggggaa
      901 gttcggaaac gcgatgacgc tcgagcagtt ccaggcggca gcgaagagcg gcaagaacgc
      961 gatgccggac atgggctcgc aggcgtttca ggaccagatg caggccgcga tggacgccca
     1021 gaaggcttac gaggcggcga tcaaggcagg gaagtcgcag caggacgcgt cgatggccat
     1081 ggtgaacgcg atgcaggcga aggcgcccgc ggacacgtcg aggataagga gcgcgctcga
     1141 gaggaaggag atctacggct cgccgaagct cttcgtcgtc gagaagggga tgatgccggg
     1201 cgccccgaac cggctcgtcg ccgtgtacaa ggaccttctc ctcggcaaga ccggcatcgc
     1261 ggtcttcacg gcgccgctgc cggaggagtg actccgctca gcggcggggg agggagctgg
     1321 cttcgaggta cgcgcggagc tgcccgttct tcgcgacgac gtcatcgtgc gcgagcgggg
     1381 agtcgacgaa gacgagccgg ttcctcttca ggaggtccgg cgtgacggcg ctctccggga
     1441 ccgagacgaa cgggatgtcc cacgcgcgaa ggtcgtccat gaggtcgagc gactgggcgc
     1501 gggtgccccc gtccgccgag tggatgtcga tcagcttgcc cgggaagcgg tcgatccagt
     1561 gcgcgaattt ctcgatctgg ccgtagagcg cgtcgaagag gtagacctcc ttgacgttcg
     1621 cggtgagccc gccccgcgtg aggatgaacg ccatcacgcg gtacgcgccg ctgtgtcccg
     1681 cgaggacgat cgccccgggc ttcgtcgtcg cgaccagacc gcgcgccttc agcgttccga
     1741 gcacgtcggc gacgaggccc gcgaagactc ccgtctcctc gagcctgccg ccgaaggagt
     1801 cgggtgcgtt cttcggccct tcggggagaa cgagaacggc gttgacgccg ctcgcggcga
     1861 gctgctcggc gggcttgaac tgcctgagcg tgtcgtcgac gttgttgcgc cagccgtgga
     1921 agtagaagac gaggtcggtg gtctctccgg ggcggaaacc cttcggcacg aagatcgcga
     1981 ccgttttgtc gctgtagtgg acgtcggcgg gatacgcctt cccctcgtac gtgtgcccct
     2041 tcgcgcgctt ctcgtgcggg aacatcgcgt ggtccgacgt cgtcacgatc atcgtgccga
     2101 gggcggcgta gcgctctgtc agcggccgca cctgctgcgc agttgcgccg cacgcggcga
     2161 gcgtcgcggc gagagccagg gctcggcggg tccggtgcac ggcggtgggc ctacctcacc
     2221 ggcatcgctg gccgtttcgg cttgaccggc gggttcttct tcagctcgtc catcacgatc
     2281 gcgatcgcct tctcgagctg cgggtcgcgg ccggcgatga cgtcggcggg agtctgctcg
     2341 acctcgacgt ccggcgggac gccgacgttc tcgacgccga agccctcctc cggcgtccag
     2401 aacgcgaggt tcggggccgt gacgacggcg ccgtccatga gcgtcgggaa gccgaggatg
     2461 ccgacgaggc cgccccacgt gcgctggccg acgatcgtcc cgaccttgaa cttccggaac
     2521 atccacggca ggagatcgcc gccggagccg gccgtctcgt cgatgagcat gaccttcggg
     2581 ccctggatcg acgcgctcgg cgtcttcatg tcctcgccgt accggaacgt ccaccacgcg
     2641 atcggctgcc tgagcagcgc ctcgatgtag tagtccgcga cgctcccgcc gccgttgaag
     2701 cgctcgtcca cgatgaccgc gtccttccac gcctgcggat agaagtagcg cttgaagtac
     2761 gtgtgcccga ggcccgcggt gttcgggacg tacacgtacg cgacgcggcc ccccgttgcg
     2821 gcgtcgacct tccggaggtt gctctccacc cagtcgcggt tgcggagcgc gttctcctcc
     2881 gccacgggca cgacgttgac ggtgcgcgag cccttgccgt cgggtgacgg gccgaccgtg
     2941 atctcgacga tcttcccggc cgtggcctcg aagcgcgcgt agaggttttc gggaggcgcg
     3001 aggtcccggc cgttcacggc gaggaggtac tcgccggcct tcacctcgac gccgggctcc
     3061 gagagcggcg cgcgcagctc ggggttccag ttcaggccgc cgaacacctt cttgaagcgg
     3121 tagcgcccgc ttacgacctc gtagtccgcg ccgaggaggc cgcccggcac gggcgtggcc
     3181 tcggcgaacg actcgcccgg cgtcgtgcgg tggtggccga cggcgagctc cgaacacatc
     3241 cactggatga ggcggttcag gtcgccgcgc gacgacacgt acggcaggaa cggggcgtac
     3301 ttcgccttca tcgcggccca gtcccggccg tgcatcttcg ggtcgtagaa gtagtcgcgg
     3361 ttgacgcgcc acgcctcgtc gaagatctcg ggccactcgg cgcgcgggtc gacgcgcacc
     3421 tggatcgcgt cgagcttgag cctgccgtcg ccgccttcct tcgggtcggg tttcttgccg
     3481 gaggaggcgc cgatcgtcca ggcgtccttg agccgggaaa ggatcttctt cccgtcgaac
     3541 gagacgacgt agtcgtcggc ctcgggcacg acggtctcgg tcttccggtc cttgaagtcg
     3601 aagcgctgga cggacgtctt cccgtcggcg cggcgctcga aaaagagctg cccggcgctg
     3661 ccggcctgca ggttcgagat ctcggccggg tggacgggga ggtcgacgat gcgcgagccg
     3721 aggccgtcgt ggtcgatcac gacgggctcg ggcttccgag ctttcgcgcc cttctcctcg
     3781 ccctttccgg ccgcctcgcc atccctcgaa tcggcaggcc tcccaccctc tttacccttc
     3841 aaagaatcct cttctttgtc tttcccttct gccttcgcgt cccttgcgtc gcccttctcc
     3901 tcgtcgcttt ccttcgcgag cggcgaaggc gttcccttct tcagcgtcgc cacgtagatc
     3961 gcgttcgtga cgcgcgcgtc cgcgttctgg agcgagaacc agttccgctg cgggccggag
     4021 tccgtcgagg cgaggaagta caggagcttg ccgccgcggt cgaagacggg gtccgagacg
     4081 tccgagaggc cgtccgtgag cgcgaacgac ttgtcctcgt ccacggagta gacgtaggcc
     4141 gcacggatgt acgtggggcc ggacagcgtg tacgcgaccc agcgcgagtc gggcgaccac
     4201 gatccgcgga gcatcttgta gcggacgggc gcgtagaggc gctcggacgc gatcttcttc
     4261 gaagagccgc ccgcgacgtc gacgacccag agcgtgaggg agttgtccgt caggacgagc
     4321 ttctttccgt ccggggaaaa gacgaggtct gagtagaagc cggcgccggg gaccttgagc
     4381 cgtttcggcg cgcccttgcc gtcctgcgcg cgcacgacga tctcgttctc gccgctctcg
     4441 tcggagacgt atgcgatcga cttgccgtcc ggggaccagg ccggcgcgga ttcgtgggcc
     4501 gagacggtgt tcgtgaggtt gcgcgggtcg cccttctcgg cggggacggt cacgatctcg
     4561 ccgcggaagt cgaacgcggc gcgcgcgccg gagggggaga gggcggcgcc gcggatccac
     4621 ttggcgccct tcgcccagcg cgggcgcgtc tcgaggaggt cgccggggac cgtgatcgaa
     4681 aggtcggtcg tcttgttgga cgagagctcg aggagatgga gcgtgccggc ctgctcgaag
     4741 aggattcggc cggccgccga ggacgcgcca agaacgggga agtcggtgta gctggtgagc
     4801 cgggaaagag agaaggattt cttagaagga ttatttgggt cggccgtctg agtgagagcg
     4861 tagaggttga actcgccgtc gcggtcggaa cgcagcgcca gagtcgcgcc acctggacca
     4921 ttcacccaca tggggtcggc gtcgttggat cgcccctcgg gctgcggcac gcgctgaatc
     4981 gcgtgcgtcg cgacgtcgta gagccagatc gtcgagaccg tgcctccgcg gtagcgcttc
     5041 cactggaggt gggccggggc gttcgggttg tacgcgatcc tctttccgtc gggggaccag
     5101 gccgcacggt tcgcgtacgg gatgggcagg gccgtctcca ggccgccctc gacgggcacg
     5161 gtgtagagct gcgtgtgccg gttgttggac gcggctcgcg gcgacgtgaa gaggacggac
     5221 tttccgtccg gcgtgaagcc ctgcacgatg tcgcggcccg ggtgccacgt gaggcgcttc
     5281 gggacgccgc ccgcggccgg gacgacgtag acgtcgaggt tgccgtccag ctcggcgctg
     5341 aacgcgagga gcgagccgtc cgggctgaat gcggggcgca gcttggcgcc cgggccggac
     5401 gtgacgcgcg agacgccgcc gccgtcgagg cggcaggtcc acacgtcgtt gccgtagacg
     5461 aacgcgatcc ggtcggcgga gagcgcgggg tccgtgagca tcttcgacgg gcccgcgggt
     5521 gcgggcgcgg cagagagcgc cgaggacagg aagagcgaca ggccggcggt ggcgagacgg
     5581 acgcgcatgg aaaccccctc gggacgattc agtcgcgggc gggggattct ggcacgccgc
     5641 gctcagcggg cggggaggcc gagggccgcg ggcgccggag cctcggcgcc ggtggcgacg
//
LOCUS       DAOMFY010000258         5696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4834_length_5696_cov_7.409147, whole genome shotgun sequence.
ACCESSION   DAOMFY010000258 DAOMFY010000000
VERSION     DAOMFY010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4834_length_5696_cov_7.409147"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PLB01_16140"
     CDS             complement(<1..1209)
                     /locus_tag="PLB01_16140"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ78885.1"
                     /translation="MSVPPLVLVVDDDAASRKAMALTLQTDGRRVEEFGDADSALERA
                     VEDPSVALVVTDLKMPGKTGLELASELAAARPDVAVLLVTAHGDVETLLSARALGTVD
                     YVAKPLAKDDLRLRAEAALKRARQAGEIRDLRERLDKRFGFEAILGISKPMERLFEKL
                     RVVSPAKTTVLLVGESGTGKELVANALHHNSPRRGRAFVALNCGAIPREIIESELFGH
                     ERGAFTGALVKRTGRIEQAAGGTLFLDEVSEMPPDLQVKFLRVLEEKRVTPVGGNESK
                     DVDFRLVAATNRDLVKEIEAGRFRQDLFYRLSVVTLEIPPLRERKDDIPLLVERFRAL
                     FAEEHGKSVAGATPAALAALVAYEWPGNVRELKNVLESAVLFAAGPRIDVSDLPPAIR
                     AHAPGRPRSGE"
     gene            complement(1206..3248)
                     /gene="ligA"
                     /locus_tag="PLB01_16145"
     CDS             complement(1206..3248)
                     /gene="ligA"
                     /locus_tag="PLB01_16145"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015373821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQQ78886.1"
                     /translation="MTKTPAARAAELRDEVSRHARLYYVEDRPEITDAEYDALLRELI
                     ALEEAHPELVTPDSPTQRVGGPPVSSLPNVKHEIPLLSLENAYSPDELKAWADRVVDR
                     LGRTPEFVCELKIDGLSVALVYEDGRLARAATRGDGTTGEDVTPNVKTIHAVPLDLHA
                     AHAARKRSSKVKIPAVLEVRGEVYMSKKSFAALNEKREAAGEALFANPRNSAAGSLRL
                     LDAKITASRRLSAFLYSIARWDGPGEPQKQTESLFTLEEFSFPVNPHRAVVKDVDGIL
                     AFLEEWKAKRHELPFETDGVVVKVDALSDQKRLGQTAKFPRWAIAYKYPPEEATTVVT
                     DIVVQVGRTGVLTPVAEFTPVLLAGSTVRRATLHNMEDLSRKDVRVGDTVAVEKAGDV
                     IPKVTRVLLERRPKGAKAFSMPARCPACGEPVVQREGEVAVRCVNPGCPAQVAEALRH
                     FVTRRAMDVEGLGDERIEQLREAGLLKDVASLYDLEAAQLAPLERWGDKSAANVIAEI
                     ERSKDAGLARLLFGLGIRQVGEKTAKVLARRFLSMDALAAADEEALTSVPEIGPETAR
                     GILEWFAHPPNRNLLKKLGKAGVRMTEEAGSAGPGGALSGGVFVLTGTLPRRTRDEAA
                     EAIEMAGGKVSSSVSKKTTAVIAGEEAGSKLEKAKALGVPVWSEDDLDRELKGGKK"
     gene            3308..5314
                     /locus_tag="PLB01_16150"
     CDS             3308..5314
                     /locus_tag="PLB01_16150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQQ78887.1"
                     /translation="MIHSRAARLAALAALCAAARLDAGPRVPAVASYTIEASVNDEHV
                     VAGKETVRFTNRTRHTFTDLALHLYLNAWRNDRSTWLVEQARGGGRADRGLAAKDSAR
                     WGWSEIHRIALDDGTDLTSGLTYVSPDDGNADDRTLVSVPLPRPVAPGETLVFRVDWE
                     ARFPRAVARTGWKDDYILGAQWFPKLGVATDAGWNAHQFHAGTEFFADFGDYDVTLSL
                     PAANKGHIGGTGVLKEETELAGGLVRARFAAEDVHDFAFAACPRFVVVRDTFSAKGLP
                     NVDIVLLLQPDHRPLRERYLKATKVGLEDFGTRYLPYPYPVVTVVDPPWGSHTEGMEY
                     PTLFVGGGREIAPKKAHSPESVTIHEYGHQVFYGMLASNEVEEAHLDEGFTTYATTKA
                     LEAAYGNPSLVVRFYGLPVTFAGVTVPYPFGTTDRFHRWQLAARSDAQAVPSYRQLNG
                     DAVRNNAYLRTTLLLRSCERTFGEGVWAKVMKTYATRFAFKHPATADFLGVVREVAGA
                     GAAESVAGIVATAGSVDYAVTAVETHEASGPTGFTGEGPERKFEAAKKGGTGAYESTV
                     VAQRFGEAVWPVDVVFTFADGSTLTRPWSGAESWVRWRLRGPKLVSAEVDPARKCLLD
                     GNTLNNGRRTEPDARASRAWTARFMFWAQNLLEAFSLLGWAAFP"
     gene            5311..>5696
                     /locus_tag="PLB01_16155"
     CDS             5311..>5696
                     /locus_tag="PLB01_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78888.1"
                     /translation="MRRDSVLVLAARGALSALGTWKALLLALVLNLLLAYAFAHPVNA
                     ALRQALDKSPWAARLAAANAPLFEFYGEISRARPDVFGDLSAWDAIATGDEGERSAAR
                     RAPLSGFLSATGDFDHDGISDLAVTNR"
BASE COUNT          771 a         2062 c         2003 g          860 t
ORIGIN      
        1 ctcgcccgag cgcggccggc ccggggcgtg cgcgcggatc gccggcggca ggtccgagac
       61 gtcgatgcgc gggcccgccg cgaagaggac ggcgctctcg aggacgttct tgagctcccg
      121 cacgttgccc ggccactcgt acgcgacgag ggccgcgagg gcggcgggcg tcgcgcccgc
      181 gacggacttg ccgtgctcct cggcgaagag cgcccggaaa cgctcgacga ggagcggaat
      241 gtcgtccttg cgctcgcgca ggggcgggat ctcgagcgtg acgacggaaa ggcggtagaa
      301 gaggtcctgc cggaagcggc cggcctcgat ctccttgacg agatcgcggt tcgtcgccgc
      361 gacgagccgg aagtcgacgt ccttcgactc gttcccgccc accggcgtca cgcgcttctc
      421 ctccaggacg cgcaggaact tcacctgcag gtccggcggc atctcggaca cctcgtcgag
      481 gaagagcgtg cccccggcgg cctgctcgat gcgtcccgtg cgcttcacga gcgcgcccgt
      541 gaacgcgccg cgctcgtggc cgaacagctc ggactcgatg atctcgcgcg ggatcgcgcc
      601 gcagttcagg gcgacgaagg cgcgcccgcg ccgcggcgag ttgtggtgga gcgcgttcgc
      661 gacgagctcc ttgcccgtgc cggactcgcc tacgagaaga accgtcgtct tcgccgggga
      721 gacgacgcgg agcttttcga agaggcgctc catcggcttc gagatcccga ggatcgcctc
      781 gaacccgaag cgcttgtcga ggcgctcgcg caggtcccgg atctcgcccg cctgccgcgc
      841 gcgcttcaag gccgcctcgg cgcggagtcg gaggtcgtcc ttcgcaaggg gcttggcgac
      901 gtagtcgacc gtgccgagcg cgcgcgccga gagcagcgtc tcgacgtcgc cgtgcgccgt
      961 gacgaggagg accgcgacgt ccgggcgcgc cgcggcgagc tcggaggcga gctcgaggcc
     1021 ggtcttgccg ggcatcttga ggtccgtcac gacgagcgcg accgacgggt cctcgacggc
     1081 gcgctcgagg gcggaatccg cgtcgccgaa ctcctcgacg cggcgcccgt cggtctgcag
     1141 cgtgagcgcc atcgccttgc ggctggcggc gtcgtcgtcg accacgagga cgagcggcgg
     1201 cacgctcact tcttaccgcc cttcagctcg cggtcgaggt cgtcctcgga ccacacgggg
     1261 acgccgagcg ccttcgcctt ctccagcttg cttccggcct cttcgccggc gatgaccgcc
     1321 gtcgtcttct tcgacacgct cgagctcact ttcccgcctg ccatttcgat cgcctcggcg
     1381 gcttcgtccc gcgtccgccg gggcagggtc cccgtgagga cgaaaacgcc tccggacagg
     1441 gctccgcccg gccctgcgga cccggcttcc tccgtcatac gaaccccggc cttgccgagt
     1501 ttcttcagga ggttccggtt cggggggtgg gcgaaccact ccaggattcc gcgggcggtc
     1561 tccgggccga tctcgggaac ggacgtgagc gcctcttcgt ccgccgcggc gagcgcgtcc
     1621 atcgagagga agcgccgcgc gaggaccttc gcggtcttct cgcccacctg ccggatgccg
     1681 aggccgaaga ggaggcgcgc gaggcccgcg tccttcgacc gctcgatctc cgcgatcacg
     1741 ttcgcggccg acttgtcgcc ccagcgctcg agcggcgcga gctgcgcggc ctcgaggtcg
     1801 tagagcgacg cgacgtcctt caggaggccc gcctcgcgca gctgctcgat gcgctcgtcg
     1861 ccgagcccct cgacgtccat cgcccggcgg gtcacgaagt ggcggagcgc ctccgcgacc
     1921 tgcgccgggc agcccgggtt gacgcaccgg accgcgacct cgccctcgcg ctggacgacc
     1981 ggctcgccgc acgccgggca gcgcgcgggc atcgagaacg ccttcgcgcc cttcggccgc
     2041 ctctcgagga ggacgcgcgt gaccttcggg atcacgtcgc cggccttctc gacggcgacg
     2101 gtgtcgccga cgcggacgtc cttgcgcgaa aggtcctcca tgttgtggag cgtcgcgcgg
     2161 cgcacggtcg acccggcgag gagcacggga gtgaactccg caaccggcgt caggacgccg
     2221 gtgcggccga cctgcacgac gatgtccgtc acgaccgtcg tcgcctcctc gggcgggtac
     2281 ttgtaggcga tcgcccagcg cgggaacttc gccgtctggc cgaggcgctt ctggtcggag
     2341 agcgcgtcca ccttcacgac gacgccgtcc gtctcgaacg gcagctcgtg gcgcttcgcc
     2401 ttccactcct cgaggaacgc gaggatcccg tccacgtcct tgacgacggc gcgatgggga
     2461 ttgaccggga aagagaattc ttcgagtgtg aagaggcttt cggtctgttt ctgcggttcc
     2521 ccggggccgt cccatcttgc gatggagtag aggaaggcgg agaggcgtcg gctggccgtg
     2581 atcttcgcgt cgagcagccg cagcgagccc gcggccgagt tgcggggatt cgcgaagagc
     2641 gcctcgcccg ccgcctcgcg cttttcgttc aacgccgcga acgacttctt cgacatgtac
     2701 acctcgccgc gcacttcgag cacggcgggg atcttcacct tcgaagaacg ctttcgcgcc
     2761 gcgtgagcgg cgtgcagatc caatggcacg gcatggatcg tcttcacgtt cggggtcacg
     2821 tcctcccccg tcgtgccgtc gccgcgcgtg gccgcgcgcg cgaggcggcc gtcctcgtag
     2881 acgagcgcca cggagaggcc gtcgatcttc agctcgcaga cgaactccgg cgtccgcccg
     2941 aggcggtcca cgacccggtc ggcccaggct ttcagctcgt cgggcgagta ggcgttttcg
     3001 agcgagagga gcgggatctc gtgcttcacg ttcgggaggc tggagacggg tggaccgccg
     3061 acgcgctgcg tcggggaatc cggggtcacg agctcgggat gcgcctcctc cagcgcgatc
     3121 agctcgcgca ggagcgcgtc gtattcggcg tccgtgatct ccgggcggtc ctcgacgtag
     3181 tagagacgcg cgtgcctcga gacctcgtcg cgcagctcgg ccgcgcgcgc cgcgggggtt
     3241 ttcgtcatca ccacggccct gattctaggc ggcgggcccc ccaacggccc tgctagcatc
     3301 ccccgtcgtg attcactccc gcgccgcgcg gctggccgca ctcgccgcgc tctgtgccgc
     3361 cgcgcgcctc gacgcggggc cgcgcgtccc agcggtcgcc tcgtacacga tcgaagcgtc
     3421 cgtgaacgac gagcacgtcg tcgcgggcaa ggagaccgtc cggttcacga accgcacgcg
     3481 gcacaccttc accgacctcg cactccacct ctacctcaac gcgtggcgga acgaccgctc
     3541 gacctggctc gtcgagcagg cccgcggcgg cgggcgcgcg gaccggggcc tcgccgcgaa
     3601 agactcggcg cgctggggct ggtcggagat ccaccggatc gccctcgacg acggaacgga
     3661 cctgacgtcc ggcctcacgt acgtctcccc cgacgacggc aacgcggacg accgcacgct
     3721 cgtctcggtt ccgctgccgc ggcccgtcgc cccgggcgag acgctcgtct tccgggtgga
     3781 ctgggaggcg aggttcccgc gcgccgtggc gcggaccggc tggaaggacg actacatcct
     3841 cggggcgcag tggttcccga agctcggcgt cgcgacggac gccggctgga acgcccacca
     3901 gttccacgcc ggcaccgagt tcttcgcgga cttcggcgac tacgacgtga cgctctcgct
     3961 gcccgcggcg aacaaagggc acatcggcgg aaccggcgtc ctcaaggagg agacggagct
     4021 cgcgggcggc ctcgtgcgcg cgcgcttcgc cgccgaggac gtccacgact tcgcgttcgc
     4081 ggcctgtccg cgtttcgtcg tcgtgcgtga cacgttcagc gcgaagggcc tcccgaacgt
     4141 cgacatcgtc ctcctccttc agcccgacca ccggccgctg cgcgagcggt acctcaaggc
     4201 gacgaaggtc ggcctggagg acttcgggac gcggtacctt ccgtacccct acccggtcgt
     4261 caccgtggtg gatcctccgt ggggcagcca cacggagggg atggagtacc cgaccctttt
     4321 cgtgggcggc gggcgtgaga tcgcgccgaa gaaggcccac tcgcccgaga gcgtgacgat
     4381 ccacgagtac ggccaccagg tgttctacgg gatgctcgcc tcgaacgagg tcgaggaggc
     4441 gcacctcgac gagggcttca ccacttacgc gacgacgaag gcgctcgagg ccgcgtacgg
     4501 gaacccctcc ctcgtcgtga ggttctacgg cctgcccgtg acgtttgcgg gcgtgacggt
     4561 gccgtatccc ttcgggacga cggaccgctt ccaccgctgg cagctcgccg cgcgctcgga
     4621 cgcgcaggcc gtcccctcct accggcagtt gaacggcgac gcggtccgca acaatgcgta
     4681 cctccgcacg acgctcctcc tccgttcgtg cgagcggacg ttcggcgagg gcgtctgggc
     4741 gaaggtcatg aagacgtacg cgacgcgctt cgccttcaag cacccggcga ccgccgattt
     4801 cctcggcgtc gtgagagagg tcgcgggcgc gggcgcggcg gagtcggtcg cggggatcgt
     4861 ggcgacggcg ggctccgtcg actacgccgt gacggccgtg gagacccacg aggcgtcggg
     4921 cccgaccggc tttacgggcg agggccccga gcggaagttc gaggccgcga agaagggcgg
     4981 aacgggcgcg tacgaatcca ccgtcgtcgc ccagcgcttc ggcgaggcgg tctggcccgt
     5041 ggacgtcgtc ttcacgttcg cggacggctc gacgctgacg cggccgtggt ccggcgcgga
     5101 gtcgtgggtg cgctggaggc tccgcgggcc gaagctcgtc tcggccgagg tcgaccccgc
     5161 gcgcaagtgc ctcctcgacg gcaacacgct gaacaacggg cgccgcacgg agcccgacgc
     5221 gcgcgcctct cgcgcgtgga cggcgcgctt catgttctgg gcgcagaacc tcctcgaggc
     5281 gttctccctg ctcgggtggg cggcgttccc gtgaggcgcg actccgtcct cgtcctcgcc
     5341 gcgcgcggcg ccctgtcggc gctcgggacc tggaaggcgc tcctcctcgc gctcgtcctc
     5401 aacctcctcc tcgcgtacgc gttcgcgcac cccgtgaacg cggcgctgcg ccaggcgctg
     5461 gacaagagcc cgtgggcggc ccggctcgcc gcggcgaacg cacccctctt cgagttctac
     5521 ggggagatct cccgggcccg gccggacgtc ttcggcgacc tgtccgcgtg ggacgcgatc
     5581 gcgaccggcg acgaaggcga gcggagcgcg gcgcggcgcg cccccctgtc cggcttcctc
     5641 agcgcgaccg gcgacttcga tcacgacggc atcagcgacc tcgcggtgac caaccg
//
LOCUS       DAOMFY010000259         5695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4835_length_5695_cov_10.596631, whole genome shotgun sequence.
ACCESSION   DAOMFY010000259 DAOMFY010000000
VERSION     DAOMFY010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4835_length_5695_cov_10.596631"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..491
                     /locus_tag="PLB01_16160"
     CDS             30..491
                     /locus_tag="PLB01_16160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQQ78889.1"
                     /translation="MSSRARRIPTVALTAGYKRTFGLDTGLAGLVIPLPIFDANAGNV
                     ARAEAEEQAAQSRLTALESRLLAGGAAQIAAARELRARALTVEEDLVRPAEGARDAAT
                     AAFREGASDVLRLVDARRLYLDARREALDLFAEAALRAAEVRLLLGEEAIP"
     gene            488..1750
                     /locus_tag="PLB01_16165"
     CDS             488..1750
                     /locus_tag="PLB01_16165"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ78890.1"
                     /translation="MSESPGPERTLWQRVLGRPQFRRARLLVLALVLLVPCVVLVRWL
                     TRKAPETGPEKPAEAGAVVLGESARRDAGIAVETVKRMARTDRLEAPGVLALDEARTA
                     RIGSLVEGVVLSVNAEVGDRVTAGQRLAHLNSRVIHDAWADYRKAIAERRRRETELAY
                     ASASAGRADRLLEARAISVQERQRADADRVAAAEELDRAKTEVRRSEEALEHLGITSG
                     EDPSGEKGEDIPVKAPLAGVVLERKISTGTAVTPGMPLFVVSDLASLWALAEIDETKL
                     PLVKAGETAEIRVSAWPGEVFRGRVAFVGDAVNPKTRRVMVRCQVSNAGGRLKPEMYA
                     SIAMGEGAPRSILAAPARAVQEMEGKDIVFVPGAAGRFTQREVTIGTEAEGWVEVSSG
                     LKEGETVASDGSFLLKSELLKSTERPGN"
     gene            1752..4886
                     /locus_tag="PLB01_16170"
     CDS             1752..4886
                     /locus_tag="PLB01_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQQ78891.1"
                     /translation="MGLIERVVAASLRQKVFVLLCLAALVGGGLWARRSIGVEAFPDL
                     TNNQVIVVTEAPGLGAVEVEQRVTYPVETALTGAPGATEVRSLSKFGLSMVTVVFEDS
                     VPVYFARQLVTERLAEARGRIPQGLEPSLGPVATAFGEIYQYLVEGDGADLMEKKTLQ
                     DWEIRPRLRSVPGVSELNSWGGLTRQFHVVVDPRKLEKYGLSLRQLFDAVAENNVSFS
                     GGFIEHRSERFTVRGVGLVRGVEDLRRLVVASVEGVPVFVGDVAQVSVGAMPRHGAVT
                     RDGRGESVAGMVIMLKGENGKTVAGRVKARVKEIVSSLPKGLSIVPFYDQTEVIDRTS
                     HTVQKNLLEGSLLVVLVLFFFLRDLRAALLVAFVIPVAMLAAFLGMKVFGVSANLMSL
                     GAIDFGLIVDGAVVMVENIIRRREERGDPVGPGRESPTSFFTSAATEVARPILFGVLI
                     IIAVYIPIFALEGLEGKMFRPMAVTVCSALVGALAVSLTAVPVLASLVLPVHAAEQPE
                     EWFRRLRRTYVAHLDAAMGRRRRTLTVALVVVAAAIASVPFLGTEFMPRLDEGSILIE
                     TRKLPSVSLPESAAISGRVEQILMRFPEVRQVVTKIGRPDVATEAMGIYQGDVYVVLH
                     PREAWKTGRTKEHLVDAMARALAEMPGLSANFTQPMAMRLDEVVSGVKADVAVKIFGP
                     DAAVLEALGEDVRKALEPIRGAADLQVEALTGAAQIQVDVDREAMARYGLNVSDLRQV
                     VETAVGGAVATEILDGPRRFGVLVRFPDALRADRAALEALLLSAPGGEKVPLGTVARV
                     AAVRGPEAINHESGQRRLVVQTNVRGRDMGSFVAEGKVRIAAAVKAPPGYHFEWGGQF
                     ENQERAMKRLVVVIPLSLVIIFLLLFITFGMVRQALLVLVNVPFALVGGIGALWLRGL
                     NLSLSAAVGFIALFGVAVLNGVVLVTAINHRREAGASLRAAIVGAAGTRLKPVLMTAL
                     VAALGFIPMALSSGAGSEVQRPLASVVIGGVVTSMLLTLIVLPTLYDIVEERVARRRQ
                     EEFYADAAEDEIPT"
     gene            complement(4904..>5695)
                     /locus_tag="PLB01_16175"
     CDS             complement(4904..>5695)
                     /locus_tag="PLB01_16175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ78892.1"
                     /translation="VVFEPAAGEQPGERLTRLGVLLRELQTRKLLFLHRPGGIRQGGV
                     LVPLVNLTTDAAALVASKELSRKERLILEGARQLCEEAAPLPFTVAITSPLNVLRELF
                     TVKGAGTLLRRGARIVRREGWDGVDVGRLRELLASSFGRAPLDAFFETTPAHVYLEEA
                     YRGCAILVDTPLGAYLTKFAVSPEAQGEGIARDLWEAVVADAPVVFWRARRTNPIRDW
                     YDKLCDGLVRTPDWTVYWKGLAPGEIPQAIDWAMRQPVDIPRDEE"
BASE COUNT          795 a         1959 c         2079 g          862 t
ORIGIN      
        1 caagatcgcc gtcgacgccg gctatcacat tgagcagccg agcgcggcgc atcccgacgg
       61 tcgcgctgac ggccggctac aagaggacct tcggcctcga caccgggctc gcgggcctcg
      121 tgatcccgct gccgatcttc gacgcgaacg ccggcaacgt cgcccgcgcc gaagcggagg
      181 aacaggcggc gcagtcgcgg ctgacggccc tcgaatcgag actcctcgcc ggtggtgcgg
      241 cgcagatcgc cgcggcgcgc gagctgcgcg cgcgcgccct cacggtcgag gaggatctcg
      301 tccggccggc ggagggagcg cgcgacgcgg cgaccgcggc cttccgcgaa ggggcctcgg
      361 acgtcctccg cctcgtcgac gcgcgccggc tctatctcga cgcgcgccgc gaggcgctgg
      421 acctgttcgc cgaggcggcg ctccgagccg ccgaggtgcg cctgctgctc ggagaggagg
      481 caattccatg agcgagagtc ccggaccgga gcggaccctg tggcagaggg tgctgggccg
      541 tccccagttc cgccgcgcgc gcctcctggt cctcgccctg gtccttctcg tcccgtgcgt
      601 ggtcctcgtg agatggctga cgcggaaggc gccggagacg gggcccgaaa agcccgccga
      661 ggccggagcc gtcgtcctgg gcgagtcggc gcggcgcgac gccgggatcg ccgtggagac
      721 ggtcaagcgc atggcccgca cggacaggct cgaagccccg ggcgttctcg cgctcgacga
      781 agcccggacg gcgcgcatcg gctcgctcgt ggagggagtc gtgctctccg tgaacgccga
      841 agtcggcgac cgcgtcacgg ccggacagag gctcgcacac ctcaacagcc gcgtgatcca
      901 cgacgcctgg gccgactacc ggaaggcgat cgccgagcgc aggcggcgcg agacggagct
      961 cgcctacgcg tcggcgtcgg ccggacgggc ggaccggctc ctcgaggccc gggcgatctc
     1021 ggtccaggag cggcaacggg ccgacgccga ccgcgtcgca gcggccgagg agctggaccg
     1081 tgcgaagacc gaggtccggc ggtccgagga ggctctcgag cacctcggca tcacgtccgg
     1141 agaggacccc tcgggcgaaa aaggcgaaga catcccggta aaggcgccgc tggcgggcgt
     1201 cgtcctcgag cggaagatct cgacgggcac ggccgtgacg ccgggcatgc cgctcttcgt
     1261 cgtcagcgat ctcgccagcc tttgggccct cgccgagatc gacgagacga agcttcctct
     1321 cgtgaaggcg ggcgagacgg cggagatccg agtctcggcc tggccgggag aggtcttccg
     1381 cgggcgcgtt gcgttcgtgg gcgacgcggt caatccgaag acgcggcgcg tcatggtccg
     1441 ttgccaggtc tcgaacgcgg gtggccgcct caagccggag atgtacgcct ccatcgcgat
     1501 gggagaaggg gcgccgcggt ccattctcgc cgcccccgct cgcgccgttc aggagatgga
     1561 agggaaggac atcgtcttcg ttcccggcgc cgcaggacgg ttcacgcagc gggaggtgac
     1621 gatcgggacc gaggccgagg gctgggtcga ggtctcctcg gggctcaagg agggagagac
     1681 cgtcgcctcg gacgggagct tcctcctgaa gtcggagctt ctgaagagca cggagcgccc
     1741 ggggaactga gatgggattg atcgagcggg tcgtcgcggc ctctctccgg cagaaggtgt
     1801 tcgtgctgct ctgcctggcc gcgctcgtcg gcggaggact ctgggccagg cgctccatcg
     1861 gcgtggaggc gtttccggac ctcacgaaca accaggtcat cgtcgtcacc gaagcgccgg
     1921 ggctcggtgc cgtcgaggtc gagcagcggg tcacgtatcc cgtggagacg gcgctcacgg
     1981 gggcgcccgg cgcgacggag gttcgctctc tctcgaagtt cgggctttcg atggtcacgg
     2041 tcgtcttcga ggacagcgtc ccggtctact tcgcgcgaca gctcgtgacc gagcggctcg
     2101 ccgaggcgcg gggccggatt ccgcagggcc tcgagccgtc gcttggcccg gtcgccacgg
     2161 cgttcggcga gatctaccag tacctcgtcg agggggacgg cgcggacctc atggagaaga
     2221 agacgcttca ggactgggag atacggccgc ggcttcgctc ggtgccgggc gtctccgaac
     2281 tgaactcctg gggcgggctg acgcggcagt tccacgtcgt cgtcgacccg cggaagctgg
     2341 agaagtacgg gctgtcgctc aggcagctct tcgacgcggt ggcggagaac aacgtctcct
     2401 tctccggcgg cttcatcgag caccgttcgg agcgcttcac ggtccgcggc gtcggtctcg
     2461 tccgcggcgt cgaggacctg aggcggctcg tcgtcgcgtc cgtggagggc gtgccggtct
     2521 tcgtcggcga cgtcgcgcag gtgagcgtcg gcgcgatgcc gcggcatggc gcggtcacgc
     2581 gcgacgggcg cggcgagtcg gttgcgggca tggtcatcat gctgaagggc gagaacggga
     2641 agaccgtggc cgggcgggtc aaggcgcgcg tgaaggagat cgtgtcgagc ctgccgaagg
     2701 ggctctcgat cgtccccttc tacgaccaga cggaggtgat cgaccggacg tcgcacaccg
     2761 tccagaaaaa cctcctcgaa ggctcgctcc tcgtcgtcct cgtcctcttc ttcttcctcc
     2821 gcgaccttcg tgcggcgctg ctcgtcgcct tcgtcattcc cgtcgcgatg ctcgcggcct
     2881 tcctcgggat gaaggtcttc ggcgtctcgg cgaacctgat gtcgctcggc gcgatcgact
     2941 tcggcctcat cgtggacgga gccgtcgtca tggtcgagaa catcatccgc aggcgcgaag
     3001 aacgcgggga cccggtcggg cccggccggg agagcccgac ctccttcttc acgtcggccg
     3061 ccaccgaggt cgcccgcccg atcctcttcg gcgtcctcat catcatcgcg gtctacattc
     3121 cgatcttcgc cctcgagggg ctcgagggaa agatgttccg gcccatggcg gtcacggtct
     3181 gctcggccct cgtcggggcc ctcgcggtgt cgctgacggc cgtgcccgtc ctcgcgtcgc
     3241 tcgtcctccc cgtgcacgca gcggagcagc ccgaggagtg gttccggaga ctccgccgga
     3301 cgtacgtcgc tcacctcgac gcggcgatgg gccgcaggcg gcggacgctc acggtggcgc
     3361 tcgtcgtcgt cgcggccgcg atcgcttcgg tgcccttcct cgggaccgag ttcatgccgc
     3421 gcctcgacga ggggtcgatc ctcatcgaaa cgcgcaagct gccttccgtc tcgctccccg
     3481 agtccgccgc gatctcgggc cgcgtcgagc agatcctgat gcgctttccg gaggtccggc
     3541 aggtcgtcac gaagatcggg cgccccgacg tcgcgacgga ggcgatgggc atctaccagg
     3601 gggacgtcta cgtcgtcctg cacccgcgcg aggcctggaa gaccggccgg acgaaggagc
     3661 atctcgtgga cgcgatggcc cgggccctcg cggagatgcc ggggctctcc gcgaacttca
     3721 cacagccgat ggcgatgcgc ctcgacgagg tcgtctccgg tgtcaaggcg gacgtggccg
     3781 tgaagatctt cggtcccgac gcggccgtcc tcgaggccct cggcgaggac gtgcgcaagg
     3841 cgctcgagcc gatccgcggc gccgccgacc tccaggtcga agccctgacg ggcgccgcgc
     3901 agatccaggt cgacgtggac cgcgaggcga tggcgcgcta cggcctgaac gtctcggacc
     3961 tccggcaggt ggtcgagacg gcggtcggag gggcggtggc gacggaaatc ctggacggcc
     4021 cgcgccgctt cggcgtcctc gtgaggttcc cggacgcgct gcgcgccgac cgggccgcgc
     4081 tcgaggcgct cctcctctcg gcgccgggcg gggagaaggt ccccctcgga acggtcgcgc
     4141 gcgtggcggc cgtgcggggt cccgaggcga tcaaccacga gtcgggacag cgccgtctcg
     4201 tcgtgcagac gaacgtgcgc ggacgcgaca tggggagttt cgttgccgag ggaaaggtcc
     4261 gcatcgcggc ggccgtcaag gctcctccgg gctatcactt cgaatggggc ggccagttcg
     4321 agaaccagga gcgtgcgatg aagcggctcg tcgtcgtgat cccgctgtcg ctcgtcatca
     4381 tcttcctgct tctcttcata accttcggga tggtgcggca ggcgctcctc gtcctcgtga
     4441 acgtcccgtt cgccctcgtg ggcggcatcg gcgcactgtg gctccgcggg ctcaacctga
     4501 gcctgtcggc ggccgtcgga ttcatcgcgc tcttcggcgt ggcggtcctg aacggcgtgg
     4561 tcctcgtgac ggcaatcaac caccggcgag aggcaggcgc gtcgctccgc gccgcgatcg
     4621 tgggcgccgc gggaacgcgc ctgaagccgg tcctcatgac ggccctcgtc gcggccctcg
     4681 ggttcatccc gatggccctc tccagcgggg ccggatcgga ggtccagcgg ccgctcgcga
     4741 gcgtcgtgat cggcggcgtc gtcacctcga tgctcctgac actcatcgtc cttccgacgc
     4801 tgtacgacat cgtcgaggaa cgcgtggcgc gccgccggca agaggagttc tacgcggacg
     4861 ccgccgagga cgaaatcccg acctgaaacg gccgcgggcg cggctactcc tcgtcgcgcg
     4921 gaatgtccac cggctgccgc atcgcccagt cgatcgcctg cgggatctcg ccgggggcaa
     4981 ggcctttcca gtagacggtc cagtccggcg tcctcacgag gccgtcgcag agcttgtcgt
     5041 accagtcgcg gatcggattg gtccggcgcg cgcgccagaa cacgacgggc gcgtcggcga
     5101 cgacggcctc ccacaggtcg cgcgcgatgc cctcgccctg cgcttccggc gacacggcga
     5161 acttcgtgag gtaggcgccg agcggcgtgt cgacgaggat cgcgcagccg cggtaggcct
     5221 cctccaggta gacgtgcgcg ggcgtcgtct cgaagaaggc gtcgaggggc gcgcggccga
     5281 agctcgacgc gagcagctcc cgcaggcggc cgacgtccac gccgtcccag ccctcgcggc
     5341 gcacgatgcg cgctccgcgg cgcaggagcg tgccggcgcc cttcacggtg aacagctccc
     5401 ggaggacgtt gagcggcgac gtgatcgcga cggtgaacgg gagcggcgcg gcctcctcgc
     5461 agagctgccg cgccccttcg aggatgagcc gttccttgcg cgagagctcc ttgctcgcga
     5521 cgagcgcggc cgcgtcggtc gtgaggttca cgagcgggac gaggacgccc ccctgccgga
     5581 ttcctcccgg gcggtggagg aacaggagct tccgcgtctg gagctcgcgg aggaggacgc
     5641 cgagccgcgt caggcgctcg ccgggctgct cgcccgccgc cggctcgaag acgac
//
LOCUS       DAOMFY010000260         5694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4838_length_5694_cov_7.192765, whole genome shotgun sequence.
ACCESSION   DAOMFY010000260 DAOMFY010000000
VERSION     DAOMFY010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4838_length_5694_cov_7.192765"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..191)
                     /locus_tag="PLB01_16180"
     CDS             complement(69..191)
                     /locus_tag="PLB01_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78893.1"
                     /translation="MADEPKDAPATPPAGDAPKPASDAAKPAEKAAGGAKRVEF"
     gene            complement(184..687)
                     /locus_tag="PLB01_16185"
     CDS             complement(184..687)
                     /locus_tag="PLB01_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQQ78894.1"
                     /translation="MGLIENAFPDNVLTTTVDALIGWGRKNSLWPMQFGLACCAIEMM
                     AVVDSRHDMARFGAEVFRGSPRQCDMMLVAGTVTEKMAPIIKRLYDQMADPKWVIAMG
                     SCATCGGPYRSYAVTQGVDRIIPVDVYVPGCPPRPEALLYGLMELQKKIQKMRVLRRA
                     PEELAVG"
     gene            complement(678..1055)
                     /gene="ndhC"
                     /locus_tag="PLB01_16190"
     CDS             complement(678..1055)
                     /gene="ndhC"
                     /locus_tag="PLB01_16190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQQ78895.1"
                     /translation="MNPSNPSMASYLPILYFAIMAAIVPVAGLAAMSLLRPKNPNPTK
                     LSPYECGVASPTEAFDHRFSVRYYLIAVLFVVFDVETIFLFPWAVMYHKLKLFGLVEM
                     LVFLVILVVGYVYAWRRGALSWA"
     gene            complement(1162..2331)
                     /locus_tag="PLB01_16195"
     CDS             complement(1162..2331)
                     /locus_tag="PLB01_16195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQQ78896.1"
                     /translation="MMPLAQRLAERIRAGGPLRFDAFQEAALYDAEGGYYERGGRVGR
                     SGDFVTGPSWHPAFGRTIARIARRLREEGKRREEEKISSKGEEGLPSATRAATDVVDV
                     GAGEGELLAAADSALQEWGIRDQFRFVGVERSAARRAKAAEICPGATWLSALEEIPSS
                     SLSGLVVAYELFDALPVRALFFEGEKLLERVVTLAPGGEGFAWGLADCSDGTELLERF
                     RERGIHLQRNQLLEVRAGAAGLASALASRLLSGIILVFDYGAPARALYSAARANGTLE
                     AFVSHAVTRDVLTDPGSRDLTAWVDFSELADAFAAAGLSVEGPVSQSRVLAAGGIFEE
                     LTVDPDAPLDAAREADRRAAGALVQPGGMGESIRVLSASRGSALGSALASWTRPA"
     gene            2390..2809
                     /locus_tag="PLB01_16200"
     CDS             2390..2809
                     /locus_tag="PLB01_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78897.1"
                     /translation="MTYGISETPSGLTIALAAKTRDELYGDAVKAALEAVYGGEPPAG
                     ESDGQTYPVQGAGLDDATMLGTLFEECFVAARRADGTLHPPRWLSFDEGRVTANLALS
                     TPKTPARSLTPALHGAVETSGAFPDFRATLAFALPSD"
     gene            complement(2801..3868)
                     /gene="gmd"
                     /locus_tag="PLB01_16205"
     CDS             complement(2801..3868)
                     /gene="gmd"
                     /locus_tag="PLB01_16205"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQQ78898.1"
                     /translation="MSTAMRALITGITGQDGSYLAELLLGKGYEVHGIIRRSSSFNTE
                     RLDHLYQDPHVPKRNLVLHYGDLNDASSLNRVLRLVEPHEIYNLGAQSHVKVSFDVPE
                     YTGEVTALGATRILEAVREVGLRPRFYQASSSEMFGKVAETPQRESTPFHPRSPYGVA
                     KLYAHAITVNYRESYGLHATSGILFNHESPRRGETFVTRKITRAVGRIVCGQQEVLWL
                     GNLDAQRDWGFAGDYVQAMWLMLRQDEPDDFVIATGETHTVREFCDRAFARAGRPLRW
                     TGSGADETGVEAATGIPRVQVDPRYFRPAEVDLLLGDPSKAKRVLGWTPRLAFAGLVD
                     MMVDADLARARQEAAASVPSV"
     gene            4052..4957
                     /locus_tag="PLB01_16210"
     CDS             4052..4957
                     /locus_tag="PLB01_16210"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQQ78899.1"
                     /translation="MRRTAVTVLAFLAASWSGRLAAQVPIREIRLTLEPTTYNGPCPT
                     QLRAHIVFVSNYPARVDEDNWGWNFGRLAPHSWDVPGGHLRTSGRETVVDTTLTLPRA
                     GDTAGGLVHEGSVHVIMELAPTISNKVAYSVRCTPTVRIDPNAVKPVQPAPGVSLQKV
                     PTIPPPSGGGAGPAVHLPDLVVVGLEPVAGNVVVTNSGTGPAGPSELGIRVRCLGDDG
                     EGKSCTGRFAWPPGVVVDPALGGPTLPVPALAPGVSRTIPVPSWAAIKFTPGKYELVA
                     TVDARNAAAESNEANNTRAVRFTRP"
     gene            complement(5017..5133)
                     /gene="rrf"
                     /locus_tag="PLB01_16215"
     rRNA            complement(5017..5133)
                     /gene="rrf"
                     /locus_tag="PLB01_16215"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5212..>5694)
                     /locus_tag="PLB01_16220"
     rRNA            complement(5212..>5694)
                     /locus_tag="PLB01_16220"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          943 a         2006 c         1870 g          875 t
ORIGIN      
        1 gcgtcaagtc gaccgtcccc gacgccgcgt cgcccagcgg cgtggcgcgc tccaacgggg
       61 ccgcgggttc agaattccac ccgcttcgct ccacccgcag ccttctcggc gggcttcgcc
      121 gcgtcggatg cgggcttggg agcgtctccg gcgggcggcg tggccggggc gtccttcggt
      181 tcgtcagcca acggcgagct cctcgggagc gcgccggagc acgcgcatct tctggatctt
      241 cttctggagc tccatcaggc cgtagagaag ggcctcgggc cgcggcgggc agccggggac
      301 gtagacgtcc acggggatga tccggtcgac gccctgcgtg accgcatagc tccggtacgg
      361 gcctccacac gtcgcgcacg agcccatcgc gatgacccac ttcgggtcgg ccatctggtc
      421 gtagaggcgc ttgatgatcg gcgccatctt ctcggtgacg gtgcccgcga cgagcatcat
      481 gtcgcactgc cgtggagagc cgcgaaagac ctcggcgccg aaacgcgcca tgtcgtggcg
      541 gctgtcgacg acggccatca tctcgatcgc gcagcacgcg aggccgaact gcatcggcca
      601 gagagagttc ttgcggcccc agccgatcag ggcgtcgacc gtggtcgtga ggacgttgtc
      661 cgggaacgcg ttctcgatca ggcccacgaa agcgctcccc tccgccacgc gtagacgtat
      721 cccacgacga ggatcacgag aaagacgagc atttccacga ggccgaacag cttcagcttg
      781 tggtacatga ccgcccacgg gaacaggaag atcgtctcga cgtcgaacac gacgaagagg
      841 accgcgatca ggtagtagcg gaccgagaag cggtggtcga acgcctccgt cggggacgcg
      901 acgccgcact cgtacggcga gagcttcgtc gggttcgggt tcttcggcct caggagcgac
      961 atcgccgcga ggcccgcgac cgggacgatc gccgccatga tggcgaagta caggatgggc
     1021 aggtaagacg ccatggacgg attggacggg ttcatgagca cacccctcgc ggtccgcccg
     1081 cgcctgcgcg ccgcccgggt tcgaacaaga cgagggggat tctctgtgcc gaccccggcg
     1141 aagtcaaacg atgcaatggc atcacgccgg gcgcgtccag gacgcaaggg ccgagcccag
     1201 cgcagacccg cgggacgcgc tcaggacccg gatcgactcg cccattcccc cgggctggac
     1261 gagagcgccg gcggcccggc ggtccgcctc ccgcgccgcg tcgagcggcg cgtccgggtc
     1321 caccgtgagc tcctcgaaga tccctcccgc cgcgaggacg cgcgactggc tgaccggtcc
     1381 ctcgacggag agcccggctg cggcgaacgc gtcggccagc tccgaaaagt cgacccacgc
     1441 cgtcaggtct cgcgacccgg ggtccgtgag aacgtcgcgc gtgacggcgt gggagacgaa
     1501 cgcctcgagc gtcccgttcg cgcgcgccgc cgaatagaga gcgcgcgccg gcgcgccgta
     1561 gtcgaagacg agaatgatgc cggacaagag acgcgaagca agggccgaag cgaggcctgc
     1621 cgcgccggcg cgcacctcga gcagttgatt cctctgaagg tgaatgcccc tctctcggaa
     1681 ccgttcgagc aattcggtgc cgtcgctgca atccgcaagg ccccacgcga agccctcgcc
     1741 gccgggcgcc agcgtcacga cgcgctccag cagcttttca ccttcgaaga aaagcgccct
     1801 caccggcagc gcatcgaaca gctcgtaggc gacaaccaat ccagagagag aagaagacgg
     1861 gatttcttcg agtgcagaaa gccaggtggc tccggggcaa atctccgcgg ccttcgcgcg
     1921 ccgggccgcg gagcgttcga cgcccacgaa gcgaaactgg tcgcggatcc cccactcctg
     1981 aagcgcagaa tccgcggccg cgagcagctc gccctccccg gctccaacat ccacgacatc
     2041 cgtcgccgcg cgtgtcgccg acggaagccc ctcttcccct ttcgaagaaa tcttctcttc
     2101 ttctcttctc tttccctctt cccgaagccg ccgcgcgatc ctcgcgatcg tccgcccgaa
     2161 ggccggatgc cacgagggac cggtcacgaa gtcgccggag cgcccgacac gccccccgcg
     2221 ctcgtagtag cccccctcgg cgtcgtagag ggccgcttcc tggaacgcgt cgaagcggag
     2281 cgggcccccg gcgcggatgc gctccgcgag acgctgagca agcggcatca tcgccgccga
     2341 gactagcagc cggttaagat cttcgttcgg accgaggagg atcgaagacg tgacgtacgg
     2401 aatctccgag acgccctccg gactcacgat cgcgctcgcc gcgaagaccc gcgacgagct
     2461 ctacggcgac gcggtcaagg cggccctcga ggccgtctac ggcggcgagc cgccggccgg
     2521 cgaatccgac ggccagactt atcccgtgca gggggcgggg ctcgacgacg ccacgatgct
     2581 cgggacgctt ttcgaagagt gtttcgtggc cgcgcgccgg gcggacggca cgctccatcc
     2641 gccgcgctgg ctctcgttcg acgagggccg cgttaccgcg aatctcgcgc tttcgacgcc
     2701 gaagacgccc gcgcggtcgc tgacccccgc gctccacgga gcggtcgaga cgtccggcgc
     2761 gttccccgac ttccgcgcga cgctggcgtt cgcccttccc tcagactgag ggcacggacg
     2821 ccgcggcttc ctggcgggcc cgcgcgaggt ccgcgtccac catcatgtcg acgaggcccg
     2881 cgaacgcgag gcgaggagtc cagccgagga cgcgcttcgc cttcgaagga tcgccgagaa
     2941 gcaggtccac ttccgccggc cggaagtagc gcgggtcgac ctggacgcgg ggaatgcccg
     3001 tcgcggcctc gacgcccgtc tcgtccgcgc cggaaccggt ccagcgcaga ggcctgcccg
     3061 cgcgcgcgaa cgcgcggtcg cagaactcgc ggaccgtatg cgtctctccg gtcgcgatca
     3121 cgaagtcgtc cggttcgtcc tggcgcagca tgagccacat cgcctggacg tagtcgcccg
     3181 cgaagcccca gtcgcgctgc gcgtcgaggt tcccgagcca gaggacctcc tgctggccgc
     3241 agacgatccg gcccacggcg cgcgtgatct tgcgcgtcac gaacgtctcg ccgcgcctcg
     3301 gcgactcgtg gttgaagagg atgccgctcg tcgcgtggag accgtaggac tcgcggtagt
     3361 tgaccgtgat cgcgtgcgcg tagagctttg cgactccgta cggcgaccgg ggatggaagg
     3421 gcgtcgattc cctctgcggc gtctcggcga ccttgccgaa catctccgac gacgatgcct
     3481 ggtagaagcg tggtctgagg ccgacttcgc gcacggcctc gaggatgcgc gtggcgccga
     3541 gcgcagtgac ctcgcccgtg tattccggca cgtcgaacga caccttcacg tggctctggg
     3601 ccccgaggtt gtagatctcg tgcggctcca cgagccgcag gacgcggttc agcgaggagg
     3661 cgtcgttgag gtcgccgtag tgcaggacga ggttccgctt cgggacgtgc ggatcctggt
     3721 agaggtggtc caggcgctcg gtgttgaacg aggaggagcg ccggatgatc ccgtggacct
     3781 cgtacccctt cccgagcagg agctcagcca gatagctgcc gtcctgaccc gtgattcccg
     3841 tgatgagagc gcgcatggcg gtcgacatcg ggtcgagtgt agcggcccgg cgcgaggagg
     3901 ggaacaccgg gaaaccgtga ttcctctcac gttggcgccc tcggacgagc ggttggcgcg
     3961 ccccggccag atcctcgcct tgaccgtccc ccgccgccgg accatcgtgt ccacgccccg
     4021 agcgcccggc tgccgcgaag gagattcacc gatgagacga accgccgtca ccgtcctggc
     4081 gttcctcgcc gcttcctggt cgggccgcct tgcggctcag gtcccgatcc gcgagatccg
     4141 actgaccctc gagccgacga cgtacaacgg cccctgcccc acacagctcc gcgcgcacat
     4201 cgtgttcgtg tccaattacc cggcccgcgt cgacgaggac aactggggct ggaacttcgg
     4261 acgcctcgcg ccgcacagct gggacgtgcc gggaggccat ctccgcacct cgggccgcga
     4321 gaccgtggtg gatacgacgc tgaccttgcc gcgcgcgggc gacaccgccg ggggcctcgt
     4381 ccacgagggg tccgtccacg tcatcatgga gctcgcgccc acgatctcga acaaggtcgc
     4441 gtattcggtc cgctgcactc cgaccgtgag gatcgatccg aacgccgtga agcccgttca
     4501 gccggctccg ggcgtctcgc tccagaaggt cccgacgatt ccaccgcctt ccggcggagg
     4561 ggcggggccc gccgtccatc tccccgacct cgtcgtggtc ggactggagc cggtcgcggg
     4621 aaacgtcgtc gtgacgaact ccggcaccgg gccggcaggg ccctccgagc tcgggatccg
     4681 cgtccgctgc ctcggagacg acggggaggg aaagagctgc acgggccgct tcgcgtggcc
     4741 gccaggagtc gtcgtggacc cggcgctcgg cgggccgaca ctccccgttc cggcgctcgc
     4801 gcccggagtg tcgcggacga tcccggttcc ctcgtgggcc gccatcaagt tcacgccggg
     4861 gaagtacgag ctcgtcgcga ccgtcgacgc gcgaaacgcg gccgcggagt cgaacgaagc
     4921 gaacaacacc cgcgcggtcc gcttcacgag gccctgatcg ccggcacccc aagaaaagac
     4981 aagggcccgg acctttcggt ccgggccctt caaacttccc ggcagcttcc tactctccca
     5041 cacagtttcc cgtgcagtac catcggctcc gaggggctta acggccgtgt tcggaatggg
     5101 aacgggtgga gccccctcga taaggccacc ggaaactcta tttcgtttcc gtacagaatc
     5161 gagcgacggc aagtgcgaat gtctctcgta cgttcgcgcc tcaagaattt tatggccaag
     5221 ccgcacggac gattagtacc ggtcagcttc gcgcctcgca gcgcttccac acccggccta
     5281 tcaacgtggt catctcccac ggtccttcag ggggcttgcg ccccggggaa atctcatctt
     5341 ggggtcggct tcacgcttat atgccttcag cgtttatcca aaccgaactc cgctacccgg
     5401 cgtgtgccgc tggcgcgaca accggtgcac aggaggttcg tccaacccgg tcctctcgta
     5461 ctagggtcgg ggccccgcaa atttcctgcg cccacacgag atagggaccg aactgtctca
     5521 cgacgttctg aacccagctc acgtaccgct ttaaccggcg aacagccgga cccttgggac
     5581 ctgcttcagc cccaggatgc gatgagccga catcgaggtg ccaaaccttg ccgtcgatgt
     5641 gaactcttgg gcaagatcag cctgttatcc ccggcgtacc ttttatccgt tgag
//
LOCUS       DAOMFY010000261         5688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4848_length_5688_cov_6.689686, whole genome shotgun sequence.
ACCESSION   DAOMFY010000261 DAOMFY010000000
VERSION     DAOMFY010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4848_length_5688_cov_6.689686"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PLB01_16225"
     CDS             complement(<1..1017)
                     /locus_tag="PLB01_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78900.1"
                     /translation="MEVRDRRAGLWPVLLALLPLLPSWWFAILKPWPPMSVDDDGAVI
                     EMAERRALHGKQLTGVYSRFGFSHPGPVQFYLMAPVYAATGQRSAGLSLAALLLTTLF
                     ACAAAWSAARILGLRRGLLVAAGLALLLARMGPGWVAHAWGPHAVVVPFALLVVLALG
                     LARQGAAWLPGVAFVATFLVQTHLGTAPAVACVVAVAFALAGRLGRRLVAMKPLALSI
                     VLLAALWTPPLLEQLRGTPEHPGNMTLLWRFFRTHGARHTFVEVFGPLAHELGSIPVA
                     LASAVVPSTTDHRDVGAGLFTLLLVALLPLALAAARRRRDDDAAALRVLQPNDPDSYY
                     ASREL"
     gene            1073..2161
                     /locus_tag="PLB01_16230"
     CDS             1073..2161
                     /locus_tag="PLB01_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ78901.1"
                     /translation="MDYRYGELPLDLLQSLLSYEELLKLFLDLVSRLGGDVEEAMAWM
                     QELQRRGIIGKNVDLEAFRQALEQQGIVGRDGEGNLQLLGPGERRIRRNALEEIFSSL
                     QKAGPGLHSIPRAGGGSETLPETRPYEFGDDPARLDGIRTVANAARRDPDDLALLPAD
                     FEVFDVEAQSACATAIAIDVSHSMILYGEDRFTPAKKVALALTELILQKYPKDTIDVV
                     LFGDEARSVPLEELSKAQVGPFHTNTKDGLSLSRRLLMRRRSRNRQVFMITDGKPSCI
                     KEGGKLYKNPFGLDLKIVNRTLEEAEKLRRDGIQLTTFMIATDEALVDFVDTMTKIAR
                     GRAYYASPTDLATFVFRDYIRNRKKLFR"
     gene            2161..2673
                     /locus_tag="PLB01_16235"
     CDS             2161..2673
                     /locus_tag="PLB01_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78902.1"
                     /translation="MALWIAVGVGIFLLATGAFVLATRLVARRKGKIFLEGLGGVVRI
                     GTPCLLASAPLALLRNSESPGGAAGAAPLARGRALIPGTVALTPAGLAWEGLWGRTGS
                     YSFEEIQRLETDERLSSGRGFLRSKVLRVTATSGEVAEFVVSPGHAWEWRQALGEWAA
                     NRGRIAGTRI"
     gene            2717..3877
                     /locus_tag="PLB01_16240"
     CDS             2717..3877
                     /locus_tag="PLB01_16240"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ78903.1"
                     /translation="MASTLYPRRLTPPKQSFFLLGPRGTGKSTWVKSAFPRALRFDLF
                     DEGLYQSLLADPALFAGRLRTARPGAWIWVDEVQRLPSLLNEVQRHIEDHRLKFVLSG
                     SSARKLRRAGTNLLAGRALGKNMFPLLPSELGRDFDLEAVLSHGSVPIVWSSEDREAT
                     LRAYVQTYLKEEIQAEALARNLPGFARFLPVAALAHAQTVNVSSLARDCGVSRSTVDG
                     YLGILEDTLLAFRLPAFESRLRVRERRHPKLYWVDPGLARAARKGLGDVDAAERGALL
                     EGFVATCLRAHGEYDGLFDEVSYWAPADAVRTEVDFLLRRRKKYLAIEVKSSRRIPDD
                     GLRGLRAIAGLGGLVRRLLVLDSKDRIRTEDGIDVLPLREFFASLADGSLWP"
     gene            complement(3887..4741)
                     /locus_tag="PLB01_16245"
     CDS             complement(3887..4741)
                     /locus_tag="PLB01_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ78904.1"
                     /translation="MTVDARARRIEGTFAGLPLRGMSVAGRETWFHFPTLGFAFDLGR
                     SPWELVSVPHVFLSHAHLDHGAGLAYWFSQRRLLRLPGGTVHTHPAAVEAWRTIVALH
                     AGLEDVTYDARLEAMAPGSRVALRRDLSMACFSADHRVPTLAFVASEERHRLLPEFGS
                     KTREEIKILAAEGRNVAGRVAIPLAAYSGDTAAGIFEAAPPEFFRAKVLLLECSFVEE
                     RDKGRAAPFKHLHLDDIAERADLFENEVVLLTHVTLRTSASEIRRLVKKRLPARLAGR
                     ALPFLPEP"
     gene            4801..>5688
                     /locus_tag="PLB01_16250"
     CDS             4801..>5688
                     /locus_tag="PLB01_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HQQ78905.1"
                     /translation="MALFNHATKELTAKIVFYGPGLCGKTTNLKILHQRLERGAGKLL
                     SLSTAQDRTIYFDLLPIELGNIKGYTVRFQVCTVPGQVFYNETRRIVLRGVDGLVFVV
                     DSQWSMLSHNLESYQNLRDNLAQEKIPLESLPVVIQYNKRDLPSALSVEALQESLGFQ
                     SYPYVEAVASEGMGVLETFKLASKLTFVDLLRRLQRGWQPPAEADVPAAESSLGSASG
                     PGGSTQPFLSPGPAVSISAPAPEPDEQPAATPVLAFAGGSAAAEEEDADWVPPSAEGT
                     FADLSDDSQKSVASAARAEQ"
BASE COUNT          907 a         2026 c         1946 g          809 t
ORIGIN      
        1 cagttcgcgg ctggcgtagt agctgtcggg atcgttcggc tgcagcacgc gcagcgcggc
       61 cgcgtcgtcg tccctccgcc gccgcgccgc cgcgagcgcg agcggcagca gcgcgacgag
      121 gaggagcgtg aagaggccgg cgccgacgtc gcgatggtcg gtcgtcgaag gcacgaccgc
      181 agacgcgagg gccacgggga tcgagcccag ctcgtgcgcg agcggcccga acacctccac
      241 gaacgtgtgc cgggcgccgt gcgtgcggaa gaatcgccag aggagcgtca tgttccccgg
      301 atgctcgggc gtgccccgca actgctcgag aagcggcggc gtccagagag ccgccaggag
      361 aacgatcgac agggccagcg gtttcatcgc gacgaggcgc cggccgaggc gtcccgcgag
      421 ggcgaaggcc acggcaacga cgcacgcgac cgccggcgcc gtgccgagat gcgtctggac
      481 gaggaacgtc gcgacgaagg caacgcccgg cagccacgcg gcgccctgac gcgcgaggcc
      541 gagggcgaga acgacgagga gcgcgaaggg aacgaccacg gcgtgcggcc cccaggcgtg
      601 cgcgacccac ccggggccca tccgcgcgag gaggagcgcg agccccgccg caacgaggag
      661 cccgcgccgc aggccgagga tcctcgccgc ggaccaggcc gcggcgcacg cgaagagcgt
      721 cgtgaggaga agggcggcga gcgagaggcc cgcgctgcgc tgtcccgtgg ccgcgtagac
      781 cggggccatc aggtagaact ggacgggccc cgggtggctg aaaccgaagc gcgagtagac
      841 gcccgtgagc tgcttcccgt ggagcgcccg ccgctcggcc atctcgatga cggcgccgtc
      901 gtcgtcgacg ctcatcgggg gccacggctt caggatcgcg aaccaccacg acgggagcag
      961 cggcaggagc gcgaggagga cgggccagag cccggcgcgg cggtcgcgga cttccatgaa
     1021 gcggagtgac cataccccag tccatccccc gcgcggcgtc gtaccatgag ccgtggacta
     1081 ccggtacggc gaactgccgc tcgatctcct ccaatcgctc ctctcgtacg aagagctcct
     1141 gaagctcttc ctggacctcg tctcgcgcct cggcggggac gtggaggagg cgatggcgtg
     1201 gatgcaggag ctgcagcggc gcgggatcat cggcaagaac gtcgacctcg aggcgttccg
     1261 gcaggcgctc gagcagcagg gcatcgtggg gcgcgacggc gagggcaacc tccagctcct
     1321 cggccccggc gagcgccgca tccgccgcaa cgcgctggag gagatcttct cgtcgctcca
     1381 gaaggcaggg cccgggctcc actcgatccc gcgcgcgggc ggaggctccg agacgctgcc
     1441 cgagacgcgg ccgtacgagt tcggcgacga cccggcgcgc ctcgacggca tacggacggt
     1501 cgcgaacgcg gcgcgccgcg accccgacga cctcgcgctc ctccccgccg acttcgaggt
     1561 cttcgacgtc gaggcccaga gcgcgtgcgc gaccgcgatc gcgatcgacg tctcgcactc
     1621 gatgatcctc tacggcgagg accgcttcac gcccgccaag aaggtcgcgc tcgcgctcac
     1681 ggagctcatc ctccagaagt acccgaagga cacgatcgac gtcgtcctgt tcggcgacga
     1741 agcgcgctcc gtgccgctcg aggagctcag caaggcgcag gtcgggccgt tccacacgaa
     1801 cacgaaggac ggcctttcgc tctcgcgccg tctgctcatg cgccgccgct cgcgcaaccg
     1861 ccaggtcttc atgatcacgg acggcaagcc ctcgtgcatc aaggagggcg gcaagctcta
     1921 caagaacccg ttcgggctcg acctcaagat cgtgaaccgc acgctggagg aagccgagaa
     1981 gctccggcgc gacggcatcc agctcacgac gttcatgatc gcgaccgacg aggccctcgt
     2041 ggacttcgtc gacacgatga cgaagatcgc gcgcggccgg gcgtattacg cctcccccac
     2101 ggacctcgcg acgttcgtgt tccgggacta catccggaac aggaagaagc tcttccggta
     2161 atggccctct ggattgcggt cggagtcggg atcttcttgc tggcaaccgg tgcgttcgtt
     2221 ctcgcgacaa gactggttgc cagacggaaa gggaagattt tcttagaagg tttagggggc
     2281 gtcgttcgta tcggaacgcc gtgccttctg gcatccgccc ctcttgccct tctaagaaat
     2341 tccgaatccc cgggcggcgc cgcaggcgcc gcccccctag cgcgcggccg cgcgctcatc
     2401 cccggcaccg tcgccctcac gcccgccggc ctcgcgtggg aaggcctgtg gggtcgcacg
     2461 ggctcctact cattcgagga aattcagcgt ctcgagacgg acgagcgcct gtcgagcggg
     2521 cgcgggttcc tccgcagcaa ggtcctgcgc gtcaccgcga cgtccggcga ggtcgccgag
     2581 ttcgtcgtct cccccggcca cgcgtgggag tggcggcagg cgctcggcga atgggcggcg
     2641 aatcgcgggc ggatcgccgg aacacgaata tagatattgc ataccatgcc gtcattttgc
     2701 ggcgtaaaat gacggcgtgg ctagcacctt gtacccgcgg cggctgaccc cgccgaaaca
     2761 gtccttcttc cttctcgggc cgcgcggaac cggaaagagc acctgggtga agtcggcgtt
     2821 tccgcgcgcg ctccgcttcg accttttcga cgaagggctc taccagtcgc tcctcgccga
     2881 tccggccctc ttcgccgggc gtcttcgcac ggcgcggccg ggagcgtgga tctgggtgga
     2941 cgaggtccag cgcctgccct cccttctcaa cgaagtccag cgtcacatcg aagaccaccg
     3001 gctgaaattc gtcctgtccg gatccagcgc ccggaaattg cgccgcgcgg gcacgaatct
     3061 cctcgcgggc cgcgctctcg ggaagaacat gttcccgctc cttccttccg agctcggccg
     3121 cgacttcgac ctcgaggccg tcctgtccca cgggagcgtc ccgatcgtct ggtcgtcgga
     3181 agaccgcgag gcgacgctgc gcgcttacgt ccagacttac ctgaaggaag agatccaagc
     3241 ggaggccctg gcgcgcaacc tgccggggtt cgcgaggttc ctaccggtgg cggcgctcgc
     3301 tcacgcccag accgtcaacg tctcgagcct cgcccgggac tgcggcgtct cgcgatcgac
     3361 ggtcgacggg tacctcggca tcctcgaaga cacgcttctc gcgttccggc tgcccgcttt
     3421 cgagtcgcgc ctgcgcgtgc gcgagcgccg gcatccgaag ctctactggg tagaccctgg
     3481 actggcccgg gccgcgcgca aggggctcgg ggacgtggac gccgcagaac gcggcgctct
     3541 tctcgagggc ttcgtcgcga cctgcctcag ggcgcacggc gagtacgacg gcctcttcga
     3601 cgaagtctct tactgggcgc ccgccgacgc cgtccggacg gaggtcgact tcctgcttcg
     3661 gcgcaggaag aaatacctcg cgatcgaggt gaagtcttcg cgtcgcattc ccgacgacgg
     3721 ccttcggggg ctgcgcgcga tcgccgggct cggaggcctc gtccggcggc tcctcgtcct
     3781 cgactcgaag gaccggatcc ggaccgaaga cggaatcgac gtcctcccgc tccgggagtt
     3841 tttcgcctcc ctcgccgacg gatccctctg gccctgaaga tcccgcttac ggctccggca
     3901 ggaacggcag cgccctgccc gcgagccgcg cgggcaggcg ctttttcacg aggcggcgga
     3961 tctccgaggc cgacgtccgg agcgtcacgt gcgtcaggag cacgacctcg ttctcgaaga
     4021 ggtccgcgcg ctcggcgatg tcgtccaggt ggaggtgctt gaacggcgcc gcgcggccct
     4081 tgtcgcgctc ctccacgaac gagcactcga ggaggagcac cttcgcccgg aagaactcgg
     4141 gcggcgccgc ctcgaagatg ccggccgccg tgtcgcccga gtaggccgcg agcgggatcg
     4201 cgacgcgccc cgcgacgttc ctcccttccg ccgcgaggat cttgatctcc tcgcgcgtct
     4261 tcgacccgaa ctccggcagc agccggtggc gctcctcgga ggccacgaac gcgagcgtcg
     4321 ggacgcggtg gtcggccgaa aagcacgcca tcgagaggtc gcgccggagc gcgacgcgcg
     4381 agcccggcgc catggcctcg aggcgcgcgt cgtacgtcac gtcctcgagc ccggcgtgca
     4441 gggcgacgat cgtccgccag gcctccacgg cggcggggtg cgtgtggacc gtcccgcccg
     4501 gcaggcgcag cagccggcgc tgggagaacc agtacgcgag gccggccccg tggtcgaggt
     4561 gcgcgtgcga caggaagacg tgcgggacgg aaacgagctc ccacggagag cgtccgaggt
     4621 cgaacgcgaa gccgagcgtc gggaagtgga accacgtctc gcgacccgcg acggacatcc
     4681 cgcgcagcgg caggcccgcg aacgtcccct cgatccgccg ggcccgggcg tccaccgtca
     4741 cgggtcgagg atacccggcg cgtatactcg gacggcgtct caagcggtcc cggaggcacc
     4801 atggccctct tcaaccacgc caccaaggag ctgacggcca agatcgtctt ctacgggccc
     4861 gggctctgcg gcaaaacgac gaacctcaag atcctgcacc agcggctcga gcgcggcgcg
     4921 ggcaagctcc tgtccctttc gacggcacag gaccggacca tttacttcga cctcctcccg
     4981 atcgagctcg ggaacatcaa gggctacacg gtgcgcttcc aggtgtgcac cgttcccgga
     5041 caggtcttct acaacgagac gcgccggatc gtcctgcgcg gcgtggacgg gctcgtcttc
     5101 gtcgtggact cccagtggtc gatgctctcg cacaacctcg agagctacca gaacctgcgc
     5161 gacaacctcg cacaggagaa gatcccgctc gagtcgctgc ccgtcgtgat ccagtacaac
     5221 aagcgcgacc tcccgagcgc gctctcggtg gaagccctcc aggagtcgct cgggttccag
     5281 tcgtatccgt acgtcgaggc cgtcgcctcc gagggcatgg gcgtcctcga gacgttcaag
     5341 ctcgcctcca agctcacgtt cgtcgacctc ctgcggcgcc tccagcgcgg ctggcagccg
     5401 cccgccgagg cggacgtgcc ggccgccgag tcgtcgctcg ggtcggcgtc gggccccgga
     5461 ggctcgacgc agccgttcct gagccccggg cccgcggtgt cgatctcggc cccggccccc
     5521 gagcccgacg agcagcctgc cgccacgccc gtcctcgcgt ttgcgggcgg gtccgccgcc
     5581 gctgaagaag aagatgccga ctgggtcccg ccctcggccg aaggcacgtt cgcggacctc
     5641 tcggacgact cgcagaagtc cgtcgcttcg gcggcgcgcg ccgagcag
//
LOCUS       DAOMFY010000262         5665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_4887_length_5665_cov_5.488948, whole genome shotgun sequence.
ACCESSION   DAOMFY010000262 DAOMFY010000000
VERSION     DAOMFY010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_5665_cov_5.488948"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLB01_16255"
     CDS             <1..1078
                     /locus_tag="PLB01_16255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HQQ78906.1"
                     /translation="VEVRDADGGYVGRGFASPASPIAARLWTFEDRPLDAAFVAGRLD
                     AAVALRAAVLPPDTDGHRVVNAEGDFLPGLVVDRYGEVLSVQATTEGTERARDLWLPA
                     LADRFPGMTIVQRNDLPSRKGEGLPLEDAVLAGGPVAARAAFRERGLSFVAELAGGQK
                     TGFFLDQRENRHVVRGLAAGRRVLNLFSYSGAFGVAALAGGASRVVHVDVSQPALDLA
                     RENHRANGQRVDEPGAPAGTVCADVFEDLRSRVAAGETWDLVVTDPPAFAKRRSDVDR
                     ACRGYKDVNRLAFKLLAPGGVLLACSCSGAMSADLFQKVLFSAALDAGREARLLEKRG
                     AGPDHPVSIDCPEGEYLKAFLMAC"
     gene            1111..2832
                     /locus_tag="PLB01_16260"
     CDS             1111..2832
                     /locus_tag="PLB01_16260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ78907.1"
                     /translation="MRTRRTLEATLLSSLLSFPGVLLAQAPAAVPHVAGSVETSILEL
                     EIVVTDRDGRRVTGLQAADFEVRIGGKATAIASFFERRPTPVAAPPGAEAAVPAAAPD
                     AAPTAPAASRPPRHVVLFVDRLQLIEKWKADATFDGLRAVLRQTVVAPGDDAMIVTWN
                     RSVGSVVPFTSDVAAIERVLAREEKLCRRPLETDSVRRQLEEEAAWFRSLPTSSVSSG
                     VEVSQRAAAAEAYAQMRAKSNALKAVFSTLGGLPGRKIVVHASHRFSDLAGLEFFLTG
                     PVMYGSIPGDAREFNAHGIVESVAEAANANGVTLYTMFPPGWPDEAAVVRADASSTSN
                     PKINGPVEGARGDMIVMNESAALEPVALKTGGAYALGYKEARELAPRIAADLDSAYSI
                     GVEAPPGREGRPLAVAVRTKDRALKVRARRTVVSKTPEERVRDRVLANLFRPERERRL
                     PVALESSKAETTKGKTTVSLTLSVPVARLARTPSPKGESGAFSVFVASASSDGSFSEV
                     TRQRQPFDVPRADAAHADGGHFTYEVPVVVASDEARISVGVWDEVGREAGFLVVDVAG
                     GKATLRR"
     gene            2840..4531
                     /locus_tag="PLB01_16265"
     CDS             2840..4531
                     /locus_tag="PLB01_16265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ78908.1"
                     /translation="MKKKPVLLALALLLPRAGRAENPPDPPRLSGAVETSVLELDAVV
                     TDKDGRPVSGLKASDFAVRVGGKTVPIENFYERRTLPAGAAEPGTPAPAEPAALLAPT
                     AAGLRRPPRHLVLFLDRLYLWDKWKSDAAFDALRDILHRMVAEPGDDAMIVTWDRAVG
                     ALVPFTSDLASLERLLEKERIRSRQPRPEETTIRLLEDEARWFATNPVSSGTEPRTGT
                     GTGMATGMGTGTGGVEVSQRAAQAEAYAAMRSKAAALKAVCDAVGGLPGRKVLVHASH
                     RFSRIAGLEFSAGGLDAQEFNAMALVESVAEAANANGVTMYTFFPEGWNEDAMPLAPG
                     LSDAEVHNEGAALEVLADRTGGTFSLGWREGPALAARVVEDVESSYSLGVAASGRKPG
                     ASQAVKVTVRNPSLTVRARRTALERTPEERVEARVLSNLFRPEPPSRLRLSLDSASVE
                     AAKKKATATLHLRVPIAGLVKTPTARGASGAFSVFVVSAGADGSFSEVVRQRRPFEIP
                     KADADRASAGHFTYEVPVVVGAGEARISVGVWDEVGKDAGFLLVEVRDGQASVRR"
     gene            4540..>5665
                     /locus_tag="PLB01_16270"
     CDS             4540..>5665
                     /locus_tag="PLB01_16270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ78909.1"
                     /translation="MPPAAPRVALLGPFPPWRSGIADQDARLLAAMRRLGVDPLVLGF
                     SRMYPRFLYPGTLEYSEEEKKKILLEGEEGRTLDGLNPLSFVQAARLLSFEEISLLIL
                     PWWTSYFAPHVSLLLGTLRAESPSTVRLLLCHNVFDHEAHPLKDALTRAVLRRADRFA
                     VQNARSAREVASERPDAPIAVVPHPAEPRAVLPDRDAARGQLGVPLDAPVFLFSGLLR
                     PYKGWDVLLDAFAALRREVPRALLVLAGEPWGEARRLEAWAPAGVRLELRYLPERERG
                     LWFAAANAVVCPYRHATGSGIAADALAYGRPVIGSRVEGLEDVVEDGHSGLLVPPGDA
                     AALARAMELFVRENLGPRLAAGAARLRASFGPDDHARRVLA"
BASE COUNT          713 a         2044 c         2078 g          830 t
ORIGIN      
        1 cgtggaggtg cgggacgcgg acggcgggta cgtgggccgc gggttcgcgt cgccggcgtc
       61 gccgatcgcg gcgcgcctct ggacgttcga ggaccgcccg ctggacgcgg cgttcgtcgc
      121 cggccggctc gacgcggccg tcgcccttcg cgcggccgtg ctccctcccg acacggacgg
      181 ccaccgcgtc gtgaacgcgg agggagactt tctcccgggc ctcgtcgtgg accggtatgg
      241 agaggtcctg tccgtgcagg cgacgacgga gggcaccgag cgcgcgcggg acctctggct
      301 gcccgcgctc gccgatcggt tccccggcat gacgatcgtc cagcgcaacg accttccctc
      361 ccgcaaggga gagggtctgc cgctcgaaga cgccgttctc gcgggagggc cggtggccgc
      421 gcgcgccgcg ttccgcgagc gcgggttgag cttcgtcgcg gagctcgcgg gcggccagaa
      481 gacgggcttc tttctcgacc agcgcgagaa ccggcacgtc gtgcgcggcc tcgccgcggg
      541 gcggcgcgtc ctgaacctct tttcgtactc cggcgcgttc ggcgtcgcgg cgctcgcagg
      601 cggcgcgtcc cgcgtcgtcc acgtggacgt ctcgcagccg gcgctcgacc tcgcgcgcga
      661 gaaccatcgc gcgaacggcc agcgcgtcga cgagccgggc gcgccggccg ggaccgtctg
      721 cgcggacgtg ttcgaggacc tgcgctcccg cgtggccgcg ggagagacgt gggacctcgt
      781 cgtcacggac ccgcccgcgt tcgcgaagcg ccggagcgac gtggaccgcg cgtgccgcgg
      841 ctacaaggac gtgaaccgcc tcgcgttcaa gctcctcgcg ccggggggcg tcctcctcgc
      901 gtgctcgtgc tcgggggcca tgagcgcgga tctcttccag aaggtcctgt tctcggcggc
      961 gctcgacgcg ggccgcgagg cgcgcctcct cgagaagcgc ggcgcggggc ccgatcaccc
     1021 cgtgtcgatc gactgccccg agggcgagta cctcaaggcg tttctcatgg cctgctgagg
     1081 gcgtggcacg ctctctgctg gagcgtcgca atgcgaacca ggcgcactct cgaagcgacg
     1141 cttctttcgt cacttctctc gttccccgga gtcctgctcg cccaggcgcc ggcggccgtt
     1201 ccgcacgtcg cgggatcggt cgagacgtcg atcctcgagc tcgagatcgt cgtgaccgac
     1261 agggacggga ggcgcgtcac cggactccag gccgccgatt tcgaggtccg gatcggcggt
     1321 aaggccaccg cgatcgcgag tttcttcgag cgcaggccta cgccggtcgc ggcgccgcct
     1381 ggagccgagg ccgcagtccc cgccgcggcg ccggacgccg ccccgacggc tccggcggca
     1441 tcccgcccgc cgcggcacgt cgtcctcttc gtggatcgcc tccagctcat cgaaaagtgg
     1501 aaggcggatg cgacgttcga cgggttgcgg gcggtcctgc gccagacggt cgtcgcgccg
     1561 ggcgacgacg cgatgatcgt cacgtggaac cggtcggtag ggtcggtggt gccgttcacg
     1621 tcggacgtcg ccgcgatcga gagagtcctc gcgcgggagg agaagctctg ccgtcgcccg
     1681 ctggaaaccg attccgtccg ccgccagctc gaggaggagg ccgcctggtt ccggtccctc
     1741 ccgacgagct cggtttcgag cggcgtcgag gtgtcgcagc gggcggccgc cgcggaggcg
     1801 tacgcccaga tgcgcgcaaa gtcgaacgcg ctgaaggccg tgttctccac gctgggcggc
     1861 ctgcccggtc ggaagatcgt cgtccacgcg agccaccgct tctccgacct cgccggcctc
     1921 gagttcttcc tcacgggacc ggtgatgtac ggctcgatcc ccggggacgc ccgcgagttc
     1981 aacgcccacg gcatcgtcga gtccgtcgcg gaggcggcga acgcgaacgg cgtcacgctc
     2041 tacacgatgt tcccgcccgg ctggcccgac gaggcggcgg tggtgcgcgc agacgcgtcg
     2101 tcgacctcga acccgaagat caacgggccg gtcgaaggcg cgcgcggaga catgatcgtc
     2161 atgaacgaat ctgcggcgct cgaaccggtc gcgctgaaga cgggcggcgc gtacgccctc
     2221 ggctacaagg aggcccgtga gctcgcgccg cgcatcgcgg ccgacctcga ctcggcgtac
     2281 tccatcggcg tcgaggcgcc gcccggcagg gaggggagac cgctcgccgt cgccgtccgg
     2341 acgaaggacc gggccctgaa agtccgcgcg cggcggacgg tggtctcgaa gacgcccgaa
     2401 gagcgggtgc gcgatcgcgt cctcgcgaac ctcttccggc cggagagaga gaggcgcctg
     2461 cccgtcgccc tcgagtcgtc gaaggcggag acgaccaagg ggaagacgac cgtctccctg
     2521 acgctgtccg tgccggtcgc gcgcctcgcg cgcacgccct cgccgaaggg ggagagcggc
     2581 gcgttctccg tgttcgtcgc gtccgcctcc tcggacggga gcttctcgga agtcacgcgg
     2641 cagcggcagc cgttcgacgt cccgcgcgcg gacgcggcgc acgcggacgg cggccacttc
     2701 acgtacgagg ttccggtcgt cgtcgcgtcg gacgaggcgc gcatctccgt cggcgtgtgg
     2761 gacgaggtcg gccgggaggc cggcttcctc gtggtggacg tcgcgggcgg caaggcgacg
     2821 ctgcgccgat gatcgtcaca tgaagaagaa gccggtcctg ctcgcccttg cgctgctcct
     2881 gccgagggct ggccgggccg agaatccgcc ggacccgccg cgcctttccg gtgccgtcga
     2941 gacgtcggtc ctcgagctgg acgccgtcgt gacggacaag gacgggcggc ccgtttccgg
     3001 cctcaaggcc tcggacttcg cggtcagggt cggcggcaag accgtcccga tcgagaactt
     3061 ctacgagcgc cgcaccctcc cggccggtgc cgcggaaccc ggcacgcccg cacccgcgga
     3121 gccggccgcg ctgctggcgc cgaccgcggc cgggttgcgg cgcccgccgc ggcacctcgt
     3181 cctcttcctg gaccgcctct acctgtggga caagtggaag tcggacgccg cgttcgacgc
     3241 gctccgcgac atcctccatc ggatggtcgc cgagcccggg gacgacgcga tgatcgtcac
     3301 gtgggaccgc gcggtcggcg ccctcgtccc gttcacgtcg gacctcgctt ccctggaacg
     3361 ccttctcgag aaggagcgga tacggagccg ccagccccgt cccgaggaga cgacgatccg
     3421 cctgctcgaa gacgaggcca ggtggttcgc gaccaacccc gtgtcctcgg ggacggagcc
     3481 gaggacgggg acggggacgg ggatggcgac ggggatgggg acggggacgg gcggcgtcga
     3541 ggtgagccag cgcgccgccc aggccgaagc gtacgccgcc atgcggtcga aggccgcggc
     3601 gctgaaggcc gtctgcgacg cggtcggcgg gctgccgggc cgcaaggtcc tcgttcacgc
     3661 gagccatcgc ttctcgagga tcgcggggct cgagttctcg gccggcgggc tcgacgcgca
     3721 ggagttcaac gcgatggcgt tggtggagtc ggtcgcggag gcggcgaacg cgaacggcgt
     3781 cacgatgtac accttcttcc cggagggctg gaatgaggac gcgatgccgc tcgcgccggg
     3841 cctctcggac gccgaggtcc acaacgaggg cgcggcgctc gaggtcctcg ccgaccggac
     3901 cggcgggacg ttctcgctcg gctggcgcga gggcccggcc cttgcggcgc gcgtcgtcga
     3961 ggacgtcgag tcgtcgtact cgctgggcgt cgccgcgagc gggcgcaagc ccggtgcgtc
     4021 gcaggccgtg aaggtcacgg ttcgcaatcc gtcgctcacg gtgcgggcgc gccggaccgc
     4081 gctcgagcgc acgcccgagg agcgcgtcga ggcccgcgtc ctctcgaacc tgttccggcc
     4141 ggaacccccg tcgcggctgc gcctgtcgct ggactccgcg tccgtcgagg ccgcgaagaa
     4201 gaaggcgacg gcgacgctcc atctgcgcgt gccgatcgcc ggcctcgtga agacgccgac
     4261 ggcgcgcggc gcgagcggcg cgttctcggt cttcgtggtg tcggcgggcg ccgacggttc
     4321 gttctcggag gtcgtgcggc agcgccgccc gttcgagatc ccgaaggcgg acgccgatcg
     4381 cgcctccgcg ggccacttca cgtacgaggt ccccgtcgtc gtgggcgccg gggaggcgcg
     4441 catctcggtc ggcgtgtggg acgaggtcgg gaaggacgcc ggctttctcc tcgtcgaggt
     4501 ccgggacggc caggcctcgg tccgccgata atcgcgggca tgccgcccgc cgcgccgcgc
     4561 gtcgccctcc tcgggccgtt tcctccgtgg cgcagcggca tcgccgacca ggacgcgcgt
     4621 cttctcgccg cgatgcgccg gctcggcgtc gacccgctcg tcctcggctt ctcgcggatg
     4681 tatccgcgtt ttctttaccc cgggacgctg gagtattcgg aggaagagaa gaagaagatt
     4741 ctcttagaag gtgaagaggg gagaaccctc gacggactga atcccctgtc tttcgtgcag
     4801 gccgcacgtc ttctttcatt cgaagaaatc tccctcctga tcctcccctg gtggacgtcg
     4861 tatttcgcgc cgcacgtctc gctcctgctc gggacgcttc gcgccgaatc cccctccacg
     4921 gtccgcctgc ttctctgtca caacgtgttc gaccacgaag cgcatcccct gaaggacgcg
     4981 ctcacgcgcg ccgtgctccg ccgcgcggac cgtttcgcgg tccagaacgc gcgcagcgcg
     5041 cgcgaggtcg cctcggagcg tcccgacgcg ccgatcgcgg tggtgccgca cccggccgag
     5101 ccgcgcgccg tcctgccgga ccgcgatgcg gcgcgcgggc agctcggcgt cccgctcgac
     5161 gcgcccgtgt tcctcttcag cggtctcctc aggccctaca agggctggga cgtcctcctc
     5221 gacgcgttcg cggcgctgcg ccgtgaggtg ccgcgtgcgc tcctcgtcct cgcgggcgag
     5281 ccgtgggggg aggcgcgccg gctcgaggcg tgggcgcccg ccggagtccg cctcgagctg
     5341 cggtacctcc ccgagaggga acgcggcctc tggttcgccg ccgctaacgc cgtcgtctgt
     5401 ccgtaccggc acgcgacggg ttccggaatc gcggccgacg cgctcgcata cgggcggccc
     5461 gtgatcgggt cgcgcgtcga gggtctcgag gacgtcgtcg aggacgggca cagcgggctc
     5521 ctcgtcccgc ccggcgacgc ggccgccctt gcgcgcgcga tggagctctt cgtccgcgag
     5581 aacctcgggc cgcgcctcgc cgccggcgcc gcgcggctcc gggcctcgtt cggacccgac
     5641 gaccacgcgc gccgcgtgct cgcgc
//
LOCUS       DAOMFY010000263         5585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5000_length_5585_cov_6.818987, whole genome shotgun sequence.
ACCESSION   DAOMFY010000263 DAOMFY010000000
VERSION     DAOMFY010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5000_length_5585_cov_6.818987"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PLB01_16275"
     CDS             <1..1327
                     /locus_tag="PLB01_16275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ78910.1"
                     /translation="ALGELPAAARVRTTRLSRQEFTQRPTAAVARLRRAGFRTLAPLT
                     VSGRLLALFAVADRGGRVPLSAEDTELLETVLAPAALALDHARLFEELRAQADRYRNL
                     QEFHENVVAGSAAAIAATNGEGRFTSVNPAFAALVGRTAASLLGCRAAEILPPALSSA
                     NPPARLEADLGAGPRVLNVAVSPFPGAPAGSSARVLVLVDATETARLEKALADRERLT
                     ALGTLSAGVAHEVNTPLAGVAGFARLLLDETPGDDPRRPLVEKIERQAFRASRLVGSL
                     LDLARGRPREIEPLDPSDVAREAARALEDEGAARGASVRLEIAGRPPAVAGHRDALVQ
                     VLVNLVKNGIEAATAPGDLRPAAPEVVLAVSSGDGSVRFDVTDNGPGLASTDAARVFE
                     PFYSTKTAQGGTGLGLSMARDIIRAHGGTLTAASGSSGARFTVTLPAVT"
     gene            1331..2728
                     /locus_tag="PLB01_16280"
     CDS             1331..2728
                     /locus_tag="PLB01_16280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ78911.1"
                     /translation="MATVLILDDEPVIREVLGSVLAKAGHATREAATAAEGLQRLEKE
                     PVDLLLLDLMLPDRPGLEVLVEVRARHPEIPVVVVTAYSSVESAITAMREGAFHYIPK
                     PFRNEEVVHVVRQALEKRRLVAENRALRAKLDAPGGFRELVGRSAAMERVFETVRQAA
                     ASRSTILVTGESGTGKELVAKALHRLSPRSAGPFVTVHSGALPPDLLESNLFGHVRGS
                     FTGAVADKKGLFKAADGGTIFFDEIGTVPIDTQAKLLRVLQEREFTPVGAVEPIHSDV
                     RVIAATNADLTRAVAEGRFREDLFYRLCVITVNLPPLRERKDDIPLLVETLLARAAAE
                     NGRAVPSVTPAAMKALLDHDWPGNVRELENVLERALVLGHGVIGLDQLPDPVRRSVPR
                     AAAFPAEGLNFKDAVAAYERALLAAALARANGVQKRAAELLGLKPTTLNEMLKRHGML
                     PRDAKEPETEPASRA"
     gene            complement(2715..4367)
                     /locus_tag="PLB01_16285"
     CDS             complement(2715..4367)
                     /locus_tag="PLB01_16285"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQQ78912.1"
                     /translation="MLILNVRVPGEPARRIRLDRPVLTLGRSSTNDVPLADRTLSRVH
                     ARLEVSDANVRLVDLGSRNGTSLNGGRIGDPAVLAAGDRIQLGETLIDVLEESTTRVV
                     IEGLDETSKKTTFLQSSKDLLRPHRQTWDAKLGAEELARLNASLRMLNEISVELLGDI
                     PLQRLLELILEKTFTFLQPDRGLLMLADESGELKAEKVKYAPGVDPSDIRLSKTLIAS
                     VVDKKNGILLIDAATDAGLGAAESIRIQGITSCMAAPLFVEDKVIGLIYLEVRLGRKS
                     FSEEDLRLLTSLANTSAIKIQNLRLQEGAAAQQRIEREMALAWDIQRRMLPEAEPVLP
                     NTEILGRTIPSRTVSGDYYDFYERGDRTLDLVVADVCGKGMGASLLAASVQSAFQVWA
                     GESFPPDKLCSRLNDLVYRRTSPEKFVTFILALYDPASGAVVWTNAGHNPGILVRTDG
                     TVEMLGAHGPPLGLFPGKTYGSGTFTMAPGDLLALYTDGVTEAANAEDEEFGTDRLVA
                     ALRDLRGKPLPELERELAATLLAFTGGTPFGDDRTLVLLRRG"
     gene            4481..4723
                     /gene="rpmE"
                     /locus_tag="PLB01_16290"
     CDS             4481..4723
                     /gene="rpmE"
                     /locus_tag="PLB01_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQQ78913.1"
                     /translation="MKEKIHPEYHEVDVHCACGTTWKTRSTKNELRLEICSNCHPFFT
                     GKSKLVDTAGRVERFQKRYANVAKPAPKAKAPETPA"
     gene            4765..>5585
                     /locus_tag="PLB01_16295"
     CDS             4765..>5585
                     /locus_tag="PLB01_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HQQ78914.1"
                     /translation="MFQKLDDIERRYADLEARAASPEVLADHALATKTARSMREIAPV
                     VEKNRERKRIEAELAGARELLDTLPPADELRPVAQAEFEALSARRAAVDEELRLLLLP
                     KDPNDSKNVVLEIRAGTGGDEAALFAEELFRMYVRYAEGRGWKVEVIDRSDSGGVGGL
                     KDVQAIVEGEGAYSRLKYEGGVHRVQRVPATEAAGRIHTSAATVAVLPEAEDVDVVVN
                     DKDVRVDLFCASGPGGQGVNTTYSAVRLTHIPTGVVVQCQDERSQIKNKAKAMRVL"
BASE COUNT          798 a         2066 c         1964 g          757 t
ORIGIN      
        1 cgcgctcggc gagctgcccg ccgcggcgcg cgtccggacg acgcgcctct cgcgccagga
       61 gttcacgcag cgcccgaccg cggccgtcgc ccgcctccgg cgcgcaggat tccggacgct
      121 cgctcccctc acagtcagcg gccgcctgct cgcgctcttc gccgtcgccg accggggcgg
      181 ccgcgtcccg ctctcggcgg aagacaccga gcttctcgaa accgtgctcg cgcccgccgc
      241 gctcgcgctc gaccacgcgc ggctcttcga ggagctccgg gcgcaggcgg accgataccg
      301 caacctccag gagttccacg agaacgtcgt ggccggctcc gccgccgcga tcgcggcgac
      361 gaacggcgag ggacgcttca cgtcggtcaa cccggccttc gccgcgctcg tcggccggac
      421 cgccgcgtcg ctcctgggct gccgcgccgc cgagatcctt ccacccgcgc tctcctccgc
      481 gaacccgccc gcgcgcctcg aggccgacct cggagccggc ccgcgcgtcc tgaacgtcgc
      541 cgtctcgccc ttcccgggcg cgcccgcggg gtcgagcgcc cgcgtcctcg tcctcgtcga
      601 cgcgacggag accgcccggc tcgagaaggc gctcgcggac cgcgagcgcc tcaccgccct
      661 cggaacgctc tcggcgggcg tggcccacga ggtgaacacg ccgctggccg gcgtcgcggg
      721 tttcgcgcgc ctccttctcg acgagacgcc gggcgacgac ccgcgccggc ccctcgtcga
      781 gaagatcgag cggcaggctt tccgggcttc gcgcctcgtc ggaagcctcc tcgacctcgc
      841 gcgggggcgg ccgcgcgaga tcgagcccct cgacccttcc gacgtcgccc gcgaggccgc
      901 ccgcgcgctc gaggacgagg gcgccgcacg cggggcctcc gtgcggctcg agatcgccgg
      961 acggccgccg gccgtcgcgg ggcaccggga cgccctcgtg caggtcctcg tgaacctcgt
     1021 caagaacggg atcgaggccg ccacggcgcc cggcgacctg cgtccggccg cccccgaggt
     1081 cgtcctcgcc gtgtcgtccg gcgacgggag cgtgcgcttc gacgtcacgg acaacgggcc
     1141 cggcctcgcg tccacggacg ccgcgcgcgt cttcgagccc ttctactcga cgaagacggc
     1201 gcagggcggg accggactgg gcctctcgat ggcgcgggat atcattcgcg cgcacggcgg
     1261 cacgttgacc gcggcgtccg ggtcgtccgg cgcgcgcttc acggtcacgc ttcccgccgt
     1321 gacctgacac atggccacgg tactcatcct cgacgacgag cccgtcatcc gcgaggtgct
     1381 cggctcggtg ctcgcgaagg cgggccacgc gacgcgcgag gccgccacgg ccgccgaagg
     1441 cctgcagcgc ctcgagaagg agccggtcga cctgctcctt ctcgacctca tgctcccgga
     1501 ccggccgggt ctcgaggtcc tcgtggaggt ccgcgcccgg caccccgaga tccccgtcgt
     1561 cgtcgtgacg gcctactcgt ccgtcgagag cgcgatcacg gcgatgcgcg agggcgcctt
     1621 ccactacatc ccgaagccgt tccggaacga ggaagtcgtc cacgtcgtcc ggcaggcgct
     1681 cgagaagcgg cgcctcgtcg ccgagaaccg cgcgctccgc gccaagctcg acgcgcccgg
     1741 cggattccgc gagctcgtgg gccggtccgc tgcgatggag cgcgtcttcg agaccgtgcg
     1801 ccaggcggcg gcctcgcgct cgacgatcct cgtcacgggc gagagcggca cgggcaagga
     1861 gctcgtcgcg aaggcgctgc accgcctgtc gccgcgctcc gcgggcccgt tcgtgaccgt
     1921 ccactcgggc gccttgccgc ccgaccttct cgagtcgaac ctcttcgggc acgtccgcgg
     1981 ctcgttcacg ggcgccgtcg cggacaagaa ggggctcttc aaggccgccg acggcggcac
     2041 gatcttcttc gacgagatcg gcaccgttcc gatcgacacg caggccaagc tcctgcgcgt
     2101 cctccaggag cgcgagttca cccccgtggg cgccgtcgag ccgatccact ccgacgtgcg
     2161 cgtgatcgcc gcgacgaacg cggacctcac gagggccgtc gccgaaggcc gcttccgcga
     2221 ggacctcttc taccggctct gcgtcatcac cgtgaacctg ccgccgctgc gcgagcgcaa
     2281 ggacgacatc ccgctcctcg tggagacgct cctcgcgagg gccgccgccg agaacgggcg
     2341 cgccgtgccg tccgtgacgc ccgcggcgat gaaggcgctc ctcgaccacg actggccggg
     2401 caacgtccgc gagctcgaga acgttctcga gcgcgcgctc gttctcgggc acggcgtgat
     2461 cggcctcgac cagctccccg acccggtccg gcgctccgtc ccgcgcgcgg ccgcgttccc
     2521 cgcggagggc ctgaacttca aggatgcggt cgccgcttac gagcgcgcgc ttctcgcggc
     2581 cgccctcgcg cgtgcgaacg gcgtccagaa gcgcgccgcc gagctcctcg gcctgaagcc
     2641 gacgacgctc aacgagatgc tgaagcggca cgggatgctc ccgcgcgacg cgaaggagcc
     2701 ggagaccgag cccgctagcc gcgcctgaga aggacgagcg tccggtcgtc cccgaacggc
     2761 gtcccgcccg tgaacgcaag gagcgtcgcg gcgagctcgc gctcgagttc cggcagcggc
     2821 ttgccccgca ggtcccggag cgccgcgacg agccggtccg tcccgaactc ctcgtcctcg
     2881 gcgttcgcgg cctccgtcac cccgtccgtg tagagcgcga gaaggtcgcc cggtgccatc
     2941 gtgaacgtgc cggacccgta ggtcttcccg gggaagaggc cgagcggcgg gccgtgggcg
     3001 ccgagcatct cgaccgtccc gtccgtcctc acgaggatgc cggggttgtg ccccgcgttc
     3061 gtccagacga ccgccccgga cgccgggtcg tagagcgcga ggatgaacgt gacgaatttc
     3121 tcggggctcg tccggcggta gacgaggtcg ttcaggcgcg agcagagctt gtcgggcggg
     3181 aagctctcgc ccgcccagac ctggaacgcg gactggacgg aggccgcgag gagcgacgct
     3241 cccatccctt tgccgcagac gtccgcgacg acgaggtcga gcgtccggtc gccgcgctcg
     3301 tagaagtcgt agtagtcgcc cgacacggtg cgcgacggga tcgtgcgccc gaggatctcc
     3361 gtgttcggga gcacgggctc ggcctcgggg agcatgcgcc gctggatgtc ccacgcgagc
     3421 gccatctcgc gctcgatgcg ctgctgcgcg gccgcgcctt cctgcaggcg cagattctgg
     3481 atcttgatcg cggacgtgtt cgcgagcgac gtgaggagac ggagatcctc ctcgctgaag
     3541 ctcttccggc cgagccgcac ctcgaggtag atgaggccga tgaccttgtc ctcgacgaag
     3601 agcggcgccg ccatgcagga cgtgatcccc tggatgcgga tggactcggc cgcgccgagg
     3661 ccggcgtccg tcgcggcgtc gatgaggagg atgccgttct tcttgtccac gaccgacgcg
     3721 atcagggtct tggacaggcg gatgtcggac ggatcgacgc cgggcgcgta cttgaccttc
     3781 tcggccttca gctcgcccga ctcgtccgcg agcatcagga gaccgcggtc gggctgcagg
     3841 aacgtgaacg tcttctcgag aatgagctcg aggagcctct ggagagggat gtccccgagg
     3901 agctccacgg agatctcgtt gagcatccgg agcgacgcgt tgaggcgcgc gagttcctcg
     3961 gcgccgagct tcgcgtccca ggtctgccga tgggggcgga gcaggtcctt ggaagactga
     4021 aggaacgtcg tcttcttcga ggtctcgtcg agtccctcga tcacgacgcg cgtcgtggac
     4081 tcctcgagga cgtcgatgag cgtctcgccg agctgtatcc ggtcgcccgc ggcgaggacg
     4141 gcggggtcgc cgatgcgccc gccgttcagc gacgtcccgt tccgcgaccc gaggtcgacg
     4201 agccggacgt tggcgtcgga cacctcgagc cgggcgtgga cgcgggagag cgtgcgatcc
     4261 gcgagcggga cgtcgttcgt ggacgagcgg ccgagcgtga ggacggggcg gtcgagacgg
     4321 atccgccggg cgggctcccc cggcaccctg acattcagga tgagcacggc gcgagtgtaa
     4381 ccggaccggg gccgccgcgg gacggggcgc gtgcgggcgt gctatcctca cgggttccgg
     4441 accggctcac catgcggccg gccgacgaaa gggttccccc atgaaggaaa agatccaccc
     4501 cgagtaccac gaggtcgacg tccactgcgc gtgcgggacc acgtggaaga cgaggagcac
     4561 caagaacgag ctccggctcg agatctgctc gaactgccac ccgttcttca cgggcaagag
     4621 caagctcgtc gacaccgcgg gccgcgtcga gcgcttccag aagcgctacg cgaacgttgc
     4681 gaagcccgcc cccaaggcca aggctcccga aaccccggcc tgagccggtg accggcgcgg
     4741 cccgaagggc cgctcccacc cgccatgttc cagaagctcg acgacatcga acggcggtac
     4801 gcggacctcg aggcccgcgc cgcctcgccc gaggtccttg cggaccacgc cctcgcgacg
     4861 aagaccgccc ggtcgatgcg ggagatcgcg cccgtcgtcg agaagaaccg cgagcgcaag
     4921 cgcatcgaag ccgagctcgc gggcgcacgc gagctcctcg acacgcttcc gccggccgac
     4981 gagctgcgcc ccgtggccca ggccgagttc gaggcgctga gcgcgcggcg ggcggccgtc
     5041 gacgaggagc tccgcctcct cctcctcccc aaggacccga acgattcgaa gaacgtcgtc
     5101 ctcgagatcc gcgcgggcac gggcggcgac gaggcggcgc tcttcgccga ggagctgttc
     5161 cggatgtacg tgcgctacgc cgagggccgc ggctggaagg tcgaggtgat cgaccggagc
     5221 gactccggcg gcgtgggcgg cctcaaggac gtccaggcga tcgtcgaagg cgagggcgcg
     5281 tactcgcgcc tgaagtacga gggcggcgtc caccgggtcc agcgcgtccc cgcgaccgaa
     5341 gccgccgggc gcatccacac gtcggcggcg accgtcgcgg ttttgcccga ggccgaggac
     5401 gtggacgtcg tcgtgaacga caaggacgtg cgcgtggacc tcttctgcgc atccggcccg
     5461 ggagggcagg gcgtcaacac gacctattcc gccgtgcgcc tcacgcacat cccgacgggc
     5521 gtcgtcgtcc agtgccagga cgagcgctcg cagatcaaga acaaggcgaa ggcgatgcgc
     5581 gtcct
//
LOCUS       DAOMFY010000264         5581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5010_length_5581_cov_9.195621, whole genome shotgun sequence.
ACCESSION   DAOMFY010000264 DAOMFY010000000
VERSION     DAOMFY010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5010_length_5581_cov_9.195621"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLB01_16300"
     CDS             <1..345
                     /locus_tag="PLB01_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQQ78915.1"
                     /translation="ISMEIKGRDLVEGVPKTLTLTDAEVREALAETVGIILDAVRVAL
                     EKTPPELSADIMDKGIVLTGGGSMLKNLDRRLREETGLPIATAEEPLSSVALGTGAML
                     ADVDLLRKISLD"
     gene            352..1212
                     /gene="mreC"
                     /locus_tag="PLB01_16305"
     CDS             352..1212
                     /gene="mreC"
                     /locus_tag="PLB01_16305"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQQ78916.1"
                     /translation="MASPSLAARRPEILLAVLLSLCIVGLSLQVRRPGGRTAGQAWLL
                     AAVSPFVEGVTALRAGVADVGTWASSRRRLLGANRALSDEIERLEGELLKLRDAERDK
                     TRLLELFGTQPSPPAGTVPARLIAVESSGPFRTGLLDRGSESGVRVDGVVVAPGGLVG
                     RVIAIGPRTARLQLLSDKTAAVGVLLPRGARAGVAKGDGKGGIGVEYVPTIEPVEKND
                     LVVSSGTDGVYPNGLPVGRVSATTRKSTLFWDIQLEPATDPRRESMVFVLPPVKRADV
                     QGGPVPGERR"
     gene            1209..1712
                     /gene="mreD"
                     /locus_tag="PLB01_16310"
     CDS             1209..1712
                     /gene="mreD"
                     /locus_tag="PLB01_16310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQQ78917.1"
                     /translation="MKTLRVAIGLALAILATLVLGALRFPTPPDLFLLVVAYMARGGA
                     VVPAMLAGLPAGLIEDALQGPQGLLGLHAFSKILTGYLLATIGARTIVEKPLAVGGLL
                     AGAVLAEALTITFLLWVLRGELLAPAPWLLLGRAATTGLLGGALHAASRVPWRQRREA
                     RRRMRLN"
     gene            1712..3595
                     /gene="mrdA"
                     /locus_tag="PLB01_16315"
     CDS             1712..3595
                     /gene="mrdA"
                     /locus_tag="PLB01_16315"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQQ78918.1"
                     /translation="MPASAREDRRPVLTRIDLAMRILWALLGLIAGVYWVHQVLRGED
                     YERQAENNRLRKVPVTAPRGFILDRNGVVLAENEPSFTLLLYRRETKDLDASIRFVAG
                     LFNRPFDEMKRRVERDRSYYDFVPVVLQENLTVSEVGAVQARALEHPELVVQKTERRV
                     YTQGTVAAHLLGQLGEATAEQVAARAKARPGDEALPRPGEAIGQQGVEASYQDLLAGV
                     SGSRSYVIDSFGREVGESGHVDPMPGNTLQLTIDAGLQHLAEDYFAGKVGAAVALDPK
                     TGEVLALVSAPAYDPNLFTRRVTRAEWDEILTDPKRPLNNRVLQNLYSPGSTWKPFMA
                     YAILKHGIDPKERVFCPGGLTFYGRPFRCHGTHGSVDLATALQVSCDSYFYTMGKRLG
                     IDAIADAGKHFGFGRLSGIDLAHEKAGIVPSSEWSAAVRKHPWYAGETISVSIGQGPV
                     LVSALQQARALCGIANADGALPTPHLFHIGENVRTGERFVYRPAVKESVSWAGLPRDV
                     VLDGLWRVVNQPGGTAYGSRVAGIDMCGKTGSVQVVGQKEAKKGHLLPEQLRDHGWFI
                     GFAPKDDAQIVVAVFAEHGEHGASAAAPLAAKMCAYWVTKRRAGPPPAVAQAGSAASR
                     GAN"
     gene            3596..4696
                     /gene="rodA"
                     /locus_tag="PLB01_16320"
     CDS             3596..4696
                     /gene="rodA"
                     /locus_tag="PLB01_16320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQQ78919.1"
                     /translation="MSRLAEILPHRIDLRLLFLAILLTAAGVVMVVSTTAGSPRADLA
                     GKQVLFAVLGLIAAGVFLAVDYRILLQYAPGLYGLTLLVLLYLPIFGKRIAGAKSWIK
                     IGGGFQLQPSEFARISIALLLAWILENDDRPILRTRTIVLLTAAVAAPVLLVLAQPDL
                     GVALTYVPFLVAALFFGGLKGRWWAALLVAGALAVGGSWFLLKDYQKDRIRTFLDPNL
                     AARGAGYQVRQARIAIGSGGILGKGWKQGTQSRLGFLPVRHTDFIFAALAEEWGFAGV
                     AVLLGAYGFLIARALAIARDARDRGGSMLVLLLIVNIAGQFLVNVAMNVGILPTTGIT
                     LPFLSYGGSSLIATWCMVGLILSVAYRRYVNV"
     gene            4722..>5581
                     /locus_tag="PLB01_16325"
     CDS             4722..>5581
                     /locus_tag="PLB01_16325"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQQ78920.1"
                     /translation="MLKELLVSASDRETRISVLEEGRVAEVQIERRKQRGTVGNIYKG
                     RVTRVLPGMQSAFVDVGLEKDAFLYVADVGEEVDDFDAFGTGGAAEAISAVAASVAAL
                     PHDAAASPASETGSAVPDESLPKPVLPSIDELLREGQELVVQVTKDQIAHKGVRVTTH
                     VTLPGRTLVYMPTIDHVGVSRRIENEEERARLKDILTALKKGPGGYIVRTAGEGRAAE
                     EFAADRRYLTKLWEQIRKKGERSGAPTLLHRDLDLTLRAARDVFGSDFATLWVDDDEE
                     YQRIVEFLDQV"
BASE COUNT          752 a         2071 c         1918 g          840 t
ORIGIN      
        1 atctcgatgg agatcaaggg ccgcgacctc gtcgagggcg tgccgaagac gctcacgctg
       61 acggacgcgg aagtccgcga ggcgctcgcc gagaccgtcg ggatcatcct cgacgcggtc
      121 cgcgtcgccc tcgagaagac gccgcccgag ctctcggccg acatcatgga caagggcatc
      181 gtcctcacgg gcggcggctc gatgctgaag aacctcgacc ggcgcctgcg ggaggaaacg
      241 gggctcccga tcgccacggc ggaggagccg ctctcttccg tcgccctcgg gacgggcgcg
      301 atgctcgcgg acgtcgacct cctgcggaag atcagcctcg actagacgcg cgtggcttcg
      361 ccgtcactcg ccgcccgccg acccgagatc ctgctggcgg tgctgctctc gctgtgcatc
      421 gtcggactgt ctctccaggt gagacggccc ggcgggcgga ccgccggcca ggcgtggctt
      481 ctcgccgcgg tgtccccgtt cgtcgagggc gtgacggctc tgcgcgcggg ggtcgccgac
      541 gtcggaacat gggcctcgtc gcggcggcgt cttctcggcg cgaatcgcgc cctctcggac
      601 gagatcgagc gcctcgaggg cgagctcctg aagcttcgcg acgccgagcg cgacaagaca
      661 cgcctgctcg agctcttcgg cacgcagccg tcgccgccgg cgggcaccgt acccgcccgc
      721 ctcatcgcgg tcgaatcctc cggcccgttc cgcacgggcc tcctcgaccg cggctccgaa
      781 agcggcgtcc gcgtcgacgg cgtcgtcgtc gcgccgggag gcctcgtggg ccgcgtgatc
      841 gcgatcggac cgcgcaccgc ccgcctgcag ctgctctccg acaagaccgc cgccgtgggc
      901 gtcctccttc cgcgcggggc gcgggcgggc gtcgccaagg gggacggaaa gggcgggatc
      961 ggcgtcgagt atgtccccac gatcgagccc gtcgagaaga acgatctcgt cgtctcctcg
     1021 ggaacggacg gcgtctaccc gaacggcctt cccgtcggcc gcgtttcggc gacgacgcgc
     1081 aagagcacgc tcttctggga catccagctc gagccggcga ccgacccgcg gcgggagtcg
     1141 atggtgttcg tcctcccgcc cgtcaagcgc gcggacgtac agggcggtcc cgtgcccgga
     1201 gagcgccggt gaagaccctt cgcgtcgcaa tcggcctcgc cctcgcgatc ctcgcgacgc
     1261 tcgtgctcgg cgcccttcgc ttccccacgc ctcccgatct cttcctcctc gtcgtggcgt
     1321 acatggcgcg cggcggcgcc gtcgtgcctg cgatgctcgc gggcctgccc gcgggcctca
     1381 tcgaggacgc actccagggg ccgcagggac tcctcgggct gcacgccttc tcgaagatcc
     1441 tgacgggcta tctcctcgcg acgatcggag cgcggacgat cgtcgagaag cccctcgcgg
     1501 tcggcgggct gctcgcgggc gccgtcctgg ccgaggccct cacgatcacg tttctcctct
     1561 gggtcctgcg cggcgagctt ctcgcccctg cgccgtggct cctcctcggc cgcgcggcga
     1621 cgaccggcct gctcgggggc gcccttcacg cggcctcgcg cgttccgtgg cgccagcgcc
     1681 gcgaagcgcg gcgccggatg cggttgaact gatgccggcg agcgcccggg aagaccgccg
     1741 ccccgtcctc acgcgcatcg acctcgcgat gcggatcctg tgggctctcc tcggcctcat
     1801 cgcgggcgtc tactgggtcc accaggtcct gcgcggcgag gactacgagc gccaggccga
     1861 gaacaaccgg ctccggaagg tccccgtcac ggccccgcgc ggcttcatcc tggaccgcaa
     1921 cggcgtcgtc ctcgccgaga acgagccctc cttcacgctt ctcctctacc ggcgggagac
     1981 gaaggacctg gacgcctcga tccggtttgt cgccgggctc ttcaaccggc ctttcgacga
     2041 gatgaagagg cgcgtcgagc gagaccgctc gtactacgac ttcgtgcccg tcgtcctcca
     2101 ggagaacctg accgtctcgg aggtcggcgc cgtgcaggcg cgcgcgctcg agcaccccga
     2161 gctcgtcgtc cagaagacgg agcgccgcgt ctacacgcag gggaccgtcg ccgcgcacct
     2221 tctcggccag ctcggcgagg cgacggccga gcaggtggcc gcgcgcgcga aggcgcgccc
     2281 gggagacgag gcgctgcccc gcccgggcga ggcgatcggg cagcagggcg tcgaggcctc
     2341 gtatcaggat ctcctcgcgg gcgtctcggg ctcgcgctcg tacgtcatcg actcgttcgg
     2401 ccgcgaggtc ggcgagtccg gtcacgtcga ccccatgccc ggcaacacgc tccagctcac
     2461 gatcgacgcc gggctccagc acctcgcgga ggactacttc gccggaaagg tcggagcggc
     2521 cgtcgcgctc gacccgaaga cgggcgaagt cctcgcgctc gtctcggcgc ccgcgtacga
     2581 cccgaacctc ttcacgcggc gcgtgacgcg cgccgagtgg gacgagatcc tgacggaccc
     2641 gaagcgcccc ctgaacaacc gcgtccttca gaacctctac tcgccgggct cgacgtggaa
     2701 gccgttcatg gcgtacgcga tcctcaagca cgggatcgac ccgaaggagc gcgtgttctg
     2761 tccgggcggg ctcacgttct acggccgccc gttccgctgc cacgggacgc acgggagtgt
     2821 cgacctcgcg accgcgctcc aggtctcctg cgactcgtac ttctacacga tgggcaagcg
     2881 cctcggcatc gacgcgatcg cggacgcggg gaagcacttc ggcttcggcc gcctcagcgg
     2941 gatcgacctc gcgcacgaga aggccggcat cgtcccgtcg tcggaatgga gcgccgcggt
     3001 gcgcaagcat ccctggtacg cgggcgagac gatctccgtt tcgatcggcc agggaccggt
     3061 cctcgtctcg gcactccagc aggcccgcgc gctgtgcggg atcgcgaacg cggacggcgc
     3121 gcttccgacg ccgcacctct ttcacatcgg cgaaaacgtc cgcaccggag agcgcttcgt
     3181 ctaccggccc gccgtgaagg agtccgtctc gtgggccggc ctcccgcgcg atgtcgtcct
     3241 cgacgggctc tggcgcgtcg tgaaccagcc gggcgggacc gcgtacggct cgcgcgtcgc
     3301 cggcatcgac atgtgcggca agacgggctc cgtccaggtc gtcggccaga aggaggcgaa
     3361 gaagggccac ctccttccgg agcagctgcg cgaccacggg tggttcatcg gcttcgcgcc
     3421 gaaggacgac gcgcagatcg tcgtcgcggt cttcgccgag cacggcgagc acggggcgag
     3481 cgccgcggca cctctcgccg cgaagatgtg cgcctactgg gtcacgaagc gccgggccgg
     3541 cccgccgccg gccgtcgcgc aggcgggaag cgcggcctcg cgaggggcga actgagtgtc
     3601 gcggctcgcc gagatcctcc cccaccggat cgacctgcgg ctgctcttcc tcgcgatcct
     3661 cctcaccgcg gccggcgtcg tcatggtcgt ctcgacgacc gccggctcgc cgcgcgccga
     3721 cctcgcgggc aagcaggtcc tgttcgccgt cctcggcctg atcgcggcgg gcgtcttcct
     3781 cgccgtcgac taccggatcc tcctccagta cgcgccgggg ctctacggcc tcacgctcct
     3841 cgtgctcctg tacctgccga tcttcgggaa gcgcatcgcg ggggcgaagt cgtggatcaa
     3901 gatcggcggc ggctttcagc tccagccgtc cgagttcgcc cggatctcga tcgcgctcct
     3961 cctcgcctgg atcctcgaga acgacgaccg gccgatcctc cggacgcgga cgatcgtcct
     4021 cctcaccgcg gccgtcgccg ccccggtcct tctcgtcctc gcccagcccg acctcggcgt
     4081 cgcgctgacg tacgtgccgt tcctcgtcgc cgcgctcttc ttcggggggc tgaagggacg
     4141 ctggtgggcc gcgctcctcg tcgcgggagc gctcgcggtc ggcggctcgt ggttcctcct
     4201 gaaggactac cagaaggacc gcatccggac gttcctcgac ccgaacctcg cggcgcgcgg
     4261 cgccggctac caggtgcggc aggcccggat cgcgatcggc tcgggcggga tcctcgggaa
     4321 gggctggaag cagggcacgc agagccggct cgggttcctg ccggtgcgcc acacggactt
     4381 catcttcgcg gcgctcgcgg aggaatgggg gttcgcgggc gtcgcggtcc tcctcggcgc
     4441 gtacgggttc ctgatcgcgc gggcgctcgc catcgcacgg gacgcgcgcg accgcggcgg
     4501 gtcgatgctc gtcctgctcc tcatcgtgaa catcgcgggg cagttcctcg tgaacgtcgc
     4561 gatgaacgtc ggcatcctcc cgacgacggg catcacgctg ccgttcctgt cgtacggcgg
     4621 ctcgtcgctc atcgcgacgt ggtgcatggt cggcctcatc ctttccgtcg cgtaccggcg
     4681 gtacgtgaac gtctgacggc cctcgcgcgt acactgcgcc ggtgctgaag gaactcctcg
     4741 tctcggcctc cgaccgcgaa acgcgcatct ccgtcctcga ggaggggcgc gtggccgagg
     4801 tgcagatcga gcgccggaag cagcgcggga ccgtcgggaa catctacaag gggcgcgtga
     4861 cgcgcgtcct gcccgggatg cagtccgcgt tcgtggacgt cggcctcgag aaggacgcgt
     4921 tcctctacgt cgcagacgtc ggcgaggagg tcgacgactt cgacgccttc gggacgggcg
     4981 gcgccgccga ggcgatctcg gcggtggcgg cgagcgtcgc cgcccttccc cacgacgccg
     5041 ccgcctcgcc cgcgtccgag accggctcgg cggtgccgga cgagagcctc ccgaagcccg
     5101 ttctcccgtc gatcgacgag ctgctccgcg agggccagga gctcgtcgtc caggtcacga
     5161 aggaccagat cgcccacaag ggcgttcgcg tcacgacgca cgtgaccctc ccgggccgga
     5221 cgctcgtcta catgccgacg atcgaccacg tcggcgtctc gcggcgcatc gagaacgagg
     5281 aggagcgcgc ccgtctcaag gacatcctga ccgcgctcaa gaagggcccg ggcggctaca
     5341 tcgtgcgcac ggccggcgag ggccgcgccg ccgaggagtt cgccgccgac cggcgctacc
     5401 tcacgaagct ctgggagcag atccgcaaga agggcgagcg ctccggcgcg ccgacgctcc
     5461 tgcaccgcga cctcgatctc acccttcgcg ccgcccgcga cgtcttcggc tcggacttcg
     5521 cgaccctctg ggtggacgac gacgaggagt accagcggat cgtggagttc ctcgaccagg
     5581 t
//
LOCUS       DAOMFY010000265         5531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5071_length_5531_cov_7.868700, whole genome shotgun sequence.
ACCESSION   DAOMFY010000265 DAOMFY010000000
VERSION     DAOMFY010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5071_length_5531_cov_7.868700"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..922)
                     /locus_tag="PLB01_16330"
     CDS             complement(41..922)
                     /locus_tag="PLB01_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQQ78921.1"
                     /translation="MSAAPPALPGYVPGDYKTDLKPVWCPGCGDFGVLTALYRAMAEL
                     QLTPELTAVVSGIGCSSRLPGYVETYGFNTVHGRALPLATGVAVSRPDVKVIAVGGDG
                     DGLAIGGNHFMHSARRNLDVTYILMDNEIYGLTKGQVAPTTPTGDKTKTTHYGNPEPS
                     VDPCELAISFGATWVGRAFSGDLKGTVDLIAKALQHRGFAFLNVMSPCVTWRGDDQFK
                     SLKAKAKPLPEGHDRSSRHEALKFTRETDVLSTGVLYEIKTPSLNDRLDTIKVTARAG
                     APMVTVPEIVRTFLPSF"
     gene            complement(919..2682)
                     /locus_tag="PLB01_16335"
     CDS             complement(919..2682)
                     /locus_tag="PLB01_16335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQQ78922.1"
                     /translation="MSRRDLTIAMVGAGGDGIVTMGDILSQAGARDGLNVIKTEAYGP
                     QIRGGESSCVVRMSPDAIYEQGDAVDVLVVFSWADFGRFKGEVQPAPNAVVLFDEADP
                     VARTREDLGLGADGHWLAVPFAKLAGDAGAKGSKNVVGLGVLTALFGLREETIRRAVV
                     KRFGRKKAAVGELALKAFEAGIAHGGTLPGLKDRGITFTPGPSKLLMSGNEASALGAI
                     HAGCRFFAGYPITPSTEVLQFAEEWLPKMGGSVIQTEDELSAIGAVVGASFAGVKSMT
                     ATSGPGLSLMTEMLGLASMAEVPCVVVDVQRGGPSTGLPTKSEQSDLWQAVFAGHGDA
                     PRVVIAASDVEDCFHTTVEAFNISEEFQIPVVVLTDQSIGQRRETLDPTRLVHAVVDR
                     AVPTPADLEDYKRYRDTPEGYSPMSVPGMKGGIYQTNGLEHDESGRPASMHLVHEKMN
                     AKRYRKLKPIRDKYQRARRFGPEKADLGIVCWGSSKGAVKEALLMAEARGEKVAAFIP
                     EMIFPFPVKALEEFLESVKQVLVIEVSFAAQFYKYLRTFTTLPEDTIIFKRSGGKNLT
                     ISEVDDQIRKFFSPDRKKGVA"
     gene            complement(2755..3528)
                     /locus_tag="PLB01_16340"
     CDS             complement(2755..3528)
                     /locus_tag="PLB01_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQQ78923.1"
                     /translation="MSAQKEKPRPHTPQAPPHAKGSSRGLPRHHHVETHFEGSETVRD
                     VVLGMSDGLTVPFALAAGLSGAVASSRVVLVAGVAELAAGAIAMGLGGYLAAKSEADT
                     WHSELRREHEEVVEVPAEEKEEVRKVFMGWGLEGNVLESATEAVCSDRDRWVDFMMRE
                     ELHLSEPDPKRARVSALTIGGSYVAGGAIPLGPYLFPVPITTALLASVVVTLIALGVF
                     GAFKGRLTGRPAAAEALRTVLVGALASGAAYALARLISG"
     gene            complement(3525..4235)
                     /locus_tag="PLB01_16345"
     CDS             complement(3525..4235)
                     /locus_tag="PLB01_16345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ78924.1"
                     /translation="MDLRDKVVLITGAGRGIGRAVALAFAFEGAKVALVGRTKKSLTE
                     VQKELKELGATSAVLPGDVSDEGDVARVVAATEQQLGTVDVLVNNAGVFATAPVDKMD
                     ALVFDHILAVNLRGPFLMARAVLPEMKARRRGHIVNVASTAGRRAFAGGGAYCASKFG
                     LAGLSEAMMYEARTSDVRVSVVYPSTVATDLVRKAGMAFDEKKAIQPEDVAAGIVALV
                     KLDDRALVKGLEIWQTNP"
     gene            complement(4280..5500)
                     /locus_tag="PLB01_16350"
     CDS             complement(4280..5500)
                     /locus_tag="PLB01_16350"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQQ78925.1"
                     /translation="MPLPARAADDAALAHAKDLLSHHILIDGHNDLPWAIREAKAAPG
                     DVAAYDLRTKTSGQTDIPRLKAGRVGGQFWSVYVPGEATEGWARMQLEQIELARRVVE
                     RYPDTFALALTADDAEKAFKAGKIASFIGMEGGHVLENSLGALRAYYALGARYMTVTH
                     NTSLAWADSATDPPARHGGLTKFGEEVVREMNRLGMLVDLSHAAPETMRAALRVSEAP
                     VIFSHSSARAICDVPRNVPDDVLRLLPKNGGVVMIAFVTGFVSPEAAKILMPAFQEFN
                     QRSKTLASEKERDALWKEILAPLKVPKATIGQVADHVEQVRRVAGADHVGLGADYDGN
                     DTWPIGLEDVSMYPNLFAELIRRGWSDADLVKLAGANVLRALRRAEGVAKRLQAERPP
                     STATLGSLDGPPAK"
BASE COUNT          796 a         1863 c         2016 g          856 t
ORIGIN      
        1 cgcgaagagc gcggcccgtc gagattccgg aacgtcccgg tcagaaggac ggcaggaacg
       61 tccggacgat ctcggggacc gtaaccatcg gtgcccccgc ccgggccgtc accttgatcg
      121 tgtcgagccg gtcgttcagc gaaggggtct tgatctcgta gagcactccg gtcgagagga
      181 cgtcggtctc acgcgtgaac ttgagagcct cgtgccgcga cgagcggtcg tgtccctcgg
      241 gcagcggctt cgccttggcc ttgagcgact tgaactggtc gtcgccgcgc cacgtgacgc
      301 agggggacat cacgttcagg aacgcgaatc cgcggtgctg gagcgccttg gcgatgagat
      361 ccacggtgcc cttgaggtcg ccggagaacg cgcggcccac ccacgtggcg ccgaaggaga
      421 tcgcgagctc gcacgggtcc acggacggct ccgggttgcc gtagtgcgtc gtcttcgtct
      481 tgtcgcccgt cggggtcgtg ggcgcgacct ggcccttcgt gaggccgtag atctcgttgt
      541 ccatgaggat gtaggtcacg tcgagattcc ggcgggccga gtgcatgaag tggttgccgc
      601 cgatcgcgag accgtcgccg tcgccgccga ccgcgatgac cttcacgtcg ggccgcgaga
      661 cggcgacgcc ggtagcgagc gggagggcgc gcccgtggac cgtgttgaag ccgtacgtct
      721 cgacgtatcc ggggaggcgc gaggagcagc cgatgccgga cacgacggcc gtgagctccg
      781 gcgtgagctg gagctcggcc atggcgcggt agagcgccgt caggacgccg aaatcgccgc
      841 agccggggca ccagacgggc ttgaggtcgg tcttgtagtc gccggggacg tagccgggga
      901 gggcgggcgg agcggcgctc atgcgacccc cttcttgcgg tcgggcgaga agaacttgcg
      961 gatctggtcg tcgacctcgg agatcgtgag gttcttgccg ccggacctct tgaagatgat
     1021 cgtgtcctcg ggcagcgtcg tgaacgtccg caggtacttg tagaactggg cggcgaagga
     1081 gacctcgatc acgaggacct gcttcacgct ctcgaggaac tcctcgagcg ccttcaccgg
     1141 gaacgggaag atcatctcgg ggatgaacgc ggcgactttc tcgccgcgcg cctcggccat
     1201 gaggagcgct tccttgacgg cgcccttcga cgagccccag cagacgatgc cgaggtcggc
     1261 cttctcggga ccgaagcggc gcgcgcgctg gtacttgtcg cggatgggct tgagcttgcg
     1321 gtaccgcttc gcgttcatct tctcgtgcac gaggtgcatc gaggccgggc ggcccgattc
     1381 gtcgtgctcg agaccgttcg tctggtagat gccgcctttc atgcccggga cgctcatcgg
     1441 cgaatacccc tcgggcgtgt cccggtagcg tttgtagtcc tcgaggtcgg ccggggtcgg
     1501 gacggcgcgg tcgacgaccg cgtggacgag ccgcgtcggg tcgagggtct cgcggcgctg
     1561 gccgatggac tggtccgtca ggacgacgac gggaatctgg aattcctcgg agatgttgaa
     1621 ggcctcgacg gtcgtgtgga agcagtcctc gacgtcggag gccgcgatga cgacgcgcgg
     1681 cgcgtcgccg tggcccgcaa agacggcctg ccagaggtcg gattgctcgc tcttcgtcgg
     1741 gaggcccgtc gaggggcctc cgcgctggac gtccacgacc acgcacggga cctcggccat
     1801 ggaggcgagg ccgagcatct cggtcatgag cgagagaccg gggccggacg tcgccgtcat
     1861 ggacttgacg cccgcgaagg acgcgccgac gacggcgccg atcgcggaga gctcgtcctc
     1921 ggtctggatg acggagccgc ccatcttcgg gagccattcc tcggcgaact gaaggacctc
     1981 ggtggagggc gtgatcgggt agccggcgaa gaagcggcac cccgcgtgga tcgcgccgag
     2041 cgccgaggct tcgttgccgg acatgaggag cttcgacggg ccgggcgtga aggtgatccc
     2101 gcggtccttg aggcccggga gcgtcccgcc gtgcgcgatc cccgcctcga acgccttgag
     2161 cgcgagctcg ccgacggccg ctttcttgcg tccgaagcgt ttcacgacgg cgcggcggat
     2221 cgtctcctcg cgaaggccga agagcgcggt cagcacgccg agcccgacga cgttcttcga
     2281 gcccttggcg cccgcgtcgc ccgcgagctt cgcgaacggc acggcgagcc agtgcccgtc
     2341 cgcgccgagg ccgaggtcct cccgggttcg cgccacgggg tccgcctcgt cgaagaggac
     2401 gaccgcgttc ggcgcgggct ggacctctcc cttgaagcgg ccgaagtcgg cccaggagaa
     2461 gacgacgagg acgtccacgg cgtccccctg ttcatagatc gcgtccggac tcatccgcac
     2521 cacgcacgag gactcgccgc cccggatctg gggtccgtac gcctcggtct tgatgacgtt
     2581 gagcccgtcg cgcgcgccgg cctgggacag gatgtcgccc atggtgacga tcccgtcgcc
     2641 gccggccccg accatcgcga tggtgagatc acgtctcgac atggcgcaca gggtgtccct
     2701 tacggctccc gccgtccatg gtgcaaacgg atcatgagcg ggcgagagag cgtctcagcc
     2761 tgagatgagg cgcgcgagcg cgtaggcggc gccggaggcg agcgccccga cgagcaccgt
     2821 ccggagggcc tcggcggcgg ccgggcggcc ggtgagacgc cccttgaacg cgccgaagac
     2881 gccgagcgcg atgagcgtca cgacgacgga cgcgaggagg gccgtcgtga tgggtacggg
     2941 gaacaggtag ggaccgagcg ggatcgcgcc gccggcgacg tacgagccgc cgatcgtgag
     3001 ggccgacacg cgcgcccgtt tcgggtcggg ctcggagagg tgcagctcct cgcgcatcat
     3061 gaagtccacc cagcggtccc ggtcggagca gacggcctcc gtcgcggact cgaggacgtt
     3121 tccctcgagg ccccagccca tgaagacctt ccggacctct tccttctcct ccgcggggac
     3181 ctcgacgacc tcctcgtgct cgcggcggag ctcggaatgc cacgtgtcgg cctcgctctt
     3241 ggccgcgagg tagccgccga ggcccatcgc gatggcgccg gccgcgagct ccgcgacgcc
     3301 cgcgacgagg acgacgcgcg agctggccac ggcgcccgag aggccggccg cgagcgcgaa
     3361 cgggaccgtg agcccgtcgg acatgccgag gacgacgtca cggacggtct cggagccctc
     3421 gaagtgcgtc tcgacgtggt ggtggcgggg gagaccccgc gagcttccct tcgcgtgcgg
     3481 cggcgcctgg ggcgtgtgcg gccggggctt ctccttctgc gcgctcacgg attcgtctgc
     3541 cagatctcga gtcccttcac gagggcgcgg tcgtccagct tcacgagcgc cacgattcct
     3601 gccgcgacgt cctccggctg gatcgccttt ttctcgtcga aggccatgcc cgccttcctc
     3661 acgaggtccg tcgccaccgt ggacgggtag acgacggaca cgcgcacatc cgacgtgcga
     3721 gcctcgtaca tcatggcttc cgagagcccc gcgaggccga acttcgacgc acagtacgcg
     3781 cccccgcctg cgaaggcgcg gcgcccggcc gtcgacgcga cgttcacgat gtgcccccgg
     3841 cggcgcgcct tcatttcggg caggaccgcc cgcgccatga ggaacggccc gcgcaggttc
     3901 acggccagga tgtggtcgaa gaccagcgcg tccatcttat cgaccggcgc cgtcgcgaag
     3961 acgccggcgt tgttcacgag gacgtcgacg gtgccgagct gctgctccgt ggccgcgacg
     4021 acgcgcgcga cgtcgccttc gtccgagacg tcgcccggca gaacggccga cgtggcgccg
     4081 agctccttga gctccttctg gacttccgtg aggctcttct tcgtgcggcc gacgagcgcc
     4141 accttcgcgc cttcgaaagc gaaggccagg gcgacggcgc ggccgatccc gcgcccggcg
     4201 cccgtgatga ggacgacctt gtctctcaag tccatgggtg tcctccccgg ggcgggattc
     4261 taggcccccg ggggcgaggt cacttcgcgg gcggtccgtc cagcgacccg agggtcgccg
     4321 tggagggcgg ccgttcggcc tggagccgct tcgcgacgcc ctcggcccgc cggagcgcgc
     4381 ggagcacgtt cgctcccgcg agcttgacga gatccgcgtc gctccagccg cgtcggatca
     4441 gctcggcgaa gaggttcggg tacatggaga cgtcctcgag cccgatgggc caggtgtcgt
     4501 tgccgtcgta gtcggcgccg agcccgacgt ggtcggctcc ggcgaccctc cggacttgct
     4561 cgacgtggtc ggcgacctgg ccgatcgtcg ccttcgggac cttcagcggt gcgaggatct
     4621 ccttccagag cgcgtcgcgc tccttctcgc tcgcgagcgt cttcgagcgc tggttgaact
     4681 cctggaaggc cggcatgagg atcttcgcgg cctcgggcga gacgaaaccc gtgacgaacg
     4741 cgatcatcac gacgccgccg ttcttcggga ggagccgaag gacgtcgtcg ggaacgttgc
     4801 gcgggacgtc gcagatcgcc cgcgccgagg agtgcgagaa gatcaccggc gcctcggaga
     4861 cgcgcagggc cgcgcgcatc gtctccggcg cggcgtgcga gaggtccacg agcattccaa
     4921 gccggttcat ctcgcggacg acctcctcgc cgaacttcgt gaggccgccg tggcgggccg
     4981 ggggatccgt ggcgctgtcg gcccaggcga gcgaggtgtt gtgcgtgacg gtcatgtagc
     5041 gtgcgccgag cgcgtagtag gcgcgcaggg ctccgagcga attctcgaga acgtggccgc
     5101 cctccatccc gatgaacgac gcgatcttcc cggccttgaa ggccttctcg gcgtcgtcgg
     5161 ccgtgagcgc gagggcgaac gtgtccgggt agcgctcgac gacgcgccgc gcaagctcga
     5221 tctgctcgag ctgcatgcgc gcccagccct ccgtcgcctc tcccgggacg tagacggacc
     5281 agaactgccc gccgacgcgc ccggccttga ggcgcgggat gtccgtctgg ccggaggtct
     5341 tcgtccggag gtcgtaagcg gcgacgtcac cgggcgccgc cttcgcctcg cggatcgccc
     5401 acggcaggtc gttgtggccg tcgatcagga tgtggtgcga gaggaggtcc ttcgcgtgcg
     5461 cgagcgccgc gtcgtcggcc gcgcgggcgg ggaggggcag cgcgagcgcg gcgccgagcg
     5521 cagcggcgag c
//
LOCUS       DAOMFY010000266         5501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5113_length_5501_cov_5.842820, whole genome shotgun sequence.
ACCESSION   DAOMFY010000266 DAOMFY010000000
VERSION     DAOMFY010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5113_length_5501_cov_5.842820"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLB01_16355"
     CDS             <1..489
                     /locus_tag="PLB01_16355"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ78926.1"
                     /translation="LPGVGPGATRDLPGSGGGQPSLAADPVAQRKLADLEAAAAKEPG
                     NYALLVQLGNTAYDVESWQKAVDAYERALKVQGGDPNVMTDLGVAYRNVGNGTKALEY
                     FGKALAKDPAHWPAAFNQAVVYGIDRGDKAKAKEILVKIKKEHPEVKSIDGLLASLDQ
                     KG"
     gene            489..1868
                     /locus_tag="PLB01_16360"
     CDS             489..1868
                     /locus_tag="PLB01_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQQ78927.1"
                     /translation="MAESPGDLFDDELPSPGTETASDAPLADRMRPRELSEVAGPPDL
                     VGESSFLARAIAADRVPSLVFWGPPGSGKTTLARIIAAKTKARFLAFSAVTSGIKEIK
                     TVMEEAARARRRGRRTLLFVDEIHRFNRAQQDAFLPFVEAGDIVLVGATTENPSFELN
                     GALLSRLRVVILPAIEEKDLVLLMKRALADPTRGLNALVTVDEDSFEWLGRFADGDAR
                     RALTALEAAVAQLLSEANSSSSSSPPVLSVPVLEDLFKRKVLLYDKSGEEHFNIISAL
                     HKSLRDSDVDASLYWLARMLEAGEDPLYVARRLVRFASEDVGLADPQALVLAVAAKDA
                     VHFIGMPEGALALSQIAVYLALAPKSNALYVAYGEAASDIREHPNLPPPLAIRNAPTK
                     LMKDAGYGTGYRYAHDLPEKTAGLSCLPDALQGRNYYRPDGEGREKALKERADALRAL
                     RARVGKKKE"
     gene            2029..3240
                     /locus_tag="PLB01_16365"
     CDS             2029..3240
                     /locus_tag="PLB01_16365"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQQ78928.1"
                     /translation="MPSEKIFSASSSPVPVSSPVGLWHAALVPTSEYPVYFDLRIAAG
                     KGGTLSGAIVNGGVETPLSSVTWDGTTLVLGIASYDMTITATRAGDALDGTYRRTVVS
                     GVAEVPFTASRAAPPLPPAPADGKSLAGSWAVEVGGPPGKVEKLTGVFAQKGAVLTGT
                     FLSVTGDYGALHGWFDGERMLLTVFDGVHVYRYDGELLPDGTLAGEHRSRTSPPVAWK
                     ASRLDPRAAASNLPGGFEVVRAKDPRAPYAFAYPDADGKIVSSADPRFAGKPMVVNFM
                     GTWCPNCADEAPVLGALAARFGPKGVGFVSLAFEYTDDVERNRRQVKRFVERFGVTYP
                     VLIAGTTRTARETAAVKPLEGFEGYPTTLFLDRAHRIVKIHSGFDGPATGERHARAKK
                     EFEVAVEALLR"
     gene            complement(3253..4002)
                     /locus_tag="PLB01_16370"
     CDS             complement(3253..4002)
                     /locus_tag="PLB01_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ78929.1"
                     /translation="MRFADHFSGHAADYARYRPAYPPALFEALAALVSRHTLCWDAGT
                     GSGQAACGLADHFAEVVATDASAEQIAHATPCAGVTYRVAPADQSGLDDATVDLVTVG
                     QAVHWFSLKSFYEEVRRVGAPGSVIAVWAYDLFTVSESAAVESVFRAFHSTVEAYWPP
                     ERALVARRYADLLFPFEEIPVTPVTMTAAWSLERVLGYLSTWSSVKAFTKATGEDPVA
                     ALSAELTAVWGDPADSKTIAWPLILRTGRIA"
     gene            4076..>5501
                     /locus_tag="PLB01_16375"
     CDS             4076..>5501
                     /locus_tag="PLB01_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQQ78930.1"
                     /translation="MNRELDLSRREVIGLGSAALAAAALPDVLFAAVPGTTEGIGYFA
                     RFGVTEKLIRDALAAALARGGDYADLYFEHSYYASLALEDGEVNRGSRYVQLGVGVRV
                     VKGDQTGYGFTEDLTPDSLLATAKTAAAIATGPARSAPLSLRVSKDVPNRYPVKRRWE
                     DVKTEERLPVLASLNQKAFALDPRIKKVNLNFSDEGSVVLLADSEGRLIEDVRPMTGL
                     YVGIVAEQDGRKETNGYNVSGRADLGYYSPDRLDRVVKEAVQRTTILFDAVQPAAGEM
                     PVVLAAGSSGILLHEAIGHGMEADFNRKGTSIYADKIGKPIAKSFVNIVDEGTQEAAR
                     GALNVDDEGTPVGRTNLVEGGVLTTYLHDTISAKHYGVKPTGNGRRESYQYAPMPRMR
                     STYMLPGPHKTEEIIASVKKGIYCTSFTNGQVNIGPGDFTFYVKNGFLIEDGKLTRPI
                     KDVNIIGNGPKVLEKVDMVSDDLKI"
BASE COUNT          903 a         1927 c         1878 g          793 t
ORIGIN      
        1 ctgcccggag tcggccccgg cgcgacgcgc gacctgcccg ggagcggcgg cggccagccc
       61 tccctcgcgg ccgatcccgt cgcgcagcgc aagctcgcgg acctcgaggc cgccgcggcg
      121 aaggagccgg gcaactacgc gctgctcgtc cagctcggca acacggcgta cgacgtcgag
      181 agctggcaga aagccgtcga cgcgtacgag cgcgcgctga aggtgcaggg cggcgacccg
      241 aacgtcatga ccgacctcgg cgtcgcgtac cgaaacgtcg ggaacggcac gaaggcgctg
      301 gagtacttcg ggaaggcgct cgcgaaggac ccggcccact ggcccgcggc gttcaaccag
      361 gcggtcgtct acggaatcga ccgcggcgac aaggcgaagg cgaaagagat cctcgtcaag
      421 atcaagaagg agcaccccga ggtcaagtcg atcgacgggc tcctcgcctc gctggaccag
      481 aaggggtaat ggccgagtct ccgggcgacc tgttcgacga cgagcttccg tcgcccggaa
      541 ccgaaaccgc ctccgacgcc ccgctcgcgg accgcatgcg tccgcgcgag ctctccgagg
      601 tcgccgggcc tcccgatctc gtgggcgagt cgtcgttcct cgcgcgcgcc atcgccgccg
      661 accgcgtccc gtcgctcgtg ttctggggcc ctccgggcag cgggaagacg acgctcgcac
      721 ggatcatcgc ggcgaaaacg aaggcgcgct tcctcgcgtt ctcggcggtc acctccggga
      781 tcaaggagat caagacggtg atggaggagg ccgcgcgcgc gcgccggcgc gggcggcgca
      841 cgctcctctt cgtggacgag atccaccgct tcaaccgcgc gcaacaggac gcgttcctgc
      901 cgttcgtgga ggcgggcgac atcgtcctcg tcggtgcgac gaccgagaac ccctccttcg
      961 agctgaacgg cgcgctcctc tcccggctgc gtgtcgtcat cctgccggcg atcgaagaga
     1021 aagacctcgt tctgctcatg aagagggcgc tggcggatcc cacgaggggc ctcaacgcgc
     1081 tggtgacggt ggatgaagat tccttcgaat ggttaggacg ctttgccgac ggcgatgccc
     1141 gaagggcgct cacggcattg gaggccgccg tcgcccagct cctttcagaa gcaaattctt
     1201 cctcctcctc ttctcctcca gttctctcgg tccccgtcct cgaggacctc ttcaagcgga
     1261 aggtgctgct ctacgacaag tccggtgaag agcacttcaa cataatctcg gcgctccaca
     1321 agtcgctccg cgattcggac gtcgacgcgt cgctctactg gctcgcgcgg atgctcgagg
     1381 cgggagagga cccgctctac gtcgcgcggc gcctcgtccg gttcgcgtcg gaggacgtgg
     1441 gcctcgcgga tccgcaggcg ctcgtcctcg cggtcgccgc gaaggacgcc gtccacttca
     1501 tcgggatgcc ggagggagcg ctcgcgcttt cgcagatcgc cgtgtacctc gctctcgccc
     1561 cgaagtcgaa cgcgctctac gtggcctacg gcgaggccgc gagcgacatc cgcgagcacc
     1621 cgaacctgcc gcccccgctc gcgatccgca acgcgccgac gaagctcatg aaggacgcgg
     1681 gctacggcac cggctaccgc tacgcgcacg acctgccgga gaagacggcg ggcctctcgt
     1741 gcctgccgga cgcgctccag gggcggaact actaccggcc cgacggcgaa gggcgcgaga
     1801 aggcgctcaa ggagcgcgcc gacgcgctgc gggcgctccg cgcccgcgtc ggaaagaaga
     1861 aagaataagt ctgcttcgga cccgcgcaga aatgcacgcg ccgcggcatc gggagacggt
     1921 ctgcaaagtt tctttgaagg gaataagggc cgggaacgcg cgcgttcacg aagcgtgcgc
     1981 acatccaccg cccgcgccgc gctgcttctg ctggccgttc tttccctatt gccttctgag
     2041 aaaatcttct ctgcctcctc ttcccccgtc cccgtctctt cgcccgtcgg cctctggcac
     2101 gcggctctgg tcccgacgtc cgagtacccc gtgtacttcg acctgcgaat cgcggcgggg
     2161 aagggcggga cgctctccgg cgcgatcgtg aacggaggcg tcgagacgcc gctgtcgtcc
     2221 gtgacgtggg acgggacgac gctcgtcctc gggatcgcga gctacgacat gacgatcacg
     2281 gccacgcgcg cgggcgacgc cctcgacggg acgtaccggc gcacggtcgt ttccggtgtc
     2341 gccgaggtgc cgttcacggc ttcgcgcgcg gcgccgccgc tgccgcccgc tcccgccgac
     2401 gggaagtcgc tcgccggctc atgggccgtc gaggtcggcg ggccacccgg caaggtcgag
     2461 aagctgaccg gcgtcttcgc gcagaagggc gccgttctga ccggcacgtt cctctcggtc
     2521 acgggcgact acggcgcgct ccacggctgg ttcgacggcg agcggatgct cctcacggtc
     2581 ttcgacggcg tccacgtcta ccgctacgac ggcgagctgc tccccgacgg gaccctcgcg
     2641 ggcgagcacc gctcgcgcac gtctccgccg gtggcgtgga aggcctcgcg cctcgacccg
     2701 cgggccgccg cgtccaacct cccgggcgga ttcgaggtcg tgcgcgcgaa ggacccgagg
     2761 gcgccgtacg ccttcgcgta ccccgacgcc gacgggaaga tcgtgtcctc ggcggacccg
     2821 cgcttcgcgg ggaagccgat ggtcgtgaat ttcatgggga cgtggtgtcc gaactgtgcc
     2881 gacgaggcgc cggtcctcgg ggcactcgcg gcgcgcttcg gcccgaaggg cgtcggcttc
     2941 gtctccctcg ccttcgaata cacggacgac gtagagcgga accggcgcca ggtgaagcgc
     3001 ttcgtcgagc gcttcggcgt gacgtacccg gtcctcatcg cggggacgac gcgcacggcc
     3061 cgggagacgg cggccgtgaa gccgctcgag ggcttcgagg gctacccgac gacgctcttc
     3121 ctggaccgcg cgcaccggat cgtgaagatc cactccggtt tcgacggccc cgcgaccggc
     3181 gagcggcacg cgagggccaa gaaggagttc gaggtggcgg tcgaggcgct gctgcgctga
     3241 acgaagggac cgtcaggcga tccggccggt gcggaggatc agcggccagg cgatcgtctt
     3301 cgagtccgcg ggatctcccc agacggccgt gagctccgcg gagagcgccg cgacggggtc
     3361 ctcgcccgtg gccttcgtga aggccttcac cgaggaccag gtcgacaggt agccgaggac
     3421 cctctccagg ctccacgcgg ccgtcatggt cacgggcgta acggggattt cttcgaaagg
     3481 gaagagaagg tcggcgtaac ggcgggcgac gagggcgcgt tccggcggcc aatacgcctc
     3541 gacggtcgaa tgaaaggcgc ggaagacgct ttcgacggcc gccgattccg agacggtgaa
     3601 gagatcgtag gcccataccg cgatgacgct gcccggtgcc ccgacccttc tcacttcttc
     3661 gtagaaactc ttcaatgaaa accagtgaac cgcctgtccc acggtcacga ggtcgacggt
     3721 tgcgtcgtcc agcccggatt ggtccgcggg cgcgacgcga tacgtcacgc ccgcgcacgg
     3781 cgtcgcatgc gcgatctgct cggcgctcgc gtccgtcgcg acgacctcgg cgaagtggtc
     3841 cgcgaggccg caggccgcct gcccgctccc ggttcccgcg tcccagcaca gggtgtggcg
     3901 ggacacgagc gccgcgaggg cttcgaagag cgccggcggg taggccggac ggtaccgggc
     3961 gtagtccgcg gcgtggcccg aaaagtggtc ggcgaagcgc aacctcggtc ctccccgaac
     4021 gtaatatcag aaaacgaacc gggcgcgagg tgtgcgccgc cctaccggag gctccatgaa
     4081 ccgtgaactc gacctctccc gccgcgaggt catcggtctc ggctccgccg ctctggccgc
     4141 cgcggccctc ccggacgtcc tcttcgccgc cgtgcccggc accaccgagg gcatcgggta
     4201 cttcgcgcgc ttcggcgtga cggagaagct catccgggac gcgctcgcgg ccgccctcgc
     4261 gcgcggcggc gactacgcgg acctctactt cgagcacagc tactacgcct cgctcgccct
     4321 cgaggacggc gaggtgaacc gcgggtcgag gtacgtccag ctcggcgtcg gcgtccgcgt
     4381 cgtgaagggg gaccagacgg gctacgggtt caccgaggac ctcacgcccg actcgctcct
     4441 cgcgaccgcg aagacggccg ccgcgatcgc gaccgggccc gcgcggagcg cgcccctctc
     4501 cctgcgcgtc tcgaaggacg tcccgaaccg ctatcccgtg aagcgccgct gggaggacgt
     4561 gaagacggag gagcgcctcc cggtcctcgc gtccctcaac cagaaggcct tcgcgctcga
     4621 cccgcggatc aagaaggtga acctcaactt cagcgacgag ggcagcgtcg tcctcctcgc
     4681 ggacagcgag ggccgcctca tcgaggacgt ccggccgatg acgggcctct acgtcgggat
     4741 cgtcgccgag caggacggac gcaaggagac gaacgggtac aacgtctccg ggcgcgctga
     4801 cctcggctac tacagcccgg accgcctcga ccgcgtcgtc aaggaggcgg tccagcgcac
     4861 gacgatcctc ttcgacgccg tccagccggc ggcgggggag atgccggtcg tcctcgcggc
     4921 cggctcctcc gggatcctcc tgcacgaggc gatcggccac gggatggagg cggacttcaa
     4981 ccgcaagggg acgtcgattt acgccgacaa gatcggcaag ccgatcgcga agagcttcgt
     5041 gaacatcgtg gacgagggga ctcaggaggc cgcgcgcggc gccctgaacg tggacgacga
     5101 gggaacgccc gtcggccgga cgaaccttgt cgagggcggc gtcctgacga cgtacctcca
     5161 cgacacgatc tcggcgaagc actacggcgt gaagccgacg gggaacggcc ggcgcgagag
     5221 ctaccagtat gccccgatgc cgcgcatgcg ttcgacgtac atgctcccgg gcccgcacaa
     5281 gaccgaggag atcatcgcct ccgtgaagaa ggggatctac tgcacgagct tcacgaacgg
     5341 ccaagtgaac atcgggcccg gcgacttcac gttctacgtg aagaacggct tcctcatcga
     5401 ggacgggaag ctcacgcggc ccatcaagga cgtcaacatc atcgggaacg gcccgaaggt
     5461 cctcgagaag gtggacatgg tctccgacga cctcaagatc g
//
LOCUS       DAOMFY010000267         5457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5183_length_5457_cov_7.785635, whole genome shotgun sequence.
ACCESSION   DAOMFY010000267 DAOMFY010000000
VERSION     DAOMFY010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5183_length_5457_cov_7.785635"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1717)
                     /locus_tag="PLB01_16380"
     CDS             complement(41..1717)
                     /locus_tag="PLB01_16380"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HQQ78931.1"
                     /translation="MNKPPAARVFVLLALAALAVPALAPAPALASSHNEAPFTSKNPA
                     LDDTDFYMFRDPNDPSMVNLIACRYGLIEPQGGPNYAGFQDGARYDIKIDNNGDGVED
                     VTFRFTFKTTYAQPNSFLYALPGLTSITDSNLYVKQTYSLDRIAGPAANPSAGQITNI
                     LTDQPVFPPNIGPKTFPNYVSLPNGIQKSADGKISVFVGPRQDPFFVDLGMIFDAVNL
                     EVGAGNGRPGVGVGNTGGGRDTLYGYTVLATALQVPIAMLTSAGIQVNDPNNPVSIVG
                     GWASVSIPSTRTLNADGTVTSGTTYQQVSRLGNPLVNEVLVGVGRKDSWNASTPAQES
                     AFKGDATTPGELYPILATYMNVLFGVPVPPGPRGDLELALFRGLPGSVASLFGVSPTQ
                     RPGEVYSDQLRLNTAVAPTPFASASRLGYLGGDPAGFPNGRRMCDDVVDIELRAVAGI
                     IVGLLPPASGGNPIYAASPNKDLGDGVDFPQRGCRYFFPYMWSPTSGFSALHAGAPAP
                     IKSVGSVRAIKEPSVKGFPEGISDEEFAERVRIARDRDDLARIPTTKDAR"
     gene            1891..1989
                     /locus_tag="PLB01_16385"
     tRNA            1891..1989
                     /locus_tag="PLB01_16385"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1925..1927,aa:Sec,seq:tca)
     gene            2093..2623
                     /locus_tag="PLB01_16390"
     CDS             2093..2623
                     /locus_tag="PLB01_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78932.1"
                     /translation="MKRSILTLALAGFVGFGLTAVAEDAKKPAPKTITETTTAKVTVL
                     DINKAKKEVTVKDEAGDVSVVEVPEHFTLEKVKVGDVVTLAYAETLSVGLAKPGEMDS
                     VAAKKTTSGGAAYVTKVVTIAALDMAVPSVTFKTADGKTQTVKIKNAKNAEGYKVGDK
                     VALTYTGALTISIDKK"
     gene            complement(2681..4636)
                     /gene="acs"
                     /locus_tag="PLB01_16395"
     CDS             complement(2681..4636)
                     /gene="acs"
                     /locus_tag="PLB01_16395"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQQ78933.1"
                     /translation="MTAESTAKPGLDLSHSQQVAIEKLAKDENVIHPPIWMARDAVIR
                     NDVVEREHALLSPDDFWAEKATAIEWMEPWKEVCRFQPPNHQWFVGGKLNATVSCIDR
                     HVYGEKRNKAALLWVGEDGEEHAYTYSRLYREVNRFANALKRLGVKKGDRVIIYMPLT
                     PEGVVTMLSCARIGAIHSVVFAGMGTQALKSRIEDCRAKVVVCSDFTFRRGKKIPLKP
                     TIDEAVRDLPFVEKVVVHRRGSRPGDEPVSFESERECDFYDIQHAREIHCPPEPMDAE
                     DPLFILYTSGTTGTPKGVVHTVGGYMVGVTYLARAFYQIGDRDIYWSTSDIGWIVGHS
                     FIVYGPLSVGATIFTREGVPDYPSADVTWELCERVGVNILFTAPTAMRMFMSHGAEAP
                     ARYDLSRLRLIACAGEPLNPEAHLWAQKHLVGQSGGMVVDNFWQTEIAAPVLGTLPTF
                     EARPGKVGKPLPGVAADIVDKDGKSLPDGHGGLLVLRRPVPYMLRTVWGDPARYEKYW
                     ETIPGCYTCGDIAVRDHDGYFAVLGRSDDVMNVAGHRIGTADVEGSLLRHPAVAESAV
                     VGLPDALKGERIKAFVVLKATAQAGAGLIGSLKDHVRQDLGPIAVPQDIDLVPSLPKT
                     RSGKIVRRYLKAKEMGEDPGDLSTLAD"
     gene            4771..>5457
                     /locus_tag="PLB01_16400"
     CDS             4771..>5457
                     /locus_tag="PLB01_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQQ78934.1"
                     /translation="MRALRLHRPAPVAARPLVLEEAPDPVPGPGEVLVSVAACGVCRT
                     DLHVVEGDLPVRRSPVVPGHQAVGTVVRAGGGADALLGRRVGVAWLHAACGACRFCRS
                     GRENLCLEPEFTGWTVDGGFADLLVCRAEFVYELPDGFSDLAAAPLLCAGIIGFRALR
                     LTGLGARGGFGGARLGIYGFGAAGHVAIQFARARGAEVYVMTRDRERHQALALELGAA
                     WVGGAADRPPV"
BASE COUNT          813 a         1786 c         1986 g          872 t
ORIGIN      
        1 cgcgagaagc gggagcggcc ggaaccggaa cggggaacgg ctagcgcgca tccttggtcg
       61 tcgggatgcg ggcgaggtcg tctcggtcgc gggcgatccg aacgcgctcg gcgaactcct
      121 cgtcggagat gccttcgggg aagcccttga cggacggctc cttgatggcg cggacggacc
      181 cgaccgactt gatcggagcc ggggcgcccg cgtggagcgc cgagaaaccg ctcgtcggcg
      241 accacatgta ggggaagaag tagcggcagc cgcgctgcgg gaagtccacg ccgtcgccga
      301 ggtccttgtt gggcgaggcg gcgtagatcg ggtttccgcc ggaggccgga ggaagcagtc
      361 ccacgatgat gccggcgacg gcgcggagct cgatgtccac gacgtcgtcg cacatgcggc
      421 gcccgttcgg gaaccccgcc gggtcgccgc cgaggtagcc gagccggctc gcggacgcga
      481 acggcgtcgg cgcgacggcc gtgttcagcc ggagctggtc ggagtagacc tcgcccgggc
      541 gctgcgtcgg cgagacgccg aagagcgacg cgacggagcc cgggaggccg cggaacagcg
      601 cgagctcgag gtcgccgcgc gggccggggg gcaccgggac gccgaagagg acgttcatgt
      661 acgtggcgag gatcggatag agctcgccgg gcgtcgtcgc gtcccccttg aatgcggatt
      721 cctgcgccgg cgtcgaggcg ttccaggaat ccttgcggcc cacgccgacg aggacttcgt
      781 tcacgagcgg gttgccgagc cgcgagacct gctggtaggt cgtgccgctc gtcacggtcc
      841 cgtccgcgtt gagggtgcgc gtggacggaa tgctgacgga ggcccagccc cccacgatcg
      901 agaccgggtt gttggggtcg tttacctgga tccccgccga ggtgagcatc gcaatcggga
      961 cctggagggc cgtggcgagg acggtgtagc cgtagagcgt gtctctaccg ccgcccgtgt
     1021 tcccgacgcc cacgcccgga cggccgttgc ccgctccgac ctcgaggttc acggcgtcga
     1081 agatcatgcc gaggtcgacg aagaacgggt cctgccgcgg cccgacgaag accgagatct
     1141 tcccgtcggc gctcttctgg atgccgttgg ggaggctcac gtagttcggg aacgtcttgg
     1201 ggccgatgtt cggcggaaag accggctggt cggtgaggat gttcgtgatc tggccggcgg
     1261 acggattcgc cgcggggccc gcgatccggt cgaggctgta ggtctgcttc acgtagaggt
     1321 tcgaatccgt gatcgacgtc aggcccggca gcgcatagag gaacgagttc ggctgcgcgt
     1381 acgtggtctt gaacgtgaag cggaaggtca cgtcctcgac gccgtcgccg ttgttgtcga
     1441 tcttgatgtc gtaccgggcg ccgtcctgga agcccgcgta gttcgggccg ccctggggct
     1501 cgatgaggcc gtagcggcag gcgatgaggt tgaccatcga cgggtcgttc ggatcccgga
     1561 acatgtagaa gtccgtgtcg tcgagggccg ggttcttgga cgtgaacggc gcctcgttgt
     1621 ggctcgacgc gagggcgggc gccggcgcga gggccggaac cgcgagggcg gcgagcgcga
     1681 gcaggacgaa cacacgggca gcgggcggtt tgttcatcgg tctccctttc gttcggatcg
     1741 agatatttcg ttgaaagtgt cgcgcgtcgc gaacaggacc ggtctcgtcg tttcctgaca
     1801 ggggatacgc gggccggggc tccgccggat cagcggaggg atcggagcgc gaccggagcg
     1861 tgacgccgcg caccgtctag aatccgcccc ggaagcgcat ggggcccggc ggcctccctg
     1921 gtcttcaaaa ccagcgtgtc cgctccggaa ggggcggcgg gtgggttcga ctcccacacg
     1981 cttccgccaa tccacctgat gcaggcggac cggatcaggc gcggacgggg tgtcgtatgc
     2041 tcccggaccg cctgctcggg catcgcccga tcaataacag aaggagttcc tcatgaagcg
     2101 ttcgatcctc acgctcgccc tcgccggttt cgtcggattc ggcctcacgg ccgtcgccga
     2161 ggacgcgaag aagcccgccc cgaagaccat cacggagacg acgaccgcca aggtcacggt
     2221 cctcgacatc aacaaggcca agaaggaagt cacggtcaag gacgaggccg gcgacgtgtc
     2281 cgtcgtcgaa gtccccgagc acttcaccct cgagaaggtc aaggtcggcg acgtcgtgac
     2341 gctcgcgtac gccgagacgc tctccgtcgg cctcgccaag ccgggcgaaa tggattccgt
     2401 cgcggccaag aagacgacga gcggcggagc ggcctacgtg acgaaggtcg tcacgatcgc
     2461 ggcgctcgac atggcggtcc cgtccgtcac gttcaagacg gccgacggca agacccagac
     2521 cgtcaagatc aagaacgcca agaacgccga gggctacaag gtcggcgaca aggtcgccct
     2581 gacctacacc ggcgcgctca cgatctcgat cgacaagaag taaggcgctt cgccggatcg
     2641 gacgcccccg gcggcccgtg ccgccggggg cgtttccgtt tcagtcggcc agcgtcgaga
     2701 ggtcgcccgg gtcctctccc atctccttgg ccttgaggta acgcctcacg atcttccccg
     2761 accgcgtctt cggaagggac gggacgaggt cgatgtcctg gggaaccgcg atgggtccga
     2821 ggtcctgccg cacgtggtcc ttgaggctgc cgatgaggcc cgcgcccgcc tgggcggtgg
     2881 ccttcaggac gacgaacgcc ttgatcctct cgcccttgag ggcatccggc aggccgacga
     2941 ccgcgctctc ggcgacggcc ggatgacgca ggagggagcc ctcgacatcg gccgtgccga
     3001 tccggtggcc cgcgacgttc atgacgtcgt ccgagcggcc gagaacggcg aagtagccgt
     3061 cgtggtcgcg cacggcgatg tcgccgcacg tgtagcagcc cgggatcgtc tcccagtact
     3121 tctcgtagcg cgccggatcg ccccacacgg tgcggagcat gtacgggacg ggtcgcctga
     3181 ggacgagaag gccgccgtgc ccgtcgggca gcgacttgcc gtccttgtcc acgatgtccg
     3241 ccgcgacgcc cggaagcggt ttcccgacct tgccgggccg cgcctcgaac gtcgggagcg
     3301 ttccgaggac gggggccgcg atctccgtct gccagaagtt gtcgacgacc atgccgcccg
     3361 actgcccgac gaggtgcttc tgcgcccaga gatgggcctc ggggttgagg ggttcgcccg
     3421 cgcacgcgat gaggcggagg cgcgagaggt cgtagcgcgc gggcgcctcg gccccgtggc
     3481 tcatgaacat gcgcatcgcc gtcggcgcgg tgaagaggat gttcacgccc acgcgctcgc
     3541 agagttccca ggtgacgtcg gccgacgggt agtccggaac gccctcgcgc gtgaagatcg
     3601 tggcgccgac ggacagcggt ccgtagacga tgaacgagtg gccgacgatc cagccgatgt
     3661 cggaggtgga ccagtagatg tctcggtcgc cgatctggta gaaggcccgc gcgagatacg
     3721 tgacgccgac catgtagccg cccaccgtgt ggacgacgcc cttgggcgtt cccgtcgtcc
     3781 cggacgtgta caggatgaag agcgggtcct cggcgtccat cggctcgggc gggcagtgaa
     3841 tctcgcgcgc gtgctggatg tcgtagaagt cgcactcgcg ctcgctctcg aaactaacgg
     3901 gttcgtcgcc cggcctcgag ccgcggcggt ggacgaccac cttctcgacg aaggggaggt
     3961 cgcggacggc ctcgtcgatc gtcggcttga gcggaatctt cttgccgcgc cggaacgtga
     4021 agtccgagca gacgacgacc ttggccctgc agtcctcgat gcgggacttg agcgcctgcg
     4081 ttcccattcc ggcgaagacc acggaatgga tcgcgccgat gcgcgcgcac gagagcatcg
     4141 tgacgacgcc ctccggcgtg agaggcatgt agatgatgac gcggtcgccc ttctttacgc
     4201 cgaggcgctt cagggcgttc gcgaaccggt tcacctcacg gtagagccgg ctgtacgtgt
     4261 acgcgtgctc ctcgccgtcc tcgccgaccc acaggagcgc cgccttgttg cgcttttcgc
     4321 cgtagacgtg ccggtcgatg cacgagacgg tggcgttgag cttgccgccg acgaaccact
     4381 ggtggttcgg cggctggaag cggcagacct ccttccaggg ctccatccac tcgatggccg
     4441 tggccttctc ggcccagaag tcgtccggcg agagaagcgc gtgctcgcgc tcgacgacgt
     4501 cgttgcggat gacggcgtcg cgcgccatcc agatgggggg atggatgacg ttctcgtcct
     4561 tcgcgagctt ctcgatcgcc acctgctggg aatgcgagag gtcgaggccg ggtttcgccg
     4621 tcgattccgc cgtcatggga cgctccttgt ccggtggatt tgcgccgatc ttatgtcgtg
     4681 tcagtcgaga ccgagtcgcg cctgcgttca atgaagcagc cgcatcattc cgggctggcg
     4741 ggagaggacg caggggccta ggttcgtgat gtgcgcgcgc tccgtctgca ccgtcccgcg
     4801 cccgtcgccg cgaggccgct cgtcctcgag gaggcgcccg atcccgtccc gggcccgggc
     4861 gaggtcctcg tctccgtcgc ggcgtgcggc gtctgccgga ccgatctcca cgtcgtcgag
     4921 ggcgacctgc ccgtccgtcg ttctccggtc gttcccgggc accaggccgt cgggacggtc
     4981 gtgcgcgccg ggggcggcgc ggacgcgctc ctcgggcgcc gggtcggcgt cgcgtggctc
     5041 cacgcggcct gcggcgcgtg ccgcttctgc cgctcaggcc gcgagaacct ctgtctcgag
     5101 ccggaattca cgggctggac ggtggacggc gggttcgcgg acctcctcgt ctgccgggcg
     5161 gagttcgtct acgagctgcc ggacgggttc tccgacctcg cggccgcgcc cctcctctgc
     5221 gccgggatca tcgggttccg ggcgctgcgc ctgacggggc tcggcgcgcg cggaggattc
     5281 ggcggcgcgc ggctcgggat ctacggcttc ggggccgccg gccacgtcgc gatccagttc
     5341 gcgcgcgcgc gtggagccga ggtctacgtc atgacgcggg accgcgagcg gcaccaggcg
     5401 ctcgccctcg agctcggcgc cgcgtgggtc gggggcgcgg ccgaccggcc gcccgtc
//
LOCUS       DAOMFY010000268         5446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5196_length_5446_cov_9.550918, whole genome shotgun sequence.
ACCESSION   DAOMFY010000268 DAOMFY010000000
VERSION     DAOMFY010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5196_length_5446_cov_9.550918"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLB01_16405"
     CDS             <1..577
                     /locus_tag="PLB01_16405"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HQQ78935.1"
                     /translation="ALAGWSALAAAAPGVTGRLEFEAVVRGRARGEAERVVEAGLRGE
                     AFRLWKAGGYGGDRSERAAWVVQAAAGVAWLDWPWDRRYLASRWLGPAPVGAMAIVHT
                     HPAVVDPRPSDTDRATANRLGVPVYTVSRRGIWKAEPGGAVTRVGDETWWSGCETGEA
                     CREGAPAGVVRASQEFPAPVETAARPLRITE"
     gene            577..1356
                     /locus_tag="PLB01_16410"
     CDS             577..1356
                     /locus_tag="PLB01_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQQ78936.1"
                     /translation="MRPNVRTLIVDDEPLARERLRTLLADEPGVEVVGECGDGCQAVV
                     AIEELKPDLVFLDVQVPNLDGFRILESVGADRIPAIVFVTAYDHYALRAFDVRAVDYI
                     LKPFGRERLQKALEHARSQITREKSTDISGQLVALLEEIRPQKSYLKRIMIKSGGRLY
                     FLRTADVDWVEAAGNYVRLHVGAESHLLRETMNGLETKLDPERFIRIHRSTMVNIERI
                     KELQPWFHGDYVVILRDDRQLTMSATYREKLDELRGRALPA"
     gene            1528..4548
                     /locus_tag="PLB01_16415"
     CDS             1528..4548
                     /locus_tag="PLB01_16415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ78937.1"
                     /translation="MRHRCRVVLFLFLAAFAAGSALAQTTGDLDGTVSDQNGGPLPGA
                     SVELRSPSLQGARTVTTDAAGRYRFPALAPGVYAVTAKLAGFSSAERTGLKVSLGATT
                     TASLQLAVSVKESLVVTAEAPAIDTTRTTIGTNATLDTMQRLPIGRNFVSIANTVSGT
                     GTDVTGNVTVYGATGLENAYIIDGVNTTGVKTGTQAKSLNNEFVQEVEVKTGGYEAEY
                     GRVLGGTINVVTKSGGNEFHGDAFGYYDGSSLASSDKRSADRAAANQGQYYTPKRVDF
                     GADLGGYFVKDRVWFFGAFDRVTQDQDYTRTLAQIRTGAGGTVLTSDTDTNRNNLYSG
                     KFTFRLGESHTIAASVFGDPGTFHGRYDLTQIPNMIGDSGAFVVDRNVGGTDGSLKYD
                     GILGTSFLLQGQIGYHTEKRDDSSPVAGSPYREVQQAGFSTEALPGSGPILLLNEKYQ
                     RYVYKLAGSVFAGSHEIKAGIDWEHLNSDFTESYGGTDRIRTRLAADGSLRNYQHRYF
                     AQTPLSGANCVGKIDPTGPAAFPNCTGYTIAPTVDNNPITDNAALFVQDSFKPLKNLT
                     INAGLRYEQQSLKDYTGTTLVKVTDEWAPRLGLVWDFMNNGRSKLYANAGRFYEVIPQ
                     DIQTRALGNEYIIITRNSSSTPNPVDLFLGGPVVQGGELTQNGLKGMYQDEVILGFEY
                     EVAKNWAIGVKGIYRALGRVVEDRCDLAINPDLAGYFNPASPATCALINPGQGDSLGA
                     IKDPSDPRCYPNGSTDAAGNLVASAPCDSTEARRYFRGLEVTASHRFSNNFYLLASYL
                     YSQLEGNYSGNLSQTREGGQQDPNINADFDYPGLLVNAYGRLRNDRAHQVKVSGYYAF
                     PFGLTVGANANFATGRPYSIRGCAADVTACGAGYNQEGYLVPRGSAGDLPSTFEADLH
                     LEYGFRIGGLTVTPLLDVFNLINRQGVTSREELFNNTSGLAGNDPRSGIGQPGCTAQN
                     ASLSNAACASNPTYGKDINWQSPRVVRVGARLSF"
     gene            complement(4604..>5446)
                     /locus_tag="PLB01_16420"
     CDS             complement(4604..>5446)
                     /locus_tag="PLB01_16420"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HQQ78938.1"
                     /translation="ELPARAAGAPAAAPAQQPAGQVRQPRGLASDAKGRLYVADFGNV
                     RVQAFKPDLSPLFSVGSLGSGPAEFRDPCGVAVGANGLIYVADTWNGRIQVLDEKGAF
                     QREFSADFFGPRGVAVDAKGTVFVADTGNSRIVRFDGEGRKEAAWGREPGPGKLAEPQ
                     GLALGRDGLLYVADNGNGRVAVFTRDGAFVRAWDVPGWRRQVMSEPYLAVDASGRVWV
                     SVPLEGEVRGYAPDGRLLATARGKDQPEGRRFEHPSGVALLPDGRLAVADFEGRLVVI
                     SLPK"
BASE COUNT          889 a         1979 c         1842 g          736 t
ORIGIN      
        1 cgcgctcgcc ggatggagcg cgctcgcggc agcggcgccc ggcgtcaccg gccggcttga
       61 gttcgaggcc gtggtgcgcg gccgcgcgcg cggggaagcc gaacgcgtcg tcgaagccgg
      121 gcttcgcgga gaggcgttcc gcctctggaa ggcgggaggg tacggcgggg accgctccga
      181 gcgcgccgcg tgggtcgtgc aggccgcagc gggcgtggcg tggctggact ggccctggga
      241 ccgcaggtac ctcgcgtcgc gctggctcgg tccggcgccc gtcggggcga tggccatcgt
      301 ccacacgcat cccgcggtcg tcgacccgcg gccgtcggac acggaccgcg ccacggcgaa
      361 ccgactcgga gtccccgtct acacggtgtc ccggagaggg atctggaagg ccgagcccgg
      421 cggagccgtg acgcgcgtcg gggacgaaac ctggtggtcg gggtgcgaga ccggggaagc
      481 ctgccgtgaa ggggcgccgg ccggggtcgt acgggcttcc caagaatttc ccgcgcccgt
      541 ggaaaccgcg gcgcgcccgc tgcgtatcac ggagtgatgc gcccgaacgt ccgcacgctc
      601 atcgtcgacg acgagcctct cgcccgcgag cgcctgcgca cgctcctcgc ggacgagccg
      661 ggcgtcgagg tcgtcgggga atgcggcgac ggctgtcagg ccgtcgtcgc catcgaggag
      721 ctcaagccgg acctcgtctt cctcgacgtc caggtcccga acctcgacgg cttccgcatc
      781 ctcgagagcg tcggcgcgga ccgcatcccc gccatcgtct tcgtgaccgc ctacgaccat
      841 tacgcgctgc gggccttcga cgtgcgcgcc gtcgactaca tcctcaagcc gttcggccgc
      901 gagcgactcc agaaggcgct cgagcacgcg cgctcccaga tcacgcgcga gaagtcgacg
      961 gacatctccg ggcagctcgt cgccctcctc gaggagatcc gcccccagaa gagctacctc
     1021 aagcggatca tgatcaagtc cggcgggcgc ctgtacttcc tgcggacagc cgacgtggac
     1081 tgggtcgagg cggcgggcaa ctacgtgcgc ctccacgtcg gcgccgagtc ccacctcctg
     1141 cgcgagacga tgaacggcct cgagaccaag ctggacccgg agcgcttcat ccggatccac
     1201 cgctccacga tggtgaacat cgagcggatc aaggagctcc agccctggtt ccacggcgac
     1261 tacgtcgtga tcctccgcga cgaccgccag ctcacgatga gcgcgacgta ccgcgagaag
     1321 ctcgacgagc tccgcggccg cgcgctgccc gcctgaggcg cgccgcgccc gcaccaaagt
     1381 cttgaatgag gggggcggcg gtcccgcgcc cgggaaatcc cgcccccgcg tccctcccgc
     1441 ccgtcccaaa ccagatgtga ggggacgagc ccggacgcac tctgcccggc aggaatcggc
     1501 ccttcgccga tccgcgggag gagttttatg cgtcatcgat gtcgcgtcgt gttgttcctt
     1561 ttcctcgcgg cgttcgccgc aggctccgcg cttgcgcaga ccacgggcga tctggacggg
     1621 accgtctccg accagaacgg agggcctctg ccgggcgcga gcgtcgagct gaggtcgccg
     1681 agcctgcagg gagcccggac cgtcacgacg gacgccgccg gacggtaccg gttcccggcg
     1741 ctcgcccccg gtgtttacgc ggtgacggcg aagctcgcgg gcttctcgtc ggccgagcgc
     1801 acgggcctga aggtctcgct cggcgcgacg acgacggcct cgctgcagct cgcggtgtcc
     1861 gtgaaggaga gcctcgtcgt cacggccgag gcgcccgcga tcgacacgac gcggacgacg
     1921 atcgggacga acgcgacgct cgacacgatg cagcgcctcc cgatcgggcg gaacttcgtc
     1981 tcgatcgcga acaccgtctc cgggacgggc acggacgtga cgggcaacgt cacggtctac
     2041 ggggcgaccg ggctcgagaa cgcgtacatc atcgacggcg tcaacacgac gggcgtcaag
     2101 accggcacgc aggcgaagtc cctcaacaac gagttcgtcc aggaggtcga ggtcaagacc
     2161 ggcggctacg aggccgagta cggccgcgtc ctcggaggaa cgatcaacgt cgtgaccaag
     2221 tccgggggca acgagttcca cggcgacgcg ttcgggtact acgacggctc gtcgctcgcg
     2281 tcgagcgaca aacgctccgc cgatcgcgcc gcggcgaacc aggggcagta ctacacgccg
     2341 aagcgcgtgg acttcggcgc ggacctcggc ggctacttcg tcaaggaccg cgtctggttc
     2401 ttcggggcgt tcgaccgcgt gacgcaggac caggactaca cgcgcacgct cgcccagatc
     2461 cggaccggtg cgggaggcac ggtcctgacg tccgacacgg acacgaaccg gaacaacctg
     2521 tattccggta agttcacgtt ccgcctcggc gagtcgcaca cgatcgccgc gtccgtgttc
     2581 ggggatccgg gcacgttcca cggccggtac gacctgacgc agatcccgaa catgatcggc
     2641 gacagcgggg cgttcgtcgt ggaccgcaac gtgggcggca cggacgggtc gctgaagtac
     2701 gacggcatcc tcggcacgtc cttcctcctg cagggccaga tcggctatca cacggagaag
     2761 cgcgacgact cgagccccgt cgcggggagc ccgtaccgcg aggtccagca ggccggcttc
     2821 tcgacggagg cgctgccggg cagcgggccg atcctcctcc tgaacgagaa gtaccagcgc
     2881 tacgtctaca agctcgcggg ctccgtcttt gccggcagcc acgagatcaa ggccgggatc
     2941 gactgggagc acctgaactc agacttcacg gagtcctacg gaggcacgga ccgcatccgc
     3001 acgcgcctcg cggccgacgg ctcgctccgc aactaccagc accgctactt cgcgcagacg
     3061 ccgctctccg gcgcgaactg cgtcggcaag atcgacccga cggggccggc cgcgttcccg
     3121 aactgcacgg gctacacgat cgccccgacg gtcgacaaca acccgatcac ggacaacgcg
     3181 gccctcttcg tccaggactc gttcaagccc ctgaagaacc tcacgatcaa cgccggcctc
     3241 cgatacgagc agcagtccct caaggactac acgggaacga cgctcgtcaa ggtcacggac
     3301 gagtgggccc cgcgcctcgg cctcgtgtgg gacttcatga acaacggccg atccaagctc
     3361 tacgcgaacg cggggcgctt ctacgaggtc atccctcagg acatccagac gcgcgcgctc
     3421 ggcaacgagt acatcatcat cacccgcaac tcgagctcga cgccgaatcc cgtcgacctg
     3481 ttcctcggcg gcccggtcgt gcagggcggc gagctgacgc agaacggcct caagggcatg
     3541 taccaggacg aggtcatcct cggcttcgag tacgaggtcg cgaagaactg ggcgatcggc
     3601 gtcaagggca tctaccgggc gctcgggcgc gtcgtcgagg accgttgcga cctcgcgatc
     3661 aacccggacc tcgcgggcta tttcaacccg gcgagcccgg cgacgtgcgc gctcatcaac
     3721 ccgggccagg gcgactccct cggcgcgatc aaggacccga gcgacccgcg ctgctacccg
     3781 aacggctcga ccgacgcggc gggcaacctc gtcgcgagcg ccccgtgcga ctcgacggag
     3841 gcccgccgct acttccgcgg tctcgaggtc acggcgtccc atcgtttctc gaacaacttc
     3901 taccttctcg cgagctacct ctactcacag ctggaaggca actactccgg aaatctctcg
     3961 cagacgcgcg agggcggcca gcaggacccg aacatcaacg ccgacttcga ctaccccggc
     4021 cttctcgtga acgcctacgg acggctccgg aacgaccgcg cacaccaggt gaaggtctcc
     4081 ggctactacg cgttcccgtt cggcctcacg gtcggcgcga acgcgaactt cgcgacgggg
     4141 cggccctact cgatccgcgg ctgcgccgcg gacgtcacgg cgtgcggcgc cggctacaac
     4201 caggagggct acctcgtgcc gcgcggctcg gcgggagacc tcccgtccac gttcgaggcg
     4261 gacctccacc tcgaatacgg cttccgcatc ggagggctca ccgtcacgcc gctcctggac
     4321 gtcttcaacc tcatcaaccg ccagggcgtg acgagccgcg aggagctctt caacaacacg
     4381 agcggcctcg ccgggaacga cccgcggagc ggcatcgggc agccgggctg cacggcgcag
     4441 aacgcgagcc tttcgaacgc ggcatgcgcc tcgaacccga cgtacggcaa ggacatcaac
     4501 tggcagtcgc cgagagtcgt gcgcgtgggc gcgcgcctct cgttctgaag cgggcccacg
     4561 acctcctgaa ccacggggcc ggccgggggc cggccccgct ttttcacttc ggcagcgaga
     4621 tcacgacgag gcggccctcg aagtccgcga cggcgagccg cccgtccggg agcagcgcga
     4681 ctccggaggg gtgctcgaag cgccggccct cgggctggtc cttcccgcgt gcggtcgcga
     4741 ggagccttcc gtccggcgca tacccccgga cctctccttc gagcggcacc gagacccaga
     4801 cgcggcccga cgcatcgacg gcgaggtacg gctcgctcat gacctgccgg cgccagccgg
     4861 gcacgtccca cgcgcggacg aaggcgccgt cgcgcgtgaa gacggccacg cgcccgttgc
     4921 cgttgtcggc gacgtagagg aggccgtccc tgccgagcgc gaggccctgc ggctcggcga
     4981 gcttgcccgg gcccggctcc cggccccacg cggcttcctt gcgcccctcg ccgtcgaacc
     5041 gcacgatgcg gctgttcccc gtgtcggcca cgaagaccgt gcccttcgcg tccaccgcga
     5101 cgccgcgcgg cccgaagaag tccgcggaga actcgcgctg gaacgcgcct ttttcgtcga
     5161 gcacctggat gcggccgttc cacgtgtccg cgacgtagat caggccgttc gcgccgacgg
     5221 cgacgccgca cgggtcccgg aactcggcgg ggccggagcc gagcgacccg acggagaaga
     5281 ggggcgacag gtcgggcttg aacgcctgca cgcgcacgtt gccgaagtcg gcgacgtaga
     5341 ggcgcccctt cgcatccgac gcgaggccgc gcggctggcg gacctgcccg gcaggctgct
     5401 gcgcgggagc ggcggccgga gcgcccgccg cacgcgccgg cagttc
//
LOCUS       DAOMFY010000269         5438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5212_length_5438_cov_7.208805, whole genome shotgun sequence.
ACCESSION   DAOMFY010000269 DAOMFY010000000
VERSION     DAOMFY010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5212_length_5438_cov_7.208805"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLB01_16425"
     CDS             <1..237
                     /locus_tag="PLB01_16425"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HQQ78939.1"
                     /translation="RPLILFLRKSSLLSYSRAHPKALVLSLGSSPEEMAKNLFAALRT
                     RRKGTELLVLAVPRRGVGRAVMDRLTRAATRIVP"
     gene            complement(234..1793)
                     /locus_tag="PLB01_16430"
     CDS             complement(234..1793)
                     /locus_tag="PLB01_16430"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HQQ78940.1"
                     /translation="MSKETNTMNELDKRVAELAEKYRPIAAKILKDVIRIPADYVDRP
                     KDAGGDPSCGLSNHEKPRLDVLKKAILEIGAVRRPEDVGFDGYGNLVWTVEDPSDGIP
                     AKDKRVVYFDGHTDTVRPLRSAWREKTGGIDAFEGLFDAAAVRRDFLEAELGHLPPSS
                     EWEHLVFGRGGADQLSGVVTQVVATKILLELAPLGALKGAIVRSYATVAEEDNDGGGP
                     QYLVKKVLPGAGPELVPDAVILTEGTGDSAKGALGIYRGQRGRMQIEVSVTGKSCHGS
                     MPWEGKNPLEWAGPILAEAAKRYDARDGFLDHPFLGHGTRTASWARLDTPSDCAVPER
                     FVFRFDRRLTIGETPEKAVADVEGLTAVAAARAAGLTVTVTVPTYDEPTWTGFVLHNP
                     QVYMGWLTPEEHPAVQAAVAAYRGVVSPHVPEGGVRGTLRKEPRVDRWIFSTDGVGFP
                     VPKDDTSIAVPERKRWVVSGAVKHPAMLGIGPGIEQNTHKIGECVDLREVVHATAMLA
                     RFPSAFVAASG"
     gene            complement(1790..2917)
                     /locus_tag="PLB01_16435"
     CDS             complement(1790..2917)
                     /locus_tag="PLB01_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HQQ78941.1"
                     /translation="MTKTATIDPAAAERLRGRSLLLTDDASAEELSALLAVAERFAAL
                     DRAGKKAALLPHELAYAMFFDNSTRTKSAWAGASARLGMHPVIVDGSSTQVSHGETAA
                     ETGAMLGMNAHALGVRHDLILGEGNAFMRDVKKGIDDYLAATGDPRSVPVVNLQCDID
                     HPTQTLADLCWLREKFPEGLAGKRIAVSWAYSPSYAKPLSVPQGLIMLMTRFGAHVTL
                     AHPEGYRLMDPCLASAAAHAATSGGSFRTTSSMDEAFEGADVVYPKSWGPHDLMLQRV
                     DANRARDTKRMGEIEKEALARNAGHKDWICDERRMKATKGGDALYMHCLPADIGAEVS
                     PGVMAKHVVNVAREANWKVYVVMALLAAAKVPDLARRLEAL"
     gene            complement(2914..4905)
                     /locus_tag="PLB01_16440"
     CDS             complement(2914..4905)
                     /locus_tag="PLB01_16440"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ78942.1"
                     /translation="MLRPLPLSVLVRRAAEELPKGAVYDLPVRHVYRGVPGLDLSARF
                     HGSRAATPLGPAAGPQTQLAQNIALSYLAGGRILELKTVQVNDHLVIPRPCIDMRTVG
                     FNVEWSQELLVEDSLREYAKARLLVAILGELCGIPENERDVIFDVSVGYDLEGIRSPK
                     MSAFFDGIRDAGVILDEERAVLRRELPGSLRRFADVPCPARLSDSVTLSTFHGCPAGE
                     IDAIGRHLLAERGLHTIVKLNPTLLGYEEVEEILHGRLGYRHVALRRSAFEKDLHWEG
                     ALAIARGLSAEAARLGLGFGFKVTNTLVVANAGDFMPGDEAYLSGEPLHVLALAVGLR
                     LREELGAELPLSFSAGVDAANVAGAVACGFVPVTSCTDLLRPGGYKRLHRQAEALAGA
                     MRDAGAATRDEFIRKRAGVEGGGALEASLVNHRRAAAASLGDAHYTAARTGTPPRKIG
                     SHLHLFDCINCDKCVPVCPNDANFTYEAPPRKVAYRDLVVEGGRLVPAGEKTLALGGA
                     KSSPHQIANWADACNDCGNCDVFCPEDGGPYIEKPRLFSSLASFLADAPRPGFFLRRE
                     GGATVARGRWGGHEVELLVGTDGTAVFSDGAAELVFAPGGDAPSAARVLRPDVLDGHV
                     VPVGHYHALRALAAGLFAAGAVSWLTATFPDVSSLERST"
     gene            complement(4908..>5438)
                     /locus_tag="PLB01_16445"
     CDS             complement(4908..>5438)
                     /locus_tag="PLB01_16445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ78943.1"
                     /translation="GPKTLLAHGVHLSAAERARAAAAGAWIVHNPRSNMQNAVGYADP
                     KTLGSHVALGTDGMDMDVFAEARAAHLRGREAYGPADGIDAVALLANSQRVADLTLGE
                     RPGDWVVLDYEPPTPLSAGNLAGHVLFGLGTRHVRDVVVNGEVAVRDRVPLRVDAARI
                     RARAREEAARLWGRMA"
BASE COUNT          761 a         1943 c         1938 g          796 t
ORIGIN      
        1 cgtccgctca ttctctttct aagaaaatct tctcttcttt cctactctcg cgcgcatccg
       61 aaggcgctcg tgctgtctct cggttcttcc cccgaggaga tggcgaagaa cctcttcgcc
      121 gcgctgcgca cgcgccgcaa ggggacggag cttctcgtcc tcgccgtccc ccgccgcggc
      181 gtcggccgcg cggtcatgga ccgccttacg cgcgccgcga cgcggatcgt cccctagccg
      241 gacgccgcca cgaacgcgct cgggaagcgc gcgagcattg ccgtcgcgtg gacgacctcc
      301 cgcagatcca cgcactcccc gatcttgtgc gtgttctgct cgatgcccgg gccgatgccg
      361 agcatggccg gatgcttcac ggcgcccgag acgacccagc gcttcctctc tgggaccgcg
      421 atcgacgtgt cgtccttcgg caccgggaac ccgacgccgt ccgtggagaa gatccaccgg
      481 tcgacgcgcg gctccttgcg gagcgtgccg cgcacgccgc cctccggaac gtgcggcgac
      541 acgacgccgc gatacgccgc gacggcggcc tggaccgccg ggtgctcctc gggcgtgagc
      601 cagcccatgt agacctgcgg gttgtggagg acgaagccgg tccacgtcgg ctcgtcgtac
      661 gtcggcaccg tgacggtgac ggtcaacccc gcggcccgcg ccgcggccac ggcggtgagc
      721 ccctccacgt cggcgacggc cttctcgggc gtctcgccga tcgtgagtcg ccggtcgaac
      781 cggaagacga accgctcggg gaccgcgcag tcgctcggcg tgtcgaggcg cgcccacgag
      841 gccgtgcgcg tgccgtggcc gaggaacggg tggtcgagga agccgtcgcg ggcgtcgtag
      901 cgcttcgcgg cctccgcgag gatcggcccg gcccactcca gcgggttctt cccttcccac
      961 ggcatcgagc cgtggcagga cttcccggtg acggacacct cgatctgcat gcggccgcgc
     1021 tggccgcggt agatgccgag ggcgcccttg gcggaatcgc ccgtgccctc cgtgaggatg
     1081 acggcgtccg gaacgagctc cggcccggcg cccggcagga ccttcttcac gaggtactgc
     1141 ggcccgccgc cgtcgttgtc ctcctcggcg acggtggcgt acgaacggac gatcgcgccc
     1201 ttcagcgcgc cgagcggggc gagctcgagc aggatcttcg tcgcgacgac ctgcgtcacg
     1261 acgcccgaga gctggtcggc cccgccgcgc ccgaagacga ggtgttccca ctccgacgag
     1321 ggcggaagat gccccagctc ggcttcgagg aagtcccggc gaacggcggc ggcgtcgaag
     1381 agtccttcga aagcgtcgat cccgcccgtc ttctccctcc aggcggatct caggggacgc
     1441 accgtgtcgg tgtgaccgtc gaagtagacg actctcttgt ccttcgcagg aattccgtcc
     1501 gaggggtctt cgaccgtcca gaccaggttg ccgtagccgt cgaagcccac gtcctcgggc
     1561 cggcggaccg cgccaatctc gaggatcgcc ttcttcaaga cgtcgaggcg gggcttctcg
     1621 tggttcgaga gcccgcagct cgggtcgccg ccggcgtcct tgggacggtc gacatagtcg
     1681 gcgggaatcc ggatcacgtc cttgaggatc ttcgccgcga ttggccggta cttttccgcc
     1741 agctccgcga cccgcttgtc gagctcgttc atcgtgttcg tctccttgct cacagggcct
     1801 ccagccggcg ggccaggtcc gggaccttcg ccgccgcgag gagcgccatc acgacgtaga
     1861 ccttccagtt cgcctcgcgc gcgacgttca cgacgtgctt cgccatgacg ccgggcgaga
     1921 cctccgcccc gatgtccgcg ggcaggcagt gcatgtagag ggcgtcgccg cccttcgtcg
     1981 ccttcatccg ccgctcgtcg cagatccagt ccttgtgccc ggcgttgcgg gcgagcgctt
     2041 ccttctcgat ctcccccatc ctcttcgtgt cgcgcgcgcg gttcgcgtcg acgcgctgga
     2101 gcatgaggtc gtggggaccc cagctcttcg gatagacgac gtcggcgccc tcgaacgcct
     2161 cgtccatgga ggacgtcgtc cggaaggacc cgccggaggt cgcggcgtgc gccgccgcgg
     2221 aagcgaggca cgggtccatg aggcggtagc cctccgggtg cgcgagcgtc acgtgcgcgc
     2281 cgaagcgcgt catgagcatg atgagcccct gcggcaccga gagcggcttc gcgtagctgg
     2341 gcgagtaggc ccacgacacc gcgatgcgct tgccggcgag gccctccggg aacttctcgc
     2401 gcagccagca gagatccgcg agcgtctgcg tcggatggtc gatgtcgcac tgcaggttga
     2461 cgaccggcac gctcctcggg tcgcccgtgg ccgcgagata gtcgtcgatg cccttcttca
     2521 cgtcccgcat gaaggcgttg ccctcgccga ggatcaggtc gtgccgcacc ccgagcgcgt
     2581 gcgcgttcat cccgagcatc gcgcccgtct cggccgcggt ctcgccgtgc gagacctggg
     2641 tggacgagcc gtccacgatg acggggtgca tcccgagccg tgccgaggcg cccgcccagg
     2701 cggacttcgt ccgcgtggag ttgtcgaaga acatcgcgta cgcgagctcg tgcgggagaa
     2761 gcgccgcctt cttgcccgcg cggtccagcg cggcgaagcg ctcggcgacg gcgaggaggg
     2821 ccgagagctc ctccgccgag gcgtcgtccg tcaggaggag gctccggccg cggaggcgct
     2881 ccgcggcggc ggggtcgatg gtggcggtct tcgtcatgtc gatctctcca atgaagaaac
     2941 gtccggaaac gtcgcggtca gccaggaaac ggcgcccgcg gcgaagaggc cggccgcgag
     3001 cgcgcgcagc gcatggtaat ggcccacggg cacgacgtgg ccgtcgagga cgtccgggcg
     3061 gaggacgcgc gccgccgagg gcgcatcgcc accgggagcg aagacgagct cggcggctcc
     3121 gtcggagaag acggccgtgc cgtccgtccc gacgaggagc tccacctcat gcccgcccca
     3181 ccgcccgcgc gccaccgtcg cgccgccctc acgccgcagg aagaaacccg ggcgcggagc
     3241 gtccgcgagg aacgaggcga gggacgagaa gaggcggggc ttctcgatgt acggaccgcc
     3301 gtcctccggg cagaagacgt cgcagtttcc gcagtcgttg cacgcgtcgg cccagttcgc
     3361 gatctggtgc ggcgacgact tcgcgcctcc gagcgccagc gtcttctcac cggccgggac
     3421 gagacggccg ccctcgacga cgaggtcgcg gtacgcgacc ttccgcggcg gcgcttcgta
     3481 cgtgaagttc gcgtcgttcg ggcagacggg aacgcacttg tcgcagttga tgcagtcgaa
     3541 gaggtggagg tgcgacccga tcttccgcgg cggcgtgccc gtccgggcgg ccgtgtagtg
     3601 cgcgtcgccg agggaggcgg ccgccgcgcg gcggtgattc acgagggacg cctcgagggc
     3661 gccgccgccc tcgacccccg cccgcttccg gatgaactcg tcccgcgtcg cggcgcccgc
     3721 gtcgcgcatc gcgcccgcga gggcctcggc ctgccggtgg aggcgcttgt agccgcccgg
     3781 gcgcaggagg tccgtgcagc tcgtgacggg cacgaagccg caggcgacgg cgccggccac
     3841 gttcgccgcg tccaccccgg ccgagaagga caacggaagc tccgcgccca gctcctcgcg
     3901 gagcctcagc cccaccgcga gcgcgaggac gtgcagcggc tcgccggata ggtacgcctc
     3961 gtcgccgggc atgaagtctc ccgcgttggc gacgacgagc gtattcgtca ccttgaaccc
     4021 gaagccgagc ccgaggcgcg cggcttccgc cgagaggccg cgagcgatcg cgagcgcgcc
     4081 ctcccagtgg aggtccttct cgaacgcgga gcggcggagg gccacgtgcc ggtagccgag
     4141 ccgtccgtgg aggatctcct cgacctcctc gtagccgagc agcgtcgggt tgagcttcac
     4201 gatcgtgtgg aggccccgtt cggcgaggag atggcggccg atcgcgtcga tctccccggc
     4261 cggacagccg tggaacgtcg agagcgtgac cgagtccgaa agccgggcgg ggcaggggac
     4321 gtcagcgaag cgccgcaacg atccggggag ctcgcggcgc agcacggcgc gctcctcgtc
     4381 gaggatcacg cccgcgtcgc ggatgccgtc gaagaatgcg gacatcttcg gcgagcgaat
     4441 gccctcgagg tcgtagccga cggagacgtc gaagatcacg tcgcgctcgt tctccgggat
     4501 tccgcacagc tcgccgagga tcgcgacaag gagccgggcc ttcgcgtatt cgcgcagcga
     4561 atcctcgacc aggagctcct gcgaccactc cacgttgaat ccgacggtcc gcatgtcgat
     4621 gcacgggcgc gggatcacga ggtggtcgtt cacctggacg gtcttgagct cgaggatgcg
     4681 cccgcccgcg agatacgaga gcgcgatgtt ctgggcgagc tgcgtctgcg ggccggcggc
     4741 gggccccagg ggcgtcgcgg cgcggctgcc atggaaccgc gcggacaggt cgaggcccgg
     4801 cacgccccgg tagacgtggc ggacgggaag gtcgtagacc gcccccttcg gaagctcctc
     4861 cgcggcgcgg cgcacgagaa cggagagcgg gagaggacgg agcacgctca cgccattcgc
     4921 ccccagagtc gcgccgcttc ctcgcgcgcg cgtgcgcgaa tgcgcgccgc gtcgacgcgc
     4981 agcgggaccc ggtcgcgcac cgcgacctcg ccgttcacga cgacgtcgcg gacgtggcgg
     5041 gtgccgagac cgaacaggac gtgtcccgcg aggttccccg ccgagagcgg ggtcggcggc
     5101 tcgtagtcga gaacgaccca gtcgccgggc ctctccccca gcgtcaggtc ggcgacgcgc
     5161 tggctgttcg cgaggagggc gacggcgtcg atcccgtccg caggcccgta cgcctcccgg
     5221 ccgcgcaggt gcgcggcgcg tgcttccgcg aagacgtcca tgtccatacc gtccgtgccc
     5281 agcgcgacgt gcgagccgag cgtcttcggg tccgcgtacc cgacggcgtt ctgcatgttg
     5341 ctgcgcgggt tgtggacgat ccacgcgccg gccgccgccg cgcgcgcgcg ctcggcggcc
     5401 gacaggtgga cgccgtgcgc gaggagcgtc ttcggccc
//
LOCUS       DAOMFY010000270         5428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5222_length_5428_cov_8.085613, whole genome shotgun sequence.
ACCESSION   DAOMFY010000270 DAOMFY010000000
VERSION     DAOMFY010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5222_length_5428_cov_8.085613"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLB01_16450"
     CDS             complement(<1..369)
                     /locus_tag="PLB01_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78944.1"
                     /translation="MARLLDICTAGPRSDRSPDLVTAEGIAVRVKSASCVTCESRRDL
                     SKIHFTPLPWRAPRRDPACAAPLPHARAYVFALLGHPERTDVNPLDLGEWRFFVPPTA
                     ELEAKMRRQRTLSFSALEALS"
     gene            complement(536..994)
                     /locus_tag="PLB01_16455"
     CDS             complement(536..994)
                     /locus_tag="PLB01_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78945.1"
                     /translation="MPRKIALALLGPALAAVFTAGPALAATEAKPAASNAPQAAPAEG
                     AIKVAKAEGPDGRTVAEVWADRAALKGKPVAVRGKVVKFNAGIMGTNWIHLRDGSGSP
                     AAKDNDLTVTTDAVVKVGDVVTASGAVTVDKDFGAGYAYAVILEGAKITK"
     gene            1136..2179
                     /locus_tag="PLB01_16460"
     CDS             1136..2179
                     /locus_tag="PLB01_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQQ78946.1"
                     /translation="MRTRLASLSLALALLAGGAKASRANPQAADPKAPVARIDGQVIT
                     DGDLTAEVKVSLAAAEARFAEEVWGIKRRALDALVEQRLLDAKAKKEGITADALVARE
                     ITGKVGEPDEAQVKSVYDQTKAAGRQLPPFPEVRAEIAQYLKSQETQQVRQAYVARLK
                     AEAKVQITFPPYLPSRVDVKAEGPSKGSPNAPVTIVEFSDYECSYCAGAEATVKRVLE
                     AYPGKVRLVYRDLPLSIHPNAPKAAEAAHCAGEQQRYWDMHDALFANQQALKVDDLKA
                     HARKLGLDAATFDACLDSGRTAGLVEANRKAGEALWLNGTPTFFVDGRPYVGSRPFET
                     FQEIIDYTLAGKR"
     gene            2286..3080
                     /locus_tag="PLB01_16465"
     CDS             2286..3080
                     /locus_tag="PLB01_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ78947.1"
                     /translation="MSIRAFRARGVFGLFVALTVLAAPRGDAQGVKKRRPLPSEYGRV
                     VMDTASTKAGVPAAVFDHWTHRTRYTCRVCHVDVGFAMQANETRVREDDNRKGFFCGA
                     CHNGKVAFGPKKNGTMDDDPSCVRCHVPAGTTPGLDFAKGTAALPKGRFGNGVDWEAA
                     ESKGLIHPLDALAGVSVVRKPLQIPPDYDIAAKINTLPEIVFSHKKHAVWGGCELCHP
                     DVFTVKRGSTKFTMEEIFKGRYCGACHGTVAFPTTDCQRCHTKPVA"
     gene            3077..3811
                     /locus_tag="PLB01_16470"
     CDS             3077..3811
                     /locus_tag="PLB01_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ78948.1"
                     /translation="MNPAVPALFLLLAGQPWFKLPPHPRPEVFGNVLISRTTAAGPVK
                     PAAFSHWSHRRRHTCRVCHLELEFSFKRNTTPITEEANRQGRFCGGCHDGKTAFAHTE
                     PNCTRCHSGDLAAGAVKFGELRDLQEAPFGNGVDWSAALESGRIAPAASLTKGEGGMS
                     LKSKLSLAAEWAMVPPAIFPHDEHTRWLDCANCHPEPFNIQKKTTKHFSMAKNLAGEF
                     CGACHLTVAFPLDDCKRCHPKMRANS"
     gene            complement(3864..5018)
                     /locus_tag="PLB01_16475"
     CDS             complement(3864..5018)
                     /locus_tag="PLB01_16475"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ78949.1"
                     /translation="MSERLGAPRPGRFHSISTIALGALIAVLAAPGGFAQSMTGIQLH
                     RAPRTPGAGRRALAAPTDGAAYYHNGPTLLCSDCHAAHGSETHGFDGGPVSTTGAPDG
                     DWMPSGAPNGYMLKGTNSTMLCLSCHEGQTFAPDVVDVDVNGLTERAAGYFAPAGVRS
                     HRGHNLTTTSLGKPGDSDLCNRCHFVGTMATAEVQCIDCHNPHGNASYRNLWWASAPG
                     SEPPLLASIKGGTTGLARYEAANVAYPAPSSTDGAWREITNMCIDCHHTFFAPWYTNT
                     ASPYHRHPGTNTESAGYYPINKADANTDPANWVSGTAGFTVARLPFTAVGATSFASAT
                     VVAANNEVFCLTCHKAHGSANPFSLRWDYGTRTATGAAGCQQCHNNVFNE"
     gene            complement(5041..>5428)
                     /locus_tag="PLB01_16480"
     CDS             complement(5041..>5428)
                     /locus_tag="PLB01_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HQQ78950.1"
                     /translation="ETRAEMAARHGQMAAGPAEAADVKVPKAEGANAKTVAEVWASRA
                     ALRGQDVVVKAKVVKVTSGVMGKNWLHVRDGSGSAQKKDHDLTVTTSDVVKVGDVVTI
                     AGAVSVDKDFGAGYAYPVILENAKVK"
BASE COUNT          809 a         1961 c         1842 g          816 t
ORIGIN      
        1 ggacagcgcc tcgagcgcgg agaacgacag cgtccgctgc ctcctcatct tcgcctcgag
       61 ctccgccgtg ggcggaacga agaagcgcca ctcgccgagg tcgagcgggt tcacgtccgt
      121 ccgctccggg tgcccgagga gcgcgaagac gtaggcccgc gcgtgcggga gcggggcggc
      181 gcacgcgggg tcccggcgcg gtgcgcgcca cggcagcggc gtgaagtgga tcttcgagag
      241 gtcgcgccgg ctctcgcacg tcacgcacga cgcggacttc acgcggacgg cgatcccctc
      301 cgcggtcacg aggtcgggcg accggtccga gcgggggccc gcggtgcaga tgtcgagaag
      361 ccgcgccacg atgaactcgg cgagccgggc ccgcgtggaa gtcgcgagca ggtcggcctc
      421 gcaccaccgc cagaagtcga gcagcgtcag gtcgagcctc tcgcccccgg acctgaaggt
      481 ctcgctcccg tcgaggcccc gcggggcggc gagctggttc gcgatcgtcg gccccctact
      541 tcgtgatctt cgcgccttcc aggatgaccg cgtacgcgta tccagcgccg aagtccttgt
      601 ccacggtcac ggcgccgctc gcggtcacga cgtctcccac cttcaccacg gcgtccgtcg
      661 tcacggtgag gtcgttgtct ttcgcggcgg gcgatcccga cccgtcgcgc aggtggatcc
      721 agttcgtgcc catgatcccg gcgttgaact tcacgacctt gccgcgcacg gcgacgggct
      781 tgcccttcag cgcggcgcgg tccgcccaga cctcggccac ggtccggccg tcgggcccct
      841 cagccttcgc gaccttgatc gcgccctcgg ccggcgcggc ctgcggcgcg ttcgacgccg
      901 ccggtttggc ctccgtggcg gcgagcgccg gcccggccgt gaaaacggct gcgagggccg
      961 gtccgaggag agcgagagcg atcttcctgg gcatgggggt tcctttcccg gtgaccgccc
     1021 gggcttcgcc cccggtgccg ttcagcacgg gaatcctagg gatttggcgg cccgtccggc
     1081 atgatcaacc tgaatccctt ccgggaaccc gggccggatg ggaccatccc ccgccgtgag
     1141 aacgagactc gcctcccttt cgctggccct cgccctcctc gcggggggcg cgaaggcgtc
     1201 gcgtgccaac cctcaggccg cggacccgaa agcccccgtc gcgcgcatcg acgggcaggt
     1261 catcacggac ggcgacctca ccgccgaggt caaggtctcg ctcgcggccg cggaagcgcg
     1321 gttcgcggaa gaggtgtggg ggatcaagcg gcgggccctc gacgcgctcg tcgagcagcg
     1381 cctcctcgac gcgaaggcga agaaggaagg gatcacggcg gacgccctcg tggcgcgcga
     1441 gatcacgggc aaggtcggag agcccgacga ggcccaggtg aagtccgtct acgaccagac
     1501 gaaggccgcc ggcaggcagc tgcccccgtt ccccgaggtg agggccgaga tcgcccagta
     1561 cctgaagagc caggagaccc agcaggtccg gcaggcctac gtcgcccgcc tgaaggctga
     1621 ggcgaaggtc cagatcacgt tcccgccgta cctcccgagt cgcgtcgacg tgaaggccga
     1681 aggcccgtcg aaggggagcc cgaacgcgcc cgtcacgatc gtggagtttt ccgactacga
     1741 gtgctcgtac tgcgccggcg ccgaggccac ggtgaagcgc gtcctcgagg cgtacccggg
     1801 caaggtgcgc ctcgtctacc gggacctccc gctctccatc cacccgaacg cgccgaaggc
     1861 cgccgaggcc gcccactgcg ccggcgagca gcagcgctac tgggacatgc acgacgccct
     1921 cttcgcgaac cagcaggcgc tcaaggtcga cgacctcaag gcccacgccc gcaagctcgg
     1981 cctcgacgcc gcgacgttcg acgcgtgcct cgactcgggc cggacggcgg ggctcgtgga
     2041 ggcgaaccgg aaggccggcg aggccctctg gctgaacggc acgcccacgt tcttcgtgga
     2101 cggccgcccc tacgtcggct cgaggccgtt cgagacgttc caggaaatca tcgactacac
     2161 gctcgccggc aagcgctgac cgagcccatc cggctgcccc cgagcagttc gatgcaggtg
     2221 cactacggac ggcggcgccc ttcgaacgga aattaccaag ggccaatccg gcgatgggga
     2281 gtgctatgtc gattcgtgcc tttcgcgctc ggggagtctt cggcctcttc gtcgctctca
     2341 cggtcctcgc cgcgccccgt ggtgacgccc agggcgtgaa gaagcgccgc cccctcccgt
     2401 ccgagtacgg ccgcgtcgtc atggacaccg cctcgacgaa ggccggagtc cccgccgccg
     2461 tcttcgacca ctggacgcac cggacgcgct acacctgccg cgtctgccac gtggacgtcg
     2521 gcttcgcgat gcaggcgaac gagacgcgcg tcagggaaga cgacaaccgc aagggcttct
     2581 tctgcggcgc ctgccacaac ggcaaggtcg ccttcggccc gaagaagaac ggcacgatgg
     2641 acgacgaccc ctcgtgcgtc cgctgccacg tccccgcggg cacgacgccc ggactcgact
     2701 tcgcaaaggg cacggccgcg cttccgaagg gccgcttcgg gaacggcgtg gactgggagg
     2761 ctgcggaatc gaagggcctc atccacccgc tcgacgcgct cgccggcgtc tccgtcgtcc
     2821 gcaagccgct ccagatcccg cccgactacg acatcgccgc gaagatcaac acgcttcccg
     2881 agatcgtctt ctcgcacaag aagcacgccg tgtggggcgg ctgcgagctc tgccaccccg
     2941 acgtcttcac cgtcaagcgc ggctcgacga agttcacgat ggaggagatc ttcaaggggc
     3001 gttactgcgg cgcctgccac ggcacggtcg cgttcccgac gaccgactgc cagcgctgcc
     3061 acacgaagcc ggtcgcgtga acccggccgt ccccgcgctc tttcttctcc tcgcgggtca
     3121 gccctggttc aagctgcctc ctcaccccag gcccgaggtc ttcggcaacg tcctcatctc
     3181 gcgcacgacc gccgcgggcc ccgtcaagcc cgcggcgttc tcccactgga gccaccggcg
     3241 ccgccatacg tgccgcgtct gccacctcga gctcgagttc tccttcaagc gcaacacgac
     3301 gccgatcacc gaggaggcaa accgccaggg ccgcttctgc ggaggctgcc acgacgggaa
     3361 gacggccttc gcccacaccg agccaaactg cacgaggtgc cactcgggcg acctcgccgc
     3421 gggcgccgtg aagttcggcg agctgcgcga cctgcaggag gcgccgttcg gaaacggggt
     3481 ggactggtcc gcggccctcg agtccggccg catcgccccc gccgcgagcc tcacgaaggg
     3541 cgaaggcggg atgtccctca agtcgaagct ctccctcgcg gccgagtggg ccatggtccc
     3601 gcccgcgatc ttcccgcacg acgagcacac gcgctggctc gactgcgcga actgtcaccc
     3661 cgagcccttc aacatccaga agaagacgac gaagcacttc tcgatggcga agaatctcgc
     3721 cggtgagttc tgcggcgcgt gccacctcac ggtcgccttc ccgctcgacg actgcaagag
     3781 gtgccacccg aagatgaggg cgaattcctg agccgtcggc tggcgtcacg cagcccctgg
     3841 cccgggtcct cgtgctgccg tcgttactcg ttgaagacgt tgttgtggca ctgctggcag
     3901 ccggcggcgc ccgtggcggt cctcgtgccg tagtcccagc ggagcgagaa ggggttggcg
     3961 gaaccgtgcg ccttgtggca cgtgagacag aagacctcgt tgttcgcggc gacgaccgtc
     4021 gcgctcgcga agctcgttgc gccgacggcc gtgaacggca gccgcgccac ggtgaacccc
     4081 gccgtcccgc tcacccagtt cgccgggtcg gtgttcgcgt cagccttgtt gatcgggtaa
     4141 tagccggcgg actccgtgtt cgttcccggg tggcggtgat acggggaagc cgtgttcgtg
     4201 taccacggcg cgaagaacgt gtggtggcag tcgatgcaca tgttcgtgat ctcccgccag
     4261 gcgccgtccg tcgacgacgg cgcgggatag gcgacgttcg cggcctcgta tcgggcgagg
     4321 ccggtcgtgc cgcccttgat cgaggcgagg aggggcggct cggagccggg agcggacgcc
     4381 caccagaggt tccggtagga cgcgttcccg tgcgggttgt ggcagtcgat gcactgcacc
     4441 tcggccgtcg ccatcgtgcc cacgaagtgg caccggttgc aaaggtccga gtcgccgggc
     4501 ttgccgagag acgtcgtcgt caggttgtgg ccgcggtggc tcctcacgcc cgcgggcgcg
     4561 aagtatcccg ccgcccgctc cgtcagcccg ttgacgtcca cgtccacgac gtccggggcg
     4621 aacgtctgcc cctcgtgaca cgacaggcag agcatcgtgg agttcgtgcc cttgagcatg
     4681 tagccgttcg gagcgccgga aggcatccag tcgccgtccg gagcgcctgt ggtgctcacg
     4741 gggccgccgt cgaagccgtg cgtctcgctc ccgtgcgccg cgtggcagtc ggagcagagg
     4801 agcgtcgggc cgttgtggta gtaggccgcg ccgtccgtcg gcgccgcgag agcacgcctg
     4861 ccggccccgg gcgtgcgcgg cgcccggtgg agctggatac ccgtcatgct ctgcgcgaag
     4921 ccgcccggcg cggcgaggac cgcgatcagg gcgccgaggg cgatcgtcga gatggagtgg
     4981 aatcggccgg gacggggggc tcccaagcgt tcggacatgc gtctccttct tcagccggat
     5041 ctatttcacc ttggcgttct cgaggatcac ggggtaggcg taccccgcac cgaaatcctt
     5101 gtccacggac accgcacccg cgatcgtcac gacgtctccg accttgacga cgtcggaggt
     5161 cgtgaccgtc aggtcgtggt ccttcttctg ggccgacccg gacccgtcgc gcacgtgcag
     5221 ccagttcttt cccatgacgc cggacgtcac cttgacgacc tttgccttca cgacgacgtc
     5281 ctggcctctc agcgcggcgc gcgacgccca cacctcggcg accgtcttcg cgttcgcacc
     5341 ctcggccttc gggaccttca cgtccgcggc ctcggcgggg ccggcggcca tctggccgtg
     5401 tcgcgcggcc atctcggcgc gcgtctcg
//
LOCUS       DAOMFY010000271         5402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5263_length_5402_cov_7.878437, whole genome shotgun sequence.
ACCESSION   DAOMFY010000271 DAOMFY010000000
VERSION     DAOMFY010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5263_length_5402_cov_7.878437"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLB01_16485"
     CDS             <1..239
                     /locus_tag="PLB01_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78951.1"
                     /translation="DADAARAALPRWIHASGGLAGFEAAARKAAGLPDDLPLWGRLLL
                     VDARGRIRRVAVPLKPELDQLMRDTGFLVNMEGR"
     gene            240..854
                     /locus_tag="PLB01_16490"
     CDS             240..854
                     /locus_tag="PLB01_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQQ78952.1"
                     /translation="MSAPSEAVLAAPGATPRNAPTFGAATTGKVGMWVFLVTDAMTFA
                     GLLLGYAILRAGNPKWPLPSTVLGIPFTGVMTFVLILSSVTMVQALDAAKHGERRQTA
                     LWLFATALGGAFFLGGQVYEYTHLIRHGFTLPSGNFPATFYVVTSFHGAHVFTGVCYL
                     LITAVGVLRGKFLDNGANVVEITGLFWHFVDLIWILVFTFVYLI"
     gene            866..1225
                     /locus_tag="PLB01_16495"
     CDS             866..1225
                     /locus_tag="PLB01_16495"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQQ78953.1"
                     /translation="MSDHATTHDDSPAAYVRFGPLRMRREYFMVLIVLSVLTVAEIAV
                     ASLKMDRRVMAALLIGMALTKAAFVGLYYMHLKHEKRGLMWMAAIPLPLAGLYAAALM
                     LDGANLLRVATLPWVHH"
     gene            1234..1446
                     /locus_tag="PLB01_16500"
     CDS             1234..1446
                     /locus_tag="PLB01_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78954.1"
                     /translation="MTETLRAAALAVLAGGAVLLAPAAAACPSCSASSSPKEPNVWPV
                     VGAFMLVPWVLAAGVAVIVRRNSRSA"
     gene            1492..4263
                     /locus_tag="PLB01_16505"
     CDS             1492..4263
                     /locus_tag="PLB01_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ78955.1"
                     /translation="MPHARRAAAGAVLALALALAGALALPLAAADAPKSTAPEKITSV
                     EGITEYRLANGLRVLLFPDPSKPTITVNVTYLVGSRNEGYGETGMAHLLEHLVFKGTP
                     RHPNIPQELTAHGTRPNGTTSFDRTNYFETFDATDANLEWALDLEADRMVNSFIAKKD
                     LDSEMTVVRNEFEAGENQPSVILFKRMLASAFHEHGYGHLPIGSRSDIENVPIERLQA
                     FYRNYYQPDNAVLLVAGKFDEKKTLDLVQKFFGAIPRPARTLYPTYTLDPPQDGERAV
                     TIRRVGDVQLVAAAYHIPSGSDADAPGLDLLAFALADTPSGRLHRSLVEAKKASSIFG
                     FAWGLKEPGLAIFGAELRQDQNLDEAREILLKTVESAAANPPAKEEVERARTQLVKQI
                     DLALNDSSQVGLRLSEWIAKGDWRLFFLYRDRLKAYPVEGVARVAAAYLKPTNRTLAT
                     FIPTAKPDKAEVPPPADVAAMLKGYTGAAAVAAGEAFDPSPANIETRTRRSTLPSGMK
                     VALLPKKTRGASVRGTIVLHFRDLASLTGKSAVSDLTADMLERGTAKKTRQEIQDTID
                     RLKARIRVAGGGSRTAVSIETTRENLPKVLDLVAEILKEPSFPASELEILRQENLANI
                     DQMKTEPQAVAGTAFGRHMRPYPKDDPRYTPTPDESLAAYKAATLDAVKAFWKESYGA
                     SNAEAVLVGDFDEAAVHAQLSALFGAWKSPSPYTRVPDLYRDVPADSLRLETPDKANA
                     VFFAGQNLPLKDEDPDYPALALANHMFGGGFLNSRLAVRIRQKDGLSYGVGSSLDPGT
                     LDASGSFTVFAIHAPQNTARLEAAVREEIERARKEGFTAEELAAARSGWLQGRQVSRS
                     QDGELAGLLQRRVFQGRTIAWDAELEKKVSALAADQVTAAFRKWIDPAKVSVVRAGDF
                     ANVKADAKK"
     gene            4275..5306
                     /locus_tag="PLB01_16510"
     CDS             4275..5306
                     /locus_tag="PLB01_16510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ78956.1"
                     /translation="MQPVDDRGREVARLLEGRYEVLGLLGRGGFAAVYQVRNLRLQRL
                     EALKVVAGTHEESSEFRQRFEQEARVAASLEHPSILKVYDFGHDAGMLWYSMPFVDGP
                     TLKAELARRGTFDEEAAARMAVSLLDALEYSHGRGVIHRDIKPDNILLDRRDRPYLMD
                     FGIAKTDDSLVKTQTGMVLGSPAYISPEQLRGERLDGRTDVYSLGATLFQAVAGTVPF
                     RSDRMADLAERLVSDAPRLAARKPGVSPEFDRIVARALARLRDERYANARQMRQDLEA
                     FLDSLRRAPAVPAADPASLEDTATLSAVALPPIPVPAPPPVLETPPAREPAPRPAPRG
                     GARCRGSPA"
BASE COUNT          830 a         2029 c         1769 g          774 t
ORIGIN      
        1 gcgacgcgga cgcggcccgc gccgcgctcc cccgctggat ccacgcgagc ggcggcctcg
       61 ccggcttcga ggccgcggcc cggaaggcgg cgggccttcc cgacgacctg cccctgtggg
      121 gccgtctcct cctcgtcgac gcacgcggcc ggatccgccg cgtcgccgtg cccctcaagc
      181 cggagctcga ccagctcatg cgcgacactg gattcctcgt gaacatggaa ggacgttgaa
      241 tgagcgcacc gtctgaggcc gtcctcgccg caccgggagc cacgccccgc aacgcgccga
      301 ccttcggcgc cgccacgacc gggaaggtcg ggatgtgggt cttcctcgtc acggacgcga
      361 tgacgttcgc gggcctgctc ctcgggtacg cgattctccg cgcgggcaac ccgaagtggc
      421 ccctgccgtc gaccgtcctc gggatcccgt tcacgggcgt catgacgttc gtcctgatcc
      481 tctcgagcgt cacgatggtc caggcgctgg acgcggccaa gcacggcgaa cgccggcaga
      541 ccgccctctg gctctttgcg accgcgctcg gcggcgcgtt cttcctcggc gggcaggtct
      601 acgagtacac gcacctcatc cggcacggct tcacgctgcc ctccgggaac ttccccgcga
      661 ccttctacgt cgtcacgagc ttccacggcg cgcacgtctt caccggcgtc tgctacctcc
      721 tgatcacggc ggtcggcgtc ctgcgcggga agttcctcga caacggcgcg aacgtcgtcg
      781 agatcacggg cctgttctgg cacttcgtcg acctgatctg gatcctcgtc ttcacgttcg
      841 tctacctcat ctgacgggag cgcccatgag cgaccacgcc acgacgcacg acgattcccc
      901 ggccgcctac gtccgcttcg gtcccctcag gatgcggcgc gagtacttca tggtcctcat
      961 cgtcctttcc gtgctgacgg tcgcggagat cgccgtcgcg agcctgaaga tggaccgccg
     1021 cgtcatggcg gccctgctga tcgggatggc cctcacgaag gccgcattcg tgggcctcta
     1081 ctacatgcac ctcaagcacg agaagcgcgg gctcatgtgg atggccgcga tcccgctgcc
     1141 gctcgccggc ctctacgccg ccgccctcat gctcgacggc gcgaacctgc tccgcgtcgc
     1201 gacgctgccg tgggtccacc actgatgcgc gccgtgacgg aaacgctccg cgccgccgcc
     1261 ctcgccgtcc tcgcgggagg cgccgtcctg ctggcgccgg cggccgccgc gtgcccatcc
     1321 tgctccgcga gctcgtcgcc gaaggagccg aacgtctggc cggtcgtcgg cgcgttcatg
     1381 ctcgttccgt gggtcctcgc ggccggcgtc gcggtgatcg tccgccgcaa ctcccggtcg
     1441 gcatagactt ccgcccgtgc gggcgcctcc gtgcgcccct gaggagtctt catgccgcac
     1501 gcccgacgcg ccgcagccgg cgccgttctc gccctcgccc tcgccctcgc cggggccctc
     1561 gccctcccgc tcgcggccgc cgacgccccg aagagcacgg cgccggagaa gatcacgtcc
     1621 gtcgagggca tcacggagta ccgcctcgcg aacggcctgc gcgtgctcct gtttccggac
     1681 ccgtcgaagc ccacgatcac cgtgaacgtc acgtacctcg tcgggtcgcg caacgaaggc
     1741 tacggggaga cgggcatggc ccatctcctc gagcacctcg tcttcaaggg cacgccgagg
     1801 cacccgaaca tcccgcagga gctcacggcc cacgggacgc gtccgaacgg gacgacctcc
     1861 ttcgaccgga cgaactactt cgagacgttc gacgcgaccg acgcgaacct cgagtgggcg
     1921 ctcgacctcg aggccgaccg gatggtgaac tcgttcatcg cgaagaagga cctcgacagc
     1981 gagatgaccg tcgtccgcaa cgagttcgag gccggcgaga accagccgag cgtgattctc
     2041 ttcaagcgca tgctcgcgtc ggccttccac gagcacggct acgggcacct gccgatcggc
     2101 tcgcgctcgg acatcgaaaa cgtgccgatc gagcgcctcc aggccttcta ccggaactac
     2161 taccagcccg acaacgcggt cctcctcgtg gccgggaagt tcgacgagaa gaagacgctc
     2221 gacctcgtcc agaagttctt cggcgcgatc ccgaggcccg cccggacgct gtacccgacg
     2281 tacacgctcg atccgccgca ggacggggag cgggcggtga cgatacgccg cgtcggggac
     2341 gtccagctcg tcgcggccgc gtaccacatc ccgtccggca gcgatgcgga cgcgcccggc
     2401 ctcgacctcc tcgcgttcgc cctcgcggac acgccctccg gccgcctgca caggagcctc
     2461 gtcgaggcca agaaggcctc gtcgatcttc ggcttcgcgt gggggctcaa ggagcccggc
     2521 ctcgcgatct tcggcgccga gctccggcag gatcagaacc tggacgaggc gcgcgagatc
     2581 ctgttgaaga ccgtcgagag cgccgccgcg aacccgccgg cgaaggagga agtcgagcgc
     2641 gcgcggacac agctcgtgaa gcagatcgac ctcgccctca acgactcctc gcaggtgggc
     2701 ctgcgtctct cggagtggat cgcgaagggc gactggcggc tcttcttcct ctatcgggac
     2761 cgcctcaagg cctaccccgt ggagggcgtc gcccgggtcg ccgcggctta cctgaagccg
     2821 acgaaccgta cgctcgccac gttcatcccg accgcgaagc ccgacaaggc cgaggtgccg
     2881 ccgcccgccg acgtcgcggc aatgctgaaa ggctacacgg gcgcggccgc cgtcgcggcc
     2941 ggcgaagcgt tcgatccgtc gcccgcgaac atcgagaccc ggacgaggcg gtccacgctg
     3001 ccttccggga tgaaggtcgc cctgctcccc aagaagacgc gcggcgcgtc cgtccgcggc
     3061 acgatcgttc tccacttccg cgacctcgcc tccctcacgg gcaagtcggc ggtctcggac
     3121 ctgaccgccg acatgctcga acgcggaacc gcgaaaaaga cgcgtcagga aatccaggac
     3181 acgatcgacc ggctcaaggc gcggatccgg gtcgccggcg gaggcagcag gacggcggtc
     3241 tcgatcgaga cgacgcgcga gaacctcccg aaggtcctcg acctcgtcgc cgagatcctc
     3301 aaggaaccgt ctttcccggc gtccgaactc gagatccttc gccaggagaa cctcgcgaac
     3361 atcgaccaga tgaagaccga gccgcaggcg gtcgccggga ccgcgttcgg gcgccacatg
     3421 cgtccctacc cgaaggacga tccgcggtac acgccgacgc cggacgagag cctcgccgcg
     3481 tacaaggcgg cgaccctcga cgccgtgaag gcgttctgga aggagtcgta cggcgcgtcg
     3541 aacgcggagg ccgtcctcgt gggcgacttc gacgaggccg ccgtccacgc gcagctttcg
     3601 gccctcttcg gggcgtggaa gagcccgagc ccgtacaccc gcgtcccgga cctctaccgt
     3661 gacgtccccg ccgactcgct ccggctcgag acgcccgaca aggcgaacgc cgtcttcttc
     3721 gcgggacaga acctcccgct caaggacgaa gaccccgact atccggcgct cgcgctcgcg
     3781 aaccacatgt tcgggggcgg cttcctgaac tcgcgcctcg ccgtccggat ccggcagaaa
     3841 gacggcctct cgtacggcgt cggctcgtcg ctcgacccgg gcacgctcga cgcctccggg
     3901 tccttcacgg tcttcgcgat ccacgcgcca cagaacaccg caagactcga agctgccgtg
     3961 cgtgaggaaa tcgagcgtgc ccgcaaggaa ggcttcacgg ccgaggagct cgcggccgcg
     4021 cgctcgggct ggctgcaggg ccggcaggtg agccgctcgc aggatggcga gctcgccggc
     4081 ctcctccaga ggcgcgtctt ccagggacgc acgatcgcct gggacgccga gctcgagaag
     4141 aaggtctccg ccctcgcggc ggatcaggtg actgccgcgt tccgcaagtg gatcgacccg
     4201 gcgaaggtct cggtcgtccg cgcgggcgac ttcgcgaacg tgaaagcgga cgccaagaaa
     4261 tgaagacctg acggatgcag cccgtcgacg acaggggccg ggaagtcgcc cggctcctcg
     4321 aaggccgcta cgaggttctc gggctgctcg gccgcggcgg cttcgccgcg gtctaccagg
     4381 tccggaacct gcgtctccag cggctcgagg ccctgaaggt cgtcgccggg acgcacgagg
     4441 agagctcgga attccggcag cgcttcgagc aggaggcccg cgtcgcggcg tcgctcgagc
     4501 acccgagcat cctgaaggtc tacgacttcg ggcacgacgc gggaatgctc tggtactcga
     4561 tgccgttcgt ggacgggccg accctcaagg ccgagctcgc gcggcgcggg accttcgacg
     4621 aagaggcggc cgcccgcatg gccgtctccc tgctcgacgc actcgagtac agccacgggc
     4681 gcggcgtgat ccaccgcgac atcaagccgg acaacatcct gctcgaccgg cgcgaccggc
     4741 cgtacctcat ggacttcggc atcgcgaaga cggacgacag cctcgtgaag acgcagacgg
     4801 ggatggtcct cggctcgccc gcgtacatct ctcccgagca gctccgcggc gagcggctcg
     4861 acgggcgaac ggacgtgtac tcgctcgggg cgaccctctt ccaggcggtc gccggcaccg
     4921 tgccgttccg ctcggaccgg atggccgacc tcgcggaacg cctcgtgtcg gacgctcccc
     4981 gcctcgccgc gcgcaaaccg ggcgtgagcc ccgagttcga ccgcatcgtg gcccgcgcgc
     5041 tcgcccgcct ccgcgacgag cgctacgcaa acgcgcgcca gatgcgccag gacctcgagg
     5101 cgttcctcga ctccctccgc cgggctccgg cggttcccgc cgccgatccc gcgtcgctcg
     5161 aggacacggc gaccctctcg gccgtggccc tgccgccgat ccccgtcccg gcgccgccgc
     5221 ccgttctgga gacaccgccc gcgcgcgagc cggccccgcg tcccgcgccg cgcggaggtg
     5281 ctcggtgccg tggctctcca gcgtgaagag gtcgccgcgg cccttgagca ggaagaagcg
     5341 gtcgagcgcg cagcgggcga aggtgcgaac gtggtggtac gcgtcccaga agccgtcggg
     5401 cg
//
LOCUS       DAOMFY010000272         5381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5285_length_5381_cov_6.530605, whole genome shotgun sequence.
ACCESSION   DAOMFY010000272 DAOMFY010000000
VERSION     DAOMFY010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5285_length_5381_cov_6.530605"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLB01_16515"
     CDS             complement(<1..942)
                     /locus_tag="PLB01_16515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ78957.1"
                     /translation="MAAADVLPRSIGRYEIVRLLGKGGMGEVFLATDPALGRRVAIKV
                     LPAALQQDAKMRGRFVNEARAVAALNHPNVITLHDLAVTDARDAGFPPGALYLVLEYL
                     EGKGLDEIMDARRLGVGECVEIAIQVLEGLKAAHQSRILHRDITPANVMLAPDGRAKL
                     LDFGLSKILLEGTRDAARAARHTGEGMIVGTLDYLSPEQALGGELDERSDLFSFGIVF
                     YQMLAGVHPFSGASVTQMVAKMMTKEADPWPEPDLVPDVVKQIVARALKKDASERYGS
                     AGQMILDLLVARRELAARPARGAVTREVPAAVPPPAIR"
     gene            complement(969..1535)
                     /locus_tag="PLB01_16520"
     CDS             complement(969..1535)
                     /locus_tag="PLB01_16520"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ78958.1"
                     /translation="MVRSVREIPLGAVAPYPEADASDATLKDGTRVHLRPIRQDEGPH
                     LLELYDRLSPESLYFRFFAVPDKDRSKAEYLARVDYANRYALVAEVAGVLVGVARWER
                     DVSAPSHAEVAFTVADAQQGKGLGSILFRRLATLARARGITTFEAEVLPSNEKMLRVF
                     ERSGLPQTSRRDSPALHISIALGDEPAA"
     gene            1606..2004
                     /locus_tag="PLB01_16525"
     CDS             1606..2004
                     /locus_tag="PLB01_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQQ78959.1"
                     /translation="MKLITTIIRPEKLADVKAALFRAGVTGISLSRVQGHGGEHDTVE
                     QYRGTTLVFEFVEKVRIDIAVSEPFVEPTIEAIVAAARTGEVGDGKIFVQPLERVIRI
                     RTGEQDVQALTPISAEEVRAKALRAGKEGA"
     gene            2001..3296
                     /locus_tag="PLB01_16530"
     CDS             2001..3296
                     /locus_tag="PLB01_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQQ78960.1"
                     /translation="MNAPAVSAGDTAWVLVAAALVFVMNPGLAFFYAGLVRRKNTLNT
                     LMMTYVAMGVGAVVWMAAGYSLAFAPGSPFLGGLRWLGLSGVGGTPEPDYAATIPALA
                     FFAFQGMFATITPALVSGAIVERMSFRAYVIFLAAWSLVVYAPVAHWVWGTGGFLRAW
                     GALDFAGGTVVHVTAGTAALVAARMLGPRQDFKRIALIPHNVPYVLLGAGLLWFGWFG
                     FNAGSALAANGTASLAFVTTNAGAAAAALTWLALESRFGGHATAVGAATGAVVGLVGI
                     TPGAGFVSPLSALAIGALTASASFTALRLRGRTSLDDTLDVFACHGIGGITGSLLTGV
                     FASKAANPDGGNGLLFGNAALFGIQAATVGVVFVYTLLMTALVLLAIRAVTPLRVALH
                     AELDGLDRRVHGEEAYRGGSAAGGLGESVILHPAERGKT"
     gene            complement(3293..4798)
                     /gene="malQ"
                     /locus_tag="PLB01_16535"
     CDS             complement(3293..4798)
                     /gene="malQ"
                     /locus_tag="PLB01_16535"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQQ78961.1"
                     /translation="MTNRTREAGILLHPTSLPSRFGIGDLGPGADSFLDWAGSAGLRI
                     WQVLPLGPVGAHDSPYAGLSAFAGNPLLVSPEWLLEEGLLPAAALDGAPDFPANRVDF
                     AAVRPFKERILRASFDHARSIGSSVTAAVERFEASPSQSSWLPDWALFAALRAAHGDA
                     GWTEWPAALVSREEGALDAARKEHADEIAFHVFAQFLFFRQWERVKRAANARGISILG
                     DLPIYVALDSADVWSQRRLFAVDAKGRPERVAGVPPDDFSDDGQLWGHPLYRWDVLAE
                     ESFDWWIERVRANLRLADLVRLDHFRGFAGYWEVPAGAASAREGRWATGPGLMLFLAI
                     RHALGDVPIIAEDLGEITDDVRQLLATLGVPGMKVLQFAFGEMDSEHLPHHHVPNAVV
                     YSATHDNDTAAGWYAAAPDWAQERLRDYFGVDGKDPAGVLVRAAYASVADRAVVPLQD
                     VLGLGSEARMNVPGRAHDNWTWRAPKDAFRPEVAARLLRLAELTGRVAAAR"
     gene            complement(4798..>5381)
                     /locus_tag="PLB01_16540"
     CDS             complement(4798..>5381)
                     /locus_tag="PLB01_16540"
                     /inference="COORDINATES: protein motif:HMM:NF037725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HQQ78962.1"
                     /translation="APDQVLGGPRGQVFLGCLPPAADGYRREITQAAMKIGAVLAAHG
                     VVSRFAVDFLAVKDRPDEPWRLSALEINLRMGGTTHPYLALQFLTGGSLDPESGLFLS
                     PRGSPKFYRATDNLQSEAYRGLLPEDLIEIVTINALHYSHNSESGVLFHLIGALSQYG
                     KLGMTAIGNSREEVEALYDKTLRILDRETAIGH"
BASE COUNT          751 a         1864 c         1995 g          771 t
ORIGIN      
        1 ccggatcgcc gggggcggga cggctgccgg gacctcgcgg gtcacggcgc cgcgggcggg
       61 ccgcgccgcg agctcgcgcc gcgccacgag gaggtcgagg atcatctgcc ccgcgctccc
      121 gtaccgctcg gacgcgtcct tcttgagcgc gcgcgcgacg atctgcttga cgacgtcggg
      181 cacgagatcc ggctcgggcc acgggtcggc ctccttcgtc atcatcttgg cgaccatctg
      241 cgtcacgctc gcgcccgaga acgggtgcac gccggcgagc atctggtaga agacgatccc
      301 gaaagagaag aggtccgagc gctcgtcgag ctctccgccg agcgcctgct cgggggagag
      361 gtagtcgagc gttccgacga tcatcccttc gcccgtgtgc cgcgccgccc gggccgcgtc
      421 gcgcgtgccc tcgaggagga tcttgctgag tccgaagtcg aggagcttgg cgcggccgtc
      481 gggtgcgagc atgacgttcg cgggcgtgat gtcccggtgg aggatccggc tctggtgcgc
      541 ggccttgagg ccttcgagga cctggatcgc gatctcgacg cactcgccga cgccgagccg
      601 gcgcgcgtcc atgatctcgt cgaggccctt gccctcgagg tactcgagga cgaggtagag
      661 cgccccgggc ggaaaacccg cgtcccgggc gtccgtgacg gcgaggtcgt gcagcgtgat
      721 gacgttcggg tggttgaggg ccgcgacggc cctcgcttcg ttcacgaagc gcccgcgcat
      781 cttcgcgtcc tgctggaggg cggccggaag gaccttgatc gcgacgcggc ggccgagcgc
      841 gggatccgtc gcgaggaaca cctcccccat cccgcccttt ccgagcaggc ggacgatctc
      901 gtacctgccg atgctgcggg ggagaacgtc cgctgcggcc actcggaggt caagtatagg
      961 cgggcgtgtc aggccgccgg ctcgtccccg agggcgatgg agatgtgcag tgccggggag
     1021 tcgcgccggg aagtctgggg aagcccggac cgctcgaaga cgcggagcat cttctcgttg
     1081 ctcggcagga cttccgcctc gaaggtcgtg attcctcggg cgcgggcgag cgtcgcgagc
     1141 cgccggaaca ggatcgaccc gaggcccttc ccctgctggg cgtccgcgac cgtgaacgcg
     1201 acctcggcgt gcgacggcgc ggaaacgtcg cgctcccagc gcgcgacgcc gacgaggact
     1261 ccggcgacct cggcgacgag cgcgtagcgg ttggcgtagt cgacgcgggc gaggtactcg
     1321 gccttcgaac ggtccttgtc gggcacggcg aagaaccgga agtagaggct ctcgggggag
     1381 aggcggtcgt agagctcgag gaggtgcgga ccctcgtcct gccggatcgg ccgcaggtgg
     1441 acgcgcgtgc cgtccttcag cgttgcgtcc gacgcgtccg cctcggggta cggggcgacg
     1501 gcccccagcg ggatctcccg gaccgatcga accaacgcag acctccgagg accctacatg
     1561 atggtacaga atcgcgcttc aagtccaagg tggggagaaa gcgccatgaa gctcatcacg
     1621 accatcatcc ggccggagaa gctcgcggac gtgaaggccg cgctcttccg ggcgggcgtc
     1681 acgggcatct cgctctcgcg cgtccagggg cacggcggcg agcacgacac ggtggagcag
     1741 taccggggga cgacgctcgt cttcgagttt gtcgagaagg tccggatcga catcgcggtc
     1801 tccgagccgt tcgtcgagcc gacgatcgag gcgatcgtcg cggcggcgcg cacgggggag
     1861 gtcggcgacg ggaagatctt cgtccagccg ctcgagcgcg tgatccggat ccgcacgggc
     1921 gagcaggacg tgcaggccct gacgccgatc tcggccgagg aagtgagggc caaggcgctg
     1981 cgcgccggca aggagggcgc gtgaacgctc cggccgtcag cgccggcgac accgcctggg
     2041 tcctcgtcgc cgccgcgctc gtcttcgtca tgaaccccgg gctggcgttc ttctacgccg
     2101 ggctcgtgcg gcggaagaac acgctgaaca ccctcatgat gacgtacgtc gcgatgggcg
     2161 tcggcgccgt cgtctggatg gccgcgggct actcgctcgc cttcgcgccc ggctcgccgt
     2221 tcctcggcgg cctgaggtgg ctcggcctct cgggagtcgg cggcacgccc gagccggact
     2281 acgcggcgac gatcccggcg ctcgcgttct tcgcgttcca ggggatgttc gcgacgatca
     2341 cgcccgcgct cgtgtcgggc gcgatcgtgg agcggatgag cttccgcgcc tacgtcatct
     2401 tcctcgcggc ctggagtctc gtcgtctacg ccccggtcgc gcactgggtg tgggggacgg
     2461 gcgggttcct gagggcctgg ggcgcgctcg acttcgcggg gggcacggtc gtgcacgtga
     2521 cggcgggaac ggcggcgctc gtcgccgcga ggatgctcgg gccccgccag gacttcaaga
     2581 ggatcgccct catcccgcac aacgtcccgt acgtcctcct cggtgcgggc ctcctgtggt
     2641 tcgggtggtt cgggttcaac gcaggctcgg cgctcgcggc caacgggacc gcgtcgctcg
     2701 cgttcgtgac caccaacgcg ggagcggccg ccgccgcgct gacgtggctc gcgctcgaga
     2761 gccggttcgg cgggcacgcg accgccgtcg gcgccgcgac gggcgccgtc gtcggtctcg
     2821 tcgggatcac cccgggcgcc ggattcgtct cgccgctctc ggctctcgcg atcggcgccc
     2881 tgacggcctc cgcgagcttc acggcgctgc gcctgcgcgg ccgaacgtcc ctcgacgaca
     2941 cgctcgacgt cttcgcctgc cacggcatcg gcgggatcac gggctcgctc ctgacgggcg
     3001 tgttcgcgtc gaaggccgcc aaccccgacg gcggcaacgg cctcctcttc gggaacgccg
     3061 ccctcttcgg gatccaggcc gcgacggtcg gcgtcgtgtt cgtctacacg cttctcatga
     3121 cggccctcgt gctcctcgcg atccgggccg tgacgccgct gcgcgtcgcc ctccacgccg
     3181 agctggacgg cctggaccgc cgcgtccacg gcgaagaggc ctatcgcggc ggcagcgcgg
     3241 cgggaggcct cggcgagagc gtgatcctgc acccggccga gcgcggaaag acctagcgcg
     3301 cagccgcgac gcggcccgtc agctccgcga gacggagcag gcgggccgcg acttcgggcc
     3361 ggaacgcgtc cttcggagcg cgccaggtcc agttgtcgtg ggcgcgcccc gggacgttca
     3421 tccgcgcctc ggacccgagg ccgagcacgt cctggagcgg aacgacggcg cggtccgcga
     3481 cggaggcgta ggccgcgcgg acgaggacgc cggccgggtc tttcccgtcc acgccgaagt
     3541 agtcccgcag acgctcctgc gcccagtccg gcgccgccgc gtaccagccc gcggcggtgt
     3601 cgttgtcgtg tgtggcggag tagacgacgg cgttcgggac gtggtggtgc ggcaggtgct
     3661 cgctgtccat ctctccgaac gcgaactgga ggaccttcat cccgggcacg ccgagcgtcg
     3721 cgaggagctg gcgcacgtcg tccgtgatct ctccgagatc ctccgcgatg atcggcacgt
     3781 cgccgagcgc atggcggatc gcgaggaaca gcatgaggcc gggccccgtc gcccaccggc
     3841 cctcgcgcgc ggacgccgcg ccggccggca cctcccagta tcccgcgaag ccgcggaagt
     3901 ggtcgaggcg caccaggtct gcgaggcgga ggttcgcgcg gacgcgctcg atccaccagt
     3961 cgaaggactc ctcggcgagg acgtcccacc ggtagagcgg gtgcccccag agctgcccgt
     4021 cgtcgctgaa gtcgtccggc gggacgccgg cgactctctc gggccggccc ttcgcgtcca
     4081 cggcgaagag ccgccgctgg ctccacacgt cggcgctgtc gagtgcgacg tagatcggga
     4141 ggtcgccgag gatcgaaatg ccgcgcgcgt tggcggcgcg cttgacgcgc tcccactgcc
     4201 ggaagaacag gaactgcgcg aacacgtgga atgcgatctc gtccgcgtgc tccttccgcg
     4261 ctgcatcgag cgcgccctcc tcgcgggaga cgagcgcggc cggccactcc gtccaccccg
     4321 catcgccgtg cgccgcgcgg agggccgcga agagggccca gtcggggagc cacgaagact
     4381 gcgagggaga cgcttcgaaa cgttccaccg cggccgtcac gctcgagccg atcgaccgcg
     4441 cgtgatcgaa cgaggcgcgc aggatgcgct ccttgaacgg ccgcaccgcc gcgaagtcga
     4501 cgcggttcgc cggaaagtcg ggtgcgccgt cgagcgcggc ggccgggagg agcccctcct
     4561 cgagcagcca ctcgggcgag acgagcagcg ggttgcccgc gaaggcggag aggcccgcgt
     4621 acggggaatc gtgtgcgccc accgggccga gcgggaggac ctgccagatc cggaggccgg
     4681 cggacccggc ccagtcgaga aacgagtcgg cgccgggacc cagatcgccg atcccgaaac
     4741 gggagggcag cgacgtcggg tgcaggagga tcccggcctc gcgcgtccga ttcgtcatca
     4801 atggccgatc gccgtttcgc ggtcgaggat ccggagcgtc ttgtcgtaga gcgcctcgac
     4861 ctcctcgcgg ctgttgccga tcgccgtcat cccgagcttg ccgtactggg acagcgcgcc
     4921 gatgaggtgg aagagcacgc ccgactcgga gttgtggctg tagtggagcg cgttgatcgt
     4981 gacgatctcg atcagatcct ccggcagcag cccgcggtac gcctcggact ggaggttgtc
     5041 cgtggcgcgg tagaacttcg gcgacccgcg cggcgagagg aagagccccg actcggggtc
     5101 gaggctcccg cccgtgagga actggagcgc gaggtagggg tgcgtcgtgc cgcccattcg
     5161 gaggttgatc tcgagcgccg aaaggcgcca cggctcgtcc gggcggtcct tcacggcgag
     5221 gaagtcgact gcgaaacggc tcacgacgcc gtgcgccgcg agcacggcgc cgatcttcat
     5281 cgcggcctgc gtgatctccc ggcggtagcc gtcggcggcg ggggggaggc acccgaggaa
     5341 gacctggccg cgcggcccgc cgaggacctg gtcgggcgcc g
//
LOCUS       DAOMFY010000273         5377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5292_length_5377_cov_5.921458, whole genome shotgun sequence.
ACCESSION   DAOMFY010000273 DAOMFY010000000
VERSION     DAOMFY010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5292_length_5377_cov_5.921458"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLB01_16545"
     CDS             complement(<1..972)
                     /locus_tag="PLB01_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78963.1"
                     /translation="MKAVLRGALAALLSFLLAAGSAGACCTDLWSCAGAVASAGLTCA
                     EEAAALALRDLARRLTAERDARKQAFEAAVAAETGQANDVSSAWRRSAEDAAADAARS
                     AKRAAEITGNDTRLRLLASSGVSAPSARPTPTPAASRFGALDTTMKARPIGADPTLEE
                     LQKRVESEKREVEARRQRAAEQDATARRAIEEAKVWQVAAFTGGVLAGFASLLTQIAI
                     LLGSNDKLKQFVLAANVAGLVVEFVKLEKGFEKDLADAAREAARRRAEAADRPRPDAL
                     AAEQHAKRARDLVAAMERISRIQSLEQRPQTARELVLAPTGGPAERAP"
     gene            complement(1017..2261)
                     /locus_tag="PLB01_16550"
     CDS             complement(1017..2261)
                     /locus_tag="PLB01_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQQ78964.1"
                     /translation="MPPDQGSGERFRPFPAPIGKKLGLVCAGGGVTGAIYEIGALAAL
                     EDRLENASITDFDVFVGVSAGAYISALLANGVTPSLLFRNATRSAEARTDVDDLNLFR
                     VNFEEIASRLAKAPFTLLDAAWDYYKNRHETTLTDLVQAFGQLLPSGIFHNEGLEAWM
                     RDWLTRDGRTNDFRKLAKTLRIVAVDLDSGNTAAFGCEGHDDVPISQAVAASCAIPGL
                     YRPVKIKGVDYIDGGVKKTAHISLSIRERCGLTICVNPLVPVRFRPADTNGAGAGALR
                     RRGLPTILDQVFRVTLHSRLKYGLGRYRREAPAADVVVFEPRPEDLPRYMRNIMRTSG
                     RIRIAEYAYRSTMATLDADFKRLSKVFAAHGLILRPACTRDRARVKTHADRASDIIPT
                     RLAASLATLEADLERMTAARGR"
     gene            complement(2358..2852)
                     /locus_tag="PLB01_16555"
     CDS             complement(2358..2852)
                     /locus_tag="PLB01_16555"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HQQ78965.1"
                     /translation="MRTTTRKPASKLPFEVPAPVQKALKNAGTELDRLGAKAKTAAVK
                     AEKKVRVAARDLAKKSETIRKDPRAFAMDVVESGKDLGEKLTKDVKTRVQKAGEQAAR
                     RAEDVTKMVGDKVGSAVEMGLHRLNVPTRHELRSLSAKVDTLGKKIDTLKSRKPAAKG
                     RRAR"
     gene            complement(2849..3544)
                     /locus_tag="PLB01_16560"
     CDS             complement(2849..3544)
                     /locus_tag="PLB01_16560"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HQQ78966.1"
                     /translation="MSDVRRIVRYRNRKLYEARERRFVTLEDLARAVSTGTRIEVIAA
                     DTGEDITARVLSRALASGKGPVAASADTLARILRAGSEAAETVAGAAERVGATSVAES
                     LRNAARPEKLGQTLAPLTRRFEDARQDVEKIVGGLVEKGRVTWEEGARLRDDVGLVFR
                     ESLADLTGSVRDLGGRLGGQPELAREVAAIKTRLAQLESLAQKSFPSNPRERAAAKPG
                     RASAPAARKRRAR"
     gene            3632..5329
                     /gene="hutU"
                     /locus_tag="PLB01_16565"
     CDS             3632..5329
                     /gene="hutU"
                     /locus_tag="PLB01_16565"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQQ78967.1"
                     /translation="MSAPASAPPYAPPALPRGTVLACRSWLTEAPYRMLLNNLDPEVA
                     ERREDLVVYGGSGKAARNPECLEAILRELRRLGDDETLLVQSGKPVGVFRTHPDAPRV
                     LIANSNLVPKWGTWDEFRRLEALGLTMYGQMTAGSWIYIGTQGILQGTYETFAEAARR
                     DLGSPDGSLAGRLAVTAGLGGMGGAQPLAVTMCGGTALVAEVDAARIDKRLATRYLDL
                     RIDGLDAAIDGALAARDAKEAVSIGVRANAVDLLERLLKRGIVPDVLTDQTSAHDELN
                     GYVPHALEGISLSFDDALRLRREDADRYRRAAVSSMGAHVAAMLELKRRGAVTFDYGN
                     NLRAGAAQAGVANAYDIAGFVPLYIRPLFCEGKGPFRWAALSGDPADIDETDRAVLEL
                     FPENAALRRWITLASERVAFQGLPARICWLGYGERAKAGRAFNELVRSGRVKAPIVIG
                     RDHLDCGSVASPNRETEGMKDGSDAIADWPLLNAMLNTAAGATWVSIHHGGGVGIGYS
                     LHAGMVVVADGTDAAAVRLERVLTTDPGMGVIRHADAGYPEAVACAEANGIPVPMRR"
BASE COUNT          723 a         1795 c         2003 g          856 t
ORIGIN      
        1 cggcgcgcgc tccgcgggcc cgcccgtcgg cgcgaggacg agctcgcgag cggtctgagg
       61 gcgctgctcg agcgactgga tgcgcgagat gcgctccatg gcggcgacga ggtcgcgcgc
      121 ccgcttcgcg tgctgttccg ccgcgagtgc gtcgggcctc gggcggtccg cggcctccgc
      181 gcgtcgcctc gcggcttcgc gcgcggcgtc ggcgaggtcc ttctcgaacc ccttctcgag
      241 cttgacgaac tccacgacga ggcccgcgac gttcgcggcc aggacgaatt gcttgagctt
      301 gtcgttcgag ccgagaagga tcgcgatctg ggtgaggagc gatgcgaacc ctgcgagcac
      361 gcccccggtg aaggccgcga cctgccagac ctttgcctcc tcgatggccc tgcgtgccgt
      421 ggcgtcctgc tcggccgccc tctgccggcg ggcctccact tcgcgtttct cgctctcgac
      481 gcgcttctgg agctcctcga gcgtcgggtc cgctccgatg ggccgcgcct tcatcgtcgt
      541 gtcgagagcg ccgaaccggc tcgcagccgg cgtgggcgtc ggccgggccg agggcgccga
      601 cacgcccgac gaggccagga gcctgagtcg cgtgtcgttt ccggtgattt cggcggctct
      661 cttcgccgag cgcgccgcgt ctgcggcggc atcctcggcc gaccggcgcc acgccgacga
      721 gacgtcgttc gcctgcccgg tttccgcggc gacggcagcc tcgaacgcct gtttccgcgc
      781 gtcgcgctcc gcggtgaggc gcctggcgag gtcccggaga gccagggcgg cggcttcttc
      841 ggcgcacgtc aggccggcgc ttgcaaccgc gcccgcgcag ctccacaagt ccgtgcagca
      901 cgcgccggcg gagccggccg cgaggaggaa cgagagaagc gccgcgaggg cgccgcgcag
      961 gaccgccttc atggaatttc ccggagaaaa agagtatcag aaagtgcgac cgcttctcaa
     1021 cggccgcggg cggcggtcat gcgctcgagg tcggcctcca gcgtcgcgag cgacgccgcg
     1081 agtcgcgtcg ggataatgtc gctcgctcgg tcggcgtggg tcttcacgcg ggcgcggtcg
     1141 cgtgtgcagg ccggcctcag gatcaggccg tgggcggcga agaccttcga gagccgcttg
     1201 aagtcggcgt cgagcgtggc catcgtcgaa cggtacgcgt actcggcgat ccggatgcgg
     1261 cccgacgttc gcatgatgtt gcgcatgtag cgcgggagat cctccgggcg cggctcgaag
     1321 acgacgacgt ccgccgcggg ggcctcgcgg cggtaccgcc cgaggccgta cttgagccgc
     1381 gagtggagcg tgaccctgaa gacctggtcc aggatcgtcg ggaggccgcg ccggcgcaga
     1441 gcgccggcgc cggcgccgtt cgtgtcggcc ggacggaaac ggacggggac gagcgggttc
     1501 acgcagatcg tgaggccgca gcgctcgcgg atcgagagcg agatgtgggc cgtcttcttc
     1561 acgccgccgt cgatgtagtc gacgcccttg atcttcaccg gtcggtagag gccgggaatg
     1621 gcgcaggagg cggcgacggc ctgcgagatc gggacgtcgt cgtggccttc gcagccgaac
     1681 gcggccgtgt tccccgagtc gaggtcgacc gcgacgatcc ggagcgtctt ggccagcttg
     1741 cggaagtcgt tcgtgcgccc gtccctcgtg agccagtcgc gcatccacgc ctcgagcccc
     1801 tcgttgtgga agatgcccga gggcaggagc tggccgaagg cctggacgag gtccgtgagt
     1861 gtcgtctcgt ggcggttctt gtagtagtcc caggcggcgt cgaggagcgt gaacggtgcc
     1921 ttcgcgaggc ggctcgcgat ctcctcgaag ttgacccgga agaggttgag atcgtcgacg
     1981 tcggtacgcg cctcggccga acgcgtggcg ttgcggaaga ggagcgacgg ggtcacgccg
     2041 ttcgcgagga gcgcggagat gtaagcgccc gccgagacgc cgacgaagac gtcgaagtcc
     2101 gtgatcgagg cgttctcgag ccggtcctcg agggccgcga gggctccgat ctcgtagatg
     2161 gcgcccgtga cgccgccgcc cgcgcagacg agacccagct tcttgccgat cggagccggg
     2221 aagggacgga agcgctcacc cgagccttgg tcggggggca tcccgggtct ctcgcggtga
     2281 accgtcatgc tctcttgaaa gtgaaaaggg gcgccttcga tgggcgcccc gagtgtatcc
     2341 gatcctggcc cgggcgctca gcgggcgcgg cggcctttcg cagccggctt gcgcgacttg
     2401 agcgtatcga tcttcttgcc cagcgtgtcg accttggcgg acagcgagcg gagctcgtgg
     2461 cgcgtgggga cgttcaggcg gtggaggccc atctcgacgg ccgagccgac cttgtcgccg
     2521 accatcttcg tgacgtcttc ggcgcggcgg gcggcctgct cgccggcctt ctggacgcgg
     2581 gtcttcacgt ccttcgtgag cttctcgccg aggtccttgc cggactcgac gacgtccatg
     2641 gcgaaggcgc gggggtcctt ccggatggtc tcgctcttct tcgcgaggtc acgggcggcg
     2701 acgcggacct tcttctcggc cttgacggcg gcggtcttcg cctttgcgcc gaggcggtcg
     2761 agctccgtgc cggcgttctt gagcgccttc tggacggggg cggggacctc gaaggggagt
     2821 ttcgaggcgg gcttgcgggt ggtggttctc atcgggctct ccttttccgg gctgctggag
     2881 cggaggcgcg gccgggtttg gcggctgcgc gctcgcgcgg gtttgaaggg aacgacttct
     2941 gggcgagcga ttcgagctgg gcgaggcgtg tcttgatcgc cgcgacttcg cgggcgagct
     3001 cgggctggcc tccgaggcgg ccaccgaggt cgcggacgct gcccgtgaga tccgccaggc
     3061 tctcgcggaa cacgaggccg acgtcgtcgc gcaggcgcgc gccttcctcc caggtcacgc
     3121 ggcctttctc gaccaggccg ccgacgattt tctcgacgtc ctggcgggcg tcctcgaacc
     3181 tgcgggtgag gggagcgagc gtctggccga gcttctccgg ccgggcggcg ttccggaggc
     3241 tttcggcgac ggaagtggcc ccgacgcgct cggcggcccc ggcgacggtc tcggccgctt
     3301 cgctgccggc ccggaggatt cgcgcgagcg tgtccgcgga agcggcgacc gggccctttc
     3361 ccgacgcgag ggcgcgcgag aggacgcggg ccgtgatgtc ctcgccggtg tcggcggcga
     3421 tgacctcgat gcgggtcccg gtcgagaccg cccgggcgag gtcctcgagg gtgacgaaac
     3481 gccgctccct ggcctcgtag agcttgcggt tccgatagcg gacgatgcgt ctcacgtcgc
     3541 tcatgggccc aagataacgc gtcgcgtaat tcctgtcaag gggaatcttg tgcaatgcgt
     3601 catccggggc gccggggcta acattccggc gatgtcggct cccgcgtccg ccccgcctta
     3661 tgcgcccccg gcgttgccga ggggaaccgt cctggcctgc cggtcgtggc tcacggaagc
     3721 cccttaccgc atgctgctca acaacctgga cccggaggtt gccgagcgcc gcgaggatct
     3781 cgtcgtctac ggaggctcgg gaaaggccgc ccggaacccc gaatgcctcg aggcgatcct
     3841 ccgggagctc aggcgactcg gcgacgacga gacccttctc gtccagtcgg gtaaaccggt
     3901 aggcgtgttc cggacgcacc cggatgctcc gagggttctc atcgcgaact ccaacctggt
     3961 gccgaaatgg ggcacgtggg acgaatttcg ccgcctggag gccctcgggc tgacgatgta
     4021 cggacaaatg acggcgggtt cgtggatcta catcggaacg caggggatcc tccaggggac
     4081 gtacgagaca ttcgcggagg ccgcgcggcg cgatctcgga tcgcccgacg gatcgctcgc
     4141 agggcgcctc gccgtcacgg cgggtctcgg cggcatgggc ggcgcccagc cgctggccgt
     4201 cacgatgtgc gggggaacgg cgctggtggc cgaagtcgac gcggcgcgca tcgacaagcg
     4261 cctcgcgacg cgttatctcg acctcaggat cgacgggctg gacgcggcga tcgacggggc
     4321 gctcgcggcc cgcgacgcga aggaagccgt ttcgatcgga gtccgtgcaa acgcggtgga
     4381 cctcctcgag aggcttctga agagaggcat cgtccccgac gtcctcacgg atcagacctc
     4441 cgcccacgac gaactcaatg gctacgttcc gcatgcactc gaaggaattt ccctctcctt
     4501 cgacgatgcg ctgcgcctgc ggcgggagga tgccgatcga tatcgcaggg cggcggtttc
     4561 ctcgatgggc gcgcacgtgg cggcgatgct cgagctcaag cggcgcggcg ccgtgacgtt
     4621 cgactacggc aacaacctgc gcgccggagc ggcccaggcc ggcgtcgcga acgcgtacga
     4681 catcgccggg ttcgtcccgc tctacatccg gcctctcttc tgcgagggca aggggccgtt
     4741 ccggtgggcc gcgctgtcgg gcgatcccgc cgacatcgac gagacggacc gcgccgtcct
     4801 cgagctcttc ccggagaatg ccgcgcttcg ccggtggatc acgctcgcga gcgagcgcgt
     4861 cgcgttccag ggtctgccgg cgcgcatctg ctggctcggc tacggcgagc gcgcgaaggc
     4921 gggccgcgcc ttcaacgagc tcgtgcggag cgggcgcgtg aaggcgccga tcgtgatcgg
     4981 ccgcgaccat ctcgactgcg gttcggtcgc ctcgccgaac cgggagacgg agggcatgaa
     5041 ggacgggagc gacgcgatcg cggactggcc gctcctgaac gcgatgctca acaccgccgc
     5101 cggcgccacg tgggtctcga tccaccacgg cggcggcgtc gggatcggct attcgctgca
     5161 cgcgggaatg gtcgtcgtgg cggacgggac ggacgccgcc gccgtgcggc tcgagcgcgt
     5221 gctcacgacg gatccgggca tgggcgtgat ccggcacgcg gacgcgggct accccgaggc
     5281 cgtggcgtgc gcggaggcga acggcattcc ggttccgatg cgccgttgag cggccggacg
     5341 cgggcccgcc tcgcgctcgg ggcgggaacg gccgcgg
//
LOCUS       DAOMFY010000274         5285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5436_length_5285_cov_6.681453, whole genome shotgun sequence.
ACCESSION   DAOMFY010000274 DAOMFY010000000
VERSION     DAOMFY010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5436_length_5285_cov_6.681453"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLB01_16570"
     CDS             <1..368
                     /locus_tag="PLB01_16570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ78968.1"
                     /translation="IPVFADPVALQRAFRNVLDNAVKYTPSGGQVRVRASVVSQHAFV
                     VISDTGSGMSPEEQKLAFTPSWRSPAAVAAGIPGRGIGLGVTKELLDQNGGKISLLSE
                     QGHGLEVTIMFPLSREGRA"
     gene            365..754
                     /locus_tag="PLB01_16575"
     CDS             365..754
                     /locus_tag="PLB01_16575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ78969.1"
                     /translation="MTPRVFVLDDDPAMVEAVMAVLVHDGFEVDGTSDAAAFLRTVLR
                     TPPDLVVLDVNMPGLTGWEICDILRRQPVTAALPVLFLTGRGEVADRITAMQVGGTDH
                     LTKPFRAEDLRARARVLTGRAAAKGVR"
     gene            751..2097
                     /locus_tag="PLB01_16580"
     CDS             751..2097
                     /locus_tag="PLB01_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQQ78970.1"
                     /translation="MKPTHPRGPLRIVSRQAPPEAAAHPEIEKLRLESLLHDPVTGLT
                     LHPFADLKGERVANLGVVYVQLGRFAGVESLYGWELYDRILRVTAASLREDLASSPFA
                     PGFLSMSFNGSDGVFVLFDLARRTPSRKGGTLETEAARLRVGVVRRMKQALGRSSVDL
                     MHVHATALAVPDDPRVRPARNLMRALAEASRLADARESGEKKSFVERLKAILSGKKLR
                     AVFQPIVAVKTGEVLGYEALIRGPQDHELEMPDDLFAVAREGDLLLELESLCVETVFS
                     SLPRVARGKRLFVNASSRLLTHSVFLDDRNLAEMTRAHGDVVLELSEKEVVFNYPAFR
                     EVVARLRAAGFGFAIDDAGSGYSGLESIVQLRPEYVKVAHTLVSGLHVDRIKREIVGS
                     LAALARRIDAELIAEGIEDPAELASLEALGVEWGQGYLFGRPAARPVVLRPGGPKA"
     gene            2156..2938
                     /locus_tag="PLB01_16585"
     CDS             2156..2938
                     /locus_tag="PLB01_16585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQQ78971.1"
                     /translation="MTSLARNLLFGAGVPLLVGIVWMADRKGAFRRDAFPTPWRKRAA
                     LGLLFLLLVATTLLPAAAGGRPPDVSTLKITQVFLVQGVLAVFLASWWLLAGRPPLRA
                     FLALESKQPGPEAAAGVALGLIGWTLTIVVAVVAAVFLSLVDVQQQRAIPPLVGWLAS
                     RPAVERLLIVISAMTLEEFHFRAFLQRRLGAIPASILFLLAHAGYGEPFFFVGLLAIT
                     SVLAIAFARTGSVVAPMLAHGTFDAVQLFIFLPAALKFLQGS"
     gene            complement(2895..4223)
                     /locus_tag="PLB01_16590"
     CDS             complement(2895..4223)
                     /locus_tag="PLB01_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78972.1"
                     /translation="MIRDAVSTGKGRAPHRFPAFLAAAAALAGALTAAPATPLAQRLP
                     EAVAEAQKLVEKVRGVPFRGAVASALLPEKDLAKILGKKIVEDLPVPFPRYAASLAAV
                     GFLDPEAGLEEKLTKLYARQVAGFYDPEVKKFFIVPERTAEAAAVSPALGLSTGTLLE
                     DALLAHELTHALQDRRLDLVPRMKALKESSDALLALEAFLEGEATVVMMDALLAKLPP
                     ETKDLFGADTLSNMLSGLAAGTTSVDGSEGVPDFFVKEMLFPYVSGTAWIEAKRAGAG
                     WTPVDAAYKSPPTTTAEILHPERTAARALLAPADRPAPADLPQGMKVLYGDSFGEWML
                     ATLLERAGAADAKALAAEWQDDRILFFEPKTSPGEVRPVGFVWRIRVSSPEAAHRLAA
                     ALAPLYERPDGRPVASVTAAGDRVEVVRGRPQAPKRVSSPAGTSGPPAGR"
     gene            4296..4751
                     /locus_tag="PLB01_16595"
     CDS             4296..4751
                     /locus_tag="PLB01_16595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ78973.1"
                     /translation="MSNPPPGPIVLWLSSRYENLELAQAVLEHVCKARGVGTDAEHWM
                     GVALREALANAIKHGNRQDPAKRVYLSLAGGAETIEIVVGDEGQGFEATAVADPLAPE
                     NQMKTSGRGIFYMKTFMDEVTFSRGENGGTILSMKKRLDSETKKGVHTP"
     gene            4748..5089
                     /locus_tag="PLB01_16600"
     CDS             4748..5089
                     /locus_tag="PLB01_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQQ78974.1"
                     /translation="MKTSVTTRDVGDVKIIELHGKITIGAGDLQMREAIHAAVNGGAT
                     KILVDMKDVTTIDSSGVGELVGCYTTATHKGAKLKLQNLPPKISDVLTVTQLITVFDV
                     FPNEKDALASF"
BASE COUNT          729 a         1888 c         1889 g          779 t
ORIGIN      
        1 agatcccggt cttcgccgat ccggtggccc tccagcgcgc gttccgcaac gtcctcgaca
       61 acgcggtcaa gtacacgccg tcgggcgggc aggtgcgcgt gagggcgagc gtcgtctcac
      121 agcacgcctt cgtcgtgatc agcgacacgg ggtccggaat gtcgcccgag gagcagaagc
      181 tcgcgttcac cccgtcctgg cgcagccccg cggccgtcgc ggccggcatc ccggggcgcg
      241 ggatcgggct cggggtcacg aaggagctcc tcgaccagaa cggcgggaag atctcgctcc
      301 tctccgagca ggggcacggg ctcgaggtaa cgatcatgtt cccgctgtcg cgcgagggcc
      361 gcgcatgacc ccgcgcgtct tcgtcctcga cgacgacccc gcgatggtcg aggccgtgat
      421 ggccgtcctc gtccacgacg gtttcgaggt ggacggcacg agcgacgccg cggccttcct
      481 gcggacggtg ctgcgcacgc cgcccgacct cgtcgtcctc gacgtgaaca tgcccggcct
      541 cacgggttgg gagatctgcg acatcctgcg gcgccagccg gtcacggcgg cgctgcccgt
      601 cctgttcctg acggggcgcg gcgaggtcgc cgaccgcatc acggcgatgc aggtcggcgg
      661 cacggaccac ctcacgaaac cgttccgggc ggaggacctg agggcgcgcg cgcgcgtcct
      721 gacggggcgc gccgccgcga agggagtgcg atgaagccga cccacccgcg cgggccgctg
      781 agaatcgtgt cgcgccaggc gccgcccgag gcggccgccc accccgagat cgagaaactg
      841 cggctcgagt cgcttctcca cgaccccgtc acggggctca cgctgcaccc cttcgcggac
      901 ctcaagggag agcgtgtcgc gaacctcggc gtcgtctacg tgcagctcgg gcgcttcgcc
      961 ggcgtcgaga gcctctacgg ctgggagctc tacgaccgca tcctgcgcgt cacggccgcg
     1021 agcctgcgcg aggacctcgc gtcctcgccg ttcgcgccgg gcttcctttc gatgtcgttc
     1081 aacggctccg acggcgtctt cgtgctcttc gacctcgcgc gccggacgcc gagccgcaag
     1141 ggcgggacgc tcgagaccga ggccgcgcgc cttcgcgtcg gcgtcgtgcg gcgcatgaag
     1201 caggcgctcg ggcgctcgtc ggtcgacctc atgcacgtgc acgcaaccgc gctcgcggtg
     1261 cccgacgacc cgcgcgtcag gccggcgcgc aacctcatgc gcgcgctcgc ggaggcctcg
     1321 cgcctcgcgg acgcccgcga gagcggcgag aagaagtcgt tcgtcgagcg cctcaaggcg
     1381 atcctctccg gcaagaagct gcgcgccgtc ttccagccga tcgtggccgt gaagacgggc
     1441 gaggtcctcg ggtacgaggc gctcatccgc ggtccgcagg accacgagct cgagatgccg
     1501 gacgacctct tcgcggtggc ccgggagggc gacctcctcc tcgagctcga gagcctctgc
     1561 gtcgagaccg tgttctcctc gctcccgcgc gtcgcgcgcg gcaagcgcct cttcgtgaac
     1621 gcgtcctcgc gcctcctgac gcactcggtg tttctcgacg accgcaacct cgcggagatg
     1681 acgcgcgcgc acggcgacgt cgtcctcgag ctgtccgaga aggaggtcgt cttcaactac
     1741 ccggcgttcc gcgaggtcgt ggcgcggctc agggccgccg gcttcgggtt tgcgatcgac
     1801 gacgcggggt cgggctactc gggcctcgag tcgatcgtgc agctgcgccc ggagtacgtg
     1861 aaggtcgcgc acacgctcgt ctcgggcctc cacgtcgacc ggatcaagcg cgagatcgtg
     1921 ggctcgctcg ccgcgctcgc ccggcgcatc gacgccgagc tgatcgccga gggcatcgag
     1981 gaccccgccg agctcgcgag cctcgaggcg ctcggcgtcg agtgggggca gggatacctg
     2041 ttcggccggc ccgccgcgcg gcccgtcgtc ctcaggccgg gcgggccgaa agcctgacgg
     2101 acggaacccg cgcgggcggg gctttgctag agtcccgccc ggccgggcca cgcacgtgac
     2161 ctctctcgca cgcaacctcc ttttcggggc gggagttccg ctcctcgtcg gaatcgtgtg
     2221 gatggccgac cggaagggtg cgttccggcg cgacgcgttc ccgacgccct ggaggaagcg
     2281 cgccgcgctc ggactgctgt tcctcctcct cgtcgcgacg acgctcctgc ccgccgccgc
     2341 gggcggccgc ccgccggacg tctcgaccct gaagatcacg caggtcttcc tcgtgcaggg
     2401 ggtcctcgcg gtcttcctcg cttcgtggtg gctcctcgcg ggccgtccgc ccctcagggc
     2461 gttcctcgcg ctcgagtcga agcagcccgg cccggaggcc gcggccggcg tcgccctcgg
     2521 cctcatcggc tggacgctga cgatcgtggt ggccgtcgtc gcggccgtct tcctgagcct
     2581 ggtcgacgtc cagcagcagc gcgccattcc gccgctcgtc ggctggctcg cgagccgccc
     2641 ggccgtcgag cggctgctga tcgtgatctc cgccatgacg ctggaggagt tccacttccg
     2701 cgcgttcctc cagcgccggc tcggggcgat cccggcctcg atcctgttcc tcctcgccca
     2761 cgcgggctac ggggagccgt tcttcttcgt ggggctgctc gcgatcacgt ccgtgctcgc
     2821 gatcgcgttc gcccggacgg gcagcgtcgt cgccccgatg ctcgcgcacg ggacgttcga
     2881 cgccgtgcag ctcttcatct tcctgccggc ggccctgaag ttcctgcagg ggagctgacg
     2941 cgcttcggcg cctgcggccg cccgcgcacg acctcgacgc ggtcgccggc cgcggtgacg
     3001 gacgcgacgg ggcggccgtc gggccgctcg tagagaggcg cgagcgccgc cgcgaggcgg
     3061 tgcgcggcct cgggagagga gacgcggatc cgccagacga acccgacggg ccggacctcg
     3121 cccggcgacg tcttcggctc gaagaagagg atgcggtcgt cctgccactc cgcggcgagg
     3181 gccttcgcgt ccgccgcccc ggcacgctcg agcagcgtcg cgagcatcca ctcgccgaag
     3241 ctgtccccgt acaggacctt catcccctgt gggaggtcgg cgggcgcggg gcggtctgcc
     3301 ggcgcgagga gggcccgcgc ggcggtgcgc tcggggtgga ggatctcggc ggtcgtcgtc
     3361 ggcggcgact tgtaggccgc gtcgacaggc gtccagccgg ctcccgcgcg cttcgcctcg
     3421 atccaggcgg tgccggacac gtaagggaag agcatctcct tcacgaagaa gtccggcacg
     3481 ccctcggagc cgtcgacgct cgtcgtgccg gccgcgaggc ccgagagcat gttcgagagc
     3541 gtgtccgctc cgaacaggtc cttcgtctcg ggcgggagct tcgcgaggag cgcgtccatc
     3601 atgacgacgg tggcctcgcc ttcgaggaac gcctccagcg cgaggagggc gtcggaagac
     3661 tccttgagcg ccttcatgcg cggaacgagg tcgaggcggc ggtcctggag cgcgtgggtg
     3721 agctcgtgcg cgaggagcgc gtcctcgagc agcgtccccg tcgagagccc gagggccggc
     3781 gagacggcgg ccgcctcggc cgtgcgctcg gggacgatga agaacttctt gacctccggg
     3841 tcgtagaaac ccgccacctg ccgcgcgtag agcttcgtga gcttctcctc gaggccggcc
     3901 tccgggtcga ggaagccgac ggcggcgagc gaggccgcat agcgcgggaa cggaaccggg
     3961 aggtcctcga cgatcttctt cccgaggatc ttcgcgaggt ccttttcggg gaggagggcg
     4021 gaggcgacgg ctccccggaa cgggacgccc cggaccttct cgaccagttt ctgggcctcg
     4081 gcgacggctt cgggcagccg ctgtgcgagc ggggtcgccg gggccgccgt cagggcgccg
     4141 gcgagggcgg cggcggccgc caggaacgcc gggaaccggt gcggggccct ccctttcccg
     4201 gtcgagacgg cgtcgcgtat catgcgcccg gtttcagcga acgggagagg tgacacatcg
     4261 gggccagctt aacagcgcga cccttgcaac ttgcgatgag caatccgccg ccgggcccca
     4321 tcgtcctctg gctttcgagc cgctacgaga acctcgagct ggcgcaagcc gtgctcgagc
     4381 acgtgtgcaa ggcgcgcgga gtcgggacgg acgccgagca ctggatgggg gtcgcgctcc
     4441 gggaggcgct cgcgaacgcg atcaagcacg gcaaccggca ggatccggcc aagcgcgtct
     4501 atctgtcgct cgccggcggc gccgagacga tcgagatcgt cgtcggcgac gaggggcagg
     4561 gtttcgaggc gacggcggtg gccgaccctc tcgcgccgga gaaccagatg aagacctccg
     4621 gccgcggtat cttctacatg aagacgttca tggacgaggt gacgttctcg cgaggcgaga
     4681 acggcgggac gatcctctcc atgaagaaga gactggattc cgaaacgaag aagggagtgc
     4741 atacaccatg aagacttcgg tgaccacccg ggacgtcggc gacgtcaaga tcatcgagct
     4801 gcacggaaag atcacgatcg gcgcgggcga cctccagatg cgcgaggcga tccacgcggc
     4861 ggtcaacggc ggggcgacga agatcctcgt cgacatgaag gacgtcacga cgatcgactc
     4921 gtcgggcgtc ggcgagctcg tgggctgcta caccacggcg acgcacaagg gcgcgaagct
     4981 gaagctccag aacctgcccc cgaagatcag cgacgtcctc acggtgaccc agctcatcac
     5041 cgtgttcgac gtgttcccga acgagaagga cgcgctggcg agcttctgaa gcaatcgggg
     5101 ggtgcgtcga ccgttcgtgc tcggagtgcg ccgtgaccgc accccccgtg cccccctctg
     5161 ccctcgcgga cctgctggcc caggtgcgcg tcgcggtcga cgcgcgcctc cccggcctcc
     5221 tcgcgccgcc gctcggggag cctgacccgc tcgaccccct cccgcccgcg gtgcgcgagg
     5281 ccctg
//
LOCUS       DAOMFY010000275         5268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5472_length_5268_cov_8.098983, whole genome shotgun sequence.
ACCESSION   DAOMFY010000275 DAOMFY010000000
VERSION     DAOMFY010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_5268_cov_8.098983"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..682
                     /locus_tag="PLB01_16605"
     CDS             104..682
                     /locus_tag="PLB01_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQQ78975.1"
                     /translation="MPSPLKAPVPAPVDAMGEPLVVALAAAVPGSVLGAKKMVGQTTI
                     EVAPDKLVEACRHLKDKETYTFLVDQTAVDWKERAPRFDVVYWLHSFDRGNARVRLKV
                     GVADGAACPTVSGVWETANWMEREVFDLFGISFEGHPDLRRILTWDGFQGHPLRKDFP
                     VEGIDTGAAIYPDRYPEGGGPAPDDKNRKTVS"
     gene            730..1836
                     /locus_tag="PLB01_16610"
     CDS             730..1836
                     /locus_tag="PLB01_16610"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQQ78976.1"
                     /translation="MLGLREMELNFGPQHPSTHGVLRIVLKVDGERIVSARPDVGYLH
                     RGTEKLFETETYPMVIPHTDRMDYVAAATNNHAYVLAVEKLLGIDVPKRAQYIRVVLD
                     EMQRISSHLLWLATAAIDLGAITPFFYTFREREVILDLFEDYCGARLTLNCMRVGGLQ
                     EELTPGWIEKLRAFTEDLGTKIDEYEDLLTENRIWKMRTIGIGILNTERCIEWGVTGP
                     PLRAAGLAWDIRRALPYECYPELDFEIPTGKNADTYDRYLVRIAELRQSRRILLQCCD
                     WLAANPTGDIRGKVPRVIKPPAGDVYASVESPKGELGFYLVSNGGNKPYRLRVRPPSF
                     INLQALPEMARGHMVSDLVAIIGTIDIVLGEVDR"
     gene            1838..3040
                     /gene="nuoH"
                     /locus_tag="PLB01_16615"
     CDS             1838..3040
                     /gene="nuoH"
                     /locus_tag="PLB01_16615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQQ78977.1"
                     /translation="MEISETVLNVVLIPAVKAVVVVILVATAAGVLTYIERRTLAFLQ
                     VRKGPNRVGPEGILQFVADVVKLLTKEDVVPAKAQHFVHFLAPILVMVPGLAVFAMLP
                     WGPEFTVRIPGLGKITTAWYATDLNVSLLLLLAITSVGIYGIILGGWASNSKYALLGG
                     LRSAAQLISYEVPMGFAVVSAILMARSLSLVEIVEAQRTHHMWFILPGLVSFFLYFVS
                     GVAETNRIPFDLPEAESELVAGFHTEYSGFRWAIFFLTEYANMITISAVATALFFGGW
                     LRPFPNVAALGFLDVIPGVIWYVLKVAGFLFVYIWFRGTFPRYRFDQLMALGWKTLIP
                     ISLVNLVLVALAALGGWRGLRILGAILWVAAIGAVLIVTRLRAAKKPPATRLAPAPVG
                     AGPIKAEA"
     gene            3042..3518
                     /locus_tag="PLB01_16620"
     CDS             3042..3518
                     /locus_tag="PLB01_16620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HQQ78978.1"
                     /translation="MSFWDDVLLLDIFGGLKMTGKHFFRKADTVEYPEAAREPSDRFR
                     GMFRLDEERCIKCTLCAIECPINIIFIDWHNEKNPQGKNEKVLDRFDVDLKRCMFCGL
                     CEEACPTNPKSIWLRTKTYELGTYERNEALYLDIKKLTKWDENVKPFTVVEQGEGR"
     gene            3515..4120
                     /locus_tag="PLB01_16625"
     CDS             3515..4120
                     /locus_tag="PLB01_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQQ78979.1"
                     /translation="MTAPFFFWFFAAIVVFGAVGVVAMRQPVHSALFLLLSFFGIAAL
                     FVTLNAEFLAAVQILVYAGGIMVLFLFTIMLVNIRRTAGEPFLHRQAGGALLVAFLLL
                     AVVGGTIVGSSRTSSLADAGQPADPAKLATTAITRTVTETVDGKVVKTPVTEEVAGNS
                     QAVAWSLYRDYLLPFEVASVFLLVAMIGAVVLGKRTMERVD"
     gene            4120..4422
                     /gene="nuoK"
                     /locus_tag="PLB01_16630"
     CDS             4120..4422
                     /gene="nuoK"
                     /locus_tag="PLB01_16630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQQ78980.1"
                     /translation="MISVTHYVALSFLLFAIGVTGVLIRRNLLTVLMSIELILNAANL
                     NLIAFSRQLGDLNGQLFTVFVITLAAGEAAIGLAIIISLYRLKNSVNLDAVSELKG"
     gene            4426..>5268
                     /locus_tag="PLB01_16635"
     CDS             4426..>5268
                     /locus_tag="PLB01_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQQ78981.1"
                     /translation="MKAVAILPWIPVFPLIGFLVNGLLYLVSHAKLGGKDAPKGPHGH
                     GDGGHADTHAGHGDAGHGGGHGEHHEIPFAAVHAVVGPAATILSCVAAFAAIFLWWGE
                     THGHEGVVATLWTWMPLGANPTWFGGKALVVDAAFRLDALSALMLGFVTFIGSLIHVY
                     SVGYMGHDEGFGRYFSYLNLFMFSMLTLVLGAGLPILFVGWEGVGLCSYLLIGFYWKK
                     DWAADAGKKAFVVNRIGDFGFVLGIFGCFALFGTADFGAMFSMAAADPAKYAAGGLLT
                     AVCLA"
BASE COUNT          795 a         1851 c         1708 g          914 t
ORIGIN      
        1 caacgagcgc acccggaagc tgccgcgatc ggcgaagacg acgctgccag cagggcccgc
       61 cgctaggcct tggggcaggc tcaggtaagc cgcggtcgct gctatgccgt cgccattgaa
      121 ggcgcccgtg cccgcgcccg tcgacgcgat gggggagccg ctcgtcgtcg cccttgccgc
      181 ggccgtgccc ggctcggtcc tcggcgcgaa gaagatggtc ggccagacga cgatcgaggt
      241 cgcgcccgac aagctcgtcg aagcctgccg gcacctcaag gacaaggaaa cgtacacgtt
      301 cctcgtggac cagacggccg tggactggaa ggagcgcgcc ccgcgcttcg acgtcgtcta
      361 ctggctgcat tcgttcgacc gcggcaacgc gcgcgtgcgg ctcaaggtcg gcgtcgccga
      421 cggcgcggcc tgcccgaccg tgagtggcgt ctgggagacg gcgaattgga tggagcgcga
      481 ggtgttcgac ctcttcggca tctcgttcga gggccacccc gacctccgac ggatcctcac
      541 gtgggacggc ttccagggcc atccgctccg caaggacttc cccgtcgagg gcatcgacac
      601 gggcgccgcg atctacccgg accgctaccc cgagggcggc ggccctgcgc ccgacgacaa
      661 gaacaggaag acggtctcgt gagcgagctc gtcggaaccg ccggcgccgt ccccgaatcg
      721 gacgagacga tgctcgggct ccgcgagatg gagctcaact tcgggccgca gcacccgtcc
      781 acgcacggcg tcctccggat cgtcctgaag gtggacggcg agcggatcgt ctcggcgcgc
      841 ccggacgtcg gctacctcca ccgcggcacc gagaagctct tcgagaccga gacgtacccc
      901 atggtcatcc cgcacacgga ccgtatggac tacgtcgcag cggcgacgaa caaccacgcg
      961 tacgtgctgg ccgtggagaa gctcctgggc atcgacgtcc cgaagagggc gcagtacatc
     1021 cgcgtcgtcc tcgacgagat gcagcggatc tcgtcgcacc tcctctggct cgcgacggcg
     1081 gcgatcgacc tcggcgcgat cacgccgttc ttctacacgt tccgcgagcg cgaggtcatc
     1141 ctcgacctct tcgaggacta ctgcggcgcg cgcctcacgc tgaactgcat gcgcgtcggc
     1201 ggtctccagg aggagctgac gcccggctgg atcgagaagc tccgtgcgtt cacggaagat
     1261 ctcggcacga agatcgacga gtacgaggac ctcctgaccg agaaccgcat ctggaagatg
     1321 cggacgatcg gcatcggcat cctgaacacc gagcgctgca tcgagtgggg cgtcacgggc
     1381 ccgccgctgc gcgccgccgg gctcgcgtgg gacatccgcc gcgcgctccc ctacgagtgc
     1441 taccccgagc tcgacttcga gatcccgacc gggaagaacg ccgacacgta cgaccgctac
     1501 ctcgtgagga tcgccgagct ccgccagagc cgccggattc tcctccagtg ctgcgactgg
     1561 ctcgccgcga accccacggg cgacatccgc ggcaaggtgc ctcgcgtcat caagccgccg
     1621 gcgggcgacg tgtacgcgag cgtggagtcc ccgaagggcg aactcggctt ctacctcgtc
     1681 tccaacggcg ggaacaagcc gtaccggctg cgcgtgcgtc cgccgagctt catcaacctt
     1741 caggcgctgc cggagatggc gcgcggccac atggtctcgg acctcgtcgc gatcatcggc
     1801 acgatcgaca tcgttctggg ggaggtcgat cgctgacatg gaaatctccg aaaccgtcct
     1861 caacgtcgtc ctgatcccgg ccgtcaaggc cgtcgtggtc gtgatcctcg tcgccacggc
     1921 ggcgggggtc ctcacgtaca tcgagcgccg gaccctcgcg ttcctccagg tgaggaaagg
     1981 ccccaaccgg gtcggccccg agggaatcct ccagttcgtc gccgacgtcg tgaagctcct
     2041 cacgaaggaa gacgtcgttc cggccaaggc ccagcatttc gtccacttcc tcgcgccgat
     2101 cctcgtgatg gtcccgggcc tcgcggtctt tgcgatgctg ccctgggggc ccgagttcac
     2161 ggtgaggatt ccgggcctcg ggaagatcac gaccgcctgg tacgcgacgg acctcaacgt
     2221 ctccctgctg ctcctgctcg cgatcacgtc ggtggggatc tacgggatca tcctcggggg
     2281 ctgggcgtcg aactcgaagt acgccctcct cggcgggctc cgctcggcgg cgcagctcat
     2341 cagctacgag gtcccgatgg gcttcgcggt cgtgagcgcg attctcatgg cgcgctcgct
     2401 gtcactcgtc gagatcgtcg aggcgcagcg gacgcaccac atgtggttca tccttcccgg
     2461 cctcgtctcg ttcttcctgt acttcgtgtc gggcgtcgcg gagacgaacc gcatcccgtt
     2521 cgacctgccc gaagccgaga gcgagctcgt cgcgggcttc cacacggagt actccggctt
     2581 ccggtgggcc atcttcttcc tgaccgagta cgcgaacatg atcacgatct cggcggtcgc
     2641 gacggcgctg ttcttcggcg gctggctgag gccgttcccg aacgtcgccg ccctcgggtt
     2701 cctcgacgtg atcccgggcg tgatctggta cgtcctgaag gtcgcgggtt tcctcttcgt
     2761 ctacatctgg ttccgcggga cgttcccgcg ctaccgcttc gaccagctca tggcgctcgg
     2821 gtggaagacc ctgatcccga tctccctcgt gaacctcgtc ctcgtcgcgc tcgcggccct
     2881 gggcgggtgg cgcgggctcc ggatcctcgg cgcgatcctc tgggtcgcgg cgatcggcgc
     2941 cgtcctcatc gtgacgcggc tccgggcggc caagaagccg cctgcgactc gtttggctcc
     3001 ggcgccggtc ggcgccggcc ccatcaaggc ggaggcctga cgtgagtttc tgggacgacg
     3061 tcctcctcct cgacatcttc ggcggcctca agatgaccgg gaagcacttc ttccggaagg
     3121 ccgacaccgt ggagtacccc gaggccgccc gcgagccgag cgaccgattc cgcgggatgt
     3181 tccggctcga cgaggagcgg tgcatcaagt gcacgctctg cgccatcgag tgcccgatca
     3241 acatcatctt catcgactgg cacaacgaga agaacccgca gggcaagaac gagaaggtcc
     3301 tcgaccggtt cgacgtggac ctcaagcgct gcatgttctg cggcctctgc gaggaagcct
     3361 gcccgacgaa tccgaagtcg atctggctga ggacgaagac gtacgagctc gggacgtacg
     3421 agcggaacga ggccctctac ctcgacatca agaagctcac gaagtgggac gagaacgtca
     3481 agccgttcac ggtcgtcgag cagggggagg gacggtgacc gcgccgttct tcttctggtt
     3541 ctttgcggcg atcgtcgtct tcggggccgt cggcgtcgtc gcgatgcgcc agccggtcca
     3601 ctccgcgctc ttcctcctcc tctccttctt cggaatcgcg gcgctcttcg tgacgctgaa
     3661 cgccgagttc ctcgcggcgg tccagatcct cgtctacgcg ggcgggatca tggtcctctt
     3721 cctcttcacg atcatgctcg tgaacatccg gaggacggcg ggcgagccct tcctgcaccg
     3781 gcaggccggc ggggcgctcc tcgtcgcgtt cctgctcctc gcggtcgtcg gcgggacgat
     3841 cgtcggctcc tcgcggacga gcagcctcgc ggacgcgggg cagcccgcgg accccgcgaa
     3901 gctcgcgacg acggcgatca cgcgcaccgt caccgagacc gtcgacggga aggtcgtgaa
     3961 gacccccgtc accgaggagg tcgccggcaa cagccaggcc gtcgcctgga gcctctaccg
     4021 cgactatctt ctcccgttcg aggtggcctc cgtcttcctc ctcgtcgcga tgatcggggc
     4081 ggtcgtcctc ggcaagcgga cgatggagcg ggtggactga tgatttcggt cacgcactac
     4141 gtcgccctgt cgttcctgct cttcgcgatc ggggtgacgg gcgtcctgat ccgtcgcaac
     4201 ctcctcaccg tcctcatgtc gatcgagctc atcctgaacg ccgcgaacct caacctcatc
     4261 gcgttctccc ggcagctcgg cgacctgaac ggccagctct tcacggtctt cgtgatcacg
     4321 ctcgccgccg gcgaggccgc gatcggcctc gcgatcatca tctccctcta ccgcctcaag
     4381 aactccgtga acctcgacgc cgtgtcggag ctgaagggat aacgcgtgaa ggccgtcgcg
     4441 atcctgccct ggatccccgt cttcccgctg atcgggttcc tcgtcaacgg cctgctctac
     4501 ctcgtctccc acgcgaagct ggggggcaag gacgcgccga agggtccgca cggccacggc
     4561 gacggcggac acgccgacac ccatgccggg cacggggacg ccggacacgg cggcggacac
     4621 ggggagcacc acgagatccc cttcgcggcg gtccacgcgg tcgtgggccc cgcggcgacg
     4681 atcctctcgt gcgtggcggc ctttgccgcg atcttcctgt ggtggggcga gacgcacggc
     4741 cacgagggcg tcgtcgccac gctctggacc tggatgcctc tcggcgcaaa cccgacctgg
     4801 ttcggcggca aagcgctcgt cgtcgacgcg gcgttccggc tcgacgcgct ctcggcgctg
     4861 atgctcgggt tcgtcacgtt cataggctcc ctgatccacg tctactccgt cgggtacatg
     4921 gggcacgacg agggcttcgg gcggtacttc tcgtacctga acctcttcat gttctcgatg
     4981 ctcacgctcg tcctcggcgc gggactcccg attctcttcg tcgggtggga gggcgtgggc
     5041 ctctgctcgt acctcctgat cggcttctac tggaagaagg actgggccgc ggacgccggc
     5101 aagaaggcgt tcgtcgtcaa ccgcatcggc gacttcggct tcgttctcgg gatcttcggc
     5161 tgtttcgcgc tcttcgggac ggccgacttc ggcgcgatgt tctccatggc ggccgccgac
     5221 cccgcgaagt acgccgcggg cggcctcctg acggccgtct gcctcgcg
//
LOCUS       DAOMFY010000276         5236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5527_length_5236_cov_6.997684, whole genome shotgun sequence.
ACCESSION   DAOMFY010000276 DAOMFY010000000
VERSION     DAOMFY010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5527_length_5236_cov_6.997684"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLB01_16640"
     CDS             <1..682
                     /locus_tag="PLB01_16640"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HQQ78982.1"
                     /translation="ALLALLAPAAAGAPPAVVVRVFALKNRAAGEALQLVRPLLSDAG
                     SVMLDPKANELTVRDTAASVERAAQAVAAYDVPLRGVDVAVTLLKATSDPKAAPSMAD
                     ASAEIRGIGERLKKLFNVTDVSRVDSVIVRGVEGQRVAYAIGATYHLQFFLDPSRDGR
                     RLQFRDLSLERVHRESGKETRGEILHTSINVNMGQPYILGVGKDEAARSALFLVFYPE
                     WSRPGPGI"
     gene            682..1929
                     /locus_tag="PLB01_16645"
     CDS             682..1929
                     /locus_tag="PLB01_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQQ78983.1"
                     /translation="MATYVCRLGLPDGAIATRTIEAPDEGALRAEIARLNARLFSVKV
                     AGDEPKASAPGRGVALRRLSFGSRRPVKQSEFLVFNQELVALLKAGLPIVAGFEILLE
                     RQENARFKGILLDVREQLVSGVALSDAFLSHGDVFPRLYATSLKAGERSGEVEKVLRR
                     YLAYQKILAALRRKVTGALVYPAVLIGLSIGLVTILMTYVIPRFREFFAGFEAGRLPL
                     ITRVVIGTADFLRGHILFVLAGLVLAAFAFTRWKETDAGRLGWDSILLKIPLVGSILQ
                     QFALSQFSRSLATLVGAGTPVVPALEIAAGAVANRRISGAVASVVPRVREGAEVWRSL
                     ESTGVFTSLAVEMIKVGEATGALEEMLTNVSDFYDEAIDAQLQRIVSLIEPIILVVMG
                     GVIATLLLSIYLPMFTILSNIKA"
     gene            1932..3566
                     /locus_tag="PLB01_16650"
     CDS             1932..3566
                     /locus_tag="PLB01_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQQ78984.1"
                     /translation="MSTVRNDSVDTAMPRFLDAASEETRARQLAERLGLPYVDLWTFR
                     IDAEVFRSIPLEWMLRFEFVPERREDGTLSVVMADPSDVVKRDELEALLKARLKVRVG
                     SRAALQDILQKSESTQRVLEEATEEFRMQVVREDEEGQEVLSIDRITADASPIIKLVD
                     SVLFNAIQRRASDIHIETQEHVVVIKYRIDGVLYPAMEPIDKKHHQTIISRIKVMSEL
                     DIAEKRIPQDGRFKVRVKGRTIDFRVSIMPTVHGEDSVIRILDKESANAEFKNLRLDV
                     LGMDPETMRRLRKFIREPYGMVLVTGPTGSGKTTTLYACLSEIQTVEDKIITIEDPVE
                     YQLRGITQIPVNEKKGLTFARGLRSILRHDPDKVMVGEIRDEETAQIAVQAALTGHLV
                     FTTVHANNVVDVLGRFLNMKVELYNFVSALNCILAQRLVRKICPHCRQPFVPTPQLLE
                     ESALAAERLKGVTLYEGRGCIECNGTGFFGRMAITELLDLSDQIRSLILDKRPAAEIK
                     KAAQNEGMTFLRESAIKKVLAGETTLREINKVTFVE"
     gene            3559..4518
                     /locus_tag="PLB01_16655"
     CDS             3559..4518
                     /locus_tag="PLB01_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78985.1"
                     /translation="MSDKNPFSRSFWRSPRPPHAFVLGRDRLVYVGPQEPAKKAVLGA
                     APALRAVVRTLPEDAFTPGPGDVPVAGPAVAGALQRLLAEVGVKVQAASLVVPDGFVK
                     LLAVDVEDADRNHRETDEMIAWKFGRAFGEPAPPLRLSWHPAGPGATGTRILALAAPE
                     SAAASWEAPFEKVGIRIGALETGAFAVSALAKPAPAGDSFLVWADGDTATAVFFVDGA
                     LRFARTRPVFGDADEALHEIRLSAAYLSAGNGGDDLAAPCTAGPEGAAVVEALAAFRR
                     TQGFAAPARLTLSTLVPGASVAPAASASDPAILMGLGALAGDR"
     gene            4520..5161
                     /locus_tag="PLB01_16660"
     CDS             4520..5161
                     /locus_tag="PLB01_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78986.1"
                     /translation="MAGRFNLARRPFVDTRPANLTAGILAVLVLGVSFVAVRTVFRYL
                     DDSRRTREAITQLKAEIARLEDERSRAEASLARVDVAALAADVDEANAIALRRAFSWT
                     RFLTRLEKTLPADVRIAVISLQKAAASPGTAGGVRGVSGESMTVGLTLISRDPNGLPK
                     TIRAFDASPWFERPAPASEDRGEKSVPEGRRLTLSVLYRDVEGRPAPAEGGAR"
BASE COUNT          752 a         1912 c         1785 g          787 t
ORIGIN      
        1 cgccctcctc gcgctcctcg ccccggcggc ggcgggcgcg cctcccgccg tggtcgtgcg
       61 cgtcttcgcg ctcaagaacc gcgcggccgg cgaggcgctc cagctcgtgc gcccgcttct
      121 ctcggacgcg ggctccgtga tgctcgatcc gaaggcgaac gagctcacgg tccgcgacac
      181 ggcggcgtcc gtcgaacggg cggcgcaggc agtcgcggcc tacgacgtcc cccttcgggg
      241 agtcgacgtg gccgtcacgc tcctgaaggc gacgtccgac ccgaaggccg cgccgtcgat
      301 ggccgacgcg tccgcggaga tccgtggaat cggcgagcgc ctcaagaagc tcttcaacgt
      361 gacggacgtc tcccgcgtcg actcggtcat cgtgaggggc gtggagggac agcgcgtcgc
      421 ctatgcgatc ggcgccacgt accacctcca gttcttcctc gacccgtcgc gcgacgggag
      481 gcgcctgcag ttccgcgacc tctcgctcga aagggtgcac cgcgagagcg gcaaggagac
      541 gcgcggcgag atcctccaca cgtcgatcaa cgtgaacatg ggccagccgt acattctcgg
      601 cgtgggcaag gacgaggcgg cgcgcagcgc cctcttcctc gtcttctacc ccgagtggtc
      661 ccggcccggc ccggggatct gatggcgacg tacgtctgcc ggctcgggtt gcccgacggg
      721 gcgatcgcga cgcgcacgat cgaggcgccc gacgagggcg cgctgcgcgc cgagatcgcg
      781 cgcctgaacg cgcgcctgtt ttccgtgaag gtcgccgggg acgagccgaa ggcgtccgcc
      841 ccgggccgcg gagtcgcgct tcgccgcctc tcgttcgggt cgcgccgtcc ggtgaagcag
      901 tccgagttcc tcgtcttcaa ccaggagctc gtggcgctcc tcaaggccgg acttccgatc
      961 gtcgccgggt tcgagatcct cctcgagcgc caggagaacg cgcgcttcaa ggggatcctc
     1021 ctggacgtcc gcgagcagct cgtctcggga gtcgcgctct cggacgcgtt cctctcccac
     1081 ggcgacgtct tcccgcgcct ctacgcgacg tcgctcaagg ccggggagcg ctccggcgag
     1141 gtcgagaagg tcctgcggcg ctacctggcc taccagaaga tcctcgcggc gttgcgccgc
     1201 aaggtcacgg gcgcgctcgt ctacccggcg gtgctgatcg gcctttcgat cggactcgtc
     1261 acgatcctca tgacgtacgt gattccccgc ttccgcgagt tcttcgcagg cttcgaggcg
     1321 gggaggcttc cgctcatcac gcgcgtcgtg atcggaacgg cggacttcct ccggggccac
     1381 atcctcttcg tgctcgcggg gctcgtcctg gcggcgttcg ccttcacgcg gtggaaggaa
     1441 accgacgcgg ggcggctcgg gtgggacagc atcctcctga agatcccgct cgtcggctcc
     1501 atcctccagc agttcgcgct ctcgcagttc tcccgttcgc tcgcgacgct cgtcggcgcg
     1561 gggaccccgg tggtccccgc gctcgagatc gcggcgggcg ccgtcgcgaa ccggcggatc
     1621 tcgggcgccg tggcgtcggt cgtgccgcgc gtgcgcgaag gagccgaggt ctggcggagc
     1681 ctggagtcga ccggcgtctt cacgtcgctc gcggtcgaga tgatcaaggt cggagaagcg
     1741 acgggcgcgc tcgaggagat gctcacgaac gtgtccgact tctacgacga ggcgatcgac
     1801 gcgcagctcc agcgcatcgt gagcctcatc gagccgatca tcctggtcgt catgggcggc
     1861 gtcatcgcga cgctgctcct gtcgatctac ctcccgatgt tcacgatcct ctcgaacatc
     1921 aaggcctgac catgagcacg gttcgcaacg actccgtcga cacggcaatg ccgcgcttcc
     1981 tcgacgcggc gtccgaggag acgcgcgccc ggcagctcgc cgagcgtctc ggcctcccgt
     2041 acgtggacct ctggacgttc cgcatcgacg cggaggtctt ccggtcgatc ccgctcgagt
     2101 ggatgctccg gttcgagttc gtgcccgagc gccgggagga cgggacgctg tcggtcgtga
     2161 tggccgaccc ctcggacgtc gtgaagcgcg acgagctcga ggccctcctc aaggcgcgcc
     2221 tgaaggtccg ggtcggttcg cgcgcggcgc tccaggacat cctccagaag tccgagtcga
     2281 cgcagcgcgt cctcgaagag gccacggagg aattccggat gcaggtggtc cgcgaggacg
     2341 aggagggcca ggaggtcctt tcgatcgacc ggatcacggc ggacgcgagc ccgatcatca
     2401 agctcgtcga ctcggtgctg ttcaacgcga tccagcgccg cgcgtcggac atccacatcg
     2461 agacgcagga gcacgtcgtc gtcatcaagt accgcatcga cggcgtcctc tacccggcga
     2521 tggagccgat cgacaagaag caccaccaga caatcatcag ccgcatcaag gtcatgtccg
     2581 agctcgacat cgccgagaag cgcatcccgc aggacggccg tttcaaggtg cgcgtgaagg
     2641 gccggacgat cgacttccgc gtctcgatca tgccgaccgt tcacggcgag gacagcgtca
     2701 tccggatcct cgacaaggag tccgcgaacg cggagttcaa gaacctgcgc ctcgacgtcc
     2761 tcgggatgga ccccgagacc atgagacggc tccgcaagtt catccgggag ccgtacggca
     2821 tggtcctcgt gacggggccg acgggctcgg gcaagacgac cacgctctac gcgtgcctgt
     2881 cggagatcca gacggtcgag gacaagatca tcacgatcga ggatcccgtc gagtaccagc
     2941 tccgcggcat cacgcagatc cccgtgaacg agaagaaggg gctcacgttc gcgcgcggcc
     3001 tgcgctcgat cctccgccac gacccggaca aggtcatggt cggcgagatc cgcgacgagg
     3061 agacggctca gatcgccgtc caggccgccc tcacgggcca cctcgtcttc acgaccgtcc
     3121 acgcgaacaa cgtcgtcgac gtcctcgggc gcttcctgaa catgaaggtc gagctctaca
     3181 acttcgtctc ggccctcaac tgcatcctcg cccagcggct cgtccgcaag atctgcccgc
     3241 actgccgcca gcccttcgtc ccgaccccgc agctcctcga ggaatccgca ctcgcggccg
     3301 agcgcctgaa aggcgtcacg ctctacgagg ggcgcggctg catcgagtgc aacgggacgg
     3361 gcttcttcgg ccggatggcg atcacggaac tcctcgacct ctcggaccag atccgcagcc
     3421 tcatcctcga caagcggccg gccgccgaga tcaagaaagc ggcccagaac gagggcatga
     3481 cgttcctgcg cgagagcgcg atcaagaagg tcctggccgg ggagaccacg ctccgcgaga
     3541 tcaacaaggt gacgttcgtt gagtgacaag aaccccttct cgaggtcgtt ctggcgctcg
     3601 ccgcggccgc cgcacgcctt cgtgctcggg cgggaccggc tcgtctacgt gggcccgcag
     3661 gagccggcca agaaggccgt gctcggcgcc gcgccggcgc tccgcgcggt cgtgcggacc
     3721 ctgccggagg acgccttcac gcccgggccg ggcgacgtcc ccgtggccgg cccggccgtc
     3781 gcgggcgcgc tccagcgcct cctcgcggaa gtcggcgtga aggtccaggc cgcgtccctc
     3841 gtcgtgcccg acgggttcgt gaagctcctc gcggtcgacg tcgaggacgc cgaccgcaac
     3901 caccgcgaga cggacgagat gatcgcgtgg aagttcggcc gcgccttcgg tgaaccggcg
     3961 ccgccgctcc gcctctcgtg gcatcccgcc ggccccggcg ccacgggaac ccgcatcctc
     4021 gccctcgcgg cgcccgagtc ggcggcggcc tcctgggaag cgcccttcga aaaggtcgga
     4081 atccggatcg gcgcgctcga gaccggcgca ttcgccgtct ccgcgctcgc gaagccggcg
     4141 cccgccgggg attcgttcct cgtgtgggcg gacggcgaca cggcgacggc ggtcttcttc
     4201 gtggacggcg cgctgcgctt cgcgcggacg cgcccggtct tcggcgacgc ggacgaggcc
     4261 cttcacgaga tccgcctgtc ggcggcgtac ctctccgcgg gcaacggcgg cgacgatctc
     4321 gcggcgccgt gcacggccgg gcccgagggc gcggccgtcg tcgaggcgct cgcggcgttc
     4381 cggcgaacgc agggcttcgc cgccccggcg cggctcacgc tctccaccct cgtcccgggc
     4441 gcgagcgtcg ctccggccgc gtccgcctcc gatccggcga tcctcatggg cctcggcgcc
     4501 ctcgcggggg accgctgaca tggccggccg cttcaacctc gcgcgccgcc cgttcgtcga
     4561 cacgcgcccc gcgaacctga cggccgggat cctcgcggtg ctcgtcctcg gcgtctcgtt
     4621 cgtggccgtg aggaccgtgt tccgctacct ggacgactcg cgccgcacgc gcgaggccat
     4681 cacgcagctg aaggccgaga tcgcgcgcct cgaggacgag cgctcgcgcg ccgaggcctc
     4741 gctcgcccgc gtcgacgtcg ccgcgctcgc cgccgacgtg gacgaggcga acgcgatcgc
     4801 gctccgccgc gcgttctcgt ggacgcggtt cctgacacgt ctcgagaaga ctcttcccgc
     4861 ggacgtgcgc atcgccgtca tctcgctcca gaaggccgcg gcgtcgcccg gcacggccgg
     4921 aggggtgcgc ggggtgtcgg gcgagtcgat gaccgtcggc ctcaccctga tctcgcgcga
     4981 cccgaacgga ctgccgaaga cgatccgggc cttcgacgcc tcgccgtggt tcgagcggcc
     5041 ggcgcccgcg agcgaggacc ggggcgagaa gagcgtgccg gagggccggc gcctgacgct
     5101 ctccgtcctc taccgggacg tcgagggccg cccggctccg gcggaaggag gcgcccggtg
     5161 agcgcgcccg ccgcctcctc cggttccgcc gcgggggacc gcgccctcgc cgtcctgcgc
     5221 tccgggctcg accgcg
//
LOCUS       DAOMFY010000277         5217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5558_length_5217_cov_6.619915, whole genome shotgun sequence.
ACCESSION   DAOMFY010000277 DAOMFY010000000
VERSION     DAOMFY010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5558_length_5217_cov_6.619915"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PLB01_16665"
     CDS             complement(<1..1386)
                     /locus_tag="PLB01_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ78987.1"
                     /translation="MERELGKGAMGVVYLGRDPVIGRRVALKTIRATAEDDNEQREFN
                     ERFMREAQAAGTLSHPNIVTIHDVGEEVETQTSFIAMEYVEGKNLKQLLKDKVAFSWD
                     RVAEIAMSVADALDYAHRRGIVHRDVKPANIIILTSDGTVKITDFGIAKIETSSLTET
                     GQFLGTPNYMSPEQVTGEEVDGRSDLFSLGVVLYELLTKRKPFIGDNLTSISYKIVHE
                     DFPPPETYDATIPGEFGPILARALAKDPAARFQSGKDFYAALAEFRTRHAEMEMLKDL
                     GEMVAQAEKLGPVSTVESKKTPLAEVPPPPQAGVSKGPAGLGGGATSLEDLARRGRSD
                     LNPALVGPAPTNLESSIPDWSLDTDALKSPAEREAAKKREREIPEPVSSPGTLVSDVQ
                     RGLKKPAVTPPSAPVSDEENRPTERIQTIPGLVASAAPVSQASVPTPPRGVPPLPGPA
                     VPRVAPPPTPVR"
     gene            1641..3731
                     /gene="fusA"
                     /locus_tag="PLB01_16670"
     CDS             1641..3731
                     /gene="fusA"
                     /locus_tag="PLB01_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQQ78988.1"
                     /translation="MKVYPASAIHNVAVVGHNGVGKTTLVSALLHEAGATPRFGRVEE
                     GNAPTDFDPEEVERKISIGLAVASLEWKKKKINLIDTPGYGIFVSEMLCGLRAADAAI
                     IVVDAVAGVEVQTEKAWKVCDELGLPRLVVVNRMDRERADGGRVMDQLQKKFGRACVC
                     VEAPIGREKAFRGVIDLVAMQGWLTDDAGKTAPGTIPDEFAADAAQLHETLVELVAEG
                     DDKLMETFFAQGNLTEEQMKPALKKAIQERKIAPVLFTASGSHETGVEKLLDEIEDLL
                     PTADAFTSVATGKSGSAEALAPDPAGPAAAFVFKTISDPYAGRLTLFRVYSGTIKGDG
                     TYWNASRELAERFGPVFSPLGKTLQTVPEIPAGDIGGVAKLKETLTGDSLSTKDKPFV
                     FDKIAFPEAAISFAIEPKSKGDEEKISVALAKLIEEDPSLHFSRDTDTHEFLLSGAGQ
                     LHVEIAVARMKRKSGVEVILHPPKVPYRETITRAAEAHGRHKKQTGGHGQFADCKIRV
                     KPLSRGADFEFFDDTFGGSIPRNYIPAIEKGIQEIRKRGFLAGFPMVDFRVEVYDGQY
                     HDVDSSEMAFKIAGSLAYKDAMERARPTILEPIMKVAIQAPQEYMGDLMGDLTSRRGR
                     PAGMDQEGEDSIIRAEVPLAEMISYGSILRSITQGRGSFHMEMDHYEEVPRPLQEKLI
                     AERAKHRKVEVVEE"
     gene            3812..5161
                     /locus_tag="PLB01_16675"
     CDS             3812..5161
                     /locus_tag="PLB01_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQQ78989.1"
                     /translation="MSTATAGEGLLGSLIADGTLARPSAPAPDTLWSQRFAQRTQRIT
                     ASMIRELLKLTEKPDIISFAGGLPAPEVFPIEAIRAATERVLSDWGTSALQYTTTEGY
                     RPLRELLVRHMSRYGVKVGPENVLITSGSQQALDLIGKLLINPGDRVLTEAPTYLGAL
                     QAFNAYQADYLSVPIDDDGMDADALDEQMRGGPKFIYALPNFQNPAGVTMSLPRRRRL
                     VERASALGIPIVEDDPYGQLLYEGEHLPSLVSLDAGTHGCANGEKTFTGNVLYLSTLS
                     KTLAPGLRIAWVVAPEVVISKLVQIKQGADLHTSTFCQYVAYEVARGGFLDRHVKVIR
                     RVYGERRNAMLRALDRYAPPGVRWTKPAGGLFLWATLPEGFDTVKLLDEAIAEKVAFV
                     PGAAFYPTGGGERTMRLNFSYAAPDVIDEGIKRLCRVVEKGAERLGRRWTRTAPVAD"
BASE COUNT          824 a         1847 c         1754 g          792 t
ORIGIN      
        1 gcgcaccggg gtgggcggcg gtgcgacgcg cggcaccgca ggtcccggga gcggcggcac
       61 gcctcgtggc ggcgtcggga ccgaggcctg cgaaacgggc gcggccgaag cgaccagccc
      121 cgggatcgtc tggatgcgct ccgtcgggcg gttctcctcg tcggacaccg gggcggacgg
      181 gggcgtcacc gccggcttct tcaggccgcg ctgaacgtcc gagacgagcg tgcccggtga
      241 cgacacgggt tcgggaatct cccgctctct cttcttcgcg gcttcgcgct cggcgggcga
      301 cttcagcgcg tccgtgtcga ggctccagtc cgggatcgag ctctcgaggt tcgtcggcgc
      361 aggcccgacg agagcgggat tgagatcgct gcggccgcgc cgcgccaggt cttcgaggct
      421 cgtcgccccg cccccgaggc cggcggggcc cttcgagacg ccggcctgcg gcggcggcgg
      481 cacctcggcc agcggcgtct tcttcgactc gaccgtggac accgggccga gtttctcggc
      541 ctgcgcgacc atctcgccga ggtccttgag catctccatc tcggcatgcc gcgtgcggaa
      601 ctcggccagg gcggcgtaga agtccttccc gctctggaag cgcgccgccg ggtccttcgc
      661 gagcgcccgc gcgaggatcg ggccgaattc gcccgggatc gtcgcgtcgt acgtctcggg
      721 cggcgggaaa tcctcgtgga cgatcttgta cgaaatggac gtgaggttgt cgccgatgaa
      781 cggcttcctc ttcgtgagga gctcgtacag gacgacgccg agcgagaaga ggtcgctgcg
      841 gccgtccacc tcctcgcccg tgacctgctc gggcgacatg tagttcggcg tgccgaggaa
      901 ctggccggtc tccgtgaggc tggacgtctc gatcttcgcg atcccgaagt ccgtgatctt
      961 cacggtcccg tcgctcgtga ggatgatgat gttcgcgggc ttgacgtcgc ggtggacgat
     1021 tccgcggcgg tgggcgtagt cgagggcgtc cgcgacggac atcgcgattt ccgcgacgcg
     1081 gtcccaggag aaggcgacct tgtccttcag gagctgcttg aggttcttgc cctcgacgta
     1141 ctccatcgcg atgaaggacg tctgggtctc gacttcctcg ccgacgtcgt gaatcgtgac
     1201 gatgttcggg tgcgacagcg tgccggccgc ctgcgcctcg cgcatgaacc gttcgttgaa
     1261 ctcgcgctgc tcgttgtcgt cctccgccgt cgcccggatc gtcttgaggg cgacgcgccg
     1321 cccgatgacc gggtcccggc cgaggtagac gacccccatc gctcccttgc cgagctcgcg
     1381 ctccacctcg taacgcgcga acttacgcgg cggggggttc ggcgtgcctg gttcgggcgg
     1441 cagacggcct ccatgagcgc gtggggcgca gggccggatt ctaccagcgg gccccgggcc
     1501 cgccgaccct cgggccgctc aagacgggtg tccggagggt gcgcacaagg gcccggacct
     1561 gatgcaattg ccggggctcg acccggggct tcctctccct ataatcgcgc tcccttcaaa
     1621 cagggatcgg agggttgttc atgaaggttt atcccgcgtc cgccatccac aacgtcgctg
     1681 tcgtggggca caacggcgtc ggcaagacga cgctcgtctc cgcgctgctc cacgaagccg
     1741 gcgccacgcc gcgtttcggc cgcgtcgagg aaggcaacgc tcccacggat ttcgaccccg
     1801 aggaggtcga gcgcaagatc tcgatcgggc tcgcggtcgc gtcgctcgag tggaagaaga
     1861 agaagatcaa cctgatcgac acgccggggt acggcatctt cgtctcggag atgctctgcg
     1921 gcctgcgcgc cgccgacgcg gcgatcatcg tcgtcgacgc cgtcgcgggc gtcgaggtcc
     1981 agaccgagaa ggcctggaag gtgtgcgacg agctcggcct cccgcgcctc gtcgtcgtga
     2041 accgcatgga ccgtgaacgc gccgacggcg gccgcgtcat ggaccaactc cagaagaagt
     2101 tcggacgcgc gtgcgtctgc gtcgaggccc ccatcgggcg ggagaaggcc ttccggggcg
     2161 tcatcgacct cgtcgcaatg cagggctggc tcacggacga cgccgggaag accgcgccgg
     2221 gcacgattcc ggacgagttc gcggccgacg ccgcccagct ccacgaaacc ctcgtcgagc
     2281 tcgtggccga gggcgacgac aagctcatgg agacgttctt cgcgcagggc aacctcaccg
     2341 aggagcagat gaagcccgcg ctcaagaagg cgatccagga gcggaagatc gcccccgtcc
     2401 tcttcacggc gagcggcagc cacgagacgg gcgtcgaaaa gctcctcgac gagatcgagg
     2461 acctgctccc aaccgccgac gccttcacgt ccgtcgcaac gggcaagagc ggctcggccg
     2521 aggcgcttgc gcccgatccg gccggcccgg ccgccgcctt cgtgttcaag acgatctccg
     2581 acccgtacgc gggccggctc acgctcttcc gcgtctactc gggcacgatc aagggagacg
     2641 ggacgtactg gaacgcctcg cgcgagctcg ccgagcgctt cggccccgtg ttctcgcccc
     2701 tcggaaagac gctccagacg gtccccgaga tcccggccgg cgacatcggc ggcgtcgcga
     2761 agctgaagga gacgctcacg ggcgactccc tctcgaccaa ggacaagccg ttcgtcttcg
     2821 acaagatcgc gttccccgag gccgcaatct ccttcgcgat cgagccgaag tccaagggcg
     2881 acgaggagaa gatctcggtc gccctcgcga agctcatcga agaggacccc tcgctccact
     2941 tctcgcgcga cacggacacg cacgagttcc tcctctcggg cgccggccag ctccacgtgg
     3001 agatcgccgt cgcacgcatg aagcggaaga gcggcgtcga ggtcatcctc cacccgccca
     3061 aggttccgta ccgcgaaacg atcacgcgcg ccgcggaggc gcacggccgc cacaagaagc
     3121 agacgggcgg ccacggacag ttcgcggact gcaagatccg ggtcaagccg ctcagccgcg
     3181 gcgcggactt cgagttcttc gacgacacgt tcggcggctc gatcccgcgg aactacatcc
     3241 cggcgatcga aaagggcatc caggagatcc gcaagcgcgg cttcctcgcc ggcttcccga
     3301 tggtcgactt ccgcgtggag gtctacgacg gccagtacca cgacgtcgac tcgtcggaga
     3361 tggccttcaa gatcgccggc tcgctcgcgt acaaggacgc gatggagcgg gcgcggccga
     3421 cgatcctcga gccgatcatg aaggtcgcga tccaggcgcc gcaggagtac atgggcgacc
     3481 tcatgggcga cctcacgtcc cggcgcgggc ggcccgcggg catggaccag gagggcgagg
     3541 actcgatcat ccgcgccgaa gttccgctcg ccgagatgat ctcgtacggg tcgatcctcc
     3601 gttcgatcac gcagggacgc ggcagcttcc acatggagat ggaccactac gaggaagttc
     3661 cccggcccct ccaggagaag ctcatcgccg agcgcgccaa gcaccgcaag gtggaggtcg
     3721 tcgaggagta gggcgccgca aacgccctga tcagcccgca tcatggcggt cgggcacccc
     3781 ccggcgagac gctctcctcg ggggtgtgcc catgtcgacc gccacggccg gggaggggct
     3841 cctcggcagt ctgatcgcgg acgggaccct cgcgcgcccg tccgcccccg ctccggatac
     3901 tctctggtct caacggtttg cgcagcgcac gcagcgcatc acggcctcca tgatccgcga
     3961 gctcctgaag ctcacggaga agccggacat catcagcttc gcgggcgggc tccccgcccc
     4021 ggaggtcttc cccatcgagg cgatccgcgc cgccaccgag cgcgtcctct cggactgggg
     4081 cacgtcggcc ctccagtaca cgacgaccga aggctaccgc cccctccgcg agctcctcgt
     4141 ccggcacatg agccgctacg gcgtcaaggt cggccccgag aacgtcctca tcacgtccgg
     4201 ctcgcagcag gcgctcgacc tgatcggcaa gctcctcatc aaccccggcg accgggtcct
     4261 gaccgaggcg ccgacgtatc tcggcgctct ccaggcgttc aatgcctacc aggcggacta
     4321 tctttccgtc ccgatcgacg acgacggaat ggacgccgac gcgctcgacg aacagatgcg
     4381 cggcggcccg aagttcatct acgcgctccc gaacttccag aaccccgcgg gcgtcacgat
     4441 gagcctcccg cgccgccgcc gcctcgtcga gcgcgcctcc gcgctcggga tcccgatcgt
     4501 cgaggacgac ccgtacggcc agctcctcta cgagggcgag cacctcccgt cgctcgtgtc
     4561 gctcgacgcc gggacgcacg gctgcgcgaa cggcgagaag acgttcacgg gcaacgtcct
     4621 ctacctctcg acgctctcga agacgctcgc gcccggcctg cgcatcgcgt gggtcgtggc
     4681 gcccgaggtg gtcatctcca agctcgtcca gatcaagcag ggcgcggacc tccacacgtc
     4741 cacgttctgc cagtacgtgg cctacgaggt cgcgcgcggc ggcttcctcg accgccacgt
     4801 gaaggtcatc cgccgcgtct acggtgaacg ccgcaacgcg atgctccgcg ccctcgaccg
     4861 ctacgcccct cccggggtcc gctggacgaa gccggccggc gggctcttcc tgtgggcgac
     4921 gctcccggaa ggcttcgaca ccgtcaagct cctcgacgag gcgatcgccg agaaggtggc
     4981 cttcgtgccg ggcgccgcgt tctacccgac gggcggcggg gagcggacga tgcgcctgaa
     5041 cttctcctac gcggcccccg acgtcatcga cgagggaatc aagcggctct gccgggtcgt
     5101 cgagaagggc gccgaacgcc tcgggaggcg gtggacgcgc accgcgccgg tcgcggacta
     5161 ggtgtcgagg ctaaacaggt tgttcaccct ggatgccggt ggacggtagc cgaggga
//
LOCUS       DAOMFY010000278         5195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5593_length_5195_cov_8.964008, whole genome shotgun sequence.
ACCESSION   DAOMFY010000278 DAOMFY010000000
VERSION     DAOMFY010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5593_length_5195_cov_8.964008"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1298)
                     /locus_tag="PLB01_16680"
     CDS             complement(171..1298)
                     /locus_tag="PLB01_16680"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQQ78990.1"
                     /translation="MTVALGRKPAFIFGVWTAAGVFFASQLYFLYPITSGGREISFGR
                     ALLVNLPFYWLWALLTPAILGLARRFPLERAHWKRSLAVHAGASVALSAVQLLVAGAL
                     LYAVVQSGGTSSLSWALGSFFRLNFHANILTYAAIVALAWATETYAKYRDRELAASRL
                     QTQLVSAELGALKMQLQPHFLFNTLNAIAALLKPKPDAAESMVLQLAEFLRLTLRNTG
                     RAEVTLREELDFLERYLAIEKTRFGERLSTRFRIRSEVLDARVPNLLLQPLVENAIRH
                     GIARDAGAGRLEVSATHEGGRLLLRVADDGPGLGPSPVVEGIGLTNTRERLRHLFGDD
                     FSLSYANVPGGGFAIALAFPLVSEAETAAGEPDEVPAAYGF"
     gene            complement(1366..4350)
                     /locus_tag="PLB01_16685"
     CDS             complement(1366..4350)
                     /locus_tag="PLB01_16685"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ78991.1"
                     /translation="MRRFGLRVSLSLALLLSVASALAQSVTTATLRGRVTNEGQGLPG
                     VLVTVKSGTLQGARTASTSANGDYVFPGLPPGEYTVGFALQGFRAESRTQPVTVGQEA
                     RLDVVLSLAGVETATTVVAKSETVATSTPQAAQTFTKELTDNLPVTRTLLSSVALAAG
                     VNTNGPGSSDRIGNGTNPVVTISGGQSFDNLFTVDGAVVTDNIRGTPNNLFIEDAIAE
                     TTTSTSSVSAEFGRFTGGVVNTVTKSGGNAFSGSFRTTFTNAAWSATTPANEATPQKV
                     NPQYEATLGGPIWKDHVWFFGSGRYFDQTTSGQTSFTSLSYPIGDTEKRYQAKLTLTP
                     VANHTLTGNYLHVDEEQTNSSFGTIMDLLSLVPARQLPQEILTVNYNGVLSNTFFVEG
                     LYSRRKFTFQNAGSPYKDLIQGTLMRDLSRGRARYNSPTFCGVCDPESRDNRDFLVKG
                     TWFLSTGALGSHNIVAGYDNYAGQRKANNYQSGSNYRVFTTSTIFANNDIYPVMDGNS
                     YIYYTPITNLSKGTDALTHSVFLNDSWRVSDRLSVNLGVRWDKNHAQDSNGVVRADDS
                     AFSPRLAASYDVTGKGTLRVGASYARYVGAIQDSLVDSASDGGSPSTFIWYYQGAGAT
                     PINVNPAPGAPLVTRAQALQQVFNWFFAQGCPDLTTCRLPLGYANIPGLTTLIQGSLS
                     SPSTIEYAATVSGNVGSSLAYRVDLVRREGRDFYNNVINGSTGTGQDEFGNTFDIGYI
                     NNSNTVERNYTGLHTSLSYRSGGFNGGVNWTWSHTLGNLNGETAASGPVPNTGDTYPE
                     YRDPAWNNPYGSLATDERHRVKLFASYTLPFIPAKVGSLNVSAIQSFDTGVPYGAVGP
                     IPTRPYVTNAPNYVTPPTSVNYYFTARDAFRTDTISRTDLSLNFSTRIGGVVEIFVQP
                     QVLNVFNNHGLVALSTTVRTSTSSASCVPGSGIRCQAFDPFKTQPVQGVNWNYPAAFG
                     TARNNLDYQLPRTFTLAAGVRF"
     gene            complement(4532..4912)
                     /locus_tag="PLB01_16690"
     CDS             complement(4532..4912)
                     /locus_tag="PLB01_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="n-acetylglutamate synthase"
                     /protein_id="HQQ78992.1"
                     /translation="MSDVDFDGRRFRTVRNSAAGESGSETVFNYRQKGAVVWATYHGG
                     DVSFGTLLARAAAEGRLEMVYQHLNRNGDFRAGRCKARTEILPDGRYRLHERWVWTEG
                     AEGSGRSVTEEIPRRQRRRSGAAS"
     gene            4997..>5195
                     /locus_tag="PLB01_16695"
     CDS             4997..>5195
                     /locus_tag="PLB01_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ78993.1"
                     /translation="MDGRNDTGFSPAWWLKGAHAQTVWGRLARSRRRVPLRREALRAP
                     DGDTLFLDHAEAPVPDGAPPLL"
BASE COUNT          773 a         1681 c         1940 g          801 t
ORIGIN      
        1 tgccgcgagc gcgctccatc cggcgagcgc ggcggcggcg aggtgcagga ccgtgcggga
       61 gcgcgtcatc gcacccgaat ctcggtctcg gtcgcggccg cgtcacggga gcggggaccg
      121 cggccgcgcc tcgcgggacg gcgccgcgcg ggatcgggag gaacgcgcgg ctagaagccg
      181 tacgcggccg gcacttcgtc cggttcgccc gcggcggtct cggcctcgga gacgagcggg
      241 aacgcgagcg cgatcgcgaa acctcccccg ggcacgttcg cgtaagacag cgagaagtcg
      301 tcgccgaaca ggtggcggag gcgttcacgc gtgttcgtga gaccgatccc ctcgacgacc
      361 ggcgagggcc cgagccccgg cccgtcgtcc gccacgcgca ggaggaggcg gccgccttcg
      421 tgcgtcgcgc tcacctcgag acggccggcg cccgcgtcgc gcgcgatgcc gtggcggatc
      481 gcgttctcga cgagcggctg caggaggagg ttcggcacgc gtgcgtcaag gacctccgaa
      541 cggatgcgga agcgcgtcga gaggcgctcg ccgaaacgcg tcttctcgat cgcaaggtac
      601 cgctcgagga agtccagctc ctcgcggagc gtgacctcgg cacgtcccgt gttgcggagc
      661 gtgagccgga ggaactcggc cagttgcagg accatgctct cggccgcgtc gggcttcggc
      721 ttcaggagcg ccgcgatcgc gttgagcgtg ttgaagagga agtggggctg cagctgcatc
      781 ttgagcgcgc cgagctcggc gctcacgagc tgcgtctgga gccgcgaggc cgcgagctcg
      841 cggtcgcggt acttcgcgta cgtctcggtg gcccacgcga gcgcgacgat cgccgcgtac
      901 gtgaggatgt tcgcgtggaa attcaggcgg aagaagctcc cgagcgccca cgagagcgag
      961 ctcgtgccgc ccgactgcac gaccgcgtag aggagcgccc ccgcgacgag gagctggacc
     1021 gccgagaggg cgacgctggc cccggcgtgc accgcgaggc tccgcttcca gtgcgcccgt
     1081 tcaagcggga agcgccgcgc gaggccgagg atcgccgggg tcaggagcgc ccagagccag
     1141 tagaacggca ggttgacgag gagcgcgcgg ccgaaggaga tctcgcgtcc gccggacgtg
     1201 atggggtaga ggaagtagag ctggctcgcg aagaagacgc ccgcggccgt ccagactccg
     1261 aagatgaacg cgggtttcct cccgagggcg acggtcatgg ggacggatac gcaccggccg
     1321 gggccggggt tgccggccgg tgcgtcgggc gccgtcgccg tgggctcaga acctcacgcc
     1381 ggccgcgagc gtgaaggtgc gcggcagctg gtagtcgagg ttgttgcggg ccgtgccgaa
     1441 cgcggccggg tagttccagt tgacgccctg cacgggctgc gtcttgaacg ggtcgaacgc
     1501 ctggcagcgg attccgctgc caggaacgca ggacgccgac gaggtcgacg tccggacggt
     1561 cgtgctgagc gcgacgaggc cgtggttgtt gaagacgttc aggacctgcg gctggacgaa
     1621 gatctccacg acgcctccga tccgcgtcga gaagttcagc gagaggtcgg tgcggctgat
     1681 cgtgtccgtc cggaacgcgt cgcgggcggt gaagtagtag ttcacggacg tcggcggcgt
     1741 gacgtagttc ggggcgttcg tcacgtacgg acgcgtgggg atgggtccga ccgctccgta
     1801 gggcacgccc gtgtcgaagg actggatggc gctgacgttc agcgagccga ccttcgccgg
     1861 gatgaacggg agggtgtagg acgcaaagag cttcacccgg tggcgctcgt ccgtcgcgag
     1921 agagccgtac gggttgttcc acgccgggtc cctgtactcg gggtaggtgt cgcccgtgtt
     1981 cggtacgggc ccgctcgccg ccgtctcgcc gttcaggttg ccgagcgtgt gggaccacgt
     2041 ccagttcacg ccgccgttga agccgccgct gcggtacgag aggctcgtgt ggaggcccgt
     2101 gtagttgcgc tccacggtgt tcgagttgtt gatgtagccg atgtcgaacg tgttcccgaa
     2161 ctcgtcctgg cccgtgccgg tcgacccgtt gatcacgttg ttgtagaagt cgcggccttc
     2221 gcgccgcacg aggtcgacgc ggtaggcgag cgaggacccc acgttgccgg agaccgtcgc
     2281 ggcgtactcg atcgtcgacg gcgaggacag cgagccctgg atgagggtcg tgaggccggg
     2341 gatgttcgcg tagccgagcg gcagccggca cgtcgtgagg tccgggcagc cctgcgcgaa
     2401 gaaccagttg aagacctgct ggagcgcctg cgcgcgcgtc acgagcggcg cgccgggggc
     2461 cgggttcacg ttgatgggcg tcgcgcccgc gccctggtaa taccagatga aggtggacgg
     2521 cgagccgccg tcggacgccg agtccacgag gctgtcctgg atcgcgccga cgtaccgcgc
     2581 gtagctcgcg ccgacgcgga gcgtgccctt gccggtcacg tcgtacgacg ccgcaaggcg
     2641 cgggctgaac gcgctgtcgt ccgcgcggac gaccccgttg ctgtcctggg cgtggttctt
     2701 gtcccagcgg acgccgaggt tcaccgagag ccggtcgctc acgcgccagg agtcgttcag
     2761 gaagacggaa tgcgtgagcg cgtccgtgcc cttgctcagg ttcgtgatcg gcgtgtagta
     2821 gatgtaggag ttcccgtcca tcacgggata gatgtcgttg ttcgcgaaga tcgtggaggt
     2881 cgtgaagacc cggtagttgc tgcccgactg gtagttgttc gccttgcgct gcccggcgta
     2941 gttgtcgtag ccggcgacga tgttgtgcga gccgagagcg cccgtggaga ggaaccacgt
     3001 gcccttcacg aggaagtccc ggttgtcgcg ggactcggga tcgcagacgc cgcagaacgt
     3061 cggcgagttg taacgggccc ggccgcgcga aagatcgcgc atgagcgtcc cctggatcag
     3121 gtccttgtac ggggagcccg cgttctggaa cgtgaacttc cggcgcgagt agaggccttc
     3181 gacgaagaac gtgttcgaca ggacgccgtt gtagttgacg gtgaggatct cctgcggaag
     3241 ctggcgcgcg ggcaccaggc tgaggagatc catgatcgtc ccgaacgacg agttcgtctg
     3301 ctcttcgtcg acgtggaggt agttgcccgt gagcgtgtgg ttcgcgaccg gggtcagcgt
     3361 gagcttggcc tggtagcgtt tctccgtgtc cccgatcggg tacgacaggc tcgtgaagct
     3421 cgtctggccc gacgtcgtct ggtcgaagta ccggccggag ccgaagaacc agacgtggtc
     3481 cttccagatg ggcccgccga gcgtcgcctc gtactgggga ttcaccttct gcggcgtcgc
     3541 ctcgttcgcg ggcgtcgtcg ccgaccacgc ggcgttcgtg aacgtcgtcc ggaaagagcc
     3601 cgagaacgcg ttgccgcccg acttcgtgac ggtgttcacg acgccgcccg tgaagcggcc
     3661 gaactcggcg gacaccgacg aggtggacgt cgtcgtctcc gcgatcgcgt cctcgatgaa
     3721 gaggttgttc ggcgtgccgc gaatgttgtc ggtcacgacg gcgccgtcca ccgtgaagag
     3781 gttgtcgaag gactggccgc ccgagatcgt cacgacgggg ttcgtcccgt tcccgatccg
     3841 gtcgctcgaa cccgggccgt tcgtgttcac gccggcggcg agcgcgacgg acgagagcag
     3901 cgtgcgcgtg accggtaggt tgtccgtgag ctccttcgtg aacgtctgcg ccgcctgcgg
     3961 cgtggacgtc gcgacggtct cggacttcgc gacgacggtc gtcgccgtct cgacgcccgc
     4021 gaggctgagc acgacgtcga gccgcgcctc ctgcccgacg gtgaccggct gcgtgcgact
     4081 ctccgcccgg aacccctgca gcgcgaaacc caccgtgtat tcgccgggcg gcaggcccgg
     4141 gaacacgtag tcgccgttcg cagacgtcga ggccgtgcgg gccccctgca gggtgcccga
     4201 tttcaccgtc acgaggacgc cggggagtcc ctgtccttcg ttcgtgacgc gtccgcgaag
     4261 cgtcgcggtc gtcacgctct gggcgagggc ggacgcgacg gagaggagca gggcgaggga
     4321 gagagagacg cggagtccga atcggcgcat gcacatcctc cttggcggcg cgaagacccc
     4381 gcaaaaacgg gactcgcgcc tcgacggcca aggtggggcg agccggagag tctttcgacg
     4441 actctgggac gggcgcccgt gggccgtgcc cggcgtcgcg cccccgggga cggagccggc
     4501 ccgcggcctg cgggtccggg aacccggagc ctcacgaggc cgcgccggat cggcgccgct
     4561 gccgccgcgg aatctcctcc gtgaccgagc ggccggagcc ctcggcccct tccgtccaca
     4621 cccagcgttc gtggagccgg taccgcccgt ccgggaggat ctccgttcgc gccttgcacc
     4681 ggcccgcgcg aaagtcgccg ttccggttga gatgctggta gaccatctcg agccgccctt
     4741 ccgcggccgc ccgcgcgaga agcgtcccga acgagacgtc accgccgtga tacgtcgccc
     4801 agacgacggc gcccttctgc cggtagttga agacggtctc ggaaccggac tcgccggcgg
     4861 cggaattgcg gacggtccgg aagcgccggc cgtcgaagtc gacatcgctc atgccccgga
     4921 caatagaatc cccgctccgc ggacggcgct cggcggggac gaacgcagga tccggcggga
     4981 cgaaaggctc ggctcgatgg acgggagaaa cgacacgggc ttttcgcccg cgtggtggct
     5041 caagggcgcg cacgcgcaga cggtctgggg ccggctcgcc cgctcccggc ggcgggtgcc
     5101 gctccggcgc gaggcgctcc gggcgcccga cggcgacacg ctgttcctcg atcacgcgga
     5161 ggcgcccgtc cccgacggcg cgccgccgct tctga
//
LOCUS       DAOMFY010000279         5195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5594_length_5195_cov_6.442607, whole genome shotgun sequence.
ACCESSION   DAOMFY010000279 DAOMFY010000000
VERSION     DAOMFY010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5594_length_5195_cov_6.442607"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..439
                     /locus_tag="PLB01_16700"
     CDS             59..439
                     /locus_tag="PLB01_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78994.1"
                     /translation="MSLRRLSTRAARLALAIPLAFFGGPALAAGASRTFTLAGSCAVP
                     AAAQTVSANVTIVSPAAAGDLVIYPATLVSPPAVSTISFRAGRTRANNTHLALSSDGT
                     GRVTVRNNTNGSLNLVLDVNGYYR"
     gene            508..2019
                     /locus_tag="PLB01_16705"
     CDS             508..2019
                     /locus_tag="PLB01_16705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQQ78995.1"
                     /translation="MAPDATTAAERTARATAPADAPLLRDFVGGAWVDSRATSTIDVY
                     NPATGAVIARSPLGTRADVDAAVAAAKAAFPGWRATPVVERARAMFAFKNRLEEHFEE
                     ISRIVTTEHGKTLDESRGSVRRGIEAVEVACGMPSMMMGYGLEDIARGLDCDVHRQPM
                     GVFAAIAPFNFPAMVPLWFLPFAVATGNTFVVKPSELVPLSQKRMHELLSECVPPGVV
                     NLVNGGVDVVNGLCEHPDVKGVSFVGSTPVAKHVYRTASHAGKRVQALGGAKNFIVVM
                     PDADLDKSIGIVSESCYGCAGERCLAGSIVVTVGGASHHGDVRERLVAAARAIRVGDG
                     LEPGITMGPVIREAARQRVLAYVEKGLAEGAELVLDGRGHVVKGRENGFFLGPTVFDK
                     VSPKSAIGREEIFGPVLSLMNVPDLASALALLHDHPQANATSIFTGSGKSARTFASQA
                     PPAMVGVNVGVAAPMSFFPFGGAKESFFGDVKAHGRDGIAFYTDQKVVIERWF"
     gene            2082..2945
                     /locus_tag="PLB01_16710"
     CDS             2082..2945
                     /locus_tag="PLB01_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQQ78996.1"
                     /translation="MRKVTIGLIQTRNPVSDPKASIARVQKAAMAATLPFVHEAGKKG
                     VQILGLQEIFNGPYFCPSQDAKWYDAAEPVPGPTTEAIAKVAKKYRMAVVVPVYERED
                     AGVYYNTAAVYDTDGAYLGKYRKNHIPHTSGFWEKYFFRPGNMGYPVFQTRHAKVGVY
                     ICYDRHFPEGARALGLNGAEVVFNPSATVAGLSQYLWKLEQPAHAVANGYFVAASNRV
                     GTEAPWNIGRFYGSSYVVDPRGNFLATASEDKDELLVATCDLDMIEEVRRTWQFFRDR
                     RPETYGPLVAL"
     gene            2989..4374
                     /gene="hydA"
                     /locus_tag="PLB01_16715"
     CDS             2989..4374
                     /gene="hydA"
                     /locus_tag="PLB01_16715"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HQQ78997.1"
                     /translation="MNRLLIRGGRIVTAVDDYIGDVLVEGEKISAVFAHGAAVPNDVE
                     TLDAAGKLVIPGGIDAHTHLDMPFGGTTSADDFESGTIAAAHGGTTSIVDFAIQYKGQ
                     TLREARDAWMKKAEGKAAIDYGFHMIITDLPDSVEKEMDVLVKEGVTSFKLFMAYRGV
                     LMLDDGSIFRALLKTGENGGTICMHAENGDVIDVLVRRALAEGHTAPKWHALTRPPRA
                     EAEATYRAICLAEMANVPLYVVHLSAAEALEKIAEARDRGLPMHAETCPQYLFLSYDN
                     YEEPGFEGAKYVMSPPLRAKGNEDALWRGLKMNDLQAVSTDHCPFCMKDQKVLGKDDF
                     SKIPNGAPGIETRMSLLWDGGVVKGRISPNRFVELTSTSPAKIFGLFPKKGTIAPGSD
                     ADIVVFDPNRKVTLSAKTLHQRVDYTPYEGRVVQGAADVVISRGEIIVQNGTFKGKAG
                     RGDFLRRKTRA"
     gene            complement(4375..>5195)
                     /locus_tag="PLB01_16720"
     CDS             complement(4375..>5195)
                     /locus_tag="PLB01_16720"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQQ78998.1"
                     /translation="LVALLGITSCGGATLPAIDTSPPPDPPPPASDDVPGAPECDTVL
                     GTAAAVQQATASAAPGTTVCIADGTYANFNLALVGNGTESAPIRVAAQHPGGVILTGA
                     QYVGLSGQFAAWAGALLPPDRPVVLVTEDEAGAAEAATRLARVGLENVAGYLDGGVAA
                     WDRAGLPVARLEQIDVEELKARRQEEPGLRILDVRRPGEYAAGHVPGAIPVPLDELAR
                     EIDVLPSQGRVAIICASGYRSSVAGSLLLHRKGAEPINVVGGTNAWVAAGFPVE"
BASE COUNT          838 a         1803 c         1787 g          767 t
ORIGIN      
        1 ggtagtaacc tgacacgcgg atcttcaatg cgatgacgcg cgaggtcggt caatgagaat
       61 gagcctgcgc cgcctctcga cgcgggccgc ccggctggcg ctcgcgatcc ctcttgcgtt
      121 cttcggcggg cccgcgctcg ccgccggggc ctcgcgcacg ttcacgctcg ccggctcgtg
      181 cgccgtcccg gccgccgcgc agaccgtttc ggcgaacgtc acgatcgtga gcccggccgc
      241 cgccggcgac ctcgtgatct accccgcgac gctcgtgtct ccccctgccg tctcgacgat
      301 cagcttccgg gccggcagga cgcgggcgaa caacacccac ctcgcgctct cgagcgacgg
      361 caccggccgc gtgaccgtgc ggaacaacac gaacgggtcg ctgaacctcg tcctcgacgt
      421 gaacgggtac taccggtagg cgcgcccagg gccgctgaga ttcgctctcc ccgccttaga
      481 atggcgcttc cccctcagga ggtgcccatg gctcccgacg cgacgaccgc cgccgaacgc
      541 accgctcgtg ccaccgcgcc cgcggacgct cccctccttc gcgacttcgt cggcggcgcg
      601 tgggtcgact cccgcgcgac gtccacgatc gacgtctaca accccgcgac cggcgccgtc
      661 atcgcgaggt ctccgctcgg gacgcgcgcc gacgtggacg cggccgtcgc cgctgcgaag
      721 gcggcgtttc ccggatggcg cgcgacaccc gtcgtggaac gtgcgcgggc gatgttcgcg
      781 ttcaagaacc ggctcgagga gcacttcgag gagatctccc gcatcgtgac gaccgagcac
      841 gggaagacgc tggacgagtc gcgcgggtcc gtccgccgcg gcatcgaggc cgtcgaggtc
      901 gcatgcggga tgccctcgat gatgatgggc tacggcctcg aagacatcgc gcgcgggctg
      961 gactgcgacg tccaccgcca gccgatgggc gtcttcgcgg caatcgcgcc gttcaacttc
     1021 ccggccatgg tcccgctctg gttcctcccc ttcgcggtcg cgaccggcaa cacgttcgtc
     1081 gtgaagccgt ccgagctcgt cccgctctcg cagaagcgca tgcatgagct cctctcggag
     1141 tgcgtcccgc ccggagtcgt gaacctcgtg aacggcggcg tcgacgtcgt gaacgggctc
     1201 tgcgagcacc cggacgtcaa gggcgtctcg ttcgtgggat cgacgcccgt cgcaaagcac
     1261 gtctaccgga cggcctcgca cgccggcaag cgcgtccagg ccctcggcgg cgcgaagaac
     1321 ttcatcgtcg tgatgccgga cgcggacctc gataagtcga tcggcatcgt ttcggagtcc
     1381 tgctacggct gcgcgggcga gcgctgcctc gcgggctcga tcgtcgtcac cgtcggcggc
     1441 gcgagccacc acggggacgt ccgcgagcgc ctcgtcgcgg ccgcgcgggc gatccgggtg
     1501 ggcgacggcc tcgagccggg aatcacgatg gggccggtca tccgcgaagc ggcgcggcag
     1561 cgcgttctcg cgtacgtcga gaagggcctc gcggagggcg ccgagctcgt cctcgacggg
     1621 cgcggccacg tcgtgaaggg ccgcgagaac ggcttcttcc tcgggccgac ggtcttcgac
     1681 aaggtctcgc cgaagtccgc gatcggccgc gaggagatct tcgggccggt tctctccctc
     1741 atgaacgtcc cggacctcgc gtccgcgctc gcgctgctcc acgaccaccc gcaggcaaac
     1801 gcgacgtcca tcttcacggg gagcgggaag tccgcgcgga cgttcgcgtc gcaggcgccg
     1861 cccgccatgg tcggcgtgaa cgtgggcgta gcggcgccga tgtcgttctt cccgttcggc
     1921 ggggcgaagg agagcttctt cggcgacgtg aaggcgcacg gccgcgacgg catcgcgttc
     1981 tacacggacc agaaggtcgt catcgagcgt tggttctagg aagagaagaa gaagagaaga
     2041 tttcttagaa ggttaagaga agagacggga gggccgggcg aatgaggaag gtcactatcg
     2101 ggctgatcca gacgcgcaat ccggtctcgg atccgaaagc ttccatcgct cgggtccaga
     2161 aggcggcgat ggcggccacg ctgcccttcg tgcacgaggc cgggaagaag ggcgtgcaga
     2221 tcctcgggct gcaggagatc ttcaacggcc cgtacttctg cccgtcgcag gacgcgaagt
     2281 ggtacgacgc cgccgagccc gtgcccggtc ccacgaccga ggcgatcgcg aaggtcgcga
     2341 agaagtaccg catggcggtc gtcgtgcccg tctacgagcg cgaggacgcc ggcgtctact
     2401 acaacaccgc ggccgtctac gacacggacg gcgcgtacct cggcaagtac cgcaagaacc
     2461 acatcccgca cacctccggc ttctgggaga agtacttctt ccggccgggc aacatgggct
     2521 accccgtctt ccagacgcga cacgcgaagg tcggcgtcta catctgctac gaccgccact
     2581 tcccggaggg tgcgcgcgcg ctgggactga acggcgccga ggtcgtcttc aacccgtccg
     2641 cgacggtcgc gggcctctcg cagtacttgt ggaagctcga gcagcccgcg cacgcggtcg
     2701 cgaacggcta tttcgtggcg gcgagcaacc gcgtcggcac cgaggcgccc tggaacatcg
     2761 ggcgtttcta cgggtcctcc tacgtcgtcg acccgcgcgg caacttcctc gcgaccgcga
     2821 gcgaggacaa ggacgagctc ctcgtcgcga cctgcgacct cgacatgatc gaggaggtcc
     2881 gccgcacgtg gcagttcttc cgggacaggc ggcccgagac gtacgggccg ctggtggcgc
     2941 tgtgagacgt tcgaagagca cgaaagatct tcttagaagg gaagaaagat gaatcggctg
     3001 ttgatccgcg ggggccggat tgtcacggcg gtcgacgact acatcggtga cgtactcgta
     3061 gagggcgaaa agatttccgc cgttttcgct cacggcgccg ccgttcccaa cgacgtcgag
     3121 accctcgacg cggccggcaa gctcgtgatc ccgggcggca tcgacgcgca cacgcacctc
     3181 gacatgccgt tcggcgggac gacgtcggcg gacgacttcg agtccgggac gatcgccgcc
     3241 gcgcacggcg ggacgacgtc catcgtggac tttgcgatcc agtacaaggg ccagacgctg
     3301 cgcgaggcca gggacgcctg gatgaagaag gccgagggca aggccgcgat cgactacggc
     3361 ttccacatga tcatcacgga ccttccggac tccgtcgaga aggagatgga cgtcctcgtg
     3421 aaggagggtg tgacgtcgtt caagctcttc atggcgtacc gcggcgtcct catgctggac
     3481 gacgggtcca tcttccgcgc gctgctcaag accggggaga acggcggaac gatctgcatg
     3541 cacgccgaga acggcgacgt catcgacgtc ctcgtacggc gcgcgctcgc cgagggccac
     3601 acggcaccga agtggcacgc cctgacgcgg cctccgcgcg ccgaggccga ggccacgtac
     3661 cgcgcgatct gcctcgccga gatggccaac gtcccgctct acgtcgtcca cctttcggcc
     3721 gcagaggcgc tcgagaagat cgccgaggcg cgggaccgag gcctcccgat gcacgccgag
     3781 acgtgcccgc agtacctctt cctctcgtac gacaactacg aggagcccgg gttcgagggc
     3841 gccaagtacg tcatgtcgcc gccgctccgc gcgaagggca acgaggatgc gctctggcgc
     3901 ggccttaaga tgaacgacct gcaggccgtg tcgacggacc actgcccgtt ctgcatgaag
     3961 gaccagaagg tcctcgggaa ggacgacttc tcgaagatcc cgaacggcgc gcccggcatc
     4021 gaaacgcgca tgtcgctcct ctgggacggc ggcgtcgtga agggacgcat ctcgccgaac
     4081 cgcttcgtcg agctgacctc cacgtcgccc gcgaagatct tcggcctctt ccccaagaag
     4141 gggacgatcg ccccgggctc ggacgccgac atcgtcgtct tcgacccgaa ccggaaggtc
     4201 acgctgagcg cgaagacgct ccaccagcgc gtcgactaca cgccgtacga gggccgggtc
     4261 gtccagggcg cggcggacgt cgtgatctcg cggggcgaga tcatcgtgca gaacggaacg
     4321 ttcaagggga aggccggaag aggggatttt cttagaagga agacgcgggc gtagctactc
     4381 gacgggaaat cccgcggcga cccaggcgtt cgtgccgccg acgacgttga tcggctcggc
     4441 gcccttccgg tggagcagca ggctgcccgc gacgctcgac cggtagccgc tcgcgcagat
     4501 gatcgcgacg cggccctggg acggcaggac gtcgatctcg cgggcaagct cgtcgagcgg
     4561 gacggggatc gcgcccggca cgtgacccgc ggcgtactcc cccggccgcc tcacgtcgag
     4621 gatcctgagg ccaggctctt cctgacgccg cgccttcagc tcctcgacgt cgatctgctc
     4681 gagccgcgcg accggtaagc ccgcgcggtc ccacgcggcg acgccgccgt cgaggtagcc
     4741 ggccacgttc tcgagtccga cgcgcgcgag gcgcgtcgcg gcctcggcgg cgcccgcctc
     4801 gtcctccgtg acgaggacga cgggccggtc cggcgggagc agcgcccccg cccaggccgc
     4861 gaactgcccg gagaggccca cgtactgcgc gcccgtgagg atcactccgc cgggatgctg
     4921 cgcggcgacg cggatcggcg cgctctcggt gccgttgccc accagggcga ggttgaagtt
     4981 cgcgtaggtt ccgtcggcga tgcacacggt cgtgcccggt gccgcgctcg cggtggcctg
     5041 ctgcacggcc gctgccgtgc ccagcacggt gtcgcactcc ggcgcgcccg gcacgtcgtc
     5101 gctcgcgggc ggcggcggat ccggcggagg agatgtgtcg atcgcgggca gggtcgcgcc
     5161 gccacacgag gtgatgccca ggagcgcgac gagcg
//
LOCUS       DAOMFY010000280         5192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5597_length_5192_cov_6.228343, whole genome shotgun sequence.
ACCESSION   DAOMFY010000280 DAOMFY010000000
VERSION     DAOMFY010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5597_length_5192_cov_6.228343"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLB01_16725"
     CDS             <1..395
                     /locus_tag="PLB01_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ78999.1"
                     /translation="ATGRGRTGWVLAGGLAVAGLAWGPAILAESARVKAELAEEVRLG
                     LLPPEDAEALRFPWTRGFEKRFGRTDERREYVRSALLLAAARHQQRHRTGEAVRLRQL
                     EVLTFRTRLRRTQDARSARFERAESPEV"
     gene            complement(408..1769)
                     /locus_tag="PLB01_16730"
     CDS             complement(408..1769)
                     /locus_tag="PLB01_16730"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ79000.1"
                     /translation="MSPVRVPPAPGAPLPFSPPRFERTVLPSGLAVRAARWGSRPTVA
                     ASIVFPGAGSTADPPGREGTADVTGDTFLGGTRRKTARQLAEALDDLAAVADVSAGTD
                     STVARLYVLESDLDAGLALFAEILTEATFPEEEFDKGRRRLIAALQEQRSEPDFLARE
                     RLYDRLYPGHPYGTVSPTEKGLLALTRDDVTEFARRRLSFAGATLVLAGAADPAALLA
                     AAERSFGGLPATSGEPGFPVPPPPRVEGFSIHLVNRPGSVQTNLLFARPAIARRSPRF
                     PAAVVANQSLGGGASSRLFHVLREERSLTYGAYSSLATRVLGGHFGASIDCRTEVTRE
                     ALGGLLDLIRAFAAEGPAVEEHERSKNYLLGSFPIPRETPGGVVQDEITRILHGLPED
                     EFATWRDRIRAVTREDARDAAAELFDPATGVVAAVGDAGKIRPILEARGETTLWDADG
                     PRE"
     gene            complement(1766..2947)
                     /locus_tag="PLB01_16735"
     CDS             complement(1766..2947)
                     /locus_tag="PLB01_16735"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ79001.1"
                     /translation="MVTVDVTYDVGSRVELPGKTGFAHLFEHLMFEGSENVGKGEHFR
                     LVASAGGTMNGTTSEDRTNYYETLPAECLDLGLFLEADRMRALVLTQEKLDNQREAVK
                     EEKRLRIDNQPYAPSFETADALAYDAFAYKHSVIGSMDDLGAASLDDARRFYEMYYAP
                     GNALLAITGDVEPAEAFRRARKFFDDVPARGKPAPFAVVEPPPTAERRAEVSDPHAAV
                     PALHLNFKVADRRHPDLFALSYAEKVLGAGESGRLWRRLVKDDELALSLTVSLDERRG
                     PSLFRFFAIARPGVAPERLEAEILADVARLAAEGPTAREMERVRRVITAGAIRATQTT
                     QAVAFLLSEYGVYDGDPSLWRRDLDEVLAVEAEHVRDAVQRWLVAARRSVVAVRPTEA
                     A"
     gene            3087..4130
                     /locus_tag="PLB01_16740"
     CDS             3087..4130
                     /locus_tag="PLB01_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79002.1"
                     /translation="MKGLLFAALAAVLLGPPAASAAADDPPKDGASPPKFRFEAVLHD
                     YEPLENRETRVAELYWAGVTADWTRGAFSAHAEARATEGTFRPYFDGSFWLEEGWVAV
                     RTPAGGVRVGKVAPLVGLADETFPGTLFSLNGVTRNPGWGAQLDGEARRGLDTIAWTA
                     AFVAENDHVGWEEEGRDVESDPARILRDGLSARTSYTLSKVLWWVRPGVSAATARLVP
                     KDGSDEFRRSDFAADLTVAFGPLSLFVEGFWRTSGRPCAPGAPCRFGYDDSNAALAGF
                     RAEFPSVTWRYTWSRWHYLGAEAVEEIHMPGAVWVPVKGISASIEYSARHLRTLAGSS
                     TVKAFQLGLAASF"
     gene            4186..>5192
                     /locus_tag="PLB01_16745"
     CDS             4186..>5192
                     /locus_tag="PLB01_16745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ79003.1"
                     /translation="MKKRILAVLAAVVVVAGGAWFLVARKGSAEPKYRKGKIDKGDVV
                     ATVTATGTLSAVTTVKVGSQVSGIIAKLHVDFNSAVKKGQLLAELDPTPFQQSVEQRR
                     ADLEKSKVELRNTELSLTRAKNLTKQQLLAQSELDAALTNRDAAAAAVAQSEAALKQA
                     LTNLSYTKIASPIDGVVVDRQYDVGQTVAASFQAPVIFTIAQDLTKMQVLTNIDEADV
                     GRVRVEQVASFTVDAFPEQSFKGSVSQIRLSPQTVQNVVTYPVILDVPNQDLKLKPGM
                     TANVQIPVDARRDVLRVPNAALRFRPDPADVAGSDKAKGGAPATASAPAASAPASAAG
                     AA"
BASE COUNT          724 a         1798 c         1963 g          707 t
ORIGIN      
        1 aggccacggg ccgcgggcgg accggatggg tgcttgcagg cggcctcgcg gtggcgggcc
       61 tcgcgtgggg ccccgcgatc ctcgccgaaa gcgcccgggt caaggcggaa ctcgccgagg
      121 aggtccgact cggcctcctt cctcccgagg acgccgaggc gctgcggttt ccgtggacgc
      181 gcggtttcga gaagcgcttc gggcggacgg acgagcggcg cgagtacgtg agaagcgcgt
      241 tgctcctcgc ggccgcgcgg caccagcagc ggcaccggac cggcgaggcg gtgcgtctca
      301 ggcagctcga ggtgctcacg ttccgcacgc gcctcaggcg cacgcaggac gcacggtcgg
      361 cccgcttcga gcgcgccgag tcgcctgagg tctagacccg gaattctcta ctcccgcggg
      421 ccgtccgcgt cccagagcgt cgtctcgccg cgcgcttcga ggatcggccg gatctttccg
      481 gcgtcgccca cggccgcgac gacgccggtg gccgggtcga acagctctgc ggcggcatcg
      541 cgggcgtcct cgcgcgtcac ggcgcggatc cggtcccgcc acgtcgcgaa ctcgtcctcg
      601 ggcaggccgt gcaggatgcg cgtgatctcg tcctggacga cgccgcccgg agtctcgcgc
      661 gggatcggga acgagccgag gagatagttt ttcgagcgct cgtgctcttc gacggcgggt
      721 ccttcggcgg cgaaggcgcg gatgaggtcg aggaggccgc cgagcgcctc ccgggtgact
      781 tcggtccggc agtcgatcga cgcgccgaag tggcccccga ggacgcgggt cgcgagcgac
      841 gagtaagcgc cgtacgtgag cgagcgctcc tcgcggagga cgtggaagag acgcgaagac
      901 gcgccgccgc cgagggactg gttcgccacg acggcggcgg ggaagcgcgg gcttcgtcgg
      961 gcgatcgcgg gccgcgcgaa gaggaggttc gtctggacgg agcccggccg gttcacgagg
     1021 tggatggaaa agccctcgac ccggggcgga ggcgggacgg ggaagcccgg ctcgccggac
     1081 gtcgccggca ggccgccgaa ggagcgctcc gccgccgcga ggagcgccgc gggatcggct
     1141 gcgcccgcga ggacgagcgt cgcgcccgcg aacgacagcc ttcgccgggc gaactcggtg
     1201 acgtcgtcgc gcgtgagcgc gaggaggccc ttctcggtgg gtgagacggt tccgtacggg
     1261 tgaccggggt agagccggtc gtagaggcgc tcgcgtgcga ggaagtcggg ctcggagcgc
     1321 tgctcctgca gggccgcgat gagccgccgg cgccccttgt cgaactcctc ctcggggaac
     1381 gtggcctcgg tcaggatctc ggcgaagagc gcgagcccgg cgtcgaggtc gctctcgagg
     1441 acgtagaggc gcgcgaccgt cgagtcggtg ccggccgaga cgtccgcgac ggcggcgagg
     1501 tcgtcgaggg cctcggcgag ctgccgcgcg gtcttcctgc gggtgccgcc gaggaacgtg
     1561 tctccggtga cgtcggcggt cccctcccgg cccggcgggt ccgccgtgga gccggcgccc
     1621 gggaacacga tcgaggccgc gacggtggga cggctgcccc agcgggcggc gcgcacggcg
     1681 agtcccgacg gcaggaccgt cctctcgaag cggggcggcg agaacggcag cggcgcgccg
     1741 ggcgccggcg ggacacggac cgggctcacg cggcctccgt ggggcgcacg gcgacgacgg
     1801 agcgccgcgc ggccaccagc cagcgctgga ccgcgtcgcg cacgtgctcg gcctcgacgg
     1861 cgaggacctc gtcgaggtcc cgccgccaga ggctcgggtc gccgtcgtag acgccgtact
     1921 cggagaggag gaacgccacg gcctgcgtcg tctgcgtcgc ccggatggcc ccggcggtga
     1981 tgacgcgccg gacgcgctcc atctcgcggg cggtcgggcc ctcggcggcg aggcgcgcga
     2041 cgtccgcgag gatctccgcc tcgaggcgct cgggagcgac gcccggccgc gcgatcgcga
     2101 agaaccggaa gagcgacggg ccccggcgct cgtcgagcga gacggtcagc gacagggcga
     2161 gctcgtcgtc cttcacgagg cgccgccaga gccgcccgct ttcgcccgcg ccgaggacct
     2221 tctcggcgta cgagagcgcg aagaggtcgg ggtgacgccg gtcggccacc ttgaagttca
     2281 ggtggagcgc ggggacggcc gcgtgcgggt cggagacctc ggcgcggcgc tccgcggtcg
     2341 gcggcggctc gacgacggcg aaaggcgcgg gcttcccgcg ggccgggacg tcgtcgaaga
     2401 acttgcgggc gcgccggaaa gcctcggcgg gctcgacgtc gcccgtgatc gcgaggagcg
     2461 cgttccccgg ggcgtagtac atctcgtaga aacgccgcgc gtcgtcgagc gatgcggcgc
     2521 cgaggtcgtc catcgacccg atcacggagt gcttgtaggc gaacgcgtcg taggcgagcg
     2581 cgtcggccgt ctcgaaggac ggcgcatacg gctggttgtc gatgcgcagg cgtttctctt
     2641 ccttcacggc ctcgcgctgg ttgtccagct tctcctgcgt gagcacgagg gcgcgcatcc
     2701 ggtcggcctc gaggaagagg ccgaggtcga ggcactccgc cgggagcgtc tcgtagtagt
     2761 tcgtccggtc ctcggacgtc gtgccgttca tcgtgccgcc ggcggaggcg accaggcgga
     2821 agtgctcgcc cttgccgacg ttctcggagc cctcgaacat gaggtgctcg aagaggtggg
     2881 cgaaccccgt cttgccggga agctccacgc ggctgccgac gtcgtacgtg acgtccaccg
     2941 tgaccagagg gattccgcgc tggggcgaga cgacgacggt caggccgttc gcgaggcgct
     3001 cgacgtgcgc ggggatctcg agttcgaagg aaatgggacc tccaggcgtc ccgcggccgg
     3061 cggggcccgt aatatacgcg gctgtcttga agggtctcct cttcgccgcg ctcgcggccg
     3121 tcctcctggg ccccccggcg gcctccgcgg ccgcggacga ccctccgaag gacggcgcct
     3181 cccccccgaa gttccggttc gaggccgtgc tccacgacta cgagcccctc gagaaccgcg
     3241 agacgcgcgt cgccgagctc tactgggccg gggtgacggc cgactggacg cggggcgctt
     3301 tctcggcgca cgccgaggcg cgcgcgacgg aggggacgtt ccggccctat ttcgacggca
     3361 gcttctggct ggaggagggc tgggtggcgg tccggactcc cgcgggcggc gtccgggtgg
     3421 gcaaggtcgc gccgctcgtg ggtctcgcgg acgagacgtt ccccggaacc ctcttctcgc
     3481 tcaacggcgt cacgcgcaac cccggctggg gcgcgcagct ggacggcgag gcacgccggg
     3541 gcctcgacac gatcgcgtgg acggcggcct tcgtcgccga gaacgaccac gtggggtggg
     3601 aggaggaggg gcgcgacgtc gagtccgatc cggcgcggat cctgcgcgac ggcctctcgg
     3661 cccggacctc gtacacgctg agcaaggtgc tctggtgggt ccggcccggc gtctcggccg
     3721 cgacggcgcg gctcgtcccg aaggacggct cggacgagtt ccggaggagc gacttcgcgg
     3781 cggacctcac ggttgccttc gggcccctct ctctcttcgt ggaggggttc tggaggacgt
     3841 cgggccggcc gtgcgctccc ggagccccgt gccgcttcgg ttacgacgac tccaatgcag
     3901 ccctcgcggg cttccgggcc gaattcccgt ccgtgacctg gcggtacacc tggtcgcggt
     3961 ggcattacct cggggccgag gccgtcgagg agatccacat gcccggcgcc gtctgggtcc
     4021 cggtcaaggg catctcggcc tcgatcgagt acagtgcgag gcacctgcgg acgctcgccg
     4081 gatcgagcac cgtcaaggcg ttccagctgg gtctggcggc atctttctga gagctccggt
     4141 caaccccatg gtgatttccg gcgtccaacg ccgtttgagg actgcatgaa gaagagaatc
     4201 ctggccgtgc tcgcggccgt cgtcgtggtc gcgggaggcg cctggttcct ggtcgcccgc
     4261 aagggctccg cggagccgaa ataccggaag ggaaagatcg acaagggcga cgtggtcgcg
     4321 accgtgacgg cgaccggcac tctttcggcg gtcacgaccg tcaaggtcgg cagccaggtc
     4381 tcggggatca tcgccaagct ccacgtcgac ttcaattcgg cggtcaagaa ggggcagctg
     4441 ctcgccgagc tcgacccgac gcccttccag cagagcgtcg agcagcggcg cgccgacctc
     4501 gagaagtcga aggtggagct gcgcaacacg gagctttccc tcacgcgcgc gaagaacctc
     4561 acgaagcagc agctcctcgc gcagtccgag ctcgacgccg ccctgacgaa ccgcgacgcc
     4621 gcggcggcgg ccgtcgcgca gagcgaggcc gcgctcaagc aggcgctcac gaacctctcg
     4681 tacacgaaga tcgcgtcgcc gatcgacggc gtcgtcgtcg accgccagta cgacgtcggc
     4741 cagacggtcg cggcgtcctt ccaggcgccc gtcatcttca cgatcgccca ggacctcacg
     4801 aagatgcagg tcctcacgaa catcgacgag gccgacgtcg gccgcgtccg cgtggagcag
     4861 gtcgcgagct tcacggtcga cgcgttcccc gagcagtcct tcaagggctc ggtctcgcag
     4921 atccggctct cgccccagac cgtccagaac gtcgtgacgt accccgtcat cctcgacgtg
     4981 ccgaaccagg acctgaagct caagcccggc atgacggcga acgtccagat tcccgtcgac
     5041 gcgcgccgcg acgtcctgcg cgttccgaac gccgcgctgc gcttccggcc cgatccggcg
     5101 gacgtcgcgg gaagcgacaa ggccaagggc ggagccccgg cgacggcgtc cgccccggcg
     5161 gcctccgccc cggcgtcggc ggccggcgcg gc
//
LOCUS       DAOMFY010000281         5155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5660_length_5155_cov_7.116275, whole genome shotgun sequence.
ACCESSION   DAOMFY010000281 DAOMFY010000000
VERSION     DAOMFY010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_5155_cov_7.116275"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLB01_16750"
     CDS             complement(<1..930)
                     /locus_tag="PLB01_16750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79004.1"
                     /translation="MTLVAGTKLGPYEILGQIGAGGMGEVYKARDPRLNRDVAVKLLP
                     VDFLEGEERKQRFEREAKLLAALNHPNIAAVYSFEEIPSSSSSSVSVLVMELVDGETL
                     RERITAGALPVRKAVGLGAQIARGLAAAHESGIVHRDLKPENVVLTKDGRAKILDFGL
                     AKQRAVTAGEDTKSPTMEKATDAGTLLGTVGYMAPEQVRGLPADARSDIFAFGCVLFE
                     MLTGRRAFKGDSAIETMNAILKEEPAEPDVSGAKIPPELDRLVRHCLEKNPAERFQSA
                     RDLAFDLEASAGSSASGRAAALAPPRGGRRWAPA"
     gene            complement(927..3344)
                     /locus_tag="PLB01_16755"
     CDS             complement(927..3344)
                     /locus_tag="PLB01_16755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ79005.1"
                     /translation="MTLTAGTRLGAYEILSPLGKGGMGEVYRAKDVRVDRVVALKVLP
                     EEFFEGEERRQRFEREAKMLASLNHPGIATLYSFEEIPSSSFSSSPMSRHVLVMELVE
                     GEGLDARAAAGPLPLEVTISLARQVAEALEAAHEKGIVHRDLKPANVMVTKGGRVRVL
                     DFGLAKVAASGAGAQVDSALATDMRTQEGVVVGTVPYMSPEQAQGRPLDHRTDIFSLG
                     VILYELATGQRPFRGDSSAELLSSILRDTPRPPGELRPGLPAGLDRLIGRCLEKDRDR
                     RYATAHDLAVDLGELQRDPTSPTRPASAAPFRTSKRLVLLGVAGFAVLLAAGAFLVLR
                     RAGRGASIGSVAVLPFENASGDPSIEYLSDGISESLISTLSRLPGLRVISRKSAFSFK
                     GKKVDPKEIGRQLGVDALLLGSLAVRGADLAITAELVSVRDDTQLWGEKYNRRADDVL
                     RVESEIASTIARTLRRALSGEEKEKLARGRTEDPEAYRLYLKGRDYLVGNQQEMDKSV
                     DYFQQAVARAPDYALAYAGLAEAYTRQAFLRGSGRAEPASKARAAVARALEIDPDQAE
                     AHAALGLVAFYFEWDWAGAETELRRALELNPGSRIVQGHYGWFLTAMGRLDEGLAHCK
                     KAAELDPLSTGPVHDIAINYMVRGDLEQAATMFRRAIDIDPNWTWGYIKLSRTLARQK
                     KCPEALAQAEIAERRIAGGAAPLSRSWLGVTYALCGEPARARQKLDELHALEAKQYVD
                     PVNFSDIHGALGEMDEALRWYEKAFEDRTPNMAYALLGPGISPGLAGNARYEAIVRKM
                     NFPGPAR"
     gene            complement(3341..>5155)
                     /locus_tag="PLB01_16760"
     CDS             complement(3341..>5155)
                     /locus_tag="PLB01_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79006.1"
                     /translation="QAGLPAPVAARRKNREKLAWTLAAAAAAVALVFAIGFVRRAPRV
                     SSPIFAAIPSPEKTFVESIALSPDGKTLAFTAGKAGVQPGLWVRALGSPAAHPVVGAE
                     EASFPFWSPDSRFIGYFTQGSLKKIDPSGGPALTVCDAARGVGGTWNRDGTIVFGPAP
                     TSPLFRVPASGGKPEAVTKLDASLHVTAHRYPVFLPDGRHFLYMAANLSGNTGDPANS
                     IRVGSLDGKTDKAVVAGIASNPSFVAGHLLYGSEGALLAVKMDPARLEVRGDAAPVAQ
                     RLSVTNWYGYTQFTASDDLLLSTPAFAIPSQLAWFDRNGRPAGTVGEPGLWFAPRLSP
                     DGRRIAVGLLDLGRNTSDIWLYDVAGGAGTKFVFGSGNNFSPVWAPAGDRLLFGSDRK
                     AKGSRQDLWTKSLDGSAEEILLESPDARVPEDWSPDGRSLSIDNVQAQGKRNNQLWIV
                     ETTGEKKARPFATEANFQGDSRFSPDGRWLAYASDESGRFEVYVRPFPGPGGRWQIST
                     AGGGQPHWRRDGKELVYLTPENKLMAVPIDSTPGFRAGPPALLFAVHPSLNGAAYDAT
                     GDHQKFLVNTVPDEQGSPPLSLLVHWTRLLEKHEDSRR"
BASE COUNT          768 a         1785 c         1802 g          800 t
ORIGIN      
        1 cgcgggcgcc caccgccggc caccccgggg cggcgccagt gccgccgcgc ggccgctcgc
       61 ggagctcccg gccgacgcct cgaggtcgaa cgcgaggtcg cgggcggact ggaagcgctc
      121 cgccgggttc ttctcgaggc agtgcctcac gagccggtcg agctcgggcg gaatcttcgc
      181 gcccgagacg tccggttccg cgggctcctc cttgaggatc gcgttcatcg tctcgatcgc
      241 cgagtcgccc ttgaacgcgc gccggcccgt cagcatctcg aagaggacgc acccgaacgc
      301 gaaaatgtcc gaccgcgcgt cggccggcag cccccgcact tgctcgggcg ccatgtaccc
      361 gacggtcccg aggagggtcc cggcgtccgt cgccttctcc atcgtgggcg atttcgtgtc
      421 ctctccagcc gtgacggcgc gctgcttcgc gaggccgaag tcgaggatct tcgcgcgccc
      481 gtccttcgtg aggacgacgt tctcgggctt gaggtcccgg tggacgattc cgctctcgtg
      541 cgcggccgcg aggccgcgcg cgatctgggc tccgagcccc acggctttcc ggacgggcag
      601 cgcccccgca gtgatccgct cgcgcagcgt ctcgccgtcc acgagctcca tcacgagcac
      661 ggagacagaa gaagaagaag aactggggat ttcttcgaat gaatagacgg ccgcgatgtt
      721 cgggtggttc agcgccgcga gcagcttcgc ttcccgctcg aatcgctgct ttctctcttc
      781 accttccaag aaatccacgg gcagcagctt cactgcgaca tcgcgattga ggcgagggtc
      841 cctggccttg tagacctccc ccatccctcc cgcaccgatc tggccgagga tctcgtacgg
      901 gccgagcttc gtaccggcaa cgagcgtcat cgcgccggcc ccgggaagtt catcttgcgg
      961 acgatcgcct cgtaacgcgc gttgccggcg agcccggggc tgattccggg tccgaggagc
     1021 gcgtacgcca tgttcggcgt ccggtcctcg aaggccttct cgtaccagcg cagcgcctcg
     1081 tccatctcgc cgagggcgcc gtggatgtcg gagaagttca ccgggtcgac gtactgtttc
     1141 gcctccaggg cgtggagctc gtcgagcttc tggcgggcac gcgcaggctc gccgcacagc
     1201 gcgtacgtga cgcccagcca cgaccgcgac agcggcgcgg cgccgcccgc gatcctcctc
     1261 tcggcgatct cggcctgggc gagggcctcg gggcactttt tctggcgcgc cagggtccgg
     1321 ctcagcttga tgtagcccca ggtccagttc gggtcgatgt cgatcgcgcg gcgaaacatc
     1381 gtcgcggcct gctcgaggtc gccccggacc atgtagttga tcgcgatgtc gtggaccggg
     1441 ccggtcgaca gcgggtcgag ctcggcggcc ttcttgcagt gcgcaagacc ctcgtcgagg
     1501 cggcccatgg cggtcaggaa ccacccgtag tgaccctgaa cgatgcggct gcccgggttg
     1561 agctcgagag cgcgccggag ctcggtctcc gcacccgccc aatcccactc gaagtagaag
     1621 gcgacgagcc cgagcgcggc gtgggcctcg gcctggtccg gatcgatctc gagcgcgcgg
     1681 gcgacggcgg cccgggcctt gctcgccggc tccgcacggc cgctcccgcg gaggaaggcc
     1741 tgtcgggtgt aagcctcggc gagcccggcg taggcgagag cgtagtccgg cgccctggcc
     1801 accgcctgct ggaagtagtc gacgctcttg tccatctcct gctggttgcc cacgaggtag
     1861 tcgcgcccct tcaggtacag gcgataagcc tcggggtcct cggttcgacc gcgggcgagt
     1921 ttctccttct cttccccgct cagcgcgcgg cgcagcgtcc gcgcgatcgt ggacgcgatc
     1981 tcgctctcga cacggaggac gtcgtccgcg cgtcggttgt acttctcgcc ccagagctgg
     2041 gtgtcgtcgc ggacgctcac gagctcggcg gtgatggcga ggtccgcgcc ccggaccgcg
     2101 aggcttccga gcagcagggc gtcgacaccc agctgtcggc cgatctcctt cgggtccacc
     2161 ttcttgccct tgaacgagaa cgcggacttt cgcgagatga cacggagacc gggtagccgc
     2221 gaaagtgtgc tgatcaggct ttcggaaatc ccgtcgctca ggtactcgat cgatgggtcg
     2281 cccgacgcgt tctcgaacgg cagcacggcc accgaaccga tcgaggcgcc gcggccggcc
     2341 cggcggagga cgaggaacgc gcccgcggcg agaagcacgg cgaagccggc cacgccgaga
     2401 aggacgagcc tcttcgacgt gcggaacgga gcggccgacg ccggtctcgt cggcgaggtc
     2461 ggatcgcgct gcagctcgcc gaggtccacg gcgaggtcgt gggccgtggc gtaacgccgg
     2521 tcgcggtcct tctcgaggca acgcccgatc agccgatcga ggcccgccgg cagccccggg
     2581 cgcagctcgc ccggcgggcg cggcgtatcg cgcaggatcg acgagaggag ctcggccgac
     2641 gaatccccgc ggaacggcct ctgcccggtc gccagctcgt agaggatgac tcccagcgag
     2701 aagatgtccg tccggtggtc gagcggccgc ccctgggcct gctcgggcga catgtacggc
     2761 accgtcccca ccacgacgcc ctcctgggtc cgcatgtccg tcgcgagcgc cgagtcgacc
     2821 tgcgccccgg cgccggacgc ggcaaccttc gcgaggccga agtcgagcac cctgacgcgc
     2881 ccacccttcg tcaccatgac gttcgcgggc ttgaggtccc ggtggacgat ccccttctcg
     2941 tgtgccgcct cgagcgcctc ggcgacctga cgggcgagag agatggttac ttcgagggga
     3001 agagggccgg cggcggcgcg ggcatcgaga ccttcgccct cgacgagctc catgacgagg
     3061 acgtggcgcg acattggaga ggaagagaaa gacgaagagg ggatttcttc gaatgagtag
     3121 agggtggcga tccccggatg attcaggctc gcgagcattt tcgcctcgcg ctcgaaccgc
     3181 tgcctcctct cttcaccttc aaagaactct tccggcagga ccttcagggc gaccactctg
     3241 tcgacgcgca cgtccttcgc ccggtagacc tcccccatcc cgccctttcc gaggggcgag
     3301 aggatctcgt aggcgccgag cctcgtgccc gcagtgagcg tcatcggcgg gaatcctcgt
     3361 gcttttcgag gagccgcgtc cagtggacca ggaggctgag ggggggcgag ccctgctcgt
     3421 cggggacggt gttgacgagg aacttctggt ggtctcccgt ggcgtcgtag gccgctccgt
     3481 tcaggctcgg atggaccgcg aacaggagcg cgggcggccc ggcgcgaaat ccgggcgtcg
     3541 agtcgatcgg gaccgccatg agcttgtttt ccggcgtgag atagacgagt tccttcccgt
     3601 cgcggcgcca gtgaggctgg ccgccgcccg ccgtcgagat ctgccagcgg ccgcccggcc
     3661 ccgggaacgg ccggacgtag acctcgaacc gcccggactc gtcggaagcg taggcgagcc
     3721 atcgcccgtc gggagagaac cggctgtcgc cctggaagtt cgcctccgtc gcgaacggac
     3781 gggccttctt ctcccccgtc gtctcgacga tccagagctg gttgttcctc ttcccctgcg
     3841 cctggacgtt gtcgatcgag agagaccgcc cgtccggaga ccagtcctcg gggacccggg
     3901 cgtcgggcga ctcgagcagg atctcttcgg cgctcccgtc gagggacttc gtccagagat
     3961 cctgccgcga ccccttcgcc ttccggtcgg acccgaagag aagccggtcg ccggcgggag
     4021 cccaaacggg cgagaagttg ttcccgctgc cgaacacgaa cttcgtgcca gcccctccgg
     4081 cgacgtcata gagccagatg tcggacgtgt tccttcccag atcgagcagg ccgaccgcga
     4141 tcctccggcc gtcgggcgag aggcgtggcg cgaaccagag accgggctcg ccgaccgtcc
     4201 cggccggccg gccattccgg tcgaaccacg cgagctgcga cgggatggcg aaggccgggg
     4261 tcgagaggag gaggtcgtcc gaggccgtga actgggtgta gccgtaccag ttcgtgacgc
     4321 tcaggcgctg ggcgacgggg gcggcgtctc cccgcacctc gagccgcgcg ggatccatct
     4381 tgacggcgag aagcgctccc tcgctgccat agagaaggtg tcccgcgacg aacgacggat
     4441 tcgaggcgat cccggcgacc acggccttgt ccgttttccc gtcgagcgac cccacccgga
     4501 tcgagttcgc gggatcgccc gtgttcccgg acaggttggc ggccatgtaa aggaagtgcc
     4561 tcccgtccgg aaggaacacg gggtagcgat gcgccgtgac gtgcagggag gcgtcgagtt
     4621 tcgtgaccgc ctccggcttc ccgcccgacg ccgggacgcg gaagagaggc gacgtcggcg
     4681 ccggaccgaa aacgatcgtg ccgtcgcggt tccacgtgcc gccgacgccc cgtgccgcat
     4741 cgcagacggt gagggcgggc ccgccggacg ggtcgatctt cttcagggaa ccctgggtga
     4801 agtaaccgat gaaccggctg tcgggcgacc agaaggggaa gctggcttcc tcggcgccga
     4861 cgaccgggtg cgccgcgggg gagccgagtg ccctgaccca gagaccgggc tgcacgcccg
     4921 ccttccccgc cgtgaacgcc agcgtcttcc cgtcgggaga gagcgcgatc gactcgacga
     4981 acgtcttctc gggagacgga atcgcggcga agatcggcga ggagacgcgc ggggcgcggc
     5041 ggacgaatcc gatcgcgaag acgagcgcga cggccgcggc tgcggccgcg agcgtccacg
     5101 cgagcttctc gcggttcttg cgccgggcgg cgacgggcgc gggaaggccg gcctg
//
LOCUS       DAOMFY010000282         5097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5763_length_5097_cov_5.840738, whole genome shotgun sequence.
ACCESSION   DAOMFY010000282 DAOMFY010000000
VERSION     DAOMFY010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5763_length_5097_cov_5.840738"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3280
                     /locus_tag="PLB01_16765"
     CDS             <1..3280
                     /locus_tag="PLB01_16765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HQQ79007.1"
                     /translation="FWKAAKQAGIKALVGAEVVLGSARFARAGGGARSARARGISSKG
                     EEGHAHHSADALRSGDSPPHPFEEILPGPLGLVPPPLPPGVSLPRLTLLAENQRGYRN
                     LCSLLTAAAMGKPKGQAAAAWEDVAAHAEGLHVLTGGEESAVTRALVSGGPEAAGREL
                     DRLASLFPGRVHVELQRHHLRAEEHQNRALIDLARARRLPLLATNGVRYARREGKDLF
                     DALTCIRHHTHLDAAGTKLHAGRERHLKSAADMQALFSDLPGVLEESAALASRLSFTL
                     QDLGYRFPEYPLPPGETPSSYLRRVTWEGARGRFRPLTTKAQAQLTKELNLIEKLDLA
                     GYFLIVWDIVNFCRREKILVQGRGSAANSAVCYALSITAVDPVRMELLFERFLSEERG
                     EWPDIDLDLPSGDQREKVIQHVYRTYGAHGAAMTANVITYRDRSAAREMAKAVGFSPG
                     QVDTLAKHLGRYSFGEYRDGQRDLSAEMAAAGFDPEEARVKHFGSLFMRVQNLPRHLG
                     QHSGGMVVAMGKLSEVVPLEPAAMEGRVVIQWDKDDCADLGIVKVDLLGLGMLGAIER
                     TIPMIEAHEGERVDLAHIPHDDPAVYAMLQKADTIGLFQVESRAQMASLPRHMPKVFY
                     DIVVQVAIIRPGPIVGGMVHPFFDRRQGKAPVVYPHPCLEPILKRTLGVPLFQEQLLK
                     IAMVAAGFTGGEAEELRRAMGFKRSEERMGAIEARLRSGMEKNGITGPAQDQIVKSIT
                     SFALYGFPESHAASFALIAYASAWLKAHHPAAFTACLLNAWPMGFYHPATIVKDIQRK
                     GVTVLPIDVNSSGWECDLEREEKEREKEEEKISLKVNWTWAAGESRDASPSSPSKKSS
                     SSLGLRLGMRFIKGLKEATGRAIEEERKKRSFEDVSDLQRRCSLRTDEITRLAYAGAL
                     SSLGISRRTALWQVAEVSRPLGTLYEDLPLSSSSQNSPLPEMSAVEETTADYRATDMT
                     AGPHLIEHLRPLLSSRSILSAAELRDARVGDRVKAAGAVIVRQRPGTAKGFVFLSLED
                     ESGIVQAIVRPDLFKENRALIVGSAGLVVEGPLQKEGGNVSVRAERFWPLPALPGVPS
                     HDFR"
     gene            complement(3277..>3504)
                     /locus_tag="PLB01_16770"
     CDS             complement(3277..>3504)
                     /locus_tag="PLB01_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79008.1"
                     /translation="MAAFRDLPGARDFARSSLLIGPSAETGCGTDSLPGLRRALAGDG
                     SPGLEAGRLLVALGAADAKLREAVRLARGGS"
     assembly_gap    3505..3514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3515..>5097)
                     /locus_tag="PLB01_16775"
     CDS             complement(<3515..>5097)
                     /locus_tag="PLB01_16775"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQQ79009.1"
                     /translation="HRAGTPAQASVAETVADALRAVGLEVVVSAFDVDLPEPGEASLA
                     LDGAPAFELAERALPGNPYSADGARETPFFAWAPSGEARGRVVYANHGDRGDYALLAK
                     AGVSVKGAIVLARAGGICRSMKSLLAEEAGAAALLLYPERRDLGVVKPDFPDGPYLNP
                     WAVPRGSMLRYFLYPGDPAGARACATPGLLPRIPALPISETVARELLAKIEGTPAPEA
                     WTGWLRVPYRVGASRSEALLSVRGAKRRATLRNLLATLPGRRREAAAIVVSAHTDAWV
                     HGAIDPVSGAATVLEAADVLAAFSAGGWRPERDVVFAFWDGEEYGMLGSTRWVEERLA
                     DLPRKVAAFLYVDSSARAWDFMADVSPGLAGSLDDVLAAVPDPATGRPLLSVRGTLQL
                     PGFSGDTAPFVGMGGVPGAQVGYGRRTYAMYHTGYDDPLLARRFLDPGWGLSASLARI
                     LSVWSSVLADAPVPAWKFSEISDFLRKEIEGLPAAGSARFPERRDLLAAVARFREAAV
                     AWDDVGIAFRGARAPEVEP"
BASE COUNT          808 a         1806 c         1782 g          691 t
ORIGIN      
        1 cttttggaag gcggcgaaac aggcgggcat caaggcgctc gtcggggcgg aggtagtgct
       61 cggcagcgcg cgcttcgcgc gcgcaggggg aggcgcgcgc tccgcgcgcg cccgggggat
      121 ttcttcgaag ggtgaagagg ggcatgcgca tcactccgcc gatgccttgc gaagcggcga
      181 ttcgccccct cacccattcg aagaaattct tccgggtccc ctcggtcttg tccctccccc
      241 tcttcctcca ggagtttctc tcccccgcct caccctcctc gccgagaacc agaggggtta
      301 ccggaacctc tgctcgctcc tcaccgccgc ggccatggga aagccgaagg ggcaggcggc
      361 ggctgcgtgg gaagacgtcg ccgcgcacgc ggaggggctg cacgtcctga cgggcggaga
      421 ggagagcgcc gtgacgcgcg cgctcgtctc gggcggcccc gaggcggccg gccgggagct
      481 ggaccgcctc gcgtccctct tcccggggcg cgtccacgtc gagctgcagc gccaccacct
      541 gcgcgccgag gagcaccaga accgcgcgct gatcgacctc gcgcgcgcgc ggcgcctccc
      601 gctcctcgcg acgaacggcg tgcgctacgc gcggcgggag ggcaaggacc tcttcgacgc
      661 gctcacgtgc atccgccacc acacgcacct cgacgcggcc gggacgaagc tgcacgccgg
      721 gcgcgagcgg cacctcaagt ccgcagccga catgcaggcg ctcttctcgg acctgccggg
      781 cgtcctcgag gagagcgcgg cgctcgcttc gcgcctgtcg ttcacgctcc aggacctcgg
      841 ctaccgcttc ccggagtacc cgcttccgcc gggcgagacg ccgtcgtctt acctccggcg
      901 cgtgacgtgg gagggcgcgc gcgggcgctt ccgtccgctc acgacgaagg cccaggcgca
      961 gctcacgaag gagctgaacc tcatcgagaa gctcgacctc gcgggctact tcctcatcgt
     1021 ctgggacatc gtgaacttct gcaggcgcga gaagatcctc gttcaggggc gcggctcggc
     1081 cgcgaacagc gccgtctgct acgcgctctc gatcacggcc gtcgaccccg tgcgaatgga
     1141 gctcctcttc gagcgcttcc tctcggagga acgcggcgag tggcccgaca tcgacctcga
     1201 cctgccctcg ggcgaccagc gggagaaggt catccagcac gtctaccgga cgtacggcgc
     1261 acacggggcc gcgatgacgg cgaacgtcat cacgtaccgc gaccgctcgg ccgcgcgcga
     1321 gatggccaag gccgtgggct tttcgccggg ccaggtcgac acgctcgcca agcacctcgg
     1381 gcggtacagc ttcggcgagt accgcgacgg gcagcgggac ctctccgccg agatggccgc
     1441 cgcgggcttc gacccggagg aggcgcgcgt gaagcacttc gggtcgctct tcatgcgcgt
     1501 ccagaacctg ccgcggcacc tcgggcagca ctcgggcggg atggtcgtgg cgatgggaaa
     1561 gctctccgag gtcgtgccgc tcgagcccgc cgcgatggag gggcgtgtcg tcatccagtg
     1621 ggacaaggac gactgcgcgg acctcggcat cgtgaaggtg gacctcctcg gcctcgggat
     1681 gctcggcgcg atcgagagga cgatcccgat gatcgaggcg cacgagggcg agagggtgga
     1741 cctcgcgcac atcccgcacg acgacccggc ggtctacgcg atgctgcaga aggccgacac
     1801 gatcggcctc ttccaggtgg agagccgcgc gcagatggcc tcgctcccgc ggcacatgcc
     1861 caaggtcttc tacgacatcg tcgtccaggt cgcgatcatc cggcccggcc cgatcgtggg
     1921 cgggatggtc cacccgttct tcgaccggcg ccagggcaag gcgcccgtcg tgtacccgca
     1981 cccgtgcctc gagccgatcc tcaagcgcac gctcggcgtg ccgctcttcc aggagcagct
     2041 cctcaagatc gcgatggtcg ccgccggctt cacgggcggc gaggccgagg agcttcggcg
     2101 cgcgatgggg ttcaagcgct ccgaggagcg catgggggcg atcgaggcgc ggctgaggtc
     2161 cggcatggag aagaacggga tcacggggcc cgcgcaggac cagatcgtca agtccatcac
     2221 gtccttcgcg ctctacggct tccccgagtc gcacgccgcg agcttcgcgc tcatcgcgta
     2281 cgcgagcgcg tggctcaagg cccaccaccc ggcggcgttc acggcgtgcc tcctgaacgc
     2341 gtggccgatg ggcttctacc acccggcgac gatcgtgaag gacatccagc gaaagggcgt
     2401 cacggtcctg cccatcgacg tgaactcttc cggatgggaa tgcgatttgg aaagagaaga
     2461 gaaagagaga gagaaagaag aagaaaagat ttctttgaaa gtgaattgga catgggctgc
     2521 cggagagtcg cgcgacgcat cgccctcttc accttctaag aaatcttcct cttctcttgg
     2581 tcttcgtctc gggatgcgtt tcatcaaggg cctcaaggag gcgacgggga gagccatcga
     2641 ggaagagcgg aagaagagat ccttcgaaga tgtttcggac ctgcagcgga gatgctcgct
     2701 gcgcaccgac gaaatcacgc gccttgcgta tgccggagcc ctctcctccc tcggcatttc
     2761 ccgccgcacc gcgctctggc aggtggccga ggtttcccgc ccgctcggga ccctctatga
     2821 agatcttcct ctttcttcct cctcgcagaa ttcccctctc cccgagatgt ccgccgtcga
     2881 ggagacgacg gccgactacc gcgcgaccga catgacggcc gggccgcacc tcatcgagca
     2941 cctgaggccc ctgctctctt ctcgctccat cctctcggcg gccgagctgc gcgacgcgcg
     3001 cgtgggcgac cgcgtgaagg ccgcgggcgc cgtgatcgtg cggcagcggc cgggcaccgc
     3061 gaagggcttc gtgttcctgt cgctcgagga cgagagcggg atcgtgcagg cgatcgtccg
     3121 gcccgacctc ttcaaggaga accgcgcact catcgtcggg tcggccggcc tcgtcgtgga
     3181 ggggccgctg cagaaggagg gcgggaacgt ctccgtccgg gccgagcgct tctggccgct
     3241 gccggcgctg cccggcgtcc cgagccacga cttccgctag gagccccctc gcgcgaggcg
     3301 caccgcctcg cgcagcttcg cgtccgcggc gccgagcgcg acgaggaggc gaccggcctc
     3361 caggcccggc gacccgtcgc cggcgagcgc gcgacgcagg cccgggagcg agtccgtgcc
     3421 gcagcccgtc tcggccgacg ggccgatgag gaggctcgag cgcgcgaagt cgcgtgcgcc
     3481 gggaagatca cggaacgcgg ccatnnnnnn nnnncggctc gacttccggc gcgcgtgcgc
     3541 cgcgaaacgc gatcccgacg tcgtcccagg cgacggcggc ttcacggaaa cgcgccaccg
     3601 ccgcgagaag atcgcgccgc tccggaaacc gcgcggaccc tgcagccggc aggccctcga
     3661 tctcctttct cagaaaatcc gaaatttccg agaacttcca cgcgggcacg ggcgcgtcgg
     3721 cgagaaccga ggaccacacg gagaggatcc gcgcgagcga agccgagagg ccccatccgg
     3781 ggtccaggaa ccggcgggcg agcagcggat cgtcgtagcc cgtgtggtac atcgcgtacg
     3841 tgcgccgccc gtatcccacc tgcgcgcccg gcacgcctcc cattccgacg aacggcgccg
     3901 tgtcgccgct gaagcccggc agctggagcg tcccgcgcac ggagaggagc gggcggcccg
     3961 tggcgggatc cggaaccgcg gcaaggacgt cgtcgagcga gcccgcgagc ccgggcgaga
     4021 cgtccgccat gaagtcccac gcgcgcgcgg aggagtcgac gtagagaaac gccgcgacct
     4081 tccgggggag gtccgcgagg cgctcctcca cccagcgtgt cgagccgagc atcccgtact
     4141 cctcgccgtc ccagaacgcg aagacgacat cccgctccgg ccgccagccg ccggccgaga
     4201 aagccgcgag gacgtccgcg gcctcgagca cggtcgccgc gccggacacg gggtcgatcg
     4261 caccgtggac ccaggcgtcc gtgtgcgcgg agacgacgat cgccgccgcc tcgcggcgcc
     4321 gaccggggag agtcgccagg aggtttctta gagtggcgcg gcgcttcgcg ccgcgaacgg
     4381 aaagcaacgc ttcggatcgg ctcgcgccga cgcggtaagg aacgcggagc cagccggtcc
     4441 aagcttcggg ggcaggcgtc ccctcgatct tcgcaagcaa ctctctggcg acggtctcgg
     4501 aaatcggcag cgccgggatg cgcggcagga ggcccggagt cgcgcaggcg cgcgctcccg
     4561 cagggtctcc cggatagagg aagtaccgca gcatcgagcc gcgcggcacc gcccaggggt
     4621 tcaaataggg gccgtccggg aaatccggct tgacgacgcc gaggtcgcgc cgctccgggt
     4681 agagaaggag cgccgcggcg cccgcctcct cggcgaggag cgacttcatc gagcggcaga
     4741 tcccgccagc gcgcgcgaga acgatcgcgc ccttcacgga aacgcccgcc ttcgcgagaa
     4801 gcgcgtagtc gccccggtcg ccatggttcg cgtagacgac gcgcccgcgc gcctcgccgg
     4861 agggcgccca cgcgaagaac ggggtctcgc gcgcgccgtc ggccgagtag gggttgccgg
     4921 gcagcgcgcg ctcggcgagc tcgaacgcgg gtgcgccgtc gagcgcgagc gacgcttcgc
     4981 ccggctcggg caggtcgacg tcgaacgccg agacgacgac ctccaggccg accgcgcgca
     5041 gcgcgtcggc gacggtctcg gcgacggacg cctgcgccgg ggtgccggcg cggtgcg
//
LOCUS       DAOMFY010000283         5091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5771_length_5091_cov_6.287133, whole genome shotgun sequence.
ACCESSION   DAOMFY010000283 DAOMFY010000000
VERSION     DAOMFY010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5771_length_5091_cov_6.287133"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..904
                     /locus_tag="PLB01_16780"
     CDS             152..904
                     /locus_tag="PLB01_16780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQQ79010.1"
                     /translation="MRRFFIASGLTLSLALPVAAQDAKPAAPKAPAKAAAGAPASEAD
                     KTLYALGLVISRNLAGFKLSAAELKVVEEGMADGVLGRTPKVDIETYGPKLNDLAKSR
                     MAAAAAVEKKAGSAFLEKEAAQPGAKKQASGFVYRETKAGTGATPKATDKVKVHYKGT
                     LIDGTVFDSSIDRNEPVTFPLNGVIPCWTQGVQLMKEGGSARLVCPSELAYGDEGRPP
                     RIKGGATLVFEVQLLSIEKDAPPAAAAPAPKK"
     gene            complement(917..1456)
                     /locus_tag="PLB01_16785"
     CDS             complement(917..1456)
                     /locus_tag="PLB01_16785"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 family protein"
                     /protein_id="HQQ79011.1"
                     /translation="MFGEEEKEFLRRLARAAAERALSMRVSPAASPPALLTFKEIFGL
                     FVTWLKDGRPRGRSGTLAPEGSLSTLIEIHAVEALLHDPRVPPATARELPRYSPYISV
                     LSPFEEISSSSEVVVGTHGVVAEKGKRWGIVLPEDAEGLNWDADRLLGQACLRAGLPE
                     DSWKAAGSVRLRRFTAETF"
     gene            complement(1456..2118)
                     /locus_tag="PLB01_16790"
     CDS             complement(1456..2118)
                     /locus_tag="PLB01_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79012.1"
                     /translation="MLTSPDVTAAPDATLQTVSIPGPAGRLEGLLRSPAVPAGAAVVA
                     HPHPRHGGTMHTKVVQRAAKLLSGRFSLAALRFNFRGVGTSEGAYDDGRGETDDVVAA
                     GKWVRARQPAGPFVVAGFSFGSLCALHASPRLAPDVLFLIGLPIDRWDGAAPAAEPWR
                     VVWIQGEDDEFSPVAKARSVAGARGWTFLSVPGADHFFARKLDEFEKTAGGALAETLA
                     GL"
     gene            2171..3232
                     /locus_tag="PLB01_16795"
     CDS             2171..3232
                     /locus_tag="PLB01_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79013.1"
                     /translation="MKNIVHIVGTGTIGEPLIGLFTDFKQHLGIDEVTFHKRTPMKTE
                     RAKVNHLMKRGALLATEDDKVADFEALGHKISYTAQEALERATVVLDCTPAGNDNKEQ
                     VYKHLKGPAGFLAQGSEFGFGKMYARGINDSALVPGEDKFLQIVSCNTHNLAILIKTL
                     GMDDLGKLTMERAQFVCMRRSNDISQTKDYSPAPTFSKHDDDEFGTHHAHDVFHLYET
                     LNLKPKVFSSALKLNTQYMHSVWFNFELKDKTDKKHVLERLKANPLVALTERKSANEV
                     FSFGRDHGYYGRILSQTVISTPTLSVADEGKEIVGFCFTPQDGNSLLSSVAGAIWLMD
                     PEHASARIQVLKRFVFNEI"
     gene            complement(3337..3795)
                     /locus_tag="PLB01_16800"
     CDS             complement(3337..3795)
                     /locus_tag="PLB01_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ79014.1"
                     /translation="MTAARILVVEDGREYVEGFARLAAAKGSDPAFVHARSLAEAREA
                     LREGRPDAVFLDVVFDRTPEAELAGDLDTLVARYGGDRARAVRHLATHQGFYILDALA
                     DALAGVRVVLAYDFTGEEKRLASLRERVPALSGLPDGASLGTALEDLLRR"
     gene            complement(3792..5069)
                     /locus_tag="PLB01_16805"
     CDS             complement(3792..5069)
                     /locus_tag="PLB01_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79015.1"
                     /translation="MDPSRASSWERLAALLEPLPGRGADATAAWQKAEQAGSREALWA
                     LGRRAASGGRELEAVRWWRRYVAEAPDGLHLDEARAAVERMDRRFQTLRGGGIGLAAF
                     GVLLGAGLLLRRRRGLTLAEWLRRDPGATREARPVLGRLSHEVFKHGGLLLGGAEESL
                     GSGEDFRPSAEILTARLYGSGAGPGLVAEGRAALDDLAAVSRRRGAALNLRHKDPVLA
                     PVAAALDTLDAAHDDLASLAAGRTLPARRRASLAARLGQAAETLNARTAQDLNRLLDA
                     AGSVQASWDGLAALLASVARERKAPAPVLEPMGLFAEAGAPLRVKLPGAEWETIFRNL
                     FANALDSPRLGLLAEQRRDPVTGQAQGRFVLYDADPRPLTAEMIRGRAAERGLGVVAD
                     AVRRWDGAVDVVPGATGYAKGVSVEFAVVEEPS"
BASE COUNT          845 a         1823 c         1742 g          681 t
ORIGIN      
        1 gctcacgctt ccgctcggcg gaaacgtcga gtccctgaac gcggccgtcg cggccgggct
       61 gctgcttttc gttctcagcc ggccctgacg gggttaccat cccggatcgg ccgcccagcc
      121 ggccgcccac cacagaaagc gaggactcaa catgcggcga ttcttcatcg cctccggtct
      181 caccctgtcc ctggcgctcc cggtcgccgc ccaggacgcc aagcctgcgg ccccgaaagc
      241 gccggccaag gccgccgcgg gtgcgcccgc cagcgaagcc gacaagacgc tctacgcgct
      301 cgggctcgtc atctcgcgca acctcgcggg cttcaagctc tccgccgcgg agctcaaggt
      361 cgtggaggag ggcatggccg acggcgtcct cggcaggacg ccgaaggtcg acatcgagac
      421 gtacggccca aagctgaacg acctcgccaa gagccgcatg gcggcggcgg ccgcggtcga
      481 gaagaaggcc ggctccgcgt tcctcgagaa ggaagccgcc cagcccggcg cgaagaagca
      541 ggcgtccggt ttcgtctacc gggagacgaa ggcgggcacg ggcgcgacgc cgaaggccac
      601 cgacaaggtc aaggtccact acaagggcac gctgatcgac ggcaccgtct tcgacagctc
      661 gatcgaccgc aacgagcccg tcaccttccc gctcaacggc gtcattccct gctggacgca
      721 gggcgtgcag ctcatgaagg aaggcggctc ggcgcgtctc gtgtgcccgt cggagctcgc
      781 ctacggcgac gagggccgcc cgccgcgcat caagggcggc gccacgctcg tgttcgaggt
      841 ccagctcctc tcgatcgaga aggacgcgcc gcccgccgcg gccgctcccg ccccgaagaa
      901 gtgatcccgg agggcgtcag aacgtctcgg ccgtgaaccg gcggaggcgg accgaccccg
      961 ccgccttcca gctgtcctcc ggcaggcccg ccctcaggca ggcctgcccg aggaggcggt
     1021 cggcgtccca gttcaaccct tccgcgtcct cgggaaggac gatcccccag cgctttccct
     1081 tctcggccac gactccgtgt gtcccgacga cgacctcgga ggaagaagag atttcttcga
     1141 agggggagag aacggagatg tacggcgagt agcgcgggag ctcgcgagcg gtcgccgggg
     1201 ggacgcgcgg gtcgtggaga agcgcctcca ccgcgtgaat ttctatgagt gtggagaggg
     1261 acccctcggg ggcgagcgtc ccgcttctgc ctcgaggccg gccgtctttc agccaggtga
     1321 cgaagagacc gaagatttct ttgaaggtaa gaagggcggg cggcgaggcc gcgggcgaga
     1381 cacgcatgct caaggcgcgc tcggcggcgg ccctcgcaag ccttctgaga aactctttct
     1441 cttcctctcc gaacatcaca gccccgcgag cgtttccgcg agagccccgc ccgcggtctt
     1501 ctcgaactcg tcgagcttcc tcgcgaagaa atggtccgcg ccgggcacgg agagaaacgt
     1561 ccagccgcgt gccccggcga cgctccgggc cttggcgacc gggctgaact cgtcgtcctc
     1621 gccctggatc cacacgacgc gccagggctc cgccgccggc gccgcgccgt cccagcggtc
     1681 gatcgggagt ccgatcagaa agaggacgtc gggcgcgagt cggggagacg cgtggagcgc
     1741 gcacagggac ccgaaggaaa agcccgcgac gacgaacggc ccggccggct ggcgagcgcg
     1801 cacccatttc cccgcggcga cgacgtcgtc cgtctccccg cggccgtcgt cgtacgcccc
     1861 ctcggacgtc ccgacgcccc ggaagttgaa gcgcagggcg gcgagcgaga accggccgga
     1921 gagcagcttc gcagcgcgct ggacgacctt cgtgtgcatg gtcccgccgt gccgggggtg
     1981 gggatgggcg accaccgcgg cgccggcggg gacggccgga gaccggagca gcccctccag
     2041 ccggcccgcg ggccccggga tcgacacggt ctggagcgtc gcgtccgggg cagctgtcac
     2101 gtccggcgac gttagcacgg ggctcctata aacttcgcgt ccccaggacc atcgcgtcga
     2161 ggagcggtac atgaagaaca tcgtccacat cgtcggcacg ggcacgatcg gcgagcccct
     2221 catcggactc ttcaccgact tcaagcagca tctcgggatc gacgaagtga cgtttcacaa
     2281 gcgcacgccc atgaagacgg agcgcgcgaa ggtgaaccac ctcatgaagc gcggcgcgct
     2341 cctggccacg gaagacgaca aggtcgccga tttcgaggcg ctcggccaca agatctcgta
     2401 cacggcgcag gaggcgctcg agcgtgcgac cgtcgtcctc gactgcacgc cggccggtaa
     2461 cgacaacaag gagcaggtct acaagcacct gaagggcccc gcgggcttcc tcgcccaggg
     2521 ctcggaattc gggttcggga agatgtacgc gcgcgggatc aacgactcgg cgctcgtccc
     2581 cggcgaggac aagttcctcc agatcgtctc gtgcaacacg cataacctcg cgatcctgat
     2641 caagacgctc gggatggacg acctcggcaa gctcacgatg gagcgcgccc agttcgtctg
     2701 catgcgccgg tccaacgaca tttcccagac gaaggactac tcccccgctc cgacgttctc
     2761 caagcacgac gacgacgagt tcgggacgca ccacgcgcac gacgtcttcc acctgtacga
     2821 gaccctgaac ctcaagccga aagtcttctc gtcggctctc aagctcaaca cgcagtacat
     2881 gcactcggtc tggttcaact tcgagctgaa ggacaagacc gacaagaagc acgtcctcga
     2941 gcgcctcaag gcgaacccgc tcgtcgcgct cacggagagg aagagcgcga acgaggtctt
     3001 ctcgttcggc cgcgaccacg ggtactacgg ccgcatcctg tcccagacgg tcatctcgac
     3061 gccgacgctc tcggtcgccg acgagggcaa ggagatcgtg ggcttctgct tcacgccgca
     3121 ggacggcaat tcgctcctct cgtcggtggc cggagcgatc tggctcatgg accccgagca
     3181 cgccagcgct cgcatccagg ttctcaagcg gttcgtgttc aacgagattt agagagacga
     3241 cgagaggaag aaaagaaaga gaagattttc ttagtaaggc gaagagggca ttggccgcgc
     3301 cgcggctcgc gagggtcgcc ggcgcccggg ctgcgtttac cttctaagaa gatcttctag
     3361 agcggttccg agggacgccc catccggcag gccactgagc gcgggtacgc gttcccggag
     3421 cgaagccaat ctcttctctt ctcccgtgaa gtcgtaagcg aggacgacgc ggacgccggc
     3481 gagcgcgtcc gcgagcgcgt cgagaatgta gaagccctgg tgcgtcgcga ggtggcggac
     3541 ggcgcgcgcg cggtcgccgc cgtaacgcgc gacgagggtg tcgagatcgc ccgcgagctc
     3601 ggcctccggc gtccggtcga agacgacgtc gagaaagacc gcatcgggtc tcccctcccg
     3661 gagcgcctcg cgagcctcgg cgaggctgcg tgcgtgcacg aacgccggat ccgagccctt
     3721 cgcggccgcg aggcgcgcga agccctccac gtactcgcgt ccgtcctcga cgacgaggat
     3781 ccgcgcggcc gtcacgaggg ctcctcgacg acggcgaact cgacggagac gcccttcgcg
     3841 tagccggtgg cgccgggcac gacgtccacc gcgccgtccc agcgcctcac ggcgtcggcc
     3901 acgaccccga ggccgcgttc ggcggcgcgc ccgcggatca tttcggcggt cagaggacgc
     3961 ggatccgcgt cgtagagaac gaagcggccc tgcgcctgcc ccgtgacggg atcgcgcctc
     4021 tgctcggcga ggaggccgag gcgcggcgag tcgagagcgt tcgcgaagag gttccggaag
     4081 atcgtctccc actctgcgcc cggcagcttc acgcgcagcg gagcgcccgc ctccgcgaag
     4141 aggcccatcg gctcgagcac cggggccggc gccttcctct cgcgcgccac ggaggcgagg
     4201 agggccgcaa ggccgtccca cgaggcctgt acggacccgg ccgcgtcgag caaccggttc
     4261 aggtcctggg ccgtgcgcgc attcagcgtc tcggccgcct ggccgaggcg cgccgcgagc
     4321 gacgctcttc gccgtgcggg cagcgtccgg cccgccgcga gcgacgcgag gtcgtcgtgc
     4381 gcggcgtcga gcgtgtcgag cgctgccgcg acgggagcaa ggaccgggtc cttgtgcctg
     4441 aggttcaggg cggcgccccg gcggcgcgag acggccgcga ggtcgtcgag ggccgcgcgc
     4501 ccttcggcca cgaggccggg ccccgcgccc gagccgtaga gacgggccgt gaggatctcg
     4561 gcgctggggc ggaagtcctc gcccgacccc agactctcct cggcgccgcc gaggagaagg
     4621 ccgccgtgct tgaagacctc gtgcgacagc cggcccagga ccgggcgcgc ctcgcgcgtg
     4681 gctccgggat cgcggcgcag ccattcggcc agcgtgaggc cgcggcgccg tctcaggagg
     4741 agcccggccc cgaggaggac gccgaaagcc gcgaggccga tccctccgcc tctaagcgtc
     4801 tggaaccgcc ggtccatccg ctccaccgcg gcacgcgcct cgtcgaggtg cagaccgtcc
     4861 ggcgcctcgg ctacgtagcg ccgccaccag cggaccgcct cgagctctct cccgccggac
     4921 gcggcccggc ggcccagggc ccacagcgcc tcgcgcgagc ccgcctgctc ggctttctgc
     4981 cacgcggccg tcgcgtcggc tccccggccc ggcagcggtt cgaggagggc cgccagccgc
     5041 tcccacgacg aggcgcggga cgggtccacc tacagagcct tcgtcagcgc c
//
LOCUS       DAOMFY010000284         5051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5854_length_5051_cov_8.247198, whole genome shotgun sequence.
ACCESSION   DAOMFY010000284 DAOMFY010000000
VERSION     DAOMFY010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5854_length_5051_cov_8.247198"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLB01_16810"
     CDS             complement(<1..999)
                     /locus_tag="PLB01_16810"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HQQ79016.1"
                     /translation="MREGAERISPLAPPPAARMDWESFVGIKLFSWIAGIFLTIGAIL
                     FLRYSIDRGWLTEPVQMAIGLAAGAGLLVVCELRAAQRYAVTANALDAAGLVILFSTL
                     FAAHARWGILGSGATFASLAAVAALAVLLAVRHDSTFIALLGLLGGFATPVLLGTTWR
                     VLFPFLGLLAVGLFVFAVLRRAPLLHTLSGLATFVVLAGWFVRSLTPDLLAAALTAAC
                     GLSFFFLFAPALGARLAPPDPLPASAAGLGLMGHALLLYVAAEKSLSAPPAVSEALAL
                     FALTAAATFAARRPGAAWVHAAGLSLALLAVTSFTFTARVAPWPLAALGAAGAVALW"
     gene            complement(1369..2622)
                     /locus_tag="PLB01_16815"
     CDS             complement(1369..2622)
                     /locus_tag="PLB01_16815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ79017.1"
                     /translation="MDLAARLKVLRTGFTRTFWVANVLELFERFAFYGSKAVLAVYLN
                     RAVGLGTTGNDLVGVYGLLVFGLPIFAGVVVDRFGFKRSLLACFSIFCAGYTLIGLAG
                     LPAGQPFVDALGKKGYVVLALVVTAIGGSLIKPCIVGTVARTTTEETKSLGYSIYYSL
                     VNFGGFFGPILAGEVRTRFGIASVLLMSAGVSAALVLGTLAFYREPEGPREGRTFAKV
                     ISDASLVLRNVRFIGFLVIFSGFWIMFWQVFYALPFYVTDILKIERFELLETVDALGI
                     IFLSVPATALMKKVRPIRAMAGGFAVASASWLVITLTQTWQGVVAAMLLFALGESMQA
                     PRFYEYVADLAPEGQTGTYMGFAFLPVAIGAFVAGRLSGYLIEHYLKGPNPGGMWILL
                     SAIGFGSTAAMLLYDRFVAPKKAVA"
     gene            complement(2651..2911)
                     /locus_tag="PLB01_16820"
     CDS             complement(2651..2911)
                     /locus_tag="PLB01_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ79018.1"
                     /translation="MGMYIIAEPCIGVKDTACVEVCPVDCIHPKKDEAGHAEATQLYI
                     DPNTCIQCGNCEPVCPPKAIFTEETIPEKWKHYQQINADHYA"
     gene            3008..4423
                     /locus_tag="PLB01_16825"
     CDS             3008..4423
                     /locus_tag="PLB01_16825"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ79019.1"
                     /translation="MHARRFAFVMLLAAPLSAAPPPAPPALAPHEADAREILRRAVAY
                     RTSIGLGQVPALAEYLASRFKAAGFPESDIHVIPYGESASLVVRYRGEPRPAAARKKP
                     IAFLGHMDVVTAKPEDWKRDPFTLVEENGYFFGRGTCDDKNTIALLTATFLRLKAEGF
                     VPARDLVLVLTGDEETEGAHARDLVTNRRDLVDAEFALNGDAGGGTLAEEDGRPVSYG
                     LQTAEKTYASFELTVRNAGGHSSLPRKDNAIYELADALKAVQATRFPVMWNDTTRAWF
                     RVTGARTAGPLGDAMRRFAADPKDAGAAAVLGDSPAEVGATRTTCIPTLLRGGHADNA
                     LPQSASATVNCRIFPGVKVDDVRALLQKATGAGVTVATLGRPEPSDESPLRPDVLAAV
                     EKAVHARRPGVPVAPYQESGGTDGRLFRAAGIPTYGVGETFIKDSDQFAHGLNERIPV
                     ASFYDGLEHWAVLVKELAGPR"
     gene            complement(4436..4699)
                     /locus_tag="PLB01_16830"
     CDS             complement(4436..4699)
                     /locus_tag="PLB01_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79020.1"
                     /translation="MARSRMDDILGTVRDAGDRSQEIASQVAARVEDVLDEAGAQGKR
                     VRKELARRWKHVDRMGRDNAFVMAFGALAVGVLIGYLVARDDD"
     gene            4765..>5051
                     /locus_tag="PLB01_16835"
     CDS             4765..>5051
                     /locus_tag="PLB01_16835"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HQQ79021.1"
                     /translation="MDDREILTRLYRAAVAAVDPAALVSEALGRRGDAVVVRSVSSTA
                     RREFVFAPDRVTVLAVGKAAVPMTAAAAGCLGRRLDGALVVNASGLAAPDLA"
BASE COUNT          839 a         1761 c         1815 g          636 t
ORIGIN      
        1 ccacagggcg acggcgcccg ccgccccgag ggcggcgagc ggccagggcg ccacgcgcgc
       61 cgtgaacgtg aaggacgtga cggcgaggag cgcgagggag agacccgccg cgtgcaccca
      121 ggcggccccg gggcggcgcg ccgcgaaggt ggccgccgcc gtcagcgcga agagggcgag
      181 ggcctcggaa accgccggcg gcgcgctgag gcttttctcg gccgcgacgt agagaaggag
      241 cgcgtgaccc atcaggccga ggcccgcggc actggccggg agcggatccg gcggcgcgag
      301 acgcgcgccg agcgccggcg cgaacaggaa gaagaacgag agaccgcatg cggccgtgag
      361 ggccgcagcg aggaggtccg gcgtcagcga acgcacgaac caccccgcga gcaccacgaa
      421 cgtcgcgagc cccgagagcg tgtggaggag cggcgccctc cggaggacgg cgaagacgaa
      481 gaggcccacg gcgagaaggc ccaggaacgg gaagaggacg cgccaggtcg tcccgagcag
      541 gaccggcgtc gcgaagccgc cgaggagacc gaggagcgcg atgaacgtcg aatcgtggcg
      601 gaccgcgagg agcacggcga gggccgcgac ggcggcgagc gaggcgaacg tcgcgccgga
      661 gccgaggatg ccccagcgcg cgtgggccgc gaagagcgtc gagaacagga tgacgagacc
      721 cgcggcgtcg agcgcattcg cggtcacggc gtacctctgc gccgcgcgca gctcgcacac
      781 gacgaggagg cccgctccgg ccgcgagacc gatcgccatc tgcacgggct cggtgagcca
      841 gccgcggtcg atcgagtagc gcaggaagag gatcgcgccg atggtcagga agatcccggc
      901 gatccacgag aagagcttga tcccgacgaa gctctcccag tccatgcgcg ccgccggagg
      961 cggcgcgagg ggagaaattc tctctgcccc ctcgcgcatc ggcaagccag cctcgaaggc
     1021 acgaggcggc gcagccgcat cggtggcttg cttagaaggt gtaatgggcg gtggagcaaa
     1081 ggccaccggc ggcgccggga atgcgactgg cggtatggag gtatccgtcg cgcggggcgg
     1141 cgccgcgctt tgctccgcag ccccattcac cttcgaagag aaatccaccg gcgcggcctg
     1201 cgttgccgaa acggacgacg aggcaggcga ggaggtccga gagggagcag aaagtctcct
     1261 gacctcgttt tccagccgca cgacgcgctc gcgcagccag gccgcgaggc cgagggcggc
     1321 gaaggcgacg agcagcgtga agaacgtgaa catgccggcg ctccgccgtc aggcgacggc
     1381 cttcttcggc gcgacgaacc ggtcgtacag gagcatcgcg gcggtcgaac cgaatccgat
     1441 cgccgaaagc aggatccaca tcccgccggg gttcgggccc ttgagatagt gctcgatgag
     1501 gtaccccgac aggcgccccg ccacgaacgc gccaatcgcg acgggcagga acgcgaagcc
     1561 catgtacgtg cccgtctggc cttcgggcgc gaggtccgcg acgtactcgt agaagcgcgg
     1621 cgcctgcatg gactcgccga gcgcgaagag gagcatcgcg gcgacgacgc cctgccaggt
     1681 ctgggtgagc gtgatcacga gccacgacgc gctcgcgacc gcgaagccgc ccgccatcgc
     1741 gcggatcggc cgcaccttct tcatgagcgc cgtcgcgggg accgagagga agatgatccc
     1801 gagggcgtcg acggtctcga ggagctcgaa ccgctcgatc ttcaggatgt ccgtcacgta
     1861 gaacggcagc gcgtagaaga cctgccagaa catgatccag aagcccgaga agatcacgag
     1921 gaacccgatg aagcggacgt tccggaggac gagcgaagcg tcggagatga ccttcgcgaa
     1981 cgtccggcct tcgcgcggcc cctcgggctc gcggtagaac gcgagcgtcc cgagcacgag
     2041 cgcggccgag acgccggccg acatcaggag gaccgacgcg atgccgaacc gcgtccggac
     2101 ttcgcccgcg aggatcggcc cgaagaaccc gccgaagttc acgagcgagt agtagatcga
     2161 gtagccgagg gacttcgtct cctcggtcgt cgtccgcgcg acggtcccga cgatgcaggg
     2221 cttgatgagc gagccgccga tcgccgtgac gacgagcgcg aggacgacgt agcccttctt
     2281 cccgagcgcg tcgacgaacg gctgcccggc cggaaggccc gcgagcccga tgagggtgta
     2341 cccggcgcag aagatcgaga agcaggcgag gaggctccgc ttgaagccga agcggtccac
     2401 gacgacgccc gcgaagatcg ggaggccgaa cacgaggagg ccgtagaccc cgacgaggtc
     2461 gttgcccgtc gtgccgagcc cgacggcccg gttcaggtag acggcgagga ccgccttgct
     2521 gccatagaac gcgaagcgct cgaacagctc gaggacgttc gcgacccaga acgtgcgcgt
     2581 gaaccccgtg cggagaacct tgagtcgtgc ggcgagatcc aagagtcctc cggcgcgcgt
     2641 gcgtcgacgg tcaggcgtag tggtcggcgt tgatctgctg gtagtgcttc cacttctcgg
     2701 ggatcgtctc ctccgtgaag atggccttcg gcgggcacac gggctcgcag tttccgcact
     2761 ggatgcacgt gttcgggtcg atgtagagct gcgtcgcctc ggcgtgcccg gcctcgtcct
     2821 tcttcgggtg gatgcagtcc acggggcaga cctcgacgca cgcggtgtcc ttcacgccga
     2881 tgcagggctc cgcgatgatg tacatgccca tggtccgccc tccgcggagg gagattagtc
     2941 aatccccggg gaccggtcca gcgcgacggc ggcgcccgcg ccgctaagat ggcgagagga
     3001 gtctttcgtg cacgctcgtc gcttcgcgtt cgtcatgctg ctcgcggccc cgctctcggc
     3061 cgctccgcct cccgcgcctc ccgccctcgc gccccacgag gccgacgcgc gggagatcct
     3121 ccgccgggcc gtggcgtacc ggacgtcgat cgggctcggc caggtgcccg cgctcgcgga
     3181 gtacctcgcg tcgcggttca aggccgcggg attcccggag tccgacatcc acgtgatccc
     3241 ctacggcgaa tccgcctcgc tcgtcgtccg ctaccggggc gagccccgcc cggccgcggc
     3301 ccggaagaag ccgatcgcct tcctcgggca catggacgtc gtgaccgcga agcccgagga
     3361 ctggaagcgc gacccgttca cgctcgtcga ggagaacggg tacttcttcg ggcgcggcac
     3421 gtgcgacgac aagaacacca tcgccctcct cacggcgacg tttctccgtc tgaaggcgga
     3481 agggttcgtt ccggcccgtg acctcgtgct cgtgctgacg ggcgacgagg aaaccgaggg
     3541 cgcgcacgcg cgcgacctcg tcacgaaccg ccgcgacctc gtcgacgccg agttcgccct
     3601 gaacggcgac gcggggggcg gcacgctggc ggaagaggac gggcgccccg tttcgtacgg
     3661 cctccagacg gcggagaaaa cgtacgccag cttcgagctg accgtgcgca acgccggcgg
     3721 ccacagctcg ctgccgcgca aggacaacgc gatctacgag ctcgccgacg cgctgaaggc
     3781 cgtgcaggcc acgcgcttcc ccgtgatgtg gaacgacacg acgcgcgcct ggttccgggt
     3841 caccggggcg cgcaccgcgg gcccgctcgg agacgcgatg cgccgcttcg ccgccgaccc
     3901 gaaggacgcc ggggccgccg ccgtcctcgg ggacagccct gccgaagtcg gggcgacgcg
     3961 gaccacctgc atcccgacgc tcctccgcgg cggccacgcc gacaacgcgc tgccgcagtc
     4021 cgccagcgcc accgtcaact gccgcatctt tcccggcgtg aaggtggacg acgtgagggc
     4081 cttgctgcag aaggccacgg gcgcgggcgt gacggtcgcg acgctcggca ggccggagcc
     4141 cagcgacgag tcgcctctcc gccccgacgt cctggccgcg gtcgagaagg cggtgcatgc
     4201 gcggcgcccc ggggtccccg tcgcgccgta ccaggagtcg ggcggcacgg acggacggct
     4261 ctttcgcgcg gccggcatcc cgacctatgg tgtcggcgag acgttcatca aggacagcga
     4321 ccagttcgcc cacgggctga acgagcggat ccccgtcgca tccttctacg acggcctcga
     4381 acactgggcc gtcctcgtga aggagctggc cggcccgcgt tgaagaccgg acgcgtcagt
     4441 cgtcgtcgcg ggcgacgagg tagccgatca ggaccccgac ggcgagcgcg ccgaacgcca
     4501 tcacgaacgc attgtcgcgg cccatgcgat ccacgtgctt ccagcggcgc gcgagctcct
     4561 tgcggacgcg cttgccctgg gcgccggcct cgtcgagcac gtcctcgacg cgcgcggcga
     4621 cctgcgaagc gatctcctgg ctgcggtcgc ccgcgtcgcg gaccgttccg agaatgtcgt
     4681 ccatccgtga gcgtgccatg gcagttctcc tgcatgcgag tttacccctc ccgcccgcgc
     4741 cagtttcgct agactccggg cgctttggac gaccgcgaga tcctgacgcg gctctaccgc
     4801 gccgccgtcg ccgccgtgga cccggccgcg ctcgtctccg aggctctcgg gcggcggggc
     4861 gacgcggtcg tcgttcgttc cgtgtcgtcg accgcgcgcc gcgagttcgt cttcgcaccc
     4921 gaccgcgtca ccgttctcgc cgtcgggaag gccgccgtgc ccatgacggc cgccgccgcg
     4981 ggttgcctcg gccgccggct cgacggggcg ctcgtcgtca acgcgagcgg actcgctgca
     5041 cccgatctgg c
//
LOCUS       DAOMFY010000285         5051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5855_length_5051_cov_7.058447, whole genome shotgun sequence.
ACCESSION   DAOMFY010000285 DAOMFY010000000
VERSION     DAOMFY010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5855_length_5051_cov_7.058447"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2582)
                     /locus_tag="PLB01_16840"
     CDS             complement(<1..2582)
                     /locus_tag="PLB01_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQQ79022.1"
                     /translation="MTRRPLRSLAAAVLLLASGAARAAAPSETLSASEIALQLKKLNV
                     VGSVLYVAAHPDDENTAFLAWGAKGRLLETGYLSVTRGDGGQNLIGNETGELVGVLRT
                     QELLAARRIDGARQFFTRAIDFGYSKNPEETFAIWGHEQVLSDVVWIIRSFRPDVIVT
                     RFPTTGEGGHGNHTASAILAGEAFAAAADPTRFPEQLRWVRPWQARRIFWNVFRFGSD
                     APRNAAPGQIALDLGGYNALLGRSYTEIAGQSRSMHKSQGFGAAERRGTWMNDFKLTA
                     GDPATSDLFEGVDLSWKRYGERGEEVGKILRKVEGDFRETDPAASVPGLVQAWTALGR
                     MIDAASEPLNSPSKKIPGAGSNEGDRRGEGPKQNDPLLAAKRAEVVEAIRACLGLWTE
                     AIAADVSAAPGAEVKVATMILNRSSVPAALEKVEVTHAAAPLPGGALAANEPVRGSAT
                     VALPADAPVTQPYWLRAPAGKGLYAVPDATLVGRPENAPPLVARFAVKVAGASIPFET
                     PVVFRRTDPVKGEVYRPFEIVPPVTANFDDKVYAFGSGAAKTVRVTLVAGAPNVAGVL
                     RLKAPPGFRVSPAEAPFTLGARNEEKAVAFTVTPPAAHSSGTLAAETTVAGKTGSRAL
                     VRVDYPHIPMQTLFPPAEARVLRIDVKAPHLPVGYVMGSGDAGPDALKQMGYAVTLLS
                     DDELETGDLSRYSAVVAGIRAFNTRPRLKQAVKRLEAYAEGGGTVVVQYNTTGDLVTD
                     ELGPYPIKLSRDRVTVEEAPVTFVKPDSPLLTFPNKLTPADFDGWVQERGLYFAGSWD
                     PRYETVLATHDPGESDKPGGLLYAKVGKGAWVYTGYAFFRQLPAGVPGAYRLFVNLVS
                     AGAGA"
     gene            complement(2607..3914)
                     /locus_tag="PLB01_16845"
     CDS             complement(2607..3914)
                     /locus_tag="PLB01_16845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ79023.1"
                     /translation="MEPLRPRRIAVSSAFRSRRLLVWGVLALVYVVSYFHRIAPAVLA
                     KDLMRSFAATGVEVATMAALYLYAFAAMQFVVGAAVDAWGPRKAVAAGALVMGVGGAV
                     FAAAHALPLAYGARVVVGVGASVVFIGTLKQIGAWFRPDEFGTWSGLTQVAGNLGGLM
                     AAAPLALVVSLAGWRTTFGGVAALSVALALLTLAVVRDRPEDAGFPPPLPRGPVPKLS
                     VVQGYRSVWGNRRTWPMFLVFFCQYGTLISFSGLWAVPWMRDVYGLSAKEAAQLVSLV
                     GVGVVFGAPAAGFLSDRVFRARRLPYVLFTFVYTAVWACVAVPTGGPPRALLGPLCFL
                     VGLTASCFTLTWVLAREVNPPRFSGIATATVNAGGFLGAAVMQNVLGRILDSHWLGVI
                     EQGARRYPTAGYRAAFLASLGALLVACAGTFFVRETFGKHAAD"
     gene            complement(3902..>5051)
                     /locus_tag="PLB01_16850"
     CDS             complement(3902..>5051)
                     /locus_tag="PLB01_16850"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ79024.1"
                     /translation="AWLPAIRGSGGFAPFREQLLIQAADLRRSSALSARDLLSLSLWR
                     RWLVDPFGSDTRGAVVGAAAAAGALLRRRTASRILLLVLPWTLVNVPVSTLYGAPRYG
                     ALLLGGVAGLAACGLAALASRVPRPGACASVALVAACAWVAVPPVAAASAPSPSASAV
                     RAASQPPYDRGTLVFDPELRMHVSRFLPERAQSEIAPDRAVAAAEGDVVLTADRRVEG
                     LASERVFGRPDPLLARISLGFLLETRIGIAPRRLSVGRRPGGREDDVIRYDASIPVAV
                     DAPAEGAALGGTLDVRGWCQLRGGGAVEPVEFRVDGVLARVLALERTPRPDVAAAVPE
                     IGDASRAGYTARLDASGLAGGAHDLKVTFRAADGRRRIAQPVRFTWNP"
BASE COUNT          769 a         1753 c         1907 g          622 t
ORIGIN      
        1 gcgcccgcgc ctgccgaaac caggttgacg aagagccggt aagcgccggg cacgcccgcc
       61 ggcagctggc ggaagaacgc gtatcccgtg tacacccacg cgcccttccc gaccttcgcg
      121 tagaggaggc cgccgggctt gtcgctctcg ccgggatcgt gcgtcgcgag gaccgtctcg
      181 tacctcggat cccacgaccc cgcgaagtag agcccgcgct cctgcaccca gccgtcgaag
      241 tcggcgggtg tgagtttgtt cgggaaggtc aggaggggac tgtccggttt gacgaacgtc
      301 acgggcgctt cctcgaccgt cacgcggtcg cgcgagagct tgatcgggta cggcccgagc
      361 tcgtccgtga cgaggtcgcc ggtcgtgttg tactgaacga cgaccgtgcc gccgccttcc
      421 gcgtacgcct cgagccgctt gacggcctgc ttgaggcgcg ggcgcgtgtt gaacgcccgg
      481 atccccgcga cgacggccga gtagcgcgag aggtcgcccg tctcgagctc gtcgtccgag
      541 agaagggtca cggcatagcc catctgcttg agcgcgtccg gcccggcgtc gcccgagccc
      601 atgacgtagc cgaccggaag gtgcggcgcc ttgacgtcga tcctcaggac gcgcgcctcc
      661 gcgggcggga agagcgtctg catggggatg tgcgggtagt ccacgcggac gagtgcgcgc
      721 gagccggtct tcccggcgac cgtcgtctcg gccgcgagcg tgccggatga atgggcggcc
      781 ggcggcgtca cggtgaaggc gacggccttc tcctcgttcc gtgcgccgag cgtgaacggc
      841 gcctcggcgg gggaaacgcg gaacccgggg ggcgccttga ggcgcagcac gccggcgacg
      901 ttcggcgcgc cggcgacgag cgttacgcgc acggtcttcg cggcgccgct cccgaacgcg
      961 tagaccttgt cgtcgaagtt cgcggtgacg ggcgggacga tctcgaacgg gcgatagacc
     1021 tcgcccttga ccgggtcggt gcgccggaag acgacgggcg tctcgaaggg gatcgacgct
     1081 ccggccacct tgacggcaaa ccgcgcgacg aggggcggcg cgttctcggg gcggccgacg
     1141 agtgtcgcgt ccggcaccgc gtagagcccc ttgccggcgg gcgcgcgcag ccagtacggc
     1201 tgtgtcaccg gagcatccgc ggggagagcg accgtggcgc tgccgcgcac gggctcgttc
     1261 gcggcgagag cgccgcccgg aagcggcgcg gccgcgtggg tgacctcgac cttctcgagc
     1321 gccgcgggga cgctcgagcg gttcaggatc atcgtcgcga ccttgacctc cgcgcccggc
     1381 gcggccgaga cgtcggccgc gatcgcctcg gtccacagcc cgagacatgc ccggatcgcc
     1441 tcgacgacct cggcgcgctt cgcggcgaga agaggatcgt tttgcttcgg accctctccg
     1501 cgcctgtcgc cttcgttcga accagcaccg gggattttct tagaaggtga attgagcggc
     1561 tcgcttgccg cgtcgatcat ccgcccgagg gcggtccagg cctggaccag cccggggacg
     1621 gaggccgccg ggtccgtttc gcggaagtcc ccctccacct ttctaagaat ctttccgacc
     1681 tcttctcctc tctctccgta tctcttccag ctgagatcga cgccttcgaa gaggtcgctc
     1741 gtcgccgggt cgcccgccgt cagcttgaag tcgttcatcc acgtgcctcg gcgctcggcg
     1801 gcgccgaagc cctggctctt gtgcatggag cggctctgcc cggcgatctc cgtgtacgag
     1861 cggccgagga gcgcgttgta gccgccgaga tcgagcgcga tctgcccggg agcggcgttg
     1921 cgcggtgcgt cggagccgaa gcgaaagacg ttccagaaga tgcgcctcgc ctgccagggc
     1981 cggacccacc ggagctgctc ggggaatcgc gtcggatcgg cggcggcggc gaacgcctcg
     2041 cccgcgagga ttgccgaggc cgtgtggttg ccgtgacccc cctcgcccgt cgtcgggaag
     2101 cgcgtcacga tcacgtcggg gcggaaggac cggatgatcc acacgacgtc ggacaggacc
     2161 tgctcgtggc cccagatcgc gaacgtctcc tcggggttct tcgagtagcc aaagtcgatc
     2221 gcccgcgtga agaactggcg ggcgccgtcg atgcgccgcg ccgcgaggag ttcctgcgtg
     2281 cgcaggacgc cgacgagctc tccggtctcg ttcccgatga ggttctggcc gccgtcgccg
     2341 cgcgtgacgg agagatagcc ggtttcgagg agccggccct tcgcgcccca tgccaggaac
     2401 gccgtgttct cgtcgtccgg gtgagccgcg acgtagagga cgctgccgac gacgttcagc
     2461 ttcttcagct ggagagcgat ctccgaggcc gagagggtct cggagggtgc ggcggcgcgc
     2521 gccgcgccgg aggcgagaag gaggacggcg gcggcgaggg agcggagcgg gcggcgcgtc
     2581 atgcgccgca ttctagaggg ggagtctcaa tccgcggcgt gcttcccgaa cgtctcccgg
     2641 acgaagaacg tcccggcgca cgcgacgagg agcgccccga gggacgcgag gaacgccgcg
     2701 cggtagcccg ccgtggggta gcggcgggcc ccctgctcga tgacgccgag ccagtgggag
     2761 tccaggatgc gtccgaggac gttctgcatg acggccgccc cgaggaagcc gccggcgttc
     2821 acggtggccg tggcgatccc ggagaagcgc ggcggattca cctcgcgggc gagcacccac
     2881 gtgagcgtga agcagctcgc cgtgaggccg acgaggaagc agagcgggcc gagaagggcg
     2941 cgcggaggtc cgccggtcgg gacggcgacg cacgcccaga cggccgtgta cacgaacgtg
     3001 aagaggacgt acggcagccg gcgcgcgcgg aagacgcggt cggacaggaa cccggcggcg
     3061 ggcgcgccga agacgacgcc gactccgacg agcgacacga gctgcgcggc ctccttcgcc
     3121 gagagcccgt agacgtcgcg catccacggg acggcccaga gccccgagaa cgagatcagc
     3181 gtcccgtact ggcagaagaa gacgaggaac atcggccacg tcctccggtt gccccagacc
     3241 gagcggtagc cctgcacgac gctcagcttc ggcacggggc cgcgcgggag gggcggcggg
     3301 aagccggcgt cctcgggccg gtcgcggacg acggcgagcg tgaggagtgc gagcgcgacg
     3361 gaaagcgccg cgacgccgcc gaacgtcgtc cgccagcccg cgagggagac gacgagcgcg
     3421 agcggcgcgg ccgccatcag gccgccgagg ttgccggcca cctgcgtcag gcccgaccac
     3481 gtcccgaact cgtcgggccg gaaccacgcg ccgatctgct tcagcgttcc gatgaagacg
     3541 acggacgctc cgacgccgac gacgacgcgc gcgccgtagg cgagcggcag ggcgtgcgcc
     3601 gccgcgaaga ccgcgccccc cacgcccatc acgagcgcgc ccgcggcgac ggccttgcgc
     3661 ggtccccacg cgtccacggc agcgccgacg acgaactgca tcgcggcgaa cgcgtagagg
     3721 tagagcgccg ccatcgtcgc gacctcgacg cccgtcgccg cgaagctccg catgaggtcc
     3781 ttcgcgagga cggcgggcgc gatccggtgg aagtacgaca cgacgtacac gagcgcgaga
     3841 acgccccaga cgaggaggcg gcgcgagcgg aaggcggaag aaacggcgat cctccttggc
     3901 ctcaggggtt ccacgtgaag cggacgggct gggcgattct cctccggccg tcggcggcgc
     3961 ggaacgtgac cttgagatcg tgagcgcccc cggcaagacc ggacgcgtcg agccttgccg
     4021 tgtagcctgc gcgcgaggcg tcgccgatct cgggaaccgc ggccgcgacg tccgggcgcg
     4081 gcgtgcgctc gagggcgagg acgcgcgcga ggaccccgtc cacgcggaac tcgacgggct
     4141 cgaccgcgcc gccgccgcgg agctggcacc atccgcggac gtcgagcgtc ccgccgagag
     4201 ccgcgccttc cgccggcgcg tcgaccgcca cggggatcga cgcgtcgtaa cggatcacgt
     4261 cgtcctcgcg gccgccaggc cgccttccga cggagagcct tcgcggcgcg atgccgatcc
     4321 gggtttcgag gaggaagccg agcgagatcc gggcgagaag cggatccggg cggccgaata
     4381 cccgttcgga cgcgagccct tccacgcgcc ggtccgccgt cagcacgacg tcgccttccg
     4441 ccgcggcgac ggcgcgatcg ggcgcgatct cgctctgcgc gcgctccggc aggaaacgcg
     4501 aaacgtgcat ccggagctcg gggtcgaaga cgagcgtccc gcggtcgtac gggggctgcg
     4561 aggcggcgcg gacggcggag gcggaggggc tcggcgccga cgcagccgcg acgggcggaa
     4621 ccgcgaccca cgcgcacgcg gccacgaggg cgacggatgc acaggctccg gggcgcggga
     4681 cccggcttgc gagcgcggcg agaccgcacg cggcgagccc cgcgacgccg cccaggagga
     4741 gcgctccgta gcgcggcgcg ccgtagagcg tggagacggg cacgttcacg agcgtccacg
     4801 gcaggacgag aaggaggatg cgcgacgcgg ttcgccggcg cagcagggcc cccgcggccg
     4861 cggcggcgcc gaccacggcg ccgcgcgtgt cggagccgaa cgggtcgacg agccagcgcc
     4921 gccagaggga gagcgagaga aggtccctcg ccgagagcgc cgagctgcgg cggaggtccg
     4981 ccgcctggat gaggagctgc tcgcggaacg gcgcgaatcc gcccgagccg cggatcgcgg
     5041 gcagccaggc g
//
LOCUS       DAOMFY010000286         5049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5862_length_5049_cov_8.301562, whole genome shotgun sequence.
ACCESSION   DAOMFY010000286 DAOMFY010000000
VERSION     DAOMFY010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5862_length_5049_cov_8.301562"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLB01_16855"
     CDS             <1..799
                     /locus_tag="PLB01_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ79025.1"
                     /translation="AVGVLVDTTRCVGCRGCEAACSEANALAAPDMPGDDAVFAARRR
                     TGPTSFTVVNKSTAKSPAGEDRYAKTQCMHCVTPACASGCVVKALEKTASGPVVYHED
                     RCLGCRYCMIACPFSVPKYEYTKAAPLVKKCTFCAARQEKGLPPACTDACPSGALTFG
                     KRADLLEEAKTRIYQNPTKYVHEIYGEDEAGGTSWLYISDVSSASLSLPQDIVKKSYP
                     EMADGALSAVPFVVTLWPPLLMGLYTFSKRVGDVAGRESAHKGDGHE"
     gene            792..2015
                     /gene="hybB"
                     /locus_tag="PLB01_16860"
     CDS             792..2015
                     /gene="hybB"
                     /locus_tag="PLB01_16860"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HQQ79026.1"
                     /translation="MSETFDRVAASAFLREKVFLGMEPKTYAKSLFTKTNAIFAAILA
                     VGLPTMAYRFWHGMGAVSNLSQTNPWGIWVAFDIVCGVALAAGGYTVAAAVYLFGQKQ
                     FAPVLRPAILTGFLGYAFVLVGLLVDLGQPWRLPYQFFLTPGTTAVMYEVGWCVFLYL
                     MVLALEFLPAALEWLGMTKVRRFMSAISIGIIVLGVTLSTLHQSSLGAFFLMSPGKLH
                     PLWYSPFLPIFFFVSSIAAGIGMVIFESSVSHRAFQDRLDPEAHVDMDALQLGLAKGG
                     AVVLFAYFFIRLQGLVASGRFDLLLTGWGAWFALEMVGFVLLPSLLFAFAVRTQNAKL
                     ARAAGVMTVLGVVLNRFNVSLIAFNWNVPDRYVPALGEIVISITIVTLGVLVFRWIVN
                     RMPVLREHPDWRGAH"
     gene            2033..2698
                     /locus_tag="PLB01_16865"
     CDS             2033..2698
                     /locus_tag="PLB01_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HQQ79027.1"
                     /translation="MNESSLLSPHATKALEYLLAVSYLLLFIPFWRFVNGATIAQSFA
                     LGWFQVPENVHLHRGHSWAKTFGGAVAVGLDDFAHKLIGPLDEVRFPAVGTPLRQGQK
                     AFTVVADGKPFDVVSPVDGNVVAVNEVVHAHPADAVRDPYGTGWLLKVEPRWLTANLK
                     NLVSGDSARRFLDAAAQALSGRMSPELGVVLQDGGAPVHGIAREIDPEHWDEVVRHHL
                     KGE"
     gene            2709..3059
                     /locus_tag="PLB01_16870"
     CDS             2709..3059
                     /locus_tag="PLB01_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ79028.1"
                     /translation="MKARIHFLAATLLLALLSATLLAGALPNLPKEKALPQGDGSPGV
                     VTFRHDSHVDASRPDCTTCHPTLFPIVKRTASSAAPAAIRHADMEKGKSCGSCHDGKS
                     AHGLDDCGTCHKAK"
     gene            3068..3766
                     /locus_tag="PLB01_16875"
     CDS             3068..3766
                     /locus_tag="PLB01_16875"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HQQ79029.1"
                     /translation="MTPLEFLQLAGIFLAGLVVRALAFTLVLAVCALPILAAWALWRA
                     WQKLRDRSIGLVDVGGLELAEAHHYAPGHTWLQRRARGSVRVGLDEIAGRLLHGPTSV
                     TLPRPGTVLAAGLPAATVSCHGRAAAIPAPISGTVVATNPAVVRDPSLLEESRYRDGW
                     LFAMKPATAAFLTLPTGETARSWFKDETARFSRALETELGLLAADGGELVVEAPALLS
                     EEKWQRLVSEFLKG"
     gene            complement(3770..4216)
                     /locus_tag="PLB01_16880"
     CDS             complement(3770..4216)
                     /locus_tag="PLB01_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79030.1"
                     /translation="MGDRKYRSKGYQDGARTGGSGGTGGGGGFSSDRPARLEGAPRGR
                     GADRHRDEVFRCKACGEKNDAEVTKASVCRKCGAALHACSQCRHFDGAAPAQCRQPVP
                     VAIPAKSRANDCTFYAPVVSMDLTGTKAADTPDGARSAFDSLFGKR"
     gene            4273..4635
                     /locus_tag="PLB01_16885"
     CDS             4273..4635
                     /locus_tag="PLB01_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQQ79031.1"
                     /translation="MRLLLRILINAAALWVAASVVDGIHAGGAGSILAVAVVFGVVNA
                     LVRPILKLLSCPIIFLTLGLFTLVLNALMLMLTAWASRQLGIDFTVAGFWPSAFLGAL
                     IVSIVSTVLSWFIPDKAA"
     gene            4665..>5049
                     /locus_tag="PLB01_16890"
     CDS             4665..>5049
                     /locus_tag="PLB01_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HQQ79032.1"
                     /translation="MRVPVTLMALVLLAGCGKQQPALDVKTAAPAKAPAASASAPASG
                     VMPAAPEAALVTVSGTVLETMDAAEYTYMRLKTATGETWAAINKTKVAKGDAVTVTNA
                     MVMDGFQSKTLNRTFDHILFGVLATP"
BASE COUNT          693 a         1913 c         1619 g          824 t
ORIGIN      
        1 cgccgtcggc gttctcgtcg acacgacgcg ctgcgtcggc tgccgcggct gcgaggccgc
       61 gtgctccgaa gcgaacgcgc tcgccgctcc cgacatgccg ggcgacgacg ccgtgttcgc
      121 cgcgcgccgc cggacgggac ccacgagctt cacggtcgtg aacaagtcga ccgcgaagag
      181 ccccgcgggc gaggaccgct acgcgaagac gcagtgcatg cactgcgtga cgcccgcctg
      241 cgcgtcgggc tgcgtcgtga aggcgctcga gaagacggcc tcggggccgg tcgtgtacca
      301 cgaggaccgc tgcctcgggt gccgctactg catgatcgcg tgccccttca gcgtgccgaa
      361 gtacgagtac acgaaggcgg cgcccctcgt gaagaagtgc acgttctgcg cggcgcgcca
      421 ggagaagggc cttccgccgg cctgcacgga cgcgtgcccc tcgggcgcgc tcacgttcgg
      481 caagcgggcc gacctcctcg aagaggccaa gacccgcatc taccagaacc cgacgaagta
      541 cgtccacgag atctacgggg aggacgaggc cggcggcacg agctggctct acatctccga
      601 cgtctcctcg gcgagcctct cgctcccgca ggacatcgtg aagaagtcct accccgagat
      661 ggccgacggc gcgctctcgg ccgtcccgtt cgtcgtgacc ctgtggccgc cgctcctcat
      721 gggtctctac accttcagca agcgggtggg cgacgtcgcc ggccgcgaga gcgcgcacaa
      781 gggagacggc catgagtgag accttcgacc gtgtggcggc ttcggccttc ctccgcgaaa
      841 aagtctttct cggcatggag ccgaagacgt acgcgaagag cctcttcacg aagacgaacg
      901 cgatcttcgc ggcgatcctc gccgtcggcc tgcccacgat ggcctacagg ttctggcacg
      961 gcatgggcgc ggtgagcaac ctctcgcaga cgaacccctg gggcatctgg gtggcgttcg
     1021 atatcgtgtg cggcgtcgcg ctcgccgccg gcggctacac cgtggccgcg gcggtctacc
     1081 tgttcggaca gaagcagttc gccccggtcc tccgccccgc gatcctcacg ggcttcctcg
     1141 gctacgcctt cgtcctcgtc ggcctgctcg tcgatctcgg gcagccgtgg cggctcccgt
     1201 accagttctt cctcacgccc ggcacgaccg ccgtcatgta cgaggtcggc tggtgcgtct
     1261 tcctgtacct gatggtcctc gcgctcgagt tccttccggc ggcgctcgag tggctcggga
     1321 tgacgaaagt tcgccgattc atgagcgcga tctcgatcgg gatcatcgtc ctcggcgtca
     1381 cgctctcgac gctccaccag tcctcgctcg gcgccttctt cctcatgtcg cccggcaagc
     1441 tccaccccct gtggtactcg ccgttcctcc cgatcttctt cttcgtgtcc tcgatcgcag
     1501 cgggcatcgg gatggtgatc ttcgagagct cggtctcgca ccgggccttc caggaccggc
     1561 tcgaccccga ggcgcacgtc gacatggacg cgctgcagct cgggctcgcc aagggcggcg
     1621 cggtcgtcct cttcgcgtac ttcttcatcc gcctgcaggg cctcgtcgcg tccggccggt
     1681 tcgacctcct cctgaccggg tggggcgcct ggttcgcgct cgagatggtc ggcttcgtcc
     1741 tcctgccgtc gctcctcttc gccttcgccg tgaggaccca gaatgcgaag ctcgcgcgcg
     1801 ccgccggcgt catgacggtg ctcggcgtcg tcctcaaccg cttcaacgtg tctctgatcg
     1861 cgttcaactg gaacgtgccg gaccggtacg ttcccgcgct cggagagatc gtcatctcga
     1921 tcacgatcgt cacgctcggc gtcctcgtgt tccgctggat cgtcaaccgc atgcccgtgc
     1981 tccgcgagca tccggactgg cgcggcgccc actgaaagag cggagaagaa acatgaacga
     2041 aagcagcctc ctcagcccgc acgccacgaa ggccctcgag tacctgctcg cggtctcgta
     2101 cctcctgctc ttcatcccct tctggcgctt cgtgaacggc gcgacgatcg cgcaatcctt
     2161 cgcgctcgga tggtttcagg tccccgagaa cgtccacctg caccgcgggc actcgtgggc
     2221 caagacgttc ggcggcgccg tcgcggtcgg cctggacgac ttcgcccaca agctgatcgg
     2281 cccgctggac gaggtgcggt tccccgcggt cggcacgccg ctccggcagg gccagaaggc
     2341 cttcacggtc gtcgcggacg gcaagccctt cgacgtcgtc tcgcccgtgg acggcaacgt
     2401 cgtcgcggtc aacgaggtcg tgcacgcgca cccggccgac gccgtccgcg atccgtacgg
     2461 aacgggctgg ctcctgaagg tcgagccgcg ctggctgacc gcgaacctca agaacctcgt
     2521 ctccggcgac tccgcccgcc gcttcctgga tgcggccgcc caggcgctct cgggccgcat
     2581 gagcccggag ctcggcgtcg tcctccagga cggcggcgcc ccggtccacg gcatcgcccg
     2641 cgaaatcgac cccgagcact gggacgaggt cgtgcgccac cacctgaagg gcgagtgagg
     2701 aggacgcgat gaaagcccgc atccatttcc tcgcggcgac cctccttctc gcccttctct
     2761 cggcgaccct cctcgcgggc gcgctgccga acctcccgaa ggagaaggcg ctcccgcagg
     2821 gcgacggcag ccccggcgtc gtcaccttcc gccacgactc ccacgtcgac gcctcgcgcc
     2881 ccgactgcac gacgtgtcac ccgacgctct tccccatcgt caaacgcacc gcctcttcgg
     2941 ccgcccccgc cgcgatccgc cacgccgaca tggaaaaagg caagagctgc ggctcctgcc
     3001 acgacggcaa gagcgcccac ggcctcgacg actgcgggac gtgccacaag gccaagtgag
     3061 gtgacccatg acgcctcttg agttcctcca gctcgccggc atcttcctcg cgggtctcgt
     3121 cgtccgcgcc ctcgccttca cgctcgtcct cgcggtctgc gcgctcccga tcctcgcggc
     3181 ctgggcgctc tggcgcgcct ggcagaagct gcgggaccgc agcatcggcc tcgtggacgt
     3241 gggcgggctc gagctcgccg aggcgcacca ctacgccccc ggccacacgt ggctccagcg
     3301 gcgggcgcgc ggctcggtcc gcgtcgggct cgacgagatc gcgggccggc tcctgcacgg
     3361 gcccacgagc gtcacgctcc cgaggccggg caccgtcctg gccgccggcc tccccgccgc
     3421 caccgtgagc tgccacgggc gcgccgccgc gattccggcg cccatctcgg gcacggtcgt
     3481 cgcgaccaac ccggccgtcg tccgcgaccc ctcgctcctc gaggagtcgc gctaccggga
     3541 cggctggctc ttcgcgatga agcccgcgac ggccgcgttc ctgacgctcc cgaccggcga
     3601 gaccgcgcgc tcctggttca aggacgagac ggcgcgcttc tcgcgcgcgc tcgagaccga
     3661 gctcggcctc ctcgccgcgg acggcggcga gctcgtggtc gaggcgcccg cgcttctctc
     3721 ggaggagaag tggcagcgcc tcgtctcgga gttcctgaag ggctgacgct cagcgcttgc
     3781 cgaagaggct gtcgaaggcc gaccgcgcgc cgtcgggcgt gtcggcggcc ttcgtgcccg
     3841 tgaggtccat gctcacgacg ggcgcgtaga acgtgcagtc gttcgcccgc gacttcgccg
     3901 ggatcgcgac cgggaccggc tggcggcact gcgcgggagc tgccccgtcg aagtggcggc
     3961 actggctgca tgcgtggagc gcggccccgc acttccggca gaccgaggct ttcgtcacct
     4021 cggcgtcgtt cttctcgccg caggccttgc agcgaaagac ttcgtcgcgg tggcggtccg
     4081 cgccgcgccc gcggggcgcg ccttccagcc gggcggggcg gtcggaggag aacccgccgc
     4141 ccccgccggt gccgccagag ccacccgtgc gcgcgccgtc ctgatagccc ttcgagcggt
     4201 acttgcgatc acccatccgc atcattctac ggtccggcgc gcgggccgta gaatcctccg
     4261 ccgagggact ccatgagact cctccttcgc atcctcatca acgcggcggc cctgtgggtc
     4321 gcggcgtcgg tcgtggacgg catccacgcc ggcggggcgg gctccatcct cgcggtcgcg
     4381 gtcgtcttcg gcgtcgtgaa cgcgctcgtg cgcccgatcc tcaagctcct gagctgcccg
     4441 atcatcttcc tgacgctcgg gctcttcacc ctcgtcctca acgcgctcat gctcatgctc
     4501 acggcgtggg cctccaggca gctcgggatc gacttcacgg tcgccggctt ctggccctcc
     4561 gccttcctgg gcgcgctcat cgtctccatc gtctcgacgg tcctgtcctg gttcatcccg
     4621 gacaaggccg cgtgatccaa cacccgaaaa ggagctcttt ccccatgcgc gttccggtca
     4681 ccctgatggc tctcgttctc ctcgccggct gcggcaagca gcagcccgcg ctcgacgtga
     4741 agaccgcggc gcccgcaaag gctcccgcgg cgtccgcgtc cgcccccgcg tccggcgtca
     4801 tgcccgccgc gcccgaagcg gcgctcgtca ccgtctccgg caccgtcctc gagacgatgg
     4861 acgcggccga gtacacctac atgcgcctga agaccgcgac cggcgagact tgggccgcga
     4921 tcaacaagac gaaggtcgcg aagggcgacg ccgtcacggt cacgaacgcg atggtcatgg
     4981 acggcttcca gagcaagacg ctcaatcgca cgttcgacca catcctcttc ggcgtgctcg
     5041 cgacgcccg
//
LOCUS       DAOMFY010000287         5011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5939_length_5011_cov_8.458434, whole genome shotgun sequence.
ACCESSION   DAOMFY010000287 DAOMFY010000000
VERSION     DAOMFY010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5939_length_5011_cov_8.458434"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLB01_16895"
     CDS             complement(<1..486)
                     /locus_tag="PLB01_16895"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQQ79033.1"
                     /translation="MSEPAAFPRPHAGILPPKALLLSLVAQLPLLALRWPPRPDAWQL
                     AAGASLLLAGAVLNVWAERLFRRDGVGVCPFTDAPRVVVEGPYRISRHPMYLGLVFLC
                     VGACLLTGVLWNLWAPAALLAYLHVRFVLREEEFLVARLGAEYERYRGRHARWLGLPP
                     RD"
     gene            complement(479..934)
                     /locus_tag="PLB01_16900"
     CDS             complement(479..934)
                     /locus_tag="PLB01_16900"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQQ79034.1"
                     /translation="MSVSPDLLEETCWEIRRAFRDLAAAADAELAPLGIRAADRALLE
                     WIVREKGAVSLSELARKRSVSRQYIHQMWKRLPDPGWIQARPDPDDRRSLLLSLSPKG
                     RAFWKRVRTADAAFFARLSPAWTRAETESVQRALRRLRAALRPDGADDV"
     gene            complement(1043..1543)
                     /locus_tag="PLB01_16905"
     CDS             complement(1043..1543)
                     /locus_tag="PLB01_16905"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQQ79035.1"
                     /translation="MKRPARVVLGGLLLAAAACAADRGSTSAAPAKSPAPAVSGPRVV
                     MPNGAVISVEIATTDAEKAQGLMFRESMPRNAGMIFPFEGLEIRPFWMKNCHFPLDLI
                     YATKDGTVVEVIKSLPPCPPDPAPCPNTAPKEKSDTVLEVNAGVADATGAVPGAKLKW
                     VEIPGR"
     gene            complement(1540..2982)
                     /locus_tag="PLB01_16910"
     CDS             complement(1540..2982)
                     /locus_tag="PLB01_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ79036.1"
                     /translation="MTAETGPHLLVVDDEAGIREMLTIVFQKEGYRVSAARGCVEGLA
                     LLDGEAPDLVLTDLKMPDGTGFDILKKVREAHRDTPVVMITAYSSTKTAVDALKQGAY
                     DYIAKPFDVDELKHVVARALERKRLVEENVALKERADGRGMGPVVGVSRKMRALFELV
                     ARIGKTTSTVLITGDSGTGKELVARAIHAASARAGRPFISINCGAMPENLLESELFGH
                     ERGAFTGAVKEKKGLFHEAEGGTLFLDEIGETSLTMQVKLLRALQERVVRRVGGNAEE
                     PIDVRIICATNKDLQKKVAEGAFREDLYYRINVIPVLLPPLRERRDDVPHLVRHFLRK
                     VSVEQGIPEKRITTEAMRLLEAYAWPGNVRELENLIERTVALEPGDVITTASLPDTFL
                     HPAGLPPASSMEGFDLPADGLDLEAYLEWLGKRLMQQALERTGGVQIRAAELLRMSER
                     SFRYYAKKYAIRREGEESAPPPEAVEEVES"
     gene            complement(2979..4673)
                     /locus_tag="PLB01_16915"
     CDS             complement(2979..4673)
                     /locus_tag="PLB01_16915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ79037.1"
                     /translation="MSSSRTRAFEPLHSMLRGFVVLRVVVVSTILLSAFLIQFTFSTA
                     LPLNDIYYLAAFAYSISIAAILSLDRIPPEANAAIQLLGDLVVITGLVYISGGADSSF
                     SFLYLGTVAAGAILLGRQGGLVAAGLASVFYAVLVDLMYFGALKPVPSTERTPHLWTP
                     AGLVGQVALHAGAFVVTALLVSYSSNKLRETRTDLERRKSEIARLQALHGSVLGSMSS
                     GVLTTDPDGLVTFANRAAQELLGVPSVDLVGRPVQSLGLIGEAAWKRIAAAGSEILRF
                     ETTRRIKDQDAYFGVSSSALRDGSGYTVGRILIFQNVTHVKRLEGEVRLKDKLAAVGE
                     LAAGIAHEIRNPLASISGSVQALQGSVPSDSPEHRLMKIVVAESHRLSTILEDFLRYV
                     RPKERAVEPVDAPAALRDVLTLLAHSDEVSSRHAIEVRTAPDSFVLPADPGQLRQIFW
                     NVARNALAAMPGGGTLTVTATLEGAVWKVSFADEGHGMTEDERERLFTPFAHSFPGGT
                     GLGLAIVHRIVEEHGGSIEVDSAPGRGATVAISLPRNDASTGARPTETALAAAEVP"
     gene            complement(4773..>5011)
                     /locus_tag="PLB01_16920"
     CDS             complement(4773..>5011)
                     /locus_tag="PLB01_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQQ79038.1"
                     /translation="TKVFPPMVVQMIGVGEQTGALDAMLNKIAEFYEEEVDNAVTGLM
                     KLMEPIMIAILGTVIGTIVVAMYMPMFDLISKIN"
BASE COUNT          800 a         1635 c         1834 g          742 t
ORIGIN      
        1 atcgcggggg ggaagcccga gccatcgggc gtggcggccg cggtagcgct cgtattccgc
       61 cccgagccgg gcgacgagaa actcctcctc gcgcaggacg aaccgcacgt ggagatacgc
      121 gaggagcgcc gcgggggccc agagattcca gaggacgccc gtcagaaggc acgccccgac
      181 gcaaaggaag acgagcccga ggtacatcgg gtgccgcgag atccggtatg ggccctccac
      241 gacgactcgc ggcgcgtccg tgaagggaca gacgccgacg ccgtcccggc gaaagagacg
      301 ctcggcccag acgttgagca cggcgcccgc gagaagaagt gaagcgccgg ccgcgagctg
      361 ccacgcgtcc gggcggggag gccatcggag cgcgaggagc gggagctgcg cgacgaggct
      421 caggagaagg gccttcggcg gcaggatgcc ggcgtggggg cgggggaagg cggcgggttc
      481 agacatcgtc ggctccatcg gggcggagcg ccgctctcag gcggcggagc gctcgttgga
      541 cggattcggt ctccgcccgc gtccacgccg gggaaaggcg ggcgaagaac gccgcgtctg
      601 ccgtgcgcac tctcttccag aacgcccggc ccttcggcga aagcgagaga aggaggcttc
      661 ggcggtcgtc ggggtccggg cgcgcctgga tccaacccgg atcggggagg cgcttccaca
      721 tctgatggat gtactggcgg ctcacggacc gcttccgcgc cagttcggac agggaaaccg
      781 cgcccttttc gcgaacgatc cactcgagaa gggcccggtc cgccgcccgg attccgagcg
      841 gggcgagctc cgcgtcggcc gcggccgcga ggtcgcggaa ggcccgccgg atctcccagc
      901 acgtctcttc gaggaggtcg ggggaaacgc tcatgatcgt caatatggat tgacgaacaa
      961 tgtttgtcaa gtagtattga cgaatcgcag cggtcccgct tttcccggaa tccggagagc
     1021 ctcgaatcga taatacgagc cgctatcgcc ccgggatctc cacccacttc agctttgcgc
     1081 cggggacggc accggtcgcg tccgcgacgc cggcgttgac ctcgaggacc gtgtcggact
     1141 tctcctttgg cgcggtgttc gggcagggcg cggggtcggg cggacagggc gggagggact
     1201 tgatgacctc gacgaccgtg ccgtccttcg tcgcgtagat gaggtccagc gggaagtggc
     1261 agttcttcat ccagaacggc cggatctcga gcccctcgaa cgggaagatc attcccgcgt
     1321 tcctcggcat cgactctcgg aacatcagcc cctgcgcctt ctcggcgtcc gtcgtcgcga
     1381 tctcgacgga gatgacggcg ccgttcggca ttacgacgcg gggtccggag accgccggcg
     1441 ccgggctctt cgcgggtgcg gcggacgtcg agccgcggtc ggccgcgcag gccgcggccg
     1501 caaggagcag gcccccgaga acgacgcgcg ccggccgctt cacgactcga cttcctcgac
     1561 cgcctcgggc ggcggcgccg actcctcgcc ctcgcgccgg atcgcgtact tcttcgcgta
     1621 gtagcggaag gagcgctcgg acatccggag cagctcggcc gcgcggatct ggacgccgcc
     1681 cgtcctctcg agcgcctgct gcatcagacg cttgccgagc cattcgaggt acgcctcgag
     1741 gtcgaggccg tcggcgggga ggtcgaagcc ctccatcgag gacgcgggcg ggaggcccgc
     1801 ggggtggagg aacgtgtccg ggagcgaggc cgtcgtgatg acgtcgcccg gctcgagggc
     1861 gaccgtgcgc tcgatgaggt tctcgagctc gcggacgttg ccgggccacg cgtacgcctc
     1921 gaggagccgc atcgcctccg tcgtgatgcg cttctcgggg atgccctgct cgaccgagac
     1981 ctttcgcagg aaatgacgca cgaggtgggg cacgtcgtca cgtcgctcgc gcagcggagg
     2041 gaggaggacg gggatcacgt tgatgcggta atagaggtcc tcgcggaagg cgccctcggc
     2101 gaccttcttc tggaggtcct tgttcgtcgc gcagatgatc ctcacgtcga tcggctcctc
     2161 cgcgttgccg ccgacgcggc ggacgacgcg ctcctggagc gcccggagga gcttgacctg
     2221 catcgtgaga gacgtctcgc cgatctcgtc gaggaagagc gtgccgccct cggcctcgtg
     2281 gaagaggccc ttcttctcct tgacggcgcc cgtgaacgca ccgcgctcgt ggccgaagag
     2341 ctcggattcg aggaggttct cgggcatcgc gccgcagttg atcgagatga acggccgccc
     2401 cgcgcgcgcg gaagcggcgt ggatcgcgcg cgcgacgagc tccttgcccg tgccggagtc
     2461 gcccgtgatg aggacggtcg acgtagtctt gccgatccgg gccacgagct cgaagagtgc
     2521 gcgcatcttc cgcgaaacgc ccacgacggg gcccatcccg cgcccgtcgg cgcgctcctt
     2581 gagcgcgacg ttctcctcca cgaggcgctt gcgctcgaga gcgcgtgcga cgacgtgctt
     2641 gagctcgtcg acgtcgaacg gcttggcgat gtagtcgtac gcaccctgct tgagggcgtc
     2701 cacggcggtt ttcgtcgacg agtacgccgt gatcatgacg accggcgtgt cgcggtgggc
     2761 ctcgcgcacc ttcttgagga tgtcgaagcc cgtcccgtcg ggcatcttga ggtccgtgag
     2821 gacgaggtcg ggtgcctcgc cgtcgaggag cgcgagccct tccacacagc cgcgtgccgc
     2881 cgagacgcgg tagccctcct tctggaagac gatcgtgagc atctcccgga tgccggcctc
     2941 gtcgtcgacg acgaggagat gggggccggt ttcggccgtc atggaacctc cgcggcggcg
     3001 agcgccgtct ccgtgggtcg cgcgccggtc gacgcgtcgt tgcgcgggag cgagatggcg
     3061 acggtcgccc cgcggccggg cgccgagtcg acctcgatgg agccgccgtg ctcctcgacg
     3121 atccggtgga cgatcgcgag gcccaggccc gttccgcccg ggaagctgtg ggcgaacggc
     3181 gtgaagaggc gctcgcgctc gtcttccgtc atcccgtggc cctcgtcggc gaacgagacc
     3241 ttccagacgg cgccctcgag cgtggcggtc accgtgagcg tgccgcctcc gggcatcgcg
     3301 gccagagcgt tgcgcgccac gttccagaag atctgccgga gctggccggg gtcggccgga
     3361 aggacgaacg agtccggcgc cgtccgcacc tcgatcgcgt ggcgcgagga gacttcgtct
     3421 gagtgcgcga gaagcgtcag aacgtcgcgg agcgcggccg gggcgtcgac gggctcgacc
     3481 gcccgctcct tcggcctcac gtaccggaga aagtcctcga ggatcgtgct caggcggtgc
     3541 gactcggcga cgacgatctt catgaggcga tgctccggcg agtcggacgg caccgagccc
     3601 tgaagcgcct gcaccgagcc cgagatcgac gccagggggt tgcggatctc gtgcgcgatt
     3661 ccggccgcga gctcgccgac ggccgcgagc ttgtccttga gccgcacctc gccctcgagg
     3721 cgcttcacgt gcgtgacgtt ctggaagatg aggatgcgcc cgacggtgta gcccgacccg
     3781 tcccggagcg ccgacgacga cacgccgaag taggcgtcct ggtccttgat gcggcgggtc
     3841 gtctcgaacc ggaggatctc cgagccggcg gccgcgatcc gcttccaggc ggcctccccg
     3901 atgaggccga gcgactggac gggacggccg acgaggtcca cggagggcac gccgagcagc
     3961 tcctgcgcgg cccggttcgc gaacgtgacg agcccgtcgg ggtccgtcgt gaggacgccg
     4021 gaggacatcg agccgaggac cgacccgtgc agggcctgca gccgcgcgat ctcggacttg
     4081 cgccgctcga ggtccgtccg ggtctcgcgg agcttgttcg acgagtagga cacgagaagc
     4141 gccgtcacga cgaacgcgcc ggcgtgaagg gcgacctggc ccacgagccc ggccggagtc
     4201 cagaggtgcg gcgtgcgttc ggtcgacggc acgggcttca gcgcgccgaa gtacatgagg
     4261 tcgacgagga cggcgtagaa gaccgaggcg aggcccgccg cgacgaggcc gccctggcgg
     4321 ccgagcagga tcgccccggc cgcgacggtg ccgaggtaga ggaacgagaa gctcgagtcg
     4381 gcgccgcccg agatgtagac gagtcccgtg atcacgacga ggtcgccgag gagctggatc
     4441 gcggcgttcg cctcgggcgg gatcctgtcg agggagagga tcgcggcgat cgagatcgag
     4501 tacgcgaacg cggccaggta gtagatgtcg ttgagcggca gcgccgtcga gaacgtgaac
     4561 tggatcagga acgcggagag gaggatcgtc gagaccacga cgacgcgcag gacgacgaac
     4621 ccccgcagca tcgagtgcag cggctcgaac gcacgagtgc gtgaggaaga caccatagga
     4681 ttttagttct tagagagggt taagggggga acggggggat gcgaacccgg cggctgcttc
     4741 gcgcaggcgg acgacgcatc cccccgaaca ggtcagttga tcttgctgat gaggtcgaac
     4801 atcggcatgt acatcgcgac gacgatcgtg ccgatgaccg tgccgaggat cgcgatcatg
     4861 atcggctcca tgagcttcat gaggcccgtg accgcgttgt cgacctcctc ctcgtagaac
     4921 tcggcgatct tgttgagcat cgcgtcgagc gcgcccgtct gctcgccgac gccgatcatc
     4981 tggacgacca tcggcgggaa gaccttcgtc t
//
LOCUS       DAOMFY010000288         4998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_5957_length_4998_cov_8.724661, whole genome shotgun sequence.
ACCESSION   DAOMFY010000288 DAOMFY010000000
VERSION     DAOMFY010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5957_length_4998_cov_8.724661"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLB01_16925"
     CDS             complement(<1..957)
                     /locus_tag="PLB01_16925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79039.1"
                     /translation="MTDGNLAPGTRFGDYEILAELGAGGMGRVYRAKDLTLERLVALK
                     TLAPQFGSDTGFVQRFLKEARAAARLNHPNIVQIYDFGQVGETYYLAMEYVDGHSLGA
                     YLRRGHFSERDAILVIRHACRALAVAHAEGLVHRDIKPDNLMLTSRGEVKLVDLGIAK
                     RVDEDQSLTQTGQAVGTPHYISPEQIRGMRDVDARADIYSLGATLYHLVTGHTPFRGT
                     SGALVMSMHLAQPLADPRTYVAGLSEGLCRVIRKMMAKDRIERYADVDTLDRDLYRLQ
                     TGEVPEGAEPGDTAVDTVARDDVRAASGRPGSAPTHTPAPTSG"
     gene            complement(954..1829)
                     /locus_tag="PLB01_16930"
     CDS             complement(954..1829)
                     /locus_tag="PLB01_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HQQ79040.1"
                     /translation="MSLFVKFNLILILVFAVALVPAAYLSNQLLQTNARTQVVQNARL
                     MMQTALATRGYTNKQIKPLLAPRLAEEFLPQSVPAYSATEIFNNLRESNPEYTYKEAT
                     LNPTNPRDRTVDWEADVVNAFRADPKLTEVIGEREGPLGRSLHLARPIKITDAGCLSC
                     HTTPELAPASVVRAYGTNNGYGWKLNETVGAQIVTVPMSVPFAMATKAFQTLLATLVG
                     VFVFILVFLNILLWFVVIRPIRTLSSMADRISTGDLDVPEVAVRGRDEVAVLAGSFNR
                     MRISLTKALRMLEDE"
     gene            1951..2385
                     /locus_tag="PLB01_16935"
     CDS             1951..2385
                     /locus_tag="PLB01_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQQ79041.1"
                     /translation="MAANTRFAVGVHLLTSLAWSGGILTSEELAGSVVTNAVVLRRIL
                     SLLEKAGLVSGHPGRKGGFTLARPASAITLLDVHRAVGGKGLFAVHANKEARNCPVSA
                     CIKGVLAEVFTKAEGAAEGELRRVRLSDVVARVPSAGGKKGA"
     gene            2453..3073
                     /locus_tag="PLB01_16940"
     CDS             2453..3073
                     /locus_tag="PLB01_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQQ79042.1"
                     /translation="MKNTLALTAAAAAFAVSTAVAEPAVGPASAAGAWTIDKAHSEVG
                     FQVRHLMTKVRGSFGAFDGSVKIDAAKPEASTVSFVVKAASVNTNETKRDEHLRSADF
                     FDAASYPEIRFVSTSVRPAGENRYEVTGDLTLRGVTRKVSLPVVFLGVQKDPWGSEKA
                     GFETAVTLNRKDYGMVWNKALDHGGTILGDEVFVTIALEAARAKAN"
     gene            complement(3137..3562)
                     /locus_tag="PLB01_16945"
     CDS             complement(3137..3562)
                     /locus_tag="PLB01_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HQQ79043.1"
                     /translation="MPMPLVDLDAIPQVPLDFVNADHREEGRLLNAVADAVDALEAGL
                     GDAPAALASLAALRDHTAAHFGREDDAMRRSGFPAFPVHHAEHVRVLAELDDEIRRFR
                     ETGDAARLRAWVLGDVPQWFVNHIQTMDLVTARFVSGRG"
     gene            3668..4750
                     /gene="cax"
                     /locus_tag="PLB01_16950"
     CDS             3668..4750
                     /gene="cax"
                     /locus_tag="PLB01_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HQQ79044.1"
                     /translation="MKVLRFLLAFVPLALAAEWLFHSPALVFAFACLGLLPLAGAMGE
                     ATEQIAHRTGERVGGLLNATLGNAAELIITIVALRAGKVELVKASIVGSILGNLLLIL
                     GLSLLVGGLKNGLQRFDSRAAGTASTMMLLSVIGLLIPTLFEIVHKVQHHLPILSESP
                     DPLLDKLSLGVAAVLMLVYVLSLVFSLTTPSRGIAPEVGVTHREAPEGAWSLGAAVGV
                     LVGSTLATVVLSELLVGAVEPVVRRTGVSEVFLGIVLIPLVGNVAEHLVAVSTAAKNR
                     MELSLAISLGSSQQIALFVAPLLVFTSLLFGKEMTLFFNPFEVAVLGVSVLIAGQIAA
                     DGESNWLEGAQLLVVYAIVAMAFLWL"
     gene            4747..>4998
                     /locus_tag="PLB01_16955"
     CDS             4747..>4998
                     /locus_tag="PLB01_16955"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HQQ79045.1"
                     /translation="MRRLGAVLIAVGVVLLGAWAAARVHTVLGSRQDRSRFEEQRAAG
                     PRKRLVVLTFDDPRAFPWGGEPVLMDGRNVGEITSAGYSR"
BASE COUNT          710 a         1736 c         1725 g          827 t
ORIGIN      
        1 gcccgaggtc ggcgccggcg tgtgcgtcgg agcggagccc ggccgtccgg aggccgcccg
       61 cacgtcgtcg cgggcgaccg tgtcgaccgc cgtgtcgccg ggctcggccc cctccggcac
      121 ttccccggtc tgcagccggt agaggtcgcg gtcgagcgtg tcgacgtcgg cgtagcgctc
      181 gatccggtcc ttcgccatca tcttccggat cacgcggcag agcccttccg agaggccggc
      241 gacgtacgtg cgcgggtcgg cgaggggctg ggcgaggtgc atggacatca cgagcgcgcc
      301 ggacgtcccg cggaacggcg tgtggcccgt cacgaggtgg tagagcgtcg cgccgaggga
      361 atagatgtcg gcccgggcgt ccacgtcacg catgccccgg atctgttccg gcgagatgta
      421 gtgcggcgtg ccgacggcct ggcccgtctg cgtcagggac tggtcctcgt ccacgcgctt
      481 ggcgatcccg aggtccacga gcttgacctc gcctctgctc gtgagcatca ggttgtcggg
      541 cttgatgtcg cgatgaacga ggccctcggc gtgcgcgacg gcgagcgcgc ggcaggcgtg
      601 gcggatcacg aggatcgcgt cacgctcgct gaagtggccg cggcggagat acgcaccgag
      661 cgaatgcccg tccacgtatt ccatcgcgag gtagtacgtc tcgccgacct gcccgaagtc
      721 gtagatctgg acgatgttcg gatggttcag gcgcgccgcg gcgcgcgcct ccttcaggaa
      781 acgctggacg aagccggtgt cggacccgaa ctggggtgcg agggtcttga gcgcgaccaa
      841 gcgctcgagc gtcaggtcct tcgcgcggta gacacgcccc atcccgcccg ccccgagctc
      901 ggcgaggatc tcgtagtccc cgaagcgcgt tcccggcgcg aggttgccgt ccgtcactcg
      961 tcctcgagca tcctgagggc cttcgtgagg ctgatcctca tccggttgaa ggagccggcg
     1021 agcaccgcca cctcgtcgcg gccgcgaacg gccacctcgg gcacgtcgag gtctccggtc
     1081 gagatgcggt ccgccatgga cgagagggtc cggatcggcc ggatgacgac gaaccacagg
     1141 aggatattga ggaagacgag gatgaagacg aagacgccca cgagcgtcgc gagaagcgtc
     1201 tggaaggcct tcgtcgccat cgcgaacgga acggacatgg ggacggtgac gatctgcgcg
     1261 cccaccgtct cgttcagctt ccagccgtag ccgttgttcg ttccgtacgc cctcacgacc
     1321 gacgccggcg cgagctcggg cgtcgtgtgg caggagaggc agccggcgtc cgtgatcttg
     1381 atcgggcgcg cgaggtgcag cgaccggccg agcggaccct cgcgctcgcc gatgacctcg
     1441 gtgagcttcg ggtccgcgcg gaacgcgttc acgacgtcgg cctcccagtc gaccgtccgg
     1501 tcgcgggggt tcgtcgggtt cagcgtcgct tccttgtagg tgtactcggg gttgctctcc
     1561 cggaggttgt tgaagatctc cgtcgccgag tacgcgggga cggactgcgg cagaaactcc
     1621 tccgcgagcc gcggcgcgag gagcggcttg atctgcttgt tcgtgtagcc gcgcgtcgcg
     1681 agtgcggtct gcatcatgag ccgcgcgttc tggacgacct gcgtgcgcgc gttcgtctgc
     1741 agaagctggt tcgagaggta ggccgccggt acgagggcca cggcgaagac gaggatcagg
     1801 atcaagttga acttgacgaa cagtgacatc gtgcgcctct ttgtgcgact gattctaacc
     1861 cgcaggtatc gccccgggga ggaggccgag gccggaccgg acggtgcgcc gcttgacaaa
     1921 acgtgaaatc gaaactatct ttgttacgga atggctgcca acacccgttt cgccgtcggt
     1981 gtccacctcc tgacgtccct cgcctggagc ggaggaatcc tgacgtcgga agagctcgcc
     2041 gggagcgtcg tgacgaacgc ggtcgtcctg cgaaggatcc tgtccctcct cgagaaagcc
     2101 gggctcgtct ccgggcaccc gggccgaaag ggcgggttca cgctcgcccg gccggcctcg
     2161 gccatcacgc tcctcgacgt ccaccgcgcg gtgggcggga aggggctctt cgccgtccac
     2221 gcgaacaagg aggcgagaaa ctgccccgta agcgcctgca tcaaaggcgt gctcgccgag
     2281 gtcttcacga aggccgaggg cgccgccgag ggcgagctcc ggcgggtgcg cctctcggac
     2341 gtcgtcgccc gcgtcccctc ggccgggggg aagaaggggg cctgactttt tttgtcgaga
     2401 ttcgtaacca tattggttgc gtaaacttcg gtctttcgaa aggagacacc ccatgaagaa
     2461 caccctcgcc ctcaccgccg ccgccgccgc cttcgcggtc tccaccgccg tcgccgagcc
     2521 cgccgtcggc cccgccagcg ccgccggcgc ctggacgatc gacaaggccc actccgaggt
     2581 gggattccag gtccgccacc tcatgacgaa ggtccgcggc tcgttcggcg cgttcgacgg
     2641 ctccgtgaag atcgacgcgg caaagcccga agcgtcgacc gtgtccttcg tcgtgaaagc
     2701 cgcgagcgtg aatacgaacg agacgaagcg cgacgagcac ctgcgctccg ccgacttctt
     2761 cgacgccgcg tcgtacccgg agatccggtt cgtctcgacg tccgtgaggc ccgccggcga
     2821 gaaccgctac gaggtcaccg gcgacctcac gctgcggggc gtcacgcgga aggtgtccct
     2881 ccccgtcgtg tttctcgggg tccagaagga cccctggggc agcgagaaag ccgggttcga
     2941 gacggccgtg acgctcaacc ggaaggacta cggcatggtc tggaacaagg cgctcgacca
     3001 cggcggcacg atcctcggcg acgaggtctt cgtgacgatc gccctcgaag ccgcgcgcgc
     3061 gaaggcgaac tgaactcgcg tttcgtcctg gaggcgggcc gctcccccgt ggggcggctc
     3121 gccgcccgtc cggccgtcag ccgcggcccg agacgaagcg cgccgtgacg aggtccatcg
     3181 tctggatgtg gttcacgaac cactgcggga cgtcgccgag gacccacgcc cggaggcgcg
     3241 cggcgtcgcc cgtctcccgg aagcggcgga tctcgtcgtc gagctccgcg aggacgcgca
     3301 cgtgctcggc gtggtggacc gggaacgccg ggaatcccga acgccgcatc gcgtcgtcct
     3361 cgcgtccgaa gtgggcggcc gtgtggtcgc gcagcgccgc gaggctggcg agcgccgcgg
     3421 gcgcgtcgcc caaccccgct tcgagcgcgt ccaccgcgtc ggccaccgcg ttcagcagcc
     3481 gcccttcctc gcgatggtcc gcgttcacga agtcgagggg aacctggggg atcgcgtcga
     3541 ggtcgaccag cggcatgggc atctccagcg cctgttgtaa ccgtcccgag ggcgtcctgt
     3601 ccatccgtca cccgtagaat ccggccatgc gtccccggga ccgccattcg gcggcgccgc
     3661 ctccggcgtg aaggtcctcc gcttcctcct cgcgttcgtc cccctcgcgc tcgccgccga
     3721 gtggcttttc cattccccgg cgctcgtctt cgccttcgcg tgcctcgggc ttctcccgct
     3781 cgcgggcgcg atgggagagg cgacggagca gatcgcgcac cgcacgggcg agcgcgtggg
     3841 cggtctcctg aacgcgacgc tcggaaacgc cgccgagctc atcatcacga tcgtggcgct
     3901 ccgggccgga aaggtcgagc tcgtcaaggc ttcgatcgtc gggtcgattc tcggaaacct
     3961 cctcctgatc ctcgggctct cgctcctcgt cggtgggctg aagaacgggc tccagcgctt
     4021 cgactcgcgc gccgcgggca cggcctcgac gatgatgctg ctctccgtca tcgggctcct
     4081 gatcccgacg ctcttcgaga tcgtccacaa ggtgcagcac cacctgccga tcctctccga
     4141 gtcgccggac ccgctcctcg acaagctctc cctcggcgtc gccgcggtcc tgatgctcgt
     4201 ctacgtgctc tccctcgtct tctcactcac gacgccgtcg cgcggcatcg caccggaagt
     4261 cggcgtgacg caccgggagg cgcccgaggg cgcgtggagc ctcggcgcgg ccgtgggcgt
     4321 cctcgtcggc tcgacgctcg cgacggtcgt cctctccgag ctcctcgtcg gcgccgtcga
     4381 acccgtcgtg cgccggacgg gcgtgtcgga ggtcttcctc ggcatcgtcc tgatcccgct
     4441 cgtcggcaac gtcgccgagc acctcgtcgc cgtctcgacg gcggcgaaga accggatgga
     4501 gctgtccctc gcgatctccc tcggctcgag ccagcagatc gcgctcttcg tcgcgccgct
     4561 cctcgtcttc acgtcgcttc tcttcgggaa ggagatgacg ctcttcttca acccgttcga
     4621 ggtcgcggtc ctcggcgtct cggtcctcat cgccggccag atcgccgcgg acggcgagtc
     4681 gaactggctc gaaggcgcgc agctcctcgt tgtctatgcc atcgtcgcga tggcattctt
     4741 gtggctgtga gacgcctcgg ggccgtcctc atcgccgtcg gcgtggtcct cctcggcgcc
     4801 tgggccgcgg cgagggtgca cacggttctc gggtcgcggc aggaccggtc gcggttcgag
     4861 gagcagcgcg cggccggccc gcgcaagcgg ctcgtcgtcc tgacgttcga cgatccgcgg
     4921 gcgttcccct ggggcggcga gcccgtgctg atggacggcc gcaacgtcgg cgagatcacg
     4981 agcgccgggt acagccgc
//
LOCUS       DAOMFY010000289         4960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6016_length_4960_cov_6.011825, whole genome shotgun sequence.
ACCESSION   DAOMFY010000289 DAOMFY010000000
VERSION     DAOMFY010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6016_length_4960_cov_6.011825"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..844
                     /locus_tag="PLB01_16960"
     CDS             155..844
                     /locus_tag="PLB01_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HQQ79046.1"
                     /translation="MRDLPPSHALLARLVDCVADTTSRFFGDPAALDALAGEIAPERL
                     LALGAEGTLDIWCAGCGTGEEAWSVAIRLAERGLVSHRRVRIRGTDLSPAAIRHAKAG
                     VYGPHALRGVAEAVRTRHFQPLSNGRYRVREPLREAVYFEARSLADEPPGAGKHDAII
                     CHGLLAQLPAAARPEAVERLGASLKPGGYLLLGREDHPFAASAPLAPVLLGSDLAYRT
                     PGAVVYTRALS"
     gene            841..3408
                     /gene="leuS"
                     /locus_tag="PLB01_16965"
     CDS             841..3408
                     /gene="leuS"
                     /locus_tag="PLB01_16965"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQQ79047.1"
                     /translation="MTTPTVPPSRSDEIRRIETKWRERWEQDRAAFVDTGKPAGDGTY
                     LLVMLPYPSGDRLHVGHARTYFLTDALHRYLRQRGRTILCPMGWDAFGLPAENYAIQK
                     GIHPRTSTLANIAAMKEQFRAWGVLYDWTKEVTTCEPAYYRWNQWFFLKLLEKGLAVR
                     RKSAVNWCPSCETVLANEQAEGGVCDRCGTPVVQRELEQWFLKITDYAESLLAGLDTL
                     GNWPEKVVTMQRNWIGKSVGADLDFAIPALGESVRVFTTRPDTVFGATALILAPEHAL
                     VPRLIAGHPDEAGLKAWVESVRDQERIAREAEGAEKDGRDTGVKAINPFTGKEIPVWL
                     ANFVIADYGTGALMAVPAHDTRDFEFATKYGIPIVRVIEEERKEKKEDLPRREEGEGA
                     AAGAGAPSGGASAPAAAYTGEGHLIASGPFSAKASSEARELISADAASRGIGGPRIRY
                     RLRDWLISRQRYWGTPIPMVRCEKDGWVPVPEKDLPIVLPPDAPFTGKGGNPLEKVAS
                     FVNTSCPKCGGPARRETDTMDTFVDSSWYYLRYLDPANGNAPFDAARAAEWAPVDQYI
                     GGIEHAILHLLYARFFARVMKDLGLVAFEEPFSALFNQGMITRQSPTGRVEKMSKSRG
                     NAVSLDPLIAEKGADAVRAFVLFLGPPEKDAEWSDEGISGPERFLGRVRTVVERFVAS
                     GADVRGEPAAADTPAARARHMAVKRVTEDFDAFSFHTAVAHLMEFGTHAAALVGDAKA
                     DPGETAATLRALVALLHPIAPHLSEELNEKLGGRESLLVSGWPAFDPKLAVEEVATVA
                     VQVSGKVRAEVRLPRGSSEKEAVAAAVAEPAMARWLEGKQTVKVIWVQDRLLNLVVK"
     gene            3459..4043
                     /locus_tag="PLB01_16970"
     CDS             3459..4043
                     /locus_tag="PLB01_16970"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HQQ79048.1"
                     /translation="MRTLPEVRPRRRAPCSFSSPFREIFLFSLSLFLPVSLSGCGYAL
                     VGTGASAIPATVKTVWVPTFINDTTVVAAEQKLTDAVLRELSARGRLKPVPDRAQADA
                     ELNGRLTSLSLTPVRFDDAGLAVEYQLTITANLSLAEKSTEKILFKEPSFVFRQPYNV
                     LGSSKSYYDREREAIEALARPFAQSLVTTILEGF"
     gene            complement(4050..4355)
                     /gene="rpsT"
                     /locus_tag="PLB01_16975"
     CDS             complement(4050..4355)
                     /gene="rpsT"
                     /locus_tag="PLB01_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQQ79049.1"
                     /translation="MANTKQAKKRAGQSEKRRARNRSVVTRVRNVVKNLRKTAETEAA
                     KVGGMLSNAMSELDVAVRKGVLHKKTASRLKSRIAKTTNKAAKAAGAPAAKAAKKAK"
     gene            complement(4386..>4960)
                     /gene="ybgF"
                     /locus_tag="PLB01_16980"
     CDS             complement(4386..>4960)
                     /gene="ybgF"
                     /locus_tag="PLB01_16980"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQQ79050.1"
                     /translation="PATAASRMAGRSGSAAAIPVLAPPGPGGAAATPSASSPASGGLV
                     AVPAAAAAAAATAPKGGGISPADLFAQATADYQRGRYDLARQGFEDYAEKFPRTDLSD
                     DALYWAGECWSAQKKPHEAITAYDKLFRTYPQSDKAPAAHLKKGLAHLDLGEKAQAMV
                     ELNYVVNQFPGSDEAKSARQRLKALGGDAR"
BASE COUNT          716 a         1724 c         1753 g          767 t
ORIGIN      
        1 gatctgctga gcgcgggcca ggtagaagcc gccggagcgg tagtgcagca gcgccgggtc
       61 ggcgctcgac gccgaggccc tgcggccgag gctcgggttg ctcctcgccg ccgaagcctg
      121 ggccgcgggt tcgttccggc gcctctggcg ccgcatgcgc gacctgccgc cttcccacgc
      181 cctgctcgcg cggctcgtgg actgtgtggc ggatacgacg tcgcggttct tcggcgaccc
      241 ggcggcgctc gacgcgctcg cgggagagat cgccccggag cggcttctgg cccttggagc
      301 cgaaggcacg ctcgacatct ggtgcgcggg atgcggcacg ggcgaggaag cctggtcggt
      361 cgcgatccgg ctcgccgagc gcgggctcgt ctcgcaccgg cgggtccgga tccggggcac
      421 ggacctgtcg ccggccgcga tccggcacgc gaaggcgggc gtctacggcc cccacgcgct
      481 gcgcggcgtc gcggaggcgg tgcggacgcg ccacttccag ccgctgtcga acggccgcta
      541 tcgcgtgcgc gagcccctcc gcgaggccgt ctacttcgag gcacgctctc tcgcggacga
      601 gccgccgggc gcggggaagc acgacgcgat catctgccac ggcctcctcg cgcagctgcc
      661 ggcggcggcc cgccccgagg cggtcgagcg cctcggcgcg tccctgaagc ccgggggcta
      721 cctgctgctc gggcgagagg accacccgtt cgccgcctcc gcgccgctcg cgcccgtcct
      781 cctcgggagc gacctcgcct accggacgcc cggcgccgtc gtttacactc gcgcgctctc
      841 atgacgacgc cgaccgttcc cccctcgcgc agcgacgaaa tcagacggat cgaaaccaag
      901 tggcgcgagc gctgggagca ggatcgcgcc gccttcgtgg acacgggcaa gcccgcgggc
      961 gacggcacgt acctcctcgt gatgctgccc tacccctcgg gcgaccgcct gcacgtcggg
     1021 cacgcgcgga cgtacttcct gacggacgcg ctgcaccgct acctgcgcca gcggggcagg
     1081 acgatcctgt gcccgatggg ctgggacgcc ttcgggctcc cggccgagaa ctacgcgatc
     1141 cagaagggca tccatccccg cacctcgacg ctggcgaaca tcgcggcgat gaaggagcag
     1201 ttccgggcgt ggggcgtcct ctacgactgg acgaaggaag tcacgacctg cgagccggcc
     1261 tactaccgct ggaaccagtg gttcttcctc aagctcctcg aaaagggcct cgccgtgcgg
     1321 cggaaatccg ccgtgaactg gtgtccctcg tgcgagaccg tgctcgcgaa cgagcaggcc
     1381 gagggcggcg tgtgcgaccg ctgcgggacg cccgtcgtcc agcgcgagct cgagcagtgg
     1441 ttcctgaaga tcacggacta cgcggagagc ctcctggcgg gcctcgacac gctcgggaat
     1501 tggcccgaga aggtcgtcac gatgcagcgc aactggatcg ggaagtcggt cggcgccgac
     1561 ctcgacttcg cgatccccgc cctcggcgag agcgtgcgcg tcttcacgac gcgcccggac
     1621 accgtcttcg gcgcgaccgc gctcatcctc gcgcccgagc acgcgctcgt cccgcgcctg
     1681 atcgcgggcc atccggacga ggcgggcctc aaggcgtggg tcgagtccgt tcgcgaccag
     1741 gagcgcatcg cgcgcgaggc cgagggcgcc gagaaggacg gccgcgacac gggcgtcaag
     1801 gcgatcaacc cgttcaccgg gaaggaaatc cccgtgtggc tcgcgaactt cgtcatcgcg
     1861 gactacggca ccggcgcgct catggccgtc cccgcgcacg acacgcgcga cttcgagttc
     1921 gcgacgaagt acgggattcc gattgttcga gtcattgagg aagaaagaaa ggaaaagaaa
     1981 gaagatttgc ctagaaggga agagggggag ggcgccgcgg cgggtgccgg agccccttct
     2041 ggcggcgcca gcgcaccggc cgccgcctat acgggcgaag gccacctcat tgcctctggc
     2101 ccattctcag caaaagcttc ttccgaagcc cgcgaactga tttcggcgga tgccgcgtcg
     2161 cgaggaatcg gcggcccccg catccgctat cgcctgcgcg actggctcat ctcccgccag
     2221 cgctactggg gaactccgat cccgatggtc cggtgcgaga aggacgggtg ggtgcccgtc
     2281 cccgagaagg acctgccgat cgtgcttccg cccgacgccc cgttcacggg caagggggga
     2341 aacccgctcg agaaggtcgc ctcgttcgtg aacacttcgt gcccgaagtg cggcggcccg
     2401 gcgcgccgcg agacggacac gatggacacg ttcgtggact cgtcctggta ttacctgagg
     2461 tacctcgacc ccgcgaacgg gaacgcgccc ttcgacgcgg cccgcgccgc ggagtgggcg
     2521 cccgtcgacc agtacatcgg cgggatcgag cacgcgatcc tccacctcct ctacgcgcgc
     2581 ttcttcgcgc gcgtgatgaa ggacctcggc ctcgtcgcgt tcgaggagcc gttctcggcg
     2641 ctgttcaacc agggaatgat cacgcgccag tcgccgaccg ggcgcgtcga gaagatgtcg
     2701 aagtcgcgcg gcaacgccgt gtctctcgat ccgctcatcg cggagaaggg cgccgatgcc
     2761 gtgcgcgcgt tcgtcctctt cctcgggccg cccgagaagg acgcggagtg gagcgacgag
     2821 ggcatcagcg ggccggagcg tttcctcggg cgcgtccgga cggtcgtcga gcgcttcgtc
     2881 gcgtccggcg ccgacgtgcg cggcgagccc gcggccgccg acacgccggc ggcgcgcgcc
     2941 cggcacatgg ccgtcaagag ggtcacggag gatttcgacg cgttctcctt ccacacggcc
     3001 gtcgcgcacc tcatggagtt cggcacgcac gcggccgccc tcgtcggcga cgcgaaggcc
     3061 gatccggggg agaccgccgc gacgctccgc gcgctcgtcg cgctcctgca tcccattgcg
     3121 ccgcacctca gcgaggagct gaacgagaag ctcggcggca gagagagcct cctcgtctcc
     3181 ggctggccgg ccttcgaccc gaagctcgcg gtggaagaag tcgcgacggt cgccgtgcag
     3241 gtttcgggaa aggtccgcgc ggaggtcagg ctgccgagag ggtcttccga gaaagaagcg
     3301 gtcgcggccg ccgtggcaga gccggccatg gcccgctggc tggaaggcaa gcagaccgtc
     3361 aaggtcatct gggtccagga ccgcctgctg aacctggtcg tgaagtgaga gagaaggaaa
     3421 ggacagaaga ccgaagaggg gattcttcga atggtcgcgt gcgaaccctg cccgaggtgc
     3481 gcccgcggcg ccgcgctcct tgctctttct cttcaccttt cagagaaatc ttcctcttct
     3541 ctctctccct ttttctccct gtctcgcttt cgggctgcgg ctacgcgctc gtgggcaccg
     3601 gcgcgagcgc gatcccggcg accgtcaaga cggtctgggt gccgacgttc atcaacgaca
     3661 cgacggtcgt ggcggccgag cagaagctca cggacgcggt cctccgggag ctctcggcgc
     3721 gcggacgcct gaaaccggtc ccggatcgcg cccaggcgga cgcggagctg aacgggcgcc
     3781 tgacgtctct gagcctcacg ccggtccggt tcgacgacgc cgggctcgcg gtcgagtacc
     3841 agctgacgat cacggcgaac ctctccctcg ccgagaagtc gacggagaag atcctgttca
     3901 aggagccgtc gttcgtgttc cgccagccct acaacgtgct ggggagctcg aagtcgtact
     3961 acgaccgcga gcgggaagcg atcgaggccc tggcgcgacc gttcgcgcag agcctcgtca
     4021 cgaccatcct ggaagggttc taggggcccc tacttcgcct tcttggcggc tttcgcggcg
     4081 ggcgctccgg cggctttcgc ggccttgttc gtcgtcttcg cgatgcgcga cttcaggcgc
     4141 gaggcggtct tcttgtggag gacgcccttg cggacggcga cgtccagctc ggacatcgcg
     4201 ttcgagagca tgccgccgac cttggccgcc tcggtctcgg cggtcttgcg gaggttcttg
     4261 acgacgttgc ggacgcgcgt gacgacggag cggttgcggg cgcggcgctt ctcgctctgt
     4321 ccggcgcgct tcttggcctg cttggtgttt gccattgcgg ggaaggtccc tttctttcgg
     4381 ttgtcttacc gggcgtcgcc gccgagagcc ttgagccgct ggcgcgcgct cttggcctcg
     4441 tcggatcccg ggaactggtt gacgacgtag ttgagctcga ccatcgcctg ggccttctcg
     4501 ccgaggtcga ggtgggcaag ccccttcttc aggtgggcgg cgggcgcctt gtcgctctgc
     4561 ggatacgtgc ggaagagctt gtcgtaggcc gtgatcgcct cgtgcggctt cttctgcgcc
     4621 gaccagcact cgcccgccca gtacagggcg tcgtccgaga ggtccgtgcg cgggaacttc
     4681 tcggcatagt cctcgaagcc ctgtcgcgca aggtcgtagc ggccgcgctg gtagtccgca
     4741 gttgcctgcg cgaagaggtc ggcgggggaa atgcccccgc ccttcggcgc ggtggccgcg
     4801 gcggctgcgg cagcggcagg cacggcgacg aggccgccgg aagcgggaga ggatgcgctc
     4861 ggcgtcgccg ccgcgccacc cggcccgggc ggggcgagta cggggatggc ggccgccgag
     4921 ccggaccggc cggccatcct gctcgccgcc gtggcggggc
//
LOCUS       DAOMFY010000290         4933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6062_length_4933_cov_7.699057, whole genome shotgun sequence.
ACCESSION   DAOMFY010000290 DAOMFY010000000
VERSION     DAOMFY010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6062_length_4933_cov_7.699057"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..911)
                     /locus_tag="PLB01_16985"
     CDS             complement(114..911)
                     /locus_tag="PLB01_16985"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ79051.1"
                     /translation="METGATAATPERLQAHMRAVARQQYDSVPVPPFTLYFHPSDALP
                     YLNYAIPDEEPGNAASLAAPLAELKAEFARRGRRPRFEFVGAVFPALAGALEASGFTI
                     EARPQLMVATPDSLRDVPPAAGLDVETVSATSPLDAFRVFISVQRRAFGLGDAAAVSD
                     QDALWLRDGLGRGLCFVGRLAGEAVAVSMFLDPQDGLTELAGICTLEAHRRRGLAGAL
                     THAALREAFRRGVSVAFLSAADARAGRVYEAAGFAPFGNTVFAIAKT"
     gene            984..2291
                     /locus_tag="PLB01_16990"
     CDS             984..2291
                     /locus_tag="PLB01_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ79052.1"
                     /translation="MTLHRIPRLAPAAALLAAFALLAETLAPKNPSAKKVVLVKAGAL
                     VDVLSGRVLANQDVLIEDGVVKQVGPGIAAPAGASVVDLTKKTVLPGLIDVHTHITSQ
                     PEDYYADRFRKSPIDVAVTAHVYAKRTLEAGFTTLRVVGSPEYVDVALKSAIDKGQVA
                     GPRLKVAGLSIGSTGGHGDLTGFSPYLKFERFSGVADGVDAVRRLVRQNVKFGADVIK
                     MIASAGVLSEEESVGAPQYSPEEMKAICDEAAMWGRKVAAHAHGTEAIKRAVAAGVAS
                     VEHGSLIDDEGIRMMKERGTFLVADVYNDDWILAEFAKMGYPEKIIEKERSVGRLQRE
                     NFAKAVKAGVKVAYGTDAGVYPHGLNARQFRYMVAGGLTPMQAIQSATANAAELLGWE
                     KVGAIAPGRFADLVAVDGDPLKDATVLERVSFVMKGGEVVVSR"
     gene            2288..3970
                     /locus_tag="PLB01_16995"
     CDS             2288..3970
                     /locus_tag="PLB01_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQQ79053.1"
                     /translation="MSRIAPLAVLLALSGRAFAADPVPTPLPVPAPDAGTVFRGASVY
                     TAWDETPRKLAILVKDGKVVSVGYEAEVRAAAREAKVVDLAGTVLVPGLTDAHGHLRS
                     LGALHRMIDCRGLTKEEILLRVRALSASTAPGAWIRGRAWDQNRWTDTAFPTAADLEP
                     AAPKTPVVLGRVDGHAIWVNAAALKAAGITKATPDPPGGRIERLKDGSPAGVLVDNAM
                     SLVWKAIPPPGPEEARKDFVAGLEACAKAGLTGIGEASGSSLADLAILEGLAKEGKLP
                     VRVYATVGPKELDAALARGPVASGRFTLRAVKLYADGALGSRGAALLADYSDAPGNRG
                     LDVTPPETVAALAEKAFRGGFQVWTHAIGDRANRTTLDAYEKGLEAAKPADARPRIEH
                     AQVVAPGDAERFARLGVIASVEPTHATSDWIWAEGRLGKERVKGAYAWRTLLKAGAKL
                     AGGSDFPVESEDPLLGIFAAVTRQETNGKPPGGWRPEEGLGRAEALRLFTSDAAYAEF
                     AEKRRGRIEPGFDADFTVLDREIFAPGTPAGEILKAKVVMTVVGGEIVYRAN"
     gene            complement(3967..4167)
                     /locus_tag="PLB01_17000"
     CDS             complement(3967..4167)
                     /locus_tag="PLB01_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79054.1"
                     /translation="MNGDRKLFTCGKCGKKFWYWVLDDVAQPKIKCYFCSTEAYPNGE
                     PPKPPAPAPAPPAPAPPAAPPA"
     gene            complement(4178..>4933)
                     /locus_tag="PLB01_17005"
     CDS             complement(4178..>4933)
                     /locus_tag="PLB01_17005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQQ79055.1"
                     /translation="RRLGARCIRVPVRPRDGQQRLDRTGREFQRPLGLAARGLAAGVF
                     RPGTPGWTAFAPTPPPANPSAAPASIAFFAVQALVEELLFRGALMTLIAALALAVLRL
                     ARGPLPGRGDAAGLTRFGRRWLAAGLAANVAQAAGFAALHADNPHTTPLALVNIGLAG
                     AVLGWLFWSGGGLAGAWAFHALWNFSLSALGLPVSGLALETPLVATGFAGAGRPLLSG
                     GAFGPEGSLVNTAALALALAFLIRRSARRLPNG"
BASE COUNT          729 a         1731 c         1871 g          602 t
ORIGIN      
        1 cacgctcgca gccgaccccg gcgcgctcgc ccgctggacc gaggtcgccc gcctccccgc
       61 gaatgcgttt gggtctgccg gcgccgtgcg cgtctaccgc cggttacgct gagtcaggtc
      121 ttcgcgatcg cgaagaccgt gttcccgaac ggcgcgaagc cggcggcctc gtagacgcgg
      181 ccggcgcgcg cgtcggccgc cgagaggaac gcgacggaga cgccgcgccg gaaggcctcg
      241 cggagggccg catgcgtgag cgcccccgcg aggccgcggc ggcggtgggc ttcgagcgtg
      301 cagatcccgg cgagctcggt gaggccgtcc tggggatcga ggaacatcga gaccgccacg
      361 gcctcgccgg cgaggcgccc cacgaagcac agcccccggc cgaggccgtc gcgcagccag
      421 agcgcgtcct ggtcggagac cgcggccgcg tcgccgaggc cgaacgcacg ccgctggacg
      481 gagatgaaga cgcggaaggc gtcgagcggc gacgtcgccg aaaccgtctc gacgtcgagc
      541 ccggcggccg gaggaacgtc gcggagcgag tcgggcgtcg cgaccatgag ctgcgggcgg
      601 gcctcgatcg tgaagccgga ggcctcgagg gcgccggcga gcgcggggaa gaccgcgccg
      661 acgaactcga agcgcggcct gcgcccccgc cgcgcgaact cggccttcag ctccgcgagc
      721 ggcgccgcga gcgaggccgc gttgcccggc tcttcgtcgg ggattgcgta gttgaggtag
      781 ggcagcgcgt cggacggatg gaagtagagc gtgaacggcg gtaccgggac ggagtcgtac
      841 tgctgccgtg cgacggcgcg catgtgcgcc tgcaggcgtt cgggtgtcgc cgcggtcgcg
      901 ccggtctcca cgccgccatt ctagggacac tcgtcctcgg gccgcccggc tggtaaggtt
      961 cgcgcgtccc acaaggaggc cccgtgacgc tgcaccgaat cccgagactc gcccccgccg
     1021 ccgcgctgct cgcggcgttc gccctgctcg ccgagacgct ggccccgaag aatccttccg
     1081 cgaagaaggt cgtcctcgtg aaggcgggcg ccctcgtcga cgtcctctcg ggacgcgtcc
     1141 tcgcgaacca ggacgtcctg atcgaggacg gcgtcgtgaa gcaggtgggc cccgggatcg
     1201 cggcccccgc gggcgcgtcc gtcgtggacc tgacgaagaa gacggtcctg ccgggcctca
     1261 tcgacgtcca cacgcacatc acgtcgcagc ccgaggacta ctacgccgac cgcttccgca
     1321 agtcgccgat cgacgtcgcc gtcacggcgc acgtctacgc gaagcggacg ctcgaggcgg
     1381 gcttcacgac gctgcgggtc gtcggcagcc cggagtacgt cgacgtcgcg ctcaagagcg
     1441 cgatcgacaa gggacaggtc gccgggccgc gcctgaaggt cgccggcctc tcgatcgggt
     1501 cgaccggcgg ccacggggac ctcacgggct tctcgccgta cctgaagttc gagcgcttct
     1561 cgggcgtcgc cgacggcgtc gacgccgtcc gcaggctcgt ccgccagaac gtgaagttcg
     1621 gagcggacgt catcaagatg atcgcctcgg ccggcgtcct ctccgaggag gagtcggtcg
     1681 gggcgccgca gtactccccc gaggagatga aggcgatctg cgacgaggcc gcgatgtggg
     1741 gccgcaaagt cgccgcgcac gcgcacggaa ccgaggcgat caagcgcgcc gtcgccgcgg
     1801 gggtcgcgtc ggtcgagcac gggagcctga tcgacgacga gggcatccgg atgatgaagg
     1861 agcggggcac gttcctcgtc gcggacgtct acaacgacga ctggatcctc gcggagttcg
     1921 cgaagatggg ctatcccgag aagatcatcg agaaggagcg gtcggtcggg cgcctccagc
     1981 gcgagaactt cgcgaaggcc gttaaggcgg gcgtgaaggt cgcgtacggg acggacgcgg
     2041 gcgtctaccc gcacggcctg aacgcgcggc agttccggta catggtcgcg ggcggcctca
     2101 ctccgatgca ggccatccag tccgccacgg cgaacgcggc cgagctgctc ggctgggaga
     2161 aggtcggcgc catcgcgccc ggccgtttcg cggacctcgt cgcggtggac ggcgatccgc
     2221 tcaaggacgc gactgttctc gagcgcgtgt cgttcgtgat gaagggcgga gaggtcgtgg
     2281 tgtcgcgatg agccggatcg ccccgctcgc cgttctgctc gcgctttccg gccgcgcgtt
     2341 cgccgccgac cccgttccca cgccgctccc cgttccggct ccggacgccg ggacggtgtt
     2401 ccgcggggcg tcggtctaca cggcgtggga cgagacgccg cgcaagctcg cgatcctcgt
     2461 caaggacggg aaggtcgtct ccgtcgggta cgaggccgag gtccgtgccg ccgcgcgcga
     2521 ggcgaaggtc gtcgatctcg cgggcaccgt cctcgtcccc gggctcacgg acgcccacgg
     2581 ccacctgcgc tccctcggcg cgctccaccg gatgatcgac tgccgcggcc tcacgaagga
     2641 ggagatcctc ctgcgcgtcc gggctctctc ggcctcgacg gcacccgggg cgtggattcg
     2701 cggccgcgcg tgggaccaga accgctggac ggacacggcg ttccccaccg cggccgacct
     2761 cgagccggcc gcaccgaaga cgcccgtcgt cctcgggcgc gtggacggac acgcgatctg
     2821 ggtgaacgcc gccgcgctga aggccgcggg catcacgaag gcgacgccgg acccgccggg
     2881 tggacgcatc gaacgcctca aggacgggtc gcccgcgggc gtcctcgtcg acaacgcgat
     2941 gagcctcgtc tggaaggcga ttccgccgcc cggtcccgag gaggcgcgca aggacttcgt
     3001 ggccggactc gaagcctgtg cgaaggccgg gctcacgggc atcggcgaag cgtcgggctc
     3061 gtcgctggcc gacctcgcga tcctcgaggg gctcgcgaag gaaggaaagc tgcccgtccg
     3121 cgtctacgcg accgtcggac cgaaggaact cgacgcggcg ctcgctcgcg ggcccgtcgc
     3181 gtccggccgc ttcacgctcc gggcggtgaa gctctacgcg gacggcgccc tcggttcgcg
     3241 cggggccgcg ctcctcgcgg actattcgga cgcgccgggc aaccgcggtc tggacgtcac
     3301 gccgcccgag acggttgcgg cgctcgcgga gaaggcgttc cgcggaggct tccaggtgtg
     3361 gacgcacgcg atcggggacc gcgcgaaccg cacgacgctc gacgcctacg agaaaggcct
     3421 cgaggcggcg aagcccgccg acgcgcgccc gcggatcgag cacgcccagg tcgtcgcgcc
     3481 gggcgacgcc gagcgcttcg cgaggctcgg cgtcatcgcg tccgtcgagc ccacgcacgc
     3541 gacgtccgac tggatctggg ccgagggccg gctcgggaag gagcgcgtga agggcgcgta
     3601 cgcgtggcgc acgctcctga aggcgggggc gaagctcgcg ggaggcagcg acttcccggt
     3661 cgagtcggaa gacccgctcc tcgggatctt cgcggcggtc acgcggcagg agacgaacgg
     3721 gaaaccgccc ggcggctggc gcccggagga gggactcggg cgcgcggagg cgctgcgcct
     3781 cttcacgtcc gacgccgcgt acgccgagtt cgccgaaaag cgccgcgggc ggatcgagcc
     3841 cggcttcgac gccgacttca cggtgctcga ccgcgagatc ttcgcgccgg ggacgcccgc
     3901 cggcgagatc ctcaaggcga aggtcgtgat gaccgtcgtc ggcggggaga tcgtctatcg
     3961 ggcgaactag gccggcgggg ccgcgggcgg ggccggtgcc ggaggcgccg gggccggagc
     4021 cggcggcttc ggcggctctc cgttcgggta cgcctcggtc gagcagaaat agcacttgat
     4081 cttcggctgc gcgacgtcgt cgagcaccca gtaccagaac ttcttgccgc actttccgca
     4141 ggtgaagagc ttgcgatcgc cgttcatggt tccgatccta cccgttcggc agacgccgcg
     4201 cgctgcgccg gatgaggaag gcgagggcga gggcgagcgc ggcggtgttc acgagcgagc
     4261 cttccggccc gaatgcgccg cccgagagga gcggccgccc cgcaccggcg aagccggtcg
     4321 cgacgagcgg cgtctccagc gcaagcccgc tgaccgggag accgagcgcc gacagcgaga
     4381 agttccagag cgcgtggaag gcccacgcgc ccgcgaggcc gccgcccgac cagaacagcc
     4441 agcccagaac ggcgccggcg aggccgatgt tcacgagggc gagcggcgtc gtgtgcgggt
     4501 tgtcggcgtg gagagcggcg aagccggcgg cctgtgccac gttcgcagcg aggcccgcgg
     4561 cgagccagcg ccggccgaag cgggtgagcc ctgcggcgtc gccgcggccg gggagcggac
     4621 cccgtgccag gcgcaacacg gcgagcgcaa gcgccgcaat gagcgtcatc agcgccccgc
     4681 ggaagaggag ctcctcgacg agagcctgga cggcgaagaa cgcaatcgag gcgggagcgg
     4741 cggacgggtt cgcgggcggc ggcgtcggcg cgaacgccgt ccagccgggc gtcccggggc
     4801 ggaacacgcc cgccgcgagc ccgcgcgcag ccaggccgag cgggcgttga aactcgcgcc
     4861 cggttcggtc gagacgctgc tggccgtcgc gcgggcgaac cggaacacgg atgcaccgcg
     4921 cgccgaggcg gcg
//
LOCUS       DAOMFY010000291         4898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6125_length_4898_cov_7.341937, whole genome shotgun sequence.
ACCESSION   DAOMFY010000291 DAOMFY010000000
VERSION     DAOMFY010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6125_length_4898_cov_7.341937"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLB01_17010"
     CDS             <1..430
                     /locus_tag="PLB01_17010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQQ79056.1"
                     /translation="VVAAPPKQEPAAAAPIPTEVSVPEKAPVIPAATNAPVKEGELVG
                     PGEGVVEPKLVRLGPMSGMPMQARQVKRTLDGSIGTPVLMALVDEKGTVVEIRLIRPS
                     SYKFADDAAARALKGATIEPATKDGVKVKMWKTFPIAVRP"
     gene            453..674
                     /locus_tag="PLB01_17015"
     CDS             453..674
                     /locus_tag="PLB01_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79057.1"
                     /translation="MSLSEEQKKFYEHTRSVTKAEIDQLEGDIQAELSKVKERLAELQ
                     NAQKAARQMYAAACLRLGIPNDLEEAEES"
     gene            692..1492
                     /locus_tag="PLB01_17020"
     CDS             692..1492
                     /locus_tag="PLB01_17020"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HQQ79058.1"
                     /translation="MAAVLAAASPSPTPPRRIVALAPSAAEILFALGAAPRVVGVSDF
                     ARDLPGAAGKTRLGGFAPDLERVVALAPDLVVVSRDGTDRAAWEKLTAMGLTVLVTSG
                     TSLDGVLDDVVHVGAAIGEPGRAASLVRALKERIAAAEAKGRAHRGKTALVLVWPDPP
                     VVAGRATFVGDVLARAGFTNAVPASAGDWPRVSLETLAAWNPDLVVRPATAENAAAFG
                     AALQGGAFRVVPAVRDGRVATLPGDLLERPGPRLVDALEALAALGSAP"
     gene            1489..2499
                     /locus_tag="PLB01_17025"
     CDS             1489..2499
                     /locus_tag="PLB01_17025"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQQ79059.1"
                     /translation="MRPAPSGRSAWVSASVLLAALLALVGASLLFGSVRLSPGEVWNA
                     LFGGGDAQAVTIVRELRLPRALLGALVGGALAVSGAALQALLGNPLADPYLLGVSGGA
                     ACGTLLGALLGLGARGAWGSSFALPLAGFLGALVAILVVGAGASVGGRLDRGRLLLSG
                     VVVNALASAVLLFLLSFGDAARTQSFVFWMLGSLAGATPGAVKALAVYAALGLALLLP
                     LARAFDALTLGEETAATLGIPVETVKRIAYLAASLLAAAAVATTGIIGFVGLLVPHAV
                     RRLAPGHRALLVLSFLGGAALLVAADALSRSLFAPAEISVGAVTALLGAPAFLVLLRA
                     RP"
     gene            2496..3281
                     /locus_tag="PLB01_17030"
     CDS             2496..3281
                     /locus_tag="PLB01_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ79060.1"
                     /translation="MTPRLEARSLSAGYGGKAVLEGVSLGLAPGRVSVLLGENGSGKS
                     TLLKALAGVLKPRSGEVRLAGRSLGSVPRREAARAIGYLPQGFEPLFPMKARDLVLLG
                     RTPHLGAFGVPGAGDRAAAEAALLEMDAAALGDADIRAMSGGERQRVLLARVLAGTPS
                     VLLLDEPTANLDPRHRFLVLDAMRRRAAEGASVFFSTHEIDVASDGADDAVLLAGGRV
                     LAEGPVATTLTGRLLSELFAVSARVAPGEGGKPLVSFGPPPAR"
     gene            3297..4142
                     /gene="accD"
                     /locus_tag="PLB01_17035"
     CDS             3297..4142
                     /gene="accD"
                     /locus_tag="PLB01_17035"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQQ79061.1"
                     /translation="MWFRKDKGPRAPRDPKTAASLGEGLFLKCEGCRETLYLKELERN
                     VRVCPKCGFHFRMPAAERIRLLLGDDAQPLFEGVRPVDPLKFKDGKRYRDRLRDTEAK
                     TGRPDAIQAAQGEIDGVPAVVAVLDYAFMGGSMGSVVGEVLTRCAEHALKERKAYVIV
                     SASGGARMQEGILSLMQMGKVSAALARLSAARIPYVSVLTDPTTGGVTASFAMLGDVN
                     MAEPKALIGFAGPRVIEQTIRQTLPEGFQRSEFLQDHGFVDLVVPRPEMRATLARVLR
                     LLQTA"
     gene            4202..>4898
                     /locus_tag="PLB01_17040"
     CDS             4202..>4898
                     /locus_tag="PLB01_17040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQQ79062.1"
                     /translation="MSAVVASPGLAWLDALGPQRIRPGLSRTLSLLASLGNPQSSFSS
                     ILIGGTNGKGSTAATISAILTAADVPCGLYTSPHLVSVTERVRLHDRDVSVAALDAAL
                     SLVAAVSAPGDRSPTYFEAMTVAAFELFRRARVAVAVVEVGIGGRLDATNVLDPEVSV
                     VTNVAADHLDVLGPALEDVAREKAGIFRRGQPALVGAEGTEERPRAVLHAEARRVGAR
                     LVEIPHAPNWEGLT"
BASE COUNT          582 a         1810 c         1748 g          758 t
ORIGIN      
        1 ggtcgttgcg gcgccgccga agcaggagcc cgcggctgcc gctccgatac cgacggaagt
       61 gtcggttccc gagaaggcgc cggtgattcc ggccgcgacg aacgcgcccg tcaaggaggg
      121 cgaactcgtc gggcccggcg agggcgtcgt cgagccgaag ctcgtgcgtc tggggccgat
      181 gagcgggatg ccgatgcagg cccggcaggt caagaggact ctcgacggat ccatcgggac
      241 gccggtcctc atggcgctcg tcgacgagaa gggcaccgtc gtcgagatcc gcctgatccg
      301 gccctcgagc tacaagtttg ccgacgacgc ggccgcgcgc gccctgaagg gtgcgacaat
      361 cgagccggcg accaaggacg gcgtgaaggt caagatgtgg aagaccttcc cgatcgccgt
      421 tcgtccgtga ccgttcgccc gaggagtagc gcatgagcct ttccgaagaa cagaagaagt
      481 tctacgagca cacgcggtcg gtcacgaagg ccgagatcga tcagctcgag ggcgacatcc
      541 aggccgagct ctccaaggtc aaggaacggc tggccgagct gcagaacgcc cagaaggccg
      601 cgcgccagat gtacgcagcc gcgtgcctca gactgggcat cccgaacgac ctcgaggaag
      661 ccgaggaatc ctgagcctcc ggctcctcct cttggcggcc gtccttgccg ccgcctctcc
      721 cagtcccacg ccgccccggc gcatcgtcgc cctcgcccct tcggcggcgg agatcctctt
      781 cgctctcggc gcggcgccgc gcgtcgtggg cgtcagcgac ttcgcccgcg atctgcccgg
      841 cgcggcgggc aagacgcggc tcggcggctt cgcgccggac ctcgagcgcg tcgtcgcgct
      901 tgcgcccgac ctcgtcgtcg tgtcgcggga cgggaccgac cgcgcggcct gggagaaact
      961 cacggcgatg ggcctcaccg tgctcgtgac ctccgggacc tcgctcgacg gcgtgctcga
     1021 cgacgtcgtg cacgtcggcg cggcgatcgg agagccgggc agggccgcct cgctcgtcag
     1081 ggcgctgaag gagcggatcg cggcggccga ggcgaagggg cgcgcgcacc gcgggaagac
     1141 ggcgctcgtc ctcgtgtggc cggacccgcc ggtcgtcgcg ggccgggcga cgttcgtcgg
     1201 cgacgtgctc gcgcgtgccg ggttcacgaa cgccgtcccg gcttccgccg gcgactggcc
     1261 gcgcgtctcg ctcgaaacgc tcgccgcgtg gaacccggat ctcgtggtcc ggcccgccac
     1321 cgccgagaac gccgcggcgt tcggcgcggc gcttcaaggc ggcgcgttcc gcgtcgtgcc
     1381 cgcggtgagg gacgggcggg tcgcgactct gcccggcgac ctcctcgaac ggccggggcc
     1441 gcggctcgtc gacgcgctcg aagcgctggc cgcgctgggg agcgcgccgt gaggccggcc
     1501 ccgtccggcc gctccgcgtg ggtgtcggcg tcggtccttc tcgcggccct ccttgccctc
     1561 gtcggcgctt ctctcctctt cggaagcgtg cggctctcgc ccggagaggt ctggaacgcg
     1621 ctcttcggcg gcggcgacgc gcaggccgtg acgatcgtgc gcgagctgcg gcttccacgc
     1681 gcgctcctcg gcgcgctcgt gggcggcgcg ctcgcggttt cgggcgcggc ccttcaggcg
     1741 ctcctcggga acccgctggc cgacccctac ctcctcggcg tctcgggcgg cgcggcttgc
     1801 ggcactctcc tcggcgcgct cctcggcctc ggcgcgcgcg gcgcgtgggg aagctcgttc
     1861 gcgctgccgc tcgcgggctt cctcggcgcg ctcgtggcga tcctcgtcgt gggagccggc
     1921 gcatccgtcg gaggacgtct ggaccggggc cgtctcctcc tctccggcgt cgtcgtgaac
     1981 gcgctcgcgt ccgcggtcct cctcttcctc ctgtcgttcg gcgacgcggc gcgcacgcag
     2041 tcgttcgtgt tctggatgct cggctcgctc gcgggcgcca cgcccggcgc cgtcaaggcg
     2101 ctcgccgtct atgcggcgct cgggctcgcc ctcctcctgc cgctcgcccg cgcgttcgac
     2161 gcgctcacgc tcggcgagga gacggccgcg acgctcggca tcccggtcga gaccgtgaag
     2221 cgcatcgcct acctcgcagc ctccctcctc gccgccgccg cggtcgcgac gacggggatc
     2281 ataggcttcg tcggcctcct cgtccctcac gccgtgcgcc ggctcgcgcc cgggcaccgc
     2341 gccctcctcg tcctgtcgtt ccttggaggc gcggcgctcc tcgtcgcggc cgacgccctc
     2401 tcgcggtccc tgttcgcgcc ggccgagatt tccgtcggcg ccgtcacggc cctcctcggc
     2461 gcgcccgcgt tcctcgtcct cctgagggcc cgcccgtgac gccgcggctc gaggcgcgct
     2521 ccctttcggc gggctacggg gggaaggccg tcctcgaggg tgtctcgctc ggcctcgcgc
     2581 ccggccgcgt gtcggtcctc ctcggagaaa acggctcggg caagtcgact ctcctgaagg
     2641 ccctcgcggg cgtgctgaag ccgcggtcgg gcgaggtgcg gctcgcgggg cggagcctcg
     2701 ggtccgttcc gcgccgggaa gccgcgcgcg caatcggcta ccttccgcag ggtttcgagc
     2761 cgctcttccc gatgaaggcg cgggatctcg tccttctcgg ccgcaccccg cacctcggag
     2821 ccttcggcgt tccaggcgcg ggcgaccgtg ccgcggcgga ggccgcgctc ctcgagatgg
     2881 acgcggcggc gctcggggac gcggacatcc gcgccatgtc gggcggcgag cgccagcgcg
     2941 tgctcctcgc ccgcgtcctc gcgggaaccc cgtccgtcct ccttctcgac gagccgacgg
     3001 cgaacctcga cccgcggcac cggttcctcg tcctcgacgc gatgcgccgc cgcgccgccg
     3061 aaggcgcgag cgtcttcttc tcgacacacg agatcgacgt cgcctcggac ggagcggacg
     3121 acgcggtcct cctcgccggg gggcgcgtcc tcgcggaggg ccccgtcgcg acgacgctca
     3181 cgggccggct gctctcggag ctctttgcgg tctcggcgcg cgtcgctccc ggggagggcg
     3241 gaaagcctct cgtctcgttc ggcccgcctc cggcgcggta acatccccgt ttgcccatgt
     3301 ggtttcgcaa ggacaagggc ccgcgcgccc cgcgtgaccc caagaccgcg gcctcgctcg
     3361 gcgaggggct gttcctcaag tgcgagggct gccgcgagac gctctatctc aaggagctgg
     3421 agcgaaacgt gcgcgtctgc ccgaagtgcg ggttccactt ccggatgccg gcagccgagc
     3481 ggatcagact tctcctcggc gacgacgcac agccgctctt cgagggcgtg cgcccggtcg
     3541 acccgctgaa gttcaaggac ggcaagcgtt accgcgaccg cctgcgggac acggaggcaa
     3601 agacggggcg gccggacgcg atccaggccg cgcagggcga gatcgacgga gtcccggcgg
     3661 tcgtcgccgt cctcgactac gcgttcatgg gcggctcgat gggttcggtc gtgggcgagg
     3721 tgctcacgcg ctgcgccgag cacgccctca aggaacgaaa ggcctacgtg atcgtgagcg
     3781 cctccggcgg cgcgcgcatg caggagggaa tcctctcgct catgcagatg ggcaaggtct
     3841 ccgcggcgct cgcacgcctc tcggccgcgc ggattcccta cgtctccgtc ctcacggacc
     3901 ccacgacggg cggcgtcacg gcgtcctttg cgatgctcgg cgacgtgaac atggccgaac
     3961 cgaaggcgct catcggcttc gcgggcccgc gcgtcatcga gcagacgatc cgccagacgc
     4021 tgccggaagg cttccagcgg agcgagttcc tgcaggacca cggattcgtc gacctcgtcg
     4081 tcccgcggcc cgagatgcgc gcgacgctcg cgcgcgtgct gcggctgctg cagacggcct
     4141 gatggccgag cccgggggac gaagattcgg attttcttag aaggttagtg gggcgagggg
     4201 cttgagcgcc gtcgtcgctt ctccggggct ggcgtggctc gacgctctcg gcccgcagcg
     4261 catccgtccc ggtctctcgc gaacgctttc tctcttagct tctctaggaa atccccaatc
     4321 ttctttctct tcgatcctca tcggagggac gaacgggaag gggtcgaccg ccgccacgat
     4381 ttccgcgatc ctcacggccg cggacgtgcc gtgcgggctc tacacgtcgc cgcacctcgt
     4441 gtccgtgacc gaacgcgtgc gcctgcacga ccgcgacgtt tcggtcgcgg cgctcgacgc
     4501 cgcgctttct ctcgtcgcgg cggtctcggc gcccggcgac cgctcgccga cgtacttcga
     4561 ggcgatgacg gtcgcggcgt tcgagctgtt ccgccgcgca cgcgtcgcgg tggcggtcgt
     4621 cgaggtgggg atcggcgggc ggctcgacgc gacgaacgtg ctcgatcccg aggtttccgt
     4681 cgtcacgaac gtcgcggcgg atcatctcga cgtcctcgga cccgcgctcg aggacgtggc
     4741 acgcgagaag gccggcatct tccgcagggg ccagcccgcg ctcgtcgggg cggagggcac
     4801 cgaggagcgg ccgcgcgccg tgttgcacgc cgaggcccgc agggtcggcg cgcgcctcgt
     4861 cgagattccg cacgcgccga actgggaggg cctcacga
//
LOCUS       DAOMFY010000292         4886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6152_length_4886_cov_6.628648, whole genome shotgun sequence.
ACCESSION   DAOMFY010000292 DAOMFY010000000
VERSION     DAOMFY010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6152_length_4886_cov_6.628648"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLB01_17045"
     CDS             <1..855
                     /locus_tag="PLB01_17045"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQQ79063.1"
                     /translation="LDRAGVARVPGARRAWAFLHGLVFRSGERRIVVNGMPMWVDTRD
                     RVIATRLVGEGVWEPAETAAFRARAREGMCVFDVGANIGYYTLLAARAVGPSGAVYAF
                     EPEPRNFELLARNVAENGFTNVRLFNAAVSSRPGSARLHLDDANFGAHAFEAASVRTS
                     SGRSIDVAAVTLDGFAGDARGFPAGVLVKVDVQGAEALVVAGGRGLLALPGVSLFLEL
                     WPVALERAGADPARLLADLEGLGFSFEDVGVPEKDRRPLGPAEILEACRTRTQAWMNL
                     LLTKRVAR"
     gene            860..2002
                     /locus_tag="PLB01_17050"
     CDS             860..2002
                     /locus_tag="PLB01_17050"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQQ79064.1"
                     /translation="MKKVVVLGAGLVGGFVARTLAEDGFDVTALDFSEAALGALAGVP
                     RLSTKRADLSSPSVLAEAVAGADVVVGAVPGFLGHAMLKTVLDCGKPVSDISFAPEDP
                     LVLDALAKAKGAAAIVDCGVSPGLSNLACGRAAARFDAVDSIVISVGGLPVKRVPPWE
                     YCAVFSPTDVLEEYTRPARLVVDGRVVTKPALSDVQPFEAPGMGTLEGFLSDGLRTVL
                     TTVKAKNLCERTLRWPGHAEKARFLREAGFFSDELVDVAGARVAPRAVAEALIFPQWK
                     RKPGEEEFTVLRVESHGSRGGASFRLVHGLFDRTDPKTGATSMARTTGYPCVIGAHLL
                     AGGEFRAPGVHPPENLGRDAKLWDAFVSGLAARGIAFTEEEAAWPR"
     gene            2011..3936
                     /locus_tag="PLB01_17055"
     CDS             2011..3936
                     /locus_tag="PLB01_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ79065.1"
                     /translation="MGRLPNPERAGGKLRNEGPKPPKVERLRALVPDIKALVAPRKGI
                     LALGMVLMAVNRVSGLVLPASTKFLIDDVIGKRHTEMLLPLLAAVVTATLIQGVTSFS
                     LTQLLSKAAQRLIAELRRKVQAHVGRLPVAYFDANKTGALVSRIMSDVEGVRNLIGTG
                     LVEFAGGLLTAAIALVVLFKISATMTLMAFAFLVGFGVVLNKAFGTIRPIFRERAKLH
                     AEVAGRLSESLGGVRVVKGYHAEEREEKTFAAGVQRLLDNVLKTLTSTSVMSLSSTVL
                     LGIVGAVVMFVGARQILAGTLTLGGFFTYTVFLGFLVAPIFQIVAIGTQLTEALAGLE
                     RTREILAEKPEDADPRRTTSLAAIAGDLVFEDVRFAYDEGKEVLHGVSFRAAPGTVTA
                     LVGPSGSGKSTIIGLVAAFHGPTGGRVLVDGVDLSTVRLDSYRTALGVVLQETFLFDG
                     TIRENVAFSRPDAPEEQILEACRIARVDEFAEGFEKKYDTIVGERGVKLSGGQRQRVS
                     IARAILADPRILILDEATSSLDSESEALIQQGLSYLMKGRTTFVIAHRLSTIRRADQI
                     LVVENGLVVEHGTHETLFAARGRYWDMYTKQHGVESNLFLAPGEGDPKPRETEEDAPA
                     VPPALAEAVDTLRRPNG"
     gene            3929..>4886
                     /locus_tag="PLB01_17060"
     CDS             3929..>4886
                     /locus_tag="PLB01_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79066.1"
                     /translation="MAEGPASRPRHLSLLALVVVVALPYIGRAVATADGAEVVSESLG
                     LFVQGGFGFGRMPPADATTPEVAPPHHSKYGLFPSLLPLPGLGAAWPIRRTIGAAGVD
                     ALAALTWSAGVLLAGLAFGQLVRALKPGATGPWEAAFVAGTFLWPYAADSFVEPFAAA
                     GLAWGAALLLSDRSPSLAAVPWAGACLLKPILWATTPVLILVVVLDARALRSGGRALR
                     AMAILAAALCLHAAANRLLYGSFVEAGYGEEMLRFTTPIGTGLFGLLLSPGRGLLLFA
                     PVVVAGVVGLRLAPRAAVWLCAGAPLVHLLVVARWWSWEGGTA"
BASE COUNT          598 a         1781 c         1734 g          773 t
ORIGIN      
        1 ctcgaccgcg ccggcgtcgc gcgcgtgccc ggcgcgcggc gcgcgtgggc gttcctgcac
       61 ggcctcgtgt tccggagcgg cgagcgtcgg atcgtcgtga acggcatgcc catgtgggtg
      121 gacacccgcg accgcgtgat cgcgacgcgc ctcgtcggcg agggggtctg ggagccggcc
      181 gagaccgccg cgttccgggc ccgcgcccgg gaggggatgt gcgtcttcga cgtcggcgcg
      241 aacatcggct actacacgct gctggcggcg cgcgccgtgg ggccgtcggg cgccgtgtac
      301 gcgttcgagc ccgagccccg gaacttcgag ctgctcgcac ggaacgtcgc cgaaaacggg
      361 ttcacgaacg tgcggctgtt caacgccgcc gtctcgagcc gtcccggctc cgcgcggctc
      421 cacctggacg acgcgaactt cggagcgcac gcgttcgagg ccgcctccgt tcgcacgtcg
      481 tcaggccgct cgatcgacgt cgcagccgtg accctcgacg gcttcgccgg cgacgcccgc
      541 ggcttcccgg cgggagttct cgtgaaggtc gacgtccagg gcgccgaggc tctcgtcgtc
      601 gcgggagggc gcgggctcct cgcgcttccg ggcgtctcgt tgttcctgga gctctggccc
      661 gtggcgctcg aacgggcggg ggccgacccg gcgcgtctcc tcgccgacct ggaaggcctc
      721 ggcttctcgt tcgaagacgt cggcgtgccc gaaaaggacc gtcgcccgct cgggccggcc
      781 gagattctcg aggcctgccg gacgcgcacc caggcgtgga tgaacctgct cctcacgaag
      841 cgcgtggcaa gatgacgcca tgaagaaggt cgtcgtcctc ggcgccgggc tcgtcggtgg
      901 tttcgtggcg cggacgctcg cggaggacgg gttcgacgtg accgccctcg acttcagcga
      961 ggccgcgctc ggcgcgctcg cgggagtccc gcgcctctcg acgaagcgtg cggacctttc
     1021 cagcccgtcc gtcctcgcgg aggcggtcgc gggcgcggac gtcgtcgtgg gcgcggtgcc
     1081 gggcttcctc ggccacgcga tgctcaagac ggtcctcgac tgcggcaagc cggtctcgga
     1141 catctccttc gccccggagg atcccctcgt gctcgacgcg ctcgcgaagg cgaagggcgc
     1201 cgccgcgatc gtggactgcg gcgtgtcgcc gggcctctcc aacctcgcct gcggccgggc
     1261 cgcggcgcgt ttcgacgcgg tcgactcgat cgtgatctcg gtgggcggcc tccccgtgaa
     1321 gcgggtgccg ccgtgggagt actgcgccgt cttctcgccc acggacgtcc tcgaggagta
     1381 cacgcgcccg gcccgcctcg tcgtggacgg gcgcgtcgtc acgaagccgg cgctgtccga
     1441 cgtccagccg ttcgaggcgc cgggtatggg cacgctcgag ggcttcctga gcgacggcct
     1501 gcgcacggtc ctgacgacgg tgaaggcgaa gaacctctgc gagcggacgc tgcgctggcc
     1561 gggccacgcg gagaaggcgc gcttcctgag ggaggccgga ttcttctccg acgagctcgt
     1621 cgacgtcgcg ggagcccggg tggccccgcg cgccgtcgcg gaggcgctca tcttcccgca
     1681 gtggaagagg aagcccggcg aggaggagtt cacggtgctg cgggtcgagt cgcacgggtc
     1741 gcgcggcggc gcgagcttcc gtctcgtcca cggcctcttc gaccgcaccg acccgaagac
     1801 gggggcgacg tcgatggcgc gcacgacggg ctatccgtgc gtgatcgggg cgcaccttct
     1861 cgccgggggc gagttccgcg cgcccggagt ccatcccccc gagaacctcg gccgtgacgc
     1921 gaagctgtgg gacgcgttcg tctcgggcct cgcggcgcgc ggcatcgcgt tcacggaaga
     1981 agaggccgcg tggccgcgat aaagagcccg atgggccgcc tcccgaaccc cgagcgggcg
     2041 ggaggcaagc tccgcaacga ggggccgaag ccgccgaagg tcgagcgcct ccgggccctg
     2101 gtgcccgaca tcaaggcgct cgtcgcgccg cgaaagggga tcctggcgct cgggatggtc
     2161 ctcatggcgg tcaaccgcgt ctcgggtctc gtcctccccg cctcgacgaa gttcctgatc
     2221 gacgacgtga tcggcaagcg ccacacggag atgctgctgc ctctcctcgc cgcggtcgtc
     2281 acggcgacgc tgatccaggg cgtcacgtcg ttctcgctga cccagctcct gtccaaggcc
     2341 gcgcagcggc tcatcgcgga gctgcggcgc aaggtgcagg cgcacgtcgg gcggctcccc
     2401 gtcgcgtatt tcgacgcgaa caagaccggc gcgctcgtct cgcggatcat gagcgacgtc
     2461 gaaggcgtcc ggaacctcat cgggacgggc ctcgtcgagt tcgcgggcgg cctgctcacg
     2521 gccgcgatcg cgctcgtcgt cctcttcaag atcagcgcga cgatgacgct catggccttc
     2581 gcgttcctcg tggggttcgg cgtcgtcctg aacaaggcgt tcgggaccat ccggccgatc
     2641 ttccgcgagc gcgccaagct ccacgcggag gtcgcagggc gcctctccga gtcgctcggc
     2701 ggcgtaaggg tcgtcaaggg ctatcacgcc gaggagcgcg aggagaagac gttcgcggcc
     2761 ggcgtgcagc gcctcctcga caacgtcctg aagacgctga cctcgacgag cgtcatgagc
     2821 ctctcgtcga ccgtgctcct cggcatcgtg ggcgccgtcg tgatgttcgt cggcgcgcgc
     2881 cagatcctcg ccggaacgct cacgctcggc ggcttcttca cgtacacggt cttcctcggg
     2941 ttcctcgtgg ccccgatctt ccagatcgtc gcgatcggga cgcagctcac cgaggccctc
     3001 gccggcctcg agcgcacgcg cgagatcctc gccgagaagc ccgaggacgc cgatccccgg
     3061 cgcacgacgt ccctcgccgc gatcgcgggc gacctcgtgt tcgaggacgt ccggttcgcc
     3121 tacgacgagg gcaaggaagt cctccacggc gtctcgttcc gcgccgcgcc cggcacggtc
     3181 accgccctcg tcggcccgtc cggatcgggc aagtccacga tcatcggcct cgtcgccgcc
     3241 ttccacggcc ccacgggcgg ccgcgtcctc gtggacggcg tcgacctctc gaccgtgcgg
     3301 ctggactcgt accgcacggc tctgggcgtc gtcctgcagg agacgttcct cttcgacggg
     3361 acgatccggg aaaacgtcgc gttctctcgt cccgacgccc cggaagagca gatcctcgag
     3421 gcctgccgga tcgcgcgcgt ggacgagttc gccgagggct tcgagaagaa atacgacacg
     3481 atcgtcggcg agcgcggcgt caagctgtcc ggcgggcagc gccagcgcgt ctcgatcgcg
     3541 cgcgcgatcc tcgcggaccc gcgcatcctg atcctcgacg aggcgacgtc cagcctcgac
     3601 tccgagtccg aggccctcat ccagcagggg ctgtcttacc tcatgaaggg ccggacgacg
     3661 ttcgtgatcg cccaccgcct gtcgacgatc cgccgcgcgg accagatcct cgtcgtcgag
     3721 aacggcctcg tcgtcgagca cggcacgcac gagacgctct tcgccgcccg cgggcggtac
     3781 tgggacatgt acacgaagca gcacggtgtc gagagcaatc tcttcctcgc tccgggcgaa
     3841 ggcgatccga agccgcgcga gacggaggag gacgctccgg cggtcccgcc ggcgctcgcg
     3901 gaggccgtcg acacgctgcg ccgccccaat ggctgaaggc ccggcctcgc gaccgaggca
     3961 cctttctctc ctcgcgctcg tcgtcgtcgt cgccctgccg tacatcggga gagcggtggc
     4021 gacggccgac ggggcggaag tcgtttcgga atcgctgggg ctcttcgtgc agggcgggtt
     4081 cggcttcggc cggatgcctc cggccgacgc gacgactccc gaggtcgcgc cgccgcacca
     4141 ctcgaaatac ggcctgttcc cgtccctcct gccgctgccc ggcctgggcg ccgcgtggcc
     4201 gatccgccgg acgatcggcg ccgcgggcgt cgatgcgctt gcggcgctca cgtggtcggc
     4261 cggggtcctg ctcgccggtc tcgcgttcgg gcaactcgtg cgcgccctca agcccggcgc
     4321 gacggggccc tgggaggcgg cgttcgtcgc cgggacgttt ctctggccgt acgcggcgga
     4381 ttccttcgtc gagccgttcg cggccgcggg tctcgcctgg ggagccgcgc tccttctctc
     4441 ggaccgctcg ccctccctcg ccgccgttcc gtgggcgggc gcgtgtcttc tcaaaccgat
     4501 cctgtgggcg acgacgccgg tcctgatcct cgtcgtcgtc ctcgacgccc gggcccttcg
     4561 ctcaggcggg cgggccctcc gcgcgatggc gatcctcgcg gccgccctgt gcctccacgc
     4621 cgcggccaac cggcttctct acggatcgtt cgtcgaggcc ggttacggcg aggagatgct
     4681 ccgcttcacg acgccgatcg ggaccggcct cttcggtctc ctcctctcgc cgggccgtgg
     4741 ccttctcctc ttcgctccgg tcgtcgtggc gggcgtcgtg ggcctcaggc tcgcgccgcg
     4801 cgcggccgtg tggctctgcg cgggggcgcc gctcgtgcac cttctcgtcg tcgcgcgctg
     4861 gtggagctgg gagggcggca ccgcgt
//
LOCUS       DAOMFY010000293         4855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6209_length_4855_cov_6.218333, whole genome shotgun sequence.
ACCESSION   DAOMFY010000293 DAOMFY010000000
VERSION     DAOMFY010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6209_length_4855_cov_6.218333"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLB01_17065"
     CDS             complement(<1..408)
                     /locus_tag="PLB01_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79067.1"
                     /translation="MKSTRFVMFVVLAVALCVAGAAVAQTVVKSEVKSGLVIGKTDHS
                     VIIKGDDGVVKEYQVPAGRTAMVDGKEVGLADLKIGTTLSATFNTVEKPVEVKTTTIK
                     NGEVVRVQGANLVTKEADGFHTYVVPKGFKFNVD"
     gene            566..2368
                     /gene="uvrC"
                     /locus_tag="PLB01_17070"
     CDS             566..2368
                     /gene="uvrC"
                     /locus_tag="PLB01_17070"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQQ79068.1"
                     /translation="MGAPPDLSLYPDQPGIYLYKDAAGKILYVGKARSIRKRLASYFH
                     AGPKHPRTEALLAEFATIDTVVSKTESEALALENAFIKKNRPRYNIVLRDDKTYPYIK
                     VTTGEAWPRAFVTRKVLKDGHSYFGPFLPARTCRGAMKMVQRMFQIRTCRIEIDGKLP
                     RPCLYYDMHACLGPCVSGLTTAEAYADAVQDVLLFLSGRNDELRPRLEEKMRAAADAE
                     RFEMAAAYRDCLRTVAELAEKQRVQSVSGEDVDVFGDFFDGGNLAVAVLLVRGGVILD
                     SREYFWERIGDVPPEEFWVSLLSQYYDSVASSFLPREVHVPVDVTDEALEPLEAWLSE
                     RRGSRVTIRTPRRGAAFDRVRIARDNARQNHVRRFRRVRERGEKATLALARALELPAR
                     PARIECFDISHFQGAETYASCVVFVDGEPKRDEYRLFRIEREGVDDFASISEAVTRRY
                     APAVEERRPLPDLLLIDGGKGQLSAALAALDRLGLEMPAAGLAKREEEVFVPGRSAPI
                     VLARSNAGLKLLQRARDEAHRFGLKSHRRARGKRALASPLLDVPGLGPARVKTLLAAF
                     GGAEAVLAASEDALAVVSGKSVAARIARWRSATR"
     gene            2448..3917
                     /locus_tag="PLB01_17075"
     CDS             2448..3917
                     /locus_tag="PLB01_17075"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HQQ79069.1"
                     /translation="MTGGSLERTPFAEVIRMLYRERATAKLLVSYRGEERTFWFDRGQ
                     ILSASSNREAQLVGDLLRTFGLADESLLMSAFEKALAEPGRGLARALTESGAVADYVA
                     DAAVRALSERLFFETFRWTNGVFTVSPLESAIDVPVRADRPTGGLLLEALRRLPAPAA
                     QLNTPLSPAARPVFAPDLLMRYQAFTVSAVEAEVLGRIDGSMTVEQIHPDQKVVARLA
                     ATGLVHLVAPGRTVEKSGVPEGLLLLNVDVAGAAPAARLAEQSDIQRDLVRRTYRRLD
                     WASHYDVLGVERDVAPEALARAIHERARLFHPDHHLKPHLADDRDALEALFNRVREAE
                     RTFRSPESRAQYDRTQEIHGDTVALPGTEPTVEVQKDIARKNYVRAKALYEEEDYFPA
                     YEMIRQAVEFDPEQKEYWVLLSRIQRKNPKWIRQATETLRRAVKKMPDSVDLWWELAE
                     ACAHERNEPDRVKALKEVMRLDPVNRRAQQALAEINAKS"
     gene            3919..4530
                     /locus_tag="PLB01_17080"
     CDS             3919..4530
                     /locus_tag="PLB01_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HQQ79070.1"
                     /translation="MNGLPQLFGTGLGLSAAAGLNSYAVLLVYGAMARFFPEDYTGSI
                     ARLLASTPALVVAAVLFLLEFFADKIPGLDHFWHLLHSIVRPLVGALVAIAAVQPEGS
                     AALDVVAGGAGGGVALASHLVKSATRLTSTAVTFGTANAGLSLAEDVVAFLQSLVSIF
                     LPLVALVVVFAIGVLFVLTVPRMARFLDLFGRRRTRREPSPPA"
     gene            4664..>4855
                     /locus_tag="PLB01_17085"
     CDS             4664..>4855
                     /locus_tag="PLB01_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79071.1"
                     /translation="MDDLRDVAAVPVEVSRSVDVGRLDGDVRFTRAILVLPARDAAGL
                     ERLLASQQDPSSPEFRRWLA"
BASE COUNT          687 a         1727 c         1631 g          810 t
ORIGIN      
        1 gtccacgttg aacttgaagc ccttcggaac gacgtacgtg tggaagccgt cggcctcctt
       61 cgtgacgagg ttggcgccct ggacgcgcac gacttcgccg ttcttgatcg tggtcgtctt
      121 gacctcgacg ggcttctcga ccgtgttgaa cgtggcggag agcgtcgtgc cgatcttgag
      181 gtcggcgagg ccgacttcct tgccgtcgac catggcggtg cggccggccg ggacctgata
      241 ctccttcacg acgccgtcgt cccccttgat gatgacggag tgatcggtct tgccgatcac
      301 gaggccggac ttgacctccg acttgacgac ggtctgcgcg accgcggcgc ccgcgacgca
      361 cagggcgacc gcgaggacga cgaacatgac gaaccgggtg gatttcattc aattcctcct
      421 gctgaattgg ttcgctcggg gcgaacgaca gtggatagac gttgacccat tctgccctat
      481 gtgagtaccc aggaggggtt ctgtcaatag ttcaagcgct tcggtgaatt cagcgccggc
      541 cgcccgcctc cttagaatcg gtccggtggg cgcccctccg gacctctcgc tctatcccga
      601 ccagccgggc atctacctgt acaaggacgc cgcggggaag atcctctacg tcggcaaggc
      661 gcgctcgatc cgtaagcgcc tcgcgtccta tttccatgcg ggaccgaagc acccgaggac
      721 cgaggcgctc ctcgccgagt tcgccaccat cgacacggtc gtctcgaaga ccgagagcga
      781 ggcgctcgcc ctcgagaacg cgttcatcaa gaagaaccgg ccgcgctaca acatcgtcct
      841 ccgcgacgac aagacctacc cctacatcaa ggtgacgacg ggggaggcct ggccgcgggc
      901 tttcgtgacg cgaaaggtcc tcaaggacgg ccacagctac ttcggcccct tccttccggc
      961 ccggacgtgc cgcggcgcga tgaagatggt gcagcggatg ttccagatcc gcacctgccg
     1021 catcgagatc gacggcaagc tcccgcgccc gtgcctctac tacgacatgc atgcctgcct
     1081 cgggccgtgc gtttcaggcc tgaccacggc cgaggcctac gcggacgcgg tccaggacgt
     1141 cctgctcttc ctgtcggggc ggaacgacga gctgcgcccg cggctcgagg agaagatgcg
     1201 cgccgctgcc gacgccgaga ggttcgagat ggcggccgcc taccgcgact gcctccgcac
     1261 ggtcgcggaa ctcgccgaga agcagcgggt ccagtccgtg tcgggcgagg acgtcgacgt
     1321 cttcggagat ttcttcgacg gaggcaatct ggcggtcgcg gttctcctcg tgcgcggagg
     1381 cgtgattctc gattcgcgcg agtatttctg ggagcggatc ggcgacgttc ctcccgagga
     1441 attctgggtt tctctccttt cgcagtatta cgactccgtg gcttcgagct ttctgccccg
     1501 ggaggtccat gttcccgtgg acgtcacgga cgaggccctc gagccgctcg aggcgtggct
     1561 gtcggagcgc cggggctccc gggtgacgat ccgcacgccg cgccggggcg ccgcgttcga
     1621 ccgcgtccgg atcgcgcgcg acaacgcgcg acagaaccac gtgcgccgct tccggcgggt
     1681 ccgggagcgg ggagagaagg cgacgctcgc gctcgcccgg gccctcgagc tgcccgcgag
     1741 gcccgcgcgg atcgagtgct tcgacatctc ccattttcag ggcgccgaaa cctacgcgtc
     1801 gtgcgtcgtg ttcgtggacg gcgagccgaa gagggacgag taccgcctct tccggatcga
     1861 acgggagggc gtggacgact ttgcgtcgat ctcggaggcc gtcacgcggc gctacgcgcc
     1921 cgcggtggag gagcggcgtc ctctgccgga cctccttctg atcgacggcg gcaagggcca
     1981 gctctcggcc gcgctcgcgg cgctcgaccg cctcgggctc gagatgccgg ccgcgggcct
     2041 cgcgaagcgc gaagaggagg tgttcgtgcc ggggcgctcg gcgccgatcg tgctcgcccg
     2101 gtcgaacgcg ggcctcaagc tgctccagcg cgcgcgggac gaggcgcatc gcttcggcct
     2161 gaagtcccac cggcgagccc gcggcaagcg cgcactcgcg agcccgctgc tcgacgtgcc
     2221 cggcctcggc cccgcgcgcg tgaagacgct tctcgcggct ttcggcggcg cagaagcggt
     2281 cctcgccgcc agcgaggacg ccctcgcggt cgtgtcgggc aagagcgtcg ccgcgcgcat
     2341 cgcgcgctgg cgctcggcga cccgctgatt ctcgcgggcc ggaagccttc gcgctgctac
     2401 actttgcgcc gatggtcgcg ccgcgttcgg actcgcgtaa tcgcatgatg acgggaggct
     2461 ccctcgagcg cacgccgttc gccgaggtca tccgcatgct ctatcgcgag cgcgcgacgg
     2521 cgaagctcct cgtttcgtac cgcggcgagg agcggacgtt ctggttcgac cgggggcaga
     2581 tcctctcggc gagctcgaac cgggaggcgc agctcgtcgg cgacctcctc cgcaccttcg
     2641 ggctcgccga cgagagcctg ttgatgtccg cgttcgagaa ggcgctcgcc gagccgggac
     2701 ggggcctcgc gcgcgccctc acggaatccg gcgcggtcgc ggactacgtc gcggacgccg
     2761 ccgtccgggc tctttcggag cggctcttct tcgagacgtt ccgctggacg aacggcgtct
     2821 tcacggtgtc cccgctcgag agcgcgatcg acgtgccggt tcgcgcggat cggcccaccg
     2881 gcgggctgct cctcgaagcg ctgcgtcggc tccccgctcc ggcggcccag ctcaacacgc
     2941 cgctctcgcc cgcggcgcgg cccgtcttcg ctccggacct tctcatgcgc tatcaggcat
     3001 tcacggtgtc cgccgtcgaa gccgaggtgc tcgggcggat cgacggctcc atgaccgtgg
     3061 agcagatcca cccggaccag aaggtcgtcg cgcgccttgc ggcgacgggc ctcgtccacc
     3121 tcgtggcgcc gggacgtacg gtggagaaat cgggcgtccc ggaggggctg cttctcctga
     3181 acgtcgacgt ggccggcgcc gcgccggcgg cgcgcctcgc cgaacagtcc gacatccagc
     3241 gcgacctcgt gcgccgcacg taccgccgcc tcgactgggc gagccattac gacgtgctcg
     3301 gcgtcgagcg ggacgtcgct cccgaggcgc tcgcgagggc gatccacgag cgggcgcgcc
     3361 tgttccaccc cgaccaccac ctcaagccgc atctcgcgga tgaccgggac gcgctcgagg
     3421 ccctgttcaa ccgcgtccgg gaggccgaac gcaccttccg ctcgcctgag agccgggcgc
     3481 agtacgaccg cacccaggag atccacggcg acacggtggc ccttcccggc accgagccca
     3541 cggtcgaggt ccagaaggac atcgcgcgca agaactacgt gcgcgccaag gccctctacg
     3601 aggaggaaga ctacttcccg gcctacgaga tgatccgtca ggccgtcgag ttcgacccgg
     3661 agcagaagga atactgggtg ctcctctcgc gcatccagcg caagaacccc aaatggatca
     3721 ggcaggcgac cgagaccttg cgccgcgccg tcaagaagat gcccgacagc gtcgacctct
     3781 ggtgggagct cgccgaagcc tgtgcgcacg agcgcaacga gcccgatcgc gtgaaggcgc
     3841 tcaaggaggt catgcgtctg gatcccgtga accggcgcgc ccagcaggcg ctcgccgaga
     3901 tcaacgcgaa gtcctgagat gaacggcctc ccgcagctct tcggcaccgg actcggcctt
     3961 tcggccgcgg ccggcctcaa ttcctacgcg gttctcctcg tgtacggcgc gatggcgcgc
     4021 ttcttcccgg aggactacac cggttcgatc gcgcgcctcc tcgcgtcgac ccccgcgctc
     4081 gtcgttgcgg cggtcttgtt cctgctcgag tttttcgcgg acaagattcc ggggctcgac
     4141 cacttctggc atctcctcca ctcgatcgtg cggccgctcg tcggcgcgct cgtcgcgatc
     4201 gctgcggtgc agcccgaggg atcggccgcg ctggacgtcg tggcgggagg agcgggcgga
     4261 ggagtcgcgc tcgcgtcgca cctcgtcaag agcgcgacgc gtctcacgtc gacggccgtc
     4321 acgttcggca ccgcgaacgc ggggctgtcc ctcgcggagg acgtcgtcgc gttccttcag
     4381 agcctcgtct cgatcttcct gcctctcgtc gcgctcgtcg tcgtgttcgc gatcggcgtg
     4441 ctcttcgtcc tcacggtccc ccggatggcg cggtttctcg acctgttcgg acggcgccgc
     4501 acccggcgag aaccgtcgcc gccggcctga ccctgaggaa ccggcacgtc tcctgcatcg
     4561 tctgtcggag gagttttcga tgaagtcccg cctcccgcgc ctggagaccg cagctgccgc
     4621 cgcctttctc ttcggcgcta cctctgcctt cgcgactcgt cccgtggacg acctccgcga
     4681 cgtcgcggcg gtgcccgtgg aggtctcgcg gtccgtggac gtggggcgcc tcgacgggga
     4741 cgtccgcttc acgcgcgcga tcctcgtcct gcccgcgcgc gacgccgccg gcctcgagcg
     4801 gctcctcgcg tcgcagcagg atccgtcctc gccggagttc cggcgctggc tcgcg
//
LOCUS       DAOMFY010000294         4843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6234_length_4843_cov_7.207811, whole genome shotgun sequence.
ACCESSION   DAOMFY010000294 DAOMFY010000000
VERSION     DAOMFY010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6234_length_4843_cov_7.207811"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..489
                     /locus_tag="PLB01_17090"
     CDS             88..489
                     /locus_tag="PLB01_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79072.1"
                     /translation="MTFQPLPHVFPFRFADAVVASAGRAEGRVRATVTAGAQASRGGP
                     LAATLYGELIAQSALLLEGGDADLGRRGFLAGISDLTIVREAAPGDVLDVDLTIAGRF
                     GDSVKFDGRVSDAAGAEVARGSIVVRHGGAA"
     gene            486..1217
                     /locus_tag="PLB01_17095"
     CDS             486..1217
                     /locus_tag="PLB01_17095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQQ79073.1"
                     /translation="MTPDSGTFPRDSGLFAKKKVLLAGPPAGLLRGGASVLSLAGYVV
                     RVAESVEEIEALAPEKIPDLVILDASFPPDGGVAACRRLREKPHWRAVSMMLVVPAGL
                     PHLEESLVPGINDFMLAPFPADELLDKASRLTLIPARREVNTIARVTSEGALKLGKML
                     NVSSNGVLVEIETPIPIGKAVAIEFFLPEDDKPLHASGKVVRRTNDLDHFHEAFGIRF
                     TEISEVDQVRIDSYVAERERAGFRR"
     gene            1270..1358
                     /locus_tag="PLB01_17100"
     tRNA            1270..1358
                     /locus_tag="PLB01_17100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1304..1306,aa:Leu,seq:cag)
     gene            complement(1453..2382)
                     /locus_tag="PLB01_17105"
     CDS             complement(1453..2382)
                     /locus_tag="PLB01_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79074.1"
                     /translation="MRDPHDISSPIRMLGAATIAVTAAAALVAGGCGSRTEHAQLVSE
                     KKYLATENRRVSDALEAKNVQTRELEVALLEVQNGLAEIRGQELAAVRTSIRVAKEGG
                     ASATLREELRTEVRTIRDAVHENLAKLARLEAQSAASGLKTATLERQTGELRRSLEEK
                     DALVKELDARVRDLSLAVKTQAASLKEKDAALREGEARLARKTKEANTAWVAVASKRT
                     LAQKGVVARAGLGGAWTQTGRFDPDVFREVDVTRDLFVEIPAPAKSIRIVTAQPKESY
                     RIVDADAKTSKLEVADAEAFWRGERYLVVMLPD"
     gene            2573..4837
                     /gene="rnr"
                     /locus_tag="PLB01_17110"
     CDS             2573..4837
                     /gene="rnr"
                     /locus_tag="PLB01_17110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQQ79075.1"
                     /translation="MTPNQLRDAALAALRPRGQRVLPFNDLARKVFGLAPSAADEDAL
                     RDAVADLERRGEIVRVRGEKLSLIEFTDFAAGTLAVRGEGRAFLLSGVPGVPDTPIVG
                     LGSALDGDFVLVRVEAARTAPKPKSGRAPRFDPRPSGTVVKVLVRRRETVVGKIVRGP
                     EGTFVVPFDRKIDARLAVPDGKDLNAPDGMFVEARITAYPDDKRLALAEVTETIGFEG
                     DAGVDVEVVARKWGIPRKYPETVIAEAEASVREVGEDERRQRTDFTGRTIVTIDGETA
                     RDFDDAIEAEQLPNGGFRIGIHIADVSHYVPVGSALDAEAYERGTSVYFPDRAIAMLP
                     ERLSNDLCSLRPDEERRTVSAVLTLDRHGETVKSEFYRSLIKSRARLTYTAVGDFLEG
                     KEGSWFPDGVAPMLKVAQKAAHALQSRRVRRGSLDFDLPDSDVILGEEGEAVAILKAV
                     RNEAHRLVEEFMLAANEAVARHLVFVPTPALYRVHDRPDETRLAALRVVLGPLGYDLP
                     EDEEEVTPATFQAILDQAEGKPEERFVDDLVLRSQKKALYSEDCRGHYALAATYYAHF
                     TSPIRRYPDLLVHRALVEWIATRHAPRPEEAEGRTRWFHEASLHCSSRERRAESAERE
                     AVAWKKFVFLSKRVGDEFWAYVSAVVGFGLFVTLEDVYVDGLIPISSLQDDFYRFEDV
                     EHRLVGASTGRVYRLGDRLRVRLVKADSEKRGLDFVPVGSSAGPVSKTRVEALHRQQR
                     KPAARRGPAPRRRR"
BASE COUNT          667 a         1739 c         1678 g          759 t
ORIGIN      
        1 cgggatcaag gtgcaggacg aagaagtcgg ggtgaaggcg ttcgcctcgg tgaacgccct
       61 ctgcgacttc atccgcgcga aggccgcatg acgttccagc cgctcccgca cgtctttccg
      121 ttccgcttcg ccgacgcggt cgtcgcgtcc gccggccggg cggagggccg cgtgcgggcg
      181 acggtgacgg cgggggcgca ggcgtcgcgg ggcgggccgc tcgcggcgac gctctacggc
      241 gagctcatcg cgcagtccgc gctcctcctc gagggcgggg acgcggacct cggccgccgc
      301 gggttcctcg ccggtatctc cgacctcacg atcgtccgcg aggcggcccc gggcgacgtc
      361 ctcgacgtgg acctgacgat cgccggccga ttcggcgatt cggtgaagtt cgacggcagg
      421 gtttccgacg ccgccggcgc cgaggtggcg cgcgggtcga tcgtggtgcg gcacgggggc
      481 gcggcgtgac cccggactcc gggactttcc cgcgagactc cgggctcttc gccaagaaga
      541 aggtcctgct tgcgggtcct ccggcgggcc tcctccgggg cggcgcgtcg gtcctctctc
      601 tcgcgggcta cgtcgtacgc gtcgccgaga gcgtcgagga gatcgaggcg ctcgcgcccg
      661 agaagatccc cgatctcgtc atcctcgacg cctcgtttcc gccggacggg ggcgtcgcgg
      721 cctgccgtag gctgcgcgag aagccgcatt ggcgagccgt ttcgatgatg ctcgtcgtcc
      781 cggccggcct cccgcacctc gaggagtcgc tcgtcccggg catcaacgac ttcatgctcg
      841 cgccgtttcc ggccgacgag ctcctcgaca aggcgagccg tctcacgctg atccccgcgc
      901 ggcgcgaggt caacacgatc gctcgcgtca cgagcgaggg ggcgctgaag ctcggaaaga
      961 tgttgaacgt ctcctcgaac ggggtgctcg tcgaaatcga gacgccgatc ccgatcggga
     1021 aggcggtcgc gatcgagttc ttcctgcccg aggacgacaa gcccctccac gcgtcgggga
     1081 aggtcgtgag gcgaacgaac gacctcgacc acttccacga ggcgttcggg atccgcttca
     1141 ccgagatctc agaggtcgac caggtccgga tcgacagcta cgtcgccgag cgcgagcggg
     1201 ccggcttccg gcggtagtgc gcccccaaat ttgacgccgt ctcgcccgcc gcttatcctc
     1261 ctcgcccgcg cgaaagtggc ggaattggta gacgcgcaag gctcaggacc ttgtagccct
     1321 taccggctgt gggggttcga gtcccccctt tcgcaccagc ctgaacgaac ggccccgcct
     1381 caccggcggg gcttttcgtt gggcaacctg cccccgggcg cccggggcag gggacgggca
     1441 ggtccgccgg actcagtcgg ggagcatgac gacgaggtaa cgctcgcctc tccagaacgc
     1501 ctcggcgtcg gcgacctcga gcttcgacgt cttcgcgtcc gcatcgacga tccggtagct
     1561 ctccttcggc tgcgccgtca cgatgcggat gctcttcgcg ggcgccggga tctcgacgaa
     1621 gaggtcccgc gtcacgtcca cctcgcggaa gacgtccggg tcgaatcggc ccgtctgcgt
     1681 ccacgccccg ccgaggcccg cgcgtgcgac gacgcccttc tgcgcgagcg tgcgcttcga
     1741 agcgaccgcg acccaggccg tgttcgcctc cttcgtcttg cgggcgagcc gcgcctcgcc
     1801 ttcgcgcagg gcggcgtcct tctctttcag gctcgccgcc tgcgtcttca cggcgaggga
     1861 gaggtcgcgg acgcgcgcgt cgagctcctt cacgagggcg tccttctcct cgagagaccg
     1921 gcgcagctct cccgtctgcc tctcgagagt tgcggtcttg aggccgctcg cggcgctctg
     1981 cgcctccagg cgggcgagct tcgcaaggtt ctcgtggacc gcatcccgga tcgtccggac
     2041 ctcggtccgc agctcctcgc gcagggttgc cgaggccccg ccctccttcg cgacgcggat
     2101 cgacgtgcgg accgcggcga gctcctggcc gcggatctcg gcgaggccgt tctgaacctc
     2161 gaggagcgct acttcgagct cgcgcgtctg gacgttcttc gcctcgaggg cgtcggagac
     2221 gcggcggttt tccgtcgcca ggtatttttt ctccgagacg agctgtgcgt gctccgttct
     2281 ggagccgcag ccgcctgcga cgagcgcggc ggccgccgtg acggcgatcg tcgcggcgcc
     2341 caacatcctg atggggctgg agatgtcgtg ggggtctctc atggcgtcct cctcgtgcac
     2401 ccccggaatc gcaaagggtg taccggcctc gaggcgcgcg tgtccgtctc cgtggtagcc
     2461 ttgtcccggt cgagcgcggc atcgggcatc cggcccggaa tcgcccgctc ggccagaaat
     2521 tccctgccca gagtcattta ggctcgagac ggccgggttc gagaccccca gaatgacacc
     2581 caatcagctc cgtgacgcgg cccttgcggc tctcaggccg agaggccagc gcgtccttcc
     2641 gttcaacgac ctcgcccgca aggtcttcgg cctcgcaccc tcggcggcgg acgaggatgc
     2701 gctgcgcgac gccgtcgccg acctcgagcg gcgcggcgag atcgtgcgcg tgcgcggcga
     2761 gaagctctcc ctcatcgagt tcaccgattt cgcggccggc acccttgccg tgcgcggcga
     2821 aggccgggcg ttcctgctct ccggagttcc gggagttccg gacacgccga tcgtcgggct
     2881 cggcagcgcc ctcgacgggg atttcgtcct cgtgcgcgtc gaggcagcca ggaccgcgcc
     2941 gaaaccaaag tccggccgcg caccccgttt cgacccgcgc ccgtccggga ccgtggtcaa
     3001 ggtgctcgtc cgccggcgcg agacggtcgt cggcaagatc gtgcgcggac cggaggggac
     3061 gttcgtcgtc ccgttcgacc gcaagatcga cgcgcgcctc gcggtgccgg acgggaagga
     3121 cctgaacgcg ccggacggga tgttcgtcga ggcccgcatc acggcgtacc cggacgacaa
     3181 gcgcctcgcg ctcgccgagg tgaccgaaac gatcggcttc gagggcgacg cgggcgtgga
     3241 cgtcgaggtc gtcgcccgca agtgggggat cccgcggaag taccccgaga ccgtgatcgc
     3301 ggaggccgag gcctccgtgc gcgaggtggg cgaggacgag cggcgccagc ggacggactt
     3361 cacgggccgc acgatcgtca cgatcgacgg cgagaccgcg cgcgacttcg acgacgcgat
     3421 cgaggccgaa cagctcccga acggcgggtt ccggatcggc atccacatcg cggacgtctc
     3481 gcactacgtc ccggtcggga gcgcgctcga cgccgaggcc tacgagcgcg gcacgtcggt
     3541 ctacttcccg gaccgcgcga tcgccatgct tcccgagcgc ctctcgaacg acctctgctc
     3601 gctgcggccg gacgaggagc gccgcaccgt gtccgccgtc ctcacgctgg acaggcacgg
     3661 cgagacggtc aagagcgagt tctaccgctc gctcatcaag tcacgggcgc gtctcacgta
     3721 caccgcagtc ggggatttcc tggagggtaa agaggggagc tggttcccag acggcgtcgc
     3781 gcccatgttg aaggtcgccc agaaggccgc gcatgcgctg cagtcgcgcc gcgtgcggcg
     3841 cgggtcgctg gacttcgacc tgccggactc ggacgtcatc ctcggcgagg agggcgaggc
     3901 ggtcgcgatc ctgaaggccg tgcgcaacga ggcccaccgc ctcgtcgagg agttcatgct
     3961 cgccgccaac gaggcggtgg cgcggcacct cgtcttcgtc ccgacgcccg cgctctaccg
     4021 cgtccacgac cgccccgacg agacgcgcct cgcggcgctg cgcgtcgtcc tcggtccgct
     4081 cggctacgac ctcccggagg acgaggagga ggtcacgccc gcgacgttcc aggcgatcct
     4141 cgaccaggcg gagggaaagc ccgaggagcg cttcgtggac gacctcgtcc tgcgctcgca
     4201 gaagaaggcc ctctactcgg aggactgccg cggacactac gcgctcgccg ccacgtacta
     4261 cgcgcacttc acgtccccga tccgccggta cccggacctc ctcgtccacc gcgcgctcgt
     4321 ggagtggatc gcgacccgtc acgctccgcg gcccgaggag gccgagggcc gcacgcgctg
     4381 gttccacgag gcctcgctcc actgctcgtc gcgcgagcgg cgcgccgagt ccgccgagcg
     4441 cgaggcggtc gcgtggaaga agttcgtctt cctctccaag cgcgtcggcg acgagttctg
     4501 ggcgtacgtg tcggccgtcg tcggtttcgg cctcttcgtc acgctcgagg acgtctacgt
     4561 cgacggcctc attccgatct cgtccctgca ggacgacttc taccggttcg aggacgtcga
     4621 gcaccgcctc gtcggcgctt cgacgggacg ggtctaccgc ctcggagacc ggctgcgcgt
     4681 caggctcgtg aaagccgatt ccgagaagcg cgggctcgat ttcgtcccgg tgggctcgag
     4741 cgcgggtccc gtcagcaaga cgcgcgtcga agcgctccat cgccagcaac gaaagcccgc
     4801 cgcgcgccgc gggcccgccc cgcgcaggcg gcgctgatgg ccg
//
LOCUS       DAOMFY010000295         4817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6293_length_4817_cov_6.536959, whole genome shotgun sequence.
ACCESSION   DAOMFY010000295 DAOMFY010000000
VERSION     DAOMFY010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_4817_cov_6.536959"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLB01_17115"
     CDS             complement(<1..1166)
                     /locus_tag="PLB01_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQQ79076.1"
                     /translation="MAKVLGIDLGTTNSCVAVVEVGSPQVVPNREGARTTPSIVGFTE
                     DGERLVGQIAKRQAITNPTQTIYAVKRLIGRKWTDAEVKHAREHLSYTLVEATNGDVK
                     IRVRDRDYSPEEIAAFVLREIKEFCEEALGEEIKEAIITVPAYFDDSQRQATRDAGRI
                     AGLEVLRIINEPTAAALAYGLEKNRKQETIAVYDLGGGTFDISILRIDNGMFEVLSTS
                     GDTFLGGEDFDRAIMDWLLAEFLQSSGIDLRQDRLALQRLKEAAEKAKCELSSTLETM
                     VALPFIASDASGPKHINKLLTRDTFEALAAELIARTEGPCKNALEAAGLSPDQVDTVI
                     LVGGQTRTPKVQQMVETIFGKPPNRSINPDEVVAVGAAIQAGVLRGDIKDLVLL"
     gene            complement(1159..1950)
                     /gene="grpE"
                     /locus_tag="PLB01_17120"
     CDS             complement(1159..1950)
                     /gene="grpE"
                     /locus_tag="PLB01_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQQ79077.1"
                     /translation="MTGRKRRDEDDGIIFLETESRDAVAKALEEAERAIEAVEERQRQ
                     KQDESASARVPDPPPERPPAPVPQAQPPDPRLAAAEAELAAAIDRARRAHDELEGTKE
                     RAIRAEEEAGRLREALLRKSADFDNLKRRTEKDKAEYFKFALADAFNDLLGVLDNFER
                     ALAHRGDASSEDFQAGIDMIARQLGESLRRYGLVEVPALGLPFDPNVHEAVVREETAD
                     TAPGTVLEVFQKGYMLNERLLRPARVKVAGAPGKENGTAKEAALG"
     gene            complement(1947..3005)
                     /gene="hrcA"
                     /locus_tag="PLB01_17125"
     CDS             complement(1947..3005)
                     /gene="hrcA"
                     /locus_tag="PLB01_17125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQQ79078.1"
                     /translation="MISEFKGLDGRSREVLASVIVAYIESAVPVSSRQLTKAGKFGLS
                     SASLRNAMADLEDLGFLTHPHVSAGRVPTDLGYRAFVKELMTTRLPSAEERARIAEEL
                     APEPFETDRFFQATSRVLFRLTGEVGVVAAPASARFVLDSVHFTRLTERRILVAEVSD
                     AGLVESRLIETRDDFAQAELDAISRRLTVDYAGKTLDEIRALLLEALAEEKVRMDHAL
                     ARTLELGRRAFAEGRPEGDTVFVDGTESLLEKPEFRGDVEALRRMFRVFEEKARLVNL
                     LTDCLSFDGARVVIGSDSVFTGETQTAVVTAPYRKGDRVLGALGVVGPRRMEYERVVP
                     LVEELGRYVSHRLTEGAA"
     gene            3189..3773
                     /locus_tag="PLB01_17130"
                     /pseudo
     CDS             3189..3773
                     /locus_tag="PLB01_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
BASE COUNT          701 a         1723 c         1630 g          763 t
ORIGIN      
        1 tcgaggagga cgaggtcctt gatgtcgccc cgcaggacgc ccgcctggat cgccgcgccg
       61 acggcgacga cctcgtcggg gttgatcgag cggttcgggg gtttcccgaa gatcgtctcg
      121 accatctgct ggaccttcgg cgtgcgcgtc tggccgccga cgaggatgac cgtgtcgacc
      181 tggtccgggg aaaggccggc ggcctcgaga gcgttcttgc aggggccttc cgtccgggcg
      241 atgagttcgg cggcgagcgc ctcgaacgtg tcgcgcgtca ggagcttgtt gatgtgcttc
      301 gggcccgacg cgtcggaagc gatgaacggg agcgccacca tcgtctcgag agtggacgag
      361 agctcgcact tggccttctc ggcggcctcc ttgaggcgct ggagggcgag ccggtcctgc
      421 cggaggtcga tcccgctcga ctgcaggaac tccgcgagga gccagtccat gatcgcgcgg
      481 tcgaagtcct cgccccccag gaacgtgtcc cccgaagtcg agaggacctc gaacatcccg
      541 ttgtcgatcc ggaggatcga gatgtcgaac gtgcccccgc cgaggtcgta gacggcgatc
      601 gtctcctgct tgcggttctt ctcgagcccg tacgcgagcg ccgcggccgt gggctcgttg
      661 atgatgcgca ggacctcgag cccggcgatg cggcccgcgt cgcgcgtcgc ctgccgctgc
      721 gagtcgtcga aatacgccgg gaccgtgatg atcgcttcct tgatctcctc gccgagcgcc
      781 tcctcgcaga actccttgat ctcgcgcagg acgaatgccg cgatctcctc gggcgagtag
      841 tcccggtccc ggacgcggat cttcacgtcg ccgttcgtcg cctcgaccag cgtgtacgaa
      901 aggtgctcgc gcgcgtgctt gacctcggcg tccgtccact tgcggccgat gaggcgtttg
      961 acggcgtaga tcgtctgcgt cgggttcgtg atcgcctggc gtttcgcgat ctggccgacg
     1021 aggcgctcgc cgtcctccgt gaagccgacg atggacggcg tcgtgcgggc gccctcgcgg
     1081 ttggggacga cctggggcga gcccacctcc accacggcga cacaggagtt cgtggtgccg
     1141 aggtcgatcc cgagaacctt agccaagggc ggcctccttc gcggtcccgt tctccttgcc
     1201 cggcgctccg gcgaccttga cgcgcgcggg cctcaggagg cgttcgttca gcatgtatcc
     1261 cttctggaac acctcgagga cggtgcccgg cgccgtgtcg gccgtctctt ccctgacgac
     1321 ggcctcgtgg acgttcgggt cgaacggaag cccgagcgcc ggcacctcga cgaggccgta
     1381 acggcgcagg gactcgccga gctggcgggc gatcatgtcg atgcccgcct ggaagtcctc
     1441 gctcgacgcg tcgccgcggt gcgcgagcgc gcgctcgaag ttgtcgagga cgccgagcag
     1501 gtcgttgaac gcgtcggcga gggcgaactt gaagtactcg gccttgtcct tctcggtgcg
     1561 gcgcttgagg ttgtcgaagt cggccgattt ccggaggagc gcttcgcgca agcgccccgc
     1621 ctcttcctcg gcgcggatcg cgcgctcctt cgtcccctcg agctcgtcgt gggctcgccg
     1681 ggcccggtcg atcgcggctg ccagctcggc ctcggcggct gcgaggcgcg gatccggcgg
     1741 ctgcgcctgc ggcaccggtg ccgggggtcg ctcgggaggc ggatccggaa cccgcgcgga
     1801 cgcggactcg tcctgcttct ggcgctggcg ctcctcgacg gcctcgatcg cgcgctcggc
     1861 ctcctcgagt gccttcgcga cggcgtcccg gctctcggtc tcgaggaaga tgatcccgtc
     1921 gtcctcgtcg cgcctcttgc ggcccgtcac gcggcgccct ccgtgagccg gtggctcacg
     1981 tagcgcccga gctcctcgac gaggggcacg acgcgctcgt actccatccg gcgcgggccg
     2041 acgacgccga gcgcgccgag gacgcggtcg cccttgcggt acggcgcggt gacgacggcc
     2101 gtctgcgtct cgccggtgaa gacgctgtcc gagccgatca cgacccgggc cccgtcgaag
     2161 gacaggcagt ccgtcaggag gttcacgagc cgggccttct cctcgaacac gcggaacatg
     2221 cgccggagcg cttcgacgtc gccccggaac tcgggcttct cgagcaggga ctcggtgccg
     2281 tcgacgaaga cggtgtcacc ctccgggcgg ccttccgcga acgcgcgccg tcccagctcg
     2341 agcgtgcggg cgagcgcatg gtccatccgg accttctcct cggcgagggc ctcgagcagg
     2401 agggcgcgga tctcgtcgag cgtcttgccc gcgtagtcca ccgtgaggcg gcgcgaaatc
     2461 gcgtcgagtt cggcctgcgc gaagtcgtcg cgcgtttcga tcaggcggct ctcgacgagt
     2521 ccggcgtcgg aaacctcggc cacgaggatc cggcgctcgg tgaggcgcgt gaagtgcacc
     2581 gagtcgagga cgaaccgcgc ggacgcgggc gcggccacga ccccgacctc tcccgtgagc
     2641 cggaacagga cacgggaggt cgcctggaag aagcggtccg tctcgaaggg ctcgggggcc
     2701 agctcctcgg cgatccgggc gcgctcctcc gcgctcggaa ggcgcgtcgt catgagctcc
     2761 ttcacgaagg cacggtagcc gaggtccgtc gggacgcgcc cggccgagac gtgggggtgc
     2821 gtgaggaacc cgagatcctc gaggtcggcc atcgcgttgc gcagcgatgc cgacgaaagc
     2881 ccgaactttc cggccttcgt cagctggcgc gaggacaccg gaaccgcgct ttcgatgtac
     2941 gcgacgatca ccgacgcgag cacctcgcgg gatcggccgt ccaacccctt gaattcacta
     3001 atcatgtgcg gcgaattgcg cctccgagaa cgccttcagg ctccaagaac cgtgcgcaca
     3061 gatgataaca aacgggaaag ccctcccggg ggactcctcc cccgggaagc cgcgctccgc
     3121 gccgggcgtc gagaggtccg cggtgtaaga tggaaagtgc tccgtactgg aggtttggcg
     3181 aaggctcgat ggtttttttc aactacgcga cgatgcagat ggccgcgaag atcgtgtact
     3241 acggtcccgg cctctgcggg aagacgacga acctccacgt gatttacggg cgcacggcgc
     3301 ccacgtcgcg cggggagatg gtctgcctcg agaccgagac ggaccggact ctgttcttcg
     3361 acctgctccc gctcgacgtc ggcgtgatcg ggggcttcaa gacgcgtctc cagctctaca
     3421 cggttcccgg ccaggttttc tacaacacga cccgcaagct cgtgctgaag ggcgtcgacg
     3481 gcatcgtctt cgtcgccgac tcgcagcgcc cgatgcggga cccgaacgtc gagagtctca
     3541 agaatctccg cgagaacctc gccgagatcg gcctcaccct cgagagcgtc ccccacgtct
     3601 tccagtacaa caagcgcgac ctccagaaca tcctcacgct cgacgagctc aacgagtcgc
     3661 tcaaccccga cgggtccgtc gagtcgtacg aggcgtctgc cacgaacggc acgggcgtct
     3721 tcgagacgct caaggcgatt tcgaagctca ccctgcgctc cctcaagggc cgcatgacca
     3781 tcgaacagcg ccgtccgacc gtcgtcgtgt cgggcgagac gccggcgccg atcccggccg
     3841 cggcgctcca cgccggagcc gtcggccccc gcacctcggg aatcacggcc gccatcgatt
     3901 cgctgggcga cgagagcggc tccgcggcgc cgcccgcccc ggcccgcgac ccctacgact
     3961 ccacgatctc ccgcctccag cggctccagt ccctccaccg ccagggcgtc gacctgcacg
     4021 ccgcgagcgc ctccggcacg agcctcgcgg ccctcacgcc gcctccatcg gccgcgacgg
     4081 acggaggccc gcaggacgcc ccgttcgagg ccgccaacct gccacccgcg gacgatcccg
     4141 ccgccgaagt gagcttcgcc gagatccagc cgcccccgga cgatccgccg acggactctt
     4201 ccgtgaagca cgtgcgcgtc cgctcgaacg tcgacatcct gtccgagctc gagaagctgc
     4261 ggaaaatcgc gacgcagaaa ccggcctcgc ccgcccagaa ggtccggagc gccgccgacg
     4321 tctcgctcga cgatctgctc ggctcctcgc gcaatcgcaa gaaggacgtc gcgcagagtt
     4381 tcgaggtcct cgttccgcga gacgcactcg cgaagagccg ccgcgtgacc gtggccctgc
     4441 gcttcggcga cgacaccggc gccgcgatcg gcccggagaa gcgcttcgac gtcgagatcg
     4501 ccacgccgca ggatctccag aagctcctgc tctccctcaa gttcaacgtt cgtgccgaat
     4561 gaggggcgcg cgttttctcg ccgtcctcgg agtgctcgtg ttggcggggt gcgcgtccgc
     4621 gccggccgga gatcaggcgg ccgtgccggc tcccccgtcg cccgacaccg ccgcgttcgc
     4681 ggcccgcgcc caggcgacgg acctcttcta ccgcggcaag gccttcgcgc tcgcgggtga
     4741 cgccgactgc gcgcgggatg cgttccacgg cgcgctcgag acgttccgcg cggcggcccg
     4801 ccccgggaat gcgtccg
//
LOCUS       DAOMFY010000296         4802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6327_length_4802_cov_5.039815, whole genome shotgun sequence.
ACCESSION   DAOMFY010000296 DAOMFY010000000
VERSION     DAOMFY010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6327_length_4802_cov_5.039815"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..959)
                     /gene="ftsY"
                     /locus_tag="PLB01_17135"
     CDS             complement(15..959)
                     /gene="ftsY"
                     /locus_tag="PLB01_17135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQQ79079.1"
                     /translation="MASDESPDKPSRTSVFARLKRGLFMTHTELIAKVGGAIKAKFEP
                     DPRALEALEDALLTADVGPATAAELVAAVREEAGRRDAGEADVVRRVLKAEIARRLTV
                     AGPEVAAGRPHVIFMVGVNGTGKTTTTAKLAARAVADGKSVILAAADTFRAAALEQLQ
                     IWADRIGIPLVKLKSGSDPAAVVHDACAAAVAKRTDLLFVDTAGRLHTKHNLMEELSK
                     MRRVAARLVPGAPHEVLLVLDAVTGMNGVSQAREFTKSAGVTGLVLTKMDGTAKGGVI
                     LAIVRELGLPVKYVGVGETADDLLDFDPDAFASALVDD"
     gene            1117..1848
                     /locus_tag="PLB01_17140"
     CDS             1117..1848
                     /locus_tag="PLB01_17140"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HQQ79080.1"
                     /translation="MTVTTPATDGPRPLPRHVAVIGAAGGLGQGILSVCRAEGVRFTA
                     IVRSRPERIKDVPSGSRVAVVPSLADRAALKDAFSGAGAVLTAMGVTAATKERSALLS
                     ANMVPVEESMLAAGVDRIIVINTLLTSQPGRPASLAMHFFGWMPGTIGRGARELQAVA
                     DALGRGAFLSLRWTLVRGGLNSRGRDERPVASGDWDGALNSWSPVSYRAMGRWMLEEA
                     AADEFVRAAPLVSRRRRERTVRTSA"
     gene            complement(1858..2208)
                     /locus_tag="PLB01_17145"
     CDS             complement(1858..2208)
                     /locus_tag="PLB01_17145"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HQQ79081.1"
                     /translation="MSFETILSSLLESPGALGAAFLDPQGEVIARAGDEAATEILGAY
                     QSVWLAELGRAAERSGLGAVSGLALEFDGRKVLTAPVKAGFFLLVVLSPDGRPSVVKA
                     GMDAARERLASEVS"
     gene            complement(2205..3014)
                     /locus_tag="PLB01_17150"
     CDS             complement(2205..3014)
                     /locus_tag="PLB01_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ79082.1"
                     /translation="MRGGPPALRAASAGVALLLAAVAASGCGPQMAAPPKENPVLADL
                     VDYRSALTMLREGRADEAIALLQRARQASPRDPHVPNAMGLALLYKKDYPGALRAFSD
                     ALSLDDTFVEARNNRGVAYFQAGRWDEAETDFQAVLDGPPTPEKVNAHYNLGLLQDKR
                     GRYRDAEREYSLAIADSPDNTRAYRARGLARMKLEQTSSALEDFLVVLKDEPKDPVAN
                     YQAAICLLASDRRDLARKFMQRAVEASPESDEGRKARRWLENEPRPPEGKP"
     gene            complement(3011..4732)
                     /locus_tag="PLB01_17155"
     CDS             complement(3011..4732)
                     /locus_tag="PLB01_17155"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQQ79083.1"
                     /translation="MGVSRVPRLRIVTALLTALVVVSIVPLVLLTLNVTRINRDALET
                     AEKKYLKNSSVGLAEKFETAITNAQTQLRKIADGLRLASSMSPDPFYYLSKKELLREY
                     ADADASFLVLRALDRDGVGAVAESRSLDIDIQRALRQAFAAAVRGETYVGEPLFTGVL
                     PEGGLVLAVPVRPADGQEPIGAVEALLSLAPLRAKLEEEGRSTGVLAYVVDRSGTLVL
                     ASDPAAFPSRDIHQLDLVSEFIAHPVRMTKSYSRGGRRVLGTLAAMTTPDWGVVVEKE
                     EAQAYASVAQTQKASLLLAVFSLAFAALVALFAARMLAHPLEELVEKVRSIAGGDFRQ
                     RVSVRGVRELAELSETFNSMSDSLEKSIEKLKLAAKENQELFINSVRTLAAAIDAKDP
                     YTRGHSERVARYAVVLARHMDLTPEEVRIIRLSALLHDVGKIGIDDRILRKPTALTNE
                     EFEIMKTHPIKGALIMGQIPQLKEVIPGIKHHHEKWSGGGYPDGLKGEGIPLVARVVA
                     VADTFDAMTTTRPYQKAMPLNYVIRQIKSFAGKSFDPAVIAALDKAWSSGDFEFLGEE
                     VPLKASA"
BASE COUNT          690 a         1628 c         1720 g          764 t
ORIGIN      
        1 cggtcggctt cgagtcagtc atcgacgagc gcggacgcga acgcgtcggg gtcgaaatcg
       61 aggaggtcgt cggccgtctc gccgacgccg acgtacttca cgggcaggcc gagctcccgg
      121 acgatcgcga ggatgacgcc gcccttggcc gtcccgtcca tcttcgtgag gacgagaccg
      181 gtcacgccgg ccgacttcgt gaactcccgg gcctgggaaa cgccgttcat gcccgtcacc
      241 gcgtcgagga cgaggaggac ctcgtgcggc gctccgggga cgaggcgcgc ggcgacgcgc
      301 cgcatcttcg agagctcctc catgaggttg tgcttcgtgt gcaggcggcc cgccgtgtcc
      361 acgaagagga ggtcggtcct cttcgcgacc gcagcggcgc acgcgtcgtg gacgacggcg
      421 gccggatcgg agccggactt gagtttcacg agcgggatcc cgatgcggtc ggcccagatc
      481 tggagctgct cgagggcggc cgcgcggaac gtgtcggcgg ccgcgaggat tacggatttt
      541 ccgtccgcga cggcgcgcgc ggcgagcttc gccgtggtcg tggtcttccc ggtcccgttc
      601 acgccgacca tgaagatcac gtgcggccgc ccggccgcca cctcgggccc cgcgaccgtg
      661 aggcgccgcg cgatctccgc cttcaggacg cggcgcacga cgtcggcttc cccggcgtcg
      721 cgccgccccg cttcctcccg gaccgcggcg acgagctcgg cggccgtcgc ggggccgacg
      781 tccgccgtca ggagggcgtc ctcgagggct tccagcgccc tcgggtcggg ctcgaacttc
      841 gccttgatcg ccccgcccac cttcgcgatg agctcggtgt gggtcatgaa cagcccgcgc
      901 ttcaggcggg cgaacacgga ggtgcgggag ggtttgtcgg ggctctcgtc ggacgccatg
      961 ggcccgcgag actagcagga agggccggat ttccgagcgg tgaacgagcg cctcgcatcg
     1021 caggtctcca gcgatcggct gcgctccttc ccttccgccg ctggacccga tatcgtcggt
     1081 ggctctggcg gtccctcgtc agattcgagt gatcggatga ccgtgacgac accagcgacc
     1141 gacggccctc gcccactccc tcgtcacgtc gcagtcatcg gcgccgcagg ggggctcggc
     1201 cagggcatcc tcagcgtctg ccgggcggag ggagtcaggt tcaccgccat cgtccgttcg
     1261 cgcccggagc gcatcaagga cgtgccaagt ggttcccgcg ttgcggttgt cccgtctctg
     1321 gccgatcggg ccgctctcaa ggacgcgttt tccggcgcag gcgccgtgct gaccgccatg
     1381 ggagtcacgg cggcaacgaa ggagcgctcg gccctgctct ccgcgaacat ggtgccggtc
     1441 gaagagtcga tgttggccgc gggcgtcgat cgcatcatcg tcatcaatac gctgctcacg
     1501 tcgcaaccgg ggcggcctgc gagcctcgcg atgcacttct tcgggtggat gcccggcacg
     1561 atcggccgcg gtgctcgtga gcttcaagcg gtggcggatg cgctcggccg cggagcgttc
     1621 ctctcgcttc gctggactct cgtgagaggc gggctgaact ctcggggcag ggacgagcgc
     1681 cctgtcgctt cgggggattg ggacggggcc ctcaactcct ggtcgcccgt gtcgtatcga
     1741 gcgatgggcc gatggatgct tgaagaagcc gcagccgatg aattcgtccg cgccgcgcca
     1801 cttgtgtcgc ggcgccgtcg cgagagaaca gtccgaacgt cggcctagcc gcgggactca
     1861 ggagacttcg gacgcgaggc gttcgcgggc cgcgtccata ccggccttca cgaccgacgg
     1921 gcggccgtcg ggcgagagga cgacgagcag gaagaatccg gccttcacgg gcgcggtgag
     1981 gacctttcgt ccgtcgaatt ccagcgcgag gcccgagact gcgccgaggc ccgagcgctc
     2041 ggcagcccgc cccagctcgg ccagccagac cgactggtac gcccccagga tctccgtcgc
     2101 ggcctcgtcc ccggcgcgcg cgatgacctc gccctgcggg tcgaggaacg cggcgccgag
     2161 ggcgcccggc gactcgagaa ggctcgacag gatcgtctcg aagctcacgg cttcccctcc
     2221 ggcggccggg gctcgttctc gagccagcgt ctcgccttcc ggccttcatc gctctcgggc
     2281 gacgcctcca ccgcccgctg catgaacttg cgggcaaggt cccgccggtc gctcgcgagc
     2341 aggcagatcg ccgcctgata attcgccacg ggatccttgg gctcgtcctt caggacgacg
     2401 aggaaatcct cgagcgcgct cgacgtctgc tcgagcttca tgcgtgcgag gccgcgcgcc
     2461 cggtaggccc gggtgttgtc cggagagtcc gcgatcgcga gggagtactc gcgctcggcg
     2521 tcccggtaac gccccctctt gtcctgaagg agcccgaggt tgtagtgcgc gttgaccttc
     2581 tcgggcgtcg ggggcccgtc gaggacggcc tgaaagtcgg tctcggcctc gtcccacctg
     2641 ccggcctgga aatacgcgac gccgcggttg ttccgggcct cgacgaaggt gtcgtcgagc
     2701 gagagcgcgt ccgaaaaggc cctcagggcc cccggatagt ccttcttgta gagcagcgcg
     2761 aggcccatcg cgttcggcac gtgggggtcc cggggcgagg cctgacgggc ccgctgcagg
     2821 agcgcgatcg cttcgtccgc gcgcccctcg cgcagcatcg tgagggcgct gcggtagtcc
     2881 acgaggtccg cgaggacggg gttttccttc ggcggcgcgg ccatctgagg gccgcagccc
     2941 gacgcggcga cggccgcgag cagcagagcg accccggccg aggccgctcg aagggccgga
     3001 ggaccgcccc tcacgcggac gccttcagcg ggacttcctc gccgaggaac tcgaagtcgc
     3061 ccgacgacca ggccttgtcg agtgcggcga tgacggccgg gtcgaacgac ttgcccgcga
     3121 aggacttgat ctggcggatc acgtagttga gcggcatggc cttctggtag ggccgcgtcg
     3181 tcgtcatcgc gtcgaacgtg tcggcgaccg ccacgacgcg ggccacgagc gggatgccct
     3241 ctcccttgag gccgtccgga tacccgccgc ccgaccactt ctcatggtgg tgcttgatgc
     3301 ccgggatgac ctccttgagc tgggggatct ggcccatgat gagcgcgccc ttgatcgggt
     3361 gcgtcttcat gatctcgaac tcctcgttcg tgagcgccgt gggcttgcgc aggatgcggt
     3421 cgtcgatccc gatcttgccg acgtcgtgga ggagggccga gagccggatg atgcggacct
     3481 cctcgggggt gaggtccatg tgccgggcga ggacgacggc gtagcgcgcg acgcgctcgg
     3541 agtggccgcg cgtgtacggg tctttcgcgt cgatcgcggc cgcaagcgtc cgcaccgagt
     3601 tgatgaacag ctcctggttc tccttggcgg cgagcttgag cttctcgatg gacttctcga
     3661 gagagtccga catcgagttg aacgtctccg agagctcggc gagctctcgc acgccgcgca
     3721 cggacacgcg ctggcggaag tcgccgcccg cgatggaccg gaccttctcg acgagctcct
     3781 cgagcgggtg cgcgagcatc cgcgccgcga agagcgcgac gagcgcggcg aacgcgagag
     3841 agaagacggc gaggaggagc gacgccttct gcgtctgcgc gacggacgcg taggcctgcg
     3901 cctcttcctt ctcgacgacg acgccccagt cgggcgtcgt catggcggcg agcgtcccga
     3961 gaacgcggcg gcccccgcgc gagtacgact tcgtcatccg gaccgggtgc gcgatgaatt
     4021 cgctcacgag gtccagctgg tggatgtccc gcgaggggaa cgcggccggg tcgctcgcga
     4081 ggacgagcgt gcccgagcgg tccacgacgt aggcgaggac gcccgtggat ctcccctcct
     4141 cctccagttt cgcccggagc ggcgcgaggg agagcagcgc ttcgacggcg ccgatcggct
     4201 cctggccgtc ggccggtcgg acgggtacgg cgaggacgag gccaccctcg gggagaaccc
     4261 ccgtgaacag gggctcgccg acgtaggtct ctccccgcac cgcggccgcg aaggcctggc
     4321 ggagggcgcg ctggatgtcg atgtcgagcg accgggactc cgccacggcg ccgacgccgt
     4381 cgcggtcgag agcccggagg acgaggaagc tcgcgtccgc gtcggcgtat tcgcggagga
     4441 gctccttctt cgacaggtag tagaagggat ccggcgacat cgagctcgcg aggcgcaggc
     4501 cgtcggcgat cttgcggagc tgggtctggg cgttcgtgat ggccgtctcg aacttctccg
     4561 cgagcccgac ggagctgttc ttcaggtact tcttctcggc cgtttcgagc gcgtcgcggt
     4621 tgatccgcgt cacgttcagc gtcaggagga cgagagggac gatcgagacc acgacgagcg
     4681 cggtgagcag agccgtcacg atccgcagac gcggaactct cgatacaccc atccagattc
     4741 aattgtaccg cgccagcgct tcctcgagaa gaacgggttg ctccatgagc ccgaagcagg
     4801 cc
//
LOCUS       DAOMFY010000297         4748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6427_length_4748_cov_5.418709, whole genome shotgun sequence.
ACCESSION   DAOMFY010000297 DAOMFY010000000
VERSION     DAOMFY010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6427_length_4748_cov_5.418709"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLB01_17160"
     CDS             <1..720
                     /locus_tag="PLB01_17160"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQQ79084.1"
                     /translation="FGDWKAAGPAPKRPAVPAAYAPAERGLRLYLVDRPDAAQTSVRF
                     LAPAPRFADPSRVPLRLLGTVLGGSFTSRLNQNLREKHGYTYGAGARFQQGPVLGTFS
                     AGAEITAKDTGAAIREFLAEFDRLSKGDVTDEEAGKARGLVKNGVVGAFSGLSGIVGT
                     AASYVEIGVPFETLGEDLAAMAKTNTAALNAQAKTALPLDRAVLVLVGDKATILPQLK
                     EAGLPTPVEVDSWGEKKGKKE"
     gene            complement(837..989)
                     /gene="rpmG"
                     /locus_tag="PLB01_17165"
     CDS             complement(837..989)
                     /gene="rpmG"
                     /locus_tag="PLB01_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQQ79085.1"
                     /translation="MREKVKLVSTAGTGYFYTITKNKKIHQDKMEVKKYDPKIRKHVP
                     FKESKI"
     gene            complement(993..1229)
                     /gene="rpmB"
                     /locus_tag="PLB01_17170"
     CDS             complement(993..1229)
                     /gene="rpmB"
                     /locus_tag="PLB01_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQQ79086.1"
                     /translation="MSGICQVTGKRATSGNKVSHANNKTKRLFKPNLRSQRFWVESEG
                     RWVRLRVSTAAMRTIDKKGIDAVVAEMRREGKKV"
     gene            1337..2083
                     /locus_tag="PLB01_17175"
     CDS             1337..2083
                     /locus_tag="PLB01_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79087.1"
                     /translation="MSRARILALLLLGAAGPLRAQDPFEIQVYEYETVPKGRWNLETH
                     VNYVSKGTTAWEGPMAPTEGQFHTTFELTRGITDNFELAGYLLFGSYTGGSFQWAGGR
                     IRPRVSAPKEWNWPIGVSLSVEIGFPVKVFEENGTTLEIRPILEKKLGDWQIDFNPVV
                     GTALSGPGRGEWDFEPGLRVAYEASKTLDLSVEYYGAIPFSGGESVHQIYPGADVHLS
                     EVVVLNVGAGWGLTDAGNRFVWKMRLGVMF"
     gene            complement(2080..2841)
                     /gene="gpmA"
                     /locus_tag="PLB01_17180"
     CDS             complement(2080..2841)
                     /gene="gpmA"
                     /locus_tag="PLB01_17180"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HQQ79088.1"
                     /translation="MSSAPLPNKLVLVRHGESTWNLENRFTGWVDVGLSPKGVEEAHA
                     AGKLLKAEGFAFDRCYTSLLKRAILTLQIVLEELDQIWLPVERSWRLNERHYGALQGL
                     NKKETAQKYGDEQLFKWRRGYAITPPPLDANDPGHPRFDPRYAGMPPEILPATESLAI
                     VVDRFLPYWYDAIVPRLARGEQVLVAAHGNSLRALVKHLDGISDEDIPELNIPTGIPL
                     VYTLDGSFRKVDSRYLGDPEAAKKAAEAVAKQAKT"
     gene            complement(2864..3664)
                     /locus_tag="PLB01_17185"
     CDS             complement(2864..3664)
                     /locus_tag="PLB01_17185"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HQQ79089.1"
                     /translation="MIEFFSYPFFQRALLAGALAALLCGALSFFVVLRRLAFVGSGVA
                     HAAFGGVAVATLFGFPPLAGGLVAALLVAGATARAQGSSVTEDSAVGVFTVAAMALGV
                     VALGFVKTNVDLFGLMFGNILTVAPADLAVLGVAAAAVLAALAAFFRPLLLASVDEEG
                     AEVAGVNTGAMRLLVLVLVGVAVVVALRVVGILLVSALLVLPGAAARALAARWPALLA
                     GSITVALVSVVGGLLLSVAVDVAPGAAIILVGAAIFVAALVVGRTRRR"
     gene            complement(3661..4293)
                     /locus_tag="PLB01_17190"
     CDS             complement(3661..4293)
                     /locus_tag="PLB01_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ79090.1"
                     /translation="MTLGSRQVLKDVSFEIPAGERTAFVGPNGGGKTTIVRALLGIIR
                     PGAGRVVFFEGDEKKEIPRPRIGYLSQRSTLALDAPLSAEDLVAFSLSPEPGFRHRSD
                     ASVEARAALLRSGLSKELFDVPVGFLSGGQRQRVLLARAIAGAPPLLVLDEADTALDT
                     AGLATLRRLVTEEVAAGRTVVYVTHDSDAMGGADAVFRVDGVVVEEPVDA"
     gene            complement(4323..>4748)
                     /locus_tag="PLB01_17195"
     CDS             complement(4323..>4748)
                     /locus_tag="PLB01_17195"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HQQ79091.1"
                     /translation="DPSGVEGYRARAAEAGRSLAVLDREVAAALAPVRGRAVVSTHNS
                     WAYFLDDYGLVNGGSIEPVPGREPSSRQLRALIDLVRERRIPALFTEPQFPPSAARVV
                     AGDAGVRVVLADPIGGVPGRETYAELLRFDARAFREGLR"
BASE COUNT          798 a         1627 c         1655 g          668 t
ORIGIN      
        1 ttcggcgact ggaaggcggc cggcccggcg ccgaagcggc ccgcggtgcc cgcggcctac
       61 gcacccgcgg agaggggcct gcgcctctac ctcgtggacc ggcccgacgc ggcccagacg
      121 agcgtgcgtt tcctcgcgcc cgccccgcgc ttcgcggacc cgtcacgggt cccgctgcgc
      181 ctgctcggca cagtcctcgg cggcagcttc acgagccgcc tgaaccagaa ccttcgcgag
      241 aagcacggtt acacatacgg cgccggcgcg cgcttccagc aggggcccgt cctcggcacg
      301 ttctcggccg gggccgagat caccgccaag gacaccggcg ccgcgatcag ggagtttctc
      361 gccgagttcg accgtctctc gaagggcgac gtcacggacg aggaggccgg caaggcgcgc
      421 ggcctcgtga agaacggggt cgtcggcgcg ttctccggcc tctcggggat cgtgggaacc
      481 gcggcgagct acgtcgagat cggcgttccg ttcgagactc tcggcgagga cctcgccgcc
      541 atggcgaaga cgaacaccgc cgccctgaac gctcaggcga agacggcgtt gcctctcgac
      601 cgcgcggtcc tcgtcctcgt gggcgacaag gcgacgatcc tcccccagct caaggaggcg
      661 ggcctgccga caccggtcga ggtcgacagt tggggggaga agaaagggaa aaaagaatag
      721 gagttcctag gaggtgaaga gggcggcgcc ggcttcccgt aggtcgccgc ctcaatgcaa
      781 agggcgccgg cttcccggcg ccctactaac ctctgagaaa atccgaaaat cttctcttag
      841 atcttcgatt ccttgaacgg gacgtgcttg cggatcttgg ggtcgtactt cttcacttcc
      901 atcttgtcct gatggatctt cttgttcttc gtgatcgtgt agaagtaccc ggtgcccgcc
      961 gtggagacga gcttgacctt ctcgcgcatc ggctagacct tcttcccttc gcggcgcatc
     1021 tcggccacga cggcgtcgat acccttcttg tcgatggtgc gcatggcggc cgtcgacacg
     1081 cggagccgca cccagcggcc ttcgctctcc acccagaaac gctgcgagcg caggttcggc
     1141 ttgaagaggc gcttcgtctt gttgttggcg tgcgacacct tgttgccaga cgtcgcccgc
     1201 ttccccgtga cctgacaaat tccggacatc tcgaaactcc ttcgacccag agccgatgag
     1261 cttaccagaa gatggagaat tcggcaaccc actgaggacg agagggttgc gggcggaaga
     1321 gctagatttg ccgcacatga gtcgcgcccg catcctcgcc ctcctgctcc tcggcgctgc
     1381 cggccccctc cgcgcccagg acccgttcga gatccaggtc tacgaatacg agaccgtccc
     1441 gaagggccgg tggaacctcg agacgcatgt gaactacgtc tccaagggga cgacggcctg
     1501 ggaagggccg atggctccca cggagggaca gttccacacg acgttcgagc tgacgcgcgg
     1561 gatcaccgac aacttcgagc tcgccggcta cctcctcttc ggctcgtaca cgggcggctc
     1621 gttccagtgg gcgggcgggc gcatccggcc gcgcgtcagc gcgccgaagg aatggaactg
     1681 gccgatcggc gtctcgctca gcgtcgagat cggcttcccg gtcaaggtct tcgaggagaa
     1741 cggaacgacc ctcgagatcc ggccgatcct cgagaagaag ctcggggact ggcagatcga
     1801 cttcaacccc gtcgtcggga cggcgctgag cgggccgggc aggggcgagt gggacttcga
     1861 gcccggcctg cgcgtcgcgt acgaggcctc gaagacgctg gacctgtccg tcgagtacta
     1921 cggcgcgatc ccgttctcgg gcggcgagtc ggtccaccag atctatcccg gggccgacgt
     1981 ccatctctcc gaagtcgtcg tcctgaacgt gggagccggc tgggggctca cggacgcggg
     2041 gaaccggttc gtctggaaga tgcgtctcgg cgtgatgttc taggtcttcg cctgcttcgc
     2101 cacggcctcg gcggccttct tcgcggcctc gggatcgccg aggtaccggc tgtccacctt
     2161 ccggaacgag ccgtcgagcg tgtagacgag cgggatgccc gtcgggatgt tgagctcggg
     2221 aatgtcctcg tcggaaatgc cgtccagatg cttcacgagg gcgcgcaggc tgttgccgtg
     2281 cgccgcgacg agcacctgct cgccgcgagc gagccgcggc acgatggcgt cgtaccagta
     2341 cgggaggaaa cggtccacga cgatcgcgag gctctcggtc gccgggagga tctccggcgg
     2401 cattcccgcg taacgcgggt cgaaccgcgg gtgcccggga tcgttcgcat cgagcggcgg
     2461 cggggtgatc gcatagccgc ggcgccactt gaagagctgc tcgtcgccgt acttctgcgc
     2521 cgtctccttc ttgttgaggc cctggagcgc cccgtagtgc cgctcgttga ggcgccacga
     2581 gcgctcgacg gggagccaga tctggtcgag ttcctcgagg acgatctgga gggtcaggat
     2641 cgcgcgcttg aggagggacg tgtaacagcg gtcgaacgca aagccctcgg ccttgaggag
     2701 tttcccggcc gcgtgcgctt cttcgacgcc tttcggagag aggccgacgt ccacccagcc
     2761 cgtgaagcgg ttctcgaggt tccaggtgct ctcgccgtgg cggacgagaa cgagcttgtt
     2821 cggaagcggt gcgctgctca tggccgaagc ataaaccggg cggtcaccgg cggcgggtcc
     2881 gcccgaccac gagcgccgcg acgaagatcg ccgcgcccac gaggatgatc gccgcgcccg
     2941 gtgcgacgtc gacggccacc gacaggagca gcccccccac caccgaaacg agcgcgacgg
     3001 tgatcgagcc cgcgaggagg gccggccagc gcgccgcgag agcccgcgcc gccgcaccgg
     3061 gcaggacgag gagcgccgag acgagcagga tgccgacgac cctgagcgcg acgacgaccg
     3121 cgaccccgac gaggacgagg acgagaagcc gcatcgcgcc cgtgttcacg cccgcgacct
     3181 cggcgccctc ctcgtccacg gacgcgagga gcagcggccg gaagaaggcc gcgagcgcgg
     3241 cgaggaccgc cgccgccgcg acgccgagga cggcgaggtc ggcgggcgcc acggtcagga
     3301 tgtttccgaa catgaggccg aagaggtcga cgttcgtctt cacgaagccg agcgcgacga
     3361 cgccgagcgc cattgcggcg accgtgaaga cgccgacagc ggagtcctcg gtcacggacg
     3421 atccctgggc ccgcgccgtc gcccctgcga cgaggagggc ggccacgaga ccgcccgcca
     3481 gcggcgggaa gccgaagagc gtcgcgaccg cgacgccgcc gaacgcggca tgcgcgacgc
     3541 ccgacccgac gaaggccagg cgccggagga cgacgaagaa cgacaaggct ccgcacagga
     3601 gagccgcgag cgcgccggcg aggagcgcac gctggaagaa cgggtacgag aagaactcga
     3661 tcatgcgtcc accggctcct cgacgacgac cccgtccacc cggaagacgg cgtccgcccc
     3721 gcccatcgcg tcgctgtcgt gagtcacgta gacgacggtg cgccccgcgg cgacctcttc
     3781 ggtgacgagc cgccgcagcg tggcgaggcc cgccgtgtcg agcgccgtgt cggcctcgtc
     3841 gaggacgagg agcgggggcg cgcccgcgat cgcccgggcg aggaggacgc gctggcgctg
     3901 cccgcccgag aggaagccga cgggaacgtc gaagagttcc ttcgacaggc ccgacctcag
     3961 gagcgccgcg cgcgcttcga ccgaagcgtc ggagcgatgg cgaaatccgg gctccggcga
     4021 tagagaaaat gcgacgagat cctcagccga gagcggcgcg tcgagcgcga gcgtcgagcg
     4081 ctgagagagg tacccgatgc gagggcgcgg gatctccttc ttctcgtcac cttcgaagaa
     4141 gacaactcga cccgcgcctg gccggatgat tccgaggagg gcccggacaa tcgtcgtctt
     4201 cccgcctccg ttcggtccca cgaacgccgt gcgctccccg gccgggatct cgaaggaaac
     4261 gtccttcagg acctgccggg agcccagcgt cacggagact cctccgaacc ggacgccgat
     4321 gctcaccgga ggccctcccg aaacgcgcgc gcatcgaagc gcagaagctc cgcgtacgtc
     4381 tcgcggccgg ggacgccgcc gatcgggtcg gccaggacga cgcgtactcc ggcgtcgccc
     4441 gccaccacgc gcgccgccga cggcgggaac tggggctcgg tgaagagcgc cgggatgcgc
     4501 cgttcgcgca cgaggtcgat gagcgcgcgg agctggcgcg acgacggctc gcgccccggc
     4561 acgggctcga tcgacccgcc gttcacgagg ccgtagtcgt cgaggaaata ggcccacgaa
     4621 ttgtgcgtgg acacgaccgc tctgccgcgc acgggcgcga gcgccgcggc gacctcccgg
     4681 tcgagcacgg caagcgaacg gcccgcttcg gcggcgcgcg cgcggtagcc ctccacgccg
     4741 gaagggtc
//
LOCUS       DAOMFY010000298         4740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6446_length_4740_cov_7.182924, whole genome shotgun sequence.
ACCESSION   DAOMFY010000298 DAOMFY010000000
VERSION     DAOMFY010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6446_length_4740_cov_7.182924"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..498)
                     /locus_tag="PLB01_17200"
     CDS             complement(10..498)
                     /locus_tag="PLB01_17200"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HQQ79092.1"
                     /translation="MARKQLWGGRFSEPPAQALKAFNDSLAFDRALLAEDVQGSIGWA
                     QALGAAGILTAAEVKKLVSALEEVAAEAARAGVPADADDEDVHSFVEGRLAAKVGPLS
                     GKLHTGRSRNDQVATDFRLYVKDALAEGAVAARALALALARRAEAEAATPMPGGSRPA
                     DR"
     gene            complement(501..1733)
                     /locus_tag="PLB01_17205"
     CDS             complement(501..1733)
                     /locus_tag="PLB01_17205"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQQ79093.1"
                     /translation="MKPKIKKACLAYSGGLDTSVIIPWLKENYGCEVVAVAVDVGQAE
                     ETSGLAEKAKKTGASEFHLIDAKEEFAKDFLFPVLKAGAVYEHDYLLGTSTARPLIAR
                     KQVEIALATGCDALSHGCTGKGNDQVRFELAYMALAPDLAIIAPWREWEIRSREDAID
                     YAAARGIPVPVTKKDPYSRDRNLWHISHEGGPLEEPGFEPEESMFKLTTDPRKAPDEP
                     ERVVISFEAGLPVAVNGKKLAPVALIEALNAIAGRHGVGRVDLVENRLIGIKSRGVYE
                     TPGGTLLVQALRALETLTLDRDSVHEKEKLAARYAELVYFGQWFSPLREALDAFMNAL
                     MPTVTGDVTLKLFKGASSVVGRTSPFALYSTSLASFDMRGYTPADAAGFIRLFGLPTK
                     GRLHLKPGTYQAVVGGEG"
     gene            complement(1730..2950)
                     /locus_tag="PLB01_17210"
     CDS             complement(1730..2950)
                     /locus_tag="PLB01_17210"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HQQ79094.1"
                     /translation="MQTSAEDTAAVEARDAAYVLGTYKRTSFHPRSGKGAKLVDADGK
                     VYWDLLAGIAVNALGYRHPRLVKALKDEAGRVLHVSNLFYQPAQGLLAERLVALSGFP
                     KVFFCNTGTEATEAAVKFTRLATPGRSRMIALEGGFHGRTMGALALTGNEGYRKPFEP
                     LVGTTVFLPPNDVAALNAAVTPDTSAIFLEPILGEGGIIPLTPEYVAAARAAADRVGA
                     SLVLDEVQSGLGRTGHLFVFRELGVTPDLVTLAKPLGGGLPLGAVLVGPKIAALVKAG
                     QHGTTFGGNPVACRLGLETLDEIVSGGLLPKIQETGAWFAKKLKIAKRKSKGAIVDVR
                     GRGLMWGIELDRDAAEIQKRLLGKGFVVGTSRSNVLRLLPPFVIPRPALKAFLAALDE
                     VLAETTAPSKEKSS"
     gene            complement(2934..3734)
                     /gene="argB"
                     /locus_tag="PLB01_17215"
     CDS             complement(2934..3734)
                     /gene="argB"
                     /locus_tag="PLB01_17215"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQQ79095.1"
                     /translation="MSANRPGRPIVVKLGGSLLEDAVPRAAALAAIAASWTSGENVVV
                     VHGGGKRIDASLQALKIPKKTYRGLRITDPETLDVVVSILSGLVNKSLVAELRSLGVT
                     AAGVSGADGETLWAEFHPRLDGVDFGYVGRVTFSDPRLIRAILGAELLPVVASVALGR
                     EGTLLNVNADSAASALAVALQAERLVFLTDVEGVLDGNGKVIEGLGPDATWELLNSGV
                     VAGGMRPKLVACLEALKAGVSQVVIAGPGRHASALAEGIGGTRIANIG"
     gene            complement(3731..>4740)
                     /gene="argC"
                     /locus_tag="PLB01_17220"
     CDS             complement(3731..>4740)
                     /gene="argC"
                     /locus_tag="PLB01_17220"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQQ79096.1"
                     /translation="VVGATGYSGGELCAILARHPRASVSSAFSSGRKDAKPVPFSRLH
                     PSLGGARGPSADVFSLEALAAGRPDVVFLATPNETSAEVAAEILALGARVVDVSGAFR
                     LRNASDYPKWYGFDHPAPALLAEAVYGLTEWCGPELSGARLVANPGCYPTSVLLALKP
                     VLELLEPGASVVADSKSGVSGAGKKSDLAFSFSELAGNFKAYGVGSHRHEPEMRQELG
                     LSADAPFVFVPHLLPVVRGILSTLHVTFRAGVGPADVAGAYARYADAPFVSVRPAGEL
                     PDLASVVGTPRAEIGFAILSGGRRGVIVSVIDNLLKGAASQAVQNMNRVFGFPETEGL
                     A"
BASE COUNT          693 a         1660 c         1723 g          664 t
ORIGIN      
        1 cgggcacatt catcggtcag cgggcctcga cccgccgggc atgggcgtcg cggcctcggc
       61 ctcggcccgc ctcgcgagcg cgagggcgag cgcccgcgcc gcgacggcgc cctcggcgag
      121 cgcgtccttg acgtagagcc ggaagtccgt cgcgacctgg tcgttgcgcg agcggcccgt
      181 gtggagcttc cccgagagcg gcccgacctt ggcggcgagc cggccctcga cgaacgagtg
      241 gacgtcctcg tcgtcggcgt cggccggaac gcccgcccgc gcggcctcgg ccgcgacctc
      301 ctcgagggct gaaacgagct tcttcacctc ggccgcggtg aggatcccgg ccgcgccgag
      361 cgcctgcgcc cagccgatcg agccctgcac gtcctcggcg aggagcgccc ggtcgaaggc
      421 gagggagtcg ttgaacgcct tcagggcctg cgcgggcggc tcggagaacc ggccgcccca
      481 gagctgcttg cgggccaagg tcagccttcc ccgccgacga cggcctgata cgtgcccggc
      541 ttgaggtgga ggcggccctt cgtcggcagg ccgaagagcc ggatgaaccc tgcggcgtcc
      601 gccggcgtgt agccgcgcat gtcgaaggac gcgaggctcg tcgagtagag cgcgaacggc
      661 gaggtgcgcc cgacgacgga cgaggcgccc ttgaagagct tcagcgtgac gtcgcccgtc
      721 acggtcggca tgagggcgtt catgaacgcg tccagcgcct cgcgcagggg cgagaaccac
      781 tggccgaagt agacgagctc ggcgtagcgg gccgcgagct tctccttctc gtgcacgctg
      841 tcgcggtcga gcgtgagcgt ctcgagcgcg cggagcgcct gcacgaggag cgtgccgccc
      901 ggcgtctcgt acacgccgcg cgacttgatc ccgatgaggc ggttctcgac gaggtccacg
      961 cggccgacgc cgtgccggcc cgcgatcgcg ttgagcgcct cgatgagcgc gacgggcgcg
     1021 agcttcttcc cgttgacggc gacggggagg cccgcctcga acgagatcac gacgcgctcg
     1081 ggctcgtccg gcgcctttcg cggatccgtc gtgagcttga acatcgactc ctcgggctcg
     1141 aagcccggct cctcgagggg cccgccctcg tgcgagatgt gccagaggtt gcggtcacgc
     1201 gagtaggggt ccttcttcgt gaccggcacg gggatgccgc gcgccgcggc gtagtcgatc
     1261 gcgtcctcgc gcgagcggat ctcccattcg cgccacggcg cgatgatcgc gaggtccggc
     1321 gcgagcgcca tgtaggcgag ctcgaagcgg acctggtcgt tgcccttgcc cgtgcatccg
     1381 tgggaaagcg cgtcgcagcc cgtcgcgagc gcgatctcga cctgcttgcg ggcgatgagg
     1441 ggccgcgccg tcgaggtccc gaggaggtag tcgtgctcgt acacggcgcc ggccttgagg
     1501 accgggaaga ggaagtcctt cgcgaactcc tccttggcgt cgatcaggtg gaactccgac
     1561 gcgccggtct tcttcgcctt ctcggcgagg ccggacgtct cctcggcctg gcccacgtcg
     1621 acggcgaccg cgacgacctc gcagccgtag ttctccttga gccacgggat gatgacggac
     1681 gtgtcgaggc cgcccgagta cgcgaggcag gccttcttga tcttcggctt cacgagcttt
     1741 tctccttgct gggtgcggtg gtctcggcga ggacctcgtc gagcgcggcg agaaacgcct
     1801 tcagcgccgg ccgcggaatc acgaagggag gaaggagtcg gaggacgttc gagcgggacg
     1861 tcccgacgac gaaccccttt ccaagaagtc ttttctggat ctcagcggcg tcgcggtcga
     1921 gctcgatgcc ccacatcagc ccgcggccgc gcacgtccac gatcgcgccc ttgctcttgc
     1981 gctttgcgat cttgagcttc ttcgcgaacc acgcgcccgt ctcctgaatc ttcgggagca
     2041 gcccgccgct cacgatctcg tccagcgtct cgaggccgag gcggcaggcg acgggattgc
     2101 cgccgaacgt ggtgccgtgc tgcccggcct tcacgagcgc cgcaatcttc ggcccgacga
     2161 ggaccgcccc gagcgggagg ccgccgccga gcggcttggc gagcgtgacg aggtcgggcg
     2221 taacgccgag ctcccggaag acgaagaggt gcccggtccg cccgagcccg ctctggacct
     2281 catcgaggac gagcgacgcg ccgacgcggt cggcggcggc gcgcgcggcc gcgacgtact
     2341 cgggcgtgag cgggatgatg ccgccctctc ccaggatcgg ctcgaggaag atcgcggacg
     2401 tgtccggcgt gacggcggcg ttcagggccg cgacgtcgtt cggcggcagg aagaccgtcg
     2461 tcccgacgag cggctcgaac ggcttccggt aaccctcgtt gcccgtcagg gccagggcgc
     2521 ccatcgtgcg gccgtggaaa ccgccctcga gcgcgatcat ccggctcctg ccgggcgtcg
     2581 cgaggcgcgt gaacttcacg gcggcttcgg tggcctcggt ccccgtgttg cagaagaaga
     2641 ccttcgggaa gcccgagaga gcgacgagcc tctcggcgag gaggccctgc gccggctggt
     2701 agaagaggtt cgagacgtgg aggacgcggc ccgcctcgtc cttcagggcc ttcacgaggc
     2761 gcgggtgcct gtacccgagc gcgttcacgg cgatcccggc aaggaggtcc cagtagacct
     2821 tcccgtccgc gtcgacgagc ttcgcgccct tccccgagcg cgggtggaac gacgtgcgct
     2881 tgtaagtccc gaggacgtac gcggcgtccc gcgcctcgac ggcggcggtg tcttcagccg
     2941 atgtttgcaa tgcgagtccc tccgattccc tcggcgaggg ccgaggcgtg gcgcccaggc
     3001 ccggcgatga cgacctgcga gacgccggcc ttgagcgcct cgagacaggc gacgagcttc
     3061 ggacgcatgc cgcccgcgac gacgcccgag ttcaggagct cccacgtcgc gtcgggcccg
     3121 agcccctcga tgacttttcc gttcccgtcg aggaccccct cgacgtccgt cagaaagacg
     3181 agccgctcgg cctggagcgc cacggcgaga gcggacgccg ccgagtcggc gttcacgttc
     3241 aggagcgttc cttcacggcc gagcgcgacc gacgcgacga ccggcaggag ctcggccccg
     3301 aggatcgcgc ggatgagccg cggatccgag aacgtgacgc gcccgacgta accgaagtcg
     3361 acgccgtcca ggcgggggtg gaactcggcc cagagcgtct cgccgtccgc gcccgaaacg
     3421 cccgcggccg tgacgccgag ggagcgcagc tcggcgacga gcgacttgtt gacgaggccg
     3481 gacaggatgg acacgacgac gtccagcgtc tcggggtccg tgatccggag gccccggtac
     3541 gtcttcttcg ggatcttcag ggcctgcagc gacgcgtcga tgcgcttgcc gccgccgtgg
     3601 accacgacga cgttctcgcc ggacgtccag ctcgccgcga tggccgcgag ggcggcggcg
     3661 cgcggcacgg cgtcctcgag gaggcttccc cccagcttca cgacgatcgg gcggccgggg
     3721 cggtttgcgc tcaagcgagt ccctccgtct ccgggaatcc gaagacacgg ttcatgttct
     3781 gcacggcctg cgaggccgcg cccttgagaa ggttgtcgat caccgaaacg atcacgccgc
     3841 gccggccgcc ggacaggatc gcgaagccga tctccgcgcg cggcgttccg acgacgctgg
     3901 cgaggtcggg cagctcgccc gccggccgga cggacacgaa cggggcgtcc gcgtaccgcg
     3961 cgtacgcgcc ggcgacgtcg gcgggcccga caccggcgcg aaacgtgacg tgcagcgtcg
     4021 agaggatgcc cctcacgacc ggcaggaggt gcgggacgaa gacgaacggg gcgtccgcgg
     4081 aaaggccgag ctcctggcgc atctcgggct cgtgccggtg gcttccgacg ccgtacgcct
     4141 tgaagttccc ggccagctcg gagaacgaga acgcgaggtc ggacttcttc cccgcgcccg
     4201 acacgccgct cttgctgtcc gccacgacgg aggctccggg ctcgagcagc tcgagaacgg
     4261 gcttgagggc caggaggacg gacgtcggat agcacccggg gttcgcgacg agccgggcgc
     4321 cggagagctc cggcccgcac cactccgtca ggccgtagac ggcctcggcg aggagcgcag
     4381 gcgccgggtg gtcgaagccg taccacttcg ggtagtccga ggcgttccgc aggcggaacg
     4441 cccccgagac gtcgaccacc ctcgcgccga gcgcgaggat ctccgcggcc acctcggccg
     4501 acgtctcgtt cggcgtcgcg agaaagacga cgtcgggcct gcccgccgcg agggcctcga
     4561 gtgagaacac gtcggcggaa ggaccccgtg cgccgcccag cgacgggtgc aggcgcgaga
     4621 acgggaccgg ctttgcgtcc ttccggccgg acgagaacgc cgacgagacc gaggcgcgcg
     4681 ggtgccgcgc gaggatcgcg cagagttcgc cccccgagta gccggttgcg ccgacgacgg
//
LOCUS       DAOMFY010000299         4738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6452_length_4738_cov_6.103566, whole genome shotgun sequence.
ACCESSION   DAOMFY010000299 DAOMFY010000000
VERSION     DAOMFY010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6452_length_4738_cov_6.103566"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1011)
                     /gene="ispE"
                     /locus_tag="PLB01_17225"
     CDS             complement(91..1011)
                     /gene="ispE"
                     /locus_tag="PLB01_17225"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQQ79097.1"
                     /translation="MSEAREGLRVTSFAKINRDLRVLGKRKDGFHELDTVFQTVDLSE
                     EIDFLEGEEGGGQVSLTIEGAELPADDSNLILRAARALRERFSVRAGARIHLSKKIPI
                     GGGLGGGSSNAAATLRALTELWKITATEADLQAIAASLGSDVPFFLLGGRARGRGRGD
                     AVTPLPDGPQEWVLLVFPPFSLSTAEVYGRLKVRSLTGSPSATKVSGSETGGGPERND
                     LEQAAESLRGELRRIRSALAGAGARSARLSGSGSTVFGTFGTKEEASRAMTSLEGLGR
                     ETKARMAIVKTVSRAEFARRAAPVRIAQRG"
     gene            complement(1008..1562)
                     /locus_tag="PLB01_17230"
     CDS             complement(1008..1562)
                     /locus_tag="PLB01_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79098.1"
                     /translation="MPAPASASSPFEEISSSALAAYEAGRARAWDPSRRFKALFKAEV
                     SPKVGAIGRGWLSVWWDGASGSLEWRASAPIAGGGRGGLLRKGRGEGGVEPDSSPLPG
                     RLFSADLIACILGTPDAPPSPVTVLDERGNVVQMRFPNGEVVTFQPGSGVPRRIEANG
                     PDGRAVLTLESYGPWPSVEEVPPL"
     gene            complement(1688..3643)
                     /locus_tag="PLB01_17235"
     CDS             complement(1688..3643)
                     /locus_tag="PLB01_17235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ79099.1"
                     /translation="MPVRRVPAIVAFLLAAAAALPARAQTSGPALTPKEAKRKEAAEH
                     YLRARLFAQDTEFEQAVKEFRKAVDLDPEDGALRREYGELLRDIPIYPEAEREARKAV
                     ELSPRSAGAHRLLGQVLLATAKDKARLEEAAAELKKAAEIGPPDPQGAVALAQASIRL
                     DRPKDAVAALEAVAERAPGPAFYLLYGEALERDGQFEKAEDVYRSLLRMDPENRAASM
                     GLLRVYERSKQFDKEAALLESFMKGQPGNLAAKTQYATVLLRARRFPESRKAFEQVLA
                     ADPGNRDALRMYAALLSETRENEKADEILRRLQSLEPDDPEVAYRRAMNFLEARRLPE
                     AEQVLEDLRKALAAKKADGPEAGQIDGQLAYIAYLRKDYARATSLLTPRLKDGDGVNP
                     QAFNLLAQIARDQEDWAGGLRIAREAASGPGKKTPNVRATHAEFLIRSSEPENKAEGE
                     KILAALTAEDRPGVLAAADAWQRLERYGRAADAARVGLERAPDDPELLFRLAASLERD
                     KKIGESEAVFERLLKVRPDHAPGMNYLGYMWADRGENLQRALELIRKAVDLEPSNGAY
                     LDSLGWVYFRLDKLDKAEENLVAAATLNPDDATVEEHLGDLWNRKGDLRRGRESWKRA
                     LTLKPEPDVRQRLEEKLRKTESLDAKK"
     gene            complement(3688..>4738)
                     /locus_tag="PLB01_17240"
     CDS             complement(3688..>4738)
                     /locus_tag="PLB01_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQQ79100.1"
                     /translation="FPPRPSMRLVTDQFAFCARHVPRWNTVSISGYHIREAGSTALQE
                     LAFTLRDGMEYVKYGIDAGLDVDDFAPRLSFFFNAHNDFFEEIAKYRAARRIWAKVMR
                     DRYGAKKAESLKLRFHSQTAGVTLTAQQPYNNVVRVAVQSLAAVLGGTNSLHANALDE
                     TLALPTEFAAKIAVRTQQILLHETGVINTIDPLGGSYYVEELTNRMEAGAFDYFRRID
                     AFGGMVEAIEAGFPQKEIMDAAYRFQRAYDAKDKIMVGANAFTESLPEDEVSTLVISE
                     ETAAEQVASLNEVRRTRDSRTAMQALADLKAACAGGANVMPPLVEAVKAYATVGEISD
                     VMREVFATYEEPAVF"
BASE COUNT          683 a         1650 c         1625 g          780 t
ORIGIN      
        1 acctccggta ggcggttcca aagaccgctg acttaccact tgtcgactcc ccatccggaa
       61 gcctcacgtg gattgtcgcg caggcggcgt tcatccgcgt tgcgcgatcc ggaccggagc
      121 cgcgcggcgc gcgaactcgg cccgggaaac ggtcttcacg atcgccatcc tcgccttcgt
      181 ctctcttccg agcccctcga gcgacgtcat cgcccgcgag gcctcttctt tcgtcccgaa
      241 cgtcccgaaa accgtcgatc cgctcccgga aagccgggcg ctccgcgcac cggctcccgc
      301 gagcgcggac cggatgcggc gcaattcccc ccggagagat tccgccgcct gctcgagatc
      361 gttccgctcc ggcccgccac cggtctcgga gccggaaact ttagtggcag aaggacttcc
      421 cgtcaaggac cggaccttga gacgcccgta aacctctgcc gtggaaaggg aaaagggagg
      481 aaacacgagg aggacccact cctgggggcc gtcgggaagc ggtgtcacgg catcccccct
      541 gcccctgccc cgggcgcggc ccccgaggag gaagaacggg acgtcggacc cgagggaggc
      601 ggcgatcgcc tgcagatcgg cctcggttgc ggtgattttc cagagttcgg tcagcgccct
      661 caaggtcgcg gctgcgttgg aactgccgcc cccgagccct ccgccgatgg ggattttctt
      721 tgaaaggtga atgcgggcgc ctgcgcgcac cgagaacctc tcgcggaggg cacgggcggc
      781 gcgaagaatg agattgctgt cgtcggctgg cagctcggcc ccctcgatcg tgagcgagac
      841 ctgcccgccg ccttcttcac cttctaagaa atctatctcc tctgaaaggt ccacggtctg
      901 gaagacggtg tccagctcgt gaaagccatc cttgcgcttt cccagcaccc tgaggtcgcg
      961 gttgatcttc gcgaacgacg tgacgcggag gccttcgcgc gcctcgctca cagcggcggg
     1021 acctcttcga ccgagggcca cggcccgtag ctctcgagcg tcagcacggc gcggccgtcc
     1081 ggaccgttcg cctcgatgcg gcggggcacg cccgaaccgg gctgaaacgt cacgacctcg
     1141 ccgttcggaa aacgcatctg gacgacgtta cccctctcgt cgaggacggt aacgggcgat
     1201 ggaggagcgt ccggcgtgcc gaggatgcag gcgatgaggt cggcggaaaa gagcctgccg
     1261 ggaagaggtg acgagtccgg ctccacgccg ccctcccctc ttcccttcct gagcagtccc
     1321 cctcttcctc ccccggcgat cggcgcggac gcccgccatt cgagcgagcc gctcgcgccg
     1381 tcccaccaca cggagagcca gccgcggccg atcgcgccca ccttcggcga gacctcggcc
     1441 ttgaagagag ccttgaagcg gcgggaggga tcccacgccc tcgcgcgccc ggcctcgtag
     1501 gctgcgaggg ccgaagagga gatttcttcg aaaggtgagg acgcagaggc gggggcgggc
     1561 accccggcat cactcgacgc gcgcacgggc tgcccgctgc cgcatccacc ttctaagaag
     1621 aaaacaagcg aaacgagaac cgagcctgca acccgcagcg acggagggcg tcccgcggcg
     1681 cgcgtcctca cttcttcgcg tcgagacttt cggtcttccg gagcttctct tcgaggcgct
     1741 gtctgacgtc gggctcgggt ttcagcgtga gcgcgcgctt ccagctctcg cggcccctcc
     1801 ggaggtcgcc ctttctgttc cagaggtcgc cgaggtgctc ctcgacggtc gcgtcgtccg
     1861 ggttcagcgt cgcggcggcg acgaggttct cctcggcctt gtcgagcttg tcgaggcgga
     1921 aatagaccca gccgagcgaa tcgaggtacg ccccgttcga gggctcgaga tcgaccgcct
     1981 tgcggatgag ctccagcgcg cgctgaaggt tctcgccgcg gtcggcccac atgtacccga
     2041 ggtagttcat accgggcgcg tggtcgggcc ggaccttgag cagcctctcg aacaccgcct
     2101 cggactcgcc gatcttcttg tcccgctcca gcgacgcggc gaggcggaac aggagctcgg
     2161 gatcgtccgg cgctcgctcg agcccgaccc gggcggcgtc ggcggcgcgc ccgtaccgtt
     2221 cgaggcgctg ccaggcgtcg gcggcggcca ggacgccggg cctgtcctcg gcggtcagcg
     2281 cggcgaggat cttctcgccc tcggccttgt tttcgggctc ggacgagcgg atgaggaact
     2341 ccgcgtgcgt ggcgcgcacg ttgggcgtct tcttcccggg cccgcttgcc gcctcgcggg
     2401 cgatgcgcag gccgccggcc cagtcttcct ggtcgcgcgc gatctgcgcg agcaggttga
     2461 aggcctgcgg attgacgccg tcgccgtcct tcagccgcgg cgtcaggagc gacgtcgcgc
     2521 gcgcgtagtc ctttcggaga taggcgatgt acgcgagctg tccgtcgatc tggcccgctt
     2581 cgggcccgtc cgccttcttc gcggcgagcg ccttcctgag atcctcgagc acctgctcgg
     2641 cctcggggag gcgccgcgcc tcgaggaagt tcatcgcgcg gcggtaggcg acctcggggt
     2701 cgtccggctc gagcgactgg agcctccgca ggatctcgtc ggccttctcg ttctcgcgcg
     2761 tctcggagag gagggccgcg tacatcctca aggcgtcgcg gttgccggga tcggccgcga
     2821 ggacctgctc gaacgccttc cgcgactccg ggaagcgcct cgcgcgcagg agcaccgtgg
     2881 cgtactgggt cttcgcggcg aggttgccgg gctggccctt catgaacgac tcgagcagcg
     2941 cggcttcctt gtcgaactgc ttcgagcgct cgtacacgcg gaggagaccc atcgaagcgg
     3001 cgcggttctc ggggtccatc cggaggagcg agcggtagac gtcctcggcc ttctcgaact
     3061 ggccgtcgcg ctccagcgct tccccgtaga ggaggtagaa cgcggggccg ggcgcccgtt
     3121 ccgccacggc ctcgagcgcc gcgaccgcgt ccttcggcct gtcgagccgg atggaggcct
     3181 gcgcgagggc gacggcgccc tgcgggtccg gtggaccgat ctccgccgcc ttcttcagct
     3241 cggcggcggc ctcctcgagg cgcgctttgt ccttcgcggt cgcaaggagc acctgaccga
     3301 ggaggcggtg ggctccggcg ctccggggcg acagctcgac ggccttgcga gcctcgcgct
     3361 ccgcctcggg gtagatcgga atgtcgcgca ggagctcgcc gtactcgcgc cggagggctc
     3421 cgtcctcggg gtcgaggtcg accgccttgc gaaactcctt gacggcctgc tcgaactcgg
     3481 tgtcctgggc gaagagccgg gcccgcaggt aatgctcggc cgcttccttg cgcttcgcct
     3541 ctttcggcgt gagcgcgggg ccggaggtct gggcgcgcgc cggaagggcc gccgcggcgg
     3601 cgagcaggaa ggcgacgatc gcgggaacgc gtcgaacagg catcatcgag gagtctaacc
     3661 cgcaagaacc gggccgcatt cccgtcctca gaagacggcc ggctcttcgt aggtcgcgaa
     3721 cacctcgcgc atgacgtccg agatctcccc gacggtcgcg tacgccttca cggcctccac
     3781 gagcggcggc atgacgttgg cgccgcccgc gcaggccgcc ttcaggtcgg cgagcgcctg
     3841 catcgccgtc cgcgaatcgc gcgtccgccg gacctcgttc aggctcgcaa cctgttctgc
     3901 agccgtctcc tcggagatca cgagggtcga cacttcgtcc tcaggaaggg actcggtgaa
     3961 cgcgttcgcg ccgaccatga tcttgtcctt cgcgtcgtag gcgcgctgga accggtaggc
     4021 ggcgtccatg atctccttct gcgggaagcc ggcctcgatg gcctcgacca tgccgccgaa
     4081 cgcgtcgatg cgccggaagt agtcgaaggc gcccgcctcc atccggttcg tgagctcctc
     4141 gacgtagtac gagccgccga gcgggtcgat cgtgttgatg acgcccgtct cgtggaggag
     4201 gatctgctgc gtccgcaccg cgatcttcgc cgcgaactcg gtcggcaggg cgagcgtctc
     4261 gtcgagtgcg ttcgcgtgga gcgagttcgt cccgccgagc acggccgcca gggactggac
     4321 cgccacgcgc accacgttgt tgtacggctg ctgggccgtg agcgtgacgc cggccgtctg
     4381 cgagtggaag cggagcttca ggctctcggc cttcttcgcc ccgtagcggt cccgcatcac
     4441 cttggcccag atccgccggg ccgcgcggta cttggcgatc tcctcgaaga agtcgttgtg
     4501 cgcgttgaag aagaaggaga ggcgcggcgc gaagtcgtcg acgtcgagcc cggcgtcgat
     4561 cccgtacttc acgtactcca tcccgtcgcg cagggtgaac gccagctcct ggagcgcggt
     4621 cgagccggcc tcgcggatgt ggtagccgga gatcgacacc gtgttccagc gcggcacgtg
     4681 ccgcgcgcag aacgcgaact ggtccgtcac gagccgcatc gaggggcgcg gcgggaag
//
LOCUS       DAOMFY010000300         4682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6569_length_4682_cov_5.875081, whole genome shotgun sequence.
ACCESSION   DAOMFY010000300 DAOMFY010000000
VERSION     DAOMFY010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6569_length_4682_cov_5.875081"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..133)
                     /locus_tag="PLB01_17245"
                     /pseudo
     CDS             complement(1..133)
                     /locus_tag="PLB01_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            114..584
                     /locus_tag="PLB01_17250"
     CDS             114..584
                     /locus_tag="PLB01_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79101.1"
                     /translation="MRPRWPLPLLALSLAGALGSLALHAAWILGRPPAPLAVPIALEL
                     GIALVWLPAVAVFALRARARFGSPFSPALVRAFFPTFWDGTPPPLRRAAIASVAYAFL
                     FLFARWLWQLNDADVTWAAPGDPELTSLAFAFYVLSAAVLAGVGRERGPGPRAL"
     gene            609..1004
                     /locus_tag="PLB01_17255"
     CDS             609..1004
                     /locus_tag="PLB01_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79102.1"
                     /translation="MLVLAGAILLALGVASGAFLALAPFGFVAVAPGLTLWVLFPLFT
                     IAGFLLLAFPSRPPAVILLSRIAGGVLLALALLAALGLFAAASGTLKATSPTSALWYV
                     LGFGLLLGVAGLSGHRSAGHAPAAGATRP"
     gene            1012..1890
                     /locus_tag="PLB01_17260"
     CDS             1012..1890
                     /locus_tag="PLB01_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQQ79103.1"
                     /translation="MIIDLVPPRTADLDGGMSVARLLPRRQRRMVGPWCFLDAYGPLP
                     DGAPRMDVAPHPHVGLQTVSWLVSGSILHRDSLGSEQIVEPGTLGLMTAGRAIAHSEE
                     SPGGDASGLQGVQLWVALPDAHRRAEPSFEHHGRLPVEGFGAARATIFYGSLGKSASP
                     ARRFSPALGAEIIFPAAARATLPVDPGFEHAVLRLRGRIRADGVDLPKETLAYVAPGS
                     EALVLEGDADSLALVLGGAPFGEEILMWWNFVARTPDEMAKTRADWEAGVFGDVPGYE
                     GAPLPAPPLEPFSRRA"
     gene            complement(1962..2210)
                     /locus_tag="PLB01_17265"
     CDS             complement(1962..2210)
                     /locus_tag="PLB01_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010935994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HQQ79104.1"
                     /translation="MAMKIDETCINCGNCEPDCPNTAISQGDSYYVIAPEKCTECVGA
                     FDAPKCVEVCPVEGCITIDASLNESREVLQARYQQLHP"
     gene            complement(2411..2668)
                     /locus_tag="PLB01_17270"
     CDS             complement(2411..2668)
                     /locus_tag="PLB01_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79105.1"
                     /translation="MADPKDHSGEVHEAFDDLHEKLGNRATSDTKVRVEALREAVLAQ
                     DAVRVKAHLAEMKESHGWLWTELTAHPRVAELIDKLALMGL"
     gene            2713..3213
                     /locus_tag="PLB01_17275"
     CDS             2713..3213
                     /locus_tag="PLB01_17275"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQQ79106.1"
                     /translation="MSVFMDPLSKNLLFRLVFENRVASLGTLHDGAPFVSMVLTAPDP
                     THSVWFIHVSRLSGHTQDLLKDPRVSLMLAMPDTGEKDPQQLARLTLTGDATELENGS
                     DEEASAKEIYLGKFPHMADIVGQLGDFSFWVIRPKSARFVGGFARAFTLDPAALRAEI
                     AAGASA"
     gene            3210..3674
                     /locus_tag="PLB01_17280"
     CDS             3210..3674
                     /locus_tag="PLB01_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HQQ79107.1"
                     /translation="MTIPGLATRDVVPLLQVAVGPVILISGVGLLLLSMTNRFGRLID
                     RSRQLSRELRTASEDDRTGLIAQVRILTARAQIVRLAIALATTSLLLAALLIILLFLT
                     ALFGLELAVVLSLVFIACMAFLIASLVLFLRDINLSLHAFKLEIESAARHRP"
     gene            3725..4540
                     /locus_tag="PLB01_17285"
     CDS             3725..4540
                     /locus_tag="PLB01_17285"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79108.1"
                     /translation="MPALPLLPPEIASQLTTGADLADRERGNRRPATLPTRVPALDRL
                     LGGGLPRGSLVEMSGRASCGRFSAALSTLAAVTQTGEAAALVDPGDHFDPQGAADAGV
                     DLPRLLWLRPRALKTALSACETVLSTGFALVVLDLGLARVSRRRFDDAVWLRLARRAR
                     FHETALLVLSPYRVTGTAAHVVLSAGDARAAWDAERMAQPLLNGFTARIFLEKTRAHG
                     AEGRMREAHAPLRLQLLDAVNAGRTEENEEKKKIFLEREEGNAAFAEVAGLSR"
BASE COUNT          565 a         1762 c         1576 g          779 t
ORIGIN      
        1 cgtcgtgcgc aggatctgcc tcgatcgccg cggccagcct aactcgctgc ttgtcggtga
       61 ggttctccgc tccggcgcgc aggatggtct ggatcccgta gagcggctct cccttgcgtc
      121 cgcggtggcc gctcccgctc ctcgctctct cgctcgccgg cgcgctcggc agcctcgcgc
      181 tgcacgccgc gtggatcctg gggcggcctc cggccccgct cgccgtgccg atcgcgctgg
      241 agctcgggat cgctctcgtg tggctgccgg ccgtcgcggt cttcgccctg cgggcgcggg
      301 cgcgcttcgg gagcccgttc tccccggcgc tcgtgcgcgc gttcttcccg acgttctggg
      361 acggcacgcc gccgccgctg cggcgcgccg cgatcgcctc cgtcgcgtac gcctttctct
      421 tcctgttcgc gcgctggctc tggcagttga acgacgccga cgtcacgtgg gccgcaccgg
      481 gcgaccccga gctcacgtcc ctcgccttcg cgttctacgt cctctcggcg gccgtcctcg
      541 cgggcgtagg ccgggagcgc ggccccggtc cccgcgcgct ctgagagaat ctcgccggag
      601 gccttcccat gctcgtcctc gcaggcgcaa ttctcctcgc tctcggcgtc gcgtcggggg
      661 cgttcctcgc cctcgcgccg ttcggcttcg tggccgtcgc gcccggcctc acgctctggg
      721 tcctgttccc gctcttcacg atcgcgggct tcctcctgct cgcgttcccg tcgcgcccgc
      781 cggccgtcat cctcctgtca cggatcgccg gcggcgtcct cctcgcgctc gcgctcctcg
      841 cggcgctcgg cctgttcgcc gcggcgtcgg ggacgctcaa ggcgacgagc ccgacgtccg
      901 cgctctggta cgtgctcggc ttcggcctcc tcctcggcgt cgcaggcctc tcgggccacc
      961 gctccgcggg ccacgcgccc gcggcgggcg cgacgcggcc gtagtccgga cgtgatcatc
     1021 gacctcgtcc cgccgcgcac ggcggatctc gacggcggaa tgtccgtcgc gcggctcctc
     1081 ccgcgccggc agcgccggat ggtcgggccg tggtgcttcc tcgacgcgta cggcccgctc
     1141 cccgacggcg cgccgcgcat ggacgtcgcg ccgcacccgc acgtcggcct ccagaccgtc
     1201 tcgtggctcg tctcgggctc catcctccac cgcgacagcc tcggctccga gcagatcgtc
     1261 gagcccggca cgctcggcct catgaccgcg ggacgcgcga tcgcgcattc ggaggagtcg
     1321 ccgggcggcg acgcatcggg cctgcagggc gtgcagctct gggtcgcgct gccggacgcc
     1381 caccgcaggg ccgagccctc cttcgaacac cacggcaggc tcccggtcga gggcttcggg
     1441 gccgcgcgcg cgacgatctt ctacggctcg ctcggcaaga gcgcctctcc cgcgcgacgc
     1501 ttctcgcccg cgctcggcgc cgagatcatt tttccggcgg cggcccgcgc gacgctcccc
     1561 gtggaccccg gcttcgagca cgccgtgctg cggctccgcg ggaggatccg cgccgacggc
     1621 gtggacctgc cgaaggaaac gctcgcgtat gtggcgcccg ggagcgaggc gctcgtcctc
     1681 gagggcgacg cggacagcct cgccctcgtc ctcggcggtg cgccgttcgg cgaggagatc
     1741 ctgatgtggt ggaacttcgt cgcgcggacg ccggacgaga tggcgaagac gcgcgccgac
     1801 tgggaagccg gcgtcttcgg cgacgtgccg ggctacgagg gcgcgccgtt gccggcgccg
     1861 ccgctcgagc cgttctcacg ccgcgcctga aaaaaagaac acgggcgggg cgcttcgccg
     1921 cgccgccgcc cgcgctccct cgcggggaat ttaccggggg ctcaggggtg cagctgctgg
     1981 tagcgcgcct ggaggacttc cctcgactcg ttcagggacg cgtcgatcgt gatgcagccc
     2041 tcgacggggc agacctcgac gcacttcggg gcgtcgaacg cgccgacgca ctcggtgcac
     2101 ttctcgggcg cgatcacgta gtacgaatcg ccctggctga tcgccgtgtt cgggcaatcg
     2161 ggctcgcagt tgccgcagtt gatgcaggtc tcgtcgatct tcatcgccat ggtggtcacc
     2221 tctctccgac gcgaatggtc tcaggcgcaa ccggggcggc aaatgatgca gacaaatcac
     2281 cccgtgacgc gccgcgcaac cctctgcagg gacgtcgcgt agagtctccc catgggaccg
     2341 ctcgcgcgac cgccgcgtgc ctcctcgtct tccggtcgga gcgggtgaag aagttcttcc
     2401 ggctggaagc ctagaggccc atcagcgcga gcttgtcgat gagctcggcg acgcgggggt
     2461 gggccgtcag ctcggtccag agccagccgt gcgactcctt catctcggca aggtgcgcct
     2521 tcacgcggac cgcgtcctgc gcgagcacgg cctcgcgcaa ggcctcgacg cggaccttcg
     2581 tgtcggacgt cgcgcggttc ccgagcttct cgtggaggtc gtcaaaagcc tcgtggactt
     2641 cgccggagtg gtccttggga tcggccatgg gggtgattgt ctcaccctcc cgctcgaccc
     2701 gcgctcgcgc taatgtctgt gttcatggat cctctttcta agaatctcct cttccgtctc
     2761 gtcttcgaaa accgcgtcgc ctccctcggc acgctgcacg acggcgcgcc gttcgtctcg
     2821 atggtcctga cggcgcccga cccgacgcac tccgtgtggt tcatccacgt gagccggctc
     2881 tcggggcaca cgcaggacct cctcaaggac ccgcgcgtga gcctgatgct cgcgatgccc
     2941 gacacgggcg agaaggaccc gcagcagctc gcgcgcctga cgctcacggg cgacgccacg
     3001 gagctggaga acggctcgga cgaggaggcc tccgccaagg agatctacct cgggaagttc
     3061 ccgcacatgg ccgacatcgt cgggcagctc ggcgacttct ccttctgggt gatccggccg
     3121 aagtcggcgc ggttcgtggg cgggttcgcg cgggccttca cgctcgaccc cgccgcgctc
     3181 cgcgcggaga tcgcggccgg ggcgagcgcg tgacgattcc cggcctcgcg acgcgcgacg
     3241 tcgtcccgct cctgcaggtc gcggtcgggc cggtgatcct gatctcgggc gtcgggctgc
     3301 tgctcctctc gatgacgaac cgcttcggga ggctcatcga ccgctcgcga cagctctcgc
     3361 gcgagctgcg cacggcgagc gaggacgacc gcaccgggct gatcgcgcag gtgcgcatcc
     3421 tcacggcgcg ggcgcagatc gtccgcctcg cgatcgcgct cgccacgacg agcctcctcc
     3481 tcgcggcgct cctcatcatc ctgctgttcc tgacggcgct cttcgggctg gagctcgccg
     3541 tcgtcctctc gctcgtcttc atcgcgtgca tggcgttcct catcgcgtcc ctcgtcctct
     3601 tcctgcgcga catcaacctc tccctgcacg ccttcaagct ggagatcgag tccgccgccc
     3661 ggcaccgccc ctgacccgat aacttacgct tgacttacgt cttctccgga cctagcttcg
     3721 gggagtgcct gcgctgcccc ttcttccgcc cgaaatcgcc tcccagctga cgacgggggc
     3781 cgacctcgcc gaccgcgagc gcggcaaccg gcggcccgcg acgctgccca cgcgggtgcc
     3841 ggcgctcgac cgcctgctgg gcggagggct gccgcggggc agcctggtag aaatgtcggg
     3901 ccgcgcctcc tgcgggcgct tctcggcggc gctttccacc ctcgcggccg tgacgcagac
     3961 gggcgaggcc gccgcgctcg tcgaccccgg cgaccacttc gacccccaag gcgccgccga
     4021 cgcgggcgtg gacctccccc gcctcctctg gctgcgcccg cgcgcgctga agacggccct
     4081 ctccgcgtgc gagacggtgc tctccacggg cttcgcgctc gtcgtcctcg acctcgggct
     4141 cgcgcgcgtc tcgcgccggc gcttcgacga cgccgtgtgg ctgcgcctcg cgcgccgcgc
     4201 gcgcttccac gagacggcgc tcctcgtcct ctcgccgtac cgcgtcacgg gcacggcggc
     4261 gcacgtcgtg ctgtcggcgg gcgacgcgcg cgccgcgtgg gacgcggagc gcatggccca
     4321 gccgctgctg aacggcttta cggcgcgcat cttcctcgag aagacgcgcg cccacggggc
     4381 ggaggggcgc atgcgcgaag cgcacgcgcc cctccgcctc cagctgctgg acgcggtcaa
     4441 tgcggggagg accgaagaga acgaagagaa gaagaagatt ttcttagaaa gagaagaggg
     4501 gaacgcggca tttgccgagg ttgccgggct cagccgctga gttggagggt gccggggata
     4561 ttcactttca aagaaatccg aaagcggcga aacacgaagc attgcgcagc agcgaagcgg
     4621 ggcggttcgg gctgcacggg gtattcacct tcaaagaaat ccgaatcccg aaatccgaaa
     4681 tc
//
LOCUS       DAOMFY010000301         4664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6601_length_4664_cov_9.768496, whole genome shotgun sequence.
ACCESSION   DAOMFY010000301 DAOMFY010000000
VERSION     DAOMFY010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_4664_cov_9.768496"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3254
                     /locus_tag="PLB01_17290"
     CDS             <1..3254
                     /locus_tag="PLB01_17290"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQQ79109.1"
                     /translation="LLIDDAIVVRENIVRHLEKGQDHMSAARDGTAEIGLAVLATSLS
                     IIAVFVPVAFMKGIMGRFFFQFGMTVAFAVLVSLFVSFTLDPMLSSRWVDPDVERKGK
                     RSAVARVLDRFNGWFDRTADRYRALVGWALDHRRIVLGLAAAAFFGGLLLFGTLQSEF
                     MVNADQAEFQIRFKTAPDASFEETRGRMGAVLAELKKMPEVKHTYASIGAGETGTVRD
                     ARVYVKLVEAKERKKHATVLAAEARRRIAPIAGIIPSVEIQTDEFAEKPIMVSIRGEE
                     IPLLKKYAAELKDAVYGIPGIVDLEMTLELDLPEYRLVVDRERASDAGLSTPAIARTI
                     GALVGGQAVTTYEDEEGEAVDVRVRLPLQMRRDVSQIGDLRIAVPGGKKGHALVPLSE
                     LVRAERATTPSEISRRDLSREVLLTANLDGVPLGTAVQKIREASAKIPMAPGYKIYFP
                     GEAERMEESFGYMAEALLLAIVFVYLILAAQFESFIEPLSIMLSLPLSIVGMAGMLAA
                     TGDTVNIMSLIGLILLMGLVTKNAILLVDYTKVLRARGMDRRTALVTAGRTRLRPIMM
                     TTLAMIFGMLPLALGLGQGAELRSPMGRAVIGGLITSTVLTLLVVPVVYTLFDDFATW
                     LHRRWAKANAQEADAHVAPAAVKTVVSALLTAALLVPAGSLAAGAGETTAPAAARVLT
                     LADAVAIAMDKNRDVEKARSYQDWVRGKYVEERAAALPIVSAEAGWARSWDGTFAALT
                     GGLFPSGQTTQAAGLTLSQTLFAWGKVGAAVRAAKQGIAAADDQLEHFRQAAVRDVTE
                     SFYDVLLARRLAEISKETLRLREQQLTQAEQRHALGTATDYDVLAARVALDNQKPEVL
                     RTANLVLIALDRLRLVLAEETAALDVTGTLESGPEEIPSFDTAVRTALERRPDVRGLE
                     HGVGVYREFVKIRNADDKPRLDLRANAGWTWLEADPIAVDGKKWGIGVAFSFPFFDGL
                     ATRGRVLQAKSDLTRAELDLSAARDRINVEVRTVLDQAKVAGEIVAALSGNVDQARRF
                     LEMSEKGQEFGVKTRLEVDDALVNVRQAEGNLARAKRDYLVALTNLRYVQGTL"
     gene            complement(3262..3349)
                     /locus_tag="PLB01_17295"
     tRNA            complement(3262..3349)
                     /locus_tag="PLB01_17295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3313..3315),aa:Ser,seq:tga)
     gene            3412..3505
                     /locus_tag="PLB01_17300"
     tRNA            3412..3505
                     /locus_tag="PLB01_17300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3446..3448,aa:Ser,seq:gct)
     gene            3594..>4664
                     /locus_tag="PLB01_17305"
     CDS             3594..>4664
                     /locus_tag="PLB01_17305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ79110.1"
                     /translation="MSTRIKAAIGIAVVAVLAVVVWASVARKDKNTVRVTTAKVGKVP
                     LVSTVSCNGRVRAKTKVDISSQVMGQIVTLAVREGDHVKKGDLLLQIDKAQYDAGAQA
                     TQAGLDALFAQRESDRATREQAQRDYAREQKNFDAKITSEQALQKAKLSLDAAAANER
                     ATERRIEQARANLMGNKDSLRKTTLTSPIDGVVTARPVEQGENAIIGTMNNPGTVLLT
                     VSDMSIVEGEMEVDETDIPHVKLGQKATLTFDAYPDKKYEGKVTEIGGSPITKSALGT
                     DSSAVNFKVKVQVTNPPGNIRPGFSVSGKIETDRREAALAIPIPALVVADPASLERPK
                     PGKKDDKAKPAAGAAVAAPAPAT"
BASE COUNT          777 a         1624 c         1601 g          662 t
ORIGIN      
        1 gcctcctgat cgacgacgcg atcgtcgtcc gcgagaacat cgtccggcac ctcgagaagg
       61 ggcaggacca catgtcggcc gcccgcgacg gcacggcgga gatcggcctc gcggtcctcg
      121 cgacgagcct gtcgatcatc gcggtgttcg tccccgtcgc cttcatgaag ggcatcatgg
      181 gccgcttctt cttccagttc gggatgaccg tcgccttcgc ggtgctcgtc tccctcttcg
      241 tctccttcac gctcgacccg atgctctcct cgcgctgggt ggaccccgac gtcgagcgga
      301 aagggaagcg cagcgcggtc gcgcgcgtcc tcgaccgctt caacggctgg ttcgaccgca
      361 cggccgaccg gtaccgggcc ctcgtcggct gggccctcga ccaccgccgg atcgtcctcg
      421 gcctcgcggc ggccgcgttc ttcggcgggc tgctcctgtt cgggacgctc cagagcgagt
      481 tcatggtcaa cgcggaccag gcagagttcc agatccggtt caagacggcc ccggacgcgt
      541 cgttcgagga gacgcgcggc cggatgggcg ccgtgctcgc cgagctgaag aagatgcccg
      601 aggtgaagca cacatacgcc tcgatcggcg cgggcgagac cggcaccgtc cgcgacgcgc
      661 gtgtctacgt caagctcgtc gaggcgaagg agcggaagaa gcacgcgacg gtcctcgccg
      721 ccgaggcccg ccggcggatc gccccgatcg ccgggatcat tccgtcggtc gagatccaga
      781 ccgacgagtt cgccgagaag ccgatcatgg tgtcgatccg cggcgaggag atcccgctcc
      841 tcaagaaata tgccgcggag ctcaaggacg ccgtgtacgg gatccccggc atcgtcgacc
      901 tcgagatgac gctcgagctc gacctgcccg agtaccggct cgtcgtggac cgagagcgcg
      961 cctcggatgc cggcctctcc acgcccgcga tcgcccggac gatcggcgcc ctcgtcggcg
     1021 ggcaggccgt gacgacgtac gaggacgagg agggcgaggc ggtcgacgtg agggtccgcc
     1081 tcccgctcca gatgcgccgc gacgtctcgc agatcggcga cctgaggatc gcggtcccgg
     1141 gcgggaagaa ggggcacgcg ctcgtccccc tctcggagct cgtccgggcc gagcgggcga
     1201 cgacgccctc ggagatctcg cgccgcgacc tctcgcgcga ggtcctcctc acggcgaacc
     1261 tcgacggggt cccgctcgga acggcggtcc agaagatccg ggaggcttcc gcgaagatcc
     1321 cgatggctcc cggctacaag atctactttc cgggcgaggc cgagcggatg gaggagagct
     1381 tcgggtacat ggccgaggcg cttctcctcg cgatcgtctt cgtctacctc atcctcgcgg
     1441 cccagttcga gtccttcatc gagccgcttt ccatcatgct gtcgctcccg ctttcgatcg
     1501 tcgggatggc cgggatgctc gcggcgacgg gcgacaccgt gaacatcatg tcgctcatcg
     1561 ggctcatcct cctgatgggc ctcgtcacga agaacgcgat cctcctcgtc gactacacga
     1621 aggtgctccg cgcgcggggg atggaccgcc ggaccgcgct cgtcaccgcg gggaggacgc
     1681 ggcttcgccc gatcatgatg acgacgctcg ccatgatctt cggaatgctc ccgctcgcgc
     1741 tcggcctcgg gcagggggcc gagctgcggt ccccgatggg gcgggccgtc atcggcggcc
     1801 tgatcacgtc gacggtcctc acgctcctcg tcgtccccgt cgtgtacacg ctcttcgacg
     1861 acttcgccac gtggctccac cggcgctggg cgaaggcgaa cgcccaagag gcggatgcgc
     1921 acgttgcgcc ggcggccgtg aagacggtgg tgtcggcgct gctcaccgcc gcgctgctcg
     1981 tcccggccgg gagcctcgcc gcgggcgcgg gggagacgac cgcgccggcg gcggcccggg
     2041 tcctgacgct cgcggacgcc gtggcgatcg ccatggacaa gaaccgcgac gtcgaaaagg
     2101 cgcgcagcta ccaggactgg gtccgcggca agtacgtcga ggaacgcgcc gcggccctcc
     2161 cgatcgtctc ggccgaggcc ggctgggcgc gctcgtggga cgggacgttc gcggcgctca
     2221 cgggcggcct gtttccgtcg ggccagacga cgcaggccgc agggctcacg ctgtcccaga
     2281 cgctcttcgc gtggggaaag gtgggtgcgg ccgtccgcgc cgccaagcag gggatcgcgg
     2341 cggcggacga tcagctcgag cacttccgtc aggccgccgt gcgcgacgtc accgagtcct
     2401 tctatgacgt cctgctcgcg cggcggctcg cggaaatctc gaaggagaca ctgcggttga
     2461 gagagcagca gctcacgcag gccgagcagc gacacgcgct cggcacggcg acggactacg
     2521 acgtcctcgc ggcgcgcgtc gcgctcgaca accagaaacc cgaggtgcta cgcaccgcga
     2581 acctcgtcct gatcgccctc gaccgcctga ggctcgtcct cgcggaggag accgccgcgc
     2641 tcgacgtgac cgggacgctc gagtccggac cggaggagat cccgtcgttc gacaccgcgg
     2701 tccggacggc cctcgagcgc cggccggacg tgagggggct cgagcacggc gtcggggtct
     2761 accgggaatt cgtgaagatc cggaacgcgg acgacaagcc ccggctggac ctgcgtgcga
     2821 acgccggttg gacgtggctc gaggcggatc cgatcgcggt cgacggaaag aaatggggaa
     2881 tcggcgtcgc gttctcgttc ccgttcttcg acggcctcgc cacgcgcggc agggtcctcc
     2941 aggcgaagag cgacctcacg cgcgcggagc tcgacctctc cgcggcgcgc gacaggatca
     3001 acgtcgaggt ccggacggtc ctcgaccagg cgaaggtcgc cggcgagatc gtcgccgccc
     3061 tctcgggaaa cgtggaccag gcccggcgct ttctcgagat gtccgagaag gggcaggagt
     3121 tcggcgtgaa gacgcgcctc gaggtcgacg acgccctcgt gaacgtgcgc caggccgagg
     3181 gaaacctcgc ccgggcgaag cgtgactacc tcgtcgcgct cacgaacctc aggtacgtgc
     3241 agggaacgct ctgagggagg ctggcggagg aggagggatt cgaacccccg gtacctttcg
     3301 gtacaacggt tttcaagacc gccgccttaa accactcggc cactcctccg gaaacgcgcg
     3361 gaaagccatt atctcctctc cctgctagac ttcgcggccc ggcgccgcca cggacaggtg
     3421 ggtgagtggc ttaaaccagc ggtttgctaa accgccgtag ggctttaaaa ccttaccggg
     3481 ggttcgaatc cccccctgtc cgccagccct tcgtccccgg ccgtgaaact tcgcggccgg
     3541 aaacctcgta acagcggctg gagtcagcat ttgcaccgga agggagccgt tccatgagca
     3601 cgcggatcaa ggcggcgatc ggaatcgcgg tcgtcgcggt cctggccgtc gtcgtctggg
     3661 cgtcggtcgc ccgcaaggac aagaacaccg tgcgggtcac gaccgcgaag gtcgggaagg
     3721 tgccgctcgt ctcgaccgtg agctgcaacg gccgcgtccg cgccaagacg aaggtcgaca
     3781 tctcgtcgca ggtgatggga cagatcgtga cgctggcggt gcgggaaggc gaccacgtca
     3841 agaagggcga cctcctcctc cagatcgaca aggcccagta cgacgccggc gcccaggcga
     3901 cgcaggccgg cctcgacgcc ctcttcgcac agcgcgagtc cgaccgcgcg acgcgcgagc
     3961 aggcccagcg cgactacgcg cgcgagcaga aaaacttcga tgcgaagatc acgtccgagc
     4021 aggcgctcca gaaggcgaag ctctcgctcg acgcggccgc cgcgaacgag cgggccaccg
     4081 agcggcgcat cgagcaggcg cgcgcgaacc tcatgggaaa caaggacagc ctccggaaga
     4141 cgacgctcac gtcgccgatc gacggcgtcg tgacggcgcg cccggtcgag cagggcgaga
     4201 acgcgatcat cgggacgatg aacaaccccg gcaccgtcct cctgaccgtt tcggacatgt
     4261 cgatcgtcga gggcgagatg gaggtcgacg agaccgacat cccgcacgtg aagctcggcc
     4321 agaaggcgac gctcaccttc gacgcgtacc cggacaagaa atacgaaggc aaggtcacgg
     4381 agatcggcgg cagcccgatc acgaagtcgg ccctcggcac ggactcgtcc gccgtgaact
     4441 tcaaggtcaa ggtccaggtc acgaacccgc ccggaaacat ccggcccggt ttctccgtgt
     4501 ccggcaagat cgagacggac cgccgcgagg ccgccctcgc gatcccgatc ccggcgctcg
     4561 tcgtcgcgga tccggcctcg ctcgagcgcc caaagcccgg caagaaggac gacaaggcga
     4621 agccggccgc cggcgcggcc gtcgcggcgc ccgcgccggc gacc
//
LOCUS       DAOMFY010000302         4662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6603_length_4662_cov_7.382027, whole genome shotgun sequence.
ACCESSION   DAOMFY010000302 DAOMFY010000000
VERSION     DAOMFY010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6603_length_4662_cov_7.382027"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLB01_17310"
     CDS             <1..642
                     /locus_tag="PLB01_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79111.1"
                     /translation="WVVRTPHHPAPPAVDARLEAPWWRAAGAEDASVYRFEAGRAVEP
                     GEFVWSFSGGAVRFPKGGGVSSRDAIETYSKDVTVLAAEGPWADGSVTATVRLMDDLG
                     SAHNWVGVAARRAKPGDGFRDSGVLGLLRGNGEAVLFGPGGERKAATGVDPRRGPVRL
                     ALVCRGPRVAFLVEGREVAAVEDAPVAPGWAGVHAWGLARVTELSARGAPSFP"
     gene            complement(631..987)
                     /locus_tag="PLB01_17315"
     CDS             complement(631..987)
                     /locus_tag="PLB01_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQQ79112.1"
                     /translation="MRYVLGFFPPDRLFAIVDALSESHAHGLTISDARGFGQEHDVSH
                     PEHREHLGVEMTKKIRVEIVCRDDEADVLVDALYRAGHTGRRGDGKVFVLPVLEALRV
                     KTGERGEAALGPSSGE"
     gene            complement(987..4088)
                     /locus_tag="PLB01_17320"
     CDS             complement(987..4088)
                     /locus_tag="PLB01_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQQ79113.1"
                     /translation="MIGALVAWSLRRRVIVLALLVVFVAAGVLAFKRLPVEAYPDVTN
                     VQVQIITLFAGHAAEEVERLVTIPVENEMNGIPKRASMRSISIFGLSVVTIVFEDDAD
                     RAYVRNQAFERLQSVALPQGAQANLSPDSTPVGEIYRYTVTGPPGISPLELKSLEDWV
                     VERRLRTVPGVVDVVGFGGPTKQYQVNVDPLKLKSYGLTLPQVFDALAKGNRNAGGAY
                     IEHGAEMYVVRGLGLVQSPADIEEIAVVTRNGTPIRIRDVGSVGVGPRIRLGRVGKTV
                     GSSDEDDVVQGIVLLRKGENALEVLERVRAKVAEINRGGLPPGVAIVPHYDRTALIDR
                     TLHTVRRNMAEGIGFVLVVLVAFLGVANARSALVVAAVVPLSLLGAFLLLDLRGIPAN
                     LISMGAIDFGIIVDSAVVVVENLLRILEERGSKVRSLSAAIVQATSEVGKPILFSKAI
                     LITAFLPLYTLQRVEGKIFRPMALTLTFALLWGTALALTVVPVLASFAVKLRGAERAH
                     EEAESASVRFLTHLYRPALGWVLSRRRVVVAASAALLAAGVATALLIGSEFLPKLDEG
                     SLWVRVTMPGSISPTEAGKTVARVRAILAAFPEVRTVVSQLGRPDDGTDVNGFDTAEL
                     FVDLFPRDAWKTAKTRDALADAMSRRLAAIPGVEFNFSQVIEDNVNEAISGIKGELGI
                     KIFGEDPAVLQDLADRAAAALDDVPGAADVAAERLAGQPQVQISVDRPAIGRYGLSVS
                     DVQTVIETALGGAVATQILEGEKTFDLVVRVDPGAVTGIDSIRAIPILGPSGEKLTLG
                     SVADVRLAPGSARVFREENSRRIAVKLSVRGRDLGSLVSEAQRKVDAAVKLPPGYRLE
                     WTGAFENQQRALRRLAVVLPITLAAIFFLLFAAFDSPRLATLILLNVPFAAVGGLFAL
                     PLAGLTLSVSALVGFIALFGVSVQNGVLLVERIRELRHAGKALAEAVTEGALTRFRPV
                     LMTAAMAAFGLLPAALSHAVGAETQRPFAVVIIGGLVTATLLTLFVLPVLYELFEREE
                     EEY"
     gene            complement(4085..>4662)
                     /locus_tag="PLB01_17325"
     CDS             complement(4085..>4662)
                     /locus_tag="PLB01_17325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQQ79114.1"
                     /translation="RLGSPLPGVVVERNANPGQEARPDAAAPLFVVTNPKSLWVWLDV
                     TERDLALLAPGDRLSIRSAAWPGRVFTGRLDLVGDALDPATRTVRARGAVDNPEGHLK
                     AEMYVTVEVSGRSAQPATVVPARAILTDAAGRVCFVEAGPSRFRRTRVTVGPERGGKV
                     PVLAGLPEGARVVTDGSLLLSSLFASAGEKK"
BASE COUNT          728 a         1589 c         1718 g          627 t
ORIGIN      
        1 tgggtcgtgc gcacgccgca ccatcccgcg cccccggcgg tcgacgctcg cctcgaggcg
       61 ccgtggtggc gggcggccgg ggcggaagac gcgtcggtct accggttcga ggccggccgg
      121 gccgtcgagc ccggggagtt cgtctggagc ttctccggcg gcgccgtgcg ctttccgaag
      181 ggcggaggcg tctcctcccg cgacgcgatc gagacgtatt cgaaggacgt caccgttctc
      241 gcggccgaag gcccctgggc cgacggctcc gtgaccgcca cggtgcgtct catggacgac
      301 ctcggctccg cgcacaactg ggtcggcgtc gccgcgcggc gggcgaagcc aggcgacggc
      361 ttccgcgact cgggagtcct cggactcctg cgtggaaacg gcgaggccgt tctgttcgga
      421 ccgggcggcg agaggaaagc cgcgaccggc gtcgacccgc gccgcgggcc ggtgcgcctc
      481 gcgctcgtct gccgcggccc ccgcgtcgcg ttcctcgtcg agggacggga ggtcgccgcg
      541 gtcgaagacg ctcccgtcgc tcccggatgg gcgggcgtgc acgcctgggg cctcgcgcgt
      601 gtgacggagc tcagcgcgcg gggcgccccc tcattcccct gaggacgggc cgagcgcggc
      661 ctcgccgcgc tcgccggtct tgacccgcag cgcctcgagg acgggcagga cgaagacctt
      721 gccgtcgcct cgacggcccg tatggccggc ccggtaaagc gcgtcgacga gcacgtcggc
      781 ttcgtcatcg cggcagacga tctccacgcg gatcttcttc gtcatctcga ccccgaggtg
      841 ctcgcggtgc tccgggtggg agacgtcgtg ctcctgcccg aatccgcgcg cgtcggagat
      901 cgtcagtcca tgcgcgtgcg actccgagag ggcgtccacg atcgcgaaga gacggtccgg
      961 cggaaaaaag ccgaggacgt aacgcatcag tattcctcct cctcgcgttc gaacagctcg
     1021 tagaggacgg gcaggacgaa gagcgtgagc agcgtggcgg tgacgagacc gccgatgatg
     1081 acgacggcga acggacgctg ggtctcggcg ccgacggcgt gcgagagcgc ggccggaagc
     1141 aggccgaagg cggccatcgc cgccgtcatg agaacgggcc ggaaccgggt cagggcccct
     1201 tcggtgacgg cctcggccag cgccttgccc gcgtggcgca gctcgcggat gcgctcgacg
     1261 aggaggacgc cgttctggac ggagactccg aacagcgcga tgaatccgac gagagccgac
     1321 acggagagcg tgaggcccgc gagcgggagc gcgaagagcc ctccgaccgc cgcgaacggg
     1381 acgttgagga ggatgagcgt cgcaaggcgc ggcgagtcga acgccgcgaa gagcaggaag
     1441 aagatggccg cgagcgtgat cgggaggacg accgcgagcc gcctgagggc tctctgctgg
     1501 ttctcgaacg cgccggtcca ctcgaggcgg tagccgggtg gaagcttgac ggcggcgtcg
     1561 accttccttt gcgcctccga gacgagggac ccgagatcgc gcccgcgcac ggagagcttc
     1621 acggcgatgc ggcgcgagtt ctcctcgcgg aagacgcggg cggagcccgg cgcgaggcgc
     1681 acgtccgcga cggagccgag cgtgagcttt tcgccgctcg gcccgaggat cgggatcgcc
     1741 ctgatcgagt cgatcccggt cacggcgccc gggtcgacgc gaacgacgag gtcgaacgtc
     1801 ttctcgccct cgaggatctg cgtggccacg gcgcccccga gcgccgtctc gatgaccgtc
     1861 tggacgtccg aaacggagag gccgtagcgc ccgatcgccg gccggtcgac cgagatctgg
     1921 acctgcggct ggccggcaag gcgctcggcg gcgacgtcgg ctgcgcccgg cacgtcgtcg
     1981 agggcggcgg cggcccggtc cgcgaggtcc tggaggacgg cgggatcctc tccgaagatc
     2041 ttgatcccga gttcgccctt gatgcccgag atcgcctcgt tcacgttgtc ctcgatgacc
     2101 tgcgagaagt tgaactcgac gccggggatc gcggccagcc ggcggctcat ggcgtcggcg
     2161 agcgcgtcgc gggtcttcgc ggtcttccac gcgtcgcgag ggaagaggtc gacgaagagc
     2221 tccgcggtgt cgaaaccgtt cacgtcggtc ccgtcgtccg gccgcccgag ctggctcacg
     2281 acggtcctca cctcgggaaa cgctgcgagg atggcacgca cgcgcgcgac cgtcttgccg
     2341 gcctccgtgg gcgagatcga tcccggcatc gtcacgcgga cccagagaga gccctcgtcg
     2401 agcttcggaa ggaactcgga accgatgagg agcgccgtcg cgacccccgc ggcgagaagc
     2461 gcggccgagg cggcgacgac gaccctccgg cggctgagca cccagccgag cgcgggccgg
     2521 tagagatgcg tcaggaaccg gaccgacgcg gattcggcct cctcgtgcgc gcgttcggcg
     2581 ccacggagct tcacggcgaa gctcgcgagg accgggacga ccgtgagcgc gagggccgtc
     2641 ccccagagga gcgcgaacgt cagcgtcagc gccatcgggc ggaagatctt tccctccacg
     2701 cgctgcagcg tgtagagcgg caggaacgcc gtgatgagaa tcgccttcga gaagaggatc
     2761 ggcttgccga cctcgctcgt ggcctggacg attgcggccg aaagcgagcg gaccttgctg
     2821 cctcgctcct cgaggatcct caggaggttc tcgacgacga cgacggccga atccacgatg
     2881 atcccgaagt cgatcgcccc catcgagatg aggttcgcgg ggatcccgcg cagatcgagc
     2941 agcaggaacg ccccgagcag cgagaggggg acgaccgccg cgacgacgag ggcggaccgc
     3001 gcattcgcga cgccgaggaa cgcgacgagc acgacgagga cgaacccgat cccctcggcc
     3061 atgttccggc gcacggtgtg gagcgtgcgg tcgatgagcg ccgtgcggtc gtaatgcggg
     3121 acgatcgcca cgccgggcgg cagcccgccc cggttgatct cggccacttt cgcccggacc
     3181 cgctcgagaa cctcgagggc gttctcaccc ttgcggagga ggacgatgcc ctggacgacg
     3241 tcgtcctcgt cggaggagcc aacggtcttt ccgacgcgcc cgaggcgaat tctcgggccg
     3301 acgccgacgg agcccacgtc gcggatgcgg atcggggtgc cgttccgcgt gacgacggcg
     3361 atctcctcga tgtcggccgg ggactggacg aggccgagac cgcgcacgac gtacatctcc
     3421 gcgccgtgct cgatgtaggc gccccccgcg ttccggttcc ccttcgcgag cgcatcgaag
     3481 acctgaggca gcgtcaggcc gtacgatttc agcttgagcg ggtcgacgtt gacctggtac
     3541 tgcttcgtgg ggccgccgaa gccgacgacg tccacgacgc ccggcaccgt ccggagcctg
     3601 cgctcgacga cccagtcctc cagggacttc agctccagcg gtgagatgcc gggcgggccc
     3661 gtgaccgtgt atcggtagat ctcgccgacg ggcgtcgagt ccggcgagag gttcgcctgc
     3721 gcgccttggg ggagggcgac ggactggagg cgctcgaagg cctggttgcg gacgtaagcc
     3781 cggtccgcgt cgtcctcgaa gacgatcgtc acgacggaga gcccgaagat cgagatcgag
     3841 cgcatcgaag cgcgcttcgg gatcccgttc atctcgtttt cgacggggat cgtgacgaga
     3901 cgctcgacct cctcggccgc gtggccggcg aagagcgtga tgatctgcac ctggacgttc
     3961 gtgacgtccg gatacgcctc gacagggagg cgtttgaagg cgagcactcc agctgcgacg
     4021 aagacgacga gcagcgccag gacgatcacg cgccggcgca gcgaccatgc gacgagggcg
     4081 ccgatcactt cttttccccg gcggacgcga acagcgacga gaggagaagg ctgccgtccg
     4141 tcacgacgcg cgcgccttcg gggagcccgg cgagaaccgg gaccttgccc ccgcgctcgg
     4201 gcccgaccgt cacgcgcgtc cgccggaacc gcgacgggcc cgcctcgacg aagcagacgc
     4261 gcccggcggc gtccgtgagg atcgcgcgtg ccgggaccac cgtcgcgggc tgcgccgacc
     4321 tgccggaaac ctcgacggtg acgtacatct ccgccttgag gtggccctcg ggattgtcga
     4381 cggcgccgcg tgcccggacc gtccgcgtcg cggggtcgag cgcgtcaccc acgaggtcga
     4441 gccttcccgt gaagacgcgg cccggccagg cggccgagcg aatcgagagc cggtcgccgg
     4501 gcgcgaggag cgcgaggtcg cgctccgtga cgtcgagcca gacccagaga ctcttcgggt
     4561 tcgtcacgac gaagagggga gcggcggcgt ccggccgggc ttcctgcccg ggattcgcat
     4621 tgcgctcgac gacgacgccc ggcaggggag acccgagccg ga
//
LOCUS       DAOMFY010000303         4653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6623_length_4653_cov_8.860374, whole genome shotgun sequence.
ACCESSION   DAOMFY010000303 DAOMFY010000000
VERSION     DAOMFY010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6623_length_4653_cov_8.860374"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLB01_17330"
     CDS             complement(<1..296)
                     /locus_tag="PLB01_17330"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HQQ79115.1"
                     /translation="MTGAGRRAGGAAPASAAAPLVLYFDGASRGNPGPAAYGVWSPSG
                     LEKADVLGTTTNNVAEWRGFLAALDLAVASGAEDVEIRADSQLVLRQFSGEYRM"
     gene            complement(293..1027)
                     /locus_tag="PLB01_17335"
     CDS             complement(293..1027)
                     /locus_tag="PLB01_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQQ79116.1"
                     /translation="MSTGTMGALDRLFLLQEVLIEVQLKTEKRGQTPEHLAHIEAAYR
                     DARQQREETERTLSKAAERKKALEGEILDLNEKLKKYQGQLQTVKTNREYGALLNELD
                     VVKRDVRTREDEILALEEAVSVATAELERRNEAFPAEEAGYEEQMKEWRAEQAVLTAE
                     IEKAEAQARALRAELDARLLSTFDRLARVRAGIAVAKVDMVGTQTAACSACHVRLRPQ
                     LLSDLRLARETVTCESCKRILYWAGA"
     gene            complement(1190..1265)
                     /locus_tag="PLB01_17340"
     tRNA            complement(1190..1265)
                     /locus_tag="PLB01_17340"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1230..1232),aa:Ile,seq:cat)
     gene            complement(1387..3138)
                     /gene="rpoD"
                     /locus_tag="PLB01_17345"
     CDS             complement(1387..3138)
                     /gene="rpoD"
                     /locus_tag="PLB01_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HQQ79117.1"
                     /translation="MTVEEKHPEVRTLINLGKERGYLLYDEIYQALPEELANAPEELD
                     EIYIRFGDFGIEVVDVPEKATKKAAAPGTETTVPDPATVEKTNDPVRMYLREMGTVKL
                     LDREGEVSIARRIENGEAKIYRALSTNRVVLEEILRIVEQAKKDKNVARGFLEFAAAA
                     LEDADEIDPRTAHRIEGILGHFKKIAKLDGELRKLAEKKKPAPKAKKLTVKAMAQSRA
                     QHKAGGKPVPPTKPVPIVDPAIDRRISEIARLIHAIDFTPATLLRMVNILRDIDRQFA
                     MPEATIRQDLANIKKEKNEVRRDFYKRRVSKYRQMLKDLEEKYGVSHDEIKKTMRQIR
                     EGEDEADQAKQELIVANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVEKFEYRRG
                     YKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLTRTQRSLVQELGREPTAEE
                     IAKRMDMPASKVRKIMKIAQEPISLETPIGEEEDSHLGDFIEDRQVISPIDAVIVANL
                     KEQTRRTLKTLTPREEQVLRMRFGVGDGSEHTLEEVGKSFNVTRERIRQIESKALRKL
                     RHPSRAKKLRPFVDVGQ"
     gene            complement(3182..>4653)
                     /locus_tag="PLB01_17350"
     CDS             complement(3182..>4653)
                     /locus_tag="PLB01_17350"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HQQ79118.1"
                     /translation="EALAAAAAHFRANLEKHPAARALLEKRGLTPDEGAAFGFGAALD
                     SWDALKNGLGARFTEEALVDAGLLQKNPETGRVYDRFRNRLTVEIRDARGEILGFGAR
                     AFGDEQPKYLNSPETARFSKGKLLYGLDRARDAIRKEDAVLLVEGYFDRIACERAGVG
                     WAVASMGTALTPAQADLLSRHAATVVVAYDGDAPGVAAAFKAFPLLLARGANVKLLAL
                     PNGHDPDSFLAAHGAEAFRAEVAAAKPLLDALLAGIPPAGSDAVLRAKSLREAAEILS
                     AASDPVLRHELLTGLARGADVPLAVLAKGAPAVEGKKPAPPAVRKEAVASPQELPEQE
                     ARVLSVVLSDWPESAPLVGRIPGELFSHPIAREVFTALKEYASQPGTLDFSSLTTHLG
                     ADAGPVAAQLLLREGGPKAESGGTAGSEGLSRIHIPLLQLKIRLLEETAKNLQPEIQS
                     ASSAGDRPAGDAATRKKQDLVEEIRRLKAELKAETARRI"
BASE COUNT          624 a         1596 c         1559 g          874 t
ORIGIN      
        1 ttcatccggt actcgccgga gaactggcgc aggacgagct ggctgtccgc gcggatctcg
       61 acgtcctcgg cccccgaggc caccgcgagg tcgagcgccg cgagaaaacc gcgccattcg
      121 gccacgttgt tcgtcgtcgt tccgagcacg tcggccttct cgaggccgga gggagaccag
      181 acgccgtacg ccgcaggccc cggatttccc cgcgaggccc cgtcgaagta gaggacgagt
      241 ggagcggcgg cggaagcggg agcggcgccg cccgcgcgtc gccccgcgcc cgtcacgcgc
      301 ccgcccagta gaggatgcgc ttgcagctct cgcaggtcac ggtctcgcgg gccagccgga
      361 ggtccgacag cagctgcggc ctcaggcgca cgtggcaggc cgagcacgcg gccgtctggg
      421 tcccgaccat gtcgaccttc gcgaccgcga tccccgctcg aacgcgcgcg agccggtcga
      481 acgtggacag gagccgggcg tcgagctcgg cgcgcagggc ccgcgcctgc gcctcggcct
      541 tctcgatctc cgcggtgagg accgcctgct cggcgcgcca ctccttcatc tgctcttcgt
      601 agcccgcttc ctcggccggg aacgcctcgt tccgcctctc gagctcggcc gtcgccaccg
      661 acacggcctc ctcgagggcg aggatctcgt cctcgcgcgt cctgacgtcg cgcttcacga
      721 cgtcgagctc gttcaggagg gcgccgtact cgcggttcgt cttgaccgtc tggagctgac
      781 cctggtactt cttgagcttc tcgttcaggt cgaggatctc gccttcgagc gccttcttgc
      841 gctcggccgc cttcgagagc gtccgctcgg tctcctcgcg ctgctggcgc gcgtcgcggt
      901 acgccgcttc gatgtgcgcg aggtgctccg gcgtctgccc gcgcttctcg gtcttcagct
      961 ggacctcgat caggacttcc tgaaggagaa acagccggtc cagtgccccc atggtgcccg
     1021 tgctcaacgt cccgctccca tctctcgtgg tgtcacgaag aaacccctga aaacgaaaaa
     1081 cgggcgccgc acggcgcccg cctggacgaa cccccggcgc tcgaaagcgc ccgcgggatc
     1141 ggaatgaatt cgtcgcgacc ggattttcga tctccctctg ccgccgacct ggtgggtccg
     1201 cctggattcg aaccagggac ccgccggtta tgagccggtg gctctgaccg ctgagctacg
     1261 gaccccgacg gccgaaaaag gagaatagca cgacggggat tttcttagaa ggcgcgtgcg
     1321 gcagccgtcc gcgggcatgc ggctcgccgc gactctttcg gccctcttac cttctaagaa
     1381 atcttcttac tggcccacat caacaaaggg acgcagcttc ttcgcccggc tggggtgccg
     1441 gagcttccgg agcgccttgc tctcgatctg ccggatgcgc tcgcgggtga cgttgaagct
     1501 cttgccgacc tcctcgagcg tgtgctcgga gccgtcgccg acgccgaagc gcatgcggag
     1561 gacctgctcc tcgcgcggcg tgagggtctt gagcgtgcgg cgcgtctgct ccttgaggtt
     1621 cgcgacgatc acggcgtcga tcggcgagat gacctggcgg tcctcgatga agtcgccgag
     1681 gtgcgaatcc tcctcctcgc cgatgggcgt ctcgagggag atgggctcct gcgcgatctt
     1741 catgatcttg cgcaccttcg aggccggcat gtccatgcgc ttggcgatct cctcggccgt
     1801 cggctcgcgg ccgagctcct ggacgagcga gcgctgcgtg cgcgtgagct tgttgatcgt
     1861 ctcgatcatg tggaccggga tgcggatcgt gcgcgcctgg tccgcgatcg cgcgcgtgat
     1921 ggcctggcgg atccaccacg tcgcgtacgt cgagaacttg tagccgcggc ggtactcgaa
     1981 cttctcgacg gccttcatga ggccgatgtt cccctcctgg atgaggtcga ggaactggag
     2041 gccgcggttc gtgtacttct tcgcgatcga gacgacgagc cggaggttcg cgacgatgag
     2101 ctcctgcttg gcctgatcgg cctcgtcctc gccctcgcgg atctgccgca tcgtcttctt
     2161 gatctcgtcg tgcgagacgc cgtacttctc ctcgagatcc ttcagcatct gccggtactt
     2221 ggagacgcgg cgcttgtaga agtcgcggcg gacctcgttc ttctccttct tgatgttcgc
     2281 gaggtcctgc cggatcgtgg cctcgggcat cgcgaactgc cggtcgatgt cccggaggat
     2341 gttgaccatc cggagcagcg tcgcgggcgt gaagtcgatc gcgtggatga ggcgcgcgat
     2401 ctcggaaatg cggcggtcga tcgccgggtc gacgatgggc acgggtttcg tcggcgggac
     2461 cggctttccg cccgctttgt gctgcgcgcg cgactgcgcc atcgccttga ccgtgagctt
     2521 cttcgccttc ggcgcgggct tcttcttctc ggccagcttg cgcagctcac cgtcgagctt
     2581 cgcgatcttc ttgaagtgcc cgaggattcc ctcgatgcgg tgcgccgtcc gcgggtcgat
     2641 ctcgtcggcg tcctcgaggg ccgcggccgc gaactcgagg aacccgcgcg cgacgttctt
     2701 gtccttcttg gcctgctcga cgatccggag gatctcctcg agcacgacgc ggttcgtcga
     2761 cagcgcgcgg tagatcttcg cctcgccgtt ctcgatgcgc cgcgcgatgg aaacctcgcc
     2821 ttcgcggtcg aggagcttca cggtgcccat ctcgcgcagg tacatgcgca cggggtcgtt
     2881 cgtcttctcg acggtggccg ggtcggggac ggtcgtctcc gtgcccggcg ccgcggcctt
     2941 cttcgtggcc ttctccggga cgtcgacgac ctcgattccg aagtcgccga agcggatgta
     3001 gatctcgtcg agctcctccg gagcgttcgc gagctcctcg ggcagcgcct ggtagatctc
     3061 gtcgtacagg agatacccgc gctccttgcc gaggttgatc agcgtccgga cttcggggtg
     3121 cttttcttct acggtcaccg gtgtctcact tccatgcgct ccggcctctc cggggcgctc
     3181 ctcagattcg tctcgcggtc tcggctttga gttccgcctt caggcggcgg atttcctcga
     3241 ccaggtcctg tttctttctc gtcgccgcgt cccccgccgg tcgatctccg gctgacgagg
     3301 cgctctggat ctccggctgg aggtttttcg cggtctcctc caggaggcgt atcttcaact
     3361 gcaacagtgg gatgtgaatg cggctgagcc cctccgagcc cgccgtgccc ccgctctcgg
     3421 cttttggacc gccctcccgc aggaggagtt gagcggccac gggacccgca tcggccccaa
     3481 gatgtgtcgt aagtgacgaa aaatcaaggg ttcccggctg agacgcatac tcttttaggg
     3541 ctgtaaagac ttcccgcgca atcggatgtg aaaaaagctc gcccggaatc cgtcccacga
     3601 ggggggcgct ctccggccag tcggaaagaa cgacggaaag aacccgggcc tcctgttccg
     3661 gtagctcctg tggtgacgct accgcctctt ttctaactgc tggaggcgcc ggtttcttcc
     3721 cttcgaccgc gggggcgccc tttgcgagca ccgcgagcgg cacgtccgcc cctcgtgcga
     3781 ggccggtcag gagctcgtgg cggaggaccg ggtcggacgc cgccgagagg atttcggcgg
     3841 cctcccgcag cgatttcgcc cgcaggaccg cgtcgctccc ggccggggga atccccgcga
     3901 ggagggcgtc gagaagcggt ttcgccgcgg ccacctcggc gcgaaacgcc tcggcgccgt
     3961 gcgcggcgag gaacgaatcg gggtcgtggc cgttcggcag cgccaggagc ttcacgttcg
     4021 cgcctcgggc gaggaggagc gggaacgcct tgaaggccgc ggctacgccg ggcgcgtcgc
     4081 cgtcgtacgc gacgaccacc gtcgcggcgt gccgcgacag gaggtccgcc tgggccggcg
     4141 tgagcgcggt gcccatcgac gcgacggccc acccgacgcc ggccctctcg cacgcgatgc
     4201 ggtcgaagta accctcgacg agaagcacgg cgtcttcctt ccggatggcg tcgcgcgcac
     4261 ggtcgaggcc gtagaggagc ttgcccttcg agaagcgcgc cgtctcgggc gagttgaggt
     4321 acttcggctg ttcgtcgccg aaggcgcgcg cgccgaagcc gaggatctcg ccgcgtgcgt
     4381 cccggatctc gaccgtgaga cggttgcgga accggtcgta gacgcggccc gtctcggggt
     4441 tcttctgcag gagacccgcg tcgacgagcg cctcctccgt gaagcgcgcg ccgaggccgt
     4501 tcttgagcgc gtcccacgaa tcgagcgcgg cgccgaatcc gaaggccgct ccctcgtcgg
     4561 gcgtcaggcc gcgcttttcg agaagcgccc gcgcggccgg gtgcttctcg aggttcgcgc
     4621 ggaagtgcgc cgccgccgcc gcgagcgcct cgg
//
LOCUS       DAOMFY010000304         4649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6631_length_4649_cov_6.347410, whole genome shotgun sequence.
ACCESSION   DAOMFY010000304 DAOMFY010000000
VERSION     DAOMFY010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6631_length_4649_cov_6.347410"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /gene="radA"
                     /locus_tag="PLB01_17355"
     CDS             <1..1307
                     /gene="radA"
                     /locus_tag="PLB01_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQQ79119.1"
                     /translation="GAQSPQWIGRCPECGGWNTLAEEREAPRTPAAGAASSAVRLSDV
                     AAAEVPRLPTGMADVDRVLGGGLVPGSIVLLGGEPGIGKSTLLLQVASRLSDRIGEVL
                     YVSAEESAQQIRMRAVRLGVAAPGLFLLAETNLDAILEAARTGRYAAVVVDSIQTIAS
                     PAVQAAAGSVTQVRECAGRLQAFAKTTGVPVFLIGHVTKDGTLAGPKTLEHLVDAVLS
                     FEGERFHAQRVVRALKNRFGPAHELGVFEMTGSGLVEVANPSALYLGTGAGSRPGSVV
                     LAGVEGTRPLLLEVQALTVEAKYGSPRRTAIGFDGTRLALLLAVLERHGGLKLASHDV
                     FLNVAGGAESVEPAADLAVCAAVAGSLRGRALAPGTFAFGEVGLLGEVRAVSDAALRL
                     KEAVSLGLKTALVPAGNAGEAAALPDLSVVPLRSVEELLART"
     gene            complement(1311..1508)
                     /locus_tag="PLB01_17360"
     CDS             complement(1311..1508)
                     /locus_tag="PLB01_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79120.1"
                     /translation="MAKFLLWCILFVLCWPLAILALIAFPFVWLLLLPFKVVGIAVGG
                     VLAFLKSLLFLPARLLAGRPV"
     gene            1620..2141
                     /locus_tag="PLB01_17365"
     CDS             1620..2141
                     /locus_tag="PLB01_17365"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQQ79121.1"
                     /translation="MTKTLDLSKLDLQDALDLAILVEDEAKERYDELAAQMETHHTAE
                     AAEFFHFMSKNEAKHGEELAARRKELFGDAARRVDRSMVWEIEAPEYENVHAFMTLHE
                     ALTVAMAAEVKAHWFFTDALKLPMADPVRKLFLELQEEEVHHQELVGREIAKLPPDEA
                     APPEEFADEPVSQ"
     gene            complement(2157..3053)
                     /locus_tag="PLB01_17370"
     CDS             complement(2157..3053)
                     /locus_tag="PLB01_17370"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQQ79122.1"
                     /translation="MPEAVLETSLPELPPPRRGKVRDVYDLGDRLLLVATDRISAFDC
                     VLSPGIPDKGKILTQLSNFWFRRFAEVVPNHLIETDASRFPEPLAAHAALLSGRSVLA
                     KKCAVVPFECVARGYLAGSGWKEYRESGAVCGIPLPKGLREADRLPDAIFTPATKAES
                     GHDINVSFEAMVSATGLELATRLKSLTLELYSRAAAYAAGRGIVLADTKFEFGLDPSG
                     GVVWIDEVLTPDSSRFWPADTWRPGGSPPSFDKQFVRDFLEKTGWNKTPPAPVLPPDV
                     VAGTRARYAEAYERLTGMPFRA"
     gene            3127..>4649
                     /locus_tag="PLB01_17375"
     CDS             3127..>4649
                     /locus_tag="PLB01_17375"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQQ79123.1"
                     /translation="MLKLAIRTRKGGARADAFVAFAVGATPKEALAGLPAAVRAKGAA
                     AAPAQPAAEGGVFTAPLGGSPAGRLYVFGGGKSAEISPRKARAILRSVAKTLNRSGER
                     TAILDFPFRLKRMDAAETREFLLRALALADYAFPVYKTKDREKRTLASVAVQATGAFA
                     GATAAEARDAEALAKVAAFVRDLGNTPSNDMVPRDFARRVAEEARARGLRAKVLDKKA
                     IRREKMGGLLAVNRGSAEEPRVVVLEWKGGKKGEAPLAFVGKGVTFDSGGISIKPSEK
                     MGDMKWDMMGAATAMGIVLAAADLKLPVNVVGIAALTENLPSGTAYKPGDIVRFRNGK
                     TAEIDNTDAEGRVILADALDYAADWKPAAILDFATLTGAALIALGLEAAIVFTDHDDL
                     SRALVSAGERTDERIWRLPMWEEYKENIRSDWADMKNTGGRFGGSINGAMFLKEFVDP
                     KTPWAHLDIAATANVDREYAGYPVGATAFGVGLALRWMRDRAAGARGAVASLAPETQR
                     "
BASE COUNT          706 a         1602 c         1722 g          619 t
ORIGIN      
        1 gcggagccca gagcccgcag tggatcgggc gctgccccga gtgcggcggg tggaacacgc
       61 tggcggagga gcgcgaggcg ccgcgcacgc ccgcggccgg cgccgcgtcc tcggcggtgc
      121 gcctgtccga cgtcgccgcg gcggaagtcc cgcggctccc gacgggcatg gccgacgtgg
      181 accgcgtcct cggcggcggc ctcgtgccgg gctcgatcgt cctcctcggc ggcgagccgg
      241 ggatcggcaa gtcgacgctg ctcctccagg tggcctcccg cctgtccgat cggattggcg
      301 aagttctcta cgtcagtgca gaagagtccg cgcagcagat ccggatgcgc gccgtgcgcc
      361 tcggggtggc cgcgcccggg ctcttccttc tcgccgagac gaacctcgac gcgatcctcg
      421 aggcggcgcg cacgggtcgc tacgcggcgg tcgtcgtgga ctcgatccag acgatcgcct
      481 cgccggccgt gcaggcggcg gcggggtccg tgacgcaggt gcgcgagtgc gcgggccggc
      541 tgcaggcgtt cgcgaagacg acgggcgtcc ccgtgttcct gatcgggcac gtcacgaagg
      601 acgggacgct ggccggaccc aagacgctcg agcacctcgt ggacgcggtc ctctcgttcg
      661 agggcgagcg cttccacgcg cagcgtgtcg tgcgcgcgct caagaaccgc ttcgggccgg
      721 cgcacgagct cggcgtcttc gagatgacgg gttcgggtct cgtcgaggtc gcgaatccgt
      781 ccgcgctcta cctcggcacg ggcgcgggct cgcggccggg ctcggtcgtc ctcgcgggag
      841 tggagggcac gcgtccgctg ctgctcgaag tccaggcgct gacggtggag gccaagtacg
      901 gctcgccccg aaggacggcg atcgggttcg acggcacgcg cctcgcgctc ctgctcgcgg
      961 tcctcgagcg ccacggcggg ctgaagctcg cgtcgcacga cgtcttcctg aacgtggcgg
     1021 gcggagccga gagcgtggag ccggctgccg acctcgccgt gtgcgccgcg gtcgcgggca
     1081 gcctgcgcgg ccgggccctc gcgcccggga cgttcgcgtt cggcgaggtc ggccttctcg
     1141 gcgaagtgcg cgccgtctcc gacgcggcct tgcgcctcaa ggaagccgtc tcgctgggcc
     1201 tgaagaccgc cctcgtcccc gcaggaaacg cgggggaggc ggccgccctt ccggacctct
     1261 cggtcgtccc gctccggagc gtcgaggaat tgctggcccg gacctgagcc tcagaccggc
     1321 cttccggcga ggaggcgggc cgggagaaac aggagcgact tcaggaacgc gagcacgccg
     1381 ccgacggcga tcccgacgac cttgaagggg aggaggagga gccagacgaa cgggaaggcg
     1441 atcagggcga ggatcgccag cggccagcag aggacgaaga ggatgcacca gagcaggaac
     1501 ttcgccatcg aggcctcccg caactgatac ggacccgccg gaccggggtt tccggttgat
     1561 ccggttgcat cattttgcgc ccggtgtctt ccatgcgagc gtagcctccg gagaaccgta
     1621 tgacgaagac gctcgacctg tcgaagctcg accttcagga cgccctcgac ctcgccatcc
     1681 tcgtggagga cgaagccaag gagcgctacg acgagctcgc cgcgcagatg gagacgcacc
     1741 acacggccga ggcggccgag ttcttccact tcatgtcgaa gaacgaagcc aagcacggcg
     1801 aggagctcgc cgcccgccgc aaggagctct tcggcgacgc cgcccggcgg gtggaccgct
     1861 cgatggtgtg ggaaatcgag gccccggagt acgaaaacgt ccacgcgttc atgacgctcc
     1921 acgaggccct gacggtcgcg atggcggccg aggtgaaggc ccactggttc ttcacggacg
     1981 cgctgaagct cccgatggcc gacccggtcc ggaagctttt cctcgagctg caggaggaag
     2041 aggtccacca ccaggagctc gtcggccgcg agatcgcaaa gctcccgccc gacgaggccg
     2101 cacccccgga agagttcgcg gacgagccgg tctcccagta gagggttcgc gcccggtcag
     2161 gccctgaacg gcatcccggt caggcgctcg tacgcctcgg cgtagcgcgc gcgtgtcccc
     2221 gcgacgacgt cgggcggcag gacgggcgcg ggcggcgtct tgttccaccc cgtcttctcg
     2281 aggaagtcgc ggacgaactg cttgtcgaag gacgggggcg atccgcccgg gcgccacgtg
     2341 tccgccggcc agaaacggga ggagtcggga gtgagcacct cgtcgatcca gacgacgccc
     2401 ccggacgggt cgaggccgaa ctcgaacttc gtgtccgcga ggacgatccc gcgcccggcc
     2461 gcgtacgcgg cggcacgcga gtagagctcg agcgtgaggc tcttcaggcg cgtcgcgagc
     2521 tcgagccctg tcgccgaaac catggcctcg aacgagacgt tgatgtcgtg cccggactcg
     2581 gccttcgtgg cgggcgtgaa gatcgcgtcg ggaaggcggt ccgcttcacg cagccccttc
     2641 ggcagcggga tgccgcagac ggcgcccgac tcgcggtact ccttccagcc ggagcccgcg
     2701 agatagccgc gggccacgca ctcgaacggg acgacggcgc acttcttcgc gaggacgctg
     2761 cggcccgaga ggagcgcggc gtgcgcggcg aggggctcgg ggaaccgcga ggcgtccgtt
     2821 tcgatcaggt ggttcgggac gacctcggcg aaccggcgga accagaagtt cgagagctgc
     2881 gtgaggatct ttcccttgtc gggaatgccg ggggagagca cgcagtcgaa ggcggagatg
     2941 cggtcggtcg cgacgaggag gaggcggtcg ccgaggtcgt agacgtcccg cacctttccg
     3001 cggcgcggcg gcgggagctc cggcagggaa gtctcgagga cggcttctgg catgcgccgg
     3061 attctaggtc gtagacgccg ggcggcgcgc tagactttgg ctcccccagg ggaccaggag
     3121 gacactttgc tcaagcttgc gattcgaacc cggaagggtg gtgcgcgcgc ggacgccttc
     3181 gtcgcgttcg cggtcggcgc gaccccgaag gaggcgctcg cggggctgcc tgccgccgtg
     3241 cgcgcgaagg gagccgcggc cgctccggca cagcccgcgg ccgagggcgg cgtgttcacg
     3301 gcgccgctgg gaggctcacc ggccggccgg ctttacgtgt tcggaggcgg caaaagcgcc
     3361 gagatcagcc cgcgcaaggc gcgggcgatc ctccgcagcg tcgcgaagac gctgaaccgc
     3421 agcggcgagc ggaccgcgat cctcgacttc ccgttccggc tcaagcggat ggacgccgcc
     3481 gagacgcggg agttcctcct ccgcgcgctc gcgctggcgg actatgcgtt ccccgtctac
     3541 aagacgaagg accgcgagaa gcggaccctc gcgtccgtgg ccgtccaggc gaccggcgcg
     3601 ttcgcggggg ccacggccgc cgaggcgcgc gacgccgagg cgctcgcgaa ggtcgccgcg
     3661 ttcgtgcgcg acctcgggaa cacgccctcg aacgacatgg tgccccgcga cttcgcccgc
     3721 cgcgtcgccg aggaggcccg cgcgcgcggc ctccgggcga aggtcctcga caagaaggcg
     3781 atccgccgcg agaagatggg cggcctcctc gccgtgaacc gcggctcggc cgaggagccg
     3841 cgcgtcgtcg tcctcgagtg gaagggcggc aagaagggcg aggcgccgct cgcgttcgtc
     3901 ggcaagggcg tcacgttcga ctccggcggc atctcgatca agccctccga gaagatgggc
     3961 gacatgaagt gggacatgat gggcgccgcc accgcgatgg ggatcgtcct cgcggccgcg
     4021 gacctgaaac tgcccgtcaa cgtcgtcggg atcgccgccc tcacggagaa cctcccgtcc
     4081 gggacggcct acaagccggg cgacatcgtc cgcttccgca acgggaagac cgccgagatc
     4141 gacaacacgg acgccgaggg gcgcgtgatc ctggcggacg cgctcgacta cgccgccgac
     4201 tggaagcccg ccgccatcct cgatttcgcg acgctcacgg gcgcggcgct gatcgcgctc
     4261 ggcctcgagg ccgcaatcgt gttcacggac cacgacgacc tctcgcgcgc gctggtctcg
     4321 gccggcgagc ggacggacga gcgcatctgg cgccttccga tgtgggagga gtacaaggag
     4381 aacatccggt ccgactgggc cgacatgaag aacacgggcg gccgcttcgg cgggtcgatc
     4441 aacggggcga tgttcctcaa ggagttcgtc gacccgaaga ccccgtgggc gcacctcgac
     4501 atcgcggcga ccgcgaacgt cgaccgcgag tacgccggct acccggtcgg cgcgaccgcg
     4561 ttcggcgtgg gcctcgccct gcggtggatg cgggaccgcg ccgccggggc gcgcggtgcc
     4621 gtcgcctccc tcgccccgga aacgcagcg
//
LOCUS       DAOMFY010000305         4642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6644_length_4642_cov_6.885546, whole genome shotgun sequence.
ACCESSION   DAOMFY010000305 DAOMFY010000000
VERSION     DAOMFY010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6644_length_4642_cov_6.885546"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLB01_17380"
     CDS             complement(<1..540)
                     /locus_tag="PLB01_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79124.1"
                     /translation="MAAGPARASRLPDAAALAVLCTAFAARFHELVTGGTLYRRDAGF
                     FFVPWRSLLARFIAAGEWPLWNDWTSGGRPFAADPNAAVFWPLSPLVAVVGPTGLSLA
                     NTALVLLLFYWALRRFGLSAPGSAAGALVLLFSGVFQSLPGFFGMAAAAAPLPLAFAE
                     LPRLAAGDPGTRRRALALCA"
     gene            complement(546..1949)
                     /locus_tag="PLB01_17385"
     CDS             complement(546..1949)
                     /locus_tag="PLB01_17385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ79125.1"
                     /translation="MKLSIVMPAYNEKRTIREIVSRVKAVDLAGLEREIVIVDDGSKD
                     GTRDILREMDGQDGVRVVLQPHNMGKGAAVWTGMKASTGDIVIIQDADLEYDPREYPV
                     VLGPILKGEADIVYGSRFLGHPGGHRVLYFWHTVGNRVLTLMSNAFTNLNLTDMETCY
                     KAMRREIVDRLDLRSQRFGIEPEITCKVSRMRARIFEVPISYHGRTYEEGKKIGIKDG
                     FQAVWTILKYFRWEAPEGDVGAITLRRMEGLSVYNKWLHDRFEAWLGNRVLEVGSGVG
                     NQTRFFADKERVVASDIEPHYVGELKAKFEGRPNVRVASFRFPLADADARDLAAERVD
                     TIVCLNVLEHIEDDAATLRDFARVLPSGGRLALLVPSLPALYGTLDEALLHYRRYEKA
                     GLERLVAESGFAVEKIRYLNRPGVFGWWLNSRVLRRRVLPRQQLGAFKWLMPLLRAEE
                     DTEPSFGMSLLVLARKP"
     gene            complement(1946..3682)
                     /locus_tag="PLB01_17390"
     CDS             complement(1946..3682)
                     /locus_tag="PLB01_17390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ79126.1"
                     /translation="MQSPGPLRFWLGAAALGILGVLLVFDHARLDSPTMDEPFHTLAA
                     AEYAIAGTYNANLEHPPLVKLLSGFAVRAAGARPPRMDEPFSMRTAEQPRPFAFSSTL
                     TPERLFGAARAPVAALFFVLVLAVGGAVRHWGGSVAGLLAAALVALEPNLVAHAGVVH
                     TDLAAALGFFATLALCLLALEKRSLTLWAGAGAALGASLAAKFSCVLLVPMVAVLALV
                     GLFRERRAAAADGRRASALPAIGLLVAGAVSAAVLLGCYAVAMRNMSPRDAEGAIRIF
                     LASREAPPAAVERVVALSRVSKQAGHYAAGLAGIAIQNRAGGGVNFLCGKLSVDGFWN
                     YFFVAFAVKSALGFLGVLGAAVVLAAAKRDRLTLVIWALVLPAAYLFLTGMATSYNIG
                     IRHMLPVYPLLAAAAVLAVFRALPPRAAAAVLTAGVAIQAAEILAVHPHELSFFNAAA
                     GGPARGAAWLNDSNLDWGQDLARVAMRLKARGEDSGTTVAYFGGADPAYYLPKARPFE
                     GPLDPLAPGRYAVSEFIRCCGPEAMAFHGDAAGAAGFERLRRALRERGTPLAREGMGY
                     SVILWELKEKSP"
     gene            3742..>4642
                     /locus_tag="PLB01_17395"
     CDS             3742..>4642
                     /locus_tag="PLB01_17395"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HQQ79127.1"
                     /translation="MQTRARGRDGLFVALAFLAALAFRFAPFPDATAHGWRLLSPDCY
                     GHLRRTVSVARNFPRVPYRDPYLNHPDGGVFIWPPAFDLLAGGISRALYGASATTEQV
                     TRVAAALPAVLGALHVLPLFFLARRVFGRRRARIAAAAYVAIPAAVLWGGFGHFDHHV
                     AEALNLLLVLAAGAWAAAAPPGEKLARSAAFGAAIALAVLTWQGAVFVAGLGLVLFAL
                     LILAWAAEPVVFDALTYRLSRIGAWLQDGRIGVLATDDPRLNYMPVAPDIVIAWLLGS
                     TAVGFRGAPVAQLLGGCLLLGATF"
BASE COUNT          688 a         1619 c         1722 g          613 t
ORIGIN      
        1 cgcgcagagc gcaagggccc ggcggcgcgt tccgggatcg ccggcggcga ggcgcggcag
       61 ctcggcgaac gcgaggggca gcggcgcggc ggcggccgcc atcccgaaga agccgggcag
      121 cgactggaag acgccggaga acagaaggac gagcgcgccc gccgcggagc ccggcgccga
      181 caggccgaac cggcgcagcg cccagtagaa gaggaggagc acgagcgccg tgttcgcgag
      241 ggagaggccc gtcggcccga cgaccgcgac gagcggcgag agcggccaga acacggcggc
      301 gttcgggtcg gcggcgaagg gccgtccgcc cgacgtccag tcgttccaga ggggccactc
      361 gccggcggcg atgaagcgcg cgagcaggct ccgccacggc acgaagaaaa agcccgcgtc
      421 gcgcctgtag agcgtcccgc cggtcacgag ctcgtgaaac cgcgccgcga acgcggtgca
      481 aaggacggcg agggccgcgg cgtccggcag ccgggaggct ctggccggtc ccgccgccat
      541 cgacgtcagg gcttccgggc gaggacgagg agcgacatcc cgaaggacgg ttccgtgtcc
      601 tcctcggccc tgaggagcgg catgagccac ttgaacgcgc cgagctgctg ccgcgggaga
      661 acccggcggc ggaggacgcg cgagttcagc caccatccga agacgcccgg ccggttcagg
      721 taacggatct tctccacggc gaaaccggac tcggccacga gccgctcgag gccggccttt
      781 tcgtaccggc ggtagtggag cagagcttcg tcgagcgtcc cgtagagagc cggcaaggag
      841 gggacgagga gcgcgaggcg cccgccggag ggcaggacgc gggcgaaatc gcgaagggtg
      901 gccgcgtcgt cctcgatgtg ctcgagaacg ttcaggcaga cgatcgtgtc gacgcgctcg
      961 gcggcgaggt cgcgggcgtc tgcgtccgcg agcgggaagc ggaacgacgc gacgcggacg
     1021 ttcgggcggc cctcgaactt cgccttgagc tcgccgacgt agtgcggctc gatgtcggag
     1081 gcgacgacgc gttccttgtc ggcgaagaag cgcgtctggt tcccgacgcc ggagccgacc
     1141 tcgaggacgc ggttcccgag ccacgcctcg aagcggtcgt gcagccactt gttgtagacg
     1201 gagagcccct ccatccggcg gagcgtgatc gcgccgacgt cgccctcggg cgcctcccac
     1261 cggaagtact tgaggatcgt ccagacggcc tggaagccgt ccttgatccc gatcttcttg
     1321 ccctcctcgt acgtgcggcc gtggtacgag atcggcacct cgaagatgcg cgcgcgcatg
     1381 cgggagacct tgcacgtgat ctcgggctcg atcccgaagc gctgggaccg gaggtccagc
     1441 cggtccacga tctcgcggcg catggccttg tagcaggtct ccatgtccgt caggttgagg
     1501 ttcgtgaacg cgttcgacat gagcgtcagg acgcggttcc cgaccgtgtg ccagaagtac
     1561 agaacacggt gcccgccggg gtggccgagg aagcgcgagc cgtagacgat gtcggcctcg
     1621 cccttgagga tcgggccgag gacgacgggg tactcgcgcg ggtcgtactc gaggtccgcg
     1681 tcctggatga tcacgatgtc gcccgtggac gccttcatgc ccgtccacac cgccgcgccc
     1741 ttccccatgt tgtggggctg gaggacgacc cgcacgccgt cctgcccgtc catctcccgc
     1801 aggatgtcgc gcgtcccgtc cttcgagccg tcgtcgacga ttacgatctc gcgctcgagg
     1861 ccggcgaggt cgacggcctt cacgcgtgag acgatctcgc ggatcgtcct cttttcgttg
     1921 taggccggca tcacgatcga gagtttcacg ggctcttttc cttcagctcc cagaggatga
     1981 cggagtatcc catcccttcg cgcgcgagcg gcgtgccgcg ctctctcagc gcgcgccgga
     2041 ggcgctcgaa gccggccgca ccggccgcgt cgccgtggaa cgccatggcc tccggcccac
     2101 agcaccggat gaactccgac acggcgtagc ggccgggcgc gagcgggtcg agggggccct
     2161 cgaacggccg cgccttcgga aggtagtacg cggggtccgc gccgccgaag taggcgaccg
     2221 tcgttcccga gtcctccccg cgcgccttga gccgcatcgc gacgcgcgcg aggtcctgcc
     2281 cccagtcgag gttcgagtcg ttcagccacg cggccccccg cgccgggcct cccgcggccg
     2341 cgttgaagaa cgagagctcg tgcgggtgga ccgcgaggat ctcggcagcc tggatcgcga
     2401 caccggccgt cagtacggcg gcggcagcgc gcggcggcag cgctcggaag acggcgagaa
     2461 cggccgccgc cgcgaggagc ggatagacgg ggagcatgtg gcggatcccg atgttgtacg
     2521 acgtcgccat gccggtcaga aagaggtacg cggccggaag cacgagcgcc cagatgacga
     2581 gcgtgaggcg gtcgcgcttc gcggccgcga ggacgacggc ggcgcccaga actccgagga
     2641 agccgagtgc cgacttcacc gcgaaggcta cgaaaaagta attccagaag ccgtcgacgg
     2701 agagcttccc gcacaggaag ttcacgccgc cgccggccct gttctggatc gcgattcccg
     2761 cgaggccggc ggcgtaatgg cccgcctgct tcgagacgcg cgagagcgcc acgacgcgct
     2821 ccaccgcagc cggcggtgcc tcgcgcgagg cgaggaagat ccggatggct ccctcggcgt
     2881 cgcgggggga catgttccgc atcgcgacgg catagcaccc gaggaggacg gcggccgaga
     2941 cggcgcccgc gacgaggagg ccgatcgcgg ggagggcgga cgccctccgc ccgtccgccg
     3001 cggccgcacg ccgctcccgg aagaggccga cgagagcgag gaccgcgacc atcggtacga
     3061 gcaggacgca ggagaacttc gccgcaaggc tcgcaccgag cgccgcgccc gcgcccgccc
     3121 agagcgtgag ggagcgcttc tcgagcgcga gaaggcacag ggcgagggtc gcgaagaaac
     3181 cgagcgccgc cgcgagatcg gtgtggacga cacccgcgtg cgccacgagg ttcggctcga
     3241 gggccacgag cgccgccgcg aggagcccgg cgacgcttcc gccccagtgg cgcacggcgc
     3301 cgcccacggc gaggacgagc acgaagaaga gcgcggcgac gggcgcgcgc gcggcgccga
     3361 acaggcgctc cggcgtcagc gtcgagctga aggcgaacgg gcgcggctgc tcggccgtgc
     3421 gcatcgagaa gggctcgtcc atgcgcggtg ggcgcgcgcc cgctgcgcgc accgcgaaac
     3481 ccgacaggag cttcacgagc ggagggtgct cgaggttcgc gttgtacgtc ccggcgatcg
     3541 cgtactcggc ggccgcgagc gtgtggaacg gctcgtccat cgtcgggctg tcgaggcgcg
     3601 catggtcgaa gacgaggagg acgccgagga tcccgagcgc ggcggcgccg agccagaacc
     3661 ggagcggccc gggagactgc atcccgcgat tgtagggggg ccggagcgga aggatcgcgg
     3721 acgggctgct acgctcgcgc cgtgcagacg cgcgcgcgcg gccgggacgg cctcttcgtg
     3781 gccctcgcct tcctcgccgc cctcgcgttc cgattcgcgc cgttccccga cgcgacggcg
     3841 cacgggtggc gactcctctc ccccgactgc tacgggcacc tccggcgcac ggtctcggtc
     3901 gcgcgcaact tcccgcgcgt cccgtaccgc gacccgtacc tgaaccaccc cgacggcgga
     3961 gtcttcatct ggccgcccgc gttcgacctc ctcgcgggag ggatctcgcg cgccctctac
     4021 ggagcgtccg cgacgacgga gcaggtgacg cgggtcgcgg ccgcgctccc cgcggtcctc
     4081 ggcgccctcc acgtcctccc cctcttcttc ctcgcgcggc gggtgttcgg acgccgccgc
     4141 gcccggatcg cggccgccgc gtacgtcgcg atcccggccg ccgtcctgtg gggcggtttc
     4201 gggcacttcg accatcacgt tgccgaggcg ctcaacctcc tgctcgttct cgcggccggc
     4261 gcgtgggccg cggccgcgcc gcccggcgag aagctcgcgc gctcggcggc attcggggcc
     4321 gcgatcgccc tcgccgtcct cacgtggcag ggcgccgtct tcgtggcggg cctcggtctc
     4381 gtgcttttcg cgctgctcat cctcgcgtgg gcggcggagc ccgtggtctt cgacgcgctg
     4441 acttatcggc tctcgcgaat cggcgcgtgg ctgcaggacg ggcggatcgg ggtgctggcg
     4501 accgatgacc cgcgcttgaa ttacatgccg gtggcgccgg acatcgtgat cgcgtggctg
     4561 ctgggttcca cggcggtcgg cttccggggt gcgccggttg cgcaattgct gggtggatgc
     4621 ctgctgttgg gcgcgacctt cg
//
LOCUS       DAOMFY010000306         4608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6733_length_4608_cov_9.029212, whole genome shotgun sequence.
ACCESSION   DAOMFY010000306 DAOMFY010000000
VERSION     DAOMFY010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6733_length_4608_cov_9.029212"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="PLB01_17400"
     CDS             <1..1781
                     /locus_tag="PLB01_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQQ79128.1"
                     /translation="RREASVVASRLDDAPPTAVVRVVSRDEIDALPGAHTVPDVLRSV
                     LGVDVRRRGPGGVQADVGIRGGDFNGTLVLVDGQPVNDPQSNHLSFDLDVPVAAIERI
                     EVLAGPGSSVWGSNPVGGAVNIVTRGGALGRAAVQLEGRYAHGTQSLDQGGARLAVRV
                     AEPVTVGVDWWRAETAGFRDDTEAATDILRLSSRWETGKGPVTLGLGIAERRFGAYAF
                     YGTAFPNQQESTRTRTATLGGTLAFGGFTLTPSVFVRAHHDDFVLDRGNPAFYENLSD
                     TVTSTARLSVSRAVLGGSVAAGVEGGRDTIASSNLGEHARDRGAIFAEFARSWSVADP
                     GFGGVRVGLRADTYDEYGSRVSPYAGLTVRAAPPLTLRASFGTSFRVPTFTELYYRDP
                     QSAGNPGLAPETAWSVDAGVKLAAGPVGLDAGYFHRDATNLIDFVRTPGDPVFHARNV
                     LSAVTDGIEVSAAWESGRPAFLSTLSVQAAWIFTDLAALSASAGGAAEGRYVLDPLHV
                     KADACVGVALPAALGLTARLTYFSRPSFADGVWLLSSRLSWQAFQGRILELFVEGENL
                     GNVRYEDVPGVPLPGRTALAGFNLTW"
     gene            1851..2192
                     /locus_tag="PLB01_17405"
     CDS             1851..2192
                     /locus_tag="PLB01_17405"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQQ79129.1"
                     /translation="MDDVPLKTRAPRVLLDAEVTYWRDGNHAAGRLTNLSESGFFTAT
                     DEPAPVGSRLEVSFTLPHDRSGKIVTGEVIVVRRAEEGRPGFGARFFRLPQSAARMIE
                     EFLARSARRRN"
     gene            complement(2193..2645)
                     /locus_tag="PLB01_17410"
     CDS             complement(2193..2645)
                     /locus_tag="PLB01_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HQQ79130.1"
                     /translation="MAPRVDWHRYFMNIAHEVASRATCPRKHVGAVVVREKRILSTGY
                     NGSLRGLPHCDEAGCLMEDGHCVATVHAEANAILQAAMNGVRIEGGEIYTTASPCWNC
                     FKLIANSGLSAVYFGEFYREKRIHDYAEKVGIELVDLSALATLSPPPA"
     gene            complement(2680..>4608)
                     /locus_tag="PLB01_17415"
     CDS             complement(2680..>4608)
                     /locus_tag="PLB01_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79131.1"
                     /translation="GFVLVEPLGEVAAVRPVITFRPAASDFTLIQMTLDGVIVTQLVS
                     ANGGIWRFEPFVEISGGLHTVDVVYTSHGVSSAVAWTFQTSAPPPHPESAFGWSFGAE
                     VARTHGEPVGREGPTTNVTSITGVPHLEGSVTDNVAGVQTAFNGTLAQNVDPNALPAH
                     VTPPAVVVTAKAGVVSGAIGNGPAETFAPSPLLQTVSTRRGVELGLDTKLVSMRAFGN
                     IDDGLPASTGVNEFRQTLYGVAVMPKLGTDRVKFRVLYQYVEDEKDPLFVAPPALSPP
                     YGEAGAAAPAQTQPAFYGSAPKKGHLLSFSADLLLEPTSNLMLRGEAVRSEFTTDKTS
                     RALQDDWAYAILLTANPLGFSVAAGVRLVGDQFGAPANPALIAGRKVYDGSLSRSFGA
                     LSLSVNYAHTGDSGGAGAGTTGFTTPSGRADAGALTASYSFAASRTSVSASLQENKAE
                     SSGSTNKSTNLNLSVAQPLGEFQFSLGLLGGRQETDGLYLSEAETRGVTLGLSRQGNV
                     FSVQTSAGVNQSRNRLTGDLTTSVNAALTPDVALFNRMVNITPLGSWTRQTATGGLSD
                     SSSWSWGGRLTVRTWGKIRGFAVWAQYLESAMAPHAEDQPITRDRRFGAGLAVLFGGG
                     SLAPSVAQQTITPQLGLR"
BASE COUNT          639 a         1633 c         1655 g          681 t
ORIGIN      
        1 tccggcggga ggccagcgtc gtcgcgagcc ggctcgacga cgcgcctccg accgccgtcg
       61 tccgcgtcgt gtcccgcgac gagatcgacg cgctgccggg cgcgcacacc gttccggacg
      121 tcctgcgctc cgtgctcggc gtcgacgtga ggcggcgcgg cccgggcggc gtgcaggcgg
      181 acgtcgggat ccgcggcggg gacttcaacg ggacgctcgt gctcgtggac ggccagcccg
      241 tgaacgaccc gcagagcaac cacctgtcct tcgacctcga cgttccggtc gcggcgatcg
      301 agcgcatcga ggtcctcgcg ggccccgggt cgtccgtctg gggctcgaat cccgtcggcg
      361 gcgccgtgaa catcgtcacg cgcggcggcg ccctcgggcg cgcagcggtc caactcgagg
      421 ggcgctacgc tcacgggacg cagtccctcg accagggagg cgcacgcctc gcggtgcgcg
      481 tggccgagcc ggtcacggtc ggcgtcgact ggtggcgcgc ggagaccgcg ggtttccggg
      541 acgacaccga ggccgcgacg gacatcctgc gcctctcgag ccgctgggaa accgggaagg
      601 gtcccgtgac gctcggcctc gggatcgcgg agcgccggtt cggcgcgtac gcgttctacg
      661 gaacggcgtt cccgaaccag caggagtcga cgcggacgcg cacggcgacg ctcggcggca
      721 ctctcgcgtt cggcggcttc acgctgacgc cctccgtctt cgtccgcgcc caccacgacg
      781 acttcgtact cgaccgcggg aaccccgcgt tctacgagaa cctgagcgac accgtgacct
      841 cgacggcccg gctgtcggtg agccgtgccg tgctcggcgg ctccgtcgcg gccggggtgg
      901 agggcgggcg cgacaccatc gcctcgtcga acctcggcga acacgcccgg gaccgcgggg
      961 cgatcttcgc cgagttcgca cgttcgtgga gcgtcgccga tcccggcttc ggcggcgtcc
     1021 gcgtcggact gagggcggat acgtacgacg aatacggctc ccgcgtctcg ccctacgcgg
     1081 gcctgaccgt ccgcgccgcg ccgccgctca cgctccgcgc ctccttcggg acgagcttcc
     1141 gcgtcccgac gttcacggag ctctactaca gggatccgca gtccgccggg aacccggggc
     1201 tcgcacccga aaccgcgtgg tcggtcgacg cgggcgtgaa gctcgcggca gggcctgtcg
     1261 gcctcgacgc cgggtacttt caccgggacg cgacgaacct catcgacttc gtgcgcacgc
     1321 ccggcgaccc cgtgttccac gcccggaacg tcctctccgc cgtcacggac gggatcgagg
     1381 tctccgcggc gtgggagagc gggcgccccg ccttcctgtc cacgctgtcc gttcaggcgg
     1441 cctggatctt cacggacctc gcggctctct ccgcctccgc aggcggcgcg gcagaagggc
     1501 gctacgttct cgaccccctc cacgtgaaag cggacgcctg cgtcggcgtc gcgctccccg
     1561 ccgccctcgg gctcaccgcg cgcctcacgt acttctcgcg cccgagcttc gcggacggcg
     1621 tctggctgct gtcctcccgt ctctcgtggc aggccttcca gggccgcatc ctcgagctct
     1681 tcgtcgaggg agaaaacctc gggaacgtcc gctacgagga cgtgccgggc gtccccctgc
     1741 ccgggcggac ggccctcgcg ggcttcaacc tgacctggtg acggcgcgca cagccgcgga
     1801 cggtttcggc gcgtaggctc gtggagccac gcgacacttc atcggaacac atggatgacg
     1861 tccctctgaa gacccgcgcc ccccgggttc tgctcgatgc cgaggtcacg tactggcgcg
     1921 acgggaatca cgccgcgggc cggctgacca acctctccga gagcgggttc ttcacggcca
     1981 ccgacgagcc cgcgcccgtc ggctcgcgcc tcgaagtcag cttcacgctc ccccacgacc
     2041 ggagcgggaa gatcgtgacg ggcgaggtca tcgtggtgcg acgcgccgag gaaggccgcc
     2101 cgggtttcgg ggcgcgcttc ttccgccttc cgcagtccgc cgcccggatg atcgaggaat
     2161 tcctcgcgcg ctccgcgcgc cgccgcaact gatcaggcgg gcggcggcga gagggtcgcg
     2221 agagccgaga ggtccacgag ctcgatcccg accttctccg cgtaatcgtg gatgcgtttt
     2281 tcgcggtaga actcgccgaa atacacggcc gaaaggcccg agttcgcgat gagcttgaag
     2341 cagttccagc agggcgacgc cgtcgtgtag atctccccgc cctcgatcct cacgccgttc
     2401 atcgcggcct gcaggatcgc gttggcttcc gcgtgaacgg tcgcgacgca gtgtccgtcc
     2461 tccatcaggc agccggcctc gtcgcagtgc ggaaggcctc ggagcgagcc gttgtacccc
     2521 gtcgagagga tgcgcttctc gcgtacgacg accgcgccga cgtgcttgcg cgggcacgtc
     2581 gcccggctcg cgacctcgtg ggcgatgttc atgaagtagc ggtgccagtc gacccggggc
     2641 gccatgggac cgggatcata accgggcgcc tccgggtcgt tacctcaggc cgagctgcgg
     2701 cgtgatcgtc tgctgggcga ccgacggcgc aagcgagccg ccgccgaaga gaaccgcgag
     2761 acccgctccg aatcgccggt cgcgcgtgat cggctggtcc tcggcgtgag gcgccatcgc
     2821 ggactcgagg tactgcgccc acacggcgaa gccccggatc ttcccccacg tgcggaccgt
     2881 gagccgtccg ccccagctcc acgagctcga gtccgagagg ccgcccgtcg ccgtctggcg
     2941 cgtccacgaa ccgaggggcg tgatgttcac catgcggttg aacaacgcga cgtcgggcgt
     3001 gagggcggcg ttgacgctcg tcgtgaggtc gcccgtgagg cggtttcgcg actggttgac
     3061 gccggcgctc gtctggacgg agaagacgtt cccctggcgc gagaggccca gggtcacgcc
     3121 gcgggtctcg gcctcgctca ggtagaggcc gtccgtctcc tggcggcctc cgagaaggcc
     3181 gagggagaac tggaactctc cgaggggctg ggcgaccgac aggttcaggt tcgtggactt
     3241 gttcgtcgac ccggaggact ccgccttgtt ctcctgcagc gaggcggaaa cggacgtgcg
     3301 gctcgcggcg aagctgtagg acgccgtgag ggctcccgcg tcggcccggc cgctcggcgt
     3361 cgtgaagccc gtcgtcccgg ccccggcgcc gcccgagtcg cccgtgtgcg cgtagttcac
     3421 cgagagcgag agcgcgccga acgatcggga gagcgagccg tcgtagacct tgcggcccgc
     3481 gatgagcgcc ggattcgccg gcgcgccgaa ctggtcgccg acgaggcgta cgcccgccgc
     3541 gaccgagaac ccgagcgggt tcgccgtgag gaggatcgcg taggcccagt cgtcctgcaa
     3601 cgcccgggac gtcttgtcgg tcgtgaattc gctgcgcacg gcctcgcccc gcagcatgag
     3661 gttcgacgtc ggctcgagga gcaggtcggc ggagaacgag aggaggtgcc ctttcttcgg
     3721 cgccgagccg tagaacgcgg gctgcgtctg cgccggcgct gccgcgcccg cttccccgta
     3781 gggcggggac agcgcgggcg gggcgacgaa gagcgggtcc ttctcgtcct cgacgtactg
     3841 gtagaggacg cggaacttga cgcgatccgt tccgagtttc ggcatgaccg cgacgccgta
     3901 gagcgtctgg cggaactcgt tgactccggt cgaggccgga aggccgtcgt cgatgttgcc
     3961 gaatgcgcgc atcgagacga gcttcgtgtc gagaccgagc tcgacgccgc ggcgcgtcga
     4021 gacggtctgg aggagagggg acggtgcgaa cgtctcggcc gggccgttgc cgatcgcccc
     4081 cgacacgacg ccggcctttg cggtcacgac gacggcgggc ggcgtcacgt gcgccggcag
     4141 ggcgttcggg tcgacgttct gggcgagcgt cccgttgaac gccgtctgga cgcccgcgac
     4201 gttgtcggtc acggagccct cgaggtgcgg gacgccggtg atcgaggtca cgttcgtcgt
     4261 cgggccctcg cggccgacgg gttcgccgtg cgtgcgcgcg acctcggctc cgaacgacca
     4321 gccgaaggcg gactccgggt gcggcggcgg cgcggacgtc tggaacgtcc acgcgaccgc
     4381 cgaggagact ccgtgagacg tgtagacgac gtccacggtg tgcagcccgc ccgagatctc
     4441 gacgaagggc tcgaagcgcc agatgccccc gttcgccgag acgagctgag tcacgatgac
     4501 gccgtccagc gtcatctgga tgagcgtgaa gtcggacgcc gcgggccgga acgtgatgac
     4561 gggccggacg gccgcgacct cgccgagcgg ctcgacgagg acgaagcc
//
LOCUS       DAOMFY010000307         4581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6797_length_4581_cov_6.183385, whole genome shotgun sequence.
ACCESSION   DAOMFY010000307 DAOMFY010000000
VERSION     DAOMFY010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_4581_cov_6.183385"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLB01_17420"
     CDS             complement(<1..669)
                     /locus_tag="PLB01_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79132.1"
                     /translation="MPAEDVEAALRDEGLAGFLPASRARIAALALVAPAVHGRPVPSV
                     PALREPADYAAPGAPVLRTFLLQGIVLAPVEALEALLALPAVLRVRPLSLTAGDDLEY
                     WSEVARWTYDLLLRRRIAPTADQGRPRWRPVLTDPHEKERFRRFAAALPPVSRALSEE
                     GAAPPAEGGLPPAAAVLQGFLDAVGSFAIAAQVLYSIGLVLGGWYLAPGLAREQRSVV
                     SLGAC"
     gene            852..1322
                     /locus_tag="PLB01_17425"
     CDS             852..1322
                     /locus_tag="PLB01_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79133.1"
                     /translation="MSAFVETACEDDELPEKVAGAVLTYQQIPLKRFSKLPEDTRRAY
                     VRRTLRDKRAEDLALYVLSAALTRRQAELISAFLDAVKLPHEGASLTTEGEIPQPSAA
                     VLAKGVDAVLAKWPARDVSLYLHAFAAQPDVHWPLLDGMLVADGRLSLEDRSAQ"
     gene            1450..2856
                     /locus_tag="PLB01_17430"
     CDS             1450..2856
                     /locus_tag="PLB01_17430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ79134.1"
                     /translation="MADGISYAFQGKIFAAGRFWLEPPLVPEAFKCQNVLLDAAHWCG
                     IYPPGFPLLLAVGWLLGAPSLVNPFLLGLAVLGVFRLGAVLYDDVTGLLGALLLAVSP
                     FALLMGAGFMGHVASLSVFTWGLVFLAQALQGAAVRPLLVSGFLGAFGVLVRPQAAVF
                     FLAPALVGVLVARRKELARSVGLLVLGGAPPLAFLLAYNFALSGHPLRMAYLVWDPNL
                     SFTYGLPVLQLFSSHFPWFLHDLGATLWGFPWGDLVPLLVLVIPAPGGRRDAWLAACV
                     LALLVGFSAYRFYEINHSGPRYAFEALGALALLSSRALLRIGTFAGRLLARVRLGFLR
                     PGAAAAAVGFLAVFPLVTLLPRQAEALSHAYHAHTGEPLRRLAAAGVGPSALVLVAGN
                     TVEWTYGSFLLENGLDPRRAPRVFARDLPEKRAELLAAYPRREVWRVTVTLAPLPHPN
                     AWIDNTWDVVDVVWTRLR"
     gene            complement(2853..3524)
                     /gene="rsmI"
                     /locus_tag="PLB01_17435"
     CDS             complement(2853..3524)
                     /gene="rsmI"
                     /locus_tag="PLB01_17435"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQQ79135.1"
                     /translation="MGRLLVVATPIGNLDDLSPRAKDALANASVVYCEDTRRTGNLFA
                     RHGLTTPRVSCHGHNEHARVDEALRRLAAGETLALVSDAGTPLVSDPGERLVAAAAAA
                     GHRVEAIPGPSAVLAILSVSGFPAVPFTFLGFPPSRRGERDRWYAAHAGRAGTVVLFE
                     SPFRVAESLEALAKAWGDPPVALGRELTKLHEEVLRGKASEVARALAARPGVKGEIVI
                     AAAAS"
     gene            3575..4243
                     /locus_tag="PLB01_17440"
     CDS             3575..4243
                     /locus_tag="PLB01_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79136.1"
                     /translation="MFGVLAAWLLATQAPAPPAPSPAPLPGTVVLDRIAAVVGDDLIL
                     ESELRKLAGVGYLPREAGESDAAYRDRVLSVRVVEVLRDRQLRRTGGIEPKRGEVEAR
                     VAALEDRVVKERGEPLDAILARAGATREELFLFVKRGMALESFVRERLSPGIKTTETE
                     LRAYYDGPFREEARAKGLASLPPYEDVSEQIRELVRERKLNAEIERWTEQLRAETRVL
                     IYRR"
     gene            complement(4227..>4581)
                     /locus_tag="PLB01_17445"
     CDS             complement(4227..>4581)
                     /locus_tag="PLB01_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQQ79137.1"
                     /translation="DGRLTTLLRLHGDAARELNESPPAYADLYDPARSLRLGARTLKN
                     LLVRFDGDDALAASGYNAGAGQTALWAGGARRPVEALLASISYPETRTYVRRVLSNRI
                     LYRMSGPRPPVSGGK"
BASE COUNT          567 a         1662 c         1680 g          672 t
ORIGIN      
        1 gcaggccccc aggctcacga cgctgcgctg ctcgcgcgcg aggccagggg cgaggtacca
       61 gccgcccagc acgaggccga tcgaataaag cacctgggcg gcgatggcga agctgccgac
      121 ggcgtccagg aagccctgca ggacggccgc cgcgggcggg aggcctcctt cggccggcgg
      181 cgcggcgcct tcctccgaga gcgcgcgcga cacgggcggg agcgcggcgg cgaagcgccg
      241 gaagcgttcc ttctcgtggg gatccgtcag gaccggccgc cagcgcggcc ggccctggtc
      301 ggcggtgggc gcgatgcggc ggcgcaggag gaggtcgtac gtccagcgcg cgacctccga
      361 ccagtactcg aggtcgtcgc cggccgtgag ggacagcggc ctcacgcgca ggacggcggg
      421 cagcgcaagg agcgcctcga gcgcctcgac gggcgcgaga acgatgccct ggagcaggaa
      481 cgtccggagg acgggcgcgc ccggagcggc gtagtccgcg ggctcgcgca gggccgggac
      541 ggacggcacg ggccttccgt gcacggccgg ggccacgagg gcgagggctg cgatccgggc
      601 gcgggacgcc gggaggaagc cggccaggcc ctcgtcgcgc agggccgcct ccacgtcttc
      661 cgccggcacg gcgcgcggat gcgcgtcgcg cgcgtctcgg gcgacgtagg aaccttcggc
      721 ccagaacgcg aagccggaag ggagcgggga gccgtgcagg acgatcagag ccgtccattg
      781 tcgggtatcc tccgcccccg atgaaagccc caagatcgca cgagcttttc cggcttcttt
      841 cggcggccga ggtgtccgcc ttcgtcgaaa cggcgtgcga ggacgacgag ctgcccgaaa
      901 aggtcgccgg cgccgttctc acctaccagc agatccccct gaagcgcttc tcgaagctgc
      961 cggaagacac gcggcgggcc tacgtgcgac ggacgctccg cgacaagcgg gccgaggacc
     1021 tcgcgctcta cgtcctttcg gcggcgctca cgcgccggca ggcggagctc atttcggcgt
     1081 tcctcgacgc cgtgaagctc ccgcacgagg gggcgagcct gacgaccgag ggggagatcc
     1141 cacaaccgtc cgcggcggtc ctcgcaaagg gcgtcgacgc ggtgctcgcg aaatggcccg
     1201 cgcgcgacgt gtcgctctac cttcacgcgt tcgcggcgca gcccgacgtg cactggccgc
     1261 tcctcgacgg gatgctcgtc gccgacggcc gcctgtcgct cgaagaccga tcggcccagt
     1321 agcgctgcgg tgagcgaggc gccgagtctc cggatgcccc ggccggccgc tctcgccgca
     1381 ggtctcgccg tctctgccgc cgtcgcgagt gcggtactgg ggcagcgcgt attcggcggc
     1441 gtgccgcacg tggccgacgg aatctcgtac gctttccagg ggaagatctt cgcggcgggc
     1501 cgtttctggc tcgagccgcc gctcgtgccg gaggcgttca agtgccagaa cgtccttctc
     1561 gacgcggctc actggtgcgg catctatcct ccggggtttc ctctgctcct cgccgtgggc
     1621 tggctcctcg gggccccctc cctggtcaac ccgttcctgc tcgggctggc cgtcctgggc
     1681 gtcttccgcc tcggagcggt cctctacgac gacgtgacgg gtctcctcgg cgcgctcctc
     1741 ctggcggtct cgccgttcgc gctgctcatg ggtgcaggct tcatggggca cgtggcaagc
     1801 ctcagcgtct tcacgtgggg cctcgtcttc ctcgcgcagg ccctccaggg agcggccgtg
     1861 cgcccgctcc tcgtgtccgg tttcctcgga gctttcggag tcctcgtgcg cccccaggcg
     1921 gccgtcttct tcctggcacc ggcgctggtc ggcgttctcg tggcccgcag gaaggagctc
     1981 gcgcggtcgg tcggactgct cgtgctcggc ggagcgccgc ctctcgcctt ccttctcgcc
     2041 tacaacttcg ccctgtcggg ccatccgctg cgcatggcgt atctcgtctg ggacccgaac
     2101 ctgtcgttca cgtacggcct tccggtcctg cagctctttt cgtcccactt tccgtggttc
     2161 ctgcacgacc tcggcgcgac gctgtggggg tttccgtggg gagacctcgt gccccttctc
     2221 gtgctcgtga tcccggcgcc cggcggccga cgggacgcct ggctggccgc gtgcgtcctc
     2281 gcgctcctcg tcgggttcag cgcctaccgc ttctacgaga tcaaccactc cggcccccgt
     2341 tacgcgttcg aggcgctcgg ggcgctcgca ctcctctcct cgcgcgcgct tctccggatc
     2401 ggaaccttcg cgggacgcct cctcgcccgc gttcgcctcg gcttccttcg cccgggagcg
     2461 gcggcggccg cggtcggctt cctcgccgtc ttcccgctcg tgacgctcct gccgcgacag
     2521 gccgaggcgc tctcccacgc ctaccacgcg cacaccgggg agccgctgcg ccgcctcgcg
     2581 gccgccggag tcggcccgtc ggcgctcgtg ctcgtcgcgg ggaacaccgt ggagtggacg
     2641 tacggctcgt tcctgctcga gaacggtctc gacccgcggc gcgccccccg agtcttcgcg
     2701 cgcgacctcc cggagaaacg cgccgaactc ctcgccgcct atccgcgacg cgaggtgtgg
     2761 cgggtcaccg tgacgctcgc gcccctgccg cacccgaacg cgtggatcga caacacgtgg
     2821 gacgtcgtgg acgtcgtctg gacccggctc cgctaggagg cggcggccgc gatcacgatc
     2881 tcgcccttca cgcccggccg cgccgcgaga gcccgcgcga cctcggaggc cttcccgcgg
     2941 aggacttctt cgtggagctt cgtgagttcg cggccgaggg cgacgggcgg atcgccccaa
     3001 gccttcgcga gcgcctccag agactcggcg acgcggaacg gcgactcgaa gaggacgacc
     3061 gtgcccgcgc gccccgcgtg ggcggcatac cagcggtcgc gctcgccgcg gcgcgaaggc
     3121 gggaagccga ggaacgtgaa cggcacggcc gggaagcccg acacggagag gatcgcgagg
     3181 acggccgacg gaccgggaat cgcctccacg cggtgcccgg ccgcggccgc ggccgccacg
     3241 aggcgctcgc ccgggtccga gacgagcggc gtccccgcgt ccgagacgag tgcgagcgtc
     3301 tcgcccgcgg cgaggcgccg gagcgcctcg tcgacgcgcg cgtgttcgtt gtggccgtgg
     3361 caggagacgc gcggcgtggt caggccgtgg cgcgcgaaga ggtttccggt gcggcgcgtg
     3421 tcctcgcagt agacgacgga ggcgttcgcg agcgcgtcct tcgcgcgcgg cgagaggtcg
     3481 tcgagattcc cgatcggcgt cgcgacgacg aggaggcggc ccatgcgccc attgtcgcag
     3541 cgccggccct gcggcgcctg ctagcatcgc ccgcgtgttc ggcgtcctcg cggcgtggct
     3601 cctcgcgact caggcccccg cgccgcccgc gccctcgccg gcgcctcttc cgggcacggt
     3661 cgtcctcgac cggatcgcgg ctgtcgtcgg cgacgacctg attctcgaga gcgaacttcg
     3721 gaagctggcg ggcgtcgggt acctgccgcg cgaggcgggg gagtcggacg ccgcgtaccg
     3781 agaccgcgtt ctctcggttc gcgtggtgga ggtcctgcgc gaccggcagc ttcgcaggac
     3841 gggcggaatc gagcccaagc gcggggaggt cgaggcccgc gtcgccgccc tggaagaccg
     3901 cgtcgtgaaa gagcgcggcg agccgctcga cgcgatcctc gcgcgcgccg gggcgacgcg
     3961 tgaggagctc ttcctcttcg tgaagagggg catggcgctc gagagcttcg tgcgggagcg
     4021 cctctcgccg gggatcaaga cgacggagac ggagctgcgc gcgtattacg acggcccgtt
     4081 ccgggaggag gcgcgtgcga agggcctcgc gtcgctgccg ccctacgagg acgtttcgga
     4141 gcagatccgc gagctcgtgc gcgaacgcaa gctcaacgcc gagatcgagc gctggaccga
     4201 gcagctgcgc gccgagacgc gcgtcctcat ttaccgccgc tgacgggggg gcgcggcccg
     4261 gacatccggt agaggatcct gttcgagaga acgcgccgga cgtacgtgcg cgtctccggg
     4321 tagctgatcg acgcgaggag cgcctccacc gggcgccgcg cgccgcccgc ccagagcgcc
     4381 gtctggcccg cgcccgcgtt gtagcccgag gccgcgagcg cgtcgtcgcc gtcgaagcgc
     4441 acgaggaggt tcttgagcgt gcgcgcgccg aggcgcaggg agcgcgccgg gtcgtacagg
     4501 tccgcgtacg cgggagggct ctcgttcagc tcgcgtgccg cgtcgccgtg caggcgtagg
     4561 agggtcgtga gccttccgtc g
//
LOCUS       DAOMFY010000308         4571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6824_length_4571_cov_5.233835, whole genome shotgun sequence.
ACCESSION   DAOMFY010000308 DAOMFY010000000
VERSION     DAOMFY010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6824_length_4571_cov_5.233835"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLB01_17450"
     CDS             complement(<1..223)
                     /locus_tag="PLB01_17450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ79138.1"
                     /translation="MSRAALVALLAATATASYLARVNVSVAGALMMKELALSQIEMGR
                     VFSAFLLGYALCQVPAGMLADRFGARRVLG"
     gene            complement(220..672)
                     /locus_tag="PLB01_17455"
     CDS             complement(220..672)
                     /locus_tag="PLB01_17455"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ79139.1"
                     /translation="MAGPTIGDATHEERLWAGRLMASSEPWITLGRGEDACRAAALDP
                     AYVTLVARGDAAPLGFARFHPRGVAGSPYLASIAVAPEARGLGVGSALLAAGEARFAG
                     ARWMFLCVSDFNPRARHLYERSGYRFVGALPDYVVDGFAEHLMVKRLG"
     gene            699..1385
                     /locus_tag="PLB01_17460"
     CDS             699..1385
                     /locus_tag="PLB01_17460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQQ79140.1"
                     /translation="MTSVVLVRHGQASFGAADYDVLSETGRRQARVAGEALRGRIGDV
                     RAVVTGAMRRQRDTAAACLDAMGLSPAWTEDAAWNEFDHEEVLRVYEPRWADHAQLHR
                     DLEAAAFAGAFERAFHAAVARWTGGAHDAEYRETWPGFLARVDEGLARLSRAPGGTVL
                     VFTSGGPISAACRAPLGAADAAAVFAHAFRLANASVTTVRLGPAGLELAAFNDHAHLA
                     GCEEGLVTFR"
     gene            complement(1399..>1773)
                     /locus_tag="PLB01_17465"
     CDS             complement(1399..>1773)
                     /locus_tag="PLB01_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79141.1"
                     /translation="STAPLDGEGRSADWYVKWSSKKAGKVNMMSVKNGVLSTFEVPTA
                     GGRVIVVAAQSIFDSKKLLAMADAAGGAAHRAKGAAVSLGLVQNPVVNGPLWHVSYGR
                     DGKEVFHVGIEANGGKTTVLSN"
     assembly_gap    1774..1873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1874..2139
                     /locus_tag="PLB01_17470"
     CDS             <1874..2139
                     /locus_tag="PLB01_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79142.1"
                     /translation="HLTYQSAERPSPSSGAVESVPKSNISASGCHFAAAAFARPYAVW
                     ASTGPAARAAGAAARARHTASARVEAVFVKGSSCWPRIVPLPS"
     gene            2150..3370
                     /locus_tag="PLB01_17475"
     CDS             2150..3370
                     /locus_tag="PLB01_17475"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQQ79143.1"
                     /translation="MKTRLAGALALLALGLLGAPAKADGGDASAPIFTALPPAPSEHV
                     TDNAQFLSVEARARLEAKLKQFRSDHAFVRLYVYTLRSANGSAAAAAQELYRRWKMRD
                     RELYDGLATIFVFWNEKKALVMLGQGAPARTEEALADLSPDLAAIFGADPEGAISRVI
                     DRIGASLAGDTASWIDAPDVPLDPGGPLYGNPDFADEAETRALEAAVRRASTPDHPIV
                     LVLNPAKGLDSPQRRAERLEKAWPGRILLVAFRSDFAASLAVPDALRDRFPDEQRQRL
                     LREVRAAMTNATYSRTLARAVGEIGMLADGKTPPPWVAWKHPLKTIGGGQDEDPAPLA
                     LGIGIGAVLLFLVSFFLYMLVTNPKGVLLWIGVNLAEAVIGGLFSGGGSSSGGGGFSG
                     GGGSFGGGGASGSW"
     gene            3373..3828
                     /locus_tag="PLB01_17480"
     CDS             3373..3828
                     /locus_tag="PLB01_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQQ79144.1"
                     /translation="MAIVLRRHQKDFLSRLDVAALEAAIREAERATTGEIRVAVLPRV
                     RGGLDNAVKPAAERLGMTRTKERNGVLILVDPARRAFLVWGDTAIHERVGEAFWKEIA
                     AGIEERFRAGDFTGGLVRGIETAGRALAAHFPPGPGVNPNELPDRVDVS"
     gene            3858..4364
                     /locus_tag="PLB01_17485"
     CDS             3858..4364
                     /locus_tag="PLB01_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79145.1"
                     /translation="MRRIAAIALPLLLLSSRPAPAAQPLPDGFHGLKWRSAVAPGMKL
                     KQDVSTDDNAAYTRPNDVKKLYGAALESVTYGYCKGQFCSATVQTDAGQGAKLLKAFT
                     AEWGEPEKVAANVFVWKDATETMVIFRGTDLEGSASRAEVYVVCKPLVEAANKAERER
                     REKLLGIK"
     gene            complement(4357..>4571)
                     /locus_tag="PLB01_17490"
     CDS             complement(4357..>4571)
                     /locus_tag="PLB01_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HQQ79146.1"
                     /translation="GARAALVTATAGGQHAFALGAGEGFGDVQALGREVAALLGGKGG
                     GSGRVFQGKAPALDGADRAAARLAAT"
BASE COUNT          625 a         1673 c         1586 g          587 t
ORIGIN      
        1 tcccgaggac gcggcgggcg ccgaagcggt ccgcgagcat cccggccggc acctggcaga
       61 gcgcgtaccc gaggaggaac gcgctgaaga cgcgccccat ctcgatctgg gagagagcga
      121 gctccttcat catgagcgcg cccgcgaccg acacgttcac gcgcgcgagg tagctcgccg
      181 tcgcggtggc cgcgaggagg gcgacgagcg cggcgcggct cagccgagcc tcttgaccat
      241 caggtgctcg gcgaacccgt ccacgacgta atcgggcagc gcgccgacga aacggtagcc
      301 gctgcgctcg tagaggtggc gcgcccgagg gttgaaatcc gagacacaca ggaacatcca
      361 gcgcgcgccg gcgaaccgcg cctcccccgc cgcgagaagc gccgagccga cgccgaggcc
      421 gcgcgcctcc ggagcgaccg cgatcgacgc gaggtacgga gaaccggcga ccccgcgcgg
      481 gtggaagcgg gcgaagccga gcggcgcggc gtccccgcgc gcgacgagcg tcacgtacgc
      541 ggggtcgagc gcggcggcgc ggcacgcgtc ctcgccgcgg ccgagcgtga tccacggctc
      601 gctcgaggcc atgagacggc ccgcccacag gcgttcctcg tgagtcgcgt ctccgatcgt
      661 cgggccggcc atgccgctcc gaggataatc ccgcgcgcat gacctccgtc gttctcgttc
      721 gccacggcca ggcctccttc ggcgccgccg actacgacgt cctctcggag accgggcggc
      781 ggcaggcccg ggtcgccggc gaggcgctcc gcggacggat cggggacgtg cgcgcggtcg
      841 tcacgggcgc catgcgccgg cagcgcgaca ccgccgcggc gtgcctcgac gcgatgggtc
      901 tctccccggc gtggacggag gacgcggcgt ggaacgagtt cgaccacgag gaggtcctgc
      961 gcgtctacga gccgcgctgg gcggaccacg cgcagctcca ccgcgacctc gaggccgcgg
     1021 cgttcgcggg ggccttcgag cgcgccttcc acgccgccgt agcccgctgg acaggcggcg
     1081 ctcacgacgc ggagtaccgc gagacgtggc cgggcttcct cgcgcgcgtg gacgaagggc
     1141 tcgcgcggct ttcgcgcgcc ccgggcggga ccgttctcgt cttcacgtcg ggcggcccga
     1201 tctcggccgc gtgccgcgcg cccctcggcg cggcggacgc cgcggccgtc tttgcgcacg
     1261 cgttccggct cgcgaacgcg agcgtcacga ccgtgaggct cgggccggcc ggcctcgagc
     1321 ttgcggcatt caacgaccac gcccacctcg ccggctgcga ggagggcctc gtcacgttcc
     1381 gctgaggccg gccgcgggtc agttcgagag gacggtcgtc ttgccgccgt tcgcctcgat
     1441 gccgacgtgg aagacttcct tgccgtccct gccgtagctc acgtgccaca acggaccgtt
     1501 cacgacgggg ttctgtacga gaccgaggga cacggcagcg cccttggccc ggtgcgcggc
     1561 gccgcccgcg gcgtccgcca tcgcgaggag cttcttcgag tcgaagatcg actgagccgc
     1621 cacgacgatc acgcggccgc ctgccgtcgg aacctcgaac gtcgagagca cgccgttctt
     1681 aaccgacatc atgttcacct tgccggcctt cttcgacgac cacttgacgt accagtccgc
     1741 cgagcggccc tcgccgtcga gcggcgccgt cgannnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnaccactt gacgtaccag tccgccgagc ggccctcgcc gtcgagcggc
     1921 gccgtcgaga gcgtgccgaa gtcgaacatc tcggcgtcgg gctgccactt cgcggcggcg
     1981 gccttcgcga ggccctacgc ggtctgggcg tccacggggc cggcggcgcg cgcggcgggc
     2041 gcggcggcca gggcgaggca cacggcgagc gcgagggtcg aggctgtctt cgtcaagggt
     2101 tcctcctgct ggccgcgaat cgtacccctg ccgtcctaag atgcacgccg tgaagacacg
     2161 gctcgcggga gctcttgcgc tgctggccct cggcctgctc ggggcgccgg ccaaggccga
     2221 cggcggcgac gcgtccgctc cgatcttcac cgcgcttccc ccggcgccct ccgagcacgt
     2281 gacggacaac gcgcagttcc tctcggtcga ggcgcgcgcg cgcctcgagg cgaagctcaa
     2341 gcagttccgg agcgaccacg cgttcgtccg cctctacgtg tacacgctgc gttcggcgaa
     2401 cggctccgcc gccgcggcgg cccaggagct ctaccgccgt tggaagatgc gcgaccgcga
     2461 gctctacgac ggcctcgcga cgatcttcgt cttctggaac gagaagaagg cgctcgtcat
     2521 gctcggtcag ggcgcgcccg cgcgcaccga ggaggcgctc gccgacctct cccccgacct
     2581 cgcggcgatc ttcggcgccg accccgaggg cgcgatctcg cgcgtgatcg accgcatcgg
     2641 cgcgagcctc gcgggcgaca cggcctcctg gatcgacgct cccgacgtgc ccctcgaccc
     2701 cggcgggccg ctctacggca acccggactt cgcggacgag gccgagacgc gcgcgctcga
     2761 ggcggccgtc cggcgcgcgt ccactcccga ccatccgatc gtcctcgtcc tgaatcccgc
     2821 caagggcctg gactccccgc agcggcgcgc cgagcggctc gagaaggcct ggccgggccg
     2881 gatcctcctc gtcgccttcc ggtccgactt cgccgcgtcg ctcgccgttc cggacgcgct
     2941 gagggaccgt tttcccgacg agcagcgcca gcgcctgctc cgcgaggtcc gggcggcgat
     3001 gacgaacgcg acgtactcgc ggacgctcgc gcgcgcggtc ggcgagatcg ggatgctcgc
     3061 cgacgggaag acgccgccgc cgtgggtcgc gtggaagcac ccgctcaaga cgatcggcgg
     3121 cgggcaggac gaagacccgg cgcctttggc gctcggcatc gggatcggag ccgttcttct
     3181 cttcctcgtc tcgttcttcc tctacatgct cgtcacgaac ccgaaggggg tcctcctctg
     3241 gatcggcgtg aatctcgccg aggcggtcat cggcggtctc ttcagcggcg gaggcagcag
     3301 cagcggcgga ggcgggttct cgggaggcgg aggaagcttc gggggcggcg gcgcctcggg
     3361 gagctggtga acatggccat cgtcttgcgc aggcatcaga aggacttcct ctcgaggctc
     3421 gacgtcgcgg ccctcgaggc cgcgatccgc gaggccgagc gcgcgacgac gggcgagatc
     3481 cgcgtcgcgg tcctgccgcg cgtccggggc gggctcgaca acgccgtgaa gcccgcagcc
     3541 gagcgcctcg gcatgacgcg tacgaaagag cgcaacggcg tcctcatcct cgtcgatccc
     3601 gcgcggcgcg cgttcctcgt ctggggcgac acggcgatcc acgagcgcgt gggcgaggcg
     3661 ttctggaagg agatcgccgc cggaatcgag gaacgattcc gggccggcga cttcaccggc
     3721 ggcctcgtgc gcgggatcga gacggccggc cgcgcgctcg ccgcgcactt cccgccgggg
     3781 ccgggcgtca acccgaacga gctgcccgac cgcgtcgacg tctcctgagc gaagatggag
     3841 cggccgaagg agaccccgtg agacgtatcg ccgccatcgc gctccccctc ctcctcctct
     3901 cctcccgtcc cgctcccgcc gcgcagccgc tgcccgacgg gttccacggg ctgaagtggc
     3961 gctccgccgt cgccccggga atgaagctca agcaggacgt ctcgaccgac gacaacgcgg
     4021 cctacacgcg cccgaacgac gtcaagaagc tgtacggcgc cgccctcgag agcgtcacgt
     4081 acggctactg caagggccag ttctgctccg cgaccgtcca gaccgacgcc ggccagggcg
     4141 cgaagctcct caaggccttc accgccgagt ggggcgagcc ggagaaggtc gccgcgaacg
     4201 tcttcgtctg gaaggacgcg accgagacga tggtgatctt tcgcggcaca gacctcgagg
     4261 gctcggcgtc gagagccgag gtctacgtcg tctgcaagcc gctcgtcgag gcggcgaaca
     4321 aggccgaacg cgagcgccgc gagaagctgc tcggcatcaa gtagccgcga gccgcgccgc
     4381 agcgcggtcc gcgccgtcca gcgcgggcgc cttgccctgg aagacccggc ccgagccgcc
     4441 ccccttgccg ccgaggagcg ccgcgacttc gcggccgaga gcctggacgt cgccgaagcc
     4501 ctcgcccgcg ccgagcgcga aggcgtgctg ccctcccgcc gtcgccgtga cgagcgcggc
     4561 ccgcgctccc g
//
LOCUS       DAOMFY010000309         4536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6892_length_4536_cov_7.390091, whole genome shotgun sequence.
ACCESSION   DAOMFY010000309 DAOMFY010000000
VERSION     DAOMFY010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6892_length_4536_cov_7.390091"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PLB01_17495"
     CDS             <1..1256
                     /locus_tag="PLB01_17495"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ79147.1"
                     /translation="HAEPALEAELVAQVGIGDGRSAHVPWLRELPDPLTRVSWAACVR
                     VAPSRAAALGVKDGDHVTVDVSGRSVTLPARLLPGQNPRVLAVPVGFGRRDGDGGAAV
                     RNGYRLARLDGRLTTQGLAATAGRAAGHDALPLMQPHGSTEARPIVYQLGAFDEEVES
                     AAHEKAESLWPERLPTSPKWEMVIDLDACTGCSGCVVACQAENNLPVVGPDEMRRNRD
                     MYWLRIDRYFVGDEANPDVLFEPMLCAQCENAPCETVCPVAATVHSEDGLNQQVYNRC
                     VGTRYCANNCPYKVRRFNWFNHPVEAPVERLVLNPNVVARSRGVMEKCTFCVQRIQAT
                     RIAARRDGQQDWRGLGGKTACQESCPAKAIVFGDATDPRGEVAAWKKSPRAFQVLAEL
                     GVRPAVTYLAKVRTRTGGPVGKESA"
     gene            1253..2620
                     /gene="nrfD"
                     /locus_tag="PLB01_17500"
     CDS             1253..2620
                     /gene="nrfD"
                     /locus_tag="PLB01_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQQ79148.1"
                     /translation="MSAVAGGAADLVLGTPRFGEVTDEIARPMEVRPGRAWWAAFLAA
                     SSALTLGAVLIGYTIATGIGVWGLNRTVGWGYDITNFVFWVGIGHAGTLISAILLLFR
                     QRWRTSIARAAEGMTIFAVLCAALFPFIHMGRPWLAFWVFPYPNSRGPISVNFRSPLL
                     WDVFAISTYFTVSLLFWYVGLIPDLATMRDRSTGWRRRVYAFFAFGWDASTRTWQRYE
                     KACVLLAGLATPLVVSVHTIVSFDFATSVIPGWHSTIFPPYFVAGAVFSGFAMVITLL
                     LVTRRVLGLERYITAGHFDKMAKVLLTTGMIVGFAYATELAIAVYSVNRFERFTFENR
                     AFGPYAWAYWTMVFCNVAVPQVFWSARARRSALVLFAAACLVNVGMWFERFNIIVTSL
                     HRDYLPSSWSHYTPTLVEVGILLGSFGLFFTCFLLFVRVAPVVAFHEVKLTLAEQREA
                     AHAAH"
     gene            2607..3179
                     /locus_tag="PLB01_17505"
     CDS             2607..3179
                     /locus_tag="PLB01_17505"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HQQ79149.1"
                     /translation="MPRTDRVFPAASGSRLRVHRLVFDDPRLTLAAVGTLRAEGFEVD
                     DVHTPYAVHGMDEALGLRESRLGLATLAGGLAGCAIAFGFQIWTHAIDWPLVIGGKSP
                     LALPAQVPVSFELTILFAAFATVGALLVRRRLFPRLAVAPPGQPGPAVTDDRFVILVV
                     ERDGGFRPSRLAELASALGAIDPVEAWRVS"
     gene            3176..3736
                     /locus_tag="PLB01_17510"
     CDS             3176..3736
                     /locus_tag="PLB01_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQQ79150.1"
                     /translation="MSALRRVAALVAAAALAGCSAEGDRPGTVFLPDMVDSGVVHAYD
                     RSAVTRNGGALLLPPAGTVPVERTPFAYGPGPAEARRAGVELVNPFTASPEALARGKQ
                     VYDTICTVCHGAKGEGDGPIIGRFPNPPSLLAAHARTLPDGQIVHIVTRGQGIMPAHA
                     AQVLLEDRWRVALYLRQLQGAPEARP"
     gene            3733..>4536
                     /locus_tag="PLB01_17515"
     CDS             3733..>4536
                     /locus_tag="PLB01_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79151.1"
                     /translation="MTGPALSRLLRARPLEAGALGLLAAGVLVAGLFLDPPQAFAGLL
                     TAALFGLMLALGAAVFAAIQAVSGARWWAPVRTVASSAAGTLAVPAVLLGATLVLGLT
                     ALYPWARPEAAANHLLAAKAVWLNRPFFLARALAILLVWGGLVGLLRNRIRAVESGEA
                     GAAPRFARASVGFLLVLAPTISVACWDWAMSLEPEWFSTMFAVYGFAGTFLGGIAAVT
                     AVALFLDARGRLPQPLSPATRHDLGKLLFAFATFWAYIWFCQYLLIWYSN"
BASE COUNT          531 a         1720 c         1551 g          734 t
ORIGIN      
        1 cgcacgcgga gccggccctc gaggcggagc tcgtcgcgca ggtcgggatc ggcgacgggc
       61 ggagcgccca cgtgccgtgg ctgcgcgagc ttcccgaccc cctcacgcgc gtgtcctggg
      121 ccgcgtgcgt gcgcgtcgcc ccgtcgcggg ccgccgcgct cggcgtaaag gacggcgacc
      181 acgtgaccgt ggacgtgtcg ggccgctcgg tcaccctccc cgcacgcctc ctgccgggtc
      241 agaaccctcg tgtcctcgcg gtgccggtcg ggttcggccg ccgggacggc gacggcggcg
      301 cggcggtgcg caacggctac aggctggcgc gcctcgacgg ccggctcacg acacagggac
      361 tcgcggcgac ggccgggcgg gccgccggcc acgacgcgct gcctctcatg cagccgcacg
      421 gttcgaccga ggcgcgcccg atcgtctacc agctcggcgc cttcgacgag gaggtcgaga
      481 gcgccgcgca cgagaaggcc gagagcctct ggcccgaacg ccttcccacc tcgccgaagt
      541 gggagatggt gatcgacctc gacgcgtgca ccggttgcag cggctgcgtc gtcgcgtgcc
      601 aggccgagaa caacctgccc gtcgtcggcc ccgacgagat gcggcggaac cgggacatgt
      661 actggctgcg gatcgaccgg tacttcgtcg gcgacgaggc gaacccggac gtgctcttcg
      721 agccgatgct ctgcgcgcag tgcgagaacg cgccgtgcga gacggtctgc ccggtcgcgg
      781 ccacggtcca cagcgaggac ggcctcaacc agcaggtcta caaccggtgc gtcgggaccc
      841 gttactgcgc gaacaactgc ccgtacaagg tgcggcgctt caactggttc aaccacccgg
      901 tcgaggcgcc cgtggagcgc ctcgtcctga acccgaacgt cgtcgcccgc tcccgcggcg
      961 tcatggagaa gtgcacgttc tgcgtccagc ggatccaggc gacccgcatc gccgcccggc
     1021 gggacggcca gcaggactgg cgcggcctcg gcgggaagac cgcctgccag gagagctgcc
     1081 cggcgaaggc gatcgtgttc ggcgacgcca cggacccgcg cggcgaggtc gcggcgtgga
     1141 agaagagccc gcgcgccttc caggttctcg ccgagctcgg cgtcaggccc gcggtcacgt
     1201 acctcgcgaa ggtgcggacg cgcacgggcg gccccgtcgg aaaggagagc gcatgagcgc
     1261 cgtcgcagga ggcgccgcgg acctcgtcct cggaacgccc cggttcgggg aggtcacgga
     1321 cgagatcgcg cgcccgatgg aagtccgccc cggccgcgcc tggtgggccg ccttcctcgc
     1381 ggcgtcctcg gcgctcacgc tcggcgccgt cctgatcggc tacacgatcg cgaccggaat
     1441 cggcgtctgg ggcctcaacc ggaccgtcgg ctggggctac gacatcacga acttcgtgtt
     1501 ctgggtcggg atcgggcacg cggggacgct catctccgcg atcctcctcc tgttccgcca
     1561 gcgctggcgg acctcgatcg cccgcgccgc cgaggggatg acgatcttcg ccgtgctctg
     1621 cgcggccctc ttcccgttca tccacatggg ccgcccgtgg ctcgccttct gggtcttccc
     1681 ctacccgaac tcgcgcgggc cgatctccgt gaacttccgc tcgccgctcc tctgggacgt
     1741 cttcgcgatc tcgacgtatt tcacggtctc gctcctcttc tggtacgtcg gcctcatccc
     1801 cgacctcgcg acgatgcgcg accggagcac cgggtggaga cgccgcgtct acgcgttctt
     1861 cgccttcggc tgggacgcct cgacgcggac gtggcagcgc tacgagaagg catgcgtcct
     1921 cctcgcgggg ctcgcgacgc cgctcgtcgt ctcggtccac acgatcgtct cgttcgactt
     1981 cgcgacgagc gtcatccccg gctggcactc gacgatcttc ccgccgtact tcgtcgcggg
     2041 ggccgtcttc agcgggttcg cgatggtcat cacgctcctt ctcgtgaccc ggcgcgtgct
     2101 cggcctcgag cggtacatca cggccggcca cttcgacaag atggcgaagg tcctcctgac
     2161 gaccgggatg atcgtgggct tcgcctacgc gaccgagctg gcgatcgccg tctacagcgt
     2221 gaaccggttc gagcgcttca cgttcgagaa ccgcgccttc ggcccgtatg cgtgggccta
     2281 ctggacgatg gtcttctgca acgtcgccgt gccgcaggtc ttctggtccg cgcgcgcccg
     2341 gcgctccgcg ctcgttctct tcgccgccgc ctgcctcgtg aacgtcggga tgtggttcga
     2401 gcgcttcaac atcatcgtga cctccctcca ccgtgattac cttccctctt cgtggagcca
     2461 ctacacgccg acgctcgtgg aggtcgggat cctgctcgga agcttcggcc tcttcttcac
     2521 gtgctttctc ctcttcgtga gggtcgcgcc cgtcgtcgcg ttccacgagg tgaagctgac
     2581 gctcgccgag cagcgggagg cggcccatgc cgcgcactga ccgcgtcttc ccggccgcct
     2641 ccgggtcccg gctgcgggtc cacaggctcg tcttcgacga cccgcggctc acgctcgcgg
     2701 ccgtcggaac gctgcgggcc gagggcttcg aggtggacga cgtccacacg ccgtacgcgg
     2761 tccacggcat ggacgaggcg ctcggcctga gagagtcgcg cctcggcctc gcgacgctcg
     2821 cgggaggcct cgccggctgc gcgatcgcct tcgggttcca gatctggacg cacgcgatcg
     2881 actggccgct cgtgatcggc ggcaagtccc cgctcgcgct gcccgcgcag gtgccggtca
     2941 gcttcgagct cacgatcctc ttcgcggcct tcgcgacggt cggggcgctc ctcgtgcggc
     3001 gccgactctt cccgcggctc gcggtcgcgc cgcccgggca gcctggcccc gcggtcaccg
     3061 acgaccgctt cgtgatcctc gtcgtcgagc gcgacggcgg ctttcggccc tcgcgcctcg
     3121 ccgagctggc ctcggcgctc ggagcgatcg atcccgtcga agcctggagg gtttcgtgag
     3181 cgctttacga cgcgtcgccg cgctcgtcgc cgcggccgcc ctcgccggct gctccgcaga
     3241 gggcgaccgg cccggaacgg tgttcctgcc ggacatggtc gactcgggag tcgtccacgc
     3301 ctacgaccgg agcgcggtga cccggaacgg cggcgcgctc ctcctgccgc ccgccgggac
     3361 ggtcccggtg gagcggacgc ccttcgccta cgggcccggt cccgcggaag cgcggcgcgc
     3421 gggcgtggag ctcgtgaacc cgttcaccgc ctcgccggaa gcgctcgccc gcggcaagca
     3481 ggtctacgac acgatctgca cggtctgcca tggcgcgaag ggcgagggcg acggccccat
     3541 catcggccgc ttcccgaatc cgccgagcct cctcgccgcg cacgcgcgca cgctgccgga
     3601 cgggcagatc gtccacatcg tgacgcgcgg ccaggggatc atgcccgcgc acgcggcgca
     3661 ggttctcctc gaggaccgct ggcgggtcgc cctctacttg aggcagctgc agggcgcgcc
     3721 ggaggccagg ccgtgacggg gcccgccctc tcccggctgc tccgggcgag gccgctcgaa
     3781 gcgggggcgc tcggcctcct cgcggcgggc gtgctcgtcg ccggcctctt cctcgacccg
     3841 ccgcaggcgt tcgcgggcct cctgaccgcc gcgctcttcg gcctcatgct cgctctcgga
     3901 gcggccgtct tcgcggcgat ccaggccgtc tccggggcgc gctggtgggc tccggtccgc
     3961 acggtcgcct cgtccgcggc cgggacgctc gccgtccccg ccgtccttct gggagccact
     4021 ctcgtcctcg ggctcacggc gctctatccc tgggcgcgcc cggaggcggc cgcgaaccac
     4081 cttctcgccg cgaaggccgt ctggctgaac cgccccttct tcctcgcccg cgcgctcgcg
     4141 atcctcctcg tgtggggtgg gctcgtcggc ctcctccgga acaggatccg cgccgtcgag
     4201 tcgggggagg ccggagccgc tccgcgcttc gcgcgcgcct ccgtggggtt ccttctcgtc
     4261 ctcgccccga cgatctcggt ggcctgctgg gactgggcga tgtctctcga gcccgagtgg
     4321 ttcagcacga tgttcgcggt ctacgggttc gcggggacct tcctcggcgg aatcgcggcc
     4381 gtcaccgccg tcgcgctctt cctcgacgcg cgcgggcgcc tgccgcagcc cctctcgccc
     4441 gcgacgcgcc acgacctcgg caagctcctc ttcgccttcg cgacgttctg ggcgtacatc
     4501 tggttctgcc agtacctcct gatctggtac agcaac
//
LOCUS       DAOMFY010000310         4533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6903_length_4533_cov_4.994640, whole genome shotgun sequence.
ACCESSION   DAOMFY010000310 DAOMFY010000000
VERSION     DAOMFY010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6903_length_4533_cov_4.994640"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PLB01_17520"
     CDS             <1..1510
                     /locus_tag="PLB01_17520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ79152.1"
                     /translation="VVGTGLLGRAVAGPAAGLAAAAVLAVEPTFRGHGALVQSDVLLT
                     LFLVAAAFALEKGGEGDRRWLAAGGVLYGLAMASKYSAFPFLAVFAAVAVLRLGTKST
                     GRFSSKGKRGAPQRLPARSRKWERAAGARREAPSSPSKKFFSSLSLVFVYLVVPALLT
                     LALVQLLAYAGTTDTAFRRGLGAAFEGLPQEGAVVALAKTFPKWIAGYGAGLLFVQGV
                     AGPGERFNYFFGVVRGTGSLLYFPVALGIKLTATAVLASLAALVFAVAGFFRGPIFRR
                     LLAARAALPAALGGAYLLAACVSNVNIGVRHALPAVPFLLVAAAASGRALLAMRPRAF
                     RALAAAVVLLAAAESALRLGREISFGNVLVGGPAGVPPILSDSNVDWGQEQGLVFERV
                     KRGDLGRVAIASLLVDETGATRAGILGLALRADADADTVFFSRFLWDLAAAIEKNGDA
                     YSKFVWLRGWLPPLRRGLEARATSVAPFGDAYLLLRLRPATAAPPPAPGSTP"
     gene            complement(1470..3437)
                     /locus_tag="PLB01_17525"
     CDS             complement(1470..3437)
                     /locus_tag="PLB01_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79153.1"
                     /translation="MTVHTEDVPRAAGLAAVGVLAVLLTTVDGRSYGLIPDGREMLSA
                     GTAVARFLEIGVSRDFVNAPRRSGGDAVSRYGMGLSLVEAVPAVLARATGSSRTSPLF
                     VLVPILSLVGCAWAAARALGLLGAGRIPAAAGGAALVLATPLWGYAGSDYGEPLQALC
                     VALSVLAVVELREGPAARRWQVVLGAAAGFAILTKTLLAIVVAPLLLCAVVGRKGKEA
                     EKDFLEGHGAGEPHRGRAGRAARRRAVRGDGPRAPRLALPFPLLFTFIGILFLWSLFE
                     FVRFGTLGGGYGGETFTYPFFTGLLRLTLLPNKGLLWYAPLAVLAIPGFLLLRRRDAP
                     LALALAASTLALLLASSAWWAWDGQAGWGPRLVVPALPCLAALAGVAFTGGGRAWRAG
                     GACALAAGVCVNALGALVPFPAVYALSSQVPPQPISESRAAGTPYEIERGADGVLRAT
                     APHHLSLTPAWSPIRVHALALAARLKGGWDGSLPQLDPPFRPAPSPSPAPAFRLALAS
                     PHPGFWRPGDSGWADPYFDALRDQLVRAIDTRDFARAASLGEELTKREEKLSSNDPRT
                     IALVAEALRLGSGAAVSLSYLSKGSSLLSSSSPSCHSWILFVRAMAEPPGDFSCLPEA
                     QREAFAGAVAVARQRGWTLTEWNRALAAGRP"
     gene            complement(3461..3910)
                     /locus_tag="PLB01_17530"
     CDS             complement(3461..3910)
                     /locus_tag="PLB01_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79154.1"
                     /translation="MRNPTCSGSTFATLLAAALILVPAALPFPAPAQAPDVGVLEVSV
                     DPAFPAAFGQELKLRVRVSNRGTAAAKGVALDAKAGAIAVGHVDVGSMGAGEQRTIML
                     ATRFKPAGADCVSVVPVVAPDSPVKAGPSRLACLTPGCYSLSDRPGN"
     gene            complement(4042..>4533)
                     /locus_tag="PLB01_17535"
     CDS             complement(4042..>4533)
                     /locus_tag="PLB01_17535"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ79155.1"
                     /translation="PWAAVALGALAVFVAVGWRVRRAAVLRAAAPPPVLAAGELPAAG
                     DDPIERLKLGLPSRLREDLTAETLAEIAGVSLRTLQRIFRERFDTTPMSWLMEARLLE
                     AARVIRAGDDPVTKIAYRVGFKDPSHFTRRFKARFGVSPNEYRRGGEAEPEPPAGGSG
                     DAV"
BASE COUNT          668 a         1673 c         1600 g          592 t
ORIGIN      
        1 cgtcgtcggg acgggactcc tcggccgcgc cgtcgccggc ccggcggccg gcctcgcggc
       61 ggcggccgtg ctcgcagtcg agccgacctt ccgcggccac ggcgcgctcg tccagagcga
      121 cgttctcctg acgctcttcc tcgtggcggc cgcgttcgcg ctggagaagg gcggggaggg
      181 cgatcgtcgc tggctcgccg cgggcggcgt cctctacggc ctcgcgatgg cgtcgaagta
      241 ctcggcgttc ccgttcctcg ccgtcttcgc ggcggtcgcc gttctccggc tcggcacgaa
      301 gagcaccggg agattttctt cgaagggtaa gaggggcgcg ccgcagcgcc tccccgcgcg
      361 aagccgcaag tgggagcggg cagccggcgc acgccgcgag gcgccctctt caccttctaa
      421 gaaattcttc tcttctcttt ctctcgtctt cgtctatctc gtcgtccccg ctcttctcac
      481 gctcgccctc gtccagctcc tcgcgtacgc cggcacgacc gacaccgcgt tccggagggg
      541 cctgggcgcc gcgttcgaag gcctgccgca ggagggcgcc gtcgtcgccc tcgcgaagac
      601 gttcccgaag tggatcgcgg gatacggcgc cggcctcctc ttcgtacagg gcgtcgcggg
      661 ccccggcgag cgcttcaact acttcttcgg cgtcgtgcgc ggaacgggct cgctcctcta
      721 cttccccgtc gcgctcggaa tcaagctcac ggcaacggcc gtcctcgcgt cgctcgccgc
      781 cctcgtcttt gccgtcgcgg gcttcttccg cggcccgatc ttccgccgcc tgctcgccgc
      841 gcgggcggcg ctccccgccg cgctgggagg cgcatacctg ctcgccgcct gcgtctcgaa
      901 cgtgaacatc ggcgtccgac acgctcttcc cgccgttccc ttccttctcg tcgccgcggc
      961 cgcttccggc cgcgcgctcc tcgcgatgcg cccgcgggcg ttccgcgccc tcgccgcggc
     1021 cgtggtgctc ctggccgcgg ccgagtcggc cctccgcctc gggcgcgaga tctcgttcgg
     1081 aaacgtcctc gtgggcggac cggccggcgt tccgccgatc ctctcggact caaacgtcga
     1141 ctgggggcag gagcaggggc tcgtcttcga gcgcgtgaaa cgcggcgacc tcggaagggt
     1201 cgcgatcgcg tccctcctcg tcgacgagac gggagcgacg cgcgccggca tcctcggcct
     1261 cgcgctgagg gcggacgccg acgccgacac ggtgttcttc tcccggttcc tctgggatct
     1321 cgccgccgcc atcgagaaga acggcgacgc gtattcgaaa ttcgtctggc tgcgggggtg
     1381 gctgcctccg ctgcggcgcg ggctcgaggc gcgcgcgacg tcggtcgcgc cgttcggcga
     1441 cgcgtacctt ctcctgcgtc tccggcccgc tacggccgcc ccgccgccag cgcccggttc
     1501 cactccgtga gcgtccagcc gcgctgacgc gcgaccgcga ccgcgcccgc gaaggcctcg
     1561 cgctgcgcct ccggcaggca cgagaagtcg cccggcggct cggccatcgc gcggacgaag
     1621 aggatccagg agtggcaaga aggggaagag gacgaaagaa gagaagatcc cttcgaaagg
     1681 taagagaggg agacggcggc gcccgaaccg aggcgcagcg cctcggcgac cagcgcgatc
     1741 gtgcgagggt cattcgaaga aagcttctct tctctcttcg tgagctcttc tccgagggag
     1801 gccgcccgcg cgaagtccct cgtgtcgatc gcccggacga gctgatcccg cagtgcgtcg
     1861 aaatagggat cggcccagcc ggaatcgccg gggcgccaga aacccgggtg cggcgaagcg
     1921 agcgcgaggc gaaacgcagg cgcgggtgac ggcgatggcg cgggccggaa cggcggatcg
     1981 agctgcggga gactgccgtc ccagccgccc ttcaggcgcg cggcgagcgc gagcgcgtgg
     2041 acgcggatgg gcgaccatgc cggggtcagc gaaaggtggt gcggcgccgt tgcgcgcagc
     2101 acgccgtccg ctccgcgctc gatctcgtac ggcgtgcccg cggcacgcga ttcggagatc
     2161 ggctgcggcg ggacctgcga agaaagcgca tagaccgcgg ggaacggcac gagcgcaccg
     2221 agcgcgttca cgcagacgcc tgccgcgagg gcgcacgctc ccccggcacg ccatgcacgg
     2281 ccgcctcccg tgaacgcgac cccggccagg gctgcgaggc acggcaaagc cggcacaacg
     2341 aggcgcggtc cccaccccgc ctgcccgtcc cacgcccacc aggcggaaga cgcaaggagc
     2401 agcgcgagcg tggacgccgc gagcgcgagc gcaagcggcg cgtcgcggcg acgcagcagc
     2461 aggaaacccg ggatcgcgag gaccgcgagc ggcgcgtacc agagaagccc cttgttcggg
     2521 agaagggtca gccgcagcaa gcccgtgaag aagggatacg tgaacgtctc tcccccgtac
     2581 ccgcccccca gcgtcccgaa tcttacgaat tcgaagagag accagagaaa gagaattcct
     2641 atgaaggtga agagcagggg gaatggcagc gcaaggcgag gagcccgagg cccatccccg
     2701 cggaccgcgc gcctgcgcgc cgcccgcccc gctctgccac gatgcggctc accagcccca
     2761 tgaccttcta agaagtcttt ctctgcctct tttcccttcc ttccgacgac cgcacacaga
     2821 aggagcggcg cgacgacgat cgcgagcagc gtcttcgtca ggatggcgaa gccggccgcg
     2881 gcgccgagga cgacctgcca gcggcgcgcc gccggccctt cgcgcagctc gacgactgcg
     2941 aggacggaaa gcgccacgca gagcgcctgc aggggctcgc cgtagtcgct ccccgcatag
     3001 ccccagagcg gcgtcgcgag gacgagcgcc gcgccgcccg ccgcagcggg gatcctgccg
     3061 gcgccgagaa ggccgagcgc ccgcgccgcc gcccacgcgc aacccaccag ggacaggatc
     3121 ggaacgagga cgaagagcgg cgacgtccgg gatgagcccg tcgcgcgcgc aagcaccgcc
     3181 gggaccgctt cgacgaggga gaggcccatt ccgtagcgcg acaccgcgtc cccgccggag
     3241 cgccggggcg cgttcacgaa gtcgcgcgag acgccgatct cgagaaagcg cgccacggcg
     3301 gtcccggccg agagcatctc ccttccgtcc gggatcaggc cgtacgagcg gccgtccacc
     3361 gtcgtgagga ggacggcaag gacgcccacc gccgcgagcc ccgccgcccg cggcacatcc
     3421 tcggtgtgga ccgtcacgcc cggattctac gagcgggcgt tcagttcccg ggccggtcgg
     3481 acaggctgta acagcccggc gtcaggcacg cgaggcggct cggcccggcc ttcaccggcg
     3541 agtcgggcgc gacgacgggc acgacggaaa cgcagtccgc gccagccggc ttgaagcgcg
     3601 tcgcgagcat gatcgtgcgc tgctcgccgg cgcccatcga gccgacatcg acgtgaccga
     3661 ccgcgatcgc tcccgccttc gcgtcgaggg cgacgccctt cgcggcggcc gtgccgcggt
     3721 tcgagacgcg aacccggagt ttgagctcct gtccgaaggc cgcggggaac gccgggtcga
     3781 cgctgacctc gagaacgccc acgtcgggcg cctgggcagg agcgggaaac ggcagggcgg
     3841 ccgggacgag gatcagggcc gcggcgagga gcgtggcaaa cgtcgagccg gagcaggtgg
     3901 gattccgcat ctcgtctcct gtgctcaagg tgctctcttc gcggccggcc gtccaccccg
     3961 cccggtggtc ccgacgcgct aatcgcgcgt cccggagcgc cagaggcccg ctcttgcccc
     4021 tgagcgacag attccgggcc ctcacacggc gtccccggag ccgcccgctg ggggctccgg
     4081 ctccgcttcc ccgccccgac ggtactcgtt cggcgagaca ccgaaccggg ccttgaaccg
     4141 gcgtgtgaag tgcgacgggt ccttgaagcc gacgcgatac gcgatcttcg tgacggggtc
     4201 gtcgccggca cggatgacgc gcgcggcctc gaggagccga gcctccatga gccagctcat
     4261 cggggtcgtg tcgaagcgct cccggaagat gcgctggagc gtgcgcagag aaacgcctgc
     4321 gatttccgcg agcgtctcgg ccgtgaggtc ctcgcgcagg cgggacggga ggccgagctt
     4381 gagccgctcg atcggatcgt cgccggccgc gggcagctcg cctgccgcaa gaaccggcgg
     4441 gggcgccgcg gcccggagaa cggcggccct ccgcacgcgc cagcccacgg ccacgaagac
     4501 cgcgagcgca ccgagcgcca ctgccgccca ggg
//
LOCUS       DAOMFY010000311         4508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_6959_length_4508_cov_6.815405, whole genome shotgun sequence.
ACCESSION   DAOMFY010000311 DAOMFY010000000
VERSION     DAOMFY010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_4508_cov_6.815405"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLB01_17540"
     CDS             <1..501
                     /locus_tag="PLB01_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79156.1"
                     /translation="QARVYLDGNYIGISDDWDDAGGGSLLTFNLEGQHRLRICYPERR
                     DLLVDVIVRSTAADDKVTVEQDLPKGTPDGPTGPEGSFRRPNYKSIGAVLFNVEPPDA
                     MVTVNGKEYGPASKWQKEEMTFANMAVYQVALSAPGYETKTVRVLIAPTAGELRATVK
                     ERLKKQ"
     gene            complement(502..1419)
                     /locus_tag="PLB01_17545"
     CDS             complement(502..1419)
                     /locus_tag="PLB01_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQQ79157.1"
                     /translation="MGRAQDADFLAGARPRRVFAAGDEIRDGGSPAGAFLFLEGAAEL
                     LAETPFGPHPVARLAAPALADLRAALGGASGVARLRPEPGTAAVFLSAEDARTLAFDP
                     GDAGGAFRRIALASLTTAIRDTNASLARFFEGLSVKPVPPDPTRAHTFDSDLDLARAR
                     DLFETAGLDPAILPALGLTARTVMPGAALVKKGDTGHEAFLLVEGRLRVSLDIAGAGE
                     EALAILKPGEIVGEMGLVDDAPRSADVYAHGGPAVVYVLTRDVFRRLLGSGAPEGAPL
                     LGGLTSLLARRLEEAIKKAAGFRILSGPI"
     gene            1497..2612
                     /gene="gcvT"
                     /locus_tag="PLB01_17550"
     CDS             1497..2612
                     /gene="gcvT"
                     /locus_tag="PLB01_17550"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQQ79158.1"
                     /translation="MSGPSSDSAQVLRRTPLWERHRALGGKMVPFGGWDMPVEYSGLS
                     EEHRAVRERAGLFDVSHMGEIRVKGPKAFEVVQHLTCNDVAALDDGKVQYSAFLTPQG
                     TFVDDLLTYRLAADDYLLVVNASNTPKDVAWAKAAACAGASVEDESPIWAQLALQGPK
                     AETILQTLTETPLSRITYYRFEPGKVLGVPALLSRTGYTGEDGFELYLHPDHAGDVWD
                     AILKAGRPHGILPAGLGARDTLRLEACLALYGNDIDDAHTPWEANLGWIVKMKKGDFQ
                     GREALAKQKEAGVARKLVGFEVTGRGIARHGYPLTVDGAAGVVTSGTQTPTVGKPIGL
                     AYVPAEKTAPGTVLAIDVRGRGVPAVIVPTPFYRRRT"
     gene            2609..3004
                     /gene="gcvH"
                     /locus_tag="PLB01_17555"
     CDS             2609..3004
                     /gene="gcvH"
                     /locus_tag="PLB01_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQQ79159.1"
                     /translation="MSNYPDDRRYTRSHEWIRVDGDVGTIGISDHAQKELGEIVFVEL
                     PDVGEIFDAGQEFGTIESVKAVSEIFLPVAGEILETNKVLADEPGAVNEDPHGDGWLV
                     KVKLTSDAEVDALMNAADYEKFVEEEAKA"
     gene            3064..4422
                     /gene="gcvPA"
                     /locus_tag="PLB01_17560"
     CDS             3064..4422
                     /gene="gcvPA"
                     /locus_tag="PLB01_17560"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQQ79160.1"
                     /translation="MRYIPNSPDERAELLAAVGASSVDDLFATVPRAVYHREAPPLPP
                     PASEIEVRRDFGALAASNASLSEWTSFLGAGLYSHHAPAFVSQLLLRGEFLTAYTPYQ
                     PEVSQGTLTAVYEFQTHVALLTGMDIANASMYEGASAFVEAVLMAERLTKRKSKVVVS
                     QGIHPEYLRCLRTYLVNFPFTIVEVPVGADGATDAAALAAAVDDATFAVAVQSPNAFG
                     VVESWDALAAAAHAKNALAIGVVNEMFSLALLQPPGARGVDIACGEAASFGVPPSFGG
                     PLVGFLACRDAFKRQIPGRLVGKTVDADGAPAFCLTLSTREQHIRREKATSNICTNQG
                     LFALAATMTLSALGKQGLRETALQCLAKAEYLKAGVKALAGPFSLAFSGKTFNEFALR
                     CGPGVDAVLRRAEEHRILAGVPYARLDPSGSAHGDLLLVAVTERVPRAGLDGFLAVLR
                     SF"
BASE COUNT          682 a         1616 c         1593 g          617 t
ORIGIN      
        1 caggcgcgcg tctatctcga cggaaactac atcgggatct cggacgactg ggacgacgcc
       61 ggaggcggct cgctcctgac gttcaacctc gagggccagc accggctccg gatctgctat
      121 ccggagcgcc gcgatctcct cgtggacgtc atcgtccgct cgacggcggc cgacgacaag
      181 gtcacggtcg aacaggacct cccgaaggga acgccggacg gcccgacggg tcccgaaggc
      241 agcttccgcc ggccgaacta caagtcgatc ggtgccgtcc tcttcaacgt ggaaccgccg
      301 gacgcgatgg tcacggtgaa cggcaaggag tacggcccgg cgtcgaagtg gcagaaggaa
      361 gagatgacgt tcgcgaacat ggccgtctac caggtcgccc tctcggctcc gggctacgag
      421 acgaagacgg tccgcgtcct gatcgccccg acggccggcg agctccgcgc gacggtcaag
      481 gaacggctga agaagcagta gtcagatcgg cccggaaagg atccggaagc cggccgcctt
      541 cttgatcgcc tcctccaggc gccgcgcgag gagggacgtc aggccgccga ggaggggagc
      601 gccttcgggc gcgccgcttc cgagcaggcg ccggaagacg tcgcgcgtga ggacgtagac
      661 gaccgcgggc ccgccgtgcg cgtagacgtc cgccgaacgc ggcgcgtcgt cgacgaggcc
      721 catctcgccc acgatctcgc cgggcttgag gatcgcgagg gcctcctcgc cggcgccggc
      781 gatgtcgagg gagacgcgga gcctgccctc gacgaggaga aacgcctcgt gtccggtgtc
      841 gcccttcttc acgagcgccg cgcccggcat cacggtgcgc gccgtgaggc cgagcgccgg
      901 gaggatcgcc gggtcgagac ccgcggtctc gaaaaggtcg cgcgcgcggg ccaggtccag
      961 gtcggagtcg aacgtgtggg cgcgcgtcgg gtccggaggc acgggcttga ccgagagccc
     1021 ctcgaagaac cgcgcgagcg aggcgttcgt gtcgcggatc gcggtcgtca ggctcgcgag
     1081 tgcgatccga cggaacgctc cgcccgcgtc gccggggtcg aacgcaaggg tgcgggcgtc
     1141 ctccgcggac aggaaaacgg ccgcggtccc cggttccggc cggagccggg cgacgccgga
     1201 tgcgcccccg agggccgcac ggaggtcggc gagggcgggg gccgcgaggc gcgcgacggg
     1261 atgcgggccg aacggcgtct ccgcgaggag ctcggccgcg ccctccagga acaggaatgc
     1321 accggccggc gaaccgccgt cccggatctc gtctcccgcg gcgaagactc gtcggggccg
     1381 cgcccccgcg aggaaatcgg cgtcttgtgc gcgtcccacc ccgggattat ccgccgctcg
     1441 gtgcacgtgc atcggttgac ggggcggggg gcgggatcta tcgtcccggc gcggcgatga
     1501 gcggtccctc ttcggattcg gcccaggtcc tcaggcgcac gccgctctgg gagcggcacc
     1561 gcgcgctggg cggcaagatg gttccgttcg gcgggtggga catgcccgtg gagtactccg
     1621 ggctgtccga ggagcaccgc gccgtccgcg agagggccgg actcttcgac gtctcccaca
     1681 tgggcgagat ccgcgtcaag gggccgaagg cgttcgaggt cgtccagcac ctcacgtgca
     1741 acgacgtcgc ggcgctcgac gacggcaagg tccagtattc cgcgttcctg acgccgcagg
     1801 ggacgttcgt cgacgatctc ctgacgtacc ggctcgccgc ggacgactac ctcctcgtcg
     1861 tgaacgcgtc gaacacgccg aaggacgtcg cgtgggcgaa ggccgccgcg tgcgcgggcg
     1921 cttccgtcga ggacgagtcg ccgatctggg cacagctcgc gctccagggg cccaaggcgg
     1981 agacgatcct ccagacgctc accgagaccc cgctttcccg gatcacgtac taccgcttcg
     2041 agcccggcaa ggtcctcggc gttcccgcgc ttctctcgcg caccggttac acgggcgagg
     2101 acggcttcga gctctacctg cacccggacc acgcgggcga cgtgtgggac gcgatcctga
     2161 aggcgggccg gccgcacgga atcctgcccg cggggctcgg cgcacgcgac acgctgcgcc
     2221 tcgaggcctg cctcgcgctc tacggcaacg acatcgacga cgcgcacacg ccgtgggaag
     2281 cgaacctcgg ctggatcgtc aagatgaaga agggcgactt tcagggccgc gaggcccttg
     2341 cgaagcagaa agaggcgggc gtcgcgcgga agctcgtcgg cttcgaggtc acgggccgcg
     2401 ggatcgcccg gcacggctat ccgctcacgg tggacggggc cgcgggcgtc gtgacgtccg
     2461 ggacgcagac gccgacggtc ggcaagccca tcggcctcgc ctacgttccc gcggagaaga
     2521 ccgcgccggg caccgtcctg gcgatcgacg tgcggggccg cggcgtcccg gccgtcatcg
     2581 ttccgacgcc gttctacagg agaaggacat gagcaactac ccggacgacc gccgctacac
     2641 gaggagccac gagtggatcc gcgtggacgg cgacgtcggc acgatcggca tcagcgacca
     2701 tgcccagaag gagctcggcg agatcgtgtt cgtcgagctg ccggacgtcg gcgagatctt
     2761 cgacgccggg caggagttcg ggacgatcga gtccgtgaag gcggtctccg agatcttcct
     2821 gcccgtcgcc ggcgagattc tcgagacgaa caaggtcctc gccgacgagc ccggcgccgt
     2881 gaacgaggac ccgcacggcg acggctggct cgtgaaggtc aagctcacgt ccgacgccga
     2941 ggtcgacgcc ctcatgaacg cggctgacta cgagaaattc gtcgaggaag aggccaaggc
     3001 ctgaccccgc ggacgtccgg cgccgcctcc ccggcgtaaa gcggacaggg aaggaacgtt
     3061 tcgatgcgct acatcccgaa cagtcccgac gagcgcgccg agctgctcgc cgccgtcgga
     3121 gcctcgtcgg tcgacgacct cttcgcgacc gtcccgcgcg ccgtctatca ccgtgaggcg
     3181 ccgccgctcc cgccgcccgc ctccgagatc gaggtccgcc gcgacttcgg cgccctcgcc
     3241 gcctcgaacg cgtcgctgtc cgagtggacg tccttcctcg gcgccggcct ctacagccac
     3301 cacgcgccgg cgttcgtctc gcagctcctc ctgaggggcg agttcctgac ggcctacacg
     3361 ccctaccagc ccgaagtctc gcaggggacg ctcacggccg tgtacgagtt ccagacgcac
     3421 gtcgcgctcc tgacgggcat ggacatcgcg aacgcctcga tgtacgaagg ggcgtccgcc
     3481 ttcgtcgagg cggtgctcat ggccgagcgc ctcacgaaga ggaagtcgaa ggtcgtcgtc
     3541 tctcagggca tccaccccga gtacctccgg tgcctccgga cgtacctcgt gaacttcccg
     3601 ttcacgatcg tcgaggtccc cgtcggcgcg gacggcgcga ccgacgcggc ggcccttgcg
     3661 gccgccgtgg acgacgcgac gttcgccgtc gccgtccagt cgccgaacgc gttcggcgtc
     3721 gtcgagagct gggacgctct cgcggccgcg gcccacgcga agaacgcgct cgcgatcggc
     3781 gtcgtgaacg agatgttctc gctcgcgctg ctccagccgc ccggagcccg cggcgtcgac
     3841 atcgcctgcg gcgaggcggc ctccttcggc gtcccgccct cgttcggggg cccgctcgtg
     3901 ggcttcctcg cctgccgcga cgcgttcaag cgccagatcc ccgggcgtct cgtcggcaag
     3961 acggtggacg cggacggcgc ccccgcgttc tgcctgacgc tctcgacgcg cgagcagcac
     4021 atccggcgcg agaaggcgac gtcgaacatc tgcacgaacc agggcctctt cgcgctcgcc
     4081 gcgaccatga cgctctcggc gctcggcaag cagggcctcc gcgagacggc gctccagtgc
     4141 ctcgcgaagg cggagtacct caaggccggg gtgaaggccc tcgcggggcc gttctcgctc
     4201 gcgttctccg ggaagacctt caacgagttc gccctgcgct gcggcccggg cgtggacgcg
     4261 gtgctgcgcc gcgccgagga gcacaggatt ctcgcgggcg tcccgtacgc gcgcctcgac
     4321 ccgtcgggct ccgcgcacgg cgacctcctt ctcgtcgccg tgaccgagcg tgtcccgcgc
     4381 gccgggctcg acggcttcct cgccgtcctg aggagcttct gagatgagcg acgcctccga
     4441 tcgtgccacc ctcgcagccg aaccgccggc ccgggcggac gcgcgcctcg accggcgccg
     4501 ccgcttcg
//
LOCUS       DAOMFY010000312         4461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7081_length_4461_cov_7.058783, whole genome shotgun sequence.
ACCESSION   DAOMFY010000312 DAOMFY010000000
VERSION     DAOMFY010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7081_length_4461_cov_7.058783"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="PLB01_17565"
     CDS             <1..1752
                     /locus_tag="PLB01_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79161.1"
                     /translation="ARAPQAPAPSAGLTASLLPPEGWTFDPLGGPPVLSPDGTRLAFM
                     VSDSSRTQLCVRSLATGEMSILPGTETGRNPFWTVDGRNIGFMGQTTGLATVPASGGA
                     VEFLAPSGPGRGGTAAKDGRILYSPALLSGIFLVEPGRAGRVQATKLDEARGESAHQW
                     PHFLPDGRHFLYVVQRVDAATRRAESELCVQALGEDTRKVLVKSATRAIYVPGYLLYS
                     WNGSLMAQAFDAENLATAGDPFVVAPRVEYLADGGTGIFAASAQGLLVYAVGGTIGNS
                     QLVVYDRSGKVVRTVGETGNYWSPRVSPDGKLVASEVIDPVSSNRDVWTFDVAGSDPP
                     VRRTFDPGEDYTPVFSPDGKRIAYSGFRKGEWAIYEKSLAGGDDERVVARAARSGSPA
                     PPAGSGTLLGRGSKFLTAWSPDGRYVAFNNSTQETLDDMWVLSVEDGSPREILRTPAS
                     ERDTAFSPDGRWVAYMSGETGRPEVYVRAFAGAGGKWQISTGGGGAPRWSRDGRELFY
                     LGAGGRLMAAPVKTGAAFEKGAPRELFLVGLRRTNIPQYDPFPDGQRFIANAVVTEKT
                     SPPLTLVQNWSSLVKTK"
     gene            1749..3407
                     /locus_tag="PLB01_17570"
     CDS             1749..3407
                     /locus_tag="PLB01_17570"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HQQ79162.1"
                     /translation="MRSAALALALSVAAPALADDLQALSRDFWAWRAVTKPMDRDDVG
                     RVVRPHGWTPDWSLESIETRRRTLALFEERWVKVADASKPVPWQVDRRLIGSALARVR
                     WEIDVKRSWRRDPTFWVDQTLPAVVDALLSPPPFEKERSEDVVLRLESIPRTLDEARA
                     ALDEAAAPFAKLAIEDLRVGGPKLKASTAALSPLLAPDARGRVAGATSAAVAALDSFR
                     VWLEARLPLLAERKPVGRDGYVFFLRNVALMPFTPEELLLMGRQEYERAVALEVYERQ
                     RNAGAAEIPMAADQAAQSAMTTRAEAEIRRFLEEKGVLSVPAWMPHYVDLPVPAYLDA
                     LDGFGEWTLFANTARTGEPSIRWIPKPSPKLGYFAGSMARDRRPHMSHEGIPGHAFQL
                     ALAARHDDEIRRAFYDSGPIEGLAFYSEELLQSMGLYADAPKTREMVANYMRLRALRV
                     EVDVKLALGTFTIPQAAEYLRTSVPMDAETARAEAAAFASSPGQAITYQIGKLQVQRL
                     LAEAKRAQGDAFRLRAFHDFVYRNGNVPLSLLRWELLGQEDDLK"
     gene            3404..>4461
                     /locus_tag="PLB01_17575"
     CDS             3404..>4461
                     /locus_tag="PLB01_17575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79163.1"
                     /translation="MTLAAGTRLGNYEIVAQIGAGGMGEVYRARDPRLNRDVAVKILP
                     KNFLEGEESRSRFEREAKLLAALNHPSIAAIYSFEEISGSSTSSSSPRHVLVMELVEG
                     ESLRDRLRAGALAPKKAVELASQLAEGLAAAHLRGIVHRDVKPENILLNREGRVKILD
                     FGLAKQLPKWAGPSGPHTNLPTEAPSGARTEAGVVMGTVGYMSPEQVKGEPADHRSDI
                     FSFGAVLYEMLCGRKAFGGASGVETMNAILNEDPPALVVTKGQMPPALERVVLHCLEK
                     SPENRFQSMKDVAFDLGSISTISAVDGGKAGARSRGRARWVAAGLAAAVLVGLGAWAT
                     GVVHLGRHEQPVFHQVSFR"
BASE COUNT          645 a         1561 c         1590 g          665 t
ORIGIN      
        1 gctcgcgccc cgcaagcgcc ggctccatcg gcgggtctca cggcgtcgct cctgccgccc
       61 gaaggctgga ccttcgatcc gctcggcggg ccgccggtgc tctcgccgga cgggacgcgg
      121 ctcgccttca tggtctccga ctcgagtagg acccagcttt gcgtccgttc tcttgcgacc
      181 ggcgagatgt cgatcctccc cggcacggag accgggcgca acccgttctg gaccgtcgac
      241 ggccggaaca tcgggttcat gggacagacg acagggctgg cgacggtgcc ggcctccggc
      301 ggcgccgtcg agtttctcgc cccgagcggg ccgggccgcg gcggcacggc ggcgaaggac
      361 ggcaggatcc tgtattcccc ggcgctcctg agcgggatct ttctcgtcga gccgggccgg
      421 gcggggcgcg tccaggcgac gaagctcgac gaggcccgag gcgagtccgc tcatcagtgg
      481 ccgcacttcc ttccggacgg ccggcacttc ctgtacgtcg tccagcgcgt cgacgcggcc
      541 acgcgccgcg ccgagagcga gctgtgcgtt caggcgctgg gggaggacac acggaaggtc
      601 ctcgtgaagt ccgcgacacg ggcgatctac gtcccgggct acctcctcta ttcctggaac
      661 ggctcgctga tggcgcaggc gttcgacgcc gagaacctcg cgacggccgg ggatcccttc
      721 gtcgtcgcgc ctcgggtcga gtacctcgcc gatggcggca cgggcatctt cgcggcttcc
      781 gcgcagggac tcctcgtcta tgccgtgggc ggcacgatcg gcaactcgca gctcgtcgtc
      841 tacgaccgct ccgggaaggt cgtgcgcacc gtcggcgaga ccggcaacta ctggtcgccg
      901 cgcgtctcgc ccgacgggaa gctcgtcgct tccgaggtca tcgaccccgt gagcagcaac
      961 cgcgacgtct ggacattcga cgtcgccggc tcggaccccc cggtccgccg gaccttcgac
     1021 cccggggagg actacacgcc cgtgttctcg ccggacggga agaggatcgc ctacagcgga
     1081 ttccgcaagg gcgagtgggc gatctacgag aaatctctgg cgggcggaga cgacgagcgc
     1141 gtcgtggcgc gggcggcgcg gtccgggtcg ccggcgccgc cggccggttc cgggacgctc
     1201 ctcggtcgcg gctcgaagtt cctgaccgcc tggtcccccg acggacggta cgtcgcattc
     1261 aacaactcga cacaggaaac gctggacgac atgtgggttc tctccgtcga ggacggctcg
     1321 ccgcgcgaaa tcctgcggac gcccgcttcc gaacgggaca cggccttctc gccggacggg
     1381 cgctgggtcg cgtacatgtc cggcgagacg ggccgtccgg aggtctacgt gcgcgcgttc
     1441 gcgggagccg gcgggaagtg gcagatctcg accggcggcg gcggtgcgcc gcgctggagc
     1501 cgggacggac gggagctctt ctacctcgga gcgggcgggc ggctgatggc cgcgcccgtg
     1561 aagacggggg ccgcgttcga aaagggcgcg ccccgcgagc tcttcctcgt cggtttgcgc
     1621 cgcacgaaca tcccgcagta cgaccctttc cccgacggcc agaggttcat cgccaacgcg
     1681 gtcgtgacgg aaaagacctc gcccccgctg acactcgtcc agaactggag ttcactggtg
     1741 aagacgaaat gagatccgct gcgctcgccc ttgccctgtc cgttgcggcg ccggcccttg
     1801 ccgacgacct gcaggccctc tcccgcgact tctgggcgtg gcgcgcggtc acgaagccga
     1861 tggaccgcga cgacgtgggc cgcgtcgtcc gccctcacgg ctggacgccc gactggtcgc
     1921 tggagtccat cgagacgcgg cgcaggacgc tcgcgctctt cgaggagcgc tgggtgaagg
     1981 tcgccgacgc gtcgaagccc gtcccgtggc aggtggaccg gcggctcatc ggctccgcgc
     2041 tcgcacgcgt tcgctgggag atcgacgtca agcgctcgtg gcgccgggac cccacgttct
     2101 gggtggacca gaccctgccg gccgtcgtgg acgctcttct ctcgccgccg ccgttcgaaa
     2161 aggagcgcag cgaagacgtc gtcctccggc tcgagtcgat cccgcggacg ctcgacgagg
     2221 cgcgcgcggc gctcgacgag gccgcggcgc cgttcgcgaa gctcgcgatc gaggaccttc
     2281 gcgtcggagg gccgaagctg aaggcgtcga cggcggcgct ctctccgctc ctcgcgccgg
     2341 acgcacgcgg gcgcgtcgcc ggggccacga gcgcggcggt cgcggcgctg gactcgttcc
     2401 gcgtctggct cgaggcccgc ctgcccctgc tcgcggagcg caagccggtc ggccgcgacg
     2461 gatacgtctt ctttctccgg aacgtcgccc tcatgccgtt cacgccggag gagcttctcc
     2521 tcatgggccg gcaggagtac gaacgcgccg tcgcgctcga ggtctacgaa cgacagcgga
     2581 acgcgggcgc ggcggagatc ccgatggccg cggaccaggc cgcccagtcc gcgatgacga
     2641 cccgcgcgga ggccgagatc cgccgattcc tcgaggagaa gggggtcctc tccgtgccgg
     2701 cgtggatgcc gcactacgtg gaccttcccg tgccggcgta cctcgacgcg ctcgacggct
     2761 tcggcgaatg gacgctcttc gcgaacacgg cgcgcacggg agagccctcg atccgctgga
     2821 tcccgaagcc gtccccgaag ctcgggtatt tcgcggggtc gatggcgagg gaccgccgcc
     2881 cgcacatgtc gcacgagggc attccgggcc acgccttcca gctcgcgctc gcggcgcgcc
     2941 acgacgacga gatccggcgc gcgttctacg actcggggcc gatcgaaggg ctcgcgttct
     3001 actcggagga gctccttcag tcgatggggc tctacgcgga cgctcccaag acgcgcgaga
     3061 tggtcgcgaa ctacatgcgc ctcagggcgc tgcgcgtgga agtggacgtg aagctcgcgc
     3121 tcgggacgtt cacgatcccg caggccgcgg aataccttcg aacgagcgtc ccgatggacg
     3181 ccgagacggc gcgcgccgag gcggccgcgt tcgcgtcgtc gcccggccag gcgatcacgt
     3241 accagatcgg gaagctccag gtgcagcggc tgctcgccga ggcaaagcgc gcgcagggcg
     3301 acgcgttccg gctgcgcgcg ttccacgact tcgtctaccg taacggcaac gtgccgctct
     3361 cgctccttcg atgggagctg ctgggacagg aggacgacct gaaatgacgc tcgccgccgg
     3421 gacccgcctc gggaactacg agatcgtcgc ccagatcggc gcgggcggta tgggagaagt
     3481 ctaccgggcg agggacccgc gcctgaaccg cgacgtcgcg gtgaagatcc tgccgaagaa
     3541 tttcctggag ggtgaagaat cgcgctcgcg cttcgagcgg gaggcgaagc tgcttgccgc
     3601 gctgaaccac ccttcgatcg cggcgatcta ttcattcgaa gaaatctccg gttcttcaac
     3661 ttcttcgtct tcgcctcggc acgttctcgt catggagctg gtcgagggtg agtcgctccg
     3721 ggaccggctg agggcgggcg cgctggctcc gaagaaggcc gtggagctgg cctcgcagct
     3781 ggccgagggc ctcgcggccg cgcatctgcg cggcatcgtc catcgcgacg tgaagccgga
     3841 gaacatcctc ctgaaccgcg agggccgggt gaagatcctg gacttcggcc tcgccaagca
     3901 gctcccgaag tgggcgggac cgagcggccc gcacacgaac ctcccgacgg aggccccttc
     3961 gggcgcgcgc acggaggcgg gcgtcgtcat gggaaccgtc ggctacatga gcccggagca
     4021 ggtgaagggc gagccggccg accaccggag cgacatcttc agcttcggcg ccgtgctcta
     4081 cgagatgctc tgcgggcgga aggcgttcgg cggcgccagc ggcgtcgaga cgatgaacgc
     4141 gatcctcaac gaggaccctc ccgccctcgt cgtcacgaag ggccagatgc cgccggcgct
     4201 cgaacgcgtc gtcctgcact gcctcgagaa gtcgcccgag aaccggttcc agtccatgaa
     4261 ggacgtcgcg ttcgacctgg gctccatctc gacgatctcc gccgtggacg ggggcaaggc
     4321 cggggcgcgg tcccgcgggc gcgcgcgctg ggtggccgcc gggctcgccg ccgcggtcct
     4381 cgtgggcctg ggggcgtggg cgacgggagt cgtgcacctg ggccgccacg agcagcccgt
     4441 cttccaccag gtctcgttcc g
//
LOCUS       DAOMFY010000313         4453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7094_length_4453_cov_5.350841, whole genome shotgun sequence.
ACCESSION   DAOMFY010000313 DAOMFY010000000
VERSION     DAOMFY010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7094_length_4453_cov_5.350841"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLB01_17580"
     CDS             complement(<1..357)
                     /locus_tag="PLB01_17580"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ79164.1"
                     /translation="MLRFVLRRAASALLTLLGVLALVFGLLAAAPGDPAAMAAKAGAR
                     ASAVSPEALAAFRTLYGLNRPPLERFGIWILRAARFDFGISLSDGRPVPARISGALPA
                     TLTLNLAALLAAVALGV"
     gene            complement(383..1966)
                     /locus_tag="PLB01_17585"
     CDS             complement(383..1966)
                     /locus_tag="PLB01_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ79165.1"
                     /translation="MRRLAAAAMLLALAACGVRGKDQTSVIRRRLDGEPRTLNPILAT
                     TDAEQIVVALLSRNLLDYDENLNLVPGLAEEVSDSPDHLVFTVRLRPDARWEDGTPVT
                     SADVVFTLNAVMDPKVPALARRSFFEGFVKAEAVDVRTARVTFRTPSAGRRHAFNIWL
                     LSAAAWKDGDLATHPRNRSPLANGPYRLGSWEPGRTLSLVRNTQYAGPAPPAEQVVFR
                     VVPETAPAFAGLLAGDLDEMRLTFAQKKEVDAKAGSPGAPRAVTFDELSYAYFGWSNR
                     SPLFSDRRVRRALTMLIDRESISRNLFGGLAKLANGPLPPAHWAWNAAIAPWPHDPSQ
                     AERLLDEAGFRKGPDGIRHKGSLRFAFTFSLGMGSDVQRQMVELAQQSFRKAGIDMKI
                     QPLEWAAFAAKMDAGELEAWAAALNLDPYPDLGVSWHSAQTPPTGLNNAFYRNPDVDA
                     LLDRLRSTFDRAEAIRLLGEVQRLVHEDEPVSFLNTPLTKWGVSGRIEGVRTSPYGLF
                     LFWPGASAWRPGVGSRPIS"
     gene            complement(1963..4176)
                     /locus_tag="PLB01_17590"
     CDS             complement(1963..4176)
                     /locus_tag="PLB01_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79166.1"
                     /translation="MKRLFASFALALAAASSPLVAATSRVAITAGARDFLAGEAKGTA
                     VSADGRLTLAPPLAPRAWPGDAADAAVFAAAGDASGRVFVATGGGLGRLFVSDAKGVT
                     LLFTAPEPNLTAVVVAPDGSVVCASSPNGKIYRVNPKESDPAKAGTLLADPREAAIWA
                     LAFGPDGTLYAGTGNKGRVYRKTAAGALELFHEIEDVHVRTLLAGPDGTVYAGTSDRG
                     LVVAIGAKGARTLHDFSRPEVTGLALDGKGVLYAAASQADASAGRAVAAELRLRPTPT
                     PTPTPGPAEETPKGSVSIAATTAAARPSSAASSANNSEIVAVAPDGFVEPAWTFPEEA
                     VFALRFDAASGSLLVATGPRGRLYALKDRSLRLLAQTGEKLVVSAPAAGAAFAAVTNG
                     AAGILRPAATAAAGSFVSAVKDAVRLSTFGRVRWEGATPPGTSVALSVRSGTSEKPDA
                     TWSAWSPAGAAGEAKVPVARFFQWKADLSPNAKGESPSVERVDMTYAERNARPVLENV
                     AVLEPGVVFGRAGSPGAGVLSVTNPDENGIFAGLDQPRDGTPSEGPGRRLWRKGFRTV
                     TWKGTDPNGDVLRYEVEARRESGPWFPVRKEIEESFLSFDTTALADGKYRFRVTASDR
                     LSQPEGDALTASEESSVALVDNTPPVLKLESRKEESGAVELRILATDALSPVVRAEAS
                     VNADRWRILQAEDGAADSPVERFVFRVPKPAGAFVLGVRVVDAAGNVATLSVEGP"
     gene            complement(4173..>4453)
                     /locus_tag="PLB01_17595"
     CDS             complement(4173..>4453)
                     /locus_tag="PLB01_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79167.1"
                     /translation="RSLAGLRRMVERLLPANRLVATLLVPARGATTGGATLTALPPSA
                     AAILSDGEGSDLGRSAIASRLLAEDILTLDRPVAGTIRIEVEIERPRS"
BASE COUNT          632 a         1574 c         1677 g          570 t
ORIGIN      
        1 tacgccgagt gcgaccgcgg cgaggagcgc ggcgagattg agcgtgagcg tcgcgggcag
       61 cgcgcccgag atccgcgccg ggacgggccg cccgtcagac agggagatgc cgaagtcgaa
      121 acgcgcggcg cgcaggatcc agattccgaa gcgctcgagg ggcggacggt tcagtccgta
      181 gagggtccgg aaggccgcca gcgcctcggg cgagaccgcg gacgctcgcg cgcccgcctt
      241 cgcggccatc gccgcgggat ccccgggcgc cgccgcgagc agcccgaaaa cgagcgcgag
      301 gacgcccagc aacgtcagca gggccgacgc cgcccgcctg aggacgaacc ggagcatgcg
      361 ggaatgttac ttggggggcc cgtcaggaga tcggccggga gcccacgccg ggccgccacg
      421 cggacgcgcc gggccagaac aggaagagcc cgtatggcga agtccggact ccctcgatgc
      481 gtcccgagac gccccacttc gtgagcgggg tgttcaggaa cgacacgggc tcgtcctcgt
      541 ggacgaggcg ctggacctct ccgagcaggc gaatggcctc tgcacggtcg aaggtggagc
      601 gcaggcggtc gaggagcgcg tcgacgtccg gattccggta gaacgcgttg ttgaggcccg
      661 tcggcggggt ctgagccgaa tgccacgaga cgccgaggtc ggggtacggg tcgaggttga
      721 gcgccgcggc ccacgcctcg agctcgcccg cgtccatctt cgcggcgaac gcagcccact
      781 cgagcggctg gatcttcatg tcgatccccg ccttccggaa cgactgctga gcgagctcga
      841 ccatctggcg ctggacgtcg ctccccatcc cgagcgaaaa cgtgaaggcg aaacgaaggc
      901 ttcccttgtg ccggatgccg tcgggtccct tgcggaaccc cgcctcgtcg aggagccgct
      961 cggcctggga aggatcgtgc ggccacggcg cgatcgcggc gttccacgcc cagtgggcgg
     1021 gcggcagcgg cccgttcgcg agcttcgcga ggccgccgaa gaggttacgg ctgatgctct
     1081 cgcggtcgat cagcatggtg agcgcgcggc gcacgcgccg gtccgagaac agcggcgagc
     1141 ggttgctcca gccgaagtac gcgtacgaga gctcgtcgaa cgtgaccgcg cgcggggctc
     1201 ccggactccc ggccttcgcg tccacctcct tcttctgggc gaacgtgagg cgcatctcgt
     1261 cgaggtcgcc ggccaggagg ccggcgaacg ccggcgccgt ctcggggacg acgcgaaaca
     1321 cgacctgctc ggcgggcggc gcggggcccg cgtactgcgt gttgcgcacg agcgagagcg
     1381 tccgccccgg ctcccacgat ccgagccggt acgggccgtt cgcgagcggg ctgcggttgc
     1441 gcgggtgcgt cgcgaggtcg ccgtccttcc acgccgccgc ggaaaggagc cagatgttga
     1501 acgcatgccg ccggcctgcg gacggagtcc ggaacgtgac ccgcgccgtt cgaacgtcga
     1561 cggcctccgc cttcacgaac ccctcgaaga acgagcgccg cgcgagcgcc ggcaccttcg
     1621 gatccatcac cgcgttcagc gtgaacacga cgtcggcgga cgtcacgggc gtcccgtctt
     1681 cccagcgggc gtccggcctc aggcgcacgg tgaagacgag gtggtcgggt gaatccgaga
     1741 cctcctcggc gaggcccggg acgaggttca ggttctcgtc gtagtcgagg aggttccgcg
     1801 acaggagcgc gacgacgatc tgctccgcat ccgtcgtcgc gagaatcggg ttcagggtcc
     1861 tcggctcgcc gtcgagccgc cggcggatca cgctcgtctg gtctttcccg cgcacgccgc
     1921 acgcggcgag cgcgaggagc atggcggcgg cggcgagtcg tctcacggcc cttccaccga
     1981 cagcgtcgcg acgttgccgg ccgcgtcgac gacgcgtacg ccgaggacga acgcccctgc
     2041 gggcttcggc acgcggaaca cgaaccgctc cacgggcgag tcggcggcgc cgtcctcggc
     2101 ctgcaggatc ctccagcggt cggcgttcac ggaggcctcg gccctgacga ccggcgaaag
     2161 cgcgtccgtc gccaggatcc ggagctcgac cgcgccgctt tcctccttgc ggctctcgag
     2221 cttgaggacc ggcggcgtgt tgtcgacgag agcgacggag ctctcctccg atgccgtgag
     2281 agcgtctccc tcgggctgcg agagccggtc ggaggccgtg acgcggaaac ggtactttcc
     2341 gtcggcgagc gcggtcgtgt cgaacgaaag gaacgactcc tcgatttcct tgcggaccgg
     2401 gaaccagggc ccgctctcgc gccgcgcttc cacctcgtag cgcagaacgt cgccgttcgg
     2461 gtcggtccct ttccaggtga ccgttcgaaa gcccttccgc cagagccggc ggccggggcc
     2521 ctcggacggg gtcccgtcgc gcggctggtc gagccccgcg aagatcccgt tctcgtccgg
     2581 gttcgtgacg gagaggacgc cggcgccggg cgagcccgcc cggccgaaga cgacgcccgg
     2641 ctcgaggacg gcgacgttct cgaggaccgg ccgcgcgttc cgctcggcgt acgtcatgtc
     2701 cacgcgctcg acggacggag actcgccctt cgcgttcggc gagaggtcgg ccttccactg
     2761 gaagaaccgc gcgacgggga ccttcgcctc gccggcggcg ccggcggggg accaggccga
     2821 ccacgtcgcg tccggctttt cgctcgtccc ggaacggacc gagagcgcca cggaagtgcc
     2881 gggcggcgtc gcgccctccc atcggacccg gccgaaggtc gacaggcgaa cggcgtcctt
     2941 cacggcggag acgaacgagc cggccgccgc cgtggccgcg ggccgcagga ttccggcggc
     3001 gccgttcgtc acggccgcga acgcggcgcc cgcggcgggc gccgagacga cgagcttttc
     3061 gccggtttgc gcgaggaggc gcagagagcg gtccttcagg gcgtagaggc ggcctcgcgg
     3121 ccccgtcgcc acgaggagcg aaccgctcgc ggcgtcgaag cgcagcgcga agacggcctc
     3181 ctccgggaac gtccacgcgg gctcgacgaa gccgtccggc gcgaccgcga cgatctcgga
     3241 gttgttcgcc gacgacgccg ccgaggacgg gcgcgccgcg gcggtcgtcg ccgcgatcga
     3301 gacgcttccc ttcggagttt cctcggcggg gcccggcgtc ggcgtgggcg tcggcgtcgg
     3361 cctgaggcgc agttcggccg cgaccgcccg gcccgcggag gcgtcggcct gcgacgccgc
     3421 ggcgtagagc acgcccttgc cgtccagcgc gaggccggtg acctcgggcc tcgagaagtc
     3481 gtgcagggtg cgcgcgccct tcgcgccgat cgcgacgacg agcccgcggt cggacgtgcc
     3541 cgcgtagacg gtcccgtccg ggccggcgag cagcgtccgg acgtgcacgt cctcgatctc
     3601 gtggaagagc tcgagagcgc ccgccgccgt cttgcggtac acgcggccct tgttgccggt
     3661 cccggcgtac agcgtcccgt ccgggccgaa cgcgagcgcc cagatcgccg cctccctggg
     3721 atcggcgagg agcgtcccgg ccttcgcggg gtcgctctcc ttcggattca cccggtaaat
     3781 cttcccgttc ggcgacgacg cgcagacgac ggagccgtcc ggcgcgacga cgacggccgt
     3841 gaggttcggc tccggggcgg tgaagaggag cgtcacgccc ttcgcgtcgg acacgaagag
     3901 ccggcccagc cctccgcccg tcgcgacgaa gacgcgcccc gaggcgtcgc ccgccgccgc
     3961 gaagaccgcg gcgtcggcgg cgtccccggg ccatgcgcgc ggggcgagcg gcggcgcgag
     4021 cgtgagacgg ccgtccgcgg agaccgcagt gcccttcgcc tctccggcga ggaaatcccg
     4081 ggcgcccgcc gtgatggcga cccgcgacgt ggcggcgacg agcggggacg aggctgcggc
     4141 gagcgcgagc gcgaagctgg cgaagagtcg tttcaagagc gcggcctttc gatctcgacc
     4201 tcgatccgga tggttccggc cacggggcgg tcgagggtga ggatgtcctc ggcgaggagc
     4261 cgggaggcga tggcagagcg gcccaggtca gagccctcgc cgtccgagag gatcgcggcc
     4321 gcggacggcg gcagggcggt cagcgtggcg ccgcccgtgg tggcgccgcg cgcggggacg
     4381 aggagcgtcg cgacgagccg attcgcggga aggagccgct cgaccatgcg gcgcagcccg
     4441 gcgagactgc gcg
//
LOCUS       DAOMFY010000314         4380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7295_length_4380_cov_5.575954, whole genome shotgun sequence.
ACCESSION   DAOMFY010000314 DAOMFY010000000
VERSION     DAOMFY010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7295_length_4380_cov_5.575954"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLB01_17600"
     CDS             complement(<1..574)
                     /locus_tag="PLB01_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79168.1"
                     /translation="MNKSLGSLVAVSLLASAASAADAPGGVLFPAAFSVEHQLVQTDP
                     GGDTFHAQPVVDTYGGSTLVSVRPGGSRVIVDFGKRTVTEVSVEKSTYWTLSFSQMGD
                     LSRRLAKADERPSTPSAASPRAANAAVKQDIRVEDVTESGRERLAAPGGPASRAGVRH
                     LRASVANGPSADVWVDPTVRMSPAALDVVAT"
     gene            complement(571..>2375)
                     /locus_tag="PLB01_17605"
     CDS             complement(571..>2375)
                     /locus_tag="PLB01_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79169.1"
                     /translation="DSGGALKGTWRIGSVTNSTVERSLPGDWGNSLQDGVVYDGYLIA
                     SDIRVVDHPELVGHINYNADSRTFGARYSGGQWQYDNNSTFIPFTPHASDRVFATYKG
                     GVSNIVRLSCPLGVCPSVNGIPVLAMVAGDIFPNFDPWSQNTSEIWVSNPVFSTTLAR
                     FTLVPNGAETISIVPGDKYQGVYRFDVAPTVKGAATLTSADPIRVGGTLLQAEPVAAA
                     ATAAPAAGRPLSLCTDFAAPLKPGSSFVACADTGARDVSLEISGAFEAHLPGYGNCHD
                     PIAIPGSARPGPLKIVATARDAEGRTVSAAAHALVVADERAPVLVSVEPAPGASFRSG
                     DPLRVAVEAWDDVGIASVAITLGGTRTVLTAPPFEVVALAPPVAAGGSLPVHVEVFDP
                     SGNVSRRALDLGVLPSGARLPRTGPVAPEPGVSFEDGRLAVDGGWPWRDADGETRGRT
                     LELPAIGTHAVLAVDGAVVALDGPVARAAVHGGAVDVRRGDDIVGRFRILSASEDGTV
                     LRLEGGAAGDVRRGDFLEGNWMFDSVRLTRGAILRAADAVEARSLHVDASSLLLSKNL
                     RIPSPDPPVAECPARGPRPDPPGTAPVSAPGVAP"
     assembly_gap    2376..2475
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2476..4135)
                     /locus_tag="PLB01_17610"
     CDS             complement(<2476..4135)
                     /locus_tag="PLB01_17610"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ79170.1"
                     /translation="MANASGKPAWPTPFSAAQRFGSVEGRGAARLVFTDDLTVGADPG
                     VVNDRASIVLDAEARLLLKADAPQIALTASPDGGNVTTGQPLSVSYTLSDPLGLFARV
                     ATFTPSGAVTAVFADEPVTVSSGVLPLQVPANQPPGPVTYSLQVRDRAGRTQSVSRSW
                     TVLPDAPPVLNLTGVTPGPSVLPGAVIAATANATDDVGLTSVVFTLSGAVTASDPRPV
                     AGLSSAQAFSTRLPTTLTAGQTVTLQVDAADTFGHKTSAAPVTWTILADTAPPSIAPV
                     AISPVKAGDVYTTGDVVTFTVTATDNVAVAAIRLTIDGVTTPFAGSPAVLTWTAPNVG
                     GSTPYTLDVEALDVAGNRATASRAVSVVPAGGGTFPMVSFACPTAGAFLPSAYAGLLL
                     SAAATDAGGVTKIEFFRPGDVTPFSTSTATGAPVSYTGTSTAVTLPTVAADTVVRYRA
                     RAWNPSNHFTEVFVDVHVVPAVDFASATATDFAVLRSGTAVVDAPKTFAGLIVLRGAT
                     ITHTAATGPGGERAASLTVAGPMYVECGASVDVSGRGYGSTTTYP"
     gene            complement(4138..>4380)
                     /locus_tag="PLB01_17615"
     CDS             complement(4138..>4380)
                     /locus_tag="PLB01_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79171.1"
                     /translation="PRRTGWVQLQALQGLGFSELKFGVLDLSPEVQAAIGRSQSARPA
                     GGTPSLRTRTRSRSGARARSTSRSWTRSARRRRRAD"
BASE COUNT          636 a         1555 c         1536 g          553 t
ORIGIN      
        1 tggtcgcgac cacgtcgagc gccgcgggcg acatgcgtac tgtcgggtcc acccagacgt
       61 cggccgacgg cccgttcgcg acggacgcgc gcaaatgccg cacgccggcg cgcgacgcgg
      121 gcccgcccgg ggccgcgagg cgctcccgtc cggactccgt gacgtcctcg acccggatgt
      181 cctgcttcac cgccgcgttc gcggcgcgcg gcgacgccgc ggaaggcgtc gacggccgct
      241 cgtcggcttt cgcgaggcgg cgcgagaggt cgcccatctg cgagaacgac agcgtccagt
      301 acgtggactt ctccacgctg acttccgtga ccgtgcgctt gccaaagtcc acgatgacgc
      361 gcgaaccgcc cgggcggacc gagacgagcg tcgacccgcc gtacgtgtcc acgaccggct
      421 gcgcgtggaa cgtgtcgccg ccgggatcgg tctggacgag ctggtgctcg acggagaagg
      481 ccgccgggaa gaggacgccg cccggagcgt cggccgcaga agcggccgac gcgagaaggg
      541 aaaccgcgac gagcgaaccc aagctcttgt tcatggcgcg acccccgggg cggacaccgg
      601 cgcggtgcct ggcggatccg ggcgcgggcc acgcgccggg cactcggcga ccgggggatc
      661 cggagaggga attcggagat tcttggaaag taagagggac gaggcgtcga catgcaggct
      721 tctcgcctcg accgcatccg ccgcacgcag gatggcgccc ctcgtgagcc taacgctgtc
      781 gaacatccaa ttaccttcta agaaatcccc tcttcgaacg tcacccgctg ctccgccctc
      841 gaggcgcagc accgtcccat cctccgaagc agaaagaatc ctgaaccgcc cgacgatgtc
      901 gtccccgcgc cgcacgtcca ccgcgccgcc gtgcaccgcg gcgcgcgcga ccggaccgtc
      961 gagcgcgacg acggcgccgt ccacggcgag caccgcgtgc gtgccaatgg ccggcagttc
     1021 cagagtcctg ccgcgcgtct cgccgtcggc gtcgcgccac ggccagccgc cgtccacggc
     1081 gagcctgccg tcctcgaacg agacgcccgg ttcgggcgcg accggcccgg tccgcggcag
     1141 ccgcgcgccg gaaggcagga cgccgaggtc gagcgcgcgg cgcgagacgt tgcccgacgg
     1201 gtcgaagacc tccacgtgga cgggcagcga cccgccagcc gcaaccggcg gcgcgagcgc
     1261 gacgacctcg aacggcggcg ccgtcaggac cgtcctcgtt cccccgagcg tgatcgcgac
     1321 ggatgcaatg ccgacatcgt cccacgcctc gaccgccacg cgcagcgggt cgcccgagcg
     1381 gaaggacgcc ccgggcgcgg gctcgacgga cacgagcacc ggcgcgcgtt cgtcggcgac
     1441 gacgagagcg tgcgcggcgg cggacaccgt ccgcccctcg gcgtcgcgcg cggtcgcaac
     1501 gatcttcagc ggccccggcc gggccgagcc cgggatcgcg atgggatcgt ggcagttgcc
     1561 gtagccgggg aggtgcgcct cgaacgcccc ggagatctcg agcgagacgt cccgcgcgcc
     1621 cgtgtcggcg cacgccacga acgaggagcc cggcttgagc ggcgccgcga agtcggtgca
     1681 gagggagagc gggcggccgg cggctggtgc ggccgtcgcc gccgcggcga cgggctcggc
     1741 ctgcagcaac gttccgccga cgcggatcgg atccgcgctc gtgagcgtgg cggcgccctt
     1801 gaccgtgggc gccacgtcga agcggtagac gccctggtac ttgtcgccgg gcacgatcga
     1861 gatggtctcg gctccgttcg gcacgagggt gaagcgcgcc agggtcgtcg agaacaccgg
     1921 attgctcacc cagatctcgc tcgtgttctg cgaccaggga tcgaagttcg ggaatatgtc
     1981 tcctgcgacc atcgccagca ccggaatccc gttcacgctc gggcacacgc ccagcgggca
     2041 cgacaggcgc acgatgttcg agacgcctcc cttgtacgtc gcaaacaccc tgtcgctggc
     2101 gtgcggcgtg aagggtatga acgtgctgtt gttgtcgtac tgccactgcc cgcccgagta
     2161 ccgcgccccg aacgtccgcg agtctgcgtt gtagttgatg tgccccacca gttcggggtg
     2221 atccacgacc cggatgtccg acgcgatgag atagccgtcg tagacgaccc cgtcctgcag
     2281 ggaattgccc cagtcgcccg gaagactcct ctcgacggtt gagttcgtga cgcttccgat
     2341 gcgccacgtc cccttgagcg cgccgcccga gtcccnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnccggg tacgtcgtcg tcgagccgta cccccgcccc gacacgtcca
     2521 cggacgcgcc gcattccacg tacatcggcc cggcgacggt caggctcgcc gcgcgctcgc
     2581 cgcccggccc ggtcgcggcc gtgtgcgtga tcgtcgcccc gcgcaggacg atgagccccg
     2641 cgaacgtctt cggcgcgtcg acgacggccg tgcccgagcg cagcaccgcg aagtccgtcg
     2701 ccgtcgcgct cgcgaagtcc accgccggga ccacgtggac gtcgacgaag acctcggtga
     2761 agtggttcga cgggttccag gcgcgggcgc ggtatcggac gaccgtgtcg gccgcgaccg
     2821 tcggcagcgt tacggcggtg gacgtgcccg tgtacgacac gggcgcgccg gtcgcggtgg
     2881 aggtcgagaa tggcgtcacg tcgcccgggc ggaagaactc gatcttcgtg acgccgccgg
     2941 cgtcggtcgc ggccgccgag agcagcaggc cggcataagc cgacggcagg aacgccccgg
     3001 cggtcggaca cgcgaacgac accatcggga acgtcccgcc gcccgcgggc acgacgctca
     3061 cggcccgcga tgccgtggcc cggtttccgg cgacgtcgag cgcctcgacg tcgagggtgt
     3121 acggcgtcga gcccccgacg ttcggtgccg tccacgtgag aacggccgga gagccggcga
     3181 acggcgtcgt cacgccgtcg atcgtcaggc gaatcgcggc aaccgcgacg ttgtcggtag
     3241 cggtgaccgt gaacgtgacg acgtcgccgg tcgtgtagac gtcgcccgcc ttcacggggg
     3301 agatcgcgac gggcgcgatc gacggcggcg ccgtgtccgc caggatcgtc cacgtcacgg
     3361 gcgcggccga cgtcttgtgg ccgaacgtgt cggccgcgtc cacctgcaac gtcacggtct
     3421 ggccagccgt gagcgtcgtc ggcagcctgg tgctgaacgc ctgcgccgaa gagagtcccg
     3481 ccaccggacg cgggtcgctc gccgtgacgg cgccggagag cgtgaagacc accgaggtca
     3541 gcccgacgtc gtccgtggcg ttggccgtcg ccgcgatcac cgcgcccggc aggacggacg
     3601 ggccgggcgt cacaccggtc aggttgagca ccggaggcgc gtcgggcagg accgtccacg
     3661 atctcgaaac ggactgcgtg cgtcccgcgc gatcgcggac ctgcagcgag tacgtgaccg
     3721 gcccgggcgg ctggttcgcc ggaacctgca gcggaagtac tccgctcgag accgtcaccg
     3781 gctcgtcggc gaagacggcc gtcacggcgc cggacggagt gaacgtcgcg actcgtgcaa
     3841 acaggccgag cggatccgag agcgtgtacg agaccgagag cggctgcccg gtcgtcacgt
     3901 tccctccgtc ggggctcgcg gtcagcgcga tctgcggcgc gtccgccttc agcagcagcc
     3961 gcgcctcggc gtcgagcacg atcgaggcgc ggtcgtttac gacgcccggg tcggcgccga
     4021 cggtgaggtc gtccgtgaag acgagcctcg cagcgccgcg gccctcgacc gagccgaacc
     4081 gctgcgccgc cgagaacggc gtcggccacg cgggcttgcc cgaagcgttc gccacgatta
     4141 gtcggcccgg cgccttcggc gcgccgagcg cgtccagctc ctcgatgtag accgtgcccg
     4201 cgcccccgat ctgctgcgcg ttcgcgtccg gagggagggc gttccgcccg ccgggcgcgc
     4261 cgactgcgag cggccgatgg ccgcctgcac ctcggggctg aggtcgagca cgccgaactt
     4321 gagttcgctg aaacccaggc cctgcagcgc ctgcaactgc acccagccgg tgcgccgcgg
//
LOCUS       DAOMFY010000315         4346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7395_length_4346_cov_7.901655, whole genome shotgun sequence.
ACCESSION   DAOMFY010000315 DAOMFY010000000
VERSION     DAOMFY010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7395_length_4346_cov_7.901655"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLB01_17620"
     CDS             complement(<1..1177)
                     /locus_tag="PLB01_17620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ79172.1"
                     /translation="MTQFRPDRRRTLLAAERSTESLSRHGRTRVALGFPNSYEIGMSN
                     LGFQWVYRLFNREDDVACDRFFVEDDGDLRGPRTLETDTPLGDFPLVAFSISWEMDYV
                     NFLRLLPAARIPLDRAERGEGDPIVLVGGDCARINPAPLAQWVDAFAMGDGEKIVPAI
                     AEILRRDLSREGTVAALADVKGLYVPAVHGARAEGADEGKIVVQQFKAGEGPLREPPH
                     TTILTPHTELSDKLLIEIARGCSEMCRFCWAAYAMAPQVRVSAEHVLEVARRNRPLTS
                     RVGLIATAVADHPEILPILRGLQALEYHIALSSVKIDAISEEILGILTAQGERALAIA
                     PEAGNERLRRAINKKVSDEMLFEKVRLVARSGITQLKLYLQVGLPGETDADVDDIVGL
                     "
     gene            complement(1158..2102)
                     /gene="cysK"
                     /locus_tag="PLB01_17625"
     CDS             complement(1158..2102)
                     /gene="cysK"
                     /locus_tag="PLB01_17625"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQQ79173.1"
                     /translation="MIHDSILGTIGNTPVVRINRLAPAGTTMYVKCEAFNPMSSVKDR
                     LAIAMIEDAERRGVLKPGQTVIEPTSGNTGIALAMVCAVKGYPFVAIMADSFSVERRK
                     LMKGLGAKVILSPAANRGSGMVKQARELADKHGWFLPQQFENPANPEYHRNTTGPEIL
                     RDFAGKRLDYFVSGLGTGGTITGAGEILKAARPDIKIVFTEPAAAPMVTEGKWAPHKI
                     QGWTTDFVPAVFNRKVYDEAVLVTDVESRDAARELAQKEGIFCGLSSGATFAAARKVA
                     EKAPKGSVLLAMLPDTGERYLSTILFEGITEDSDPIPA"
     gene            2147..>4346
                     /locus_tag="PLB01_17630"
     CDS             2147..>4346
                     /locus_tag="PLB01_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79174.1"
                     /translation="MKSRVSVSTIAAGVVLAAVAFASPARAQGGVSEWPREFTTPKGN
                     TIVMYQPQAETFQGDRATGRAAVSFLKKGEAAPKFGVIFFSARVSVDRDTRLVSVLGM
                     KVSRVRFPGITPEGEKKFTDNLEAEVPKWDLVFSYDRLLENVKVAQREKKSAEGLRHD
                     PPKIVFADEPSVLLSYDGDPVFREVEGAPVKVAVNTSLLVVNDTRSGRYYLSGGRKWW
                     YEAASPLGPWRTVGGPPADIAAFAAQAQAEQKKDDADTTGEDGTEGAAPPRIVVATEP
                     TEIVVTEGKPALKPIAGTDGSLLAVDNTEGDVLFDVGTQDYYVLLSGRWFKGKSLASG
                     GAWTYVAPDALPADFAKIPPESDSGDVRAAVAGTDEAEDAVLDAQIPQTTAVKRAEAN
                     LQVQWDGEPKFIAITGTATSYAANASTSVLLIRGRYYACENAVWFVSDSAEGPWTVAD
                     SVPSDDIDAIPPSEPVYNVKYVRIYDATPDLVYFGYTPGYTGIYPWHGTVVWGTGWTY
                     RPWVGPTYWWPRPYTWGLCARYNPWTGWGFGFSWSYPFFSVSFGWGGWFRPRGWYGPA
                     YGSWHRPGGWGWYGPGGYRPPAVIAGHYWGAHGGGTWNRPLPNTRPGAVRPAVRPLPG
                     VRPGIGFRPPTKDIRPPAQSNIYNRLPARSREVPRPPSTFERPKISTGRPNNVYADPS
                     GEIYRRTKEGQWQQRESGRWKPSSGGATARPGGTGPATRPPAGQPPSVTTR"
BASE COUNT          641 a         1537 c         1540 g          628 t
ORIGIN      
        1 cgaggccgac gatgtcgtcg acgtccgcgt ccgtctcgcc gggcagcccg acctgcaggt
       61 agagcttcag ctgcgtgatg cccgagcggg cgacgaggcg caccttctcg aacagcatct
      121 cgtcggagac cttcttgttg atcgcgcggc ggaggcgctc gttcccggcc tcgggcgcga
      181 tcgcgagcgc ccgctcgccc tgcgccgtga ggatcccgag gatctcctcg gagatcgcgt
      241 cgatcttcac ggacgaaagg gcaatgtggt actcgagcgc ctggagcccg cgcaggatcg
      301 gcaggatctc ggggtggtcc gcgacggccg tcgcgatgag gccgacgcgc gacgtgagcg
      361 ggcggttccg ccgcgcgacc tcgaggacgt gctcggccga gacgcgcacc tgcggcgcca
      421 tggcgtacgc cgcccagcag aagcggcaca tctccgagca gccgcgcgcg atctcgatca
      481 ggagcttgtc ggaaagctcc gtgtgcggcg tgaggatcgt cgtgtgcggc ggctcgcgga
      541 gcggcccctc gcccgccttg aactgctgga cgacgatctt tccctcgtcg gcgccctccg
      601 cgcgcgcgcc gtggaccgcg ggcacgtaga gccccttgac gtccgccagc gccgcgaccg
      661 tcccctcgcg ggagaggtcc cgccggagga tctccgcgat cgccgggacg atcttctcgc
      721 cgtcgcccat cgcgaaggcg tccacccact gcgcgagcgg cgccgggttg atgcgcgcgc
      781 agtcgccgcc gacgaggacg atcggatctc cctcgccgcg ctcggcgcgg tcgagcggga
      841 tgcgcgccgc gggcagcagg cggaggaagt tgacgtagtc catctcccac gagatcgaga
      901 aggcgacgag cgggaagtcg ccgagcggcg tgtccgtctc gagcgtgcgc gggccgcgca
      961 ggtcgccgtc gtcctccacg aagaaccggt cgcacgcgac gtcgtcctcc cggttgaaga
     1021 ggcggtagac ccactggaac ccgaggttcg acatcccgat ctcgtaggag ttcgggaagc
     1081 cgagcgcgac gcgggtgcgc ccgtggcggg acaggctctc cgtcgagcgc tcggcggcga
     1141 gcagcgtgcg gcggcggtca ggccggaatt gggtcactgt cctccgtgat cccctcgaac
     1201 agtatggtcg agaggtaccg ctcgccggta tccggcagca tcgcgaggag gaccgagccc
     1261 ttcggcgcct tctcggccac cttccgggcg gcggcgaacg tcgcgcccga ggacaggccg
     1321 cagaagatcc cttccttctg cgcgagctcg cgcgccgcgt cgcgggactc cacgtccgtc
     1381 acgaggaccg cctcgtcgta gaccttccgg ttgaagacgg ccggcacgaa gtccgtcgtc
     1441 cagccctgga tcttgtgggg cgcccacttg ccctccgtca ccatcggcgc ggcggcaggc
     1501 tccgtgaaca cgatcttgat gtcgggccgg gccgctttca ggatctcgcc cgcgccggtg
     1561 atcgtcccgc cggtgccgag gcccgagacg aagtagtcga gcctcttccc ggcgaagtcg
     1621 cgcaggatct cggggcccgt cgtgttccgg tggtactcgg ggttggcggg gttctcgaac
     1681 tgctggggca ggaaccagcc gtgcttgtcg gccagctccc tggcctgctt caccatcccg
     1741 gagccccggt tcgcggccgg gctcaggatc accttggcgc cgaggccctt catgagcttg
     1801 cggcgctcga ccgagaacga gtcggccatg atggcgacga acgggtagcc cttcacggcg
     1861 cagaccatcg cgagcgcgat tcccgtattg ccggacgtcg gctcgatcac ggtctgaccc
     1921 ggtttcagga cgccgcggcg ctccgcgtcc tcgatcatcg cgatcgcgag gcggtccttg
     1981 acggagctca tcgggttgaa ggcctcgcat ttcacgtaca tcgtcgtgcc ggcgggcgcg
     2041 aggcggttga tgcggacgac cggggtgttg ccgatggtgc cgaggatgct gtcgtggatc
     2101 acgtgcgacc tcccgtgggg gagcatcata aggaggggag cgcgacatga aatctagagt
     2161 ctcagtctca actatcgcgg ccggcgtcgt cctcgcggcc gtggcgttcg cttctcccgc
     2221 ccgggcccag ggcggcgtct ccgagtggcc ccgcgagttc acgacgccga agggcaacac
     2281 gatcgtcatg taccagcccc aggccgagac cttccagggc gaccgcgcga cgggccgcgc
     2341 ggcggtgtcg ttcctgaaga agggcgaggc ggcgccgaag ttcggcgtca tcttcttcag
     2401 cgcccgcgtc tccgtggacc gcgacacccg cctcgtctcc gtcctgggca tgaaggtgag
     2461 ccgcgtgcgg ttcccgggga tcacgccgga gggcgagaag aaattcacgg acaacctcga
     2521 ggccgaggtg ccgaagtggg acctcgtctt ctcctacgac cgcctcctcg agaacgtgaa
     2581 ggtcgcccag cgcgagaaga agagcgccga gggcctccgg cacgacccgc cgaagatcgt
     2641 cttcgccgac gagccgtccg tcctcctctc gtacgacggc gatcccgtct tccgcgaggt
     2701 cgagggcgcg cccgtgaagg tcgccgtgaa cacgtcgctc ctcgtcgtga acgacacgcg
     2761 gagcgggcgc tactacctct ccggcggcag gaagtggtgg tacgaggccg cgagcccgct
     2821 cggcccgtgg cgcacggtcg gcggcccgcc ggccgacatc gcggcgttcg ccgcacaggc
     2881 ccaggccgag cagaagaaag atgacgccga cacgaccggc gaggacggca cggaaggcgc
     2941 cgcccccccg cgcatcgtcg tcgcgacgga gcccaccgag atcgtcgtca cggagggcaa
     3001 gccggccctc aagccgatcg cgggcacgga cggcagcctg ctcgcggtgg acaacaccga
     3061 gggcgacgtc ctcttcgacg tgggtacgca ggactactac gtcctcctct cgggccgctg
     3121 gttcaaggga aagagcctcg catcgggcgg cgcgtggacg tacgtcgcgc ccgacgcgct
     3181 ccccgccgac ttcgcgaaga tcccgccgga atccgactcg ggcgacgtgc gcgccgccgt
     3241 cgcgggcacg gacgaggcgg aggacgcggt cctcgacgcc cagatcccgc agacgaccgc
     3301 cgtgaagcgc gcggaggcga acctccaggt ccagtgggac ggcgagccga agttcatcgc
     3361 gatcaccggc acggcgacgt cgtacgccgc gaacgcctcg acgtccgtgc tcctgatccg
     3421 cggccggtac tacgcgtgcg agaacgccgt gtggttcgtg tcggactcgg ccgaaggccc
     3481 gtggacggtc gccgactccg ttccgtccga cgacatcgac gcgattcccc cgagcgagcc
     3541 cgtctacaac gtcaagtacg tccgcatcta cgacgcgacg ccggacctcg tctatttcgg
     3601 ctacacgccc ggctacacgg gcatctaccc atggcacggc acggtcgtgt ggggaaccgg
     3661 ctggacttac cggccgtggg tcgggccgac gtactggtgg ccgcgcccgt acacgtgggg
     3721 cctgtgcgcc cgctacaacc cgtggacggg atggggcttc ggcttctcgt ggagctaccc
     3781 gttcttcagc gtgagcttcg gctggggcgg atggttccgg ccgcgcggct ggtacgggcc
     3841 ggcgtacggc agctggcatc ggcccggcgg ctggggctgg tacgggccgg gcggctaccg
     3901 cccgcccgcg gtcatcgcgg gccactactg gggcgcgcac ggcggcggca cgtggaaccg
     3961 gccgctcccg aacacgcggc ccggcgccgt gcgccccgcg gtgcgccccc ttcccggcgt
     4021 gcgcccgggc atcggtttcc gcccgccgac aaaggacatc cgtcctcccg cgcagtccaa
     4081 catctacaac cgcctcccgg ccaggtcgcg cgaggtcccg cggccgccgt cgacgttcga
     4141 gcggccgaag atctcgacgg gccggcccaa caacgtctac gccgacccga gcggagagat
     4201 ctaccgccgc acgaaggaag gacagtggca gcagcgcgag tccggacgct ggaagccgag
     4261 cagcggcggc gcgacggcga ggcccggggg cacgggcccc gcgacgcgtc cgccggccgg
     4321 ccagccgccc tcggtgacga cgaggc
//
LOCUS       DAOMFY010000316         4330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7433_length_4330_cov_7.106433, whole genome shotgun sequence.
ACCESSION   DAOMFY010000316 DAOMFY010000000
VERSION     DAOMFY010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7433_length_4330_cov_7.106433"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLB01_17635"
     CDS             complement(<1..231)
                     /locus_tag="PLB01_17635"
                     /inference="COORDINATES: protein motif:HMM:NF017809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HQQ79175.1"
                     /translation="MTACAELRVRAPFDGKALFGFFAARSLLGVERVEENSYERAFTQ
                     AGRAGTLRVALRRRGRSHSLSLEFPPWAKPDDL"
     gene            complement(228..854)
                     /locus_tag="PLB01_17640"
     CDS             complement(228..854)
                     /locus_tag="PLB01_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQQ79176.1"
                     /translation="MKTRADAWALLTEFTASQPLRRHALAVEATMRALARSTGVADPA
                     EIETWGLVGMLHDFDYEKFPTEQDHVWRGMEILRERGWPEPVIKAIGGHAFYTNIPRE
                     TPMEKAILAADELTGFCGACALIRPSKKIADVPVESVLKRLKEKSFARSVDRTYVTRG
                     AEEWGAPLPDLVALVLAAQVPIADRLGLGGAPAADLPDSPAPPEPPAP"
     gene            1133..2473
                     /gene="glnA"
                     /locus_tag="PLB01_17645"
     CDS             1133..2473
                     /gene="glnA"
                     /locus_tag="PLB01_17645"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HQQ79177.1"
                     /translation="MALKAAEILKICDKEGVRFLRLQFTDILGIIKNVEVPRTQFEKA
                     LEGEIQFDGSSIEGFTRIEESDMGLVPDLDTFRIFPWSHPNGKVARLVCDVITPDGKP
                     FAGCPRQTLKRQVEKAKAMGYTLMTGPEAEFFLFTRDANGDPVVDTHDQGGYFDLTPV
                     DRGEEARRDIVVALEAMGFEVEAAHHEVAPGQHEIDFKYGDAVTTADQVATFKLVVKK
                     VALDHGLHATFMPKPIFGVNGSGMHVHQSLITGSGASMKNAFYDPKAKYQLSGLAMNY
                     IGGILKHARAIAAITNPLVNSYKRLVPGYEAPVNVAWSERNRSPMVRIPARRGMGTRC
                     EVRMPDPSCNPYLAFTVMLAAGLDGVKNKLEAGEPVNQNIFEMSAREKKRLKIDVLPG
                     SLAEALDCLEKDDLLKDALGEHIYDKFVEAKRTEWQQYIAQVHGWEVDRYLQAY"
     gene            complement(2535..3227)
                     /locus_tag="PLB01_17650"
     CDS             complement(2535..3227)
                     /locus_tag="PLB01_17650"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HQQ79178.1"
                     /translation="MISHLPAAGLADRQGHFREVRRLSELETPRPAAAEGPWIALSRE
                     LGSGGGELARLLGEALGWRVYDREILSAVAEETHRETFALERFDEKGVREVGEYIAPL
                     ILHDDPGQARVLDGLRHVILRIGREGRAILVGRGANFLLAPAGGLRVRTVGPPAQRAE
                     ALARAEGIEVSEARRRVAESDAAQRAFVRQAFQHEIEDPAGYDLVLSPLNLGLPAAAA
                     AVLAAARTKLGL"
     gene            complement(3291..>4330)
                     /locus_tag="PLB01_17655"
     CDS             complement(3291..>4330)
                     /locus_tag="PLB01_17655"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQQ79179.1"
                     /translation="KAAGLDALVAVPSTSMAWLCGADPGRSERVMALVLRPKVPSAFV
                     TPFFEEERVRCDAVVGRTAVWQEHEDPVRLLAALLTGARRIGIEGTTDFHTASRLRDA
                     TGARLVDAAGLFDGLRAVKSDAEKAILRDAAARMVAAIAATHARLAAGMTEREVGEVL
                     SGELRRLGVRGGALVQFGASAALPHGGPGDARLEKGDVVLIDGGCRVRGYTSDITRTV
                     AFGSASDEVRKVYAAVDAAQRAAFAAFRAGAIPEDVDRSARKVIEDAGYGAFFTHRLG
                     HGLGMDGHEAPYLVQGNRVPLALGNVCTLEPGIYLPGRLGVRIEDDVFATADGCEALS
                     VRPSGLVVLPA"
BASE COUNT          707 a         1502 c         1492 g          629 t
ORIGIN      
        1 cagatcatcg ggtttcgccc aaggagggaa ttccagcgaa agtgaatgcg accgtccgcg
       61 gcgccggagc gcgactcgca gcgttccggc gcgccccgcc tgcgtgaatg ccctttcata
      121 ggaattctct tcgactctct cgactccgag cagcgagcgc gccgcgaaga atccgaacag
      181 cgccttgccg tcgaacggag cccgcacgcg cagctccgcg catgccgtca cggcgcggga
      241 ggctccggcg gcgcgggaga gtcaggcagg tccgccgcgg gcgcgccgcc gaggccgagg
      301 cggtccgcga tcggaacctg cgcggcgagg acgagggcca cgagatccgg gagcggcgcg
      361 ccccactcct cggcaccgcg cgtgacgtac gtgcggtcga cggagcgcgc gaacgacttc
      421 tccttgaggc gcttgaggac cgactcgacg ggcacgtccg cgatcttctt gctgggccgg
      481 atcagcgcgc acgcgccaca gaaacccgtc agctcgtcgg cggcaaggat cgccttctcc
      541 atcggcgtct cgcgcgggat gttcgtgtag aacgcgtggc cgccgatcgc cttgatgacg
      601 ggctcgggcc agccgcgctc gcgcaggatc tccatgccgc gccagacgtg gtcctgctcc
      661 gtggggaatt tctcgtagtc gaagtcgtgg agcatcccga cgaggcccca cgtctcgatc
      721 tcggcggggt ccgcgacgcc cgtcgatcgg gcgagcgcgc gcatcgtcgc ctcgacggcg
      781 agcgcatgcc ggcggagcgg ctgcgaagcg gtgaactcgg tcaggagggc ccaggcgtcc
      841 gcacgcgtct tcatgagcgg ggagtttaga gatcgggaga cgggggaacg ggagatttct
      901 tcgaaggtgc atggcggccc ggcgcgtccg gacctacgtt cgctgcgggg gcgctccggc
      961 gcgcggcggg ggctcgtatt caacttccga aaaattcgga ctgatcggta tgagatgcga
     1021 tttcatgcac cgaacggtgt gcaaatcgcc cgttttcgat tgactttctg gcgcattgaa
     1081 ctgattctcc gcccggcgcg agccgaaatc ggaaaaacgc agggggaacg agatggcact
     1141 taaggcagcc gaaatcctga agatttgcga caaggaaggg gtccgtttcc tgcgtctgca
     1201 gttcacggac atcctcggaa tcatcaagaa cgtcgaggtc ccgcgcacgc agttcgagaa
     1261 ggcgctcgag ggcgagatcc agttcgacgg ctcctcgatc gagggcttca cgcggatcga
     1321 ggagtcggac atgggcctcg tgcccgacct cgacaccttc cggatcttcc cgtggtcgca
     1381 ccccaacggg aaggtggcgc gcctcgtctg cgacgtcatc actcccgacg gcaagccctt
     1441 cgcgggctgc ccccgccaga cgctcaagcg ccaggtcgag aaggcgaagg cgatgggcta
     1501 cacgctcatg acggggccgg aagccgagtt cttcctcttc acgcgggacg cgaacggcga
     1561 cccggtcgtc gacacccacg accagggcgg ctacttcgac ctcactcccg tcgaccgggg
     1621 cgaggaagcc cgtcgcgaca tcgtcgtcgc cctcgaggcg atgggcttcg aggtcgaggc
     1681 ggcgcaccac gaggtcgccc ccggccagca cgagatcgac ttcaagtacg gcgacgccgt
     1741 gacgacggcc gaccaggtcg cgacgttcaa gctcgtcgtc aagaaggtgg ccctcgacca
     1801 cggcctccac gcgacgttca tgccgaagcc gatcttcggc gtcaacggct cggggatgca
     1861 cgttcaccag tcgctcatca cgggctcggg cgcgtccatg aagaacgcgt tctacgaccc
     1921 gaaggcgaag taccagctct ccggcctcgc gatgaactac atcggcggca tcctgaagca
     1981 cgcccgcgcc atcgccgcga tcacgaaccc gctcgtgaac tcgtacaagc gtctcgtgcc
     2041 cggctacgag gcgcccgtca acgtggcgtg gtcggagcgc aaccgttcgc cgatggtccg
     2101 catccccgcg cggcgcggga tggggacccg ctgcgaggtc cgcatgccgg acccgtcctg
     2161 caacccgtac ctcgcgttta cggtcatgct cgcggccggt ctcgatggcg tcaagaacaa
     2221 gctcgaggcg ggcgagcccg tgaaccagaa catcttcgag atgtcggccc gcgagaagaa
     2281 gcgcctgaag atcgacgtcc tcccgggcag cctcgccgag gcgctcgact gtctcgagaa
     2341 ggacgacctt ctcaaggatg cgctcggcga gcacatctac gacaagttcg tcgaggccaa
     2401 gcggaccgag tggcagcagt acatcgccca ggtccacggc tgggaagtgg accgctacct
     2461 gcaggcgtac tgattttctt gagggggaac cagggccggt tccccctcat accccctccg
     2521 agccaatcgt ctttctaaag cccgagcttc gtccgggccg ctgcgaggac ggcggcggcc
     2581 gcggcgggga gaccgagatt gagcgggctg agcacgaggt cgtaccccgc ggggtcctcg
     2641 atctcgtgct ggaaggcctg ccgcacgaaa gcgcgctggg cggcgtcgct ctccgcgacg
     2701 cgccggcggg cctcggaaac ctcgatgcct tccgcccggg ccagcgcttc ggcgcgctgg
     2761 gccggggggc cgacggtacg gaccctcaac ccgccggccg gggcgagaag gaaattcgct
     2821 ccccgcccca cgaggatcgc ccggccttcc cggccgattc gcaggatcac gtgcctcagc
     2881 ccgtccagca cgcgcgcctg gccgggatcg tcgtggagga tcaggggagc gatgtattca
     2941 cccacctcgc ggacgccttt ctcgtcgaag cgctccaagg cgaacgtctc gcggtgcgtc
     3001 tcctcggcga ccgccgagag gatctcgcgg tcgtagacgc gccagccgag cgcttcgccg
     3061 aggaggcggg cgagctcgcc tccccccgag ccgagctccc gcgaaagcgc gatccacggc
     3121 ccttccgccg cagccgggcg gggtgtctcc agctccgaga gccgacggac ctcgcggaaa
     3181 tggccctggc ggtcggcgag tccggcggcg ggaaggtggg aaatcatggc ggacctccct
     3241 tttccccaat ctagggcgcc atccggacgc gcgcatcccc tcgcggacgg tcaggcgggc
     3301 aggacgacga gcccggacgg gcggacggac agcgcctcgc atccgtccgc agtcgcgaag
     3361 acgtcgtctt cgatcctcac gccgagtctt ccgggtaggt agataccggg ctcgagcgtg
     3421 cagacgttgc cgagggcgag aggaacgcgg ttgccctgga ccagatacgg ggcctcgtgg
     3481 ccgtccatcc cgaggccgtg cccgagacgg tgcgtgaaga aagcgccgta gccggcatcc
     3541 tcgatcacct tgcgggcgga gcggtcgacg tcctcgggga tggcacccgc gcggaaggcg
     3601 gcgaacgccg cgcgctgggc ggcatccact gccgcgtaga ccttccggac ctcgtccgac
     3661 gcggagccaa aggccaccgt ccgcgtgatg tcggaggtat agccgcgcac gcggcagccg
     3721 ccgtcgatca ggacgacgtc gcccttctcg aggcgcgcgt cgcctgggcc cccgtgcggc
     3781 agcgcggcgg acgcgccgaa ctgcacgagt gcgccgccgc gcacgccgag gcggcggagc
     3841 tcgccggaca ggacctcgcc gacctcgcgc tccgtcatgc cggccgcgag ccgcgcatgc
     3901 gtggccgcga tcgcggcgac catgcgcgcc gcggcgtccc tcaggatcgc cttctccgcg
     3961 tcggacttga cggcgcgcag gccgtcgaac aggccggcgg cgtccacgag gcgggctccc
     4021 gtggcgtccc ggaggcgcga cgccgtgtgg aagtcggtcg tcccctcgat gccgatccgc
     4081 cgggcgccgg tgagaagcgc ggcgagcagc ctgaccgggt cttcgtgctc ctgccagacg
     4141 gccgtccgtc cgacgaccgc gtcacagcgg acgcgttctt cctcgaagaa cggcgtcacg
     4201 aacgccgagg gaaccttcgg tctcagaacg agcgccatga cccgttcgct ccgtcccggg
     4261 tcggcgccgc aaagccaggc catgctcgtc gacggcacgg cgacgagcgc atcgaggccg
     4321 gccgccttcg
//
LOCUS       DAOMFY010000317         4319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7459_length_4319_cov_7.922608, whole genome shotgun sequence.
ACCESSION   DAOMFY010000317 DAOMFY010000000
VERSION     DAOMFY010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7459_length_4319_cov_7.922608"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="PLB01_17660"
     CDS             <1..1605
                     /locus_tag="PLB01_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQQ79180.1"
                     /translation="LREAVASLAVASSTPLPGHAHVARVASAGWSGRGRTFVLGLDEA
                     RFPGGSAQDPVLLDHEREACNRRLGPNALPLRRGSAPEENRRALAALLARLRGRVVLS
                     YSNRDFLQDAEQFPSSAVLEAFRALEGKPRAGFRDLFEREGAPAAFRPSGEPLDEIEW
                     WLAALHAPGTDPARAAQEVEGAYPWIADGREAEDARRSPAFTKWDGRLTVFPGELDPR
                     ATREPISASRLQLLAKSPRGYFLKVVLGLDEPEEERPVDVWLDALEYGRLFHDTVFDF
                     FSERPDPDAPFELVRETARLQKVAERHLARMRGEIPPPTDVAFRTQRDAFLADLDVFV
                     KGEAKSAATSTPRFFEVPFGRSRGAAGKDLASAEPLEIPAGTSTFLLEGKIDRIDSEG
                     AGAWAVWDYKTGSERHFKESLPLNGGRQIQHALYARAAGILLARSGFRVDSLRSGYYF
                     PTRKGGGRRVLPEVTDAEVDATLTDLFDLLAAGAFPHSLEERDCEYCDYRMACGDPLD
                     AARRTKAKCAGDPILAPLAALEDRDV"
     gene            1598..4201
                     /locus_tag="PLB01_17665"
     CDS             1598..4201
                     /locus_tag="PLB01_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQQ79181.1"
                     /translation="MSDRLLKGGALPDAAARLRIETELDRNFLVEAGAGSGKTTSLVS
                     RMIALLASGAATPDSLVAVTFTRKAASQLRQKFQESLEERLRDEKDDAVRARLDRAQK
                     GLDLVRVGTIHSFCASLLRERPVEAGIDLGLSDVKLQEAALFQHVAWREWIAACSDED
                     DPRLAALEETGVRPQDLEEAFLRLAEYPDVEAVVASPPPQKPALDGVRRKTALLLEEA
                     APLKPEAPFGGKPADALQEALVRVRRLLNQPDAESDDGFVRVLEELEGEKLLKIKVTQ
                     WEGGKAAAQAFLARFEDFREKVLFPALLAWREWVHPRAMAFLLPALERLRTRRREEGF
                     LTYEDLLLISRDLLRDKPAVRRYFRSRVSHLLVDEFQDTDPLQAEIMLFLTAEDDRQK
                     NAWKLRPRPGSLFVVGDPKQSIYRFRRADIETYETFRDIVKASGGDVLPLTSSFRTVD
                     AVAGPVNATFEAILPKKASREQAAFAPLNARGAETGPGCGAFRIPSSGDGTMKENRVR
                     HQNAQDVAAFVRHALAGGWKIPGPGGAPVTPRPADILVLTAQRPALGDYAKALEKAGV
                     PVEVTGSKAFAITRGLVALQPLLAAVNDPDDEVAVVAFLSGPLCGVDDDALFRHRKAG
                     RRFAFVSDSDPGTDPKIARGLGLLREAHRDSRSLPPAAALVRIVERLGLAAWISAEEG
                     GRTATGNLLKVLAWARRLSADGHSFADVARRLVEEALELEEMSVDAARANAVRLMNIH
                     RAKGLEAPIVVLAEPVDRDEREPDAHVERTGEAPRGWFPILQSRGDRGVRVIGLPPDW
                     AEKSAAELAFLAAEQDRLFYVGATRAMYTLATSVHTKTTQKDGPRLTGLWARLDPHLA
                     PLPSRPVPVPA"
     gene            complement(4182..4289)
                     /locus_tag="PLB01_17670"
                     /pseudo
     CDS             complement(4182..4289)
                     /locus_tag="PLB01_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
BASE COUNT          587 a         1659 c         1489 g          584 t
ORIGIN      
        1 ctgcgcgagg ccgtggcgtc gctcgccgtc gcgtcctcca ctcctctgcc cgggcacgca
       61 cacgtcgcgc gcgtcgcgtc ggccggctgg agcggccgcg ggcgcacgtt cgtcctcggc
      121 ctcgacgagg cgcgcttccc cggcggcagc gcgcaggacc cggttctcct cgaccacgaa
      181 cgggaagcct gcaaccgtcg cctcggcccg aacgccctcc ccctgcgccg cgggtcggcc
      241 ccggaggaga accgccgcgc gctcgccgcg ctcctcgcgc ggctcagggg ccgcgtcgtc
      301 ctttcgtact cgaaccgcga tttcctccag gacgccgagc agttcccgtc ctccgccgtc
      361 ctcgaggcgt tccgggccct cgaagggaaa ccgcgcgccg gattccgcga tctcttcgag
      421 cgcgagggcg cgcccgccgc cttccggccg tccggcgagc cgctggacga gatcgagtgg
      481 tggctggccg cactgcacgc tcccgggacg gaccccgccc gggcggcgca ggaggtcgag
      541 ggcgcgtatc cctggatcgc ggacgggcgc gaggccgagg acgcgcggcg atcgcccgcc
      601 ttcacgaagt gggacggccg cctcacggtc ttccccggcg agctcgaccc ccgcgccacg
      661 cgcgagccca tttccgcctc gcgcctgcag ctcctcgcga agagtccgcg cgggtacttc
      721 ctgaaagtcg tcctcggcct cgacgagccc gaggaggaaa ggccggtgga cgtctggctc
      781 gacgcgctcg agtacggccg gctctttcac gacaccgtct tcgacttctt ctccgagcga
      841 cccgaccccg acgcgccctt cgagctcgtc cgcgagaccg cgcgccttca gaaggtggcc
      901 gagcgccacc tcgcgaggat gcgcggcgag atcccgccgc cgaccgacgt cgccttccgg
      961 acccagcgcg acgcgttcct cgcggacctc gacgtcttcg tgaagggcga ggcgaagtcg
     1021 gccgcgacgt ccacgccgcg cttcttcgag gtgccgttcg ggcgctcgcg cggcgccgcc
     1081 ggaaaggacc tcgcgtccgc cgagcccctc gagatcccgg caggaacctc gacgttcctc
     1141 ctcgagggaa agatcgaccg catcgactcg gagggtgccg gcgcgtgggc ggtctgggac
     1201 tacaagaccg gcagcgagcg ccatttcaag gagtcgctgc ccctgaacgg gggccggcag
     1261 atccagcacg cgctctacgc gcgcgcggcc gggatcctcc tcgcgcggag cggattccgc
     1321 gtggactccc tgcgctcggg ctattacttc ccgacgcgca agggcggcgg gcggcgcgtc
     1381 ctgcccgagg tcaccgacgc cgaggtcgac gccacgctga cggacctctt cgacctcctg
     1441 gccgcgggcg cgttccccca ctccctcgag gagcgcgatt gcgagtactg cgactaccgg
     1501 atggcctgcg gcgatccgct cgacgccgcg cgccggacga aggccaagtg cgcgggagac
     1561 ccgatcctcg cgccgctcgc cgcgctggag gaccgcgatg tctgaccggc tcctcaaggg
     1621 cggcgcgctc ccggacgcgg cggcgaggct ccgcatcgag acggagctcg accgcaactt
     1681 cctcgtcgag gccggcgcgg gctcggggaa gacgacgagc ctcgtctccc ggatgatcgc
     1741 gctcctcgct tcgggcgccg cgacgccgga ctccctcgtc gccgtgacgt tcacgcgcaa
     1801 ggcggcgtcg cagctgaggc agaagttcca ggagagcctc gaggagcgcc tccgggacga
     1861 gaaggacgac gcggtccgcg cccggctcga ccgcgcgcag aagggcctcg acctcgtgcg
     1921 ggtcgggacg atccactcct tctgcgcctc gctcctgcgc gagcgcccgg tcgaggcggg
     1981 catcgacctc ggcctctccg acgtgaagct gcaggaggcg gcgctcttcc agcacgtcgc
     2041 ctggcgcgag tggatcgccg catgctccga cgaggacgac ccacgcctcg ccgcgctcga
     2101 ggagacgggc gtccgcccgc aggatctcga ggaggcgttc ctgcgcctcg cggagtaccc
     2161 ggacgtcgag gccgtcgtcg cctccccgcc cccgcagaag cccgccctcg acggcgtgcg
     2221 gcgcaagacg gcgctccttc tcgaggaggc ggcgccgctg aagcccgagg cgccgttcgg
     2281 cggcaagccg gcggacgccc tccaggaagc gctcgtgcgc gtcaggcgcc tcctcaacca
     2341 gcccgacgcc gagagcgacg acggattcgt gcgcgtcctc gaggagctcg agggcgagaa
     2401 gctgctgaag atcaaggtca cccagtggga aggcggcaag gccgccgcgc aggccttcct
     2461 cgcccggttc gaggacttcc gcgagaaggt cctcttcccg gcgctcctcg cctggcgcga
     2521 atgggtccac ccgcgcgcga tggccttcct cctcccggcg ctcgagcgcc tgaggacgcg
     2581 ccggcgcgag gagggcttcc tcacgtacga ggacctcctg ctcatctcgc gcgacctcct
     2641 gcgcgacaag cccgccgtgc gccgctactt ccggagccgc gtgtcgcacc tcctcgtcga
     2701 cgagttccag gacaccgatc ccctgcaggc cgagatcatg ctgttcctga ccgccgagga
     2761 cgaccggcag aagaacgcgt ggaagctccg cccgcggccc gggtcgctct tcgtcgtcgg
     2821 cgaccccaaa cagtccatct accgcttccg gcgcgccgac atcgagacgt acgagacgtt
     2881 ccgggacatc gtgaaggcgt cgggcggcga cgtccttcct ctcacgtcga gcttccggac
     2941 ggtggacgcg gtcgccggcc ccgtcaacgc gacgttcgag gcgatcctcc cgaagaaggc
     3001 ctcgcgggag caggccgcgt tcgcgccgct gaacgcgcgc ggtgccgaga cgggcccggg
     3061 gtgcggagcc ttccggattc cctcgagcgg cgacgggacg atgaaggaga accgcgtccg
     3121 gcaccagaac gcgcaggacg tcgccgcgtt cgtccgccac gcgctcgcgg gaggctggaa
     3181 gatccccggc cccggcggcg cgcccgtgac gccgcgaccc gcggacatcc tcgtcctcac
     3241 cgcgcagcgc ccggcgctgg gcgactacgc gaaggcgctc gagaaggccg gcgtccccgt
     3301 ggaagtcacc ggcagcaaag ccttcgcgat cacgcgcggg ctcgtcgcgc tccagcctct
     3361 tctggccgcg gtgaacgacc ccgacgacga ggttgcggtc gtggcgttcc tctccggccc
     3421 actctgcggc gtcgacgacg acgccctctt ccggcaccgc aaggccgggc ggcgcttcgc
     3481 gttcgtttcg gactcggatc ccggcacgga ccccaagatc gcccgcggcc tcggcctcct
     3541 gcgcgaggcc caccgcgaca gccgctcgct tccccccgcc gcggccctcg tgcggatcgt
     3601 cgagcggctc ggcctcgccg cgtggatctc ggccgaggaa ggcgggcgga ccgcgaccgg
     3661 caacctcctg aaggtcctcg cgtgggcccg ccggctctcc gccgacggcc actccttcgc
     3721 ggacgtcgca cgccgcctcg tcgaggaggc gctcgagctc gaggagatga gcgtcgacgc
     3781 cgcgcgagcg aacgcggtcc gtctcatgaa catccacagg gccaagggtc tcgaggcgcc
     3841 gatcgtcgtc ctcgccgagc ccgtcgacag ggacgagcgc gagcccgacg cccacgtgga
     3901 gcgcacgggc gaggcgcccc gcggctggtt cccgatcctc cagtcgcggg gggaccgcgg
     3961 cgtccgcgtg atcgggcttc cgccggactg ggcggagaag agcgccgcgg agctcgcctt
     4021 cctcgccgcc gagcaggacc gcctcttcta cgtcggcgcg acgcgcgcga tgtacacgct
     4081 ggccacgagc gtccacacga agacgacgca gaaggacggg ccgcggctca cgggcctctg
     4141 ggcgcgcctc gacccgcacc tcgcgccgct cccctcgcgg ccggttcccg ttccggcgtg
     4201 agcggctgca ccaagagcag cttgatgccg gtgaggttct cgtccttgcg ggtcgagacc
     4261 accgtgccga tcacgcgggc aatctgcatt ccggcctagc ccttcttccc aaggttgag
//
LOCUS       DAOMFY010000318         4269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7611_length_4269_cov_7.038206, whole genome shotgun sequence.
ACCESSION   DAOMFY010000318 DAOMFY010000000
VERSION     DAOMFY010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7611_length_4269_cov_7.038206"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLB01_17675"
     CDS             complement(<1..475)
                     /locus_tag="PLB01_17675"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79182.1"
                     /translation="MRARAFGAVFGVALLVGATPRPSRAEDAVAYVLDATAAASSGRI
                     RVDIALPVPVAAPADLVMPRNYSGGYSLFLYDRFVEDVRAFSPAGSALAVRRAQNGPR
                     WTLGKSGETVARVAYDVDVPRMEREIWKGVATSKVRPRYAGFLGYSVFGYVDGDGD"
     gene            complement(472..1878)
                     /locus_tag="PLB01_17680"
     CDS             complement(472..1878)
                     /locus_tag="PLB01_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQQ79183.1"
                     /translation="MTLEESLFFLPASELGRRIKAKQLSPVALAEGYLDRLERIGPKL
                     GAVVTVTRDLALKEAKEAEAEIAAGKWRGPLHGVPYGAKDLLATKGIRTTWGAQPFRD
                     QVFDFDATVVTKLREAGAVLVAKLAMVELAGGFGYNNADASFTGPGRTPWNTDFWSGG
                     SSSGPGAATAAGLVAFSIGSETSGSIVTPCAFSGVTGLRPTYGRVSRHGAMALCWTLD
                     KLGPMCRSAEDCGLVMAAIAGKDPRDATSVSRPFRPAPPSARAKRPRIGVIKGSFEKT
                     QPEVRKNFEESLKVLGAFCDVVPDVEFPDYPYGAAVGTIVSAEGASAFRDLVENGRSK
                     ELRVPNDRWGGYAASMTLAVDYIQALRLRAPMKRALDALYAKFDALVSPARATVSYPI
                     GPDFDKVYPGVSGGPGVIGAGNLVGQPALVVPNGFGENGLPTAFQFTGRAFSDERLVA
                     LGTAVQARTEWHLKRPPV"
     gene            complement(1875..2237)
                     /locus_tag="PLB01_17685"
     CDS             complement(1875..2237)
                     /locus_tag="PLB01_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79184.1"
                     /translation="MPDRPTGTSRRAFARTLATMAAAPALLPTAALGQATAAPPAATP
                     TPTPVPTPEAPSSAAEALTEVVRIRWGKHLSGEQLGQIAKAIDGRLKAGEAMKKVTLG
                     NADEPDVVFSPAAEGSRP"
     gene            2556..>4269
                     /locus_tag="PLB01_17690"
     CDS             2556..>4269
                     /locus_tag="PLB01_17690"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HQQ79185.1"
                     /translation="MDPGRSPYREYDACGVGFVAHPRQSSHRVLRLALGGLSRVAHRG
                     ATGSDRTGDGAGILTRIPEPIFRRDAARRGIPLAEGAPVGVGTFFFPRGAEVRAAAIV
                     EKTFADEGLPLAGWRDVPVRTGRLGGWAETSRPSVVHALLTPPAGADADEWERRLYLA
                     GRVAARRARSEDLTGFWVVSLSHRTLVYKALLTGTELKAFYPDLEDPDYATSVAVFHQ
                     RYSTNTLPSWALAQPFHLLAHNGEINTLWGNRNAMRAREPELASPVWGDRMQRLRPVL
                     TDEGSDSTSLDEALELLVRSGRDPLHALTMLVPDAPQGHLPEALRAFYDFHAGLVEPW
                     DGPAAVAFTDGIVAGAHLDRSGLRPLRWKIADDGHVAAASEAGIVDLDHEHVVASGRL
                     GPGETIAVDTATGEILLDADVKSRLASRRPWGEWAGRHVRHVTPAPGPREALPEAELL
                     LRQAAHGWGVEDLRYVLAPSAAGQEPVFSMGDDAPIPPLSKSPQPLYAFFRQRFAQVT
                     NPPIDSLRETAVMSLRMDLGRGGSLLLEEPGDAHFLSLEHPVLLEDDAADHPGQVVAE
                     TDVGA"
BASE COUNT          578 a         1545 c         1554 g          592 t
ORIGIN      
        1 ggtcgccgtc gccgtccacg tatccgaaga cggagtaccc gaggaagccc gcgtaccgcg
       61 gccggacctt cgacgtcgcg acgcccttcc agatctcgcg ctccatgcgg gggacgtcga
      121 cgtcgtaggc gacccgggcg acggtttcgc cgctctttcc gagagtccag cgcggcccgt
      181 tctgcgcgcg ccggaccgcg agcgcagagc ccgcgggcga gaaagccctc acgtcctcga
      241 cgaaccggtc gtacaggaag agggagtagc cgccggagta gttgcgcggc atgacgagat
      301 cggccggggc cgcgacggga acggggagcg cgatatcgac gcggatccgc cccgaggagg
      361 ccgcggccgt cgcgtcgagg acgtacgcga cagcgtcttc cgccctcgag ggacggggag
      421 tcgcgccgac gagcagcgcg acgccgaaga cggcgccgaa ggcgcgggcc ctcaaaccgg
      481 cggcctcttg aggtgccact ccgtccgcgc ctgcacggcg gtcccgagcg cgacgagcct
      541 ctcgtcggag aacgcccgcc ccgtgaactg gaacgcggtc gggaggccgt tctcgccgaa
      601 gccgttcggc acgacgagcg cgggctggcc gacgaggttg cccgcgccga tcacgcccgg
      661 cccgccgctg acgcccgggt agaccttgtc gaagtccggg ccgatcgggt acgagaccgt
      721 cgcgcgcgcg ggcgagacga gcgcgtcgaa cttcgcgtag agcgcgtcga gcgccctctt
      781 catcggcgca cggaggcgca gcgcctggat gtaatcgacc gcgagcgtca tcgaggcggc
      841 gtagcccccc caccggtcgt tcgggaccct aagctccttc gagcggccgt tctccacgag
      901 gtcgcggaat gcgctcgcgc cctcggccga cacgatcgtc ccgaccgcgg cgccgtacgg
      961 gtagtccggg aactcgacgt cggggacgac gtcgcagaac gcgccgagca ccttcagtga
     1021 ctcctcgaag ttcttccgga cctcgggctg cgtcttctcg aacgagccct tgatgacgcc
     1081 gatccgcggg cgcttcgccc tcgcggacgg cggcgcgggg cggaacgggc ggctcacgga
     1141 ggtcgcgtcc ctcggatcct tgcccgcgat cgcggccatc acgaggccgc agtcctcggc
     1201 gctccggcac atcgggccga gcttgtcgag cgtccagcag agcgccatcg cgccgtgacg
     1261 gctcacgcgt ccgtacgtcg gccggaggcc cgtgacgccg gagaacgcgc agggcgtcac
     1321 gatggagccg gacgtctcgg agccgatcga gaacgcgacg agcccggccg ccgtggcggc
     1381 gccggggccc gaggacgagc cgccggacca gaagtccgtg ttccacggcg tgcggcccgg
     1441 acccgtgaag gacgcgtccg cgttgttgta gccgaacccg cccgcgagct cgaccatcgc
     1501 gagcttcgcg acgaggacgg cgccggcctc gcggagcttc gtcacgaccg tcgcatcgaa
     1561 atcgaagacc tggtcgcgga acggctgggc gccccacgtc gtccggatgc ccttcgtcgc
     1621 gaggaggtcc ttcgcgccgt acgggacgcc gtggagcgga ccgcgccact tcccggccgc
     1681 gatctcggcc tcggcctcct tcgcttcttt cagggcgagg tcgcgcgtga cggtcacgac
     1741 ggcgccgagc ttcggcccga tgcgctcgag ccggtcgagg tatccctcgg cgagcgcgac
     1801 cggcgagagc tgctttgcct tgatacggcg gcccagctcg gaggccggga ggaagaagag
     1861 agattcttcg agagtcatgg acggcttcct tcggcggcgg gggagaagac gacgtccggc
     1921 tcgtcggcgt ttccgagcgt caccttcttc atcgcctctc ccgctttcag cctcccgtcg
     1981 atggccttcg cgatctgccc gagctgctcg ccggagagat gcttgcccca ccggattcgc
     2041 acgacctccg tgagcgcctc ggccgcggag gagggcgcct cgggcgtcgg gacgggggtc
     2101 ggcgtcggcg tcgccgcggg tggagcggct gtcgcctggc cgagcgcggc ggtcgggagg
     2161 agggcgggtg ccgcggccat cgtcgcgagc gttcgcgcga acgctcgacg cgaggtcccg
     2221 gtggggcggt cgggcatttt cttcgcctcc ttcgtcggaa tttccgtctt cgaatctatc
     2281 ttgattcggc gcgtcccgct ccccatactc cgcgcgtcat gacgtccctc gccgtcgtcc
     2341 gcatcgcgtc gaccctcgtc ggggatgggt ccattcgcct cgtcctcgtg aagcgcacgc
     2401 ggaggggcgg gcgaggggac taaccgggaa ggccaacgga accccccaag cccgctcatc
     2461 cgagcgggct tttttctttt cggggcacta agaagaagga aggagccggg atgccggagg
     2521 gcccagggag acgagagcga cgctggacgg acggggtgga ccccggccgg agcccgtacc
     2581 gcgagtacga cgcctgcggc gtggggttcg tggcccaccc gcgccagtct tcgcaccgcg
     2641 tcctgcgcct cgcgctcggc ggcctctccc gcgtcgccca caggggcgca accggcagcg
     2701 accggacggg cgacggcgcc ggcatcctga cgcgcatccc cgagccgatc ttccggcgcg
     2761 acgccgcgcg gcgcgggatt cccctggcgg agggcgctcc ggtcggcgtc gggaccttct
     2821 tcttcccccg gggcgccgag gtgagggcgg cggcgatcgt cgagaagacg ttcgcggacg
     2881 aggggctgcc gctcgcaggc tggcgcgacg tgcccgtgcg gacgggccgc ctcggaggct
     2941 gggccgaaac gtcgcggccg tccgtcgtgc acgcgctcct gacgccgccg gcgggcgccg
     3001 acgcggacga gtgggagcgc cgcctctacc tcgcggggcg cgtcgcggcg cgccgggcga
     3061 ggagcgaaga cctcaccgga ttctgggtcg tgtcgctctc gcaccggact ctcgtctaca
     3121 aggcgctcct caccgggacg gagctcaagg cgttctaccc ggatctcgag gaccccgact
     3181 acgcgacgag cgtcgccgtc ttccaccagc ggtattcgac gaacacgctc ccgagctggg
     3241 cgctcgcgca gccgttccac ctgctcgcgc acaacggcga gatcaacacg ctctggggca
     3301 accgcaacgc gatgcgggcg cgcgagccgg agctcgcgtc gcccgtgtgg ggcgaccgga
     3361 tgcagcgcct gcgcccggtc ctcacggacg aggggagcga ctcgacgagc ctcgacgagg
     3421 cgctcgaact cctcgtgcgg tcggggcgcg acccgcttca cgcgctcacg atgctcgtcc
     3481 ccgacgcgcc gcagggccac ctgcccgagg cgctccgcgc gttctacgac ttccacgcgg
     3541 gcctggtcga gccgtgggac ggcccggcgg ccgtcgcgtt cacggacggg atcgtcgcgg
     3601 gcgcccacct cgaccgctcc gggctccggc ccctgcgctg gaagatcgcg gacgacgggc
     3661 acgtcgccgc cgcgtccgag gcggggatcg tggacctcga ccacgagcac gtcgtcgcga
     3721 gcggccgcct cggccccggc gagacgatcg cggtggacac ggcgacgggc gagatcctcc
     3781 tcgacgcgga cgtgaagtcg cgtctcgcct cgcgccgccc gtggggggag tgggcggggc
     3841 gccacgtccg gcacgtgacc ccggctccgg gcccccgcga ggcgctgccg gaagccgaac
     3901 tcctgctccg gcaggcggcg cacggctggg gcgtggagga tctccgctac gtcctcgcgc
     3961 cgtcggcggc cgggcaggag cccgtgttct cgatgggcga cgacgcgccg atccctccgc
     4021 tctcgaagtc gccccagccg ctctacgcgt tcttccgcca gcgcttcgcg caggtgacga
     4081 acccgccgat cgactcgctt cgcgagacgg cggtcatgtc gctgcggatg gacctcggcc
     4141 gcggcggctc gctcctcctc gaggagccgg gcgacgcgca cttcctgagc ctcgagcatc
     4201 ccgtcctcct cgaggacgac gcggccgatc atccaggtca ggtggttgcc gagacagacg
     4261 tgggggccg
//
LOCUS       DAOMFY010000319         4225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7751_length_4225_cov_7.675540, whole genome shotgun sequence.
ACCESSION   DAOMFY010000319 DAOMFY010000000
VERSION     DAOMFY010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7751_length_4225_cov_7.675540"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLB01_17695"
     CDS             <1..836
                     /locus_tag="PLB01_17695"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQQ79186.1"
                     /translation="AGDVGARRYFRQSAHGGGRVLVVLYPQPESTAQANWAAIGEALA
                     RVGVRVPALLDDAPELGAALIEDLGDRDLSMDLADAPREDRPRLLDEAEELLTPIRTI
                     ARAAATRNPAFDAAFFAAELDHTRHWALEKDGQDPLSPARAAEWARLAGALAAAAADP
                     AATGDAVPTHRDYHANNLMRTAEGRLAPIDFQDLRLGPPDYDSASLRFERAGASVEAD
                     AGTVSEAVLLQRAWKVLGTFEKMLLKGRPFYRPHRETARRVIRRHTTPGGPFEGLLAF
                     L"
     gene            877..1062
                     /gene="tatA"
                     /locus_tag="PLB01_17700"
     CDS             877..1062
                     /gene="tatA"
                     /locus_tag="PLB01_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQQ79187.1"
                     /translation="MPNLGLPELLVILVIVILVFGAGKIPQLGKGLGEGIKNFKDAMR
                     QGESGDDKKDDGASKPK"
     gene            1123..1971
                     /locus_tag="PLB01_17705"
     CDS             1123..1971
                     /locus_tag="PLB01_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HQQ79188.1"
                     /translation="MNTLKIVFLMTFLTVLLVAVGQATFGSRGAVTALVIAAVMNLFT
                     YFFSDRMVLAAYGARVVTEEEAPELTAVVRSLAAKANVPMPRVAVIPQDAPNAFATGR
                     DPQHAVVAATEGILKILNRDELEAVLSHELGHVTNRDILLGAIAATLAGALTHLAQMA
                     MWTGGGRRDDREGGGGLLGILAIVFAPIAAMIVQAMISRQAEFRADETGAALSGRPLE
                     LASALRKLEAASQRIPMDGSPATAHLFIVNPFGGGLARLFSTHPPTEERVARLEAMAR
                     GGLRPS"
     gene            complement(1968..2207)
                     /locus_tag="PLB01_17710"
     CDS             complement(1968..2207)
                     /locus_tag="PLB01_17710"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQQ79189.1"
                     /translation="MSVTIDGQLQRIIDELVSKGIPLEAAKREFEKKYVSAAVMRASG
                     NMGRAAKSLGIHRNTLRAKISLLKVKPPLRGRDNS"
     gene            complement(2314..3177)
                     /locus_tag="PLB01_17715"
     CDS             complement(2314..3177)
                     /locus_tag="PLB01_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79190.1"
                     /translation="MRHRLAALSAGVLVAVSLAARADDAPKAPVLAREGAAAPAVPQS
                     RLEKVARPPAPITGPLPIGVEVDVYCSGYLGDRDEEFVGRIVNAEKERNQSMFMAGDV
                     LYIDIGAENGVQAGMEFTIVRPSRLVNRWGSEREVAGRIYMTPGRVRVLCAQEKSSIA
                     ELIYSCNEVEVGDFVAPFEPLPVPLVRRTRPMTICDTPNGKPIGHVVDTRDAVTPVGT
                     DTIVFLDLGEQDGLNPGDFLTVYHPSTQAEGVRTVLGEAAILTTRARSSVAIITSMVD
                     FIAIGDAVEVK"
     gene            complement(3301..4143)
                     /gene="bamD"
                     /locus_tag="PLB01_17720"
     CDS             complement(3301..4143)
                     /gene="bamD"
                     /locus_tag="PLB01_17720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQQ79191.1"
                     /translation="MHSPTSVTPLPVLPRRLFGPIALAALLGGAAGCASTDSAAKMTR
                     ELLSMPKEEAYAKGESLVQKKKYELGRQYLRFVAENYANDAIGKQAALRLADSFFDEK
                     TPLGYLEAGVRYKDFRSRYPSHPRSDYALFRLAQCSDKQAESPDREQTNTRTAALAYR
                     ELVLAYPDSPYATEGRLRLAAMRNLLAEHEYVVGHFYVKRRAWRAAKGRMDGVLAAYP
                     DYAKLDKVLYDNGLAENKMGHEDEARQLWDRLARDFPSSPLLKKVPRPAAPPPAPPAK
                     AAGG"
BASE COUNT          627 a         1498 c         1460 g          640 t
ORIGIN      
        1 tggccggcga cgtcggcgcg cgccgctact tccgccagag cgcgcacggg ggcggccgcg
       61 tcctcgtcgt cctgtacccg cagcccgaat ccacggcgca ggcgaactgg gccgcaatcg
      121 gcgaggccct cgcgcgcgtg ggcgtgcggg ttcccgcact cctcgacgac gcgcccgagc
      181 tcggcgccgc cctcatcgag gacctcggcg accgcgacct ctcgatggac ctcgcggacg
      241 caccgcgcga ggaccggccc cgcctcctcg acgaggccga ggagctcctc acgccgatcc
      301 gcacgatcgc acgcgccgcc gcgacgcgaa atcccgcgtt cgatgcggcc ttcttcgccg
      361 ccgagctcga ccacacgcgc cactgggccc tcgagaagga cggacaggat cccctctccc
      421 ccgcgcgcgc cgccgagtgg gcccgcctcg cgggcgcgct cgcggccgca gccgccgatc
      481 ccgccgcgac gggcgacgcc gttccgacgc accgcgacta ccacgcgaac aacctcatgc
      541 gcaccgccga agggcgcctg gccccgatcg acttccagga cctgcgcctc gggccccccg
      601 attacgactc ggcttcgctc cgcttcgagc gcgccggcgc gtccgtcgaa gccgacgccg
      661 ggaccgtgtc ggaggccgtc ctcctgcagc gggcgtggaa ggtcctcggg acgtttgaga
      721 agatgctcct caagggccgc ccgttctacc gtccgcaccg cgagacggcg cgccgggtca
      781 tccgccgcca caccactccc ggagggccct tcgaggggct cctcgcgttt ctctgatcac
      841 ggcccggagt agaatccgtc ccttcaggag gcacccgtgc cgaatctcgg actccccgaa
      901 ctgctcgtca tcctcgtgat cgtgattctc gtgttcggcg cggggaagat cccgcagctc
      961 ggcaagggcc tcggcgaggg catcaagaac ttcaaggacg cgatgcgcca gggcgagtcc
     1021 ggcgacgaca agaaggacga cggcgcctcc aagccgaaat aaagcggcgc cccccttcgt
     1081 ttcgttccgg gacggcgcac ccgccggcgc ccggagacac cgatgaacac gctgaagatc
     1141 gtcttcctca tgacgttttt gaccgtcctg ctcgtcgcgg tcgggcaggc cacgttcggc
     1201 tcgcggggag ccgtgacggc cctcgtgatc gcggccgtca tgaacctctt cacgtacttc
     1261 ttctcggacc ggatggtcct cgcggcctac ggcgcgcgcg tcgtgacgga agaggaggcg
     1321 ccggagctca ccgcggtcgt ccggtcgctc gcggcgaagg cgaacgtccc gatgccgcgc
     1381 gtggcggtca tcccgcagga cgcgccgaac gccttcgcga ccggccgcga cccgcagcac
     1441 gccgtcgtcg cggccaccga gggcatcctg aagatcctga accgtgacga gctggaagcc
     1501 gtcctctccc acgagctcgg ccacgtgacg aaccgcgaca tccttctcgg cgcgatcgcg
     1561 gcgacgctcg ccggcgcgct cacccacctc gcgcagatgg cgatgtggac gggcggcggc
     1621 cggcgggacg accgcgaagg cggcggcgga ctcctgggaa tcctcgcgat cgtcttcgcc
     1681 ccgattgcgg ccatgatcgt ccaggccatg atctcccgcc aggcggagtt ccgggcggac
     1741 gagacgggcg cggcgctctc ggggcggccg ctcgaactcg cctcggcgct ccggaaactc
     1801 gaggcggcct cgcagcggat cccgatggac ggctcaccgg ccacggcgca cctgttcatc
     1861 gtgaacccgt tcgggggagg cctcgcgcgt ctcttctcga cgcacccgcc caccgaggaa
     1921 cgggtcgcgc gcctcgaggc catggcgcgc gggggtctca gaccctccta ggaattgtcc
     1981 cggccccgga gcggcggctt gaccttcagg agggagatct tcgcccgcag ggtgttgcgg
     2041 tggatgccga gagacttggc ggcgcggccc atgttgccgg acgcgcgcat gacggccgcg
     2101 gaaacatact tcttctcgaa ttccctcttg gccgcctcga gcggaattcc cttcgacacg
     2161 agctcgtcga tgatgcgctg cagctggccg tcgatcgtca cagacacgtt cctcccgcac
     2221 acttgcaagc catgaggctg cttgggggaa acggaaatta tcacgggatc ttcgtcccgc
     2281 aagtcttttc tgagcgggcc gaaaaagaag ggcttacttg acttcgacgg cgtcgccgat
     2341 ggcgatgaaa tcgaccatcg acgtgatgat cgcgaccgac gaccgtgcgc gcgtcgtgag
     2401 gatcgccgcc tcgcccagca ccgtacgcac gccctcggcc tgcgtcgacg ggtggtacac
     2461 cgtgaggaag tcgcccgggt tcagtccgtc ctgttccccg aggtcgagga agacgatcgt
     2521 gtccgtcccg acgggcgtca cggcgtcccg cgtgtcgacg acgtggccga tcggcttgcc
     2581 gttcggcgtg tcgcagatcg tcatgggccg cgtgcggcgc acgagcggca ccggaagcgg
     2641 ttcgaacggc gcgacgaaat ccccgacctc gacctcgttg caggagtaga tgagctcggc
     2701 gatcgaggac ttctcctggg cgcagagcac gcggacgcgc ccgggcgtca tgtagatgcg
     2761 gcccgcgacc tcgcgctcgg agccccagcg attcacgagg cggctcgggc gcacgatcgt
     2821 gaactccatc ccggcctgga cgccgttctc ggccccgatg tcgatgtaga ggacgtcgcc
     2881 ggccatgaac atcgactggt tgcgttcctt ctcggcgttc acgatgcgcc cgacgaactc
     2941 ctcgtcgcgg tccccgaggt agcctgagca gtagacgtcc acctcgacgc cgatgggcag
     3001 cggtccggtg atcggggcgg ggggccgcgc gaccttctcg aggcgcgact ggggcacggc
     3061 gggagcggcg gcgccctcgc gtgccaggac gggcgccttc ggcgcgtcgt cggcgcgggc
     3121 ggcgagcgag acggcgacca ggacgccggc cgagagcgct gcgagacgat gacgcatgac
     3181 gacctcctgc ccgaaacccg tgtccccgaa ccgaccatgg gggcgcgaaa aggggacgaa
     3241 tggacggcgt atcttaattc gctggagccg cttgcgtcaa cccgagaggc tgacgcgggc
     3301 tcagccgccg gcggccttcg cgggaggagc gggcggcggc gccgccggac gcggcacctt
     3361 cttcaggagc gggctggagg gaaagtcgcg cgcgaggcgg tcccagagct gccgggcctc
     3421 gtcctcgtgt cccatcttgt tctcggcgag tccgttgtcg taaagcacct tgtcgagctt
     3481 cgcgtaatcc ggatacgccg cgaggacgcc gtccatgcgg cccttggccg cgcgccacgc
     3541 ccggcgcttc acgtagaagt gtccgacgac gtactcgtgc tcggcgagaa ggttgcgcat
     3601 ggccgcgagg cgcaggcggc cttccgtggc gtagggcgaa tccgggtacg cgaggacgag
     3661 ctcgcggtac gcgagagccg cggttcgcgt gttcgtctgc tcgcggtccg ggctttcggc
     3721 ctgcttgtcc gagcactggg cgagacggaa gagcgcgtag tccgagcgcg ggtgcgacgg
     3781 gtagcggctc cggaagtcct tgtacctcac gccggcctcg aggtagccga gcggcgtctt
     3841 ctcgtcgaag aacgagtccg cgagccgcag ggcggcctgc ttgccgatcg catcgttcgc
     3901 gtagttctcg gcgacgaacc gcaggtactg ccgcccgagc tcgtacttct tcttctggac
     3961 gagagattcg cctttcgcgt aggcctcctc tttcggcatg gagaggagct cccgcgtcat
     4021 cttcgccgcc gagtcggtgc tcgcgcagcc cgccgctccc ccgaggaggg cggcgagggc
     4081 gatcggcccg aagaggcggc gcgggaggac cggaaggggc gtcacggacg tcggagaatg
     4141 cacggcggcg gggattcttt caagcgccct gccgcacgcc ggccgcccgc gcagccgtaa
     4201 ggctacggcc ggcgcctgat ctcga
//
LOCUS       DAOMFY010000320         4218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7782_length_4218_cov_6.807591, whole genome shotgun sequence.
ACCESSION   DAOMFY010000320 DAOMFY010000000
VERSION     DAOMFY010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7782_length_4218_cov_6.807591"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PLB01_17725"
     CDS             complement(<1..163)
                     /locus_tag="PLB01_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HQQ79192.1"
                     /translation="MPTNVIMPQMGESIAEGTLTKWLKKAGDSVKRDEPLFEISTDKV
                     DAEIPAPASG"
     gene            complement(179..1213)
                     /locus_tag="PLB01_17730"
     CDS             complement(179..1213)
                     /locus_tag="PLB01_17730"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HQQ79193.1"
                     /translation="MADPRVARGGVTYTLTPDGSGVETTYLDAIRQALFEEMERDERV
                     FVLGEDVGVYGGAFKATEGLLERFGPARVLDTPLAEEAIVGAAIGAAMRGLRPVAEMQ
                     FIDFISRAFDLLTNFAAKNRYRTGIGVPLVVRGPSGAGVHGGPYHSQSPEMYFVKTPG
                     LKVVAPATAADARALLKASIRDDDPVIFLEHKFLYRRVRDRLVPGEDAGTLGKAAVRR
                     PGTDLTIVTYGAMLWTALEAAPALEVDGISPEILDLRTLMPWDEDAVLTSVKKTGKCL
                     ILHEDTRTAGIGAEVSATITEKAFEWLDAPILRVTAPDTPVPYSPPLEEAFLPNTKTL
                     LAAARQLARY"
     gene            complement(1213..2229)
                     /locus_tag="PLB01_17735"
     CDS             complement(1213..2229)
                     /locus_tag="PLB01_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HQQ79194.1"
                     /translation="MTTSLPPERLKDLYRTMLLNRLLEERLVNLYRQGKVVGGLYRSL
                     GQEATSVATAFALEPGDLLAPLIRNLGSVLARGIPARDVLTQYLARATSPSHGKDANL
                     HFSVPDKGVYSPIAMLGTLVPVVSGMLLAERMKGNSVVGMTYLGDGGTSVGAFHEGIN
                     FAAVQKLPLVLVVEFNGWAYSTPFEKQSAAKTLADKAIGYGIRGEVVDGNDAIAVYEV
                     TRAAVERARSGGGPTLLECKTYRMKGHAEHDAQAYVDPKELAEWREKDPLPRLARVLV
                     ESGTASAADLRAAHEEIGRALDADVAFAEASPFPDPSLAFRGVWADEALGERARAGVF
                     TGGL"
     gene            2402..2929
                     /locus_tag="PLB01_17740"
     CDS             2402..2929
                     /locus_tag="PLB01_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79195.1"
                     /translation="MKTLVRVSMMAVLAAPALARAAGTLTGVTASPAPAVAGSEVTIT
                     VTSTGPCAVYVDFGDKQKAAHLSLPSPVKHVYAAPGKYVIRTFAYTSGNEAPGLSRCG
                     GFADMELVVKAAAPVIAPNRNFVPPAGASSGVSKVTNPAPSGGGATAEPSGAARTLGG
                     KVAPTPTATPTKMVR"
     gene            2958..3959
                     /locus_tag="PLB01_17745"
     CDS             2958..3959
                     /locus_tag="PLB01_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79196.1"
                     /translation="MRGRPRIAARVCALGVALLLPRPLYGLPVSWDVQAKGVFTYRVG
                     SEGGVGKRGQETTTFLEGEDVQIECLYLVKQGGDKKDFTTWQLGLSVDGSGMHSWPMN
                     ANVGTVGGVGSKSYVWKADKSGSRKIRCAAETYDADPSNNAAEITVTVKARPPLTEME
                     IPTPVPQSPPPGKKIALVPNLYLTLSAQVPQRPVGGWPNLFDYVIHRPYQSVKWHVDI
                     VGPSRGGAISLSTRPAGDFQGFVSNLSYKDFNAFVTRDWLDARGLGPGHYAALFYLSE
                     TANGKTTNGKKGVVEFELVAPLMAGAQPAGGGAAAGPSDAMPTSSGKAPTPTPARRK"
BASE COUNT          655 a         1409 c         1512 g          642 t
ORIGIN      
        1 tgccggacgc gggggcgggg atctcggcgt cgaccttgtc ggtcgagatc tcgaacaggg
       61 gctcgtcgcg cttgaccgag tcgccggctt tcttgagcca cttcgtgagc gttccctcgg
      121 cgatcgactc gcccatctgc ggcatgatca cgttcgtcgg catgttggtc tcctcgagtc
      181 agtagcgcgc cagctgccgc gcggccgcca gcagggtctt cgtgttgggg aggaacgcct
      241 cctccagggg tggagagtac ggcaccggcg tgtcgggcgc cgtgacgcgc aggatcgggg
      301 cgtccagcca ctcgaacgcc ttttccgtga tcgtcgcgga gacctcggcg ccgattcccg
      361 cggtgcgcgt gtcctcgtgg aggatcaggc acttgccggt cttcttcacg gaggtcagga
      421 cggcgtcctc gtcccacggc atgagcgtcc ggagatcgag aatttcgggc gagattccgt
      481 ccacctcgag cgcgggcgcg gcctcgagcg cggtccagag catcgcgccg tacgtcacga
      541 tcgtgaggtc cgtcccgggg cgccggacgg cggccttccc gagtgttccg gcgtcctcgc
      601 cggggaccag ccggtcgcgc acgcgccggt agaggaactt gtgctcgagg aagatgacgg
      661 ggtcgtcgtc gcggatggag gccttcagga gtgcgcgcgc gtccgcggcc gtcgccggag
      721 cgacgacctt gaggcccggg gtcttcacga agtacatctc gggcgactgc gagtgatacg
      781 gaccgccgtg gacgccggcg cccgaggggc cgcggacgac gagcgggacg ccgatgcccg
      841 tgcggtaccg gttcttggcc gcgaagttcg tcaggaggtc gaacgcgcgc gagatgaagt
      901 cgatgaactg catctcggcg acggggcgca gcccgcgcat cgcggcgccg atcgcggcgc
      961 cgacgatcgc ctcctcggcg agcggcgtgt cgagaacgcg cgcggggccg aagcgctcga
     1021 ggagcccctc ggtcgccttg aacgcgccgc cgtagacccc tacgtcctcg ccgaggacga
     1081 agacgcgctc gtcgcgctcc atctcctcga agagagcctg ccggatcgcg tcgagatacg
     1141 tcgtctcgac gccggaaccg tccggagtga gcgtgtacgt gaccccgccc cgggcgacgc
     1201 gcgggtcggc catcagaggc ctcccgtgaa gacgcccgcg cgtgcccgct cgccgagggc
     1261 ctcgtcggcc cagacgccac ggaaggcgag cgacggatcg gggaagggcg acgcctccgc
     1321 gaacgcgacg tccgcgtcga gcgcacgccc gatctcctcg tgcgcggccc gcaggtccgc
     1381 ggcggacgcg gttccggact cgacgaggac acgcgcgagc cggggaagcg ggtccttctc
     1441 ccgccactcg gctagctcct tcgggtcgac gtaggcctgc gcgtcatgct cggcgtggcc
     1501 cttcatccgg tacgtcttgc actcgaggag cgtcggcccg ccgccggagc gggcgcgctc
     1561 gacggccgcg cgcgtgacct cgtacaccgc gatcgcgtcg ttgccgtcca cgacctcgcc
     1621 ccggatgccg tagccgatcg ccttgtccgc gagcgtcttc gccgcgcttt gcttctcgaa
     1681 cggcgtcgag tacgcccagc cgttgaactc gacgacgagg acgagcggga gcttctggac
     1741 cgcggcgaag ttgatgccct cgtggaacgc gcccacggac gtcccgccgt cgccgaggta
     1801 cgtcatcccg acgacggagt tccctttcat ccgctcggcg aggagcatcc ccgacacgac
     1861 gggcacgagc gtcccgagca tcgcgatcgg ggagtagacg cccttgtccg gcaccgagaa
     1921 gtgcaggttc gcgtccttgc cgtgcgaggg cgaggtcgcg cgggccaggt actgcgtgag
     1981 gacgtcgcgg gcggggatcc cgcgcgcgag gaccgagccc aggttgcgga tgagaggggc
     2041 gaggaggtcg ccgggttcga gggcgaacgc ggtcgcgacg gacgtcgcct cctgcccgag
     2101 cgagcggtac agccccccga cgaccttgcc ttgccggtac aggttcacca gccgctcctc
     2161 gagcaggcgg ttgagcagca tggtgcggta gaggtccttc aggcgttcgg gggggaggct
     2221 tgtcgtcatg tcttggggtc gcccgggagc gcggcgcgga cggcgattat caaaggcggt
     2281 gccggggggg tcaaaccggc ccgtcgctcc gggaccacga cctttcgttc ccggacaaga
     2341 ggaatccctt gcgggaccag cgtttcgccc tagattagga cccattgagg ggaggcggga
     2401 gatgaagacg ctcgtccgcg tttccatgat ggccgtgctc gccgcgccgg cgctcgcgcg
     2461 agccgcgggc acgctcacgg gtgtgaccgc gagccccgcg ccggccgtgg ccggctccga
     2521 ggtgacgatc acggtcacgt cgaccggccc gtgtgcggtg tacgtcgatt tcggggacaa
     2581 gcagaaggcg gcgcacctct cgctcccgtc ccccgtgaaa cacgtctacg ccgcgccggg
     2641 aaagtacgtc atccggacct tcgcctacac gtccgggaac gaggcgccgg gcctgtcgag
     2701 gtgcggaggc ttcgccgaca tggagctcgt cgtgaaggcg gccgcgcccg tgatcgcacc
     2761 gaaccggaac ttcgttccgc ccgcgggggc gtcgtcgggc gtttcgaagg tgacgaatcc
     2821 cgcgccgtcc ggcggcggcg ccacggccga gccttcgggg gcggcacgta ccctcggcgg
     2881 gaaggtggcg ccaaccccga cagcgacgcc cacgaagatg gtcaggtgac gccagtggcc
     2941 ggagagtcag gaggcgtatg agaggccgcc cgagaatcgc cgcgcgggtc tgcgccctgg
     3001 gggttgcgct gctgcttccc cgtcctcttt acgggctccc cgtgagctgg gatgtccagg
     3061 caaagggcgt gttcacttac cgcgtcggct ccgagggggg tgtcggcaag cgcggtcagg
     3121 agaccacgac gttcctcgag ggggaggacg tgcagatcga gtgcctgtac ctcgtcaagc
     3181 agggcggcga caagaaggac ttcaccacct ggcaactggg gctgtccgtc gacggctccg
     3241 gcatgcattc gtggcccatg aacgccaacg tcgggacggt cgggggtgtc gggtccaagt
     3301 cctacgtctg gaaggcggac aaatccggat cacgcaagat caggtgcgcg gcggagacgt
     3361 acgatgcgga cccgtcgaac aacgcggcgg agataacggt cacggtgaag gcgcggcccc
     3421 cgcttacgga aatggaaatc ccgacacccg taccgcagag cccgccgccg ggaaagaaaa
     3481 tagcgctcgt tccgaacctg tacctcaccc tgagtgccca agttccacag aggcccgtcg
     3541 gtggctggcc gaaccttttc gactacgtca tccatcggcc gtaccagagc gtgaaatggc
     3601 acgtggacat cgtcgggccg tctcgtggcg gggcgatcag cctttcgacg cgtccggcgg
     3661 gtgacttcca gggcttcgtg agcaatctct cctacaagga cttcaacgcc tttgtcaccc
     3721 gtgactggct ggacgcccgt ggcctcggac ccgggcatta cgccgcgctc ttctacctga
     3781 gcgagaccgc aaacggcaag acgacgaacg gaaaaaaggg cgtcgtggag ttcgaactcg
     3841 ttgcgcctct catggccggc gcccagccgg cgggtggcgg cgcggcggca gggccctccg
     3901 acgcgatgcc tacgtcctcc ggaaaggcac cgaccccgac gcctgcgcgg cggaagtgac
     3961 ccggcgcgac ccgcccgacg gcggacgccg tgccgtgtgg cgcgtacaac agcgacggca
     4021 ccgcagagcg ccgtactggc cggcgccgca gaaggtggcg gcccgccgaa cgaatgcacc
     4081 gggtaggcgc cggaagtgaa ggtgacgctg aattccggcg tgatggccgc gccggcgatc
     4141 cgccgctgag gtgcggctga actctgtcaa cgctccttag aaatgtcccc tgaaatagct
     4201 cgttagaaat gtcccctc
//
LOCUS       DAOMFY010000321         4190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_7869_length_4190_cov_7.484160, whole genome shotgun sequence.
ACCESSION   DAOMFY010000321 DAOMFY010000000
VERSION     DAOMFY010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7869_length_4190_cov_7.484160"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLB01_17750"
     CDS             <1..876
                     /locus_tag="PLB01_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79197.1"
                     /translation="PRGVGQNPPAGAIVDVWLKDAPPKKPGPKDALTLEILDGETVLR
                     TFTSERKEAEDAPAEDEAEKPLELKQGLNRLSWDLRMLKPSLVPKAIIWGPRAGPLVA
                     PGTYTARVKQGGTVLAETIEAVANPMVKASAADLKKQSVFLASVRDRLSETHEAALTC
                     RDVKKQVKDVSERAARLGKKEPLAEKGKALSEKLTAIEEQLVNPKVKSEQDVLNFPPQ
                     LDHQFVGVASVAGSGEGAPLASSVAYFGQLEVRLADLKRQLQSVLDTDLADFNRTVAE
                     QGVPAVAPAPREGRR"
     gene            complement(873..1802)
                     /locus_tag="PLB01_17755"
     CDS             complement(873..1802)
                     /locus_tag="PLB01_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HQQ79198.1"
                     /translation="MPELPDVVVYAEHLERRLKGRTLRKVRLASPFVLRSVTPPLTDI
                     EGRAIRGVSRLGKRLVFSLEGEEGGAELFLVLHLMVSGRLRWKTAGTPIPIPKKIGLL
                     SFLFDHGTLLLTEASPKKRAWVRVVAGREALLALDPGGLEPLEASRDAFVARLRTENH
                     TLKRALTDPTLFSGIGNAYSDEILHAARLSPVALTSRLAGEEADRLFDATRTTLLDWT
                     ERLRRETGETFPEKVTAFREGMAVHGKFGRPCPRCGTAVQRIVYAANETNYCPACQTG
                     GKLLADRSLSRLLRGDWPKSLEEMEERIERSRS"
     gene            1819..3435
                     /locus_tag="PLB01_17760"
     CDS             1819..3435
                     /locus_tag="PLB01_17760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79199.1"
                     /translation="MPRCRKEETPAGSSPSSRAVRETLPPDLLAAGVRRLGTVALLMA
                     GSVLAFAVVERLVVARAGVAAFPGIVPFGQVSVVVVSLGMYAVTRRAGLDPAELLDRA
                     LVYEVLVGFLLAAVYHSVPDNPGVLPKGFSSVAVLIMAFPLLVPSTRGKTVLATVATA
                     LMDPIGLLVGIALGYRPAPSGSFAVAMFLPTAIAAIIAIIGSRIVYQLSAQAGKAEEL
                     GSYRLVAPLGRGGMGEVWRAEHRMLARDAAIKLVRGRDGGIARELMARFEREAKATAG
                     LRSPHTVQLYDFGRSDDGAFYYVMELLDGYTLDRLVERHGALAPERVVSVLRQACRSL
                     AEAHGAGLVHRDIKPGNIFLCRYGGEADFVKVVDFGLVKDVSASGGVNLTQTGLIAGT
                     PAYMPPEMALGRDVDGRADLYALGCVGYFLLTAQTVFDRKTALETLHDHTSTPPVPPS
                     RRVATPIPADLEAVLLDCLAKDPGDRPESALVLEERLAATSSAGLWSRADAERWWDRH
                     SPATGTYRPLPAEPEGTRLDTPEDRAAAAE"
     gene            3440..>4190
                     /locus_tag="PLB01_17765"
     CDS             3440..>4190
                     /locus_tag="PLB01_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQQ79200.1"
                     /translation="MCGRYWLVTPADVLASRFRLRGERVPLVPRWNAAPGQDLPVVRF
                     DAKGEGARILEPMRWGLVPSWAASPEDAGRPINARAETAATKPTFREPFRRSRALVPA
                     DGFFEWKKGDRGREPFALRLKSRRPFAFAGIFDTWTPASGPPLKSFAILTTRPNALVA
                     SVHDRMPVILTPEGEDLWLDPDARDPAALAPLLAPLGAEEMEAFAVSRRVNAPANDDP
                     SVLDAPGPEAPPPPKKSRASAAQRDLWENGGD"
BASE COUNT          599 a         1500 c         1507 g          584 t
ORIGIN      
        1 ccgcgcggcg tcgggcagaa cccgccggcc ggcgcgatcg tcgacgtctg gctgaaggac
       61 gcgccgccga agaagcccgg cccgaaggac gcgctcaccc tcgagatcct cgacggcgag
      121 accgtgctgc ggacgttcac ctcggaaagg aaggaggcgg aggacgcgcc cgccgaggac
      181 gaggcggaga agcccctcga gctcaagcag ggcctcaacc ggctgtcctg ggatctccgc
      241 atgctcaagc cgtcgctcgt gccgaaggcg atcatctggg gcccgcgtgc cggcccgctc
      301 gtcgcaccgg gcacgtacac ggcgcgcgtg aagcagggcg ggaccgtctt ggccgaaacg
      361 atcgaggccg tcgcgaaccc gatggtcaag gcctcggcgg cggacctcaa gaagcagtcc
      421 gtgttcctcg cgagcgtgcg ggacaggctc tcggagacgc acgaggcggc gctcacgtgc
      481 cgcgacgtga agaagcaggt gaaggacgtc tccgagcgcg cggcgcgcct cggcaagaag
      541 gagccgctcg ccgagaaggg caaggcgctt tcggaaaagc tcacggccat cgaggagcag
      601 ctcgtgaacc cgaaggtcaa gagcgagcag gacgtcctga acttcccgcc gcagctcgac
      661 caccagttcg tgggcgtcgc gagcgtcgcg ggaagcggcg aaggcgctcc cctcgcgtct
      721 tccgtcgcct acttcggcca gctcgaggtc cggctcgccg acctgaagag gcagctccag
      781 tccgtcctcg acacggacct cgcggatttc aacaggaccg tcgccgagca gggcgtcccg
      841 gccgtggcgc cggcgccgcg cgaggggagg cgctaggagc gggaccgctc gatccgctcc
      901 tccatctcct cgaggctctt cggccagtcg ccgcgcagga ggcgcgagag ggagcggtcc
      961 gcgaggagct tcccgccggt ctggcaggcg gggcagtagt tcgtctcgtt cgcagcgtag
     1021 acgatgcgct ggacggccgt cccgcagcgc ggacagggcc ggccgaactt tccgtggacg
     1081 gccatgccct cgcggaaggc cgtgaccttc tcggggaacg tctctcccgt ctcccgcctc
     1141 aggcgctccg tccagtcgag gagcgtcgtc cgcgtcgcgt cgaagaggcg gtcggcctcc
     1201 tcgcccgcga ggcgcgacgt gagggcgacg ggggagagcc gcgccgcgtg gaggatctcg
     1261 tccgagtagg cgttgccgat cccggagaag agcgtcgggt ccgtgagcgc gcgcttcaac
     1321 gtgtggttct ccgtgcgcag gcgcgcgacg aacgcatcgc gcgacgcctc cagcggctcg
     1381 aggccgccgg ggtcgagggc gagaagcgct tcgcggcccg cgacgacgcg cacccaggct
     1441 ctcttcttgg gactcgcttc cgtgaggagg agagttccgt gatcgaagag aaaagagaga
     1501 agaccgattt tctttggaat gggaatgggg gtgccggccg tcttccagcg cagccgaccc
     1561 gacaccatga gatggaggac caggaagagc tccgcgccgc cttcttcccc ttcaagagaa
     1621 aacacgagac gttttccgag ccgggacacc ccacgaatcg cacggccctc gatgtccgtc
     1681 agtgggggag tcaccgaccg caggacgaac gggctcgcga ggcggacctt gcgaagcgtc
     1741 cggcccttca ggcggcgctc gaggtgttcg gcgtagacga cgacgtccgg aagctccggc
     1801 atcggttcag aataggccat gccccggtgc cggaaagaag agacgcccgc cggttcgagc
     1861 ccgtcctcgc gcgcggtgcg cgagacgctg ccgcccgacc tccttgcggc gggtgtccgg
     1921 cgcctcggca ccgtcgcgct cctcatggcg gggagcgtcc tcgcgttcgc ggtcgtggag
     1981 cggctggtcg tggcgcgcgc cggagtggcc gcgttccccg gcatcgtgcc cttcggtcag
     2041 gtctccgtcg tcgtcgtgtc gctcggcatg tacgcggtga cgcgccgcgc gggcctcgac
     2101 cccgccgagc tgctcgaccg cgcgctcgtc tacgaggtgc tcgtcgggtt cctgctcgca
     2161 gcggtctacc actccgtgcc cgacaaccct ggagtcctgc cgaaggggtt ctcgtccgtg
     2221 gccgtcctga tcatggcgtt cccgctcctc gtaccgtcca cgcgcgggaa gaccgtcctc
     2281 gcgacggtcg cgaccgcgct catggacccg atcgggctgc tcgtcgggat cgcgctcggc
     2341 tatcgaccgg ccccttcggg gtccttcgcg gtcgcgatgt tcctgccgac ggccatcgcc
     2401 gccatcatcg cgatcatcgg gtcgcggatc gtgtaccaac tctcggcgca ggccgggaag
     2461 gccgaggaac tcgggagcta caggctcgtc gcgccgctcg ggcgcggcgg catgggcgaa
     2521 gtctggcgcg ccgagcaccg catgctcgcg cgcgacgccg cgatcaagct cgtgcgcggg
     2581 cgcgacggcg gaatcgcacg cgagctcatg gcgcgtttcg agcgcgaggc gaaggcgacg
     2641 gccggcctgc gctcgcccca tacggtccag ctctacgact tcggccggtc ggacgacggc
     2701 gctttctact acgtcatgga gcttctcgac ggctacacgc tcgaccggct cgtcgagcgc
     2761 cacggggcgc tcgcgcccga gcgggtcgtg tccgtcctcc ggcaggcgtg ccgctcgctc
     2821 gccgaggcgc acggcgcggg gctcgtgcac cgcgacatca agcccggcaa catcttcctc
     2881 tgccgctacg gcggcgaggc ggacttcgtg aaggtcgtcg acttcggcct cgtgaaggac
     2941 gtctcggcga gcggaggggt gaatctcacc cagacggggc tcatcgccgg gacgccggcc
     3001 tacatgccgc ccgagatggc gctcggcagg gacgtggacg gacgcgcgga cctctacgcc
     3061 ctgggctgcg tcggctactt cctcctcacg gcccagacgg tgttcgaccg caagacggcg
     3121 ctcgagacgc tgcacgacca cacctccacg ccgcccgtgc cgccctcgcg gcgcgtcgcg
     3181 acgccgatcc cggccgacct cgaagccgtg ctgctcgact gccttgccaa ggatcccggg
     3241 gaccgtcccg agagcgccct cgtcctcgaa gagcgtctcg cggcgacgtc gtcggcgggc
     3301 ctctggtctc gggcggacgc cgagcgctgg tgggaccgcc actcgccggc gacgggcacc
     3361 taccggccac tcccggccga gccggagggc acgcgcctcg acacgccgga agaccgcgcc
     3421 gccgcggcgg agtgacgccg tgtgcggccg atactggctc gtcacacccg ccgacgtcct
     3481 cgcgtcgcgc ttccgcctgc gcggtgagcg cgtcccgctc gtcccgcgct ggaacgcggc
     3541 gccgggacag gaccttcccg tcgttcgctt cgacgcgaag ggggagggcg cgcgcatcct
     3601 cgagcccatg cggtggggtc tcgtcccgtc gtgggcggcg agcccggagg acgcgggccg
     3661 cccgatcaac gcgcgcgccg agaccgcggc gacgaagccc acgttcagag agccgttccg
     3721 ccggagccgc gcgctcgtcc cggcggacgg attcttcgag tggaagaagg gcgaccgggg
     3781 ccgcgagccc ttcgccctgc gcctgaagtc gcggcgcccg ttcgcgttcg cggggatctt
     3841 cgacacgtgg acgcccgcga gcggcccgcc gctcaagtcg ttcgcgatcc tcacgacccg
     3901 gccgaacgcg ctcgtggcga gtgtgcacga ccggatgccc gtcatcctca cgcccgaggg
     3961 cgaggacctg tggctggacc cggatgcgcg ggaccccgcg gcgctcgcgc cgctgctcgc
     4021 gccgctcggg gccgaggaga tggaggcgtt cgcggtgtcg cgccgcgtga acgcgcccgc
     4081 gaacgacgac ccctcggtcc tcgacgcgcc cgggcccgag gccccgccgc ccccgaagaa
     4141 gagccgggcg tcggccgcgc agcgcgacct ctgggagaat ggcggggatg
//
LOCUS       DAOMFY010000322         4132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8041_length_4132_cov_6.428011, whole genome shotgun sequence.
ACCESSION   DAOMFY010000322 DAOMFY010000000
VERSION     DAOMFY010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_4132_cov_6.428011"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1529
                     /locus_tag="PLB01_17770"
     CDS             60..1529
                     /locus_tag="PLB01_17770"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79201.1"
                     /translation="MGNSILGEILEAKRRRLAAGEYRMAAAPGARPDGDRFAASLSSP
                     SKKSLSSSSPPSSLSFLCEIKHRSPSAGVILPDADARIEDVARAYRRGSAAALSIVVE
                     QDFFGGDPAWLPRAKAASGLPVLMKDFVVDETQLDFAAALGADAVLLIAAALGDADLA
                     RLHAGAKARGLAVLVEAHDEAEIRRAAALGAEIVGVNARDLSTFAVDLAGMARVGGLL
                     PRAATRVAESGIRTRGDVEALEAAGYGAFLVGETLLRSADPARTLRELRGENTTEVKI
                     CGVTREEDVDACLENGVDWIGLIFAARSPRKVTPDEGRFLRMKAKGEGGARRGGDGAP
                     GAVKGVVAVFDDDVADADVARIVSVVRPDAIQMPFPLSSPSKKFFSSSLPLWNTVRVG
                     RDDLAGVGNRAGAALHFDTSVAGASGGTGKTFDWGVLGGVERTRPLVLAGGLNPGNVA
                     EAVRRVAPDVVDVASGVEASPGVKDPSKIAAFVREVRRA"
     gene            1522..2730
                     /gene="trpB"
                     /locus_tag="PLB01_17775"
     CDS             1522..2730
                     /gene="trpB"
                     /locus_tag="PLB01_17775"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQQ79202.1"
                     /translation="MPDIPFRPPEADAHGRFGAFGGRFVPETLMAPLDELAKAYKDAR
                     RDRAFRAELENLLKTYVGRPTPLTEARCLSDALGGARLWLKREDLLHTGAHKINNAIG
                     QALLTRRMGKTRVIAETGAGQHGVATATACALLGLECVVYMGAVDVERQALNVYRMKL
                     LGATVVPVTGGSRTLKDAINEAMRDWVATTATTHYILGSVLGPHPFPTMVRDFHSVIG
                     REARAQCRKAFGRLPDLLVACVGGGSNAIGLFREFLADAGVEMVGVEAGGRSLEPGEH
                     AARFGPGSVGVLHGTRTLILQDAAGQVMPTHSVSAGLDYPAVGPEHARLAELGRTRYV
                     SVSDADALAAFHRLAETEGIIPALETAHAVAWAIAEAPRRPGKNIVVNLSGRGDKDVV
                     SVAKLEGVSL"
     gene            2727..3536
                     /gene="trpA"
                     /locus_tag="PLB01_17780"
     CDS             2727..3536
                     /gene="trpA"
                     /locus_tag="PLB01_17780"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQQ79203.1"
                     /translation="MTRLGRAFARAKSAGRAALVIFVEAGDPDLATTSRLLPALAEAG
                     ADVIELGIPFSDPLADGPTIQRASERALASGVTLAKVLDLLAASRAEGFATPVVVFSY
                     ANPILAMGEADFALRAREAGADGVLVTDLPPEEGRAFASILKSARLDPVYLLAPTSPD
                     DRLRRAAALSRGFVYLVSRSGITGARAALPEDLSALVGRVRRAVPRLPVAVGFGISTP
                     EQVRAVAALADGVVVGSAVVAAMETAVREGRDPVISAAELVRTLAGAAAKP"
     gene            3546..>4132
                     /locus_tag="PLB01_17785"
     CDS             3546..>4132
                     /locus_tag="PLB01_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79204.1"
                     /translation="MEEPLSGRISRADRKGLVELVAGHLARLTERDALQALRHPHASE
                     AMIVEIVASRVLMASRGVRRLVAGHPQTPRHDALHALDDLTWRDLMDIGRETRTPPPV
                     RQAANRKLLEALRGLAVGEKIALARFAPPALFPALFEEEDGRVLEALLSNPRLTPEDL
                     LRWLSTGHPTPGGLAQLAAEGRWVSRPPVRSALLL"
BASE COUNT          538 a         1500 c         1507 g          587 t
ORIGIN      
        1 aagcaagtga gaggggagag agagaagggg ggagagaaga gaagagggga gagagacggg
       61 tggggaactc gatactggga gagatcctcg aggcgaagcg tcggcgcctc gcggcgggtg
      121 agtacaggat ggcggccgcg cccggtgccc ggccggacgg agatcgcttc gcagcctccc
      181 tttcttcacc ttctaagaaa tctctctctt cttcttctcc tccttcttct ctctccttcc
      241 tctgcgagat caagcaccgc tccccgagcg cgggcgtgat cctcccggat gcggacgccc
      301 gcatcgagga cgtcgcgcgc gcctaccggc gcgggagcgc ggctgcgctt tcgatcgtcg
      361 tcgagcagga cttcttcggc ggggacccgg cctggctgcc gcgggcgaag gccgcgtcgg
      421 gcctgcccgt cctcatgaag gacttcgtcg tcgacgagac gcagctcgac ttcgcggccg
      481 cgctcggcgc ggacgcggtg ctcctgatcg cggcggccct cggcgacgcc gatctcgcgc
      541 gcctccacgc gggcgcgaag gcgcgcggcc tcgcggtcct cgtcgaggcc cacgacgagg
      601 ccgaaatccg ccgtgccgcc gcgctcgggg ccgagatcgt cggcgtgaac gcgcgggatc
      661 tctcgacgtt cgcggtggat ctcgccggaa tggcgcgcgt cggcggcctc ctgccgcgcg
      721 cggcgactcg agtcgccgag agcgggatcc ggacgcgggg ggacgtcgag gcgctcgagg
      781 cggcgggcta cggcgcgttc ctcgtcggcg agacgctcct gcgctccgcc gatcccgccc
      841 gcaccctgcg cgaactgcgc ggggagaaca cgaccgaggt gaagatctgc ggcgtcacgc
      901 gcgaggagga cgtcgacgcg tgcctcgaaa acggcgtgga ctggatcggc ctcatcttcg
      961 cggcgcgctc tccgcggaag gtgacgccgg acgaggggag atttctgcga atgaaggcga
     1021 agggggaggg cggcgcgcgg cgagggggcg acggcgcgcc tggcgccgtc aaaggagtgg
     1081 tcgcggtctt cgacgacgac gtggccgatg ccgacgtcgc gcgcatcgtc tccgtcgttc
     1141 gccccgacgc gattcaaatg ccctttccgc tctcttcacc ttctaaaaaa ttcttctctt
     1201 cctccctccc cctctggaac acggtccgcg tcggccgcga cgacctcgcg ggcgtgggaa
     1261 atcgcgcggg tgccgcactg cacttcgaca cgtccgtggc cggagcgagc ggcggcacgg
     1321 ggaaaacgtt cgactggggc gtgctcggcg gcgtcgagag gacgcggccg ctcgtgctgg
     1381 cgggaggcct gaatcccgga aacgtcgccg aggccgtgcg ccgcgtcgcg ccggacgtcg
     1441 tcgacgtcgc gtccggcgtc gaggcgtcgc cgggcgtgaa ggacccgtcg aagatcgccg
     1501 cgttcgtccg ggaggtgcgc cgtgcctgac attccgttcc gcccgccgga ggccgacgcg
     1561 cacgggcgct tcggcgcgtt cggcgggcgc ttcgtccccg agacgctcat ggcgccgctc
     1621 gacgagctcg cgaaggccta caaggacgcg cggcgcgacc gcgcgttccg cgccgagctc
     1681 gagaacctcc tgaagaccta cgtggggcgg ccgacgcccc tcacggaggc gcggtgcctc
     1741 tcggacgccc tcggcggcgc gcggctctgg ctcaaacgcg aggacctcct gcacacgggt
     1801 gcgcacaaga tcaacaacgc gatcggccag gcgctcctca cgaggcgcat gggcaagacc
     1861 cgcgtgatcg ccgaaacggg cgcggggcag cacggcgtcg cgacggcgac cgcctgcgcg
     1921 ctcctcggcc tcgagtgcgt cgtctatatg ggcgcggtcg acgtggagcg gcaggccctc
     1981 aacgtctacc gcatgaagct cctcggcgcg accgtcgtgc cggtgacggg cggctcgcgc
     2041 accctcaagg acgcgatcaa cgaggcgatg cgggactggg tcgcgacgac cgcgacgacg
     2101 cactacatcc tcggctcggt cctcgggccg cacccgttcc cgaccatggt gcgcgacttc
     2161 cattccgtca tcggaaggga ggcgcgggcc cagtgccgga aagccttcgg ccgccttccc
     2221 gacctcctcg tcgcgtgcgt cgggggcggg tcgaacgcga tcggcctctt ccgcgagttc
     2281 ctcgcggacg ccggtgtcga gatggtcggg gtcgaggcgg gggggaggag cctcgagccg
     2341 ggcgagcacg cggcgcgctt cgggccgggc tccgtcggcg tcctgcacgg cacacggacg
     2401 ctcatcctgc aggacgcggc cgggcaggtg atgccgacgc actccgtgtc ggccggcctc
     2461 gactatcccg cggtcggacc cgagcacgcg cgtctcgccg agctcgggcg cacgcgctac
     2521 gtctccgtgt cggacgcgga cgcgctcgcg gcctttcacc gcctcgcgga gaccgagggg
     2581 atcatcccgg ccctcgagac ggcgcacgcc gtcgcgtggg ccatcgccga ggcgccgcgc
     2641 cggcccggga agaacatcgt cgtgaacctg tccgggcgcg gggacaagga cgtcgtgtcg
     2701 gtcgcgaagc tcgagggagt gtccctgtga cgcgcctcgg ccgcgcgttt gcgcgtgcga
     2761 agagcgcggg gcgggccgcg ctcgtgatct tcgtcgaggc cggcgacccc gacctcgcga
     2821 cgacgagccg gctgctgccg gcgctcgcgg aggccggcgc cgacgtgatc gagctcggca
     2881 tcccgttcag cgaccctctc gcggacggcc cgacgatcca gcgtgcgtcc gagcgcgcgc
     2941 tcgcctcggg cgtgacgctc gcgaaggtcc tcgacctcct cgccgcctcg agggccgagg
     3001 gattcgcgac ccccgtcgtc gtcttctcgt atgccaaccc gatcctggcg atgggggagg
     3061 ccgacttcgc gctccgcgcg cgcgaggccg gggcggacgg cgtcctcgtg acggacctcc
     3121 cgcccgagga ggggcgcgcc ttcgcgtcga ttctcaagag cgcccgcctc gaccccgtct
     3181 acctcctcgc gcccacgtcg cccgacgacc gcttgcgccg cgcggcggct ctctcccgcg
     3241 gcttcgtgta cctcgtgtcg cgaagcggaa tcacgggcgc gcgcgccgcc cttcctgagg
     3301 acctctcagc cctggtcggg cgcgtccgcc gcgcggtgcc gcgccttccc gtcgcggtcg
     3361 ggttcggcat ctcgacgccg gaacaggtcc gggccgtcgc cgctctcgcg gacggcgtcg
     3421 tcgtcgggag cgccgtcgtc gccgcgatgg agacggcggt ccgcgagggc cgcgaccccg
     3481 tgatctccgc cgcggagctc gtccggactc tcgccggagc ggccgcgaag ccgtaaagtt
     3541 cggccatgga ggagcccctt tccgggcgga tttcccgggc ggaccgaaag ggcctcgtgg
     3601 agctcgtggc cgggcacctc gcgcgcctga ccgagcggga cgcgctgcag gcgctccggc
     3661 acccgcacgc ctccgaggcg atgatcgtcg agatcgtggc gtcgcgcgtc ctcatggcgt
     3721 cgcgcggcgt gagaaggctg gtcgcggggc acccgcagac accgcggcac gacgccctcc
     3781 acgcgctcga cgacctgacg tggcgcgacc tgatggacat cggccgggag acgcggacgc
     3841 cgccgcccgt gaggcaggcg gccaaccgca agctcctcga ggccctgcgc ggattggccg
     3901 tcggcgagaa gatcgcgctc gcccggttcg cccccccggc cctgttcccg gcgctcttcg
     3961 aggaggaaga cgggcgcgtc ctcgaggcgc tcctctcgaa ccctcgactc acgcccgagg
     4021 acctcttgcg ctggctctcg accgggcacc cgactcccgg aggcctcgct cagctcgccg
     4081 cggagggccg ttgggtctcc cgcccgcccg tgcgcagcgc cctgctcctg ca
//
LOCUS       DAOMFY010000323         4123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8068_length_4123_cov_7.660767, whole genome shotgun sequence.
ACCESSION   DAOMFY010000323 DAOMFY010000000
VERSION     DAOMFY010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8068_length_4123_cov_7.660767"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLB01_17790"
     CDS             complement(<1..867)
                     /locus_tag="PLB01_17790"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HQQ79205.1"
                     /translation="MRTWSYESEPYRTSLATEVVEAGVEAGKPFAVLEDTILYPEGGG
                     QPADRGTLNGVAVLDVQKKDGAIRHVLASAVPPGPADLRLDWERRFDHMQQHTGQHLL
                     SAVAQDRFRWETTAFHLGPEVSDVELAVTSLSADDLARLEEAVAAEIRAARPVVPRRV
                     SLEEFRTLPVRTRGLPEGFSGDVRLVEISGVDLNTCGGTHLANTAEIEVVALLATESL
                     RGGTRVFFAAGGRARRRLKHHEDRNAALRVAIGAADDALVDAAKAKREQLSEAQKALR
                     RAEDSLAEAAAEA"
     gene            complement(864..1748)
                     /locus_tag="PLB01_17795"
     CDS             complement(864..1748)
                     /locus_tag="PLB01_17795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HQQ79206.1"
                     /translation="MRRVSPSALAVLLVLSVPAPAQKPECSVKAPRGASEAELRRLTK
                     VSVKDAEAKAIASVAPDKVNSIVSGDVDVLDGCLAWTFLLRFSNKGGVTEVSVDAGDG
                     KILSSVYEPPKGVSAAPAASPDAATPGAAPAPDARPVFDPAAEEPALRKAVDDEEAAW
                     NRADAKALAAAFRDDGTYADAFGGVSRGRAEVDKRLAEVLATWRGTRLALKVRKVRVL
                     KPDVALVEADSELTGWKKLPPGFRAEDGQTLRSRLLQVFQKEAGAWRVAASYEVDVKT
                     KLELPPVPEERTIRGRAR"
     gene            complement(1769..2950)
                     /locus_tag="PLB01_17800"
     CDS             complement(1769..2950)
                     /locus_tag="PLB01_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ79207.1"
                     /translation="MEQDDQERIRGEAQRRQASRMEAVGQLAGGIAHDFNNLLTAILG
                     YAQLLSERPLGDAALAEVAEIRHAAESAASLTRQLLAFSRQQVQKISVFVLNDVVSET
                     ESMLARLLGEDVRICKELAPGLGRVKADPGQIEQVLVNLAVNARDAMPKGGTLRIETR
                     DVDLDEAYVDTHVAGTPGPYVMLAMSDDGCGMDPATLARIFEPFFTTKPVGKGTGLGL
                     ATVYGIVKQSGGFVWVYSDPGHGTTFRIYLPRVEEPLSARRDSGTPRRTPPKGTETIL
                     LVEDDDAVRRFVRDVLVRNGYTVHAAASGNEAVNAARPIAPAIHLVLSDVVMPEMSGA
                     VLRRRLEALGVKARVLFMSGHSADAVAVRGIEAGDGAFLQKPFTPGDLLFKVRRVLDG
                     G"
     gene            3148..3642
                     /locus_tag="PLB01_17805"
     CDS             3148..3642
                     /locus_tag="PLB01_17805"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ79208.1"
                     /translation="MDGAAGTRPDEWDWERLDGHLRPRLIAVGIRRFGCTPEEAEDLV
                     QDIFEAVLVKRPRVRNTEGYLVSTFFNRCANRVEQRARHLELEALLGGASPGTNGSER
                     LLTAVHVRGALRRLTPVCRRLIRSYCVQRISLAEAAADSGCSVPAVWKRINQCIKRMR
                     TCLA"
     gene            3630..>4123
                     /locus_tag="PLB01_17810"
     CDS             3630..>4123
                     /locus_tag="PLB01_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79209.1"
                     /translation="MSGLSPHPAEVELLALALGRDGDAAENARIHVDGCPTCRRRETA
                     LSEVAAAIELDHLLHEELDAAEPFSASPQSASGRKRIALLAADSDSALRDAEALLELA
                     RDARADLAGRVRELAAGNVGRLTLLYAVQRGAFAAAVPARALALAEAVAAAADGQEPP
                     AQAGA"
BASE COUNT          562 a         1509 c         1448 g          604 t
ORIGIN      
        1 cgcctccgcc gccgcctcgg ccagggagtc ctcggcgcgc cgcagcgcct tctgggcctc
       61 ggagagctgt tcccgcttcg ccttcgcggc gtcgacgagc gcgtcgtcgg ccgcgccgat
      121 cgcgacgcgg agggccgcgt tccggtcctc gtggtgtttc agccggcgcc gggcgcggcc
      181 gcccgcggcg aagaacacgc gcgtcccgcc gcgcagcgac tccgtcgcga gaagcgcgac
      241 gacctcgatc tcggcggtgt tcgcgaggtg cgtcccgccg cacgtgttga ggtccacgcc
      301 cgagatctcg acgaggcgca cgtcgcccga gaagccctcg ggcaggccgc gcgtcctcac
      361 gggcagcgtc cggaactcct cgagcgacac gcgccgcggc acgaccgggc gcgcggcccg
      421 gatctccgcg gcgacggcct cctcgagccg cgcgaggtcg tcggccgaga gggaggtcac
      481 ggcgagctcg acgtcggaca cctcgggccc gaggtggaat gcggtcgtct cccagcggaa
      541 ccggtcctgt gccactgccg agagcaggtg ctggcccgtg tgctgctgca tgtggtcgaa
      601 gcggcgctcc cagtcgagcc gcaggtccgc gggccccggc gggaccgcgg acgcgagcac
      661 gtggcggatc gcgccgtcct tcttctgcac gtcgaggacg gcgacgccgt tcagcgttcc
      721 gcggtcggcc ggctggccgc cgccctcggg gtagaggatc gtgtcctcga ggacggcgaa
      781 cggcttcccc gcttccacgc cggcctcgac gacctccgtc gcgagcgacg tgcggtacgg
      841 ctcgctctcg tacgaccagg tcctcatcgg gcccggcccc ggatcgtgcg ctcttccgga
      901 accggcggaa gctcgagctt cgtcttgacg tccacctcgt atgaagccgc gacgcgccag
      961 gctcccgctt ccttctggaa gacctgaagg aggcggctgc gcagggtctg accgtcctcg
     1021 gcgcggaagc cgggcgggag cttcttccag ccggtgagct cggaatcggc ctcgacgagc
     1081 gcgacgtccg gcttcaggac gcggaccttg cgcaccttga gcgcgaggcg ggtgccgcgc
     1141 caggtcgcca ggacctcggc gagccgcttg tcgacctccg cgcgcccgcg cgagacgccg
     1201 ccgaacgcgt ccgcatacgt cccgtcgtcg cgaaacgccg ccgcgagcgc cttcgcgtcc
     1261 gcgcggttcc acgccgcttc ctcgtcgtcg acggccttgc ggagcgcggg ctcttccgcc
     1321 gccgggtcga acacggggcg ggcgtcggga gccggagccg cgccgggggt tgccgcgtcc
     1381 ggcgacgcgg caggcgcggc cgacacgccc ttcggcggct cgtacaccga cgacaggatc
     1441 ttcccgtcac ccgcgtccac ggacacttcg gtcacgccgc ccttgttcga gaagcggagg
     1501 agaaacgtcc aggcgagaca tccgtcgagg acgtccacgt cgcccgacac gatcgaattc
     1561 accttgtccg gggcgaccga agcgatcgcc ttcgcctcgg cgtccttcac gctcaccttc
     1621 gtgagacgcc ggagctcggc ctcggacgcg ccgcggggcg ccttgaccga gcattccggc
     1681 ttctgggcgg gcgcggggac ggacaggacg aggaggacgg cgagggcgga gggcgagacg
     1741 cgacgcatcg cccgattatc gttcgccgtc acccgccgtc cagcaccctc cgcactttga
     1801 agaggaggtc ccccggagtg aacggcttct ggaggaacgc gccgtcgccg gcctcgatgc
     1861 cgcggacggc gaccgcgtcc gcggagtggc ctgacatgaa cagcacgcgc gccttcactc
     1921 cgagagcctc gagtcggcgc cgcaggaccg ccccactcat ttccggcatc acgacgtcac
     1981 tcaagacgag gtggatcgca ggggcgatcg ggcgtgcggc gttgacagcc tcgttgcccg
     2041 aagcggccgc gtggacggtg tatccgttgc gcacgaggac gtcgcgcacg aaacggcgga
     2101 cggcgtcgtc gtcctcgacg aggaggatcg tttcggtccc cttcgggggc gtgcgccggg
     2161 gagtgcccga gtcccggcgc gccgagaggg gctcctcgac gcgcggcagg tagatccgga
     2221 acgtcgtgcc gtggcccggg tcgctgtaca cccagacgaa gccgccgctc tgtttgacga
     2281 tcccgtagac cgtcgcaagc ccgagccccg tccccttccc cacgggcttc gtcgtgaaga
     2341 agggctcgaa gatccgcgcg agcgtcgccg ggtccatccc gcagccgtcg tcgctcatgg
     2401 cgagcatgac gtacggcccc ggcgtcccgg cgacgtgggt gtcgacgtac gcctcgtcga
     2461 ggtccacgtc gcgcgtctcg atacggagcg ttccgccctt cggcatcgcg tcgcgcgcgt
     2521 tcacggcgag gttcacgagc acctgctcga tctggccggg gtccgccttc acgcgtccga
     2581 ggccgggagc cagttccttg cagatgcgga cgtcctcgcc gaggagccgc gcgagcatcg
     2641 actccgtttc cgacacgacg tcgttcagga cgaagaccga gatcttctgg acctgctggc
     2701 ggctgaacgc gaggagctgc cgcgtcagcg aggcggcgct ctcggcggcg tgacggatct
     2761 cggcgacttc ggccagggcg gcgtcaccca gcggacgctc cgacaggagc tgggcgtaac
     2821 caaggatcgc ggtgaggagg ttgttgaagt cgtgtgcgat cccgccggcg agctggccga
     2881 cggcttccat ccgggacgcc tgcctgcgct gcgcctcgcc ccggatccgc tcctggtcgt
     2941 cctgctccac tcggtctccc cggaggatcc ggtcgggcgg ccggagggcc ctccatctct
     3001 atagacagtt ggcggggggc gttttcaacc accctcgatg tccttgaact aagggctcgt
     3061 cggacgtacg ctgaatcgaa ggtcgaggtg tatatgtcgc ccggcgtcag ccaagacggc
     3121 attttttcgg acccggatcc gcccttcgtg gacggggccg caggcacgcg accggacgag
     3181 tgggactggg aacggctcga cggccacctg cggccccgcc tcatcgcggt cggcatccgg
     3241 cgcttcggct gcacgcccga ggaggcggag gacctcgtcc aggacatctt cgaggccgtg
     3301 ctcgtcaagc gcccccgtgt gcgcaacacc gagggctatc tcgtgagcac gttcttcaac
     3361 cgctgcgcca atcgcgtgga acagcgcgcc cgccacctcg agctcgaggc cttgctcggc
     3421 ggcgcgtccc cggggacgaa cggctccgaa cgcctcctga ccgccgtgca cgtgcgcggc
     3481 gcgctccgtc gcctgactcc cgtctgccgc cgcctgatcc gctcctactg cgtccagagg
     3541 atctcgctcg cggaggcggc cgcggactcg gggtgcagcg tgcccgccgt gtggaagcga
     3601 atcaaccagt gcatcaagag gatgagaacg tgtctggcct gagccctcac ccggccgagg
     3661 tcgaactcct cgccctggcc cttgggcgcg acggcgacgc cgcggagaac gcccggatac
     3721 acgtcgacgg gtgtccgacg tgccgacgcc gcgaaaccgc gctgagcgag gtcgcggccg
     3781 ccatcgagct cgaccatctt ctccacgagg agctcgatgc cgccgagccc ttctccgcgt
     3841 cgccgcagtc cgcctccggc cggaaacgca tcgcgctcct cgccgcagac tcggattccg
     3901 cgctccgcga cgccgaagcg cttctggaac tcgcccgcga tgcccgggcc gacctcgcgg
     3961 gaagagtccg ggagctcgcg gccgggaacg tgggccgcct cacgctgctc tacgccgtac
     4021 agcgcggcgc gttcgcggcg gccgtcccgg cgcgcgcact cgccctcgca gaagcggtcg
     4081 ccgccgccgc ggacggccag gagcctccgg cgcaggccgg ggc
//
LOCUS       DAOMFY010000324         4103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8126_length_4103_cov_7.291255, whole genome shotgun sequence.
ACCESSION   DAOMFY010000324 DAOMFY010000000
VERSION     DAOMFY010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8126_length_4103_cov_7.291255"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1465)
                     /gene="asnS"
                     /locus_tag="PLB01_17815"
     CDS             complement(176..1465)
                     /gene="asnS"
                     /locus_tag="PLB01_17815"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQQ79210.1"
                     /translation="MTISELSRHVGGTVTLKGWLYHWRKGGKIWFLVLRDGSGYLQCV
                     VSKADVPEAVWEAATSATQESTLEITGVVKADPRSPGGVELGVTDVKVLDLCHDFPIT
                     PKEHGVDFLMSQRHLWLRSSKQVAVLKVRSEVESAIHDFFYTRGYTRVDSPILTPAAC
                     EGTSNLFETQYTEEEKAYLSQSGQLYLEPAAAALGKVYCFGPTFRAEKSKTRRHLREF
                     WMVEPEVAFLEVDGLMDLAEEFVKELTARVLDRRAEDLKRLERDVTKLEPVASKRFPR
                     ISYREAVDILKGKGFPVQFGDDLGGDEETALTEAFDTPVMVTRYPTSIKAFYMQPAPD
                     DPDVVLGLDMLAPEGYGEIIGGSQRIHDHDLLLSRIQQHNLPLEAFQWYLDVRKYGTF
                     PHSGFGMGLERFVAWMCGVPHLRECIPYPRMLYRIYP"
     gene            complement(1462..1716)
                     /locus_tag="PLB01_17820"
     CDS             complement(1462..1716)
                     /locus_tag="PLB01_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79211.1"
                     /translation="MANPAWKVAADVAEGYVSLNPVVLKKYEKHEVDALMKELEKITR
                     DTRSQVVPIDDADASAKRSRRLLRLSQANVVLQSWRARMK"
     gene            1906..3054
                     /locus_tag="PLB01_17825"
     CDS             1906..3054
                     /locus_tag="PLB01_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQQ79212.1"
                     /translation="MLSEDEKAVRDLVRDFVDAEVLPIIEECAYEGRFPKELIPQMAA
                     MNLFGSTIPEYGLPGLNNVAYGLITQELERGDSGLRSFVSVQSALVMYPIYTYGSKEQ
                     KDRWIPALARGEAIGCFGLTEPDFGSNPGGMRTVAKKDGKDWILNGAKAWITNGSIAD
                     VAVVWAKVGGADGPIRGFLVPRGTKGFTAPEHRMKMSLRASVTSQLAFEDVRLPEDAV
                     LPGVEGIKGPLSCLTQARYGIAWGGLGSAMATYTCALDYAKSRKQFKDRPIASHQLVQ
                     QKLAFMITEITKGQLLALRLGRMKDAKTMKPEHVSMAKRNNVWVARECAKLAREILGA
                     NGIVGEYPVFRHLANIESVFTYEGTHDIHTLVLGQAVTGIPSYNPPME"
     gene            3095..>4103
                     /locus_tag="PLB01_17830"
     CDS             3095..>4103
                     /locus_tag="PLB01_17830"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HQQ79213.1"
                     /translation="MAIAGNLRTMQLSELLQWLSTGLKSGTLVVRGAPGEKRIYFEKG
                     RVTSSSSTLEREHLGRFLVGFGFITEEELIRALEVQQESRILLGKILVMIGAIKEAEL
                     ADLVRLKAAETIYDIFLWNEGSFAFIDGEVPQLPMVPISSDVTGIVMEGLRRYDEWQR
                     IKTRITSMRQIPSLIVPVEEALGERDKLILGAINGRRAIDQIALETHNPEFHVAKLVF
                     DLMLSGHLALAGEREDAAATPVFADAESEADAASAFDPILGASADDASPEPRPRVEEY
                     LLSPPGPRTPPPTDYGRFLKRGPDSDVTLRRKAGPGASNDTQPAVKTPPPVEPAIGSS
                     LA"
BASE COUNT          681 a         1383 c         1374 g          665 t
ORIGIN      
        1 tgacctcggt gtccacgagg ttcttcgggc agccgaggga gacgatgccc acggacggga
       61 gaagggaccg gggaccggaa gatttcatag aaggtttgac gggccgcggc ccgcctcgtc
      121 ggggaggccg accgcgtgcg cgaacgggca ttctaccttc gaagaaactc cggttctacg
      181 gatagatgcg atacagcatc ctcggatagg gaatgcactc cctcaggtgc ggcacgccgc
      241 acatccacgc gacgaagcgc tcgaggccca ttccaaagcc cgagtgcggg aacgtgccgt
      301 acttcctcac gtcgaggtac cactggaacg cctcgagcgg caggttgtgc tgctggatgc
      361 gcgagaggag aaggtcgtgg tcgtggatgc gctgggagcc gccgatgatc tcgccgtaac
      421 cctcgggcgc gagcatgtcg aggccgagga cgacgtccgg gtcgtccggg gcgggctgca
      481 tgtagaacgc cttgatggac gtcgggtagc gcgtgaccat gacgggcgtg tcgaaagcct
      541 cggtgagcgc ggtctcctcg tcgccgccga ggtcgtcccc gaactgcacc gggaagccct
      601 tccccttcag gatgtccacc gcctcgcggt aggagatccg cgggaaccgc ttcgacgcca
      661 cgggctcgag cttcgtcacg tcgcgctcga ggcgcttgag gtcttcggcg cggcggtcga
      721 ggacgcgcgc cgtgagctcc ttgacgaact cctcggcgag atccatcagc ccgtcgacct
      781 cgaggaacgc gacctcgggc tcgaccatcc agaactcgcg caggtggcgg cgggtcttgg
      841 atttctcggc ccggaacgtc ggcccgaagc agtagacctt cccgagggcg gcggccgcgg
      901 gctcgaggta gagctggccc gactgcgaga ggtaggcctt ctcttcttcc gtgtactggg
      961 tctcgaagag gttcgacgtg ccctcgcagg cggcgggcgt gaggatcggc gagtccacgc
     1021 gcgtgtagcc gcgcgtgtag aagaagtcgt ggatcgcgct ctcgacctcg ctccgaacct
     1081 tgaggacggc gacctgcttc gaggaccgga gccaaaggtg gcgctgcgac atcaggaagt
     1141 cgacgccgtg ctccttcggc gtgatcggga agtcgtggca gaggtcgagc accttcacgt
     1201 ccgtgacgcc cagctcgacg ccgcccggcg agcggggatc ggccttcacg acgcccgtga
     1261 tctcgagcgt ggactcctgg gtcgcgctcg tcgcggcctc ccagacggcc tcgggcacgt
     1321 ccgccttcga gacgacgcac tggagatagc cgctcccgtc gcggaggacc aggaaccaga
     1381 tcttgccgcc cttcctccag tggtagagcc agcccttgag cgtcacggtc ccgccgacgt
     1441 ggcgggacag ttcggaaatc gtcacttcat cctcgctctc cagctctgca ggaccacgtt
     1501 cgcctgcgag agccgcagca gcctccggct gcgcttcgcg ctcgcgtccg cgtcgtcgat
     1561 cggaacgacc tgcgagcggg tgtcccgcgt gatcttctcg agctccttca tgagggcgtc
     1621 gacctcgtgc ttctcgtact tcttgagaac cacggggttg agggagacgt agccctcggc
     1681 cacgtccgcc gccaccttcc acgcgggatt cgccatgcag cgattatgca gggaacgaag
     1741 agagagattt cttcgaatgg ttggggaggg tcgcggcgcc ccccaagggc tcagcggtgc
     1801 ccgccgctcc ggctgtattc accttctaag aaaatctccg atactctctc cccgcgtgga
     1861 ggcaacgtga gcttttccgg agtggatttc ctggggattg aagacatgct gtcggaggac
     1921 gagaaggcgg tccgcgacct cgtccgcgac ttcgtggacg ccgaggtcct cccgatcatc
     1981 gaagagtgcg cctacgaggg ccgcttcccg aaggagctga tcccgcagat ggcggcgatg
     2041 aacctcttcg ggtccacgat cccggagtac ggcctgccgg gcctcaacaa cgtcgcctac
     2101 ggcctcatca cgcaggagct cgagcgcggg gattcgggcc tgcgctcgtt cgtgtcggtg
     2161 cagtcggcgc tcgtcatgta cccgatctac acatacggct cgaaggagca gaaggaccgc
     2221 tggatccccg ccctcgccag gggcgaggcg atcggctgtt tcgggctcac ggagcccgac
     2281 ttcggctcca acccgggcgg gatgcgcacg gtcgcgaaga aggacggcaa ggactggatc
     2341 ctgaacggcg ccaaggcgtg gatcacgaac ggctccatcg cggacgtcgc ggtcgtctgg
     2401 gcgaaggtcg gcggcgccga cggcccgatc cgcggcttcc tcgtcccgag gggcacgaag
     2461 ggcttcacgg cccccgagca ccgcatgaag atgtcgctcc gggcgtccgt cacctcgcag
     2521 ctcgcgttcg aggacgtccg gctgccggag gacgcggtgc tcccgggcgt cgagggcatc
     2581 aagggccccc tgtcctgcct cacgcaggcc cggtacggga tcgcctgggg cggcctcggg
     2641 tcggccatgg cgacgtacac ctgcgcgctc gactacgcga agtcgcgcaa gcagttcaag
     2701 gaccgcccga tcgcctcgca ccagctcgtc cagcagaagc tcgcgttcat gatcacggag
     2761 atcacgaagg gccagctcct cgcgctgcgc ctcggccgga tgaaggacgc gaagacgatg
     2821 aagcccgagc acgtctccat ggccaagcgc aacaacgtgt gggtcgcccg ggagtgcgcg
     2881 aagctcgcgc gcgagatcct cggcgccaac gggatcgtcg gcgagtaccc cgtgttccgc
     2941 cacctcgcca acatcgagtc cgtctttacg tacgagggca cgcacgacat ccacacgctc
     3001 gtcctggggc aggcggtgac cggcatcccg tcgtacaacc ctccgatgga gtaaaccccc
     3061 tccgctcaga tcgttgcggt tacaatggac cgccatggcc atcgcaggaa atctgcggac
     3121 gatgcagttg tccgaattgc tgcagtggct gtccaccggc ctgaaatcgg gcacgctcgt
     3181 ggtccgcggc gcgccgggcg agaagcggat ctacttcgag aagggccgcg tcacgtcgtc
     3241 ctcctccacc ctcgagcgcg agcacctcgg ccggttcctc gtggggttcg ggttcatcac
     3301 cgaggaagag ctgatccgtg cgctcgaggt gcagcaggaa tcgcgcatcc tgctcgggaa
     3361 gatcctcgtg atgatcggcg ccatcaagga ggcggagctc gcggacctcg tgcgcctgaa
     3421 ggccgccgag acgatctacg acatcttcct ctggaacgag ggctcgttcg ccttcatcga
     3481 cggagaggtc ccgcagcttc cgatggtccc gatctcgtcg gacgtgacgg gcatcgtgat
     3541 ggagggcctc cgccgctacg acgagtggca gcgcatcaag acgaggatca cgtccatgcg
     3601 ccagatcccg tccctgatcg tgcccgtcga ggaggcgctc ggcgagcgcg acaagctcat
     3661 cctcggggcg atcaacggcc gccgcgcgat cgaccagatc gcgctcgaga cgcacaaccc
     3721 cgagttccac gtggcgaagc tcgtcttcga cctcatgctg agcggccacc tggctctcgc
     3781 gggcgagcgt gaggacgccg ccgccacgcc cgtcttcgcc gacgccgagt ccgaggccga
     3841 cgccgcgtcg gccttcgatc cgatcctcgg ggcctcagcg gacgacgcgt cgcccgagcc
     3901 gcgcccgcgc gtcgaggaat acctgctgtc tcctcccggg ccgcgcaccc ctccgcccac
     3961 ggactacggg cgcttcctca agcgtggacc cgattcggac gtcacgctgc ggcgcaaggc
     4021 ggggccgggc gcgagcaacg acacccagcc ggccgtcaag acgcctccgc cggtcgagcc
     4081 ggcaatcggc tcgtccctcg cgg
//
LOCUS       DAOMFY010000325         4003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8496_length_4003_cov_8.390831, whole genome shotgun sequence.
ACCESSION   DAOMFY010000325 DAOMFY010000000
VERSION     DAOMFY010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8496_length_4003_cov_8.390831"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLB01_17835"
     CDS             <1..305
                     /locus_tag="PLB01_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HQQ79214.1"
                     /translation="AAPAAVAAATAARLAAETLKASSAAAMSATIAAAAAGGASIHVC
                     ATPWPIPPHGPGVVIDGSATVSIENAPACRQGDTVVEAIGPPNKIVMGCTTVIIGG"
     gene            367..2535
                     /gene="tssI"
                     /locus_tag="PLB01_17840"
     CDS             367..2535
                     /gene="tssI"
                     /locus_tag="PLB01_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HQQ79215.1"
                     /translation="MPYTQSGRRLKVTTPLGPDVLLLNGFSGHEAISRPFLFRLECVA
                     ENDKAKQVVFDALLGQKVTAEIDLPGGAKRHVNGTCLRVAEGARDATFTRFTLDLVPE
                     PFLLSRRTQSRIFQQITVPDILKKVFAGFEMAWELQGTFEPRDYCVQYRESDFAFASR
                     LMEEEGIYYFFKHSDGSHKMVVANLPASHSDLPGKANLIFEAVGGGSREEDRVHAWEK
                     SQELKSSKVKLWDHCFELNHKQHLEAEFPIQAAVAAGKVSHKLKLGANAKLELYDFPG
                     GYAQRFDGIQPGGGDRPADIQKVFDDNKRTVELRMQEQAAASVLVHATSNCRQVVSGH
                     KFTLERHFDGDGPWVVYEVEHAASEAADLRSGGGGFTYQNRFTCFPVALPFRPPRVTP
                     IPTVKGTQTAVVVGPPGEEIFTDKYSRIKVQFHWDREGKNNSDSSCWVRVATPWAGQR
                     WGMIHIPRIGQEVVVDFLEGDMDQPIVVGSVYNAECMPPYDLPAHKTQSGIKSRSSLQ
                     GTPANFNEIRFEDKKGHEQLFIHAEKNQDIEVENDETHWVGHDRRKTIDHDETTHVKH
                     DRTETVDNNETITIGSNRTEMVGINETITIGANRTETVGANESISIGANRSTAVGGSE
                     SVAVGASRTTAIGQNAALQVGGNATTQVGKNDEIQVGKSFALIAGDQIQLQTGDASII
                     MKKDGTITIKGKDIKLVGSGKINVKADGDVVMKGSKISQN"
     gene            2551..3564
                     /locus_tag="PLB01_17845"
     CDS             2551..3564
                     /locus_tag="PLB01_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HQQ79216.1"
                     /translation="MLQLKNRTRLAATLFSSPDPDGVETLYAVVKGTFTLGPRVEEAE
                     KQTPIVVTDRFHGDPLKTSIAEPADIGLVKPGTDVLLFGHAYGPGGRPAARAEVSFRV
                     GPIEKRAVVFGDRVWGGTVLTRVSAPEPFERMPLVWERAFGGTEPPAGDPPRTDGDDR
                     NPVGTGFRSRMSGRKLEGIALPNIEDPAKLISSPGDRPSPMGFGAVAPHWKPRPKYAG
                     TYDEAWQKSRAPYLPQDFDPRFLQVAPEDQQVPGYLRGGEPVEIAGATPSGVLRFALP
                     ARRVDVLFHLDSGVERRIANLDTVLLHPDESRLVLVWRAALPCDKRLLKVREVEPIVS
                     RAA"
     gene            3572..3877
                     /locus_tag="PLB01_17850"
     CDS             3572..3877
                     /locus_tag="PLB01_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79217.1"
                     /translation="MAAPDVVVARVGMVTAVGLSAAETIASVRSATMRFEKIDWLDHR
                     FEPFTVAFVPDAGLPALAPDVAKTAGLSIRDARLLRLGAKALAECLGPARLGARLRA"
BASE COUNT          725 a         1394 c         1342 g          542 t
ORIGIN      
        1 cggcggcccc ggccgcggtc gccgcggcga cggccgcccg actggccgcc gagaccctga
       61 aggcgtcgtc cgcggccgcc atgagcgcga cgatcgccgc tgcggcagcg ggaggggcga
      121 gcatccacgt gtgcgcgacg ccctggccca tccccccgca cgggccgggc gtcgtcatcg
      181 acggctcggc gacggtctcg atcgagaacg cgcccgcctg ccggcaaggc gatacggtcg
      241 tcgaggcgat cggcccgccg aacaagatcg tgatgggctg cacgacggtg atcatcggcg
      301 gctgaacgtc gagaggtccc gctgcgctat tcttgcgcct cgtccggaaa caaccggagg
      361 tgttgagtgc catacaccca gtccggccgc cggctcaagg tcacgacgcc gctcggcccc
      421 gacgtcctgc tcctgaatgg cttctcggga cacgaagcga tctcgaggcc cttcctgttc
      481 cggctcgagt gcgtcgccga gaacgacaag gccaagcagg tcgtgttcga cgccctgctg
      541 ggccagaagg tcaccgccga gatcgacctc cccggcgggg cgaaacgcca cgtgaacggg
      601 acctgcctgc gtgtcgccga gggcgctcgc gacgccacgt ttacgcgctt cacgctcgac
      661 ctcgtcccgg agccgttcct cctgtcgcgc aggacgcaga gccgcatctt ccagcagatc
      721 acggttcccg acatcctcaa gaaggtcttt gccggcttcg agatggcctg ggagctccag
      781 ggaacgttcg agccgcgcga ctactgcgtg cagtaccgcg agtcggactt cgcgttcgcg
      841 agccgcctga tggaagagga ggggatctac tacttcttca agcacagcga cggcagccac
      901 aagatggtcg tcgcaaacct tccggcgagc cactcggacc ttcccggaaa ggcgaatctg
      961 atcttcgagg ccgtcggggg aggcagccgc gaggaggacc gcgtccacgc ctgggagaag
     1021 tcccaggagc tcaagtcctc gaaggtgaag ttgtgggatc actgcttcga gctgaaccac
     1081 aagcagcatc tcgaggccga gttcccgatc caggctgcgg ttgccgccgg aaaggtgagc
     1141 cacaagctga agctcggcgc caacgcgaag ctcgagctgt acgacttccc cggcgggtac
     1201 gcccagcgtt tcgacggaat ccagcccggc ggcggcgacc gtccggcgga catccagaag
     1261 gtcttcgacg acaacaagcg gaccgtggag ctgcgcatgc aggagcaggc ggccgccagc
     1321 gtcctcgtcc acgccacgag caactgccgc caggtcgtct ccggccacaa gttcacgctc
     1381 gagcgccatt tcgacggcga cggcccgtgg gtcgtctacg aggtcgagca cgcggcgtcc
     1441 gaggcggccg acctgcgttc cggcggaggc ggcttcacct accagaaccg cttcacctgc
     1501 ttcccggtcg ccctcccctt ccggccgccc cgcgtcaccc cgatccccac ggtcaaggga
     1561 acgcagacgg ccgtcgtcgt gggccctccg ggcgaggaga tcttcacgga caagtactcg
     1621 cgtatcaagg tgcagttcca ctgggaccgc gagggcaaga acaacagcga cagctcctgc
     1681 tgggtccgag tcgcgacccc gtgggccgga cagcgctggg gaatgatcca catcccgcgc
     1741 atcggccagg aagtcgtcgt cgacttcctc gagggcgaca tggaccagcc gatcgtcgtc
     1801 ggcagcgtct acaacgccga atgcatgccg ccctacgatc tgcccgcgca caagacgcag
     1861 agcggcatca agagccggag cagcctccag ggaactcccg cgaacttcaa cgagatccgc
     1921 ttcgaggaca agaaggggca cgagcagctc ttcatccacg cggagaagaa ccaggacatc
     1981 gaggtcgaga acgacgaaac gcactgggtc gggcacgacc gcaggaagac gatcgaccac
     2041 gacgagacga cgcacgtcaa gcacgaccgc acggagaccg tcgacaacaa cgagacgatc
     2101 acgatcggga gcaaccggac cgagatggtc ggaatcaacg agacgatcac catcggcgcg
     2161 aaccggacgg agaccgtcgg ggcgaacgag tcgatctcga tcggcgcgaa ccggtccacg
     2221 gcggtcggcg ggagcgagag cgtggcggtc ggcgcgtccc ggaccaccgc catcgggcag
     2281 aacgcggccc tccaggtggg aggtaacgcc acgacccagg tcggaaagaa cgacgagatc
     2341 caggtgggca agagcttcgc gttgatcgcg ggcgaccaga tccagctcca gacgggtgac
     2401 gcaagcatca tcatgaagaa ggacgggacc atcacgatca aggggaagga catcaagctc
     2461 gtggggagcg ggaagatcaa cgtcaaggcg gacggggacg tcgtcatgaa gggaagcaag
     2521 atctcgcaga actgagcggg acgaacagcc atgctccagc tgaagaaccg cacacgcctc
     2581 gccgcgacgc tgttctcgtc gcccgatccg gacggcgtcg aaaccttgta cgcggtcgtc
     2641 aagggcacgt tcaccctcgg cccgcgcgtc gaggaggccg agaagcagac gccgatcgtc
     2701 gtgacggacc gcttccacgg cgaccctctc aagacgagca tcgccgagcc tgccgacatc
     2761 gggctcgtca agcccggcac cgacgtcctc ctgttcggcc acgcctacgg gccgggaggg
     2821 aggccggcgg cgcgagccga ggtgtccttc cgggtcgggc cgatcgagaa gcgcgccgtc
     2881 gtctttggtg atcgcgtgtg gggcggaacg gtcctcacgc gcgtctccgc ccccgagccg
     2941 ttcgaacgga tgccactggt ctgggagcgc gcgttcggag gaacagagcc gcccgcgggc
     3001 gaccctcccc gaaccgacgg cgacgaccgg aacccggtcg gaaccggatt ccgctcgcgc
     3061 atgagcgggc ggaagctcga gggaatcgcc ctgccgaaca tcgaggaccc cgcgaaactc
     3121 atctcttcgc ccggcgaccg gccgtcgccg atgggcttcg gcgccgtcgc gccgcactgg
     3181 aagccgcgcc cgaagtacgc cggaacgtac gacgaggcgt ggcagaagag ccgcgcgccc
     3241 taccttccgc aggacttcga cccgcgcttc ctgcaggtcg cccccgagga ccagcaggtg
     3301 cccggctacc tgagaggcgg agaaccggtg gagatcgccg gcgcgactcc ctccggcgtc
     3361 ctgaggttcg ccctcccggc tcgccgcgtc gacgtcctct tccacctgga ttcaggcgtg
     3421 gaacgacgga ttgcgaacct cgacacggtg ctcctccatc cggacgagtc gcggctcgtc
     3481 ctcgtgtggc gcgccgcact cccctgcgac aagcggctcc tgaaggtccg cgaggtcgag
     3541 ccgatcgtct cgagggcggc gtaggaagac gatggccgcg ccggacgtcg tcgtcgcccg
     3601 cgtcgggatg gtcacggcag tcggactttc tgccgccgag acgatcgcgt ccgttcgctc
     3661 cgcgacgatg cgcttcgaga agatcgactg gctcgaccac cgcttcgagc cgttcacggt
     3721 cgccttcgtc cccgacgccg gcctccccgc gctcgcgccc gacgtcgcaa agacggccgg
     3781 cctcagcatc cgcgacgccc ggctgctccg cctcggcgcg aaggctctcg cggagtgcct
     3841 cggacccgcg cgcctcggcg cgaggctgcg cgcatgagcg cgcgcagcgc gcggctcgcg
     3901 ctgggtggtg cgatcggctg cgcgctgctc gccgccggct gcgtcacgac cagcaacgat
     3961 acgggccagg gctcgccgca ggccgcgggc caggccaacc tgc
//
LOCUS       DAOMFY010000326         4002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8501_length_4002_cov_6.523182, whole genome shotgun sequence.
ACCESSION   DAOMFY010000326 DAOMFY010000000
VERSION     DAOMFY010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8501_length_4002_cov_6.523182"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PLB01_17855"
     CDS             complement(<1..1175)
                     /locus_tag="PLB01_17855"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HQQ79218.1"
                     /translation="MSAGARLSAPDLLRDLASRTLLAVSGKGGVGKTTVASALAALAA
                     RRGRSVLLVSTDGRGDAAALFGREDRGYAEAALGENLRTITTDFDALLTDFVRSVSPV
                     RLLGDRMLAFPAFRFFTRATPGLPDLLLLGKIREVLKRTRSTAKEPRYDLVVLDAPAT
                     GHALSLLALPRTILKTVPAGPLRKLALDVDALSSDPARTALVVVAEPAELAAKETEEL
                     VTGARDAAGVATALVVVNRVGRGGREEILLKRETGVPLIEIQEIDADTSEEDQKISSK
                     GPSPAASGSRGGQVTAVASPLFSAFLATFEETLQSPAPLRAGRSKRPGRAPAPDPLSR
                     SSSLSFFPPNQPRLDLAPWLDGEKLIVLTGPGGVGKTTLSAAVALAAARRGRRALVLT
                     "
     gene            1247..1525
                     /locus_tag="PLB01_17860"
     CDS             1247..1525
                     /locus_tag="PLB01_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79219.1"
                     /translation="MGDSLFDQIRARGESFLTEVSNNLMANPAFIEVLKKGVAAKEEV
                     DKKVVDALRAMNVVTKKDLHRLENRIAELEKEIEALKAKPAKKTARKK"
     gene            1529..2668
                     /locus_tag="PLB01_17865"
     CDS             1529..2668
                     /locus_tag="PLB01_17865"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ79220.1"
                     /translation="MARKVVPGGRVVSFPKASPPPARRTKIAVTGGAGFFGSRLVRSL
                     LLRGEHEVVVFDLVPPADMPGDVRHRFLDLNLPFADGTLYKLLLEEKPDVIVHLAQLR
                     SPSRQTTYAHELNSIGALHVFAAAGEAKVPRVILGSLSLVYGARGDNPNFLTEEHPLR
                     PDPQDRFVQDFVEAEKHAREHVRRHPETAVQILRFVPLLSPEVRDYRARLFASPFSWT
                     LMGYDPLVQALHPDDAVEAILRALDRPEVHGVFNVAPEGILPLSSVRLFFGSLGVPVP
                     HPLAYALYEATWLAGVGALPGVHAHYLRYLCVVSNEKARRVLGFAPRRTTLDCVLETA
                     RVRFGSGRLVDFDALETAARAADFAYERRVHPKRASQRVRQEAAS"
     gene            2665..3663
                     /locus_tag="PLB01_17870"
     CDS             2665..3663
                     /locus_tag="PLB01_17870"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQQ79221.1"
                     /translation="MSARRTVPVKPFVPVSEAPAPSGGFAPSLLPQKQDDLLQALLGE
                     AARFLDAHPEWKGPRAAADLWNLFRHRNRTLSLDDFGYDEAAAKPWHELYDFLYTVWW
                     RVTLAGVENIPSAGPALLVINHSGVLPWDGAMVKRGIMRDHPAHRDARMLILDMFTTL
                     PFLQPWLRHQGEVRACPENGERLLNRGELVGVFPEGVKGVGKLFRDRYRLARFGRGGF
                     VRLALRTGAPIIPVTVVGAEEIYPNLFRMDIIGKPLGLPYFPITPFFPALGPIGVLPL
                     PTKWWIDVGRPIPFNHGPEVADRPMIVNEFADHVRSTLQTMIDSRLSRRGNLFTGE"
     gene            complement(3683..>4002)
                     /locus_tag="PLB01_17875"
     CDS             complement(3683..>4002)
                     /locus_tag="PLB01_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79222.1"
                     /translation="GGPAERAPVRLEARAVLAVSSSGAARIAELKKSAEALPATARTP
                     DVAPFRAKVSADLAAQFKGKSAPAAAATRDALLAEARRRFGSDPKTLAGVEKLITDAS
                     TVR"
BASE COUNT          597 a         1404 c         1419 g          582 t
ORIGIN      
        1 gtgaggacga gtgcgcgccg cccgcgccgc gcggcggcga gcgcgacggc cgcggagagc
       61 gtcgtcttcc cgacgcctcc ggggcccgtc agcacgatga gcttctcccc gtcgagccag
      121 ggggcgaggt cgagacgcgg ctggttcgga gggaagaaag aaagagaaga agatctgctc
      181 agagggtccg gggcgggggc gcggccgggg cgcttcgagc gaccggcgcg caaaggcgcg
      241 ggcgattgaa gagtttcttc gaaggtggcg aggaaggccg agaaaagcgg cgatgccacc
      301 gcagtgacct ggccgcctcg cgatccggag gccgccggtg agggaccttt cgaagaaatc
      361 ttctggtctt cttcggacgt gtccgcatcg atctcctgga tttcgatcag gggaaccccc
      421 gtctccctct tcagcaagat ctcttctctt cctcctcttc cgacccgatt gaccacgacg
      481 agcgccgtcg cgacgcctgc ggcgtcgcgc gcccccgtga cgagctcttc cgtctccttc
      541 gcggcgagct cggcgggctc ggcgacgacg acgagcgcgg tccttgcggg atcggaggag
      601 agcgcgtcga cgtcgagcgc gagcttgcgg agagggccgg ccggcacggt cttgaggatc
      661 gtgcggggga gggcgaggag ggagagcgcg tggcctgtgg ccggcgcgtc gaggacgacg
      721 aggtcgtagc gcggctcctt cgccgtgctg cgcgtccgct tcagcacctc gcggatcttc
      781 ccgagcagga gaaggtcggg caggcccggt gtcgcccggg tgaagaagcg gaacgccggg
      841 aaggcgagca tgcgatcgcc caggaggcgg accggcgaga cggagcggac gaagtccgtg
      901 aggagcgcgt cgaagtcggt cgtgatcgtc cggaggttct ccccgagagc cgcctcggcg
      961 tagccgcggt cctcgcgccc gaagagggcg gcggcatcgc cccgcccgtc cgtggacacg
     1021 aggaggacgg agcggccccg gcgcgccgcg agggcggcaa gcgccgacgc gacggtcgtc
     1081 ttcccgacgc cgcccttgcc cgagacggcg agaagcgtcc gcgaggcgag gtcgcggagg
     1141 aggtccggtg cgctcaggcg ggcccccgcg ctcaccgaaa aagggacata atcggccact
     1201 ctagcaggtg gcgcgccccg agcgcgcccc gcggaaggtg aacgacatgg gcgactcact
     1261 cttcgaccag atccgggcac ggggcgagag ctttctgacc gaggtatcga acaacctgat
     1321 ggcgaacccc gccttcatcg aggtgctcaa gaagggcgtc gcggcgaagg aagaggtcga
     1381 caagaaggtg gtcgacgcgc tccgtgcgat gaacgtcgtc acgaagaagg acctccaccg
     1441 gctcgagaac cgcatcgcgg agctcgaaaa ggagatcgag gccctcaagg cgaagcccgc
     1501 gaagaagacc gcgcggaaga agtaagtcat ggctcgcaag gtcgtacccg gcggacgcgt
     1561 cgtcagcttc ccgaaggcga gcccgcctcc ggcgcggcgg acgaagatcg ccgtgacggg
     1621 cggagccggg ttcttcgggt cgcgtctcgt gcgctcgctc ctgctgcgcg gggaacacga
     1681 ggtcgtcgtg ttcgacctcg tcccgcccgc ggacatgccg ggcgacgtcc gccaccgctt
     1741 tctcgacctg aacctgccgt tcgcggacgg gacgctctac aagctcctcc tcgaggagaa
     1801 acccgacgtc atcgtccatc tcgctcagct tcggagcccc tcgaggcaga cgacctacgc
     1861 tcacgagctg aactcgatcg gcgcccttca cgtcttcgcg gcggcgggcg aggcgaaggt
     1921 cccccgcgtg atcctcggct ccctgtcgct cgtctacggg gcgcgcggcg acaacccgaa
     1981 cttcctgacg gaggagcacc cgctccggcc cgacccgcag gaccggttcg tacaggactt
     2041 cgtggaggcc gagaagcacg cgcgcgagca cgtacgccgg cacccggaga ccgcggtcca
     2101 gatcctgcgg ttcgtaccgc tcctgtcgcc ggaggtccgc gattaccgcg cgcggctgtt
     2161 cgcgtcgccc ttctcgtgga cgctcatggg gtacgacccg ctcgtgcagg cgctgcatcc
     2221 cgacgacgcg gtggaggcga tcctccgcgc gctcgaccgc ccggaagtgc acggcgtgtt
     2281 caacgtggcg cccgagggga tcctgccgct ttcgagcgtc cgcctcttct tcgggtcgct
     2341 cggcgtcccg gtgccgcacc cgctcgccta cgcgctctac gaagcgacgt ggctcgccgg
     2401 cgtcggcgcc ctgccgggag tccacgcgca ctacctccgc tacctgtgcg tcgtgtcgaa
     2461 cgagaaggcg cggcgcgtcc tcggattcgc cccgaggagg acgacgctgg actgcgtact
     2521 cgagaccgct cgtgtgcgct tcggcagcgg ccggctcgtg gacttcgacg cgctcgagac
     2581 ggccgcccgg gccgcggact tcgcgtacga gcggcgcgtc cacccgaagc gcgcgtccca
     2641 gcgcgtccgg caggaggccg cctcgtgagc gcgcgccgca ccgtgcccgt caagccgttc
     2701 gtccccgtgt ccgaggcgcc ggcaccgtcc ggggggttcg cgccctcgct tctcccgcag
     2761 aagcaggacg acctcctgca ggcgctcctc ggcgaagccg cccgcttcct cgacgcgcac
     2821 cccgagtgga aaggcccgcg cgcggccgcg gacctctgga acctcttccg ccaccggaac
     2881 cgaacgctct cgctcgacga cttcggctac gacgaggccg ccgcgaagcc ctggcacgag
     2941 ctgtacgact tcctctacac cgtgtggtgg cgcgtgacgc tcgccggcgt cgagaacatt
     3001 ccgtccgcgg gtcccgcgct cctcgtcatc aaccactcgg gcgttctccc gtgggacggc
     3061 gcgatggtca agcgcggaat catgcgcgac cacccggcgc atcgcgacgc acggatgctc
     3121 atcctcgaca tgttcacgac gctgccgttc ctgcagccgt ggctgcgcca ccagggcgag
     3181 gtccgcgcct gtccggagaa cggggagcgc ctcctgaacc gcggcgagct cgtgggcgtc
     3241 ttccccgagg gcgtcaaggg cgtcggcaag ctctttcgtg accgctaccg gctcgcgcga
     3301 ttcgggcgcg gcgggttcgt gcgcctcgct ctccgcacgg gcgccccgat catccccgta
     3361 acggtcgtcg gcgccgagga gatctacccg aacctcttcc ggatggacat catcgggaag
     3421 cctctcggcc ttccgtattt tccgatcacg ccgttcttcc cggctctcgg cccgatcggc
     3481 gtcctgccgc tgccgacgaa gtggtggatc gacgtgggca ggccgatccc gttcaaccac
     3541 ggacccgagg tcgcggaccg gccgatgatc gtcaacgagt tcgcggacca cgtgcggtcc
     3601 acgctccaga cgatgatcga ctcgcgcctc tcgcggcgcg gaaatctctt cacgggggag
     3661 taggcgcgga gagacgtccc gctcagcgga ccgtcgaggc gtccgtgatg agcttttcga
     3721 cgcccgcgag cgtcttcggg tcggagccga agcgccggcg ggcttcggca aggagcgcgt
     3781 cgcgggtcgc cgccgcggca ggcgcgctct tccccttgaa ctgggcggcg aggtcggcgg
     3841 agaccttcgc gcggaacggc gcaacgtcgg gggtgcgcgc cgtggccggg agcgcctcgg
     3901 ccgacttttt cagctcggcg atccgggcgg cgcccgacga ggagaccgcg aggacggccc
     3961 tcgcctccag gcgcaccggc gcgcgctccg cgggcccgcc cg
//
LOCUS       DAOMFY010000327         3993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8528_length_3993_cov_5.921026, whole genome shotgun sequence.
ACCESSION   DAOMFY010000327 DAOMFY010000000
VERSION     DAOMFY010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8528_length_3993_cov_5.921026"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="PLB01_17880"
     CDS             <1..1480
                     /locus_tag="PLB01_17880"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ79223.1"
                     /translation="GADAAEPGAAAATEKLDTPDSDVRPLPARWAAPLAAPKAVLVKN
                     ATVWTSGPKGTLENASLLVVDGKVAAVGKDVAVPAAAASSLVTIDAQGRSVTPGLIDA
                     HSHTAVDGGVNEGSHNISAEVRIGDVVNPFAPAIYRELAGGLTAANVLHGSSNAIGGQ
                     SEILKLKNGEGPDGLKLRDEPPGIKFALGENPKRSNMQGTGPLRYPQTRMGVSALIRE
                     RFAAARDYRRKQKDFEAAKKRGENPVPVRTDLQLEAIAEILEGRRAIHAHSYVKQEVL
                     DLIRICEEFGVKIGTLQHILEGYKVADEIAAHGAGASSFSDWWAYKFEVYDAIPYNGA
                     LMRERGVLVSFNSDSDELARRMNVEAAKAVKYGGVPREEALKFVTANSAKQLKVDARI
                     GSLEPGKDGDFVVWSGDPLDATSHADETWIEGKKYFDRAEDLKARPAIAAEKTELIGK
                     ARLAASRGAATSPAEPARRPSEHSCSEEEAAMDASAGAEGTR"
     gene            1477..2766
                     /locus_tag="PLB01_17885"
     CDS             1477..2766
                     /locus_tag="PLB01_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ79224.1"
                     /translation="MSTLLLASVLVLRGATVHTATGPAIPNGVVVIDGGRISAVGGAG
                     TPIPAGATVVDAAGKHVAPAFFAPASLVGLVEIPAVRATNDVSEIAEINPEARPDVAM
                     NLDSETLPVTRSNGVLFAALAPRGSTFPGAASVVTLNGWTREDACVKCPAAVLVEWPE
                     MAIDRRADARPSARVQERRRDEALDLIRRTFRDAAAYRAARGAEGKAGVPAHDTVAPF
                     GALGAVLDGSIPLVVRAERKAQIDAALRFRDEDLKGTKARLVILGGYDAPKSAAALAE
                     RKIPVILDTVLELPMREDEPYDAPFTKAAELAKAGVVVAIGNGQTTMAAATTRDLPNH
                     AAMAVSFGLDRLEALRAITLNPARIFGVEARIGSLEAGKDASLAVWTGDPLQATSTVT
                     ALYEKGAALDLSDRHKRLWERYRSRPKPAAPVSAGGR"
     gene            2782..3219
                     /locus_tag="PLB01_17890"
     CDS             2782..3219
                     /locus_tag="PLB01_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79225.1"
                     /translation="MSRGAWIFAGLGLAFIAFLTFVMGKALDTKAPIRIDRAGASSPE
                     PARTPTAEETAARETAEMRAIVAARAPSLARRAADQAFSGEEAARARCTFTGATPVSR
                     DGVSAYWNAEFSCVDPQASGSFPNLTSVSVRLRLDGERWVLDD"
     gene            complement(3226..>3993)
                     /locus_tag="PLB01_17895"
     CDS             complement(3226..>3993)
                     /locus_tag="PLB01_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQQ79226.1"
                     /translation="DRRLPAALLGGGLVAVLAAGVWFAFLRPKAAGPPNAAPVAHDGA
                     TAGAAGKAALAPPPAKPAEEAADPFAAAKKSVVTVVAKLAFDKGFATGTGFFVTPSGK
                     LVTNFHVVKRTDYQQILMPGSKKPIDARILATDEEHDLALLQAMVDPPVPVAPLAAGA
                     LLKMGDPVFALGSPAGPVLEMSLSRGIVSSDKPRQFGDVALIQHDAAINPGNSGGPLL
                     DQQGRVVGVNTLKIKETQGLNFAIPVDQVKDFLSLPR"
BASE COUNT          591 a         1425 c         1457 g          520 t
ORIGIN      
        1 gggcgccgac gccgccgagc cgggcgcggc cgccgccacc gagaagctgg acacgccgga
       61 ctcggacgtg cgcccgcttc cggcgcgctg ggcggccccg ctcgccgcgc cgaaggccgt
      121 cctcgtgaag aacgcgacgg tctggacgtc cggtccgaag gggactctcg agaatgcgag
      181 ccttctcgtc gtggacggca aggtcgccgc ggtggggaag gacgtcgccg tgcccgcggc
      241 cgccgcctcg tcgctcgtca cgatcgacgc gcagggccgg tccgtcacgc ccggcctcat
      301 cgacgcccac agccacacgg ccgtggacgg cggcgtgaac gagggcagcc acaacatctc
      361 ggccgaggtc cggatcggcg acgtcgtgaa cccgttcgcc cccgcgatct atcgcgagct
      421 ggccggcggc ctcaccgccg cgaacgtcct ccacggctcg tccaacgcga tcggcgggca
      481 gtccgagatc ctcaagctga agaacggaga ggggcccgac ggcctcaagc tccgggacga
      541 gccgccgggc atcaagttcg cgctcggcga gaacccgaag cgctccaaca tgcaggggac
      601 cggcccgctc cgttacccgc agacgcgcat gggggtctcg gccctcatcc gcgagcgctt
      661 cgccgcggcg cgcgactacc ggaggaagca gaaggacttc gaggcggcga aaaagcgcgg
      721 cgagaacccc gtgcccgtgc gcacggacct gcagctcgag gcgatcgcgg agatcctcga
      781 gggcaggcgg gcgatccatg cgcacagcta cgtcaagcag gaggtcctcg acctgattcg
      841 catctgcgag gagttcggcg tgaagatcgg gacgctccag cacatcctcg agggctacaa
      901 ggtcgccgac gagatcgcgg cgcacggggc cggcgcctcg tccttcagcg actggtgggc
      961 gtacaagttc gaggtctacg acgcgattcc ctacaacggc gccctcatga gggagcgcgg
     1021 cgtcctcgtc tccttcaact ccgactccga cgagctggcc cgccggatga acgtcgaggc
     1081 cgcgaaggcc gtcaagtacg gcggggtccc gcgcgaggag gcgctcaagt tcgtgacggc
     1141 gaactccgcg aagcagctga aggtcgacgc gcgcatcggc tccctcgagc cgggcaagga
     1201 cggcgacttc gtcgtctggt cgggcgaccc gctggacgcg acgagccacg cggacgagac
     1261 gtggatcgag gggaagaagt acttcgaccg cgccgaagat ctcaaggcgc gtcccgccat
     1321 cgcggcggag aagaccgagc tgatcggcaa ggcgcgcctc gccgcctcgc gcggcgccgc
     1381 gacgtccccc gcggagcccg cgcggcgccc gtccgagcac tcctgcagcg aggaggaagc
     1441 cgccatggac gcatccgccg gggcggaggg gacgcgatga gcacgctcct tctcgcctcc
     1501 gtcctcgtcc tcaggggcgc aaccgtgcac acggcaacag ggcccgccat cccgaacggc
     1561 gtcgtcgtga tcgacggcgg gaggatctcg gcggtcggtg gcgccggcac gccgatcccc
     1621 gcgggagcga cggtcgtcga cgcggcgggc aagcacgtcg cccccgcctt cttcgcgccc
     1681 gcgtcgctcg tcgggctcgt cgagatcccg gccgtgcggg cgacgaacga cgtctccgag
     1741 atcgccgaga tcaaccccga ggcgcggccc gacgtcgcga tgaacctcga ttccgagacc
     1801 cttcccgtca cccggtcgaa cggcgtcctc ttcgccgcgc tcgcgccccg cggctcgacg
     1861 ttccccggcg ccgcctccgt cgtgacgctg aacggctgga cgcgcgagga cgcgtgcgtg
     1921 aagtgcccgg ccgcggtcct cgtcgagtgg cccgagatgg cgatcgaccg gcgcgccgac
     1981 gcccggccgt ccgcgcgggt ccaggagcgc cgccgcgacg aggcgctgga cctcatccgg
     2041 cggacgttcc gggacgccgc ggcgtaccgc gcggcgcgcg gcgccgaggg caaggccggc
     2101 gtgccggcgc acgacaccgt cgcgcccttc ggggcgctcg gggcggtcct cgacgggtcg
     2161 attcccctcg tcgtccgcgc cgagcgaaag gcgcagatcg acgcggcgct gcgcttccgg
     2221 gacgaggacc tcaagggcac gaaggcccgc ctcgtgatcc tcggcgggta cgacgcgccg
     2281 aagtccgcgg ccgcgctcgc cgagcggaag atccccgtca tcctcgacac ggtcctcgag
     2341 ctgcccatgc gcgaggacga gccgtacgac gcgccgttca cgaaggcggc cgagctcgcg
     2401 aaggcggggg tggtcgtcgc gatcggcaac gggcagacga cgatggccgc cgcgacgacg
     2461 cgcgaccttc cgaaccacgc ggcgatggcc gtatcgttcg gcctcgaccg gctcgaggcg
     2521 ctccgcgcga tcacgctcaa tccggcgcgc atcttcggcg tcgaggcgcg gatcggttcg
     2581 ctcgaggcgg gcaaggacgc gtcgctcgcc gtgtggacgg gcgacccgct ccaggcgacc
     2641 tccacggtca ccgcgctcta cgagaagggc gcggcgctcg atctctccga ccgccacaag
     2701 cgcctctggg agcgttaccg gagccggccg aagccggcgg cgcccgtttc ggccggcggc
     2761 cgctgagggg agaagaagag catgagccgc ggcgcctgga tcttcgccgg cctcggactc
     2821 gcgttcatcg cgtttctgac gttcgtgatg gggaaggcgc tcgacacgaa ggcgccgatc
     2881 cggatcgacc gcgccggagc gtcctccccg gagcccgccc ggacgcccac ggccgaagag
     2941 acggctgcgc gcgagacggc cgagatgcgt gcgatcgtcg cagcgcgggc gccttcgctc
     3001 gcccgccgtg cggcggacca ggcgttttcg ggcgaggagg ctgcgcgcgc gcggtgcacg
     3061 ttcacgggcg cgacgcccgt cagccgcgac ggcgtctccg catactggaa cgcggagttc
     3121 tcgtgcgtcg acccccaggc gtccggctcg ttcccgaacc tcacgagcgt gtccgtccgc
     3181 ctccgcctgg acggagagcg ctgggtcctg gacgactgac ggaggtcagc ggggcaggga
     3241 gaggaagtcc ttgacctgat cgacggggat cgcgaagttc agcccctgcg tctccttgat
     3301 cttgagcgtg ttcacgccga cgacgcgacc ctgctgatcg aggaggggcc cgccgctgtt
     3361 gccggggttg atggcggcgt cgtgctggat gagcgcgacg tccccgaact ggcgcggctt
     3421 gtcggaactc acgatgccgc gcgagaggct catctcgagg acggggccgg ccggtgagcc
     3481 gagcgcgaag acgggatcgc ccatcttcag gagcgctccc gccgcgagcg gcgcgacggg
     3541 gaccggcgga tcgaccatcg cctgcagcag cgcgaggtcg tgctcctcgt cggtcgcgag
     3601 gatgcgcgcg tcgatcggtt tcttcgagcc gggcatcagg atctgctggt agtccgtcct
     3661 cttcacgacg tggaagttcg tgacgagctt gccggacggc gtcacgaaga agcccgtccc
     3721 ggtcgcgaat cccttgtcga aggccagctt cgcgacgacc gtgacgacgg atttcttggc
     3781 ggccgcgaac gggtccgcgg cctcttcggc cggcttcgcc gggggtggag cgagagcagc
     3841 tttgcccgcg gctcccgcag tcgccccgtc gtgcgcgacg ggcgcggcgt tcgggggacc
     3901 cgcggctttc ggccggagaa aggcgaacca gactccggcg gcgagcacgg cgacgaggcc
     3961 tccgccgagg agcgcggccg ggaggcgcct gtc
//
LOCUS       DAOMFY010000328         3956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8651_length_3956_cov_8.793386, whole genome shotgun sequence.
ACCESSION   DAOMFY010000328 DAOMFY010000000
VERSION     DAOMFY010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8651_length_3956_cov_8.793386"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLB01_17900"
     CDS             <1..639
                     /locus_tag="PLB01_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HQQ79227.1"
                     /translation="GDPAYTLVQVSVNDLIMLVLFAPIVTLLVSGASNLTVPVKVLIT
                     SVVAFIVIPLVLGSLSRTILLRMKGSEWFEGRFLAFFHPITVIALLATLVLIFAFQAD
                     NILGNWFHVVLIAIPLLIQVYFNSGLAYGLMKLLKVPHDVAAPGALIGASNFFELAVA
                     TAIALYGPGSGAALATVVGVLVEVPVMLSVCSFCNRTRGWFPAASPAEGLAA"
     gene            636..1064
                     /locus_tag="PLB01_17905"
     CDS             636..1064
                     /locus_tag="PLB01_17905"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HQQ79228.1"
                     /translation="MSTDPKLRVLVLCTGNSCRSQMGEGWVRHDLGDRVEVHSAGTRP
                     SVVHPLAIRVMSEAGVDIAPHRSKSVEALKSLAFDLVVTVCDSAREACPILPGARKTL
                     HETFEDPALAGDGEDALPVFRRVRDEIRARLVPVVAAELT"
     gene            1151..2131
                     /locus_tag="PLB01_17910"
     CDS             1151..2131
                     /locus_tag="PLB01_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQQ79229.1"
                     /translation="MAAWVGAYVVNGDVSRFITTRLLGLTPGTHLGAAVQFFVFEVPK
                     VLLLLALVVFVVGIVRSFFTPERTRSLLAGRRETAGNALASLLGVVTPFCSCSAVPMF
                     IGFVTAGVPLGVTFSFLVAAPMVNEIALALLFGLFGWKVAGLYLGTGLVIAFVSGWVI
                     GRLRLEAWVEPWVSRTEAPAAGVAGQRPGWERRLAIGRAQVREIVGKVWPYVVAGIAV
                     GAGIHGWVPENFMASIMGRGAWWSVPLAVLIGIPMYSNAAGILPVVQALLGKGAALGT
                     VLAFMMSVIALSLPEMVILRKVLTPRLIAVFVSVVGTGILLVGFLFNALL"
     gene            complement(2159..2899)
                     /locus_tag="PLB01_17915"
     CDS             complement(2159..2899)
                     /locus_tag="PLB01_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQQ79230.1"
                     /translation="MSPETLVFLAASVLVVAFLYSSVGHAGASGYIAVMSLFSLAPAQ
                     IKPTALVLNVLVASIATWQFWRAGHFSWRLFWPFAVLSIPMAFVGGWIDLPAHAFKAL
                     VGVVLLFSAFRFLFSGSAPDEVAEEPRPLVAIPVGAGLGLLAGLTGTGGGIFLTPILL
                     LKRWARAKSAAAVSALFILVNSVAGLLGNFGSTRRLPSYALVLAAAAVVGGAMGSQLG
                     SRHFRPVAIRRSLALVLLIAGAKLILTR"
     gene            complement(3139..3360)
                     /locus_tag="PLB01_17920"
     CDS             complement(3139..3360)
                     /locus_tag="PLB01_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQQ79231.1"
                     /translation="MKEFFKTNEGTADRILRIVAGIVLLSLTVVGPKTSWGYVGILLV
                     VTGLIGTCPLYSILGLSTCPSKPAGPASR"
     gene            3609..>3956
                     /locus_tag="PLB01_17925"
     CDS             3609..>3956
                     /locus_tag="PLB01_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79232.1"
                     /translation="MVKSAPDRRRTLLFASPLVAALLLAAALVGVSFFAASSLRARDA
                     AVRDGLLSRAGHELEGRLRSDGPEGAERALAEFLAANAALVRGVEVVSFAGVAARAGE
                     TEGAPVEMPAMLGP"
BASE COUNT          564 a         1351 c         1395 g          646 t
ORIGIN      
        1 ggcgacccgg cctacacgct cgtccaggtc tccgtgaacg acctgatcat gctcgtcctg
       61 ttcgcgccga tcgtcacgct cctcgtgagc ggcgcgagca atctgacggt gcccgtcaag
      121 gtcctcatca ccagcgtcgt cgcgttcatc gtcatccccc tggttctcgg gagcctcagc
      181 aggacgatcc tcctccgcat gaaggggagc gagtggttcg agggccgctt cctggccttc
      241 ttccacccga tcacggtgat cgcgctcctc gcgacgctcg tcctcatctt cgccttccag
      301 gccgacaaca tcctgggaaa ctggttccac gtcgtcctga tcgcgatccc gctcctcata
      361 caggtctatt tcaattccgg tctcgcctac gggctcatga agctcctgaa ggtgccgcac
      421 gacgtggcgg cgccgggcgc gctcatcggc gcgagcaact tcttcgagct cgcggtcgcg
      481 accgccatcg ccctctacgg cccgggctcg ggcgcggcgc tcgccacggt cgtcggcgtc
      541 ctcgtcgagg tgcccgtcat gctttccgtc tgctcgttct gcaacaggac gcgtggctgg
      601 ttcccggccg cgagtcccgc ggaaggcctc gcggcgtgag caccgatccg aagctccgcg
      661 tcctcgtgct ttgcacgggc aactcgtgcc ggtcgcagat gggcgagggc tgggtccgcc
      721 acgacctcgg cgaccgcgtc gaggtgcatt cggccggaac gcggccctcc gtcgtgcatc
      781 ccctcgcgat ccgcgtcatg agcgaggccg gggtcgacat cgcgccgcat cgcagcaagt
      841 ccgtcgaggc cttgaagagc ctcgcgttcg acctcgtcgt cactgtctgc gactccgcgc
      901 gcgaagcgtg tccgatcctg ccgggcgcgc ggaagacgct ccacgagacc ttcgaggatc
      961 cggcccttgc cggtgacggc gaggacgcgt tgcccgtgtt ccggcgcgtt cgcgacgaga
     1021 tccgagcccg gctcgttccg gtcgtcgcgg cggagctgac gtgagcgagg ccctttcgct
     1081 tccgacgtac aggcccaagg ccccgccggc cggactgcgg acccggacgg tcctgctcca
     1141 tctcgcggcc ctggccgcct gggtcggcgc ctacgtcgtg aacggagacg tctcgaggtt
     1201 catcacgacc cgtctcctcg gtctgacgcc cgggacgcat ctcggcgcgg cggttcagtt
     1261 cttcgtcttc gaggtcccga aggtccttct tctcctcgcg ctcgtcgtct tcgtcgtcgg
     1321 catcgtgagg agcttcttca cgcccgagag gacgcggagc cttctcgccg ggcggcggga
     1381 gacggccggg aacgcgctcg cgtcgctcct cggcgtcgtc acgccgttct gctcgtgctc
     1441 ggcggtcccg atgttcatcg gcttcgtgac cgccggcgtg ccgctcggcg tgacgttctc
     1501 gttcctcgtg gccgcgccca tggtcaacga gatcgccctc gcgcttctct tcggcctgtt
     1561 tggctggaag gtcgccgggc tctacctcgg aaccggcctc gtgatcgcct tcgtctccgg
     1621 ctgggtcatc ggcaggctga ggctcgaggc gtgggtggag ccgtgggtct ctcgaacgga
     1681 ggcgcccgcc gcgggcgtgg cgggccagcg gccaggctgg gagcgccggc tcgcgatcgg
     1741 acgcgcgcag gtgcgcgaga tcgtcggaaa ggtctggccc tacgtcgtgg ccgggatcgc
     1801 ggtcggcgcc ggaatccacg gctgggtgcc cgagaatttc atggcgtcga tcatgggaag
     1861 gggcgcgtgg tggtccgtgc cgctcgccgt cctgatcggg attccgatgt actcgaacgc
     1921 ggccgggatc ctccccgtcg tgcaggcgct cctcggaaag ggggccgcgc tcggcaccgt
     1981 cctcgcgttc atgatgtcgg tcatcgccct ttcgctcccg gagatggtga tcctccgcaa
     2041 ggttctgact ccgcgcctca tcgcggtctt cgtgagcgtc gtgggaacgg gcattctcct
     2101 cgtcggattc ctcttcaacg cgcttctgtg agagcgccgc cctgcggccg cggggacttc
     2161 agcgggtgag gatcagcttc gcgccggcga tgaggaggac gagcgccagg ctgcgccgga
     2221 tcgcgacggg acggaagtga cggctgccga gctgcgagcc catggccccg ccgacgacgg
     2281 ccgcagcggc gaggacgagg gcgtaggagg ggagtctccg cgtgctgccg aagttgccga
     2341 gcagccccgc cacggagttc acgaggatga agagcgcgga gacggccgcc gcggacttgg
     2401 cgcgcgccca ccttttcagg aggaggatcg gagtgagaaa gatcccgccg cccgtacccg
     2461 tgaggcccgc gagcaggccg aggccggcgc cgaccggaat ggcgacgaga ggccgcggct
     2521 cctcggcgac ttcgtcggga gcggatccgg agaagaggaa gcggaaggcc gaaaacagaa
     2581 ggacgacccc gacgagcgcc ttgaaggcgt gggcgggcag gtcgatccag ccgccgacga
     2641 aggccatggg aatggaaaga acggcgaagg gccagaagag ccgccacgag aagtgccccg
     2701 ccctccagaa ctgccacgtg gcgatggacg cgacgagcac gttcaggacg agcgccgtgg
     2761 gcttgatctg ggcgggcgcg agactgaaga gggacatgac cgcgatgtag ccggaggcgc
     2821 ccgcgtgccc gacggacgaa tagagaaacg ccacgacgag aacggaggcg gcgaggaaga
     2881 cgagcgtttc aggcgacatg cgtgcgcacg accggagccg cgccacgata ccgcgttgcc
     2941 gcgggaggcc cgcggttcag ccggaacggc ggccgaacag gcgctccagc agcggtttcg
     3001 gcggcgggca cgtgcagcgc ttctcgcgcg ggacgccggc aagcgcttcc tcgacgtgcc
     3061 ggccacaacc ggcatacgtc ggcttcccgc accgggaaca gttcacgcga tggcacatga
     3121 caagccctcg cgtgcgcgtc agcgcgaggc cggcccggcc ggtttcgacg ggcaggtgct
     3181 caggccgagg atggaataga gaggacaggt gccgatgagt cccgtcacga cgagcaggat
     3241 gcccacgtag ccccagctcg tcttcggccc gacgaccgtc agcgagagca gtacgatccc
     3301 cgcgacgatc ctcaggatcc tgtccgcggt cccttcgttg gtcttgaaga actccttcac
     3361 ggtgactcct ttcgcgtggc cggctcgtcc ggccccttac gtggcgagca gctcgtcccg
     3421 ccgccggcgg gaccgggcgt ccctcacgtc gggacgccga ggcgggggaa gacgaacttc
     3481 tggaggacga tccagacgag gagggcgccc cacaaccagg cggcgtcact gctcatcccc
     3541 ggctccgggc gcccgcaggg gcggtgttgg gggtggacat gaattacagt ataatcatta
     3601 agttatagat ggtcaagtcc gctcccgacc gccgccgcac cctcctcttc gcctcgcccc
     3661 tcgtcgcggc gctgctgctc gcggccgcgc tcgtgggcgt gtcgttcttc gcggcgagct
     3721 cgctccgggc gcgggacgcc gctgtgcgcg acggtcttct ctcgcgcgcg ggccacgagc
     3781 tggagggtcg cctgcggagc gacggcccgg agggtgccga gcgggcgctc gccgagttcc
     3841 tcgcggcgaa cgcggcgctc gtgcgcgggg tggaggtcgt ctccttcgcg ggtgtcgcgg
     3901 cgcgcgcagg cgagacggag ggcgcgcccg tcgaaatgcc cgcgatgctc gggccg
//
LOCUS       DAOMFY010000329         3955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8657_length_3955_cov_6.245641, whole genome shotgun sequence.
ACCESSION   DAOMFY010000329 DAOMFY010000000
VERSION     DAOMFY010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8657_length_3955_cov_6.245641"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLB01_17930"
     CDS             complement(<1..1122)
                     /locus_tag="PLB01_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79233.1"
                     /translation="MPFRPRELMLPLGLSLVLGAVALRGGPRAVDDAYITFRYARNLV
                     EGAGLVYNPGERVLGTSAPLFALILAGLRSLTGAAIPALAFLVGAACLPACAVAGYRL
                     ARRAAGPLLAGVFVAAALSPHASLEVFASGMETPLYLLGLLFAVELACRGRDTAAFAV
                     ASGLFFLHPDAAALVPALLFAVRAARGRLPWRELALGLAPAAAAAGALAWAYGSPLPH
                     SVTAKRLAYAMPPGHALLRLEETVLDTLVAYATPLAPALVVLLAAGALALLLAFGRRA
                     LTDPPVLAFALFAGAYVAAFALANPLVFPWYRPPLALSTAFVAVACAARLPRPAGAGA
                     SVLLAAAACVHLATFRPYDPSGREAVYQRAAALLALGPAD"
     gene            1185..1733
                     /locus_tag="PLB01_17935"
     CDS             1185..1733
                     /locus_tag="PLB01_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HQQ79234.1"
                     /translation="MLAAARLAGVEARIRLVRGDITALDVDAIVNAANPSLLGGGGVD
                     GAIHRAAGPGLLEECRKLGGCPTGEARITGGHRLKARFVIHTVGPVWHGGSRGEPALL
                     AACYRESLRLARESGCSHVAFPAISCGIYGYPVSDACAIAVAEARAFLAAHVLPERVT
                     FVSFGDEVWEAYRTLLDDGPKG"
     gene            1736..3535
                     /locus_tag="PLB01_17940"
     CDS             1736..3535
                     /locus_tag="PLB01_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQQ79235.1"
                     /translation="MTKSRRDFLAAAATGVAGLAVGARADAQTPPSSTTPAAGTPPAF
                     GTAPPVGPEITPATIAEAEKLMRVTLSPKDRAQAAGNWPAAMAGTMERRTGPRTVVLE
                     PSLAPASQWNPVLPGRSAGPARGVFARSASVPGPLPKRDEDIAFAPVTALSRWIESKA
                     LSSERLTKIYLDRLRRYDPQLKCVITLTPELALEQAQRADAEIAAGRYRGPLHGIPWG
                     AKDLLDTKGIRTTWGAEPYKDRVPDADAYVVKRLHEAGAVLVAKLSLGALALNDVWFG
                     GETKNPWAPEEGSGGSSAGPGAATAAGLVGFAIASETEGSIIDPSMRCGVTGLRPTFG
                     RVARTGAMTLSWSMDKLGPMARGVEDAMLVLHAISGSDPGDVSSVPSRLDFEADGPVA
                     GVKVGYFPGWMKEATDVDRAALGTAKLVGMTPIEVSLPDWPYGSLNTILFAEAAAAFE
                     ELTLSGGLDQLKMQVPDAWPNTFRQARFLSAVDYVQADRLRRKVAGEMTRLFEKVDVL
                     FVPSLRDEMMTISNFTGHPSLTLPAGFVEVGEARSDWAPDPARPLPKFSPRRRVPHGV
                     TLIGRLFDEGTLGRAGVALERAFGVRGERPRGF"
     gene            3580..>3955
                     /locus_tag="PLB01_17945"
     CDS             3580..>3955
                     /locus_tag="PLB01_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HQQ79236.1"
                     /translation="MYDPIAIQPFRDELTRAGFAELRTPGDVDAALAAKGTALVVVNS
                     VCGCAAGQARPGVALAARHPKAPEKLLTVFAGADVEATARARAYFTGYPPSSPSIAIL
                     RDGRIVFMLERREIQTMDARHVA"
BASE COUNT          579 a         1438 c         1485 g          453 t
ORIGIN      
        1 gtccgcgggc ccgagggcga ggagagcggc cgcgcgctgg tagaccgcct cgcggcccga
       61 gggatcgtac ggacggaacg tcgcgaggtg gacgcacgcg gccgccgcga gaaggacgga
      121 cgccccggcg cccgccggcc gcggcagccg ggccgcgcag gcgaccgcca cgaaggccgt
      181 cgagagggcg agcggcgggc ggtaccaggg gaagacgaga gggttcgcga gcgcgaaggc
      241 cgcgacgtag gcgcccgcga agagcgcgaa cgcgaggacg ggcggatccg tgagagcgcg
      301 ccgcccgaac gcgaggagga gcgcgagcgc gcccgcggcg agaagcacga cgagcgcggg
      361 cgccagcggc gtcgcgtacg cgacgagcgt gtcgaggacc gtctcctcga ggcgaaggag
      421 cgcgtggccc gggggcatcg cgtacgcgag gcgcttggcg gtcacgctgt gcggcagcgg
      481 cgagccgtag gcccacgcga gtgctcccgc cgcggccgca gccggcgcga gtccgagcgc
      541 gagctcccgc catggcaacc gcccgcgcgc cgcacgaacg gcgaaaagga gagcgggaac
      601 gagggcggcg gcgtcggggt gcaggaagaa gagcccggaa gcgaccgcga aggccgctgt
      661 gtcccgcccg cggcacgcga gctcgaccgc gaagagaagg ccgagaaggt agagcggcgt
      721 ctccattccg gacgcgaaga cctcgaggga cgcgtgcggg gagagcgcgg cggcgacgaa
      781 cacccccgcg aggagcggcc cggccgcgcg ccgcgcgagg cggtagccgg cgacggcgca
      841 cgccgggagg cacgcggccc cgacgaggaa ggcgagggcc gggatcgcgg cgcccgtgag
      901 agaccggagg cccgcgagga tcagggcgaa gagcggcgcc gacgtgccga gcacgcgctc
      961 cccagggttg tagacgaggc cggcgccctc gacgaggttc cgcgcgtagc gaaaggtgat
     1021 gtaggcgtcg tccacggcgc gcggcccgcc gcggagagcc acggcgccga gcacgagcga
     1081 gagcccgagc gggagcatga gctcgcgcgg gcgaaacggc accgatgcac cggccgtccc
     1141 gggtgccgca agagggggtg cgaggcgcgg agagtcgatg cccgatgtta gcggctgcta
     1201 ggctcgccgg cgtggaagcc cgcatccggc tcgtccgcgg cgacatcacg gcgctcgacg
     1261 tcgacgcgat cgtgaacgcc gcgaatccct cgctcctcgg cggcggcggc gtggacgggg
     1321 caatccaccg ggccgcgggc ccgggcctcc tcgaggagtg ccggaagctc ggaggctgcc
     1381 ccacgggcga ggcgcggatc acgggcggcc accgactgaa ggctcgtttc gtgatccaca
     1441 ccgtgggccc ggtctggcac ggcggcagcc gtggtgagcc ggcgcttctc gccgcgtgct
     1501 accgcgagag cctgcgcctc gcgcgcgaga gcggctgctc gcacgtcgcg tttcccgcga
     1561 tcagctgcgg gatctacggt taccccgtgt ccgatgcctg cgcgatcgcc gtcgccgagg
     1621 cgcgcgcctt cctcgccgcg cacgtcctcc ccgaacgcgt caccttcgtg agcttcgggg
     1681 acgaggtgtg ggaggcttac cgcacgctcc tcgacgacgg accgaaagga tgaccatgac
     1741 gaaatcccgc cgcgatttcc tcgctgccgc cgccaccgga gttgccggcc tcgccgtggg
     1801 cgcgcgggcc gacgcccaga cgccgccgtc ctccaccacc cctgccgccg ggacgcctcc
     1861 ggcgttcggc acggcgcccc cggtggggcc cgagatcacg ccggcgacga tcgccgaggc
     1921 cgagaagctc atgcgcgtca cgctctcgcc gaaggatcgc gcccaggcgg cgggcaactg
     1981 gcccgccgcg atggccggaa cgatggagcg gcgcaccggc ccgaggacgg tcgtcctcga
     2041 gccgtccctc gcgcccgcct cccagtggaa cccggtgctg cccggccgat ccgccggccc
     2101 cgcgcgcggc gtcttcgcgc gcagtgcgtc cgttcccggc ccgctcccga agcgggacga
     2161 ggacatcgcg ttcgcgccgg tgaccgcgct ctcgcgctgg atcgaatcga aggcgctctc
     2221 ttccgagcgg ctgacgaaga tctacctcga ccgcctgcgc cgctacgacc cgcagctcaa
     2281 gtgcgtcatc acgctgacgc ccgaactcgc gctcgagcag gcgcagcgcg cggacgccga
     2341 gatcgcggcc gggaggtacc gcggcccgct gcacgggatt ccgtggggcg cgaaggacct
     2401 cctcgacacg aagggaatcc ggacgacgtg gggcgccgag ccctacaagg accgcgtccc
     2461 cgacgcggac gcgtacgtcg tgaagcgcct ccacgaggcg ggcgccgtcc tcgtcgcgaa
     2521 actgagcctc ggcgcgctcg cgctgaacga cgtctggttc ggcggcgaga cgaagaaccc
     2581 gtgggcgccc gaggagggct cgggcggctc cagcgcgggg ccgggcgccg cgacggccgc
     2641 cgggctcgtc ggcttcgcga tcgcgagcga gacggagggc tccatcatcg acccgtccat
     2701 gcgctgcggc gtcacgggcc tgaggcccac gttcgggcgc gtcgcgcgca cgggcgcgat
     2761 gacgctctcg tggtccatgg acaagctcgg cccgatggcg cgcggcgtgg aggacgccat
     2821 gctggtcctc cacgcgatct ccggttccga ccccggcgac gtttcgagcg tccccagccg
     2881 tctcgacttc gaggcggacg ggcccgtcgc gggcgtgaaa gtcggctact tccccggctg
     2941 gatgaaagag gcgaccgacg tggaccgcgc cgcgctcgga accgcgaagc tcgtcgggat
     3001 gacgcccatc gaggtgagcc tgcccgactg gccgtacgga tcgctgaaca cgatcctctt
     3061 cgccgaggcc gcggcggcgt tcgaggagct cacgctttcg ggcggactcg accagctgaa
     3121 gatgcaggtc ccggatgcct ggccgaacac gttccggcag gcccgcttcc tctcggccgt
     3181 cgactacgtg caggcggacc ggctgcgccg caaggtcgcc ggcgagatga cccgcctgtt
     3241 cgagaaggtg gacgtcctct tcgtcccgtc cctgcgcgac gagatgatga ccatctcgaa
     3301 cttcacgggc cacccctcgc tcacgctgcc ggccggcttc gtcgaggtcg gggaagcgcg
     3361 cagcgactgg gcgccggacc cggcccggcc gctcccgaag ttctcgcccc ggcgccgcgt
     3421 cccgcacggc gtgacgctga tcgggcggct cttcgacgag gggacgctcg gccgcgccgg
     3481 cgtcgccctc gagcgcgcct tcggggtgcg cggagagcgc ccgcgcgggt tctgaaacac
     3541 cacgctgaga aagcgtctca actgaggtat cctccacgca tgtacgaccc catcgccatt
     3601 cagcccttcc gcgacgaact gacccgcgcc ggcttcgccg agctccgcac tcccggcgac
     3661 gtggacgccg ccctggccgc gaagggcacg gcgctcgtcg tcgtgaactc cgtctgcggc
     3721 tgtgcggccg gacaggcgcg ccccggcgtc gcgctcgctg cccgtcaccc gaaggcgccg
     3781 gagaagctcc tcacggtctt cgccggggcg gacgtcgagg ccacggcgcg cgcacgcgcg
     3841 tacttcacgg ggtacccgcc gtcgtcgccc tcgatcgcga tcctccgcga cggcaggatc
     3901 gtcttcatgc tcgagcggcg cgagatccag acgatggacg cgcggcacgt cgccg
//
LOCUS       DAOMFY010000330         3893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8865_length_3893_cov_7.328817, whole genome shotgun sequence.
ACCESSION   DAOMFY010000330 DAOMFY010000000
VERSION     DAOMFY010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8865_length_3893_cov_7.328817"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..982
                     /locus_tag="PLB01_17950"
     CDS             200..982
                     /locus_tag="PLB01_17950"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HQQ79237.1"
                     /translation="MRTTTLSLLALVALGAGSALAQTEAAPVLPACKCPCPCPEPPPP
                     PLTGTLGAGLSITSGNSDTTAFNLAFGLVYDPKTHSLFKADAFWLYSKSDGEATADKA
                     TARLRYEYKFTERIYGFGQVGYLRDRFKNITYLISPMVGAGYLVLKEKTLELGLDGSL
                     GGAFEKDSGFDATSSGAFAVGEAFTWKISPSATFTQKMSGLWKMDDTSDYFFHFDVGV
                     AATLTKNFEMKVSYLVDYKNKPNPSDLKKTDTAFIATILYKF"
     gene            complement(984..1652)
                     /locus_tag="PLB01_17955"
     CDS             complement(984..1652)
                     /locus_tag="PLB01_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HQQ79238.1"
                     /translation="MNSRQLRAVLLAAPFTLLAVSAFGDEPPTVLERVQKKWAENPDM
                     RKLATTQPEALAAVSWLTGTWDAVIKTHATKAEGEKVGKATRTTRFELGGRWIVSRNK
                     GEGPLGEDAVELLGFDPYQRIWRWQFFSSVGRGTNAALVSTRGWDEERLTLAGTFYIW
                     GESADVAIRLVKMSNDEYYEIFEEKLTGELVRPFLEYHYTRAKAAPAVPAPAAKPAPK
                     PPAK"
     gene            1804..3111
                     /gene="aceA"
                     /locus_tag="PLB01_17960"
     CDS             1804..3111
                     /gene="aceA"
                     /locus_tag="PLB01_17960"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HQQ79239.1"
                     /translation="MYRTQSVTELERSWANDLRWKGIVRPYTAQEVDRLRGTVQLDHS
                     IARMGAERLWNLLHTEKFVRALGALTGNQAVQQVQAGLKAIYLSGWQVAGDANDALTM
                     YPDQSLYPVTSVPTVIRKVNNALMRADQIQHMEGKTDIYYFAPIVADAEAGFGGPLNT
                     FELVKSMIEAGAAGIHLEDQLSSLKKCGHMGGKVLVPIHDFIQKLIAARLAADVMGVP
                     TVFIGRTDSLGANLIRGDIDKVDQDFLTGDRTADGFYIVKGGLDYAVARACAFAPYVD
                     LIWCETSTPDIGEAKAFAQGVHERFPGKPLAYNCSPSFNWKRKLSDADIAAFQEELGA
                     MGYKFQFITLAGFHTLNASMYDLAHGYKAQGMTAFVKLQEHEFEMERTAGFQAVKHQR
                     FVGAGYFDQIQMTVTGGEVSTAALKGSTEEEQFYAGAEKKKEH"
     gene            3145..3756
                     /locus_tag="PLB01_17965"
     CDS             3145..3756
                     /locus_tag="PLB01_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79240.1"
                     /translation="MRNAAALLLSLSAAGAALAQQQQAPTPIPQSVVVPSLPDEKNKE
                     GTVRPGATQQTDGGGWVQTNMGQIDPNAPKTGAGTPGTPPPAGSAPGPGIVAPAMPSP
                     PESGPKGKAVVEAANITLRGVVKAYEKGVSVTITEANGKDRTVPLADKAFVYEGVAPG
                     DKVVVRVPLQKPADGKHADRVEKQKAPKAAPASKFSQAQSPKS"
BASE COUNT          669 a         1390 c         1243 g          591 t
ORIGIN      
        1 ccgtccgaga agcggctcgc gcgggccgcg ctcatcgcgg tcctcgtcct cgcggccgcc
       61 gtcgtggtgc tcctcctcgt ccgttgaatt acttccagcc ggacgcagcc gggagttggg
      121 ggtcgatttt cctttcgaag aaggtactct tcgaacggcg gctccgcgag ccgcggacat
      181 ctcgaacaag ggagcttcga tgcgaactac aactctgtcc ttgcttgcgc tcgtcgcgct
      241 cggcgcgggg tccgcgctcg cccagacgga agcggcgccg gtcctgcccg cgtgcaagtg
      301 cccgtgccca tgccccgagc cgccgccgcc cccgctcacg ggcacgctcg gcgccggtct
      361 ctcgatcacg agcggcaact cggacacgac cgcgttcaac ctcgcattcg gactggtgta
      421 cgaccccaag acgcacagcc tcttcaaggc cgacgcgttc tggctctact cgaagtcgga
      481 cggcgaagca acggccgaca aggcgactgc gaggctgcgc tacgagtaca agttcacgga
      541 gaggatctac ggattcgggc aggtcggcta cctgcgtgac cggttcaaga acatcacgta
      601 cctgatttcg ccgatggtcg gcgccggcta cctcgtcctg aaggagaaga ccctcgagct
      661 cggcctcgac ggctcgctcg gcggcgcgtt cgagaaggac agcggcttcg acgcgacgtc
      721 gagcggcgcc ttcgcggtcg gcgaggcgtt cacctggaag atctcgccct cggcgacgtt
      781 cacccagaag atgtccggcc tctggaagat ggacgacacg tccgattact tcttccactt
      841 cgacgtcggc gtcgcggcga cgctcacgaa gaacttcgag atgaaggtct cgtacctcgt
      901 cgattacaag aacaagccga acccgtccga tctcaagaag accgacacgg ccttcatcgc
      961 gacgatcctc tacaagttct aggtcatttc gccggcggct tcggcgcggg cttcgcggcc
     1021 ggcgccggga ccgccggcgc cgccttcgcc cgcgtgtagt ggtactcgag gaacggcctc
     1081 acgagttctc ccgtgagctt ctcctcgaag atctcgtagt actcgtcgtt gctcatcttc
     1141 acgaggcgta tcgcgacgtc cgcggactcg ccccagatgt agaacgtccc cgcgagcgtg
     1201 agccgttcct cgtcccagcc gcgggtcgag acgagggcgg cgttcgtgcc gcggccgacg
     1261 ctcgagaaga actgccagcg ccagatgcgc tggtacgggt cgaatccgag gagttccacg
     1321 gcgtcctcgc cgagcggacc ttcgcccttg ttccgcgaca cgatccagcg gccgccgagc
     1381 tcgaaccgcg tcgtgcgcgt cgccttcccg accttctcgc cctccgcctt cgtcgcgtgg
     1441 gtcttgatca ccgcgtccca cgtgcccgtc agccagctga cggccgcgag ggcctccggc
     1501 tgggtcgtcg cgagcttgcg catgtccggg ttctccgccc acttcttttg cacgcgctcg
     1561 aggacggtcg gcggctcgtc gccgaaggcc gaaacggcga ggagcgtgaa cggcgcggcg
     1621 agcaggacgg cgcggagctg gcggctgttc atggcgacaa ttctattgga ccgtcgaaga
     1681 aacagcctcc aaaaacgtgg aaccccgcgc ccgaaaatcc ctgatgcacc tggctcacct
     1741 gcggtaaatt ccgccggccc cgatgttggg agagacatga tcacgcaagg ggaggaacat
     1801 cccatgtacc gcacgcagtc cgtcaccgag ctcgagcgct cctgggccaa cgacctcagg
     1861 tggaagggaa tcgtccggcc ctacaccgcg caggaggtcg accgccttcg cgggaccgtc
     1921 cagctcgacc actcgatcgc ccggatgggc gccgagcgcc tctggaacct cctccacacc
     1981 gagaagttcg tccgcgcgct cggcgcgctc accggcaacc aggccgtcca gcaggtgcag
     2041 gccggcctca aggcgatcta tctctcgggc tggcaggtcg ccggcgacgc gaatgacgcg
     2101 ctcacgatgt acccggacca gagcctctat ccggtcacgt ccgtcccgac cgtgatccgc
     2161 aaggtgaaca acgccctcat gcgcgcggac cagatccagc acatggaggg gaagaccgac
     2221 atctactact tcgcgccgat cgtcgcggac gccgaggccg gcttcggcgg cccgctcaac
     2281 acgttcgagc tcgtgaagtc gatgatcgag gctggcgcgg ccggcatcca cctcgaggac
     2341 cagctctcgt ccctcaagaa gtgcgggcac atgggcggga aggtcctcgt cccgatccac
     2401 gacttcatcc agaagctcat cgcggcgcgc ctcgcggccg acgtcatggg tgttccgacc
     2461 gtcttcatcg gccggaccga ctccctcggc gcgaacctca tccgcggcga catcgacaag
     2521 gtcgaccagg acttcctcac gggcgaccgc accgccgacg gcttctacat cgtcaagggc
     2581 gggctcgact acgccgtcgc gcgcgcgtgc gcgttcgcgc cgtacgtcga cctcatctgg
     2641 tgcgagacgt cgacgcccga catcggcgag gcgaaggcct tcgcgcaggg cgtccacgaa
     2701 cggttcccgg gcaagccgct cgcgtacaac tgctcgccgt ccttcaactg gaaacgcaag
     2761 ctctccgacg ccgacatcgc cgcgttccag gaggagctcg gcgcgatggg ctacaagttc
     2821 cagttcatca cgctcgccgg cttccacacg ctgaacgcct cgatgtacga cctcgcgcac
     2881 ggctacaagg cgcagggcat gacggccttc gtgaagctcc aggagcacga gttcgagatg
     2941 gagaggaccg cgggcttcca ggccgtcaag caccagcgct tcgtcggcgc ggggtacttc
     3001 gaccagatcc agatgaccgt gaccggcggc gaggtctcca cggcggccct gaagggctcg
     3061 accgaggaag agcagttcta cgccggggcg gagaagaaga aggaacactg acctcgaaac
     3121 gggccgcggc gtaagatttc tcccatgaga aacgccgcgg ccctcctcct ttccctctcg
     3181 gcggcgggcg ccgcactcgc gcagcagcag caggcgccca cgcccatccc gcagagcgtc
     3241 gtcgtgccgt cgctccccga cgagaagaac aaggagggaa cggtccggcc gggcgccacc
     3301 cagcagaccg acggcggcgg ctgggtgcag acgaacatgg gtcagatcga cccgaacgcc
     3361 ccgaagaccg gggccggaac ccccgggacc ccgccgcccg ccggctccgc gcccgggccc
     3421 ggcatcgtcg ctcccgcgat gccgtctcct cccgagagcg gcccgaaggg caaggccgtc
     3481 gtcgaggccg ccaacatcac gctgcgcggg gtcgtcaagg cctacgagaa gggcgtctcg
     3541 gtcacgatca ccgaggcgaa cggcaaggac cgcaccgtgc ctcttgcgga caaggcattc
     3601 gtctacgagg gcgtggcgcc cggcgacaag gtcgtcgtgc gcgtccccct gcaaaagccc
     3661 gccgacggca agcacgcgga ccgagtcgag aagcagaagg cgccgaaagc cgccccggcc
     3721 tcgaagttct cgcaggcgca gtcgccgaag agctgagtct catgggcacc tggactcccc
     3781 cgcccgggca gcccgcgcga cccgcgccgc cttcgcgctt cgacgcgggc cggccggcgc
     3841 cgccgacggg cccgtcggga gggacgatcc tcttcgtcct cgccgcgatc gcg
//
LOCUS       DAOMFY010000331         3879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_8927_length_3879_cov_10.147490, whole genome shotgun sequence.
ACCESSION   DAOMFY010000331 DAOMFY010000000
VERSION     DAOMFY010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8927_length_3879_cov_10.147490"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..780)
                     /locus_tag="PLB01_17970"
     CDS             complement(391..780)
                     /locus_tag="PLB01_17970"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQQ79241.1"
                     /translation="MGAVERLRELGAGREILGEAVPRESDGLRASPRNADLTPEERGA
                     APAVFRALLAGQLLFGLGEKKSAKLLKAVRLALEMEPGLAIEALDVVDAGTLRPVEKI
                     NAPAVLIAAAWAGRTLLADSVRLEPPR"
     gene            complement(783..1445)
                     /locus_tag="PLB01_17975"
     CDS             complement(783..1445)
                     /locus_tag="PLB01_17975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQQ79242.1"
                     /translation="MPAPTPKEFPLRHIAIEGPIGVGKTSLVGLLAKRFSGTQILEDV
                     TNPFLADFYAGKKGAAFQTQMYFLLSRYQQQQEIAQIDLFRNLVVADYTFAKDKIFAY
                     LTLNDAEIALYDKIYATLAANVPRPDLVVHLTASVDTCLSRIKKRGRAFEKGISGEYL
                     KSLVEAYNYFFYHYRDGPLLVVETNEIDFVERKGDLDDLIAQITRPFRGTQIYVPRGS
                     KK"
     gene            complement(1474..2319)
                     /locus_tag="PLB01_17980"
     CDS             complement(1474..2319)
                     /locus_tag="PLB01_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HQQ79243.1"
                     /translation="MKKYLSLVTFAHTVFALPFALLSAVLAAQGLPALPTLLWILVAM
                     VGARSAAMAFNRIADRTLDAENPRTRSRDIPAGRVSLVEASMFCALSAGVFVFAAWRL
                     NPLCLALSPVALVVVLGYSYTKRVTALSHVVLGLGLAIAPVGAWIAVTGAFALLPVVL
                     GLSVLFWVAGFDVIYSLQDEEFDRAHGLFSLPARLGARRALAISTAFHAVSLTLLFLA
                     FVLADGGIPFGLGVVLAGVFLVRQHVLVKPGDLSRVDGAFFTANGWLSVAVFACGAAD
                     VFLRR"
     gene            complement(2334..2960)
                     /locus_tag="PLB01_17985"
     CDS             complement(2334..2960)
                     /locus_tag="PLB01_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HQQ79244.1"
                     /translation="MARQAGGKNDLILGISGASGARLGMRALRLFSASPDVERLHVVV
                     SPRALVVARDEVDAGITSVEALLDAAELDAASRGKLVLHPESAIDASISSGSYKTRGM
                     AVLPCSAGTLASIAHGTSRGLLQRAADVCLKERRRLVLGLRETPLSLVHAENILAVTR
                     AGAIVAPPVPAFYAARTAEAMLDAYLTRVADLLDVAVDTAGFRWTGAR"
     gene            3325..3741
                     /locus_tag="PLB01_17990"
     CDS             3325..3741
                     /locus_tag="PLB01_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQQ79245.1"
                     /translation="MTLRPIVRALVVPVLGLSLSGIASHPALAEGEAAPAAHKVVNIN
                     QASAEEIARLPRVGPKLAGRIVEHRSQHGAYKRPEDLMQVKGVGEKFFTTLKPYVAVS
                     GATTLAEKVSSKGSRGGSKSAKTASSGTKKPAKTKA"
BASE COUNT          673 a         1289 c         1387 g          530 t
ORIGIN      
        1 tcgttcgccg agaagatgaa gggggcgccg cagtcgatac aggggatgga tttgtccgtg
       61 tacatcagaa ctccagaggg ggtcgccggc tctaaccgga aaccgagggt tggaaatcgc
      121 ggacgacgct caccatcgag cggcggacgc cgggaactgg cgaggggtgc cggtcggaga
      181 gttcaggaag gagggacggg gctgtgttcc tgctgctgcc cccggggctg gaaatgggtc
      241 tcgcctcctt cgaactcgat ccgacgaaca gactgacacg gccttcgaag gcattcgggg
      301 ctgacccttg ggctctccgg ggctgttggt aggggaatga gaatcgaaaa aatcaacgaa
      361 atcaagcgaa aaagaaattg cgacgtggta tcagcggggc ggctcgaggc gcacgctgtc
      421 ggcgaggagc gtgcggccag cccaggccgc cgcgatcagg accgccgggg cgttgatttt
      481 ctcgacaggc cggagcgtac ccgcgtcgac gacgtccagc gcctcgatcg cgagaccggg
      541 ctccatttcg agcgcgagcc gcaccgcctt gaggagcttc gcggacttct tctcgccgag
      601 gccgaagagg agctggcccg cgagaagcgc gcggaagacc gccggcgcgg cgccgcgctc
      661 ctccggcgtg aggtccgcgt tccgggggga cgcgcgcagg ccgtcgctct cccgcggcac
      721 ggcctcgccg aggatctcgc gccccgcacc cagctcgcgc agccgctcga cggcgcccac
      781 gcctacttct tcgagccgcg cggcacgtag atctgcgtcc cgcggaacgg gcgcgtgatc
      841 tgcgcgatga ggtcgtcgag gtcgcccttc ctctcgacga agtcgatctc gttcgtctcg
      901 acgacgagca gcgggccgtc gcggtagtgg tagaagaagt agttgtaggc ctcgacgagc
      961 gacttgaggt actcgcccga gatgcccttc tcgaacgcgc gtccgcgctt cttgatgcgc
     1021 gaaaggcacg tgtccacgga cgccgtgagg tggacgacga ggtccgggcg cgggacgttc
     1081 gcggcgagcg tcgcgtagat cttgtcgtag agcgcgatct ccgcgtcgtt cagcgtgagg
     1141 tacgcgaaga tcttgtcctt cgcgaacgtg tagtccgcga cgaccaggtt ccggaaaagg
     1201 tcgatctggg cgatttcctg ctgctgctgg tagcgcgaga gcaggaagta catctgcgtc
     1261 tggaacgccg cgcccttctt gcccgcgtag aaatccgcga ggaaggggtt cgtcacgtcc
     1321 tcgaggatct gcgttccgct gaagcgcttc gcgagcagcc cgacgagcga ggtcttcccg
     1381 acgccgatcg gtccctcgat cgcgatgtgg cggagcggaa actccttcgg ggtgggcgcc
     1441 ggcacgaggt gccgagttta ccggtccgcg aggctaccgg cggaggaaga cgtcggccgc
     1501 cccgcaggcg aagacggcca ccgagagcca gccgttggcc gtgaagaacg ccccgtccac
     1561 ccgggagagg tcgcccggct tgacgaggac gtgctgccgc acgaggaaga ccccggccag
     1621 gacgacgccg agcccgaacg ggatcccgcc gtccgcgagg acgaaggcga ggaagaggag
     1681 cgtcagggag acggcgtgga aggcggtgga gatcgcgagc gcccgtcgcg cgcccagccg
     1741 cgcggggagc gagaacaggc cgtgcgcgcg gtcgaactcc tcgtcctgaa ggctgtagat
     1801 gacgtcgaag cccgcgaccc agaagaggac cgagaggccg aggacgaccg ggagcagggc
     1861 gaaggcgccc gtgaccgcga tccaggcccc gacgggcgca atcgcgaggc cgaggccgag
     1921 gacgacgtgc gagagcgccg tgacgcgctt cgtgtacgag tagccgagga cgacgacgag
     1981 cgcgacgggc gagagcgcga ggcagagcgg gttgagccgc cacgccgcga agacgaagac
     2041 gcccgccgaa agcgcgcaga acatcgaggc ctcgacgagg gagacgcgtc cggccgggat
     2101 gtcccggctc ctcgtccgcg ggttctccgc gtcgagggtg cggtcggcga tccggttgaa
     2161 ggccatcgcg gccgagcggg cgcccaccat cgcgacgagg atccagagaa gcgtcgggag
     2221 cgccggcagg ccctgcgccg cgaggacggc cgagagcagc gcgaacggga gcgcgaagac
     2281 cgtgtgcgcg aacgtgacga gggagaggta cttcttcacg gggccgagag gtctcagcgc
     2341 gctccggtcc agcggaagcc ggccgtgtcg accgcaacgt cgaggaggtc cgcgacgcgc
     2401 gtgaggtagg cgtcgagcat ggcctcggcc gtgcgcgccg cgtagaacgc ggggacgggc
     2461 ggcgcgacga tcgcgcccgc gcgcgtgacg gccaggatgt tctcggcgtg gacgagggag
     2521 agcggcgtct ccctcaggcc gaggacgagg cgccggcgtt ccttcaggca gacgtcggcc
     2581 gcgcgctgca ggagcccgcg cgacgtcccg tgcgcgatgg aggcgagcgt gcccgccgag
     2641 cacggcagga ccgccatccc gcgcgtcttg tacgagcccg acgaaatcga ggcgtcgatc
     2701 gcgctctcgg ggtggaggac gagtttgccc cgcgacgcgg cgtcgagctc cgccgcgtcg
     2761 agcagcgcct cgacggacgt gatccccgcg tccacctcgt cgcgcgcgac gacgagggcc
     2821 cgcggagaga cgacgacgtg gaggcgctcg acgtccggcg acgccgagaa gagccgcagc
     2881 gcccgcatcc cgagccgcgc gccgctggcc ccggagatcc cgaggatgag gtcgttcttc
     2941 ccgcccgcct gccgcgccat cgcccgattc tccaccacga cagcttttcc tgtcgcgctc
     3001 tccctttccg tgcaggagaa gagaagagat tttcttagaa ggtaataggg caaacggcgt
     3061 cggggcgcgc gacaggagat gttgtcgcgc accgcctccg ccctcttccc ctctctaagc
     3121 agaattctct tctcttcaat cgtttgcgtg catcgcccgc cgtccggcat cgcccgtgcg
     3181 atggggtccg gcgaggtgat cgatgggtta ggcgccacac acgcgcaccc ggacgcgccg
     3241 ggacgaagcg gaaggaacaa acccccgaat cccggttcgt gtcccttttc cgaacaaaga
     3301 agaaagaaaa caaaggagtt cacgatgact ctccgtccga tcgtccgcgc cctcgtcgtc
     3361 cccgtcctcg gcctttcgct ctccggaatc gcgtcgcacc cggctctggc cgagggcgaa
     3421 gcggcgccgg ccgcgcacaa ggtcgtcaac atcaaccagg cctccgccga ggagatcgcg
     3481 cgcctgccgc gcgtcggccc gaagctggcc ggccggatcg tcgagcaccg cagccagcac
     3541 ggcgcgtaca agcgccccga ggacctcatg caggtgaagg gcgtcggcga gaagttcttc
     3601 acgacgctca agccctacgt cgccgtctcc ggcgcgacga ccctcgccga gaaggtgtct
     3661 tcgaagggct cgcgcggcgg ctcgaagagc gccaagaccg cctcctccgg gacgaagaag
     3721 cctgccaaga cgaaggcctg agtccctccc cccctccggg acggcgcggc gctccgccgc
     3781 gccgtccccc ctcctcacgc gcacgaaggg aggaaccatg cgcgcatccg aacccggagt
     3841 cccgctggcc gagctcgccg tcgtcatcgc catcctcgg
//
LOCUS       DAOMFY010000332         3858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9006_length_3858_cov_5.455693, whole genome shotgun sequence.
ACCESSION   DAOMFY010000332 DAOMFY010000000
VERSION     DAOMFY010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9006_length_3858_cov_5.455693"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /gene="rfaE2"
                     /locus_tag="PLB01_17995"
     CDS             <1..495
                     /gene="rfaE2"
                     /locus_tag="PLB01_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HQQ79246.1"
                     /translation="EGLVASPHEKVLGRAHVASVSAVLRRAHKRVVFTNGCFDLLHPG
                     HVRLLAEARAHGDVLVVGINSDASVKRLKGEGRPILSESDRAQVLGGLDAVDFVVVFD
                     EDTPQSLIEEIRPQVLVKGGDYVEGTVVGAPFVRSTGGSVVLVPLVEGRSTSSMVARM
                     KGDA"
     gene            495..1058
                     /locus_tag="PLB01_18000"
     CDS             495..1058
                     /locus_tag="PLB01_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQQ79247.1"
                     /translation="MRAYFADLAALASGAEASIGAGVAKASRLVADAALAGKTTFAFG
                     NGGSATQAQHFAAELVVRYKDDRPALPAIALVSDIAILTACTNDYDYSVVFSRQIEAL
                     AKAGDVAVGLTTSGRSPNVLKALAAARARGLSTVFLTGEKGRAEAANWDVGIVVPSTE
                     TAHVQELHLAALHLMCRFVDEERARRA"
     gene            1060..1704
                     /locus_tag="PLB01_18005"
     CDS             1060..1704
                     /locus_tag="PLB01_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79248.1"
                     /translation="MGGRAAAVLKAAAIALVALFAVDRFLPGVPLALLSSEGAPLPNA
                     LKEPVQAEAGREQFSETRGRRTFRITPRAAYDVAARVGATERYRWGASGALIPWDFVL
                     TWGAATREPAWSRISYLQTGRFYNWRCDDSLDPSYVSSHTANTHLIPASSRIASALAH
                     VRRGDVVRLEGDLVDVDGPDGFLWKTSLTRTDTGPGACETLYVRAVTIGTRRYR"
     gene            complement(1701..2732)
                     /gene="waaF"
                     /locus_tag="PLB01_18010"
     CDS             complement(1701..2732)
                     /gene="waaF"
                     /locus_tag="PLB01_18010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HQQ79249.1"
                     /translation="MKTLVRATNWVGDVVMSRPALRALKAADPSGRLAVLARPWVAEL
                     YRLAEEVDDVLVDDASGRHEGSAGRARLADEVREAGFDRAVVLPTSFSTALVLARAGV
                     PERIGYRGEGRGPLLTRAVRLDLAAGEHQVWKHLRLAEAAGASVPAAPDVSWDAPKAV
                     LQAARNRLAEAGVAGPFVAAHVASFAHAAKRWDLARFAAVFDGLSSRGLPVVLLGSAG
                     EKSVNAEAAAFTRTARAVDLSGTTTLPEALGVLASARLFVGNDSGLAHLASAAGTPSV
                     VVFGPTDPDATRPWDGPRADGRPVRLALVRRRTPCAPCGWAVCPIDHACMAAVAPSDV
                     LAAAESVLS"
     gene            complement(2729..>3858)
                     /locus_tag="PLB01_18015"
     CDS             complement(2729..>3858)
                     /locus_tag="PLB01_18015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ79250.1"
                     /translation="FNMGVPTYAAMAATVAFTVVHSLVGLSAKARAAAGVATSRRPLV
                     LAGLAARGHSVRLLARRGSPLASEARARGLDTVEVPAGPEASPALLLAVARAARTFRP
                     EIVYAGDARGHGAAVFGRAAADAPLVVHRRVVFAPGRSPISRLKYRAAARYLAVSHAV
                     AASLGDAGIPSGKIALVPDGLPPAAFRENVAPPPPPFRLVHAGAFDGLKGQDVVVETL
                     ARLAAGGIDAHALFLGAGPARAGVEALAASRGVADRCTFAGQVEDAGERFAASHLMLL
                     PSASEGGPLVLVEAMAAGCPVVGHDVGGSREMVADGAAGVLVPTLELSAWEEAVRGIL
                     LDAARRARLVEAGRAAAAERRIEKTVARVEAELLRTLGETP"
BASE COUNT          522 a         1400 c         1463 g          473 t
ORIGIN      
        1 gagggcctcg tcgcctcgcc gcacgagaaa gtgctcgggc gcgcgcacgt ggcgtccgtt
       61 tcggccgtgc tccgtcgggc gcacaagcgc gtcgtcttca cgaacggctg cttcgacctg
      121 ctccatccgg ggcacgtcag gctcctcgcg gaggcgcgcg cgcacggtga cgtcctcgtc
      181 gtcggcatca acagcgacgc ctccgtgaag aggctcaagg gcgaggggag gcccatcctc
      241 tcggagtccg accgcgccca ggtcctcggc ggcctcgacg ccgtggactt cgtggtcgtc
      301 ttcgacgagg acacgccgca gtccctcatc gaggagatcc ggccgcaggt tctcgtgaaa
      361 ggcggcgact acgtggaagg gacggtcgtc ggcgcgccgt tcgtcaggtc gacgggcggg
      421 agcgtcgtcc tcgtcccgct cgtggaaggc cgttcgacct cgtcgatggt cgcgcggatg
      481 aagggagacg cctgatgcgc gcgtacttcg cggaccttgc ggccctggca tcgggcgccg
      541 aggcctcgat cggcgccggc gtcgcgaagg cgtcccggct cgtcgcggac gcggcgctcg
      601 ccgggaagac gacgttcgcg ttcggcaacg gcggcagcgc cacgcaggcg cagcacttcg
      661 cggccgagct cgtcgtgcgc tacaaggacg accggcccgc gcttcccgcg atcgcgctcg
      721 tctcggacat cgcgatcctg accgcctgca cgaacgacta cgactactcc gtcgtgttct
      781 cgcgccagat cgaggcgctc gcgaaggcgg gcgacgtggc cgtgggcctc acgacgtccg
      841 ggaggagccc gaacgtcctc aaggctctcg ccgccgcgag ggcgcgcggc ctgtcgaccg
      901 ttttcctcac gggagagaag ggccgtgcgg aggccgcaaa ctgggacgtc ggcatcgtcg
      961 tgccctcgac cgaaacggcc cacgtgcagg agctgcacct cgccgcgctc cacctgatgt
     1021 gccgtttcgt ggacgaggag cgggcccgac gcgcctgacg tgggcggacg cgcggcggcc
     1081 gtcctgaagg cggcggcgat cgccctcgtc gcactgttcg ccgtcgaccg gttcctgccc
     1141 ggcgtcccgc tcgcgctcct gtcttccgag ggcgccccgc tcccgaacgc gctcaaggag
     1201 cccgtgcagg ccgaggcggg ccgggaacag ttttccgaga cgcgggggcg gcggacgttc
     1261 cggatcacgc cgcgggccgc gtacgacgtc gcggcgcgcg tgggtgcgac ggagcgctac
     1321 cgatggggcg cgtcgggcgc gctcatcccg tgggacttcg tcctgacctg gggcgccgcc
     1381 acacgggagc ctgcctggtc gcgcatctcg tacctgcaga cggggcggtt ctacaactgg
     1441 cgctgcgacg actcgctgga tccgtcgtac gtctcgtcgc acacggcgaa cacgcatctc
     1501 attccggcga gcagccgcat cgcgtcggcg ctcgcgcacg tgaggcgcgg cgacgtcgtg
     1561 cggctggagg gcgatctcgt ggacgtggac ggcccggacg gcttcctctg gaagacgagc
     1621 ctcacgcgga ccgacacggg tccgggcgcc tgcgagacgc tctacgtgcg cgcggtgacg
     1681 atcgggacgc gccgctaccg ctaggacagg accgactccg ccgcggcgag gacgtcggac
     1741 ggcgccacgg ctgccatgca tgcatggtcg atcgggcaga ccgcccatcc gcacggcgcg
     1801 cacggagtcc gccgccgcac gagagcgagc cggacgggcc tgccgtcggc gcgcggcccg
     1861 tcccacgggc gcgtcgcgtc gggatcggtc gggccgaaca ccacgacgga gggagtcccg
     1921 gccgcgctcg cgaggtgcgc gaggcccgag tcgtttccga cgaagaggcg cgcggacgcg
     1981 aggacgccga gagcctcggg gagcgtcgtc gttcccgaga ggtcgacggc gcgcgccgtc
     2041 cgggtgaacg ccgccgcctc cgcgttgacg ctcttctcgc ccgcgctccc gagcaggacg
     2101 acgggaagcc cgcgggagga caggccgtca aagaccgcag cgaaacgcgc gaggtcccag
     2161 cgcttggccg cgtgcgcgaa cgaggccacg tgtgcggcga cgaagggtcc ggcgaccccg
     2221 gcctcggcga ggcgattgcg ggccgcctgg aggacggcct tcggcgcgtc ccacgagacg
     2281 tccggagccg cgggaaccga tgcccccgcg gcctcggcga ggcgcaggtg cttccagacc
     2341 tggtgctcgc cggccgcgag gtccagccgg acggcgcgcg tgagcagcgg gccgcggccc
     2401 tcgccccggt acccgatgcg ctcggggacg cctgcgcgcg cgaggacgag cgcggtcgaa
     2461 aagctcgtcg ggagaacgac ggcccgatcg aagccggcct cgcgcacctc gtccgcgagc
     2521 cgcgcgcgtc ccgcgcttcc ctcgtggcgc ccggaggcgt cgtcgacgag gacgtcgtcg
     2581 acctcttcgg cgaggcggta cagctccgcg acccacgggc gcgcgaggac cgcgagccgt
     2641 cccgaagggt ccgcggcctt gagcgcgcga agcgcggggc gcgacatgac gacgtcgccg
     2701 acccagttcg tcgcgcgcac gagcgtcttc acggcgtctc tcccagggtc cggaggagct
     2761 cggcctcgac ccgcgcgacc gtcttctcga tcctccgctc ggcggcggcg gcgcgcccgg
     2821 cctcgacgag gcgcgcccgg cgcgccgcgt cgaggaggat gccgcgcacg gcctcctccc
     2881 aggcggagag ctcgagcgtc ggcacgagga cgcccgcggc gccgtccgcg accatctcgc
     2941 gcgaacctcc gacgtcgtgc ccgacgaccg ggcatccggc cgccatcgct tcgacgagga
     3001 cgagcggtcc gccctcgctc gccgagggca ggagcatgag atggctcgcc gcgaagcgct
     3061 ctcctgcgtc ctccacctgg cccgcgaacg tgcagcggtc cgcgacgccg cgggacgcgg
     3121 cgagcgcttc gacgccggcg cgcgcgggtc cggcgccgag gaagagcgcg tgggcgtcga
     3181 tccctcccgc ggcgagccgt gcgagcgtct cgacgacgac gtcctgaccc ttgagcccgt
     3241 cgaacgcgcc cgcgtggacg agccggaacg gcggaggcgg aggcgcgacg ttctcccgga
     3301 acgcggcggg gggaagcccg tcggggacga gggcgatctt cccggacgga atgccggcgt
     3361 ctccgaggga ggccgcgacg gcgtgggaga cggcgaggta ccgggcggcc gcgcggtatt
     3421 tgaggcgcga gatcgggctc cgtcccggtg cgaagacgac gcggcggtgg acgacgaggg
     3481 gcgcatccgc cgcggcgcgg ccgaacaccg cggcgccgtg gccgcgcgcg tctccggcgt
     3541 agacgatttc gggccggaag gtccgcgccg cgcgcgcgac cgcgaggaga agcgccggag
     3601 acgcctccgg gccggcgggc acttcgaccg tgtcgaggcc gcgtgcgcgg gcttcggacg
     3661 ccaggggact cccgcggcgg gcgagaaggc ggacggagtg gccgcgcgca gcgaggccgg
     3721 ccaggacgag cggccggcga gaagttgcca cgcccgccgc cgcccgcgcc ttcgcgctca
     3781 atccaaccag cgaatggacg accgtgaacg ctaccgtcgc cgccatcgcc gcgtacgtcg
     3841 gcacgcccat gttgaacg
//
LOCUS       DAOMFY010000333         3817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9181_length_3817_cov_7.307815, whole genome shotgun sequence.
ACCESSION   DAOMFY010000333 DAOMFY010000000
VERSION     DAOMFY010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9181_length_3817_cov_7.307815"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLB01_18020"
     CDS             complement(<1..401)
                     /locus_tag="PLB01_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79251.1"
                     /translation="MKATSPGFLARRVGVSLIAVAALLLGACTCTTPPPFAITAPVNG
                     DTLSAEACKAVTVSVTPIAGDYCTFPPKAYEISVDGGAPQTIPADKTPLSATFNNLGT
                     GPHTATAWAIGQNGKRRETASVKFTCAPPPPP"
     gene            complement(416..1927)
                     /locus_tag="PLB01_18025"
     CDS             complement(416..1927)
                     /locus_tag="PLB01_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79252.1"
                     /translation="MKTPHTLLRLLSAAAVAAVLALPAAAQVDLSRLVWIGDSEGAGY
                     SANCLTKRVQVDSPGAVIARANGVDYQQPILSDWGTGGCLVLTSLAPSFGTCPGNAAC
                     PPPTTLGVPENLALPRPYNNLAIGGCAIHEMLYATTAADVASTGACKSTIDLVLRNSV
                     LKIGSQVDQAIALNPSFVILANFGNDYLGAVLSGTTVDGVTVTPLAAFTADTNAALAK
                     LSARQKNGIVTSVGNLTNFPFTTTLPPYVTSGGKVVIVNGAPIPLLGPKGCPTGVPAC
                     PIPSNTIVTLNAAGYLPLGFGIPCAVAPTLPKCNNPLPDSQSAAGPGVLLYADEVALL
                     TQRIADYNAAIKAAAATYGYKFYDINDWLVRTKAGFDYGGATVSQAFLSGGIASYDGF
                     HLTPIGYALLADDIIKFINANFPGANLKEPDLSTYLYAGGTAGGLSGPFTAPYAWAPM
                     NDAEKATAIEQIFTLDFASDLAAMIGPRRASVTPGPSAPVQRHERADRTDPMP"
     gene            complement(1944..3245)
                     /locus_tag="PLB01_18030"
     CDS             complement(1944..3245)
                     /locus_tag="PLB01_18030"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQQ79253.1"
                     /translation="MFKAIGTRLAALVLVGLAGALPAARLEAAGFALFEQSSKGNAMA
                     GAFTATAEDPSAMFYNPAGNAFIDETIVDGSIFGVLRPTARLDGLNPFPGEGYSANMH
                     KPLYWFGNMYFVTSLSKDFKVSLGMWSPNGLGVPWQNPDTFRGRVINQRTDLRQLAVS
                     AQFAWKVSDAIAIGGGPEVRFTDVKLSRNVQAVNPFTQRVQDIAHLSLISTGTPIKVT
                     WTAGMLIKPCDRLRFGISYHAHVDVDLEGPAQFSQIATGSPQFDAAVSTLLPFGTNPT
                     GRTTLQFPSLTAFGVAYDVTPNLTLAVDGNYVTWKVFDQTVIRIEGLPDSVLPHYWEN
                     TWAVRVGALYKGKKGWLGAGFVYDQTPQPDADASPFLPDNNRTGVTIGAGFNVLHGVQ
                     LNVSSLFLWFHERTITTNNDNFSATYKTFAILPSVGMKTTF"
     gene            complement(3382..>3817)
                     /locus_tag="PLB01_18035"
     CDS             complement(3382..>3817)
                     /locus_tag="PLB01_18035"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQQ79254.1"
                     /translation="AEVVRTAEDAADRVRLFAAVPSLDSLVRGGRVTAGQRRLANLLG
                     VVPLLTLDAAGRARAGGAARGFPRACVRLVEKSLKAAAGVSSPVFAVAHADAAELAER
                     IARELLARRPGAEEYVVEVTPVLAAHAGPGAVAVATLGARPR"
BASE COUNT          568 a         1224 c         1428 g          597 t
ORIGIN      
        1 gggggcggcg gcggcgcgca cgtgaacttc acggacgcgg tctcgcggcg cttgccgttc
       61 tgcccgatcg cccacgcggt ggccgtgtgc gggccggtcc cgaggttgtt gaacgtcgcg
      121 gagagcggcg tcttgtccgc cgggatcgtc tgcggcgcgc cgccgtcgac cgagatctcg
      181 taggccttcg gcgggaacgt gcagtagtcg cccgcgatcg gcgtgacgga cacggtgacg
      241 gccttgcagg cctccgcgga cagcgtgtcg ccgttcacgg gcgccgtgat cgcgaacggc
      301 ggcggggtcg tgcaggtgca cgcgccgagg agcagcgcgg ccacggcgat caggctgacg
      361 ccgacgcgcc gagcgaggaa tccgggagag gtcgctttca cgtcaatcct cctgttcagg
      421 gcatcgggtc ggtgcggtcc gcgcgctcgt ggcgctgcac cggcgcggag ggtccgggcg
      481 tcacggaggc gcggcgcggc ccgatcatgg ctgcgagatc gctggcgaaa tcgagcgtga
      541 agatctgctc gatcgccgtc gccttctccg cgtcgttcat gggagcccag gcgtaaggcg
      601 cggtgaacgg gcccgagagc ccgccggccg tgccgcccgc gtagaggtac gtggagaggt
      661 ccggctcctt gaggttcgcg cccgggaagt tcgcgttgat gaacttgatg atgtcgtcgg
      721 ccagcagggc gtagccgatc ggcgtcaggt ggaagccgtc gtacgaggcg atgccgcccg
      781 acaggaaggc ctggctcacc gtcgcgccgc cgtagtcgaa gccggccttc gtgcgcacga
      841 gccagtcgtt gatgtcgtag aacttgtacc cgtacgttgc ggccgccgcc ttgatcgccg
      901 cgttgtagtc ggcgatgcgc tgcgtgagga gcgcaacctc gtccgcgtag agaaggacgc
      961 ccgggccggc ggcgctctgg gaatccggga gcgggttgtt gcacttcgga agcgtcggcg
     1021 ccaccgcgca cgggattccg aagccgagcg gcaggtagcc cgctgcgttg agcgtcacga
     1081 tcgtgttcga cggaatcggg caggccggga cgcccgtggg gcagcccttc ggcccgagga
     1141 gcgggatggg cgcgccgttc acaatgacga ccttgccgcc gctcgtgacg tacggcggga
     1201 gcgtcgtcgt gaacgggaag ttggtcaggt tgccgacgct cgtgacgatg ccgttcttct
     1261 gccgggccga gagcttcgcg agcgcggcgt tcgtgtcggc cgtgaacgcg gcgagcggcg
     1321 ttaccgtgac gccgtcgacc gtcgtcccgg agaggaccgc gccgaggtag tcgttgccga
     1381 agttcgcgag gatcacgaac gacgggttga gggcgatcgc ctggtcgacc tgcgacccga
     1441 tcttcagaac cgaattcctc aggacgaggt cgatcgtgct cttgcacgcg cccgtggaag
     1501 ccacgtcggc cgccgtcgtg gcgtagagca tctcgtggat cgcacagccg ccgatcgcga
     1561 ggttgttgta cggcctcggg agggcgaggt tctcgggcac gccgagagtc gtgggcggcg
     1621 ggcacgccgc gttgcccggg cacgtgccga acgacggcgc gagcgacgtg aggaccaggc
     1681 atccgccggt tccccagtcg ctgaggatcg gctgctggta gtccacgccg ttcgcgcgcg
     1741 cgatgacggc gccgggcgag tcgacctgga cgcgcttcgt gaggcagttg gccgagtagc
     1801 cggcaccttc ggagtcgccg atccagacga gcctgctgag gtccacctgg gcggcggcgg
     1861 gaagcgcgag gacggccgcg acggcggccg cggagaggag gcggaggagg gtgtgaggag
     1921 tcttcatgtt cttcctggct ccctcagaag gtggtcttca tgccgacgct cgggaggatc
     1981 gcgaacgtct tgtatgtggc gctgaagttg tcgttgttcg tcgtgatggt gcgttcgtgg
     2041 aaccacagga agagcgacga gacgttgagc tggacgccgt ggaggacgtt gaagccggcg
     2101 ccgatcgtca cgccggtgcg gttgttgtcc ggcaggaacg gcgaggcgtc agcgtcgggc
     2161 tgcggcgtct ggtcgtagac gaacccggcg ccgagccagc ccttcttgcc cttgtagagc
     2221 gcgcccacgc ggacggccca ggtgttctcc cagtagtgcg ggaggaccga gtccggcagg
     2281 ccttcgatgc ggatgaccgt ctggtcgaac accttccagg tcacgtagtt gccgtccacc
     2341 gcgagcgtga ggttcggggt cacgtcgtac gcgacgccga aagccgtcag ggaggggaac
     2401 tgcagcgtcg tgcgccccgt ggggttcgtg ccgaacggca gcagcgtgga gaccgcggcg
     2461 tcgaactgcg ggctgcccgt ggcgatctgg gagaactggg cgggcccctc gaggtcgacg
     2521 tcgacgtgcg cgtggtacga gatgccgaag cgcaggcggt cgcacggctt gatgagcatg
     2581 ccggccgtcc acgtcacctt gatgggcgtg cccgtcgaga tgaggctcag gtgggcgatg
     2641 tcctggacgc gctgcgtgaa ggggttcacg gcctgcacgt tccgcgagag cttcacgtcg
     2701 gtgaaacgga cctcgggccc gccgccgatc gcgatcgcgt ccgagacctt ccacgcgaac
     2761 tgcgcgctca cggcgagctg gcggaggtcg gtgcgctggt tgatgacgcg gccgcggaac
     2821 gtgtccgggt tctgccacgg cacgccgaga ccgttcggcg accacatgcc cagcgacacc
     2881 ttgaagtcct tgctcagcga cgtcacgaag tacatgttcc cgaaccagta gagcggcttg
     2941 tgcatgttcg ccgagtagcc ttcgcccggg aacgggttca agccgtcgag ccgcgccgtg
     3001 ggccgcagga ctccgaagat gctgccgtcc acgatggtct cgtcgatgaa cgcgttgccg
     3061 gccgggttgt agaacatcgc cgacggatct tcggcggtgg ccgtgaacgc gcccgccatc
     3121 gcattgccct tcgaactctg ttcgaagagc gcgaagcccg cggcctcgag tcgggccgcc
     3181 ggaagggcgc ccgcgaggcc gacgagcacg agcgccgcca gccgggtccc aatcgctttg
     3241 aacaccggtt cctccttcac gtcgctatcc agaaagcgag caagtcgtct tttcggcccg
     3301 attctacaga caaaggcaag tctggcgacc ggcgtttcaa tccgtcaagg gtgcagccga
     3361 atcaaaccgc ttcccggcgc gtcaacgcgg gcgcgccccc agggtcgcga cggccacggc
     3421 gcccggtccg gcgtgggcgg ccaggacggg ggtgacctcc acgacgtact cctccgcccc
     3481 gggccgccgg gcgaggagtt cccgggcgat cctctcggcc agttcggcgg cgtcggcatg
     3541 ggccacggcg aaaaccggcg acgagacccc tgctgcggcc ttcagggact tctcgacgag
     3601 ccgtacgcag gcccggggga agccgcgggc ggcccctccg gcgcgggccc ggcccgccgc
     3661 gtcgagcgtc aggagcggaa ccacccccag caggttggcg aggcggcgct gtccggccgt
     3721 cacccggccc ccccggacga gcgagtccag ggacgggacg gcggcaaaaa gacgcacccg
     3781 gtccgcggcg tcctcggcgg tccggacgac ctcggca
//
LOCUS       DAOMFY010000334         3784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9331_length_3784_cov_6.550550, whole genome shotgun sequence.
ACCESSION   DAOMFY010000334 DAOMFY010000000
VERSION     DAOMFY010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9331_length_3784_cov_6.550550"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..569
                     /gene="rimI"
                     /locus_tag="PLB01_18040"
     CDS             99..569
                     /gene="rimI"
                     /locus_tag="PLB01_18040"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQQ79255.1"
                     /translation="MSALAESVTTRDAHAVDLDAIAALERAAFPVPWKREYFEVEIGA
                     AHRFNRVAIGPRGDLAGYVFCAFAAGELHVNKIATDERHRRRGIASHLMDEVLGFAEA
                     AGVEEVYLEVRVSNRPARAFYAGLGFREAGRRSRYYLDGEDALVLVRDMRKTAP"
     gene            642..878
                     /locus_tag="PLB01_18045"
     CDS             642..878
                     /locus_tag="PLB01_18045"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HQQ79256.1"
                     /translation="MQSLTEEIKHELASHNDEFKNLLKNHHDHERRLAELASKTFLST
                     DEEIEEKRLKKEKLHLKDRMEEIAREHRARVSAH"
     gene            888..1640
                     /gene="pssA"
                     /locus_tag="PLB01_18050"
     CDS             888..1640
                     /gene="pssA"
                     /locus_tag="PLB01_18050"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HQQ79257.1"
                     /translation="MVDSPSNAPRRGVRRGIYVLPALFTAGNIFCGFLSLDASAKGAF
                     ETAAILIFVAGFFDGIDGRVARLTGTTSAFGEQLDSLCDVVSFGVAPAFLVYNWGLSP
                     FGRAGLLVSFLFAVCGASRLARFNVQIHVVDKRWFVGLPIPSAAGALCGLIWAVPSTS
                     LTDPLRAVFLGVTAALAFLMVSTFRYRSFKDVDVKSRRGRLYFLLFAAVLALVWWLRQ
                     YALAGLLVTYAASAPLARFFGLFRRRPAGGTA"
     gene            1637..2623
                     /locus_tag="PLB01_18055"
     CDS             1637..2623
                     /locus_tag="PLB01_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79258.1"
                     /translation="MTKSAGVSVGLVEPTTLLGRDVKAVLKERRFPARKIHLFHQTPQ
                     PGGILTADDDEVAFVAPLTPDALETCTIAFLCGSAADTERFLASRTDSCLAIDLSGAR
                     REGALAVPSEGGATRALPSGNLLATPHPVAYVLAEAIAVVDALAPVAEATAAIDRPAS
                     ELGKAALDELFEQAVALAAFRPVPKAAFGTQAAFNTFVPADTVAFDARVAADLSALLG
                     RPLPLGILSARAGVFHGHFLRLELRLATDAPTSAALLAAFAARRDAFELVDPENLSGP
                     VESAGRDETLVLSARPEGRRVLLTLASDHLRRGGAILAVRLAEELLKERRIS"
     gene            complement(2620..>3784)
                     /locus_tag="PLB01_18060"
     CDS             complement(2620..>3784)
                     /locus_tag="PLB01_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79259.1"
                     /translation="EAGAEWARAVARVLADSGSWGRVAGVHVHIWPTPEQAKDLAAAL
                     AALKKSIGGAAVSVTLPAAGEPTAWKPLAGAADEALVFAFGRRPELGGRIVSEMPEDN
                     AKIFPIPFRLLVAFGSYGRAGDGTSFTGPVLADGKIDELSEDRGLDFEFGQQVFSADP
                     GTVYTFKPRPGARSSLTAAGGWARFQIPTMAEGLRSLSAAGRWAAPGYLGRVFLVGGV
                     PKDGSLIGYPAVRALLTGKPLEPRLAVEVAHAGSGHGWAEVTVTSTNLGPTPTDLSHY
                     NSYVQLRVEGGTVASVRAGDFDRYEQLTSEADGFKPTTSSRAVVCRLFENIFAPGEVD
                     VSGPIRVVGAHPHVFASWHLTLPDGKVVDGAEVEVQVPTAPVKTSPSKPARRR"
BASE COUNT          509 a         1432 c         1273 g          570 t
ORIGIN      
        1 atcgcggccg cggccgatcc ggcgcaggcc ggcggggtcg tgtacggccg gccgagcgcc
       61 gcggaggaga agctcgccgc cgcccgggaa ggggcgccat gagcgcgctg gccgagtccg
      121 tcacgacgcg cgacgcgcat gccgtcgacc tcgacgcgat cgccgcgctc gagcgcgcag
      181 cgtttcccgt gccctggaaa cgcgagtatt tcgaggtcga gatcggcgcc gcgcaccgct
      241 tcaaccgcgt cgcgatcggc ccgcggggcg acctcgcggg ctacgtcttc tgcgccttcg
      301 ccgcgggcga gctccacgtc aacaagatcg cgaccgacga gagacaccgg cgccgcggta
      361 tcgcctcgca cctgatggac gaggtgctcg gattcgccga ggcggccggc gtggaggagg
      421 tctacctcga ggtccgcgtc tcgaaccgtc cggcgcgggc gttctacgcc ggcctcggtt
      481 tccgggaagc cggccgcagg agccgctact acctggacgg ggaagacgcc ctcgttctgg
      541 tccgggacat gaggaaaacg gctccttgac ggcgcgcatg acgaaaatac agactcgcgg
      601 ccccggggct caccccggga tgccgatttg aggaggtggc gatgcagtcc ttgaccgagg
      661 aaatcaagca cgaactggcc agccacaacg acgagttcaa gaaccttctg aagaaccacc
      721 acgatcacga acgccggctg gccgagctcg cgtccaagac cttcctctcc acggacgagg
      781 agatcgagga aaagcggctg aaaaaggaaa agctccacct gaaggaccgc atggaagaga
      841 tcgcgcgcga acaccgggcg cgggtctccg cgcactaggc cttacccttg gtcgactccc
      901 cttcgaacgc cccgcgccgc ggggttcgcc gggggatcta cgtgctgccc gcgctcttca
      961 ccgcgggcaa catcttctgc ggctttctgt cactggacgc ctccgcgaag ggtgcgttcg
     1021 agaccgcggc gatcctcatc ttcgtcgcgg gcttcttcga cgggatcgac gggcgcgtcg
     1081 cccggctgac cggaacgacg tcggcgttcg gcgagcagct ggactccctg tgcgacgtgg
     1141 tctccttcgg cgtcgcgccc gcctttctcg tctacaactg ggggctctcc cccttcggcc
     1201 gcgcgggcct cctcgtctcg ttcctcttcg cggtctgcgg cgcctcgcgc ctcgcccgct
     1261 tcaacgtcca gatccacgtc gtggacaagc ggtggttcgt gggcctgccg atcccgtccg
     1321 cggcaggggc gctctgcgga ctcatctggg cggttccctc cacgtctctc acggacccgc
     1381 tccgcgccgt cttcctcggc gtgacggcgg cgctcgcgtt cctcatggtg tcgacgttcc
     1441 gctaccggtc cttcaaggac gtcgacgtca agtcgcgccg gggacggctc tacttcctgc
     1501 tcttcgcggc ggtgctcgcc ctcgtctggt ggctgcgcca gtacgcgctc gcgggcctcc
     1561 tcgtgacgta tgccgcgtcg gcgcctctcg cgcggttctt cggactgttc cgacggcgcc
     1621 ccgcgggcgg caccgcgtga cgaagtccgc gggtgtctcc gtcggcctcg tcgagccgac
     1681 cacgctcctc ggccgcgacg tgaaggccgt gctcaaggaa cgccggttcc ccgcccggaa
     1741 gatccacctg ttccaccaga ctccgcagcc gggcggcatc ctgacggcag acgacgacga
     1801 ggtcgccttc gtcgcgccgc tcactccgga tgcgctcgag acctgcacga tcgccttcct
     1861 gtgcgggtcg gccgccgaca cggaacgctt cctggccagc cgcacggact cgtgcctcgc
     1921 gatcgacctg tcgggcgcgc ggcgcgaagg cgctctcgcg gtgccctccg agggcggcgc
     1981 cacgcgcgcc ctcccgtcgg gaaacctcct cgcgacgccg caccccgtgg cttacgtcct
     2041 cgccgaggcg atcgccgtgg tggacgccct cgccccggtc gccgaggcca ccgcggcgat
     2101 cgaccggccc gcgagcgagc tcggcaaggc cgcactcgac gagcttttcg agcaggccgt
     2161 ggcgctcgcc gccttccggc cggtccccaa ggccgcgttc gggacccagg cggcgttcaa
     2221 cacgttcgtg cccgcggata cggtcgcctt cgacgcccgc gtcgccgccg acctctccgc
     2281 gctcctcggc cggcctctcc cgctcggcat cctttcggcg agggccggag tgttccatgg
     2341 ccatttcctg cgcctcgagc tgcgcctcgc caccgacgct cccacttccg ccgcgcttct
     2401 cgctgcgttc gcggcgcgca gggacgcctt cgagctcgtc gaccccgaga acctctcggg
     2461 ccccgtcgag tccgccgggc gcgacgagac gctcgtcctc tccgcgcgcc ccgagggccg
     2521 gcgcgtgctc ctcaccctgg cctcggacca tctgcggcgc ggcggcgcga tcctcgccgt
     2581 tcggctggcc gaagagctct tgaaagagcg gcggatctcc taacggcggc gcgcgggctt
     2641 cgacggcgac gtcttcaccg gcgccgtcgg cacctgcacc tcgacctccg cgccgtccac
     2701 gaccttgccg tccggcagcg tgaggtgcca gctcgcgaaa acgtgcgggt gcgcgcccac
     2761 gacgcggatc ggacccgaga cgtccacttc gcccggcgcg aagatgttct cgaagagccg
     2821 gcagaccacg gcgcggctgg acgtcgtcgg cttgaacccg tcggcctcgg acgtgagctg
     2881 ctcgtaccgg tcgaagtcgc ccgcgcggac ggacgcgacg gttccgccct ccacgcgcag
     2941 ctgaacgtag ctgttgtagt gcgagaggtc cgtcggcgtc ggacccaggt tcgtggacgt
     3001 cacggtgacc tcggcccagc cgtgtccgga ccccgcgtgc gcgacctcca cggcgaggcg
     3061 gggttcgagc ggcttgccgg tgaggagagc gcgcaccgcc ggatacccga tgagagatcc
     3121 gtccttcggg acgccgccga cgaggaacac gcggccgagg taccccggcg ccgcccaccg
     3181 gcccgcggcg gagagcgacc tcagtccctc ggccatcgtc ggaatctgga accgcgccca
     3241 gccgccggcc gccgtgaggg acgagcgtgc accgggccgc ggcttgaacg tgtacacggt
     3301 cccggggtcg gccgagaaca cctgctggcc gaactcgaag tcgagcccgc gatcctccga
     3361 cagctcgtcg atcttcccgt cggcgaggac cgggccggtg aagctcgtcc cgtcgccggc
     3421 ccggccgtag ctgccgaacg cgacgaggag gcggaacgga atcgggaaga tcttcgcgtt
     3481 gtcctcgggc atctcgctca cgatccgccc gccgagctcg ggcctccgcc cgaacgcgaa
     3541 gacgagcgcc tcgtcggcgg cgcccgcgag cggcttccag gccgtcggct cgccggcggc
     3601 cggcagcgtc acggacaccg ccgcccctcc gatggacttc ttcagcgccg cgagcgcggc
     3661 ggcgaggtcc tttgcctgct cgggagtcgg ccagatgtgc acgtgcacgc ccgccacgcg
     3721 gccccagctc ccggaatccg cgagcacgcg cgcgacggcc cgagcccact cggcccccgc
     3781 ctcg
//
LOCUS       DAOMFY010000335         3771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9394_length_3771_cov_6.812164, whole genome shotgun sequence.
ACCESSION   DAOMFY010000335 DAOMFY010000000
VERSION     DAOMFY010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9394_length_3771_cov_6.812164"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1573
                     /locus_tag="PLB01_18065"
     CDS             26..1573
                     /locus_tag="PLB01_18065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQQ79260.1"
                     /translation="MSAAPIRIVSPATGAFVGEVPDAGAAGAADAVARARAAQPEWEG
                     LDLEQRVEALKRWRDAVLDEPGVSETLVAESGKPRQEAEGIEVLYFCELIRAMTRLAP
                     GAFREETRHPLLFLTKKTLLVRRPLGVVAVIGPWNFPILNNAADAVAPLLAGNTVVLK
                     PSEVTPLTSILLRDLWLRAGNPPDVFQVVTGRGATGAALVDAADAVMFTGSVATGRRV
                     GARCGERLVPCVLELGGKSPFVVLAGANLERAAEAAAWSSFVHSGQACIRTERIFVEA
                     SVADRFERLLVARVSALRQMAPDSSGVIGHDLGSVTFARQIEVVEQHIADALSKGARV
                     LTGGARRDDLPGLFFQPTVLAGATPDMAVMREETFGPLVPVMRVSGADEALRLANDSH
                     LGLNATVFGPKAEAIAFARKLQSGQAIVNDVLVNYLVVESPLGGWKDSGLGARHGVEG
                     LRQWTRVEAITLRRPILAPVERFLARQLAFPYDPRVLAVLRRASRVLYRRGWVEKLKS
                     PRGRPRP"
     gene            1584..2705
                     /locus_tag="PLB01_18070"
     CDS             1584..2705
                     /locus_tag="PLB01_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79261.1"
                     /translation="MKRFLAALAALSFLSSAAAAQSGASAKKPTPLSAPGIQASTSTE
                     APKGANVSPGDMRAIQGEIQDPFELERLGLAAVNANDLDRARVFFEKAWKVGELPTAA
                     YNLACLDARKGRTDAAFTNLDKALAAGFDDGASLEKDPDLASLRGKPKWASVVSGAAK
                     NRAAGDAAAVKEGVFLMPKEKPLAILVLVHDKQSDPYSVSGPFADVALSHRLFVAAPR
                     GPGRSAKKRFGWGSADRAAKAIDAAIDAARRKAGNSKLPILIVGVGRGGTEVFTVAAK
                     KAPRTFEGVGSIGGPFDPGAAPSTAGLRGAKLFLGSSRDAEPNEINAARRGIEALRQQ
                     GFQPVVALWPGNAAGFPSDVPRAVKETLDAFAGVTPVTR"
     gene            2705..>3771
                     /locus_tag="PLB01_18075"
     CDS             2705..>3771
                     /locus_tag="PLB01_18075"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HQQ79262.1"
                     /translation="MAVDFYRHSLGDEEKDAVRAVLDSLFLTTGQKVYAFEKAFEEYL
                     GVPAVVCTAHATASLHIAMLAAGIGPGDEVVTTPMTFLSSANAVLYAGGTPVFADVDP
                     ATANIDPDAVEAAITPKTKAIVAVDLYGLLADMKALRKIADRYSLVLVEDAAHCVEAR
                     RDGVGPGQLADFACFSFYATKNLTCGEGGAISARDASKKNLLRQLGSHGMSRNAADRY
                     AGKYQHWDMERLGYKYNLSDVAASLLLPQLPKLAARLARREEICRRYEAAFQDVPGIT
                     FPVVPEGAMSARHLFTIWVEPADRDAILAGIQARGVGVAVNYRAVHLTSYYAERFGFT
                     RGMFPRAELIGDSTITIPLWPA"
BASE COUNT          509 a         1399 c         1327 g          536 t
ORIGIN      
        1 aggcggccgc gggtaggatg ctgccgtgag cgccgctccg attcgcatcg tctcgcccgc
       61 gacgggcgcc tttgtcggag aggttccgga cgccggggcc gccggcgccg ccgacgccgt
      121 cgcgcgcgcg cgcgccgcgc agcccgaatg ggaggggctc gatctcgagc agcgcgtcga
      181 agcgttgaag cgatggcgcg acgccgtgct cgacgagccg ggcgtgagcg agacgctcgt
      241 ggccgagagc ggcaagccgc gtcaggaggc cgaagggatc gaggtcctct acttctgcga
      301 gctgatccgc gccatgacgc gcctcgcgcc gggagcgttt cgggaggaga cccgccaccc
      361 cctcctcttc ctgacgaaga agacgctcct cgtccggcgc ccgctcggag tcgtcgccgt
      421 catcggcccg tggaatttcc cgatcctcaa caacgccgcc gacgcggtcg cgccgcttct
      481 cgccggaaac acggtcgtcc tcaagccgtc cgaggtcacg ccgctcacgt cgatcctcct
      541 gcgcgacctc tggctccgcg cgggcaatcc gccggacgtc ttccaggtcg tcacgggccg
      601 cggcgcgacg ggggctgccc tcgtggacgc cgcggacgcc gtgatgttca cgggctccgt
      661 cgcgaccggc cgccgcgtcg gggcgcgctg cggggagcgg ctcgtcccgt gcgttctcga
      721 gctcggaggc aagtcgccgt tcgtcgtcct cgcgggcgcg aacctcgagc gcgccgccga
      781 ggcggccgcg tggtcgagct tcgtgcactc gggccaggcg tgcatccgga cggagcgcat
      841 cttcgtcgag gcgtcggtcg ccgatcgctt cgagcggctt ctcgtcgcgc gcgtctcggc
      901 cctccgccag atggcgcccg actcctccgg agtcatcggg cacgacctcg gctccgtgac
      961 ctttgcgcgg cagatcgagg tcgtcgagca gcacatcgcg gacgccctgt cgaagggggc
     1021 gcgcgtcctg acgggcggcg cgcgccggga cgacctgccg gggctcttct tccagcccac
     1081 cgtcctcgcc ggcgcgacgc ccgacatggc cgtcatgcgc gaggagacgt tcggacccct
     1141 cgttcccgtc atgcgcgtgt cgggagcgga cgaggcgctc cgcctcgcga acgactcgca
     1201 cctcggcctg aacgcgaccg tcttcggccc gaaggccgag gcgatcgcct tcgcgcgcaa
     1261 gctccagtcc gggcaggcga tcgtcaacga cgtcctcgtg aactacctcg tcgtcgagtc
     1321 gccgctcggc ggctggaagg acagcggcct cggcgcgcga cacggcgtcg agggcctgag
     1381 gcagtggacg cgggtcgagg cgatcacgct ccgccgcccg atcctcgcgc ccgtcgagcg
     1441 cttcctcgcg cgccagctcg cgttcccgta cgaccccagg gttctcgcgg tcttgaggcg
     1501 cgcctcgcgc gtcctctacc gccgcgggtg ggtcgagaag ctgaagtccc cgcgcggccg
     1561 cccgcgcccg tagaatccgc cggatgaaac gcttcctcgc cgctctcgcg gccctgtcct
     1621 tcttgtcgtc cgccgcggcc gcccagtccg gcgcctcggc gaagaagccc acccctctct
     1681 cggcccccgg catccaggcg tcgacctcga cggaggcgcc gaagggcgcc aacgtgagcc
     1741 ccggcgacat gcgcgcgatt cagggtgaga tccaggaccc gttcgagctc gagcgcctcg
     1801 gcctcgcggc cgtcaacgcg aacgacctcg accgcgcgcg cgtgttcttc gagaaggcct
     1861 ggaaggtcgg cgagcttccg acggccgcct acaacctcgc ctgcctcgac gcgcgcaagg
     1921 gacggacgga cgccgcgttc acgaacctcg acaaggcgct cgcggccggc ttcgacgacg
     1981 gcgcgtcgct cgagaaggac ccggacctcg cctccctgcg cggcaagccg aagtgggcct
     2041 cggtcgtctc gggcgccgcg aagaaccgcg ccgcgggcga cgcggccgcc gtgaaggaag
     2101 gcgttttcct catgccgaag gagaagccgc tcgcgattct cgtgctcgtg cacgacaagc
     2161 agtcggaccc gtactccgtg agcggtccgt tcgcggacgt cgcgctctcg caccgcctct
     2221 tcgtggccgc gccgcgcggg ccgggccggt cggcgaagaa gcggttcggg tggggctcgg
     2281 cggaccgcgc cgcgaaggcg atcgacgccg ccatcgacgc cgcgcgacgg aaggcgggca
     2341 actcgaagct cccgatcctg atcgtcggcg tcgggagagg cggcaccgag gtgttcacgg
     2401 tcgccgcgaa gaaggcgccg aggacgttcg agggcgtcgg gtcgatcggg ggcccgttcg
     2461 accccggggc cgcgccgagc acggccgggc tgcgcggggc gaagctgttc ctcggctcct
     2521 cgcgcgacgc cgagccgaac gagatcaacg cggcgcgccg cgggatcgag gcgcttcgcc
     2581 agcagggctt ccagccggtc gtggccctgt ggccgggcaa cgcggcgggc ttcccgtcgg
     2641 acgtcccgcg cgccgtcaag gagacgctcg acgccttcgc cggggtgacg ccggtgacac
     2701 gctgatggcc gtcgacttct accgccactc gctcggcgac gaggagaagg acgcggtccg
     2761 cgccgttctc gactcgctgt tcctcacgac gggccagaag gtctacgcgt tcgagaaggc
     2821 gttcgaggaa tacctcggcg tgccggcggt cgtctgcacg gcgcacgcca cggcctcgct
     2881 ccacatcgcg atgctcgcgg ccggcatcgg gcccggagac gaggtcgtca cgacacccat
     2941 gacgtttctc tcgtcggcga acgccgtgct ctacgcgggc gggacgcccg tgttcgcgga
     3001 cgtggacccg gcgaccgcga acatcgaccc ggatgccgtc gaggccgcga tcaccccgaa
     3061 gacgaaggcg atcgtcgcgg tggacctcta cgggctcctc gcggacatga aggcgctgcg
     3121 gaagatcgcg gaccgctact ccctcgtcct cgtcgaggac gccgcgcact gcgtcgaggc
     3181 ccggcgcgac ggggtcggcc cggggcagct cgccgacttc gcgtgcttca gcttctacgc
     3241 gacgaagaac ctgacgtgcg gcgagggcgg cgcgatctcc gcccgcgacg cgtcgaagaa
     3301 gaacctgctc cgccagctcg gctcgcacgg gatgtcccgc aacgccgccg accgctacgc
     3361 ggggaagtac cagcactggg acatggagcg cctcggctac aagtacaacc tctccgacgt
     3421 cgcggcgtcg ctccttctcc cgcagctccc gaagctcgcg gcccgcctcg cgcggcgcga
     3481 ggagatctgc cgccgctacg aggccgcctt ccaggacgtg cctgggatca cgttcccggt
     3541 cgtgcccgag ggcgcgatgt cggcgcgcca cctcttcacg atctgggtcg agccggccga
     3601 ccgcgacgcg atcctcgcgg ggatccaggc gcgcggcgtc ggcgtcgccg tcaactaccg
     3661 cgccgtccac ctgacgtcgt actacgccga gcgtttcggg ttcacgcgcg gaatgttccc
     3721 gagggccgag ctcatcggcg actcgacgat cacgatcccg ctctggcccg c
//
LOCUS       DAOMFY010000336         3735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9558_length_3735_cov_6.675543, whole genome shotgun sequence.
ACCESSION   DAOMFY010000336 DAOMFY010000000
VERSION     DAOMFY010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_3735_cov_6.675543"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..950)
                     /locus_tag="PLB01_18080"
     CDS             complement(42..950)
                     /locus_tag="PLB01_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HQQ79263.1"
                     /translation="MNDSRLREAIGRVVAGAENRKLLSAIDAPPKPRSRELVEILRML
                     REVVYPGFYGEPGVSRATLGDRLEEVLRRIHATLSSEIEKALLPDDPSGAAARAPELA
                     TGLIEALPAIADMVADDVRAAFDGDPAATGFDEIILAYPGVMAIFTYRVAHALHVAGV
                     PLIPRMMTEFAHLETGVDIHPGARIGRSFFIDHGTGIVIGETTDIGKNVKLYQGVTLG
                     ALSFPKNERGELIRGTKRHPTVEDDVVIYAGATILGGETVVGRGSVIGGSVWLTASVP
                     PGTRVTLQGDQLKREPRVAAAAPARR"
     gene            complement(964..1833)
                     /locus_tag="PLB01_18085"
     CDS             complement(964..1833)
                     /locus_tag="PLB01_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79264.1"
                     /translation="MRAALLVLAIPLAFFGPAFADDEAPLAEADRLWSLRADGSTGGR
                     ARAEAIDPVIAACRTALEKEPDSVAARGRLMRALYFKGEHVADDVTTKRDIFDEGRKV
                     AEQALGLLRRDASKVSGRDLSKGTPVDLAPHLRGRADAVDAFYWAAADWGKWSLAFGK
                     MAAVKQGAAAKIRDYAQAVILLDPSFNGGGGYRILGRLHHQTPSVPFLTGWASRAEAL
                     KNLRLAVATGPKDFLGRQFLAEAIWDYEPDKRAEARALMQVLVNDTPQPEWLVECRRS
                     QEDAAAKLREWGK"
     gene            1898..2224
                     /locus_tag="PLB01_18090"
     CDS             1898..2224
                     /locus_tag="PLB01_18090"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HQQ79265.1"
                     /translation="MADEPKTEGPQPPTLQFSIDDTVANGVYVNFANIIHNPAEFVLD
                     FGRVVPGRTDVRVLSRVLTSPVHAKQLLNALGQNVALYEKTFGPIRTDFEAAAAPPST
                     DPTRPN"
     gene            2258..2779
                     /locus_tag="PLB01_18095"
     CDS             2258..2779
                     /locus_tag="PLB01_18095"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQQ79266.1"
                     /translation="MPLSRRFESAVLALLVFASAAPAFAAEPAQKKQTPASAPAAPAG
                     PIYDPDLLGEQAIAAAQKVAADSGRRLLVNLGTNDCAPCVVFNRALHKDKFFGAFIEQ
                     FIPAWIDVSNLPNAALIDKYAINPKAELPAILIFMPDGRAIEALAHGEMAAIAKNGEA
                     AVQEWLLKRFAKQ"
     gene            2805..3404
                     /locus_tag="PLB01_18100"
     CDS             2805..3404
                     /locus_tag="PLB01_18100"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQQ79267.1"
                     /translation="MAVSAHSAAAYGPDPGDLLLVAAGDDAALRRLLSRWKQPVYAAF
                     ERLREPSAAAEATLLTFERLVRSAGRYEPGASFPATLWEHAARVAQELPAVEPVSVSP
                     ARLAESVAARTALQRSALAALPPAERAAFLLTRVARLPLATAAAALGTSESEVRRRLV
                     RALETLRRSLRPLLDLGAGGADPSSDASATRPPPIGDAP"
     gene            3401..>3735
                     /locus_tag="PLB01_18105"
     CDS             3401..>3735
                     /locus_tag="PLB01_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79268.1"
                     /translation="MTCDELRERIVDPASAGQGGHAPVVEHLEQCAACRASARDFAAI
                     DRLLKPAAAPAPPADLDVRLLQRLSAPRGNPWMQPAVLLAAAAAVLVLAFFGARALRR
                     APAVDNAAT"
BASE COUNT          517 a         1432 c         1233 g          553 t
ORIGIN      
        1 gcagcccgcg gggccggcgc cgacgacgac cgcgtcccag gtcatcgccg ggcgggtgcg
       61 gcggccgcga cgcgcggctc gcgcttcagc tggtcgccct ggagcgtgac gcgcgtcccg
      121 ggcggaaccg acgcggtgag ccacacgctg ccgccgatca cggacccgcg cccgaccacg
      181 gtctcgccgc cgaggatcgt cgcgcccgcg taaatcacga cgtcgtcctc gaccgtcggg
      241 tggcgcttcg tcccgcggat gagctcgccg cgctcgttct tcgggaacga gagcgcgccg
      301 agcgtgacgc cctggtacag cttgacgttc ttcccgatgt ccgtcgtctc gccgatgacg
      361 atcccggtgc cgtggtcgat gaagaacgag cgcccgattc gcgcgccggg gtggatgtcg
      421 acgcccgttt cgaggtgggc gaactccgtc atcatgcgcg ggatgagcgg cacgcccgcc
      481 acgtgcaggg catgggcgac gcggtacgtg aagatggcca tgaccccggg ataggcgagg
      541 atgatctcgt cgaaacccgt cgcggcggga tcgccgtcga acgcggcgcg cacgtcgtcg
      601 gcgaccatgt ccgcgatcgc gggcagggcc tcgatgagcc ccgtcgcgag ctccggagcg
      661 cgcgcggcgg cgcccgaggg gtcgtccggc aggagcgcct tctcgatctc gctcgagagc
      721 gttgcgtgga tccggcgcag gacctcctcg aggcggtcac ccagggtcgc ccgcgagacc
      781 ccgggctcgc cgtagaaacc cgggtacacg acctcccgga gcatccggag gatctcgacg
      841 agctcgcggc tgcgcggctt cggaggcgcg tcgattgcgg agagcagttt ccggttttcg
      901 gcgcctgcga ccacgcggcc gatcgcctcg cggaggcggg agtcgttcat gcgactcatt
      961 ctctcacttg ccccactccc ggagcttcgc cgccgcgtcc tcctgcgacc gccggcattc
     1021 gacgagccac tccggctgcg gggtgtcgtt cacgaggacc tgcatcagcg cccgcgcctc
     1081 ggcgcgcttg tcgggctcgt agtcccagat cgcctcggcg aggaactggc gcccgaggaa
     1141 gtccttcggt ccggttgcga ccgcgagcct caggttcttc aacgcctccg cgcgggatgc
     1201 ccagcccgtc aggaacggga cggacggcgt ctggtggtgg agacggccga ggatccggta
     1261 gccgccgccg ccgttgaacg aggggtcgag gaggatgacc gcctgcgcat agtcccggat
     1321 cttcgccgcc gcgccctgct tgacggcagc catcttcccg aacgcgagcg accacttgcc
     1381 ccagtcggcg gccgcccagt agaaggcgtc gaccgcgtcg gcgcggcccc tgagatgagg
     1441 cgcgagatcg acaggcgtcc ccttcgagag gtcccggccc gaaaccttcg acgcgtcgcg
     1501 acgcagcagt ccgagcgcct gctcggcgac cttgcggcct tcgtcgaaga tgtccctctt
     1561 cgtcgtgacg tcgtccgcga cgtgctcgcc cttgaagtac agcgcgcgca tcagccggcc
     1621 gcgcgcggcg acggagtcgg gctccttctc gagcgcggtt cggcacgcgg cgatcacggg
     1681 gtcgatggcc tcggctcggg cacgcccgcc cgtcgatccg tccgcacgga gcgaccagag
     1741 gcggtcggcc tcggcgagtg gcgcctcgtc gtcggcgaaa gcaggcccga agaaagcgag
     1801 agggatcgcg aggaccagaa gggcggctct catccgtgac actatactgg cgcgcctttt
     1861 gcgaggaggc tcccgattcg acctgcgagg tgccgccgtg gccgacgaac cgaagaccga
     1921 aggaccccag cctcccaccc tgcaattctc gatcgacgac accgtcgcga acggcgtcta
     1981 cgtgaacttc gcgaacatca tccacaaccc ggccgagttc gtcctcgact tcggccgcgt
     2041 cgttcccggg cgcacggacg tccgcgttct ctcgcgcgtg ctgacctccc ccgtccacgc
     2101 gaaacagctc ctgaacgccc tcggccagaa cgtcgcgctc tacgagaaga cgttcggccc
     2161 gatcagaacc gacttcgaag cggccgccgc gccgccctcg accgacccga ctcggccgaa
     2221 ctgaagacca aaggccaccc aaggagactt gaccccgatg cctctctccc gtcgcttcga
     2281 atccgccgtc ctcgccctcc tcgtcttcgc cagcgccgcg ccggccttcg cggccgagcc
     2341 cgcgcagaag aagcagacgc cggcgtccgc gcccgccgcg cccgccggac cgatctacga
     2401 cccggatctc ctcggtgagc aggcgatcgc ggccgcgcag aaggtcgccg ccgactcggg
     2461 ccgccgcctc ctcgtcaacc tcggcacgaa cgactgcgcc ccgtgcgtcg tcttcaaccg
     2521 cgcgctccac aaggacaagt tcttcggcgc gttcatcgaa cagttcatcc ccgcgtggat
     2581 cgacgtctcg aacctgccga acgccgctct gatcgacaag tacgccatca acccgaaggc
     2641 ggagctcccc gcgatcctca tcttcatgcc cgacgggcgc gccatcgagg cgcttgcgca
     2701 cggcgagatg gccgcgatcg cgaagaacgg tgaagccgcc gtccaggagt ggctcctcaa
     2761 gcgcttcgcg aaacagtagc cggagcttct ggcagaatga ccctgtggcc gtctccgcgc
     2821 actccgcggc cgcgtacggg ccggaccccg gcgacctcct tctcgtggcg gccggggacg
     2881 acgcagcgct gaggcgtctc ctttcgcgct ggaaacaacc cgtctacgcc gccttcgaac
     2941 gcctccgcga gccgtccgcc gctgcggagg cgacgctcct gacgttcgaa cgcctcgtgc
     3001 gctcggccgg ccgttacgag cccggtgcgt cgttcccggc gactctctgg gagcatgccg
     3061 cgcgcgtcgc gcaggagctt cccgcggttg aacccgtctc cgtctcgccc gcgcgcctcg
     3121 cggaatccgt tgccgcgcgc acggcgctgc agcgctccgc gctcgcagct cttccgccgg
     3181 ccgagcgcgc cgccttcctt ctcacccgtg tcgcgcgcct cccgctggcc acggccgccg
     3241 cggccctcgg aacgtccgaa tccgaagtcc gccggcgcct cgtgcgtgcg ctcgagacgc
     3301 tccgcagatc ccttcgtccc ctgctcgacc tcggcgctgg cggcgccgat ccgtcgtccg
     3361 acgcttccgc gacgaggccg ccgccgattg gagacgcgcc gtgacctgcg acgagctgcg
     3421 cgagcgcatc gtcgatcccg cgtccgccgg gcagggcggc cacgcacccg tcgtcgagca
     3481 cctcgagcag tgcgcggcct gccgcgcctc cgcccgcgac ttcgcggcga tcgaccgtct
     3541 cctgaaaccc gccgcggcgc ccgcgccgcc ggccgacctc gacgtccggc ttctccagcg
     3601 gctctccgcg cctcgcggga atccgtggat gcagcccgca gtgctcctcg ccgccgccgc
     3661 cgcggttctc gtcctggcct tcttcggggc ccgcgcgctg cgccgcgcgc cggccgtcga
     3721 caacgccgcg actga
//
LOCUS       DAOMFY010000337         3732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9571_length_3732_cov_9.130813, whole genome shotgun sequence.
ACCESSION   DAOMFY010000337 DAOMFY010000000
VERSION     DAOMFY010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9571_length_3732_cov_9.130813"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PLB01_18110"
     CDS             <1..603
                     /locus_tag="PLB01_18110"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQQ79269.1"
                     /translation="APDSFIPLAEDTRLIVPMGEWILHEACAQASKFPSRAGSSWFVA
                     VNLSARQFHRGGLVKTVEDALRASGLAPDRLELEITESVAMGDIQEAISTLNRLRGMG
                     VRVVLDDFGTGHSSLAYLKSLPLDAIKIDRTFVAAVPGTGADASLVRAILEMARGLDL
                     RVIAEGVETEEQLAFLMAHGCMQAQGFLFGAPVPVARFGL"
     gene            complement(604..3687)
                     /locus_tag="PLB01_18115"
     CDS             complement(604..3687)
                     /locus_tag="PLB01_18115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ79270.1"
                     /translation="MKASALLCAGLVAAAAAVAADQAPPASGTRPTPPPLVSVAPPVP
                     EAQMARSPEALKLFGRLDDDRAVVFIASGPADARVTVRCPDLFRPVEIWTYLEHPTLG
                     KNARILFYPETPTGSYRYWTVLEGEAALFPPSDKTVSLADVEKNAGACADLAVLVSAV
                     KDIARRQGDNNGGLAERGALAVPHFVPPPGAKVEAPAPLLVSNKPLTTKERKKLTAEA
                     PERVRRFLDDVEPIITDVERDTLLKLTEDYQRDRFVDDFWKRRSTAPDGMRTPFRDIY
                     EMRLAEAKRLFGNINTDMGRIFLLHGPPDGMRKIDCQDIYWPLQIWYYERIEELRMSK
                     VLLLFYQQLGVGDYRLWTPLDGQGALIVGNIAGGLSGGPGGRRVDVTRCSEYRDVMAA
                     VNTVAATFGTIGGMKMADDLKTAAKPDVEGADRILQLTTDIPAGAAVLPIQRVFRFPE
                     LVGSRMRMELAVLLERESLTKKQIGEESFYDIDLVGEVVRDGRLVDNFRYRFDFPVSS
                     VNGPFVPLTIERELYPGEYQFRVKVQDANRNAAALIKERLKVPDAPEAAMTAEEKAAR
                     EAAKRAVTELVENAGAPRGSLSFLPIAREIATGLIRFEARASSNDIAFTEFFLNNTRV
                     VTKRRPPFAADLDLGELPRKHVVKVIGYSKDGQAIAQDEMILNEGREAFRIRITSPAK
                     GAALVGAVRVVADLAVPETKRLEKVEFYVNDRRAATLYQEPFQQVVDVPKSADLGFLR
                     VVATLEDGQSTEDLRYYNAPKYLSEENVKAVELYTSVLAKGRPVTGLKKEAFQILEDG
                     VPQDLDGFEIVTNLPLSLGIGVDTSGSMEESIVEAQKAAVEFLKSVMTKKDRSFLVTF
                     DNEPQLVSRFTTDRDKLAQALAGLRAQGSTALWDALVYGLYQYQGTKGRKAYVILTDG
                     EDRSSHFTFDAALDYAKKTGVAVYFIGLRIGSTQIDVRHKLGKIARETGGTVYYVDNA
                     KGLARIYAEINEELRSQYLLSYVPQNKSLTTQWRRIEVKMTPSNLTARTISGYYP"
BASE COUNT          532 a         1258 c         1301 g          641 t
ORIGIN      
        1 gcgcccgact ccttcatccc gctcgcggag gacacgcgcc tgatcgtccc gatgggcgag
       61 tggatcctgc acgaagcctg tgcgcaggcg tcgaaattcc cgtcaagggc cgggagctcg
      121 tggttcgtgg ccgtcaacct ctcggcgcgg cagttccacc gcggcggcct cgtcaagacc
      181 gtcgaggacg ctctccgggc atcgggcctg gcgccggacc ggctcgagct cgagatcacc
      241 gagagcgtcg cgatgggcga catccaggag gcgatctcga cgctgaaccg gctgcgcgga
      301 atgggcgtcc gcgtcgtcct cgacgacttc ggcacgggcc actcctcgct cgcgtacctc
      361 aagagcctgc cgctcgacgc gatcaagatc gaccgcacgt tcgtggccgc ggtgcccggg
      421 accggcgcgg acgcttcgct cgtccgggcg atcctcgaga tggcgcgggg cctcgacctg
      481 cgcgtcatcg ccgagggcgt cgagacggag gagcagctcg ccttcctgat ggcgcacgga
      541 tgcatgcagg cgcaggggtt cctcttcggc gcgcccgttc ccgtcgcgcg cttcggcctc
      601 tgactagggg tagtacccgc tgatcgtccg cgccgtcagg ttcgaagggg tcatcttgac
      661 ctcgatccgc cgccactgag tcgtcaggga cttgttctgc gggacgtacg agagcaggta
      721 ctggctccgg agctcctcgt tgatctccgc gtagatccgc gcgagcccct tcgcgttgtc
      781 gacgtagtac acggtgccgc cggtctcgcg cgcgatcttg ccgagcttgt gccgcacgtc
      841 gatctgcgtc gagccgattc ggaggccgat gaagtagacc gcgacgcccg tcttcttcgc
      901 gtagtcgagc gccgcgtcga aggtgaagtg cgaggagcgg tcctcgccgt ccgtcaggat
      961 gacgtacgcc ttccgcccct tcgttccctg gtactggtag aggccgtaca cgagcgcgtc
     1021 ccacagcgcc gtcgagccct gcgcgcggag acccgcgagc gcctgcgcga gcttgtcgcg
     1081 gtcggtcgtg aagcgcgaga cgagctgcgg ctcgttgtcg aacgtgacga ggaacgagcg
     1141 gtccttcttc gtcatgacgc tcttgaggaa ctccacggcc gccttctggg cctcgacgat
     1201 cgactcctcc atcgaccccg acgtgtcgac gccgatcccg agcgagagcg gaaggttcgt
     1261 gacgatctcg aatccgtcga ggtcctgcgg aacgccgtcc tccaggatct ggaacgcctc
     1321 cttcttgagg cccgtcacgg gacggccctt cgcgagaacc gacgtataga gctcgacggc
     1381 cttgacgttc tcctcggaga ggtatttcgg cgcgttgtag tagcggaggt cctcggtgga
     1441 ctggccgtcc tcgagggtcg cgacgacgcg caggaagccg aggtccgcgc tcttcgggac
     1501 gtcgacgacc tgctggaacg gttcctggta gagcgtcgcg gcgcgccggt cgttcacgta
     1561 gaactcgacc ttctcgagcc gcttcgtctc gggcaccgcg agatccgcga cgacacgcac
     1621 ggcgcccacg agcgccgcgc ccttggccgg gctcgtgatc cggatgcgga acgcctcgcg
     1681 gccctcgttc aggatcatct cgtcctgcgc gatcgcctgc ccgtccttcg agtagccgat
     1741 caccttgacc acgtgcttgc gcggcagctc cccgaggtcg aggtcggcgg cgaagggcgg
     1801 gcggcgcttc gtgaccacgc gcgtgttgtt caggaagaat tcggtgaacg cgatgtcgtt
     1861 cgagctcgcc cgcgcctcga accggatgag gcccgttgcg atctcgcggg cgatcgggag
     1921 aaacgacagg ctgccccggg gcgcgccggc gttttccacg agctcggtga cggcccgctt
     1981 ggcggcctcg cgcgccgcct tttcctcggc cgtcatcgcg gcctccggcg cgtcggggac
     2041 cttcagccgc tccttgatga gtgccgccgc gttgcggttc gcgtcctgga ccttcacgcg
     2101 gaactggtac tcgcccgggt agagctcgcg ctcgatcgtg agcggcacga acggcccgtt
     2161 cacgctcgag accgggaagt cgaagcgata ccggaaattg tcgacgagcc gaccgtcccg
     2221 gacgacctct ccgacgaggt cgatgtcgta gaagctctcc tcgccgatct gtttcttcgt
     2281 cagcgactcg cgctcgagga ggacggcgag ctccatccgc atgcggcttc cgacgagctc
     2341 ggggaagcgg aagacgcgct ggatgggcag caccgccgcg ccggccggga tgtccgtcgt
     2401 gagctggagg atccggtctg cgccctcgac gtcgggcttg gcggccgtct tgaggtcgtc
     2461 ggccatcttc atgccgccga tcgttccgaa ggtcgccgcg acggtgttca cggcggccat
     2521 gacgtcgcgg tactcgctgc agcgcgtcac gtcgacgcgg cgccccccgg ggcccccgct
     2581 caggccgccc gcgatgttcc cgacgatgag cgcaccctgc ccgtccagcg gagtccagag
     2641 gcggtagtcc ccgaccccga gctgctggta gaagaggagg agcaccttgg acatccggag
     2701 ctcctcgatt cgctcgtagt accagatctg gagcggccag tagatgtcct ggcagtcgat
     2761 cttgcgcatg ccgtccggcg ggccgtgcag gaggaagatc cggcccatgt ccgtgttgat
     2821 gtttccgaag agacgcttgg cctcggcgag gcgcatctcg tagatgtcgc ggaacggggt
     2881 ccgcatcccg tcgggggcgg tcgagcggcg tttccagaag tcgtccacga accggtcgcg
     2941 ctggtagtcc tcggtcagct tgaggagcgt gtcgcgctcg acgtccgtga tgatcggctc
     3001 gacgtcgtcg aggaagcgcc ggaccctttc gggcgcctcc gcggtgagct tcttgcgttc
     3061 cttggtggtc agcggcttgt tcgagacgag gagcggcgcg ggcgcctcga ccttggcgcc
     3121 gggtggcggg acgaagtgcg gcaccgcgag cgcgccgcgc tcggcgagcc cgccgttgtt
     3181 gtcgccctgg cgccgcgcga tgtccttcac ggccgacacg agcacggcga ggtccgcaca
     3241 ggcgccggcg ttcttttcga cgtccgcgag cgacaccgtc ttgtcggacg gcggaaagag
     3301 agccgcctcg ccctcgagga ccgtccagta gcggtacgat ccggtcggcg tctccggata
     3361 gaagaggata cgcgcgttct ttccgagcgt cgggtgttcg aggtacgtcc agatctcgac
     3421 gggacggaac aggtccggac accgcacggt gacgcgggcg tccgccggcc cggaggcgat
     3481 gaagacgacg gcacggtcgt cgtccaggcg gccgaagagc ttcagggcct ccggagagcg
     3541 ggccatctgg gcctcgggaa cgggcggtgc gaccgagacg agcgggggcg gcgtggggcg
     3601 cgtacccgaa gccggcggcg cctggtcggc ggccacggcg gccgcggccg ccacgagccc
     3661 cgcgcagaga agtgccgacg ccttcatgcc ggtcattgtc cagctttcca tccgcgtacg
     3721 cacaccttcc cg
//
LOCUS       DAOMFY010000338         3722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9609_length_3722_cov_8.168257, whole genome shotgun sequence.
ACCESSION   DAOMFY010000338 DAOMFY010000000
VERSION     DAOMFY010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9609_length_3722_cov_8.168257"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..291
                     /locus_tag="PLB01_18120"
     CDS             40..291
                     /locus_tag="PLB01_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79271.1"
                     /translation="MNATAIGLTVGIALLGLLVILIGIFPSPEDPAGRPPGRKQIEKV
                     LLRLRRDPVFHESFAGRRLSATPRVVRLTPSKKKSSETS"
     gene            358..924
                     /locus_tag="PLB01_18125"
     CDS             358..924
                     /locus_tag="PLB01_18125"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQQ79272.1"
                     /translation="MAIAFDERYGPADIARRVADVGRRIAHDYPNGNIVLIAILKGAG
                     FFVADLARAVPRPVRCEYIDVLRDGEAEKVVNYHFLAPFDVRDAHLIVLKDVIRSGIV
                     ETWLLSQLREENPRSLRFACVVDRPQERKSSLLADYVLFPSEEGVLVGYGMEYQREGG
                     NLPFIAQVRTEGESPFETSGEIARRTRP"
     gene            974..2896
                     /gene="ftsH"
                     /locus_tag="PLB01_18130"
     CDS             974..2896
                     /gene="ftsH"
                     /locus_tag="PLB01_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQQ79273.1"
                     /translation="MNTTVKNILLWMVILVVILLLFRVVDVGRTNTATVSFTEFMEMV
                     KKGDVEKVTIRGQEIRGFKKGAVEGREPGVRAYAPAYNDLTSDLMKANVKITAEEPRE
                     GSFLVILLQWAPILFLIGLWIFIMRQMQTGGNRAMQFGKSRAKMLTPNQKKVTFKDVA
                     GCEEAKEELSEIIDFLKEPAKFQKLGGKIPKGVIMMGPPGTGKTLLARAVAGEANVPF
                     FSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNALLVEMDGFEGNDGVILVAATNRPDVLDPALLRPGRFDRRVVVDRPDVKGREG
                     ILKVHSRTIPMSDDVDLSVVARGTPGFAGADLANLVNEAALAAARVDKKKVDMSDFNY
                     AKDKVLMGAERKSMVMSDEEKRVTAYHEGGHALIASFEEHTDPLHKVTIIPRGRALGL
                     TQQLPLEDKYTYSKEYIEAELAVMMGGRLAEEVCIGRITTGASNDFEKATEMARRMVC
                     EWGMSELGPMSFGRPEGEVFLGRDFSRQPDYSEDTARKIDDEVNRIVTTSYARGKKII
                     VEHRAALEEIAKMLLEKESLDGEEIYAIIKRVTGHEVAKRPPVKPEPPKPTAPATVAQ
                     KDEGAAPAGGLIPVPA"
     gene            2898..>3722
                     /gene="folP"
                     /locus_tag="PLB01_18135"
     CDS             2898..>3722
                     /gene="folP"
                     /locus_tag="PLB01_18135"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQQ79274.1"
                     /translation="MDFHLTLPGGRELALGALPGVMAILNVTPDSFSDGGRFASAAAA
                     ADEGARLADLGVTLVDVGGESTRPKGATYGAGATEIFLEGELGRVLPVISRLRGLRPS
                     LPISVDTRRVEVARAALDEGADVVNVVTGLNVPKDLLDLVASRGVPIVLNHCRGTPST
                     TFEESSFTDVIAEVRADLNGARSRALSSGVRAESVFLDPGLGFGKTPEQNFALLGALD
                     RLAPPGAPLVVGASRKAFLGTVAGSPVSDRLPESLAAAAVCAEAARTRPVLVRVHDA"
BASE COUNT          622 a         1289 c         1255 g          556 t
ORIGIN      
        1 gctgcagcgc gcagacgcta tgcaactggg tacggcagca tgaacgcgac agcgatcggc
       61 ctgacagtcg ggatcgcgct gctgggcctg ctcgtgattc tgatcgggat cttcccctcg
      121 ccggaagacc ccgccggccg gccccccggc cgcaaacaga tcgagaaggt gctgctccgc
      181 ctgcgccgcg atcccgtgtt ccacgagtcg tttgcgggtc gaagactgag cgccaccccg
      241 cgggtcgtcc ggcttacacc ttctaagaag aaatccagcg agacgtcctg aacggcccca
      301 actcgacgcg cgcgggtccg aatcgggtcc ccgggccccc cttgatagac ttcccccgtg
      361 gccatcgcct tcgacgaacg gtacggtccc gccgacatcg cgcggcgtgt ggcggacgtc
      421 gggcggcgga tcgcccacga ttacccgaac ggcaacatcg tcctcatcgc gatcctcaag
      481 ggtgccgggt tcttcgtggc ggacctcgcc cgggcggtgc cgcgccccgt gcgctgcgag
      541 tacatcgacg tgctccgcga cggggaggcc gagaaggtgg tcaactacca cttcctcgcg
      601 cccttcgacg tgcgggacgc ccacctgatc gtcctcaagg acgtcatccg cagcgggatc
      661 gtggagacct ggctgctcag ccagctccgg gaagaaaacc cccgttccct gcgtttcgca
      721 tgtgtggtgg atcgaccgca ggaacgcaag agctcgcttc tcgccgatta cgtgctgttc
      781 ccgtccgagg aaggggtcct cgtcggatac ggcatggaat accagcggga agggggaaat
      841 ctccccttca tcgcccaggt ccggaccgaa ggggaatcgc cgttcgagac gagcggcgag
      901 atcgcccggc gcacgcggcc ctgaaccgcg tgggcccgtc tgacgttcag taaggaagcc
      961 gaggcatttc aaggtgaata caaccgtcaa gaacatcctg ctgtggatgg tcatcctcgt
     1021 ggtgatcctc ctcctgttcc gcgtcgtcga cgtcggccgc acgaacacgg ccacggtctc
     1081 gttcaccgag ttcatggaga tggtgaagaa gggcgacgtc gagaaggtca cgatccgcgg
     1141 gcaggagatc cgcgggttca agaagggcgc cgtggagggc cgcgagcccg gcgtgcgcgc
     1201 gtacgccccg gcctacaacg acctcacgtc cgacctcatg aaggcgaacg tcaagatcac
     1261 ggcggaggag ccgcgcgagg gctcgttcct cgtgatcctc ctgcagtggg ccccgatcct
     1321 gttcctgatc ggcctgtgga tcttcatcat gcggcagatg cagacgggcg ggaaccgcgc
     1381 gatgcagttc gggaagtcgc gcgccaagat gctcacgccg aaccagaaga aggtcacgtt
     1441 caaggacgtc gcgggctgcg aggaagccaa ggaagagctc tccgagatca tcgacttcct
     1501 gaaggagccc gcgaagttcc agaagctggg cggcaagatc ccgaagggcg tcatcatgat
     1561 gggccccccc ggcacgggaa agacgctcct cgcgcgcgcg gtcgcgggcg aggcgaacgt
     1621 gccgttcttc tcgatctcgg gctcggactt cgtcgagatg ttcgtgggcg taggcgcgag
     1681 ccgcgtccgt gacctcttcg agcagggcaa gaagaacgcg ccctgcatca tcttcatcga
     1741 cgagatcgac gcggtcggcc gccaccgcgg cgccggcctc ggcggcgggc acgacgagcg
     1801 cgagcagacg ctcaacgcgc tcctcgtcga gatggacggc ttcgagggca acgacggcgt
     1861 catcctcgtc gcggccacga accggcccga cgtcctcgac cccgcgctcc tccggcccgg
     1921 ccgcttcgac cgccgcgtcg tcgtggaccg ccccgacgtg aagggccgcg agggcatcct
     1981 gaaggtccac tcgcgcacga tcccgatgtc cgacgacgtc gacctctcgg tcgtggcccg
     2041 ggggacgccc ggcttcgccg gagccgacct cgcgaacctc gtgaacgagg cggcgctcgc
     2101 cgccgcgcgc gtcgacaaga agaaggtcga catgtccgac ttcaactacg ccaaggacaa
     2161 ggtcctcatg ggggcggagc ggaagtcgat ggtcatgtcg gacgaggaga agcgggtcac
     2221 cgcgtaccac gagggcggcc acgcgctcat cgcctcgttc gaggagcaca cggacccgct
     2281 ccacaaggtc acgatcatcc cgcgcggccg cgctctgggc ctcacccagc agctgccgct
     2341 cgaggacaag tacacgtact cgaaggagta catcgaggcc gagctcgccg tcatgatggg
     2401 cgggcgcctc gccgaggaag tctgcatcgg ccggatcacg acgggcgcct cgaacgactt
     2461 cgagaaggcc accgagatgg cgcgccgcat ggtttgcgag tggggcatga gcgagctggg
     2521 gccgatgtcg ttcggccggc ccgagggcga ggtgttcctc ggccgcgact tctcgcgcca
     2581 gcccgactac tccgaggaca cggcccggaa gatcgacgac gaggtcaacc gcatcgtcac
     2641 gacctcgtac gcgcgcggca agaagatcat cgtcgagcac cgcgcggcgc tcgaggagat
     2701 cgcgaagatg ctcctcgaga aggaatccct cgacggcgag gagatctacg cgatcatcaa
     2761 gcgcgtcacc ggccacgagg tcgcgaagag gccgcccgtg aagcccgagc cgccgaagcc
     2821 caccgcgccc gccaccgtcg cgcagaagga cgagggcgcc gcgccggccg ggggcctcat
     2881 cccagtccct gcctgaggtg gatttccatc tgacgcttcc ggggggccgc gagctcgcgc
     2941 tcggggccct gccgggcgtc atggcgatcc tgaacgtcac ccccgactcc ttcagcgacg
     3001 gcgggcgctt cgcgtcggcc gcggcagcgg ccgacgaggg tgcccgcctc gcggacctgg
     3061 gcgtcacgct cgtggacgtc ggaggagaga gcacccgtcc caaaggggcg acctacggag
     3121 cgggggctac ggagattttc ttggaaggtg aattgggcag ggtgctgccc gtcatctccc
     3181 gtctccgtgg gttgcgcccg tctctgccca tctccgtcga cacgcgccgc gtggaggtgg
     3241 cacgcgcggc gctggacgag ggcgcggacg tggtgaacgt cgtcaccggt ctgaacgtcc
     3301 cgaaggatct cctcgatctg gtggcgagcc gcggcgtccc gatcgtgctc aaccactgcc
     3361 gaggcacgcc ctcgaccacc ttcgaagaat cttccttcac cgatgtcatc gccgaggtgc
     3421 gcgccgacct gaacggcgcg cgcagccgcg cgctctcctc cggagtccgg gcagaaagtg
     3481 tcttcctcga ccccggcctc gggttcggca agacgccgga gcagaacttc gcccttctcg
     3541 gcgcgctgga ccggctcgcg ccgcccggcg cgccgctcgt cgtcggcgcg tcgcgcaagg
     3601 cgttcctcgg caccgtggcg ggatcgcccg tgtcggaccg cctccccgag tcgctcgcgg
     3661 ccgccgccgt ctgcgcggag gccgcgcgca cgcgccccgt cctcgtgcgc gtccacgacg
     3721 cc
//
LOCUS       DAOMFY010000339         3705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9689_length_3705_cov_6.928493, whole genome shotgun sequence.
ACCESSION   DAOMFY010000339 DAOMFY010000000
VERSION     DAOMFY010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9689_length_3705_cov_6.928493"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLB01_18140"
     CDS             <1..845
                     /locus_tag="PLB01_18140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ79275.1"
                     /translation="AIFGKFRTGAKQAIKYNEKNEGALLDLYDFYMADKNWEPALDAL
                     RKAAEVNPENRDKLVALGRKARAEGDLVHARQVFATAAAAAPKDTSILTEYASVLLQM
                     KDLDAALEPLTKAAAAEPDREIVRANLAAVLRRKGLWKEAEKEYREAIRSDPDYAPAL
                     TGLGGLLLERGQAAEAIEPLKKAVLRAPQSVDAAWALARAQRQTGALKEAADGLERPL
                     ALDAPELWNEAGAIAMERGRWDEAVIYFDKAISRDGTVPLYKANRDRAAAAAAFVKST
                     APSS"
     gene            907..1626
                     /locus_tag="PLB01_18145"
     CDS             907..1626
                     /locus_tag="PLB01_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="filamin/ABP280 repeat domain-containing protein"
                     /protein_id="HQQ79276.1"
                     /translation="MKRRAVGLVLVAAMLSAMPGPATPLPQVSATPTKTIVLSVNGPA
                     TSTVLCNLLVLPSSVAGEEATFIIQARDASNHKRTAGGDTFVARLAGPETVSVSITDR
                     RDGTYAGRYTALRSGPHTLSVMRGNLNILGSPFTVVVGSGITSASLSSLLVLPTVAAG
                     GTGTFLIQARNQFGVKRDTGGDVFVARLSGPATPPVSVADQHDGTYRATFLVSGAGTY
                     TVDVLFGGLPISGSPFRFEAR"
     gene            complement(1648..2274)
                     /locus_tag="PLB01_18150"
     CDS             complement(1648..2274)
                     /locus_tag="PLB01_18150"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQQ79277.1"
                     /translation="MSGGAGSSGDVPRTWLDDVRARLVRDWPERRLELERGPDAGPLR
                     EAAVLIPLFVRERALWTLFTKRTDLVEHHKGQISFPGGGKHATDANPWETAIRETEEE
                     IGVPRAGVKILGALPKLVTVTDFEISPFVGAIPYPTQFEPHPGEVESIIEVPVSYLLD
                     PMVVEERPVKWKGRDVMTLVYHYKGHAIWGATARILGDFLTALRGSDP"
     gene            complement(2271..3417)
                     /gene="prfB"
                     /locus_tag="PLB01_18155"
     CDS             complement(join(2271..3350,3352..3417))
                     /gene="prfB"
                     /locus_tag="PLB01_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152543888.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQQ79278.1"
                     /translation="MNEERIERLLELASSVSSLRGYFDVRQAGREKDELERKTGEPGF
                     WANALEAQDTLRKLKRAERILADDKDLKERQEELEILAEFLKSGEAVEKDADTAIATV
                     GPWARHKELTFKLTNDEDPMPALLEIHAGAGGTEAQDWADMLMRMYLRYGERQGWKSD
                     VLEVTYADDAGIKTATLRFEGEYAYGHLKAERGVHRLVRISPFDAAARRHTSFAAVYV
                     SPEIDDTIEVEINEKDLKIDTFRAGGKGGQHVNKTESAIRITHLPSGLVVQCQNERSQ
                     HKNKASAMKVLRSRLYQIALEEQQAKNDARAGTKMEIGFGSQIRSYVLAPYRLIKDHR
                     TNYEVGDVDRVLDGDLDGFIEAFLQTMVKKGEKAATDRPGFNAADPA"
     gene            complement(3414..>3705)
                     /locus_tag="PLB01_18160"
     CDS             complement(3414..>3705)
                     /locus_tag="PLB01_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79279.1"
                     /translation="LRAVETGRLLVRAAVTGISGAVEPDGRVLVSLPAGRKGAFSAPV
                     FRPRGAPPAAAVGDAILWVCTAGLLAGILRARFPSPRGPVASASPAAPRGNT"
BASE COUNT          547 a         1329 c         1252 g          577 t
ORIGIN      
        1 cggccatttt cggcaagttc cggaccggtg cgaagcaggc gatcaagtac aacgagaaga
       61 acgaaggcgc gctcctcgac ctctacgact tctacatggc ggacaagaac tgggagccgg
      121 cgctggacgc ccttcgcaag gcggccgagg tcaaccccga gaaccgcgac aagctcgtcg
      181 cgctcgggcg caaggcgcgc gccgagggcg acctcgtgca cgcgcgccag gtcttcgcga
      241 ccgccgccgc ggcggcgccg aaggacacgt cgatcctcac cgagtacgcg tccgtccttc
      301 tccagatgaa ggacctcgac gcggccctcg agcccctcac gaaggccgcc gccgccgagc
      361 cggaccgcga gatcgtccgc gcgaacctcg ccgccgtcct ccggaggaag ggcctctgga
      421 aggaagccga gaaggagtat cgcgaggcga tccggtccga cccggactac gcgcccgccc
      481 tcacgggtct cggcgggctc ctcctcgagc gcggccaggc ggccgaggcg atcgagccgc
      541 tgaagaaggc ggtcctccgc gcgccgcaga gcgtcgacgc cgcatgggcg ctcgcccgcg
      601 cgcagcgcca gacgggcgcc ctcaaggagg cggccgacgg gctcgaaagg ccgctcgcgc
      661 tcgacgcgcc cgagctctgg aacgaggcgg gcgcgatcgc gatggagcgg ggacgctggg
      721 acgaggcggt catctacttc gacaaggcca tctcgcgcga cggcaccgtt ccgctctaca
      781 aggcgaaccg cgacagggcc gcggccgccg cggcgttcgt caagagcacg gcgccctcgt
      841 cctaacgcag cccttcgacg gcgtacagcc gccgtccgga agtcctcaaa cgcctagaat
      901 cgacggatga aaaggcgcgc cgtcggactc gtcctcgtcg ccgcgatgct ttcggcgatg
      961 cccggtccgg cgacgccgct gccgcaggtg tcggcaacac cgacaaagac catcgttctt
     1021 tcggtcaatg gtccggcaac cagcaccgta ctctgcaacc tcctcgtcct gccgtcgtcc
     1081 gtcgcgggcg aagaggccac gttcatcatc caggcccggg atgcgtcgaa ccacaagcgc
     1141 actgcaggag gcgacacgtt cgtagcacga ctcgcggggc ccgaaacggt gtccgtgtcg
     1201 atcaccgacc ggagggacgg cacgtacgcc ggccggtaca ccgcgctgcg gtcgggtcct
     1261 catacgctgt ccgtcatgcg aggcaacctg aacatcctcg gcagtccctt taccgtcgta
     1321 gtcggatccg gaatcacgag cgcgtcgctg tcgagcctcc tcgtcctgcc gacggtggcc
     1381 gccggcggga ccggaacgtt tctgattcag gcccgcaatc agttcggggt caagcgcgac
     1441 acggggggcg acgtcttcgt cgcacgactg agcggcccgg cgaccccacc ggtttccgtc
     1501 gcggaccagc acgacggcac gtaccgagcg acgttcctcg tcagcggcgc agggacctac
     1561 acggtggacg tgctgttcgg cggcctgccg atctccggaa gcccgtttcg gttcgaggcg
     1621 aggtaacact cgccggaccc tctcgcccta ggggtcgctt ccccgcagcg ccgtcaggaa
     1681 gtcgccgagg atgcgcgcgg tcgcgcccca gatcgcgtgg cccttgtagt ggtagacgag
     1741 cgtcatgacg tcccgtccct tccacttcac gggccgctcc tcgacgacca tcgggtcgag
     1801 gaggtacgac acgggcacct cgatgatcga ctcgacttcg cccgggtgag gctcgaactg
     1861 cgtcgggtac gggatcgcgc cgacgaacgg cgagatctcg aagtccgtga cggtgacgag
     1921 cttcgggagc gcgccgagga tcttcacgcc cgcgcgcggc acgccgatct cttcctcggt
     1981 ctcgcggatc gccgtctccc acgggttcgc gtcggtcgcg tgcttgcctc cgcccggaaa
     2041 cgagatctgc cccttgtggt gctccacgag gtccgtccgc ttcgtgaaga gcgtccagag
     2101 cgccctctcg cgcacgaaga gcgggatgag gaccgcggcc tcccggagcg gacctgcgtc
     2161 cgggccgcgc tcgagctcga gcctgcgctc gggccagtcc cggacgaggc gggcccggac
     2221 gtcgtccagc caggtgcgcg gaacgtctcc gctgctgccg gcgccgccgc tcacgccggg
     2281 tcggccgcgt tgaagcccgg gcgatcggtg gcggccttct cgcccttctt gaccatcgtc
     2341 tggaggaacg cctcgatgaa tccgtcgagg tcgccgtcga ggacgcggtc cacgtccccg
     2401 acctcgtagt tcgtgcggtg gtccttgatg agccggtacg gcgcgaggac gtacgaacgg
     2461 atctgcgagc cgaagccgat ctccatcttc gtgcccgccc gcgcgtcgtt cttcgcctgc
     2521 tgctcctcga gagcgatctg gtagagacgc gagcgcagga ccttcatcgc ggaggccttg
     2581 ttcttgtgct gcgaccgctc gttctggcac tgcacgacga ggccgctggg caggtgcgtg
     2641 atccggatcg ccgattccgt cttgttgacg tgctgcccgc ccttgccgcc cgcgcggaac
     2701 gtgtcgatct tgaggtcctt ctcgttgatc tcgacctcga tcgtatcgtc gatctccggc
     2761 gagacgtaga cggcagcgaa cgacgtgtgc cgccgcgccg cggcgtcgaa cggcgagatg
     2821 cgcacgagcc ggtgcacgcc gcgctcggcc ttcaggtgcc cgtacgcgta ctccccctcg
     2881 aacctcagcg tggccgtctt gatcccggcg tcgtccgcgt aggtcacctc gaggacgtcc
     2941 gacttccagc cctgccgctc gccgtagcgg aggtacatcc gcatgagcat gtcggcccag
     3001 tcctgcgcct cggtgccgcc ggcgcccgcg tggatctcga ggagggccgg catcgggtcc
     3061 tcgtcgttcg tgagcttgaa cgtcagctcc ttgtggcgcg cccacggccc gaccgtcgcg
     3121 atcgcggtgt ccgcgtcctt ctcgacggct tcgccggact tgaggaactc cgccaggatc
     3181 tcgagctctt cctgccgctc cttcaggtcc ttgtcgtcgg cgaggatgcg ctcggcgcgt
     3241 ttcagcttcc ggagcgtgtc ctgtgcttcg agggcgttcg cccagaaccc gggctcgccc
     3301 gtcttccgct ccagctcgtc cttctcgcgt cccgcctggc ggacgtcaaa gatagccccg
     3361 gagggagctc acggacgacg cgagttcgag caggcgctca atgcgttctt cgttcacgtg
     3421 tttcctctcg gcgccgcggg ggacgcggac gccacgggac cccggggcga ggggaaccgc
     3481 gcgcgcagga taccggcgag gaggccggcg gtgcaaaccc agaggatcgc gtcgccgacg
     3541 gcggcggcgg gcggcgctcc gcgcgggcgg aagaccggcg ccgagaacgc gcccttgcgt
     3601 cccgccggga gcgagacgag gacgcgcccg tccggctcga cggcgccgct gatccccgtc
     3661 acggcggcgc gcacgagcag ccgccccgtc tccacggcgc gcagc
//
LOCUS       DAOMFY010000340         3687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9771_length_3687_cov_5.763767, whole genome shotgun sequence.
ACCESSION   DAOMFY010000340 DAOMFY010000000
VERSION     DAOMFY010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9771_length_3687_cov_5.763767"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1655
                     /gene="priA"
                     /locus_tag="PLB01_18165"
     CDS             <1..1655
                     /gene="priA"
                     /locus_tag="PLB01_18165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQQ79280.1"
                     /translation="VWCIAGSRVGGGRRPVPTRAQQDAIAAVAASVASRRAQEVLLDG
                     VTGSGKTEVYLNALEQAVAAGRRGILLVPEIALAPALVRRLVARFGDRVSLLHSGLTD
                     GERASEWERARRGDVDAVVGPRSAVFAPLPDLGLVVIDEAHDASYKQAESPRYDARDV
                     ARVRAKEEGATVVFGSATPSMELERAAREGRLPRLALPERPGARPRAAVEVVDLRGET
                     AREGDHGRVLFAARTVEILRRCFGRGEQAIALLNRRGFSPSLLCRACGEDFRCRNCSV
                     ARTYHRRNESLLCHYCGDDIRRPPKCPACGSDALQPVGFGTERLAERFAETFPGVSYA
                     VLDRDAAARRGGAAGVLMDFESGKSQALLGTQMVAKGHDFPNATALAVLDADALLSFP
                     DFRAAEKTFQLVTQAAGRVGRGDKPGIVAVQTSRPDHEAIRAAVLQDHVAFADAELRF
                     RKAFRYPPYTRLLLALFADADLARAHRAAGDAFTALLSSPVAAKVKILGPAPAPIERL
                     KGLWRYHLVLKAEKREAIAEAARVLASLPDPPKLDVDPQNLL"
     gene            complement(1652..2593)
                     /locus_tag="PLB01_18170"
     CDS             complement(1652..2593)
                     /locus_tag="PLB01_18170"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ79281.1"
                     /translation="MGESKSPAGTGLVCPACHGVLSASAAALACVGCDRSFPVDDGIA
                     DFAEGRYFEDHPDPSSLSPETLRGFENETSGRRTEDYYLPILRASGSRRVLDSGCGSG
                     EAVDTLSEAGFEAWGHDLSAFRKWQWRARAHRERLVVADGFRLPFPDGFFDAVISSGV
                     LEHIGVQEHGEPGIYEVAPLPDRDARRAEFLAGLVRVLAPGGTLYLDFPNGAFPIDFW
                     HGTSAGGARWHSPFEGFLPKVKEIAALAESLDPVCRVSVRSPHRRLRFNQVGGHWYGR
                     LFRAPVRAFYALTSLPGFTWLAGTPLNPYLVLELRRS"
     gene            2679..3629
                     /locus_tag="PLB01_18175"
     CDS             2679..3629
                     /locus_tag="PLB01_18175"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQQ79282.1"
                     /translation="MANEAQFEAPLERVRRRLDELQGFADSPAKEREVTKLREKLGKL
                     SREIYENLTPWQKTLVARHPNRPFTLDYVRVLVQDFVEWKGDRAFADDPAIVAGFGML
                     GDRSVAIVGHQKGRDTKEKIRRNFGMPRPEGYRKALRVMKLAEKFGKPVLTFIDTPGA
                     YPGLESEERGVSEAIARNLIEMAALRTPIVATVIGEGGSGGALGIGIGDAVLMLEHSV
                     YSVISPEGCAAILWKDQGKAKEAADALRLTASDCKALGVIDEVIPEPVGGAHADPVAA
                     IDAVGRAVSAQLDRLSGMSAQPLLDARYAKFRSMGAWEGA"
BASE COUNT          537 a         1320 c         1336 g          494 t
ORIGIN      
        1 gggtttggtg catcgctggg tcgcgcgtgg ggggcgggcg ccggccggtg cccacgcgcg
       61 cgcagcagga cgcgatcgcc gcggtggccg cgtccgttgc gtcgcgtcgc gcgcaggaag
      121 tcctcctcga cggagtcacg ggcagcggca agaccgaggt ctacctcaac gcgctcgagc
      181 aggcggtcgc cgcgggtcgg cgcggaatcc tgctcgtccc cgagatcgcg ctcgcgcccg
      241 cgctcgtgcg ccggctcgtc gcgcgcttcg gagaccgcgt gtcgcttctg cacagcggcc
      301 tgacggacgg cgagcgcgcg tccgagtggg agcgcgcgcg acggggcgac gtggacgccg
      361 tcgtcggacc gcgctcggcg gtcttcgcgc cgctgcccga cctcggcctg gtcgtgatcg
      421 acgaggcgca cgacgcctcc tacaagcagg ccgagtcccc gcgctacgac gcgcgcgacg
      481 tggcccgcgt gcgggcgaag gaagagggcg cgacggtcgt cttcggctcc gcgacgccgt
      541 cgatggagct cgagcgcgcc gcacgggagg gccggctccc gcgcctcgct ctcccggagc
      601 gcccgggcgc gcgtccgcgc gcggccgtgg aggtcgtcga tctgcgcggc gagacggcgc
      661 gggagggcga ccacgggcgc gtccttttcg cggcgcgcac cgtcgagatc ctccgcaggt
      721 gcttcggccg cggcgagcag gcgatcgcgc tgctgaaccg gcggggcttc tcgccgtcac
      781 tcctctgccg ggcgtgcggc gaggacttcc gctgccgcaa ctgctcggtc gcgcggacgt
      841 accaccgtcg caacgagagc ctgctctgcc actactgcgg cgacgacatc cgccggcccc
      901 cgaagtgccc cgcgtgcggg tcggacgcgc tgcagcccgt cgggttcggg acggagcgcc
      961 tggccgagcg tttcgccgag acctttccgg gagtctccta cgccgttctc gaccgcgacg
     1021 ccgccgcccg gcgcggcggc gcggcgggcg tcctcatgga cttcgagtcc ggcaagtccc
     1081 aggcgctcct cgggacgcag atggtcgcca agggccacga cttcccgaac gcgacggcgc
     1141 tcgcggtgct cgacgcggat gctctcctgt ccttcccgga ttttcgcgcc gccgagaaga
     1201 ccttccagct cgtgacgcag gccgcggggc gcgtcgggcg cggcgacaag ccgggcatcg
     1261 tcgcggtcca gacgtcgcgc ccggaccacg aggcgatccg cgcggcggtc ctgcaggacc
     1321 acgtggcgtt cgcggacgcc gagctgcgct tccgcaaggc gttccggtac ccgccgtaca
     1381 cgcgtctcct cctcgcgctc ttcgcggacg cggacctcgc cagggcgcac cgggcggcgg
     1441 gggacgcctt cacggcgctc ctgtcgtcgc cggtcgcggc gaaggtcaag atcctcgggc
     1501 cggcgcccgc cccgatcgag cgcctcaagg gcctctggcg ctaccacctc gtcctgaagg
     1561 ccgagaagcg cgaggcgatc gcggaagccg cgcgcgtcct cgcgtcgctg cccgacccgc
     1621 cgaagctgga cgtcgacccg cagaatctgc tctaggagcg ccggagctcg aggaccaggt
     1681 acgggttcag cggcgtccct gcgagccacg tgaagccggg cagcgaggta agcgcataga
     1741 acgctctcac cggagcgcgg aagaggcgcc cgtaccagtg gccgccgacc tggttgaaac
     1801 gaagcctgcg atgcggggac cggaccgaga cgcggcagac ggggtcgagc gattctgcga
     1861 gagcggcgat ctccttcacc ttcggaagaa atccttcgaa gggtgaatgc caccgggcgc
     1921 cgccggcgct cgtcccgtgc cagaagtcga ttgggaacgc tccgttggga aaatccagat
     1981 acagagtgcc gccgggggcg agaacgcgga cgagacccgc gagaaattca gcgcgacgcg
     2041 cgtcccggtc tggcagcgga gcgacctcgt agatccccgg ctctccgtgc tcctggactc
     2101 cgatgtgctc gaggactccg gaggaaatca cggcgtcgaa gaagccgtcc ggaaagggga
     2161 gacggaaccc gtctgcgacc acgaggcgct cgcggtgcgc ccgcgcccgc cactgccact
     2221 tgcgaaacgc ggagaggtcg tgcccccacg cctcgaagcc cgcctccgag agcgtgtcga
     2281 cggcctcgcc gctcccacag cccgagtcga ggacgcgccg cgagccggag gcgcggagga
     2341 tcgggaggta gtaatcctct gtgcggcgac ccgatgtttc gttctcgaag cctcggagcg
     2401 tctccgggga cagggaggaa ggatcggggt ggtcttcgaa gtagcgcccc tccgcgaagt
     2461 cggcgattcc gtcgtcgacc gggaatgagc ggtcgcagcc cacgcacgcg agcgcggcag
     2521 ccgacgccga gagcacgccg tggcacgccg ggcagaccag accggttccc gcgggggact
     2581 tcgactctcc cactcgaaga atttctcctc cttcggagct tgccccgaat cgccggcgcc
     2641 gtcgagcgag ggaaagagcc ctctgctaca gtctccccgt ggcgaacgag gcccagttcg
     2701 aagcgcccct ggagcgcgtc cggcgccggc tggacgagct gcagggcttc gcggacagcc
     2761 cggcgaagga gcgcgaggtc acgaagctcc gcgagaagct cgggaagctc tcgcgcgaga
     2821 tctacgagaa cctgacgccc tggcagaaga cgctcgtcgc gcggcatccg aaccggccgt
     2881 tcacactcga ttacgtccgc gtcctcgtcc aggacttcgt cgagtggaag ggcgaccgag
     2941 cgttcgcgga cgacccggcc atcgtcgcgg gcttcgggat gctgggggac cgctccgtcg
     3001 cgatcgtcgg ccaccagaag ggccgcgaca cgaaggagaa gatccgccgc aacttcggga
     3061 tgccgcgccc cgagggctac cgcaaggcgc ttcgcgtcat gaagctcgcc gagaagttcg
     3121 ggaagcccgt cctcacgttc atcgacacgc cgggcgcgta tcccggcctc gagagcgagg
     3181 agcgcggcgt ctccgaggcg atcgcgcgga acctcatcga gatggccgcg ctccggacgc
     3241 cgatcgtcgc gaccgtgatc ggcgagggag gctccggcgg ggcgctcggg atcggcatcg
     3301 gcgacgccgt cctgatgctc gagcactccg tctactcggt catctcgccc gagggctgcg
     3361 ccgcgatcct ctggaaggac caggggaagg cgaaggaggc cgcggacgcc ctccgcctca
     3421 cggcctcaga ctgcaaggct ctcggcgtga tcgacgaggt catccccgag cccgtcggcg
     3481 gcgcgcacgc ggaccccgtc gcggcgatcg acgccgtcgg ccgcgcggtg tccgcgcagc
     3541 tggaccgcct gtccgggatg tccgcgcagc cgctcctcga cgcccgctac gcgaagttcc
     3601 gttcgatggg ggcctgggaa ggcgcgtgag cgcgcgggtc cgtgccccgc gcctcgtccg
     3661 ccgccgcctg cggacgcaga accccga
//
LOCUS       DAOMFY010000341         3662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9885_length_3662_cov_8.015525, whole genome shotgun sequence.
ACCESSION   DAOMFY010000341 DAOMFY010000000
VERSION     DAOMFY010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9885_length_3662_cov_8.015525"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLB01_18180"
     CDS             <1..487
                     /locus_tag="PLB01_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQQ79283.1"
                     /translation="EAKFPLLPPGCTAHLIGPVQSNKANRAAKVAAVVQTIDSEDLAR
                     RLDRAAAALGKRLGAFVQVRLGGEATKSGVEPGEAPALAAAVRALPNLDLQGLMAIPP
                     PGDTRPHFVALRRLGESLGLAGLSMGMSDDFEAAVEEGATLVRVGSAIFGARETKKEI
                     S"
     gene            502..1185
                     /locus_tag="PLB01_18185"
     CDS             502..1185
                     /locus_tag="PLB01_18185"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF016400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQQ79284.1"
                     /translation="MRPTYQTRFATPPGWRRIPPVTRALLVLVAAGYLLTLAWPVAAA
                     WLVLAPGAVLAGQVWRLLTYAVVGASISGVLFSLLLVWSLGSEMEPRWGSRRFALFLV
                     AAAAAAGVLGLFVGGGAGLAPVLLALIFAWMLEGPSQSLLFFGVFPMTRLAFAALALI
                     LVVFSELEATRSLLRLLFVLGGLPVAWLFARGFGGGRGGGPRFPRIRNPFRRRRFTVV
                     DSGSDFRVH"
     gene            1215..1730
                     /locus_tag="PLB01_18190"
     CDS             1215..1730
                     /locus_tag="PLB01_18190"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HQQ79285.1"
                     /translation="MRPTISSLEIRKRQFPKKFRGVDPAEVSQFLEALAEDFEEMSRE
                     LDELTRENARLRDENMRHRETEATLKETLLLAQRSADSLRETSEKDAGRILGEAETKA
                     EKMVQQAMDRMAETEKKIRELRMERRNFHIKFQGMLDLFQQVLKFDKEEDEMEASVSV
                     HRPKRKEGEAG"
     gene            1838..3007
                     /gene="hutI"
                     /locus_tag="PLB01_18195"
     CDS             1838..3007
                     /gene="hutI"
                     /locus_tag="PLB01_18195"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQQ79286.1"
                     /translation="MRFENGRIAFAGTDTEFVRRYGEAPLPGETILDGRGKTAVPGFV
                     DAHTHLPWDGFREDEFNRRLKGETYAGIAAAGGGIVATVAATRAASVETLAANVRVRL
                     DRMLRHGTTFCEAKTGYGLDLANETKQLAAILAGAKGHPVGVAPTALPAHELPPERRG
                     SSALRRLYVDECVNEILPALAEGGARFADVFCEEGVFSLAESAEILEAGRSLGLVPRV
                     HADELTPLGGASLAARLRAASADHLLFATDAGITDMASAGVVATLLPGTSVFLRMNRY
                     APARKMVAAGVAVALGTDCNPGSSYTESLPAVAFLASVGMGLTVEETLTAMTLNAAAS
                     LGEGVRRGTLEEGKAGDAVLLDGPALEHLVYHWGVSLVTDVIAGGRHVVQRGLLR"
     gene            3012..3650
                     /locus_tag="PLB01_18200"
     CDS             3012..3650
                     /locus_tag="PLB01_18200"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HQQ79287.1"
                     /translation="MSRLASLTVEGFAAALASDAPAPGGGSAAAAGGANGAALLAMVV
                     RLTLGKEKYKSAWEELAPLVDRLDDVRAKLVELIDEDTKAFDAVLAARRLPKETDADK
                     AARQKAIDEANVLATTVPMQTAFFAHEALKNAPAILEKGNPNAASDAWAGALASWAAV
                     LAALANVRINLPGVADPELLRGFREDADLMEKTAGEALEKTRALARSRGLAA"
BASE COUNT          497 a         1353 c         1295 g          517 t
ORIGIN      
        1 ggaggcgaag ttcccgctcc tcccgccggg ctgcacggcg catctcatcg gccccgttca
       61 gtccaacaag gcgaaccgcg ccgcgaaggt cgcggccgtc gtccagacga tcgactccga
      121 agacctcgcg cgccgcctcg accgcgcggc ggcggcgctc ggcaagcgcc tcggcgcgtt
      181 cgtccaggtg cgcctcggcg gcgaggccac gaaatccggc gtcgagcccg gagaggcccc
      241 ggcgctcgcg gccgccgtgc gtgcccttcc gaacctcgac ctccagggac tcatggcgat
      301 cccgccgccc ggcgacacgc gtccgcactt cgtggcgctg cgccgcctcg gagagtcgct
      361 cggcctcgcc ggcctctcca tggggatgag cgacgacttc gaggccgccg tcgaggaggg
      421 cgcgacgctc gtccgcgtcg gctcggcgat cttcggggca cgagagacga agaaggagat
      481 ctcttagaag gtgaagagag gatgaggccc acctaccaga ctcgcttcgc aacgccgccg
      541 ggctggcggc ggatcccgcc cgtgacgcgc gcgctcctcg ttctcgtcgc ggcgggctac
      601 ctcctgaccc ttgcgtggcc ggtcgcggcc gcgtggctcg ttctggcgcc gggcgccgtc
      661 cttgccgggc aggtctggcg ccttctcacc tacgccgtcg tgggcgcgtc gatctcgggc
      721 gttctcttct cgctcctgct cgtgtggagc ctcggcagcg agatggagcc gcgctggggc
      781 tcgcggcggt tcgcgctgtt cctcgtggcc gccgcggcgg ccgccggcgt tctcggcctc
      841 ttcgtcggag gcggggcggg gctcgcgccg gtgctcctcg cgctgatctt cgcgtggatg
      901 ctcgagggac cctcgcagag cctcctgttc ttcggcgtct tcccgatgac ccggctcgcc
      961 ttcgccgcgc tcgccctgat cctcgtcgtc ttctccgagc tcgaggcgac gcgctccctc
     1021 ctccgcctcc tcttcgtcct cggcgggctg ccggtcgcgt ggctcttcgc gcgcggattc
     1081 ggcggcggaa ggggcggcgg tccgcgcttt ccgcggattc ggaatccgtt ccggcgccgg
     1141 cgcttcaccg tcgtcgactc cgggtccgac ttccgcgtgc attaggttga ccccaaaggg
     1201 tttataatcc gtccatgcgg ccaaccatca gctctctcga gatccggaaa cggcagttcc
     1261 cgaagaagtt ccgcggcgtg gacccggcag aggtgtcgca gttcctcgaa gctctcgcgg
     1321 aggatttcga ggagatgtcg cgcgagctgg acgagctcac gcgcgagaac gcgcgcctca
     1381 gggacgagaa catgcgccac cgggagacgg aggcgacgct gaaggagacg ctcctcctcg
     1441 cccagcgctc ggccgacagc ctgcgcgaga cgagcgaaaa ggacgccggc cgcatcctcg
     1501 gcgaggccga gacgaaggcc gagaagatgg tccagcaggc gatggaccgc atggccgaga
     1561 cggagaagaa gatccgcgag ctccgcatgg agcgccgcaa cttccacatc aagttccagg
     1621 ggatgctcga cctcttccag caggtcctga agttcgacaa ggaggaggac gagatggagg
     1681 cctccgtctc cgtccaccgt ccgaaacgga aggaagggga ggccggctga ccggcgtcct
     1741 caccgtccgg cgcctcgcgg agcttgcgac gccggaggga accgccgccc gctgcggcgc
     1801 cgcgcagggc gcgatccggc gcgtcacgag cgcggccctg cgcttcgaga acgggcgcat
     1861 cgcgttcgcc ggcacggaca cggagttcgt gaggcgctac ggcgaggcgc cgctgcccgg
     1921 cgagacgatc ctcgacggcc gcggcaagac cgccgtcccc ggtttcgtcg acgcgcacac
     1981 ccacctgccg tgggacggtt tccgcgagga cgagttcaac cggcggctca agggcgagac
     2041 gtacgcaggc atcgcggccg cggggggcgg catcgtcgcg acggtcgcgg ccacgcgcgc
     2101 ggcctccgtc gagacgctcg cggcgaacgt gcgcgtgcgc ctcgaccgca tgctccggca
     2161 cggcacgacg ttctgcgagg cgaagaccgg ctacggcctc gacctcgcga acgagacgaa
     2221 gcagctcgcc gcgatcctgg ccggcgcgaa gggccacccc gtcggcgtcg cgccgaccgc
     2281 gctgcccgcg cacgagctgc cgcccgaacg ccggggctcg tccgccctcc ggaggctcta
     2341 cgtcgacgaa tgcgtgaacg agatcctccc ggcgctggcg gaaggcggcg cccgcttcgc
     2401 ggacgtcttc tgcgaggagg gcgtcttctc gctcgcggaa tcggccgaga tcctcgaggc
     2461 ggggcggtcc ctcggtctcg tcccccgcgt ccacgccgac gagctgacgc cgctcggcgg
     2521 agcgtcgctc gcggcgcggt tgcgcgccgc ctccgccgac cacctcctct tcgcgacgga
     2581 cgcgggcatc acggacatgg cctcggccgg cgtcgtcgcg acgctcctgc cgggcacgtc
     2641 cgtgttcctg cgcatgaacc ggtacgcgcc ggcccggaag atggtcgccg ccggcgtcgc
     2701 cgtcgccctc ggaaccgact gcaaccccgg gtcgtcctac accgagtcgc tcccggccgt
     2761 ggcgttcctc gcgtcggtcg gcatgggcct cacggtggag gagaccctca ccgcgatgac
     2821 gttgaacgcc gccgcctcgc tcggcgaagg cgtgcgccgc ggcacgctcg aggaagggaa
     2881 ggcgggcgac gcggttctcc tcgacggccc ggccctggag cacctcgtct atcactgggg
     2941 cgtcagcctc gtgacggacg tgatcgcggg cgggcgccac gtcgtccagc gcggcctgct
     3001 acgctgaagg catgagccgc ctcgcctccc tcaccgtcga aggtttcgcc gccgctctcg
     3061 catccgacgc gcccgccccg ggcggcgggt cggccgccgc cgccggaggg gcgaacggcg
     3121 ccgcgctcct cgcgatggtc gtccgcctga cgctcggcaa ggagaagtac aagtccgcgt
     3181 gggaggagct cgccccgctc gtcgaccggc tggacgacgt gcgcgcgaag ctcgtcgagc
     3241 tcatcgacga agacacgaag gcgttcgacg ccgttctcgc cgcccgccgc cttccgaagg
     3301 agacggacgc cgacaaggcc gcgcgccaga aggcgatcga cgaggcgaac gtcctcgcga
     3361 cgaccgtgcc gatgcagacg gcgttcttcg cgcacgaggc cctcaagaac gcgccggcga
     3421 tcctcgagaa ggggaatccg aacgcggcgt ccgacgcctg ggccggcgcg ctcgcctcgt
     3481 gggcggccgt cctcgcggcg ctcgcgaacg tgcggatcaa cctgcccggc gtcgccgacc
     3541 ccgagctcct gcgcgggttc cgcgaggacg ccgacctcat ggagaagacg gcgggcgagg
     3601 cgctcgagaa gacgcgcgcg ctcgcccgct cccgcggtct cgccgcgtga cgccgcggcc
     3661 cg
//
LOCUS       DAOMFY010000342         3645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_9977_length_3645_cov_5.263231, whole genome shotgun sequence.
ACCESSION   DAOMFY010000342 DAOMFY010000000
VERSION     DAOMFY010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9977_length_3645_cov_5.263231"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..2809
                     /gene="mutS"
                     /locus_tag="PLB01_18205"
     CDS             182..2809
                     /gene="mutS"
                     /locus_tag="PLB01_18205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQQ79288.1"
                     /translation="MRATPMLEQYWALKREAGDAILLYRLGDFYEMFFEDAEIAAPLL
                     GLVLTARHKDSDIEAPMCGIPQHALESYLARLVGAGKKVAISEQTEAAAKGRALVARK
                     IVRVVTPGTIVDPDRLDARRANEMAAVAWDGAGAAVSCLDLSTGDFSVLPLRDAGGVS
                     EWLLRRAPRELVVFPSDRERAESLLGALRSGWADPPALSVLASESPRGASAADLLKRH
                     FRTATLEPFGLGERGPSADAAAALLSYARATQRADCDHVRSLRRESEGDGLVVDSVTA
                     GHLELFRSARDGSADGTLVSLMDRTGTAFGARTLRRILERPLGRIPEIEGRLDAVEEL
                     TADAARLEALAKGFREIPDLPRLVSRLAVGAGTPRDAALLASGLVRAARLSGTLDGVS
                     CPLLAPGGDASPDGVPFGLGESVSARLVPEPPLSAKDGGLFRDGVDAQVDEIRTLRRE
                     SATVLAALEAGEKAARGIPTLRVKFNQVFGHVFEVPGAARAKIPENALKRQTLANVER
                     YATPALVEVDEKLRSADARLAARELELFQQLVAEVVVESGRILGAAARLGLLDALVSF
                     ARVARAAGWTRPRLVEEPVLQVEGGRHPVVEALRPREPFVPNDASLGGEGAHVVVLTG
                     PNMGGKSTYLRQNAILVLLAHAGSFVPAASARVGLCDRIFTRVGASDSLVRGESTFLV
                     EMAETAHILRHATERSLVILDEIGRGTSTFDGLSLAWAIAEHLHDTGARRVLFATHYH
                     ELTEMALVKPDVVNRTMTAKEWNGEVVFLRKVSDGTADRSYGVQVARLAGIPEPVLVR
                     AREILGNLERHQLDVGGRPVLAEHAGAPPQKNAQLDLFRGQGEIVLDTIGKADVDSMT
                     PLAALQLLASLQHRLRGED"
     gene            2899..>3645
                     /locus_tag="PLB01_18210"
     CDS             2899..>3645
                     /locus_tag="PLB01_18210"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQQ79289.1"
                     /translation="MGGASLSAEERAFFAAHPPLGFLLFRRNLQTPSQTVELLEALRR
                     LSSPEPLLFLDQEGGTVDRLGPLLGVPFASAAACAEAGTDRIHENAYLMGRAARLLGF
                     DVDFAPCLDLGQPGAGAVVLEGRCFGFHAEDVVIGGMVFLHGLARAGLATCVKHFPGL
                     GRGAVDSHAKLPVVDAHDVDLMVTDVAPFTKLARGADGVMVGHAAYPGFEDGAIVPAS
                     CSPKIHSILRGPVRFDGVVYSDDLEMSALEG"
BASE COUNT          480 a         1337 c         1296 g          532 t
ORIGIN      
        1 ccccggcgag ggcggcggcg acggcgttcg ggatcgcctc ggcgcccgcg gcgggcagcg
       61 cggtcgccgg gccgaggccc gtctcgtccg cgaccgccag cgcgggtccg ggagaggccg
      121 aatcgagcga gagcacgcgc atcaacgggg cttcactcga tgctagcatc cccgacctcc
      181 gatgcgcgcg acgcccatgc tcgagcagta ctgggcactg aagcgggaag cgggcgacgc
      241 gatcctcctc taccgcctcg gggacttcta cgagatgttc ttcgaggacg ccgagatcgc
      301 ggcgccgctg ctcggcctcg tgctcacggc acgccacaag gactcggaca tcgaggcccc
      361 gatgtgcggg atcccgcagc acgccctcga gtcgtacctc gcccgcctcg tcggcgcggg
      421 gaagaaggtc gcgatctccg agcagaccga ggcggccgcc aagggccgcg ccctggtcgc
      481 gcggaagatc gtccgcgtcg tgacgccggg gacgatcgtc gaccccgacc ggctcgacgc
      541 gcggcgcgcg aacgagatgg cggccgtcgc ctgggacggc gccggagcgg ccgtgtcctg
      601 cctcgacctg tcgacgggcg acttctccgt cctcccgctg cgcgatgcgg gcggagtgtc
      661 cgaatggctg ctccggcgcg cgccgcgcga gctcgtggtc ttcccgtccg accgcgagcg
      721 ggccgagtcg cttctcgggg ccctgcgctc cggctgggcg gatccgcccg cgctctcggt
      781 cctcgcttcg gagtctccgc gcggcgcgag cgccgcggat ctcctgaaac ggcacttccg
      841 gaccgcgacg ctcgaaccct tcggcctcgg cgagcgcggc ccgtcggcgg acgccgcggc
      901 ggcgctcctc tcgtacgcgc gcgcgacgca gcgcgccgat tgcgaccacg ttcgatcgct
      961 gcggcgcgag tccgagggcg acggtctcgt cgtcgattcc gtgaccgcgg ggcacctcga
     1021 gctcttccgt tcggctcggg acggaagcgc ggacggcacg ctcgtgtcgc tcatggaccg
     1081 gacgggcacg gcgttcggtg cgcgcacgct gcgccgcatc ctcgagcgcc cgctcgggag
     1141 gatccccgag atcgagggcc ggctggacgc cgtcgaggag ctcacggccg acgcggcgcg
     1201 cctcgaggcg ctcgcgaagg gcttccgcga gatccccgac ctgccgcgcc tcgtctcgcg
     1261 cctcgcggtg ggcgcgggca cgccccgcga cgccgctttg ctcgcgtccg ggctcgtccg
     1321 ggcggcccgc ctctccggga cgctcgacgg cgtctcctgc ccgctcctcg cgcccggcgg
     1381 cgatgcctcg cccgacggcg tgccgttcgg cctcggcgag tccgtgtccg cgcgcctcgt
     1441 tcccgagccg ccgctctcgg ccaaggacgg cggactcttc cgcgacggtg tggacgcgca
     1501 ggtagacgag atccgcacgc tgcggcgcga gtcggcgacc gtcctcgccg cgctcgaagc
     1561 cggcgagaag gccgcacgcg ggatcccgac gttgcgcgtg aagttcaacc aggtcttcgg
     1621 ccacgtcttc gaggtgccgg gcgccgcccg cgcgaagatt cccgagaacg cgctcaagcg
     1681 ccagacgctc gcgaacgtcg agcgttacgc gacgcccgcc ctcgtcgagg tggacgagaa
     1741 gctccgctcg gccgacgcgc ggctcgccgc acgcgagctc gagctcttcc agcagctcgt
     1801 cgccgaggtc gtcgtcgagt cggggcgcat cctcggcgcg gccgcgcggc tcggcctcct
     1861 ggacgcgctc gtctccttcg cccgcgtcgc gcgcgccgcg ggctggacgc ggccacgcct
     1921 cgtcgaggag ccagtgctcc aggtggaggg cggccgccac ccggtcgtcg aggcactccg
     1981 cccgcgcgag ccgttcgtcc cgaacgacgc gagcctcggc ggcgaggggg cgcacgtcgt
     2041 ggtcctcacg ggcccgaaca tgggcggcaa gtccacgtac ctgaggcaga acgcgatcct
     2101 cgtcctcctc gcgcacgcgg gctcgttcgt gccggcggcg tcggcgcgag tcggactctg
     2161 cgaccgcatc ttcacgcgcg tcggcgcctc agactcgctc gtccgcggcg agtcgacgtt
     2221 cctcgtcgag atggccgaga cggcccacat cctgaggcac gcgaccgaga ggagtctcgt
     2281 gatcctcgac gagatcgggc gcgggacgtc gacgttcgac ggcctctcgc tcgcctgggc
     2341 gatcgccgag cacctccacg acaccggggc gcgccgcgtg ctcttcgcga cccactacca
     2401 cgagctgacc gagatggcgc tcgtgaagcc ggacgtcgtg aaccgcacga tgacggcgaa
     2461 ggagtggaac ggcgaggtcg tgttcctccg gaaggtgagc gacggcacgg cggaccgctc
     2521 gtacggcgtg caggtcgcgc ggctcgccgg catcccggag cccgtcctcg tgcgggcgcg
     2581 cgagatcctc ggcaacctcg agcgccacca gctcgacgtc ggcggacgcc ccgtcctcgc
     2641 cgagcacgcg ggagcgccgc cgcagaagaa cgcgcagctc gacctcttcc gcgggcaggg
     2701 ggagatcgtg ctcgacacga tcggcaaggc cgacgtggac tcgatgacgc cgctggcggc
     2761 gcttcaactt ctcgcttctc tccagcaccg cctcagggga gaagattgaa gaaagaaaaa
     2821 gaaagagaga agagaagatt ttcttagaag gtgaagaggg ccgtggctga cgcctccgcc
     2881 tcgcggttcg tgttcggggt gggcggggcc agcctgtccg cggaagagcg tgcgtttttc
     2941 gccgcgcacc ctcccctcgg cttcttacta ttcagaagaa atcttcaaac accctctcaa
     3001 actgtcgaac ttctggaggc gttgagaagg ctctcttctc cggagcctct tctgtttctg
     3061 gaccaggaag gcgggaccgt cgaccgcctc ggcccgctcc tcggcgttcc gttcgcctcc
     3121 gccgccgcct gcgcggaggc cgggacggac cggatccacg agaacgccta cctcatgggg
     3181 cgcgccgcgc ggctcctcgg gttcgacgtg gatttcgcgc cgtgcctcga cctcgggcag
     3241 cccggcgcgg gcgcggtcgt cctcgaaggc cggtgctttg gcttccacgc cgaggacgtc
     3301 gtgatcggcg ggatggtctt cctgcacggc ctcgcccgcg cgggactcgc gacgtgcgtc
     3361 aagcacttcc cgggcctcgg gcgtggggcc gtggactcgc acgcgaagct gcccgtcgtc
     3421 gacgcccacg acgtcgacct catggtcacg gacgtcgccc cgttcacgaa gctcgcgcgc
     3481 ggcgcggacg gcgtcatggt gggccacgcg gcgtatcccg ggttcgagga cggcgcgatc
     3541 gtgcccgcgt cgtgctcgcc gaagatccac tcgatcctga gggggcccgt ccggttcgac
     3601 ggcgtcgtct actcggacga cctcgagatg agcgcgctcg agggc
//
LOCUS       DAOMFY010000343         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10057_length_3627_cov_8.630459, whole genome shotgun sequence.
ACCESSION   DAOMFY010000343 DAOMFY010000000
VERSION     DAOMFY010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10057_length_3627_cov_8.630459"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PLB01_18215"
     CDS             complement(<1..1022)
                     /locus_tag="PLB01_18215"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQQ79290.1"
                     /translation="MAQFVWKGRTRQGATTGGVLVADSKDLAIADLRRRQIDVTTVKE
                     KGKELAVPKLGPGKVNARRLAIFTRQFSVMIDAGLPLVQCLEILGNQQDSRLFQKIIL
                     AVRQDVEAGSSLAEAMRKHPKAFDDLFVNMVAAGEAGGILDTILRRLSTYIEKAVKLK
                     GQVKTALIYPVSVLLVAAIVVFIILWKVIPTFASLFAALGAELPLPTRLVIKASNFVA
                     SYIVFIVAGVAALVYALKRYYATYRGRRVIDGIVLKLPVLGDIMKKIAVARFCRTLAT
                     LTSSGVPILDGLEITAKTAGNAIVEDAIMGVRKSVEAGRTIAEPLGETKVFPPMVVQM
                     IGVGEQT"
     gene            complement(1038..2177)
                     /locus_tag="PLB01_18220"
     CDS             complement(1038..2177)
                     /locus_tag="PLB01_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQQ79291.1"
                     /translation="MAVTLHQLLKTLVERGGSDLHITTNSPPMIRVDGDLIPLDYPPL
                     TVPETKQLAYSILTDVQKHRFEEQLELDLSFGVKGLARFRGNCFLQRGAVSAVFRQIP
                     YEILGFRELGVPNVVETLCNKPRGLVLVTGPTGSGKSTTLATMIDKINREQPRHIVTI
                     EDPIEFLHTHKRCIVNQRELHADTHSFPNALRAVLREDPDVVLVGEMRDLETIECALR
                     IAETGHLTLATLHTNSAVQTMNRVIDVFPASQQPQVRAQLSFVLEGILCQSLLPRASG
                     KGRCMAMEILVPTPAIRNLIREDKLHQVYSMMQTGQNKYGMQTFNQSLATLVHRRVIS
                     QELAMTVSSLPDELTEMIARGAGVVTSTAAGGNPPPPPANAVRRA"
     gene            complement(2252..3193)
                     /gene="nhaR"
                     /locus_tag="PLB01_18225"
     CDS             complement(2252..3193)
                     /gene="nhaR"
                     /locus_tag="PLB01_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HQQ79292.1"
                     /translation="MTDMIGPRLEWINYHHLLYFWLVAREGGLVPAGKVLRLSHPTLS
                     AQIHALEDRLGEKLFAKEGRKLALTEVGRIVFGYADEIFTLGREMVDTVRDRATGRPL
                     RLEVGVVDVVPKVVVRRLLEPALALPEPVRIVCREDSYERLLADLALHTLDVVIADAP
                     VPSGSRVRAFHHLLGETGVSFFAAPALARAVRRGFPRSLHGAPVLLPLESSVLRRTLD
                     HWFDRRGIRPRVVAEFQDSALLKVFGADGLGVFTAPSAVEEEVCRQYGVKVVGAAPDV
                     KERFYAVSVERRLRNPAVVAISDAARHEIFAGGARRS"
     gene            3294..>3627
                     /locus_tag="PLB01_18230"
     CDS             3294..>3627
                     /locus_tag="PLB01_18230"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQQ79293.1"
                     /translation="MKIDVRFRGIQVSEALREYAVRRVRFQLSRFDGDVGSVVVRIGD
                     VNGPRGGVDKRCLVEARGPAFGPVAVEDASTDAYTAVGSAVGRAARAVGREIERARVG
                     QREDGPVGE"
BASE COUNT          555 a         1171 c         1346 g          555 t
ORIGIN      
        1 gtctgctcgc cgacgccgat catctggacg accatcggcg ggaagacctt cgtctcgccg
       61 agcggctcgg cgatcgtgcg gcccgcctcg accgacttgc gcacgcccat gatcgcgtcc
      121 tcgacgatcg cgtttccggc cgtcttcgcc gtgatctcga ggccgtcgag gatcgggacg
      181 cccgacgacg tgagggtcgc gagggtgcgg cagaaacgcg cgacggcgat cttcttcatg
      241 atgtcgccga ggaccggcag cttgaggacg atcccgtcga tcacgcgccg gccgcggtac
      301 gtcgcgtagt agcgcttgag ggcgtagacg agggccgcga cgcccgcgac gatgaagacg
      361 atgtagctcg cgacgaagtt cgaggccttg atgacgaggc gcgtcgggag cgggagctcc
      421 gcgccgaggg ccgcgaagag ggacgcgaac gtcgggatga ccttccagag gatgatgaag
      481 acgacgatcg ccgcgacgag caggaccgag acggggtaga tgagcgccgt cttgacctgt
      541 cccttcagct tgacggcctt ctcgatgtag gtcgagaggc ggcggaggat cgtgtcgagg
      601 ataccgcccg cctcgccggc ggcgaccatg ttcacgaaga ggtcgtcgaa cgccttcggg
      661 tgcttgcgca tcgcctcggc gagcgacgag cccgcctcga cgtcctggcg cacggcgagg
      721 atgatcttct ggaagaggcg gctgtcctgc tggttgccca ggatctcgag gcactgcacg
      781 agcggaaggc ccgcgtcgat catgaccgag aactggcgcg tgaagatcgc gaggcgccgc
      841 gcattgacct tgccgggccc gagcttcggg acggcgagct ccttgccctt ctccttgacc
      901 gtcgtgacgt cgatctggcg gcggcggagg tccgcgatcg cgaggtcctt cgagtcggcg
      961 acgaggacgc cgccggtcgt ggcgccctgg cgcgtgcggc ctttccagac gaactgagcc
     1021 atcgggtccc tcctggatca ggcgcgccgc acggcgttcg ccggcggagg cggtgggttc
     1081 ccgccggcgg ccgtcgacgt cacgacgccg gcgccgcggg cgatcatctc ggtgagctcg
     1141 tccgggagcg aggagaccgt catcgcgagc tcctgggaga tcacgcggcg gtggacgagg
     1201 gttgcgagcg actggttgaa ggtctgcatg ccgtacttgt tctggccggt ttgcatcatc
     1261 gagtaaacct ggtgaagctt gtcctcgcga atgaggttcc ggatcgcggg cgtcgggacg
     1321 aggatctcca tcgccatgca gcgtcccttg cccgacgcgc gggggaggag gctctggcag
     1381 aggatcccct ccagcacgaa cgacagctgc gcgcgcacct gcggctgctg gctcgccggg
     1441 aagacgtcga tgacgcggtt catcgtctgg accgcgctgt tcgtgtggag cgtcgcgagc
     1501 gtgaggtggc ccgtctcggc gatgcggagg gcgcactcga tcgtctcgag gtcgcgcatc
     1561 tcgccgacga ggacgacgtc cgggtcctcg cggaggacgg cgcggagcgc gttcgggaac
     1621 gagtgcgtgt ccgcgtgcag ctcgcgctgg ttcacgatgc agcgcttgtg ggtgtggagg
     1681 aactcgatcg ggtcctcgat cgtcacgatg tgccgcggct gctcgcggtt gatcttgtcg
     1741 atcatcgtcg cgagcgtcgt ggacttgccc gagcccgtcg ggcccgtcac gaggacgagg
     1801 ccgcgcggct tgttgcagag cgtctcgacg acgttcggga cgccgagctc gcggaacccg
     1861 aggatctcgt aggggatctg gcggaagacg gccgagacgg cgccgcgctg gaggaagcag
     1921 ttgccgcgga agcgcgcgag gcccttcacg ccgaaggaga ggtcgagctc gagctgctcc
     1981 tcgaaccggt gcttctggac gtccgtcagg atcgagtaag cgagctgctt cgtctcgggg
     2041 acggtcagcg gcgggtagtc cagcggaatg aggtccccgt ccactcggat catcggggga
     2101 gagttcgtcg tgatgtggag gtcggacccg cccctttcga cgagcgtctt gaggagctgg
     2161 tgcagggtca ccgccataga actgtccctc ccgagtaccc cgaaatgata cgccaccccc
     2221 ttgagaaatc gtcgcgccgg cggggcgcgg ctcaggaccg gcgggcgccg cccgcgaaga
     2281 tctcgtgccg cgcggcgtcc gagatcgcga cgacggccgg gttccggagg cgccgctcga
     2341 cggacaccgc gtagaagcgc tccttcacgt ccggcgccgc gccgacgacc ttcaccccgt
     2401 actgccggca gacctcctcc tccacggcgc tcggcgccgt gaagacgccg agcccgtccg
     2461 cgccgaacac cttgaggagc gcgctgtcct ggaactccgc gacgacgcgc ggacggatgc
     2521 cgcggcggtc gaaccagtgg tcgagagtcc gccgcaggac cgagctctcg agcggcagga
     2581 gcacgggggc gccgtggagg gaccgcggaa agccgcgccg gacggccctc gcgagggcgg
     2641 gagccgcgaa gaagctcacg cccgtctcgc cgagcaggtg gtggaaggcg cggacgcggc
     2701 tgccggaggg cacgggcgcg tccgcgatga cgacgtccag cgtgtggagc gcaaggtccg
     2761 cgaggagcct ctcgtacgag tcctcgcggc atacgatcct caccggctcc ggcagcgcga
     2821 gcgcgggctc gaggagccgc cgcacgacga ccttcgggac cacgtcgacg acgcccacct
     2881 cgagacggag cggccggccc gtcgcccggt cgcgcaccgt gtcgaccatc tcgcgcccga
     2941 gcgtgaagat ctcgtcggcg tagccgaaga cgatccggcc cacctccgtg agggcgagct
     3001 tccgcccctc cttcgcgaag agcttctccc cgagccggtc ctcgagcgcg tggatctgcg
     3061 cgctgagggt cgggtgcgag aggcgcagga ccttcccggc ggggacgaga ccgccctccc
     3121 gggcgacgag ccagaaatag aggaggtggt ggtagttgat ccattcgagg cgcggcccaa
     3181 tcatgtcggt cattccgaca gtttacgttg agatttcgta atggactaca tgactcggag
     3241 gccctaaaca agggggcgtc gcgccggcgt ggcgcgacga agggaggccg gacatgaaga
     3301 tcgacgttcg tttccggggg atccaggttt cggaggcgct ccgcgagtac gccgtccgcc
     3361 gcgtccgttt ccagctcagc cggttcgacg gcgacgtggg ctcggtcgtc gtccggatcg
     3421 gcgacgtcaa cggcccgcgc ggcggagtcg acaagcgctg tctcgtcgag gcgcgcggcc
     3481 ccgcgttcgg cccggtcgcc gtcgaggacg cgagcacgga cgcctacacg gccgtcggat
     3541 cggccgtcgg gcgcgccgcc cgcgcggtgg ggcgcgagat cgagcgggcg cgggtcggtc
     3601 agcgcgaaga tggtcctgtg ggcgagc
//
LOCUS       DAOMFY010000344         3600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10203_length_3600_cov_9.570099, whole genome shotgun sequence.
ACCESSION   DAOMFY010000344 DAOMFY010000000
VERSION     DAOMFY010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10203_length_3600_cov_9.570099"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..346)
                     /gene="rpmH"
                     /locus_tag="PLB01_18235"
     CDS             complement(203..346)
                     /gene="rpmH"
                     /locus_tag="PLB01_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQQ79294.1"
                     /translation="MAKTKRTFQPNNRHRKRTHGFLVRMRTKTGRAVLARRRAKGRKT
                     LSA"
     gene            490..1803
                     /gene="dnaA"
                     /locus_tag="PLB01_18240"
     CDS             490..1803
                     /gene="dnaA"
                     /locus_tag="PLB01_18240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQQ79295.1"
                     /translation="MEKANSWAKILEHLKTRVDEREFDSWFRDSRQKSETADAIFVQV
                     RTPLFVDYIPEAYAQQIAEAARLAGVGTREIRFGADGDYRSAVNPQKVPAEPRRRAGG
                     LNPAYTFENFVTGSSNRLAHAAALRVADQTSRQYNPLLVCGGTGLGKTHLMQAIGHRR
                     LSRHPGEKVIYLTSENFVNEVVLGLRFNKMEAVRQRFRAADILLLDDVQFVVGKEASE
                     NELFHTFNALYDEGNQIVFSSDVPPREIPGLTERLKSRFEGGLIVDIQLPDFETKVAI
                     LRQKADAHEVALDDDVSFFLASKIKSSIRELEGYFNRVVAYASLKGEPVSLDLARESL
                     KDVFRAEESVASPAEIVKGIAAHYGLKPNEMRARSKRGPIVFPRQVAMYVLKEATHLS
                     LPEIGRLFSDKHHTTALHAVRKIAAMREEDPEFDRLVAGFLAQYR"
     gene            2049..3173
                     /gene="dnaN"
                     /locus_tag="PLB01_18245"
     CDS             2049..3173
                     /gene="dnaN"
                     /locus_tag="PLB01_18245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQQ79296.1"
                     /translation="MELTIPASVLARELSLIQGVVEKRTTHQILTNVLLEARNGVLAL
                     TATDLDTTLVSEIPTTAQKSAGAVTAPARRLYDVVRELPPNAELRLKVLENNHVSLEC
                     KEPKLRIRLNGLPAGDYPTRPEPAGGPSLTLPFGALRRMVDKVRFAVSTEEMRLQLQG
                     ALLKAKNGVVEMVATDGHRLALVEVDGSKADSGFEPVLVSKKILDELGKLDAADDAAV
                     TIVRGTNHIGFVVGARKLFSKLDERRFPDYEKVISKENDKKAVVPREELLGAVRRIAL
                     LSGDRTKAVSLAFDKGSLVVSSESQEVGDGSQEIPATYDAAPVTLRLNARYLEQFLEA
                     AETKDVHLFVKDEQAQCQGRPGEPLGGIRSYLYVVMPMRM"
     gene            3182..>3600
                     /locus_tag="PLB01_18250"
     CDS             3182..>3600
                     /locus_tag="PLB01_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006985615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQQ79297.1"
                     /translation="MEILSLTSDAFRNLASTRLEFHPRLNLVVGDNGQGKTNLLEALA
                     LVSGRASFRTRDLAEVREHLSPRTVVSARVREAGDPRTDATLGLVLAAGVREHYRDGK
                     RVTRLAAARALPAVFLTSRDLARLSGPAADRRRALDRA"
BASE COUNT          586 a         1237 c         1176 g          601 t
ORIGIN      
        1 ggtcgcgcga agacgacgac gagccggccg tgatggcggc ggcccgcgtc gtacgtcgcc
       61 gtgaagtccc ggcgcgcggc gatcctcagg cggcgcggaa acgcctccgc gcgcgggcgg
      121 cgcggggcgg gcggggcgct tgggttcatg gaccggctct tgggccgggg gagcccggcg
      181 gcggcccagg cccctcctcc gctcaggcgg agagggtctt gcggcccttg gcccggcggc
      241 gggcgaggac ggcgcggccc gtcttggttc tcatccggac gaggaacccg tgcgtcctct
      301 tccggtggcg gttgttgggc tggaatgtgc gcttcgtttt ggccatggtt ggcgggccct
      361 ccggcgggga gggggattct ccgggtccgg gccccgcctg tcaacccgat gcgccttgga
      421 cggccggaga gcgggtggta ggctctcgcc ccccgacgcc gaatcgattt gcacagcggt
      481 ggacatcctg tggaaaaagc caactcctgg gccaagattc tcgaacacct gaagacccgc
      541 gtcgacgagc gggagttcga ctcctggttt cgcgattccc ggcagaagtc cgagaccgcc
      601 gacgcgatct tcgtccaggt gcggacgccc ctcttcgtcg attacatccc tgaggcttac
      661 gcccagcaga tcgccgaagc cgcccgtctc gcaggcgtcg ggacccgcga gatccggttc
      721 ggcgccgacg gggactaccg ctccgcggtg aacccgcaga aggtcccggc cgagccgcgg
      781 cgccgggccg gcggtctgaa ccccgcctac acgttcgaga atttcgtgac gggttcgtcg
      841 aaccggctcg cccacgcggc cgcgctgcgg gtcgccgacc agacctcgcg ccagtacaac
      901 ccgctcctcg tctgcggcgg cacgggtctc ggcaagacgc acctcatgca ggccatcggg
      961 caccggcgcc tctcgcgcca cccgggcgag aaggtcatct acctgacgtc cgagaatttc
     1021 gtgaacgagg tcgtcctcgg cctgaggttc aacaagatgg aggccgttcg ccagcggttc
     1081 cgcgccgccg acatcctcct cctcgacgac gtccagttcg tcgtcggcaa ggaagcctcc
     1141 gagaacgagc tcttccacac gttcaacgcg ctttacgacg agggcaacca gatcgtcttc
     1201 tcctcggacg tgccgccgcg cgagatcccc ggcctcacgg agcgtctcaa gagccgtttc
     1261 gagggcgggc tcatcgtgga catccagctg cccgatttcg agacgaaggt cgcgatcctc
     1321 cggcagaaag cggatgcgca cgaggtcgcg ctggacgacg acgtctcgtt cttcctcgcc
     1381 tcgaagatca agtcgtcgat ccgcgagctg gagggctact tcaaccgcgt ggtcgcgtac
     1441 gcgtcgctca agggcgagcc cgtctcgctc gacctcgcac gcgagtcgct gaaggacgtc
     1501 ttccgcgccg aggagtccgt tgcgtctccc gccgagatcg tcaagggcat cgcggcgcac
     1561 tacgggctga agccgaacga gatgcgtgcg cggtccaaac gcgggccgat cgtctttccc
     1621 cgccaggtcg cgatgtacgt cctgaaggag gcgacgcacc tctcccttcc ggagatcggc
     1681 cgtctcttct ccgacaagca ccacacgacg gcactccacg cggtgcgcaa gatcgcggcg
     1741 atgcgcgagg aggatcccga gttcgaccgt ctcgtcgcgg gtttcctggc gcagtaccgc
     1801 tgacttttcc acgctttttt ccacccgttt tcctccccgc cgagcggcct cttggataac
     1861 ccgttgagcg cgtttttgct gccccgtgaa tacacaatcg attggcaggg gatacacagg
     1921 gtattatgtt actcaagtca tttaaaataa atgatttacg tacatctaca ctttttgaca
     1981 cttcctacga cttctacttc ttttgggggg ttctggatga tacgattcag attccttaag
     2041 gagtcgtcgt ggagctgaca attccggcct cggttctcgc ccgcgaactc agcctgattc
     2101 agggcgtcgt ggagaagaga accacgcacc agatcctcac gaacgtgctt ctcgaagcgc
     2161 gaaacggcgt cctcgccctc acggcgacag atctggacac gacgctcgtg tccgagatcc
     2221 cgacgacggc gcagaagagc gccggggccg tcacggcgcc ggcccgccgg ctttacgacg
     2281 tcgtgcgcga gctgccgccg aacgccgaac tcaggctcaa ggtgctcgag aacaatcacg
     2341 tctccctcga gtgcaaggag ccgaagctgc ggatccggct caacggcctg ccggccgggg
     2401 actaccccac gcgcccggag cctgccgggg gaccgtcgct cacgctcccg ttcggcgcgt
     2461 tgcgccgcat ggtcgacaag gtccgtttcg cggtctcgac cgaggagatg cgccttcagc
     2521 tgcagggggc gctcctgaag gcgaaaaacg gcgtcgtcga gatggtcgcg acggacggac
     2581 accggctggc cctcgtcgag gtggacgggt cgaaagccga ttcgggattc gagcccgtgc
     2641 tcgtttccaa gaagatcctg gacgagctcg gaaaactcga cgcggccgac gatgcggccg
     2701 tgacgatcgt gcgcggcacc aaccacatcg ggttcgtcgt cggcgcgcgc aagctcttct
     2761 cgaagctcga cgaacggcgc tttcccgact acgaaaaggt catcagcaag gagaacgaca
     2821 agaaggccgt cgttccgcgc gaggagctcc tgggcgcggt ccgccgcatc gcgctgctct
     2881 ccggcgaccg gacgaaggcc gtgagcctcg cgttcgacaa gggctccctc gtcgtctcgt
     2941 ccgagagcca ggaggtcggc gacggctcgc aggagatccc cgcgacgtac gacgccgccc
     3001 ccgtgacgct tcgcctcaac gcacgctacc tcgagcagtt tctcgaggcg gccgagacca
     3061 aggacgtcca cctgttcgtc aaggacgagc aggcccagtg ccagggtcgg cccggagagc
     3121 ccctcggcgg catccggagc tatctctacg tcgtcatgcc gatgcggatg taaaaggcct
     3181 agtggagatt ctgtccctca cttcggacgc gttccggaac ctcgccagca cccggctcga
     3241 gttccacccg aggctcaacc tcgtggtggg agacaacggg cagggcaaga cgaacctcct
     3301 cgaggcgctc gcgctggttt ccggacgggc gtcgttccgc acccgcgacc tggcggaagt
     3361 gcgcgagcac ctctccccgc gcacggtcgt gtccgcgcgc gtacgcgagg cgggcgatcc
     3421 gcgcaccgac gcgacgctcg gcctcgtcct cgcggccggg gtccgcgaac actaccggga
     3481 cggaaagcgc gtgacgcggc tcgcagccgc ccgcgcgctg cccgcggtct ttctcacgtc
     3541 gcgcgacctc gcgcgccttt cggggccggc cgccgaccgg cgccgcgcgc tcgaccgggc
//
LOCUS       DAOMFY010000345         3581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10301_length_3581_cov_6.289563, whole genome shotgun sequence.
ACCESSION   DAOMFY010000345 DAOMFY010000000
VERSION     DAOMFY010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10301_length_3581_cov_6.289563"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLB01_18255"
     CDS             complement(<1..397)
                     /locus_tag="PLB01_18255"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQQ79298.1"
                     /translation="MSLPHVLLGLLSATPASGWDLKRRLGEDPARGWDADLSQIYPAL
                     RRLLRGGFVSLKRRRSPQGPPRREYRVTPSGRRELKEWLAEPYALPRPHDATLARLAL
                     LERSLPPDRVRRLQEYRGLLADELKRAAAG"
     gene            989..1837
                     /locus_tag="PLB01_18260"
     CDS             989..1837
                     /locus_tag="PLB01_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ79299.1"
                     /translation="MTDALVLAHSPDSDDAFMFYGLRANAVDTEGVTYTHTLADIETL
                     NRRALSGELAITAVSFHAYAYLADRYVLLPHGASFGDGYGPCVVARREGGPRTLADLS
                     GKRVAVPGTLTSAAMCLSLYAKDATPVVMAFDKVGDAVNAGEVDAGVVIHEGQLTWKD
                     EGFHLVADFGVVWKEETGLPLPLGGNAVRRDLGPELIARVSRALGRSIEYALAHREDA
                     LTDAMRFARGLPREKADRFVGMYVNDWTRSYGPKGREAVRLFLRRGFEAGILPKLVEP
                     EWVAEG"
     gene            1842..2342
                     /locus_tag="PLB01_18265"
     CDS             1842..2342
                     /locus_tag="PLB01_18265"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HQQ79300.1"
                     /translation="MTKSARRLAAWFLFLAALWCGMLAFFGAFGARVVLDASPSRHAA
                     GAVNKVLLDVLDAASLIFVLVLGLLLVFWNRARPWTRGARGYAVRFLAIAAVAAAASL
                     FVVTPEMMERRARMGPIDLVSKEDPLRKAWGRLHGVSSITLLVRILASAGVFAAGFGQ
                     LPRGDA"
     gene            complement(2347..3225)
                     /locus_tag="PLB01_18270"
     CDS             complement(2347..3225)
                     /locus_tag="PLB01_18270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ79301.1"
                     /translation="MRSLAEILLSPVGLAPVTASAEAYRTAVAAAAGDAGVVETLVHD
                     GDPAEPARLVTLFASPGRPGTRPTLLFLHGKGGGGAEWTKDAVRALRLGFNVLVPELR
                     GHAPSTGARITCGIRETNDLLLLLDLARARFGVEAARLGIDGCSYGSLLAVQLAAARA
                     PAALWLQSPFGDLTAMAVHYLHRATGLPRVLLEGPVRLLVRRIERTEKLDLSQADPVA
                     AARRVTCPTTVVHGEADALVPIRFSPRLYEALAGPKSFWRVPACGHCHHADEPQAVVP
                     DAYERRWTEFFLQNVP"
     gene            complement(3242..>3581)
                     /locus_tag="PLB01_18275"
     CDS             complement(3242..>3581)
                     /locus_tag="PLB01_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79302.1"
                     /translation="AAGRPADAKKAWASALELAAGAMDAGLSTRASRAALALKDSPEA
                     VKHARRAVELTQGEDAPAQAALAQALRASGDAAGSSAAWKRAAELDPQNAEYRAGAGI
                     DAKRKGAKAS"
BASE COUNT          491 a         1201 c         1331 g          558 t
ORIGIN      
        1 agccggccgc ggcgcgcttg agctcgtcgg cgaggaggcc gcggtactcc tggaggcggc
       61 gcacgcggtc gggcgggagg ctgcgctcga ggagggcgag cctcgcgagc gtcgcgtcgt
      121 gcgggcgggg gagcgcataa ggctcggcga gccattcctt cagctcgcgg cgacccgagg
      181 gcgtcacgcg gtactcccgc cgcggggggc cctgcggcga acggcgtcgc ttgagcgaga
      241 cgaaccctcc gcgcaggaga cggcgcagcg cgggatagat ctgggagaga tccgcgtccc
      301 atccgcgcgc gggatcctcg ccgaggcgcc gcttgaggtc ccacccgctg gccggagtcg
      361 ccgagaggag gccgaggagg acgtgcggga gcgacatgcc ggaagtatat gagacttcac
      421 ataccgccgt gcaaaacacg aggattacgc tgtggaaatc tcggggtccg gagtgcgcca
      481 ggatgacgtc cggacaaaaa aataaggggg ctggtgtagc ccccgaatcc cggaaatacc
      541 ttggattgga atggtttgaa gtaacgaagt tacttcttgc ccttcttcgc cttcttggtg
      601 ctcttcttcg cagctttctt cttggccggc atggtttctg tcacctcctt actggcaggt
      661 ttcgttgaca gtgccggaag ctgcgtgcta ggttgtccgc ccctcgaagg acggggacta
      721 gcgcttccgg attctgtcga ctgccctgag tcacttaaga gcgtcgatcg atttcaaaag
      781 acctggttcc cgtgagacgg catcgtgaat cgatcacggc gccgtctctt tttttctgct
      841 gagtgaattg tatagaggct cgacggcgcg aatcaagaat ttttttcttg acgggcgaag
      901 tttttttcac ccgaccgttc gtcgcctcgc ggggggtctc gcgcgcatcc gggctaggat
      961 cgcgcgcgga gagaatcgga gccctcacat gacagacgca ctcgtgctcg ctcactcgcc
     1021 cgactccgac gacgcgttca tgttctacgg actgcgcgcg aacgccgtgg atacggaggg
     1081 cgtcacctac acgcacacgc tcgcggacat cgagacgctc aaccgccgcg cgctctcggg
     1141 cgagctcgcg atcacggccg tgtcgttcca cgcgtacgcg tatctcgccg accgctacgt
     1201 gctcctgccg cacggcgcgt cgttcggcga cggctacggg ccgtgcgtcg tggcgcgccg
     1261 cgaaggcggg ccacgcacgc tcgcggacct ttcgggcaag cgggtggccg ttccggggac
     1321 gctcacgtcg gcggcgatgt gcctctcgct ctacgcgaag gacgcgacgc cggtcgtcat
     1381 ggccttcgac aaggtcggcg acgccgtcaa cgccggcgag gtggacgcgg gggtcgtgat
     1441 ccacgagggc cagctcacgt ggaaggacga gggcttccat ctcgtcgccg acttcggcgt
     1501 cgtctggaag gaggagacgg gcctcccgct tccgctcggc ggcaacgccg tccggcgcga
     1561 cctcggtccg gagctcatcg cgcgggtctc gcgcgcgctc ggccgatcca tcgagtacgc
     1621 ccttgcgcac cgcgaggacg cgctcacgga cgcgatgcgc ttcgcgcgcg gcctcccgcg
     1681 cgagaaggcc gaccgcttcg tcgggatgta cgtgaacgac tggacgcgtt cctacggacc
     1741 gaagggccgg gaagccgtgc gtctcttcct gaggcggggg ttcgaggccg gcatcctgcc
     1801 gaagctcgtc gagccggagt gggtggcgga gggttagggg cgtgacgaag agcgcgcgga
     1861 ggctcgcggc ctggtttctc tttctcgccg ccctctggtg cgggatgctc gcgttcttcg
     1921 gcgcgttcgg cgcccgggtc gtcctcgatg cgtcgccgag ccgccacgcg gccggcgccg
     1981 tgaacaaggt ccttctggac gtcctcgacg ccgcgtcgct catcttcgtg ctcgtcctcg
     2041 ggctccttct cgtcttctgg aaccgcgcgc ggccctggac ccgcggcgcc cggggctacg
     2101 ccgtccgctt tctcgcgatc gcggcggtcg cggccgcggc atcgctgttc gtcgtgacgc
     2161 ccgagatgat ggagcggcgc gcccgcatgg gtcccatcga cctcgtctcg aaggaagacc
     2221 ccctgcgcaa ggcgtggggc cggctgcacg gcgtgtcgtc gatcacgctc ctcgtgcgga
     2281 tcctcgcctc ggccggcgtc ttcgcggcgg gcttcgggca gcttccgagg ggcgacgcct
     2341 gacgccctag gggacgttct ggaggaagaa ctccgtccag cggcgctcgt acgcgtccgg
     2401 gacgacggcc tgcggctcgt ccgcgtgatg gcagtgcccg cacgcgggga cgcgccagaa
     2461 cgacttcggg cccgcgagcg cctcgtagag gcgcggcgag aagcggatcg gcacgagggc
     2521 gtccgcctcg ccgtggacga ccgtcgtcgg gcacgtgacg cgccgggcgg ccgcgacggg
     2581 gtcggcctgc gagaggtcga gcttctcggt cctctcgatc cggcggacga ggagacggac
     2641 cggcccctcg aggaggacgc gggggaggcc cgtcgcgcgg tggaggtaat ggaccgccat
     2701 cgcggtgagg tcgccgaacg gcgactggag ccagagcgcc gcgggcgccc gggcggccgc
     2761 gagctggacg gcgaggagag agccgtacga gcagccgtcg atcccgaggc gcgcggcctc
     2821 gaccccgaag cgggcgcggg cgaggtcgag gaggagcagg aggtcgttcg tctcgcggat
     2881 gccgcacgtg atccgcgccc ccgtcgacgg cgcgtggccg cgcagctcgg gaacgaggac
     2941 gttgaagccg agcctcagcg cccggaccgc gtccttcgtc cactcggctc cgcccccgcc
     3001 cttgccgtgc aggaagagaa gcgtcggccg cgtcccgggc cggcccggcg aggcgaagag
     3061 cgtcacgagc cgggcgggct cggccgggtc gccgtcgtgg acgagcgtct cgacgacgcc
     3121 ggcgtcgccc gcggcggccg caacggccgt gcggtaagcc tccgccgacg cggtgacggg
     3181 cgcgaggccg acgggcgaaa ggaggatctc cgcgagcgag cgcatgcgcg gaggcgaagg
     3241 ctcaggaggc cttcgcgcct ttcctcttcg cgtcgatccc ggcgcccgcg cggtactcgg
     3301 cgttctgcgg gtcgagctcg gcggcccgct tccaggccgc ggaggatccg gcggcgtcgc
     3361 ccgaggcgcg aagcgcctgc gcgagagcgg cctgagcggg ggcgtcctct ccctgcgtca
     3421 gctccacggc gcggcgggcg tgcttgacgg cctccgggga gtccttgagg gcgagcgcgg
     3481 cgcgcgaagc gcgcgtcgaa aggccggcgt ccatcgcccc ggcggcgagc tcgagggccg
     3541 aggcccaggc cttcttcgcg tcggcgggcc ggcccgcggc g
//
LOCUS       DAOMFY010000346         3577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10321_length_3577_cov_6.442362, whole genome shotgun sequence.
ACCESSION   DAOMFY010000346 DAOMFY010000000
VERSION     DAOMFY010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10321_length_3577_cov_6.442362"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLB01_18280"
     CDS             <1..227
                     /locus_tag="PLB01_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79303.1"
                     /translation="ARAAEEVAARLVVRLAGREIAHVVPVLGALAGAGLNYAAVRAVG
                     RAALARVERLHGPPEIPGRGPVLDVPGRIA"
     gene            328..2214
                     /locus_tag="PLB01_18285"
     CDS             328..2214
                     /locus_tag="PLB01_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ79304.1"
                     /translation="MRKVVYRSIRGAANAAWNVLQAVNRRVPARAFQPSWSPEPLPKS
                     HERTKPPLGFPRRTDSLCPTCTREVRDAIVGGTRELRELVDGRPGEIPADIVQRGNEI
                     WMVKTCPKHGTFEDLMASDARFFARLEENFFGRDVKIAKDKLHDHGSASMRYGRGAVL
                     TVDLTNRCNMMCNPCFMDANQVGYVHELGWDDIKEILDNAVSIKPQRQMSVQFSGGEP
                     TISPFFIEATRYAREIGYFSVQCATNGIRFAQEPEFAKAAYDAGLRLAYLQFDGVGNE
                     PNAHRGVGNLYDVKLRALDNLHAAGIDVTLVTTIVNGINNDQVGPTVKFAIENIDKIN
                     ALSFQPVSFTGRDEEIDDDTRKSQRYTLAHLAHDVKDQAGIGEPMRDWFPLSASGPFS
                     DLKDLLGGLEAQWGSLKCGCHPNCGIATMMLVRPDTGQAVPLTQIVDADRLLRDLTVV
                     TDAARGRVLTVLQTALALARNIRPEGVPHNLGVWETLKVMDGHTGKGMGIAKQGRYPW
                     RVLLVAGMWFQDLFNYDFRRTEMCIIPYGTQLGEISFCAYNTGAGWRKIVEEMHQSAT
                     LSEWYKEKGRHPVYAHGKRVPLPAFNPAARPSTLPVLEPAAARAGELPLTPMLPSFGA
                     ASGK"
     gene            complement(2224..3009)
                     /locus_tag="PLB01_18290"
     CDS             complement(2224..3009)
                     /locus_tag="PLB01_18290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ79305.1"
                     /translation="MHLVALHGYPLDRRMWDPLAERAADGRLGPITSVFAPDLRGRGR
                     SPHPAAATHAMSLLADDVARDLETALPAGAPFLLAGLSMGGYVCFELVKRHGARFRGR
                     LRGLALFDTKASADDAAGRAGRRAAIEAIRKDGIEAALAAMLPKLLARGSKGTPAEDL
                     VKRMVVATPPETAMADLAGLAVRDEGFEVLSAWDRPLLVAVGEEDAIAPPSDAEAMTA
                     VAARAPWVRLLTVPGAGHMVPLERPDEAAAGLVSLAEAALRVP"
     gene            complement(3020..>3577)
                     /locus_tag="PLB01_18295"
     CDS             complement(3020..>3577)
                     /locus_tag="PLB01_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79306.1"
                     /translation="GLGALAAARLALVLPVVFRRLRRRGGGPLRLLAAAGGAAAFAVF
                     IGVAALGAGPLLLDRVGGPVFSWDDSATRAAAAHVRSRVPAGGALLAHGTPQNLYPLT
                     GTRAPGGFYVNPSFWYCLKRDRGDQRLVDALAASPGLPILFREPIADAERIRETAVYA
                     FVKERTRPAGKAGDAEWRVVSAPDR"
BASE COUNT          552 a         1291 c         1275 g          459 t
ORIGIN      
        1 cggcgcgcgc ggccgaagag gtcgccgcgc gcctcgtcgt ccgcctcgcg ggacgcgaaa
       61 tcgcgcacgt cgtgccggtt ctcggggcgc tcgcgggcgc gggcctcaac tacgcggccg
      121 tgcgtgccgt cgggcgggcg gctctcgcgc gcgtcgagcg cctgcacggc ccgcccgaga
      181 ttccgggccg agggccggtg ctcgacgtgc ccggacgcat cgcgtaagga tcgtttgccc
      241 ggtgcatgct aaattcccgg cttcacgggc gttccgttga gaatgattct cctcggggac
      301 ggagccgtgt atctttggag gatcgaaatg cggaaggtgg tctaccggtc cattcgcgga
      361 gcggccaacg ccgcctggaa cgtgctccag gccgtgaacc gccgcgtgcc cgcgcgggcc
      421 ttccagccct cctggtcgcc ggagccgctc ccgaagagcc acgaacgcac caagccgcct
      481 ctcgggttcc cacgccgcac ggattccctc tgcccgacct gcacccgcga ggtccgcgac
      541 gcgatcgtgg gcggcacgcg tgagctccgc gaactcgtcg acgggcgccc cggcgagatc
      601 cccgcggaca tcgtccagcg gggcaacgag atctggatgg tcaagacgtg cccgaagcac
      661 ggcacgttcg aggacctcat ggcgagcgac gcgcgcttct tcgcgcgcct cgaggagaac
      721 ttcttcggcc gcgacgtgaa gatcgcgaag gacaagctcc acgaccacgg ctccgcttcg
      781 atgcgctacg gccgcggcgc ggtcctcacc gtggacctca cgaaccgctg caacatgatg
      841 tgcaacccgt gcttcatgga cgccaaccag gtcggctacg tccacgagct cgggtgggac
      901 gacatcaagg agatcctgga caacgccgtc tcgatcaagc cgcagcgcca gatgtccgtg
      961 cagttctcgg gcggcgagcc gacgatctcg ccgttcttca tcgaggcgac gcgctacgcc
     1021 cgcgagatcg gctacttctc ggtccagtgc gcgacgaacg gcatccgctt cgcgcaggag
     1081 ccggaattcg cgaaggccgc gtacgacgcg ggcctgcgcc tcgcgtacct ccagttcgac
     1141 ggcgtcggaa acgagccgaa cgcgcaccgc ggcgtcggga acctctacga cgtcaagctc
     1201 cgcgcgctcg acaacctcca cgccgccggg atcgacgtca cgctcgtcac gacgatcgtg
     1261 aacggcatca acaacgacca ggtcggcccg accgtgaagt tcgcgatcga gaacatcgac
     1321 aagatcaacg cgctctcctt ccagcccgtg tccttcacgg gacgcgacga ggagatcgac
     1381 gacgacacgc ggaagagcca gcgctacacg ctcgcgcacc tcgcccacga cgtgaaggac
     1441 caggcgggca tcggcgagcc catgcgcgac tggttcccgc tgtcggcgtc cggccccttc
     1501 tccgacctga aggaccttct cggcggcctc gaagcgcagt ggggctcgct caagtgcggc
     1561 tgccacccga actgcgggat cgcgacgatg atgctcgtgc gcccggacac gggacaggcc
     1621 gtgcccttga cccagatcgt cgacgcggac cgcctcctcc gcgacctgac cgtcgtcacg
     1681 gacgccgcgc gcgggagggt cctgacggtt ctccagacgg cgctcgcgct cgcgcgcaac
     1741 atccggcccg agggcgtccc gcacaacctc ggcgtctggg agacgctcaa ggtcatggac
     1801 ggccacacgg gcaaggggat ggggatcgcg aagcagggcc gctacccctg gcgcgtcctc
     1861 ctcgtggccg ggatgtggtt ccaggacctc ttcaactacg acttccggcg cacggagatg
     1921 tgcatcatcc cgtacggcac gcagctcggc gagatctcct tctgcgccta caacaccggc
     1981 gcgggctggc ggaaaatcgt cgaggaaatg caccagagcg cgacgctctc cgagtggtac
     2041 aaggaaaaag gccgccaccc cgtgtacgcg cacggcaagc gcgtgccgct gccggccttc
     2101 aacccggccg cccggccctc gaccctcccg gtcctcgaac ccgccgcggc gcgggcgggc
     2161 gagctcccgc tcacgcccat gctcccgagc ttcggcgccg cttccggaaa atagagcccg
     2221 gcctcaggga acgcggagag cggcctcggc gagcgacacg aggcccgccg cggcctcgtc
     2281 cggccgttcg agcgggacca tgtggccggc gccgggcacg gtcaggagcc tcacccacgg
     2341 cgcgcgtgcc gcgacggctg tcatggcctc ggcgtcggag ggcggggcga tcgcgtcctc
     2401 ctcgccgacg gcgacgagga gcggccggtc ccacgcggag aggacctcga agccctcgtc
     2461 gcgcacggcg aggccggcga ggtcggccat ggcggtttcc ggcggcgtgg cgacgaccat
     2521 ccgcttcacg aggtcctcgg ccggagtccc cttcgaaccg cgggcgagga gcttcgggag
     2581 catcgcggcg agcgccgcct cgatgccgtc cttgcggatg gcctcgatgg cggccctccg
     2641 gccggcgcgt cctgccgcgt cgtcggcgga ggccttcgtg tcgaagaggg cgaggccccg
     2701 gaggcgcccg cgaaagcgcg cgccgtgccg cttgacgagc tcgaagcaga cgtagccccc
     2761 catcgagagg cccgcgagga ggaacggcgc gccggcgggg agcgccgttt cgaggtcgcg
     2821 cgcgacgtcg tccgcgagga gcgacatcgc gtgggtcgcg gcggcgggat gcggcgaccg
     2881 cccgcgtccg cgcaggtcgg gggcgaagac ggacgtgatc ggtccgagcc ggccgtccgc
     2941 ggcccgctcg gcgagcgggt cccacatgcg ccggtcgagg ggatagccgt gaagcgcgac
     3001 gagatgcatc ggcgccatct tagcggtcgg gcgcggaaac gacgcgccac tccgcgtcgc
     3061 cggcctttcc ggccggccgc gtcctctcct tcacgaaggc gtagacggcc gtctcgcgga
     3121 tccgctccgc gtcggcgatc ggctcccgga agaggattgg caggccgggg ctcgcggcga
     3181 gggcgtccac gagccgctgg tcgccgcggt cgcgcttcag gcagtaccag aaggacgggt
     3241 tcacgtagaa gccgccgggc gcgcgcgtgc cggtgagcgg atagaggttc tgcggcgtgc
     3301 cgtgggcgag gagcgcgccc ccggcaggaa cgcgcgacct cacgtgcgcc gcggcggccc
     3361 gggtcgccga gtcgtcccag gagaagacgg gtccgccgac ccggtcgagg agcaacgggc
     3421 ccgcgcccag agcggcgacc ccgatgaaga cggcgaaggc ggcggcgccg ccggcggccg
     3481 cgagaaggcg cagcggcccg ccgccgcgtc tccggagccg gcgaaacacg acgggcagaa
     3541 cgagggcgag ccgcgcggcc gcgagcgcgc cgagccc
//
LOCUS       DAOMFY010000347         3574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10340_length_3574_cov_5.047457, whole genome shotgun sequence.
ACCESSION   DAOMFY010000347 DAOMFY010000000
VERSION     DAOMFY010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10340_length_3574_cov_5.047457"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLB01_18300"
     CDS             <1..745
                     /locus_tag="PLB01_18300"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79307.1"
                     /translation="RRPARSLGGVLLRAETATVLRDASTPAQLGSLAAVFLLDLLNVR
                     LLPASDPAARDLVAGLQTGLSLFLVSALSLRFAYPAVSTDARAAAVLRSLPLDPRRHL
                     LARWAARALPAVLASLVLTGASLAVLKPAAATTTAALAVTLSGGLAIPALHTGLGGLF
                     PRYDAPNPVAVALGPGGLFALVLSTGLALFATVAASQELRTLLGALTHVRVGTWTPLG
                     GFLCAAAVAAAVPMALAGRSLERQDLSIG"
     gene            complement(777..1688)
                     /locus_tag="PLB01_18305"
     CDS             complement(777..1688)
                     /locus_tag="PLB01_18305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HQQ79308.1"
                     /translation="MKVFVGQPLVEPSTQALLAGHAVVTGAGSLPRAEWPLLADADAL
                     LPTPRERITEEVLAAAPRVRIVASHSVGVDHVDLAACRARGVLVTNTPDVLTDATADL
                     AFALILAVARRMHEGEALARSGTWDGWKPQELLGVSLAGKTLGIYGMGRIGRAVALRA
                     EAFGMDVISFEKDGTEEEFEEFLSEVDVLSIHAPLTEETRGRLGEKEFLRMKKGTILV
                     NTARGPIVDEKALAAALHSKHLFGAGLDVFQREPQIEATLLGLPNVVLLPHLGSATTE
                     TRLAMARLACTEIVRFFAGEPPLNPVG"
     gene            complement(1685..1858)
                     /locus_tag="PLB01_18310"
     CDS             complement(1685..1858)
                     /locus_tag="PLB01_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79309.1"
                     /translation="MRFAIGRALQLVGIVLVGVGLAVGLFRDDLRYEERMFFAGGGVF
                     VAGWLLVRPYKSS"
     gene            complement(1855..>3574)
                     /locus_tag="PLB01_18315"
     CDS             complement(1855..>3574)
                     /locus_tag="PLB01_18315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQQ79310.1"
                     /translation="FAFGLGKGATDALLAALAARDGATSTRAASALAMFRKPEARTVS
                     ALVTVFEENSLAPELRRAAVYSLSRKPQPEAAPALRAVLRMKGEGAATPSSDEVAWAA
                     RGLGILGDEESAADLVTLARSPGVSVAVQALGALYSLSKKNLFSESSDLTRTTRSAAL
                     LRANDSLPGVALAALRLLGALPDSPESRAALEENLLRKGWRGQSALVALTRLDAARAP
                     DKASARLEKQILGGTLEQRLGAAEALEFLGDDEKTGEGKRGIVSSDPLADALLSDPSA
                     RVRAAAVSSLSKKKTAKRNAWLLAALIDRDPAVREVALEEAAPLLEKGSVELRRAWKS
                     AFDGAFSSNEPDFTVGALDAAAARGEAGRALVAAHANDADAVTRDKARRVLVEKYGAE
                     AASFRPIPVKTQLTGADYVRLARAANESSFSATVETARGTAVLDLDAEAAPITVESFR
                     ALAAKRFFDGIVIHRVVPDFVVQTGDPRGDGSGGPGYAIRDEINPLRYARGAVGMALS
                     GPDTGGSQWFVTLAPQIHLDGGYTVFGGVRSGMDLFDRTEQDDALVSVRVTERPRPAR
                     PPGAVE"
BASE COUNT          506 a         1324 c         1201 g          543 t
ORIGIN      
        1 ccgccgcccc gcacgttcgc tcgggggcgt cctcctgcgc gccgagacgg ccaccgttct
       61 gcgcgacgcg tccacgcccg cgcagctcgg atctctggcg gcggtcttcc ttctcgacct
      121 cctgaacgtg cgcctcctcc ccgcgtcgga tcccgcggcc cgggatctcg tcgccgggct
      181 gcagacgggc ctctccctct tcctcgtgtc ggccctctcg ctgcgtttcg cctaccccgc
      241 ggtctcgacg gacgcgcgcg cggctgcggt gctccggtcg ctgccgctcg acccccggcg
      301 ccatctcctc gcccgctggg cggcccgcgc acttcccgcc gtcctcgcct cgctcgtcct
      361 gacgggtgcg agcctcgccg tcctcaagcc cgccgccgcc acgacgaccg ccgcgctcgc
      421 ggtcacgctc tcgggcggtc tcgcgatccc ggcgctccac accgggctcg gcggcctctt
      481 cccccgctac gatgccccga accccgtcgc cgtcgcgctc ggacccggcg gcctcttcgc
      541 gctcgtcctc tccacgggcc tcgcgctctt tgccaccgtc gcggcgtcgc aagaactgcg
      601 gacgcttctc ggcgcgctca cgcacgtgcg ggttgggacg tggactccgc tcggagggtt
      661 cctctgcgcg gccgctgtcg cggccgcggt tccgatggct ctggcgggcc gctcgctcga
      721 acggcaagac ctcagcatcg ggtgaccttc taagaagatt ttctcttctc ttctcttcag
      781 ccaaccggat tcagcggcgg ttcgcccgcg aagaagcgga cgatctcggt gcacgcgaga
      841 cgcgccatcg cgaggcgcgt ctcggtcgtc gcagacccga ggtgcgggag gaggacgacg
      901 ttcgggagcc cgagaagggt cgcctcgatt tgcggctcgc gctggaacac gtcgaggccg
      961 gcgccgaaga gatgcttcga atgaagggcc gcggccagcg ccttttcatc cacgatcggg
     1021 cctcgcgcgg tgttgacgag gatcgtcccc ttcttcattc gaagaaactc tttctctccg
     1081 agtcttccgc gagtctcctc ggtcaaaggc gcatgaatcg acagcacatc cacttccgaa
     1141 agaaactctt cgaattcctc ttccgtcccg tccttctcga acgaaatcac gtccatcccg
     1201 aacgcctccg cccggagcgc aacggcgcgg ccgatgcggc ccatgccgta gatgcccagg
     1261 gtcttccccg cgagggagac gccgagcagc tcctgcggct tccagccgtc ccacgtgccc
     1321 gagcgggcca gcgcttcgcc ctcgtgcatg cggcgcgcga cggcgagaat cagagcgaag
     1381 gcgaggtcgg ccgtcgcgtc cgtgaggacg tccggcgtgt tcgtgacgag gacgccgcgg
     1441 gcgcggcacg ccgcgagatc cacgtggtcg acgccgaccg aatggctcgc gacgatgcgc
     1501 acgcgcggcg cggcggcgag gacctcctcg gtgatccgct cgcgcggcgt cgggaggagc
     1561 gcgtccgcgt ccgcgaggag cggccactcg gcgcgcggca ggctgccggc gccggtgacg
     1621 acggcgtgtc cggcgaggag cgcctgcgtc gaaggctcga cgagcggctg gcccacgaag
     1681 accttcatga cgacttgtac ggccggacga ggagccagcc ggcgacgaag accccgccgc
     1741 ccgcgaagaa catccgctcc tcgtacctca ggtcgtcgcg gaagaggccg acggcgagcc
     1801 cgacgcccac gaggacgatt ccgacgagct gcagcgcgcg gccgatcgcg aacctcattc
     1861 cacggcgccc gggggccgcg caggccgcgg ccgttccgtg acccggacgg agacgagcgc
     1921 gtcgtcctgc tcggtccggt cgaagaggtc catcccgctt cgcacgccgc cgaacacggt
     1981 gtagccgccg tcgaggtgaa tctggggcgc gagcgtcacg aaccactgcg agccgcccgt
     2041 gtcgggcccc gacaaggcca tgccgacggc gccgcgcgcg tagcggaggg gattgatctc
     2101 gtcgcggatc gcataacccg ggcctccgct cccgtcgccg cgcggatcgc ccgtctggac
     2161 gacgaagtcg ggcacgacgc ggtggatcac gatgccgtcg aagaagcgtt tcgcggcgag
     2221 agcgcggaaa ctctcgaccg tgatcggagc ggcctcggcg tcgagatcga ggacggccgt
     2281 cccccgcgcc gtctcgaccg tcgccgagaa cgacgactcg ttggcggctc gggcgagacg
     2341 gacgtagtcc gcgcccgtca gctgcgtctt caccggaatc gggcggaacg aagcggcctc
     2401 ggcaccgtat ttctcgacga gaacgcggcg cgccttgtcc cgcgtcacgg catccgcgtc
     2461 gttcgcgtgc gccgcgacga gcgccctgcc cgcctcgccc cgcgcggccg cggcgtcgag
     2521 cgcgccgacc gtgaagtccg gctcgttcga agaaaaggcg ccgtcgaacg cggatttcca
     2581 cgcgcggcgg agctccacgc tccccttctc gaggagcgga gccgcctcct cgagcgcgac
     2641 ctcgcgcacg gcgggatccc ggtcgatcag cgccgcgagc agccacgcgt ttcgcttcgc
     2701 cgtttttttc tttgaaagcg aagagactgc ggcggcgcgc acccgggcgc tgggatcgga
     2761 gagcagggcg tccgcaagag ggtcactcga aacaatcccc ctctttccct cccccgtctt
     2821 ctcgtcgtcc ccgaggaatt ccagcgcctc ggcggcgccg aggcgctgct cgagcgtccc
     2881 gccgagaatc tgcttctcga gccgcgcgga ggccttgtcc ggcgcccgtg ctgcgtcgag
     2941 tcgcgtcagg gcgacgaggg ccgactgccc tctccacccc tttcgaagca ggttctcttc
     3001 gagcgccgcg cgcgactccg gcgaatccgg cagcgcaccg aggaggcgga gagcggcgag
     3061 cgccacgccg gggagggagt catttgcacg gaggagagcg gcgctccggg tggttcgggt
     3121 cagatctgag gattcggaaa acagattctt cttagaaagt gaatacagcg cgcccagggc
     3181 ctgcaccgca accgagacgc ctgggctgcg ggcgagtgtg acgagatccg ctgcactttc
     3241 ttcgtctccg agaatcccca acccccgcgc cgcccaagcg acctcgtcgc tcgagggcgt
     3301 cgccgccccc tctcccttca ttcgaagaac cgcgcgaagc gcgggcgccg cttcaggctg
     3361 gggcttgcgc gacagggaat agacggccgc gcgcctcagc tcgggagcaa gagagttctc
     3421 ttcgaacacc gtcacgagag ccgagaccgt cctcgcctcg ggcttgcgga acatcgcgag
     3481 cgcgctcgcg gctcgcgtcg acgtggcgcc gtcgcgcgct gcgagagcgg ccaggagcgc
     3541 gtccgtcgcg cccttgccga gcccgaaggc gaag
//
LOCUS       DAOMFY010000348         3537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10562_length_3537_cov_7.588168, whole genome shotgun sequence.
ACCESSION   DAOMFY010000348 DAOMFY010000000
VERSION     DAOMFY010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10562_length_3537_cov_7.588168"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLB01_18320"
     CDS             complement(<1..257)
                     /locus_tag="PLB01_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79311.1"
                     /translation="MKNNNDLTGGSKVKAGFYFNKNNWEIVTVSGKKGGVLPGDAKAD
                     YLRVPAVAMLAGAPVLGAAFVVFLPVIGFALLATAAFNKAFR"
     gene            complement(451..2565)
                     /locus_tag="PLB01_18325"
     CDS             complement(451..2565)
                     /locus_tag="PLB01_18325"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQQ79312.1"
                     /translation="MAAPEAPPALVAVLSEIARVAGETLDLPEVFHRIATAAGRVLTF
                     DAVTVTLTDEADGFVLYSIGGAEVSDETRRSYARLVPRREYSPGLWATLTNTGVIDDV
                     VASCDRAFPRDRELSDSGIRAMLRAPLKTGARAMGYLSLLSLAPKAFRPSDMDLLAPI
                     ADIVAMAVAHARLAAGEQDRRRRYEALQALSPVLARALDVREVFDAISEIVRPVVPHD
                     RMAIGLLNEDRTAVNVYAVTGTGFPEVREPIRLSPQECARVNDWSTEIVRDVHTEVPP
                     DSGKSRHLAADGVRSLLRVPIFFEGQWQFAGGLLFLSRTPGTYGESDVPLAQHVADQI
                     ALALSHERLAGEARRAAEALSEAKKLERRVASLTEELASREGFGRIVGVAKSWKGILV
                     QVSKVAPTETTVLLTGESGTGKEILARAIHRASSRASGPFVAINAAALPDQLLESELF
                     GYERGAFTGATGGKPGRIEQAAGGTLFLDEVGEMSPAVQAKLLRVLQEREFQRLGGTR
                     VLKTDARMVAATNRDLKKAIARGDFREDLFYRLSVFEIALPPLRERVEDILPLTEVFL
                     AELGPAIGRPVAGVSKEAKDLLLAYPWPGNVRELKNALERAAILCDGNLITAAHLPIS
                     VSGHEASRPMRPEPPGERRPFPPGGVDLESLEKEYVKEALAKARFNKSKAARLLGLTR
                     AQLYSRIEKYGLGGGEEDKEFS"
     gene            2611..>3537
                     /gene="meaB"
                     /locus_tag="PLB01_18330"
     CDS             2611..>3537
                     /gene="meaB"
                     /locus_tag="PLB01_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQQ79313.1"
                     /translation="MPVPASAAALAEGVLLGRVPDVARAISWAESADARFPELLAALF
                     PKTGRARVTGLTGSPGAGKSTLTAALARRARARGRKVGIVAVDPSSPFSGGAILGDRI
                     RMQDLYTDPGVLIRSMATRGHLGGLARASADAVDVLDAAGFDEVLVETVGVGQDEVDV
                     FRLAESCVVVLTPGMGDDIQAIKAGLMEVADLFVVNKADRDGADRVVQEILQMLELGE
                     HGAWIPPVVKTVATTGAGLDELEAKLGEHRAFLDGPHGAVRRRDRTVKRIEGLVREDF
                     LRRVERLRGDSGTLDEVAGRVERREEDPISAAR"
BASE COUNT          508 a         1204 c         1308 g          517 t
ORIGIN      
        1 cggaaggcct tgttgaaggc ggccgtcgcg aggagcgcga agccgatgac ggggaggaag
       61 acgacgaagg ccgcgccgag cacgggagcg ccggcgagca tcgcgacggc cgggaccctg
      121 aggtagtcgg ccttcgcgtc gccgggcagc acgccgccct tcttgccgct gaccgtcacg
      181 atctcccagt tgttcttgtt gaagtagaag ccggccttga ccttggagcc gccggtgagg
      241 tcgttgttgt ttttcatgtc gtcccccttt gtctctttcc accgtgaaga tggggccggg
      301 ggccgacgga agcactcggg aaaagaggcc gaggagaggg gaaaaagtcc ggagagggga
      361 cagggagaat tccttagagg gtgaagagcg cgggacgcgg ccggagccgg gcgggcgacc
      421 ggcttgcgcc tccggcgccc ctaacccttt ctaagaaaac tctttatctt cctctcctcc
      481 gcccaatccg tacttctcga tcctcgaata cagctgcgcg cgggtgaggc cgaggagcct
      541 cgcggccttg ctcttgttga agcgcgcctt cgcgagcgcc tctttcacgt attccttctc
      601 cagcgactcg aggtccacgc cgccgggcgg aaacgggcgt cgttcgccgg gcggctccgg
      661 cctcatcgga cgcgaggcct cgtggccgga cacggagatc gggaggtggg cggccgtgat
      721 gaggttgccg tcgcagagga tcgcggcgcg ctcgagcgcg ttcttgagct cgcggacgtt
      781 gccgggccac gggtaggcga ggaggaggtc cttcgcctcc ttcgagacgc ctgcgacggg
      841 gcggccgatc gcagggccca gctcggcgag gaacacctcc gtgagcggga ggatgtcctc
      901 gacgcgctcg cggagcggcg gcagggcgat ctcgaagacc gagagacgat agaagaggtc
      961 ctcccggaaa tctccgcgtg cgatcgcctt cttcaggtcg cggttcgtcg cggccaccat
     1021 gcgcgcgtcc gtcttgagca cccgggtccc gcccagccgc tggaactccc gctcctgcag
     1081 gacgcgcagg agcttcgcct gcacggccgg gctcatctcg cccacttcgt cgaggaagag
     1141 cgtgccgccg gccgcctgct cgatgcgtcc cggcttgccg cccgtcgccc ccgtgaaggc
     1201 gccgcgctcg tagccgaaga gctcggactc gaggagctga tccgggagcg ccgccgcgtt
     1261 gatcgcgacg aacggcccgg aagcccgcga cgaggcgcgg tggatcgcgc gcgcgaggat
     1321 ctccttgccc gtgccggact cgcccgtgag gaggacggtc gtctccgtcg gcgcgacctt
     1381 cgagacctgg acgaggatgc ccttccagct cttcgcgacg ccgacgatcc ggccgaagcc
     1441 ctcgcgcgaa gcgagttcct ccgtgaggga cgcgacgcgc cgctcgagtt tcttcgcctc
     1501 gctgagcgcc tccgcggcgc ggcgtgcctc gcccgcgagg cgctcgtgcg aaagcgccag
     1561 cgcgatctgg tcggcgacgt gctgggccag ggggacgtcg ctctcgccgt acgtgcccgg
     1621 agtccgggag aggaacagga gccctcccgc gaactgccac tggccttcga agaagatcgg
     1681 gaccctgagg agcgaccgca caccgtcggc cgcgagatgc cggctcttgc ccgagtcggg
     1741 agggacttcc gtgtggacgt cccgcacgat ctcggtggac cagtcgttca cgcgcgcgca
     1801 ttcctgcggc gagagccgga tcggctcgcg aacctccggg aaccccgttc ccgtgaccgc
     1861 gtagacgttg acggccgtgc ggtcctcgtt caggaggccg atcgccatgc ggtcgtgcgg
     1921 gacgaccggg cggacgatct ccgagatcgc gtcgaagact tcccgcacgt cgagcgcccg
     1981 cgcgaggacg ggggagagcg cctggagcgc ctcgtaccgc cgccgccggt cctgttcccc
     2041 cgccgcgagg cgcgcgtggg cgacggccat cgcgacgatg tccgcgatcg gggcgagaag
     2101 gtccatgtcg gagggtcgga acgccttcgg ggcgaggctc aggagcgaga gataacccat
     2161 ggcccgcgcg cccgtcttga gcggcgcccg gagcatcgcg cggatgccgg agtccgagag
     2221 ctcgcggtcg cgcgggaacg cgcggtcgca gctcgcgacg acgtcgtcga tgacgcccgt
     2281 gttcgtgagg gtcgcccaca gcccgggcga gtactcgcgg cgcggcacga ggcgggcgta
     2341 cgagcgtcgc gtttcgtccg agacctcggc gccgccgatc gagtagagga cgaacccgtc
     2401 cgcctcgtcc gtcagcgtga ccgtcaccgc gtcgaacgtc aggacgcgcc cggccgccgt
     2461 cgcgatccgg tggaagacct cggggaggtc cagcgtctcg cccgcgacgc gggcgatctc
     2521 ggacaggacg gccaccaggg cgggaggggc ttcgggcgcg gccatggggg agtcattcta
     2581 ggtcccgcgc ctcgtctaca ctcgggcgga atgccggttc ccgccagcgc cgccgccctc
     2641 gccgaagggg tgctcctcgg ccgcgtgccc gacgtcgcgc gggcgatctc gtgggccgag
     2701 agcgccgacg cccgctttcc cgagcttctc gccgccctct tcccgaagac gggccgcgcc
     2761 cgcgtcacgg gcctcacggg ctcgccgggg gcgggcaagt ccacgctgac cgcggccctc
     2821 gcccggcgcg cgcgcgcccg cgggcggaag gtcgggatcg tcgcggtgga cccgtcgtcg
     2881 cccttctcgg gcggcgcgat cctcggcgac cgcatccgga tgcaggacct ctacacggac
     2941 cccggcgtcc tgatccgctc gatggcgacg cgcggccatc tcggcgggct cgcgcgcgcc
     3001 tcggcggacg ccgtcgacgt cctcgacgcg gcgggcttcg acgaggtgct cgtcgagacg
     3061 gtcggcgtcg ggcaggacga ggtggacgtc ttccgcctcg cggaatcgtg cgtcgtcgtc
     3121 ctgacgccgg ggatgggcga cgacatccag gcgatcaagg cgggcctcat ggaggtcgcg
     3181 gacctcttcg tcgtgaacaa ggccgaccgc gacggcgccg accgcgtcgt gcaggagatc
     3241 ctccagatgc tcgagctcgg cgagcacggc gcgtggatcc cgccggtcgt gaagaccgtc
     3301 gcgacgacgg gcgcgggcct cgacgagctg gaggcgaagc tcggggagca tcgggctttt
     3361 ctggatgggc cccacggcgc cgtcaggcgc cgcgaccgca cggtgaagcg aatcgaaggc
     3421 ttggtcagag aagattttct tagaagggtc gagagactgc ggggggacag cggaacgctc
     3481 gacgaggtgg cggggcgcgt cgagcggcgc gaagaggacc cgatttcggc ggcgcgc
//
LOCUS       DAOMFY010000349         3512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10698_length_3512_cov_8.785074, whole genome shotgun sequence.
ACCESSION   DAOMFY010000349 DAOMFY010000000
VERSION     DAOMFY010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10698_length_3512_cov_8.785074"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLB01_18335"
     CDS             <1..243
                     /locus_tag="PLB01_18335"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQQ79314.1"
                     /translation="GVPEDPATGSAAGPVGVYLALHGAVPGGVAGGRGRFLIDQGVEM
                     GRPSELDVTVLVDGGRPTGVRVEGGAVLVMRGELDV"
     gene            324..860
                     /locus_tag="PLB01_18340"
     CDS             324..860
                     /locus_tag="PLB01_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79315.1"
                     /translation="MNDPRAILLSLSSLALLACSTVSIESQVHSTPAPAFSRTLVVVA
                     LPDAVRRPAEDALVARLASLHARTSYEHVPLAPGVTLGALREAARRDGFDGLLVVWPA
                     DMTSKTYPSLGNNSNFPVTNSLQVGMRLVASLTSLGDDREIWKGVVTNRDAAPLVKGT
                     PKMAASLADRLVSDGVAN"
     gene            915..2378
                     /locus_tag="PLB01_18345"
     CDS             915..2378
                     /locus_tag="PLB01_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQQ79316.1"
                     /translation="MNPIRSRLGAAFALLVVLPFSFALSSRSEAPGSVAESLDRYLSA
                     RTALGRFSGAVLVAKGDRVLFRKGYGFSDVARRTPYTPETRHAVASITKMFTAMAALK
                     LRDAGTLKLEDSICAHLAGCPEAWKRVTVQQLMRHTSGIPDYESPLELGSEKYLSVMT
                     REGTSRRLVEDAKTRPLDFPPGTKFRYSNTGYLVLAQVVENAAGRPFNEFVAETLLKP
                     AGMTRAGLFDGKAVPDGLAVGYTHPEIGWAKLLAGAPLTDGHLAAQPRLPLTPPAGDA
                     GLYATADDLLAWSRAMDGSALVPKAEADEVFTPGLEHYGYGWFVGEGWKRRRFRHNGG
                     LPGYTSDLVKFPDEGLTLVFLCNLDRARLSSVVRDVTAIVLGEPWDMPVSGPVATLAP
                     ADYARLEGVYATADGKKLTVKKEKELLTAALEGRYTAGLIPLSPTRFYFPLGDGTATF
                     SLGEDGRASSVNVRYSAEDHPATRLSPSASPPASSHP"
     gene            complement(2639..3498)
                     /locus_tag="PLB01_18350"
                     /pseudo
     CDS             complement(2639..3498)
                     /locus_tag="PLB01_18350"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          488 a         1273 c         1180 g          571 t
ORIGIN      
        1 ggcgtgccgg aggaccccgc gacgggctcg gcggccgggc ccgtgggcgt ctacctcgcg
       61 ctccacggcg cggtgccggg cggcgtcgcg ggcgggcgcg gccgtttcct catcgaccag
      121 ggcgtcgaga tgggccgccc gtccgagctg gacgtgacgg tcctcgtcga cggcggccgg
      181 ccaaccggcg tgcgcgtcga gggcggagcg gtcctggtga tgaggggaga gctcgacgtc
      241 tgaacccgcc tccgcggcac aggaaatgct gcccctgcgg gagtagtctc tattctgaga
      301 cgacccggaa aaagaaggcg ctgatgaacg acccgcgcgc gatcctcctc tcgttgtcct
      361 ctctcgccct cctcgcctgc tccaccgttt cgatcgagag ccaggtccac tcgacgcccg
      421 ctcccgcgtt ctcgcgcacg ctcgtcgtcg tggccctccc ggacgccgtg cggcgccccg
      481 ccgaggacgc gctcgtggcg cgcctcgcgt ccctgcacgc gcggacatcg tacgagcacg
      541 tgcccctcgc gcccggcgtc acgctgggcg ccctgcgcga ggccgcgcgc cgggacggct
      601 tcgacgggct cctcgtcgtc tggcccgccg acatgacgag caagacgtac ccctcgctcg
      661 gcaacaacag caatttcccg gtcaccaact ccctgcaggt cggaatgcgg ctcgtcgcga
      721 gcctcacatc cctcggcgac gaccgggaga tctggaaggg cgtcgtcacg aaccgcgacg
      781 ccgcccctct cgtcaagggg accccgaaga tggcggcgag cctcgccgat cgactcgtct
      841 ccgacggcgt cgcgaattga ggcccgaccc gggccccgta acctcgtgac cgcgaggaac
      901 gtctcccggt cacgatgaac ccgatccgct cccgtctcgg cgcggccttc gcgctcctcg
      961 tcgttctgcc cttttccttc gcgctttcct cccgctcgga agcccccggc tccgtcgcgg
     1021 agtccctcga ccggtacctc tcggcccgga cggccctcgg ccggttcagc ggcgccgtgc
     1081 tcgtcgcgaa gggcgaccgc gtcctcttcc gcaagggcta cgggttctcc gacgtggccc
     1141 gccggacgcc gtacacgccg gagacccggc atgcggtcgc ctccatcacg aagatgttca
     1201 cggcgatggc ggcgctcaag ctgcgcgacg ccggaactct gaagctcgag gattcgatct
     1261 gcgcgcacct cgcgggatgt cccgaggcct ggaagcgcgt cacggtgcag cagctcatgc
     1321 gtcacacgtc cggcatcccc gactacgaga gcccgctcga gctcggttcc gagaaatacc
     1381 tctccgtcat gacgcgggag ggcaccagcc ggcgcctcgt ggaggacgcg aagacgcggc
     1441 cgctcgactt tccgcccggc acgaagttcc gttacagcaa cacgggctac ctcgtcctcg
     1501 cgcaggtcgt cgaaaacgcg gcgggccggc ccttcaacga gttcgtcgcg gagacgctcc
     1561 tgaagccggc aggcatgaca cgcgcggggc tgttcgacgg gaaggccgtt cccgacggcc
     1621 ttgccgtcgg ctacacgcac cccgagatcg ggtgggcgaa actcctcgcg ggggcgccgc
     1681 tcacggacgg gcacctcgcg gcgcagccgc gcctcccgct cacgccgccc gccggcgacg
     1741 ccggcctcta cgcgaccgcc gacgaccttc tcgcgtggag ccgtgcgatg gacggaagtg
     1801 cgctcgtgcc gaaggcggag gccgacgagg tgttcacgcc cggcctcgag cactacggct
     1861 acggctggtt cgtcggcgag ggctggaagc gccgccggtt ccggcacaac ggaggcctgc
     1921 cgggctacac ctccgacctc gtgaagttcc cggacgaggg cctcacgctg gtcttcctct
     1981 gcaacctcga ccgcgcgcgc ctctcgtccg tcgtccgcga cgtgacggcg atcgtcctcg
     2041 gcgagccgtg ggacatgccg gtctcgggtc cggtcgcgac gctcgcaccc gcggactacg
     2101 cgcgcctcga gggtgtttac gcgacggcgg acgggaagaa actgaccgtc aagaaggaga
     2161 aggaactcct cacggccgcg ctcgaaggac gttacacggc gggactcatc ccgctctccc
     2221 cgacgcgctt ctacttcccg ctcggggacg ggacggctac tttttcgctc ggcgaggacg
     2281 ggcgggcttc ttcggtgaac gtgcggtact cggcggagga ccatccggcg acgcggcttt
     2341 cgccttcggc ttcgccgccc gcttcttcgc acccttgacg ggcgcgggca cgcgcgcctc
     2401 ggcgatcgcg gcctcgagcg cgccctcggg gatgcgcgtc gtgggctcga agaggtcgag
     2461 gtcgctcgcg ggaacgtcga ggccgcagcc ctcggtttcg cagacgtaga acgcgcccgc
     2521 cttgcggtcg cgcacgagaa ggtacggcgc cttgcagttc gggcacgcct tcggaacggg
     2581 ccgcgccttg aagttcttct cgcacgacgg gtaattcgag cacccgtaga acgggccgaa
     2641 gcgcccgcgc cgcacgagga tgtcgcccgt cgtgcacacc gggcacttga cgcccgtgag
     2701 cgtgacgggc ttgggcggct tgaagcggca ggccgggtag ttcgagcagg agacgtagtc
     2761 gccgaaacgg ccgtggcgcg tcacgagggc cgagccgcag ttcgggcact tttcgccggt
     2821 cggaacgtcc ggcttcgcct cggccttccc gcccgcgacc ttcacgggca ccgtgttgcg
     2881 gcagaccggg taggccgtgc agccgaggaa cgcggagccg ctgcgtcccg tgcggagctt
     2941 catcgcgctg ccgcactcct cgcaggtctg tccctcgagt tcggtcgcgt cgatcgggtc
     3001 ttcctcgacc tcggggatgt ccagggtgaa gtcgcagtcg gggtagcccg agcacgcgat
     3061 gaagaggccc gccttcccca tccggagctt gagcgcgtgg cccgtcttcg ggcacgtgtc
     3121 gcccgggtcg ttcgtgagct tgaagtccgt gaagtgagtc ctcacgtcct tgagaggcag
     3181 gcccgccttg agcgagacca tcttcttctt cgccttctcg cggtccttga cgaacttgcc
     3241 gtcgaactcc gcgagcgcct tgcgccagtc gagctttcct tcctcgacct cgtcgagctc
     3301 ctcttccatc cgcgccgtgt actcggtctg gaagaagtcg tcgaagccct cgcgcatgag
     3361 gtccacgatg acctggccga gcggcgtcgg cgtgaagcgg cggtccttct tgcgcacgta
     3421 ctcacgatcc tcgatcttgc gcaggatctc ggcgtacgtg gacgggcggc cgatgccgtt
     3481 ctcctcgagg aacttcacga gcgacgcctc gt
//
LOCUS       DAOMFY010000350         3477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10875_length_3477_cov_7.819404, whole genome shotgun sequence.
ACCESSION   DAOMFY010000350 DAOMFY010000000
VERSION     DAOMFY010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10875_length_3477_cov_7.819404"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLB01_18355"
     CDS             complement(<1..441)
                     /locus_tag="PLB01_18355"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQQ79317.1"
                     /translation="MADAVNLAILGGGAAGLLAAIRAAEAGASGVVVLEAAPQAGRKI
                     LVSGGGRCNVLPMEEARERFVSGAPPRVVGRYLARVPLDEQRAFFEDLLGGPLREEPE
                     GKKLFPPSNRAKDVRDALAARALAAGATIRTGTVVRELGRTDGGF"
     gene            459..1247
                     /locus_tag="PLB01_18360"
     CDS             459..1247
                     /locus_tag="PLB01_18360"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQQ79318.1"
                     /translation="MPHSTGGPGGFLRRIGYRVYFVLACAVGGLSFILFAAVALFHYA
                     LFRNGEKAALVWIRSFGFVMTRFLFWNVAVENLNTLLATRPAVVVGNHQSNLDIATWA
                     TFFPDRSVAVGKKEILKIPVFGWLWKVSKHILIDRSNAIAARESLKAAAERIRTEDLS
                     VWVLPEGHRNSKPEMLPFKKGAFHLAIAAQVPVVPFATSPMWEVLDAHRWMVRPGTVR
                     VRFLPPIPTAGMTDDDVDRLSLAARAAIEGARQDFLKSAGPRIG"
     gene            complement(1244..1681)
                     /locus_tag="PLB01_18365"
     CDS             complement(1244..1681)
                     /locus_tag="PLB01_18365"
                     /inference="COORDINATES: protein motif:HMM:NF040124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HQQ79319.1"
                     /translation="MAETPTQDFRTHRRWDPWYHFFAAPVLVIAFFVHLWLAIKAPSA
                     WTIWGIFVSAALVVIAWKARSFALRAQDRVIRLEERERLGSVLDGPLRARSLSLTEDQ
                     LIGLRFASDAELAVLVQAALDENLSGEAIKKRIRSWRPDFFRV"
     gene            1788..2444
                     /locus_tag="PLB01_18370"
     CDS             1788..2444
                     /locus_tag="PLB01_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQQ79320.1"
                     /translation="MFRPRALAPLALALVSFTGTSAHAEKETFVLDKNHTVFGFKVRH
                     FVTKVPGRFAKFEGTIVLDRAKPEESSVDLRVDATSIDTGVPNRDKHLNSADFFDTAK
                     FPEITFKSTKIAAKGKDAYEVTGDLTMRGVTKPVTLAVTANGFANDGRGGQKTGFDVA
                     GKLNRKDFGVNWNATVDQNLMLSDEVDLEISVEANKPAPKPAAPPAAPAAAPAAPATK
                     "
     gene            2537..>3477
                     /locus_tag="PLB01_18375"
     CDS             2537..>3477
                     /locus_tag="PLB01_18375"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQQ79321.1"
                     /translation="MTEIPPPPAPFRSHRVRPIAAFGFALVHVAAVVGVVTLGLSWKG
                     ALLAVASYYLRMFAITAGFHRYFSHKAFRLSRVPQFLLAFLGQTAAQKGVLWWASNHR
                     HHHKYSDTPEDIHSPLQDGFWWSHIGWILSGKYDKTDFSRIPDLAKFPELVWLDRNQY
                     GPTMLYGLLSWALFGWTGLVWGYFVSTVLLWHGTFTINSVMHVFGRRVFPTTDTSRNS
                     MIFALVTMGEGWHNNHHFYPSSAAQGFRWWQLDASFYILWLGEKLGVVKSLRRAPTAA
                     VIARLTSGAGAVASPAHLASDAADALAVRWEEIRSAA"
BASE COUNT          543 a         1267 c         1120 g          547 t
ORIGIN      
        1 gaagcccccg tccgtccgtc cgagctcgcg cacgacggtg cccgtccgga tcgtcgcgcc
       61 ggccgcgaga gcccgcgccg cgagcgcgtc gcgcacgtcc ttcgcgcggt tcgacggcgg
      121 gaagagcttc ttgccctcgg gctcctcgcg cagcggcccg ccgaggaggt cctcgaagaa
      181 tgcgcgctgc tcgtcgagcg gaacgcgcgc gaggtaccgg cccacgacgc gcggcggcgc
      241 tcccgagacg aagcgctcgc gcgcctcctc catcgggagg acgttgcagc gccccccgcc
      301 ggagacgagg atcttccggc cggcctgcgg cgccgcctcc aggacgacga cgccggaggc
      361 gcccgcctcg gcggcgcgaa tcgccgcgag gagtccggcc gccccgcccc cgaggatcgc
      421 gagattcacc gcgtccgcca tcccgtcgat aatgcggcat gccccattcg accggcggtc
      481 ccggcgggtt cctccgccgt atcggctacc gcgtgtactt cgtcctcgcg tgcgcggtcg
      541 gaggcctctc cttcattctc ttcgccgccg tcgcgctctt ccattacgcg ctcttccgca
      601 acggcgagaa ggcggctctc gtctggatcc ggagcttcgg tttcgtgatg acgcgtttcc
      661 tcttctggaa cgtggccgtc gagaacttga acacgctgct cgcgacgcgg cccgccgtcg
      721 tggtcgggaa ccaccagtcg aatctcgaca tcgcgacgtg ggcgacgttc ttccccgacc
      781 ggagcgtcgc cgtcggaaag aaggagatcc tcaagatccc cgtcttcggc tggctgtgga
      841 aggtgtcgaa gcacatcctg atcgaccgct ccaacgccat cgcggcgcgc gagtcgctga
      901 aggcggccgc cgagcgcatc cggacggaag acctgagcgt ctgggtgctg cccgaagggc
      961 accggaattc gaagcccgag atgcttccgt tcaagaaggg cgccttccac ctcgcgatcg
     1021 ccgcgcaggt gccggtggtc ccgttcgcga cctcgccgat gtgggaggtc ctcgacgcgc
     1081 accgctggat ggtgcggccc gggaccgtgc gcgtgcggtt cctgccgccg atcccgacgg
     1141 ccgggatgac ggacgacgac gtggaccgcc tcagcctcgc cgcacgcgcg gcgatcgagg
     1201 gggcccgcca ggacttcctg aagtcggcgg gcccgcggat cggctagacg cggaagaagt
     1261 cgggccgcca gctccggatc cgcttcttga tcgcctctcc gctgaggttc tcgtcgagcg
     1321 cggcctggac gaggaccgcg agctcggcgt cggaggcgaa tcgcaggccg atgagctggt
     1381 cttccgtgag cgagaggctc cgggcgcgca gcggcccgtc gaggaccgac ccgaggcgct
     1441 cccgctcctc gagccggatg acgcggtcct gggccctgag cgcgaacgac cgggccttcc
     1501 acgcgatcac gacgagggcc gccgagacga aaatccccca gatcgtccac gcggacggcg
     1561 ccttgatcgc gagccagagg tggacgaaga aggcgatcac gagaacgggg gccgcgaaga
     1621 agtggtacca ggggtcccag cggcggtggg tcctgaagtc ctgcgtgggc gtttcggcca
     1681 tgtctttcct ctccgtgcaa tccgtcgccg ctccggcttc ggatcctcga tagaatcgcg
     1741 gccccccggg gctccgcccc actctatcca aaaggagact cccccgcatg ttccgacccc
     1801 gtgccctcgc gccgctggcg ctggccctcg tctctttcac gggcacctcc gcgcacgccg
     1861 agaaagagac cttcgtcctc gacaagaacc acaccgtgtt cggattcaag gtccgccact
     1921 tcgtgaccaa ggtccccggc aggtttgcga aattcgaggg cacgatcgtc ctcgatcgcg
     1981 cgaaaccgga ggagagctcc gtcgacctca gggtcgacgc gacgagcatc gacacgggcg
     2041 tgccgaaccg cgacaaacac ctcaactcgg ccgatttctt cgacaccgcg aagttccccg
     2101 agatcacctt caagtccacg aagatcgccg cgaagggcaa ggacgcctac gaggtcaccg
     2161 gcgacctcac gatgcgcggc gtcacgaagc ccgtcacgct cgccgtgacc gcgaacggct
     2221 tcgcaaacga cggacgcggc ggccagaaga ccggcttcga cgtcgccgga aagctcaacc
     2281 gcaaggactt cggcgtgaac tggaacgcga ccgtggacca gaacctgatg ctctcggacg
     2341 aggtggatct cgagatctcg gtcgaggcga acaaacccgc ccccaagccg gcggccccac
     2401 ccgcggcgcc cgcggccgcc cccgcagccc ctgccacgaa atgaccgatt ccccgctttg
     2461 tgcgggagca ccaagagctt aggattcaat cgcccggcct cgcggccggg cgaatcacct
     2521 tcgaaggaac tctccgatga ccgaaattcc tcccccgccg gcgccgttcc ggagccaccg
     2581 cgtccgcccg atcgcggcgt tcggcttcgc cctcgtccac gtcgccgcgg tcgtcggcgt
     2641 ggtgacgctc ggcctctcgt ggaagggcgc cctgctcgcg gtcgcctcgt actacctgcg
     2701 gatgttcgcg attacggcgg gtttccaccg ctacttctct cacaaggcct tccggctgtc
     2761 gcgcgttccg cagttcctcc tcgccttcct cggacagacc gccgcgcaga agggcgtgct
     2821 gtggtgggcg tcgaaccacc gccaccacca caagtactcc gacacacccg aggacattca
     2881 cagccccctt caggacggct tctggtggag ccacatcggc tggatcctct cgggcaagta
     2941 cgacaagacg gacttctcgc ggatcccgga cctcgcgaag ttccccgagc tcgtctggct
     3001 ggaccgcaac cagtacggcc cgacgatgct ctacggcctt ctctcgtggg cccttttcgg
     3061 ctggacgggc ctcgtgtggg gctatttcgt ctcgaccgtt ctcctctggc acgggacgtt
     3121 cacgatcaac tccgtcatgc acgtcttcgg gcgtcgcgtc ttcccgacga cggacacgag
     3181 ccgcaacagc atgatcttcg ccctcgtcac gatgggagaa ggctggcaca acaaccatca
     3241 cttctacccg agctcggccg cgcagggctt ccgctggtgg cagctggacg cgagcttcta
     3301 catcctctgg ctgggcgaga agctcggcgt cgtcaagagc ctgcgccgtg cgccgacggc
     3361 cgccgtcatc gcgcgcctga cgtccggagc gggcgccgtg gcctcgccgg cgcacctcgc
     3421 gtccgacgcc gcggacgcgc tcgcggtgcg gtgggaggag atccgctcgg cggccag
//
LOCUS       DAOMFY010000351         3473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10894_length_3473_cov_7.061439, whole genome shotgun sequence.
ACCESSION   DAOMFY010000351 DAOMFY010000000
VERSION     DAOMFY010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10894_length_3473_cov_7.061439"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLB01_18380"
     CDS             complement(<1..373)
                     /locus_tag="PLB01_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQQ79322.1"
                     /translation="MATRDLYAALGIPKSASDADVKKAYRRLARKFHPDVNPGDATAK
                     KKFQEIASAYEVLKDAKRRKHYDLTGDTGEPPEPGAWPPGGGAPRGAARPAGSPFGGA
                     PGGGGPGGGFRWTGDFGDLFSE"
     gene            448..1692
                     /gene="glgC"
                     /locus_tag="PLB01_18385"
     CDS             448..1692
                     /gene="glgC"
                     /locus_tag="PLB01_18385"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008221398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQQ79323.1"
                     /translation="MLQHRDVLSVVLAGGMGERLFPLTRDRAKPAVPFGGRYRIIDFV
                     LNNFVNSGLTKVKVLTQFKSNSLIEHIIRTWRLTFDIGQFVDPVPAQMRRGPHWFRGT
                     ADAVYQNLDLVFDEEPAHVCVFGGDHIYVMDVNPMLAFHEDGGNDLTIAAFPVPLKEA
                     SRYGILEVAADGRIVGFEEKPAEPRAIPGQPDLALASMGNYIFRASVLIEALEHDALQ
                     DTTHDFGKNLIPSLLKGGARLYAYDFSTNRVPGNEDLAPYWRDVGTVDSYWDASMDLI
                     SVTPPLNLYNQKWPIKSHSPQLPPAKFVFADAVSQRIGLATDSLVASGGIISGGTIHR
                     SILFPRVRINSFSHVEECVLMDGVDVGRYARLKRVIADKGVRIPPRTEIGFDPEEDKK
                     RFHVSEGGVVVLPKGAVLSDPD"
     gene            1773..2231
                     /locus_tag="PLB01_18390"
     CDS             1773..2231
                     /locus_tag="PLB01_18390"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQQ79324.1"
                     /translation="MDDTDRRILDILQRDAKISQARIAETVGLTTPSVNERIKKLETK
                     GFILRFTALLDPRLVGMPLVAHVDVMLEHPRFERPFLEEIESLLDVQECYAISGDFGY
                     RLKIRARDVEHFERILREKLQTLRGVVRTRVELSLSIKKDSTLLPLAENA"
     gene            complement(2612..3211)
                     /locus_tag="PLB01_18395"
     CDS             complement(2612..3211)
                     /locus_tag="PLB01_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79325.1"
                     /translation="MRSLLRFAACLGLLAAALSARAQVGPPRTHQFPFAVGVGGEWVT
                     DNGRATDVDAFSNGGWHAFGEVVLETGVFLQARYMNFALPGTPVTPQFGVGTPTTAPD
                     VNVKAGVVSVGYLFREPWWDGGFFGGVGVYGLDPKPVGEGQSSTDIKETVIGWHGGVM
                     AAFHISARWDLRLEAAAYLLRTEANHKPITLGGSVAYHF"
BASE COUNT          570 a         1211 c         1113 g          579 t
ORIGIN      
        1 gctccgagaa gagatcgccg aaatcgccgg tccagcggaa cccgccgccg ggccctccgc
       61 cgcccggcgc gccgccgaac ggtgatcccg ccgggcgagc ggccccacgg ggggcgcctc
      121 ctccgggcgg ccatgcaccg ggctcgggcg gctcgcccgt gtctccggtc aggtcgtagt
      181 gcttgcgccg cttcgcgtcc ttgaggacct cgtacgccga cgcgatctcc tggaacttct
      241 tcttcgccgt cgcgtcgccg gggttgacgt cggggtggaa cttgcgcgcg aggcgtcgat
      301 acgccttctt gacgtcggcg tcggacgcgg atttcgggat tccgagcgcg gcgtagaggt
      361 cccgggtcgc catggcgcca cgatacccga gacgccgggc ctcgtcgcgg taacatggga
      421 gcgcccgttc gaggcaccgc cgtgaagatc ctccagcatc gagacgtgct ctccgtcgtc
      481 ctcgcgggcg gcatgggcga gaggctcttc ccgctcacgc gggaccgcgc caagcccgcc
      541 gtgcctttcg gcgggcgcta ccggatcatc gacttcgtcc tgaacaactt cgtgaactcc
      601 ggcctcacga aggtcaaggt gctcacgcag ttcaagtcga attcgctcat cgagcacatc
      661 atccgcacct ggcgcctcac gttcgacatc gggcagttcg tcgatccggt gcccgcgcag
      721 atgcgccgcg gcccgcactg gttccgcggc accgcggacg ccgtctacca gaatctcgac
      781 ctcgtgttcg acgaggagcc cgcgcacgtc tgcgtgttcg gcggtgacca catctacgtg
      841 atggacgtga acccgatgct cgcgttccac gaggacggcg gaaacgacct cacgatcgcc
      901 gccttcccgg tgccgctcaa ggaggcctcg cgctacggga tcctcgaggt cgccgcggac
      961 ggccggatcg tcggattcga ggagaagccc gcggagccgc gcgcgatccc cggacagccg
     1021 gatctcgcgc tcgcgtcgat ggggaactac atcttccgcg cgtccgtcct gatcgaggcg
     1081 ctcgagcacg acgcgctgca ggacacgact cacgacttcg gcaagaacct gatcccgtcc
     1141 ctgctgaaag gcggcgcgcg cctctacgcg tacgacttct ccacgaaccg cgtccccgga
     1201 aacgaggacc tcgcgccgta ctggcgggac gtcggcacgg tggattccta ctgggacgcg
     1261 tcgatggacc tcatctccgt cacgcctccg ctcaatctct acaaccagaa gtggcccatc
     1321 aagagccact ctccgcagct gccgcccgcg aagttcgttt tcgcggatgc ggtttcgcag
     1381 cgcatcggcc tcgccacgga ctcgctggtc gcgtcgggcg gcatcatctc gggcggcacg
     1441 atccaccggt ccatcctgtt cccgcgcgtg aggatcaact cgttctcgca cgtcgaggag
     1501 tgcgtcctga tggacggcgt ggacgtcggg cgctacgcgc gtctcaagcg cgtcatcgcc
     1561 gacaagggcg tgaggatccc gccgcgcacg gagatcggct tcgaccccga ggaggacaag
     1621 aagcgctttc acgtgtccga gggcggcgtc gtcgtcctgc ccaagggtgc ggtgctctcg
     1681 gaccccgact gacgctccgg ccccaattcc ggttgcctcg gcgacggtga ccgtataaag
     1741 ttcgggttct cgatcccaga gccgaatttt atatggacga caccgatcgc cgcatcctcg
     1801 acatcctcca gcgggacgcg aagatctcgc aggccaggat cgccgagacc gtgggcctga
     1861 ccacgccttc ggtgaacgag cggatcaaga agctcgagac gaagggcttc atcctgaggt
     1921 tcacggctct cctcgacccg aggctggtcg ggatgcccct cgtcgcccac gtggacgtga
     1981 tgctggaaca cccgcgcttc gaaaggcctt tcctcgagga aatcgagagc cttctcgacg
     2041 tccaggagtg ctacgcgatc tcgggcgact tcggctaccg gctgaagatc cgcgcccggg
     2101 acgtcgagca tttcgagaga atcctccggg aaaagctcca gacgctgcgc ggggtcgtgc
     2161 gcacgcgcgt cgagctctcg ctgtcgatca agaaggactc gacgctgctc cccctggccg
     2221 aaaacgcctg agaccccgtc tcgctccggg agtgtcgatt tcgcaccaaa tcgtcccctg
     2281 aaaggcgcgt ttcggagtgc cgatccggcg tgactttgcg cacagcgtaa tggcaagtca
     2341 ctgcaagggc ttcggttgga aacgatcgtt cgaggaagaa aaccgaaggc atcgcgcttg
     2401 cgtatacgaa ggcaggagat cgaaacgagc attcgatgca aagtcagatg aaaacgccgg
     2461 tcttcacttc cttccatctt cccgccggcc tcccggccga cgggccctcc acttctcttc
     2521 cctccacaca gtaaccctca gttagtccat cccaccgccg caatccccgc ccgaccgaaa
     2581 ggtcgggtgg gagcccacct tttctggcgg gctagaagtg gtaggcgacc gaaccgccca
     2641 gggtgatggg cttgtggttc gcttcggtgc gcaggaggta ggccgcggct tcgagccgga
     2701 ggtcccagcg cgcggagatg tggaacgcgg ccatgacgcc gccgtgccag ccgatgaccg
     2761 tttctttgat gtccgtcgag ctctggcctt cgccgaccgg cttgggatcc aggccgtaga
     2821 ccccgacgcc gccgaagaag cctccgtccc accacggctc gcggaacagg tagccgaccg
     2881 acacgacgcc cgccttcacg ttgacgtcgg gcgccgtcgt cggggtcccg acgccgaact
     2941 gcggggtgac gggcgtgccc ggcaacgcga agttcatgta gcgggcctgg aggaagacgc
     3001 ccgtctcgag gacgacctct ccgaacgcgt gccatccccc gttcgagaac gcatcgacgt
     3061 cggtggcacg gccgttgtcc gtcacccact cgccgccgac gccgacggca aacggaaact
     3121 ggtgcgtgcg gggcggcccg acctgggcgc gggcggacaa cgctgcggcg agaaggccga
     3181 ggcacgcggc gaatcgaagg agtgaacgca tcggtttcga gaatacttca ggtgcggcgg
     3241 cgccatgagt catatcgttc atacgatcgc cgcgccgccg tcgtctagaa tccgcgccgt
     3301 gcccggcccg gagccgcttc gacatctccc cggcgcgccc gcaatcgcct ctctcctcgc
     3361 gttgctcgtg ccctcccggt gcgccgtgtg cgacgaaccc cgccgcgacg caggaggcgg
     3421 aggcgtctgc cgcgcctgct gggccgcgct gccgcggctt gcgccctcca cag
//
LOCUS       DAOMFY010000352         3465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_10945_length_3465_cov_8.221408, whole genome shotgun sequence.
ACCESSION   DAOMFY010000352 DAOMFY010000000
VERSION     DAOMFY010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10945_length_3465_cov_8.221408"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..3357
                     /locus_tag="PLB01_18400"
     CDS             22..3357
                     /locus_tag="PLB01_18400"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQQ79326.1"
                     /translation="MKNRLSLFALLLPLCLLLASGPVLAAGPDGPPEARLLRYPDAWG
                     DFVVFTYAGDLWRAPLAGGAAYRLTTHAGQEVFPKISPDGKWIAFSGEYSGTRQVHVI
                     PSWGGEPKQLTFYNDAGPMPPRGGFDNWVMGWTKDGKILVRMNRVPWNSRMGRYFTVD
                     PKGGLETPLEIPEGGSASFSPDGTKLAYCPVDREFRTWKRTLGGRAQDVYVYDFASKA
                     SERITDWKGTDNFPMWNGDTIWFTSDRDHTLNLFAYDVKTKKTRKATSFTDFDVLWPS
                     LASDRSAIVFMNGGYVWRLDPKTEKASKIAITLASDRAPLGTAWKDVKEFVRGASLSP
                     SGARVILEARGDLFSVPSKDGQTRTLTDTQGVRERAPAWSPDGKKVAYLSDATGEYEI
                     WVRAADGSGEPKPLTKDGAPWKFDPAWSPDGKKLAYGDRKHRLRVVDVESGAIADVDT
                     GAREDLDTYTWSPDSKWLAYEKSHPTRLPGIAVWSADSKKAFPLGDGLTRDHNPAFSA
                     DGKYLFFLSERDYPLVFSAFEFNYVYPRATRVYVAALAAGTPSLFPPKNDEEKGTDAK
                     EKADKGDKPEKDTDRKKDEKPEKKDAAPTVVDADGFVLRTQALPGVPAGNYPEMAATT
                     DAVWYVRGGDGDGGAAGRALYRFDLKERKEEKALDGVAGWELSRDGKKILTRVGSDWF
                     VTDAKAPLKGGDGKLDLSGLRVKLDVAAETRQMWDDAWRIVRDWFYDEKMHGTDWAGL
                     KKRYGALVPYVAHRADLDFLFGEIMGELEAGHTYVANGDEPKVPRVAGGMLGCEFAAD
                     ASGRYRIAKVFAGENWDDGWRSPLTEPGVGVKEGSFLLAIDGRDLTTADNPYRLLEGK
                     GGKLVSLLVSDTAAREGARTVQVRTITSEQNLRYLDWLKSRMAMVDRLSGGRVGYVHL
                     PDTAQAGNRMLQKLFYSQAGKPALLIDDRYNGGGFIPDRMIEVFSRKTLAFWARRGLE
                     SNRAPGFAHDGPKAMLVNSYSSSGGDALPYFFRVNGLGPVIGTRTWGGLIGLSGGPSL
                     VDGGAVQVPSFRIYDREGKWVVENEGVAPDIEVFDDAGSRVKGGDPALETGVAVLLKE
                     LEKPGAWKEPKAPVPPDMAAQKP"
BASE COUNT          572 a         1187 c         1225 g          481 t
ORIGIN      
        1 cccggtcgta ttctccaatc gatgaagaac cgcctgagcc tctttgccct cctgctgccc
       61 ttgtgccttc ttctcgcctc cggccctgtc ctcgccgccg gccccgacgg cccgcccgag
      121 gcgaggctcc tgcgttaccc ggacgcctgg ggcgacttcg tcgtcttcac atacgcgggc
      181 gacctctggc gagcgcctct cgcaggcggc gccgcgtacc gcctcacgac gcacgcgggc
      241 caggaggtct tcccgaagat ctcgcccgac gggaaatgga tcgcgttctc gggcgagtac
      301 tcggggacgc ggcaggtcca cgtcatcccg tcctggggcg gcgagccgaa gcagctcacg
      361 ttctacaacg acgcggggcc gatgccgccg cgcgggggct tcgacaactg ggtgatgggc
      421 tggacgaagg acgggaagat cctcgtccgc atgaaccgcg ttccgtggaa cagccgcatg
      481 gggcggtact tcacggtcga cccgaagggc ggcctcgaga cgcctctcga gatcccggag
      541 ggcggctcgg cctccttctc gccggacggg acgaagctcg cgtactgccc tgtggaccgc
      601 gagttccgga cctggaaacg gacgctcggc gggcgcgcgc aggacgtcta cgtctacgac
      661 ttcgcgtcga aggcctcgga gcgcatcacg gactggaagg gcaccgacaa cttcccgatg
      721 tggaacgggg acacgatctg gttcacgtcc gaccgcgacc acacgctcaa cctcttcgcc
      781 tacgacgtga agacgaagaa gacgcggaag gcgacgtcgt tcacggactt cgacgtgctg
      841 tggccgtcgc tcgcctcgga ccggagcgcg atcgtcttca tgaacggcgg gtacgtctgg
      901 cgcctcgacc cgaagacgga gaaggcctcg aagatcgcga tcacgctcgc gtcggaccgc
      961 gccccgctcg ggacggcgtg gaaggacgtc aaggagttcg tccgcggcgc gagcctctcg
     1021 ccgagcggcg cgcgcgtgat cctcgaggcg cgcggcgacc tcttcagcgt gccttcgaag
     1081 gacggccaga cgcgtaccct cacggacacg cagggcgtgc gcgagcgcgc gcccgcgtgg
     1141 tcgcccgacg ggaagaaggt cgcgtacctc tcggacgcga cgggtgaata cgagatctgg
     1201 gtgcgcgcgg ccgacgggtc cggcgagccg aagccgctca cgaaggacgg cgcgccgtgg
     1261 aagttcgacc cggcgtggag cccggacggg aagaagctcg cgtacggcga ccggaagcac
     1321 cgcctgcgcg tcgtcgacgt ggagtcgggc gcgatcgccg acgtggacac gggagcgcgg
     1381 gaggacctcg acacgtacac gtggtcgccc gactcgaagt ggctcgcgta cgagaaaagc
     1441 catccgacgc gcctcccggg gatcgccgtg tggtccgccg actcgaagaa ggcgttcccg
     1501 ctcggcgacg ggctgacgcg cgaccacaac cccgcgttct ccgccgacgg gaaatacctc
     1561 ttcttcctct ccgagcgcga ctacccgctc gtcttctcgg cgttcgagtt caactacgtg
     1621 tacccccgcg cgacgcgcgt ctacgtcgcc gcgctcgcgg cgggcacgcc ctccctcttc
     1681 ccgccgaaga acgacgagga aaagggcacg gacgcaaagg aaaaggccga caagggcgac
     1741 aagcccgaga aggacaccga caggaagaaa gacgaaaagc ccgagaagaa ggacgcggct
     1801 ccgaccgtgg tcgacgccga cggattcgtc ctccgcacgc aggcgctgcc gggcgtcccg
     1861 gcgggcaact atccggagat ggccgcgacg accgacgccg tctggtacgt ccgcggggga
     1921 gacggcgacg gcggtgcggc cgggcgcgcg ctgtaccgct tcgacctcaa ggagcgcaag
     1981 gaagagaagg ctctcgacgg cgtcgccggc tgggagctct cgcgcgacgg caagaagatc
     2041 ctcacccgcg tcggatccga ctggttcgtc acggacgcca aggcgccgct caagggcggg
     2101 gacggcaagc tcgacctctc cggcctccgg gtgaagctcg acgtcgccgc cgagacgcgg
     2161 cagatgtggg acgacgcctg gcggatcgtc cgcgactggt tctacgacga aaagatgcac
     2221 ggcacggact gggccgggct gaagaagcgt tacggcgcgc tcgtcccgta cgttgcgcac
     2281 cgcgccgacc tcgacttcct gttcggcgag atcatgggcg agctcgaggc ggggcacacg
     2341 tacgtcgcga acggcgacga gcccaaggtg ccgcgcgtcg cgggcgggat gctcggctgc
     2401 gagttcgcgg ccgacgcctc cggccgctac cgcatcgcga aggtcttcgc gggcgagaac
     2461 tgggacgacg gctggcgctc gccgctcacg gagccgggcg tgggcgtgaa ggagggcagc
     2521 ttcctcctcg cgatcgacgg ccgcgacctg acgacggccg acaatccgta ccgtctcctc
     2581 gagggcaagg gagggaagct cgtctccctt ctcgtctcgg acacggccgc gcgggagggc
     2641 gcccgcaccg tccaggtccg gacgatcacg tccgagcaga acctgcgcta tctcgactgg
     2701 ctgaagagcc gcatggcgat ggtcgaccgg ctctccggcg gcagggtcgg ctacgtccat
     2761 cttcccgaca cggcgcaggc gggcaaccgg atgctccaaa agctcttcta ctcgcaggcg
     2821 ggcaagcccg cgctcctcat cgacgaccgc tacaacggag gcgggttcat cccggaccgg
     2881 atgatcgagg tcttctcgcg caagacgctc gcgttctggg cgcgccgcgg cctcgagagc
     2941 aaccgcgcgc cgggcttcgc gcacgacggt cccaaggcca tgctcgtcaa ctcctactcg
     3001 tcctcgggcg gcgacgcgct gccgtacttc ttccgggtga acggcctcgg acccgtcatc
     3061 gggacgcgca catggggcgg gctcatcgga ctctccgggg gcccgtcgct cgtggacggc
     3121 ggggccgtgc aggtcccgtc gttccggatc tacgaccgcg aggggaagtg ggtcgtcgag
     3181 aacgagggcg tcgcgcccga catcgaggtc ttcgacgacg cgggctcgcg cgtgaaaggc
     3241 ggcgatccgg cgctcgagac gggcgtcgcg gtcctcctca aggagctcga gaagccgggc
     3301 gcatggaagg agccgaaggc tcccgttcct cccgacatgg cggcgcagaa gccgtagggt
     3361 tcgagcgtca gggttcctcg tcgtaggcgg ggaaccactg gcgcgcgacg actccggcgc
     3421 gaagcgtctg ggcggcggcg gccggcgcga ggccctcgcg cacgg
//
LOCUS       DAOMFY010000353         3454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11000_length_3454_cov_7.310385, whole genome shotgun sequence.
ACCESSION   DAOMFY010000353 DAOMFY010000000
VERSION     DAOMFY010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11000_length_3454_cov_7.310385"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLB01_18405"
     CDS             <1..707
                     /locus_tag="PLB01_18405"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQQ79327.1"
                     /translation="FLKLFAAQSAGAFDLAASATVSTGRAGGLSAIGRAAALVRSGRA
                     PFAVAGGVDSFVDLYTLATLDLEKRVKSGAHLDGFIPGEGAAFLLLASPRAAAAAGLR
                     ALAALSEPAEGFEKGHLGSKETYKGEGLAATVQALVGAAPPPRPFASVWSSMNGESHW
                     AKEWGVAFLRSRDAFEDAHAMNHPADCYGDTGAAAGVLLAGLASLDAPNGPALAYASS
                     DFGPRSAISILPAGGT"
     gene            707..1090
                     /locus_tag="PLB01_18410"
     CDS             707..1090
                     /locus_tag="PLB01_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HQQ79328.1"
                     /translation="MAATFANFRGIVHKQSGGTSIVFPDVCKTPAPPAPSPVPIPYPN
                     VGMSSDTSQGPNTVTTDGAMPMVKGAQYMRSSGDEAGVAGGILSSINMSVCEYLLYSF
                     DVKFEGNNVCRMGDQLFHNKKNIMG"
     gene            1179..1955
                     /locus_tag="PLB01_18415"
     CDS             1179..1955
                     /locus_tag="PLB01_18415"
                     /inference="COORDINATES: protein motif:HMM:NF025769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HQQ79329.1"
                     /translation="MPKSKKNVLKQNFDEDSLIGLVPGGIEHENNSGTLGTNCASTHP
                     SPLRVQFDDWEEDLPVEFAAHHLIPGDASLQKSKLFRDADYGLQIEGEDEGNVGYDVN
                     NTTNGVWLTGNYAYSQRGKKRGKRLKSQWGEDGETFERVTGIPAQEYVDAAIEFSGAQ
                     FHDAHTDYNEIVRKKLDQIYDKLEKFQDVPWCPEAAKTKKAKKEPLYTLVNRLASLSL
                     NLRSRLTFPTKHWKYDLYTSGFSESWMKNQDRNRTRRRSL"
     gene            1966..2544
                     /locus_tag="PLB01_18420"
     CDS             1966..2544
                     /locus_tag="PLB01_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79330.1"
                     /translation="MYFVLRRSSAKHPCRWIQDWPFIKGVGWSFGTRIERDLPKPLRF
                     TLERLKSNAGDQGPEMPEWLQGCEIPLFRDDLVEALRASGVDNIDFYETLVEDPDSGK
                     TYSNYKAANIIGLVAAADMSRSEATVHPGGPVVDVDFEHLVLDEARARGALIFRLAES
                     TGAILVHEQLRDRLLAAGFHTLDFIEPEHSIA"
     gene            2615..2986
                     /locus_tag="PLB01_18425"
     CDS             2615..2986
                     /locus_tag="PLB01_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79331.1"
                     /translation="MLAVDEHGAVSVTFSDEESAFPADLLVTSDSLALRVAPGDDVLV
                     WTPPDGSRAVVVGRLEAGNGAPGRVPDELLLEARKGLVLRVGEGSITIRADGKILIKG
                     KDLVSHAKRMNRIKGGSVSLN"
     gene            3006..>3454
                     /locus_tag="PLB01_18430"
     CDS             3006..>3454
                     /locus_tag="PLB01_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79332.1"
                     /translation="MTDAPLKFLPDLVEEHFEELAFLWGQRRGALRSPRFMPRDLGRL
                     EERIEAHVSGLLIAGDELSEFVRPGLEGEDADEAFAAGFGLLRSKVAGGADRVWEAFE
                     SATGPRADGLAEAFAHGPSEALLSRLQGAVTSAPPPRSVAAAEALALR"
BASE COUNT          619 a         1138 c         1137 g          560 t
ORIGIN      
        1 ggtttctcaa gctcttcgct gcgcagtccg ccggcgcctt cgacctcgcc gcgagtgcga
       61 ccgtgagcac cggccgcgcc ggcggcctct cggcgatcgg gcgcgccgcg gccctcgtgc
      121 gctcgggaag ggcgccgttc gccgtggcgg gcggcgtcga ctcgttcgtc gacctctaca
      181 cgctcgcgac gctcgatctc gagaagcgcg ttaagtcggg cgcgcacctc gacggcttca
      241 tccccggcga aggcgcggcg ttccttctcc tcgcgagccc gcgcgccgcg gctgccgcag
      301 gcctccgggc gctcgccgcc ctgtcggagc cggccgaagg cttcgagaag ggccacctcg
      361 ggagcaagga gacctacaag ggagaggggc tcgccgcgac ggtccaggct ctcgtcggcg
      421 ccgctccgcc accgagaccc ttcgcgtcgg tgtggtcgtc gatgaacggc gagagccact
      481 gggccaagga gtggggcgtc gcgttcctgc gctcgcgcga cgccttcgag gatgcgcacg
      541 cgatgaacca tccggccgac tgctacggcg acaccggagc ggcagcgggg gttcttctcg
      601 cggggctcgc gagcctcgac gccccgaacg gacctgcgct cgcctacgcc tcgtccgact
      661 tcggacctcg gtccgccatt tcgatcctgc cggcgggagg aacctgatgg ccgcaacgtt
      721 cgcgaacttc cgcggcatcg ttcacaagca gagcggcggg acgtccatcg tgtttccgga
      781 cgtctgcaag acgccggcgc cgccggcgcc gagcccggtg ccgatcccct acccgaacgt
      841 cgggatgtcc tcggacacct cgcaggggcc caacaccgtc acgacggacg gcgcgatgcc
      901 gatggtcaag ggtgcgcagt acatgcgttc ctcgggcgac gaggccggcg tcgccggcgg
      961 catcctctcg agcatcaaca tgagcgtctg cgagtacctg ctctattcct tcgacgtgaa
     1021 gttcgagggc aacaacgtct gccggatggg agaccagctc ttccacaaca aaaagaacat
     1081 catggggtga cggtgtgacg gacttcggtg agagcgcaag cgtggacgtt cttctcgata
     1141 accacgacga agactgctac ttctgcacga agaagtccgt gccgaagtcg aagaagaacg
     1201 ttttgaaaca gaacttcgac gaagattcgc tcatcggcct ggttcccgga ggcatcgagc
     1261 acgaaaacaa ctccggcacg ctcggaacga actgcgcgtc gacacatccc agccctctcc
     1321 gcgttcagtt cgacgactgg gaggaggatc tcccggtcga gttcgccgcg caccacctca
     1381 ttcccggcga cgcctccctg cagaagagca agctctttcg cgatgcggac tacggactgc
     1441 agatcgaggg agaggacgaa ggaaacgtcg ggtatgacgt caacaacacg acgaacggcg
     1501 tctggttgac cggcaactac gcctacagcc agcgggggaa gaagagggga aagaggctca
     1561 agagtcagtg gggcgaggac ggggagacgt tcgagagggt cacgggcatc ccggcccagg
     1621 agtatgtcga cgctgcgatc gagttctcgg gcgcgcagtt tcacgacgcc cacaccgact
     1681 acaacgaaat cgtgcggaag aaactcgacc agatttacga caagctcgag aaattccagg
     1741 atgtgccgtg gtgcccggaa gcagcaaaga cgaagaaggc caagaaggag cctctttaca
     1801 cgcttgtgaa ccgtctggcc agcctctcgc tgaacctccg aagccgactg acgttcccca
     1861 caaagcactg gaaatacgac ctctacacgt ccgggttttc ggagtcctgg atgaagaatc
     1921 aggaccgtaa ccgtacccgg cgccgaagcc tgtaagggcg tcgccatgta cttcgtcctc
     1981 agacgctcct cagccaagca tccgtgccgg tggatccagg actggccctt catcaaaggg
     2041 gtcggttggt ccttcggaac gcggatcgag cgcgacctgc cgaaaccgct gcgattcacg
     2101 ctcgagcggc tcaagtcgaa cgccggcgat cagggacccg agatgcccga gtggctgcag
     2161 ggctgcgaga ttcctctgtt tcgcgatgat ctcgtggagg ccctgcgggc gtccggagtc
     2221 gacaacatcg atttctacga gactctcgtc gaggacccgg acagcggaaa gacgtacagc
     2281 aactacaagg cggcgaacat catcgggctg gttgccgccg cggacatgag tcgatcggaa
     2341 gcgacggttc atcccggggg cccggtggtc gacgtcgact tcgagcacct cgtcctcgac
     2401 gaggcaagag ctcgcggcgc actcatcttc cgtctcgccg agtcgaccgg cgcgattctc
     2461 gttcacgagc aacttcgcga tcgcctcttg gccgccggct ttcacacgtt ggacttcatc
     2521 gaaccagagc actcgatcgc atgacctcag gagaatgaca ttggacacga tcgtgacaac
     2581 tccgaagagc cagctttcga tgacgcgtgg gaaggtgctc gccgtcgacg agcacggtgc
     2641 tgtgagcgtg acgttctcgg acgaggagag cgccttccct gccgatctcc tcgtcacatc
     2701 ggattcgtta gcgcttcgcg tcgctccagg cgacgacgtc ctcgtgtgga cgccccccga
     2761 tggctcccgg gccgtcgtcg tcggtcgcct ggaggccggg aacggggcgc ctggccgcgt
     2821 acccgatgaa cttcttctcg aagccaggaa gggactcgtc ctccgcgtcg gggagggttc
     2881 catcacgatc cgcgccgacg ggaagatcct catcaagggc aaggacctcg tctctcacgc
     2941 gaagcgcatg aaccgcatca agggcgggtc ggtgtcgctc aattgagaag ggccgcgcgg
     3001 cgaccatgac cgacgctccg ctgaagttcc tgcccgacct cgtggaggag cacttcgagg
     3061 agctcgcgtt cctctggggg cagcggcgcg gggcgctgcg ctcgccgcgg ttcatgccgc
     3121 gcgacctcgg ccggctcgag gagaggatcg aggcgcacgt ctcgggcctc ctgatcgcgg
     3181 gcgacgagct ctcggaattc gtccggcccg ggctggaagg cgaggacgcc gacgaggcct
     3241 tcgccgcggg ctttggcctc ctccgttcga aggtcgcagg cggcgcggac agggtctggg
     3301 aggctttcga gtccgcgacg ggcccgcgcg ccgacggact ggcggaggcg ttcgcccacg
     3361 gcccgtccga ggcgctgctc tcgcgcctgc agggggccgt cacctccgcg ccgccgcccc
     3421 gctccgtcgc cgcggccgag gcgctcgcgc tgcg
//
LOCUS       DAOMFY010000354         3454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11002_length_3454_cov_5.122389, whole genome shotgun sequence.
ACCESSION   DAOMFY010000354 DAOMFY010000000
VERSION     DAOMFY010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11002_length_3454_cov_5.122389"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLB01_18435"
     CDS             complement(<1..437)
                     /locus_tag="PLB01_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79333.1"
                     /translation="MPGARAEATARDWRLNTERALALSIILHLLLVIVLLTVKFPERR
                     TDEPQPDPLGLLSLMQATPPDPAIPVQLFPGPKAAQPGPRPAPSDLNRQAHGGDPKQP
                     VQPMPKAYPQAGIRDMDAGRPGAPAPPAARPPQGTETGEPGKLT"
     gene            794..1267
                     /gene="rimP"
                     /locus_tag="PLB01_18440"
     CDS             794..1267
                     /gene="rimP"
                     /locus_tag="PLB01_18440"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HQQ79334.1"
                     /translation="MTGKSLPIDAGTESLLGERLEALGLELCHADWNPGRGRATLTLF
                     IDRPGGVTLEDCEAASRAASEVLDPLEESLPGYVLEVSSPGLDRPLWTLSDCEKFKGR
                     RVTVRLERKVDGAAKLKGILEDVRGDSLTVLDEDQKRRYTVRFGDVRIARLVPEL"
     gene            1290..3044
                     /gene="nusA"
                     /locus_tag="PLB01_18445"
     CDS             1290..3044
                     /gene="nusA"
                     /locus_tag="PLB01_18445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQQ79335.1"
                     /translation="MSTQPPTENGPAPEKKKTKAPKGAAKPRAKAAKAAAAPPATKAD
                     EIIEALKAAARDKEIEFDRLVAALEEAIATAARKVYKVREMAARFDAASGLLTAWTPY
                     RVVEHKTKPEEPEAEAILDPDDVPLGAAAAPVPAAAVAPVDPDKEIRLPWIEVLPEEA
                     HALLAGELAGQSWQVVRNPKGEESTGDEEIIDAAQIGLGDEIRLYRSTEGLGRIAAQS
                     AKQVLYQKLREAERDNIYNEYFPKVGELITGTVKRFERGDMIVDLGRTEAAIPREHQS
                     RAERYTQGERVRGVLVDVHKNPKGSQIILSRTAPELLMRLMEMEVPEIYDGTVVIRGC
                     VRQPGDRAKVAVSSRERDVDPVGACVGMRGARVQAIMRELRGERIDIIQYSDDLVTYA
                     QNALSPAKITRVSAMPADDEAEPEIGADGEPIEPVPTLECIVEEDQLSLAIGKRGQNV
                     RLAAALIGARIEIKSEQAVKAEVADALQRMLIASQRRGTALSEVPDLDAAAASAFEAA
                     GFATVGDLLDAALGEGENPLAAVALEPAARDAAIEAARAFDEAPPEDDEEEEETLEFQ
                     ETADEESSSAPVTETPEK"
     gene            3041..>3454
                     /locus_tag="PLB01_18450"
     CDS             3041..>3454
                     /locus_tag="PLB01_18450"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HQQ79336.1"
                     /translation="MKPTAAKFTVTQLAKMMGKPPKEVLFLLQGIGVDVKTPDSVLDA
                     ATASALLAGKTQAPKTLIVRQAPTAPSAAALATAAAAKAKTERAALKRIKIVEKTAEG
                     EAETAEAGEFTEAAAQAPAAAEAAAPASTRADAGAE"
BASE COUNT          571 a         1241 c         1211 g          431 t
ORIGIN      
        1 gtcagcttgc cgggctcccc cgtctcggtc ccctgcggcg ggcgggccgc aggaggcgcc
       61 ggcgcgcccg gccgtccggc atccatgtct cgaatgccgg cctgcggata cgccttcggc
      121 atgggctgca ccggctgttt cgggtcgccc ccgtgcgcct ggcggttcaa gtccgagggg
      181 gccgggcgcg gcccgggctg cgcggccttc gggccgggga agagctggac ggggatcgcc
      241 gggtccggcg gcgtcgcctg catgagggag aggaggccga gcgggtcggg ctgcggctcg
      301 tccgtgcgcc gctcggggaa cttgaccgtg agcaggacga tgacgaggag gagatgcagg
      361 atgatcgaga gcgcgagcgc gcgctcggtg ttgagccgcc agtcgcgggc cgtcgcctcg
      421 gcgcgcgcac cgggcatgaa ctcgggtgtc tcggcaagag ccattgccga gaaggggtcc
      481 tcctcgggcg cgagtccccc cgggggaggc cacggggcaa gctcttcctg cccttcgacg
      541 gcttcgtcgt tcagacgaat gacgatgaga tcgctgatgg agtttcccct cgagagggcg
      601 ctcggttgtc gcgcggcccg ttcctatggg aagtccatgc cacttcccgc cggcatgtta
      661 gcggctttcg cgccttgccc gcggagaccc gcgagcctat aatgagagag cagctttcaa
      721 ggggcttgaa agagggcata ggccctcttt tttttgcccc atgcgggctg cgggaagagc
      781 ccaagacgaa tcgatgaccg gaaagagcct gccgatcgac gcggggacgg agagcctcct
      841 cggtgagcgc ctcgaagcgc tcgggctgga gctgtgtcat gccgactgga acccgggccg
      901 cgggcgcgcc acgctgacgc tcttcatcga ccgtccgggc ggcgtcacgc tcgaagactg
      961 cgaggccgcg tcccgcgcgg cgtcggaagt cctcgacccg ctcgaggaga gcctgcccgg
     1021 gtacgtcctc gaggtctcct cccccggcct cgaccgcccg ctctggacgc tttcggactg
     1081 cgagaagttc aagggccgcc gcgtgaccgt gcgcctcgag cgcaaggtcg acggggccgc
     1141 gaaactcaag gggatcctcg aggacgtccg cggggactcc ctgacggtcc ttgatgagga
     1201 tcagaagcga cgctacactg tgcgattcgg cgatgtccgg atcgcgcggc ttgtccccga
     1261 actgtaaacc cgacggagaa cacgacacga tgagcaccca gcccccgacg gagaacggcc
     1321 cggcgcccga aaagaagaag acgaaggcgc cgaagggcgc ggcgaagccg cgcgcaaagg
     1381 ccgccaaggc ggcggccgcg ccgccggcca cgaaggccga cgagatcatc gaagccctca
     1441 aggccgccgc gcgcgacaag gagatcgagt tcgaccgtct cgtcgccgcg ctggaagagg
     1501 cgatcgcgac ggctgcccgc aaggtctaca aggtgaggga gatggccgcc cgcttcgacg
     1561 cggcgagcgg cctcctgacg gcgtggaccc cgtaccgggt cgtcgagcac aagacgaagc
     1621 ccgaggagcc cgaggccgag gccattctcg atccggacga cgtcccgctc ggcgccgctg
     1681 ccgcgcccgt cccggccgcg gccgtcgcgc ccgtcgaccc cgacaaggag atccgcctcc
     1741 cctggatcga ggtccttccc gaggaagcgc acgcgctcct cgcgggcgag ctggcgggcc
     1801 agagctggca ggtcgtccgc aacccgaagg gcgaggagtc cacgggcgac gaggagatca
     1861 tcgacgccgc ccagatcgga ctcggcgacg agatccgcct ctaccgctcg accgagggcc
     1921 tcggccgcat cgcggcccag agcgccaagc aggtgctcta ccagaagctt cgcgaggccg
     1981 agcgcgacaa catctacaac gaatacttcc cgaaggtcgg ggagctcatc acgggcaccg
     2041 tgaagcggtt cgagcgcggc gacatgatcg tcgacctcgg gcgcaccgag gccgcgatcc
     2101 cgcgcgagca ccagtcgcgc gccgagcgct acacgcaggg cgagcgcgtc cgcggcgtcc
     2161 tcgtggacgt ccacaagaac ccgaagggct cgcagatcat cctgtcgcgc acggcgcccg
     2221 agctcctcat gcgcctcatg gagatggaag tccccgagat ctacgacggc accgtcgtga
     2281 tccgcggctg cgtccgccag cccggcgacc gcgcgaaggt cgcggtctcg tcgcgcgagc
     2341 gcgacgtgga cccggtcggc gcgtgcgtcg gcatgcgcgg cgcgcgcgtg caggcgatca
     2401 tgcgcgagct gcgcggcgaa cgcatcgaca tcatccagta ctcggacgac ctcgtcacgt
     2461 acgcgcagaa cgcgctctcc cccgcgaaga tcacgcgcgt ttcggccatg ccggccgacg
     2521 acgaggccga gccggagatc ggcgccgacg gcgagcccat cgagcccgtc ccgacgctcg
     2581 agtgcatcgt cgaggaggac cagctctccc tcgcgatcgg caagcgtggg cagaacgtcc
     2641 gcctcgcggc cgccctcatc ggcgcccgca tcgagatcaa gagcgaacag gccgtcaagg
     2701 ccgaggtcgc cgacgcgctc cagcgcatgc tcatcgcgtc gcagcgccgc ggcacggctc
     2761 tgtccgaggt gcccgacctc gacgcggccg ccgcgtcggc gttcgaggcc gccgggttcg
     2821 ccaccgtggg cgacctcctc gacgccgccc tcggcgaagg cgagaacccg ctcgcggccg
     2881 tggcgctcga gcccgccgcc cgcgacgccg cgatcgaggc tgcgcgcgcc ttcgacgaag
     2941 cgccccccga ggacgacgag gaagaagaag agacgctcga gttccaggaa accgccgacg
     3001 aggagtcctc gtccgctccc gtgacggaga ctcccgaaaa gtgaaaccga ccgccgcgaa
     3061 gttcaccgtc acccagctcg cgaagatgat gggcaagccc ccgaaggagg tcctcttcct
     3121 cctccagggc atcggcgtcg acgtcaagac ccccgattcc gttctcgacg ccgcgaccgc
     3181 ctcggccctc ctggcgggca agacacaggc gccgaagacg ctcatcgtcc ggcaggcgcc
     3241 caccgccccg tccgccgccg cgctcgcgac ggccgccgcc gccaaggcca agaccgagcg
     3301 cgcggcgctc aagcgcatca agatcgtcga gaagaccgcc gagggcgagg ccgaaacggc
     3361 cgaggcgggc gagttcaccg aggccgcggc ccaggctccg gcagctgccg aagccgccgc
     3421 ccccgcctcg acccgtgccg acgcgggcgc cgaa
//
LOCUS       DAOMFY010000355         3441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11095_length_3441_cov_7.585056, whole genome shotgun sequence.
ACCESSION   DAOMFY010000355 DAOMFY010000000
VERSION     DAOMFY010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11095_length_3441_cov_7.585056"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLB01_18455"
     CDS             <1..326
                     /locus_tag="PLB01_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79337.1"
                     /translation="KEGDATAALGTAAKRIKATYDAPFLAHATMEPMGAVADVRPDRY
                     EASLKAYADLEISWLLSGHLPPVAAPAASRVIARAASFPLEGRVPAPGQQALAAALAH
                     AAAAA"
     gene            367..975
                     /locus_tag="PLB01_18460"
     CDS             367..975
                     /locus_tag="PLB01_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HQQ79338.1"
                     /translation="MNRTTSRSVIAVAAGAGLASAVAILFAAPAAEAARKDAQTTRVE
                     RGRYLVTAMMCNDCHTPWHVGPKGPEPDMTKMLSGHPQGLKMPPAPAAKGPWIVSMAA
                     TNTAFAGPWGVSYAANLTPDQNTGLGIWREEDFVKAIRTGKHFGVSREILPPMPWPFI
                     RTLTDEDLKSMYAYLRTIPPVSNLVPDPDPPAAVPPSIASKK"
     gene            1067..2080
                     /gene="gap"
                     /locus_tag="PLB01_18465"
     CDS             1067..2080
                     /gene="gap"
                     /locus_tag="PLB01_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQQ79339.1"
                     /translation="MTTPVRVGINGFGRIGRCLFKHLLQRGATVAGINDLADLGDLAY
                     LLKYDSVHGWWDHRVSVTDHELVVDGMKIPFFSSKDPSTIPWGASGADVVVECSGAFR
                     KRADAAKHLAGGAKKVVISAPADDPDGTFVLGVNAHTYDPGKHHVVSMASCTTNSLAP
                     VAKVLNDSFGVEHLMITTVHAYTSSQSLMDAPTRKRRRGRAAALSIVPTSTGAAKATA
                     LVIPELKGKMDGMALRVPIPDGSITDIVVTLKKDVTPDAINAALATAAETPALKGILR
                     VADEALVSRDILGDTHSAIVDKDSTMVLDKRVAKVLVWYDNEWGYAARLADFAALLAT
                     KGF"
     gene            2138..3052
                     /locus_tag="PLB01_18470"
     CDS             2138..3052
                     /locus_tag="PLB01_18470"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ79340.1"
                     /translation="MKAFARVAPLALILAAGACGRAVSVTHLVPAPYNLGPAKRLVLV
                     EVSGDFLSRSDAASIFMTEVARGGVFEARDATRAWTSLSDLGAGAAARKAKEFRLAWP
                     ADVYVGLDVAEFETSDRREKTTEKREGQDVEVVRYFAVADCRIRVRILDAKDGRVLAD
                     YEVARRRRSYKSAHPEAGQPVDAKKRALEAAVVESVAGFTPSRVDEWVLLDDEAPLAK
                     EGIALYDKKDLAGARLLWEKGLATYPKDARLRYNLGAVSEALGDPRAAREYYEDAIAL
                     APGDARYQRALDTLDDRMRDAEALRTKP"
     gene            3049..>3441
                     /locus_tag="PLB01_18475"
     CDS             3049..>3441
                     /locus_tag="PLB01_18475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ79341.1"
                     /translation="MKPARAALAAAFAIGVLARLGAALARPLWADEIFTLTVARRSVP
                     EILAALRVDSGPPLHYLAAHVLLLPFPAPGAGDVVVRLLSLVSSLLHLPLLFFVGKRL
                     GRPTAGLAAAALYAVFPLAVLYGVEGRGY"
BASE COUNT          509 a         1315 c         1133 g          484 t
ORIGIN      
        1 gcaaagaggg cgacgccacc gcggcgctcg gcacggcggc caagcggatc aaggccacct
       61 acgacgcgcc cttcctggcc cacgccacca tggagcccat gggggccgtg gcggacgtgc
      121 ggcccgaccg ctacgaggcg tccctgaagg cgtacgcgga cctcgagatc tcgtggctcc
      181 tgagcggcca cctgccgccc gtcgcggcgc ccgccgcgag ccgcgtgatc gcccgggccg
      241 cctcgttccc gctcgaggga cgcgttcccg cccccggcca gcaggcgctc gccgccgcgc
      301 tcgcccacgc cgccgccgct gcctgaacga acgaaacgcc acgcgaattc gaaaggaagg
      361 aagaccatga accgcacgac ctcccgctcc gtaatcgccg ttgcggcggg cgccggcctc
      421 gcctccgccg tcgccatcct gttcgccgct cccgccgccg aggccgcccg caaggacgcg
      481 cagacgacgc gcgtcgagcg cggccgctac ctcgtcaccg cgatgatgtg caacgactgc
      541 catacgccgt ggcacgtcgg cccgaagggg cccgagccgg acatgacgaa aatgctctcg
      601 ggccacccgc aaggcctgaa gatgcctccc gcgcccgccg cgaaggggcc gtggatcgtg
      661 tcgatggccg cgacgaacac cgcgttcgcc ggtccctggg gcgtcagcta cgccgcgaac
      721 ctcacgccgg accagaacac gggcctcggg atctggcgcg aagaggactt cgtgaaggca
      781 atccggaccg gcaaacactt cggggtgtcg cgcgagatcc tgccgccgat gccctggccg
      841 ttcatccgca cgctcacgga cgaggacctg aagtcgatgt acgcctacct ccggactatt
      901 ccgcccgtct cgaacctcgt ccccgacccc gacccgcccg ccgccgtgcc cccgtcgatc
      961 gcatcgaaga agtgacggcc tgatgcaccc gcttcaggcc ggtcctcggt ccggccctta
     1021 agatctctcc ggggcgcgtg cggacgcacg gccccggagg ttcttcatga cgactccggt
     1081 tcgtgtcgga atcaacggct tcggccgcat cggccgctgc ctcttcaagc acctgctcca
     1141 gcgcggcgcc accgttgccg gaatcaacga cctcgcggat ctcggggacc tcgcctatct
     1201 cctcaagtac gactcggtcc acggctggtg ggaccaccgc gtctcggtga cggaccacga
     1261 gctcgtcgtg gacggcatga agattccctt cttctcctcg aaggacccct cgacgattcc
     1321 ctggggcgcc tccggcgccg acgtcgtcgt cgagtgctcc ggcgcgttcc gcaagcgggc
     1381 ggacgccgcc aagcacctcg cgggcggcgc gaagaaggtc gtgatctcgg cgccggccga
     1441 cgacccggac ggcacgttcg tcctcggcgt gaacgctcac acgtacgacc cgggcaagca
     1501 ccacgtcgtt tcgatggcct cctgcacgac gaactcgctc gcgcccgtcg cgaaggtcct
     1561 gaacgactcg ttcggcgtcg agcacctcat gatcacgacg gtccacgcct acacgtccag
     1621 ccagtccctg atggacgccc ccacgcgcaa gcgccggcgg ggccgtgcgg ccgccctctc
     1681 catcgtgccg acgtcgacgg gcgccgcgaa ggcgaccgcc ctcgtcatcc ccgagctgaa
     1741 ggggaagatg gacgggatgg cgctccgcgt gccgattccc gacggctcga tcacggacat
     1801 cgtcgtgacg ctcaagaagg acgtcacgcc ggacgcgatc aacgcggcgc tcgcgaccgc
     1861 ggccgagacg cccgcgctga aaggcatcct ccgcgtcgcc gacgaggcgc tcgtgtcccg
     1921 cgacatcctc ggcgacacgc actcggcgat cgtcgacaag gactcgacga tggtgctcga
     1981 caagcgcgtc gccaaggtcc tcgtgtggta cgacaacgag tggggctacg ccgcgcgcct
     2041 cgccgacttc gccgcgctcc tcgccacgaa gggcttctga tcggccgtcc tcccgggtgc
     2101 tagtctttcc cgtccgatcc gggaaaggag cacccctgtg aaggcgttcg cccgcgtcgc
     2161 gcccctcgcc ctgatcctcg cggcgggcgc gtgcggccgc gccgtgtccg tgacgcacct
     2221 cgtgcccgcg ccctacaact tggggccggc gaagcgcctc gtcctcgtcg aagtgagcgg
     2281 cgatttcctg tcgcggtcgg acgcggcttc catcttcatg accgaggtcg cccgaggcgg
     2341 cgtcttcgag gcccgggacg ccacgcgggc atggacgtct ctctcggacc tgggggcggg
     2401 cgccgccgcg cgcaaggcga aggagttccg actcgcctgg ccggccgacg tctacgtcgg
     2461 gctcgacgtc gccgagttcg agacgtccga ccgccgcgag aagacgacgg agaaacgcga
     2521 gggacaggac gtcgaggtcg tccgctactt cgcggtcgcg gactgccgga tccgcgtgcg
     2581 catcctcgac gcgaaggacg ggcgcgttct cgcggactac gaggtcgcgc gccggcgccg
     2641 gtcgtacaag agcgcgcatc ccgaggccgg acagcccgtc gacgcgaaga agcgcgcgct
     2701 cgaggccgcg gtcgtcgaga gcgtcgcggg attcacaccg tcgcgcgtgg acgagtgggt
     2761 gctgctggac gacgaggccc cgctcgcaaa ggaaggcatc gcgctctacg acaagaagga
     2821 cctcgccgga gcccggctcc tctgggaaaa aggcctcgcg acgtacccga aggacgcgcg
     2881 cctgcgctac aacctcgggg cggtgtccga agcgctcggc gacccgcgcg cggcgcgcga
     2941 gtactacgaa gacgccatcg ccctcgcacc gggcgacgcc cgctaccaga gagccctcga
     3001 cacgctcgac gaccgcatgc gcgacgccga ggccctgcgg acgaagccgt gaagccggcg
     3061 cgggccgcac tcgccgcggc gtttgcgatc ggcgtcctcg cgcgcctcgg ggccgcgctc
     3121 gcacggcccc tctgggccga cgaaatcttc acgctgacgg tcgcgcgccg gagcgtgccg
     3181 gagatccttg cggccctgcg ggtcgactcc ggaccgccgc ttcactacct cgccgcccac
     3241 gttctcctcc tgcccttccc ggcgccgggc gcgggcgacg tcgtcgtgag gctcctctcg
     3301 ctcgtctcct cgcttctcca cctcccgctg ctcttcttcg tgggcaaacg cctcggccgc
     3361 ccgacggcgg ggctcgccgc ggcggccctc tacgcggtct tcccgctcgc ggttctctac
     3421 ggcgtcgagg gccgcggcta c
//
LOCUS       DAOMFY010000356         3433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11138_length_3433_cov_7.197750, whole genome shotgun sequence.
ACCESSION   DAOMFY010000356 DAOMFY010000000
VERSION     DAOMFY010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11138_length_3433_cov_7.197750"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /gene="accB"
                     /locus_tag="PLB01_18480"
     CDS             <1..323
                     /gene="accB"
                     /locus_tag="PLB01_18480"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQQ79342.1"
                     /translation="PPAAPAPAPPPVAHVAPGPPGSGETEGFHVLTSPIVGTFYRAAN
                     PDAEPFVKVGDVVEKGKTLCIVEAMKLMNEIEADVAGTIVAIYPQNAQPVEFGEKLFA
                     IQPR"
     gene            330..1703
                     /gene="accC"
                     /locus_tag="PLB01_18485"
     CDS             330..1703
                     /gene="accC"
                     /locus_tag="PLB01_18485"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQQ79343.1"
                     /translation="MFQKVLVANRGEIALRIIAACKELGVATVAVHSEADRDALHVRA
                     ADESVCIGPPAPSGSYLNITSLVAAAEITGADAVHPGYGFLAENAHFAEILEEVGLKW
                     IGASPDSIRRMGDKSVAKSTVKESGVPTVPGSPGPLDSIEDSLRLAERIGYPVLLKAV
                     AGGGGRGMRVARNDKELAAAFELATREAERAFGNGSVYLEKYLAEPRHVEIQIFGDTH
                     GRVVHLGERECSLQRRHQKIMEESPSPALSDALRAKMGAAAVAAARAVNYVGAGTVEF
                     LLDEDGSFFFMEMNTRIQVEHPVTEMVTGFDLVKEQIRVAAGEPLSFGATPGDGDGAG
                     WKPRGHAIEFRINAEDPVTFAPSPGTITTFHLPGGPGVRVDTAAYSGWKIPPYYDSLI
                     AKLIVHGRDREEALARGRRALELFIVEGVKTTIPLHLRLLDDPDVRRGRFSTKWLERW
                     LLKHGPG"
     gene            1718..2257
                     /locus_tag="PLB01_18490"
     CDS             1718..2257
                     /locus_tag="PLB01_18490"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQQ79344.1"
                     /translation="MAAAPGRIYLVGFMGSGKTTAGRLLAGTLGVPFVDLDLAFESMA
                     GQTIRETFETRGEPWFRGREAELLRGTASLPSAVIALGGGTFTFPENREFVRAHGLSV
                     FLDVPFAVIAERLAGKTADRPLFPSEERARELWEARLPSYRMADRTLDVTRDMTAADV
                     AGRLADLAAPPLTAPGGAV"
     gene            2259..3017
                     /locus_tag="PLB01_18495"
     CDS             2259..3017
                     /locus_tag="PLB01_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQQ79345.1"
                     /translation="MRYLILSDLHSNDEALTAVLRRVKRKKFERVICLGDFVGYAADP
                     NKVLDRYRTFRRPTYAIRGNHDKVVSGVEEGALFNPPALEAARWTTDRLSRENADFLK
                     KLAPGPAIVDGKFVVCHGSPLDEDAYIFSDFDASLNFVQMHRLAEGVNLCFFGHSHIP
                     SIFTLEPGGIRVEAVRDPRYKLRLDPEKRYLVNPGSVGQPRDRNPRAAYAIYDSDAGV
                     VHFDRVPYDVGTTRRKIERAGLPAVLGDRLLVGL"
     gene            3077..>3433
                     /locus_tag="PLB01_18500"
     CDS             3077..>3433
                     /locus_tag="PLB01_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQQ79346.1"
                     /translation="MSFGGAPGPPKGTYRERLHGFAGLLAMEDRRSYDPLLAGRLAAS
                     PDPWLRAKTALAAGRLRDPDASILLPPLLTDPEASVRRAAAFGSGLSGDARLVPIVAR
                     SLRDADAATAANAAEAL"
BASE COUNT          494 a         1249 c         1173 g          517 t
ORIGIN      
        1 cgccgccggc agcgcccgcg ccggctccgc cccccgtcgc ccatgtcgcg cccggcccac
       61 cgggctcggg cgagaccgag ggtttccacg tcctcacgtc gccgatcgtg ggtacgttct
      121 accgcgcggc caatcccgac gcagagccct tcgtgaaggt cggcgacgtc gtcgagaagg
      181 gcaagacgct ctgtatcgtc gaagcgatga aactcatgaa cgagatcgag gcggacgtgg
      241 cggggacgat cgtggcgatc tacccgcaga acgcgcagcc ggtcgagttc ggcgaaaaac
      301 tcttcgcgat ccagccccgc tgaggcgacg tgttccagaa ggttctcgtc gccaaccgtg
      361 gagagatcgc cctccgcatc atcgccgcct gcaaggagct gggggtcgcg acggtggccg
      421 tccactcgga ggccgaccgt gacgccctcc acgtccgggc cgcggacgaa tcggtctgca
      481 tcgggccgcc cgcgccgtcc ggctcctacc tgaacatcac ctctctcgtc gcggcggccg
      541 agatcacggg cgccgacgcc gtgcatcccg gctacggttt tctcgcggag aacgcccact
      601 tcgccgagat cctcgaggaa gtcggcctga agtggatcgg cgcgtccccc gattcgatcc
      661 ggcgcatggg agacaagtcg gtcgcgaagt cgacggtcaa ggagtcgggc gtgcccacgg
      721 ttcccggaag cccgggcccg ctcgactcca tcgaggactc gttgcggctc gcggaacgga
      781 tcggctaccc ggtgcttctg aaggccgtgg ccggaggcgg cgggcgcggg atgcgggtcg
      841 cgcgcaacga caaggagctc gccgcggcgt tcgagctcgc gacgcgcgag gccgagcgcg
      901 cattcggcaa cggcagcgtc tacctcgaga agtacctcgc cgagccgcgc cacgtcgaga
      961 tccagatttt cggggacacc cacgggcgcg tcgtccacct cggggagcgc gagtgctcgc
     1021 tccagcgccg gcatcagaag atcatggagg agtcgccctc gccggcgctc tccgacgcgc
     1081 tgcgcgcaaa gatgggcgcg gcggccgtcg cggccgcgag ggccgtgaac tacgtcggcg
     1141 ccggcacggt ggagttcctt ctggacgagg acgggtcgtt tttcttcatg gaaatgaaca
     1201 cccgcatcca ggtcgagcac cccgtgaccg agatggtcac gggcttcgac ctcgtgaagg
     1261 aacagatccg tgtcgcggca ggtgagcccc tctcgttcgg cgcgacgccc ggtgacggag
     1321 acggggccgg ctggaagccg cgggggcacg ccatcgagtt ccgcattaac gcggaggacc
     1381 ccgtcacgtt cgctccgtcg cccggaacga tcacgacgtt ccacctcccg ggcgggcccg
     1441 gcgtccgcgt cgacacggcc gcctactcgg gctggaagat cccgccgtac tacgactcgc
     1501 tcatcgcgaa gctcatcgtc cacggccgcg accgcgagga ggcgctcgcc cgtggcaggc
     1561 gcgcgctcga gctcttcatc gtggagggcg tcaagacgac gatcccgctc catctgcggc
     1621 tcctcgacga cccggacgtg cggcgcggac gcttctccac gaagtggctc gagcgctggc
     1681 tcctcaagca cgggccgggc tgaccggggg cccgcgggtg gcggccgcgc cggggcgcat
     1741 ctacctcgtc ggcttcatgg gaagcggcaa gacgaccgcc ggacgcctcc ttgccgggac
     1801 tctcggcgtt ccattcgtcg acctcgacct cgcgttcgag tccatggcgg gccagacgat
     1861 ccgcgagacg ttcgagaccc gcggcgaacc ttggttccgc gggcgggagg ccgagctgct
     1921 gcggggcacg gcgtcgctcc cctcggcggt catcgcgctc ggcggcggaa cgttcacgtt
     1981 cccggagaac cgcgagttcg tgagggcgca cgggctctcc gtcttcctcg acgtcccctt
     2041 tgccgtgatc gccgagcggc tcgccggcaa gaccgcggac cggccgctgt ttccctccga
     2101 ggagcgagcg cgggaactct gggaggcgcg cctcccgtcc tatagaatgg cggaccggac
     2161 actcgacgtg acacgcgaca tgactgcggc cgacgtggcc ggccgactgg cggatctcgc
     2221 ggcgccgccg ctcacggccc cgggcggagc ggtctgacgt gcgctacctg atcctctcgg
     2281 atctccattc gaacgacgag gcgttgacgg ccgtcctccg ccgcgtgaaa cgcaagaaat
     2341 tcgagcgcgt gatctgcctc ggggacttcg tcggctatgc cgccgacccg aacaaggtcc
     2401 tcgaccgcta ccgcacgttc cggcgcccga cgtacgcgat ccgcgggaac cacgacaagg
     2461 tcgtctcggg cgtcgaggag ggcgcgctct tcaatccgcc cgctctcgag gccgcccgct
     2521 ggacgacgga ccggctttcc cgcgagaacg cggacttcct gaaaaagctc gcgccgggtc
     2581 ccgcgatcgt cgacgggaag ttcgtggtgt gccacggctc gccgctggac gaggacgcgt
     2641 acatcttctc ggacttcgac gcgtcgctga acttcgtcca gatgcaccgg ctcgccgagg
     2701 gggtgaacct gtgtttcttc ggccacagcc acatcccctc gatcttcacg ctcgagcccg
     2761 gcggcatccg cgtcgaggcc gtgcgcgacc cccggtacaa gctgcgcctc gaccccgaga
     2821 agcgttacct cgtgaacccg ggctccgtgg gccagccgcg cgatcgcaac ccgcgcgccg
     2881 cgtacgcgat ctacgattcg gacgcgggcg tcgtccactt cgaccgtgtc ccctacgacg
     2941 tcgggaccac acgccggaag atcgagcggg ccgggctgcc ggccgtgctc ggagaccggc
     3001 tcctcgtcgg cctttgatcc acggcggacg ccgcgccgcg gccacggctg tcgttctcct
     3061 cgcccttgcc agctgcgtgt ctttcggcgg cgcgcccggc ccgccgaagg gcacgtaccg
     3121 agagcgcctc cacggtttcg ccggcctcct cgcgatggaa gaccggcgct cgtacgaccc
     3181 gcttctcgcg ggccgactgg ccgcgtcccc ggacccgtgg ctgcgtgcga agacggcgct
     3241 cgcggccggg cgcctgcgcg acccggacgc gtccatcctc cttccgccgc ttttgacgga
     3301 ccccgaggct tccgttcgcc gcgccgccgc gttcggctcc ggcctctcgg gcgacgcgcg
     3361 gctcgtgccg atcgtggcga ggtcgctccg ggacgccgac gcggcgacgg cggcgaacgc
     3421 cgccgaggcg ctc
//
LOCUS       DAOMFY010000357         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11149_length_3431_cov_7.579976, whole genome shotgun sequence.
ACCESSION   DAOMFY010000357 DAOMFY010000000
VERSION     DAOMFY010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11149_length_3431_cov_7.579976"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..600
                     /gene="rpsU"
                     /locus_tag="PLB01_18505"
     CDS             394..600
                     /gene="rpsU"
                     /locus_tag="PLB01_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQQ79347.1"
                     /translation="MPLIHVKEDESFENALRRFKRKCEKSGILSELKKRQHYEKPSAK
                     RKRKAIAARKKMLRKLAEERRTGM"
     gene            699..3065
                     /locus_tag="PLB01_18510"
     CDS             699..3065
                     /locus_tag="PLB01_18510"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQQ79348.1"
                     /translation="MRLLASEPSADPFEVARRLGVTRDLLARRAWRPAIALAGIDEGA
                     PLVARLGPANRALDPEDLLDLFLLVERAEGARAAVPVEGAELPALTELLRPLADFEDL
                     LSERDPTFEPDGRIKDTASARLYALRAAIQRLRRDLVRKLDELARVQGDALSGGYVTE
                     KGGRYCLPVRSDRREAVQGLVHEKSGSGQTLFVEPLAVVEDNNALAEALEEEREEVHR
                     ILVALTARFSQRRPELTLAVEILTELDAAQARAEFSSASGGVFPEFGDRLVLKGARHP
                     LLDRRLAELRAEVFGEDEGRHADAVPLDLELPEGARTLLLSGPNAGGKSVAMKTAGLF
                     SLLAQSGFAIPAGAGSALPVFDRVLVVAGDAQDLLGDLSSFAAAMTRTARVLAEATPR
                     SLVLLDELGSGTDPDEGAAIAIAVLAEDLARGGFTIATTHLSAVKEWAQDRPGVLSAA
                     MEFDEKAGRPTFHVRPGTFGRSRALAVAERAGLPARVLKAAKARLGNAWEAADAALTR
                     LEAETRRAREAADAAKAAALKAEARLAELDRDRTALTLERAKVKEKAKEQIERALLTL
                     REKTRQELERMREDLKAGRSVSKGALMTVTQSAREAALGLFGEDEPETPAGPVAPGMD
                     VRVAPFGAVGRLLALGARGEAEVEVKGKRMRVDVRSLSPASTAAIGRPNPAGAGRAAS
                     GVPFSKNSSSASSSSDLSGASPAVVATAELVLVGQRVDAALPLVERAINDALLSGKGA
                     LRLVHGHGTGRLAAAVREFLTTHPGVASFRYADASEGGPAVTIARLDV"
     gene            3058..>3431
                     /locus_tag="PLB01_18515"
     CDS             3058..>3431
                     /locus_tag="PLB01_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020285307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HQQ79349.1"
                     /translation="MSDLQYDINDDVKRDVLAATDIVALIGASTSLKKMGKSWKGLCP
                     FHGEKSASFYVHPDKGFYYCFGCGAKGDAITFVRETERLDFPEAVAYLARQAGITLPV
                     RRGGTRADRGRDTRASEALAAAA"
BASE COUNT          486 a         1263 c         1205 g          477 t
ORIGIN      
        1 gtcctcgagc atgagggcga cgacgtcctc gggaaacagg ggcttggcct gcgccgggat
       61 cttgtcggaa agggccgcga gcagggactc gatgtcccgg cgggcgtcca cgagcgacga
      121 cttccacgtc gcgaggtgat cctcgaggtg cgtggaaagc gattcgatca accggcgtgt
      181 cgcggcggga tccaccgaca tacgtctcct gacggcgcca atccggggcc cgtgtggcgt
      241 cgtgtcccgg cattatattc agacaacacg acttgtgcct gcccccggct ccctgtttga
      301 cgggagcgcc ccggcccctt agcatgtggg gtttgcccgc gtccggagac cgccgtccgg
      361 accagtcggg gcgcggaagg agggattcca agagtgccgc tgatccacgt caaagaggac
      421 gagtcgttcg agaacgccct gcgccgtttc aagcgcaagt gcgaaaagtc cggcatcctc
      481 tcggaactca agaagcgtca gcactacgag aagccctcgg ccaagcggaa acgcaaggcc
      541 atcgccgccc gcaagaagat gctccgcaaa ctcgccgaag agcgccgtac cgggatgtag
      601 attcccggtt ggtgtcaccc cttccggctc actccgaact cgactggccc ggcgtgctgc
      661 ggctcgccgg gcgtttttgt tcctcggagc ggggccggat gcgcctgctc gcgtccgagc
      721 cgtcggccga tcccttcgag gtcgcccggc gcctcggggt gacgcgcgac ctcctcgcgc
      781 gccgcgcgtg gaggcccgcg atcgccctcg cgggaatcga cgaaggcgcg ccgctcgtcg
      841 cgcgcctcgg ccccgcgaac cgcgccctcg accccgaaga cctcctcgac ctcttcctcc
      901 tcgtcgaacg cgccgagggc gcccgcgccg cggtgcccgt cgagggtgcg gagctccccg
      961 cgctcacgga gctcctccgc ccgctcgcgg acttcgagga cctcctctcc gagcgcgacc
     1021 cgacgttcga gcccgacggc cggatcaagg acacggcttc ggcgcggctc tacgcgctgc
     1081 gtgcggcgat ccagcgcctg cgccgcgacc tcgtgaggaa gctcgacgag ctcgcgcgcg
     1141 tccagggcga cgccctctcc ggcggctacg tgacggagaa gggcgggcgc tactgcctcc
     1201 ccgtgcgctc ggaccgtcgc gaggccgtgc agggtctcgt gcacgagaag tcgggctcgg
     1261 ggcagacgct gttcgtcgag ccgctcgcgg tcgtcgagga caacaacgcg ctcgccgagg
     1321 ccctcgaaga ggagcgcgag gaagtccacc gcatcctcgt cgcgctcacg gcgcggttct
     1381 cacagcgccg ccccgagctc accctcgccg tggagatcct gacggagctc gacgccgcgc
     1441 aggcgcgcgc ggagttttcg tcggcctcgg gcggcgtgtt ccccgagttc ggggaccgcc
     1501 tcgtcctgaa gggcgcccgc cacccgctcc tcgacaggcg gctcgccgag ctgcgcgccg
     1561 aggtgttcgg cgaggacgag gggcgccacg cggacgcggt gcccctggac ctcgagctgc
     1621 cggagggcgc acggacgctc ctcctgtcgg ggcccaacgc gggcggcaag agcgtcgcga
     1681 tgaagacggc cgggctcttc tcgctcctcg cccagtcggg cttcgcgatt ccggcgggcg
     1741 ccggctcggc gcttccggtc ttcgaccgcg tgctcgtcgt cgccggagac gcccaggacc
     1801 tgctcggcga cctctcgtcc ttcgccgccg ccatgacgcg caccgcgcgc gtcctcgccg
     1861 aggcgacgcc gaggagtctc gtcctcctcg acgagctcgg gagcggcacc gaccccgacg
     1921 agggcgccgc gatcgcgatc gcggttctcg ccgaggacct cgcgcgcggc ggattcacga
     1981 tcgccacgac gcacctctcc gccgtcaagg aatgggcgca ggaccgcccg ggcgtcctct
     2041 cggccgcgat ggagttcgac gaaaaggcgg gccgtccgac gtttcacgtc cggcccggca
     2101 cgttcggccg ctcgcgcgcg ctcgccgtcg ccgagcgcgc cggtctgccg gcgcgcgtcc
     2161 tcaaggcggc gaaggcgcgg ctcggaaacg cgtgggaagc cgccgacgcg gctctcacgc
     2221 ggctcgaggc ggagacccgc cgcgcccggg aggcggccga cgccgcgaag gcggcggcgc
     2281 tcaaggcgga agcgcggctc gccgagctcg accgggatcg caccgcgctc accctcgagc
     2341 gcgcaaaggt caaggagaag gcgaaagagc agatcgagcg ggcgctcctg acgctgcgcg
     2401 agaagacgcg ccaggagctc gagcggatgc gcgaggacct caaagcggga cggagcgtct
     2461 cgaagggcgc gctcatgacg gtgacgcaga gcgcgcgcga ggcggcgctc ggcctcttcg
     2521 gcgaagacga gcccgaaaca ccggccgggc cggtcgctcc gggaatggac gtccgggtcg
     2581 cgccgttcgg agcggtggga aggctgctcg cgctcggggc gcgaggcgag gccgaggtcg
     2641 aggtcaaggg caaacggatg cgcgtcgacg tgaggtcgct ctctcctgcg tccacggccg
     2701 caatcggtcg gccgaacccc gcaggcgcag gccgcgccgc ttccggggtc cccttttcta
     2761 agaactcttc ttcggcttcc tcttcttccg acctctcagg cgcctccccc gccgtcgtcg
     2821 cgacggccga gctcgtcctc gtcggccagc gcgtcgacgc ggctctcccg ctcgtcgagc
     2881 gcgcgatcaa cgacgcgctg ctgtcgggca agggcgcctt gcgcctcgtc cacggccacg
     2941 gcacgggccg tctcgcggcc gccgtgcgcg agttcctgac gacgcacccc ggcgtcgcct
     3001 ccttccgtta cgcggacgcg tccgagggcg gccccgccgt cacgatcgcg aggctcgatg
     3061 tctgacctgc agtacgacat caacgacgac gtcaagcgcg acgtcctcgc cgcgacggac
     3121 atcgtcgccc tgatcggcgc ctcgacgtcg ctcaagaaga tggggaagtc gtggaagggc
     3181 ctgtgcccgt tccacggcga aaagtccgcg tccttctacg tccaccccga caagggcttc
     3241 tactactgct tcggctgcgg cgcgaagggc gacgcgatca cgttcgtgcg cgagaccgag
     3301 cgcctcgact tccccgaggc cgtcgcgtac ctcgcccgcc aggcggggat cacgcttccc
     3361 gtgcgccgcg gcggaacgcg cgcggaccgc gggcgcgaca cgcgcgcctc cgaggcgctc
     3421 gcggcggcgg c
//
LOCUS       DAOMFY010000358         3429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11159_length_3429_cov_8.649378, whole genome shotgun sequence.
ACCESSION   DAOMFY010000358 DAOMFY010000000
VERSION     DAOMFY010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11159_length_3429_cov_8.649378"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /gene="ilvB"
                     /locus_tag="PLB01_18520"
     CDS             <1..1680
                     /gene="ilvB"
                     /locus_tag="PLB01_18520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQQ79350.1"
                     /translation="AARALADELVAQGVTHIFGHPGGAALPLYDALHGSPIRHLLMRH
                     EQGAAHAAQGYARATGRPGVCLATSGPGATNLVTGLTDAMMDSTPVVALTAQVATSLL
                     GRDGFQEADIVGMTQAITKHNWLVLDSEDVAATVRYAFRIAAMGRPGPVLVDVPRDVL
                     QGEASSESTEREGANVVDGPRRADSVSLRRAAFAISNARRPVLYVGGGVISSGASAEV
                     KRLAHKTRIPVTTTLMGKGAFPESDPLSLGMLGMHGTAYANWAMHESDLIIALGARFD
                     DRITGKLATFAPHAKVVHLDVDPVEIGKVRRADFPLYGDAKTVLKQLLPLVGAPHTNG
                     WHDRIAEWKAKAPLAYEQSGDVILPQFAIETLWKMTRDRRPIVTTDVGQHQMWAAQFW
                     KCDEPNTFITSGGLGTMGFGLPAAIGAQCGMPDRLVINLNGDGSFQQTMQAMATAVEE
                     KLPIKVIVLDNQNLGMVRQWQDLFYERRFFAVELKNPDFAKLAEAFGAAAFRVNRPEE
                     LVPAYEAALAVDDRPALVHVRTDGNENCYPMWPAGQSIDSMVVSCPRKKKS"
     gene            1692..1979
                     /locus_tag="PLB01_18525"
     CDS             1692..1979
                     /locus_tag="PLB01_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79351.1"
                     /translation="MQQHAFEIKAKDAPGLMARVETILRRQRMTIRSFSFEGGVGGGP
                     ARIDLVIEADEERSSLVERQLMRLHDVTEVVRMSGAAAFAQPEFTFEEKTA"
     gene            2048..3109
                     /gene="ilvC"
                     /locus_tag="PLB01_18530"
     CDS             2048..3109
                     /gene="ilvC"
                     /locus_tag="PLB01_18530"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQQ79352.1"
                     /translation="MQRTVSRTANSTRNTFMATIWYDKDADIARLKGRTVAIIGYGSQ
                     GHAHALNLRDSGVSVVVGLHATSKSAAKARAEGLTVLPVDEAARRADIVMILTPDTGQ
                     KQLWDEAIAPQMKAGKTAMFGHGFNIRFGQIVPPPDVDVSMIAPKSPGHRVRELYVEG
                     AGTPALLAVEQNASGHAREDALAYAKALGTTRAGVLETTFAEETETDLFGEQAVLCGG
                     TSALVKAAFQTLVDAGYQPEVAYFECLHELKLIVDLMYRGGLNYMRYSISDTAEHGDY
                     TAGPRIVTDATRKEMKKILEEIQEGVYAENWIAENRAGRPNFEATREKERTQLLETVG
                     AELREKMPFLQPVVIGKVA"
     gene            3111..>3429
                     /locus_tag="PLB01_18535"
     CDS             3111..>3429
                     /locus_tag="PLB01_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQQ79353.1"
                     /translation="MHAEGDVRPRIAIFDTTLRDGEQAPGFSMREAEKVRLAVKLDEL
                     GVDILEAGFPIASEDDFSGVRAVAKAVRRPVVAALARAVDADVDRALQALDGCARPRL
                     HTFI"
BASE COUNT          573 a         1203 c         1185 g          468 t
ORIGIN      
        1 gccgcgcgcg cgctggccga cgaattggtc gcgcagggcg tgacgcacat cttcgggcac
       61 ccgggcggcg cggcgctgcc gctctacgac gccctccacg gctcgccgat ccgccacctc
      121 ctcatgcggc acgagcaggg cgccgcgcac gccgcgcagg gctacgcgcg cgcgacgggc
      181 cgccccggcg tgtgcctcgc gacgtccggc ccgggcgcga cgaacctcgt caccgggctg
      241 acggacgcga tgatggactc cacccccgtc gtcgccctga ccgcgcaggt tgcgacgtcg
      301 ctcctcggcc gggacggctt ccaggaagcc gacatcgtcg ggatgacgca ggcgatcacg
      361 aagcacaact ggctcgtcct cgattccgag gacgtcgccg cgacggtccg gtacgcgttc
      421 cgcatcgccg ccatggggcg ccccgggccc gtcctcgtgg acgtgccgcg cgacgtcctg
      481 cagggcgagg cctcttcgga aagcacggag cgcgagggtg cgaacgtcgt cgacggcccg
      541 cgccgggcgg attccgtctc tctgcgccgc gcggcgttcg cgatctcgaa cgcgcggcgc
      601 ccggtcctct acgtcggcgg cggcgtgatc tcgtcgggcg cctcggccga ggtcaagcgc
      661 ctcgcgcaca agacgcgcat cccggtcacc acgacgctca tgggcaaggg cgcgttcccg
      721 gagtcggacc cgctctcgct cgggatgctc gggatgcacg gcaccgcgta cgcgaactgg
      781 gcgatgcacg agagcgacct catcatcgcg ctcggcgcgc gcttcgacga ccgcatcacg
      841 gggaagctcg cgacgttcgc gccgcacgcg aaggtcgtcc acctcgacgt cgaccccgtc
      901 gagatcggga aggtccggag ggccgacttc ccgctgtacg gcgacgcgaa gaccgtgctc
      961 aagcagctgc tcccgctcgt cggcgcaccg cacacgaacg gctggcacga ccggatcgcc
     1021 gagtggaagg cgaaggcgcc gctcgcctac gagcagtcgg gcgacgtcat cctgccgcag
     1081 ttcgcgatcg agaccctctg gaagatgacg cgggaccgcc ggccgatcgt cacgacggac
     1141 gtcggccagc accagatgtg ggccgcgcag ttctggaagt gcgacgagcc gaacacgttc
     1201 atcacgtcgg gcggcctcgg gacgatgggc ttcgggctgc cggccgcgat cggcgcgcag
     1261 tgcgggatgc cggaccgcct cgtgatcaac ctgaacggcg acggcagctt ccagcagacg
     1321 atgcaggcga tggcgacggc ggtcgaggag aagctcccga tcaaggtcat cgtcctcgac
     1381 aaccagaatc tcgggatggt ccgccagtgg caggacctct tctacgagcg ccgcttcttc
     1441 gcggtcgagc tcaagaaccc cgacttcgcg aagctcgccg aggcgttcgg cgccgcggcg
     1501 ttccgcgtga accgccccga ggagctcgtc ccggcctacg aggccgcgct cgccgtggac
     1561 gaccggcctg cgctcgtcca cgtgcggacg gacggcaacg agaactgcta ccccatgtgg
     1621 cccgccggcc agtccatcga ctcgatggtg gtttcctgcc cccggaagaa gaagagctga
     1681 ggaggatcgt catgcagcag cacgcattcg agatcaaggc gaaagatgct cccgggctca
     1741 tggcgcgagt cgagacgatc ctgcggcgcc agcgcatgac gattcggagc ttttccttcg
     1801 aaggtggtgt gggtggcggc cccgcccgga tcgatctcgt catcgaggcg gacgaggagc
     1861 gctcgagcct cgtcgaacgt cagctcatgc ggctccacga cgtgacggaa gtcgtccgga
     1921 tgagcggagc ggccgccttc gcgcagcctg aattcacttt cgaagagaag actgcttaga
     1981 aggtcccgag caggccgcac ggagcgatcg aaccctgcgg cacgccctct ttaccttcta
     2041 agaagaaata caacgaaccg taagccgaac agccaactcg acgaggaaca ctttcatggc
     2101 cacgatctgg tacgacaagg acgccgacat cgcccgcctc aagggccgca cggtcgcgat
     2161 catcggttac ggcagccagg ggcacgcgca cgcgctgaac cttcgcgaca gcggggtctc
     2221 ggtcgtcgtg ggcctccacg cgacgtcgaa gtccgccgcg aaggcgcgcg ccgagggcct
     2281 cacggtcctg ccggtggacg aggcggcgag gcgcgccgac atcgtcatga tcctcacgcc
     2341 ggacacgggc cagaagcagc tctgggacga ggcgatcgcg ccgcagatga aggccggcaa
     2401 gaccgcgatg ttcgggcacg gcttcaacat ccggttcgga cagatcgtcc cgccgcccga
     2461 cgtggacgtc tcgatgatcg cgccgaagtc gcccggccac cgggtccgcg agctgtacgt
     2521 ggagggcgcg ggcacgccgg cgctcctcgc cgtcgagcag aacgcgagcg gccacgcgcg
     2581 cgaggacgcg ctcgcgtacg cgaaagctct cgggacgacg cgcgcgggcg tcctcgagac
     2641 gacgttcgcc gaggagacgg agacggacct gttcggcgag caggcggtcc tctgcggcgg
     2701 cacgtcggcc ctcgtgaagg ccgcgttcca gacgctcgtc gacgcgggat accagcccga
     2761 ggtcgcctac ttcgagtgcc tccacgagct gaagctcatc gtcgacctca tgtaccgggg
     2821 cggcctgaac tacatgcgct actcgatcag cgacacggcc gagcacggcg actacaccgc
     2881 cggcccgcgc atcgtgaccg acgcgacgcg caaggagatg aagaagatcc tcgaggaaat
     2941 ccaggagggc gtctacgccg agaactggat cgccgagaac cgcgccggcc gcccgaactt
     3001 cgaggcgacg cgcgagaagg agcgcaccca gctcctcgag acggtcggcg ccgagctgcg
     3061 cgagaagatg ccgttcctcc agccggtcgt catcgggaag gtggcctgac atgcacgccg
     3121 agggcgacgt tcggccgcgc atcgcgatct tcgacacgac gctccgggac ggcgagcagg
     3181 cgccgggctt ctcgatgcgc gaggcggaaa aggtgcgcct cgccgtcaag ctcgacgagc
     3241 tgggcgtgga catcctcgag gccgggttcc cgatcgcctc ggaggacgac ttctcgggcg
     3301 tccgcgccgt cgcgaaggcc gtccgccgtc ccgtggtggc cgcgctcgct cgcgccgtgg
     3361 acgccgacgt ggaccgtgct ctccaggcgc tcgacggctg cgcgcggccg cgcctccaca
     3421 cgttcatcg
//
LOCUS       DAOMFY010000359         3405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11334_length_3405_cov_9.779403, whole genome shotgun sequence.
ACCESSION   DAOMFY010000359 DAOMFY010000000
VERSION     DAOMFY010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11334_length_3405_cov_9.779403"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLB01_18540"
     CDS             <1..518
                     /locus_tag="PLB01_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79354.1"
                     /translation="RSLARSIGVAFPLEAGDFGAPFPPGVQGLLPAASVLDAVTSRFL
                     LLAGGAAFGALLLADVLRKPAVRALMAVFAAGAFAPLDARTWGELLVPVLAGSIVGAA
                     FLAALATLLRDDPRAYVFTAAGLGLAGGAADLLTSGVPWWTVNGALAAAVLVGLLAWR
                     GFDRPAPAAFP"
     gene            605..3292
                     /locus_tag="PLB01_18545"
     CDS             605..3292
                     /locus_tag="PLB01_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQQ79355.1"
                     /translation="MNPSIVRARACGLLLALALAVPGALAQAAAPPLLDRELFFGNPE
                     IAGAQLSPDGKYIAFLKPWKDTRNVWVKKTGEGYDKAHLVTADPKRPIPGFFWTRDSK
                     KILFVQDKDGDENYNVYAVDPAAAPEAGKDAPAARNLTDAKGARAAIYAVPKADPDTI
                     YVGLNDRDAAWHDVYKVKISTGERTLVRKNTEKIAGWVFDNAGKLQLAARTSDKGDTE
                     ILRVDADAFKLVYTCTVFESCGPVRFHKDNARVYMETNKGAPDLVQLVLFDPATGKEE
                     VVESDPMKRVDFGSAIFSDATDQLVATTYEDDRTRLYFRDKNWEADYNLLKKKLGDKE
                     VLPNSSTADESVWLVTASSDTDPGSRYLFDRKTKKLSLEYVARERIPREHMAPMKAIR
                     YKSSDGLEIPAYLTLPKGVAPKNLPLVVFPHGGPWARTSWGFSNYAEFLANRGYAVLQ
                     PNFRSSTGYGKKFLNAGNKQWGDLMQDDLTWGVKHLVAEGVVDPKRVAIMGGSYGGYA
                     TLAGVAFTPDLYAAGVAIVAPSNLLTLLETIPPYWESGRVIFHARMGDPTTPEGKKQL
                     ERQSPLNSAAKIKTPLLVVQGANDPRVKKAESEQIVIALRDRGFPVEYILAPDEGHGF
                     ARPVNNMAMMAAAEAFLAKRIGGRFQEGGTPEVVARLKEITVDPKTVVLSKAVDAAAV
                     GAPKPVSGLVPGTYKYAGAIQAGAQTIPISGVNTVTEEPGTYTVNYTMKLPMGEANDT
                     VTLDKATLQPVKRSVKQGQVSMDLAYKDGKVTGSVAMGGEPKPVDASTGGALYADGSA
                     AWESIAHLPLAEGYAVTFRNFDVQKQKPSLKQVKVLGAEELTVPAGTFKTWKAEVTSA
                     EGEPGSTTVWVAQDSRKVVKTSSVVPQMGGATVTSELQP"
BASE COUNT          561 a         1223 c         1148 g          473 t
ORIGIN      
        1 cccgctccct cgcccggtcg atcggggtcg ccttcccgct cgaggccggc gattttggcg
       61 ccccgttccc gccgggcgtg cagggcctgc ttccggccgc gtccgtgctc gacgccgtga
      121 cgtcgcggtt cctgctcctc gcgggcggcg ccgcgttcgg cgcgctcctc ctcgcggacg
      181 tcctgaggaa gccggcggtc cgcgccctca tggccgtctt cgcggcgggc gccttcgctc
      241 cgctcgacgc gcggacgtgg ggagagctac tcgtgccggt cctcgcgggc tcgatcgtgg
      301 gcgccgcgtt cctcgcggcg ctcgcgaccc tgcttcggga cgacccccgg gcgtacgtct
      361 tcacggccgc cggcctcgga ctcgcgggcg gggccgccga cctcctgacg agcggcgtcc
      421 cctggtggac ggtgaacggc gccctggccg cagccgtcct cgtcgggttg ctcgcgtggc
      481 gcggcttcga ccgcccggcg cctgccgcgt ttccctgaac gatgccttcg agaacccgtc
      541 tcagaaccgg gtgccttcgc cctgcgtata accctctgga ccccacacat ttgaggagga
      601 atccatgaat ccgtcgatcg ttcgagcccg cgcgtgtggg cttttgctcg cattggccct
      661 tgccgtgccg ggcgcactcg cccaggcggc ggccccgccg cttctcgacc gcgagctctt
      721 cttcggcaac ccggagatcg ccggggcgca gctgtccccg gacggcaagt acatcgcgtt
      781 cctgaagccc tggaaggaca cacggaacgt ctgggtcaag aagacgggcg agggctacga
      841 caaggcccac ctcgtcacgg cggacccgaa gcggccgatt cccggattct tctggacgcg
      901 cgacagcaag aagatcctct tcgtccagga caaggacgga gacgagaact acaacgtcta
      961 tgccgtcgac ccggccgcgg cgccggaggc cggcaaggac gcgccggccg cccgcaacct
     1021 gacggacgcc aagggagccc gcgcggccat ctacgccgtg ccgaaggccg accccgacac
     1081 gatctacgtc ggcctcaacg accgcgacgc cgcctggcac gacgtctaca aggtgaagat
     1141 ctcgaccggc gagcggacgc tcgtgaggaa gaacacggag aagatcgccg gctgggtctt
     1201 cgacaacgcc gggaagctcc agctcgccgc gcgcacgtcc gacaagggcg acacggagat
     1261 cctccgcgtg gacgccgacg cgttcaaact cgtctatacg tgcacggtct tcgagagctg
     1321 cggccccgtc cggttccaca aggacaacgc gcgcgtctac atggagacga acaagggcgc
     1381 gcccgacctc gtccagctcg tcctcttcga cccggcgacg ggcaaggagg aggtcgtcga
     1441 gagcgacccg atgaagcggg tcgacttcgg cagtgcgatc ttctcggacg ccacggacca
     1501 gctcgtcgcg acgacgtacg aggacgaccg cacgcgcctc tacttccggg acaagaactg
     1561 ggaggccgac tacaacctcc tcaagaagaa gctcggcgac aaggaagtcc tcccgaactc
     1621 ttcgacggcc gacgagagcg tctggctcgt gaccgcgtcg agcgacacgg accccggttc
     1681 gcgttacctc ttcgaccgga agacgaagaa gctcagcctc gagtacgtcg cccgtgagcg
     1741 gatcccgcgc gagcacatgg ccccgatgaa ggccatccgg tacaagtcct cggacggcct
     1801 cgagatcccg gcgtacctca cgctgcccaa aggagtcgcg ccgaagaatc ttccgctcgt
     1861 cgtcttcccg cacggcggcc cttgggcgcg cacgagctgg ggcttcagca actacgccga
     1921 gttcctcgcg aaccgcggct acgccgtgct gcagccgaac ttccgctcct cgaccggata
     1981 cggcaagaag ttcctgaacg ccggcaacaa gcagtggggc gacctcatgc aggacgacct
     2041 cacgtggggc gtcaagcacc tcgtcgccga gggcgtcgtc gacccgaagc gcgtcgcgat
     2101 catgggcggc tcgtacggcg gctacgcgac cctcgcgggc gtcgccttca cgccggacct
     2161 gtacgcggcc ggcgtcgcga tcgtcgcgcc gtcgaacctc ctcacgctcc tcgaaacgat
     2221 ccctccgtac tgggagtccg gccgcgtgat cttccacgcg cgcatgggcg acccgacgac
     2281 gcccgagggc aagaagcagc tcgagcgcca gtcgccgctc aactccgcgg cgaagatcaa
     2341 gacgccgctc ctcgtcgtgc agggcgcgaa cgacccgcgc gtcaagaagg ccgagtccga
     2401 acagatcgtg atcgcgctgc gcgaccgcgg tttcccggtc gagtacatcc tcgccccgga
     2461 cgagggccac gggttcgcac ggcccgtgaa caacatggcc atgatggcgg cggccgaggc
     2521 gttcctcgcc aagcgcatcg gcgggcgctt ccaggagggc ggcacgcccg aggtcgtcgc
     2581 gcggctcaag gagatcacgg tcgacccgaa gaccgtcgtc ctctcgaagg cggtcgacgc
     2641 cgcggccgtc ggcgcgccga agcccgtctc gggcctggtg ccggggacgt acaagtacgc
     2701 cggcgcgatc caggccggag cgcagacgat cccgatcagc ggcgtgaaca cggtgaccga
     2761 ggagccgggg acgtacaccg tcaactacac gatgaagctc ccgatgggcg aagcgaacga
     2821 caccgtgacg ctcgacaagg ccacgctgca gcccgtcaag cgctccgtca aacagggcca
     2881 ggtgtcgatg gacctcgcgt acaaggacgg gaaggtgacc gggtccgtcg cgatgggcgg
     2941 cgagccgaag ccggtggacg cgtcgacggg cggcgccctc tacgcggacg gctcggcggc
     3001 gtgggaatcc atcgcgcacc tgccgctcgc cgaaggctac gcggtcacgt tccgcaactt
     3061 cgacgtccag aagcagaagc cctcgctcaa gcaggtcaag gtcctcggcg ccgaggagct
     3121 caccgtcccc gccggcacgt tcaagacgtg gaaggcggag gtgacgtcgg ccgagggcga
     3181 gccgggctcg acgaccgtgt gggtcgcgca ggactcgcgc aaggtcgtca agacgagctc
     3241 cgtcgtcccg cagatgggcg gcgcgacggt gacgtcggag ctgcagccct agtcccggga
     3301 tcaggggcgc cgggccgctg tcatccgcgg ccgccggccc ggcgccactc ccagtccgcg
     3361 aggcgctcgc gggcgccgag cccgatcgcg cggtgcagct cgagg
//
LOCUS       DAOMFY010000360         3394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11400_length_3394_cov_6.942498, whole genome shotgun sequence.
ACCESSION   DAOMFY010000360 DAOMFY010000000
VERSION     DAOMFY010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11400_length_3394_cov_6.942498"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..785
                     /locus_tag="PLB01_18550"
     CDS             102..785
                     /locus_tag="PLB01_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ79356.1"
                     /translation="MGETEVRALDGVTIKVEPGEFLAVMGPSGSGKSTFMNIVGCLDR
                     PTGGRYVLEGVDVSSLGRDARAEIRNDKIGFVFQNFNLLSRTSALENVELPLLYSRKA
                     KLTDEEARARAMRCLQRVGLGDRWDHTSAQLSGGQQQRVAIARSLVNEPAILLADEPT
                     GNLDSRTSEEVMAIFQALNDEGKTVVLITHEPDIAQFARRIVTFRDGKVLSDVAVKDR
                     KRAAGGKPA"
     gene            782..2008
                     /locus_tag="PLB01_18555"
     CDS             782..2008
                     /locus_tag="PLB01_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ79357.1"
                     /translation="MKKTGNILKVAFRALARNKLRSLLTALGIIIGVACVIATIGIGE
                     GARLQTENQLKSLGTNFLMIFPGTTTSSGARSGSGSNSKLSQDDVDAIRREVGSVAYV
                     SATIRTVAQVIYGNQNWSTSIQGGEVDWPLIRSWNLASGQFFTDQDNRSASKVAVLGK
                     TVATNLFGDEDPIGKMIRIKNIPFKVTGVLEAKGGSMMGQDQDDIVIAPYETVRKKLM
                     GTTAVGAILASAASNEQVAIAQDEITALLRQRHRINKAAGQDDDFMMRSQTEMLQQAE
                     AQSRTMSTLLWSIAGVSLLVGGIGIMNIMLVSVTERTREIGVRMAIGAKGGDIRAQFL
                     VEAVALSLAGGTVGIGLGLGIQRFVAKVAGWPVALEPTAIGLAFAFSAVVGITFGFYP
                     ALKASRLDPIEALRYE"
     gene            2024..>3394
                     /locus_tag="PLB01_18560"
     CDS             2024..>3394
                     /locus_tag="PLB01_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79358.1"
                     /translation="MNTEKPGIEDSGAAPARGAAPHEARGVWSPRRSLGARLVLYLLP
                     VAVVPALVYWLLVDRETDRSERQMLETLLADAHRHEALTLRDEADGAVRSIGDFVRKL
                     ETIVRRAGVETARALEAGPEADRAAEELVDEPGGPLRSARPGASIAMVSRKVGLTAQA
                     RRDLAATRRLEAPFVSLGLGPVDVSAIAVRTASGVMRAVPGRPLQVGGTNVVEPDFTF
                     PSEVTRALAAVPAPEGPAPVAWSAVYDDLFAQSGKIVTAATLVRGGDGRPVAEVGVDW
                     VFARLFESPPDPSRPDDAEILFAEDGTLLLAAPPGRVAEADLRVLSEAVRKETASDLE
                     AELAGRRFLVEVRRLPVPRWIYARVSPRAALEKKVRAQLDPIFAAGRKRRAELRLLYL
                     AVIVALSAGLVAVTSRALAPVRRVARATRRLRLGARTPRQRDQRDLAFAGGNRLGGTT
                     DMNQIGA"
BASE COUNT          507 a         1193 c         1203 g          491 t
ORIGIN      
        1 ggcgggggcc cgggcggcgg cgggcggcgg cccttctgag cgggcggccg gccgtggcgc
       61 agcccgtcat cgagctcgag aacctcacgc gcgtctacca gatgggcgag accgaggtgc
      121 gcgccctcga cggcgtgacg atcaaggtcg agcccggcga attcctcgcg gtcatgggcc
      181 catcgggctc ggggaagtcg acgttcatga acatcgtcgg ctgcctcgac cggccgacgg
      241 ggggtcggta cgtcctcgag ggcgtcgacg tgtcgtcgct cgggcgcgac gcgcgcgccg
      301 agatccggaa cgacaagatc ggtttcgtct tccagaactt caacctcctc tcgcgcacgt
      361 ccgcgctcga gaacgtggag cttccgctcc tctactcgag gaaggcgaag ctcacggacg
      421 aggaggcccg ggcccgcgcg atgcggtgcc tccagcgggt ggggctcggc gaccgctggg
      481 accacacgtc cgcccagctc tcgggcggcc agcagcagcg cgtcgcgatc gcgcggagcc
      541 tcgtgaacga gcctgcgatc ctcctcgcgg acgagccgac gggcaacctc gactccagga
      601 cctccgagga ggtcatggcg atcttccagg ccctcaacga cgaggggaag acggttgtcc
      661 tcatcacgca cgagcccgac atcgcgcagt tcgcccgccg catcgtcacc ttccgcgacg
      721 gcaaggtgct gagcgacgtc gccgtaaagg accgcaagcg cgcggccgga ggcaagcccg
      781 catgaagaag accggcaaca tcctgaaggt cgcgttccgc gcgctcgcgc gcaacaagct
      841 ccggtcgctc ctcaccgcgc tcggcatcat catcggcgtc gcgtgcgtca tcgcgacgat
      901 cgggatcggc gagggcgcgc gcctgcagac cgagaaccag ctgaagtccc tcgggacgaa
      961 ctttctcatg atcttcccgg ggacgaccac gtcctccggg gcccgttcgg gctcggggag
     1021 caactcgaag ctctcacagg acgacgtgga cgcgatccgc cgcgaggtcg ggtccgtggc
     1081 ctacgtctcc gcgacgatcc gcacggtcgc gcaggtcatc tacgggaacc agaactggtc
     1141 cacctccatc cagggaggcg aggtcgactg gccgctcatc cgctcgtgga acctcgcgtc
     1201 gggccagttc ttcaccgacc aggacaaccg ctccgcctcg aaggtggccg tgctcggaaa
     1261 gaccgtcgcg acgaacctgt tcggcgacga ggacccgatc ggcaagatga tccggatcaa
     1321 gaacatcccg ttcaaggtga ccggcgtcct cgaggccaag ggcggctcga tgatgggaca
     1381 ggaccaggac gacatcgtga tcgcgcccta cgagacggtc cgcaagaagc tcatgggcac
     1441 gacggcggtc ggcgcgatcc tcgcgtcggc ggcgtcgaac gagcaggtcg ccatcgccca
     1501 ggacgagatc acggcccttc tccggcagcg ccaccgcatc aacaaggcgg cggggcagga
     1561 cgacgacttc atgatgcgct ctcagaccga gatgctccag caggccgagg cgcagagccg
     1621 cacgatgtcg acgctcctct ggtcgatcgc gggcgtgtcg ctcctcgtcg gcgggatcgg
     1681 gatcatgaac atcatgctcg tctcggtcac ggagaggacg cgcgagatcg gcgtgaggat
     1741 ggcgatcggc gccaagggcg gcgacatccg cgcgcagttc ctcgtcgagg ccgtcgcgct
     1801 ctcgctcgcg ggcggcacgg tggggatcgg cctcggactc gggatccagc gcttcgtcgc
     1861 gaaggtcgcg ggctggcccg tcgcgctcga gccgaccgcg atcgggctcg cgttcgcgtt
     1921 ctcggccgtc gttgggatca cgttcggttt ctacccggcc ctgaaggcgt cgaggctgga
     1981 ccccatcgag gccctgcgct acgagtagcg ttaaccttcc ggcgtgaaca cggagaagcc
     2041 ggggatcgag gactcgggcg cggctcccgc ccgcggggcg gcgccgcacg aagcacgcgg
     2101 ggtgtggagc ccccgccgtt ccctcggcgc gcggctcgtg ctgtacctcc ttccggtggc
     2161 cgtggtgccc gcgctcgtct actggctcct cgtcgaccgg gagacggatc ggagcgagcg
     2221 gcagatgctc gaaacgctcc tcgcggacgc gcatcggcac gaggcgctga ccctccgcga
     2281 cgaggccgac ggcgccgttc ggtcgatcgg agacttcgta cggaagctcg agacgatcgt
     2341 gcgccgagcg ggcgtcgaga cggcgcgtgc tctcgaggca ggccccgaag cggaccgtgc
     2401 cgcggaggaa ctcgtggacg agccgggcgg tcccctccgg tcggcccggc ccggcgccag
     2461 catcgcgatg gtgagccgca aggtcgggct gaccgcccag gcgcgccgcg acctggcggc
     2521 gacccgccgt ctcgaggcgc cgttcgtcag cctcggcctc gggcccgtcg acgtgtcggc
     2581 aatcgccgtg cggacggcgt caggcgtgat gcgcgcggtg cccggacgtc cgctgcaggt
     2641 cgggggcacg aacgtcgtcg agccggattt cacgttcccc tccgaagtga cccgcgcgct
     2701 cgcagccgta cccgcgccgg agggccccgc gcccgtcgcg tggtccgccg tctacgacga
     2761 cctgttcgca cagtccggaa aaatcgtcac cgcggcgacg ctcgtgcgcg gaggcgacgg
     2821 ccggcctgtc gccgaggtcg gcgtggactg ggtgttcgcg cgattgttcg agtcgccgcc
     2881 ggacccctcc cggccggacg acgcggagat cctcttcgcg gaagacggga cgcttctgct
     2941 cgcggcgccc cccgggcgcg tggcggaagc cgatcttcgc gtcctctccg aggccgtccg
     3001 caaggaaacc gcgtcggacc tcgaggcgga gctggcgggc cggcgtttcc tcgtcgaggt
     3061 tcgccgcctc ccggtgccgc gatggatcta tgcgagggtc tcgccgcgcg ccgcgctcga
     3121 aaagaaggtc cgcgcccagc tggatccaat ctttgcggcc ggccgcaagc ggcgcgccga
     3181 gctgcggctt ctctacctcg ccgtcatcgt cgcgctgtcg gcgggcctcg tcgcggtgac
     3241 gagccgcgcc ctcgccccgg tccggcgcgt tgcgcgcgcc acgcggaggc tgcgcctcgg
     3301 tgcgcggacg ccccgccagc gtgatcagcg cgatcttgca tttgccggcg gcaatcgcct
     3361 cggcggcacg accgacatga atcagatagg agca
//
LOCUS       DAOMFY010000361         3386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11451_length_3386_cov_6.160312, whole genome shotgun sequence.
ACCESSION   DAOMFY010000361 DAOMFY010000000
VERSION     DAOMFY010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11451_length_3386_cov_6.160312"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..87)
                     /locus_tag="PLB01_18565"
     tRNA            complement(11..87)
                     /locus_tag="PLB01_18565"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Ala,seq:cgc)
     gene            complement(155..661)
                     /locus_tag="PLB01_18570"
     CDS             complement(155..661)
                     /locus_tag="PLB01_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79359.1"
                     /translation="MKAALKFLAFCLVAVVVMAMVGAGLPQQHVLTRRKSFAAPPERV
                     WSALLSIRQLPVDRSDMRAVDQGVATAPPSAIEVVGTPVAITIETFRPPRTLVVRTSE
                     PDVSYGGTWTFELELETSQITKLTVTEEAVVRGRLLRFVVRTFGLDDALVEGVFRAVK
                     RKLVETPS"
     gene            complement(658..1929)
                     /locus_tag="PLB01_18575"
     CDS             complement(658..1929)
                     /locus_tag="PLB01_18575"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HQQ79360.1"
                     /translation="MHPTTAQLRLARTWRQNLWETLGVVGRGTVRRFLDARGLDLASS
                     LAYSSLLTLVPLIASVTVLTSTFFGLSGVGIYRILRLALPGANREVVADLQTLVRYAS
                     VSGSATIFFLVTSLRTYFLVEGAAKALWGTTVTPKPPLRRIGTALSVMILGPIAIGVL
                     TSLLLESGGGFSGFRPLGSFLTLVLLVYLYRTLPGAAVRWGPALAGATFAAATITSLR
                     MAITRGVAQLAGAGAVYGPLTAAIVFVVAVGFVFVLFLLGISLAHAIQFRAEFLDHDA
                     LAERTEEGGRLYESVRLLLVLTVAWRNDRATRTLTAIAHELERPEADVVPLVRDLRQA
                     GLVTAGEGGVFSLTRAPETISLYAVARAIGESAARAVPRGTDAVATSLHRIFGKAARE
                     ERAVLQGTSLHDLLTAGAGEQNPTVKALVTP"
     gene            2001..2291
                     /locus_tag="PLB01_18580"
     CDS             2001..2291
                     /locus_tag="PLB01_18580"
                     /inference="COORDINATES: protein motif:HMM:NF039524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HQQ79361.1"
                     /translation="MANNTVEDAARAVEQKIDATREAAASGAGRVRERVSEVANDVKA
                     RASALRDKIADTEWEDVKANVAGYVRDNPGKSVAIALGVGFALGLLMRRRDD"
     gene            2291..2575
                     /locus_tag="PLB01_18585"
     CDS             2291..2575
                     /locus_tag="PLB01_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79362.1"
                     /translation="MDGAVRPPAPHGASPEGPVGAPGEPFGFIASADWQKTVLETVQK
                     HPIPCLVGAAAVGFLVGRYRGKAIVGALAGVAANMVLRQIGESFVGGDIG"
     gene            2756..>3386
                     /gene="selD"
                     /locus_tag="PLB01_18590"
     CDS             2756..>3386
                     /gene="selD"
                     /locus_tag="PLB01_18590"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQQ79363.1"
                     /translation="MKLLGALPRVRDPRALVGFEASDDAAVFRLGEAGDADAECLLAT
                     TDFFTPVVDSPFDFGRVAAANALSDVWAMGGEPLFALNLVGFPVKALPMEVLGEILAG
                     GAAVAGDAGIPILGGHSTDFDVPVYGMAVTGRVKASRLRRNVGARPGDALVLTKALGT
                     GILTSALRARVLREGSIRALLGRGEGPTPEEEAGAVASMDAVTLRVSGVP"
BASE COUNT          518 a         1140 c         1279 g          449 t
ORIGIN      
        1 cgacgtaaag tggtggagct gaacgggatc gaaccgtcga cctcctgagt gcgattcagg
       61 cgctctccca aactgagcta cagccccacc cgagccggag aggacgcgga gattagcggg
      121 aagccgcggg gccgtcaaac gggccccgcg agcttcagct tggagtctcg acgagcttgc
      181 gcttgacggc gcggaacacg ccctcgacga gcgcgtcgtc gaggccgaac gtccgcacga
      241 cgaaacgcag gaggcgtccg cgcaccaccg cctcctcggt cacggtgagc ttcgtgatct
      301 gcgaggtctc gagctcgagc tcgaacgtcc acgtcccgcc gtaggagacg tccggctcgg
      361 acgtccggac gacgagcgtg cgcggcgggc ggaacgtctc gatcgtgatc gcgaccggcg
      421 tcccgacgac ttcgatcgcg gagggcggcg ccgtggcgac gccctggtcc acggcgcgca
      481 tgtccgagcg gtcgacgggc agctggcgga tcgagaggag cgcggaccag acccgttcgg
      541 gcggcgccgc gaacgacttg cgtcgcgtca ggacgtgctg ctgcggcagt cccgctccga
      601 ccatcgccat gacgacgacg gcgacgaggc agaacgcgag gaacttgagg gcggctttca
      661 tggcgtcacg agcgccttca cggtcggatt ctgctcccct gcgcccgccg tgaggaggtc
      721 gtgcaggctc gtcccctgca ggacggcccg ctcctcgcgc gccgccttcc cgaagatgcg
      781 gtgcaggctc gtggcgacgg cgtcggttcc cctcgggacg gcgcgcgcgg ccgactcgcc
      841 gatcgcgcgc gcgacggcgt agagcgagat cgtttccggg gcccgcgtga gcgagaagac
      901 gccgccctcg ccggcggtca cgaggccggc ctggcgaagg tcgcgcacga gcggcacgac
      961 gtcggcctcc ggccgctcga gctcgtgcgc gatcgccgtg agcgtgcgcg tcgcgcggtc
     1021 gttccgccag gcgaccgtga ggacgaggag gagccggacg gactcgtaga gccggccgcc
     1081 ttcctcggtg cgctcggcga gggcgtcatg gtcgaggaac tcggcgcgga actggatcgc
     1141 gtgcgcaagc gagattccga ggaggaagag gacgaagacg aatcccaccg cgacgacgaa
     1201 gacgatggcg gccgtgagcg ggccgtagac ggcgcccgcg cccgccagct gcgcgacgcc
     1261 gcgggtgatc gccatccgga gcgacgtgat ggtcgcggcc gcgaacgtcg caccggcgag
     1321 cgcggggccc cagcggacgg cggcgcccgg gagcgtccgg tagaggtaga cgagaaggac
     1381 gagcgtcagg aaggagccca ggggccggaa gcccgagaac ccacccccgc tctcgaggag
     1441 gagcgacgtg agaacgccga tcgcgatcgg tccgaggatc atgacggaca gcgcggttcc
     1501 gatccggcgg agcgggggct tcggcgtgac ggtcgttccc cagagggcct tcgccgcgcc
     1561 ctccacgagg aagtacgtcc tcagggatgt cacgaggaag aagatcgtgg ccgagccgga
     1621 caccgacgcg tagcgcacga gcgtctgcag gtccgcgacg acctcgcggt tcgccccggg
     1681 cagggcgagg cggaggatcc ggtagatccc gacgcccgag agcccgaaga aggtggacgt
     1741 caggacggtg accgacgcga tgagcgggac cagggtgagt agcgacgagt acgcgaggct
     1801 cgacgcgagg tcgagcccgc gcgcgtccag aaagcggcgc accgttcccc ggcccacgac
     1861 gccgagcgtc tcccagaggt tctgccgcca ggtgcgggcg agccggagct gcgccgtcgt
     1921 cgggtgcatg ccgcctccca tcgtaacctt ccgcccgcgc acgggctgtg acaagattcg
     1981 gcctcccgaa ggagggcctc atggccaaca acaccgtcga agacgccgcc cgggcggtgg
     2041 agcagaagat cgacgcgacg cgcgaggccg cggcatcggg cgcgggccgg gtccgcgagc
     2101 gcgtctcgga agtcgcgaac gacgtcaagg cgcgcgcctc ggcgctgcgc gacaagatcg
     2161 cggacaccga gtgggaggac gtgaaggcga acgtcgcggg ctacgtccgt gacaacccgg
     2221 gcaagtcggt cgcgatcgcg ctcggcgtcg gtttcgcgct cggacttctc atgcggcgga
     2281 gggacgactg atggacggcg cggtccggcc gcccgctccg cacggcgcct cgccggaagg
     2341 cccggtcggc gcgcccggcg aaccgttcgg cttcatcgcc tcggccgact ggcagaagac
     2401 cgtcctcgag accgtccaga agcatccgat cccgtgcctc gtcggcgcgg cggcggtcgg
     2461 cttcctcgtc ggccgctacc gcggcaaggc catcgtcggc gcgctcgcgg gcgtcgcggc
     2521 gaacatggtg ctccgccaga tcggcgagtc gttcgtgggc ggagacatcg gctaggagat
     2581 tcggattcgg acgaggattc ggattcggat ttcctagaag ggtgagtgag gcgggagcac
     2641 gctcacgcgg cccgcgtaca ctcttccgat ggaaaaggcc gcgaaggctc atccgtcgat
     2701 cctgacgttc gcgtcgtcgg cgggttgagc gtgcaagctg ggtcagtctg acctggtgaa
     2761 actgctgggt gcgctgccgc gggtccgcga cccgcgcgct ctcgtcggat tcgaagcctc
     2821 cgacgacgct gccgtattcc gcctcgggga ggccggcgac gccgacgccg agtgcctgct
     2881 cgcgacgacg gacttcttca cgcccgtcgt cgactcgccg ttcgacttcg gccgcgtcgc
     2941 ggccgcgaac gcgctctcgg acgtgtgggc gatgggcggc gagccgctct tcgcgctgaa
     3001 cctcgtcggc ttcccggtga aggcgctccc gatggaggtc ctcggagaga tcctcgcggg
     3061 cggcgccgcg gtggccgggg acgcggggat cccgatcctg gggggacaca gcacggattt
     3121 cgacgtcccg gtctacggca tggccgtgac ggggcgcgtg aaggcgtcgc gcctgcgccg
     3181 caacgtcggc gcccggccgg gcgacgcgct cgtcctgacg aaggcgctcg gcaccggcat
     3241 cctcacgtcc gcgctgcgcg cgcgcgtgct gcgcgagggc tcgatccgcg cgcttctcgg
     3301 ccgcggcgag ggcccgactc ctgaggagga ggcgggcgcg gtcgcctcga tggacgcggt
     3361 gacgttgagg gtgtccggcg tgccct
//
LOCUS       DAOMFY010000362         3369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11556_length_3369_cov_7.362100, whole genome shotgun sequence.
ACCESSION   DAOMFY010000362 DAOMFY010000000
VERSION     DAOMFY010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11556_length_3369_cov_7.362100"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLB01_18595"
     CDS             complement(<1..721)
                     /locus_tag="PLB01_18595"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HQQ79364.1"
                     /translation="MKKEFSFVAAGARTDVVVGEGALASLGARLPSIAPGRWFLVSSK
                     RIFLLHGHLLSAGGAERVLSKEPLFVPDGEKAKTWSVLGRLIEQLVRRGLRRDGGVVC
                     LGGGTVGDVGGMAAALALRGVPVVQAPTTLLAACDSALGGKTAVDLPQGKNLAGAFHA
                     PRLVLADTAVLDTLPERAFRSGLAEVVKTSFLDARFHRAMARLAGPLASREGAAVAEA
                     VFRSLRVKAAVVSADPFETSGV"
     gene            complement(761..2524)
                     /gene="aspS"
                     /locus_tag="PLB01_18600"
     CDS             complement(761..2524)
                     /gene="aspS"
                     /locus_tag="PLB01_18600"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQQ79365.1"
                     /translation="MSALSRTPAGSLRPSHAGQTVRLQGWVNRRRDHGELVFIDLRDR
                     SGLAQVVFDAEFLPDAAVLALAKELRSEFVVDVTGAVRTRGADAVNADLPTGGIEVRA
                     TALTILNRADTPPFPIEDGIKAAEDLRLKYRYLDLRRPEMTQRFVARSAVTMAIREAL
                     HEEGFLDVETPILTKSTPEGARDFLVPSRIHEGEFYALPQSPQLFKQLLMVSGMERYY
                     QIARCFRDEDLRADRQLEFTQVDIEMSFPTEETVYALIEKVFTRVFPKWGIACEAPFR
                     RMTYDEAMERFGIDRPDTRFGLELGNFQEIEEKISSKVERVKGLCVPGGGSFSRKRLD
                     DIEKGARAAGVEWFFWVKTAGPGSWNSNSKKFFDDAAIERLRASFGAGESDLVFLGAG
                     KAKNVLDFLGGLRLQIAREEKLIPEGRHDFLWVTDFPLLEWHEEDARWYATHHPFTSP
                     REEDLPFLENEPGRVKARAYDVVLNGMELGGGSIRIHRPEVQSRIFARLGIGPEEARA
                     KFGFLLDAFRFGAPPHGGIALGLDRLLMILTGSASIRDVIAFPKTASGTDLMTDCPNP
                     VAPEQLAELHIALAKPLKTSG"
     gene            complement(2535..>3369)
                     /locus_tag="PLB01_18605"
     CDS             complement(2535..>3369)
                     /locus_tag="PLB01_18605"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQQ79366.1"
                     /translation="VMLFGCRGRLGFTGLSVSLNSVGTEEERPGFIQALRAWFSSKVD
                     LLDADDRRRLEENPLRVLDSKNPKLAEWLADPSLPRMVDHLSQQSSEHHAELLRFLDA
                     ARIPYRIEPRLVRGLDYYTRTVFEVTAAGLGAQNALLGGGRYDKLVSDLGGPKTPGIG
                     FAIGEDRLVDVLPSEFRQAAVAAVRGVTLVPLDRGDLGDALALAGELRRAGIAVDLVT
                     AAKGPGPGLKVAERTSAAFALLLGEKERAAGTVVLKVLATRSQEELPRGSVVETLRAR
                     L"
BASE COUNT          543 a         1147 c         1185 g          494 t
ORIGIN      
        1 gcacgccgga cgtctcgaac gggtccgcgg acacgacggc ggccttgacg cggagactgc
       61 ggaatacggc ctcggcgacg gcggcgccct cgcgagaggc gagcggtccc gcgaggcggg
      121 ccatcgcgcg gtggaatcgc gcatcgagga aggacgtctt cacgacctcg gcgaggccgg
      181 accggaacgc ccgctcgggc agcgtgtcga ggacggcggt gtccgcgagg acgaggcgcg
      241 gcgcgtggaa cgcgccggcg aggttcttgc cctgcggcag gtcgaccgcc gtcttgcccc
      301 cgagcgcgct gtcgcaggcc gcgaggagcg tcgtcggcgc ctgcacgacg ggcaccccgc
      361 gcagggcgag cgcggcggcc atcccgccca cgtcccccac cgtaccgccg ccgaggcaga
      421 ccactccccc gtcgcggcgc agaccgcggc ggacgagctg ctcgatgagg cgaccgagga
      481 cggaccacgt cttggccttc tcgccatcgg ggacgaagag gggttctttg cttaagaccc
      541 tctcagcgcc tccagctgac agcagatggc catgcaggag aaagattctc ttagaagaga
      601 caagaaacca gcggcccggt gcgatggacg ggaggcgtgc gccgagagag gccagcgccc
      661 cctcccccac gacgacgtct gtcctcgccc ccgccgcaac gaatgagaat tctttcttca
      721 ccttctaaga aaatcttctc tcttctcttc ttctcttctc ttaaccagag gtcttcagcg
      781 gcttcgcgag cgcgatgtgg agctcggcga gctgctcggg cgcgacgggg ttcgggcagt
      841 ccgtcatgag gtcggtgccg gaggccgtct tcgggaacgc gatgacgtcc cggatggacg
      901 ccgaacccgt caggatcatg agcagccggt cgaggccgag cgcgatcccg ccgtgcggcg
      961 gcgcgccgaa gcggaaggcg tcgaggagga agccgaactt cgcgcgcgcc tcctccggcc
     1021 cgatcccgag cctcgcgaag atgcgcgact ggacctcggg ccggtggatc cggatggacc
     1081 ctccgcccag ctccatgccg ttcaggacca cgtcgtaggc gcgcgccttc acgcgccccg
     1141 gctcgttttc gaggaaggga aggtcctcct cgcgcggcga cgtgaacggg tgatgcgtcg
     1201 cgtaccagcg cgcgtcctcc tcgtgccact cgaggagcgg gaagtccgtg acccagagga
     1261 agtcgtggcg cccctcgggg atcagcttct cctcgcgggc gatctggaga cggaggccgc
     1321 cgaggaaatc caggacgttc ttggcctttc cggcgccaag aaagacgaga tcggattcgc
     1381 ccgcgccgaa cgaggcgcgc agccgctcga tcgcagcgtc gtcaaagaat ttcttggagt
     1441 tggaattcca gctgcccgga ccggccgtct tcacccagaa gaaccactcg acacctgccg
     1501 cccgcgcccc cttctcgatg tcgtcgaggc gcttgcggga gaacgagcct cccccgggca
     1561 cgcacaggcc cttgactctt tcaaccttcg aagaaatctt ctcttcgatt tcctggaagt
     1621 ttcccagttc cagcccgaag cgcgtgtccg gccggtcgat gccgaagcgc tccatggcct
     1681 cgtcgtacgt catccgccgg aacggggcct cgcacgcgat cccccacttc gggaagacgc
     1741 gcgtgaagac cttctcgatg agggcgtaga cggtctcctc cgtcgggaac gacatctcga
     1801 tgtcgacctg cgtgaactcg agctggcggt cggcgcgcag gtcctcgtcg cggaagcagc
     1861 gcgcgatctg gtagtagcgc tccatccccg acaccatgag gagctgcttg aagagctgcg
     1921 gcgactgcgg cagggcgtag aactcgccct cgtggatgcg cgacgggacc aggaagtccc
     1981 gcgccccctc cggggtggac ttcgtgagga tcggcgtctc gacgtcgaga aagccctcct
     2041 cgtgcagagc ctcgcggatc gccatcgtca cggcgctccg ggcgacgaag cgctgcgtca
     2101 tctccgggcg gcgcaggtcg aggtagcggt acttcagccg caggtcctcg gcggccttga
     2161 tcccatcctc gatcgggaag ggcggcgtgt ccgcgcggtt caggatcgtg agcgcggtcg
     2221 cccgcacctc gatccctccg gtcggaaggt cggcgttcac ggcgtcggct ccgcgggtcc
     2281 tgacggcccc cgtgacgtcg acgacgaact cgctccggag ctccttggcg agcgcgagga
     2341 cggcggcgtc gggcaggaat tcggcgtcga agacgacctg cgcgaggccg gagcggtccc
     2401 gaaggtcgat gaagacgagc tcgccgtggt cgcggcggcg gttgacccag ccctgcaggc
     2461 ggaccgtctg gcccgcgtgg gacgggcgca gggagccggc gggcgtgcga gagagagcgg
     2521 acatggccgg gatcttacag acgggcccgc agcgtctcca cgaccgaccc gcgcggcagc
     2581 tcttcctgag aacgcgtcgc gagcaccttg aggacgacgg tgccggcggc gcgctccttc
     2641 tcgccgagga gcagggcgaa ggccgcactc gtgcgctcgg cgaccttgag gccggggccg
     2701 gggcccttgg ccgccgtcac gaggtccacg gcgatgcccg cgcgccggag ctcccccgcg
     2761 agggcgagcg cgtcgccgag atcgcccctg tcgagcggga cgagcgtcac cccgcgcacc
     2821 gccgcgacgg ccgcctgccg gaactcgctc gggaggacgt ccacgaggcg gtcctcgccg
     2881 atcgcaaagc cgatcccggg cgtcttcgga ccgccgaggt cggagacgag cttgtcgtac
     2941 cgcccgccgc cgaggagcgc gttctgcgcg ccgaggccgg cggcggtgac ctcgaagacg
     3001 gtgcgggtgt agtaatcgag gccccggacg agacggggct cgattcggta cggaatccgc
     3061 gccgcgtcga gaaaacgaag gagctccgcg tgatgctcgg aagattgctg agaaaggtga
     3121 tcgaccatgc gcggcagcga ggggtcggcg agccattccg cgagcttcgg gttcttcgaa
     3181 tcgaggacgc ggagggggtt ttcctcgagg cgccgccggt cgtctgcgtc gagcagatcc
     3241 accttcgaag aaaaccaggc gcgcagcgcc tggatgaacc ccggtctctc ttcctcggtc
     3301 ccgaccgagt tgagcgacac cgagagcccc gtgaagccga ggcgcccgcg gcagccgaag
     3361 agcatgacg
//
LOCUS       DAOMFY010000363         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11746_length_3338_cov_7.443497, whole genome shotgun sequence.
ACCESSION   DAOMFY010000363 DAOMFY010000000
VERSION     DAOMFY010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_3338_cov_7.443497"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="PLB01_18610"
     CDS             <1..1914
                     /locus_tag="PLB01_18610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ79367.1"
                     /translation="AAFVPLALYAAALVLTRRGILLLEPYFHPERDVPLLAGFGLALA
                     AAIWDRVATGRKDPYWEAVLTAPMEDRRLLLALLGGSLALAGGMALLYRWRAAKLETD
                     SASLWAALHVLVFGMVAASFRGRVRLRETFRTHWVVLVPLAAACVTHFYRLLDVPTNV
                     HFDFVYCSVSALELMDGRLASIFQTGFVPVPVVGLLPEMAGFLLAGPTELGFRLGPAL
                     AGISGVLAVYVLGTTWRSRAAGLLGAIFLAGAVPWIHFSRMSTTGVSAVAGLWLLVFF
                     ALALKTNRAGWWLLGGWVAGWCFYLWPASRVAPVACFLAGLVLALRSPRATARRWAGP
                     PLMALVFLVWLVPLLPAWVASPNLALPRAQESLEVYKPQGGLDHDRLRASFGRPLAMS
                     AGWFFTTPDQSSQGSMSPALNTAEAMLFTLGVAVALAAGFSLNVLLLLHVALVLLVLG
                     AFAGSPPWYTRMLPSLPVACVFMGAAVAALLESLRLPNVRWRAAVLAAAAAAALALSP
                     AANLRRYVEYEGERRTMWEATALGRRLRTIGPGRTVYIVTTGRSDWSLNVNGERPARL
                     GEMLPLVWNLHLVEIRELEEAGALAAGPKAIVVPKERIAADLPRLLAAWPSARVEDLP
                     GVDGPWARILLID"
     gene            complement(1927..2451)
                     /locus_tag="PLB01_18615"
     CDS             complement(1927..2451)
                     /locus_tag="PLB01_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79368.1"
                     /translation="MKLTRTNVWVLACAAVLVLSACGGKKEAPKPAAGSAAAAATPAA
                     EAAAPAGAASGKAQMHIDAYRLGYAFGPDGQVRGEGNTFAKGDKIFLSYGIRDAKAGS
                     STKVVWVKSPAGTKLSEESKPLPPDPGTVGFVADTASWSNGEYAVEIWVVEPSAEPRR
                     LGAATLTVGASRGK"
     gene            complement(2558..>3338)
                     /locus_tag="PLB01_18620"
     CDS             complement(2558..>3338)
                     /locus_tag="PLB01_18620"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HQQ79369.1"
                     /translation="AGAPRPARWSAALRVPRYGNVLFSIAAGVLAVDGREILRARAGA
                     REVLVALPEGDHRVELVAPLGGGPATFLWQPEGERALRRTRVTELQAVDGPAQGLLGT
                     YNHPKAAVQLRLDGTLAAMSLGSDFGLDDAWTAHWSGTLLAPADGAYTFGFFTHGGTV
                     EMSLDGGRPLRTDGDGEKLSRFPPVTLARGRHAVEITYRIVHRPAAIDWVWTPPGGEE
                     SVVPPSVLRPPAGAGPRPPLSGADLAALRERRRGTPFLFVP"
BASE COUNT          369 a         1273 c         1180 g          516 t
ORIGIN      
        1 gccgcgttcg tgccgctcgc gctctacgcc gcggccctcg tcctcacacg gcgcggcatt
       61 ctcctcctcg agccgtactt tcacccggag cgcgacgtgc cgctcctcgc cggcttcggc
      121 ctcgcgctcg ccgccgccat ctgggaccgg gtcgcgaccg ggcgcaagga cccttactgg
      181 gaggccgttc tcacggcgcc catggaggac cggcgcctgc tcctcgcgct gctcgggggc
      241 agcctcgcgc tcgccggggg aatggcgctg ctctaccgct ggcgcgccgc caagctcgag
      301 acggactcgg cgtcgctctg ggccgccctc cacgtcctcg tcttcggcat ggtggcggct
      361 tctttccgcg ggcgggtccg cctgcgcgag acgttccgca cgcattgggt cgtcctcgtg
      421 ccgctcgccg ccgcatgtgt gacccacttc taccggctcc tcgacgtccc gacgaacgtc
      481 cacttcgact tcgtctactg ctcggtctcc gccctcgagc tgatggacgg ccggctcgcg
      541 agcatcttcc agacgggctt cgtccccgtg cccgtcgtcg gcctcctccc cgagatggcc
      601 ggcttcctcc tcgcgggccc caccgagctc gggttccggc tcggccccgc cctcgccggc
      661 atctccggag tcctcgcggt gtacgtcctc ggcaccacgt ggcgctcccg ggccgccgga
      721 ctcctcggcg cgatcttcct cgcgggcgcg gtgccctgga tccacttcag tcgcatgtcc
      781 accacgggcg tctccgcggt cgcgggactc tggctgctcg tcttcttcgc gctcgcgctg
      841 aagacgaacc gcgcgggctg gtggctcctc ggcggatggg tcgcgggctg gtgcttctac
      901 ctctggccgg cctcgcgcgt cgcgcccgtc gcttgcttcc tcgcggggct cgtcctcgcc
      961 ctccggtcgc cgcgcgcgac cgcccgccgc tgggccgggc cgccgctcat ggcgctcgtc
     1021 ttcctcgtgt ggctcgttcc gctccttccc gcgtgggtgg cgtcgccgaa cctcgcgctg
     1081 ccgcgcgcgc aggagtcgct cgaggtctac aagccccagg gcggcctcga ccacgatcgg
     1141 ctgcgcgcgt ccttcggaag gccgctcgcg atgagtgcgg gctggttctt cacgacgccg
     1201 gaccagagca gccagggctc gatgtcgccc gcactgaaca cggccgaggc gatgctgttc
     1261 acgctcggcg tcgcggtcgc gctcgccgcc gggttctcgc tgaacgtcct cctgctcctc
     1321 cacgtcgcgc tcgtcctgct cgtgctcggc gcgttcgcgg gctcgccgcc gtggtacacg
     1381 cgaatgctcc cgagcctgcc ggtcgcgtgc gtgttcatgg gagcggccgt cgccgctctg
     1441 ctcgaaagcc tgcgccttcc gaacgtgcga tggcgcgcgg ccgtcctcgc cgccgccgcc
     1501 gccgcggctc tcgcgctctc ccccgccgcc aacctgcgcc gctacgtcga gtacgagggc
     1561 gagcggcgca cgatgtggga ggcgacggcg ctcggccgca ggctccggac gatcggcccc
     1621 ggccgaaccg tctacatcgt cacgacgggg cgctcggact ggtcgctcaa cgtgaacggc
     1681 gagcgtccgg cgcgcctcgg ggagatgctg ccgctcgtct ggaacctcca cctcgtcgag
     1741 atccgcgagc tggaggaggc gggcgcgctc gccgccggtc cgaaggcgat cgtcgtaccg
     1801 aaggagcgca tcgcggcgga cctcccgcgt ctcctcgcgg cgtggccgtc ggcgcgcgtg
     1861 gaggacctgc cgggcgtcga cggcccgtgg gcgcggatcc tcctgatcga ctgaaggggc
     1921 ggcgggctac ttgccgcggc tcgcgccgac cgtgagcgtc gccgcgccga ggcggcgcgg
     1981 ctcggccgag ggctcgacga cccagatctc gacggcgtac tcgccgttcg accagctcgc
     2041 cgtgtccgcg acgaagccga ccgtccccgg gtcgggaggc agcggcttcg actcctcgct
     2101 cagcttcgtg ccggccgggc tcttcaccca cacgaccttc gtggacgagc cggccttcgc
     2161 gtcccggatg ccgtaggaga gaaagatctt gtcgcccttg gcgaacgtgt tgccttcgcc
     2221 gcgcacctgg ccgtcgggcc cgaatgcgta accgagacgg tacgcgtcga tgtgcatctg
     2281 cgccttgccg gaggcggccc cggcgggcgc tgcggcctcc gcggccggag tcgcggcggc
     2341 ggccgcactg cccgcggcgg gcttgggcgc ttctttcttg ccgccgcagg ccgaaagcac
     2401 gaggaccgcg gcgcaggcca ggacccagac gttcgttcgc gtgagcttca tgtccaagac
     2461 tccgtccggc aggttgtacc cggaccggaa ggaccggtcg ggagttcgcg ccggcagtgg
     2521 ccgaagaata gcgcccccgc ctccggattt ccaacgctca tggcacgaag aggaacggcg
     2581 tgccgcgccg cctctcgcgc agcgccgcga gatcggctcc cgagaggggc ggccgcggcc
     2641 ccgcgccggc gggaggccgg aggacggacg gcgggacgac ggactcttcc ccgccgggag
     2701 gcgtccagac ccagtcgatc gccgccggcc tgtggacgat ccggtaggtg atctccaccg
     2761 cgtgccggcc gcgcgcgagg gtcacgggcg gaaagcggct gagcttctcg ccgtcgccgt
     2821 ccgtgcgaag agggcggccg ccgtcgagcg acatctcgac cgtcccgccg tgcgtgaaga
     2881 aaccgaacgt gtacgcgccg tccgcgggcg cgaggagcgt gccgctccag tgcgcggtcc
     2941 acgcgtcgtc gaggccgaaa tccgagccga gcgacatcgc cgcgagggtg ccgtcgaggc
     3001 gcagctgtac cgccgccttc ggatggttgt aggttccgag caggccctgc gcggggccgt
     3061 ccacggcctg gagctcggtc acacgcgtcc gccggagagc gcgctctccc tccggctgcc
     3121 agaggaacgt cgcgggaccg cctccgaggg gcgcgaccag ctcgacgcgg tggtcccctt
     3181 ccggcagcgc gaccaggact tcgcgagctc cggcgcgcgc gcggaggatc tcgcggccgt
     3241 ccacggcgag gacgccggcc gcgatcgaga agaggacgtt tccgtagcgc ggcacgcgca
     3301 gggccgcgct ccagcgcgcc gggcgcggcg cgccggcg
//
LOCUS       DAOMFY010000364         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11747_length_3338_cov_5.490405, whole genome shotgun sequence.
ACCESSION   DAOMFY010000364 DAOMFY010000000
VERSION     DAOMFY010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11747_length_3338_cov_5.490405"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLB01_18625"
     CDS             <1..200
                     /locus_tag="PLB01_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79370.1"
                     /translation="SCEGGGQTAWDSGELPAKSAEDGDLSLRLPPGVPPAGGYAVRLR
                     SRGPAGAPQDSFLGTLDIVER"
     gene            complement(213..863)
                     /locus_tag="PLB01_18630"
     CDS             complement(213..863)
                     /locus_tag="PLB01_18630"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ79371.1"
                     /translation="MTRPVRPESRGGGVLSRRRPPSPHVETKRRAKRARILESAVRSF
                     AAKGFYGTSMDDIAEELLLTRGSLYYYFKDKEEILALCHETALEAMLEVTGRVRASQL
                     PPDQALRRIVVEHARVMVDKFHGTALALQFDALDPKRRASVVGARDAYERSVRDVIAD
                     GIAKKVFRPVDPKLATFAILGAINWLARWYRAGGGASVDEVGESFADLFLAGLLAT"
     gene            complement(938..1014)
                     /locus_tag="PLB01_18635"
     tRNA            complement(938..1014)
                     /locus_tag="PLB01_18635"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(978..980),aa:Pro,seq:cgg)
     gene            1105..1452
                     /locus_tag="PLB01_18640"
     CDS             1105..1452
                     /locus_tag="PLB01_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQQ79372.1"
                     /translation="MEYLLLIYENEKNWESMPEAEQGAMFGEYMSFTEDIKKKGHYKG
                     GNPLQPTATATTVRVRGGKTLATDGPFAETKEQLGGYYLVEAKDLDEAIAIAARIPSA
                     RLGSIEVRPIQKM"
     gene            1498..2745
                     /locus_tag="PLB01_18645"
     CDS             1498..2745
                     /locus_tag="PLB01_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQQ79373.1"
                     /translation="MDLDRLFRDESGRILASLIRLVGDFDRAEEAMQEAFVVAAERWP
                     AEGVPASPRAWIVGTARHKALDRLRRERVFAGKAPLLREEEAVNPPAPDGLLEELPDD
                     RLRLVFTCSHPALALDARVALTLHTLGGLTTAEIARAFLVPEGTLAQRLVRAKRKIRG
                     AGIPYRVPSRAELPGRLEGVLATLYLIFNEGYAASAGEALVRRDLSREAIRLARLLVE
                     LLPGEREARGLLALMLLTDARRDARVDAAGDLVLLEDQDRRRWDQAAIAEGLALLPGA
                     LAGSPPGPYAVEAAIAALHARAATPAETDWRQIAALYGTLRRVRPSPVVALNRAVAVA
                     MSAGPEAGLPLVEELERSGALAGYHLLPAAKADLLRRLGRHFEAAESYRVALALSGNG
                     PERRFLSRRLEEAERASAARAAP"
     gene            complement(2640..>3338)
                     /locus_tag="PLB01_18650"
     CDS             complement(2640..>3338)
                     /locus_tag="PLB01_18650"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQQ79374.1"
                     /translation="GAWPRDPDQTTHFTTATADGGVVAVTYTLNDTLGNHTVVPGVGV
                     LLNNEMDDFATEPGAANTYGLVQGESNAVRAGARPLSSMVPTLVLEEGRPRLALGSPG
                     GSLIPTTVLQVALNALHRGEPLGEAVAARRFHHQHLPDRIELEQGAFPGDVMEALRAK
                     GHAVHVRGDRFTEGKIGRVHAIAFERDGSLTAAADPRGYGAARAAEARSASSSRRDRK
                     RLSGPLPESASATR"
BASE COUNT          466 a         1222 c         1206 g          444 t
ORIGIN      
        1 cgagttgcga gggcggcggc cagaccgcct gggactccgg cgagctgccc gcgaagagcg
       61 ccgaggacgg agatctctcc ctgcgcctgc cgccgggcgt gccgcccgcg ggcggctacg
      121 cggtccggct gcgttcgcgc ggccccgcgg gcgccccgca ggacagcttc ctcgggacgc
      181 tcgacatcgt cgaacgctga gcggccgagc ggctacgtcg cgagaaggcc cgcgaggaag
      241 aggtccgcga acgactcgcc gacctcgtcc accgacgccc cgccgccggc ccggtaccag
      301 cgtgcgagcc agttgatcgc gcccaggatc gcgaacgtcg cgagcttcgg gtcgacgggc
      361 cggaacacct tcttcgcgat cccgtcggcg atgacgtcgc ggacgcttcg ctcgtatgcg
      421 tcgcgggcgc ccaccacgga cgcgcggcgc ttcgggtcga gggcgtcgaa ctggagggcg
      481 agagccgtgc cgtggaactt gtcgaccatc acgcgcgcgt gctcgacgac gatccggcgg
      541 agggcctggt cgggcggaag ctgggaggcc cgcacgcggc ccgtcacctc gagcatcgcc
      601 tcgagcgccg tctcgtggca gagggcgagg atctcttcct tgtccttgaa gtagtagtag
      661 aggctgcccc gcgtcaggag gagctcttcg gcgatgtcgt ccatcgacgt cccgtagaaa
      721 cccttggccg cgaagctcct caccgcgctc tcgaggatgc gcgcgcgctt cgcccgccgc
      781 ttcgtctcga cgtgcgggga gggcgggcgg cggcgcgaga ggacgccgcc cccgcgggac
      841 tcgggacgga ccggccgcgt catggcgggg aatcttaccg agtcgccgcg cgggcatgaa
      901 aaagccccgc cttcgggagc ggggctgacg tgagaaatgg tcggggcgag aggattcgaa
      961 cctccgaccc cacggtcccg aaccgtgtgc gctaccaggc tgcgctacgc cccgaggagc
     1021 gcggacgata acacgggatg tcgatttcgg gcagtcccgt tcgaccagta ggaaaggccc
     1081 ccgagggggc gcgaggaggc aaccatggaa tacctgctgc tcatctacga gaacgagaag
     1141 aactgggaat cgatgccgga ggccgagcaa ggcgccatgt tcggcgagta catgtccttc
     1201 accgaggaca tcaagaagaa gggccattac aagggcggca acccgctcca gcccacggcc
     1261 acggcgacga cggttcgcgt gaggggcggc aagaccctcg cgacggacgg cccgttcgcc
     1321 gagacgaagg agcagctcgg cgggtactac ctcgtcgagg cgaaagacct cgacgaggcg
     1381 atcgcgatcg ccgcccggat tccgtcggcc cgcctgggct cgatcgaagt ccggcccatc
     1441 cagaagatgt aagcgggcgg caaccccggt ccacggacgg acgtatcacg ttcgcccgtg
     1501 gacctcgacc gcctcttccg cgatgagtcc ggacgcattc tcgcgagcct catccggctc
     1561 gtcggggact tcgatcgcgc cgaggaggcc atgcaggagg cgttcgtggt cgccgcggag
     1621 cgctggccgg ccgagggcgt cccggcgagt ccccgcgcat ggatcgtcgg gaccgcgcgc
     1681 cacaaggcgc tcgaccgcct gcgccgcgag cgggtcttcg cgggcaaggc gcccctcctg
     1741 cgcgaggagg aggccgtgaa cccgcccgcc ccggacggcc tgctcgaaga actccccgac
     1801 gaccgcctcc ggctcgtctt cacgtgctcg cacccggcgc tggcgctgga cgcgcgcgtc
     1861 gcgctcacac tgcacacgct cgggggcctc acgacggcgg agatcgcgcg cgcgtttctc
     1921 gttccggaag gcacgctggc ccagcgcctc gtccgcgcca agcggaagat caggggcgca
     1981 ggcatcccgt accgggtgcc ctcgcgcgcc gagctccccg ggcgcctcga gggcgtcctc
     2041 gcgacgctct acctcatctt caacgagggc tacgcggcgt ccgcgggcga ggcgctcgtg
     2101 cgccgcgacc tctcccgtga ggcgatccgg ctcgcgcgcc tcctcgtcga gctccttccc
     2161 ggcgagcgcg aagcgcgcgg cctcctcgcc ctcatgctcc tgacggacgc acgccgcgac
     2221 gcccgggtgg acgccgcggg cgatctcgtc ctcctggagg accaggaccg gcgccgatgg
     2281 gaccaagcgg cgatcgccga aggcctcgcg ctcctgccgg gggccctcgc gggctcgccg
     2341 ccggggccgt acgccgtcga ggcggccatc gcggcgctcc acgcgcgcgc cgcgacgccc
     2401 gccgagacgg actggcggca gatcgccgcg ctctacggaa cgctccgccg cgtgcgccct
     2461 tcccccgtcg tcgcgctcaa ccgcgcggtc gcggtcgcga tgtcggccgg tcccgaagcg
     2521 gggctcccgc tcgtcgagga gctcgagcgc tccggcgccc tcgcgggcta ccacctgctt
     2581 cccgcggcga aggccgacct cctgcgccgg ctgggccggc acttcgaggc cgcggaaagc
     2641 taccgcgtgg cgctcgcgct ctccggcaac ggcccggaga ggcgtttcct gtcccggcgg
     2701 ctcgaggaag cggagcgcgc ctccgctgct cgcgccgcgc cgtaaccgcg cgggtcggcg
     2761 gcggccgtga gcgagccgtc cctctcgaac gcgatcgcgt ggacgcggcc gatcttccct
     2821 tccgtgaacc tgtcgccgcg cacgtggacc gcgtggccct tcgcgcggag cgcctccatc
     2881 acgtccccgg gaaacgcgcc ctgctcgagc tcgatgcggt ccggaaggtg ctggtggtgg
     2941 aagcgccgcg ccgcgaccgc ctcgccgagc ggctcgccgc gatggagggc gttcagggcc
     3001 acctgcagga ccgtcgtcgg gatgagggaa cccccggggg aaccgagcgc gaggcgcggg
     3061 cggccctcct ccaggacgag cgtcggcacc atggaggaga gcggacgcgc cccggcacgc
     3121 accgcgttcg actcgccctg gacgagtccg taggtgttcg cggcgccggg ctcggtcgcg
     3181 aagtcgtcca tctcgttgtt caggaggacg ccgacgccgg gcacgaccgt gtggttcccg
     3241 agcgtgtcgt tcagcgtgta cgtgacggcg acgaccccgc cgtcggccgt cgccgtcgtg
     3301 aagtgcgtcg tctggtccgg gtcgcgcggc cacgcgcc
//
LOCUS       DAOMFY010000365         3321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11850_length_3321_cov_6.236375, whole genome shotgun sequence.
ACCESSION   DAOMFY010000365 DAOMFY010000000
VERSION     DAOMFY010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11850_length_3321_cov_6.236375"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLB01_18655"
     CDS             complement(<1..726)
                     /locus_tag="PLB01_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79375.1"
                     /translation="MRLRLVTHAGPLDVEGDHVADAAGEPELSLDLDGATLRPGLVNA
                     HDHLHRNHLPRLGEPPYADAATWGRDLHERFADGLDLRHALPRRDALLFGALKNVLGA
                     ATTAVHHDAWEEDFGHDFPVRVPRIPTAQSVREAPAVAPPGPWTMHLAEGTSRGAREE
                     IADADARGLLGRSLAAVHLVATDADGLARLTRAGCAAVWCPTSNAFLYGATASRALFA
                     AGLEVLLGTDALVSGAGTLLDELA"
     gene            complement(723..1400)
                     /locus_tag="PLB01_18660"
     CDS             complement(723..1400)
                     /locus_tag="PLB01_18660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ79376.1"
                     /translation="MSAVLKTREAYRLWSDTYDEENPLTVLDEAAARILTPELEGLDL
                     LDAACGTGRRLVFRENPPRSAAGIDLVFEMLARGRRVPGRPSSTSAADVRSLPFPAAR
                     FDVAWCRLAAGHLPALAPLYKELARVLRPGGCAVVTDFHPEAIRRGHARIFRDGAGAS
                     HAVEHVVHEPGDHETAARAAGLAFDSCGWLGVGSEVRSFYERAGKLDRYARDAGMPLL
                     LALRFSR"
     gene            complement(1397..2764)
                     /locus_tag="PLB01_18665"
     CDS             complement(1397..2764)
                     /locus_tag="PLB01_18665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ79377.1"
                     /translation="MILLLNTRATKAKNRRFPLSLLALAAALPEGVTWEIVDGNLEGS
                     DPAADAAAIVAAREGTDDPVTLVGMTVMPGPQLLSAVPVARALKDRFPHLPIVWGGYF
                     PSLYPAPVLRAPYVDYIVRGQGERTLVDLLGVLAGRVDPASVAGLGWKDAGQARLNPE
                     RPWEGPDAFPRVPWPRVDVPTYLRPSALGARSGVYQASIGCPYTCNFCGVIGAFGSRE
                     KFATPARTETDLAHLAATHGMDSVHFYDNNFFLREEHAVELCERIAPLGLKWWAEARI
                     DAMLRFSDATWRTIRRAGLTMAYFGAESGSNERLRKMSKNLTVEQTLALAAKTKEHGI
                     LPEFSFVFGDPDDPEDDVETNLAFIEKLAAVNPDLKLITYFYTPTPQRRGTYGNVDPL
                     STTPATLEEWTTPEWLGWMTHEDPRVPWLRARLKGRVEDFARRFKGGLVLADSQAYGH
                     LREAS"
     gene            complement(2761..>3321)
                     /locus_tag="PLB01_18670"
     CDS             complement(2761..>3321)
                     /locus_tag="PLB01_18670"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ79378.1"
                     /translation="LAERGHRCVAADLRVDDVDGLGAAAPFARRLSRMFGRVAASFDA
                     LPVRPRLFDLVVFDASLHLAEDLARVLAEAARAVAPGGRVAVLDSPFYARAASGEAMR
                     AEKRRETAARFADAAEDLLALSPVEFLTKERLASAAAPAGLEFRRRRVLYPAAYEARR
                     VVARLKGARAPSRFDLWVARAPGGAA"
BASE COUNT          468 a         1161 c         1285 g          407 t
ORIGIN      
        1 ggcgagctcg tcgagcagcg ttccggcgcc gctcacgagc gcgtccgttc ccagcagcac
       61 ctcgagaccg gcggcgaaga gggcgcgcga ggccgtcgcg ccgtacagga acgcgttgga
      121 cgtcgggcac caaacggccg cgcaccccgc gcgcgtcagg cgggccagtc cgtcggcgtc
      181 cgtcgcgacg aggtggaccg ccgcgagcga ccgcccgagg aggccccggg cgtccgcgtc
      241 cgcgatctcc tcgcgcgccc cgcggctcgt cccttcggcg aggtgcatcg tccacggccc
      301 cgggggagcc accgcgggcg cctcgcgcac ggactgcgcc gtcggaatgc ggggtacgcg
      361 gaccgggaag tcgtggccga agtcctcctc ccacgcgtcg tggtggacgg ccgtggtcgc
      421 ggcaccgagg acgttcttga gcgccccgaa caggagcgcg tcgcggcgcg ggagcgcgtg
      481 ccggagatcg aggccgtccg cgaaccgttc gtggaggtcg cgaccccatg tcgccgcgtc
      541 ggcatagggc ggctctccga ggcgcggcag gtgattgcgg tggaggtggt cgtgcgcgtt
      601 cacgaggccg ggccggagcg tcgccccgtc gaggtcgagg ctgagctccg gctcgccggc
      661 ggcgtccgcc acgtgatcgc cctcgacgtc gagggggccg gcgtgcgtga cgaggcgcag
      721 cctcaccgcg agaacctcag cgcgaggagg agcggcatcc cggcgtcgcg cgcgtagcgg
      781 tcgagcttcc cggcccgctc gtagaaggag cgcacctccg acccgacgcc cagccagccg
      841 caggagtcga acgcgaggcc ggcggcgcgc gccgccgtct cgtggtcgcc cggctcgtgc
      901 acgacgtgct cgacggcgtg cgacgcgcct gcgccgtcgc ggaagatgcg cgcatgcccg
      961 cggcgaatcg cctcggggtg gaagtccgtg acgaccgcgc acccgcctgg cctgaggacg
     1021 cgcgcgagct ccttgtagag cggggcgagc gcgggaaggt gcccggccgc gagacggcac
     1081 caggcgacgt cgaaccgtgc ggcgggaaac ggcagggacc tcacgtccgc ggccgaggtg
     1141 gacgacgggc ggccgggcac acgccggccc cgggcgagca tctcgaagac gaggtcgatc
     1201 ccggccgccg agcgaggcgg gttctcgcgg aaaacgaggc ggcggccggt gccgcaagcc
     1261 gcgtcgagga ggtcgagccc ctcgagctcc ggcgtgagga tccgcgcggc cgcctcgtcg
     1321 agcacggtca acgggttctc ctcgtcgtac gtgtccgacc agaggcggta cgcctcgcgc
     1381 gtcttcagga cggcgctcac gaggcctccc tgaggtggcc gtaggcctgc gagtccgcga
     1441 ggacgaggcc gcccttgaag cggcgcgcga agtcctcgac gcgccccttc agccgcgcgc
     1501 gcagccaggg gacccgcggg tcctcgtgcg tcatccagcc gagccactcg ggcgtcgtcc
     1561 attcctcgag cgtcgcgggc gtcgtcgaca gcgggtcgac gttcccgtac gtcccgcggc
     1621 gctgcggggt cggcgtgtag aagtacgtga tgagcttgag gtccgggttg accgcggcga
     1681 gcttctcgat gaacgcgagg ttcgtctcga cgtcgtcctc ggggtcgtcg ggatcgccga
     1741 agacgaacga gaactccggc aggatcccgt gctctttcgt cttcgcggcg agcgcgagcg
     1801 tctgctcgac cgtcaggttc ttcgacatct tcctgaggcg ctcgttcgag ccggactccg
     1861 ccccgaagta cgccatcgtg aggcccgcgc gccggatcgt ccgccacgtc gcgtcggaga
     1921 agcgcagcat ggcgtcgatc cgcgcctcgg cccaccactt caggcccagc ggcgcgatcc
     1981 gttcgcagag ctccacggcg tgctcctcgc ggaggaagaa gttgttgtcg tagaagtgga
     2041 cggagtccat cccgtgcgtc gcggcgaggt gcgcgaggtc ggtctcggtg cgcgcgggcg
     2101 tcgcgaactt ctcccggctg ccgaacgcac cgatgacgcc gcagaaattg caagtgtacg
     2161 gacacccgat cgacgcctgg tagacgccgc tccgcgcacc gagcgccgac gggcgcaggt
     2221 acgtcgggac gtcgacgcgc ggccatggaa cgcgcgggaa cgcgtcgggc ccctcccacg
     2281 gtcgttccgg gttgaggcgc gcctggccgg cgtccttcca gccgaggccc gcgacggacg
     2341 cggggtcgac gcgtccggcg aggacgccga gcaggtcgac gagcgtgcgc tcgccctggc
     2401 cgcggacgat gtagtccacg tacggggcgc gcaggaccgg cgcggggtag aggctcggga
     2461 aatagccgcc ccagacgatc gggagatgcg gaaagcggtc cttcaacgct cgcgcgacgg
     2521 gcacggcgga gaggagctgc ggccccggca tcacggtcat gccgacgagc gtgacgggat
     2581 cgtccgttcc ttcgcgtgcg gcgacgatcg cggcggcgtc ggcagccggg tcggatcctt
     2641 cgaggttgcc gtcgacgatc tcccacgtga cgccctcggg cagcgccgcg gcgagcgcga
     2701 ggagcgagag cggaaaccgc cggttcttcg ccttcgtggc gcgcgtgttc aggaggagga
     2761 tcacgcggcg cccccgggcg cgcgcgcgac ccagagatcg aagcgcgacg gcgcgcgggc
     2821 gcccttgagg cgcgcgacga cacggcgcgc ctcgtaggct gcgggataga ggacgcgtcg
     2881 gcgccggaac tcgaggcccg cgggtgcggc ggccgaggcg agacgctcct tcgtgaggaa
     2941 ctcgaccggg gagagtgcga gcaggtcctc cgcggcgtcc gcgaagcgcg cggcggtctc
     3001 gcgcctcttc tcggcgcgca tcgcctcacc cgacgcggcg cgcgcgtaga acggcgaatc
     3061 gagcacggcg acgcgcccgc cgggcgccac ggcgcgcgcc gcttcggcga gaacgcgcgc
     3121 gaggtcctcc gcgaggtgca gcgacgcgtc gaagacgacg aggtcgaaga ggcgcgggcg
     3181 gacgggaaga gcgtcgaacg aggcggcgac gcggccgaac atccgggaaa ggcgccgcgc
     3241 gaacggcgcc gcagccccga gcccgtcgac gtcgtcgacg cgaaggtccg cggcgacgca
     3301 gcggtggccg cgctcggcga g
//
LOCUS       DAOMFY010000366         3314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_11892_length_3314_cov_5.709113, whole genome shotgun sequence.
ACCESSION   DAOMFY010000366 DAOMFY010000000
VERSION     DAOMFY010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11892_length_3314_cov_5.709113"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PLB01_18675"
     CDS             <1..1050
                     /locus_tag="PLB01_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79379.1"
                     /translation="LAQGARRAEDGRAPSSTHVAGLWGVNVVSAGDGGTFRYDGEAYR
                     RAMALAPPADLAERVRLRLLTACGSITDGRGVFDPKAASRREKDLGEFLASFPASPRR
                     TALQLERARLLTSLAENAIRTGDSESFPIYRDAAIESASEVSSTAADASRRRAADRLV
                     ARLTKSLPKRVVSEKPVVSAGGLRAQFVSKNGATLLVVTRPDGKDAIQPFTAVGADPA
                     SLAFDASGRRLVWDEAPVSGRRRTRLLDLARARVFDPAAAADPEILAAGGAGAADAGN
                     ADRYTTSLGFSPDGRLLLVVCEGFTADGTRIPKRHVLCDVEGGRRPIFVDRPFSAPGV
                     VDWPRLAQTGDRLSG"
     gene            1060..1536
                     /locus_tag="PLB01_18680"
     CDS             1060..1536
                     /locus_tag="PLB01_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79380.1"
                     /translation="MTKSHEVEDRRLRRKTAAFRLKHDVGKALRWSAPEEPETDPAAL
                     RARLAADLLPRATDAGAKRDVLKSFFEWKRDEGSMFDSSDADLQALEEAMATVARLAP
                     DLLSLKEISLSSLQSLDSACLEASRACTAFYRRVAVEEGPEVLRPLGGRGRRGGGS"
     gene            1533..3182
                     /locus_tag="PLB01_18685"
     CDS             1533..3182
                     /locus_tag="PLB01_18685"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQQ79381.1"
                     /translation="MKGGPRLLVVDDQPRTAELFRKFAPDLVLAGPSDSKAFAASWAE
                     AEAALARGGRLPDAVVLDLRFEIADEDLLPDKKPLGDSAAGKRERRERRDRQGLYILE
                     RLRRRAPDLPVVLTTAHEDIPFEDEARRLRADAFTYTVGEDETTGEGLVRVVRRVLAD
                     RDAPLSTGRFFWGRGPAMRELRRRIASLAPTPLPLLVTGPTGSGKNFLVREVLHPLSG
                     RKGPLVSFDCATVPETLLQAALFGSVRGSYTGSVADRPGVFEAAAGGTLFLDEVENLS
                     LDAQKSLLTALNDGVIRRVGATASVPHTARIVAASNVDLRKKVSDGTFRADLLMRLNP
                     ALALALPPLSERTEDLEDLADDHAAAFFSEPGHRAEILAQVRAAGAPEPDKGDAFELA
                     RGDEELSRSRAPVVFFLPRKAWAAMAKHPWPGNLRQFGMVLSDLLAATLYGRSAASLD
                     RAGRVVFAFENRLLFDLLAEARQEEADPGREDVLTLPRPKVTNVEDYRRELERAAFRQ
                     LFHECHGDFARMAERMTGSAADERAVRLRFNKLGLSARQEG"
BASE COUNT          419 a         1223 c         1207 g          465 t
ORIGIN      
        1 ctcgcgcagg gcgcccgccg cgccgaggac ggccgcgcgc cgtcgtcgac gcacgtggcc
       61 gggttgtggg gcgtgaacgt cgtctccgcc ggagacggcg gcaccttccg gtacgacggc
      121 gaggcctacc gccgggccat ggcgctcgcg cctccggcgg atctcgccga acgggtgcgc
      181 ctgcgcctcc tgacggcctg cggctcgatc acggacggac gcggcgtctt cgacccgaag
      241 gcggcctcgc ggcgcgagaa ggacctcggg gagttcctcg cgtcgttccc ggcctcgccc
      301 cggcggacgg cgctgcagct cgagcgggcg cggctcctga cgtcgctcgc cgagaacgcg
      361 atccggacgg gcgactcgga gagttttccg atctaccgcg acgcggccat cgagtccgcc
      421 tccgaggtct cgtcaacggc cgccgacgcg tcgcgccgcc gcgcggccga ccgtctcgtc
      481 gcgcgcctca cgaagtcgct cccgaagcgg gtcgtttccg agaagcccgt cgtctcggcg
      541 ggcggcctcc gcgcgcagtt cgtctcgaag aacggcgcga cgctcctcgt cgtcacgagg
      601 ccggacggga aggacgcgat ccagccgttc acggccgtgg gcgcggaccc ggcgagcctc
      661 gcgttcgacg cctcgggccg gaggctcgtg tgggacgagg cgcctgtctc gggccggcgc
      721 cggacgcgcc tcctcgacct cgcccgcgcg cgcgtgttcg accccgccgc ggccgcggat
      781 cccgagatcc tcgcggcggg cggcgcgggc gcggcggacg ccgggaacgc ggatcggtac
      841 acgacgtcgc tcggcttctc gccggacggc cggctgctcc tcgtcgtctg cgaggggttc
      901 acggccgacg gaacccgcat tccgaagcgc cacgtcctct gcgacgtgga gggcggcagg
      961 cggcccatct tcgtggaccg gccgttctcg gcgccgggcg tcgtggactg gccgcggctc
     1021 gcgcagacgg gcgatcggct gagcggataa tccgggacga tgacgaaatc tcacgaggtc
     1081 gaagaccgga ggctgcggcg caagaccgca gcctttcgct tgaagcacga cgtggggaag
     1141 gccctgaggt ggagcgcgcc ggaggagccc gagacggatc ctgccgcgct ccgcgcccgc
     1201 ctcgcggccg atctcctgcc tcgcgcgacg gacgcaggag cgaaacgtga cgtcctgaag
     1261 agtttcttcg agtggaagag ggacgaggga tcgatgttcg attcgagtga cgccgacctg
     1321 caggcgctgg aggaggcgat ggcgacggtc gcgcgcctcg ctcccgacct tctttcactc
     1381 aaagaaatct ctctctcttc tcttcaatcc ctggattccg catgccttga ggcgtcccga
     1441 gcctgcaccg cgttctaccg ccgcgtcgcc gtggaggagg gaccggaagt cctgcggccg
     1501 ctcggcggcc gcggccggcg cggaggcgga tcgtgaaggg cgggccgcgg cttctcgtcg
     1561 tcgacgacca gccccgcacg gctgagctgt tccggaagtt cgcgccggac ctcgtcctcg
     1621 cgggcccttc ggactcgaag gcgttcgccg cgagctgggc cgaggccgag gcggccctcg
     1681 cgcgaggcgg gcgcctgccc gacgccgtcg tcctcgacct ccgcttcgag atcgccgacg
     1741 aggatctcct gccggacaag aagccgctcg gcgactcggc cgccggcaag cgcgagcggc
     1801 gcgagcggcg ggaccgccag ggcctctaca tcctcgagcg cctgcggcga cgcgcgccgg
     1861 acctgcccgt cgtcctcacg acggcgcacg aggacatccc gttcgaggac gaggcgcgcc
     1921 gccttcgcgc cgacgcgttc acctacaccg tgggggagga cgagacgacg ggcgaggggc
     1981 tcgtccgcgt cgtgcggcgc gtcctcgccg accgcgacgc gccgctctcg acggggcggt
     2041 tcttctgggg ccgaggcccg gcgatgcgcg agctcagaag gcggatcgcg tcgctcgcgc
     2101 cgacaccgct gcccctcctc gtgacggggc cgacgggctc ggggaagaac tttctcgtgc
     2161 gcgaggtcct gcacccgctc tcgggccgca aggggccgct ggtttccttc gactgcgcga
     2221 ccgttcccga gacgctgctc caggctgcgc tcttcggctc cgtgcgcggc tcgtacacgg
     2281 gttcggtggc ggaccgcccg ggtgtcttcg aggcggccgc gggcggaacc cttttcctcg
     2341 acgaggtcga gaacctctcc ctcgacgcgc agaagtcgct cctgaccgcc ctcaacgacg
     2401 gggtgattcg ccgggtgggc gcgacggcgt ccgtgccgca tacggcccgc atcgtcgcag
     2461 cgtcgaacgt cgacctcagg aagaaggtct ccgacgggac gttccgggcc gacctcctca
     2521 tgcgcctgaa ccccgcgctc gccctcgcgc ttccgccgct ttcggagcgg acggaggatc
     2581 tcgaggacct cgccgacgac cacgcggccg cgttcttctc cgagcccggc caccgtgccg
     2641 agatcctcgc gcaggtgcgc gcggccgggg cgcccgagcc ggacaagggc gacgcgttcg
     2701 agctggcgcg cggcgacgag gagctctcgc gcagccgcgc cccggtcgtg ttcttcctcc
     2761 cgcgcaaggc ctgggccgcc atggcgaagc acccgtggcc cggcaacctc cggcagttcg
     2821 ggatggtcct ctcggacctg ctcgccgcca ctctctacgg gaggtcggcg gcgtccctcg
     2881 accgcgcggg acgcgtcgtc ttcgcgttcg agaaccggct cctgttcgac ctgctcgcag
     2941 aggcgcggca ggaagaggcc gacccgggcc gcgaggacgt cctgacgctc ccgcgcccga
     3001 aggtgacgaa cgtcgaggac taccggcggg agctcgagcg cgccgcgttc cggcagctct
     3061 tccacgagtg ccacggcgac ttcgcgcgca tggccgagcg gatgacgggc tcggccgcag
     3121 acgagagggc ggtgcgcctc cggttcaaca agctcggcct ctccgcacgg caggaaggct
     3181 gacccccgtg ctcgggggag tcctcgcgct cgcgctggcg gcggcccccg cgcccgccgg
     3241 ctgcgaggac gatgccgagc atctcgctcg ggccgcggcg gcgctcgtct cggcctcgcc
     3301 ccgggatgcc gagg
//
LOCUS       DAOMFY010000367         3276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_12126_length_3276_cov_5.539274, whole genome shotgun sequence.
ACCESSION   DAOMFY010000367 DAOMFY010000000
VERSION     DAOMFY010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12126_length_3276_cov_5.539274"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="PLB01_18690"
     CDS             <1..1583
                     /locus_tag="PLB01_18690"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HQQ79382.1"
                     /translation="VGTLDGMALGGIRDHLGGGFHRYSTDAEWLLPHFEKMLTDNAQL
                     LGVYAVAFAVTDDPLYAQVARETGDYLLREMRGTEGGFYAATDADSEGEEGKYFCWDP
                     SQIRDVLGRGDDAEYFLEWYSVKEGGNFLEESTGRGTGASVLHLSKKIPSSSESRLAP
                     LRAALLAARSKRIPPALDDKRVAGWNALAVSGFAVAARALGEPRYLEAARVGARFLLS
                     LSRLPDGVLARTWKDGEAKIPAFLEDEAFLALALLDLADAEGPGAAGLWHGEAEAAVE
                     SLRTRFRRGDAPGFTFSGTGNETLLSNGRDLFDKAVPSASGAAARALARLALVTGDHA
                     LAREARDAVDEVGGLMARSPHGMESWFFALELLLEFEDRYGKEKEREKEKEIPSSVKR
                     RGAEAIASPVRVEGVAVEPRVPRGERGALRLRISIAPGWHLQGPDGLRVEASAGAGEF
                     TFDEILLPPPSRIDDPIGDVLTGWHGTFEANISFLVSRKAKKGKQEIAVRVAHRACGE
                     GACRPDAVLSLSVPVEIV"
     gene            complement(1597..2106)
                     /locus_tag="PLB01_18695"
     CDS             complement(1597..2106)
                     /locus_tag="PLB01_18695"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQQ79383.1"
                     /translation="MFERPAVTIVFLALMAGAAPARPEEAAVPKSHPSIPAFFNGFNK
                     HSMHLLDDFYAPDVLFRDPVSEIRGVPGIRAYYAKMYENLISIRFDFDTEVGQGDEVF
                     APWVMHMRHKAIAGGKEIAVPGVSHFRFKNGKAVYHRDYFDMGAFVYEHVPVIGAGVR
                     YVKKKVAGH"
     gene            complement(2178..2936)
                     /locus_tag="PLB01_18700"
     CDS             complement(2178..2936)
                     /locus_tag="PLB01_18700"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HQQ79384.1"
                     /translation="MPKSLKSRLKAGEVVHGLLSPNVEASTLETLGLLGFSLYLLDTE
                     HGAAGPREAEDVARACEVAGMTLLVRPRGLDPKLILQYLDAGAMGIMLPGVRHPDDAR
                     RLVEAVKYPPKGMRGIAPVRANRFTLGPEKIEDYVERVNDETLVLPQIETLEAVAALD
                     DLVRVPGVDGFIIGPRDLAMAMGYPGGPARPEVEEAVDRITKTILGAGLLVGTTAQTG
                     ARAKELMAKGHRIVLHNVTALLRNGAAAYFAGVR"
     gene            complement(3043..>3276)
                     /locus_tag="PLB01_18705"
     CDS             complement(3043..>3276)
                     /locus_tag="PLB01_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQQ79385.1"
                     /translation="YFVKRKLYPNVDFYSGLIYQAMKFPVDMFPVLFAIPRMSGWLAQ
                     WNEMMADNDQKIARPRQIYLGSGERHIVPFEKR"
BASE COUNT          495 a         1090 c         1178 g          513 t
ORIGIN      
        1 ccgtcggcac gctcgacggc atggcgctcg ggggcatccg cgaccacctc ggcggcggct
       61 tccaccggta ttcgacggac gccgagtggc tcctcccgca cttcgagaag atgctgacgg
      121 acaacgccca gctcctcggc gtctacgcgg tggcgtttgc cgtgacggac gacccgctct
      181 acgcgcaggt cgcgcgcgag acgggcgact acctccttcg cgagatgcgc ggcaccgagg
      241 gcggcttcta cgccgcgacg gacgcggatt cggaggggga agaagggaag tatttctgct
      301 gggatccgag ccagattcgc gacgtcctcg gcaggggaga cgacgctgaa tatttcttag
      361 aatggtattc ggtcaaagag ggaggcaatt tcctcgaaga atcgacgggg cgcggcacgg
      421 gcgcttcggt ccttcacctt tctaagaaaa tcccctcttc ctccgaatcc cgtcttgctc
      481 ccctgcgcgc ggcgctcctc gccgcgcgca gcaagagaat tcctcccgcc ctcgacgaca
      541 agcgcgttgc cgggtggaac gcgctcgcgg tgtccggctt cgccgtcgcg gcgcgcgcgc
      601 tcggggagcc gcgctatctc gaggccgcgc gcgtcggcgc gcgcttcctc ctgagcctgt
      661 cgcgcctccc ggacggcgtc ctcgcgcgga cgtggaagga cggagaggcg aagatcccgg
      721 cgtttctcga ggacgaggcg ttcctcgcgc tcgccctcct cgacctcgcg gacgccgaag
      781 gccctggcgc cgccgggctc tggcacggtg aggcggaggc ggccgtggag tcgctccgca
      841 cgcgcttccg ccgcggggat gctccgggct tcacgttctc ggggacggga aacgagacgc
      901 tgctctcgaa cggccgcgac ctcttcgaca aggccgtccc gtcggcctcc ggcgccgcgg
      961 cgcgcgcgct cgcgcgcctc gcgctcgtga cgggcgacca tgcgctggcg cgcgaggcga
     1021 gggacgccgt tgacgaggtg ggcggactca tggcgcgttc gccgcacggc atggagtcgt
     1081 ggttcttcgc cctggagctc ctgctcgaat tcgaggaccg gtacgggaaa gagaaagaaa
     1141 gagagaaaga gaaagagatt ccttcgagtg taaagagaag aggggccgag gcgatcgctt
     1201 cgcccgtgcg cgtcgagggg gtggccgtcg agccgcgcgt gccgcgcggc gagaggggag
     1261 cgttgcggct ccgcatctcg atcgcccccg gctggcacct gcagggcccc gacgggttac
     1321 gcgtggaggc ctcggctggc gccggggaat tcaccttcga cgaaatcctc ttgccgccac
     1381 cctcgcgcat cgatgacccg atcggcgacg tgctcaccgg ctggcacggg accttcgaag
     1441 caaatatctc tttcctggtc tcccgaaagg cgaagaaagg gaagcaggag attgcggtgc
     1501 gcgtcgcgca ccgcgcgtgc ggcgagggcg cgtgccgccc ggacgcggtg ctttcgctct
     1561 ccgtcccggt cgagatcgtc tgaccgcggc cgggcgtcag tgcccggcga ccttcttctt
     1621 cacgtagcgg acgcccgcgc cgatcacggg cacgtgctcg tagacgaagg cgcccatgtc
     1681 gaagtagtcg cggtggtaca cggccttgcc gttcttgaac cggaagtgag agacgccggg
     1741 caccgcgatc tccttgccgc ccgcgatcgc cttgtggcgc atgtgcatga cccagggcgc
     1801 gaagacctcg tcgccctgcc cgacttccgt gtcgaagtcg aaccggatcg agatgaggtt
     1861 ctcgtacatc ttcgcgtagt acgcccggat cccggggacg ccccggatct ccgagaccgg
     1921 gtcgcggaag aggacgtccg gcgcatagaa gtcgtcgagc agatgcatcg agtgcttgtt
     1981 gaacccgttg aagaacgcgg ggatggacgg gtgcgatttc ggcacggcag cctcctcggg
     2041 gcgcgcgggc gcggcgccgg ccatgagcgc gaggaacacg atggtgacgg ccggtcgttc
     2101 gaacacacgg cacccctttc cggcgggcgc agggcggccg ccacgtcctc tctacgacgg
     2161 ggagaccgcg ggggatctca gcgaacgccg gcgaagtacg cggccgcgcc gttcctgagg
     2221 agcgccgtca cgttgtggag cacgatgcgg tggcccttgg ccatcagctc cttcgcgcgg
     2281 gcgccggtct gcgccgtcgt cccgacgagg agcccggcgc cgaggatcgt cttcgtgatc
     2341 cggtccacgg cctcttccac ctcgggccgg gccgggccgc cgggatagcc catcgccatc
     2401 gcgaggtcgc gcgggccgat gatgaagccg tccacgcccg gcacccggac aaggtcgtcg
     2461 agagccgcga ccgcctcgag cgtctcgatc tgcgggagga cgagcgtctc gtcgttcacg
     2521 cgctcgacgt agtcctcgat cttctcgggc ccgagcgtga accggttcgc gcgcaccggc
     2581 gcgatgccgc gcatgccctt cggggggtac ttcaccgcct cgacgaggcg cctcgcgtcg
     2641 tcggggtggc gcacgccggg cagcatgatg cccatcgccc cggcgtcgag gtactggagg
     2701 atgagcttcg ggtcgaggcc gcggggccgg acgagaagcg tcatccccgc gacctcgcac
     2761 gcgcgcgcca cgtcctcggc ctcgcgcggc ccggccgcgc cgtgctcggt gtcgaggagg
     2821 tagagggaga agccgaggag cccgagcgtc tcgagcgtcg aggcttcgac gttgggagag
     2881 aggaggccgt ggacgacctc gccggccttc agccgggact tgagcgactt cggcatgggt
     2941 tccctcgcgc ggatcgtacc ccccgaaacg ggacgccgcc gggcttcacg gccgcggcgg
     3001 cgtcgggatt cggggacgac cgggaagcgc ccggtccagc gctcagcgtt tctcgaaggg
     3061 cacgatgtga cgctcgccgg agccgaggta gatctggcgc gggcgggcga tcttctggtc
     3121 gttgtcggcc atcatctcgt tccactgggc gagccagccc gacatgcgcg ggatcgcgaa
     3181 gaggaccggg aacatgtcga cggggaactt catggcctgg tagatgaggc ccgagtagaa
     3241 gtcgacgttc gggtagagct tccgcttgac gaagta
//
LOCUS       DAOMFY010000368         3275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_12129_length_3275_cov_7.308696, whole genome shotgun sequence.
ACCESSION   DAOMFY010000368 DAOMFY010000000
VERSION     DAOMFY010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12129_length_3275_cov_7.308696"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLB01_18710"
     CDS             <1..699
                     /locus_tag="PLB01_18710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ79386.1"
                     /translation="RLPALAVACVWLAAKRMPPPVAGAGDGEAAAPAADGSLATRSFA
                     LLTASYTLQGYVGYIFVFWFYLYLVDVRKFDLLRGALYASLPWLLSIVSIPLGGAVSD
                     ALVKRLGPAWGRRIVPMAGLIGGAAFLSYGARTPDARVAAVCLALATALVLSVEGPFW
                     AAMMDAAGGRSGTAGGVMNMGSNLGGLVSPALTPVLAASIGWENALHVAAVLSVLGGL
                     LWLGVGSRSHAACP"
     gene            726..2762
                     /locus_tag="PLB01_18715"
     CDS             726..2762
                     /locus_tag="PLB01_18715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQQ79387.1"
                     /translation="MKIPRLTAAVLASLVLLAAASGPGIDLDGMDRSVAPGDDFFRYA
                     NGTWLRETEIPADRSAWGAGGMLDELTSMRTRELIEKAAASPAPAGSDERKIGDTFAT
                     FMDEAAIEAKGLAPLKGELDGAAALADRTALARALGGTLRADVDVLNNTNFYTANLLG
                     LWVAQDLDEPSKYSPFLLQGGLGMPDRDYYLDPAPRMADIRAKYQAHVEKMLTLAGVP
                     DAAARAARIFALEKKIAAAHASREDSEDVLKGNNHWARADFKAKAPGLDWDAYFGAAG
                     LGSQASFVVWQPGAVTGLAALVGSEPLDTWKEYLAFRALSRAATFLPKAFVDEQFAFY
                     GTTLTGAPKLRDRWKRGVSVTDAALGEAVGRLYVTKYFPPSEKARAEEMVRNEIAAFA
                     ARIDRLDWMAPQTKARAKAKLAVLKVGVGYPDKWTDYSGLEVVKGDALGNARRASLFR
                     TARALARLGKPVDRGEWVMNPQLVNAVNLPAMNAMNFPAAMLQPPYFDPKRPAVMDYG
                     ASGSVIGHEISHSFDDQGALFDAEGRLKNWWTKEDFEHFTASAQALIRQYNAYRPFPD
                     LAVNGKLTVSENIADVAGLAAAYDAYRISHGGKEAPAWRGVSGDQQFFIAFAQSWRTK
                     FREAALRQRIVIDSHAPAEYRADTVRNLDAWYPAFDVKPGQKLYLAPKDRVKVW"
BASE COUNT          475 a         1160 c         1147 g          493 t
ORIGIN      
        1 cgccttccgg ccctcgccgt cgcctgcgtc tggctcgcgg cgaagaggat gccgcccccg
       61 gtcgcgggcg ccggggacgg ggaagcggcg gcgccggccg cggacgggag cctcgcgacg
      121 cgctcgttcg cgctcctgac ggcgagctac acgctgcagg gctacgtcgg ctacatcttc
      181 gtgttctggt tctacctgta cctcgtggac gtgaggaagt tcgacctcct gaggggcgcc
      241 ctctacgcga gcctcccgtg gctcctctcg atcgtgtcga tcccgctcgg cggcgccgtc
      301 tcggacgcgc tcgtgaagag gctgggaccg gcctgggggc ggcgcatcgt cccgatggcc
      361 gggctcatcg gcggagccgc gttcctctcg tacggggcgc gaacgccgga cgcgcgcgtc
      421 gcggccgtct gtctcgcgct cgcgacggcg ctcgtcctct ccgtggaggg cccgttctgg
      481 gcggcgatga tggacgcggc gggcgggcga agcgggacgg ccggcggcgt gatgaacatg
      541 ggcagcaacc tcggcgggct cgtctcgccg gcgctgacgc ccgtgctcgc cgcgtcgatc
      601 ggctgggaga acgccctcca cgtggcggcc gttctctccg tgctcggcgg gctcctgtgg
      661 ctcggcgtcg ggtcccggtc ccacgcggct tgcccttgac ccgggatgtt cctatcgtcc
      721 cgtccatgaa gatcccgagg ctcaccgccg ccgttctcgc gtccctcgtc ctcctcgccg
      781 ccgcgtccgg gccgggcatc gacctcgacg ggatggaccg ctccgtcgcg cccggcgacg
      841 acttcttccg ttacgcgaac gggacgtggc tgagggagac cgagatcccg gccgatcgct
      901 ccgcgtgggg cgccggaggc atgctcgacg agctcacgtc gatgcgcaca cgcgagctga
      961 tcgagaaggc ggcggcgtcg ccggcccccg ccgggtccga cgagcgcaag atcggcgaca
     1021 ccttcgcgac gttcatggac gaggcggcga tcgaggcgaa gggcctcgcg ccgctgaagg
     1081 gcgagctcga cggcgctgcc gctctcgcgg acaggaccgc gctcgcgcgg gccctcggcg
     1141 ggactctccg cgccgacgtc gacgtcctga acaacacgaa cttctacacg gcgaatctcc
     1201 tcggcctctg ggtcgcgcag gacctcgacg agccctcgaa gtactccccg ttcctcctcc
     1261 agggcggcct cgggatgccg gaccgcgact actacctcga tcccgcgccg cgcatggcgg
     1321 acatccgggc gaagtaccag gcgcacgtcg agaagatgct gaccctcgcc ggcgtcccgg
     1381 acgccgccgc cagggccgcg aggatcttcg ccctcgaaaa gaagatcgcc gcggcgcacg
     1441 ccagccgcga ggactccgag gacgtcctca aggggaacaa ccactgggcg cgcgcggact
     1501 tcaaggcgaa ggcccccggt ctcgactggg acgcgtactt cggggccgcg gggctcggct
     1561 cacaggcctc gttcgtcgtc tggcagccgg gcgccgtcac gggcctcgcc gcgctcgtcg
     1621 ggtccgagcc gctcgacacg tggaaggagt acctggcgtt ccgggccctc tctcgcgccg
     1681 cgacgttcct cccgaaggcg ttcgtcgacg agcagttcgc gttctacggc acgacgctca
     1741 cgggcgcgcc gaagctccgc gaccgctgga agcgcggcgt ctcggtcacg gacgccgcgc
     1801 tcggcgaggc cgtcggtcgg ctctacgtca cgaagtactt tcctccgtcg gagaaagccc
     1861 gcgccgagga gatggttcgg aacgagatcg cggcgttcgc cgcccggatc gaccggctcg
     1921 actggatggc gccccagacg aaggcccggg ccaaggcgaa gctcgcggtg ctcaaggtcg
     1981 gcgtcggata tccggacaag tggaccgact actccgggct cgaggtcgtg aagggcgacg
     2041 cgctcggaaa cgcccggagg gcgtctctct tccgcacggc gcgcgccctc gcccggctgg
     2101 ggaagccggt cgaccgcggc gagtgggtca tgaacccgca gctcgtgaac gcggtgaacc
     2161 tccccgcgat gaacgccatg aacttcccgg cggcgatgct ccagccgcct tacttcgacc
     2221 cgaagcggcc cgccgtcatg gactacggcg cgtcgggctc cgtgatcgga cacgagatca
     2281 gccacagctt cgacgaccag ggcgcgctct tcgacgccga agggcggctg aagaactggt
     2341 ggacgaagga ggacttcgag cacttcaccg cgtcggccca ggcgctgatc cggcagtaca
     2401 acgcgtaccg tcccttcccg gacctcgccg tgaacggcaa gctcacggtc agcgagaaca
     2461 tcgccgacgt cgcgggcctc gcggcggcgt acgacgcgta ccggatctcg cacggcggca
     2521 aggaggcgcc cgcgtggcgc ggcgtttcgg gcgaccagca gttcttcatc gccttcgccc
     2581 agagctggcg gacgaagttc cgcgaggccg cgctgaggca gcggatcgtg atcgacagcc
     2641 acgcgccggc cgagtaccgc gcagacacgg tccgcaacct cgacgcctgg taccccgcgt
     2701 tcgacgtgaa gccggggcag aagctctacc tggcgccgaa ggatcgcgtc aaggtctggt
     2761 gaggccgggg cctcggtgcg ccgggaccct gaacccgccg tgcgccgttc cggcgcggcg
     2821 ggaggggaag gagagtggtg gagctgaacg ggatcgaacc gtcgacctcc tgagtgcgat
     2881 aaaggctgcc tccctcattt tcccagcaaa ttctgagact tagcgagcaa gggagcgcct
     2941 ttcctgggac ttctgagcgt gccgaatcag tgtacgcctg gaattcgcgg agcgggattt
     3001 tcgcgaaagg ctgtcaagcg gaaaaaggcc ttctaggact ggtccccttt ggcagccaat
     3061 cgagacgggt ttcgttttga cgaaaagccg cggcgcctga ccgtcacgcc tcggcttgga
     3121 ttgcacttcc cccgctgacg gcccgcgacg tcacgccgct tgctgcggct cgcgcgcgac
     3181 cacctgcgcg cgcgggtaca cgtgctcggt cgtcgcgctc tcgcgcaagc ggcgcgccgc
     3241 gctaccggct ctcgaccgcg gcgcgctgat ccacg
//
LOCUS       DAOMFY010000369         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_12529_length_3215_cov_8.652848, whole genome shotgun sequence.
ACCESSION   DAOMFY010000369 DAOMFY010000000
VERSION     DAOMFY010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12529_length_3215_cov_8.652848"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..782)
                     /locus_tag="PLB01_18720"
     CDS             complement(30..782)
                     /locus_tag="PLB01_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ79388.1"
                     /translation="MTAAPESLTMFFPCFNDAATIGSLVAAAHVVGTESGRAFEILVV
                     DDGSKDNSLEILEALREKYPELRVVPHGTNRGYGAALRSGFSNAANDLVFYTDGDGQY
                     DVFELRKFLPVLQEGVDVVNGYKIARSDPFHRIVIGKVYLVLMRILFQFHVRDVDCDF
                     RLIRRSALGAISLKHSSGVICLELVKKLELAGFRFVDFPVHHYHRVAGKSQFFNFRRL
                     FATGVNILRLWWEIHVVRDTPRGVAHAPAGAP"
     gene            827..1264
                     /gene="rpiB"
                     /locus_tag="PLB01_18725"
     CDS             827..1264
                     /gene="rpiB"
                     /locus_tag="PLB01_18725"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQQ79389.1"
                     /translation="MKIALGSDHAGFAYKETIKAMLLRTGHDVRDFGTSSPDMVDYPL
                     FVRPAAEAVASGACDRGIVLGGSGNGEAIVANKVKGVRCALCWSEATAKAAREHNDAN
                     VLSLGERMISEETALAVVRVFLETPFDPAAGGGRHARRIAMIE"
     gene            1302..2936
                     /locus_tag="PLB01_18730"
     CDS             1302..2936
                     /locus_tag="PLB01_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQQ79390.1"
                     /translation="MIALSGINKQYGRQVLFVDASFQLNDGEKIGLVGPNGAGKTTIF
                     RLITGEEHADDGDVSVPKKASIGYFKQEIDEMAGRPVLDEAIAGSGRLGDLHHELIDL
                     EHALADPVRAGDMEEILERFGEVQGEYQQRGGYELEARAREVLHGLGFDDERIDGDVG
                     ALSGGWKMRVAMAKVLLGRPDILLMDEPTNHLDIESILWLEGYLKEYPGSLLMTCHDR
                     DFMNRVVTKVVEIDGGETITYSGNYDFYERERLQRESQREAAFARQQAMLAKEQRFIE
                     RFQTHAAKAAQVQSRVKKLEKIETIELPKKRAVVRFDFKAPARSGDDVAILEKLSKAY
                     GTRRLYSDFDFLVRRGERWCVMGVNGAGKSTLLKMVAGMLPPDAGSVRVGASVKLGYF
                     SQQALDVLDPELTVLEQVQKDFPAAGIGVLRALVGAFQFSGDDVDKKVRFLSGGEKSR
                     LVMARMLFDPPNFLVLDEPTNHLDLATKEMLVESLSKFEGTMLFVSHDRAFLRGLSNR
                     VLVLSENDSSDPPHAYGGSYAEWVSRTGHEAPGVHA"
     gene            2944..>3215
                     /locus_tag="PLB01_18735"
     CDS             2944..>3215
                     /locus_tag="PLB01_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79391.1"
                     /translation="MSRPRFAAALAVLACVALAGAERARFSLWTTSDEPPHLAAAREW
                     RYGPGMVSNFEHPVLMKVIAGSFLPAQKPALEIDETRAGRAAIPFVL"
BASE COUNT          532 a         1109 c         1092 g          482 t
ORIGIN      
        1 ccgcgcgagg tcgagatacg cgacgccggt cacggcgcgc ccgccggcgc gtgggcgacg
       61 ccgcgcggcg tgtcgcgcac gacgtggatc tcccaccaga gtctcaggat gttcacgccg
      121 gtcgcgaaga gacggcggaa gttgaagaac tgggacttcc ccgcgacgcg gtggtagtgg
      181 tgaaccggga agtccacgaa ccggaagccc gcgagctcga gcttcttcac gagctcgagg
      241 cagatcaccc cgctcgagtg cttcagcgag atcgcgccga gcgccgaacg gcggatgagg
      301 cggaagtcgc agtccacgtc gcgcacgtgg aactggaaga ggattcgcat gagcacgagg
      361 tagaccttcc cgatcacgat ccggtggaac gggtcggagc gggcgatctt gtagccgttc
      421 acgacgtcga cgccttcctg caggaccggg aggaacttcc tgagctcgaa gacgtcgtac
      481 tggccgtcgc cgtccgtgta gaagacgagg tcgttcgccg cgttagagaa gccggagcga
      541 agggcggctc cgtagccgcg gttcgtcccg tgcgggacga cgcgcagctc ggggtacttc
      601 tccctcaggg cctcgaggat ctcgaggctg ttgtccttcg acccgtcgtc cacgacgagg
      661 atctcgaagg cccgtcccga ctccgtgccg acgacgtgcg cggccgcgac gagcgatccg
      721 atcgtcgccg cgtcgttgaa gcacgggaag aacatcgtca gactctcggg cgcggccgtc
      781 atgcggtccg ccattgtatc gggcgccacg ggtagactcg gcggccatga agatcgcgct
      841 cggctccgac cacgccggct tcgcatacaa ggagacgatc aaggcgatgc tgctccgcac
      901 ggggcacgac gtgagggact tcgggacctc ctcgcccgac atggtcgact atcccctctt
      961 cgtgcggccc gcggccgagg ccgtcgcaag cggcgcgtgc gaccgcggca tcgtcctcgg
     1021 cggctcggga aacggcgagg ccatcgtcgc gaacaaggtg aagggcgtgc gctgcgcgct
     1081 ctgctggagc gaggcgaccg cgaaggccgc ccgcgagcac aacgacgcga acgtcctctc
     1141 gctcggcgag cggatgatct ccgaggaaac cgccctcgcg gtcgtgcgcg tgtttctcga
     1201 aaccccgttc gacccggcgg ccggcggcgg ccgccacgcg cggcggatcg cgatgatcga
     1261 gtgaggcgaa acgggcgccg gcctgctatc gtccgccgcc catgatcgcg ctctccggca
     1321 tcaacaagca gtacggccgc caggtcctct tcgtcgacgc gtcgttccag ctgaacgacg
     1381 gcgagaagat cggcctcgtc gggccgaacg gcgccggcaa gaccacgatc ttccgcctca
     1441 tcaccggcga ggagcacgcg gacgacggcg acgtctccgt gccgaagaag gcctcgatcg
     1501 gctacttcaa gcaggagatc gacgagatgg ctggacggcc cgttctcgac gaggcgatcg
     1561 cggggagcgg ccgcctcggc gatctccacc acgagctgat cgacctcgag cacgcgctcg
     1621 cggacccggt gcgggcgggc gacatggagg agatcctcga gcggttcggc gaggtgcagg
     1681 gcgagtacca gcagcggggc ggctacgagc tcgaggcgcg agcccgcgag gtcctccacg
     1741 gcctgggctt cgacgacgag cgcatcgacg gcgacgtggg cgctctctcg ggcggctgga
     1801 agatgcgcgt cgcgatggcc aaagtgctcc tcggcaggcc cgacatcctc ctcatggacg
     1861 agccgacgaa ccatctcgac atcgagtcca tcctctggct ggagggatac ctcaaggagt
     1921 atcccggctc cctcctcatg acgtgccacg accgggactt catgaaccgc gtcgtcacga
     1981 aggtcgtcga gatcgacggc ggcgagacga tcacttattc cggcaactac gacttctacg
     2041 agcgcgaacg actccagcgc gagtcccagc gcgaggcggc gttcgcgcgc cagcaggcga
     2101 tgctcgccaa ggaacagcgc ttcatcgagc ggttccagac ccacgccgcg aaggccgcgc
     2161 aggtccagag ccgcgtcaag aagctcgaga agatcgagac gatcgagctc ccgaagaaac
     2221 gcgccgtcgt gcgcttcgac ttcaaggcgc ccgcgcgctc cggggacgac gtggcgatcc
     2281 tcgaaaagct ctcgaaggcg tacggcacgc ggcgcctcta ttcggacttc gacttcctgg
     2341 tccggcgcgg cgagcgctgg tgcgtcatgg gcgtgaacgg cgccgggaag tccacgctcc
     2401 tgaagatggt cgcgggcatg ctcccgccgg acgcgggctc cgtgcgcgtc ggcgcgagcg
     2461 tgaaactcgg ctatttctcg cagcaggcgc tcgacgtcct cgaccccgag ctgacggtgc
     2521 tcgagcaggt ccagaaggac ttccccgccg ccggcatcgg cgtcctccgc gccctcgtcg
     2581 gcgcgttcca gttctccggg gacgacgtcg acaagaaggt ccgcttcctc tcgggcggcg
     2641 agaagtcgcg cctcgtcatg gcgcgcatgc tgttcgaccc gccgaatttc ctcgtcctcg
     2701 acgagcccac gaaccacctc gacctcgcga cgaaggagat gctcgtcgaa tcgctctcga
     2761 agttcgaagg gacgatgctc ttcgtctcgc acgaccgcgc gttcctccgc gggctctcga
     2821 accgcgtcct cgtgctctcc gagaacgact cgtccgaccc gccccacgcc tacggcggct
     2881 cgtacgccga gtgggtgtcg cgcacggggc acgaggcgcc cggcgtccac gcataatcgg
     2941 ccgatgtccc gcccgcggtt cgcggccgcc ctggcggtcc tcgcctgcgt cgccctcgcg
     3001 ggcgccgagc gggcgcgctt ttccctctgg acgacgtccg acgagccgcc gcacctcgcg
     3061 gcggcccgcg agtggcggta cggaccgggg atggtctcca acttcgagca tcccgtcctc
     3121 atgaaggtga tcgcgggctc gttcctgccc gcgcagaaac ccgctctcga gatcgacgag
     3181 acccgtgcgg gccgcgcggc gatcccgttc gtcct
//
LOCUS       DAOMFY010000370         3208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_12588_length_3208_cov_6.696480, whole genome shotgun sequence.
ACCESSION   DAOMFY010000370 DAOMFY010000000
VERSION     DAOMFY010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12588_length_3208_cov_6.696480"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLB01_18740"
     CDS             complement(<1..564)
                     /locus_tag="PLB01_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ79392.1"
                     /translation="MKPTLIGMRDIEKTYSVGEEKVRALRGVTFTIDRGEYVAIMGPS
                     GSGKSTLMNLIGCLDTPTGGEYLLNGTPVQTLTDDELARIRNREIGFVFQTFNLLART
                     SALAQVELPLVYSGLPKKERRERAEKALERVGLTDRAHHNPNELSGGQRQRVAIARAL
                     VTGPSLLLADEPTGNLDSATGEDIMRLF"
     gene            complement(561..1304)
                     /locus_tag="PLB01_18745"
     CDS             complement(561..1304)
                     /locus_tag="PLB01_18745"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HQQ79393.1"
                     /translation="MSEPAPAAPVPGIGPVEAVVGTFTKPSDTFGRLVARPTWWLPLV
                     LTLLTTAGSFWIVMPKIDMDRTIRESIEKRMEKSGRTASPEVVQRQVEAAKKMTPLYL
                     GIGVVAATVFFFVIALVLWGGAKAMGADARYAQLLAIWGHSGLPSLVGVLISIPIFLQ
                     LPDASLTQEGAQHVLKSNLGAFLDESTPAALHTLASSVDVFSFAVLFLLVLGFRRLPG
                     LSKGAATWTPIVLWGLAVAVKVAWKAVMR"
     gene            complement(1301..2965)
                     /locus_tag="PLB01_18750"
     CDS             complement(1301..2965)
                     /locus_tag="PLB01_18750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQQ79394.1"
                     /translation="MSPEQKPGSPVRAGRRAAAAALALVLSAGVAFADDSVSVPVASR
                     ARKSLPIPQGDKIELTLQKTIEMTLQNVLDLDVAAYNLEESHFGILSARGAFDPNFEL
                     DLGIKNTETPRSSIYQANQTKTGYANPYLTGLLPYGTTYTLGWTNTRSDVVGAGTLLN
                     PTYSSNLSLGVTQPLLRNFGKTVTERFVVQAKLGENQSGYGFVIAVQTAIQTAENAYW
                     DLVYAVENLKAKQEALDRAKDLNRITKIKIDVGALAPIDIVQTEVTIAQREQDIIIAE
                     GLIGDAQDRLRRLLNVQSVPDWNRPIVPTDRPTRESLRTGFVADVNQGYESALKVRPE
                     IRQALLNIESKKVTAAYQQNQLKPRLDLSGGYGRAGLGANGLGVDADGNPYDLSYSSA
                     LTQIGRGDYPAWNVGLVFAIPIGNRAAKGNAAIANADLELARTSLAITKANLHVEVRA
                     AARNIDTAYRSVQAASKARELAERNLDAEKKKYENGMTTSFQVAQIQNDLTTARTIEL
                     QAVAGYLKSLVSWHKAIGDLLPQKNVVLSGLNVSLDPTPAEEGAVR"
BASE COUNT          490 a         1025 c         1156 g          537 t
ORIGIN      
        1 gaagagccgc atgatgtcct cgcccgtcgc cgagtcgagg ttgccggtcg gctcgtcggc
       61 gagcaggagg gacggccccg tgacgagcgc gcgcgcgatc gcgacgcgct ggcgctggcc
      121 gcccgagagc tcgttcgggt tgtggtgggc gcggtccgtc aggcccacgc gctcgagagc
      181 cttctcggcc cgctcacggc gctccttctt cggaagcccc gagtagacga gcggcagctc
      241 gacctgggcc agagcgctcg tccgcgcgag gaggttgaac gtctggaaga cgaagccgat
      301 ctcgcgattc cggatgcgcg cgagctcgtc gtccgtcagc gtctggacgg gcgtcccgtt
      361 cagcaggtac tcgccgccgg tcggcgtgtc gaggcagccg atgaggttca tgagcgtcga
      421 cttgccggag ccggaggggc ccatgatcgc gacgtactct ccgcggtcga tcgtgaacgt
      481 cacgccgcgc agggcgcgga ccttctcctc gccgacggaa tacgtctttt cgatgtcgcg
      541 catcccgatc agcgtcggct tcatctcatg accgccttcc aggcgacctt gacggcgacc
      601 gcgaggcccc acaggacgat gggcgtccac gtcgcggcgc ccttggagag ccccggcagc
      661 ctgcggaacc cgagaacgag gaggaagaga accgcgaacg agaagacgtc caccgacgac
      721 gcgagggtgt gcagcgcagc cggtgtcgac tcgtcgagga acgcgccgag attggacttc
      781 agcacgtgct gcgcaccctc ctgcgtgaga gaggcgtccg gcagctggag gaagatcggg
      841 atcgagatca ggacgccgac gaggcttgga agccccgagt gaccccagat cgcgaggagc
      901 tgcgcgtacc gggcgtccgc tcccatcgcc ttcgcaccgc cccagaggac gagcgcgatc
      961 acgaagaaga agacggtcgc ggcgacgacg ccgatgccga ggtagagcgg cgtcatcttc
     1021 ttcgccgcct cgacctgacg ctggacgacc tcgggcgaag ccgtgcggcc gctcttttcc
     1081 atgcgcttct cgatcgactc gcggatcgtg cggtccatgt cgatcttcgg catcacgatc
     1141 cagaaggacc cggcggtcgt gagcagcgtg aggacgagcg ggagccacca cgtcggacgc
     1201 gcgacgagcc gcccgaacgt gtcggacggc ttcgtgaacg tgccgacgac ggcctcgacg
     1261 ggaccgatgc ccgggacggg ggccgcgggt gcgggctcgc tcaccggacg gctccctctt
     1321 ccgccggcgt cgggtcgagc gacacgttca ggccgctcag gacgacgttc ttctgcggga
     1381 gcagatcgcc gatcgccttg tgccaggaga cgagggactt gaggtagccc gcgaccgcct
     1441 gaagctcgat cgtgcgcgcg gtcgtgaggt cgttctggat ctgcgcgacc tggaacgacg
     1501 tcgtcatccc gttctcgtac tttttcttct cggcgtcgag gttgcgctcc gcgagctcgc
     1561 gcgccttcga ggcggcctgc acgctgcggt acgccgtgtc gatgttacgt gcggccgcgc
     1621 gaacctcgac atgcaggttc gctttcgtga tcgcgaggct ggtccgtgcg agctcgaggt
     1681 cggcgttcgc gatggcggca ttgcccttcg cggcgcggtt cccgatcggg atcgcaaaga
     1741 cgaggccgac gttccacgcg ggatagtcgc cgcggccgat ctgggtcagc gccgaggagt
     1801 aggagagatc gtacgggttt ccgtcggcgt cgacgcccag cccgttcgcc ccgagacccg
     1861 cccggccgta gccgccggag aggtcgaggc gcggcttgag ctggttctgc tggtacgcgg
     1921 ccgtgacttt cttcgactcg atgttcagga gggcctgccg gatctcgggc cggaccttca
     1981 gcgccgactc gtagccctgg ttcacgtccg cgacgaagcc ggtgcggagc gactcgcggg
     2041 tggggcgatc cgtcgggacg atcgggcgat tccagtcggg aaccgactgg acgttcagga
     2101 gccgccggag ccggtcctgg gcgtcgccga tgaggccttc ggcgatgatg atgtcctgct
     2161 cgcgctgggc gatcgtgacc tcggtctgga cgatgtcgat cggcgcgagt gcacccacgt
     2221 cgatcttgat cttcgtgatc cggttcaggt ccttggcccg gtcgagagcc tcctgcttcg
     2281 ccttgagatt ctcgaccgcg tagacgaggt cccagtaagc gttctcggcg gtctggatgg
     2341 ccgtctgcac cgcgatgacg aagccgtagc cgctctggtt ctctccgagc ttcgcctgga
     2401 cgacgaagcg ctccgtcacc gtcttgccga agttccggag gaggggctgc gtcacgccga
     2461 gcgagaggtt gctggaatag gtcgggttca ggagcgtccc cgcgccgacg acgtcgctgc
     2521 gcgtgttcgt ccagcccagg gtgtacgtcg ttccgtacgg cagcagcccc gtcaggtaag
     2581 ggttcgcgta gccggtcttc gtctggttgg cctggtagat cgagctccgg ggcgtctccg
     2641 tgttcttgat cccgaggtcc agctcgaagt tcgggtcgaa ggcgccgcgc gccgagagga
     2701 tgccgaagtg cgactcctcg aggttgtacg ccgcgacgtc caggtcgagg acgttctgga
     2761 gcgtcatctc gatcgtcttc tggagggtca gctcgatctt gtcgccctgg gggatcggaa
     2821 gggacttgcg ggcccgcgac gcaacgggca ccgagacgga gtcgtccgcg aaggcgacac
     2881 cggcggaaag gacgagggcg agggccgccg cggccgcccg ccggccggcg cgcacgggac
     2941 tgccgggctt ctgctcagga ctcacgaaaa cctcctcgaa ccgagagccg atgatagtgg
     3001 acgggcaccg tctgaaagta cggcatccgg gcggaaaggt ttccgtgaga aagggtttct
     3061 tcagttttcc aggggggcgt ggcaggccgc agcgcgctcg ggattgcccg gatccacgag
     3121 caggcgcggg acctcctgcg cacagaccgg ccgcgcggac gggcaccggg gatggaaggg
     3181 gcaacccgga ggcggcgcgg ctggcgaa
//
LOCUS       DAOMFY010000371         3193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_12679_length_3193_cov_7.043977, whole genome shotgun sequence.
ACCESSION   DAOMFY010000371 DAOMFY010000000
VERSION     DAOMFY010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12679_length_3193_cov_7.043977"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PLB01_18755"
     CDS             complement(<1..1225)
                     /locus_tag="PLB01_18755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQQ79395.1"
                     /translation="MESPVIPAAGAQEALGFGPLETVFEALGRALIVLDADFRIIRAS
                     HTLDEISGMGIVESAIGKPIEELVGAKLFGPADTLREALTSGRREEGRRAVLRCGAKS
                     ARLVSLTAAVVPLHVSNHCDPRARYLVVVRPAEDEDSLLQSMIASHGLVARSPAMLKI
                     VHLVESLHRSNATVLITGESGTGKEVIARALHSSSLQSSGPFVAVNCGALPGDLLESE
                     LFGHVKGAFTGAVRDRVGRFELARGGTIFLDEVGDIPLHLQVKLLRVLQERQFERVGE
                     SRTRPMEARIIAATNQDLHGAVQTGRFRDDLYYRLRVVPIHIPPLRERPEDVALIAQH
                     QLAHIGGRAGRALLLSPDTLEALKAYPWPGNVRELENALEYAVALCTGQTIQIEDLPE
                     EIRSGGAAAGPPRPAA"
     gene            1395..>3193
                     /locus_tag="PLB01_18760"
     CDS             1395..>3193
                     /locus_tag="PLB01_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79396.1"
                     /translation="MTKKYSLLLLGAAAGLLFALRPGGAAGPTGWTVVAWNNLGMHCM
                     DADFGVFAILPPYNTIQAQVVDASGKRVTDPARAVLTYEAVADPSGSINRTSAGKTNF
                     WASVLSLFGASPAVDTGLLGKKMPGAANAPQPMGWDPALQWWIAEGIPITPYDDAGRK
                     NFYPLMKVTAKSTTGAVLASTNIVLPVSDEMDCSACHSSSSGPAARPYGGWIHDANPQ
                     RDYRLNILRLHDDMNASNASYTAALAALGFNPAGLSATVAGGKSILCASCHGSEALAG
                     SGRAGIPPLTQAMHARHARVLDPTNGLALDAVSNRSACYRCHPGSETKCLRGAMGAAV
                     AADGSMLMQCQSCHGGMSAVGAAGRTGWLQEPSCQECHAGTATVNDGQIRYTSVFDVH
                     GNPRAPASTVFATNADTPAAGLNLYRFSRGHGGLQCSACHGSTHAEYPSSHANDNLQS
                     IALQGHAGTLSECSTCHNASVPSTVNGGPHGLHPLGQSWVSGHESAAKSNLAACRACH
                     GTDDRGTVLSRAFTSRTFSTEHGTVKLAAGALVGCYTCHNGPSGEGNPPTPTPTPRQT
                     PSATPGSTPGSSPTPTRVPPTATPTPPPPTATPT"
BASE COUNT          473 a         1187 c         1091 g          442 t
ORIGIN      
        1 gagccgccgg gcgcggcggt ccggccgcgg ccccgccgga gcggatctct tcgggcaggt
       61 cctcgatctg gatcgtctgg cccgtgcaga gggccaccgc gtactcgagc gcgttctcga
      121 gctcccgcac gttgcccggc cacgggtacg ccttgagcgc ctcgagcgtg tcgggcgaga
      181 ggaggagcgc gcggcccgcg cgtccgccga tgtgggcgag ctggtgctgg gcgatgagcg
      241 cgacgtcctc gggccgctcg cggagcggcg ggatgtggat cggcacgacg cgcagccggt
      301 aatagaggtc gtcgcggaat cggcccgtct ggacggcgcc gtggaggtcc tggttcgtcg
      361 ccgcgatgat ccgcgcctcc atcggacgcg tccggctctc cccgacccgc tcgaactgcc
      421 gctcctgcag gacgcgcagg agcttgacct gcaggtggag cgggatgtcg ccaacctcgt
      481 cgaggaagat cgtcccgccg cgcgcgagtt cgaagcgtcc gacgcggtcg cggaccgcgc
      541 ccgtgaacgc acccttcacg tgcccgaaga gctcgctctc gaggaggtct ccgggaaggg
      601 ccccgcagtt cacggcgacg aacggccccg agctctggag cgagctcgag tggagcgcgc
      661 gcgcgatgac ttccttgccc gtcccgctct cccccgtgat gaggaccgtc gcgttgctcc
      721 ggtggagcga ctcgacgagg tggacgatct tgagcatcgc gggcgaccgc gcgacgaggc
      781 cgtgcgacgc gatcatgctc tggagaagcg agtcctcgtc ctcggccggg cggacgacga
      841 cgaggtagcg cgcccgcggg tcgcagtggt tcgagacgtg aagcgggacc acggccgcag
      901 tgagcgagac gaggcgggcg ctcttggcgc cgcagcggag aacggcgcgc cgtccctcct
      961 cgcgccggcc gctcgtgagc gcctctcgga gcgtgtccgc ggggccgaag agcttcgcgc
     1021 cgacgagctc ctcgatgggc tttccgatcg cggactcgac gattcccatc cccgagatct
     1081 cgtcgagcgt gtggctggcg cggatgatcc ggaagtcggc gtcgaggacg atgagcgccc
     1141 ggcccagcgc ctcgaagacg gtctcgagcg gcccgaagcc gagggcttcc tgcgcaccag
     1201 ccgcggggat cacggggctt tccaccggga gaattatccg cccgcgtggc gttcggattc
     1261 gccctctagt acagctgcat cagagagacg gcgtcgcaac gttaggcaga cgggggggtc
     1321 tgcactcctc cacgagtccg ccgggctttt cacgatcggg tgtaccggcc gagccgcatc
     1381 gaaaaggaga ctatatgact aaaaagtatt cattactcct cctcggcgcg gcggccggac
     1441 tcctcttcgc gctccgtccc ggcggcgccg cgggccccac gggctggacg gtcgtggcct
     1501 ggaacaacct cggaatgcac tgcatggacg ccgacttcgg ggtcttcgcg atcctcccgc
     1561 cctacaacac gatccaggcg caggtcgtcg acgcctcggg caagcgcgtg acggaccccg
     1621 cccgggccgt cctcacgtac gaggcggtcg ccgacccgtc cgggtccatc aaccggactt
     1681 cggccgggaa gacgaacttc tgggccagcg tcctgagcct cttcggggcc tcccccgccg
     1741 tagacaccgg tctcctcggc aagaagatgc cgggggcggc gaacgccccg cagcccatgg
     1801 gctgggatcc ggcgctccag tggtggatcg ccgaggggat tccgatcacg ccgtacgacg
     1861 acgcgggccg gaaaaacttc tacccgctca tgaaggtcac ggccaagagc acgacgggcg
     1921 ccgttcttgc gtcgacgaac atcgtcctcc cggtctcgga cgagatggac tgcagcgcct
     1981 gccactcctc gtcctcgggc cccgccgcgc ggccttacgg cggctggatc cacgacgcca
     2041 acccccagcg cgattaccgc ctcaacatcc tgcggctgca cgacgacatg aacgcgtcga
     2101 acgcgtcgta cacggcggcc ctcgcggccc tcggcttcaa tccggccggc ctctcggcga
     2161 ccgtcgccgg cggcaagtcg atcctgtgcg cctcctgtca cggctccgag gcgctcgccg
     2221 gcagcggacg ggccgggatc ccgccgctca cgcaggcgat gcacgcgcgg cacgcgcgcg
     2281 tcctcgaccc gacgaacggc cttgcgctcg acgcggtctc gaaccgctcg gcgtgctatc
     2341 gctgccaccc gggctccgag acgaagtgcc tgcgcggcgc catgggcgcc gccgtcgcgg
     2401 cggacggttc gatgctcatg cagtgccaga gctgccacgg cgggatgagc gccgtcggcg
     2461 ccgccggccg cacggggtgg ctgcaggaac cgtcctgcca ggagtgccac gccgggaccg
     2521 caaccgtgaa cgacggccag atccgctaca cgtcggtctt cgacgtgcac gggaacccgc
     2581 gcgctccggc gagcaccgtc ttcgcgacga acgccgacac gcccgccgct gggctcaacc
     2641 tctaccggtt ctccagggga cacggcgggc tgcagtgctc cgcctgccac ggctcgacgc
     2701 acgccgagta cccgtcctcc cacgcgaacg acaacctcca gagcatcgcg ctccagggcc
     2761 acgcgggaac gctctctgag tgctcgacgt gccacaacgc gtccgtcccc tcgaccgtca
     2821 acggagggcc gcacggcctc cacccgctcg ggcagtcctg ggtgagcggc cacgagagcg
     2881 ccgcgaaatc gaacctcgcc gcctgccgcg cgtgccacgg cacggacgac cgcgggacgg
     2941 ttctctcgcg cgccttcacg agccggacgt tcagcacgga gcacggtacc gtgaagctcg
     3001 cggccggcgc gctcgtcggt tgctacacgt gtcacaacgg cccttcgggc gagggcaacc
     3061 cgccgacgcc cacgcccaca ccgcgccaga cgccgtccgc gacgcccggc tccacgccgg
     3121 gctcctcgcc cacgccgacc cgcgtgcctc cgaccgcgac gccgacgccg ccccctccga
     3181 cggcgacgcc cac
//
LOCUS       DAOMFY010000372         3168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_12831_length_3168_cov_7.006746, whole genome shotgun sequence.
ACCESSION   DAOMFY010000372 DAOMFY010000000
VERSION     DAOMFY010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12831_length_3168_cov_7.006746"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLB01_18765"
     CDS             complement(<1..609)
                     /locus_tag="PLB01_18765"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HQQ79397.1"
                     /translation="MTSRQRVIGMGLLWLVCVWAVALVSPHVVPWMTGTNPWTWDVVR
                     RVTPLTRWDSGWYVNLAEAGYWEPPTRVGQETNHAFFPLYPGLMRLLVRTTGIETSLA
                     GNVVSAAAFLAALLLFAEWTKRHYGEERVLPAVLVLLAFPTSFFFASVYTEALVLALA
                     LAAVLSFERGRPIAGAAAGVLAGLTRITGLVLAPYLALVAWRS"
     gene            640..1419
                     /locus_tag="PLB01_18770"
     CDS             640..1419
                     /locus_tag="PLB01_18770"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ79398.1"
                     /translation="MNDREYEVMFRREDAYWWYRGMREVTRAIAPGLYACPRSARVLD
                     AGCGTGRNLVDLAAAGPAVGVDVSLRALGFARRRGAAPLVCASVEALPFRAGAFEAVL
                     SRDVLYMVPDDARAAREIARVLAPGGTLALSAPAFDALAGAHDVAVGALRRYTTGGLE
                     RLLKGAGLAVVRTSYANFFLAGPIGFLRKVTGAKARGVPRKDVRSDFGLAPRPFEGIF
                     FFLLSLEARILGMKGRLPFGVSALVLAAKPGQGAGTSEAGK"
     gene            complement(1386..>3168)
                     /locus_tag="PLB01_18775"
     CDS             complement(1386..>3168)
                     /locus_tag="PLB01_18775"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQQ79399.1"
                     /translation="STPVAGGCEGTPIPDDAPGGLVCDTIVSSFVGNVSTLFLALEID
                     HPDPTQLVVTLTGPDGTAATVLAHGGHPGEAVREIFGLTKGSFEPLSVFAGNTLEGTW
                     RLTVTDSVAGGAGRLVRWALFVEPSIPEPEPDFPGATSFVATSAHRIGKLGAFYTTDV
                     RLFNTDPFRTQDVRLRFSPAGGAPRTLTVTLPPLSTRALGDVVHDTFRMDGYGPLFLA
                     APPAVVAASRTSTTAPRGGSFGLSVPADAAASAAGAGATLVLVPCFKATGFRVNVGVT
                     EVSGQTAAVEIAVRDSSGTLRAVIPRAVPAAGLVQVNDIYALTRLAPDAADRIEVRVT
                     AGPGRVAAWATPVDDSSNDGSFVSAHPASASLLIPAAARSAGQFGARFVTDLKVSNAG
                     LDPVTVRVAFSPLSGPAPDPAFVVLRGSETRAFDDVLGTLFGATADTAGTLRLSASGG
                     GFLHASSRTSTTDHGRSYGLAIDPAAGAALAGPGRQLALTFLSSSAARRTNVGFVETA
                     GLATRVSATLLAPNGVRVATRDFGLDPLGAVQWNDVFAEMEAPPLADASLVVDVVSGG
                     SVAAYAILVDNRTNDASYFPASLVPAP"
BASE COUNT          492 a         1057 c         1215 g          404 t
ORIGIN      
        1 cgagcgccac gcgacgagcg cgaggtacgg tgcgaggacg aggcccgtga tccgcgtcag
       61 ccctgcgagg acgccggcgg cggctccggc gatcggccgg ccgcgctcga acgacaggac
      121 ggccgcgagg gcgagcgcga ggacgagcgc ctcggtgtac acggaggcaa agaagaacga
      181 cgtcgggaac gcgagcagga cgagcaccgc gggcaggacg cgctcctcgc cgtaatgccg
      241 cttcgtccac tccgcgaaga ggagcaacgc cgcgaggaac gcggcggcgg agacgacgtt
      301 ccccgcgagg gacgtctcga tccccgtcgt ccggacgagg aggcgcatga ggccggggta
      361 gagggggaag aacgcgtggt tcgtctcctg tccgacgcgg gtcgggggct cccagtagcc
      421 ggcttcggcg aggttcacgt accagcccga atcccagcgg gtgagcggcg tgacgcggcg
      481 gacgacgtcc caggtccacg ggttggtccc ggtcatccac ggcacgacat ggggcgaaac
      541 gagcgcgacg gcccacacgc agacgagcca caggaggccc atcccgatca cacgctggcg
      601 cgaggtcacg accgggcgga ttataggcag cgagtgggga tgaacgaccg cgaatacgag
      661 gtgatgttcc gccgcgagga tgcctactgg tggtaccgcg gcatgcgcga ggtcacgcgt
      721 gcgatcgcgc ccggcttgta cgcgtgtccg cggtccgccc gcgtcctcga cgccggttgc
      781 gggacggggc gcaacctcgt ggacctcgcc gcggccggtc cggcagtcgg cgtggacgtg
      841 tcgctgcgcg ccctcggttt cgcgaggcgc cgcggggccg cgccgctcgt ctgcgcgtcc
      901 gtcgaggctc tgccgttccg cgcgggggcc ttcgaggcgg tcctgtcccg ggacgtcctt
      961 tacatggttc cggacgacgc ccgcgccgcg cgcgagatcg cccgtgttct cgcgccgggc
     1021 gggacgctcg cgctctcggc cccggctttc gacgcgctcg cgggcgcgca cgacgtcgcg
     1081 gtcggcgccc tccggcgcta cacgacggga ggccttgaaa ggctgctgaa aggcgctggg
     1141 ctcgcggtgg tccgcacgag ctacgccaat ttcttcctgg cggggcccat cggatttctc
     1201 agaaaggtaa caggagcgaa ggcccgcggc gttcctcgca aggacgttcg aagcgatttt
     1261 ggcctcgcgc ccagaccatt cgaaggaatc ttcttcttct tgctttcgct cgaggcgcgc
     1321 atcctgggga tgaaaggacg gctgcccttt ggagtctccg cgctcgtcct cgcggcgaaa
     1381 cccggtcagg gcgccggaac gagcgaagcg gggaagtagc tcgcgtcgtt cgtccggttg
     1441 tcgacgagga tcgcgtacgc ggcgacgctc ccgcccgaga cgacgtcgac gacgagcgac
     1501 gcgtcggcca gcggcggggc ctccatttcg gcgaagacgt cgttccattg aaccgctccg
     1561 aggggatcga gaccgaagtc ccgagtcgcg acgcggactc cgttcggggc gagcagcgtc
     1621 gcggagacgc gcgtcgcgag tccggccgtt tcgacgaagc cgacgttcgt ccggcgggct
     1681 gcagaggacg acaggaacgt gagcgcgagc tggcggccgg gccccgccag cgcggcaccg
     1741 gctgcagggt cgatcgcgag gccgtaggag cggccgtgat ccgtcgtcga cgtgcgcgag
     1801 gacgcatgca ggaagccgcc tccggacgcg gagaggcgca gtgttcccgc ggtgtcggcg
     1861 gtggcgccga acagcgtgcc gaggacgtcg tcgaacgcgc gcgtctcgct cccgcggaga
     1921 acgacgaagg cgggatcggg cgccggcccg gacagagggg agaacgcgac ccggacggtc
     1981 acgggatcga gtcctgcgtt cgagaccttc aggtcggtca cgaaacgcgc accgaactgc
     2041 ccggccgaac gcgccgccgc cggaatgaga aggctcgcgg acgccgggtg cgcggagacg
     2101 aaggagccgt cgttcgagga gtcgtccacg ggcgtcgccc acgcggcgac gcggcccggg
     2161 ccggcggtga cgcgcacttc gatccggtcg gcggcgtccg gcgcgaggcg cgtgagcgcg
     2221 tagatgtcgt tgacctgcac gaggcccgcc gccggcacgg cgcgcggaat cacggcgcgc
     2281 agagtgccgg acgagtcgcg cacggcgatc tcgacggcgg ccgtctggcc ggagacctcc
     2341 gtcaccccga cgttcacgcg gaagccggtg gccttgaagc acggcacgag gacgagcgtc
     2401 gcgcccgccc ccgcagccga ggcggcggcg tcggcgggga ccgagagccc gaacgagccg
     2461 ccgcgcggcg cggtcgtgga cgtgcgggat gcggcgacca cggccggagg ggcggccagg
     2521 aagagcggcc cgtagccgtc catccggaac gtgtcgtgca cgacgtcccc gagggcgcgg
     2581 gtcgagagcg gcggcagcgt cacggtgagg gtgcgcggag cgcccccggc aggggagaac
     2641 cgcaggcgga cgtcttgcgt cctgaagggg tcggtgttga acagcctcac atcggtcgtg
     2701 tagaacgcgc cgagttttcc gatccggtgc gcggacgtcg cgacgaagga ggtcgcgccg
     2761 ggaaaatcag gctcgggctc cggaatcgac ggctcgacga acagcgccca tctgacgagc
     2821 ctgcccgcgc ctcccgccac cgagtcggtg accgtgagcc gccacgtccc ctcgagcgtg
     2881 ttgcccgcga agacggagag cggttcgaac gagcctttgg tgaggccgaa aatttcgcga
     2941 acggcctcgc cggggtgccc cccgtgggcc aggacggtcg cggccgttcc gtccggcccc
     3001 gtgagcgtga cgacgagctg cgtcggatcg ggatggtcga tctcgagcgc gagaaagagc
     3061 gtggacacgt tgccgacgaa ggacgagacg atcgtgtcgc acacgagccc cccgggcgcg
     3121 tcgtccggga tcggcgtccc ctcgcagccg cccgcgacgg gcgtcgag
//
LOCUS       DAOMFY010000373         3153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_12936_length_3153_cov_7.277921, whole genome shotgun sequence.
ACCESSION   DAOMFY010000373 DAOMFY010000000
VERSION     DAOMFY010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12936_length_3153_cov_7.277921"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PLB01_18780"
     CDS             complement(<1..1102)
                     /locus_tag="PLB01_18780"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HQQ79400.1"
                     /translation="MATSERPTLALIDASNYLYRAFYAIRGLTGPGGRPTNAVYGFLN
                     MWRKYVADRKPAAVAVCFDRYEKTFREEMDAAYKAQRAAMPDDLVPQIEDAKRLCRLL
                     GLAVLEEAGFEADDLIGSIAVVAKEAGFRAEICSADKDLFQLVKDPDVAVFHPVKDVL
                     LDEAGVLEFFGVKPAQVIDVLALMGDSSDNIPGIRGIGEKTAKELIGTFGSLDAIYAR
                     LDEVKGKKKDLLAAGRDEAYRSQTLATVRCDRPLPAPPDKLLEHFRIRPVEGQAAKDL
                     AAFYDEMGFGRFKKELLESTSATKGAATNGGAATAAASLEPATLFLFEESSSSSSNLP
                     QESLRWVADAAGLDAVVAAAVKAGRASVHVECG"
     gene            1257..2261
                     /locus_tag="PLB01_18785"
     CDS             1257..2261
                     /locus_tag="PLB01_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQQ79401.1"
                     /translation="MSRRAAEYRAAAKTLRQLGTDLFGRRVAVASLPGVSPRAADAIA
                     SFLACGSDENVGDALAAMLDRADPAAHRKADFLSRADVDRILSAPGGISHADLRGDAH
                     LHTDRSDGRMPLAALHRALEARGDAYALLTDHARDCAVAGGLFPADFRAQRREVDALN
                     ARAGSFAMFVGAEANIAEDGSLDVTPKSVPELDAVVASVHTDLRSGRDQTARLVRAVE
                     TPGVAVLGHPKGRLYDMRSGVRADWPRVFAAAAARGVAIELNGCPERLDLPPALARLA
                     VSAGCLFSISSDAHAAAHLSFLDYGLAVARLAGVPAGHVVNTWSRDRFADWLEERGQS
                     "
     gene            complement(2252..>3153)
                     /locus_tag="PLB01_18790"
     CDS             complement(2252..>3153)
                     /locus_tag="PLB01_18790"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HQQ79402.1"
                     /translation="AGASAHPDAEIAPPPPAPPRFWDARTTTLTAGVVVGAPLLAYFA
                     WWKDSSRSTFVVAHEGWFGEDTYAGGADKASHIFWGYLGSQVLQGAYRSLGKTPGQAR
                     ALSLAVVTVTGALIELGDGYSKYGFAWEDIAANSIGAAVAYGIDAWKLDDTVGLRFGL
                     MSTPIPEACCRYGGYGDDYSKEIYTLDLKLAGLLPRMGTKSGLGRFLLLSGTYQSKGY
                     RYSPPENRRREIGIEIGLNLREILAAVGVPEDKWWGKILLGVAKYFRVPYTAIGWRYD
                     MNSGTWRGPDSGQGYDTGYIIYD"
BASE COUNT          422 a         1159 c         1104 g          468 t
ORIGIN      
        1 ggccgcactc gacgtggacg gacgcgcggc cggccttcac ggccgccgcg acgacggcgt
       61 cgaggcccgc ggcgtccgcg acccagcgca ggctctcctg agggagattc gaagaggaag
      121 acgaagactc ttcgaagaga aagagagtcg cgggctcgag ggacgcggcc gccgtcgcgg
      181 cgccgccgtt tgtcgcggcg cccttcgtcg ccgatgtgga ttcgaggagc tccttcttga
      241 agcgcccgaa ccccatctcg tcgtagaagg ccgcgaggtc cttcgcggcc tgcccttcga
      301 ccggccggat ccggaagtgc tcgaggagct tgtccggcgg cgccgggagc gggcggtcgc
      361 agcgcaccgt cgcgagcgtc tgcgagcgat aggcctcgtc gcgccccgcg gcgaggaggt
      421 ccttcttctt gcccttcacc tcgtccaggc gcgcgtagat cgcgtcgagc gagccgaacg
      481 ttccgatgag ctccttggct gtcttctcgc cgattccccg gatgccgggg atgttgtcgg
      541 acgagtcgcc catgagggcg aggacgtcga tgacctgcgc gggcttgacg ccgaagaact
      601 cgaggacgcc cgcctcgtcg aggaggacgt ccttcacggg gtggaagacc gcgacgtcgg
      661 gatccttcac gagctggaag aggtccttgt cggccgagca gatctcggcg cggaagccgg
      721 cctcctttgc gacgaccgcg atcgagccga tcaggtcgtc ggcctcgaaa cccgcctcct
      781 cgagaaccgc gaggccgagc aggcggcaga gccgcttggc gtcctcgatc tgcgggacga
      841 ggtcgtccgg catcgcggcg cgctgcgcct tgtaggcggc gtccatctcc tcgcggaacg
      901 tcttctcgta ccggtcgaag cagacggcga cggcggccgg tttgcggtcc gcgacgtact
      961 tccgccacat gttcaggaag ccgtagaccg cgttcgtggg ccggccgccg gggcccgtga
     1021 ggccgcggat cgcgtagaac gcgcggtaga ggtagttcga cgcgtcgatc agcgcgagcg
     1081 tgggccgttc ggaggtcgcc atcggcgcga gtctatccgc gacctacgta caatcgggcg
     1141 gaggcgcgat gtcggctctc ccgtctccct ccggttcttc ccttcccgcg gcgcctccgg
     1201 tgacgaacgg gcggattgcg tccgccctcc tcgcgctcgc ctccgctgaa gaccccttga
     1261 gccgccgcgc cgccgagtac cgcgcggccg cgaagaccct ccgccagctc ggcacggacc
     1321 tcttcgggcg ccgcgtcgcg gtcgcgtcgc tcccgggcgt gtcgccccgc gccgccgacg
     1381 cgatcgcgtc gtttctcgcg tgcggctcgg acgagaacgt cggcgacgcg ctcgccgcga
     1441 tgctcgaccg cgccgatccc gcagctcacc ggaaggcgga cttcctgtcg cgggcggacg
     1501 tggaccggat cctctcggcg cccggcggga tctcgcacgc ggacctgcgc ggcgacgccc
     1561 acctccacac ggaccgctcg gacgggcgca tgcccctcgc cgcgctccac cgcgccctcg
     1621 aagcgcgcgg ggacgcctac gccctcctga cggaccacgc gcgcgactgc gccgtggcgg
     1681 gcgggctctt ccccgccgac ttccgcgcgc agcggcgcga ggtcgacgcc ttgaacgccc
     1741 gcgccggctc gttcgcgatg ttcgtcggcg ccgaggcgaa catcgccgag gacggatcgc
     1801 tcgacgtcac gccgaagagc gtccccgagc tcgacgccgt cgtcgcgtcc gtccacacgg
     1861 acctccggag cgggcgcgac cagacggcgc gcctcgtgcg cgcggtcgag acgccgggcg
     1921 tcgccgtcct cggccacccg aagggccggc tctacgacat gcggagcggc gtgcgcgccg
     1981 actggccgcg cgtcttcgcc gccgccgcgg cccgcggagt ggccatcgag ctgaacggct
     2041 gcccggagcg gctcgacctc ccgcccgcac tcgcgaggct cgcggtctcc gccggctgcc
     2101 tcttctcgat ctcgagcgac gcgcacgccg ccgcgcatct gtcgtttctc gactacggcc
     2161 tcgccgtcgc gcgtcttgcg ggcgtccccg ccggccacgt cgtgaacacg tggtcgcgcg
     2221 accggttcgc ggactggctc gaggagcgcg gtcagtcgta gatgatgtag cccgtgtcgt
     2281 agccttggcc cgagtccggc cccctccacg tcccgctgtt catgtcgtac cgccagccga
     2341 tcgccgtgta ggggacgcgg aagtacttcg cgacgccgag gaggatcttt ccccaccact
     2401 tgtcctcggg aacgcccacg gcggcgagga tctcgcgcag gttcaggccg atctcgatcc
     2461 cgatctcgcg ccggcggttc tccgggggcg agtaccggta gcccttggac tggtacgtcc
     2521 cggagaggag gaggaagcgc ccgaggccgg acttcgttcc catccgcggg aggaggcccg
     2581 cgagcttgag atcgagcgtg tagatctcct tggagtagtc gtcgccatag ccgccgtacc
     2641 ggcagcacgc ctcgggaatc ggcgtggaca tgaggccgaa ccggagcccg accgtgtcgt
     2701 cgagcttcca cgcgtcgatc ccgtacgcga cggccgcgcc gatcgagttc gccgcgatgt
     2761 cctcccacgc gaacccgtac ttcgagtagc cgtccccgag ctcgatgagc gctcccgtga
     2821 cggtcacgac cgcgagagac agcgcccgcg cctggcccgg cgtcttgccg agcgaacggt
     2881 acgccccctg caggacctgc gagcccaggt agccccagaa gatgtgcgac gccttgtcgg
     2941 cgccgcccgc gtaggtgtcc tccccgaacc agccctcgtg ggcgacgacg aacgtggatc
     3001 ggctcgagtc cttccaccac gcgaagtacg cgagaagcgg cgcgccgacg acgacgcccg
     3061 ccgtcagggt cgtcgtccgg gcgtcccaga accgcggggg cgcgggcggc gggggcgcga
     3121 tctccgcgtc cgggtgcgcg gacgcgcccg ccg
//
LOCUS       DAOMFY010000374         3114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_13204_length_3114_cov_10.720170, whole genome shotgun
            sequence.
ACCESSION   DAOMFY010000374 DAOMFY010000000
VERSION     DAOMFY010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13204_length_3114_cov_10.720170"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLB01_18795"
     CDS             <1..1103
                     /locus_tag="PLB01_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79403.1"
                     /translation="LLARVQQAKADIIDALEGYRFDLAAKTPHEIVFTIDTQPARDVL
                     GLLAGAPGPTAALRRLKASTHATAAIRAQNLSPDDYYGKLVSAIAGTPDPLFTSFIGT
                     SPTFTALLDEMDATGSADAALLARRLASLIPAGMPVKVHVVLVPFLAISGFAEIVSVS
                     GDGEIVISADLPRIVGSPATAPQPREALLKVLRGAAAEAWRTLFEAQVRRAPAWPPAG
                     DKGLDFDALLARTVAEGPGTLFLVPDEFFPLSPFLEEPIVRSFGRWNTAAELLLEGKV
                     KEDRRLDLFLNAGKGDFWQRYPAIVGAQMTDGVLRLAGREKFLEALAAGPRAVAALYV
                     SATKGKGYPEFSKLVKKALEEKVRPSGAGEKR"
     gene            complement(1072..1425)
                     /locus_tag="PLB01_18800"
     CDS             complement(1072..1425)
                     /locus_tag="PLB01_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HQQ79404.1"
                     /translation="MAVIPKDVQVLGDELAIVWADGAETYLKLADLRRLCPCASCQGE
                     GDLLGRISKPPAKPLTRESYVVKGTSPVGGYALQIFWGDGHADGLYTYARLKAWGENP
                     PDLPPVSAFPLLPTV"
     gene            complement(1460..2734)
                     /locus_tag="PLB01_18805"
     CDS             complement(1460..2734)
                     /locus_tag="PLB01_18805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ79405.1"
                     /translation="MIARVLDGFRRVLAENLRFALIALRAHKLRAFLTMVGIVIGVWT
                     VIGMVSLVTGFDRSQTEAFSSFGTTLVQFQKFEPRFGPGHRSEEERKRRDLTIEDAQA
                     LLDLCPSILAVSPERYMWTPSKVKANGVEANSPQIGGVNQSYAICNNWEIKDGRNITE
                     TDVLHATDVAVIGQDVIESLFKGKDPLGQILTINGKRVQVVGTFEKKGSSGFGGQPDN
                     IVVIPISTFDRHFPQTKNSHDDTIHIATIPRSPELVDRVIEEGTAVLRARRKVPFNAP
                     NDFAIFTAEKMLAQMQAVTNAISGVMVLIAAIALLVGGVGVMNIMLVSVTERTKEIGL
                     RKSVGALRRDITLQFLTEAMTLTALGGAVGVLLGLLTSLVVKNVSSLRTETPLWSILL
                     GLGVSVSIGLFFGIYPAMKAARLNPIDALRYE"
     gene            complement(2731..>3114)
                     /locus_tag="PLB01_18810"
     CDS             complement(2731..>3114)
                     /locus_tag="PLB01_18810"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQQ79406.1"
                     /translation="AFALLVFISGISLVVGAIVIANIMLVSVIERTKEIGLRLAVGAR
                     KRDVLFQFLLEAAVLALGGGVLGVFFGWVIAICVDAFTPLPTLVTPGLVISGLVLATL
                     TGVIAGVFPAIKASRLTPIEALRFE"
BASE COUNT          504 a         1054 c         1071 g          485 t
ORIGIN      
        1 ggctcctcgc ccgcgtgcag caggcgaagg cggacatcat cgacgcgctc gagggttatc
       61 gcttcgacct cgccgcgaag acgccgcacg agatcgtctt caccatcgac acgcagccgg
      121 cgcgggacgt gctcgggctc ctcgccggcg cgcccgggcc gacggccgcg ctccggcgcc
      181 tgaaggcgtc gacgcacgcc accgcggcga ttcgcgcgca gaacctctcg cccgacgact
      241 actacggcaa gctcgtctcg gcgatcgcgg ggaccccgga tcctctcttc acgtcgttca
      301 tcgggacgag cccgaccttc acggcgcttc tggacgagat ggacgccacg ggctccgccg
      361 acgccgcgct cctggcgcgg cggctcgcat cgcttatccc cgcgggcatg ccggtgaaag
      421 tgcacgtcgt cctcgtgccg ttcctcgcga tctcgggttt cgccgagatc gtctccgtga
      481 gcggcgacgg cgagatcgtc atctcggccg accttccccg catcgtcggc agccccgcca
      541 cggcgccgca gcctcgtgag gccctcctga aagtcctgcg gggcgcggcg gccgaggcgt
      601 ggcgcaccct gttcgaggcg caggtgcgga gggcgcccgc ctggccgccc gcgggcgaca
      661 aggggctcga cttcgacgcg ctgctcgcgc gcaccgtcgc cgagggcccc ggcacgctct
      721 tcctcgtgcc ggacgagttc ttcccgcttt cgccattcct cgaggagccc atcgtgcgca
      781 gcttcgggcg ctggaacacg gcggcggaac tcctcctcga aggcaaggtg aaggaggacc
      841 gaaggctgga tctctttctg aacgccggca agggcgactt ctggcagcgc taccccgcga
      901 tcgtcggcgc ccagatgacg gacggcgtcc tgcgcctcgc ggggcgcgag aagttcctcg
      961 aggccctcgc ggcgggcccc cgcgcggtcg ctgcgctcta cgtctcggcg acgaagggca
     1021 agggctaccc cgagttttcg aagctggtga agaaggctct cgaggagaag gtcagaccgt
     1081 cgggagcagg ggaaaagcgc tgacgggcgg caggtccggg gggttttcgc cccaggcctt
     1141 cagccgcgcg tacgtgtaca gcccgtcggc gtgcccgtcg ccccagaaga tctggagcgc
     1201 gtagccgcct acgggcgatg tgcctttgac gacatacgac tcccgcgtga gcggtttggc
     1261 gggcggcttc gagatccttc cgaggagatc cccctctccc tggcacgagg cgcacgggca
     1321 gaggcgccgg aggtcggcga gtttcaggta cgtctcggct ccgtcggccc acacgatcgc
     1381 gagctcgtcg ccgaggacct gcacgtcttt cgggatcacg gccacgggcg gatcctaacc
     1441 ggaacgccgc gctccgcggc tactcgtagc gcagggcgtc gatcggattc aggcgcgcgg
     1501 ctttcatcgc agggtagatc ccgaagaaga ggccgatcga caccgagacc ccgaggccga
     1561 ggaggatgct ccagagcggc gtctccgtcc tgaggctcga gacgttcttg acgacgagcg
     1621 acgtcagaag gccgaggagg acgccgacgg cgcccccgag ggccgtgagc gtcatcgcct
     1681 ccgtgaggaa ctggagagtg atgtccctcc ggagcgcgcc gacggacttc ctcaggccga
     1741 tctccttcgt ccgctccgtg acggagacga gcatgatgtt catgacgccg acgccgccca
     1801 cgaggagcgc gatcgccgcg atgaggacca tgacgccgga gatcgcgttc gtcacggcct
     1861 gcatctgggc gagcatcttc tcggcggtga agatcgcgaa gtcgttcggg gcgttgaacg
     1921 gcaccttgcg ccgcgcccga aggacggctg tgccctcctc gatgacgcga tccacgagct
     1981 cgggcgagcg cggaatcgtc gcgatgtgga tcgtgtcgtc gtgggagttc ttcgtctgcg
     2041 ggaaatggcg gtcgaacgtc gagatcggga tcacgacgat gttgtcgggc tgcccgccga
     2101 agcccgacga gcccttcttc tcgaacgtcc cgacgacctg cacgcgcttg ccgttgatcg
     2161 tgaggatctg cccgagcggg tccttgccct tgaagagcga ctcgatgacg tcctgaccga
     2221 tgacggcgac gtccgtcgcg tggaggacgt ccgtctccgt gatgttgcgg ccgtccttga
     2281 tctcccagtt gttgcagatc gcgtagctct ggttcacgcc gccgatctgc ggggagttcg
     2341 cctcgacgcc gttcgccttc accttggacg gggtccacat gtaccgctcg ggcgacacag
     2401 cgaggatcga ggggcacagg tcgaggagcg cctgcgcgtc ctcgatcgtg aggtcgcgcc
     2461 ttttgcgctc ctcctcggag cggtggccgg ggccgaaacg cggctcgaac ttctggaact
     2521 ggacgagcgt cgtcccgaac gacgagaaag cctcggtctg cgagcggtcg aatcccgtca
     2581 cgagcgaaac cattccgatg acggtccaga cgccgatcac gatgccgacc atcgtgagaa
     2641 atgcgcgcag cttgtgggcg cgcagcgcga tcagcgcgaa ccgaaggttc tccgcgagga
     2701 cgcgccggaa cccgtcgagg acgcgcgcga tcattcgaac ctcagggctt cgatcggcgt
     2761 caggcgcgag gccttgatcg ccggaaagac gcccgcgatg acgcccgtga gcgtggcgag
     2821 aacgaggccc gagatgacga ggccgggcgt cacgagcgtc gggagcggcg tgaaggcgtc
     2881 cacgcagatc gcgatgaccc agccgaagaa caccccgagg accccgccgc ccagtgcgag
     2941 gaccgccgcc tcgagaagga actggaagag aacgtcgcgc ttgcgcgctc cgaccgcgag
     3001 cctcagcccg atctccttcg tccgctcgat gaccgagacg agcatgatgt tcgcgatcac
     3061 gatcgcgccg acgacgagcg agatcccgct gatgaagacg aggagcgcga acgc
//
LOCUS       DAOMFY010000375         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_13476_length_3079_cov_8.516204, whole genome shotgun sequence.
ACCESSION   DAOMFY010000375 DAOMFY010000000
VERSION     DAOMFY010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13476_length_3079_cov_8.516204"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PLB01_18815"
     CDS             complement(<1..1373)
                     /locus_tag="PLB01_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79407.1"
                     /translation="MKSPLAASCLLAAACLAPPARAADGGLEAVETVSTPGRFDKYPS
                     VAFDASGDLWCAFTTTDGANDRVVVRRRKDGVWSDEERLDAGEGLESAAMLVVDGKKR
                     LRAFWHGKRKGEWAVYSRTRTGGAWGPEKRISSKGSDALHPVVTLDSSRRLWVAWEEF
                     KRGGFAVSLVAENGSGWSAPVRVFTSGTDRRPALAAPPDGGVWLAWDSTHTGNYDVFL
                     ARARASGSRAPSLGAPIAVTSDAAIDDSPSLACTKDGALWIAWNSMRGRSTDEFRADR
                     HSGDAFVRVYRDGAFFAPPGTAPAALPGQVSFGSVDKSLRDVEEPYWHWKQTQNYPSV
                     FLDGAGRAWIVWRTDATGAHNFDLWARVHDGRRWSPELHLTTFSPGRDEFPSAALAPD
                     GSLAFAWEAQTLPKAGEALTGGDVDAYNTHSSPNVVLAGRLAAPAEGWAPGALGGARQ
                     LLRLVAQG"
     gene            1428..2261
                     /locus_tag="PLB01_18820"
     CDS             1428..2261
                     /locus_tag="PLB01_18820"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79408.1"
                     /translation="MAAALLLALKLSVAALIFAIGLGSTTADFGYLWRRPALLVRSLL
                     AMYVVVPLAALAAVRLLDLPLPVKAAILVLAISAGAPLLPKKLMKLGREGYVFSLVVT
                     SSLLAIVTVPAWLTVLGPLFGRESTVDPREVAFLIAKAFLGPLVLGMIVRRLAGPRAE
                     RASDWILKVAGATLLAAGLVLLVGARHLLVAVGWVPLASLAGMTAVALAIGHALGGPD
                     PDDRTALAIACATRHVGIAMLAASTVPGPRTIAFVLAYILASAAVSIPYLRWRARRPG
                     I"
     gene            2304..2729
                     /locus_tag="PLB01_18825"
     CDS             2304..2729
                     /locus_tag="PLB01_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQQ79409.1"
                     /translation="MALPQVESVRVPFGEVAGRLKTALESAPPSVVAAYLFGSVARGE
                     AREGSDVDVAVLFSAPTEPILGNAANHLECDLERVLRRTVQVVELNKANADLVHRVLR
                     DGVILLDRDRGARIEFEVRLRNEYFDLEPIRRMYRGTAS"
     gene            2726..>3079
                     /locus_tag="PLB01_18830"
     CDS             2726..>3079
                     /locus_tag="PLB01_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HQQ79410.1"
                     /translation="MTDPQLVAKKLAVVETSVRELRTLARPAELRRDVRERRFVEHTL
                     QVAIQAVLDVASHIVSDRRLGEPQTNRELFDLLAKAGFIEERMAAPLREMAGFRNILV
                     HGYQDVDLVIVETILR"
BASE COUNT          412 a         1138 c         1071 g          458 t
ORIGIN      
        1 ccctgcgcga ccagacgcag gagctgtcgg gcgccgccga gcgcgccggg cgcccagccc
       61 tcggcgggcg ccgcgagccg tccggcgagg acgacgttcg ggctggagtg cgtgttgtac
      121 gcgtccacgt ccccgcccgt cagcgcctcg cccgccttcg gcagcgtctg cgcttcccag
      181 gcgaaggcga gcgacccgtc gggcgcgagc gccgccgacg ggaactcgtc gcggccgggc
      241 gagaacgtcg tgagatgcag ctccggcgac cagcgccgcc cgtcgtggac gcgcgcccag
      301 aggtcgaagt tgtgcgctcc cgtcgcgtcc gtccgccaca cgatccacgc gcgccccgcg
      361 ccgtcgagga agaccgacgg gtagttctgc gtctgcttcc agtgccagta gggctcctcg
      421 acgtcgcgca gggacttgtc caccgaaccg aagctcacct ggcccggcag cgcggccggc
      481 gccgtgcccg gcggagcgaa gaaggccccg tcccggtaaa cgcggacgaa cgcatcaccc
      541 gagtggcggt ccgcccggaa ctcgtccgtc gagcggccgc gcatcgagtt ccacgcgatc
      601 cagagcgcac cgtctttcgt gcacgcgagg gacggcgagt cgtcgatcgc cgcgtcggac
      661 gtgacggcga tgggcgcgcc gaggctcggc gcgcgggaac cggaggcccg cgcccgcgcg
      721 aggaagacgt cgtagttgcc ggtgtgggtc gagtcccagg ccagccacac gcctccgtcc
      781 ggcggcgcgg cgagcgcggg acgccggtcc gtcccggagg tgaagacgcg aaccggcgcg
      841 ctccagcccg acccgttctc cgccaccagc gagaccgcga acccgccgcg tttgaactcc
      901 tcccacgcca cccagagccg acgagaggag tcgagcgtca cgaccggatg taaggcgtcc
      961 gaacccttcg aagaaatcct cttctccggt ccccatgcgc cgccggttct cgtgcgcgaa
     1021 tagacggccc actctccctt tctctttcca tgccagaagg cccgcaacct cttcttcccg
     1081 tcgaccacga gcatcgctgc cgactcgaga ccctccccgg catcgaggcg ctcttcgtcc
     1141 gaccagacgc cgtcctttcg ccgccggacg accacgcggt cgttcgcgcc gtccgtcgtc
     1201 gtgaacgcgc accagaggtc gcccgaggcg tcgaacgcga cggacgggta cttgtcgaat
     1261 cgccccggtg tcgagaccgt ctcgacggcc tcgaggccgc cgtcggccgc gcgggccgga
     1321 ggcgcgagac acgcggcggc aagaaggcag gatgcggcga gcggcgattt catggtgggg
     1381 aagtctaggg gacggcggcc ggcgcggcgc gataacctcg agtgagcatg gcggccgcgc
     1441 tcctcctcgc cctcaagctc tcggtcgccg cgctgatctt cgcgatcggg ctcggctcga
     1501 cgaccgcgga cttcgggtac ctctggcggc ggcccgcgct gctcgtccgc tcgctcctcg
     1561 cgatgtacgt cgtcgtgccg ctcgcggctc tcgcggccgt ccggctgctc gacctgccgc
     1621 tgccggtcaa ggccgcgatc ctcgtcctcg cgatctcggc cggcgcgccg ctcctcccga
     1681 agaagctgat gaagctgggc cgggaaggct acgtcttcag cctcgtcgtc acgtcgtcgc
     1741 ttctcgccat cgtgaccgtg cccgcctggc tgacggtcct cggcccgctc ttcgggcgcg
     1801 aatcgaccgt ggacccccgc gaagtcgcct tcctgatcgc gaaggccttc ctcgggccgc
     1861 tggtcctcgg catgatcgtc cgccggctcg ccgggccgcg ggccgagcgc gcgtccgact
     1921 ggatcctgaa ggtcgcgggc gcgacgctcc tcgcggccgg gctcgtcctt ctcgtcgggg
     1981 cgagacacct gctcgtcgcg gtgggctggg tcccgctcgc ctcgctcgcg ggaatgaccg
     2041 cggtggcgct cgcgatcggg cacgcgctgg gcgggcccga tcccgacgac cgaacggcgc
     2101 tcgccatcgc gtgcgcgacc cggcacgtcg ggatcgcgat gctcgccgcg tcgaccgtgc
     2161 ccggcccccg gacgatcgcg ttcgtcctcg cctacatcct cgcctcggca gccgtctcga
     2221 ttccgtacct cagatggaga gcgcggcggc cggggatctg aggagcgtgc cccggtcatc
     2281 gcccgcgaag ctagaatcgc cgtgtggcgc tgccccaggt cgaatccgtc cgggttcctt
     2341 tcggcgaggt cgccgggcgg ctgaagaccg cgctcgagtc cgccccgccc tcggtcgtgg
     2401 cggcgtacct gttcggcagc gtggcacgcg gcgaggcgcg agagggcagc gacgtggacg
     2461 tggcggtcct cttctcggcg ccgaccgagc ccatccttgg gaacgcggcc aaccatctcg
     2521 aatgcgacct cgagcgcgtc ctcaggcgga ccgttcaggt cgtcgagctg aacaaggcga
     2581 atgcggacct tgtccaccgc gtcctgcgcg acggcgtgat cctcctcgac cgcgaccgcg
     2641 gagcgcgcat cgagttcgag gtccgcctgc ggaacgagta cttcgacctc gagccgatcc
     2701 gccgcatgta ccgcgggacg gcgtcgtgac ggacccgcag ctcgtcgcga agaagctggc
     2761 cgtggtggag acgtcggtcc gcgagttgcg gacgctcgcg cggcctgccg agctgcggcg
     2821 ggacgtccgc gagcggcggt tcgtggagca cacgctgcag gtggcgattc aggcggtcct
     2881 agacgtcgcg tcccacatcg tctcggaccg gcggctcggc gagccgcaga cgaaccgcga
     2941 gctcttcgac ctcctggcga aggccggatt catcgaggag cggatggccg ctccgctgcg
     3001 ggagatggcc ggcttccgga acatcctcgt tcacggctac caggacgtgg acctcgtgat
     3061 cgtcgagacc atcctcagg
//
LOCUS       DAOMFY010000376         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_13643_length_3055_cov_4.476667, whole genome shotgun sequence.
ACCESSION   DAOMFY010000376 DAOMFY010000000
VERSION     DAOMFY010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13643_length_3055_cov_4.476667"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLB01_18835"
     CDS             <1..937
                     /locus_tag="PLB01_18835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ79411.1"
                     /translation="GNLHAGLERVDALEKDRDARFEALARERERDSTEREESGRRLDE
                     ARESLRMLRLEWSSLRSNLQSVAGGAALPDARGSEANRPLSGGGALRADDPLRAGLYV
                     DFEEHFRGSEDEIRRRQKIDAARFRGAPGPVADLGCGRGEFLEALAAEGVEGVGCDAN
                     PVMAARAKEKNLAVDAADLFEWLAARPDGSLGGITAFQVVEHLPPASLFDLVELAARK
                     LAAGGRVLFETVNPESVYAMRWFWMDLTHVRPVPAPSLAQLLRANGFRDVAVDFRSPV
                     PASEGLSEEAAKDPRLAPVARLLFAPQDYAVTGVK"
     gene            946..1983
                     /locus_tag="PLB01_18840"
     CDS             946..1983
                     /locus_tag="PLB01_18840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQQ79412.1"
                     /translation="MTRILVCNAQVPFASGGAERHADGLVRELLAAGHEAELVRLPFK
                     WYPREEILTSALAWRLLDVSEADGRRVDLVIPMKFPSYLVRHENKVVWLIHQFRQAYD
                     RFGTPESDFTASPADSRWRELIADADRTGLGEARKVFTNAKNTADRLARFNGIAAEAL
                     YHPPPLAGRYRCEASAGFALAAGRLDSWKRMDLAIGAAAAGKFRLVIAGDGPDRARLE
                     AIAKKSGADVRFAGRVSDEELLSLYATCGAVLFPPADEDYGYIALEAFLSKKPVVTCT
                     DSGGPLEFVVEGENGRVVAPDAAAIAEATTGLLADAKKSGEFGGRGFERVRGISWSHA
                     VRSLLEAGGIR"
     gene            1980..>3055
                     /locus_tag="PLB01_18845"
     CDS             1980..>3055
                     /locus_tag="PLB01_18845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ79413.1"
                     /translation="MTPLSVGFVGPLPPTRSGIADYDAEILSSLSKKSRLSLVSYEPD
                     QAERAVRAGHHVLLFQIGNDPLHAPSVEALCAPGRTTPAVVVLHDFVLHHLFAAAYLD
                     RGREADYARALEAAHGVRGRAFAEEGRRGPRIPVWDLDPWAFPMSGAVVRAAEAVVAH
                     SALVRGAVLRESPKTRVAEIPHHVVEAPRTPRDEARRALGLPLDRPVAASLGVVTPAK
                     RIGKILEALATLPAGRRPFLFVGGAVGEDDPLHRYVDDHGLGGDVAFGGYLSDDDFWR
                     AASAADFGVNLRHPTMGETSGAVCRLAGFGLPLVVSDTGWFRELPDAFASKVPVGGDE
                     VPRLAAEMEALAFDPERTRARAAAA"
BASE COUNT          402 a         1096 c         1113 g          444 t
ORIGIN      
        1 ggggaacctc catgcgggcc tcgagcgcgt cgacgcgctc gagaaggacc gcgacgcgcg
       61 cttcgaggcg ctcgcccgcg agagagaaag agattctact gaaagggaag agagcgggcg
      121 gcggctcgac gaggcgcgcg agtcgttgcg catgctgcgt ctcgaatggt cttcccttcg
      181 aagcaatctg cagagcgtcg ccggtggcgc ggcgctgcca gacgcgcgag ggtccgaagc
      241 aaaccgccct ctctccgggg gcggcgcact ccgcgccgac gacccgctcc gggcggggct
      301 ctacgtggac ttcgaggagc atttccgggg cagtgaggac gagatccggc gccgccagaa
      361 gatcgacgcc gctcgcttcc ggggagcgcc cggccccgtc gccgacctcg gctgcggccg
      421 cggcgagttc ctggaggcgc tcgcggccga gggcgtggag ggcgtcggct gcgacgcgaa
      481 ccccgtcatg gccgcccgcg cgaaggagaa gaatctcgcc gtggacgctg ccgacctctt
      541 cgagtggctc gcggcccggc ccgacggctc gctgggcggg atcacggcgt tccaggtcgt
      601 ggagcacctg ccgcccgcgt cgctgttcga tctcgtcgag ctcgcggcgc gcaagctcgc
      661 ggccggcggg cgcgtcctgt tcgagacggt gaaccccgag tcggtctacg ccatgcgctg
      721 gttctggatg gacctcaccc acgtgcggcc cgtgccggcg ccgtcgctcg ctcagctgct
      781 ccgggcgaac gggtttcggg acgtcgccgt ggacttccgt tcgccggtcc ccgcgagcga
      841 ggggctttcc gaggaggccg cgaaggaccc gaggctcgcg cctgtcgcgc ggctcctctt
      901 cgcgccgcag gactacgccg tcacgggcgt gaagtaggcc cgcgcatgac ccgcatcctc
      961 gtctgcaacg ctcaggtgcc gttcgcctcg ggcggcgccg agcggcacgc ggacgggctc
     1021 gtgcgcgagc tcctcgcggc ggggcacgag gccgagctcg tacggctgcc gttcaagtgg
     1081 tacccgcggg aggagatcct gacgtccgcg ctcgcgtggc gccttctcga cgtctccgag
     1141 gccgacggcc ggcgcgtcga cctcgtgatc ccgatgaagt ttccctcgta cctcgtgagg
     1201 cacgagaaca aggtcgtctg gctcatccac cagtttcgcc aggcatacga ccggttcggg
     1261 acgcccgaga gcgacttcac cgcgtcgccc gcggactccc ggtggcgcga gctgatcgcg
     1321 gacgcggacc ggacggggct cggcgaggcg cggaaggtct tcacgaacgc gaagaacacg
     1381 gccgaccggc tcgcgcggtt caacgggatc gccgccgagg cgctctacca cccgccgccg
     1441 ctcgcgggcc ggtaccggtg cgaggcgtcg gcggggttcg cgctcgcggc cggccgactc
     1501 gactcgtgga agcggatgga cctcgcgatc ggcgcggcgg cggccgggaa gttccgcctc
     1561 gtgatcgcgg gcgacggccc ggaccgcgcg cgactcgagg cgatcgcgaa gaagagcggc
     1621 gcggacgtga ggttcgcggg acgcgtgtcg gacgaagagc tcctttcgct ctacgcgacc
     1681 tgcggcgcgg tcctcttccc gcccgccgac gaggactacg gctacatcgc gctcgaggcg
     1741 ttcctctcga agaagcccgt cgtgacgtgc acggattccg gcggccccct ggagttcgtc
     1801 gtagaaggcg agaacgggcg ggtggtggcc ccggatgccg ctgcgatcgc cgaagcgacg
     1861 accggattgc tcgcagacgc gaagaaatca ggggaattcg gaggaagggg ctttgaaagg
     1921 gtaagaggga tcagctggag ccatgcggtc cggtccctcc tcgaggcggg cgggattcga
     1981 tgacccctct ctccgtcgga ttcgtcggcc cgcttccccc gacgcgctcc ggcatcgcgg
     2041 actacgacgc cgaaatcctc tcttcacttt cgaagaaatc ccgcctttct ctcgtctcct
     2101 acgagccgga ccaggccgaa agagccgttc gggccggcca ccacgtcctc ctcttccaga
     2161 tcgggaacga cccgctgcac gcgccgtccg tcgaggcgct gtgcgcgccc gggcggacga
     2221 cgcccgccgt cgtcgtcctg cacgatttcg tcctgcacca tctcttcgcc gcggcgtacc
     2281 tcgaccgggg ccgcgaggcg gactacgccc gggccctcga ggccgcacac ggcgtgcggg
     2341 gccgcgcgtt cgccgaggag gggcggcgcg gcccccggat tccggtgtgg gacctcgacc
     2401 cgtgggcctt cccgatgtcg ggtgccgtcg tccgcgccgc cgaggcggtc gtcgcgcact
     2461 cggcgctcgt ccggggcgcc gtcctgcgcg agagcccgaa gacgcgcgtg gccgagattc
     2521 cccaccacgt cgtcgaggcg ccgcgcacgc cccgcgacga ggcgcgccgc gcgctcggcc
     2581 tgccgctcga ccgaccggtc gcggcgtctc tcggcgtcgt gacccccgcg aagaggatcg
     2641 ggaagatcct cgaggcgctc gccaccctcc cggccgggcg ccggccgttc ctcttcgtcg
     2701 gcggcgccgt cggagaggac gacccgctcc accgatacgt cgacgatcac gggctcggcg
     2761 gcgacgtcgc gttcggcggg tacctctcgg acgacgactt ctggcgcgcg gcttcggcgg
     2821 cggacttcgg ggtgaacctc cggcacccga cgatggggga gacgtccgga gcggtctgcc
     2881 ggctcgccgg cttcggcctg ccgctcgtcg tctccgacac gggctggttc cgcgagcttc
     2941 cggacgcgtt cgcttcgaag gttccggtcg gcggcgacga ggtcccgcgc ctcgcggccg
     3001 agatggaggc gctcgcgttc gatcccgagc gcacccgcgc gcgcgcggcg gccgc
//
LOCUS       DAOMFY010000377         3045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_13709_length_3045_cov_5.800334, whole genome shotgun sequence.
ACCESSION   DAOMFY010000377 DAOMFY010000000
VERSION     DAOMFY010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13709_length_3045_cov_5.800334"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1452)
                     /locus_tag="PLB01_18850"
     CDS             complement(52..1452)
                     /locus_tag="PLB01_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ79414.1"
                     /translation="MSAARVLVCDDEPGIRETLGQILGDEGYVVDAVGLGQEAVRRAL
                     GDAPPDAVLLDVWLPDGDGLTVLDRLRAGGFAGPVVMISGHGTVETAVGAVKRGADDF
                     LEKPLALERVLLTLEKALERTRLARERDDLRRELASRDARAAEEELLLGDSPAMARLK
                     SEIARAGASDARVLITGESGTGKELVARALHRASPRAAGPFVEVNGAAIPEELIESEL
                     FGHVKGSFTGAFEDRKGKWEQADGGTLFLDEIGDMSPRTQAKILRAIQDGKITRVGGT
                     KTVATDVRIVAATNRDLPAMIRAGAFREDLYFRLAVVPLVVPPLAERPEDVPLLVAHF
                     AESVARKRGRRAKTFEPAALAALAARRWPGNVRELQNLVERVLLMTSGPSVRLQDLPP
                     EEGAPRPVSGSHAVSDISGVLPLAEARDAFEKKYLARVLAETKGNVSRAAERLGLDRT
                     TLHRKMKSLGLDGDKD"
     gene            complement(1460..>3045)
                     /locus_tag="PLB01_18855"
     CDS             complement(1460..>3045)
                     /locus_tag="PLB01_18855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ79415.1"
                     /translation="GGPPVAVSSPRWPVRDVADAGADWLADAKARGVSRRIEATHDGG
                     QTGRSLFVSPAGTLLVGTFEPSAEAEPIRELGRATSAYAMLKAERASLEAIQVLLFLL
                     LAFVVLLAAVWVGLLLARRVTRPIGALAASARRVGAGDFDALVEVEGGDEIGALARAF
                     NAMTSELRRRRAELVAANAELEATNRQLDEQRRRFGSILERLDAGVVAFWEDGRLAFL
                     NDTARRLLDREGEGAVERLDDLLAPAPLAPLREFLSRAREGGLREATLKIGRRVLEAH
                     LAQAPADSGGAGPSIVTLEDTTALVAAERAAAWEEAARRMAHEIKNPLTPIRLAAERM
                     RRRAASSPDSGDGLPRVVEEGASTIVQEVAALAALVDTFQRFARLPAAVLAPTDLAAV
                     ARQVAKLYDGVKRGIAVTAEAPEDLAPARADAGQLRRALINLVDNAVAATPEGGRVRV
                     AVRVVEGRAVVSVEDDGPGIAPADRERVFDAEYSTKGRGSGLGLAIVARIAAEHGGTV
                     RVEENAPHGCRFLLEWPAA"
BASE COUNT          391 a         1118 c         1149 g          387 t
ORIGIN      
        1 aacagcattc caccggttaa cccgggtgaa caacctcctg agtctttaca cctagtcctt
       61 gtcgccgtcg aggccgaggg acttcatctt gcggtggagc gtcgtgcggt cgaggccgag
      121 gcgctcggcc gcgcgcgaga cgttgccctt cgtctccgcg aggacgcgcg cgaggtactt
      181 cttctcgaac gcgtcgcgcg cctcggcgag cgggagcacg ccggagatgt ccgacacggc
      241 gtgcgacccg gagacggggc gcggcgcacc ctcctcgggc ggaagatcct ggagccgcac
      301 ggacgggccg gacgtcatga ggaggacgcg ttcgacgaga ttctggagct cgcggacgtt
      361 gccgggccag cgccgcgccg cgagcgccgc gagcgccgcg ggctcgaacg tcttcgcgcg
      421 ccgcccgcgc ttccgggcga ccgactcggc gaaatgcgcc acgaggagcg ggacgtcctc
      481 gggccgctcc gcgagcggcg gcaccaccag cgggacgacc gcgaggcgga agtagaggtc
      541 ctcgcggaag gcgcccgcgc ggatcatcgc cgggaggtcg cggttcgtag ccgcgacgat
      601 ccgcacgtcg gtcgcgacgg tcttcgtgcc gccgacacgc gtgatcttcc cgtcctggat
      661 cgcgcggagg atcttcgcct gggtccgcgg tgacatgtcg ccgatctcgt cgaggaagag
      721 cgtcccgccg tcggcctgct cccacttgcc cttgcggtcc tcgaacgcgc ccgtgaacga
      781 ccccttcacg tggccgaaga gctcgctctc gatgagttcc tcgggaatcg cggcgccgtt
      841 gacctcgacg aacgggcccg cggcgcgcgg cgaggcgcgg tggagcgccc gcgcgacgag
      901 ttccttgccg gtcccgctct cgcccgtgat gagaacgcgc gcatccgagg ctcccgcccg
      961 cgcgatctcg gacttcaggc gcgccatcgc agggctgtcg ccgaggagga gctcctcttc
     1021 cgcggcacgt gcgtcgcggc tcgcgagttc gcgcctgagg tcgtcgcgct cgcgcgcgag
     1081 ccgcgtgcgc tcgagggcct tctcgagcgt caggaggacg cgctcgagcg cgagaggctt
     1141 ttcgaggaag tcgtccgcgc cgcgcttgac ggccccgacg gccgtctcga cggttccgtg
     1201 gcccgagatc atgacgacgg ggccggcgaa gcctcccgcg cggaggcggt cgaggaccgt
     1261 gaggccgtcc ccgtcgggca gccagacgtc gaggaggacg gcgtcgggcg gcgcgtcgcc
     1321 gagcgcgcgc cggaccgcct cctgcccgag gccgactgcg tccacgacgt atccctcgtc
     1381 gccgaggatc tggccgagcg tctcgcggat gccgggctcg tcgtcgcaga cgaggacgcg
     1441 cgccgcgctc acgcgcgcct cacgctgcgg gccactcgag gaggaatcgg catccgtggg
     1501 gtgcattctc ctccacgcgc accgtgccgc cgtgctcggc cgcgatccgc gcgacgatgg
     1561 cgagcccgag gcccgaaccc cggcccttcg tcgaatactc ggcgtcgaag acgcgctcgc
     1621 ggtccgcggg ggcgatcccc gggccgtcgt cctcgaccga cacgaccgcc cgcccctcca
     1681 cgacccgcac ggccacccgg acgcgcccgc cctcgggcgt tgcggcgacc gcgttgtcga
     1741 cgaggttgat gagcgcacgg cggagctgtc ccgcgtcggc ccgcgcgggc gcgaggtcct
     1801 ccggagcctc ggccgtcacg gcgatcccgc gcttgacgcc gtcgtagagc tttgcgacct
     1861 ggcgggccac ggcggcgagg tccgtggggg cgaggaccgc cgcaggcagg cgcgcgaaac
     1921 gctggaacgt gtccacgagc gcggcgagcg cggccacctc ctggacgatc gtcgacgccc
     1981 cctcctcgac gacgcggggg aggccgtcgc cggagtcggg agaggacgcc gcgcggcggc
     2041 gcatgcgctc ggccgcgagt cggatcggcg tgagcgggtt cttgatctcg tgggccatgc
     2101 ggcgcgcggc ctcttcccac gcggccgcgc gctcggccgc gacgagggcg gtcgtgtcct
     2161 cgagcgtcac gatgctcggg cccgcgccgc cggagtcggc cggggcctgc gcgaggtgcg
     2221 cctccagaac gcgccggccg atcttgagcg tcgcctcgcg cagtccaccc tcgcgcgcgc
     2281 gggagaggaa ctcgcgcagc ggggcgagcg gagcgggcgc gaggaggtcg tcgagccgct
     2341 cgacggcgcc ctcgccctcg cggtccagga gacggcgcgc cgtgtcgttc aggaacgcga
     2401 ggcggccgtc ctcccagaac gcgacgacgc cggcgtccag ccgttcgagg atcgagccga
     2461 accgccggcg ctgctcgtcg agctgccggt tcgtggcctc gagctccgcg ttcgcggcga
     2521 cgagctcggc cctgcggcgt ctcagctcgg aggtcatcgc gttgaacgcg cgcgcgaggg
     2581 cgccgatctc gtccccgccc tccacctcga cgagcgcgtc gaagtcgccc gccccgacgc
     2641 ggcgcgccga ggccgcgagc gccccgatcg gacgcgtcac gcgccgcgcg aggaggaggc
     2701 cgacccagac cgccgcgagg aggacgacga aggcgaggag gaggaagagg aggacctgga
     2761 tcgcttcgag cgaggcgcgc tcggccttca gcatcgcgta ggcggacgtc gcgcgaccca
     2821 gctcgcggat gggctcggcc tcggcggagg gttcgaacgt gccgacgagg agcgtgcccg
     2881 cgggcgagac gaagagcgac cggccggtct gcccgccgtc gtgcgtggcc tcgatccggc
     2941 gcgagacacc gcgcgccttc gcgtcggcca gccagtccgc cccggcgtcg gcgacgtcgc
     3001 ggaccggcca gcgcggcgag ctcacggcga cgggcgggcc gccgg
//
LOCUS       DAOMFY010000378         3007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_14020_length_3007_cov_7.654472, whole genome shotgun sequence.
ACCESSION   DAOMFY010000378 DAOMFY010000000
VERSION     DAOMFY010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14020_length_3007_cov_7.654472"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLB01_18860"
     CDS             complement(<1..225)
                     /locus_tag="PLB01_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79416.1"
                     /translation="MTVLLAACFFVSGAAGLAYEVLWSRHLALLFGANAEAVGLVLAV
                     FMSGLGLGSRAFGALADRARAPLRLYAALEA"
     gene            253..810
                     /locus_tag="PLB01_18865"
     CDS             253..810
                     /locus_tag="PLB01_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79417.1"
                     /translation="MKAKISAAVLFLAAAAAPAAVDAYMTFVTLPQAVKLGDGSVLPA
                     GKYDVKIDYKGFGNSAEFLFLQGGALKGKCPAEARGFPSQAPAGAADANAVKLHDVKI
                     TDPAAQKGYTVKTNPGTEKVFPKVETGDKVQPGAAAASFSWGAHGFAPGIVGKTAPAG
                     RSLKLSFDSANSAAGFSALLPAVQK"
     gene            complement(862..1668)
                     /locus_tag="PLB01_18870"
     CDS             complement(862..1668)
                     /locus_tag="PLB01_18870"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HQQ79418.1"
                     /translation="MKNPVLAMLAGAVLAAGPALAQFEGELTMKLTMREGNGTGKAYV
                     SSAGARSELDIQTAQMPFKMTTLMKASNPEIMYMINDEQKSYAEIDLKKMREQTAKYR
                     DKSKETWSVKKLGKETVNGYSSEHVLVTRAEDAKSEQELWTSKDISGLSYESMRGLMR
                     RGNQSDEGMMKALRDAGVDGFVVKMITREKGNPAPLATMELTKVERKALKAALFEIPA
                     GYAKKEGMMGAASVMAPPGANDEMRKAMEGLTPEQRKQIEEMMKRQQQSK"
     gene            complement(1730..>3007)
                     /locus_tag="PLB01_18875"
     CDS             complement(1730..>3007)
                     /locus_tag="PLB01_18875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQQ79419.1"
                     /translation="AVWARLAPGTRDGLNAIWGPLAVNAGAALDALFFASVGLLFFLA
                     FFHRRRLLAVGAVLAALFGTLMGVLPPPVGPFERSLMVGFGGIAVVLLVFAFSDLLAA
                     ATAAFGGGLLLSFLPLAAVASGPALAGPLMGLALPLGLLLGVSALGLASGRTIVYAYE
                     DLAPHVKRIVERERIKAEIDAANRIQAALLPSSHPELGSASLASSYRAATEIGGDYFD
                     FLPLPDGKLGLAFGDVAGHGLTSGIVMAMTKAALLVQVRHDVSPVRVLEVLNDIVMKT
                     APKRMLMTFFYGVLDPETSTLRFASAGHLDPYVFRAREKKLEMLSSWGFPLGVRRREP
                     FREFDVRFEPGDRLVLYSDGLIEALNDDGEPFGFERFEGVILSRATKGADVLKQALLE
                     SVKSFTRNRPPEDDQTLVVVSFEDRQVVALRAS"
BASE COUNT          484 a         1054 c         1017 g          452 t
ORIGIN      
        1 cgcttccagc gccgcgtaga gtctgagcgg cgcgcgggcc cggtcggcga gggccccgaa
       61 cgcgcggctg ccgaggccga gaccgctcat gaacacggcg aggacgagcc cgacggcttc
      121 cgcgttcgcg ccgaacagga gcgcgaggtg ccgcgaccac agcacctcgt acgcgagccc
      181 cgcggcgccc gaaacgaaga aacaggcggc gagaaggacg gtcataaaat ccgtctcaag
      241 aggaggctca ttatgaaggc caaaatctca gccgccgtcc tgttcctcgc ggccgccgcg
      301 gcgcccgcgg ccgtcgacgc gtacatgacg ttcgtcacgc tcccgcaggc cgtcaagctc
      361 ggcgacggct cggtccttcc ggccggcaag tacgacgtca agatcgacta caagggcttc
      421 ggaaactccg ccgagttcct gttcctccag ggcggcgccc tgaagggcaa gtgccccgcc
      481 gaggcgcgcg gcttcccgtc ccaggccccc gcgggcgccg cggacgcgaa cgccgtgaag
      541 ctgcacgacg tgaagatcac cgacccggcc gcccagaagg gctacacggt caagacgaat
      601 ccgggcacgg aaaaggtgtt cccgaaggtg gagacgggcg acaaggtcca gcccggcgcc
      661 gccgcggcat ccttctcctg gggcgcgcac gggttcgccc cgggcatcgt gggcaagacg
      721 gcaccggccg gcaggagcct caagctctcg ttcgactcgg ccaacagcgc cgccggattc
      781 agcgcgcttc tcccggccgt tcagaaatag ctcgcgcagc acgggggaac cgggccccgg
      841 ttcccccgga ccccctccgg gctacttgct ctgctgctgc ctcttcatca tctcttcgat
      901 ctgcttgcgc tgctcgggcg tgaggccctc catcgccttg cgcatctcgt cgttcgcgcc
      961 gggaggggcc atgacgctcg cggcgcccat catcccttcc ttcttggcgt agccggccgg
     1021 gatctcgaag agcgccgcct tgagcgcctt ccgctcgacc ttcgtcagct ccatcgtggc
     1081 gagcggcgcc gggttgcctt tctcgcgcgt gatcatcttc acgacgaacc cgtccacgcc
     1141 cgcgtcgcgc agggctttca tcatgccctc gtcgctctgg ttcccacggc gcatgagccc
     1201 gcgcatggac tcgtagctca ggcccgagat gtccttcgag gtccagagtt cctgctcgga
     1261 cttggcgtcc tcggcccgcg tgacgagaac gtgctcgctg gaatacccgt tgaccgtttc
     1321 cttcccgagc ttcttgacgg accacgtctc tttcgacttg tcccggtact tcgcggtctg
     1381 ctccctcatc ttcttgaggt cgatctccgc gtaggacttc tgctcgtcgt tgatcatgta
     1441 catgatctcg ggattcgacg ccttcatgag cgtcgtcatc ttgaacggca tctgggccgt
     1501 ctggatgtcg agttccgacc gggccccggc gctcgagacg taggccttgc ccgtcccgtt
     1561 tccttccctc atcgtgagct tcatcgtgag ctcgccctcg aactgggcga gggcgggtcc
     1621 ggcggccagg acggctccgg ccagcatcgc aagaacggga ttcttcatgc gctctccttc
     1681 cgtgtgaggc ccggcatcgg gcgcgagaag tctagcgcag cgcagggctt cagctcgccc
     1741 ggagcgcgac gacctggcgg tcctcgaacg acacgacgac gagcgtctgg tcgtcctcgg
     1801 ggggccggtt gcgcgtgaag ctcttgaccg actcgaggag cgcctgcttg aggacgtccg
     1861 cgcccttcgt cgcgcgcgag aggatcacgc cctcgaaccg ctcgaagccg aacggctcgc
     1921 cgtcgtcgtt cagcgcctcg atgagaccgt cgctgtagag gacgaggcgg tccccgggct
     1981 cgaaccgcac gtcgaactcg cggaacggct cgcgccgcct cacgcccagc gggaagcccc
     2041 aggacgagag catctcgagc ttcttctcgc gcgcgcggaa cacgtacggg tcgaggtggc
     2101 ccgcggacgc gaaccgcagc gtcgaggtct cggggtcgag gacgccgtag aagaacgtca
     2161 tgagcatgcg cttcggcgcc gtcttcatga cgatgtcgtt caggacctcg aggacgcgga
     2221 cgggcgacac gtcgtgccgc acctggacga ggagcgccgc cttcgtcatc gccatcacga
     2281 tgccgctcgt gaggccgtgc ccggccacgt ctccaaacgc gaggccgagc ttcccgtccg
     2341 gaagcggaag gaagtcgaag taatcgccgc cgatttcggt cgcggcgcgg taggaggacg
     2401 cgagcgaagc cgacccgagc tccgggtgcg aagacggcag gagcgccgcc tggatgcggt
     2461 ttgccgcgtc aatctcggcc ttgatgcgct cgcgctcgac gatgcgcttc acgtggggcg
     2521 cgaggtcctc gtacgcgtac acgatcgtgc gcccggacgc gaggccgagg gccgacacgc
     2581 cgaggaggag cccgagcggg agcgcgaggc ccatcagcgg ccccgcgagc gccgggcccg
     2641 acgcgacggc cgcgagcggg aggaagctca ggaggaggcc gcccccgaac gccgccgtcg
     2701 cggcggcgag gagatcggag aacgcgaaga cgaggaggac gacggcgatc ccgccgaagc
     2761 ccaccatgag gctccgctcg aacggcccga cgggcggcgg gaggacgccc atgagcgtcc
     2821 cgaagagcgc cgcgagcacg gcgcccacag cgaggaggcg cctcctgtgg aagaaggcga
     2881 ggaagaagag gaggccgacg ctcgcgaaga agagagcgtc gagcgccgcc cccgcgttca
     2941 ccgcgagggg cccccagatc gcgttcaacc cgtcgcgcgt gccgggcgcg aggcgcgccc
     3001 agacggc
//
LOCUS       DAOMFY010000379         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_14154_length_2990_cov_9.606814, whole genome shotgun sequence.
ACCESSION   DAOMFY010000379 DAOMFY010000000
VERSION     DAOMFY010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14154_length_2990_cov_9.606814"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLB01_18880"
     CDS             <1..213
                     /locus_tag="PLB01_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79420.1"
                     /translation="RATAREPADRYPGVPALAGDVARFLDGERVEAYAEGPAERALRI
                     LKRHRVAVGLVAVYLLTRGLFILLRR"
     gene            256..606
                     /locus_tag="PLB01_18885"
     CDS             256..606
                     /locus_tag="PLB01_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79421.1"
                     /translation="MNKVLLGLVVGAALGAVDGLTAWFTPEVRSEIAGIVAGSTFKGL
                     VAGVLIGVFARKVQSVPATLVFGTALGLVFAFLVANMQGKYYFEIMLPGAIVGLLTGY
                     ATQRYGRKTEASAA"
     gene            complement(619..2049)
                     /locus_tag="PLB01_18890"
     CDS             complement(619..2049)
                     /locus_tag="PLB01_18890"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQQ79422.1"
                     /translation="MRSPSRFLPIAAGLHVLMLAAAAPAAGSSVTDKGGDFDVLKKRR
                     VIRVLVVSNKTNYFIERGVPRGLTYEVFRMFEDELNRKYKTGNLKINVVFRPVGRGDL
                     AAALLDGRGDVAAAGLTVTPERLAQFDFSTPTVKNVSEIVVSGPASEPVASAADLSGR
                     EVFVRKGSSVHESLDALNADLSKSGKPPVKLRFAPDHLEDEDLLEMTNAGLVSYVVVN
                     DHLARFWASVLPALRVHPEAVLRSGAGIAWAMRKNSPLLKAELDAFLKKNPEGSATYN
                     MLFQKYLKSTKFVKNSASKEEMRKFQEMVTFFKTYSDKYDMDFLLMAAQGYQESQLNH
                     AAKSPVGAVGVMQVMPATGKELKVGDITQLGPNIEAGVKYMRFMIDQYFEKEPMDRLN
                     KGLFAFAAYNAGPNRIQTLRKETAKRGLDPNQWFNNVEIVVSEKIGRETVTYVSNIYK
                     YYVAYKLLTESQAERKKDREEAQGVK"
     gene            2431..2817
                     /locus_tag="PLB01_18895"
     CDS             2431..2817
                     /locus_tag="PLB01_18895"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ79423.1"
                     /translation="MARKRKTKPGKTETLGDRIRRYRIAKGLTQAELAAQIGVPQQTI
                     TYYEVRGVSPPPALLVAIADALDVSTDILLGRKKALGDAGAAPTGSVQRQKHLKRLDA
                     LPPHDQKAVFKIIDALAERRAKSRGA"
BASE COUNT          480 a          962 c         1016 g          532 t
ORIGIN      
        1 cgcgcgacgg cgcgggagcc cgccgaccgc taccccggcg tcccggcgct cgccggcgac
       61 gtcgcgaggt tcctggacgg tgagcgcgtc gaggcctacg ccgaggggcc tgccgagcgg
      121 gcgctccgga tcctgaagcg tcaccgggtc gcggtcgggc tcgtcgcggt ctatctcctc
      181 acgcgcgggc tcttcattct tctccgccgc taaaggaatt cccgcccgcg ttgaatagag
      241 agtcaggagg atccgatgaa caaagtcctg ctcgggttgg tggtcggggc ggcgctcggg
      301 gccgtcgacg gactcacggc atggtttacc ccggaggtga ggtcggagat cgcggggatc
      361 gtggcggggt cgacgttcaa ggggctcgtg gcgggagtcc tgatcggcgt cttcgcccgg
      421 aaggtccagt ccgtgcctgc gacgctcgtc ttcgggacgg cgctcgggct cgtcttcgcg
      481 ttcctcgtcg cgaacatgca gggcaagtac tacttcgaga tcatgctgcc gggcgcgatc
      541 gtcggcctgc tgaccggcta cgcgacgcag cgttacgggc ggaagacgga ggcctcagcg
      601 gcttagggga acgcgcggtc acttcacgcc ctgcgcctct tcgcggtcct tcttgcgctc
      661 cgcctgcgac tccgtgagca gcttgtaggc gacgtagtac ttgtagatgt tcgacacgta
      721 cgtcacggtc tcccggccga tcttctcgga caccacgatc tcgacgttgt tgaaccactg
      781 gttcgggtcg agcccgcgct tcgcggtctc cttgcgcagc gtctggatcc ggttcgggcc
      841 cgcgttgtag gccgcgaacg cgaagaggcc cttgttcagg cggtccatgg gctccttctc
      901 gaagtactgg tcgatcatga accgcatgta cttcacgccg gcctcgatgt tcgggccgag
      961 ctgggtgatg tcgccgacct tcagctcctt gccggtcgcg ggcatgacct gcatgacgcc
     1021 gacggcgccc accgggctct tcgcggcgtg gttcagctgc gactcctggt agccctgcgc
     1081 cgccatcagg aggaagtcca tgtcgtactt gtccgagtac gtcttgaaga acgtgaccat
     1141 ctcctgaaac ttccgcatct cttccttcga ggcggagttc ttcacgaact tcgtgctctt
     1201 caggtacttc tggaagagca tgttgtaggt cgcggaccct tccgggttct tcttcaggaa
     1261 cgcgtccagc tcggctttca ggagcgggct gtttttccgc atggcccagg cgatccccgc
     1321 gcccgagcgg agtacggctt ccgggtggac ccggagggcg ggcagcacgc tggcccagaa
     1381 gcgggcgagg tgatcgttca cgacgacgta cgaaacgagc cccgcgttcg tcatctcgag
     1441 caggtcctcg tcttcgaggt gatcgggcgc gaagcgcagc ttgaccgggg gcttgccgct
     1501 cttcgaaaga tcggcgttca gcgcgtcgag gctctcgtgg acgctcgagc ccttccgcac
     1561 gaagacctcc cgcccggaaa ggtcggccgc ggaggcgacc ggctcggacg ccgggcccga
     1621 gacgacgatc tcggagacgt tcttcacggt cggggtcgag aagtcgaact gcgcgaggcg
     1681 ctcgggagtg accgtcaggc cggccgccgc gacgtcgccg cgcccgtcga gaagggcggc
     1741 cgcaaggtcg ccgcggccga ccgggcggaa gaccacgttg atcttcaggt tgcccgtctt
     1801 gtacttccgg ttcagctcgt cctcgaacat ccggaagact tcgtacgtga ggccccgcgg
     1861 cacgccccgt tcgatgaagt agttcgtctt gttggagacg acgaggaccc ggatgacccg
     1921 gcgcttcttc aggacgtcga agtcgcctcc cttgtcggtg acggaagacc ccgccgcggg
     1981 cgcggcggcg gcgagcatca ggacgtgaag gcccgcggcg atcggcagga atcgcgaagg
     2041 agaacgcatg gctgatcctc ctgagacccg tctatcttct acccggaagg gccggagggg
     2101 ggcgaggtca cccgcgccga cgaaatgctc aggcgggccg tttccgttca gctggaggaa
     2161 cactcgaccg ctgtgcgcag gagtgagatt tgaggcggaa tgagatggtg gagctgaacg
     2221 ggatcgaacc gtcgacctcc tgaatgccat aaagggagac gggctgatta tctgcgaaaa
     2281 cgtgaggtat tcccggagcc gagtgtcgat gtcgagggaa ttgacccctg accattccct
     2341 gtaccgccga gggcgaatta cagaagactt cggtaagcga ccggtgtcga cagcaagtgg
     2401 ttgccgcatc cgctcctaga cttgagcgct atggcgcgga agcggaagac gaagccgggt
     2461 aagacggaga ctctcggaga ccggattcgt cgctatcgga tcgcgaaggg gctgacacag
     2521 gccgagctcg cggcgcagat cggcgtgccg cagcagacga tcacgtacta cgaggtgcgc
     2581 ggtgtgtcgc cgccgccggc gctgctcgtc gcgatcgcgg acgcgctcga cgtctcgacc
     2641 gacatcctgc tcggcaggaa gaaagcactg ggcgacgctg gcgccgcgcc gacgggcagc
     2701 gtccagcgac agaagcacct taagaggctc gacgcgttgc ctccgcacga ccagaaggcc
     2761 gtcttcaaga tcatcgacgc actggcggag cgacgggcga aaagtcgcgg cgcctgaggc
     2821 ccgcgacgtc actccgcttg ctctcgctcg cgcgcgacca cctgcgcgcg ccattccgcc
     2881 tgctcggacg tcgcgcgctc gcggcaagcg tcgcgccgcg ctcccggctc tcgacttcgg
     2941 actcctgatc ggcttccgtc ccggcgcacc cgacgtacca gccgcccccg
//
LOCUS       DAOMFY010000380         2979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_14252_length_2979_cov_6.941860, whole genome shotgun sequence.
ACCESSION   DAOMFY010000380 DAOMFY010000000
VERSION     DAOMFY010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14252_length_2979_cov_6.941860"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..955)
                     /locus_tag="PLB01_18900"
     CDS             complement(176..955)
                     /locus_tag="PLB01_18900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQQ79424.1"
                     /translation="MVGALAALLGAGGIGLSLRFNWWRPRRPGTPILMYHEVGPHRAG
                     SKLNHWRVLPEDFARHLDHLARRGFRGVALCNLLDRPSADGRRAVITFDDGYEGVLSR
                     ALPLLAAHGFSATVFVVSGRLGGVNDWDGERPGDALLSADGVRALHAAGIEIGSHGAT
                     HRALPGLSEADLAAEVAGSKAALEAVTGAPVVSFCYPYGAFDDRSVEAVRAAGYRAAT
                     VIRGGIPPDLSDPFRLKRVAVRGTNTLLDFSLALTRGRSRL"
     gene            complement(955..1815)
                     /locus_tag="PLB01_18905"
     CDS             complement(955..1815)
                     /locus_tag="PLB01_18905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ79425.1"
                     /translation="MSDPAGPPLTVTLIARDEEDRLPEALAAVAFADEIVVVVDSATT
                     DRTAEIAEAAGARVLVRAFDGFGTQKNAAAALATNRWVLSIDADEVVSPALAHEIRSR
                     LAATASEMNPPAAFRIPIRLEFLGRELHFGRDTVVRPARLYDRTRARFSEDPVHEKVV
                     ASGPVESLDESILHRSYRDLAHYLEKLDRYTTLAAEAKWAARKGAMPFLPVRVAWEFF
                     DRAILRLGILDGTAGLTFAALSSANTLLKYLKLRELEDAIARGERLPPTNARAEDTAV
                     LRIRALMRFR"
     gene            1853..>2979
                     /locus_tag="PLB01_18910"
     CDS             1853..>2979
                     /locus_tag="PLB01_18910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ79426.1"
                     /translation="MSAGRPSEDPEASGRLLFAAALAAGALLRLILLGRPDLFAADEG
                     RWAVGARNVLEGGAQVLAPSTTPLGDPAGTPILFPWLLSGMVRVFGAEEWAVRFPSAI
                     LGLFAAFVVERIVRRGWGQPAGHLAGAFVALFPPLVAASRVATVETALVTLGLAGIIF
                     GLRAFEEDIPWEGALSGAFFGLGFLAKGYAIGLFLLPLLVALAARPRLFALGRTKRSL
                     LLLFAAFVAVGGSHLLAVLFLRPETFAFQLATAFGASEAAARTAALPTAFSADLLSIL
                     RPVFWFLPVTLLGVAFLGRDVGEDEIASGATGGDRRLAHGLLWGAYFVELVIVVAVAG
                     RYRLSSIPVLPAVATLTGLGATAVVVSARARASRTTQRVAAG"
BASE COUNT          348 a         1056 c         1141 g          434 t
ORIGIN      
        1 tcgcgcgcaa gtcggcgcgc acgtggccgc tcgccgagcg cgtcgcgccc gcggcggcgg
       61 cgctcatcga ctgggacccg ggcgacctcc cgatcgtgcc cgtgctgctg gaccttcccg
      121 acaccgcctc gcgcgacgcg ctccgccagc gcgccatctc ctggggcgag acggtctaga
      181 gcctcgaccg cccccgcgtc agggcgagcg aaaaatccag cagcgtgttc gtgccgcgga
      241 cggcgacacg cttgaggcgg aacgggtcgg aaaggtcggg cgggatgccg ccgcggatga
      301 cggtcgcggc gcggtagccc gcggcgcgca cggcctcgac gctgcggtcg tcgaacgcgc
      361 cgtacggata gcagaaggag acgacgggcg cgccggtcac ggcctcgagc gccgccttgc
      421 tgcccgcgac ctcggccgcg aggtcggcct cggagagccc ggggagcgcg cggtgcgtgg
      481 cgccgtgcga gccgatctcg atcccggccg cgtgaagcgc ccgcacgccg tcggccgaga
      541 ggagcgcgtc gcccggcctc tcgccgtccc agtcgttgac gccgccgagc cttcccgaga
      601 cgacgaaaac ggtggccgag aagccgtgcg cggcgagaag ggggagggcg cgcgagagca
      661 cgccctcgta cccgtcgtcg aacgtgatca ccgcgcgccg gccgtccgcc gacggccggt
      721 cgaggaggtt gcacagggcg acgccgcgga acccgcgccg cgcgaggtgg tcgaggtgcc
      781 gcgcgaagtc ctccggcagg acgcgccagt ggttgagctt cgagcccgcg cggtgcggcc
      841 cgacctcgtg gtacatgagg atgggcgtgc cggggcgcct cggtcgccac cagttgaatc
      901 gaagcgaaag gccgatcccg ccggccccga ggagtgcggc gagggcgccg accatcagcg
      961 gaaccgcatg agcgcgcgga tgcggaggac ggcggtgtcc tcggcgcgcg cgttcgtggg
     1021 gggcaggcgc tcgccgcgcg cgatggcgtc ttcgagctcg cgcagcttga ggtacttgag
     1081 aagcgtgttc gcggacgaga gggccgcgaa cgtgaggccc gcggttccgt cgaggatgcc
     1141 gagcctgagg atggcgcggt cgaagaactc ccaggcgaca cggacgggga ggaagggcat
     1201 ggcgcccttc cgggcggccc acttcgcctc ggctgcgagc gtcgtgtagc ggtcgagctt
     1261 ctcgaggtag tgcgcgaggt cgcggtacga gcggtgaagg atggactcgt cgagggactc
     1321 gaccgggccg ctcgcgacga ccttctcgtg gacggggtcc tccgaaaagc gtgcacgggt
     1381 gcggtcgtag agccgcgcgg gccgtacgac cgtgtcgcgc ccgaagtgga gctcgcgccc
     1441 gaggaactcg agccggatcg ggatccggaa cgcggccggc gggttcatct ccgacgccgt
     1501 ggccgcgagg cgggagcgga tctcgtgcgc gagggccggc gagacgacct cgtcggcgtc
     1561 gatcgagagg acccagcggt tcgtcgcgag cgcagcggcg gcgttcttct gcgtcccgaa
     1621 gccgtcgaac gcccgcacga ggacgcgcgc tcccgccgct tcggcgatct cggcggtgcg
     1681 gtccgtcgtc gcggagtcca cgacgacgac gatctcgtcg gcgaatgcga cggccgcgag
     1741 cgcctcggga aggcggtcct cctcgtcgcg cgcgatgagc gtgaccgtga gaggcgggcc
     1801 ggccgggtcc gacatcgggg gaagagtatg aaccgtccgt atactccgca tcgtgtccgc
     1861 cgggcgtccg tccgaagatc ccgaagcgtc cggccgtctc ctctttgcgg cggcgctggc
     1921 ggccggagcg ctgctccggc tgattcttct cgggcggccg gatctcttcg ccgccgacga
     1981 gggccgctgg gcggtcggcg cgcgcaacgt cctcgaaggc ggcgcccagg tgctcgcgcc
     2041 gtccacgacg ccgctcgggg acccggccgg cacgccgatc ctctttccgt ggctgctctc
     2101 cggcatggta cgcgtcttcg gcgccgagga gtgggccgtg aggttcccgt cggcgatcct
     2161 cggcctcttc gcggccttcg tcgtggagcg gatcgtgcgc cgcggctggg ggcagccggc
     2221 gggccacctc gccggcgcgt tcgtcgcgct gttcccgcct ctcgtcgcgg cctcgcgcgt
     2281 cgcaaccgtc gagacggcgc tcgtgacgct gggtctggcg gggatcatct tcggcctgcg
     2341 cgcgttcgag gaggacatcc cgtgggaggg cgcgctgtcc ggcgccttct tcggcctcgg
     2401 cttcctcgcg aagggctacg cgatcgggct cttcctcctg ccgctcctcg tcgcgctggc
     2461 cgcgcgcccg cgcctcttcg cgctcgggcg gacgaagagg tcgctgctcc tcctcttcgc
     2521 ggcgttcgtc gcggtcggcg ggtcgcacct gctcgccgtg cttttcctcc gccccgagac
     2581 gttcgcgttc cagctcgcga ccgccttcgg ggcgtccgag gccgcagcgc ggaccgccgc
     2641 gctcccgacg gcgttctccg cggacctcct gtcgatcctg aggcccgtct tctggttcct
     2701 gcccgtcacg ctcctcggcg tcgccttcct cgggcgcgac gtcggcgagg acgagatcgc
     2761 gtcgggcgcg acgggcggag accggcggct cgcgcacggc ctcctctggg gcgcgtactt
     2821 cgtcgagctc gtgatcgtcg tcgcggtggc gggacgctac cgcctctcgt cgattcccgt
     2881 cctgccggcc gtggcgacgc tcacgggcct cggggcgacg gcggtcgtcg tctcggcgcg
     2941 tgcgcgcgcc tcgcggacga cgcagcgggt ggcggcggg
//
LOCUS       DAOMFY010000381         2978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_14263_length_2978_cov_7.477592, whole genome shotgun sequence.
ACCESSION   DAOMFY010000381 DAOMFY010000000
VERSION     DAOMFY010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14263_length_2978_cov_7.477592"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="tagF"
                     /locus_tag="PLB01_18915"
     CDS             complement(<1..910)
                     /gene="tagF"
                     /locus_tag="PLB01_18915"
                     /inference="COORDINATES: protein motif:HMM:NF021393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HQQ79427.1"
                     /translation="MGFLSRFQKPSADDAPPIESWPLFAFGKLPVYKDFISAGLTDDV
                     SREFRDWLSDGFSKVWSARDDCRSAEIPLHAFLLRLPASRKMAAGVLWGSTDQGGLRK
                     FPFSLFTILPAGRPAAAVLTALDYLPVFESRGREIRRKYDAGGSLADVYQELRGARID
                     VPLRTPEQIRLKLAEALASRRVGPLARALFESDAETRWSALLSGLDAAARHPAAGAAA
                     FRVPLDEDTSPLDQLKLWTVRLAKASPGGASPAGVLYRTGGAAPRGVVFFRDARAEDI
                     LLLHPEAIPTDFVEEIPPPVASRPPAE"
     gene            complement(930..>2978)
                     /locus_tag="PLB01_18920"
     CDS             complement(930..>2978)
                     /locus_tag="PLB01_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79428.1"
                     /translation="GGDALPPPLPALPPPSFPGTTLAPTFHPAAQTRVNTSRLQELIG
                     RLQVDSANLGAQKSGFAFLLDESTTPGKTKLANPTVLVSGLLADVGAYADVDAFRASD
                     DGKILAQVNTISSLDGKKQKLEEWQKRQEDLKNASVAKAPDFERTMPDPRFRASDVVP
                     FARRTAELALLARVLPPVQEFLTASIGPGCNPAICFQKPFTQMMIPYGNGVVALAERQ
                     TNALAREDAKSDVKAVSQGEIEYLQRFLDATGAPVRSGGGGFSIPASALTARSWRAYQ
                     IAVGTWTPAGGGGGGGAPDQAGQLTLADLQGFVQTNANLNPVIGYYKSRFEARASAKP
                     AAVPDYLLAAAETFKRTVGALGDKDELKSWKQLATGAEGATLKGWHAFTGDLRIKSGP
                     YGRSLKSLEDRGGNLIKAAAEPQFNPRWDALYQRLASCCSGQFPFITESRMKQERADY
                     AAERATGGLSLRRNLPTIGQADVLGVLTEVGVLSDEYAVDPLLGGNEPSFDFIQQNRG
                     ALAVARLWQRFISGAGSTAGASLAGKPGGPGGREVSVLAIDREPGAGHRPLKDRVSQI
                     TLFDRGVILRPSTDIKSQRIPPPFVWRLSSADALLTISGRNEQSGEGGWTSTYEVTGG
                     PLKLFYFLRLASESRTELSDPKVWTIRVQIPDGRNSREILEGAFEIRLDEPMPPILPN
                     "
BASE COUNT          427 a          991 c         1092 g          468 t
ORIGIN      
        1 cttctgctgg cggtctggac gcgacgggcg gcgggatctc ctcgacgaag tcggtcggga
       61 tcgcctccgg gtggaggagg aggatgtcct cggctctcgc gtcgcggaag aagacgacgc
      121 cgcgtggagc cgcgccgccg gtccggtaga gaacgcccgc cggggacgct cctcccggcg
      181 aggccttcgc gagccgcacg gtccagagct tcagctggtc gagtggagac gtgtcctcgt
      241 cgagcggaac gcggaaagcc gcggctcccg ccgcaggatg ccgggcggcc gcgtcgagac
      301 cggaaaggag ggcggaccat cgggtctcgg catcgctctc gaacagggct ctcgccagtg
      361 gcccgactcg ccggctcgcg agcgcctcgg cgagcttcag ccggatctgc tcgggcgtcc
      421 ggaggggaac gtcgatccgg gccccgcgga gctcctggta gacgtccgcg agggagcccc
      481 cggcgtcgta cttccgcctg atttcgcggc cgcggctctc gaagaccgga aggtagtcga
      541 gcgccgtcag gacggccgcg gccggcctcc cggccgggag gatcgtgaag agggagaacg
      601 gaaacttccg gaggcctccc tggtccgtgc tgccccagag aacgccggcg gccatcttgc
      661 ggctcgcggg caggcgcagc aggaatgcgt ggagcgggat ttcggccgaa cggcagtcgt
      721 cgcgcgcgga ccacaccttg ctgaatccgt cggacagcca gtcgcggaac tcccgcgaga
      781 catcgtcggt gagccccgcg gagatgaagt ccttgtagac gggcagcttc ccgaacgcga
      841 agaggggcca cgactcgatc ggaggcgcgt cgtccgccga tggtttttgg aatcgggaca
      901 ggaagcccac gggactctct cgacggcctt cagttcggaa ggatgggcgg catcggctcg
      961 tcgaggcgga tctcgaacgc tccctcgagg atctcccgcg agttccttcc gtccgggatc
     1021 tggacacgaa tggtccagac cttcggatcc gagagttcgg tccgcgactc gctggcgaga
     1081 cggaggaagt agaacagctt caagggcccg ccggtcacct cgtacgtgct cgtccatccc
     1141 ccttcgccgg actgctcgtt ccggccgctg atcgtgagga gggcgtcggc cgagctgagc
     1201 cgccagacga acggaggcgg gatcctctgg gacttgatgt ccgtcgaggg ccgcaggatc
     1261 acgcccctgt cgaagagcgt gatctgcgag acgcggtcct tcagcgggcg gtgtcccgcg
     1321 ccgggctcgc ggtcgatggc gaggacgctg acctcgcgcc cgccggggcc gccgggcttc
     1381 ccggcgagcg acgcgcccgc cgtggagccg gcgccgctga tgaaccgttg ccagaggcgg
     1441 gcgaccgcga gagccccgcg gttctgctga atgaagtcga aggacggctc gtttccgccg
     1501 aggagcgggt cgacggcgta ctcatccgaa aggacgccga cctcggtcag gacgccgagc
     1561 acgtcggcct gtccgatcgt cgggaggttc cgccggagtg agagtccgcc cgtggcgcgc
     1621 tcggccgcgt agtcggcgcg ttcctgcttc atgcggctct cggtgatgaa ggggaactgt
     1681 ccggagcagc aggacgcgag cctctgatag agcgcgtccc acctggggtt gaactgcggc
     1741 tcggcggcgg ccttgatgag gttgccgccg cggtcctcga ggctcttcag agatcgtccg
     1801 tacggcccgg acttgatcct caggtcgccg gtgaacgcgt gccagccttt cagcgtcgct
     1861 ccttcggctc ccgtcgcgag ctgcttccac gacttcagct cgtccttgtc gccgagagcg
     1921 ccgacggtcc gcttgaaggt ctcggcggcg gcgaggaggt agtcggggac cgcggccggc
     1981 ttggccgagg cgcgggcctc gaatcggctc ttgtagtacc cgatgacggg gttgagattc
     2041 gcgttcgtct gcacgaaacc ctgcaggtcg gcgagcgtca gctggccggc ctggtcgggc
     2101 gcgccgccgc ctcctccgcc gccggcggga gtccatgtcc cgacggcgat ctggtacgcc
     2161 cgccagctgc gggccgtcag ggcggaggcg ggaatcgaga acccgccgcc tccggaccgc
     2221 accggggcgc ccgtggcgtc gaggaagcgc tgcaggtact cgatctcgcc ctgcgagacg
     2281 gccttgacgt cgctcttcgc gtcctcgcgc gcgagcgcgt tcgtctgccg ctccgcgaga
     2341 gcgacgacgc cgttgccgta gggaatcatc atctgcgtga acggcttctg gaagcagatc
     2401 gcggggttgc agcccgggcc gatcgacgcg gtcaggaatt cctgcaccgg gggcagcacg
     2461 cgcgcgagaa gcgcgagctc ggcggtgcgc cgcgcgaagg ggacgacgtc gctcgcccgg
     2521 aaccgcggat ccggcatcgt ccgctcgaag tccggcgcct tcgcgacgga cgcgttcttc
     2581 aggtcttcct gccgcttctg ccactcctcg agcttctgct tcttgccgtc cagggacgag
     2641 atcgtgttca cctgggcgag gatcttcccg tcgtcgctcg cccggaacgc gtcgacgtcc
     2701 gcgtacgcac cgacgtccgc gaggagaccg ctgacgagga cggtcgggtt cgcgagcttc
     2761 gtctttccgg gcgtcgtcga ctcgtcgagc aggaacgcga agccgctctt ctgggcgccg
     2821 agattcgcgg agtcgacctg aaggcgcccg atcagctcct ggagcctcga cgtgttgacg
     2881 cgcgtctgcg ccgccgggtg gaacgtcggt gcgagcgtgg tgcccggaaa cgacggcggc
     2941 gggagcgccg gaaggggcgg cggcaacgcg tcgccgcc
//
LOCUS       DAOMFY010000382         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_14489_length_2951_cov_7.391229, whole genome shotgun sequence.
ACCESSION   DAOMFY010000382 DAOMFY010000000
VERSION     DAOMFY010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14489_length_2951_cov_7.391229"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLB01_18925"
     CDS             complement(<1..1033)
                     /locus_tag="PLB01_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HQQ79429.1"
                     /translation="MIPRPPFSSDLTLERELAEFEALKPRLLAVWDLIAGRDEEPHTS
                     VVVPSLTLDQAELTKLDGASFYEERLLFLLIRLRNPRARVVFVTSQPVHPLVLEYYFQ
                     LLVGIPASHARGRLTLLCAHDASPRSLTEKILERPRLIERIRAGIPDRSRAYLTVFNS
                     TPLERRLAVLLGIPLNGVDPALSDYGTKSGSRKVFREAGVACPEGHEDLRDDVDLEAS
                     LVDLKRRNPALRKAVVKLNDSFSGEGNAIFRYPDGPTDAAAVRERLPKLEFSVGWETW
                     DDFHAKFRRMGGIAEEFLEGAIKTSPSVQLRSGPRGDVLPISTHDQVLGGPSGQVFLG
                     CLFPAADGYR"
     gene            1117..1614
                     /locus_tag="PLB01_18930"
     CDS             1117..1614
                     /locus_tag="PLB01_18930"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HQQ79430.1"
                     /translation="MRTRRAGGRGFTLIELLIVVAIIGIIVAISVVNMINAIQRGKQK
                     RSMADIKSVSTALEAYMTDFNSYPAAAGYSLPTGLSLPTATLGTASGVLSPTYIRVAP
                     LSDGWNSWYLYGSSALRADYVVRSAGADGAPQASPLYGPTTDFNADIILVDGAFVQFP
                     EGAQR"
     gene            1784..2284
                     /locus_tag="PLB01_18935"
     CDS             1784..2284
                     /locus_tag="PLB01_18935"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQQ79431.1"
                     /translation="MKRNQKGFTLIELLIVVAIIGIIVAIAIPNLLNAIQRAKQKRTM
                     GDMRSAGTAAEAYAVDFNHYPAAAGYSLPSGLSFGSTTFNTGGGASSFSGQVTPTYIR
                     VLPMADGWQSYFFYASTGQLYGILSAGKDGAKDSTITGGETTDFNNDIIFVNGQFVQY
                     PAGAQR"
     gene            2370..>2951
                     /locus_tag="PLB01_18940"
     CDS             2370..>2951
                     /locus_tag="PLB01_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79432.1"
                     /translation="MKRGKATGRTALLGGALGLAFAAALLGPGALAGLVPARGDLADF
                     FWPMKAYTAARWAAGSLPLWNPLSGCGEPWLAQLQTGVLYPGDLPFLLGGAAGPLLAI
                     ALHLSIAAAGMAAWLSDLGTSRAGALAGAAIFAGGGAFLSLAPVYNNFCTAAFLPWLF
                     LGARRAVRGASPAPFALAVALAFLGGEPALAAVG"
BASE COUNT          441 a          996 c         1031 g          483 t
ORIGIN      
        1 gccggtagcc gtcggcggcg gggaagaggc agccgaggaa gacctggccg ctcggcccgc
       61 cgaggacctg gtcgtgcgtc gagatcggaa ggacgtcgcc gcgcgggccg ctgcggagct
      121 ggacggaggg cgacgtcttg atcgcccctt cgaggaactc ctcggcgatg ccgcccatgc
      181 gccggaattt cgcgtggaag tcgtcccagg tttcccaccc gacggagaac tcgagcttcg
      241 gaagtcgttc gcggacggcg gcggcgtccg tcggcccgtc ggggtagcgg aagatcgcat
      301 tgccttcgcc cgagaagctg tcgttcagct tcacgaccgc cttgcggagc gcggggttgc
      361 gtctcttgag atccacgagg gaggcctcga gatccacgtc gtcacggagg tcctcgtggc
      421 cctccgggca cgcgacgccc gcctcgcgga agaccttccg gctcccggac ttcgtgccgt
      481 agtcgctgag cgccgggtcg acgccgttca gcgggatgcc gaggaggacg gcgagccgcc
      541 gctcgagcgg ggtcgagttg aagaccgtga ggtaggccct cgaacggtcg gggatgcccg
      601 cgcgaatgcg ttcgatgagc ctcggacgct cgaggatctt ctcggtgagc gaccggggag
      661 aagcatcgtg cgcgcagagg agcgtcagcc ggccccgggc gtggctcgcc gggatgccga
      721 cgaggagctg gaagtagtac tccaggacga gggggtggac cggctgggac gtgacgaaga
      781 cgacccgggc gcgggggttg cgcaggcgga tcaggaggaa aaggaggcgc tcctcgtaaa
      841 agctggcccc gtccagcttc gtcagttcgg cctggtccag ggtgagggac ggcacgacga
      901 cggacgtgtg gggctcctcg tcacggcctg cgatcaagtc ccagaccgcg aggaggcgcg
      961 gcttcagcgc ctcgaactcg gcgagctcgc gctccagggt caggtcggac gaaaacgggg
     1021 gcctcggaat cacgtgcggg gattctaggg cccgggccgg gagcgaggca cggatcttgt
     1081 ttacggccgg gggagaccga gaggaaagag gcgccattga ggacacggcg ggccggcggg
     1141 cggggcttca cgctgatcga gctcctgatc gtcgtcgcga tcatcgggat catcgtcgcg
     1201 atctccgtcg tcaacatgat caacgcgatc cagcgcggga agcagaagcg ctcgatggcg
     1261 gacatcaaga gcgtctcgac ggcgctcgag gcctacatga cggacttcaa ctcctatccg
     1321 gccgctgcgg gctattcgct tccgacgggc ctttccctgc ccacggccac gctcggcacg
     1381 gcctccggcg tcctcagccc cacgtacatc cgggtggcgc cgctctcgga cggctggaac
     1441 tcgtggtacc tctacggctc gagcgcgctg cgcgcggatt acgtggtccg ttccgccggc
     1501 gccgacggcg ctccgcaggc ttcgcccctc tacggcccga cgacggattt caacgcagac
     1561 atcatcctgg tggacggcgc cttcgtgcag tttccggagg gcgcccagcg ctgaagatcg
     1621 ggctcgccgg gtgacaatcg ctggaaggtt gccttcgtcc tccccgacaa taattcgtca
     1681 cgatgcggcc gatccgggcc tcggcctgct tcaattgttg aaaagagaag agatcggcga
     1741 acggcacggc tcttgaatgg aagcggacgg aggttgatcc caaatgaaaa ggaaccagaa
     1801 gggtttcacg cttatcgagc ttctgatcgt cgtcgcaatc atcggcatca tcgtcgcgat
     1861 cgccattccc aacctgctca acgccatcca gcgcgccaag cagaagagga cgatgggtga
     1921 catgcgctcg gccggcacgg ccgccgaggc gtacgccgtc gacttcaacc actacccggc
     1981 ggccgctggc tactcgctcc cgagcggact ttcgttcggg tcgacgacgt tcaacacggg
     2041 cggcggcgcg tcgagcttca gcgggcaggt cacgcccacg tacatccgcg ttctcccgat
     2101 ggcggacggc tggcagtcgt acttcttcta cgcctcgacc ggccagctct acggcatcct
     2161 gtccgccggc aaggacggcg cgaaggactc gaccatcacc ggtggcgaga cgaccgactt
     2221 caacaacgac atcatcttcg tgaacggtca gttcgttcag taccccgcgg gcgctcagcg
     2281 gtaagtcctc ccgccgtcgc ccggaagagc ccccgcgagg gggctcttcc ttttttgggg
     2341 cgatcctctg cgcgcctaga atcctcgcag tgaagcgcgg caaggcgacg ggacggacgg
     2401 cgctgctcgg cggggcgctc gggctcgctt tcgcggccgc ccttctcggc cccggcgccc
     2461 tcgccgggct cgtcccggcg cgcggcgacc tcgcggactt cttctggccc atgaaggcgt
     2521 acacggccgc gcgctgggcg gccgggagcc ttccgctctg gaacccgctc tcgggctgcg
     2581 gcgagccgtg gctcgcgcag ctgcagacgg gcgtcctcta cccgggcgac cttccgttcc
     2641 tcctcggcgg cgccgcgggt cccctcctcg cgatcgccct gcatctttcg atcgcggcgg
     2701 ccggaatggc cgcgtggctc tcggacctcg gcacgtcccg cgcgggagcc ctcgccggcg
     2761 cggcgatctt cgccgggggc ggcgcgttcc tgtcgctcgc gcccgtgtac aacaacttct
     2821 gcacggcggc ctttctgccc tggctgtttc tcggggcgcg acgcgccgtg cgcggcgcgt
     2881 cgcccgcgcc cttcgcgctc gcggtggctc tcgcttttct cggcggcgag cccgcgctcg
     2941 cagccgtggg c
//
LOCUS       DAOMFY010000383         2928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_14707_length_2928_cov_6.367908, whole genome shotgun sequence.
ACCESSION   DAOMFY010000383 DAOMFY010000000
VERSION     DAOMFY010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14707_length_2928_cov_6.367908"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLB01_18945"
     CDS             complement(<1..965)
                     /locus_tag="PLB01_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79433.1"
                     /translation="MPRLSFALALGAAALLPAAAAATTSFSLPGIANTPGLNGTRFES
                     AVTLTNLGQTGGTVEIGLVTPGGVPAPAAVTRALAPGQTLPIPNALRDLFGLDGTFGT
                     LTIRSELPVDGRATTANTQSPAGTYGLGLPASGAGDVLLPGETGHALWVSHGPDTSRG
                     FRTNVNVTFLSADSAVTISVFDETGLFRGSRAVSSPAPAVWQASVADIAASADLPLGR
                     VEIAVARGSAIAYAAVVDNVTGDGIAATAVRVAPGPADWTLDGVALTFGANGTRWSTD
                     VRLFNPSAAPLAVTLDAVGFAAPVPSLTRTVPARGLLEIADVLGPS"
     gene            complement(998..2470)
                     /locus_tag="PLB01_18950"
     CDS             complement(998..2470)
                     /locus_tag="PLB01_18950"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HQQ79434.1"
                     /translation="MTRDTRLPGPPPPHGDFSPDDFRRMGEAAVERLARYFETLESRP
                     VLARTSPGEVLAQIPPRAPEQPEPWEAIERDLDRLVEPNLTHWQHPGYLAYFANTGTA
                     PAVVGDFLAAGYNQVGILWRTSPVLTELEQGVVRWLLDLVGLPSGPDGFDGQLADTAS
                     TASLIALAAAREEAYPDVRKTGLAGRPAGRVYSSELAHSSIDKACVVLGFGTEGLVKI
                     PADAAFRMDVEALASAISRDRAAGRVPVAVVATAGTTTVTSVDPLAALADLCARERVW
                     LHVDAAYAGSAAVLPEMRPLFAGWERADSVLYNPHKWMLVPLECTGLFFRRMERVRRA
                     FSVVPNYLETPEGQTVREYMDYGVQLGRRFRALKMWMTLRAFGAAGVRTRVADSLAGA
                     RVLKAALEREPHVSIVAPTPFSVVAFRWQPAGMDDAAADAANARILARINEDGRTFVS
                     HALVRGRYALRAAIGNARTEHRHLEAFLDAFRAAAAEERS"
     gene            complement(2484..>2928)
                     /locus_tag="PLB01_18955"
     CDS             complement(2484..>2928)
                     /locus_tag="PLB01_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79435.1"
                     /translation="DARRAGVAARADANRPGAARTAKKAIPLTLEWKGRASTPSPDPG
                     KPFWKLEVRIPHEELTFETESDAFVASLKIVVEAAAVDGPARDTASDDWFLSYASDEY
                     KAVRDTPAARLVTLQLPPGRYDLTVSVNDALGGTFGQATLRVDAR"
BASE COUNT          388 a         1033 c         1150 g          357 t
ORIGIN      
        1 gacgggccga gcacgtccgc gatctcgagg agcccgcgcg cgggcacggt gcgcgtcagg
       61 gacggcacgg gcgcggcgaa gccgacggcg tcgagggtga cggcgagcgg cgccgcggac
      121 gggttgaaca gccggacgtc cgtcgaccag cgcgtcccgt tcgccccgaa cgttagcgcg
      181 acgccatcca gcgtccagtc ggccggaccc ggggcaacgc ggacggccgt cgccgcgatc
      241 ccgtcgcccg tgacgttgtc cacgacggcc gcgtaggcga tcgcgcttcc gcgggccacg
      301 gcgatctcga cgcggccgag cgggaggtcc gcgctcgcgg cgatgtccgc gaccgaggcc
      361 tgccagacgg cgggcgccgg cgacgagacg gcgcggctgc cgcggaagag gcccgtctcg
      421 tcgaagacgg agatcgtgac ggcgctgtcc gcggagagaa acgtcacgtt gacgttcgtg
      481 cggaagccgc gggacgtgtc ggggccgtgc gacacccaga gcgcatggcc ggtctcgccg
      541 ggcaggagga cgtcgccggc gccggaggcc gggaggccga gtccgtacgt ccccgccgga
      601 gactgcgtgt tcgcggtcgt cgcccggccg tcgacgggca gctcgctccg gatcgtgagc
      661 gtcccgaacg tgccgtcgag gccgaagagg tcgcggagtg cgttggggat tgggagagtc
      721 tgtcccggag cgagcgcgcg cgtcacggcc gcgggcgccg gaacgccgcc cggcgtcacg
      781 aggccgatct cgaccgtccc gcccgtctgc ccgaggttcg tgagcgtgac cgcggactcg
      841 aatcgcgtcc cgttcagtcc cggggtgttc gcgattcccg gcagggagaa gctcgtggtg
      901 gcggcggccg cggccggaag cagggccgcg gctccgaggg cgagcgcgaa actcaggcgg
      961 ggcatcgccg gatgagacgt cgcgcccgcg cgggggttta cgaccgctcc tccgccgccg
     1021 cggcccggaa cgcgtcgagg aaggcctcga gatggcggtg ctcggtccgg gcgttcccga
     1081 tcgcggcgcg cagcgcgtac cggcccctca cgagggcgtg ggacacgaac gtccggccgt
     1141 cctcgttgat ccgggcgagg atccgcgcgt tcgccgcgtc ggccgcggcg tcgtccattc
     1201 ccgccggctg ccagcggaac gcgacgaccg agaacggcgt cggggcgacg atcgagacgt
     1261 gcggctcgcg ctcgagcgcg gccttcagca cgcgcgcgcc cgcgagcgag tcggcgacgc
     1321 gcgtcctcac gcccgcggcg ccgaacgcgc gcagcgtcat ccacatcttc agggcgcgga
     1381 agcggcgccc gagctggacg ccgtagtcca tgtactcgcg caccgtctgg ccctcgggcg
     1441 tctcgaggta gttcgggacg accgagaacg cgcggcgcac gcgctccatc cggcggaaga
     1501 agaggccggt gcactcgagc ggcacgagca tccacttgtg cgggttgtag aggacggagt
     1561 cggcgcgctc ccagcccgcg aagagcgggc gcatctccgg cagcaccgcg gccgaacccg
     1621 cgtacgcggc gtccacgtgc agccagaccc gctccctcgc gcagaggtcc gcgagcgcgg
     1681 cgagcgggtc gacggacgtg accgtcgtcg tcccggccgt cgcgacgacc gcgacgggaa
     1741 cgcggcccgc ggcgcggtcc cgcgaaatgg cggaggcgag ggcctcgacg tccatccgga
     1801 aggcggcgtc cgccgggatc ttcacgaggc cttccgtgcc gaagccgagc acgacgcagg
     1861 ccttgtcgat cgaggagtgc gcgagctccg aggagtagac ccggcccgcc ggccggcccg
     1921 cgaggccggt cttgcggacg tccgggtacg cctcctcgcg cgccgccgcg agcgcgatga
     1981 gcgaggccgt cgacgccgtg tccgcgagct ggccgtcgaa gccgtccggg cccgagggca
     2041 gccccacgag gtcgagcagc cagcgcacga cgccctgctc gagctccgtg aggacgggcg
     2101 acgtgcgcca gaggatcccg acctggttgt agccggcggc gaggaagtcg cccacgacgg
     2161 cgggcgccgt gcccgtgttc gcgaaatacg cgaggtagcc agggtgctgc cagtgcgtga
     2221 ggttcggctc gacgaggcgg tcgaggtcgc gctcgatcgc ctcccacggc tccggctgct
     2281 cgggcgcgcg cggcgggatc tgcgcgagga cctcgcccgg gctcgtgcgc gcgaggacgg
     2341 gacgcgattc gagggtctcg aagtaccgcg cgaggcgctc gacggccgcc tcgcccatcc
     2401 ggcggaagtc gtcgggggag aaatcgccgt gcggcggggg cgggccggga aggcgcgtgt
     2461 cccgggtcat ggccccagtc tactcagcgc gcgtcgacgc gcagcgtggc ctggccgaac
     2521 gtcccgccga gggcgtcgtt cacggagacg gtgaggtcgt agcggccggg cgggagctgg
     2581 agcgtcacga ggcgcgcggc cggcgtgtcg cgcacggcct tgtactcgtc gctcgcgtag
     2641 gagaggaacc agtcgtcgct cgccgtgtcg cgcgcgggcc cgtccaccgc cgcggcctcg
     2701 acgacgatct tcaggctcgc gacgaaggcg tccgattccg tctcgaaggt gagctcctcg
     2761 tgcgggatcc tgacctcgag cttccagaac ggcttgccgg ggtcgggcga gggggtcgag
     2821 gcgcgcccct tccactcgag cgtcagggga atcgctttct tcgccgtgcg cgccgcgccc
     2881 gggcggttcg cgtccgcccg cgccgcgacg ccggcgcggc gggcgtcc
//
LOCUS       DAOMFY010000384         2914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_14846_length_2914_cov_11.462399, whole genome shotgun
            sequence.
ACCESSION   DAOMFY010000384 DAOMFY010000000
VERSION     DAOMFY010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14846_length_2914_cov_11.462399"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1462
                     /gene="clpX"
                     /locus_tag="PLB01_18960"
     CDS             218..1462
                     /gene="clpX"
                     /locus_tag="PLB01_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQQ79436.1"
                     /translation="MTKKTGTGDVLRCSFCNKSQRDVKKLIAGPTVYICDECVDICLD
                     IIAEDRVLDQAKPETKLPKPREIKEFLDEYVVGQETAKRKLAVAVYNHYKRIDYFEKN
                     RKLDVELQKSNILLIGPTGTGKTLLAQTLAKMLSVPFTIADATTLTEAGYVGEDVENI
                     ILKLYQAAGQDKEKTERGIVYIDEIDKIARKGENPSITRDVSGEGVQQALLKILEGTI
                     TNVPPQGGRKHPHQEFIQIDTTNILFICGGAFVGLEDIISRRLTEKSMGFGAKIVSKK
                     EKRAGDTLEKVHPEDMIKFGMIPEFIGRVPVVATLHDLDRDALVAILKEPKNSLIKQY
                     QALLDFENVALRFTDDALESIADEAIVRNVGARGLKIILEELMLDIMYRVPSEEEIEE
                     CVITKDAVLRRAAPILSLRKAG"
     gene            1535..>2914
                     /locus_tag="PLB01_18965"
     CDS             1535..>2914
                     /locus_tag="PLB01_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQQ79437.1"
                     /translation="MTTPTSLEPAATAPRMLPLVPLRDMVVFPRMMAPFVVGRASSIA
                     ALEAALATPEKKIFLAAQKDPKVDEPAAGDIHETGVIASVVQSLKLPNGHIKVMVEGV
                     VRGVIRSFSPGVGHLAVTVEPLAPRPLGAASGDVGPYLSKVLAVFEQYAKLSHHLAFE
                     GLLSSLKMDDADAFCDMLAGHLPSSIPTPEKQTLLETVNPLERLQKLQDLLEIEIEKI
                     NIDRRINNKVKKQMERAQKEYYLNEKIKAIHQELGKKDDKSDEIEELRAKIEKAGMPK
                     ETKEKALAELKRLEAMPNVSAEATVSRNYIDWLVTVPWKKATREMKDIKRAEKILNED
                     HYGLEKVKERILEFLAVRQLVTETKGSILCFAGPPGVGKTSLAKSIAKSLNRKFVRLS
                     LGGVRDEAEIRGHRRTYIGAFPGQIIQLMKKAGTVNPVFLLDEVDKMSMDFRGDPSSA
                     LLEVLDPEQN"
BASE COUNT          571 a          999 c          926 g          418 t
ORIGIN      
        1 cctcggcgtg ggcgccggcc gccgcgagcg cggccaccag gagcgcggcc gccggggcgc
       61 gtcgggccgt ctgcatcatc tcgactgcgg ggatcatagg gtaggctccc cggtccccgg
      121 gggcgggcgg gaatgcccgg acgccggggt ttgttccgtg agaacccgag gcggtaagat
      181 gctgcctcag gacctttcag agggagccgg ggtagcgatg acgaagaaaa cgggcacggg
      241 cgacgtcctg cgctgcagct tctgcaacaa gtcccagcgc gacgtcaaga agctcatcgc
      301 ggggcctacg gtctacatct gcgacgagtg tgtggacatc tgtctcgaca tcatcgccga
      361 ggaccgggtt ctcgatcagg ccaagcccga gacgaagctc ccgaaacccc gtgagatcaa
      421 ggagttcctc gacgagtacg tcgtggggca ggagacggcc aagcgcaagc tcgccgtcgc
      481 cgtctacaac cactacaagc ggatcgacta cttcgagaag aaccgcaagc tggacgtcga
      541 gctccagaag tcgaacatcc tcctcatcgg gcccacgggg accgggaaga ccctcctcgc
      601 gcagacgctg gccaagatgc tctcggtccc gttcacgatc gccgatgcga cgacgctcac
      661 cgaggccggc tacgtcggcg aggacgtcga gaacatcatt ctcaagctct accaggccgc
      721 cggccaggac aaggaaaaga ccgagcgcgg gatcgtctac atcgacgaga tcgacaagat
      781 cgcgcgcaag ggcgagaacc cctcgatcac gcgcgacgtc tcgggcgagg gcgtccagca
      841 ggcgctcctg aagatcctcg agggcacgat cacgaacgtg cccccgcagg gcggccgcaa
      901 gcacccccac caggagttca tccagatcga cacgacgaac atcctgttca tctgcggagg
      961 cgcgttcgtc ggtctcgagg acatcatcag ccgccgcctg accgagaagt cgatggggtt
     1021 cggcgcgaag atcgtctcca agaaggagaa gcgggccggc gatacgctcg agaaggttca
     1081 tcccgaggac atgatcaagt tcgggatgat ccccgagttc atcggccgcg tgcccgtcgt
     1141 cgcgacgctg cacgacctcg accgcgacgc gctggtcgcg atcctcaagg agccgaagaa
     1201 ctccctcatc aagcagtacc aggcgctcct cgacttcgag aacgtggcgc tgcgcttcac
     1261 ggacgacgcg ctcgagtcga tcgcggacga ggcgatcgtg cgcaacgtgg gcgcgcgcgg
     1321 cctcaagatc atcctcgagg agctgatgct ggacatcatg taccgcgtcc cctccgagga
     1381 ggagatcgag gaatgcgtca tcacgaagga cgccgtcctg cgccgcgccg cgccgattct
     1441 ctcgctccgg aaggcgggct gaggtcgact ccctctccgg acgcgcgccc cggctcccgc
     1501 tctcctgccc ccactccgcc tccgaggtga cccgatgacg accccgacct ccctcgagcc
     1561 cgccgccacg gccccgcgca tgctcccgct cgttccgctc cgcgacatgg tcgtcttccc
     1621 gaggatgatg gcgccgttcg tcgtcggccg cgcctcgtcg atcgccgccc tcgaggccgc
     1681 gctcgcgact cccgagaaga agatctttct cgcggcgcag aaggacccga aggtcgacga
     1741 gcccgccgcg ggcgacatcc acgagacggg cgtcatcgcg tccgtcgtcc agtcgctcaa
     1801 gctcccgaac ggccacatca aggtcatggt cgagggcgtc gtgcgcggag tgatccgctc
     1861 gttctccccg ggcgtcggcc acctcgccgt cacggtcgag cccctcgccc cccgcccgct
     1921 cggcgccgcg tccggcgacg tcgggccgta tctctcgaag gtgctcgcgg tgttcgagca
     1981 gtacgcaaag ctctcgcacc acctcgcgtt cgaggggctc ctctcgtccc tcaagatgga
     2041 cgacgccgac gccttctgcg acatgctcgc cggccacctg ccgtcgtcca tcccgacgcc
     2101 ggagaagcag acgctcctcg agacggtcaa cccgctcgag cgcctccaga agctccagga
     2161 cctgctcgag atcgagatcg agaagatcaa catcgaccgc cggatcaaca acaaggtcaa
     2221 gaagcagatg gagcgggcgc agaaggagta ctacctcaac gagaagatca aggcgatcca
     2281 ccaggagctc ggcaagaagg acgacaagtc cgacgaaatc gaggagctcc gcgccaagat
     2341 cgagaaggcc gggatgccga aggagacgaa ggagaaggcg ctcgccgagc tcaagcgtct
     2401 cgaggcgatg ccgaacgtct ccgccgaagc gacggtctcg cgcaactaca tcgactggct
     2461 cgtgacggtg ccctggaaaa aggcgacgcg cgagatgaag gacatcaagc gcgccgaaaa
     2521 gatcctgaac gaggaccact acgggctcga gaaggtcaag gagcgcatcc tcgagttcct
     2581 ggccgtgcgc cagctcgtca cggagacgaa gggctccatc ctgtgcttcg cgggccctcc
     2641 cggcgtcggc aagacgtcgc tcgcgaagag catcgcgaaa tcgctgaacc gcaagttcgt
     2701 ccgactgtcg ctcggcggcg tgcgcgacga ggccgagatc cgcggccacc ggcgcacgta
     2761 catcggcgcg ttcccgggcc agatcatcca gctcatgaag aaggcgggga ccgtgaaccc
     2821 cgtgttcctc ctcgacgagg tcgacaagat gtcgatggac ttccgcggcg acccgtcgtc
     2881 ggcgctcctc gaggtcctcg accccgagca gaac
//
LOCUS       DAOMFY010000385         2901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_14982_length_2901_cov_4.269852, whole genome shotgun sequence.
ACCESSION   DAOMFY010000385 DAOMFY010000000
VERSION     DAOMFY010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14982_length_2901_cov_4.269852"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2406
                     /locus_tag="PLB01_18970"
     CDS             <1..2406
                     /locus_tag="PLB01_18970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ79438.1"
                     /translation="GAPELEPWLPAGERTHNDDLARALWVRGADEAFPELGDMLTIRS
                     PFESTTSSFGVMRPGSRAPDAVIPVSIPSGFPATFLHVPWPREADRDPLLSFVASREV
                     PDRVAAERLEWDASGRPSGLRGENVELPTSFLAAVRRNGRAVGTVSTSDGPRRVHVSS
                     APDGFSGFAAPVDPALTSVGAAVAAGEAALVVLVPLLFAFRPARQDRARGPRFFGTFR
                     ARLVALVVLFGALPLAASVVLVRVSLANHATRETGRRARDLVTEGRRVLQSGEEGLAT
                     SPEGELNRAAAVIGTDLLRYRDGTLVAASRALPVSAAIARERLSAAVAQGLAEGRPAS
                     VAARRTNTPGAPRVFEAAEALSRDGRDVLAVVVGEDEALRSAADSLVLLSVAVVLLAL
                     GLGGRAALALGQPLEDLLEATGKVGEGRALPPIRRPKNEDLARLIDAFGQMSARVEER
                     TQSLARERASAVGLLENLTAAVLLYRRKDGAVLLSNEAADQILPGGSLAERLDGSEWA
                     PLRAALDAAASRPSPYETRVAVPGPSGERLFRVAIVTLPEDLGEGRAILLLEDLTEFT
                     RADRLAAWVDAARAIAHDIKNPLTPIRLAAERLGRLEERPVPPSPGAFAEISANILRQ
                     VAILTERIGRLGRFGDPAALDRRTLDAAAVRALLEEVAADFRAHERLTVTVDVPAGLR
                     SLAADRLLLRDALTNFLVNAAEALAECGGAVVLSASNAALTGGAAAVRFACADDGPGA
                     PEGAAERLFEPAFSTKARGSGMGLAAVRRTAERHGGEVFAEARPGGGLVIGFTLPALS
                     SPA"
     gene            2403..>2901
                     /locus_tag="PLB01_18975"
     CDS             2403..>2901
                     /locus_tag="PLB01_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79439.1"
                     /translation="MNSGSRRRGARAAFRTGVPPFTLLAALLASSLVLSGGCRKSAEP
                     AGAPRLSRVACRADALFLAETPAYIPDEADAELSGMGIARVYVVAATLGRDGRATPAP
                     PPPAPLKRPIVLVLMGSEDAASALTGRGAEAGAEWARAVARVLADSRSWGRVAGVHVH
                     IWPTPE"
BASE COUNT          307 a         1094 c         1094 g          406 t
ORIGIN      
        1 ggcgcgccgg agctcgagcc gtggctgccg gccggcgagc ggacgcacaa cgacgacctc
       61 gcgcgcgccc tctgggtgcg cggcgccgac gaggcctttc cggagctcgg cgacatgctg
      121 acgatccgca gcccgttcga gtcgacgacg tcctcgttcg gcgtcatgcg acccgggagc
      181 cgcgcgccgg acgcggtgat tccggtctcg atcccgtctg gtttcccggc gacgttcctg
      241 cacgtgccct ggccgcgcga ggcggaccgc gacccgctcc tgtccttcgt cgcctcgcgc
      301 gaggtgcccg atcgcgtggc ggccgagcgc ctcgagtggg acgcctcggg ccggccgtcc
      361 gggctgcgcg gcgagaacgt cgagcttccg acctcctttc tcgcggcggt ccgccgcaac
      421 ggaagggccg tcggcacggt atcgacgagc gacgggccgc gccgggttca cgtctcctcg
      481 gctccggacg gcttctcggg cttcgccgcg ccggtcgatc cggccctgac ctccgtgggc
      541 gcggcggtcg cggccggcga ggccgcgctc gtcgtcctgg tcccgctcct gttcgccttc
      601 cgtcccgcgc ggcaggaccg tgcgcggggg ccgcgtttct tcggcacgtt ccgcgcgcgc
      661 ctcgtcgcgc tcgtcgtcct gttcggggcg ctcccgctgg cggcgagcgt cgttctcgtc
      721 cgcgtctccc tcgcgaacca cgcgacacgt gaaaccggcc ggcgcgcgcg cgacctcgtg
      781 acggaggggc gccgcgtcct ccagagcggg gaggaggggc tcgcgacgtc gcccgagggc
      841 gaactgaacc gcgcggcggc cgtcatcggc acggacctcc ttcgttaccg cgacgggacg
      901 ctcgtggcgg cgtcgcgcgc cttgcccgtt tcggcggcga tcgcgcgcga gcggctgtcg
      961 gcggcggtgg cgcagggtct cgccgagggg cgtccggcct ccgtggccgc gcgccggacg
     1021 aacaccccgg gcgcgccgcg ggtcttcgag gccgccgagg cgctctcgcg cgacggacgc
     1081 gacgtcctgg cggtcgtcgt cggggaagac gaagcgctcc gctccgcggc ggattccctc
     1141 gttcttctct cggtcgccgt ggtgctcctc gcgctgggcc tcggaggccg tgcggcgctc
     1201 gcgctcggcc agcctctcga ggacctcctc gaggccaccg ggaaggtcgg cgagggccga
     1261 gcgcttccgc cgatccgccg cccgaagaac gaagacctcg cgcgcctcat cgacgcgttc
     1321 gggcagatgt cggcgcgcgt cgaggagcgc acgcagtcgc tcgcgaggga acgggcgtcg
     1381 gccgtaggcc tgctcgagaa cctcacggcg gccgtcctcc tctatcgtcg caaggatggg
     1441 gcggtcctcc tgtcgaacga ggcggcggac cagatcctgc cgggcgggag cctcgcggag
     1501 cggctcgacg gctccgagtg ggcgccgctc cgcgcggcgc tcgacgcggc cgcctcgcgt
     1561 ccgtcgccct acgagacgcg cgtcgcggtg ccgggaccgt cgggagagcg cctcttccgg
     1621 gtcgcgatcg tcacgctgcc ggaggatctc ggggaggggc gggcgatcct gctcctcgag
     1681 gacctcacgg agttcacgcg cgcagaccga ctcgcagcgt gggtggacgc cgcgcgtgcg
     1741 atcgcgcacg acatcaagaa cccgctcacg cccatccgcc tcgccgcgga gaggctggga
     1801 aggctcgagg agcgccccgt accgccgtcg ccgggcgcgt tcgcggagat ctccgcgaac
     1861 atcctgagac aggtggcgat cctcaccgag cgcatcggga ggctcggccg tttcggagac
     1921 ccggcggcgc tcgaccgccg gacgctcgac gcggcggcgg tccgcgcgct cctcgaggag
     1981 gtcgcggccg acttccgggc gcacgagcga ctgaccgtga cggtcgacgt gccggccggc
     2041 ctcaggtctc ttgcggccga caggctgctt ttgcgcgacg cgctcacgaa cttcctcgtg
     2101 aacgccgccg aggccctcgc cgagtgcgga ggcgcggtcg tcctctcggc ttcgaacgcc
     2161 gcgctgaccg gaggcgccgc ggccgtgcgc ttcgcctgcg cggacgacgg accgggcgcg
     2221 ccggagggcg ccgcggaacg gctcttcgag cccgcgttct ccacgaaggc gcgcggttcc
     2281 ggcatgggcc tggcggccgt gcgccgcacc gccgagcggc acggcgggga agtcttcgcc
     2341 gaggcccggc ccggcggggg actcgtgatc ggcttcaccc tgcccgcgtt atcatcgccg
     2401 gcgtgaacag cggttcccga cgccgcggcg cccgcgccgc gttccggacg ggcgtaccgc
     2461 ccttcacgct cctcgccgcc ctgctcgcgt cgtctctcgt cctctccggc ggctgccgga
     2521 agagcgcgga gccggctggc gctccgcgcc tctcgcgggt agcatgccgg gccgatgccc
     2581 tgttcctcgc ggagaccccg gcgtacatcc cggacgaggc ggacgccgag ctctcgggca
     2641 tgggcatcgc gcgcgtctac gtcgtggccg cgacgctggg acgcgacggc cgcgcgacgc
     2701 cggcgccgcc gccgcccgcg ccgctgaagc ggccgatcgt gctcgtcctc atgggttcgg
     2761 aggacgcggc gtccgcgctc acgggacgcg gcgccgaggc gggggccgaa tgggcgcggg
     2821 ccgtcgcgcg cgtgctcgca gattccagga gctggggccg cgtggcgggc gtgcacgtgc
     2881 acatctggcc gactcccgag c
//
LOCUS       DAOMFY010000386         2882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_15150_length_2882_cov_9.218960, whole genome shotgun sequence.
ACCESSION   DAOMFY010000386 DAOMFY010000000
VERSION     DAOMFY010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15150_length_2882_cov_9.218960"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLB01_18980"
     CDS             complement(<1..258)
                     /locus_tag="PLB01_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79440.1"
                     /translation="MPSRPATSVVPYAYRAGVLSLLDQRRLPHEETVLECASALETAE
                     AIRSLAVRGAPLIGVAAAYGMCAEARRVLPRVPEDLDAALVA"
     gene            327..1520
                     /locus_tag="PLB01_18985"
     CDS             327..1520
                     /locus_tag="PLB01_18985"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQQ79441.1"
                     /translation="MADLPPSITALDLDGKRVFLRVDFNVPMKDGVVRDDTRVVEALP
                     TLLLARERGARRIVLASHLGKAKGSPDPKYSLAPVARTLEHRLGAPVAFAPDCVGPEA
                     EKAEAALPPGGVLLLENVRFHKGEEANDAVFSKQLASLGEVYVNDAFGTAHRAHASTA
                     GMAAFFAPGRRGIGLLMEKELTALAKVATDIDHPFAAILGGAKIMGKIAALKTLVEKA
                     DVVLVGGGMANHFVAALGLPVGRSLLEPEGVPIARQIIDRCKERGVALVLPSDFMVAP
                     SLEKASAAKAVAMNAIPADQAALDVGPKTLEMFRRMTKDARTIFWNGPMGVFETPPFD
                     AGTLGVAKLMAASPAFTVVGGGESVEAIHKAGLGASISHVSTGGGASLDLVAGETLPG
                     VEALR"
     gene            1526..2275
                     /gene="tpiA"
                     /locus_tag="PLB01_18990"
     CDS             1526..2275
                     /gene="tpiA"
                     /locus_tag="PLB01_18990"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQQ79442.1"
                     /translation="MAARRLVVANWKMSLTPSASLALAERLLDARLPPGVDVGLAPSF
                     GALERVGRRLRSSRIALASQDVSPEAQGAFTGEVSAASLKELGVSIGIVGHSERRQRH
                     GEAGPLLARKIERLVENGIAPLYCVGETRAERDAGRTHAILTGQLGALDGFAAPPPGF
                     ALAYEPVWAIGTGLAATPAMAAEAHAVLREALGTRWGVAAAASIRILYGGSVTPANAP
                     ELFGQAGVDGGLVGGASLDAAAFGAILRAAA"
     gene            2390..2764
                     /gene="secG"
                     /locus_tag="PLB01_18995"
     CDS             2390..2764
                     /gene="secG"
                     /locus_tag="PLB01_18995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQQ79443.1"
                     /translation="MWLYVLVPIYVIVCVFLILVVLLQQGKGADVSAAFGASSSQTTF
                     GARGATTLLEKITTWSFVAFSVLAVTISLIQARRPGSVLAKGAKSVAPAKASVPAAPA
                     PVTALPAAPVAPVTAPAAPATK"
     gene            2798..2882
                     /locus_tag="PLB01_19000"
     tRNA            2798..2882
                     /locus_tag="PLB01_19000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2832..2834,aa:Leu,seq:gag)
BASE COUNT          369 a         1059 c         1040 g          414 t
ORIGIN      
        1 ggcgacgagc gccgcgtcga ggtcctccgg cacgcgcggc agcacacgcc gcgcctccgc
       61 gcacatcccg taggccgccg cgacgccgat gagcggggcc ccgcggacgg cgaggctgcg
      121 gatcgcctcg gccgtctcga gggcgctcgc gcactcgagg acggtttcct cgtgcggcag
      181 gcggcgctgg tcgagaagcg agagcacgcc cgcgcggtac gcgtacggca cgacggacgt
      241 cgcggggcga gacggcatct ccacggcccg actataagcg cccggtcggt taggatcgtg
      301 ccgcggtcac caagcaggag gaccccatgg cggaccttcc tccctcgatc acggccctcg
      361 acctcgacgg caagcgcgtc ttcctccgcg tggatttcaa cgtcccgatg aaggacggcg
      421 tcgtgcgcga cgacacgcgc gtcgtcgaag cgctgccgac cctccttctc gcgagggaac
      481 gcggcgcgcg ccgcatcgtc ctcgcgtcgc acctcggcaa ggccaagggc tccccggacc
      541 cgaagtactc gctcgcgccc gtggcccgga cgctcgagca ccggctcggc gcccccgtgg
      601 ccttcgcgcc cgactgcgtc ggccccgagg cggagaaggc cgaggccgcc ctgccccccg
      661 gcggcgtcct cctcctcgag aacgtgcggt tccacaaggg cgaggaggcg aacgacgccg
      721 tgttctcgaa gcagctcgcg tcgctgggcg aggtctacgt gaacgacgcg ttcgggaccg
      781 cccaccgcgc gcacgcctcg acggccggga tggcggcttt cttcgcgccg ggccggcgcg
      841 ggatcggcct cctcatggag aaggagctca cggccctcgc gaaggtcgcg acggacatcg
      901 accacccgtt cgcggcgatc ctcggcggcg cgaagatcat ggggaagatc gccgcgctga
      961 aaacgctcgt cgagaaggcg gacgtcgtcc tcgtgggcgg cgggatggcg aaccacttcg
     1021 tcgcggcgct cggactgccc gtcggcaggt cgctgctcga gcccgagggc gtcccgatcg
     1081 cgcggcagat catcgaccgc tgcaaggagc gcggcgtcgc gctcgtcctc ccgtccgact
     1141 tcatggtcgc gccgtcgctc gagaaggcct ccgcggcgaa agcggtcgcg atgaacgcga
     1201 tccccgccga ccaggccgcg ctcgacgtcg gcccgaagac gctcgagatg ttccggcgca
     1261 tgacgaagga cgccaggacg atcttctgga acggtccgat gggggtcttc gagacgccgc
     1321 cgttcgacgc ggggaccctc ggcgtcgcga agctcatggc ggcttcgccg gcgttcacgg
     1381 tcgtgggcgg cggcgagagc gtcgaggcga tccacaaagc cgggctcggg gcctcgatct
     1441 cgcacgtctc gacgggcggc ggcgcctcgc tcgacctcgt cgcgggcgaa acgctgccgg
     1501 gcgtcgaggc gctgcggtga cgccgatggc cgcgcgccgg ctcgtcgtcg cgaactggaa
     1561 gatgtccctc acgccgtcgg cgtcgctcgc cctcgccgag cgccttctcg acgcgcgtct
     1621 cccgccgggc gtcgacgtcg gcctcgcccc gtcgttcggc gcgctcgagc gcgtcggccg
     1681 ccgcctccgg agctcgcgga tcgcgctcgc ctcccaggac gtctcgcccg aggcgcaggg
     1741 cgcgttcacg ggagaggtct cggcggccag cctgaaggag ctcggggtct cgatcggcat
     1801 cgtcgggcac agcgagcgcc gccagcgcca cggggaagcg ggccccctcc tcgcgcggaa
     1861 gatcgagcgc ctcgtcgaga acgggatcgc gccgctctac tgtgtcggag agacgcgtgc
     1921 cgagcgcgat gcggggcgga cgcacgcgat cctcaccggc cagctcggtg ccctcgacgg
     1981 cttcgcggcc ccgccgccgg gtttcgcgct cgcgtacgag ccggtctggg cgatcggaac
     2041 gggcctcgct gcgacgccgg ccatggcggc cgaggcccac gcggtcctgc gggaggccct
     2101 gggcacgcgc tggggggtgg cggcagccgc ctcgatccgg atcctctacg gcgggtccgt
     2161 cacgcctgca aacgcgccgg agctcttcgg ccaggccggc gtcgacgggg ggctcgtggg
     2221 aggggcgtcg ctggacgcgg cggcgttcgg ggcgatcctg cgggcggccg catagggcgg
     2281 ccagtcccct ttcgaccccg aaagtcgcgc ccggccggtg tttccgctat catctgcggc
     2341 cccgccgagc tcgtccgcga gcccagtggc ggggtccgga gttcaccgca tgtggctgta
     2401 cgttctcgtc ccgatctacg tgatcgtctg cgtgttcctg atcctcgtcg tcctgctcca
     2461 gcagggcaag ggcgcggacg tctcggcggc cttcggggcc tcctcctcgc agacgacctt
     2521 cggcgcccgc ggcgcgacga cgcttctcga gaagatcacg acgtggtcct tcgtggcgtt
     2581 ttcggtcctc gcggtcacca tttccctgat ccaggcgcgg cgtccgggct ccgtgctcgc
     2641 gaagggcgcg aagtcggtcg cccccgcgaa ggcgtccgtc cccgctgccc cggcgcccgt
     2701 gaccgcgctc ccggccgcgc ccgtggctcc ggtcacggct cccgcagctc ccgcaacgaa
     2761 gtagacatcg gttaagatcc ccctccctcg cgagggtgcc ctggtggcgg aattggtaga
     2821 cgcgcacgtt tgaggggcgt gtggagcaat ccgtgccagt tcgagtctgg cccagggcac
     2881 ca
//
LOCUS       DAOMFY010000387         2879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_15174_length_2879_cov_7.273017, whole genome shotgun sequence.
ACCESSION   DAOMFY010000387 DAOMFY010000000
VERSION     DAOMFY010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15174_length_2879_cov_7.273017"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLB01_19005"
     CDS             complement(<1..1254)
                     /locus_tag="PLB01_19005"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQQ79444.1"
                     /translation="MRRTLAAASAATLLAFGVPTRLDAADDVSVQVNVDKARPSADEF
                     VHLTYTFTGGGLSGTLRVPSPIPLRNLSVAGGPSRSEQVSYVNGVFSRTLSITYVLRP
                     LGPGPAEIGETSFGFGDKTVKAASYLLEVGAARPASSSPRAAQQESEPEDPLSQFFRR
                     RDAGGVPRAERGARPFVEFRVTADKTTAYVGEEITLTYELLTQADVQGLEYIEPPKFP
                     GLWAEDLEKPERPEGRRDVVDGRTVMRFTLLKKAVSGLTPGAVTLPEAKIRTSVRVGP
                     DPFGDPFGFFPRPQVLDLVAKPVNLKILAVPGRPDFKGPVGRFELTAKIDRTRVAAGD
                     AATFKVRLSGTGNLRTASEPPRLEVAGAKVYPPSTKTDPSHLGRTQTSTEWSWVLVPT
                     AKGSVTIPPVSVDVFDPVEKRVVAKA"
     gene            complement(1251..>2879)
                     /locus_tag="PLB01_19010"
     CDS             complement(1251..>2879)
                     /locus_tag="PLB01_19010"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ79445.1"
                     /translation="LDTSASMRAADVSPSRFALARQAAQSLVARLGSDRVALVSCEGD
                     AQALVPLTLDAAAVGLFLDAIEPGMGAKPGTSIAAGIAAAAELFPPGVGGGRHLVFFS
                     DGEDLEGGVDAAIGRAKAEGITIHAVFVGAVSGTGAPVPEVDAAGRTSGYKSDAGGQP
                     VLSKPDPDLLRRLAAETGGSYTAVTSLRTDLEGVARQIDLGARRPMSEVLLTMREERF
                     QIPLAVAVAALGLLLLGVGAGRKRKEKRRFLRKVRGAETVATAALLVMGTAPRAAAQS
                     APAVPVSAAPQQAAPSPSSPSKNSSPSSDPAAATSGSPEEPAPLPLSKKIFSSPRSSA
                     QKGQKALDEKKTDEALTHFQEEIALDPKDPTGAYNLGTAHSKAGRVPEALASLERARK
                     EGSGALAADAAYNEGQALFREKQYEKAAAAFRDALRRRPGDGDAAWNYELCVRRAEEQ
                     KKQQEQQKKDQQKNEKKDKKDQQGKDDKQKQEPQQAGKDEEKRRQDREFEQKANMSRE
                     KAEQLLSAIERSDLDEQKKKIAEQRSRRKVARDW"
BASE COUNT          377 a         1009 c         1030 g          463 t
ORIGIN      
        1 cgccttcgcg acgacgcgct tctcgacggg gtcgaagacg tcgacggata caggcggaat
       61 cgtgacgctc cccttcgcgg tgggcacgag gacccagctc cactcggtcg aggtctgcgt
      121 tcgcccgagg tgcgacgggt ccgttttcgt gctcgggggg tagaccttcg cgcccgccac
      181 ctccagtcgg ggaggctcgg acgccgtgcg gaggttcccc gtcccggaaa gccgcacctt
      241 gaacgtggcg gcgtcaccgg ccgcgacgcg cgtccggtcg atcttcgccg tgagctcgaa
      301 acgacccacg ggtcccttga agtcgggacg gccggggacc gcgaggatct tcaggttgac
      361 gggcttggcc acgaggtcca gcacctgcgg cctcgggaag aacccgaacg gatctccgaa
      421 cgggtcgggg cccacgcgga cgctcgtgcg gatcttggcc tcgggcagcg tgacggcgcc
      481 cggcgtcagg ccggacacgg ctttcttgag gagcgtgaag cgcatcacgg tgcgcccgtc
      541 gacgacgtcg cggcggccct ccggccgctc cggcttctcg aggtcctctg cccagagccc
      601 cgggaacttc ggcggctcga tgtactcgag cccctggacg tcggcctggg tgaggagctc
      661 gtacgtcagc gtgatctcct cgccgacata cgccgtcgtc ttgtccgccg tgacgcggaa
      721 ttcgacgaag gggcgcgcgc cgcgctcggc tcgggggact ccccccgcgt cgcgccggcg
      781 gaagaactgg gagagcgggt cctcgggctc ggactcctgc tgcgccgcgc gcggcgagga
      841 gctcgcaggc cgagccgctc cgacttcgag gagataactc gccgccttca cggtcttgtc
      901 gccgaacccg aacgacgtct ccccgatctc ggcgggcccg ggccccaggg gccgcaggac
      961 gtacgtgatc gagagcgtcc ggctgaacac gccgttcacg taggagacct gctccgagcg
     1021 ggacgggccg ccggcgacgg acaggttccg gagcgggatc ggcgagggga cgcgcagcgt
     1081 gccggagagc ccgccccccg tgaacgtgta cgtgaggtgc acgaactcgt ccgcggacgg
     1141 ccgtgccttg tccacgttca cctgcacgga gacgtcgtcg gcggcgtcga ggcgcgtggg
     1201 cacaccgaat gcgagaagcg tcgcggcgga cgcggcggcg agagtgcgcc tcaccagtcc
     1261 ctcgcgacct tccgccggct gcgctgctcg gcgatcttct tcttctgctc gtcgaggtcc
     1321 gagcgctcga tggcggagag gagctgctcc gccttctcac gcgacatgtt cgccttctgc
     1381 tcgaactcac gatcctggcg gcgcttctcc tcgtccttgc cggcctgctg cggctcctgc
     1441 ttctgcttgt cgtccttgcc ctgctggtcc ttcttgtcct tcttctcgtt cttctgctgg
     1501 tccttcttct gctgctcctg ctgcttcttc tgctcctcgg cccggcggac gcagagctcg
     1561 tagttccacg cggcgtcccc gtcgcccggg cggcgccgca gcgcgtcccg gaaggccgcg
     1621 gccgccttct cgtactgctt ctcgcggaag agcgcctggc cctcgttgta cgcggcgtcg
     1681 gccgcgagcg cgccgctccc ttccttgcgc gctctttcga gcgacgcgag cgcctcggga
     1741 acgcgccccg ccttcgaatg cgcggtgccg aggttgtacg cgcccgtcgg gtccttcggg
     1801 tcgagcgcga tctcctcctg gaagtgcgtc agggcctcgt cggtcttctt ctcgtcgagc
     1861 gctttctgcc ccttctgggc cgaggaacgg ggggaagaga agatcttctt tgaaagaggg
     1921 aggggagccg gctcctctgg ggagcccgag gtggcagcgg cagggtccga agagggcgaa
     1981 gagttcttag aaggtgaaga gggagaagga gcagcctgct gcggcgccgc ggataccggc
     2041 acggcgggcg ccgattgagc cgccgcgcgg ggcgccgtac ccatcacgag cagggccgcc
     2101 gtggccacgg tctcggcgcc tcttaccttt ctaagaaatc ttcttttctc ttttctcttc
     2161 ctccccgctc cgaccccgag cagcaggagc ccgagcgccg ccaccgcgac ggcgagcggg
     2221 atctggaagc gctcctcgcg catcgtgaga aggacctcgg acatcgggcg gcgcgcgccg
     2281 aggtcgatct ggcgggccac accctcgagg tcggtccgga gcgacgtgac ggcggtgtac
     2341 gacccgcccg tctcggcggc gaggcggcgg aggagatccg ggtcgggctt cgagaggacc
     2401 ggctggccgc cggcgtcgga cttgtagccg ctcgtgcggc ccgcggcgtc cacctcggga
     2461 acgggcgctc ccgtcccgga cacggcgccg acgaagacgg cgtggatcgt gatcccttcc
     2521 gccttcgcac ggccgatcgc ggcgtcgacg ccgccctcga ggtcctctcc gtccgagaag
     2581 aagacgaggt gacggccgcc gccgacgccc ggagggaaca gctcggccgc cgccgcgatg
     2641 cccgccgcga tcgacgtgcc gggcttggcg cccatgccgg gctcgattgc gtccaggaag
     2701 aggccgacgg cggccgcgtc gagcgtgagc ggcacgagcg cctgtgcgtc gccctcgcac
     2761 gagacgagcg cgacgcggtc gctgccgagg cgcgcgacga gggactgcgc ggcctggcgg
     2821 gcgagcgcga aacgcgacgg cgagacgtcc gccgcgcgca tcgacgcgga cgtgtcgag
//
LOCUS       DAOMFY010000388         2869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_15264_length_2869_cov_6.935323, whole genome shotgun sequence.
ACCESSION   DAOMFY010000388 DAOMFY010000000
VERSION     DAOMFY010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15264_length_2869_cov_6.935323"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1475)
                     /locus_tag="PLB01_19015"
     CDS             complement(57..1475)
                     /locus_tag="PLB01_19015"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQQ79446.1"
                     /translation="MKAFALCLFASALAAPLASAPPLLTDGMRADVASLVASGKATSG
                     AYETIRELLEAAGPRLAGSPGDVLAVAWAQRALRARGFVNVHTEPVTAPHWERGEERG
                     DLLLPIPLRLSLCALGGSVGTPPEGVEGDVVEATSLEGVDALGEKAKGKIVLIWKVME
                     RGSEGSGYGATVPIRSNGASRAAKGGALAVVIRSVGTSDARFPHTGALNYAEGVAKIP
                     AAALAVHDADLLHSYLSKGMRVRLKMALGARTLPDVQTANVVGEIPGREKPEEIVLVG
                     GHLDSWDLGMGALDDGAGCGIAIESARLVGKLPRRPRRTIRVVLFANEENGLAGGRAY
                     AKAHADELGRHVAAVETDAGAGRVKGFAWSAGPATEPLVTELGAALAPFGLGEVKKGG
                     GGADIGPMRAAGVPMFSPLQDSSRYFDVHHTADDTFDKIDREELNTGVSTIAALLYTL
                     AETDAPIEKIPEAERERRRRAP"
     gene            complement(1472..2380)
                     /gene="ftcD"
                     /locus_tag="PLB01_19020"
     CDS             complement(1472..2380)
                     /gene="ftcD"
                     /locus_tag="PLB01_19020"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HQQ79447.1"
                     /translation="MARLIECIPNVSEGRRDEVVAAIAAAAVKAAPGVVLLDRTSDSD
                     HNRSVLTFLGDGEPLVAAMTALVEASLAAIDLRTHTGAHPRLGAVDVIPFVPVRGATS
                     AECVELAKTLGKSLAERFGLPVYLYEDAAATPERQNLAAIRKGEFEGLAKKMEDPAWT
                     PDFGPAAPHPSGGAVVVGARSPLIAYNINLATADLGVADRIAKAIRHLSGGYRFVKAM
                     GVKLEARGQVQVSINMTNFEKTPLHRVFETVRSEAERHGVAVVGSEIVGLVPQAALLA
                     AAGHYLRLEADPGPQVLENKLLERLS"
     gene            2448..>2869
                     /locus_tag="PLB01_19025"
     CDS             2448..>2869
                     /locus_tag="PLB01_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79448.1"
                     /translation="MASYAGSAALSAEAREKVLQTFRHTLSLAQSGKADEALLGCDFI
                     LKMDGRFAPARALLDALRTAAPGAPVDVNRFSEFLGFESAVEELPGSAELPAVPKSAP
                     APPLRDAFPGPAPSAPQAGMDDLVFGDEPAHAPQAAAAP"
BASE COUNT          374 a         1063 c         1019 g          413 t
ORIGIN      
        1 tccctcggct accgtccacc ggcatccagg gtgaacaacc tgtttagcct cgacaactag
       61 ggcgcgcgcc tccggcgctc acgctcggcc tccgggatct tctcgatcgg ggcgtccgtc
      121 tccgcgaggg tgtagaggag ggccgcgatc gtcgagacgc cggtgttgag ctcctcgcgg
      181 tcgatcttgt cgaacgtgtc gtccgccgtg tggtggacgt cgaagtagcg cgaggagtcc
      241 tgcagcggcg agaacatcgg gacgcccgcg gcgcgcatcg ggccgatgtc ggcgcccccg
      301 ccgccctttt tcacctcgcc gagcccgaac ggcgcgagcg ccgcgcccag ctccgtcacg
      361 agcggctccg tcgccgggcc cgcgctccag gcgaagccct tcacgcggcc ggcgccggcg
      421 tccgtctcga cggccgcgac gtgcctgccg agctcgtccg cgtgggcctt cgcgtaggcc
      481 cggccgcccg cgaggccgtt ctcctcgttc gcgaagagga cgacgcggat cgtccgccgc
      541 ggacgccgcg ggagcttccc gacgaggcgc gcggactcga tcgcgattcc gcaccccgcc
      601 ccgtcgtcga gggcgcccat cccgagatcc cacgagtcga ggtggccgcc gacgaggacg
      661 atttcttcgg gcttctcgcg gcccgggatc tcgcccacga cgttcgccgt ctgcacgtcg
      721 gggagcgttc gcgcgccgag ggccatcttc aggcgcacgc gcatcccctt cgacaggtac
      781 gaatggagga ggtccgcgtc gtggaccgcg agggccgccg ccgggatctt cgcgacgccc
      841 tccgcgtagt tcagcgcgcc cgtgtgcggg aagcgcgcat cggacgtgcc caccgaccgg
      901 atgacgaccg cgagggcgcc acccttcgcg gcgcgcgagg cgccgttcga gcggatcggc
      961 accgtcgcgc cgtagcccga gccctcgctc ccgcgctcca tgaccttcca gatcaggacg
     1021 atcttcccct tcgccttctc gccgagcgcg tcgacgccct ccagcgacgt cgcctcgacg
     1081 acgtcgccct cgacgccctc cggcggagtc ccgacgctgc cgccgagcgc gcagagcgag
     1141 aggcggagcg ggatcgggag gagaaggtcc cccctctcct cgccgcgctc ccagtgcggc
     1201 gccgtcacgg gctccgtgtg aacgttgacg aagccgcgcg ccctgagcgc acgctgggcc
     1261 cacgcgacgg cgaggacatc gccgggcgag cccgcgaggc gcgggccggc cgcctcgagc
     1321 agctcgcgaa tcgtctcgta cgcgccagac gtggctttcc ccgacgcgac gagcgacgcg
     1381 acgtccgcgc gcatgccgtc cgtcagcagc ggcggggccg acgcgagggg ggccgcgagc
     1441 gccgaggcga aaaggcacag ggcgaacgct ttcatgaaag cctttcgagg agcttgtttt
     1501 cgaggacctg cgggccgggg tccgcctcga ggcgcaggta gtggccggcg gccgcgagga
     1561 gcgcggcctg cgggacgagg ccgacgatct ccgagccgac gaccgcgact ccgtgccgct
     1621 cggcctcgga acgcaccgtc tcgaagacgc ggtggagcgg agtcttctcg aagttcgtca
     1681 tgttgatcga gacctgcacc tggccgcgcg cctcgagctt cacgcccatc gccttcacga
     1741 agcggtagcc gcccgagagg tggcggatcg ccttcgcgat gcggtcggcg acgccgaggt
     1801 cggcggtcgc gaggttgatg ttgtacgcga tgagcggcga acgcgcgccg acgacgacgg
     1861 cgcccccgga cggatggggc gccgcgggtc cgaagtcggg cgtccacgcg gggtcttcca
     1921 tcttcttcgc gagcccctcg aactccccct tgcggatggc cgcgaggttt tggcgctcgg
     1981 gcgtcgccgc ggcgtcctcg tagaggtaga cgggcaggcc gaagcgctcg gcgagcgact
     2041 tcccgagcgt cttcgcgagc tcgacgcatt cggcgctcgt ggcgccgcgc acggggacga
     2101 acgggatgac gtcgacggcg ccgaggcgcg ggtgcgcgcc cgtgtgcgtc ctgaggtcga
     2161 tcgccgcgag cgacgcctcg acgagcgccg tcatcgcggc gacgagcggc tctccgtctc
     2221 ccaggaacgt gaggacggag cggttgtggt cggagtccga cgtccgatcg aggagcacga
     2281 cgccgggcgc cgctttcacg gccgccgcgg cgatggccgc gacgacctcg tcgcgccgcc
     2341 cttcgctgac gttcgggatg cattcgatga gccgtgccat gtcgcctccc gcgaagccgc
     2401 gatcgtaccc gacgcgtcgg cgggaggttc ttgctagcat tcgccgaatg gcttcgtacg
     2461 cgggcagcgc cgctctctcg gcggaggcac gggaaaaggt cctccagacc ttccggcaca
     2521 ctctttctct cgcccagtcc ggcaaggccg acgaggccct gctgggctgc gacttcatcc
     2581 tcaagatgga cggccgcttc gccccggccc gggcgcttct cgacgcgctc cgcaccgcgg
     2641 cgcccggcgc ccccgtggac gtgaatcgat tctcggagtt tctcggattc gagagcgcgg
     2701 tcgaggagct gccgggctcg gccgagctgc cggccgtccc gaaatcggcg cccgcgccgc
     2761 cccttcgcga cgcgttcccc gggccggccc cgtcggctcc ccaggccggc atggacgacc
     2821 tcgtcttcgg cgacgagccc gcgcacgcgc cccaggccgc ggccgctcc
//
LOCUS       DAOMFY010000389         2847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_15454_length_2847_cov_7.234599, whole genome shotgun sequence.
ACCESSION   DAOMFY010000389 DAOMFY010000000
VERSION     DAOMFY010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15454_length_2847_cov_7.234599"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..827
                     /locus_tag="PLB01_19030"
     CDS             204..827
                     /locus_tag="PLB01_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQQ79449.1"
                     /translation="MNEERDVDSVLRESEAFFGGRGAVHRTLRSLAARLDAEGIPYAI
                     LGGMALNIHGFVRETVDVDVLMSRESLDRFRVRLVGRGYAPAFPGALKSFRDVETGVR
                     IDVIAAGDFPGDGRPKGVAFPEPAGVAVEKGGLRVVPLETLLELKLASGLSAEHRRLV
                     DLADVQRTIEALKLPKELGDRLHPSVRAEYGRLWALAQRVGEGPVER"
     gene            870..1604
                     /locus_tag="PLB01_19035"
     CDS             870..1604
                     /locus_tag="PLB01_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ79450.1"
                     /translation="MSASPVVLSAEGLAKRYGDREVVTDASFELHRGEVFGFLGPNGA
                     GKTTTIRMLVGLVKPTRGRVVIDGHDLEREFEAAMRKVGCIVETPDLYKFLTGRENLE
                     HFARMIGNGAAGRIDELARLVHMDERLDGLVRTFSLGMRQRVGIAQALLSDPSVLILD
                     EPTNGLDPAGIREMRELVRRLAGERGLAVFVSSHLLSEVEKMVDRIAIVHRGRTLAKG
                     TLSDLTDGGKRGLEDLFLELTGGETV"
     gene            1619..2614
                     /locus_tag="PLB01_19040"
     CDS             1619..2614
                     /locus_tag="PLB01_19040"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ79451.1"
                     /translation="MFGLIENECLKILRRKRFRLILVVLAALLCLVVFAQNRQQQRQR
                     LEHPERDWRASVEKRAGDLERQMNQRRGIPDQWKRWMRYESGRLRYHLSKNLDPNLLT
                     GPMFTRTFAGVGSVLLIPLLIAVFGADLVSSEFAEGTVTLLLTRPVARWKVLLSKLIA
                     MVLFTTLTLLAAFILAWGISGVAFGWSGWGAPMLTGFRLGPGAAGGFDLSAVRILPLW
                     QDAFAAWGLAWLSALAVGCVTLLLSVLFRSAAAAMGTMMATLVAGTILSRIASDWDGA
                     KWFFVTNLPLPEFYAGLPAPFEGMTLSFSAWVLAGWAVAATAAAFAVFTRRDVTA"
     gene            2646..>2847
                     /locus_tag="PLB01_19045"
     CDS             2646..>2847
                     /locus_tag="PLB01_19045"
                     /inference="COORDINATES: protein motif:HMM:NF027404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79452.1"
                     /translation="MSDSSSALVLNAMLEEFGDNAPFAQELYALYRLDPALVDEDWRR
                     AFRALEDRVPFAMSVPVRAIAPE"
BASE COUNT          383 a          960 c         1026 g          478 t
ORIGIN      
        1 ggagtgggga gggcgagcgg gccttcgccc acgcgctcgc cgaggcggcc ggacaggccc
       61 gcggcagcgc ctccggccag cacggcggcg gtcaggagcc cgagcgcgga gggggcgaac
      121 cagaggagca ggcggcgggt gcggagcgtc acggcggcgt attgtgccgg aagtccgtgc
      181 ggttccgggt ctaagatcgt ttcgtgaacg aagagcgcga cgtcgactcg gttctgcgcg
      241 agtccgaggc cttctttggg ggacgcgggg ccgtgcacag gacgcttcgg tcgctcgcgg
      301 cgcggctcga cgccgagggc atcccgtacg cgatcctcgg cgggatggcg ctgaacattc
      361 acgggttcgt ccgggagacc gtggacgtgg acgttctcat gtcgcgggaa tccctcgacc
      421 ggttccgcgt gcggctcgtc ggacgcggct acgctcccgc gttccccggc gccctgaaga
      481 gcttcaggga cgtcgagacg ggggtgcgca tcgacgtcat cgcggccggg gattttccgg
      541 gcgacggcag gccgaagggc gtcgcgtttc ccgagccggc gggtgtcgcg gtcgagaagg
      601 gcgggctccg cgtcgtccct ctggagacgc tcctcgagct gaagctcgcc tccggccttt
      661 cggccgagca tcgccggctc gtcgacctcg ccgacgtcca gcggacgatc gaggcgctga
      721 agcttccgaa ggagctcggc gaccggctcc accccagcgt ccgggccgag tacggccgac
      781 tgtgggcact ggcccaacgg gtcggcgaag ggccggtcga gcgttgacga gagaaaccgc
      841 cgcttgaccg atggcgtaaa acgtctgccg tgagcgcctc gcccgtcgtc ctgtccgccg
      901 agggcctcgc gaagcgctac ggcgaccgcg aggtcgtgac ggacgcctcc ttcgagctgc
      961 accggggcga ggtgttcggc ttcctgggcc cgaacggcgc ggggaagacg acgacgatcc
     1021 ggatgctcgt cgggctcgtc aagcccacgc gcggccgcgt cgtcatcgac gggcacgacc
     1081 tcgaacggga attcgaggcc gcgatgcgca aggtgggctg catcgtcgag acgcccgacc
     1141 tctacaagtt cctcacgggc cgcgagaatc tcgagcactt cgcccggatg atcgggaacg
     1201 gagcggcggg ccggatcgac gagctcgcgc gcctcgtcca catggacgag cgcctcgacg
     1261 gcctcgtgcg cacgttctct ctcgggatgc gccagcgcgt cgggatcgcg caggcgctgc
     1321 tctcggaccc gtccgtcctc atcctcgacg agcccacgaa cggcctcgac cccgcgggca
     1381 tccgcgagat gcgcgagctc gtccgccggc tcgcgggcga gcgcggcctc gccgtcttcg
     1441 tgtcgagcca cctcctgtcg gaggtcgaga agatggtcga ccgcatcgcg atcgtccacc
     1501 gcggacgaac gctcgcgaag ggtacgctgt ccgacctgac ggacggcggc aagcgcggcc
     1561 tcgaggacct cttcctcgag ctgacgggcg gggagacggt gtagacgcga tgcgagtcat
     1621 gttcgggctc attgagaacg agtgtttgaa gattcttaga aggaagaggt ttcgcctcat
     1681 cttggtcgtt ctggccgcgc ttctgtgtct ggtcgttttc gcccagaacc gtcagcagca
     1741 gcgccagagg ttggagcatc cggagaggga ttggcgcgcg tcggtcgaga agcgcgcggg
     1801 cgacctcgag cgccagatga accagcgccg cgggatcccg gaccagtgga agcggtggat
     1861 gcgctacgag agcgggagac tccgctatca cctttccaag aatcttgatc cgaatcttct
     1921 gacggggccg atgttcacgc gcacgttcgc gggcgtcggg agcgtcctcc tcatcccgct
     1981 cctcatcgcg gtcttcggcg cggacctcgt ctcgtcggag ttcgccgaag gcaccgtcac
     2041 gctcctcctc acgcgccccg tcgcgcgctg gaaggtgctg ctgtcgaagc tgatcgcgat
     2101 ggtgcttttc acgacgctga cgctgctggc cgcgttcatc ctcgcgtggg ggatctcggg
     2161 cgtggcgttc ggatggagcg gctggggcgc gcccatgctc accggcttcc ggctcggccc
     2221 gggcgccgcg ggcgggttcg acctctcggc ggtgcggatc ctgccgctct ggcaggacgc
     2281 gttcgccgcg tggggcctcg cgtggctctc ggcgctcgcc gtcggttgcg tcacgctgct
     2341 cctctcggtc ctgttccgct cggcggccgc cgcgatgggc acgatgatgg cgacgctcgt
     2401 ggccgggacg atcctctcgc gcatcgcgtc ggactgggac ggggccaagt ggttcttcgt
     2461 gacgaacctg ccgcttcccg agttctacgc gggtctgccg gctcctttcg aagggatgac
     2521 gctttcgttc tcggcgtggg ttctcgcggg ctgggccgtg gccgcgacgg ccgcggcgtt
     2581 cgcggtgttc acgcggcgcg acgtcacggc ctgaaccggc cccggcggct acaattgacc
     2641 ccttcatgag cgactcgtcc tccgccctcg ttctcaacgc catgctcgag gagttcggcg
     2701 acaacgcgcc gttcgcccag gagctctacg ccctctaccg cctcgacccg gccctcgtcg
     2761 acgaggactg gcgccgcgcg ttccgggcgc tggaggaccg cgttcctttc gcgatgagcg
     2821 tcccggtccg ggcgatcgcg cccgagc
//
LOCUS       DAOMFY010000390         2811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_15821_length_2811_cov_8.508345, whole genome shotgun sequence.
ACCESSION   DAOMFY010000390 DAOMFY010000000
VERSION     DAOMFY010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15821_length_2811_cov_8.508345"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..90
                     /locus_tag="PLB01_19050"
     tRNA            1..90
                     /locus_tag="PLB01_19050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Ser,seq:gga)
     gene            185..643
                     /locus_tag="PLB01_19055"
     CDS             185..643
                     /locus_tag="PLB01_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79453.1"
                     /translation="MKRSPSAALLAVFAAVLAAPAFAAGPLLEKEVDVPRATRVDVGL
                     VWEKCTLVDVESHNGPDDKMVEAAKAHDPKDLTFLLLRFRYANSDWIDHRVRLTAVLL
                     DGEGNVVGDASRTATMDKGQKDDTISFPMKIKTVDWPVAKKLRVTASFLK"
     gene            697..786
                     /locus_tag="PLB01_19060"
     tRNA            697..786
                     /locus_tag="PLB01_19060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:Ser,seq:cga)
     gene            complement(792..1526)
                     /locus_tag="PLB01_19065"
     CDS             complement(792..1526)
                     /locus_tag="PLB01_19065"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQQ79454.1"
                     /translation="MGPFASNYLSILGTSALLTVLFFILERARPAEADQPFRTWLRNV
                     AYLPFILAWILALQLLYTPVAAALLRWTGGGVLVRFVTPPRTVLGHVLFALGFALAWD
                     VWQYWVHRWQHASPLLWGTHALHHAEEALNASSQARHNPTNYVLFLVLYTPLLLLFGG
                     LGPHPVAAFVMFRLWGFVNHANVRVAFGPATPLLAGPQWHRIHHSTRPEHARRNFAAF
                     FPFLDALFGTYYAPRAGEYPPTGRPG"
     gene            complement(1538..2716)
                     /locus_tag="PLB01_19070"
     CDS             complement(1538..2716)
                     /locus_tag="PLB01_19070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQQ79455.1"
                     /translation="MSAPDVGDDERRLVDEVLRSGRLSMGPMLARFEEEFAAHAGVPH
                     AVGVSSGTAGLHLGMIAAGVGDGDLVVTSPFSFVASANAILYERGIPVFVDIDPETLA
                     LDAGLAAEAVESLARRLPDRGRWLPRGARVSPGTLRAVVPVHVFGRPAEIDGLAAAAR
                     GYGVALVEDACEAVGARTGGRPAGTAGDAGVFAFYPNKQMTTGEGGMLVTAHATWDEL
                     FRSLRNQGRDGDARWLHHVRLGFNYRLDEMSAAVGLAQLRRLDALLQGRRAVASAYDV
                     RLKSVAGVSPLSAPGPQAEKSWFVYVVRLDPAIDRTRLMERLEARGVPTRPYFSPIHL
                     QPFYRDRFGFEPGDFPHAEEAARRLLALPFHAGLAEDDLDYVCGCLADESARARDSAA
                     "
BASE COUNT          471 a          923 c         1033 g          384 t
ORIGIN      
        1 ggacaggtgt ccgagtggtt taaggagcac ggttggaagc cgtgtgtagc tgaaaggtta
       61 ccgtgggttc gaatcccacc ctgtccgcca gttttttcgt ccttcgaggt cagctccggc
      121 acacggtatg cactcccgtg cgggggtccg tccgttccgg cacctccgaa aggagacctc
      181 ttcgatgaag agatcgccct ccgccgccct tctcgccgtc ttcgccgcgg ttctcgccgc
      241 cccggccttc gccgcggggc ccctcctcga aaaggaggtc gacgtcccgc gcgcgacccg
      301 tgtcgacgtc ggcctcgtct gggagaagtg cacgctggtg gacgtggagt cgcacaacgg
      361 gccggacgac aagatggtcg aggccgcgaa ggctcacgac cccaaggacc tcacgttcct
      421 gctcctgcgt ttccgctacg cgaactccga ctggatcgac caccgcgtgc gcctcacggc
      481 ggtcctcctc gacggagaag gcaacgtcgt cggcgacgcg agccgtacgg cgacgatgga
      541 caagggccag aaggacgaca cgatcagctt cccgatgaag atcaagaccg tcgactggcc
      601 ggtcgcgaag aagctccgcg tgacggccag cttcctgaag tagcgcgatc ccgctctttc
      661 ccctatgatc cgcggctccg atcggccgga ccgtgcggtg aggtgctgga gtggttgatc
      721 aggacggtct cgaaaaccgt tttacccgaa agggtaacgt gggttcgaat cccaccctca
      781 ccgccagctg atcagcccgg acgcccggtc ggcgggtact ctcccgcgcg cggcgcgtaa
      841 tacgtgccga acagggcgtc caggaacggg aagaacgccg cgaagttccg acgggcgtgt
      901 tcgggccgcg tcgagtggtg gatgcggtgc cactgcggcc cggccaggag cggcgtggcc
      961 ggcccgaagg ccacccggac gttcgcgtga ttcacgaacc cccacaggcg gaacatgacg
     1021 aatgcggcga cggggtgcgg tccgaggcct ccgaagagga gaaggagcgg cgtgtacagg
     1081 acgaggaaca ggacgtagtt cgtcgggttg tgccgggcct gcgagctcgc gttcagcgcc
     1141 tcctcggcgt ggtgaagggc gtgcgtgccc cagaggagcg gcgacgcgtg ctgccacctg
     1201 tggacccagt attgccagac gtcccacgcg agagcgaagc cgagcgcgaa gagaacgtgg
     1261 cccaggaccg tgcgcggcgg cgtgacgaac cggacgagga cgccgccgcc cgtccagcgc
     1321 agaagagcgg ccgcgacagg cgtgtagagc agctgaagcg cgaggatcca cgcgaggatg
     1381 aacggaaggt acgcgacgtt gcggagccag gtgcggaacg gctggtccgc ctccgcgggc
     1441 cgcgcccgtt cgaggatgaa gaagaggacc gtgagcagag ccgacgtccc gaggatggac
     1501 aggtagttcg aagcgaaggg tcccatgggg cggcggctca ggccgcagag tcgcgggcgc
     1561 gcgcggactc gtccgcgagg cacccgcaca cgtaatccag gtcgtcctcg gcgaggcccg
     1621 cgtggaaagg cagggcgagg agccgccgcg cggcttcctc cgcgtgcggg aagtcgccgg
     1681 gctcgaagcc gaaccggtcc cggtagaacg gctggaggtg gatcggagag aaataaggcc
     1741 gcgtcggaac gccgcgcgcc tcgagccgct ccatgagacg cgtccggtcg attgccggat
     1801 cgagccgcac gacgtagacg aaccagctct tctcggcctg cggtcccggt gcggagaggg
     1861 gcgagacccc ggcgacggac ttcagccgca cgtcgtacgc cgacgcgacg gcgcggcggc
     1921 cctggaggag ggcgtcgagg cgccggagct gcgcgaggcc gaccgcagcg ctcatctcgt
     1981 ccaggcggta gttgaagccg agccggacgt gatggagcca acgtgcgtcg ccgtcgcggc
     2041 cctggttgcg caggctgcgg aagagctcgt cccacgtcgc gtgcgccgtc acgagcatgc
     2101 cgccttcgcc ggtcgtcatc tgcttgttcg ggtagaacgc gaagactccg gcgtcgcccg
     2161 cggttcccgc ggggcgtccg cccgttcggg cgccgacggc ctcgcaggcg tcctcgacga
     2221 gcgcgacgcc gtatccgcgt gcggcggccg ccagcccgtc gatctccgcg gggcggccga
     2281 acacgtgaac gggtacgacg gcgcggagag ttccggggct cacccgcgcg ccgcggggca
     2341 gccagcggcc gcggtcgggg aggcgccgcg cgagcgactc gaccgcctcg gcggcgagac
     2401 cggcgtccag tgcgagcgtt tcggggtcga tgtcgacgaa gacggggatc ccgcgctcgt
     2461 agaggatcgc gttggcggac gcgacgaacg agaacgggga cgttacgacg aggtccccgt
     2521 cgcccacgcc cgccgcgatc atcccgagat ggagcccggc ggtcccgctc gagacgccga
     2581 ccgcatgcgg cacgccggcg tgcgcggcga actcctcctc gaatcgcgcg agcatcgggc
     2641 ccatggagag gcggccggag cgaaggactt cgtcgacgag gcggcgttcg tcgtctccga
     2701 cgtcgggagc cgacatcgag acgggggggc gaatcggaac cgtgcgcacg ggtgcgcgga
     2761 cgatacaccg gggaggcccg tctagaatcc cctgcttccc atgcagatcg t
//
LOCUS       DAOMFY010000391         2807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_15856_length_2807_cov_7.581395, whole genome shotgun sequence.
ACCESSION   DAOMFY010000391 DAOMFY010000000
VERSION     DAOMFY010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15856_length_2807_cov_7.581395"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="PLB01_19075"
     CDS             <1..1857
                     /locus_tag="PLB01_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79456.1"
                     /translation="ATCLAKDPEDRIQTAHDVRLQLQWVGEGGSQAGLPAPVAHRRKS
                     RERLAWSLAAAAAVAAAVLAVGFVKRAPTRPKLVRFEIANPEGITTIDAPRLSPDGRT
                     LAYNATDASGKSRIWIRPLNSLTAQPLAGTEDTTRPFWSPDSRFLGFFAEGKLKKVEV
                     SGGPPQKICDAPGGADGSWSPEGVILFDGTGNQPINRVSAAGGTPVVAVKPDASRKEA
                     QVGWPEFLPDGKHFLYMAIGQKAEESAYRIGTLDSAETKLLAPAQTLVTYAPPGYLLF
                     VRDKTLVAQPFDAKALKTTGEPIPLAEHVGTDNVGLALFSVSRDGTLAYRTGERGDRL
                     LWVDRSGKEGEVVGDPGDYHNPSFSPGSDRLAFDLADPRSSKTDVWIRDLKRGVSSRF
                     TFGTDGAFGPLWSPDGRRMVYTVGDDLFEKAVGGQGAETPLLKSDETKVATDWSRDGR
                     TLAFHSLGKETGWDIWVIPLSGDRKPVPFLKTPFAELNGVFSPDGRFLAYQSSESGRQ
                     EIYVQAFPGPGGKWQISTSGGREPQWRADGKELYYRAFDQKLMAVDIQTGNDVTAGTP
                     HVLFQGRFDINVARNRFLPTADGKRFLTVAPLGRDSMSPTTIVLNWYAGLEK"
     gene            1859..>2807
                     /locus_tag="PLB01_19080"
     CDS             1859..>2807
                     /locus_tag="PLB01_19080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79457.1"
                     /translation="MTLAAGSRLGPYEILSPLGAGGMGEVYRAKDTRVDRVVALKVLP
                     EDFLEGEENRSRFEREAKLLASLNHPGIATLYSFEEIPGSSSPPSSPRSVLTMELIEG
                     ETLASRLAKGALPLQQVISAGVQIADALEAAHRQGIVHRDLKPGNVMLTKGGVKLLDF
                     GLAKTLAPQSQPSGLTSMPTMASPQHLTQKGTILGTFQYMAPEQLEGGDADARSDIFS
                     FGAVLFEMATGKKAFDGKSQASLIGSILKDSPPPASSISPMTPPALDRVIATCLAKDP
                     EDRLQTAHDVKLQLQWVGEGGSQAGLPAPVAARRKNREKL"
BASE COUNT          466 a          985 c          951 g          405 t
ORIGIN      
        1 gcgacgtgcc tcgcgaagga ccccgaggac cggatccaga cggcgcacga cgtgaggctc
       61 cagctccaat gggtggggga gggcggctcg caggccgggc tgcccgcccc ggtcgcccac
      121 cgccgaaaga gtcgcgagcg cctcgcgtgg agtcttgccg cggcggccgc cgtggcggcg
      181 gcggttctcg ccgtgggttt cgtcaagcgc gctcccacgc gcccgaagct cgtccggttc
      241 gagatcgcga accccgaggg catcacgacg atcgacgcgc cgcgcctgtc tccggacggc
      301 cggacgctcg cgtacaacgc gaccgacgcc tccggcaaga gccgcatctg gatccgcccg
      361 ctcaactcgc tcaccgcaca gcctctcgcc ggcaccgagg acacgacgcg gcccttctgg
      421 tcgcccgaca gccggttcct cgggttcttc gcggagggca agctcaagaa agtcgaggtt
      481 tcgggaggcc cgccgcagaa gatctgcgac gcgccgggag gcgccgacgg ctcctggagt
      541 cccgagggcg tcatcctctt cgacggcacg ggcaaccagc ccatcaaccg cgtatcggcg
      601 gcggggggca ctccagtcgt cgccgtcaag cccgacgcct cgcgcaagga ggcacaggtc
      661 ggctggcccg agttcctccc ggacgggaag cacttcctct acatggcgat cgggcagaag
      721 gccgaggaga gcgcataccg gatcgggacg ctcgactctg cggagacgaa gctcctcgcg
      781 cccgcgcaga cgctcgtgac gtacgcaccg cccggctatc tcctcttcgt gcgggacaag
      841 acgctcgtcg cccagccgtt cgacgcgaag gccctgaaga cgaccggcga gccgattccg
      901 ctcgccgagc acgtcggcac ggacaatgtc gggctcgcgc tcttctcggt ctcgcgcgac
      961 gggacgctcg cgtacaggac gggagagcgt ggcgacaggc tcctctgggt ggaccgctcc
     1021 ggcaaggagg gcgaggtcgt cggcgacccg ggcgactacc acaacccttc gttttctccg
     1081 ggcagcgacc ggctcgcgtt cgacctcgcc gatccgcgga gctcgaagac cgacgtctgg
     1141 atccgcgacc tcaagcgcgg cgtcagctcc cggttcacgt tcgggaccga cggcgcgttc
     1201 ggtcccctct ggtctcccga cggacggcgc atggtgtaca cggtgggcga cgacctgttc
     1261 gagaaggcgg tcggaggaca gggagcggag acgccgctcc tgaagtcgga cgagacgaag
     1321 gtcgccaccg actggagccg cgatggccga actctcgcct tccacagcct cgggaaggag
     1381 acggggtggg acatctgggt cattcccctg tccggcgacc ggaaaccggt cccgttcctg
     1441 aagacgccgt tcgcggaact caacggcgtg ttctcgccgg atggccgctt cctcgcgtat
     1501 cagtccagcg agtcgggacg ccaggagatc tacgtccagg cctttccggg cccggggggc
     1561 aagtggcaga tctcgacctc gggcggccgg gagccgcagt ggagagccga cggcaaggag
     1621 ctttactacc gggcctttga ccagaagctg atggcggtcg acatccagac gggaaacgac
     1681 gtcacggccg gcacgccgca cgttctcttc caggggcgct tcgacatcaa cgtcgcccgc
     1741 aaccgctttc tcccgaccgc cgacggcaag cgttttctca ccgtcgcccc gcttgggcgt
     1801 gactcgatgt ctccgacgac gatcgtcctg aactggtacg cggggctgga gaagtgacgt
     1861 gacgctcgca gcgggcagcc gcctcgggcc gtacgagatc ctctcgccgc tgggcgccgg
     1921 cgggatgggc gaggtctatc gggccaagga cacgcgcgtc gaccgggtgg tcgcactgaa
     1981 ggtattgccg gaagatttcc tggaaggcga agagaaccgg tcgcgcttcg aacgcgaggc
     2041 gaagctgctc gcgagcctga atcatccagg gatcgccacc ctctactcat tcgaagaaat
     2101 ccccggttct tcgtccccgc cctcttcgcc tcgaagcgtc ctgaccatgg agctcatcga
     2161 gggcgagacg ctcgcaagcc gcctcgcgaa gggcgcgctg ccgctccagc aggtgatttc
     2221 cgccggggtc cagatcgcgg acgccctcga ggcggcgcac cgccagggga tcgtccaccg
     2281 cgacctcaag ccgggcaacg tcatgctcac gaagggcggc gtgaagctgc tcgacttcgg
     2341 cctcgccaag acgctggccc cgcagtcgca gccctcgggc ctcacgtcga tgccgacgat
     2401 ggcgtcgccg cagcacctca cgcagaaggg gacgatcctc gggacgttcc agtacatggc
     2461 gcccgagcag ctcgagggcg gggacgcgga cgcgcggagc gacatcttct ccttcggggc
     2521 tgtcctcttc gaaatggcga ccgggaagaa ggcgttcgac ggcaagagcc aggcaagcct
     2581 gatcggctcg atcctgaagg acagcccgcc gcccgcttcg tccatctcgc cgatgacgcc
     2641 gccggccctc gaccgcgtca tcgcgacgtg cctcgcgaag gacccggagg atcgcctcca
     2701 gacggcgcac gacgtgaagc tccagctcca gtgggtcggc gaaggcggat ctcaggccgg
     2761 ccttcccgcg cccgtcgccg cccggcgcaa gaaccgcgag aagctcg
//
LOCUS       DAOMFY010000392         2753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_16413_length_2753_cov_5.962565, whole genome shotgun sequence.
ACCESSION   DAOMFY010000392 DAOMFY010000000
VERSION     DAOMFY010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16413_length_2753_cov_5.962565"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PLB01_19085"
     CDS             <1..1316
                     /locus_tag="PLB01_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79458.1"
                     /translation="TTAGFGAAGASSLSRTSLDVSGALGAGVRWGASGQYARLADLAG
                     EKTGDSARLALEVDAGASQSVHISTRRQNLSAEADPTRFAAHALDWSGAVSERGQAAL
                     SARVISQAHLLATGPAADLFARSSDAVDVAARYQTDLGAATFARVTVGFRSTSGDYAG
                     TVTPFEHETRAGGVAGLRLGEILVVEAGASGDSSFYGRGITPELMVALQTKDGWRIYG
                     FASRRFESAAETPAVRPGEAGADEADLTRRSRSLYRGGVRWQNRDGESFAVDVSRREI
                     NGTYRLLFDADFLDRLDSLYFFPNDVATDAALTVSARLGAGFDGRVSLRAGTLSGARD
                     GAIRSDEANWGVAQAALRVRATDTSVGIGYRQVAQSLQRGPTTLHNDLQAVDVTLAQG
                     LPLPILRAVGSDWRALFSVEFGKRREGEDEEKANRRLAGGLALSF"
     gene            1494..>2753
                     /locus_tag="PLB01_19090"
     CDS             1494..>2753
                     /locus_tag="PLB01_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79459.1"
                     /translation="MARVGLVSLLLAGLFPSALSLVRKAATFGRADVLVAPEGDGLRV
                     HRVGPSAVPSGLAPGDILLLVDGAGARKSLEPAKLLAERAADVVLLRDGVTRSLRTAP
                     VPAPWDVRYLFLFAVGGTFLASALAALRPARPSKEALLYAAFALSVGLVLTLTPAPPL
                     DGIFRIAVLAEDAARALFPAFLLALVLTFPRRARHVPAGLAFVPAAALLLATARVYFG
                     PGANAAADVAALDRAQIAWMAAGATLAAVRLVVVARRPTDLLTEKQVRFLLLGTAVGI
                     LPLVVLNFVPRLLGGSIPILSTLALLPLALVPVAFLAALTRYRLWDVEALGREAASVT
                     GALLVGAGFFALAEMLLAHPLALAIPHARGALQTSAGLLMALSFVPVRRGLSAAFRRF
                     QVRDDLGDRDGLLALLRELPSPRPLAEL"
BASE COUNT          288 a         1094 c          921 g          450 t
ORIGIN      
        1 cgacgacggc gggcttcggc gcggccggcg cctcgtcgct ctcgcggacc tcgctcgacg
       61 tctcgggagc gctcggcgcc ggcgtgcgat ggggcgcctc cggccagtac gcgcgcctcg
      121 cggacctcgc aggcgagaag acgggcgact ccgcgcggct cgcgctcgaa gtcgacgccg
      181 gagcctcgca gtcggttcac atctcgacgc gccgacagaa cctctcggcc gaggccgacc
      241 ccacccgctt cgcggcccac gcgctcgact ggagcggtgc cgtttccgag cgcgggcagg
      301 ccgctctctc ggcgcgcgtg atctctcagg cccatctcct cgcaaccggt cccgcggcgg
      361 acctcttcgc gcgctcgtcc gacgccgtcg acgtggccgc gcggtaccag acggacctcg
      421 gggccgccac gttcgcgcgc gtgaccgtcg gcttccgctc gacgtccggc gattacgccg
      481 gaaccgtgac gccgttcgag cacgagacac gcgcgggcgg cgtcgcgggc cttcgcctcg
      541 gcgagatcct cgtcgtcgag gccggggcgt cgggcgactc gtcgttctac ggccgcggca
      601 tcacgccgga gctcatggtc gctctgcaaa cgaaggacgg ctggcgcatc tacgggttcg
      661 cgtcccgccg cttcgagagc gccgccgaga cgcccgccgt gcggcccggc gaggccggcg
      721 ccgacgaagc cgacctgacg cgccgctcgc gctcgctcta ccgcggcggc gtgcggtggc
      781 agaaccgcga cggcgagagc ttcgccgtcg acgtctcgcg ccgcgagatc aacgggacgt
      841 accggctcct gttcgacgcg gacttcctcg accgcctcga ctcgctctat ttcttcccga
      901 acgacgtcgc gaccgacgcg gcgctcaccg tctcggcccg cctcggcgcc ggcttcgacg
      961 ggcgcgtgtc gctccgggcc ggcacgctgt cgggcgcgcg cgacggcgcc attcgctcgg
     1021 acgaagcgaa ctggggcgtc gcgcaggcgg ccctccgcgt acgggcgacg gacacgtccg
     1081 tcggcatcgg ataccggcag gtcgcccagt ccctccagcg cggccccacg acgcttcaca
     1141 acgacttgca ggccgtggac gtcacgctcg cccagggact gccgcttccc attctccggg
     1201 cggtcgggag cgactggcgg gctctcttct cggtcgagtt cggaaagcgc cgcgaaggcg
     1261 aggacgaaga gaaggcgaat cgccgcctcg cgggcggcct cgccctgtcg ttctgactct
     1321 ccaaactccc atagttcact tcaagttctt gattcgagat gccgtctcgg cgcggccgct
     1381 gctccctaac tgaccgcgaa tcagttcttt ttcgaaattc aaagtcccgg atcgttggca
     1441 ttcgacttgc tccgcgcgaa cgcatggaga cggccggctc gacgggtcgc gccttggcgc
     1501 gtgtggggct cgtgtccctc ctgctcgcgg gtctgttccc ctcggcgctc tccctcgtcc
     1561 gcaaggcggc cacgttcgga agggccgacg ttctcgtggc gcccgaaggc gacggtctgc
     1621 gagtgcaccg cgtgggcccg tccgccgtcc cgtcgggcct tgcgcccggc gacatcctgc
     1681 tcctcgtcga cggtgccggc gcccgcaagt cgctcgagcc ggcaaagctg ctggccgagc
     1741 gcgcggccga cgtcgttctc ctccgcgacg gcgtcacgcg cagcctcagg acggcgcccg
     1801 tgcccgcccc gtgggacgtc cggtacctgt tcctcttcgc ggtcgggggc acgttcctcg
     1861 cctcggcgct cgcggcgctg cggcccgccc ggccctcgaa ggaggcgctg ctctacgcgg
     1921 cgttcgctct ctcggtcggg ctcgtcctca cgctgacgcc cgcgcctccc ctcgacggga
     1981 tcttccgcat cgcggtcctc gccgaggacg ccgcgcgcgc cctgttcccg gcgttcctgc
     2041 tcgcgctcgt cctcaccttc ccgcgccgcg cgcgccacgt ccccgccgga ctcgcgttcg
     2101 tgccggcggc ggcgctcctc ctcgcgacgg cccgggtcta cttcggcccc ggcgcgaacg
     2161 ccgccgccga cgtcgccgcg ctcgaccggg cccagatcgc ctggatggcg gcgggcgcca
     2221 cgctcgcggc cgtccgcctc gtcgtcgtcg cgcggcgccc gaccgatctc ctgaccgaaa
     2281 agcaggtgcg cttcctcctc ctcggaaccg cggtcgggat cctgcccctc gtcgtcctga
     2341 acttcgtccc gcgcctgctc ggcgggtcca ttccgattct ctcgacgctc gcgctcctcc
     2401 cgctcgctct cgtgcccgtc gccttcctcg cggccctgac gcgctaccgg ctgtgggacg
     2461 tcgaggccct cggacgggag gcggcgtccg tgacgggcgc gctcctcgtc ggggccggct
     2521 tctttgcgct cgcggagatg ctcctcgcgc acccgctcgc cctcgcgatc cctcacgcgc
     2581 gcggcgcgct tcagacctcc gcgggcctcc tgatggcgct cagcttcgtg ccggtgaggc
     2641 gcggcctctc ggccgcgttc cgccgtttcc aggttcgcga cgacctcggc gaccgcgacg
     2701 ggctgctcgc tctcctgcgt gagctgccgt ctccgcggcc tctcgccgag ctc
//
LOCUS       DAOMFY010000393         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_16921_length_2707_cov_6.613876, whole genome shotgun sequence.
ACCESSION   DAOMFY010000393 DAOMFY010000000
VERSION     DAOMFY010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16921_length_2707_cov_6.613876"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2707)
                     /gene="gltB"
                     /locus_tag="PLB01_19095"
     CDS             complement(<1..>2707)
                     /gene="gltB"
                     /locus_tag="PLB01_19095"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HQQ79460.1"
                     /translation="DATWEAATGAEGLVAALDALAAGAEGAVRAGAEILVVTDKGMTR
                     ERAAIPALLALGSVREHLLRAGLRYRVGLVVEAGDARDVHHLAVLHGFGAEAVHPWLA
                     LASVRALAAKDGLGGDAAASFRAASEKGLRKILAKMGISALSSYCGGQVYDALGLAPA
                     VMEKCFRGTASPLGGLGFAEIAEDALAAHRAAWEGTALPDHGRVRYRKDAEEHAWAPP
                     VVVSLQKAAKGSEEAWADFRTKADARAPSVPRDLIGFAPAAPVGVEEVEAASEIAKRF
                     IVSAMSLGALSPEAHETLAIAMNRLGARSNSGEGGEDPATWTGSGAERKDNRIKQVAS
                     ARFGVTAEYLVRADELEIKVAQGAKPGEGGQLPAHKNSPLIARLRHAVPGIALISPPP
                     HHDIYSIEDLAQLIHDLKEVNPKARVGVKLVSEAGVGRVAAGVAKAFADYVLIAGHSG
                     GTGASPLSSIKHAGSPWELGLAETQQALVENGLRKRIEVRVDGGLRTAKDVVIAACLG
                     AEAFGFGTAPLVAAGCAMARQCHLNTCPTGVATQREDLRKKYRGTPEMVVAYFLHLAE
                     DVREVLASLGARTIDEIVGRADLLRRVERPESPRSRLLDLSLLVQRAGDETSPRRRMQ
                     NRNDRPRTGPQFPSLDSELAPHVAVSLGTGRAYSGTFEVRNHNLSVGTHLSGELVRTF
                     GSLPPGAVRLKFRGPAGQSFGAFAIRGVTLELEGEANDGVGKGLSGAEIVIRPYPRAG
                     YIARAREQALLGNAALYGATSGRLFAAGRAGDRFAVRNSGAAAVVEGVGAHGCEYMTG
                     GTVVVLGKTGANFGAGMTNGLAYVLDEDSLFSSRCNTELVSVDALTSEDDAALVPLLV
                     AHAERTGSAIARSILSGWPSARSVFRRVTPKPPIFPRAKCFIWSPA"
BASE COUNT          361 a         1020 c          974 g          352 t
ORIGIN      
        1 ctgccggtga ccagataaag cactttgcgc gcgggaagat cggcggcttc ggcgtcacgc
       61 ggcggaacac cgagcgggcc gacggccagc cggagaggat cgacctcgcg atcgcgctgc
      121 ccgtgcgctc cgcgtgggcg acgaggagcg ggacgagcgc cgcgtcgtcc tcggacgtca
      181 gcgcgtcgac cgacacgagc tccgtgttgc agcgcgacga gaagagggag tcctcgtcga
      241 gcacgtacgc gaggccgttc gtcatcccgg cgccgaagtt cgcgcccgtc ttcccgagga
      301 cgacgaccgt ccctcccgtc atgtactcgc agccgtgggc gccgacgcct tcgacgaccg
      361 cggcggcgcc cgagttgcgc accgcgaagc ggtcgcccgc ccggcccgcg gcgaagaggc
      421 gccccgacgt cgcaccgtag agcgcggcgt tcccgaggag cgcctgctcc cgcgcccgcg
      481 cgatgtaacc cgctcgcgga tacggccgga tcacgatctc ggcccctgaa aggcccttcc
      541 cgacgccgtc gttcgcctcg ccctcgagct cgagcgtcac gccgcggatc gcgaacgccc
      601 cgaagctctg ccccgccggg ccgcggaact tcaggcgcac ggcgccgggc ggcagcgagc
      661 cgaatgtccg gacgagctcg ccggacaggt gcgtgccgac cgagaggttg tggttgcgca
      721 cctcgaacgt cccggagtac gcccggcccg ttccgaggct cacggcgacg tgcggcgcga
      781 gctcgctgtc gagagacgga aactgcggcc ccgtgcgcgg ccggtcgttg cggttctgca
      841 tccgccggcg cggggacgtc tcgtcgcccg cgcgctgcac gaggagcgac aggtcgagga
      901 gccgcgaccg gggcgactcc gggcgttcga cgcgccggag gaggtctgcg cggcccacga
      961 tctcgtcgat cgtgcgcgcg ccgagggacg cgagaacctc gcgcacgtcc tcggcgaggt
     1021 gcagaaagta cgccacgacc atctcgggcg tcccgcggta cttcttccgc aggtcctcgc
     1081 gctgcgtcgc gacgcccgtc gggcaggtgt tgaggtggca ttgccgcgcc atcgcgcagc
     1141 cggccgcgac gagcggcgcg gtcccgaagc cgaacgcctc ggcgccgagg caggccgcga
     1201 tcacgacgtc cttcgccgtc cgcaggccgc cgtccacacg cacctcgatc cgcttcctca
     1261 ggccgttctc gacgagggcc tgctgcgtct cggcgagacc gagctcccac ggcgaacccg
     1321 cgtgcttgat ggacgagagc ggcgaggccc ccgttccgcc cgagtggccc gcgatgagga
     1381 cgtagtcggc gaacgccttt gcgacgcccg cggccacgcg gccgacgccg gcctccgaca
     1441 cgagcttcac gcccacccgc gccttcgggt tgacctcctt caggtcgtgg atgagctgcg
     1501 cgaggtcctc gatcgagtag atgtcgtggt gcggcggcgg cgagatgagc gcgatgcccg
     1561 gcaccgcgtg cctcaggcgc gcgatgagag gcgagttctt gtgcgccggg agctggccgc
     1621 cctcgccggg cttggcgccc tgcgcgacct tgatctccag ctcgtcggcc ctcacgaggt
     1681 actcggcggt cacgccgaaa cgcgccgacg cgacctgctt gatccggttg tccttccgct
     1741 cggcgcccga gccggtccac gtggcggggt cctcgccgcc ctcgccggag ttcgaacgcg
     1801 ctccgagccg gttcatcgcg atcgcgagcg tctcgtgcgc ctcgggcgag agcgcgccga
     1861 gcgacatcgc cgagacgatg aagcgcttcg cgatctcgga cgcggcctcg acttcctcga
     1921 cgccgacggg tgcggccgga gcgaagccga tcaggtcgcg cgggaccgac ggcgcgcgcg
     1981 cgtccgcctt cgtccggaag tccgcccacg cctcctccga gcctttcgcg gccttctgga
     2041 gcgacacgac gaccggcggc gcccacgcgt gctcctcggc gtccttgcgg tagcgcacgc
     2101 ggccgtggtc cggcagcgcc gtgccctccc acgcggcccg gtgagcggcg agcgcgtcct
     2161 cggcaatctc ggcgaagccg aggccgccga gaggcgaggc cgttccacgg aagcacttct
     2221 ccatgaccgc aggcgcgagg ccgagcgcgt cgtagacctg gccgccgcag taggacgaca
     2281 gggccgagat ccccatcttc gcgaggatct tcctcaggcc cttctccgac gccgcgcgga
     2341 acgaggccgc cgcgtcgccg ccgaggccgt ccttcgcggc gagcgcgcgg acggacgcga
     2401 gcgcgagcca cggatgcacc gcttcggcgc cgaacccgtg caggacggcg aggtgatgga
     2461 cgtcgcgggc gtcgcccgcc tcgacgacga ggccgacgcg gtagcggagc cccgcgcgga
     2521 gcaggtgctc gcgcaccgag ccgagggcga ggagcgccgg gatcgccgca cgctccctcg
     2581 tcatcccctt gtccgtgacg acgaggatct cggcgcccgc gcgcacggcg ccctccgcgc
     2641 ccgccgcgag ggcgtcgagc gcggcgacga ggccctcggc gccggtggcg gcctcccacg
     2701 tcgcgtc
//
LOCUS       DAOMFY010000394         2688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_17161_length_2688_cov_5.690087, whole genome shotgun sequence.
ACCESSION   DAOMFY010000394 DAOMFY010000000
VERSION     DAOMFY010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17161_length_2688_cov_5.690087"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLB01_19100"
     CDS             complement(<1..209)
                     /locus_tag="PLB01_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQQ79461.1"
                     /translation="MTSDELPDERALVPYEASRFDARRVLVLAPHPDDEVFGCGGALA
                     DLASRGATIDVLLVTDGAASTSDEAG"
     gene            232..1281
                     /locus_tag="PLB01_19105"
     CDS             232..1281
                     /locus_tag="PLB01_19105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ79462.1"
                     /translation="MDAAQPSPRVSVILRTKDRPDLLAEALASLRAQTFADFETVLVN
                     DGAPLPGELLAPPPGRALRVVAPPPPGGRTRALNAGLAAARGSWIAYLDDDDLYLPGH
                     LAALDAARAAGARAAHTSVRQVRQEKGPDGAWREAGTITTYDRPLDAGRLLLKNETPL
                     IGVGHERTLAEAAGGFDPAFDLFEDWDFLIRLARLAPVVHVPEVTAVYRVRAGSNVTE
                     MAPWGSPAAEEARRRLYAKHATAVTPEAMIAFVDGAVGPLDAALGRERFLVGEYARVE
                     AEKRVLEAALAAAHEDARQFREESATREQTLAVQNSHLRAEVAKRDATLEEIYRSRVW
                     RFFTPWWRLKERLRK"
     gene            1278..2447
                     /locus_tag="PLB01_19110"
     CDS             1278..2447
                     /locus_tag="PLB01_19110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ79463.1"
                     /translation="MTAPSPGVRCDLVIPVHNALRSTRACLSSVKRFAPGWARVVVVN
                     DASDEATTAFLRAQDGIVLLENERNLGFVESANRGLLFSDAPWVCLLNSDTLLTEGAL
                     ERMVAGCEKDPAIGLATPLSNGAVNLSVVLPEGEDVFSFGRRVGRTSPARYPDAVTVV
                     GFCLLVKRDLIASLGVFDTIFGRGYCEETDLHYRARAAGRRCVVADDVWIYHRHGGSF
                     TDGNERTARNMEILMSRWREAHEREIREFNRRNDLGAVRDAATFEWILPDDRPPVPHD
                     LLVLVPPGPLAPATADRLELANELVLAGFRAGAVALDGAEPEKEMELWFRPYRRTAVT
                     LRTAPPAARAWAAADPETAASRPGEISSSERGAREGLAHSPLGSLRRRLASLFTF"
     gene            complement(2472..>2688)
                     /locus_tag="PLB01_19115"
     CDS             complement(2472..>2688)
                     /locus_tag="PLB01_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79464.1"
                     /translation="APAGNGALSLPRPAAVALSPLADFSSFAFVPRAQAGAWRPPEDV
                     VAGFGGAPWYARGVDLATLRVVAECAL"
BASE COUNT          370 a         1002 c          962 g          354 t
ORIGIN      
        1 cccgcctcgt ccgaagtgga cgcggcgccg tccgtcacga ggaggacgtc gatcgtcgcg
       61 ccgcgcgacg cgaggtccgc gagggcgccc ccgcagccga agacttcgtc gtcggggtgc
      121 ggcgcgagga cgaggacgcg gcgcgcgtcg aaacgcgacg cctcgtacgg gacgagcgcg
      181 cgctcgtccg gaagctcgtc ggaggtcacc ctgggagctt aaactccctc cgtggacgcc
      241 gcacagccca gtccgcgggt gtccgtcatc ctgcgcacga aggaccggcc ggacctcctc
      301 gccgaggcgc tcgcgagcct gcgcgcgcag acattcgcgg atttcgagac ggtcctcgtg
      361 aacgacggcg cgcccctccc cggggaactg ctcgcccccc ctccgggccg ggcgttgcgc
      421 gtcgtcgcgc ctcccccccc ggggggccgc acgcgggcgc tcaacgcggg gctcgccgcg
      481 gcccgcggaa gctggatcgc gtacctcgac gacgacgacc tctaccttcc cggccacctc
      541 gccgcgctcg acgccgcgcg tgctgcgggc gcgcgtgcgg cgcatacgtc cgtccgtcag
      601 gtccgtcagg agaagggacc cgacggagcg tggcgcgaag ccggtacgat cacgacgtac
      661 gaccgtccgc tcgacgcggg ccgcctcctc ctcaagaacg agacgccgct catcggcgtg
      721 ggccacgagc ggacgctcgc ggaagcggcc ggggggttcg atccggcgtt cgacctcttc
      781 gaggactggg acttcctgat ccgcctcgcg cgccttgcgc ccgtcgtcca cgtgcccgag
      841 gtgacggccg tctaccgcgt ccgcgccggc tccaacgtca cggagatggc gccgtggggc
      901 tctcccgccg ccgaggaggc gcgccgccgc ctgtacgcca agcatgcgac cgccgtgact
      961 cccgaggcga tgatcgcctt cgtggacggg gcggtcggac ccctcgacgc cgcgctcggc
     1021 cgcgagagat tcctcgtcgg cgaatacgcg cgcgtggagg ccgagaagag agtcttggag
     1081 gccgcgctcg cggcggccca cgaggacgcc cggcagttcc gcgaggagtc cgcgacgcgc
     1141 gagcagacac tcgcggtcca gaacagccac ctccgcgccg aggtcgccaa gcgcgacgcc
     1201 acgctcgagg agatctaccg gtcgcgcgtg tggcgcttct tcacgccgtg gtggcgcctg
     1261 aaggagcgcc tgcggaaatg acggccccct cccccggggt ccgctgcgac ctcgtgattc
     1321 ccgtccacaa cgcgctgcgc tcgacgcgcg cgtgcctctc gagcgtgaag cggtttgcgc
     1381 ccggctgggc gcgcgtcgtc gtcgtgaacg acgcgagcga cgaggcgacg acggccttcc
     1441 tgcgggcgca ggacggcatc gtcctcctcg agaacgagcg gaatctcggg ttcgtggagt
     1501 ccgcgaaccg cggcctcctc ttctcggacg cgccctgggt ctgcctcctg aacagcgaca
     1561 cgctgctcac ggaaggggcg ctcgagcgga tggtcgcggg ctgcgagaag gaccccgcga
     1621 tcggcctcgc gacgccgctc tcgaacggcg ccgtgaacct ctccgtcgtg ctccccgagg
     1681 gcgaggacgt gttctcgttc ggccggcgcg tgggccggac gagcccggcg cgctatcccg
     1741 acgccgtcac ggtcgtgggc ttctgcctcc tcgtaaagcg ggacctcatc gcgtcgctcg
     1801 gcgtcttcga cacgatcttc gggcgcggct actgcgagga gacggacctc cactaccgtg
     1861 cgcgcgcggc cgggcggcgc tgcgtcgtcg cggacgacgt ctggatctac caccggcacg
     1921 gcgggagctt cacggacggc aacgagcgca cggcgcgaaa catggagatc ctcatgagcc
     1981 ggtggcggga ggcgcacgag agggagatcc gggagttcaa ccgccggaac gacctcggcg
     2041 ccgtgcgcga cgccgcgacg ttcgagtgga tcctcccgga cgaccggccc ccggtcccgc
     2101 acgatctgct cgtcctcgtc ccgccgggcc cgctcgcgcc cgcgaccgcg gaccgcctcg
     2161 agctcgcgaa cgagctcgtg ctcgcgggct tccgcgcggg agccgtggcg ctggacggcg
     2221 ccgagccgga gaaggagatg gaactgtggt tccggccgta tcgccgcacg gcagtgacgc
     2281 tgcgcaccgc gccgcccgcg gcgcgggcgt gggccgccgc ggatccggag acggccgctt
     2341 cgcggcccgg agagatttct tcgagtgaaa gaggagcgcg cgaagggctc gcccacagtc
     2401 cgctcggctc gcttcgccgc cgcctcgcct ccctcttcac cttctaagaa atcttctctt
     2461 cctcgtctcc tctacagggc gcactccgcg acgacccgga gcgtggcgag gtcgactccc
     2521 cgcgcgtacc acggcgcgcc gccgaaaccc gcgacgacgt cctccggcgg acgccaggcg
     2581 cccgcctgcg cgcggggcac aaacgcaaag gacgagaaat ccgcgagcgg cgacagggcg
     2641 acggctgccg ggcgcgggag ggagagcgcg ccgttccccg cgggggcg
//
LOCUS       DAOMFY010000395         2674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_17315_length_2674_cov_5.252386, whole genome shotgun sequence.
ACCESSION   DAOMFY010000395 DAOMFY010000000
VERSION     DAOMFY010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17315_length_2674_cov_5.252386"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..815)
                     /locus_tag="PLB01_19120"
     CDS             complement(87..815)
                     /locus_tag="PLB01_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79465.1"
                     /translation="MEDAAPDEEAYATKVEEAFIAERGTPFLLSPKDWTLIRGWREGG
                     IPVDVVIRAVRDAFEKRRARGQAGKISSIAYCANSVEELWEMERRGLVGKTRETPAAG
                     ADAPAREESLALLSKSLRAMGELLATADGAEEFLRRVSGAEAPGIRKAVIGAASKIEA
                     LPRDLSFEESEGALSAIEVALVKSALRSLGPKQREFIEGRVAADLGDVAGVSPAVVER
                     MRRALTRREVRRLLGLPALTLLHG"
     gene            complement(817..1638)
                     /locus_tag="PLB01_19125"
     CDS             complement(817..1638)
                     /locus_tag="PLB01_19125"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQQ79466.1"
                     /translation="MSGGAPLPPAGSPLGALAPLGGRRFARIHNLILLAEVPSTNDFA
                     KLIVEHALAESEEIRLTVIAAGEQTAGRGRAGRTWVPLPGALALSVIVPWPEGPGRVR
                     LPIETGILLARGLSAAFGIDVRLKWPNDLLVDRRKLGGLLIEARSNDDGEGWAVIGIG
                     LNVRGTRADLDARGLPDATSLEASGVAPALLEGETPLDEVLAILDEAVGEPCADPLPE
                     AFAAVSAHAPGDLLTITDGDRSHKGAFAGVTSDGFLRLATESGDETLVSGDVVHF"
     gene            complement(1635..>2674)
                     /locus_tag="PLB01_19130"
     CDS             complement(1635..>2674)
                     /locus_tag="PLB01_19130"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQQ79467.1"
                     /translation="CDEFGADAVRFTLAVLSGTGRDLPFGKTRVAGYRAFATKVWNAT
                     RFALPLIAEGECADPQALDFRALSTVDRWILARLSDAAGRVNASLEAYRFDEAAQALY
                     QFFWSEFCDGYVEMIKPVLRGADVPEAEKAKTRGVLKRVLLDSLALLHPFMPFVSCEI
                     REALNGDGLQLTMAKFPEPRNEWKDDAAVEVVELLRAIVTRVRNLRAERGLPQTEALR
                     LGIELPPGPLSEEMQRRVPLLSHLARLSAVEISSSVNWPGAHRDVIADAGIVVDLPKQ
                     KISSEDAEKMRRDVEKLEAEAEKIRARLADESFLSRAPAAVVAKTKLQLEEISERIVR
                     LSGNLVGTQAP"
BASE COUNT          402 a          954 c          921 g          397 t
ORIGIN      
        1 tgacgacgcc gtccacgcgc gcgagggctt cgccgccggg cacgacgcgc tcgacgacgg
       61 cctcgagggc gtcgcccggg cggatctcag ccatggagga gcgtcagggc cggaagaccc
      121 agaagtcgcc gcacctcgcg gcgcgtgagc gcccgccgca tgcgctcgac gacggcgggc
      181 gacacgcccg cgacgtcgcc gaggtccgcg gccacgcggc cctcgatgaa ttctctttgc
      241 ttcggaccca gactgcgaag agccgacttc acgagggcca cctcgatcgc ggagagggcg
      301 ccttcggatt cttcgaatga aagatcgcgc ggcagggcct cgatcttcga ggcggctccg
      361 atcacagcct tccggatccc cggcgcttcc gccccactca cccttctgag gaattcttcc
      421 gcgccgtctg cggttgcgag aagttcgccc atcgcacgca ggcttttcga aagcaaagca
      481 agagattctt ctctcgcggg cgcatccgcg cccgcagcgg gagtctcgcg ggtcttgccc
      541 acgagaccgc gccgttccat ctcccacagc tcctcgaccg agttcgcgca gtacgcgatg
      601 gacgagatct tcccggcctg cccgcgcgcg cgccgtttct cgaacgcgtc gcgcacggcg
      661 cggatcacga cgtccaccgg gattccgccc tcgcgccagc cgcggatgag cgtccagtcc
      721 ttcggcgaca ggaggaacgg cgtgccgcgc tcggcaatga aagcctcctc caccttcgtg
      781 gcgtaagcct cctcgtcggg cgcggcgtct tccatgtcag aagtggacga cgtcccccga
      841 cacgagcgtc tcgtcgccgg actcggtcgc gaggcgcagg aaaccatcgg acgtgacgcc
      901 cgcgaacgcc cccttgtgcg agcggtcccc gtccgtgatc gtcaggagat cgcccggcgc
      961 gtgcgccgac accgccgcga acgcctcggg aagcgggtcg gcgcagggct cgccgacggc
     1021 ctcgtccagg atcgcgagca cctcgtcgag cggcgtctcg ccctcgagca gcgccggcgc
     1081 gacgccggac gcctcgagcg acgtggcgtc gggaaggccg cgggcgtcga ggtccgcgcg
     1141 cgtgccgcgg acgttcaacc cgatgccgat cacggcccat ccctcgccgt cgtcgttcga
     1201 tcgggcctcg atgaggaggc cgccgagctt ccggcggtcc acgaggaggt cgttcggcca
     1261 cttgagcctc acgtcgattc cgaaggcggc ggacaggccc cgcgcgagga ggattccggt
     1321 ctcgatcggg agccggacgc ggccgggtcc ctcgggccac gggacgatca ccgagagcgc
     1381 gagagcgccc gggagaggga cccacgtgcg ccccgcgcgg ccgcggccgg cggtctgctc
     1441 gcccgcggcg atcaccgtga gccggatctc ctcgctctcg gcgagggcgt gctcgacgat
     1501 gagcttcgcg aagtcgttcg tcgacggtac ctccgcgagg aggatcaggt tgtggatgcg
     1561 cgcaaagcgc ctcccgccga ggggcgcgag cgccccgagg ggcgagcccg cgggcgggag
     1621 gggcgcgccg ccgctcacgg ggcctgcgtc cccacgaggt tgcccgagag gcggacgatg
     1681 cgttcggaga tttcttcgag ttgcagcttc gtcttcgcga cgaccgcggc tggagcccgc
     1741 gacaggaagc tctcatcggc gaggcgcgcg cggatcttct ccgcttcggc ctccagcttc
     1801 tcgacgtccc tgcgcatctt ctccgcgtct tcagaggaga tcttctgctt cggaagatcc
     1861 acgacgatcc cagcgtccgc gatgacgtcg cgatgcgctc ctggccaatt cacactcgaa
     1921 gaaatctcca cggcgctcag gcgcgcgaga tggctgagga gcgggacgcg acgctgcatt
     1981 tcctctgaaa ggggtcctgg cgggagctcg attccgagcc gcagcgcttc ggtctgcggg
     2041 aggccgcgtt cggccctgag atttcggact ctcgtcacga tcgcccggag gagttcgacg
     2101 acctccaccg ccgcgtcgtc tttccactcg ttccggggct ccgggaactt cgccatcgtg
     2161 agctgcagtc cgtcgccgtt cagcgcctcg cggatctcgc acgacacgaa cggcatgaag
     2221 gggtgcagga gcgcgagcga gtcgaggagg acgcgcttca agacgccgcg cgtcttcgcc
     2281 ttctcggcct cgggcacgtc cgccccgcgc agcaccggct tgatcatctc gacgtagccg
     2341 tcgcagaact cggaccagaa gaactggtag agcgcctggg ccgcctcgtc gaaacggtac
     2401 gcctcgaggg acgcgttcac gcggcccgcg gcgtccgaaa ggcgcgcgag gatccagcgg
     2461 tccaccgtgg agagcgcccg gaagtcgagc gcctgggggt ccgcacactc gccttccgcg
     2521 atcaggggca gcgcgaagcg cgtcgcgttc cagaccttcg tcgcgaaggc acggtagccc
     2581 gcgacgcggg tcttgccgaa cgggaggtcc cggcccgtgc ccgagaggac ggcgagcgtg
     2641 aagcggacgg cgtccgcgcc gaactcgtcg caca
//
LOCUS       DAOMFY010000396         2665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_17423_length_2665_cov_6.196169, whole genome shotgun sequence.
ACCESSION   DAOMFY010000396 DAOMFY010000000
VERSION     DAOMFY010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17423_length_2665_cov_6.196169"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="PLB01_19135"
     CDS             complement(<1..1722)
                     /locus_tag="PLB01_19135"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HQQ79468.1"
                     /translation="MSAPDAAPAVRFDRRDRLFVLACLAVIVLGAAVGRAGFTRAFPE
                     ASIDFKVTREEAVRRGADALKARGFSVDGMRALATFDVDDEAKVFLERTLGLRKASPL
                     FATDVPVWRWAVRWVTPLEKLEYRSAVAPDGRVLSVRRLLPEKSAAPDPGDEAARALA
                     EEALRKDRGLEPGSLRFIRAEVEKRPARTDRVFEWESNTIRFGDAAMRYLVEVQGDRV
                     GRTSLHLEVPEKWRRDYQTLRSKNLAAGSVATFGLILTFGAVLVVFLQRVRRKDVKWR
                     LALTFAAVGAVLQLLASLNELPIRLYDYDTAEAWSGFLAMALLSDVAGAIALGAALFL
                     LVAAGEPLFREAYPGQPALGRILSRTAFRSKRFFRGLLLGYAMTAFFFAYQVVFYIVA
                     ERLGAWAPADVPYSSLLGTSFPWLGVLVMGFMPATTEEFSSRMFSIPFVRQFAPGWVA
                     VALPAFVWGFAHSTYPNQPFYIRGLEVGFAGVLIGLVFLREGMLPLLVWHFTVDAVYT
                     ALLLVRSTNPYFVVSGTLAALVLLLPMAVALILYFRSGGFAPDDGLSNAAVGSAPAPP
                     PAVEHAPA"
     gene            complement(1719..>2665)
                     /locus_tag="PLB01_19140"
     CDS             complement(1719..>2665)
                     /locus_tag="PLB01_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79469.1"
                     /translation="AALLGVPGAEDDGAESALDRLLDFARRAASSDNVLLVHAVAPDP
                     QHAASQLLRKAARSADLSCATEDVRRERLHALGLERAIDRKVLVEAEVFELLTSHGRL
                     MARPFLSDLDRLIDSAEGPRVTAEHAARQVADESREYGSDFVDAVAARDMLEALRLLG
                     KLLSGATFAAFRPFGGKEEAPQAAKKGPRGEAAFFPLLGLLAGEVRRMLALRAALEER
                     GASMRRADYRSFADRILPALKTPKPGLPPLPLEGHPFVLHKAYLASLNWTADELADAL
                     VGIEAIDRGVKTGAGSGSELLEAWLLSRARPAAQRAAG"
BASE COUNT          429 a          923 c         1019 g          294 t
ORIGIN      
        1 ggccggcgcg tgctcgacgg ccggcggcgg cgcaggcgcc gagccgaccg cggcgttcga
       61 gagaccgtcg tccggcgcga accctccgct gcggaaatac aggatcagcg cgacggccat
      121 cgggagcagc aggacgagcg ccgcgagggt gccggagacc acgaagtagg ggttcgtcga
      181 gcgcacgagg agcagcgccg tgtagacggc gtccaccgtg aagtgccaga cgaggagcgg
      241 gagcatgccc tccctgagga agacgagccc gatcaggact cccgcgaacc ccacctcgag
      301 cccgcggatg tagaagggct ggttcgggta cgtcgagtgc gcgaagcccc agacgaacgc
      361 gggcagggcg accgcgaccc agccgggcgc gaactgccgc acgaacggga tcgagaacat
      421 ccgcgacgag aactcctcgg tcgtcgcggg catgaagccc atgacgagga cgccgagcca
      481 cgggaacgac gtcccgagga ggctcgagta gggaacgtcc gccggcgccc aggcgccgag
      541 ccgctccgcg acgatgtaga agacgacctg gtacgcgaag aagaacgccg tcatcgcgta
      601 cccgaggagg aggccgcgga agaacctctt cgaacggaag gccgtccggg acaggatcct
      661 cccgagcgcg ggctggccgg gatacgcctc gcggaacagc ggctcgcccg ccgcgaccaa
      721 gagaaagagc gcggccccga gcgcgatcgc gcccgcgacg tcggagagga gcgccatcgc
      781 gaggaagccg gaccaggcct ccgccgtgtc gtagtcgtag aggcggatag ggagctcgtt
      841 gagcgacgcg aggagctgga gcacggcgcc gaccgccgcg aacgtcagcg cgagcctcca
      901 cttcacgtcc ttgcgccgga cgcgctggag gaagacgacg agaaccgcgc cgaacgtgag
      961 gatgaggccg aacgtcgcga cgctgcccgc cgcgaggttc ttcgaacgga gcgtctggta
     1021 gtcgcgccgc cacttctcgg ggacctcgag gtgcagcgac gtccgcccga cgcggtcgcc
     1081 ctgcacctcg acgaggtagc gcatcgcggc atcgccgaaa cggatcgtgt tcgactccca
     1141 ctcgaagacg cggtccgtcc gcgcgggccg cttctcgacc tcggcccgga tgaaccgcag
     1201 cgagccgggc tcgaggccgc ggtccttccg gagcgcctct tcggcgagag cgcgcgcggc
     1261 ctcgtcgccg ggatcgggcg cggcggactt ctcggggagg agccggcgga cggagaggac
     1321 gcgcccgtcg ggcgcgacgg cggagcggta ctccagcttc tcgagcggcg tcacccagcg
     1381 gaccgcccag cgccacacgg ggacgtccgt cgcgaagagc gggctcgcct tccggaggcc
     1441 gagcgtcctc tcgagaaaca ccttcgcctc gtcgtcgacg tcgaacgtcg cgagggcccg
     1501 catgccgtcg acggagaaac cgcgcgcctt cagcgcgtcc gcgccgcgcc tcacggcctc
     1561 ttcccgcgtg accttgaaat cgatggaggc ctcggggaag gcgcgcgtga accccgcgcg
     1621 cccgacggcc gcgccgagga cgatcaccgc gaggcatgcg agcacgaaga ggcggtcgcg
     1681 ccggtcgaag cggacggcgg gggcggcgtc cggcgcgctc atccggcggc tcgctgggcg
     1741 gcggggcgcg cgcgtgaaag gagccacgcc tcgaggagct cgctcccgga gcccgcgccg
     1801 gtcttgacgc cgcggtcgat cgcctcgatt ccgacgagcg cgtcggcgag ctcgtccgcg
     1861 gtccagttca gcgaggcgag gtaggccttg tggaggacga acgggtggcc ctcgagcggc
     1921 agcggaggga ggccgggctt cggcgtcttc agcgcgggca ggatgcggtc cgcgaagctg
     1981 cggtagtccg cgcggcgcat cgacgcgccc cgctcctcga gagcggccct gagcgcgagc
     2041 atccgccgca cctctcccgc gaggaggccg aggaggggga agaacgccgc ctcgccgcgc
     2101 ggacccttct tcgcggcctg gggcgcttcc tccttgccgc cgaacggccg gaaggccgcg
     2161 aacgtcgcgc ccgagaggag cttgccgagg aggcgcaggg cctcgagcat gtcgcgggcc
     2221 gcgacggcat ccacgaagtc cgagccgtac tcgcggctct cgtccgcgac ctgacgcgcg
     2281 gcgtgctcgg ccgtgacgcg cgggccctcg gccgagtcga tgaggcggtc gaggtccgac
     2341 aggaagggcc gcgccatgag gcgcccgtgg ctcgtcagga gctcgaacac ctccgcctcg
     2401 acgaggacct tgcggtcgat cgcacgctcg aggccgagtg cgtggagacg ctcgcggcgg
     2461 acgtcctcgg tcgcgcacga gaggtccgcg gagcgggcgg ccttgcgcag cagctgcgag
     2521 gcggcgtgct gcgggtcggg cgccacggcg tggacgagga ggacgttgtc cgacgaggcc
     2581 gcgcgacgcg cgaagtccag gagccggtcg agcgccgact cggcgccgtc gtcctcggcg
     2641 cccgggacgc cgagaagcgc cgcga
//
LOCUS       DAOMFY010000397         2653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_17541_length_2653_cov_6.995381, whole genome shotgun sequence.
ACCESSION   DAOMFY010000397 DAOMFY010000000
VERSION     DAOMFY010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17541_length_2653_cov_6.995381"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLB01_19145"
     CDS             complement(<1..750)
                     /locus_tag="PLB01_19145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HQQ79470.1"
                     /translation="MTDHTHTPDLRDRLSGQQGRAFWRSLEEYAGTPEFAEMLGREFP
                     QHASEWDEGVSRRRFLELSAASLSLAGLSACVKQPREGLVPYVKQPEEVVPGRPLFFA
                     TSATLGGYATGLLVESHTGRPTKVEGNPDHPASLGATDACAQALVLSLYDPDRSQTIT
                     RLGQIRTWANFTDELAARVKIHAALQGDGFRILTQTVTSPTLASQIRAVLAAMPKARW
                     HQWEPAGRDAARAGARLAFGQAVETRHDFGKA"
     gene            complement(747..1403)
                     /locus_tag="PLB01_19150"
     CDS             complement(747..1403)
                     /locus_tag="PLB01_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQQ79471.1"
                     /translation="MSQIFNRSTNAIARLSIWGAVFVLAGVAWAASVLNRSAYNTNQG
                     VTIKQPIPFGHEHHVAGLGIDCRYCHSSVEKSASAGIPPTATCMNCHKLVWNDSPMLA
                     PVRESFRTGQPLNWARVYDLPDFVYFDHSIHVAKGIGCVSCHGRVDKMRLTYQYPSLQ
                     MEWCLECHRNPEKNLRPKDRIFDLAWKADDQAALGAALKKEYGIRSAYALTNCSTCHR
                     "
     gene            1512..>2653
                     /locus_tag="PLB01_19155"
     CDS             1512..>2653
                     /locus_tag="PLB01_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79472.1"
                     /translation="MPSSPTRARLAAPLAVFALVFALRGAALAALLATPLASWHLWTE
                     TDEHAYVEWSARLAAGNWADVPAYRAYFNWQREYGPPEAWERWYQKNAYYAGPLYPYG
                     LAVLRKVFGSPFLPARLLQLLLACLAAAAVAAAVQRLGEDFLRRGRGEGGRPVLWSSI
                     GAGVLYGSYGPLVFHDFFLYRDGPVAHLATLLVVLPMIFGGGAAFGLGLLGGAAVLMK
                     QTVLPLALFSLYSLSKKSESPGGAQRRVLVLGAMGLAIPLGVLAVRNVSAGVPPLTFD
                     TRQAIGIVWGNGFGADATTNPPPRMKEILEEAAGSTSKTARLVLAGYGEQPLELPKLL
                     LKKLATFFNRFEVPDNANFYFFRDRLRILEMLPVFPCLLGIGGVGV"
BASE COUNT          359 a          871 c          959 g          464 t
ORIGIN      
        1 ggccttgccg aagtcgtggc gcgtttcgac ggcctggccg aaggcgaggc gggcgcccgc
       61 gcgggctgcg tcgcgccccg ccggctccca ctggtgccag cgggccttcg gcatcgccgc
      121 gaggaccgcg cggatctggg acgcgagcgt cggggacgtg acggtctgcg tcaggatgcg
      181 gaagccgtcg ccctgaaggg ccgcgtgtat cttcacgcgc gccgcgagct cgtccgtgaa
      241 gttcgcccac gtgcggatct ggccgaggcg cgtgatcgtc tgggagcggt ccgggtcgta
      301 gagggagagg acgagcgcct gggcgcacgc gtccgtcgcg ccgaggctcg ccgggtggtc
      361 cggattgccc tcgaccttcg tcgggcggcc cgtgtggctc tcgacgagga ggcccgtcgc
      421 gtagccgccg agcgtcgcgg aggtcgcgaa gaagagcggg cggccgggga cgacctcctc
      481 gggctgcttc acgtacggga cgaggccctc gcggggctgc ttgacgcagg ccgagaggcc
      541 ggcgagggag agcgaggcgg cggagagctc gaggaagcgg cggcggctca cgccctcgtc
      601 ccactcggac gcgtgctgcg ggaactcgcg gccgagcatc tcggcgaact cgggcgtccc
      661 ggcgtactcc tcgaggcttc gccagaaggc gcggccctgc tgcccggaga ggcgatcccg
      721 gagatcgggg gtgtgcgtgt ggtcggtcat cggtggcacg tgctgcagtt cgtgagcgcg
      781 taggcggagc ggatgccgta ctccttcttg agggcggccc cgagggcggc ctggtcgtcg
      841 gccttccatg cgaggtcgaa gatcctgtct ttcggccgga ggttcttttc cgggttgcgg
      901 tggcactcga ggcaccactc catctggagc gacgggtact ggtacgtgag gcgcatcttg
      961 tccacccggc catggcagga gacgcagccg atgcccttcg ccacgtggat cgagtggtcg
     1021 aagtagacga agtcggggag gtcgtagacg cgcgcccagt tcagcggctg tccggtccgg
     1081 aagctctcgc gcaccggcgc gagcatcggg ctgtcgttcc acacgagctt gtggcagttc
     1141 atgcacgtcg cggtcggcgg gatgccggcc gaggcgctct tctcgacgga cgaatggcag
     1201 tagcggcagt cgattccgag gccggccacg tggtgctcgt gcccgaaggg aatcggctgc
     1261 ttgatcgtca cgccctggtt cgtgttgtag gcggaacggt tcaggacgga cgccgcccac
     1321 gcgacgccgg cgaggacgaa caccgctccc cagatggaca ggcgggcgat ggcattcgtg
     1381 gaccgattga agatctgcga catgactctg tttcttggcc gggctcgccg gccgggaagg
     1441 ccgggaattt accagatcgc gtctgagagt gagttgccgt accccatcga cgcgcgcgcc
     1501 tagaatcaat cgtgccgtcg tctccgacac gcgcgcgtct ggcggctccg ctcgccgtgt
     1561 ttgcgctcgt cttcgcgctg cgcggggccg cgctggccgc gctcctcgcg acgcccctcg
     1621 cctcctggca tctctggacg gagacggacg agcacgccta cgtcgagtgg tcggcccgtc
     1681 tcgcggccgg caactgggcc gatgtccccg cctaccgcgc gtacttcaac tggcagcggg
     1741 agtacgggcc gccggaggcg tgggagcgct ggtaccagaa gaacgcctac tacgcggggc
     1801 cgctgtaccc ctacggcctc gcggtcctcc ggaaggtctt cgggtcgccg ttcctgccgg
     1861 cgcggctgct gcagctcctg ctcgcgtgcc ttgccgcggc ggcggtcgcc gccgcggtac
     1921 aaaggctcgg agaagatttt cttagaaggg gaagagggga aggcggccgc ccggttttgt
     1981 ggtcgtcgat cggtgcgggt gtgctctacg gaagctacgg cccgctcgtc ttccacgact
     2041 tctttctcta ccgcgacggc cccgtcgcgc atctggcgac gctcctcgtc gtgctgccga
     2101 tgatcttcgg cggaggcgcc gccttcggcc tcgggctcct gggcggcgcc gccgtcctca
     2161 tgaaacagac ggtccttcct ctggccctct tctctctgta ttcgctttca aagaaatccg
     2221 aatccccggg cggcgcgcag cgccgcgttc tcgtcctcgg cgcaatgggc ctcgcgatcc
     2281 cactcggcgt tctcgccgtg cggaacgttt ccgccggcgt gccgccgctg accttcgaca
     2341 cgcggcaggc gatcgggatc gtgtggggca acgggttcgg cgcggacgcg acgacgaacc
     2401 cgccgccgcg catgaaggag atcctggaag aggccgccgg ctcgacgtcg aagaccgcgc
     2461 ggctcgtcct cgcgggctac ggggagcagc cgctcgaatt gccgaagctg ctcctgaaga
     2521 agctcgccac gttcttcaac cggttcgagg tccccgacaa cgcgaacttc tacttcttca
     2581 gggatcgatt gaggattctc gaaatgctcc ctgtctttcc atgcctcctc ggcatcgggg
     2641 gagtcggggt ttt
//
LOCUS       DAOMFY010000398         2633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_17771_length_2633_cov_6.876649, whole genome shotgun sequence.
ACCESSION   DAOMFY010000398 DAOMFY010000000
VERSION     DAOMFY010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17771_length_2633_cov_6.876649"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..386
                     /locus_tag="PLB01_19160"
     CDS             129..386
                     /locus_tag="PLB01_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQQ79473.1"
                     /translation="MAVDKELLDILACPACDARPAVTLCTLRGDVAKAVVEKYRESLK
                     DEEPVVTQGLSCAACGRVYPIVSDIPVMLVEEALPADAREA"
     gene            451..1605
                     /locus_tag="PLB01_19165"
     CDS             451..1605
                     /locus_tag="PLB01_19165"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQQ79474.1"
                     /translation="MFFLLGIGCVPGIGIANVCLALCLASAVAWRWQARRMGEDFLGP
                     FRRTTPVHAPIAAFVLLSVVSCFFSTLPSKSLLQIKGFGTFLLVVFAAALLDDEADVK
                     LAVDVWRTTALYLVLRGFAEWISGSPSLDFRIAGGLSVYMTYAGLLLVFVPLLGARGV
                     SGGSSRARWADVAVALAGAVAMALTLTRGAYLGLAVGVVAVLLAARPRLALLAPIAIA
                     LFFVLMPLPVRDRLASATNPRDVTMNDRVAMWRAGRAMISDHPLTGVGPGRVKPLYPS
                     YRVPGFVNPIPGHLHNNLVMIAAETGIPSLLAYLAFVGAFFLGALRILRRSPRGDPAR
                     GVTLGAIGAMAALFAAGMFEYNFGDVEVLMATLVVATLPFAAARAGERAD"
     gene            1615..>2633
                     /gene="xseA"
                     /locus_tag="PLB01_19170"
     CDS             1615..>2633
                     /gene="xseA"
                     /locus_tag="PLB01_19170"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQQ79475.1"
                     /translation="MSSRFPGRAYPLRGAAPRRVAETQLSLGDAGPDATAMTVSQLVE
                     RMNGILKGGIPDVWVKGEIADIKRPGSGHLYFSLKDERALMPCVMWRSTAVRLPFEAE
                     EGLLVLARGYPDVYAPYGKLSLVVSELQPSGVGALQLAFEQLKARLAAEGLFDAAKKR
                     PLPLLPRRIGVVTSRHGAALRDILKVLDARFPNAHVTLYPASVQGAAAPGEIVRAVRA
                     FSRVAGSADVVIVARGGGSKEDLAAFNDESVVRAVASSNVPVICAVGHETDVSLAELA
                     ADVRAATPSQAAEIVVGPLAQFEERLARAKRDLKISLKVRMDRAARDLARLRSAPALA
                     GFPAAV"
BASE COUNT          309 a          961 c          917 g          446 t
ORIGIN      
        1 cgtggcgcgc ttttgcggcc gccggcgtcg cggccggggc cgccgcgtcg ttcgccggga
       61 ccgcggcgct cgccgccgtg tggttcctcg cgtcgcggcg ggccgcggaa ccctgctagg
      121 atcacctcat ggccgtggac aaggaactgc tcgacatcct cgcgtgtccg gcctgcgacg
      181 cgcggcccgc cgtgaccctg tgcacgctcc gcggcgacgt cgcgaaggcg gtcgtcgaga
      241 agtaccgcga gtccctgaag gacgaggaac cggtcgtgac gcaggggctg tcctgcgccg
      301 cctgcgggcg cgtctacccg atcgtttcgg acatcccggt gatgctcgtc gaggaggcgc
      361 ttccggcgga cgcccgggag gcctgaccct cccctgaggc tctaccccga accgatcgcg
      421 cgccggagcg gattcgaacg cgcgacgttc gtgttcttcc tgctcggcat cggatgcgtg
      481 ccgggcatcg gcatcgcgaa cgtctgtctc gcgctctgcc tcgcatcggc cgtcgcgtgg
      541 cgctggcagg cgcggaggat gggcgaggac ttcctcgggc cgttccgccg gacgacgccg
      601 gtccacgcgc ccatcgccgc gttcgtcctc ctctccgtcg tctcgtgctt cttctcgacg
      661 ctcccgtcga agtcgctcct ccagatcaag ggcttcggaa cgtttctcct cgtcgtcttc
      721 gcggcggcgc tcctcgacga cgaggcggac gtcaagctcg ccgtggacgt ctggcgcacg
      781 accgcgctct acctcgtcct gcgcggcttc gcggagtgga tctccggctc gccgagcctc
      841 gacttccgga tcgcgggcgg gctctccgtc tacatgacgt acgcgggcct cctcctcgtc
      901 ttcgtgccgc ttctcggagc gcgcggagtc tccggagggt cgtcgcgggc ccggtgggcc
      961 gacgtcgccg tcgccctcgc cggcgccgtg gcgatggctc tcacgctgac gcgtggcgcc
     1021 tacctgggcc tcgcggtcgg cgtcgtcgcg gtgctcctcg cggcgcggcc gcgcctcgct
     1081 cttctcgcgc ccattgcgat cgcgctcttc ttcgtcctga tgccgctgcc cgtgcgggac
     1141 cggctggcgt ccgcgacgaa cccccgcgac gtgacgatga acgaccgcgt cgcgatgtgg
     1201 agggcgggcc gcgccatgat ctcggatcac ccgctcacgg gcgtcgggcc ggggcgcgtc
     1261 aagccgctct atccctcgta ccgcgtgccg ggattcgtca acccgatccc ggggcacctc
     1321 cacaacaacc tcgtcatgat cgcggccgag acggggatcc cctccctgct cgcgtacctc
     1381 gcgttcgtcg gggcgttctt cctcggggcg cttcggatcc tccgccgctc gccgcgcggc
     1441 gaccccgcgc ggggcgtcac gctcggggcg atcggcgcga tggccgctct cttcgccgcc
     1501 gggatgttcg agtacaactt cggcgacgtc gaggtcctga tggcgacgct cgtcgtcgcg
     1561 acgctgccct tcgcggcggc ccgcgccggc gagcgggccg actagaatcg gccgatgtcg
     1621 agccgctttc ccgggcgcgc ctatcccctc aggggcgccg cgccgcgccg cgtcgccgag
     1681 acgcagctct cgctcgggga cgccgggccg gacgcgacgg cgatgacggt gtcgcaactg
     1741 gtcgagcgga tgaacggaat cctgaagggc ggaattccgg acgtctgggt gaagggcgag
     1801 attgcggaca tcaagcgccc cggctcgggc catctttatt tttctttgaa ggatgagcgg
     1861 gctttgatgc cttgcgtgat gtggcggagc acggccgtcc gtctaccatt cgaagcagaa
     1921 gaaggacttc tcgtcctggc gcgcggatac ccggacgtgt acgcacccta cggcaagctg
     1981 tctctcgtcg tctccgagct ccagccttcg ggcgtcggcg cgctgcagct cgcattcgag
     2041 cagctgaagg cgcgactcgc cgccgaaggg ctcttcgacg ccgccaagaa acgtccgctg
     2101 ccgctcctcc cgcggcgcat cggcgtcgtg acgtcgcgcc acggggccgc gctgcgcgac
     2161 atcctgaagg tcctcgacgc gcgcttcccg aacgcgcacg tgaccctcta cccggcctcc
     2221 gtccagggcg cggcggcgcc gggggagatc gtccgcgccg tgagggcgtt ctcgcgggtg
     2281 gcgggatcgg ccgacgtcgt catcgtcgcg cgagggggcg gctcgaagga agacctcgcc
     2341 gcgttcaacg acgagtcggt cgtccgggcc gtggcctcct cgaacgtccc ggtgatctgc
     2401 gccgtgggcc acgagaccga cgtctcgctc gcagagctcg cggccgacgt ccgggcggcc
     2461 acgccctcgc aggccgccga gatcgtcgtc ggcccgctcg cgcaattcga ggaacgcctc
     2521 gcccgcgcga agagagactt gaagatttct ctgaaggtga ggatggacag ggccgcgcgc
     2581 gacctcgcgc ggctcaggtc ggcacccgcg cttgcggggt tccccgcggc cgt
//
LOCUS       DAOMFY010000399         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_17783_length_2632_cov_7.133489, whole genome shotgun sequence.
ACCESSION   DAOMFY010000399 DAOMFY010000000
VERSION     DAOMFY010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17783_length_2632_cov_7.133489"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLB01_19175"
     CDS             complement(<1..1283)
                     /locus_tag="PLB01_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79476.1"
                     /translation="MSASASSPRPFGPYVLTRAIGSDPFGEVWRGGTAGAPRLHPFYL
                     FRTFTSAAVDRAALLAAMETAVHLVDEVRGPAVAKGTVMGVVDDTPFLAIEYVEGRTL
                     DALFGARVLGAVMPAEHGLLIAERLLTALEAGAPVERFTGAPHGFLVPSFVSVSNEGE
                     ARVFGYGLGPGLLPSLGVARARQAFAPYIAPEVLASGKPGVPGDLYSVGAILFEALTG
                     KPPLPGVALEAVDTSTLAVDGSPVPEDIRRLLRRVLHPDPATRDRSIAGFRRDLSALL
                     YGGPYAPSSFNLAFFLQKNFERAIQREQAEIGAEEALDPAAIAASRSTSETSGRRRVE
                     SSGSGPKADPPAPARKVDSTTGSRRAPVVPPQPVRPAPAKGDAPLRTMTPPPKKPLGG
                     VPVWAVALSAVVVLAAVGRGEIPALPDRDDLPEPDP"
     gene            complement(1327..2397)
                     /gene="mltG"
                     /locus_tag="PLB01_19180"
     CDS             complement(1327..2397)
                     /gene="mltG"
                     /locus_tag="PLB01_19180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQQ79477.1"
                     /translation="MKRTAAIHKRHLIPLLVWVALFALLAGAVGFGLLLYGVRAPYKG
                     YTGDEVIVSIAPRTPSATVFAHLESVGVLRDWRLGLVALKVLHRGKTLKAGEYRFAGP
                     RAPDQVVLALVAGDVVTYRITVPEGFAAEEIFALFYSQGFAAQRDYEFLFTRPAEFEG
                     VPAGAPTLEGFLFPDTYTVTRSMTAREILSAMTRQFARRLPADFAEKAKSEKLGILEA
                     TTLASLVEKETALPAERPIVAGVYRNRLKAGMLLQCDPTTIYALKRLGRWRGALARSE
                     LTVDEPYNTYVRAGLPPGPICSPGLASLRSAVAPADVDYRFFVAAGDGSHLFSRDYED
                     QQRNVTRYHQAKRAAREERATP"
     gene            complement(2408..>2632)
                     /gene="ruvX"
                     /locus_tag="PLB01_19185"
     CDS             complement(2408..>2632)
                     /gene="ruvX"
                     /locus_tag="PLB01_19185"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQQ79478.1"
                     /translation="ARDDQRLDQHFARRLGEASGLPVDLHGEALTSVAADAALDDAGV
                     PARGRAGLRDAEAAAVLLRDWFAAHPGGSA"
BASE COUNT          379 a          887 c         1027 g          339 t
ORIGIN      
        1 ggatccggct ctggcaggtc gtcccggtcc ggcagcgcgg gaatttcgcc gcgcccgacc
       61 gcagccagga cgacgacggc gctgagtgcc accgcccaca cggggacgcc gcccagcggc
      121 ttcttcgggg gaggcgtcat cgtccggagt ggcgcgtcgc ctttcgcggg cgcgggccgc
      181 acgggctgcg gaggcacgac gggcgcgcgg cgcgagcccg tcgtcgagtc cacctttcgt
      241 gccggtgcgg gagggtccgc tttcgggccg ctcccgctcg actcgacacg gcggcggccg
      301 ctcgtctcgg aggtggaccg ggacgccgcg atcgcagcag ggtcgagcgc ctcttcggcg
      361 ccgatctccg cctgctcgcg ctggatcgcg cgctcgaagt tcttctggag gaagaacgcg
      421 aggttgaagg acgacggcgc gtagggaccg ccgtaaagaa gcgccgagag atcgcgccgg
      481 aagcccgcga tgctgcggtc gcgcgtcgcg gggtccgggt ggagcacacg gcgcaggagg
      541 cgccggatgt cctcgggaac cggcgagccg tccacggcga gcgtggacgt gtcgaccgct
      601 tcgagcgcga cgccgggaag agggggcttg cccgtgagcg cctcgaagag gatggcgccg
      661 acgctgtaga ggtcgcccgg caccccgggc tttccggacg cgaggacctc gggcgcgatg
      721 tagggcgcga acgcctggcg ggcgcgcgcg acgccgagcg agggaaggag acccggcccg
      781 aggccgtacc cgaagacgcg cgcctcgccc tcgttcgaca cggagacgaa cgacggcacg
      841 aggaagccgt gcggggcgcc cgtgaaccgt tcgacggggg cgccggcctc gagcgcggtc
      901 aggaggcgtt cggcgatgag gagcccgtgt tcggcgggca tcacggcccc gaggacgcgc
      961 gcaccgaaga gcgcgtcgag cgtgcggccc tcgacgtact cgatcgcgag gaacggtgtg
     1021 tcgtcgacga cgcccatgac cgtgcccttc gcgacggccg gcccccggac ctcgtcgaca
     1081 aggtgcacgg ccgtctccat cgcggcgagg agggccgccc ggtccacggc cgcggacgtg
     1141 aaggtccgga agaggtagaa ggggtgcagc cggggcgctc ccgccgtccc gccgcgccac
     1201 acctctccga acggatcgct tccgatcgcg cgcgtcagga cgtacggccc gaaaggccgc
     1261 ggactggatg cggaagcgct catcagactc cggtcgggtg cgtctcagtc taccagtccg
     1321 atccggtcag ggagtcgcgc gctcctcgcg ggccgcccgc ttggcctggt ggtatcgggt
     1381 cacgttgcgc tgctggtctt cgtagtcgcg cgagaagagg tgcgagccgt ctcccgcggc
     1441 cacgaagaag cgatagtcga cgtccgcggg cgcgaccgcg gacctcagcg atgcgagccc
     1501 ggggctgcag atcggaccgg gcggaaggcc ggcgcgcacg taggtgttgt agggctcgtc
     1561 caccgtgagc tccgagcgcg cgagcgcgcc gcgccaccgc ccgagccgct tgagggcgta
     1621 gatcgtggtc gggtcgcact ggaggagcat tccggccttc agccggttgc gatagacgcc
     1681 cgcgacgatc gggcgttcgg cggggagggc ggtctccttc tccacgagcg acgcgagcgt
     1741 cgtcgcctcg aggatgccga gcttttccga cttggccttc tcggcgaagt cggccgggag
     1801 gcggcgcgcg aactgtcgcg tcatggcgga caggatctcc cgggcggtca tcgagcgcgt
     1861 gaccgtgtac gtgtcgggga acaggaagcc ttcgagcgtc ggggcgccgg cggggacgcc
     1921 ctcgaactcg gccgggcgcg tgaagaggaa ttcgtagtcc ctctgggctg cgaagccttg
     1981 cgagtagaag agcgcgaaga tctcctcggc ggcgaagcct tcgggcaccg tgatccggta
     2041 cgtcacgacg tcgcccgcga cgagcgcgag gaccacctgg tcgggggcgc gcggccccgc
     2101 gaagcgatat tcgccggcct tgagcgtctt gccgcggtgg agaaccttca gggcgacgag
     2161 gccgagccgc cagtcgcgca ggacgccgac gctctcgagg tgcgcgaaca cggtggccga
     2221 cggcgtccgc ggcgcgatcg atacgatgac ctcgtcgccc gtgtagccct tgtacggggc
     2281 gcggacgccg tagagcagga gcccgaagcc gaccgcgccc gcgaggagcg cgaagagggc
     2341 gacccacacg aggagcggga tgaggtggcg cttgtggatc gcggccgtgc gcttcaccgt
     2401 gggcctctca cgcggatcct cccgggtgcg cggcgaacca gtcccggagg aggaccgcgg
     2461 cggcctcggc gtccctcagc ccggcccggc cgcgggcggg aacgcccgcg tcgtcgagcg
     2521 ccgcgtcggc ggcgacggac gtgagcgcct cgccgtgcag atcgacgggg agcccggagg
     2581 cctcgccgag ccggcgcgcg aaatgctgat cgaggcgctg atcgtcgcgc gc
//
LOCUS       DAOMFY010000400         2588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_18338_length_2588_cov_6.232531, whole genome shotgun sequence.
ACCESSION   DAOMFY010000400 DAOMFY010000000
VERSION     DAOMFY010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18338_length_2588_cov_6.232531"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLB01_19190"
     CDS             <1..1262
                     /locus_tag="PLB01_19190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQQ79479.1"
                     /translation="IKGDVEGMIVTFNRTLKVRRKFTNDGRDLSDILKQIEEESALGT
                     QIDSERRDVIKAIDDSTSADQAVGRVRGYVQSLENDLKFTFDAMKTTLNQLSGVEGRK
                     IMVHVSGGLPQSPGLELWSYIQARYPNSTSYSMGQFEFDKTTSYLGIIQAANAAGVSM
                     YAIDAGGLTVDSNVTAEQRTTSQRIDTFLEKNNLQNMLILMAEETGGRAIINRNDVGI
                     ALKDMEKDYTSYYSLGYRSVRSGLDRPHKVDVKLKAKKGLTVLARRSYVEKGFDTKVR
                     EGVTSALFFSRDENPLGAALAVGDPVPDRGNFLVPIQIRVPFSRIALLPEGTKVRGRL
                     VFYFVVVDSDGKQSELTSQPVLVDLDAKTLDAQSKRDFLYDVKLVMIPGGQKLSLAIR
                     DDVTNTTSYLQKSIFVSAFAGERPAKR"
     gene            1267..1953
                     /locus_tag="PLB01_19195"
     CDS             1267..1953
                     /locus_tag="PLB01_19195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HQQ79480.1"
                     /translation="MIGPVKRVILFAREPVAGQVKTRLAREIGGAPAAALYGAFLQDL
                     AAALPDPRWDAVLAHAEFEPGPYLVGTFRAPWDLVPQGDGSLGERLARAVVRARMEGK
                     RDVVVAGSDAPTLGREDLESAFAALEHEADVVFAPAPDGGFSLVGLAGNWDFRDFFEG
                     VRWSTANALEDARRAAMARGARVTLIATLPDVDEVKDLVALSPYFSADPTLAPATRRA
                     LGVLLGPDRP"
     gene            1950..>2588
                     /locus_tag="PLB01_19200"
     CDS             1950..>2588
                     /locus_tag="PLB01_19200"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HQQ79481.1"
                     /translation="MRVVSADESRALDVRTIAAGTPGIVLMKRAASAAVREIAGVFAR
                     RPSLAARVVVLAGPGNNGGDGFEVARLLMAGRIASNVETLLLGNPLHLTPDARKTYHR
                     LEQVGGAIREVNDARELEPLAVATLVVDALFGTGLKRRVAPDGLPARAIALASSARAF
                     VVSVDLPSGLSADEAVPWEPSVRADLTVTFGWPKPCHVERAPGARCGRVAVAD"
BASE COUNT          389 a          916 c          891 g          392 t
ORIGIN      
        1 ccatcaaggg cgacgtcgaa ggaatgatcg tgaccttcaa ccggacgctc aaggtccgga
       61 ggaagttcac gaacgacgga cgcgacctct cggacatcct caagcagatc gaggaggagt
      121 cggccctcgg gacgcagatc gactccgagc gccgcgacgt catcaaggcg atcgacgact
      181 cgacctcggc cgaccaggcc gtcggccgcg tgcggggcta cgtgcagtcc ctcgagaacg
      241 acctcaagtt tacgttcgac gcaatgaaga ccaccctcaa ccagctctcg ggcgtcgagg
      301 gccggaagat catggtccac gtgtcgggcg ggctcccgca gtcgccgggt ctcgagctgt
      361 ggtcctacat ccaggcccgg tatccgaact cgacgtccta ctcgatgggg cagttcgagt
      421 tcgacaagac cacgagctac ctcgggatca tccaggctgc gaacgccgcg ggcgtctcga
      481 tgtatgcgat cgacgccggg gggctcacgg tcgactcgaa cgtgacggcc gagcagcgca
      541 cgacgtccca gcgaatcgac acgttcctcg agaagaacaa cctccagaac atgctcatct
      601 tgatggccga ggagacgggc gggcgcgcga tcatcaaccg caacgacgtc ggaatcgccc
      661 tgaaggacat ggagaaggac tacacgtcct actactcgct cgggtaccgc agcgtgcgct
      721 cgggcctgga ccggccgcac aaggtggacg tgaagctgaa ggcgaagaag ggcctgacgg
      781 tcctcgcccg gcggagctac gtcgagaagg gcttcgacac gaaggtccgc gagggtgtca
      841 cgtcggccct cttcttctcc cgcgacgaga acccgctcgg cgcggccctc gcggtgggcg
      901 accccgtccc cgaccgcggc aatttcctcg tcccgatcca gatccgcgtg ccgttctcgc
      961 gcatcgcgct gctccccgaa gggacgaagg tccgcggccg gctcgtcttc tacttcgtcg
     1021 tggtcgattc cgatggcaag cagtccgagc tcacctccca gcccgtgctc gtggacctgg
     1081 acgcgaagac gctcgacgcc cagagcaagc gcgacttcct ctacgacgtg aagctcgtga
     1141 tgatcccggg cggccagaag ctctcgctcg cgattcgaga cgacgtcacg aacacgacga
     1201 gttacctgca gaagtcgatc ttcgtctcgg cgttcgcggg ggagcgcccg gccaagcggt
     1261 gaggccatga tcggacccgt caagcgcgtc atcctcttcg cccgcgagcc cgtggcgggg
     1321 caggtcaaga cgcgcctcgc gcgggagatc ggcggcgcgc ccgcggcggc cctctacggg
     1381 gcgttcctgc aggatctcgc cgcggcgctg ccggacccgc gctgggacgc ggtgctcgcg
     1441 cacgccgagt tcgagccggg cccgtacctc gtggggacgt ttcgcgcccc gtgggatctc
     1501 gttccgcagg gcgacggctc gctcggtgag cggctggccc gcgccgtcgt ccgcgcgcgc
     1561 atggagggca agcgcgacgt cgtcgtcgcg gggagcgatg cgccgacgct cggcagggag
     1621 gacctcgagt ccgccttcgc cgccctcgag cacgaggccg acgtcgtctt cgcgccggcg
     1681 cccgacggcg ggttctctct cgtggggctt gccggcaact gggatttccg ggatttcttc
     1741 gagggcgtcc gatggtccac cgcgaacgcg ctcgaagacg ctcgtcgtgc ggcgatggcg
     1801 agaggggcgc gcgtgaccct catcgcgacc cttccggacg tcgacgaagt gaaggatctc
     1861 gtggcccttt ccccctactt ttcggcggac ccgactcttg cgcccgcgac gaggagggcc
     1921 ctcggcgtcc tcctggggcc ggaccggccg tgagagtcgt ctcggccgac gagtcgcgcg
     1981 ccctcgacgt gcggacgatc gcggccggga cgcccgggat tgtcctcatg aagcgcgccg
     2041 cgagcgcggc cgtgcgggag atcgcgggcg tcttcgcccg ccggccgtcc ctcgccgcgc
     2101 gcgtcgtcgt gctcgccggc ccggggaaca acggcggcga cggcttcgag gtcgcgcgcc
     2161 tcctcatggc aggacggatc gcgtcgaacg tcgagaccct tctcctcggc aacccgctgc
     2221 acctcacgcc ggacgcgcgg aagacctacc accgcctcga gcaggtcggg ggcgcgatcc
     2281 gcgaggtgaa cgacgcgcgc gagctcgagc ccctggcggt cgccacgctc gtcgtggacg
     2341 cgctcttcgg gacgggcctc aagcgccgcg tggcccccga cggcctgccc gcgcgcgcga
     2401 tcgcgctcgc ctcgtcggct cgcgcgttcg tcgtctccgt ggacctgcct tcgggtctct
     2461 cggccgacga ggccgtgccc tgggagccgt ccgtcagggc cgatctcacc gtgacgttcg
     2521 gctggccgaa gccgtgccac gtggagcgcg ccccgggcgc gcgctgcggg cgcgtcgccg
     2581 tcgcggac
//
LOCUS       DAOMFY010000401         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_18365_length_2586_cov_6.557882, whole genome shotgun sequence.
ACCESSION   DAOMFY010000401 DAOMFY010000000
VERSION     DAOMFY010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18365_length_2586_cov_6.557882"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2586
                     /locus_tag="PLB01_19205"
     CDS             <1..>2586
                     /locus_tag="PLB01_19205"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79482.1"
                     /translation="RAIFGCTGVVLADASVGSDGVATWSARQGLGHVGSNGDVVLSGR
                     TTVQGDAIPGPGRRAVVPPESKVTGLTTPARDVADCRPVDLAAFEAFRSRNDNARIPK
                     TAGGRDAVKDGALVLDGPDPLTLPAGTYVFSSIALSGRAHVLFSGEAHVFVTGDVSLT
                     GNSHVNPAGGGFRLRLVSAGARVSLDTLSSLGAFVYAPGAAATFATGSRLVGSVFADS
                     VVVREASVAIRAVDDTPPVVDLVEPVEDAAVSLAAVRVRGSARDPETAVTAVVVNGVA
                     TPVAADGSFDVTVDARATLEVRADVMNAAGLTASLRRSLCTGAPTVAIRTPASGSVVA
                     SRIGSVVGTCGSASTVTVNGVPASVSGGAFRAEGVDFGPDGLATVTVIASNACASSAA
                     VSAFIVDSAAPVLAIDSPAPGSVFGASPISVTGTFVEANLVDITVNGVVAAITGNRFT
                     ATVPISPGSSTLAAVARDVAGRSGRSAEISVSLEAGTPNVRITSPASGTLTGTATATV
                     TGVAEVPNLAGVRVAGIAATLSGSSFSAAGVPLVEGDNRLVAEAVDASARTFSSAAVV
                     VTLDTLPPAVVLETAGLPVLTNLAAVAVSGTVSDPHLAGVTVNGVAAVVSGGRFTAPA
                     VPLAEGDNVLRARATDTLGHAADSNSATVNRDTQAPAVAITSPAPSAQLAASSVTVTG
                     TATDAHLAAVTVNGVAAAVANGQFTATLTLPEGDTALVARATDAAGNAGASAAVGVTV
                     DTLPPVVSIDAPADPLTGSPFVTVTGTVVEPHLLSLTVSGVPASVSGGRWVAANVPLV
                     EGTQQVRAVALDTFGHRAESAPVEYRLDSTPPVLTMDAPAAASAACRAPGAPVTVSGR
                     VYGRGA"
BASE COUNT          279 a         1004 c          944 g          359 t
ORIGIN      
        1 ccgggcgatc ttcggctgca ccggcgttgt cctcgccgat gcgtccgtcg gctcggacgg
       61 cgtggcgacg tggtcggcca ggcagggact cgggcacgtg ggctcgaacg gcgacgtcgt
      121 cctctcgggg cgcacgacgg tccagggcga cgcgatcccc gggcccggcc ggcgcgcggt
      181 cgtgccgccg gagtcgaagg tcaccggcct cacgacaccc gcccgcgacg tcgccgactg
      241 ccgcccggtc gacctcgccg ccttcgaggc gttccggtcg cgcaacgaca acgcgcggat
      301 cccgaagacc gcgggcggac gcgacgccgt gaaggacggc gcgctcgtcc tcgacgggcc
      361 ggacccgctc acgctgcccg cgggcacgta cgtcttctcc tcgatcgcgc tgtcgggccg
      421 ggcgcacgtc ctcttctcgg gcgaggcgca cgtcttcgtc acgggcgacg tctcgctcac
      481 gggcaacagc cacgtgaatc ccgccggcgg cggcttccgc ctccggctcg tttcggccgg
      541 agcgcgcgtc agcctcgaca cgctctcgtc gctcggcgcg ttcgtgtacg cgccgggcgc
      601 ggccgcgacg ttcgccacgg gcagccggct ggtcggctcg gtcttcgcgg actcggtcgt
      661 cgtgcgcgag gcgtctgtcg cgatccgggc cgtggacgac acgcctcccg tcgtcgacct
      721 cgtcgagccg gtggaagacg cggccgtctc gctggccgcc gtgcgcgtgc gcggcagcgc
      781 gcgggatccc gagacggcgg tgacggcggt cgtcgtgaac ggcgtcgcca cgcccgtcgc
      841 cgccgacggc tcgttcgacg tgacggtcga cgcaagggcc acgctcgagg tccgggcgga
      901 cgtcatgaat gcggccggcc tcacggcgag cctccggcgg tccctctgca cgggagcgcc
      961 gacggtggcg atccggactc cggcgtccgg ctcggtcgtc gcctcgcgca tcggctccgt
     1021 cgtcgggacg tgcgggtccg cctcgaccgt caccgtgaac ggcgtccccg cctccgtctc
     1081 cggcggagcg ttccgggcgg agggcgtcga cttcggtccg gacggcctcg cgaccgtaac
     1141 ggtcatcgcg tccaacgcgt gcgccagctc ggcggcggtc tcggcgttca tcgtcgactc
     1201 cgcggcgccc gtcctcgcga tcgacagccc cgcgcccggc tcggtcttcg gcgcctcgcc
     1261 gatctccgtc acgggcacgt tcgtcgaggc gaacctcgtc gacatcaccg tcaacggcgt
     1321 cgtggcggcg atcacgggca accgcttcac ggccacggtc ccgatttcgc cgggttcctc
     1381 gacgctcgcc gccgtcgcgc gggacgtcgc cgggcgttcg ggccgctccg cggagatctc
     1441 ggtctcgctc gaggctggca cgccgaacgt gagaatcacg agccccgcct cggggacgct
     1501 caccgggacc gcgacggcga cggtcacggg cgtcgccgag gttccgaacc tcgccggcgt
     1561 ccgggtggcg gggatcgcgg cgacgctctc gggctcgtcc ttcagcgccg ccggcgtgcc
     1621 cctcgtcgag ggagacaacc gcctcgtcgc cgaggccgtg gacgcgtcgg cccggacgtt
     1681 ctcgtcggca gcggtcgtcg tcacgctcga caccctgccg ccggccgtcg tgctcgagac
     1741 cgccgggctg cccgtgctga cgaacctcgc ggccgtcgcc gtctcgggca ccgtgtcgga
     1801 tccgcacctc gcgggcgtga ccgtgaacgg cgtcgcggcg gtcgtgtcgg gggggcgctt
     1861 cacggcgccc gccgtgccgc tcgccgaggg agacaacgtc ctccgcgcgc gcgcaacgga
     1921 cacgctcggc cacgcggcgg actcgaactc cgccacggtg aaccgcgaca cgcaggcgcc
     1981 ggccgtggcg atcacgtcgc ctgcgccgag cgcgcagctc gcggcgtcga gcgtcacggt
     2041 cacgggcacc gccacggacg cgcatcttgc ggccgtcacc gtgaacgggg tcgcggcggc
     2101 cgttgcgaac gggcagttca cggcgacgct gacgctgccc gagggcgaca cggcgctcgt
     2161 ggcgcgcgcg accgacgccg ccgggaacgc cggcgcctcg gcggccgtcg gcgtgaccgt
     2221 ggacacgctc cccccggtcg tctcgatcga cgccccggcc gacccgctca cgggttcccc
     2281 cttcgtcacc gtgacgggca cggtcgtcga gccgcacctc ctgtcgctca cggtctccgg
     2341 cgtgcccgcg agcgtctcgg gcggcaggtg ggtcgccgca aacgtcccgc tcgtcgaggg
     2401 cacgcagcag gtcagggccg tcgcgctcga tacgttcggc caccgcgccg aatcggctcc
     2461 cgtggaatac cggctcgact cgacgccgcc cgtcctcacg atggacgcgc ccgccgcggc
     2521 ctcggccgcc tgtcgcgcgc ccggggcgcc ggtgacggtc tcgggccgcg tctacgggcg
     2581 gggcgc
//
LOCUS       DAOMFY010000402         2577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_18482_length_2577_cov_7.799366, whole genome shotgun sequence.
ACCESSION   DAOMFY010000402 DAOMFY010000000
VERSION     DAOMFY010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18482_length_2577_cov_7.799366"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /gene="pyrF"
                     /locus_tag="PLB01_19210"
     CDS             complement(<1..460)
                     /gene="pyrF"
                     /locus_tag="PLB01_19210"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQQ79483.1"
                     /translation="MPDARGRLAVALDLPDRAALLAMARRVAPEAGVLKIGLEAFVAE
                     GPVLVREVGASGADVFLDLKLHDIPNTVSRAAAAAVATGAAIVNCHASGGRDMMRGFG
                     DGGRAAAAKASRPVPKLIAVTVLTSLDAPSLAAVGLAGTPREAAVRLASLA"
     gene            complement(453..1400)
                     /locus_tag="PLB01_19215"
     CDS             complement(453..1400)
                     /locus_tag="PLB01_19215"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HQQ79484.1"
                     /translation="MMAVDLTVQMGRLRLKNPIATASGTFGYGLEFADYLDLSALGAI
                     SVKGLSRRPCHGNPPPRICETDAGMLNAIGLQNVGVDAFLAEKLPELHAKGATVIANV
                     WGDAEEDYVAVVERIGADRRVAAIELNVSCPNVVKGGMIFGNNPQALSTLVKKVRAAT
                     PHVLVVKLSPNVTDIVAVAQAAADAGADALSLINTLVGLAIDLETRTPKIGFTTGGLS
                     GPAIRPVALRMVWEVRKALPKIPIFGMGGIETVENVAEFLVAGANAVQIGTANFRDPS
                     LAGRLVGELEAWCAERKIARVSDLVGTLKTRPREDHRFA"
     gene            complement(1397..2203)
                     /locus_tag="PLB01_19220"
     CDS             complement(1397..2203)
                     /locus_tag="PLB01_19220"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HQQ79485.1"
                     /translation="MAVNETLRVLRTLHYDAAHYSLFLSAGTIGASAKPGHFAMLQAG
                     DGLRPYLRRAFSIADVTTVAGVPAIEFVVKAVGVGTQALGRLPEGTPVPVLGPLGVPF
                     PTDDLKPGDRVALVAGGIGLAPLVLLARTLSARGLAADLFYGGRNENDVLKRADFERF
                     LGPHRCRYATDDGSLGRRGRVTDILAHALEGGTSYRRVFACGPLPMFRTLAALVEKAS
                     LDASFAMESEMACGFGVCLGCVAPTADGRFATICKEGPCVPPSFIDWKKI"
     gene            2260..>2577
                     /locus_tag="PLB01_19225"
     CDS             2260..>2577
                     /locus_tag="PLB01_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79486.1"
                     /translation="MPFVLAGALAGVTAADVLIGLNPDLLAPAPALRALGVLGAIGAA
                     LFAPLALLRKRPATRASTLLLALLLGLAAAGVEVQRRAAHDFVPGGGRRVLAAAALAL
                     AGGA"
BASE COUNT          353 a          897 c          986 g          341 t
ORIGIN      
        1 gcgcgaggga cgcgaggcgc acggcggcct cgcgcggcgt tccggcgagg ccgacggccg
       61 cgagcgacgg cgcgtcgagg gacgtgagga ccgtgacggc gatgagcttg ggcaccgggc
      121 ggctcgcctt cgccgccgcg gcgcggcctc cgtcgccgaa gccccgcatc atgtcgcgcc
      181 cgccggacgc gtggcagttc acgatcgcgg cgcccgtcgc gaccgccgcg gccgccgcac
      241 gggagacggt gttcggaatg tcgtggagct tgaggtcgag gaagacgtcc gctcccgacg
      301 cgcccacctc gcggaccagc accggccctt ccgcgacgaa ggcctcgaga ccgatcttca
      361 ggacgcccgc ctcgggggcg acgcggcgcg ccatcgcgag gagcgcggcg cggtcgggca
      421 ggtcgagggc gacggcgagc cggccgcgcg cgtcaggcaa agcggtggtc ctcgcgcggg
      481 cgggtcttca gggtcccgac gaggtccgag acgcgcgcga tcttccgctc cgcgcaccag
      541 gcctcgagct cgccgacgag ccgcccggcg agcgagggat cgcggaagtt cgcggtgccg
      601 atctggacag cgttcgcgcc ggcgacgagg aactcggcga cgttctcgac ggtctcgatc
      661 ccgcccatgc cgaagatcgg gatcttcggg agtgccttgc gcacctccca gaccatccgc
      721 agggcgaccg gccggatggc cgggccggag aggccgcccg tcgtgaaccc gatcttcggc
      781 gtgcgcgtct cgagatcgat cgcgaggccg acgagcgtgt tgatgaggga gagcgcgtcg
      841 gcgccggcgt ccgccgccgc ctgcgcgacc gcgacgatgt ccgtcacgtt cggcgagagc
      901 ttcacgacga ggacgtgcgg cgtggcggcc cggaccttct tcacgagggt cgagagcgcc
      961 tgggggttgt tcccgaagat catgccgccc ttcacgacgt tcgggcacga cacgttgagc
     1021 tcgatcgccg cgacccggcg gtccgcgccg atccgctcga cgacggcgac gtagtcctcc
     1081 tcggcgtccc cccacacgtt cgcgatcacg gtcgcgccct tcgcgtggag ttcggggagc
     1141 ttctcggcga ggaacgcgtc cacgccgacg ttctgcaggc cgatcgcgtt cagcattccc
     1201 gcgtccgtct cgcagatgcg cggcggcggg ttgccgtggc acggccggcg cgacagcccc
     1261 ttgacggaga tcgcgccgag cgccgagagg tcgaggtagt cggcgaactc gaggccgtag
     1321 ccgaaggtgc ccgaggccgt cgcgatcggg ttcttcaggc gcaggcgccc catctggacg
     1381 gtgaggtcga cggccatcag atcttcttcc agtcgatgaa ggacggcggc acgcacggac
     1441 cttccttgca gatcgtcgcg aagcggccgt ccgcggtggg cgcgacgcag ccgaggcaga
     1501 cgccgaagcc gcacgccatc tcggactcca tcgcgaagga ggcgtcgagg ctcgccttct
     1561 cgacgagagc ggcgagcgtc ctgaacatcg ggagcgggcc gcacgcgaag acgcgccggt
     1621 aggaagttcc gccttccaga gcgtgcgcga ggatgtccgt gacgcgcccc cgccggccga
     1681 gggagccgtc gtcggtggcg taccggcagc ggtgagggcc gaggaagcgc tcgaagtccg
     1741 cgcgcttcag gacgtcgttc tcgttgcggc cgccatagaa gaggtccgcg gcgaggccgc
     1801 gggctgagag ggtgcgcgcg agaaggacga gcggcgcgag gccgatcccg cccgcgacga
     1861 gggcgacgcg gtcaccgggc ttgaggtcgt ccgtcgggaa gggaacgccg agcgggccga
     1921 gcacgggcac cggcgtgccc tcggggagcc tcccgagcgc ctgcgtgccg acgccgacgg
     1981 ccttcacgac gaactcgatc gcgggaacgc cggcgaccgt cgtgacgtcc gcgatcgaga
     2041 acgcgcgccg gaggtacggg cggaggccgt cgccggcctg gagcatcgcg aagtgacccg
     2101 gcttcgcgga cgcgccgatc gtccccgccg agaggaacag cgaatagtgc gccgcgtcgt
     2161 agtgcagcgt gcggaggacg cgaagcgttt cgttgacggc catcgggaag ggggcgagac
     2221 tcgccgggcg cggattatat ggacgagaga cgtcgggcgt tgccgttcgt tctggcgggg
     2281 gcgctcgcgg gcgtcacggc ggccgacgtc ctgatcggat tgaatcccga tctcctcgcg
     2341 cccgcccccg cgttgcgcgc cctgggcgtg ctcggggcga tcggcgcggc ccttttcgcg
     2401 ccgctcgcgt tgctccggaa gcggcccgcg acgcgggcgt cgacgctcct tctcgccctc
     2461 cttctcggcc tcgcggcggc cggcgtcgaa gtccagcgcc gggccgcgca cgacttcgtt
     2521 cccggaggcg gacgccgcgt cctcgcggcg gcggcgctcg cgctcgcggg aggcgcg
//
LOCUS       DAOMFY010000403         2560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_18696_length_2560_cov_4.776048, whole genome shotgun sequence.
ACCESSION   DAOMFY010000403 DAOMFY010000000
VERSION     DAOMFY010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18696_length_2560_cov_4.776048"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLB01_19230"
     CDS             complement(<1..191)
                     /locus_tag="PLB01_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HQQ79487.1"
                     /translation="MTEDAAGLLDGPFSALAEAVSSGRVAARGVAEASLARAVKARDL
                     YGAFLSIDEAAALRQAEAV"
     gene            complement(188..478)
                     /gene="gatC"
                     /locus_tag="PLB01_19235"
     CDS             complement(188..478)
                     /gene="gatC"
                     /locus_tag="PLB01_19235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQQ79488.1"
                     /translation="MKIDAALVSRVASLAALELTDAERDALSGQLTRIVEHFEALRAV
                     PDELLAEEPASPPSPLRADEAKGGSPGALVETNAPEFAHGHFVVPRAVSRDA"
     gene            545..2548
                     /locus_tag="PLB01_19240"
     CDS             545..2548
                     /locus_tag="PLB01_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HQQ79489.1"
                     /translation="MRPFLLFRPGGGVPESVRAPLERAGSDVLVAGNVEEAVSLAASS
                     QPEVVLVVPPGTVSEAAALLSRLPESGVRPPIFVLEDEGAPHRPAALETIARGPWSRA
                     TAALGSGSDDPAPALLLSRVQLTGLLDLWEQVAGGASGFVEHALRSLALALDASRVTL
                     YRWKAGSAEAAVEGSSLGPGVVGRTVEIVRYPELRAAAARSGAVLVEEIERDPLMEAA
                     SPFLSGQANRSLLCQLVPGDGSDLYLHAVREGTPFGLVDVALLAATARILHASLAVTR
                     PPEPLEKGPDRKRMRTVQRILTGIPDATAMVGPSGEILLVNPPFCSMTRRLDSELVGL
                     DIRSLLRPLAPEDAFTTLTPRPGTPVTTTRARLVTAAGETIPVEVLSIPTPDETAGKA
                     GWSTITLRDRRHEDAREAREQALQRDADETGRQLEALQQSVRESDVVRARFWTAAAHE
                     LRTPLAIAQSYLEVVLSDLAEDLPERPTQLLKTASESLTRLERLIADVLDAAVSGRAR
                     STLRLAELDLAEVLRGLAPALSAAAFKRGVRLSVEIPKGLPKVPGDREKVERLVTNLV
                     DHSLRRVPRGGPDVRLLASASDGRAFVRVVDRAPTLTPEKAAHLYDDVGSARGAGEFG
                     LSVARRLADAMAAELSAHAEPDGSNVKSVAWTLPAPDAAGPDA"
BASE COUNT          305 a         1022 c          906 g          327 t
ORIGIN      
        1 tccacggcct ccgcctggcg cagggcggcg gcctcgtcga tcgagaggaa cgccccgtag
       61 aggtcgcgcg ccttcacggc gcgcgcgagg gacgcttcgg ccacgccccg cgccgccacg
      121 cgtcccgacg acacggcctc cgcgagcgcg gagaacgggc cgtccaggag cccggcggcg
      181 tcctccgtca cgcgtccctc gagacggccc gggggacgac gaagtggccg tgggcgaact
      241 cgggcgcgtt cgtctccacg agcgcgcccg gcgaaccgcc cttcgcctcg tcggcccgga
      301 ggggcgacgg cggagacgcg ggctcctccg cgaggagctc gtcgggaacg gcgcggaggg
      361 cctcgaagtg ctcgacgatg cgcgtgagct gcccggagag ggcgtcgcgc tcggcgtcgg
      421 tcagctcgag cgcggcgagg gacgccacgc gcgacacgag cgccgcgtcg atcttcactt
      481 gcaaattcta gcagccgcct tgacgcgggc gacgcccgcc cgtaccgtgc tttggagtcg
      541 atccatgcgg cccttcctcc tgttccgtcc gggaggcggc gtcccggaat ccgtgcgcgc
      601 gccgctcgag cgcgcgggca gcgacgtgct cgtcgcgggc aacgtcgaag aagcggtgtc
      661 gctcgccgcg tcctcgcagc ccgaggtcgt cctcgtcgtc ccgcccggca ccgtctcgga
      721 ggcggccgcg ctcctctcgc gccttcccga gtcgggcgtt cggccgccga tcttcgtcct
      781 cgaggacgaa ggcgcccccc accgtcccgc cgcgctcgag acgatcgcgc gcggcccgtg
      841 gagccgcgcg accgccgcac tcggcagcgg ctccgacgac ccggcgccgg cgctcctcct
      901 ctcgcgtgtc cagctcaccg gcctcctcga cctctgggag caggtcgcgg ggggcgcttc
      961 cggcttcgtc gagcacgcgc ttcgctccct cgcgctcgcg ctcgacgcct cccgcgtgac
     1021 cctgtatcgc tggaaggccg gctccgccga ggccgcggtc gagggatcca gcctcggccc
     1081 cggagtcgtc gggcggacgg tcgagatcgt ccgttacccc gagctccgcg cggccgccgc
     1141 ccggtcgggc gcggtcctcg tcgaggagat cgaacgggat ccgctgatgg aggccgccag
     1201 cccgttcctc tccgggcagg cgaaccgctc cctcctctgc cagctcgtcc ccggcgacgg
     1261 gagcgacctc tacctgcacg cggtgcgcga ggggacgccg ttcggcctcg tcgacgtcgc
     1321 gcttctcgcc gcgaccgcgc gaatcctgca cgcgtcgctc gccgtcacgc gcccccccga
     1381 accgctcgag aaggggcccg accgcaagcg gatgcggacg gtccagcgga tcctcacggg
     1441 catcccggac gcgacggcga tggtcggccc gtcgggcgag atcctgctcg tgaaccctcc
     1501 tttctgctcg atgacgcgcc ggctggactc cgagctcgtg gggctcgaca tccgctcgct
     1561 gctccgtccg ctcgcgccgg aggacgcctt cacgacgctg acgcctcggc ccggcacgcc
     1621 ggtcacgacg acgcgcgcgc gcctcgtgac ggccgcgggc gagacgatcc ccgtcgaggt
     1681 cctttcgatc cccacgcccg acgagaccgc cggcaaggcc gggtggtcga cgatcacgct
     1741 ccgcgaccgg cgccacgagg acgcccgcga ggcgcgcgag caggcgctcc agcgcgacgc
     1801 cgacgagacg ggccgccagc tcgaggccct gcagcagagt gtgcgggagt ccgacgtcgt
     1861 gcgcgcgcgg ttctggaccg ccgcggccca cgagctgcgc accccgctcg cgatcgcgca
     1921 gagctacctc gaagtcgtcc tgagcgacct cgccgaagac ctgccggagc gcccgaccca
     1981 gctcctcaag accgcgtccg agagcctcac gcgcctcgag cgcctcatcg ccgacgtcct
     2041 cgacgcggcc gtcagcgggc gcgcgcgctc gacactccgg ctcgccgagc tggacctcgc
     2101 cgaggtcctg cgcggcctcg cgcccgcgct ctcggccgcg gcgttcaagc gcggcgtgcg
     2161 cctctcggtc gagatcccga aggggctccc gaaggtcccg ggcgaccgcg agaaggtcga
     2221 gcggctcgtc acgaacctcg tcgaccactc gctccgccgc gtgccgcgcg gcggccccga
     2281 cgtccggctc ctcgcgtccg cgtccgacgg ccgggcgttc gtccgggtcg tcgaccgtgc
     2341 gcccacgctc acgcccgaga aggcggccca cctctacgac gacgtcggct ccgcgcgcgg
     2401 cgccggcgag ttcgggctgt cggtcgcacg ccggctcgcg gacgcgatgg ccgcggagct
     2461 ctcggcgcac gcggagcccg acggctcgaa cgtgaagagc gtcgcctgga cgctcccggc
     2521 ccccgacgcg gccgggccgg acgcgtgacg gacggttccg
//
LOCUS       DAOMFY010000404         2555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_18769_length_2555_cov_6.181600, whole genome shotgun sequence.
ACCESSION   DAOMFY010000404 DAOMFY010000000
VERSION     DAOMFY010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18769_length_2555_cov_6.181600"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLB01_19245"
     CDS             <1..271
                     /locus_tag="PLB01_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79490.1"
                     /translation="TDLTAAGERALTDARVAAVKRVEDSAIGRYAAPLTKRLDYRVYR
                     AFVAGRARATAYSQLVFERLFGGSVETVQSAAAPAPEPSPRIRRS"
     gene            288..875
                     /locus_tag="PLB01_19250"
     CDS             288..875
                     /locus_tag="PLB01_19250"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HQQ79491.1"
                     /translation="MNDTSQTPQAPPAAFPASAPPPPPPVAFGPAAPAQATLPPGVKN
                     PGAAGILGIIPGLGHLYLGLMQRGAVLFGVWVLLITVASHGQARGPFPGILIPFWMIF
                     SIIDAVQQAKAINATGSPVPGILGEDRPVKVNGSLTAGILLVLVGGFLLLSNFVTIDL
                     SFLVDWWPALLVLFGAWQIFSYWKGKQDETKNEAG"
     gene            complement(872..1396)
                     /locus_tag="PLB01_19255"
     CDS             complement(872..1396)
                     /locus_tag="PLB01_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79492.1"
                     /translation="MTPLLPFLLFLAASPVAGVPLPPDGAVDQVFTEIYASRAAAETK
                     AALAQLDADTSRSSAEKKQIKKSLEKLKVVVLTTPKTLDEAVAFYEREVKGARFIFAV
                     RNLEADLKDGMRSGAIKGDPSVVAKAAGQQSRSARWNRADGALEIAIEDRILDPRTGE
                     ILAKTIVLVTSLGD"
     gene            complement(1406..2329)
                     /locus_tag="PLB01_19260"
     CDS             complement(1406..2329)
                     /locus_tag="PLB01_19260"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQQ79493.1"
                     /translation="MTTGRDLFAPKLGLTLPLCGLHFIFFFLRTADPERTLSLFAFRP
                     GVALTQPWTFVTYQFLNAGPMDLFFGTLILYILGSALEAEWGTGEFTAFWLVATLGGS
                     LAALVTGNALASGGALTGISMLFAYAWLFPDMEFLMFFVIPVKVKWLAWLALAFLLWD
                     FGKLVLGGHFGLGLVHVAGSAAGFLFFWVRHHGRFKARRAAQSAVVAVKTAGAVRADV
                     ALERRNRDLFPRVEELRRARQGGDPPPRTLEFEEELRKLVVPGVNICKPVDFKGDRDG
                     ICVTCEGFAECSLRYVNGQPEQIVVKERAGS"
     gene            complement(2326..>2555)
                     /locus_tag="PLB01_19265"
     CDS             complement(2326..>2555)
                     /locus_tag="PLB01_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HQQ79494.1"
                     /translation="PDGSAAAPALRREALAASLACRGAITINHRLAPAEAARLLADLA
                     DCRDPWTCPHGRPIVLSFVHGEIEKRFGRRG"
BASE COUNT          395 a          922 c          863 g          375 t
ORIGIN      
        1 gaccgacttg acggccgcgg gcgagcgcgc gctcaccgac gcgcgcgtcg ccgcggtgaa
       61 gcgtgtcgag gacagcgcga tcgggcgcta cgcggcgccg ctcacgaagc gcctcgacta
      121 ccgcgtctac cgcgcgttcg tcgcgggccg cgcccgcgcg acggcgtatt cccagctcgt
      181 cttcgagagg ttgttcggcg gctccgtcga gaccgtgcag tccgcggccg cgcccgcgcc
      241 ggaaccttct ccacgcatcc gacgttcctg aaggcaaggt gagaacgatg aacgacacga
      301 gccagacccc ccaagcccct cccgcggcat tccccgcctc ggcgccgcct cccccgccgc
      361 cggtcgcatt cggcccggcc gcgccggccc aggcgacgct tccgcccggc gtgaagaacc
      421 ccggcgccgc ggggattctc gggatcattc cgggcctcgg ccacctctac ctcggcctca
      481 tgcagcgcgg cgctgtgctg ttcggcgtct gggtgctcct gatcacggtc gcgagccacg
      541 gccaggcgcg cggcccgttc cccgggatcc tgattccctt ctggatgatc ttctcgatca
      601 tcgacgccgt gcagcaggcc aaggcgatca acgccaccgg ctcgcccgtg cccgggatcc
      661 tcggcgagga caggcccgtc aaggtgaacg gcagcctcac ggccggcatc ctcctcgtcc
      721 tggtcggcgg gttcctgctc ctcagcaact tcgtgacgat cgacctgtcg ttcctcgtcg
      781 actggtggcc cgcgctcctc gtcctcttcg gtgcgtggca gatcttcagc tactggaagg
      841 gcaagcagga cgagacgaag aacgaagccg gctagtcccc gagggacgtc acgaggacga
      901 tcgtcttcgc gaggatctcc ccggtccgcg ggtcgaggat gcggtcctcg atcgcgatct
      961 cgagggcccc gtcggcgcgg ttccaccgcg ccgaacggct ttgctggccc gccgccttcg
     1021 cgacgaccga cgggtcgccc ttgatcgcgc cggagcgcat cccgtccttc aggtcggcct
     1081 cgaggtttct cacggcgaag atgaaccggg cgcccttcac ctcccgctcg tagaacgcga
     1141 ccgcctcgtc gagcgtcttc ggcgtcgtca ggacgacgac cttcagcttc tcgaggctct
     1201 ttttgatctg cttcttctcc gcgctcgagc gcgacgtgtc cgcgtcgagc tgggcgaggg
     1261 cggccttcgt ctcggcggcc gcgcggctcg cgtagatctc cgtgaagacc tggtcgacgg
     1321 cgccgtccgg aggaagcggc acgccggcga ccggcgacgc ggcgaggaag aggaggaacg
     1381 ggaggagcgg cgtcatgtcc ttattctagg atcctgcccg ttccttcacg acgatctgct
     1441 cgggctggcc gttcacgtag cgcagggagc attccgcaaa gccctcgcac gtgacgcaga
     1501 tgccgtcccg gtcgcccttg aagtcgaccg gcttgcagat gttcacgccg gggacgacga
     1561 gcttccggag ctcctcctcg aactcgagcg tgcgcggcgg cgggtcgccg ccctgtcgcg
     1621 cgcggcgcag ctcctcgacg cgcgggaaga ggtcgcggtt gcgcctctcg agcgccacgt
     1681 ccgcgcgcac cgctcccgcg gtcttcacgg cgacgaccgc ggactgcgcg gcccttcgcg
     1741 ccttgaaccg gccgtggtgg cggacccaga agaagaggaa tccggcggcg gaccctgcga
     1801 cgtggacgag gccaaggccg aagtggcctc caaggacgag cttcccgaaa tcccacagga
     1861 gaaacgcgag cgcgagccac gcgagccatt tcaccttcac ggggatcacg aagaacatga
     1921 ggaactccat gtccgggaag agccacgcat acgcgaagag catcgagatg cccgtcaggg
     1981 ctccgcccga cgcgagagcg ttcccggtca cgagcgccgc gagcgagccg ccgagcgtcg
     2041 cgacgagcca gaacgccgtg aactccccgg tcccccactc cgcctccagc gccgacccga
     2101 ggatgtagag gatcagcgtc ccgaagaaca ggtccatcgg gccggcgttc aggaactggt
     2161 acgtgacgaa cgtccagggc tgcgtgaggg caaccccggg ccggaacgcg aagagcgaca
     2221 gcgtgcgctc ggggtccgcg gtcctcagga agaagaagat gaagtgcagg ccgcagagcg
     2281 ggagcgtgag cccgagcttc ggcgcgaaaa ggtcccgtcc ggtcgtcatc cacgcctccc
     2341 gaaccgcttc tcgatctcgc cgtgcacgaa cgacagcacg atcggcctcc cgtgcggaca
     2401 ggtccacggg tcgcggcagt ccgcgaggtc cgcgagaagg cgcgccgcct cggccggcgc
     2461 gagccggtgg ttgatcgtga tcgcgccccg gcacgcgagg gacgccgcga gcgcctcgcg
     2521 ccgaagggcg ggcgccgccg cggatccgtc cggca
//
LOCUS       DAOMFY010000405         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_18861_length_2549_cov_3.641540, whole genome shotgun sequence.
ACCESSION   DAOMFY010000405 DAOMFY010000000
VERSION     DAOMFY010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18861_length_2549_cov_3.641540"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLB01_19270"
     CDS             complement(<1..592)
                     /locus_tag="PLB01_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79495.1"
                     /translation="MPPARFPGLPLLKAFEGALKEGLLLDEALGGILNAALHFFDAPV
                     VALLPGAGVPPMTRSGRSSVAAAAETRLNQHLSEVLAQGRAKKASEGGLSFFGAPVKV
                     KDQTQAVFGVVLETIAGTDAEAEEAAREFARTIGHVLERDRTLGTLMKRREEAVALFE
                     LASGAFLSLNPDEVIRLTVASLSRELEFDRVSAFRFQ"
     gene            633..>1886
                     /locus_tag="PLB01_19275"
     CDS             633..>1886
                     /locus_tag="PLB01_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQQ79496.1"
                     /translation="MLRAKPNPIAPAEPTAATESASTDSLAESLAEILPALERFTRFE
                     GPDGAAKRTAWRRALDGPLPEEGEGREAVLAALRDVVIPNGLRIGAPGFTGWVTTSPT
                     TVPAVAQLAGSITAAQRWWATPGNFLEVLALRWVARLVGLPDTFEGSFTSGGSTANLL
                     GLAAARQHAGERLGVDVGLEGLAALPSPRVYASTEVHHVVHRACGVLGLGRKALRLVP
                     MDAARRVDLGALERLIDTDVRAGATPVAVVSSAGDVNTGVVEPIDAMREIAHARGVWL
                     HADGAYGGFGVLDPRVAPLYGDLTKIDSFAVDPHKWMAAPVGCGAAFVRDGGFLARAL
                     TLEPAAYVEMAPTDTGDLGSPFDQRGEGNIDTSLDHSAPARGLAVWALLKEIGAKGMR
                     ERVVRHHNCARRVAERVRAHPALELM"
     assembly_gap    1887..1896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1897..2140
                     /locus_tag="PLB01_19280"
     CDS             <1897..2140
                     /locus_tag="PLB01_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79497.1"
                     /translation="LSICCFRVKPAGVADEAALEALNEKVLLAVRAKGRAVPSHTRID
                     GRFAIRPCFINPRQGLAEADLTVDEVLAAARELGAI"
     gene            complement(2137..>2549)
                     /locus_tag="PLB01_19285"
     CDS             complement(2137..>2549)
                     /locus_tag="PLB01_19285"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HQQ79498.1"
                     /translation="LGVFFVAAALPKVVDPPGFAHMIYNYRIVPGAFVNLMALVLPWF
                     ELLAGLALILGIWTRTSAAAIGALLLVFIVAISFNLARGNAIDCGCFDVAAAGKTVAE
                     RITDMWWVVIRDVGMLVMVAQILWATGRVEPASA"
BASE COUNT          366 a          905 c          938 g          330 t
ORIGIN      
        1 gctggaaccg gaacgcgctc acgcggtcga actcgagctc gcgcgagagc gacgcgaccg
       61 tgaggcggat gacctcgtcc ggattcaggg agaggaacgc ccccgacgcc agctcgaaga
      121 gcgcgacggc ttcctcgcgg cgcttcatga gcgtcccgag cgtgcggtcg cgctcgagaa
      181 cgtgtccgat cgtccgcgcg aattcccgcg cggcctcctc ggcctcggcg tccgttccgg
      241 cgatcgtctc gaggacgacg ccgaagacgg cctgcgtctg gtccttgacc ttcacgggcg
      301 cgccgaagaa cgagagcccg ccctcggacg ccttcttggc gcggccctgg gcgaggacct
      361 cggagaggtg ctggttgagc cgcgtctcgg ctgcggcggc cacgctggag cgtcccgagc
      421 gcgtcatcgg cgggacgccc gctccgggaa gaagcgcgac gaccggcgcg tcgaagaagt
      481 gcaaggccgc gttcaggatt ccgcccagag cctcgtcgag gagcagaccc tccttgagcg
      541 ctccttcgaa cgccttcagg agagggaggc cgggaaagcg ggcgggcggc atggcggcga
      601 acggccataa taccgcctcc ccggaggtgc ccatgctccg cgcaaagccg aatccgatcg
      661 ctccggcgga gccgaccgcc gcgaccgagt ccgcgtccac ggactcgctc gccgaatcgc
      721 tggccgaaat cctcccggcg ctcgagcgct tcacgcgttt cgagggaccg gacggcgcgg
      781 cgaagcggac ggcgtggcgc cgcgcgctgg acggcccgct gcccgaggag ggcgaggggc
      841 gcgaggcggt cctggccgcg ctgagggacg tcgtgatccc gaacggcctg agaatcgggg
      901 cgccgggctt cacgggctgg gtcacgacct cgccgacgac ggtgcccgcg gtcgcgcagc
      961 tcgcgggctc gatcaccgcg gcccagcgct ggtgggcgac gcccgggaac ttcctcgagg
     1021 tcctcgcgct gcgctgggtg gcgcgcctcg tgggccttcc cgacacgttc gagggctcgt
     1081 tcacgagcgg cggctcgacc gcgaacctcc tcggcctcgc cgcggcgcgc cagcacgccg
     1141 gcgagcgcct cggcgtcgac gtcgggctcg agggcctcgc cgcgctcccg tcgccgcggg
     1201 tgtacgcgtc cacggaagtc caccacgtcg tccacagggc ctgcggcgtc ctcggcctcg
     1261 gccgcaaggc gctgcgcctc gtcccgatgg acgcggcgcg gcgcgtggac ctcggggcgc
     1321 tcgaacggct catcgacacc gacgtccgcg ccggagcgac gcccgtcgcc gtcgtttcgt
     1381 ccgcgggcga cgtgaacacg ggcgtcgtgg agccgatcga cgcgatgcgc gaaatcgcgc
     1441 acgcgcgcgg cgtctggctg cacgcggacg gggcgtacgg gggcttcggc gtcctcgacc
     1501 cgcgcgtggc gccgctctac ggcgacctga cgaagatcga ttccttcgcc gtcgacccgc
     1561 acaagtggat ggcggcgccg gtcggctgcg gggcggcctt cgtccgcgac gggggttttc
     1621 tcgctcgggc cctcacgctc gagcccgcgg cgtacgtcga gatggccccg acggacacgg
     1681 gagatctcgg ctcgccgttc gaccagaggg gggagggaaa catcgacacc tcgctcgacc
     1741 actcggcacc ggcgcgcggc ctcgccgtgt gggcgctcct gaaggagatc ggggcgaagg
     1801 gcatgcgcga gcgcgtcgtc cgccatcaca actgcgcgcg ccgcgtcgcc gagcgcgtgc
     1861 gcgcgcaccc ggcgctcgag ctcatgnnnn nnnnnncctc tcgatctgct gcttccgcgt
     1921 gaagcccgcg ggcgtggcgg acgaagccgc gctcgaggcg ctgaacgaga aggtcctcct
     1981 cgccgtgcgg gcgaagggcc gcgcggtccc gtcgcacacg cgcatcgacg gccgattcgc
     2041 gatccgcccc tgcttcatca acccgcggca ggggctcgcg gaggcggact tgaccgtgga
     2101 cgaggtgctg gcggcggcga gggagctggg cgcgatctag gccgacgcgg gctcgacccg
     2161 tcccgtcgcc cacaggatct gcgcgaccat cacgagcatt ccgacgtcgc ggatgacgac
     2221 ccaccacatg tccgtgatgc gttcggcgac ggtcttgccg gcggcggcga cgtcgaagca
     2281 gccgcagtcg atcgcgttgc cgcgcgcgag gttgaacgag atcgcgacga tgaaaacgag
     2341 aagcagcgcg ccgatcgccg cggccgacgt ccgcgtccag atcccgagga tgagcgcgag
     2401 gcccgccagg agctcgaacc acggcaggac gagcgccatg aggttcacga acgcgcccgg
     2461 cacgatccgg tagttgtaga tcatgtgggc gaagcccggc gggtcgacga ccttcggcag
     2521 cgccgcggcg acgaagaaga cgccgagcg
//
LOCUS       DAOMFY010000406         2547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_18897_length_2547_cov_7.935795, whole genome shotgun sequence.
ACCESSION   DAOMFY010000406 DAOMFY010000000
VERSION     DAOMFY010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18897_length_2547_cov_7.935795"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PLB01_19290"
     CDS             <1..1122
                     /locus_tag="PLB01_19290"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQQ79499.1"
                     /translation="AAETAAAPEKPAASLGVEERKRELAELRRRLAELKNRFESSKKR
                     EADLKGQLETADLDLQIQTGERRVLDLRKAEAEREATRAAADRDVAQREAEARRSDLG
                     TRMAALYRLGRLGYLRPLAAADSAGSFLSGLRLLTHLARRDASLLARHEEAAATLAAR
                     EEELAVRKRDLADLLAESRKKEVAFAAARARKAALLAQVHETARVEQKQVSTLEDKSE
                     RLAALLDLLEGRGRALAPGAASIRKYRGALDWPLKGTVVVPFGRIANPKFPKTFLRSS
                     GWTIGAPTGTPVRTTFSGEVVYAQWLKGYGNLVVVDHGDGVFTLYGRLATGTIGRGER
                     VSIGDRVGILGESPEDEPAGLYFEIRDNRTSADPATWLR"
     gene            1141..2448
                     /locus_tag="PLB01_19295"
     CDS             1141..2448
                     /locus_tag="PLB01_19295"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQQ79500.1"
                     /translation="MSKSRVAGLVASASLLLALFVVLPGMGADEKAQEKNDTLYRPLG
                     LFTEVLGLVRSNYVEPVELKPLLSGAFSGMTEALDPFSEYVPPDKMAAFAAWEASKEK
                     KDVVDTGLVLARRFGYPVVVAAVDGSPAAAAGVKSDDVVEKVDGQLTRNMPLWELDAK
                     LSGKAGGRVRLEVVREGKPRHRTIDIVRASWAPATPSAQRVDGETVVRIPSFAPGAAA
                     AIRKILEPLDRTHALVLDVRNNATGSFEEAARAAALFVAAGPLGELKGRRIETKTFRA
                     EPGDRVHESRLVVLVDSGTAGPAELFASALREATVRAAAPAPKLVREEKAKKEDEETA
                     DADPGVDTVKGAENLPVRLVGETTVGMGFTAQVVKLSSGGSLKLSVGKIHTLAGKALC
                     PKGLQPDDRVFHGPPDESTGKGDLILKRGLKILGESGRLKAAA"
BASE COUNT          371 a          917 c          943 g          316 t
ORIGIN      
        1 gccgcggaga ccgcggccgc gccggagaag cccgcggcgt cgctcggcgt cgaggaacgc
       61 aagcgcgagc tcgcggagct gcggcgccgg ctcgccgagc tgaagaaccg gttcgagtcg
      121 tcgaagaagc gcgaggccga cctcaagggg cagctcgaga cggccgacct cgacctccag
      181 atccagacgg gcgagcggcg cgtcctcgac ctgcgcaagg ccgaggccga gcgcgaggcg
      241 acccgtgcgg cggcggaccg cgacgtggcg cagagggagg ccgaggcacg gcgctccgac
      301 ctcgggacgc gcatggcggc gctctaccgc ctcgggcgcc tcgggtatct gcggccgctt
      361 gcggcggcgg actcggcggg gtcgtttctg agcggactgc gccttctgac gcacctcgcc
      421 cggcgcgacg cctcgctcct cgcgcgccac gaggaagcgg ccgcgacgct cgccgcacgc
      481 gaggaggagc tcgccgtccg gaagcgcgat ctcgcggacc tgctggccga gagccggaag
      541 aaggaagtcg cgttcgcagc ggcccgcgcg cgcaaggccg cgctcctcgc gcaggtgcac
      601 gagacggccc gcgtcgagca gaagcaggtc tcgacgctcg aggacaagtc cgagcggctc
      661 gcggctctcc tcgacctcct cgaggggcgc gggcgcgcgc tcgcgccggg cgcggcgtcg
      721 atccgcaagt accgcggggc gctggactgg ccgctcaagg gaacggtcgt cgtgccgttc
      781 ggccgcatcg cgaacccgaa gttccccaag acgttcctgc gttcgtcggg gtggacgatc
      841 ggggcgccga cgggcacgcc ggtccgcacc acgttctcgg gtgaggtcgt gtacgcccag
      901 tggctcaagg gctacgggaa cctcgtcgtc gtcgaccacg gggacggcgt cttcacgctt
      961 tacgggcgcc tcgcgacggg gacgatcggg cgcggggagc gggtctcgat cggcgaccgc
     1021 gtcggcatcc tcggcgagtc gccggaggac gagcccgccg gcctctactt cgagatccgc
     1081 gacaaccgca cctcggccga ccccgcaacc tggctccgct gagacgcgta tcatccggcc
     1141 gtgagcaaat cccgcgtcgc cggcctcgtc gcctccgcgt ccctcctcct cgcgctcttc
     1201 gtggtcctcc ccgggatggg cgcggacgag aaggcccagg agaagaacga taccctctac
     1261 cgcccgctcg gcctcttcac ggaggttctc gggctcgtga ggtccaacta cgtcgagccg
     1321 gtcgagctca agccgctcct ctcgggcgcg ttctcgggca tgaccgaggc cctcgacccg
     1381 ttctccgagt acgtgccgcc ggacaagatg gcggcgttcg cggcctggga ggcctcgaaa
     1441 gagaagaagg acgtcgtcga caccggcctc gtcctcgccc ggcgcttcgg ctacccggtc
     1501 gtcgtggcgg cggtcgacgg gagccccgcc gccgcggccg gcgtgaagag cgacgacgtc
     1561 gtcgagaaag tggacggcca gctcacccgg aacatgccgc tctgggagct ggacgcgaag
     1621 ctctccggca aggccggggg ccgcgtgcgg ctcgaggtcg tgcgcgaagg caagccgcgc
     1681 caccggacga tcgacatcgt gcgcgcgagc tgggcgccgg cgacgccgtc ggcgcagcgc
     1741 gtggacgggg agaccgtcgt gaggattccg tccttcgccc cgggcgccgc ggcggcgatc
     1801 cggaagatcc tcgagccgct cgaccgcacc cacgcgctcg tcctcgacgt gcgcaacaac
     1861 gcgaccggca gcttcgagga ggccgcgcgc gccgccgcgc tgttcgtggc ggcgggcccg
     1921 ctcggagagc tcaagggccg ccggatcgag acgaagacgt tccgcgccga gcccggcgac
     1981 cgcgtccacg agagccgcct cgtcgtcctc gtggacagcg gcacggccgg tccggccgag
     2041 ctcttcgcgt cggcgctgcg cgaggccacg gtccgggcgg ccgcgcccgc gccgaagctc
     2101 gtccgcgagg agaaggcgaa gaaggaagac gaagagaccg ccgacgccga ccccggcgtc
     2161 gacaccgtca agggcgccga gaacctgccc gtccggctcg tgggcgagac gaccgtcggc
     2221 atgggtttca cggcgcaggt cgtgaagctc tcgagcggcg gatcgctgaa gctctcggtc
     2281 gggaagatcc acacgctggc cggcaaggcc ctctgtccca agggcctcca gcccgacgac
     2341 cgcgtcttcc acgggccgcc ggacgagagc accggcaagg gcgacctgat cctcaagcgc
     2401 gggctcaaga tcctcgggga gtccggccgg ctcaaagcgg cggcgtgacg tcccggtccg
     2461 gcaagggtag gaactccggc ggaggcttcc gcacggcggt gctgctcgtc ttcgccgtcg
     2521 cgtgcggagc ggcggtcacg tgggccc
//
LOCUS       DAOMFY010000407         2542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_18959_length_2542_cov_6.583836, whole genome shotgun sequence.
ACCESSION   DAOMFY010000407 DAOMFY010000000
VERSION     DAOMFY010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18959_length_2542_cov_6.583836"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>213
                     /locus_tag="PLB01_19300"
     CDS             <1..>213
                     /locus_tag="PLB01_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79501.1"
                     /translation="LAQASFQLLASRVAERRAGDGPEALAVAGLAETPAPAADAAILS
                     RFAEWSLPVLDPLPEGTLRLGIRVVPP"
     assembly_gap    214..246
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <247..2425
                     /locus_tag="PLB01_19305"
     CDS             <247..2425
                     /locus_tag="PLB01_19305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQQ79502.1"
                     /translation="SFALAFALESTEKDEIALTAEEIWESPETAVRRRGRLFSNLQDT
                     LLARLGAAASLSRAVQRSLEERHPTGVDLSPEETWTFLSEEAPLLGEAGVAVRLPGGG
                     TLRRVSLRLVAKEGGGATSDPGVTRFGLDTLVDFDWKVAVGDALLSSAEFEDLAARQM
                     PLVEVRGEWVLLDREHLEQARRILDRRPGGRTTLGELLRLAGGLDPEGAGASVDGVAG
                     EGWLAGLLDPDVARKEIGAFTAPRALTGVLRPYQMRGVAWLRFLLTRGLGACLADDMG
                     LGKTIQFLATLLAAQEAGERIGPSLLVCPTSVAENWVSEAARFAPSLRVAVHHGPGRA
                     SGEEFRRLLSNVDLLVTTYALAHRDRALLSEVNWEYLTLDEAQNVKNPAAAQSRAVRG
                     LKAARRSALTGTPVENRLAELKSIFDFLNPGLLGSDDSFRRHFVVPIERRREPEAAAR
                     LRNVTAPFLLRRAKTDPGVVPDLPEKIETKEYTGLTREQAALYRATTRSLLAGIGRAN
                     RRTRKAKILLLLLRLKQICDHPALFLGDGRLDGRSAKIARLLEMCEETLAEKSPSLLF
                     TQFAEMGHLLVAALKERFGCEVLFLHGGVPRRARAEMVRRFQEDDDPPPFFVLSLRAG
                     GTGLNLTRASHVFHVDRWWNPAVEEQATDRAFRIGQTQHVQVHKFVCRGTLEERIDRM
                     IEDKKHLARSIVGAGEQWIAGLSDDQLAALVALDADTAVSEGH"
BASE COUNT          322 a          976 c          865 g          346 t
ORIGIN      
        1 gctcgcgcag gcctcgttcc agctgctcgc gtcccgcgta gcggagcgcc gcgcgggcga
       61 cggccccgaa gcgctcgccg tcgccggcct cgccgagact cctgcgccgg cggccgacgc
      121 cgcgatcctc tcgcgcttcg cggaatggag cctcccggtc ctcgacccgc tccccgaggg
      181 cacgctgcgc ctcggcatcc gggtcgtgcc gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnctcc ttcgcgctcg ccttcgccct cgagtccacg gagaaggacg agatcgcgct
      301 cacggccgag gagatctggg agtcgcccga gaccgccgtc aggcgccggg ggcggctctt
      361 ctcgaacctg caggacacgc tcctcgcgcg cctcggcgcg gccgcgtcgc tctcccgcgc
      421 cgtccagcgc agcctcgagg agcgccatcc gaccggcgtc gacctctccc cggaggagac
      481 ctggaccttc ctctcggagg aggctcctct cctcggcgag gccggcgtcg ccgtccgcct
      541 gccgggcggc ggcacgctcc ggcgcgtctc gctccgcctc gtcgcgaagg aaggcggagg
      601 ggcgacgtcc gaccccggcg tcacgcgctt cggcctcgac acgctcgtcg acttcgactg
      661 gaaggtcgcc gtcggcgacg cgctcctctc gtcggccgaa ttcgaggacc tcgcggcgcg
      721 gcagatgccg ctcgtcgaag tgcgcggcga gtgggtgctc ctcgaccgcg agcacctcga
      781 gcaggcccgc cggatcctcg accggcgccc cggcggccgg acgacgctcg gcgagctcct
      841 gcgcctcgcg ggaggcctcg atcccgaggg cgcgggcgcc tcggtggacg gcgtcgcggg
      901 agagggctgg ctcgcggggc tcctcgaccc cgacgtcgcg cggaaggaga tcggcgcctt
      961 caccgcgccg cgcgcgctca cgggcgtgct ccggccgtac cagatgcgcg gcgtcgcctg
     1021 gctgcgcttc ctcctgacac gcgggctcgg agcctgcctc gccgacgaca tgggtctcgg
     1081 caagacgatc cagttcctcg cgactctcct cgcggcccag gaggccggag agaggatcgg
     1141 tccgtcgctc ctcgtctgcc cgacgtccgt cgccgagaac tgggtgtcgg aggccgcacg
     1201 gttcgcgccg tcgctccgcg tcgccgtcca ccacggcccc gggcgcgcga gcggcgagga
     1261 gttccggcgc ctcctctcga acgtggacct cctcgtcacg acgtacgcgc tcgcccaccg
     1321 cgaccgcgcg ctgctctccg aggtgaactg ggagtacctc acgctcgacg aggcgcagaa
     1381 cgtcaagaat cccgcagcgg cgcagtcgcg ggccgtgcgc gggctcaagg ccgcgcggcg
     1441 gtctgcgctc acgggcacgc ccgtcgagaa ccgcctcgcg gagctgaagt ccatcttcga
     1501 cttcctgaac ccgggcctcc tcgggagcga cgactccttc cgccgccact tcgtggtccc
     1561 gatcgagcgg agacgcgagc ctgaagcggc ggcccggctg cgcaacgtca ccgcgccgtt
     1621 cctcctgcgc cgcgcaaaga cggacccggg cgtcgtgccg gacctgcccg agaagatcga
     1681 gacgaaggag tacacgggcc tcacgcgcga gcaggcggcc ctctatcgcg cgacgacccg
     1741 ctcgctcctc gcgggcatcg gccgcgcgaa ccggcgcacg cgcaaggcga agatcctcct
     1801 tctcctcctg cgcctcaagc agatctgcga ccacccggca ctcttcctcg gcgacggccg
     1861 cctggacggg aggagcgcga agatcgcgcg cctcctcgag atgtgcgagg agacgctcgc
     1921 cgagaagagc ccgtcgctcc tcttcacaca gttcgccgag atgggccacc tcctcgtcgc
     1981 cgccctcaag gagcgcttcg gctgcgaggt cctgttcctg cacggcggcg tgccgcgccg
     2041 cgcgcgcgcc gagatggtcc gccgcttcca ggaggacgac gaccccccgc cgttcttcgt
     2101 gctgtcgctc cgcgcgggcg ggacgggcct gaacctcacg cgcgcctctc acgtcttcca
     2161 cgtcgaccgc tggtggaacc ccgcggtcga ggagcaggcg acggaccgcg cgttccggat
     2221 cggccagacg cagcacgtac aggtccacaa gttcgtctgc cgcggcacgc tcgaggagag
     2281 gatcgaccgg atgatcgagg acaagaaaca cctcgcgcgc tcgatcgttg gcgccggcga
     2341 gcagtggatc gcaggcctct cggacgatca gctcgccgcg ctcgtcgcgc tcgacgccga
     2401 caccgcggtc tcggagggac actgatgccc cgccgccgca aggtacgggt tccgcgcgcc
     2461 gccgcgcccg acgcgggctg ggcgttccgc ttcctcgcgc ggctcgcgtc gttcggctgg
     2521 gacgaggcgc tcgtggacgg cc
//
LOCUS       DAOMFY010000408         2521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_19248_length_2521_cov_6.742498, whole genome shotgun sequence.
ACCESSION   DAOMFY010000408 DAOMFY010000000
VERSION     DAOMFY010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19248_length_2521_cov_6.742498"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1823)
                     /locus_tag="PLB01_19310"
     CDS             complement(<1..1823)
                     /locus_tag="PLB01_19310"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQQ79503.1"
                     /translation="MLPNALRPGRLVLALSFSLALPAAAGEIRGRLLVSDRGDRPAAG
                     VTVAALPWETPGEEARREAKGGEAPKPFATATTGADGAFVLQVPAEPGKEKLFRVRAE
                     GAGVVPVLFEDVWDVSETDDLGEHLLSAAAALTGTVVDASGAPLAGAEVTLEPGGAGS
                     ADPSFRTLARKVATGPEGVFRFEEASATGNRLSVRKDGLAPSATSGVRSGGLARPLAL
                     SPGVALAGTVRDAQKRPVANALVRFEGVKATTPWVLTGEDGAFTIAHAPDGRGTLVVD
                     AGDSGWASKPDVKLPLAEGRTFPVALAAPASLAGKIVDEKTGRAVPRARLLLKGNAFA
                     RLVRTAPDGSYALKGVPPETYRLSVDEARYVPWSRPSFTLAPAESKRLDIALTLGAAL
                     SGKVVDEAGVPVGAARGTLTRGGENAIAGIRRMLRAGAEVTAFRSRPDGTFTAMRLPP
                     GENQRLFVSHAEFERATVAGLALPAGGTKAGVTVVLRRGATIAGVVKDANEQPVPDAE
                     VQIDPSMNLRGGAGGNVMNLVRLGGQSQRPRVKTGADGTFAIKGMSVGEYAVVVKKPG
                     FATERVDPVKVGEQGVEPVVVTLGPGAAITGAIRKKCGEGA"
     gene            1990..>2521
                     /locus_tag="PLB01_19315"
     CDS             1990..>2521
                     /locus_tag="PLB01_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79504.1"
                     /translation="MKTAATRVYAPPGDFVRLTSDEPLVFELADRLWDPAPADEQEAI
                     RIEILHARHSPRMGGEEGDFLECAGKGGAEASRSPRGDATVFAFEETQRWMHEEHEFR
                     CEIPGALGVRIDFATAHVAARLAPGLHLLLSEKFPLRSSSLMSFLARTLLEAPAAVLL
                     GRRGWQVLHRGRPSSWA"
BASE COUNT          333 a          942 c          888 g          358 t
ORIGIN      
        1 tcggcgccct cgccgcactt cttccggatc gcgcccgtga tcgcggcgcc cggcccgagc
       61 gtgacgacga cgggctcgac gccctgctct ccgaccttca cgggatcgac gcgctcggtc
      121 gcgaagccgg gcttcttgac gacgacggcg tactcgccga cggacatgcc cttgatcgcg
      181 aacgtcccgt cggcgccggt cttcaccctc gggcgctggc tctgcccgcc gaggcggacg
      241 aggttcatca cgttcccgcc cgccccgccg cggaggttca tcgagggatc gatctggact
      301 tccgcgtccg ggacgggctg ctcgttcgcg tccttcacga cgcccgcgat cgtggcgccg
      361 cgccggagca cgaccgtgac gccggctttc gtcccgcccg caggaagcgc gaggcccgcg
      421 accgtcgcac gctcgaactc cgcgtggctg acgaagaggc gctggttttc gccgggcgga
      481 aggcgcatcg ccgtgaacgt gccgtccgga cgcgagcgga acgccgtgac ttccgcgccc
      541 gcgcggagca tccgccggat gcccgcgatc gcgttctcgc ctccgcgcgt cagcgttccg
      601 cgcgccgcgc cgacggggac gcccgcctcg tccacgacct ttccggagag cgccgcgccg
      661 agcgtgagcg cgatgtcgag gcgcttcgat tccgccggcg cgagcgtgaa ggacggccgc
      721 gaccagggca cgtagcgcgc ctcgtcgacg ctcagccggt acgtctcggg cggcacgccc
      781 ttgagcgcat acgacccgtc cggcgccgtc ctcacgaggc gtgcgaacgc gtttcccttg
      841 aggaggaggc gggcgcgcgg caccgcgcgg cccgtcttct cgtcgacgat cttccccgcg
      901 agcgacgcgg gtgcggcgag ggcgaccgga aacgtccggc cctcggcgag cgggagcttc
      961 acgtcgggct tcgacgccca gcccgagtcc ccggcgtcca cgacgagcgt cccgcgtccg
     1021 tccggcgcgt gcgcgatcgt gaaggcgccg tcctcgcccg tcaggaccca gggcgtcgtc
     1081 gccttcacgc cctcgaaacg gacgagggcg ttcgcgaccg gccttttctg cgcgtcgcgc
     1141 acggtgcccg cgagcgcgac gcccggggag agcgcgagcg gccgggcgag accgcccgat
     1201 ctcacgccgg acgtggcgga cggcgcgagc ccgtccttcc gcacgctcag gcggtttccc
     1261 gtcgcgctcg cctcctcgaa ccggaagacg ccctccgggc ccgtcgcgac ttttcgcgcg
     1321 agtgtccgga acgacgggtc cgccgagccc gcgccgcccg gctcgagcgt cacctcggcg
     1381 cccgcgagcg gcgcgccgga agcgtcgacg acggtccccg tgagcgccgc agccgcggag
     1441 aggaggtgct cgccgaggtc gtccgtctcg gagacgtccc agacgtcctc gaagagaacg
     1501 ggaacgacgc ccgcgccctc cgcacgtacg cggaagagct tctccttgcc cggctcggcc
     1561 gggacctgga gcacgaacgc gccgtcggcg cccgtcgtcg cggtcgcaaa cggcttcggc
     1621 gcctcgccgc ccttcgcctc gcggcgcgcc tcctcgcccg gcgtctccca cgggagggcc
     1681 gcaaccgtca ctcccgcggc ggggcggtcg ccccggtccg acacgaggag ccggccgcgg
     1741 atctcgccgg cggccgcggg gagcgcgaga gagaaggaga gggcgaggac gaggcggccg
     1801 ggccgaagcg cgttcgggag cattgtcacg gaggaaacct ccacgtcgaa ttgaccctac
     1861 aggggccgga cgctcgctct cgagcaacct cgctgccacc ccgggcgcgt gccgggggcg
     1921 ccctgcgcgc gcatcccggt cagacgcgcg ggaacgtcga aaggttacgc accgtggcaa
     1981 gctgctcgcg tgaaaaccgc ggcgacacgg gtttacgcgc cccccggaga cttcgttcgg
     2041 ctgacgtcgg acgagccgct cgtcttcgag ctcgcggacc ggctctggga tcccgcgccc
     2101 gcggacgaac aggaggcaat tcggatcgag attctgcatg cgcggcattc gccgcgcatg
     2161 gggggagaag agggggattt cttagaatgc gcgggcaagg gaggcgcgga ggcgagtcgc
     2221 tcgccgcggg gcgacgccac cgtttttgcc ttcgaagaaa cgcagcggtg gatgcacgaa
     2281 gagcatgaat tccgctgcga gattccgggc gcgctgggcg ttcgaatcga cttcgcgacg
     2341 gcccacgtcg ccgcgcgcct cgctcccggc ctgcatctct tactttcaga gaaatttcct
     2401 cttcgatcct cttctctcat gtcctttctc gcccgcacgc tcctcgaggc gccggcggcc
     2461 gtcctcctcg gccgccgcgg ctggcaggtc ctgcatcgag gtcgtccgtc gtcatgggcg
     2521 c
//
LOCUS       DAOMFY010000409         2506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_19454_length_2506_cov_7.602203, whole genome shotgun sequence.
ACCESSION   DAOMFY010000409 DAOMFY010000000
VERSION     DAOMFY010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19454_length_2506_cov_7.602203"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLB01_19320"
     CDS             <1..658
                     /locus_tag="PLB01_19320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HQQ79505.1"
                     /translation="AQGILAIEHPTVGAGEQGLIVADALLAARAAGAANELVAFVDDE
                     PSRAGTNVLEIPVLGPLAVLGGAAHDAVIVAIGDNRRRREVSAALAAAGERFAIARHP
                     FSSVARDVMLPPGAMVSAGVVVSPRATLGEGVLLNTNACVDHDTAVGAYAHVSVGATV
                     GARCVIGGETLIGIGACVLSGVRVGAGTVVGAGAVVVRDLPDGVVAYGNPARVRRAHA
                     "
     gene            complement(666..1571)
                     /locus_tag="PLB01_19325"
     CDS             complement(666..1571)
                     /locus_tag="PLB01_19325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ79506.1"
                     /translation="MSLAVSRLGAADVDECAWLDALNVCFPGWGGADRFAWCFRRAGA
                     GRTPDALALTDRGRVVAGSGVTYRTLVLTDGRRVPAAIMTGSWTLPEARGRGAFTRLV
                     AESRAAAAARGAPILLAFVTEGNASRRRLEAAGAEMLPSFYCRAAGAASGAGQLENLG
                     EPDPATFRTAPGFARFTYEDEEWRGQFVERPGGVRVLGVRGSFAALVEEGPDRRVHAL
                     AAAPPFVGAAHAALAADAARAGRATFAFTTRPALAAELERLGFTIGRGFVTALPSAAP
                     SLTAALETDDARRLLNLDLVSGDRM"
     gene            complement(1568..>2506)
                     /locus_tag="PLB01_19330"
     CDS             complement(1568..>2506)
                     /locus_tag="PLB01_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79507.1"
                     /translation="VPAWLPPGARAAVCFSVDDVHPAGTEPGGEPDLARRALDVVAWL
                     QDRHPRLAVTFFTTPDWRSASPAPEPGVVGRIPILGKFVYQARVLPRNTCRLSRHPAF
                     CSTLRSWPRSEIALHGLHHVRRGPSTVAEFEGLGPAACRALLARAQVLFDEAGLPCVR
                     GLCPPGWLATPALLRAMDEIGMAFLASARDLESPVSPEGVTRGSGLTGVPLFRPARLP
                     GTRLVHLSTNFQATSSLDRARAIVETGGLLSIKAHLLQGLGRYRALDGLGKDYAEHLH
                     RVLSTLEDEHGDALWWPRMSAIADRVRADANAEACA"
BASE COUNT          362 a          885 c          962 g          297 t
ORIGIN      
        1 ggcccagggc atcctcgcca tcgaacatcc aaccgtcggc gccggagagc aggggctgat
       61 cgtcgccgac gcgcttctcg cggcccgcgc cgcaggcgcc gcgaacgaac tcgtggcatt
      121 cgtcgacgac gagccgtccc gggcggggac gaacgtcctg gagattccgg tactcggccc
      181 cctcgcggtt ctcggcggag cggcgcacga cgccgtgatc gtcgcgatcg gggacaaccg
      241 gaggcggcgc gaggtgtcgg ccgcactcgc ggcagcgggc gagcgcttcg cgatcgcgcg
      301 ccatccgttc tcgtccgtcg cgcgggacgt catgcttccg cccggcgcca tggtttcggc
      361 gggcgtcgtc gtgtcgcccc gcgcgacgct gggcgaaggc gtgctcctga acaccaacgc
      421 gtgcgtcgac cacgacacgg ccgtcggcgc atacgcccac gtgtctgtcg gagcgaccgt
      481 cggggcccgc tgcgtgatcg gcggcgagac gctcatcggc atcggcgcct gcgtgctctc
      541 gggcgttcgg gtcggggccg ggacggtcgt tggcgcgggt gcggtcgtcg tgcgggacct
      601 gccggacggc gtcgtggcgt acggaaaccc ggcgcgcgtc cggcgcgcgc acgcctgacg
      661 gcgctctaca tccggtcgcc gctcacgagg tcgagattga ggaggcggcg ggcgtcgtcg
      721 gtttcgagag cggcggtgag ggaaggcgcg gcggagggca gtgccgtcac gaagccacgc
      781 ccgatcgtga agccgagacg ctcgagttcc gccgccaatg cgggtcgggt cgtgaacgcg
      841 aacgtggcgc ggccggctcg cgcagcatcg gcggcgagag ccgcgtgcgc ggcgccgacg
      901 aagggcgggg ccgcggcaag cgcatgaacg cggcgatccg ggccttcctc gacgagcgcg
      961 gcgaacgacc cgcggacccc gaggacgcgg acgccgccgg gccgctccac gaactgcccg
     1021 cgccattcct cgtcctcgta ggtgaaacgg gcgaaacccg gtgccgtgcg gaacgtggcg
     1081 ggatccggct cgccgagatt ctcgagctgt cccgctcctg acgccgcgcc cgcggcacgg
     1141 cagtagaagc tgggaagcat ctcggcgccg gcggcctcga ggcgccgccg gctcgcgttt
     1201 ccttccgtca cgaacgcgag aaggatcggg gcgccccgcg cggccgccgc tgcgcgcgac
     1261 tccgcgacga gccgcgtgaa ggcgccgcgc ccgcgggctt ccggaagcgt ccacgagccg
     1321 gtcatgatcg cagccggtac gcgccggccg tcggtgagca ccagagtccg gtacgtcacg
     1381 cccgaccccg cgacgacgcg gccccggtcg gtcagggcga gcgcgtccgg cgtccggccg
     1441 gctcccgcgc ggcggaagca ccacgcgaag cgatcggcgc cgccccagcc ggggaagcag
     1501 acgttcaggg cgtcgagcca cgcgcactcg tcgacgtccg ctgcgcccag acgcgagacg
     1561 gcaaggctca cgcgcacgcc tccgcgttcg cgtcggcgcg tacacgatct gcgatcgcag
     1621 acatccgcgg ccaccagagc gcgtcgccat gctcgtcctc cagcgtcgag aggacgcgat
     1681 ggaggtgctc cgcgtagtct tttccgaggc cgtcgagcgc gcggtagcgg ccgaggccct
     1741 ggagcaggtg agccttgatc gagaggaggc cccccgtctc gacgatcgcg cgcgcccggt
     1801 cgaggctcga cgtggcctgg aaattcgtgg acaggtgcac gaggcgcgtg ccgggaagcc
     1861 gcgcgggccg aaaaaggggc acgccggtga gcccgctccc gcgcgtgacg ccctccggcg
     1921 ataccggcga ctcgaggtcg cgagcggaag cgagaaacgc catgccgatt tcgtccatgg
     1981 cgcgcaggag ggcgggagtc gcgagccagc ccggcggaca gagcccgcga acgcacggca
     2041 ggccggcctc gtcgaacagg acctgggcgc gcgcgagaag cgcgcggcag gcggcagggc
     2101 cgagtccctc gaactcggcc acggtgctgg gcccgcggcg gacgtgatgg aggccgtgga
     2161 gcgcgatctc cgagcggggc caggaccgca gcgttgaaca gaacgccggg tggcggctga
     2221 gccggcaggt gtttcttgga agcacccgcg cctggtacac gaacttcccg aggatcggga
     2281 tcctccccac gacgccgggc tcgggcgccg gcgaagcgga tcgccagtcc ggcgtcgtga
     2341 agaacgtgac cgcgagccgc ggatgccgat cctggagcca tgcgacgacg tcgagggcgc
     2401 gccgcgcgag gtccggttcg ccgcccggct ccgtcccggc gggatggacg tcgtcgacgg
     2461 aaaagcacac ggccgcgcgc gcgcccggag ggagccacgc gggaac
//
LOCUS       DAOMFY010000410         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_19560_length_2499_cov_7.197218, whole genome shotgun sequence.
ACCESSION   DAOMFY010000410 DAOMFY010000000
VERSION     DAOMFY010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19560_length_2499_cov_7.197218"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLB01_19335"
     CDS             complement(<1..430)
                     /locus_tag="PLB01_19335"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ79508.1"
                     /translation="MLSFEVRKTGGESEIRKVHRDVIHIGASTGNDLVVRARGVLGRH
                     ARISVAGEELRLEVLGSAPGSDVTLNGSVVKTAALAAGDRINIGEATITLLNGPAPNP
                     NRISAPPPRGRDAFSLASAALSPLPAPPAGPPPAPAPLPRH"
     gene            583..1587
                     /locus_tag="PLB01_19340"
     CDS             583..1587
                     /locus_tag="PLB01_19340"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQQ79509.1"
                     /translation="MPASPLDGPPFRGRNAQTLKRIVDTVRSVLEPDVVDLEYVFEPQ
                     GQRYRWRMTGSQVTPLKAVRTAIRGGGPSVLSAPFAVPGLVRGVLALARGPRRPWTGP
                     ESLRFEVLRPLLAQLLEFAAACEIHAAARRRLESAADATPTPVFLVRASGTILYANAA
                     AAQLLSSRGAEPHVAAEGARDVPLHGWLQRLALSAPAGTKRRGSLSDGRHFEASVARF
                     SDEEEDVAVLTLRLLSTVSPEDVRTRLAERGVSGREAEVVGAVLEGLRNAEIARRLFI
                     TEWTVKDHLKHVFSKLGVSSRGGLLRALDAGPGFPCPEETRPARPQPVESRTRRTLKM
                     "
     gene            1669..>2499
                     /locus_tag="PLB01_19345"
     CDS             1669..>2499
                     /locus_tag="PLB01_19345"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQQ79510.1"
                     /translation="MFRLKKLDIQGFKSFVDKTSVTLPTRITAIVGPNGSGKSNICDA
                     VQWVLGEQSARNLRGTTMEDVIFNGAARRRPLGMAEVTITLESRSGEEWAETGGEVTI
                     ARRVTRDGESDYILNGKRSRLKDVQEILLGTGLGVRAYSIIEQQKIDLILSTKPQDRR
                     RLIEEAAGVSKYKIRKRQAEVKLEETRGNLLRLTDIVSEIDRTCASLKRQASRAARWK
                     EQADVLAREKRRLARLRADRLTSGFAEGEKALSAATDAEAAALAELGRLEAHLAEARR
                     L"
BASE COUNT          374 a          905 c          885 g          335 t
ORIGIN      
        1 cgtggcgcgg cagcggcgcg ggagcgggcg ggggaccggc gggaggcgcc ggcaagggag
       61 agagagccgc cgacgcgagg gaaaaggcgt cgcggccgcg cggcggaggc gccgagatgc
      121 ggttcgggtt gggcgcgggg ccgttcagga gcgtgatcgt ggcctctccg atgttgatcc
      181 ggtcgccggc ggcgagcgcc gccgtcttga cgaccgagcc gttcagcgtg acgtcggacc
      241 ccggcgcgct cccgaggacc tcgaggcgga gctcctcgcc cgccaccgag atgcgggcgt
      301 gccgcccgag cacaccccgg gcgcgcacga cgaggtcgtt acccgtcgag gcgccgatgt
      361 ggatgacgtc gcgatggacc ttgcggattt cggattcgcc gcccgtcttg cggacctcga
      421 agctcaacat gagaagtgtc ctccgccaga ggaagggggg gtgctttgat caaacaacag
      481 actatcacgc ggaagggcgt tgtccagtgg gcgtcgacac gaattgaacg ggcccgttcg
      541 aggcaggccg tagaatgcgg gcgaaggctg accgcacccc acatgcccgc ttcccccctc
      601 gacgggccgc ccttccgcgg ccgcaacgcc cagacgctga agcggatcgt cgatacggtc
      661 cggtcggtcc tcgagcccga cgtcgtcgac ctcgagtacg tcttcgagcc ccaggggcag
      721 cgctaccgct ggcggatgac gggcagccag gtcacgccgc tgaaggcggt gaggaccgcc
      781 atccgcgggg gcggcccctc cgtcctgtcc gccccgttcg cggtgccggg gctcgtccgc
      841 ggcgttctcg ctctcgcgcg cgggccgcgc cgcccctgga cgggacccga gtcgctccgc
      901 ttcgaggtcc tccgccccct gctcgcccag ctcctcgagt tcgccgccgc gtgcgagatc
      961 cacgccgcgg cccgccggcg cctcgagagc gcggccgacg cgacgccgac acccgtcttc
     1021 ctcgtgcgcg cgtctggcac gatcctgtac gcgaatgcgg ccgcggccca gcttctttcc
     1081 agccgcggag ccgagccgca cgtcgcggcc gagggcgccc gggacgtgcc gctgcacggg
     1141 tggctccagc gcctcgcact ctccgctccc gcggggacga agcgccgcgg ctcgctctcg
     1201 gacggccgcc acttcgaggc ctccgtcgcg cgtttctccg acgaggagga ggacgtcgcc
     1261 gtcctcacgc tgcgcctcct ttcgacggtc tcgcccgagg acgttcgcac gcgcctcgca
     1321 gagcgcggcg tctccggccg cgaggcggag gtcgtcggcg cggtgctcga gggcctgcgc
     1381 aacgcggaga tcgcgcgccg cctcttcatc acggaatgga cggtcaagga tcacctgaag
     1441 cacgtcttct ccaagctcgg cgtgtcctcg cgcggcggcc tgctgcgggc gctggacgcc
     1501 ggccccggct tcccctgccc ggaggagacg cggccggcca gaccccagcc ggtggagtcc
     1561 cgcactcgcc gcaccctcaa aatgtagtgg ttgggtccgg ggccgcgtgg tagcgtcccg
     1621 ccccgaagaa gcgtcggcca cgtccggaac tgcggaaaaa aggcgttcat gtttcgactg
     1681 aagaaactcg acatccaggg cttcaagtcc ttcgtggaca agacctcggt cacgctcccg
     1741 acgcgcatca cggccatcgt cggtcccaac ggctcgggca agtcgaacat ctgcgacgcc
     1801 gtgcagtggg tcctcggcga acagtccgcc cgcaacctgc gcggaacgac gatggaagac
     1861 gtcatcttca acggagcggc ccgccgccga ccgctcggaa tggcggaagt cacgatcacg
     1921 ctcgagtcgc gcagcggcga ggagtgggcc gagaccggcg gcgaggtcac gatcgcgcgc
     1981 cgcgtcacgc gcgacggcga gagcgactac atcctgaacg gcaagcgctc gcgcctgaag
     2041 gacgtgcagg aaatcctcct gggcacgggc ctcggcgtcc gcgcgtactc gatcatcgag
     2101 cagcagaaga tcgacctgat cctctcgacg aagccgcagg accggcggcg actcatcgag
     2161 gaggccgcgg gcgtctcgaa gtacaagatc cggaagcgcc aggccgaggt gaagctcgag
     2221 gagacgcgcg ggaacctcct gcgtctcacg gacatcgtct cggagatcga ccgcacgtgc
     2281 gcgtctctca agcgccaggc ctcccgcgcc gcgcgctgga aggagcaggc cgacgtcctc
     2341 gcccgtgaga agaggcggct cgcgcgcctc cgcgcggacc ggctcacgtc cggcttcgcc
     2401 gagggcgaga aggccctctc cgccgcgacg gacgccgagg ccgccgcgct cgccgagctc
     2461 ggccgcctcg aggcgcatct cgcggaggcg cgccgcctc
//
LOCUS       DAOMFY010000411         2472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_19955_length_2472_cov_6.361192, whole genome shotgun sequence.
ACCESSION   DAOMFY010000411 DAOMFY010000000
VERSION     DAOMFY010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19955_length_2472_cov_6.361192"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLB01_19350"
     CDS             <1..342
                     /locus_tag="PLB01_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQQ79511.1"
                     /translation="LEAGLLAAVPGARDNAAGGTRLPTATSVVFPGAEAETLVAALDL
                     EGIAVSAGSACHAGTTAPSRVLIALGLTPADAKATLRISFGRTSTERDLERLLEAVPR
                     VVAQVRRAARA"
     gene            404..1123
                     /locus_tag="PLB01_19355"
     CDS             404..1123
                     /locus_tag="PLB01_19355"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ79512.1"
                     /translation="MIIQCSSCQARYHYDESRFAGSPAKRIKCTKCASVFEIRNPGLA
                     GSVAAGLARPHSIGPDEFSLDETAMTEASKRRQAGAMPGGPARAAILATTPPGETSPP
                     PQADPGRPTTDKVPVPAASRGMARPLRLPDGVRLSLACISGPDSGRIFEIDRPRVVIG
                     RANADIVVSDIQCSRQHAAIEVMDEHVFLVDLGSTNGTYVFDQRIGRVELENRTEFEI
                     GTTTLMLIRSGGPHENQPPGS"
     gene            complement(1133..>2472)
                     /gene="thrC"
                     /locus_tag="PLB01_19360"
     CDS             complement(1133..>2472)
                     /gene="thrC"
                     /locus_tag="PLB01_19360"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQQ79513.1"
                     /translation="PAMPDLISTSAMPAPSVAARFRCVACAAEYPLAEARTACAACGE
                     LLDVAPDLESFGLSGTAWRDLFDRRRAAPGSPGTRAFERSGVWRYREHVLPGLGEEHL
                     VSKPEGSTRLYAGGALGDELGLTRLFVKHEGENPTLSFKDRGMAAGVTWAKACGFPLV
                     GCASTGDTSAALAAYAAEAPGMRAVVLLPHAKITDEQLSQALAYGATTLGLDTDFDGC
                     MRIVAALADEGRVYLLNSKNAVRLEGQKTIGFEAIHDLGWAVPDWFVVPVGNAGNVSA
                     IGKGLREWLSLGILDRKPRIAGIQADAADPFYLSYEGGFKERVTRVAADTAASAIRIG
                     APVSHPKAQREIGFFDGVVERVTEPELLDAFALANRHGLAICPNSAVALAGAAKLRRE
                     GVIRKDDLVVVVATAHALKFSGTMAAYHRGSGRLANPPRRLPATLDAVRAALG"
BASE COUNT          350 a          913 c          880 g          329 t
ORIGIN      
        1 ctcgaggctg gccttctcgc ggccgtcccc ggagcccggg acaacgcggc gggcggcacg
       61 cgcctcccga cggcgacgtc cgtcgtgttc cccggcgccg aggccgagac gctcgtcgcg
      121 gcgctcgacc tcgagggcat cgccgtttct gcgggatcgg cctgccacgc cggaacgacg
      181 gctcccagcc gcgtcctcat agccctcggc ctcacgccgg ccgacgcgaa ggcgacgctc
      241 cgaatctcct tcgggcgtac gtccacggaa cgcgacctgg agcgcctgct cgaggccgtt
      301 ccgcgcgtcg tcgcgcaggt gcggcgcgcg gcgcgcgcct gactgcggtc cgcgcgcccg
      361 gccccggaac cggctaaaat ccaggccgaa ggtccagtcc gtcgtgatca tccagtgctc
      421 ttcgtgccag gcgcgttatc actacgacga gtcgcgcttt gccggctcgc ccgccaagcg
      481 catcaagtgc acgaaatgcg ccagcgtctt cgagatccgg aacccgggcc tcgcgggctc
      541 cgtcgcggcc ggactggcgc ggccccactc gatcgggccc gacgagtttt cactcgacga
      601 gaccgcgatg accgaggcga gcaagcgccg ccaggcagga gcaatgccgg gcgggcctgc
      661 ccgcgcggcc atcctggcga cgacgccgcc cggagaaacg tccccgccgc cgcaggccga
      721 cccggggcgc ccgacgacgg acaaggtgcc cgtccccgcg gcctccaggg gcatggcgcg
      781 cccgctccgg ctgcccgacg gcgtccgcct gtcgctcgcg tgcatctcgg gcccggactc
      841 gggccgcatc ttcgagatcg acaggccgcg cgtcgtgatc gggcgcgcga acgccgacat
      901 cgtcgtgtcc gacatccagt gctcgcgtca gcacgccgcc atcgaggtca tggacgagca
      961 cgtgttcctc gtggacctcg gctcgacgaa cgggacgtac gtcttcgacc agcggatcgg
     1021 ccgcgtcgag ctcgagaacc gtaccgagtt cgagatcgga acgacgacgc tcatgctcat
     1081 ccggagcggc ggaccgcacg agaaccagcc cccgggcagc tgagcagggc ctctagccga
     1141 gcgcggcgcg aaccgcgtcg agcgtcgccg ggaggcggcg cggcgggttc gcgaggcggc
     1201 cgctcccgcg gtggtaggcg gccatcgtcc ccgagaactt gagcgcgtgc gccgtcgcga
     1261 cgacgacgac gaggtcgtcc ttccggatga cgccctctct ccggagcttc gcggcgcccg
     1321 cgagcgcgac ggccgagttc gggcagatcg caaggccgtg ccggttcgcg agcgcgaacg
     1381 cgtcgaggag ctcgggctcg gtcacccgct cgacgacgcc gtcgaagaag ccgatctccc
     1441 gctgcgcctt cgggtgcgag acgggcgcgc cgatccggat cgccgatgcc gccgtgtcgg
     1501 ccgcgacccg cgtgacgcgc tctttgaacc cgccctcgta cgaaaggtag aacgggtccg
     1561 ccgcgtccgc ctggatgccc gcgatgcggg gtttcctgtc gaggatgccg agcgagagcc
     1621 actcgcgaag gcccttgccg atcgccgaga cgttccccgc gttgccgacg ggcacgacga
     1681 accagtccgg aaccgcccac ccgaggtcgt ggatcgcctc gaagccgatc gtcttctgtc
     1741 cctcgaggcg gacggcgttc ttcgagttca ggaggtacac gcgcccctcg tctgcgagcg
     1801 ccgcgacgat cctcatgcag ccgtcgaagt ccgtgtcgag gccgagcgtc gtggccccgt
     1861 acgcgagagc ctgcgagagc tgctcgtccg tgatcttcgc gtgcggcagg agaaccacgg
     1921 cgcgcattcc cggcgcctcg gccgcatagg ccgcgagcgc cgccgaggtg tcgcccgtcg
     1981 acgcgcagcc cacgagcggg aacccgcagg ccttcgccca cgtgacgcct gcggccatcc
     2041 cacggtcctt gaacgacagc gtcgggttct cgccctcgtg cttgacgaag aggcgggtga
     2101 gcccgagctc gtcgccgagc gcgccgcccg cgtagaggcg tgtcgagccc tcgggcttgc
     2161 tcacgaggtg ctcttcgccg aggccgggca ggacgtgctc gcggtagcgc cagacgccgc
     2221 tgcgctcgaa tgcgcgcgtg ccgggcgagc cgggcgcggc gcgccggcgg tcgaagaggt
     2281 cgcgccaggc ggtccccgag aggccgaacg actcgagatc cggcgcgacg tcgaggagct
     2341 ctccgcacgc cgcgcaggcg gtgcgcgcct cggcgagcgg gtattccgcc gcgcaggcga
     2401 cacagcggaa gcgggcagcg acggagggcg ctggcatcgc ggaagtactg atcaggtccg
     2461 gcattgccgg gc
//
LOCUS       DAOMFY010000412         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_20194_length_2454_cov_6.516465, whole genome shotgun sequence.
ACCESSION   DAOMFY010000412 DAOMFY010000000
VERSION     DAOMFY010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20194_length_2454_cov_6.516465"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PLB01_19365"
     CDS             complement(<1..1373)
                     /locus_tag="PLB01_19365"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HQQ79514.1"
                     /translation="MASSPLRVFFLWHLHQPWYVPFDGEPAPLPWVRLHALKDYAALP
                     ALFAASPRVPHAVNLVPGLLDQIEMLTKGGTDAFLEIAKAPAGGWDAGARRFALANFF
                     SVNDRILARFPRYADLKRRADAGDAFSEADFRDLVVLFHLAWSSPELLKDPLLVHLRK
                     RGHAFTEQEKTALLDRQTAALSDVLPAWKAAFAGGNVEAATSPYHHPILPLLADSSVA
                     RQTLPDAPLPSPRFAHPDDARSQIALGLSTFERHFGFRPKGMWPPEGALSETALALLG
                     EAGVSWVATDEALLFQSPPVSRRSFGEGDRARTLFRPWSLPGISSPAVFFRDRVLSDR
                     IGFSYATWAADDAAADFVARLLSIRAAAPDAALTVPVILDGENAWETYPENGEPFLRA
                     LASALEGRDDIRVVTPSQALAEAPPAPLEGLVAGSWVNGTLATWIGSPVKNRAWTLLG
                     AARDEAKS"
     gene            1459..1557
                     /gene="ffs"
                     /locus_tag="PLB01_19370"
     ncRNA           1459..1557
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLB01_19370"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1715..>2454
                     /gene="dnaX"
                     /locus_tag="PLB01_19375"
     CDS             1715..>2454
                     /gene="dnaX"
                     /locus_tag="PLB01_19375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQQ79515.1"
                     /translation="MSDANWVPLARKWRPRTFADVVGQEEIVRALTNAVTADRLAHAY
                     LFSGPRGVGKTTTARLLAAAINCNSSDRPTATPCGTCASCQEVLEGRSIDTLEIDGAT
                     HGKVDQARDLIEVVSYAPSRDRRKVFIIDEVHAISSAAFQALLKTLEEPPAHAVFILA
                     TTERHKIPATILSRCQRFDFRRLTDDEVADRLADVAGREGFAVVESASAAKKGSPFVE
                     RPALGALAAAATGSLRDGLSLFDQAAARS"
BASE COUNT          365 a          851 c          918 g          320 t
ORIGIN      
        1 gacttcgcct cgtcgcgcgc ggctccgagg agcgtccacg cgcggttctt cacgggcgag
       61 ccgatccacg tcgcgagcgt tccgttcacc cacgagcccg cgacgagtcc ctcgagcggc
      121 gcgggcgggg cctcggcgag cgcctgcgaa ggcgtcacga cccggatgtc gtcgcgcccc
      181 tcgagcgcgg aggcgagcgc acggaggaac ggctcgccgt tctcgggata cgtctcccag
      241 gcgttctcgc cgtcgaggat gacgggcacg gtgagcgcgg cgtcgggcgc ggccgcgcgg
      301 atcgagagca ggcgcgcgac gaagtctgcg gcggcgtcgt ccgcggccca cgtcgcgtac
      361 gagaaaccga tgcggtccga gaggacgcgg tcgcggaaga atacggcggg cgacgagatc
      421 cccggaaggc tccacggccg gaagagcgtc cgcgcgcggt cgccctcgcc gaaagaccgg
      481 cgggagacgg gcggcgactg gaagaggagc gcctcgtccg tcgcgaccca ggagacgccg
      541 gcctcgccga gcagcgcgag cgccgtctcc gagagcgcgc cctcgggcgg ccacatgccc
      601 ttcgggcgga agccgaagtg tcgttcgaac gtggaaaggc cgagcgcgat ttgcgagcgc
      661 gcgtcgtcgg ggtgggcgaa acgcggcgac gggaggggcg cgtcgggcag cgtctgccgc
      721 gcgacgctcg agtcggcgag gagcggaagg atcgggtgat ggtacgggct cgtcgcggcc
      781 tcgacgttcc cgcccgcgaa cgccgccttc catgccggga ggacgtccga gagcgcggcc
      841 gtctggcggt cgaggagggc ggtcttctcc tgctcggtga aggcgtgccc gcgcttgcgg
      901 aggtggacga ggagcgggtc cttgaggagc tccgggctgg accacgcgag gtggaagaga
      961 acgacgaggt cgcggaagtc ggcctcggag aacgcgtcgc ccgcgtcggc ccgccgcttc
     1021 aggtcggcgt agcgcggaaa gcgcgccagg atccggtcgt tgacggagaa gaagttcgcg
     1081 agggcgaatc ggcgcgcgcc cgcgtcccac ccgcctgcgg gggccttcgc gatctcgagg
     1141 aacgcgtccg tccccccttt cgtcagcatc tcgatctggt cgaggagccc cggaacgagg
     1201 ttcaccgcgt ggggaacccg ggggctcgcc gcgaagagcg cgggcagcgc cgcgtagtcc
     1261 ttgagcgcgt gaagccggac ccagggcagc ggggccggct cgccgtcgaa cggcacgtac
     1321 cacggctggt ggaggtgcca gaggaagaag acgcggagag gagacgaggc caagacggga
     1381 cggttttaca gaacggaggc ggacggcaca agggacgcca ccgctgttag tatgggcacc
     1441 ccccgggccc ccggatgagt tcgggtcccg cgcgacggtc ccctccgaac cgtgtcaggt
     1501 ccggaaggaa gcagcactca ggagtaaggc ccgggcgccg cggatcgccc ggactcatcg
     1561 gggggctcgg ggagcccccc gagagtccga tcgtttctgt tcctgttccg ggggtgcgac
     1621 gtctgccgac atatcgggcg gcgccgggtt cgcacccccg gaccccctga gtcggggggc
     1681 tcgggggtct ttttttcgag tacagtcacc ctccgtgagc gacgcgaact gggtccccct
     1741 cgcccgtaag tggcgcccgc gcacgttcgc ggacgtggtc ggccaggaag agatcgtccg
     1801 ggccctcacg aacgccgtga cggccgaccg tctcgcccac gcatacctct tctcgggccc
     1861 tcgcggcgtc ggcaagacga cgacggcgcg gcttctcgcg gcggccatca actgcaacag
     1921 ttcggaccgg cccacggcga cgccctgcgg gacgtgcgcc tcgtgccagg aggtcctcga
     1981 ggggcgctcg atcgacaccc tcgagatcga cggcgccacg cacggcaagg tcgaccaggc
     2041 gcgcgacctc atcgaggtcg tctcgtatgc gccgtcgcgc gaccgccgca aggtcttcat
     2101 catcgacgag gtccacgcga tctcctcggc ggcgttccag gccctcctca agacgctcga
     2161 ggagccgccc gcgcacgccg tcttcattct cgcgacgacc gagcgccaca agatcccggc
     2221 cacgatcctc tcgcgctgcc agcgcttcga cttccgccgc ctcacggacg acgaggtcgc
     2281 cgaccggctc gcggacgtcg cggggcgcga gggcttcgcc gtcgtcgagt ccgcctcggc
     2341 cgcgaagaag gggtcgccgt tcgtcgagcg gcccgcgctc ggcgcgctcg ccgccgcggc
     2401 gacgggaagc ctgcgcgacg ggctctcgct cttcgaccag gccgccgcac gctc
//
LOCUS       DAOMFY010000413         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_20215_length_2453_cov_4.912427, whole genome shotgun sequence.
ACCESSION   DAOMFY010000413 DAOMFY010000000
VERSION     DAOMFY010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20215_length_2453_cov_4.912427"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /locus_tag="PLB01_19380"
     CDS             <1..1643
                     /locus_tag="PLB01_19380"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQQ79516.1"
                     /translation="LEGETPIQFARRLAKDDATAQRILALPGALTGRGATLSWGALSL
                     ESRRAAVQALFPSDVRATAGWLHIAVEEESLASLAEWFAGDAARAADLARENVFPGGV
                     VPPGATVRIPAELLVPPFRDAEPIPETEPPNLVFGEDERGRYATYRLRKGEALYSAVV
                     VRFTGRLDAADVNELAAKLAERSGIADVHAIPVGYPVKIPVEFLADEFLPKDDPRSVE
                     RAREKAETAQFSRPGVARGLAGVRVILDAGHGGRDTGTLHGGVWEATYVYDVACRLRK
                     LLAEKTRADVVMTTKDGSIGWTVPNQDRLRPRRGQVLLTQPPYGLADPAVGVNLRWYL
                     ANSLLRRPMADGTKVAPEKTVFLSLHADSLHPSVRGAMVYVPGERYLRDRYGKSGAAY
                     AGYREVKEEPVVSFNKKERVASEGASTALAEGLVAALRDGGLPVHSFSPVRTHVIRSG
                     REWVPAVLRYNRVPNRALVEVANLGNEEDRALLVTKAFRQQLAESLASGLVAFFGGPP
                     PEVYGPLPPPVTMRAAPAPTPRPKPAPTPTPKKKARRR"
     gene            complement(1640..>2453)
                     /locus_tag="PLB01_19385"
     CDS             complement(1640..>2453)
                     /locus_tag="PLB01_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ79517.1"
                     /translation="GWIWVLRFSNGITSAGVAAEDDLARDLRLEEGEPAWQRLLARFP
                     SVRAQFAEARAALPFVHAPRLGFRRARAAGDGWALLPSAAAFADPLLSMGFPLALLGI
                     QRLGLALAGDWGTPRFGKRVRDYGEATLAEADAAARLVGALHAAFDDFPLFAELSKLY
                     FAAASYSESARRLGKVSLASSFLLSKHPAFGPALRRICDAARRPLDPESRPRLLADVA
                     RTIAPFDVAGLGDASRRNWFPVEAGDLRAGAAKLEATGDDVARLLAASGFAA"
BASE COUNT          341 a          899 c          921 g          292 t
ORIGIN      
        1 cgctcgaagg cgagacgccg atccagttcg cgcgccgtct cgcgaaggac gacgcgaccg
       61 ctcagcggat cctcgcgctg ccgggggcgc tcacgggccg cggggcgacg ctctcgtggg
      121 gggcgctctc gctcgagagc cggcgcgccg cagtccaggc cctctttccc tcggacgtcc
      181 gtgcgacggc cggctggctc cacatcgcgg tcgaggagga gagcctcgcg tcgctcgccg
      241 aatggttcgc gggcgacgcg gcgcgggccg ccgacctcgc gagggagaac gtgttcccgg
      301 gcggcgtcgt gccgccgggc gcgaccgtga ggattcccgc ggagctcctc gtcccgccgt
      361 tccgtgacgc cgagccgatc ccggagaccg agccgccgaa cctcgtgttt ggcgaggacg
      421 agaggggccg ttacgcgacg taccggctcc gcaagggcga agccctctat tccgccgtcg
      481 tcgtgcgctt cacgggccgc ctcgacgccg ccgacgtgaa cgaacttgcg gcgaagctcg
      541 ccgagcgctc cgggatcgcc gacgtccacg cgattcccgt cgggtacccc gtgaagatcc
      601 ccgtcgagtt cctcgccgac gagttcctcc cgaaggacga tccccggtcc gtggaacgcg
      661 cgcgcgagaa ggccgagacc gcgcagttct cgcggcccgg ggtcgcgcgc gggctcgcgg
      721 gcgtgcgcgt gatcctcgac gccggccacg gcgggcgcga cacgggcacg ctgcacggcg
      781 gcgtctggga ggcgacgtac gtctacgacg tcgcgtgccg cctccggaag ctcctcgccg
      841 agaagacgcg cgccgacgtc gtcatgacga cgaaggacgg ctcgatcggc tggacggtgc
      901 ccaaccagga ccggctgcgc cctcgccgcg ggcaggtgct cctcacgcag ccgccgtacg
      961 gcctcgcgga tcctgcggtc ggcgtgaacc tgcgctggta cctcgcgaac agcctcctca
     1021 ggcggcccat ggccgacggg acgaaagtcg ccccggaaaa gaccgtcttc ctctcgctcc
     1081 acgccgactc gctccacccg tccgtgcgcg gcgcgatggt ctacgtgccc ggcgagcgct
     1141 acctgcgcga ccgctacggg aagtccgggg ccgcgtacgc cgggtaccgc gaggtgaagg
     1201 aggagcccgt cgtctcgttc aacaagaagg agcgcgtcgc ctccgagggc gcctcgacgg
     1261 cgctcgccga gggcctcgtg gcggcgctcc gtgacggcgg cctcccggtc cacagcttct
     1321 cgccggtgag gacgcacgtg atccggagcg ggcgcgagtg ggtgcccgcc gtcctgcggt
     1381 acaaccgcgt cccgaaccgc gcactcgtcg aggtcgcgaa cctcggcaac gaggaggacc
     1441 gcgcgctcct cgtcacgaag gcgttccgcc agcagctcgc cgagtcgctc gcgtccggcc
     1501 tcgtcgcgtt cttcggcggc ccgccgcccg aggtctacgg gccgctgccg cctcccgtca
     1561 cgatgcgggc ggcgcccgcg ccgacgccga ggcccaagcc cgcgccgacg cccacgccga
     1621 agaagaaggc ccgaaggcgc taggcggcga agccggaggc cgccaggagg cgcgcgacgt
     1681 cgtctcccgt cgcctccagc ttcgcggctc ccgcgcgcag gtcgccggcc tcgacgggga
     1741 accagttgcg gcgcgaggcg tcgccgaggc cggcgacgtc gaagggcgcg atcgtccgcg
     1801 cgacgtccgc gaggaggcgc gggcgggatt ccgggtcgag cggccggcgg gcggcgtcgc
     1861 agatgcggcg gagggccggc ccgaaggcgg ggtgcttcga gagtaggaaa gagctcgcga
     1921 ggctcacctt cccgaggcgc cgcgcggact ccgagtagct cgcggccgcg aaatagagct
     1981 tcgagagctc cgcgaagagc gggaagtcgt cgaaggctgc atggagcgcg ccgacgagcc
     2041 gcgcggccgc gtcggcctcg gcgagcgtgg cctctccgta gtcccggacg cgcttgccga
     2101 aacgcggagt gccccagtcg cctgcgagcg cgaggccgag gcgctggatt cccaggagcg
     2161 cgagcggaaa gcccatcgag aggagcgggt cggcgaacgc cgcggccgaa ggcagcagcg
     2221 cccagccgtc gccggcggcg cgcgcacgcc ggaacccgag acggggcgcg tgaacgaagg
     2281 gcagcgcggc gcgggcttcc gcgaactggg cgcggacgct cgggaagcgc gcgagcaggc
     2341 gctgccaggc cggttcgccc tcctcgaggc gcaggtcgcg cgcgaggtcg tcctcggccg
     2401 cgacgcccgc gctcgtgatg ccgttcgaga accggaggac ccagatccag ccg
//
LOCUS       DAOMFY010000414         2430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_20549_length_2430_cov_6.397053, whole genome shotgun sequence.
ACCESSION   DAOMFY010000414 DAOMFY010000000
VERSION     DAOMFY010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20549_length_2430_cov_6.397053"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1074)
                     /locus_tag="PLB01_19390"
     CDS             complement(130..1074)
                     /locus_tag="PLB01_19390"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQQ79518.1"
                     /translation="MQVLRDPLRSVDLPRGGVVTVGNFDGVHLGHQAMLRDVAARARE
                     AGAPSVVVTFDPHPLKVLHPERAPKMIQTLRQREEAIEACGIDALVLVPFTRDFSLIP
                     AGEFAREVLGKRLQAREVCVGARFVFGRERGGDLELLRRIGTEAGFSVHGLSDVSDAA
                     GPISSTRIRAALAGGDVAGANALLGRPYAMDGIIAKGDRMGRKIGFPTMNLQPGNELY
                     PMDGVYFTSVRIDSFERTFGCVTNIGRRPTVYEDYATTIESYILDFSSDVYGERVRLS
                     FFDRVREEMTFPSMLELTAQIRLDVEATRRYFQAHPVS"
     gene            complement(1087..>2430)
                     /gene="rimO"
                     /locus_tag="PLB01_19395"
     CDS             complement(1087..>2430)
                     /gene="rimO"
                     /locus_tag="PLB01_19395"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQQ79519.1"
                     /translation="SVGIVSLGCPKNLVDTEVMLGHLQREGLTLTDPEKSRVVIVNTC
                     GFIDAAKEESVGAILREVARKEAGEIDRVVVAGCMVQKYGRDLAAEIPEVDHFIGLDE
                     LEKAPAAALGLPSLPRFTDKPLATRLYDDLSPRVLTHRKGYAYLKVSEGCNNPCTFCT
                     IPQMRGLMRSRTIASLVKEAQSLEAQGVKELVLISQDTTRYGEDLGLGRTGLATLVEN
                     LLKETSFPWVRFLYAYPKTLDFGVFELMAREKRFLPYVDMPLQHVSRRILASMKRGGD
                     AASYLRQFEEIRKIVPDISLRSTFIVGFPGEKDEDFLEVKEFLEAVRFENAGVFTYSP
                     EPGSGAEPLGDPIPLEEKSSRRDRLMEVQQKISLSKNRAKRGKTFDALLEGPSDETEM
                     LLEARLAGQAPEIDGRVLINDVPPGWTPRVGEIVRVKITEAHAYDLVGRVVDQVA"
BASE COUNT          394 a          789 c          864 g          383 t
ORIGIN      
        1 cgcacgcggg cccgagcgcc gcgaacgccg cgagcgcgcc gatggcgacc gcctcgacgc
       61 ctcggcccgc ccggcccccg aggaacagga cgagagccgc cgcggcggcg cggagcacga
      121 tctcgacgat cacgagacgg gatgcgcctg gaagtagcgg cgcgtcgcct cgacgtcgag
      181 gcggatctgg gcggtgagct cgagcatgga cgggaaggtc atctcctcgc ggacgcggtc
      241 gaagaacgac agccgtacgc gctcgccgta tacgtccgac gagaagtcga ggatgtagga
      301 ctcgatcgtc gtcgcgtagt cctcgtaaac cgtagggcgg cgtccgatgt tcgtgacgca
      361 cccgaaggtg cgctcgaacg agtcgatccg gacgctcgtg aagtagacgc cgtccatcgg
      421 gtagagctcg ttgccgggct ggaggttcat cgtcgggaag ccgatcttgc ggcccatccg
      481 gtcgcccttc gcgatgatcc cgtccatcgc gtacgggcgc ccgagaaggg cgttcgcgcc
      541 cgcgacgtcg ccccccgcca gcgcggcgcg gatccgcgtg gacgagatcg gcccggcggc
      601 gtccgagacg tccgagaggc cgtggaccga gaagcccgcc tccgtcccga tcctccgcag
      661 gagctcgagg tcgcccccgc gctcgcgccc gaacacgaaa cgcgcgccga cgcacacctc
      721 ccgcgcctgg agccgcttgc cgaggacctc gcgggcgaac tctccggcgg ggatgagcga
      781 gaagtcgcgc gtgaagggca cgaggacgag ggcgtcgatc ccgcaggcct cgatggcctc
      841 ctcgcgctgg cgcagcgtct ggatcatctt cggggcgcgc tcgggatgga ggaccttgag
      901 cgggtgcggg tcgaaggtca cgacgacgga cggcgcgccg gcctcgcgcg cgcgggccgc
      961 cacgtcccgg agcatcgcct ggtgtcccag gtgcacgccg tcgaagttgc cgaccgtgac
     1021 gacgcctccc cggggaaggt cgacggagcg gagcggatcg cggaggacct gcaagcgggg
     1081 cggatcttaa gcgacttggt cgacgacccg tccgacgagg tcgtacgcgt gcgcctcggt
     1141 gatcttcacg cggacgatct cgccgacgcg cggcgtccag ccgggaggga cgtcgttgat
     1201 gagcacgcgg ccgtcgatct cgggcgcctg gccggcgagc cgcgcctcga gcagcatctc
     1261 ggtctcgtcg ctgggccctt cgaggagggc gtcgaacgtc ttgccgcgct tcgcgcggtt
     1321 cttggaaaga gagatcttct gctggacttc catcagacgg tcgcggcgcg aagatttttc
     1381 ttcgagtgga atagggtcgc cgaggggctc ggcgcccgag ccgggctcgg gcgagtacgt
     1441 gaagacgccc gcattctcga agcggacggc ctcgagaaac tctttgactt ctaagaaatc
     1501 ttcgtctttc tctcccggga agccgacgat gaacgtcgag cggagcgaaa tatccgggac
     1561 gatctttcgg atttcttcga attggcggag atagctggcg gcgtcgcctc cacgtttcat
     1621 ggaggcgagg atgcggcgag agacgtgctg cagcggcatg tcgacgtagg gaagaaacct
     1681 cttctctcgt gccatcaatt cgaagactcc gaaatccagc gtcttcggat atgcatagag
     1741 aaaccgcacc catggaaacg atgtttcctt caggagattc tcgacgagcg ttgcgaggcc
     1801 cgtgcggccg aggccgaggt cctcgccgta gcgggtcgtg tcctgggaga tgaggacgag
     1861 ctccttcacg ccctgcgctt cgagggactg cgcctccttc acgagcgacg cgatcgtccg
     1921 cgagcgcatg aggccgcgca tctgcgggat cgtgcagaac gtgcaggggt tgttgcagcc
     1981 ctcggagacc ttcaggtacg cgtagccctt gcggtgcgtg agcacgcgcg gggagaggtc
     2041 gtcgtagagg cgcgtcgcga gcggcttgtc cgtgaagcgc gggagcgacg gcaggccgag
     2101 cgcggccgca ggagccttct cgagctcgtc gaggccgatg aagtggtcca cctcggggat
     2161 ctccgccgcg aggtcccggc cgtacttctg gaccatgcag ccggcgacga ccacgcggtc
     2221 gatctcccct gcctccttgc gcgcgacctc gcggaggatc gcgccgacgg actcctcctt
     2281 ggccgcgtcg atgaagccgc acgtgttcac gatgacgacg cgcgacttct ccgggtccgt
     2341 cagcgtcagc ccctcgcgct ggaggtgccc gagcatgacc tcggtgtcca cgaggttctt
     2401 cgggcagccg agggagacga tgcccacgga
//
LOCUS       DAOMFY010000415         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_20625_length_2425_cov_8.508439, whole genome shotgun sequence.
ACCESSION   DAOMFY010000415 DAOMFY010000000
VERSION     DAOMFY010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20625_length_2425_cov_8.508439"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2392
                     /locus_tag="PLB01_19400"
     CDS             <1..2392
                     /locus_tag="PLB01_19400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQQ79520.1"
                     /translation="GGPGGGPGGMLNAIAADAPTGPDGSFVIDGLKPGQVYDVSALGG
                     GGMGPQKRGIVAPAAGQDIVVSGTGRIAGVVLDARSGQPIRNFSASYEPDRGGGMVLV
                     MRIAGARSGAGVKRDFQTEEGAFALEDVAAGTWTLVVEAKGYQTARVSGLVVEDGGSK
                     DGVEVRATPGAVLKGRVSDAKTGRPVANASVSWEPAGSGGPGGGMFGRGGALMIDGGE
                     DLATDAEGAFTVEGIAPGRVKVTAKSPDYADGSEVAEVKETGGTVEVKLVSGGSVSGV
                     VVANGQPVPGANVSLSAAGEAGFGRILGGGQTTTSDATGRFTFEHLTTGRYTASAGLN
                     GKSSNLADVVLQPGDSRNDVVLTLSAGTSIQGVVSGLPDGWHSQTTVIATGVESFFAT
                     TRVGNDGSFRVDGVPAGPVTLRAQAGDGLGTSRSASKQVTASDDVPVLQTEIVFEQGF
                     TLSGRVVRAGQPVPNAVVAANLQGAGGRQSTTRSDESGAYALLGLQEGAYSVTASGDP
                     LAGGVSMVRQTVNLTGDQSLDLTFPTARVGGVVSDATGKQPLADVSVTLASAAGAATP
                     MQRMTTTDSTGRFQFTDVAPQAYTLNASKPDYQFDKRTVNAADDGSADALAIELQRGQ
                     GIELRVRDGLAGVPLRSVTVRVTDGTGATVSAGSIALDSNGVGDVTSLKPGSYTVYAS
                     ANGYATVTLTGISVPSQTIPVTLTPGGAADITVGPKSFVKGVLRGTLRTAAGTPYPYQ
                     LFATDGRLTINADASGQTGFRRLSNLAPGSYVLALDGGGGTTFTIAEGGITPVQLP"
BASE COUNT          340 a          863 c          923 g          299 t
ORIGIN      
        1 cggcggcccc ggcggcggcc cgggcggaat gctgaacgcg atcgcggccg acgcgccgac
       61 cgggcccgac ggctcgttcg tcatcgacgg attgaagccc ggccaggtct acgacgtatc
      121 ggcgctcggc ggcgggggca tgggtccgca gaaacgcggc atcgtcgcgc cggcggcggg
      181 ccaggacatc gtcgtctcgg gcaccggccg catcgcgggc gtcgtcctcg acgcgaggag
      241 cgggcagccg atccggaact tctcggcgtc gtacgagccc gaccgcgggg gcggcatggt
      301 cttggtcatg cgcatcgcgg gggcgagaag cggggcgggc gtcaagcgcg acttccagac
      361 ggaggaggga gcgttcgcgc tcgaggacgt tgccgcgggc acgtggacgc tcgtcgtcga
      421 ggcgaagggc taccagacgg cgcgcgtctc cggtctcgtc gttgaggacg gcgggtcgaa
      481 ggacggcgtc gaggtgcgcg cgacgccggg cgcggtcctg aagggccgcg tctcggacgc
      541 gaagacggga cggcccgtcg cgaacgcttc cgtctcctgg gagcctgccg gctccggcgg
      601 acccggcggc ggaatgttcg ggcgcggcgg agcgctcatg atcgacgggg gagaggacct
      661 cgcgacggac gccgaaggcg cgttcacggt cgaggggatc gcgcccggcc gggtgaaggt
      721 cacggcgaag agcccggact acgccgacgg gagcgaggtc gccgaggtga aggagacggg
      781 aggcaccgtc gaggtgaagc tcgtctcggg cggaagcgtc agcggcgtcg tcgtcgcgaa
      841 cggccagccc gtcccgggcg cgaacgtctc gctctcggcc gcgggggagg ccgggttcgg
      901 gcggatcctc ggcggcgggc agacgaccac gtccgacgcg accggccgct tcacgttcga
      961 gcacctcacg accggccgct acacggcgtc cgcggggctg aacggcaagt cgagcaacct
     1021 cgccgacgtc gtcctgcagc cgggcgactc gcgaaacgac gtcgtcctga cgctgtcggc
     1081 cggcacgtcc atccagggcg tcgtgagcgg cctgccggac ggctggcact ctcagacgac
     1141 cgtcatcgcg acgggcgtcg agtcgttctt cgcgacgacg cgcgtcggca acgacggctc
     1201 gttccgcgtc gacggcgtcc ccgcgggccc cgtgacgctc cgcgcgcagg cgggcgacgg
     1261 cctcgggacg tcgcgcagcg cttcgaagca ggtgaccgcg tccgacgacg tcccggtcct
     1321 ccagacggag atcgtcttcg agcagggctt cacgctgtcc gggcgcgtcg tccgcgcggg
     1381 ccagccggtc ccgaacgcgg tcgtcgccgc gaacctccag ggcgccggcg ggcgccagtc
     1441 cacgacgcgc tcggacgagt ccggcgcgta cgcgctgctc gggctccagg agggcgcgta
     1501 ctccgtgacc gcgagcggcg acccgctcgc gggcggagtc tcgatggtcc gtcagaccgt
     1561 caacctcacc ggcgaccaga gtctcgacct cacgttcccg acggcgcgcg tcggcggcgt
     1621 cgtctcggac gcgaccggca agcagccgct cgcggacgtg tccgtgacgc tcgcctccgc
     1681 ggcgggcgcg gcgacgccga tgcagcgcat gacgacgacg gactcgacgg gccgcttcca
     1741 gttcaccgac gtcgccccgc aggcctacac gctgaacgcg tcgaagccgg actaccagtt
     1801 cgacaagcgc accgtgaacg ccgccgacga cggctcggcg gacgcgctcg cgatcgagct
     1861 ccagcgcggg caggggatcg agctccgggt ccgcgacggc ctcgccgggg tcccgctccg
     1921 gagcgtcacc gtgcgcgtca cggacggcac gggcgcgacc gtctccgcgg ggtcgatcgc
     1981 gctcgattcg aacggcgtcg gcgacgtcac gtcgctcaag ccgggctcgt acaccgtcta
     2041 cgcgagcgcg aacggctacg cgaccgtcac gctcacggga atctcggtcc cgtcgcagac
     2101 gatcccggtg acgctcacgc cgggcggcgc ggcggacatc acggtcgggc cgaagtcgtt
     2161 cgtcaagggc gtcctccgcg ggacgctccg gaccgcggcc ggaacgccgt acccgtacca
     2221 gctcttcgcg acggacgggc ggctcacgat caacgccgac gcctcggggc agacgggctt
     2281 ccgccgtctc tcgaacctcg cgccggggag ctacgtcctc gcgctcgacg gcggcggcgg
     2341 gacgacgttc acgatcgccg agggagggat cacgccggtg cagctcccgt agcggatcgg
     2401 gccgcccggg ccgccccgta gaatg
//
LOCUS       DAOMFY010000416         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_20826_length_2414_cov_5.741416, whole genome shotgun sequence.
ACCESSION   DAOMFY010000416 DAOMFY010000000
VERSION     DAOMFY010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20826_length_2414_cov_5.741416"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /gene="argS"
                     /locus_tag="PLB01_19405"
     CDS             <1..1708
                     /gene="argS"
                     /locus_tag="PLB01_19405"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQQ79521.1"
                     /translation="NKAAHIGHLRNAVLGDVLVRNFRRLGRNVEVQNYLDDTGVQVAD
                     VVVALEHPDALVDPKKAGALGEEIREILAAFPYDAPGVPGRRLGDLAWDLYALVGKSY
                     AADPELEKARRDTLHAIEGAATGHDQTPAEKATAATAARLAEAVVRCHLLTMERIGVD
                     YDLLPRESDILARKFWVKAFERLRAAGAARLETEGKNAGCWVMPLEGAAEFEGMEDAD
                     KVLVRSNGTVTYTGKDVAYQLWKLGVLGIDFEYEPVPYVDVDGSPEFRASDGTSPLYR
                     TRHDAEGADASARGRFGKGTGVINVIDVRQSYPQKVVKEAVRKAGFPEAADRSVHFAY
                     EMVALTPASAEALGVELTDEDRAKPFVEMSGRKGLGVKADDLLDRLVEKARDQIASRA
                     GAERPEPGRAESIAVGALRVYMTRFSRNKIIAFDFDEALAFEGDTGPYLQYAAVRAAN
                     IFRKLEETGLAGRLDAAETAAVEALPLDTLDDGLWDVVRTCSRTLDTFEKVAETLEVS
                     LLVRHALAVAAAFHHLYHTHPISQEKDEARRRARRAALQVVSEHLADVLGVLGVPIPE
                     RM"
     gene            1705..2217
                     /locus_tag="PLB01_19410"
     CDS             1705..2217
                     /locus_tag="PLB01_19410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ79522.1"
                     /translation="MTQARDVRYRACGAADLPALAPVVARVFHEEFDTPADDAFAREA
                     EAASLIYDAHRDLCVAAEADGRAVGVLLVLPGGASGDAAVFTWLAVEGERRGSGIGRE
                     LLFRGIEACRERGRNLLRAYAYAVSPAACRLYWLYGFRVAGLEAFPVGGGVRERIVFE
                     KRLDPPATPA"
     gene            complement(2234..>2414)
                     /locus_tag="PLB01_19415"
     CDS             complement(2234..>2414)
                     /locus_tag="PLB01_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79523.1"
                     /translation="DGRIWVVDPASAARARFGEPGRGPRHVVVTAALDERTGVADVSD
                     VTEARSPAVSPAAVR"
BASE COUNT          337 a          900 c          868 g          309 t
ORIGIN      
        1 gaacaaggcc gcgcacatcg ggcatctccg gaacgccgtc ctcggcgacg tcctcgtccg
       61 gaacttccgg cgcctcgggc ggaacgtcga ggtccagaac tacctggacg acacgggcgt
      121 gcaggtcgcg gacgtcgtcg tcgcgctcga gcatcccgat gcgctcgtcg acccgaagaa
      181 agccggggcg ctcggcgagg agatccgcga gatcctcgcc gcgttcccgt acgacgcgcc
      241 gggcgtcccg gggcgccgcc tcggcgacct cgcctgggat ctctacgcgc tcgtcgggaa
      301 gagctacgcc gccgacccgg agctcgagaa ggcgcggcgc gacacgctcc acgcgatcga
      361 gggcgccgcg acgggccacg accagacgcc ggcggagaag gcgaccgcgg cgaccgccgc
      421 ccggctcgcc gaggccgtcg tgcgctgcca tctcctgacg atggagcgca tcggcgtcga
      481 ctacgacctc ctgccgcgcg aaagcgacat cctcgcccgc aagttctggg tcaaagcgtt
      541 cgagcgcctc agggccgccg gggccgcgcg cctcgagacc gagggcaaga acgcgggctg
      601 ctgggtcatg ccgctcgagg gcgccgccga gttcgaaggc atggaggacg cggacaaggt
      661 cctcgtacgc tcgaacggga ccgtcacgta cacgggcaag gacgtcgcct accagctctg
      721 gaagctgggg gtcctcggga tcgacttcga gtacgagccc gtcccgtacg tcgacgtgga
      781 cggcagcccg gagtttcggg cctcggacgg cacgtcgccg ctctacagga cgcgccacga
      841 cgccgagggc gcggacgcct cggcccgcgg ccgcttcggg aagggcaccg gcgtcatcaa
      901 cgtgatcgac gtccgccagt cctacccgca gaaggtcgtc aaggaggcgg tccggaaggc
      961 cggcttcccc gaggccgccg acaggtccgt ccacttcgcg tacgagatgg tcgcgctgac
     1021 gccggcctcc gccgaggcgc tcggcgtgga gctgacggac gaggatcgcg cgaagccgtt
     1081 cgtcgagatg tcgggccgca agggcctcgg cgtgaaggcc gacgaccttc tcgaccgcct
     1141 cgtcgagaag gcccgcgacc agatcgcctc ccgggcgggc gccgagcgcc cggagccggg
     1201 ccgcgcggag tccatcgccg tcggcgcgct gcgcgtctac atgacgcgct tctcgcgcaa
     1261 caagatcatc gcgttcgact tcgacgaggc gctcgcgttc gagggcgaca cggggccgta
     1321 tctccagtac gccgccgtgc gcgccgcgaa catcttccgg aagctcgagg agaccggcct
     1381 cgcgggacgg ctcgacgcgg ccgagacggc ggccgtcgag gcgctgcccc tcgacacgct
     1441 cgacgacggc ctgtgggacg tcgtccggac gtgctcgcgt acgctcgaca cgttcgagaa
     1501 ggtcgccgag acgctcgaag tctcgctcct cgtgcggcac gcgctcgcgg tcgccgcggc
     1561 gttccaccac ctctaccaca cgcacccgat ctcccaggag aaagacgagg cgcgccggcg
     1621 cgcgcgccgc gcagccctcc aggtcgtctc ggagcacctc gcggacgtcc tcggcgtcct
     1681 cggcgtcccg atcccggaga ggatgtgacg caggcgcgcg acgtccggta tcgcgcgtgc
     1741 ggcgccgcgg atctccccgc gctcgcgccg gtcgtggcgc gcgtcttcca cgaggagttc
     1801 gacacgccgg cggacgacgc cttcgcgcgc gaggccgagg cggcatcgct gatctacgac
     1861 gcgcaccgcg acctgtgcgt cgcggccgag gcggacggcc gcgccgtcgg cgttcttctc
     1921 gtcctgccgg gaggcgcgtc gggagacgcg gccgtcttca cctggctcgc cgtcgagggc
     1981 gagcggcgag ggtccggcat cgggcgcgag ctcctgttcc gcggcatcga ggcctgccgg
     2041 gagcgcggcc gcaacctcct gcgggcgtac gcctacgcgg tgtcccccgc cgcgtgccgc
     2101 ctctactggc tctacgggtt ccgcgtcgcg ggcctcgagg ccttccccgt cgggggagga
     2161 gtccgcgagc gcatcgtctt cgagaagcgg ctcgaccctc cggcgacgcc cgcctgacgc
     2221 gccggcgcgg ggctcagcgc accgcggccg gcgagaccgc gggcgaccgg gcttccgtca
     2281 cgtccgaaac gtccgcgacg cccgtccgct cgtcgagcgc ggccgtcacg acgacgtgcc
     2341 gcggcccgcg tcccggctcg ccgaaacgcg cgcgcgcggc gcttgcggga tcgacaaccc
     2401 agatccggcc gtcg
//
LOCUS       DAOMFY010000417         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_20858_length_2412_cov_6.239287, whole genome shotgun sequence.
ACCESSION   DAOMFY010000417 DAOMFY010000000
VERSION     DAOMFY010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20858_length_2412_cov_6.239287"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PLB01_19420"
     CDS             <1..1105
                     /locus_tag="PLB01_19420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQQ79524.1"
                     /translation="TGVAYLDLARAAEGDEPELSAAVLAAVRRGRYVLGSEVAAFESE
                     WAAFCGAARAVGVANGTDAITLALRALGVGPGDEVLTVSMTCAPTATGILRAGATPVF
                     VDVEEDRLTMDAGALAAAVTPRTKAILPVHLYGRVADVEAIGAFARQNDVLLVEDCAQ
                     AHGAAWNGRPAGTFGRAAAWSFYPTKNLGALGDGGAVTVVGEGDAAVADRVARLRVYG
                     YVTRNDAAEPGYNSRLDEVQAAALRVRLRKLSAGNRRRAAIAARYDAALAGAVRVPPP
                     AREGESPAHHLYVVRVPDRDAFRARLAGCGIGTDVHYPRAVHEQPAFASLPRGPLPVT
                     ERAMREVVSLPLDPHLTDAEADRVIEAVLASAR"
     gene            complement(1094..1882)
                     /locus_tag="PLB01_19425"
     CDS             complement(1094..1882)
                     /locus_tag="PLB01_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79525.1"
                     /translation="MRRNVLLAAAALALATLGCRRPPAPYGTHTAFVPVVFRSNNPGR
                     PPWAPRVTLTNTSTTSAAVRLTRWPPDAPEAEQRLFVLAPGQTVSVPGRTPLGAASSL
                     FFEGKSPFLVRAEIVDRRRIAPPLSVPILKAEDLARPGDRLTLGPIVDTPAERSHFCF
                     TYPGVERDAVPFRIHLRLTAPGGGALLWEGTFVLTGLPLVIDDPWKRFHLAPGTAFDV
                     DVAFLGSARSRPVARGMWVYGITTDRASGASRFLETRVARGSAR"
     gene            complement(1879..>2412)
                     /locus_tag="PLB01_19430"
     CDS             complement(1879..>2412)
                     /locus_tag="PLB01_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79526.1"
                     /translation="REAGHSAGRAAARAAFAGASPLAGFGIFCLYFGRRFGDPLLFVK
                     AQHNWSGQSKSILDGPALIWQAVAEDVTRGRLLGGSPARTFEGLYLLLFLVLAAVLLA
                     QKRRPEALYVALTVAIVFSSGTFESAGRYVLPAFPAFAALAGLSARRGFFGALVALSS
                     VAGALYVWAFVHWYWAG"
BASE COUNT          350 a          852 c          936 g          274 t
ORIGIN      
        1 gaccggcgtc gcgtatctcg acctcgcgcg ggccgccgaa ggcgacgagc cggagctttc
       61 cgccgcggtg ctcgccgccg tacgccgcgg gcgctacgtc ctcggcagcg aggtcgccgc
      121 gttcgagtcc gagtgggccg ccttctgcgg cgccgcgcgc gcggtcggag tcgcgaacgg
      181 caccgacgcg atcacgctcg ccctgcgcgc gctcggcgtc gggccgggcg acgaggtcct
      241 gaccgtttcc atgacgtgcg cgcccaccgc gacggggatc ctccgcgcgg gcgcgacgcc
      301 cgtcttcgtg gacgtcgagg aggatcgcct cacgatggac gccggcgcgc tcgcggccgc
      361 cgtcacgccg aggacgaagg cgatcctccc ggtccacctc tacggacgag tcgcggacgt
      421 cgaggcgatc ggcgcctttg cgcggcagaa cgacgtcctc ctcgtcgagg attgcgcgca
      481 ggcgcacggc gccgcgtgga acggccgccc cgcgggaacg ttcggccgcg ccgcggcgtg
      541 gagcttctac ccgacgaaga acctcggggc gctcggcgac gggggcgcgg tgacggtcgt
      601 gggcgagggc gacgcggccg tcgcggatcg cgtcgcgcgc ctcagggtct acgggtacgt
      661 gacgcgaaac gacgccgcgg agcccgggta caacagccgg ctcgacgagg tccaggcggc
      721 ggcgttgcgg gtccggctgc gcaagctctc cgccggcaac cgccggcgtg cggcgatcgc
      781 ggcccgctac gacgccgccc ttgcaggcgc cgtgcgcgtc ccgcccccgg cccgggaggg
      841 cgaatcgccg gcgcaccatc tctacgtcgt gcgcgtgccg gaccgggacg ccttccgggc
      901 gcgtctcgcc gggtgcggga tcggaacgga cgtccactac ccgcgcgccg tgcacgagca
      961 gcccgcgttc gcgtctcttc cgcgcggacc gctcccggtc accgagcgcg cgatgcggga
     1021 ggtcgtctcg ctcccgctcg acccgcacct cacggacgcg gaggcggacc gggtgatcga
     1081 ggccgtgctc gcgtcagcgc gctgagccgc gggcgacccg cgtctcgaga aaacgcgagg
     1141 cgccgctcgc gcggtccgtg gtgatcccgt agacccacat gccgcgggcg accggccggc
     1201 tgcgcgcgct cccgaggaac gcgacatcga cgtcgaacgc ggtgcccggc gcaaggtgga
     1261 agcgcttcca cgggtcgtcg atcacgaggg gcagccccgt gaggacgaac gtcccctccc
     1321 acaggagggc gccgccgccg ggcgccgtca gccggaggtg gatgcggaag ggcacggcgt
     1381 cgcgctcgac gccggggtac gtgaagcaga agtggctccg ttccgcgggc gtgtcgacga
     1441 tcggcccgag ggtgaggcgg tcgcccggcc gcgcgaggtc ttccgccttc aggatcggga
     1501 cggaaagcgg cggtgcgatc cggcgccggt ccacgatttc ggcgcggacg aggaacgggg
     1561 attttccctc gaagaacaga gaggaggcgg cgccaagggg ggtgcgcccg ggcacggaga
     1621 cggtctgccc cggcgcgagc acgaaaagcc tctgctcggc ctccggtgcg tccggcggcc
     1681 agcgggtcag ccgcaccgcg gcggacgtcg tggacgtgtt cgtcagggtc actctcggcg
     1741 cccagggcgg acggccgggg ttgttcgagc ggaacacgac cggcacgaac gccgtgtgcg
     1801 tgccgtaagg cgccggcggc cggcggcaac cgagtgtcgc gagagcgagc gcggcggcgg
     1861 ccaggaggac gttccttctc atccggccca gtaccagtgg acgaaggccc acacgtagag
     1921 ggcaccggcg acggagctca gcgccacgag cgcgccgaag aacccgcggc gcgcggagag
     1981 accggccagg gccgcgaagg cggggaacgc gggcaggacg tagcggccgg cgctctcgaa
     2041 cgtgccggac gagaagacga tcgcgacggt caaggccaca tagagggcct ccggcctccg
     2101 cttctgcgcg aggaggaccg cggcgaggac gaggaagaga aggagataga gcccttcgaa
     2161 cgtccgtgcg ggcgagcctc cgagcaggcg gccgcgcgtc acgtcctcgg cgacggcctg
     2221 ccagatgagg gccggcccgt ccaggatcga cttggactgg ccgctccagt tgtgctgagc
     2281 cttcacgaag aggagcgggt cgccgaaacg gcgtccgaaa tagagacaga agatgccgaa
     2341 gcccgcgagc ggcgaggcgc ccgcgaacgc cgcgcgcgcc gcggcccggc cggccgaatg
     2401 cccggcctcg cg
//
LOCUS       DAOMFY010000418         2407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_20952_length_2407_cov_5.268282, whole genome shotgun sequence.
ACCESSION   DAOMFY010000418 DAOMFY010000000
VERSION     DAOMFY010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20952_length_2407_cov_5.268282"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLB01_19435"
     CDS             complement(<1..458)
                     /locus_tag="PLB01_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQQ79527.1"
                     /translation="MIGSVKGTRDLLPPETRVWNRVEAVAREVFGLYGYDEIRTPVLE
                     STELFVRSVGEGTDIVHKEMYTFTDRGDRSVTLRPENTAGVARAYVEHSLAQAGRPWK
                     LFYIGPQFRYERPQKGRYREFRQIGAEVLGPADAATDAEVLVMLFDFLGRL"
     gene            complement(455..967)
                     /locus_tag="PLB01_19440"
     CDS             complement(455..967)
                     /locus_tag="PLB01_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQQ79528.1"
                     /translation="MVLRSYLGVAPKLGERVFIAETAAVIGDVVLGDDVSIWYSAVVR
                     GDCCHIRIGARSNVQDNCTLHVTRDTGPLVLEEEVTLGHNAVVHACTIRRGALIGMNA
                     TVLDDADVGRSALVAAGAVVLPGTVIPPATLWAGSPAKKKRDLTPDEVVGLETYWKNY
                     LVYKAEYLKP"
     gene            complement(1209..>2407)
                     /locus_tag="PLB01_19445"
     CDS             complement(1209..>2407)
                     /locus_tag="PLB01_19445"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HQQ79529.1"
                     /translation="APAGDLKAVLAWMKANPGKVNYATSGAGTVPHFAGQMLAQAVAA
                     LEDDEAGALRAAGAARAALLSLEEISPSYAARAAMLAEAIERLREVGFETSRDLAQLE
                     ADPARLAFIAERLDALAKLKRKYGATLAEVIERRAALGKEREELADLEGAVKKAEAAA
                     QKAFAAFRKSALELSQERAAAAPRLGKAVESHLTDLALRRAAFAVEVARKPDPESAFA
                     AGGENVAFGAHGIDAVAFSFAPNPGEPPRPLPKIASGGELSRVQLALAAALAEEEARG
                     ARSGGGDRRGGPVRTFVFDEVDAGVSGATADAVGRKLRALAAREQILVVTHLPQVAAA
                     GTHHLAVEKVAAAGRTRTRVTALDAKGRVEAVAALLAGASVGEPARAQARMLLDGASR
                     GVSKAR"
BASE COUNT          342 a          867 c          835 g          363 t
ORIGIN      
        1 aggcgcccga ggaagtcgaa gagcatgacg aggacttcgg cgtcggtcgc ggcgtccgcg
       61 ggcccgagca cctccgcgcc gatctgccgg aactcgcggt accggccctt ctgcggcctc
      121 tcgtaccgga actgaggccc gatgtagaag agcttccacg ggcggcccgc ctgcgcgaga
      181 gagtgctcga cgtacgcgcg ggcgacgccc gccgtgttct ccggccgcag cgtcacggat
      241 cggtcgccgc ggtccgtgaa cgtgtacatc tccttgtgga cgatgtccgt cccctcgccg
      301 acgctgcgca cgaagagctc ggtcgactcg agcaccggcg tgcggatctc gtcgtagcca
      361 tacagcccga agacctcgcg ggcgacggct tccacccggt tccagacacg ggtctcgggc
      421 gggaggaggt cccgcgtccc cttcacgctc ccgatcacgg cttgaggtac tccgccttgt
      481 agacgaggta gttcttccag tacgtctcga gccccacgac ctcgtccggc gtgaggtctc
      541 gcttcttctt cgcggggctg ccggcccaga gcgtcgcggg agggatcacc gtgcccggga
      601 ggaccaccgc tccggccgcg acgagggcgc tccggccgac gtcggcgtcg tcgaggaccg
      661 tcgcgttcat gccgatcagc gcgccgcgcc ggatcgtgca cgcgtggacg accgcgttgt
      721 gcccgagcgt gacctcctcc tcgaggacga gggggcccgt gtctcgcgtg acgtgcagcg
      781 tgcagttgtc ctgaacgttc gatcgcgctc cgatgcggat gtggcagcag tcgccgcgca
      841 cgacggccga gtaccagatc gagacgtcgt ccccgaggac gacgtctccg atgacggcgg
      901 cggtctccgc gatgaagacc cgctcgccga gtttcggggc gactccgaga tagcttcgca
      961 gcaccatttc gccggtcacc ataccaaacg aagggccgtg aaacgcgcgc tgcacagacc
     1021 tctgtgcgag gcggataaaa ccgcgtgaag ttgcggggtt gcggcccttt tcacgcccca
     1081 atgcacaggc ttttgcacag aaacggtcta atactccaag tgaggttttc acagatactt
     1141 acgcggttcg tatcgcgaat atgggccgag ttgtagaccg gtggcagtcg ggggtctgtg
     1201 cgaagccgtc agcgcgcctt cgagacaccc cgggaggccc cgtccaggag catacgggcc
     1261 tgtgcgcggg ccggctcgcc gaccgaagct ccggccagga gggccgcgac ggcctcgact
     1321 cttcccttcg cgtccagggc tgtgacccgg gtccgcgtcc ggcctgccgc ggccaccttc
     1381 tcgacggcga ggtggtgcgt gcccgccgcc gcaacctggg gcagatgcgt cacgacgagg
     1441 atctgctcgc gggcggcgag cgcgcgcagc ttccggccga ccgcgtccgc ggtggcgccc
     1501 gagacgcccg catcgacctc gtcgaagacg aacgtccgga ccggccctcc gcgccggtcc
     1561 ccgccgccgg accgggcccc ccgggcttcc tcctccgcga gcgccgccgc gagagccagc
     1621 tggacgcggg aaagctcgcc tccggacgcg atcttcggga gcgggcgcgg cggctcgccc
     1681 gggtttggcg cgaaggagaa cgcgacggcg tcgatcccgt gggcgccgaa ggcgacgttc
     1741 tcgccgcccg ccgcgaaggc gctctccgga tcgggcttgc gcgcaacctc gacggcaaac
     1801 gccgcccggc ggagcgcgag gtcggtcagg tgggactcga cggcctttcc cagccgcggc
     1861 gcggcggcag cccgctcctg cgaaagctcg agcgccgact tccggaaggc cgcgaacgcc
     1921 ttctgggccg ccgcctcggc cttcttcacg gcgccctcca ggtcggccag ctcctcgcgc
     1981 tccttcccga gagccgcgcg gcgctcgatc acctcggcca gcgtcgcgcc gtacttgcgc
     2041 ttgagtttcg cgagcgcgtc gaggcgctcg gcgatgaacg caaggcgcgc gggatcggcc
     2101 tcgagctgcg cgagatcgcg ggaagtctcg aacccgacct cccgaagcct ctcgattgcc
     2161 tccgcgagca tcgcggctcg tgcggcgtac gaaggggaga tttcttcgag tgaaagaagg
     2221 gcggcgcgcg ccgcgccggc cgcgcgaagg gcgcccgcct cgtcgtcctc cagcgccgcg
     2281 acggcctgcg ccagcatctg gcccgcgaaa tgcggcaccg tgcccgcccc ggacgtggcg
     2341 tagttgacct tgccggggtt ggccttcatc cacgccagca cggccttcag gtcgcccgcg
     2401 ggggcgc
//
LOCUS       DAOMFY010000419         2404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_20988_length_2404_cov_8.505321, whole genome shotgun sequence.
ACCESSION   DAOMFY010000419 DAOMFY010000000
VERSION     DAOMFY010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20988_length_2404_cov_8.505321"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..686)
                     /locus_tag="PLB01_19450"
     CDS             complement(198..686)
                     /locus_tag="PLB01_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQQ79530.1"
                     /translation="MKTVTKSLIAAGVVLALASPVQAGEKKDIVDTAVAAGSFTTLAK
                     ALGAAGLVDTLKGTGPFTVFAPTDEAFAKLPAGTLDKLLADKAQLSKVLTYHVVAGKV
                     MAADVVKLKSAKTVEGEDVKITVKDKSVMVNQAHVVKTDIETSNGVIHVIDAVLLPPA
                     KK"
     gene            839..1384
                     /locus_tag="PLB01_19455"
     CDS             839..1384
                     /locus_tag="PLB01_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQQ79531.1"
                     /translation="MKFALIAAAALAAGAALAAGDAALYGFTMKTIDGKPKALGDYRG
                     KALLIVNTASECGYTPQYAGLEKLYERYRDRGFMVLAFPANDFGAQEPGSDAQIKQFC
                     SSRYGTQFDLFSKISVKGKEQHPLYAYLTSVKGFEGEIRWNFNKFLVDPSGTVVARYA
                     SGVEPTSAELTAKLESILPRN"
     gene            complement(1401..>2404)
                     /locus_tag="PLB01_19460"
     CDS             complement(1401..>2404)
                     /locus_tag="PLB01_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ79532.1"
                     /translation="GDKKGRLSTLFGSIALYSVANIANAFVHGIPSYAVWRFLAGLGL
                     AGELGAAVTLVAEILPRNLRAYGTAIVAGVGIFGTVTASLVGKYLPWRTAYLVGGGLG
                     LVLLATRFSLRESAMFKTLGEQTGVTRGDFLSLFTSGERFLRYLRCILIGLPTWFVVA
                     ILITSAPEFAPKVGVTGPVTAGTAVAFCYSGITLGSFASGILSQAWGSRKKVVLLFIL
                     GTLAGVAAYLTLRGLSPIAFYFLAGFLGLMAGYWAVFVTIAAEQFGTNLRATVATTVP
                     NFVRGAVPLITGGFSLLRAPLGYVGSAWAVGLACIALALVSLKGMAETAGRDLDFLE"
BASE COUNT          402 a          836 c          805 g          361 t
ORIGIN      
        1 cgcctgcggc cgcgccggcg cgcaccagtt cgacgcggtc cccgagcgtt cccgtgcatc
       61 cggatgtcgc gccgcggagt aagcccgtcg gcgtcggccg ccgcccgggc tgagggagga
      121 gctcgcgcct cctcgcctgc tcacgaaaaa gggccggcgc agaagcgccg gccccctcac
      181 cgagagattt cgcccgatta cttcttcgcc ggcggcagaa gcacggcgtc gatcacgtgg
      241 atgacgccgt tcgacgtctc gatgtccgtc ttgaccacgt gcgcctgatt caccatcacg
      301 ctcttgtcct tgacggtgat cttgacgtcc tctccctcca ccgtcttggc cgacttcagc
      361 ttcacgacgt ccgccgccat gacctttccg gcgacgacgt ggtacgtcag gaccttgctc
      421 agctgcgcct tgtcggccag cagcttgtcc agggtgcccg cgggcagctt cgcgaaggcc
      481 tcatccgtcg gcgcgaagac ggtgaacggg cccgtgccct tcagcgtgtc gacgagccct
      541 gcggcgccga gcgccttggc gagcgtcgtg aacgagcccg cggccacggc cgtatccacg
      601 atgtccttct tctcgccggc ctgaacgggg gacgcaagcg ccagaacgac tccggcggca
      661 atcaacgact tggtgacggt cttcatggga ttctccttct tgttggcggc gaactcgcgc
      721 cgtgccctga ttacggcccg ctctcgagga cggattcgag gcgcacgtcc aaagacgtaa
      781 cgccgtgtgc cgcaaaccgc tgcgccgccg ccagcgtaga gaccccgtga ggttatccat
      841 gaagttcgcg ctcatcgccg ccgccgccct cgccgcgggt gccgcactcg ccgccggcga
      901 cgccgcgctt tacggcttca cgatgaagac gatcgacggc aagccgaagg cgctcggcga
      961 ctaccgcggc aaggcgctcc tcatcgtcaa cacggcctcc gaatgcggtt acacgcccca
     1021 gtacgccggc ctcgagaagc tctacgagcg ctatcgcgac cggggcttca tggtcctcgc
     1081 cttccccgcg aacgacttcg gcgcgcagga gcccggctcc gatgcccaga tcaagcagtt
     1141 ctgctcgtcc cggtacggga cgcagttcga cctcttctcc aagatctcgg tcaaggggaa
     1201 ggagcaacat ccgctgtacg cgtacctcac gtccgtcaag ggattcgagg gagagatccg
     1261 gtggaacttc aacaagttcc tcgtcgatcc ctccggcacg gtcgtcgcgc gctacgcgtc
     1321 aggcgtcgag ccgacgtccg ccgagctcac ggcgaagctc gagtcgatcc tcccgaggaa
     1381 ctgaacccgc tcgctttccg tcactccagg aagtcgaggt cgcgccccgc cgtctcggcc
     1441 atgcccttga gggagaccag ggcgagggcg atgcaggcga ggcccaccgc ccaggcgctg
     1501 cccacgtagc ccagaggggc gcggagcagg gagaagccgc cggtgatcag ggggacggct
     1561 ccgcgtacga agttcggcac cgtggtcgcc acggtggcgc gaaggttcgt gccgaactgc
     1621 tccgcggcga tcgtgacgaa gacggcccag tagcccgcca tcaggccgag gaatcccgcg
     1681 aggaagtaga acgcgatcgg gctgagcccg cgcagcgtga ggtaggccgc gacgccggcg
     1741 agcgtcccga ggatgaagag aagaacgacc ttcttgcggc ttccccaggc ctggctgagg
     1801 atgccggacg cgaagctccc gagcgtgatg ccgctgtagc agaaggccac ggcggtgccc
     1861 gcggtcacgg gccccgtcac gccgaccttc ggcgcgaact cgggcgcgga cgtgatgagg
     1921 atcgccacca cgaaccacgt cggcaggccg atgaggatgc agcggaggta gcgcaggaag
     1981 cgctccccgc tcgtgaagag gctcaggaag tcgccccgtg tcacgcccgt ctgctcgccg
     2041 agcgtcttga acatcgcgct ctcgcggagg ctgaagcggg tggcgaggag gacgaggccg
     2101 agcccgccgc cgacgagata ggcggtgcgc cacgggaggt acttgccgac gaggctcgcg
     2161 gtcaccgtgc cgaagatgcc gacgcccgcc acgatggcgg tgccgtaggc ccggaggtta
     2221 cgcgggagga tctcggcgac gagcgtcacc gccgcgccca gctcgcccgc gagaccgagc
     2281 ccggccagga accgccagac ggcgtaggac gggatcccgt gcacgaaggc gttcgcgatg
     2341 ttcgccacgg agtagagcgc gatgctcccg aagagcgtcg agaggcggcc cttcttgtcg
     2401 ccga
//
LOCUS       DAOMFY010000420         2388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_21262_length_2388_cov_7.673811, whole genome shotgun sequence.
ACCESSION   DAOMFY010000420 DAOMFY010000000
VERSION     DAOMFY010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21262_length_2388_cov_7.673811"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PLB01_19465"
     CDS             <1..1010
                     /locus_tag="PLB01_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79533.1"
                     /translation="HDLTALVRGAVRLAADPAALPPPGLSEDGAGRLAGFARVTPYQT
                     LLRLLTLLSESDGTLRRSDVPALAFEVLLLRLAELPKLVGIEEFLSGKTPVPSGSTSP
                     GGSGGAGAGGGGGFSSKVSEAAPRADAGSRASDAPAASGHSPSKKSFAAAAAAPPGAA
                     MGSRSGAPSEAEDSSSSEDVPRFVGLTPLEIIPEPLDTPDPAGAFRAAVEKKNSQLGA
                     VLEDTSITLEGKVVRILLDPPSTIVEKRLAEPAVLRVLEDAALKVVGRGARVVVENAS
                     PPGGDLTAAAVAADPAALEQEHLRHKVASDERVKRMMDLFGGEIADVRRDDGSGRPKG
                     R"
     gene            1011..1619
                     /gene="recR"
                     /locus_tag="PLB01_19470"
     CDS             1011..1619
                     /gene="recR"
                     /locus_tag="PLB01_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQQ79534.1"
                     /translation="MASLPALDRLVAALEKLPGIGPKSARRIAHFLLAAPEFDAAVLT
                     SAVTEARAKTRACGVCHALTEDDPCSVCADPARDARVLAVVEEAFDVDVLERTHEFRG
                     RYHVLGGALAPLRGIGPDDLHVAELVARVEKAAPDARIEEVLIATNPNVEGEATALYL
                     ARRLKPLGVRVTRLALGLPVGAALEFADDVTLSRSLAGRREM"
     gene            1643..2137
                     /locus_tag="PLB01_19475"
     CDS             1643..2137
                     /locus_tag="PLB01_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HQQ79535.1"
                     /translation="MSDFVLFDEEFHQIKDVIGRLKVDAQAKVVFIVDKNGQQIAAMG
                     DMDSLDTTSLASLTAGNVAATDGLARLIGEKEFPVLFHEGERDNVYISIVGQRVILVV
                     IFDERSSLGLVRLRVRKANAELERVFETIQKKVESEKVRRQEGYDSPFAEITDEDIDS
                     LFRE"
     gene            2147..>2388
                     /locus_tag="PLB01_19480"
     CDS             2147..>2388
                     /locus_tag="PLB01_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HQQ79536.1"
                     /translation="MTFINYAAREINCKIVYYGPGLCGKTTNLQYIYDRTNPAAKGKL
                     ISLATETDRTLFFDFLPLDLGTVRGFKTRFHLYTVPG"
BASE COUNT          362 a          864 c          792 g          370 t
ORIGIN      
        1 tccacgacct cacggccctc gtccgcggcg cggtgcgcct cgcggccgac ccggctgcgc
       61 tgccgccccc cgggctctcc gaggacggcg ccggccgcct cgccgggttt gcgcgcgtga
      121 cgccgtacca gacgctcctg cgcctcctga cgctcctctc cgagtccgac ggcacgctgc
      181 gccggtccga cgtccccgcg ctcgcgttcg aagtgctcct cctgcgcctc gcggagcttc
      241 cgaagctcgt cggaatcgaa gagtttcttt ctggtaagac tcccgtgcct tccggctcga
      301 cgtcgccggg cggctccggt ggagcagggg ctggcggtgg agggggattt tcttcgaagg
      361 tgagtgaggc ggcgccgcgc gccgatgccg gctcgcgcgc aagtgacgcc cccgctgcct
      421 ccggacattc accttctaag aaatctttcg ccgccgcggc ggctgctccg ccgggcgccg
      481 cgatgggcag ccggtccggt gcgccgtccg aagcagaaga ttcttcttct tccgaagatg
      541 ttccccgctt cgtgggcctc acgccgctcg agatcatccc cgagccgctc gacacgcccg
      601 accccgcggg cgcgttccgc gcggcggtcg agaagaagaa ctcgcagctc ggcgcggtcc
      661 tcgaggacac gtccatcacg ctcgagggca aggtcgtgcg gatcctcctc gacccgccga
      721 gcacgatcgt cgagaagcgc ctcgccgaac cggccgtcct ccgcgtcctc gaggacgccg
      781 cgctcaaggt cgtgggccgc ggcgcgaggg tcgtcgtcga gaacgcctcg cctccgggcg
      841 gcgacctcac ggccgcggcg gtggccgcgg accccgcggc tctcgaacag gagcacctcc
      901 ggcacaaggt cgcctccgac gagcgcgtga agaggatgat ggatctcttc ggcggcgaga
      961 tcgcggacgt caggcgcgac gacggctccg gccggcccaa gggtcgctga gtggcgtccc
     1021 tcccggccct cgaccgtctc gtggcggctc tcgagaagct gcccggcatc ggcccgaaga
     1081 gcgcccggcg gatcgcgcac ttcctgctcg cggcgccgga gttcgacgcg gcggtcctga
     1141 cgtccgcggt gacggaggcc cgggcgaaga cgcgcgcgtg cggcgtttgc cacgccctca
     1201 cggaggatga tccctgctcg gtctgcgccg acccggcgcg tgacgcgcgc gtcctcgcgg
     1261 tggtcgagga ggcgttcgac gtggacgtcc tcgagcgcac gcacgagttc cgggggcgat
     1321 accacgtcct cggaggggcc ctcgcgccgc tccggggcat cggcccggac gacctccacg
     1381 tggccgagct cgtcgcacgg gtcgagaaag ccgctcccga cgcccggatc gaggaggttc
     1441 tgatcgccac gaacccgaac gtcgaggggg aggccaccgc cctctacctg gcccgccggc
     1501 tcaagccgct cggggttcgc gtgacgcggc tcgccctcgg cctcccggta ggcgcggccc
     1561 tggaattcgc cgacgacgtg acgctctccc gatcgctcgc gggccgccgg gagatgtgat
     1621 cgggtaaact gtcccgaaac gcatgtccga tttcgttctc ttcgacgagg agttccatca
     1681 gatcaaggac gtcatcgggc gtctgaaggt ggacgcccag gcgaaggtcg tcttcatcgt
     1741 cgacaagaac gggcaacaga tcgccgccat gggcgacatg gattccctgg acacgacgtc
     1801 cctcgcctcg ctgacggccg gaaacgtcgc ggcgacggac ggcctcgcgc gcctgatcgg
     1861 cgagaaggaa ttcccggtcc tgttccacga gggcgagcgc gacaacgtct acatctcgat
     1921 cgtcggccag cgcgtgatcc tcgtcgtcat cttcgacgag cgctcctcgc tcgggctcgt
     1981 gaggctccgg gtgcgcaagg cgaacgccga gctcgagcgc gtcttcgaga ccatccagaa
     2041 gaaagtggag tccgaaaaag tccgcaggca ggaaggctac gactcgccct tcgccgagat
     2101 caccgacgaa gacatcgaca gccttttccg ggagtagcgc agctcgatga cgttcatcaa
     2161 ctacgcagcc cgcgagatca actgcaagat cgtctattac ggccccggcc tctgcggcaa
     2221 gacgacgaac ctgcagtaca tctacgaccg cacgaatccc gcggcgaaag ggaagctcat
     2281 ctccctcgcg acggagaccg accgcacgct cttcttcgac ttcctcccgc tcgacctcgg
     2341 gacggtgcgg ggcttcaaga cgcggttcca cctctacacg gtccccgg
//
LOCUS       DAOMFY010000421         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_21564_length_2369_cov_6.433449, whole genome shotgun sequence.
ACCESSION   DAOMFY010000421 DAOMFY010000000
VERSION     DAOMFY010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21564_length_2369_cov_6.433449"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..356)
                     /locus_tag="PLB01_19485"
     CDS             complement(30..356)
                     /locus_tag="PLB01_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79537.1"
                     /translation="MGHTNLRARILAYLEGVVTGNLLRSEREIASAVAATVEEVHETI
                     RDLVRRGEVFEPIPRRYLRRERRIGERRGVAEQAAQAALAIVDAVAGDDLLRREARAV
                     ILDVDA"
     gene            408..869
                     /locus_tag="PLB01_19490"
     CDS             408..869
                     /locus_tag="PLB01_19490"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQQ79538.1"
                     /translation="MPTPIRSLTLAAVALALAGCASAPPAAPAHYPSSPATLPFSEAV
                     RDGDLLFVSGQIGNPPGKLELVPGGLRAEAAQALENIRAIVERHGSSMTQVVKCTVFL
                     ADMKEWGDFNEVYRKAFPGKLPARSALGANGLALGARVEVECIAHVPQEKR"
     gene            872..1198
                     /locus_tag="PLB01_19495"
     CDS             872..1198
                     /locus_tag="PLB01_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQQ79539.1"
                     /translation="MSSVPLPPGPVSLPDLVAFQPGAVVSRTLVKTGGGTVTAFAFDA
                     GEALSEHSAPFDALVIGVEGEAEVSISKVPHRVKAGDLLRLPANEPHGLKAVTPFKMI
                     LVMVKA"
     gene            1195..1542
                     /locus_tag="PLB01_19500"
     CDS             1195..1542
                     /locus_tag="PLB01_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79540.1"
                     /translation="MTVKIVAALAAAAALSGCGSFAEQDDAPARIEAALQAWAKTEGK
                     EMKGGSLQIFGPSALQNGTFRQKNRLPDGTVVETQGTFEAEWDRQPDGSWLVARMTIN
                     PPPFTPPDAGSVP"
     gene            1539..2174
                     /locus_tag="PLB01_19505"
     CDS             1539..2174
                     /locus_tag="PLB01_19505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ79541.1"
                     /translation="MSAGRAKVYDHAYFQTWYRDPKKAIVRRADLERKVAMALGAAEH
                     LLGRTVDSVLDVGCGEGSWQPILRRLRPRLKYLGLDTSEYAIRRYGRSRNLRAGSWGQ
                     LSELRFGGPFDLVVCSDALHYVPTKELLRGLGGLPALVGGAAYLDFLTSDDDLETGLE
                     GDLVGFRKRTAAWYRKKLRATGLVECGLGIWAAAELAPALTAMERGPKQPR"
BASE COUNT          340 a          860 c          830 g          339 t
ORIGIN      
        1 ccgtgcgtct ccacccgtcc gagggcagcc tatgcgtcga cgtcgaggat gacggccctg
       61 gcttcacgcc gcaggagatc atctccggcc acggcatcga caatcgccag ggccgcctgc
      121 gcagcctgtt cggcgacgcc gcgtcgctct ccaatgcgcc gctcgcggcg gaggtaccgc
      181 cggggtatcg gctcgaacac ctcgccgcgg cggacgaggt cgcggatcgt ctcgtggact
      241 tcctcgaccg tcgccgcgac ggcggaggcg atctcgcgct cgctccgcag gaggttgccc
      301 gtcacgactc cttcgaggta cgccaggatc cgggcacgca ggttcgtatg gcccatggcc
      361 gcggactata tcgccgtgcc gtcgtaagct gccccccgga ggtctcgatg cccaccccga
      421 tccgatccct cacgctcgcc gccgtcgcgc tcgccctcgc aggctgcgcg tccgctccgc
      481 cggcggcgcc cgcccactac ccgtcgtcgc ccgcgacgct gcccttctcg gaggccgtcc
      541 gtgacggcga tcttctcttc gtgtcgggac agatcggcaa tccgccgggc aagctcgagc
      601 tcgtgcccgg cggcctgcgc gccgaggccg cgcaggcgct cgagaacatc cgcgcgatcg
      661 tcgagcgaca cggctcgagc atgacccagg tcgtgaagtg caccgtgttc ctcgccgaca
      721 tgaaggaatg gggcgacttc aacgaggttt atcgcaaggc cttccccggc aagctccccg
      781 cgcgcagcgc gctcggcgcg aacggtctcg cgctcggcgc gcgcgtcgag gtggagtgca
      841 tcgcccacgt cccgcaggag aagaggtagc catgtcgagc gtcccgcttc cccccggacc
      901 cgtctccctg cccgacctcg tggccttcca gccgggagcc gtcgtgagcc gcacgctcgt
      961 caagacgggc ggcggcaccg tgacggcctt cgcgttcgac gcgggcgagg cgctctccga
     1021 gcactcggcg cctttcgacg cgctcgtgat cggcgtcgag ggcgaggccg aggtctcgat
     1081 ctcgaaggtc ccgcaccgcg tgaaggcggg cgacctcctg cgcctcccgg cgaacgaacc
     1141 gcacgggttg aaagcggtca cgcctttcaa gatgatcctc gtcatggtga aagcgtgacg
     1201 gtgaagatcg tggcggccct cgcggccgcg gcggcgctgt cgggctgcgg ctcgttcgcg
     1261 gaacaggacg acgctcccgc gcggatcgag gcggcgctgc aggcctgggc gaagaccgaa
     1321 gggaaagaga tgaagggggg ctccctgcag atcttcggcc cgagcgcgct gcagaacgga
     1381 acgttccgtc agaagaaccg ccttccggac ggcacggtcg tggagacgca gggcaccttc
     1441 gaggcggagt gggaccgcca gcccgacggc tcgtggctcg tcgcccggat gacgatcaac
     1501 ccgccgccct ttacgccgcc ggacgcgggc tcggtgccgt gagcgctgga cgcgcgaagg
     1561 tctacgacca cgcgtacttt cagacctggt atcgcgaccc gaagaaggcg atcgtgcggc
     1621 gcgcggacct cgagcggaag gtcgcgatgg cgctcggcgc cgccgagcac ctcctcggac
     1681 gcaccgtcga ctccgtgctc gacgtcggct gcggcgaagg atcgtggcag cccatcctcc
     1741 gcaggctccg gccccgcctg aagtacctcg gcctcgacac gagcgaatac gcgatccgcc
     1801 gctacggccg ctcgcggaac ctgcgcgccg gctcgtgggg ccagctctcc gagctgcgat
     1861 tcggcgggcc gttcgacctc gtcgtctgct ccgatgcgct ccattacgtg cccacgaagg
     1921 aactcctgcg ggggctcggc ggactgcccg cgctcgtcgg cggcgccgcg tacctcgact
     1981 tcctcacgtc ggacgacgac ctcgagaccg ggctcgaggg cgacctcgtg ggattccgga
     2041 agcgcacggc cgcgtggtac cggaagaagc tgcgcgcgac gggcctcgtc gagtgcggcc
     2101 tcgggatctg ggcggcggcg gagctcgccc cggcgctcac ggcgatggag cgcgggccaa
     2161 agcagccccg ttgacttacg cctgacttgc gtctaacgtt ccacgtccca tggacgggcg
     2221 cggggacggg aagcacggcg ggccgccggg gcgcgacatg ctcggcccgc tcgtgggcgg
     2281 cgaccgcgcg aaggcgtacg tcgggcccgg cgcgcacaag gaaaagctgc gcaagcgcga
     2341 ctacgcgcgc cccgcggcgc gcgtgcggc
//
LOCUS       DAOMFY010000422         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_21565_length_2369_cov_6.245462, whole genome shotgun sequence.
ACCESSION   DAOMFY010000422 DAOMFY010000000
VERSION     DAOMFY010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21565_length_2369_cov_6.245462"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1106
                     /gene="lpxD"
                     /locus_tag="PLB01_19510"
     CDS             75..1106
                     /gene="lpxD"
                     /locus_tag="PLB01_19510"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQQ79542.1"
                     /translation="MAAFTVAEVAAACGGRVVGDGAARVSGVRALENAGPESLSFAID
                     AKALKRASASRAGAFLARSASDLPGRTVVEVADPQQALIAVLRLFHPLRTARPGIHPT
                     AVVDPGATVDPSAEIGPYAVIGAGTVVGAGVVVEAHAVVGAGCRVGAGSWLHPHVVLY
                     DRVTLGERVEVHGGAVLGADGFGYATTARGLVKIPQVGTVSVGDDVEIGANTCIDRAA
                     LETTTVGAGTKVDNLVQIGHNVIVGRHDVICAQVGIAGSAVLEDGVVLGGQVGVAGHL
                     TVGAGAQVQAQSGIGADVPPKQALHGSPAFGYRDYQRSWIELRRLPETARLVRRLADK
                     AGLLKGGES"
     gene            1103..1579
                     /gene="fabZ"
                     /locus_tag="PLB01_19515"
     CDS             1103..1579
                     /gene="fabZ"
                     /locus_tag="PLB01_19515"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HQQ79543.1"
                     /translation="MSEFVSKADILEIQRLLPHRYPFLLVDRILDISPGPVAVGSKVV
                     GLKNVSVNEPFFQGHFPGNPVMPGVLIIECMAQVAGCLFFSKEKDPSAKLMFLSGVDK
                     ARFRRPILPGDQIIVNAEIVSARLGLVKIKAEARVDGALCAEAEIMSSLVNRPGAA"
     gene            1579..2358
                     /gene="lpxA"
                     /locus_tag="PLB01_19520"
     CDS             1579..2358
                     /gene="lpxA"
                     /locus_tag="PLB01_19520"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HQQ79544.1"
                     /translation="MPIHPTAVVDPTASVHPTADVGPYAIIGPNVTIGARTTVGAHAV
                     IEGPTTIGEDNRICPQAALGGPPQDLKYKGEPTTLVIGNRNMFREFCTVHRGTVTGHK
                     TTVIGDDNLFMAYAHVAHDCTVGNRTVFANGASLAGHCVVEDDVILGGYAAVRQFLRV
                     GRHAFVGAFSGINRDILPFVWSSSDRDVKAYKVNAVGLSRKGFSKERVEALQKAYRIL
                     HKHRHDKTALLAGLEPLAAASDDVRQLRDFIVSSKEGVHGA"
BASE COUNT          342 a          876 c          810 g          341 t
ORIGIN      
        1 ggccgccgcc gccgcgcccg caccggccgc tgccaagccg gccgcaccgg ccgcgcccaa
       61 gaagtaaccg ccgcgtggct gcgttcaccg tcgccgaggt ggccgcggcc tgcggcggcc
      121 gagtcgttgg cgatggcgca gctcgggtga gcggcgtccg cgccctcgag aacgcgggcc
      181 ccgagtcgct gtccttcgcg atcgacgcca aggcgctcaa acgcgcctcg gcgtcccgcg
      241 cgggcgcctt tctcgcgcgc tccgcgtccg acctgcccgg gcggacggtc gtcgaagtgg
      301 ccgaccccca gcaggcgttg atcgccgtcc tgcggctctt ccacccgctc cggaccgcac
      361 ggcctggaat ccacccgacc gcggtcgtgg atcccggcgc gaccgtggac ccgtcggccg
      421 agatcggacc ctacgccgtg atcggcgccg ggacggtcgt cggggcggga gtcgtcgtcg
      481 aggcgcacgc cgtcgtcggc gcgggctgcc gcgtcggcgc cggctcgtgg cttcaccccc
      541 acgtcgtcct ctacgaccgc gtcacgctcg gcgagcgcgt cgaggttcac ggcggcgccg
      601 tgctcggggc cgacggcttc ggctacgcga cgaccgcccg cggcctcgtg aagatcccgc
      661 aggtcgggac ggtgtcggtc ggagacgacg tcgagatcgg cgcgaacacg tgcatcgacc
      721 gggccgcgct cgagacgacg acggtgggcg ccggcacgaa agtcgacaac ctcgtccaga
      781 tcggccacaa cgtgatcgtc ggccgccacg acgtcatctg cgcccaggtc ggcatcgcgg
      841 gctcggccgt cctcgaggac ggcgtcgtcc tcggcggcca ggtcggcgtc gcggggcacc
      901 tcacggtcgg cgcgggcgcc caggtgcagg cccagagcgg catcggcgcc gacgtccctc
      961 cgaagcaggc gctccacggc tcgccggcgt tcggttatcg cgactaccaa agatcctgga
     1021 tcgagctccg gcggctgccg gagaccgccc gcctcgtgcg ccgcctcgcg gacaaggccg
     1081 ggctgctcaa gggaggggag tcgtgagcga gttcgtttcg aaggccgaca tcctcgaaat
     1141 ccagcggctc ctgcctcacc gctacccgtt cctgctcgtc gaccgcatcc tcgacatctc
     1201 gccgggcccc gtcgcggtgg gctcgaaggt cgtggggctc aagaacgtgt ccgtgaacga
     1261 gccgttcttc caggggcatt tccccgggaa cccggtcatg cccggcgtcc tgatcatcga
     1321 gtgcatggcg caggtcgccg gctgcctgtt cttctcgaag gagaaggacc cgtccgcgaa
     1381 gctgatgttc ctgtccggcg tcgacaaggc ccgcttccgg cggcccatcc tcccggggga
     1441 ccagatcatc gtcaacgccg agatcgtgag cgcgcggctc ggcctcgtga agatcaaggc
     1501 agaggcccgc gtggacggcg ccctctgtgc ggaggccgag atcatgtcga gcctcgtgaa
     1561 ccgtccggga gccgcctgat gccgatccac ccgaccgccg tcgtggaccc gacggcttcc
     1621 gtccatccca ccgccgacgt cggcccgtac gcgatcatcg gcccgaacgt cacgatcggc
     1681 gcccgcacga cggtgggcgc ccacgccgtc atcgaaggcc cgacgacgat cggagaggac
     1741 aaccggatct gcccgcaggc cgccctcgga gggccgcccc aggacctcaa gtacaagggc
     1801 gagccgacga cgctcgtcat cgggaaccgg aacatgttcc gggagttctg cacggtccat
     1861 cgcggaacgg tgacggggca caagacgacg gtcatcggcg acgacaacct cttcatggcc
     1921 tacgcgcacg tcgcgcacga ctgcacggtc gggaaccgga ccgtgttcgc caacggcgct
     1981 tcgctcgcgg gccactgcgt cgtcgaggac gacgtcatcc tcggcgggta cgcggccgtt
     2041 cgccagttcc tcagggtcgg gcgccacgcg ttcgtcggcg ccttctccgg gatcaaccgc
     2101 gacatcctgc cgttcgtgtg gagctcctcg gaccgcgacg tgaaggccta caaggtgaac
     2161 gccgtgggcc tctcacggaa gggcttctcg aaggagcgcg tcgaggccct ccagaaggcc
     2221 taccgcatcc tccacaagca ccgccacgac aagaccgcgc tccttgccgg cctcgagccg
     2281 ctcgccgcgg cttcggacga cgtccggcag ctccgggact tcatcgtttc ctcgaaggaa
     2341 ggcgttcacg gggcgtgatc cctgcgcgg
//
LOCUS       DAOMFY010000423         2364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_21648_length_2364_cov_6.429623, whole genome shotgun sequence.
ACCESSION   DAOMFY010000423 DAOMFY010000000
VERSION     DAOMFY010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21648_length_2364_cov_6.429623"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PLB01_19525"
     CDS             <1..1173
                     /locus_tag="PLB01_19525"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQQ79545.1"
                     /translation="PAPRPRPRMTAGAGAPDARRWPRSFFVLVPLGAAVIALLAVELA
                     LRLASPVPYAPEHNMVFDPDPDTGYRLRPGGVARFGNGARGVASGQGFRSREVGPKGP
                     GTFRILAVGDSFTEGAGVEAEEAWPQVVEGLLARSGPRRVEVVNAGVGGWDPFQYAQF
                     FESRGLALEPDLVVVGFFVGNDAYSRFLTVESLWTAVGGRRVTREAARRPSIRIAVFL
                     QEHFHLARALRGRPPGFAPRRSCEDFSPWLLEAETDRLENHLKRSPEREARARPNVDQ
                     VLRIRLFAQAKGVPVVVALFAEESQVHAALRARLLAGRDAGAYDFEMPQAMLREMFAA
                     EGVPVVDLLPDFRRDPRCLYMNDTHWNPAGHVLAARVLADALLPLIAPARGSAPAP"
     gene            1170..1568
                     /locus_tag="PLB01_19530"
     CDS             1170..1568
                     /locus_tag="PLB01_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79546.1"
                     /translation="MTSALRRAAASLLAAAVLLVLALRAWEAVAPLPVSLSVMGKFWR
                     AGTPARLLNSRLFRRDPNFGVALLGRDRSLPLGADVVLTVPPALPDAAAEEMRRKAAF
                     VLAPRRVTLARGETGREGFALAAAPAEGAP"
     gene            1565..>2364
                     /locus_tag="PLB01_19535"
     CDS             1565..>2364
                     /locus_tag="PLB01_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79547.1"
                     /translation="MSRSASRVVGAAALLLLVLGVFTAGVYAMREPFALNDYTAIWGL
                     KARALHRSGSLASLFRVDPGGAFSHPEYPPLWPLLLTVATRARLPYDDLVATPMWPLL
                     ALAASLLAVRAALKGNVAPPFALLAGAAVSLLPYWRTYPGYAEGPLLVFVLAAAGEAG
                     SAGTDAAATVRLSIFLTLAAWTKPEGLVAALAAAAVLALTRRFRAALLVGLSALLFAA
                     LPWALVVRRLAPQPLPTAFALPAFSLGKAAAALAALAAEAAPAAGLALA"
BASE COUNT          213 a          936 c          836 g          379 t
ORIGIN      
        1 cctgcgccgc gccctcggcc gcggatgacc gccggcgccg gcgcaccgga cgcgcggcgc
       61 tggccgcgaa gcttcttcgt tctcgttccg ctgggcgccg cggtgattgc gcttctcgcc
      121 gtcgagctcg cgctgcggct cgcgagtccc gtcccgtacg cgcccgagca caacatggtc
      181 ttcgaccccg atccggacac cggttaccgg ctgaggccgg gcggagttgc ccgcttcgga
      241 aacggcgcgc ggggcgtcgc gagcgggcag gggttccgca gccgcgaggt cggcccgaag
      301 ggccccggca cgttccgcat cctggccgtc ggcgactcct tcaccgaggg cgcgggcgtg
      361 gaggcggagg aggcctggcc gcaggtcgtg gagggccttc tcgcccgctc cgggccgcgg
      421 cgcgtcgagg tcgtgaacgc cggcgtcggc ggctgggacc cgttccagta cgcgcagttc
      481 ttcgagagcc gcggcctcgc gctcgagccc gacctcgtcg tcgtggggtt cttcgtgggg
      541 aacgacgcct acagccgctt cctgacggtc gagtccctgt ggacggccgt cggggggcgc
      601 cgcgtcacgc gggaggcggc gcggcggcct tccatccgca tcgccgtgtt cctgcaggag
      661 cacttccatc tcgcgcgggc gctccggggc cgcccgcccg gcttcgcgcc gcgccgctcg
      721 tgcgaggact tctccccgtg gctcctcgag gccgagaccg atcggctcga gaaccacctg
      781 aagcgctcgc ccgagcgcga agcgcgcgca cggccgaacg tggaccaggt cctgcggatc
      841 cggctcttcg ctcaggcgaa gggcgttccc gtcgtcgtgg cgctcttcgc cgaggagagc
      901 caggtccacg cggccctccg ggcgcggctc ctcgccgggc gggacgcggg cgcgtacgat
      961 ttcgagatgc cccaggcgat gctgcgcgag atgttcgccg cggaaggcgt gcccgtcgtc
     1021 gacctgctgc cggatttccg gcgcgatccg cgctgcctct acatgaacga cacgcactgg
     1081 aatcccgcgg ggcacgtgct cgcggcccgc gtcctcgcgg acgccctgct cccgcttatc
     1141 gctcccgcga gaggctccgc gccagcgcca tgacctccgc gctgcggaga gcggccgcgt
     1201 ctctcctcgc cgcggccgtg ctcctcgtcc tggcgctgcg ggcgtgggag gccgtcgcgc
     1261 cgcttccggt ctccctgtcc gtgatgggga aattctggcg cgcgggcacc ccggcgcggc
     1321 tcctgaactc gcgcctcttc cggcgcgatc cgaacttcgg cgttgcgctc ctcgggcgcg
     1381 accggtccct gccgctcggc gcggacgtcg tcctcaccgt gccgccggcg ctgcctgatg
     1441 ccgccgccga ggagatgcgc cgcaaggcgg ccttcgtcct cgcgccgcgg cgcgtgacgc
     1501 tcgcccgcgg ggagacgggc cgcgaggggt tcgcactcgc cgcggctccc gccgagggcg
     1561 cgccgtgagc cgttccgcgt cccgcgtcgt cggtgcggcg gcgctccttc tcctcgtcct
     1621 cggcgtcttc acggcggggg tctacgcgat gcgcgaaccc ttcgccctca acgactacac
     1681 ggcgatctgg ggtctcaagg cgcgcgctct ccaccgttcg ggctccctcg cgtccctctt
     1741 ccgcgtggat cccggcggcg cgttttccca tcccgagtac ccgccgctct ggccgctcct
     1801 cctcacggtg gcgacgcggg cgcgcctccc gtacgacgac ctcgtcgcga cgccgatgtg
     1861 gccgctcctc gcgctcgccg cgtcgctcct cgcggtgcgc gcggcgttga agggaaacgt
     1921 cgcgccgccc ttcgcgctcc tcgcgggtgc cgccgtgtcg ctgctcccgt actggcggac
     1981 gtacccgggc tacgcggagg ggcccctcct cgtcttcgtc ctcgcggctg ccggcgaggc
     2041 cgggagcgcc ggcacggacg cggctgcgac cgtacgcctc tcgatcttcc tcacgctcgc
     2101 ggcgtggacg aagcccgagg gcctcgtcgc ggcgctggcc gccgccgccg tcctcgccct
     2161 tacgcgacgc tttcgcgcgg cgctcctcgt cgggctctcc gcgcttctct tcgccgctct
     2221 tccctgggcg ctcgtcgtcc ggcggcttgc gcctcagccc ctgccgacgg cattcgcgct
     2281 tccggcgttc tcgctcggga aggccgctgc ggccctcgcc gcgctcgccg cggaagcggc
     2341 tccggccgcg ggcctcgccc tcgc
//
LOCUS       DAOMFY010000424         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_22991_length_2290_cov_9.442058, whole genome shotgun sequence.
ACCESSION   DAOMFY010000424 DAOMFY010000000
VERSION     DAOMFY010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22991_length_2290_cov_9.442058"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..328)
                     /locus_tag="PLB01_19540"
     tRNA            complement(252..328)
                     /locus_tag="PLB01_19540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Met,seq:cat)
     gene            384..1409
                     /locus_tag="PLB01_19545"
     CDS             384..1409
                     /locus_tag="PLB01_19545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HQQ79548.1"
                     /translation="MPVRILFLSDTHLGLDLPARPRIERPRRGDDLFESFERALAPAT
                     TGEADVVVHGGDLFYRSRIPAWLAERVFARLAALADSGVDVFWVPGNHERSGVPRGLL
                     LTHPGIRVFDRPRTFVVERGGLTISLSGFPYAPRVAADFAALLEATGHRETAADVKLL
                     CLHQAVEGATVGPSDFVFRSGDDVLKGRDVPSGFAAVLSGHIHRSQALVADLAGRPLA
                     APVLYAGSTERTSFAERHEPKGFLVLEVAAGGGGGRATWEFRDLAARPMVDVALDPRA
                     DDLEARLKAALANLDPRSIVRLSLTVDPPVHVLPFLRAESLRAAAPRSMSVSVAWRRA
                     PGGFARR"
     gene            1448..1909
                     /locus_tag="PLB01_19550"
     CDS             1448..1909
                     /locus_tag="PLB01_19550"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1598..1600,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQQ79549.1"
                     /translation="MALFSESTLKPGDPAPDFTLKDQTGKDVRLSKLRGKRVVLYFFP
                     KADTAGUTVEAQAFRDEGPKLPNDVVVLGVSKDTVEAQKKFAAKNKLTFRLLADEDSE
                     VIALYGVSLAFGVAKRRSFLIDSKGKIAKVYESVSPAKHAGEVARDLEGVP"
     gene            complement(1906..>2290)
                     /locus_tag="PLB01_19555"
     CDS             complement(1906..>2290)
                     /locus_tag="PLB01_19555"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQQ79550.1"
                     /translation="RTDHGPEAPAARPDARHVRPASVIVVSALLSALVFGIFLVSDRG
                     LLQVRKQRVQLAKAQEEVAQLEADTKRLQAEVAALKSDPHALEKVAREELNLVRPGEI
                     VLVLPDGWEQRVKPAAPPAPSPVSR"
BASE COUNT          347 a          822 c          770 g          351 t
ORIGIN      
        1 tgcctcaagc cgtcgctatg gccatctcgg gccacaagac gccgtcgatg ttcctccgct
       61 acaacatcac cgacgagcgg gacaagcgac agggacttct cgcgacccag gcgtacgtcg
      121 cttcccagcc gaaggaggag gcgaaggtgg tccagatcag cgccgccccg gctccggcga
      181 aaacccggac aaaacccgga caatcaaaga aaaaggggtt agctcgaatc gagctaaccc
      241 ctttagattc atggttgcgg gggcaagatt tgaacttgcg acctttgggt tatgagccca
      301 acgagctacc aggctgctcc accccgcgtc agggaggggg aacatagccg cgggagggtc
      361 gcgagtcaag ttacgctttc ctcgtgccgg ttcgcatcct cttcctctcc gacacgcatc
      421 tcgggctcga ccttcccgcg aggccgagga tcgagcggcc gcggcgcggc gacgacctct
      481 tcgagagctt cgagagggca ctcgcacccg cgacgaccgg ggaggccgac gtcgtcgtcc
      541 acggcggcga cctcttctac cggagccgca tccccgcctg gctcgccgaa cgggtcttcg
      601 cgcggctcgc ggcgctcgcg gactcgggtg tcgacgtctt ctgggtgccc gggaaccacg
      661 agcgctccgg cgtgccccgc ggcctcctcc tgacgcatcc cgggatccgc gtcttcgacc
      721 gtccgcggac gttcgtcgtg gagcgcggcg gcctcacgat ctccctctcg ggcttcccgt
      781 acgcgccgcg cgtcgcggcg gacttcgccg cgctcctcga ggcgaccggt caccgcgaga
      841 ccgccgccga cgtgaagctc ctgtgcctcc accaggccgt cgagggcgcc acggtggggc
      901 cttccgactt cgtcttccgg agcggggacg acgtgctcaa gggccgggac gtcccctccg
      961 ggttcgcggc cgtcctctcg ggccacatcc accggtcaca ggccctcgtg gcggatctcg
     1021 ccgggcggcc tctcgcggcg cccgtcctct acgcaggctc cacggagcgg acctccttcg
     1081 cggagaggca cgagccgaag ggctttctcg tgctcgaggt cgctgccggc ggcggaggcg
     1141 gacgcgcgac gtgggagttc cgtgacctcg cggcccgccc gatggtcgac gtcgctctcg
     1201 acccgcgggc cgacgacctc gaggcgcgcc tcaaggccgc cctcgcgaac ctcgatccgc
     1261 ggagcatcgt gaggctgtct ctcacggtcg atcccccggt gcacgtcctt ccgttcctcc
     1321 gcgccgagtc cctccgcgcc gcggccccgc gctcgatgag cgtctccgtc gcgtggcgga
     1381 gggcgccggg cggcttcgcg cgccgctgag gccccacatg gccgagaatg gccggacgga
     1441 ggccccgatg gctctcttct ccgaatcgac gctcaagccc ggcgatcccg cgccggattt
     1501 caccctgaag gaccagaccg gaaaggacgt gcggctctcg aagctgcgcg gaaagcgcgt
     1561 cgtcctctac ttctttccga aggccgacac cgccggttga accgtggaag cccaggcctt
     1621 ccgtgacgaa ggcccgaagc tcccgaacga cgtcgtcgtc ctcggcgtct cgaaagacac
     1681 cgtggaggcg cagaagaagt tcgcggcgaa gaacaagctg acgttccgcc tcctcgcgga
     1741 cgaggacagc gaggtcatcg cgctctacgg cgtcagcctc gcgttcggcg tcgcgaagcg
     1801 gagatccttc ctgatcgact ccaagggaaa gatcgcgaag gtctacgagt ccgtctcgcc
     1861 ggcgaagcac gcgggcgagg tcgcgaggga tctcgaggga gttccctagc gcgacacggg
     1921 cgacggagcg ggaggcgccg cgggcttcac gcgctgttcc cacccatccg gcaggacgag
     1981 aacgatctcg ccgggccgga cgaggttgag ctcctcgcgc gcgaccttct cgagtgcgtg
     2041 cgggtcgctc ttgagcgccg cgacctcggc ctggagccgc ttcgtgtcgg cctcgagctg
     2101 ggcgacctcc tcctgggctt tcgcgagctg gacgcgctgc ttcctcacct gcaggaggcc
     2161 gcgatcacta acgaggaaga tgccgaacac gagggccgag agcagcgccg acacgacgat
     2221 gacggacgcc gggcgcacgt ggcgcgcgtc gggccgcgcc gcgggcgcct cggggccgtg
     2281 gtccgtcctc
//
LOCUS       DAOMFY010000425         2282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_23136_length_2282_cov_6.028738, whole genome shotgun sequence.
ACCESSION   DAOMFY010000425 DAOMFY010000000
VERSION     DAOMFY010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23136_length_2282_cov_6.028738"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PLB01_19560"
     CDS             <1..1006
                     /locus_tag="PLB01_19560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ79551.1"
                     /translation="LAEALLDARDAGLLAGKIAGDDGSRGAALFAMPAEGRAASALLD
                     AGRTRAFLKIQDGCRRRCAFCIVPKLRGAERSAALRDVADAVRALGAAGVPEVVLTGI
                     HLAAFGGKDGGLLALLDDLERDPPACRVRLSSLEPMEAGVELAARVAVSRVVVPHLHL
                     PLQSGSASVLRRMRRGIVPPRYRDLARAASRANPRLHLATDLIAGFPGETDAEFEETL
                     RFVEELPFASIHVFPFSPRAGTDAAAWHRVAGVPAAAITERTRALRRLGERKAKDFAA
                     RAEGTVADLVVLRGGRGLTDHYLDVGLRLPAADAAPGRRLAVRLAASAADGSLLAHPC
                     "
     gene            1083..1823
                     /locus_tag="PLB01_19565"
     CDS             1083..1823
                     /locus_tag="PLB01_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQQ79552.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGRLFTKLIKEITMAAKVGGGVVD
                     SNPRLRSAVLAAKKASMPSDNIDKAIKRGTGELEGVTYEELTYEGYGPGGAAILIEVM
                     TDNKNRTASEVRHLLTKYGGNMAAAGAVAFQFTKKGVIAVEKSAVKEDVLMELALESG
                     AEDFKTEDPDVFEILTDPASFDAVKAAVEAKNIAIQGAEIQMVPQSTIDVGDKGPALL
                     KLLEMVEDNDDVQKVWSNGDIDEKYLQE"
     gene            1825..>2282
                     /locus_tag="PLB01_19570"
     CDS             1825..>2282
                     /locus_tag="PLB01_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQQ79553.1"
                     /translation="MKILGVDPGSRAAGWAVVDFAAAPRLVAAGVIRPPAALPFAERL
                     AHLHDALGEIAVREAPQAAVVEKVFAGVNPQSLIALGEARGVLLLALARAGLTPAEIT
                     PAEIKRAVAGSGGAEKEQVRRMVAALLGRRLALDAADAAAAALAHGFVVAR"
BASE COUNT          346 a          849 c          786 g          301 t
ORIGIN      
        1 cctcgccgag gcgctcctcg acgcccgcga cgcgggcctc ctcgcgggca agatcgcggg
       61 cgacgacgga agccgcggcg ccgcgctctt cgcgatgccc gccgagggac gggccgcctc
      121 cgcgctcctc gacgccgggc gcacgcgcgc gttcctgaag atccaggacg gctgccgccg
      181 gcgctgcgcg ttctgcatcg tcccgaagct gcgcggggcc gagcgcagcg ccgcgctccg
      241 ggacgtcgcg gacgccgtgc gggcgctcgg cgccgccggc gttcccgagg tcgtcctgac
      301 gggcattcat ctcgcggcgt tcggcggaaa agacggcggc ctgctcgccc tcctcgacga
      361 cctcgaacgc gacccgccgg cgtgccgtgt ccgcctgtcg tcgctcgagc cgatggaggc
      421 cggcgtcgag ctcgccgcgc gcgtcgccgt gtctcgtgtc gtcgtcccgc acctccacct
      481 tccgctccag tcgggctccg cctcggtcct caggcggatg cgccggggaa tcgttccgcc
      541 gcgctatcgc gacctcgccc gcgcggcctc ccgtgcaaac ccgcgcctcc acctcgcgac
      601 cgacctcatc gccggtttcc ccggcgagac ggacgcggag ttcgaggaaa cgctgcgctt
      661 cgtcgaggaa cttcccttcg catcgatcca cgttttcccg ttttcccctc gcgccgggac
      721 ggacgcagcc gcgtggcacc gcgtggccgg cgtgcccgcc gccgcgatca ccgaacgcac
      781 ccgcgccctc cggcggctcg gggaacgaaa ggcgaaagac ttcgcggccc gcgcagaggg
      841 aaccgtcgcc gatctcgtcg tcctgcgcgg cggacggggc ctgaccgacc actatctcga
      901 cgtcggactg cgcctcccgg cagccgacgc ggcgcccggc cgccggctcg cggtccgact
      961 ggcggcatcg gcggcagacg gttcgctgct cgcacatccc tgctagactc tgcggccccg
     1021 aagagcccgt cccgccgcgg tttcgcgcgg agaaggggct cgatcacgcc tgaggtgagg
     1081 ccatgtccgg acacagcaag tggagcacga tcaagcacaa gaagggcgcc gccgacgcca
     1141 agcgcgggcg cctcttcacg aagctgatca aggaaatcac gatggccgcc aaggtcggag
     1201 gcggtgtcgt ggactccaac ccgcgcctga ggtcggccgt ccttgccgcc aagaaggcgt
     1261 cgatgccgtc ggacaacatc gacaaggcga tcaagcgcgg cacgggcgag ctcgagggcg
     1321 tcacctacga ggagctcacg tacgagggat acgggcccgg cggcgccgcg atcctcatcg
     1381 aggtcatgac ggacaacaag aaccggacgg cttccgaggt ccgccatctc ctgacgaagt
     1441 acggcggcaa catggcggcc gccggcgccg tggcgttcca gttcacgaaa aagggcgtca
     1501 tcgccgtcga gaagagcgcc gtcaaggaag acgtcctcat ggagctcgcg ctcgagtcgg
     1561 gcgccgagga cttcaagact gaagacccgg acgtgttcga gatcctcacg gaccccgcgt
     1621 ccttcgacgc cgtgaaggcc gccgtcgagg cgaagaacat cgcgatccag ggcgccgaga
     1681 tccagatggt cccgcagagc acgatcgacg tcggcgacaa gggacccgcg ctcctcaagc
     1741 tcctcgagat ggtcgaggac aacgacgacg tgcagaaggt ctggtcgaac ggcgacatcg
     1801 acgaaaaata cctgcaggag taggttgaag attctcggcg tcgacccggg gagtcgagcg
     1861 gccggatggg ccgtggtgga cttcgccgcg gcgccgcggc tcgtcgcggc cggcgtcatc
     1921 cggccgccgg cagcgttgcc cttcgccgag cgcctcgccc acctccacga cgcactcggc
     1981 gagatcgccg tgcgcgaggc cccccaggcc gccgtcgtgg agaaggtctt cgcgggcgtc
     2041 aacccgcaga gcctcatcgc cctcggcgag gcccgcggcg tcctcctcct cgcgctcgcc
     2101 cgggccggcc tcacgccggc cgagatcacg cccgcggaga tcaaacgcgc cgtcgccgga
     2161 tcgggcggcg ccgagaagga acaggtccgc cggatggtgg cggcgctcct cgggaggcgc
     2221 ctcgcgctcg acgcggcgga cgccgccgca gcggcactcg cgcacggctt cgtcgtcgcc
     2281 cg
//
LOCUS       DAOMFY010000426         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_23274_length_2274_cov_6.441640, whole genome shotgun sequence.
ACCESSION   DAOMFY010000426 DAOMFY010000000
VERSION     DAOMFY010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23274_length_2274_cov_6.441640"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..799)
                     /locus_tag="PLB01_19575"
     CDS             complement(110..799)
                     /locus_tag="PLB01_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79554.1"
                     /translation="MRRQTLPPAAGLVIAGALLAGSALAGDVRVTVSVVTPEKKTVPG
                     AGAVVWVVNAAAAKQAAPSAKPRIASKSKRFDPHVAVVPAGGTVQFPNLDGIYHNVFS
                     LSEKARFDLGLYRNGASRAMSFENPGVVKIYCNIHPQMAAVLLVIEGGIWAQAGEDGT
                     AVLAHVPAGRTTVRAWDEKGGEFTAVVDVPETGTVPLAVSFDGSGWRETAHKNKYGKD
                     YPPPDDDGNRY"
     gene            complement(814..1995)
                     /locus_tag="PLB01_19580"
     CDS             complement(814..1995)
                     /locus_tag="PLB01_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQQ79555.1"
                     /translation="MAPKLPVYLDYHATTPVDPRVFEAMRPYFTEHFGNAASRSHVFG
                     MRAASAVEKAREEVADLVGANAKEIVFTSGATEADNLAVLGAARARRAMGAHVVVSSI
                     EHRAVLDPCARLEAEGFRVTRVAPDRLGRTGAAAVAWALSAETVLVTVMAANNEIGTL
                     NPVAEIAGLCKERGILFHTDAVQAAGKVPLDVHAAGIDLLSLSAHKLCGPKGVGALFV
                     RASNPRVTLDPILFGGGHERGMRPGTLDVPGIVGFGAAAAIAKAEGPAEAVRVSALRD
                     RLWERLRAAVPGASRNGDPAASLPGNLNVRFPGAPSDTVMMETRDLAVSAGSACTSAS
                     VEPSHVLRGIGLTKEEAHESIRFGLGRFTSEEDVDWAADAVASAVRRVRAARGTTGPL
                     V"
     gene            2051..>2274
                     /locus_tag="PLB01_19585"
     CDS             2051..>2274
                     /locus_tag="PLB01_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79556.1"
                     /translation="MRHVLVATSERHDLLDALRALAPPSTTFLGEQGLENVLERLGRS
                     ARLDAVVTDDPEMVTEIFHEIPGAIPVYLAR"
BASE COUNT          319 a          830 c          819 g          306 t
ORIGIN      
        1 cggcggggcc acgaacgtcg gctccgccgg cgcacgggtg acgcgcgtcg cctcaccgac
       61 gtcgagcgtc acgcgcgtcg cctctgcgga cgcggacctg cgggtgtcgt cagtaccggt
      121 tgccatcgtc gtcgggagga gggtagtcct ttccgtactt gttcttgtgc gcggtctcgc
      181 gccatccgga gccgtcgaag gaaacggcga gcggcaccgt cccggtctcc ggcacgtcga
      241 cgacggccgt gaactcgccg cctttctcgt cccacgcgcg gaccgtcgtc cgtcccgcgg
      301 gcacgtgcgc gaggaccgcc gtcccgtcct ctccggcctg cgcccagatc ccgccctcga
      361 tgacgagcag gacggccgcc atctgggggt ggatgttgca gtagatcttc acgacccccg
      421 ggttctcgaa cgacatggcc cgcgaggcgc cgttgcggta gaggccgagg tcgaaccgcg
      481 ccttctcgga gaggctgaag acgttgtggt agatcccgtc gaggttcggg aactgcacgg
      541 tgccgccggc gggcacgacg gcgacgtgcg ggtcgaagcg cttgctcttc gacgcgatcc
      601 tcggcttcgc ggacggcgcg gcctgcttcg ccgcggcggc gttgacgacc cagacgacgg
      661 cgcccgctcc cggcacggtc ttcttctcgg gcgtcacgac cgaaaccgtg acgcggacgt
      721 cgcccgcgag ggccgatccg gcgaggaggg cgcccgcgat cacgaggccc gcggcgggag
      781 gaagcgtctg gcgccgcacg ggtgagatcg attctagacg agggggcccg tcgtcccgcg
      841 ggcggcgcgc acgcgccgaa cggccgacgc gaccgcgtcg gcggcccagt cgacgtcctc
      901 ctccgacgtg aaccgcccga ggccgaagcg aatcgactcg tgcgcctcct ctttcgtcag
      961 cccgatcccg cggaggacgt ggctcggctc gacggacgcc gacgtgcagg ccgagcccgc
     1021 ggacaccgcg aggtcgcgcg tctccatcat cacggtgtcg gacggcgcac ccgggaagcg
     1081 aacgttcagg ttgcccggca gcgaggccgc gggatcgccg ttgcgcgaag cgccggggac
     1141 ggcggcgcgg agccgctccc agagccggtc gcgcagcgcc gagacccgca ccgcctcggc
     1201 gggcccctcg gccttcgcga tcgccgccgc cgcgccgaag cccacgatac cggggacgtc
     1261 gagcgtcccg ggcctcatcc cgcgctcgtg cccgccgccg aagagaatcg ggtcgagcgt
     1321 gacgcgcgga ttcgacgcgc gcacgaacag tgcgccgaca cccttcggac cgcagagctt
     1381 gtgggccgag agcgagagaa ggtcgattcc agcggcgtga acgtcgagcg ggaccttccc
     1441 cgcggcctgg acggcgtccg tgtggaacag gatgccgcgc tccttgcaga ggcccgcgat
     1501 ctccgcgacg ggattgagcg tcccgatctc gttgttcgcc gccatgacgg tcacgaggac
     1561 cgtctcggcg gagagcgccc acgcgacggc ggcggcgccc gtgcggccga gccgatcggg
     1621 ggcgacgcgc gtgacgcgga agccctcggc ctcgaggcgc gcgcaggggt cgaggacggc
     1681 gcgatgctcg atcgacgaga ccacgacgtg cgcgcccatc gcgcgccgcg ccctcgcggc
     1741 gccgaggact gcgaggttgt cggcctcggt cgcgccggac gtgaagacga tctccttcgc
     1801 gttggcgccg acgaggtcgg cgacttcctc ccgcgccttc tcgacggcgg aggcggcgcg
     1861 catcccgaag acgtggctcc gggaggcggc gttcccgaag tgctccgtga agtacgggcg
     1921 catcgcctcg aagacgcgcg ggtcgaccgg cgtcgtcgcg tgataatcga ggtacacggg
     1981 aagcttcggc gccacgcccc atcgtcggcc cgctgcgaac cgattgcaag gcggagagaa
     2041 gaccgacccc atgcgccacg tcctcgtcgc gaccagcgag cgccacgacc tcctcgacgc
     2101 gctgcgcgcg ctcgcgccgc cgtcgacgac gttcctcggc gagcaggggc tcgagaacgt
     2161 cctcgagcgc ctcggccgct ccgcccgtct cgacgccgtc gtgaccgacg acccggaaat
     2221 ggtcacggag atcttccacg agatccccgg cgcgatcccc gtctacctcg cgcg
//
LOCUS       DAOMFY010000427         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_23710_length_2250_cov_6.170387, whole genome shotgun sequence.
ACCESSION   DAOMFY010000427 DAOMFY010000000
VERSION     DAOMFY010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23710_length_2250_cov_6.170387"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..419)
                     /locus_tag="PLB01_19590"
     CDS             complement(63..419)
                     /locus_tag="PLB01_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQQ79557.1"
                     /translation="MSPTFADPSCLFCRIGRRETPARIAFENDHVLAFHDVAPKSPTH
                     VLVIPKVHVAALSGTTAAHAETLGACLAAVAEIARTLGLSSYRVVANDGPDAGQSVFH
                     LHFHLMGGRSFAWPPG"
     gene            complement(427..1713)
                     /gene="murA"
                     /locus_tag="PLB01_19595"
     CDS             complement(427..1713)
                     /gene="murA"
                     /locus_tag="PLB01_19595"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQQ79558.1"
                     /translation="MDAFLLKGPVRLEGTVSASGAKNAALPEMAAALLADAPVVLDGV
                     PDLADVRTMERLVAGMGVSLGRPAPGRVVLDASRVTSTEAPYDLVRTMRASILVLGPL
                     VARFGEARVSLPGGCAIGVRPVDLHLMALEAMGARVAVEKGYIHAHVSRVSRARGRLT
                     GATVRFPTPTVTGTENVLFAAALARGTTVIENAAREPEVEDTARLLVRMGANVSGAGN
                     STITVEGVERLRGTGEAPHAVVPDRIEAGTYLAAGAITGGDVTVKGARAADLGAFLDA
                     LVRAGAEVEALPGAVRVRSPRPLVATDVTTEPHPGFPTDLQAQFLALMTQASGTSRIT
                     ETIFENRFLHAGELLRLGADIRLDGRTAIVRGPTPLEGAPVTASDLRASAALVLAGLV
                     AKGETRVRRIYHLDRGYADMDGRLRSLGAHVERIDG"
     gene            complement(1739..>2250)
                     /locus_tag="PLB01_19600"
     CDS             complement(1739..>2250)
                     /locus_tag="PLB01_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HQQ79559.1"
                     /translation="HLADLGTGSGILAVSLALERPRARVLALDRSPAALALAGANARR
                     HGVADRVRPVASDWLSALGRAARFDLVVANPPYVPLTDQPHLSKTVSEHEPGLALYGG
                     PDGLDPLRVILAALPRFLEPGAPFVFEFGYSQANEVSALVEAAPLFRLERIRLDAAGI
                     PRTATAIRT"
BASE COUNT          313 a          776 c          897 g          264 t
ORIGIN      
        1 ctcggcggcc gcgagcggcg aggcgaggag ggcggcgagc aggacggtcg ggaggctcgg
       61 cctcaaccgg gcggccacgc gaacgagcgc ccgcccatga ggtgaaagtg cagatggaag
      121 acgctctggc ccgcgtccgg cccgtcgttc gcgacgaccc ggtagctcga gaggccgagc
      181 gtgcgcgcga tctccgcgac cgccgccagg cacgcgccga gcgtctccgc gtgcgccgcc
      241 gtcgtacccg agagggccgc gacgtgcacc ttcgggatca cgaggacgtg agtgggcgac
      301 ttcggggcga cgtcgtggaa ggcgaggacg tggtcgttct cgaacgcgat cctcgcggga
      361 gtctcccgcc ggccgatccg gcagaagagg cagctggggt cggcaaatgt cgggctcacg
      421 cggggattat ccgtcgatcc gctcgacgtg cgccccgagg ctccggaggc gcccgtccat
      481 gtccgcgtaa ccgcggtcga ggtggtagat ccgcctcacg cgcgtctcgc ccttcgcgac
      541 gaggcccgcg aggacgagcg ccgccgaggc gcggaggtcc gacgccgtca cgggcgcgcc
      601 ctcgagaggc gtgggcccgc gcacgatcgc ggtgcggccg tccagccgga tgtcggcgcc
      661 caggcgcagg agctcgcccg cgtggaggaa gcggttctcg aagatcgtct ccgtgatgcg
      721 gctcgtgccc gaggcctgcg tcatgagcgc gaggaactgc gcctgcagat ccgtcgggaa
      781 gcccgggtga ggctcggtcg tgacgtccgt ggcgacgagc ggccgggggg agcggacgcg
      841 cacggctccg gggagcgcct ccacctcggc gcccgcccgc acgagcgcgt cgaggaacgc
      901 gcccagatcc gccgcgcgcg ctcctttcac ggtgacgtcg ccccccgtga tcgcgccggc
      961 ggcgaggtac gtaccggcct cgatgcggtc gggcacgacg gcgtgcggcg cttcgccggt
     1021 gccccggaga cgctcgacgc cctcgaccgt gatcgtcgag ttcccggcgc ccgagacgtt
     1081 cgcgcccatc cggacgagga gacgggcggt gtcctcgacc tccggctccc gcgcggcgtt
     1141 ctcgatgacg gtcgtcccgc gcgcgagcgc ggcggcgaag aggacgttct cggtgcccgt
     1201 gacggtcggc gtcgggaatc gcaccgtcgc gccggtgagg cggccgcgcg cgcgagacac
     1261 gcgcgagacg tgggcgtgga tgtatccctt ctcgaccgcg acgcgcgcgc ccatcgcttc
     1321 gagcgccatg aggtggaggt cgaccggcct cacgccgatc gcgcagccgc cggggaggga
     1381 gacgcgcgcc tcgccgaagc gcgcgacgag cgggccgaga acgaggatcg acgcgcgcat
     1441 cgtgcggacg aggtcgtacg gcgcctccgt cgacgtgact cgcgaggcgt cgaggacgac
     1501 gcgtccgggc gcgggacggc cgagggagac gcccatgccg gcgacgaggc gttccatcgt
     1561 gcgcacgtcc gcgaggtccg gcacgccgtc gaggacgacg ggcgcgtccg cgaggagcgc
     1621 cgccgccatc tcggggagcg cggcgttctt ggccccgctc gcggagacgg tgccctccag
     1681 gcgcacgggg cctttcagca ggaacgcgtc cacggcaggg aagtctagcg gctcgcggtc
     1741 acgtccggat cgccgtcgcg gtgcgcggga tgcccgccgc gtcgaggcgg atgcgctcga
     1801 gtcggaagag gggcgcggcc tcgacgaggg ccgaaacctc gttcgcctgg ctgtacccga
     1861 actcgaagac gaacggcgcg cccggctcca ggaagcgggg aagcgccgcg aggatcacgc
     1921 gcaacgggtc gagtccgtcc gggccgccgt aaagcgcgag gccgggctcg tgttccgaga
     1981 cggtcttcga gaggtggggc tggtccgtga gcgggacgta cggaggattc gcgaccacga
     2041 ggtcaaagcg cgcggccctg ccgagcgcgg aaagccagtc ggacgcgacg gggcggacgc
     2101 ggtccgcgac gccgtgtcgc cgcgcgttcg cgcccgcgag agcgagggcg gcgggggagc
     2161 ggtcgagcgc gaggacgcgc gcgcgcggcc gttcgagcgc gagggacacc gcgagaatgc
     2221 cgctcccggt cccgaggtcc gcgaggtgcg
//
LOCUS       DAOMFY010000428         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_23907_length_2240_cov_8.000458, whole genome shotgun sequence.
ACCESSION   DAOMFY010000428 DAOMFY010000000
VERSION     DAOMFY010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23907_length_2240_cov_8.000458"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLB01_19605"
     CDS             <1..694
                     /locus_tag="PLB01_19605"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQQ79560.1"
                     /translation="GVSVVSGFARGVDAAAHRAALETEGSTVAVFGCGIDICYPAEQR
                     GLLETLLSTGTALSEFPMGDEPEPWHFPVRNRLIAGLSRIVCVVEAAEKSGSLITARC
                     AAGYGRDVAAVPGSVLAPGAAGSNALLKDGAILVRSTADLLAELPEGDRRRLNLVAAA
                     RTAASLPDLPPDAAALLGALDPDEPRDADALIPAAGLDAARFSAALLHLEIEGLATAL
                     PGALFVRSRPRS"
     gene            722..>2240
                     /gene="topA"
                     /locus_tag="PLB01_19610"
     CDS             722..>2240
                     /gene="topA"
                     /locus_tag="PLB01_19610"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQQ79561.1"
                     /translation="MAKSLVIVESPAKAATLGKFLGKDFTVMACYGHVRDLPRKGIAV
                     DREHGYTPTYEILPGKERTVADLRRAAKGADTIYLAADPDREGEAICWHLHEVLKPGA
                     PKAKFHRAEFHEITKAAVTRAVQNPGVIDQNRVNAQQARRIIDRLVGYEVSDLLWSKV
                     WRGLSAGRVQTVTLRIIVERESERDRFVAVPYFSVPVSLKKGDAAFPARVVAWKGEKL
                     RFDGTDPRLATAGEAEAARAHVAAATPVAVSIEAKERRSSPPPPFTTSKLQQGAARSL
                     GFSVRRTMQVAQRLYEGKTIGEKGSIGLITYMRTDSTRTAEEALAAVRDFIGRTYGGS
                     ALPEEARRFRQKKDAQDAHEAIRPTSMDLPPEAIARHLSPEELKLYRLIWNRFVASQM
                     SVSLSEITTVEFEARRPGSPDLAVLRATGSVLKDAGWLRVYEKEADTGEDEAPAEGEE
                     GDAAKVRLPALAEGDAVAVAEAAAEGHETQPPPRFNEASLVKFLEENGIGRPSTYA"
BASE COUNT          323 a          819 c          804 g          294 t
ORIGIN      
        1 cggggtctcg gtcgtttcgg gcttcgcgcg cggcgtggac gcggcggcgc accgcgcggc
       61 actcgagacc gagggctcca cggtcgccgt gttcggatgc ggcatcgaca tctgctaccc
      121 cgccgagcag cgcgggctcc tcgagaccct cctctctacg gggacggcac tctcggagtt
      181 tccgatgggc gacgagcccg agccgtggca cttcccggtg cgcaaccgcc tcatcgcggg
      241 gctgtcgcgg atcgtctgcg tcgtcgaggc cgcggagaag agcggatcgc tcatcacggc
      301 gcgttgcgcc gcggggtacg ggagggacgt cgccgcggtg ccgggttcgg ttctcgcgcc
      361 gggagccgcg ggctcgaacg cgctcctgaa ggacggggcg attctcgtgc gctcgaccgc
      421 cgacctcctc gccgaactgc ccgaggggga ccgccgccgg ctgaacctcg ttgcggcggc
      481 ccggaccgcg gcgtcgctcc ccgacctccc gccggacgcg gcggcccttc tcggggcact
      541 cgaccccgac gagccgcgcg acgccgacgc cctgattccg gcggcagggc tcgacgcggc
      601 gcgcttctcc gcggcgctcc tgcacctcga gatcgagggt cttgccaccg cccttccggg
      661 cgcgctcttc gtccgctcgc gtccccggag ttgacaggcg tggcatcgtg acgggtcccg
      721 tatggccaag tcactcgtga tcgtcgagtc ccccgcgaag gccgcgacgc tcggaaagtt
      781 cctcgggaag gacttcaccg tcatggcctg ctacggccac gtgcgcgacc tgccgcggaa
      841 gggcatcgcg gtggaccgcg agcacggcta cacgccgacc tacgagatcc ttcccgggaa
      901 agagcgcacc gtcgcggatc tccggcgggc cgcgaagggc gccgacacga tctacctcgc
      961 ggccgacccc gaccgcgagg gcgaggcgat ctgctggcat ctccacgagg tcctgaagcc
     1021 cggggccccg aaggcgaagt tccaccgcgc cgagttccac gagatcacga aggccgcggt
     1081 cacgcgggcg gtccagaacc cgggcgtgat cgaccagaac cgcgtgaacg cgcagcaggc
     1141 tcgccggatc atcgaccggc tcgtcggcta cgaggtgtcg gacctcctgt ggtcgaaggt
     1201 ctggcgcggc ctctcggcgg gccgggtcca gacggtcacg ctccggatca tcgtggagcg
     1261 cgagagcgag cgcgatcgct tcgtcgccgt gccgtacttc tccgtccccg tctccctgaa
     1321 gaagggcgat gcggccttcc cggcgcgcgt cgtcgcgtgg aagggcgaga agctgcgttt
     1381 cgacgggacc gacccgcgcc tcgcgaccgc cggcgaggcc gaggcggcgc gcgcccacgt
     1441 cgcggcggcg acgccggtcg ccgtgagcat cgaggcgaag gaacgccgct ccagcccgcc
     1501 gccgccgttc acgacgtcca agctccagca gggcgccgcg cgctccctcg ggttcagcgt
     1561 ccgccggacc atgcaggtcg cgcaacggct ctacgagggc aagacgatcg gcgagaaggg
     1621 ttcgatcggc ctcatcacgt acatgcgcac ggattcgacg cgcacggccg aggaagcgct
     1681 cgccgcggtc cgcgacttca tcggaaggac ttacggagga agcgcgctcc cggaggaggc
     1741 ccgccgcttc cgccagaaga aggacgcgca ggacgcgcac gaggcgatcc gcccgacgag
     1801 catggacctc ccgccggagg cgatcgcgcg ccacctgtcg cccgaggagc tcaagctcta
     1861 ccggctgatc tggaaccgat tcgtcgcgtc gcagatgagc gtgtcgctct cggagatcac
     1921 gacggtcgag ttcgaggcgc gccgccccgg ctcgccggac ctcgcggtcc tgcgcgcgac
     1981 gggctccgtc ctcaaggacg cgggctggct ccgcgtctac gagaaggaag ccgacacggg
     2041 cgaggacgag gcgcccgccg agggcgagga gggcgacgcc gcgaaggtgc gcctccccgc
     2101 gctcgccgag ggcgacgccg tcgccgtcgc cgaggcggcg gccgagggcc acgagacgca
     2161 gccgccgccg cggttcaacg aggcctcgct cgtgaagttc ctcgaggaga acggcatcgg
     2221 ccgcccgtcc acctacgccg
//
LOCUS       DAOMFY010000429         2239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_23926_length_2239_cov_6.388278, whole genome shotgun sequence.
ACCESSION   DAOMFY010000429 DAOMFY010000000
VERSION     DAOMFY010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23926_length_2239_cov_6.388278"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PLB01_19615"
     CDS             <1..1237
                     /locus_tag="PLB01_19615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQQ79562.1"
                     /translation="GAGPAGCSAATARAQRGRSVLLLDRDPEPRFKIGESLLPWNTPI
                     FEELGLLAKIEGAGFQRKYGALFTNERTGGTRQVDFRRAWDGSKPSAWQVKRLDFDGL
                     LAVHAAESGATVRRGAAVEDVRFDGARATGVRIAGAGAREEIEAKVVVDATGPAALLA
                     SHLKIRRQDTKLRRAALYAHYTGVWRGEGDRAGDILLPFRPDVWYWVIPFADGSASVG
                     AVFDPSLLGSLAGRTKAERLDEILALSAAMRGYLAGAVRTGDVGSVSDYSFTSTRHGG
                     DGWVLAGDAATFLDPVFSTGVFLGMSAGLRAAKAIDAALSAKGRVDASDFDAYGRTTD
                     RMVARFRPFVYGYYDPVFTRMFCEKEPFDALRAAVTSTLAGDVESPSVGVRFFNRLTL
                     MVFAFSRLVEKDEGVSATA"
     gene            complement(1161..1757)
                     /gene="folK"
                     /locus_tag="PLB01_19620"
     CDS             complement(1161..1757)
                     /gene="folK"
                     /locus_tag="PLB01_19620"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQQ79563.1"
                     /translation="MRARPARPRSPHPRPVFVAIGLGSNEGRREDLLRRSIAALAAHL
                     EGLRSSPFYESSPVPSFPPQPDFLNAVVVGRTKLSPGALLTLLRKSETAAGRKRAREA
                     RNGPRPLDLDILLYGNQTIASRSLTVPHPRMVSRLFVLVPLADLAPRRVVPGTAKTVA
                     RLLAEARGVSSDVVRPWRRRPRPSRRGARTRTPSASGD"
     gene            complement(1759..2088)
                     /locus_tag="PLB01_19625"
     CDS             complement(1759..2088)
                     /locus_tag="PLB01_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HQQ79564.1"
                     /translation="MAELGTKHTCFKCGTKFYDLKKNPVLCPKCGADQKDAPPQPTSP
                     VVLHAARRAKRDEFADPDVFDRNREEEDVVSEPEDPAAKRVDDDEELPLGEAPTLEGA
                     EESDDEY"
BASE COUNT          305 a          751 c          826 g          357 t
ORIGIN      
        1 cggcgccggc cccgcgggct gctccgccgc gacggcgcgc gcgcagcgcg ggcggtccgt
       61 tctcctcctc gaccgcgacc ccgagccgcg cttcaagatc ggcgagtcgc tcctcccgtg
      121 gaacacgccg atcttcgagg agctcgggct gctcgcgaag atcgaaggcg cgggcttcca
      181 gagaaagtac ggcgcgctct tcacgaacga gcggacgggc ggcacgcggc aggtggactt
      241 ccgcagggcc tgggacggct cgaaaccctc cgcatggcag gtcaaacgtc tggacttcga
      301 cgggcttctc gcggtgcacg cggcggagtc cggcgcgacg gtgcgccgcg gcgcggcggt
      361 cgaggacgtc cgcttcgacg gggcgcgggc gacgggtgtt cggatcgccg gcgccggcgc
      421 gcgggaggag atcgaggcga aggtggtcgt ggacgcgacc gggccggccg ccttgctcgc
      481 gtcgcacctc aagatccgcc gccaggacac gaagctccgg cgcgccgcgc tctacgcgca
      541 ctacacgggc gtctggcgcg gcgaggggga ccgcgccggg gacatcctgc tgcccttccg
      601 gccggacgtc tggtactggg tgatcccgtt cgctgacggc agcgcctcgg tgggcgcggt
      661 cttcgatccc tctctcctcg gctcgctcgc cgggaggacg aaggccgaga gactcgatga
      721 gattctcgcg ctctccgcgg cgatgcgcgg ttacctcgcg ggcgcggtgc gcacgggcga
      781 cgtcgggtcc gtctcggact actcgttcac gtcgacgcgc cacggcggcg acggctgggt
      841 gctcgcgggc gacgccgcga cgtttctcga ccccgtgttc tcgacgggcg tcttcctcgg
      901 catgtcggcc ggcctcagag ccgcgaaggc gatcgacgcg gcgctctccg cgaaagggcg
      961 cgtggacgcg tccgacttcg acgcgtacgg ccggacgacg gatcgcatgg tcgcgcgctt
     1021 ccgcccgttc gtgtacggct actacgaccc cgtcttcacg cggatgttct gcgagaagga
     1081 gccgttcgat gcgctgcgcg cggccgtgac gtccacgctc gcgggcgacg tggaaagccc
     1141 gtccgtcggg gtgcggttct tcaatcgcct gacgctgatg gtgttcgcgt tctcgcgcct
     1201 cgtcgagaag gacgagggcg tctccgccac ggcctgacga cgtcggacga gacgccccgc
     1261 gcctccgcga gcaggcgagc gacggtcttc gccgtgcccg gcacgactcg ccgcggcgcg
     1321 aggtcggcga gggggacgag gacgaagagg cgggagacca tgcgcgggtg gggaacggtc
     1381 aggctgcggc tcgcgatcgt ctggtttccg tagagaagga tgtcgaggtc cagggggcgc
     1441 gggccgttcc gggcctcacg cgctcgcttc cggccagcgg cggtttcgga ttttcttaga
     1501 agggttaaga gggccccggg cgagagcttc gttcgtccca cgacgaccgc gttgagaaag
     1561 tcgggctgag ggggaaaaga cgggacggga gaggattcat agaaaggtga agaacggagc
     1621 ccctcgaggt gcgcggccag cgccgctata gaccttctaa gaagatcttc tcttcgtccc
     1681 tcgttcgacc cgaggccgat ggcgacgaag accggccgcg ggtgcgggga tcgcgggcgc
     1741 gcggggcgcg cccgcacgtc agtactcgtc gtccgactct tccgcgcctt ccagcgtcgg
     1801 agcctcgccg agcgggagct cctcgtcgtc gtcgacgcgc ttggccgcgg ggtcctcggg
     1861 ctcggagacg acgtcctctt cctcgcggtt gcggtcgaag acgtccgggt ccgcgaattc
     1921 gtcgcgcttc gcgcgccgcg cggcgtgcag gacgacggga gacgtcggct gcgggggcgc
     1981 gtccttctga tccgctccgc acttcggaca caggacgggg ttcttcttga gatcgtagaa
     2041 cttcgtcccg catttgaagc acgtgtgttt cgttcccagt tcggccatga gcctcccgaa
     2101 aagacgccgg aacatagcat ccgtgttttt ttcggtcaac gcggcccggc acgtacactc
     2161 ggccggacca tgggcgagtg ctaccgaacc tcccgccggc cgggccgggc cggcctcgcg
     2221 gccgcggcga tcctggccg
//
LOCUS       DAOMFY010000430         2233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_24043_length_2233_cov_8.366391, whole genome shotgun sequence.
ACCESSION   DAOMFY010000430 DAOMFY010000000
VERSION     DAOMFY010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24043_length_2233_cov_8.366391"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="PLB01_19630"
     CDS             complement(<1..1803)
                     /locus_tag="PLB01_19630"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79565.1"
                     /translation="MTTTSDDRDPRRRPPLKKAAALATLALAIIVSGCAGSRAVQEAR
                     REEAREHWDLAVLAWEQAVALEPTLTELRVGLMRAKMKAAQIHYERGRIHRNSGQLDL
                     AVAELEQSVALDSTSDAAQMELKRAKADIEARARENAGGTPVEKAKARTRGARGAPPM
                     LVPSSTKPIDVTFPPDTNIKKIYAALGAAAGINVIYDPQLKDDRFSLDLRGMTFQKAL
                     ETTLRQAGHFYKVIDERTILIAQDTQQNRKEYEDLVIRTFFLSNGDVKDVSQMVRSIL
                     DLRRLAVIPQLNAVVIRDTADKVSVAERLIEVNDKAKSEVVVDVELLQINTQQIIDLG
                     ARYARTVTATVPTPGSTGTLPWEAIKNLGINDFMFSVPSITFSFIKDNTDAELLAKPQ
                     LRIAEGEKAQLVIGDRVPIPVTSINTQQAIGQQGVIPITSFQYQDVGIKIDMEPRVHH
                     NKEVSLKLTVEVSQINGYVSTGDANSPKQPIIGTRTITSSIRLKDGETNFLAGLIRKD
                     VSKTRNSIPFLGDIPLIGTLFSAVSTDTKRTDVFLTLTPHIIRSPQITDEDLIPIWVG
                     TENNVSFSGLNTRLESPNAAGSPFDAPALEAPRAA"
     gene            complement(1826..>2233)
                     /locus_tag="PLB01_19635"
     CDS             complement(1826..>2233)
                     /locus_tag="PLB01_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79566.1"
                     /translation="DLVQIPPRGARAGEVDRNVFRFYDKPTPTPTPVPTPRPTPIAPG
                     SPLFVGPMPIPPTPTPTPIVPPAIPYKVLGKFGPRDNPIVALEEGGRIINAREGDVVD
                     GRFRIHKINRESVDIAFPGLPPEITRRIPIPLP"
BASE COUNT          305 a          683 c          831 g          414 t
ORIGIN      
        1 cgccgcacgg ggagcctcga gggcgggcgc atcgaaaggc gagcctgccg cgttcggaga
       61 ctcgaggcgc gtgttcaggc ccgagaagga gacgttgttc tccgtcccga cccagatcgg
      121 gatgaggtcc tcgtccgtga tctgcggcga gcggatgatg tggggcgtca gcgtcaggaa
      181 gacgtccgtc cgcttcgtgt ccgtgctcac ggccgagaac agagtcccga tgagcggaat
      241 gtctccgagg aacgggatcg agttccgcgt cttggagacg tccttgcgga tgaggcccgc
      301 gaggaagttc gtttcgccgt ccttgagccg gatcgaggac gtgatcgttc gcgtcccgat
      361 gatgggctgc ttggggctgt tggcgtcccc cgtcgagacg tagccgttga tctgcgagac
      421 ctcgaccgtg agcttgagcg agacctcctt gttgtggtgg acgcgcggct ccatgtcgat
      481 cttgatgccg acgtcctggt attggaacga cgtgatcggg atgacgccct gctgcccgat
      541 ggcctgctgc gtgttgatcg acgtgacggg gatcgggacg cggtcgccga tgacgagctg
      601 ggccttctcc ccctcggcga tgcgcagctg cggcttggcg aggagctcgg cgtccgtgtt
      661 gtccttgatg aagctgaacg tgatggacgg gaccgagaac atgaagtcgt tgatcccgag
      721 gttcttgatc gcttcccagg ggagcgtccc cgtcgagccg ggggtcggga ccgtcgccgt
      781 gaccgttctt gcgtaccgcg cgccgaggtc gatgatctgc tgggtgttga tctggaggag
      841 ctcgacgtcg acgacgacct cggacttcgc cttgtcgttc acctcgatga ggcgctcggc
      901 gaccgagacc ttgtcggccg tgtcgcggat gacgacggcg ttgagctgcg ggatcaccgc
      961 gaggcgcctc aggtcgagga tcgaccggac catctgcgag acgtccttca cgtcgccgtt
     1021 cgagaggaag aacgtccgga tgacgaggtc ctcgtactcc ttgcggttct gctgcgtgtc
     1081 ctgcgcgatg aggatcgtcc gctcgtcgat gaccttgtag aaatggcccg cctgccggag
     1141 cgtcgtctcg agcgccttct ggaaggtcat gccccggagg tcgagcgaga accggtcgtc
     1201 cttgagctgc gggtcgtaga tgacgttgat gccggcggcg gcgccgaggg ccgcgtagat
     1261 cttcttgatg ttcgtgtcgg gcgggaacgt gacgtcgatg ggcttcgtcg acgacgggac
     1321 gagcatcggc ggcgcgccgc gcgcgccgcg cgtccgggcc ttcgccttct cgacgggggt
     1381 gccgcccgcg ttctcccgcg cgcgcgcctc gatgtccgcc ttcgccctct tcagctccat
     1441 ctgcgcggcg tcgctcgtgg agtcgagcgc gacggactgc tcgagctcgg cgaccgcgag
     1501 gtcgagctgg cctgagttgc ggtggatgcg cccgcgctcg tagtggatct gggcggcctt
     1561 catcttcgcc cgcatgaggc cgacccggag ctccgtcagc gtcggctcga gcgcgacggc
     1621 ctgctcccag gcgaggacgg cgaggtccca gtgctcgcgc gcttcctccc gccgggcttc
     1681 ctgaacggcg cgcgagccgg cgcagccgga gacgatgatg gccagcgcca acgtggcgag
     1741 cgccgccgcc ttcttcagcg gcgggcggcg gcgggggtcg cgatcgtcag aagtcgtcgt
     1801 catgcgaagt ccttcggcgt gcccgtcatg gcagcggaat ggggatcctg cgtgtgatct
     1861 cgggcggaag gcccgggaag gcgatgtcga cggattcgcg gttgatcttg tggatccgga
     1921 accgcccgtc gaccacgtcg ccctcgcggg cgttgatgat gcgtcccccc tcctcgagcg
     1981 cgacgatcgg gttgtcgcgc ggcccgaact ttcccagcac cttgtacgga atggccggcg
     2041 gaacgatcgg ggtcggggtg ggcgtcggcg gaatgggcat cggccccacg aagagaggcg
     2101 agcccggagc gatgggcgtc gggcgcgggg tcgggacggg cgtgggcgtc ggcgtcggct
     2161 tgtcgtagaa gcggaagacg ttgcgatcca cctcgccggc gcgggcgccg cgcggcggga
     2221 tctgtacgag gtc
//
LOCUS       DAOMFY010000431         2233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_24044_length_2233_cov_6.267218, whole genome shotgun sequence.
ACCESSION   DAOMFY010000431 DAOMFY010000000
VERSION     DAOMFY010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24044_length_2233_cov_6.267218"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1769)
                     /locus_tag="PLB01_19640"
     CDS             complement(117..1769)
                     /locus_tag="PLB01_19640"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HQQ79567.1"
                     /translation="MTAEFTRDEAEILQRFFTNVDAPVFGLRLPQEVSGALFSRYSRT
                     AKSLRRVFLDEFVGGEGLAEGLLGTGAAADDGESLRRARAFYDRVLVGYGDDSVAQLG
                     AAHVAVERASNVAAQALEDARVGISFLEKSTRYVRFDQRDASGNFLWHREGTLMASRH
                     GAAFASLMDRLFATYAAQIDPMIRHIETILPVETVEVRHPKTGEPLPWRDVEKDEELL
                     KAARRAYGATVRAHACDVMRSYLPGATLTNLGVFATGQAFEHLLNRLFSSPLAECRDL
                     GASMHGELERLIPSFVKRAKRSDWLVDLDARARSAASRVLPPGSAPAGSVTLLDFDPE
                     GEAKVLAALLYPYSDRPLEEARARVAEMPAAERAALHAELAGARRNRRDKPPRALEET
                     AYAFDLVGNFGIYRDLHRHRLLSQERQPFTTANGFETPPEIVESGCESEFRARMEEAH
                     ALHEAAAREHPHEAQYVVPFAFKVRWRVTLNLREAIHLCELRSMPQGHPDYRLLVQEM
                     WKRIEAAHPALAPWGKFVNRDTYRLGRLQSEMRTEYKRAQTA"
     gene            complement(1790..>2233)
                     /locus_tag="PLB01_19645"
     CDS             complement(1790..>2233)
                     /locus_tag="PLB01_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79568.1"
                     /translation="GVALEGGEFRMTEEFTRHAATWVEAPASRVRAGRTVSVNVRLPK
                     GWSLGAVEVAHERFPRPLTAKEIRKRSSYAYPKASQTLLPLLAPGFQYSSGGKGDVAV
                     ADGVARAKIALASGPGSYWVLVYAAPGAVDGKTLSPITAVRILAD"
BASE COUNT          303 a          803 c          807 g          320 t
ORIGIN      
        1 tggtcacgga gatcttccac gagatccccg gcgcgatccc cgtctacctc gcgcgtcccg
       61 acgaggacgc cgcccaggtc ctggccggcc tcgacgagct gacggcgggc tgacgcctag
      121 gccgtctgcg cccgcttgta ctccgtccgc atctcggact ggaggcggcc gaggcggtac
      181 gtgtcgcggt tcacgaactt cccccagggc gcgagcgcgg ggtgggccgc ctcgatccgc
      241 ttccacatct cctggacgag gaggcggtag tccgggtgcc cctgcggcat cgagcgcagc
      301 tcgcagaggt ggatcgcctc gcggagattc agcgtcacgc gccagcgcac cttgaacgcg
      361 aacggcacca cgtattgcgc ctcgtgcggg tgctcgcggg cggccgcctc gtggagcgcg
      421 tgcgcctcct ccatccgcgc acggaactcg ctctcgcagc cggactcgac gatctcgggc
      481 ggcgtctcga agccgtttgc cgtcgtgaac ggctgccgct cctgcgagag gaggcgatgg
      541 cggtggaggt cgcggtagat cccgaagttc ccgacgaggt cgaatgcgta ggccgtctct
      601 tcgagcgcgc gcggaggctt gtcgcggcgg ttgcgccgcg cgcccgcgag ctccgcgtgg
      661 agcgcggcgc gctcggccgc cggcatctcg gcgacgcgcg cgcgggcctc ctcgagcggc
      721 cggtcggagt acgggtagag gagcgccgcg aggaccttcg cctcgccttc gggatcgaag
      781 tcgaggagcg tgacggagcc ggcgggcgcg gagccgggcg gcaggacccg cgaggcggcg
      841 ctgcgggcgc gcgcgtcgag gtccacgagc cagtcgcttc tcttcgcgcg cttcacgaac
      901 gacgggatga gcctttcgag ctctccgtgc atggacgccc cgaggtcgcg gcactcggcg
      961 agcgggctcg agaagaggcg gttcaggagg tgctcgaagg cctgccccgt cgcgaagacg
     1021 ccgaggttcg tgagcgtggc ccccggcagg tagctccgca tgacgtcgca tgcgtgcgcg
     1081 cgcaccgtgg ccccgtaggc gcgccgcgcc gccttcagga gctcctcgtc cttctcgacg
     1141 tccctccacg ggagcggctc tcccgtcttc gggtggcgca cctcgacggt ctcgaccggg
     1201 aggatcgtct cgatgtgccg gatcatcggg tcgatctgcg ccgcatacgt cgcgaaaagc
     1261 cggtccatga gcgacgcgaa ggcggcgccg tggcgcgagg ccatcagcgt tccctcgcgg
     1321 tgccagagga agttcccgga ggcgtcgcgc tggtcgaacc ggacgtaccg ggtggacttc
     1381 tcgaggaacg agatcccgac gcgcgcgtcc tcgagcgcct gcgccgcgac gttggaggcg
     1441 cgctcgaccg cgacgtgcgc cgcgccgagc tgcgcgacgg agtcgtcgcc gtagcccacg
     1501 aggacgcggt cgtagaaggc gcgcgcgcgc cggagcgact cgccgtcgtc cgcggcggcc
     1561 cccgtgccga gaaggccctc ggcgaggcct tccccgccga cgaactcgtc gaggaagacc
     1621 cggcgcaggc tcttcgcggt ccggctgtag cgcgagaaga gcgcgcccga gacctcctgc
     1681 ggaagccgga gaccgaacac gggcgcgtcg acgttcgtga agaagcgctg cagtatctcc
     1741 gcttcgtccc gcgtgaactc cgccgtcatg ttcccgcgat cttagaccgt cagtccgcga
     1801 ggatccgcac cgccgtgatc gggctcagcg tcttcccgtc gacggcgccc ggcgccgcgt
     1861 agacgaggac ccagtagctt cccggcccag aggcgagcgc gatcttcgcg cgcgccacgc
     1921 cgtcggcgac ggcgacgtcc cccttcccgc cgctcgagta ctggaacccg ggcgcgagga
     1981 gcggaaggag cgtctgcgag gccttcgggt acgcgtacga gctgcgcttc cggatctcct
     2041 tcgccgtgag cggccgcgga aagcgctcgt gcgccacctc gaccgccccc agcgaccatc
     2101 ctttcgggag ccggacgttc acggacacgg tgcggcccgc acgcacgcgg gaggccggag
     2161 cctcgaccca cgtcgccgcg tggcgcgtga actcctcggt catccggaac tcgccgccct
     2221 cgagcgcgac gcc
//
LOCUS       DAOMFY010000432         2223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_24224_length_2223_cov_9.130996, whole genome shotgun sequence.
ACCESSION   DAOMFY010000432 DAOMFY010000000
VERSION     DAOMFY010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24224_length_2223_cov_9.130996"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLB01_19650"
     CDS             complement(<1..286)
                     /locus_tag="PLB01_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79569.1"
                     /translation="MNTGAFSRWREARAETRRFFDETLTPGAYEARPIALRHPFVFYE
                     GHLAAFGAIKLLREALGRPPLDAVLEKLFERGIDPPAEGGEAPAVAWPARE"
     gene            complement(291..2075)
                     /locus_tag="PLB01_19655"
     CDS             complement(291..2075)
                     /locus_tag="PLB01_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HQQ79570.1"
                     /translation="MRDGVKLHTVILVPKGAKNAPMLLTRTPYDADALTKRNESAHLG
                     MALDGYDNPVETVVEGGYIRVVQDVRGKHGSEGDYVMNRPLHGPQNPTPVDHATDTWD
                     TIDWLVKNVPESNGKVGILGISYNGFTPLMALVNPHPALKCSVPMNPMVDGWRGDDWF
                     HNGAFRQIGGLSYHYDQEASKKSDVKWWSSHYDDYTAYLEAGSAGEMGRRRGLEQVGM
                     WRKILAHPSYDAFWQDQALDRILAAQPLKVPVLIVHSLFDQEDIYGAPAVYKAIEPKD
                     ARNDMVYLVLGPWHHGQEIREGSALGAVKWDSDTALWFRRNVLTPFLDEHLKDGAPKS
                     GIAPVTAFETGTNTWRRLNAWPAGCESGCAPKATSLFLGAGQKASFEAPKAGAPAFSE
                     YVSDPAKPVPFRGRPYPVSSYESNTAWRTWLVTDQREVATRPDVLVFETEVLTAPVKI
                     AGEPVVKLVASTSGTDSDWVVKLIDVYPPEVPLQPAMGGYQLPVAMDVFRGRYRESLE
                     TPKALESGKPLAYTFALPNANHVFKKGHRIMVQVQSTWFPLYDRNPQTFVPSIFWAKP
                     ADYVKATQRVFHAPGQASLVELPVVTAP"
BASE COUNT          309 a          779 c          775 g          360 t
ORIGIN      
        1 cctcgcgcgc gggccacgcg accgccggcg cctcgccgcc ctcggccggc gggtcgatgc
       61 cgcgctcgaa gagcttctcg aggacggcgt cgagcggcgg gcgcccgagc gcctcccgaa
      121 gcagtttgat cgccccaaag gccgcgagat ggccttcgta gaagacgaac gggtggcgca
      181 gcgcgatcgg gcgggcctcg tacgctccgg gcgtgagcgt ctcgtcgaag aaccgccgcg
      241 tctcggcccg cgcctcgcgc caccgggaga aggcgcccgt gttcatgtcg tcagggcgcc
      301 gtcacgaccg gcagctcgac gaggctcgcc tgccccggcg cgtggaagac ccgctgcgtc
      361 gccttcacgt agtcggccgg cttcgcccag aagatgctcg ggacgaacgt ctgcgggttg
      421 cggtcgtaga gcgggaacca cgtcgactgc acctggacca tgatgcggtg gcccttcttg
      481 aagacgtggt tcgcgttcgg gagcgcgaac gtgtaggcga gcggcttgcc gctctcgagc
      541 gccttcggcg tctcgaggct ctcgcggtac cgcccgcgga agacgtccat cgcgaccggc
      601 agctggtagc cgcccatcgc cggctgcagc ggcacctcgg gcgggtagac gtcgatgagc
      661 ttcacgaccc agtcggagtc cgtgccgctc gtcgaggcga cgagcttcac gacgggctcg
      721 cccgcgatct tgacgggcgc cgtcaggacc tccgtctcga aaacgaggac gtcgggccgg
      781 gtcgcgacct cgcgctggtc cgtcacgagc cacgtgcgcc aggccgtgtt gctctcgtag
      841 ctggaaaccg gatagggacg tccgcggaac ggcaccggct tggccgggtc ggagacgtac
      901 tcggaaaacg caggcgcgcc ggccttcggc gcctcgaaag acgccttctg gcccgccccg
      961 aggaagagcg aagtcgcctt cggcgcgcag ccgctctcgc agcccgcggg ccaggcgttg
     1021 aggcggcgcc acgtgttcgt gcccgtctcg aacgcggtca cgggcgcgat cccggacttc
     1081 ggagcgccgt ccttgaggtg ctcgtcgaga aacggcgtca gcacgttgcg gcggaaccag
     1141 agcgccgtgt ccgagtccca cttcacggcg ccgagcgcgc tcccctcgcg gatctcctgc
     1201 ccgtgatgcc acggcccgag cacgaggtag accatgtcgt tccgcgcgtc cttcggctcg
     1261 atcgccttgt agacggcggg cgcgccgtag atgtcctcct gatcgaagag gctgtggacg
     1321 atcaggacgg gcaccttgag cggctgcgcc gcgaggatgc gatccagcgc ctggtcctgc
     1381 cagaacgcgt cgtaggaggg gtgcgcgaga atcttccgcc acatcccgac ctgctcgagt
     1441 ccgcggcggc ggcccatctc gcccgccgag cccgcctcga ggtacgccgt gtagtcgtcg
     1501 tagtgcgagg accaccactt cacgtcgctc ttcttcgacg cttcctggtc gtagtggtac
     1561 gacagcccgc cgatctggcg gaacgcgccg ttgtggaacc agtcgtcgcc gcgccagccg
     1621 tccaccatcg gattcatcgg cacggagcac ttgagcgcgg gatgcgggtt cacgagcgcc
     1681 atgagcggcg tgaagccgtt gtacgagatc ccgaggatcc cgaccttccc gttcgactcg
     1741 gggacgttct tcacgagcca gtcgatcgtg tcccacgtgt ccgtcgcgtg gtcgacgggc
     1801 gtcgggttct gcggcccgtg gaggggccgg ttcatgacgt agtcgccctc ggagccgtgc
     1861 ttcccgcgca cgtcctggac gacgcggatg tacccgccct cgacgacggt ctcgaccggg
     1921 ttgtcgtacc cgtcgagcgc cattccgaga tgcgcgctct cgttcctctt cgtgagcgcg
     1981 tccgcgtcgt acggcgtgcg cgtcaggagc atcggcgcgt tcttcgcgcc cttcggcacg
     2041 aggatcacgg tatggagctt caccccgtcg cgcatcggga tctcggccgt gcgccgctcg
     2101 tggtcgaagc cgtcgaacgt cggctcgaac gtcgccgcga tctcgctcgg gcagtccggg
     2161 tactcccgcg gcgcgcgcgt cgccgctcgc cggcaacccc tcgagcgagc acgaggaggg
     2221 gcg
//
LOCUS       DAOMFY010000433         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_24633_length_2203_cov_5.542831, whole genome shotgun sequence.
ACCESSION   DAOMFY010000433 DAOMFY010000000
VERSION     DAOMFY010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24633_length_2203_cov_5.542831"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2203)
                     /locus_tag="PLB01_19660"
     CDS             complement(<1..>2203)
                     /locus_tag="PLB01_19660"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HQQ79571.1"
                     /translation="PLPLVEGPNVLAARAADAAGRSGEDRVVVFRDSAAPKIAITSPP
                     DGAHVGKPGVGAAAVNVSGVVDLTSEPNLASVVVSTGAGSVTATVDADAGTFWAGGVP
                     LVTTSPGAPQSVTATATDTLAQAGRATVSVVYDPAAPALVLAQPVDGARFSETSPASF
                     TASGEAWAKDGAIVSINGGGLDGLAWDAAAADGRRHAMFTAQVTVPAAEGPFGVIARV
                     EEPSGAYANDKRLLFKDVTAPTVVSLQPADGAKQVDANEIPLVLFSETVRPSSLSAAD
                     GLTLTRVATGDKAVGTFTVAGSAVAFVPGAAMVRGEVYRFRAGTGITDLAGHPLAAAK
                     EVRFTVAAVGTGAAPALDALPPVVCATQVVVSGTAAPNAAVRVRDAGLSFTGNADAAG
                     RFAVTLPLSGNGWHAVHAAVIDSGGSPGPEAAALFRVDCSAPSVAGAAFDRGTGKLTV
                     AFTEAMKPASLTVGGAGSAITVSKADDATNAPQAAALALSADGLTATLDLGGGASAWW
                     SGLSVRLSVGPPAADASGNAMAALYATVFFAGGGGDPSGGFLSGAVLDEESGRPLSSA
                     DVKLYASGAVLPGAAPPAQVTAPVASTVTDGRGRFSLTGDIPAGRYALVLSRAGYTRS
                     VRRLALEPAVGAVPFGSRLTPLAAQAASLLVPGTGGSFAGPAGSNAAVDFAANALASA
                     TNLGVRLTPLAGQGLPEPLPLGFTPLAAAELRLIPNGAGDDALPEGAGTPFA"
BASE COUNT          295 a          815 c          850 g          243 t
ORIGIN      
        1 cgcgaacgga gtgcccgcgc cctcgggcag cgcgtcgtca ccggcgccgt tcgggatcag
       61 gcgcagctcg gctgcggcga gcggcgtgaa gccgagcggc agcggctcgg gcagtccctg
      121 gcccgcgagc ggcgtcaggc ggacgccgag gttcgttgcg gacgcgagcg cgttcgccgc
      181 gaagtccacc gccgcgttcg agccggcggg gcccgcgaac gagccgcccg ttcccggaac
      241 gagaagcgac gcggcctggg ccgcgagagg agtcaggcgg ctgccgaacg gcaccgcgcc
      301 gacggcgggc tccagcgcga ggcgccgcac cgaccgcgtg tagcccgcgc gcgagaggac
      361 gagcgcgtaa cggccggccg gaatgtcgcc cgtgaggctg aaacgcccgc ggccgtcggt
      421 cacggtgctt gcaaccggcg ccgtgacctg cgcgggaggc gcggcgcccg gcagcacggc
      481 gcccgatgca tagagcttaa cgtccgccga cgagagcggc ctgccggact cctcgtccag
      541 cacggcgccg gagaggaagc cgcccgaggg atctcccccg ccgcccgcga agaagacggt
      601 cgcgtagaga gccgccatcg cgttgccgga ggcgtcggcc gccggcggcc cgacggagag
      661 gcgcacggag aggccgctcc accacgccga ggcgccgccg ccgagatcga gcgtcgcggt
      721 gaggccgtcc gccgagagcg ccagcgccgc cgcctgaggc gcgttggtcg cgtcgtccgc
      781 cttcgagacg gtgatcgcgc tgccggcgcc gccgaccgtg agcgacgcag gcttcatcgc
      841 ctccgtgaag gcgaccgtca gcttcccggt gccgcggtcg aacgcggctc cggcgacgga
      901 cggcgcgctg cagtccacgc ggaagagagc cgcggcttcc ggtccaggcg atccgcccga
      961 atcgatcacg gcggcgtgca cggcgtgcca gccgttgccc gagagcggga gcgtgacggc
     1021 gaaccggccc gccgcgtcgg cgtttccggt gaacgagagg ccggcgtcgc gcacgcggac
     1081 ggccgcgttc ggcgcggccg tgccggacac gacgacctgc gtcgcgcaga cgacgggcgg
     1141 cagcgcgtcg agcgcaggcg cggcgcccgt tcccaccgcc gcgaccgtga atcgcacttc
     1201 cttcgccgcg gcgagcggat gcccggcgag gtcggtgatc ccggtccccg cgcggaacct
     1261 gtagacctcg ccacgcacca tcgcggcccc gggcacgaac gcgaccgcac tgcccgccac
     1321 cgtgaacgtg ccgacggcct tgtcgcccgt cgcaacgcgc gtcagcgtga ggccgtccgc
     1381 ggccgagagc gaggatgggc ggaccgtctc gctgaagagg acgagcggga tctcgttcgc
     1441 gtcgacctgc ttcgccccgt cggcgggctg cagggagacg acggtgggcg cagtgacgtc
     1501 cttgaagagg agacgcttgt cgttcgcgta cgcgcccgag ggctcctcca cgcgcgcgat
     1561 gacgccgaac ggcccttccg ccgcaggcac ggtgacctgc gccgtgaaca tcgcatggcg
     1621 gcgcccgtcg gccgcggccg cgtcccacgc gaggccgtcg aggccgccgc cgttgatcga
     1681 gacgatggct ccgtccttcg cccaggcctc gcccgaggcc gtgaaagacg cgggcgacgt
     1741 ttcgctgaag cgcgcgccgt ccacgggctg cgcgaggacg agcgcgggcg ccgccgggtc
     1801 gtagacgacg ctgaccgtcg ccctgccggc ctgggcgagc gtgtcggtgg cggtcgccgt
     1861 gacgctctgc ggcgcgccgg gcgacgtcgt cacgagcggg acgccccctg cccagaatgt
     1921 accggcgtcg gcgtccaccg tcgcggtgac ggagcccgcg cccgtcgaga cgacgaccga
     1981 cgcgaggttc ggctcgctcg tgagatccac gacgcccgag acgttgacgg cggccgcgcc
     2041 cacgccgggc tttccgacgt gcgcgccgtc gggcggcgag gtgatggcga tcttcggcgc
     2101 cgccgagtcg cggaagacga cgacgcgatc ctcgccgctg cggcccgcgg cgtcggcggc
     2161 gcgcgccgcg aggacgttcg ggccctcgac gagcggcagc ggg
//
LOCUS       DAOMFY010000434         2201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_24659_length_2201_cov_6.755359, whole genome shotgun sequence.
ACCESSION   DAOMFY010000434 DAOMFY010000000
VERSION     DAOMFY010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24659_length_2201_cov_6.755359"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2201)
                     /gene="mfd"
                     /locus_tag="PLB01_19665"
     CDS             complement(<1..>2201)
                     /gene="mfd"
                     /locus_tag="PLB01_19665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQQ79572.1"
                     /translation="PARLAGDADRLAEDVARRTVLALAPAVPAGETVAVGAESVLSFE
                     DRLPDVPKEIARARRDGLAVVISAASKGEREHVERLLAEYDVDHQGADSDPTSPLAPG
                     ACRVVSGGPRTGFLFRSAGVLLLTASDLFGEPRTAAVRRKSASEAFLSDLRDLNVGDV
                     VVHRDYGLGLFAGLARIDDAGAVREMVDLRYAGDAKLLVPVERLDLIQKYASAGDGPS
                     PALDRLGGTGWEKRKSSVRKAVKDIADQLLKLYARRASAPGHAFSKDSPWQKEFEDAF
                     EYVETPDQAAAIRDVKRDMESEKPMDRLLCGDVGYGKTEVGMRAIFKCVLDGKQAAFL
                     APTTILADQHFRTLKRRFAAFPVSLDLLSRFRTKDEQKEVLRRLAEGTLDVVVGTHRI
                     LSKDVAFKDLGLLVVDEEQRFGVAQKERIKEWRASVDVLAMSATPIPRSLHLSLAGIR
                     DLSVIETPPKDRLAIDTHVVAMDDAVIREAIRAEVDRGGQVYVVHNRIESLGTWRERL
                     QALVPDVKVVSAHGQMSEGELERAMRAFVTRAADLLLATTIVENGLDIPSANTMIIDR
                     ADLYGLSQLYQLRGRVGRSDKAASCWLLVPPGMPLSDDARRRLRAIQEFSDLGAGFRI
                     AAKDLEIRGAGNVLGGEQSGHIESVGFETYVNLLEEAMAEMKGEPVPETRDVTLQLGV
                     PLALPASWIPEENLRMALYKRIAGAPDEATLAREASAAEDRYGAAPRAFRA"
BASE COUNT          314 a          776 c          795 g          316 t
ORIGIN      
        1 cgcccggaac gcccgcggcg ccgcgccgta gcggtcctcg gccgcggacg cctcgcgcgc
       61 gagcgtcgcc tcgtcgggag cgccggcgat ccttttgtag agcgccatcc gcaggttctc
      121 ctccggaatc cacgaggccg gaagggcgag cggaacgccg agctggagcg tcacgtcgcg
      181 cgtctcgggc accggctcgc ccttcatctc ggccatcgcc tcctcgagga ggttcacgta
      241 cgtctcgaag ccgacggact cgatgtgccc ggactgctcg cccccgagga cattgcccgc
      301 gccgcggatc tcgaggtcct tggccgcgat ccggaatccg gcgccgaggt ccgagaactc
      361 ctggatcgcg cgcaggcgcc gccgcgcgtc gtccgagagc ggcatccccg gcgggacgag
      421 gagccagcag ctcgcggcct tgtcgctgcg gcccacgcgt ccgcgcagct ggtagagctg
      481 cgagagtccg tagaggtcgg cgcggtcgat gatcatcgtg ttcgccgagg gaatgtcgag
      541 cccattctcg acgatggtcg tcgcgaggag aagatccgcg gcgcgagtga cgaaggcgcg
      601 catcgcccgt tcgagctcgc cctcggacat ctggccgtgg gcgctcacga ccttcacgtc
      661 cgggacgagc gcctggagcc gctcgcgcca cgtcccgagc gactcgatcc ggttgtggac
      721 gacgtacacc tgtccgccgc ggtccacctc ggcgcggatc gcctcgcgga tcacggcgtc
      781 gtccatcgcg acgacgtgcg tgtcgatcgc gaggcggtct ttcggaggcg tctcgatcac
      841 ggacaggtcg cggatgcccg cgagcgagag gtggagcgac cggggaatgg gcgtcgccga
      901 catggcgagg acgtcgacgg aggcgcgcca ctccttgatc cgctccttct gcgcgacgcc
      961 gaagcgctgc tcctcgtcca cgacgaggag gccgagatcc ttgaacgcca cgtccttcga
     1021 gaggatccgg tgcgtcccga cgacgacgtc gagcgtccct tccgcgagcc gccgcaggac
     1081 ctctttctgc tcgtccttcg tccggaagcg cgagagaagg tcgagcgaga ccggaaacgc
     1141 cgcgaagcgg cgcttgagcg tccggaagtg ctggtccgcg aggatcgtcg tcggggcgag
     1201 gaaggccgcc tgcttcccgt cgaggacgca cttgaagatc gcgcgcatcc cgacctcggt
     1261 cttgccgtag ccgacgtcgc cgcagaggag ccggtccatg ggtttctccg actccatgtc
     1321 gcgcttcacg tcccggatcg cggccgcctg gtccggcgtc tcgacgtact cgaacgcgtc
     1381 ctcgaattcc ttctgccacg gcgagtcctt cgagaacgcg tgtcccgggg ccgaggcgcg
     1441 gcgcgcgtag agcttcagga gctggtctgc gatgtccttg acggccttgc ggacggagga
     1501 cttccgcttc tcccagcccg tcccgccgag acggtcgagc gcgggcgaag gaccgtcgcc
     1561 cgcggaagcg tatttctgga tgaggtccag tcgctcgacc gggacgagga gcttcgcgtc
     1621 gcccgcgtag cgcaggtcga ccatctcgcg gacggcgccc gcgtcgtcga tgcgcgcgag
     1681 gcccgcgaag agcccgaggc cgtagtcgcg gtggacgacg acgtccccga cgttgaggtc
     1741 gcgcaggtcc gagagaaacg cctccgacgc cgacttgcgc cgcacggccg cggtccgcgg
     1801 ctcgccgaag aggtccgaag ccgtcaggag gagaacgccc gcggatcgga acaggaaccc
     1861 cgtgcgcggg ccgcccgaga cgacgcggca cgcgcccgga gcgagcggcg acgtcgggtc
     1921 ggagtccgcg ccctgatggt cgacgtcgta ctcggcgagg aggcgctcga cgtgctcgcg
     1981 ctcgcccttc gacgccgccg agatcacgac cgcgaggccg tcgcggcgcg cgcgggcgat
     2041 ctccttcggc acgtccggga ggcggtcctc gaaggagagg acggactcgg ctccgaccgc
     2101 gacggtctcg cccgcgggca cggcgggcgc cagcgcgagg acggtccgcc gcgcgacgtc
     2161 ctcggcgagc cggtccgcgt cgccggcgag acgcgcgggg t
//
LOCUS       DAOMFY010000435         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_24878_length_2191_cov_6.272940, whole genome shotgun sequence.
ACCESSION   DAOMFY010000435 DAOMFY010000000
VERSION     DAOMFY010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24878_length_2191_cov_6.272940"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2105)
                     /locus_tag="PLB01_19670"
     CDS             complement(<1..2105)
                     /locus_tag="PLB01_19670"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQQ79573.1"
                     /translation="MGLAGHTLSKKISLPPGDPAPYRVGERPVTGRRRRRLDGPEKVT
                     GRAVYTHDARKAGMLHARIVRSPHAHAKIVSIDVSRAEKMPGVVIENPGKKVVRYHGE
                     AVVALAAPTRAAAEDAMRAVKVTYDVLPHTVSLAAARKEGATLVFEKSVEEKRTAGDE
                     PGAAAALPQKGNVRGPKPSGKGDVEKGFAEAEGTLEIVTTTSVQTHTCMETHSMFAEW
                     VGDTLEVQASTQGTFSVRDELAEVFKLSKDKVIVRAEFTGGGFGSKFGARDYGVFTAK
                     LAKKAGRPVLMALERKEDQLSSGNRPDSWNRVKLGYRKDGTVTAADYESFGTAGVATG
                     TGTAGFVKAAYGFPAVRAAESDVFTHLGPGCAMRAPGHPQGCFAVETALEELASRLGM
                     DPLALRLKNDPSEVRRAEWEIGAKEFGWSRRAEITKANEAAAASGSPLRRGIGCAASL
                     WYTFVAPGSQVLVRIHRDGTVEVENGVQDIGGGVRTPIAMVVAEEMGLPVESLRVKIG
                     DSRFPFGPASGGSVTIGSLIPAVRAAAVHARERLVGVAAKVLGAAESDVRIAGGVFSA
                     KGQRADFRKVCARIPGDTLVATGDRAKDYDGADTRLFGVQFAEVVVDVETGVVVVKKV
                     VAVHDCGQPVWKTGVESQIRGGILQGISYALFEERIVDERSGRVMNPDVEFYKVLGSK
                     DTPEIVPILVDFYPGKNNA"
BASE COUNT          293 a          780 c          739 g          379 t
ORIGIN      
        1 tgcgcgttgt tcttgccggg gtagaagtcg acgaggatcg ggacgatctc cggcgtgtcc
       61 ttgctcccga gcaccttgta gaactcgacg tccggattca tcacgcggcc ggagcgttcg
      121 tccacgatgc gctcctcgaa gagcgcgtag ctgatgccct gcaggatgcc gccgcggatc
      181 tggctctcga cgcccgtctt ccacacgggc tggccgcagt cgtgcaccgc gacgaccttc
      241 ttcacgacca cgaccccggt ctcgacgtcc acgacgacct cggcgaactg cacgccgaag
      301 agccgcgtgt cggcgccgtc gtaatccttc gcgcggtcgc cggtcgcgac gagcgtgtcg
      361 ccgggaatac gcgcgcagac cttcctgaag tcggccctct gtcccttcgc ggagaagacg
      421 ccgccggcga tccgcacgtc ggattccgcg gcgccgagga cctttgcggc gacgcccacg
      481 agcctttcgc gggcgtgaac ggcggcggca cgcacggccg ggatcagaga gccgatcgtc
      541 acggagccgc ccgacgccgg gccgaacggg aagcgcgagt cgccgatctt cacgcggagg
      601 gactcgaccg ggagtcccat ctcctccgcc acgaccatcg cgatcggcgt ccggactccg
      661 cctccgatgt cctgcacgcc gttctcgacc tcgaccgtcc cgtcgcggtg gatccgcacg
      721 aggacctgcg agcccggcgc gacgaacgtg taccagaggg aggcggcgca gccgatcccc
      781 cgccggagcg ggctgccgga ggctgcagcg gcctcgttcg ccttcgtgat ttccgcgcgg
      841 cgcgaccagc cgaactcctt cgcgccgatt tcccattcgg cgcggcgcac ctcggacgga
      901 tcgttcttga gccggagcgc gagcgggtcc atgccgaggc ggctcgcgag ctcctcgagc
      961 gccgtctcga ccgcgaagca tccctgggga tggcccggag cgcgcatcgc gcatccgggg
     1021 ccgaggtgcg tgaacacgtc ggactccgcg gccctcacgg cggggaagcc gtacgcggcc
     1081 ttcacgaatc cggccgtccc ggtcccggtt gcgacgccgg cggtgccgaa cgactcgtag
     1141 tcggccgccg tgacggtccc gtccttccgg tagccgagct tcacgcggtt ccacgagtcg
     1201 ggccggttgc cggacgagag ctggtcctcc ttgcgttcaa gcgccatgag gaccggtctg
     1261 ccggccttct tcgcgagctt cgcggtgaac acgccgtagt cgcgcgcgcc gaacttcgag
     1321 ccgaagccgc cgcccgtgaa ctcggcgcgg acgatcacct tgtccttcga gagcttgaag
     1381 acctccgcga gctcgtcgcg gacgctgaac gtgccctgcg tggaggcctg gacctccagc
     1441 gtgtcgccga cccattccgc gaacatggag tgcgtctcca tgcaggtgtg cgtctggacg
     1501 gacgtcgtcg tgacgatctc gagggtcccc tcggcctcgg cgaagccctt ctcgacgtcg
     1561 cccttgccgg agggcttcgg cccccggacg ttgcctttct gcgggagcgc ggccgcggcg
     1621 ccgggctcgt cgcccgcggt gcgtttctcc tcgaccgact tctcgaagac gagggtcgcg
     1681 ccctccttcc gggccgcggc gaggctgacg gtgtggggga ggacgtcgta cgtgaccttg
     1741 acggcgcgca tggcgtcctc ggcggcggcg cgcgtcggag ccgcgagcgc gacgacggcc
     1801 tcgccgtggt agcgcacgac cttcttcccg gggttctcga tcacgactcc gggcatcttc
     1861 tcggcccgag agacgtcgat ggacacgatc ttcgcgtgcg cgtgggggga gcggacgatg
     1921 cgcgcgtgga gcatcccggc cttgcgcgcg tcgtgcgtgt agaccgcgcg gcccgtgact
     1981 ttctcgggtc cgtcgaggcg gcgtcgccgc cttcccgtga ccgggcgctc tccgacgcga
     2041 tagggagctg gatcccccgg aggaagagag attttcttag aaagggtatg gccagccagg
     2101 cccacttcga ccagcgcctc gcctgcggct acctctggca ccttctcaga aatcttcttc
     2161 ttcttcttct ctttctcgtt ctctgttttc t
//
LOCUS       DAOMFY010000436         2188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_24946_length_2188_cov_7.766057, whole genome shotgun sequence.
ACCESSION   DAOMFY010000436 DAOMFY010000000
VERSION     DAOMFY010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24946_length_2188_cov_7.766057"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLB01_19675"
     CDS             complement(<1..229)
                     /locus_tag="PLB01_19675"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HQQ79574.1"
                     /translation="MRARPRSGRGPGGRRGDALRLSALAAEVPHAAAGPGDPDVTSIT
                     HDSRAAKPGSLFAAYPGLRSDGRRFVADAVAR"
     gene            complement(172..>2188)
                     /locus_tag="PLB01_19680"
     CDS             complement(172..>2188)
                     /locus_tag="PLB01_19680"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HQQ79575.1"
                     /translation="WVLLGLVGLWILGLLVRLVDLQVVRQHEFARRAKKQQERVTELA
                     TRRGTIEDADGKPLAVNARADSVFATPSDVEDPAGEAALLAPILKMPAKDVQRRLTAE
                     KEFVWIARRIPNGAADRVRALSAEKRLPGVSLVPESTRRYPEGPLAAAVLGYVGTDDH
                     GLGGLEFRYDSEVRGRPVTVTLLKDAARRSYAARTRAGGEAPAEGIEGASLRLTLRSA
                     IQHAAERELQRVAAEFHPKGASAIVLDPATGAVLALASWPPFDPNRFGESDAEARKCR
                     PLTDAYEPGSTFKIVAAGAALDGGTIGLDDAVDCGGGVLSIGGTTIHEHGGKGWSALT
                     IGDILAHSSNIGIAHVALGLGRGPFYKAMRSFGFGQKTGIELEGETAGLLKDPAQWSA
                     LSLPTMAFGQEVGVTVLQMARAYAAIANGGVLPSLHVVDGVKRSGSAEFRSIPRPEPP
                     RVLTEGTARQMRRLLARVVEMGTGKAAAIPGYTAAGKTGTAQKAIPGAGYSKEKYVAS
                     FIGFVPADAPRVVIAVVVDEPRGKTYGGDVAAPVFSQIGAEVMRVLKVAPQRPDGARS
                     PILTADLSAGAANAYAGAQLAAAGVVPAVNRTGSEVDEDAVPDLSGKSARDAVRALAS
                     RGVAARLRGSGFVVSQDPPPGAPLSRGTTCALVLAPAGGRAAEGGMP"
BASE COUNT          292 a          799 c          801 g          296 t
ORIGIN      
        1 cgcgcgcgac ggcgtcggcg acgaaccgtc ggccgtcgga ccgcaggccc gggtacgcgg
       61 cgaagagcga gcctggcttc gcggcgcgcg agtcgtgcgt gatggacgtg acgtccggat
      121 ccccggggcc tgcggcggcg tgcggaacct cggcggcgag cgcggagagc ctcaaggcat
      181 ccccccttcg gccgcccggc ccccggccgg agcgaggacg agcgcgcacg tcgtcccgcg
      241 ggaaagcggc gcgcccggag gcgggtcctg cgagacgacg aagcccgagc cccggagccg
      301 cgcggcgacg ccgcgcgaag cgagcgcacg caccgcgtca cgcgcgctct tgcccgaaag
      361 gtccggaacg gcgtcctcgt cgacctcgga tccggtgcgg ttgacggcgg ggacgacgcc
      421 cgcggcggcg agttgcgcgc ccgcgtacgc gtttgcggcg cctgccgaga gatccgcggt
      481 gaggatcggc gagcgcgcgc cgtccggccg ctgcggcgcc accttgagga cgcgcatgac
      541 ctcggcgccg atctgcgaga agacgggcgc cgcgacgtcg ccgccatagg tcttcccgcg
      601 cggctcgtcc acgacgaccg cgatcacgac gcgcggcgcg tccgccggca cgaagccgat
      661 gaacgacgcc acgtacttct ccttcgagta cccggcgccg gggatcgcct tctgcgcggt
      721 tcccgtcttg ccggcggcgg tgtaacccgg aatggcggct gcttttcccg ttcccatctc
      781 gacgaccctc gcgaggagcc gtcgcatctg tcgtgccgtc ccctccgtga ggacgcgggg
      841 cggctcgggg cgcgggatgc tccggaactc cgcagatccg ctccgtttca cgccgtcgac
      901 gacgtgcagc gaggggagga cgcccccgtt cgcgatggcg gcgtaggcgc gcgccatctg
      961 gaggacggtc acgccgacct cctgcccgaa cgccatcgtc gggagggaga gcgcgctcca
     1021 ctgcgccggg tccttgagga ggcccgccgt ctcgccctcg agctcgatgc ccgtcttctg
     1081 gccgaacccg aagctgcgca tcgccttgta gaacggtccg cgcccgaggc cgagcgcgac
     1141 gtgtgcgatc ccgatgttcg aggagtgcgc gaggatgtca ccgatcgtga gcgcactcca
     1201 gcccttgccg ccgtgctcgt ggatcgtcgt ccccccgatc gacagaacgc cgcctccaca
     1261 atccacggcg tcgtcgaggc cgatcgtccc gccgtcgagc gccgcgcccg ccgccacgat
     1321 cttgaacgtc gagccgggtt cgtacgcatc cgtcagggga cggcacttgc gcgcctcggc
     1381 gtcagactcg ccgaagcggt tcgggtcgaa cggcggccac gacgcgagcg cgagaacggc
     1441 gcccgtcgcc gggtcgagga cgatcgcgga agcgcctttc ggatggaatt cggccgcgac
     1501 gcgctggagc tcgcgctcgg ccgcatgctg gatcgcggag cgcagcgtca ggcggaggga
     1561 cgctccctcg atgccttcgg cgggggcctc gccgccggcg cgcgttcgcg ccgcgtagga
     1621 acgacgcgcc gcgtccttca ggagcgtcac ggtgacgggc cggccgcgca cctccgagtc
     1681 gtaccggaac tcgaggccgc cgaggccgtg gtcatccgtc ccgacgtagc cgaggaccgc
     1741 cgccgcgagc ggcccctcgg gatagcggcg cgtggactcg gggacgagcg agacgcccgg
     1801 gaggcgcttt tcggccgaga gcgcgcgcac gcggtcggcg gcgccgttcg ggatgcgccg
     1861 cgcgatccag acgaattcct tttccgcggt gagtctcctc tgaacgtcct tcgccggcat
     1921 cttgaggatc ggcgcgagca gagccgcttc ccccgcggga tcttcgacgt ccgacggggt
     1981 cgcgaagacc gagtccgcgc gggcgttcac ggcgaggggc tttccgtccg cgtcctcgat
     2041 cgtgccgcgg cgcgtcgcga gctcggtgac gcgctcctgc tgcttcttcg cccggcgggc
     2101 gaactcgtgc tgccgcacga cctgcaggtc cacgaggcgc acgaggagtc cgaggatcca
     2161 cagccccacg agtccgagga ggacccag
//
LOCUS       DAOMFY010000437         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_25088_length_2181_cov_6.455786, whole genome shotgun sequence.
ACCESSION   DAOMFY010000437 DAOMFY010000000
VERSION     DAOMFY010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25088_length_2181_cov_6.455786"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="rho"
                     /locus_tag="PLB01_19685"
     CDS             <1..1230
                     /gene="rho"
                     /locus_tag="PLB01_19685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQQ79576.1"
                     /translation="GPGAPGPGGEGAALSNEPAPEARGRYAIPRVEAPVGAAAVSSQA
                     KVVVKGIVRLHGDHSGMLVSPETFFGERGDPFLPKVLVESEDLEDGLLVEAEAVQRGG
                     KGPMVQRIVAIEGLDPAEYRGKYVQFHKGVSVDPDRRLRMETAPEEVSGRVLDLVAPI
                     GRGQRCLIVAPPKAGKTFLLKTMANAIAKNDPDVVIFMLLVDERPEEVTDMQRSITGE
                     VIASPSDLNAAHHIAVSEACFERAKRLVEIGKDVVIFCDSITRMSRAYNNEQRGSGRI
                     LSGGMDARTMEKPRRFFGGARNHEDGGSLTIIATCLIDTGSRMDDVIFEEFKGTGNSE
                     IILDRGLFERRIFPCINIPASGTRKEEKLYDADELPKIHKLRRALSSVKPQDAMELLL
                     SKLAKTKTNKEFLKSIL"
     gene            complement(1338..>2181)
                     /locus_tag="PLB01_19690"
     CDS             complement(1338..>2181)
                     /locus_tag="PLB01_19690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ79577.1"
                     /translation="AARRAARGSGRSGLTMPYAGVNGRRLYYEVGGEGETVVLVHGSF
                     ANADILEGPATAISSGFRAVRYDRRGHGRSGDAPDPLPLAEEAAELAAFLDWFSTERA
                     HLLAHDDGAEIALQFALDFPDRALSLALLAPTLEGFSWRDETRAARRDLLAAYVRSPQ
                     QAIADKWLPSHVFDVARERETPFERLESIYANAHVAGGSLERPPHAGPVQAQRLGEIR
                     ARTAVLVGNREDAERLRCAAAIVQGVPGAEGMTFEGLGRFLHMEDARHVMRRLTDFYI
                     PPEE"
BASE COUNT          357 a          735 c          775 g          314 t
ORIGIN      
        1 ggcccgggag cccccggccc gggaggcgag ggcgctgccc tgtcgaacga gcccgctccc
       61 gaagctcgtg gccggtacgc gatcccccgg gtggaggcgc cggtcggggc cgccgcggtc
      121 agcagccagg cgaaggtcgt cgtcaagggg atcgtccggc tccacggcga ccactcgggc
      181 atgctcgtgt ccccggagac gttcttcggc gagcgtggcg acccgttcct tccgaaagtc
      241 ctcgtcgaat ccgaggacct cgaggacggt ctgctcgtcg aggccgaggc ggttcaaagg
      301 ggcggcaagg gccccatggt ccagaggatc gtcgcgatcg agggcctcga tcccgcggaa
      361 taccgcggca agtacgtcca gttccacaag ggtgtctcgg tcgaccccga ccggcgcctg
      421 cgcatggaga cggctccgga agaggtctcg ggccgcgtcc tcgacctcgt cgccccgatc
      481 gggcgcgggc agcgctgcct gatcgtcgcg ccgccgaagg ccggcaagac cttcctcctc
      541 aagacgatgg cgaacgccat cgcgaagaac gaccccgacg tcgtcatctt catgcttctc
      601 gtggacgagc ggcccgagga agtgaccgac atgcagcggt ccatcacggg cgaggtcatc
      661 gcgtcgccga gcgacctgaa cgcggcgcac cacatcgccg tgtccgaggc ctgcttcgag
      721 agggccaagc gcctcgtcga gatcggcaag gacgtcgtga tcttctgcga ctcgatcacc
      781 cgcatgagcc gcgcctacaa caacgagcag cgcggcagcg gccgcatcct gtcgggcggc
      841 atggacgcgc ggacgatgga gaagccccgg cgcttcttcg gcggcgcgcg caaccacgag
      901 gacggcggct ccctcacgat catcgcgacg tgcctcatcg acacgggcag ccggatggac
      961 gacgtcatct tcgaggagtt caagggcacc gggaacagcg agatcatcct cgaccgcgga
     1021 ctcttcgagc gccggatctt cccgtgcatc aacatccccg cgtccggaac gcgcaaggaa
     1081 gagaagctct acgacgccga cgagctgccg aagatccaca agctccggcg cgcgctctcg
     1141 tccgtcaagc cccaggacgc gatggagctg ctgctctcca agctcgcgaa gacgaagacg
     1201 aacaaggagt tcctcaagag cattctttga ggaagagaaa gaagggcgag agacgagggt
     1261 ctcttagaag ggtgtgtggg gcccgcgctt tcgcccctcg taccttctaa gagaatcctc
     1321 tcttcttttt cttattctta ctcttcgggc gggatgtaga agtccgtcag ccggcgcatg
     1381 acgtggcgcg cgtcctccat gtggaggaag cgcccgaggc cctcgaacgt catcccctcg
     1441 gcgccgggga cgccctggac gatcgcggcc gcgcagcgga ggcgttcggc gtcctcgcgg
     1501 tttccgacga ggacggccgt gcgggcgcgg atctcgccca gccgctgggc ctggacgggg
     1561 cccgcgtgcg gcggacgctc gagcgagccg ccggcgacgt gcgcgttcgc gtagatggac
     1621 tcgaggcgct cgaacggggt ctcgcgctcg cgtgcgacgt cgaagacgtg ggaaggcagc
     1681 catttatccg cgatcgcctg ctgcgggctc cggacgtagg ccgccaggag gtcgcgccgc
     1741 gccgcgcgcg tctcgtcccg ccaggagaat ccctcgagcg tcggcgcgag gagggcgagc
     1801 gacagggcgc gatccgggaa gtcgagcgcg aactggagcg cgatttcggc gccgtcgtcg
     1861 tgggcgagga ggtgggccct ttcggtcgag aaccagtcga ggaatgcggc gagctccgca
     1921 gcctcctcgg cgagcggcag agggtcgggc gcgtctccgg agcggccgtg gccccggcgg
     1981 tcgtagcgga ccgcgcggaa gccggaggag atcgcggtcg cggggccttc gaggatgtcg
     2041 gcgttcgcga acgaaccgtg cacgaggacg accgtctcgc cctcgccgcc gacctcgtaa
     2101 tagaggcggc gcccgttcac gcccgcgtac ggcatcgtca ggccggagcg gccgcttccg
     2161 cgcgcggcgc gacgcgccgc c
//
LOCUS       DAOMFY010000438         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_25114_length_2180_cov_7.674353, whole genome shotgun sequence.
ACCESSION   DAOMFY010000438 DAOMFY010000000
VERSION     DAOMFY010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25114_length_2180_cov_7.674353"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLB01_19695"
     CDS             complement(<1..988)
                     /locus_tag="PLB01_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79578.1"
                     /translation="MRRMRTRLSLAAAVCFVLSLPARAQVAPTLSGETGLFEITTADM
                     LAAGRFSLGLSWSMWPRTAAPVPGSPTYPDDPLRYDLQRIGGSIGYGLTNRWEIVIGT
                     GGNRYNADSYNWHGTINGRALYGSFTHSEMDKVRIGTKILLNPRDPVRATIFGGIAIP
                     TQSENDVNAIGTNRADYDFGASFTYGWVTFQTSYILKGSIGSGTPSPGGFPSGYTLSN
                     EWRNAVGIAVPVVPKVFKAIGEINRVHYDGGTTQASDYSEALLGGRVWFGDFSASGAV
                     RIAIDQWSKYGSSPSPIGGLVQIAYAPPVLQPAKSKIVLPEEPPPPAEPVPPP"
     gene            complement(1135..2046)
                     /locus_tag="PLB01_19700"
     CDS             complement(1135..2046)
                     /locus_tag="PLB01_19700"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HQQ79579.1"
                     /translation="MSFQGSLNELPLADIVQLVAVSGKTGMFSMTRASEKGYVYLQNG
                     QITHAKVGDVEGDDAIYALALWNQGSFQFSPGVESDAHTITRSNTNLLLEAARRSDEW
                     KILSRKIPATDSVPVLISRDGMTGPVTLTPLEWRVVTKTDGQRSIEDIARGLKLSSFD
                     VAKTLYGLVTAELVEISKARAGGAPAPVRALSTVAAPAMPAATGQSVATVSTQSGAFQ
                     ALSKGDEKRSLQILCTKVKQEAEGAAAAPGDLSIERPYRAALAELDRGRGLDVVRDLI
                     RTLEEAIGALRGPAAARGFREKMNPLL"
BASE COUNT          321 a          715 c          795 g          349 t
ORIGIN      
        1 ccggcggggg caccggctcg gccggcggcg gaggctcttc gggcaggacg atcttggact
       61 tcgcgggctg caggaccggc ggcgcgtaag cgatctggac gaggccgccg atggggctcg
      121 gcgacgaccc gtacttggac cactggtcga tcgcgatgcg gaccgcgccg gaggcggaga
      181 agtcgccgaa ccagacgcgc ccgccgagga gcgcctcgga gtagtcggag gcctgggtcg
      241 ttccgccgtc gtagtggacc cggttgatct cgccgatggc cttgaagacc ttcggcacga
      301 cggggaccgc gatgccgacg gcgttccgcc actcgttgga gagggtgtag ccgctcggga
      361 agccgccggg cgagggcgtt ccgctcccga tgcttccctt gaggatgtag ctcgtctgga
      421 acgtcaccca gccgtacgtg aacgaggccc cgaaatcgta gtcggcgcga ttcgtcccga
      481 tggcgttcac gtcgttctcg gactgcgtgg ggatcgcgat cccgccgaag atcgtggcgc
      541 gcaccggatc gcgcgggttg aggaggatct tcgtcccgat ccggaccttg tccatctcgg
      601 agtgggtgaa gctcccgtac agggcccggc cgttgatcgt gccgtgccag ttgtacgagt
      661 cggcgttgta ccggttgccg ccggtcccga tcacgatctc ccagcggttc gtgaggccgt
      721 atccgatgct gccgccgatg cgctggaggt cgtagcggag ggggtcgtcc gggtacgtcg
      781 gcgagccggg gaccggcgcc gccgtccggg gccacatgct ccacgacagg cccagggaga
      841 atctgccggc cgcgagcatg tccgcggtcg tgatctcgaa caggcccgtt tcgcccgaaa
      901 gggtcggggc gacctgcgcc cgcgccggaa gggagagcac gaaacacacg gccgcggcga
      961 gggacagtcg ggtccgcatc cgtctcatcg aatctccttt gccgcctgca tcgggcgtgc
     1021 gtgcccgggt ttggcgggga cttgcctcga accgatgccc agaaaacggg aaattgccga
     1081 ttgagtctaa cccgtcccgg ccttcgagag caaacgaggg gaaggactta agggtcagag
     1141 gaggggattc atcttctcgc ggaacccgcg ggccgccgcc gggccgcgca gcgcgccgat
     1201 cgcctcctcg agcgtccgga tcaggtcgcg cacgacgtcg aggcctcgtc cgcggtccag
     1261 ttccgcgagc gccgcgcggt acgggcgctc gatggacagg tcccccgggg ccgccgccgc
     1321 gccctcggcc tcctgtttca ccttcgtgca gaggatctgg agactgcgct tctcgtcgcc
     1381 cttcgagagc gcctggaacg cgccgctttg cgtcgagacg gtggcgacgc tctggccggt
     1441 ggccgccggc atggccggcg ccgcgaccgt ggagagcgcg cgcaccgggg ccggggcgcc
     1501 gccggctcgc gccttcgaga tctcgacgag ctcggccgtc acgaggccgt aaagggtctt
     1561 tgcgacgtcg aaggacgaga gcttcaggcc gcgggcaatg tcctcgatcg agcgctggcc
     1621 gtccgtcttc gtcacgaccc gccactcgag gggcgtcagc gtgacggggc ccgtcatgcc
     1681 gtcccgggag atgaggacgg gcacggagtc ggtcgccggg atcttgcggg acaggatttt
     1741 ccattcgtcg ctgcggcggg cggcctcgag cagcaggttc gtgttcgagc gcgtgatcgt
     1801 gtgcgcgtcg ctctcgacgc ccggggagaa ctggaacgag ccctggttcc agagcgcgag
     1861 cgcgtagatc gcgtcgtccc cctcgacgtc gccgaccttc gcgtgcgtga tctgcccgtt
     1921 ctggaggtag acgtatcctt tctcggacgc acgcgtcatc gagaacatgc ccgtcttgcc
     1981 ggagaccgcg accagctgga cgatgtcggc gagaggcagc tcgttcagag aaccttgaaa
     2041 agacatcagg tgccatccgg acgatagcag ccggggacgg tcttcctcaa cacgcgtcag
     2101 cccgccgggc gccggacgac gacgcgtgcc gcggcggtga gcgcgtccgg accgcgcgtg
     2161 gcgcctcgac gggcgacgac
//
LOCUS       DAOMFY010000439         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_25207_length_2176_cov_5.960868, whole genome shotgun sequence.
ACCESSION   DAOMFY010000439 DAOMFY010000000
VERSION     DAOMFY010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25207_length_2176_cov_5.960868"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PLB01_19705"
     CDS             complement(<1..1410)
                     /locus_tag="PLB01_19705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79580.1"
                     /translation="MQPEKIGRYVILRTLGRGAMGVVYLARDPQIERELALKTIRFDD
                     AEKSFSQDEAKARFLKEAKISGRLQHPHIVTVFDVGEDQGVLYLAMELVQGGSFSQRL
                     ADPAGFSIRDRVRVVAEVAEALAHAHERGVLHRDIKPANILLSPSLSAKVTDFGIGKL
                     LTGDTELTSTGQMVGSPAYMSPEQIKGDKLDARTDIFSLGVVLYQALTLRKPFPADTL
                     TTLVYQILHEEPADPAAVAGDLPEGLGAIVSRCLAKDRANRYADAAELADDLRAVLGF
                     SPVGSTANFSDSRVKRVRSTPSSPASSPPPSTPPSSPPASPSPNASMAPTAAMPVVSP
                     GPVGSAPALPDSESPTITTGRRMGEVAARAEGRVPPPAAKKRVSPATLGALGIVAVLA
                     VLVAVALMKGGAREVQPGSEPPPVPTAAPSPSPALTAPPAALPAPSTGGPAVAVVETP
                     VASATPAAPIQFAPGTRLRE"
     gene            complement(1459..1752)
                     /locus_tag="PLB01_19710"
     CDS             complement(1459..1752)
                     /locus_tag="PLB01_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79581.1"
                     /translation="MDGTKDEERWEGVTSAANESEAALIAGFLESRGIPARVVDRSFH
                     LTPTESEDLSGVEVGVPASRVAEAREELARREKAYDEAPPGSELIGDDEPSGG"
     gene            2046..2176
                     /locus_tag="PLB01_19715"
                     /pseudo
     CDS             2046..2176
                     /locus_tag="PLB01_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907796.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
BASE COUNT          307 a          776 c          807 g          286 t
ORIGIN      
        1 ctcgcgcagg cgggtgccgg gcgcgaactg gatgggggcc gccggagtgg cgctcgcaac
       61 cggagtctcg acgaccgcga cggcgggacc gcccgtggac ggagcgggca gggcggcggg
      121 cggcgccgtc aaggccggcg aaggcgaggg ggcggccgtg gggaccggcg gcggctcgga
      181 ccccggctgt acctccctgg ccccgccctt catgagcgcg acggcgacga ggacggcgag
      241 gacggccacg attcccaggg cgcccagagt cgccggggag acgcgcttct tcgcggcagg
      301 cgggggaacg cgtccctcgg ctctcgccgc gacctcgccc atccggcggc cggtcgtgat
      361 cgtcggggac tcggagtccg gcaatgccgg cgcggacccc acgggccccg gagacacgac
      421 cggcatggcc gcggtgggcg ccatggacgc gttcggcgac ggcgacgccg gcggtgacga
      481 aggcggcgtc gacggcggcg gggacgaggc gggggacgac ggcgtcgacc ggacgcgctt
      541 cacgcggctg tccgagaagt tcgccgtcga gccgaccggc gagaagccga gcacggcgcg
      601 caggtcgtcc gcgagctcgg cggcgtccgc gtagcggttc gcgcggtcct tcgcgagaca
      661 gcggctcacg atcgccccca ggccctcggg caggtcgccc gccacggcgg ccggatcggc
      721 gggctcctcg tggaggatct ggtagacgag cgtggtgagg gtgtcggcgg ggaacggctt
      781 tcgcagcgtc agggcctggt agaggacgac tccgagcgag aagatgtccg tccgcgcgtc
      841 cagcttgtcg cccttgatct gctcgggcga catgtaagcc ggcgagccga ccatctgccc
      901 cgtagacgtg agctccgtgt cgcccgtcag gagcttgccg attccgaagt ccgtgacctt
      961 cgccgaaagc gagggcgaga ggaggatgtt cgcgggcttg atgtcgcggt gcaggacgcc
     1021 gcgctcgtgg gcgtgcgcga gcgcctcggc gacctcggcc acgacccgca cgcggtcgcg
     1081 gatggagaag ccggccgggt cggccagtcg ctgcgaaaag cttccgccct gcacgagctc
     1141 catcgcgagg tagagcacgc cctggtcctc gccgacgtcg aagaccgtca cgatgtgcgg
     1201 gtgctgcagg cggcccgaga tcttcgcctc cttcaggaag cgggccttgg cctcgtcctg
     1261 gctgaagctc ttctccgcgt cgtcgaagcg gatcgtcttc agggcgagct cgcgctcgat
     1321 ctgcgggtcc cgcgcgagat agaccacgcc catcgcgccc cgtccgagcg tgcgcaggat
     1381 gacgtaccga ccgatctttt cgggctgcaa tgagactccg gcgccggatt ctagcggacg
     1441 cgcccggcgg tgccgccgtc agccgccgga cggttcgtcg tcaccgatca gttccgatcc
     1501 gggaggcgcc tcgtcgtagg ccttttcgcg gcgcgcgagc tcctcgcgcg cctcggcgac
     1561 gcgcgaggcc gggacgccga cctccacgcc ggacagatcc tcgctctcgg tcggcgtcag
     1621 gtggaaactg cggtccacga cgcgggccgg aattccgcgg ctctcgagga atcccgcgat
     1681 gagcgccgcc tcggactcgt tcgcggccga cgtgacccct tcccagcgct cttcgtcctt
     1741 cgtcccgtcc atttccgcct ccttcaagcg accacgcgga tcgcaggtgc acccgtaccg
     1801 gcctccaaaa accgtcccac gaccgccgcg ggagcgtcac ccgccgcccg gagcgccgcc
     1861 acgagcggct ccgcctccga ctcccggacg gcgaagagca atccccccga cgtctgggcg
     1921 tcggccgcaa ggagaagatc gtcctcggcg acgccggccg caacgtcgag gaagggccgc
     1981 gcggcggcga ggttccgccg cgagccgtcg ggcgcgaagc ccgccgcgac gagacgctgc
     2041 gcgcccgcga gctggcgcac ggcagcctga gcgagctgca catcaccgac gacatgcacg
     2101 cgcgcaagct gaagatggcg acgctggccg acggcttcat cgcgctcccc ggcggctacg
     2161 gcacctggga ggagct
//
LOCUS       DAOMFY010000440         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_25558_length_2159_cov_4.731939, whole genome shotgun sequence.
ACCESSION   DAOMFY010000440 DAOMFY010000000
VERSION     DAOMFY010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25558_length_2159_cov_4.731939"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLB01_19720"
     CDS             complement(<1..703)
                     /locus_tag="PLB01_19720"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ79582.1"
                     /translation="MADVVFVAGRDPRSELDGGHSGYVRAHAKAALAAGFTPHLVCVG
                     PSAEVAATPYGVVRRAASPARPFRQFMIPGHALALRRAVSSAVAETGARVVHGFGVWT
                     WAAAAARDRLEGRGLPLRVLASAYTTLADETLSKIEGASPADGVARKAAFLLEHAAAL
                     ATVRFERRGYRGADLVLVNYESVRRLLEARHGKGLRTRRVPYASESAFDPSRPDAGGL
                     QPAQHAVAPIVEDAGD"
     gene            complement(696..1907)
                     /locus_tag="PLB01_19725"
     CDS             complement(696..1907)
                     /locus_tag="PLB01_19725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQQ79583.1"
                     /translation="MLDETRLDPREMDELGDLVPGPEPELAPGRVVRVCETRLDGNEL
                     KYLTECVTSNWISSAGPFVRRFEDAFAAATGCAHAVSCSSGTAALHLVLAALGLGPGD
                     EVVVPAFSMIATANAVRYTGATPVLADAAPGSCNVSAETIAPRITRRTRALLVVHTYG
                     HPVDMDPIRALADRHGLLVVEDAAEAHGALYRGRPVGSLADAATFSFYGNKIVTTGEG
                     GMVTTQDGNLARVVKRLRDHAFSDERHFWHTYLGYNYRMTNMQAAVGLAQVERMPELV
                     AARRENRRRYDARLAGIPGLTLPSEAPDVASVFWMYGVLVGDAFGLSRDALRAALAAC
                     GVETRTYFVPIHVQPVHRAAYRGESYPVAEDLCRRGLYLPSGPALSDADVDFVAGAIE
                     KARPAPVPALG"
     gene            2020..2159
                     /locus_tag="PLB01_19730"
                     /pseudo
     CDS             2020..2159
                     /locus_tag="PLB01_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
BASE COUNT          300 a          751 c          849 g          259 t
ORIGIN      
        1 cgtcgcctgc gtcttcaacg atcggggcta cggcgtgctg cgcgggttgc aggccgccgg
       61 catccggccg cgacgggtcg aacgcgctct cggacgcgta cgggacgcgc ctcgtgcgca
      121 ggccctttcc gtgccgcgcc tcgaggaggc gccggacgga ctcgtagttg acgagcacga
      181 ggtcggcgcc gcggtacccg cgccgctcga agcgcaccgt cgcgagggcg gccgcgtgct
      241 cgagcaggaa cgcggccttg cgcgcgaccc cgtcggcggg cgaggcgccc tcgatcttcg
      301 agagggtctc gtcggccagc gtcgtgtacg cgctcgcgag cacgcggagc gggaggccgc
      361 gcccctcgag gcggtcgcgc gcggctgcgg ccgcccaggt ccagactccg aatccgtgca
      421 cgacgcgggc gcccgtttcg gcgacggcgg acgacacggc tcgccggagg gcgagcgcgt
      481 ggcccgggat catgaactgg cggaacggcc gcgccggcga ggccgctcgg cggacgacgc
      541 cgtacggcgt cgcggcgacc tcggcggacg ggcccacgca gacgaggtgc ggcgtgaagc
      601 cggcggccag ggcagccttt gcgtgggcgc ggacgtagcc ggaatgcccg ccgtcgagct
      661 cggaccgcgg gtcgcggccc gcgacgaaga cgacgtcagc caagcgcggg cacgggcgcg
      721 gggcgcgcct tctcgatcgc gccggcgacg aaatcgacgt ccgcgtcgga cagagccggg
      781 cccgagggga gatagaggcc gcgccggcag aggtcctcgg cgacggggta gctctcaccg
      841 cggtacgccg cgcggtggac cggctgaacg tggatcggca cgaagtaggt ccgcgtctcg
      901 acgccgcacg ccgcgagcgc ggcccgcagg gcatcgcgcg agaggccgaa cgcgtccccg
      961 acgaggacgc cgtacatcca gaacacgctc gcgacgtccg gcgcctcgga cgggagcgtc
     1021 aggcccggga tgcccgcgag ccgcgcgtcg tagcgccgcc ggttctcgcg gcgcgcggcg
     1081 acgagctccg gcatccgctc gacctgcgcg aggccgacgg cggcctgcat gttcgtcatc
     1141 cggtagttgt agccgaggta cgtgtgccag aagtggcgct cgtccgagaa cgcgtggtcg
     1201 cgcaggcgct tcacgacgcg ggcgagattc ccgtcctgcg tcgtgaccat gccgccctcg
     1261 ccggtcgtga cgatcttgtt tccgtagaag gagaacgtcg ccgcgtccgc gaggctgccg
     1321 acggggcggc cgcggtaaag ggcgccgtgc gcctcggcgg cgtcctcgac gacgagcagc
     1381 ccgtggcggt ccgcgagggc gcggatgggg tccatgtcca cggggtggcc gtacgtgtgg
     1441 acgacgagga gcgcccgcgt ccgcctggtg atgcgcggcg cgatcgtctc ggccgagacg
     1501 ttgcacgagc ccggcgcggc gtcggcgagg accggcgtcg cgcccgtgta gcggacggcg
     1561 ttcgccgtcg cgatcatgct gaacgcgggc acgacgacct cgtcgccggg gccgaggccc
     1621 aacgccgcga ggacgaggtg cagggccgcc gtgccggacg agcacgagac cgcgtgggcg
     1681 caacccgtcg cggcggcgaa cgcgtcctcg aagcggcgca cgaacggtcc ggccgaggag
     1741 atccagttgg acgtcacgca ctccgtgagg tacttcagct cgttgccgtc gaggcgcgtc
     1801 tcgcacaccc ggacgacgcg gcccggcgcg agctccggct ccggccccgg cacgaggtcg
     1861 ccgagctcat ccatctcgcg ggggtcaaga cgcgtttcgt cgagcatcca gttctcctga
     1921 cagcggagtc gtgatccatt ccgcgagggc cgtggagcgg gcgagcgcgt ccgaggagac
     1981 ggacgggctc tcggcggaga aggcgcttcc gtaaatcgcc gaaaggcgcg gagcgaggag
     2041 cgggtgggcg gcgccggcaa cgtggccctc aacatcgccg ccctgggaag ccgcgtcagc
     2101 ctcctcggct atcgcggccg ggacgaggcc ggagcgagcc tggcgggact cctggaacg
//
LOCUS       DAOMFY010000441         2136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_26063_length_2136_cov_5.355118, whole genome shotgun sequence.
ACCESSION   DAOMFY010000441 DAOMFY010000000
VERSION     DAOMFY010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26063_length_2136_cov_5.355118"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLB01_19735"
     CDS             <1..871
                     /locus_tag="PLB01_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79584.1"
                     /translation="QTATQEIVDAPRDDRFVRPIYALFWPAFLLVLFDAAAARAGRLA
                     AGTAALAAACAPYLAFSLHGGAAGTYSDLPLACFWGAGLLLLTAEGAGRGEGLASGLL
                     LGAAVLTKNEGLPLAAVALAGALAWAVAGRRLAGSRYSLLAAAGIAAAAALLLFSWRS
                     GIPPRYDEGQVAAFGFAALAKGAVTRLPEAARACFHAMADRKEWGFTWLVVLLGLVLG
                     ARRLGRLATVPLAVSLAGALAFYAAAYSLSVWPADELARTTWDRFLVQMGLPLFALLA
                     LALDPRPEPPQRT"
     gene            complement(859..>2136)
                     /locus_tag="PLB01_19740"
     CDS             complement(859..>2136)
                     /locus_tag="PLB01_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ79585.1"
                     /translation="DAGFQTAVRMPTSGVPGVFATLDAGAPRTVGLYFMYDVKQADPA
                     EWKVAPPFEAKIVDKPGFGKALVGRGSVNQKGPEAAFLAALHAFRGAGVKLPVNLVLV
                     AEGEEEIGSPHIPEIVRKPEVLAALKNTTGVFMPGAGQDPDGSVQVSLGAKGVVELEL
                     VSSGAKWGRGPSKDVHSANRARLDSPAFHLVQALATLVNADGDPAIDGLTAAVKPPNA
                     AQTAMIDAAAKRLDEATAKKMLGAARWAHDDSWRASLERLAFSPTVNIQGLVGGYMGP
                     GGKTMLPNKALAKLDLRLVPDMTFDGAIAALKAHLAKRGFGDIEVNVSGGYGPTETSA
                     DAPLIRAQMAVYKRAGLDPMLWVRNAGSYPGYVFTGEPLKLASGHHGLGHGSGAHAPD
                     EYFVIESTNPKVAGFDGAARSFVDYLYELAQVR"
BASE COUNT          239 a          766 c          788 g          343 t
ORIGIN      
        1 gcagacagcg acgcaggaga tcgtcgacgc gccgcgggac gaccgcttcg tgcgtccgat
       61 ctacgcgctc ttctggccgg cgttcctgct cgtcctcttc gacgcggccg ccgcgcgcgc
      121 cggccgcctc gcggcaggta ccgccgcgct cgccgcggcg tgtgcgccgt acctcgcgtt
      181 ctcgctgcac ggcggggcgg cgggaacgta cagcgacctt ccgctcgcgt gtttctgggg
      241 agcgggactg ctgctcctga ccgccgaggg ggcgggccgc ggcgagggcc tggcctcggg
      301 tctcctgctg ggagcggccg tgctcacgaa gaacgagggc ctgccgctgg cggcggtcgc
      361 gcttgccggc gcgctcgcgt gggccgtcgc gggacggcgg ctcgccggct ctcgatactc
      421 actcctggcc gctgccggta tcgccgctgc ggccgctctt cttctcttct cgtggcggtc
      481 cgggatcccg ccgcgctacg acgagggcca ggttgcggcc tttgggttcg ccgcgcttgc
      541 gaagggcgcg gtcacgcgcc tcccggaggc ggcccgtgcc tgcttccacg cgatggcgga
      601 tcgcaaggag tggggattca cgtggctcgt cgtgctgctc gggctcgtcc tcggcgcgcg
      661 gcgtctcggc cggctcgcga cggtcccgct cgccgtctcg ctcgcgggcg ctctcgcgtt
      721 ctacgcggcg gcgtactccc tctccgtgtg gccagccgac gagctcgccc ggacgacgtg
      781 ggaccggttc ctcgtccaga tggggctgcc gctcttcgcg ctcctcgcgc tcgccctcga
      841 cccacggccg gagccgcctc agcggacctg agccagctcg tagaggtagt cgacgaacga
      901 gcgcgcggcg ccgtcgaagc ccgcgacctt cgggttcgtc gactcgatca cgaagtactc
      961 gtcgggcgcg tgcgcgccgc tgccgtgccc gagtccgtgg tggccggagg cgagcttgag
     1021 cggctcgccc gtgaaaacgt agcccgggta cgagcccgcg ttcctcaccc agagcatcgg
     1081 gtcgaggccc gcgcgcttgt agacggccat ctgcgcgcgg atgagcggcg cgtcggcgga
     1141 cgtctccgtg gggccgtagc cgcccgagac gttcacctcg atgtcgccga agcctctctt
     1201 cgcgaggtgg gccttcaggg ccgcgatcgc gccgtcgaac gtcatgtcgg ggacgaggcg
     1261 gaggtcgagc ttcgcgagcg ccttgttcgg gagcatcgtc ttgccgccgg ggcccatgta
     1321 gccgccgacg aggccctgga tgttcacggt cggcgagaac gcgaggcgct cgagcgacgc
     1381 gcgccaggag tcgtcgtgcg cccagcgcgc cgcgccgagc attttcttcg cggtggcttc
     1441 gtcgagacgc ttcgcggccg cgtcgatcat cgcggtctgg gcggcgttcg gcggcttgac
     1501 ggcggcggtc agcccgtcga tcgcgggatc gccgtctgcg ttcacgagag tcgcgagcgc
     1561 ctgcacgagg tggaacgcgg ggctgtcgag gcgcgcgcgg ttcgcggagt ggacgtcctt
     1621 cgacgggccg cggccccact tcgcgccgct cgagacgagc tccagctcga cgacgccctt
     1681 ggcgccgagc gagacctgca cggagccgtc ggggtcctgc cccgctccgg gcatgaagac
     1741 gcccgtcgtg ttcttcagcg ccgcgaggac ttcgggcttc cggacgatct cggggatgtg
     1801 cggcgagccg atctcctcct cgccctcggc gacgaggacg aggttgacgg gaagcttcac
     1861 gcccgcgccc cggaacgcgt ggagcgccgc gaggaacgcg gcctccgggc ccttctggtt
     1921 cacggagccg cggccgacga gagccttgcc gaagcccggc ttgtcgacga tcttcgcctc
     1981 gaagggcggc gcgaccttcc actcggcggg atccgcctgc ttgacgtcgt acatgaagta
     2041 gaggccgacg gtcctcggcg cgcccgcgtc gagcgtcgcg aacaccccgg ggacgccgga
     2101 cgtcggcatc cggacggcgg tctggaaccc cgcgtc
//
LOCUS       DAOMFY010000442         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_26213_length_2129_cov_7.013983, whole genome shotgun sequence.
ACCESSION   DAOMFY010000442 DAOMFY010000000
VERSION     DAOMFY010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26213_length_2129_cov_7.013983"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLB01_19745"
     CDS             <1..840
                     /locus_tag="PLB01_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79586.1"
                     /translation="VTKPFVSQGLAALVRDLVRRAAEAKASAPPEPPPAPEPPPPPPP
                     PAPAPVEAGESTDPTVADAGSFYTAPLAVPELMEAPPHPPLPPPAPLSDDVFSTTAIP
                     VFSELPPPERSDFEPPTGPIPPVPPPPPSLGPDRGYEMNLDSLDDTPEPQRRDLDEDI
                     AAFERSDKGKTRRPEAWEKLGGEDGAGSGASPESRSDLEALAAEASLTDLKSLIPDSV
                     PAAPVAASPAAALSDADVDRIARRVLELGGERVVRRIAWDVVPELAERLVKERLEELE
                     RAD"
     gene            872..>2129
                     /locus_tag="PLB01_19750"
     CDS             872..>2129
                     /locus_tag="PLB01_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQQ79587.1"
                     /translation="MKELEKTFDPSHFEMRWYKAQEEAGFFSPEAAAPGAVPFTIVIP
                     PPNVTGRLHVGHALGRTLEDVLCRWRRMQGRAVLWVPGVDHAGIATQMVVERDLKERE
                     GKTRHDLGREAFLAICREWAGSRRGDILNQIRRLGASCDFTREYYTLDETRSKAVRYV
                     FTTLYKQGLAYRAERMVNWCPSCATVLSDLEVNHVETSGHLWSIAYPVEGGGEVVVAT
                     TRPETMLGDTAVAVHPDDPRYTALVGKRVTLPLTGRTIPVIADPFVDPKFGTGVVKVT
                     PAHDPNDFEAGRRHGLDLVTVIGFDGKMTKAAGDAYAGLDRSEARKKVLRDLDAQGRR
                     RGEKSHLVPLARCQRCDTVVEPLVSIQWFVKIGPLAEKAVAATDSGETAFTPDIWKKT
                     YDEWMKNIRDWCVSRQLWWGHEIPAWY"
BASE COUNT          312 a          795 c          735 g          287 t
ORIGIN      
        1 gtcacgaagc cgttcgtctc gcagggactc gccgcgctcg tgagggatct cgtccgccgc
       61 gcggccgagg cgaaggcttc cgcgccgccg gagcctccgc cggcgcccga accgcctccg
      121 cccccgccgc ctccggcgcc cgcgccggtc gaggccggag agtccacgga tccgaccgtc
      181 gcggacgcgg gttcgttcta cacggcgccg ctggccgtcc cggagctgat ggaggcgcct
      241 ccgcatccgc ccctccctcc gcccgcgccg ctctccgacg acgtgttttc gacgacggca
      301 attccggtct tctccgagct gccgccgccg gagcgctccg acttcgaacc gccgaccggg
      361 ccgatcccgc ctgtgccgcc ccctcctcct tccctcgggc ccgaccgcgg gtacgagatg
      421 aacctcgatt ccctggacga cactccggag ccgcagcgcc gcgacctcga cgaagacatc
      481 gccgccttcg agcgctccga caagggcaag acacgccgcc cggaggcgtg ggagaagctc
      541 ggcggagagg atggcgccgg gtccggggcg tccccggagt cgcgctccga cctcgaggcc
      601 ctcgccgccg aggcgagcct caccgacctc aagagcctca tccccgattc cgtgccggcc
      661 gcgcccgtcg ccgcgtctcc cgcggcggcg ctttcggacg ccgacgtcga ccgcatcgcg
      721 cgccgcgtgc tcgagctggg cggcgaacgc gtcgtgcgcc gcatcgcctg ggacgtcgtc
      781 ccggagctgg ccgagcgcct cgtcaaggag cgcctcgagg agctcgaaag agcggactga
      841 ggactcggct ttccgatatc ctcggcggcc tgtgaaggaa ctcgaaaaga ccttcgaccc
      901 ctcgcacttc gagatgcgct ggtacaaggc gcaggaagag gcgggcttct tcagtcccga
      961 ggcggccgca cccggcgcgg tcccgttcac gatcgtgatc ccgccgccga acgtcacggg
     1021 gcggctgcac gtcggccacg cgctcgggcg cacgctcgag gacgtcctct gccgctggcg
     1081 ccgcatgcag ggccgcgccg tcctgtgggt gcccggcgtc gaccacgcgg gcatcgcgac
     1141 gcagatggtc gtcgagcgcg acctgaagga gcgggagggc aagacgcgcc acgacctcgg
     1201 ccgcgaggcg ttcctcgcga tctgccgcga gtgggcgggg tcgcggcgcg gcgacatcct
     1261 gaaccagatc cggcgcctcg gcgcctcgtg cgacttcacg cgggagtact acacgctcga
     1321 cgagacgcgc tcgaaggccg tccgctacgt gttcacgacg ctctacaagc agggcctcgc
     1381 gtaccgcgcc gagcggatgg tgaactggtg cccgagctgc gcgacggtgc tcagcgacct
     1441 cgaggtgaac cacgtcgaga cgtcggggca cctgtggtcg atcgcctatc ccgtcgaggg
     1501 cggcggggag gtcgtcgtcg ccacgacgcg ccccgagacg atgctcggcg acacggccgt
     1561 cgccgtgcac ccggacgacc cgcgctacac ggctctcgtc ggcaagcgcg tgacgctgcc
     1621 cctgaccggc cgcacgatcc ccgtgatcgc cgaccccttc gtcgatccca agttcggaac
     1681 aggcgtcgtc aaggtcacgc cggcgcacga ccccaacgac ttcgaggcgg gccggcgcca
     1741 cggcctcgac ctcgtgaccg tgatcggctt cgacgggaaa atgacgaagg ccgcgggcga
     1801 cgcgtacgcg ggcctcgacc ggtccgaggc gcggaagaag gtcctccggg acctcgacgc
     1861 gcagggccgg cgccgcggcg agaagagcca tctcgtcccc ctcgcgcgct gccagcggtg
     1921 cgacacggtc gtcgagcccc tcgtctcgat ccagtggttc gtgaagatcg gcccgctggc
     1981 cgagaaggcc gtcgcggcga ccgattcggg agagacggcg ttcacgcccg acatctggaa
     2041 gaagacctac gacgagtgga tgaagaacat ccgggactgg tgcgtctcgc gccagctctg
     2101 gtgggggcac gagatcccgg cgtggtact
//
LOCUS       DAOMFY010000443         2120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_26433_length_2120_cov_5.292494, whole genome shotgun sequence.
ACCESSION   DAOMFY010000443 DAOMFY010000000
VERSION     DAOMFY010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26433_length_2120_cov_5.292494"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2120)
                     /locus_tag="PLB01_19755"
     CDS             complement(<1..>2120)
                     /locus_tag="PLB01_19755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HQQ79588.1"
                     /translation="CGREGRLATPSRQPAGRNGLLDLDLPRLRLDDPLLRDTNHDHPV
                     AALGAAAWALARRRPFAAPAAERWKTELVAWGTFLFFLAFRAASPEIYSGEKPMDFAF
                     LNALLRSTALPPPEPWLAGSTLSYTYFGHYALAAIGKLLGIHPGVLFNLGIAAVASLT
                     ACGVLAAGAALGGRLRAGAVAVLLAMFAAPPSGVREAVLRWKAGQPLDWHYFWATSRV
                     IAPNGISEYPLWTFLFADLHAHALAMPFTTAFLALLIVLATRDALPTPREEAATIGLL
                     GLLLAAIQITNGWAVPTYACALVFVPLAAYVVARPGGAAASIPGFLRGAALPAAGAAA
                     VAYVLARPFWVHFTPPPRNWGREVGPFASPWDFFNVWAFFLALLVPFAFTALRQGDPP
                     PGRAARAARGILAILLPLSLLSLALKPWHLDQAQSIRGFTALAGLAAAWAAARRGASR
                     EVRLACALAAFGFAVLTGCEVVYVWDRMNTIFKFHLEVWFFFAVAGAAAWRLLRTAGG
                     LLWKSAAAIAFAAVLFTTVTAVPGYLRNGREDWPRATLDGTAYLEVKAPGDRGAYEWI
                     NANVRGVPVVLEAQGDAYQDFSRYSMMTGLPTVLGWEYHTFQRGHSQPEIDRRKADVK
                     AAYTALAPDEVAAVLRRYHVALVAVGPLERKTYGAGILARFRGFPDILTPVYQNPEVT
                     LFAVKGAFAPGAATPPLRVEELPA"
BASE COUNT          336 a          721 c          833 g          230 t
ORIGIN      
        1 cgccggcagt tcctccaccc ggagcggcgg cgtcgcggcg ccgggcgcga acgcgccctt
       61 cacggcgaag agggtcacct cggggttctg gtagacgggc gtcaggatgt ccgggaagcc
      121 gcggaagcgc gcgaggatcc cggccccgta tgtctttctc tcgagcggcc ccacggccac
      181 gagcgcgacg tggtagcgcc ggaggacggc cgcgacctcg tcgggcgcga gcgccgtgta
      241 cgccgccttc acgtcggcct tccggcggtc gatctcgggc tggctgtggc cgcgctggaa
      301 cgtgtggtat tcccagccga ggacggtcgg gagccccgtc atcatcgagt agcgcgagaa
      361 gtcctggtac gcgtcgccct gcgcctcgag gacgacgggg acgccgcgca cgttcgcgtt
      421 gatccactcg taggcgcccc ggtccccggg cgccttcacc tcgagatagg cggtcccgtc
      481 gagcgtcgcc cgcggccagt cctcgcggcc gttgcggagg taaccgggca cggccgtcac
      541 cgtcgtgaac aggaccgccg cgaacgcgat cgcggccgcc gatttccaca gcagcccgcc
      601 ggccgtccga aggagccgcc acgcggccgc gcccgcgacc gcgaagaaga accagacctc
      661 gaggtggaac ttgaagatcg tgttcatccg gtcccacacg tagacgacct cgcatcccgt
      721 gaggaccgcg aacccgaacg cggcgagcgc gcaggcgagg cggacctcgc ggctcgcgcc
      781 tcgcctcgcc gccgcccacg ccgcggcgag gcccgcgagc gccgtgaaac cgcggatgga
      841 ctgcgcctgg tcgaggtgcc agggcttcag cgcgagagag aggagggaca gcgggagcag
      901 gatcgcgagg atcccgcggg cggcgcgcgc ggcgcggccg ggaggcggat cgccctgccg
      961 gagggccgtg aacgcgaacg gcacgaggag cgcgagaaag aacgcccaca cgttgaagaa
     1021 gtcccacggc gaggcgaacg gcccgacctc gcgtccccag ttgcgcggcg gcggcgtgaa
     1081 gtgcacccag aacgggcggg cgaggacgta cgcgacggcc gcggctcccg cggcggggag
     1141 cgcggcgccg cgcaggaagc cggggatcga cgcggcggct ccgccgggac gcgccacgac
     1201 gtacgcggcg agcgggacga agacgagcgc gcaggcgtac gtcgggacgg cccacccgtt
     1261 cgtgatctgg atggcggcga ggaggaggcc gaggaggccg atggtcgcgg cctcctcgcg
     1321 cggcgtcggg agggcgtcgc gcgtcgcgag gacgatgagg agcgcgagga acgcggtcgt
     1381 gaacggcatc gcgagggcgt gcgcgtggag gtcggcgaag aggaaggtcc agagcgggta
     1441 ctcgctgatc ccgttcgggg cgatgacgcg cgacgtggcc cagaagtaat gccagtcgag
     1501 cggctggccg gccttccacc ggaggacggc ctcgcgcacg ccggacggcg gtgccgcgaa
     1561 catcgcgagg agcacggcga ccgctccggc ccggagacgc ccgccgagcg ccgcgccggc
     1621 cgcgaggacg ccgcaggccg tgaggctcgc gacggccgcg atcccgagat tgaagaggac
     1681 gccggggtga atgccgagga gcttgccgat cgcggcgaga gcgtagtgcc cgaagtacgt
     1741 gtaggagagc gtcgagcccg cgagccacgg ctcgggcggc ggcagggcgg tcgaccggag
     1801 gagcgcgttg aggaacgcga agtccatcgg cttctcgccc gagtagatct cggggctcgc
     1861 ggcgcggaac gcgaggaaga agaggaacgt cccccacgcg acgagctccg tcttccaccg
     1921 ctcggcggcc ggcgccgcaa agggccggcg gcgcgcgagt gcccaggcgg ccgcgccgag
     1981 agccgcgacg gggtgatcgt gattcgtatc ccgaagcagc gggtcgtcga gccgcagccg
     2041 cggcagatcc agatccagta aaccgttccg gcccgcgggt tgccgggagg gcgtcgcaag
     2101 gcgcccttcc cgtccgcatt
//
LOCUS       DAOMFY010000444         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_26541_length_2115_cov_5.758738, whole genome shotgun sequence.
ACCESSION   DAOMFY010000444 DAOMFY010000000
VERSION     DAOMFY010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26541_length_2115_cov_5.758738"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..229
                     /gene="yidD"
                     /locus_tag="PLB01_19760"
     CDS             74..229
                     /gene="yidD"
                     /locus_tag="PLB01_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQQ79589.1"
                     /translation="MLPPACRFTPTCSEYARAAVLRHGLSRGLLLAARRLARCHPFHA
                     GGHDPVP"
     gene            252..1973
                     /gene="yidC"
                     /locus_tag="PLB01_19765"
     CDS             252..1973
                     /gene="yidC"
                     /locus_tag="PLB01_19765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQQ79590.1"
                     /translation="METKRLVLAFALSAAVLIGWYVLFPPPAPAPAKPAAPAAAAPRT
                     AAPGTPATASPATAAAPGPAALSPQPVAPAVPVEPIVAAAAETVEVVQPLYTARLSNE
                     GAVLRAFVLAQHRDGAGKPLDLVRQDAPYPGRTLALDPADAFLARAAKARFTVAKEEN
                     GSEKTVRFRYREADGNGLTVTYLFRNAYVLGLKVEREGPGGAAGPVGVVLGPSIGNPS
                     GEELQSRYSTPGGTITVAPGGSVTRRAKDGLKEAVPGLTGLVAAGLEDNYFLTAFLPG
                     AQGVVALRPVSLSAPVPAQTAAPAATPSAAPVPAAESEVVLSGTGALATDVFLGPKAI
                     DILEKTRPGLDRVIDYGWFAVLVKPLLWGLRAIHRYVGNWGVAILLITVVIKVLLYPL
                     THKQLVSMKKMGALQPKVEAIRTKWGSRIKQDPQARVKMNEEMMGLYKQEGVNPAGGC
                     LPLLLQMPILIAFYNLLAHSIELRHAPFMLWITDLSAKDPYYVTPILMTATMWIQQQM
                     TPTTGDATMKRVQNIMPLVMGFLFKDVPSGLVLYWLMQNILTIAQQMLLNRFTDLGHS
                     SMKPKAS"
BASE COUNT          287 a          819 c          705 g          304 t
ORIGIN      
        1 tccagccgcg ccgggcgccg gcgcccgcgc ggcggcggcg gtcttcgccg cgtacaagaa
       61 gctcgtctct cccctgctgc ccccggcctg ccgcttcacc ccgacgtgtt cggagtacgc
      121 gcgggccgcg gttctccgcc acggcctttc ccgcggcctt ctgctcgccg cgcggcggct
      181 ggcgcgctgc catccgttcc acgccggcgg ccacgacccc gtaccctgag aagatcgtcc
      241 aaggagacct cttggagacc aaacgcctcg tcctcgcgtt cgccctttcg gccgccgtcc
      301 tgatcggctg gtacgtgctc ttcccgccgc cggcccccgc gcctgcgaag ccggcggcgc
      361 cggcggcagc cgcacccaga acggcggcgc cgggaactcc cgccacggcc tcccccgcca
      421 cggcggcggc gcccggcccg gccgctctct caccgcagcc cgtcgcgccg gcggttccgg
      481 tcgagccgat cgtcgcggca gccgccgaga ccgtcgaggt cgtccagccg ctctacacgg
      541 cgcgcctctc gaacgagggc gccgtcctcc gcgcgttcgt cctcgcgcag caccgcgacg
      601 gcgcgggcaa gccgctcgac ctcgtgcggc aggacgcgcc gtatcccggg cgcacgctcg
      661 ccctcgaccc ggcggacgcg tttctcgcac gcgcggcgaa ggcgcgcttc accgtcgcga
      721 aggaagagaa cgggagcgag aagaccgtgc ggttccggta ccgcgaggcg gacggcaacg
      781 gactgaccgt gacgtacctg tttcgcaacg cgtacgttct cggcctgaag gtcgagcgcg
      841 agggccccgg cggcgcggcg ggcccggtcg gcgtcgtcct cggcccgagc atcggcaacc
      901 cgtcgggcga ggagttgcag agccgctact cgaccccggg cgggaccatc acggtcgccc
      961 cgggcggctc ggtcacgcgg cgcgccaagg acggcctcaa ggaggccgtg ccgggactca
     1021 cgggcctcgt cgcggccggg ctcgaggaca actacttcct cacggcgttc ctgcccggcg
     1081 cccagggcgt cgtcgcgctc cgccccgtct ccctgagcgc ccccgtcccc gcgcagactg
     1141 cggcgcccgc cgcgaccccg tccgccgcgc ccgtgcccgc ggccgagagc gaggtcgttc
     1201 tctccgggac cggcgcgctc gcgacggacg tcttcctcgg gccgaaggcg atcgacatcc
     1261 tcgagaagac gcggcccggc ctcgaccgcg tgatcgacta cggctggttc gcggtcctcg
     1321 tgaagccgct cctgtggggc ctgcgcgcga tccaccggta cgtcgggaac tggggcgtcg
     1381 cgattctcct catcacggtc gtcatcaagg tcctcctcta cccgctcacg cacaagcagc
     1441 tcgtctcgat gaagaagatg ggcgcgctgc agccgaaggt ggaggcgatc cggacgaagt
     1501 ggggctcgcg catcaagcag gacccgcagg cccgcgtgaa gatgaacgag gagatgatgg
     1561 gcctctataa gcaggagggc gtgaatcccg cgggcggatg cctgcccctc ctcctgcaga
     1621 tgccgatcct catcgcgttc tacaacctcc tcgcgcactc gatcgagctg cggcacgcgc
     1681 cgttcatgct ctggatcacc gacctctccg cgaaggaccc gtactacgtc acgccgatcc
     1741 tcatgacggc gacgatgtgg atccagcagc agatgacgcc gacgacgggc gacgcgacga
     1801 tgaagcgcgt ccagaacatc atgccgctcg tcatgggctt cctgttcaag gacgtgccgt
     1861 cgggcctcgt tctctactgg ctcatgcaga acatcctcac gatcgcccag cagatgctcc
     1921 tcaaccgctt cacggacctc gggcattctt caatgaagcc gaaggcgtcc tgagggcggg
     1981 ccgccgcgga tgtcgccgct cgccggcgac cgtgaacgcg ggggcgacgt gctcgtcgct
     2041 cccgcgacgc cgcccggcgt ctcggccctc gccgtcgtcc ggctgacggg ccccgccggg
     2101 gcggcgtgca ccgtc
//
LOCUS       DAOMFY010000445         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_26590_length_2113_cov_5.868805, whole genome shotgun sequence.
ACCESSION   DAOMFY010000445 DAOMFY010000000
VERSION     DAOMFY010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26590_length_2113_cov_5.868805"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLB01_19770"
     CDS             <1..202
                     /locus_tag="PLB01_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79591.1"
                     /translation="RAAGADAPVTARLVASDPAKDLALLEVPVNGPVLATRLDDDDAL
                     RAAALALGVPPADVRAFVAAHR"
     gene            253..1119
                     /locus_tag="PLB01_19775"
     CDS             253..1119
                     /locus_tag="PLB01_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79592.1"
                     /translation="MSALFAAALLFQVQDLRPATKACPFFVVTTSGERVGALDRPNHD
                     GKPVKFRLCSNGTLTMFAAQDVDWAATDQANAAGNAPAPTPGLAPAPTRASLSGIAKQ
                     TSLRDADAAVKQNQLSGKMKLAGREVSLDDSVPFFGKESVAQHLKLSTFYADATGCPS
                     QRARAYGSVKNISRMKLRSLKALVLIGSIRSGDYNGQVQTMDPSDLVPGEESEIFLWL
                     SCDWAGQASTFRTKNDAVLVMIADVAGRTEEVSKPDGSNPFETPAPAKTAAPSRTPTP
                     ASPYGNAPLKPR"
     gene            complement(1196..1399)
                     /locus_tag="PLB01_19780"
     CDS             complement(1196..1399)
                     /locus_tag="PLB01_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79593.1"
                     /translation="MPIEKVEPGRSSARGTIAPSTLRRRTPTGAISQPPPGRETISAC
                     RASTPAKGSAMSFCWRFEPRRSA"
     gene            complement(1604..>2113)
                     /locus_tag="PLB01_19785"
     CDS             complement(1604..>2113)
                     /locus_tag="PLB01_19785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ79594.1"
                     /translation="ICDGASKTWAMTGWRIGYAANRTLAPVFTRWITNTDSCASQISQ
                     WAAVEAVSGPQQAADAMRARFLERRDFIVGLLNAVPGVSCKTPGGAFYAWPNVTEACR
                     MTGCEDSEAFRRRLLHEAGVAVLADIHFGRRVPGDGQHIRFSYAASNEAIAEGIRRVD
                     AFVRRHARA"
BASE COUNT          291 a          831 c          706 g          285 t
ORIGIN      
        1 gcgggccgcc ggggccgacg cgccggtgac ggcgcgcctc gtcgcttcgg atcccgcgaa
       61 ggacctcgcc ctcctcgagg tccccgtgaa cggcccggtg ctcgcgacac gcctcgacga
      121 cgacgacgcg ctgcgcgcag cggcgctcgc gctcggcgtg cccccggcgg acgtgcgcgc
      181 gttcgtcgcc gcccaccgct gagaagggcc gccgcgggcg cagcccgctt cgaggcgtac
      241 actttcccca cgatgtccgc gctcttcgca gcggcgctcc tcttccaggt tcaggacctg
      301 cggccggcca cgaaggcctg cccgttcttc gtcgtgacga cgtcgggcga gcgcgtcggc
      361 gccctcgacc ggccgaatca cgacggcaag cccgtgaagt tccgcctctg ctcgaacggc
      421 accctcacga tgttcgccgc gcaggacgtc gactgggccg cgaccgacca ggccaacgcc
      481 gcgggaaacg cgcccgcgcc gacgcccggc ctcgcgcccg cccccacgcg tgcctccctc
      541 tcgggcatcg cgaagcagac gtccctccgc gacgccgacg ccgcggtgaa acagaaccag
      601 ctctccggga agatgaagct cgccggccgc gaagtctcgc tcgacgactc ggtgcctttc
      661 ttcggcaagg aatccgtcgc gcagcacctg aagctcagca cgttctacgc ggacgccacg
      721 ggctgtccct cccagagggc ccgcgcctac ggctcggtca agaacatctc gcgcatgaag
      781 ctgcggagcc tcaaggcgct cgtcctgatc ggaagcatcc ggtccggcga ctacaacggc
      841 caggtccaga cgatggaccc gtccgacctc gtgcccggcg aggagtcgga gatcttcctc
      901 tggctctcct gcgactgggc cggccaggcc tcgacgttcc gcacgaagaa cgacgccgtc
      961 ctcgtcatga tcgcggacgt cgcggggcgc accgaggagg tctcgaagcc cgacgggtcg
     1021 aacccgttcg agacccccgc gccggcgaag accgccgccc cctcacgcac gcccacgccg
     1081 gcgtccccct acggaaacgc gccgctcaag ccacgctgat gcccgaggcc ggagcttccc
     1141 tcgccctcat gtggatggac cccgcgacgg gcgagacacg gaaggtgccg ctcgatcacg
     1201 cgctgcgcct cggctcgaac cgccagcaga acgacatcgc gctgcctttc gccggcgtcg
     1261 aagccctgca cgcggagatc gtctcgcgtc ccggcggcgg ctgggagatc gcgcccgtgg
     1321 gcgtcctgcg cctcaaggtg gacggcgcga tggtcccgcg cgccgagctt cgtccgggct
     1381 cgaccttctc gatcggcatc ctcgatttca cggtcggcta cgcgggagcc tcgcccgaac
     1441 gcaccggagc gtccgcccgc cccgccgcga cggtctcggg ctcctcgcgc atcgtcgtgc
     1501 ctccaccccc gcgccgcccc gcggtcgccc tccttgccga gcgcggagcg cacgcgcgcg
     1561 aggatcgcct cgcgcgacgc cgcgttggcg tcgatcgcgc ccgtcatgcg cgcgcgtgcc
     1621 tgcggacgaa cgcgtcgacg cgccggatgc cctcggcgat cgcttcgttc gacgcggcgt
     1681 acgagaaccg gatgtgctgg ccgtcgccgg ggacgcgccg gccgaagtgg atgtccgcca
     1741 gcaccgcgac acccgcctcg tgcaggaggc gcctgcggaa cgcctcggag tcctcgcacc
     1801 cggtcatccg gcacgcctcg gtgacgttgg gccaggcgta gaacgcgccg cccggcgtct
     1861 tgcagctcac gcccggcacc gcgttcagca gcccgacgat gaagtcgcgg cgctcgagga
     1921 accgcgcgcg catcgcgtcc gccgcctgct gcggcccgct caccgcctcg acggcggccc
     1981 actgcgagat ctgcgaggcg caggagtcgg tgttggtgat ccagcgcgtg aacaccggcg
     2041 cgagggtccg gttggcggcg tagccgatgc gccagccggt catcgcccag gtcttggacg
     2101 cgccgtcgca gat
//
LOCUS       DAOMFY010000446         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_26907_length_2100_cov_7.805868, whole genome shotgun sequence.
ACCESSION   DAOMFY010000446 DAOMFY010000000
VERSION     DAOMFY010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26907_length_2100_cov_7.805868"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..602)
                     /gene="ssb"
                     /locus_tag="PLB01_19790"
     CDS             complement(177..602)
                     /gene="ssb"
                     /locus_tag="PLB01_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQQ79595.1"
                     /translation="MAGSVNKVILIGHLGADPEMRATTGGVPIARLRVATTETWNDRS
                     TGQKQEKTEWHTVIAFDKLAGICERYLSKGRLVYIEGSLQTRTWDDKETGQKRYATEI
                     KARDMKMLGGRPDAGSSSGVSAAAPESPEGGDADDDVPF"
     gene            645..>2100
                     /gene="murJ"
                     /locus_tag="PLB01_19795"
     CDS             645..>2100
                     /gene="murJ"
                     /locus_tag="PLB01_19795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQQ79596.1"
                     /translation="MSDTRKLVRSTWGITAWTVVSRLAGVVRDATIARFLGASALSDA
                     FYTALRIPNSIRAVVGEGGLPAAFVPMAKKVERERPGEEGVYAGRMLVLLFCVLVGIV
                     TLGILFAGPLVGLFAGGFRATPGKFELTVLLTRWMFPYVLLVSVAALLEAFLNSKGFF
                     QLSAATPIAWNLAIVAAAWALAPAGVPAVAALTGGVLVGGVLQVALQGPAVRRLGLRF
                     SGSPFGDPEVKRTALQIAPRLYGYGVGQLSLVVSTRTLAAIGDAFVTYNYCAFRLVDF
                     VLGGFVVSLTRTVLPALSDQAFEKDRSAYKATVGFALRLIGFVTIPSMVGLMLLAAPI
                     VDLIFRRGRFGEGDVAKTAFAVVFFAVGLYAAAGVKIVTQAFYALHDTRTPVIVATFD
                     LAVFWALCVWLAKPMQHAGVALATSAGFWINFLVLLALLWRRMGSLGGRAIVFALARL
                     LLASLAMGAVVWVLAHRVLPYDAGWVFLARAGWVA"
BASE COUNT          249 a          736 c          714 g          401 t
ORIGIN      
        1 cgcggaattg atggccaagc cgagagagaa aagaaggaac ggagatttcg gagaagatct
       61 tcttagaagg tgaagagagc tgagagccgc tcgaggggcg tcgaggcctt gcccgacgcc
      121 gcagcgccac acgaccttct aagaaatctt ctcttcctcc gaccttctgc tcttcctcag
      181 aacgggacgt cgtcgtccgc gtcgccgccc tcgggagatt cgggagcggc cgcgctgacg
      241 ccggagctcg agcccgcatc gggccggccg ccgagcatct tcatgtcccg cgccttgatc
      301 tcggtggcgt agcgcttctg gcccgtttcc ttgtcgtccc aggtgcgcgt ctgcagagag
      361 ccctcgatgt agacgaggcg gcccttggac aggtaacgct cgcagatgcc cgcgagcttg
      421 tcgaaggcga tgaccgtgtg ccactcggtt ttttcctgct tctgcccggt ggagcggtcg
      481 ttccacgtct cggtcgtggc gaccctgagg cgcgcgatcg gcacgccgcc cgtcgtggcc
      541 cgcatctcgg ggtcggcgcc gaggtggccg atgaggatga ccttgttgac ggatccagcc
      601 atgaaatctc ctccgatcgg cacggatcct acaatccctc cggcttgagc gacacgcgga
      661 aactggtccg gtcgacctgg ggcatcaccg cgtggacggt ggtgtcccgg ctggcgggcg
      721 tcgtgcggga cgcgaccatc gcccggttcc tcggcgcctc ggccctgtcg gacgccttct
      781 acaccgccct gcgcatcccg aactcgatcc gggccgtcgt gggggaaggc gggctcccgg
      841 ccgcgttcgt cccgatggcc aagaaggtcg agcgcgaacg cccgggcgaa gagggcgtct
      901 acgcgggccg gatgctcgtc ctcctgttct gcgtactggt cggaatcgtg acgctcggca
      961 tactcttcgc cgggccgctc gtcggcctct tcgcgggagg cttcagggcg acgccgggga
     1021 agttcgagct gacggtcctt ctcacgcgct ggatgttccc gtacgtcctc ctcgtctccg
     1081 tggccgctct cctcgaagcc ttcctgaact ccaagggctt cttccagctc tccgcggcga
     1141 ctcccatcgc gtggaacctc gcgatcgtcg cggcggcgtg ggccctcgcg cccgcgggcg
     1201 tgcccgcggt tgcggcgctg acgggcggcg tgctcgtcgg aggcgtcctc caggtcgcgc
     1261 tgcaggggcc ggccgtccgt cgcctcggcc tgaggttctc gggctcgccg ttcggggacc
     1321 ccgaggtcaa gcgcacggcg ctccagatcg cgccgcgcct ctacggctac ggcgtcgggc
     1381 agctttcgct cgtcgtctcg acccggacgc tcgccgcgat cggcgacgcg ttcgtcacgt
     1441 acaactactg cgcgttccgg ctcgtggact tcgtcctcgg cgggttcgtc gtctcgctca
     1501 cgcggacggt cctgcccgcg ctttcggatc aggcgttcga gaaggaccgc tcggcctaca
     1561 aggccaccgt cggattcgcc ctccgcctga tcgggttcgt gacgatcccg tcgatggtgg
     1621 gcctcatgct cctcgccgct cccatcgtgg acctcatctt ccggcgcggc cggttcgggg
     1681 agggtgacgt cgcgaagacg gcgttcgcgg tcgtgttctt cgcggtcggc ctctacgccg
     1741 cggccggcgt caagatcgtc acgcaggcgt tctacgccct ccacgacacg cggacgcccg
     1801 tgatcgtcgc cacgttcgac ctcgcggtgt tctgggcgct gtgcgtgtgg ctcgcgaagc
     1861 ccatgcagca cgcgggcgtc gccctcgcga cgtcggcggg gttctggatc aacttcctcg
     1921 tcctcctcgc cctgctgtgg cggcggatgg gctcgttggg cggccgcgcg atcgtgttcg
     1981 cgctcgcccg cctcctcctc gcgagcctcg cgatgggggc ggtggtctgg gtcctcgccc
     2041 accgcgtcct cccgtacgac gcgggctggg tcttcctcgc gcgcgcgggc tgggtcgcgg
//
LOCUS       DAOMFY010000447         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_27414_length_2079_cov_8.693676, whole genome shotgun sequence.
ACCESSION   DAOMFY010000447 DAOMFY010000000
VERSION     DAOMFY010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27414_length_2079_cov_8.693676"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLB01_19800"
     CDS             <1..540
                     /locus_tag="PLB01_19800"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HQQ79597.1"
                     /translation="ATTHGLDAVAKAHGAPVFETAVGFKYIGELLLQDRIVMGGEESA
                     GFTMEGHVPEKDGPLACLLLAELVATTGKTLGTLLQELFARVGPYYPDRADARLDPKL
                     AETMKKRLAENPENFAGLKVQSIDRTDGQKLLLGDGRWILFRASGTEPVVRIYAESSD
                     KRETNRLLEAAKEYVLRSK"
     gene            651..1922
                     /gene="eno"
                     /locus_tag="PLB01_19805"
     CDS             651..1922
                     /gene="eno"
                     /locus_tag="PLB01_19805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQQ79598.1"
                     /translation="MYAIESIHAREILDSRGNPTVEVECLLEGGASARAGVPSGASTG
                     SREALELRDGDKKRYGGKGVLRAVKNVNETIAPEVLGMDARQQAEIDRLMIELDGTEM
                     KTALGANAILGVSMAVCRAAAEACALPLFRYVGGTGAVTLPVPLMNVVNGGAHADNPL
                     DIQEFMLVPAGFDNFREALRAGSEVFHALKGLLKAKGLATGVGDEGGFAPNLESTRAT
                     LDFLVDAIGKAGYKAGKDVFISLDCAASEFFEKGVYTLEGKKLSPAEIVAFYEGIVKD
                     YPILSIEDGCSEGDKAGWKLITERLGGAIHLIGDDNFVTNPKILKQGIADGIANGLLV
                     KVNQIGTITETLEAVRMAQTAGYITVLSHRSGETEDTTIADLAVATNAGFIKTGSLCR
                     SERIAKYNRLLRIEEDLGETGVYPGLAAKKA"
BASE COUNT          383 a          722 c          675 g          299 t
ORIGIN      
        1 gcgacgacgc acggcctcga cgcggtcgcg aaggcccacg gcgcgcccgt cttcgagact
       61 gccgtcggct tcaagtacat cggcgagctg ctgctccagg acaggatcgt catgggcggc
      121 gaggagtcgg ccggcttcac gatggaaggg cacgtccccg agaaggacgg cccgctcgcg
      181 tgcctcctcc tcgccgagct cgtcgcgacg accggcaaga cgctcgggac gctcctgcag
      241 gagctcttcg cgagagtcgg gccgtactac ccggaccgcg ccgacgcgcg cctcgacccg
      301 aagctcgcgg aaacgatgaa gaagcggctc gcggaaaacc cggaaaactt cgccgggctg
      361 aaggttcagt ccatcgaccg gacggacggc cagaagctgc tcctcggcga cggccgctgg
      421 atcctcttcc gcgcctcggg gacggaaccc gtcgtgcgga tctacgcgga gtcttcggac
      481 aaacgagaaa caaacaggct tttggaagct gccaaagaat atgttcttag aagtaaatag
      541 ggcggcgggc gagcccagcg cacctcgagc ggcggcagcc cgcaccagcc tccggatatt
      601 caccttctaa gaagatcttc tcttcttctc tcttcctttt aggagcccac atgtacgcga
      661 tcgaaagcat ccacgcccgc gagatcctgg attcccgcgg caaccccacc gtcgaagtcg
      721 aatgcttgct cgagggaggc gcgtccgccc gcgcgggagt gccgtccggc gcgtccaccg
      781 gctcgcgcga ggccctcgag ctgcgcgacg gcgacaagaa gcgttacggc ggcaagggcg
      841 tcctcagggc cgtcaagaac gtgaacgaga cgatcgcgcc cgaggtcctc gggatggacg
      901 cgcgccagca ggccgagatc gaccgcctca tgatcgagct cgacggcacc gagatgaaga
      961 ccgcgctcgg cgccaacgcg atcctcgggg tctcgatggc cgtctgccgc gcggcggcgg
     1021 aggcctgcgc gctcccgctc ttccgttatg tcggcggcac gggcgccgtg acgctgcccg
     1081 tccccctcat gaacgtcgtg aacggcggcg cgcacgcgga caacccgctc gacatccagg
     1141 aattcatgct cgtccccgcg ggcttcgaca atttccgaga ggcgctgcgc gccggctcgg
     1201 aggtgttcca cgcgctcaag ggcctcctga aagcgaaggg cctcgcgacg ggcgtcggcg
     1261 acgagggcgg cttcgccccg aacctcgaat cgacgcgcgc gacgctcgac ttcctcgtcg
     1321 acgcgatcgg caaggcgggc tacaaggccg gcaaggacgt cttcatctcc ctcgactgcg
     1381 ccgcctcgga gttcttcgaa aagggcgtct acacgctcga gggcaagaag ctctcccccg
     1441 ccgagatcgt ggccttctac gaggggatcg tgaaggacta cccgatcctc tcgatcgagg
     1501 acggctgctc cgagggcgac aaggccggct ggaagctcat caccgaacgg ctcggcggcg
     1561 cgatccacct catcggcgac gacaacttcg tgacgaaccc gaagatcctc aagcagggca
     1621 tcgcggacgg catcgcgaac ggcctactcg tgaaggtgaa ccagatcggg acgatcacgg
     1681 agaccctcga ggccgtccgc atggctcaga ccgcgggcta catcacggtt ctctcgcatc
     1741 gctcgggcga gaccgaggac acgacgatcg ccgacctcgc cgtcgcgacg aacgccggct
     1801 tcatcaagac gggctcgctc tgccgctcgg agcggatcgc gaagtacaac cgcctcctgc
     1861 gcatcgagga ggacctcggc gagacgggcg tctaccccgg cctcgccgcg aagaaggctt
     1921 aaggccccga aacacgctcc gtcccggcgg aattgagcga aaatgatccg tgcgcttcga
     1981 acgtatcgtc ggcggtgaag gtccgccccg cctcgtcggc atcaagcgcg tcccccaggg
     2041 cccgcagcgt ccggcccggc cggagcgcga gcgcgagcc
//
LOCUS       DAOMFY010000448         2067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_27703_length_2067_cov_7.503976, whole genome shotgun sequence.
ACCESSION   DAOMFY010000448 DAOMFY010000000
VERSION     DAOMFY010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27703_length_2067_cov_7.503976"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLB01_19810"
     CDS             complement(<1..626)
                     /locus_tag="PLB01_19810"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HQQ79599.1"
                     /translation="MDANLGPWSPVAPVTFAVGATPATLRLAWDETALRVLFEIGDAT
                     HVTLGREDEDPDLGLKSDSVELFLDVRGEAAPRMDTNDYQFTVSRDGRRTTFKGSSAL
                     SGTVRPEMEADAAPQPKDWGMNVPIEVAVADGPEAGPGKSPGYRVEIAVPWAAVGGKP
                     ASGAAFGFSAAVNDLAMRPGQAAPMRTSVDFRGRSAFESPAVWAAAALR"
     gene            915..1421
                     /locus_tag="PLB01_19815"
     CDS             915..1421
                     /locus_tag="PLB01_19815"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQQ79600.1"
                     /translation="MTRFPGLLAAAALAAAFAFARTPGGPATEKGEKPDVTSRGGLRI
                     GSALVPWGGGAPVELKAADGTPAGPLVCVFNATYEMTNLGGGATGAPFTNRLRVDGAV
                     VATSSGLALNARETKSVATSPSLPAGIHTIELSIDDDSSVAESREDNNRFKVVYALRA
                     PCGTALKK"
     gene            complement(1430..>2067)
                     /locus_tag="PLB01_19820"
     CDS             complement(1430..>2067)
                     /locus_tag="PLB01_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79601.1"
                     /translation="ASAGWTAAAMALGWLLAAPFLLDLLWPVFLLLGIEHVTIERGAN
                     PFLNFSFDSYPWSHSLAMAVGWALLYGALAHFFTRSAAVGWVSSLGVVSHWICDAIVH
                     RPDLPLWPGSSIFVGMGLWNSVPGTLAVELPMFAAGVWLYAKATRPKDAVGRWSLAAL
                     VAVLLFFYAGSAFGPPPPNVAAVAIGTLALLVFVPWAVWIDRHREASDDPS"
BASE COUNT          334 a          753 c          730 g          250 t
ORIGIN      
        1 cgcagcgccg ccgccgccca cacggcgggc gactcgaagg ccgaccggcc ccggaagtcg
       61 accgacgtgc gcatcggagc ggcctggccg gggcgcatgg cgaggtcgtt caccgcggcc
      121 gagaagccga acgccgctcc ggacgccggc ttgcccccca ccgccgccca cggcacggcg
      181 atctcgaccc ggtagcccgg actcttcccg ggcccggcct cgggaccgtc cgcgacggcg
      241 acctcgatcg gcacgttcat tccccagtcc ttcggctgcg gggccgcgtc ggcctccatc
      301 tccgggcgca cggttcccga cagggccgac gatcccttga acgtcgtccg ccgtccgtcg
      361 cgtgaaaccg tgaactggta gtcgttcgtg tccatccgcg gcgccgcctc gccgcgcacg
      421 tcgagaaaga gctcgacgga gtcggacttg aggccgagat cggggtcctc gtcctccctc
      481 ccgagcgtga cgtgcgtggc gtcgccgatt tcgaacagca cgcgcagtgc ggtctcgtcc
      541 caggcgagcc gcagcgtcgc gggcgtcgcc ccgacggcga acgtcacggg cgcgaccggc
      601 gaccaggggc cgaggttggc gtccacgggc gacggcctct cgagaaacgg gagccgcagc
      661 ctctccccgg gacgggcaga cccgtctccc gcggcagcag cgactgccgc gagccctgcg
      721 gcgaggatga cggcgaggcg tcgattcagg tgcgcggagt ctatccgacg tctcagtctc
      781 ggggagccgc gctcgccggc cgggcgtggc cccggagcgc cacgggccca tctggcgctt
      841 ctgggcatgc gatcaacgcg ccgggaccac gcgcggcggt tgaaaggggc tcccggccgg
      901 agcatctttc actcatgaca cgctttcccg gccttctcgc cgccgccgct cttgccgccg
      961 ccttcgcctt cgcccggact cccggcggac ccgcgacgga gaagggcgag aaaccggacg
     1021 tcacgagccg gggcgggctc cgaatcggct ccgccctcgt cccatggggc ggcggcgcgc
     1081 cggtcgagct caaagccgcg gacgggacgc cggccgggcc cctggtctgc gtgttcaacg
     1141 cgacctacga gatgacgaac ctcggcggcg gcgcgaccgg cgcgcccttc acgaaccgac
     1201 tgcgcgtgga cggagccgtc gtcgcgacga gctcgggtct cgccctgaac gcccgcgaga
     1261 cgaagagcgt cgcgacctcg ccgtccctgc ccgccgggat ccacacgatc gagctctcga
     1321 tcgacgacga ttcgtccgtc gccgaatcgc gcgaggacaa caacagattc aaggtcgtgt
     1381 acgccctcag ggcgccgtgc ggtaccgctc tgaagaaata gctccgcctt caggaggggt
     1441 cgtccgaggc ctctcgatga cggtcgatcc agaccgccca cgggacgaag acgagaagcg
     1501 caagggtccc gatcgcgact gcggccacgt tcggcggcgg cggcccgaac gccgagcccg
     1561 cgtagaagaa caggagcacg gccacgagcg cggcgagcga ccagcggccc acggcgtcct
     1621 tcggacgcgt cgccttcgcg tagagccaga cgcccgctgc gaacatcggg agctcgacgg
     1681 ccagcgtgcc gggcaccgag ttccagagcc ccatgccgac gaagatcgag ctgccgggcc
     1741 agagggggag gtccggacgg tgaacgatcg catcgcagat ccagtggctc acgacgccga
     1801 gagacgagac ccagccgacg gccgcgctcc gcgtgaagaa atgcgcgagc gcgccgtaga
     1861 gaagcgccca gccgaccgcc atcgcgaggc tgtgggacca cgggtaggag tcgaaggaga
     1921 agttcaggaa cgggttcgct ccccgctcga tcgtgacgtg ctcgatcccg aggaggagga
     1981 agacgggcca gaggaggtcg agaaggaacg gagccgcgag gagccacccg agcgccatcg
     2041 ccgccgcggt ccacccggcg ctcgcga
//
LOCUS       DAOMFY010000449         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_28226_length_2047_cov_5.543675, whole genome shotgun sequence.
ACCESSION   DAOMFY010000449 DAOMFY010000000
VERSION     DAOMFY010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28226_length_2047_cov_5.543675"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLB01_19825"
     CDS             complement(<1..513)
                     /locus_tag="PLB01_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79602.1"
                     /translation="MRFEQVVRWLSRLFRTGLRPTTLAVLLLFRPAHGADGVSDVTAR
                     QIQALLSEKAARTPAQRKMDSQLLQAVRESRGQPMAAGVDLAPANVGTDVGGRVAVDV
                     AVRSAATVDAVIGRIEALGGEIVHPSWEHRTIRARIDLAQAEAVAGLPEVTFVQPAMG
                     HMHAQAARPAP"
     gene            complement(593..>2047)
                     /locus_tag="PLB01_19830"
     CDS             complement(593..>2047)
                     /locus_tag="PLB01_19830"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQQ79603.1"
                     /translation="APADAPRPDERLADPWAGFYAVLRRPVPFEELLQDLVTYVASAT
                     PLHSLAFLSLQPANEGEAIAAVWKGTLPKLSPKALSDARALGGPGDLVEGGARLKVFP
                     VLRPGADPVGVLVLPAETLSDPALFDFAAHASAAVGFVHAERSEAAFGEHAGAGHAAP
                     SPAPGGLDDPLSALVGASIALQNLKASLSRVASARAPLLLVGDVGSGKTLVAETLHAL
                     SPRRSRPLVRIAATAASPQALEEDLLGLEAAAGDRRRRPGRLFEADGGTLLIEEVGDL
                     PAPTQALLFRLLEAREVTAPGGRTVPMDVRVIGTTSRPLTRAVEDGHFRDDLYFRLSA
                     LTLRLPPLKDRKEDIPALLDALAARHGGPTAARFDVEAMNALLNYQYPGNVRELENEV
                     RRLAAQVGSGAVALADLDQKFSGEHVELALKESDDLKEIVEKVERQVIERVMRKVRGN
                     QSLGARLLNISRGSLIAKMKEYDIKDFRYLKRGD"
BASE COUNT          278 a          711 c          793 g          265 t
ORIGIN      
        1 gggcgccggc cgggccgcct gggcgtgcat gtgccccatc gccggctgga cgaacgtcac
       61 ctccggcagc ccggcgacgg cctcggcctg agcgaggtcg atgcgggcgc ggatcgtccg
      121 gtgctcccac gacgggtgga cgatctcgcc gccgagcgcc tcgatccggc cgatcacggc
      181 gtcgaccgtc gcggccgaac gcacggcgac gtccacggcg acgcggccgc cgacgtccgt
      241 gccgacattg gcgggcgcga ggtcgacccc ggcggccatc ggctgcccgc gcgactcgcg
      301 gaccgcctgc aggagctgcg agtccatctt ccgctgggcg ggcgtgcgcg cggccttttc
      361 cgagagcagc gcctggatct gccgggcggt cacgtcggag acgccgtccg cgccgtgtgc
      421 aggccggaag agcaggagga cggcgagggt cgtgggacgc aggcccgttc tgaagaggcg
      481 gctaagccag cggacgacct gttcgaatct catgcttcct cccggcgggt tggcgaaggg
      541 agcctacccc gcgggagagg gagtctcaac ccgaatgtcg gcgcggcgtt tcctagtcgc
      601 cccgcttcag gtaccggaag tccttgatgt cgtactcctt catcttcgcg atgaggctgc
      661 cgcgcgagat gttgaggagc cgcgccccga gagactggtt cccgcgcacc ttgcgcatga
      721 cgcgctcgat gacctggcgc tcgaccttct cgacgatctc cttgaggtcg tcgctctcct
      781 tcagcgcgag ctcgacgtgc tcgccggaga acttctggtc gaggtcggcg agcgcgaccg
      841 cccccgaccc gacctgcgcc gcgaggcgcc ggacctcgtt ctcgagctcg cggacgttgc
      901 ccgggtactg gtagttcagg agcgcgttca tggcctcgac gtcgaagcgc gccgccgtcg
      961 ggccgccgtg ccgcgccgcg agcgcgtcga ggagggccgg gatgtcttcc ttgcggtcct
     1021 tgagcggcgg cagacgcagc gtgagcgccg agaggcggaa gtagaggtcg tcgcggaagt
     1081 ggccgtcctc cacggcgcgc gtgagcggcc gggacgtcgt cccgatcacg cgcacgtcca
     1141 tcggcaccgt gcgtccgccg ggcgccgtga cctcccgcgc ctcgaggaga cggaagagga
     1201 gagcctgtgt cggcgccggc aggtcgccga cctcctcgat gagaagcgtg ccgccgtcgg
     1261 cctcgaagag gcgccccggg cgccgccggc ggtcgccggc ggccgcctcg aggccgagga
     1321 ggtcctcctc gagcgcctgc gggctcgcgg cggtggcggc gatccgcacg agcggccggc
     1381 tgcgacgcgg cgagagcgcg tgcagcgtct cggcgacgag cgtcttgccc gagcccacgt
     1441 cgcccacgag aaggagcggc gcgcgcgccg aggcgacgcg agaaagcgag gccttgaggt
     1501 tctgcagcgc gatggacgcg ccgacgaggg cggagagcgg atcgtcgagc cctcccgggg
     1561 cgggcgacgg agccgcgtgg cccgcaccgg cgtgctcgcc aaaggcggct tcgctgcgtt
     1621 cggcgtgcac gaatccgacc gcggcggagg cgtgggcggc aaagtcgaag agcgcggggt
     1681 ccgagagcgt ctccgcggga aggacgagga cgccgaccgg gtcggccccc gggcgcagga
     1741 ccgggaagac cttgaggcgc gcgccgccct cgaccaggtc gccgggcccc ccgagggcgc
     1801 gggcgtcgga cagcgccttc ggcgaaagct tcgggagcgt tcccttccac acggcggcga
     1861 tcgcctcgcc ctcgttcgcg ggctgcagcg agagaaacgc gaggctgtgg agcggcgtcg
     1921 ccgaggcgac gtacgtcacg aggtcctgga ggagctcctc gaacggcacg ggccggcgga
     1981 ggacggcata gaacccggcc cacgggtcgg cgaggcgctc gtcgggcctc ggggcgtcgg
     2041 caggagc
//
LOCUS       DAOMFY010000450         2041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_28375_length_2041_cov_8.626385, whole genome shotgun sequence.
ACCESSION   DAOMFY010000450 DAOMFY010000000
VERSION     DAOMFY010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28375_length_2041_cov_8.626385"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLB01_19835"
     CDS             <1..244
                     /locus_tag="PLB01_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79604.1"
                     /translation="AAVAFYRAAFALDGRPGHLASASFVENSLARCAEAQALADEAGL
                     AAQRPGVSDLDRHLATRAAAVAARCGVVPAGGEKPD"
     gene            complement(249..941)
                     /locus_tag="PLB01_19840"
     CDS             complement(249..941)
                     /locus_tag="PLB01_19840"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQQ79605.1"
                     /translation="MRRVLDGLTLFQRLAYPRHKDLFEHLAKNQTPQAVLITCSDSRI
                     VPDLLLQTEPGDLFIIRNAGNIVPPAGPTSGGTTASLEYAIVALGIRDVILCGHSNCG
                     AMNGVLHPEKLDGMPAVRRWVSYADLARRAALEAHPGATDEEMLEYVVDYNVIAQVRN
                     LLTFPFIRPLVEKGELEVYGWVYDIGSGRVKGLDATGRRFVPLGVDAMGSPDERHLLA
                     SVESDEEFWKKL"
     gene            993..>2041
                     /locus_tag="PLB01_19845"
     CDS             993..>2041
                     /locus_tag="PLB01_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HQQ79606.1"
                     /translation="MTHISSRSRRPAPVSRASLAILCAALLVPGEGALLAQAAPPAAP
                     AAAAEKPLTADHLDSLVAPIALYPDDLLAQTLAASTYPLEIIQLQQFLTKNKSLKDKA
                     LVDAVAKQPWDPAVQSMAAVPEVVKRLSDDIQWTTDLGNAFLDQQKDVMDACQRMRKK
                     AKDKGALASNEQMKVETKVVEQQTVIVVQSSNPEVIYVPTYSPTVVYPPPVYPYPPIY
                     YPPPPPPGAMFMTFTAGVMIGAAWGGSCCHCGWGSTSNVNINVNNNFNKNTNINRSGS
                     GGNNWSHNSAHRGGAPYSSSASANKYGGSTRGGSQGARGASPSASTASRGAGGGASPG
                     ASSASRGGGASPSASS"
BASE COUNT          335 a          729 c          696 g          281 t
ORIGIN      
        1 cgccgccgtc gcgttctacc gcgcggcgtt cgcgctggac gggaggcccg ggcacctcgc
       61 gagcgcctcg ttcgtcgaaa acagcctggc acgctgcgcc gaggcgcagg ctctcgcgga
      121 cgaggccggg ctcgccgcgc agcgcccggg tgtctcggac ctggaccgcc atctcgccac
      181 ccgcgccgcc gcggtcgcgg cccgatgcgg agtcgtgccg gccgggggcg agaagccgga
      241 ctgagctcct acagcttctt ccagaactcc tcgtcgctct cgacggacgc gaggaggtgc
      301 cgctcgtccg gagagcccat cgcgtcgacg ccgagcggca cgaagcgccg ccccgttgcg
      361 tcaaggcctt tcacgcggcc gctcccgatg tcgtagaccc acccgtagac ctcgagctcg
      421 cccttctcga cgagcgggcg tatgaacggg aacgtgagga ggttgcggac ctgcgcgatc
      481 acgttgtagt cgacgacgta ctcgagcatc tcctcgtccg tcgcgcccgg atgggcctcg
      541 agggcggcgc gccgcgcgag gtccgcgtac gagacccacc gccgcaccgc gggcatgccg
      601 tcgagcttct cggggtggag gacgccgttc atcgcgccgc agttcgagtg gccgcagagg
      661 atgacgtcgc ggatcccgag cgcgacgatc gcgtactcga gcgacgccgt cgtcccgccg
      721 gacgtcggcc cggcgggcgg gacgatgttt cccgcgttgc ggatgatgaa gaggtcgccg
      781 ggctcggtct ggagcaggag gtccggcacg atgcgcgaat ccgagcacgt gatgagcacg
      841 gcctgcggcg tctggttctt cgcgaggtgc tcgaagagat ccttgtggcg cgggtaggcg
      901 aggcgctgga agagcgtcag gccgtcgaga actcttctca cgccacgaag tctagcgccc
      961 cggattagga tcgcggggaa gggaaagagg ccatgaccca catttcatcc cgatcccggc
     1021 gcccggctcc cgtttcccgc gcttctctcg ccatcctctg cgcggcgctc ctggtcccgg
     1081 gcgagggcgc gctcctcgcg caggcggcgc cgccggcggc gccagccgcc gcggccgaga
     1141 agccgctcac ggccgaccat ctcgactctc tcgtcgcgcc gatcgccctc tacccggacg
     1201 atctcctcgc gcagacgctc gcggcgtcca cgtacccgct cgagatcatc cagctccagc
     1261 agttcctcac gaagaacaag agcctcaagg acaaggcgct cgtcgacgcc gtcgccaagc
     1321 agccgtggga tccggcggtc cagtcgatgg ccgcggtccc ggaggtcgtc aagcgtctct
     1381 cggacgacat ccagtggacc acggacctcg gcaacgcctt cctcgaccag cagaaggacg
     1441 tcatggacgc ctgccagcgg atgcgcaaga aggcgaagga caagggcgcc ctcgcctcga
     1501 acgagcagat gaaggtggag acgaaggtcg tcgagcagca gacggtcatc gtcgtccagt
     1561 cctcgaaccc cgaggtcatc tacgtcccga cgtacagccc gacggtcgtc tacccgccgc
     1621 cggtctaccc ctatccgccg atctactacc cgcctccccc gcctcccggg gccatgttca
     1681 tgacgtttac cgcgggcgtc atgatcggcg cggcctgggg cgggtcgtgc tgccactgcg
     1741 gctggggctc cacgagcaac gtcaacatca acgtcaacaa caacttcaac aagaacacga
     1801 acatcaaccg cagcgggagc ggcggaaaca actggtcgca caactccgcg caccgcggcg
     1861 gcgcgccgta ttcgagcagc gcttcggcga acaagtacgg cgggtcgacc aggggaggct
     1921 cgcagggagc gcgcggcgcg agcccgagcg cgagcaccgc gagccgggga gcaggaggcg
     1981 gcgcgagccc tggcgcaagc agcgcgagcc gcggaggggg cgcgagcccc agcgccagct
     2041 c
//
LOCUS       DAOMFY010000451         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_28723_length_2028_cov_7.528130, whole genome shotgun sequence.
ACCESSION   DAOMFY010000451 DAOMFY010000000
VERSION     DAOMFY010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28723_length_2028_cov_7.528130"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLB01_19850"
     CDS             complement(<1..848)
                     /locus_tag="PLB01_19850"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HQQ79607.1"
                     /translation="MFHNEPRPKRRRKLVLVESEPSAAPAPSGPGVPLLNRELSWLAF
                     NQRVLDEAFDERWPLLERLKFLCITETNLDEFFMIRVSGLLDQLSSEVVEHADDRLQP
                     AEALAQIRTTVQEMEARQTACLVADLLPKLAANGIAVVTWDALTEEQRTGATAYFRRN
                     VLPVLTPLAVDPGHPFPFLSNLSMNFAVEVKNPETGDVKFARVKVPQVLPRLLPLREI
                     LEGKKKVRPEKAEFLFLESLLQANLADLFPGLEIVSSHLFRVTRDADIEIQEDEASDL
                     LATIEQ"
     gene            complement(919..>2028)
                     /locus_tag="PLB01_19855"
     CDS             complement(919..>2028)
                     /locus_tag="PLB01_19855"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HQQ79608.1"
                     /translation="HAGLLALADEAARAGRLTKFVPASGAASRMFAFLLAGGEETAER
                     KRFFESREAFAFGSSGDLAALAARPKALVPFHTYADGPRTPFEEHLHEAAGTVRDAAG
                     LCRVHFTVSPEHRKAFEDRLAEVRPRVERATGARFSVSFSEQSPATDTIAGGADGGPF
                     RTASGGLLFRPGGHGALLKNLGDVARAGGDVVLVKNVDNVVPDARRGPTLLWKRLLTG
                     LLVKLERESRRDRPIRVCGVVRNEGEPGGGPFWVSGPRGETLQIVESAQVDLADPAQA
                     AIWKTATHFNPVDLACSLRDALGQPFDLGPFVDESAVFIAEKTHEGMPLKALERPGLW
                     NGAMAFWETVFVEVPKETFAPVKTVLDLLRPEHRA"
BASE COUNT          307 a          666 c          751 g          304 t
ORIGIN      
        1 tcctgctcga tcgtcgcgag gaggtccgac gcctcgtcct cctggatctc gatgtcggcg
       61 tcgcgcgtga cgcggaagag gtgcgaggag acgatctcga ggcccgggaa gaggtccgcg
      121 aggttcgcct gcagcaggga ctcgaggaag aggaactcgg ccttctccgg ccggaccttc
      181 ttcttgccct cgaggatctc gcgcagcggc aggagccgcg ggaggacctg cgggaccttc
      241 acgcgcgcga acttcacgtc gccggtctcg gggttcttca cttcgacggc gaagttcatc
      301 gagaggttcg agaggaacgg gaacgggtgg cccgggtcca cggcgagggg cgtcaggacc
      361 ggcaggacgt tccgccggaa gtaggccgtg gcgcccgtgc gctgctcctc cgtgagggcg
      421 tcccaggtca cgacggcgat cccgttcgcg gccagcttcg ggaggaggtc ggccacgaga
      481 cacgcggtct ggcgcgcctc catctcctgg accgtcgtcc ggatctgcgc gagcgcctcg
      541 gccggctgga ggcggtcgtc cgcgtgctcg acgacctcgg acgagagctg gtcgaggagc
      601 cccgacacgc ggatcatgaa gaactcgtcg aggttcgtct ccgtgatgca gaggaacttc
      661 agccgctcca ggagcggcca gcgttcgtcg aacgcctcgt cgaggacccg ctggttgaac
      721 gcaagccacg agagctcgcg gttcaggagg ggaacgccgg gtcccgacgg cgcgggcgca
      781 gccgacggct cggattcgac gaggacgagc ttgcggcgcc gcttggggcg gggttcgttg
      841 tggaacatgg cgggcccccc gattccaggg ggagggaagg atacgcccgc cgggtgaagg
      901 gggcgtttta cgcggcgctc aggcgcgatg ctcgggccgg aggaggtcga gaacggtctt
      961 gaccggggcg aacgtctcct tcgggacctc gacgaaaacg gtctcccaga acgccatggc
     1021 gccgttccat aaccccggcc tttccagtgc tttgagcggc attccctcgt gcgtcttctc
     1081 ggcgatgaag acggcggact catccacgaa agggccgagg tcgaagggtt gtcccagggc
     1141 gtccctgagg gagcacgcga ggtcgaccgg gttgaagtgg gtggcggtct tccagattgc
     1201 agcctgggca gggtccgcga ggtcgacctg ggccgactcg acgatctgga gggtctcgcc
     1261 cctcggcccc gaaacccaga acggcccgcc gccgggctcg ccctcgttgc gcacgacgcc
     1321 gcagacccgg atcgggcggt cgcggcggct ctcgcgctcg agcttgacga ggagacccgt
     1381 caggagccgt ttccagagga gcgtcggtcc gcggcgcgcg tccggcacga cgttgtccac
     1441 gttcttgacg aggacgacgt cgccgcccgc gcgcgccacg tcgccgaggt tcttcaggag
     1501 cgcgccgtgg ccgccgggac ggaagaggag gccgccggag gccgtgcgga acgggccacc
     1561 gtccgcgccg ccggcgatcg tgtcggtcgc gggggactgc tccgagaacg acaccgagaa
     1621 gcgcgctccc gtcgcgcgct ccacgcgcgg ccggacctcg gcgagcctgt cctcgaaagc
     1681 ctttcgatgc tcgggcgaca cggtgaaatg gacgcggcag agcccggcgg cgtcgcgcac
     1741 ggtgccggcg gcctcatgaa gatgttcttc gaaaggggtt cttgggccgt ccgcgtacgt
     1801 gtggaacggc acgagggctt tgggtctcgc cgcgagagcc gcgagatcgc cggagctgcc
     1861 gaatgcaaac gcctcccggc tttcgaagaa tctcttcctc tcggccgtct cctctccacc
     1921 cgcgaggagg aacgcgaaca tccggctcgc cgcgcccgac gccggcacga acttcgtgag
     1981 ccggcccgcg cgggcggcct cgtcggcgag cgcgaggagg cccgcgtg
//
LOCUS       DAOMFY010000452         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_28964_length_2019_cov_6.450611, whole genome shotgun sequence.
ACCESSION   DAOMFY010000452 DAOMFY010000000
VERSION     DAOMFY010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28964_length_2019_cov_6.450611"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="PLB01_19860"
     CDS             complement(<1..159)
                     /locus_tag="PLB01_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQQ79609.1"
                     /translation="MEYRQLGGSGFKVPVLSFGTGTFGGGGELFKAWGASDVAEATRL
                     VDICLEAGL"
     gene            complement(289..972)
                     /locus_tag="PLB01_19865"
     CDS             complement(289..972)
                     /locus_tag="PLB01_19865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HQQ79610.1"
                     /translation="MALIMGMTIMAILIAAVLPLASTEAQRDKEDELIFRGFQYAEGI
                     RTFRRRYGRYPNTLKEMFEVRPRTLRKLWKDPMTNSLDWGLVSVTTGAPLPGTTPPRP
                     PGGASPTPVPTAAPTPGFGANNHDQLLITGGAFLDGTVTVQLINGYTPTEGTVIEFMR
                     YGSVTGNFAQFVGLNIGGGLSFLPVRNAGNYQFVVVPEPASVLGVGAAGLALGVWVRR
                     RRKVEPAVG"
     gene            complement(1010..1411)
                     /locus_tag="PLB01_19870"
     CDS             complement(1010..1411)
                     /locus_tag="PLB01_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQQ79611.1"
                     /translation="MRHPKGFTLVELLVVMAILGILVAIAVPQLQQAPIRAKEATLRE
                     DLFTFRTCLDQFYADKGHYPETLQTLVTEKYLRKIPVDPFTKSADTWQVVMEEPDSSE
                     TGSADQPPGIIDVKSGSKEISRIDKTAYNTW"
     gene            complement(1414..1881)
                     /locus_tag="PLB01_19875"
     CDS             complement(1414..1881)
                     /locus_tag="PLB01_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQQ79612.1"
                     /translation="MTLASRTRRGYSLVELVIVCACILILTAMIVPVTRFGVVRQKEM
                     ELRAALRNIRTAIDDFKRSSDQGLIPVELDTEGYPKTLEKLVEGVDLVGQIKKKKRFL
                     RKIPIDPMTGKAEWGLRSYQDEPDARSWGRQNVYDVYSLSPLTGLDKTKYKDW"
BASE COUNT          318 a          688 c          659 g          354 t
ORIGIN      
        1 caaaccggct tcgaggcaga tatcgacgag ccgggtcgcc tcggccacgt cgctcgcacc
       61 ccaggctttg aagagttcgc cgccgccgcc gaaggtgccg gtcccaaaac tgagtacggg
      121 cactttgaac cccgaaccgc cgagttgccg gtattccatg gtccgctcat ttccgaagtc
      181 gtggagtaaa tcaagcgcag cgatcggcca tacgtgtacg gccactgcgt gttatacacg
      241 ccgaatggtg cttcgaattg atggcgagac tcggcggggg ccacggtttc aaccgacggc
      301 gggttcgact ttccgtcgcc gacggaccca cacgccgagg gccaatcccg ctgccccgac
      361 ccccagcacg ctcgcgggct cgggcacgac cacgaactgg tagttgcccg cgttccgcac
      421 cgggaggaac gacaggccgc cgccgatgtt caggccgacg aattgtgcga agttgcccgt
      481 caccgagccg tagcgcatga actcgataac ggtgccctcc gttggcgtgt agccgttgat
      541 gagttgcacc gtcaccgtgc catcgaggaa cgcgccgccg gtaatcagaa gctgatcgtg
      601 gttgttcgcg ccgaagccgg gcgtcggggc cgccgtcggg accggcgtcg gcgacgcccc
      661 gccgggcggc cgcggcggag tcgttccggg cagcggggcg ccggtcgtca ccgacacgag
      721 gccccagtcg agggaattcg tcatggggtc cttccacagc tttcggagcg tgcgcggacg
      781 tacctcgaac atttccttga gcgtattcgg gtagcgcccg tagcggcgcc tgaaggtccg
      841 gatgccctcg gcgtactgga acccgcggaa aatgagctcg tcttccttgt cgcgctgggc
      901 ctcggtcgaa gcgagcggaa gcaccgcggc gatgaggatc gccatgatcg tcatgcccat
      961 gatcagggcc accagcgtgt agccgcgggt ccccctcacc gctcccccgt caccacgtgt
     1021 tgtaggccgt cttgtcgatc cgcgagatct ccttcgagcc gctcttgacg tcgatgatcc
     1081 ccggaggctg gtcggcggac ccggtctccg acgagtccgg ctcctccatc acgacctgcc
     1141 acgtgtcggc cgacttcgtg aacgggtcga ccgggatctt gcggaggtac ttctccgtga
     1201 cgagcgtctg cagcgtctcg gggtagtgcc ccttgtccgc gtagaactgg tccaggcagg
     1261 tccggaacgt gaagaggtcc tcgcgcagcg tcgcctcctt cgcgcggatg ggcgcctgct
     1321 gcagctgcgg aacggcgatc gccacgagga tcccgagaat cgccatcacg acgaggagct
     1381 cgacgagcgt gaagcccttc gggtgtctca tgctcaccag tccttgtact tcgtcttgtc
     1441 gagcccggtg agcggcgaga gcgagtacac gtcgtacacg ttctgccgcc cccacgagcg
     1501 cgcgtcgggc tcgtcctgat agctccggag gccccactcg gccttccccg tcatcgggtc
     1561 gatcgggatc ttcctcagga accgcttctt cttcttgatc tggcccacga ggtcgacgcc
     1621 ctcgacgagt ttctcgagcg tcttcgggta gccctcggtg tccagctcga ccggaatcag
     1681 gccctggtcg gacgagcgct tgaagtcgtc gatcgccgtc cggatgttcc ggagcgccgc
     1741 gcggagctcc atctccttct gacgcacgac tccgaaccgc gtgaccggca cgatcatcgc
     1801 cgtcaggatc aggatgcacg cgcacacgat cacgagctcg acgagcgagt agccgcggcg
     1861 cgtgcgggaa gcgagcgtca tggagcgtcc ttcaccgtga acggggccgc ctcgacgacc
     1921 ggagcgggcg cggccccctt cgccgtcgcg gccgcgccgg cggtcacgcg gacgagcgtc
     1981 gtcccgggcg cggtcccctt caccgcgagc ttcgcgagg
//
LOCUS       DAOMFY010000453         2013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_29116_length_2013_cov_4.593463, whole genome shotgun sequence.
ACCESSION   DAOMFY010000453 DAOMFY010000000
VERSION     DAOMFY010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29116_length_2013_cov_4.593463"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..550
                     /locus_tag="PLB01_19880"
                     /pseudo
     CDS             1..550
                     /locus_tag="PLB01_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
     gene            619..942
                     /gene="yajC"
                     /locus_tag="PLB01_19885"
     CDS             619..942
                     /gene="yajC"
                     /locus_tag="PLB01_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQQ79613.1"
                     /translation="MNPSLLQAAPAGIAANPIVQMVPYLLMFVIFYFVLLAPMRKQQK
                     KQKELLSALKKGDRVVTSGGIHGTIAQVEDQIVWLKIADTTKIKINRSAIAGLSADTE
                     TKEPA"
     gene            971..>2013
                     /locus_tag="PLB01_19890"
     CDS             971..>2013
                     /locus_tag="PLB01_19890"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQQ79614.1"
                     /translation="MPLGRPLTLLQSSDWHVGSALTGGGLGMPPEVRAVRREEVDGAA
                     ERAVEAAKARGADGLVVPGDLWDAENVPAAAVHRLLEAFASFAPRPVFVSPGNHDFAG
                     AGGWYDPSVLAALGMRAWPENVVVFRDPAWATVPFPGRDDATVTGRAFLSPAVVVDRP
                     LSPPPKRPDSVHALLMLHGSLESYPGADSPRGAKRTAPFSRREVVDASFSWAALGHHH
                     HVCFVEDDAGVLRGAYSGCPTGRGLDETGPRVFLEVTLVPGEPPRVETLPADSRVVHD
                     LSVDATDLEGAALAERVETAWAVAGVGPPDLVRLTLTGTQPFGARPAETARAFDGTGL
                     DAVLKWPNDVLAQP"
BASE COUNT          255 a          754 c          715 g          289 t
ORIGIN      
        1 ggccgcccgg cgcgaggcgc tcgcctcgcc gcgcggcgcg ctgttcggga tcgtgcaggg
       61 cggcatgtac ccggacctgc gcgcggagag cgccgccgcg atcgccgcgc tcccgttcga
      121 cggcgtcgcc tgcggcgggc tctcggtggg ggagccgaag gacgagatgc gcgcgatggt
      181 cgcggcgacc gctccgctcc tgccggaaga gcggccccgc tatctcatgg gcgtcgggcg
      241 gcccgacgac ctcatcgacg ccgtggcgcg gggattcgac ctcttcgact gcgtgctccc
      301 gacgcgtgcc gcgcggcatg gcctcctcta cacctccgag gggacgctcg cgatcaagca
      361 cgcccgctac cgcgaggatg cggcgccgcc cgaccgctcg tgcgggtgcg cgacgtgccg
      421 gaggcactcg cgcgcgtacc tccggcacct gttcgtcgcc ggcgagccta cggccgcgac
      481 gctcctcacg gtccacaacc tcacggccat ccttgacttg atgcggggaa tccgtgaggc
      541 tctcgcctcg aatcggttca cggcgtgggc ggcggaagcg cttccccgct ggtcgcgagc
      601 cgtcggaaag gactcctgat gaacccgtcc ctcctgcagg ccgcccccgc cggcatcgcg
      661 gccaacccga tcgtgcagat ggtcccgtac ctgctcatgt tcgtgatctt ctacttcgtg
      721 ctgctcgcgc cgatgcgcaa gcagcagaag aagcagaagg agctgctctc ggccctgaag
      781 aagggcgatc gcgtcgtcac gtcgggcgga atccacggga cgatcgcgca ggtcgaggac
      841 cagatcgtct ggctcaagat cgccgacacg acgaagatca agatcaaccg cagcgcgatt
      901 gcgggcctct cggccgacac ggagacgaag gaacccgcct gatctgcgcc cgtccgtgag
      961 aaaatgaggg gtgccactcg ggcgacctct caccctcctc cagtcctcgg actggcatgt
     1021 gggctccgcg ctcacgggcg gggggctcgg aatgccgccg gaggtgcgcg ccgtccggcg
     1081 tgaagaagtc gacggcgccg ccgagcgcgc cgtcgaggct gcgaaggccc gcggcgccga
     1141 cggcctcgtc gtccccggcg acctctggga cgccgagaac gtgcccgccg cggcggtcca
     1201 ccgcctgctc gaggcgttcg cctcgttcgc gccgcggccc gtcttcgtct cgcccggcaa
     1261 ccacgacttc gcgggagccg gcggctggta cgacccgtcg gtcctcgcgg cgctcggcat
     1321 gcgggcgtgg ccggagaacg tcgtggtctt ccgcgatccc gcgtgggcga cggtgccgtt
     1381 cccgggccgg gacgacgcga ccgtcacggg ccgtgcgttc ctgtcgcccg cggtcgtcgt
     1441 cgaccgcccg ctctcgccgc ctccgaagcg ccccgattcc gtgcacgcgc tgctcatgct
     1501 ccacggttcg ctcgagagct atccgggggc ggactcgccg cgcggggcca aacgcacggc
     1561 gcccttctcg cggcgcgagg tcgtcgacgc ctccttctcg tgggcggcgc tcggccacca
     1621 ccaccacgtc tgcttcgtcg aggacgacgc gggcgtgctc cgcggggcgt actcgggctg
     1681 cccgaccgga cggggcctcg acgagacggg tccccgcgtg ttcctcgagg tcacgctcgt
     1741 ccccggagaa ccgccgcgcg tcgagacgct ccccgcggac tcccgcgtcg tccacgacct
     1801 ttcggtcgac gcgacggatc tcgagggggc ggcgctcgcc gagagggtcg agaccgcgtg
     1861 ggccgtcgcc ggggtgggcc cgccggacct cgtgcggctc acgctcaccg ggacgcagcc
     1921 gttcggcgcg cggcccgcgg agacggcgcg cgcgttcgac ggcaccggcc tcgacgccgt
     1981 gctcaagtgg cccaatgacg tgctggcgca gcc
//
LOCUS       DAOMFY010000454         2002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_29377_length_2002_cov_4.486903, whole genome shotgun sequence.
ACCESSION   DAOMFY010000454 DAOMFY010000000
VERSION     DAOMFY010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29377_length_2002_cov_4.486903"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLB01_19895"
     CDS             complement(<1..246)
                     /locus_tag="PLB01_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79615.1"
                     /translation="MSRAANGEKGGTGGGNCAAAARVRAAIDLSLSEENSSSSSSLPS
                     LPSDLKAHLSACAACAAEAAALDPTLVFAPLAASVSPE"
     gene            complement(286..951)
                     /locus_tag="PLB01_19900"
     CDS             complement(286..951)
                     /locus_tag="PLB01_19900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ79616.1"
                     /translation="MRLVSASARSLSPASDAPGESVAAEGAASDAPTVERALALRAQA
                     GDLEAFAALVERRTPGVVAFLRRMLGDAEDARDVAQITFLRVWENIGRYDPAWAFSTW
                     LFRIAGNLAIDALRARKTRQRTETESFRLVKGARTSAESDALGALHRDEVRRVFENCA
                     GVLSETQRLVFVLREFEEKDGRDIAAILECRESTVRNHLFQARRLLRDEIRKRYPEYT
                     SGF"
     gene            1203..>2002
                     /locus_tag="PLB01_19905"
     CDS             1203..>2002
                     /locus_tag="PLB01_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQQ79617.1"
                     /translation="MSLTLATELTRRRPSIPLLLSATTPAGVGLLSRRLASASLPWRP
                     FPLDLPSAVRRFFDAERPRLLVLVETELWPAVLNEARRRGVPVLLANARLSERSAARY
                     RRASALFSGPLSALTRVLARTDVDAARFVEIGVPAARVVASGDMKFDHAVLMAPHQAG
                     DLAWKAGAIGTNAEGKPSGWAMVDPLMLTLKNDPDVYVIGDAVGMVSPQFLFYPKAGH
                     VANAHGRIVAGYIAERLAGRAPKPALPDNLCYMLVNGEPRESVAVQFD"
BASE COUNT          288 a          730 c          667 g          317 t
ORIGIN      
        1 ctcgggcgag acggacgccg cgagcggcgc gaagacgagc gtcgggtcga gcgccgcggc
       61 ctcggcggca cacgcggcgc acgccgaaag atgcgctttc aggtcggagg ggagagacgg
      121 aagagaagaa gaagaagaag aattttcttc tgaaagggaa agatcgatgg cggcgcgaac
      181 acgggcagcg gcggcgcaat ttccgccgcc cgtcccgccc ttctcaccgt tcgccgccct
      241 gctcatctcc ctctccttct tacctttcta agaaatccga aagacctaga aacccgacgt
      301 gtattcggga taccgcttcc ggatctcgtc gcgcaggagg cggcgggcct ggaagaggtg
      361 gttgcgcacc gtggactcgc ggcactcgag gatcgcggcg atgtccctgc cgtccttctc
      421 ctcgaactcg cggagaacga agacgagccg ctgcgtctcc gacaggacgc ccgcgcagtt
      481 ctcgaacacc cggcgcacct cgtcgcggtg aagggcgccg agggcgtcgg actcggcgct
      541 cgtccgcgcg cccttcacga gacggaagct ctcggtctcg gtccgctggc gggtcttgcg
      601 cgcccgcagc gcgtcgatcg cgaggttgcc ggcgatccgg aacagccacg tcgagaacgc
      661 ccacgcaggg tcgtagcgcc cgatgttctc ccagaccctc aggaacgtga tctgcgcgac
      721 gtcgcgcgcg tcctcggcgt cgccgagcat ccggcggaga aacgccacga cgccgggcgt
      781 ccgccgctcg acgagagccg cgaaggcctc gaggtcgccc gcctgcgccc ggagcgcgag
      841 cgcccgctcg acggtcgggg cgtcggaggc ggcgccctcc gcggccacac tttccccggg
      901 cgcgtcggac gcgggcgaaa gagagcgcgc ggaggccgag acgagacgca tcgcttcagc
      961 ctctgttacg tttgccggat cgtcttcgtc tatcaggtaa agaggcctcc gggccgctcg
     1021 gcagtataat ccgccacggc ttgccgacgc atcttaaatt cttgtcccga ctcgatttga
     1081 gcgccactct ccccgccgag gccccgctct ttcctctcgt attttcgcgc cttttcccca
     1141 gccggaccgg actcgttccg ggcggggtct ggatccaggg ggtctcgctc ggcgaggtcg
     1201 aggtgtccct cacgctcgcc accgagctga cccgacgccg gccctccatc cctctccttc
     1261 tgagcgccac gacgcccgcc ggcgtcggcc tcctgtcgcg gaggctcgcc tccgcgtcgc
     1321 tgccgtggcg gccgtttcca ctcgatctcc cgtccgccgt gcggcgcttc ttcgacgcgg
     1381 agcgcccgcg gctgctcgtc ctcgtggaaa cggagctctg gcccgccgtg ctgaacgagg
     1441 cgcgccgccg cggggtcccg gtgctcctcg cgaatgcgcg cctctccgag cgctcggccg
     1501 cacgctaccg ccgcgcgtcg gctctcttct ccggccccct ctcggcgctc acgcgcgtcc
     1561 tcgcgcgcac ggacgtggac gcggcgcgtt tcgtggagat cggcgtcccc gccgcccgcg
     1621 tcgtggcgtc cggcgacatg aagttcgacc acgcggtgct gatggcgccg caccaggcgg
     1681 gcgacctggc ctggaaggcc ggcgccatcg gcaccaatgc cgaaggcaag cccagcggct
     1741 gggcgatggt ggacccgctg atgctcaccc tcaagaacga cccggacgtc tacgtcatcg
     1801 gtgatgcggt gggcatggtc tcgccgcagt tcctgttcta tcccaaggcc gggcacgtgg
     1861 ccaacgccca cgggcgcatc gtggccggct acatcgccga gcgcctggcc ggccgggcgc
     1921 ccaagccggc gctgcccgac aacctctgct acatgctggt caacggcgag ccgcgcgaat
     1981 cggtggcggt gcagttcgac ta
//
LOCUS       DAOMFY010000455         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_29600_length_1993_cov_6.029412, whole genome shotgun sequence.
ACCESSION   DAOMFY010000455 DAOMFY010000000
VERSION     DAOMFY010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29600_length_1993_cov_6.029412"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLB01_19910"
     CDS             complement(<1..187)
                     /locus_tag="PLB01_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79618.1"
                     /translation="MKRKRALAVGAACLILLRPSSSAQQPTPPSKKSESPSPSSSLSS
                     SSADLFSPGPEGSRNLVV"
     gene            complement(238..1464)
                     /locus_tag="PLB01_19915"
     CDS             complement(238..1464)
                     /locus_tag="PLB01_19915"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQQ79619.1"
                     /translation="MSDIFPDPPDPPRPPNPPKPPLSLPQVPLDWMKRVPVRSVLLGA
                     ALVFTVWSGARWAERSFQSVAPGEAGLVVSKFTGGTRIVAPGSHFLPATFYVLTRMRV
                     SDQLLSGPSATFTVPTKDGFAVGLVLQARWALDRNQLLTRWAGLPANPGTDVVGPVLA
                     SAFRNLAPEYDAPSLLASKREELAGRAAKQAGDRLAEAGILLKDVLVSDLKLPAEFER
                     GRLALLEQAQLVERTEANLRFKKQEIEQQRLEAEARKARSDKEAEAAANRRLIEAKAE
                     ADAMTYILPLKEKAIKQQELEADAQKARRLREAESAAATMKIQSEAEAARRRTLSEAE
                     AFAIRQTSLAQFENLKREVELIEKNPTWVNKTLAEKLGDKVQVIVMPNLTADILGKEA
                     GKRVANGQPALASRAD"
     gene            1702..>1993
                     /locus_tag="PLB01_19920"
     CDS             1702..>1993
                     /locus_tag="PLB01_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79620.1"
                     /translation="MVRRYVAPILRLLAFLGLLASPLLLAAIARADGAAAPPAAVLQG
                     TRLVALRSVVLADRPDDRVARGGRVAASAPATLLERVGGKVLVTSPGWGGSVA"
BASE COUNT          274 a          663 c          725 g          331 t
ORIGIN      
        1 cgacgacgag gttcctgctg ccctcgggac ccggggagaa gaggtctgct gaagaagaag
       61 aaagagaaga agaaggggat ggagattcgg atttcttgga aggtggcgtg ggctgctgcg
      121 cgctcgacga cggccgcagc aagatgaggc aggcagctcc gacggcgagg gccctcttcc
      181 ttttcataga aatcttctct ttctcttttc tctctctctt cttcttcctt ttcctgctca
      241 atccgcccgc gaggcgagcg cgggctggcc gttcgcgacg cgcttgccgg cttccttgcc
      301 gaggatgtcg gcggtgaggt tcggcatgac gatgacctgc accttgtcgc cgagcttctc
      361 ggcgagcgtc ttgttgaccc acgtcgggtt cttctcgatg agctcgacct cgcgcttgag
      421 gttctcgaac tgcgcgagcg acgtctggcg gatggcgaac gcctccgcct cggacagcgt
      481 tcggcggcgc gccgcctcgg cttccgactg gatcttcatc gtcgccgcgg ccgattccgc
      541 ctcgcgcagg cggcgcgcct tctgggcgtc cgcctcgagc tcctgctgct tgatcgcctt
      601 ctccttgagc gggaggatgt acgtcatcgc gtcggcctcg gccttggcct cgatgaggcg
      661 cctgttcgcg gccgcctcgg cctctttgtc cgagcgcgcc ttgcgcgcct cggcctcgag
      721 gcgctgctgc tcgatctcct gcttcttgaa ccggaggttc gcctcggtac gttccacgag
      781 ctgggcctgc tcgaggaggg cgaggcgccc gcgctcgaac tcggccggga gcttcaggtc
      841 gctcacgagg acgtccttca ggaggatccc ggcctcggcg aggcggtcgc cggcctgctt
      901 cgcggcgcgg cccgcgagct cctcccgctt gctcgcaagg agcgacggcg cgtcgtactc
      961 gggcgcgagg ttgcggaagg ccgacgcgag gaccgggccg acgacgtcgg tgcccgggtt
     1021 cgcgggaagc ccggcccagc gcgtgaggag ctggtttcgg tcgagcgccc agcgcgcctg
     1081 gaggacgaga ccgaccgcga acccgtcctt cgtcgggacc gtgaacgtcg ccgaaggccc
     1141 ggagaggagc tggtccgaga cgcgcatgcg cgtcaggacg tagaaggtgg ccggcaggaa
     1201 atggctgccc ggcgccacga tgcgcgttcc gccggtgaac ttgctcacga cgaggccggc
     1261 ttcgccggga gcgacggact ggaagctgcg ttcggcccag cgggcgccgg accagacggt
     1321 gaagacgagg gccgcgccga ggaggacgga gcgcacggga acgcgcttca tccagtcgag
     1381 cggaacctgg ggaagagaga ggggaggctt cgggggattc ggagggcggg gaggatcggg
     1441 cgggtcgggg aagatgtcgc tcatgtcgtc tccttcgcgg cgtgaacgcc gctgaggaag
     1501 agcaatccac gaagggtgcc aaggggcgaa cgcgcccggc gcgctccgcc cgcgcaaact
     1561 catcttccta ttcgcagaaa tcttctccga atcgcataat cgagtcgcgc cgaggcccgc
     1621 gtgggcaggg cgggacccgg cggcacgcga cgtgaaaagg cggagggcag gcgcgagaac
     1681 accccgcgcc gggaggttca catggttcgc cgctacgtcg ccccgatcct caggctcctc
     1741 gcgttcctcg gcctgctcgc gtctccgctc ctccttgccg cgatcgcccg ggcggacggc
     1801 gccgccgcgc cgcccgcagc cgtgctgcag ggaacgcgcc tcgtggccct gcgctcggtc
     1861 gtcctcgcgg accggccgga cgaccgcgtc gcccgcggcg ggcgcgtcgc ggccagcgct
     1921 cctgcgacgc tcctcgagcg cgtgggcggc aaggtgctcg tgacgtcgcc cggctggggg
     1981 gggagcgtcg ccg
//
LOCUS       DAOMFY010000456         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_29706_length_1989_cov_7.927094, whole genome shotgun sequence.
ACCESSION   DAOMFY010000456 DAOMFY010000000
VERSION     DAOMFY010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29706_length_1989_cov_7.927094"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLB01_19925"
     CDS             <1..219
                     /locus_tag="PLB01_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HQQ79621.1"
                     /translation="FNGVTGIFETDKGDDFIVGHGLFRAAGQPVSAVRYVDLYLNNLG
                     VIKEGVSKDEAASIKKKFEDAGAKVEVK"
     gene            350..>1989
                     /locus_tag="PLB01_19930"
     CDS             350..>1989
                     /locus_tag="PLB01_19930"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQQ79622.1"
                     /translation="MIGRTPASGSKERTSFGKIRSFLPLPNLIDVQRKSYEDFLQMDL
                     LPEERQDVGLQAVFKSVFPFSDFRETCALEFVSYRIGEWKSRSGRLEGLQHLRFSCEH
                     CGATVKVKDPRATRVECGACGHPNANKVTIDEVCGTPVELRLPYSVAECQERGSTYSV
                     PLKVTFRLKVFDKENAPTGKGAPKEWPIRDIKEEEVYFGEIPLMTDNGTFVINGTERV
                     IVSQLHRSPGVFFTREGAHTLIGKIIPYRGSWVEFEIDPKGLFGVRIDRKRKFPGTVF
                     LRALGLESDELILKKFYSPVKVSFERGKARLVLPPEVLTKEELRVKHLRGIRKDGRIF
                     ADVKLTAEMKASLAKGESVEVSVDPAKLGRALFAADVVDLGSGEVLFETGSEIPEDLA
                     AQLENKQATAVEAIFPDWEPIGETLIATLRKDGVKTKREALLEIYKRMRPGDPPTDES
                     AKNLFAGMFFDPRKYDFSRVGRFKFNIRTGLQTSLDQKTLTAEDFYVVIQHLLELKRD
                     IGKTDDIDNLGNRRVRCVGELLENQFRIGLVRMERAIK"
BASE COUNT          362 a          667 c          642 g          318 t
ORIGIN      
        1 ttcaacgggg tgacgggcat tttcgagacg gacaaggggg acgacttcat tgtcggccat
       61 gggttgttcc gggcggccgg ccagccggtg tcggccgtgc gctacgtgga cctctatctc
      121 aacaacctcg gcgtcatcaa ggagggcgtc tcgaaggacg aggccgcttc catcaagaag
      181 aagttcgagg acgccggagc gaaggtcgag gtcaagtaac ccggaggggg tgcgggggaa
      241 ccgaggttcg gttcccccgc cactggccgt ttctctcgat tttcgccgtc gccccatcgc
      301 gcgcttcaca ccccggcctg cgcgcggcca tgagaggaca tagcacccca tgatcggacg
      361 cacccccgcc tccggttcga aagaacggac gagcttcggc aagatccgct ctttcctgcc
      421 ccttcccaac ctcatcgacg tccagcgcaa gtcgtacgag gacttcctcc agatggacct
      481 cctgcccgaa gagcggcagg acgtcggcct gcaggccgtc ttcaagagcg tgttcccgtt
      541 cagcgacttc cgggagacgt gtgcgctgga gttcgtgtcg taccgcatcg gcgagtggaa
      601 gtcccgcagc ggccggctcg aaggcctcca gcatctgcgt ttctcgtgtg agcactgcgg
      661 cgccaccgtc aaggtcaagg acccgcgcgc gacgcgcgtc gagtgcggtg cctgcggcca
      721 cccgaacgcg aacaaggtca cgatcgacga ggtttgcggg accccggtcg agctccgcct
      781 cccgtactcg gtcgccgagt gccaggagcg cggctcgacg tactcggtgc cgctcaaggt
      841 gacgttccgc ctcaaggtgt tcgacaagga gaacgctccg acggggaagg gtgccccgaa
      901 ggagtggccg atccgcgaca tcaaggagga agaggtctac ttcggcgaga tcccgctcat
      961 gacggacaac ggaacgttcg tcatcaacgg gacggagcgc gtcatcgtct cgcagctcca
     1021 ccgctcgccc ggcgtcttct tcacgcgcga gggcgcgcac acgctcatcg gcaagatcat
     1081 tccgtaccgc ggctcgtggg tggaattcga gatcgatccc aaggggctct tcggcgtccg
     1141 gatcgaccgc aagcgcaagt tcccggggac ggtgttcctc cgcgcgctcg gcctggagag
     1201 cgacgagctg atcctgaaga agttctactc cccggtgaag gtctcgttcg agaggggcaa
     1261 ggcgcgcctc gtcctcccgc ccgaggtcct cacgaaggaa gagctccgcg tcaagcatct
     1321 ccgcggcatc cgcaaggacg gccgcatctt cgcggacgtc aagctgaccg ccgagatgaa
     1381 ggcctcgctc gcaaagggcg agagcgtcga ggtgtccgtc gacccggcga agctcggccg
     1441 ggccctcttc gcggccgacg tcgtcgacct cggctcgggc gaggtgctct tcgagacggg
     1501 ctccgagatc cccgaggatc tcgcggcgca gctcgagaac aagcaggcga ccgccgtcga
     1561 ggcgatcttc ccggactggg agcccatcgg cgagacgctc atcgcgaccc tccgcaagga
     1621 cggcgtcaag acgaagcgcg aggcgcttct cgagatctac aagcgcatgc gccccggcga
     1681 cccgccgacg gacgagagcg cgaagaacct cttcgcgggc atgttcttcg acccgcggaa
     1741 gtacgacttc tcgcgcgtcg gccggttcaa gttcaacatc cggaccgggc tccagacgtc
     1801 gctcgaccag aagaccctca cggccgagga cttctacgtc gtgatccagc atctcctcga
     1861 gctcaagcgc gacatcggca agaccgacga catcgacaac ctcggcaacc gccgggtccg
     1921 ctgcgtcggc gagctcctcg agaaccagtt ccggatcggc ctcgtccgca tggagcgcgc
     1981 gatcaagga
//
LOCUS       DAOMFY010000457         1984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_29845_length_1984_cov_8.347849, whole genome shotgun sequence.
ACCESSION   DAOMFY010000457 DAOMFY010000000
VERSION     DAOMFY010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29845_length_1984_cov_8.347849"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLB01_19935"
     CDS             complement(<1..195)
                     /locus_tag="PLB01_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79623.1"
                     /translation="MASLAVLALVLPFAVPPAPKPTLPPVPADVATRMNGLFARATPA
                     VRAWVDAEARRLRPVPQRRPP"
     gene            230..520
                     /locus_tag="PLB01_19940"
     CDS             230..520
                     /locus_tag="PLB01_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79624.1"
                     /translation="MTQRTFPVGAPLPRALSCALEEESAMDLPLKVYVTCPMADLKQV
                     PGTLIAVSPHGFYEVNVVFGANTHLIVVPIDGTTLTAQEPVLSPPASFEVER"
     gene            complement(531..>1984)
                     /gene="ppk1"
                     /locus_tag="PLB01_19945"
     CDS             complement(531..>1984)
                     /gene="ppk1"
                     /locus_tag="PLB01_19945"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQQ79625.1"
                     /translation="IEIQEDEASDLLATIEQEVRRRRFGAVVRLEVAPKTPKRVRKLL
                     VKQLEITENEIYEVDGPLGPGDLMSLVKLDRRDLKDAPFSPAVPAILTQPDTSIFAAI
                     RAGDILLHHPYDSFSPVVEMIERASEDSKTVAIKMTLYRTNPDSGLIPALVRAAENGK
                     QVAVLVELKARFDEVNNITWAKSLEQAGVHVVYGVVGLKTHGKIALVVRREKDEIRRY
                     VHLGTGNYNSSTARTYTDIGLMTCRPEFGEDATRFFNALTAFAGKTTYARLVTAPKDL
                     HKWVLESIARETAHARAGRPSGIRAKMNALVDPKVIAALYEASQAGVPVDLLVRGICC
                     LKPGLPGFSETIRVRSIVGRFLEHSRAFAFENGGEREVWLSSADWMQRNFFRRVEIAF
                     PVVEPALAKQVVEALDTMWKDNVRARELKADGTYVRLAPKEGEAAVDSQTLFVEDARR
                     RVLKAVEAFQNRGAEAFEAAERAAGPEPVESAG"
BASE COUNT          284 a          692 c          705 g          303 t
ORIGIN      
        1 cggcggtcgc cgctggggca cggggcggag cctgcgggcc tcggcgtcca cccacgcgcg
       61 cacggccggg gtcgccctcg cgaagaggcc gttcatgcgc gtcgcgacgt cggcgggcac
      121 gggcggcagc gtcggcttcg gtgccggggg caccgcgaac ggcaggacga gggcgagcac
      181 ggcgagggag gccacggggg aatcgtcccc ccggaggaag gagtccggcg tgacgcagcg
      241 cacgtttccg gtgggcgctc cgctcccgcg cgcgctatcc tgtgcactcg aagaggagag
      301 cgccatggac ctcccgctca aggtctacgt cacgtgcccg atggccgacc tcaagcaggt
      361 gcccgggacg ctcatcgcgg tgtccccgca cgggttctac gaggtaaacg tcgtcttcgg
      421 cgcgaacacg cacctgatcg tcgttccgat cgacggcacg accctcacgg cgcaggaacc
      481 cgtcctcagc ccgcccgcga gcttcgaagt cgagcggtag aagcgtcggc ctagccggcc
      541 gactcgaccg gctcgggtcc cgccgcgcgc tcggcggcct cgaaggcctc ggcgccgcgg
      601 ttctggaacg cctcgacggc cttcagcacg cgccgccgcg cgtcctcgac gaagagggtc
      661 tgcgagtcga ccgcggcctc gccctccttc ggcgcgagac gcacgtacgt cccgtcggcc
      721 ttcagctcgc gcgcgcggac gttgtccttc cacatcgtgt cgagcgcctc gacgacctgt
      781 ttcgcgagcg cgggctcgac gacggggaac gcgatctcga cgcgccggaa gaagttgcgc
      841 tgcatccagt cggccgacga gagccagacc tcccgctccc cgccgttctc gaaggcgaag
      901 gcgcggctgt gctcgaggaa gcggcccacg atcgagcgga cgcggatcgt ctcggagaac
      961 cccggcaggc ccggcttcag gcagcagatc ccccgcacga ggaggtcgac cggcacgccg
     1021 gcctgcgacg cctcgtaaag ggccgcgatg accttcgggt cgacgagcgc gttcatcttc
     1081 gcgcggatgc cggaggggcg ccccgcgcgc gcgtgcgccg tctcccgggc gatggactcg
     1141 agaacccact tgtgcaggtc cttcggcgcc gtcacgaggc gcgcgtacgt cgtcttgccg
     1201 gcgaaggcgg tcagcgcgtt gaagaagcgc gtcgcgtcct cgccgaactc gggcctgcac
     1261 gtcatgagtc cgatgtccgt gtaggtgcgc gccgtcgagg agttgtagtt gcccgtcccg
     1321 aggtggacgt aacgccggat ctcgtccttt tcgcggcgca cgacgagggc gatcttgccg
     1381 tgcgtcttga ggccgacgac gccgtacacg acgtggaccc ccgcctgctc gagcgacttg
     1441 gcccacgtga tgttgttgac ctcgtcgaag cgcgccttca gctccacgag gaccgccacc
     1501 tgcttgccgt tctccgccgc gcggacgagg gccgggatga gccccgagtc ggggttcgtc
     1561 cggtagagcg tcatcttgat cgcgacggtc ttcgagtcct ccgacgcccg ctcgatcatc
     1621 tcgacgaccg gcgagaacga gtcgtacggg tggtgcagga ggatgtcgcc cgcgcggatc
     1681 gcggcgaaaa tagacgtgtc gggctgggtg aggatcgcgg gcaccgcggg cgagaacgga
     1741 gcgtccttga ggtcccggcg gtcgagcttc acgagagaca tcaggtcgcc cgggccgagc
     1801 ggcccgtcca cctcgtagat ctcgttctcc gtgatctcga gctgcttgac gaggagcttg
     1861 cggacccgct tcggcgtctt cggggccacc tcgaggcgca cgacggcccc gaagcggcgg
     1921 cggcggactt cctgctcgat cgtcgcgagg aggtccgacg cctcgtcctc ctggatctcg
     1981 atgt
//
LOCUS       DAOMFY010000458         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_29897_length_1982_cov_5.695900, whole genome shotgun sequence.
ACCESSION   DAOMFY010000458 DAOMFY010000000
VERSION     DAOMFY010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29897_length_1982_cov_5.695900"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PLB01_19950"
     CDS             complement(<1..1046)
                     /locus_tag="PLB01_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQQ79626.1"
                     /translation="MSWLFLSSGLFLGWSLGANDAANLFGTAVGTKMVRFATAALVAS
                     VFVVLGAVSGGAGAAGTLGRLGAVDTLPAAFAVAFAAALSVFLLLKRIGLPVSTSQAI
                     VGAIVGWNLFTGRPTDARTLLQILASWFLCPLLAAGVAAALFLGARAAFPHVKIHLLE
                     VDAWTRAGLLAVGAFGAFSLGQNNIANVMGVFVRVSPFPEAASLGPLRATGVEMLFFL
                     GGLSIATGIYTYSHRVMRRVGEGLTSLSPLAALIVVLAASIVLFLFASPHLERALRSA
                     GLPALPLVPVSSSQAVVGAILGISLVRGSPVKPRPLAQILLGWAAAPLLAMGISFLGL
                     VLLQNVFRLPVTAG"
     gene            complement(1043..>1982)
                     /locus_tag="PLB01_19955"
     CDS             complement(1043..>1982)
                     /locus_tag="PLB01_19955"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQQ79627.1"
                     /translation="RTLGRLGAIDAPAGAFGVALAAGLAVTLLLTRTGLPVSITQAIV
                     GAILGWNLFTGRPTDMKTLGQLLSSWLVSPLVAGTIAAVLFVALRATTSRLKVHLLKE
                     DAFTRASLLAVGAFGAYGLGVNDIANVMGVFVRVSPFPETVSLGPVHITSVQTLFLLG
                     GLAIATGIYTYSERIMRRVGSGLTSLSPLAALIVVLSAGLVLFLFGSQELQGLLKAAG
                     LPGWPLVPVSATQAVVGAILGISLVRRSGIRLSALGEIVTGWVAAPLAALVISLVGLF
                     VLQNVFEMPVSATPVRRPAVAAAAPAPAPVPDAPVR"
BASE COUNT          351 a          671 c          783 g          177 t
ORIGIN      
        1 tcgccggcgg tcacgggcag ccggaagacg ttctggagca ggacgagccc gaggaacgag
       61 atccccatcg cgaggagcgg cgccgccgcc cagccgagga ggatctgcgc gagcgggcgc
      121 ggcttcacgg ggctcccgcg cacgagcgag atcccgagga tcgcgccgac gacggcctga
      181 gagctcgaga cgggcacgag cggcagcgcg ggcagcccgg cggacctcag cgcccgctcg
      241 aggtgcgggg acgcgaagag gaacaggacg atcgacgccg cgaggacgac gatgagcgcc
      301 gcgagcggcg acagcgacgt gaggccctct cccacgcggc gcatgacgcg gtgcgagtac
      361 gtgtagatcc cggtcgcgat cgagaggccg ccgaggaaga agagcatctc gacgcccgtg
      421 gcgcgcagcg gcccgaggga ggccgcctcc gggaacggcg acacgcggac gaagacgccc
      481 atgacgttcg cgatgttgtt ctggccgagg ctgaaagcgc cgaacgcgcc gacggcgagg
      541 agccccgcgc gggtccacgc gtcgacctcg aggagatgga tcttcacgtg cgggaacgcg
      601 gcccgcgccc cgaggaagag cgcggccgcg acgccggccg cgaggagcgg gcagaggaac
      661 cacgacgcca ggatctggag gagcgtccgc gcgtcggtgg gccggcccgt gaagaggttc
      721 cagcccacga tggcgcccac gatcgcctgg gacgtcgaga cggggaggcc gatgcgcttg
      781 aggaggagga agaccgacag cgccgcggcg aacgcgacgg cgaacgcggc cggcagggtg
      841 tccacggcgc cgaggcgccc gagcgtcccc gcggcgcccg ctccgccgga gacggcgccg
      901 agcacgacga acacgctcgc gacgagcgcc gccgtcgcga agcggaccat cttcgtcccg
      961 acggcggtgc cgaagaggtt cgccgcgtcg ttggcgccga gcgaccaccc caggaagagg
     1021 ccgctcgaga ggaacagcca gctcaacgga cgggcgcgtc cggcacggga gcgggcgccg
     1081 gagcggcggc cgccaccgcg ggccggcgca ccggcgtcgc gctgacgggc atctcgaaga
     1141 cgttctggag gacgaagagc ccgacgaggg agatgacgag cgccgcgagc ggcgccgcga
     1201 cccagcccgt cacgatctcg ccgagcgcgc tcaggcggat gccgctccgg cgcacgaggg
     1261 agatcccgag gatggccccg acgacggcct gcgtcgccga gaccggcacg aggggccagc
     1321 cgggcagacc ggccgccttg aggaggccct ggagctcctg cgatccgaag aggaagagga
     1381 cgaggccggc cgagaggacg acgatgagcg ccgcgagcgg cgagagtgac gtgaggcccg
     1441 agccgacgcg ccgcatgatc cgctcggagt acgtgtagat ccccgtcgcg atcgcgagac
     1501 cgccgagcag gaacagcgtc tggacgctcg tgatgtgcac cgggccgagc gacacggtct
     1561 cggggaacgg cgagacgcgc acgaagaccc ccatcacgtt cgcgatgtcg ttgacgccga
     1621 gcccgtacgc gccgaaggcg ccgacggcga ggaggctcgc gcgcgtgaac gcgtcctcct
     1681 tcaggagatg gactttcagc cgggacgtcg tcgcgcgcag cgccacgaag agcacggccg
     1741 cgatcgtccc ggcgacgagc gggctgacga gccacgacga gaggagctgg ccgagcgtct
     1801 tcatgtccgt cgggcggccc gtgaagaggt tccacccgag gatcgcgccg acgattgcct
     1861 gcgtgatcga cacggggagg cccgtccgcg tcaggaggag ggtgacggcg agcccggcgg
     1921 cgagcgcgac gccgaacgcg cccgccggcg cgtcgatcgc gccgaggcgg ccgagcgtgc
     1981 gg
//
LOCUS       DAOMFY010000459         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_29931_length_1981_cov_6.781412, whole genome shotgun sequence.
ACCESSION   DAOMFY010000459 DAOMFY010000000
VERSION     DAOMFY010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29931_length_1981_cov_6.781412"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1270)
                     /locus_tag="PLB01_19960"
     CDS             complement(119..1270)
                     /locus_tag="PLB01_19960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79628.1"
                     /translation="MTVRFGVGLEEVELGGMVGRGGSGEVFAARHRGTGARLAAKVYP
                     FDRMDPEFRPRERFHRECRILSRMEHPAFPALYGCGEEGDGSTGWALMEWIEGAPLSA
                     FRAAPVHEIVRLAYGVASALDALHVQGILHRDIAFDNVLVETRRFGLNPRLIDLGVAK
                     DISSNQDLTMAGAFLGRVPFASPELLTGEGAAGLVDPRSDVFSFGVLLYEWLSGKPPF
                     PGETPERVMKAQRKRAAPKLELRDGAGEADGGLSSFVESLLETDIEKRPASETVVRRL
                     AELRRAYPPPVRELAPSPASVYRAASLGYALLPRRFDAAPALRRLRAVSFPEPPSQPA
                     DAPPRERAAPAGGSPGPGPRPGLGGLVLALGILAFVAACALAAFLVFRR"
     gene            complement(1284..1916)
                     /locus_tag="PLB01_19965"
     CDS             complement(1284..1916)
                     /locus_tag="PLB01_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79629.1"
                     /translation="MTENRPGRPGDVDATASSPAESRSLREMLSDARGRAKGQGVVQG
                     STVFFKNLAESIGHSNRNLKIGLAFLTAAFIVVAVALGSLVLQAIRSQVATKTTLEKQ
                     IQSMDASTRKQIQPVLDAVTSLEKKLLEQQDAQRRELEGVRQLLQADIDAAKQDAAAA
                     RKEAAELRASLKAVEKWADEFGKTYLQDRSNLDTRLRRIEGAPAPAPATK"
BASE COUNT          292 a          708 c          696 g          285 t
ORIGIN      
        1 gaccgcaacg gcgacgcccg tgatcgctcc ggcagccccg gggaaggcgc ccgccgcgcc
       61 gactcccccg ccgactccgc accccgcgcc gacgcccggc ggcgccgcct gacgatcgtc
      121 agcgcctgaa gacgaggaaa gccgccagcg cgcacgcggc cacgaacgcc aggattccga
      181 gggcgagcac cagcccgccg agtcccggtc gcgggccagg cccgggagat ccgcctgcgg
      241 gcgcggccct ctcccgcggc ggcgcgtctg ccggctggga cggcggctcg ggaaacgaga
      301 ccgcacgcag cctgcgaagc gccggcgcgg catcgaaccg cctcggcagg agtgcgtacc
      361 cgagagaggc cgcccggtag acgctggcgg gcgatggcgc aagctcccgg acgggcggag
      421 ggtaggccct gcggagctcg gcgagccgcc tgacgacggt ctcggacgcc ggccttttct
      481 cgatgtcggt ttcgaggagg ctctcgacga acgacgacaa gcctccgtcc gcctccccgg
      541 cgccgtccct cagctccagc ttcggcgcgg ccctctttct ctgcgccttc atgacgcgtt
      601 ccggcgtctc gcccgggaac ggcggctttc ccgagagcca ctcgtagagg aggacgccga
      661 acgagaagac gtcgctgcgg ggatcgacga gccccgcggc gccctccccc gtcagcagtt
      721 cgggggaggc gaacgggacg cgcccgagga acgcccccgc catcgtcagg tcctggttcg
      781 acgagatgtc cttcgcgacg ccgaggtcga tcagccgggg gttgaggccg aagcggcgcg
      841 tctcgacgag gacgttgtcg aatgcgatgt cgcggtggag gattccctgg acgtgcagcg
      901 cgtcgagggc ggaggcgacg ccgtacgcga gacgcacgat ctcgtggacg ggtgcggcgc
      961 ggaaggccga gagaggcgcg ccctcgatcc attccatcaa cgcccatccg gtgctcccgt
     1021 cgccttcctc gccacagccg tagagggccg gaaacgccgg gtgctccatc cgcgagagga
     1081 tccggcactc gcgatgaaag cgctcgcgcg ggcggaactc gggatccatc cggtcgaagg
     1141 gatagacctt cgcggcgagg cgcgccccgg tcccgcgatg gcgcgcggcg aagacctcgc
     1201 ccgagccgcc ccggcccacc atcccaccga gttcgacctc ctcgaggccg actccgaatc
     1261 ggaccgtcac cggccggagg ccctcacttc gtcgcggggg ccggggcggg agcgccctcg
     1321 attcgcctga ggcgcgtgtc gaggttgctg cggtcctgga ggtacgtctt cccgaactcg
     1381 tcggcccatt tctcgacggc cttcagggag gcgcggagct ccgccgcttc cttgcgtgcc
     1441 gcggcggcat cctgctttgc cgcgtcgatg tcggcctgaa ggagctggcg gacgccctcg
     1501 agttcgcgcc tctgcgcgtc ctgctgctcg agcagcttct tctcgaggct cgtcaccgcg
     1561 tcgaggaccg gctggatctg tttgcgcgtc gaggcgtcca tggactggat ctgcttctcg
     1621 agagtcgttt tcgtcgcgac ctggctccgg atggcctgga ggacgagcga gcccagcgcc
     1681 accgcgacga cgatgaacgc ggcggtgaga aaggcgagcc cgatcttgag gttccggttc
     1741 gaatgtccga tcgactccgc gaggttcttg aagaagaccg tcgagccctg aaccacgccc
     1801 tggcccttcg cccggccccg cgcatcggag agcatctccc tcagggacct ggactcggcc
     1861 gggctcgagg cggtggcgtc gacgtccccg ggacgtccgg gtcgattctc ggtcatggcc
     1921 gctccttcat ttcagcacgg agatgaggac ccacaggatg gcaccgaaca gcacgacgat
     1981 g
//
LOCUS       DAOMFY010000460         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_29989_length_1979_cov_7.621102, whole genome shotgun sequence.
ACCESSION   DAOMFY010000460 DAOMFY010000000
VERSION     DAOMFY010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29989_length_1979_cov_7.621102"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLB01_19970"
     CDS             <1..384
                     /locus_tag="PLB01_19970"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQQ79630.1"
                     /translation="ASGRRRRSHSHFASPTRRPPEAAPALSPPRQSPAAAPQRRVRTK
                     PDVEELAALPLSPEQERACRGKVVGVAVEVGEDGSLLSRRVISPVSSTCDALALEVLA
                     RSKFRPAVGEDGRPIEGRFAFVVRF"
     gene            447..1130
                     /locus_tag="PLB01_19975"
     CDS             447..1130
                     /locus_tag="PLB01_19975"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQQ79631.1"
                     /translation="MVPSSSRTAAVVLAASTLFGCAAKTIEMKMAVDVVSEPERASVT
                     YKGKNIGETPKSFEVTTFDDVASITATKADLDVVEKRIRILSPDKAVVVFHLGKGGIS
                     PVAKKLNLTRVLVFEYAEKVCFDSAKADLKPDAIPVLARQAEILNRYFPKAQVYVCGY
                     TDSTGSDAFNDKLSLQRAETVRNFLVNQSVDPARLKTQGFGKQFEVAPNTTAEGRALN
                     RRTEVILPE"
     gene            1154..>1979
                     /locus_tag="PLB01_19980"
     CDS             1154..>1979
                     /locus_tag="PLB01_19980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79632.1"
                     /translation="MHAEEAVSLARQHVGSRYEILEPIGAGGYAAVLKVKNRDNGRLE
                     ALKITMVAPGSDPDAVQRFREETRIAAALDHPSIVRVWASGGEGEVLWYSMELVEGPS
                     LATARGRRFAPGEAARLGEALADALAHSHARGVLHRDVKPENVLLAADGRPKLMDFGI
                     AKSEDSSVRTKTGFLVGSPAYVSPEQLSGTPLDGRTDVYSLGTTLFELLAGRLPFRSR
                     GIEDLARRLDDDAPPLSKYLPEVDTEIERIVRKALARDRRDRYDAAALRDALRAYLA"
BASE COUNT          306 a          717 c          672 g          284 t
ORIGIN      
        1 gcctcgggcc gccggcgccg ctcccacagc cacttcgcga gccccacgcg gcgcccgccc
       61 gaggccgcgc ccgcgctctc gcccccgcgc cagagcccag ccgctgcgcc gcagcggcgg
      121 gtgaggacga aacccgacgt cgaggagctc gcggcgcttc ccctctcgcc cgagcaggaa
      181 cgcgcctgcc ggggaaaggt cgtgggcgtg gccgtcgagg tcggcgagga cgggagcctc
      241 ctctcccgcc gcgtcatctc gccggtttcc tcgacatgcg atgcgctcgc cctcgaggtg
      301 ctcgcccggt cgaagttccg gccggccgtc ggggaggacg ggcggccaat cgagggccgg
      361 ttcgccttcg tcgtgcgctt ctagtcttcc ggacgcccgg cggcatagac tcagtctccg
      421 aaacgcctgt gccgcggagg tgcctcatgg tcccgtcgag ttcgcgtacg gccgccgtcg
      481 tgctcgcggc ctcaacgctc ttcggctgcg ccgcgaagac gatcgagatg aagatggcgg
      541 tggacgtcgt ctccgaaccc gagcgtgcca gcgtgacata caagggcaag aacatcggcg
      601 agacgccgaa gtccttcgag gtgacgacgt tcgacgacgt cgcctcgatc acggccacca
      661 aggcggatct cgacgtcgtc gagaagcgca tccggatcct ctcgcccgac aaggccgtcg
      721 tcgtctttca cctcgggaaa ggcggaatct cgcccgtcgc gaagaagctc aacctcaccc
      781 gcgtcctcgt gttcgagtac gcggagaagg tctgtttcga ctccgccaag gccgacctca
      841 agcccgacgc gattccggtc ctggcgcggc aggccgagat cctcaatcgc tacttcccga
      901 aggcccaggt gtacgtctgc ggctacaccg actcgacggg gagcgacgcc ttcaacgaca
      961 agctctcgct ccagagggcc gagacggtcc gcaacttcct cgtgaaccag agcgtggatc
     1021 cggcccgcct gaagacgcag gggttcggaa agcagttcga ggtcgcgccg aacaccacgg
     1081 ccgagggccg ggccctgaac cggcgcaccg aagtcatcct tccggaatga cggggcccgg
     1141 ataggatccc cgggtgcacg ccgaggaagc cgtctcactc gccaggcagc acgtcgggag
     1201 ccgatacgag atcctcgagc cgatcggcgc gggcgggtac gcggccgtcc tgaaggtgaa
     1261 gaaccgcgac aacgggcggc tcgaggcgct gaagatcacg atggtcgccc cgggttcgga
     1321 tccggacgcg gtgcagcgct tccgcgagga gacgcggatt gcggcggcac tcgaccaccc
     1381 gtcgatcgtg agggtctggg cctcgggcgg cgagggcgag gtgctctggt attcgatgga
     1441 gctcgtcgag ggacccagcc tggccaccgc gcggggacgc cgcttcgctc ccggcgaggc
     1501 cgcgcggctt ggcgaggcgc ttgcggacgc cctcgcccac agccacgcga ggggcgtgct
     1561 ccaccgcgac gtcaagcccg agaacgtcct tctcgccgcg gacggccggc cgaagctcat
     1621 ggacttcggg atcgccaagt ccgaggacag ctcggtccgg acgaagacgg ggtttctcgt
     1681 cggctcgccc gcctacgtct cgccggagca gctctcgggg acgcctctcg acggtcgcac
     1741 ggacgtctac tcgctcggga cgaccctgtt cgagctcctc gccggccggc tcccgttccg
     1801 ctctcgcggc atcgaggatc tcgcgcgccg gctggacgac gacgctccgc ccctctcgaa
     1861 ataccttccc gaggtggaca ccgagatcga gcgcatcgtc cgcaaggccc tcgcccgcga
     1921 ccggcgcgac cgctacgacg cggccgccct gcgcgacgcg ctgcgggcgt acctcgcgc
//
LOCUS       DAOMFY010000461         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30190_length_1972_cov_5.839332, whole genome shotgun sequence.
ACCESSION   DAOMFY010000461 DAOMFY010000000
VERSION     DAOMFY010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30190_length_1972_cov_5.839332"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLB01_19985"
     CDS             <1..1029
                     /locus_tag="PLB01_19985"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HQQ79633.1"
                     /translation="GPRLGVPSVIACRAALGLRGGALVSLILYVTNFAWIAVNNVIAA
                     SACARVLGGPASVRYWAFGLGLLATAVVAGGPRAVRHADRAAVPLLLLVGAVLTFAFW
                     RLPSAAAAAPGNGSMTAVRGLDVVIGYQVSWLLVFGDYPRYTRSPGKAAAAVFLGLAL
                     TSLWFVPLGFVAARAAGSTNPGAMIAAAGFGALGALLLALATLTTNFVNIYMSALAWK
                     SLVPAAKDRASVWTIGVVGAILSLFSGWLDRYADFMLLLGGLLVPVGGVLLAHFFLTK
                     KPVSVPDLYDEKGPASRHGGFAIPGMCGWAAGAAAYVLAGSTGGTLPALATALVVTWG
                     LERVLRAT"
     gene            1049..>1972
                     /gene="dinB"
                     /locus_tag="PLB01_19990"
     CDS             1049..>1972
                     /gene="dinB"
                     /locus_tag="PLB01_19990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQQ79634.1"
                     /translation="MAAPPRRILHLDMDAFYTSVEQRDDPALRGRPVAVGGDPKGRGV
                     VAAASYEARKFGVRSAIPMARAVRLCPALVIVKPDFRKYSAVSKEIFALYRAVTPLVE
                     PLSLDEAYLDVTENAWGEPLGMEVARRLKAEVREATRLTVSAGVAPNKFLAKIASGWK
                     KPDGLTVIAPERMEAFLAKLPVDALWGVGPKTAERLRAIGCAKLVDVRAADPAALRRV
                     VGSWAESLVRLAHGDDARPVVPDRTRKSVSTEDTFREDLTDTREMRTIVESMARHDAS
                     FLAEKRLLARTVTLKVRYADFTTVTRSDSARP"
BASE COUNT          223 a          736 c          706 g          307 t
ORIGIN      
        1 gggccgcgtc tcggcgtccc gtccgtcatc gcctgccgcg cggcgctcgg gctgcgcgga
       61 ggggcgctcg tctcgctgat cctctacgtc acgaacttcg cgtggatcgc cgtgaacaac
      121 gtgatcgcgg cgtccgcgtg tgcgcgcgtc ctcggcggac ccgcgagcgt tcggtactgg
      181 gcgttcggcc tcggcctcct cgcgaccgcg gtcgtcgcgg gcgggccgcg ggccgtccgt
      241 catgcggacc gcgcggcggt gccgctcctc ctcctcgtcg gcgccgtcct cacgttcgcg
      301 ttctggcggc tcccctcggc cgccgcggcg gcgcccggga acggctcgat gacggcggtg
      361 cggggcctcg acgtcgtgat cggctaccag gtctcgtggc tcctcgtctt cggggactac
      421 ccgcgctaca cgcgctcgcc ggggaaggcc gcggcggccg tcttcctcgg gctcgcgctg
      481 acgagcctgt ggttcgtgcc cctcgggttc gtcgccgcgc gggcggcggg ctcgacgaac
      541 cccggcgcga tgatcgcggc cgcgggcttc ggcgccctcg gcgccctcct cctcgccctc
      601 gcgacgctca cgacgaactt cgtgaacatc tacatgtcgg cgctcgcgtg gaagagcctc
      661 gttccggccg cgaaggaccg cgcgtccgtg tggacgatcg gcgtcgtcgg ggcgatcctc
      721 tcgctgttct cgggctggct cgaccgctat gcggacttca tgctcctcct cggcggtctt
      781 ctcgttcccg tcggcggcgt cctcctcgcc cacttcttcc tgaccaagaa gcccgtctcg
      841 gtgccggacc tgtacgacga gaaggggccg gcgagccgcc atggcggctt cgcgatcccg
      901 gggatgtgcg gatgggccgc cggcgcggcc gcgtacgtcc tcgccggctc gaccggcgga
      961 acgctgcccg cgctggcgac ggcgctcgtc gtgacctggg ggctcgagcg cgtcctccgc
     1021 gccacctgag cggcgcagaa tcggccgcgt ggccgcacct ccccgccgca tcctccacct
     1081 cgacatggac gccttctaca cgtccgtcga gcagcgggac gatcctgcgc tcaggggcag
     1141 gcccgtcgcc gtgggcggcg acccgaaggg ccgcggcgtc gtcgccgccg cgagctacga
     1201 ggcgcggaag ttcggcgtgc gctccgcgat cccgatggcg cgcgcggtgc gcctgtgtcc
     1261 tgcgctcgtg atcgtcaagc ccgacttccg gaagtactcc gccgtgtcga aggagatctt
     1321 cgcgctctac cgcgcggtga caccgctcgt cgagcccctc tccctcgacg aggcctacct
     1381 cgacgtcacc gagaacgcgt ggggcgagcc gctcgggatg gaggtcgcga ggcgcctcaa
     1441 ggccgaggtg cgggaggcga cgcgcctgac ggtgtcggcc ggcgtcgcgc cgaacaagtt
     1501 cctcgcgaag atcgcctcgg gatggaagaa gcccgacggg ctgaccgtga tcgcgccgga
     1561 gcgcatggag gccttcctcg cgaagctccc cgtggacgcc ctctggggcg tcgggccgaa
     1621 gaccgccgag cggctgcggg ccatcggctg cgcgaagctc gtggacgtcc gagccgcgga
     1681 tcctgccgcg ctccgccgcg tcgtggggag ctgggcggag tctctcgtcc gcctcgcgca
     1741 cggcgacgac gcgcgcccgg tcgttcccga ccgcacccgg aagtccgtca gcacggagga
     1801 cacgttccgc gaggacctga cggacacgcg cgagatgcgg acgatcgtcg agtcgatggc
     1861 gcgccacgat gcttcgttcc tcgccgagaa gcggctcctc gcgcgcaccg tcacgctcaa
     1921 ggtgcgctac gcggacttca cgaccgtgac gcgcagcgac agcgcgcggc cg
//
LOCUS       DAOMFY010000462         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30239_length_1970_cov_7.140470, whole genome shotgun sequence.
ACCESSION   DAOMFY010000462 DAOMFY010000000
VERSION     DAOMFY010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30239_length_1970_cov_7.140470"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..535
                     /locus_tag="PLB01_19995"
     CDS             125..535
                     /locus_tag="PLB01_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79635.1"
                     /translation="MRAGPVAAARVALAAALVLAGCGSIETPTQPTACVPLTGQYTGS
                     FTDSCGARTLDVTLFQTGCKVVADLPGVGSLTGNVSGATLVFAIGFSPCGGSASGTMT
                     LGVDGGLTGTYTGQATGGGACCGGVSGSVVLVRK"
     gene            complement(538..1155)
                     /locus_tag="PLB01_20000"
     CDS             complement(538..1155)
                     /locus_tag="PLB01_20000"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQQ79636.1"
                     /translation="MTENRRDEDRRHLLEELAAVESLEGRALPEGDELLKLDPEWVSL
                     RRRFDEAFAAIGLAAVVPPPASLKARVLAGLAGVAPAPALPPGGRFVSQGIAAVIVAD
                     APWVSSPLPGIEYKLLHRDEDRRSTTRLLRFAAGATYPSHRHGGVEEVFVLDGSVTLN
                     GILLRPGDYCRAEAGTAEPTAYSEAGGMAIIVSSDLDAFDAAPAG"
     gene            complement(1152..1748)
                     /locus_tag="PLB01_20005"
     CDS             complement(1152..1748)
                     /locus_tag="PLB01_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ79637.1"
                     /translation="MTVPPQRSPEALPEAAELLARIALRDAAALSSLYDLIGSAVHGV
                     CRRILRDPADAEEASMDVFLQVWEKASRFDPERGDALTWLLTLARSRAIDRLRSREAA
                     RRHETGLDEGHDAPSSDPGPETQSSLAQRAALVRGALADLPAEQREVLELAYFEGLTH
                     SEIAERLSQPLGTAKSRIRLALVHLRRTLEPRTEGGIA"
BASE COUNT          270 a          661 c          763 g          276 t
ORIGIN      
        1 gcctccttcg cctctgtcgc ggcctcgacc gaatcctcct cgagacgggc gggagcgcct
       61 accgcgcgaa ggactgcgtc ggcaccgtcg accggctcgc ggcggcgcgc gcgggaaagg
      121 ccgcgtgaga gcgggcccgg tcgccgccgc gcgggtcgcg ctcgcggctg ctctcgttct
      181 tgcgggctgc ggcagcatcg agaccccgac tcagccgacg gcctgcgtgc cgctcacggg
      241 gcagtacacg ggctcgttca cggactcgtg cggcgcccgg acgctcgacg tgacgctctt
      301 ccagacgggc tgcaaggtgg tcgcggacct gccgggcgtc ggctcgctca cggggaacgt
      361 gagcggagcg acgctcgtct tcgcgatcgg gttctcgccg tgcggaggct cggcgagcgg
      421 cacgatgacg ctcggcgtgg acggcggcct gacgggcacc tacacgggcc aggcgacggg
      481 cggcggcgcc tgctgcggcg gggtgagcgg gtcggtcgtt ctcgtccgga agtagggtca
      541 gcccgcgggc gccgcgtcga aagcgtcgag gtcggacgag acgatgatcg ccatcccgcc
      601 tgcttcgctg taggccgtcg gctcggctgt tccagcctcg gcccggcagt agtcgccggg
      661 cctgaggagg atcccgttca acgtgacgct gccgtcgagg acgaagacct cctcgacgcc
      721 tccgtggcgg tgcgagggat acgtcgctcc cgccgcgaag cgcaggagcc gggttgtcga
      781 gcggcgatct tcgtcgcggt gcaggagctt gtattcgatg ccgggaaggg gcgaggagac
      841 ccagggcgcg tccgccacga tcacggccgc gatgccctgc gatacgaaac ggccgcccgg
      901 cggcagggcc ggagccggcg cgacgcccgc gagccctgcg agaacgcgcg ctttgagcga
      961 cgcgggaggc ggcacgacgg ccgcgaggcc gattgcggca aaggcctcgt cgaaccggcg
     1021 gcggagcgag acccattcgg ggtccagctt cagaagctcg tcgccctcgg gcaacgcccg
     1081 cccctccagc gattcgacgg ccgcgagctc ctcgaggagg tgacggcggt cttcgtcgcg
     1141 gcgattttcc gtcacgcgat gcctccttcg gtccggggct cgagcgtgcg ccggagatgc
     1201 acgagggcga gccgaattcg cgattttgcc gtacccaggg gctgagagag gcgctcggcg
     1261 atctcggagt gcgtgagccc ttcgaagtac gccagctcca ggacctctcg ctgctcggca
     1321 ggcagatcgg ccagcgcgcc cctcacgagt gccgcgcgct gggcgagcga ggattgcgtt
     1381 tccgggcccg gatcggacga gggcgcgtcg tggccttcgt cgaggccggt ctcgtggcgc
     1441 cgcgccgctt cgcgcgatcg caggcggtcg atcgcgcgcg accgcgcgag agtgaggagc
     1501 caggtcaggg cgtctccgcg ctccgggtcg aagcgggagg ccttctccca gacctggagg
     1561 aatacgtcca tcgacgcttc ctcggcgtcg gccgggtccc ggaggatgcg ccggcagacg
     1621 ccgtggacgg cgcttccaat gaggtcgtag agcgaggaca gggcggccgc gtcgcgcaga
     1681 gcaatccggg cgagaagctc cgccgcttcg gggagcgcct ccggcgaacg ctggggcgga
     1741 acggtcaacg aactgcccgc atggaggccg ggattatctc cgaaggtcgg gcgcaggacg
     1801 ggtgccaagg ctcgggccac gccggaggta cggaggccgg gaccgcgtgg atcaccggcc
     1861 ccggagcgtc tgcatcatgg ggtgaagggg agaggcgcgt agaatggcgc ggatgcgacg
     1921 ggttctcctt ctcgccgtgc tcgccgccgc cctcgtggcg gcgccggccg
//
LOCUS       DAOMFY010000463         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30426_length_1963_cov_6.095388, whole genome shotgun sequence.
ACCESSION   DAOMFY010000463 DAOMFY010000000
VERSION     DAOMFY010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30426_length_1963_cov_6.095388"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLB01_20010"
     CDS             complement(<1..838)
                     /locus_tag="PLB01_20010"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQQ79638.1"
                     /translation="MRPAPFLLVAALAVPSLRAPAAEPAKPAAVHVPFVEGKPFADVL
                     KRAKAEGKPVMLDVVAAWCGPCKIMDKTTFADAGVVAWAKKSVVPARVDAEKGEGRKI
                     SARYQAFSFPTVVFLDGDGNEIDRLVGGYQAADFLRGGEAVLGGKTPLLEGLAKLRAK
                     WDHEEGIRLGGALALRRDRVRLRPIALRVVSEEGELSRPEVFQLFAQLVAIETLDDDF
                     SPETGDLIATFLPRLGNDPRRGFFAAAYVTALGKRGDVATARSVAAETLAAVGEGGPY
                     NAD"
     gene            complement(853..1656)
                     /gene="truA"
                     /locus_tag="PLB01_20015"
     CDS             complement(853..1656)
                     /gene="truA"
                     /locus_tag="PLB01_20015"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQQ79639.1"
                     /translation="MTALQVLRATLEYDGGRYRGWQSQVNARTVQGELYAACRDVLGK
                     DVKVNGAGRTDAGVHAFAQVASLHGPFTAPASELGRIRQALNDRLPPDIHVLNLQPAP
                     RTFHARHDAAERVYRYRISRRRTAFGKRYVWWIKDRLDAKAMQAAADLFPGRHDFGSF
                     CENAEGHDSTLVEVGFARLETPPEAPDLLIFRIGASHFLWKMVRRLTGTLVEVGTGRL
                     GVKDVKEMLATRSAKSAAWTSPPSGLFLESVTYPGDPPPPPIDPFRPGF"
     gene            1692..>1963
                     /locus_tag="PLB01_20020"
     CDS             1692..>1963
                     /locus_tag="PLB01_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79640.1"
                     /translation="MLVTSETLEDARPFTRARDAGAEARAAASLRALLSALKDAAGDA
                     WAGAALGANPIQLLDRVEVVSPTAVLDMSFKDPGQTPQFDYPPGATG"
BASE COUNT          269 a          682 c          746 g          266 t
ORIGIN      
        1 ggtcggcgtt gtagggcccg ccctcgccga cggccgcgag cgtctcggcg gcgacggagc
       61 gggccgtcgc gacgtcgccg cgcttgccga gcgccgtgac gtaggccgcc gcgaagaagc
      121 cgcgccgcgg gtcgttgccg aggcgcggga ggaaggtcgc gatcaggtcg ccggtttcgg
      181 gggagaagtc gtcgtcgagc gtctcgatgg cgacgagctg cgcgaagagc tggaagacct
      241 ccgggcgcga gagctcgccc tcctccgaga cgacgcggag cgcgatgggg cgcagccgca
      301 cacggtcgcg gcggagcgcg agggcgcctc cgaggcggat gccctcctcg tggtcccact
      361 tcgcccggag cttcgcgagg ccttcgagga gcggcgtctt cccgccgagg acggcctcgc
      421 cgccgcgcag gaagtcggcg gcctggtagc cgccgacgag ccggtcgatc tcgttcccgt
      481 cgccgtcgag gaagacgacc gtcgggaagg agaaggcctg gtagcgggcg gagatcttcc
      541 ggccctcgcc cttctcggcg tcgacgcggg cggggacgac ggacttcttc gcccacgcga
      601 cgacgcccgc gtcggcgaac gtcgtcttgt ccatgatctt gcacgggccg caccacgcgg
      661 cgacgacgtc gagcatgacg ggcttgcctt cggccttcgc ccgcttcagg acgtcggcga
      721 acggcttgcc ttcgacgaac gggacgtgga cggcggcggg tttcgcgggc tcggcggcgg
      781 gagcccggag ggaaggaacg gcgagcgcgg cgacgaggag gaacggggca ggtctcaccc
      841 gcggattctg cctcagaagc cggggcggaa ggggtcgatc ggcggcggcg gaggatcccc
      901 cgggtacgtg acggactcga ggaagagccc ggacggcggc gacgtccacg cggcgctctt
      961 ggccgagcgc gtcgcgagca tctccttcac gtccttgacg ccgagccgtc ccgtcccgac
     1021 ctccacgaga gtccccgtca ggcggcgcac catcttccag aggaagtgcg acgcgccgat
     1081 ccggaagatc aggaggtcgg gcgcctcggg cggcgtctcg aggcgggcga acccgacctc
     1141 gacgagcgtc gagtcgtgcc cctcggcgtt ctcgcagaag ctcccgaagt cgtggcggcc
     1201 cgggaacagg tcggcggcgg cctgcatcgc cttcgcgtcg aggcggtcct tgatccacca
     1261 gacgtagcgc ttgccgaacg ccgtgcgccg ccgcgagatg cggtagcggt agacgcgttc
     1321 ggcggcgtcg tggcgcgcgt ggaacgtgcg cggggccggc tggaggttca ggacgtggat
     1381 gtccggcggg aggcggtcgt tgagcgcctg ccggatcctc ccgagctccg acgcgggcgc
     1441 cgtgaacgga ccgtgcagcg aagcgacctg cgcgaacgcg tggacgccgg cgtccgtgcg
     1501 gccggcgccg ttcaccttga cgtccttgcc gaggacgtcg cggcaggccg cgtagagctc
     1561 tccctgcacc gtgcgggcgt tcacctgcga ctgccagccc cggtaacgcc cgccgtcgta
     1621 ctcgagggtc gcgcggagga cctggagggc ggtcacgccc ccattgtcgc ctcgaacccg
     1681 ttatcctgcc cgtgctcgtg acgagcgaga cgctggaaga cgcccgcccc ttcacgcgtg
     1741 cccgcgacgc cggcgccgaa gcgcgcgcgg ccgcgagcct gagggcgctt ctctccgccc
     1801 tgaaggacgc cgccggagac gcgtgggccg gggccgcgct cggcgccaat cccatccagt
     1861 tgctcgaccg cgtcgaagtc gtcagtccga cggcggtcct cgacatgtcc ttcaaggatc
     1921 ccggccagac gccgcagttc gactatccgc cgggcgcgac ggg
//
LOCUS       DAOMFY010000464         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30427_length_1963_cov_5.581761, whole genome shotgun sequence.
ACCESSION   DAOMFY010000464 DAOMFY010000000
VERSION     DAOMFY010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30427_length_1963_cov_5.581761"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..1040
                     /locus_tag="PLB01_20025"
     CDS             231..1040
                     /locus_tag="PLB01_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79641.1"
                     /translation="MTRFPEPTPAPGGAAVFADSHLGQVEGDADDLLASLESVRQRGF
                     SAAVLLGDIFHYFIGHPKLETPFLRRTLEGFAAVAEQGLQLRYVEGNRDFFVAGTPYA
                     SPFAAYGLTDGLKVGEATYAFVHGDRVNTADLPYRFWRTISKNPVARAALEVIPGGLA
                     RGIVSRTEARLYRSNFRHKKRLPEEALRAEGRQARAQGYDALLVGHFHVGRTFAAADG
                     VTHVLPAWLEERKHAEIAPDGRLAIVEEPTAERARRRGFGALEADPASPPR"
     gene            1037..>1963
                     /locus_tag="PLB01_20030"
     CDS             1037..>1963
                     /locus_tag="PLB01_20030"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79642.1"
                     /translation="MSAAPLLRGRLSGALRVPGSKSVTNRALLAAACAPGESLLLNPL
                     ESSDTRALAAALRLLGAEIFSEGVGWRVRGPLRPRGDSAPELVVLVGDAGTPARILAA
                     LLAAVPGRFVLDGSPRMRERPMSPLFEAIRTLGGEIRCLAREGFLPAAIRGGTLHGGR
                     VAIRGDVSSQFLSALQLVSPLVPGGVTLDVTGPVVSGAYLALTRRVLEGFGASPGAGY
                     RPARFRVPGDDSAACFPIAGALVSGGRVTVRGLARDSEQPDAEFRAWAVRAGGVLAWE
                     TSAGGEESLVAAGPPGGARVLRPMDVDVDAAPD"
BASE COUNT          237 a          716 c          712 g          298 t
ORIGIN      
        1 cgaacggcgt cggggcggaa cgtgacgagg accgggacac gcgtcccgcc cgcgagcgcg
       61 cgccccacgt ccggcgacac gtccgcggcc cgcgcggcgg tcgacgcgac gatcagcgcg
      121 gcagccgccg cgaggcgagc aagcgagggg gagttcatgg aaggccgtgc accgcagacc
      181 gtcgaagcaa ctcgcgtgcc tgtagtgcct tcagccgtta agatctcacc gtgacgcgtt
      241 tccccgagcc gacgccggcc ccaggcggcg cagccgtgtt tgcggactcg catctcggcc
      301 aggtagaagg agatgccgac gacctgctcg cgtctctcga gtccgtccgg cagcgcggtt
      361 tctcggcggc ggtgctgctc ggcgacatct ttcattactt catcgggcac ccgaagctcg
      421 agacgccgtt cctgcggcgc acgctggagg gcttcgccgc ggtcgccgag cagggacttc
      481 agctgcgcta cgtcgagggg aaccgcgact ttttcgtcgc cggaacaccc tacgcctccc
      541 cgttcgcggc ctacggcctg acggacgggc tcaaggtcgg ggaggcgacg tacgcgttcg
      601 tccacggcga tcgcgtgaac acggccgacc tcccgtaccg cttctggcgc acgatctcga
      661 agaatcccgt cgcgcgcgcg gcgctcgagg tgatcccggg cgggctcgcg cgcggaatcg
      721 tgtcgcggac cgaggcgcgg ctctaccgat cgaatttccg ccacaagaag cggctccccg
      781 aagaggcgct tcgcgccgag ggccggcagg cgcgcgcgca gggatacgac gcgctcctcg
      841 tcggccactt ccacgtcggg cgcacgttcg ccgcggccga cggcgtcacg cacgtgctgc
      901 ccgcgtggct cgaggagcgc aagcacgccg agatcgcgcc ggacgggcgg ctggcgatcg
      961 tcgaggaacc gaccgccgag cgcgcgcgcc gccgcgggtt cggggccctg gaggcggacc
     1021 ccgcctcccc gccgcgttga gcgccgcccc tctccttcgc ggccggctct cgggcgcgct
     1081 ccgcgtcccc ggcagcaaga gcgtcacgaa ccgcgcgctc ctcgcggcgg cctgcgcccc
     1141 gggagagagt ctgctgctga atcccctgga gagttcggac acccgcgcgc ttgcggccgc
     1201 ccttcggctt ctcggagccg agattttctc tgaaggtgtg ggttggaggg tgcgcggtcc
     1261 gctgcgtccc cgcggtgact cggcgccgga gttggtcgtt ctcgtcggag atgcggggac
     1321 gcccgcgcgc attctcgccg cgctgctcgc cgcggtgccc ggccgcttcg ttctcgacgg
     1381 cagccctcgc atgcgagaac ggccgatgtc ccctctattc gaagcaatcc gaaccctcgg
     1441 cggcgaaatc cgatgccttg ctcgtgaggg atttcttccc gcggcgatcc gcggcgggac
     1501 gcttcacggc gggcgcgtgg ccatccgcgg agacgtgtcg tcgcagttcc tctcggcgct
     1561 gcagctcgtt tctccgctcg tcccgggcgg cgtgacgctc gacgtgacgg ggcccgtcgt
     1621 ttcgggcgct tacctcgcgc tgacgcgccg ggtcctcgag gggttcggcg cctctcccgg
     1681 cgccggctat cgcccggcgc gctttcgcgt gcccggcgac gactcggcgg cgtgcttccc
     1741 gatcgcggga gcgctcgtgt cgggcggacg cgtgacggtg cgcggccttg cccgagactc
     1801 cgagcagccc gacgccgagt tccgcgcgtg ggccgtcagg gccggcggcg tgctcgcgtg
     1861 ggagacgtcc gccgggggcg aggagtcgct ggtcgcggcc ggccccccgg gcggggcccg
     1921 cgtattgagg cccatggacg tcgacgtcga cgcggctccc gac
//
LOCUS       DAOMFY010000465         1962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30458_length_1962_cov_5.722077, whole genome shotgun sequence.
ACCESSION   DAOMFY010000465 DAOMFY010000000
VERSION     DAOMFY010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30458_length_1962_cov_5.722077"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /gene="gcvPB"
                     /locus_tag="PLB01_20035"
     CDS             <1..1462
                     /gene="gcvPB"
                     /locus_tag="PLB01_20035"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQQ79643.1"
                     /translation="FGSLFDATRAGRTGVHLPALDVPAADPASLYGDGHRPEVAGEIE
                     ASEVDVVRHFTRLSQMNFAIDTALYSLGSCTMKHNPRINEEIARLAGFNDTHPYAPEH
                     LAQGNLELMWRLEQALKALTGFARVTLQPSAGANGELTGALMIRAAHVKAGNPRKNIL
                     VPDSAHGTNPATAHFAGYDVVELTSNAHGTLDLAELASKLDEDVAALMITVPNTLGVF
                     EERILDIAKMVHDKGAYLYLDGANFNAFVGKVRPADMGVDVMHMNLHKTFSTPHGGGG
                     PGAGPVGVAPALVPFLPVPTVENEGGVFRLDVDRPDTIGRMRTFYGNFGVLVRALTYI
                     VSYGNRIHEVAENAVLNANYIRKKLEPFYHLKYDAPSYHEVVFDDKKQAAFGLHNIDV
                     AKRLMDYGFHPPTMSFPLIVPGALMIEPTESEPKEELDAFIDAMIAIAKECESAPEIV
                     HGAPHTTPVRRLDEAGAAREASRPGSFAPVLRAPVR"
     gene            1639..>1962
                     /locus_tag="PLB01_20040"
     CDS             1639..>1962
                     /locus_tag="PLB01_20040"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HQQ79644.1"
                     /translation="MPRVRVAVLDGRRLARALAAGARAVSLHREAIDRINVFPVADRD
                     TGSNLAATLDRVSAAVAGTPERSLAAVARRAADEALLGARGNSGAIVAQALEGFAEAV
                     RGDARL"
BASE COUNT          323 a          710 c          644 g          285 t
ORIGIN      
        1 cttcggctcg ctcttcgacg ccacccgcgc cggccgcacc ggcgtccacc tgcccgcgct
       61 ggacgtaccc gcggccgacc cggcctcgct ctacggcgac gggcaccgcc ccgaggtcgc
      121 gggcgagatc gaggcgagcg aggtggacgt cgtccgccac ttcacgcgcc tctcgcagat
      181 gaacttcgcg atcgacacgg ccctctattc cctcggctcg tgcacgatga agcacaaccc
      241 gcgcatcaac gaggagatcg cgcgcctcgc ggggttcaac gacacccatc cctacgcgcc
      301 cgagcacctc gcgcagggga acctcgagct catgtggcgc ctcgagcagg cgctcaaggc
      361 gctcacgggg ttcgcgcggg tcacgctcca gccctcggcc ggcgcgaacg gcgagctgac
      421 cggagcgctc atgatccggg cggcgcacgt caaggccggg aacccgcgca agaacatcct
      481 cgtcccggac tcggcgcacg ggacgaaccc ggcgaccgcc cacttcgcgg gttacgacgt
      541 cgtcgagctc acgtcgaacg cccacggcac gctggacctc gcggagctcg cctcgaagct
      601 ggacgaggac gtcgcggcgc tcatgatcac ggtcccgaac acgctcggcg tcttcgagga
      661 gcggatcctg gacatcgcga agatggtcca cgacaagggc gcgtacctct acctcgacgg
      721 cgcgaacttc aacgcgttcg tcggcaaggt gcggccggcg gacatgggcg tcgacgtcat
      781 gcacatgaac ctgcacaaga cgttctcgac tccgcacggc gggggaggcc cgggcgcggg
      841 acccgtcggc gtcgcgccgg cgctcgtccc gttcctcccg gtcccgaccg tcgagaacga
      901 gggcggcgtc ttccgcctcg acgtcgaccg cccggacacg atcgggagga tgcgcacgtt
      961 ctacgggaac ttcggcgtcc tcgtccgcgc gctcacgtac atcgtgtcgt acggaaaccg
     1021 catccacgag gtggccgaga acgccgtcct gaacgcgaac tacatccgga agaagctcga
     1081 gccgttctac cacctcaagt acgacgcgcc gagctatcac gaggtcgtgt tcgacgacaa
     1141 gaagcaggcc gcgttcgggc tccacaacat cgacgtcgcg aagaggctca tggactacgg
     1201 cttccacccg ccgacgatgt ccttcccgct catcgtgccg ggcgccctca tgatcgagcc
     1261 gacggagtcc gagccgaagg aagagctcga cgcgttcatc gacgcgatga tcgcgatcgc
     1321 aaaggagtgc gagtccgccc cggagatcgt ccacggcgcc ccgcacacga cgcccgtgcg
     1381 ccggctcgac gaggcgggcg ccgcccgcga ggcgagccgg ccgggcagct tcgctcccgt
     1441 cctccgagcg cccgttcgct gaaagcagag agagggagag agaaagattt cttcgaaagt
     1501 gacgagggga gggaatacgt cgcgtctgcg cggccacccc tcgcgtggag cgccgtggcg
     1561 cccgcgttct tgaccttcta agaaaatctt ctcttctctc ttttctccgg attttccgaa
     1621 ccaaatagaa ttctccgaat gcctcgtgtc cgcgtcgcgg tcctggacgg gcggcgcctt
     1681 gcgcgcgccc tcgcggcggg agcgcgcgcg gtctccctgc accgcgaggc gatcgaccgc
     1741 atcaacgtct ttccggtcgc cgaccgcgac acgggctcga atctcgccgc gacgctggac
     1801 cgcgtttctg cggcagtcgc cggcacgccc gagaggagcc tcgccgccgt cgcacgccgg
     1861 gcggcggacg aggcgctcct cggggcgcgc ggcaactcgg gggcgatcgt ggcgcaggcg
     1921 ctcgagggtt tcgcggaggc cgtccgcgga gacgcccgtc tt
//
LOCUS       DAOMFY010000466         1948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30860_length_1948_cov_6.634443, whole genome shotgun sequence.
ACCESSION   DAOMFY010000466 DAOMFY010000000
VERSION     DAOMFY010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30860_length_1948_cov_6.634443"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PLB01_20045"
     CDS             <1..1358
                     /locus_tag="PLB01_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79645.1"
                     /translation="DASGPYFDDLALGVLPLLFAAAAWRDPRGRAALLVAGGAAVLSF
                     GRFLPGYGAVASALSSVRYPEKWWLLATFALAAAAAVGVDAVFFGERDVRESARRVLL
                     RTAGVLALLCGTLLGLCIGAEDFLRKVLWVVGLGAGDASGAAVAQVLRMPLLTATATL
                     VVCTAAFRSASPLFTFKENLRHPLLLSTLVALLFLGDAARRVAGTCPGGPPDLYRKET
                     PEVALVRAQAGDGRFFDDGADDRATVVRRTLEAGGLDLLRPATGVVFGIRYLGDNDVD
                     RLTAAPAVRWTAALAAMPWGEEKVRILRAAGVALVRTASPGPDPAATEEIGRWGSDRF
                     VRIAGARPEFALLPEGEGGSVLVVERRPARVVLRADVTLPSATLWIGRTFDPNWRAHA
                     DGREIALTPADGWRMEAKLPKGVHDVTLRYANPLFGAGLAVSLAALAAAAALLRAGRP
                     A"
     gene            1355..>1948
                     /locus_tag="PLB01_20050"
     CDS             1355..>1948
                     /locus_tag="PLB01_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79646.1"
                     /translation="MRLRRAALLLGAILLAWVPYRAFFGSSVPVGRDLLFYFYPLKAH
                     LAEGLSRGELPWVDRFRWGGAPLLGGPSAAPFDPANLLFLALPLGAAMKAWMLLHLAL
                     LVAGFAAFARRLGLAAAPAALSGLVFALAGTTVSVVPFPPTLSALSVLPWFAAFVLDT
                     VRNPSARSAAKTAAAAALVAVASAPEFLLYGAAVAAML"
BASE COUNT          179 a          719 c          713 g          337 t
ORIGIN      
        1 tcgacgcctc gggcccgtac ttcgacgacc tcgcgctggg cgtcctgccg ctcctcttcg
       61 cggcggccgc gtggcgcgac ccgcgcgggc gcgcggcgct cctcgtcgcg ggcggcgcgg
      121 ccgtcctgtc gttcggccgc ttcctcccgg gctacggcgc ggtcgcctcc gcgctttcgt
      181 ccgtgcgcta cccggagaag tggtggctcc tcgcgacgtt cgcgctcgcg gctgcggcgg
      241 ctgtcggggt ggacgccgtc ttcttcggcg agcgtgacgt tcgggaaagc gcgcggagag
      301 tcttgcttcg gacagctggc gtgctcgctc tcttgtgcgg caccctcctc ggcttgtgta
      361 ttggcgctga agattttctt agaaaggtgt tgtgggtggt ggggctgggc gcgggcgacg
      421 cgtctggcgc ggcggtggct caggtccttc gaatgccgtt gctcacggcg accgcgacgc
      481 tcgtcgtgtg caccgccgcg ttccggagcg catcgcccct cttcaccttc aaagaaaatc
      541 ttcgtcaccc gttgctcctg agcaccctcg tcgcgcttct cttcctcggc gacgcggcgc
      601 gacgcgtcgc cgggacgtgt cccggcgggc cgccggatct ctatcgcaag gagacgcccg
      661 aggtcgccct cgtgcgcgca caagcgggag acggccggtt tttcgacgac ggcgcggacg
      721 accgggccac ggtcgttcgg cgcacgctcg aggcgggagg gctcgacctc ctccggcccg
      781 cgaccggggt ggtcttcgga atccgctacc tcggagacaa cgacgtggac cgcctgacgg
      841 cggctcccgc cgtgcgctgg accgcggcgc tcgcggccat gccgtggggc gaggagaagg
      901 tgcggatcct gcgtgccgcc ggcgtcgcgc tcgtccggac ggcgtcgccc ggaccggatc
      961 ccgccgcgac cgaggagatc ggccggtggg gatcggaccg cttcgtccgg atcgcgggcg
     1021 cgcggccgga gttcgcgctg ctgcccgagg gcgaaggggg ctcggtgctc gtcgtcgagc
     1081 gccggcccgc gcgcgtcgtc ctccgcgccg acgtcacgct tccgtccgcg acgctgtgga
     1141 tcggccggac gttcgacccc aactggcgcg cacacgcgga cggccgggag atcgctctga
     1201 ccccggcgga cggatggcgc atggaagcga agcttccgaa gggcgtccac gacgtcacgc
     1261 tccgctacgc gaacccgctc ttcggcgccg gcctggccgt ctccctggcg gccctcgccg
     1321 ccgcggcggc gctcctccgc gcggggaggc cggcgtgagg ctcaggcgcg cggcgctcct
     1381 cctcggggcg atccttctcg cgtgggtccc gtaccgcgcg ttcttcggga gcagcgttcc
     1441 cgtcggccgc gacctgcttt tctacttcta tccgctcaag gcgcacctag ccgaggggct
     1501 gtcgcgtgga gagcttccgt gggtggaccg cttccgctgg ggaggcgcgc cgctgctcgg
     1561 aggtccgtcg gcggcgccct tcgacccggc gaacctcctc ttcctcgcgc tgcctctcgg
     1621 cgcggcgatg aaggcgtgga tgctcctcca tctcgcgctg ctcgtcgcgg gcttcgccgc
     1681 gttcgcgcgc cggctgggcc tcgcggcggc gccggcggcg ctctcgggcc tcgtcttcgc
     1741 gctcgcgggg acgacggtct cggtcgtgcc gttcccgccg acgctgtcgg cgctgtcggt
     1801 ccttccgtgg ttcgcggcgt tcgtcctcga caccgtgcgc aatccgtcgg cgcgcagcgc
     1861 cgcgaagacc gcggcggcgg ccgcccttgt cgccgtcgcg tcggcgcccg agttcctcct
     1921 ctacggggcc gccgtcgcgg cgatgctc
//
LOCUS       DAOMFY010000467         1947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30893_length_1947_cov_7.670719, whole genome shotgun sequence.
ACCESSION   DAOMFY010000467 DAOMFY010000000
VERSION     DAOMFY010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30893_length_1947_cov_7.670719"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLB01_20055"
     CDS             complement(<1..420)
                     /locus_tag="PLB01_20055"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQQ79647.1"
                     /translation="MKAPVVTGVSAVTALGRTLGETRAALDAGRSAIASIEGPGVPER
                     AERSPAARIGAFTTEPELPKSKARRLDLGSQYALVAARQCLADAGYAVAGREERIGIL
                     FGTGSAGAGPLTGLERQMAQPPEAASPSLFPHTAANAP"
     gene            complement(417..1628)
                     /locus_tag="PLB01_20060"
     CDS             complement(417..1628)
                     /locus_tag="PLB01_20060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HQQ79648.1"
                     /translation="MEGRRVVVTGLGAVTSLGKDLASTWEGLVAGRSGIGPITLFDSS
                     GFRTHLAAEIKELPGGIEPSLRRRLSRTDVAGLGAAAEALADSGLDLARVDPSRIGVV
                     LGAGVSGLLDSENYLHDLLAKGPRAARPTRVLNHPPDQTTDRIAERWGFLGPRSSITT
                     ACSSSATSLGYAADLIRHGYCDVVVTGGADTFARLTHGGFNALRSVDPDPCRPFDLRR
                     KGLTIGEAAGLLVFEEEEHARRRGANLRARFLGYGVTSDAHHMTQPEPTGEAGARTMA
                     ACLKAAGLNPEDVDYVNAHGTATPQNDSAETAAIKRALGARAYEIPVSSIKSMLGHCL
                     CSAGAIEAVATVRTIETGIVPPTINWGERDPECDLDIVPNVAREVRVRAALSNSFAFG
                     GNSTVVAFGAA"
     gene            complement(1639..>1947)
                     /locus_tag="PLB01_20065"
     CDS             complement(1639..>1947)
                     /locus_tag="PLB01_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79649.1"
                     /translation="AARLSETVLSADPANGYARRNLAAALELAGDALSLRPGPPPSAK
                     ALAEARAWYGRALGVWLAMDHERKLHGDDAAAPERIRQALRRCGDPSPGGNPNQASTQ
                     "
BASE COUNT          250 a          667 c          775 g          255 t
ORIGIN      
        1 gggggcgttc gcggccgtgt gcgggaagag ggacggcgac gcggcctccg gcggctgggc
       61 catttgccgc tcgagccccg tcagcgggcc ggcgccggcc gagccggtcc cgaagaggat
      121 gccgatgcgc tcctcgcgac cggcgaccgc gtagcccgcg tccgcgaggc actgccgggc
      181 cgcgaccaag gcgtactggc tgccgaggtc gaggcggcgc gccttgctct tcgggagctc
      241 cggctcggtc gtgaacgcgc cgatgcgggc ggcgggggat cgctccgccc gctccgggac
      301 gcccggaccc tcgatggacg caatggcgga gcgcccggcg tcgagggcgg cacgcgtctc
      361 gccgagcgtg cggccgagcg ccgtgacggc gctgacgccc gtgacgacgg gggccttcat
      421 gcggccccga acgcgacgac ggtggagttg ccgccgaacg cgaacgagtt cgagagggcg
      481 gccctcacgc gcacctcgcg cgcgacgttc gggacgatgt cgaggtcgca ctcgggatcg
      541 cgctcgcccc agttgatcgt cgggggcacg atgcccgtct cgatcgtccg gacggtggcg
      601 accgcctcga tggcgccggc cgagcagagg cagtgcccga gcatggactt gatcgaggac
      661 acggggatct cgtacgcgcg ggctccgagc gccctcttga tggctgcggt ttccgccgag
      721 tcattctgcg gcgttgccgt cccgtgggcg ttcacgtagt cgacgtcttc cgggttcagg
      781 ccggcggcct tcaggcaggc ggccatcgtg cgcgcgcccg cctcgcccgt gggctcgggc
      841 tgcgtcatgt ggtgcgcgtc ggacgtgacg ccgtagccga ggaagcgggc gcgcaggttc
      901 gcgccgcggc gccgggcgtg ctcctcctcc tcgaagacga ggagacccgc ggcctcgccg
      961 atcgtcagcc ccttgcggcg caggtcgaac ggacggcagg ggtcggggtc gacggaccgc
     1021 agcgcgttga acccgccgtg cgtgaggcgc gcgaaggtgt cggcgccgcc cgtcacgacg
     1081 acgtcgcagt aaccgtggcg gatgaggtcg gccgcatagc cgagggacgt cgccgaggac
     1141 gagcaggccg tcgtgatgga cgagcgcggg ccgaggaagc cccaacgctc cgcgatgcgg
     1201 tccgtcgtct ggtcgggcgg gtggttcagg acgcgcgtcg ggcgcgcggc gcgagggccc
     1261 ttcgcgagga ggtcgtgcag gtagttctcg ctgtcgagga ggccggagac tcccgcgccg
     1321 aggacgacgc cgatccgcga ggggtcgacg cgcgcgaggt cgagaccgga atcggcgagc
     1381 gcctcggccg ccgcgccgag gccggcgacg tccgtgcgcg agagccggcg gcggagcgac
     1441 ggctcgattc cgcccggcag ctccttgatc tcggcggcga ggtgggtgcg gaacccggac
     1501 gagtcgaaga gcgtgatcgg accgataccg gagcggcccg cgacgaggcc ctcccacgtg
     1561 gacgcgaggt cctttccgag agaggtcacg gctcccagtc ccgtaacgac gacgcggcgc
     1621 ccttccatcc gccggatctt actgagtcga agcctggttt ggattcccgc ccggcgacgg
     1681 atctccgcag cggcggagcg cctgccggat ccgttccggg gccgccgcgt cgtcgccgtg
     1741 gagcttgcgt tcgtgatcca tcgcgagcca gacgccgagg gcgcgcccgt accacgcccg
     1801 cgcctcggcg agggccttgg cggaaggcgg cggcccgggc cggagcgaga gggcgtcgcc
     1861 agcgagctcc agcgcggcgg cgaggttccg ccgggcatac ccgttcgccg ggtcggcgga
     1921 aaggacggtc tcggagaggc gtgcggc
//
LOCUS       DAOMFY010000468         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30926_length_1946_cov_8.369646, whole genome shotgun sequence.
ACCESSION   DAOMFY010000468 DAOMFY010000000
VERSION     DAOMFY010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30926_length_1946_cov_8.369646"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLB01_20070"
     CDS             <1..779
                     /locus_tag="PLB01_20070"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQQ79650.1"
                     /translation="DGDGRPDLYVTNYANEPNTLYRNVEGATFAESEREAFGGDHDPV
                     LPLSKWGTVALDWDNDGKDDLAVSSGQILSRFYTVLGGWFNPKAKNFAVGEKSYAQRQ
                     FLFHNESAPGTMTFRDVSAEAGDLGRLVIVGRGLSAGDLDGDGKEDLVFNPIDVAAIV
                     LKNTAAGGNALEILPVAGADRRTVLGTRVTAGAKMKEFYVAPSYASGSWLPLHFGLGT
                     AASAHVTVRWPDGSTQDLGDVPKGAWRLKKGGVLEPLRPR"
     gene            complement(780..>1946)
                     /locus_tag="PLB01_20075"
     CDS             complement(780..>1946)
                     /locus_tag="PLB01_20075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ79651.1"
                     /translation="RTEAEAPARIEEIRHAKEEGITFHWLRSPLRIETDEAGDVKAIV
                     TQVMELGPPDKSGRRRPVPVEGETETFLCDTVIYALGTKANPVIARSTPGLETRDEGY
                     INADAKTQAANLPGVFAGGDIVTGGATVILALGAGRRAAKAITQYLKTKEWPVVLADE
                     PAPAPKKAKTTEETDAVKTCPKCRQPLEGEEEYVCCAGLTLTWTCDGCQKVYEGFAFP
                     YGLCPACGGTLSHGHDVKLDNERAVEALRHAFEIELGGLAFYAHGAETTQDPALKALF
                     TSLSEMERGHMETLSRRYHVPEPDTAAPGLSAARVAVYAEAGQKTETGEDLLKLAVHL
                     ERRARNFFLERGRELEPGSDEWKLYREMEAEEREHTDLLTTALVRYTAGKAVLV"
BASE COUNT          264 a          665 c          712 g          305 t
ORIGIN      
        1 tcgacggcga cggccgcccc gacctctacg tcacgaacta cgcgaacgag ccgaacacgc
       61 tctaccgcaa cgtcgagggc gcgacgttcg ccgagagcga gcgcgaggcg ttcggcggcg
      121 accacgaccc cgtcctgccg ctttcgaagt gggggaccgt ggcgctcgac tgggacaacg
      181 acgggaagga cgacctcgcc gtctcgagcg gccagatcct ctcgcgcttc tacacggttc
      241 tcggcggctg gttcaacccc aaggccaaga acttcgcggt gggagagaag agctacgccc
      301 agcggcagtt cctcttccac aacgagagcg cgcccgggac gatgacgttc cgggacgtct
      361 ccgcggaggc gggtgacctc gggcgactcg tgatcgtggg gcgcggactc tcggcggggg
      421 acctcgacgg ggacgggaag gaagacctcg tcttcaaccc gatcgacgtg gccgcgatcg
      481 tcctgaagaa cacggcggcg ggagggaacg ccctggagat ccttccggtc gcgggcgcgg
      541 accggaggac cgtcctcgga acgcgcgtca cggcgggggc gaagatgaag gagttctacg
      601 tcgccccgtc gtacgcgagc ggctcgtggc tgccgctgca cttcggcctc gggacggccg
      661 cgtccgcgca cgtgacggtc aggtggccgg acggctcgac gcaggacctc ggcgacgtcc
      721 cgaagggggc gtggaggctg aagaagggcg gagtgctgga gcccctccgc cccaggtgat
      781 cagacgagga cggccttgcc cgccgtgtac cgcacgagcg cggtcgtgag caggtccgtg
      841 tgctcgcgct cctcggcctc catctcgcgg tagagcttcc actcgtccga gccgggctcg
      901 agctcgcggc ccctctcgag gaagaagttc cgggcgcgcc tctcgaggtg gacggcgagc
      961 ttcaggaggt cctcgccggt ctcggtcttc tggccggcct cggcgtagac ggcgacgcgc
     1021 gcggccgaga ggcccggcgc ggccgtgtcc ggctcaggca cgtggtagcg gcgcgagagc
     1081 gtctccatgt gcccgcgctc catttcggag aggctcgtga agagcgcctt gagggcgggg
     1141 tcctgcgtcg tctccgcgcc gtgcgcatag aacgcgaggc cgccgagctc gatctcgaac
     1201 gcgtgccgaa gggcctcgac ggcccgctcg ttgtcgagct tcacgtcgtg cccgtgcgag
     1261 agcgtgccgc cgcaggccgg gcagaggccg tacgggaacg cgaagccctc gtagaccttc
     1321 tggcatccgt cgcacgtcca cgtgagcgtg aggcccgcgc agcagacgta ctcctcctcg
     1381 ccctcgaggg gctggcggca cttcgggcag gtcttcacgg cgtccgtctc ctcggtcgtc
     1441 ttcgccttct tcggggctgg cgcgggctcg tcggcgagga cgaccggcca ctccttcgtc
     1501 ttcaggtact gcgtgatcgc cttggccgcg cggcgcccgg cgccgagcgc gaggatgacg
     1561 gtcgcgccgc cggtcacgat gtcgccgccc gcgaagacgc cgggcaggtt cgcggcctgt
     1621 gtcttcgcgt ctgcgttgat gtagccctcg tcccgcgttt cgaggcccgg ggtcgagcgc
     1681 gcgatgacgg ggttcgcctt cgtcccgagc gcgtagatga ccgtgtcgca gaggaacgtc
     1741 tcggtctcgc cctcgacggg gacggggcgg cgccggccgg acttgtccgg cgggccgagc
     1801 tccatcacct gcgtgacgat ggccttcacg tcgccggcct cgtccgtctc gatgcggagc
     1861 gggctcctga gccagtggaa ggtgatcccc tcctccttgg cgtggcggat ctcctcgatg
     1921 cgcgcgggag cctcggcctc ggtgcg
//
LOCUS       DAOMFY010000469         1944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_30970_length_1944_cov_6.755956, whole genome shotgun sequence.
ACCESSION   DAOMFY010000469 DAOMFY010000000
VERSION     DAOMFY010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30970_length_1944_cov_6.755956"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1350
                     /gene="asd"
                     /locus_tag="PLB01_20080"
     CDS             274..1350
                     /gene="asd"
                     /locus_tag="PLB01_20080"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQQ79652.1"
                     /translation="MSRPDSRIPVAVLGATGAVGQRLVSLLEGHPQFVLAEVAASERS
                     AGKTYAEATRWILPGDVPQEARGLVVTQVDADFVSPVLLSGLDAAVADTIEPLQAGRG
                     KLVVSNTKSFRMRPDVPLVIPEVNADHLALLDGQPTRASGGGIVTNPNCSVVGLAMAL
                     APLHRAFGVEAVAVTTLQALSGAGYPGVPSLDANANVIPFIDGEEEKIEREPKKILGR
                     LEGGRVVDADFPISVSVNRVPVRDGHTESVFLKLRAKASLADVRAALEEFSGEPQRLK
                     LPSAPAQPIAVRDERDRPQPIRDVEAGNGMTVTVGRLRPDPVYDARFTLLVHNTVRGA
                     AGAALLNAELLLAQGRLAARTAAR"
     gene            1355..>1944
                     /locus_tag="PLB01_20085"
     CDS             1355..>1944
                     /locus_tag="PLB01_20085"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79653.1"
                     /translation="MTDVLKFGGTSVGTAERIRDVARLVAGRGGDPCVVVVSAMGGIT
                     DLLVSLKSSSSERDRAQAERAREALAQRHVAALAGLGLPAAELTAMSDALASELRRAE
                     ELSAGIFLLEEVSLRTADALLCLGEMISSRLVAAAARAAGADAVWVDPREILAYEGLE
                     IRRERLDASAHAGARHAAVLAEHRDDVGATHTQHRAL"
BASE COUNT          281 a          699 c          673 g          291 t
ORIGIN      
        1 ccaaggggac atttctatcg agttatcgag gggacacttt catggagcta cgacaaggtg
       61 cggctgaact cttgacaccc gtctgccgcc cgcctacgct ccgcgcgcat gaacgccgcc
      121 gccatctccc gaaagcggat gaccgcgaag aagacgacgc cgccgtgcgg cgggcgatgg
      181 agcgttctgt aggtcgcggt tcaagaggcg atcaccagat gacaccaggg cccgccggaa
      241 acggcgggcc ttttcgtttc gaaggagtcc accatgtccc gtcccgattc ccgcattccc
      301 gtggccgtcc tcggcgcgac cggcgccgtc gggcagcgcc tcgtctcgct gctcgaggga
      361 cacccgcagt tcgttctcgc ggaagtcgcc gcctccgaac ggtccgccgg gaagacgtac
      421 gccgaggcga cgcgctggat cctgccgggc gacgtcccgc aggaggcccg cggcctcgtc
      481 gtgacgcagg tcgacgcgga cttcgtctct ccggtgctcc tctcgggcct cgacgcggcc
      541 gtcgccgaca cgatcgagcc gctgcaggcc ggccgcggca agctcgtcgt gtccaacacg
      601 aagtccttcc ggatgcgtcc cgacgtgccg ctcgtgatcc ccgaggtcaa cgcggaccat
      661 ctcgccctcc tcgacggcca gccgactcgc gcgtccggcg gcggcatcgt gacgaatccg
      721 aactgctccg tcgtggggct cgcgatggcg ctcgcccctc tccaccgcgc gttcggtgtc
      781 gaggccgtcg ccgtcacgac gctgcaggcg ctctccggcg cgggctatcc cggggtcccg
      841 tcgctcgacg cgaacgcgaa cgtaatcccg ttcatcgacg gcgaggagga gaagatcgag
      901 cgcgagccga agaagatcct cggccggctc gagggcgggc gcgtcgtgga tgcggacttc
      961 ccgatcagcg tctccgtgaa ccgggtgccc gtgcgcgacg gccacacgga gtcggtcttc
     1021 ctcaagctca gggccaaggc gtctctcgcg gacgtgaggg ccgccctcga ggaattctcg
     1081 ggcgagcctc agcggctgaa gcttccgtcg gcgccggcgc agcccatcgc cgtccgggac
     1141 gaacgggacc ggccgcagcc catccgcgac gtggaggccg ggaacggcat gaccgtcacc
     1201 gtcggccgcc tcaggcccga tcccgtctac gatgcgcgct tcactctcct cgtccacaac
     1261 actgtgcgcg gcgcggcggg cgcggcgctc ctgaacgccg agctcctcct cgcgcagggc
     1321 cggctggcgg cccgaacggc cgcgcggtga acgaatgacc gacgtcctca agttcggcgg
     1381 gacctcggtc ggaacggccg agcggatccg ggacgtcgcg cgcctcgtgg cggggcgcgg
     1441 gggagacccg tgcgtcgtcg tggtctcggc catggggggg atcaccgacc ttctggtgag
     1501 cctgaagagt tcttcgagtg agagagatcg ggcgcaggcc gagcgcgcga gggaggctct
     1561 cgcgcagcgt cacgtcgcgg cgcttgcggg gctgggtctg cccgcggcgg agctcacggc
     1621 aatgagcgac gcgctggcct ccgagctccg gcgggccgaa gagctctccg ccggcatctt
     1681 cctactcgaa gaagtctctc ttcggacggc agatgcgctt ctctgcctgg gagagatgat
     1741 ctcctcccga ctcgtcgccg ccgcggcgcg cgcggcgggc gccgacgcgg tctgggtgga
     1801 cccgcgcgag atcctcgcgt acgaaggtct cgagatccgg cgcgagcggc tcgacgcctc
     1861 cgcgcacgct ggcgcacgcc acgctgccgt ccttgcggag caccgcgacg acgtcggagc
     1921 cacgcacacc caacatcgtg ccct
//
LOCUS       DAOMFY010000470         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_31801_length_1917_cov_9.006982, whole genome shotgun sequence.
ACCESSION   DAOMFY010000470 DAOMFY010000000
VERSION     DAOMFY010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31801_length_1917_cov_9.006982"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLB01_20090"
     CDS             <1..506
                     /locus_tag="PLB01_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79654.1"
                     /translation="AGRARRAEALGTSERVGNIVRSLAEGAPSPPPGSALLLLDADGP
                     PRAEDVLGLTLTDVHLADALRVVHRQADLLVYVYGRDLRYRWNGTPEARADGLEVRWG
                     DASSALVPWEKVAVFHEDPSGGVTRLDELPRGAFPFTAPPDLGRLVPRTGPTPPRLAT
                     LCPQRTR"
     gene            complement(491..1795)
                     /locus_tag="PLB01_20095"
     CDS             complement(491..1795)
                     /locus_tag="PLB01_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQQ79655.1"
                     /translation="MLSLAAPAAVVERLPPMREQHAIRQEWVKERLEKVVPALMRKHG
                     VAMWLVVCREYHEDPVFFSLVSPSTMAARRRTILVFFDRGGDKGVERLALGGGSNGGL
                     YTVYRDPEVENRELWGNAQWALLRKLVDEKKPANIAVDISRTHAFSDGLSAGDREELE
                     EALGPWKEKIVRAELLPLEVIETRVPGMLPTYRRMMAIAHGIIAKAFSAEVITPGVTT
                     TEDVVWWMRQRVNDMGLGEWFPPTVDVQRPGVTAKSSLAETKPVVIRRGDVLHCDFGI
                     RAMGLNTDTQHMAYVLKEGETDAPEGLRKALANSNRLQDLVLARMKPGRTGNEVLLDA
                     LAAAKAEGLEGSVYSHPIGDHGHGAGPIVGLWDRQTAIPGRGDVKIVPSTWFSIELQA
                     TTPVPEWGGQPVSMMQEEDAAIADDGTIAWVLPRQTKFHLVR"
BASE COUNT          254 a          704 c          667 g          292 t
ORIGIN      
        1 ccgccgggcg ggcccgccgc gccgaggccc tcgggacgtc ggagcgcgtc ggcaacatcg
       61 tccggtcgct cgccgagggc gcgccctcgc cgccaccggg ttcggctctt ctcctgctcg
      121 acgcggatgg tccgccgcgc gcggaggacg tcctcgggct gacactcacg gacgtccacc
      181 tcgccgacgc cctccgggtc gtccaccggc aggcggatct cctcgtctac gtctacggcc
      241 gcgacctgcg ttaccgctgg aacgggacgc cggaggcccg agcggacggc ctggaggtgc
      301 gctggggcga cgcgtcctcg gcgctcgtcc cgtgggagaa ggtcgccgtg ttccacgagg
      361 acccgagcgg aggcgtgacg cggctggacg agctcccgcg cggcgcgttt ccgttcacgg
      421 ccccgccgga cctcgggcgg ctcgtcccgc ggacggggcc gacgccgccg cgccttgcga
      481 cgctctgccc tcagcgcacg aggtgaaact tcgtctggcg cgggaggacc cacgcgatcg
      541 tcccgtcgtc ggcgatcgcc gcgtcctcct cttgcatcat cgagacgggc tggccgcccc
      601 actcgggcac gggcgtcgtc gcctgcagct cgatcgagaa ccacgtcgag gggacgatct
      661 tcacgtcgcc gcgcccggga atcgccgtct ggcggtccca caggcccacg atcgggcccg
      721 cgccgtggcc gtggtcgccg atcgggtgcg agtagaccga accctcgagg ccttcggctt
      781 tcgcggccgc gagcgcgtcg aggagcacct cgttgccggt ccggcccggc ttcatccgcg
      841 cgaggacgag gtcctgcagg cggttcgagt tcgcgagcgc cttcctcagg ccctcgggcg
      901 cgtccgtctc gccctccttg aggacgtacg ccatgtgctg cgtgtcggtg ttgagcccca
      961 tcgcccggat gccgaagtcg cagtgcagga cgtcgccccg ccggatcacg acgggcttcg
     1021 tctcggcgag gctgctcttg gccgtgacgc ccggccgctg gacgtcgacc gtcggcggga
     1081 accactcgcc cagccccatg tcgttcacgc gctggcgcat ccaccagacg acgtcctcgg
     1141 tcgtcgtcac gcccggcgtg atgacctcgg ccgagaacgc cttcgcgatg atcccgtgcg
     1201 cgatcgccat catccgccgg tacgtcggga gcatgccggg cactcgcgtc tcgatgacct
     1261 cgagagggag gagctcggcg cggacgatct tctccttcca cgggccgagc gcctcctcca
     1321 gctcctcgcg gtcgcccgcg gagaggccgt cggagaacgc gtgcgtcctc gaaatatcca
     1381 cggcgatgtt cgcgggcttc ttctcgtcca cgagcttgcg gagcagcgcc cactgggcgt
     1441 tgccccagag ctcgcggttc tcgacctcgg ggtcgcggta caccgtgtag aggccgccgt
     1501 tcgagccgcc gccaagggcg aggcgctcga cgcccttgtc gccgccgcgg tcgaagaaga
     1561 cgaggatcgt ccggcggcgc gcggccatcg tcgacggcga gacgagcgag aagaagaccg
     1621 ggtcttcgtg gtactcgcgg cacacgacga gccacatcgc gacgccgtgc tttctcatga
     1681 gcgccggcac gaccttctcg agccgctcct tcacccactc ctgccgaatc gcgtgctgct
     1741 cgcgcatggg cggcagcctc tcgacgacgg cggccggcgc cgcgagcgaa agcaagagaa
     1801 gaaaggagag atttctgcga atgaaagaga ttgggggcac gccgtcacct cgccgtttcg
     1861 cggacgcgag tgtaacggcg tcggggcccc ttcaggcgcg cgagcggccc tcggccg
//
LOCUS       DAOMFY010000471         1915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_31878_length_1915_cov_3.352151, whole genome shotgun sequence.
ACCESSION   DAOMFY010000471 DAOMFY010000000
VERSION     DAOMFY010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31878_length_1915_cov_3.352151"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLB01_20100"
     CDS             complement(<1..791)
                     /locus_tag="PLB01_20100"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HQQ79656.1"
                     /translation="MIKERQSGAQPPGPSKKIFSSSSSSVSVFAPASVANFGVGFDVL
                     GAALEGPGDVVRARLTEKPGVRLVGISGDGGRLPLSARRNTAAVAAAAVLEAAGERRR
                     GVELTLEKGLPLSSGMGSSAASAAAGALAAGLLLGVTDRFALLRPALLGEHVADGSWH
                     GDNVFASLLGGFVLVPSSDPDGPLAPVRLEAPARLRLVLVHPALELETRRARGVVPRK
                     IPMAVHTAHAASLASLIAALADGDLARAGRTLLEDRLVDPARLPL"
     gene            complement(788..>1915)
                     /locus_tag="PLB01_20105"
     CDS             complement(788..>1915)
                     /locus_tag="PLB01_20105"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQQ79657.1"
                     /translation="LGVRFDASPLRELSAALEGVALLRERTARVTDLVSSFGERLSAP
                     LLAATLVKAGVPARAVDAREVLVTDDRHGAATCDRRKSNPRVKRRLRPLLSGGILPVV
                     TGFIARNAGGETTTLGRSGSDTTAALLGAALGAKEVVIWTDVDGVLSADPRLVPGARL
                     LRRLSYREAAEMTYFGAKVLHFPAVLPAIAEEIPLVIRNSFRPERPGTTIAVKGDGAR
                     GVRAVTAISGLCLLSLDGKGMTGIPGIAARVFGATARLGVSVVMFSQASSEQNIALVI
                     PEGDRARTVAALDQEFRFERLTGSIDGVTARTPIAVVAAVGDGMRGTPGIAAKVFAAA
                     ARAGVNVEAIAQGSSECNISFAVAGEDREKAVRALHRVVTT"
BASE COUNT          282 a          689 c          732 g          212 t
ORIGIN      
        1 atgagcggca gacgggcggg gtcgacgagc cggtcctcga gcagggtgcg acccgcgcgc
       61 gcgaggtcgc cgtccgcgag ggcggcgatc aggctcgcga gtgacgccgc gtgggcggtg
      121 tgcaccgcca ttgggatctt tcgcgggacg acgccgcgcg cgcggcgcgt ctcgagctcg
      181 agcgccggat ggacgaggac gaggcgcagg cgcgcgggcg cctcgaggcg caccggcgcg
      241 agcggcccgt ccgggtcgga ggacgggacg aggacgaacc cgccgagcag cgaggcgaag
      301 acgttgtctc cgtgccacga gccgtccgcg acgtgctcgc cgaggagcgc cgggcgcagg
      361 agcgcgaagc gatccgtcac gcccaggagc agtccggcgg cgagcgcgcc cgcggcggcc
      421 gaggctgccg acgagcccat gccgctcgag agcgggagac ccttctcgag cgtcagctcg
      481 acgccgcgcc gccgctcccc cgccgcttcg agcacggcgg cggcggcgac cgcggccgtg
      541 ttccggcgcg ccgagagcgg caggcgtccg ccgtcgcccg agatcccgac gagccggacg
      601 cccggcttct ccgtcaggcg cgcgcggacg acgtcgcccg gaccctcgag ggcggcgccg
      661 aggacgtcga acccgacgcc gaagttcgcg accgacgccg gggcgaagac cgagaccgaa
      721 gaggaagacg aagagaagat tttcttagaa ggtccaggag gctgcgcgcc ggactgcctc
      781 tccttgatca tgtcgtcacg actcgatgca gcgccctaac ggccttctcg cggtcctcac
      841 cggccacggc aaaactaatg ttgcattccg agctgccttg ggcgatggct tccacgttga
      901 cgccggcccg cgcagcggcc gcgaagacct tcgccgcgat cccgggcgtc ccgcgcatgc
      961 cgtcgcccac ggccgcgacg accgcgatcg gcgtgcgcgc cgtgacgccg tcgatcgagc
     1021 ccgtgaggcg ctcgaagcgg aactcctggt cgagcgcggc gaccgtgcgc gcgcggtcgc
     1081 cctcgggaat gacgagcgcg atgttctgct ccgaggaggc ctgcgagaac atgacgaccg
     1141 agacgccgag gcgcgccgtc gcgccgaaga cgcgcgccgc gatcccgggg atcccggtca
     1201 tgcccttgcc gtcgagggag aggaggcaca acccggagat cgccgtgacg gcgcggacgc
     1261 cgcgcgcgcc gtctcccttg accgcgatcg tcgtccccgg gcgctccggg cggaacgagt
     1321 tgcggatgac gagcgggatc tcctcggcga tcgccgggag gacggccggg aagtggagga
     1381 ccttcgcgcc gaagtacgtc atctccgccg cctcgcggta ggagaggcgc cggaggaggc
     1441 gcgcgccggg cacgaggcgc gggtccgccg acaggacgcc gtccacgtcc gtccagatca
     1501 cgacctcctt cgcgccgagg gcggcgccga ggagggcggc cgtcgtgtcg gagcccgagc
     1561 ggccgagagt cgtcgtctcc cctcccgcgt tcctcgcgat gaagcccgtc acgaccggga
     1621 ggatgccgcc cgaaaggagc ggccggagcc gccgcttcac acgcgggttg ctcttcctgc
     1681 ggtcgcacgt cgcggcgccg tgccggtcgt ccgtcacgag cacctcgcgc gcgtccacgg
     1741 cccgcgccgg gacgcccgcc ttcacgagcg tggcggcgag gagcggcgcc gagagacgct
     1801 cgccgaagga ggagacgaga tcggtgacgc gcgccgtccg ctcgcgcagg agcgcgacgc
     1861 cctcgagggc ggcggagagc tcccggagcg gcgacgcgtc gaaacgcacg ccgag
//
LOCUS       DAOMFY010000472         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_31897_length_1914_cov_7.622378, whole genome shotgun sequence.
ACCESSION   DAOMFY010000472 DAOMFY010000000
VERSION     DAOMFY010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31897_length_1914_cov_7.622378"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLB01_20110"
     CDS             complement(<1..630)
                     /locus_tag="PLB01_20110"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQQ79658.1"
                     /translation="MQVRRLSDGTAVWLDPMRDVKSLALGIYVAAGSADEAPRERGAT
                     HFLEHLLFRRTRRRSGAAIARITDRLGGDCDAYTTKECVAFHARVPAARAADAVDLLL
                     DLTSAPAFTSEDVEVERGVILEEIAEAHDVPDDLLHDTFVRALWPAHDLGEPIFGSKE
                     SVSALTRAALAARFRAIFRPERTLVVAAGAMDPDRLVALLERGRLRRAPM"
     gene            703..>1914
                     /locus_tag="PLB01_20115"
     CDS             703..>1914
                     /locus_tag="PLB01_20115"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQQ79659.1"
                     /translation="MGLGRRVFANALLLAVSLAAVLAAAEAGLRAFFPQPLLETTRAA
                     DTGGPLTRRDAELGWALKSDVAWPVGSAPWENGLTTNAAGFRDAPHAEVKAPGAYRIA
                     VLGDSFVFGSGVAQDETLTRRLAAHLGPGFEIVNLGVPGYGTDQELLTLRRWGRKLSP
                     DLVLLAFFWNDLMENVSDRIYGLPKPRFTLEGGRLVPHPPAGFSAPSTFARLDASLGS
                     RSHLWSLLRSGLSSAGRAMGSAREERSVSIDFSLKSPPASREAEFALAFALLGAVRDE
                     AASLGAPLGVFSVPPRFLVEPAVAARLLKVYGLPGDALEEDGFRRLREACEARGIPFV
                     DLVPAFRRETAEGARLFLPTGIHWSAAGNDAAARHLAPAVRAGFKPVSARSPVPGVPG
                     VSETVRVRSIVS"
BASE COUNT          237 a          727 c          684 g          266 t
ORIGIN      
        1 catgggggcg cgccgcaggc ggccccgctc gaggagcgcc acgagacggt ccggatccat
       61 cgcgccggcc gcgacgacga gcgtccgctc gggccggaag atcgcgcgga accgcgccgc
      121 gagcgcagcg cgcgtcaggg cggacacgct ctccttcgac ccgaagatgg gctcgccgag
      181 gtcgtgggcc ggccagagcg ccctcacgaa cgtgtcatgg aggaggtcgt ccggcacgtc
      241 gtgcgcctcg gcgatctcct cgagaatgac gccgcgttcg acctccacgt cctcggacgt
      301 gaacgcagga gccgacgtca ggtcgagaag gaggtcgacg gcatcggccg cgcgcgccgc
      361 cgggacgcgc gcgtggaacg cgacgcactc tttcgtggtg tacgcgtcgc agtcgccgcc
      421 gaggcggtcc gtgatccgcg cgatcgccgc gccggagcgc ctgcgggtcc tcctgaagag
      481 gaggtgctcg agaaagtgcg tcgcgccgcg ctcgcggggc gcctcgtccg ccgagcccgc
      541 cgcgacgtag atcccgaggg ccaggctctt cacgtcgcgc atgggatcca gccacaccgc
      601 cgtcccgtcc gagagccgcc gaacctgcac gcccgattct agtcggcgaa gcttgtcccg
      661 gaacggccgc cgcaccacaa tcgagggcgg gggggtcgga acttgggtct cggccggcgt
      721 gtcttcgcga acgcgcttct cctcgccgtc tccctcgccg cggttctcgc ggcggcggag
      781 gccgggctga gggcgttctt tccgcagccg cttctcgaga ccacccgcgc cgcggacacg
      841 gggggccccc tcacgcggcg cgacgccgag ctcggatggg cgctcaagtc cgacgtcgcg
      901 tggccggtcg gctccgcccc gtgggagaac ggtctcacga cgaacgcggc gggcttccgc
      961 gacgcccctc acgcggaggt gaaggcgccg ggcgcgtacc ggatcgcggt gctcggcgac
     1021 tcgttcgtgt tcggaagcgg cgtcgcacag gacgagaccc tgacgcggcg gctcgcggcg
     1081 cacctcgggc ccggcttcga gatcgtgaat ctcggcgtcc cgggctacgg gacggaccag
     1141 gagctgctca ccctccgccg ctggggccgg aagctctccc ccgacctcgt cctgctcgcg
     1201 ttcttctgga acgacctcat ggagaacgtg agcgaccgga tctacggcct gccgaagccg
     1261 cgcttcacgc tcgagggcgg acgcctcgtc ccgcacccgc ccgccgggtt ctcggcgccg
     1321 tcgacgttcg cccgcctcga cgcgtccctc gggagccggt cccacctgtg gtcgctcctg
     1381 agaagcggac tctcgtcggc cggccgggcc atgggcagcg cacgcgagga acgctccgtc
     1441 tcgatcgact tctcgctgaa gagcccgccc gcgtcccgcg aggcggagtt cgcgctcgcg
     1501 ttcgcgctcc tcggcgccgt ccgcgacgag gccgcgtcgc tcggcgcgcc gctcggcgtc
     1561 ttttccgtgc cgccgcggtt cctcgtcgag ccggcggtcg ccgcccgcct cctgaaggtc
     1621 tacggcctgc ccggcgacgc gctggaagaa gacggcttcc gtcgcctgcg cgaggcgtgc
     1681 gaggcgcgcg gaatcccgtt cgtcgacctc gtgccggcct tccggcgcga gaccgccgaa
     1741 ggcgcaaggc tcttcctgcc gaccggcatc cactggagcg ccgccggcaa cgacgccgcg
     1801 gcacggcacc tcgcccccgc ggtgcgcgcc ggcttcaagc ccgtcagcgc ccgatccccg
     1861 gtcccggggg tcccgggggt ctcggagacg gtccgcgtcc ggtcgatcgt cagc
//
LOCUS       DAOMFY010000473         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_32428_length_1898_cov_6.585458, whole genome shotgun sequence.
ACCESSION   DAOMFY010000473 DAOMFY010000000
VERSION     DAOMFY010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32428_length_1898_cov_6.585458"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLB01_20120"
     CDS             complement(<1..561)
                     /locus_tag="PLB01_20120"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQQ79660.1"
                     /translation="MLRSIRPSAALLAGLVAASMPGRPAAAAPPALQTAVVAPLSGIV
                     RADGRPLPGATLIVRGVTAGGATLTRTLKSDPDGTFVLSDAAEGLYTVLSVVPGFRPA
                     IARLFHRPAGPEGALSFVRIDLERPSGVLPEAPAGVLDPWSARAVVAGDVLREVPAIF
                     AALDAAPAPAPSTPLLASARDARAAIP"
     gene            complement(629..1537)
                     /locus_tag="PLB01_20125"
     CDS             complement(629..1537)
                     /locus_tag="PLB01_20125"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HQQ79661.1"
                     /translation="MTAFDRLVVSTLPLVPKAIVKQVASRYVAGDTLDAALDTMRTLA
                     AEGAMGTMDVLGESVTHRDQTIATRDQYLRTIDAIAASGLPANISVKPTAVGLAIDPA
                     LALENCMAICTRAAQHGMFVRLDMEDSPYTEATVKLVLALKDRFEVGAVLQAYLKRSL
                     ADLGRLLEKKVNVRICKGIYVEPRAIAWKDRQTIIRNFAALVDKQLAGGAYAAIATHD
                     EACVQLALATIDRLGIPPDRYEFQMLLGVDPGLRRSLIEAGHRLRVYVPYGKDWYAYS
                     TRRLKENPSIARHVLRSMAGMGPAAG"
     gene            complement(1534..>1898)
                     /locus_tag="PLB01_20130"
     CDS             complement(1534..>1898)
                     /locus_tag="PLB01_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQQ79662.1"
                     /translation="GRAPAAHGPRTRRRNRARGYDPAALLAESAARRLRLPARALLSR
                     TRDTHPQSDLPAGARRANVEQAFAAKESARGSVLVLVDDVSTTGATLFSAASALALAG
                     AAEIRGLVLARTPEPEPS"
BASE COUNT          267 a          629 c          752 g          250 t
ORIGIN      
        1 ggggatggcg gcgcgcgcgt cgcgggcgga ggcgagaagc ggagtggagg gcgcgggcgc
       61 aggcgcagcg tcgagcgccg cgaagatcgc gggcacctcg cgcaggacgt cgccggcgac
      121 taccgcgcgc gcgctccacg ggtcgaggac gcccgccggg gcctccggca gcacgccgga
      181 cggcctctcg aggtcgatgc ggacgaacga gagcgcgcct tccggacccg ccggacggtg
      241 gaagaggcgg gcgatcgcgg gccggaagcc gggcaccacg gacaggacgg tgtagaggcc
      301 ctcggcggcg tccgagagta cgaacgtccc gtcgggatcg ctcttcaggg tgcgcgtgag
      361 cgtcgcgcct cccgccgtca cgcctcgcac gatgagcgtt gcacccggga gggggcggcc
      421 gtctgcgcgg acgatgccgg acagcggcgc gaccaccgca gtctggagcg cggggggcgc
      481 ggcggcggcc ggacgtcccg gcatcgacgc ggccacaagg ccggcgagca gggcggcgga
      541 cgggcggatc gatcgcagca cgggaacccc cggtccgcgt atcttccgcc cgttttccgc
      601 acggcgtcaa cgccgttgag agaatctctc agccggcggc cggacccatc cccgccatgg
      661 acctcaggac gtgccgggcg atcgaggggt tctccttcag ccggcgggtg gaataggcgt
      721 accagtcctt cccgtacggg acgtagaccc gcagccggtg gccggcctcg atgaggctcc
      781 tgcggaggcc cgggtccacg ccgaggagca tctggaactc gtagcggtcg ggggggatcc
      841 cgagccggtc gatcgtcgcg agggcgagct ggacgcaggc ttcgtcgtgc gtggcgatcg
      901 ccgcgtaggc gcctccggcg agctgcttgt ccacgagagc ggcgaagttc cggatgatcg
      961 tctggcggtc cttccaggcg atcgcccgcg gctcgacgta gatgcccttg cagatccgta
     1021 cgttgacctt cttctcgaga agcctgccga ggtctgcgag gctgcgcttg aggtaggcct
     1081 gcaggacggc gccgacctcg aagcggtcct tgagcgcgag gacgagcttc acggtcgcct
     1141 ccgtgtacgg gctgtcctcc atgtcgaggc gcacgaacat cccatgctgc gcggcccgcg
     1201 tgcagatcgc catgcagttc tcgagggcga gcgcggggtc gatcgcgagg ccgacggcgg
     1261 tgggcttcac ggagatgttc gccgggagac ccgacgcggc gatcgcgtcg atcgtgcgaa
     1321 ggtactggtc gcgcgtcgcg atcgtctggt cccggtgcgt gacgctctcc ccgaggacat
     1381 ccatcgtgcc catcgcaccc tcggcggcga gcgtgcgcat cgtgtcgaga gcggcgtcga
     1441 gcgtatcgcc ggcgacgtag cgcgaggcca cctgcttgac gatggccttc gggacgaggg
     1501 gaagcgtcga gacgacgagg cggtcgaatg cggtcatgag ggctccggct ccggcgtgcg
     1561 ggcgaggacg agcccgcgga tttcggcggc gcccgcgagg gcgagggcgc tcgcggcgga
     1621 aaacagggtg gcgcccgttg tcgagacgtc atccacgagg acgaggacgc tccctcgcgc
     1681 cgactccttc gcggcgaacg cctgctcgac gttcgcccgg cgcgcgcccg ccggaagatc
     1741 ggactgggga tgcgtgtcgc gcgtgcgcga gagcagcgcc cgggcgggaa ggcgaaggcg
     1801 gcgggcggcg gactccgcga gaagcgccgc gggatcgtag ccccgcgcgc ggttgcggcg
     1861 gcgggtccgc ggtccgtgcg ccgcaggagc gcggcctc
//
LOCUS       DAOMFY010000474         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_32497_length_1896_cov_5.909288, whole genome shotgun sequence.
ACCESSION   DAOMFY010000474 DAOMFY010000000
VERSION     DAOMFY010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32497_length_1896_cov_5.909288"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1896)
                     /locus_tag="PLB01_20135"
     CDS             complement(<1..>1896)
                     /locus_tag="PLB01_20135"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ79663.1"
                     /translation="VLFGRALSPRVAVSDGPGSAPPAPSVTLDGAPWTGTPISAEGTH
                     LLAATVTDCAGHSVSAHALFTIDVTPPRLLTTSPADGAALGAAVASFSGTSDPDLAAA
                     TVNGAAATVSGGTFVATPFSSKEGANTASIVLVDRAGNRGTFSRSFNVKTLAPSVEIL
                     ESGAPLGVGARFFRSVAPVVRSNDPTATVLATLNGVSFLSGTSISASGDYTLNATATD
                     ALGHSSSASASFKVDLSVGPAVDITAPADRAVLPGPTVAVTGTAGASVTSVTVNGRAA
                     TLTGATWSLPDLVLPPDVPSEIVAIAADAAGRTASATRQVTVRASGPQITILEPTSGA
                     RTNRKKIDVAGAVVGGPSATANGTVTVAGQTLALDALGTFRARDVSLQAGANTLTASA
                     TDALGRTGTASVTVTTDTAAPGISLAADGQPLAEGASFARPFTLRVTVTDDSTPAPIP
                     TIRLNGADKAATAAVTDLAIAQAGGYVLSVVARDAAGNESRADRSFVLASGGCAVSAL
                     DPGDGAIVAAPKVTLKGRSGDAASVTVTSGGQTFATQLADGTFAAGDVPLAAVGVNTL
                     AIACTDRAGAVSTTNLTITRLQDGAGPVVRIVQPAGGARQAGATVAVTGTVSDPAAAV
                     TVNGVKAA"
BASE COUNT          251 a          668 c          745 g          232 t
ORIGIN      
        1 gccgccttga cgccgttcac ggtgaccgcg gccgccggat ccgagaccgt gcccgtcacg
       61 gcgaccgtcg cgcccgcctg gcgcgcgccg ccggcgggct ggacgatgcg cacgacgggg
      121 cccgcgccgt cctggaggcg cgtgatcgtc aggttcgtcg tcgaaacggc gcccgcgcga
      181 tccgtgcacg cgatcgcgag cgtgttcacg cccacggccg cgagcggcac gtcgcccgcc
      241 gcgaacgtgc cgtccgcgag ctgcgtcgcg aacgtctggc cgccggacgt caccgtgacg
      301 gacgccgcgt cgccgctccg ccctttgagc gtcaccttcg gggccgcgac gatcgcgccg
      361 tcgcccgggt cgagcgccga caccgcgcac ccgcccgacg cgagaacgaa gctgcggtcg
      421 gcgcggctct cgttgcccgc ggcgtcgcgc gcgacgaccg agaggacgta gccgcccgcc
      481 tgcgcgatcg cgaggtccgt cacggccgcg gtggcggcct tgtcggcgcc gttcagccgg
      541 atcgtgggga tcggcgccgg cgtcgaatcg tcggtgacgg tcacccgcag cgtgaacggc
      601 cgcgcgaagc tcgcgccctc ggcgagcggc tggccgtcgg cggcgaggga gatgccgggc
      661 gccgccgtgt cggtcgtgac cgtgaccgac gccgtgcccg tccggccgag agcgtccgta
      721 gccgaggccg tgagcgtgtt cgcgccggcc tgaagagaaa cgtccctggc gcggaacgtc
      781 ccgagcgcgt cgagcgcgag cgtctggccc gcgacggtga ccgtgccgtt cgccgtggcg
      841 gacgggccgc cgactacggc gcccgcgacg tcgatcttct tccggttcgt ccgcgcgccg
      901 ctcgtgggct cgaggatggt gatctgcggc ccggaggcgc ggacggtgac ctggcgcgtc
      961 gcggaggccg tccggccggc cgcgtccgcc gcgatggcga cgatctcgct cgggacgtcc
     1021 ggcgggagga cgaggtccgg cagcgaccac gtggcaccag tcagcgtcgc cgccctccca
     1081 ttcacggtca cggacgtgac ggaggcgccg gccgtcccgg tcacggccac ggtcggcccg
     1141 ggcaggaccg cgcggtcggc cggcgcggtg atgtccacgg ccggtcccac gctgaggtcc
     1201 accttgaagg aagcgctcgc ggaggacgag tggccgagag cgtcggtggc cgtcgcgttg
     1261 agcgtgtagt cgccggaagc cgagatcgac gttccggaga ggaaggagac cccgttgaga
     1321 gtggccagga ccgtggccgt ggggtcgttc gagcggacga ctggcgcgac cgaccggaag
     1381 aaacgcgcgc ccacacccag cggcgcaccc gactcgagga tttcgacgga cggcgcgagc
     1441 gtcttcacgt tgaaggagcg cgagaaggtc ccgcggtttc cggcgcgatc gacgaggacg
     1501 atcgaggctg tgttggcgcc ttccttcgaa gagaaaggag tcgccacgaa cgtcccgccg
     1561 ctcacggtgg cggccgctcc gttgacggtc gcggcggcga ggtccggatc ggacgttccg
     1621 ctgaaggacg cgaccgcagc gccgagcgcc gcgccgtcgg ccggcgacgt cgtgaggagc
     1681 cgcggcggcg tgacgtcgat cgtgaagagc gcgtgcgcgg agacggaatg gcccgcgcaa
     1741 tcggtcacag tcgcggcgag gaggtgcgtg ccttccgcgg agatcggcgt gccggtccac
     1801 ggcgcgccgt ccagcgtgac ggacggcgcg ggcggcgcgg agccgggccc gtccgaaacg
     1861 gcgacgcgcg gcgagagcgc gcggccgaag agaacg
//
LOCUS       DAOMFY010000475         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_33603_length_1861_cov_6.650609, whole genome shotgun sequence.
ACCESSION   DAOMFY010000475 DAOMFY010000000
VERSION     DAOMFY010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33603_length_1861_cov_6.650609"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLB01_20140"
     CDS             <1..607
                     /locus_tag="PLB01_20140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ79664.1"
                     /translation="LELLEEQLRDAARTGGPPPADLAQAARQETTRLSRLLADFLSYA
                     RPSPLSTVPADLNEAAGDAVAFLAPEAERRAIRLAFTPNPGGAPSRLDPARVRQVVLN
                     LVANALDAVEAEGASAREVDVRVEDAGAKWRLTVADRGPGVPHAKTADLFKLFVSTKP
                     AGTGLGLPIAERIVKAHGGTLTLTSRPGEPTRAEATFPKNA"
     gene            complement(609..1076)
                     /locus_tag="PLB01_20145"
     CDS             complement(609..1076)
                     /locus_tag="PLB01_20145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQQ79665.1"
                     /translation="MSHRAFSVAFEVRFPEVDSYGVVWHGHYVQYFEVARNALCAAFG
                     LTPADALAHGYKVPITKVSLDLKRPARLDDRLAVTARLAPPETAKLTMSYEIRRETTG
                     ELLAAGSTEQVILDPSGQLLLTFPAPVKTLVARILAFQRGEAEPDASKILPPA"
     gene            complement(1073..>1861)
                     /locus_tag="PLB01_20150"
     CDS             complement(1073..>1861)
                     /locus_tag="PLB01_20150"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQQ79666.1"
                     /translation="TGSAGAGPLTGLERQMAQSPESASPFLFPNTVANAPASQAAIEI
                     GIKGPNVTITQKDPAPLNALFYGRMLLADGRADALLVGASDEWNLDYHLAYERVHATR
                     TATRPGFVLGEGAAALLVEDEDAARARGARPFARIASVVSRGAPVSPQRRRADPATLA
                     AVMNAALAEAGVSPRDVGLVHLSKNGVPATDAAEEAALAAVFGLAQPPAVAIKHALGE
                     NPAIGAVQLALAADALRTAPSLGAVLVNAFGAGGNFLAAVLTAT"
BASE COUNT          244 a          648 c          736 g          233 t
ORIGIN      
        1 cctcgagctc ctcgaggagc agctgcgcga cgccgcccgc acgggcgggc cgccgccggc
       61 cgacctcgcg caggccgcgc ggcaggagac gacgcgcctg tcgcgtctcc tcgcggactt
      121 cctgtcgtac gcgcgcccgt cgccgctgag cacggtgccc gcggacctca acgaggcggc
      181 gggagacgcg gtcgccttcc tcgcgccaga ggcggagcgc cgcgccatcc ggctcgcgtt
      241 cacgcccaac ccgggcggcg cgccgtcgcg tctcgatccc gcacgcgttc ggcaggtcgt
      301 cctgaacctc gtcgcgaacg cactcgacgc cgtggaggcc gagggcgcct cggcgcgcga
      361 ggtggacgtg cgcgtcgagg acgccggggc gaagtggcgc ctgacggtcg ccgaccgcgg
      421 gccgggcgtc ccgcacgcga agaccgcgga tctcttcaag ctcttcgttt cgacgaagcc
      481 cgcggggacg gggctcggcc tcccgatcgc cgagcgcatc gtgaaggcgc acggcggcac
      541 gctgacgctc acgtcgcgcc ccggggagcc gacacgcgcc gaggcgacgt ttccgaagaa
      601 cgcctgactc aggccggcgg gaggatcttc gacgcgtccg gctccgcctc gccgcgctgg
      661 aacgcgagaa tgcgcgcgac gagcgtcttc acgggcgcgg gaaacgtcag caggagctgt
      721 ccggacgggt cgaggatcac ctgctccgtg cttcccgccg cgaggagctc gccggtcgtc
      781 tcccgccgga tctcgtacga catcgtgagc ttcgccgtct cgggcggcgc gaggcgggcg
      841 gtgaccgcga ggcggtcgtc gagccgggcg ggccgcttga ggtccagaga caccttcgtg
      901 atcggcacct tgtagccgtg cgcgagcgcg tcggccggcg tgaggccgaa cgcggcgcac
      961 agcgcgttgc gcgcgacctc gaagtactgg acgtagtggc cgtgccagac gacgccgtac
     1021 gagtcgacct cgggaaagcg gacctcgaac gcgacggaga aagcccggtg gctcatgtcg
     1081 cggtcaggac ggccgcgagg aagttgccgc cggcgccgaa cgcgttcacg aggacggcgc
     1141 cgagggacgg tgccgtgcgg agggcgtcgg ccgcgagggc gagctggacg gcgccgatcg
     1201 ccgggttctc tccgagcgcg tgcttgatcg cgacggcggg cggctgcgcg aggccgaaga
     1261 cggcggcgag cgccgcttcc tcggcggcgt cggttgccgg aacgccgttc ttcgagaggt
     1321 ggacgagccc gacgtcgcgg ggcgacacgc cggcctcggc gagcgcggcg ttcatgaccg
     1381 ccgcgagcgt cgcgggatcc gcgcgccggc gctgaggcga gacgggcgcg ccgcgggaca
     1441 cgacggaggc gatgcgcgcg aagggccggg ccccgcgggc gcgggcggcg tcctcgtcct
     1501 cgacgagaag cgcggcggct ccctcgccga ggacgaaccc cgggcgcgtg gccgtgcgag
     1561 tcgcgtggac gcgctcgtag gcgaggtggt agtcgaggtt ccactcgtcc gaggcgccca
     1621 cgaggagggc gtcggcgcgg ccgtccgcga gcagcatgcg tccgtagaag agcgcgttga
     1681 gcggtgcggg gtccttctgg gtgatcgtga cgttcgggcc cttgatgccg atctcgatcg
     1741 cggcctgcga cgcgggtgcg ttcgcgaccg tgttcgggaa gaggaacggc gacgcggact
     1801 ccggcgactg ggccatttgc cgctcgagcc ccgtcagcgg gccggcgccg gccgagccgg
     1861 t
//
LOCUS       DAOMFY010000476         1854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_33836_length_1854_cov_5.716509, whole genome shotgun sequence.
ACCESSION   DAOMFY010000476 DAOMFY010000000
VERSION     DAOMFY010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33836_length_1854_cov_5.716509"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLB01_20155"
     CDS             complement(<1..189)
                     /locus_tag="PLB01_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011364420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQQ79667.1"
                     /translation="MDPARVPALARRVADAGGAVPFEAWMDLALHDAEAGYYARHIRD
                     VGARGDFATASTLHPALGE"
     gene            262..1152
                     /locus_tag="PLB01_20160"
     CDS             262..1152
                     /locus_tag="PLB01_20160"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQQ79668.1"
                     /translation="MRKPSPDWVNILFLCLTPVVGFGGTAWYAATQGIVWWEPVLALA
                     LFQAIGIGIGSGYHRCFAHRTYDCHPAAECVLLFFGALALQNSALRWARDHRDHHRYT
                     DTDLDPYSVTRGFLWAHVFWVFYKQPPDASFDNVPDLLKNPRVMWQHRWSRFLGVGVG
                     LGLPTLVGAFFGRPLGGLLWGGFLRIVLVHHTTFLINSAAHLWGKRPYSTEVSARDNW
                     FLAFFTHGEGFHNFHHTFPSDFRNGLRWYHWDPNKWAILVLRVTGLARNLRRTPGPLI
                     ERARERVLEAASGLSTSPTT"
     gene            1247..1322
                     /locus_tag="PLB01_20165"
     tRNA            1247..1322
                     /locus_tag="PLB01_20165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1280..1282,aa:Ala,seq:ggc)
     gene            1380..1607
                     /locus_tag="PLB01_20170"
     CDS             1380..1607
                     /locus_tag="PLB01_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79669.1"
                     /translation="MKSPKTQARTRTTRGSRDLKAALKRAAAALEKAAMAAPRSRGSR
                     DLRSEIARAKKAVLKATAIAHTTRGSRDLKG"
BASE COUNT          271 a          692 c          623 g          268 t
ORIGIN      
        1 ctcgccgagc gcggggtgca gcgtcgaggc cgtcgcgaag tcgccgcgcg cgcccacgtc
       61 ccggatgtgg cgggcgtagt agccggcctc cgcgtcgtgc agcgcgaggt ccatccacgc
      121 ctcgaacggc acggcgccgc cggcgtcggc gacgcggcgg gccagggccg gaacgcgggc
      181 ggggtccacg ccccatgatc cgacacgccc gtccccgcgg acgagacgcc gtcgcagaag
      241 aacgcgtaaa ctccacgtcc tatgcgtaaa ccctcgccgg actgggtgaa catcctcttc
      301 ctctgcctca cgcccgtggt cggcttcggc ggcaccgcct ggtacgccgc cactcaggga
      361 atcgtctggt gggagccggt cctcgcgctc gcgctcttcc aggcgatcgg catcgggatc
      421 ggctcgggct accaccgctg cttcgcccac cgcacgtacg actgccaccc ggcggcggag
      481 tgcgtcctgc tcttcttcgg ggcgctcgcg ctccagaact ccgcgctccg ctgggcgcgc
      541 gaccaccgcg accaccaccg ctacacggac acggacctcg acccgtactc cgtgacgcgc
      601 ggattcctct gggcgcacgt cttctgggtg ttctacaagc agcctccgga cgcgagcttc
      661 gacaacgtgc ccgacctcct gaagaacccg cgcgtcatgt ggcagcaccg ctggtcgcgc
      721 ttcctcgggg tcggcgtcgg cctcggcttg ccgacgctcg tcggcgcgtt cttcgggcgg
      781 cccctcggcg gccttctctg gggcgggttc ctccggatcg tcctcgtcca ccacacgacg
      841 ttcctcatca actccgcggc gcatctctgg ggcaagcggc cgtactccac cgaggtctcg
      901 gcgcgcgaca actggttcct cgcgttcttc acgcacggcg aagggttcca caacttccac
      961 cacacgttcc cgtccgactt ccgcaacggt ctccgctggt accactggga tccgaacaag
     1021 tgggcgatcc tcgtcctcag ggtcaccggt ctcgcgcgga acctccggcg gactccaggg
     1081 ccgctcatcg agcgggcgcg cgagcgtgtc ctcgaagcgg cgtcggggct ctcgacgagc
     1141 ccgaccacct gaagccgacc gcccccgcgc ccgtcgaagc tccgaattcc cgcgtttgac
     1201 cgggagcgga ggtggtgcta ctttccccgg tctctccgac ccgggtgggg ctgtagctca
     1261 gttgggagag cgcctgaatg gcattcagga ggtcgacggt tcgatcccgt tcagctccac
     1321 cactttagat aacctacccc gcgcgacgta cagtgaaacg gcatgcgggg aggacgcacg
     1381 tgaaaagccc gaaaacgcag gctcggacgc gcacgacgcg cggctcgcgt gacctgaagg
     1441 ccgcgctgaa gagagcggcg gccgcgctcg agaaggcggc catggccgcg ccgaggagcc
     1501 gcggctcgcg cgacctgcgg agcgagatcg cgcgcgcgaa gaaggccgtg ctgaaggcga
     1561 cggcgatcgc gcacacgaca cgcggctcgc gcgacctgaa gggctgaccc tctcatccgg
     1621 cccgctggtc acggcgcgtg cgagagcaag cgtgcggcga acgcaccctc gcgcacgcgc
     1681 cgcgcccgct accggctagg caggaggctt cgccgatgcc gaggacgcgc ccgaagagca
     1741 cgacgaggga gaaatgcccc cggcggctcc ggcgagaggg ctggcggcgc cgggcgcggg
     1801 gcagcgcgcg cgccgggtcc ctcttccccg gcccttctcc cggcacgcgc gctg
//
LOCUS       DAOMFY010000477         1841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_34240_length_1841_cov_5.193729, whole genome shotgun sequence.
ACCESSION   DAOMFY010000477 DAOMFY010000000
VERSION     DAOMFY010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34240_length_1841_cov_5.193729"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /gene="mnmG"
                     /locus_tag="PLB01_20175"
     CDS             <1..1803
                     /gene="mnmG"
                     /locus_tag="PLB01_20175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQQ79670.1"
                     /translation="PATIGRMSCNPAVGGLAKGQVMREIDALGGAMGLVADRTAIQFK
                     VLNASRGPAVRGLRCQSDKVLYAAELRRVVESAPRVTVAAGTAAGFAVEKGRLAGIVL
                     EDGRTLPCRAAVVTTGTFLSGLVHVGESRRESGRWDEPPAKSLSGALSALGLRLGRMK
                     TGTPPRVRAGSVDVSRMDRAAGDAQPVPFSFRSRGGTFPVQPQVDCWLTHTGERVHDL
                     IRGSLPLSPLYSGRITGRGPRYCPSIEDKVVRFPDKARHQIFVEPEGLSTDWLYLNGL
                     SMSLPPATQEQIVRLINGLERAEILRPAYAVEYDVVFPEQLDVSLETRALPGLFLAGQ
                     INGTSGYEEAAGQGLVAGANAARSAKDLGEALVLSRDEAYVGVMLDDLVTLGLDEPYR
                     LLTSRAEHRLLLGADSAYARLTEKAVAFGLVAPEDAGPILEAEARHARVSAALEAARV
                     TPDRATRARLAEEGVDVGEETSLAGLLRRPDLDVASLRTFLAETLGETARDDLAALDA
                     EALDRVASELRYAGFLARERQTVARLKRAGGRRIPRDFVYRGVPGLSLEAVEKLERHR
                     PGTLGQAARIPGVTAAAAALLLARLLARDRSAAA"
BASE COUNT          241 a          653 c          689 g          258 t
ORIGIN      
        1 cccgcgacga tcgggcgcat gagctgcaat ccggcggtcg gcgggctcgc aaagggccag
       61 gtcatgcgcg agatcgacgc gctcggcggc gcgatgggcc tcgtcgcgga ccgcacggcg
      121 atccagttca aggtgctgaa cgcgtcgcga gggccggccg tgcgcggcct gcgctgccag
      181 tcggacaagg tgctctacgc ggcggagctg cgccgcgtcg tggagtcggc cccgcgcgtc
      241 acggtggccg ccggaacggc ggcggggttc gcggtggaaa aggggaggct cgcgggcatc
      301 gtcctcgaag acggccggac gctcccgtgc cgcgccgccg tcgtgacgac ggggactttt
      361 ctttcgggcc tcgtgcacgt gggggagagc cgccgcgagt cggggcggtg ggacgagccg
      421 ccggcgaagt ctctttccgg cgccctctcg gcgctcggcc tccggctcgg gcgcatgaag
      481 acggggacgc cgccgcgcgt ccgcgcggga agcgtggacg tttcgcggat ggatcgcgcg
      541 gcgggcgacg cgcagcccgt gccgttctcg ttccgttcgc gcggcgggac gttccccgta
      601 cagccgcagg tggactgctg gctcacgcac accggcgagc gcgtgcacga cctcatccgc
      661 gggagcctgc cgctctcacc gctctactcg ggacggatca cgggccgcgg gccgcggtac
      721 tgcccgtcca tcgaggacaa ggtcgtgcgg ttccccgaca aggcgcgcca ccagatcttc
      781 gtcgagcccg agggcctctc gacagactgg ctctatctga acggtctttc gatgtcgctg
      841 ccgcccgcga cacaagaaca aatcgtacga ttgattaatg gcctggagcg ggccgagatc
      901 cttcgccccg cgtacgcggt cgagtacgat gtcgtattcc cggaacagct ggacgtgtcg
      961 ctcgagacgc gcgcgctccc ggggctcttc ctcgcaggcc agatcaacgg aacgtccggc
     1021 tacgaagagg cggcgggcca ggggctcgtc gcgggcgcga acgcggcgcg ttcggcgaag
     1081 gacctcggcg aggctctcgt tctttcgcga gacgaggcct acgtcggcgt gatgctggac
     1141 gacctcgtga cgctcggcct cgacgagccc taccggctcc tcacgtcgcg cgccgagcac
     1201 cggctcctcc tcggcgccga cagtgcgtac gcgcgcctca cggagaaggc ggtcgcgttc
     1261 gggctcgtcg cgccggaaga cgccgggccg attctcgagg ccgaggcgcg ccacgcccgc
     1321 gtgtccgcgg cgctcgaagc ggcgcgggtc acgccggacc gcgcgacgcg cgcccgcctc
     1381 gccgaggagg gcgtcgacgt cggcgaggag acgtcgctgg cgggactgct ccgtcgcccg
     1441 gacctcgacg tcgcctcgct gcgaacgttt cttgccgaaa cgctcggcga aacggcgaga
     1501 gacgacctcg ccgcgctcga cgccgaggcg ctggaccgcg tcgcctccga gctccggtac
     1561 gcgggcttcc tcgcgcgcga gcggcagacc gttgctcggc tgaagcgcgc gggcgggcgg
     1621 cgcatccccc gcgacttcgt ctaccgcggc gttccggggc tttcgctcga agcggtggaa
     1681 aagctcgagc ggcatcggcc gggcacgctc ggacaggccg cgcgcatccc cggcgtgacc
     1741 gcggcggctg cggcgctcct tctcgcgcgg cttctggcgc gggaccggag cgcggccgcg
     1801 tgaaagcggc cgcagacagc cgactgcgcg ccgccgtggc g
//
LOCUS       DAOMFY010000478         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_34615_length_1829_cov_6.372041, whole genome shotgun sequence.
ACCESSION   DAOMFY010000478 DAOMFY010000000
VERSION     DAOMFY010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34615_length_1829_cov_6.372041"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLB01_20180"
     CDS             complement(<1..1158)
                     /locus_tag="PLB01_20180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQQ79671.1"
                     /translation="MTRHLLPPLVLRPLAKDRLRFALTVSGIAVGVATMAAIRLANAS
                     VLSSFSETVDFVAGKASITILADGPGIPEETLERLAWLRGIGATLAPAITETAAAGAK
                     DGEVVEVLGIDPLADANAREYRFADEGKREEIFSIFEEESLLITSAFAERHRLKAGDA
                     FTLVTNSVERTFRVAAVLRPTGAARAASGSIVFMDIAAAQEAFGKVGRLDRIDVVLPA
                     PLSEADRARVLEEVSASLPPGVTAGRPERRTETVDRMVRAFRVNLSALGAIALLVGMY
                     FVYNTLSISVLRRRADIGVVRALGASRRSVFAAFLLEGLSLGVAGSVLGLALGAALAA
                     GALKLVGGTATELYVPSAHPVLRLDPGVLAFAFALGVATSVLSALAPALEAA"
     gene            complement(1155..>1829)
                     /locus_tag="PLB01_20185"
     CDS             complement(1155..>1829)
                     /locus_tag="PLB01_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ79672.1"
                     /translation="ARVAAESVSKSYLPGPGASPVDALADVSLELSAGSFTALLGPSG
                     CGKSTLLNLLGGLDRPTAGRVLVDGRDLATLREDERDTYRRRTAATVFQFFNLLPTMN
                     ALENVALPLLLDGVAPSGADARAASLLAEVGLSGKERAYPYELSGGQMQRVAVARALA
                     NRPALLLADEPTGNLDSKTGAQVLDLISGLVATHGITVVMATHSAGAAARASRVLNLL
                     DGRLVP"
BASE COUNT          281 a          630 c          707 g          211 t
ORIGIN      
        1 ggccgcctcg agcgcgggcg cgagcgcgga gagcaccgac gtcgcgacgc cgagggcgaa
       61 cgcgaacgcg aggacgccgg ggtcgaggcg cagcacgggg tgggcggagg ggacgtagag
      121 ctccgtcgcc gtgccgccca cgagcttcaa cgcgcccgcg gcgagggccg cgccgagcgc
      181 gaggccgagg acgctgccgg cgacgccgag cgagagtcct tcgaggagga aggcggcgaa
      241 gacggagcgg cgcgacgcgc cgagggcgcg cacgacgccg atgtccgcgc ggcggcgcag
      301 gaccgagatc gagagcgtgt tgtagacgaa gtacatcccg acgaggagcg cgatcgcgcc
      361 gagcgccgag aggttcacgc ggaacgcccg caccatgcgg tccaccgtct ccgtccgccg
      421 ctcgggccgg ccggccgtca cgccgggcgg gagcgaggcg gagacctcct cgaggacgcg
      481 ggcgcgatcg gcctcggaga gcggcgcggg caggacgacg tcgatccggt cgaggcgccc
      541 gaccttgccg aacgcctcct gcgcggcggc gatgtccatg aagacgatcg acccggacgc
      601 cgcgcgcgcg gccccggtcg gccgcagcac ggcggcgaca cgaaacgtgc gctccacgga
      661 gttcgtgacg agcgtgaagg cgtcgcctgc cttcagccga tgccgctcgg cgaaagcgct
      721 ggtgatgaga agagattctt cttcgaatat ggagaagatt tcttctcttt tcccctcgtc
      781 agcaaatctg tattcccgcg cgttcgcgtc cgcgagcggg tcgatgccga ggacttcgac
      841 gacctcgccg tccttcgcgc ccgcggccgc cgtctccgtg atcgccggcg cgagcgtcgc
      901 gccgatcccg cgcagccacg cgaggcgctc gagcgtctcc tccgggattc ccgggccgtc
      961 cgcgaggatc gtgatcgacg ccttccccgc cacgaagtcc accgtctccg agaacgacga
     1021 aaggaccgat gcgttcgcga ggcggatcgc cgccatcgtc gcgacgccga ccgcgatgcc
     1081 ggaaaccgtg agcgcgaagc gcaggcggtc cttcgcgagg gggcgcagga cgagcggcgg
     1141 aaggaggtgg cgcgtcacgg cacgagccgc ccgtcgagga ggttgaggac gcgcgaggcg
     1201 cgcgccgcgg ctccggccga gtgcgtcgcc atcacgaccg tgatcccgtg ggtcgcgacg
     1261 agccccgaga tcaggtcgag gacctgagcg cccgtcttcg agtcgaggtt gccggtcggt
     1321 tcgtccgcga ggaggagcgc cgggcggttc gcgagggcgc gcgcgaccgc gacgcgctgc
     1381 atctggccgc ccgagagctc gtacgggtac gcgcgctcct tgccggagag cccgacctcc
     1441 gcgaggagcg acgcggcgcg cgcgtcggcc ccggacggcg cgacgccgtc gaggagaagc
     1501 ggcagggcga cgttctcgag cgcgttcatc gtcggcagga ggttgaagaa ctggaagacc
     1561 gtcgccgcgg tccgccggcg gtacgtgtcg cgctcgtcct cgcgaagcgt cgcgaggtcc
     1621 cgcccgtcca cgaggacccg gcccgccgtg ggccggtcca gtccgccgag gaggttcagg
     1681 agcgtcgact tgccgcagcc ggagggtccg aggagcgccg tgaagctccc ggcagaaagc
     1741 tcgagcgaga cgtccgcgag cgcgtccacg ggagacgcgc ccggccccgg caggtaggac
     1801 ttggagacgg actcggcggc gacgcgggc
//
LOCUS       DAOMFY010000479         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_34961_length_1820_cov_6.241360, whole genome shotgun sequence.
ACCESSION   DAOMFY010000479 DAOMFY010000000
VERSION     DAOMFY010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34961_length_1820_cov_6.241360"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLB01_20190"
     CDS             complement(<1..485)
                     /locus_tag="PLB01_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79673.1"
                     /translation="MSARRFAPGPALAAALLLAAAALLTHRDLVTGASTPAYRDIGTT
                     QRPARALAATLGAAKLNPAASFGQAYRGNPNLVLAYPFPAAPRWLGVQLLLHLALGLL
                     GAFLFLRAEGRSKDAALVGAFAWGLSGFVLSSAAFLNAATTLAWAPWLLYRLDEHYEW
                     LG"
     gene            complement(482..1225)
                     /locus_tag="PLB01_20195"
     CDS             complement(482..1225)
                     /locus_tag="PLB01_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ79674.1"
                     /translation="MPVFGKRVAVVMPAYNAEKTLHAAFHEIPREWVDDVILVDDGSK
                     DRTVEVARSIPGVHVVAHERNKGYGGNQKTCYATALARGADICVMVHPDHQYDASVLP
                     DLVRPIAEGLEDAMFGSRMLGGRPLEGGMPKWKYLANIFLTAVENATFYLYLTEYHSG
                     LRAYSRRYLESVNLSAFSDDFVFDTEIIAAGIWKGMRIREVPIVTRYFDDASQISFRR
                     SCVYGLSILWVMARFKMNKWGWRKDPLFA"
     gene            complement(1235..>1820)
                     /locus_tag="PLB01_20200"
     CDS             complement(1235..>1820)
                     /locus_tag="PLB01_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79675.1"
                     /translation="WARALAPIALASALVVLPGAPYLSRAFAYASAPGDDVLELFARL
                     RALDPPPVDPAALPPPAPGSIPGVMPPWSAGHFVTALAGRPSAADPFVYGWRRQCRLF
                     TATDDAEALAILRAAKCRYLVTADVSRLLPAYAAAAGRAPGPTSSMFGKRVHESLAER
                     PVPFLVRVLDSRTGSVRPDGSFQPRFRVFRVDSP"
BASE COUNT          298 a          602 c          704 g          216 t
ORIGIN      
        1 ccgagccact cgtagtgctc gtcgaggcgg tagaggagcc acggcgccca cgcgagcgtc
       61 gtcgcggcgt tcaggaacgc ggccgaggag agcacgaaac ccgacaggcc ccacgcgaag
      121 gctccgacga gcgccgcgtc cttcgagcgc ccctcggcgc gcaggaacag gaacgcgccg
      181 aggagcccga gggcgaggtg gaggaggagc tggacgccca gccaccgggg cgcggccggg
      241 aacgggtacg cgaggacgag gttcgggttc ccgcggtacg cctgcccgaa ggaggccgcc
      301 gggttgagct tcgcggcgcc gagcgtcgcc gcgagggcgc gggcgggacg ctgggtcgtg
      361 ccgatgtcac ggtacgcagg cgtcgacgcg cccgtgacga ggtcgcggtg cgtcaggagc
      421 gcggcggccg cgaggaggag cgccgccgcg agcgcgggcc ccggtgcgaa acgccgcgcg
      481 ctcacgcgaa gagggggtct tttcgccagc cccacttgtt catcttgaac cgcgccatca
      541 cccacaggat cgacaggccg tagacgcacg accgccggaa gctgatctgc gaggcgtcgt
      601 cgaaatagcg cgtcacgatc gggacctcgc ggatgcgcat gcccttccag atcccggccg
      661 cgatgatctc cgtgtcgaac acgaagtcgt cggaaaacgc ggagaggttc acgctctcga
      721 ggtagcgccg cgagtaggcg cgcagcccgg agtggtactc cgtgaggtag aggtagaacg
      781 tcgcgttctc gaccgcggtc aggaagatgt tcgcgaggta cttccacttc ggcatgccgc
      841 cctcgagggg ccgcccgccc agcatccggc tcccgaacat cgcgtcctcg agcccctcgg
      901 cgatcggacg cacgaggtcg ggcaggacgg acgcgtcgta ctggtggtcg gggtggacca
      961 tcacgcagat gtccgccccg cgcgcgagcg ccgtggcgta gcaggtcttc tggttcccgc
     1021 cgtagccctt gttccgctcg tgggcgacga cgtggacgcc gggaatcgag cgtgcgacct
     1081 cgaccgtgcg atccttcgag ccgtcgtcca cgaggatgac gtcgtcgacc cactccctcg
     1141 ggatctcgtg aaaggccgcg tgcagcgtct tttccgcgtt gtaggcgggc atcacgaccg
     1201 cgacgcgctt gccgaaaacg ggcacgggag gattctaggg ggagtcgacg cggaaaaccc
     1261 ggaagcgcgg ctggaaactg ccgtccggcc ggacggagcc cgtgcgcgaa tcgaggacgc
     1321 ggacgaggaa cgggacgggc cgctcggcca gggactcgtg cacgcgcttg ccgaacatcg
     1381 acgacgtcgg gcctggagcg cgccccgccg cggcggcgta ggccggcagg aggcgcgaca
     1441 cgtccgccgt gacgagatag cggcacttcg cagcgcggag aatcgcgagc gcttcggcgt
     1501 cgtcggtcgc cgtgaagagc cggcactggc gccgccagcc gtagacgaac gggtcggcgg
     1561 cggacgggcg tccggcgaga gccgtcacga agtggcccgc cgaccacggc ggcatcacgc
     1621 cggggatcga gcccggcgcc ggcggtggca gcgcggcggg atccacgggc ggcgggtcga
     1681 gcgcccggag gcgcgcgaag agctcgagga cgtcgtcgcc gggagcactc gcgtaggcga
     1741 acgcgcgcga gaggtacggc gcgcccggaa gcacgacgag ggccgaggcg agggcgatcg
     1801 gtgcgagcgc gcgcgcccag
//
LOCUS       DAOMFY010000480         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_35029_length_1818_cov_5.402723, whole genome shotgun sequence.
ACCESSION   DAOMFY010000480 DAOMFY010000000
VERSION     DAOMFY010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35029_length_1818_cov_5.402723"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1261)
                     /locus_tag="PLB01_20205"
     CDS             complement(80..1261)
                     /locus_tag="PLB01_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQQ79676.1"
                     /translation="MHINDLLKISVERKASDLHLKVGAHPIIRVDGELIPLVDLKRLM
                     QEDTIAMAFSIMSARQKEKFKNNFEIDIAYSVPGLGRFRCNIFQQRGTVGLVCRVIPA
                     RILTIRDLLLPPVLEKICEETRGLILVTGTTGSGKSTSLAAMIDYINSTRAEHIMTIE
                     DPVEFLHRDKKSIINQRELEVDTRSFASAIRAALRQDPDVILVGEMRDFETVETALVA
                     AETGHLVMSTLHTMDATETVNRIISVFPPHQQKQIRLQLAAVIKAVCSMRLLPRADGL
                     GRVPAVEVMIATNYIRDCIENKEKTKYITDAIKSGTSQYGMQTFDQSLFQLYKTGLIT
                     LEEAIRRSTNPNEFKLKIQGIQSTSDIANELMEGSLQAPDGVGPRDSDSPFDFSNQST
                     G"
     gene            1384..>1818
                     /locus_tag="PLB01_20210"
     CDS             1384..>1818
                     /locus_tag="PLB01_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQQ79677.1"
                     /translation="MADEKDHPNRGGGFTRRGLIKGLATTAVATATAGATALAEERAK
                     AETAAGGGLPVSLTLNGAVKKGVFEPRTTLAEALRDAWGMTGTKVGCDRGACGACTVL
                     LDGLAVNSCLTLVHDADGRKVTTIEGLGSPEAMSPIQKAFVDA"
BASE COUNT          286 a          566 c          647 g          319 t
ORIGIN      
        1 cgcggcgagc caagagggcg agagccgtgt ttctcggatc ggggggagct tccccgctcc
       61 ccccggggcc ccccatgggt taccccgtcg actggttcga gaagtcgaag ggcgagtccg
      121 agtcccttgg cccgactccg tccggcgcct gcagcgaacc ttccatgagc tcgttcgcga
      181 tgtccgacgt cgactggatg ccctggatct tgagcttgaa ttcgttggga ttcgtcgagc
      241 ggcggatcgc ctcttccagc gtgatgaggc cggtcttgta gagctggaag agcgactggt
      301 cgaacgtctg catgccgtac tgcgaggtgc cggacttgat cgcgtcggtg atgtacttcg
      361 tcttttcctt gttctcgatg cagtcgcgga tgtagttcgt ggcgatcatg acctcgacgg
      421 ccggcacgcg gccgaggccg tcggcgcgcg ggaggaggcg catcgagcac acggccttga
      481 tgacggccgc gagctggagg cggatctgct tctgctggtg cggcgggaag accgagatga
      541 tgcggttgac ggtctcggtc gcgtccatcg tgtggagggt cgacatgacg aggtgtcccg
      601 tctcggcggc gacgagggcc gtctcgaccg tctcgaagtc gcgcatctcg cccacgagga
      661 tgacgtcggg atcctggcgg agggccgcgc ggatcgcgga cgcgaacgaa cgcgtgtcga
      721 cctccagctc gcgctggttg atgatcgact tcttgtcccg gtggaggaac tcgacggggt
      781 cctcgatcgt catgatgtgc tcggcccgcg tcgagttgat gtagtcgatc atcgcggcga
      841 gcgacgtcga cttgcccgag cccgtcgtcc cggtcacgag gatgaggccg cgcgtctcct
      901 cgcagatctt ctcgaggacg ggcgggagga gcaggtcgcg gatcgtcagg atgcgcgccg
      961 ggatgacgcg gcagacgaga ccgaccgtgc cgcgctgctg gaagatgttg cagcggaacc
     1021 gcccgaggcc cggcaccgaa tacgcgatgt cgatctcgaa gttgttcttg aacttttcct
     1081 tctgccgggc ggacatgatc gagaacgcca tcgcgatcgt gtcttcctgc atgaggcgct
     1141 tgaggtcgac cagcgggatc aactcgccgt ccacgcggat gatcgggtgg gcccccacct
     1201 tcaggtggag gtcggaggct ttccgctcca ccgagatctt gaggaggtcg ttgatgtgca
     1261 tcggtgccct cccgacgtgt ccgaaatccg ggcaaggata ggcttttcag gcacttgccg
     1321 tcaacggaag tgagccgttc ccaggccgcc ggctgtatag ttccgggcgc tcgaggaggg
     1381 gtcatggccg acgagaagga ccatccgaac cggggcggcg ggttcacacg ccgcggactc
     1441 atcaagggcc tcgcgacgac cgccgtcgcg accgccaccg cgggcgccac ggcgctcgcc
     1501 gaagagcgcg cgaaggccga gaccgccgcg ggaggcggac tccccgtgag cctcacgctc
     1561 aacggcgcgg tgaagaaggg cgtcttcgag ccgcggacga cgctcgcaga ggcgcttcgc
     1621 gacgcgtggg ggatgaccgg cacgaaagtc gggtgcgacc gcggcgcctg cggcgcctgc
     1681 acggtcctgc tcgacggcct cgccgtgaac tcctgcctca cgctcgtcca cgacgcggac
     1741 ggccggaagg tgacgacgat cgagggcctc ggctcgccgg aggcgatgtc tccgatccag
     1801 aaagccttcg tcgacgcc
//
LOCUS       DAOMFY010000481         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_35627_length_1802_cov_5.566686, whole genome shotgun sequence.
ACCESSION   DAOMFY010000481 DAOMFY010000000
VERSION     DAOMFY010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35627_length_1802_cov_5.566686"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..722)
                     /locus_tag="PLB01_20215"
     CDS             complement(63..722)
                     /locus_tag="PLB01_20215"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQQ79678.1"
                     /translation="MEKKRELMVETQIAARGIRDPRVLAAMRKVPRHLFVPAAEAASA
                     YDDRPLPIGSGQTISQPYVVAFMTEQLRLTGKEKVLEIGTGSGYQTAILAELAGKVYS
                     IEIRPELAAEAAARLKELGIGNVEVRAADGYRGWPEEAPFDGILVTAAPERIPPPLLE
                     QLASTGRMVIPVGAFYQELKVIERQGGGYTEKSVLPVRFVPFVGEAEGAPRPAPPGTD
                     R"
     gene            complement(816..>1802)
                     /locus_tag="PLB01_20220"
     CDS             complement(816..>1802)
                     /locus_tag="PLB01_20220"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HQQ79679.1"
                     /translation="TLYLRTDADGVTVASEALDDDPAWHRVPDGHLVTATAAGASVAL
                     PVDFGRPNLVRLSVRANGRPPEVMVLDSGASVSLLTESAAERLGVRFTQGATASARGL
                     HQTETPMRLGWLDSVRMGDLTITDVPVGVLPDGTLTFETASLGVFRLNGVLGAHLMKE
                     FDWRVEYIEKRLFARRVDPKAARGSKDQNMFFRRMKPMVRTSLNGQPWSLFLLDTGSE
                     PSMVTRGGLRKTRLFEPETAFPVTLEGIGNSRVSWGKISNVSLGVDAFMVRFKDIVVK
                     EEGDGLEDGILGASFLANFDVEIRFGPMTLALENPVERRRREARAPAQEVPR"
BASE COUNT          270 a          634 c          646 g          252 t
ORIGIN      
        1 ggacggggcg acgaggagca gagcggcgag cgccgcggcg agcggacggc ggagcggggc
       61 gctcagcggt ccgtcccggg cggggcgggg cggggcgcgc cttcggcctc gccgacgaag
      121 ggcacgaagc ggacgggcag gacgctcttc tccgtgtagc cgccgccctg acgttcgatg
      181 actttcaact cctggtagaa ggcgcccacg gggatcacca tgcgccccgt cgacgcgagc
      241 tgctcgagga gcggcggcgg gatgcgctcg ggtgcggccg tgacgagaat gccgtcgaac
      301 ggcgcctcct cgggccagcc ccggtagccg tccgcggcgc gcacctcgac gttcccgatg
      361 ccgagctcct tcagccgggc cgccgcctcg gcagcgagct cgggccggat ctcgatcgag
      421 tacaccttcc cggcgagctc ggcgaggatg gcggtctggt agcccgagcc ggtgccgatc
      481 tcgagcacct tctccttccc ggtcagccga agctgctcgg tcatgaaggc gacgacgtaa
      541 ggctgggaaa tcgtctgtcc gcttccgatc ggcagcggcc ggtcgtcgta ggcggaggcc
      601 gcctccgcgg cggggacgaa aagatgccgc gggaccttgc gcatcgcggc gagcacccgc
      661 gggtcccgga tgccgcgcgc cgcgatctgg gtctcgacca tgagttcgcg tttcttttcc
      721 attggatccg ccggagggcg gcacgcggca agaagggcga tccctcccag cgccaaagac
      781 acgagccccg tccgacgccc cctcacggga gcattctatc gagggacctc ctgcgcgggc
      841 gcgcgcgcct ctcgccgccg gcgctcgacc gggttctcga gcgcgagcgt catgggcccg
      901 aaacggatct cgacgtcgaa gttcgcgagg aacgaggcgc cgaggatccc gtcctcgagc
      961 ccgtcgccct cctccttcac cacgatgtcc ttgaaccgca ccatgaaggc gtccacgccg
     1021 aggctcacgt tcgagatctt tccccacgaa acgcgcgagt tgccgatccc ctcgagcgtc
     1081 accgggaacg cagtctccgg ctcgaagagc cgcgtcttgc ggaggccccc gcgcgtcacc
     1141 atcgaaggct ccgagcccgt gtcgaggagg aagagcgacc agggctggcc gttcagcgac
     1201 gtccggacca tgggcttcat gcggcggaag aacatgttct ggtccttcga gcctcgggcc
     1261 gccttcgggt ccacgcgccg ggcgaagagg cgcttctcga tgtactccac gcgccagtcg
     1321 aactccttca tgaggtgcgc gccgaggacg ccgttcagcc tgaagacgcc gagagatgcc
     1381 gtctcgaacg tgagcgtgcc atccgggagg acgcccaccg gcacgtccgt gatcgtgagg
     1441 tctcccatcc ggacggagtc gagccagccg aggcgcatcg gcgtctccgt ctggtgcagg
     1501 ccgcgcgccg acgccgtggc gccctgcgtg aaccgcacgc cgaggcgctc ggccgcgctc
     1561 tccgtcagga gcgagaccga ggcacccgag tcgaggacca tcacttcggg cggccgcccg
     1621 ttcgcgcgca cggaaagacg gacgaggttc ggccgcccga agtcgacggg cagcgccacg
     1681 ctcgcgccgg cggccgtggc ggtcacgagg tggccgtcgg ggacgcgatg ccacgcgggg
     1741 tcgtcgtcga gcgcctcgga cgcgacggtc acgccgtcgg cgtcggtgcg caggtacagg
     1801 gt
//
LOCUS       DAOMFY010000482         1797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_35826_length_1797_cov_6.373708, whole genome shotgun sequence.
ACCESSION   DAOMFY010000482 DAOMFY010000000
VERSION     DAOMFY010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35826_length_1797_cov_6.373708"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLB01_20225"
     CDS             <1..222
                     /locus_tag="PLB01_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79680.1"
                     /translation="LLAGVAAEVAGRARVLEVRLPRRGALHARLAGEMGFTEAAADTH
                     LVVRSFRPDVDAAAEGAAFDYRFLDHEVF"
     gene            complement(207..1214)
                     /locus_tag="PLB01_20230"
     CDS             complement(207..1214)
                     /locus_tag="PLB01_20230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQQ79681.1"
                     /translation="MKIVYVVPSLGISGGIKVVVEHAEGLAARGHDVSIVTLDARHAW
                     IPIHVPVVEVPEISRATLPEADVHVATWFPTVVPTARAGRARRVFHFSQGYEALYDFV
                     AHRREEIDEAYREPLPKLLISAHLRVLFEGVFPGPFHVLPQTIRAEDYRPPDPERATR
                     RNPPAVGVVGPFEAVKKGVAVALETVVRLRAEGRDVRLYRASQMRPSDAERRQILADV
                     YGEALPVDVMPIWYHACDVLLFPSYDAEGFGLPPLEAMASGVPAVISDIPSLAILPED
                     AVSRVPAGDAAAMARETARLLDDPRLWAARRAKGLEVARRFPLDPVLDRLEEIFSQKT
                     S"
     gene            complement(1211..>1797)
                     /locus_tag="PLB01_20235"
     CDS             complement(1211..>1797)
                     /locus_tag="PLB01_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79682.1"
                     /translation="TWAGGPGWRRLLAACGWRVETARPVTHVSRRLEPWVRALAEAGL
                     PEAETSAIQWLFLARADAPEGSLELGRSNVAFAALVEDPVGAARAAATASGEASWTLP
                     NAVSGAALEALFRGDVVLGEARAALSCGFTAAALARRFRGSGLAAAVTGLGRDALPPR
                     VAHALEAADRAGLRTDAEAVSSSRLGLMVRVEAR"
BASE COUNT          263 a          661 c          659 g          214 t
ORIGIN      
        1 ctcctcgcgg gtgtcgcggc cgaagtcgcg gggcgcgcgc gcgtcctcga ggtccgtctc
       61 ccccgccgcg gcgcgctcca cgcgcgcctc gcgggcgaga tggggttcac cgaggcggcg
      121 gccgacacgc acctcgtcgt gcgctcgttc cgcccggacg tggacgccgc cgccgaaggc
      181 gccgccttcg actatcgctt cctcgatcac gaagtcttct gagagaagat ttcctcgagc
      241 cggtcgagga cggggtccag cggaaacctg cgcgcgacct cgaggccctt cgcccgccgc
      301 gcggcccaga ggcgcgggtc gtcgagaagg cgcgccgtct cgcgggccat ggccgccgcg
      361 tcgccggccg ggacgcggga gaccgcgtcc tcgggaagga tcgcgagcga cggaatgtcg
      421 gagatgacgg cgggaacccc ggaggccatc gcctcgagcg gcgggagccc gaagccctcg
      481 gcgtcgtagg acgggaacag gaggacgtcg cacgcgtggt accagatcgg catcacgtcc
      541 acggggagcg cttctccgta gacgtccgcc aggatctgcc gccgttccgc atcggaggga
      601 cgcatctggc tcgcacggta cagccgcacg tcccggcctt cggcccgcag gcggaccacc
      661 gtctcgagcg cgaccgcgac ccccttcttc acggcctcga acgggcccac gacgccgacg
      721 gccggcggat tccggcgggt cgcgcgctca gggtccggcg gcctgtagtc ctccgcgcgg
      781 atcgtctgcg ggagaacgtg aaacggcccc gggaagactc cttcgaagag gacgcggagg
      841 tgggccgaga tgaggagctt cgggagaggc tcgcggtagg cctcgtcgat ctcctcgcgc
      901 cggtgtgcga cgaagtcgta gagcgcctcg taaccctggc tgaagtggaa aacgcgccgc
      961 gcccggccgg cgcgggccgt ggggacgacc gtcgggaacc acgtcgcgac gtggacgtcg
     1021 gcttcgggca gcgtcgcccg tgagatctcc ggcacctcga ccacgggaac gtgaatggga
     1081 atccacgcgt gacgcgcgtc gagcgtcacg atcgagacgt cgtggccgcg tgccgcgagc
     1141 ccctcggcgt gctcgacgac caccttgatg ccacccgaga tcccgaggct cgggacgacg
     1201 tagacgatct tcaccgtgcc tccacacgca ccatcagccc gaggcgggac gacgataccg
     1261 cttcggcgtc ggtccggagg ccggcgcgat ccgcggcctc gagcgcgtgt gcgacacggg
     1321 gagggagggc atcgcgtccc aggcccgtca cggccgcggc gagaccgctt ccgcggaacc
     1381 gccgcgcaag agccgccgcc gtgaacccgc aggagagcgc ggctcgcgcc tccccgagca
     1441 cgacgtcgcc cctgaaaagg gcctcgagcg cggcccccga aacggcgttc ggcagcgtcc
     1501 aggaggcttc gccggaggcc gtggccgcgg cgcgggccgc gcccacggga tcctcgacga
     1561 gcgcggcaaa cgcgacgttg gacctcccga gctcgagcga tccctcgggc gcgtccgcgc
     1621 gcgcgaggaa gagccactgg atcgccgacg tctcggcttc cggcaggccc gcctccgcga
     1681 gagcgcgcac ccagggctcc aggcgccgcg acacgtgcgt gaccggccgt gcggtctcga
     1741 cgcgccagcc gcaggcggcc aggaggcggc gccagccggg ccctcccgcc cacgtgc
//
LOCUS       DAOMFY010000483         1781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_36428_length_1781_cov_7.057937, whole genome shotgun sequence.
ACCESSION   DAOMFY010000483 DAOMFY010000000
VERSION     DAOMFY010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36428_length_1781_cov_7.057937"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1730
                     /locus_tag="PLB01_20240"
     CDS             219..1730
                     /locus_tag="PLB01_20240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79683.1"
                     /translation="MNLDRLRQKFFEGLTRYRWLETVGRGGMGIVFKAQDLDLEDVVA
                     IKVLSPDWETDDQQLLQRFKREINLNRKIKHPNVARIHDFGMSGDFPYITMEYVPGKD
                     LRTIIQTEGRLPQARAISILRQIALGSDAAHKLGIIHRDLKSQNVMLEDSGAVAILDF
                     GLARGKTNEQLTLDSVMVGTPHYMSPEQALGRPTDARSDVYSIGVMAYELLTGRVPFD
                     GESPLVIAMKHVSEPPPEDLRQMTDIAPELIAITYRALAKDPATRFASAADLEAELAM
                     LPPFREGAPVSGEAPALQTPAPPAPPSPSPGVPTPAVTQASAMLDLAGMTTPVRPTTA
                     VAASVVPTPPVAKNRPPVILLVEGDAKSRKALADVLGRHGCRTLEAKDGPEALERLLQ
                     DTVDLLLMDVQLPGMDGFDVTRVIKAQPQTAALPVVLTTARLDRSHFAFAIQSGATDV
                     IGKPLLNEAVVGRIWHILTHHGFLPPAGNEPVLAAMKKTLPSAMTPPSTPGKR"
BASE COUNT          305 a          677 c          562 g          237 t
ORIGIN      
        1 gtcggcggcc ggggcggtcc ccggggccgc cggggccgcc gccggccccg ccgccggctc
       61 gacgcccagg atcgacagga gggcgaggct cggaagaagg ttcattgaga ctccgacgca
      121 gtccgtgccg ggaagattac cccttcccgg aaaaaaacgg cacggcgccc gtcccgcccc
      181 cgaagttgct acgctacggc accggccccg ccgcaaccat gaatctcgac cgcctccgcc
      241 agaagttctt cgaagggctc acccgctacc ggtggctcga aaccgtcggg cgcggcggca
      301 tgggcatcgt cttcaaggcg caggacctcg acctcgaaga cgtcgtcgcg atcaaggtgc
      361 tttcgcccga ctgggagacg gacgaccagc agctcctcca gcggttcaag cgcgagatca
      421 acctcaaccg gaagatcaag caccccaacg tcgcccgcat ccacgacttc ggcatgagcg
      481 gcgacttccc ctacatcacg atggagtacg tcccgggcaa ggacctgcgg acgatcatcc
      541 agacggaagg ccgcctccca caggctcgcg cgatctcgat cctccgccag atcgccctcg
      601 ggagcgacgc ggcgcacaag ctgggaatca tccaccgcga cctgaagtcc cagaacgtca
      661 tgctcgagga ctcgggcgcc gtcgcaatcc tcgacttcgg cctcgcgcgc gggaagacga
      721 acgagcagct cacgctcgac agcgtgatgg tgggaacgcc gcactacatg tcgcccgagc
      781 aggcgctcgg ccgcccgacc gacgcacgca gcgacgtcta ctcgatcggc gtcatggcct
      841 acgaactcct cacgggccgc gtgccgttcg acggagaatc gccgctcgtc atcgcgatga
      901 agcacgtgtc cgaaccgccg cccgaagacc tccgccagat gacggacatc gcgccggagc
      961 tcatcgcgat cacctaccgg gcgctcgcaa aggatcccgc cacgcgcttc gcgtccgctg
     1021 cggacctcga ggccgagctc gcgatgcttc ctccgttccg cgagggcgcg cccgtctccg
     1081 gggaggcccc cgctctgcag acgcccgctc ctccggcccc tccgagcccg agcccgggcg
     1141 tgcccacgcc ggcggtcacg caggcgagcg cgatgctcga cctcgcgggc atgacgaccc
     1201 ccgtgcgccc gacgacggcc gtcgcggcca gcgtggtccc gacgccgccc gtcgcgaaga
     1261 atcgcccgcc cgtgatcctc ctcgtcgagg gcgacgcgaa gagccgaaag gccctcgcgg
     1321 acgtcctcgg gcgccatgga tgccggacgc tcgaggccaa ggacggcccc gaggcgctcg
     1381 agcggctgct ccaggacacc gtcgacctcc tcctcatgga cgtgcagctc cccgggatgg
     1441 acggcttcga cgtcacgcgc gtgatcaagg cgcagccgca gacagccgcc ctgcccgtcg
     1501 tcctcacgac cgcgcgcctc gaccgcagtc acttcgcgtt cgcgatccag tcgggcgcga
     1561 cggacgtgat cggcaagccc ctcctcaacg aggccgtcgt gggccggatc tggcacatcc
     1621 tcacgcacca cggcttcctg ccgcccgcgg ggaacgagcc ggtccttgcc gcgatgaaga
     1681 agacgctccc ctcggcgatg acgccgccct cgacgcccgg caagcgctag gtgtaaagac
     1741 tcaggaggtt gttcacccgg gttaaccggt ggaatgctgt t
//
LOCUS       DAOMFY010000484         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_36842_length_1770_cov_12.874636, whole genome shotgun
            sequence.
ACCESSION   DAOMFY010000484 DAOMFY010000000
VERSION     DAOMFY010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36842_length_1770_cov_12.874636"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..719)
                     /locus_tag="PLB01_20245"
     CDS             complement(144..719)
                     /locus_tag="PLB01_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79684.1"
                     /translation="MHKTKTAALALGLLLALVSPAFAEDTCTVKAVLGGKAVTMKHCA
                     VALYEDEHSVTLWFSDAPFTAAEVDMFHLNSYAKDKDEAGKPRTMMHMAFCPGAGKPE
                     AGPDAVKSVELSVNLASSVMVSRQWVFKLPAEKENLAFQKLAGTIDPGGRISGRATGG
                     KTSDGEKYSWDATFDLALPAKSAAAGPGCGN"
     gene            759..1214
                     /locus_tag="PLB01_20250"
     CDS             759..1214
                     /locus_tag="PLB01_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ79685.1"
                     /translation="MKRVLLYAASGGVLIAALQFIEYQHFVRVYPGEVYGGLVAVIFT
                     AVGAALGLRWTRAKKEIVVREVVREVRVRENGPFVLDADALRETGLTPREHEILGLIA
                     EGLSNREIGERLFVSENTVKTHSARVFEKLGASRRTQAVQEGRRRGLIP"
     gene            1326..>1770
                     /locus_tag="PLB01_20255"
     CDS             1326..>1770
                     /locus_tag="PLB01_20255"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HQQ79686.1"
                     /translation="MKKQVLKFGLICGAILGAMTAVMVPLCMNGTASFDNAEIFGYTI
                     QVLAFLLVFFAIRSYRESPGGGAITFGKAFQVGILVTLVAAAVYVVSWEIVYYNFIPD
                     FGDKYAAHMLEKMKTKGESAQAIEKATREMAKFKELYRNPLFNAGM"
BASE COUNT          296 a          551 c          622 g          301 t
ORIGIN      
        1 cgtggtggtc gtccgcgaac atcgcgaact ggcgctcgcc cacgcggaac gtgggcgcca
       61 tccacgcgac cttctcgtac gcgccgggga ggccgaggca gatggcccgc aggcggtcga
      121 gggtattcac ggccctgctg atctcagttt ccgcagcccg gccccgccgc cgccgacttc
      181 gcggggagcg cgaggtcgaa cgtcgcgtcc cacgagtact tttccccgtc gctcgtcttc
      241 ccgcccgtgg cgcggccgga aatcctcccg cccgggtcga tggtgccggc gagcttctgg
      301 aaggcgaggt tctccttctc cgcgggcagc ttgaagaccc actgacggct gaccatgacg
      361 gagctcgcga ggttgacgct cagctcgacg ctcttgacgg cgtccggacc cgcctcgggc
      421 ttgccggcgc ccgggcaaaa cgccatgtgc atcatcgtcc ggggcttgcc ggcctcgtcc
      481 ttgtccttcg cgtacgagtt gaggtggaac atgtcgacct cggcggcggt gaacggggcg
      541 tcggagaacc acagcgtcac gctgtgctcg tcctcgtaga gggccacggc acagtgcttc
      601 atcgtgacgg ccttcccgcc gaggaccgcc ttgacggtgc aggtgtcctc tgcgaaagcc
      661 ggagaaacga gggcgagaag gagtccgagg gccagggcgg cggtctttgt cttgtgcatg
      721 cgcggagcct atccccgggc tagactgatt ctcaacctgt gaagcgcgtc cttctctatg
      781 ccgcgagcgg cggcgtcctg atcgcggccc tgcagttcat cgagtaccag cacttcgtgc
      841 gcgtgtaccc cggtgaggtc tacggcgggc tcgtggccgt gatcttcacg gccgtggggg
      901 cggccctcgg cctgaggtgg acgcgggcga agaaggagat cgtggtccgg gaggtcgttc
      961 gggaggtccg ggtgcgcgag aacggaccgt tcgtcctgga cgcggacgca ctcagggaga
     1021 ccgggctgac gccgcgcgag cacgagatcc tcggcctcat cgccgagggg ctcagcaacc
     1081 gcgagatcgg ggaaaggctt ttcgtgtcgg agaacacagt gaagacgcac tcggcgcgcg
     1141 tcttcgagaa gctgggcgcc tctcgccgga cgcaggccgt ccaggagggc cggcggcggg
     1201 ggctgatccc gtgaaaacgg gtcgacgaaa gggtgaaatc cgcgcttctg atgcgaaatc
     1261 acccgaaggg atgacgacaa acgcccgccc gcggccgtag gttgccgggc gaaagggaca
     1321 cggacatgaa gaagcaggtt ctgaagttcg ggctgatctg cggggcgatt ctcggagcga
     1381 tgacggcggt catggtgccg ctgtgcatga acggcacggc gagcttcgac aacgccgaga
     1441 tcttcggcta cacgatccag gtgctcgcgt ttctcctcgt cttcttcgcc atccgctcgt
     1501 atcgcgagag cccgggaggc ggggcgatca cgttcgggaa ggccttccag gtcggaatcc
     1561 tcgtgacgct cgtcgcggcc gccgtctacg tcgtttcctg ggagatcgtc tattacaact
     1621 tcatccccga cttcggggac aagtacgccg cgcacatgct cgagaagatg aagacgaagg
     1681 gcgagagcgc gcaggcgatc gagaaggcga cgcgggagat ggcgaagttc aaggagctgt
     1741 accggaaccc gctcttcaac gccgggatga
//
LOCUS       DAOMFY010000485         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_37926_length_1743_cov_10.245853, whole genome shotgun
            sequence.
ACCESSION   DAOMFY010000485 DAOMFY010000000
VERSION     DAOMFY010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37926_length_1743_cov_10.245853"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1486)
                     /gene="pfp"
                     /locus_tag="PLB01_20260"
     CDS             complement(155..1486)
                     /gene="pfp"
                     /locus_tag="PLB01_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HQQ79687.1"
                     /translation="MSQQKNLAILVGGGPAPGINSVISSATIRAVLEGVDVIGVRDGF
                     EWIMKGDIEHVAPLGIDEVSRIHFRGGSHIGISRANPTKDAQHLENTVISLLRLNVSR
                     LVTIGGDDTAFSAMKLVEKADGRIKVVHVPKTIDNDLDLPAHVDTFGYQTARHYGVEI
                     VKNLMVDARTTSRWYFIIAMGRKAGHLALGIGKAAGATLTLIPEEFLGHSIRFKAIVD
                     TLVGAIVKRLSYGRRDGVAVIAEGLVEGLDPSELTGLEDVERDTHGNVRIAEVNIGEI
                     LKAAVQQRLKAFGIKTTIAAKNIGYELRCADPIPFDLEYTRDLGYCAAKYLLSGGNAA
                     MISMQGGHFVPIPFAELVDAATGRTKVRMVDIHSTRYAIARRYMLRLRRDDFEDPHEL
                     AKFAATAGLSLSDFRKEFEYLIEWELPALALGPEGEAAPERHGIVPKLDLP"
     gene            complement(1557..1631)
                     /locus_tag="PLB01_20265"
     tRNA            complement(1557..1631)
                     /locus_tag="PLB01_20265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1597..1599),aa:Gly,seq:gcc)
BASE COUNT          263 a          582 c          595 g          303 t
ORIGIN      
        1 ctcgggcgcc tgctcggcga cgcggacctg cgcgcacgtc tcggccgggc ggcccgcgcg
       61 acgttcgagg cccggttctc ggcctgcgcc ctctccggca cactggatgc cgtctaccgc
      121 gagctcgggg cctgacgggg tgcggctcgg gccgtcaagg caggtcgagc ttcgggacga
      181 tgccgtggcg ctccggcgcc gcctcgccct ccgggccgag cgcgagtgcg gggagctccc
      241 actcgatgag gtactcgaac tccttccgga agtccgagag cgagagcccc gccgtcgccg
      301 cgaacttcgc gagctcgtgg gggtcctcga aatcgtcgcg cctcaggcgc agcatgtagc
      361 gccgcgcgat cgcgtagcgc gtcgagtgga tgtccaccat ccggaccttc gtgcggcccg
      421 tggcggcgtc gacgagctct gcgaacggga tcggcacgaa gtggccgccc tgcatcgaga
      481 tcatcgcggc attcccgccc gagaggaggt acttcgcggc gcagtagccg aggtcgcgcg
      541 tgtactcgag gtcgaacggg atcgggtccg cgcacctgag ctcgtacccg atgttcttcg
      601 ccgcgatcgt ggtcttgatc ccgaacgcct tcagccgctg ctggacggcg gccttcagga
      661 tctcgccgat gttcacctcg gcgatccgga cgttcccgtg cgtgtcgcgc tcgacgtcct
      721 cgagccccgt gagctcggac gggtcgaggc cctcgacgag gccttccgcg atcacggcga
      781 cgccgtcgcg gcgcccgtac gagaggcgct tcacgatcgc gccgacgagc gtgtccacga
      841 tcgccttgaa gcggatcgaa tgcccgagga actcctcggg gatgagcgtg agggtcgccc
      901 cggccgcctt cccgatcccg agcgcgaggt ggcccgcctt gcgtcccatc gcgatgatga
      961 agtaccagcg cgacgtcgtt cgcgcgtcca ccatgaggtt cttgacgatc tcgacgccgt
     1021 agtggcgagc ggtctggtag ccgaacgtgt ccacgtgcgc ggggaggtcg aggtcgttgt
     1081 cgatcgtctt cgggacgtgg acgaccttga tgcgcccgtc cgccttctcg acgagcttca
     1141 tcgccgaaaa cgccgtgtcg tcgccgccga tcgtgacgag gcgcgagacg ttcaggcgca
     1201 ggagcgagat cacggtgttc tcgaggtgct gggcgtcctt cgtcgggttc gcgcgcgaga
     1261 ttccgatgtg cgagccgccg cggaagtgga tgcggctcac ctcgtcgatc ccgaggggag
     1321 cgacgtgctc gatgtctccc ttcatgatcc actcgaaccc gtcgcggacg ccgatgacgt
     1381 ccacaccctc gaggacggcg cgaatcgtgg ccgagctgat gacgctgttg atcccgggcg
     1441 cgggtccgcc cccgacgaga atggcgaggt tcttctgctg gctcacggcg aagccccgca
     1501 ctgatcttag accgcgaaga aaaaggcccg gctttcgccg ggccgatctc ttgaattgga
     1561 gcgggacacg agggtcgaac tcgcgacatc taccttggca aggtagcgct ctaccactga
     1621 gctagtcccg ctcaggagca ccggaagata agcgacgggg ctccggctgt caaggcgagc
     1681 gcttcggcgt gtcgtagctc catgaaagtg tcccctcgat aactcgatag aaatgtcccc
     1741 ttg
//
LOCUS       DAOMFY010000486         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_38131_length_1738_cov_6.366013, whole genome shotgun sequence.
ACCESSION   DAOMFY010000486 DAOMFY010000000
VERSION     DAOMFY010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38131_length_1738_cov_6.366013"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLB01_20270"
     CDS             <1..436
                     /locus_tag="PLB01_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="F420-dependent NADP oxidoreductase"
                     /protein_id="HQQ79688.1"
                     /translation="AVPHEAAGAVLKAAGPLAGKVLVDATNPLADRAQRLALPNTTSG
                     AEEIAKAAPGARVVKAFNTIGFNVMANPGFPGGAASLLFCGDDTDAKKSVASLAADLG
                     FDPVDAGPLSEARLLEPFALLWIHLAMFRGHGREIAFRLMKR"
     gene            462..836
                     /locus_tag="PLB01_20275"
     CDS             462..836
                     /locus_tag="PLB01_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79689.1"
                     /translation="MSLKDPNVFDPKSRKRVAIVISNPAVSTSTGWPVGFWWSELTHP
                     YYEFTEAGYEVEVFSPEGGACEPDAMSDPRDPSGYSAHDLVTLGFVHKPELLALTKNT
                     RKAADIDVGKSGPWASSPRTST"
     gene            806..1000
                     /locus_tag="PLB01_20280"
     CDS             806..1000
                     /locus_tag="PLB01_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79690.1"
                     /translation="MGLLPKDKHVMPWRIEDEMKKLGANYVQAGMWRGFAVRDGNLVT
                     GQQNFSGSETARLVIETLGR"
     gene            1199..1273
                     /locus_tag="PLB01_20285"
     tRNA            1199..1273
                     /locus_tag="PLB01_20285"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Val,seq:gac)
     gene            1314..1389
                     /locus_tag="PLB01_20290"
     tRNA            1314..1389
                     /locus_tag="PLB01_20290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1347..1349,aa:Val,seq:cac)
     gene            1469..>1738
                     /locus_tag="PLB01_20295"
     CDS             1469..>1738
                     /locus_tag="PLB01_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HQQ79691.1"
                     /translation="MSSVSAMRDGVSAEGGVIKLTLPDGSVREVPSGTTGRAVAESIG
                     PRLAKDALGVKLDGKVLDLSRPLTESGKFEVVTPKHPDALELYRHS"
BASE COUNT          297 a          588 c          576 g          277 t
ORIGIN      
        1 cgccgtcccg cacgaggcgg ccggcgcggt cctgaaggcg gccgggcctc tcgccggcaa
       61 ggtcctcgtg gacgcgacga atccgcttgc ggaccgcgcc cagcgcctcg cgctcccgaa
      121 cacgacgtcc ggcgccgagg agatcgcgaa ggcggcgccc ggtgcaaggg tcgtcaaggc
      181 cttcaacacg atcggcttca acgtcatggc gaatccgggc ttcccggggg gcgccgcgtc
      241 gctcctcttc tgcggcgacg ataccgacgc gaagaagtcg gttgcctccc tcgcggccga
      301 cctcggcttc gatcccgtgg acgcgggccc gctttccgaa gcccgtctcc tcgagccctt
      361 cgccctcctc tggatccacc tcgcgatgtt ccgcggccac ggccgggaga tcgcgttccg
      421 cctcatgaag cgctgaccga cgccaggacc aaggagaacc cgtgagcctc aaggacccga
      481 acgtcttcga cccgaaatcg aggaagcgcg tcgcgatcgt gatctcgaac cccgccgtgt
      541 cgacgtcgac cggctggccc gtcgggttct ggtggagcga gctgacgcac ccgtactacg
      601 agttcaccga ggccggctac gaggtcgagg tgttcagccc cgagggcgga gcctgcgaac
      661 cggacgcgat gagcgatccc cgcgacccga gcggctactc ggcgcacgac ctcgtgacgc
      721 tcggcttcgt gcacaagccc gagctcctcg ccctgacgaa gaacacccgg aaggccgccg
      781 acatcgacgt cgggaagtct ggtccatggg cctcctcccc aaggacaagc acgtgatgcc
      841 gtggcgcatc gaggacgaga tgaagaagct gggcgcgaac tacgtgcagg ccgggatgtg
      901 gcgcgggttc gccgtccgcg acggcaacct cgtgacgggt cagcagaact tctcggggtc
      961 cgagacggcc cggctcgtga tcgagaccct cggccggtga gcaagtcccg cctcgaggcg
     1021 ttcagcgacg gtctttcgcg aggagccgcc gggggctgtg ttatcctccg cggctccgga
     1081 agaaacgccg gaaatgctct ttggagaaga cgatccgggt cgaggtcggg gctcaaaaga
     1141 cgttccatcg cgaacggccc ccggcgacag ccctcggaga gacaaacgcg gcacgcgcag
     1201 gcgcttagct cagtggtaga gcactacctt gacacggtag gggtcagcag ttcgatcctg
     1261 ctagcgccta ccatccgcgg tctctcccgt gccgccgccg tttggcagct cgaaggcgct
     1321 tagctcagtc ggtagagcac ttccttcaca cggaaggggt cagcggttcg agtccgctag
     1381 cgcctaccat tccctccctc gaactgaaat cgtttttctt ctcggagaga agagaagaag
     1441 aatttcttag aagggaagag ggtcgggtat gtcctcggtg agcgcgatgc gcgacggtgt
     1501 ttcggcggag gggggcgtca tcaagttgac gctgcccgac ggctcggttc gggaggtgcc
     1561 ttcgggcacc acgggccgtg ccgtggccga gtccatcggt ccccgccttg ccaaggacgc
     1621 gctcggcgtc aagctggacg gaaaggtcct cgacctctcg cgcccgctca cggagtccgg
     1681 gaagttcgag gtcgtgacgc cgaagcaccc cgacgccctc gagctctacc gccactcc
//
LOCUS       DAOMFY010000487         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_38326_length_1733_cov_5.231824, whole genome shotgun sequence.
ACCESSION   DAOMFY010000487 DAOMFY010000000
VERSION     DAOMFY010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38326_length_1733_cov_5.231824"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLB01_20300"
     CDS             complement(<1..280)
                     /locus_tag="PLB01_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79692.1"
                     /translation="MPSTGGRRWIVPRRDLAAGGATLVRLSHPLLPEILQARPASEGL
                     RLTLAGPEPAPMVRLASAGRRAFLARLAALLGFLRFHGLGLAPEDLAAL"
     gene            387..1541
                     /locus_tag="PLB01_20305"
     CDS             387..1541
                     /locus_tag="PLB01_20305"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HQQ79693.1"
                     /translation="MTAPATPRRVLLLGVVWLAFVWAVALVAARSTPWLTARTGWSEE
                     LSRRLTPLARWDSGWYVSIAEGGYEAPPVRVGQETNHAFFPLYPALMRGAARATGLDV
                     ARAGALVSAAALLGALLLLAAWTRRGFGEERVWPTLLAFLAFPTSFFFAAVYSESLLL
                     FLALAAVLAAEKDRDGLAAAAGLLSGLTRISGLVLAPALFLVSWRRSRELGRPAPRAL
                     LRAGLVGASPLAGFGLFCLYFHERFGDALLFVKAQHNWSREAKTVFDGPRLILEEVWR
                     DLSTGHILAKSPVRTMEGVFLLLFLFLAWRLAARRLWPEALYVFLTVAIVFTSGTLES
                     GGRYVLPAFPAFAVLGGLSSRPAFFRALVALSIVAQAGYVWLYVHWLWVG"
     gene            complement(1545..>1733)
                     /locus_tag="PLB01_20310"
     CDS             complement(1545..>1733)
                     /locus_tag="PLB01_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006007819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79694.1"
                     /translation="SIAGRSGAPRAASFAAHGDHRVAMSAAVLALALPAGSMLDDPAC
                     VGKSWPGFWDAWNPLARS"
BASE COUNT          189 a          650 c          610 g          284 t
ORIGIN      
        1 caagggcggc gaggtcctcc ggcgcgaggc cgaggccgtg gaacctgaga aagccgagga
       61 gcgcggcgag tcgcgcgagg aaggcgcggc gcccggccga cgcgaggcgg accatcggcg
      121 ccggctcggg gccggcgagc gtgagtcgaa gcccctcgga tgcgggccgc gcctgcagga
      181 tctccggcag gagggggtgc gaaagccgca cgagcgtcgc gccgcccgcc gcgaggtcgc
      241 ggcggggaac gatccaacgt ctccctcctg tcgaaggcac gtcgcgtcct ccccgtggac
      301 gggtggatcg aagctgtcgc aggcccgacg atttcgaatt ccgggcctcg cgtcaagggc
      361 cgcgccgcct atcatcggga gaccccatga ctgcgcccgc caccccgcgc cgcgtcctcc
      421 tcctcggggt cgtctggctg gccttcgtgt gggcggtggc cctcgtcgcc gcgcgatcca
      481 cgccgtggct gaccgcccgg accggctggt cggaggagct ctcgcggcgc ctgacgcctc
      541 tcgcgcggtg ggactcgggc tggtacgtct ccatcgccga aggaggctac gaggcgccgc
      601 ccgtgcgcgt cggccaggag acgaatcacg ccttcttccc gctctacccc gccctcatgc
      661 gcggcgccgc gcgggcgacg ggtctggacg tcgcgcgcgc gggcgcgctc gtctccgcgg
      721 ccgcgctcct cggggcgctc cttctcctcg cggcgtggac gcgaaggggt ttcggagagg
      781 agcgcgtgtg gcccacgctt ctcgccttcc tcgcgtttcc gacgtcgttc ttcttcgcgg
      841 ccgtctactc cgagtcgctg ctgctgttcc tggctctcgc cgcggttctc gccgccgaaa
      901 aagaccggga tggccttgcc gcggccgcgg gcctcctctc gggcctcacc cggatctctg
      961 gcctcgtcct ggcgccggcg ctcttcctcg tctcgtggcg gaggagccgc gagctcgggc
     1021 gacccgcccc tcgggcgctg cttcgggccg ggctcgtcgg cgcatcgcca ctggcgggtt
     1081 tcggcctctt ctgcctgtac ttccacgaac gcttcggcga cgcgctcctc ttcgtgaagg
     1141 cgcagcacaa ctggtcgcgc gaggcgaaga ccgtcttcga cggcccgcgc ctcatcctcg
     1201 aggaagtctg gcgcgacctt tcgaccggcc acatcctcgc gaagagcccc gtccgcacga
     1261 tggagggcgt cttcctcctc ctcttcctct ttctcgcatg gcggctcgcg gcccggcggc
     1321 tctggcccga ggcgctctac gtcttcctga ccgtcgcgat cgtgttcacg tccgggactc
     1381 tcgagagcgg cgggcgctac gttctgcccg cgttccccgc gttcgcggtc ctcggcggcc
     1441 tctcgtcacg ccccgcgttc ttccgcgctc tcgtcgcgct ctctatcgtc gcgcaggcgg
     1501 gctacgtctg gctctacgtc cactggctct gggtcggctg aacgtcagga acgggcgagc
     1561 gggttccagg cgtcccagaa gccgggccac gacttgccga cgcacgcggg gtcgtcgagc
     1621 atcgagccgg cgggcagcgc gagagcgagg acggccgccg acatcgcgac gcggtgatct
     1681 ccgtgcgcgg cgaacgaggc cgcgcgcggc gcgccgctcc ggcccgcgat cga
//
LOCUS       DAOMFY010000488         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_38595_length_1726_cov_6.981448, whole genome shotgun sequence.
ACCESSION   DAOMFY010000488 DAOMFY010000000
VERSION     DAOMFY010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38595_length_1726_cov_6.981448"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1042
                     /gene="mazG"
                     /locus_tag="PLB01_20315"
     CDS             206..1042
                     /gene="mazG"
                     /locus_tag="PLB01_20315"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQQ79695.1"
                     /translation="MQKNLPVGDPGDAAAALVRLMERLRRECPWDRKQTFQTLSGYLL
                     EECHEVLDALQREDFGELEGELGDLLFEIVFLAELGRERGDFDFTSLARRIHDKMVAR
                     HPHVFGEASVRDAEGVRVQWEELKRKEREKDGRPPSPFDGVPRALPALLKAVRLTSRA
                     ADLGFDWERDADVLAKLDEEVAEFKAELSAPADADAAVHKDRVTGEIGDLLFTVVNVA
                     RRHGVDPEAALQGTNDKFRRRFEEVARRARAAGKEIKGTPLTELDALWDQVKSEEHEK
                     IS"
     gene            complement(1153..>1726)
                     /locus_tag="PLB01_20320"
     CDS             complement(1153..>1726)
                     /locus_tag="PLB01_20320"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HQQ79696.1"
                     /translation="APFATRAVALAKEKGWDLLVHLPMEPEAGPSEAEAIGARDTDDV
                     IRAKLLQALARTPGALGVNNHQGSKATTDARVVRAVLGVVKEKGLFFLDSRTTGASVV
                     GTEAAAMHVPFLARDVFLDDVAAETSAKGGAPEALDAAWERTLQVAAKKGEAIVIGHP
                     HKETLAFLEKRLASERSAGARLVKVSELVP"
BASE COUNT          259 a          623 c          585 g          259 t
ORIGIN      
        1 gcggccggct ccgtgggccc ggaggcgggg gccgccgcgg gcacggcggg ctcgggcgcc
       61 gacgcctcgg ccggcacgtc gtcgcgaacc tcgccctccg cggtgaagag gcggcggtcc
      121 acgaccttga tggtcttcgg gttttcggac atagaacccc tcgggtcgtc gtattttacg
      181 ggacgcgagc gggaggtcag cctcgatgca gaaaaatctt ccagtcgggg accccggcga
      241 cgccgcggcg gcgctcgtcc gcctcatgga aaggctccgg cgggagtgtc cctgggaccg
      301 gaaacagacg ttccagaccc tgtcgggcta cctcctcgag gagtgccacg aggtcctcga
      361 cgcgctgcag cgcgaggatt tcggcgaact ggagggcgag ctcggcgacc ttctcttcga
      421 gatcgtcttc ctcgccgagc tcggccggga gcgcggcgac ttcgacttca cgtcgctcgc
      481 ccgccgcatc cacgacaaga tggtcgcgcg ccacccccac gtcttcggcg aggcgagcgt
      541 ccgcgacgcc gagggcgtgc gcgtccagtg ggaggagctg aagcgcaagg agcgcgagaa
      601 ggacggccgg ccgccctccc ccttcgacgg agtgccccgt gcgctcccgg cactcctcaa
      661 ggcggtgcgc ctcacgtcgc gcgccgccga cctcggcttc gactgggagc gggacgccga
      721 cgtcctcgcg aagctggacg aggaggtcgc cgagttcaag gccgagctct cggcgcccgc
      781 ggatgcggat gcggccgtcc acaaggaccg cgtcacggga gagatcggcg acctcctctt
      841 caccgtcgtc aacgtcgctc gccgtcacgg cgtcgacccc gaggccgcgc tgcagggcac
      901 gaacgacaag ttccggcggc ggttcgagga ggtggcgcgg cgcgcacgcg ctgcagggaa
      961 ggaaatcaag ggcacgccgc tgaccgaact cgacgctctc tgggatcagg tcaagtccga
     1021 agagcacgag aagatttctt agaaggttaa gaggggaaac cgcgcgcaag gggctgccca
     1081 tggcggcgac gccgcggcgc gcccgattca ccttctaaga agaattctct cttcctcctg
     1141 ctcttcctct tcctacggaa ccagctcgga gaccttgacg agcctcgccc cggcgctcct
     1201 ctccgaagcc aatctcttct ccagaaaagc cagcgtctcc ttgtgcgggt ggccgatcac
     1261 gatggcctcg cccttcttcg ccgcgacctg gagcgtacgc tcccacgccg cgtccagcgc
     1321 ctccggggcg ccgcccttcg cgctcgtctc cgccgcgacg tcgtcgagga acacgtcccg
     1381 cgcgaggaac ggcacgtgca tcgccgccgc ctccgtcccg acgaccgagg cacctgtcgt
     1441 ccgcgagtcg aggaagaaga gccccttttc cttcacgacg ccgaggaccg cgcgcacgac
     1501 gcgcgcgtcc gtcgtcgcct tcgagccctg gtggttgttg accccgaggg cgccgggtgt
     1561 ccgcgcgagc gcctgaagca gcttcgcgcg gatcacgtcg tccgtgtcgc gcgcgccgat
     1621 cgcctccgct tcggacgggc ccgcctcggg ctccatcggg aggtggacga ggaggtccca
     1681 ccccttctcc ttcgcgagcg ccaccgcacg cgtggcgaac ggcgcg
//
LOCUS       DAOMFY010000489         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_38978_length_1717_cov_6.903129, whole genome shotgun sequence.
ACCESSION   DAOMFY010000489 DAOMFY010000000
VERSION     DAOMFY010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38978_length_1717_cov_6.903129"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..962)
                     /locus_tag="PLB01_20325"
     CDS             complement(174..962)
                     /locus_tag="PLB01_20325"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ79697.1"
                     /translation="MIEDRDPWWEHWFGEPYLALYPERDDREARTQAVFAREVLSPFA
                     KAGRISFLDLGCGTGRRAAALGSGLGATVGVDASFRLVGSVRRLGPRLRPGALCAIPE
                     SLPLASRCVGAAVSFASAFGRSAEPADDARVAGEIARVLAPGGGFLAAVVNAERVVAG
                     LARREEKLIQGTRVVIRRRYDPERRMLEKEIELGTGPDRRIYAQRARALTEHELRGRL
                     RTAGLTVVGAWGDFDGSAFERGRSPRLVLLASKPSSKAFGAAGR"
     gene            complement(1010..1414)
                     /gene="atpC"
                     /locus_tag="PLB01_20330"
     CDS             complement(1010..1414)
                     /gene="atpC"
                     /locus_tag="PLB01_20330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HQQ79698.1"
                     /translation="MDGRLTLTVVTPERAVVAGVLCDEVTLPGLEGEMGILPAHTPLI
                     ALLGIGLVTFRDGSKKTSVAVRGGFAEIAGDAVRVLADEAAAKDAIDAGKAAQEKTAA
                     EARRADVVGDEQLDAANADARFADARIAVAAS"
     gene            complement(1419..>1717)
                     /locus_tag="PLB01_20335"
     CDS             complement(1419..>1717)
                     /locus_tag="PLB01_20335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525129.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQQ79699.1"
                     /translation="KYKDLQDIIAILGIDELSEDDKVIVARARKIQRFLSQPFHVAEQ
                     FTGLPGRYVKVADTIRSFKEIVEGKHDELPEQAFFNVGAIEEAQEKAEKLRGAA"
BASE COUNT          228 a          637 c          619 g          233 t
ORIGIN      
        1 gtccgcgccc gccgccgtgg ggcgaggacg gaacgcgcgg cggaccgtcg cggcctgcgc
       61 ggcgatcgcg gccagcgtcg ccgtgcgcgg caggaactcc gacacgtccg gcgtgcccgt
      121 ggcgagcccg cgagcgagcg ccggaatgcc cgggacgcgg tcgaggggaa tcgtcatcgg
      181 cccgcggctc cgaacgcctt cgacgagggc ttcgacgcga ggagcacgag gcggggcgag
      241 cgcccgcgct cgaacgccga gccgtcgaag tccccccacg ctcccacgac cgtcagcccc
      301 gcggtgcgca ggcggccgcg caactcgtgc tcggtgaggg cgcgggctcg ctgcgcgtaa
      361 atgcgtctgt cggggccggt ccccagctcg atctcctttt ccagcatgcg ccgctcggga
      421 tcgtaccgtc gccggatcac gacgcgcgtt ccctggatca gcttttcctc gcgccgcgcg
      481 aggcccgcga cgacgcgctc ggcgttcacg accgccgcga ggaacccgcc tcccggcgcg
      541 aggacacggg cgatctcgcc ggcgacgcgc gcgtcgtcgg ccggctcggc cgagcggccg
      601 aacgcgctcg cgaagctcac ggccgcgccg acgcaccggc tcgcgagggg caggctctcc
      661 gggatcgcgc agagggcgcc gggcctgagg cgcgggccga ggcgccgcac ggatccgacg
      721 aggcggaagg aggcgtcgac gcccacggtc gcaccgagtc cgctcccgag ggccgcggcc
      781 cggcggcccg ttccacagcc gagatcgaga aacgagatgc ggcccgcctt cgcgaagggg
      841 gagaggacct cgcgtgcgaa caccgcctgg gtccgcgcct cgcgatcgtc acgctccgga
      901 tagagcgcga gatacggctc tccgaaccag tgctcccacc agggatcgcg gtcctcgatc
      961 acgccgccac cggcgccgat gataggcgga actcccgggc cgggcccgat caggacgccg
     1021 cgaccgcgat ccgcgcgtcg gcgaaccggg cgtcggcgtt ggcggcgtcg agctgctcgt
     1081 cgcccacgac gtccgcgcgg cgcgcctcgg ccgccgtctt ctcctgcgcg gctttcccgg
     1141 cgtcgatcgc gtccttcgcg gccgcttcgt cggcgaggac gcgcacggcg tcgcccgcga
     1201 tctcggcgaa cccgccccgc acggcgacgg acgtcttctt cgatccgtcg cggaaggtca
     1261 cgaggccgat cccgaggagc gcgatgagcg gcgtgtgcgc ggggagaatc cccatctcgc
     1321 cctcgagtcc cggcagcgtc acctcgtcgc agaggacgcc cgcgacgacg gcgcgttccg
     1381 gggtcacgac ggtcagcgtg agacgtccgt ccacggcgtc aggccgctcc ccggagcttt
     1441 tcggccttct cctgtgcttc ctcgatcgcg ccgacgttga agaacgcctg ctccggcagc
     1501 tcgtcgtgct tgccctcgac gatttccttg aaggagcgga tcgtgtcggc gaccttcacg
     1561 taccggcccg gcaggcccgt gaactgctcg gcgacgtgga acggctgcga gaggaagcgc
     1621 tggatcttcc gggcgcgggc cacgatgacc ttgtcgtcct cggagagctc gtcgatgccg
     1681 aggatcgcga tgatgtcctg gaggtccttg tacttct
//
LOCUS       DAOMFY010000490         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_39460_length_1706_cov_5.631133, whole genome shotgun sequence.
ACCESSION   DAOMFY010000490 DAOMFY010000000
VERSION     DAOMFY010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39460_length_1706_cov_5.631133"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1706
                     /locus_tag="PLB01_20340"
     CDS             33..>1706
                     /locus_tag="PLB01_20340"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HQQ79700.1"
                     /translation="MRTLPISARTRRWLAWIAAFVAVVAVLGFLVAPPIVRSQAEKVL
                     ADSLHRPVSIENVRVNPFAPSVTVAGLAVKERDGSTVVAGFDELYVRLSYTSIFRLAP
                     VVSAVRLEKPVLRVVRRPDRTYNFQDLVDELLARKPAEGREPVRFSVNNIQVLDGRIT
                     FDDAVAGEKHEVAGLRVGIPFVSSLPSQVDIDVEPELSATVDGAPVAIRGDTHPFKDT
                     NETNVEVNLDALPLPKLADYSPVPLRFRLDSGLLDTRIRIRFVTRDAKPVGLAASGGI
                     GISKLAIADRAGSPLVAFERLAVDVETIDVFGREARVRSVRLDRPVANVVRSKEGGLN
                     LVEALPLPSPATATDATPPTPEKPFAFAVEDVTVAGGKASLVDLSVTPRAFRTELEAI
                     DVGVKGLSSRPDSRAQVTARFTTDGLGAFRHEGTLGLSPVVAEGRVAGEGFRLARIYP
                     YLEPVLNVDIAEGSLDFEAGYALAFAGDSPELRVSGASATLRSVALRYPGEKEAFLRL
                     PLAEASDASADLAMRRVRLGQVIAKGGALAAHREADGTLRFSRLIRRQSE"
BASE COUNT          225 a          605 c          613 g          263 t
ORIGIN      
        1 ccatgagcgc accgccccgc ttgctcgagc gagtgcgtac gctgccgata tcggcgcgca
       61 cgcgccggtg gctggcctgg atcgcggcct tcgtggccgt cgtggccgtg ctgggctttc
      121 tcgtggcgcc gccgatcgtg cgctcgcagg ccgagaaggt cctcgccgat tccctgcacc
      181 ggcccgtctc gatcgagaac gttcgcgtca accccttcgc cccctccgtc acggtcgcgg
      241 gactcgcggt caaggaacga gacggttcca ccgtcgtcgc cgggttcgac gagctctacg
      301 tgcgactctc gtacacgtcg atcttccgcc tggcgcccgt cgtgagcgcc gtgcggctgg
      361 agaagccggt cctccgggtg gtccgccggc ccgaccgcac ctacaacttc caggacctcg
      421 tcgacgagct gctggcccgc aagccggcgg aggggcggga gccggtccga ttctcggtca
      481 acaacatcca ggtgctggac gggcgcatca cgttcgacga cgcggtcgcc ggcgagaagc
      541 acgaggtcgc gggactgcgc gtggggatcc cgttcgtctc gagcctgccc tcgcaggtcg
      601 acatcgacgt cgagcctgaa ctctcggcga cggtcgatgg cgcccccgtg gcgatccggg
      661 gcgacacgca cccgttcaag gacacgaacg aaacgaacgt cgaggtgaac ctcgacgcgc
      721 ttccccttcc gaaactcgcg gactactcgc cggtgccgct tcgcttccgc ctggactccg
      781 ggctgctcga cacgcgcatc cggatccggt tcgtgacgcg cgacgcgaag ccggtcggcc
      841 tcgccgcgtc gggcgggatc gggatctcga agctggcgat cgccgaccgg gccggaagcc
      901 cgctcgtggc cttcgagcgg ctggcggtcg atgtcgagac catcgacgtc ttcggccggg
      961 aggcgcgcgt gcgatcggtc cgcctcgacc ggcccgtcgc gaacgtcgtg cgctcgaagg
     1021 aaggcgggct caacctggtc gaggcgctgc cgcttccctc gccggccacg gccacggatg
     1081 cgacgcctcc gacgcccgag aagccgttcg ccttcgcggt ggaggacgtc acggtcgcgg
     1141 gcggcaaggc ctcgcttgtg gacctgtcgg tcacgccgcg cgcgttcagg acggagctcg
     1201 aggcgatcga cgtgggcgtg aaggggctct ccagccggcc ggatagccgg gcgcaggtga
     1261 cggcacgctt cacgacggac ggcctcggcg cgttccgaca cgagggcacc ctcgggcttt
     1321 cgcccgtcgt ggccgagggg agggtggccg gcgagggatt ccggctggcg cgcatctacc
     1381 cctatctcga gccggtgctc aacgtcgaca tcgcggaagg ctcgctcgac ttcgaggcgg
     1441 gctacgcgct cgcgttcgcc ggcgactcgc cggagctgcg ggtgtcgggg gcgagcgcca
     1501 cgttgcggtc ggtcgcgctg cgctatcccg gcgagaagga agccttcctc cggctgccgc
     1561 tcgccgaggc aagtgacgct tcggcggacc tcgcgatgcg gcgggtgcgc ctgggccagg
     1621 tgatcgccaa gggcggcgcg ctggccgccc accgcgaggc cgacggcacg ctccggtttt
     1681 cgcggctcat ccgccggcag tcggaa
//
LOCUS       DAOMFY010000491         1691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_40085_length_1691_cov_5.147922, whole genome shotgun sequence.
ACCESSION   DAOMFY010000491 DAOMFY010000000
VERSION     DAOMFY010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40085_length_1691_cov_5.147922"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /gene="trpD"
                     /locus_tag="PLB01_20345"
     CDS             complement(<1..539)
                     /gene="trpD"
                     /locus_tag="PLB01_20345"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQQ79701.1"
                     /translation="MTPVHHALREVARGGKLSREDARRAVASLLEDEIPPAVAGGFLA
                     ALAAAGETIEVLAGAVDVLRDRATRPPVTGFLAARAVDVCGTGGDDLGTFNVSTAAAF
                     VVAGAGTPVAKHGNRAVSSKCGSADVLAALGIVLEMTPERAALALDIANITFLFAPLY
                     HPVMKKLAPLRRELGVRTV"
     gene            complement(536..1105)
                     /locus_tag="PLB01_20350"
     CDS             complement(536..1105)
                     /locus_tag="PLB01_20350"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQQ79702.1"
                     /translation="MILVVDNYDSFTFNLVQILATLDPDVVVVRNDALSVDEILARRP
                     DRIVVSPGPGRPEDAGVSCALIERNTEVPLLGVCLGHQALGAVFGGRVSRAPVLMHGK
                     TSEIAHDGRGLFEGLSNPFTATRYHSLAVEADSVPDALRVTARTPDGIVMGLAHRTRP
                     LVGVQFHPESILTTEGEKLLKNFVAGRLS"
     gene            complement(1102..>1691)
                     /locus_tag="PLB01_20355"
     CDS             complement(1102..>1691)
                     /locus_tag="PLB01_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HQQ79703.1"
                     /translation="GGAAVETIPLAGTRPRGATREEDLALEKDLLADAKERAEHVMLV
                     DLGRNDLGRVSVPGTVRVKDFFAVERYSHVMHIASRVVGTLAPGRHAVDALAATFPAG
                     TLTGAPKIRAMEIIEDLEPVRRGAYGGAVGYLDFAGNLDVAIAIRTAVVANGMLHVQA
                     GAGIVADSVPESEALECENKARALMRAVARAGEFP"
BASE COUNT          242 a          593 c          633 g          223 t
ORIGIN      
        1 aagaccgtcc gcacgccgag ctcgcggcgc agcggagcga gtttcttcat gaccgggtgg
       61 tagagcggcg cgaagaggaa cgtgatgttc gcgatgtcga gagcgagcgc ggcgcgctcc
      121 ggcgtcatct cgaggacgat gccgagggcc gcgaggacgt ccgccgagcc gcacttcgac
      181 gagacggcgc ggttgccgtg cttggcgacg ggcgtccccg cgcccgcgac gacgaacgcg
      241 gccgcggtgg agacgttaaa cgtcccgagg tcgtcgccgc cggtcccgca gacgtccacg
      301 gcgcgggcgg cgaggaaccc cgtgacgggg ggccgcgtcg cgcggtcccg aaggacgtcg
      361 acggcccctg cgaggacctc gatcgtctcg ccggcggccg cgagcgcggc gaggaaacca
      421 cccgcgacgg ccggcggtat ctcgtcctcg aggagggagg ccacggcccg acgggcgtcc
      481 tcgcgcgaga gctttccccc gcgcgcgacc tcgcggaggg cgtggtgaac gggcgtcacg
      541 acagccgtcc cgccacgaag ttcttcagga gcttctcccc ctcggtcgtc aggatcgact
      601 ccgggtggaa ctggacgccc acgaggggac gcgtgcggtg cgcgaggccc atgacgatcc
      661 cgtctggcgt cctcgccgtc acgcgcagcg cgtcggggac ggagtccgcc tcgacggcga
      721 gcgagtggta gcgcgtcgcc gtgaatggat tcgagagccc ttcgaagagt ccgcgcccgt
      781 cgtgcgcgat ttcggacgtc ttcccgtgca tgagaacggg cgcccgcgac acgcggccgc
      841 cgaacacggc gccgagagcc tggtgtccga ggcagacgcc gagaagcggg acctccgtgt
      901 tgcgttcgat gagcgcgcag gagacgcccg cgtcctcggg ccggcccggc ccgggcgaca
      961 cgacgatgcg gtccggccgc ctcgcgagga tctcgtccac ggacagggcg tcgttccgca
     1021 cgacgaccac gtcggggtcc agcgtcgcga ggatctggac gaggttgaac gtgaacgagt
     1081 cgtagttgtc gacgacgagg atcacgggaa ctcccccgcc cgcgcgacgg cccgcatgag
     1141 ggcccgcgcc ttgttctcgc actcgagggc ttcggactcg gggacggaat cggccacgat
     1201 tcccgccccg gcctgcacgt ggagcatccc gttcgcgacg accgccgtgc ggatcgcgat
     1261 cgcgacgtcc agattgccgg cgaagtcgag gtacccgacg gctccgccgt atgcgccgcg
     1321 ccggacgggc tcgaggtcct cgatgatctc catggcccgg atcttcggcg cgcccgtgag
     1381 cgtgccggcc gggaacgtcg ccgcgagggc gtcgacggcg tggcggcccg gcgcgagcgt
     1441 cccgacgacg cgggaggcga tgtgcatgac gtgcgagtag cgctccacgg cgaagaagtc
     1501 cttcacgcgc acggtcccgg gcacggacac gcgcccgagg tcgttgcgtc cgaggtccac
     1561 gagcatgacg tgctcggccc gctccttggc gtcggcgaga aggtccttct cgagcgcgag
     1621 gtcctcctcg cgcgtcgcgc cgcgcggccg cgtgccggcc agcgggatcg tctcgacggc
     1681 cgcgccgccc g
//
LOCUS       DAOMFY010000492         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_40475_length_1682_cov_6.433313, whole genome shotgun sequence.
ACCESSION   DAOMFY010000492 DAOMFY010000000
VERSION     DAOMFY010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40475_length_1682_cov_6.433313"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..682)
                     /locus_tag="PLB01_20360"
     CDS             complement(98..682)
                     /locus_tag="PLB01_20360"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQQ79704.1"
                     /translation="MKRIASALVLTALVLTVSAATAQAPPAGFRGDFLAQQDGVEKEI
                     LGLAEATPADKFKWRPAEGVRSISEVYMHLAFGNYLLTGFVGAKTPDGITGGMEKTVT
                     EKAAVIDALKKSFAHLRAAVAATPDADLGKAMKFFGRDSTVGGILLAAANHEHEHLGQ
                     SIAYARMNGIVPPWTAERQAKAAAKPAEKPAEKK"
     gene            779..1630
                     /locus_tag="PLB01_20365"
     CDS             779..1630
                     /locus_tag="PLB01_20365"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQQ79705.1"
                     /translation="MTKIRFSAAALALAAAPALAQLATPMPSPNASVTQTFGVTKAEV
                     VYSRPSVKGRTIWGDLVPYDKVWRTGANAVTKISFDTDVTVEGQKLPAGAYGLFTIPG
                     KAEWTVIFNKQSTGSPADYAADKDVLRVKVKPAALAENKEFFTISFPAASNAGATLVL
                     AWEKLKISLAIGVDTNAAFLHKAKVAVADAKVDDWRTSYMAARGLYDMKYALEDAAKF
                     LERSLSTKETFSNLSLKATVLAGKGDKKGAIAAAEKALAAGKAAEQKPSPEAVAELEK
                     SIAGWKQ"
BASE COUNT          273 a          560 c          600 g          249 t
ORIGIN      
        1 gaggccgacg ggcagcgcaa agatgaggtc ccgcgacgcg aggacggcgg cgtccgggag
       61 aagcggaacc ggcgagaaga gcgtcaaccc gcgcgcgcta tttcttctcg gcgggctttt
      121 cggcgggctt cgccgcggcc ttcgcctgcc gctcggcggt ccacggcggg acgatgccgt
      181 tcatccgggc gtaggcgatc gactggccga ggtgctcgtg ctcgtggttc gccgcggcga
      241 gcaggatccc gccgacggtc gagtcgcggc cgaagaactt catggccttg ccgaggtcgg
      301 catccggcgt cgcggcgacg gccgctcgca ggtgcgcgaa cgacttcttg agcgcgtcga
      361 tcacggcggc cttttccgtc acggtcttct ccatgccgcc ggtgatgccg tcgggcgtct
      421 tggcgccgac gaagcccgtc aggaggtagt tcccgaaggc gaggtgcatg taaacctcgc
      481 tgatcgagcg gactccctcg gccggacgcc acttgaactt gtccgcgggg gtggcctcgg
      541 ccaggccgag gatttccttt tcgacgccgt cctgctgggc gaggaagtcg ccgcggaagc
      601 ccgccggcgg cgcctgcgcc gtggcggcgg agacggtgag gacgagggcc gtgaggacga
      661 gggccgacgc gattctcttc atggcggggt ctcctttgag acgggatctt atccatccgc
      721 gtagaattcg cggattcgag gcgttccgcc cgaaaaacga accgtggagg actcaccgat
      781 gacgaagatc cgcttctccg ccgccgccct cgccctggcg gccgctccgg cgctcgcgca
      841 gctcgcgacg ccaatgccga gcccgaacgc gtccgtcacc cagacgttcg gcgtcacgaa
      901 agccgaggtc gtctacagcc gcccgtccgt caagggccgc acgatctggg gcgacctcgt
      961 tccgtacgac aaggtgtggc gcaccggcgc gaacgcggtc acgaagatct cgttcgacac
     1021 ggacgtgacg gtcgagggcc agaagctgcc ggccggcgcc tacggcctct tcacgatccc
     1081 gggcaaggcc gaatggaccg tcatcttcaa caagcagtcc acgggctcgc cggccgacta
     1141 cgcggccgac aaggacgtcc tgcgcgtcaa ggtgaagccg gcggcgctgg ccgagaacaa
     1201 ggagttcttc acgatctcgt tcccggccgc gtccaacgcc ggcgcgacgc tcgtgctcgc
     1261 gtgggagaag ctgaagatct cgctcgcgat cggcgtcgac acgaacgccg ccttcctcca
     1321 caaggcgaag gtggcggtcg cggatgcgaa ggtcgacgac tggcgcacgt cgtacatggc
     1381 cgcgcgaggc ctctacgaca tgaagtacgc cctggaggac gcggcgaagt tcctcgagcg
     1441 gtcgctctcc acgaaggaga cgttctcgaa cctgtcgctg aaggcgaccg ttctcgcggg
     1501 caagggcgac aagaagggtg cgatcgcggc ggccgagaag gcgctcgccg ccggcaaggc
     1561 cgccgagcag aagccctcgc ccgaggcggt cgccgagctc gagaagtcga tcgcgggctg
     1621 gaagcagtaa ggggctagcg cgacgcgggc gctcccggcg cccgcgtcgc gaggtcggcg
     1681 cc
//
LOCUS       DAOMFY010000493         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_41759_length_1653_cov_6.468711, whole genome shotgun sequence.
ACCESSION   DAOMFY010000493 DAOMFY010000000
VERSION     DAOMFY010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41759_length_1653_cov_6.468711"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1653
                     /locus_tag="PLB01_20370"
     CDS             <1..>1653
                     /locus_tag="PLB01_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQQ79706.1"
                     /translation="FFNPERYDLSKVGRLKLNYKFGLEEPLDNQVLTKRDVLEVVRFL
                     IDLKNGSKERDIDDIDHLGNRRVRAVGELLENQYRIGLVRMERAIKERMSLQEIETLM
                     PHDLINAKPVTAVIKEFFGSSQLSQFMDQTNPLSEVTHKRRLSALGPGGLSRERAGFE
                     VRDVHATHYGRICPIETPEGPNIGLISSLSCFARISEFGFIESPYKKVRNGRVLDHYE
                     IVTVGDSGWKKGQIVEAEELEEAVLRLKKGHKRQPVGRPTAFFQPAWEEENYTIAQAN
                     AEVDDKGRFTEDRIVARAGGEFITIEKDKIEFMDVSPKQLVSVAAALIPFLEHDDANR
                     ALMGSNMQRQSVPLLRTEPPLVGTGLEGIVARDSGAVVACKRDGIVDTVDARRIIVRV
                     QTVGEDGETGDFGADIYPLLKFRRSNQNTCINQKPIVKEGDRVVAGQVLADGPCTSQG
                     ELALGRNILVAFMPWRGYNYEDAILISEKMVKEDYYTSIHIEEFEIEARETKLGAEEI
                     TKDIPNVSEHALRDLDESGIVRIGANVKPGDILVGKVTPKGET"
BASE COUNT          316 a          567 c          541 g          229 t
ORIGIN      
        1 cttcttcaac cccgagcgct acgacctctc caaggtcggc cgcctcaagc tgaactacaa
       61 gttcggcctg gaggagccgc tcgacaacca ggtcctcacc aagcgcgacg tcctcgaggt
      121 ggtccgcttc ctcatcgacc tgaagaacgg gtcgaaggag cgggacatcg acgacatcga
      181 ccacctcggc aaccgccgcg tccgcgcggt gggcgagctg ctcgagaacc agtaccgcat
      241 cggcctcgtc cgcatggagc gcgcgatcaa ggagcgcatg agcctccaag agatcgagac
      301 gctcatgccg cacgacctga tcaacgccaa gccggtcacg gcggtgatca aggagttctt
      361 cgggtcctcg cagctctcgc agttcatgga ccagacgaac ccgctctccg aggtgacgca
      421 caagcgccgg ctctcggccc tcgggccggg cggcctgtcg cgcgagcgcg ccgggttcga
      481 ggtgcgcgac gtccacgcca cgcactacgg ccgcatctgc ccgatcgaga cgccggaagg
      541 cccgaacatc gggctcatct cgtcgctctc gtgcttcgcc cgcatttccg agttcgggtt
      601 catcgagagc ccgtacaaga aggtccggaa cgggcgcgtc ctcgaccact acgagatcgt
      661 cacggtcgga gactccgggt ggaagaaggg ccagatcgtc gaggccgagg agctcgaaga
      721 ggcggtcctc cgcctgaaga agggccacaa gcggcagccg gtcggccgcc cgacggcctt
      781 cttccagccc gcctgggaag aggagaacta cacgatcgcc caggcgaacg ccgaggtcga
      841 cgacaagggc cggttcaccg aggaccggat cgtcgcccgc gcgggcggcg agttcatcac
      901 gatcgagaag gacaagatcg agttcatgga cgtgtcgccg aagcagctcg tttcggtcgc
      961 cgcggccctc atccccttcc tcgagcacga cgacgcgaac cgggccctca tggggtcgaa
     1021 catgcagcgc cagagcgtgc cgctgctccg cacggagccg ccgctcgtcg gcacggggct
     1081 cgagggcatc gtcgcgcgcg actccggcgc ggtcgtcgcc tgcaagcggg acgggatcgt
     1141 cgacacggtc gacgcgcgcc ggatcatcgt gcgcgtccag accgtcggcg aggacggcga
     1201 gacgggggac ttcggcgcgg acatctaccc gctcctgaag ttccgccgtt cgaaccagaa
     1261 cacctgcatc aaccagaagc cgatcgtgaa ggaaggcgac cgcgtcgtcg cgggccaggt
     1321 cctcgcggac ggcccctgca cgtcgcaggg cgagctcgcg ctcggccgga acatcctcgt
     1381 cgcgttcatg ccgtggcgcg gctacaacta cgaggacgcg atcctcatct ccgagaagat
     1441 ggtcaaggag gactactaca cctccatcca catcgaggag ttcgagatcg aggcccgcga
     1501 gacgaagctc ggcgccgagg agatcacgaa ggacatcccg aacgtctcgg agcacgcgct
     1561 ccgggacctg gacgagtccg gcatcgtgcg catcggcgcg aacgtcaagc cgggcgacat
     1621 cctcgtcggc aaggtcacgc cgaagggcga gac
//
LOCUS       DAOMFY010000494         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_43235_length_1622_cov_6.286535, whole genome shotgun sequence.
ACCESSION   DAOMFY010000494 DAOMFY010000000
VERSION     DAOMFY010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43235_length_1622_cov_6.286535"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..>1622)
                     /locus_tag="PLB01_20375"
     CDS             complement(167..>1622)
                     /locus_tag="PLB01_20375"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HQQ79707.1"
                     /translation="VGSRVLAPVFGTSLFVWGALITTFLAALAAGYALGGRLADRRPE
                     PELLSAVLLAAGALVIILFAAPDAVLAAASGAPVPDRFRALLAAAVLFAPPSILMGAV
                     TPFAVRLAAKDLALVGSAAGRLSAVSTVGSILGAFAAAFFLIPTFPTRPILFGIGAAL
                     LVSALLVPAGRLLRRAAWAGFLGAAGALVFLVGTGLAKAPGPAGTLLLEKETAYHRIR
                     VVDQGLRRALYFDARLQGYAPARPGITLGLNYTDGLVLSLAFAPKARRVVVIGLGAGM
                     IPTLLAARAPEIATTSIEIDPEVVAVARKYFAFAPDANDRVIVGDGRREMDRQVDGAD
                     VILVDAYFSDSLPFHLVTKEFDELCARKLGPDGVVAVNFGGELTGSSNQLFWAALRTL
                     SEVFPRVYVFCAELKAGTVPFKSTAIVIATRSPRRLDPGSIADRAREVAERLGRPAVL
                     EFAGRLYEGEVKTAGVPVLTDAYSPTDALQHLAR"
BASE COUNT          259 a          575 c          624 g          164 t
ORIGIN      
        1 ctgcccgtga cccgcgcgcc gagcgccgcg agagcccgcg cgtcggcctc cacggccgtc
       61 gacgcctcgg cccgcgccgc gagccgggcc tgcagcgacg ggggcgtcgg cttcgcgcag
      121 gccgggccga gaagcacgag gagcgcggcg agggcggggc gcaccttcat cgcgcgaggt
      181 gctggagggc gtccgtcggg ctgtacgcgt ccgtcaggac cggcacgccg gcggtcttca
      241 cctcgccctc gtagaggcgc cccgcgaact cgaggacggc gggcctcccg agccgctcgg
      301 cgacctcacg ggcgcggtcc gcgatcgacc ccgggtccag ccgccggggc gagcgcgtcg
      361 cgatcacgat cgcggtcgac ttgaacggaa ccgtgccggc cttcagctcg gcgcagaaca
      421 cgtagacgcg cggaaagact tccgaaagcg tcctgagcgc ggcccagaag agctggttgc
      481 tggatcccgt cagctcgccc ccgaagttca cggcgacgac cccgtccggg cccagcttcc
      541 gcgcgcagag ctcgtcgaac tccttcgtga cgagatggaa cggcaggctg tcggaaaaat
      601 aggcgtccac gaggatcacg tccgcgccgt ccacctggcg gtccatttcg cggcggccgt
      661 cgccgacgat cacgcggtcg ttcgcgtccg gcgcgaacgc gaaatacttt cgcgcgaccg
      721 cgacgacctc ggggtcgatc tcgatcgacg tcgtcgcgat ctcgggcgcg cgcgcggcca
      781 gaagagtcgg aatcatgccg gccccgagtc cgatgacgac gacccgccgg gccttcggcg
      841 cgaacgcgag cgagagcacg agcccgtccg tgtagttgag cccgagcgtg attccgggcc
      901 tcgccggggc gtagccctgg agccgggcgt cgaaatagag ggcccggcgc agcccctggt
      961 ccacgacgcg gatgcggtgg taggccgtct ccttttcgag gaggagcgtg ccggccggcc
     1021 cgggcgcctt cgcgaggccg gtcccgacga gaaacacgag cgcgccggcg gctccgagga
     1081 aaccggccca cgccgcgcgg cgcaggagcc gcccggcggg gacgagcagc gccgacacga
     1141 gcagagcggc cccgattccg aagaggatcg ggcgggtggg aaaggtcggg atcaggaaga
     1201 acgccgccgc gaacgcgccg aggatcgagc cgacggtcga cacggccgag agccggcccg
     1261 cggccgaccc gacgagcgcg aggtccttcg cggcgagccg cacggcgaac ggcgtgacgg
     1321 cgcccatgag gatgctcgga ggagcaaaga gcacggcggc cgcgaggagc gcacggaacc
     1381 ggtccggcac cggcgccccg ctggcggccg cgagaacggc gtcgggggcg gcgaagagga
     1441 tgatcacgag cgcgcccgcc gcgaggagca cggccgagag cagctccggc tcgggccggc
     1501 ggtccgcgag ccggccgccg agggcgtagc ccgcggcgag ggccgcgagg aaggtcgtga
     1561 tgagcgcgcc ccacacgaag agcgacgtcc cgaagaccgg ggcgaggacg cggctcccga
     1621 cg
//
LOCUS       DAOMFY010000495         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_43587_length_1615_cov_5.996154, whole genome shotgun sequence.
ACCESSION   DAOMFY010000495 DAOMFY010000000
VERSION     DAOMFY010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43587_length_1615_cov_5.996154"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PLB01_20380"
     CDS             <1..1122
                     /locus_tag="PLB01_20380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ79708.1"
                     /translation="YLAAGADAVILGEPEHAMADLAALWAEDPAAPLDTVRGLALAGG
                     TGLDGAPAVRRTPMRPFLEDLDALPLPAWDLVDAAAYRMAWTRAHGRLSWNVVTTRGC
                     PFHCNWCAKPLWGTRYAQRSPANVADEVRLLADTVRPDHLWFADDIFGLSPRWTESYA
                     EALAARRVRIPFTMQSRVDLMTPSAVAALAAAGAEEVWMGVESGSQRILDAMDKGTRI
                     SQVRTATRLLRSHGIRVAWFLQLGYTGETWADLLATRDLVRTERPDDVGVSVAYPLPG
                     TLFYERVKEQLGARTNWSDSDELAMMFRGTYTSAFYRRVRDVLHEEVAALSAGTNAAA
                     EARLACDETWRELDETENLHRSEPPGVEASGPRLRLARG"
     gene            1140..>1615
                     /locus_tag="PLB01_20385"
     CDS             1140..>1615
                     /locus_tag="PLB01_20385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ79709.1"
                     /translation="MPLPAAVRAFDALAPGFDAAFAGWTSVAAQRRAVRRELLAAFPP
                     GARLLELGGGTGEDALFLAAEGRRVHVTDGAPAMVRRTREKARAAGLDEEVTAEALTL
                     ETLDAWAATRTGVRFDGAYSNFASLNCVTDHASVAHGLARVLPPGARALLVVFGPAS"
BASE COUNT          201 a          628 c          575 g          211 t
ORIGIN      
        1 tacctcgccg ccggcgccga cgccgtgatc ctcggcgagc ccgagcacgc gatggccgat
       61 ctcgccgcgc tctgggcgga ggaccccgcg gcacccctcg acaccgtgcg cggcctcgcg
      121 ctggcgggcg gaaccggcct cgacggtgcg ccggccgtcc gacgcacgcc catgcggccc
      181 ttcctcgaag acctcgacgc cctgcctctg cccgcctggg acctcgtcga cgcggcggct
      241 taccgcatgg cgtggacgcg ggcgcacggg cgcctctcgt ggaacgtcgt gacgacgcgc
      301 ggctgcccct tccactgcaa ctggtgcgcg aagccgctct ggggcacgcg ctacgcacag
      361 cgcagccccg cgaacgtcgc ggacgaggtc cgcctgctgg cggacacggt gcggcccgac
      421 cacctctggt tcgccgacga catcttcggc ctttcgcccc gctggacgga gtcctacgcg
      481 gaggcgctcg cggcgcggcg cgtgcggatt ccgttcacga tgcagtcgcg cgtcgacctc
      541 atgacgccgt cggccgtcgc ggcgctcgcc gccgccggcg ccgaggaggt ctggatgggc
      601 gtcgagtccg gctcgcagcg gatcctcgac gcgatggaca agggcacgcg catcagccag
      661 gtccggacgg cgacccggct cctccggagc cacgggatcc gcgtcgcgtg gttcctccag
      721 ctcggctaca cgggcgagac gtgggcggac ctcctcgcga cgcgcgacct cgtccgcacc
      781 gagcgcccgg acgacgtggg cgtctcggtg gcgtacccgc tgccgggaac gctgttctac
      841 gagcgggtga aggagcagct cggggcgcgc acgaactgga gcgacagcga cgagctcgcg
      901 atgatgttcc gcgggacgta cacgagcgcg ttctaccggc gcgtgcgcga cgtcctgcac
      961 gaagaggtcg cggcgctttc cgccgggacg aacgccgcgg ccgaggcgcg actggcctgc
     1021 gacgagacgt ggcgcgaatt ggacgagacc gagaacctgc atcgctccga gccccccggg
     1081 gtcgaggcct cggggccgcg cctccgcctc gcgcgcggat gacccgcggg agccgcgtga
     1141 tgcccctccc cgccgccgtc cgtgccttcg acgccctcgc cccgggcttc gacgccgcct
     1201 ttgccggatg gacctccgtc gccgcgcagc gccgcgccgt gcgccgcgag ctcctcgccg
     1261 ccttcccgcc gggggcacgc ctcctcgagc tcggaggcgg caccggcgag gacgctctct
     1321 tcctcgcggc agagggccgc cgcgtccacg tgacggacgg cgcgcccgca atggtccgga
     1381 gaacgcgcga aaaagcgcgg gctgccgggc tggacgaaga ggtcaccgcc gaggccctga
     1441 cgctcgagac actcgacgcc tgggccgcga cgcggaccgg agtccgcttc gacggcgcct
     1501 attcgaactt cgcgtcgctc aactgcgtga cggaccacgc gtccgtcgcg cacggcctcg
     1561 cgcgcgtgct gccgccgggc gcgcgcgccc ttctcgtcgt cttcggcccc gcatc
//
LOCUS       DAOMFY010000496         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_43842_length_1610_cov_6.320900, whole genome shotgun sequence.
ACCESSION   DAOMFY010000496 DAOMFY010000000
VERSION     DAOMFY010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43842_length_1610_cov_6.320900"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLB01_20390"
     CDS             complement(<1..487)
                     /locus_tag="PLB01_20390"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQQ79710.1"
                     /translation="MLRFERLAAAGPGPRRGRLTLDHGTVETPAFMPVGTAGTVKAAR
                     WSDLEAAGAEIVLANTYHLMLRPGGDTVRRLGGLHAFTGWNRPILTDSGGYQVMSLAG
                     SRTLSEDGVLFRSHVDGSAHALTPERAVELQLGDFGVDVAMVLVECTPWPATKDEART
                     SM"
     gene            complement(492..>1610)
                     /gene="traF"
                     /locus_tag="PLB01_20395"
     CDS             complement(492..>1610)
                     /gene="traF"
                     /locus_tag="PLB01_20395"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HQQ79711.1"
                     /translation="RAQMPFGPVTARATALGGAAVGLGPDVAGAVDNPALAPDKNFAF
                     ALSAGLVTREGGDFFAPLKVIAGNDPVKLATGSQPQSYADVVAALRTLADPGNGMLGN
                     GNVSLAAAHGGWELSFTDRAFSGTFVRADLVHTALGANPATSIAFNKSATVFRGLELK
                     DLALSKSVSFLEGTITLGAAVHALWGTTYVAEESAFTTDAGAGPWSFAQTSLDGLAQS
                     HTDWSADLGALVSLGPVHVGAVWRGINKPSFPYAEGAPAAERGQSVTWGPQLRVGASV
                     HVPLIGLTFAADADVTANDTLVEGLKSREIGGGVEWTIVALVVRAGASINLESPDKKP
                     ALTGGAGVVIGPAKVDLSGVYRTADGALGLVATARFGI"
BASE COUNT          219 a          582 c          601 g          208 t
ORIGIN      
        1 ccatggacgt ccgcgcctcg tccttcgtcg ccggccatgg cgtgcactcg acgaggacca
       61 tcgcgacgtc gacgccgaag tcccccagct gcagctcgac ggcccgctcg ggcgtgagcg
      121 cgtgggccga tccgtccacg tggctgcgga agaggacgcc gtcctccgag agcgtccggc
      181 tgcccgcgag cgacatcacc tggtaaccgc ccgagtccgt gaggatcggc cggttccagc
      241 cggtgaacgc gtgcaggcct ccgagccgcc gcacggtgtc gccgcccggc cggagcatga
      301 ggtggtacgt gttcgcgagg acgatctccg ccccggccgc ctcgaggtcg ctccagcgcg
      361 ccgccttgac cgttcccgcg gtcccgacgg gcatgaacgc cggcgtctcg accgtgccgt
      421 gatccagagt caggcgaccc cggcgcgggc ccgggccggc ggccgcgagg cgttcgaagc
      481 ggagcatcgc gtcagatgcc gaagcgggcg gtcgcgacga ggccgagcgc cccgtccgcc
      541 gtacggtaca cgccgctgag gtcgaccttc gcggggccga tcacgacgcc cgcgcctccc
      601 gtgagcgccg gcttcttgtc gggcgactcg aggttgatcg acgccccggc gcggacgacg
      661 agggcgacga tcgtccactc gacgccgccg ccgatctcgc ggctcttcag gccctcgacg
      721 agggtgtcgt tcgccgtcac gtcggcgtcg gccgcgaagg tgaggccgat gagcgggacg
      781 tggacggacg cgccgacgcg cagctgcgga ccccacgtca cggactgccc gcgctcggcc
      841 gcgggcgcgc cctccgcgta cgggaaggac ggcttgttga tgccccgcca gaccgcgccg
      901 acgtggaccg ggccgagcga cacgagagcg ccgaggtcgg cggaccagtc ggtgtgcgac
      961 tgggcgaggc cgtcgagaga cgtctgggcg aagctccagg gcccggcgcc cgcgtccgtc
     1021 gtgaaggcgg actcttccgc gacgtacgtg gtcccccaga gcgcgtgcac ggccgcgccg
     1081 agcgtgatcg tgccctcgag gaacgacacc gacttcgaga gcgcgaggtc cttcagctcg
     1141 aggccgcgga agacggtcgc ggacttgttg aaggcgatcg acgtcgcggg gttcgccccg
     1201 agagccgtgt ggacgaggtc ggcccggacg aacgtgccgc tgaacgcgcg gtccgtgaac
     1261 gacagctccc agcctccgtg cgcggctgcg agcgagacgt tcccgttccc gagcatcccg
     1321 ttgcccgggt cggcgagcgt ccggagcgcg gcgacgacgt ccgcatagct ctgcggctgg
     1381 gagccggtcg cgagcttcac gggatcgttc ccggcgatta ccttgagcgg cgcgaagaag
     1441 tcgccgccct cgcgcgtgac gaggcctgcc gagagcgcga acgcgaagtt cttgtcgggg
     1501 gcgagcgcgg ggttgtccac ggcgcccgcg acgtcgggcc ccagccccac ggccgcgccg
     1561 ccgagggccg tcgcacgggc cgtcacgggc ccgaagggca tctgcgcgcg
//
LOCUS       DAOMFY010000497         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_43981_length_1607_cov_7.156572, whole genome shotgun sequence.
ACCESSION   DAOMFY010000497 DAOMFY010000000
VERSION     DAOMFY010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43981_length_1607_cov_7.156572"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1274)
                     /locus_tag="PLB01_20400"
     CDS             complement(90..1274)
                     /locus_tag="PLB01_20400"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HQQ79712.1"
                     /translation="MSENTRKPGFATTCIHAGQEPEPVTGAVTVPIFQTSTYVQEAFG
                     KPKGGYEYARTKNPTRLALEQQLAALEGGAVAHAFASGMAAETTLMLAFVKAGDHVLV
                     SRNTYGGTYRFFSKVLANFGVTFAYVDTTDAEAVRKAVTPETRMLFVETPTNPTMEIS
                     DLKALADVAKAEGSRIGAKIRVVVDNTFATPFFQRPLSLGCDVVVHSTTKYLNGHSDS
                     VGGAVISADAADGEKIGFTQNAAGGILSPFDSFLVSRGIKTLPVRMKAHDANGRAVAA
                     WLSRHPKVLKVFYPGLPGHPGHAVAGRQMSGYGGMISFDLGSEAAARRLLDRVVVCAL
                     GESLGGVESLISHPATMTHASVEPDERLRRGISPGLVRVSVGIEDVEDILADLENALA
                     GV"
     gene            complement(1330..>1607)
                     /locus_tag="PLB01_20405"
     CDS             complement(1330..>1607)
                     /locus_tag="PLB01_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HQQ79713.1"
                     /translation="KSIPCIDCGAPFIFSANEQEFYAQKGFSNEPKRCKNCRDKRKAG
                     GFGEGGARQLYNVTCDACGKTTQVPFNPTNGKPVYCRDCYRERRPAR"
BASE COUNT          225 a          540 c          582 g          260 t
ORIGIN      
        1 cccgcggacg acgtgccggc gctctcgccg cgcgcgctcg ccgcgctcgc ggccgcgctc
       61 gcgatcctcg gcgccctcgc cctgaagcgc tagacgccgg ccagcgcgtt ttcgaggtcc
      121 gcgaggatgt cctcgacgtc ctcgatgccg accgagacgc gcacgagccc cgggctgatg
      181 ccgcgcctca ggcgctcgtc gggctcgacg ctcgcgtgcg tcatcgtcgc cgggtgcgag
      241 atgaggctct cgacgccgcc cagcgactcg ccgagcgcgc acacgacgac gcggtcgagg
      301 aggcgcctgg ccgcggcctc cgagccgagg tcgaacgaga tcatgccgcc gtagcccgac
      361 atctggcgcc ccgcgacggc gtggcccggg tgcccgggca ggcccgggta gaagaccttc
      421 agcaccttcg ggtggcgcga gagccacgcg gcgaccgcgc ggccgttcgc gtcgtgcgcc
      481 ttcatgcgca cgggaagggt cttgatcccg cgcgagacga ggaaggaatc gaacggcgac
      541 aggatgccgc ccgcggcgtt ctgcgtgaac ccgatcttct cgccgtccgc cgcgtccgcc
      601 gagatcacgg ccccgcccac ggagtcggag tggccgttca ggtacttcgt cgtcgagtgg
      661 acgacgacgt cgcagccgag ggaaagcggc ctctggaaga acggcgtcgc gaacgtgttg
      721 tcgacgacga cgcggatctt cgcgccgatc cgcgacccct cggccttcgc gacgtcggcg
      781 agcgccttga ggtcggagat ctccatcgtc gggttcgtgg gcgtctcgac gaagagcatc
      841 cgcgtctcgg gcgtgacggc cttgcggacg gcctcggcgt cggtcgtgtc gacgtacgcg
      901 aacgtgacgc cgaagttcgc gaggaccttg gagaagaagc ggtacgtgcc gccgtacgtg
      961 ttgcggctga cgaggacgtg gtcgcccgcc ttcacgaacg cgagcatgag cgtcgtctcg
     1021 gccgccatgc cggacgcgaa cgcgtgggcg acggcgccgc cctcgagcgc cgcgagctgc
     1081 tgctcgagcg cgagacgcgt ggggttcttc gtgcgcgcgt actcgtagcc gcccttcggt
     1141 tttccgaagg cttcctggac gtaggtggac gtctggaaga tggggacggt caccgcgccc
     1201 gtgacgggct ccggctcctg tcccgcgtgg atgcaggtcg tcgcgaaacc gggtttgcgg
     1261 gtgttctcgc tcatgagagc gagactatag accgggggga aagggtccaa ccctttcccc
     1321 ccagaccccc tatcgggcgg ggcgccgctc gcggtagcag tcgcggcagt agaccggctt
     1381 gccgttcgtg gggttgaacg gaacctgggt cgtcttgccg caggcgtcgc aggtgacgtt
     1441 gtagagctgg cgcgcgccgc cctcgccgaa cccgccggcc ttgcgcttgt cgcggcagtt
     1501 cttgcaacgc ttgggctcgt tggagaagcc cttctgcgcg tagaactcct gttcgttcgc
     1561 cgagaagatg aagggggcgc cgcagtcgat acaggggatg gatttgt
//
LOCUS       DAOMFY010000498         1603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_44182_length_1603_cov_6.793282, whole genome shotgun sequence.
ACCESSION   DAOMFY010000498 DAOMFY010000000
VERSION     DAOMFY010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44182_length_1603_cov_6.793282"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLB01_20410"
     CDS             <1..333
                     /locus_tag="PLB01_20410"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQQ79714.1"
                     /translation="GGMPRGAFAAKDGDVAWVFVAGETWRIAKAGARAARAADEEHAL
                     TAPMPGRIAAVRVADGQKVEKGDVLVILEAMKMEHAVRAPRTGTVSRLAAEPGKMVSL
                     GAVLLDVT"
     gene            330..1247
                     /locus_tag="PLB01_20415"
     CDS             330..1247
                     /locus_tag="PLB01_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HQQ79715.1"
                     /translation="MSRTRVTVVEVGPRDGLQNEAAILPADVKVAFVDALSAAGLPVI
                     EATAFVSPKAIPQLADGEDVFRRIVKRPGVRYPVLVPNEKGLDRALAAGVREIAVFTA
                     ASETFNRRNINASIDESFARFAPVLARAKDAGLRVRGYVSCAFGCPYEGPVEPEAVAR
                     VASRLVEIGCAEVSVGDTIGIGVPTQVPDVVGRTVEAGVSIDALALHFHDTRGTALAN
                     VAEGLRAGVRIFDSSAGGLGGCPYAPGAAGNVATEDLLFLLDGMGYETGADLASVAGA
                     SRVLSAAIGRRPASRVFQALEAAAAMRPV"
     gene            1262..>1603
                     /locus_tag="PLB01_20420"
     CDS             1262..>1603
                     /locus_tag="PLB01_20420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ79716.1"
                     /translation="MPSRILLADDSLTIRKVVELTFSGSEFELKAVGNGDEAVALLDE
                     FQPDIVLADAVMPGKAGYDVCEEVKKRPGGRYVPVVLLTGTFEPFDKPRSERVGADSV
                     VTKPFDSQGLAA"
BASE COUNT          214 a          567 c          591 g          231 t
ORIGIN      
        1 ggcgggatgc cgcgcggcgc gttcgcggcg aaggacggcg acgtcgcctg ggtcttcgtc
       61 gccggcgaga cgtggcgcat cgcgaaggcg ggcgcccgcg ccgcccgagc ggccgacgag
      121 gagcacgcgc tcacggcgcc catgccgggc cgtatcgcgg ccgtgcgggt ggcggacggc
      181 cagaaggtcg agaagggcga cgtcctcgtg atcctcgaag ccatgaagat ggagcacgcc
      241 gtgcgggcgc cgcggacggg aacggtgtcg cggctcgccg ccgagcccgg gaagatggtg
      301 agcctcggcg ccgttctcct cgacgtcaca tgagccgcac gcgcgtcacc gtcgtcgagg
      361 tcggtccccg ggacggcctg cagaacgagg ccgcgatcct cccggcggac gtgaaggtcg
      421 cgttcgtcga cgcgctctcg gcggccggcc tgcccgtgat cgaggcgacc gcgttcgtct
      481 cgccgaaggc gatcccgcag ctcgcggacg gcgaggacgt cttccgccgg atcgtgaagc
      541 gcccgggcgt ccgctacccc gtgctcgtcc cgaacgagaa ggggctcgac cgcgccctcg
      601 cggcgggcgt ccgggagatc gcggtcttca cggcggcctc cgaaacgttc aaccgccgga
      661 acatcaacgc ctccatcgac gagtcgttcg cgcgattcgc accggtcctc gcccgcgcga
      721 aggatgcggg actgcgcgtg cgcggctacg tctcgtgcgc gttcggctgc ccgtacgaag
      781 gcccggtcga accggaggcg gtcgcacgcg tcgcgtcgcg gctcgtcgag atcgggtgcg
      841 ccgaggtctc cgtcggggac acgatcggga tcggcgttcc gacacaggtg ccggacgtcg
      901 tcggacggac cgtcgaggcc ggcgtttcga tcgacgcgct cgcgctgcac ttccacgaca
      961 cccgcgggac ggccctcgcg aacgtggccg agggcctgcg cgcgggcgtg cggatcttcg
     1021 actcttcggc gggcggtctc ggcggctgcc cgtacgcgcc gggcgcggcg ggaaacgtcg
     1081 cgaccgagga cctgctcttt cttctcgacg ggatgggcta cgaaacgggc gcggacctcg
     1141 cgtccgtcgc cggcgcgtcg cgcgtcctct ccgccgcgat cgggcggcgc ccggcttcgc
     1201 gcgtctttca ggccctcgag gcggccgccg cgatgcgtcc ggtgtaaact cccacccaac
     1261 catgccttcc cgaatccttc tggcggacga cagcctgacc atccgcaagg tggtggagct
     1321 gactttctcg ggcagcgagt tcgagctcaa ggcggtcggc aacggcgacg aggccgtggc
     1381 cctcctcgac gagttccagc ccgacatcgt cctcgcggac gccgtcatgc cgggcaaggc
     1441 cggctacgac gtgtgcgagg aggtgaagaa gcgcccgggc gggcggtacg ttccggtcgt
     1501 gcttctcacc ggcacgttcg agccgttcga caagccgcgg tccgaacgcg tcggcgccga
     1561 ttcggtcgtc acgaagccgt tcgactcgca gggactcgcc gcg
//
LOCUS       DAOMFY010000499         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_44408_length_1599_cov_5.417098, whole genome shotgun sequence.
ACCESSION   DAOMFY010000499 DAOMFY010000000
VERSION     DAOMFY010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44408_length_1599_cov_5.417098"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(504..1157)
                     /locus_tag="PLB01_20425"
     CDS             complement(504..1157)
                     /locus_tag="PLB01_20425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ79717.1"
                     /translation="MKASHRLPIPGLDALRRSLRRRLLGRGKPRSAEVWNDQYRAGTW
                     DGLVAETERHACVAELIRRYAPPAPLVLDVGCGHGALARQLRTGAGGRYVGIDISEEA
                     VRRAEEGAVPEESFRAFDVEGGPPDFLAPGSVDAAVFAEVLYYLDDPLATLRRFLPLL
                     SPRGIFVFSLWKPARHRVLRRRLAREYDEACAREVGRGETWRISFCVPRGRSLPVPA"
     gene            1217..>1599
                     /locus_tag="PLB01_20430"
     CDS             1217..>1599
                     /locus_tag="PLB01_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79718.1"
                     /translation="MARLASREPAAPPTEDGAESRLVSAGVVLALVLPFISFSTASSG
                     ALRESKLLAQALGSGLVLFGLAAPGAWGFLRGEASSRTARVPPLALAAALAFACASAA
                     ANAHVVDPLVLGALLSPLALAAAGAS"
BASE COUNT          201 a          593 c          556 g          249 t
ORIGIN      
        1 gcgctgcggc gcgtgcccgc tccgctcctc ctgccgcgcg gccgcgggcg ggacgccgga
       61 ggcgtttccg cgggcggccg cccgcgtttc gcccaggacg attcatctcg tcggcgcgat
      121 cgtggagaga ggaaagaaga ttctgctggt tgaagatcag gagatggtcg ccggccacct
      181 cgtccctccg atgctgcgcg tgcgcgccgg ggacgggtct gcaaattctc ttagaaggtg
      241 gtggaagcgt ctcgccgggc gtgacgtcag cgcaatcgag ccggtggcgg tgcttcgcca
      301 ttccgtactc gagaggcgct atcggatttc cctcttcacc ttcaaagaaa gtcttcttcc
      361 cccgtcctcc atctctcgtc cgcgtcgcgg gcgccgcgca gcgtcccgtc tctgtgctct
      421 ctccccgagc cagatctccc gtgtcgccca cggcggcctg ctcctgaagg tgctcgccgc
      481 gtggcgcgcg caccgcgccc ggctcaggcg ggaaccggaa gagagcgtcc ccgcggaacg
      541 cagaagctga tccgccacgt ctcgccgcgc ccgacttccc gggcgcaggc ttcgtcgtac
      601 tccctggcga ggcgccggcg aaggacccgg tggcgggccg gcttccagag ggagaagacg
      661 aagatgccgc gcggcgacag gagcggcaga aagcggcgga gcgtcgcaag cgggtcgtcg
      721 aggtagtaga ggacttcggc gaagaccgcc gcgtcgaccg atccgggcgc gaggaagtcg
      781 ggcggccccc cttcgacgtc gaacgcacgg aacgactcct ccggaacggc gccctcttcc
      841 gcgcgccgga ccgcctcctc cgaaatgtcg atccccacgt agcgtcctcc cgcgccggtt
      901 ctcagctggc gggcaagcgc gccgtgcccg cacccgacgt ccaggaccag aggcgcgggc
      961 ggagcgtacc gacgaatgag ctccgcgacg caagcgtgcc gctcggtctc ggcgacgagc
     1021 ccgtcccacg tgccggcccg gtactggtcg ttccacacct cggccgacct cggcttgccg
     1081 cggccgagca ggcggcgcct gagcgatcgg cgcagcgcgt cgaggcccgg gatcgggaga
     1141 cggtgactcg ctttcacgga gacgcctggg cagcttatcc cacgggccgc gcgccgtttc
     1201 gttatcctgc ggcgcgatgg cgcgcctcgc ttcccgggag cccgcggcgc cgcccacgga
     1261 agacggggcc gagtcacggc tcgtctccgc gggcgtcgtg ctcgcgctgg ttctcccgtt
     1321 catttccttc tcgacggcct cgagcggcgc actgcgcgaa agcaagcttc tcgcgcaagc
     1381 gctcggctcg ggcctcgtcc tcttcggact cgcggcgccc ggggcatggg gcttcctgcg
     1441 gggcgaggcg agcagtcgga cggcccgcgt cccgcctctc gcgctcgccg cggctctggc
     1501 tttcgcgtgc gcgtcggcgg ccgccaatgc ccacgtcgtg gacccgctcg tcctcggcgc
     1561 ccttctctcg ccgctcgcgc tcgccgccgc gggagcgtc
//
LOCUS       DAOMFY010000500         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_44704_length_1593_cov_7.447334, whole genome shotgun sequence.
ACCESSION   DAOMFY010000500 DAOMFY010000000
VERSION     DAOMFY010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44704_length_1593_cov_7.447334"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..77
                     /locus_tag="PLB01_20435"
     tRNA            3..77
                     /locus_tag="PLB01_20435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Gln,seq:ttg)
     gene            94..1047
                     /locus_tag="PLB01_20440"
     CDS             94..1047
                     /locus_tag="PLB01_20440"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQQ79719.1"
                     /translation="MERFAYGDLKVFGGRAHPALTGEICRNLGREAGRVTLYNFSDGE
                     NYCQIDENVRGADVFIVQPTGPPANHNIMELLIMLDAFKRSSASRVTAVLPYYGYARQ
                     DRKDKPRVPITAKLTADLITAAGADRALCIDLHAAQIQGFFNIPVDHLFAAPVLLDAI
                     RDSSLDPLTVVSPDAGGVERARAFAKRLGAGLAIVDKRRKGKNEVEVLHVIGDVKDRN
                     VIVVDDIIDTAGTLINTVIALKDSGARRIFAGCVHAVLSGPAIDRINGSPIERIFCAN
                     TLPLDEKLARCPLLTPLSIAPLLAEAIQRIHQGASVSSLFV"
     gene            1116..>1593
                     /locus_tag="PLB01_20445"
     CDS             1116..>1593
                     /locus_tag="PLB01_20445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQQ79720.1"
                     /translation="MLQEDIVVEVEKRDVTGKNAANRLRAQDLIPAVLYGGTKEMGNF
                     PLTVPRKTLATLLNKGLHGNAIFKLQLKGTKDQRHVMIRDLALDPITRAMLHVDFVRV
                     LLDKKLRVKSDVEVVGVAAGVKEGGILNIVTHEILIECLPADIPESIKIDVSEMKIH"
BASE COUNT          298 a          594 c          469 g          232 t
ORIGIN      
        1 gatggggagt cgacaagtgg taagtcagcg gtctttggaa ccgcctaccg gaggttcgaa
       61 tcctccctcc ccagccaact ctcctgaacg acgatggaac gattcgcata cggtgacctg
      121 aaggttttcg gcggccgggc ccacccggcg ctgacgggcg agatctgccg caacctcggg
      181 cgcgaggcgg gccgggtcac gctctacaac ttctccgacg gcgagaacta ctgccagatc
      241 gacgagaacg tgcgcggcgc ggacgtcttc atcgtccagc ccacgggccc cccggcgaac
      301 cacaacatca tggaactcct catcatgctc gacgccttca agcgctcctc ggcgtcgcgc
      361 gtgaccgccg tgctcccgta ctacgggtac gcgcggcagg accgcaagga caagccccgc
      421 gtcccgatca cggcgaagct cacggccgac ctcatcacgg ccgcgggcgc cgaccgcgcg
      481 ctgtgcatcg acctccacgc cgcgcagatc cagggcttct tcaacatccc cgtcgaccac
      541 ctcttcgcgg cgcccgtcct gctcgacgcg atccgcgact cgagcctcga cccgctcacg
      601 gtcgtctcgc cggacgccgg cggcgtcgag cgcgcccgcg cgttcgccaa gcggctcggc
      661 gccggtctcg cgatcgtcga caagcggcgc aaggggaaga acgaggtcga ggtcctgcac
      721 gtcatcggcg acgtcaagga ccgcaacgtc atcgtcgtgg acgacatcat cgacacggcc
      781 gggacgctca tcaacaccgt catcgccctg aaggactccg gcgcgcgccg catcttcgcg
      841 ggctgcgtcc acgccgtcct ctccggcccc gccatcgacc gcatcaacgg cagcccgatc
      901 gagcgcatct tctgcgcgaa cacgcttccg ctcgacgaga agctcgcccg ctgcccgctc
      961 ttgacgccgc tctcgatcgc cccgctcctc gccgaggcga tccagcgcat ccaccaggga
     1021 gcctccgtca gctcgttgtt cgtctagacc cgccaacagg ccggaccgca cgccggggaa
     1081 attccttccc gaccgtgcgc ccgaagaaag gaaacatgct ccaggaagac atcgtcgtcg
     1141 aagtcgaaaa gagggacgtc accggaaaga acgccgcgaa ccgcctccgg gcccaggacc
     1201 tcatccccgc cgtgctctac ggcggcacca aggagatggg gaacttcccc ctcacggtgc
     1261 cgcgcaagac cctcgcgacg ctcctcaaca agggcctcca cggcaacgcg atcttcaagc
     1321 tgcagctcaa gggcacgaag gatcagcggc acgtcatgat ccgcgacctc gcgctcgacc
     1381 cgatcacgcg cgcgatgctg cacgtggact tcgtccgcgt cctcctcgac aagaagctcc
     1441 gcgtcaagag cgacgtcgag gtcgtggggg tcgcggcggg cgtcaaggaa ggcggcatcc
     1501 tgaacatcgt cacccacgag atcctcatcg agtgccttcc ggccgacatc ccggagtcga
     1561 tcaagatcga cgtctccgaa atgaagatcc acg
//
LOCUS       DAOMFY010000501         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_44708_length_1593_cov_5.713914, whole genome shotgun sequence.
ACCESSION   DAOMFY010000501 DAOMFY010000000
VERSION     DAOMFY010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44708_length_1593_cov_5.713914"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PLB01_20450"
     CDS             <1..1389
                     /locus_tag="PLB01_20450"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQQ79721.1"
                     /translation="PERAAGPAVMSAEILLVEDRESLRAMLAETLSNEGYSVEAVATG
                     DEAVRRLGEGRRYGLVLTDLKMPGADGIAVLKAAVAGDPSVPVVVLTGFGTVETAVTA
                     MKSGAADFLSKPVDPDLLLLLVERHVRARRSAVARVLLAEEAGLAGMPQIVGSSRALA
                     EALERVRHAAPSDVTVLLSGESGTGKELFARALHALSPRASGPFVAVNVAALPESLVE
                     NELFGHERGAYTGATERRAGRFELADGGTLFLDEIGELPPAAQTKLLRVVEERTFLRV
                     GGTVPITVDVRLVAATNRDLAQRVKSGAFREDLFYRLDVFPVHLPPLRTRPEDIPALV
                     KSFGAAAARQVKKRDLAFSPGALERLAAHDWPGNVRELRNVIERAVLLAPGEVVRPAD
                     IVVGLPGESAVPHPSLDGTLDEAAERWKRAGEAARIRRALDETGGDKARAAEELGVNV
                     RRLVQRMKELGL"
     gene            complement(1386..>1593)
                     /locus_tag="PLB01_20455"
     CDS             complement(1386..>1593)
                     /locus_tag="PLB01_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79722.1"
                     /translation="LLLVVGRHAAAVALRRPGLAADALAAALRALAPPVVARIEGGRV
                     LLDLRSVAEEEDAELAEALAAVSG"
BASE COUNT          202 a          584 c          609 g          198 t
ORIGIN      
        1 cctgagcgcg cggccgggcc ggccgtgatg agcgcggaga tcctgctcgt cgaggaccgc
       61 gagtcgctgc gcgcgatgct cgccgaaacc ctctcgaacg agggctactc ggtggaggcc
      121 gtcgcgaccg gcgacgaggc ggtccgccgg ctcggcgagg gccgccggta cggtctcgtc
      181 ctgacggacc tcaagatgcc gggtgccgac ggcatcgcgg tcctcaaggc cgccgtcgcg
      241 ggggacccgt ccgtgcccgt cgtcgtcctc acgggcttcg ggacggtcga gaccgcggtc
      301 acggcgatga agagcggcgc ggccgacttc ctgagcaagc ccgtcgaccc ggacctgctg
      361 ctcctcctcg tcgagcgcca cgtgcgggcg cgccggtcgg cggtcgcgcg cgtcctgctc
      421 gccgaagagg ccgggctcgc gggaatgccg cagatcgtcg gctcctcgcg cgctctcgcc
      481 gaggcgctcg agcgcgtgcg ccacgcggcg ccgtccgacg tcaccgtcct cctgagcggc
      541 gaatcgggta cgggcaagga gctcttcgcc cgcgcgctcc acgcgctctc gccgcgcgcg
      601 agcgggccgt tcgtcgccgt gaacgtcgcg gcgctcccgg agagcctcgt cgagaacgag
      661 ctcttcgggc acgagcgggg cgcttacacc ggggcgacgg agcggcgcgc ggggcggttc
      721 gagctcgcgg acggcgggac gctctttctc gacgagatcg gggaactccc gcccgcggcc
      781 cagacgaaac tcctccgcgt cgtggaggag cgcacgtttc tccgcgtcgg cggcaccgtc
      841 ccgatcaccg tggacgtgcg gctcgtcgcc gcgacgaacc gcgacctcgc ccagcgcgtg
      901 aagagcggcg ccttccgcga agacctcttc taccgcctcg acgtgttccc ggtgcacctc
      961 cccccgctgc gcacgcggcc cgaggacatt ccggcgctcg tcaagagctt cggggctgcg
     1021 gcggcgcgcc aggttaagaa gcgcgacctc gcgttctcgc cgggcgccct cgagaggctc
     1081 gcggcgcacg actggcccgg caacgtgcgg gagctgcgca acgtgatcga gcgcgccgtg
     1141 ctgctcgcgc cgggcgaggt cgtgaggccc gccgacatcg tcgtggggct tccgggcgag
     1201 agcgcggttc cgcacccgtc tctcgacggg actctcgacg aggccgccga gcgctggaag
     1261 cgtgcgggcg aagccgcgcg gatccgccgc gcgctggacg agacgggcgg cgacaaggcg
     1321 cgcgcggccg aggaactcgg cgtcaacgtc cgccgcctcg tccagcggat gaaggagctg
     1381 gggctctagc cggacacggc cgcgagggcc tccgcgagct ccgcgtcctc ctcctccgcg
     1441 acgctccgga ggtcgaggag gacgcggccg ccctcgatgc gggcgacgac gggtggggcg
     1501 agggcgcgca gggccgccgc gagagcgtct gcggcgagac ccgggcggcg cagcgcgacg
     1561 gccgcggcgt ggcggccgac gacgagcaga agc
//
LOCUS       DAOMFY010000502         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_44801_length_1591_cov_5.930339, whole genome shotgun sequence.
ACCESSION   DAOMFY010000502 DAOMFY010000000
VERSION     DAOMFY010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44801_length_1591_cov_5.930339"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLB01_20460"
     CDS             <1..377
                     /locus_tag="PLB01_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79723.1"
                     /translation="REAANRAWLDRAFRLAGTDGMRGLVILTQMNLFADYAGKVYEPT
                     KVLIAEEAKRLARPVLFVHGETHLHRFDRPFRDGSGSRVGNAWRLETFGSPQVGWVRV
                     TADPNDPDLFRVDPRPPEANPG"
     gene            complement(374..889)
                     /locus_tag="PLB01_20465"
     CDS             complement(374..889)
                     /locus_tag="PLB01_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQQ79724.1"
                     /translation="MAARVEAGSVVTIAYELRDADGALLDEEGSSVAYLHGGFGGIFP
                     KVEAALEGAGLGHEVALSLEPEDAFGDYDPGLLRVEPRESFPDVLLEGMRFEGVPGEG
                     TEDDGRIYTVTGIAEDQVVVDANHPFAGERVWFRCTVTGVRPATDDELRHGHAHGEHA
                     PEDDPPGVAPR"
     gene            complement(873..1352)
                     /locus_tag="PLB01_20470"
     CDS             complement(873..1352)
                     /locus_tag="PLB01_20470"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQQ79725.1"
                     /translation="MLQVGHPAPPFSLPDASMEMMDLASFRGKKNVVLYFYPRDGTPG
                     CTIQAIDFSDREPEFAKLDTVVFGISRDDVLSHQSFRDEQGLSVQLLADTEAEVCRLY
                     DVLRDKTVDGRAKSCVERSTFVIDKKGILRHVLRNVNPKDHADAVLDCVKTINGRAR"
     gene            1392..>1591
                     /locus_tag="PLB01_20475"
     CDS             1392..>1591
                     /locus_tag="PLB01_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQQ79726.1"
                     /translation="MARKTHPAAGRSAGDGPRFPGRMGARSFLARHWQKRPLLVRGAF
                     PGFRDPLGPGEVLALAGSSEAES"
BASE COUNT          227 a          572 c          555 g          237 t
ORIGIN      
        1 gccgggaggc cgccaaccgc gcgtggctcg accgggcctt ccggctcgcc ggcaccgacg
       61 gcatgcgcgg cctcgtgatc ctcacccaga tgaacctctt cgcggactac gccggaaagg
      121 tctacgagcc gacgaaggtc ctgatcgccg aggaggcgaa gcggctcgcg cgcccggtgc
      181 tcttcgtgca cggcgagacg cacctgcatc ggttcgaccg ccccttccgc gacggctccg
      241 gctcgcgcgt gggcaacgcg tggcggctgg agaccttcgg cagcccgcaa gtgggctggg
      301 tgcgcgtgac ggcggacccg aacgacccgg acctcttccg cgtcgacccc cgcccgccgg
      361 aagcgaaccc cggctagcgc ggcgcgacgc ccggcggatc gtcctccggc gcgtgctcgc
      421 cgtgggcgtg gccgtggcgc aactcgtcgt cggtcgcggg cctcacgccg gtcaccgtgc
      481 agcggaacca cacgcgctcg cccgcgaagg gatggttggc gtcgacgacg acctggtcct
      541 ccgcgatgcc ggtcaccgtg tagatgcgcc cgtcgtcttc ggtgccctcg cccggcacgc
      601 cctcgaagcg catgccctcc aggagcacgt cgggaaagga ctcccgcggc tcgacgcgca
      661 gcagcccggg atcgtagtcg ccgaaggcgt cctcgggctc gagcgagagg gccacctcgt
      721 ggccgaggcc cgcgccttcc agggccgcct ccaccttcgg gaagatgccg ccgaagccgc
      781 cgtgcaggta ggcgacgctc gagccctcct cgtcgaggag cgcgccgtcc gcgtcgcgca
      841 gctcgtaggc gatcgtgacg accgagccgg cctcaacgcg cgcggccatt gatcgtcttg
      901 acgcaatcga ggacggcgtc cgcgtggtcc ttgggattca cgttgcgcag gacatgccgc
      961 aggatcccct tcttgtcgat cacgaaggtc gagcgctcga cgcacgactt cgcgcggccg
     1021 tcgacggtct tgtcgcgcag aacgtcgtag agccggcaca cctcggcctc ggtgtccgcg
     1081 agcagctgca ccgacaggcc ctgctcgtcg cggaagctct ggtgcgagag cacgtcgtcg
     1141 cgcgagatgc cgaagacgac ggtgtcgagc ttcgcgaatt ccggctcgcg gtcgctgaag
     1201 tcgatcgcct ggatggtgca accgggcgtg ccgtcccggg gatagaaata gagcacgacg
     1261 ttcttcttcc cgcggaagct cgcgagatcc atcatctcca tgctcgcatc cggcagcgag
     1321 aacggcggtg cggggtgtcc aacctgtaac atgacgctcc tcgacggtcc gaccgattct
     1381 agcggaattt catggcacgc aagacccatc ccgccgcggg gcgaagcgcg ggcgacggcc
     1441 cgcgctttcc ggggcgcatg ggcgcgcggt cgttcctcgc ccgccactgg cagaagcggc
     1501 cgctgctcgt gcgcggcgcg tttcccgggt tccgcgatcc cctgggaccc ggggaggtgc
     1561 tcgcgctggc gggctcgagc gaggccgagt c
//
LOCUS       DAOMFY010000503         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_44802_length_1591_cov_5.745443, whole genome shotgun sequence.
ACCESSION   DAOMFY010000503 DAOMFY010000000
VERSION     DAOMFY010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44802_length_1591_cov_5.745443"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLB01_20480"
     CDS             <1..327
                     /locus_tag="PLB01_20480"
                     /inference="COORDINATES: protein motif:HMM:NF026044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79727.1"
                     /translation="PADIPESIKIDVSEMKIHDSFRVSDLKVGDKIKVLENADRVLAH
                     VGVPKAEEVVAAVAAVPGAEGAAAATSTEPEVIKKGKKDEEGAEPAAGGAKAAAAPKA
                     EKKDKK"
     gene            348..941
                     /gene="pth"
                     /locus_tag="PLB01_20485"
     CDS             348..941
                     /gene="pth"
                     /locus_tag="PLB01_20485"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQQ79728.1"
                     /translation="MVGLGNPGDRYSGTRHNVGFDVVHAFAEKAAGGPPRLDRLDCRA
                     LTGRVRVGNRPVLVAKPQTYMNASGESVKGLAVKYNIPLEDLVIVSDDAALPVGAIRI
                     RRSGSSGGQKGLQNVIDGFGTNEFPRLRLGVRGENFQPGEPLDTYVLSRFAKKERPVI
                     DAAIATACDALAVIVTDGLDAAMNRFNRLPEESSPAN"
     gene            1031..1471
                     /gene="rpsF"
                     /locus_tag="PLB01_20490"
     CDS             1031..1471
                     /gene="rpsF"
                     /locus_tag="PLB01_20490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQQ79729.1"
                     /translation="MATPNTQRQYDMVFLVVPEKDEAGAQGVVEEFRKLLVDHGATIE
                     KDETMGRRRLAYTIKKKNEATYHNFLFRGSSACVAEVQRKLRLSEDILRYLTVRIDEE
                     VKHGLKVARNTKPRRPRPEMSDAPMAAPAAPAPSAAPEAAEGRE"
BASE COUNT          299 a          571 c          513 g          208 t
ORIGIN      
        1 ccggccgaca tcccggagtc gatcaagatc gacgtctccg aaatgaagat ccacgactcg
       61 ttccgcgtct ccgacctcaa ggtcggcgac aagatcaagg tcctcgagaa cgcggaccgc
      121 gttctcgccc acgtcggcgt gccgaaggcc gaggaagtcg tggccgccgt cgcggccgtt
      181 ccgggcgccg agggggccgc cgccgccacg tccacggagc ccgaggtgat caagaagggc
      241 aagaaggacg aggagggcgc cgagcctgcc gcgggtggag cgaaggctgc ggccgccccg
      301 aaggccgaga agaaggacaa gaaataagca cggacggcct cttcctcgtg gtgggactcg
      361 gcaaccccgg cgaccggtat tccgggacgc gtcacaacgt cggcttcgac gtcgtgcacg
      421 cgttcgcgga gaaggccgcc ggcgggccgc cgcgcctcga ccgcctcgac tgccgcgcgc
      481 tcaccgggcg cgtgcgggtg gggaaccgcc cggtgctcgt cgccaagccc cagacgtaca
      541 tgaacgcttc gggagagtcc gtgaagggcc tcgccgtcaa gtacaacatt cccctcgagg
      601 acctcgtcat cgtttcggac gacgccgctc ttcccgtcgg ggccattcgc atccggcggt
      661 ccgggtcctc gggcgggcag aagggtctcc agaacgtgat cgacggattc ggcacgaacg
      721 agtttccgcg cctgcgcctc ggcgtgcgcg gcgagaactt ccagcccggc gagccgctcg
      781 acacgtacgt gctgtcccgt ttcgcgaaga aggagcggcc cgtcatcgac gccgcgatcg
      841 cgaccgcgtg cgacgcgctc gccgtgatcg tcacagacgg cctcgacgcc gccatgaacc
      901 gtttcaaccg tttgcccgag gaatcgtccc cggcgaactg atctgcaacc ttggctcggc
      961 accggaccag aggtccacag ccgcgcctcg cgacgggaaa acccgccgcg atccagaagg
     1021 aggaaacccc gtggccaccc cgaacaccca acgccagtac gacatggtct tcctggtcgt
     1081 ccccgagaag gacgaggccg gagcccaggg cgtcgtcgag gaattccgca agctcctcgt
     1141 cgaccacggc gcaacgatcg agaaggacga gacgatgggc cgccgccgcc tcgcctacac
     1201 gatcaagaag aagaacgagg cgacgtacca caacttcctg ttccgcggct cgtcggcctg
     1261 cgtcgccgag gtccagcgca agctgcgcct ctcggaggac atcctccgct acctgaccgt
     1321 ccgcatcgac gaagaggtca agcacggcct caaggtcgcc cgcaacacga agccgcgccg
     1381 cccgcgcccc gagatgtcgg acgcgccgat ggccgccccc gcagcccccg caccttccgc
     1441 cgcccccgag gccgcggaag ggcgcgagta ggagtccgcc atggccgcac ccgtccgcaa
     1501 gcccgccaag aagaagttct tcgtccgccg caagcgcgtc tgcaagttca ccgtcgagaa
     1561 gatcggctac atcgacttca aggacgtgaa g
//
LOCUS       DAOMFY010000504         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_46117_length_1566_cov_5.340172, whole genome shotgun sequence.
ACCESSION   DAOMFY010000504 DAOMFY010000000
VERSION     DAOMFY010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46117_length_1566_cov_5.340172"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLB01_20495"
     CDS             complement(<1..181)
                     /locus_tag="PLB01_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79730.1"
                     /translation="MAAVFVLLGGLRALGAEWAAAGGGETDPPLRHWEAPPFWRQLEA
                     APGREALAGRTALAGG"
     gene            complement(243..>1566)
                     /locus_tag="PLB01_20500"
     CDS             complement(243..>1566)
                     /locus_tag="PLB01_20500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ79731.1"
                     /translation="GLDRADEVGRLSRALRNVERRMRWRTASMEGVHRLGQTAALMTR
                     PDETLAQLARQIAQLVGATKGWLALWDPDTRSLVGAPPGWGIPEDALRGVRAGLDDPT
                     LAVIVYRTGETVLRNDLSDPRISQALVQRLEVRENFVLAPLRAESGILGVLGVADKPG
                     GFDAEDQAAIEGYADQAALLLRNARLYEELQKSYERLRDAQRNRDYFLQNVNHELRTP
                     LTAILGWSEVLAEDRPDKETVKIAVDQINRSAQFLLALISDLLDLSRYEEGRTRLEPE
                     IVDLGPLVAGAVEPVSVMAEAKGIAMSVETPAAPTPVRLDPLRMRQVLWNLVHNAVKF
                     TPKGGRIRVEASADDAGVTFSVVDNGVGVDAKDLPFIFERFRQADGSATRAYRGMGIG
                     LALAKAYVELHGGTISVESQPGKGTAFHIRIPRVPTSGPAGLPVPPVV"
BASE COUNT          210 a          559 c          579 g          218 t
ORIGIN      
        1 ggccgcctgc gagcgccgtc cggccggcga gcgcctcgcg gcccggcgcg gcctcaagct
       61 gccgccagaa cggaggcgct tcccagtgtc ggagcggggg gtccgtctcg ccgcccccgg
      121 ccgccgccca ctccgccccc agcgccctca gtcccccaag caggacgaac acggcagcca
      181 ttccgacctt ccgcatacga gcccccctgg gcatctgaag cgcagaaggg gcgagaatcc
      241 cctcagacga ccggcggaac cgggaggccc gcggggccgg acgtagggac ccgggggatc
      301 cggatgtgga acgccgtgcc ctttccgggc tggctctcca ccgagatcgt gccgccgtgg
      361 agctcgacgt aggccttcgc gagcgcgagg ccgatcccca tgccgcggta ggcgcgcgtg
      421 gccgagccgt ccgcctgccg gaaccgctcg aagatgaacg ggaggtcctt cgcgtcgacg
      481 ccgaccccgt tgtcgacgac cgagaacgtg acgccggcgt cgtcggcgga ggcctccacg
      541 cggatgcgcc cgcccttcgg cgtgaacttg acggcgttgt ggacgaggtt ccagagaacc
      601 tgccgcatcc gcagggggtc gagtcggacg ggcgtcggag cggccggcgt ctcgaccgac
      661 atcgcgatcc ctttcgcctc cgccatgacg gacaccggct ccaccgcccc cgcgacgaga
      721 gggccgaggt cgacgatctc cggctcgaga cgcgtgcggc cctcctcgta gcgcgagagg
      781 tcgaggagat ccgagatgag cgcgagcagg aactgcgccg agcggttgat ctggtcgacc
      841 gcgatcttga cggtctcctt gtcgggccgg tcctcggcga ggacctcgct ccagccgagg
      901 atcgcggtga gcggcgtgcg gagctcgtgg ttcacgttct ggaggaagta gtcgcggttc
      961 cgctgggcgt cgcgcaggcg ctcgtagctc ttctggagct cctcgtagag ccgcgcgttg
     1021 cgcaggagca gggccgcctg gtccgcgtag ccttcgatcg ccgcctggtc ctcggcgtcg
     1081 aaaccgcccg gcttgtccgc cacgccgagg acgccgagga ttccggactc ggcccggagc
     1141 ggcgcgagga cgaagttctc gcgcacctcg aggcgctgca ccagcgcctg cgagatgcgc
     1201 gggtcgctca ggtcgtttct gaggacggtc tctccggtcc ggtagacgat caccgcgagc
     1261 gtcgggtcgt cgaggccggc gcggacgccg cggagcgcgt cctcggggat gccccagccc
     1321 ggcggcgcgc cgacgagcga gcgcgtgtcc gggtcccaga gagcgagcca tcccttcgtg
     1381 gcgccgacga gctgcgcgat ctgccgcgcg agctgggcga gcgtttcgtc gggccgcgtc
     1441 atgagcgctg cggtctgccc gaggcggtgg acgccttcca tggaggcggt ccgccagcgc
     1501 atgcggcgct cgacgttccg cagggcgcgc gagaggcggc cgacctcgtc ggcgcggtcg
     1561 aggccc
//
LOCUS       DAOMFY010000505         1542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_47466_length_1542_cov_5.710827, whole genome shotgun sequence.
ACCESSION   DAOMFY010000505 DAOMFY010000000
VERSION     DAOMFY010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47466_length_1542_cov_5.710827"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PLB01_20505"
     CDS             complement(<1..1515)
                     /locus_tag="PLB01_20505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HQQ79732.1"
                     /translation="MHPEEFGRRFGAPEADVAALRSHLEAHGLEVEDVPAGRTALLFS
                     GRAADVERAFATELREVYVDGAVRVTNVRPARLPAALALRTAGLLSLNSFPRRKALAR
                     RTPAYTDGSGRHILAPADFATIYGTDTLAAAGINGAGRKIAIIAQTNVTVADTRFFRQ
                     YFGLPANDPQVVLNGPDPGINGDEIEADLDIQWAGAVAPAAQVLLVVSKTTLPSYGVD
                     LSALYAVNNNVADVLNLSYGACESDFVDSDTVFYTNIWAQAAAQGITSFVASGDSGAD
                     TCAQGGTSGSIRSVNGLGSSPYATCVGGTQFLDTASPSSYWSSVNDPVTKKSVRGPIP
                     EGAWNESSAGSVLAATGGGLSVLFTRPPWQNVAGVPAGSGRAVPDVSLAAAGHTPYYI
                     VMGGSGSASGLARVYGTSASAPAFAGIAALLSQRAGSRLGNLNPLLYALGQTQYGTGG
                     AASPGSGPFRDVTSGTNSVPGVTGYDAGPGYDAATGLGSPDVPALAAALPSPVTA"
BASE COUNT          225 a          538 c          576 g          203 t
ORIGIN      
        1 cgccgtgacg ggcgacggga gcgccgcggc gagagccggg acgtcgggcg agccgaggcc
       61 cgtcgcggcg tcgtagccgg ggcccgcgtc gtagcccgtg acgccgggga ccgagttcgt
      121 accgctcgtg acgtcgcgga acggaccgct gcccggcgac gcggccccgc ccgtcccgta
      181 ctgggtctgg cccagcgcgt acaggagggg gttcaggttg ccgaggcgcg agccggcgcg
      241 ctgcgagagc agcgccgcga tgccggcgaa ggccggggcc gacgcggagg tgccgtagac
      301 gcgcgcgaga cccgaggcgc ttcctgagcc gcccatcacg atgtagtacg gcgtgtggcc
      361 ggccgcggcg agcgaaacgt ccggcacggc gcggcccgat cccgccggaa ccccggcgac
      421 gttctgccag gggggccgcg tgaagagcac gctcagcccg ccgcccgtgg cggccaggac
      481 gctgccggcg ctgctctcgt tccacgcacc ctcggggatc gggccccgga ccgacttctt
      541 cgtgacggga tcgttcacgg aactccagta agaagacgga ctcgccgtgt cgaggaactg
      601 cgtacccccg acgcacgtcg cgtacggcga ggagccgagg ccgttcacgg aacggatgga
      661 cccgctcgtt cccccctggg cgcacgtatc ggccccgctg tcgccggacg ccacgaagga
      721 cgtgatcccc tgtgccgccg cctgcgccca gatgttcgtg tagaacaccg tgtccgaatc
      781 gacgaagtcg ctctcgcagg ctccgtagga cagattgagg acgtcggcga cgttgttgtt
      841 caccgcgtac agcgcggaga ggtcgacgcc gtagctcgga agcgtcgtct tcgacaccac
      901 gagcagcacc tgcgcggccg gcgcgacggc gcccgcccac tggatgtcga gatcggcctc
      961 gatctcgtcc ccgttgatcc cggggtcggg gccgttcagg acgacctgcg ggtcgttcgc
     1021 cggcaggccg aagtactgcc ggaagaacct cgtgtcggcc accgtcacgt tcgtttgggc
     1081 gatgatggcg atcttgcgtc ccgcgccgtt gatcccggcg gcggcgagcg tgtcggtccc
     1141 gtaaatcgtc gcgaaatccg cgggcgcgag gatgtggcgg ccggagccgt ccgtgtacgc
     1201 gggggtccgc cgcgcgagcg ctttgcgccg cgggaacgag ttcagggaga ggaggccagc
     1261 ggtccgcaag gcgagcgcgg cgggcagccg cgccggccgt acgttcgtga cccggaccgc
     1321 gccgtccacg tacacctcgc ggagctcggt cgcgaaggcc cgctccacgt cggcggcgcg
     1381 gccggaaaac aggagcgccg tgcggcccgc cgggacgtcc tccacctcca ggccgtgcgc
     1441 ctcgagatgg gaccgcagcg ccgcgacgtc cgcttcgggc gcgccgaaac ggcggccgaa
     1501 ctcctcggga tgcagacgaa gatcgcgtcg gttgaaaagg ga
//
LOCUS       DAOMFY010000506         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_47860_length_1535_cov_5.754054, whole genome shotgun sequence.
ACCESSION   DAOMFY010000506 DAOMFY010000000
VERSION     DAOMFY010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47860_length_1535_cov_5.754054"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLB01_20510"
     CDS             <1..206
                     /locus_tag="PLB01_20510"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQQ79733.1"
                     /translation="AIDAAHLELAAPSDPAALLAAAAHAGWTLRQLTDAYLRLVLEDS
                     GGNVAEAARRLGIARKTLYERRG"
     gene            213..683
                     /locus_tag="PLB01_20515"
     CDS             213..683
                     /locus_tag="PLB01_20515"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQQ79734.1"
                     /translation="MRIRFLFVGKSEDAEYARGVERYVARIARFAPCDVVVVKEEKPG
                     AKADAGRVKTKEGERLLGALQPRDLLVLCDERGKERTSAEFARDLRGWLDSGPSAVVF
                     AVGGAFGLDPSLAPRARAILSLSRMTLPHQMARLLLAEQVYRATATLSGVAYSK"
     gene            701..1090
                     /locus_tag="PLB01_20520"
     CDS             701..1090
                     /locus_tag="PLB01_20520"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HQQ79735.1"
                     /translation="MSPKSALAPAKFSKKESGEKVKKRLHAKRDELLADLAKNREVSD
                     ETVDESAQDMADRATSAYTKEFAYSLSESDRKILLLIDESLGRLDAGTYGTCVNCGQP
                     VQEKRLEAVPWARHCLDCQELQDKGLL"
     gene            1109..>1535
                     /locus_tag="PLB01_20525"
     CDS             1109..>1535
                     /locus_tag="PLB01_20525"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79736.1"
                     /translation="MKRPEPFAEIGPLLSAARTAPLPPLFFVTGDDDWIVAEAVRRIA
                     AAFREAFREGEVTSYEAPGGGVAEAVADAATVALFSTNRLVTLEATDLLRGKGLTADE
                     LDALLDEAAEAGEDARAFTRLARRARAIAAAAGIPASDVA"
BASE COUNT          231 a          553 c          538 g          213 t
ORIGIN      
        1 ccgcgatcga cgccgcgcac ctcgagctcg cggccccgtc cgacccggcg gcgctactgg
       61 cggccgccgc gcacgcgggc tggactctcc ggcagctgac ggacgcgtac ctccgcctcg
      121 tcctcgagga ctccggagga aacgtcgcag aggcggcgcg ccgcctggga atcgcgcgga
      181 agaccctcta cgaacgccgg ggctgagacc ggatgcgcat ccgcttcctc ttcgtcggaa
      241 agagcgagga cgccgagtac gcgcgcggcg tcgagcgcta cgtggcgagg atcgcgcgct
      301 tcgcgccctg cgacgtcgtc gtcgtcaagg aggagaagcc cggcgcgaag gcggacgccg
      361 ggcgcgtgaa gacgaaggag ggcgagcgcc ttctcggggc cctccagccc cgcgaccttc
      421 tggttctctg cgacgaacgg ggaaaggagc ggacgagcgc cgagttcgcc cgggacctgc
      481 gcgggtggct cgattccggg ccgtccgcgg tggtgttcgc ggtcgggggg gcgttcggtc
      541 tcgacccctc tctggctccg cgggcgcgtg ctatcctctc gctttcccgg atgaccctgc
      601 cccaccagat ggcacggttg ctcctggcgg agcaggtcta ccgggcgacc gcgacgctct
      661 ccggcgtcgc gtattccaag tgacctcgag gaaaaccacg atgtctccca agtcggcgct
      721 tgcacccgcg aaattctcca agaaggaatc cggcgagaag gtcaagaaac gccttcacgc
      781 caaacgcgac gagctcctgg cggacctcgc caagaaccgc gaggtctccg acgagaccgt
      841 cgacgagtcc gcccaggaca tggccgaccg cgccacgtcg gcctacacga aggaattcgc
      901 gtactcgctc tccgagagcg accgcaagat tctgctcctg atcgacgagt ccctcggccg
      961 gctcgacgcg ggcacctacg gcacgtgcgt caactgcggc cagcccgtcc aggaaaagcg
     1021 gctcgaggcc gtgccctggg cgcgccactg cctggactgc caggagcttc aggacaaggg
     1081 ccttctctaa gcggttagga tgggcgccgt gaagcgcccg gagcccttcg ccgagatcgg
     1141 gccgctgctc tcggcggcgc ggacggcacc ccttccgccc ctcttcttcg tcacgggaga
     1201 cgacgactgg atcgtcgcgg aggccgtgcg ccggatcgca gccgcgttcc gcgaggcctt
     1261 ccgcgagggc gaggtcacgt cctacgaggc ccccggcgga ggggtcgccg aggccgtcgc
     1321 cgacgccgcg accgtcgcgc tcttctcgac gaaccgcctc gtgacgctcg aggcgacgga
     1381 tctccttcgg ggcaagggcc tgacggcgga cgagctcgac gcgctcctcg acgaggccgc
     1441 cgaagcgggg gaggacgcgc gtgcgttcac gcgtctcgcg cgccgggcgc gcgcgatcgc
     1501 ggcggccgcc ggtatccccg cgtccgacgt cgccg
//
LOCUS       DAOMFY010000507         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_47918_length_1534_cov_6.918864, whole genome shotgun sequence.
ACCESSION   DAOMFY010000507 DAOMFY010000000
VERSION     DAOMFY010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47918_length_1534_cov_6.918864"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..521
                     /locus_tag="PLB01_20530"
     CDS             129..521
                     /locus_tag="PLB01_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ79737.1"
                     /translation="MTGLPNALAGRRVLVVDDEPYIQKILSFKLRLTGLDPVEAASGE
                     EALRLVREALPHLVLLDVSLTPGLTGFDVCRILKENPATAGIPVIMLTARTLPAERDL
                     GLRLGAASYVTKPFSTKVLVQEIEKALS"
     gene            complement(890..1300)
                     /gene="rplQ"
                     /locus_tag="PLB01_20535"
                     /pseudo
     CDS             complement(890..1300)
                     /gene="rplQ"
                     /locus_tag="PLB01_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1308..>1534)
                     /locus_tag="PLB01_20540"
     CDS             complement(1308..>1534)
                     /locus_tag="PLB01_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HQQ79738.1"
                     /translation="LDTNVEELELSVRSANCLKNAGIRTLRELVQKTEKDMLETKNFG
                     RKSLNEIKDILREKGLAFGMKLEAPNGVGR"
BASE COUNT          195 a          539 c          506 g          294 t
ORIGIN      
        1 tcgggcgcgc ggtggcccac ggcgcgcggc tcggggtcac gggcgcccac gtcacgatca
       61 cgcacggcag ggagatcgcg gcggccgtcg ttctcctcac gggcaggccc ctctagaatg
      121 gcgcccgcat gacgggactt ccgaacgcgc tcgcgggcag gagggtgctc gtcgtcgacg
      181 acgagcccta catccagaag atcctgtcgt tcaagctgcg cctcacgggg ctcgacccgg
      241 tcgaggcggc gagcggcgag gaggcgttgc ggctcgtccg tgaggcgctg ccccacctgg
      301 tcctgctcga cgtttcgctg acgccggggc tcacgggctt cgacgtctgc cgcatcctca
      361 aggagaatcc ggcgacggcc ggaatcccgg tcatcatgct cacggcccgc acgctgcccg
      421 ccgagcgcga cctcggcctg cgcctcggcg ccgcgagcta cgtgacgaag ccgttctcga
      481 cgaaggtcct cgtgcaggag atcgagaaag cgctcagcta ggaaggccga agagccgcgc
      541 tgcgcgcggc cgggggatcc tcttagaagg tcaatagggc gcgccgcacc gcccgcccca
      601 tgaatgaaaa ggccccggtc tcccggggcc ctcttaacct tctaggaaat cttccgttct
      661 cttcggacct tggagactag ctgccgacct tccggtggcc gccggcgtgg ccgccgccgc
      721 ccttcgggcc cgagcccttg gggcccttga tcttcgcggc tttcggcttc tcggcgggct
      781 tcgccttctt gtcggcctcg ccctcgccgc cttcggcccc ttcggccttc ttctcgtcgc
      841 cgcccttgcc ggacacgagc ttcttggcct tctcgaggag cgaggccttc ttctcgtccg
      901 cggccttcgc cgcgaccttc ttctcggcga cgcgctgtgc gaaatcgaag tcgatgaact
      961 cgaggatcgc catctcggcg ccgtcgccct ggcggcggcc gagcttgagg atccgcgtgt
     1021 agccgccggc gcgctccttg aagcgcgggg cgatctcctc gaagagcttc ttgaccgtcg
     1081 cggcctcggg gatcgtcttc agcgcgagcc ggcgcgcgtg cagcccgccc cgcttgccga
     1141 gggtgaccat cttctcgatg agcggccgga ggtctttcgc cttcggcagc gtcgtctgga
     1201 tgcgttcgtg cgtgatgagg gacgagagct ggttccggaa caacgccttg cggtgcgccg
     1261 tcgtccggcc gagcttgcgg cccgcgcgat tgtgctgcat cgaatcctca gcgtcccacg
     1321 ccgttcgggg cttcgagctt catgccgaag gcgagcccct tctcgcgaag gatgtccttg
     1381 atctcgttca ggctcttgcg cccgaagttc ttcgtctcga gcatgtcttt ttccgtcttc
     1441 tggacgagct cgcggagcgt gcggatgccg gcgttcttca ggcagttcgc gctgcgcacg
     1501 gacagctcga gctcctcgac gttcgtgtcg agca
//
LOCUS       DAOMFY010000508         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_48361_length_1527_cov_7.905571, whole genome shotgun sequence.
ACCESSION   DAOMFY010000508 DAOMFY010000000
VERSION     DAOMFY010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48361_length_1527_cov_7.905571"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>1527
                     /locus_tag="PLB01_20545"
     CDS             153..>1527
                     /locus_tag="PLB01_20545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ79739.1"
                     /translation="MPDGGGPPVPERIDHYTIVRRLALGGMAEIFLAVDAKTGRKVAI
                     KKILPHLLTDPDFLDRFFHEIRIQIGLKHPNIVELVDCSPTPSNAYIVMEYVDGGELY
                     ALRQESGRFPWEVALYAVDEALNGLAAAHAKGVVHRDIKPQNIMWTKDGSVKIGDFGI
                     SHAEHLTRLTMTGTVVGTPAHMSPEQARGDELDARTDLFSMGTVLYELLCGFNPFTAD
                     SIAVTLRRVAETEPELPSLLDPTIPPGVDTFLRKLHAKDRSARFPDAATASRALRAVF
                     EKENVERPAVLFRSFLDDPGAFVAARNRRLAAESAAAAETLLKDSSARPEEALWAAYR
                     TVACLPHDPKAQELFRTAAIRAGQREKPLDNAKIRSLEEALKKEPDNLALLLQLAKLY
                     RLEHDFVNLMRFFRKLQALAPPDPYTQGQIASLLSSDAGSARALPASVPASPRTVPAA
                     PPARTGPR"
BASE COUNT          230 a          595 c          484 g          218 t
ORIGIN      
        1 cgctcggcgc tcctcgcgtt tctcctcgag gcccgcctcg gagtcgtcgc cgcgaactac
       61 gacggttcgc tctgggaatg gtccgcgcgc gaggacctgc cgatggaggc cggcccgcct
      121 ccctgagcgg gcccgcgtaa gattcccgca cgatgcccga cggcggcggc cctccggttc
      181 cggagaggat cgaccactac acgatcgtcc gccggctcgc actcggcggg atggcggaga
      241 tcttcctcgc cgtcgacgcg aagaccggcc gcaaggtcgc gatcaagaag atcctcccgc
      301 acctgctcac ggaccccgac ttcctcgacc gtttcttcca cgagatccgg atccagatcg
      361 ggctcaagca cccgaacatc gtcgagctcg tcgactgcag cccgacgccg tcgaacgcgt
      421 acatcgtcat ggagtacgtc gacggaggcg agctctacgc cctgcgccag gagtcggggc
      481 gcttcccgtg ggaggtcgcg ctctacgccg tcgacgaggc cctgaacggc ctcgcggccg
      541 cgcacgcgaa gggcgtcgtc caccgcgaca tcaagccgca gaacatcatg tggacgaaag
      601 acggctccgt gaagatcggc gatttcggca tcagccacgc cgaacacctc acgcgcctca
      661 cgatgaccgg gaccgtcgtc gggacgccgg cgcacatgtc gcccgagcag gcgcgcggcg
      721 acgagctgga cgcacgcacc gacctctttt cgatgggcac cgtgctctac gagctcctct
      781 gcgggttcaa cccgttcacg gccgattcga tcgccgtgac gctccgccgc gtcgcggaga
      841 ccgagcccga gcttccctcg ctgctcgacc cgacgatccc gcccggcgtc gacacgttcc
      901 tccggaagct ccacgccaag gaccgctcgg cccgcttccc cgacgcggcg acggcctcgc
      961 gggccctgcg ggcggtcttc gagaaggaaa acgtcgagcg ccccgccgtt ctcttccgct
     1021 cctttctcga cgacccgggc gccttcgtcg ccgcgcgcaa ccggcgcctc gccgcggagt
     1081 ccgcggcagc ggccgagacg ctcctgaagg attcctcggc gcggcccgag gaggccctct
     1141 gggccgccta ccgaacggtg gcgtgcctcc cgcacgaccc gaaggcgcag gagctcttcc
     1201 ggaccgccgc gatccgcgcc gggcagcgcg agaagccgct cgacaacgcg aagatccgct
     1261 ccctcgagga ggcgctcaag aaggagcccg acaatctcgc gctccttctg cagctcgcga
     1321 agctctaccg cctcgagcac gacttcgtca acctcatgcg gttcttccgc aagctccagg
     1381 cgctcgcgcc gcccgacccg tacacgcagg ggcagatcgc ctcgctgctc tcgtcggacg
     1441 cggggtccgc gcgagcgctg ccggcgtcgg ttcccgccag cccgcgcacg gttcccgccg
     1501 cgccgcccgc gcggacgggt ccgcgcg
//
LOCUS       DAOMFY010000509         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_48363_length_1527_cov_5.360054, whole genome shotgun sequence.
ACCESSION   DAOMFY010000509 DAOMFY010000000
VERSION     DAOMFY010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48363_length_1527_cov_5.360054"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /gene="tilS"
                     /locus_tag="PLB01_20550"
     CDS             complement(<1..863)
                     /gene="tilS"
                     /locus_tag="PLB01_20550"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQQ79740.1"
                     /translation="MAETLPDRFARHLAETGLLDGAGVLAVACSGGGDSTALLLLVHG
                     WAVPRGVAVVALHVAHGLRGDAGLRDARFCAELAARLGVPYGFLSVDVPEQRKKGESV
                     ESAARRLRYRALLSTAGEMEALVLTGHTRDDQAETVLLHLERRLGRGRGGIRAKRPDG
                     VVRPLLPFSRSELRAYLEGRGVPWREDETNENEGFARNRVRRTVLPGIERRMPGATER
                     LARAGEAYSARLDALDRRLDRMLGEEKIPSKGNWPRSFFDRLTDEETARFLVRAAGAG
                     GPTRAGPPPRRA"
     gene            910..986
                     /locus_tag="PLB01_20555"
     tRNA            910..986
                     /locus_tag="PLB01_20555"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:944..946,aa:Met,seq:cat)
     gene            1019..1273
                     /locus_tag="PLB01_20560"
     CDS             1019..1273
                     /locus_tag="PLB01_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79741.1"
                     /translation="MRRKETEEDANSPYLFCEQCEQMYERLLIQECPYCEKRFCRACA
                     VRSGQQQFCGKACAKNWFFSDEEEESEDAVERGELDKGDV"
     gene            1292..>1527
                     /locus_tag="PLB01_20565"
     CDS             1292..>1527
                     /locus_tag="PLB01_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HQQ79742.1"
                     /translation="MPVADAALREALARPRAALSGSRDLRWVQGEQLHLTLKFFADLP
                     PALADSVAAAAAAASAAAAPFDLEVRGLGRFPAHG"
BASE COUNT          202 a          553 c          555 g          217 t
ORIGIN      
        1 gcccgtctcg gcggcggtcc ggcgcgcgtc ggccccccgg cccccgccgc tcggaccaga
       61 aagcgcgccg tttcctcgtc cgtgagccgg tcgaagaagc tccggggcca gttacctttc
      121 gaaggaatct tctcttctcc gagcattctg tccagccgcc tgtcgagcgc gtcgagccgg
      181 gcggagtacg cctcgcccgc ccttgcgagc cgttccgtcg cgcccggcat gcgccgctcg
      241 atgcccggca ggacggtgcg ccggacgcgg ttgcgcgcga agccttcgtt ctcgttcgtc
      301 tcgtcctcgc gccacgggac gccccggcct tcgaggtacg cgcgcagctc ggagcgcgag
      361 aaggggagga ggggccgcac gacgccgtcg gggcgcttcg cgcggatgcc gccgcgcccg
      421 cggccgagcc tgcgttcgag gtggaggagg acggtctcgg cctggtcgtc gcgcgtgtgt
      481 ccggtgagga cgagggcctc catttcgccg gccgtggaca ggagcgcgcg gtaacgcagc
      541 cgccgcgcgg ccgactcgac gctttcgccc ttcttcctct gctccgggac gtcgacggac
      601 aggaaaccgt acgggacacc gaggcgcgcg gccagctccg cgcaaaaccg cgcgtcgcgg
      661 agacccgcgt cgcctcgcag gccgtgggcg acatggagcg ccacgaccgc gacgcctcgc
      721 ggcaccgccc agccgtggac gaggaggaga agcgccgtcg agtcgccgcc gcccgaacag
      781 gcgacggcga gaacgccggc cccgtcgagg aggccggttt ccgcgagatg ccgggcgaag
      841 cggtccggga gggtttcggc catgctgcgc tatcatccgc ccctcgcccg ggccccggga
      901 gcgatcaggg gcggcgtagc tcagatggcc agagcagggg tctcataagc cctttgtcgg
      961 cggttcgatt ccgcccgccg ccaccacttc cgtccttccg ccgccgtatg attcccgcgt
     1021 gcggcggaaa gagaccgagg aagacgcgaa ctccccgtac ctgttctgcg agcaatgcga
     1081 gcagatgtac gagcgcctcc tgatccagga gtgcccgtac tgcgagaaga ggttctgccg
     1141 cgcgtgcgcg gtccgctccg gccagcagca gttctgcggc aaggcctgcg cgaagaactg
     1201 gttcttctcg gacgaggagg aggagtcgga ggacgccgtg gaacgcgggg aactcgacaa
     1261 gggagacgtc tagatccgcg cgttcgtcgc cgtgcccgtc gcggacgcgg ccctccggga
     1321 ggccctcgcg aggccgcgcg cggccctctc ggggtcgcgc gacctgcgct gggtgcaggg
     1381 cgagcagctc cacctgacgc tgaagttctt cgccgatctc ccgcccgcgc tcgcggactc
     1441 cgtcgccgcg gccgcggccg cggcgtctgc ggcggccgcc ccgttcgacc tcgaggtgcg
     1501 cggcctgggg cgattcccgg cgcacgg
//
LOCUS       DAOMFY010000510         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_48788_length_1520_cov_4.787713, whole genome shotgun sequence.
ACCESSION   DAOMFY010000510 DAOMFY010000000
VERSION     DAOMFY010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48788_length_1520_cov_4.787713"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PLB01_20570"
     CDS             <1..1218
                     /locus_tag="PLB01_20570"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ79743.1"
                     /translation="RPEGRAPGKLLIVVPSRAEGARVGDLPGDVRREISLKENTAAAA
                     SGAAPVMPRVHVDVLVVLDGPDADAEARLKADGVDYLSKKSAGPAKGDALAFLAEQMD
                     DRLDAYDFILVFDADMRLPEGFFRDLRVPGGTEAFQLPVRPSGVPAPGAPRVEALSLA
                     EARLDDLARDAAGLPVRLRGKAMGFSPRAFRLGPAAATRTTAEDSEATLALLAKGIVV
                     RALDGPMAFDEPSSDAGAMGRSRARWFGGHMKLLFSGLGDLVRSFVRRPVGTLVLAAD
                     LWLRPRIFLLGTLAFLAGASDLAMVVFSSSGAWSASAHAAGGAAAAGGATGAAMPVAL
                     LVCLLLLSLEGKAALLFEWLSLAAVRHRIGFPAECPPVALADLSDSLLVWAGAVVRGV
                     LAPSRWHRARPEA"
     gene            1215..>1520
                     /locus_tag="PLB01_20575"
     CDS             1215..>1520
                     /locus_tag="PLB01_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79744.1"
                     /translation="MKAVLLVPRLPGTGHTGDRLRAELHLAALASAGFETAVVGGTET
                     GDFLRMKNAAAVHPVRLRRALIPAGLASAVLSGEPLQSALFAGPFGVALREAARDADL
                     "
BASE COUNT          160 a          529 c          548 g          283 t
ORIGIN      
        1 cggccggagg gccgcgcgcc cgggaagctc ctcatcgtcg ttccctcgcg ggccgagggc
       61 gcgagagtcg gcgatcttcc gggcgatgtt cgaagagaga tttctttgaa ggagaatacg
      121 gcagccgctg ccagcggcgc cgcgcctgtg atgcctcggg tgcacgtcga cgttctcgtc
      181 gttctggacg gccccgacgc cgatgccgag gcccgtctca aagcggacgg cgtggattac
      241 ctttctaaga aatctgccgg tccggcgaag ggagacgcac tggcgtttct cgccgagcaa
      301 atggacgacc ggctggatgc ctacgatttc atcctcgtct tcgacgccga catgcgtttg
      361 cccgagggct tcttccgcga cctccgcgtg cccgggggga cggaggcgtt ccagctgccg
      421 gtccgtcctt cgggcgtccc ggcgccgggc gcgccgcgcg tcgaggcgct gtcgctggcc
      481 gaggctcgcc tcgacgacct cgcacgcgac gcggcgggcc ttcccgtgcg cctgcgcggc
      541 aaggcgatgg gcttttcgcc gcgggcgttc cgcctcggcc cggcggccgc gacgcgcacg
      601 accgccgagg actccgaggc gacgctcgcg ctcctcgcca agggaatcgt cgtgcgcgcg
      661 ctggacgggc caatggcctt cgacgagccg tcgtccgacg cgggcgcgat ggggcgctcc
      721 cgggcgcgct ggttcggcgg gcacatgaag ctgcttttct cgggtcttgg agatctcgtt
      781 cggagcttcg tgaggcggcc ggtcgggact ctcgtgctgg cggcggatct ctggctccgt
      841 ccgaggatct ttctgcttgg gaccctcgcg tttcttgcag gcgcctcgga tctggcgatg
      901 gtggtttttt cttcgagtgg cgcgtggtct gcctcggcgc atgcagccgg cggcgccgcg
      961 gcggccgggg gcgcgactgg ggcggccatg cccgtcgcgc tcctggtctg cctcctgctc
     1021 ctttcactcg aagggaaggc ggccttgctc ttcgagtggc tttctctcgc cgcggttcgc
     1081 catcgcatcg gattcccggc ggaatgtccc cctgttgctc tcgccgatct ctccgactct
     1141 cttctcgtgt gggcgggcgc cgtcgtccgc ggcgtgctcg caccgtcgcg gtggcatcgc
     1201 gcgaggcccg aggcatgaag gccgtcctcc tcgtcccgcg cctgccgggc acgggccaca
     1261 cgggcgaccg cctgcgcgcc gagctgcacc tcgcggcgct cgcgtcggcg ggcttcgaga
     1321 cggccgtcgt cgggggaacg gagacggggg atttccttcg aatgaagaac gcggcggccg
     1381 ttcatcccgt gcggctccgt cgggccctga ttcctgcggg gctcgcgtcg gcggtgcttt
     1441 ccggcgagcc gctgcagtcc gcgctcttcg caggaccttt cggcgtcgcc ctgcgcgaag
     1501 cggcccgtga cgcggacctc
//
LOCUS       DAOMFY010000511         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_49258_length_1511_cov_6.909341, whole genome shotgun sequence.
ACCESSION   DAOMFY010000511 DAOMFY010000000
VERSION     DAOMFY010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49258_length_1511_cov_6.909341"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PLB01_20580"
     CDS             complement(<1..1284)
                     /locus_tag="PLB01_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79745.1"
                     /translation="MAGTKRDALIASAEKSLQKGKVDSALKDYLKVLEETPGDINILN
                     KVGDLYTRLNRNEEAIPYLTRIAEHFARDGFFLRGIAIYKRINRLDPARLDVYERLAE
                     LYAKQGLTTDAKSQYQVLADYHARNDNAIGAIGIYQKMVGIDPQNIQLHVKLADFFTQ
                     ARRKPDALKEYTVVAGLLKDRGAVDEAIQVYEKALQMAPDNVEVLRSFVPLLVSSSRA
                     REARLFVKRALDTTPRSMPLFLLAAEAALQDGDVKDAQEWLGKAQAIEASSEEVLQLA
                     VRVQQAGGTATLTFEAAAALADQAMRRGEAKKALGILVPLAESSLDREDVLARVVHVA
                     EAAGDQAAVIHWRSALVDLHRKQNRIGDAAEGLRALLKLVPDSAEFRTRLAQLDPSLG
                     RGLERSASRERPVIPPPPMETSGRRAQPAPAAPVRP"
     gene            complement(1288..>1511)
                     /locus_tag="PLB01_20585"
     CDS             complement(1288..>1511)
                     /locus_tag="PLB01_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQQ79746.1"
                     /translation="AAIAAASIVAKTARDARMDELDGVFPYYGFSSHRGYGTPEHLAA
                     LRRHGPSPEHRLTFDRVLARRRARASRAA"
BASE COUNT          201 a          505 c          560 g          245 t
ORIGIN      
        1 gggccggacg ggcgcagccg gcgcgggctg ggcgcgccgg ccgctcgtct ccatcggcgg
       61 cggcgggatc acagggcgtt cgcgggacgc gctgcgttcc aggccgcgcc cgagcgacgg
      121 atccagctgc gcgaggcgcg tgcggaactc cgccgaatcg ggaacgagct tcaggagagc
      181 acgaagacct tccgcggcgt cgccgatccg gttctgcttc cggtggaggt ccacgagcgc
      241 cgaacgccag tggatcaccg cggcctggtc ccccgcggct tcggccacgt ggaccacgcg
      301 ggcgagaacg tcctcgcggt cgagggagga ctcggcgagc ggcacgagga tcccgagtgc
      361 cttcttcgcc tcgccgcggc gcatggcctg gtcggcgaga gcggcggcgg cctcgaacgt
      421 cagcgtggcg gtgcctcccg cctgctggac gcgcaccgcg agctggagga cttcctcgct
      481 cgacgcctcg atggcctgcg ccttcccgag ccactcctgc gcgtccttca cgtcgccgtc
      541 ctggagggcc gcctcggccg cgagcaggaa caggggcatg gagcgcggcg tcgtgtcgag
      601 agcgcgcttg acgaagagcc gcgcctcgcg ggcccgcgac gacgagacga ggagcgggac
      661 gaacgaccgc aggacctcga cgttgtcggg cgccatctgc agcgccttct cgtagacctg
      721 gatcgcctcg tcgacggctc cgcggtcctt gagaagcccg gcgacgaccg tgtactcctt
      781 cagcgcgtcg ggcttgcggc gcgcctgcgt gaagaagtcc gcgagcttga cgtgcagctg
      841 gatgttctgc gggtcgatcc cgaccatctt ctggtagatg ccgatggcgc cgatcgcgtt
      901 gtcgttccgc gcgtggtagt cggcgaggac ctggtactgg ctcttggcgt ccgtcgtgag
      961 cccctgcttg gcgtacagct ccgcgagccg ctcgtagacg tcgagtcgcg ccggatcgag
     1021 ccggttgatg cgcttgtaga tcgcaatgcc gcgcaggaag aagccgtcgc gcgcgaagtg
     1081 ctcggcgatc cgcgtgaggt acgggatcgc ctcctcgttg cggttgaggc gcgtgtagag
     1141 gtcgccgacc ttgttgagga tgttgatgtc gcccggcgtc tcctcgagga ccttcaggta
     1201 gtccttgagg gccgaatcga ccttgccttt ctgaaggctc ttctcggccg aggcgatgag
     1261 agcgtcgcgc ttcgttccgg ccatggttca ggccgcccgc gacgccctgg cgcggcggcg
     1321 cgcgaggacg cggtcgaagg tgagtcggtg ctcggggctc ggcccgtggc gcctcagcgc
     1381 cgcgaggtgc tccggcgtcc cgtagccgcg gtgcgaggaa aaaccgtagt acggaaagac
     1441 gccgtcgagc tcgtccatgc gcgcgtcgcg cgccgtcttg gcgacgatcg acgccgccgc
     1501 gatggccgcg a
//
LOCUS       DAOMFY010000512         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_4990
            NODE_49514_length_1507_cov_6.435262, whole genome shotgun sequence.
ACCESSION   DAOMFY010000512 DAOMFY010000000
VERSION     DAOMFY010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867149
            Sequence Read Archive: SRR5278466
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:16:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,119
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 4,039
            CDSs (with protein)               :: 4,039
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 1 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49514_length_1507_cov_6.435262"
                     /isolate="AMR_MDS_4990"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2016-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLB01_20590"
     CDS             complement(<1..870)
                     /locus_tag="PLB01_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ79747.1"
                     /translation="MIRPAAALKSRPRFACAAALGLAAAGEAVVRTGHAWYAGAALTL
                     AGALLLAPGAWPAPEPGGAPVPASRGGRILAAFAALAAAFGVAAAVLLWRESEGPSFT
                     LWRLSLAALFAASVAAGRGLDVPERWDAARLPAGRSSRLALLGFGAVLLAAAAGVRLW
                     NLAGVPFGVNPDEGDQAALAMQVAGGFNTDGWFAYGWYHLSMVYFKLLAAVMSVAGMD
                     VAGARVFGALCGLATVAVVTVLAIRHFGWRAGLLAGTLLSFMGGAIQFSRVTTVAAPT
                     QTLWALSAAGFLEA"
     gene            936..>1507
                     /locus_tag="PLB01_20595"
     CDS             936..>1507
                     /locus_tag="PLB01_20595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQQ79748.1"
                     /translation="MSTRGPALSFRRGLAVVFLLAAAVRIVGLDFDQNHFFHPDERAI
                     GEAIVHLSFVPPQLNPHFFAYGSFPFYVTRAAASALAAVTRREWFASYDGIIHVGRFL
                     SALWGAATCVLVALLGRRWYGEAAGLLAGGLLACSVLHVQTSHFAATDVALTFMVLLA
                     LVCDSRLANRGRVRDALVAGAATGLALATKA"
BASE COUNT          222 a          583 c          519 g          183 t
ORIGIN      
        1 cgcctcgagg aaccccgccg cgctgagcgc ccagagcgtc tgcgtgggcg cggcgacggt
       61 cgtgacgcgc gagaactgga tcgcgccgcc catgaacgag agcagcgtcc ccgcgaggag
      121 cccggcgcgc cacccgaagt gccggatcgc gaggaccgtc acgacggcga ccgtcgcgag
      181 gccgcagagg gcgccgaaca cgcgcgcacc cgcgacgtcc atcccggcca ccgacatgac
      241 ggccgcgagc agcttgaagt acaccatgct gaggtggtac cagccgtacg cgaaccaccc
      301 gtccgtgttg aagcctcccg cgacctgcat cgcgagcgcg gcctggtcgc cctcgtcggg
      361 gttcacgccg aagggaacgc ccgcgaggtt ccaaagcctc acacccgccg ccgcggcgag
      421 gagcacggcg ccgaacccga ggagcgcgag ccgcgagctc cggccggccg gaagacgtgc
      481 cgcgtcccag cgctccggga cgtcgagacc gcgccccgca gcgacggacg ccgcaaagag
      541 ggccgcgagc gagagacgcc agagcgtgaa tgacggccct tcggactcgc gccagagaag
      601 gacggcggcc gcgacaccga aagcggccgc gagagccgca aacgccgcaa ggattctccc
      661 gccccgggag gccggaacgg gcgcgcctcc aggttcgggc gcgggccacg cgcccggtgc
      721 gaggaggagg gcgccggcga gcgtcagcgc cgcgcccgcg taccaggcgt ggcccgtccg
      781 cacgaccgcc tcgcccgcgg ccgccagccc gagggctgcg gcgcacgcga agcgcggccg
      841 ggacttcagc gccgccgccg gtcggatcac ggggaatcaa ggtagcacag gctttcgccg
      901 gcgcggcgcc tcgccgataa tcggccccag tctccatgtc gacacgagga cccgccctga
      961 gcttccgacg cgggctcgcc gtcgtcttcc tcctcgccgc cgccgtgagg atcgtggggc
     1021 tcgacttcga ccagaaccac ttcttccatc ccgacgagcg cgcgatcggc gaggcgatcg
     1081 ttcatctctc gttcgtcccg ccccagctca acccgcactt cttcgcgtac ggctcgttcc
     1141 ccttctacgt gacgcgcgcc gcggcctcgg cgctcgcggc ggtgacgcgg cgcgaatggt
     1201 tcgcgtccta cgacggcatc atccacgtcg ggcgattcct gtccgcgctc tggggcgcgg
     1261 cgacatgcgt cctcgtcgcg ctgctcggca ggcgctggta cggcgaggcc gccggcctcc
     1321 tcgcgggagg gctcctcgcg tgttcggtcc tgcacgtcca gacctcccac ttcgccgcga
     1381 ccgacgtcgc cctcacgttc atggtcctcc tcgcactcgt ctgcgattcg cggctcgcga
     1441 accgcggacg ggtacgcgac gccctcgtcg cgggcgccgc aacgggtctc gcgctcgcga
     1501 cgaaagc
//
